LOCUS       DAZKIP010000001       114992 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZKIP010000001 DAZKIP010000000
VERSION     DAZKIP010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 114992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 114992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..114992
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.001"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..101)
                     /locus_tag="ACHOUK_000001"
     CDS             complement(<1..101)
                     /locus_tag="ACHOUK_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9895539.1"
                     /translation="MSRHDILLRSQFERIIEGDRVGQALISFYEKLP"
     gene            complement(247..4149)
                     /gene="hrpA"
                     /locus_tag="ACHOUK_000002"
     CDS             complement(247..4149)
                     /gene="hrpA"
                     /locus_tag="ACHOUK_000002"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFP9895540.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTGRDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRIGHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWFNKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            4350..4955
                     /gene="azoR"
                     /locus_tag="ACHOUK_000003"
     CDS             4350..4955
                     /gene="azoR"
                     /locus_tag="ACHOUK_000003"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFP9895541.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTTRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(5006..6298)
                     /gene="ynbD"
                     /locus_tag="ACHOUK_000004"
     CDS             complement(5006..6298)
                     /gene="ynbD"
                     /locus_tag="ACHOUK_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFP9895542.1"
                     /translation="MLQGAGWLLLLAPFFFFTYGSLNQFTAVQDFNSHDIPSQVFGWE
                     TAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCGFFLYPLKF
                     SFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIVLCWLLWRHFRQHLAVRWRKVCGGW
                     FLLIAISMLTTWQHHFIDVITGLAVGMLIDWMIPVDRRWNYQKPDQRRIKIALPYVVG
                     ACACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQGKLPPAVYW
                     LTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTFEFPRGRAT
                     KDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSALVVAAWLL
                     CYGHCKTVDEAISFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(6312..8069)
                     /gene="ynbC"
                     /locus_tag="ACHOUK_000005"
     CDS             complement(6312..8069)
                     /gene="ynbC"
                     /locus_tag="ACHOUK_000005"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFP9895543.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGQTSGPRGYSPSLARSVRDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYHRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGT
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPGNEQVKNSLAGLAN
                     AIEPGGILLYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(8085..8981)
                     /gene="ynbB"
                     /locus_tag="ACHOUK_000006"
     CDS             complement(8085..8981)
                     /gene="ynbB"
                     /locus_tag="ACHOUK_000006"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFP9895544.1"
                     /translation="MLEKSLATLFALLILATLINRFRVWRLPERKGDEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLLIGFSC
                     FELFLLFIPLAGFLILTTWRVLVGDTSGFLHTVSAIFWGWIMTVFALSHAAWLLMLTT
                     TNTQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAVKRDVGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            complement(8981..9586)
                     /gene="ynbA"
                     /locus_tag="ACHOUK_000007"
     CDS             complement(8981..9586)
                     /gene="ynbA"
                     /locus_tag="ACHOUK_000007"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFP9895545.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLITGL
                     LLMLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGIRSYVGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMSAER"
     gene            complement(9757..12063)
                     /gene="ydbD"
                     /locus_tag="ACHOUK_000008"
     CDS             complement(9757..12063)
                     /gene="ydbD"
                     /locus_tag="ACHOUK_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HFP9895546.1"
                     /translation="MFKPRLCSWIGLLPLFMLSLPVQAELRCVANAVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSTIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRLKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDGQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSHLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNDVVTSLALKLAEMKHHGQLLRM
                     TPQESDKIAVYLYQKFENDDDLIGALFLALPDNLQFNFVKRMEKKSPAYFCCRDMQII
                     HSDAALQRLLTRFNDPEGGSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSDAYETS
                     ADMILSELISYGEVDDQMLLNATSLIRSDDWDFLESALISWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVNEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALKLVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            complement(12127..12987)
                     /gene="pdxI"
                     /locus_tag="ACHOUK_000009"
     CDS             complement(12127..12987)
                     /gene="pdxI"
                     /locus_tag="ACHOUK_000009"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HFP9895547.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIEALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEKV
                     LSTLDGISRE"
     gene            complement(13207..15666)
                     /locus_tag="ACHOUK_000010"
     CDS             complement(13207..15666)
                     /locus_tag="ACHOUK_000010"
                     /inference="COORDINATES: protein motif:HMM:NF015737.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFP9895548.1"
                     /translation="MNEGGALTNEGVLNTVTLEGGTFNNNGTLNDVVKIEKNSNAVIN
                     NTGSLSTLQLHDGTVNNSGIASARVNAQGDAVFNNLAGGEARKGAILYNSAVVNNAGT
                     WKMGYQDENNNAGTLDIDDKSTFNNSGKLILDNSKNAIRFQGSNANATLYNTGEMTLD
                     AALGAGAIHYDDGASEFINKGVVDAKVTVAVSTAGATESDAFLWNQDGGVINFDKDNA
                     SAVKFTHNNYVALNDGVMNISGNNAVAMEGDKNAQLVNNGVINLGTEGTTDTGLTGMQ
                     LDANATADAVIENNGTINIFANDSFAFSVLGTEGHIVNNGTVVIADGVTGSGLIKQGD
                     SVNVEGVNGNSGNNTEVHYTDYTLPDMPNTYTTSPFSETTDSGSSDGSSNNLNGYIVG
                     TNVDGSAGKLKVNNASMNGVGINTGFAAGTADTTVSFDNVVEGINLTDADAITSTSVV
                     WTAKGSTDASGNVDVIMSKNAYTDVATDASVNDVAKALDAGYTNNELYTSLNVGTTAE
                     LNSALKQVSGSQATTVFREARVLSNRFSMLADAAPKVGNGLAFNVVAKGDPRAELGNN
                     TEYDMLALRKTVDLSESQSMSLEYGIARLDGDGAQKAGDNGVTGGYSQFFGLKHQMSF
                     DNGMRWNNALRYDVHNLDSSRSVAYGDVSKTADTDVKQQYLELRSEGAKTFEPREGLK
                     ITPYAGVKLRHSLEGGYQERNAGDFNLSMNSGSETAVDSIVGLKLDYAGKGGWSANAT
                     LEGGPNLSYSKSQRTASLAGAGSQHFNVDDGQKGGGINSLASVGVKYSSKESSLNLDA
                     YHWKEDGISDKGVMLNFKKTF"
     gene            complement(15886..18921)
                     /locus_tag="ACHOUK_000011"
     CDS             complement(15886..18921)
                     /locus_tag="ACHOUK_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed virulence protein BigA"
                     /protein_id="HFP9895549.1"
                     /translation="MQRKKLLSVCVAMALSSQTWAADTSIPDSTEKTRKSSKITCPAN
                     IHSLSKEQLENLSAECRENKDSAVLPWAAAGLAAVATGIAIYTLSDDDNHHHNNSPVP
                     DDGGDTPVPPDDGGDTPVPDDGGDTPVPPDDGGDTPVPPDDGGDTPVPPDDGGDTPVP
                     PDDGGDTPVKHNPVVYKNGVTWDQDAKTVKIRETTFTYSQNADGSYTLTAPEGKETIV
                     KSWTVHDDSNTVVFDGVNTSGGITWSYDDDGLIHITKEAGVVVDGTTGNAIEFGNAII
                     TDQGGNTALNGGTVMTVDGDNISLNNDGKTTAIGEGSVVGILTGDNITINNNGETEVD
                     GGTAVIINGDNTKLNTAGDSTITNGGTGSLINGDNARVDNQGTMSVDGENSTGSKIVG
                     DGATIKQEGDLYVSGGAHGIDVDGNDTFVSNKGNITVIEDNSIGMLLDGDGVSVINMG
                     DLNVGQAAAGENAIGIQIDGDNATFVNVGDISATNAGTGVSVAGDKANISLAGGLDVG
                     DFSTGLDVSGNNNNMTLATYELNVTGQKATGVNVSGDGNTIEIAGSILVDKDQKADNA
                     QPYFFNPSTGVNISGDNNDVTLDGQLTVVADSKTTSRSYASYDGAQEHIAGIVIAGDD
                     NTFTLNGGVHFVGEKNVMDDGSKPSASRRGIGDTPLINVDGHSPVYLNGESTISGEFP
                     LGFENLIQLSHGAELEIGADATFDMSDVDSFTYYYRVALSTISIDSGAKATNNGEVEL
                     KNIGFAAAWNKDSTVINNGSIGLAMYDFGTDPAPKVFDVEYGGIGVNNGTMTTKMMNQ
                     HSVLNYPAEWNLSDGTSFNNKALGLTGMLASYSSSILNGETGIIDMYGRGSVGMLAID
                     KSTADNEGQITLDTLWVDENDETSLRNNVANSTAKDFGVGMASGTDAYNGALTKATAT
                     NHENGVITVYNAGAGMAAYGNTNTVINQGTINLEKNENYNDSLGVNKLVGMAVYHEGT
                     AINDQTGVININAENGQAFYNDGTGLIINYGTICTFGVC"
     gene            complement(19335..19658)
                     /locus_tag="ACHOUK_000012"
     CDS             complement(19335..19658)
                     /locus_tag="ACHOUK_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFP9895550.1"
                     /translation="MKRTLLIAVWAIGLMSDSAMALTLNEARSQGRVGETLNGYLVAL
                     QTDAETQALVKDINEARNHSYQQLAKQNNVSTKEIAKLAGQKLVARAKSGQYVQGVNG
                     KWLRK"
     gene            complement(19666..19851)
                     /gene="ynbE"
                     /locus_tag="ACHOUK_000013"
     CDS             complement(19666..19851)
                     /gene="ynbE"
                     /locus_tag="ACHOUK_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFP9895551.1"
                     /translation="MKIVIAAFASSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEDLLKTRSDLF"
     gene            complement(19848..22487)
                     /gene="ydbH"
                     /locus_tag="ACHOUK_000014"
     CDS             complement(19848..22487)
                     /gene="ydbH"
                     /locus_tag="ACHOUK_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFP9895552.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPSVKLVGEFTMPLMPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANAGPMTLRLDKDTADAVAKDNMTAGTAINWLRYMEIS
                     RSSTRINLDNLGVLTMQANISGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQV
                     WLEQNAQLPETGCPSGKECEEKQ"
     gene            22695..23684
                     /gene="ldhA"
                     /locus_tag="ACHOUK_000015"
     CDS             22695..23684
                     /gene="ldhA"
                     /locus_tag="ACHOUK_000015"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFP9895553.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            23795..24217
                     /gene="hslJ"
                     /locus_tag="ACHOUK_000016"
     CDS             23795..24217
                     /gene="hslJ"
                     /locus_tag="ACHOUK_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFP9895554.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTCDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(24214..24480)
                     /gene="ydbJ"
                     /locus_tag="ACHOUK_000017"
     CDS             complement(24214..24480)
                     /gene="ydbJ"
                     /locus_tag="ACHOUK_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFP9895555.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            24754..28278
                     /gene="nifJ"
                     /locus_tag="ACHOUK_000018"
     CDS             24754..28278
                     /gene="nifJ"
                     /locus_tag="ACHOUK_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFP9895556.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPAFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            28644..29757
                     /gene="ompN"
                     /locus_tag="ACHOUK_000019"
                     /pseudo
     CDS             28644..29757
                     /gene="ompN"
                     /locus_tag="ACHOUK_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            29898..30332
                     /gene="uspF"
                     /locus_tag="ACHOUK_000020"
     CDS             29898..30332
                     /gene="uspF"
                     /locus_tag="ACHOUK_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFP9895557.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            30508..31443
                     /gene="ttcA"
                     /locus_tag="ACHOUK_000021"
     CDS             30508..31443
                     /gene="ttcA"
                     /locus_tag="ACHOUK_000021"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFP9895558.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(31572..32945)
                     /gene="dbpA"
                     /locus_tag="ACHOUK_000022"
     CDS             complement(31572..32945)
                     /gene="dbpA"
                     /locus_tag="ACHOUK_000022"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFP9895559.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(32978..33148)
                     /gene="ynaL"
                     /locus_tag="ACHOUK_000023"
     CDS             complement(32978..33148)
                     /gene="ynaL"
                     /locus_tag="ACHOUK_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFP9895560.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRA"
     gene            complement(33423..34406)
                     /gene="zntB"
                     /locus_tag="ACHOUK_000024"
     CDS             complement(33423..34406)
                     /gene="zntB"
                     /locus_tag="ACHOUK_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFP9895561.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(34420..34467)
                     /gene="ydaN"
                     /locus_tag="ACHOUK_000025"
     CDS             complement(34420..34467)
                     /gene="ydaN"
                     /locus_tag="ACHOUK_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFP9895562.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            34661..35893
                     /gene="dgcM"
                     /locus_tag="ACHOUK_000026"
     CDS             34661..35893
                     /gene="dgcM"
                     /locus_tag="ACHOUK_000026"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFP9895563.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFIHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(35914..36477)
                     /gene="smrA"
                     /locus_tag="ACHOUK_000027"
     CDS             complement(35914..36477)
                     /gene="smrA"
                     /locus_tag="ACHOUK_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFP9895564.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(36807..37715)
                     /gene="abgR"
                     /locus_tag="ACHOUK_000028"
     CDS             complement(36807..37715)
                     /gene="abgR"
                     /locus_tag="ACHOUK_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9895565.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            37891..39201
                     /gene="abgA"
                     /locus_tag="ACHOUK_000029"
     CDS             37891..39201
                     /gene="abgA"
                     /locus_tag="ACHOUK_000029"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFP9895566.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNENSRMGLPDELTLQREFERARQQGALAQWIAAFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTTIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGYNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            39201..40646
                     /gene="abgB"
                     /locus_tag="ACHOUK_000030"
     CDS             39201..40646
                     /gene="abgB"
                     /locus_tag="ACHOUK_000030"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFP9895567.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSVTP
                     GENGHGCGHNLLGTAAFAAAIAIKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYQALSHFGTPEWNSEELAFAKQIQ
                     ATLTSNDRQNSLNNIAATGGENGKVFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTLNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            40683..42209
                     /gene="abgT"
                     /locus_tag="ACHOUK_000031"
     CDS             40683..42209
                     /gene="abgT"
                     /locus_tag="ACHOUK_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HFP9895568.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            42220..42735
                     /gene="ogt"
                     /locus_tag="ACHOUK_000032"
     CDS             42220..42735
                     /gene="ogt"
                     /locus_tag="ACHOUK_000032"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFP9895569.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGELAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            42930..43682
                     /gene="fnr"
                     /locus_tag="ACHOUK_000033"
     CDS             42930..43682
                     /gene="fnr"
                     /locus_tag="ACHOUK_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFP9895570.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            43834..44784
                     /gene="uspE"
                     /locus_tag="ACHOUK_000034"
     CDS             43834..44784
                     /gene="uspE"
                     /locus_tag="ACHOUK_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFP9895571.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(44834..45091)
                     /gene="ynaJ"
                     /locus_tag="ACHOUK_000035"
     CDS             complement(44834..45091)
                     /gene="ynaJ"
                     /locus_tag="ACHOUK_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFP9895572.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            45335..46366
                     /gene="ynaI"
                     /locus_tag="ACHOUK_000036"
     CDS             45335..46366
                     /gene="ynaI"
                     /locus_tag="ACHOUK_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFP9895573.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            complement(46417..48030)
                     /gene="mppA"
                     /locus_tag="ACHOUK_000037"
     CDS             complement(46417..48030)
                     /gene="mppA"
                     /locus_tag="ACHOUK_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFP9895574.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDVHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(48366..49265)
                     /gene="pgrR"
                     /locus_tag="ACHOUK_000038"
     CDS             complement(48366..49265)
                     /gene="pgrR"
                     /locus_tag="ACHOUK_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFP9895575.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLIIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            49403..50323
                     /locus_tag="ACHOUK_000039"
     CDS             49403..50323
                     /locus_tag="ACHOUK_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9895576.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAIMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPTQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            50323..51253
                     /locus_tag="ACHOUK_000040"
                     /pseudo
     CDS             50323..51253
                     /locus_tag="ACHOUK_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622627.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            51274..51912
                     /locus_tag="ACHOUK_000041"
     CDS             51274..51912
                     /locus_tag="ACHOUK_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309933.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9895577.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSPKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            52203..52931
                     /gene="mpaA"
                     /locus_tag="ACHOUK_000042"
     CDS             52203..52931
                     /gene="mpaA"
                     /locus_tag="ACHOUK_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFP9895578.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(52906..53871)
                     /gene="ycjG"
                     /locus_tag="ACHOUK_000043"
     CDS             complement(52906..53871)
                     /gene="ycjG"
                     /locus_tag="ACHOUK_000043"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFP9895579.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDTTLIVDANESWHAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            53990..54496
                     /gene="tpx"
                     /locus_tag="ACHOUK_000044"
     CDS             53990..54496
                     /gene="tpx"
                     /locus_tag="ACHOUK_000044"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFP9895580.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(54540..56081)
                     /gene="tyrR"
                     /locus_tag="ACHOUK_000045"
     CDS             complement(54540..56081)
                     /gene="tyrR"
                     /locus_tag="ACHOUK_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFP9895581.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRASKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(56229..57290)
                     /gene="ycjF"
                     /locus_tag="ACHOUK_000046"
     CDS             complement(56229..57290)
                     /gene="ycjF"
                     /locus_tag="ACHOUK_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFP9895582.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(57287..58684)
                     /gene="ycjX"
                     /locus_tag="ACHOUK_000047"
     CDS             complement(57287..58684)
                     /gene="ycjX"
                     /locus_tag="ACHOUK_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFP9895583.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRYKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            58839..59837
                     /gene="ycjW"
                     /locus_tag="ACHOUK_000048"
     CDS             58839..59837
                     /gene="ycjW"
                     /locus_tag="ACHOUK_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9895584.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHTPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLEDHNIAVRD
                     EWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGRQSCELLFRLIAGKPSPQNITVATHMTLK"
     gene            complement(59948..60853)
                     /gene="ompG"
                     /locus_tag="ACHOUK_000049"
     CDS             complement(59948..60853)
                     /gene="ompG"
                     /locus_tag="ACHOUK_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFP9895585.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRISLAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSISLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(60898..61980)
                     /locus_tag="ACHOUK_000050"
     CDS             complement(60898..61980)
                     /locus_tag="ACHOUK_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9895586.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQESLH
                     KPIVMGMRPEDIHPDAQEENNISAKISVVELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(61994..62653)
                     /gene="pgmB"
                     /locus_tag="ACHOUK_000051"
     CDS             complement(61994..62653)
                     /gene="pgmB"
                     /locus_tag="ACHOUK_000051"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFP9895587.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERTQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWPRLSAFWQN
                     V"
     gene            complement(62650..64917)
                     /gene="ycjT"
                     /locus_tag="ACHOUK_000052"
     CDS             complement(62650..64917)
                     /gene="ycjT"
                     /locus_tag="ACHOUK_000052"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFP9895588.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSHEAWQRELDFASGELR
                     RNVVWRTSNGAGYTIASRRFVSADQLPLIALEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEICAQQSYDQLLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRSGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMARYNVQQALNIARQFGCSDDA
                     FIHRAEMFLKELWMPETQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAVSCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(64914..65969)
                     /gene="ycjS"
                     /locus_tag="ACHOUK_000053"
     CDS             complement(64914..65969)
                     /gene="ycjS"
                     /locus_tag="ACHOUK_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9895589.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKQGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGYPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLGSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(65979..66767)
                     /gene="ycjR"
                     /locus_tag="ACHOUK_000054"
     CDS             complement(65979..66767)
                     /gene="ycjR"
                     /locus_tag="ACHOUK_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFP9895590.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNIEEV
                     KSAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYSVENNLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLTWLRTC"
     gene            complement(66785..67837)
                     /gene="ycjQ"
                     /locus_tag="ACHOUK_000055"
     CDS             complement(66785..67837)
                     /gene="ycjQ"
                     /locus_tag="ACHOUK_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFP9895591.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETIIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWKLLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(67868..68710)
                     /gene="ycjP"
                     /locus_tag="ACHOUK_000056"
     CDS             complement(67868..68710)
                     /gene="ycjP"
                     /locus_tag="ACHOUK_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9895592.1"
                     /translation="MATNKRTLSRIGFYRGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPVIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(68697..69578)
                     /gene="ycjO"
                     /locus_tag="ACHOUK_000057"
     CDS             complement(68697..69578)
                     /gene="ycjO"
                     /locus_tag="ACHOUK_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9895593.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVIFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(69599..70891)
                     /gene="ycjN"
                     /locus_tag="ACHOUK_000058"
                     /pseudo
     CDS             complement(69599..70891)
                     /gene="ycjN"
                     /locus_tag="ACHOUK_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(70905..72583)
                     /gene="ycjM"
                     /locus_tag="ACHOUK_000059"
                     /pseudo
     CDS             complement(70905..72583)
                     /gene="ycjM"
                     /locus_tag="ACHOUK_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
     gene            complement(72793..73107)
                     /gene="pspE"
                     /locus_tag="ACHOUK_000060"
     CDS             complement(72793..73107)
                     /gene="pspE"
                     /locus_tag="ACHOUK_000060"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFP9895594.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATVVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(73182..73403)
                     /gene="pspD"
                     /locus_tag="ACHOUK_000061"
     CDS             complement(73182..73403)
                     /gene="pspD"
                     /locus_tag="ACHOUK_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFP9895595.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(73412..73771)
                     /gene="pspC"
                     /locus_tag="ACHOUK_000062"
     CDS             complement(73412..73771)
                     /gene="pspC"
                     /locus_tag="ACHOUK_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFP9895596.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(73771..73995)
                     /gene="pspB"
                     /locus_tag="ACHOUK_000063"
     CDS             complement(73771..73995)
                     /gene="pspB"
                     /locus_tag="ACHOUK_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFP9895597.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(74049..74717)
                     /gene="pspA"
                     /locus_tag="ACHOUK_000064"
     CDS             complement(74049..74717)
                     /gene="pspA"
                     /locus_tag="ACHOUK_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFP9895598.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            74884..75861
                     /gene="pspF"
                     /locus_tag="ACHOUK_000065"
     CDS             74884..75861
                     /gene="pspF"
                     /locus_tag="ACHOUK_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFP9895599.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(75992..77257)
                     /gene="puuE"
                     /locus_tag="ACHOUK_000066"
     CDS             complement(75992..77257)
                     /gene="puuE"
                     /locus_tag="ACHOUK_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFP9895600.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERAMQLGQRLTKTL
                     IDAKENVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALRD"
     gene            complement(77295..78575)
                     /gene="puuB"
                     /locus_tag="ACHOUK_000067"
     CDS             complement(77295..78575)
                     /gene="puuB"
                     /locus_tag="ACHOUK_000067"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFP9895601.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLEMLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTATGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSDDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYDARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(78577..80064)
                     /gene="puuC"
                     /locus_tag="ACHOUK_000068"
     CDS             complement(78577..80064)
                     /gene="puuC"
                     /locus_tag="ACHOUK_000068"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFP9895602.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLANIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLIEANAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVVLKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQQAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIQEGESKGQLLLDGRNAGLAVAIGPTIIVDVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(80214..80771)
                     /gene="puuR"
                     /locus_tag="ACHOUK_000069"
     CDS             complement(80214..80771)
                     /gene="puuR"
                     /locus_tag="ACHOUK_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFP9895603.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIILTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(80798..81562)
                     /gene="puuD"
                     /locus_tag="ACHOUK_000070"
     CDS             complement(80798..81562)
                     /gene="puuD"
                     /locus_tag="ACHOUK_000070"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFP9895604.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPERDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            81774..83192
                     /gene="puuA"
                     /locus_tag="ACHOUK_000071"
     CDS             81774..83192
                     /gene="puuA"
                     /locus_tag="ACHOUK_000071"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFP9895605.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            83341..83505
                     /locus_tag="ACHOUK_000072"
     CDS             83341..83505
                     /locus_tag="ACHOUK_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9895606.1"
                     /translation="MPMIKSPHGAGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            83495..84877
                     /gene="puuP"
                     /locus_tag="ACHOUK_000073"
     CDS             83495..84877
                     /gene="puuP"
                     /locus_tag="ACHOUK_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFP9895607.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVAMSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRKGMNK
                     SWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPLY
                     EGDRTPVSET"
     gene            85011..85256
                     /gene="ymjA"
                     /locus_tag="ACHOUK_000074"
     CDS             85011..85256
                     /gene="ymjA"
                     /locus_tag="ACHOUK_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2543 family protein"
                     /protein_id="HFP9895608.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            85569..87212
                     /gene="sapA"
                     /locus_tag="ACHOUK_000075"
     CDS             85569..87212
                     /gene="sapA"
                     /locus_tag="ACHOUK_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFP9895609.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTAWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKLLGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKTLSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            87209..88174
                     /gene="sapB"
                     /locus_tag="ACHOUK_000076"
     CDS             87209..88174
                     /gene="sapB"
                     /locus_tag="ACHOUK_000076"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFP9895610.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            88161..89051
                     /gene="sapC"
                     /locus_tag="ACHOUK_000077"
     CDS             88161..89051
                     /gene="sapC"
                     /locus_tag="ACHOUK_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFP9895611.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCM
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            89051..90043
                     /gene="sapD"
                     /locus_tag="ACHOUK_000078"
     CDS             89051..90043
                     /gene="sapD"
                     /locus_tag="ACHOUK_000078"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFP9895612.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            90045..90851
                     /gene="sapF"
                     /locus_tag="ACHOUK_000079"
     CDS             90045..90851
                     /gene="sapF"
                     /locus_tag="ACHOUK_000079"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFP9895613.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTNLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(90903..92066)
                     /locus_tag="ACHOUK_000080"
     CDS             complement(90903..92066)
                     /locus_tag="ACHOUK_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HFP9895614.1"
                     /translation="MARVSLSWALILGLLAGIGPMCTDLYLPALPEMSEQLAATTTIT
                     QLTLTASLIGLGVGQLLFGPLSDKIGRKRPLILSLLLFIVSSILCATTNNIYWLVVWR
                     FIQGIAGAGGSVLSRSIARDKYQGVTLTQFFALLMTVNGLAPVLSPVLGGYIVSTFDW
                     RTLFWVMAEISTVLLLGCVLFINETLPENKRGSSLLLTGRSVVQNRRFMRFCLIQSFM
                     LAGLFAYIGSSSFVLQKEFGFSPMQFSLVFGLNGIGLIIASWIFSRLARRINAMTLLR
                     GGLIAAILCALLTVLCAWTQLPIPALVALFFTIAFCSGIGTVGGAEAMSAVGTQESGT
                     ASALMGMSMFVFGGIAAPLSGFGGETLLKMSLAITVCYTLAFLVALTRIDNQK"
     gene            complement(92075..93448)
                     /locus_tag="ACHOUK_000081"
     CDS             complement(92075..93448)
                     /locus_tag="ACHOUK_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HFP9895615.1"
                     /translation="MLRRSLIFLVLLSAGCVSLDPHYSTPESPIPATLPGAQGQGKAI
                     SHDWQQVIHDPRLQQVVTIALNSNRDVQKAIADIDSARALYGQTNASLFPTVNAALSS
                     TRSRSLANGTETTAEADGTVSSFTLDLFGRNQSLSRAARETWLASEFTAQNTRLTLIA
                     EISTAWLTLAADNSNLALAKETMTSAENSLKIIQRQQQVGTAAATDVSEAMSVYQQAR
                     ASVASYQTQVMQDKNALNLLAGTTLEENLLPGTLESLPEQMISLVPAGVSSDVLLRRP
                     DIQEAEHNLKSANADIGAARANFFPTISLTASAGVGSDALSSLFSHGMQIWSFTPSVT
                     LPLFTGGSNLAQLRYAEAQKRGLIATYEKTVQRAFKDVANALARRTTLEEQLDAQRQY
                     VKAEQQTVDVGLRRYQTGVGDYLTVLTAQRSLWSAQQELLALQLTDFTNRITLWQSLG
                     GGMSSLK"
     gene            complement(93452..96560)
                     /locus_tag="ACHOUK_000082"
                     /pseudo
     CDS             complement(93452..96560)
                     /locus_tag="ACHOUK_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133655.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
     gene            complement(96560..97681)
                     /locus_tag="ACHOUK_000083"
     CDS             complement(96560..97681)
                     /locus_tag="ACHOUK_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFP9895616.1"
                     /translation="MKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTVKSQPVSV
                     VSELTGRTSAALSAEVRPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEARA
                     ALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVAAKKAALETAR
                     INLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRL
                     RKQSLATNSDTMSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLL
                     PGMFVRARVDEGVMEDAILAPQQGVTRDAKGNATALVVNKDNKVEQRTLETGETYGDK
                     WLVLNGLHSGDRLIVEGSAKVTSGQTVKAVEVQANGGNA"
     gene            complement(97830..98396)
                     /locus_tag="ACHOUK_000084"
     CDS             complement(97830..98396)
                     /locus_tag="ACHOUK_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9895617.1"
                     /translation="MTSKLEIRHKQRQDEIINAARRCFRRCGFHAASMSQIASEAQLS
                     VGQIYRYFANKDAIIEEMVRRIIDFRIAQMDIDARTDHFPEVLALRKSLNEDDDALML
                     EVAAEATRNPRVMAMLEEADARMFANGCAHMKRMHPHLSEEHIRCCVEVFATMMEGTV
                     YRRLTPQKSDPQHLQEIYQDIVSMLINK"
     gene            98874..99662
                     /gene="fabI"
                     /locus_tag="ACHOUK_000085"
     CDS             98874..99662
                     /gene="fabI"
                     /locus_tag="ACHOUK_000085"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFP9895618.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            99806..100933
                     /gene="yciW"
                     /locus_tag="ACHOUK_000086"
     CDS             99806..100933
                     /gene="yciW"
                     /locus_tag="ACHOUK_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFP9895619.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LEKETLAPFLSWLDPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSTRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESLRWRSSYEV
                     EKLPEEHTKSSTAELCQLAEILSLHPISLSLLERLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            101001..102935
                     /gene="rnb"
                     /locus_tag="ACHOUK_000087"
     CDS             101001..102935
                     /gene="rnb"
                     /locus_tag="ACHOUK_000087"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFP9895620.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVRKDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(103054..103116)
                     /locus_tag="ACHOUK_000088"
                     /pseudo
     CDS             complement(103054..103116)
                     /locus_tag="ACHOUK_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            103170..105155
                     /gene="pdeR"
                     /locus_tag="ACHOUK_000089"
     CDS             103170..105155
                     /gene="pdeR"
                     /locus_tag="ACHOUK_000089"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFP9895621.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPERTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLNEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQELIDHAINQADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSAAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTAY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            complement(105137..105265)
                     /gene="ymiD"
                     /locus_tag="ACHOUK_000090"
     CDS             complement(105137..105265)
                     /gene="ymiD"
                     /locus_tag="ACHOUK_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFP9895622.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTEYSAKYIYSSTYARFR"
     gene            105303..105476
                     /gene="yciZ"
                     /locus_tag="ACHOUK_000091"
     CDS             105303..105476
                     /gene="yciZ"
                     /locus_tag="ACHOUK_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFP9895623.1"
                     /translation="MSEFDAQRVAERIDIVLDILVADDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            105566..106315
                     /gene="yciT"
                     /locus_tag="ACHOUK_000092"
     CDS             105566..106315
                     /gene="yciT"
                     /locus_tag="ACHOUK_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFP9895624.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            106584..106802
                     /gene="osmB"
                     /locus_tag="ACHOUK_000093"
     CDS             106584..106802
                     /gene="osmB"
                     /locus_tag="ACHOUK_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFP9895625.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(106928..107254)
                     /gene="yciH"
                     /locus_tag="ACHOUK_000094"
     CDS             complement(106928..107254)
                     /gene="yciH"
                     /locus_tag="ACHOUK_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFP9895626.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRSKGDGVVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(107254..107991)
                     /gene="pyrF"
                     /locus_tag="ACHOUK_000095"
     CDS             complement(107254..107991)
                     /gene="pyrF"
                     /locus_tag="ACHOUK_000095"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFP9895627.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDAALAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(108184..109353)
                     /gene="lapB"
                     /locus_tag="ACHOUK_000096"
     CDS             complement(108184..109353)
                     /gene="lapB"
                     /locus_tag="ACHOUK_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFP9895628.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(109360..109668)
                     /gene="lapA"
                     /locus_tag="ACHOUK_000097"
     CDS             complement(109360..109668)
                     /gene="lapA"
                     /locus_tag="ACHOUK_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFP9895629.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLVRAERKIKRLENQLSPATDVAVVQHSSAAKE
                     "
     gene            complement(109817..110581)
                     /gene="pgpB"
                     /locus_tag="ACHOUK_000098"
     CDS             complement(109817..110581)
                     /gene="pgpB"
                     /locus_tag="ACHOUK_000098"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFP9895630.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            110751..111341
                     /gene="ribA"
                     /locus_tag="ACHOUK_000099"
     CDS             110751..111341
                     /gene="ribA"
                     /locus_tag="ACHOUK_000099"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFP9895631.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(111405..114080)
                     /gene="acnA"
                     /locus_tag="ACHOUK_000100"
     CDS             complement(111405..114080)
                     /gene="acnA"
                     /locus_tag="ACHOUK_000100"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFP9895632.1"
                     /translation="MSSTLREASKDALQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLDLDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPIYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VARSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(114244..114339)
                     /gene="ymiC"
                     /locus_tag="ACHOUK_000101"
     CDS             complement(114244..114339)
                     /gene="ymiC"
                     /locus_tag="ACHOUK_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFP9895633.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(114453..114620)
                     /gene="yciX"
                     /locus_tag="ACHOUK_000102"
     CDS             complement(114453..114620)
                     /gene="yciX"
                     /locus_tag="ACHOUK_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFP9895634.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(114623..114763)
                     /gene="ymiA"
                     /locus_tag="ACHOUK_000103"
     CDS             complement(114623..114763)
                     /gene="ymiA"
                     /locus_tag="ACHOUK_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFP9895635.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
BASE COUNT        28491 a        30111 c        27723 g        28667 t
ORIGIN      
        1 tctggaagtt tttcatagaa agatattaaa gcctgaccaa cgcgatcacc ctctattatc
       61 cgttcaaatt gcgacctcag taaaatatca tgtctactca ttgcaatacg tcctgttcgc
      121 ccaaggcgaa gtatgaatga aacttatgta aaatagtacc gcccttgcta tatttcagaa
      181 ttcataaaga aaaaacccgg cgctaaatgc ttaccgggtt ttagatttat caggcaaata
      241 gcagggttaa ccgctaatct gctccatcgc ctgcaaaata cgcttatctg aaatcggata
      301 aggcgtacca agttgttgag cgaagtaact aacgcgcagc tcttctatca tccaacggat
      361 ctctttcacg tcttcatcct cacgacgtgc aggcgggagt ttgttgaacc attgctgcca
      421 cgcctgctgg acgttttcga ctttcagcat ctgtgcacga tcgcgatgtg gatcaaccgc
      481 cagtttttcc agccgttttt caatcgcctg caaataacgc agcgtgtcgc ccagccgttt
      541 gaagccgtta ccagtgacaa aaccgcgata taccaacccg cccatctgcg ctttaatgtc
      601 agaaagcccc agcgccatgg tcatatccac ccgccctttc agacgtttgt tgatattgaa
      661 cactgccgta aggatttgct cgacctgctt cgcaatatcc accaccgtgt cgttcagttc
      721 ggcacgcact ttttcatgca gcgcagcaaa gccttcttcc gtccagaccg ggcctccatt
      781 ggcgtcgatc agttgatcca caccgcatga gatacagtcg tcgatcagct ccagcacttt
      841 gccatacggg ttaaagtaca gccccagctt ggctttgttt ggtaacttct catgtaaata
      901 cttgattggt gagggaatat tcagcagcag tagacggcga agaccgttcc acattgcctg
      961 tttttgctcc agcgggttat caaacagttt gatcgccaca ctgtcgcgct catccaccag
     1021 cgccggccac gccttcactt tgtagttacc acgcttctgt tcgtagcttt ccggcaactg
     1081 accaaaactc cagatatgta agccgctctg ctcgataccg tcatccgcca ccgcagatag
     1141 cgtttcctgc actttgcctt tcagcgcatc tttcagatct tgtagcgagc gcccttcttt
     1201 tagcttcttg tttttgtcat ccaccacgcg gaaggtgatt ttcaggtgat cgggcacctg
     1261 atcccagtgc cagtcttcgc ggtcaacggt aacgccggtc atccgccgta actcgcgctc
     1321 aaggctgtcg agcaacggta actccagcgg tgtgacgcgg cctaaaaacg cttcggcata
     1381 gtttggcgca ggtacaaaat tacggcgtac cggtttcggc aacgatttaa tcagagcaat
     1441 caccagttcg cggcgcagac cagggatctg ccattcaaac ccgctttcct caacctggtt
     1501 aagtaacggc agcggaatat gtacggtcac accgtcagca tccgcgccgg gctcaaactg
     1561 atagctcaaa cgcagcttga gattgccctg atgccagaag ttcgggtaat ccagcttgct
     1621 gatcttttcc gccccttctt tgatcaacat gctcttttca aagttgagca aatcaggcgt
     1681 ttcgcggctg acttttttcc accagctgtc gaagtgacga gcggagatta catcgtggcc
     1741 gatgcgctgg tcgtagaact caaacagcgt ttcgtcatca accagaatat cgcggcgacg
     1801 tgatttgtgt tcaagctctt ccacttcggc ccgcagtttc aggttttcac ggaagaatgc
     1861 gtgacgcgtc tgccagtcac cttccaccag cgcgtggcga ataaagagtt cacgacataa
     1921 cgccggatcg atctggctgt agttgacctt gcgcgcggca acaatcggca aaccataaac
     1981 agtgactttt tccgttgcca tcaccgcgcc ctgcgcccgt tcccagtgcg gttcgctgta
     2041 ggtacgttta atcaaatgct gagcgacagg ctccacccat tccgggtcga tacgcgcagc
     2101 aatgcgcccc cacaggcggc tggtttctac cagttccgcc accattaccc atttcggcgg
     2161 ttttttgaat aaaccagaac cggggaagat ggagaaacgc gcgttacgtg cgccggtata
     2221 ttcttgttta tcggcatctt tcatgccgat atgggaaagc aaccccgtca gcaacgcaat
     2281 gtgaatttca cgatactccg ccggttcgct gttcaccgga atgccgagtt ctttcaccac
     2341 ctggcgcaac tgggtgtaga tatcctgcca ttcgcgcaca cgcagatagt tgagataatc
     2401 ggtacgacac aggcgacgga aggcgttgga agaaagcgcc ttttgctgct cgccaagata
     2461 attccacaga ttcacaaacg cgaggaagtc agactctttg tcgtggaagc gacgatgttt
     2521 ttcgtccgat gcctgctgtt tgtccatcgg acgttcgcgc ggatcctgaa tggagagcgc
     2581 agacgtgata atcatcgcct cacgcacgca gccatgtttt tgcgcttcca gcaccatacg
     2641 cgccagacgt gggtcgacag gcaactgcga gagctggcga ccgagcggcg tcagtttata
     2701 ggcgctggcc tgttcatccg tggtgatcgc gcccagttct tcgagcagac gcacgccatc
     2761 ctggatattg cgtttatccg gtgcttcgac aaacgggaac gcagcgatat cgcctagccc
     2821 cagcgcggtc atctgcaaaa taaccgaggc caggttggta cgcagaatct ccggatcggt
     2881 aaactccggg cgcgagagga aatcgtcttc ggaatagaga cgaatacaga tcccttcgga
     2941 cacacgacca cagcggcctt tacgctgatt ggcagacgcc tgcgaaatgg gctcaatcgg
     3001 caaacgctgc actttggtgc gatagctgta gcggctgata cgcgccgtac cggggtcgat
     3061 aacgtattta atccccggta cagtcagcga ggtttccgcg acgttggtcg ccagcacaat
     3121 gcgtcgcccg ctgtgcgact ggaacacgcg gttctgttcg ctattcgaaa gccgcgcata
     3181 aagcggcaag atttcggtat ggcgtaagtt cagcttgttc agcgcatcgg cggtatcgcg
     3241 aatttcccgc tcgccgctca taaagatcag aatgtcgccg gggctttcct ggcttagttc
     3301 gtccacggca tcgaaaattg cctgcaactg gtcgcgccca gtgtcgtcgg cttcttcaac
     3361 aatcgggcga tagcgcactt ccaccggata ggtccgaccg gagacttcaa taatcggcgc
     3421 attattaaag tggcgcgaaa agcgttccgg gtcgatagtc gcggaagtga taatgatttt
     3481 taggtcagga cgccgcggca gcaactcttt caaataaccg agcagaaaat cgatattcag
     3541 gctacgttcg tgcgcttcgt caataatgat agtgtcgtac tgcatcagca ggcggtcttg
     3601 ctggatctcc gccagcagga taccgtcggt cattagcttg accatcgtgt tatcacttac
     3661 gtgatcgctg aaacgcactt tgtaaccgat gcaaccgccc ggctccgttt tcagctcttc
     3721 cgcaatacgg ttcgctaccg ttcttgccgc caggcgacgc ggctgggtat ggccgatcag
     3781 ccctttaatc ccgcgcccca gctccataca gattttcggt aactgagtcg ttttaccaga
     3841 acccgtttcc ccggcgacta tcaccacctg gtgatcacga atcgcttcga gaatgtcctg
     3901 ttttttctga ctaaccggca aattgtcagg ataagtaatt tccggtcgtg ccgcttcacg
     3961 cagcaggact ttccctgccg cctggtcaat ctctttcgcc atctcctgga aaatggcctg
     4021 ttgtgcatca ggatttttaa ccttcttcac gccgtgcaga cggcgagaaa aacgcagtct
     4081 gtcacgcagc atcagcgaat ccagccgctg ctgcaaggcc gtaaaagtca atttttgttg
     4141 ttctgtcata acgttagtgg gcagtagtct gcctgattaa ttttctttga aatgatagat
     4201 atagagtacc acatcgcggc tttctatgcg ttgttcaata aattcgaaca taggcttcga
     4261 tttattgcgc tatctctgtg gcaggattgt gaataaagtg tcaacaagca acggggcatc
     4321 gcccattcaa atatctataa ggaaacacca tgagcaaggt attagttctt aaatccagca
     4381 tcctggcagg gtactctcag tctaatcagt tgtccgatta ttttgttgaa caatggcgcg
     4441 aaaagcactc cgctgatgaa atcaccgttc gcgacctggc tgcaaatcca attccggtac
     4501 tggatggcga actggttggc gctctgcgtc cgagcgatgc gccgctgact acgcgtcagc
     4561 aggaagctct ggcactttcc gatgagctga ttgccgagct gaaagcccat gacgttatcg
     4621 ttattgcggc accgatgtat aacttcaaca tctcgactca gctgaaaaac tattttgacc
     4681 tggttgcacg tgcaggcgtt actttccgct ataccgagaa tggtccggaa ggtctggtaa
     4741 cgggtaaaaa agccatcgtt attaccagcc gcggtgggat ccacaaagat ggaccaacgg
     4801 acctggtgac gccgtatctg tccacgttcc tgggctttat cggcattacc gatgtgaaat
     4861 ttgtcttcgc cgaagggatc gcatacggtc cggaaatggc agcgaaagcg cagtctgacg
     4921 cgaaagcagc catcgacagt attgttgctg cataaccgga cacgatcttg atgtggactg
     4981 tagttcagtc cacatctcaa tccacttacc tgttttccca taatttcaac atcgctttgt
     5041 gctcttcctt aagcacaata tgcgagcgtc tggctcgaat aaagctaatc gcttcatcaa
     5101 ctgttttaca gtgtccgtaa cataacagcc atgccgccac taccagcgca ctgcgcgata
     5161 atcccaatgc gcaatggacc agaacgctgc cttgctcttc gcgtaatgtt tccagcatcg
     5221 ccacggcctg tcggagctcc ccctcttccg gaaccaccag atccagcatc ggtacacaga
     5281 aatagagtcg atcttttgtc gctcgtccgc gagggaattc aaaggtgaca tccagaaccg
     5341 cattctgtgc cggaatatgt cgtggaaacg cccctaaata aacaccagca gtcattttgc
     5401 tcaccggctc caggcgacga caaaaccaac gcatagatag ccacatcccg atgcgccagg
     5461 gcaatgtcag ccagtaaacg gcgggcggga gtttcccctg actatctttc cctgttgtta
     5521 tcgcgccaag cccaccgtac ccacggccaa taatgagtag cgataatact ggccaacata
     5581 accagactga ccaccataac tgaaccatca tcattagctc catcaacaca atgcacgcgc
     5641 acgcgcctac gacatatggc agtgctattt tgattcgacg ttgatcaggt ttctgataat
     5701 tccaacgacg gtcgacgggt atcatccagt caatcaacat acctaccgcc agccctgtga
     5761 tgacatcaat aaaatgatgc tgccaggttg tcagcatcga aatggcgatg agtaaaaacc
     5821 atccgccgca gactttacgc cacctcacag ccagatgctg acgaaagtga cgccaaagta
     5881 gccagcagag aacaatatgc agcgaaggag actggttata aggcagatca aacagttcaa
     5941 gttgcgaaaa tagccatcct gtcaccccac tcacttcagg acggataaaa ctaaacttca
     6001 gcgggtagag aaaaaaaccg cagcaggcca ttaccgttgc cagaataagc cggtggacaa
     6061 gtcggcgctg ttcgaatgtc gagctacaaa cgaacagcga aaatccatat aaaagatcca
     6121 gactccagta agggacaata gtccagggaa gaaaagggat cgccgtttcc cagccgaata
     6181 cctgactggg gatatcatgg ctgttaaagt cctgaaccgc ggtgaactga tttagagatc
     6241 cataggtgaa gaagaaaaac ggggccagca acaataacca gccagcgcct tgtagcaata
     6301 cgttccggcg ttcagttatc acgacgcacc gccatcgaaa ccgtaaaaat gccccactca
     6361 tcaatccgtt gtgtgcattt atcaaatccg gcatcacgca cgagtgaatc catctcccct
     6421 tgcgaacgca cgcgcattac ccacggttta ccatctttat gactggttaa caccccggca
     6481 atcagctcca gttgtgggtg ccacggctgc ccggtgtaga ggagaatgcc gcccggttcg
     6541 atggcattgg caagacctgc gagtgagttt tttacctgct catttccggg aaaaagctca
     6601 tacagtccag agacaatcgc cagcgtaggc cgcggagtta acgcgctgag ttcctccggg
     6661 ttaaacgcat cgccctgttc aaaacgcacc cgcccagaca ttccccgttg agcaatcatc
     6721 tcttgcccct gtgcaacatt taactcgctg taatcacgta acaaaatatc gcttacggca
     6781 ggctcgtttg ccagcgcatc cagtacatag cgcccatgcc ctgcggcaat gtcaaccacg
     6841 cggacggcta aacctttggc gtggagatcg gcaacggctt gtttaatcag tatttgtaaa
     6901 tgggttttgc gctggcgaat accgcgccag ccaacactgt tcaggtagat tttgtcgatt
     6961 aagcgcccga atgcgttact accttgcggt tgattttgat agacataatc cagcgaactg
     7021 cctgaatcaa agccggtgct cattccgaga tgcaggcccg cagagtgcgt cccgaacaat
     7081 ttcatcgctt tacgcataaa gcgatacgcc aaatcaaccg gcgataatgg cacgggtcca
     7141 cctgaaagga gccgccagcg atccgctgat ggtccagtgc ggtcttcatg ctgataatca
     7201 aatttttgtg acttgttagc atataaacga ctaataaagc tttgcatttt ttcaaatgcc
     7261 tgcgccctgt tctcttcacc caacgtgtcg tgataaaagc ctggcagcag atgcagctct
     7321 ttcagagggc tacgtaatct atgataaaaa tcaatctgcg gttggcgatg caccacatag
     7381 tcatcgcctg atatcagaag ttgcgtgggg agcgtaatcg ccgccgcatc gctaacaata
     7441 cgttcagacg ttttataaag atcgagcaag atattaacgg caatcgcccg tgtgatcagc
     7501 ggatcattat tgaaactcgc cacccgttgc cgatcgtggg tcaaatagcg tcctttcaca
     7561 taggaattaa taaaaaacag accacgcaga cgatgccata acgccagcgc aggacgtgcc
     7621 agcggcacat acaatttaac cttaaaggcc ggagaagcca gcaccagccc gcgaattgca
     7681 ggcgcataat catgaaccca tgtggcaacc agcactgcgc cgacgctctg cgcgatcact
     7741 accacctctt ccagcccgac ctggctgtcg ctggcagcaa aacggacaaa ctcatcgaca
     7801 tcccgcactg aacgcgcaag agatgggcta taaccacggg gccccgaagt ttgtccatgc
     7861 cctcgggcat cccatgcata aaaagcagta tctggcatcg ccagttcatc aacgatatgt
     7921 tgtagacgac cagaatgttc atgaccgcga tgaaataaga cgatgacctt tttcgcccct
     7981 ggctgtaaag cgggccagtg ccgataaaac aacgctgtat tatcactggt ggtaaaaaaa
     8041 tgttccccag ggatgcgtga attttccatt ttctctattt tccttcagta acagcagtag
     8101 cgaataaaat aaaaaaatac cggagcggtg aaaattaatg agtcaatcct gtcaaggagt
     8161 ccgccatgtc ctggtaatag ttttccgcta tctttaacac ccacatctcg tttgacggct
     8221 gacatcacta catcgccgca aaaaccacta ataccaatta acaaacccgc taataatgcc
     8281 tgtaatgtat ttagcggtgt cagcaacggt ccaataatca gtgacgcaat catggtggtg
     8341 attacgcccc ccaccagacc ttccagcgtt tttcccgggc tgactttggg gaccactttt
     8401 cttctgccgc aggatttccc ccataaatac tgtgcaatat cgtttgactc tgttaacgcc
     8461 aaaagaaata acaccagtaa cgccccgccc tgggtatttg tggttgtcaa cattaataac
     8521 caggcggcat gactcaaggc gaaaacggtc attatccagc cccaaaaaat ggcgctcacg
     8581 gtatgcagaa aaccggaggt atcacctact aaaactcgcc aggtggttaa tatcagaaac
     8641 ccagccaaag gtataaataa cagaaacagc tcaaagcaac taaaaccgat aagcaaatag
     8701 ttaagaggaa taacccaata taaccaacgc ggaaaatgta cggatataag cgtacaatat
     8761 tctttcaatg ccagaaaact tatcagcgca aaaaacgtca acgtcatcca gcgtggacct
     8821 gaaatcacca gtgaaaaaca aatgacgata ccccaccagg tacgaatacg tagtgtaact
     8881 tcatcccctt ttctctccgg taaccgccat acccgaaaac ggtttatcag cgttgctaaa
     8941 attaacaacg cgaaaagtgt tgccagagat ttttccagca ttatctttca gcactcatca
     9001 ggagcacact ccgacaacga ttaatcgcag tccagagaag cagaattgaa gcaatgctcc
     9061 ataaaagatt attccattgc atccactgtg gataaatggc aacggccagc ccccacaacc
     9121 caaatattaa cgcgcgatcg cttttgccaa aaggaccgac ataactacga ataccgttaa
     9181 tcgtctgcgc gagtaaaccg caaaactcag tcaatatggt gcaaaataac atgagtatta
     9241 cgagtgatgc gttactttcc ggtaaaaata aaaacggtaa atagagcgca atatcggaaa
     9301 taacatcgcc agtttcattt aaaatcgccc ccagacgtgt ttgctgattg cactcacgcg
     9361 ccaacatgcc atccagcgca ttgagtgcca tacggataaa aagcacgatg ggcaatagca
     9421 aaaagaggat gggttgtgcg accaacatca acagcaatcc ggtaataaga gaaagcgcca
     9481 gtgcagcaag agtgatgtga ttcgctgtaa catggtgctt ataaagccaa aacatcgtcg
     9541 gccttaacag cgactgaaag agcggtttta tctggtagag cgtcattatc aatccttgat
     9601 aattcggttt gcgctatggt gcatcaattc ccctgacgga gatatcgcaa cacgacgctt
     9661 cattaaaaat tcagaatata tcaaggtatt ggggactcac tgtcaggaat gattatcccc
     9721 aataaatcaa gtggttaaaa attattacgc tgttattcaa tacaactcat caagttttct
     9781 catcgcttgt tcccgtattg cgcgcgtctt accgttaagc gccagctggc gaactaaatc
     9841 cagttcaggg ttaacaagct ttaacgccag caaaggatca cgtttcagcc tcgcctttaa
     9901 tacatcaact ggaaaacggg gattattcat cgccacaatc caccactcgg gatcaatttc
     9961 tgccgccagt actgcaggcg gtgtgtggcg gttgttaagt gcgccgaact tgaaaaatgt
    10021 gagattgctt ttcgccagtg tactccacca tatacgatcc ggttgttttg ccagtacatc
    10081 cagttgtgct aacgcatcgg ggtctagtgc ataataaaca cgtaacgctt cgttaacctg
    10141 cgcacgggaa ctgttttcct gcctcagaaa aaacttcgcc caaatatcat tgcgtggcgt
    10201 gttttgctgt aattccttga gaacaacagc gggtaaatta tcccaactaa ttaatgcact
    10261 ctctaaaaag tcccagtcgt ccgaacgtat cagagaggtg gcatttaata gcatctggtc
    10321 atcaacttca ccgtagctga taagctcaga caaaatcata tcagcgctgg tttcatatgc
    10381 atctgaatct gctttgggat tatttttccg atgcgataaa gcacgttgcc aaattttttg
    10441 tttcatcgac gtgctcagat actgattttt cgccagatta ctccccccct caggatcgtt
    10501 gaaacgtgtc aataaccgtt gtaaagcagc gtcagagtga attatctgca tatcccgaca
    10561 gcaaaagtag gcaggggatt ttttctccat ccttttaaca aaattaaact gtaagttatc
    10621 tggcaacgct aaaaataacg cacctattag atcatcgtca ttctcgaatt tttggtataa
    10681 atatactgct attttgtcac tctcttgtgg cgtcatccgc aacaattgac catggtgttt
    10741 catctctgcc agctttaacg ctaagcttgt gacaacatcg tttgatgaat cgttcgccaa
    10801 ctgcattgcc tgattgtagg tgagttgtcc tccagcaccg acctgacgaa tagaggaatc
    10861 atcactgcgg atatagccat caataatgga ttcgggcaat aaccacgcat ctttataaag
    10921 ctcctgaatg atcatatccc aatctctttt cgccagagtc atgacgatat ccagagggag
    10981 attttcacgt agcgcaagat tcaacggacc gacaccatca ggtagatgac tcattcgcgt
    11041 cattatttcg acagagagcg gttcacggtc gagcaacccc agaacaacag gctggtaacc
    11101 caattgctga tcggtcaact ccagaatctc attgtcaggc atatatctcg ccatactttc
    11161 aatcgtttgt ccatcattgc tattaataag ttgtttgcgt aggttagctg aaattccttt
    11221 aagccttggg agagttcgca ttatggcaag atcaaatgtc gcaatatctc gtccttcaaa
    11281 tgcagcaacg ttgccttcat ccagcatttg caattcctgc actaactcag gagaaggctt
    11341 aaggcgaaac agcgcttctt ttttcatctg attacagaca ttccctgcca tgcgaatatt
    11401 gcttcgctta aaaatttctt tcagagcatc ttcatcattt attagacccc aataatatgc
    11461 atcaggattt attatcatca gcgctttttt gacattttca gtgtcatttt tgtcactcgc
    11521 acggaccatg aattcactat ttgtcgaaga cgcaacaaca ggtgcaatta tgtcattgat
    11581 aaatatccca cgcgcattaa tactcccctt aatcgtagac ggattatcgt aatatgagtc
    11641 gataaatacc agtccacttg catttatcga accggtaatt aacagtggtg aatccgtaac
    11701 tatattcgtt gccgtcacgt taccgagaat aacccatgga ttagaaagag agaaagtgga
    11761 aatattacct ttgactgtca aattgccaag aacaatcaat ttttgattac tctctatatt
    11821 accttctacc actaaatcgc cacgatgcac tttccagtcc gatgcagata aaccgaaggg
    11881 gacaagattc acgctactgt tgatagcttg ttcaacttgt tgtttatctt cagcggtagc
    11941 cgcagaaaaa aaaggttcaa tatcaactgc atttgcaacg catcgaagtt ccgcctgtac
    12001 tggaagactc agcataaaca ggggaagtaa acctatccag ctgcaaagcc tgggcttaaa
    12061 catatgcact ccatgccatt aaaaaagaaa caaaagggaa atgcatcttt cccttttgta
    12121 tattcgttat tctcgcgaaa taccatccaa cgtagacaac actttctcag aaagatgcaa
    12181 cttttcagca gccatattct cccgtaaatg cgcaacagaa gacgtccctg ggatcagcaa
    12241 aatattcggt gaacgttgta acagccacgc cagcgccacc tgcattggtg ttgcacccaa
    12301 gctcgcagca acatcggaaa gcgtggaaga ttgcagcggc gtaaagcccc cgagcggaaa
    12361 gaacggcacg taggcaatgc cgtcacgagc caaagcttca atcattgcgt catcagcacg
    12421 atgagcaatg ttgtattcgt tctgcacaca gacgatttcg gcaatcttgc gcgcctctgc
    12481 aacctgcgtc ggtgtgacgt tgcttaggcc aatatgtttt accaggcctt gttgttgcat
    12541 ctctgccagc acggtcaggc tggcttcaat cgatccttcc gccgggccat gaccatcccc
    12601 catcatcacg cgcaggttaa ccacatccag tacgtccagg ccgagattac gtagattatc
    12661 gtgcaccgct ttttgcagct ctgccggaga aaatgcgggc aaccaggatg cgtcctctcc
    12721 acgccgcgca ccaattttag tgacaattgt cagatcgtca gagtaaggat aaagcgcttc
    12781 gcggataatc tgattggtga cgtgcggacc ataaaagtcg ctggtatcaa tgtgattgac
    12841 gcccaatgcc agcgcctcac gcagcacggt tatagcgacg tgacgatctc ttggggggcc
    12901 aaaaacccca ggacctgcca gttgcatcgc gccataacca agacggttaa cggattttgt
    12961 accgagagta aatgtattgc tgctcatcgc accctgccct gtaataaaac ggattaagaa
    13021 aaatcagtat agaaacgaaa agagataaat aatgcgaagg cgtaaacaag atttctggat
    13081 gagtagcact attgtcctga aagacaccaa aacattagac agcaaaagat aagtcaagcg
    13141 tcgcatccgg caataaaaaa ccgcaacctg tatcagcgtt gcggtttgtg tatcacatgc
    13201 taaaaattag aacgttttct taaagttcag catcacgcct ttgtcgctga tgccgtcctc
    13261 tttccagtga tacgcatcca gattcagcga actttctttg ctactgtact tcacgccgac
    13321 gctcgccagg ctgttgatac cgccgccctt ctgaccgtca tcgacgttaa agtgctggct
    13381 gcctgcccct gcaaggcttg ccgtgcgctg gctcttgctg tagctcaggt tcggcccgcc
    13441 ttccagcgtc gcattcgcgc tccagccgcc tttccctgcg tagtccagtt tcagcccgac
    13501 gatgctgtcc accgccgttt cgctgccgct gttcatgctc aggttaaagt ctccggcatt
    13561 gcgctcctga tagccgcctt ccagcgagtg acgcagtttc actccggcgt acggggtgat
    13621 tttcagccct tcgcgcggct caaaggtttt cgccccttcg ctacgcaact ccaggtactg
    13681 ctgtttcaca tccgtatccg ccgttttgct gacgtcgccg taagcgaccg agcggctgct
    13741 gtcgagatta tgcacgtcat aacgcagcgc gttgttccaa cgcataccat tgtcgaagga
    13801 catctggtgc ttcaggccaa agaactggct gtagccgccg gttacgccat tgtcgcccgc
    13861 tttctgcgca ccgtcgccat ccagacgcgc gataccgtat tccaggctca tgctctggct
    13921 ttcgctcagg tcaacggttt tacgcagtgc cagcatgtca tactcggtgt tatttccgag
    13981 ttccgcacgc gggtcacctt tcgccaccac gttaaaggcc aggccattgc ccaccttcgg
    14041 cgcggcatcc gccagcatgc tgaagcggtt gcttaacaca cgcgcttcac ggaataccgt
    14101 ggtcgcctgg ctaccgctca cctgcttcag ggcgctattc agttcagcag tggtgcccac
    14161 gttcaggctg gtatacagct cgttattggt gtaacccgca tccagtgcct tcgccacatc
    14221 gttcaccgaa gcatcggtcg ccacatcggt gtaggcgttt ttgctcatga tgacgtcaac
    14281 gttgccgctg gcatcggtgc tgcctttggc ggtccatacc acggacgttg aggtgatggc
    14341 atcggcgtcg gtcaggttga tgccttccac cacgttgtcg aaactgaccg tggtgtctgc
    14401 cgtaccggcg gcgaaaccgg tgttgatccc gacaccattc atgctggcgt tgttgacctt
    14461 cagtttaccg gcgctgccgt caacgttggt accgacgata tagccgttga ggttgttact
    14521 gctgccatcg ctactaccgc tatcagtcgt ttcactgaaa ggtgaagtgg tgtaagtgtt
    14581 tggcatgtcc ggcaacgtgt agtcggtgta atgcacttcg gtattgttac cgctgttgcc
    14641 gttcacccct tccacattca cgctgtcgcc ctgcttaatc aaccccgaac cagtcacgcc
    14701 gtcggcaatc accaccgtac cgttgttaac gatatgacct tctgtgccca gtacgctgaa
    14761 cgcaaacgag tcgttagcaa aaatattgat cgtgccgttg ttctcaatta ccgcatcggc
    14821 ggtggcattg gcatccagtt gcataccagt caatccggta tcggtggtgc cttcggtacc
    14881 gagattgata acgccgttgt taaccagctg tgcgttttta tcgccttcca tcgccacggc
    14941 gttgttgccg ctgatgttca ttacaccatc gttgagagca acatagttgt tgtgggtgaa
    15001 tttaaccgcg ctggcgttgt ctttatcgaa gttaattacc ccgccatcct ggttccacaa
    15061 gaaggcatcg ctttctgtcg caccggcagt acttaccgcc acggtgactt tcgcatccac
    15121 cacgcccttg ttaataaact cgctggcgcc atcgtcgtag tgaatagcgc ccgcacctaa
    15181 tgcggcatcc agcgtcattt caccggtgtt atataacgtg gcgttagcat tgctgccctg
    15241 gaagcgaatg gcgtttttgc tgttatcaag gatgagtttg ccgctgttgt tgaacgttga
    15301 cttatcgtca atatccagcg tcccggcatt gttgttttca tcctgataac ccattttcca
    15361 ggtacccgcg ttattcacta ccgcagagtt atagagaatc gcgcctttac gcgcctcgcc
    15421 gcctgcaagg ttattgaata ccgcatcgcc ctgagcgtta acgcgcgccg acgcaatacc
    15481 gctgttattc accgtaccat cgtgaagttg taaagttgac agggaaccgg tgttattaat
    15541 taccgcgttg ctgtttttct caatttttac gacgtcatta agcgttccgt tgttattgaa
    15601 ggttcctcct tccagagtca ccgtgttcaa tacaccttca ttggttaacg caccgccttc
    15661 attcacgacg atggctgagc taatggttcc gctcgactcg ttaaccagcg caccattttc
    15721 gagtatcctg atcgttccat ttttaacagc acccgcactg gtgacatccc cgttgataat
    15781 cgcacctgac gataataata cggtctcacc cgcagaactt aatacagcgc catcatcata
    15841 taaatcagca atataattat ccgttggatt atattcatcg gccccctagc aaacgccaaa
    15901 ggtacaaatt gtgccatagt tgataataag cccagtgcca tcgttataga atgcctgacc
    15961 attttccgcg ttaatgttga ttacgcctgt ctggtcatta atggcggtgc cttcgtgata
    16021 aaccgccatg ccgacgagtt tattcacacc gagagaatcg ttgtaatttt catttttctc
    16081 caggttgatg gttccctggt taatgaccgt attagtattc ccatacgccg ccatccccgc
    16141 accggcgttg tagacagtaa tcacaccatt ctcgtggttg gttgccgtgg ctttagttaa
    16201 tgccccatta taggcatcgg tacctgaagc cattcccaca ccgaaatctt tcgccgtgct
    16261 atttgcaaca ttattacgca gcgatgtttc atcattttca tcaacccaca gcgtatccag
    16321 ggtgatttgc ccttcgttat ccgcggttga tttatcgatg gctagcatcc ctacactgcc
    16381 acggccatac atatcaataa tacccgtttc gccgttcaga atggatgacg agtagctggc
    16441 aagcatacct gtcagcccta atgccttgtt attgaaagag gtaccatcag acaagttcca
    16501 ctctgccgga taattcagca cactgtgctg attcatcatt ttggtggtca tggtaccatt
    16561 gttaacgcca atcccaccgt attcaacatc aaagactttt ggtgccgggt cggtaccaaa
    16621 atcgtacatc gccaggccaa tggatccgtt attgattacc gttgaatctt tattccaggc
    16681 agccgcaaaa ccgatatttt tcagttccac ttcaccgttg tttgtcgcct tcgcaccaga
    16741 atcaatacta atggtggata acgctacacg ataataataa gtgaaagaat ccacatcact
    16801 catatcaaac gttgcgtcag cgccaatctc cagctccgcg ccatggctca actgaatgag
    16861 attttcgaac cccagcggga actcgccgct gatggttgac tcaccattca ggtagaccgg
    16921 ggaatgccca tcaacgttaa taagcggtgt gtctccgata ccacgtcgag aagctgatgg
    16981 ttttgagcca tcatccatga catttttttc accgacaaaa tggacaccgc cattaagcgt
    17041 aaaggtattg tcatcgccag caataacaat acctgcaatg tgttcctgag caccgtcgta
    17101 actcgcatag gaacgcgacg tggttttact atcagcaaca acggttaact gaccatccag
    17161 cgtgacgtcg ttgttgtcac cgctgatatt taccccggtt gagggattaa agaagtaagg
    17221 ctgcgcatta tcggctttct gatctttatc aaccagaata ctgcccgcta tttcgatagt
    17281 attaccatca cccgaaacat taacacccgt cgctttttgc cctgtcacat tcagctcata
    17341 agtcgccagc gtcatgttgt tattattgcc agagacatca agaccggtgg agaaatcccc
    17401 aacgtctaac ccacccgcga gtgaaatatt agctttatcc ccagcaacgc tgacaccggt
    17461 tccggcattg gtcgcggaaa tatcaccaac gttaacaaat gtcgcattat caccgtcgat
    17521 ctgaatgcca atggcatttt cacccgctgc ggcctgacca acattgaggt cgcccatgtt
    17581 gatcacgctg acgccatcac catcaagcag catgccaata gagttatctt cgatgaccgt
    17641 aatattacct ttgttgctaa cgaacgtatc attgccgtca acgtcaatac cgtgcgcacc
    17701 accgcttacg tataagtcgc cttcctgttt gatggtggca ccgtcaccga caattttcga
    17761 gccagtggaa ttctctccgt caacgctcat ggtaccttga ttatcgaccc gcgcattatc
    17821 gccattgata aggctacccg taccaccatt ggtgatggtc gagtcgcccg cagtgtttag
    17881 cttggtatta tcgccattga tgattaccgc ggtaccgcca tcaacttccg tctcgccatt
    17941 attgttaatg gtgatattgt cgccagtgag tataccgaca accgagcctt ctccgatggc
    18001 cgtcgttttg ccgtcgttgt tgagggagat gttgtcacca tcaaccgtca tgacagtacc
    18061 gccattcagc gccgtgttgc cgccctgatc ggtgatgatc gcgttaccaa actcaatcgc
    18121 attcccggtt gtgccgtcaa caacgacgcc ggcctcttta gtgatgtgga tgagtccatc
    18181 atcgtcgtaa ctccaggtga tgccacctga ggtatttacg ccatcaaaga caacggtgtt
    18241 gctgtcgtca tggactgtcc aggacttaac tatcgtctcc ttgccctcag gcgccgttaa
    18301 ggtgtagctc ccatcggcat tttggctgta ggtaaaggta gtttcgcgaa ttttgacagt
    18361 ttttgcatcc tgatcccagg ttacgccatt tttatagacg acaggattgt gtttaaccgg
    18421 ggtatcgcca ccatcgtctg gcggaactgg cgtatcaccg ccgtcatctg gcggaaccgg
    18481 cgtatcaccg ccgtcatcag gcggaactgg cgtatcaccg ccgtcgtctg gcggaacagg
    18541 cgtatcaccg ccatcatccg gaactggcgt atcaccgccg tcgtctggcg gaacaggcgt
    18601 atcaccgcca tcatccggaa ctggggagtt attatggtgg tgattgtcat cgtcacttaa
    18661 ggtatatata gcaattcccg tagctactgc agcaagacct gcagctgccc atggtaaaac
    18721 tgcactatct ttattttctc tgcactccgc ggataaattt tctagctgct ctttgctcag
    18781 ggaatgaata tttgccggac atgttatctt actggactta cgtgtttttt cggttgagtc
    18841 gggtattgaa gtatccgcag cccatgtctg gctacttaac gccatggcaa cacaaacaga
    18901 caataatttt ttcctttgca tataaaattc cttagatcta gaccgtgcgt aacaagccat
    18961 gcagaatttg aagataaatc gtagtaaata ttcatagtga aataagcagt aaagaaatgc
    19021 ttatataatt cacaaaatca atattaaaat caggcaagta aaagcataca aaaaaaacag
    19081 ttgatggaat tatattccac aaaaaactca cttcaatcta aaaattaacg acaaaaaaca
    19141 aaaacacact aataattcat tgacgctgca tatatatttt gtgattatat atatattggt
    19201 aatgatttgt tatttaaggg aaggatatat aaggtttttg tagaaatacc cacggtaatt
    19261 aagcatgcta acgtcagttt aaatactgta aaagatcatt acattaatag atacattttg
    19321 tagccaacta taatttattt gcgcaaccat ttaccattaa ctccctgaac atattgcccc
    19381 gacttcgcgc gagcaacaag cttttgtcca gccagtttgg ctatctcttt agtggataca
    19441 ttattttgct ttgccagttg ttggtaacta tggttacgag cttcattaat atcttttact
    19501 aatgcctgtg tttcggcatc cgtttgtagc gccaccaggt aaccgttaag cgtttcacct
    19561 acccgcccct gacttctggc ttcgttcagg gttaaagcca ttgcactgtc gctcatcagg
    19621 ccaatggccc atacagctat gagtaatgtt cttttcatca tcacctcaaa atagatcgct
    19681 acgggttttg agcaggtctt caacgtcctt atccgcttta atgatgattt catgctcaat
    19741 tttaacgttc atattaatgg tgattggctc tttcggcgca gccacttcaa tgcgaggcgt
    19801 gcaaccaacc aacataaaag aggacgcaaa cgcggctatc actattttca ttgtttttcc
    19861 tcacactctt ttcctgatgg gcagccagtt tccggtagct gggcattctg ctccagccat
    19921 acctggagat tatcgccaaa gcgtaaactg cgccataacg taaaaatatt ctcttcatgg
    19981 tgataattaa ggtttaccgt accgctttta ccatcgacgc gactggtgcc cgaaatgttg
    20041 gcctgcatgg ttaacacacc gagattatct aaattaatcc ttgttgatga gcgactgatt
    20101 tccatataac gcaaccagtt gatggcagtt ccagcggtca tattgtcttt ggccaccgca
    20161 tctgcagtgt ctttatccag acgcagcgtc atcggccccg cgtttgccaa ccagccatct
    20221 ttgacaatcc atttttcatt gttcagccat agtggcaacg ccccactaaa tgccccagac
    20281 atagcaaact gtttcggatt aaccgcgcta accagttcgc tggatgagag attattcagc
    20341 cgcaacagcg ccgggtcatg ttgcggcata cgtaattgtt tcatcagcac gttaccgcct
    20401 aacacatcga cgctaacatc agtcaacagc aatggttctt cttcagtcca cggataacgc
    20461 ccttgcaaat cagccgtaat atttttcgct gtcaccagat taatcacttc ggcaattcgc
    20521 aacgtaacgg ggccgcgagt ccccagatgc caggctccat cggcaaaacg gaaaggcagg
    20581 acaaaatcga caccgttaac ctgattatct ggcatccagg cactaccgcc tttcaacacg
    20641 ccgtgccctc ccgcgcggaa tccttgttca ggcgcagcag aaaatgcaac ctgagcatac
    20701 agttcaccat cgcgtaagtt catcttccag tcgggtggca ccagcggctg gaatacagtc
    20761 agtgattgtt taggccacca ggcgttaccg cgcagacgaa taccgtccca gcgaccattt
    20821 acccgaaccg ggccaatctc accagcatgt aaatcgcctt taaagaggaa atacgtcgga
    20881 tcgcgcccat cgacgctaaa ttttaaggtt gatggcggta acacactgcc gccagtgaac
    20941 agcgtttgcc cggcgtccag tgacagcgcg ccgctgaagg agggatgctg tgcgtcacgt
    21001 acccagacga cgggcttgtc gagaattaat cgcggctttt ctaccgtcat cgtaccgtat
    21061 tgtaactggt cgaaaccggt ggagagatcg gtcagcgtaa tcgtgctgtc atgccactca
    21121 ccttttcctg cgacatccca ggtggcattc atcggtgtaa aactcccttt tccccagtag
    21181 cgccactgcc agcggccagc gtcagggaga aaatcattcg ccagcccatc catatgcagc
    21241 acgaaatcgc ccagttcatt ttcatgcgcc tgcaaaatgg cctgcaaacg tccgtcaaca
    21301 ccacgttggg tgacttttac acccgctaaa ggccagcgga tttcatcgat gtccagcgaa
    21361 tcgatgactc ttccctttga acgaagtaac gcgcctggct caaaggtcag cgttgggtca
    21421 gacagacttc cacttagctg cgcaggtaaa cgggcatata aaatgaggtc cgcctgtttc
    21481 gcttcaccgg tcagctgcag aggcagctgg ctgttatcca tgcttaattt tcctgggcca
    21541 aaattaagca ccgcgttgcc cttacccgct tgcccctggg tcagcacact cagtcgtccg
    21601 ctgaccagag cgttctcaag ccctgcctgc cagttgtcga ctttgacacc cagtcggcca
    21661 ctcaaaggaa aacctgcata cggccagctc cagcgaccat cgcttacggt caattgttga
    21721 cgagtaattt gccacggcaa atcgagcaac ggatcgccgt tatcccgtgc cagcacgatc
    21781 aattgcccgc tattttcctg ccagtccagc tcggcatcca ccagtgacgg ttcctgcggc
    21841 aagtttaacg tcgcagtagc atgcccactt acaggaagtc catccggcat gagcggcata
    21901 gtaaattccc ccaccagttt aaccgacggc tgattttcaa acgcgacgac atccagttcg
    21961 ctgactgtaa gttgttgccc tttcagctgg ccttgaaatt taactttttc gccctgataa
    22021 cgcagttgct ggatatcaga ggttaatgcg agagagagtt ttccctgcca ttcctgccag
    22081 ggagaaaaaa tcagtttatc aatattgatc caggtgttag gcagcatgga ctgccattgc
    22141 gcgagggttt ttggagcagc tggcgattgc tccgtctgcg gcaatttcgc cagacaagca
    22201 gaatcaagtt ctaccgtgcc gacgttcaat aaccagcggc tgggatgtga aaggctggcg
    22261 ttggtgatat gcgcaagctg acaatctccc accagataac ggagatcggg aatgattaaa
    22321 cctttacgcg taatgcgtgg gctttcatct aatgcaatac gtgtcccgag cggtagccag
    22381 atgcccgcca gcgtgggaac ccacagcccg agcgtcatca gcagcgtcaa cggcacaaga
    22441 ataatcagta ataacagcgc gagaacggct ttatatttac ccagcatggg tagttaatat
    22501 cctgatttag cgaaaaatta agcattcaat acgggtattg tggcatgttt aaccgttcag
    22561 ttgaaggttg cgcctacact aagcatagtt gttgatgaat ttttcaatat cgccatagct
    22621 ttcaattaaa tttgaaattt tgtaaaatat ttttagtaac ttaaatgtga ttcaacatca
    22681 ctggagaaag tcttatgaaa ctcgccgttt atagcacaaa acagtacgac aagaagtacc
    22741 tgcaacaggt gaacgagtcc tttggctttg agctggaatt ttttgacttt ctgctgacgg
    22801 aaaaaaccgc taaaactgcc aatggctgcg aagcggtatg tattttcgta aacgatgacg
    22861 gcagccgccc ggtgctggaa gagctgaaaa agcacggcgt taaatatatc gccctgcgct
    22921 gtgccggttt caataacgtc gaccttgacg cggcaaaaga actggggctg aaagtagtcc
    22981 gtgttccagc ctatgatcca gaggccgttg ctgaacacgc catcggtatg atgatgacgc
    23041 tgaaccgccg tattcaccgc gcgtatcagc gtacccgtga cgctaacttc tctctggaag
    23101 gtctgaccgg ctttactatg tatggcaaaa cggcaggcgt tatcggtacc ggtaaaatcg
    23161 gtgtggcgat gctgcgcatt ctgaaaggtt ttggtatgcg tctgctggcg ttcgatccgt
    23221 atccaagtgc agcggcgctg gaactcggtg tggagtatgt cgatctgcca accctgttct
    23281 ctgaatcaga cgttatctct ctgcactgcc cgctgacacc ggaaaactac catctgttga
    23341 acgaagccgc cttcgatcag atgaaaaatg gcgtgatgat cgtcaatacc agtcgcggtg
    23401 cattgattga ttctcaggca gcaattgaag cgctgaaaaa tcagaaaatt ggttcgttgg
    23461 gtatggacgt gtatgagaac gaacgcgatc tgttctttga agataaatcc aacgacgtga
    23521 tccaggatga cgtattccgt cgcctgtctg cctgccacaa cgtgctgttt accgggcacc
    23581 aggcattcct gacagcagaa gctctgacca gtatttctca gactacgctg caaaacttaa
    23641 gcaatctgga aaaaggtgaa acctgcccga acgaactggt ttaatcttgc cgctcccctg
    23701 caatccaggg gagctgattc agataatccc caatgacctt tcattctcta ttcttaaaat
    23761 agtcctgagt cagaaactgt aattgagaac cacaatgaag aaagtagccg cgtttgttgc
    23821 gctaagcctg ctgatggcgg gatgtgtaag taatgacaaa attgctgtta cgccagaaca
    23881 gctacagcat catcgctttg tgctggaaag cgtaaacggt aagcccgtga cctgcgataa
    23941 aaatccgcca gaaatcagct ttggtgaaaa aatgatgatt tccggcagca tgtgtaaccg
    24001 ctttagcggt gaaggcaaac tgtctaatgg tgaactgaca gccaaagggc tggcaatgac
    24061 ccgtatgatg tgcgctaacc cgcagcttaa tgaactcgat aacaccatta gcgaaatgct
    24121 gaaagaaggt gcacaagtgg atctgaccgc gaaccagtta acgctggcga ccgcgaaaca
    24181 gacattaact tataagctgg cggatttaat gaattaatag ctgccacagc tcccggcggc
    24241 aagtgactgt tcgctacagc gtttgccgtt gggtaatgca cacatcccaa tcgccgtacc
    24301 atccagttga cgggcaacag aaagcgaacc gccgatcatt gcacaatttg cttctccact
    24361 actggacatc gacgctttta aacctggcgc tacgtgcgcc gcagtcgcct gctgaacagg
    24421 ctcactacta cacgccgaca acaataaagc ggcacaccct acccaaaacg ctgctcgcat
    24481 ctcttcttcc tctgatcttc aagccaaacg acaccgccat aaataatagg cagcacagag
    24541 ggcgacgtcg agagctgtcc tgcgcgttgc cccgcatttt tactttttta tggctatttt
    24601 tttgccctct gtttgatcaa aacattcatt acgctgatgt gggggacaca aaagcgaaaa
    24661 tgcagaagaa agccatttgc taaaattgaa agattactac tgggcgcgca gcaatttcgt
    24721 gcgcccctca tttgcgcaat gtaagggtgt catatgatta ctattgacgg taatggcgcg
    24781 gttgcttcgg tggcgtttcg caccagtgaa gttatcgcca tctaccctat tacccccagt
    24841 tccacgatgg cagaacaggc tgatgcctgg gccggaaacg gcttgaagaa cgtttgggga
    24901 gacacaccac gcgtggttga aatgcagtcg gaagcgggtg ctatcgctac cgtgcatggc
    24961 gctttgcaga cgggtgccct ttcaacatcg tttacgtcat cgcagggttt gctgctgatg
    25021 atcccgacgc tgtacaaact ggcaggcgaa ctcacaccgt ttgtcctgca tgtagcggca
    25081 cgtaccgttg ccacacatgc actctctatt tttggcgatc attccgacgt catggcggtg
    25141 cgccagacgg gttgcgcgat gttgtgtgcg gcaaacgtcc aggaagcgca agactttgct
    25201 ctcatttcgc acatcgcgac gctgaaaagc cgcgtgccat ttattcattt ctttgatggt
    25261 ttccgtacgt cccacgaaat caataaaatt gtcccgctgg ccgatgacac tattcttgat
    25321 ctcatgccgc aggccgaaat tgatgctcat cgcgcccggg cgctcaaccc ggaacatccc
    25381 gtgatccgcg gtacgtcagc caatcctgac acttatttcc agtctcgcga agccaccaac
    25441 ccatggtaca acgcggtcta tgaccatgtt gaacaggcga tgaatgattt ctctgccgcg
    25501 acaggtcgtc agtatcagcc gtttgaatat tacgggcatc cgcaagcgga acgggtgatt
    25561 atcctgatgg gctctgccat tggcacctgt gaagaagtgg ttgatgaatt gctaacccgt
    25621 ggcgaaaaag ttggcgtgct gaaagttcgc ctgtaccgtc ccttctccgc caaacacttg
    25681 ctgcaagctc tgccgggatc cgtacgcagc gtggcggtac tggacagaac caaagaaccc
    25741 ggtgcccagg cagaaccgct ctatctggat gtaatgaccg cactggcaga agcctttaat
    25801 aatggcgagc gcgaaactct gccccgtgtc attggtgggc gctatggtct ttcatccaaa
    25861 gaatttggcc cggactgtgt actggcggta tttgccgagc tcaacgcggc taaaccgaaa
    25921 gcgcgcttta cggttggtat ttacgatgat gtgaccaatc tgtcactgcc gttgccggaa
    25981 aacaccctgc caaactcggc gaaactggaa gccttgtttt atggccttgg tagtgatggc
    26041 agcgtttccg cgaccaaaaa caatatcaag attatcggta attccacgcc gtggtacgca
    26101 cagggctatt ttgtttacga ctccaaaaag gcgggcggcc tgacggtttc tcaccttcgc
    26161 gtgagcgaac agccgattcg ttccgcttat ctcatttccc aggctgattt tgttggctgc
    26221 caccagttgc agtttatcga taaatatcag atggctgagc gtttaaaacc tggcggcatt
    26281 ttcctgctca acacgccgta cagcgcagat gaagtgtggt cgcgcttgcc gcaggaagtt
    26341 caggccgtgc taaatcagaa aaaagcgcgc ttctatgtga ttaacgcggc gaaaatcgcc
    26401 cgcgaatgtg gcctggcggc ccgtattaat accgtcatgc agatggcttt tttccatctg
    26461 acacaaattc tgcctggcga tagcgccctc gcagaattgc agggtgcgat tgccaaaagt
    26521 tacagtagca aaggtcagga tctggtggaa cgcaactggc aggctctggc gctggcgcgt
    26581 gaatccgtag aagaagttcc gttgcaaccg gtaaatccgc acagcgccaa tcgaccgcca
    26641 gtggtttccg atgccgcccc tgctttcgtg aaaaccgtaa ccgctgcgat gctcgccggg
    26701 cttggtgatg ccctccccgt ttcggcgctg ccgccagacg gcacctggcc gatgggcacc
    26761 actcgctggg aaaaacgcaa tatcgccgaa gagatcccca tctggaaaga ggaactctgt
    26821 acccaatgta accactgcgt cgccgcttgc ccacactcag ctattcgcgc aaaagtggta
    26881 ccgcctgaag cgatggaaaa cgcccctgcc agcctgcatt cgctggatgt gaaatcgcgt
    26941 gatatgcgcg ggcagaaata tgtcttgcag gtggctccgg aagattgcac cggttgtaac
    27001 ctgtgcgtcg aagtttgccc ggcgaaagac cgtcagaatc cagagattaa agccatcaat
    27061 atgatgtcgc gcctggaaca tgtcgaagaa gagaaaatca attacgattt cttcctcaac
    27121 ctgccagaaa tcgaccgtag caaactggaa cgtattgata ttcgcacatc gcagctgatt
    27181 acaccgctgt ttgaatattc cggtgcttgc tccggttgtg gcgagacgcc gtatattaaa
    27241 ttgctgactc agctctatgg tgaccggatg ttgatcgcta acgccaccgg ctgttcttca
    27301 atttatggcg gtaacctgcc ctctacaccg tataccaccg atgccaacgg tcgtgggccg
    27361 gcatgggcga actctctatt tgaagataat gccgaatttg gccttggttt ccgcctgacg
    27421 gtcgatcaac accgtgtccg cgtgctgcgt ctgctggatc aatttgccga taaaatcccg
    27481 gcggaattac tgacggcgtt gaaatcagac gccacgccag aggttcgtcg tgaacaggtt
    27541 gcagctttac gccagcaact caacgatgtt gccgaagcac atgaactgct gcgtgacgca
    27601 gatgcactgg tggaaaaatc aatctggctg attggtggcg acggctgggc ttacgatatc
    27661 ggctttggcg gtctggatca tgtattgagt ttgacggaaa acgtcaacat tctggtgctg
    27721 gatacgcaat gctattccaa caccggtggt caggcgtcga aagcgacacc gctgggtgca
    27781 gtaactaaat ttggcgagca cggcaaacgt aaagcgcgta aagatcttgg cgtcagtatg
    27841 atgatgtacg gtcatgttta tgtggctcag atttctctcg gcgcgcagct gaaccagacg
    27901 gtgaaagcga ttcaggaagc ggaagcgtat ccgggaccat cgctgatcat tgcttatagc
    27961 ccgtgtgaag agcatggtta cgatctggca ctcagccatg accagatgcg ccaactcaca
    28021 gctaccggct tctggccgct atatcgcttc gatccgcgtc gtgccgatga aggcaaactg
    28081 ccgctggcgc tggattcacg cccgccgtca gaagcactgg aagaaacgtt acttcacgag
    28141 caacgtttcc gtcgtctgaa ttcgcagcag ccagaagtgg cagaacagtt atggaaagat
    28201 gctgcagctg atttgcaaaa acgctatgac ttcctggcac aaatggccgg aaaagcggaa
    28261 aaaagcaaca ccgattaaat gctctggata aggattatcc aattctaaaa aaaagcccgg
    28321 acgactgttc gggcttgtct ttttatatgt tggaaaatca gtggcaatgc aatggcccaa
    28381 cagaaaataa aatctgtgac aataaaggca tataacccgc gcagaataac gtatagaaaa
    28441 ttaaaagtat aatttttatt ttgtatgtat tatcttcgat acattcacca gacttattat
    28501 catttcagag gaattatttg attaaggttt tacttaaggc gtaacaaatg atatttatcg
    28561 gctaactgaa cttctccttt aggatgtttt cactccccga aatggggata aaaggcaaat
    28621 aaaataacta aaggatttat tcaatgaaaa gcaaagtact ggcactttta attcctgccc
    28681 tgctcggcgc aggtgctgca catgcagccg aagtttataa taaagacggc aacaaattag
    28741 atctgtatgg caaagttgat ggcctgcatt atttttctga taattcagcg aaagatggcg
    28801 accagagcta tgcgcgtctg ggttttaaag gcgaaaccca aattaacgat caacttactg
    28861 gctacggtca atgggaatac aatattcagg caaacaacac tgaatcttca aaaaaccagt
    28921 catggacccg tctggcattt gccggactga aattttcaga ttacggttct ttcgattacg
    28981 gacgtaatta tgggctggac cgatatgctg cctgaatttg gcggtgactc ttataccaat
    29041 gcagacaact ttatgactgg tcgtgccaat ggcgtcgcga cttatcgtaa tactgatttc
    29101 ttcggtctgg taaatggtct gaacttcgcg gtgcagtatc aaggtaacaa cgaaggtgcc
    29161 agtaatggtc aggaaggcac caacaacgga cgtgatgttc gccatgaaaa cggtgacggc
    29221 tggggtcttt ccacaacata tgatttaggc atgggcttta gcgctggtgc ggcatacacc
    29281 tcttctgacc gcaccaatga ccaggttaac catactgcgg cgggtggtga taaagcagac
    29341 gcgtggactg ctgggctaaa atacgatgct aacaatattt acctggcaac catgtattca
    29401 gaaacgcgta atatgacccc gtttggcgac agcgattatg ctgttgcaaa caaaacccag
    29461 aactttgaag tcactgcaca gtaccagttt gattttggtc tgcgtccggc agtctctttc
    29521 ctgatgtcta aaggccgtga cctgcacgct gccggtggtg cagacaaccc ggcaggtgtt
    29581 gatgataaag atctggttaa atacgccgat gttggcgcga cttactattt caataaaaac
    29641 atgtccactt atgttgacta taaaatcaac ctgttggatg aagatgacag tttctacgct
    29701 gccaacggca tctctaccga tgatattgtc gctttaggtc tggtttatca gttctaaatc
    29761 ctcctgcccg ctgttatggc gggctttttc tgcttattct tcctcctttg atctaaatta
    29821 aaaatgtgaa ctccgtcatt acacaaaaag tgtcatctgg cgttacactt tatgcggata
    29881 ctaaaacagg aggttttatg aacagaacga ttcttgtccc tatcgatatt tccgattcag
    29941 aattaactca acgcgtgatt agccacgttg aggcagaggc aaagattgat gatgcagagg
    30001 ttcatttcct gacggtaata ccttcactgc cctactatgc ctctctgggt ttagcgtatt
    30061 ccgcagaatt accggcaatg gatgacctga aagcggaagc caaatcgcaa ctggaagaga
    30121 tcattaaaaa atttaaactg ccaaccgaca gagtgcatgt ccatgttgag gaaggctcgc
    30181 ccaaagaccg cattctggaa ttggcgaaga agatccccgc tcatatgatc atcattgctt
    30241 cccatcgacc ggatatcacc acttatctgc tcggttccaa cgccgcagct gtagtgcgtc
    30301 acgcagagtg ctccgtgctg gttgtgcgct gacactaacg cccgcacatt gctgcgggct
    30361 ttttgattca tttcgcaaat gtgctgacat tttcccctct aatccgtacc atacacgcca
    30421 cagtttttat atcagatttc ttatgctggg cgttccggca tgacgcatac tcttctgatg
    30481 ccatataacg aattgagtcg cttttaaatg tcgcaaaatc aagaaattag caagaaagaa
    30541 caatacaacc tgaacaaatt acaaaaacgc ctgcgtcgta acgtgggcga agccattgct
    30601 gacttcaata tgattgaaga aggcgatcgc atcatggttt gcctctccgg gggtaaagac
    30661 agctatacca tgctggagat tctgcgcaat ttgcagcaaa gcgcgccaat caatttttcg
    30721 ctggtggctg ttaacctcga tcaaaagcaa ccgggcttcc cggaacacgt tctgcccgag
    30781 tatcttgaaa agctgggcgt tgagtacaag attgttgaag agaacactta cggtatcgtg
    30841 aaagagaaga ttccagaggg caaaaccact tgctcactgt gttctcggct acgtcgcggt
    30901 atcctttatc gcaccgcaac ggaactgggg gcgacgaaga tcgcgttggg ccaccatcgt
    30961 gacgatatcc tgcaaacgtt gttcttaaat atgttctacg gcggcaagat gaaaggtatg
    31021 cctccgaaac tgatgagcga tgatggcaaa catatcgtta ttcgtccgct ggcctactgc
    31081 cgcgagaaag atattcagcg atttgccgat gcaaaagcgt tcccgattat tccgtgcaac
    31141 ctgtgcggtt cacagcctaa cctgcaacgt caggtgattg ctgacatgtt gcgtgactgg
    31201 gataaacgtt atccagggcg tatcgagacg atgttcagcg cgatgcagaa tgtggtgccg
    31261 tcgcatctgt gcgataccaa cctgttcgat ttcaaaggca tcacccacgg ttctgaagtg
    31321 gttaacgggg gtgatctggc gtttgatcgc gaagagatcc cactacaacc ggcgggctgg
    31381 cagccagaag aagatgaaaa tcagttggat gagttacggc tgaatgtggt tgaagtgaaa
    31441 taactcggat agcgcccgat gcgcaaacgt atcgggctac tctaatggag gctgaataag
    31501 acgcggtcag cgtcgcatcc gacaatcccg acgaagatgt ttacttttgc atcaggcaat
    31561 tcaacatttt attattttaa taaccgcacc cggcacgttt ttcctttaat cttcccgccc
    31621 tgtaactgtt tccatgcttt atgagcaaca gcctgacgga ccgcgacata gacatgcgcc
    31681 ggatgcacgg cgattttgcc aatatctgcg ccatcaagcc cgatatctcc tgtcagtgca
    31741 cctaatacat cacccgggcg cattttggct tttttcccgc catcgataca caacgttgcc
    31801 atttctgctt ccagcgtcac aatggaacta ttagctggtg gcgtttgcca gttaagtttt
    31861 atctgcaaca tgtcagaaat gatattggcc cgctgtgctt cttccggagc acagaaactg
    31921 atcgccagac cgctatttcc tgcacgagct gtacgaccga tgcgatgtac atgaacttca
    31981 gggtcccacg ccagctcaaa gttcaccacc agctcaagcg atttaatatc cagaccacgc
    32041 gcagcaacat cagtcgcgac cagtacacgg gcgctaccgt tagcaaaacg taccagggtc
    32101 tgatcacgat cgcgttgctc cagatcgccg tgtaatgaca atgcactttg ccctacttca
    32161 ttcagcgcgt cgcagacagc ctggcaatct tttttggtat tgcaaaacac cacgcaagag
    32221 gatggctgat gcaagcttaa taaccgttgc aacagaggaa ttttgccttt gctggatgtc
    32281 tcataaaatt gttgttcaat gggtggcaaa gcatctgttg agtcaatttc aatcgccaaa
    32341 ggatcgcgtt gcactcgtcc gctgattgcg gcgatggctt ccggccaggt tgccgaaaac
    32401 agaagcgtct gtcgagatgc aggcgcaaaa cggatgacat catcaatggc atcgctaaat
    32461 cccatatcca gcatgcggtc ggcctcatcc atcaccaaag tattcagcgc atccagtgat
    32521 accgtgcctt tttgcaggtg atccagcaaa cggcccggcg ttgccacgat aatatgcggc
    32581 gcatgttgca acgaatcacg ctgcataccg aacggttgac caccgcacaa cgtcaaaatt
    32641 ttggtatttg gcagaaaacg cgccagccga cgcaattcac ctgccacctg atccgccagt
    32701 tcacgcgtag gacacagcac taaagcctgg gtttgaaata gcgacgcatc aatttgctgt
    32761 aacaagccga ggccaaaagc cgccgttttg ccgctgccag ttttcgcctg cacgcgaaca
    32821 tcttttccgg caaggatcgc cggaagcgcg gcggcctgca ccggcgtcat ggttaaataa
    32881 cccaactcat taaggttcgt gagttgggcg ggaggcaaaa cattcagggt agaaaaagcg
    32941 gtcacaatct attctcgtgg tcatcgacgc aaagtcatca ggcgcgtatc ctcgcagatc
    33001 tacgctcacg atgcgacaat ttaatcggtt cttcatcggg tggtgggtca ggcatgggtt
    33061 gcgggcgagg gatcggatcg ggcactggaa caggatcgcc aggaatcggt tcagggacag
    33121 gaatttgcaa ataaataagt gtcgtcatat ttccctccgg tcattgggtg gactcttaaa
    33181 gggtagacgc tgataaataa caggcaaaaa aaagccgact catcaaagtc ggcgtcgtac
    33241 gaatcaattg tgctatgcag taattcaaaa aaggaagtaa gacaatatgg agcgcaacgc
    33301 ccatcgcttg acgttgcatt cacctgcaag agagatattg ccctgaatgg gtagagagtt
    33361 tattgacttc gctcaaactt tgcggcgttt ttgtatacag acagccggaa aaattgcttt
    33421 tgttacaacc atttactacg atgcaaccat aaagcaacac caccaataag aacaactaac
    33481 agaatacaaa aaattgaaaa tccgaattgc cacccgccgc cagggatccc accaaggttg
    33541 acgccaaata accctgtcag aaaggtactg ggtaaaaaga ccattgccat caatgacatt
    33601 gtataggtac gacgagctaa attttcctgc atcacctgag cgatttcatc cgccatcaca
    33661 ccagtccgtg ctatacaggc gtcgatttcg tcaaggccgc gcccaaggcg atcggcaata
    33721 tcctgcatcc gacggcgttg gtcatcgctc atccacggca aacgttcact ggcaagacga
    33781 gcataaacat cacgttgcgg tgccatatag cgacgcatca caattaattg tttgcgcagc
    33841 agagccagga atccacgcgg cggaatttgc tgatcaagga gattatcttc aaggtcgata
    33901 attttatcgt gcagctgctc gataaattcg ctggaatgat cggtcaacgc atcgcacaca
    33961 tccaccagcc atcccccgca atcggtcgga cccgtgccct cttccagatc actcaccaca
    34021 tcgtccagcg ccagcacttt gcgttgtcgg gtcgaaacaa ttaaccgccc gtccatatat
    34081 acacgcatgg cgaccagttg atcggggcgt tcatcggtgc tgccgtttat acagcgcaat
    34141 gtaatcagcg tgccttcacc gagacggctg actcggggcc gtgtgctctc gcccgccagc
    34201 gcatcacgta cgttattggg aagcagcggt gttgtcgcca gccattgggc gctatcatgg
    34261 tgtacataat taaggtggag ccagcaggga tgcgcttcat caatcacatc tgtattttcc
    34321 agcggtttaa cgccgcctct accatccagc atccaggcaa atactgcatc cgggacatta
    34381 acgtccgatc ccttaatcgc ttccacagtg cctccatcat caacgcatta ttttgtagtc
    34441 tagccttctg gccctgttac gcaacatctc atcaccccat taccctgaaa tgattaataa
    34501 aattctgtct aaattgaata caaaaagcaa aatgcttttc cgtatacaaa ccgtgtgaag
    34561 tgttaaatag cgtctatcat tatcagaatt atctgatcat atgacgtggc ttttttgcga
    34621 tcggatagca acaaaaattg ataaaaataa tgggatctca atgattacgc acaacttcaa
    34681 tactctggac ttactcacca gtcctgtctg gatcgtttcg ccctttgagg aacagttaat
    34741 ttatgccaac agcgcggcgc gactgttgat gcaagacctc acgtttagtc agctacgaac
    34801 cggtccctat tccgtctcct cacaaaaaga actgccgaaa tacctctccg atctgcaaaa
    34861 ccaacacgat attatcgaaa tcctcactgt tcagcgtaaa gaagaggaaa cagcattaag
    34921 ctgtcggctt gttttgcgag agctgacaga aacagaaccg gtgattattt tcgaaggtat
    34981 cgaagcgccg gcaacgctgg gtttaaaagc cagtcgctcg gcaaattatc agcgcaaaaa
    35041 acaaggtttt tatgcgcgct tttttctgac taactctgca ccaatgttgt tgattgaccc
    35101 gtcacgagat ggacaaatcg tcgatgctaa cctcgccgcg ctcaatttct atggttataa
    35161 ccatgaaacg atgtgccaga aacatacctg ggaaataaat atgctcgggc gtcgcgtcat
    35221 gcctatcatg catgaaatct cgcatttacc cggtggtcat aaacctctga attttattca
    35281 taagctggcg gatggttcga ctcgtcatgt gcagacctat gccggaccga ttgaaattta
    35341 tggcgacaag ctcatgttat gtattgtgca tgatattact gagcaaaaac ggctggagga
    35401 gcagctggaa catgctgctc accatgacgc gatgaccgga ttactgaatc ggcgacagtt
    35461 ttatcacatt acggaaccag gccaaatgca gcacctcgcc atcgctcagg attacagctt
    35521 attgctcatc gacacagatc gttttaaaca cattaacgat ctctatgggc attctaaagg
    35581 tgatgaggtg ttatgcgccc tcgcccgcac tctcgaaagt tgcgctcgca aaggcgattt
    35641 ggtgtttcgt tggggaggcg aagagtttgt cttattacta ccaagaaccc cactggatac
    35701 cgcgctttcg ctggctgaaa ctatccgcgt aagcgtggca aaagtgagta tttcgggctt
    35761 accacgcttt accgtcagca ttggtgtggc gcatcacgaa ggaaatgaaa gcatcgatga
    35821 actgtttaaa cgcgttgatg atgctttgta tcgggcgaaa aatgatggac gcaaccgcgt
    35881 gctggcggca taagccacgg atgcgtctcg agatcaacga ctgcgcttag cgtggcgctc
    35941 ccagttttct tgctttgcct gtgccgtttt acgtagtgcg acgtaacacg ccccgctgcc
    36001 gccatgatgc ggtagcgcgg tacaatatgc ctggacatca tcaaattcgg tcaaccagcg
    36061 cgccacatag ctgcggacaa tattggcatg cgatttatca tcccgccctt taccatgaat
    36121 aatcagcacg ttacgcaaac catccgccat ggcttgttga atgaaactga acatcatttt
    36181 gcggcactct tccaccggct ggcgcaaaag attcaggctc gcctgttgcg gatatttacc
    36241 actgcgcagc ttatccagca ccccatgttg caacccttcc cgccgaaact ccagcggctg
    36301 acttagcggg atgatgtcga gaaatccggt ggtgaggaaa ttatcaagtt gcagcgtgtc
    36361 gatacgctgc ggcgcacgtt ggttacgcgt tggatgccag tggacatcgg tagcacgttt
    36421 cagcggctgg acatcttcca tggcgtcaag aaacagcgat ttgtcgtcaa ggttcatgtt
    36481 acatcctccc gcaattaaga gcgcgatatg ataaccagac cgggtcggtc caacaacgta
    36541 ttacccaaaa ttccagtaat aagttccaaa tattgccgat attttaagca aaatgcttat
    36601 gcatgattat tcattcacga tattaataat gtaacttata ttttcgtgaa atctgtcact
    36661 gaagaaaatt ggcaactaaa ggttaaaacc gttataacac agtcaccggc gcagaggaga
    36721 caatgccgga tttaagacgc ggatgcactg ctgtgtgtac tgtagagtct ggcggatgtc
    36781 gacagactct atttttttat gcagttttaa ctttgcagat agccgcattc tcgccggaat
    36841 tgcgtgatta atgacgcggt cagtggtgtc tggcgactat cacgccgctg aatcaaataa
    36901 taggccgctt tcggtaaaat ttcgctaacc ggcaacatca ccagcccctg tccgtgcaag
    36961 ggatcgcagc ccatttcttc aggcagtatg ctgagaaaat cgctttttgc caccagactg
    37021 atacaggctg agaacgtctc gcagactaca ccgacctgtg gcgtttgcgc ctgatcgtca
    37081 agcaattcac tcaactgttt gtagtagctg ccgtgtggcg tcggcattgt ccagctgtaa
    37141 tccagtaact gtttgatcga acgggcacca atggcggggt gtcccgggcg gcaaaagatc
    37201 gcgaattgct tttccagtaa tttctcaaaa gtaaattcgt ggtcgtacgg tccctgataa
    37261 taggtattga tggtgaaatc caattctccc tgacgcaatt cattaatcat cgacaccagt
    37321 tgcccttcca taatgcgtac ttttacctgc ggatgctgct gatgaaaacg agatattaca
    37381 gctggcatca gactgcggga aatactggcc cccatgccga tattaatctg ccctgccagt
    37441 tgcccttgtc gttggcgaat atcctcttgg gctgcgcgca gttcttcaag aataagactg
    37501 gcgtgctgat aaaaactttc accggcatca gttaacgtca cgcctttact acggcgaaaa
    37561 aagagttgcg ccgctaaccc ttcttccagc tcctgaatag atttactcag tgccggttgc
    37621 gacatattca acattcggct cgctccgcga atgctgccct gacgagccac ttcaacaaaa
    37681 gcccgaattt gatgaatttt tacctgaaaa gccatgacgc caccgataac cgttatttat
    37741 cagaccaaag aaactggcat ctactttaat gcagatgatt gtgtcagggt aatttatgaa
    37801 cggttaaaac tgtgaaaaat cagttagtga taagtaaaaa ctatcgctac gtgaaccggg
    37861 tcacactttt tactgatgac gggaaaggtt atggagtctt tgaatcaatt tgttaattcg
    37921 cttgccccaa aattatcgca ctggcgacgt gattttcatc actatgcaga gtctggctgg
    37981 gtggaattcc gcactgccac ccttgttgcg gaagaattgc agcagctcgg ctactcactg
    38041 gcgctgggcc gcgaagtagt taatgaaaat agccggatgg gactacctga tgaactcact
    38101 ctacaacgcg aattcgagcg cgctcgtcaa cagggtgcgc tagcacaatg gattgcggct
    38161 tttgaaggtg gtttcacggg tatcgtcgcc accctggata ccggtcgccc cggtccggtg
    38221 atggctttcc gtgtcgatat ggacgcgctg gatctcagtg aagagcagga tgtcagccat
    38281 cgcccctacc gcgacggttt tgcgtcatgt aacgccggaa tgatgcatgc ctgtggtcat
    38341 gatgggcata ccaccattgg gcttgggctg gcgcataccc ttaaacaatt cgagtccgga
    38401 ctacatggcg tcatcaaact gatttttcag cctgcagagg aaggtacgcg tggcgcgcgg
    38461 gcgatggtcg atgcaggtgt cgtagatgat gttgattatt ttactgccgt gcacattggc
    38521 actggcgtac ctgcgggcac cgtggtgtgc ggcagtgata attttatggc aaccaccaaa
    38581 tttgacgcgc acttcaccgg taccgccgct cacgcaggcg caaaaccaga agacggttac
    38641 aatgccttgt tggcggcagc acaagccact cttgcactgc atgcaatcgc cccgcacagc
    38701 gaaggagctt ccagagtaaa cgtgggcgtt atgcaggcag gaagcggtcg taacgttgtt
    38761 cctgcctcgg cgttgctgaa agtggaaaca cgcggggcca gcgacgtcat taatcaatat
    38821 gtttttgacc gtgctcagca agcgattcag ggcgcagcaa ccatgtatgg tgtcggcgtt
    38881 gaaactcgtc tgatgggtgc agctaccgcc agttctcctt cgccgcaatg ggtcgcatgg
    38941 ttgcaaagtc aggcggctca ggtcgcgggg gtcaatcagg ccattgaacg tgttgaagcg
    39001 cctgcgggtt ccgaagatgc cacattaatg atggcccgcg tgcagcaaca tcaagggcaa
    39061 gcctcctacg tggtgtttgg cacacagctg gcggcaggtc atcacaacga aaaattcgat
    39121 tttgacgagc aggttctcgc tattgccgtc gaaacgctgg cgcgcaccgc gctcaatttt
    39181 ccctggacgc gaggtatctg atgcaggaaa tctatcgttt tatcgacgat gcgattgaag
    39241 ccgatcgcca acgttatacc gatattgccg atcaaatctg ggatcatcca gaaacacgtt
    39301 ttgaagagtt ctggtcagcg gagcatctgg cttcggcgct ggaatctgca ggcttcaccg
    39361 ttacccgcaa cgtaggcaat atcccaaatg cctttattgc ttcgtttggt caaggcaaac
    39421 cggttatcgc cctgctggga gaatatgacg ccctggcagg tttaagtcag caagcaggtt
    39481 gcgcgcaacc tacatccgtg acgcccggtg aaaatggtca cggttgcgga cacaatttgc
    39541 tgggaaccgc cgcctttgcc gctgcaatag ccatcaagaa atggctggaa caatatgggc
    39601 aaggcggcac agtgcgcttt tatggttgtc ctggcgaaga aggcggctcg ggtaaaacgt
    39661 tcatggtccg cgagggggta tttgatgatg tggatgcggc actcacctgg cacccggaag
    39721 cctttgccgg tatgttcaat acccgtacgc tggcaaacat tcaggcatca tggcgcttta
    39781 aagggatcgc agcacatgcc gcgaattccc ctcatttggg acgcagcgcc cttgatgccg
    39841 taacgttgat gaccactggc accaacttcc tcaacgaaca tattattgaa aaagcgcgcg
    39901 tacactatgc catcacaaat agtggcggga tctcgcccaa cgtggtccag gcgcaggcag
    39961 aagtgcttta tcttatccgc gcccccgaaa tgaccgacgt gcagcatatt tatgatcggg
    40021 tcgccaaaat cgccgaaggt gcggcattga tgaccgaaac cacggttgaa tgccgcttcg
    40081 acaaagcctg ttccagttat ctcccgaatc gcaccttaga aaatgccatg taccaggccc
    40141 tatcccattt tggtaccccg gaatggaact ccgaagaact ggcttttgcg aaacaaattc
    40201 aggctacgct tacctccaac gatcggcaaa acagtctgaa taatatcgcc gcaaccggtg
    40261 gcgaaaacgg caaggttttt gcactacgtc atcgtgaaac ggtactggcg aatgaagtcg
    40321 ctccatatgc cgccaccgat aacgtgcttg cggcatcgac tgatgtcggc gacgtcagtt
    40381 ggaaactgcc tgttgcccag tgtttcagcc cctgttttgc cgtcggtaca cccctacata
    40441 cgtggcaact ggttagccag gggcgaacat ctattgctca taaaggaatg ctgctggcgg
    40501 cgaaaactat ggcagcaacc acactcaatc tcttcattga ttcagggcta ttgcaagaat
    40561 gccaacaaga gcatcagcaa gttacggaca cgcaaccgta tcactgccct atcccgaaaa
    40621 acgtgacacc gtcaccttta aaataacaac aacaacgcaa acacaacaac cgaggaatgc
    40681 ccatgagtat gtcatccata ccgtcgtcct cccaatccgg gaagctctat ggctgggtcg
    40741 aaagaattgg taacaaggtt ccccatcctt ttttgctctt tatctatttg attatcgtac
    40801 tcatggtgac gacggcaatt ttgtcggcct ttggcgtcag tgcgaaaaac ccgaccgatg
    40861 gtacgccggt ggtggtgaaa aacctgctca gtgtggaagg attacactgg tttttaccca
    40921 atgttattaa aaactttagc ggttttgctc cacttggtgc gatcctggcg ctggttttag
    40981 gtgccggtct ggcggagcgc gtcggcttac tgccagcact aatggttaaa atggcatcgc
    41041 atgttaatgc ccgctacgcc agttatatgg tgctgtttat tgcttttttc agccacattt
    41101 cttccgatgc ggcgttagtg atcatgccac cgatgggtgc gctgattttt ctggcggtgg
    41161 gcaggcatcc agttgcaggt ttactggctg ccattgcagg cgtaggttgc ggctttacgg
    41221 ctaatttact gattgtcaca accgacgtgt tgctgtcggg gatcagcacg gaagcggcag
    41281 ctgcgttcaa tccgcaaatg cacgtcagtg taattgataa ctggtatttt atggccagct
    41341 ccgtagtcgt actgacgatt gttggcggcc tgataaccga caaaatcatc gagccacggt
    41401 taggtcaatg gcagggaaac agcgatgaga aactgcagac attgaccgaa agtcagcgtt
    41461 ttggtttacg catagcaggt gtcgtatcgc tactttttat tgctgcgatt gcgctgatgg
    41521 tgatcccgga aaacgggata ttgcgcgatc cgattaatca caccgtgatg ccatcaccct
    41581 ttattaaagg tatcgtgcca ctgatcattc tttttttctt tgttgtctcg ctggcttatg
    41641 gcatcgctac ccgcacaatt cgacgtcagg cggatttacc gcatttaatg attgaaccga
    41701 tgaaagagat ggcgggattt atcgtgatgg tttttcccct cgcccagttt gtcgccatgt
    41761 ttaactggag caacatgggg aaattcatcg ccgtggggct gaccgatatc ctggaaagtt
    41821 cagggcttag cggcatcccg gcgtttgtcg gtctggcgtt gctttcctct ttcttatgca
    41881 tgtttatcgc cagcggttcc gcaatctggt cgattctggc ccccattttc gtaccaatgt
    41941 ttatgctact tggctttcac ccggcatttg cgcaaatcct ctttcgtatt gccgactcat
    42001 ccgtattgcc tttagcgcca gtatctcctt ttgttccact gtttcttgga ttcctgcaac
    42061 gctacaaacc agacgcgaaa ctgggtactt actattcgtt agtcttgccc tatccgctta
    42121 tctttttggt ggtatggctg ctgatgttgc tggcgtggta tcttgtgggc ctgccgatag
    42181 gtccgggtat ttacccacgt ttgtcttaag agagaacgga tgctgagatt acttgaagaa
    42241 aaaattgcca caccactggg tccactgtgg gtgatttgcg atgagcaatt tcgcctgcgg
    42301 gcggttgaat gggaagagta cagcgaacgc atggtgcagc tgctggacat ccattatcgc
    42361 aaagaaggct atgagcgcat ttctgccacc aatccaggcg gtttaagcga caagcttcgt
    42421 gaatattttg ccggtaatct tagcattatt gatacgcttc ccactgctac gggggggacg
    42481 ccatttcagc gcgaagtctg gaaaacacta cggactatcc cctgcgggca ggtaatgcat
    42541 tacggcgaac tggctgagca attgggccgt cctggcgcgg cgcgtgccgt tggtgcggca
    42601 aacggatcga atcccatcag catcgtcgta ccttgccatc gggttattgg ccgaaacggc
    42661 accatgaccg gatatgcagg cggagttcag cgaaaagagt ggttattgcg ccacgaaggt
    42721 tatcttttgc tgtaaacatt aaacaatttg tgccagcttg ttcacacttt tatgtaaagt
    42781 tacccttaac aacttaaggg ttttcaaata gatagacata tatttacatc taatatcgga
    42841 attctctgct gttaaggttt gcttagactt acttgctccc taaaaagatg ttaaaattga
    42901 caaatatcaa ttacggcttg agcagaccta tgatcccgga aaagcgaatt atacggcgca
    42961 ttcagtctgg cggttgtgct atccattgcc aggattgcag catcagccag ctttgcatcc
    43021 cgttcacact caacgaacat gagcttgatc agcttgataa tatcattgag cggaagaagc
    43081 ctattcagaa aggccagacg ctgtttaagg ctggtgatga acttaaatcg ctttatgcca
    43141 tccgctccgg tacgattaaa agttatacca tcactgagca aggcgacgag caaatcactg
    43201 gtttccattt agcaggcgac ctggtgggat ttgacgccat cggcagcggc catcacccga
    43261 gcttcgcgca ggcgctggaa acctcgatgg tatgtgaaat cccgttcgaa acgctggacg
    43321 atttgtccgg taaaatgccg aatctgcgtc agcagatgat gcgtctgatg agcggtgaaa
    43381 tcaaaggcga tcaggacatg atcctgctgt tgtcaaagaa aaatgccgag gaacgtctgg
    43441 ctgcattcat ctacaacctg tcccgtcgtt ttgcccaacg cggcttctcc cctcgtgaat
    43501 tccgcctgac gatgactcgt ggcgatatcg gtaactatct gggcctgacg gtagaaacca
    43561 tcagccgtct gctgggtcgc ttccagaaaa gcggtatgct ggcagtcaaa ggtaaataca
    43621 tcaccatcga aaataacgat gcgctggccc agcttgctgg tcatacgcgt aacgttgcct
    43681 gatttttccg cataactcac tatccttctg tcatatcatt aaatttttct gatttattga
    43741 tctggcagaa ggttcatcac tgtttcattc accagatatg ggttaatctt ttaattacaa
    43801 actgcgttga cagttgttgt aaggagaccc tgtatggcta tgtatcagaa catgctcgtt
    43861 gttatcgatc ctaaccagga cgaccaacca gcattgcggc gagctgttta tttacatcaa
    43921 cggattggtg gcaaaattaa agcctttttg ccgatctatg acttctcata cgaaatgacc
    43981 accctgctct ccccggacga acgtaccgct atgcgtcagg gcgtcatcag ccagcgtaca
    44041 gcctggatcc acgagcaggc aaaatattat ctcaatgctg gcgttcccat tgaaattaaa
    44101 gtggtctggc ataaccgtcc tttcgaagcc atcattcagg aagtgatcag cggcgggcac
    44161 gatttggtgc taaaaatggc gcaccaacat gaccgtctgg aagcggtgat ttttacgcca
    44221 acggactggc acctgttacg caaatgccca agcccggtgt ggatggtgaa agaccagccg
    44281 tggccggaag gaggtaaggc gctggtggcg gtgaatctcg ccagtgaaga gccgtatcat
    44341 aatgcgctca atgaaaaact ggtcaaagag acgatcgaac tggcagaaca agtcaaccat
    44401 accgaagttc atctggttgg cgcttatccg gtaacgccaa ttaatatcgc gattgaactg
    44461 ccggaatttg atccgagcgt ttataacgat gccattcgtg ggcaacattt gctggcaatg
    44521 aaagccctgc ggcagaaatt cggcattaat gaaaacatga cgcacgtaga aaaaggtctg
    44581 ccagaagagg tgattcctga tttggcggag cacttacagg cggggattgt ggttctgggc
    44641 acggtaggac gcaccggtat ttcagcagca ttcctcggca acacggcgga acaggtgatt
    44701 gatcatcttc gctgcgatct gttggtcatt aaacctgacc agtatcagac acccgttgaa
    44761 ctggatgacg aagaagacga ttaacgactc ctcaataact gacaaggccc gagttatcgg
    44821 gcctattttt tacttactgc tcttcgccgc caaggaaata aattcccaac gggattgcca
    44881 gcaacacagt aaagaccagg ctataaacaa aaatcatcga tgactgaatc acatacattg
    44941 atgtcgtcca ctccgacagc ggaatattgt actgttctat aacgccgccg atagtcgcgc
    45001 gagtcacaac cgacgccgca ataatgaaaa ccgccaacaa accaaagaga aatttcttcc
    45061 ctttcgctga cttcagtttc gccataatca tgatgtaatc cttgtgcaga caaatttctt
    45121 gttacctaaa cgtaacaata ccatgacatt tcaccattgt ctgctctgaa aacctcacct
    45181 cttagctgga tttacaatca acgcattgat tagttagcta atgttgctac ttccttccct
    45241 gactattgat gatctgaatg aattgtcgcg gtaaaatagc cccgccaggg gtttccggca
    45301 tgacgacaat tatctggagg agcctgataa aacaatgatc gctgaactgt ttacaaataa
    45361 tgcgcttaat ctggtcatta ttttcggtag ctgcgcagca ttgattctga tgagcttttg
    45421 gtttcgccgc ggaaatcgta aaagaaaagg ctttttattc catgcggtgc aatttttaat
    45481 ctacaccata attatcagtg ctgttggtag cattattaat tatgttatag aaaactataa
    45541 actcaaattt atcactccag gcgttatcga ttttatctgt acgtccctga ttgcggttat
    45601 tctgacgatt aagctatttt tgctgattaa tcagtttgaa aaacagcaga ttaaaaaggg
    45661 tcgcgatatc accagtgcgc ggattatgtc gcgcattatc aaaatcacca ttattgtggt
    45721 gcttgttctg ctttatggcg aacatttcgg catgagcctt tctggcttgc tgacctttgg
    45781 tggtattggt ggtctggctg tcggtatggc tggcaaagat attctgagta atttcttttc
    45841 cgggatcatg ctctatttcg accgtccttt cagtattggc gactggatcc gttcaccgga
    45901 cagaaatatc gaaggtacag tagcggaaat tggctggcga attaccaaaa ttacgacctt
    45961 tgataatcgt ccattgtacg taccgaactc gctgttttca tcgatcagcg tagaaaaccc
    46021 aggacgaatg accaaccgcc gcattaccac gaccattggt ttacgttatg aggatgcggc
    46081 aaaagtgggc gttatcgtcg aagccgtacg tgagatgctg aaaaatcacc cggccatcga
    46141 ccagcgacaa accttactgg tttatttcaa ccagtttgct gactcgtcat tgaatattat
    46201 ggtttattgc tttaccaaaa ccacggtatg ggctgagtgg cttgctgcac agcaagacgt
    46261 ttatttgaag attatcgata ttgtacagtc acacggcgcg gactttgcct tccccagcca
    46321 gacgctgtat atggataaca ttacaccgcc ggaacagggt cgctaaatca gacatcactg
    46381 gcgttatcca caagggtaac gccagttccc atcacatcaa tgcttcacaa tatacatagt
    46441 ccgactgtac gccacatcct cagggttatt aatgggataa cctttcagcc acggtttgat
    46501 taatcgtcca ttggtatatt gataaattgg tgcaatcggt gcttgctcca tgaggatttt
    46561 ttctgccgcg ttgtaatcgg cattacgcgc tttaacggta ttttccgtcg atgcctgggc
    46621 cagaactttg tcgtatgccg gattgttaaa gcgtgaaata tttcctgaat gcgttgacgt
    46681 taataatgtc aggaaagtgg agggttcatt ataatccccc acccatgagg cgcggataac
    46741 atcaaaattg ccggtgttac ggctatcgat ataggttttc cattcctgat tttgcaattt
    46801 aacatctacg ccaaggttct ttttccacat cgatgctaca gcaatcgcaa ttttttgatg
    46861 gttttctgaa gtgttataca aaagcgtcag cttcagcggt ttttgcggac cataaccagc
    46921 tgcgctcaac aaagtttttg cctgcgcatt cagttcttcc tgactcattt gttcaaacgg
    46981 cgaaggttcc ggcgtaaatc cggcggtaac atctggtgta aaatgccacg ctggcttttc
    47041 gcccgtccct aatacttttt cggtcatcag gcggcgatct atcgtcatac ttaatgccag
    47101 acgaacgcgc tgatctgccg tcggcccttt ttgcgtgtta aacgcataat aataggtccc
    47161 gagctgcggc ggcgtataaa cctgccccgg aatatccttc aacagcttct gatacatatt
    47221 tttcgggaag gattcggtga tatcaatatc ccccgcgagg taacgcttag tggctgcgga
    47281 ttcctgatta attggcagga aggtcacttt ttgcagtacc gttttggcgt tatcccaata
    47341 atgggtattc ggtaccacga ccagtttttc attgactacg cgctctttaa gaacataagc
    47401 gccattgccg atcagatttc cgggtttcgt ccactcttta ccgctttcta cgttggcttt
    47461 ttgcaccggg aagaaggcaa agttagcggt taaattcaca aaccacggca acggtttatc
    47521 aagctgaatt ttcaaagtat ggacatcaac tgcggtgacg ccaagctggt caggtgtagc
    47581 tttaccatca ataatcgcct gtgcgttgtt gattcccgcc agcgcggcaa accatgcaaa
    47641 tggcgatagc gttttcgggt ccaccagacg ctgccagctg tagacaaaat cttgcgccgt
    47701 taccggtgtg ccatccgccc attttgcgtt atcgcgcagg gtaaaagtcc agatacggtt
    47761 gtcattactt ttccactgag tcgcaacgcc ggggacaatc tcccctttct cgttctgatt
    47821 caccagtcct tcaaacagat cgcgaatgac ctgaatctct ggcaggccca cggctttagc
    47881 gggatccagc gacgcaggct catctttaat gtggcgcacc agctcctgct tctctgccag
    47941 tactgtgccg ctcggaactt ctgcagcata cgaaagagaa atgctgctga ccaacagcgc
    48001 acaacacgtg actgaaacag agtgcttcat aagtttccct ctaaccgaca ttaaaattga
    48061 tgcgtaatta tttgtttcgc cttaaagaaa tgcaaacaac ttccatcaga aagtgacaca
    48121 ggaagcataa aatgttttat gtatcaatat attaaatcag atttagtgta aataatagac
    48181 gccaaaatgc ggcaaagcac atagaacacc gaacactacg ttcgggcttc acacgttcaa
    48241 accagttttc ggtatcataa tcagcatctg tcgccattgt acgaagaata aaaaataacc
    48301 aaaacgttga aaggaactga gaatacgtta agagaagaat taaattaatg tatcagcaag
    48361 tagtgctaaa cagcaccttt atacttcagc ctatctataa ataatgagaa tgccgagcca
    48421 gcatgtcgac ggtgtggata gtacaggtga taaccgggta aatctggtgt gaatttatcc
    48481 aaaacacgta tcagtttttt ttctttaatc gcccgctcaa cctgatcata aggtagatac
    48541 gccaatccat gcccatcaat tgcagcatca atgatcaggt ctatcgtatt cagtaaaagc
    48601 tgaccttcca tgcgaacacg aacttcacgt ccaccgcgta ttaatctcca gcgatttgct
    48661 gtacccgatg tgggaagata caaattaatt gcctgatgat ctattaattg tgacactgac
    48721 gttggaacac ttcggcgaga aaaataatcc ggtgagccaa caatagccat tggtatatct
    48781 ggcccgattc gaatggcgat catatcttta tccatctccc cacccagacg gacgcctgca
    48841 tcaaaacgtt cagaaacgac atcagtcaaa ccataatcaa tgataagctg aatatcaatt
    48901 tcagggtgtg atttcaggaa tgtgcgcatg gctggtaaca atatcgtttt tgctgcatgt
    48961 tctacagtag taatacgtat tgtcccggat gggcggttct gcagatcgct cagggatgcc
    49021 agggcagaat ctatgtcatg cagcataggg ccaagaacag acaaaagatg ctcgcccgct
    49081 tcagtgggta caacactgcg cgtggttcgc gtcagaagcc gcaatcccaa tcgttcttct
    49141 atacggcgca ctatctggct taaagctgac tgcgccatgc tcaggcgggc tgctgcgcga
    49201 gtgaagctac gctcctctgc aacgacgaca aacgccatca gatcagcgat ctcttcacgc
    49261 ttcattcagt atctccatac aatctattaa tcatttaaat gataggtcta atcatgattt
    49321 agggtctaat caacttaatg atcaatatct atagtgatat caccaaccgt taaaacgaac
    49381 caacaactcc atgaggaacg gaatgatgaa taataaagtc agcttcacta acagcaataa
    49441 tccaaccatc tctttgtctg cagtgatcta ttttcctcca aaatttgatg aaacccgtca
    49501 atatccggca attgtggtat ctcacccagg tgggggcgtt aaagaacaaa cggccgggac
    49561 ctatgccgaa aaactggcgg aaaagggatt tgtcactatt gcttatgacg catcttatca
    49621 gggtgaaagt ggcggcgagc cgcgtcagtt agaaaaccca tacattcgta ctgaagacgt
    49681 cagtgccgtg attgattacc tgacaacgct ttcttatgtc gacaataccc gaattggtgc
    49741 gatgggaatt tgtgccggtg caggatatac agccaatgct gcaattcagg atcgccgcat
    49801 caaggccatc ggtaccgtta gtgcggtcaa tattggttcg atgttccgta atggttggga
    49861 aaacaatgtg aaatctattg atgccctgcc atacgttgaa gcgggttcaa acgccagaac
    49921 cagcgatatt agcagcggag aatacgccat tatgccactg gcaccaatga aggagtccga
    49981 tgcgccgaac gaagaactgc gtcaggcctg ggagtattac cacacccctc gcgcgcagta
    50041 tccaacagca ccgggttacg ctactttgcg cagccttaac cagattatta cctatgatgc
    50101 ttaccatatg gcggaagtgt acctgactca gccgacgcag attgtggcgg gcagccaggc
    50161 agggagcaaa tggatgagtg acgacctgta cgatcgggca tcaagccagg ataaacgcta
    50221 tcacattgtt gaaggcgcaa accatatgga tctttatgac ggcaaagcct atgtggctga
    50281 agcaatttca gtattagctc cgttctttga ggaaactctg taatgaaaca tattcaaatc
    50341 cgcaatagtg atatggcctg gcatattgct gccaatattc agtttccacc aaattttgat
    50401 gagtcaaaac agtatccggc gattatcagc gtccatcctt ttggcagttg taaggagcag
    50461 acgtctggca atatctatgg caaggcattg gctgaaaaag gctatgtcgt tctcgcttat
    50521 gacgccagtt tccagggaga atctggtggg gagcctcgtt ggatagaaga tccgacacag
    50581 cgcgtagagg atattagccg tgttatcgat tatgcagtaa ctctgccata tgttgatgct
    50641 gaacgcattg gcgtgctggg tgtgtgtggg ggggggtggc tatgccatta atgccgcgct
    50701 gacagaaaaa cgaattaaag ctgtagtgag tattacgggt gttaatatcg ggcgtctttt
    50761 tcgggaaggt ttcagcaatt acgatccgat tggcgcattg aatgcaatgg cgtcgcaacg
    50821 aaccaaagag gctcgtggtg gagaattaca gatcaacgaa ttacttcccg ccagcctgga
    50881 tgcagcaaaa gcccatggac tgaccgaacg cgatgtttat gaagcaactg actactataa
    50941 aacaccacgc gggcaacaac cgggaggtgc gacaaaaatg ttgttctcac atgcacagaa
    51001 aacgctggcc tgggatgcct tcgcatttac tgaagtgtta ctgactcagc cggtgatggt
    51061 agtagttggt gaaaaagtcg gtgccttcgg tgcttatcgt gatggactgg aagtctatgg
    51121 ccgggcgatg gtttcgcagg atcgtcagtt agtttcatta cccgatttct cacattatga
    51181 actgtatgac aaaccggaag cagttcagga ggcgctggaa aaagttattc cttttttcaa
    51241 cacccatctg tgataagttt catgagaaat attatgaaaa atgtactcat tcttggtgct
    51301 ggcggtcaga tagcccgcca tgtgattaat caactggcag ataaacaaac cattaaacaa
    51361 actttatttg ctcgtcagcc agcaaaaata cacaaaccct atccaactaa tagccagatt
    51421 attatggggg atgtactgaa tcatgcagca ctgaagcagg ccatgcaggg acaggatatt
    51481 gtgtatgcca accttacggg ggaagattta gatattcagg caaattcagt gatcgctgcg
    51541 atgaaagcct gtgatgttaa acgtctgatc tttgttcttt ccctcggcat ttatgatgaa
    51601 gttccaggaa aatttggcga atggaataat gcagttattg gtgagcccct aaaaccattc
    51661 cgcagggctg cggatgcaat tgaggcttct ggactggaat atacaattct tcgccctgcc
    51721 tggctgacgg atgaagatat tattgattat gagcttacct cacgcaatga accattcaaa
    51781 ggtacgatcg tatcacctaa gagtgtcgca gcattaatta cagatatcat cgataaacct
    51841 gagaaacaca ttggtgagaa tataggcatc aatcaacccg ggactgatgg cgacaagcca
    51901 ttcttcatgt aaatcaatta tataaccacc atcgtactgt actatgtgca gtacgattta
    51961 ttaaacttat taaggtgaac tgatcaatca gatgcgatta acatgtgtag aaccagtaag
    52021 aataaaatac gccatgtata taacattacg acaattctaa tatctgcaca tcaatgaaaa
    52081 tacaaacaac ttccggcaaa aaagtgatac aagatcgatg acatagcgtt gaagaaaagg
    52141 atttgataat ccgcaaaatt tgcacaacac tgcctgtaat acttcctgaa ccagagacga
    52201 tcatgaccgt aacccgccca cgcgccgaac gcggcgcatt tccgcccgga acagaacatt
    52261 acggacgttc attattgggc gcgccgttaa tctggtttcc ggcccctgca gccagtcgcg
    52321 aaagtggttt gattctggct ggcactcacg gtgatgaaaa ctcttcggtc gtcacgctct
    52381 cctgtgctct gcggactttg acaccttctt tgcgccgcca tcatgtggtg ctgtgtgtga
    52441 atcctgacgg ctgccagctg gggttacggg ccaatgctaa tggtgtggat ttaaacagaa
    52501 actttccggc ggcgaactgg aaggaaggtg aaacggttta tcgctggaac agcgccgctg
    52561 aagaacgtga tgtcgtatta ctgacaggtg acaaacctgg ctccgaacct gaaacccagg
    52621 cgctgtgcca gcttatacac cgcattcaac ctgcctgggt ggtctcattc cacgatccac
    52681 tggcctgtat tgaagatccc agacacagcg aattaggcga atggctggct caggcgtttg
    52741 aattgcctct ggtaaccagc gtcggttatg aaacgcctgg ctctttcggc agttggtgtg
    52801 cagatctgaa cttgcattgc atcaccgcag agtttccgcc catctcctcc gacgaagcca
    52861 gcgaaaaata tctctttgcg atggcaaact tgctgcgctg gcatcctaaa gatgcaattc
    52921 gcccgtcgtg aactgaagcg ccggttccac atctaccgcc agccaggtcg gtccgtcaag
    52981 atcggcgaaa ctgacctgcg gcaccagcgg taaagcggcg ctaatggccc gagaggtaca
    53041 caacatgcag cccagcatca gactgaaacc ttgtgcacgc gcttcagtcg ccagcgccag
    53101 cgcttccgtc agacccccgg ttttatcgag cttaatatta accatctcat agcgcccttt
    53161 cagcgccttc aaattgctac gagtatgaca actttcatca gcacaaatcg gcaacggatg
    53221 aataaaattc tccagcgccg catcgtcctg cgcaggaagc ggttgttcaa gcatcgcaac
    53281 gcctaaatcc gccaatagct ggcaacgtgc cgccaaccct tctgcatgcc aggattcatt
    53341 tgcatcaacg atcagcgtgg tatcgggcac agctgtgcga attgccacca tccgctcact
    53401 gataagatgg ttatccagct tcactttcag taatttcgcg ccggcctgcc agagtgttga
    53461 tgcactattg gccatctgat caggcgtacc gatgactacc gtctgtgcag tgataactgt
    53521 ctcgggaagc gttatgccga tcaaatcagc cagcgattgc tgctgttttc gcgcggccag
    53581 atcccacaat gcacaatcca gcgcattacg tgctgcgccg gcagggagaa ttttttgcaa
    53641 ctcctcccgt gtcagccctt tctctaattg cggcacgacg ctcataattt gcgccattac
    53701 cgaggcatca ctttctccat aacgcggata cggcgtgcat tcgccggtgc ctttaatacc
    53761 ctcttcttcc agttcaacca ccaccacgcg cgcttcactg cgacttcccc gggcaatcac
    53821 aaacggggta tgtaagggcc aggcttcctc aaatacctta acggttctca tctttactcc
    53881 ttggtgtcgc ggtggtctgc ggattaagcg caaatagcgt ttgctgtgtt attgacagtt
    53941 agcataaact aggtgtgacg ttaactatat gtaaacagga aagataatca tgtcacaaac
    54001 cgttcatttc cagggcaacc cggttacagt cgccaattcc atcccgcagg cgggtagcaa
    54061 agcgcagact tttactctcg tggcaaaaga tctgtctgac gtcaccctcg gtcagtttgc
    54121 gggtaaacgc aaagtgctga acattttccc gagtattgat accggtgttt gcgccgcatc
    54181 agtacgtaag tttaaccaac tggcaaccga gatcgacaac accgttgtgc tgtgtatctc
    54241 tgccgatctg ccgttcgccc agtctcgttt ctgcggcgca gaaggtctga acaacgttat
    54301 caccctctcc actttccgta acgctgaatt tctgcaagcc tacggtgtgg caattgctga
    54361 tggcccactg aaaggtctgg cagcgcgtgc cgttgtggtt attgatgaaa atgacaatgt
    54421 gattttcagc cagctggtgg atgaaatcac caccgagccg gattacgaag cagctctggc
    54481 tgtactgaaa gcataagtta atatgcctga tggtgttgca ccatcaggca tattcgcgct
    54541 tactcttcgt tcttcttctg gcttaagcca tattcccgca acttattggc aatcgcggta
    54601 tgtgaaacgc cgagacgttt tgccagtttg cgcgtgctgg gataattgcg ataaagctgg
    54661 gttaataccg agcgttcaaa acggctggtg atttcgtcca gcgaaccttc catcgcatct
    54721 tcgcccacgg ctaccgttgc ggcgtcataa tccggcaaca aaatatcctg tggacgcagc
    54781 tcataaccgt ccagttgtgt cagtgcgcga tagatagcgt tctttaactg ccgcacattt
    54841 cccggccacg cataacgcgt aagtacagta ttcaggtcag cggccagttt cggacgcggc
    54901 acgccctgct cgtcggcaaa gcgggcgacg aacagctcag ttaacggcat gatgtcctgc
    54961 ggacagtcac gtagcggcgg caggttgagc gtcaacacgt tcagacgata atagagatct
    55021 tcacggaaca tgcctttttg caccagttcg accagattct tctgcgtagc gcaaatcacc
    55081 cgcacatcga catgcacctc atggtcttcg ccaacccgac ggaaagtgcc atcattaagg
    55141 aaacgcagta atttcgcctg catccgtggt gacatctcac ctatttcatc caacagcacc
    55201 gaaccaccgt tcgcctgctc aaagaatcct ttcttccctt ccggagcatg accaaacagc
    55261 tcactctcga ccgcatcttc cggtatagac gcacagttca gcgccaggta aggtttgctc
    55321 gctcttgggc ttgcctgatg gcaggcgtag gcaaaaagat ctttacctgt acctgtgtca
    55381 cccgtaatca gcagcggcgc gcttagcatc gccagtttct gcgcctgttc gacaacatgc
    55441 ttcattttcg ggctgacagc gacaatttga ctgaaggcgc tgacgtcctg ggcggcgaca
    55501 ttttgcaact gacggcccat acgaatcgtt gatcgcaaca tcaccaccgc acctgtcagg
    55561 acgtgttgat cattttcatc ctgaagataa acaggcgtaa tctccatcag gaagttctgt
    55621 ccattaataa caacatgctc gttatgggaa tcttgcggtt cgctttccag ccaacgtaaa
    55681 aaattaaagc cgttaatcaa ttgtgcggcg gtatggttgc gcagtcgatc caatttttgc
    55741 ccaaaaagct gacagctcgc cgggttcgcc atatccactt tgcttttcat atcgacagag
    55801 agcacaggtt caggcaacgc ctccagtaac gcgctcaacg ccagatgctc acgttcggaa
    55861 ggcatccacg ggacagtacg cacatcggta acacctgcaa tacggcgtat ttcggccatc
    55921 agactgctga aactctcaaa ctccagttca gcaaaattga ggtagattcg cccaatggga
    55981 tcaatctcaa taccgcgtaa atcaatgcct cttagcacga gtagatcgag taattcgcgg
    56041 gtcagaccga gtcggtcttc acaaaagact tccagacgca tgggaacctt cacctgaaaa
    56101 aagaacaatt aatatgatga tatgacacta tttgaatagc aggaaggttt ctgtcaacaa
    56161 tcattgacac aaagacggaa aaacgggcac ttcggcgtaa agatatccgg tcagcacccg
    56221 atattgcatt atttttcgct gggcgttttg cctttttgca gcgtttcttt cacctgaccg
    56281 ataagctgac gacggaaatc cccgaggcga ggtttgtcat cgtcaatcca cggcagcggg
    56341 cggcaaagct ccatagcttt aatccccagt cgtgccgtca gaagtcctgc accaatcccc
    56401 tgagctgcgc gggtagacaa acgagcagcg agatcttgcg acatccagtc cattcccact
    56461 tcgcgcacca attcgctggc tccggcgaaa gcgatattca gcaataccag cttgaacagg
    56521 cgcaaacggc tgtaatatcc cagttcaatg ccatacagcg tggcgatgcg attaatcaaa
    56581 cgcagattgc gccaggcgat aaatgccata tccaccagcg ccagcgggct gaccgcaatc
    56641 atcaacgttg attcagctgc tgagcggctg atttcgcgcc gcgcctgggc atctaaaacc
    56701 ggctggacca gatgagcata caggctgacc acttcacgat cgttctgcgt ttcatggatt
    56761 gaggcatacc agcgttgcag cgctggatga gactgatcaa tacccgcctg ctgcgccagt
    56821 ttttcgcaaa atgcgcggcc tttgcccgtg ccgtggctgt gcaacaaatc gcgcgcttcg
    56881 tcgcgttcat gggcgcgctg tcgcaagcgc cataagcgcc gccactctgt taccacagaa
    56941 cctacgccag cgccgataat caatgcccca gcagcacatc cacccagcgc cacccagtcc
    57001 tgagtttgcc aggcattcat tgtccactgt acaccctgcc cgacaacgct tgcgccaaac
    57061 agagccagcc cgcccatcac cattttgcgc cacaggctgc gtttcggacg taacgctgcg
    57121 tccattaccg cttcgacttg cccctcttct tcctgcgctt cgtcgagcgt ggccggggca
    57181 aaattttgcg cctgattttc gtcaaaggtc tgctgcgccc tgaattttgg attctgttcg
    57241 acctccagcg gaccgtcgaa atcaatacgt ggttttaacg gttcggtcat cgcaatttat
    57301 ctcctattaa aaattccagc gcggcatcaa gacgaatatg cggcagcggt ttgtcgacat
    57361 ccatcacctg cgggcgaaac gcttcaaact ggaacccttg cttatcccag aacgcctgac
    57421 caggcaaacg tgcgggaact tcgccaggat aaacagtgag cggtgcgcca tcgctaagtc
    57481 gattaccgcg cagcgccggg attttctcac cgttaacatc aataatgcca ctggtggtcg
    57541 cctgaactga cgccagcccc aggcaatcca tgctgatccc ttcgaacgcc gcattttgcc
    57601 aggcatcctg aattagttgt tgcagcaatg aaaccatatt ggcgtgctga tcgatggtca
    57661 catggtccgc tttagtggca gcaaacaata gcttatcgat aaccggcgaa aacaaacgcc
    57721 tgaacagggt acgctgccca tagtggaaac tttgcatcag ctgcgtcagc gccagacgca
    57781 tatcattaaa tgcctgtggc ccactgttga gaggttgcag gcaatccacc agcacaatct
    57841 ggcggtcaaa gcgcagaaaa tgattcttat agaacccctt caccaccttc tcgcagtaat
    57901 aattaaaccg ctcgcgcagc attccggcat tggtgtgctt atcggcctgc gccagtttgg
    57961 actcgcccca ggcatcgaca tccggccacg ggaagaattg cagcgcgggc gcacctgcca
    58021 tatcccccgg caagacaaag cgcccaggct gaataaagtg cagcccctgc tgtttacagt
    58081 ggtggagata atcggtccac gcggcggcaa tgtctgccag ccggttttcg tcggcaggcg
    58141 ctagcgggtc cagcccttcg cacatcattc gccatttcgc cgaccattcg ccgcgctgac
    58201 cattgagtaa gcccgtcatc tggcgcgacc agcttaaata gtcctgcgcc agcatcggca
    58261 ggtcgagcaa ccattcgcca gggtaatcca caatctccag atacagcgtg gaggtatcct
    58321 taaagtggcg cagcagcgaa tcgttcgatt tatagcgtag tgccaggcga atttcgctga
    58381 cgccgcgcgt tggcgtcggc caggcgggag gatcgccata cagctgcgcc aacccttcgt
    58441 cgtaggtaaa acgcggaatg ccaaagtcac gctgaggaat gcgttttacg cccagcagac
    58501 gctcttcacg caccgcactt aatagcggca aacgtgctcc ggcatgaata ttgagcaact
    58561 gattgaccat cgcagtgata aacgccgttt tgccgctgcg gctaagtccg gttacggcga
    58621 ggcgcagatg tctgtcgaca ccccgattca ccagcgcatt aagttcattt ttaagtcgct
    58681 tcattgctgt cctgtgctgc tctgaagacc agaaaaaaat cattcataag gatatgaatg
    58741 tgggggccag gccattgatt atcaactacc cgtgttgtca tcgacgggcg aactccataa
    58801 gatgtgcagc attcacgttc ggacggagtt cctgacctat gtcccctact atttatgata
    58861 ttgccagggt tgcaggcgta tcaaaatcca ccgtatcacg cgtgctgaat aagcaaacca
    58921 atatctcccc ggaagcgcgc gaaaaagtgt tacgggccat tgaagaatta cagtatcaac
    58981 caaacaagct ggcccgcgcg ctgacctctt cgggttttga tgccattatg gtgatttcta
    59041 cccgttcgac caaaactacg gcgggtaatc cgtttttctc tgaagttttg catgccatca
    59101 ccgccaaagc tgaagaagaa ggtttcgacg tgatattgca gacgtcgcac accccggcag
    59161 aagacttaca aaaatgtgaa agcaaaatta agcagaaaat gattaaaggc attattatgc
    59221 taagttcgcc agcggatgag tcattttttg cccaacttga taaatacgat attcctgtgg
    59281 tggtgattgg caaagttgaa ggtcaatatg cccatgttta ttctgtcgat accgataatt
    59341 ttggtgacag cattgcgttg accgatgcgt taattgaaag tggacatcaa aatattgcct
    59401 gtctgcatgc accgcttgat gtccatgttt cagtggatcg ggtaaatggt tataagcaga
    59461 gtctggaaga tcataatatt gcagtgcgtg atgaatggat tgttgatggc ggttataccc
    59521 atgaaacagc actgcaagca gcacggcaat tattaagtca gtcaccgttg cctgaggcag
    59581 tgtttgccac tgacagcctg aaattaatga gcatttatcg tgcggcggca gagaaaaaca
    59641 ttgctattcc gcagcagtta gcggtggtgg gttatagcaa tgaaacgctg tcatttattt
    59701 taacgcctgc accgggcggc atcgatgttc cgacgcagga gttaggtcga caaagctgtg
    59761 agttattatt ccgcttaatt gccggaaaac cgtcaccaca aaatattacc gttgccacgc
    59821 atatgacgct gaaataaata ccagcgacct tgcctgaatg ccggatgcga tgctggcgca
    59881 tcttatccgg cctacattta tagatcggat aaagtactcg cgccgcatcc ggcaatttag
    59941 cccattatca gaacgagtaa tttacgccga cacctgcata atggaattta tcgctgtcgc
    60001 cttcgtcgtg atcctgccac tcaaacgcgt attccagcga aatggaaaga ccattctgga
    60061 aatcataacc gtaaaataaa ccaacacggt taaaatcatg gccttcacgt tcaatatcat
    60121 cctgccagtc ccagttactc cagcgatcca gcccaatgcg cgtatacggc gtcaccgagt
    60181 ggttgccgag cgtcagcggc aaataggcgc gaatttcttg cgtggaaaac tcaccgttat
    60241 tacggctgtc gtccatattg aagccgcgct cgagataata gttcactcgc aaggcaaccg
    60301 tttcgttgaa ggtatattgc agacctgttt ccgtttcgac acgggtatca gcgtaaccgg
    60361 tcgtgttcaa atcgttggca aatttataca tcgacaacca accgttgaaa cgtaaatcgt
    60421 cagtcagttt cacatcccag tctggcgcga ttttccagcg ctgcatattc gccgtgtctt
    60481 tacccggttc atcaacgtag tgataaccat aattacggaa accgccggtc aggccgaaac
    60541 tgaaatcatc gctttcgagg aactgataat gtacctccag ctccgggcga tcaaaccacg
    60601 ttccacgttt acccgcgcta taatctaccg gcccttcctg ataataggcc agagaaattc
    60661 tccacggccc gttggcggca ttaaaataga cagaaggctc cgccagccca tccatatctt
    60721 cgccataacc ctcgacgttt tctatttcgt acatcgcgcc gatattaaag tgccagtcgt
    60781 tcttttcctc ggcctgtgcg caggccattc ccgcacacat caccagtgcg gtacagggta
    60841 ataacttttt cattattgtt ccttgtatta tcctggggga atgccttccc cctgtattta
    60901 gcgaattgct atttccgttt ctgcatcaaa gaaatgacat ttagtcatat caaaatgaat
    60961 agtgatattt tctcctgcat gataatcatt taacgcacca gcacggacca ctaactcgtg
    61021 ccccccaacc gtggtgtaga gcataaattc cgcaccggtt aattctacca cgctaatttt
    61081 ggcggaaata ttattttcct cttgcgcgtc cggatgaata tcttccgggc gcattcccat
    61141 cacgatgggc ttatgcaaac tttcctgtgt tttcagaacc gctaatttct cttcgggaat
    61201 ggttaattta agcgtttccg taacgaattt atcgccatcg atcgtgccgc gaataaaatt
    61261 catcgctggt gatccaataa atccggcaac aaacatattc gcgggttggt tataaacggt
    61321 tttcggcgca cccacttgct gaacaatccc gtctttcata atcacaatcc gcgtcgccat
    61381 ggtcatcgct tcggtctgat cgtgggtcac gtagatcatg gtggtgttca gtttctgatg
    61441 cagcttgctg atctctgcgc gcatttgtac gcgcagcttg gcatcaaggt tagagagcgg
    61501 ttcatccatt aaaaacacgc ccgcttcgcg cacaatcgcc cgcccgagcg ccacacgctg
    61561 gcgttgcccg ccggaaagcg cgcccggctt gcgcttcagg tactcacgca ggccgagaat
    61621 ttgcgccgcc cagttcaccc gctcatcaat cacctctttg gcgatttttt gcatcttcag
    61681 gccaaacgcc atgttgtcgt aaaccgtcat atgcggatac aacgcgtagt tctggaacac
    61741 cattgcgata ttgcgtgctt tggctggaac gtcattcatt cgtttgccgt cgatcaacag
    61801 atcgccgccg ctgatctcct caagcccggc aatcatgcgc agggtggtcg atttaccgca
    61861 gcccgacggg ccgacaaata cgatgaactc tttatcggca atttccaggt tgaagtcctt
    61921 caccacatgc acctggttat cgtagatttt ttgaatatgt tgtaacgaaa gctgagccat
    61981 gttgattcct ttgctatacg ttttgccaga aggccgataa ccgcggccag gtcagggagt
    62041 ccgttgaggg caacaataat tgcgccccgg ttaagcctgc gccgatcccc accgagcgca
    62101 taccgctggc gttaatggcg tcaatgcccg cctgcgcatc ttcaatgccg atacatgcct
    62161 gcggcggcac gcccagcccg gcacaggcag cgagaaagat ttccggatcc ggtttcgagt
    62221 ttttaagttg ggaagcatcc gcgcagaagg tgaaaaactc gcgcagctcc agcgccgcta
    62281 aaatcgtcgg cgcattcagg gagacagaag ccagccctac cgggatctgc tgtgcgcgga
    62341 gctctgccag caaattgcga ataccgggca gcacagcatt aaccgtcagc tcgcgcagtg
    62401 agtggacgta aagcagattt ttgcgatacg ccagttgcgt ccgctcctgc gggttaaagt
    62461 cgccctcttt gcccccgtgt tgcagaatgc gccgcagaga ctcatcgcgg ctgatccctt
    62521 ttagggattc gttaaactgc gcatcaatgc tgatgccaat ttcagcggca atctgctgcc
    62581 acgcctggaa atgcagatgc gcggtatcgg tgattacacc atccagatcg aaaattaccc
    62641 cttgcagttt cattcatcct cctgatgcgt ggtagcggtc ccattgaagg gcaaaataaa
    62701 atcacccaaa cagaaaacag attcttcagc cacggatata agctgcccgt tcaaacgcag
    62761 tgaaacgggc gcagaagttc gaatcgcaat acgctgcgcg tcgagcgtga cctgtaattc
    62821 gcagccctgc cagaacagag ggaaagacaa ctgttgccac tgctccggta acgccggatt
    62881 gagatgtaat tcaccgtcac gcacgctcac cccggcaaaa ccctgaatcg cccccagcca
    62941 gatagcgcca gttgcggcag cgtggatacc atcatcacaa ctatgcggat cagcaccaag
    63001 atcgatttca gtcccctcgc gccagaactg ataactttgg gtcagcaggc cacagcgtgc
    63061 ggcaacaatg ccgtggattg ctttacttaa tgacgagtcg tgaatagtgc gcggttcata
    63121 aaattgcaga ttggcaagac acgataccgc tgagaactgc tccggcagca tgtaattgag
    63181 catcaccaca tcagcttgtt tgaggatctg catctcgttc acttctgcgc gtgaataatc
    63241 cagcagaatg gtttgcttcc ccgccgccgc tttgtattta gccagattaa tcgccggctt
    63301 agccataaac gaatcatcct gcggcaaaac gccatcgggc tgcgtttctg gcatccatag
    63361 ctctttgagg aacatttcgg cgcgatggat aaacgcatcg tcgctacagc cgaactggcg
    63421 ggcaatattc agcgcctgtt gaacgttgta gcgggccata tagctggtga aggcgttatt
    63481 attgacatgt tcggtatatt cgtctggccc aataacatca tgaatttcca gacggtcgtt
    63541 aaccctcacc gcgcggctaa tccagaactt tgcagtttcc agaagtagcg ccatgccttc
    63601 atgagcaatg aaactttcat cccccgtggt ctgccagtat tgaataaccg cccaggcgat
    63661 atcggccacc agatgatgtt ccgcctgcgc cgaggccact ttttgccgca gcccggtacg
    63721 aatgttaatg gcggcaaatt ctggcgtctc ttcttcgccg ctgcgcgcgc tttcccacgg
    63781 aaatagcgcg ccctgccagc cgctgcgccg tgctttctcc tgcgcgcctg gcaagttgtg
    63841 ccagcgataa cgcagtaaac ttcgggcaac cgtcggatcg ctaaacagat ggaacggcag
    63901 caaaaatact tctgtatccc agaaaacgtg gcctttgtag ccttcgccgg ttaagccttt
    63961 tgccgcaata ctgctgcgct cgtcgtgagc cggcgtcatg atgcgcagat gataaagcgc
    64021 ataatctaac gcttgctgat cgtgcgcatc gccgccattt accgtgatac gacgtttctg
    64081 ccaccattga cgccagtttt ctgttgatgc tgcaagaagt tggtcgtaac tctgctgcgc
    64141 gcatatttca agctggcgaa gcgacgcgct gccccactcg tctaaaacgg cttgcctgtc
    64201 atcccgccag tcgatccaca ccagtttttg caacgtcact gtctcgcctg catgaagctg
    64261 cgcactggta tgttgcagca aacggcgctc tttggcggta tagcattgct gcacatcacc
    64321 gctcacctta cagcaacagc tgatggccac atcactgcgt ccatcctggg tggtgtagat
    64381 cccctgcatc agatgctgac caaacacccg cacctgggtt tcgtcgagat gttggcgacc
    64441 gtggttggtt tgcgtggcgt cgatacctgt tgaaatcagc actgacgcgt cggcgtccag
    64501 tggcgtaata gtgatttcca gcgcaatgag cggcagttgg tctgccgaaa caaagcgacg
    64561 gctggcgatg gtgtaacctg cgccgttgct ggtacgccag acaacgttgc ggcgtaattc
    64621 gccactggca aagtcaagct cacgctgcca ggcttcgtgg gataacgaga aaacctcacc
    64681 atttatggca atctccatcc ccaagatatc aggcaggttc accagttcgt tgatttcacc
    64741 ttttcccgcc cgatgataca gccccgccag atacatccct cgcgtctggc gcgtgtaatc
    64801 ttcttcatgg ctggcgcgaa gcccaagata gccgttcccc tgcgccatca gcgatgcata
    64861 cttgttcagg gtatgctggc tgaaatgggg ttctgataac gttactggcc tggtcataat
    64921 tccacacgcg tccctgtttc tgcggattga tataacgccg ccaccagttg ctggatgatg
    64981 tacccctgct cggcgtcggc tatcatcacg ggcttgccct gtacgtgatt gataaaggct
    65041 tccatgctgc ccaaatggcg gttgtcgtct gccatttccc gttgcatcag cgtcattaat
    65101 tcaccgttgt tatcggtgta gatatgtgct ggaaacagcg tcgcaccggc tttatcacca
    65161 caaaagctga cgttcataat cgactgttcg cggatgttga gcgcaaatga cgtttccagc
    65221 cacagaatgc cgccgttatg aaattcaatg gtgccaaaca gcgaatcttc gacgctgtaa
    65281 gttgccggat cccactcgcc aaattgacca cagctctttt gcgtgccgat cttttgaaag
    65341 ctatgcgcat tcacgctttt caccgcagga tagcccagca catacatcgc agcatccagc
    65401 atatgaatgc cgatgtcgat cagcgggcca ccaccctgca gttctttatt ggtaaagaca
    65461 ccccagccgg gaacgccgca gcgacgcagg gcgcgggcgg tggtaacgta aatctctccc
    65521 aaaacgccgt tggtcacctg ttcacgcagc tgttgcgtat cgagcgcaaa acgatggtga
    65581 aagtcgtagg ccagcacctt gccctgtttg cgcgcggtat cgcacatttc ccgcgcctgt
    65641 tctggcgtca tggcgggcgg tttttcgcac atcacatggc agcccgcttc cagcgccatc
    65701 agggtatgtt cgtaatgaaa acggttaggt gagcagacgc taaccacatc aggtttcacc
    65761 gccagcagca tggcctgcgg atcgtcccac acggatgcat tcccgtattt ttctgccagc
    65821 gcctgcgcct gggaaaggcg gctgtcacag acagccacca gttccagatc gttgcgggtg
    65881 cagtagtacg aagcatgaac cttatccgcc acctggcctg cgcctattat cgcgacccgc
    65941 agtggagagc ttgtcattgc acttttcaca aagacctctt agcaggtacg caaccaggtc
    66001 aacgaatcac ggtacgcctg ggcaggatct tctgcccgga tacgcccttc atacaccaca
    66061 taaccctgat agttatcggc gcgcagctgt tcaaacagcg cgtggaaatc gagggtgccg
    66121 ctgcccggct ggtaacgatg gttatcggca atatgcacat gaccgagcaa gtcgcggttg
    66181 tcatgcagcg cctgcgccag gttatcttct tcgatgttca tgtgatagaa atcgccgata
    66241 atctgcacat gtttaagatt gttttcgacg ctgtaacgac gagcatcggc gagggtgttg
    66301 atcatatgat cctgatagcg gtttaacggt tcgagataca ccacggttcc ggtacgcgcg
    66361 gcgacctgtt ccagtacgcg cagggaatca ctcaccattt tgcggtcgcc gtccaggcta
    66421 cgcggcgagg tcatcggcgg taagcggaag gtaaacatgc cccacgctgc cggaacgacg
    66481 atacctttac cccccacttc tgccagcgct tcgagaatgc gttcgatctg ctttaagcca
    66541 ttaagacgac gctcttcgat aaagtcacca atccagccgt catagccgcc gcaggcggtg
    66601 gtcaccggta aaccggtttc tttgatcgcc gatttgactt cttcgatgtt gttgaccagc
    66661 agtttgccgt caatctcaaa accatcgaag cccatctctt tgatataacg aaatttctcc
    66721 agaatatttt ccgggaagaa cgcctgattc tgtgtgccta ttttcatttt ttcattcctt
    66781 tgaattaaaa cgtgacgccc attttgatgc tctgttccgg atgctgatcg acatactgca
    66841 tgtagctttc cgggctgttg gcaaaggtca ctaccgggtc gattaaatct tcgcaattga
    66901 gataaccgtt catcagcagt ttccagcagg tttcttcaat acgcttacgg ctccagcgcg
    66961 gataatccgg gttcggttca ctgcacgcgc gagagaagac aattttggcg ttattgaaat
    67021 gcgcttcgcg tccgaggtta aaaccttcag caaacggttt agcaaacgcg acataggaga
    67081 tggtgccacc gtaagccagg ccgcgcagcg ccgactgcag cgcgtcggcg taaccgctgg
    67141 tttcgataat cacatcggca ccctgcttgc cggtcagcgt tttgatctct ttgcctacgt
    67201 cagtgccaat gggattaagg cagaaatccg caccgtgacg acgagcaata tcacaacgat
    67261 gggcaatagg atcgacgcca attaccaccg acgcgccagc gcgtttagcc agttggatgg
    67321 caatttgacc gatcgcgcca agccctacca ccactacaaa atcccctacg cgtacgttgg
    67381 catcgcgaac gccactcatg gcaaactgcg ccgggtcgta gcagacagcg tttttccagg
    67441 agctgccttc cggcattttg cgcagcttgt agttattcac tgcgttaatg atgatcgtct
    67501 cggagagcgg gccgtagccg catacgctgt cgcccaccgc gtagtcggta acgtcgctgc
    67561 cgcactcgat aatgtcgcca accaccatgt tgccaagctg gaatttgcca aactcaatgc
    67621 cgcgcggcgc atctgccgga cgcggggtga acatctgcca ttcgccgtta aagtcttcat
    67681 cgataaacgg gctggcggcg cggaagtcga ccacttccgt tccgtgtttc ggtgcaccga
    67741 aacgcacgcg gatcttcact tcattggcta aaatcgcccg atcttcatac tcaaccagcg
    67801 cagcaacgcg cggtgctgtg gctactaact ttttcatgta aaactccttg taaagaaact
    67861 ggccgcttta gcccttcacg ccaccggcgg tcaaaccact cttaataaaa cgttccgaaa
    67921 gcgcatacat aatcaccacc ggcaatgcgg tcaccagcga ggccgccatc atccgtcccc
    67981 agatgtaatc tggcgtactg aacagcgcgt tcaggccgac tggtaaggtg aaattgctgg
    68041 cgctggagag gaaaatcgac gcaaacagat agtcgttcca tgccaccata aagcagtaga
    68101 cgaatacgga tatcagcccg gacatcgcca ggggcacggt gatacggaag ataatttgca
    68161 gacggttgag gccatccatc atcgccgctt cttcgatctc atcagggatg gtgtcgaagt
    68221 aacttttcag cataaacacg gcggtgggca gagtttgcgt caccatggta atgatcagcg
    68281 ccatctcggt gtcgtaaatg ccaagtgcgg tgataatttt gaacagcggc accaccagca
    68341 aaatgccgga gaacatatac accgtgtaaa agctggcgtt gatggtcatc cggcctttaa
    68401 agcgcaggcg agaaagcgcg taagctccga gaatgccgag aaataccgcc accacggatg
    68461 aaaccaccga caccaccatg ctgttacgga agtagtcgac aaacgggaaa atcaccgggt
    68521 taaaaatgtc gacataatgc tccagcgtcc actgttgcgg cagcagcgta ggatgcagcg
    68581 agatcgcctc ttttgcgctc ttgaacgagg tcatcagcat cacaaaaaac ggaaacagcg
    68641 tgatgatgag aaacagcgcc agcccgcggt aaaaaccgat acgactgagt gtgcgtttat
    68701 ttgttgccat tgaggttcac ccgtttcctg gtcagcagaa tgacagcgaa aataatgatg
    68761 aagagcacca ccgagatcgc cgccgctttc cctaagtcat taaaggcaaa ggcggttttg
    68821 tagagataga caccaagaat atcgaccttg gttgtcagca gataaacatc cgcgaacatg
    68881 tagaacatcc agatggtgcg aagggtcacc accgtcgcca ggaccggcat aatcgcgggc
    68941 agcgtgacga tacgaaaccg ttgccaggca ttagcgccat ccatctctgc cgcttcgtac
    69001 agcgatttgt cgatggtctg caaaatcgcc agaaacgaga taaaggcata cgggaagtag
    69061 cgccagatgg cgaagatcac caccagcaca aaactactgc ccggattatc gaaccacagc
    69121 ggtgcctgct cataaagatg cagaagatcg acgccgaggt agttcacaat gccgtagccg
    69181 ttgttgaaca tgtatttcca ggcgaacacc agcgaaatag acggcgttac gtaggagagg
    69241 atcaccagcg aacgcgcggt tttgcgcagg cggaactcgc ggttgaaaaa catcgccact
    69301 gccagcccga gcacggtgct gcccgccacc accaacgcgg tataccagac cgtcatccac
    69361 agcgagtgcc agaagccggg atcggagaga atacgcacat agttgcttac cccaacaaac
    69421 gttgactcga tgttgggatt gagcggcaga cgtaaaaaac taatttcgat attcgacacc
    69481 attggccacg caaccagacc gcccagcagt aataagctgg gtgcgagaag caacagcgca
    69541 aagggcatat cggaacgacc tgaaaaaagc ctgttcattg cggattcctg tcctgggttt
    69601 agtgctgttc gatcagttca tccagctttt tctggctcgt ttgcagcgtg ccagggagat
    69661 cggctttacc tacggtgacg ttatgcacca tggagctcac caccccagac cccgtcacat
    69721 cgcccatgcg ggtaaatttt ttatccccta ctgcgccaaa gacctgaata ttcggcagtt
    69781 caccgattaa ctgatttggc agttcaccca gcgccttaat aacgtcgttg tctttccagg
    69841 tggaagtagt gacaaccgct ttgttcaccg gcagcgccgc gcctggcgac atcatcaccc
    69901 agtcggcaat gttgtctgcc tgctccataa aggtgacaaa tttttcagcc gcttccgttt
    69961 cttcagtctt ttgtccggcg gtaatggtca gcgaggtcaa catgccgtag acggcagagt
    70021 ttttctcggt tggcaccacg aaaccgacgt ttttcggatc gccttcttta atcacagccg
    70081 gaaggatata ggtggagtaa atcgccatcg gcgcggtgcc gttcataaag gcgtctttca
    70141 cttccatgat gtcgttagaa cccggcatgg tgttggcagc aaggtcgcgg taataggtca
    70201 gcgcctgcat catctctggt gaatcaaggg tgattttgcc ttcggcgtta aagacgttgg
    70261 cctagttgga taacgcaaac tgggagaagg attgttccgt cagcacgctt tctgccgtag
    70321 gcagcgcaat accgtatttt ttattcgccg ggtcattcag tttctgtgca acgtccagca
    70381 gctgttgcca gtttttcggc tcctccagcc ccgcttttgc cagcacatct ttgcgatacc
    70441 agatgccgcc aatccaggcg ctgacaggaa caccggtcca tgcgctacca tcttcggtac
    70501 gcacaatacg cagtacgcca tcgtaaaacg cgccttcacc aacgttgctg atgaccgtgg
    70561 caaccgcctt gcgatcgata agctgctctt tgtccataac tttggcgtag tcatgactgg
    70621 tttcaatcac ttccggcagc gagccgctac gtgaaagagt gatgactttg gtgttataag
    70681 cgtcctcttc gacgggcact tgtttaacgg tgatacctgg gttttctttt tcaaaacggg
    70741 caatcaattt gctgataact gcctgacgct cctgctccac cgaagaatgc ataaattcga
    70801 tggatacatg gggtttattt tcttctttac agcctgaaat cagggcgcag gaaaccagtg
    70861 ctgataacag cacgattttt gatttaatca tgagatgttc ctttttattt aatccacata
    70921 acctgccagg ggcgcagcgt tattttatta cccaatatat caacttcact aatcagatcc
    70981 cgaccgtgca ggccagtaat atttacatgc tgaatatttt tactgacatt aaacaatccg
    71041 gtcagacaat taccctctgc gttacttctt tgaatacgca ttacggatga attaaccgta
    71101 tcgatggtaa aattattatc cggatgaaac tcgttatggc tgcgacgaag tgtaattaaa
    71161 cgcgacaact catgatatac cgcatgcctt aatgtagctt catcgttcag ttctcgggtt
    71221 atctcttcac tgtgatattt tttacggtta atcgcacggt tatatccgag tttttcgaca
    71281 cctgcgtaat cattacgcga gcccagaata ctttgaatat atatcgctgg cacaccgggg
    71341 aaacttaaca aaatcgcatg ggcaaggata aacctggcgc aacgttcttc atcgctactc
    71401 tcgcggcggc ttaacgcatc catataggtg acatttattt catagggact gcgcgtaccg
    71461 tcgggattat ttttccagtt tactaatgca ccttcctgct gtaatgcctc gaccagcgct
    71521 aatatttcgc tttctggtaa caagccccgc agtgggttca gcccgatgcc atcgtgagag
    71581 gcgaggaagt taaaccaggt cgttttaccg gagggcaagc tcagactttg cgcccactgg
    71641 cacagcgcct cgacgttctg tttttgcacc gcatgtaaca ccagcggcgg cagcgagaac
    71701 tggtacacca tatgcgcttc gtcatcgcct tcgccaaagt aagcaatgtt atctttatgc
    71761 ggaacattcg tctcagtaat aatcaccgta cctggcgcga tgtcatcaat aatcgaccgt
    71821 aacagtttga taatcagatg tgttttttcc agatggatgc agcttgttcc cggctctttc
    71881 cacataaagc caacggcatc cagacggaca tattctgcgc ccttttccag gtaacaaagc
    71941 aaaacatcca ccatcgccag caacacgtca gggctacggt aattcaggtc aatttgatcg
    72001 tcgctaaagg tggtccataa atggcgcgtg gaattatcat ccatttgaaa cggcgttaat
    72061 agcggtaatg cacgcggacg ggtgacagcg ctgagatcgg tttgcggatc aaccgcaata
    72121 aaaaaatctt caaaacctgg ctgctgttgt aaatagtttt taaaccattc actttttgcc
    72181 gacatatggt tgcagacaaa atcaaacatt aaatgactgc attcaccgag ctgctgaata
    72241 tcctgccact acccgcttca ctggcgacct gatgataatc aattaccgaa aatccatcat
    72301 cagatgacca tggataaaaa ggtaataaat gaacatgtga aaaaatgctt tgcagccatt
    72361 gatggtaaaa ctgattaaat gtgggtagtg gttttaaatc attactgtga aattgatcgg
    72421 cataggtaat taacacgaca tcactttcat cccagtgttt tttacgtcgc tgtgtaatta
    72481 atcgtttcgc actctcaaga cgcgttacaa gtttctgtaa atgagttgcg gtaaatgttc
    72541 caccgtagat ttcgtccagg taatccgtaa ttttctgttt catgttgcct ttcctggtag
    72601 cggtcccatg aaatgagaga ctactccgca gaaaataaac tgtcaattga gcacaagggt
    72661 tttagtcacg caggaaatca ttttttacca gggaaaaagc gtatttgtga gctgacgcaa
    72721 ataatctata gggatgtaaa taaggtgtga cagaaaaaaa cggcgcataa gcgccgctca
    72781 tggtgaattc ttttaacctt tgaccttcgg cattgcgatg tctttcaggc caccggcgtt
    72841 ctcaacgtgg gtatatccca tctcgctaag gatctctttt gcctgccctg attggcgtcc
    72901 ggcattgcaa tacactttca cggtgtcgtt tttatccgga acgacggtgg caatgcgctc
    72961 tttcacttcc ttcaggggaa tattgatggc cccctgaacg tgctcttgct gatactgctc
    73021 tggaacacga acatcgatcc agtgttcagc ggcgaaaacg ggcagtgaaa acaccagtgc
    73081 cagagcaagt aagccttttt taaacatgta agcgtccttc tatggatgaa tattgctatc
    73141 cattatacgc ctgagattta acaatttttg cgaaactccc ctcacctttt ataacgctgc
    73201 gccagtttat tggcagcccg acttaacagc ggttccagcg ccacagccag caacattttc
    73261 agcggtcggc gagcaactga ttttatcgcc cagcccgcca cacccgctgg gccatagcgc
    73321 agtgcggtaa gaagtaccag cttgcctgct aatttgaaac caggctttac cttttgcccg
    73381 gcctgttgcc agcgagtatt cataactttc ctcacagttg acggaaacgg ctacgtaacg
    73441 tgaatgtatc ggaagtgaca taacgttcca tctcgcgtaa acgcgtttca cttgccgcca
    73501 gttcacggtc gacttcatcc agcaattcac tgctggaagg tagctgctca ccaaaggcca
    73561 tgttgtccgg cattggatca agcgcaaatg acaaaatgat gtaagcaacc agggtaaaca
    73621 gcgccagacc gaagaaaatc gacaacacca ccaggatacg caccagtttt accggtacat
    73681 caaaatagtt ggcaatcccg gcgcagacgc cacggaccat gccctgctgt ggaatacgcc
    73741 ataatttttt attgagatta atgcccgcca ttagcgatcc ctccagttcg gatgttctgc
    73801 atcaagaata gattccagcg cctgaatacg ttcgcgcatc cgttttgctt catcagccag
    73861 ttgcgctaat cgctgctgct cactttgcga caattcactg cgaccagaac gattgctgta
    73921 atgcagccat aaccagatcg gtaaaacaaa cagcacaaaa atggttaatg gaatagccag
    73981 aaatagcgcg ctcataagta ctccttagac gatgagcggc ggcgcgttcg gacgccgcct
    74041 ggatgttatt attgattgtc ttgcttcatt ttggctttta attgtgccag ttgctcgctg
    74101 attgcatcat cggctttcag ttcggcaaac tgatcgtcca gcgatttttg tttaccgaag
    74161 ctgtggcttt ctgcttccgc ttccatctgg tcaatacgac gttcgaaaga ttcgaaacga
    74221 gccattgctt catccagttt gccactgtcc agctgacgac gcacatcgcg cgacgagttt
    74281 gccgcctgat ggcgtaacat caatgcctgc tggcgagcgc gtgtttcgct caatttgttt
    74341 tccagctcgc caatctcttt cttcatgcgt gccagcgtat cgtccaccag cgtcacttca
    74401 tgttccaggg acttaatcag atcggtcagt ttctgttttt caattaacgc cgcacgcgcc
    74461 aaatcctctc tctctttcag cagcgccagt tcggcttttt cctgccattc aacttcacgc
    74521 gccgacgctt gttcaatacg gcgagtcagc tgtttctttt ctgccagcgc acgcgccgaa
    74581 gtagaacgta cttcaaccaa tgtatcttcc atctcctgga tcatcagacg caccagtttc
    74641 tgtggatctt ccgctttctc taacagagcg ttgatgttgg cattcacgat gtcggcaaag
    74701 cgagaaaaaa tacccataat gttgtcctct tgatttctgc attcaggatt gtcctgctga
    74761 actgttaata caatttgcgt gccaattttt tatctttttg atttataaag atctgattga
    74821 agaatcaaca gcaacatgcc aggatgaatt agctaattac actaacaagt ggcgaatttc
    74881 atcatggcag aatacaaaga taatttactc ggtgaggcga acagctttct cgaagtgctg
    74941 gaacaggttt cgcatctcgc accgctggac aaaccggtgc tcatcatcgg cgaacgcggc
    75001 accggtaaag agctgatcgc cagccgcctg cattatctct cctcccgttg gcaagggccg
    75061 tttatttccc ttaactgcgc ggcgttaaat gaaaatctgc tggattccga actgtttggt
    75121 cacgaagcgg gggcgtttac cggtgcgcaa aaacgtcatc cagggagatt tgaacgtgcc
    75181 gatggcggta cgctatttct tgatgaactc gctacggcac cgatgatggt gcaggagaaa
    75241 ttattgcgcg tgattgagta cggtgaactg gagcgcgttg gcggcagcca gccgttgcag
    75301 gtgaatgtgc ggttggtatg cgcgacgaat gccgatctcc cggcgatggt caatgaaggt
    75361 acttttcgcg ctgacctgct cgaccggctg gctttcgatg tagtgcaact gccgccacta
    75421 cgcgaacgtg aaagcgacat tatgttaatg gcggaacact ttgccatcca gatgtgtcgg
    75481 gaaatcaagc tgcctctgtt cccggggttt acggagcgcg ccagagaaac attgctgaat
    75541 tatcgttggc caggaaatat tcgtgaattg aaaaacgtgg tggaacgttc agtgtatcgc
    75601 cacggcacca gcgattatcc gcttgatgac atcattattg atccctttaa acggcgtccg
    75661 cctgaagacg ctatcgccgt ttcagaaacc acctcgcttc caacactgcc gctggattta
    75721 cgtgagtttc agatgcagca ggaaaaagag ttgctgcaac tcagtttgca acaagggaaa
    75781 tataaccaga agcgcgcagc tgaattgctg ggcttaacct atcatcagtt ccgcgcgttg
    75841 ttgaaaaagc accagattta gcgaagttgg gcaatacaac ttgccggatg cggcgtgaac
    75901 gccttatccg gcatacataa tcaatacgtc gtttgcaggt ctgataagcg cagcatatca
    75961 ggcattattg cgttgcccct gcttctgcgt attaatctct cagcgcatcc tgcaaaattt
    76021 tcattgccgc atcgaattgc gcatccggga tggtcagcgg atacaggaaa cgaatcacgt
    76081 tgccgtatgc gccacaggtc agtagaagca gtccctgcgc cagcgcgcgt tgctgaattt
    76141 tttgcgcaat ggcggctgac ggctcgcccg tttgcgggtc gttaaattct gccgcaatca
    76201 tcgagcccag accacgtacc gccgcgatgg ccggaacgtt ttctttggca tcaatcaggg
    76261 ttttcgtcag acgctgcccc aactgcatgg cgcgctcaca gagtgactct ttgtcgataa
    76321 tgttgagcac cgcgtgcgcg gcagccaccg ccagcggatt cccggcgtag gtgccgccaa
    76381 gcccgcccgg cgcgggcgcg tccataatat tcgcgttacc gaccacaccc gaaagcggca
    76441 tcccgcccgc gaggcttttc gccatcgtca ttaagtccgg cttatcggcg taatgatcca
    76501 tcgcaaacag tttgccggta cgcgcaaagc cgctttgcac ttcatcggca atcatcacaa
    76561 taccgtgctc gtcgcacagg cggcgaatgg cggcaaccag ctcttttggc gcaacgttga
    76621 aaccgccctc gccctgcacc ggttcgaaaa taatggccgc gacctgcttc gcttcgatgt
    76681 ctgatttaaa caagcgttcg atggcgtcga gggagtcctg tgttgaaatg ccgtgtaaat
    76741 ctgacggata aggtacgtga tacaccgaac cagggaacgg gccgaagccg attttgtacg
    76801 gcgcgacctt tccagttaac gccatagtca tatacgtacg accgtggaag ccgccgctaa
    76861 acgcaatcac gccagggcgt ccggtatggg cgcgggcgat tttcaccgcg ttttccaccg
    76921 cttccgcacc ggtggtgaag aacgcggttt tggcctgccc gctcaccggg gcaagggcgt
    76981 tgattttctc cgccagggtg acgtagcttt catatggcac aatctgatac gcggtgtggg
    77041 taaactgttg cagctgctgc tccaccgccg cgaccagatc agggtggcga tgtccggtat
    77101 tcagcaccgc aatgcctgcg gcgaaatcga tgtactcgtt gccctcaaca tccttcagcg
    77161 tggcgttttc agccgactgg gcgaagaagt tacacatcac gccaaccccg cgcggggtgg
    77221 cagaaagacg acgctgatgg aattcattgt tgctcatgtt tcacaccttt tgactcttca
    77281 ctgttaatcg gaaattaaac gcccagacga tcgcgcaggc tgtaatacgc cgcgcccatc
    77341 gcggtaaacg gtacgcgcag cgtgcgcccg ccggggaacg ggtaatgcgg cagattggcg
    77401 aaggcatcga aacgttcggc gtcgccgcgc agcagttcgg caatcaaacg tccggcgaga
    77461 tgagtacagg tgacgccgtg gccgctgtaa ccctgcatgt aatagatgtt ggtatcgagg
    77521 cgaccgaact gcggcatacg cgacagggtc agcaggaagt tgcccgtcca gcgatagtcg
    77581 attttcacgc ccttcaactg cgggaaggtt ttcagcagtt tcggcaccac aagacgctca
    77641 acgtcatccg ggtcgcgcgc gtcgtacacc acgccgccgc cgtacagcaa acggttgtcg
    77701 gcggtaagac ggtagtaatc aagcagataa ttacagtctt ccacgcagta gtttttcggg
    77761 atcagcgaac gggctaaatc atcggaaagc cgctcggtgg tgatcacctg cgtaccgcac
    77821 ggcatgctgc gtttcgccag ttccggctcc actttatcgc ccaggtacgc attcccggcg
    77881 acaatcacat acttcgccgt cacctgacct gtggctgtac gcactacggc gggcgtggtg
    77941 tgctggatct gcgtcactgc ggaaagctca tacacgcgcc cgccgttcag gcggatagcg
    78001 tccgcttcgc caatggcgag gttcagcggg tgaatatgcc cgccgctgtg atccagcagc
    78061 gcaccagtgt agcgatcgct ggcgacctcg cggcgaatgg cgttggcgtc cagcatctcc
    78121 agctgtttat tgccgtagcg ttcccagttc tctttctgct cttcaagtgt ggcgagctgt
    78181 ttatcattca tcgccacaaa cagaccgccg gggcggtagt cgcagtcaat ctgataacgt
    78241 ttgatacgtt cgcggatgat ctcaccacct tcgaacatca tgctgccgag catacgggcg
    78301 gtgtccatgc cgtagctttt ttcgataacg tcgatgtcgc ggctgtagga gttcacaagc
    78361 tgcccgccgt tgcgcccgct ggcgccaaag ccgatgcgtg aggcttccag aaccaccacg
    78421 tcaaagcccg cttccgccag atgcagtgcg gaagacagcc cggtatagcc gccgccaacc
    78481 acgcaaacgt cgcaggaaat tgactcattc agcgtgtcga atggcgcgta tttattcgca
    78541 ctggcggcgt agtaactgct ggtatgttct gtcatttcag gcctccaggc ttatccagat
    78601 ggttttcagt tcggtgaatt tttcaagggc atgcagggat ttgtcgcgac cgttgccgct
    78661 ctgcttatag ccgccaaacg gcacggtcat atcgccgtcg ttgtagttat tgacgaagac
    78721 ggaaccggct ttcaggcgtc ggctcatgcg gtgcgcgcgg gagaggtcgc gcgtccatac
    78781 cgccgcgcca aggccgtact ggctgtcgtt ggcaagctgt agcgcctgtt cttctgatgt
    78841 gaaacgcgtg accaccagca ccggaccgaa aatctcttcg cggcttaagg acgcattcgg
    78901 gtctacatcc acaatgatgg tcgggccgat ggcgacagcc agcccggcgt tacggccatc
    78961 caacaacagt tgccctttgc tttcgccttc ctgaataaag ctatggaccg agtcggcgtg
    79021 ggcgcagtcg attaaggtgc ccatggtggt tgcgggatca agtggatggc ccggctgcca
    79081 gttttgcgcc tgctgtttta acagggctaa gaattcatcg gctatgctct cttccagcaa
    79141 caggcgcgtt ccggcgatgc acacctgccc ctggttgtag aagatgcctg ctgcggtggc
    79201 gcttgccgcc tgttgcaaat ccgggcagtc agcgaaaacg atgttggcgc ttttgccgcc
    79261 cgcttccagc cagacgcgtt tcatgttgct gtcgcctgca tctttcagca gctgtttccc
    79321 ggtacgggtc gaaccggtaa aggcaatggc gtcgatatcg ttatgacgcg acagcgcctg
    79381 ccccgcttca tgaccaaaac ccgtcaccac gttcaacaca ccatccggca agcctgcttc
    79441 tttcgccagc cccgcgagac gaatcgcact gagcggtgat ttttcagacg gtttcaggac
    79501 aacgctgttc cctgctgcca gcgccgggcc aagcttccag caagtcagca gcagcgggaa
    79561 gttccacggc acgatggcgg caatcacgcc gaccggttca cgcacgatca tcgccagctc
    79621 atggctactg gtggtcgcca cttcgccata cactttgtcg atcgcttcgg cgtaccagcg
    79681 aatggcgcgc gccgcgccgg gtatatcatc acgcagactg tgacgaatcg gtttgccggt
    79741 gtcgagagtt tccagcagtg ccagctcttc ggcgttggct tctattaaat cggcgagttt
    79801 attcagcacc gctttacgtt ttgccggaga agagagtgac cagtcgccgc gttcaaatac
    79861 gccgcgtgct gcgctcaccg cacggtcgat atcgacgctc ttgccgcgcg caatattcgc
    79921 cagcggtgcc tgggtaaccg gatcaacggt ttcaaaggtt tcattttccg ccgcagcagt
    79981 atattcaccg ttaataaata agcggttttc aatggcgaga cttaacgctt tatcctgcca
    80041 gtaagccaga tgatgaaaat tcattatgac tcctgtttca cgtctatcag atatatgcag
    80101 tttgtgaacg tcagggcgca gagatccccg ggccgtatgc ccgtttattt ctggcgacat
    80161 tctttatgga ttgccgggcg cattgcgctt acccggctta cagaacaaaa agattaaaac
    80221 gtggtgggcg tatgggcgct gataattcgg caaatacctg ccgacgtatt actgaaactg
    80281 tgcgggatgc cggtattaat ggcataactt tgccccgcga cgaggtggta atcctgacca
    80341 ttaatcgtca gaataatttc accttccagt acagtgccta tttcctcacc ctgatgctta
    80401 attctttccc cagtggttgt gcccggctgg tacgtttcaa agatcatcgc cagcgtgcga
    80461 ttcgggttac cgttatgaac cagcttcatt gacacaccct gactgcccat ctcaattaaa
    80521 tcgtcctgat taatgacgac ctgcggctca tcaggttttt ccggctcgga aaagaattcc
    80581 gagagtgaca gaccatacac cttcagcagc ttttgcagcg tactgatggc agggctgact
    80641 ttatcttgtt ctatcgtact gatagcactg tgagtcagcc cggagagttc ggcggcacga
    80701 cgttgtgaaa gcccctgctg ctggcggatt tccgacaagc gttttcctgg cgccagtccc
    80761 tcatcactca tatttgcatt tccttaaact gtagtggtca gagtcgctgt ttttcagcga
    80821 tatggtgctg acaagcggtg ataaagccct cgaacaatat acgcgaaagc gcgtactcgc
    80881 tactgttcca ttccgggtgc cactgtacgc ccagcgcaaa aggatgattg atgacactaa
    80941 ccgcctcaac taagccatcc ggcgagcgag cttcaacacg caaccgtggg ctaacgacct
    81001 tcgccccttg tccatgcaga gagtttaccc aaaagttgct acattcaggt aacaatgcag
    81061 acagcaatcc cccctcttca acctgaactt catgcgacgg tgcatattgc tgttccaccg
    81121 gcagttcggg atcttcccga tgttccagca attcaggctg ttcgcacagc ttgcgatgca
    81181 acgaaccacc ggttgccacc accagttcct gtaaaccccg gcagatggcg aaaatgggga
    81241 tgcgcctttc gagtgcggca ttaattaacg ccatgctcag aagatcacgc tcgggatcgg
    81301 cgtcaggctc atcgccgttt tcaccatata ggtgcggctg cacattgctg ggactaccag
    81361 gaaggtaaat gccatcgagt ttcggcaaaa gttgttcaag taacgacggt tccgccagcg
    81421 catgtggcag cgcaattggc aagccgcctg cgtggatgat ggcattcagg tacttttctt
    81481 gcagagtctg ggtcgcatgc cccttaagcc tgttcctgca cattacgaca ccgataaccg
    81541 gattgttcat tatattttcc attctcaatc tcacaaagtg gactaaatta tcgccattac
    81601 tgcggtttaa atgaccttta tgttcaatat tttttcaatc tagcagtggg ttttcatttt
    81661 tgcaaactca atttaacatt tgacaaacat ttagtttgca tacagattcg aatggtggtc
    81721 attatatttt acgctttgat aacgagcgga aaacaaacca aaggcgaaga atcatggaaa
    81781 ccaatatcgt tgaagtagag aactttgttc agcagtcaga agagaggcgg ggtagcgcct
    81841 ttacgcagga agtgaaacgc tacctggagc gctacccgaa tacgcaatat gttgatgttt
    81901 tacttaccga tttgaatggt tgcttccgtg gaaaacgtat tcctgtttca agcctgaaga
    81961 agctcgagaa agggtgttat ttcccggcct cggtatttgc aatggatatt ctgggcaacg
    82021 tagtagaaga agcaggtctg ggtcaggaaa tgggcgagcc ggatcgtacc tgtgttcctg
    82081 ttctcggttc cttaacccct tctgccgccg atccagagtt tatcggtcag atgctcctga
    82141 ccatggtcga tgaagatggc gctccctttg acgttgagcc gcggaacgtt cttaaccgcc
    82201 tgtggcagca gctgcgccag cgcggattgt tcccggtcgt agcggtagag ctggagttct
    82261 atttactgga tcgccagcgc gacgctgaag ggtatctgca accgccctgc gcgcccggca
    82321 ctgatgaccg caatacacaa agccaggttt actccgttga taacctcaac cacttcgccg
    82381 acgtgctcaa tgatattgat gaactggcgc agttacagct gattccggca gatggcgcgg
    82441 tcgctgaggc ctcgccgggt cagtttgaaa tcaaccttta ccatactgat aacgtgctgg
    82501 aagcctgcga tgatgcgctg gcactaaaac gcctggtgcg tctgatggca gaaaagcata
    82561 agatgcacgc cacttttatg gcgaagccgt atgaagagca cgcgggcagc ggaatgcata
    82621 tccatatcag tatgcaaaat aatcgtggcg agaacgtgct ttctgacaca gaaggcgaag
    82681 attcgccgct gctgaaaaag atgctcgccg ggatgattga cctgatgccg tcatcaatgg
    82741 cgttgctggc accaaacgtg aactcgtatc gccgcttcca gccggggatg tatgtaccga
    82801 cgcaggcgtc gtggggccat aataaccgta ccgtcgccct gcgtattcca tgcggcgatc
    82861 gtcataatca ccgcgtggaa tatcgcgtgg cgggtgccga tgccaaccca tatctggtga
    82921 tggcagcgat ttttgccggt attttgcatg gccttgataa cgagctgccg ttgcaggaag
    82981 aagtcgaagg taacgggctg gaacaggaag gcttaccctt cccgattcgc cagagcgatg
    83041 ccctgggtga gtttatcgag aatgatcacc tgcgccgcta tttaggcgaa cggttttgcc
    83101 atgtgtatca cgcctgtaaa aatgatgagc tgttgcagtt tgagcgcctc atcacagaaa
    83161 cagaaattga gtggatgttg aaaaacgcat agtacccctt gcggggttgt ttcatcggcc
    83221 tgagcggagc caggcctgac atttgctggt aatccagaca tttcccggat gtcgcgttat
    83281 ggagcgcagg cagcaacggg cttccgttaa cgattaagac tgtgacgagg aaatgagatg
    83341 atgccgatga ttaaatcccc gcatggcgcg ggaggctgcg tatgcgcccc tcctgcgact
    83401 gactggactc cgcccccttt gcttcccttg ctaaacaggt ttgatttcag gtcgacacga
    83461 ccgcaaacct tattacgcag gggaggcagc aattatggct attaattcac cactgaatat
    83521 tgctgcgcaa cccggcaaaa cccgtctgcg aaaatcactg aaattgtggc aggttgtgat
    83581 gatgggtctg gcctatctca cgccgatgac tgtgttcgat acctttggca ttgtatctgg
    83641 cattagcgac ggtcacgtcc ctgcgtccta tttgctggcg ctggcgggcg tgctgtttac
    83701 cgctatcagc tacggcaaac tggttcgcca gtttccggag gccggttcgg cctataccta
    83761 cgcgcaaaag tcgattaacc cgcacgtcgg atttatggtc ggctggtcat cactgctgga
    83821 ttatctcttt ttgccgatga tcaacgtttt gttggcgaaa atctatctct ccgccctctt
    83881 cccggaagtg ccgccgtggg tgtgggtggt aaccttcgtc gccattttaa ccgccgcgaa
    83941 tctgaagagc gtcaacctgg tcgctaactt caataccctg tttgtactgg tgcaaatctc
    84001 catcatggtg gtgtttatct tcctggtggt tcagggactg cataaaggag aaggcgttgg
    84061 caccgtctgg tcacttcagc cgtttatcag cgagaacgcg cacctgatcc cgattattac
    84121 cggggcgacg attgtctgtt tctcgttcct cggtttcgat gcggtgacca cactttcgga
    84181 agagacacca gacgccgcac gcgtgatccc gaaagccatc ttcctgacgg cggtctatgg
    84241 tggcgttatc tttatcgcgg cgtcgttctt tatgcagctg ttcttccccg atatcagccg
    84301 ctttaaagac ccggacgccg cactgcctga aattgcactc tacgtcggcg gtaagctgtt
    84361 ccagtcgatt ttcctctgca ccacgtttgt gaacacgtta gcgtctggcc tggcctcaca
    84421 tgccagcgtg tcacgtctgc tgtatgtgat ggggcgcgac aatgtgtttc cggagcgcgt
    84481 gtttggctat gtgcacccaa aatggcggac tccggcactg aacgtcatta tggtcggaat
    84541 tgtcgcgatg tcggcattgt tctttgattt agtcaccgcg acagcattga ttaacttcgg
    84601 tgctctggtg gcgtttacct tcgtgaatct gtcggtgttt aatcatttct ggcgtaaagg
    84661 aatgaataaa agctggaagg atcacttcca ctatttgctg atgccgctgg ttggcgcgct
    84721 gacggtgggt gtgctgtggg ttaacctcga gtcaacgtca ctgacactcg gtctggtatg
    84781 ggcttcgtta ggcggcgcat atttgtggta tttgatccgc cgctatcgca aagtgccgct
    84841 gtacgaaggt gacagaacgc cagtgagcga aacgtaatag tcggatgcgc gccgcattat
    84901 cctgcctgag ggtaatgcgc cgcgccatta actataaaaa gatgtatgac ccggatcacg
    84961 agtgctatca tccgccctta tgcttagcgc attaacccca ggggcaaata atgaaccacg
    85021 atatcccgct aaaatatttt gatattgccg atgagtacgc gaccgaatgc gcagagcctg
    85081 tcgcagacgc tgaacgcaca ccgctggccc actacttcca gttactgctc acccgtttga
    85141 tgaacaatga agagatcagc gaagaagccc agcatgaaat ggctgctgaa gcggggatta
    85201 atcccgtacg cattgatgag atcgcagagt ttttgaatca atggggtaat gagtaattat
    85261 ttaattgcag acgatcggtc agtatcgttg tccggataaa gcgttcacgc cgcatccggc
    85321 taacaatgac acgcactgca gcgtgcttta atgggtttct cacgatcgta ccgctctgct
    85381 cctcgctaag ttgttctgtg agttatagtt atagagaaat cacgggtacg aatgttgaac
    85441 agatggcgac gccatctgtt caacgaccaa ccacacggcc ctcattacga cccctgaaca
    85501 caaacggtaa tggaaaatgt cattaaatat tagtaagata gcctctttac ataaaatccc
    85561 ttaatattat gcgccaggta ttatcgtctc ttttggtgat tgctggactt gtgagtggtc
    85621 aggcaatcgc cgcgcctgaa tcccccccgc atgctgatat ccgcgacagc ggttttgtct
    85681 attgcgtcag cgggcaagtc aacaccttta acccatccaa agcgagcagt gggttaattg
    85741 tcgataccct tgccgcccag ttttatgatc gactgctgga tgtcgatccc tatacctatc
    85801 gcctgatgcc agaacttgcc gaaagctggg aagtactcga caacggcgcg acctatcgct
    85861 tccacctgcg tcgcgatgtc ccgtttcaaa aaaccgcctg gtttactccc actcgcaaaa
    85921 tgaatgccga cgatgtggtg tttaccttcc agcgaatttt tgaccgcaac aacccgtggc
    85981 ataacgtcaa cggcagcaac ttcccctact tcgacagcct gcaatttgcc gataacgtga
    86041 aaagcgtccg taaactggat aatcataccg ttgagttccg tctggctcag ccggatgctt
    86101 cttttttgtg gcacctcgca acccattatg cttcggtcat gtcggcagaa tatgcccgga
    86161 agttagagaa agaagatcgc caggagcaac tcgaccgtca accggtcggc actggaccgt
    86221 atcagttgtc ggaataccgc gccgggcaat ttattcgcct acaacgtcat gatgacttct
    86281 ggcgcggtaa accgttaatg ccgcaggtgg tggtggattt aggctccggc ggcaccggac
    86341 gtctgtcgaa actcctgacc ggggaatgcg acgttctggc ctggcctgct gccagccagc
    86401 tatccatttt gcgtgacgac ccgcgcttgc gtttaacgct gcgtcctggg atgaacgtcg
    86461 cctatctggc atttaacacc gccaaaccgc cgctaaataa tcccgctgtc cgccatgcgc
    86521 tggcactggc gattaataac cagcgcctga tgcaatccat ctattatggt acggctgaaa
    86581 cggcggcctc tattttaccg cgcgcctcgt gggcctatga caacgaggct aaaattactg
    86641 aatacaatcc ggcgaaatcg cgcgaacagt tgaagttgtt ggggctggaa aatttaacgc
    86701 tgaaactgtg ggtgcccaca cgttcgcagg cgtggaaccc cagtccactg aaaactgccg
    86761 aactgattca ggcggatatg gcgcaggttg gcgtaaaagt ggtgattgtg ccggtagaag
    86821 gtcgctttca ggaggcgcgg ttgatggata tgagccatga tctgacgtta tccggttggg
    86881 cgacggacag taacgacccg gacagtttct tccgtccgtt actgagctgc gcggcaattc
    86941 attcacagac caacctcgcc cactggtgcg atccgaaatt cgacagcgtg ttgcgtaaga
    87001 cgctctcctc gcagcagctg gcggcgcgta ttgaagccta tgacgaagcg cagagtattc
    87061 tggcgcagga attgcccatt ttgccgctgg cgtcgtcatt gcgtttgcag gcctatcggt
    87121 acgatatcaa aggtctggta cttagcccgt ttggtaacgc ctcctttgct ggggtgtatc
    87181 gcgagaaaca ggatgaggtg aaaaaaccat gattatcttc accttacgcc gcattttgtt
    87241 attgattgtc accttgttcc tgctgacctt cgttggcttc agcttgagct atttcactcc
    87301 acacgcgccg ttacaaggcg cgtcactgtg gaatgcctgg gtgttctggt ttaacggcct
    87361 gatccactgg gattttggtg tttccagcat taacggtcaa ccaattgccg aacagttaaa
    87421 agaggtcttc ccggcgacga tggaactgtg catcctcgcc ttcggttttg cgctgattgt
    87481 tggcatcccc gtggggatga ttgccgggat tacgcgccat aagtggcagg ataacttgat
    87541 caacgccatc gccttactgg gcttttcaat cccggtgttc tggctggcgc ttctgttgac
    87601 actgttttgt tcactcacgc taggttggtt gcccgtttca gggcgtttcg atctgctcta
    87661 cgaagtgaaa ccgattaccg gttttgcgtt gattgatgcc tggctttcgg actcaccatg
    87721 gcgggatgaa atgatcatga gcgcaatccg ccatatgata ttgcccgtga tcaccctgtc
    87781 ggttgcgcca acaactgaag tgatccgcct gatgcgtatc agcactatcg aagtgtatga
    87841 ccagaactat gtaaaagcgg cggcgacccg cggtttgtcg cgctttacta ttttgcgtcg
    87901 ccacgttctg cataacgcgt taccgccggt tattcctcgc ctgggcttac agttttccac
    87961 catgctgacg ctggcgatga tcaccgaaat ggtttttagc tggccgggtt tagggcgctg
    88021 gttaattaac gccatccgcc agcaggatta cgcagccatt tccgccggag tgatggtgtg
    88081 tggctcactg gtcattattg ttaacgtgat ttctgatatt ttgggtgcca tggctaaccc
    88141 tctgaaacat aaggaatggt atgccttacg atagcgtata cagcgaaaag cgcccgccgg
    88201 gcacgctgcg taccgcctgg cgcaaatttt atagtgacgc ctctgcgatg gtcggcctgt
    88261 acggctgcgc ggggctggct gtactgtgta tgtttggcgg ctggtttgcg ccttacggca
    88321 tcgaccagca atttctcggt taccaattat tgccgccatc atggtcgcgc tatggcgaag
    88381 tttctttctt cctggggact gacgacctcg gacgcgatgt tttaagccgt ttactgagcg
    88441 gagctgcgcc aaccgtgggt ggcgcatttg tggtgacact tgccgcgacg atctgtggcc
    88501 tggtactggg caccttcgcc ggggcgacgc acggcctccg ctcagcggtg cttaaccata
    88561 ttctggatac cttactggcg atcccttccc tgctgttggc aattatcgtt gtagcgtttg
    88621 ccggaccgag tttgtcccac gctatgtttg ctgtctggct ggcgctgctg ccgcgtatgg
    88681 tacgttcgat ttacagcatg gtgcatgacg aactggaaaa agagtacgtt atcgccgccc
    88741 gtctggatgg cgcatcaacg ctgaatattc tctggtttgc tgtgatgcct aacatcaccg
    88801 ccgggctggt caccgagatc acccgcgcac tgtcgatggc aattctcgat atcgccgcgc
    88861 tgggctttct cgatcttggc gcacaactcc cctcgcctga atggggagcg atgctcggtg
    88921 atgcgctgga actgatttat gtcgccccgt ggactgtcat gctgccaggt gcggcaatta
    88981 tgattagcgt gctgttggtt aacctccttg gcgacggtgt ccgtcgtgca attattgcgg
    89041 gggtggaata atgccattac tcgatattcg taacctgact attgaattta aaaccggtga
    89101 tgagtgggtt aaagccgttg accgggtaag tatgacctta accgaaggtg aaatccgcgg
    89161 tcttgttggt gaatccggtt ccggcaaaag tttgattgcg aaagcgattt gtggggtgaa
    89221 taaagataac tggcgtgtta ctgcagaccg tatgcgtttt gatgatatcg atttgctgcg
    89281 tctctccgca cgcgaacggc gcaaactggt tggccacaac gtgtcgatga ttttccagga
    89341 accgcagtcg tgtctagatc cttcagaacg tgtgggtcgc cagttgatgc aaaacatccc
    89401 agcctggacc tataaaggcc gttggtggca gcgttttggc tggcgcaaac gccgtgccat
    89461 tgaactgctg caccgcgtgg gaattaaaga tcataaagat gcgatgcgca gttttcccta
    89521 tgagttgacc gaaggtgaat gtcagaaagt gatgatagcc attgcactgg cgaatcaacc
    89581 gcgtctgctg attgctgacg aaccgacaaa ctcaatggag ccaacaaccc aggcgcaaat
    89641 ctttcgcctg ctgacgcgtc taaaccaaaa cagcaatacc actattttgc ttatcagcca
    89701 tgacttacaa atgcttagcc aatgggcgga taaaattaac gtgctttact gcggtcaaac
    89761 ggtggaaacc gcgccgagta aggagttagt gacgatgcca catcatcctt atacccaggc
    89821 gctgatccgc gcgataccag acttcggaag cgcgatgccg cataaaagtc gcctcaatac
    89881 gctacccggc gctatcccac tgcttgaaca gttaccgatt ggttgtcgtc tggggccacg
    89941 ttgcccgtat gcacaacgag aatgcattgt gacgccacgt ttgacggggg cgaaaaatca
    90001 tctctatgcc tgtcatttcc cgctgaacat ggagaaagag tgagatgatc gaaacgctgc
    90061 ttgaagtgcg taatttaagt aaaacgttcc gctaccggac cggttggttt cgtcgtcaga
    90121 ccgtagaagc ggtaaaaccc ttgagcttta cgctacgtga aggccagaca ctggcgatta
    90181 ttggcgagaa tggttcgggt aaatccacgc tggcaaaaat gctggcggga atgatagaac
    90241 ctaccagcgg cgagttattg attgacgatc atccactgca ttttggcgat tattccttcc
    90301 gcagtcagcg tattcgcatg atttttcagg atccttcgac ctcgttgaat ccccgtcaac
    90361 gtatctcgca aattctggat tttccactgc gcctgaacac caacctggaa ccggaacagc
    90421 gtcgcaaaca gattattgaa acgatgcgta tggttgggct gctaccggat cacgtcagtt
    90481 actatccgca tatgttggcc cccgggcaaa aacagcgtct gggtctggcg cgcgcgctga
    90541 tattgcgccc aaaagtcatt attgccgatg aagccctcgc ttcactggat atgtcgatgc
    90601 gttcgcagtt gattaatctg atgctggaat tacaggaaaa acagggcatt tcgtatattt
    90661 atgttaccca gcatatcgga atgatgaagc acattagcga ccaggtgctg gtgatgcatc
    90721 agggcgaggt tgtcgagcga ggcagcaccg cggatgtgct ggcatcgccg ctgcatgaac
    90781 tcaccaaacg gctgattgcc gggcactttg gcgaagcatt gacggcggat gcgtggcgta
    90841 aggaccgtta gtgttctgca cctcatcctg acaggggaac ccccctcagg atgaggtatt
    90901 cattactttt gattgtcgat tctggtgaga gcaaccagga atgccagtgt ataacacacc
    90961 gtaattgcca gactcatttt taacagtgtt tctccgccaa atcccgacaa tggcgcggct
    91021 ataccgccga agacaaacat gctcatcccc atcaacgcag acgccgttcc agattcctgc
    91081 gtccctactg cactcatagc ctctgccccg ccaacagtgc cgatgccgct acaaaatgca
    91141 atagtgaaaa ataatgccac cagtgccgga atgggcaatt gtgtccatgc gcataagacc
    91201 gtgagcaatg cacacaaaat tgccgctatc aggccacctc gcaacaatgt catcgcatta
    91261 atccgtcgcg ccagacgcga aaagatccat gaagcaatga taagtccgat gccgttaagg
    91321 ccaaacacca ggctaaattg cattggacta aagccaaatt ccttctgcaa cacgaacgac
    91381 gaagagccga tatacgcaaa caagcctgcc agcataaaac tttgaatcag gcaaaagcgc
    91441 ataaaacggc ggttctgcac cacgcttcgt ccggttaata gcaatgatga gcccctttta
    91501 ttttctggca aggtctcatt aataaataac acgcagccca gcaacaatac ggtgctaatt
    91561 tcagccatta cccagaataa agtgcgccag tcaaaagtgc taacaatgta cccgcccagc
    91621 actggcgaca acaccggtgc caggccattc accgtcatta acagcgcaaa aaactgggtc
    91681 aacgttactc cctgatattt gtcacgagca atagaacgag agagcaccga accacccgcc
    91741 cccgcgatcc cttgaataaa acgccagacc accagccagt aaatattgtt cgttgtcgcg
    91801 cacaaaatgg aagaaacaat aaacaatagc aacgacaaga ttaacggacg tttacgccct
    91861 attttgtcgc tcagagggcc aaataacagt tgtccgacgc caagaccgat cagtgatgca
    91921 gtcagcgtta attgcgttat ggtcgtggtt gccgccagtt gctcagacat ctccggcaaa
    91981 gccggcaaat aaagatcggt acacatcggg ccgataccgg ctaaaagacc aagaatcaat
    92041 gcccatgaaa gagagactct agccatatta ttccttactt gagtgatgac atgccgccgc
    92101 ccagcgactg ccagagcgtg attcggtttg taaaatcagt cagttgcagt gccagcagct
    92161 cctgctgtgc gctccacaag ctgcgctgcg ccgtcaacac ggtcaggtaa tcaccaacac
    92221 ccgtctgata gcgacgcaag ccaacatcga ccgtttgttg ctcagcttta acatactggc
    92281 gctgtgcatc cagttgctct tcaagcgtgg tccgccgcgc cagtgcgtta gcgacatctt
    92341 taaatgctct ctgaacggtt ttttcatagg tggcaatgag tccacgcttt tgtgcttctg
    92401 cgtaacgaag ctgcgccaga ttacttccgc cagtgaacag cggtaatgtg accgatggcg
    92461 taaatgacca gatctgcatt ccgtggctga acaacgacga caatgcgtca ctgccaacac
    92521 cagcgctggc agtcagcgaa atcgtcggga aaaagttagc ccgcgccgcg ccaatatcgg
    92581 cattggcgct cttcaaatta tgttccgctt cctggatatc cgggcgacgc agcagaacgt
    92641 cagatgacac tcccgcagga accagactta tcatctgttc cggcaggctt tccagtgttc
    92701 ccggtagcaa attttcctca agcgttgtac ctgccagtaa gttcagcgca tttttatcct
    92761 gcatcacttg tgtctgatag ctggcaacgc tggcgcgcgc ttgctgataa acactcatcg
    92821 cctcactgac atctgtcgct gccgccgtgc caacctgttg ctggcgctgg ataattttca
    92881 atgagttttc tgcactggtc atcgtttctt ttgccagggc caggttgctg ttatcggccg
    92941 ccagtgtcag ccaggcggtg ctgatttcag caatcagcgt taaacgggtg ttctgggctg
    93001 taaattcact ggcaagccag gtttctcgcg ccgcccgaga taaactctga ttacgaccaa
    93061 acagatctaa cgtaaaactg gacaccgtgc cgtcagcctc ggcagtggtt tccgtaccat
    93121 tcgccagcga acgactgcgg gttgaactta aggccgcatt caccgtcggg aataacgacg
    93181 cattagtttg cccatagagc gcccgtgctg agtcaatatc agcaatcgct ttttgcacat
    93241 cgcggttact gttcagtgca atagtcacca cctgctgcaa tcggggatcg tggatcacct
    93301 gctgccagtc atggctgatt gcctttccct ggccctgtgc tcccggcaag gtcgccggga
    93361 ttggggattc aggcgtgctg tagtggggat cgagcgagac acatcccgca ctcaacagca
    93421 ccagaaaaat aagagaacga cgcaacataa cttactcctg acggcgcgat ttaccggcaa
    93481 acaaacgctt caccagtaca aaaaacagag gaacaaagaa aatagccagc aacgtagcgg
    93541 tcagcgtacc gccaataatg cccgtaccaa tggcgatgcg gctgttcgcc cctgagccgg
    93601 ttgctatcgc cagcggcatt accccccgca ataaacgcca gcgaggtcat gatgattggg
    93661 cgtaaacgag tctgcgccgc gcgtaatgcc gcacgactca aggaataacc ctctgcaacc
    93721 gccgcttcag caaattccac aatcagaatg gcgtttttcg acgacaagcc gatagtggtt
    93781 aatagcgcca cctggaagta aacatcgttg tttaaatcac gcatccaggc cgccagcgcc
    93841 gcgccaagaa gccccagagg gatcaccaga atgaccgaga acgggactga ccagctttcg
    93901 tacagtgctg caaggcagag gaaaaccacc agaatagaaa ctgcatacag actcaatgcc
    93961 tgaccgctgg ccagtttctc ctgcaatgac aaaccactcc aggcccaggt tgttcccgca
    94021 ggaagactgt ttgccagttc ctccattttt gtcattgcat cgccggaact aaagccagtc
    94081 gcattttccc cctgaatttc ataggctgca gaaccgttgt agcgcaccag tctttccggt
    94141 ccatacagcc agcgggtggt cgcaaaagca gagaatggtg tcatggcgtt atcactaccg
    94201 cgcacaaacc atttacctaa gtctgacggc gcggaacgaa attcactgtc gccctgaatg
    94261 tagacttttt tcacacgacc acggtcaata aagtcattca cataagtacc gccccacgca
    94321 ctggacaggg tgtcggtgac atcattcaaa ctcaacccta atgacaccgc tttattacta
    94381 tcaatatcta cctgcaattg cggcatttgt ggcaaatcat tggcacgaac agaatgtaac
    94441 tccggacttt gattcgcttt ttctatcaac tgattacgca tttgcagtag tgtttcgcga
    94501 tcggttccac cgttagctaa caattcaaac gtaaaaccat tgctttgccc cagcccatca
    94561 acggctggcg gcgtcatcgc aaataccgtg gcatcacgaa ttgtgcccag ctctttggtt
    94621 gcccgtaggg cgatagcctg ggcggtgttt tctgcccctt tacgttgaga ccagtttttc
    94681 aacgaaacaa acgccatccc ggtgttctgt ccgctgccgc taaaactgaa accatcaacg
    94741 gtaaaaatga catcggtatt tgctttctcg ttaatcagga accagtcaac aatctggcga
    94801 ttcacttctg ctgtacgggc agcggtcgcc ccggccggca gcgtgtactg caccatgatt
    94861 tcgccctggt cttcagtggg taagaaactg cccggcagtt tccacatcat cagggccatt
    94921 ccaccaccca gtacgagata aagccccatc gttcgagcag cacggcgcag gacataaatt
    94981 acgcctcgct gatatttatc ttcagtacgg cggtaaaagc ggtcaaatgc gccgaaaaag
    95041 ccttttttat gtggcggaac atgctggagg acggaaccgc acagggcggg agtcaaggtc
    95101 aatgccacca ccacggaaag cagcattgcg gagataatgg ttatcgaaaa ctgacgataa
    95161 attacccccg tagatccccc aaagaaggcc ataggcagga atacggctga caacaccagc
    95221 gcaatggcaa ccagtgcgcc agaaatctcg cacattgatt tttccgtggc ttcacgcgcg
    95281 ggcaaccctt tgtcacgcat aatgcgttcg acgttctcca ctaccacaat ggcatcgtcc
    95341 accagcaagc cgatcgccag caccatagca aacaacgtca gggtattaat cgaatagcca
    95401 aacaacgcaa gaacgccgaa tgtgcccagc aaaacgacgg ggacagctaa cgccggaatc
    95461 agtgtggcac gcaggttttg caggaataaa tacatcacgc aaaccaccaa tacgatggct
    95521 tcaaacagcg tctgaattac gtcctctaca gagattttaa taaattcagt gctgtctttc
    95581 gggtaagcga tgtcgtatcc ctgcggcatg ttccgctgga attcggcaat cttatccttg
    95641 accagcgtcg ccgtattcag cgcattcgca ccgggcgaaa gcattacagc aaccccggca
    95701 gacgggtgac cgttaagttt gccgatagcg gtataatctt cactgcccat ctcaacgcga
    95761 gccacatctt ttatacgaac aactgcgctg tctgactggc ttttcacgat aatatttttg
    95821 aactgttcca ccgtctgcaa gcgagactgc gcacgaaccg ttgcggtcag ttgctgagta
    95881 ttcggtgaag gcaatgcccc tattttcccg gcagtaacct gcacgttttg cgcttcaata
    95941 gcactttgca cgtctgaagg cattagcgag taagacgcca gtttggccgg gtcaagccag
    96001 atgcgcatag cgtattccgc gccaaagact tgcagactcc ccacaccttc aacacgcgcc
    96061 agcgggtcct gaacgttact aaccaaccag tcggcgatat ccgaactgga ggctttgtca
    96121 gtggtgtcat acacggcagc aatcagcaaa aagttgcttt gtgatttctc caccgtaaca
    96181 cccgtttgct gcacttcgct gggtaggcgc gactccgcct gctgaatttt attctgcacc
    96241 tgaacctgtg cagtatctgg atcggtacct tgttcaaagg tcacattaat actgaccgaa
    96301 ccatcagagc tgctggttga gctgaaatag agtaaattat caagcccagt gagttgttgc
    96361 tcgatgacct gagtcacgct gttctccagc gtttcggcag aagcaccagt ataagtggct
    96421 gaaattttaa tggtcggtgg cgcaacgtca ggatattgcg cgacgggcaa tgtgcgaata
    96481 gccagaatcc ccgccaacat aatcaaaatg gcgataaccc aggcaaagac cgggcggcga
    96541 acaaaaaagc gcgaaaacat caggcgttgc ctccattagc ctgaacttca acagccttga
    96601 ccgtctgccc tgaagtgact ttggcagaac cttcaacaat cagtcggtcg ccgctgtgca
    96661 ggccgtttag caccagccat ttatcaccat atgtttctcc cgtttcgagc gttcgctgct
    96721 ctactttatt gtctttattc accaccagcg cagttgcatt gcctttagca tcgcgcgtga
    96781 cgccctgttg cggcgcgaga atagcgtctt ccatcacgcc ttcatcgaca cgagcgcgaa
    96841 caaacattcc cggtaataat tgctgttgtg gattggggaa aatcgcccgt aatgtcaccg
    96901 aaccggtaga ctcatctaca gcgacttcgg tgagttccag acgccctttt tcgctgtagg
    96961 ttgtgccatc ttccagaata agtgagacgc tcatggtgtc actgttggtc gccagtgact
    97021 gtttacgcag acgtaataaa tcgacactgg agcgagtgag gtcgacatac attgtatcca
    97081 gaccacgaat agtcgttaac gctgtatctt gcgacgcggt caccagtgcg ccaggggtta
    97141 ccgacgaaat gccaatgcgc cctgaaatcg gtgcggttac cgtggtccag tcaagattaa
    97201 tgcgcgcagt ttccagtgcg gcttttttcg ccgctacact ggctttatct tgtgcacagg
    97261 tagactgagc atcatcagca tcctgttgtg aaacaccgtt ctctttcacc agtcgggcat
    97321 aacgctgcgc tttctggcaa tcggctttta ccagtgcctg tgcttgttgt aatgctgccc
    97381 gagcttcatt ccatgcagcc tgataactgg ccgcatcaat ctggtagagc ggctgtccag
    97441 ccttgaccag atcaccttcc ttaaataagc gtttctggat aattccccca acctgcggac
    97501 gtacttcggc actgagcgca gcactggtgc gtccggttaa ttcactgact accgagaccg
    97561 gttgagattt gactgtaaca acgccgactt ctgtttctga cggggatgaa ttgttacttt
    97621 gcgtattatc acaaccagat aagagcagca ttgccactac agatgttgct atatacttca
    97681 ttattttctc cagggtgaga aataccctcc tcattaaaat taatgaggat atgtcaggga
    97741 tattcagtta taacgaaccc gcatcgcgaa atgaatttcg caatgaaata agggggatta
    97801 atatcccccg ctattggtta attctgttct tatttattta tcaacatact aacaatatcc
    97861 tgatatattt cctgcagatg ttgtggatcg cttttttgcg gtgttaaacg acgataaacc
    97921 gtaccttcca tcatggtagc aaacacttca acgcagcaac gaatgtgctc ctcgctaaga
    97981 tggggatgca ttcttttcat atgtgcacaa ccgttagcga acattctggc gtcagcttct
    98041 tcaagcattg ccataacccg cgggttgcgt gtggcttctg cagccacttc cagcatcagc
    98101 gcgtcgtcgt cctcatttaa ggatttacgc aaggcaagga cttccgggaa gtgatcggta
    98161 cgcgcgtcaa tgtccatttg ggcaatacga aagtcgatga tacggcgaac catctcttca
    98221 ataatggcgt ctttgttggc gaagtaacga tatatctgac ccacgctaag ctgagcttcg
    98281 ctggcaattt gcgacatact ggcggcatga aacccgcagc gacgaaaaca ccgacgggcg
    98341 gcgttaatga tttcatcctg acgctgtttg tggcgtattt ccagcttgct ggtcatttct
    98401 gtctcctggc ctatcacttg gtgagaatga tcgttctcat ttttgctgat tatacgtttc
    98461 tccaaagtaa tggaaagtat ttttatctta ttcatggtgc atattctgat aatgaagaga
    98521 ttgtggagat tctgtgaaga tagttgacaa tattaagttg gggtcaattc atcagaaagg
    98581 ctgctacttg ttgtcgccag ttctgttttc atcatcaaac ttatcggagc taatacgaat
    98641 agtttcatag tcagattcta taagatattt ccccaaccta ccccttgcag gcgtcaagag
    98701 tagataacgc aaaatgctct gtaccgcagc ttctctccgg tacagaaagc gcaactatag
    98761 ccacccacag ccaggttgat tataataacc gtttatctgt tcgtactgtt tactaaaacg
    98821 acgaatcgcc tgattttcag gcacaacaag catcaacaat aaggattaaa gctatgggtt
    98881 ttctttccgg taagcgcatt ctggtaaccg gtgttgccag caaactgtcc atcgcctacg
    98941 gtatcgctca ggcgatgcac cgcgaaggag ctgaactggc attcacctac cagaacgaca
    99001 aactgaaagg ccgcgtagaa gaatttgccg ctcaattggg ttctgacatc gttctgcaat
    99061 gcgatgttgc cgaagatgcc agcatcgaca ccatgttcgc tgaactgggg aaagtttggc
    99121 cgaaatttga cggtttcgta cactctattg gttttgcacc tggcgatcag ctggatggtg
    99181 actatgttaa cgccgttact cgtgaaggct tcaaaattgc ccacgacatc agctcctaca
    99241 gcttcgttgc aatggcaaaa gcttgccgct ccatgctgaa tccgggttct gccctgctga
    99301 ccctttccta ccttggcgct gagcgcgcta tcccgaacta caacgttatg ggtctggcaa
    99361 aagcgtctct ggaagcgaac gtgcgctata tggcgaacgc gatgggtccg gaaggtgtgc
    99421 gtgttaacgc catctctgct ggtccgatcc gtactctggc ggcctccggt atcaaagact
    99481 tccgcaaaat gctggctcat tgcgaagccg ttaccccgat tcgccgtacc gttactattg
    99541 aagatgtggg taactctgcg gcattcctgt gctccgatct ctctgccggt atctccggtg
    99601 aagtggtcca cgttgacggc ggtttcagca ttgctgcaat gaacgaactc gaactgaaat
    99661 aatcgttctg ttggtaaaga tgggcggcgt tctgccgccc gttatctctg ttataccttt
    99721 ctgatatttg ttatcgccga tccgtctttc tccccttccc gccttgcgtc aggataacga
    99781 tttcctttac gaccaaggag cgcccatgga acaacgccac atcaccggca aaagccactg
    99841 gtatcatgaa acgcaatcca gtactacgga gtatgacgtt ctgcctctgg tcccggaagc
    99901 cgcaaaggtc agcgatccct ttctactcga cgtgatcctt gaaaaagaaa cgctggcccc
    99961 cttcctttca tggctggacc ctgcgcgtgt tcttgcagtg gagttgttcc ctgaccagct
   100021 taccgtgacc cgttcacaga ccttcaccgc ttatgaacgc ttgtcgacgg ccctgacggt
   100081 tgctcaggtt tgcggcgtcc agcggttatg taactactat tcggcgcgac ttacgccgct
   100141 ccccgggcct gattccacca gggaaagtaa tcatcggttg gcacaaatca cgcaatatgc
   100201 ccgccaactg gctagctcgc cttctattat cgacaaccga tcgcgccagc atctgaatga
   100261 cgtcggtctt actgcctggg actgtgtgat cattaaccaa atcattggtt ttattggctt
   100321 tcaggcgcgg acaattgcga catttcaggc ttatctcggg catccggtac gctggttacc
   100381 cgggctggag atacaaaact acgccgacgc gtcactgttt gctgatgaat cattacgctg
   100441 gcgaagcagc tatgaggtgg aaaaactacc tgaagaacac acaaaaagtt caactgcaga
   100501 actttgccaa ctggccgaaa tactctctct ccaccctatt tcactttccc ttctcgaaag
   100561 gttgttaaac agcacacggg ttaatacaca gccggataat cagcttgcgg cgttgttatg
   100621 cgcgcggata aatggcagtc ctgcttgttt tgccgcctgt atggattcat caaatgaata
   100681 taaaaaaatc agcccccttc tgcgcaaggg cgaaaatgaa attaaccaat gggctgaccg
   100741 tcattctgtt gagcgcgcta ccgttcaggc gatacaatgg ctgacccgag cacccgatcg
   100801 ctttagcgcc gcccagttca gccctttact cgaacacgaa aaatcatcaa cgcagattat
   100861 taatctgctg gtatggagcg ggctgtgtgg ctggataaat cgcttaaaaa tcgcgttggg
   100921 tgagacatat taaccttgcc gcgtcagaca gattcgcgta aaactgtcag ccgctctaat
   100981 ggccaccaaa atagacaatt atgtttcagg acaacccgct gctagcgcag cttaaacagc
   101041 aactgcattc ccagacgcca cgcgctgaag gggtggtaaa agccacagaa aaaggttttg
   101101 gcttcctgga agtcgacgcg caaaaaagtt atttcattcc gccgccgcag atgaaaaaag
   101161 tcatgcatgg cgaccgaatt atcgcggtga tccacagtga aaaagaacgt gaatccgcag
   101221 agccagaaga actggttgaa ccgtttctga ctcgtttcgt gggtaaagtt cagggcaaaa
   101281 atgaccgtct ggccatcgtt cctgatcatc cactcttaaa agacgccatt ccttgccgcg
   101341 cagcccgtgg tctgaaccac gagtttaaag aaggcgactg ggcagttgcc gaaatgcgcc
   101401 gtcatccgct gaaaggcgat cgttctttct atgctgaact gacacaatac atcacttttg
   101461 gtgacgacca ctttgtaccg tggtgggtta cccttgcgcg ccataatctg gaaaaagaag
   101521 caccagacgg cgtcgctacc gaaatgctcg atgaaggtct ggttcgtaaa gatctgaccg
   101581 cgctggattt tgtcaccatc gacagtgcca gcacagaaga tatggatgac gcccttttcg
   101641 ctaaggcgtt gccggatgac aaacttcagc tgattgtggc gattgccgat ccaaccgcgt
   101701 ggattgctga aggcagcaag ctggacaaag ccgcgaaaat tcgcgcattc accaactatc
   101761 tgcctggctt caacatccct atgctgcctc gcgagctttc tgacgatctc tgctcactgc
   101821 gcgccaatga agtccgcccg gtactggcat gccgcatgac gctctccgct gatggcacca
   101881 ttgaagataa tatcgaattc tttgccgcca ccatcgaatc caaagcgaag ctggtgtatg
   101941 accaggtttc tgactggctg gagaataccg gtgactggca gcctgaaagt gaagcaattg
   102001 ccgaacaagt ccgtttgcta gcgcaaattt gccaacgccg cggcgagtgg cgtcataacc
   102061 acgcactggt gtttaaagat cgcccggatt accgctttat tctcggtgaa aaaggtgaag
   102121 tgctggatat cgtcgccgag cctcgtcgca ttgccaaccg tatcgtcgaa gaagcgatga
   102181 ttgccgctaa catttgtgca gctcgcgtac tgcgcgataa gctcggtttt ggtatctata
   102241 acgtgcatat gggctttgat ccggcgaatg ccgacgcgct ggcagcgttg ctgaaaacgc
   102301 acggtctgca tgtcgatgcc gaagaagtgc tcacgctgga cggtttctgc aaactgcgtc
   102361 gtgaactgga tgcgcaacca actggtttcc tcgacagccg cattcgtcgc ttccagtcat
   102421 ttgctgaaat tagcactgaa cccggtcctc actttggcct cggtctggaa gcatacgcca
   102481 cctggacttc gccgatccgt aaatatggcg acatgatcaa ccaccgtctg ctgaaagcgg
   102541 ttatcaaagg cgaaactgcg acgcgtccac aggatgaaat cactgtccaa atggccgagc
   102601 gtcgccgtct caatcggatg gcagaacgtg atgttggtga ctggttatac gcacgcttcc
   102661 tgaaagacaa agccgggacc gacacccgtt tcgcagcgga aattgtcgat atcagccgtg
   102721 gcggcatgcg tgttcgtttg gttgataacg gcgctatcgc ctttattcct gcacctttct
   102781 tacacgctgt gcgcgatgaa ctggtttgca gccaggaaaa cggcaccgta caaattaaag
   102841 gtgaaacggt ttataaagta actgacgtta ttgacgtcac cattgccgaa gtccgcatgg
   102901 aaacccgcag cattattgcg cgcccggtcg cgtaatctcc tttcacggcc cattccttat
   102961 gaatgggccg tttatttccc cgctctacct tcatcatatc ccggcagttt tttaactgac
   103021 tttcgtttga aaactggcgt ttttccaaaa ctagattaca aataaatata taaaaaaaac
   103081 aatgtgttta gccgccgtta atttcatgta agtcatgttg ttccttttat ttaaccgcgg
   103141 actccgctgt taaccggagg atatgcatca tgaaaaccgt tagggagtcc acaacgttgt
   103201 acaactttct cggatcgcac aatccatact ggcggttgac ggaaagcagc gatgttttgc
   103261 gcttttctac caccgaaacc acagaacctg aacgtacgtt gcagttatct gccgaacagg
   103321 ctgctcgcat cagggaaatg acggtcatca cctccagcct gatgatgagt ctgaccgtcg
   103381 atgagagcga tctttctgtg catctggtag gacgaaaaat caataaacgg gaatgggctg
   103441 gcaacgcgtc tgcatggcat gacacaccag cagttgctcg tgatttatca cacggacttt
   103501 cctttgctga gcaggtagtt tctgaagcac attccgcaat agtgattctc gacagccggg
   103561 ggaatatcca acgcttcaat cggttatgtg aagattacac agggttgaac gaacacgacg
   103621 tcattggcca aagcgtgttt aaactgttta tgagccgtcg tgaagctgcg gcatccaggc
   103681 gcaataaccg tgtatttttt cgaagcggca atgcatatga agtcgaactg tggataccaa
   103741 cacgtaaagg ccagcggctg tttctgtttc gcaataaatt tgtccacagc ggcagtggca
   103801 aaaacgagat ttttttaatc tgttccggca ccgacattac cgaagagcgc cgcgctcagg
   103861 aacgactgcg tattctggca aataccgaca gtatcaccgg actgccgaat cgtaacgcaa
   103921 tgcaggagtt aatcgatcac gctattaatc aggcagataa caataaagtt ggggtcgtgt
   103981 atcttgattt ggataatttt aaaaaggtca acgacgccta tgggcattta tttggtgacc
   104041 agttattacg cgacgtgtca ttggctattt taagctgtct cgaacatgac caggtgttgg
   104101 cgcgtccagg tggcgatgag tttctggtac tggcatccaa cacctcacaa agcgcgctgg
   104161 aagcaatggc atcacgaatt ttgacccgct tacggctccc ctttcgcatt ggtttaattg
   104221 aagtttatac cagctgttca gtaggtattg cactctctcc cgaacatggt tcggacagcg
   104281 cggctattat tcgtcacgcc gacacagcaa tgtacacagc aaaggaaggc ggacgaggac
   104341 aattttgcgt ttttacccca gaaatgaatc aacgggtatt tgaatatctc tggctggata
   104401 ccaacttgcg taaagcactg gaaaacgatc agttggttat tcactatcaa cccaaaatca
   104461 cctggcgtgg cgaagtgcgc agtctggaag cactagtacg ttggcagtca cctgaacgtg
   104521 ggttgattcc accgttggac ttcatttcct acgcagaaga gtcagggcta attgtgcctt
   104581 taggccgttg ggtgattctc gatgtcgtac gccaggtggc gaagtggcgg gataaaggca
   104641 ttaacctgcg agtggcggta aacatttctg cacgtcagct cgccgatcaa accattttca
   104701 ccgccctgaa acaggttctc caggaactca attttgaata ctgccctatt gatgttgaat
   104761 tgacagagag ttgtctgatt gagaatgatg aactggcact gtctgttatt caacaattta
   104821 gccaactagg tgcgcaagtg catctggatg attttggtac cgcctactct tcactttcgc
   104881 aactggcgcg ctttccgatc gatgccatca aacttgacca ggtttttgtt cgagatattc
   104941 acaaacaacc tgtctcgcag tcactggttc gggcaatcgt cgctgtggcc caggcattga
   105001 atcttcaggt gatcgccgaa ggtgtagaga gtgcaaagga agatgctttt ttaaccaaga
   105061 atgggatcaa tgagcggcaa ggatttttgt ttgccaaacc gatgcccgcc gtcgccttcg
   105121 aacgctggta taaacgctat ctgaagcgcg cataagtaga tgaatatata tacttagcgc
   105181 tatattccgt cattatatat cgtattcccg gatgataatt agccgttgct gttaaacact
   105241 tctcttttag cggcaatttt tgcattattc attttccccc ttttgctata aggatgtcct
   105301 tgatgtctga atttgatgcc cagcgcgttg ccgaacgtat tgatattgtg ttagatatcc
   105361 ttgtggcaga cgattaccac tctgcgatcc ataatctcga gatacttaaa gcagaactct
   105421 tgcgccaggt tgctgaatct acgccagaca tcccgaaggc accgtgggag atttaacaat
   105481 cgtgccttca ggttttccta tttgaggatg cccggcgcgt cagtattttt ttatttagta
   105541 ttataacgtt ataagaatta cagcgatgaa ttcccgacaa caaactattc tacagatggt
   105601 cattgaccag ggtcaggtta gcgtaaccga tctggcaaaa gccactggag tttctgaagt
   105661 taccattcgc caggatctca acaccctcga aaaactgagt tacctccgcc gtgcacatgg
   105721 ctttgcagtt tcgcttgata gtgatgacgt cgaaacccgt atgatgagca actatacgct
   105781 gaagcgtgaa ctcgccgagt ttgccgcgtc actggttcaa ccgggcgaaa ccatctttat
   105841 cgaaaatggc agcagcaatg ccctgctcgc ccggactctg ggggagcaga agaaaaatgt
   105901 cactatcatc acggtcagca gctacatcgc gcatttgctg aaagacgcgc cttgtgaagt
   105961 tattttgctc ggtggcgtgt accagaaaaa aagcgaaagt atggttggcc cgttgacacg
   106021 ccagtgcatc caacaggtac atttcagcaa agcatttatt ggtattgatg gctggcaacc
   106081 tgaaactgga ttcaccggtc gcgatatgat gcgtaccgat gtggtgaatg ccgtgctgga
   106141 aaaagagtgc gaagcgatag tcctgactga cagctcgaaa tttggtgctg tacattcata
   106201 ctccatcggt cccgttgagc gattcaatcg cgtgattacc gattcgaaaa tacgcgccag
   106261 cgatctgatg catcttgagc acagtaaact caccgttcac gtcgttgaca tttaaccatt
   106321 tcccttctct acggatgatt tgcagtttga caaatcatcc gctctaagat gattcctggt
   106381 tgataattaa gactatttac ctgttattaa cactctcaag atataaaatt attatcagcg
   106441 atataacagg aagtcattat cacctgcgtg atataaccct gcgcgcgagc ggatttcacg
   106501 gaataatttc accaggctta ttcttagcta ttatagttat agagagctta cttccgtgaa
   106561 tcataaattc aggagagagt attatgtttg taacgagcaa aaaaatgacc gcggctgttc
   106621 tggcaattac cctggcaatg tctctgagtg cctgttctaa ctggtctaaa cgggaccgca
   106681 acaccgcaat cggcgcgggt gcaggggcat taggcggtgc agtactgacc gatggcagta
   106741 cgttgggtac attaggtggc gcagctgtcg gtggtgttat tggtcaccag gtcggtaaat
   106801 aatcgtaaat aaatgtcgat tatcgactcg taattataat acgcttgatt ctgttcagta
   106861 cgaaataact gaataataca cacagtaaaa tattcgaaag ccacggatat atccgtggct
   106921 ttttatgtta accgcctgcg agttttacct tcatcccttt cgcttcaagt agtgatttta
   106981 ataaatcacg cttatcgccc tggatttcaa taactccatc tttaactgct ccgccgcagc
   107041 cgcatttttt cttcagttcc gctgcaagtt ttgtcagttc ggcatcatcg agatcgatac
   107101 cggtaatcag gcaaacgccc ttacctttac gtccactggt ctgacgctga atacgcacca
   107161 caccatcgcc tttagagcgt actggggccg ctttgggctc atcaatacgc ccggtttctg
   107221 ttgagtagac cagacggctg ttggaatcac tcatcatgca ctccgctgta aagaggcgtt
   107281 gatcgctttc agcgtctgcg ctggatctac cgattgcgtt accgggcgac caatcaccat
   107341 ataatcaaca ccagccgcca acgcctgttc tggcgtcata atgcggcgct ggtcaccagc
   107401 ttcactcccc tgcggacgaa tgcccggcgt aaccagtttg aactcctgac cgaatacctg
   107461 tttaaagcgc acagcttcct gagcagaaca caccacacca tcaaggccac atttttgcgt
   107521 cagtgctgcc agacgttctg cataatctgc aggtgacagt gtcacgccaa gatcggccag
   107581 gtcgctggct tccatgctgg tcaacactgt cacagcaatc aaaagcggtg catctttgcc
   107641 aaacggaacc agtgcctcac gcgctgcggt catcatacgc gccccaccag aggcatgaac
   107701 attcaccatc cacacgccta agtcagctgc agcagcgaca gcgtgcgctg cagtgttggg
   107761 gatatcgtgg aatttcaggt caagaaagat atcaaaacca cgctgttgaa gttcgcgcac
   107821 aaactgtggc ccaaacaatg taaacatctc tttgccgacc ttcagacgac aatcgcgtgg
   107881 gtcgatcttg tcgacaaagg ccagcgcggc atcacgatta tgataatcaa gggcaacaac
   107941 cacaggagaa ttcgtaacag cgcgggaaga agatgaagca gttaacgtca tgaccagacc
   108001 ttcttgatga tgggcaccgg aagtggcgcg aaacaggtaa acggcgagca ttctacccgt
   108061 ggaaataaaa aattaacagt tgcgatttct cccctggcag gctttttctg cgccgccgtt
   108121 gccggagcac aatgggacat aaataactag tatgttgtaa ctaaagtcgg tttttttaaa
   108181 aaattacagg ccatcaagac cgcgaatcgg tttaatagtt gaccaggcac gacaagacgg
   108241 acaatgccag tagagggtgt atgcggtaaa accacatttc tggcagcgat aacgaggctt
   108301 actacgtacc ttctcgccaa ccatgtcacg cagcaccatc aggctctctt tggcacgccc
   108361 ttcttccgct tcatttaagt ggtaatccat taacttatgg aacacacgca tggtcggatg
   108421 acgctgaagc tggcgcgtaa tatagacctg tgcggcctca ctaccgtcgc gcgcttcgat
   108481 gatatcagca agcatcaatt cagcatcggc accggtgttc tcttccaccg cgcgctgcag
   108541 gaattctgcc cattcggcag ttttacccaa ctgctgatag caggtttgca gcatttccag
   108601 cgtttcgctg accagttctc tgtcctggga tatcacgcgt tgcaaacttt cgacggcttt
   108661 ggcgtattct cctttcgcca taaacacgcg tcccatcatg atggatacgc gggcgctgtt
   108721 tttatctgcc gccgcccctt tttttagcaa ggtcatggca cgatcgagat cgtcgctggc
   108781 catatgctgc agggctaact cacagtagaa atgggcaatt tcgacgcgct gtttatcttt
   108841 acccagcttc accaggcgtt cggcaacatc aattgctttc tgccactcac tggtagcctg
   108901 gtagatttgt agcaactgtt gcagcgcgcc aatgcggaag tcagtttcat cggtcagctg
   108961 attgaacatg tcttccgcgc ggtcatataa cccggcggcc atgtaatcac gccccagttg
   109021 ttgaatcgcc aacagacgct gttcataggt cagcgaggcg ctttccatta gggtctgatg
   109081 gatgcgaata gcgcgatcaa cttcgccacg cgaacggaac aggtttccga gcgtaaggtg
   109141 ggcttcaacg gtgcctgtat cctctttaag catatcgaga aacaggtcta ccgctttatc
   109201 ctgttgatta ctaagcagga agttaacccc cgctacgtaa tcacgcgaca agcggttggc
   109261 ttcatcttgc ttgttttgtt gcgcacttct gcggcccata taccagccat aggcagcggc
   109321 tacaggcaaa agcagaaaca acaactccag catagaaagt tattccttcg ccgctgacga
   109381 gtgctgcact acagccacgt cagtcgcggg tgaaagctgg ttttccagtc gctttatttt
   109441 acgttcagcg cgcaccaggg aaacacgaac tcgcagccag aacaggccac aaatcaacca
   109501 accgatagca aaacccgcag caaacaatac cgccagcaat gtggaaatac ggtactcccc
   109561 ttgcgctaac agataattaa acgtcacctg ttgatcgttc tgcgcaccca acgtgaccga
   109621 aatcacgaag atcgctaaca ccagtaagaa aatgagtaaa tatttcacat tacttcccgt
   109681 tatgtggtta gagcgaataa attatggtca acttaccgca atagcccttt aaagtaccat
   109741 tttcgcgccg tctgcgaaat ggcatccacg cgcattgttg atgatttaag ggctaaattc
   109801 ggaaaatcaa ccagcgttaa ctttcttgtt ctcgttgcgc tatttcgcga ttttcttccg
   109861 caggtggtgt taatggcccg caaattcgtt gcgcaagcca cgttgccacc gccaccagtg
   109921 cccacgaaat caacgtagct accaccagat cgcgtggcca atgcatcccg agcagcaggc
   109981 ggcttcccat gactcccgtt gcccagacca gcaagatagc aatggttaac gttcgccgac
   110041 gcggccacag caaaccaacg gccagcagtg cccaactggc agcaaacatc gtgtgaccgg
   110101 aaggaaaggc aaaccccgtc tctttctgcc agtgtgaacg caaatattgt ggaatatttt
   110161 tctcttcagc caactgttct ttcactagat ttccgcgttc tgctcgcttt aaagtgtaga
   110221 actcatcaac cggaatatga tgtgtttttt ccagccagat aacaaaaggt cgtggttcct
   110281 ggactttgtc tttgatccag gatttaacgc cttgtcccac aaggattgcg gccgccagaa
   110341 tggcaaataa tacaaaggca gccttaatgc gaaaacgcag acaccagaga aaccagccga
   110401 ataaaatcaa atgtgtaatg acgccccagg gctgggtgac agtttcagta acccaaaaag
   110461 ccgcttttag tagccaactt tgttctccag gttgccaacg ccagccagaa atccatacgg
   110521 ctactggcat aacaagcaat agtgcagctc ccactgcggt acgtcttgca atcgaacgca
   110581 tggcctctcc tttttgataa gtcccacaat cataactgaa aatgccagtt ccaggaaaaa
   110641 ttgacagatt tgtgccattc cgtgaacgat cgacgcgtcg tgattaggtg aaccccttct
   110701 cgttatggca aaataagcca atacagaacc agcattatct ggagaatttc atgcagctta
   110761 aacgtgtggc agaagccaaa ctgccaaccc catggggcga tttcctgatg gtgggatttg
   110821 aagaactggc aaccggacac gatcatgtcg cgctagtcta tggcgatatt tccgggcata
   110881 ccccggtact tgcgcgcgtc cattccgaat gtctgaccgg tgacgccctg ttcagcttgc
   110941 gctgcgattg tggcttccag ctcgaagcgg cattgacgca aattgccgag gaaggccgtg
   111001 gtattttgct gtatcaccgt caggaaggtc gtaacattgg tctgctgaat aaaatccgcg
   111061 cttacgcact gcaggatcaa ggttacgata ctgtagaggc taaccaccag ttaggcttcg
   111121 ccgctgatga gcgcgacttc actctttgcg ctgatatgtt caaactcctt ggcgtcaatg
   111181 aagtccgctt gttaaccaat aacccgaaaa aagtcgaaat tctgaccgaa gcggggatta
   111241 atattgttga acgcgtacca ttgattgtag gtcgtaaccc caataacgaa cattatctcg
   111301 ataccaaagc cgagaaaatg ggccatttgc tgaacaaata accctcttgc attgtgtaat
   111361 taatttgctt gccggaagca aaataaccgg caagcaaata cttgttactt caacatatta
   111421 cgaatgacat aatgcaaaat gccgtcgttc tggtagtaag tcaactccgt cgcggtgtcg
   111481 atacgacaac ggcagggtac gacttcctgg ctaccatccg cgcgcgtaag cgtcaccgga
   111541 accgtcgcgc cgggttgtag attttgcaga tcgccaatat caatcttctc ttccccggtt
   111601 agccctaacg ttttacgcgt tacgccttgc ggaaattcca gcggcaggat gcccatgcca
   111661 attaaattcg aacggtgaat tcgttcaaac gattcggcaa tcaccacacg aataccaagc
   111721 agacgcggac cttttgccgc ccagtcacgg ctggagcctg atccatactc tttcccggca
   111781 atcaccgcca gcggcgtttg ctcctgctta tagcgcatcg cagcatcata aatagagact
   111841 acgtcgctgt caggtaaatg ccgcgtcatc cccccttcaa cgccaggcac catttcatta
   111901 cggatgcgaa tattggcgaa ggtgccgcgc atcatcactt catggttacc acgccgcgaa
   111961 ccgtaggagt taaagtcttt tcgctcaaca ccccgacctt gtaggtatcg acccgctggg
   112021 ctgtcgggct taatactgcc cgccggagag atatggtcag tggtgactga atcccccagc
   112081 attgcgagga tccgcgcacc gtgaatatct tccacaggtg ctggcgttgc ctgcatttca
   112141 tcaaagaaag gcgataaacg aatataggtt gaatcctcct gccatccgta ggtatcggat
   112201 cgtgcgacgt taatttcttt ccactctgca gtgccttcaa agacttctgc gtactctttg
   112261 cggaacattt ctgtggagac ttgttctacc gcacgggcaa tttcttgtgc cgatggccag
   112321 atatctttca gatatattgg atcgccttta cgatcatgac caataggctc agaagccagg
   112381 ttgatattca tatttcccgc cagcgcatag gcaaccacca gcggcggcga ggccagccag
   112441 ttagttttaa ccagcggatg gatacggcct tcaaagttac ggttgccgga cagcactgca
   112501 ccgacggtta aatcgccttt tttgattgcc gtttcgatag gatcgggcag cggcccagag
   112561 ttaccaatac aggtggtaca accgtatccc acaaggttaa accccagttc gtcgagatac
   112621 ggtgtcagtt tcgcttttgc cagatagtca gaaacgactt tcgaacccgg tgccagcgac
   112681 gctttgaccc acggttgccg cttgaggcca agagttacgg cttttttcgc cagcaagcct
   112741 gcggccatca gcacacttgg gttagaagtg ttggtgcacg aggttatcgc agcaatgacc
   112801 acagcgccat caggtaactg atactgatgt ccgttcataa cataatcgac cggctggcga
   112861 tctttatgcg tggcattcac ttccagttcg ttactggcgg caaatgcttt tggtacatcg
   112921 ggcagtgcaa cgcgatcctg tgggcgttta ggccctgcca ggctcgcttc aacgtcattc
   112981 atatccagat ctaacgtact ggtaaaaatc ggttcatcgc ccgggttacg ccacatgccc
   113041 tgcgctttgg catatttttc gaccaactcg acctgatctt cgctgcgccc gcttaaacgc
   113101 atgtaatcga gggttacagc atcgattggg aagaagccac aggtggcacc atattctggc
   113161 gacatattgg caatggtggc gcgatccgcc aacggtagtg aatccagacc atcaccataa
   113221 aattcgacga atttccccac cacgccatgt ttgcgcagca tttgggtaac agtgagaacc
   113281 aggtctgtgg cggtaatacc ttcacgtaat tttccggtaa gtttaaagcc cactacgtcc
   113341 gggataagca tggaaaccgg ctggcctaac attgcggctt ctgcttcgat gccacccacg
   113401 ccccacccca gcacgccaag gccgttgatc atagtggtgt gcgagtcagt accaacgagt
   113461 gtatccggat aagcaatcca ttcaccgtcc tgcaattcgc tccacactgc tttgccgaga
   113521 tattcgaggt taacctgatg gcaaatgcct gtgcctggcg gcacgacgct aaaccgactg
   113581 aacgcttgct ttccccattt caggaacaca taacgttcgt ggttgcgctc catttccagg
   113641 cgtacgtttt cttcaaatgc ctcatcatca ccaaaacgat cgacggtcac cgagtggtca
   113701 atgaccaggt cgaccggtga gagcgggtta acctttgcag tatcgccacc gaggcgttta
   113761 accgcttcgc gcattgccgc cagatcaaca acggcaggta cgccggtaaa gtcctgcatc
   113821 agcacccttg ccgggcggta ggcaatttca cggtcagcat gggcattttt cagccatccc
   113881 gccagcgcgt ggatatcctc ttcggtaacc gagttaccat cctgccagcg cagcagattt
   113941 tcgagcaaaa ctttgagtga cttgggtaga cgggtgatat cgcccagtga tttggcagca
   114001 agcggcaggc tgtagtagtg gtaagtttta tctttggcct gcaacgcgtc cttactggct
   114061 tctcgtaggg ttgacgacat agctcctcct taatgacagg gttgcgaagc cctgattctc
   114121 ttcagggtga atattaaaga taacacaaac atcgcgtaac gatttgataa caacccaaat
   114181 tgataaaaga ggagaaacct tgtggcaaag caaatgacaa ccccgccgca gcggggtcaa
   114241 ggatcagtga gtaatgatcc agaggagttc ggcccagaag agcatcgaca gagaaaatgc
   114301 acccatccag gaccaatatt tcatatttgt gttgatcata ggctacaccc ttaacggtta
   114361 ttactttgtg ttgagataac tcaacctgtt attgctgcgg tgtattaaaa gccctttaat
   114421 tcattaaaaa tgggtaataa taatgatgat aattattgtt tcggctgatc atcttcagca
   114481 aacagttcta taaaggcttg ctgctgcgat gttagttgcc agtcgtctgg aataacgacg
   114541 gaacattccc gtttcgtttc gggttcattg tcactatgct ggcataatgg tgacgactcc
   114601 agttgctcct gaccaaccat agttatcccc acactttcca aatcagcagt gcgacaacca
   114661 cccagaaaag tgcagaacca agaaaaaccg ccagccaggc tttacgtttt aattcaggat
   114721 cgcggcgggg ttcctgattt ccagaaggca ttgctaacct catataatcg ccatcactta
   114781 tcagcaagac gttgaacgat tggtgtaatt aatcatacga tgagacaaat cctaaagaaa
   114841 ctttagtcaa aaatccagcg aatttacgaa tcgtctccag tgaataattc aatcttttga
   114901 cctgaaatag atatttgaaa actggaattt gcgaggcggg taattagaca ctactttaca
   114961 ggtaatttac cctataaata tgagggataa aa
//
LOCUS       DAZKIP010000002       100434 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZKIP010000002 DAZKIP010000000
VERSION     DAZKIP010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 100434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 100434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..100434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.002"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(138..713)
                     /gene="gmhB"
                     /locus_tag="ACHOUK_000104"
     CDS             complement(138..713)
                     /gene="gmhB"
                     /locus_tag="ACHOUK_000104"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFP9895636.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
     gene            901..1932
                     /gene="metN"
                     /locus_tag="ACHOUK_000105"
     CDS             901..1932
                     /gene="metN"
                     /locus_tag="ACHOUK_000105"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFP9895637.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            1925..2578
                     /gene="metI"
                     /locus_tag="ACHOUK_000106"
     CDS             1925..2578
                     /gene="metI"
                     /locus_tag="ACHOUK_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFP9895638.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVVGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            2618..3433
                     /gene="metQ"
                     /locus_tag="ACHOUK_000107"
     CDS             2618..3433
                     /gene="metQ"
                     /locus_tag="ACHOUK_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFP9895639.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKNGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            3551..3955
                     /gene="rcsF"
                     /locus_tag="ACHOUK_000108"
     CDS             3551..3955
                     /gene="rcsF"
                     /locus_tag="ACHOUK_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFP9895640.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQALCIGSALNITAK"
     gene            3952..4659
                     /gene="tsaA"
                     /locus_tag="ACHOUK_000109"
     CDS             3952..4659
                     /gene="tsaA"
                     /locus_tag="ACHOUK_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFP9895641.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDCVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            4770..6488
                     /gene="proS"
                     /locus_tag="ACHOUK_000110"
     CDS             4770..6488
                     /gene="proS"
                     /locus_tag="ACHOUK_000110"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFP9895642.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIKK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFTAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            6541..7365
                     /gene="yaeF"
                     /locus_tag="ACHOUK_000111"
     CDS             6541..7365
                     /gene="yaeF"
                     /locus_tag="ACHOUK_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFP9895643.1"
                     /translation="MDKPKAYCRLLLPCFLLLSACTVDISQPDSSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITVPDLKPGDLLFSSSLEVTSFGIRVFSTSSVSHVAIYLGENN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATEITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVVEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTR"
     gene            complement(7565..8275)
                     /gene="nlpE"
                     /locus_tag="ACHOUK_000112"
                     /gene_synonym="cutF"
     CDS             complement(7565..8275)
                     /gene="nlpE"
                     /locus_tag="ACHOUK_000112"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFP9895644.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGQ"
     gene            complement(8289..8699)
                     /gene="arfB"
                     /locus_tag="ACHOUK_000113"
     CDS             complement(8289..8699)
                     /gene="arfB"
                     /locus_tag="ACHOUK_000113"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFP9895645.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRSGRE"
     gene            complement(8696..9241)
                     /gene="yaeQ"
                     /locus_tag="ACHOUK_000114"
     CDS             complement(8696..9241)
                     /gene="yaeQ"
                     /locus_tag="ACHOUK_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFP9895646.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCAQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            9407..9607
                     /gene="yaeP"
                     /locus_tag="ACHOUK_000115"
     CDS             9407..9607
                     /gene="yaeP"
                     /locus_tag="ACHOUK_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFP9895647.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            9594..9854
                     /gene="rof"
                     /locus_tag="ACHOUK_000116"
     CDS             9594..9854
                     /gene="rof"
                     /locus_tag="ACHOUK_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFP9895648.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(9907..11202)
                     /gene="tilS"
                     /locus_tag="ACHOUK_000117"
     CDS             complement(9907..11202)
                     /gene="tilS"
                     /locus_tag="ACHOUK_000117"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFP9895649.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGGAL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAETTARSAALCAE
                     QESLLDELLADDLAHCQSPQGTLQIVPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSENIVPWQTWLQPLELPA
                     GQGSVQLNAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGGFVTQEGVAEGENGISFVWQRNA"
     gene            complement(11267..11656)
                     /gene="yaeR"
                     /locus_tag="ACHOUK_000118"
     CDS             complement(11267..11656)
                     /gene="yaeR"
                     /locus_tag="ACHOUK_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9895650.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            complement(11713..13854)
                     /gene="ldcC"
                     /locus_tag="ACHOUK_000119"
     CDS             complement(11713..13854)
                     /gene="ldcC"
                     /locus_tag="ACHOUK_000119"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFP9895651.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALCF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            complement(13953..14912)
                     /gene="accA"
                     /locus_tag="ACHOUK_000120"
     CDS             complement(13953..14912)
                     /gene="accA"
                     /locus_tag="ACHOUK_000120"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFP9895652.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            complement(14925..18407)
                     /gene="dnaE"
                     /locus_tag="ACHOUK_000121"
     CDS             complement(14925..18407)
                     /gene="dnaE"
                     /locus_tag="ACHOUK_000121"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFP9895653.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQHLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            complement(18444..19040)
                     /gene="rnhB"
                     /locus_tag="ACHOUK_000122"
     CDS             complement(18444..19040)
                     /gene="rnhB"
                     /locus_tag="ACHOUK_000122"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFP9895654.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            complement(19037..20185)
                     /gene="lpxB"
                     /locus_tag="ACHOUK_000123"
     CDS             complement(19037..20185)
                     /gene="lpxB"
                     /locus_tag="ACHOUK_000123"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFP9895655.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NIILEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNSARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAAVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            complement(20185..20973)
                     /gene="lpxA"
                     /locus_tag="ACHOUK_000124"
     CDS             complement(20185..20973)
                     /gene="lpxA"
                     /locus_tag="ACHOUK_000124"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFP9895656.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMAAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            complement(20977..21432)
                     /gene="fabZ"
                     /locus_tag="ACHOUK_000125"
     CDS             complement(20977..21432)
                     /gene="fabZ"
                     /locus_tag="ACHOUK_000125"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFP9895657.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            complement(21537..22562)
                     /gene="lpxD"
                     /locus_tag="ACHOUK_000126"
     CDS             complement(21537..22562)
                     /gene="lpxD"
                     /locus_tag="ACHOUK_000126"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFP9895658.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            complement(22566..23051)
                     /gene="skp"
                     /locus_tag="ACHOUK_000127"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             complement(22566..23051)
                     /gene="skp"
                     /locus_tag="ACHOUK_000127"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFP9895659.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            complement(23172..25604)
                     /gene="bamA"
                     /locus_tag="ACHOUK_000128"
     CDS             complement(23172..25604)
                     /gene="bamA"
                     /locus_tag="ACHOUK_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFP9895660.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            complement(25634..26986)
                     /gene="rseP"
                     /locus_tag="ACHOUK_000129"
     CDS             complement(25634..26986)
                     /gene="rseP"
                     /locus_tag="ACHOUK_000129"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFP9895661.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            complement(26998..27855)
                     /gene="cdsA"
                     /locus_tag="ACHOUK_000130"
     CDS             complement(26998..27855)
                     /gene="cdsA"
                     /locus_tag="ACHOUK_000130"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFP9895662.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            complement(27868..28626)
                     /gene="ispU"
                     /locus_tag="ACHOUK_000131"
                     /gene_synonym="uppS"
     CDS             complement(27868..28626)
                     /gene="ispU"
                     /locus_tag="ACHOUK_000131"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFP9895663.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            complement(28815..30011)
                     /gene="ispC"
                     /locus_tag="ACHOUK_000132"
     CDS             complement(28815..30011)
                     /gene="ispC"
                     /locus_tag="ACHOUK_000132"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFP9895664.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDASAREVARKE
                     VMRLAS"
     gene            complement(30103..30660)
                     /gene="frr"
                     /locus_tag="ACHOUK_000133"
     CDS             complement(30103..30660)
                     /gene="frr"
                     /locus_tag="ACHOUK_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFP9895665.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            complement(30810..31535)
                     /gene="pyrH"
                     /locus_tag="ACHOUK_000134"
     CDS             complement(30810..31535)
                     /gene="pyrH"
                     /locus_tag="ACHOUK_000134"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFP9895666.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            complement(31682..32533)
                     /gene="tsf"
                     /locus_tag="ACHOUK_000135"
     CDS             complement(31682..32533)
                     /gene="tsf"
                     /locus_tag="ACHOUK_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFP9895667.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            complement(32791..33516)
                     /gene="rpsB"
                     /locus_tag="ACHOUK_000136"
     CDS             complement(32791..33516)
                     /gene="rpsB"
                     /locus_tag="ACHOUK_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFP9895668.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            33884..34678
                     /gene="map"
                     /locus_tag="ACHOUK_000137"
     CDS             33884..34678
                     /gene="map"
                     /locus_tag="ACHOUK_000137"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFP9895669.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            34740..37412
                     /gene="glnD"
                     /locus_tag="ACHOUK_000138"
     CDS             34740..37412
                     /gene="glnD"
                     /locus_tag="ACHOUK_000138"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFP9895670.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVNVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEVLHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            37443..38267
                     /gene="dapD"
                     /locus_tag="ACHOUK_000139"
     CDS             37443..38267
                     /gene="dapD"
                     /locus_tag="ACHOUK_000139"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFP9895671.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGDIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            38582..38968
                     /gene="yaeH"
                     /locus_tag="ACHOUK_000140"
     CDS             38582..38968
                     /gene="yaeH"
                     /locus_tag="ACHOUK_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFP9895672.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(39057..40214)
                     /gene="cdaR"
                     /locus_tag="ACHOUK_000141"
     CDS             complement(39057..40214)
                     /gene="cdaR"
                     /locus_tag="ACHOUK_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFP9895673.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDVEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(40369..41793)
                     /gene="degP"
                     /locus_tag="ACHOUK_000142"
     CDS             complement(40369..41793)
                     /gene="degP"
                     /locus_tag="ACHOUK_000142"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFP9895674.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            complement(41923..43440)
                     /gene="dgt"
                     /locus_tag="ACHOUK_000143"
     CDS             complement(41923..43440)
                     /gene="dgt"
                     /locus_tag="ACHOUK_000143"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFP9895675.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAVL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRSPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            43524..44222
                     /gene="mtnN"
                     /locus_tag="ACHOUK_000144"
     CDS             43524..44222
                     /gene="mtnN"
                     /locus_tag="ACHOUK_000144"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFP9895676.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            44215..45015
                     /gene="btuF"
                     /locus_tag="ACHOUK_000145"
     CDS             44215..45015
                     /gene="btuF"
                     /locus_tag="ACHOUK_000145"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFP9895677.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATNIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            45053..45676
                     /gene="yadS"
                     /locus_tag="ACHOUK_000146"
     CDS             45053..45676
                     /gene="yadS"
                     /locus_tag="ACHOUK_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFP9895678.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRHLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(45723..46067)
                     /gene="erpA"
                     /locus_tag="ACHOUK_000147"
     CDS             complement(45723..46067)
                     /gene="erpA"
                     /locus_tag="ACHOUK_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFP9895679.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(46060..46125)
                     /gene="yadW"
                     /locus_tag="ACHOUK_000148"
     CDS             complement(46060..46125)
                     /gene="yadW"
                     /locus_tag="ACHOUK_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFP9895680.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            complement(46149..47570)
                     /gene="clcA"
                     /locus_tag="ACHOUK_000149"
     CDS             complement(46149..47570)
                     /gene="clcA"
                     /locus_tag="ACHOUK_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFP9895681.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDVFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            complement(47582..47629)
                     /gene="yadX"
                     /locus_tag="ACHOUK_000150"
     CDS             complement(47582..47629)
                     /gene="yadX"
                     /locus_tag="ACHOUK_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFP9895682.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            47795..49075
                     /gene="hemL"
                     /locus_tag="ACHOUK_000151"
     CDS             47795..49075
                     /gene="hemL"
                     /locus_tag="ACHOUK_000151"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFP9895683.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPD
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            complement(49110..51092)
                     /gene="fhuB"
                     /locus_tag="ACHOUK_000152"
     CDS             complement(49110..51092)
                     /gene="fhuB"
                     /locus_tag="ACHOUK_000152"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFP9895684.1"
                     /translation="MSKRIALFPALLLALLVIAATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGGALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(51089..51979)
                     /gene="fhuD"
                     /locus_tag="ACHOUK_000153"
     CDS             complement(51089..51979)
                     /gene="fhuD"
                     /locus_tag="ACHOUK_000153"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFP9895685.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPESVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMNPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            complement(51979..52776)
                     /gene="fhuC"
                     /locus_tag="ACHOUK_000154"
     CDS             complement(51979..52776)
                     /gene="fhuC"
                     /locus_tag="ACHOUK_000154"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFP9895686.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            complement(52827..55070)
                     /gene="fhuA"
                     /locus_tag="ACHOUK_000155"
     CDS             complement(52827..55070)
                     /gene="fhuA"
                     /locus_tag="ACHOUK_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFP9895687.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLDLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            complement(55290..57824)
                     /gene="mrcB"
                     /locus_tag="ACHOUK_000156"
     CDS             complement(55290..57824)
                     /gene="mrcB"
                     /locus_tag="ACHOUK_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFP9895688.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            complement(58020..60449)
                     /gene="hrpB"
                     /locus_tag="ACHOUK_000157"
     CDS             complement(58020..60449)
                     /gene="hrpB"
                     /locus_tag="ACHOUK_000157"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFP9895689.1"
                     /translation="MSSLPVAAVLPELLTALDYAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPTHQRFDDAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRISQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERATAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAGKWLPEYDWPAVDDESLLATLETWLLPHMAGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            60523..61053
                     /gene="thpR"
                     /locus_tag="ACHOUK_000158"
     CDS             60523..61053
                     /gene="thpR"
                     /locus_tag="ACHOUK_000158"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFP9895690.1"
                     /translation="MSDPQRLFFAIDLPAEIREQIIHWRATHFPLEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWVLTQ"
     gene            61068..61772
                     /gene="sfsA"
                     /locus_tag="ACHOUK_000159"
     CDS             61068..61772
                     /gene="sfsA"
                     /locus_tag="ACHOUK_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFP9895691.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            61950..62405
                     /gene="dksA"
                     /locus_tag="ACHOUK_000160"
     CDS             61950..62405
                     /gene="dksA"
                     /locus_tag="ACHOUK_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFP9895692.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            62472..63368
                     /gene="gluQRS"
                     /locus_tag="ACHOUK_000161"
     CDS             62472..63368
                     /gene="gluQRS"
                     /locus_tag="ACHOUK_000161"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFP9895693.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARHGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            63428..64825
                     /gene="pcnB"
                     /locus_tag="ACHOUK_000162"
     CDS             63428..64825
                     /gene="pcnB"
                     /locus_tag="ACHOUK_000162"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFP9895694.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            64822..65304
                     /gene="folK"
                     /locus_tag="ACHOUK_000163"
     CDS             64822..65304
                     /gene="folK"
                     /locus_tag="ACHOUK_000163"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFP9895695.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILTVSSFYRTP
                     PLGPQDQPDYLNAAVALETTLAPEELLNHTQQIELQQGRVRKAERWGPRTLDLDIMLF
                     GNETINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGETLREILTSLAVLQPAIWY
                     "
     gene            65585..66181
                     /locus_tag="ACHOUK_000164"
     CDS             65585..66181
                     /locus_tag="ACHOUK_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9895696.1"
                     /translation="MKKALLAAALVMASGSALAVDGGHIDFNGMVQSGTCKVGVVDTG
                     MHSVTTDGVVTLDTANVTDTFAEVSATAVGLLPKEFMISVECDPGAPKNAELTMGSAS
                     YANTSGTLNNNMNITVNGIAPAQNVNIAVHNMKNKAGAAEIKQVHMNNSSEVQELTLD
                     AEGKGQYVFNASYVKAPNSPAVTAGHVTTNALYTVAYK"
     gene            66268..66993
                     /locus_tag="ACHOUK_000165"
     CDS             66268..66993
                     /locus_tag="ACHOUK_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFP9895697.1"
                     /translation="MFPLVKKTVSALFVSTLLASAPAFADIIISGTRIIYNADKKDVN
                     VRLENKGNRPLLIQNWLDTGDDNADPSQIKVPFTATPPVSRVEPKRGQTVKVMYTGAT
                     ALPKDRESVYWFNVLEVPPKPKDAEADKNLLQLAFRTRIKLFYRPSGLQGEPAEAPAK
                     ITWKLNNAQLQANNPTPYYVSFNEVKLESGGKTYNVNSSMVTPFSQASFGVTSLPGSV
                     SSGKVVFKAINDFGGNIDGSATF"
     gene            67035..69635
                     /locus_tag="ACHOUK_000166"
     CDS             67035..69635
                     /locus_tag="ACHOUK_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HFP9895698.1"
                     /translation="MYQFTHQKSRIPKKTLLAACCALFYSSNGAAADTVEYDSSFLMG
                     TGASTIDVKRYAQGNPTPPGLYNVRVFVNGQATSSLEIPFVDIGENSAAACLTHKNLA
                     QLHIKQPEQPVTLLAREGEEEDCLDLAKSYEKADVCFDGSDQFLDLTIPQAYVLKSYG
                     GYVDPSLWESGINAATLAYTLNAYHTSSDNDNSDSVYGAFNSGINLGAWHFRARGNYN
                     WTTDNGSDFDFQDRYLQRDIPAIRSQIIMGDAYTTGETFDSVNVRGVRLYSDSRMLPS
                     ALASYAPTIRGVANSNAKVTVTQSGYKIYETTVPPGEFVIDDISPSGFGSELVVTIEE
                     ADGSKRTFTQPFSSVVQMQRPGVGRWDFSAGKVIDDSLRSEPNMGQASYYYGLNNLFT
                     GYTGIQFTDNNYLAGLLGVGINTSIGAFAVDVTHSRAEIPDDKTYQGQSYRVTWNKLF
                     QDTGTSFNLAAYRYSTQDYLGLHDALVLIDDAKHLSADEDKNTMQTYSRMKNQFTVSI
                     NQPLNIAYEDYGSLFISGSWTYYWAANNSRTEYNVGYSKSVSWGSFSVNLQRSWNEDG
                     EKDDAMYVSVSVPIENILGGKRKSSGFRNLNTQLNTDFDGSHQLNVNSSGNTENNLVN
                     YSVNAGYSLDKNAGDLASVGGYLNYESGLGGISASASATSDNSQQYSISTDGGFVLHS
                     GGLTFTNNSFSSNDTLVLINALGAKGARINNSNNEIDRWGYAVTSSVSPYRENRVGLN
                     IETLENDVELKSTSATTVPRSGSVVLTRFETDEGRSAVLNITAANGKSIPFAAEVYQG
                     EVMIGSMGQGGQAFVRGINDSGELIVRWYENNQTIDCKLHYQFPAQPQTQGSTNTLLL
                     NNLTCQVANH"
     gene            69649..70197
                     /locus_tag="ACHOUK_000167"
     CDS             69649..70197
                     /locus_tag="ACHOUK_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9895699.1"
                     /translation="MKRILLTSALIGLGLPAVGSATDLNVDFTATVLATTCTITIVED
                     GGPAVTGSNDEYSLTIPDVGLDKVATAAPEAQANFKLKASDCSNGYSKIFTTLTGTTV
                     SGKLIVNEATSGAAGVGMGIKRRDTADSTFFTPNNTDKFEWSADEKASGVPLTVALRE
                     TTAGAGRTGAFQAKATFNFTYE"
     gene            70212..70817
                     /locus_tag="ACHOUK_000168"
     CDS             70212..70817
                     /locus_tag="ACHOUK_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9895700.1"
                     /translation="MKLKVIATLIATVAVGVSFNSNFASASTTSASLTVNSNLTMGTC
                     SAQIMDNSNKVINEVVFGNVYISELGAKSKVQQFKIRFSNCSGLPQNSAQIVLAPNGI
                     SCAGSQSSSAGFSNKFTDASAATRTAVEVWTTDTPESNGSTQFHCAQKIPVPVTLPAD
                     TTTQPYDYPLSARMTVAEGRLVTDVRPGNFRSPTTFTITYQ"
     gene            70838..71428
                     /locus_tag="ACHOUK_000169"
     CDS             70838..71428
                     /locus_tag="ACHOUK_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFP9895701.1"
                     /translation="MLCRHHKIVHFLGLATALITPFAYSGQDVDLTAKIVPSTCQVEV
                     SNNGVVDLGTVTLDYFADNVTPTTDYAGGKTFNVNVVSCDNIQTTQSQMKLDFQPQAG
                     SLAQANNQIFSNEYEQQATGAKNVGIVIFSAQPNQQTFNVRGTDGSSTAIYSVAPGNA
                     VPSTWTFYSRMQRVNNALPPESGMVRSQVIVNVSYE"
     gene            71440..72549
                     /locus_tag="ACHOUK_000170"
     CDS             71440..72549
                     /locus_tag="ACHOUK_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9895702.1"
                     /translation="MQRKGNKLLIQLCSVILLFFTTSWYALANECYIERNAEGDYHMK
                     ISSTQLSLASQMVEVPTEIAEATWDVNIQLRGDAIGCKSLGDSKAVHFLNTADPSLIS
                     TYTTTNGAALLKTTVPGIVYSVELLCLSCGAADELDLWLPAQSGADNFIPSTQTKWAY
                     EYSDQSWYLRFRLFITPEFKPKNGVSSGTTIAGKIASWYIGTNDQPWINFYIDNDSLK
                     FFVDEPTCATVALAQDQGNVSGNQVTLGNSYVSEVKNGLTREIPFSIRAEYCYASKIT
                     VKLKAANKPSDATLVGKTTGSASGVAVKVNSTYDNSKVLLKADGSNTVDYNFAAWSNN
                     LLFLPFTAQLVPDGSGNAVGVGTFSGNATFSFTYE"
     gene            72684..73478
                     /gene="panB"
                     /locus_tag="ACHOUK_000171"
     CDS             72684..73478
                     /gene="panB"
                     /locus_tag="ACHOUK_000171"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFP9895703.1"
                     /translation="MKPTTIASLQKCKQEKKRFATITAYDYSFAKLFAEEGLNVMLVG
                     DSLGMTVQGHESTLPVTVEDIAYHTTAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            73490..74341
                     /gene="panC"
                     /locus_tag="ACHOUK_000172"
     CDS             73490..74341
                     /gene="panC"
                     /locus_tag="ACHOUK_000172"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFP9895704.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(74640..75536)
                     /gene="rpnC"
                     /locus_tag="ACHOUK_000173"
     CDS             complement(74640..75536)
                     /gene="rpnC"
                     /locus_tag="ACHOUK_000173"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFP9895705.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSESQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            75810..76190
                     /gene="panD"
                     /locus_tag="ACHOUK_000174"
     CDS             75810..76190
                     /gene="panD"
                     /locus_tag="ACHOUK_000174"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFP9895706.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(76194..77423)
                     /gene="yadE"
                     /locus_tag="ACHOUK_000175"
     CDS             complement(76194..77423)
                     /gene="yadE"
                     /locus_tag="ACHOUK_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFP9895707.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFDKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMVQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     EPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(77487..77927)
                     /gene="yadI"
                     /locus_tag="ACHOUK_000176"
     CDS             complement(77487..77927)
                     /gene="yadI"
                     /locus_tag="ACHOUK_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9895708.1"
                     /translation="MLGWVITCHDDRAQEILDVLEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGAPEVSSIWHQQQKNPPFVLKHNLYEY"
     gene            complement(78032..78802)
                     /gene="yadH"
                     /locus_tag="ACHOUK_000177"
     CDS             complement(78032..78802)
                     /gene="yadH"
                     /locus_tag="ACHOUK_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9895709.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYPLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(78799..79725)
                     /gene="yadG"
                     /locus_tag="ACHOUK_000178"
     CDS             complement(78799..79725)
                     /gene="yadG"
                     /locus_tag="ACHOUK_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9895710.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            79834..80496
                     /gene="can"
                     /locus_tag="ACHOUK_000179"
     CDS             79834..80496
                     /gene="can"
                     /locus_tag="ACHOUK_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFP9895711.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLDEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(80537..81073)
                     /gene="hpt"
                     /locus_tag="ACHOUK_000180"
     CDS             complement(80537..81073)
                     /gene="hpt"
                     /locus_tag="ACHOUK_000180"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFP9895712.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            81279..83669
                     /gene="gcd"
                     /locus_tag="ACHOUK_000181"
     CDS             81279..83669
                     /gene="gcd"
                     /locus_tag="ACHOUK_000181"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFP9895713.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(83871..85421)
                     /gene="cueO"
                     /locus_tag="ACHOUK_000182"
     CDS             complement(83871..85421)
                     /gene="cueO"
                     /locus_tag="ACHOUK_000182"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFP9895714.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKERAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            85587..85934
                     /gene="yacC"
                     /locus_tag="ACHOUK_000183"
     CDS             85587..85934
                     /gene="yacC"
                     /locus_tag="ACHOUK_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFP9895715.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            86040..86906
                     /gene="speE"
                     /locus_tag="ACHOUK_000184"
     CDS             86040..86906
                     /gene="speE"
                     /locus_tag="ACHOUK_000184"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFP9895716.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAVHTAAFALP
                     QYLQDALASQPS"
     gene            86922..87716
                     /gene="speD"
                     /locus_tag="ACHOUK_000185"
     CDS             86922..87716
                     /gene="speD"
                     /locus_tag="ACHOUK_000185"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFP9895717.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(87754..88116)
                     /gene="yacL"
                     /locus_tag="ACHOUK_000186"
     CDS             complement(87754..88116)
                     /gene="yacL"
                     /locus_tag="ACHOUK_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFP9895718.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(88292..90889)
                     /gene="acnB"
                     /locus_tag="ACHOUK_000187"
     CDS             complement(88292..90889)
                     /gene="acnB"
                     /locus_tag="ACHOUK_000187"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFP9895719.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAVAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            91244..93097
                     /gene="yacH"
                     /locus_tag="ACHOUK_000188"
     CDS             91244..93097
                     /gene="yacH"
                     /locus_tag="ACHOUK_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFP9895720.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVETQSTQ
                     LAVSDAAAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PTNVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKTVPVTQTVTAPVIPSN
                     TVSTANPVITEPATTVISIEPGNPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDNYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVHEKMQTNPQNQQRREAARERIQPASPEQRQ
                     AVREKMQTNPQIQQRRDAARERIQSASPEQRQVFKEKVQQRPLNQQQRDNARQRVQSA
                     SPEQRQVFREKVQESRPQRLNDSNHTARLNNEQRSAVRERLSERGARRLER"
     gene            complement(93168..94592)
                     /gene="lpdA"
                     /locus_tag="ACHOUK_000189"
     CDS             complement(93168..94592)
                     /gene="lpdA"
                     /locus_tag="ACHOUK_000189"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFP9895721.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(94800..96692)
                     /gene="aceF"
                     /locus_tag="ACHOUK_000190"
     CDS             complement(94800..96692)
                     /gene="aceF"
                     /locus_tag="ACHOUK_000190"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFP9895722.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAVPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNTGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(96707..99370)
                     /gene="aceE"
                     /locus_tag="ACHOUK_000191"
     CDS             complement(96707..99370)
                     /gene="aceE"
                     /locus_tag="ACHOUK_000191"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFP9895723.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(99531..100295)
                     /gene="pdhR"
                     /locus_tag="ACHOUK_000192"
     CDS             complement(99531..100295)
                     /gene="pdhR"
                     /locus_tag="ACHOUK_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFP9895724.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT        24326 a        27308 c        24833 g        23967 t
ORIGIN      
        1 attttttctc ttcaaccgaa ccggctgttt gtgtgaagtg attcacattc gccgtgtcga
       61 tggaggcgca ttatagggag tcggctcagg aagacaagcg gaaaaatgca tttttatttc
      121 aaccgctcat cttttaatca ttgcgccggt ttttgctgct tttttatcgc ttgcggcagg
      181 tctgccaggc tatttaacac ccaatctgcc gcgttttctg cttcaggtgt aataggttta
      241 cccgtacgca ccagcacttt tgttcccacg ttcgccgcaa ccgctgcctg catatcttct
      301 aatttatcgc ccaccatata agaagcggcc atatcaatat gcaaataatc gcgtgctgac
      361 aaaagcatcc ccggatgtgg tttgcggcaa tcgcagacct ggcgaaactc ttcaacacta
      421 ccctgcggat gatgcgggca ataatagata ccatccagat cgacatctcg gtccgccagc
      481 gaccagtcca tccactcggt cagcgtttca aactgtgctt cggtaaattt accgcgagca
      541 atgccagact ggttggtgac taccaccagc gcaaagccca ttttttttag ctcgcgcatg
      601 gcgtcaataa caccgtcgat aaattcaaag ttgtcgatct catggacata gccgtgatcg
      661 acattaatgg tgccatcacg gtcaagaaaa attgcgggta cgctcttcgc caccttttat
      721 agctccttaa taaggcatgt gacgctagta tcgcatgttt cgacctgcaa gaaagtgctc
      781 ttcgcataaa cctgattgat ttagacgtct ggatgcctta acatccattt cattgacggc
      841 gttgcccgtt tcaggcattc gagatgccac gactaactta atgacgataa taaataatca
      901 atgataaaac tttcgaatat caccaaagtg ttccaccagg gcacccgcac catccaggcg
      961 ttgaacaacg tcagcctgca tgtgccagct ggacaaattt atggcgttat cggtgcctca
     1021 ggcgcgggta agagtacgct tatacgttgt gtaaacctgc tggagcgccc aaccgagggt
     1081 agcgtgctgg tcgatggaca ggaactgacc acgctgtcag aatccgagtt gaccaaagct
     1141 cgccgccaga ttggtatgat tttccagcat tttaacctgc tctcttcgcg tactgttttt
     1201 ggcaacgtgg ctctgccgct ggagctggac aatacaccga aagacgagat caaacgtcgc
     1261 gtgacggaat tgctgtcatt ggttggtctt ggagataagc atgatagcta cccgtcgaat
     1321 ctttccggtg ggcagaaaca acgtgtggcg attgcccgtg cattagccag caatcccaaa
     1381 gtattgctgt gtgatgaagc caccagcgcg ctagacccgg caacgacacg ttctattctc
     1441 gaactgctga aagacattaa ccgccgtctg ggtttgacga ttctgttgat cacccacgaa
     1501 atggacgttg tgaagcgtat ttgtgattgc gttgcggtca tcagcaatgg cgaactgatc
     1561 gagcaggaca cggtaagtga agtgttctca catccgaaaa cgccgctggc gcagaagttt
     1621 attcagtcaa ccctgcatct ggatatcccg gaagattacc aggaacgtct gcaagcggag
     1681 ccatttactg actgcgtgcc gatgctgcgt ctggagttta ccggtcaatc ggtcgatgcc
     1741 ccactgcttt ctgaaaccgc gcgtcgtttc aacgtcaaca acaacattat tagcgcgcag
     1801 atggattacg ccggtggcgt taagttcggc atcatgctga ctgaaatgca cggcacacaa
     1861 caagatacgc aagccgccat tgcctggctg caggaacacc atgtaaaagt agaggtactg
     1921 ggttatgtct gagccgatga tgtggctgct ggttcgtggc gtatgggaaa cgctggcaat
     1981 gaccttcgta tccggatttt ttgggtttgt ggttggtctg ccggttggcg tcctgcttta
     2041 tgtcacgcgt ccggggcaaa ttattgctaa cgcgaagctg tatcgtaccg tttctgcgat
     2101 tgtgaacatt ttccgttcca tcccgttcat tatcttgctg gtatggatga ttccgtttac
     2161 ccgcgttatt gtcggtacat cgattggttt gcaggcagca attgttccgt taaccgttgg
     2221 tgcagcaccg tttattgccc gtatggtcga gaacgctctg ctggagatcc caaccgggtt
     2281 aattgaagct tcccgcgcaa tgggtgccac gccaatgcag atcgtccgta aagtgctgtt
     2341 accggaagcg ttgccgggtc tggtgaatgc ggcaactatc accctgatta ccctggtcgg
     2401 ttattccgcg atgggtggtg cagtcggtgc cggtggttta ggtcagattg gctatcagta
     2461 tggctacatc ggctataacg cgacggtgat gaatacggta ctggtattgc tggtcattct
     2521 ggtttattta attcagttcg caggcgaccg catcgtccgg gctgtcactc gcaagtaacg
     2581 ttcaacacaa cataaataat tgaagaagga ataaggtatg gcgttcaaat tcaaaacctt
     2641 tgcggcagtg ggagccctga ttggatcact ggcactggta ggctgcggtc aggatgaaaa
     2701 agatccaaac cacattaaag tcggcgtgat tgttggtgcc gaacagcagg ttgcagaagt
     2761 cgcgcagaaa gttgcgaaag acaaatatgg cctggacgtt gagctggtaa ccttcaacga
     2821 ctatgttctg ccaaacgaag cattgagcaa aggcgatatc gacgccaacg ccttccagca
     2881 taaaccgtac cttgatcagc aactgaaaga tcgtggctac aaactggtcg cagtaggcaa
     2941 cacttttgtt tatccgattg ctggttactc caagaaaatc aaatcactgg atgaactgca
     3001 ggatggttcg caggttgccg tgccaaacga tccaactaac cttggtcgtt cactgctgct
     3061 gctgcaaaaa gtgggcttga tcaaactgaa agatggcgtt ggcctgctgc cgaccgttct
     3121 tgatgttgtt gagaacccaa aaaatctgaa aattgttgaa ctggaagcac cgcaactgcc
     3181 gcgttctctg gacgacgcgc aaatcgctct ggcagttatt aacaccacct atgccagcca
     3241 gattggcctg actccagcga aaaacggtat ctttgtcgaa gataaagact ccccgtacgt
     3301 aaacctgatc gtaacgcgtg aagataacaa agacgccgag aacgtgaaga aattcgtcca
     3361 ggcttatcag tctgacgaag tttacgaagc agcaaacaaa gtgtttaacg gcggcgctgt
     3421 taaaggctgg taatttttag gctgtttcca caatttgtaa tatcattcag gacgggcgct
     3481 tgcccgtctt gtcattttta caagctcctg attcaatatt gacgttttga tcatacattg
     3541 aggaaatact atgcgtgctt taccgatctg tttagtagca ctcatgctaa gcggctgttc
     3601 catgttaagc agatcccctg tcgaacccgt tcaaagcact gcaccccagc cgaaagcgga
     3661 gcctgcaaaa ccgaaagcgc cgcgcgccac gccggtccga atttatacca atgcagaaga
     3721 attagtcggc aaaccgttcc gcgatctcgg tgaagtcagt ggcgactctt gccaggcctc
     3781 taatcaggac tctccgccga gcattccaac cgcgcgtaag cggatgcaaa tcaacgcctc
     3841 gaaaatgaaa gccaatgcgg tattactgca tagctgcgaa gttaccagcg gtacgccagg
     3901 ctgctatcgt caggctttat gtatcggttc tgcgcttaac attacggcga aatgagcagt
     3961 ttccagttcg agcaaatagg tgttattcgc tcgccctata aagaaaaatt cgccgttccg
     4021 cgccagccag gtctggtaaa aagcgccaac ggcgaactac atctcattgc tccctacaac
     4081 caggctgacg ccgttcgcgg cctggaagca ttcagccatt tatggatcct tttcgtcttt
     4141 catcaaacga tggaaggcgg ctggcgtccg actgtgcgtc cgcctcgtct tggcggtaat
     4201 gccagaatgg gtgttttcgc tacgcgctcc actttccgcc ccaacccgat tggtatgtcg
     4261 ctggtagagc tgaaagaggt tgtttgccat aaagactgcg tgattctgaa gctcggcagc
     4321 ctggatctgg tcgatggtac gccggtagtg gatatcaaac cgtatctccc ctttgccgaa
     4381 tcgcttcccg atgccagtgc cagctatgcg caaagtgcgc cagctgcaga gatggcggtg
     4441 agttttaccg cagaggtcga aaagcagctt ttgacgctgg agaagcgtta tccgcagtta
     4501 acgctgttta tccgcgaagt actggcgcag gacccgcgcc cggcctatcg taaaggtgag
     4561 gaaacgggca aaacctatgc cgtctggcta catgatttta acgttcgctg gcgcgtcacc
     4621 gacgcaggtt ttgaagtctt tgcgctggaa ccgcgttaaa ttcacgccct tctcttttga
     4681 catttctttt gcactggtaa actaaatcac tttttttgtc ccaggctcgc cttgagcctg
     4741 ttcgaccttc caactggaac cgtaacaaca tgcgtactag ccaatacctg ctctccactc
     4801 tcaaggagac acctgccgac gccgaggtga tcagccatca gctgatgctg cgcgccggga
     4861 tgatccgcaa gctggcctcc gggttatata cctggctgcc gaccggcgtg cgcgttctga
     4921 aaaaagtcga aaacatcgtg cgtgaagaga tgaacaacgc cggtgcaatc gaggtgtcga
     4981 tgccggtggt tcagccagcc gatttgtggc aagagagtgg tcgttgggaa cagtacggtc
     5041 cggaactgct gcgttttgtt gaccgtggcg agcgtccgtt cgtactcggc ccaactcatg
     5101 aagaagttat cactgacctg attcgtaacg agcttagctc ttacaaacag ctgccgctga
     5161 acttctatca gatccagacc aagttccgcg acgaagtgcg tccgcgtttc ggcgtcatgc
     5221 gttcccgcga attcctgatg aaagatgctt actctttcca tacttctcag gaatccctgc
     5281 aggaaaccta cgatgcaatg tatgcggcct acagcaaaat cttcagccgc atggggctgg
     5341 atttccgcgc cgtacaggcc gacaccggtt ctatcggcgg cagcgcctct cacgaattcc
     5401 aggtgctggc gcagagcggt gaagacgatg tggtcttctc cgacacctct gactatgcag
     5461 cgaacattga gctggcagaa gctatcgcgc cgaaagaacc gcgcgctgct gctacccagg
     5521 aaatgacgct ggttgatacg ccgaacgcga aaaccatcgc ggaactggtt gaacagttca
     5581 atctgccgat taagaaaacg gttaagactc tgctggttaa agcggttgaa ggcagtagct
     5641 tcccgctggt tgcgctgctg gtgcgcggtg atcacgagct gaacgaagtt aaagcagaaa
     5701 aactgccgca ggttgccagc ccgctgactt tcgcgaccga agaagaaatt cgtgccgtgg
     5761 ttaaagccgg tccgggttca ctgggtccgg taaacatgcc gattccggtg gtgattgacc
     5821 gtaccgttgc ggcgatgagt gatttcactg ctggtgctaa catcgatggt aaacactact
     5881 tcggcatcaa ctgggatcgc gatgtcgcta ccccggaagt tgcggatatc cgtaacgtgg
     5941 tggctggcga tccaagcccg gatggccagg gtacgctgct gatcaaacgt ggtatcgaag
     6001 ttggtcacat cttccagctg ggtaccaagt actccgaagc actgaaagcc tccgtacagg
     6061 gtgaagatgg ccgtaaccaa atcctgacta tgggttgcta cggtatcggg gtaacgcgcg
     6121 tagtggctgc ggcgattgag cagaactacg acgagcgcgg catcgtatgg cctgacgcta
     6181 tcgcgccgtt ccaggtggca attctgccga tgaacatgca caaatccttc cgcgtacaag
     6241 agcttgctga gaaactgtac agcgaactgc gtgcacaagg tatcgaagtg ctgctggatg
     6301 accgaaaaga acgtccgggc gtgatgtttg ccgatatgga actgatcggt attccgcaca
     6361 ctattgtgct gggcgaccgt aacctcgaca acgacgatat cgaatataaa tatcgtcgca
     6421 acggcgagaa acagttaatt aagactggtg acatcgtcga atatctggtg aaacagatta
     6481 aaggctgatg ccagaaaggg tcctgaattt cagggccctt ttttacatgg attgtataaa
     6541 atggataaac caaaggcgta ctgccgcctg cttctcccct gctttctttt actttcggcc
     6601 tgtaccgttg atatcagcca gccagactca tcagccacag cagtagatgc cgaggcgaaa
     6661 acatgggctg tcaaattcca gcatcaaagc tctttcaccg aacaatcaat aaaagaaata
     6721 acggtacctg atctcaaacc cggcgatttg ctgttctcct caagcctgga ggtaacctca
     6781 tttgggatcc gcgttttcag cacttcctct gtgagtcacg ttgcaatcta tctgggtgaa
     6841 aataacgttg cagaagcgac aggcgctggc gtccagattg tttcccttaa aaaagcgatg
     6901 aagcatagtg ataaactttt cgtcttacga gtcccggatc ttaccccgca acaagccaca
     6961 gaaatcaccg cgttcgccaa taaaatcaaa gatagcggtt ataactatcg cggcattgtc
     7021 gaatttattc cctttatggt gactcgccag atgtgctcac tgaatccgtt ctccgaggat
     7081 tttcgccaac agtgcgtcag cggcctggcg aaagcgcagt taagcagtgt ggtcgaagga
     7141 gacaaaaagt cgtggttttg ttcggaattt gtcactgatg catttgccaa agccgggcat
     7201 ccgcttacgc tggcgcaatc gggctggatt agccctgccg atctgatgca tatgcgtatt
     7261 ggtgatgtct ctgcgtttaa gccagaaacg cagttacagt atgttgggca cctgaagcca
     7321 gggatttata tcaaggcggg tcgctttgtt ggtttgaccc ggtgataaag taaatgcctt
     7381 atccgcctta aggtatgagg ggggttgtag gcatgataag acgcgccagc gtcgcatcag
     7441 gcgttgagca ccattgccgg atgcggcgta aacgccttat ccggcctaca aaaaacgtgc
     7501 aaattcaaaa tgttgaaata tatccttctg gcctgttttg cgtttgtttc tgtctcaaga
     7561 cgggttactg ccccaaacta ctgcaatcct ggtttgggta aaatttccct gccgtatcgg
     7621 gtgctaatac tttggttggc gcaccggtat ccggattagc ctcaagcgta aagtgacctt
     7681 ctactgacag taacaccggt ttttcactgt ggccgcgcgc agccaggtag ccacgctcca
     7741 gctctgcgtt attcgctacc atgaaacgtt ttccggtcgc gcaatcagtg aaggtcgccg
     7801 catcagccat ataaaaatac atgccccgca gggtcatcgg cgtcatgggt aaactggatt
     7861 gtgccggttc cagcgtatag ttgaactgcg attcaatcgg attgccttca cgatcgagca
     7921 tctccagcgc atcgcctttc gcccgataat atgacttttc acctttgctg tcggttaata
     7981 ccagcttgtc agcggttcgc gcccatgtac cgtaggaagc gaaggaggaa ggttcttcac
     8041 gagcaccgag ataacgctca ttcatcaccc atgttccgtc tttttcgagg aacagagagg
     8101 tttcgattcc ttcgcaatcg gcacacggca gcacgccgcg ccaactttgc ggcatcggtt
     8161 tcaattcggc agcctgcgcc ggagaaagcg tatcgacttc ggcccgatta ttacaaccca
     8221 tcagagtaaa gaggctgatt acagccatcg ctgtcactat cgcttttttc accatccatt
     8281 ccttcttttt attcccgacc gctgcgcatc gccttcacgc ttgatttttg tgctttcgat
     8341 gccagcctgc gctctttcga tgcacgggtg ggccgcgtgg gtcgtcgggc tttttgttct
     8401 gttgttaaat ctttaatcac tgccaccagc cgggccagcg ccgcttcgcg gttcagttcc
     8461 tgactgcggt attcctgtgc cttaatgaca attacgccat cactgctgat caaatgatgg
     8521 ctggcggcga gcagacgctc tttgtaatac tctggcaggc tggatgcccg aatgtcaaaa
     8581 cgcagatgaa tggccgttga ggtcttatta acatgctgcc cgcccgcgcc ctgcgcacga
     8641 atggcggtga tctcaagctc accatcggga atagcaacat gtcgggaaat cacaatcatg
     8701 aaggttgttg ccaggcggtt aagttcactt ccagattatt cttatcatcc gataaccaga
     8761 tcacgccatc ctgaatcgtt gcctgcagcg tcatggtacg atcggcaaag gcgctcactt
     8821 tcgccagctg ctcgtcgtcc agataccaga cggaaagatt ggcaaactgc gcacatttgc
     8881 tctgattttg ctgccaccag atttgcgccg cccgactatt ataggtaaac agcgccactt
     8941 ctgcggcctg ggtgcaggct ttcttaattc gtcgctcatc cggcagcccc agctcaatcc
     9001 acaaatcaat gcccaggtga tcgttacgca gccacgcttc cggctcatca tcggcacaca
     9061 aaccacgggt aaattgcaga cgttcatcag catatttcag ccacgccagc aggcgcagca
     9121 tcatacgctc ctgggtttct gaagggtggc gcgccagcgt cagagaggca tcgagaaact
     9181 ggttgcggtc gagatcggcc acattaaccg tcgctttata aattgtcgct ttaagcgcca
     9241 taagaaaact cctttcgaat caggaggcca ttgtaacgga atacgtgcag ttgcggcgct
     9301 gatattgcag aaatgagtgt ggtatagtca ccttgcgaaa cagagtttat cttcgtaggc
     9361 ttagacttgc atccacggtt aagtcagagt gctgacagga gggcatgtgg aaaaatattg
     9421 tgagttaata cgcaagcggt acgcggaaat cgccagcgga gacttaggat acgttccgga
     9481 cgcgctgggc tgcgttttga aagtgctgaa tgaaatggct gccgacgacg ccctttctga
     9541 ggctgtcagg gaaaaagcag catacgctgc ggcaaactta ctggtgagcg attatgtcaa
     9601 tgaatgatac gtatcaacca atcaattgtg atgattacga taatcttgag ctcgcctgcc
     9661 agcatcattt aatgctgaca cttgagctga aagatggcga aaaattgcag gccaaagcca
     9721 gcgatttagt ctcccgcaaa aatgtggagt atctggtcgt cgaggccgct ggcgaaaccc
     9781 gtgagctacg tctggataaa attaccagct ttagccaccc ggaaatcggt acggtggtgg
     9841 tgagcgagtc ctgattgact ttcgccggat gcgacgctta atgcgtcata tccggccttc
     9901 actaaactaa gcgtttcgct gccagacaaa acttatgcca ttctcacctt cagccacacc
     9961 ttcttgcgtc acaaatcccc ctgccgccgc aatcagcgtt tcgccataaa acagcagtgg
    10021 cgtggtgtca cgtagccacg gcggcacgcc cagctcttgc cagattttct ttagcttacg
    10081 tccgccgtta cgcccgacaa tatgcagcaa tcctggcgct ttgaaacgca cgctgaccgc
    10141 ttcgtctgca cgcggagggc gaatatcgcc tcccgcatta agctgtacac ttccctgccc
    10201 cgccggtaat tccagcggtt gaagccatgt ctgccacggc acaatgtttt cgctttgccc
    10261 ggtgacggat ttaatccacc acagttgcga ctgatagcgt cggatttcaa acgcgcccaa
    10321 acgtaaacag ggggaggcat cttcccgcgc cagcgccact tcctgccaga tcctcaccaa
    10381 cgcgtcgcgg gaaggcatcg gtgcattctg ccctgccagc cagcggcgga taatcgccgc
    10441 gcggcgggca tcactcatcg ccagcattgg cacaatctgc agcgtcccct gcggcgattg
    10501 acagtgtgct aaatcatctg ccagcagttc atccagcagg ctctcttgtt cagcacaaag
    10561 tgcggcgctg cgggccgttg tttcggcaaa atgcggccaa cgctgctgca ataacggcac
    10621 tacgcgcagg cgcagaaagt tacgatcgta tgagtcgtcc tgattacttt cgtcttcaat
    10681 ccagcgtaaa ccatgcgcca atgcccactg cgccagttcc ccccgcgtgc gggcaagcaa
    10741 cgggcggata agccgtgttc cggcaaactc cgaaacttcc gccatagccg aaagcccggc
    10801 agggccgcta ccgcgtttta gcgccagcag aaaagtttca cattgatcgt cgagatgttg
    10861 cgcggtgacc agcacttcac cgggcaacaa ggtgcgggca aatgcctgat aacgcgcctg
    10921 ccgcgcctgg gcctcaatcc ccagcccctc ttgcgcaagt tgtacgcgtt cgaccaccag
    10981 cggcacctgc cactgttggc agacgttttc gcaatgcgta acccaggcat cggcattggc
    11041 acttaaaccg tgatgcacat gaatagcgcg cagagcgccc cccggatttt ccgtccgcca
    11101 ctgcaccaac tgatgcagca gaacggtgga gtcaagcccg ccgctaaagg ccaccagaat
    11161 ctggcgtgag gtgagaagtt gtctattgag cgtgagtgtc atgatggtgc gattttacag
    11221 tagcaatgcc cggcacgtta ccgggcaaat gcggcgatga caagccttac tgctcataca
    11281 gttccaacgg cagcccgtcc ggatcgttaa agaaggtgaa gcgtttttgc gtgtatggat
    11341 cgacacggat ggcttcacac ttcacgttat ggctttcaag gtgcgccact gccgcatcga
    11401 tgtcatcaac gctaaaagcc agatgacgca ggccgcaagc ttccggtcgg ctggggcgct
    11461 ccggcgggaa cggaaatgag aaaagctcaa tcacatattg cccattaagc gccaaatccc
    11521 ctttccacga gtcgcgcgct tcgcgataga cttcgctttg cagcgtgaaa ccaagaatat
    11581 cgcagtagaa agctttgctc accgcataat ccgtcgcaat aatcgcaata tggtgaacct
    11641 gttttaaacc cagcataacg tctcctttat ttgttaacag cacgttactc gcccggaagc
    11701 cgctctggca agttatcccg ccatttttag gactcgtacg cggtaaacgc cgtcttcgtc
    11761 ctgtttcgca ccgtgaatat ccgtttcaaa accggggtaa tgttgcccta cggaacaaag
    11821 catcagtaga aaatcgagta ctgtgcggct ctctttggtc agcatttctc caggcatcaa
    11881 cagcggtacg cccggtggat aaggcaggat catatttgcc gatactctac cgaccagttg
    11941 ttccagcgca atggtttcta cttcaccttt aatctgtctc tgccatgcct gatgtggcgt
    12001 catgatcatc tccggcaaag tatcgaatgc ccgcaacatc aaaccgggaa gatcgtgttt
    12061 acgaatcagc ttatggatcc cttgcgccag atcctgaata cgcatattgc ggtagaaatc
    12121 gggatcttct gcgtacagat ccggaagcat atttttgatt cgcagattga gatcgtaaga
    12181 gcgtttaaat tccgtcaacc cacgcaataa tcccattgct ttggttttat cgatgccaat
    12241 actaaagaga aacagcaggt tatatgggcc ggttttctct accacgatcc cacgctcatc
    12301 gaggaacttc gccaccagcg ccgccgggat cccctcctcg ctcatattgc cctgctcgtc
    12361 catccccggc gtcagaatag tgactttaac cggatcgagg aacatatgat cggcatccgc
    12421 atcgttaaag ccgtgccatt gttcgccagg cgcaacgggc cagcattcgg cttcatccac
    12481 ctgcggcggt tgccagatat cgaaaaacca accgtcagac tcttcccgca gccgctggac
    12541 ctctttgcga aaatgcagag ctcgttctac tgaacggtta atcagccgtt tgcccggatt
    12601 accacgcagc atcgctgctg ccgtctcaac cgaagcaaca atgggataac tgggcgaggt
    12661 ggtggtatgc atcataaagg cttcgttaaa cgcctcttcg tcatactcac ctttaatgtg
    12721 gatcagcgaa gcctgcgata acgccgccag cattttgtgg gtcgattgcg tttcgaagat
    12781 cacttttccc gcaacacgct cgccgctcat accacttttc ccctggtaga tcggatgaaa
    12841 atgggtgtac ggcacccagg cggaatcgaa gtggatcgac gggacatcca gcgtctgttt
    12901 gatccagtcg gtgttgtaga gcaagccatc ataggttgag ttggtaatca ccgcatgaac
    12961 cggccattgt gcctgcgtgg tggcagcgac tttctcttcg atgctgtcgc gagtaaattc
    13021 acggcgcggg atcccaccca gaatccccaa cgcattacgc gtcggtttca gccagactgg
    13081 cactacatcg ttcatcatca acagatgcgc cagcgattta tgacaattgc ggtcaatcaa
    13141 cagcgtactg ccggatggcg cggcgtacat acccacaatt ttgttcgacg tcgatgttcc
    13201 gttggtaacg atataactct gttccgcgcc gaaagtccgc gcgatgtact cttccgcttc
    13261 caggtgtggc ccggtgtggt cgagcaacga accaagctcg gtgaccgaaa tagagacatc
    13321 agccttaagg gtattcccgc cgaaaaagtc ataaaacaga cagccaaccg ggcttttttg
    13381 atatgcggtg ccgcccatat gacccggcgt acaaaaggtg tacttccgct ctttgacgta
    13441 ggtaaacaag gctttcgtga acggtggggt aatgttatca agatattcgt cggtgtactg
    13501 acgcatacga atggcgatat cttccgcctg ccccagcgca tattcaaaaa aacagagcgc
    13561 catccgcata tcctgcacgc tgacatccat cgtcgagtgg gtgttgatga aggcataaag
    13621 cgggagatat tcattaagct gattgatatc gctacataaa tcgagactgt attcatccca
    13681 gtcaaaaatc acgccgcaaa ttcgagggtt atgttcgata aatttcagca aatcaacgct
    13741 gttttgtggc cagataatct gaaagccttg cgccaccagc gccgactcca gttctttgat
    13801 gggctcatct ttataaaaga cgccatgcgg tcccataatg gcaatgatgt tcatgtgttc
    13861 ctcctggaaa atccttcctt aatcatagcc tgctcaaacc gtggcgataa aaaagggcca
    13921 ccgaagtgac cctttttcag aacttttgcg aattacgcgt aaccgtagct catcaggcgc
    13981 tgataacgac gattttttaa atcttcagtg cttaacacgt cgagatcggc cagatccgcc
    14041 agcagttgcg ctttcaacga tgccgccatc gcttccgggt tacggtgagc gccgcccagc
    14101 ggttccggga tgatggagtc gatcagtttc agctctttca gacgcggagc gatgataccc
    14161 atcgcttcag ccgccagcgg cgctttgtcg gcgctcttcc acagaatgga cgcacaacct
    14221 tccggcgaga taacggaata ggtgctgtat tgcagcatat tcactttatc gcccacgcca
    14281 atcgccagcg caccgccaga accaccttca ccgataaccg tacaaactac cggtacgccg
    14341 aggcgagaca tttcacgcag gttgcgtgca atggcttcag actgaccacg ttcttctgcg
    14401 cccacgcccg gataagcccc cggggtgtcg ataaaggtga tgataggcat cttaaagcgt
    14461 tcagccattt gcatcagacg cagtgctttg cggtaacctt ctggcgctgg cataccaaag
    14521 ttacggcgaa ttttttcttt ggtttcacga cctttttgat gaccaatgat catcaccgga
    14581 cgaccatcga gacgggcgat accaccgacg atagctttat cgtctgcata cgcgcggtcg
    14641 ccagccagtt cgtcaaattc atcaaatgcc aggcgaacgt aatccagggt ataaggacgc
    14701 tgtggatggc gtgccagttg cgcaatctgc catgcaccga gatcggcgaa gattttacgc
    14761 gtcagttcta cgcttttttc acgcagacga tgcacttctt catcgatgtt aatatccagt
    14821 ttctcatcct gacggctaac cgcagtcaga gaatcgattt tcgcttccag ctctgcaatc
    14881 ggctgttcaa aatcaaggaa attcagactc atagtattcc tgtattagtc aaactccagt
    14941 tccacctgct ccgaaccaat gaggccacgg agatcgttta ataaacgatc gctcggagag
    15001 acacgccacg tcgcgccaaa acgcaaccgc gcgcgtgcat ccgccctctg atagtagaga
    15061 tgtactggaa ttgtcccaga gcggtggggt tccagagact gacggagtcg gtttaaaagc
    15121 tggtcatcaa tttgcctgtc cgtcagcgag atagcaagcc cgcgagcata tttttcccgg
    15181 gcttcgtcaa tatccatcac ttcgcgagcg gtcattttaa gcccaccgct gaagtcatca
    15241 aagctgacct gtccgctgac gataagtatg cggtcttttt ccagcaaatg ctggtattta
    15301 tccagggcgt cagtaaacaa catcacttcc agccgcccgg aacggtcatc cagcgtgcag
    15361 ataccgatac gattgccgcg cttggtgacc ataacccgcg cggcaacaac gagccccgca
    15421 gccgtgatga ctttaccacg ttctgtcggg tgcatgtctt tcagccttac gcctccgaca
    15481 taacgctcaa tctcttttaa atactggttg atagggtgtc cggtcaggta caggcctaac
    15541 gtttcacgtt ccccatctaa taccacctgc tccggccacg gttggcagct ggcataggat
    15601 tgttcaattt gttccggctc ttcggccagc acgccgaaca tatcggcctg accgatagct
    15661 tccgctttcg cgtgttgatc tgccgctttt aacgcatcgc ccagcgagtt catcagcgcc
    15721 gcgcgatgtg gcccaagacg gtcaaacgcc ccggacatga tcagtttttc cagcacgcga
    15781 cggttcaact ttttggtgtc ggttcgggcg cagagatcaa acagttcgcg gaagtagccg
    15841 cctttattac gggcttcgat gatcgcctca atcggacctt cacctacccc tttgatcgcg
    15901 ccaataccat acacgatttc gccgtcgtcg ttgacgtgga aatggtaaag accggagttt
    15961 atatctggtg gcaggatttt cagccccatc cgccagcact catccaccag gcccaccacc
    16021 ttctcggtgt tgtccatatc ggcggtcatt accgccgcca taaactctgc cggatagtgc
    16081 gctttcaacc ataatgtttg atatgacacc aaagcatagg ccgcagagtg cgatttgtta
    16141 aatccgtaac cagcgaattt ctccaccagg tcgaagattt tcatcgccag ttcggcgttg
    16201 attccgttct tttctgcacc ttcagcaaat acagaacgtt gcttagccat ctcttccggc
    16261 tttttcttac ccatcgcacg acgcagcata tccgcgccac cgagggtata accagaaagc
    16321 acctgcgcaa tctgcatgac ctgttcctga tacaggataa tgccgtaggt tggctccagt
    16381 accggtttca ggctttcatg ctgccactgc acgtccggat aggagatctc ttcgcgacca
    16441 tgtttacggt cgataaagtt atccaccatc cctgattgca acggaccagg gcggaacagt
    16501 gccaccagtg cgatcatatc ttcgaagcag tcaggttgca gacgcttgat caggtccttc
    16561 atgccgcgcg attcaagctg gaataccgcc gtggtttccg agcgttgcag catgtcgaag
    16621 cttttcttgt catccagcgg gatcgcggcg atatccagcg gcggctcgcc attcttcgcc
    16681 cgccgcttgt tgatcatctc cagcgcccag ttgatgatgg tgagcgtacg caaaccaagg
    16741 aagtcgaact tcaccagtcc ggcgtattca acgtcgcttt tatcaaactg ggtgaccgga
    16801 tgtttgccct cttcatcgca ataaagcggc gcaaaatcgg taattttggt cggcgcgata
    16861 accaccccac cggcgtgctt accggcgtta cgggtgaccc cttccagttt gcgcgccatg
    16921 tcgatcaacg ccttaacttc ttcatccgct tcgtagattt ccggcagctg cggctcggct
    16981 tcaaacgctt tcgccagcgt catccccgga tcgggcggga tcagtttcga gatacgatcg
    17041 acaaagccgt acggatgccc cagcacgcgg cctacgtcgc ggatcaccgc tttcgccgcc
    17101 atcgtaccga aggtgataat ctgcgatacc gcatcacgac cgtacatgtc cgctacgtgt
    17161 tcgataacct gatcgcgttt ctccatacag aagtcaacgt cgaagtcagg catggagaca
    17221 cgttccgggt taaggaaacg ttcgaacagc aggtcaaatt ccagcggatc gaggtcggtg
    17281 attttcagcg cgtaggccac cagtgaaccc gcaccggagc cacggcctgg ccctaccggt
    17341 acgccgttat ctttcgacca ctggataaat tccataacga tgaggaagta gcccgggaag
    17401 cccatctggt tgataacctg aagttcagtc tccagacgtt cgtcatattc cgggcggcgc
    17461 ttaacacgtt cttcctcatc agggaataaa aaggccagac gctcttccag gccctctttt
    17521 gcacgcttga ccagataatc ttcggtgctc atgtccccgg tcgggaactg cggcaggaag
    17581 tattcaccaa gacgcacagt tacgttacag cgtttggcga tctcaacggt gttggcaagg
    17641 gcttctggga tgtcggcaaa cagctcgcac atctcctctt cgctacgcat atattgctgc
    17701 ggcgaatagt tacgcgggcg tttaggatcg tcgagggtaa agccgtcgtg gatcgcgacg
    17761 cggatttcgt gtgcgtcaaa gtcgctgctg tcgataaagc gcacatcgtt agtcgccacg
    17821 acgggcaaac cgcgcgcttc cgccagttcc accgccgcgt gcagatagct ttcttcatcc
    17881 ggcctgccgg tgcggatcag ctcgagaaaa tagcgatccg ggaagtgttc ttcataaaac
    17941 gcgacacact catctaccag cgcgctgtta ccacgcaaaa gactgcgtcc gacgtcgccc
    18001 atgcgcccgc cggaaagaag gatcaaccct tcgtttaatt cgataagcca gtcgcgatcg
    18061 atgatcggcc cggcggcacc gtacccgcgc tgatacgctt ttgagatcag caacgtcaga
    18121 ttctgatagc cggtattgtt cgccgccagt accgtcaggt gggttaactc atcacccagc
    18181 aggtcgcact ggacgttaaa atctgccccg acgataggct taatccctgc gccatgtccc
    18241 gctccgtaga acttcaccag accgcaaagg ttggtgaaat cggtgatcgc cagtgctggc
    18301 atacccaacg ccgccgcctt ttttaccagc ggtgcggttt tggccaggcc atcgatcatc
    18361 gagtagtcgc tgtgcacccg caggtgtacg aaacgtggtt cagacatctt cagattccgg
    18421 tttacttaat ctcgacacaa gaatcaggac gcaagtccca gtgcgcgttt gacaggccca
    18481 aagctgcgcc gatggtgttc ggtcgcgccg tgttcagcca gtttttccag atgaaaagcg
    18541 gttggatacc ctttgtgttg ggcaaaacca tattgcggga aaacaatatc cagcgccgcc
    18601 atttcggcgt cacgcgtcac tttcgccagg atagacgcgg cactgatttc cggtacgcgg
    18661 ctatcgcctt tcaccacagc catcgcaggc atcggtaatt tcgggcagcg gttaccatca
    18721 atcaacacat attccggcgc aatatgcagc ccagcgacgg ctcgctgcat cgccagcatg
    18781 gtcgcatgta aaatattcag ttcgtcgatt tcgtgaggtt ccgcgcggcc cagactccag
    18841 ctcaacgctt tctctttgat ctcttcacag agcgccagac gacgtttttc gctcagcttt
    18901 ttggaatcat tcagcccggc aatcgggcgc gccgggtcga gaatcaccgc agcggtgacg
    18961 accgcgccaa ctaacggccc gcgtccgact tcatccacac ccgcaaccag ctgcgtgtgc
    19021 ggatagacaa attcgatcat tgtgctaact ccagaacggc ttgtgccgcc tgctcatcgg
    19081 cattgcagcg gatctgctga tgcagttcac ggaaggtatc gtgcatcgcg tggctggttt
    19141 tcccattcgc caacagcggt aacagcgccg cagccagttt ttgcggctca cactcttcct
    19201 gcaataactc tttgactaac tctctgcccg ccagcagatt tggcagcgag acataatcag
    19261 ttttcaccag ccgcttcgcc aaccagaagg taaaaggctt catgcgatat cccaccacca
    19321 tcgggcattt cgccagcata cactccaggg ctgccgtccc cgacgccagt agtgccgcat
    19381 cgctggcgac catcgcctca cggcccattc catccagcaa atgaaccgac aggtctggcg
    19441 cgactgcagc tttgatgcgt tcaaactgct cgcggcgttt ggcattcacc agtggcacca
    19501 cgatttcgag atccggatat gtctggcgca aaagctgggc cgttttcagg aaatcggcac
    19561 taagcatttc gacttctgca ccacggctgc ccggcaacaa tgccagacag tgggcatcgt
    19621 aagggatccc cagcacatca cgggcactat ttttatctgg atctaatggc atggcatcag
    19681 ccatggtatg accgataaag cggcacggta cgttgtattt gtcataaaac gctttttcga
    19741 aaggcagaaa tgcgagcacc agatcggtgg ctctgcctat tttgaaaacg cgtttctgtc
    19801 gccacgccca gacggacgga ctgacataat gaatggtttt gataccctgc tttttaaggt
    19861 taccttcgag gataatattg aagtcaggcg catcaatgcc aacaaaaaca tctggcttta
    19921 gttcaccaaa acgctttgtc agatcggcac gaatatgcag taagcgacgc agacgaccga
    19981 gcacttcaac aatgcccatc accgccaact cttccatttc gtaccaggct tcgcagcctt
    20041 cagcctgcat tcgtggcccg gcaacaccaa caaagcgggc gttgggcaca cgttctttca
    20101 gagcgcggat taaaccggcc cccaggatat cgccggaggt ttctccggcg accagggcaa
    20161 tcgttaatgg acgctgttca gtcattaacg aatcagaccg cgcgttgagc gtgcaaagaa
    20221 atcggtaaag gctttcactt ccggatatgt ttccgccagt tcagcaattt ccggtttcac
    20281 ttcatcgagc gttttaccgc tacgataaat cagcttatac gcattgcgga tagcggtaat
    20341 cgcctcacgg ctgaatccgc ggcgcttcag cccttcgata ttgacaccga acggcgtggc
    20401 gtggttaccc tgcgcaatga cataaggagg gacgtcctgc gccacaccgg agcagccgcc
    20461 aaccatcaca tgcgcaccaa tgatgcagaa ctgatggact gcggccatgc cgccgatgat
    20521 cgcgaagtcg tcaaccgata cgtgacccgc cagcgttgcg ttgttggcga gaatacagcg
    20581 gttacctacc gtacaatcgt gcgcaatgtg cgcgttgatc atcagtaagt tgtcgctgcc
    20641 caccttcgtc aatccaccgc cctggactgt gccacgatga atggtgacgc tttcgcgaat
    20701 gcggttacga tcgccgattt ccacgcgggt cggttcgcca gcatatttca gatcctggtt
    20761 aacttcgccg atggaggcga actgataaat ctcattatcg cggccaattt tagtatgacc
    20821 attcacgaca acgtgagatt tcagtacggt accctcacca atttcgacat ggggtccaac
    20881 gatacaaaaa ggaccaatgt gtgcgttcgc gccaatcgac gcgccctctt ccacaatggc
    20941 ggttggatgc acaaaggcgg atttatcaat cacgtatcag gcctcccggc tacgagcaca
    21001 catcatcgtt gcttcgcaaa ctactttacc gtcgaccaga gcaacccctt taaaacgggt
    21061 caggccgcgg cgcgtttttt cgaaagtgac ttccatgatc atttgatcgc caggcacgac
    21121 cgggcgcttg aagcgcgctt cgtcaatacc agcgaagtag tacagctcac ccggttccag
    21181 ttttcctacg cttttaaacg ccagaatacc tgttgcctgt gccattgctt ccagaatcag
    21241 cacacccggg aaaatcggtt ttccagggaa atggccctgg aagaatggct cattgacaga
    21301 gacatttttt actgcgcgca gaaaacgacc ttcttcaaaa tccagcacgc gatccaccag
    21361 taagaacggg aaacggtgcg gcagaagttc taaaatctct tcaatctgca gagtatgagt
    21421 gttagtagtc aagatactct tcctgtcaaa atataagaaa cgacaataat aacacggcct
    21481 gccgcaatcg taagaatgag acaggccgta aagtttggcg aacaaaagat ggaacgttag
    21541 tcttgttgat taaccttgcg ctcaagcgat ttcagacgct tgctcatgtc atcaatgttc
    21601 atcaccagtg cagcggtttt gcgccagact ttgttgggtt gcagcggaat gcctgaggaa
    21661 tagacgcctg gttcagtgat gggacgcatc accataccca tgcccgtaac cgtcactttg
    21721 tcgcatattt ccatatgccc gttgattacg ctggctccgc cgatcatgca gtaacgacca
    21781 attttcaggc tgcccgccat aatgacgcca ccggcaaccg ccgtattgtc gccaatcacg
    21841 acgttatgtg caatctggca ctggttatca atgatcacgc cattgccaat aacagtgtca
    21901 tccagcgcgc cgcgatcgat ggtagtgcag gcaccgatct ccacgcgatc gccaataatt
    21961 acgcgaccaa tctgtgggat cttcacccag ttaccacgat cgttggcata accaaagccg
    22021 tctgcgccta ccactgttcc ggactggatc aggcaattct gaccgatctg gatctcatgg
    22081 taaatggtta cgttcgccca gagacgcgaa cctgcaccga ttttgctgtt tttacctacg
    22141 aagcaaccgg caccgataat cacgttatcg cccagttcaa cgccggattc aatcaccgcg
    22201 ttagcgccaa tcgatacgtt gttacccagc ttcgccgtcg cgtcgatcac cgcactgggt
    22261 gcaatgttct gcgcgggctg cggcgtggta tctaaaattt gcgccatgcg cgcgtaagtc
    22321 aggtagggat tcttcactac cagcgcggca cttttcgcga aaggaagatc gtcctgggtc
    22381 atgacaaccg cggacgcctg gcacaagcct aaatgctcac ggtattttgg gttaaccatg
    22441 aacgtaatgt gacctgtttg tgcagattgc atggacgcaa cgccggtgat gacgatatcg
    22501 ccatcaccgt gtagttctgc atccaactgc tgcgctaaat cagccagtcg aattgaaggc
    22561 attacttatt taacctgttt cagtacgtcg gcagtgatgt cttttacatc gctgctgttg
    22621 taagcaacgg cgtttgcatc aacaaccaga tcgatatcct ggctgttggc aacggatttc
    22681 acagcagtct ggatacgagt aaccagtttg ccgcgttctt cattggaacg acgtgcgcga
    22741 tcctgctcaa aagcctgcgc tttctgagca aaagtctggc gctgagccat cacgtctttt
    22801 tccagcttag tgcgatcgct gcccgctttc atggactgca gctttttcat tttagcctgc
    22861 agatcggttt ccatacgctg cagttcgctg gcacggcctt tgaactcatt ttccagcgtg
    22921 ttagaaacac cggttttctg cgctacctgc tggaacaggc tgcccatgtt gacgattgca
    22981 attttgtcag ccgcctgagc agatgttgcc agtgctaaac cgagacctgc agctaataac
    23041 cactttttca caataaactc cttaccatcc catttgcacc ggagggtgca gttctttgcg
    23101 tggcccggcg atttatattg atcgcctaaa gtcatcgcta cactaccact acattccttt
    23161 gtggagaaca cttaccaggt tttaccgatg ttaaactgga actgttctgc cttgtctcca
    23221 tcgtactttt tgaacggctg ggcgtaggag aacaccaacg gccccaatgg ggacatccat
    23281 tgtaatgcga tacccgcaga catacggata ttgcttggat cactatagtc cggatatcca
    23341 gaatattggc tggaatccca gtttgtatcc caaacggtac ccatatccca gaagaaggaa
    23401 gtacgaaccg agttagcata cttatcgcta ataaacggcg tcggggtgat gaactcgagg
    23461 ctggcaaccg ccatggcgtt accgcctaca gcatcatccg atttacacag gtctttcgcg
    23521 ccgtcctgag tcgcacattc gtaatcatag tccggatcat aattactggc ctgatgcggg
    23581 aagtaaactg ctttcggacc aattgtattg gactggaagc cacgcacggt gctggaacca
    23641 ccggcataga agttctcgta gaacggcatc tctttgccgc ctaaaccatc accataaccc
    23701 cagcgggtac gccccagaac aacccatttg tgatcgtcat cgatcggcac ataagtcgcc
    23761 gtgtctaacg tcactttgta gtattcgtta tccgatccag gaatggtcac tttaccggtc
    23821 aggttgacac gtgaaccatc tgtcgggaag taaccacggt caagcttgtt ataggtccaa
    23881 ccatagttga acgtgaagtc gtccgttttg aagctgttat cctgatcaga ggtgctcgga
    23941 tgttcaccca tagagtacag ataacgccac atcgcaacct gaggctgcat gttggacagg
    24001 gagttatgta cataacccag acctgcacgc agcgagttat attcgttaat cgggaagccc
    24061 aacgtcacgt ctgtaccata actcttgttg gtatagtcgg acaggtcggc gtcatctgcc
    24121 tggaagtcat tatagaagag acgaccaccg aggcttacgc catctacggt gaagtacggg
    24181 ttggttaccg acagttcagc ataggtctgg taatcgtttt tggtcccgtt gataccaaca
    24241 gcataacctg tacctaacca gttatcctgt tgcacgccag cctggaagct cacgccactt
    24301 tcagtaccgt aaccaatccc gaagttgaag ctaccagtgt tacgttcttt caccttgtag
    24361 actacatcta cctggtccgg gctacccgga acacgttggg tatcggtatc gacagtttca
    24421 aagaagccca gacgattcag acgctcctta ccctgatcga ccagatcgct tcccagccat
    24481 gcaccttcca tctgacgcat ttcgcgacgc aggacggcat ctttcgaggt atcgttacct
    24541 tcaaaacgga tcttacgcac gtagaaacgg ttacccgcat caacgttcac gcgtaattta
    24601 acggttttgt cggcatcgtt aatttcaggc atcgactgta cgcgcggata ggcataacca
    24661 tagcgaccga gaagcttttt aatgtcatct tccattttgg tcactttggt gccgttatac
    24721 agctcgcccg gctcgatctt agtcagctgc tcaatttcag cggagtgccc ggcaaggttg
    24781 ccgctcactt caacgccaga aagcttgtac tgatcgcctt cggtgatgtt caccgtgacg
    24841 taaatacctt ttttatctgg cgtcagactg acctgggtag agtctatgtt gaaacgggca
    24901 taaccgcggt ccagatagta gctgcgcagg gtttcaaggt cgcccgccag tttctgtttc
    24961 tggtatttac gatcgcctac cacgttccac cacggcactt cgtcacgcag ttggaaatga
    25021 gagatcagtt cgtcggtggt gaaagcatgg ttaccaacaa tgttaatttg ctggatttca
    25081 gctgacacac cttcctggaa caccagtttt aggtcaacac ggttgcgcgg cagcggggtc
    25141 acgacagctt taacgctggc gctatattta ccgacgctgt agtagaagtc ttccagacct
    25201 ttctcgatat cggcaatggt ggtgcgatcg agggattcgc ccacacgcac accagaagcc
    25261 tcgaggtttt gcttcagcat gtcatctttc accgatttgt taccggagaa agtaatgctg
    25321 gcaatggtcg gacgttcttt tacctgaacc agaagggtat caccatcacg aaggacgcga
    25381 acatcctcaa agttgccggt agcaaacaga gcgcgaatgg tattactgat atcttcatca
    25441 ttaaccgtgt cgcctgtgcg caccggcata ctgaggaggg ccgcaccaac ggcgacacgc
    25501 tgaaggcctt cgaaatgaat atctttcact acgaaccctt cagcaccgta tacggtggcg
    25561 ctgctaaaca gcagcgacgc tatgagcaac tttttcatcg ccatcgttat tatgcgttct
    25621 tcctaactaa ctctcataac cgagagaaat cattgaaaag tgcaagcccc attaacagca
    25681 ccagcagaat cgagccaatg cgataacaaa agtcttgaac ccgctcggat accggtccgc
    25741 ctttgatctt ttcgatcgca aggaacagca gatgcccccc gtcaagtacg ggcaacggaa
    25801 acaggttaat tatccctaag ttcacgctaa taagcgcaag aaacggcagg taataaacta
    25861 ccccgagttc cgctgtcatc ccagccccct tggcgataga gatcggccca ctgaggttgt
    25921 tcagtttcac atcaccggtg atcaattttc ccagcatact gaccgtcagc ttcatcagct
    25981 gccacgtttt gtccgtggct tcgacgatgg cgttgaacgg cccatactgg cgtacaactt
    26041 tatactcatc tggcaaagga atgactttcg gctcaatacc gacaaaacca atcgctttac
    26101 cattacccgg tttactctcc gggattaatg tcaaagacaa gggactcccc tgcctttcga
    26161 tttctaacgc taaggattta cccgggttat cccggacaag catcacaaag gtcacccact
    26221 gcgttaaggg ctgaccatcg actttaacga tcctgtcgcc tgcttgcaaa cctgccttgc
    26281 ttgccgccga gtttggctgc acattttcca gtacaggttc aatttgcggc ccacgaggac
    26341 gaatccccag cgaagatacc ggatcttctt tatcaggctc aaacgcccag tgacgtaaat
    26401 cgagctttac atcccgccgt tggtcgctgc caaatggcgc tactgtaatg gtggtgcttt
    26461 catcgccaat tttatcgacc aactgcaaac gcacggcatc ccaatcaggc gtttcgatac
    26521 catctacggc ttttagttcc gtacctggtg caatttgtgc ttccgcagct atcgaattgg
    26581 ctgctatttc gccaaccacc ggacgtacgc caggcacacc aataataaaa accagccagt
    26641 aggcaaagat agcaaaaatg aagtttgcaa ccggacctgc ggcaataatc gccgctcgtt
    26701 ggccgacaga tttattattg aaggcatggt ggcggagttc cggaacgacc ggttctgcgc
    26761 gctcatccag cattttgaca taaccgccca acgggatcag ggcgataaca tattcggtgc
    26821 cgagcttatc agttcgccgc cagagcgcct taccaaaccc tattgagaaa cgctcaacgc
    26881 gaacaccaca acgccgggca acccagaaat gaccaaattc atgcacggtg ataagtacac
    26941 ccagtgcaac gatgaacgaa gccaaatccc agagaaaact cagcatatta ccttccgtta
    27001 aagcgtcctg aataccagta acaacaagca agcaaagacc ggtaccgcag ccgtcaggct
    27061 atcaatacga tctaaaatac caccgtgtcc tggaattaaa tgaccgctgt ccttaattcc
    27121 tgcttcacgc ttaaacatac tctcggtcag atcgccgagc actgaggcta acgctgcgac
    27181 aatagagcaa atgagtaagg tgacgggagc gacgtcgaga ttcgcccaca tgccataacc
    27241 ccatgagatt accgctgcag tagcgagtcc accgataaag ccttgccagg ttttacccgg
    27301 agaaaccttc ggtgccagct tatgtttacc aaacaatttg ccaaacatat atgcgccgga
    27361 gtcagcgccc cataccagga tcatgacata gagcagccat attgcgccac tgtaatgatt
    27421 ctcgtcatag tgccaggccc gtaacgccag catgccccag aagaagggaa caatggttag
    27481 cacgccaaaa ataaggcgca atgttttaga gttacgccag attgctgcgg aacctgggta
    27541 aaacagcacc agcaatagcg cgacaatcca ccaacccagc gaagcccaaa gtgagatttc
    27601 aaccagcggt tgatgaatat ttcggtgata ttccggcaac agaaaaagca tcagcgccaa
    27661 caataacccg cataacaccg ccaaccatac tcgctgcgaa cgagtggtaa aaccgctaag
    27721 ctgtccccat tcccacgctg ccagcatgca gaccaccagc gttacaatgg cgaaccccac
    27781 cggcggcaac agaaacaacg ccgcgatgac gacgggtatt aacacaaaag cagatatcag
    27841 gcgatacttc agcaaaagcg acccccatca ggctgtttca tcaccgggct cggtgccgcc
    27901 gaaacgacgc tctcgattag caaaggcatt taacgcccct tcaaagtctt gttcatcgaa
    27961 atcgggccag agaacatctg taaagtaaag ttcggcatag gcaatttgcc aaagcaaaaa
    28021 gttactaatg cgatgctccc ccccagtcct aattactaaa tctacagggg ccagttcatg
    28081 catacagaca tgctggttta gcatctcttc atctatctga tctggttgca ggtttccttg
    28141 ctgcaccttt tcagccagtt gcctgactcc ctggactata tcccaacgtc caccgtagtt
    28201 cgccgcaata ttcagcgtca gaccggtatt cccggctgtt agcgcttcag atttacgaat
    28261 acgttcttgc aaacgcgagt taaagcgact ggtatcgcca ataatacgca gacgcacgtt
    28321 atgtcggtgc agacttttta cttcgctatc gagcgcccac acaaacagtt ccattaacgc
    28381 actgacttcc tgcgctggtc ggttccagtt ttcactacta aaggcataca gcgttaacgc
    28441 ctcaataccg ttgttggccg caaaagagac agcccggcgg acggattttg ctccggcttt
    28501 atgcccaaag gcacgaatct tcccttgctt ttttgcccag cgaccattgc cgtccataat
    28561 gatcgcaaca tgacggcagc catgcgctgg caatttttcg ctaagtggtt gagtagcaga
    28621 caacatcacg cgttttttat tccctgacag ggtttacggt actcaggaat actgaagcct
    28681 atacaaaaaa aagccgtgta aaaccacggc atacctgacc ttataaagcc aactacttac
    28741 gatcagatgg cgcagactat atcactgaag ccctacgcta acaaatagcg cgactctctg
    28801 tagccggatt atcctcagct tgcgagacgc atcacctctt ttctggcgac ttcacgcgcg
    28861 ctcgcatcaa cagataacac atcgtccaca cattgtggtt cgcgcatatc cattttttcc
    28921 agtacggata aattcaacgc agcgatatcc gtaaagcgga tttgttgcgc aagaaaagca
    28981 gcaacggtga tttcgtttgc ggcattcaat gctgtcgtcg ctgcctggcc ttgttcgaac
    29041 gcctccatcg ccagtttcag gcatggataa cgatcataat ccggtgcggc aaatgtcaac
    29101 gcacttagtt tgcaaaaatc gagcggcttc acgccagagt tcacgcgatt cggccatgcc
    29161 atggtgtggg caattggcgt acgcatatcc ggttccccca gctgcgccag aacactgccg
    29221 tcctgatagc gcaccattga gtgaatcact gactgcgggt gaatcagcac ttccatctgg
    29281 ctggcgctgg cgttaaacag ccaacgcgct tcaatgtatt ccagaccttt gttcatcatg
    29341 gtagccgaat cgacagaaat tttacgtccc atcgaccagt tcggatgacg gcaggcttga
    29401 tcgggcgtca ttgttgccaa atcgcgcaat ggcgtctcac ggaaagggcc accagacccg
    29461 gtaagtaaaa tggacaccac gccattttgc tcaaggtcag cgtatcccag attatgctgg
    29521 ataggttgcg gtaaactctg aaaaatggcg ttatgttcgc tatcgaccgg taacaattgc
    29581 gctttgctct gctttacggc gtccataaac agacgtccgc aggtaaccag tgattcttta
    29641 ttggccagca aaatggtttt acccgcgcgg atcgcagcaa gcgtaggtaa cagcccagca
    29701 gcgccaacaa tggctgccat cacctgatca acatcctcaa gcgctgccat atcgcaagcg
    29761 gcttgttgcc cacttaagac ttcggtgcgg ctaccctgtt gctgtagcat cgttttaaga
    29821 agtttcgcac tcgcttcatc gtccattacg gcatagcggg gagagaattc caggcactgt
    29881 tctaccatgc gagtgacatt tttgcctgcc accagcgcaa ctacgcggaa gtgttcggga
    29941 ttatggcgca ccacgtccag cgtgctgcaa ccaatcgagc cggtcgagcc cagaatggtg
    30001 agttgcttca tgaaacatcc agagttgaga cagaataaaa agcaaaacgc cgccagccga
    30061 tccgcaagga tctactgagc ggcgtttttg tcgttcaaga aatcagaact gcatcagttc
    30121 tgcttctttg tctgccagcg ccgcttcaat tttcttgatt gcagcatcag tcagtttctg
    30181 tacatcgtcc tgagaacggc gatcgtcgtc ttcgctgatc tctttatctt tcaacagtgc
    30241 tttcactttg tcgttcgcgt cacgacgcac gttacgtact gcaacacgcg cttgttctgc
    30301 ttcaccacga acgattttgg tcagatcttt acgacgttct tccgtcagcg gcggcagcgg
    30361 aacacggatg tcgctacccg cagagttcgg gttcaggcca agatcggacg ccataatcgc
    30421 tttttcaacg gccggagaca ttgaacgatc aaacacgttg attttcagag tacgggaatc
    30481 ttctaccgtt acgcttgcca gctgacgcag cggcgtcggc gtgccgtaat attccacgac
    30541 aatgccatcc agcaggctgg gagaagcacg acccgtgcgt attttgctga tttgggtttt
    30601 gaacgcttct acgcatttgt ccatgcgtac ttcagcatct tttctgatat cgctaatcac
    30661 gttacgaatc cttgaaaact tgtctcagtc agaccagaca gtcacacaca gtggaagtgt
    30721 gctaagtata gccctgatta aacatattac gggtaagatc ccccgcgata cttacgcgga
    30781 atcttaccct tatttatcca tcacgggaat tattccgtga ttaaagtccc ttctttttca
    30841 cccattacca cacggcgcag cgcacccggt ttgttcatgt tgaaaacacg aatcggtaat
    30901 ttatggtcac gagccagcgt gaaggccgcc aggtccatga ctttcagctc tttttccagc
    30961 acttcgctgt aagtcagttg ctcgtacatg gttgcggttg gatctttcgc cggatcagcg
    31021 gtaaacacgc cgtcaacttt ggttgctttc agcaccacat cggcttcaat ttcgatacca
    31081 cgcaggcaag ctgctgagtc ggtggtaaag aacgggttac ctgtaccggc ggagaggatc
    31141 accacacggt tgttgcgcaa caggctgata gcttctgccc agctgtagct gtcgcacacg
    31201 ccattcaatg gaatagcgga catcagacga gcgttcacat aggcgcggtg cagtgcatca
    31261 cgcattgcca ggccgttcat tacggtcgcc agcatcccca tgtggtcgcc cacaacgcgg
    31321 ttcatacccg ctttcgccag accagcgcca cggaacaggt tacccccacc aatcaccaca
    31381 ccaacctgaa tacccagttc aaccagttct ttgatttcct gagccatacg atccagtatg
    31441 cttgcatcaa taccgaagcc ttcagtgccc tgcagagctt cgccactcaa cttaagcaga
    31501 atgcgtttat agacgggttt tgcattggta gccatgtttc tttcctgaga ctgtcaacga
    31561 ttgaaatgaa ttaatactag cgacattgta cgtcgctttt cagcgcagcc ccacaagggt
    31621 tagctgaatt tacaagatgg gcacaaaaag aagccgccct caggcggctc ctttttgata
    31681 attaagactg cttggacatc gcagcaactt ctgctgcaaa gtcagtctca actttctcga
    31741 tgccttcacc cacttcgaag cggatgaagc cagtcacttc agcgttatgc tctttcagca
    31801 gctgaccaac agttttgctt ggttccataa cgaacggctg accggtcaga gaaacttcgc
    31861 cggtgaattt cttcatgcgg ccttcaacca ttttctctgc gatttctttc ggcttaccag
    31921 actgcatcgc gatatccagc tgtacctggt attctttttc taccacttca gcggatacgt
    31981 cttccggttt gatgaattct ggcttgcttg cagcaacgtg catagcgatg tgtttaacca
    32041 gctcttcgtc agcgccttta gcagcaacca gaacgccgat acgcgcacca tgctgataag
    32101 aacccagaac gtcgccttcc agcgcagcaa cgcggcgaat gttgatgttt tcaccaattt
    32161 tcgctaccag cgcaacacgt tcttcttcga actgtgcttt cagaacttca acgtcagtga
    32221 ttttgccagc aacagctgcg tccagaactt tgtctgcgaa cgcctggaaa ccagcgtctt
    32281 ttgcaacgaa gtcagtctgg cagttaactt ccagaatgat gccgtagttg ccgtcgattt
    32341 tggttttgat cacgccgtca gcagcaacgt tgcctgcttt tttcgctgct ttaatagcac
    32401 cggacttacg catgttttcg attgccagct cgatgtcgcc gttagcttca gtcagtgctt
    32461 ttttgcaatc catcatgcct gcgccagtac gctcacgcag ctcttttacc agggatgcgg
    32521 taatttcagc cattctaaaa tcctcggaga tgtgatctgc ccggcattaa accgcacaga
    32581 tataaaagtg aaaaaggggg ccatatgcag gccccctaac caaacgtgta ctacctggtc
    32641 tataagggct ctcccccttc atatttcgcg ccgcagatgc gtcatcttcg ctcgctcatc
    32701 ccggtcactt actgatgtaa gctcccggga attctcgagc ttgccgcctt tctgcaactc
    32761 gaactatttt gggggagtta tcaagcctta ttactcagct tctacgaagc tttcttccgc
    32821 ctgggaagcc agatcctgag aacggccttc acgtacggtt gcagcaacag cgcccaggta
    32881 cagggtcaca gcacggattg cgtcgtcgtt acccgggata acgaagtcaa caccgtccgg
    32941 atcagagttg gtatcaacga tagcaaatac cggaataccc aggttgtttg cttctttgat
    33001 agcaatgtgt tcgtggtcag catcgattac aaacagagcg tccggcagac cgcccatgtc
    33061 tttgataccg cccaggctgt tttccagttt ctccagctca cgagtgcgca tcagcgcttc
    33121 tttcttggtc agcttgtcga aagtaccgtc ctgagactga gtttccaggt ctttcagacg
    33181 tttgatggac tgacgaacgg ttttccagtt agtcagcata ccgcccagcc agcgatggtt
    33241 cacgaagaac tggtcgcagc tcagagcagc gtctttcacc gcttcgcttg cagcgcgttt
    33301 agtaccaacg aaaaggattt tacctttgcg agaagcaatc ttgttcagtt cagccagagc
    33361 ttcgttgaac atcggtacag ttttctcaag gttgatgatg tgaactttgt tacgcgcacc
    33421 gaagatgaac ggcttcattt tcgggttcca gtaacgggtc tggtgaccga agtgaacacc
    33481 agccttgagc atgtcgcgca tggaaacagt tgccatgatt aaaacctcta tataaaagtt
    33541 ggggttatgc ctccacgtat cccatattac cgaccccaaa gggcaccccg gaatatgtgc
    33601 cgatacgtgt gtgttgttac acaaagtgag attgtcgctt ccgtccagtc tgtgtatacg
    33661 aaatggatcg gaagtccggc gcgctttata ccacaaatac gccgtggaca ccaataattg
    33721 ttggcgctgt gtacagcatc agacgtcgaa ttttctatta tagaaaacct tcagtggcac
    33781 gtttggcgaa attcagaatg attctcaatt tgcccgggtg tgataccatt gacggcactt
    33841 acatatatat tgtcggtatc accgacgctg atggacagaa ttaatggcta tctcaatcaa
    33901 gaccccagaa gatatcgaaa aaatgcgcgt cgctggccga ctggctgccg aagtgctgga
    33961 gatgatcgaa ccatatgtta aaccgggcgt cagcaccggc gagctggatc gcatctgtaa
    34021 tgattacatt gttaatgaac aacacgcggt ttcagcctgc ctcggctatc acggttatcc
    34081 gaaatccgtt tgcatttcta ttaatgaagt ggtgtgccac ggtattccgg acgatgctaa
    34141 gctgctgaaa gatggcgata tcgttaacat tgatgtcacc gtaatcaaag atggtttcca
    34201 cggcgatacc tcgaaaatgt ttatcgtcgg taagccgacc atcatgggcg aacgtctgtg
    34261 ccgtatcacg caagaaagcc tgtacctggc gctacgcatg gtaaaaccag gcattaatct
    34321 gcgcgaaatc ggtgcggcga ttcagaaatt tgtcgaagca gaaggcttct ccgtcgttcg
    34381 tgaatattgc ggacacggta ttggtcgcgg cttccatgaa gaaccgcagg tgctgcacta
    34441 tgactcccgt gaaaccaacg tcgtactgaa acctgggatg acgttcacca tcgagccaat
    34501 ggtcaacgcg ggtaaaaaag agatccgcac catgaaagat ggctggacgg taaaaaccaa
    34561 agatcgcagc ttgtctgcac aatatgagca tactattgtg gtgactgata acggctgcga
    34621 aattctgacg ctacgcaagg atgacaccat cccggcgata atctcgcacg acgaataaaa
    34681 tgaagccggc gaatgccggc ttttttaatg cgataattta atcttatggg tggcgcacaa
    34741 tgaataccct tccagaacag tacgcaaaca ccgctctccc caccctgccc ggtcaaccgc
    34801 aaaatccatg cgtctggccc cgtgatgaat taaccgtcgg tgggataaaa gcccatatcg
    34861 atactttcca gcgttggctg ggtgatgcct ttgacaatgg gatctctgca gaacagttga
    34921 ttgaggcgcg caccgagttt atcgaccagc tcctgcaacg attatggatt gaagcgggat
    34981 tcagccagat tgccgacctg gcattggtcg ccgtcggtgg ctacggtcgt ggcgagctgc
    35041 atccactttc tgacatcgat ttactgattt taagccgtaa aaagctcccg gacgatcagg
    35101 cgcaaaaagt gggcgagctg ttaacgctgc tctgggatgt aaagctggaa gtcggtcata
    35161 gtgtgcgcac gcttgaagag tgcatgctgg aagggttatc ggatttaacc gtcgccacca
    35221 atttgatcga atcccgctta ttaattggcg atgttgcgct atttcttgaa ctgcaaaaac
    35281 atattttcag cgaaggattc tggccttccg acaagttcta cgcggcgaaa gttgaagaac
    35341 agaaccagcg ccatcagcgt taccacggca ccagctacaa tcttgaacca gacatcaaaa
    35401 gtagccctgg cggcttgcgc gatatccaca ctctgcaatg ggtggcccgc cgtcattttg
    35461 gcgcaacatc gctggatgaa atggttgggt ttggcttctt aacctcagcg gagcgggcgg
    35521 aattaaacga atgtctgcat atattgtggc gtattcgctt tgccctgcat ctggtcgtca
    35581 gccgttacga taatcgcctg ttattcgatc gccagcttag cgtcgcccag cgtctgaatt
    35641 acagtggcga aggtaacgaa ccggtcgagc ggatgatgaa ggattacttc cgcgtgacac
    35701 gccgcgtcag tgaactcaac cagatgctgc tgcaactgtt cgatgaagcc atcctcgccc
    35761 ttcccgccga cgaaaaacca cgtccaatcg acgatgagtt tcagctacgc ggtacgctaa
    35821 tcgacctgcg tgatgaaaca ctatttatgc gccagccgga agccatcttg cgtatgttct
    35881 acaccatggt gcgcaacagt gcgatcaccg gcatttactc caccacactg cgccagttac
    35941 gccatgcccg tcgccatctg caacaaccgc tgtgtaatat tccgcaagca cgaaaactgt
    36001 ttttgagcat tctacgtcac cccggagcgg tgcggcgcgg gctattgcca atgcatcgcc
    36061 atagcgtact cggcgcgtat atgccgcaat ggtcacatat cgtcgggcag atgcagtttg
    36121 atctgttcca cgcctacacg gtggatgaac atactatccg cgtgatgctg aaactggaga
    36181 gttttgccag tgaagaaacg cgccagcgcc atccgttgtg tgtgaacgtc tggccgcgcc
    36241 taccgtcaac tgagctgatt ttcatcgccg cgctgtttca cgatatcgcc aaaggacgcg
    36301 gcggcgacca ctctattctc ggtgctcagg atgtagtgca ttttgccgaa ctccacgggc
    36361 tgaactcacg cgaaacacag ctggtcgcct ggctggttcg ccagcacttg ctgatgtcgg
    36421 tgacagctca gcgtcgcgat attcaggacc cggaagtcat caagcagttt gccgaagaag
    36481 ttcaaacgga aaatcgtctg cgctatctgg tatgcctgac agtggctgac atttgcgcca
    36541 ccaacgaaac gctgtggaat agctggaagc aaagtctgtt gcgcgaactc tatttcgcca
    36601 ccgaaaagca gctacgacgc ggaatgcaaa acacgccgga tatgcgcgaa cgggttcgcc
    36661 atcaccaact ccaggcgctg gcactactgc gcatggataa catcgacgaa gaggtgctgc
    36721 accaaatttg gtcacgctgt cgcgcgaact attttgtccg tcatagccca aatcaactgg
    36781 cctggcatgc gcgccattta ttacagcatg atttaagcaa accgctggta ttgcttagcc
    36841 cgcaggctac gcgtggaggc accgagattt ttatctggag cccggaccgc ccttacttgt
    36901 ttgctgctgt ttgtgccgag ttagatagac gcaacttaag cgttcacgac gcacaaattt
    36961 tcaccactcg cgacggtatg gcgatggata cctttatcgt gctggaaccc gatggtagcc
    37021 cgctatccgc agatcgtcat gaggttattc ggtttggtct ggagcaagta ctgacacaaa
    37081 gtagctggca gccaccacag ccgcgtcgtc aaccggctaa actacgtcac tttactgttg
    37141 aaaccgaagt gacgtttttg ccaacccata ccgaccgcaa atcgttcctt gagcttatcg
    37201 cgctcgacca gccaggactg ctggcgagag tcgggaaaat ttttgccgat ctgggaattt
    37261 cgcttcatgg tgcccgaatt acaaccattg gcgagcgagt agaagattta ttcataattg
    37321 ccaccgctga ccggcgtgca cttaataacg agttgcagca ggaagtgcat cagcggttga
    37381 cagaggccct caatccaaac gataaagggt gatgtgttta ctgatatgaa aagagtttaa
    37441 caatgcagca gttacagaac attattgaaa ccgcttttga acgccgtgcc gagatcacgc
    37501 cagccaatgc agacaccgtt acccgcgaag cggtaaatca ggtgatcgcc ctgctggatt
    37561 ccggcgcact gcgtgtagcg gaaaaaattg acggtcagtg ggtgacgcat cagtggttga
    37621 aaaaagcggt gctgctctct ttccgtatta atgataatca ggtgatcgaa ggggcagaaa
    37681 gccgctactt cgacaaagtg ccgatgaaat tcgccgacta cgacgaagca cgtttccaga
    37741 aagaaggctt ccgcgttgta ccacctgcgg cggtacgtca gggtgcgttt attgcccgta
    37801 acaccgtgct gatgccgtct tacgtcaaca tcggcgcata tgttgatgaa ggcaccatgg
    37861 tcgatacctg ggcgaccgtc ggttcttgtg cgcagattgg taaaaacgtc cacctttccg
    37921 gtggcgtggg catcggcggt gtgctggaac cgctgcaggc taacccaacc atcattgaag
    37981 ataattgctt catcggcgcg cgctctgaag tagttgaagg ggtgattgtc gaagaaggtt
    38041 ccgtcatttc catgggcgta tacattggtc agagcacccg tatttacgac cgtgaaaccg
    38101 gcgacatcca ctacggtcgc gttccggcgg ggtctgtggt tgtttcaggt aatctgccgt
    38161 cgaaagatgg caaatacagc ctctactgtg cggttatcgt taagaaagtt gacgcgaaaa
    38221 ctcgcggcaa agtcggcatt aacgaactgc tgcgtaccat cgactaaaag tatgcacacg
    38281 ggcagcacga cgctgcccga ttgctttctg gttcagaata attcgtccta ctcattcttc
    38341 ctcccccctt ttttaaatac tttttcatct tgtttcggac tgtcatgtaa ttcgcaaaac
    38401 gaagataaat gtgctaatcc atcccatgga atatcgccac aataaagcag gtaatttttt
    38461 gatttttacg ccatgataga agtctggaag aaaaaggctt tctgcgatca cagtcgagcc
    38521 gggcgggcca ggccaaatgc taaaataagc acattttact gactcatttt gaggtaacgc
    38581 tatgtacgac aatctgaaaa gtctgggtat taccaatcct gaagaaattg atcgttacag
    38641 cctccggcag gaagccaaca acgatattct gaaaatctat ttccagaaag acaaaggcga
    38701 gtttttcgcc aagagcgtta agtttaaata tccgcgtcag cgtaaaacgg tcgtcgctga
    38761 tggtgtgggt cagggttata aagaagtcca ggaaatcagc ccgaatctac ggtatatcat
    38821 tgatgagctt gatcaaatct gccagcgtga ccgcagcgaa gttgatctta agcgtaagat
    38881 cctcgacgac ttacgtcacc tggagtcagt cgtaaccaat aagatcagcg agattgaagc
    38941 cgatctggaa aaactaacgc gtaaataatc ccgaattgac ggttaactcg ggcattcccc
    39001 tctccccttc gggctgaggg gaatgagcca gcgccgcctc aaccttaacg cataacctac
    39061 cgctcttcat ccagttgtaa cgccacatac agcagcaacc tgtcatcaaa attgcccaaa
    39121 tcaagcccgg tcagttccga tatacgatta agccgatact ccagggtatt acgatgaata
    39181 aacaacgcct ttgacgttgc cagcggttgc acattgtgac gaaaccacgc cgccagcgtt
    39241 cgtcgcagca agccgttatt gtccatcgct ttcagccgcg ccagcggtcg cgccagttcg
    39301 ttggcctgcc agtcgccacg caaactgtcg agtaacacag gtaacatcag atcctgataa
    39361 aaatagcagc gactttctgg catccgctgt ttacccacca ccatcgtcgt tttcgccgta
    39421 cgataggatc gggcaatact gccaggaccg gtaaaatagt tgcccagtga aacgcgaaaa
    39481 cgcagctggc cgtactcttt catgcgggta atcagttgtt caactcgctt acgatgatct
    39541 tctacatccc agcgcccaaa agagttcaac gccggtttca acaccaccat ttcggttagc
    39601 gagacaatcg ccaccagatt attacgctcg ggcgtagtca gcgcgttttg cagttgttgt
    39661 aactccgcca ttgcgctgtc cacaccaagc tgaccgctgt cgacctcaac aatagccacc
    39721 actcgcggtt gattgagatc gatccccagc cgttgcgccc attcagtaag tgcgggagta
    39781 ttctcctctg cctgaatcag gttcatcacc agttcctccc gcaaacggct atcctgcgcc
    39841 aacaagtgca tcaaccgcga ctgttccagc atcatttcag ccgtcatgca gaccagttcg
    39901 ccatatttac gcagattctc tggttcacct gtcaggccaa ttacgccgac aatttcacct
    39961 tccagccgta acggtagatt aatcccttgc cgcacaccgt gcagatgacg tgccaccgcg
    40021 tcatcgatat cgacgactcg tccctgtgaa agtaccagca atgcaccttc gtgcaattca
    40081 ccaatacgct cacgatcgcc gctgccgata attcgcccac gggcatccat tacgttgata
    40141 ttggtatcga tgatgcgcat ggtacgtgcc acgatatcct gcgccatttt ggtatcaaga
    40201 tgccagccag ccataagtcc tccgttatgc acggcttagc ataaggaagt acgtaacgta
    40261 cggcattgtg caaatgccta aaggatgatg aagatgtatg gagttgtggt gaagttcaca
    40321 gattgtaagg agaccccctt cccgttttca ggaaggggtt gagggagatt actgcattaa
    40381 caggtagatg gtgctgtcgc cgcgctgaat gttcagtgcc agcacagacg gtttgctgtc
    40441 gagaacttta cgcagttcag cgatgttttt cacagcctgc tggttcgcgc caataatcac
    40501 atcacctttc ttcaggccga tctgcgcagc cggagtgccc gttttcacgt tgttcactac
    40561 cacgccctga tctttgcctt tgttgctcat ctcagcgcct tcaatgccgt tgaagatgga
    40621 gctggaatca acctgattct ggctgctctg ctgcagttcc agattcacgt tgacctgctt
    40681 accgtcgcgc agcaagccca gggtcagttt gctgcccacc ggcatagtac ccacttgagc
    40741 acgcagtgcg gcaaagctgc tgatcggctt accgttcagt gaggtgatca catcacccgc
    40801 tttaatgccc gcttttgcag cggaggaatt aggcagaacc tggcttacga aagcaccgcg
    40861 ctgggcgtca actttcatcg ctttcgccag ttcggagttc agctcagtcc ccataatacc
    40921 cagctcaccg cgtttcacct ggccgtattc caccatctgc gaggtcaggt ttttcaccat
    40981 gttactcggg atagcaaaac cgataccgat gttaccgccg tccggtgcta ggatcgcggt
    41041 gttgataccg atcagttcgc cgttcaggtt aaccagcgca ccaccggagt taccacggtt
    41101 gatcgctgca tcggtctgga tgaagttttc gtagttttcg gcattcaggc cgctacgccc
    41161 cagcgcagag acaatcccgg aagttaccgt ctcgcccaga ccaaacgggt taccaatcgc
    41221 tacggtgtaa tcacccacgc gcagtgcatc agaatccgcc atcttaattg cggtcaggtt
    41281 tttcggattc tggatttgga tcagcgcgat atcagagcgc ggatctttgc caaccatctt
    41341 cgcgtcgaac ttacggccat cgctcagttg aactttaatg accgtcgcgt tatcaacaac
    41401 gtggttgttg gtgacgacat agcctttatc ggcatcaatg atgacgccgg aacccagcgc
    41461 catgaatttc tgttgctggc cgccaccatt accgccctgg ccaccctggc agaacggaga
    41521 gctctggaac ggagaacctt cctggcagaa cggagaatca tcaccgaaga actgctggaa
    41581 attacgcggc atacgcggcg tattaacggt tgtgctacct tctacgttaa tgctgaccac
    41641 tgaaggcatc accttttcga gcatcggtgc aaggcttggc atctgctggg ctgtcgttgc
    41701 tgaagaagtc tcagccgccg ttgcagagag cggagataac gccaaaccta aactcagagc
    41761 cagtgcactc agtgctaatg tggttttttt catgtatttc agtctcgatt aacagataac
    41821 gcaaaattgc tgtgttcttc agattcgttt tatagcgcga agttccgaac taaagtttct
    41881 gcaaaaggta aaaatttatt gttcgtcttt acaaaagcct ggttattgtt ctacggccat
    41941 cagacgtcgg tattcatccc aggcatagag gtcggtcata ccgctgatat aatcctgcag
    42001 caggcggcaa cggtaataat attcccatag cggaaactca ggagaatctg acggtaattt
    42061 actgacagcc tcgacatagg ccagccgatg gcgcgtcgag agtttgtgga ataagcgaga
    42121 ttcaataggg gaacgtttca cccgttcttt ttctaccagt tcagtaaagt ctgataatga
    42181 caggcttaat aaagggcgat aaatctctaa taatccgcta atgacccgat agccctgcaa
    42241 ttcaagctgc tcgacatctg gatggctaaa cacatgtttt acagcgacat ttttatatag
    42301 cttaagaaga tcgctgcatt cgctggcatc ttccaataat gcatgattaa acgttccggc
    42361 gaaaatcgca ggcagattat caataaatcg ttgtgccgcg tagggtacca gtttatttag
    42421 ggtgtttacc cgtaaataca taaaaaactg atcttccgta ctgcggctta aactatttga
    42481 gcgtgatttt tcccaggcat tttcaaccac cagcgaaaag agcgaacctt tctcatgctg
    42541 gccccacgcc tcgtgcaaat gatgataaag ctgctcaacg gtaaatattc ttttctctac
    42601 cgcatcttca agatctgcca cacaatagga gatgtcgtcg gcagcttcca taatccacgt
    42661 taatggaaaa cgactgtaaa gcgccaaatt aagttcttta cgcaaccggg caatataggc
    42721 ttcttcagaa agataataac ccggcttttt cattaaatag tgatgtgtct caggcgtttc
    42781 gccacgccac cacgctggac gggtatattt taaaataccg ccaacctgcg cccaggtgag
    42841 attcatccgc atcaatgtat gcaccagacg aatgccttgt gcattcccct caaaatgaca
    42901 taagtcctga cgaatcttgc gccgcagctc gttaagcggt tcttccccgt cccgtaaacg
    42961 tagtaccgcc acgctgcagc gatcgtcagt cagaggctgg ctttcggcat cttccgggtg
    43021 caaacgctgg cgaaaccagt catttatcgc cgcttcgcca aaatgaccaa acggcggatt
    43081 gccgatatcg tgcatcaggc atgacatctc aacaatgctt tcaaaaggtc cggtcagttc
    43141 atccaggccg tatgcttcca gcaatttaag ctctttcaga cggcttaaaa tttctttggc
    43201 gatgtagcgc cccacctgct ggacttccat cgagtgggta agacgcgtgc gcacggcggc
    43261 attgcgctcc agtggaaaaa cctgggtctt ttgttgcaga cgacgaattg ccggagagtt
    43321 gatgatacgc ccgcgatcgc tctcgaagat ccgcaggatc tcatgttcgg ttttaacgcc
    43381 ctgcggtgaa cggtaacgac gatgccagtt tatttttttt cggaaatcaa tctgtgccat
    43441 gtcctctccc gcgtgagaaa tacgcttccc cgtaagcgca tggtaaacta tgccttcaaa
    43501 tcgggcttat cgcgagtaaa tctatgaaaa tcggcatcat tggtgcaatg gaagaagaag
    43561 ttacgctgct gcgtgacaaa atcgaaaacc gtcaaactat cagtctgggc ggttgcgaaa
    43621 tctataccgg ccaactgaat ggaaccgagg ttgcgcttct gaaatcgggc atcggtaaag
    43681 tcgctgcggc gctgggtgcc actctgctgt tggaacactg caagccagat gtgattatta
    43741 acaccggttc tgccggtggc ctggcaccaa cgctgaaagt gggcgatatc gttgtctcgg
    43801 acgaagcgcg ttatcacgac gcggatgtca cggcatttgg ttatgaatac ggtcagttac
    43861 caggctgtcc ggcaggcttt aaagctgacg ataaactgat cgctgccgct gaggcctgca
    43921 ttgccgaact gaatcttaac gctgtacgtg gcctgattgt tagcggcgac gctttcatca
    43981 acggttctgt tggtctggcg aaaatccgcc acaacttccc acaggccatt gctgtagaga
    44041 tggaagcgac ggcaatcgcc catgtctgcc acaatttcaa cgtcccgttt gttgtcgtac
    44101 gcgccatctc cgacgtggcc gatcaacagt ctcatcttag cttcgatgag ttcctggctg
    44161 ttgccgctaa acagtccagc ctgatggttg agtcactagt gcagaaactg gcacatggct
    44221 aagtcactgt tcagggcgct ggtcgccctg tcttttcttg cgccactgtg gctcaacgcc
    44281 gcgccgcgcg tcatcacgct ttctcccgcc aacactgaac ttgcctttgc cgccgggatc
    44341 acgccggttg gggtcagcag ctattccgac tatcctccac aagcgcaaaa gattgagcag
    44401 gtttccacct ggcaggggat gaatctggaa cgcattgtcg cgctgaaacc cgatctggtg
    44461 attgcctggc gtggaggtaa tgccgagcgg caggttgacc agctggcttc gctgggaata
    44521 aaagtgatgt gggtcgatgc gacaaacatt gaacaaattg ccaatgcgtt acgtcaactg
    44581 gctccctgga gtccgcaacc ggataaggcc gaacaagccg cgcaatccct gctggatcaa
    44641 tacgcgcaat tgaaagcgca atatgctgat aaacctaaaa aacgtgtttt tctgcaattc
    44701 ggcattaatc cgccatttac cagtggaaaa gagtcgattc aaaaccaggt actcgaagtt
    44761 tgtggcggag aaaacatctt taaagacagc cgggttccct ggccgcaagt tagccgcgaa
    44821 caggtgttag cacgctcgcc acaggcgatt gtcattacag gcggaccgga ccaaattcct
    44881 aaaatcaaac aatactgggg tgaacagctc aaaattcccg ttattcctct cacgagtgac
    44941 tggtttgaac gtgcaagccc acgtattatc ctcgctgcac aacagctctg taatgcgctt
    45001 tcacaggtag attagcgcct gaccttttta ggtgctcagt ggggattgaa caatgctcgt
    45061 ctattggctg gatatagtcg gcacagcggt atttgccatc tccggcgttt tgttagccgg
    45121 aaaattgcgt atggaccctt ttggtgttct ggtactgggc gtggttaccg ctgtaggcgg
    45181 cgggacaatt cgcgacatgg cgctggatca cggcccggta ttttgggtga aagatcccac
    45241 cgatctggtc gttgcaatgg taaccagcat gctgaccatc gtgctggtgc gccagccaag
    45301 acacttacca aaatggatgt tgccggtgct ggacgccgtt ggtctggcgg tgtttgtcgg
    45361 cattggcgtg aataaagcct ttaatgcgga agccggtccg ttaatcgcgg tttgtatggg
    45421 cgtcattact ggcgttggcg gcgggatcat tcgtgatgtt ctggcccgcg aaatccccat
    45481 gattttacgt acagaaatct acgcaactgc ctgtattatc ggcggtattg tccacgctac
    45541 ggcttattac acattttccg taccactgga aacagccagt atgatgggca tggtcgtgac
    45601 gctattgatt cggctggcgg ctattcgttg gcatcttaag ctaccgacgt ttgcgctgga
    45661 tgagaatggg cgttgaagag agaagatgtg ccggatagtt tatccggcac atgaacaaca
    45721 gattagatac taaaggaaga accgcaaccg caggtgcttt tcgcgttcgg gttggtcacg
    45781 atgaaacgag aaccttccag tccttcggta tagtcaacgg aaccgccgac cagatattgc
    45841 aggctcatcg gatcaaccac caggccaacg ccctgttttt cgatggtcat atcgccttcg
    45901 ttcacctgat catcaaaggt gaaaccatac tggaagccgc tgcaaccgcc accggtgata
    45961 tacacgcgta atttcagatt cgggttatct tcgtcagcga tcaggctttt aactttgttg
    46021 gctgctgcgt cggtaaactc cagcggcagt gctacgtcat cactcatatt ttgctccaaa
    46081 cgacatcggc aattgggcaa attctaaccc aataataatc gccattatct aataccctgg
    46141 tatttcgttc aagtattctc gctggctgat gcggccttgc ttcgcgccag ttgctcagct
    46201 tcctgttttg ccagcgtgcg cgcaagaatc gccgagtata gcggtttccc gccggtaaat
    46261 tgcgctaata gtgttgcgcc aagaccggta ataatcattg gcaaaatgag ctggtagtta
    46321 tcggtcatct ccagaaccag aatgatcccc gttaacggcg cgcgaataga tgccgccagt
    46381 aacgccccca ttccggcaat agcaaacgtc cccgcctcaa ggtgatattg cggaaacagc
    46441 tcaacggcaa ccattccgaa agcggttccc agcacagtac ccagcgccag catcggggca
    46501 aaaataccgc ccggcgcgcc ggaagagaag cagagtaagg tggtaatgac ccgcgcgacg
    46561 aagataaaca ccagcattcc catgctgaaa ttccccgcgg tagcgatagg aatcaggtta
    46621 aaaccgccgc ccgacgttgc tggtgccaca aaccccagca atccacacag accgccaatc
    46681 gcaccaccca tcagtaccca tttggtaata ttgccgccgt gcacacggtg cagcaaatcc
    46741 tgcatcccca gcacccattt attaaaaata gggccgaaaa tgccaaaaat aataccgagg
    46801 atcagataaa gccacagcgt attaagcggc gcgtcagaaa gtttaccgac gtcaatcaac
    46861 gcaacttcat gattaaaaat ccggtacata atggtcgaca taatgacgcc aataaatacc
    46921 gctttaatcg aaattaacgt atagcgaaac tgcggacgca tctcttcgat aataaacaaa
    46981 atacccgcca gcggcgcgtt aaaggccgcc gccagacccg ccgccgcacc ggttgccaac
    47041 agcgtatggc gagcttcatc acctttcagg cggaaaacat cgagtaccat ccgcccgata
    47101 ttaccgccga tctgcacggt tggcccttcg cgccccaaca ccatgcctcc gccgagtgtc
    47161 cccagcccgc caaagaattt caccggcaat acacgccacc agcgaacagg acgttgatct
    47221 tccagcgccc cttcaatttc cgggatcccc gacccccctg cttcgggcgc gtatttgcgc
    47281 accaaaaagt agccgaacat cgccagcacc gccgaacaga gaaaagcaac ggttaacagt
    47341 agcggataat tatcagcagt atgtaccagc gcccccatac gttggttctg caaccaggcg
    47401 acacctttgt caaaagcaac cgctgccagc ccgacaagcg tgccgacgac tgccgccata
    47461 aacaaaatgg ctaacggggt tttatcgcgc tcaagaagtt ggcgaatcag ttgtctgcgt
    47521 cgcaggcgcg cggcctgcgg tgtttctaaa gagggagtat cagttttcat ccaatgatca
    47581 cttattggtc atacaaataa gatgacggca ttttactcgc acgttccatg aaaatccttt
    47641 gcaaagcgta atgtttcaaa tgcacaaaac ttttatactc taagaaatca ggattgcacc
    47701 agtacaagca gcctgatgtt tgacgagtat ttaacttgtt atgaataaca tagaatagca
    47761 gccattcact ttttctacga atcaggaacc ctccatgagt aagtctgaaa atctttacag
    47821 cgcagcgcgc gagctgatcc ctggcggtgt gaactcccct gttcgcgcct ttactggcgt
    47881 gggcggcact ccactgttta tcgaaaaagc ggacggcgct tatctgtacg atgttgatgg
    47941 caaagcctat atcgattatg tcggttcctg ggggccgatg gtgctgggcc ataaccatcc
    48001 ggcgatccgc aatgccgtga ttgaagccgc cgagcgtggt ttaagctttg gtgcaccaac
    48061 cgaaatggaa gtgaaaatgg cgcaactggt gaccgaactg gtcccgacca tggatatggt
    48121 gcgcatggtg aactccggca ctgaagcgac aatgagcgcc atccgcctgg cccgtggttt
    48181 taccggtcgc gacaaaatta ttaaatttga aggttgttac cacggtcacg ctgactgcct
    48241 gctggtgaaa gccggctctg gcgcactcac gttaggccag ccaaactcgc cgggcgttcc
    48301 ggcagatttc gccaaacata ccttaacctg tacttataac gatctggctt ctgtacgcgc
    48361 cgcgtttgag caatacccgc aagagattgc ctgtattatc gtcgagccgg tggcaggcaa
    48421 tatgaactgt gttccaccgc tgccagactt cctgccaggt ctgcgcgcgc tgtgcgacga
    48481 atttggcgcg ttgctgatca tcgatgaagt aatgaccggt ttccgcgtag cgctagctgg
    48541 cgcacaggat tattacggtg tggaaccgga tctcacttgc ctgggcaaaa tcatcggcgg
    48601 tggaatgccg gtaggcgcat tcggtggtcg tcgtgatgta atggatgcgc tggccccgac
    48661 gggtccggtc tatcaggcgg gtacgctttc cggtaacccg attgcgatgg cagccggttt
    48721 cgcctgtctg aatgaagtcg cgcagccggg cgttcacgaa acgctggatg agctgacaac
    48781 acgtctggca gaaggtctgc tggaagcggc agaagaagcc ggaattccgc tggtggtaaa
    48841 ccacgttggc ggcatgttcg gtattttctt taccgacgcc gagtccgtga cgtgctatca
    48901 ggatgtgatg gcctgtgacg tcgagcgttt taagcgtttc ttccatatga tgctggatga
    48961 aggtgtttac ctggcaccgt cagcgtttga agcgggcttt atgtctgtgg cgcacagcat
    49021 ggaagatatc aataacacca tcgatgctgc acgtcgggtg tttgcgaagt tgtgatgatt
    49081 ttgcctcgcc caccgggcga ggcattttat taacggctct gctttctcaa caaatagata
    49141 aaatatggcg cgccgataaa ggtcgacagc agccccgccg ggatctggaa cggaaacagc
    49201 accatccgcc cacaccagtc agcgaacacc agcagtaaac cacccaccag cgccgaaatc
    49261 acgatgtgcg gcatcgtccg tcgaaagccc atcatccgcg cgatatgcgg tgccattaaa
    49321 ccaacaaaac tcaacggtcc aatggtcatc gtcgcggtcg ccgtcaggca agccgctaac
    49381 agcagcagcg caattcgcgt cggcgttaac gcccccccca cggcccgcgc ggtatcacca
    49441 cccagcggta aaatggtcag ccagcggcgg cacagcgggg taatcgccag caaaatcacc
    49501 atcacaattc cggtgcgcca gacctgcgca tcggtggcgt tgtaggtcga accagagatc
    49561 caggtcagca cttgcgccat tcgcgggtca ccactcgcct gcaacatcat taaaagcatg
    49621 gtgaatgcgg tacttaacgc catccccgcc agtaacatgc ggtgtgggga aaatccaccg
    49681 cgcccggcgg cgatcataat aatcaacagc gtcaccgccg caccgatact tcccgcgggt
    49741 aacagccagc caaaagcatt acccggtacc agaaacagca tcagtaccac gccaaacgcc
    49801 gcgccagagc taatccccag cacttccggg cttgccatcg ggtttccggt cagccgctga
    49861 ataatacagc ccgccaccgc cagcatgacg cccgcaaaca gcgccgccat aattcgcggc
    49921 cagcgccagg gcatcaaatc ctcgagcaac gccccgctcg cccacgtcca gccgtgcgca
    49981 tcacgaccaa acgacagcgc caccaccaca gccatcaaca gcagcacgcc gcccgcgagg
    50041 gcaaacgcca gcacatgttg gcgttcagcc gcgacacgat cgttgacctt catatccggc
    50101 gcgctaatgc tgcgtaaacg cggcaacagc cacagtagca gcggcgcacc gatcaacgca
    50161 gtgaccgaac cggtggacac ttccatccac acgcgagtca gccagaggat gatttgatcg
    50221 gaaagccaga ggatcagcgc accaatcaac gacgccagca tcagtcgcgg cagcagacgc
    50281 cgcgccccca gcatttttgc cagcaacggc gcgaacaacc cgataaagcc gataatcccc
    50341 accgcgttca ccagcagcgc actgatgaca atagccagcg acagcgccgc cagacgcgcc
    50401 agcgacaagg ctagcccgag attgcgcgcc acgccatcat caagccccat cagtgttaac
    50461 ggacgaagta gcagcaacgt cagcatcaca ccgcccagca actgcggcca taaacgctca
    50521 acaccgcccc agtcggtttg cgtcagcgtt ccggtgctcc acaggaacat gctttgcagt
    50581 tggtcatggt ggaagataac cagtaactga ttgattgccc cacaataaag gctcactacc
    50641 agccccgcga ggatcagcgt taccggcgaa agccgtttcc cccacgcgac gccaaagacg
    50701 attaagccaa caacgcaagc gcccgccagc gcagcaaact gactcgccat cgcccctggg
    50761 atcgcccaga gcgtagtgac ggtaatcccc agttgcgcgc ctgtagcaac gccaagtgtc
    50821 gtcggttccg ccagcggatt acgcagcact tgctgaaaca gcacgcccac cagccccagg
    50881 cctgcgccca ccagcagcga aatcgccaga cgcggcagca agctgtagtg aaaaatcatc
    50941 tgctcgatga cgtcaatatc cggcgaccag gcagcctgcg cccactggct acgcggcagc
    51001 gcctgcgaga agttcatcca ggtcagcgcc gtagcggcaa tcaccagcaa cgccagcaat
    51061 aatgccggga aaagcgcaat acgtttactc acgctttacc tccaatggcg ttatccagaa
    51121 cgcgcacaaa gtgcattgcc gagagagttg caccataaaa ccagactgcg ggtacgcgct
    51181 gaaagcgtcc ggcgcggaca aacggcatgg cctgccacag cggcgttgcc attagcgcat
    51241 ccatgtcttt gctgttgtcg tgatcaaaac agagcacatc aacgtcttta tacgctgcca
    51301 gacgatcgat gctgacggcg gtactgcccc agaagttggt ttccccttgc caggcatttg
    51361 ggatgccgta ctcatcaaga atttcctgga acaagctgtt tggaccgaag accagcatat
    51421 ggcgcggatc gataagcgtc gtcagcaata acggacgcgc accacgcttc acaaagcggg
    51481 ggttcatgct gcggataaag tcttcatatt gcgctaaatg cgtttctgct gcgctttgca
    51541 ggttaagtaa atccgccatt tccgtcagcg atttacgcgc catcgccagc ggctgtttgc
    51601 cgtcgctgaa gttaaatcca cgccccggcg caatacgagc cagcatttct ggcgaaggac
    51661 catatcccgc cgaccagacc ataaacgacg gtttcatttc ggtcagcagt tcgaggttag
    51721 gttctgtacg caaaccgacg tcaatcaccg attcaggtag tggtggttcg ctgacccaca
    51781 gtcgatagtt gatggtgtct gccacgccat aaggcacgat gccgagcgcc agtagtaatt
    51841 ccaccggcag ccactccagc gccacaatac gatttgggtc aatagccgcc gcgtgggcgg
    51901 tattcatctg ccataacaac ggagaaagcg ccatcgccgt taacagtcga cggcgcgaaa
    51961 taagaggtaa gccgctcatc aataaacaaa actcacaggt gcagcacccg ccggatgcgg
    52021 cagaataccc atcgggatgc cataaatcat ttcgagggtt tcgccgcgca taatttccgc
    52081 aggcgttccc tgagcaatca tttcaccgcc gcgcagggcg accagataat cacagtaacg
    52141 cgcagccata ttgatatcgt gcagcacggc aatgaccgtc aggccacgct cctgacttaa
    52201 acggtgcacc agcgccagca catcaacctg gtgggcgata tccaacgccg aagtcggttc
    52261 gtcgagcaac agacaacggc tatcctgcgc caccagcatg gcgatccacg cccgctgacg
    52321 ttcgccgcca gagaggctgt ctaccagccg atgcgccagc ggttttaagc caaccagcga
    52381 gatagcttcc tcaacttttt cgcgatctgc cgcaccaaag cgccctagtg cgccatgcca
    52441 cgggtaacga ccaatcgcca ccagttcacg cacggtcatc ccttctgccg gaggaagctg
    52501 ctgcggcaaa taagccactt tgcgggcgaa cgctttgctg ctccagcttt ccagcggctg
    52561 ggcatcaaga agaatctccc cttccgacgg cggctgatga cggccaagca ttttgagcag
    52621 agtggattta ccagaaccgt tgtgaccaat cagaccggtc actttcccgg caggaaaggt
    52681 taacgacagc ggatgcaaaa gcgtgcgccc gggcacacga aaggagatat tacgcagtgc
    52741 aaaagtggta tcggaatgat tcgtgtattc ctgcataaca accaacttgt gaaacaggca
    52801 cggaaatccg tgccccaaaa gagaaattag aaacggaagg ttgcggttgc aacgacctga
    52861 cgttctgcgc cccagaagca gccataagtg ttaaagcagc tggcgacgta ttcacgatcg
    52921 aacaggttgt taacatgcag cgccacgttg gagcccgcca tgccgactcg cgccagatca
    52981 taacgcacta acgcatccac gaccgtataa cttcccactt taaaggagtt agccggatca
    53041 ccatagctgg agccagtata acgaccaccg gtgcccagcg tcagacctga aagcggaccg
    53101 tcaaagaagg tatagtcagc ccacagcgaa gccatgtgtt ttggcacctg tgcaggcgta
    53161 ttgcctttat aggtagtatc ggtggtgtat tccgcatcgg tgtaagtata agaaccgact
    53221 acgttaacac tcgccgacag cgccgctttc gcttcgattt ctacgccacg tgcgcggatc
    53281 tcgccacctt caaccgagaa gaaggaaccc tcagggtccg ccatcaggtt gttggtttta
    53341 gtgagattat acacggcacc agtaactaca atcggacgat cttccggtac atatttcacg
    53401 ccgacctcat actgcttacc tttagacggt gcgaaaatat taccatcctt cccaacttgc
    53461 gaagaaggtt caaacgattc gctatagcta aagtaaggtg ttacgccatt atcaaacagg
    53521 tagttaacac caccacgcca ggtaaactgt ttgtcatcac gtttatcggt cgtcccggca
    53581 acgcggttaa gagattcttg atctgcccag tcataacgac cgcccagggt gaccagcact
    53641 ttatcccact gcgcctgatc ctgaacataa acgcccgttt gtttctgctt attcagaatg
    53701 cggtaagggc cggagtttgc cggatctttg gcattgaagt cgaaatcggt attcaccgga
    53761 ttgtacagat cgagcagcgg tacggagtcg tcgtaaccaa accaggcgtt gatgtcatta
    53821 cgcatacgca taaagtcgac accggtcagc agggtgtggt cgatatcgcc agtggcaaac
    53881 ttgctctgca actgggtatc aacggagaag ttttgcagct tctcatcatc aacgacgtat
    53941 ttacgtgcca gataatggcc tttatccgct ggcgctaatg ccgcacactg tttgctgtaa
    54001 gcattcgccg gatcggagca gacgccgtaa ccataaacgc tgttttgcga ggttttgttt
    54061 tcagcaaagc gcaggttctg acgcacagta aaggtgtcgt taaattcgtg atcgaagctg
    54121 tagcccacca tcttctcatt acgagaatag gtgttgttct tcgccccttc gttaaagtct
    54181 gtcggcagac gcttaccgtt cggcagcggc tcaacggttc cctctttcgg caaccagccg
    54241 taataaccgg tttccggctc gttctggaag taagaaagga aggtgaaatt ggttttatca
    54301 tccggacgcc aggtgaacgc cggtgcaata gcataacgct gctcttctga ccctttctgc
    54361 tgggcattgg cagaacgcgc aagaccggtc aggcgataag agtaaacgcc gtcatcatcc
    54421 agcgcatcgc taaagtcaaa accagtctgg aacaggctgt cagtaccggc tttaaactga
    54481 acttctttca gcggttcggt ggtcggacgc ttgctgacca tattcaacag gccgccagga
    54541 ctgcttttac cgtaaagcac ggaaaccggg ccacgcataa tttcagcgcg ttccagcata
    54601 tacggatcaa tgaccgcatc gttatagaag ttgccctgca acttcaggcc attcagatag
    54661 ttattctggc tttggccttc tgccgcaaaa ccgcgaatga tcaggtggtc ataggtgttg
    54721 gatgcgccac gcgtaccaac agagacaccc ggcgtgtagc taagcgcttc ttttaccgac
    54781 ttcggctgat gcagcgccat ctcttcggcg gtcacaacag aaatagactg tggcactttt
    54841 tgaatcggcg tatcggtttt agtgccggta gctgactgtc gcgccgcaat agttgcagca
    54901 ggcccccatg cgctttcttg cggcgcaggt gcagcggtaa cggtgatagt gtcttctttc
    54961 ggttcaaccg ctgcctgtgc ataaacagac atgccgctaa ccgctgtggc tactacaact
    55021 gcgattttac gcagtgagtg ttttggctga gcagttttgg aacgcgccat tggtatatct
    55081 ctgatgtaaa gtgaatgata acgtaaacga gaatgattat tataaagata agataatatt
    55141 cgaaactctg gcgggttcgc aagcggtacg caatctgagc gttcctgaag ggttaataaa
    55201 caaccagatg aaaagaaagg gttaatactt tgggtgggat gttattttac cggatggcaa
    55261 ctcgccatcc ggtatttcac gcttagatgt taattactac caaacatatc cttaatccaa
    55321 ccggctacac cgtcgctgtc tttctgctct tgctgagcag gttgctgttg cggctgttgc
    55381 tgcggctgag aagactgatc aaacggattg cctgacggct gctgctgcat ctcactctgc
    55441 tggcacagcg attgcggatc gctggtccag accggcaaga cacgcatgcc accgctgcaa
    55501 acaaagttgc cgtcgtagtc cacgcccata tctgcaatat cttctggcgg aacaagattc
    55561 agcggcgttg gcgtctggtt agccagataa cgctgataaa tcgacattgc cccgctggct
    55621 ccatacagtt tggtcggctg gttgttatca cggccgaccc aggtgatggt caccgtgctg
    55681 ccgtcaatgc ccgcaaacca ggtatctacg ttattgttgg tagtccctgt tttccctgcc
    55741 agatgcaggt tcgggtattt cgccccaagc tgacgacccg taccgcgttg taccacctgc
    55801 tgcatggtcc atagtgtcag atacgccgcc tgcgccggaa cagcgcgttc cgcctgcggg
    55861 aagctctgat acagcacttt gccatcttcc gcgattaccg aacgcagcgc agaaagcggt
    55921 gcacggttac caccgctggc gatggtctgg aatgcctgcg ccacttcgat tggcgttaag
    55981 ttcaacgccc ccagcagcat tgccggaacc ggatgcaact gatctttcgg tacgcccagt
    56041 ttaatccagg tatccgtaac cgcaggcagc cccagcgcca tccccagatt taccgtcggc
    56101 acgttcatcg aacgggtcaa cgcatccacc agcatcactc tgccgctttc gctataacga
    56161 cggtcatcat tctgcggtga ccagacctgg ccattcggct gacgcagcgc aattggcgca
    56221 tccgcaatcc acgtattcag acgatagatt ttcggctggc ttaaggccgt cagataagtc
    56281 gctggttttg caagggaacc aatcgaacga cgcgcctgca tcgcacggtt gtagcccgca
    56341 aactgcggct cagaacctcc gaccatcgca cgaacttcac cactaaagcg gtcgacgacc
    56401 acaatcgcag tttcaagatc gctcaactta cgctgtttct tcagtgccgg aataccttcc
    56461 acggcggctt tttctgccgc gtcctgggcc accgagtcaa aggtagtgaa gatcttcacg
    56521 ccggagagat cttttacctt atcgcctaat ttagcctgaa gctcctgacg caccagttgc
    56581 ataaaggctg gctgaggaga gatcacccca ccgcgcggct gaacccccag cggacgcgca
    56641 ctcaacatgt catagagttc ttgatcaata atctgttgct gttgcagcag acgcagtacc
    56701 agattacgtc gctccagcgc cagtttcggg ttacgccacg ggttgtagat ggacgctcct
    56761 ttcaccatac cgactaacag cgcctgctga tcgaggctca attcttctac cgggcgacca
    56821 aagtaataca agctcgccag cgggaagccg cggatttcgt tgtcgccgct ctgaccgaga
    56881 tacacctcgt tcatatacag ctcaagaata cggtctttgc tgtaacgcgc gtccatgatc
    56941 agcgccatgt aggcttcatt cgctttacgc caataagagc gctcgctgga gaggaacagg
    57001 tttttcacca gctgttgcgt cagcgtactc gccccctgca ccgtgcgacc tgcggtcagg
    57061 tttgccagca ccgcacgtcc gatggagtag agactgattc catcatgctc gtagaaatgg
    57121 cggtcttctg tcgccagcaa agtatccacc agcaaatccg ggaaaccact gcgcggcaca
    57181 aacaaacgct gctcaccgtt tggtgaggag atcatggtga tcagacgcgg atcaagacgg
    57241 aagaaaccga actgacggtt gttctccata ttgacgatgg tcgccagatg atcgccatca
    57301 aaggtcagac gcgcgcgcac ctgcccttct ttactgtccg ggaaatcaaa cggacggcga
    57361 atcatctcaa tgctgttggc ctgcacggta aattcgccag gacgggtcat tttcgacacc
    57421 tgacgatact gtgttgcctc cagcagcttc accatctcgt tcttgctgat ggtcatgtct
    57481 ggctcaaggt tgaccattcg accataaact gccgcaggca gttgccagac cttgccatca
    57541 atacggctac gaattttttg atcgagataa acgccgtaaa tggcgatcag cacggcaaaa
    57601 acgatagcca gttttaacag cagccatagc cagccgcgtt tgccacgagg cttacgccct
    57661 ttgcctttgc ccttaccttt gcgcggcatc ggttcttcat cctcatagtc atcataatcg
    57721 tcgtaatcat cgtcatcttc gtaacgacga cggcttacct tttgtttgac cggacgcgtc
    57781 ggtttccctt tgcgtccaat tggctcgcgg tcattcccgg ccatgctttt tctccgcaat
    57841 attcaggcgc aaaagcccga ttttctgttc ttctgttaca agacagaaga agatatctct
    57901 caaaatttga gctcgatcgg tcccctctcc cttaagggag agggttaggg tgagggtgaa
    57961 cgtgcgcgct ggtgccctca ccccggccct ctcccacagg gagagggaga aaaccaacct
    58021 tacgaatact ttttcgtccg tcgcgtcggt gccgtgtttg ccgggtcgtc cggccagacg
    58081 tgtttgggat aacgcccttt catctccttt tgcacctcac gatacgcccc tttccagaag
    58141 gcccccaaat cacgcgtgat ttgcagcggc ctttgggcgg gtgaaagcaa ttccagcacc
    58201 agcggcacgc gcccctgggc gatcgtcgga ttagtggcct caccaaacat ctcctgcatt
    58261 ctcaccgcca gcgcgggcgg gttatcttca tgataacgaa tggcgatccg gcttcccgtc
    58321 ggcacagtgt aatgcgcagg caattcacta tccagacgtt gctgcattcc ccaatcaagt
    58381 aatccgcgta gtgcctgata aatgtcgagt gatttcaggc cgcgtagtga atgtacaccg
    58441 gccatatgtg gcagcagcca cgtttccagc gtcgccaaca aactttcatc atcaaccgct
    58501 ggccagtcat attctggcaa ccactttccg gcgcataaca aacgcaagcg tagctgttcc
    58561 gcttccgccg tccagttgag cacgcttaaa cctttatcac ggatgccgtt aagcatcgcc
    58621 tgatgcaact cgtcttctga cggtttcgcc aacggctgca ctttcaccgt caactgaccg
    58681 atttgcagcc gacgccaggc tttcagcgta ccttgcgcgt catcccattc cacggtgtca
    58741 gattgctgca ccagctgcgg acagcgttgt actaattcat caatatcgac cggtaacgcc
    58801 agtaaaatcc gcgcatccgg cgaggcgctg ccctgcaata ataacggcgc gatcaaccat
    58861 tcgtggcgac ttagcgcgtc gtcggcatca agcatcgccc ccatgccgtt tgccagttga
    58921 tagcgtccat cttgcccacg gcggcgagca atgcgatcgg caaacgcccc ggcaagtagc
    58981 ggcgcgataa gcgaactgtc tgcctcaccg ccgcgtacgt ttaagcgttt taacagttgc
    59041 tgactacgtt gctgccaggc tggttgattg cgcgaaaacg ccacgcccag gtcactattg
    59101 cccatccgtg gcggctcttc gagaatggcg gcaatttttg ccgcggtagc agcttcgtcg
    59161 tcgctctttg cgctaaccag cattgccgct aaacgcggat cgttacccag cgctgccatt
    59221 ttttgccctt gcgcactaag ccgttcaccg tccagcgccc ccagcatttg taacagacgt
    59281 ttcgcggcca gtagattcac cgttggcggt tgatccagcc agctcatctg cgccggatcg
    59341 ctgcatcccc attgcagtaa ttccatcagc aaaccggaaa gatcgctttg taagatctcc
    59401 ggttcacttt gcgcggtagc gcgttctgct tgttctttgg cgattaaatg caggcagata
    59461 cccggctcca gacgcccggc gcgcccggca cgctgcgtca tcgacgcctg actgattcgc
    59521 tgagtaatca accgcgtaag ccctgttcgc ggatcaaaac gcgccaccct ttcctgggca
    59581 caatccacca ccagacgaat accttcaatg gttaaactgg tttcagcaat attggtcgcc
    59641 agcaccactt tgcgcatccc ttgcggtgcc gggaggatcg cttttcgctg atcgttcagc
    59701 gacaacgcgc catacagcgg gcagagcaat acatcactgc cgatgcgcga agccagttgt
    59761 tcctgcacac gctgaatttc tccgacgcca ggtaaaaata acagtaatga tccgctttcc
    59821 tgacgcagca tttcagcggt ggctaccgca acggcatcgt caaaacgctg atgcgtgggc
    59881 agcggtaaat aacggcgttc aaccggaaac gagcgccctt ctgagatgac gacgggcgct
    59941 tctggcagca tttgctgcaa gcggtcgttg tccagcgtag ccgacataat cagcagttta
    60001 aggtcatcac gcagaccttg ttgcacatcg agtaacagcg ccaacgccaa atccgcctgc
    60061 aagctacgct catgaaattc atcaaggatc accaatccaa caccgctcag ttccgggtca
    60121 cgctggatca tccgcgtcag cacgccttcg gtaactactt ccaggcgggt attcggcccg
    60181 acgcagtttt gcgcacgcat ccggtagcca acggtatcgc ctggcttttc gttaagcagc
    60241 tccgccagcc gttgcgcgac gttacgcgcc gccagacgcc gtggctccag gaggataatt
    60301 ttcccgttaa tgccgggatg cgccagcagt tgcagcggca gccaggttga tttcccggcc
    60361 ccggtcggcg cacttaataa tacctgcggc gcataatcga gagcggtaag taattcaggt
    60421 aacacggcag caacgggcaa cgacgacaca aaacgctcca gagggttaac attcttcgcg
    60481 ccacattgta gcatcgcggt aattcataac cgagtgcctc acatgtctga cccgcaacgt
    60541 ctgttctttg ctatcgactt acctgcagaa atccgcgaac agattatcca ctggcgcgcc
    60601 acacacttcc cacttgaggc gggacgtccg gtcgccgccg ataatttgca tctgactctg
    60661 gcatttttag gcgaagtgag cgcagagaaa gagaaggcgc tttctctttt agccggacgg
    60721 attcgtcaac ctggtttcac actcacgctt gatgacgccg gccaatggct gcgttcgcgt
    60781 gtggtgtggt tagggatgcg tcagccgcca cgcggcttaa tccagctggc gaatatgctc
    60841 cgttcacagg ctgcccgcag cggttgtttt caaagcaatc gtccgtttca tccacatatt
    60901 accttattgc gcgacgccag cgaggcggtg acaatcccgc cgccaggttt taactggtca
    60961 tatgcggtga cggagttcac cctttacgcc tcctcgtttg cccgtggacg cacacgctac
    61021 acgccgctaa aacgctgggt gctaacgcaa taacaaggat tgtcgcaatg gaattttctc
    61081 cccctctaca gcgcgcgacg ctaattcagc gttacaaacg ttttttagcc gatgtgatca
    61141 cacccgatgg tcgcgaatta acgctacact gcccgaatac gggtgcgatg accggttgtg
    61201 caacgcctgg cgataccgtc tggtattcga cttcagacaa caccaaacgg aaatacccac
    61261 acacctggga attaactcaa agccagagcg gcgcatttat ttgcgtcaac acgctttggg
    61321 ctaacaggtt gacgaaagag gctatcctta atgaatcaat ttcagaactg tcaggctata
    61381 gctcgctgaa aagcgaagta aaatacggcg cagaacgcag ccgtattgac tttatgttgc
    61441 aggcggattc gcgtccagac tgctatattg aagtgaaatc ggttacgtta gcggagaacg
    61501 aacagggata ttttcccgat gcggtcactg aacgaggtca gaaacacctt cgggagttga
    61561 tgagcgtagc ggctgaaggc cagcgtgcgg ttatcttttt cgccgtgctg cattcagcca
    61621 ttacacggtt ttcacccgcg cgccacatcg atgagaaata cgcgcaacta ttgtcagaag
    61681 ctcaacagag gggggtagaa attctggctt acaaagcgga aatttctgct gaaggcatgg
    61741 ctcttaaaaa atcactgccg gttacattgt agtaaagtaa gtaactggtt aatttacatt
    61801 ctggtcgcgt gcgcaaatac gcttttcctc acacagttgt caagtgttac gtttagataa
    61861 ttgctatccg gaaaagcatc tgctatttat agcggcctca tttttccccc gaacatgggg
    61921 atcgatagtg cgtgttaagg agaagcaaca tgcaagaagg gcaaaaccgt aaaacatcgt
    61981 ccctgagtat tctcgccatc gctggggtgg aaccatatca ggagaagccg ggcgaagagt
    62041 atatgaatga agcccagctg gcgcacttcc gtcgtattct ggaagcatgg cgtaatcaac
    62101 tcagggatga agtcgatcgc accgttacac atatgcagga tgaagcagcc aatttcccgg
    62161 acccggtaga ccgtgcagcc caggaagaag agttcagcct cgaactgcgt aaccgcgatc
    62221 gcgagcgtaa gctgatcaaa aagatcgaga agacgctgaa aaaagtggaa gacgaagatt
    62281 tcggctactg tgaatcctgc ggtgttgaaa ttggtattcg ccgtctggaa gcgcgcccga
    62341 cagccgatct gtgcatcgac tgcaaaacgc tggctgaaat tcgcgaaaaa cagatggctg
    62401 gctaattaca gccgttccat cacgtttacc acaggcggga aatgctcccg ccttattttt
    62461 tgttcaaaga gatgacagac acacagtata ttggccgctt cgccccctct ccttccggcg
    62521 agcttcattt tggttctctg attgctgcgc ttggcagcta tttgcaggct cgcgcccggc
    62581 acggtcgctg gctggtacgc attgaagata tcgacccgcc tcgtgaagtt cccggtgccg
    62641 cagaaactat cctgcgccag ctggaacatt acggtctgca ctgggacggc gatgttctct
    62701 ggcaatcgca acgtcaccac gcctatcgtg aagcactcgc ctggttacat gaacaaggac
    62761 taagttatta ctgcacctgc acgcgtgcgc gtattcaaag cattggcggt atttacgacg
    62821 gtcattgccg ggtgttgcat catggaccag acaacgccgc agtgcgtatc cgccagcagc
    62881 atccggtcac gcaatttact gaccagctgc gcggcattat tcacgccgac gaaaaactgg
    62941 cacgggaaga ttttattatt catcgccgtg atggtttgtt tgcctataac ctggcggtgg
    63001 tggtggatga tcacttccag ggcgtgagcg aaattgtccg cggggcagat ttgatcgaac
    63061 cgacagtgcg gcaaatctcg ctgtaccagc tttttggctg gaaagtgcca gattacattc
    63121 atctgccgct ggcgcttaat ccacaaggcg ctaaactttc caagcagaat catgcgcctg
    63181 cgttgccgaa aggcgatcca cgcccggtac taatcgcggc acttcaattt ctggggcagc
    63241 aggcagaagc acactggcag gatttcagcg tcgagcaaat ccttcagtca gccgtcaaaa
    63301 actggcggct taccgcagtg cctgagtcgg caattgtaaa ttcaacattc tcaaatgcgt
    63361 catgctgagc tatgattagc cgctattttt ttgtcctgaa tgatgtttga cactaccgag
    63421 gtgtactatt tttacccgag tcgctaattt ttgccgcaag gtgctaagcc gcgaggaaag
    63481 cgaggctgaa caggcagtcg cccgtccaca ggtgacggtg atcccgcgtg agcagcatgc
    63541 tatttcccgc aaagatatca gtgaaaatgc cctgaaggta atgtacaggc tcaataaagc
    63601 gggatacgaa gcctggctgg ttggcggcgg cgtgcgcgac ctgttactcg gcaaaaagcc
    63661 gaaagatttt gacgtaacca ctaacgccac gcctgagcag gtgcgcaaac tgttccgtaa
    63721 ctgccgcctg gtgggtcgcc gtttccgtct ggctcatgtg atgtttggcc cggagattat
    63781 cgaagttgcg accttccgtg gacaccacga aggtaacgtc agcgaccgca cgacctccca
    63841 acgcgggcaa aacggcatgt tgctgcgcga caacattttc ggctccatcg aagaagacgc
    63901 ccagcgccgc gatttcacta tcaacagcct gtattacagc gtagcggatt ttaccgtccg
    63961 tgattacgtt ggcggtatga aggatctgaa ggacggtgtt atccgtctga ttggtaaccc
    64021 ggaaacgcgc taccgtgaag atccggtacg tatgctgcgc gcagtacgtt ttgccgccaa
    64081 attgggtatg cgcatcagcc cggaaaccgc agaaccgatc cctcgcctcg ctaccctgct
    64141 gaacgatatc ccaccggcac gcctgtttga agaatcgctt aaactgctac aagcgggcta
    64201 cggttacgaa acctataagc tgttgtgtga atatcatctg ttccagccgc tgttcccgac
    64261 cattacccgc tacttcacgg aaaatggcga cagcccgatg gagcggatca ttgaacaggt
    64321 gctgaagaat accgatacgc gtatccataa cgatatgcgc gtgaacccgg cgttcctgtt
    64381 tgccgccatg ttctggtacc cactgctgga gacggcacag aagatcgccc aggaaagcgg
    64441 cctgacctat cacgacgctt tcgcgctggc gatgaacgac gtgctggacg aagcctgccg
    64501 ttcactggca atcccgaaac gtctgacgac gttaacccgc gatatctggc agttgcagtt
    64561 gcgtatgtcc cgacgtcagg gtaaacgcgc atggaaattg ctggagcatc ctaagttccg
    64621 tgcggcttat gacctgttgg ccttgcgagc tgaagttgag cgtaacgctg aactgcagcg
    64681 tctggtgaaa tggtggggtg agttccaggt ttccgcgcca ccagaccaaa aagggatgct
    64741 caacgagctg gatgaagaac cgtcaccgcg tcgtcgtact cgtcgtccac gcaaacgcgc
    64801 accacgtcgt gagggtaccg catgacagtg gcgtatattg ccataggcag caatctggcc
    64861 tctccgctgg agcaggtcaa tgctgccctg aaagcattag gcgatatccc tgaaagccac
    64921 attcttaccg tttcttcgtt ttaccgcacc ccaccgctgg ggccgcaaga tcaacccgat
    64981 tacttaaacg cagccgtggc gctggaaaca acacttgcgc ctgaagaatt acttaatcac
    65041 acacagcaaa tagagttgca gcaaggtcgc gttcgtaaag cagaacgctg gggaccgcgc
    65101 acactcgatc ttgatatcat gttgtttggt aatgaaacca ttaataccga acgactcacc
    65161 gtcccgcatt acgatatgaa gaatcgcggc tttatgctgt ggccgctgtt tgagattgcc
    65221 ccggaactcg tgtttcctga tggggagaca ttacgtgaaa tactcacttc gctagcagta
    65281 ctacaacctg ccatctggta ttagctgtat tattttattt tcttatctta ctcctcactt
    65341 cccctcttac agattaaata tacttaattt acattttagt tttttttaat taattcatga
    65401 aatagcactt catgcaaata gattaggcat tttttgttta aaaaacctag aatagcaaca
    65461 attcaataca ccacgtaaat atattcacac ccgtgataat tcacccctat tacatcctcc
    65521 aaaaactttg agtaatatcc gctcgcaata ttaatttcat ataatttttg taaggatgca
    65581 tgtaatgaaa aaagcacttc tcgcagccgc tctggttatg gcttctggtt ccgccctggc
    65641 tgtagatggt ggtcatatcg actttaacgg tatggtacag tccggtacct gtaaagtggg
    65701 tgtggtagat actggtatgc atagcgttac cactgatggc gtggttaccc tggatactgc
    65761 gaatgttact gatacttttg ctgaagttag cgcaactgct gtcggtttac tgccgaaaga
    65821 gttcatgatt tctgttgagt gtgatccagg tgctccgaag aatgctgagt taactatggg
    65881 ttctgcaagt tacgcgaaca ccagcggtac cctgaataac aatatgaaca tcactgttaa
    65941 cggtattgca ccggctcaga acgtaaacat tgcagttcat aacatgaaaa acaaagctgg
    66001 cgctgctgaa attaagcagg tccatatgaa caactcttct gaagttcagg aactgacatt
    66061 agacgcagaa ggtaaaggcc agtacgtatt taacgcatct tacgttaaag caccgaacag
    66121 cccggctgta actgctggtc atgtaaccac taacgcgctg tacaccgttg cttataagta
    66181 attgatggtg tgttgagcat aaggtttgcc agcaggcaaa ccttattttc gcgaatttaa
    66241 tctttctaca gacagcaagg agtgcttatg tttcccttgg ttaaaaaaac ggttagtgca
    66301 ttatttgtga gtactctgtt agccagtgca ccagcattcg ctgacattat tatttccgga
    66361 acacgtatca tttataacgc tgataaaaaa gatgttaacg ttcgtctgga aaataaaggg
    66421 aatcgcccct tactgattca gaactggttg gatacgggcg atgacaatgc cgatcccagc
    66481 cagatcaaag tgccgtttac cgcgacccca ccagtttcac gcgtggagcc aaaacgcggt
    66541 caaaccgtaa aagtgatgta taccggtgca acagccctgc cgaaagaccg tgaaagcgtc
    66601 tactggttta acgtgctgga agtgccgcct aagccgaaag atgctgaggc tgacaaaaac
    66661 ttgttacagc tggctttccg cacacgcatc aaactctttt atcgcccgag tgggttacag
    66721 ggagaaccgg ctgaagcacc ggcgaaaatc acctggaaat taaacaacgc tcagttacag
    66781 gcaaataacc caacgccata ttacgtgtcc tttaatgagg tcaagctgga aagcggcggc
    66841 aaaacctaca acgtcaattc cagcatggtc acgccgttca gccaggcaag ctttggcgta
    66901 accagtctgc caggaagcgt gtcatccgga aaagtggtct ttaaagcgat caacgacttt
    66961 ggcggcaaca ttgatggttc cgcaacattc taaaaaataa acggattctt tttaacatcg
    67021 ccaggaatgc aaccttgtac cagtttactc atcaaaaaag ccgtatcccg aaaaaaacgc
    67081 tacttgcggc ctgttgtgcc ctgttttata gcagcaacgg tgctgcggcg gacaccgtgg
    67141 aatatgacag ttccttttta atgggaactg gcgcatcaac gattgatgtt aaacgttatg
    67201 ctcaaggcaa cccgacaccg ccgggtctct ataatgtccg cgtatttgta aacggtcagg
    67261 cgacttccag cttagaaatt ccgtttgtgg atattggcga aaacagtgcg gcggcctgtc
    67321 ttacccataa aaacctggcg caacttcaca ttaagcaacc tgaacagcct gtcactttac
    67381 tcgccagaga aggtgaagaa gaggattgtc tggatctggc aaagtcatac gaaaaggcgg
    67441 atgtgtgctt tgacggtagt gaccagtttc tcgatctgac gatccctcag gcctatgttc
    67501 tgaaaagcta tggcggctac gttgaccctt ctttatggga atcgggaatt aacgctgcca
    67561 cactggcata taccctgaac gcgtatcaca caagttcaga taacgacaat agtgacagcg
    67621 tctatggcgc gttcaactca ggtatcaatt taggagcctg gcactttcgt gcgcgcggta
    67681 actataactg gacaacagat aacggcagcg atttcgattt ccaggatcgt tacttacagc
    67741 gtgacattcc ggcaatccgt tcccagataa ttatgggtga tgcctatacc accggtgaaa
    67801 cgtttgactc tgtcaacgtc cgtggtgttc gcctgtacag cgacagccgt atgctgcctt
    67861 cggcgctggc cagttacgct ccgaccatcc gcggtgtagc aaactccaac gccaaagtca
    67921 ccgtgacgca aagcggatat aaaatttatg aaaccaccgt tccgcccggt gaatttgtta
    67981 tagacgacat tagcccttcc ggctttggta gcgaactggt cgtgaccatt gaagaagcgg
    68041 atggttccaa acgcaccttt acgcaaccct tctcgtcggt tgtacaaatg caacgtcctg
    68101 gtgtgggccg ttgggatttc agcgcgggta aagtcattga tgacagtctg cgatccgaac
    68161 ccaatatggg gcaagcctct tattactatg gtctgaataa cctcttcacg ggttataccg
    68221 gcattcagtt caccgataat aactatcttg ccgggctgtt aggtgtgggt atcaacacca
    68281 gcatcggcgc ctttgcggta gacgttaccc attcccgtgc tgaaattccg gatgataaaa
    68341 cctaccaggg gcaaagttat cgcgtgacct ggaacaaact tttccaggat accgggacat
    68401 catttaacct cgcggcgtac cgctattcca cccaggatta cctgggcctg catgatgcgt
    68461 tagtcctcat tgacgacgcc aagcatttgt ctgccgatga agacaaaaac accatgcaga
    68521 cgtactcacg tatgaaaaac cagtttaccg tcagcattaa ccagccattg aatatcgcct
    68581 atgaagatta cggttcgctg tttatttccg gtagctggac gtattactgg gcggcgaaca
    68641 atagccgcac tgaatataat gttggttaca gtaaaagcgt ttcgtggggc agtttcagcg
    68701 tcaacctaca acgtagctgg aatgaagacg gcgagaaaga tgacgcgatg tacgtcagcg
    68761 ttagcgtacc tattgagaat attttaggtg gcaaacgtaa gtcttctggt ttccgcaatt
    68821 taaatactca gctcaatacc gatttcgatg gttcacatca gttgaatgtt aacagttccg
    68881 gtaacactga aaacaatctg gtgaactaca gtgtcaacgc aggttatagc ctcgataaaa
    68941 acgccggcga tttagcctct gttggtggtt atctcaacta tgaatctggg ttaggcggta
    69001 tttccgcttc ggcctcggcc acttctgata acagccaaca gtactccatc tcaaccgatg
    69061 gcggctttgt attacacagt ggtggtttaa cgttcactaa caacagtttc agcagtaacg
    69121 acacgctggt gttaatcaac gccctaggtg ctaaaggcgc acgaatcaat aacagtaata
    69181 acgaaatcga tcgctgggga tatgccgtga cgtcctctgt cagcccatat cgtgaaaacc
    69241 gggtaggtct gaacattgaa acactggaaa acgatgttga actgaaaagt accagcgcca
    69301 ccaccgtacc acgtagcggc tccgttgttt tgacccgttt cgaaactgac gaggggcgtt
    69361 ctgccgtgct gaatattact gccgccaatg gcaaatccat tccgtttgct gcggaggttt
    69421 accagggtga ggtgatgatc ggcagcatgg gccagggtgg tcaggcattt gtacgcggta
    69481 ttaacgacag cggggaatta atcgtgcgct ggtatgaaaa caaccaaacc attgactgta
    69541 agttgcacta ccagttcccg gcgcagccac aaacgcaggg aagcaccaac accttattac
    69601 ttaacaatct tacctgtcag gtagcaaatc actaatatgg aaaattctat gaaaaggata
    69661 ctactaacat ccgcgttaat aggcctgggt ttaccggcgg ttggctctgc aacagatctt
    69721 aatgtcgatt ttacggccac cgtattagcc accacctgca cgataactat agtggaagat
    69781 ggtggtccag cagtgaccgg tagtaacgat gaatacagcc tgacaattcc tgatgttggt
    69841 ctggataaag tcgctaccgc tgcgcccgaa gctcaggcca actttaaatt aaaggcctcc
    69901 gattgcagca atggttactc aaagattttt accacactta caggaacaac ggtttccggt
    69961 aagttaattg ttaatgaagc aacctctggc gctgctgggg tggggatggg gattaagcgt
    70021 agagataccg cagactcgac tttcttcaca cccaataata ctgacaaatt tgaatggagc
    70081 gccgatgaga aagccagcgg tgtgcctctg accgtagcac tgcgcgaaac aacggcggga
    70141 gcaggacgca ccggtgcgtt tcaggcaaaa gccaccttta acttcaccta cgaataacag
    70201 ggacagccac catgaaatta aaagtcatcg ctacactgat tgctactgtt gccgtgggtg
    70261 taagctttaa cagcaatttt gcttctgcga gtacaacgtc cgcttcttta accgtaaaca
    70321 gtaacctgac tatgggtacc tgcagtgctc agataatgga taatagtaat aaagtgatca
    70381 atgaagtggt ctttggcaat gtttatattt ctgaactcgg tgcaaaaagc aaagtgcaac
    70441 agtttaaaat tcgctttagc aattgctctg gccttcccca aaacagcgcc caaatagtgc
    70501 tggcacctaa tggtatatcc tgtgctggtt ctcaatcgtc atcggcgggt ttttctaaca
    70561 agtttactga cgctagcgca gcaaccagaa cggctgtgga agtatggact acagatacac
    70621 cggaaagcaa tggcagtacg caattccatt gtgctcaaaa gataccagtg cctgtgacgc
    70681 ttcccgccga caccacaact cagccttacg attacccgtt aagtgcacgg atgaccgttg
    70741 cggaaggtag attggtaacc gatgtaagac cgggtaattt ccgctctccc acgactttca
    70801 cgatcactta tcagtaatag cggataagga tattagaatg ctatgcaggc atcataaaat
    70861 cgtacacttt ttagggctgg caacagccct tatcacgcct tttgcctatt ccggccagga
    70921 tgtggatctc accgcaaaga tcgtgcccag cacctgtcag gttgaagtca gtaataatgg
    70981 cgttgtcgat ctcggcacag tgacgctgga ctattttgcc gacaatgtaa ccccgacaac
    71041 ggattatgcg ggtggcaaaa cgtttaatgt gaatgtggtc tcttgcgata acattcaaac
    71101 gacacagtcg caaatgaaat tagattttca gccgcaggcg ggatcgcttg cacaggcgaa
    71161 taatcaaatt tttagtaatg aatatgaaca gcaggctacc ggggcgaaaa acgtcggcat
    71221 tgtgattttt tcggcccaac cgaatcagca gacatttaac gtgcgaggaa cggatggttc
    71281 ctcaacagca atttattcgg tagcacctgg caatgctgtt ccctcaacct ggacctttta
    71341 ttcccgtatg cagcgggtga ataatgccct tcctcctgaa tcgggaatgg tgagaagcca
    71401 ggttattgtt aacgtatctt acgaataata aggataatta tgcaaaggaa aggcaataaa
    71461 ctgttgattc agttatgcag tgtgatactg ctatttttta ccacatcctg gtatgcattg
    71521 gcgaatgaat gttatataga gagaaatgct gaaggggatt atcacatgaa gataagctct
    71581 actcagctta gtctggcgtc acaaatggtc gaggttccga cagaaatagc cgaagctaca
    71641 tgggatgtaa atattcaact aagaggcgat gccatagggt gtaaatctct tggggatagt
    71701 aaggcagttc actttcttaa tacagctgac ccaagtttaa tatccacgta caccacaacg
    71761 aatggcgcag cgttattaaa aacaactgtt ccaggcattg tgtattctgt cgagttatta
    71821 tgccttagtt gtggtgccgc agatgaactt gatttatggc tacctgcaca aagtggcgca
    71881 gataacttca taccaagcac ccagacgaaa tgggcctatg agtacagtga tcaaagttgg
    71941 tatttacgtt ttcgcttatt cataactcct gaatttaaac ccaagaatgg tgtttccagc
    72001 ggaacaacga tagcaggaaa gattgcgtca tggtatatag gtaccaatga ccagccgtgg
    72061 atcaactttt acattgacaa tgactcttta aagtttttcg tcgatgaacc gacctgtgca
    72121 acagttgccc tggcacaaga tcagggcaac gtcagtggca atcaggtaac gcttgggaac
    72181 agctatgttt cggaagtgaa aaatgggctt acgcgggaaa tccctttttc tatccgtgct
    72241 gaatactgtt atgccagtaa aattacggtt aagttgaaag cggcaaataa acccagcgat
    72301 gccacactgg tgggtaaaac gactggctcg gcttcaggcg tggctgtaaa agtaaattca
    72361 acttatgaca atagcaaagt attgttaaaa gcagatggta gcaacacggt tgactacaac
    72421 ttcgccgcct ggtcaaacaa cctgctgttt ttacctttta cggcgcagct ggtaccggat
    72481 ggtagcggta atgctgtcgg tgttggaaca ttttcaggta acgcgacctt ctcctttacc
    72541 tacgaataag taaaacgtca ttccctcctc atcgcaccag agttctgctc tggtgcgaac
    72601 atttccccac actaataacc attgcctccc tgttttcggc tgttagaatg cgatcaatct
    72661 gactttagcc atcaggatac gttatgaaac cgaccaccat tgcttcattg cagaaatgta
    72721 agcaggaaaa aaaacgcttc gccaccatca ccgcctacga ctacagcttc gcaaaactgt
    72781 ttgccgagga agggctgaac gtgatgctgg tgggcgactc gctggggatg accgttcagg
    72841 ggcatgaatc tacccttccc gttaccgttg aagatatcgc ctaccacact accgcagtac
    72901 gtcgtggcgc gccaaactgc ctgctgttgg ctgacctgcc gtttatggcg tatgccacgc
    72961 cggaacaagc ctttgaaaac gccgcaacgg ttatgcgtgc cggtgctaac atggtcaaaa
    73021 ttgaaggcgg tgagtggctg gtcgaaaccg taaaaatgct gaccgaacgt gccgttcctg
    73081 tatgtggtca cttaggttta accccacagt cagtgaatat tttcggtggc tacaaagttc
    73141 aggggcgcgg cgatgaagcg agcgatcgac tgcttagcga tgcattagcc ttagaagccg
    73201 ctggtgcaca gttgctggtg ctggaatgcg tgccggttga actggcaaaa cgtattaccg
    73261 aagcgctggc gatcccggtt atcggtattg gcgcaggcaa cgttaccgat gggcagatcc
    73321 tcgtgatgca cgatgcgttc ggcattaccg gcggtcatat ccctaaattt gccaaaaatt
    73381 tcctcgctga aacgggcgac atccgcgctg cggtgcggca gtatatggct gaagtggagt
    73441 ccggcgttta tccgggcgaa gaacacagtt tccattaagg agtgacgttg tgttaattat
    73501 cgaaaccctg ccactgctgc gccagcaaat tcgccgcctg cgtatggaag gcaagcgcgt
    73561 ggcgctggta cctaccatgg gcaacctgca tgatggccac atgaagctgg tcgacgaagc
    73621 caaagcccgc gccgatgtgg tcgtcgtcag tattttcgtt aacccgatgc agttcgaccg
    73681 cccggaggac ctggctcggt acccacgcac cttgcaggaa gactgcgaaa agctgaacaa
    73741 gcgcaaagtg gatctggttt tcgcgccctc ggtaaaagag atctacccga acggtactga
    73801 aacccatact tacgttgacg ttccagggct ttcgaccatg ctggaaggcg ccagccggcc
    73861 ggggcatttt cgcggcgtgt cgactatcgt cagcaagctg tttaacctgg tccagccgga
    73921 catcgcctgc ttcggtgaaa aggactttca gcaactggcg ctgatccgca aaatggttgc
    73981 tgatatgggc tttgatattg agattgtcgg tgtgccaatt atgcgcgcca aagacggtct
    74041 ggcactgagt tcccgtaacg gttatctgac ggcagaacaa cgcaaaattg cgcccggtct
    74101 gtacaaagtt ttaagttcga ttgccgacaa attgcaggct ggcgaacggg atctcgatga
    74161 aattattgct attgccgggc aagaactgaa tgaaaaaggc ttccgcgccg atgatattca
    74221 gattcgcgat gccgacacat tgctggaagt ctctgaaacc agcaaacggg cagtaattct
    74281 ggtagccgcc tggcttggcg atgctcgcct gatcgacaac aaaatggtcg agctggcgta
    74341 atacttatac tctaaataat tcaagttgca agttgcagga aggcggcaag cgagtgacaa
    74401 attcgtcagg aacgaatttg cccagccgca ggctggcctt cggtgagaga catggatgtc
    74461 tctcattaat ccccaggagc ttacataagt aagtgactgg ggttagcgaa cgcagacgca
    74521 gcacatgcaa tttgaagtat gacgagtata actggcgcta cggctgatgg cgccaattat
    74581 taatttgccc cacgtcatgg cgatggcgtc tgtaagcaat tattcataac gcagacaact
    74641 tagttaatca gcctatcaat ctcagcaaga gataaatttg tcatctctgc aacgtcttcc
    74701 cgagacatcc ctttactcag aaaacgctgg gcgaattctt gcctctcctc ctgtcttccc
    74761 tgctgaatcc ccttctcaat ccctttctct tcaaaccact gtgccagcgt catcatagac
    74821 ttccctcccg tttccctgtc tctcagcaca ccataaaaca aatccgcttg ttcagtatga
    74881 ccgcgttgca gcatatagtt ttgcatggca actaactgac tttcgctagt gtacccttcg
    74941 tcaatcagcg tgaccagttg ctccagcaat aacattaagt cgcgctggcg aatatgtttt
    75001 tgcagtagtt cgagaatcgc aatccgccga tgttgcatga tttcgtcatc cggtgtgatg
    75061 gtgatatcca ccagcgggaa aggactgtta tagacgcgtc gcgccagctc cggcgagtaa
    75121 aacatatcaa accagcacat tgagagcgga taaggtgtgg cctcgccctg ataaaagaga
    75181 atcggcacta ccagcggtag tttgtcgtga tccgcctcca gatgccgatg catggcggct
    75241 atagaataac gcatcatgcg aaaggccatt ttcttatccg gcttgctttg gtgttcaatc
    75301 acaacatgca gataaccggg attgccctgc atttgcacgg aatagagcac gtccgtgctg
    75361 tgtcctttaa ggctctcttc aatgaaactc cccgactcta aatgaagcgt gttgaggtca
    75421 caaagttcgc gtaattccac tggcaaatgt atctccagaa agtcgcgagc cgtctccgca
    75481 tgcattaaaa attgtttaaa taccgcgtca tgcggtgtgg tactcggtgc atccatcttc
    75541 cctgtcttcc gtgttgtctt gcgtgaaggc agtatccagt caaggcgtgc tatcgtcagc
    75601 cacctcttta ttgtttaccg agcagcgttc agagaaatat caattcgtta caggcgatac
    75661 atggcacgct tcggcgcggt acgcaaactg ctgatgcaaa tgccgtcaga tgagtagaca
    75721 ctaaacaaaa atcgggcaat actgcgtgag aatttcctca aagcaggcac cgcctgcttc
    75781 gttaacgaca gggtagaaag gtagaagtta tgattcgcac gatgctgcag ggcaaactcc
    75841 accgcgtgaa agtgactcat gcggacctgc actatgaagg ttcttgcgcc attgaccagg
    75901 attttcttga cgcagccggt attctcgaaa acgaagccat tgatatctgg aatgtcacca
    75961 acggcaagcg tttctccact tatgccatcg cggcagaacg cggttcgaga attatttctg
    76021 ttaacggtgc ggcggcccac tgcgccagtg tcggcgatat tgtcatcatc gccagcttcg
    76081 ttaccatgcc agatgaagaa gctcgcacct ggcgacccaa cgtcgcctat tttgaaggcg
    76141 acaatgaaat gaaacgtacc gcgaaagcga ttccggtaca ggttgcttga ttgttatccc
    76201 tgcggctggt tactcaccag ccgcgacatc gtctccagcg aatccgttct taagatataa
    76261 agtcgtttta gtaacaacgg attatccccc ggttttactt tgcctttcat ggttgtcacc
    76321 gccaggtgaa atccggcatc gtttgctgcc ttcacggctt tgtcattaaa gccgccaaac
    76381 ggatacgaaa gataccagac atgcggatta aattgcgcca gagcgcgacg tgaacgtgca
    76441 aaatcaaaca gaatattgtg ctcactacgg ctcagtaata tgggtcggcg ataaccatct
    76501 acccgatgca aaaaatgggt atgtgactgg aaatcaaata catcgcgaat ttcgttaagc
    76561 tcagaaacgc tcataaattg cagcgatttt ggttcccact tctgcgggtg acgtttgatg
    76621 cgcgaggtaa caataaacgc cgtcgccttc atgccatatt gtttcaacac aggatacgca
    76681 tagcggctca ctgacttgag gccatcatca aaggtaatca ccaccgctcg cgcaggaaga
    76741 ttgatcttat tcttcacgta accttccagc tgcaccatgc tcagtgtcgc gtatcccctg
    76801 tcgcgcagcc aggccatctg gttattgaaa gcgcgtaccg aggtggtcgt cgaagtatgg
    76861 cgaaaacggg tgttttcttc gtcgcgcaga atatggtgat aggttagcac cggaaggcca
    76921 ttatcgggtt gggcatccag cgcgctgata taggccagtc gatcgccaat acggatctga
    76981 taccaggttt gatttaacct gtctttcagt ttatgcaaaa tcgggtagcg caaattgtcc
    77041 gccagtaccc caaatggcgc actgcccaca ctcggcgcgt tatagaccgg cgtatctttc
    77101 caggtaacta agttctgatt actcagcggc ttgttgaggt cacccaaacc gtcttcaact
    77161 ttttgtcgcc cctgaaccgg ctcaagatga cctttatcga taaacccttt atcaaagcca
    77221 aaattaaatg cgtaataact tgcggcagtg ggctccacgg caatgatttg tccggcccga
    77281 atattcccca cggtcaccat cttgtcacca atttgcgccc agaccgcagc attttcgatg
    77341 gtttgcatat aacgggcagg taacgcggca ctgacacttg cggtaaacag catcagcagg
    77401 agaataacag cttgtttgta catgaaaaaa tcaccgaggg tgaaacaacg cttttattgt
    77461 agcaaaagcg ccatcagaat cgcgggttaa tactcataca aattatgttt gaggacgaaa
    77521 ggcggatttt tttgttgctg gtgccaaata ctactcacct ccggcgcacc cagttcgaca
    77581 atacgctcgc gaaactctga actggtcatg gtttcacggc aagccatcat ctgttcaatt
    77641 aacggtaacg tgacaccaga aatcacttcg caacgggagt gtttgtggct taataatgaa
    77701 gccacgcggt acggtggcgc tccggctata tcggttaaga agatgacacc ctcaccagag
    77761 tccgcttcat gcagagcatc gcacatcatg cggctgagca tattagaact taatccgcgc
    77821 cagaaattca cggcccggca ctgaagaagc gccccatgtt ttttctccag cacatccagt
    77881 atctcttgcg cccggtcatc gtgacaggta attacccaac ctaacatcgc cttcctcctt
    77941 agccgaaaat cagtctaccc tgaccattgt tgataaaggg tgatagccgt caaaactcct
    78001 ctcccccaaa tccagaggag aggaaatagc cttagctacg caaaccacgt ccacgttgga
    78061 tcagcgacca acagatcaaa taaaacgcca caataaagac caccagtacg ccaaaggtag
    78121 tgaccagcgg aacatcattg ataccgagga agccgtagcg gaaaccactg atcatataaa
    78181 cgattgggtt caggtgcgac agcccttgcc agaacggcgg caacaaagtc agtgggtaaa
    78241 agaccccacc caaatacgtg agtggcgtta acacaaaggt tggcaccagg ctgatgtcat
    78301 cgaacgtttt ggcaaacaca ccgttcagca aacccgcaag ggagaacaac accgccgtga
    78361 gcaccagggt taaggcaacg aatacccacg aatgcacctg aaacggcaca aaaaacagtg
    78421 aaattgccgt caccagaatg ccaacaaaca gaccacgcgc cacgccaccg ccgacatatc
    78481 cggcaataat gacgtgagtc ggaaccggcg ctaccagcag ctcttcaata ttacgctgga
    78541 acttggcacc aaaaaatgat gacgcgacgt tggcgtaggc gttggtgatc accgacatca
    78601 tgatcagccc cggtacgatg aactgcatgt agctgaagcc atgcatatcg ccaatacgcg
    78661 aaccaatcag gttaccgaaa ataataaagt aaagggtcat ggtgatgact ggcggcacca
    78721 gcgtctgcac ccagatacgc ataaagcgat ggatctcttt cgcccagatg ctttttagcg
    78781 ccacccagta aagatgcatc atgcgcgatc tccttgtttt tcattaacca gtgaaacaaa
    78841 cagctcttcc agacggttag ctttgttacg catacttaat acctgaatgc cctgctcact
    78901 taactgcgta aatacgctgt tgatcccctg ctcacgcagc acttcaactt ccagcgtcgc
    78961 ggtatcgacc agtcgatact gatagccatc gagcttcggt aacggacttt tcggtgcgag
    79021 atcgagaata aaggtttccg atttcagctt cgccagcagc gccttcatcg aggtattttc
    79081 caccagctca ccgtgttgaa taatgccgat attgcggcac agcatttctg cttcttccag
    79141 gtagtgtgtg gtgagaatga tggtggtgcc tttgtcgttt aaatccttca aaaagcccca
    79201 cattgagcgg cgaagttcaa tatccacgcc tgcagtcggc tcgtcgagaa tcagtagttt
    79261 aggttcatgc attaacgcac gggcaatcat taaacggcgc ttcatcccgc cagataacat
    79321 gcgcgcacgt tcgttgcgtt ttccccataa atcgagctgc ttaagatatt tttcgctgcg
    79381 gatgtacgcc tctttgcgct ccacaccgta gtaccctgcc tgattcacca caatttgctg
    79441 cacggtttca aacgggttga agttaaattc ctgcggcacc agtcccaact gacgtttagc
    79501 gttcacgaca tctttctcga gatcgtaacc aaatacgctg acccgcccgg aggttttatt
    79561 taccagagag ctgataatac cgatagtggt cgatttcccg gccccgttcg gcccgagaag
    79621 cgcataaaaa tcacccgctt cgacctgcaa atctattcca cgaagcgcct gaacgccgcc
    79681 tggatagttt tttttaagct gttgaagttc cagtgcaatg gtcataaatt tttacttacc
    79741 ttacgttctt acactttatc tgtggtttaa atcgtcccgg agttgcccta tattagccaa
    79801 acgtaattat ttggttacag gtcgttaacc tccatgaaag acatagatac actcatcagc
    79861 aacaatgcac tatggtcaaa aatgctggtg gaagaggatc ccgggttttt tgagaaactg
    79921 gcacaagcgc aaaaaccgcg ctttctatgg attggatgtt ccgacagtcg cgttcctgca
    79981 gaacgtttaa ccggtcttga gccgggcgaa ctctttgttc accgtaatgt tgctaacctg
    80041 gtcattcaca ccgacctgaa ctgcctttcc gtggttcagt atgcagtgga tgtactcgaa
    80101 gttgaacaca ttattatctg tggccactac ggttgcggcg gcgtacaagc cgcagttgaa
    80161 aacccggaac tggggcttat caacaactgg ctgctgcata tccgcgatat ctggttcaaa
    80221 catagctcat tgctcgacga aatgccgcaa gagcgccgtc tggatacctt gtgtgaactg
    80281 aacgtcatgg aacaggtgta taacctgggc cactccacca ttatgcaatc agcgtggaaa
    80341 cgcgggcaga aagttaccat tcacggctgg gcttatggca ttcacgacgg cttgctgcgt
    80401 gatctggatg ttactgccac caaccgcgaa acccttgagc aacgttaccg tcacgggatt
    80461 tccaacctca agctgaaaca cgccaaccac aaataaaaat gccatgccgg atgcaacaca
    80521 tccggcaact tcacacttac tcgtccagca gaatcacttt gccgatatac ggcagatgac
    80581 ggtaacgctg tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg atcgagaaac
    80641 cgataaattc taccgggacg ttcacttcac gacgggacgg tttatccagc agcgtacaaa
    80701 tcgccaacga cttcggttcg cgcaggctta agatctcacg cactttcgac agtgtattcc
    80761 ccgagtcgat gatatcttca acaatcagca catccttgcc acggatatct tcatccagat
    80821 ctttgaggat tttcacatca cgggtggtgg acatgccgct accgtagctg gaggcggtca
    80881 taaagtcgac ttcatgagat acctgaactt cacggcacag gtccgccata aacataaatg
    80941 agccacgcag cagacccacc agcaccatat cgctgccgct gtctttgtaa cgctcagtaa
    81001 tctgacgacc cagttcggcg atacgcgctt taatctccgc ttcggggatc attacttcta
    81061 cagtatgttt catatctcta accatatgat ttaaaaacaa atcacttcgc acgggcaggt
    81121 tttggcaccg caacaaagcg caagacacgc agtataccag caaaacgatt cattcgcgga
    81181 atctgttaat aaagctcaaa ttgtgatgac gatcacacat gttaaaacct ataattaatt
    81241 gctactaaaa tattaatgaa ttgaaatggt gtctctttat ggcaattaac aatacaggct
    81301 cgcgacgatt actcgtcacg ctaacagccc tttttgcagc gctttgcggg ctgtatctac
    81361 tcattggcgg aggctggctg gtcgcgattg gcggctcctg gtactaccct atcgctggcc
    81421 ttgtgatgct cggcgtcgcc tggatgctgt ggcgcagtaa acgtgccgcg ctttggctat
    81481 acgccgccct gctgctcggc accatgattt ggggcgtctg ggaagttggt ttcgacttct
    81541 gggcgctgac tccgcgcagc gatattctgg tcttcttcgg catctggctg atcctgccgt
    81601 ttgtctggcg tcgcctggtc attcctgcca gcggcgcagt tgccgcactg gtggtggcac
    81661 tgctgattag cggtagtatc ctgacctggg ccggatttaa cgatccgcag gaaatcaacg
    81721 gcaccttaag cgccgatgcc acacctgcgg aagctatctc acccgttgcc gatcaggact
    81781 ggcctgccta tggtcgcaat caggaaggtc aacgcttttc acctctgaaa caaattaacg
    81841 ccgataacgt ccataatctg aaagaagcct gggtgttccg tactggcgat gtgaagcagc
    81901 cgaacgatcc gggcgaaatc accaatgaag tgacgccgat taaagtgggc gacacccttt
    81961 acctgtgtac cgctcaccag cgcctgtttg cgctcgatgc cgccagcggc aaagagaaat
    82021 ggcattacga tcctgagctg aaaactaacg agtctttcca gcacgtaacc tgccgtggcg
    82081 tctcttatca tgaagccaaa gcagaaacgg cttcgccgga agtgatggcg gattgcccgc
    82141 gtcgtatcat tcttccggtc aatgatggtc gcctgattgc gattaacgct gaaaacggca
    82201 agctgtgcga aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat atgccagaca
    82261 ccaaaccggg tctgtatgaa ccgacttcgc caccgattat caccgataaa accatcgtga
    82321 tggcaggttc agttaccgat aacttctcaa cccgcgaaac gtctggcgtg atccgtggtt
    82381 ttgatgtcaa caccggggag ctgctgtggg cttttgatcc gggcgcgaaa gatccgaacg
    82441 caatcccgtc tgacgaacac acctttacct ttaactcgcc aaactcgtgg gcaccagcgg
    82501 cctatgacgc gaagctggat ctggtgtatc tgccgatggg cgtgaccacg ccggatatct
    82561 ggggcggtaa ccgcacaccg gaacaggaac gttatgccag ctcgattctg gcgctgaatg
    82621 ccacaaccgg gaaattggcg tggagctacc agaccgttca ccacgacctg tgggatatgg
    82681 atcttccggc acagccgacg ctggcggaca tcaccgttaa tggtcagaaa gtgccagtta
    82741 tttacgctcc ggcgaaaacc ggcaacattt ttgtgctcga tcgtcgtaat ggcgaactgg
    82801 tggttccggc accggaaaaa ccggttcccc aaggtgctgc caaaggcgat tacgttaccc
    82861 ctactcaacc attctcggaa ctgagcttcc gtccgacgaa agatttgagc ggtgcggata
    82921 tgtggggagc caccatgttt gaccaactgg tgtgccgcgt gatgttccac cagatgcgct
    82981 atgaaggcat tttcacgccg ccatctgaac agggcacgct ggtcttcccg ggtaacctgg
    83041 gaatgtttga atggggcggg atttccgttg atccaaatcg tgaagtggca attgccaacc
    83101 caatggcact gccgtttgtt tcgaaactga tcccacgcgg tccgggtaac ccgatggagc
    83161 agccgaaaga tgccaaaggc acgggtacgg aatccggcat tcagccacag tacggtgtac
    83221 cgtatggtgt cacgctcaac ccgttcctct caccatttgg tctgccatgt aaacagccag
    83281 catggggtta tatctcggcg ctggatctga aaactaatga agtggtgtgg aagaaacgta
    83341 ttggtacgcc gcaggacagt atgccgttcc cgatgcctgt accggtgccg ttcaatatgg
    83401 gtatgccgat gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt atcgccgcta
    83461 cggcagataa ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg tggcagggtc
    83521 gtttaccagc gggtggtcag gcgacgccaa tgacctatga agtgaatggt aagcagtatg
    83581 tggtgatctc cgcaggcggt cacggttcat ttggtacgaa gatgggtgac tatattgtgg
    83641 cttatgcgct gccggatgat gtgaagtaag acttgctcag attgctgaca acgtgcgcgt
    83701 tgttcatgcc ggatgcggcg tgaacgcctt atccagccta caaaattgtg caaaatcaat
    83761 ggattgcaca ggtaacgtag gcctgataag cgtagcgcat caggcagatt tgcgtttgtc
    83821 atcagtttaa tgcccggaga gatccgggca tattcccgaa tacggtcttt ttataccgta
    83881 aaccctaaca tcatccccgt atcttcatgc tccagcagat ggcagtgcgc catataagca
    83941 cgttctttcg gtgcgtcgtg attaaacttc accagcactt cactgacatt gccttctacc
    84001 ttaacggtat ctttccagcc cgcgcgatga gccgctggcg gtttgccatt ttctgacaag
    84061 atacggaact gcgtgccgtg aatatggaac ggatgcagca tcatgtcgcc cacgccagag
    84121 ataacccaac gttcgtactg ccctttcgcc gccgcaaaca tcggcttatt catatcaaac
    84181 gcctgaccgt tgattttatt ggcatggtgg aaatcgaact tcccgccgtg gttcatatga
    84241 ttcatattgc cgtgccccat atggcccatc atctggctgt gatccattcc ggccatcgcc
    84301 tgatcgccat atttctccat tagcatctgc atccccatca tatcgagcat cgggtccata
    84361 gaaagttgca gcttgcgtac cgtcagccct tccagcgaag gtaacgcagg caggctactt
    84421 aatgtgtctg gcaaagcacc ggaagcacta atagcaatcg gctgaatccg cattaccgga
    84481 tgaggcttat caaacggcgc aatcgccatc cccatctggc tgaccggcag cgtcaccagg
    84541 tcaaagggtt tgttgtcgtt aacctccacc agcacttcaa aacgctcgcc catcagcacc
    84601 ggcagctcgt tcaccttcac cggttcaggt agcagaccac cgtcgctggc aatcacataa
    84661 agcgggcgat tgtcgctggt ggcgaaattg agcgagcggg cattacagcc attgagcaaa
    84721 cgcaggcgca gccaaccacg cggggcagcg tgttgcgggt agattgcacc gttggtcagc
    84781 aacgtatcgc caaaccagcc cacggcggcg gtcatcacat ccagttgata atcaatctgc
    84841 ccgtcggcgc taaatttctt atcctgaacg atcaccggaa catcatcgat accccactgt
    84901 tttggcagca ttaatttcag gatctcgtca tcttcaatca ccaccagacc agccagcccc
    84961 atcgccacct gtcgcccggt cttgccatgt tgatgtggat ggaaccagca ggtagcggca
    85021 ggttgatcaa cgttcaacgt caccgagcgc ttgccacctg gcggaataat tccctgtggg
    85081 ccgccgtcga cttcacccgg tacttccagc ccgtgccagt gcaacgtcgt ctcttccgtc
    85141 agttggttat agatatcaac cgttaccgct ttgccgcgct gtaatttcac cgccggcccc
    85201 agcagattgc cgttatagcc ccaggtagtt gcagttttct cgccaaaggt agactgacct
    85261 gcgccaatag ttaactgaat gcgattacgg gcatcggtcg tgagcaaatc agggatcggt
    85321 aacgttgggc gttccgccgc aaagactgca cggctccaca gtggcaaggc tgaagccaca
    85381 cccagtgcga cggaatattt caagaaatca cgacgttgca tagttatttc cttattctta
    85441 agcaggcgaa acaaaatcaa acgttgagca tagtccttcc ccttacggga aggtcaagcc
    85501 gcagacacaa tttaatcgac aataatggtc gtcgcctcgc tcgcagtgtg ctaacgttta
    85561 tcttctttaa gcccctggta gaagcaatga agacgttttt cagaacagtg ttattcggca
    85621 gcctgatggc cgtttgcgca aacagttacg cgctcagtga gtctgaagcc gaagatatgg
    85681 ccgatttaac ggcagttttt gtctttctga agaacgattg tggttaccag aacttaccta
    85741 acgggcaaat tcgtcgcgca ctggtctttt tcgcccagca aaaccagtgg gatctcagta
    85801 attacgacac cttcgacatg aaagctctcg gtgaagacag ctaccgcgat ctcagcggca
    85861 ttggcattcc cgtcgctaaa aagtgcaagg ccctggctcg cgattcctta agcctgcttg
    85921 cctacgtcaa ataatccctg ataccttttt gtagaaatat tgaccgtgca tccgcggtca
    85981 atgttagcta ttatgttgcg cccttttttt acgggtgtta acaaaggagg tatcaaccca
    86041 tggccgaaaa aaaacagtgg catgaaacgc tacacgacca gtttgggcag tactttgcgg
    86101 tagataacgt tctgtatcat gaaaagaccg atcaccagga tctgatcatt tttgagaacg
    86161 ctgcatttgg tcgcgtaatg gcgctggatg gcgtagtaca aaccaccgag cgcgacgagt
    86221 ttatctatca tgagatgatg acccatgttc cgctactggc ccacggtcac gcgaaacatg
    86281 tgctgattat cggcggcggc gacggtgcca tgctgcgtga agtaacccga cataaaaacg
    86341 ttgagtcaat cacgatggtg gaaatcgatg cgggtgtcgt gtcgttctgc cgtcagtatc
    86401 tacccaacca taacgccggt agctacgacg atccgcgctt taagctggtg atcgacgatg
    86461 gcgtcaattt cgttaatcaa accagccaga cctttgatgt cattatctcc gactgcaccg
    86521 atcctatcgg tcccggcgaa agccttttca cttcggcatt ttatgaaggc tgcaaacgtt
    86581 gcctgaatcc tggcggtatc ttcgtcgcac aaaacggcgt ctgcttttta cagcaggaag
    86641 aagccatcga cagccatcgc aaactcagcc attacttcag cgacgttggc ttttatcagg
    86701 cggcgatccc gacctattac ggcggtatca tgacttttgc atgggcgaca gataacgacg
    86761 ccttacgcca tctctcaacc gaaattattc aggcgcgttt tctcgcctct ggcctgaaat
    86821 gccgttatta caatccggca gtccatacgg cagcttttgc cttaccccag tatctgcaag
    86881 acgcactggc ttcacagccg tcctaaggag aagataagaa attgaaaaaa ctgaaactgc
    86941 atggctttaa taatctgacc aaaagtctga gtttttgtat ttacgatatc tgctacgcca
    87001 aaactgccga agagcgcgac ggttatattg cttatatcga tgaactctat aatgccaacc
    87061 gtctgaccga aatcctgtca gaaacctgtt ccattatcgg ggctaatatt cttaacatcg
    87121 cccgccagga ttacgaacca cagggtgcca gcgtcactat tctggtgagt gaagaaccgg
    87181 ttgacccgaa actcatcgac aaaacagaac accccggccc actgccagaa acggtcgttg
    87241 cccatctcga taaaagtcat atttgcgtac atacctaccc ggaaagtcat cctgaaggcg
    87301 gtttatgtac cttccgcgcc gatattgaag tctctacctg cggcgtgatt tctccgctga
    87361 aggcgctgaa ttacctgatc caccagcttg agtccgatat cgtaaccatt gattatcgcg
    87421 tgcgcggttt tacccgcgac attaacggta tgaagcactt tatcgaccat gagattaatt
    87481 cgattcagaa ctttatgtct gacgatatga aggcgctgta tgacatggtg gatgtaaacg
    87541 tctatcagga aaatatcttc cataccaaga tgttgcttaa agagttcgac cttaagcact
    87601 acatgttcca caccaaaccg gaagatttaa ccgacagcga gcgccaggaa attaccgctg
    87661 cgctgtggaa agaaatgcgc gagatttatt acgggcgcaa tatgccagct gtttaacggc
    87721 tctggcggag ctcccaggct ccgccagatt tatttacttc tgctgcacga aattgcggta
    87781 agccgccacg acctgcagaa aatcctcaac gccgcatagc gacaggcttt cttcgtcgta
    87841 gtagttcatc ccctcttcca tttcatcgcc agcgaattcc agttgattgg cgcgaaccat
    87901 cacctcttcg ccgtccatcc atagcgtata ctcatgccct gcccgctgcc aggaacgttc
    87961 gctacctttc agtgcgtgcg ctgcttgttc cacttcatca agcaaggcca ggttttcttt
    88021 cacctcttca ttaaaccagt gcccgaccac ttcatgcccc atggacatac gcacctttac
    88081 cactccggta atatcgcgca gaaattcgta atccatagtg tgttcctctg ctcccggcaa
    88141 tgcaccgaaa tcgcattgcg ccattgatgt aattatcgca gccttaccgg agaaaaacag
    88201 cggaacggcg atggtttaga aataaaaaaa tgcccgggaa tctcattcac cgggcattgt
    88261 gtcgtttatg cgcagcgcgt gcgctgactt tttaaaccgc agtctggaaa atcaccccat
    88321 cggctttctc ggtgtactga gaaagctggt tgaagttcag ataacggtaa gtatcaacgg
    88381 ctgttttatc tacctgcgcc acgtaggtct ggtactcttc cggcgtcggc agtttgccaa
    88441 tcagcgccgc aacagcagcc agttccgcag aagccaggaa gacattcgcg ccagtaccca
    88501 gacggttcgg gaagttacgg gtagaggtgg aaaccaccgt tgcaccgtcc gccacacgcg
    88561 cctggttacc catacacagg gaacagccag ggatctcgat acgcgcacca ctcttaccga
    88621 agacgctgta gtagccttct tcggtcaact gtgcggcgtc catacgggtt ggcggtgcca
    88681 cccacaggcg ggtcggcaac tgacctttgt gcgcatccag cagtttacct gcggcacgga
    88741 agtgaccgat gttagtcatg caagaaccga taaacacttc gtcgatcttc tcgccctgta
    88801 ccgcagacag cggacgtgcg tcgtccgggt cgttcggagc acagaggatt ggctctttaa
    88861 tatccgccag atcgatgtcg atcactgccg cgtattccgc atctgcatcg gcttccagca
    88921 gctcaggatt cgccagccat ttttccatgc cctgaatacg acgttccagg gtacgacgat
    88981 cgccgtaacc ttccgcgatc atccacttca gcaggacgat gttggagttc aggtattcga
    89041 tgattggttc tttgttcagc ttgatggtac aaccggcagc agaacgctcg gcggacgcat
    89101 cggtcagctc aaaggcctgc tcaactttca gatccggcag accttcaatt tctaggatgc
    89161 ggccagagaa gatgtttttc ttgcctttct tctcaacggt cagcagacct tgtttgatcg
    89221 catacagcgg aatggcgtgt accagatcgc gcagggtgat gcccggctgc attttgcctt
    89281 tgaagcgcac cagaacggat tccggcatat ccagcggcat tacgcctgtt gcggctgcaa
    89341 atgccaccag accagaaccc gccgggaaag agataccgat cgggaaacgg gtatgggagt
    89401 caccaccggt accgacggta tccggcagca gcatacggtt cagccaggag tggattacgc
    89461 cgtcacccgg acgcagcgac acaccgccac ggttcataat gaagtccggc agcgtgtggt
    89521 gcgtgttcac gtcaactggc ttcggatacg ccgcggtgtg gcagaaagac tgcatcacca
    89581 ggtcagccga gaagcccagg cacgccaggt ctttcagttc atcacgggtc atcgggcctg
    89641 tggtgtcctg agagcctaca gaagtcattt tcggttcaca gtacgcgccc ggacgaatgc
    89701 ctttcacgcc acaggcacgg cctaccattt tctgcgccag cgagaagccg cgatcgctct
    89761 cagcgacatc tttcgcctga cggaacacat cactgtgcgg cagaccaagt gcttcacgcg
    89821 ctttggtggt caggccacga ccgataatca gcggaatacg gccgccagca cgcacttcat
    89881 caatcagcac gtcggttttc agttcgaagg tcgccagcag ttcgccggtt tcgtggttac
    89941 gtacttcacc tttgtacggg taaacgtcaa ttacgtcgcc catgttcagg ttagagacat
    90001 ccacttcgat tggcagcgca cccgcatctt ccatcgtgtt aaagaagatg ggtgcaattt
    90061 taccgccgag gcacaaacca ccgccgcgtt tgttcggcac atgtggaata tcatcgccca
    90121 taaaccacag aacggagttc gtggcggatt tacgcgatga acccgtaccc acaacgtcac
    90181 cgacgtacgc cagcgggaaa cctttctgtt gcagagcttc aatctgtttg atcgggccaa
    90241 caacacctgg ctggtctggc tcaatacctt cacgggcgtt tttcagcatc gccagcgcgt
    90301 gcaatgggat atccgggcgt gaccacgcat ccggtgccgg agaaaggtca tcggtgttgg
    90361 tttcgccagt gactttgaag acggtaacgg tcagtttttc agccagcgcc gggcgattca
    90421 ggaaccattc ggcatcagcc caggactgca taacctgctt cgcatattcg ttgcctgctt
    90481 tcgctttctc ttctacgtca tagaagttat cgaacatcag tagtgtgtga gaaagtgctt
    90541 tggcagcgat cggtgccagt ttggcatcat ccagcgcatc gatcagcgga tgaatgttgt
    90601 aaccaccctg catagtaccc agcagttcga tggctttttc cggagtcagc agtggggatt
    90661 tggcttcgcc tttggcgaca gcagccagga agcctgcttt aacataggcg gcttcatcga
    90721 cgccaggagg aacacggttg gttaacagat ctaacaggaa ttcttcttcg cccgcgggcg
    90781 ggtttttcag cagctctaca agcgcggcca tttggtttgc atccaggggt ttgggcgcaa
    90841 tcccctcagc ggcacgctca gctacgtgct tacggtattc ttctagcacg acggttctcc
    90901 tcgctctcat tgtcatagtg cggcaggcga ttctcttcac gctcctgtga gacagcagtt
    90961 tgtagggtaa atgcccagta ccgcaacggg cagaatagca ggattttgac gacgtgttaa
    91021 tctgtttaca aaaaagcaac ataaaaaagt ggctgaatcg ttaaggatgg tctagagatt
    91081 gtttccctcc gtaaaaactt cgttaattcg catggtgata atcacctttc tcaacgaatc
    91141 ccaagattag tcaaaattta aactaccgcc tctttatact cggattcaca gcacctgcgg
    91201 gtggcagttc gccgaccatt gcgatttcct tgagatccga attatgaaaa tgactttgcc
    91261 gtttaaaccc catgtgctgg cgctaatttg cagtgccggg ctttgtgccg cctctgccgg
    91321 gctatatata aaaagccgca cagtggaagc gcctgtggaa acgcaatcga cacaactggc
    91381 tgtgtctgac gctgccgcag ttacgcttcc tgcaacggtt tccgcacctc ccgtaacacc
    91441 cgccgtcgtc aaatccgcat tcagcactgc acaaatagat caatgggtcg cgcccgtcgc
    91501 gctgtatccc gacgccctac tttcgcaggt gctgatggca tcaacctatc cgacaaacgt
    91561 tgctcaagca gtgcaatggt cgcacgataa tccacttaaa caaggcgatg ctgctattca
    91621 ggcggtatct gaccagccgt gggacgccag cgttaaatca ctggtggcct ttccacaatt
    91681 gatggcattg atgggcgaaa acccgcaatg ggtgcaaaac ctgggcgatg cttttctggc
    91741 ccagccgcag gacgtgatgg actcggtaca acgattgcgg caactggcac aacaaaccgg
    91801 ctcgctgaag tcatcaaccg aacagaaagt tattaccaca acgaagaaaa ctgtaccggt
    91861 aacacagaca gtcacggctc ccgtcatacc atccaatacc gtttcaactg ccaaccctgt
    91921 cattacagag cctgcaacaa ccgtcatttc cattgagccc ggcaatcctg atgtggtcta
    91981 tattcccaac tacaacccaa ccgtggttta cgggaactgg gccaatactg cgtatccgcc
    92041 ggtttatctg ccaccaccag ccggagaacc gtttgttgac agctttgtac gcggattcgg
    92101 ctatagcatg ggcgttgcta ccacgtacgc actattcagc agcatcgact gggatgacga
    92161 cgatcatgac catcatcatc atgacgatga taattatcat caccacgatg gcggtcatcg
    92221 tgacggtaat ggctggcaac ataacggcga caacatcaat atcgacgtca acaatttcaa
    92281 ccgtatcacc ggtgagcatc ttactgataa gaatatggca tggcggcaca atccaaacta
    92341 ccgtaatggt gtgccctatc atgatcagga tatggcaaag cggtttcatc aaactgatgt
    92401 caacggcgga atgagtgcca cgcagctacc tgctccaaca cgcgacagcc agcgtcaggc
    92461 ggcagcaagt cagtttcagc aacgaacaca cgccgccccc gtcattacac gagataccca
    92521 acgtcaggca gcggcacagc ggtttaatga agctgaacac tatgggagct atgacgactt
    92581 ccgcgacttc agccgtcgcc aaccactgac ccagcaacaa aaggacgccg ctcgtcagcg
    92641 ttatcagtca gcttctcctg agcagcgcca ggcagttcac gagaaaatgc agactaaccc
    92701 gcagaaccag cagcgaagag aggcagcgcg tgagcgcatt cagcccgcct cgcctgagca
    92761 gcgccaggca gtccgcgaga aaatgcagac taacccacag atccagcagc gaagagacgc
    92821 agcgcgtgag cgtattcagt cagcctcgcc tgagcagcgc caggtgttta aggaaaaagt
    92881 acagcagcgc ccactgaacc aacagcaacg tgataacgcc cgccagcgtg ttcaatcagc
    92941 atcacctgaa caacgtcagg tttttcggga gaaagttcag gagagccgcc cacaacgtct
    93001 aaacgacagt aaccatactg ccaggctgaa taacgagcaa cggtcagcag tacgcgaacg
    93061 tctctctgag cgcggagcaa ggcgactgga aaggtaaatt acaggcgtaa aaaaagcggc
    93121 gtggttagcc gcttttttaa ttgccggatg ttccggcaaa cgaaaaatta cttcttcttc
    93181 gctttcgggt tcggcaggtc agtaatgcta ccttcgaaca cttctgccgc caggcccaca
    93241 gactcgtgca gagtcgggtg cgcgtggatg gtcagtgcga tgtcttcagc atcacaaccc
    93301 atttcaattg ccaggccgat ttcacccagc agctcgccgc cgttagtacc gacaatcgca
    93361 ccaccgatca cacggtgaga ttctttgtcg aaaatcagct tggtcatacc gtctgcgcag
    93421 tcggaagcga tagcacgacc agaagcagcc cacgggaagg tggcggtttc atagctgatg
    93481 cctttctctt tcgcttcttt ctcagtcaga cctacccatg caacttctgg ttcggtatag
    93541 gcgatggacg ggataacttt cggatcgaag tagtgtttct taccggcgat aacttcagcg
    93601 gcaacgtgac cttcgtgaac acctttgtgt gccagcatcg gctgaccgac gatatcgccg
    93661 atagcaaaga tgtgcggtac gttggtacgc agctgtttgt caacgcggat gaaaccacgg
    93721 tcgtcaactt ccacgccagc tttgcctgcg tcgaggtttt taccgttcgg cacacgacca
    93781 atcgctacca gcacggcgtc gtaacgctgc ggttcagctg gtgctttttt gccttccatc
    93841 gtcacataaa taccgtcttc tttcgcttca acggcggtaa ctttggtttc cagcatcagg
    93901 ttgaatttct tgctgatacg cttggtgaag actttaacga tgtctttgtc agctgccggg
    93961 ataacctggt cgaacatttc aaccacgtca atctgtgaac ccagcgcgtg gtaaacggtg
    94021 cccatttcca gaccgatgat accgccaccc attaccagca ggcgttctgg tacttctttc
    94081 agttccagcg cgtcagtaga gtcccagata cgcggatctt catgcggaat aaacggcagt
    94141 tgaatcgggc gagaacccgc tgcaatgatc gcgttgtcga agttgatcac ggttttaccg
    94201 ttctcacctt caacttccag ggtgttagcc ccggtaaatt tacccagacc gttgaccact
    94261 ttgactttgc ggcctttcgc cataccagcc agaccaccag tcagctgatt gatcactttc
    94321 tctttccagg tacgaatctt gtcgatatcg gttttcggtt cgccgaagac gataccgtgt
    94381 tcagccagcg ctttggcttc ttcgataact tttgctacgt gcagcagtgc tttagaaggg
    94441 atacagccga cgttcaggca aacaccgcca agggtgttgt aacgttctac gattacggtt
    94501 tccagaccta aatcagcgca acggaaggca gcggagtaac ctgcggggcc tgccccaagt
    94561 accacgacct gagttttgat ttcagtactc atcatgacct ctatatattt atctccggcg
    94621 ggtctgacgt tttttttatg acgcccatta tccaccgggt cgttctatcc gtcggtattt
    94681 tacaaaattg ttaacaattt ttaaacaaca aacggcaacc gatttgtcta ttcgctaata
    94741 acctcaatac attcatgaga ttaccagaaa aaagccggcc gttgggccgg ctcttttact
    94801 tacatcacca gacggcgaat gtcagacagc gtgttgttaa tgatggtaat gaaacgggca
    94861 ccatcagcac cgtcgatcac gcggtggtcg aaggagagag aaatcggcag catcagacgc
    94921 ggcacgaact ctttaccatt ccacaccggc tccatcgcgg acttggaaac gccgaggata
    94981 gccacttccg gcgcgttcac aatcggcgcg aagtgggtag tacccaggcc gccgatgctg
    95041 gagatggtga agcaaccgcc ctgcatttcg cccgcagtca gcttaccgtc acgcgctttc
    95101 ttagaaatag tcatcagctc gcgagacagt tcgatgatgc ctttcttgtt gacgtctttg
    95161 aataccggaa caaccagacc gttcggggta tccaccgcca caccgatgtt gatgtatttc
    95221 ttcagggtca gacgctgacc gtcttccgac agcgaactgt tgaagcgagg catctgctca
    95281 agcgctgcag caacggcttt catgatgaag acaaccgggg tgatcttcac atccagctta
    95341 cgtttcgccg cttcttcgtt ctgctgttta cggaacgctt ccagctcggt gatatcggtt
    95401 ttgtcgaagt gagtaacatg cgggatcatc acccagttac ggctcaggtt cgcaccagaa
    95461 attttctgga tacggcccag ttccacttct tcgatttcac caaacttgct gaagtccacc
    95521 ttcggccacg gcagcatgcc agggatacca ccgccagtcg ctgccggagc tgcttctgca
    95581 cgtttgatag cttctttcac gtaagcctga acgtcttcgc gcaggatacg acctttacgg
    95641 ccagtgccct tcactttcgc aaggttaacg ccaaactcgc gtgccagacg gcggatcagc
    95701 ggggtcgcgt gaacgtaagc gtcattttca gcaaattcag atttgccttc cgctttcgca
    95761 gccggtgctg ctgccggggc ttcagctttt gctgccggag ccggcgctgc tgcttcctgt
    95821 ttcgcaggag ctgccgcagg cgctgcgcct tcaacttcga agatcataat cagcgagcca
    95881 gttttcactt tatcgccaac gttgactttc agttccttca cgacgcctgc aaacggagcc
    95941 ggaacttcca tagaagcttt atcgccttct acggtaatca gtgactgttc agcggcaact
    96001 ttgtcgccca ctttcaccat cacttcggtc acttcaactt cgtcaccgcc gatatccgga
    96061 acgttaactt ctttcacgcc agccgctggt gcaggggccg ctgccggagc cgcttcctgt
    96121 ttagccgccg gagctgccgc gcctgcttca cccgccactt cgaagaccat aatcagcgag
    96181 ccggtagaca ctttgtcgcc ggtgttcact ttgatttctt tcacggtgcc tgcaaacgga
    96241 gccggaactt ccatagaagc tttatcgcct tctacggtga tcagcgactg ctcagcttca
    96301 actttgtcgc caactttcac caggatttcg gtcacttcaa cttcgtcgct gccgatatcc
    96361 ggaacgttaa cgtcttttgc cgcggcagcc gcggggactg ctgccggagc tgcttctttc
    96421 ttctcttctg cctgagcagg tgcagcgtct gctgcaccgt cggcggaatc gaaaatcata
    96481 atcagtgcgc cggtctgagt tttatcgcca acagagactt tgatctcttt aacgataccc
    96541 gcctgcggag acggaacttc catagaagct ttgtcgcctt ctacggtgat cagcgactgt
    96601 tcggcttcaa ctttgtcgcc cactttgacc aggatctcgg tgatttcaac ttcatcagcc
    96661 ccgatgtccg gtactttgat ttcgatagcc attattcttt tacctcttac gccagacgcg
    96721 ggttaacttt atctgcatcg atgttgaatt tggcgattgc gtcagcaacc actttcttat
    96781 cgatttcgcc acgtttagcc agttcgccca gcgccgcaac cacgacataa gaagcatcaa
    96841 cttcgaagtg gtgacgcagg ttctcacggc tgtcggaacg accgaagcca tcagtaccca
    96901 gtacgcggta gtcgtcagcc ggtacgtaag tacggacctg ctcagcgaac agtttcatat
    96961 agtcggtaga tgccacagcc ggagcgtcgt tcatcacctg agcgatatac ggaacgcgcg
    97021 gagtttccag cggatgcagc atgttccagc gttcacaatc ctgaccatca cgcgccagtt
    97081 cggtgaagga ggtcacgcta taaacgtcag aacctacgcc gtaatctttc gccaggatct
    97141 ctgccgcttc acggacgtga cgcaggatag aaccggagcc cagcagctga actttacctt
    97201 tgctaccttc aatggtttcg agtttgtaga tacctttacg gataccttcc tcagcacctt
    97261 ccggcattgc cggcatgtgg tagttttcgt tcagcgtggt gatgtagtag taaacgttct
    97321 cttgtttttc accgtacata cgctccagac cgtcatgcat aatgacagca acttcgtaag
    97381 cgtaagccgg gtcgtaagag atacagttcg gaatagtcag cgactgaatg tggctgtgac
    97441 catcttcgtg ctgcagacct tcaccgttca gggtggtacg accggaagta ccgccgatca
    97501 ggaagccacg cgcttgctgg tcgccagccg cccagcacag atcgccgata cgctggaagc
    97561 cgaacatcga gtaatagatg tagaacggaa tcatcggcag attgttggtg ctgtaagagg
    97621 tcgccgctgc cagccaggaa caacctgcgc ccagctcgtt gatcccttcc tgcagaatct
    97681 gacctttctc gtcttcttta tagtaagcaa cctgctcgcg gtcctgcggg gtgtactgct
    97741 gaccgttcgg gctgtaaata ccaatctgac ggaacagacc ttccataccg aaagtacgcg
    97801 cttcgtcggc gatgatcggt accagacgat ctttgatcga cttgttcttc agcatcacgt
    97861 tcagggcacg aacgaaagcg atagtggtag agatctcttt gctctgctct tccagcagcg
    97921 cgccgaagtc ttgcaggctt ggcagctcaa gcttctcggt gaagttcggc tgacggcttg
    97981 gcagataacc gtgcagtttc tgacgctgtg cgtgcagata ggtatgctct tcagaacctt
    98041 ccgggaaggt gatgtacggc agtttttcga tatctgcatc agacaccggc acattgaaac
    98101 ggtcgcggat gtgacgcaca ccgtccatgt tcattttctt aacctggtgc gcgatgtttt
    98161 taccttcagc cgcgtcgccc atgccgtaac ctttaatggt atgagcaagg attaccgtcg
    98221 ctttgccttt ggtttcctgc gctttcttga atgcagcgta gattttcttc ggatcgtgac
    98281 caccacggtt cagtgcccag atctgctcgt cagtccagtc tgcaaccagt gctgcggttt
    98341 caggatattt accgaagaag tgttcacgaa cgtacgcacc atctttcgat ttgaaggtct
    98401 ggtagtcgcc gtcaacggtt tcgttcatca gctggatcag tttaccgctg gtatctttac
    98461 gcagcagttc atcccaacgg ctaccccaca tcactttgat cacgttccag ccagcacctt
    98521 cgaagatacc ttccagttcg ttgatgatct tgccgttacc ggtgaccggg ccgtcaagac
    98581 gctgcaggtt acagttgata acgaagacca ggttatccag tttttcacgg gtagcgatgg
    98641 tgatcgcacc tttggattcc ggttcgtcca tttcaccgtc gccgaggaac gcgtaaacgg
    98701 tctgtttaga ggtatctttc aggccacggt gttccagata tttcaggaat ttagcctggt
    98761 aaatagcacc aatcggaccc agacccatag atacggtcgg gaactgccag aattccggca
    98821 tcaatttcgg gtgcggatag gaagagaggc cattgccgtg aacttcctga cggaagttat
    98881 ccagctgctc ttgagtcaga cgaccttcca ggaaagcacg agcgtacacg cccggggaga
    98941 tgtggccctg gaagtaaacc aggtcgccgc catcctgctc gttgcgtgca cggaagaagt
    99001 ggttaaagca cacatcataa atggttgcgg aagactggaa ggacgccatg tggccgccca
    99061 gttcgaggtc ttttttcgac gcacgcagaa ccgtcatgat ggcgttccag cggatagctg
    99121 aacgaatacg gcgttccagt tccagattac ccggatactc cggttgttct tcaacgggga
    99181 tggtgttgat gtagttgctg ataccggtgc ctgcggctac gttgacaccg cctttgcggg
    99241 cttcagcaag cagttggtcg atcagatact gagcacgctc aacaccttct tcacggatga
    99301 ccgattcgat cgcctggagc cagtcgcgag tttcgatcgg atccacgtca tttgggaaac
    99361 gttctgacat gggttattcc ttatctatct aataacgttg agttttctgg aacctgtccc
    99421 attgaactct cgccggaaag ctcaataaga caggttctac gtttagttgc cgcgctttta
    99481 tatgcgcttg atttacaaca tcttctggat aatttttacc agaaaaatca ctaattcttt
    99541 cgttgctcca gacgacgcag agaacgctca cggcggcttt cttcacggct tctgtcgagc
    99601 aaaatttctt cgataaaggc cagatggcga tgcgatgctt cgcgcgcttc ttccggctta
    99661 ccggccataa tcgcttcaaa tatgcgggtg cggtgactac tcaccagcgg cagcatctcg
    99721 cgacgcgaat agagcaattc gaagttctgg cggacattct gggccaacat cggctccata
    99781 caccttagca gatgaagcag aaccacattg tgggccgctt cggtgacggc aatctgatac
    99841 tggagtacgg cgtttgattc cgcgtccaga tcgccagact gctgcgccag ttctatggcg
    99901 tggtggagtt cacggatgcg ttccttgtct tcatcggtac tacgcagcgc ggcgtaataa
    99961 gcggcgatac cttccagggc gtgtcgtgtt tcgagcaagt catactgtga ttcaggatgg
   100021 tcggagagca gctccaccag cggatcgctg aagctttgcc ataggctgct ctggacaaaa
   100081 gtgccgccac cctggcgacg aagcaacaag cccttcgctt cgagacgttg aatcgcctca
   100141 cgcaatgagg gacgggagac gtcaaactgt tttgccagtt cgcgttccgg tgggagtttt
   100201 tcgcccgggc ggagagtgcc ttcgaggatc aaaaactcca gttgctgctc aatcacatcg
   100261 gagagttttg gttggcggat tttgctgtag gccatgagtt cctgtcttaa gccacttgcc
   100321 gaagtcaatt ggtcttacca atttcatgtc tgtgacgcta aagtaacaaa gtattcacct
   100381 tatgtccata caggttttga ttgaaatcat gaaactgtgc acattttaac aact
//
LOCUS       DAZKIP010000003       100389 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZKIP010000003 DAZKIP010000000
VERSION     DAZKIP010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 100389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 100389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..100389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.003"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(95..835)
                     /gene="cysQ"
                     /locus_tag="ACHOUK_000193"
     CDS             complement(95..835)
                     /gene="cysQ"
                     /locus_tag="ACHOUK_000193"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFP9895725.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGAKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            1025..2968
                     /gene="cpdB"
                     /locus_tag="ACHOUK_000194"
     CDS             1025..2968
                     /gene="cpdB"
                     /locus_tag="ACHOUK_000194"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFP9895726.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINNARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYEPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(3086..3466)
                     /gene="ytfH"
                     /locus_tag="ACHOUK_000195"
     CDS             complement(3086..3466)
                     /gene="ytfH"
                     /locus_tag="ACHOUK_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFP9895727.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            3555..4415
                     /gene="qorB"
                     /locus_tag="ACHOUK_000196"
     CDS             3555..4415
                     /gene="qorB"
                     /locus_tag="ACHOUK_000196"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313217.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFP9895728.1"
                     /translation="MIAITGATGQLGQYVIESLMKTVPASQIVAIVRNPAKAQALTAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKTAGVKFIAYT
                     SLLHADKSPLGLADEHIETEKMLADSGIVYTLLRNGWYTENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGHPTTTLAE
                     SVSHLFNVNN"
     gene            4523..5488
                     /gene="ytfF"
                     /locus_tag="ACHOUK_000197"
     CDS             4523..5488
                     /gene="ytfF"
                     /locus_tag="ACHOUK_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFP9895729.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLACVNIAELNHGLPNFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFISLMIAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            5596..6258
                     /gene="ytfE"
                     /locus_tag="ACHOUK_000198"
     CDS             5596..6258
                     /gene="ytfE"
                     /locus_tag="ACHOUK_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFP9895730.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHARHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHNTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(6303..7715)
                     /gene="cycA"
                     /locus_tag="ACHOUK_000199"
                     /gene_synonym="dagA"
     CDS             complement(6303..7715)
                     /gene="cycA"
                     /locus_tag="ACHOUK_000199"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFP9895731.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(8024..8644)
                     /gene="fklB"
                     /locus_tag="ACHOUK_000200"
     CDS             complement(8024..8644)
                     /gene="fklB"
                     /locus_tag="ACHOUK_000200"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFP9895732.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            8862..9500
                     /gene="ytfB"
                     /locus_tag="ACHOUK_000201"
     CDS             8862..9500
                     /gene="ytfB"
                     /locus_tag="ACHOUK_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFP9895733.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNYPDQVAPVAPEPIQ
                     EGQPEEQPKTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            complement(9484..9591)
                     /locus_tag="ACHOUK_000202"
                     /pseudo
     CDS             complement(9484..9591)
                     /locus_tag="ACHOUK_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(9635..10843)
                     /locus_tag="ACHOUK_000203"
     CDS             complement(9635..10843)
                     /locus_tag="ACHOUK_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFP9895734.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTASQSCGKWY
                     ISIQTENEVSTPVHPSALMVGLDAGVAKLATLSDGTVFGPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQACGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQ
                     EPTEMIQATA"
     gene            10851..11282
                     /gene="tnpA"
                     /locus_tag="ACHOUK_000204"
     CDS             10851..11282
                     /gene="tnpA"
                     /locus_tag="ACHOUK_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element IS609 family
                     transposase"
                     /protein_id="HFP9895735.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTRYRRQIFDHDATEK
                     LRTYFSKVCADFEAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGVSGRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCGGAPISAIRQYIEQQQTPG"
     gene            complement(11340..11633)
                     /locus_tag="ACHOUK_000205"
                     /pseudo
     CDS             complement(11340..11633)
                     /locus_tag="ACHOUK_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            11905..12699
                     /gene="yjfZ"
                     /locus_tag="ACHOUK_000206"
     CDS             11905..12699
                     /gene="yjfZ"
                     /locus_tag="ACHOUK_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFP9895736.1"
                     /translation="MTLPTTIYSFPAYLSRFSSTDKPVKLKFHQYARATLLSNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLATKCNAYDGVTMNGDNSFGWNHEATLDNIHAQKY
                     NKAYAGARLTAELKYLLQDVESFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPA
                     QMPNCWSSELPTIDLYIDQLRTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSSS
                     AAIYSGLGFERVPQERDISCARMYLTPANHPDLWTQENGEWIYLRN"
     gene            complement(12770..13219)
                     /gene="rplI"
                     /locus_tag="ACHOUK_000207"
     CDS             complement(12770..13219)
                     /gene="rplI"
                     /locus_tag="ACHOUK_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFP9895737.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(13261..13488)
                     /gene="rpsR"
                     /locus_tag="ACHOUK_000208"
     CDS             complement(13261..13488)
                     /gene="rpsR"
                     /locus_tag="ACHOUK_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFP9895738.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(13493..13807)
                     /gene="priB"
                     /locus_tag="ACHOUK_000209"
     CDS             complement(13493..13807)
                     /gene="priB"
                     /locus_tag="ACHOUK_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFP9895739.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(13814..14209)
                     /gene="rpsF"
                     /locus_tag="ACHOUK_000210"
     CDS             complement(13814..14209)
                     /gene="rpsF"
                     /locus_tag="ACHOUK_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFP9895740.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            14536..14811
                     /gene="yjfY"
                     /locus_tag="ACHOUK_000211"
     CDS             14536..14811
                     /gene="yjfY"
                     /locus_tag="ACHOUK_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFP9895741.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(14940..15626)
                     /gene="ulaF"
                     /locus_tag="ACHOUK_000212"
     CDS             complement(14940..15626)
                     /gene="ulaF"
                     /locus_tag="ACHOUK_000212"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFP9895742.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(15626..16480)
                     /gene="ulaE"
                     /locus_tag="ACHOUK_000213"
     CDS             complement(15626..16480)
                     /gene="ulaE"
                     /locus_tag="ACHOUK_000213"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFP9895743.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            complement(16490..17140)
                     /gene="ulaD"
                     /locus_tag="ACHOUK_000214"
     CDS             complement(16490..17140)
                     /gene="ulaD"
                     /locus_tag="ACHOUK_000214"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFP9895744.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(17154..17618)
                     /gene="ulaC"
                     /locus_tag="ACHOUK_000215"
     CDS             complement(17154..17618)
                     /gene="ulaC"
                     /locus_tag="ACHOUK_000215"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFP9895745.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQEAVKIGVDLLVAADVVEPRYY
                     QAILDGVEQFGPYFVIAPGLAMPHGRPEEGVKKTGFSLVTLKKPLEFNHDDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(17628..17933)
                     /gene="ulaB"
                     /locus_tag="ACHOUK_000216"
     CDS             complement(17628..17933)
                     /gene="ulaB"
                     /locus_tag="ACHOUK_000216"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFP9895746.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            complement(17949..19346)
                     /gene="ulaA"
                     /locus_tag="ACHOUK_000217"
     CDS             complement(17949..19346)
                     /gene="ulaA"
                     /locus_tag="ACHOUK_000217"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFP9895747.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIILGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            19701..20765
                     /gene="ulaG"
                     /locus_tag="ACHOUK_000218"
                     /pseudo
     CDS             19701..20765
                     /gene="ulaG"
                     /locus_tag="ACHOUK_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
     gene            20873..21628
                     /gene="ulaR"
                     /locus_tag="ACHOUK_000219"
     CDS             20873..21628
                     /gene="ulaR"
                     /locus_tag="ACHOUK_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFP9895748.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(21625..22374)
                     /gene="yjfP"
                     /locus_tag="ACHOUK_000220"
     CDS             complement(21625..22374)
                     /gene="yjfP"
                     /locus_tag="ACHOUK_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFP9895749.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEK
                     WLLDDRLAVGGASMGAMTALGITARHPTVRCTASMMGSGYFTSLARLLFPPLIPETVA
                     QQNEFNNIVAPLAEWEATNHLEQLSDRPLLLWYGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            22556..22885
                     /gene="bsmA"
                     /locus_tag="ACHOUK_000221"
     CDS             22556..22885
                     /gene="bsmA"
                     /locus_tag="ACHOUK_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFP9895750.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            23034..23309
                     /gene="yjfN"
                     /locus_tag="ACHOUK_000222"
     CDS             23034..23309
                     /gene="yjfN"
                     /locus_tag="ACHOUK_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFP9895751.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(23426..25051)
                     /gene="aidB"
                     /locus_tag="ACHOUK_000223"
     CDS             complement(23426..25051)
                     /gene="aidB"
                     /locus_tag="ACHOUK_000223"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFP9895752.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDCAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(25135..26298)
                     /gene="yjfC"
                     /locus_tag="ACHOUK_000224"
     CDS             complement(25135..26298)
                     /gene="yjfC"
                     /locus_tag="ACHOUK_000224"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFP9895753.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(26301..26939)
                     /gene="yjfM"
                     /locus_tag="ACHOUK_000225"
     CDS             complement(26301..26939)
                     /gene="yjfM"
                     /locus_tag="ACHOUK_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFP9895754.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(26949..27347)
                     /gene="yjfL"
                     /locus_tag="ACHOUK_000226"
     CDS             complement(26949..27347)
                     /gene="yjfL"
                     /locus_tag="ACHOUK_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFP9895755.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(27365..28024)
                     /gene="yjfK"
                     /locus_tag="ACHOUK_000227"
     CDS             complement(27365..28024)
                     /gene="yjfK"
                     /locus_tag="ACHOUK_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFP9895756.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVVAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKENHWREAINAKTMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(28075..28773)
                     /gene="yjfJ"
                     /locus_tag="ACHOUK_000228"
     CDS             complement(28075..28773)
                     /gene="yjfJ"
                     /locus_tag="ACHOUK_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFP9895757.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARTLEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVAATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(28792..29193)
                     /gene="yjfI"
                     /locus_tag="ACHOUK_000229"
     CDS             complement(28792..29193)
                     /gene="yjfI"
                     /locus_tag="ACHOUK_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HFP9895758.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNSENALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALS
                     LKSSLEDILLEITSLVDNALDLAEITEEYSH"
     gene            complement(29320..30051)
                     /gene="rlmB"
                     /locus_tag="ACHOUK_000230"
     CDS             complement(29320..30051)
                     /gene="rlmB"
                     /locus_tag="ACHOUK_000230"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFP9895759.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(30232..32673)
                     /gene="rnr"
                     /locus_tag="ACHOUK_000231"
     CDS             complement(30232..32673)
                     /gene="rnr"
                     /locus_tag="ACHOUK_000231"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFP9895760.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLMLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVSDWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(32712..33137)
                     /gene="nsrR"
                     /locus_tag="ACHOUK_000232"
     CDS             complement(32712..33137)
                     /gene="nsrR"
                     /locus_tag="ACHOUK_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFP9895761.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(33342..34640)
                     /gene="purA"
                     /locus_tag="ACHOUK_000233"
     CDS             complement(33342..34640)
                     /gene="purA"
                     /locus_tag="ACHOUK_000233"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFP9895762.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(34744..34941)
                     /gene="yjeT"
                     /locus_tag="ACHOUK_000234"
     CDS             complement(34744..34941)
                     /gene="yjeT"
                     /locus_tag="ACHOUK_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFP9895763.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(35023..36027)
                     /gene="hflC"
                     /locus_tag="ACHOUK_000235"
     CDS             complement(35023..36027)
                     /gene="hflC"
                     /locus_tag="ACHOUK_000235"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFP9895764.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(36030..37289)
                     /gene="hflK"
                     /locus_tag="ACHOUK_000236"
     CDS             complement(36030..37289)
                     /gene="hflK"
                     /locus_tag="ACHOUK_000236"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFP9895765.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(37375..38655)
                     /gene="hflX"
                     /locus_tag="ACHOUK_000237"
     CDS             complement(37375..38655)
                     /gene="hflX"
                     /locus_tag="ACHOUK_000237"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFP9895766.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(38732..39040)
                     /gene="hfq"
                     /locus_tag="ACHOUK_000238"
     CDS             complement(38732..39040)
                     /gene="hfq"
                     /locus_tag="ACHOUK_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFP9895767.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(39126..40076)
                     /gene="miaA"
                     /locus_tag="ACHOUK_000239"
     CDS             complement(39126..40076)
                     /gene="miaA"
                     /locus_tag="ACHOUK_000239"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFP9895768.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEIDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLGGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(40069..41916)
                     /gene="mutL"
                     /locus_tag="ACHOUK_000240"
     CDS             complement(40069..41916)
                     /gene="mutL"
                     /locus_tag="ACHOUK_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFP9895769.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRPIPENRVAAGRNHFAEPAVREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(41926..43263)
                     /gene="amiB"
                     /locus_tag="ACHOUK_000241"
     CDS             complement(41926..43263)
                     /gene="amiB"
                     /locus_tag="ACHOUK_000241"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFP9895770.1"
                     /translation="MMYRIRNWLVATLLLLCAQVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVVKRVETPTVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANNGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(43282..43743)
                     /gene="tsaE"
                     /locus_tag="ACHOUK_000242"
     CDS             complement(43282..43743)
                     /gene="tsaE"
                     /locus_tag="ACHOUK_000242"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFP9895771.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(43715..45262)
                     /gene="nnr"
                     /locus_tag="ACHOUK_000243"
     CDS             complement(43715..45262)
                     /gene="nnr"
                     /locus_tag="ACHOUK_000243"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFP9895772.1"
                     /translation="MTDHTMKKNPVSIPHTVWHADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVALIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            45261..46400
                     /gene="queG"
                     /locus_tag="ACHOUK_000244"
     CDS             45261..46400
                     /gene="queG"
                     /locus_tag="ACHOUK_000244"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFP9895773.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAMAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(46383..46436)
                     /locus_tag="ACHOUK_000245"
     CDS             complement(46383..46436)
                     /locus_tag="ACHOUK_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9895774.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(46670..46745)
                     /locus_tag="ACHOUK_000246"
     tRNA            complement(46670..46745)
                     /locus_tag="ACHOUK_000246"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46710..46712),aa:Gly,seq:gcc)
     gene            complement(46782..46857)
                     /locus_tag="ACHOUK_000247"
     tRNA            complement(46782..46857)
                     /locus_tag="ACHOUK_000247"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46822..46824),aa:Gly,seq:gcc)
     gene            complement(47068..47613)
                     /gene="orn"
                     /locus_tag="ACHOUK_000248"
     CDS             complement(47068..47613)
                     /gene="orn"
                     /locus_tag="ACHOUK_000248"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFP9895775.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDGWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            47708..48760
                     /gene="rsgA"
                     /locus_tag="ACHOUK_000249"
     CDS             47708..48760
                     /gene="rsgA"
                     /locus_tag="ACHOUK_000249"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFP9895776.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            48857..49825
                     /gene="asd"
                     /locus_tag="ACHOUK_000250"
     CDS             48857..49825
                     /gene="asd"
                     /locus_tag="ACHOUK_000250"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFP9895777.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            49847..53170
                     /gene="mscM"
                     /locus_tag="ACHOUK_000251"
     CDS             49847..53170
                     /gene="mscM"
                     /locus_tag="ACHOUK_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFP9895778.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(53321..54823)
                     /gene="yjeM"
                     /locus_tag="ACHOUK_000252"
     CDS             complement(53321..54823)
                     /gene="yjeM"
                     /locus_tag="ACHOUK_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFP9895779.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(55042..56019)
                     /gene="epmA"
                     /locus_tag="ACHOUK_000253"
     CDS             complement(55042..56019)
                     /gene="epmA"
                     /locus_tag="ACHOUK_000253"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFP9895780.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            56344..58152
                     /gene="frdA"
                     /locus_tag="ACHOUK_000254"
     CDS             56344..58152
                     /gene="frdA"
                     /locus_tag="ACHOUK_000254"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFP9895781.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTKRDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            58145..58879
                     /gene="frdB"
                     /locus_tag="ACHOUK_000255"
     CDS             58145..58879
                     /gene="frdB"
                     /locus_tag="ACHOUK_000255"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFP9895782.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            58890..59285
                     /gene="frdC"
                     /locus_tag="ACHOUK_000256"
     CDS             58890..59285
                     /gene="frdC"
                     /locus_tag="ACHOUK_000256"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFP9895783.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            59296..59655
                     /gene="frdD"
                     /locus_tag="ACHOUK_000257"
     CDS             59296..59655
                     /gene="frdD"
                     /locus_tag="ACHOUK_000257"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFP9895784.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            59718..60851
                     /gene="ampC"
                     /locus_tag="ACHOUK_000258"
     CDS             59718..60851
                     /gene="ampC"
                     /locus_tag="ACHOUK_000258"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFP9895785.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIVNGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            60939..61472
                     /gene="blc"
                     /locus_tag="ACHOUK_000259"
     CDS             60939..61472
                     /gene="blc"
                     /locus_tag="ACHOUK_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFP9895786.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(61469..61786)
                     /gene="sugE"
                     /locus_tag="ACHOUK_000260"
     CDS             complement(61469..61786)
                     /gene="sugE"
                     /locus_tag="ACHOUK_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFP9895787.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(61962..62108)
                     /gene="ecnB"
                     /locus_tag="ACHOUK_000261"
     CDS             complement(61962..62108)
                     /gene="ecnB"
                     /locus_tag="ACHOUK_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFP9895788.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(62219..62344)
                     /gene="ecnA"
                     /locus_tag="ACHOUK_000262"
     CDS             complement(62219..62344)
                     /gene="ecnA"
                     /locus_tag="ACHOUK_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFP9895789.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(62396..62962)
                     /gene="efp"
                     /locus_tag="ACHOUK_000263"
     CDS             complement(62396..62962)
                     /gene="efp"
                     /locus_tag="ACHOUK_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFP9895790.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            63004..64032
                     /gene="epmB"
                     /locus_tag="ACHOUK_000264"
     CDS             63004..64032
                     /gene="epmB"
                     /locus_tag="ACHOUK_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFP9895791.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RNAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRAGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            64267..65136
                     /gene="yjeJ"
                     /locus_tag="ACHOUK_000265"
     CDS             64267..65136
                     /gene="yjeJ"
                     /locus_tag="ACHOUK_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFP9895792.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSP
                     LDFLPLYDVDCQQNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLVGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(65328..65681)
                     /gene="yjeI"
                     /locus_tag="ACHOUK_000266"
     CDS             complement(65328..65681)
                     /gene="yjeI"
                     /locus_tag="ACHOUK_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFP9895793.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(65819..67465)
                     /gene="groL"
                     /locus_tag="ACHOUK_000267"
     CDS             complement(65819..67465)
                     /gene="groL"
                     /locus_tag="ACHOUK_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFP9895794.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(67509..67802)
                     /gene="groS"
                     /locus_tag="ACHOUK_000268"
     CDS             complement(67509..67802)
                     /gene="groS"
                     /locus_tag="ACHOUK_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFP9895795.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            68078..69334
                     /gene="yjeH"
                     /locus_tag="ACHOUK_000269"
     CDS             68078..69334
                     /gene="yjeH"
                     /locus_tag="ACHOUK_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFP9895796.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPILIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(69350..69826)
                     /gene="fxsA"
                     /locus_tag="ACHOUK_000270"
     CDS             complement(69350..69826)
                     /gene="fxsA"
                     /locus_tag="ACHOUK_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFP9895797.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            70163..71599
                     /gene="aspA"
                     /locus_tag="ACHOUK_000271"
     CDS             70163..71599
                     /gene="aspA"
                     /locus_tag="ACHOUK_000271"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFP9895798.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            71717..73018
                     /gene="dcuA"
                     /locus_tag="ACHOUK_000272"
     CDS             71717..73018
                     /gene="dcuA"
                     /locus_tag="ACHOUK_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFP9895799.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            73134..73472
                     /gene="cutA"
                     /locus_tag="ACHOUK_000273"
     CDS             73134..73472
                     /gene="cutA"
                     /locus_tag="ACHOUK_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFP9895800.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            73448..75145
                     /gene="dsbD"
                     /locus_tag="ACHOUK_000274"
     CDS             73448..75145
                     /gene="dsbD"
                     /locus_tag="ACHOUK_000274"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFP9895801.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPVDQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLLTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFIMGTIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            75182..75757
                     /gene="yjdC"
                     /locus_tag="ACHOUK_000275"
     CDS             75182..75757
                     /gene="yjdC"
                     /locus_tag="ACHOUK_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9895802.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            75864..75939
                     /locus_tag="ACHOUK_000276"
     tRNA            75864..75939
                     /locus_tag="ACHOUK_000276"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:75897..75899,aa:Phe,seq:gaa)
     gene            76137..76296
                     /locus_tag="ACHOUK_000277"
                     /pseudo
     CDS             76137..76296
                     /locus_tag="ACHOUK_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            76556..78094
                     /gene="cadC"
                     /locus_tag="ACHOUK_000278"
     CDS             76556..78094
                     /gene="cadC"
                     /locus_tag="ACHOUK_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFP9895803.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSSLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            78460..79794
                     /gene="cadB"
                     /locus_tag="ACHOUK_000279"
     CDS             78460..79794
                     /gene="cadB"
                     /locus_tag="ACHOUK_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFP9895804.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            79874..82021
                     /gene="cadA"
                     /locus_tag="ACHOUK_000280"
     CDS             79874..82021
                     /gene="cadA"
                     /locus_tag="ACHOUK_000280"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFP9895805.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            82080..83537
                     /gene="dtpC"
                     /locus_tag="ACHOUK_000281"
     CDS             82080..83537
                     /gene="dtpC"
                     /locus_tag="ACHOUK_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFP9895806.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESH
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMVSGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMVQESND"
     gene            83774..85291
                     /gene="lysS"
                     /locus_tag="ACHOUK_000282"
     CDS             83774..85291
                     /gene="lysS"
                     /locus_tag="ACHOUK_000282"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFP9895807.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(85410..85583)
                     /gene="ghoT"
                     /locus_tag="ACHOUK_000283"
     CDS             complement(85410..85583)
                     /gene="ghoT"
                     /locus_tag="ACHOUK_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFP9895808.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(85611..85907)
                     /gene="ghoS"
                     /locus_tag="ACHOUK_000284"
     CDS             complement(85611..85907)
                     /gene="ghoS"
                     /locus_tag="ACHOUK_000284"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFP9895809.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            complement(86134..86406)
                     /gene="yjdJ"
                     /locus_tag="ACHOUK_000285"
     CDS             complement(86134..86406)
                     /gene="yjdJ"
                     /locus_tag="ACHOUK_000285"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9895810.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            complement(86418..86648)
                     /gene="yjdI"
                     /locus_tag="ACHOUK_000286"
     CDS             complement(86418..86648)
                     /gene="yjdI"
                     /locus_tag="ACHOUK_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFP9895811.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            86829..88460
                     /gene="dcuS"
                     /locus_tag="ACHOUK_000287"
     CDS             86829..88460
                     /gene="dcuS"
                     /locus_tag="ACHOUK_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP9895812.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            88457..89176
                     /gene="dcuR"
                     /locus_tag="ACHOUK_000288"
     CDS             88457..89176
                     /gene="dcuR"
                     /locus_tag="ACHOUK_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFP9895813.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            89747..91087
                     /gene="dcuB"
                     /locus_tag="ACHOUK_000289"
     CDS             89747..91087
                     /gene="dcuB"
                     /locus_tag="ACHOUK_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFP9895814.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            91165..92811
                     /gene="fumB"
                     /locus_tag="ACHOUK_000290"
     CDS             91165..92811
                     /gene="fumB"
                     /locus_tag="ACHOUK_000290"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFP9895815.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSVETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHHPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIINKQCANCTK"
     gene            92934..93563
                     /gene="yjdF"
                     /locus_tag="ACHOUK_000291"
     CDS             92934..93563
                     /gene="yjdF"
                     /locus_tag="ACHOUK_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFP9895816.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(93702..95123)
                     /gene="melB"
                     /locus_tag="ACHOUK_000292"
     CDS             complement(93702..95123)
                     /gene="melB"
                     /locus_tag="ACHOUK_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFP9895817.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIAFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKIPPEPVHADIPVGAVSDVKA"
     gene            complement(95226..96581)
                     /gene="melA"
                     /locus_tag="ACHOUK_000293"
     CDS             complement(95226..96581)
                     /gene="melA"
                     /locus_tag="ACHOUK_000293"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFP9895818.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYAPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYDAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            96864..97772
                     /gene="melR"
                     /locus_tag="ACHOUK_000294"
     CDS             96864..97772
                     /gene="melR"
                     /locus_tag="ACHOUK_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFP9895819.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACIPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            97971..100238
                     /gene="adiA"
                     /locus_tag="ACHOUK_000295"
     CDS             97971..100238
                     /gene="adiA"
                     /locus_tag="ACHOUK_000295"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFP9895820.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFAADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT        24900 a        26355 c        24675 g        24459 t
ORIGIN      
        1 actttaaata caatctcttt ttatttataa ctttttaggt tgggagaaat caggatggca
       61 gaatcaggaa atacactgtt tctgccatct gaatttagta aatagacact ctgaaccccg
      121 gattcaggaa cgactcgcgc ggagtgtaat ccagcggttt accctgccag tcgtgaacgt
      181 gcgctccggc agctgcagcc acagcatgtc cagcggcggt gtcccaaata ttcgttggtc
      241 cgaaacgcgg gtacagctgc gcctgtcctt ccgccaccag gcagaacttc agcgaagagc
      301 cgatggacgt ggtctgatgt tcgccaagct gttgcagata ctctttcagc tccgcatccg
      361 catgagagcg gctgatcacc accagcggcg ggcgcgcatc gcggacctga atctgcttgc
      421 gcacgccgca ctcttctttc caggccttgc cttctgccgc gctgtacatt acgttcatta
      481 ccggcgcata caccacacct aaaatcggtt tgccatggtc aatgagcgca atgttaacgg
      541 tgaattcgcc attacgttta ataaactctt tcgtaccatc cagcgggtct accagccagt
      601 aacgctgcca gtgctgacgg acttcccaac cgggaggatc ttcttcagaa aggaccggaa
      661 tatccggtgt cagcgtacgt aaaccgtcca tgataacggt gtgagcggca atatccgctg
      721 ccgttaccgg agaattgtcc gctttgctga cgacgtccat cggtttcgcc ccgtcgtaga
      781 cctgcataat ggcatcgcct gcattccgtg caagctggca tacttgatct aacatttctc
      841 cacctcgtct ctgtgagcgg tgttaactta ttgttttact tataccctat cattaatgaa
      901 tgcgccaact gtgatagtgt catcattttc aaagcgtaaa attgtggcat tcttcactgt
      961 tctataagta agacgtttat tctccctttt ctttcgtatt cccgatgata aaaggatgtc
     1021 cctgatgatt aagtttagcg caacgctcct ggccacgctg attgccgcca gtgtgaatgc
     1081 agcgacggtc gatctgcgta tcatggaaac cactgatctg catagcaaca tgatggattt
     1141 cgattattac aaagacaccg ccacggaaaa attcggactg gtacgtacgg caagcctgat
     1201 taacaatgcc cgcaatgaag tgaaaaacag cgtactggtc gataacggcg atttgattca
     1261 ggggagtccg ctggccgatt acatgtcggc gaaaggatta aaagcaggtg atattcaccc
     1321 ggtctataag gcattaaata cgctggacta taccgtcgga acgcttggca accacgagtt
     1381 taactacggt ctggattacc tgaaaaatgc gctggcagga gcgaaattcc cttatgtaaa
     1441 tgccaacgtc attgacgcca gaaccaaaca gccaatgttt acaccgtatt taattaaaga
     1501 caccgaagtg gtcgataaag acggaaaaaa acagacgctg aagattggct atattggcgt
     1561 cgtgccgccg caaatcatgg gctgggataa agctaattta tccggaaaag tgacggtgaa
     1621 tgatattacc gaaaccgtgc gcaaatacga gcctgaaatg cgcgagaaag gtgccgatgt
     1681 cgttgtcgtt ctggcgcatt ccgggctgtc tgccgatccg tataaagtaa tggcggaaaa
     1741 ctcagtttat tacctcagtg aaattccggg cgttaacgcc attatgtttg gccatgctca
     1801 cgccgttttc ccgggtaaag attttgctga tatcgaaggg gctgatatca ccaaaggcac
     1861 gctgaatggt gttccggcgg taatgccggg catgtggggc gatcatcttg gggtggtcga
     1921 cttacaactc agtaatgaca gcggtaaatg gcaggtgacg caggcgaaag cggaagcacg
     1981 gccgatttac gacatcgcca ataaaaaatc cctcgcggcg gaagacagca agctggtaga
     2041 aacactcaaa gccgatcacg atgccacacg ccagttcgtc agcaagccaa tcggtaaatc
     2101 tgccgacaat atgtatagct atctggcgct ggtgcaggac gatccgaccg tgcaagtagt
     2161 gaacaacgcg caaaaagcgt atgtcgagca ttacattcag ggcgatccgg atctggcaaa
     2221 actgccggtg ctttcagctg ccgcaccgtt taaagtcggt ggtcgcaaaa atgacccggc
     2281 aagctatgtg gaggtggaaa aaggccagtt gaccttccgt aatgccgccg atctttatct
     2341 ctatcccaat acgctgattg tggtgaaagc cagcggtaaa gaggtgaaag agtggctgga
     2401 gtgctccgcc ggacagttta accagattga tcccaacagc acgaaaccac agtcactcat
     2461 caactgggat ggtttccgca cttataactt tgatgttatt gatggtgtga attatcagat
     2521 tgatgttacc cagcccgccc gttatgacgg cgagtgccag atgattaatg ccaatgcgga
     2581 aaggattaag aacctgacct ttaatggcaa gccgattgat ccgaacgcta tgttcctcgt
     2641 tgccaccaat aactatcgcg cttacggcgg caaatttgcc gggacgggcg acagccatat
     2701 cgcttttgct tcaccggatg agaaccgctc ggtgctggca gcgtggattg ctgatgagtc
     2761 gaaacgtgcg ggggaaattc acccggcggc agataacaac tggcgtttag caccgatagc
     2821 tgccgataag aaactggata tccgtttcga aacttccccg tcagataaag ccgcagcgtt
     2881 tattaaagag aaagggcagt atccgatgaa taaagtcgcg accgatgata tcgggtttgc
     2941 gatttatcag gtggatttga gtaagtaaaa cacttctttt tcggcctata aatcatcaat
     3001 cgcatccggc atttattggc ggatgcgatg ctggcgcatc ttatccgccc tacaagtcat
     3061 acaccgtagg ccgcgcatcc ggcagttatg ccgcacgttc atcccgcacc gccaacacct
     3121 cgggcaaatt caactcaatc cagtccgcca gtgcagcaac cttttcgctc acctgctcgc
     3181 ccagcggcgt gaggctgtat tcaacatgcg gcggcaccac cggatacgcg atacggttaa
     3241 gaaaaccatc ctgttctaac gcctgtaacg actgcgcaag catcttttcg ctcaccccgc
     3301 cgattttgcg ccgcaggtcg ctaaagcgat gagtaccttc gcgtagcgcc accagaatca
     3361 acacccccca acggctggtg acgtgtttca acacctcgcg cgacgggcac tgttccgcaa
     3421 agagattacc ctctttcagt tgttgcgaca ggctaacctg actcatttca tacttacctt
     3481 tttgtgcgta cttactaaaa gtaagtttag gtgttagcgt atttaaacac aagacaaaac
     3541 gatggagact tcccatgatc gctattactg gtgccactgg ccaacttggt cagtatgtta
     3601 ttgaatcctt gatgaaaacg gttcctgcca gccaaatagt ggctatcgtt cgtaatccgg
     3661 caaaagccca ggcactgaca gcacaaggca ttaccgtgcg tcaggctgac tacggcgatg
     3721 aagccgcact gacatctgca cttcagggag tggaaaaact actgctgatc tcttccagcg
     3781 aagtgggtca acgtgccccg cagcatcgta atgttattaa tgccgcaaag acggctggcg
     3841 tgaaatttat cgcttatacc agcctgcttc acgcggataa atctccgctc ggcctcgccg
     3901 atgagcacat cgagacggag aaaatgctgg ctgattctgg catcgtttac accctactgc
     3961 gcaacggctg gtacaccgaa aactacctcg ccagcgcccc ggcagcactg gaacacggcg
     4021 tatttatcgg tgcggcgggc gatggcaaaa tcgcctcagc aacgcgggca gattatgcgg
     4081 cagctgcggc acgcgtgatt agcgaagccg gtcacgaagg caaggtttac gaactggcgg
     4141 gcgatagtgc ctggacgttg acacagttag cggcagagct gaccaaacag agcggcaaac
     4201 aggttaccta tcaaaatctg agcgaagccg atttcgccgc ggcactgaaa agcgtcggac
     4261 tgcccgacgg actggcggat atgctggcgg attctgacgt tggcgcatcg aaaggcggtc
     4321 tgtttgatga cagcaaaaca cttagcaaat tgattggcca cccaacgaca acgttagccg
     4381 aaagcgtaag ccatcttttt aatgttaata actagttaat taaagtggca tcctcccgca
     4441 tcctctctga taatgacggg atgccgggag caatcatgtc tgcttcctga actttcttct
     4501 gacagatcaa tggatgccag taatgattag cggcgtgctg tacgccctgt tagcagggtt
     4561 gatgtggggg cttatttttg tcgggccgtt gatcgtgccg gaatacccgg cgatgttgca
     4621 gtcgatgggg cgttatctgg cgttagggtt aattgcgctg cccattgcct ggctgggacg
     4681 cgtgcgtctg cgtcagttgg cgcgtcgcga ctggcttacc gccttgatgc tcactatgat
     4741 gggcaacctc atctattact tctgccttgc cagtgccatt caacgtactg gtgcgcctgt
     4801 ttccacgatg attatcggca ccctgccggt ggtgattccc gtcttcgcca atctgcttta
     4861 tagccagcgc gacggcaaac tcgcgtgggg aaaactcgcc ccggcactga tttgtattgg
     4921 catcggcctg gcgtgtgtga atattgctga gttaaaccac ggactcccca attttgactg
     4981 ggcacgttat acctctggca tcgtgctggc gttagtttcc gtggtctgct gggcgtggta
     5041 tgccctgcgc aacgcccgct ggctgcggga aaatcccgac aaacatccga tgatgtgggc
     5101 aacagcgcag gcgctggtca cactgccggt ttcacttatc ggctatctcg tcgcttgtta
     5161 ctggctgaat acgcaaacgc cggacttctc cctacccttt ggcccccgtc cgctggtgtt
     5221 tattagtctg atgattgcga tagccgtgct ttgctcatgg gttggcgcac tctgctggaa
     5281 cgtcgccagc cagcgattac cgacagtgat tctcgggccg ctgattgttt tcgaaacgct
     5341 ggcaggtttg ctgtacacct ttttactccg ccagcaaatg ccgccgctaa tgacgctgag
     5401 cggtatcgcg ctgttagtga ttggcgtggt catcgcggtt agagcaaaac cagaaaaacc
     5461 tttaactgaa tctgtctcag aaagttgaca cgctggcagt gagttaaata agcctctgct
     5521 acgtaagggt tatagctttt gccttaaaga tgcatttaaa atacatctta tcttattaag
     5581 aatgaggtat cagctatggc ttatcgcgac caacctttag gtgaactggc gctctctatt
     5641 cctcgcgctt cggctctgtt tcgtaaatat gatatggatt actgctgcgg cggtaagcaa
     5701 acgctggcgc gtgcggcggc acgtaaagaa ctggatgttg aggtcattga agctgagctg
     5761 gcaaagcttg ctgaacaacc gattgagaaa gactggcgta gcgccccgct ggcagaaatc
     5821 atcgaccata tcatcgtgcg ctaccacgct cgtcaccgcg agcaactgcc ggagctgatt
     5881 ctgcaagcga ctaaagtcga gcgcgttcac gccgacaaac cgagcgtgcc aaaagggctg
     5941 acaaaatacc tgaccatgct gcatgaagag ctttccagcc acatgatgaa agaagagcag
     6001 atcctcttcc cgatgatcaa acaaggcatg ggcagccagg caatggggcc aatcagcgta
     6061 atggaaagcg agcacgatga agcgggtgaa ctgctggaag tgatcaaaca caataccaat
     6121 aacgtcacac cgccgccgga agcctgcacc acctggaaag cgatgtataa cggcattaat
     6181 gaactgattg atgacctgat ggatcacatc agcctggaaa acaatgtact gttcccaagg
     6241 gcgctggcgg gtgagtgatc taaaagctgg atggcattgc gccatccagc atgataatgc
     6301 ggttatttcc gcagttcagc agcccgcttc ttaccaataa acaaccagcc cagccccagc
     6361 gcgataaacc acagcggggt aaccagcagc gcctggcgag tgtcatcttc cagtgtcagc
     6421 aacacgacca cgaacacaaa gaacgccata catacccagc acatcagctt gccgagcggc
     6481 atcttgtaga tcgacttctc atgtagatga ggacgctgtt tgcgatacac aaggtacgag
     6541 caaaggataa tcgtccagac gaacataaac agaatcgcgg aaacggttgt aatcatcgtg
     6601 aacgcgccaa tcacgctagg attcacatac aacatcacca cgccgccgag cagacagata
     6661 cacgagaaag tcaacccttt cgcgggtact gcgcgcttag aaagtttagc gaacgctttc
     6721 ggtgccacac cttcctgcgc cagaccaaac agcatacggc tggtagagaa gacgccactg
     6781 ttagcggaag acgccgcaga ggtcagcacc acaaagttga tcacgctggc agccgcaggc
     6841 aaacctacca acacaaacag ttcaacaaac gggcttttct ccgggactac cgaactccac
     6901 ggcgtcacgg acataataac aatcagcgcg aagacgtaga acataatgat acggatcgga
     6961 atggagttaa tcgcgcgtgg cagtgatttc tctggatctt tggtttccgc agctgttgta
     7021 cctaccagct caatccccac gaaagcgaaa accgctatct ggaatccggc aaagaagcca
     7081 cttaaacctt tcgggaacca gccgccgtca ttccacaaat gcgcgaatga cgcttccaca
     7141 ccagtcggtg actgaaagtg catcgccacc atgaccaggc cgacgacaat cagcgacaca
     7201 atggcgacga ttttgatcat cgcaaaccag aactccatct caccgaacat tttcacagtg
     7261 gcgaggttaa gcgtcagcag cagcactatc accgccagcg aggcgaccca gtcggagaga
     7321 tcggggaacc agaactgagc ataagccgtg atcgccacca cgtctgccat accggttaca
     7381 acccagcaga accagtaagt ccagccggtg aaatatcctg cccacggccc gagtaaatcg
     7441 gaagcgaagt cactaaaaga tttgtattcc agattcgaaa gcagcaattc ccccattgcc
     7501 cgcatcacga aaaagagcat aaaaccaatg atcatataaa cgaaaatgat cgacggcccg
     7561 gcaaggctaa tcgttttgcc agaccccata aacaaccccg taccaatggc accaccaatg
     7621 gcaataagct gaatatgtcg gtttgtgaga ttgcgccgta gcgactgttc agccggagcc
     7681 tgatcatcgg caacgacttt tacctgatct accatgtttt tttcttcctg tacctgtctg
     7741 tgttgttcag gctctacggc ctttagtcag tctatgatac gacgaatctg tatcgggact
     7801 catcgatatt aggtaacaat acgcgggatg aatactaaga tttagatcta atgttaattt
     7861 tatgtttaaa gtgagtgtca tatcacggtg ataacgcgaa acagctcaca aaaatacacg
     7921 cttacggggt ctttgcaaca caaaatagcg ccgtttggca aactgaaggg tttattgctg
     7981 aatgcctgct cccctctggt tcgaggggaa cagaatgcgc tgcttagagg atttccagca
     8041 gttcgacttc aaacaccagg gtgctgaacg gagggatgga tgcgcctgcg ccgcgctcgc
     8101 catatgccag ttcctgcggg atagtcagtt cccatttaga acctaccggc atcagagtca
     8161 gtgcttcaat ccagccaggg atcacgccat taaccggaaa ttcagcgggt tcaccacgag
     8221 caacggagct gtcaaacacg gtgccgtcga tcagtttacc ggtgtaatga acacgaacgc
     8281 ggtcggtacg tgccggaatt gcgccttcac cctggttgat cacgcggaat tgcaggccag
     8341 attcggtgct attcacacct tcttttttgg cgttttcttc caggtatttc acaccttcag
     8401 cagccatcgc ctggaaacgc tgacgacgaa cggcatcggc gcgctcgtgg atttcacgca
     8461 gcgcgcgatg caccacatca accggaacag ccggatgttt gccttccagc gcatcggcaa
     8521 tacctgcaac cagtgcttct ggcagcagcc cttccaggcc agattcactc agttgttgcc
     8581 ctacctgcaa accaatgccg tagcttgctt gcgcttcgat ggtgtcaaaa gttggggtgg
     8641 tcatcgtatt tcctttctta gggtataaag tagcgggcag catatcagcc acacttcttt
     8701 gggtaaaact ttgtcgcggg aatgatgaca aatcccaggg aaaaagaaac aatagcaata
     8761 tcccgtatga atataataaa ggcgggttta ccacggacat catagcgtta gcttattcat
     8821 aggctatgat tgaggaacaa gacgcggagc aggaggaaac catgcccggg cgctttgaac
     8881 taaaaccaac cctggagaaa gtctggcacg caccggacaa ttttcgcttc atggacccgc
     8941 tgccgccgat gcatcgtcgg gggatcatta ttgccgccat tgtattggtg gtcggatttc
     9001 tgcttccatc tgatgatacg cccaacgcgc cggtggtgac gcgtgaagcg cagttagata
     9061 ttcagtcgca atcacaaccg ccgacagaag aacagctgcg ggcgcaatta gtcaccccac
     9121 aaaattatcc tgatcaggtg gctccggtcg ctccagaacc tatacaggaa ggccagccag
     9181 aagaacagcc gaaaactacc caaacgcaac cattccagcc agatagcgga attgataatc
     9241 aatggcgctc ttaccgtgtc gaacccggta aaacgatggc gcagctgttc cgcgaccacg
     9301 gactgcccgc taccgatgtc tatgcaatgg cgcaggtaga aggcgcgggt aagccgctga
     9361 gtaatttgca aaacgggcag atggtgaaaa tccgccagaa cgccagcggc gtggtgacgg
     9421 gcttaaccat tgatacgggg aataatcaac aggtcctgtt cacccgccag ccggatggca
     9481 gttttattcg tgcgcggtag atgaagggtc aaccaaaagc cagctaagga ttgtctcttt
     9541 gatgacctta cgtctggctt ccagtgcctg gggtgaaacc atactgatgt gttgacatcc
     9601 tccaagccct gaaggacgtg gatccctgct acgttcaggc tgtcgcctga atcatttcgg
     9661 tgggttcctg cttcaacggg cggcctgact gcaccatctc cccacaggca agaacggcgt
     9721 gccccgccgc taaaatgtta cgagcgccat ttacatcagc gttcgctgta tatccacatg
     9781 cctggcatct gaatttactt tgtgacaggc gattttcttt cgctgtatga ccacagcacg
     9841 cgcaacgctg gcttgtgtac gctggcggaa cagcaagcac ctgaccgcca cgccagagct
     9901 gcttgtactc aagctggcgg cgcatttcat accagccctg atccagtatc gaacggttta
     9961 aacctgattt tgcccggaca ttgcgccccg gctggctgac cgtacccgct gctgactttg
    10021 acatgttgct gaccttcaaa tcctcaatga ctatcattgc gtggtttttg ctgacggtcg
    10081 ttgtgacttt gtgaaggtag tccctgcgga tatttgcgat acaggaatgc agtcgctgta
    10141 ttttgcgttt ctgcttctgc cagttgttgc tgaatttgac cttgcggctt aactgtcgct
    10201 gaagtctcgc cagcgtcttc tggtttttct gaaaactgtt tacaggtcca aagactgtgc
    10261 catctgacag cgtggcgagt ttagccacgc cagcatccag tccgaccatt aatgctgaag
    10321 ggtgaaccgg agttgatact tcattttcag tctgaatact gatgtaccac ttaccgcagg
    10381 actggctggc agtgacattt ttcacaacac ccgtgacctg acggctattc cggtagcgca
    10441 tccagccaag tttcggcaga aaaatacggc tgttttcctg atcgagctta acgccctgcg
    10501 ggtagcggaa tgcatcattc tgtccccgct ttttgaatcg gggaaaagca gcccgcttcc
    10561 ggaagaagtt tttgtaagcc cgctcaaggt ctttcagtga ctgttgcaat ggctgtgacg
    10621 gggcatcttt aagccattgc gtttcagtgg catttttcca ctcaaccagc caggaagcca
    10681 ttttcccgta agggatgtat ttattaccgg cctcatgatt ctcattctga agcgccagag
    10741 cacgattgaa aacgaaacga catgcgcctg cgaagcgcct catttcacgc tcctgttgac
    10801 caccgggtct taactggaat ttaaatgcct gtagtcgttt cacaggccaa atgatacttt
    10861 ggtctgtgaa aaaagaaacc gatatccggc gtggaaggca ttgtgttttc ctgatgcatg
    10921 ttcacctggt ctttgtcacc agataccgac gccagatttt tgaccatgac gcgacagaaa
    10981 aactacgcac ttacttttca aaggtatgtg ctgattttga agctgaactg gttgaaatgg
    11041 atggcgaacc agatcacgtc catttgttaa ttaactatcc tcccaaactg gcgatatcca
    11101 gtctggtaaa cagcctcaaa ggcgtatcgg gtaggttact gcgacgagat cgaccagata
    11161 ttgcagtcag gtattactac aaaggcgttt tgtggagtcc tggctatttt gccagtagct
    11221 gcggaggtgc gccaatatcc gccatccgtc aatacattga acaacagcaa acacctggtt
    11281 aggtggaaaa ccgcgccgta tatccccgcc ctgaaggacg gggttttacg gcgcaccgga
    11341 taaaccaggc caagcgtatg ctggttgatc aaataatatc ccccaagcgc agcaatatta
    11401 atattcacct gcaatgggtc gatatctggc ttaaaaatat tgtatttctt cccttcatcc
    11461 aataaggatt ccattaactg caagtgcgca tggttgattt ctaagagacg ctgtgatttt
    11521 gcataatgca cacctttact ttgattttca ctatgcacaa tttttaaaaa ccaggggtta
    11581 gcaatataat aatcccatgt aaaatcaata attttctcaa tggctttttg tggctggtta
    11641 acctgctgat taacgaatac accgataccg acagcgtaga ataattacat accgcacaac
    11701 aagaatgtac ggtgtaaggc gtggaagtca ttttctcccc gggtgcatcg ttatatccat
    11761 aatgagcgat ttttgatatt aatatctttc accatccgct attttcacca cttcagaaaa
    11821 caaacttatt tttattaaaa tcgatttttt tctgtgaaca ttcagctgcg atatttataa
    11881 aaacattaaa caggtcacat tacaatgaca ttaccaacca ctatttattc atttcctgca
    11941 tatttatctc gtttttcgtc caccgacaaa ccagtaaaat taaaatttca tcaatacgca
    12001 cgcgctacat tactttcaaa tcgtggaaga gatcataatt gcgatggcag acgtactgtt
    12061 gagatccaca aactagatct ctcagactgg caagcattca ataagttagc aacgaagtgt
    12121 aatgcttacg atggagtaac aatgaatggt gataattctt ttggatggaa ccatgaagct
    12181 acattagata atattcacgc ccagaaatac aataaagctt acgcgggtgc gaggttaacc
    12241 gcagaactga aatatttatt gcaagatgtc gaatccttcg aacccaacag taaatacaca
    12301 atacacgaag tggttttggg acctggttat ggtactccag actataccgg ccaaacgata
    12361 ggatacgttg taacccttcc tgcccaaatg cctaattgct ggtcatctga actaccaaca
    12421 atcgatcttt atatcgacca acttcgcact gttacaggcg tttctaatgc cctcggattc
    12481 atcattgctg cattgctaaa cgcgtattct gatttacccc atgatttgaa aattggtctg
    12541 cgttccttat caagttcagc cgcaatctac tcgggtttgg gtttcgagcg agtgccgcag
    12601 gagcgagaca tcagttgcgc tcgtatgtat ctaacgccag caaaccatcc tgacttatgg
    12661 acacaagaga atggtgaatg gatctacttg cgaaactaat acctgacgaa aaatagaaag
    12721 caaaacgccg accagtggtc ggcgttttta cgtctcgttg aataacgaat tattcagcta
    12781 ctacgtttac gatcactttc gcgaatactt cgctgtgaac ctggaagctc acttcgtgtt
    12841 cgccagtggt acgcagaacg ccgttcggca gacgaacttc gctcttagcc acttcaacgc
    12901 cagctgcagt tacagcgtca gcgatgtcgc gagtaccgat ggaaccgaac agtttacctt
    12961 cgtcgccagc tttagacgcg atggtaacag tttccagtgc attgattttc tcagcgcgag
    13021 cattagcagc tgccagaact tcagccagtt tagcttccag ttcagcgcga cgtgcttcga
    13081 agaattcaat gtttttcttg gtagctggaa cagctttacc ctgtggtacc aggaagttac
    13141 gagcatagcc cgctttaacg tttacctgat cacccaggct acccaggttt gctactttat
    13201 caagcagaat aacttgcatt accttatcct ctcaaagtcg tattaatgga ccgtgaccga
    13261 ttactgatgg cgatcagtgt acggcagcag ggacaggtag cgagcgcgtt tgatagcgcg
    13321 agccagctga cgctggtatt ttgcacgggt accggtgata cggcttggga caatcttacc
    13381 gctttcggtg atgtagtttt tcagcgtagc gatatcttta tagtcgatct cttgaacgcc
    13441 ttccgcggtg aaacggcaga acttgcgacg acggaaataa cgtgccatat ggctagtctc
    13501 cagaatctat caattcaatc tgctcggcat gcaaaaccat tttgctcagt ccgttctttg
    13561 ccttgtggca tgaaatgaac ccctgaacgg ttatgcgact gccgaccgtt atactgtgag
    13621 taatggcctg gttttcgtgt ccgctaacaa taacgggcat ttgacaccat gcctgccggt
    13681 gaaagccggc ttcctcctgc acagaacgat gctcaagcac gaactggcag tgaggaattc
    13741 ctgatggact gacctttcga aggggagtcc tgcacacggt gccggacaac accagacggt
    13801 tggtcatcag aaattactct tcagaatccc cagcttcagc atcatcagcg gtttcgtttg
    13861 cgaaatcatc gcgacgctca cggcgctcgt ctttcgcttt aaccatcgga gatgcttcgg
    13921 taacagcgtg cttggtacgc ataaccatgc tgcggataac ggcatcgttg aagcggaagg
    13981 tagtttccag ctcatcgatc acttcctgcg gagcttcaac gttcatcaga acgtagtgag
    14041 ctttgtgcag tttgttgatc gggtaagcca gctgacggcg gccccagtct tccagacggt
    14101 ggatcttgcc ttctgcacca gtgatggcag cagtgtagcg ctcgatcatg cccggaacct
    14161 gttcgctctg gtcaggatgg accataaaaa cgatttcgta atgacgcatc gaattgctcc
    14221 ttacggatta ttcagcctcc tgtctgggtc agccgaatcc cggggaggca aggaacgtgt
    14281 taaaggtcgg ctgaaaaatg acgcgttata gtactgacat accccgctaa actcaaggcc
    14341 agccgacaaa taaatctcat ctttcaccaa agtggactaa cacgctgatt tgaagttcat
    14401 cacacttcat catggtgttt tttcgaacaa ctgcatcaga aatggtcacc atcaacttct
    14461 ttatcattga ttaaaattaa atcagaagat catcacagag gagagcacac aaagtcagcc
    14521 gtcgaggagg tatctatgtt cagtcgagtt ttagcccttc tggctgtgct tttgctaagt
    14581 gcaaatacat gggcagccat tgaaattaat aaccaccagg ccagaaatat ggacgatgtg
    14641 caaagcttag gcgtgattta tatcaatcat aatttcgcca ctgaaagcga agcacgtcag
    14701 gcattaaatg aagaaacaga tgcgcaaggc gcaacgtact accacgtaat tctgatgcgg
    14761 gaaccgggga gtaacggcaa tatgcacgcc agcgcggata tttatcgcta gcaccaggta
    14821 taaccaacga aacattgcca tagtttgctt tgcccccttc gcagggggct ttttttgaaa
    14881 aacggctgcg gaattagacc agttatctcc cgaggaagga aatttccgca gcgcgtgttc
    14941 tacttctgcc cgtaataagc gttggggccg tgcttacgca tgaagtgttt attcatcagg
    15001 aagctgtcga tgtgattgag ttgtggatta atactgcgcg caatccacgc cattttcgcc
    15061 acttcttcca tcactaccgc gttatgcacc gcgtcgtggg catctttccc ccaggcgaac
    15121 ggtccgtgct gatacaccac aattcccggc gtatgcagcg gctcggcgtt gcccagcgtt
    15181 tcgataatca ctttgccagt gttcagttca tactcaccct gcacctcttc ttcgcttaat
    15241 ccacgcgtac acgggatatc accaaagaag tagtcagcgt gcgtggtgcc taacgccggg
    15301 atcgccagcc ccgcctgcgc ccatgcggta gcatgagtgg agtgggtatg gacaatgccg
    15361 ccaagcgacg gataacgacg gtagagttcg agatgcgtcg cggtgtcgga agacggacga
    15421 tattcccctt ccaccacctt accgctcata tcgaccacca ccatatcgtc tgctttcatg
    15481 gtttcgtagg cgacgccgct gggcttgatc accaccagcc cgcgttcgcg gtcgatagcg
    15541 ctgacgttgc cccaggtaaa ggtcaccagc ccatagcgcg gcagatccat gttggcttca
    15601 aacacctgct gttttagctt ttgcattatg ccgcctccac catgcccgct ttcgccatgc
    15661 gcgctttcac ccaatcacgc gctttcgcca cttctgccgc cgggtcttcc gccgtttcgc
    15721 tccacatctc aatcaggtac ggcccgcaat agccactctg tttgagcgtt tcgaagcaac
    15781 gttcgaaatc caccacacct tcgccaaatg gcacgttttt gaagacgccc ggtttggtgt
    15841 ctttcacatg taccgcgacg atatgcccga ttccggcctg caactccatc tgcacatcgt
    15901 tgtcccacgc cgacaggttg ccgatatccg ggtagagctg gaaccacgga ttgttgagat
    15961 agtgcgcgta gcccagcgcc ttgctgatgg agttcatcaa cggataatcc atgatctcca
    16021 tcgccagcgt cacctgcgcg cggctcgcca tctcaacgct ctctttcagg ccgtcacgga
    16081 aacgacgacg cgtttcgtta ttggcttcct gatagtaaac gtcatagccc gccagttgga
    16141 tcacgcgaat accaacgtcc tgggcgaact ggatagcttt acgcataatc tccagcccct
    16201 gcgcccgcac cgcgtcatct tcactgccca gcgggaaacg acgatgagca gaaaggcaca
    16261 tggacggcac gcgcacgccg gtttcaacaa tcgcattcac cagcgccaga cgctgctcgc
    16321 ggctccagtc gaggcgcgac aggcgctcgt ccgtttcatc taccgacatc tcgacaaaat
    16381 cgaagcccaa cgtttttgcc agttgcaggc gttccagcca gcactccccg gcggggagcg
    16441 ctttttcata gatgccaagc gggatttgtt tggacaacat atccgctcct tagccccaca
    16501 gttcggcgat ggaacgtttg aactgacgtg cggcttccac cggagaagcg gcatcacgga
    16561 tactacggcc cgcgataaag acgtgaatcg gaatgccttt gaacagcggc agatcttcca
    16621 gcgccaggcc accagtgacg gtgactttga agcccatatc ggaaagacgt ttgatcgcgg
    16681 tgatgtccgc ttcgccccac gccacgcctg cggcctgcgc gtcacggctg cggtgataaa
    16741 ccacctgctg aatacctgcg tcgcgccact gttgcgcctg ttcccaggtc cagtaaccgg
    16801 tcagttcgat ctgcacgtcg ccgttaaact cttttgccac gtccagcgcg cctttggcgg
    16861 tgttgatatc cgcacagcaa attaccgtca cccagtcagc gttggcttcg aagcacatac
    16921 gcgaaaggat tttgcctgca tcggcaattt tggcgtctgc cagtacgatt ttgtgcgggt
    16981 agagcgcttt caggtcacga accgcacgca cgccttcgcc cacgcacaga atggtgccca
    17041 cttcgataat gtcgacttct tcggcaatca ggcgagtggt ttcgtaggcg ctatccatag
    17101 tctggttgtc cagcgcgact tgcaacatcg gtaatgacat gttcaattcc ttcttaagct
    17161 gccgcgttgg tgcggtcaat gagatccagt acttcctgct cggtacggca ggcgcgtaaa
    17221 cggtcaaaat tctcttcatc ttcaaacagg ttgacgatct gcatgatgcc cacttcctgg
    17281 tgagtattgg catcgaccgc cgccatggtg atgaggatat ccaccggatc gttgtcatcg
    17341 tggttgaact ccagcggctt tttcagcgtt accagagaga aaccggtttt cttaacgccc
    17401 tcttccggac gcccgtgcgg cattgccagg cccggagcga taacgaaata aggaccgaac
    17461 tgttcaacgc catccagaat cgcctggtag taacgcggct ctaccacatc tgccgccacc
    17521 agcaggtcaa cgccgatttt caccgcttcc tgccatgtct ctgcttcagc ctgcagacgg
    17581 atggatttat tttcagccag cgaatcacgt aatttcatgg cgcgtcctta cttcacgtcc
    17641 tgcgggaaat gctctttgat cacttccagc agtttcgggc caaagtcggc aggagagagc
    17701 atgttgcgca cgccaaccac gtatttgttg ccggtcacgg tgatttcgcc cgcaatgtgc
    17761 gtagaagcga tgatgatatc cgcgccactc aactcgcttt tgtactcgcc aaccgcgcag
    17821 ctgtttaccg tatggtcaat gtttgattgg gttaaaaact ggtccacttt catcttcatg
    17881 atcatggaac tgccttgtcc gttgccacac acagccagaa tacgtacggt cataatcaaa
    17941 actccttatt aagcagactg ttctgccagt tgtttttctg catcttcttc tgcgcgcagc
    18001 gcgcggccag cgaagaacat ataagccagt gcaattacaa tgatgacggc cataaaggcg
    18061 ataccgatgg agaagaagcc ttgcatcatc ggcggtgcca gaatcgacca gtccgccatg
    18121 cccatccagg cactcatacc ggtgagtttc accgcccaga cgcaaccaaa gatttcgatc
    18181 attcccatca ccagacaaat cttcagcgct gcacgccagc cgccgaagtg gttagcgaac
    18241 acgccgatgg tggcgttgga gaagaacatc gggataaagc ctagaataat cagaatggaa
    18301 gagccgcagg cgaccaggat gccaactgca atcagctgac cgatggtgcc ccacataaag
    18361 ccccagacca cggcgttcgg cgcgaagcta tagatagccg cacagtcaat cgccagaacc
    18421 gcacctggga tcaggcgctg ggagataccg ttaaatgctt cagagagttc cgccacaaac
    18481 atgcgcacgc cctgagtgat gatgaagatc gccaccgcga aggagaaacc ggtttgcagg
    18541 atgtagaccg tccagtgcac tttgcccgcc atcgcctgca cggtgtcgat accgaaggag
    18601 agcagaatgg cgccaaagaa gatggtcatc acaatcgccg tggagacgat gttgtcgtgg
    18661 aaaatgttca gccagcccgg cagtttgagg tcttcaacgc tctcctcttt tttgccgagg
    18721 aacggcgcga ctttataggc aatccatgat gcaaactgct gctggtgacc gatggagaaa
    18781 ccacagccat ccgtcacttc ctgagtcggc ttgtacatca tgttggaagt gatgccccag
    18841 tagagcgaaa ccagaatcgc ggtacagata atggtggtcc acatggagta gccgaagata
    18901 aacagcgtaa cggcaatcaa cccggcctgc tggaacatga tgtggccggt caacatgatt
    18961 gtgcgaatgc cggtaatgcg acgcaacagc acgtaacaga tgttcagtgc cagcgctaac
    19021 aatacggcgt aacccaccca gctataggca tcgcccatgc ggtcgatggt tgccatcatt
    19081 gaagcgtagg tatcggaaat tgcgccgtta atgccgtaga cttcggacat tttcgccacc
    19141 accggtttga aggtgctggt gaggatgccg gaccctgcct gcaacaacat gaaaccaatt
    19201 atggttttaa tcgtgccttt aataataacg ctgacacttt tgcgcagtag gatgtagccc
    19261 agacaggtca caatacccag caacaacggg gcattggtca tgacctggtt aaaaaatacg
    19321 gtaaagatgt tgtagaggat ctccataacg atctcctgaa gaagaggtaa ccgggtattc
    19381 cacacacccg gaatgttgtg cactcactct aattttcaaa agtaatcaca acaagattat
    19441 ttatgattaa atgtgacgcg acccgcaaat aattccacat ggatagagcg ggattcacgc
    19501 aacgaaacaa ttattcattt aaattcattt agttaattga attatacatt ccattaaatc
    19561 tttccatact caaaatcaac aacaacttcc ctggcaaaaa gtcaaaaaat ggtcattgcc
    19621 ttccagaaaa aatagtgtca ggattaatca aaaccaatca catattgatt cgaattgaac
    19681 tatcatgaag gtaaatggcg atgagtaaag tgaaaagtat cacccgtgaa tcctggatcc
    19741 tgagcacttt cccggagtgg ggtagctggt tgaatgaaga aattgaacaa gaacaggtcg
    19801 ctcctggcac atttgcgatg tggtggcttg gctgcaccgg gatctggttg aaatcggaag
    19861 gtggcaccaa cgtttgcgtt gatttctggt gcggcactgg caaacaaagt cacggtaacc
    19921 cgttaatgaa acagggtcac cagatgcagc gcatggctgg cgtgaaaaaa ctgcagccaa
    19981 acctgcgtac caccccgttt gttcttgatc cgtttgcgat tcgccagatc gacgcggtac
    20041 tggcgactca cgatcacaac gatcatatcg acgttaacgt cgctgctgcc gtgatgcaga
    20101 actgtgctga tgacgtaccg tttatcggac cgaaaacctg tgtggatttg tggattggct
    20161 ggggcgtacc gaaagagcgt tgcatcgtgg tcaaaccggg cgatgtagta aaagtgaaag
    20221 acattgaaat tcatgcgctt gatgctttcg accgtactgc actgatcacc ctgcctgccg
    20281 atcaaaaagc ggctggcgta ctgccagatg gcatggacga tcgcgcggtg aactacctgt
    20341 tcaaaacgcc tggcggctcc ctgtatcaca gcggcgactc ccactactct aactattatg
    20401 cgaagcacgg taacgaacat cagatcgacg tggcgttagg atcgtacggc gaaaacccgc
    20461 gcggtatcac cgacaagatg accagcgccg atatgctgcg tatgggtgaa gcgctgaatg
    20521 cgaaagtggt tatcccgttc caccacgata tctggtcaaa cttccaggcc gatccgcaag
    20581 agatccgcgt gctgtgggag atgaaaaaag atcgtctgaa gtatggcttc aagccgttta
    20641 tctggcaggt gggcggcaaa tttacctagc cgctggataa agacaacttc gagtaccact
    20701 atccgcgcgg tttcgatgat tgcttcacta ttgaaccgga tctgccgttc aagtcattcc
    20761 tgtaatctgt cgtttatacc ggatagcgtg gagaagttcg cgctatccgg cgtagtattt
    20821 cctggctatt tcaaatatca tctaaaaaaa tcaaatttta tcggaatagc tcatgactga
    20881 agcacaaaga catcaaatcc tcctggaaat gctcgcacaa ttgggctttg tgaccgttga
    20941 gaaagtcgtt gagcgtctgg gaatttcgcc tgccactgcg cgacgcgata tcaataaact
    21001 tgacgaaagc ggcaaactga aaaaagtgcg caatggtgca gaagctatta cccaacagcg
    21061 cccgcgctgg acgccgatga atctgcatca ggcgcagaat cacgatgaaa aagtacgtat
    21121 cgctaaagcg gcctcgcagc tggttaatcc gggcgaaagc gtagtcatca actgcggctc
    21181 caccgcgttt ctgcttgggc gggaaatgtg tggcaagcca gtgcaaatca tcactaatta
    21241 tctaccgctg gcaaattacc tgatcgatca agaacatgac agcgtgatca ttatgggcgg
    21301 acagtacaac aaaagtcagt ccatcacttt aagcccgcag ggcagcgaaa acagtctcta
    21361 tgccgggcac tggatgttta ccagcggaaa agggctgacc gcagaagggt tgtataaaac
    21421 cgatatgctg acagcaatgg cagagcagaa gatgctgagc gtggtaggga aactggtggt
    21481 actggttgat agcagtaaga ttggcgaacg cgcgggaatg ctttttagcc gtgccgatca
    21541 aatcgatatg cttatcaccg gcaaaaatgc taacccggaa atcctgcaac aactggaagc
    21601 gcaaggggtc agcattctgc gtgtttaaag atgctggcgg aaaaatgtca cggcagcatc
    21661 taacgcctca ggcgtaatgc ggtgacgcac gcctggctgc catgaacagg ttagcagttt
    21721 atcccgtccc gtctcgctta aggcctgctg caaacgtagt gattcgtcgg caggcacaac
    21781 gtcatcgtcg aggccatacc acagcagtag aggtctgtca ctaagttgtt ccaggtggtt
    21841 tgtcgcttcc cactctgcca gtggcgcgac aatgttattg aattcattct gctgtgctac
    21901 cgtttcaggt atcagcggtg gaaacagtaa acgggcgagt gatgtaaaat agcccgatcc
    21961 catcatgctg gcggtacatc tcaccgtggg gtggcgagcg gtaatcccca gtgccgtcat
    22021 cgcgcccatc gacgcaccac cgactgccag acggtcatca agcagccact tttcttcggc
    22081 tattgccgca cgtaaagtag tgaattcctg catactttgt agcaagattt gccagaattg
    22141 atttaaccgc cgcgctgcgt caccactaaa acggctaccg tgatcgggcg catccggcat
    22201 gatcacccgc aaaccagcct gcgccagcgc aacggcaaaa tagctataca ccagactgga
    22261 cgaagtaaag ccgtgataaa aaatcacgca cggtaacggg gtatcttttt gtccgacagg
    22321 ataagcatga agaacgggaa tatctgccag ctcgcgtgat tctatttcaa tcatctgtct
    22381 ctcctttttg tctggtatga catgaagaaa tccttagcac aagtttctgc tggcggtatt
    22441 agttcataat gttgagatgt gggttacgct ttcgtcacgt tttcattcga aaatcgcgta
    22501 ccaggtaaca attcgggaac attcccccaa agttaaatta ataaggcact acactatggt
    22561 tagcaggaaa cgtaatagcg ttatttaccg gtttgccagt ttattattgg tgttgatgtt
    22621 aagtgcctgt agcgcactgc aaggtacgcc acagccagca ccgccagtaa cggaccatcc
    22681 gcaagagatt cgccgcgacc agacgcaagg gttacagcga ataggtagcg taagcacaat
    22741 ggttcggggt tctccggatg acgcattagc agaaatcaga gcaaaagctg tcgctgcaaa
    22801 agctgattat tacgttgtcg tcatggtgga cgaaaccatc gtgacaggac agtggtattc
    22861 acaagccatt ttgtaccgta aataaatcag acaacttaca tagtcagctt tacattgctt
    22921 tgcgtcccta tgcgtttacc tgtgcacaat aaattaccgg ctggaagcct gggcgtttat
    22981 taccgcgatg gaatgccctg caacatgtgg ggaaacgaaa aatggagctg acgatgaaac
    23041 aattacttgc ctcaccctcg ctgcaattag tgacttatcc tgcgagcgcc acggcacagt
    23101 ctgcagaatt cgccagtgct gactgcgtga cgggcctgaa tgaaataggt cagatctcag
    23161 ttagcaatat ttcaggggat ccgcaggacg tggaacgtat cgtggcgtta aaggccgatg
    23221 aacaaggtgc gtcatggtac cgcattatta ccatgtacga agaccagcag cctgataact
    23281 ggcgcgtaca ggcgatactg tatgcatgag tgtcatctac agcaaatgaa caatgccgga
    23341 tgcggcgcga gcgccttgtc cggcctacaa ttttcacata tttcatttag ttaatcgaaa
    23401 ccagcgtcgc atcagtcgta tacgcttaca cacacactcc ccccgtcgcc cgcagcaata
    23461 aatcattctg gatctgctct gacaaccgta cgccgccgcg cgtatctaac atcacctgac
    23521 accacgcctg cgccattggc ggagaagcat atttcaacat ttgcgcaccg cagcccagca
    23581 ggaatagctg atgagtaatc tctcgcccca gttcttcagc tggcttacgc agctgctgct
    23641 gtaaacgacg aaccgcgcaa tcaaaatagc gatcctgccc tttcacttct acaaatgctt
    23701 ccgacaataa gtcgtatacg cccgcttgct tattgagaac gcgcagcaca tccaggcaca
    23761 taatattgcc ggaaccttcc caaatactgt ttaccggcat ctcccggtaa agccgcggca
    23821 gctcgctctc ctcgcaataa ccaatgccgc ccagcacctc catcgcttcg gccacaaacg
    23881 gcatacctcg tttgcagatc acaaattttg ccgcaggcgt aaataaacgc gcccacagag
    23941 cttctttggc atcggcgcgc cggtcccacg ctcgcgcaag acgaaacagc aacgccgttt
    24001 gcccttcaag ctgaagtgcc atgcgactta agacatgacg cataaggggc tgttggatca
    24061 atggattacc aaaaacatgg cgttgatgcg catgataaat cgccagcgag aacgcacggc
    24121 gcatcatggc atggctaccc agggcgcaat caaaacgcgt catccccccc attttcagga
    24181 tcagacgaat tccttcccct tcctgcccca ataaccagcc aatggcatcc tgaaactcca
    24241 cttcacaact ggcgttagag cgattaccca gcttatcttt cagccgctcg aggcgaatcg
    24301 cgttgcgttg cccgtcaggc aaaaagcgcg gcacaaaaaa gcaggacaaa ccgcccgcgg
    24361 tctgcgccag caccagatgc gcatcgcttt gcggaactga gaaaaaccat ttatgcccca
    24421 ccagccgata agagccatcc tccagacgct ctgcgcgggt ggtgttgctc atgacatcgg
    24481 aaccgccctg cttttccgtc attcccatgc caatcaacaa accgcgtttt tgcccacctg
    24541 gcaataagtg agaatcgtag cgatcgctca acagcggtgt ggtccagtcc tgaaacggcg
    24601 cgggtaacat ctgtaacaac aatggcgtgg cggcaaaggt catggttatc ggacataacg
    24661 accccgcctc aacctgtgcg tgcaacataa aacgcgccgc gcgcgccaca aatgcgccgg
    24721 agcgagcgtc ttcttcccag gcaagattgt gcacccgatt ggtacatagt gcctgcatca
    24781 gcaggtgcca ggcggggtga aaacgcacat cgtccagacg gcgtccttgc gcatcgtagc
    24841 gcaatagttc aggcggattc acattcgcca gccgccccag ttcaagggat tcagccgttc
    24901 ctaactgctg accaatactg gctagaaaat cgctatccca gccagcacct tcacgcgtta
    24961 ccgcttcgca aagcgcgcca tcagacaggt ataagttgct gttatttaat ggtataggtt
    25021 gattaaaaac ggtgtgagtt tgccagtgca ctgtgtctcc ctccatcaat ggcagtcacc
    25081 attagtatgg tcactgccat ggattcatga cagaaagaga ttgctaaaac attcttatcc
    25141 agcaatgtaa tgcggaacga aacgtgaggt gtctttggtg atcagtgtgt tatcttcacg
    25201 gatccccatt ccgcacgctt catcatcgac aatccagcta ccgatgagtg tgtagctatc
    25261 gccaaaccgc ggcagaggtt gaaacgcctg gtagatcatc ggttcatcgg cgtaatcacc
    25321 atcagcgtgg tcaacgacgt tattctggcc gtcgaaaatg gtgacatttc cgccttcgcg
    25381 cgagtagatc ggtttacgca catagctttc gccagcggca atctgcggtt tttcaccctc
    25441 aaaccaggat gcaagaaggt taggatgacc agggaagaag cgccaaagca gcggcattag
    25501 ccctttatta ctcaagatac ttttccataa cggctccacc cattgctcgc gacgcttgcg
    25561 cagcagcgga ccgttatcgt cacgcatcat ccactccagc ggatacagct taaatgcacg
    25621 ctggatgaca ttatcatcaa gatcggtcag tacgccgccg acgcccaaac cgagatcttc
    25681 aatgtagata aaccgcgact cctgccctgc ctgctgggcg cagtcctgca agtacagcac
    25741 ggtactcctg tcttcatcgg tgtcctgaca gcagcaaaaa taaaacggtt cccgactgta
    25801 aagctcgcta aagcgtgaaa tcaggcgttc ctgaatagca ttgtactgat cggcatcacg
    25861 cggaataatg ccgctgcgcc gggcatcctc cagccacagc cactggaaat aagccgactc
    25921 gtacaatgaa gttggcgtat cggcgttgta ctccagcagc ttcaccggcg cattgccaca
    25981 ccaggcaaaa tccatccgtc catacagcga aggatcgcgg gcgcgccagc tttcagcgat
    26041 cacatcccag tacaacggcg ggatcgccag ttgcgtcagg atctcttcat cttttaccgc
    26101 gcgatccacc acctcaaggc acatctgatg cagttccgca gtcggttttt cgatctgctc
    26161 ttcaatctgg cgcagagtaa aacggtaagc ccgactctca tcccaataga tttcattgtc
    26221 gatgatatga aagtcgaaac cgttgtcagc ggcgatctgg tccagatccc gtcgcacagg
    26281 aacgttgtgt ctcagcatga ttagcctccc caatgcccac gggcgctgga agaacgacca
    26341 tagccgccgc gagaaacggt tgacgctttt ttggtggtaa agccgcctga agagtaagac
    26401 tcttttttgc cagaaccgga gcgccaggag taatcaccgg aagtgttgcg ccagacgggg
    26461 cgggaagcaa atgaggatcc gccgctgtta tagacaaacg gctcatcgcg atctttacga
    26521 ataacccggc ttaacaaaaa tccagaaacg accgggatcc aactctgttc aacattgtcg
    26581 tagtagcaat tttcgtactt agactgacag ttctgctgag tcatattctt cggaacatcg
    26641 gcataaaatg ccgctttggc gttgttccag ccacgcgcgc aaatgtcggc attattaccg
    26701 tcatcaatac aatcctgcac tgtcgaataa aacgttccgt cgccgtcgtt atcgacatcg
    26761 ccactatcgc cacaaccttt caatacaaaa aaagcggcac cgcccattag cgccaacgtt
    26821 aagtattttt gtgcgtagcg attgcgacgc ggttcaaaag gattattcgg tctaccaatg
    26881 cgactgatcg ctccgtgacc aattttactg ttatttcttg atttgcgttt tctggccatg
    26941 atcgtccatt accatgtcat acaagctgcg ttaaaaatac cgccagccag cgcggcggtt
    27001 cccataaaca ttcctgctgc ggtattgtga ttaataattt tttcgcttaa tgcgggcata
    27061 taaagtctga caccagcaaa aacgagtaac tgaatcacca gtgcaatccc gccccaggcg
    27121 aaatagtctg gaatactcac cgcattgatt gccgcactgg ataaggggat cacgtaaccc
    27181 aacaatgtac cgctgaacgc cagtgacgct gcggtattat tgtttttgat taattgccat
    27241 tcattgtgcg gtgtaatttt ggagtagata aacagaaaaa taatcaccat agccacgcca
    27301 ataaaaaaat aggcactaaa ggcaagaagt gaatccagta tatgcattgt aaacgtcctt
    27361 gtaattaacc aataatatga agtgatgtca gcgggatatc gacacccaac gcgcgcgaaa
    27421 ataaccactc tggctcgccg agatcgttaa aagattcctc accatttagc agcaaatatt
    27481 cgtaagtatc ttcggtcact tgccgctggt agcccatggt aaaattatgg acttcccatt
    27541 tagcatgatt ttgattttct actttttcca gcatgtagac gggttcgata tttcccggtt
    27601 cttcgctgtt aaaaaatcgc tgccagcgtt tttcctgcca gtttaaggtc attgccccca
    27661 tcgttttggc gttgatcgcc tcgcgccagt gattctcttt actgataccg tagctctctt
    27721 catagacaaa gagcttgata tcatcaatgt catcaatatc ttcgccgccg gtggtattga
    27781 tttgcagaaa ctcatcgcct gaagtgtagt agcggaaaat ctgactaccg ccgcccagat
    27841 cgatgtggct gacagcgact accgtaaatt cctcacccgg cagcgcaatc agcaattcat
    27901 cttccagcaa acgaaacgct aacgtgtcga gcgtaaagcc actattgaga tgaagtccca
    27961 gcggaccgcg agcgatagca ggcttattat ccttgccaaa cagacgctgg aaaaaaccag
    28021 acatatttta tccttaaaat agttgccagc cttgcagtga ggctggcaaa aaaattactc
    28081 gccctgttgg cgttgcagtc ttgctaatac atcctgggcg ctacttttat tgctaccgcc
    28141 aattccggct tccgccagct tttcgtcaag gtcgcgaccg tctgcgactt tttctaactg
    28201 tgcggcagca tccaggcgag cctgacgttc ggcctgacgc gtttgcaggc gttttaagga
    28261 ttctgctgct gtcgaaacgc tggaagatgc gccaacggta gaagtcgtta cagcctgttg
    28321 tgcacgctgc atggcttcag tggctttaac gacttccatt tgctgctcaa actgagcgat
    28381 acgctgcgct gtcgctgcaa ctgctttttc cacgccatca cgggagactt ccagattcga
    28441 caacacttgc tcttcagcgg taatgagatt ctcaaggcgc gcgatttctt cagcaacttc
    28501 gttaatcaac gacggattaa cgttcttgct taacgcttcc agcgtacggg cttccagact
    28561 ggctttgcgc tcacgtaaat ctttcagctt atcgtgactt aatttcaccc gcgccagcag
    28621 atcaacgcga gattttccgg ctttatcgag ttcagcttta gcgtcacgaa tatgctgttc
    28681 cagcatgcgc acaccctggg tttcttcaat ggattcttcc gcctgggaga taaacgattt
    28741 ccccagcgta aataaacttt ttaaaattcc cataaatacc cgtccttgta attagtgtga
    28801 atattcttct gtaatttcag ccagatccaa tgcgttatct accagcgagg ttatctcaag
    28861 caggatatct tcaagagaag atttaagcga taacgccccg aaaacaatgt aatactcttc
    28921 ttgttgtacg ctggagattc ctaccgatga caacggcatc atcttctgat ttcttaataa
    28981 gaaggtatta aattcatctg gattgctgat actactcacc ggacaaataa aggtttcgat
    29041 aatcatttgg cgggaagtaa aaagaatatt gatttgcaaa tcgccataat cattcatttt
    29101 aataattaat gcgttctcgc tatttgttac atcaatattt tgttcaggta cagtttgcag
    29161 cgccgtcgcc agcgccaacg gattccatgt catatattat tccatataga ttaagtttaa
    29221 atattaataa aatgaatatt tgcaatacgt aattatctta ccagctatag acaaaaaaaa
    29281 accatccaaa tctggatggc ttttcataat tcagaggaac tagctgcgct gacgaaccgc
    29341 ttcaaataag caaattccgg ttgcaaccga aacgttcagg gaagaaacac ttcctgccat
    29401 tgggatgctg atcaactcat cgcaatgttc acgggtcagg cgacgcatac cttcaccttc
    29461 cgcgcccatc accagcgcca ggcgtccggt cattttgctt tgatagagcg tatgatccgc
    29521 ctcacctgcc gtaccgacga tccagatatt ctcttcctgc aacatacgca tggtgcgcgc
    29581 aaggttagtc acccgaatca gtggaacgct ttctgccgcg ccgcaggcta cttttttcgc
    29641 cgtggcgttg agctgtgcgg agcgatcttt cggcacaatc accgcgtgaa cgccagcagc
    29701 gtccgcgcta cgcaggcacg cgccgaggtt gtgcggatcg gttacaccgt cgaggatcag
    29761 caggaacggt tgatcgagcg aagcgatcag atccggcaga tcgttttcct ggtactgacg
    29821 tcctggcttc acgcgggcga taatgccctg atgcacggca ccgtcgcttt tctcgtcgag
    29881 atattggcgg tttgccaact ggataaccac gccctgggac tcaagggcgt ggatcagcgg
    29941 taacagacgt ttatcttcac ggccttttaa aataaagact tcctgaaaac gttcaggggc
    30001 gcgctccagc agggcctgca ctgcatggat gccgtaaatc atttcgctca ttaatgtact
    30061 cgttgtttac gttttgcctg atgcactacg tttatccggt ctacatgatc cctgcaatat
    30121 attgaatttg cgtgattatg taggccggat aaggcgttca cgccgcatcc ggcatgaaca
    30181 aagcgcattt tgtcagcaat ctaaccctct tctgtttgaa gagggtaatg atcactctgc
    30241 cacttttttc ttcgccgcac gcttcgcttt ggtcgctgca gctattttct gcgttttcgc
    30301 cgatggcttt ttcgcttttc tcgcgtcttt cttcgctgct ttcggcttcg tttttttctc
    30361 accgcggaag gcgctgtctg gctcaaagtt taccttttta ccgacctgac gacgcttgcc
    30421 gcctttttta cctgcatcgc cttttttcgc tttctcgcgc gccgttttac cgacgttgcg
    30481 cggtgcgcgt tcgctggaga tcaggctaaa gtcgattttg cgctcgtcca tattaaccgc
    30541 ttcgacgcga acttccacgc gatcgcccag gcgataagtc tggccgctgg attcacccat
    30601 caggcgttgc cctacctggt caaagcgata gtagtcattg tccagcgaag agacatggac
    30661 cagcccatca atgaacaagt cgtccagacg gacgaagaag ccaaagccag tgacgctgga
    30721 aattacgcct ttaaagacgt tacctacctg gtcgagcatg aagtcacact tcagccagtc
    30781 agacacatcg cgcgttgctt cgtcggcacg acgttccgcc atcgaacagt gctgacccaa
    30841 ttgcagcatc tcttccatcg aataatggta gccaccggtt tcagtggtgt tgccctgatg
    30901 cccctgctct ttcgccagca gatacttaat ggcgcggtgc agcatcaggt caggataacg
    30961 acgaatcggc gaagtaaagt gcgcatagga ctgcaatgcc aggccaaagt gaccacggtt
    31021 ttccggatcg taaatcgcct gtttcatcga gcgcagcagc atggtttgca gcatttctgc
    31081 atcaggacgg tcggcaaccg actccagcag ctccgcgtag tcgcgcggtt ccggcttgtt
    31141 accgcccggc agttccagcc ccagctctgc cagcactgaa cggaaagagg taatcgcttc
    31201 ggtgctcggc ttgtcgtgaa tacggaacag tgccggttct ttcgctttct caacgaaacg
    31261 cgccgccgag atattcgcca gaatcatgca ctcttcaatt aacttgtgtg cgtcgttacg
    31321 ctgagtctgt tcgatacgtt caatacggcg ttcagcgttg aaaatgaact tcgcttcttc
    31381 gctctcaaat gagatcccgc cgcgttcttc acgggcttta tccagcactt tatagaggtt
    31441 atgcaactct tcgagatgct taaccagcgg ggcgtactgc tcgcgcagat cctgatcgcc
    31501 ctgcagaata tgccagactt tggtgtaggt cagacgcgcg tgagagctca tcaccgcttc
    31561 gtagaatttg tagccagtca ggcggccttt cgacgaaacc gtcatctcgc acaccataca
    31621 caggcggtct acctgcggat tgagcgagca cagaccgtta gagagcactt ccggcagcat
    31681 cgggataacc tgcgaaggga agtacaccga cgtaccacgg ttacgcgctt ctctgtccag
    31741 cggcgttggc ggacgcacat agtagctgac gtcagcaatc gcgacccata aacgccagcc
    31801 gccgccgcgt tttttctcgc agtaaaccgc atcgtcaaag tcacgggcgt cttcgccatc
    31861 aatggtgacc agcggtaaat cgcgcaaatc gacacggccc gcttttgctt cttccggcac
    31921 ttcttctttc agcccagcaa cctgttgctc aacagcctgc ggccagatgt acggaatttc
    31981 atgggtacgc agagcgatat caaccgccat gcccgtgccc atattgtcgc ccagcacttc
    32041 gacgatttta cccaccgctt tggtacggcg agtcgggcgt tgggtcagtt caacaaccac
    32101 cacaaagccc atccgcgcgc ccatgatctg atcgggcggg attaagatat cgaagctcag
    32161 acggctatcg tcaggaacca caaagccgac gcccgcttcg gtaaagtagc gaccaacaat
    32221 ctggctggtt tttggcacca gtacgcggac aatacgcgct tcacgacgac ctttacggtc
    32281 agcacccagc ggctgagcca gcacctgatc gccatgaatg caggttttca tctgctcgct
    32341 ggagagatac aaatcatctt tacgcccttc aacccgcaga aagccgtagc catcacggtg
    32401 gccaataacg gtacctttca ccaggtcgag gcgttccggc agcgcatagc actgacgacg
    32461 agtaaagacc agttgaccat cgcgctccat cgcgcgcagg cggcgacgca ggccttcaag
    32521 ctgctcttcg ccttcaatgt gcagttctac cgccagctca tcacggctgg ccggtttttc
    32581 acgtttggtt aaatgttcga ggataaattc ccgactaggg atgggattcg cgtatttttc
    32641 agcttcgcgt tcctggaaag gatcttgtga catctcggtt cctccgttgt catctctgat
    32701 gaagattttc gtcactccac cagcaataat ttataaagcg gttgattctc ttcaaccaaa
    32761 tcggcaagcg tgtagttatc cagttccgta agaaaacttt gcacggcctt agaaagcgcc
    32821 tgtttcaacc gacaggcagg tgtaatgtgg caaaactcac tgctgcaatt caccagcgat
    32881 aagggctcca gctcgcgcac cacatcacca atacgtatcg cactcgccgg tttacccagg
    32941 cgaatgccgc cattttttcc acgaacagca gtcacgtagc cggcacgact aagttgattg
    33001 attattttga ccatatgatt acgggagacg ccgtagacgt cagtcacttc agaaatactg
    33061 gtcatccgcc cttctggcaa tgacgccatg tagatcagcg cacgtaatcc gtaatcagtg
    33121 aaactcgtta actgcacatc aacctcaaaa gggaaatcgg gaaaaaatac atttatattg
    33181 atgataaacc agccacaagc tgtgtcgcta atttatttca gttcggggaa ggaaaaaagc
    33241 gaggatttaa cactgtgtcg gatagcgtaa tggcttaacc gacacagcaa tgtatcacac
    33301 tgttagcccg gcaggcaaaa tatctgccag gcgtaccaga attacgcgtc gaacgggtcg
    33361 cgcagaatca tggtttcagt acgatccgga ccggtagaga tgatatcgat cggtacaccg
    33421 gtcagctctt caatgcgctt gatgtagttc agcgccgcct gcggcaggcc gctacgatct
    33481 ttcacgccga aggtggattc agaccagccc ggcatggttt cgtaaatcgg ctctacacct
    33541 ttccagtcgt cagctgccag cggagtggta gtcacttcac gaccatccgg catacggtaa
    33601 gccacacaga gtttcacctc tttcaggcca tccagaacgt ccagtttagt caggcagaag
    33661 ccagacaggg agttcagctg taccgcacga cgaacggcaa cggtgtccag ccagccggta
    33721 cgacgacgac gacccgtagt tgcgccgaat tcgttaccct gcttgcagag gaactcgcca
    33781 gtttcatcaa acagttcagt cgggaacgga cctgcaccca cacgagtgga gtaagctttg
    33841 aggataccca gaacgtaatc aacataacgc gggcccaggc cggaaccggt cgccacgcca
    33901 ccagcagtgg tgttggaaga agttacgtac ggataagtac cgtggtcgat atccagcagc
    33961 gtaccctgcg caccttcgaa catgacgaaa tcgccacgct gacgcgcctg gtcgagcagg
    34021 tcagaaacgt caaccaccat agaagtcagg atgtcggcaa cagccatcgt atcatccaga
    34081 actttctggt aatcaaccgc ttcagctttg tagtagttaa ccaactggaa gttgtgatat
    34141 tccatcactt ctttcagttt ttcagcgaag gtttctttgt cgaaaaggtc gccaacacgc
    34201 agaccgcgac gtgccacttt atcttcataa gcaggcccga taccacgacc ggtggtgccg
    34261 atcgctttcg cgccacgcgc tttctcacgc gcgttatcca gcgcaacatg ataatcaagg
    34321 atcagcggac atgcttcaga cagcagcaga cgctcacgaa cggggatgcc acggtcttcc
    34381 agttctttca tctctttcat cagcgcggcc ggagacagca caacaccgtt accgatgatg
    34441 ctagttacat tctcgcggag aatacctgat ggaataagat ggagaacggt tttttcaccg
    34501 ttgattacga gagtatggcc tgcgttgtga ccgccctggt agcgtacaac atatttagcc
    34561 cgttcagtca gaagatcgac gatcttacct ttaccttcgt caccccattg ggtgcccagt
    34621 acgacgacgt tgttacccat ttttcaaaat caccgtttgc ttaaaaatgg attctaccat
    34681 cgctttttca gagttacagc actttttgca ctcaaaaatg acctcgatca gtctgttttt
    34741 tgttcagcca atcgttttcc tcaacatgta gtagaccaca acgcccgcaa ccacaagtcc
    34801 accgccaaaa cgacgtaaaa tattatcggg caaattggtc atcgcagaga tcatcttctt
    34861 ccatgccttc gggtaaagca tcggccctaa accttccagt accaaaacca gggcaagcgc
    34921 cagccagatt gtcgaattca ttcattatcc ttatagaaaa agaaaaccac cgacatccct
    34981 gaggatgcgg tggctttatt gacctgtacc gcagtcgtta tattaacgcg ttgcggaagt
    35041 cggcgtcttc atgtagcgga agaaatcgct atccgggctc atgaccatca cgtcctgatt
    35101 gccagagaag ctgttctcat aagcacgcag gctacggatg aatgcgtaga agtccggatc
    35161 tttactgaat gcatcagcaa acagtttggc tgcttcggca tcgccttcac cgcgcatgat
    35221 gcggccctga cgctcagctt ctgccagcgt tctggtcact tcatagtcgg cagtcgcgcg
    35281 cagtttttcc gcttcttcct gaccttgtga acggtgacga cgcgctaccg cttcacgctc
    35341 ggcgcgcata cggttgtaga tcgcttcaga cacttcggtc ggcaggttga tctgcttaat
    35401 tcgcacatcg acaacttcaa tacccagcgc cgccatactg ttcgggttga tgaccggaac
    35461 tttgcccttc gtctcagccg ttacgcgctc tgccgcttcg gcaatggcgt tatctgccgc
    35521 cggggtagta acttcatctt ctgtacccgc agaaccggag ttcagcgcgt cacgtacttc
    35581 gagggtcaga cgaccacggg aatcggtgac gatgtctttc acgtccaggc gaccaatttc
    35641 agaacgcaga cggtcagaga acttacgttt caacagcact tccgcttgcg aaatgtcgcc
    35701 cccccccgtt gccaggtagt aacggctgaa atcgctgatg cgccatttga tgtaagagtc
    35761 gacgatcagg tctttcttct ctttggtcac aaagcggtcg gcctggttgt ccatggtctg
    35821 aatacgtgcg tcgagcattt tcaccgtttc aatgaacggt atcttgaaat gcagacccgg
    35881 ctcataaacc agaggtttgt tgtcatcgtc acgcagtacc ttaccaaaac gcagcgtaat
    35941 accgcgctca ccttctttga cgacaaagac agacatgtaa agcactacca gcacgatgat
    36001 gataatcgcg ataactgact tacgcatcgt tattccccct gacgctggta gtcgttacgc
    36061 tgcgcattgg cgcggcgttg gtccataata tcgccctgac tggtggacga cgtgttgctt
    36121 gctccactgg ttgtggagga agaggctggc ggcagacgca gcagattgct ggcaccgtta
    36181 tcgctcttcg ccgcaggggc gttaccacct ttcagcatct ggtctaacgg cagaaccatc
    36241 aggttgccac ctttatcgtt aaccagcact ttgcgggtgt tacccaacac tttttccatc
    36301 gtctcgatat acagacgctc gcgagtaatt tccggcgcgg ctttatattc cggcagaagt
    36361 ttagcaaaac gcgccacttc accctgagct tccaggatgg tctgggcctt gtacgcacgc
    36421 gcctcttcga ggatacgttg cgcctgaccg ttcgcacgcg gctgaacttc gttggtatac
    36481 gcttctgctt cacgaatgta ttgctgttcg ttttcacgcg cggcaatcgc atcgtcaaac
    36541 gccgctttta cttcttccgg cggacgagca gcctggaagt tgacgtccag cagcgtgata
    36601 cccatgtcat acggacgaat cgtctcttcc agttcgcgct gagtatcgct acgaatcacg
    36661 gtacgacctt ccgtcagaat gcggtccatg gtgtatttac cgataactcc acgcagggcg
    36721 ctgtcggtag cctgacgcag gctgtcatcc gggctggtca cgctatacag atatttttcc
    36781 ggattggtga cgcggtactg cacgttcatc tcaacgcgca ctacgttctc gtccgacgtc
    36841 agcatcacac cagaagctgc cagttcacgc acggcttcca cgttcaccgg tttgacttcg
    36901 tcgataaacg tcggtttcca gttcagacct ggctcaacca gatggctgaa tttaccaaag
    36961 cgtgttacca cgccgcgttc ggcttcttta atggtataga aaccactggc cgcccagata
    37021 atgacaatcg ctgccgctgc gatggtaacg acacgaccgc caagctgcgg gcgcgggcct
    37081 tgcgatgaac tgccaccgcc agatccggtg cctttaccgc cgcccagacc accgagcttt
    37141 ttgctcagtt tgcggaagat atcatctaaa tcaggtggcc cttgatcgcg accgcctttg
    37201 tttccatttc cctcagagtt gccgccaggt ttgctgcttc cccacgggtc gcggtcttgt
    37261 ccgttattac cgggctgatt ccacgccatg tttgtgctcc atatttgtta tgcggtgatc
    37321 cctgttgtct ttcgacgcca gaggatatga ctccacgctt cagacgctac gccgttagat
    37381 caggtaatcg atcaacgccg gttcttgttt acagaggcga cgccagtcaa cgatcggcat
    37441 acgaacttgc agacttacgc tgccgtcctc ctccatccac tctttttcta ttgcctgaag
    37501 ctgataaaaa cgacttctca gacgcccttc ctgcggtggc agacgcaatg tatgctgcgc
    37561 cacctcgccg gaaagccgct ccgtcaaagc ctgaaaaagc tgtggtatcc ccgctccggt
    37621 ctgtgcggaa agccagacac ggatcggttt gttctcttcg tcccgatcaa tacgcggttc
    37681 gaaatcttcc agcatatcga tcttgttcat caccagcagg gttgggatct cgtgagcgtc
    37741 gatctcttca agaaccgtat tcaccgcttc gatgttttct tgtacacgca catccgccgc
    37801 atcaatcaca tgcagcagca atgtggcttg ccgcgtctct tgtaacgtgg ctttaaatgc
    37861 tgccaccaga tcgtgcggca ggtggcgaat aaaccctacg gtatctgcaa gtacggtttc
    37921 accgacatct gcaacgtcaa tacgccgcaa cgtcgggtcg agggtggcaa acaactggtc
    37981 tgccgcgtag acccgcgctt cggtgatgcg attgaaaagg gtagatttac cggcgttggt
    38041 atatcccacc agcgaaacag taggaacgtc ggctttgata cgcgattgcc gcccctgctc
    38101 acgctgcttt tcaactcttt ccaggcgcga ctgtatctgc acgatgcgat tacgcaacaa
    38161 acgacggtcg gtttcgagct gggtttcacc cggaccacgc aaaccaatcc cgcctttctg
    38221 tctttcaagg tgggtccagc cacgcaccag gcgtgtagcc agatggcgca gctgcgccag
    38281 ctcaacctgc aacttaccct catgggtacg cgcacgttgg gcgaaaatat ctaaaataag
    38341 gccggtgcgg tcgataacac gacactcgca caaacgctcc aggttacgct cttgcgccgg
    38401 gctcagggca tggtcaaaaa gaacgaccga agcacccgtc gctttgacag cttccgcaat
    38461 ttcaactgct ttaccttcac ctacaaaata ctttgggtgc ggcgctttac ggctaccggt
    38521 aatcacctgc aatgcttcga caccggcgga agagaccaga gattcaaact cctggaggtc
    38581 ttccatatct ttgtcttgcg taaaatagat gtgtaccagt accgcctgct caccagcatc
    38641 ataacggtca aacaagcgta taaccctcta aatagatcag cggggaacgc aggatcgctg
    38701 gctccccgtg taaaaaaaac agcccgaaac cttattcggt ttcttcgctg tcctgttgcg
    38761 cggaagtatt ctgcgcgctg ctaccatgat ggtagttact gctggtaccg ccaccggcgt
    38821 tgttactgtg atgagaaacc gggcgagacg ggacaacagt agaaatcgcg tgcttgtaaa
    38881 ccatctggct gaccgtgttt ttcaacagga tcacgaactg atcaaaagac tcgatttgcc
    38941 cttgcagctt aataccattc accaaataaa tagaaactgg aacacgttcc cgacgcagtg
    39001 cgttcaggaa cggatcttgt aaagattgcc ccttagccat tctctctttt ccttatatgc
    39061 ttatttgtac tttgaacctt tcgattctga aaaaattgcg cacgatacgt ctcaattgta
    39121 cacattcagc ctgcgatagc accaacaacc tgtaatactt cgtcacgcgc ctgttctggt
    39181 ttttcactgt caagccagtg aaccccttcc caaccacgca gccaggttat ctgccgcttc
    39241 gccaactgtc tcgtggcgca aacacctcga taaaccattt catcgtatga gatttcgcct
    39301 ccaaggtaag accacatctg gcgataaccc acgcaacgaa tggaaggcaa gtccgtatgc
    39361 aaatctcctc gggcaaaaag cgcccggact tctgcttcaa aacctgaagc caacatctga
    39421 tgaaaacgct gctcaattcg ttgatggagc agttcacggc tcgccggggc gatggcgaac
    39481 tgatgcacct gatacggtag agcgtctcct gacgtttgcg tcagttccgt taaagtttta
    39541 cccgaaatga aaaaaacttc cagtgcccgg gaaagccttt gtggatcatt tggatgaatc
    39601 cttgctgccg caaccggatc gatctcctga agttgacgat gcaatgactc ccaaccttgc
    39661 tctgccgcct gttgctcaat tctggcccgt acttccgggt ctgccgacgg tagcggcgac
    39721 aacccttcca gcaatgcctt gaaatacaac atcgtaccgc ccactaacag tgggatccgc
    39781 cccgccgcgg tgatatcggc catttccgcc agcgcatcgc ggcgaaaatc agcagccgag
    39841 taagcctgcg acggatcgcg aatatccagc aatcggtgcg gcgcggcgag taactcttca
    39901 gcgttcggct tcgccgtccc gatatccatc cctttgtaaa taagggcaga atcaacgctt
    39961 atcaactcta ctggtaaaat tttacgcagc tcaatggcta acgccgtttt accggaggcc
    40021 gtcggcccca tcaaaaaaat cgccttaggc aggctcgcct tactgatatc actcatcttt
    40081 cagggctttt atcgccggat gtaaatcaac agattgtaac agaccacccg gcggcgtttt
    40141 cacaagttgc ggacataacc gttccacgtc cgccagcagg gttatggcct gtgccattga
    40201 ccactgcgca tgttcgctca tcagatttcg tgcaatccac tgcgcaatat tgccaggttc
    40261 gaatacggac tgcttcgcta ggtagcctat cagttcagga atcaagattt gtaaattttg
    40321 ttggcgtaag ggtaaaggca cggccctgat ggtcacatgc tgtgcatctg actggaaatc
    40381 aatacccaat tccgccaggg cagactgcgc tttttctaat gccgattttt cttcgccaga
    40441 aacttttagc cgcaacggaa tcagcaacgg ctgggcgcaa acgggcgctt cacccggcgt
    40501 cagttgtacc tgacgcagcc aacgttctgc cactggcaag gctaaaagtg aaatgttgcc
    40561 gtcgcgctcc agcaacgcac agtcggaatg gacgatagtc agtacccgac caaaactctg
    40621 actgttcgcc gcaagtgcag gttcctgcgg ttccggcgct tttggttttt gcatcggcgc
    40681 gggcgtttgc aaaagctggc gatacacttc accttgctgt ttctggtaac ctggctgcgc
    40741 attcggccag ggggcagccg gacgactacc tgatgctggc gcaggagtgt agcgcggagc
    40801 tactggctca cgaactgccg gttcagcaaa gtgattgcgc cccgccgcca cgcggttttc
    40861 cggaatggga cgcggtgcag gttggggttc atcgtccagc ggtagcggcg tttccagttg
    40921 ctgttgtagc acgctcagca cgccctgata gataaagtca tgcaccagac gcgactgatg
    40981 aaaacgcact tcgtgtttgg cggggtgcac gttgacgtcc acctgatgcg ggtcgatctc
    41041 cagatacaac acaaatgcag gttgctgatc ggcccccagt ttgtcttcac aggcctggcg
    41101 gatcgcgtga ttgatcaggc gatcgcgcat catgcgacca ttcacgtagc aatactgaat
    41161 ttccgccagt gcgggcgtgg tgtgatttgg atcggccacc cagccgcgca gcgtgagatc
    41221 gccgtgttgc cattcaatcg ccagcgcttg ttcgagaaaa gcggtgccac aaatcgcgcc
    41281 taagcgccgt tctttttgcc cgccttccgg caccgcgcgg tactggcgta cgattttgcc
    41341 gttatgcgac aggttgatcg tgacgtcgaa acgcgccagt gcgatgcggc ggatgatttc
    41401 atcgatatgg ttaaattcgg ttttctcggt gcgcaggaat ttgcgccgcg ccggggtgtt
    41461 gtagaacagg tccagtacct ccagcgtcgt tcccaccgga tgcgccgccg gtttaaccgt
    41521 cacgtccata tcgcgccctt cggcataggc ctgccaggct tcctgctgtt ctgcggtgcg
    41581 tgaagtgagc gtcaggcggg aaaccgaact gatactcgcc agcgcctcac cgcgaaagcc
    41641 caggctgata atggcttcga gatcgtccag agaggcgatt ttactggtgg catgacgcgc
    41701 cagcgccagc gccagctcgt cttttttgat accacagccg ttatcacgaa tgcggataag
    41761 tttcgcccca ccgcgttcga tatcaatatc gatacgcgtc gcacctgcat cgaggctgtt
    41821 ttccaccagt tctttgacta ccgacgcagg tcgctcgacc acctcacctg cggcaatctg
    41881 gttcgccagt tgtggcggta agacctgaat tggcatcaat cgtccttagt ttggcagcgt
    41941 gcgatctggc gtcgtcaccg tactggcagt ttgtgccgtt gcaccctgcg gcgcagattg
    42001 catcggatgc gcaaggaaat aattgcgcag gcctttgtaa atggcttctg ccagctgttg
    42061 ttggtaatcg tcgctcgcca gcaaacgttc ttcgctgttg ttgctgataa aaccggtttc
    42121 gaccagtact gatgggatat ccggcgagcg cagaacgcca aggctggcgt gttctggtcg
    42181 acgtttatgt atttcgccaa tgcgttgcaa ctgactgatc atactggtcg ctacatcata
    42241 ccctacccgc tgggaatgac cgaactgtaa atccagcacc gcctggctta aataggggtc
    42301 agactgactg ttcgccagca catcacccgc cccgcccagc agctccgact gtttctcgtg
    42361 ctgctccagc cagctggcca tttcgctgtt ggcgcgacgg ttagagagca cccatacgga
    42421 agcgccagtc gcactgcggt tcggtgcggc atcagcgtga atcgacacga ggaaattggc
    42481 gttttgctta cgtgccacat cgctgcgccc catcaccgag ataaagtaat ccccgtcacg
    42541 ggttaaaacg cctttaaaca tcggatcgtc attgagcaaa gtacgcaatt tacgcgcgat
    42601 ggcgatggtg acatttttct cccgcgtacc accggggccg atagcgccag ggtcctgacc
    42661 gccgtgtccg gcatcaatag cgatgataat tttatcgcca ttgttagccg tcgcgcgcgc
    42721 tgccggacgc gttaccgtat tactgctgat aacacccgta gtgcggttac tttccgtttt
    42781 aaacggattg cgcgccggtt cgctgacgcg cggtgcgaca accgtaggcg tttcaacgcg
    42841 tttcacaacc acaggcggcg gaggaggcgg tggcggcgca tcggcgttaa tcgtaaagac
    42901 gacagtgtaa ttgctgccat tctgccgctt caccgcttcg gttttaccat tttcggtaag
    42961 atcgaccacc agccgtagcg tttgtgcatc tttaggcgtt ccggagcgaa tcgccttcac
    43021 cagattatta ccgctgaaca acaacggcag tccctgaatc acgcccgttt gtttgatatc
    43081 gagcgccacg gtgcgtttgc tttgatggct aaacgcataa tcaggatcgc caataaaact
    43141 caacgttatc cgcgcctgtt ggttgccgtt agaaacctga atatcagaga gcgtcgcggc
    43201 acccacctgc gcgcacagca gcagcaacgt cgctaccaac caatttctga tgcgatacat
    43261 catcccacca cctttcaaag gttaaccggc taaacgtgcc agcaacaatt cacccgcaga
    43321 ggaaaccgca ctcacgcgcg cctcacggcc ttgtgcctga taatcaatgt gtatttcgac
    43381 atccgggtca ggaagaacac ctgtaccttg ttgtggccac tccaccaggc agatggcatc
    43441 gttggcaaaa taatcgcgga tccccataaa ctccagctcc tcgggatcgg caaggcggta
    43501 caaatcaaag tgataaacca ttaaattgtc gagcgtatag ggttcgacca gcgtataagt
    43561 ggggcttttg acattaccct gatggcccaa agcctgtaaa aagccccggc taaaggtggt
    43621 tttacctgcg cctaaatcgc catacagata gattacggtt gcgccatcgc aggctttcgc
    43681 tacccgctcg cccaggtcta atgttgcctg ctcatcaggg agcggaatta ctcgattcat
    43741 catggttttt atcagtcact tccgggttaa caatacgctg tagcgtggaa aagagatcgg
    43801 ttgccagcat cccgcgcgtt ccaaaacgcg ccgccagtac gtcagctgcc gcaccgtgcg
    43861 cgacacagcc tgcacaggct gcatcatacg gcgacagttt ttgcccaagc aatgcgccaa
    43921 taataccaga gagcacatcg cccatgccgc cgcttgccat gcctgcattt ccggcatcaa
    43981 taatgcctaa agcgtcagga tgggcggcga ccacggttcc ggcacctttc agcaccgcta
    44041 cgccgccata acgttgtacc agacgtttgg cgcaatgtaa gcggtcactt tcaatttcag
    44101 cgacggaaca gcctaacaac cgtgcggcct cgccaggatg cggcgtgatc acgcgattgt
    44161 gacgcttatc gggattgatt gccagcaggt tcaatgcatc ggcatcccac aacatcggtt
    44221 tgcgaaaatt ctcaactttt tgcagggctt ttttccccca ctcttgctgg cccagaccgg
    44281 gaccaatcac caccacatcg gcccattcca ggctttcggt aagagagtcc atagtcagtt
    44341 catgcaccat caattctggt cgtgcagtca gcagcggcgc aatgttctca ctgcgggtca
    44401 gtactcggac taaaccagca ccagcacgta gcgccgcttc ccccgtcata cgaatagccc
    44461 ccgccgtgcc gtgatcgcca ccgataatca ccagccgccc gtgatcgcct ttatgcgaag
    44521 tcgggcgacg cggtttcagc cagtgagaaa gttgttctgc cgaaaaccgc tgaattttcg
    44581 tctcctgacc tgccagccag ctatccagcc ccagtgagtc aaaatgcagt tgtcctgtaa
    44641 catcccgcgc ttttccagtg agcaagcctg gtttcagcgc aataaaagtg atggtgtgat
    44701 cggcgttgat cactgcgcct ggcgtagcgc cggtttcagc cagcaggccg gaagggatat
    44761 caaccgccac aatcggcgca ggatgggaat tagcgtggtc gattaactgg ctaatggatt
    44821 cgcggggtgc ttgctgcaag ccggtaccga gcagcgcatc aacaatcaga gctaccgact
    44881 cgggccagac aatattcgaa gcatggatct cgcctcccgc gtttaaccat gcctcgcgtg
    44941 ccagcgcggc ctcttccggc aacggtttat cgctctcctg ggccaacaac gtgacctcaa
    45001 tgccgaccgc tttggccagt cgcgcgacca cgtagccatc gccgccgtta ttaccatgac
    45061 cgcacaacac cagccagtgg cgggcgtcag gatacgccga acgacacacc tggaatgcgg
    45121 cctcgccagc gcgaagcatc agctcataga gtgtgagccc cagcgcatct gccgcctcgc
    45181 gttctccgcg gcggatatcg tcggcgtgcc agacggtgtg tggtatactt acggggtttt
    45241 tcttcattgt atggtccgtc atgtcagagc ccctcgatct caatcagtta gcgcaaaaaa
    45301 ttaaacagtg ggggctggaa ctgggctttc agcaggtagg tattaccgat accgatctca
    45361 gcgagtccga gcccaaactg caagcatggc tggacaaaca ataccacggc gaaatggact
    45421 ggatggcacg tcacggtatg ctgcgcgctc gcccccatga gttattgccc ggtacgctgc
    45481 gcgtgatcag cgtgcggatg aattaccttc ctgctaacgc cgcatttgcc agcacgctga
    45541 aaaaccccaa actcggctat gttagccgtt atgcgctggg ccgtgactat cacaaacttc
    45601 tgcgcaaccg actcaaaaag ctgggcgaga tgattcagca acattgtgtt tcgctgaatt
    45661 ttagaccgtt tgtcgattct gcgcctattc tcgagcgccc gttagctgaa aaagctgggc
    45721 tcggctggac aggtaagcac tcacttatcc tcaatcgcga ggccggttcg ttcttctttt
    45781 taggcgaatt gctggtcgat attccgctgc ccgtggatca accagtcgag gaaggatgcg
    45841 gcaaatgcgt ggcctgtatg acgatttgcc cgaccggtgc catcgtcgag ccatataccg
    45901 tcgatgctcg ccgctgtatc tcttatctca ccatcgaact ggaaggggcg atcccggaag
    45961 agttgcgacc gttaatggga aaccgtattt acggttgcga tgactgccag cttatctgcc
    46021 cgtggaatcg ctattcacaa ctcactacag aagacgattt cagcccgcgt aagccgctac
    46081 acgcaccgga actcattgag ttattcgcct ggagcgaaga gaagttttta aaagtcacgg
    46141 aaggttcggc gattcgccgt ataggtcacc tgcgctggct gcgtaatatc gccgtggcat
    46201 taggcaatgc gccctgggat gaaacgattc tggcggcgct cgagagtcgc aaaggtgagc
    46261 acccacttct tgatgagcac atagcgtggg cgatggcgca gcaaatcgag aggcgaaatg
    46321 cgtgcatagt cgaagtgcaa ttgccgaaaa aacagcgtct ggttcgggta attgaaaaag
    46381 ggttaccgcg tgacgcctga ttcatccaca gcttgtgaat aaaaataaaa acgcattgca
    46441 attcaagacg aataaaatcg tcaagtgatc gactcgacaa aacgaaatct taatttttaa
    46501 attatctatc aataggttaa aaaaatacta ttcaccgtgc gaagtttctt tgaaattaag
    46561 attacggcct gaggctgtgg ataactctgt ttacaagagt ttgtcagaga caacaaaaac
    46621 cagcaataac gcatcgcgtc gctgtggata ttttattgag agaagaattt ggagcgggaa
    46681 acgagactcg aactcgcgac cccgaccttg gcaaggtcgt gctctaccaa ctgagctatt
    46741 cccgcttggg tggtctgtgc tttgcagcac ttttcaaatt ttggagcggg aaacgagact
    46801 cgaactcgcg accccgacct tggcaaggtc gtgctctacc aactgagcta ttcccgcgta
    46861 ctacttaatt ttgctttcgt aattttcgca tttctgcgtc gttacgggag gcgcattata
    46921 cgagaaatct ttccctctgc aaccccccta aaaatttttt tttgagtttt tatctcaagt
    46981 gctgaaatta tcagcaagac gttcaattta gcgtcaaaat cacgccgccg caagtggatt
    47041 ccggcgctta cgtgaccagc aaaaatttta cagcttgata aaatgctcgc ggtagtaagc
    47101 cagctccgcc accgattcac ggatatcatc catcgcctga tgcgtcccct gtttggtaaa
    47161 accatcaaga atttccggct tccagcggcg cgccagctct ttcagggtgc tgacatcgag
    47221 ataacggtag tggaagtagg cttccagctc cggcatgtat ttaaacagga agcgacgatc
    47281 ctgaccgatg ctgttaccgc aaatcggcga tttacccgca ggcacccact gttttaaaaa
    47341 ttcgagcgtt gccagttcag cttcacgatc gcccatcgtg ctcgctttca cgcgctccac
    47401 cagcccgctg gcggtatggg tgcgcacgtt ccagccatcc atcagcgcca gctgttcatc
    47461 ggactgatgt actgcaatgg tcggcccttc tgccagaata ttcagattgg catcggtcac
    47521 cagcgtggca atctcaataa tgcgatcgcg ctcgggatcc agaccggtca tctcaagatc
    47581 gatccaaatc aggttgtttt cattggcact catgctattt tccacccatt cacagtgact
    47641 atattgatcc acaattgcgt gtatcataga tgttttgccc atcaggggcg accaggagtc
    47701 agtacgattg agtaaaaata aactctccaa aggccagcag cgccgcgtga acgccaatca
    47761 ccagcgtcgt cttaaaacgt ctaaggagaa gcccgactac gacgacaatc tgtttggcga
    47821 gcctgatgaa ggtatcgtca tcagccgctt tggtatgcac gctgatgtgg aatccgccga
    47881 tggcgacgtt caccgctgca atattcgccg taccatccgt tcgctggtga ccggcgaccg
    47941 cgtagtctgg cgtccgggta aaccggcggc ggaaggcgta aatgtcaaag ggatcgtgga
    48001 agcggtgcat gagcgcacct cagtgttgac gcgtccggat ttctacgacg gcgtgaaacc
    48061 tattgccgcc aacatcgacc agattgtcat tgtctccgcc attttgccgg agctgtcgct
    48121 caatattatc gaccgttacc tggtggcctg cgaaaccttg cagattgagc cgattattgt
    48181 gctcaacaag atagacctgc tggacgacga aggcatggcg ttcgtcaacg aacagatgga
    48241 tatctaccgc aatatcggtt atcgcgtatt gatggtttcc agccatactc aggatgggct
    48301 aaaaccgctg gaagaggcgt tgaccgggcg catcagcatt tttgccgggc agtctggcgt
    48361 cggcaaatcc agcctgctga acgcgttgct ggggctgcaa aaagagatcc tgaccaacga
    48421 tgtctctgac aactcggggc ttggtcagca caccacgacc gccgcacgcc tgtatcactt
    48481 cccgcacggc ggtgatgtga ttgattcccc tggtgtgcgt gagttcggcc tctggcacct
    48541 ggagccggaa caaatcactc agggctttgt cgaattccat gactatttag gtctgtgtaa
    48601 atatcgcgat tgcaaacacg ataccgatcc gggctgcgct atccgggaag cggttgagga
    48661 aggaaaaatc gcggaaaccc gtttcgaaaa ctatcaccgt attctggaaa gcatggcgca
    48721 ggtaaaaacg cgtaaaaact tttctgatac ggatgactga caattaagct aaccgtcgtt
    48781 agaatcgtcc cctttttttc aggatccggc atttaggccg gatcaggaac gacaaaacaa
    48841 tggcctggag gctaccttgt taaattcatt taaactttcg ctacagtaca ttctgccgaa
    48901 actatggctt actcgcctgg cgggttgggg cgcaagcaag cgggcaggat ggctgacaaa
    48961 actggttatc gatctgttcg ttaaatacta caaggtcgac atgaaagagg cgcaaaagcc
    49021 ggacaccgcc agctaccgca cctttaacga attctttgtc cgtccgctgc gtgacgaagt
    49081 acgcccaatc gataccgatc cgaatgtcct ggtcatgcct gccgatggcg ttatcagcca
    49141 gttgggtaaa atcgaagaag ataaaatcct gcaagccaaa ggccacaact acagcctcga
    49201 agccctgctg gcaggcaact atctgatggc ggacctgttc cgcaacggta cgtttgtgac
    49261 cacttacctc tccccgcgtg actaccaccg cgtacacatg ccgtgcaacg gtattctgcg
    49321 tgagatgatc tacgtgccgg gcgatctctt ctccgttaac catctcacgg ctcagaacgt
    49381 gccgaatctg tttgcccgta acgaacgcgt gatctgcctg ttcgataccg aatttggccc
    49441 aatggcgcag attctggtcg gggcgacgat tgtaggcagc attgagacgg tctgggcggg
    49501 caccattacg ccgccgcgcg aaggcatcat caagcgctgg acctggcctg ccggggaaaa
    49561 cgacggttct gtggcgctgc tgaaaggcca ggaaatgggt cgctttaaac ttggctctac
    49621 cgttatcaac ctgtttgcac cgggtaaagt gaatctggtt gagcaactgg aaagcctgtc
    49681 tgtcacgaaa attggccagc cgctggcagt atctaccgaa acctttgtta cgccagacgc
    49741 tgaacccgcc ccgcttcctg ctgaagagat cgaagcagaa cacgacgcca gcccattggt
    49801 tgacgataaa aaagaccagg tctaatccat caaaggaaac gctgacgtgc gcctgattat
    49861 cacttttctg atggcctggt gcctcagttg gggggcgtac gccgcgacgg cccccgatag
    49921 caaacaaatc acccaggaac tggagcaggc aaaagcggcg aaacccgcgc agccagaagt
    49981 cgtagaggca ctccagtctg ccttaaatgc gcttgaggaa cgaaaaggtt cccttgagcg
    50041 catcaagcaa tatcaagaag tcattgataa ttatccgaaa ctctccgcta ctctgcgcgc
    50101 acaattaaac aacatgcgtg acgagccgcg cagcgtgtcg ccggggatgt ctaccgacgc
    50161 gctgaatcag gaaattctcc aggtcagcag tcagttgctg gataaaagcc gtcaggccca
    50221 gcaagagcag gagcgcgccc gcgagattgc cgattcgctg aatcaactgc cgcaacagca
    50281 aaccgacgcc cgccgtcagt taaatgagat cgagcgccgc ctggggacgc ttaccggcaa
    50341 tactccgctc aatcaggcac aaaatttcgc gttgcagtct gactctgcac gtcttaaggc
    50401 gctcgttgat gaactggagc tggcgcagct gtctgccaat aaccgccagg aattagcgcg
    50461 cttgcgctct gagctggctg aaaaagagag ccagcaactg gatgcgtatt tgcaggcctt
    50521 gcgtaatcaa ttgaacagcc aacgccagct tgaggcagag cgggcgctgg aaagtaccga
    50581 attactagca gaaaacagtg ccgatttgcc gaaagatatc gtcgcgcaat tcaaaattaa
    50641 ccgcgaacta tcggcggcgc tgaatcaaca ggcgcagcgg atggatctcg ttgcctcgca
    50701 acagcgtcag gctgccagcc agacgttaca ggtccggcag gcgttgaata cgctgcgtga
    50761 acagtcgcaa tggctgggat cgtccaatct gctcggtgaa gcgttgcggg cgcaggtggc
    50821 acggctgccg gaaatgccga aaccacaaca gcttgatacc gaaatggcgc agttgcgtgt
    50881 gcaacggtta cgttatgagg atctgcttaa taaacagccg ctgctacggc aaattcatca
    50941 ggccgacggt cagccgctga ccgctgagca aaaccgtatt ctggaagcac agctacgcac
    51001 tcagcgtgag ttgctgaact cattgttgca gggtggcgat acgctactgc tggaactgac
    51061 caagctgaaa gtctccaacg ggcaactgga agatgcgctg aaagaagtga acgaagcgac
    51121 ccaccgctat ctgttctgga cctctgacgt gcgcccgatg accatcgcct ggccgctgga
    51181 aatcgcccag gatctgcgtc gtctcatttc gctggacacc ttcagtcagt tgggcaaagc
    51241 cagtgtgatg atgctgacca gcaaagagac gattttgccg ctgtttggcg cgttgattct
    51301 ggtcggttgc agtatttact cgcgccgcta tttcacccgt tttcttgaac gttcggcggc
    51361 gaaagtcggc aaagtgactc aggatcactt ctggctgacg ttgcgcactc ttttctggtc
    51421 gattctcgtc gcgtcaccgc tgccggtgct gtggatgacg ctgggttacg gcttgcgcga
    51481 ggcgtggcct tatccgctgg cggtcgcgat tggtgatggc gtcacagcca ccgtgccgct
    51541 gctgtgggta gtgatgattt gcgctacctt tgcccgcccg aacggcttgt ttatcgctca
    51601 ttttggctgg ccgcgcgaac gtgtttctcg tgggatgcgc tactacctga tgagcatcgg
    51661 gcttattgtg ccactgatta tggcgctgat gatgttcgat aacctcgacg accgcgaatt
    51721 ttccggttcg ctgggacggc tttgctttat cctcatttgc ggtgcgctgg cggtggtcac
    51781 cctcagcctg aaaaaggccg ggatcccgct gtatctcaac aaagaaggca gcggcgacaa
    51841 tattaccaac catatgttgt ggaacatgat gattggcgcg ccactggttg ccattctggc
    51901 gtcggcggtg ggttatctgg caacggcaca ggcgctgtta gcgaggcttg aaacctcggt
    51961 tgccatctgg ttcctgctac tggtggttta tcacgttatc cgccgctgga tgctgatcca
    52021 acgacgcagg ctggcgtttg accgggcgaa gcatcgccgg gcagagatgt tagcgcaacg
    52081 tgcgcgtggc gaagaggaag cacatcatca cagtagcccg gaaggggcaa ttgaagtcga
    52141 tgaaagcgaa gtcgatctcg atgccatcag tgcgcagtcc ttgcggctag tgcgctcaat
    52201 tttgatgttg atcgccctgc tttcggtcat tgtgctgtgg tcagaaatcc attccgcttt
    52261 cggcttcctc gaaaatattt cgctgtggga tgtcacctcc acggtacagg gcgtagaaag
    52321 tctggagccg attaccctcg gtgcggtgct gattgccatt ctggtgttta tcatcaccac
    52381 gcagctggtg cgcaatctgc ccgcgctgct ggaactggcg attttgcagc acctggattt
    52441 aacgccgggt accggctacg ccatcaccac catcaccaaa tatctgctga tgctgattgg
    52501 cgggctggtc ggcttctcga tgattggtat tgagtggtcg aaattgcagt ggctggtcgc
    52561 cgcgctcggt gttggtctcg gttttggttt gcaggaaatt ttcgccaact ttatctctgg
    52621 cctgatcatc ctgttcgaaa aaccgattcg cattggcgat acggtgacaa ttcgcgatct
    52681 tactggtagc gtgacgaaaa ttaatacccg cgcaaccacc atcagcgact gggaccgtaa
    52741 agagataatc gtgccgaaca aggcgtttat taccgagcag tttatcaact ggtcgctctc
    52801 tgactcggtc acgcgcgtgg tgttgacgat tccggcccct gccgatgcca acagtgaaga
    52861 agtgacggaa atcctgctca ccgcagcgcg tcgctgctcg ctggtgatcg acaacccggc
    52921 accggaagtc ttcctggtgg atctgcaaca ggggattcag attttcgagc tgcgtattta
    52981 cgccgctgag atgggtcacc gtatgccgct acgccatgag atccaccagc tgattctggc
    53041 tggcttccat gcccacggta tcgatatgcc attcccgccc ttccagatgc gtctggaaag
    53101 cctcaacggt aaacaaacgg ggagaacgct gacgtctgcg ggcaaaggtc gtcaggcggg
    53161 aagtttgtaa tgcatgaagc tgccaaatga tgtcaacgtg gctcatttag cagcttaaca
    53221 tcatcttcag ttatcaccgg ccctggagag tatcatccat acgcattact tcctgacaac
    53281 tcatcgccgg agagacgatg agttgcagga tttcatatga tcagtcgaga gctaactcag
    53341 gtttattcgc catgcgactg caatagttct ggtaaatcgc catcgctaac agcgagaaga
    53401 agacagggcc gccaatcatc cacaatgtgc tgtcccagtc tccggcttca accacaggct
    53461 gaataatggt gaagacgttc gcaaatgtca ccaccagaac gacaaccacc gtcgcaatca
    53521 ttgccgacat acgcgttttg aaaatcacaa atggtctgtc gagatcctga cgtgctttaa
    53581 agaatgggaa agccagcgcg aggaacaggt aaggaagcgt catagacacg ttcgccatca
    53641 gcgtcagctt gttaaagaac gccgatgcgg taccgccacc aaacgaaacc agcaggatga
    53701 agacagtaac caacccgcac tgcatccaca tagcgataga cggcatcccc atcgcattca
    53761 ggcgcgtcat cggttccggc cacaacgctt tcggcgtccc ctggatgatg gctttcagcg
    53821 gtgaatagca aagcgtaaag aacgcaccgg tataagcgag gaacatcgaa agtccggtaa
    53881 tacgcgcaaa ccatacgccc agcgacaatg acgcttcagg tgacaaatgc agtgcgttac
    53941 ccagcgtcac cccgaggctc ttcatcagca cataggtaat attgccgagg ttaacggaac
    54001 cattacttaa tacctgctgc cagtttgtgc tgacgcccca taaaaatatt gccagcgaat
    54061 aaccgattga aataacaata gcggcaaaaa caataccttt ggcaaagttc ttttctggat
    54121 tttccgtttt atcgaccaga ccaccaaccg cttcaattcc gccataggca aaaatagcaa
    54181 ataccacaaa cgatagcatt gccagaccgg actgataacc tgggttcggt gatgcaagga
    54241 aattaatatc ctgcgcgaaa tgcccaccat ttaataacaa aatagtaatg cttactaaca
    54301 gcaatactaa attcagacac attactgcaa taccgcccac cgcagtaatg cgggcaattt
    54361 tattaatccc ctttgaagca acgacggtga ccagaatcat ccatgccact gccagcagac
    54421 caaccacctg cgtaggctcc agtccggcaa tacgccagtg ctgggtcatg tcgctaccat
    54481 agaggaatgt tgagaacggt acccaaactt tcgcggaggt gctcaccatc cagatgatat
    54541 aagaggaaaa ccacataaac gtaccaatga aggcaaaacg tggtccgaca ctattattca
    54601 tccaggaata gataccgcct tcttctttgc gataagcagc tcccatttca gccatcatta
    54661 aggcgaatgg aataaagaat aacaatgcag aaaatatata aaagggaatc gcactataac
    54721 ccattaagta ataagccgat gggctattgg caaatccaaa tacggaagta aagatcatca
    54781 atatgagtcc tatcagactc atctttttta tcgtgtgagg catgaaacca tccttcattt
    54841 cagcgcgtgt ataaaacgcg cccactgaac tgcactgccc aacattgagc gggcagcgta
    54901 aaaaccaata cagaattgat acctggatga tatcaaatat ccagtcttaa attgtggcct
    54961 ttagcggaaa aatgaaaaca tatgctacag aatggcgctt atcacgccat tcttcgctgt
    55021 taattcagta atttttcaga attatgcccg gtcaacgcta aaggcgatga cttcagccag
    55081 tgtctccgcg cccagcgcca acatcaccag acgatcaaca cctaatgcca cgccggaaca
    55141 gtcaggcata ccgactttca aggcttcaat cagattctga tcaatggggt gctgcggcaa
    55201 accgcgcgcc gcgcgcttac ggttatcttg ttcaaagcgt tgttgctgct cacgggcatc
    55261 cgtcaattca tggaaaccat tcgccagctc aataccttta taataaacct caaagcgttc
    55321 agcgacccga tgatcttcgg tactgatttg cgccagtgat gcctggctgg ctggaaagtg
    55381 gtacacaaag gtcggttttt ctttaccaat atttggttct acgccaaagg taaacagcaa
    55441 ttgtagcaac gtgtcgcggt cttcttcggt atcagcaaca ttgctcaaat ccagtttcgc
    55501 tgccacttcc cgcagttgcg ttttgtcggc agagagcggg tcaatttcca gataacgcaa
    55561 gaaagcttgt tgataagaaa ggctttctgc tgccgggcag tccagcacct gttgtaagag
    55621 atcgtccacc tcgttcatca accggtacat atcatagtgc ggtcgatacc actcaagcat
    55681 agtgaactca gggttgtgat aacgccccat ctcttcatta cggaaactgc ggcacagctg
    55741 gaataccggc ccgcaaccgg caaccagcag gcgtttcata tggtattccg ggctggtcat
    55801 taaccagaga ttcatcccct gcgaatgccc ggggccaacg aaacgtgtct caaacgggac
    55861 caaatgaata tcggttaccg tcgcctggct catacaaggc gtctccacct ccagcactcc
    55921 acgatcggca aagaaacgac ggatctccgc cataatcgcc gcgcgtttta ataagttagg
    55981 aatggatgcg ctcggctgcc aggatgccgt ttcgctcata gttaaatctc cagtttttga
    56041 caagggcacg aagtctactc gcaacgcgac ggcgagacaa attttacgca ggaataaaac
    56101 aacggtgggc agtgactaaa aaaagcacga tctgatggtt tagtaattaa attaatcatc
    56161 ttcagtgata atttagccct cttgcgcact aaaaaaatcg atctcgtcaa atttcagact
    56221 tatccatcag actatactgt tgtacctata aaggagcagt ggaatagcgt tcgcagaccg
    56281 taactttcag gcacttaccc tgaagtacgg ggctgtggga taaaaacaat ctggaggaat
    56341 gtcgtgcaaa cctttcaagc cgatcttgcc attgtaggcg ccggtggcgc gggattacgt
    56401 gctgcaattg ctgccgcgca ggcaaatcca aatgcaaaaa tcgcactaat ctcaaaagta
    56461 tacccgatgc gtagccatac cgttgctgca gaagggggct ccgccgctgt cgcgcaggat
    56521 catgacagct tcgaatatca ctttcacgat acagtagcgg gtggcgactg gttgtgtgag
    56581 caggatgtcg tggattattt cgtccaccac tgcccaaccg aaatgaccca actggaactg
    56641 tgggggtgcc catggagccg tcgcccggat ggtagcgtca acgtacgtcg cttcggcggc
    56701 atgaaaatcg agcgtacctg gttcgccgcc gataagaccg gcttccatat gctgcacacg
    56761 ctgttccaga cctctctgca attcccgcag atccagcgtt ttgacgaaca tttcgtgctg
    56821 gatattctgg ttgatgatgg tcatgttcgc ggcctggtag caatgaacat gatggaaggc
    56881 acgctggtgc agatccgtgc taacgcggtc gttatggcta ctggcggtgc gggtcgcgtt
    56941 tatcgttaca acaccaacgg cggcatcgtt accggtgacg gtatgggtat ggcgctaagc
    57001 cacggcgttc cgctgcgtga catggaattc gttcagtatc atccaaccgg tctgccaggt
    57061 tccggtatcc tgatgaccga aggttgccgc ggtgaaggcg gtattctggt caacaaaaat
    57121 ggctaccgtt atctgcaaga ttacggcatg ggcccggaaa ctccgctggg cgagccgaaa
    57181 aacaaatata tggaactggg tccacgcgac aaagtttctc aggccttctg gcacgaatgg
    57241 cgtaaaggca acaccatctc cacgccgcgt ggtgatgtgg tttatctcga cctgcgtcac
    57301 ctcggcgaga aaaaactgca tgaacgtctg ccgttcatct gcgaactggc gaaagcgtac
    57361 gttggcgtcg atccggttaa agaaccgatt ccggtacgtc cgaccgcaca ctacaccatg
    57421 ggcggtatcg aaaccgatca gaactgtgaa acccgcatta aaggtctgtt cgccgtgggt
    57481 gaatgttcct ctgttggtct gcacggtgca aaccgtctgg gctccaactc cctggcggaa
    57541 ctggtggtct tcggccgtct ggccggtgaa caagcgacag agcgtgcagc aactgccggt
    57601 aatggcaacg aagcggcaat tgaagcgcag gcagctggcg ttgaacaacg tctgaaagat
    57661 ctggttaacc aggatggcgg cgaaaactgg gcgaagatcc gcgacgaaat gggcctggct
    57721 atggaagaag gttgcggtat ctaccgtacg ccggaactga tgcagaaaac catcgacaag
    57781 ctggcagagc tgcaggaacg cttcaagcgc gtgcgcatca ccgacacttc cagcgtgttc
    57841 aacaccgacc tgctctacac cattgaactg ggccacggtc tgaacgttgc tgaatgtatg
    57901 gcgcactccg caatggcacg taaagagtcc cgcggcgcac accagcgtct ggacgaaggt
    57961 tgcaccaagc gtgacgacgt caacttcctc aaacacaccc tcgccttccg cgatgctgat
    58021 ggcacgactc gcctggagta cagcgacgtg aagattacta cgctaccgcc agctaaacgc
    58081 gtttacggtg gcgaagcgga tgcagccgat aaggcggaag cagccaataa gaaggagaag
    58141 gcgaatggct gagatgaaaa acctgaaaat tgaggtggtg cgctataacc cggaagtcga
    58201 taccgcaccg catagcgcat tctatgaagt gccttatgac gcaactacct cattactgga
    58261 tgcgctgggc tacatcaaag acaacctggc accggacctg agctaccgct ggtcctgccg
    58321 tatggcgatt tgtggttcct gcggcatgat ggttaacaac gtgccaaaac tggcatgtaa
    58381 aaccttcctg cgtgattaca ccgacggtat gaaggttgaa gcgttagcta acttcccgat
    58441 tgaacgcgat ctggtggtcg atatgaccca cttcatcgaa agtctggaag cgatcaaacc
    58501 gtacatcatc ggcaactccc gcaccgcgga tcagggtact aacatccaga ccccggcgca
    58561 gatggcgaag tatcaccagt tctccggttg catcaactgt ggtctgtgct acgccgcgtg
    58621 cccgcagttt ggcctgaacc cagagttcat cggtccggct gccattacgc tggcgcatcg
    58681 ttataacgaa gatagccgcg accacggtaa gaaggagcgt atggcgcagt tgaacagcca
    58741 gaacggcgta tggagctgta ctttcgtggg ctactgctcc gaagtctgcc cgaaacacgt
    58801 cgatccggct gcggccattc aacagggcaa agtagaaagt tcgaaagact ttcttatcgc
    58861 gaccctgaaa ccacgctaag gagtgcaaca tgacgactaa acgtaaaccg tatgtacggc
    58921 caatgacgtc cacctggtgg aaaaaattgc cgttttatcg cttttacatg ctgcgcgaag
    58981 gcacggcggt tccggctgtg tggttcagca ttgaactgat tttcgggctg tttgccctga
    59041 aaaatggccc ggaagcctgg gcgggattcg tcgacttttt acaaaacccg gttattgtga
    59101 tcattaacct gatcactctg gcggcagctc tgctgcacac caaaacctgg tttgaactgg
    59161 caccgaaagc ggccaatatc attgtaaaag acgaaaaaat gggaccagag ccaattatca
    59221 aaagtctctg ggcggtaact gtggttgcca ccatcgtaat cctgtttgtt gccctgtact
    59281 ggtaaggagc ctgagatgat taatccaaat ccaaagcgtt ctgacgaacc ggtattctgg
    59341 ggcctcttcg gggccggtgg tatgtggagc gcaattattg cgccggtgat gatcctgctg
    59401 gtgggtattc tgctgccgct ggggctgttt ccgggtgatg cactgagcta cgagcgcgtt
    59461 ctggcgttcg cgcagagctt cattggtcgc gtattcctgt tcctgatgat cgttctgccg
    59521 ctgtggtgtg gtttacaccg tatgcaccac gcgatgcacg atctgaaaat ccacgtacct
    59581 gcgggcaaat gggttttcta cggtctggct gctatcctga cagttgtcac gctgattggt
    59641 gtcgttacaa tctaacgcat cgccaatgta aatccggccc gcctatggcg ggccgttttg
    59701 tatggaaacc agaccctatg ttcaaaacga cgctctgcgc cttattaatt accgcctctt
    59761 gctccacatt tgctgcccct caacaaatca acgatattgt gcatcgcaca attaccccgc
    59821 ttatagagca acaaaagatc cccggtatgg cggtggcggt aatttatcag ggtaaacctt
    59881 attactttac ctgggggtat gcggacatcg ccaaaaagca gcccgtcaca cagcaaacgt
    59941 tgtttgagtt aggttcggtc agcaaaacat ttacgggcgt gcttggtggc gacgctattg
    60001 ctcgagggga aatcaagtta agcgatcccg caacaaaata ctggcctgaa cttaccgcta
    60061 aacagtggaa tgggatcaca ctattacatc tcgcaaccta cactgctggc ggcctgccat
    60121 tgcaggtgcc ggatgaagtg aaatcctcaa gcgacttgct gcgcttctat caaaactggc
    60181 agcctgcatg ggctccagga acacaacgtc tgtatgccaa ctccagtatc ggtttgttcg
    60241 gcgcactggc tgtgaagccg tctggtttga gttttgagca ggcgatgcaa actcgtgtct
    60301 tccagccact caaactcaac catacgtgga ttaatgtacc gcccgcagaa gaaaagaatt
    60361 acgcctgggg atatcgcgaa ggcaaggcag ttcatgtttc gccaggggcg ttagatgctg
    60421 aaacttatgg tgtgaagtcg accattgaag atatggcctg ctgggtacga agcaatatga
    60481 atccccgtga tatcaacgac aaaacacttc agcaagggat acaactggca caatctcgct
    60541 actggcaaac cggcgatatg tatcagggtc tgggctggga aatgctggac tggccggtaa
    60601 atcctgacat catcgttaac ggcagtgaca ataaaattgc actggcagca cgccccgtaa
    60661 aagcgattac gcctccaact cctgcagtac gagcatcatg ggtgcataaa acgggggcga
    60721 ccggcggatt tggtagctat gtcgcgttta ttccagaaaa agagctgggt atcgtgatgc
    60781 tggctaacaa aaactatccc aatccagcga gagtcgccgc cgcctggcag attctcaacg
    60841 ctctacagta aaatttcatc gggtccgaat tttcggacct tttctgcgct tttccttgct
    60901 gtcatctaca cttagaaaaa accagtaagg aaacatttat gcgcctgctc cctctcgttg
    60961 ccgcagcgac agctgcattt ctggtcgttg cctgcagttc tcctacgccg ccgcgtggcg
    61021 tgaccgtagt gaataatttc gacgctaaac gctatctcgg tacctggtat gagattgccc
    61081 gttttgatca ccgctttgaa cgtggactgg aaaaagtcac cgcaacatac agcctgcgtg
    61141 atgacggcgg cctgaatgtc attaataaag gctataaccc tgacagagga atgtggcagc
    61201 agagtgaagg gaaagcgtac tttaccggcg caccaactcg cgctgcgctg aaagtgtcat
    61261 tctttggtcc tttctatggc ggttataacg ttattgcact cgatcgggaa taccgccatg
    61321 cgctggtttg cggaccggac cgcgactacc tgtggatact ctcccgcacg ccaaccattt
    61381 ctgacgaagt gaaacaggag atgctggcag tcgcgacccg ggaagggttt gatgtcagta
    61441 agtttatttg ggtacagcag cccggtagtt agtgagtgct gagtttcaga ccaataatcc
    61501 ccaatacgat taacgccaga ctcgccagac gcatcgggtt agcggactca ccgagcagca
    61561 caatgccggt gatggccgcg ccgactgcgc caatacccgt ccacacggca taagccgtcc
    61621 ctactggtaa cgatttcatc gcccaggcaa gtagcgccat actgacaatc atcgccgtca
    61681 cggtaataac actcggcgtc aaacgactaa agccgtgggt atatttcagg cctacggccc
    61741 ataccacttc cagcagacca gcaataacta agataatcca ggacatatca ggctccggag
    61801 aattggggcc gtccccggtg aaaagacgcg tttgcaggtc gtcctgcaaa gctgatggtt
    61861 gaacggatat tttgccaggt ggcgggggaa tttcaactat tacctttgtg actatcgcag
    61921 aaaaaacaaa cggcacgaca cagctgtcgt accgttattg cttattgctg cgctttcgtt
    61981 gctgcaccag aaatcgcgtt accgccatca gaaatgtctt caccaacgcc acgcgtggtg
    62041 ttgcaggcag ttaatactgt tgaaagcacc agaacagaaa agatcgctgc aattgtcttc
    62101 ttcaccataa cgtcttcctt ttaggtaatg ttatttatgt ttgcctatag caaattaata
    62161 atagacaaca tcgccaaaaa tgatggaaat ctgatgattt ttcggaagag aagaaaaatt
    62221 agctggcagc gcgggagatg gagttgccga gatgtttgat gtcttcgccg aaaccgcgag
    62281 cggtgttaca gcccgtgagc agcgtgctgg caagcaaaac aagaacgata aggcgtttca
    62341 tcatctctga cctgaataag tgatggtgca gcctgcaggc cgcaccacaa ccgcattact
    62401 tcacgcgaga gacgtattca ccagagcggg tatccacttt gatgacttcg ccgatttgta
    62461 cgaacagcgg aactttaacc acagcgccag tagacagggt agccggtttg ccgccagtac
    62521 ctgcggtatc acctttcagg cccggatcgg tatcaacgat ttccagttca acgaagttcg
    62581 gcggagtaac ggagatcggc tgaccattcc acagagttac gatacactct gcctgatcca
    62641 gcagccattt agcgttgtcg ccaattgctt ttgcgtcagc agacagctgc tcgaaagttt
    62701 cgttgttcat gaagtgccag aactcaccgt cgttgtacag gtaagtcagg ttcatatcga
    62761 caacatcagc gccttcagcg gaatcagtag atttgaaggt tttttctacg cgagtaccgg
    62821 tcagcagacg acgcagttta acgcgagcaa atgcctggcc tttacccggt tttacgaatt
    62881 cactcgcttc aaccgcgtaa ggttcgccgt ctaacatgat tttaagacca gcacgaaaat
    62941 cgttgctata gtacgttgcc ataaggccct ctgaaatttg ttaattggta gctaagccac
    63001 aaaatggcgc atattgtaac cctaaatacc ccatccagag aagattggtt aacgcaactt
    63061 gccgatgttg tgaccgatcc tgatgaactt ctgcgtcttt tgaatataga cgcggacgaa
    63121 aaactgttgg ccggacgcaa cgccaaaaag ctgtttgccc tgcgtgtgcc ccgctcattt
    63181 atcgatcgca tggagaaagg caatccggac gatccgcttt tgcgtcaggt acttacctcg
    63241 caagatgagt ttgtcgtcgc gcccggattc tccaccgacc cgctggaaga acagcacagc
    63301 gtagtgcctg gtttgttgca taaataccac aaccgggcgc ttttgctggt caaaggcggc
    63361 tgcgcggtaa attgccgcta ttgcttccgt cgccacttcc cctatgccga aaatcagggc
    63421 aacaagcgta actggcaaac tgcacttgag tatgttgctg cgcatccgga actggacgag
    63481 atgattttct ccggcggcga tccgctgatg gcgaaagatc acgagctgga ctggttgctc
    63541 acacaactgg aagccatccc gcatatcaaa cgtctgcgga ttcacagtcg tctgccgatt
    63601 gtgatcccgg cacgcatcac agatgcgctg gttgaacgct tttcacattc tacgctgcaa
    63661 atcttgctgg tgaatcacat caaccatgcc aatgagatag atgaaacatt ccgtcaggcg
    63721 atggctaagt tgcgccgggc gggcgttact ttgctgaacc agagcgttct gttacgtggt
    63781 gtgaacgata acgcacaaac gctggcaaac ctgagtaatg cgttgttcga tgcaggcgtg
    63841 atgccgtatt acctgcatgt gctcgataaa gtacagggcg cggcgcattt tatggtgagt
    63901 gatgacgaag cacggcagat tatgcgtgag ttgctgacac tggtgtcggg atatctggtg
    63961 ccgaaactgg cgcgagaaat tggcggcgaa cccagcaaaa cgccgctgga tctccagcta
    64021 cgccagcagt aactgattaa acagggaagt ttaatctttg cccgttacat aacctttaat
    64081 tattcatata cattgctgtt gttactcttt atataaccaa caccaataat taacctttct
    64141 tttctgttta tatagagtga gtacggctat ttatgttatt ttaacaacta ttactaccaa
    64201 gcccatttat ctggcttata aattaaaatt aattatttaa ttgagaaatg gttagggaga
    64261 acctacatgg ccataagcat caagggtgtt aataccggtg ttattcgcaa gagtaataat
    64321 ttcattgcac tggcgctgaa aattaaagaa cctcgcaata aagaatcgtt gttctttatg
    64381 tctgtcatgg aactgcgcga cctgctgatt gcgctggaaa gtcgtctgca tcaaaagcat
    64441 aaactggatg ctgctgcacg ccttcagtat gagcaagctc gcgataaagt cattaagaaa
    64501 atggcagaaa acatcccaga aattctggtt gatgagctta aaaatgccga tattaatcgt
    64561 cgggttaaca cgctggaact taccgataat caaggcgaaa atcttacctt cgtattaacg
    64621 ctacatgacg gtagcaaatg tgagttagtc gtcaatgagt tgcagattga aatgctggcg
    64681 cgagcaatca ttcatgcaat taataatgcg gaaatgcgtg aactggcatt acgtattacc
    64741 tcaccgttag atttcctgcc gttatatgac gttgactgcc agcaaaatgg caatctggaa
    64801 tatgacacct attcccaacc ggaatggaaa cataacttgt tcgaccatta tctggcggtg
    64861 ctttaccgct ttaaagatga aagtggcaaa gaacaattta gcggcgcagt ggtgaaaacg
    64921 cgtgaagcaa cgccgggtaa agaaatagag gcgatcactc gccgaatgct tgatttcagc
    64981 ccacgactga aaaaactcgt gggtgtgcca tgtcaggtct atgttcgcac cgtagcggcg
    65041 aataacgccc aaccgctaac ccaggatcaa tgcttacgcg cgctgcatca cttacgtgtt
    65101 caatccacca gcaaaactgc accacaggcg aaataatctc atctttgcta atgcgaggct
    65161 ggagccatcc agcctgaaat gactgataac gtgatcgttg gtcatgccgg atgcggcgta
    65221 aacgccttat ccggcctaca aattctcaag cattcaatca gttgcaatgc cttatgtagg
    65281 cctgatagcg aagcgcatct ggcaattata cgtttgtcac catcacatca gttcgggcac
    65341 ttatatacct ggccgataat ctggctatcc gtcgggacaa aactggacaa cattccctgc
    65401 gacgggctac tgatgccata aatcacgtta ccgcccattg cagccgcctg gttgcgcaga
    65461 tcgtttgctg cgccgcgcat agaaccgccc tcttctccgt gttgcccgga aagccagttg
    65521 ctttgcttac ctgtcgcagt accaatcagc tggcactctg cgcctggctg ctcgtccaca
    65581 atgcgtacac tctgaccggc agcactcaat tcgttgctgg agctacaacc cgccatcagt
    65641 agcgcggcac cgacaatccc tgctaagtat tttacgtgca tgttattccc cataatcaat
    65701 gagctggacg ccactcgcgt cgtccgtgtc tgaatcttat actagaaaga tgaccaaaag
    65761 aaaaaccccc agacatttct gcccgggggt ttgtttattt ctgcgaggtg cagggcaatt
    65821 acatcatgcc gcccatgcca cccatgccac ccatgccgcc agcagcgcct aagtcagctg
    65881 catcgttttt cggcaggtcg gtaaccatgc attcggtggt gatcatcagg ccagccacag
    65941 aagccgcgta ctgcagagca gaacgggtta ctttggttgg gtccaggata cccatgtcga
    66001 tcatgttgcc gtattcttcg gttgctgcgt tgtaaccgta gttgccgtcg ccgcctttaa
    66061 cggtgttagc aacaacagac ggttcttcgc cgcagttcag gacgatctga cgcagcggag
    66121 cttccattgc acgcagtgca actttgatac ccacgttctg gtcttcgttc tgaccacgca
    66181 ggtcagccag tttagacgct acgcggatca gcgcaacacc accaccagca accacgcctt
    66241 cttctaccgc agcacgggtc gcgtgcaggg cgtcttcaac gcgtgctttt ttctctttca
    66301 tttcaacttc ggtagcagca cctactttga taactgcaac gccgcctgcc agtttcgcta
    66361 cgcgctcctg cagtttttca cggtcgtagt cagaagttgc ttcttcaatc tgctgacgga
    66421 tctgagcaac acggccctgg attgcagctt cttcgcccac gccatcgatg atggtggtgg
    66481 tgtctttgtt gatcacaacg cgtttagcct gacccaggtc ttccagggtt gctttttcca
    66541 gctccatacc gatctcttca gagattacgg taccgccagt cagggttgcg atatcctgca
    66601 gcatagcttt acgacgatcg ccgaagcccg gagctttaac tgcagcaact ttcacgatgc
    66661 cacgcatggt gttaacaacc agagttgcca gcgcttcgcc ttctacatct tcagcgatga
    66721 tcagcagcgg tttgcctgct ttggcaacgg cttccagaac cggcagcatt tcgcggatgt
    66781 tggagatttt cttgtcagcc agcaggatga acgggctttc cagttctact gcgccagttt
    66841 ccggcttgtt gatgaagtaa ggagacaggt agccacggtc gaactgcata ccttcaacca
    66901 cgtccagttc gtcctgcaga ccggtaccgt cttcaacggt gataacgcct tctttaccga
    66961 ctttgtccat cgcttcagcg atcagtttac ctacggtttc gtcggagtta gcggagatag
    67021 taccaacctg agcaatcgct ttagagtcag agcacggtac ggacagcgct ttcagttctt
    67081 caactgcagc ggtaacagct ttgtcgatac cacgtttcag gtccatcggg ttcatgcccg
    67141 cagcaacagc tttcagacct tcagtgatga tagcctgagc cagtacggtt gcagtggtgg
    67201 taccgtcgcc tgcagcgtcg ttcgctttag aggcaacttc tttcaccatc tgcgcaccca
    67261 tgttttcgaa cttgtcttcc agttcgattt cacgagcaac ggaaacacca tctttggtga
    67321 tggtcggtgc accgaaagat ttatccagaa ctacgttacg gcctttcgga ccgagggtaa
    67381 ctttcactgc atctgccagt acgtttacgc cgcgcagcat tttcacacga gcgtcgttac
    67441 cgaattttac gtctttagct gccattatct ttattcctta aattcgtatg ttcagtgtcg
    67501 tgcgaggatt acgcttcaac aattgccaga atgtcgcttt cggacatgat caacacttct
    67561 tcattgtcga tcttctcgga tttcacaccg tagccatcgt tgaaaataac gatgtcgcca
    67621 actttcacat ccagcggctt cacttcgcca ttttcaagga tacggccatt gccgacagcc
    67681 agcacttcgc cgcgggtgga tttagccgct gcagagccgg tcagaacgat gccgccagca
    67741 gatttagttt caacttcttt acgcttgacg atcacgcgat catgcaatgg acgaatattc
    67801 attgataact ctcctttgag aaagtccgta tctgttatgg gtgacgccgg agcttacgtg
    67861 gtttcccggc tggtgaccag agaaatgggg atgaggcttc gccccttcaa gggggaaaaa
    67921 gaaaaaaaat tctgatcagc acaaaatcgg gtgaaaaccc tgattcacct cacatttcat
    67981 cgcaatttct tcatcgccgt cataagcgaa tctgattgtg ctaccatcga aaatctacgc
    68041 agttgcccaa aatttgggcg caatcggaca tcaacccatg agtggactca aacaagaact
    68101 ggggctggcc cagggcattg gcctgctatc gacgtcatta ttaggcactg gcgtgtttgc
    68161 cgttcctgcg ttagctgcac tggtagcggg caataacagc ctgtgggcgt ggcccatttt
    68221 gattatctta gtgttcccga ttgcgattgt gtttgcgatt ctgggtcgcc actatcccag
    68281 cgcaggcggc gtcgcgcact tcgtcggtat ggcgtttggt tcgcggcttg agcgagtcac
    68341 cggctggctg tttttatcgg tcattcccgt gggtttgcct gccgcgctac aaattgctgc
    68401 cggattcggc caggcaatgt ttggctggca tagctggcaa ctgttgttgg cagaactcgg
    68461 tacgctggcg ttggtgtggt atatcggtac tcgcggtgcc agttccagtg caaatctaca
    68521 aacagttatt gccggactta tcgtcgcact gattgtcgct atctggtggg cgggcgatat
    68581 caaacctgcg agtatcccct tccccgcgcc aggaaatatc gaacttaccg ggttatttgc
    68641 tgcgttatca gtgatgttct ggtgttttgt cggtctggaa gcatttgccc atcttgcctc
    68701 ggaatttaaa aatccagaac gtgattttcc tcgtgctttg atgattggtc tgctgctggc
    68761 aggattagtc tactggggct gtacggtagt cgtcttacac ttcgacgcct atggtgaaca
    68821 aatggccgcg gcagcatcgc ttccaaaaat tgtagtacaa ctgttcggtg taggagcgtt
    68881 atggattgcc tgcgtaattg gctatctggc ctgctttgcc agtctcaaca tttatataca
    68941 gagcttcgcc cgcctggtct ggtcgcaggc gcaacataat cctgaccact acctggcacg
    69001 cctctcttct cgccatatcc cgaataatgc cctcaatgcg gtgctcggct gctgcgtggt
    69061 gagcacgttg gtgattcatg ctttagagat caatctggac gctcttatta tttatgccaa
    69121 tggcatcttt attatgattt atctgttatg catgctggca ggctgtaaat tattgcaagg
    69181 tcgttatcga ctactggcgg tggttggcgg gctgttatgc gttctgttac tggcaatggt
    69241 cggctggaaa agtctctatg cgctgatcat gctggcgggg ttatggctgt ttctgccaaa
    69301 acgaaaaacg ccggaaaatg gcataaccac ataatccggc gtttcgacat taatcctggc
    69361 gatcgtcttt atgatcaagg cggtcgcggt catcatcctt tcgctggtac tcaccatcaa
    69421 aagtattacc gccaccggtc ccggcgctaa aaccgccgcc tggcatgcga gaaaagcgca
    69481 aatgcggcat caacttcact gtcagatgct tttgcaccgg cggcaataaa agcagaagac
    69541 cgaggaagtc ggtgaaaaag cccggtaata aaagcagcaa accggcaatg atcagcgaaa
    69601 cacttttaat catctccgcc gctgggtttt cacccgccgc cattttttgc tgcatcagca
    69661 cgaaattctt aaagccctgg ttacgtacca gtgacatacc gataactgac gtaaatataa
    69721 ccagcacgag agtcagcaat acccccaata catgggcaac ctgaataaag attgaaatct
    69781 caatatagac ataaaggaaa atggcaataa aaggtaacca gcgcaaaggt ttctcctgta
    69841 atagcagccg gttaaacccg gctacctgaa tgggttgcga atcgcgttca gcttatattg
    69901 tggtcattag caaaatttca agatatttgc gcaactattt ttggtagtaa tcccaaagcg
    69961 gtgatctatt tcacaaatta ataattaagg ggtaaaaacc gacacttaaa gtgatccaga
    70021 ttacggtaga aatcctcaag cagcatatga tctcggatat tcggtcgatg caggggataa
    70081 tcgtcggtcg aaaaacattc gaaaccacat atattctgtg tgtttaaagc aaatcattgg
    70141 cagcttgaaa aagaaggttc acatgtcaaa caacattcgt atcgaagaag atctgttggg
    70201 taccagggaa gttccagctg atgcctacta tggtgttcac actctgagag cgattgaaaa
    70261 cttctatatc agcaacaaca aaatcagtga tattcctgaa tttgttcgcg gtatggtaat
    70321 ggttaaaaaa gccgcagcta tggcaaacaa agagctgcaa accattccta aaagtgtagc
    70381 gaatgccatc attgccgcat gtgatgaagt cctgaacaac ggaaaatgca tggatcagtt
    70441 cccggtagac gtctaccagg gcggcgcagg tacttccgta aacatgaaca ccaacgaagt
    70501 gctggccaat atcggtctgg aactgatggg tcaccagaaa ggtgaatatc agtacctgaa
    70561 cccgaacgac catgttaaca aatgtcagtc cactaacgac gcctacccga ccggtttccg
    70621 tatcgcagtt tactcttccc tgattaagct ggtagatgcg attaaccaat tgcgtgaagg
    70681 ctttgaacgt aaagcggtcg aattccagga catcctgaaa atgggtcgta cccagctgca
    70741 ggacgcagta ccgatgaccc tcggtcagga attccgcgct ttcagcatcc tgctgaaaga
    70801 agaagtgaaa aacatccaac gtaccgctga actgctgctg gaagttaacc ttggcgcaac
    70861 agcaatcggt actggtctga acacgccgaa agagtactct ccgctggcag tgaaaaaact
    70921 ggctgaagtt actggcttcc catgcgtacc ggctgaagac ctgatcgaag cgacctctga
    70981 ctgcggcgct tatgttatgg ttcacggagc gctgaaacgc ctggctgtga agatgtccaa
    71041 aatctgtaac gacctgcgct tgctctcttc tggtccacgt gccggcctga acgagatcaa
    71101 cctgccggaa ctgcaggcgg gctcttccat catgccagct aaagtaaacc cggttgttcc
    71161 ggaagtggtt aaccaggtat gcttcaaagt catcggtaac gacaccactg ttaccatggc
    71221 agcagaagca ggtcagctgc agttgaacgt tatggagccg gtcattggcc aggctatgtt
    71281 cgaatccgtt cacattctga ccaacgcttg ctacaacctg ctggaaaaat gcattaacgg
    71341 catcactgct aacaaagaag tgtgcgaagg ttacgtttac aactctatcg gtatcgttac
    71401 ttacctgaac ccgttcatcg gtcaccacaa cggtgacatc gtgggtaaaa tctgtgccga
    71461 aaccggtaag agtgtacgtg aagtcgttct ggaacgcggt ctgttgactg aagcggaact
    71521 tgacgatatt ttctccgtac agaatctgat gcacccggct tacaaagcaa aacgctatac
    71581 tgatgaaagc gaacagtaat cgtacagggt agtacaaata aaaaaggcac gtcagatgac
    71641 gtgccttttt tcttgtgagc agtaacttaa aaataacaat ctaatatcaa cttgttaaaa
    71701 aacaaggaag gctaatatgc tagttgtaga actcatcata gttttgctgg cgatcttctt
    71761 gggcgccaga ttggggggaa taggtattgg ttttgcaggc ggattggggg tgctggttct
    71821 tgccgctatt ggcgttaaac ccggtaacat cccgttcgat gtcatttcca ttatcatggc
    71881 ggttatcgcc gctatttctg ccatgcaggt tgctggcggt ctggactatc tggttcatca
    71941 gacagaaaag ctgctgcgcc gtaacccgaa atacatcacg atcctcgcac cgatcgtgac
    72001 ctatttcctg actatctttg ctggtactgg caacatctct ctggcgacac tgccagttat
    72061 cgctgaagtt gcgaaggaac aaggcgttaa accttgccgt ccgctgtcta ctgcagtggt
    72121 atccgcgcag attgcgatca ccgcatcgcc aatctcagcg gcagtggttt acatgtcttc
    72181 cgtgatggaa ggtcatggca tcagctacct ccatctgctc tccgtggtca tcccgtccac
    72241 cctgctggcg gttctggtga tgtccttcct ggtcactatg ctgttcaact ccaaactctc
    72301 tgacgatccg atttatcgca agcgtctgga agagggcctg gttgaactgc gcggtgaaaa
    72361 gcagattgaa atcaaatccg gtgcaaaaac gtccgtctgg ctgttcctgc tgggcgtagt
    72421 tggcgtggtt atctatgcaa tcatcaacag cccaagcatg ggtctggttg aaaaaccgct
    72481 gatgaacacc accaacgcaa tcctgatcat catgctcagt gttgcaactc tgaccaccgt
    72541 tatctgtaaa gtcgataccg acaacatcct caactccagc accttcaaag caggtatgag
    72601 cgcctgtatt tgtatcctgg gtgttgcgtg gctgggcgat actttcgttt ccaacaacat
    72661 cgactggatc aaagataccg ctggtgaagt gattcagggt catccgtggc tgctggccgt
    72721 catcttcttc tttgcttctg ctctgctgta ctctcaggct gcaaccgcaa aagcactgat
    72781 gccgatggct ctggcactga acgtttctcc gctgaccgct gttgcttctt tcgctgcggt
    72841 gtctggtctg ttcattctgc cgacctaccc gacgctggtt gctgcggtac agatggatga
    72901 cacgggtact acccgtatcg gtaaattcgt cttcaaccat ccgttcttca tcccgggtac
    72961 tctgggtgtt gccctggccg tttgcttcgg cttcgtgctg ggtagcttca tgctgtaatg
    73021 accaatcgcg gggcgttcac gccccgcttt ctttctcgcc gactaacatc ctttccccgt
    73081 ccgttgtata gtgacctctc tcttgcggtt ccatctgttc ttgcgaggtg tttatgcttg
    73141 atgaaaaaag ttcgaatacc gcgtctgtcg tggtgctatg taccgcaccg gatgaagcga
    73201 cagcccagga tttagccgcc aaagtgctgg cggaaaaact ggcggcctgc gcgaccttga
    73261 tccccggcgc tacctctctc tattactggg aaggtaagct ggagcaagaa tacgaagtgc
    73321 agatgatttt aaaaactacc gtatctcacc agcaggcact gctggaatgc ctgaagtctc
    73381 atcatccata tcaaaccccg gaacttctgg ttttacctgt tacacacgga gacacagatt
    73441 acctctcatg gctcaacgca tctttacgct gatcctgcta ctttgcagca cttccgtttt
    73501 tgccggatta ttcgacgcgc cgggacgttc acaatttgtc cccgtggatc aagcctttgc
    73561 ttttgatttt cagcaaaacc aacatgacct taatctgacc tggcagatca aagacggtta
    73621 ctacctctac cgtaaacaga tccgcattac gccggaacac gcgaaaattg ccgacgtgca
    73681 gctgccgcaa ggcgtctggc atgaagatga gttttacggc aaaagcgaga tttaccgcga
    73741 tcggctgacg cttcccgtaa ccatcaacca ggcgagtgcg ggagcgacgt taactgtcac
    73801 ctaccagggc tgtgctgatg ccggtttctg ttatccgcca gaaaccaaaa ccgttccgtt
    73861 aagcgaagtg gtcgccaaca acgcagcgtc acagcctgtg tctgttccgc agcaagagca
    73921 gcccaccgcg caattgccct tttccgcgct ctgggcgttg ttgatcggta ttggtatcgc
    73981 ctttacgcca tgcgtgctgc caatgtaccc actgatttct ggcatcgtgc tgggtggtaa
    74041 acagcggctc tccactgcca gagcattgtt gctgaccttt atttatgtgc aggggatggc
    74101 actgacttac acggcgctgg gtctggtagt tgccgccgca gggttacagt tccaggcggc
    74161 gctacagcac ccatacgtgc tcattggcct cgccatcgtc tttaccttgc tggcgatgtc
    74221 aatgtttggc ttgcttactc tacaactccc ctcctcgctg caaacgcgcc tcacgctgat
    74281 gagcaatcgc caacagggcg gctcacccgg cggagtgttt attatgggga cgattgccgg
    74341 actgatctgt tcaccatgca ccaccgcacc gcttagcgcg attctgctgt atatcgccca
    74401 aagcgggaac atgtggctgg gcggcggcac gctttatctc tatgcgttgg gcatgggcct
    74461 gccgctgatg ctaattaccg tctttggtaa ccgcttgctg ccgaaaagcg gcccgtggat
    74521 ggaacaagtc aaaaccgcgt ttggttttgt gatcctcgca ctgccggtct tcctgctgga
    74581 gcgagtgatt ggtgatgtat ggggattacg cttgtggtcg gcgctgggtg tcgcattctt
    74641 tggctgggcc tttatcacca gcctacaggc caaacgcggc tggatgcgcg tggtgcaaat
    74701 aatcctgctt gcagcggcat tggttagcgt gcgcccactt caggattggg catttggtgc
    74761 gacgcatacc gcgcaaactc agacgcatct caactttaca caaatcaaaa cggtagatga
    74821 gttaaatcag gcgctcgttg aagccaaagg caaaccggtg atgttagatc tttatgccga
    74881 ctggtgcgtc gcctgtaaag agtttgagaa atacaccttc agcgacccgc aggtgcaaaa
    74941 agcgttagca gacacggtat tacttcaggc caacgtcact gccaacgacg cacaagatgt
    75001 ggcgctgtta aagcatctta atgtccttgg cctaccgaca attctctttt ttgacggaca
    75061 aggccaggag catccacaag cacgcgtcac gggctttatg gatgctgaaa ccttcagcgc
    75121 acatttgcgc gatcgccaac cgtgaacgac acttgcagtg ggaaagacgg aggaaaatag
    75181 cgtgcaacgt gaagatgtac tgggagaagc cctgaaatta ttagaattac aagggattgc
    75241 caacaccacg ctggagatgg ttgctgaacg tgtggattat ccactggacg agctacgccg
    75301 cttctggcca gacaaagagg caatcctcta cgatgcgctg cgttatctta gccaacagat
    75361 agatgtctgg cgtcgtcagc tgatgctgga cgaaacgcaa accgccgaac aaaagctgct
    75421 ggcacgttat caggcgttat cggagtgcgt taaaaacaac cgctatccgg gctgtctgtt
    75481 tatcgctgcc tgtacgtttt atcccgatcc tggccaccct attcatcaac tggccgatca
    75541 gcaaaaaagc gcggcctacg atttcaccca cgaactgtta accacgctgg aagttgacga
    75601 tccggcgatg gtagcaaagc agatggaact ggtgctggaa ggctgtttaa gccgaatgct
    75661 ggtgaatcgt agccaggcgg atgtcgacac cgcacatcgg ctggcggaag atatcctgcg
    75721 cttcgcccgc tgccgccagg gtggtgcact gacctgacag aaacacagaa aagaagcgat
    75781 ttgccgcaat cttaagcagt tgaatcgctt ttactgaaat taggttgacg agatgtgcag
    75841 attacggttt aatgcgcccc gttgcccgga tagctcagtc ggtagagcag gggattgaaa
    75901 atccccgtgt ccttggttca attccgagtc cgggcaccaa attcatataa acggacctcc
    75961 acggaggtcc gtttttcgtt tcaggacgcc acgatttaag tgttctgcct ccaaatcaat
    76021 tctaccgaac tcaacccgat tctccccaca tcaccagcaa tttgcgggca tatcccaatt
    76081 cgggaaaatt tgttcctgag ctatagcact aactaacgtg aaatgtcgtg cggccccgtg
    76141 atgctgttga acgtcaaatg acgtcatcag gagcgtaacg catccataaa gcgcaacatc
    76201 ggtctgaacg ccgactgatg agattttctg aatgagaaca aagagaaatg tatcagtccg
    76261 cttgctcatg caaagactaa caatccatta aaatagtaag cgctccggac aattttccat
    76321 ggattatttt ctgaacattt ttctttggca aagatgatga attttgatgg taaggaatat
    76381 tacttctggt tctcagtaaa atcctttcgt aataccatgt aatcaagaag tttatggctg
    76441 gtaaaaataa cgtcttgcat tcaccaataa tatgtaaata aacccatcta tagatggtaa
    76501 aaataggttg tggcaattat cattgcatca ttcccttttc gaatgagttt ctattatgca
    76561 acaacctgta gttcgcgttg gcgaatggct tgttactccg tccataaacc aaattagccg
    76621 caatgggcgt caacttaccc ttgagccgag attaatcgac cttctggttt tctttgctca
    76681 acacagtggc gaagtactta gcagggatga acttatcgat aatgtctgga agagaagtat
    76741 tgtcaccaat cacgttgtga cgcagagtat ctcagaacta cgtaagtcat taaaagataa
    76801 tgatgaagat agtcctgtct atatcgctac tgtaccaaag cgcggctata aattaatggt
    76861 gccggttatc tggtacagcg aagaagaggg agaggaaata atgctatctt cgcctccccc
    76921 tataccagag gcggttcctg ccacagattc tccctcccac agtcttaaca ttcaaaacac
    76981 cgcaacgcca cctgaacaat ccccagttaa aagcaaacga ttcactacct tttgggtatg
    77041 gttttttttc ctgttgtcct taggtatctg tgtagcactg gtagcgtttt caagtcttga
    77101 tacacgtctt ccgatgagca aatcgcgtat tttgctcaat ccacgcgata ttgacattaa
    77161 tatggtaaat aaaagttgta acagctggag ttccccgtat cagctctctt acgcgatagg
    77221 cgtgggtgat ttggtggcga catcacttaa caccttctcc acctttatgg tgcatgacaa
    77281 aatcaactac aacattgatg aaccgagcag ttccggtaaa acattatcta ttgcgtttgt
    77341 taatcagcgc caataccgtg ctcaacaatg ctttatgtcg ataaaattgg tagacaatgc
    77401 agatggttca accatgctgg ataaacgtta tgtcatcact aacggtaatc agctggcgat
    77461 tcaaaatgat ttactggaga gtttatcaaa agcgttaaac caaccgtggc cacaacgaat
    77521 gcaggagacg ctccagcaaa ttttgcctca tcgtggtgcg ttattaacta atttttatca
    77581 ggcacatgat tatttactgc atggcgatga taaatcattg aaccgtgcca gtgaattatt
    77641 aggtgagatt gttcaatcat ccccagaatt tacctacgcg agagcagaaa aagcattagt
    77701 tgatatcgtg cgccattctc aacatccttt agatgaaaaa caattagcag cactgaacac
    77761 agaaatagat aacattgtta cactaccgga attgaataac ctgtccatta tatatcaaat
    77821 aaaagcggtc agtgctctgg taaaaggtaa aacagatgag tcttaccagg cgataaatac
    77881 tggcattgat cttgaaatgt cctggctaaa ttatgtattg cttggcaagg tttatgaaat
    77941 gaaggggatg aaccgggaag cggctgatgc atatctcacc gcctttaatt tacgcccagg
    78001 ggcaaacacc ctttactgga ttgaaaatgg tatattccag acttctgttc cttatgttgt
    78061 accttatctc gacaaatttc tcgcttcaga ataagtaacc cccgggttga ttcatgctcg
    78121 ggaatatttg ttgttgagtt tttgtatgtt cctgttggta taatatgttg cggcaattta
    78181 tttgccgcat aatttttatt acataaattt aaccagaaga cgtcacgcaa tctattgtaa
    78241 acattgaatg tttatctttt catgatatca acttgcgatc ctgatgtgtt aataaaaaac
    78301 ctcaagttct cacttacaga aacttttgtg ttatttcacc taatctttag gattaatcct
    78361 tttttcgtga gtaatcttag cgccagtttg gtctggtcag gaaatagtta tacatcatga
    78421 cccggactcc aaattcaaaa atgaaattag gagaagagca tgagttctgc caagaagatc
    78481 gggctatttg cctgtaccgg tgttgttgcc ggtaatatga tggggagcgg tattgcatta
    78541 ttacctgcga acctagcaag tatcggtggt attgctatct ggggttggat tatctctatt
    78601 attggtgcaa tgtcgctggc gtatgtatat gcccgactgg caacaaaaaa cccgcaacaa
    78661 ggtggcccaa ttgcttatgc cggagaaatt tctcctgcat ttggttttca gacaggtgtt
    78721 ctttattacc atgctaactg gattggtaac ctggcgattg gtattaccgc tgtatcttat
    78781 ctttccacct tcttcccagt attaaatgat cctgttccgg cgggtatcgc ctgtattgct
    78841 atcgtctggg tatttacctt tgtaaatatg ctcggcggta cctgggtaag ccgtttaacc
    78901 actattggtc tggtgctggt tcttattcct gtggtgatga ctgctattgt tggctggcat
    78961 tggtttgatg cggcaactta tgcagctaac tggaatactg cggataccac tgatggtcat
    79021 gcgatcatta aaagtattct gctctgcctg tgggccttcg tgggtgttga atccgcagct
    79081 gtaagtactg gtatggttaa aaacccgaaa cgtaccgttc cgctggcaac catgctgggt
    79141 actggtttag caggtattgt ttacatcgct gcgactcagg tgctttccgg tatgtatccg
    79201 tcttctgtaa tggcggcttc cggtgctccg tttgcaatca gtgcttcaac tatcctcggt
    79261 aactgggctg cgccgctggt ttctgcattc accgcctttg cgtgcctgac ttctctgggc
    79321 tcctggatga tgttggtagg ccaggcaggt gtacgtgccg ctaacgacgg taacttcccg
    79381 aaagtttatg gtgaagtcga cagcaacggt attccgaaaa aaggtctgct gctggctgca
    79441 gtgaaaatga ctgccctgat gatcctcatc actctgatga actctgccgg tggtaaagca
    79501 tctgacctgt tcggtgaact gaccggtatc gcagtactgc tgactatgct gccgtacttc
    79561 tactcttgcg ttgacctgat tcgttttgaa ggcgttaaca tccgcaactt tgtcagcctg
    79621 atctgctctg tactgggttg cgtgttctgc ttcatcgcgc tgatgggcgc aagctccttc
    79681 gagctggcag gtaccttcat cgtcagcctg attatcctga tgttctacgc tcgcaaaatg
    79741 cacgagcgcc agagccactc aatggataac cacaccgcgt ctaacgcaca ttaattaaaa
    79801 gtattttccg aggctcctcc tttcattttg tcccatgtgt tgggaggggc cttttttacc
    79861 tggagatatg actatgaacg ttattgcaat attgaatcac atgggggttt attttaaaga
    79921 agaacccatc cgtgaacttc atcgcgcgct tgaacgtctg aacttccaga ttgtttaccc
    79981 gaacgaccgt gacgacttat taaaactgat cgaaaacaat gcgcgtctgt gcggcgttat
    80041 ttttgactgg gataaatata atctcgagct gtgcgaagaa attagcaaaa tgaacgagaa
    80101 cctgccgttg tacgcgttcg ctaatacgta ttccactctc gatgtaagcc tgaatgacct
    80161 gcgtttacag attagcttct ttgaatatgc gctgggtgct gctgaagata ttgctaacaa
    80221 gatcaagcag accactgacg aatatatcaa cactattctg cctccgctga ctaaagcact
    80281 gtttaaatat gttcgtgaag gtaaatatac tttctgtact cctggtcaca tgggtggtac
    80341 tgcattccag aaaagcccgg taggtagcct gttctatgat ttctttggtc cgaataccat
    80401 gaaatctgat atttccattt cagtatctga actgggttct ctgctggatc acagtggtcc
    80461 acacaaagaa gcagaacagt atatcgctcg cgtctttaac gcagaccgca gctacatggt
    80521 gaccaacggt acttccactg cgaacaaaat tgttggtatg tactctgctc cggcaggcag
    80581 caccattctg attgaccgta actgccacaa atcgctgacc cacctgatga tgatgagcga
    80641 tgttacgcca atctatttcc gcccgacccg taacgcttac ggtattcttg gtggtatccc
    80701 acagagtgaa ttccagcacg ctaccattgc taagcgcgtg aaagaaacac caaacgcaac
    80761 ctggccggta catgctgtaa ttaccaactc tacctatgat ggtctgctgt acaacaccga
    80821 cttcatcaag aaaacactgg atgtgaaatc catccacttt gactccgcgt gggtgcctta
    80881 caccaacttc tcaccgattt acgaaggtaa atgcggtatg agcggtggcc gtgtagaagg
    80941 gaaagtgatt tacgaaaccc agtccactca caaactgctg gcggcgttct ctcaggcttc
    81001 catgatccac gttaaaggtg acgtaaacga agaaaccttt aacgaagcct acatgatgca
    81061 caccaccact tctccgcact acggtatcgt ggcgtccact gaaaccgctg cggcgatgat
    81121 gaaaggcaat gcaggtaagc gtctgatcaa cggttccatt gaacgtgcga tcaaattccg
    81181 taaagagatc aaacgtctga gaacggaatc tgatggctgg ttctttgatg tatggcagcc
    81241 ggatcatatc gatacgactg aatgctggcc gctgcgttct gacagcacct ggcacggctt
    81301 caaaaacatc gataacgagc acatgtatct tgacccgatc aaagtcaccc tgctgactcc
    81361 ggggatggaa aaagacggca ccatgagcga ctttggtatt ccggccagca tcgtggcgaa
    81421 atacctcgac gaacatggca tcgttgttga gaaaaccggt ccgtataacc tgctgttcct
    81481 gttcagcatc ggtatcgata agaccaaagc actgagcctg ctgcgtgctc tgactgactt
    81541 caaacgtgcg ttcgacctga acctgcgtgt gaaaaacatg ctgccgtctc tgtatcgtga
    81601 agatcctgaa ttctatgaaa acatgcgtat tcaggaactg gctcagaata tccacaaact
    81661 gattgttcac cacaatctgc cggatctgat gtatcgcgca tttgaagtgc tgccgacgat
    81721 ggtaatgact ccgtatgctg cattccagaa agagctgcac ggtatgaccg aagaagttta
    81781 cctcgacgaa atggtcggtc gtattaacgc caatatgatc cttccgtatc cgccgggagt
    81841 tcctctggta atgccgggtg aaatgatcac cgaagaaagc cgtccggttc tggagttcct
    81901 gcagatgctg tgtgaaatcg gcgctcacta tccgggcttt gaaaccgata ttcacggtgc
    81961 ataccgtcag gctgatggcc gctataccgt taaggtattg aaagaagaaa gcaaaaaata
    82021 attagctcgt acaagggaag tggcttgcca cttccctttt ttgctcataa ggagaacaca
    82081 tgaaaacacc ctcacagccg cgcgcgatat actatatcgt ggcgatccaa atctgggagt
    82141 acttcagttt ttacggcatg cgtgccttac tcattctcta tctcacccat cagcttggtt
    82201 ttgatgataa ccatgccatc agcctgttca gcgcatatgc ttctctggtt tacgttaccc
    82261 ctattctcgg cggctggctt gccgaccgcc tgctcggcaa ccgcactgca gtgattgccg
    82321 gcgcgctgtt aatgaccctt ggccatgtgg tgctgggtat tgatacaaat tcaaccttta
    82381 gcctgtatct ggcgctggca atcattattt gtggctacgg tttattcaaa tcaaacatca
    82441 gctgtttgct tggcgagctc tacgacgaga acgatcatcg acgtgatggc ggtttttcgc
    82501 tgctgtatgc tgcgggcaat atcggttcta tcgccgcccc aatcgcctgc ggcctggctg
    82561 ctcagtggta tggatggcat gttggctttg cccttgcggg tggcggcatg tttatcggtt
    82621 tgttgatttt cttaagcggg catcgtcatt tccagtccac gcgtagtatg gataaaaaag
    82681 cgctcactag cgtcaaattt gccttaccgg tatggagctg gttagtggtg atgctctgtt
    82741 tagccccagt attttttacc ctgctgctgg agaacgactg gtccggatat ttgctggcga
    82801 tcgtttgcct cattgccgca caaatcattg cccgcatgat gatcaaattc cccgaacacc
    82861 gccgtgctct ttggcaaatt gtattgttga tgtttgtcgg gacattgttc tgggtactgg
    82921 cacaacaggg cggcagtacc atcagcttgt ttatcgatcg ctttgtgaat cgtcaggcat
    82981 tcaatattga agtacctact gcactattcc agtcggtgaa tgcaattgcg gtgatgctcg
    83041 ctggggttgt tctggcctgg ctggcgtcgc cagaaagcca cggcaactca acattgcgcg
    83101 tctggctgaa gtttgccttt ggcttactgc tgatggcttg tggctttatg ttgctggcat
    83161 ttgatgcccg acatgcagca gctgacggtc aagcgtcaat gggcgtgatg gtatccgggc
    83221 tggcgctaat gggctttgcc gaactcttta ttgacccggt ggcgattgcg caaatcacgc
    83281 gtctgaaaat gtctggcgta ttaaccggga tttatatgct ggcaacaggc gcggtcgcca
    83341 actggctggc aggcgtagtg gcacagcaga cgacagagtc gcaaattagc ggtatggcaa
    83401 ttgcagctta ccagcgattc ttttctcaga tgggagagtg gacgttggct tgtgtcgcga
    83461 tcatcgtggt attggccttt gctacccgtt tcctgtttag cacgccgacg aatatggtac
    83521 aggagagcaa cgattaactg tcgtaaaaac aattggttat gaataaatta actttcagtt
    83581 tatcacacta gtaaatcgag attaataagt catttaaacc attttgatgg ttatttatta
    83641 gtgatatcaa cttgaggtaa gcgttagttt cgataagata aactgagtta ttaatagtcg
    83701 aggcagataa tacagtgtac cgatctgtct ctttatctac gctaaattga aagctggatt
    83761 tagaggaacc aaaatgtctg aacaagaaac acggggagcc aatgaggcta ttgattttaa
    83821 cgatgaactg agaaatcgcc gcgaaaaact ggcggcacta cgtcagcaag gtgtggcgtt
    83881 tcccaatgat tttcgccgcg accatacctc tgaccagttg cacgaagagt ttgatgcgaa
    83941 ggataaccag gaactggaat ccttaaacat tgaagtctcg gttgctggcc gaatgatgac
    84001 ccgtcgtatc atggggaaag cctcctttgt aacgttgcag gatgtcggtg gccgtattca
    84061 actgtacgtc gcacgagata gcctgccaga aggtgtttat aacgatcagt ttaaaaaatg
    84121 ggatctgggt gacattatcg gtgcccgcgg tacgctgttt aagacgcaaa cgggtgagct
    84181 ttccattcac tgtactgagt tgcgcctgct gactaaagca ctacgtcctt taccagataa
    84241 attccatggt ctgcaggatc aggaagtccg ttatcgtcaa cgttatctgg acctcatcgc
    84301 taacgataaa tcccgtcaaa cgtttgttgt ccgttcaaaa attctggccg ctatccgcca
    84361 gttcatggtc gcgcgcggct ttatggaagt agaaaccccg atgatgcagg taattccagg
    84421 tggggcatct gctcgcccgt ttattaccca tcataatgct ctggatttag atatgtatct
    84481 gcgtatcgcg ccggagctgt atctgaaacg tctggttgta ggcggttttg aacgggtatt
    84541 cgaaatcaac cgtaacttcc gtaatgaagg tatttctgtt cgccataatc ctgagttcac
    84601 aatgatggaa ctctacatgg cgtatgcgga ttaccacgat ttgattgaac tgacagagtc
    84661 cctgttccgc accctggcac aagaggttct gggtaccact aaagtcactt atggcgagca
    84721 tgtgtttgat ttcggcaaac cgtttgaaaa actcaccatg cgcgaagcaa tcaaaaaata
    84781 tcgtccagaa accgatatgg ccgacctgga taattttgat gctgctaaag cattagctga
    84841 atctatcggt attacggtag agaaaagctg ggggctggga cgtattgtca cggagatctt
    84901 tgatgaagtg gcagaagcac atctgattca gccaaccttt attacggaat atccggcaga
    84961 agtgtcaccg ctggcacgcc gtaatgatgt taacccggaa atcaccgacc gttttgaatt
    85021 cttcatcggt ggtcgtgaaa tcggtaatgg ttttagcgaa ttaaacgacg cagaagatca
    85081 ggctgaacgt ttccaggaac aggttaacgc taaagccgca ggtgacgacg aagccatgtt
    85141 ctatgacgaa gattacgtga ctgcgctgga atatggtctg ccgccaaccg ctggtctggg
    85201 tattggtatc gaccgaatga ttatgctgtt tactaacagc catactattc gcgacgttat
    85261 tctcttcccg gcgatgcgcc cacagaaata aatttcactt taatgaacga agcagtcagg
    85321 cgactgcttc gttcatcgtt ccgcaaatcc agttttgagg agagacgctt cgtttcacga
    85381 taaccagcgg gtccactcaa ctacagctcc taaaagagag aaaaaagtaa tgccacaggc
    85441 agactcattg gccaggttat tccgaccagg aatgcactaa gtaaacgaat atgtgttttc
    85501 tcatgtgaga taaaccagat aatgacaaag gatatgttca caccaatcac ataaaaaatt
    85561 aatattttag agaatagtgc cattttttat gttcttctta tccttactgg ctacttgtaa
    85621 aactgacgga aatagtcatc aactgtcata atgttgaggt caaagtcaat atccggtaag
    85681 tctaagcatt taagcctgat taaatcttcg atttcacttg cggctaacga tgtcgttatt
    85741 gcgaaggaat tttcattcaa ttgtcgtggt atcccatatt catcagccac gatagaagag
    85801 aaattcatat catacatcaa tgatcttaac cttaagaaca ctgaggaata agatgatgga
    85861 taatcaaaag aaacaacata agtattgaac ttgtttttac cttccataat atgactccta
    85921 taaatattga acactctggg gacgcaaaat ttattaaccc cgattgatga ttctcatata
    85981 ggagatattc ttttttttgc tcagtaaagg ggatgacttg ggacaaaaaa acgcgctctc
    86041 tgcccattct ttgtgcatcc ccctaccctt cagatgctct cctgcatcac accgcggaga
    86101 gcatgaaaaa tatcgtgaat ctgtactctt ccatcaactg cgaatatcat catactcccg
    86161 cgttttatca aattcgtgtt tcgcaaatgg acataatggg ataatttttc gtttttcccg
    86221 acgcattttt tccacgactt tcgcaaccag ctgtttacca atcccttgcc ctttcaggct
    86281 ttcatcgaca tcggtatgtt cgataatcgc taaattctct ccggtcggca caaagacaat
    86341 ttcagcgatt tgcttgcctt gttcgtcatt aatgtaaaat ttattgtggc cttcgcgtat
    86401 ttccatttta ccctcgctta tttatgacgg tatttcagcg caccactcgg gcacgtatca
    86461 atcactttaa ccacagtggc aacgtcgact tcatccggca tgatccacgg ctttcgtttg
    86521 agattaaata acttgccgtt gccacgtacg caattgccag aatgctgaca tatcgcagtg
    86581 ttgaaataga catcgatctt ttcgccggta taacagcgat aacccccgtc cagtagcgcc
    86641 tgatccatga ccttccctct ctcatcagta tggttttcac tataagcata gccctggaat
    86701 cttcatacag cactggcagt ttccgttgcc agagtctctt cccctatata gccaatcatt
    86761 cttactccct ttgaattacc cgcctcatca gagataatgc ttaagaaatt tgtcacacaa
    86821 ggaagctgat gagacattca ttgccctacc gcatgttacg caaacgtccg atgaaattga
    86881 gtaccacagt gatcttaatg gtcagcgcgg tactgttctc ggtgctattg gtggtgcatc
    86941 tgatttactt ctcgcaaatc agtgatatga cgcgagacgg gctagccaac aaggcactgg
    87001 cagtggcgcg tactctcgcc gactcgccgg aaatccgtca gggcttgcag aaaaaaccgc
    87061 aggagagcgg catccaggcc atcgcggaag ccgtgcgcaa acgcaacgat ctgctgttta
    87121 ttgtcgttac cgatatgcaa agtcttcgct actcgcatcc tgaagcccag cgtattggtc
    87181 agccatttaa aggtgatgac atccttaaag cgctgaatgg cgaagaaaat gtcgctatca
    87241 atcgcggttt tctggcgcag gctttacgcg tatttacccc catctacgat gaaaatcata
    87301 aacaaattgg cgtggtggcg atcggccttg agttaagccg cgtgacccaa cagatcaatg
    87361 acagtcgctg gagcattatc tggtcggtat tatttggcat gctggtcgga ctgattggca
    87421 cctgcattct ggttaaggta ctgaaaaaaa tccttttcgg cctggaaccc tacgaaatct
    87481 ccacgctgtt tgagcaacgc caggccatgt tgcagtctat caaagaaggc gtcgttgccg
    87541 tggacgatcg cggcgaggtc acgctgatca acgatgccgc acaagaattg ctgaattacc
    87601 gtaagtcgca ggacgatgag aaactgtcga cgctcagcca ctcatggtca caggtggtag
    87661 atgtctcgga agtgttacgc gacggtacgc cgcgccgcga cgaagagatt acgattaaag
    87721 accggctatt actgatcaac accgttccgg tgcgcagtaa tggcgttatc atcggtgcca
    87781 tttcaacctt cagggacaaa actgaagttc gtaaactgat gcagcgactc gacggtctgg
    87841 tcaactatgc tgatgcactt cgtgaacgat cccacgaatt tatgaataaa ttgcatgtga
    87901 ttctcggatt attgcatctg aagagttata agcagttgga agattacatt ctcaaaacag
    87961 ccaataacta tcaggaagag attggctctc tgctgggtaa gatcaaatct ccggttatcg
    88021 ctggtttttt aatcagcaag attaaccgcg cgaccgattt aggccatacg ctgattttaa
    88081 acagtgaaag ccagctgcca gacagcggca gtgaggacca ggtcgcgacg ctgattacca
    88141 cattgggaaa tctgatagaa aacgcgctgg aagcattagg gccggaaccc ggtggcgaaa
    88201 ttagcgtaac attgcactac cgtcacggct ggctgcactg cgaagttaac gatgatggac
    88261 cggggatcgc acccgacaaa atcgatcaca tttttgacaa aggtgtctcg acaaaaggaa
    88321 gcgagcgagg cgtcggttta gcacttgtca aacaacaggt agaaaatctc ggcggcagca
    88381 tcgccgtgga atcggaaccc gggattttca cacaattttt tgtccagata ccctgggacg
    88441 gggagaggtc gaacagatga tcaatgtatt aattatcgat gacgacgcaa tggtcgcgga
    88501 gctgaatcgc cgatacgtag cacaaatccc aggctttcaa tgctgtggaa cagcctcgac
    88561 gctggagaaa gccaaagaga ttatcttcaa tagcgatacg cctatcgacc tgatattgct
    88621 cgatatctat atgcaaaaag agaacgggct cgatttactg cctgtcctgc ataacgcgcg
    88681 ttgcaaaagt gatgtgattg tcatctcctc cgcagccgat gcggcaacca ttaaagattc
    88741 gctgcattac ggtgtcgtgg attacctgat caaacccttc caggcttccc gctttgaaga
    88801 ggcgctcacc ggctggcggc aaaagaaaat ggcgctggaa aaacatcagt attatgacca
    88861 ggccgagctt gatcagctaa tccacggcag cagctccaac gagcaggatc ctcgtcgctt
    88921 gccaaaaggc ttaacgccgc agacgttacg cacactgtgc cagtggattg acgcgcatca
    88981 ggactatgag ttttcaaccg acgagctagc caacgaggtt aacatttcgc gtgtttcctg
    89041 ccgtaaatac cttatctggc tggtcaactg ccacatcttg ttcaccagta tccattatgg
    89101 cgtcacgggg cgtccagttt accgttaccg tattcaggca gaacactact cactactgaa
    89161 acaatattgc caataacgga tcgctggtta tctgtaagta ataattatta ccgtaatgca
    89221 aaatacccct ggtacaagtt acatccttca tcagtaaaac ttgtgccaga tcaaatataa
    89281 ttatccctcc atcatcgcta aaaattaata tctcttcagg tgaacggtgt ttttaatttc
    89341 aaaacgctaa caaaagttaa ttaactatta tgtcacccgc attatgtgta tttttaccca
    89401 caaatgggta gatcagatta atctataaac ctaatgatat ctgccctgag aacaaaaaat
    89461 agaccgataa atatcaataa gataacagca aacaaaacat taacatctgc gcagtacaaa
    89521 ctttaaaccc atcgccagag agtttttctc tctgaaaaag ccgcttatca cagtgcataa
    89581 atttgccgct gctttaatca gccaatattc actgtgaggt atttgctaaa gccggtaacg
    89641 accaaacgga tatttagtca ggctctgaaa acagttcata caaaacagaa cgtgactgtg
    89701 atctattcag caaaaattta aataggatta tcgcgagggt tcacacatgt tatttactat
    89761 ccaacttatc ataatactga tatgtctgtt ttatggtgcc agaaagggtg gtatcgcgct
    89821 gggtttatta ggcggtatcg gtctggtcat tctggtcttc gtcttccacc ttcagccagg
    89881 taaaccacca gttgatgtca tgctggttat cattgcggtg gtggcggcat cggcgacctt
    89941 gcaagcttcg ggcggtcttg atgtcatgct gcaaattgcc gagaagctgc tgcgccgcaa
    90001 cccgaaatat gtctcaattg tcgcgccgtt tgtgacctgt acgctgacca ttctttgcgg
    90061 tacgggtcat gtggtttaca ccattctgcc gatcatctac gacgtcgcca ttaagaacaa
    90121 catccgtccg gaacgtccga tggcggcaag ttctatcggt gcacagatgg ggattatcgc
    90181 cagtccggtg tcggttgcgg tcgtgtctct ggttgcaatg ctgggtaatg tcacctttga
    90241 tggtcgccat cttgagttcc tcgacctgct ggcaatcacc attccatcga cgttaatcgg
    90301 tatcctggcg atcggtatct tcagctggtt ccgcggtaaa gatctggata aagacgaaga
    90361 gttccagaaa ttcatctccg taccggaaaa ccgtgagtat gtttacggtg ataccgcgac
    90421 gctgctggat aaaaaactgc cgaaaagcaa ctggctggca atgtggattt tcctcggggc
    90481 aatcgctgta gtcgcacttc ttggtgctga ttcggacctg cgtccatcct tcggcggcaa
    90541 accgctgtcg atggtactgg ttattcagat gtttatgctg ctgaccgggg cgctgattat
    90601 tatcctgacc aaaaccaatc ccgcgtctat ctcaaaaaac gaagtcttcc gttccggtat
    90661 gatcgccatc gtggcggtgt acggtatcgc atggatggca gaaaccatgt tcggtgcgca
    90721 tatgtctgaa attcagggcg tactgggtga aatggtgaaa gagtatccgt gggcctatgc
    90781 cattgttctg ctgctggttt ccaagtttgt aaactctcag gctgcggcgc tggcggcgat
    90841 tgttccggtc gcgctagcga tcggcgttga tccggcatac atcgtggctt cagcaccggc
    90901 ttgctacggt tattacatcc tgccgactta tccgagcgat ctggcagcga ttcagtttga
    90961 ccgttccggc accacccaca tcggtcgctt cgtcatcaac cacagcttta ttctgccggg
    91021 gttgattggt gtgagcgtat cgtgcgtctt cggctggatc ttcgccgcga tgtacgggtt
    91081 cttataaatg cactttgcgt gccgcccgtt actacgcggc acgccatttt cgaataacaa
    91141 atacagagtt acaggctgga agctatgtca aacaaaccct ttatctacca ggcacctttc
    91201 ccgatgggga aagacaatac cgaatactat ctactcactt ccgattacgt tagcgttgcc
    91261 gacttcgacg gcgaaaccat cctgaaagtg gaaccagaag ccctgaccct gctggcgcag
    91321 caagccttcc acgacgcttc tttcatgctc cgtccggcac accagaaaca ggttgcggct
    91381 attcttcacg atccagaagc cagcgaaaac gacaagtacg tggcactgca attcttaaga
    91441 aactccgaaa tcgccgccaa aggcgtgctg ccgacctgcc aggataccgg caccgcgatc
    91501 atcgtcggta aaaaaggcca gcgcgtgtgg accggtggcg gtgatgaaga agcgctgtcg
    91561 aaaggcgtct ataacaccta tatcgaagat aacctgcgct actcgcaaaa cgcggcgctg
    91621 gacatgtaca aagaggtcaa caccggcact aacctgcctg cgcaaatcga cctgtacgcg
    91681 gtagatggcg atgagtacaa attcctttgc gttgcgaaag gcggcggctc tgccaacaaa
    91741 acctatctct accaggaaac caaagccctg ctgactcccg gcaaactgaa aaacttcctc
    91801 gtcgagaaaa tgcgtaccct cggtactgca gcctgcccgc cgtaccatat cgcgtttgtg
    91861 attggcggta cgtctgtgga aaccaacctg aaaaccgtta agttagcaag cgctcactat
    91921 tacgatgaac tgccgacgga agggaacgaa catggtcagg cgttccgcga tgtccagctg
    91981 gaacaggaac tgctggaaga ggcccagaaa ctcggtcttg gcgcgcagtt tggcggtaaa
    92041 tacttcgcgc acgacattcg cgttatccgt ctgccacgtc acggcgcatc ctgcccggta
    92101 ggcatgggcg tctcctgctc cgctgaccgt aacattaaag cgaaaatcaa ccgcgaaggt
    92161 atctggatcg aaaaactgga acaccatcca ggtcagtaca ttccacaaga actgcgccag
    92221 gccggtgaag gcgaagcggt gaaggtcgac cttaaccgcc cgatgaaaga gatcctcgcc
    92281 cagctttcgc aatatccggt ttccactcgt ttgtcgctca ccggcaccat tatcgtgggc
    92341 cgagatattg cacacgccaa gctgaaagag ctgattgacg ccggtaaaga acttccgcag
    92401 tacatcaaag atcacccgat ctactacgcg ggtccggcga aaacccctgc cggttatcca
    92461 tcaggctcac ttggcccaac caccgcaggt cgtatggact cctatgtgga tctgctgcaa
    92521 tcccacggcg gcagcatgat catgctagcg aaaggtaacc gcagtcagca ggttaccgac
    92581 gcgtgtcata aacacggcgg cttctacctc ggtagcatcg gcggtccggc ggcggtactg
    92641 gcgcagcaga gcatcaagca tctggaatgc gtcgcttatc cggagctggg tatggaagct
    92701 atctggaaaa tcgaagtaga agatttcccg gcgtttatcc tggtcgatga caaaggtaac
    92761 gacttcttcc agcaaatcat caacaaacag tgcgcgaact gcactaagta acctcttcgg
    92821 cccagcgcct ggcagcatgc tgccaggtga tccccctggg ccacctcttt tgcgattgta
    92881 atttcacgct tgctggtgaa tagtcagtat tttccctgac ttgcgaactc accatgaccc
    92941 gcacactcaa gccgttaatt cttaacaccg gcgcactggc gctaacgtta atcctgattt
    93001 ataccggcat ttcggcccat gacaaactca cctggctgat ggaagtgaca ccggtgatta
    93061 ttgtcgtgcc gctactgctt gccaccgcta aacgttatcc gttaacgccg ctgctctata
    93121 cgctcatttt ctttcacgcc atcatcctga tggtcggcgg gcaatacacc tacgcgaaag
    93181 tccccatcgg ttttgaggtg caggaatggc tggggttgag ccgtaatccg tatgacaagc
    93241 tggggcattt tttccagggg ctggtgcctg cactggtggc acgagagatc cttgtgcgcg
    93301 ggatgtacgt tcgcggacgt aaaatggtgg cgtttctggt ctgttgcgtg gcgctggcga
    93361 taagcgcgat gtatgaatta atcgagtggt gggcggcgct ggcaatgggc cagggagcgg
    93421 atgattttct cggcacccag ggcgaccagt gggacacgca atccgatatg ttctgcgcgc
    93481 tgcttggcgc attaaccacg gtgatattcc tcgctcgctt tcattgccgt cagctacggc
    93541 gctttggctt gataaccgga taacaacgtg cactttgttg atgccggatg cggcgtgaat
    93601 tccttatccg gtctacaaaa tttgcaaatc caaagcattc cattaagacg tggcctgata
    93661 aacatatcgc atcaggccat tttcgttagc tattgtccac gtcaggcttt cacatcactc
    93721 actgcaccga ccggaatatc agcatgaaca ggctcgggcg gtattttgcg atatttatcc
    93781 agcagatgaa tctggatcct gcgcagcgtg tcgccattga ggcgatagaa acggaagtag
    93841 agaatcagcg ttaccatgaa aaacagagtc ggtagagcaa tcataataaa ctgcatacct
    93901 aatagggctt gcgtagactg ttcaacattc ggtacatagc caatcatccc taacacaacc
    93961 gcaatgaaaa aagccgcaaa ggctgaaccg cccttcacca ccatagtctg cacggagtaa
    94021 gcgatgcttt cacagcgtac gtgcagttta tattcaccgt aatcaacggt atctgccacc
    94081 atgatgacct gtaataccca gaaaagcgcc gttcccacat tcagcaaaat acccgcaatc
    94141 acaatgagga cgacgttgtg atagctcatt aatgccatta acaggagaac accacagctt
    94201 aacaccggaa gaatagatgc tccggcccat aaaatgcgtc gggataatga tttaactaag
    94261 cgtgggaaga atactaacgt caccaggtta gcagctcccg catacgacag ataatagggg
    94321 aacaaatccg catcaccgat aacatatgag aaataataga tagcaaagcc ggtaataatg
    94381 ttgctggcta cattataagc aagagccata cccaagaggc atgaaagctg atcgttttta
    94441 taaataagcg caacgatggc tttaagtgtc agatggcttc cttcagcaga cggttgattg
    94501 tctgacgaaa agacttcatg cacattgcgc agagtgatga tggttgaaac aataaaaaag
    94561 gcgatcagta ccagtgtgaa catctgaaag ccaaatcccc gatcgccacc gccgacataa
    94621 ttaacaaatg gtagcgtcac acctgccgta acaaagcccg ccagactggc aaaaaaacgc
    94681 ggataaggaa ccagttgttc acgctcacgt ttatcgagcg tgatggttgg aaccagcgac
    94741 cagaagggaa tatccataat ggtgtaagtc atgccccaga ggatgtaggt cacgcaaaca
    94801 aaggcaatct gagtagtacc ttcaaacaga tgcgcactaa agaggagaaa taagattaca
    94861 gagtttgcca acgtaccgat caggatccag ggtttgaact taccccatcg cgatcgcgta
    94921 gcatttacaa tccatcccat aatcggatcg ttaatagcat cccagatcct cgccaccaga
    94981 aacaaagtac cgaccaaacc cacagacagc ccgacgacat cggtgtagta atacatgagg
    95041 tacatataca caatgccgat cgcaaaatcc ttcccgaacg ctccaaatcc ataactgagt
    95101 tttgtagtca ttgaaatgct catagggtat cgggtcgctg ttaccaacgc ccgccttctg
    95161 ttattggtat cgtcagaggt gtgcgccgga ataaatcccc cggcgcagta gcgtttagtc
    95221 gcgttttaac ggtgcaacca gcctggcagc cagtcgccgt gggcggcaat caggtcgtca
    95281 acaagagcat atatttcgtc aatgcccagc acggcggcag tatgcgggtc catcatcgca
    95341 gcgtggtaaa cacggtcgcg attttccgta agaatagctt cggtcagcag cgtctgtacg
    95401 ttgatgttgg tttgcatcag ggcggccaga tgcgaaggta gcgtaccgac tttggtcggc
    95461 tgaatgccat tagcatcaac cagacaggcc acttccacgc aacatccttg tggcaggtta
    95521 tcaatcaaac catcgttacg gacgttgccg taaatcacac tcggctcgcc cgtccagata
    95581 gcgttcatga ttgtgctggc atattcccgt gacggtttaa tatcaatccg ggaggcgttt
    95641 ttatactcct ccagctcttt atgccagttc gccagctgct cgacgcagcg tttcgggtac
    95701 tcatccagcg gtactttata acgctcaatc aaatcctcac gacctggctt aataaaccac
    95761 ggtgtgtact cagcaaaatg ttctgacgat tccgtgacga aatagcccag ctttttgaac
    95821 atttcatagc gcacaatatt ctggcagcga gtattgccgt gaatattcgg cttcggtgcc
    95881 tgccctgcgt cataagccgc cagcagttcc gggtagagat tcacgtaact gccgtcggcg
    95941 gttttgcgct ccagctccag gtaaaacgcc atatggttga tacctgcgca acggtaacgc
    96001 agcgtagccg ggtcgatatt gaggtcacgc gccagctctt ccgccgttcc ctgcaccgaa
    96061 tggcacagcc cgacctgttt gatatgcgga tagcgggcat acatcgccca ggtattcatc
    96121 gccattgggt taacatagtt gagcatggtg gcatcggggc agacttccgt catgtcctcg
    96181 caaatttgcc acagatgcgg aatggtacgt agcgcgcgca taataccgcc cggccccaac
    96241 gtatcggcaa tggtttgttc cagaccatgc cgcttacaga cctcgaaatc agtcaccgtg
    96301 caaggtgcat aaccgccaat ctgaaatgcc accacgacaa aatcggcatc ctgtaaggct
    96361 tctttctgtt gggtgtggca ggtgattttg ccgctggccc ctgctgaatc catcagctta
    96421 cgcaccacaa tatgcgactc ttccaggcgg gtggggtcaa tgtccatcag ggcaatatgc
    96481 gccgttttca gcgcctcgcg atggaacaca tcaccaagaa tatttttaac gaaaatcgtc
    96541 gaaccagcgc cgataaatgt aattttgggt gcagacatca tgcagatctc ctggcttgct
    96601 tgaataactt catcatggca ggcgaatctg cagaaaacct ccggcttcca ggttgagcat
    96661 tcccataaac tcagatcctg cgtgaagcag cagtaaatct gagtttatgg gaataattta
    96721 cctgaaaagc agagttttcg gcctcatgat gctcggacac tcccctcgcc cctctgggaa
    96781 gagggtgaaa accgtgctcc cactcgcagt catcctccct cactcctgcc ataattctga
    96841 tattccagga aagagagcca tccatgaata cagatacgtt tatgtgcagc agcgacgaaa
    96901 aacagacccg cagcccgctg tcgctgtatt cggaatacca gcggatggag attgagtttc
    96961 gcgcaccgca tatcatgccc accagccact ggcatggtca ggtcgaagtg aatgtgcctt
    97021 tcgatggcga tgtggaatac ctgatcaaca atgaaaaagt gaatatcaat cagggacata
    97081 tcacgctgtt ctgggcctgt ataccgcacc aactaacaga taccggaacc tgtcagagca
    97141 tggcgatttt taatctgccg atgcatctgt ttctctcctg gccgctggat aaagacctga
    97201 ttaaccacgt cactcacggc atggtgatca aatcacttgc tacacagcaa cttagcccgt
    97261 ttgaagtgcg ccgctggcag caggaattaa acagtccgaa tgagcaaatt cgccagctcg
    97321 ccattgatga aattggcctg atgctcaagc gatttagcct ctctggctgg gaaccgattc
    97381 tggtcaataa aacctcgcgc acacacaaaa acagcgtctc gcgccatgcg caattttatg
    97441 ttagccagat gctgggcttt attgccgaaa actatgacca ggcgctgacc atcaacgatg
    97501 tggctgagca cgtcaaactt aacgccaact atgcaatggg gatattccag cgggtcatgc
    97561 aattgacgat gaaacagtac attaccgcga tgcgcatcaa ccacgttcgc gcgttactga
    97621 gcgataccga taaaagtatt ctcgatattg ccctgacggc aggctttcgt tcaagtagtc
    97681 gtttttacag cacgttcggc aaatatgtcg gcatgtcgcc gcaacaatac cgcaaactta
    97741 gccaacagcg ccgccagacg tttcccggct aaagacgata tcagtatcag ccaaaaaaat
    97801 agtttagccg tcgataataa gcagtgaaaa taatttaaaa taatcacata acatattgtt
    97861 tataaaaaca aatatttcaa aaatgtttgt ttttcacgcg ctttacagcc cgaaaaggcc
    97921 ggaagatact tgcccgcaac gaagattcct tcataaccgg gtaagcaatg atgaaagtat
    97981 taattgttga aagcgagttt ctccatcaag acacctgggt cggtaacgct gttgagcgtc
    98041 tggcagatgc tttaagccag caaaatgtta ccgtgattaa atccacctcc tttgatgatg
    98101 gttttgccat tctctcttca aacgaagcca ttgactgcct gatgttcagc tatcaaatgg
    98161 aacatccgga cgaacatcaa aacgtcagac aattgatcgg taagcttcat gagcgccaac
    98221 aaaacgtgcc ggtcttcctg ttgggcgatc gggaaaaagc cctcgccgca atggaccggg
    98281 atctgctgga gcttgtcgat gaattcgcct ggattctgga agataccgcc gactttatcg
    98341 ccggacgcgc cgttgccgcg atgacccgct accgccagca gctgttgccg ccactgttca
    98401 gcgcgctgat gaaatatagc gacatccatg aatattcctg ggcagcgcca ggccaccagg
    98461 gcggcgttgg ttttaccaaa acacccgccg gacgtttcta ccatgactac tacggtgaaa
    98521 atctgttccg caccgacatg ggcatcgaac gaacttccct cggttctttg cttgaccata
    98581 ctggcgcatt tggcgaaagc gaaaaatatg ccgcacgcgt atttggtgcc gatcgctcct
    98641 ggtcggtagt cgtcggtact tccggctcta accgcaccat catgcaggct tgcatgaccg
    98701 ataacgatgt cgtggtcgtt gaccgtaact gccataaatc catcgaacaa ggtttgatgc
    98761 tgacaggcgc gaaaccggtc tacatggtgc caagccgcaa ccgctacggc attatcgggc
    98821 caatctatcc gcaggaaatg caacctgaaa ccttgcagaa gaaaatcagt gaaagcccgc
    98881 tgactaaaga caaagccggg caaaaaccgt cttactgcgt ggtgaccaac tgcacctatg
    98941 acggcgtgtg ttataacgct aaagaagcgc aggatctgct ggaaaaaacc tccgatcgtc
    99001 tgcactttga cgaagcctgg tacggctatg cacgtttcaa cccgatctat gccgatcact
    99061 atgccatgcg cggcgaacct ggcgatcaca acggtcctac cgttttcgcc acccactcca
    99121 cccacaaact gctgaatgcg ctttcacagg cctcttatat tcatgtacgt gaaggtcgtg
    99181 gggcgattaa cttctcccgc ttcaaccagg cctacatgat gcatgccacc acctccccgc
    99241 tgtatgccat ctgcgcatcc aacgacgtgg cggtgtcgat gatggacggc aacagcggcc
    99301 tgtcactgac gcaggaagtg atcgacgaag cggttgattt ccgtcaggcg atggcgcggc
    99361 tgtataaaga gttcgccgct gacggtagct ggttcttcaa accgtggaac aaagaagtcg
    99421 tcaccgaccc acaaaccggc aaaacctatg actttgctga cgcaccaacc aaactgctga
    99481 ccaccgttca ggactgctgg gtaatgcatc cgggcgaaag ctggcacggc ttcaaagata
    99541 ttccggataa ctggagtatg ctcgacccga ttaaagtcag catccttgct ccgggaatgg
    99601 gtgaagatgg tgaactggaa gaaaccggtg ttccggcggc gctggtcact gcctggcttg
    99661 gtcgccacgg cattgtgcct acccgcacca ctgacttcca aattatgttc ctgttctcta
    99721 tgggcgtaac ccgtgggaaa tgggggactc tggttaacac cctttgctcc ttcaaacgcc
    99781 actatgacgc caacacaccg ctggcgcagg tgatgccgga acttgttgaa caatatcctg
    99841 acacttacgc gaacatgggg attcacgatc tgggtgacac catgtttgcc tggctgaaag
    99901 aaaacaaccc tggcgcacgg ttgaacgaag cctattccgg cctgccggtg gcggaaatca
    99961 ccccgcgtga agcgtacaac gcgattgtcg acaacaatgt cgaactggta tccattgaaa
   100021 atctgccagg acgtatcgcg gcaaactcag ttatcccgta tccgccagga atcccgatgc
   100081 tgctgtctgg tgaaaacttc ggcgataaaa acagtccgca agtaagttat ttacgctcgc
   100141 tgcaatcctg ggaccatcat ttccctggat ttgaacacga aactgaaggg actgaaatta
   100201 ttgacggtat ttaccacgtt atgtgtgtga aagcgtaacc actattccgc tgaaggcgta
   100261 attgtttaaa taacattacg ccgcctggcc ttaggccttt tgagtatggc aacgttttca
   100321 taaaaattgc tgcaaacaaa aatgtcatac tttttgcgcg gccccacccc gcgctttttt
   100381 ttgcctgtt
//
LOCUS       DAZKIP010000004        96545 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZKIP010000004 DAZKIP010000000
VERSION     DAZKIP010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 96545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 96545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..96545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.004"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            187..813
                     /gene="ydhY"
                     /locus_tag="ACHOUK_000296"
     CDS             187..813
                     /gene="ydhY"
                     /locus_tag="ACHOUK_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFP9895821.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            834..2936
                     /gene="ydhV"
                     /locus_tag="ACHOUK_000297"
     CDS             834..2936
                     /gene="ydhV"
                     /locus_tag="ACHOUK_000297"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFP9895822.1"
                     /translation="MTNGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAECWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            2949..3587
                     /gene="ydhW"
                     /locus_tag="ACHOUK_000298"
     CDS             2949..3587
                     /gene="ydhW"
                     /locus_tag="ACHOUK_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFP9895823.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSVAGQLVARAVFLSPPYSVAEEELSVLLENIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            3651..4319
                     /gene="ydhX"
                     /locus_tag="ACHOUK_000299"
     CDS             3651..4319
                     /gene="ydhX"
                     /locus_tag="ACHOUK_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310405.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFP9895824.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHAPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4316..5101
                     /gene="phsC"
                     /locus_tag="ACHOUK_000300"
     CDS             4316..5101
                     /gene="phsC"
                     /locus_tag="ACHOUK_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFP9895825.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGERIYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINGVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5105..5917
                     /gene="ydhT"
                     /locus_tag="ACHOUK_000301"
     CDS             5105..5917
                     /gene="ydhT"
                     /locus_tag="ACHOUK_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFP9895826.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGERAFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGKRCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(5929..7533)
                     /gene="ydhS"
                     /locus_tag="ACHOUK_000302"
     CDS             complement(5929..7533)
                     /gene="ydhS"
                     /locus_tag="ACHOUK_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFP9895827.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARKQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDQFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(7659..7964)
                     /gene="ydhR"
                     /locus_tag="ACHOUK_000303"
     CDS             complement(7659..7964)
                     /gene="ydhR"
                     /locus_tag="ACHOUK_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFP9895828.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTAQLKKLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8072..8148)
                     /locus_tag="ACHOUK_000304"
     tRNA            complement(8072..8148)
                     /locus_tag="ACHOUK_000304"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8112..8114),aa:Val,seq:gac)
     gene            complement(8153..8229)
                     /locus_tag="ACHOUK_000305"
     tRNA            complement(8153..8229)
                     /locus_tag="ACHOUK_000305"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8193..8195),aa:Val,seq:gac)
     gene            8537..9793
                     /gene="ydhQ"
                     /locus_tag="ACHOUK_000306"
     CDS             8537..9793
                     /gene="ydhQ"
                     /locus_tag="ACHOUK_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFP9895829.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKANDTLLADGTMNNLGGEDSDTIVENGAIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFSIGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVQITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(9834..11207)
                     /gene="mdtK"
                     /locus_tag="ACHOUK_000307"
     CDS             complement(9834..11207)
                     /gene="mdtK"
                     /locus_tag="ACHOUK_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFP9895830.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRYLQRLPSAI
                     ILQRASR"
     gene            11422..12063
                     /gene="ribE"
                     /locus_tag="ACHOUK_000308"
     CDS             11422..12063
                     /gene="ribE"
                     /locus_tag="ACHOUK_000308"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFP9895831.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(12103..13251)
                     /gene="cfa"
                     /locus_tag="ACHOUK_000309"
     CDS             complement(12103..13251)
                     /gene="cfa"
                     /locus_tag="ACHOUK_000309"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFP9895832.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFTVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFVAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13542..14753)
                     /gene="punC"
                     /locus_tag="ACHOUK_000310"
     CDS             complement(13542..14753)
                     /gene="punC"
                     /locus_tag="ACHOUK_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFP9895833.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAAMLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            14866..15798
                     /gene="punR"
                     /locus_tag="ACHOUK_000311"
     CDS             14866..15798
                     /gene="punR"
                     /locus_tag="ACHOUK_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFP9895834.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(15795..16820)
                     /gene="purR"
                     /locus_tag="ACHOUK_000312"
     CDS             complement(15795..16820)
                     /gene="purR"
                     /locus_tag="ACHOUK_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFP9895835.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17119..17208
                     /gene="cydH"
                     /locus_tag="ACHOUK_000313"
                     /gene_synonym="ynhF"
     CDS             17119..17208
                     /gene="cydH"
                     /locus_tag="ACHOUK_000313"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFP9895836.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            17374..18543
                     /gene="ydhP"
                     /locus_tag="ACHOUK_000314"
     CDS             17374..18543
                     /gene="ydhP"
                     /locus_tag="ACHOUK_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9895837.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNKFGAAISMAVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(18689..19270)
                     /gene="sodB"
                     /locus_tag="ACHOUK_000315"
     CDS             complement(18689..19270)
                     /gene="sodB"
                     /locus_tag="ACHOUK_000315"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFP9895838.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19398..20213)
                     /gene="mepH"
                     /locus_tag="ACHOUK_000316"
     CDS             complement(19398..20213)
                     /gene="mepH"
                     /locus_tag="ACHOUK_000316"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFP9895839.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHTVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20548..20895
                     /gene="grxD"
                     /locus_tag="ACHOUK_000317"
     CDS             20548..20895
                     /gene="grxD"
                     /locus_tag="ACHOUK_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFP9895840.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(20946..25562)
                     /gene="lhr"
                     /locus_tag="ACHOUK_000318"
     CDS             complement(20946..25562)
                     /gene="lhr"
                     /locus_tag="ACHOUK_000318"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFP9895841.1"
                     /translation="MADNPNPSSPLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAADAAHFE
                     STSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVELVIQVATPLSVASGLQRIGRAGHQIGGISKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQHTVAAAAMDALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTIANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPAILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEIASYLE
                     NLLTVKRIFPTMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVHDYSPEVLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDPSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPRVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQAAQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVWQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(25655..26302)
                     /gene="rnt"
                     /locus_tag="ACHOUK_000319"
     CDS             complement(25655..26302)
                     /gene="rnt"
                     /locus_tag="ACHOUK_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFP9895842.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(26405..26812)
                     /gene="gloA"
                     /locus_tag="ACHOUK_000320"
     CDS             complement(26405..26812)
                     /gene="gloA"
                     /locus_tag="ACHOUK_000320"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFP9895843.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(26893..27990)
                     /gene="nemA"
                     /locus_tag="ACHOUK_000321"
     CDS             complement(26893..27990)
                     /gene="nemA"
                     /locus_tag="ACHOUK_000321"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFP9895844.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPSI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(28027..28626)
                     /gene="nemR"
                     /locus_tag="ACHOUK_000322"
     CDS             complement(28027..28626)
                     /gene="nemR"
                     /locus_tag="ACHOUK_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFP9895845.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRDSHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNSEPLENALAHVKTIIATPAV"
     gene            28729..28968
                     /gene="ydhL"
                     /locus_tag="ACHOUK_000323"
     CDS             28729..28968
                     /gene="ydhL"
                     /locus_tag="ACHOUK_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFP9895846.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            29017..29913
                     /gene="ydhF"
                     /locus_tag="ACHOUK_000324"
                     /pseudo
     CDS             29017..29913
                     /gene="ydhF"
                     /locus_tag="ACHOUK_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
     gene            29994..30515
                     /gene="sodC"
                     /locus_tag="ACHOUK_000325"
     CDS             29994..30515
                     /gene="sodC"
                     /locus_tag="ACHOUK_000325"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFP9895847.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(30516..32528)
                     /gene="ydhK"
                     /locus_tag="ACHOUK_000326"
     CDS             complement(30516..32528)
                     /gene="ydhK"
                     /locus_tag="ACHOUK_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP9895848.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDVYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSDVDDHARLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYNFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGIVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(32528..33385)
                     /gene="ydhJ"
                     /locus_tag="ACHOUK_000327"
     CDS             complement(32528..33385)
                     /gene="ydhJ"
                     /locus_tag="ACHOUK_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFP9895849.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(33388..33624)
                     /gene="ydhI"
                     /locus_tag="ACHOUK_000328"
     CDS             complement(33388..33624)
                     /gene="ydhI"
                     /locus_tag="ACHOUK_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFP9895850.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            33825..34259
                     /gene="slyA"
                     /locus_tag="ACHOUK_000329"
     CDS             33825..34259
                     /gene="slyA"
                     /locus_tag="ACHOUK_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFP9895851.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(34306..34773)
                     /gene="slyB"
                     /locus_tag="ACHOUK_000330"
     CDS             complement(34306..34773)
                     /gene="slyB"
                     /locus_tag="ACHOUK_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFP9895852.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            35046..36155
                     /gene="anmK"
                     /locus_tag="ACHOUK_000331"
     CDS             35046..36155
                     /gene="anmK"
                     /locus_tag="ACHOUK_000331"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFP9895853.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVKALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            36253..36582
                     /gene="mliC"
                     /locus_tag="ACHOUK_000332"
     CDS             36253..36582
                     /gene="mliC"
                     /locus_tag="ACHOUK_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFP9895854.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGLSASGARYSDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            36641..37297
                     /gene="pdxH"
                     /locus_tag="ACHOUK_000333"
     CDS             36641..37297
                     /gene="pdxH"
                     /locus_tag="ACHOUK_000333"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFP9895855.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGSWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            37426..38700
                     /gene="tyrS"
                     /locus_tag="ACHOUK_000334"
     CDS             37426..38700
                     /gene="tyrS"
                     /locus_tag="ACHOUK_000334"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFP9895856.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            38762..39622
                     /gene="pdxY"
                     /locus_tag="ACHOUK_000335"
     CDS             38762..39622
                     /gene="pdxY"
                     /locus_tag="ACHOUK_000335"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HFP9895857.1"
                     /translation="MKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKAA
                     NPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHPVNNV
                     EEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQP
                     VGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIAK
                     PEHYFSATKL"
     gene            complement(39666..40271)
                     /gene="gstA"
                     /locus_tag="ACHOUK_000336"
     CDS             complement(39666..40271)
                     /gene="gstA"
                     /locus_tag="ACHOUK_000336"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFP9895858.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(40377..41879)
                     /gene="dtpA"
                     /locus_tag="ACHOUK_000337"
     CDS             complement(40377..41879)
                     /gene="dtpA"
                     /locus_tag="ACHOUK_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFP9895859.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHTILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(42490..43125)
                     /gene="nth"
                     /locus_tag="ACHOUK_000338"
     CDS             complement(42490..43125)
                     /gene="nth"
                     /locus_tag="ACHOUK_000338"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFP9895860.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNS
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(43125..43820)
                     /gene="rsxE"
                     /locus_tag="ACHOUK_000339"
     CDS             complement(43125..43820)
                     /gene="rsxE"
                     /locus_tag="ACHOUK_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFP9895861.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(43824..44444)
                     /gene="rsxG"
                     /locus_tag="ACHOUK_000340"
     CDS             complement(43824..44444)
                     /gene="rsxG"
                     /locus_tag="ACHOUK_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFP9895862.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGNFDQFTGATITPRAVVNAVKRAGLYAQTLPEQLSQLPACGE"
     gene            complement(44448..45506)
                     /gene="rsxD"
                     /locus_tag="ACHOUK_000341"
     CDS             complement(44448..45506)
                     /gene="rsxD"
                     /locus_tag="ACHOUK_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFP9895863.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(45507..47825)
                     /gene="rsxC"
                     /locus_tag="ACHOUK_000342"
     CDS             complement(45507..47825)
                     /gene="rsxC"
                     /locus_tag="ACHOUK_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFP9895864.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRSRRREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDISMRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVTDPRKTAVEAAIARA
                     KARKLEQQQANAEPEQQVDPRKAAVEAAIARAKARKLEQQQANAEPEQQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEPVDPRKAAVEAAIARAKARKLEQQQANAEPEEPVDP
                     RKAAVEAAITRAKARKLEQQQANAEPEEQVDPRKAAVAAAIARAKARKLEQQQANAEP
                     EEQVDPRKAAVAAAIARVQAKKAVQQKVVNED"
     gene            complement(47818..48396)
                     /gene="rsxB"
                     /locus_tag="ACHOUK_000343"
     CDS             complement(47818..48396)
                     /gene="rsxB"
                     /locus_tag="ACHOUK_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFP9895865.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(48396..48977)
                     /gene="rsxA"
                     /locus_tag="ACHOUK_000344"
     CDS             complement(48396..48977)
                     /gene="rsxA"
                     /locus_tag="ACHOUK_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFP9895866.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(49054..49494)
                     /gene="ydgK"
                     /locus_tag="ACHOUK_000345"
     CDS             complement(49054..49494)
                     /gene="ydgK"
                     /locus_tag="ACHOUK_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFP9895867.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGAQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(49580..49795)
                     /gene="ydgT"
                     /locus_tag="ACHOUK_000346"
     CDS             complement(49580..49795)
                     /gene="ydgT"
                     /locus_tag="ACHOUK_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFP9895868.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(50068..50193)
                     /gene="blr"
                     /locus_tag="ACHOUK_000347"
     CDS             complement(50068..50193)
                     /gene="blr"
                     /locus_tag="ACHOUK_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFP9895869.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSTTVQHK"
     gene            50436..51476
                     /gene="ydgJ"
                     /locus_tag="ACHOUK_000348"
     CDS             50436..51476
                     /gene="ydgJ"
                     /locus_tag="ACHOUK_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFP9895870.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(51512..52513)
                     /gene="add"
                     /locus_tag="ACHOUK_000349"
     CDS             complement(51512..52513)
                     /gene="add"
                     /locus_tag="ACHOUK_000349"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFP9895871.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLNAEEKRALREKVAAK"
     gene            complement(52617..53789)
                     /gene="malY"
                     /locus_tag="ACHOUK_000350"
     CDS             complement(52617..53789)
                     /gene="malY"
                     /locus_tag="ACHOUK_000350"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFP9895872.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDPQSVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(53799..55391)
                     /gene="malX"
                     /locus_tag="ACHOUK_000351"
     CDS             complement(53799..55391)
                     /gene="malX"
                     /locus_tag="ACHOUK_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFP9895873.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLLPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVVAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            55566..56594
                     /gene="malI"
                     /locus_tag="ACHOUK_000352"
     CDS             55566..56594
                     /gene="malI"
                     /locus_tag="ACHOUK_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFP9895874.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            56706..57473
                     /gene="hdhA"
                     /locus_tag="ACHOUK_000353"
     CDS             56706..57473
                     /gene="hdhA"
                     /locus_tag="ACHOUK_000353"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFP9895875.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            57702..58292
                     /gene="uidR"
                     /locus_tag="ACHOUK_000354"
     CDS             57702..58292
                     /gene="uidR"
                     /locus_tag="ACHOUK_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFP9895876.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            58683..60496
                     /gene="uidA"
                     /locus_tag="ACHOUK_000355"
                     /pseudo
     CDS             58683..60496
                     /gene="uidA"
                     /locus_tag="ACHOUK_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
     gene            60493..61866
                     /gene="uidB"
                     /locus_tag="ACHOUK_000356"
     CDS             60493..61866
                     /gene="uidB"
                     /locus_tag="ACHOUK_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFP9895877.1"
                     /translation="MNQQLSWRTIVGYSLGDIANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            61905..63169
                     /gene="uidC"
                     /locus_tag="ACHOUK_000357"
                     /pseudo
     CDS             61905..63169
                     /gene="uidC"
                     /locus_tag="ACHOUK_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
     gene            complement(63214..64722)
                     /gene="ydgA"
                     /locus_tag="ACHOUK_000358"
     CDS             complement(63214..64722)
                     /gene="ydgA"
                     /locus_tag="ACHOUK_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFP9895878.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(64823..65998)
                     /gene="manA"
                     /locus_tag="ACHOUK_000359"
     CDS             complement(64823..65998)
                     /gene="manA"
                     /locus_tag="ACHOUK_000359"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFP9895879.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HKSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPDDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            66197..67843
                     /gene="fumB"
                     /locus_tag="ACHOUK_000360"
     CDS             66197..67843
                     /gene="fumB"
                     /locus_tag="ACHOUK_000360"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFP9895880.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSLSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQALGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            67986..69389
                     /gene="fumC"
                     /locus_tag="ACHOUK_000361"
     CDS             67986..69389
                     /gene="fumC"
                     /locus_tag="ACHOUK_000361"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFP9895881.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(69386..70315)
                     /gene="tus"
                     /locus_tag="ACHOUK_000362"
     CDS             complement(69386..70315)
                     /gene="tus"
                     /locus_tag="ACHOUK_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFP9895882.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSQALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(70391..71692)
                     /gene="rstB"
                     /locus_tag="ACHOUK_000363"
     CDS             complement(70391..71692)
                     /gene="rstB"
                     /locus_tag="ACHOUK_000363"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFP9895883.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(71696..72415)
                     /gene="rstA"
                     /locus_tag="ACHOUK_000364"
     CDS             complement(71696..72415)
                     /gene="rstA"
                     /locus_tag="ACHOUK_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFP9895884.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDIQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            72544..72879
                     /gene="ydgC"
                     /locus_tag="ACHOUK_000365"
     CDS             72544..72879
                     /gene="ydgC"
                     /locus_tag="ACHOUK_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFP9895885.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(72876..73598)
                     /gene="folM"
                     /locus_tag="ACHOUK_000366"
     CDS             complement(72876..73598)
                     /gene="folM"
                     /locus_tag="ACHOUK_000366"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFP9895886.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(73635..75017)
                     /gene="ydgI"
                     /locus_tag="ACHOUK_000367"
     CDS             complement(73635..75017)
                     /gene="ydgI"
                     /locus_tag="ACHOUK_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9895887.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDIFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(75203..76147)
                     /gene="ydgH"
                     /locus_tag="ACHOUK_000368"
     CDS             complement(75203..76147)
                     /gene="ydgH"
                     /locus_tag="ACHOUK_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFP9895888.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            76671..78203
                     /gene="pntA"
                     /locus_tag="ACHOUK_000369"
     CDS             76671..78203
                     /gene="pntA"
                     /locus_tag="ACHOUK_000369"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFP9895889.1"
                     /translation="MRIGIPRERLTNEPRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGCFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKNGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQVAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            78214..79602
                     /gene="pntB"
                     /locus_tag="ACHOUK_000370"
     CDS             78214..79602
                     /gene="pntB"
                     /locus_tag="ACHOUK_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFP9895890.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(79627..80661)
                     /gene="tqsA"
                     /locus_tag="ACHOUK_000371"
     CDS             complement(79627..80661)
                     /gene="tqsA"
                     /locus_tag="ACHOUK_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFP9895891.1"
                     /translation="MAKPLITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSTLNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFKQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            81073..81438
                     /gene="mdtJ"
                     /locus_tag="ACHOUK_000372"
     CDS             81073..81438
                     /gene="mdtJ"
                     /locus_tag="ACHOUK_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFP9895892.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            81425..81754
                     /gene="mdtI"
                     /locus_tag="ACHOUK_000373"
     CDS             81425..81754
                     /gene="mdtI"
                     /locus_tag="ACHOUK_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFP9895893.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(81793..82614)
                     /gene="ydgD"
                     /locus_tag="ACHOUK_000374"
     CDS             complement(81793..82614)
                     /gene="ydgD"
                     /locus_tag="ACHOUK_000374"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFP9895894.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYDIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(82714..82797)
                     /locus_tag="ACHOUK_000375"
     CDS             complement(82714..82797)
                     /locus_tag="ACHOUK_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9895895.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(82890..83198)
                     /gene="asr"
                     /locus_tag="ACHOUK_000376"
     CDS             complement(82890..83198)
                     /gene="asr"
                     /locus_tag="ACHOUK_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFP9895896.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAKKHSHQQPAKPAAQPAA
                     "
     gene            83469..83573
                     /locus_tag="ACHOUK_000377"
     CDS             83469..83573
                     /locus_tag="ACHOUK_000377"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFP9895897.1"
                     /translation="MNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            complement(83622..84875)
                     /gene="ynfM"
                     /locus_tag="ACHOUK_000378"
     CDS             complement(83622..84875)
                     /gene="ynfM"
                     /locus_tag="ACHOUK_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9895898.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKSLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            84982..85875
                     /gene="ynfL"
                     /locus_tag="ACHOUK_000379"
     CDS             84982..85875
                     /gene="ynfL"
                     /locus_tag="ACHOUK_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9895899.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            86010..87230
                     /gene="mlc"
                     /locus_tag="ACHOUK_000380"
                     /gene_synonym="dgsA"
     CDS             86010..87230
                     /gene="mlc"
                     /locus_tag="ACHOUK_000380"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFP9895900.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            87355..88050
                     /gene="bioD"
                     /locus_tag="ACHOUK_000381"
     CDS             87355..88050
                     /gene="bioD"
                     /locus_tag="ACHOUK_000381"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFP9895901.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TEKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(88003..89295)
                     /gene="clcB"
                     /locus_tag="ACHOUK_000382"
     CDS             complement(88003..89295)
                     /gene="clcB"
                     /locus_tag="ACHOUK_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFP9895902.1"
                     /translation="MHHLHTYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(89453..90067)
                     /gene="dmsD"
                     /locus_tag="ACHOUK_000383"
     CDS             complement(89453..90067)
                     /gene="dmsD"
                     /locus_tag="ACHOUK_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFP9895903.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(90110..90964)
                     /gene="ynfH"
                     /locus_tag="ACHOUK_000384"
     CDS             complement(90110..90964)
                     /gene="ynfH"
                     /locus_tag="ACHOUK_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFP9895904.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKTDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFVTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLSSIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(90966..91583)
                     /gene="ynfG"
                     /locus_tag="ACHOUK_000385"
     CDS             complement(90966..91583)
                     /gene="ynfG"
                     /locus_tag="ACHOUK_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFP9895905.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(91594..94017)
                     /gene="ynfF"
                     /locus_tag="ACHOUK_000386"
     CDS             complement(91594..94017)
                     /gene="ynfF"
                     /locus_tag="ACHOUK_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFP9895906.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLISENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTNEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            complement(94078..96504)
                     /gene="ynfE"
                     /locus_tag="ACHOUK_000387"
     CDS             complement(94078..96504)
                     /gene="ynfE"
                     /locus_tag="ACHOUK_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
                     /protein_id="HFP9895907.1"
                     /translation="MSKNERMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQASEQVVWGACSVNCGSRCALRLHVKDNEVAWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNITRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKSREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     STFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVYNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
BASE COUNT        24207 a        23881 c        25065 g        23392 t
ORIGIN      
        1 ataaccacat tattttttct cctaaccata aaggattagt ttatcggcgt taaatatttt
       61 ctcatacact atcgggttag ggaaaatatc tggtgttggg gacagagtca gcagcgtatt
      121 agttgaatgc ttaatatcag gctgctgaat tgtcagcact gtgtttgcga agaggatgcg
      181 aaataaatga acccggttga tcgtccacta ttagatattg gcttaacgcg attagaattc
      241 ttgcgtatat caggaaaagg ccttgctggt ctaacaattg cccctgcgtt actgtcactt
      301 ttgggttgca aacaggaaga tattgatagc ggcacagtag ggttgataaa tacacccaaa
      361 ggggtgctgg tgacccagcg agcgcgatgc acgggttgcc accgctgtga aatctcttgt
      421 accaacttca acgatggctc agtagggaca ttcttctccc gtatcaaaat ccatcgcaat
      481 tatttctttg gcgacaacgg ggttggctct ggcggcggcc tgtatggcga tctcaactat
      541 accgcggaca cctgccgtca atgcaaagag ccgcaatgca tgaacgtctg cccgattggt
      601 gcgattacct ggcagcagaa agaaggctgt attaccgtcg atcataaacg ttgtattggc
      661 tgtagcgcct gtactacggc atgcccgtgg atgatggcca ccgtaaatac cgaaagtaaa
      721 aaatcctcga aatgtgtgtt atgcggtgaa tgcgcaaacg cctgcccaac aggggcgtta
      781 aaaattatcg agtggaaaga tattactgtg tgaatcttgc aaaggaaaaa atcatgacta
      841 acggttggac aggtaatata ttaagagtca atctcacgac aggaaatatt accctcgaag
      901 attccagtaa gtttaaaagt tttgtcggtg gcatgggctt cggctacaaa attatgtatg
      961 acgaagtacc gccaggcacg aaacctttcg atgaagcgaa taaattagtc tttgctaccg
     1021 gcccattaac tggatctggt gccccctgta gttctcgcgt aaatatcacc tcactttcta
     1081 cttttaccaa aggaaattta gtcgtcgatg cccatatggg tggctttttt gcagcgcaaa
     1141 tgaaattcgc tggatacgac gtcattatta tcgaagggaa ggcgaaatca ccggtatggc
     1201 tgaagattaa agatgacaaa gttagcctgg aaaaagcaga tttcttatgg ggaaaaggga
     1261 cgcgcgcaac gacggaagaa atttgtcgct tgaccagtcc ggaaacctgt gtggcggcta
     1321 ttggtcaggc tggggaaaac cttgttcctc tctctggcat gttgaatagc cgtaaccaca
     1381 gcggcggtgc gggaactggc gcaataatgg gttcgaaaaa cctgaaagcg attgcggttg
     1441 aagggacgaa aggggtcaac attgccgatc gtcaggagat gaagcgtctc aatgattaca
     1501 tgatgactga acttatcggt gcgaataaca accatgtcgt gccaagtacg ccgcaatcgt
     1561 gggcagagta ttcagatccc aagtcacgct ggacagcacg taaagggctg ttttggggcg
     1621 cggctgaagg cggtccaatt gaaacgggtg aaattccgcc aggcaatcag aatacggtcg
     1681 gctttcgtac ctataaatcc gtttttgact taggtccggc agcagagaaa tacacagtaa
     1741 aaatgagcgg ctgccactct tgcccgatcc gttgtatgac ccaaatgaat attcctcggg
     1801 tgaaagagtt tggcgtgccc agcacaggtg gtaacacttg tgtagcaaac tttgtccata
     1861 ccaccatctt cccgaacggg ccgaaagatt ttgaagataa agacgatggt cgtgtgattg
     1921 gtaacctggt tggtctgaat ttgttcgatg actacggcct atggtgtaac tacgggcagt
     1981 tgcatcgcga ctttacttat tgttacagca aaggtgtgtt caagcgtgtt ctgcctgctg
     2041 aagagtatgc agaaattcgc tgggatcaac tggaagcggg agacgttaac ttcattaaag
     2101 atttttacta ccgtctggcg catcgtgtgg gtgagctgag tcacctggct gatggttcat
     2161 atgccatcgc agaatgctgg aatttgggtg aagagtactg gggctacgcg aaaaataaac
     2221 tctggtcgcc gtttggctat ccggttcacc atgccaatga agcgtcagcg caggtcggtt
     2281 ccattgttaa ctgtatgttc aaccgtgact gcatgacgca tacccatatc aactttattg
     2341 gttccggctt gccattgaaa ctgcaacgtg aagtggcgaa agaacttttt ggttctgaag
     2401 atgcttacga tgaaaccaaa aactacacgc caatcaatga cgcaaaaatt aaatatgcta
     2461 agtggtcgct gttgcgggtc tgtttgcata acgccgtcac cctgtgcaac tgggtctggc
     2521 caatgaccgt ttcgccgctg aaaagccgta attaccgggg cgatctggcg cttgaagcca
     2581 aattcttcaa agcgatcact ggcgaagaga tgactcagga aaaattagat ttagctgcag
     2641 agcgtatctt tacattgcat cgtgcctaca cggtgaaact gatgcaaacc aaagatatgc
     2701 gtaatgaaca cgatcttatc tgttcctggg tattcgacaa ggatccgcag atcccggtct
     2761 ttactgaagg tactgacaaa atggatcgtg atgatatgca tgcctcgctc acgatgttct
     2821 acaaagaaat gggctgggac ccacagcttg gttgtccgac ccgcgaaaca ttgcagcgtc
     2881 tggggctgga agatattgcc gccgatctgg cagcacacaa tctactgcct gcgtaaggaa
     2941 tgggtaagat gaaccatcag gatgaattgc cgttagcgaa agtcagcgaa gttgacgagg
     3001 cgaagcggca atggttgcag gggatgcgtc accccgttga tacggtaacc gaaccagaac
     3061 cggctgaaat cctggcagag ttcattcgcc agcattctgt ggctgggcag ctggtggcgc
     3121 gcgccgtgtt tctctcgcca ccctattcag ttgcagaaga ggaactgtca gttttgctgg
     3181 aaaacatcaa gcagaacgga gactacgcgg atatcgcttg tatgacggga agtcaggatg
     3241 attactatta ctcaacgcag gccatgagtg aaaactacgc ggcgatgtct ttgcaagttg
     3301 tggagcagga catttgtcgc gccattgcac acgccgttcg ttttgagtgc cagacgtatc
     3361 ctcgtcctta caaagtcgcc atgctgatgc aagcgccata ttacttccag gaagcgcaga
     3421 ttgaagccgc gatcgccgca atggatgttg cacctgaata tgccgatata cggcaggtag
     3481 agtcatcgac ggcggtgttg tacttgttta gcgagcgatt tatgacctat ggcaaggctt
     3541 atggcttatg cgaatggttc gaggtagagc agtttcagaa cccctgaaat ttcagggaga
     3601 tgtcatggat agggtggaca gttgcagcca ctgcattagg ggataatcag atgtccttca
     3661 ctcgacgcaa atttgttctg gggatgggaa cagtaatctt ttttactggt tctgcgtctt
     3721 ctctgttagc gaacacgagg caagaaaagg aagttcggta tgccatgatt catgacgagt
     3781 cacggtgcaa tggctgtaat atttgcgccc gcgcatgccg taaaactaac cacgcccccg
     3841 ctcagggaag ccgtttatcg atagcacaca ttccagtcac cgataacgac aacgaaacgc
     3901 agtatcactt ttttcgccag tcatgtcagc actgcgaaga tgctccgtgc atcgacgtat
     3961 gcccgacagg ggcgtcgtgg cgtgatgagc aggggatcgt gcgggtagaa aaatcgcagt
     4021 gcatcggctg cagttattgc attggcgctt gtccgtacca ggttcgctac cttaaccctg
     4081 tcactaaagt ggcagataaa tgtgatttct gcgctgagtc ccgactggca aaaggctttc
     4141 cgcccatttg cgtcagcgct tgcccggaac acgcattgat ctttggtcgc gaagatagcc
     4201 ctgaaattca ggcgtggttg caggataata aatactatca atatcagcta cctggtgccg
     4261 ggaaaccgca tctgtatcgc cggttcggtc aacatttgat taaaaaggaa aatgtatgaa
     4321 cccgtcgcaa catgctgaac agtttcagag ccagttagcg aactatgtgc cacagttcac
     4381 tcccgaattt tggccggtgt ggttgatcat tgccggagta ttgctggttg ggatgtggtt
     4441 ggtgctgggg ctgcacgcct tgcttcgtgc tcgtggcgtg aagaaatcag ccaccgatca
     4501 tggtgagagg atttatcttt acagcaaagc ggtcagatta tggcactggt cgaatgcgtt
     4561 actctttgta ttgttgctgg caagtgggct gataaatcac tttgcgctgg tgggcgcaac
     4621 tgcggttaaa agtctggttg cggtgcatga agtttgcgga tttttgttac tggcatgctg
     4681 gctcggcttt gttctgatca atggcgttgg gggtaatggc caccactatc gcattcgtcg
     4741 tcagggttgg ctggagcggg cggcaaaaca aacgcgattt tatttgtttg gcattatgca
     4801 gggggaagaa catcctttcc cggcaacaac ccagtctaaa tttaatccct tacagcaggt
     4861 tgcctatgtt ggtgtcatgt atggattgct gccgttgtta ctattgacgg ggctgctgtg
     4921 tctctatccg caagccgtgg gagatgtgtt tcctggcgta agatactggt tattgcaggc
     4981 gcattttgct ctggcattta taagcctctt ttttatcttc ggtcatcttt atctttgcac
     5041 cacggggcgt acgccacacg aaacctttaa aagcatggtc gatggctatc accggcacta
     5101 acgaatgatc attacacgag cagacctgcg agaatggcgt atcggagcgg tgatgtatcg
     5161 ctggttcctg cgccatttcc cacgcggcgg tagttatgcc gatattcatc atgcactgat
     5221 tgaggaagga tatacggact gggcggagag tctggttgag tatgcgtgga aaaaatggct
     5281 ggcggatgaa aacttcgcgc atcaggaagt ttcctctatg caaaaactgg ccacagaccc
     5341 cggagagaga gcattttgca gtcagtttgc ccgcagcgac gatcatgctc gcatcggctg
     5401 ttgtgaggat aacgccagaa tcgcaaccgc tgggtatgct gcacaaattg ccagtatggg
     5461 ttatagcgtg cggattggaa gtgtcggctt taacagtcac attggcagct ccggtgagcg
     5521 cgcacgggtt gctgtaaccg gcaattcttc gcggataagc agcgctggag atagcagtcg
     5581 gattgcgaat accggaatgc gagtacgcgt ttgtacattg ggtaaacggt gtcatgtcgc
     5641 cagtaatggt gacctggtgc aaatcgccag ctttggcgca aacgccagaa ttgccaacag
     5701 cggcgacaat gtacacatca ttgccagcgg tgaaaattca acggtagtga gcacgggcgt
     5761 ggtcgactca attattctcg ggccaggggg cagcgccgca ctggcatatc atgacggcga
     5821 aagagttcgc ttcgccgtcg ccattgaagg tgaaaacaat attcgagccg gagtccgcta
     5881 tcgtcttaat gaacagcatc agtttgttga gtgctgatag ctttaccttt aatcaaacga
     5941 aagacgccga cgagcacggg atgcaggctt tacgaccgcc cgagccatcg cctcaccaat
     6001 ttctgcacat gccgtaagtc cctgaacgaa aggctggtcg tgcatcaacc agggcaacgc
     6061 gccgaacgct acgcgcccac gaatatcttc gggttgctgt aacgtataat cttcaccaac
     6121 atcagggatt tcatcccctg ttttctgtaa ttgttcgcgt agtccaggga aaggaatatc
     6181 tttcacttta agcggacgct gcccgcgggc atcaataaaa acgtcaaacg agtagctgtt
     6241 gtcttccgtt ttcaggacgg tgcgcgactc gttaatttcc attttgtagt cttcaccgag
     6301 agcgagaata tgaatgattc ccgcttcacg taaggcaagt aggcgacgaa tagactctga
     6361 agggattgcc gcataattat cgatgaaaac ccgggcaagg cctttactga accgtttatg
     6421 gtcgtgttca ttcagatgtg gaacaatttc ctgtacggct tcatgcaggc gcagaatgac
     6481 ataacgccag ggaacagtat gtttttctcg tttattgcgt tcgacttcct ggagattttt
     6541 ttctgcccag tcaaattggt cgcgttgttt gcgctcggca aaccaggctt gagcaaagga
     6601 atcgacattc aggctctcta aggctatgcg ttgactccag tctggatcag caaacttgat
     6661 ttcctctact atcaatctaa atacccgatc caaaaggcca tattcgcctt tttgaatctc
     6721 agcatttaat gcctgatcag tgacgatgtg taagggctcg tagggaatag ggcaatagaa
     6781 atcggcttcg ggtaaaatac ccgtgcgcga cattaacgta atatttagct tttcacttgc
     6841 gttatcgcgg tgaaaaacga cgtgttgttt atcatcttca atgaacgaac catgctgaat
     6901 agccactgcc attgccgcat ccagtccgct caaggatgtt cccataatac ccacgttaca
     6961 cgcatcgacc tttgcttcca tcaagcctga ccacgggctt ggaaaatacg ttcgggttgc
     7021 ttcttcttca tcaggccaga cgtgacccgt ggcgatcacc gctaaatcaa acgtctcgct
     7081 gggtaaatcc tgatttgtag cgagcatgac gccagcattt gtaatttgca gatcggtaac
     7141 ctggcatgat tcataaacag ccactgcaaa tttttgcttt cgtgcctggt ctactaatcg
     7201 taaaaattga tcgcggaaat attcgcccag cagaattcgc ggtaaaaact gacgatcgtg
     7261 caaggtttct tttttaacgc cataacgctg gagatgactg gcttcttgct tttgtagcca
     7321 ttcgagatac gtacaataaa tcggcggtat ttcaatactg gcaatatttg ccagcatcat
     7381 ttttgagttt tcctcatcac tgtatggcat cccgacaccg gcctcgtcag cctgctcgaa
     7441 gatagaaata gaaagtggag tttgttgctg tagaagcgag aataaggtgt agatccccgt
     7501 aggcccggca cccacaattg caattttttt cattgttact tacctgtacg cattctttcg
     7561 aatttagtgt tatcagataa gtttggttga gatttttaga atttgagaaa tttcagacag
     7621 ggatgaaaag ggcgctaacc gcccttgatt aaattctgtc aggcgagttt tgcctgattg
     7681 atttgactca gtggctcatt gacatcgaaa actttggcga caacttcctc aacgccgagt
     7741 tttttcagtt gggcagtgtg tttttccaga taggcaaggg cgcttttttc atcagtgaaa
     7801 aggtagatcc caccggcttc gtggttcttt tcactttctg tccatacctt ccacagaaaa
     7861 ccaggttcct gattaatcga ctctgcaagt ggtttaagct gctcagccat tgcgtcgcca
     7921 aatgggccat taaaagcaaa atgaagttgt aacaaagttg ccatagcgat tctccttctg
     7981 ataacgcata cgaagacagt gtaaatcttt taacgtattg gatacagaga agaagcagag
     8041 ggaaaaagga gaagaccaga ttaagaaaat ctggtgcgtc cgagtggact cgaaccaccg
     8101 acccccacca tgtcaaggtg gtgctctaac caactgagct acggacgcag gatggtgcgt
     8161 tcaattggac tcgaaccaac gacccccacc atgtcaaggt ggtgctctaa ccaactgagc
     8221 tatgaacgca acgttgtagg tgacaacggg gacgaatatt agcggcagag tgggaaggtg
     8281 gcaagaggca aaacgtaatt ttctgcgcta tttcgaccgt ttgcagagct tttaagcaaa
     8341 ttggctatat tttgttgatt tgcaagggcg atttttattc aggatcgcat ttacatctga
     8401 tacaacccaa taattcgtgt atcagagttt aaacagagtt gtttgccacc atctcaaatc
     8461 tgtcgtggat aaatcctccg tagcctttac acttaggtag caataaaggc acacagatgc
     8521 agcaaaggaa taaatcatgg gatctgatgc gaaaaacttg atgagcgacg ggaatgtgca
     8581 aattgttaag accggcgagg tcattggcgc gacgcaactt actgaaggcg agttgattgt
     8641 tgaagctggc ggaagagccg aaaataccgt ggtcacgggg gctggctggt tgaaagtggc
     8701 aaccggtggg atcgccaaat gcacacagta cggtaacaat ggcacgctat cggtcagcga
     8761 cggtgccatt gccacagata ttgttcagtc cgagggaggc gcaattagtc tctctacgct
     8821 cgctacggtt aatggccgcc atcccgaagg tgaattcagc gttgatcagg gttatgcctg
     8881 cggtttgttg ctggaaaatg gcggtaacct gcgtgtactg gaaggacatc gcgcggaaaa
     8941 aatcattctc gatcaagagg gcggcctgtt ggttaatggg acaacctcag cggtcgtggt
     9001 agatgaaggt ggtgaattgt tggtgtatcc aggtggggaa gccagcaatt gtgagattaa
     9061 tcagggcggc gtttttatgc tggcggggaa agccaatgat acgttgcttg ctgatggcac
     9121 catgaataat ctcggtggtg aagactctga cactattgtt gagaatggag ccatctatcg
     9181 tctggggacg gatggtcttc agctctacag ttccggtaag acgcaaaacc tgtccgttaa
     9241 tgtgggtggt cgggctgaag tgcatgccgg tacgctggaa aatgcggtaa tacaaggtgg
     9301 aacagtgatc ctgttgtcac ccaccagcgc ggacgaaaat tttgtcgtag aggaagatcg
     9361 cgcaccggtt gaactgaccg ggagtgttgc attactggac ggcgcttcaa tgattattgg
     9421 ctatggcgca gatctgcaac aatcaacgat tactgtacag cagggcggtg tattgattct
     9481 cgacggcagt acggtaaaag gtgacagtgt cactttcagt attggtaaca tcaatctgaa
     9541 tggcggaaaa ctgtggctga tcactggtgc ggcaacgcat gtgcagctga aagtgaaacg
     9601 cctgcgcgga gagggagcga tttgcctgca aaccagtgcg aaagaaattt cacctgactt
     9661 catcaatgtg aaaggggaag ttaacgggga tatacgcgtt cagataacag atgccagtcg
     9721 gcaaactctg tgtaacgcac tgaaactaca gccagacgaa gacgggattg gcgcaacgct
     9781 ccagcctgcg taaaaacaca ggcgcacccc gtgaaggttg cgccttgtct ttattagcgg
     9841 gatgctcgtt gcagaatgat ggctgacggc agacgttgca ggtaccgcat acgcagcatc
     9901 atcataatgg ctgccgacgt caggccaata ataaagccta tccagaagcc tgctggcccc
     9961 ataggttcaa cgaccagatc ggtcagtgcc agaatatagc cgcttggcaa gcccagcacc
    10021 cagtaagccg taaaggtgat atagaaaatg gaacgcgtat ctttataacc acgcaaaatc
    10081 ccactgccaa tcacctggat tgagtcagaa atctgatata ccgccgccag caacatcaaa
    10141 tgcgccgcca gcgttacaac ctcgggattg tcgttgtaca acagggcgat ttgctcccgc
    10201 agtgaaaccg tgaatatggc cgtcagggtt gccatacaga cacccaccat aagcccggtc
    10261 cgcgcagcgg tttgcgcatc cagcgttgag ccctgaccca gacgataacc tacgcggata
    10321 gttaccgctg ccgccagcga cattggaagc acgaacatta gtgaactaaa gttcagggca
    10381 atctggtgtc ctgcgacatc aacaataccg agcggagaca ctaacagagc cacgacggca
    10441 aacagtgtca cttcaaagaa tagtgccagc gcaatcggca aaccgagttg aatcagtcgt
    10501 ttcataaccg caggatcggg tttcgcggtg cctttttcgt tacgaatatc gcgcatcgag
    10561 cgggcgcgtt taatgtaaga aaccatggca aggaacatga cccaatacac cgccgcagta
    10621 gccacgccac aaccaacgcc accgagctca ggcataccga aatgaccata aataaagata
    10681 tagttcaccg gaatgttcac cagcaggccg ataaagccca ttaccatacc cggcttggtt
    10741 tttgccagac cttcacattg gttacgggca acctggaaga aaagatagcc cggcgcgccc
    10801 cacagcaacg cacgcagata acccacggct ttgtccgcca gagccggatc gatgttttcc
    10861 atggagcgga taatgtaacc tgcattccac agcaccagca taatgagaac ggaaacaaaa
    10921 cccgccagcc agaagccctg tcgcacctga tgcgcaatgc gctcacgtcg accggaacca
    10981 tttaattgcg cgataaccgg cgttaatgcc agcagcagtc cgtgaccaaa gaggatcgcc
    11041 ggaagccaga tagaagtacc gatagcgacc gccgccatgt cggtggcact atagccgccc
    11101 gccatcacgg tatcgacaaa acccatcgca gtttgggcga tttgcgcgag aatcaccggg
    11161 attgctaatg ctaataacag acgcgcttca ctgatatact tctgcacgtg aacacctttt
    11221 atttgtagtt atatgagaga ctaaaaagcc gcctgaatag gcagcaaaaa gaagaagcag
    11281 gggaaattca gtctattgta gcgaggattt attaattctc cagtgaaaaa atagccattc
    11341 ttaccccttc actggtaagg gcgttttcca cctgctattg tgctgaacag aatgtcttaa
    11401 ctgatttcag gagttgtaag tatgtttacg gggattgtac agggcaccgc aaaactggtg
    11461 tcgattgacg agaaaccaaa ttttcgcaca catgtggtgg agttacccga ccacatgctg
    11521 gacggtctgg aaaccggtgc ctccgtggcg cataacggtt gctgcctgac cgtgacggaa
    11581 attaacggca accatgtcag ttttgacctg atgaaagaaa cgttgcgcat taccaatctt
    11641 ggcgatttaa aagtggggga ttgggtaaac gttgagcgtg cggcgaaatt cagtgatgaa
    11701 attggcggac acttaatgtc aggtcatatt atgaccaccg ctgaagtggc gaaaatatta
    11761 acctcggaaa ataatcgcca gatctggttt aaagtccagg atagtcagtt gatgaaatat
    11821 atcctgtaca aaggatttat tggcatcgac ggtattagcc tgaccgtcgg cgaagtcacg
    11881 ccaacgcgtt tttgcgtcca tttaattccg gaaactctgg aacgcacgac tcttgggaag
    11941 aaaaaacttg gcgcacgcgt caacattgaa atcgatccac aaactcaggc agtggtagat
    12001 acggtagaac gtgtgctggc ggcacgagaa aatgccatga atcaaccagg tacagaagcc
    12061 tgatctgaaa tcgcccccgg agagggggcg atagaatagc ctttagcgag cgactcgaag
    12121 gccgttttca acaccgcgtg agaacaccac ctgccagagc tgaatatcac gggcgcggaa
    12181 agcacctgcg caggcattca gataataggt aaacatccgt ttaaagcgtt cactgtagtt
    12241 atccgcaatt tctggccatg cggcgacgaa tcgttcatac cacgccatca acgtagtatc
    12301 gtaatcagca ccgaagttat gccagtcttc catcacaaag tggggttcgc tggactgagc
    12361 aatctggcgt acagagggca ggcaaccgtt cgggaaaata tatttattaa tccagggatc
    12421 aacattcaga tcggtttttt tcgaaccgat agtatggagc aggaatatgc cttccggttt
    12481 caaattacga tccaccaccg taaaataggt atcgtaattt ttcggtccga cgtgctcgaa
    12541 catccccaca gaaacaatac gatcaaactg gtcgttcagg tcacgataat cttgcagcaa
    12601 aatggtgaca tccaggcctt cacagcgttc ctgagccatt ttttgctgtt cggcagaaat
    12661 ggtgacgccc accacgctta cgtcataatt agatgccatg tagtgtgcca gtccgcccca
    12721 gccgcagcca atatccagta cgcgcatccc aggttttaac tgcaattttt cacaaatcat
    12781 tttgagcttc gcctgctggg cagattccag attatcggca tctttccagt aagcgcagga
    12841 atattgcatg aagggatcaa gcatgcggct gaacaagtca ttacccaaat cgtaatgctc
    12901 tttgccgact atccaggcac gttttttact ctgcagattg aagagacggg cgccggcaat
    12961 acgcagcgtg tctttgaaat gatgggggag ttggttctcg agacctgcgc gtaagacttt
    13021 gctaaaaaac atatccagtc ggtcacattc ccaccagcca tccatataac tttcgcctaa
    13081 ccccaaagag ccttcttgca gaacgcgttt aaaaaaatcg gggtttttca cacgaatatc
    13141 cgccggggca gaaccgttaa tggctatacc ggcacggcta agtaattcgt tggcgatacg
    13201 gtaccagttg tcatccggta cactgacttc ttctatacac gatgaactca tagtttctcc
    13261 atcactggtc ggtgatcaga accttaaaac agcgtagacg ctttttgggc tttgtgagaa
    13321 atctcgcaga aaaaaccgcg agtctataat ccgacgtaga acagagggaa aggaggaacc
    13381 cttgccgaaa tgaccatcca tagtgagtcg ggaacgatcc tgtccccgta aaaacataat
    13441 atttatcgta gctttaacaa ctcgaaatta gtataggccg ctaatttcaa tcatacaaca
    13501 aaattttgtt agcagcctaa gtataagtat atcgatatag atcagtgtga ttcgctatga
    13561 gcgacttcgg cattgccatg attctggcag ccaacttctt cacaacgttg catcatgtaa
    13621 cccagcgcga ccagcactac tgttgataac atcacgctgg tggtggtgag caatggcgtg
    13681 ctgatactga tcagccagga aactaccaga cttgcgagga agcacagacc cagttgcaga
    13741 gtgttctgca acgctgcggc gcgaccagtt gcgtgtggga agggacgcag agcctgggcg
    13801 acaacaatag ggtagatcgc gccattagca atcgccatca cacagaatgg gatcaggatt
    13861 tcgaccagcg acacatggct aatgaagccc gcagcccagg tcgcaatgac gctgacagca
    13921 aacagcacca gcaaccacgg taataactgc ttgccttgcc atttctgcag cgcggcgcga
    13981 cagccataac caccaatcag aaacgcgata gtttgcggga cataacttaa accaataact
    14041 gccgggctgt agcccatttc actaaggatg aacggtgaac cggtcagcca tgcaaaaaaa
    14101 ctggctgaac aggccgcgta tatcagcaca ttgccgcgat aggttttaga acgtagcagg
    14161 tcggtaaagg tcagaccatc ctgactattg ttacgggcct tcgtcgtggg tttgagccag
    14221 aaaataggca gaatcagcac cacggtaatg gcaaaaaggg tggcgaaaat cgcctgccag
    14281 gaaaaatgga ccagcagcca gcttcctaac agaggagcca gggccggaga tagacccacc
    14341 agcggcatga tggtcgcaaa aattcggtta actttctgtg aaggataata atccgtcact
    14401 aacgcttgcc agataaccgc cgcggcgcag acacccacag cctgtacaaa acgcaatacc
    14461 agcagcatag cggcgttttc tacccacagc atccccagac tacctaacgc aaaaattgtc
    14521 aggccgatta ataataccgg tttacgacca taacgatcgg agagcggccc ccacagaagc
    14581 tgggctgcgg caaaaccggc aaggaacaga ctaaggctgg cactgacagc agacgcaggc
    14641 gtttgcaggt cggcctgtat ggcggcgaaa gcaggcagat acatatcggt tgccagaaaa
    14701 ccgagtacgc tcaaacccgc cagccagact aaaaatcttt tcccaggttg catgtatatt
    14761 ctctaaatct ctcaattaaa caccgccgca aagtgtaggg agtgaaatta gtgttgtgaa
    14821 acggtaatat ttgatgactg gtttcaaaaa ttttgcaggc agactatgtg gtcagaatat
    14881 tcactcgaag ttgttgatgc ggtagcgcgt aatggtagtt ttagcgctgc ggcacaggag
    14941 ctgcatcgcg ttccttctgc ggtcagctat accgtgcgtc agctggaaga gtggctggcg
    15001 gtgccgctgt ttgaacgacg gcaccgtgat gtggagctga ccgctgccgg agcgtggttt
    15061 ctcaaagaag ggcgctctgt tgtcaaaaaa atgcagatca cccgccagca atgtcagcag
    15121 atagcgaacg gctggcgcgg tcagttagct atcgcagtgg ataatattgt caggccagaa
    15181 cgtacacggc agatgatcgt tgatttttat cgccattttg atgatgtcga acttcttgtc
    15241 tttcaggaag tgttcaacgg tgtctgggat gcgctttccg acgggcgcgt ggaactggcg
    15301 attggcgcaa cacgcgcgat tccggtaggc ggtcgttatg ccttccggga tatggggatg
    15361 ctaagctgga gttgcgttgt tgccagccat cacccgctgg cgttgatgga tggcccgttc
    15421 agcgatgata cgttgcgcaa ctggccgtcg ttggtgcgcg aagacacctc gcgaacgcta
    15481 cctaagcgta ttacctggtt gctggataac caaaaaagag ttgtcgtgcc tgactgggaa
    15541 tcatcggcaa cctgtatctc ggcaggatta tgcataggga tggtgccaac acatttcgcc
    15601 aaaccgtggc ttaatgaggg gaagtgggta gcattagaac tggaaaaccc ctttccggat
    15661 tcggcatgtt gtctgacatg gcagcaaaat gatatgtcgc ctgcgttaac ctggttgctt
    15721 gagtatctcg gcgatagtga gacgctgaat aaggagtggc tgcgggagcc ggaagagact
    15781 cccgcaacgg gtgattaacg acgatagtcg cggaacgggc cgtcagccac ggagcggcgt
    15841 tcaatcaagc gcggatgcac ttcaatggac tgcggttctt cacgtttgtt gactatacga
    15901 tccaacaaca tgttgaacgc tgtttcaccc aacgaatctt ttggctgatg gattgttgtc
    15961 agcgccggcg taaaatagcg ggcgttgcgc acgttatcat aaccgatcag cgaaacatcc
    16021 tgcgggacgc gcagccccat ttcatcagca gcacaaagtg cgcccattgc catgatatcg
    16081 ccaccacaga agacggcagt agggcgatgc ggctgcgaca ggatttgctg catggcgcga
    16141 taaccggatt caggttcaaa gtcaccctgc acaatccagc tttccggcac cttgatcatt
    16201 gcttcttcca tcgccttcat aaaaccggca aggcggcctg cgccagtgtt acgttccagc
    16261 gggccgggga taacgccgat ttcgcggtga ccgcgttcaa tcagataacg cccggccatg
    16321 tagccgcctt cgaacgcgtt atcaatgacc gcatcggtga agtcagcttt tgcttcaccc
    16381 cagtccatca ccaccattgg gatatggcga tactcttcca gcatcgccag caacggctct
    16441 gggtactcag aacacatcac cagcagacca tcgacgcgtt tttgcgccat catcgacaga
    16501 taagcccgct gtttctcaag attgttccac gcattaccca gaatcagggt gtaacctttc
    16561 tggaagcaat ttttttcaac tgcttcaatg atctcggcaa aataggccgc ttcgctgctg
    16621 gtcgctagca aaccgataga cttggtgtgg ttaaccttca ggctacgcgc caccgcgcta
    16681 ggggagtagt gtaattcttt gatcgctgcc cacacggcgt tgcgcgtttc ttcagcgacg
    16741 aaacgtgttt tgttgatcac gtgtgacaca gttgtagtgg aaacgtttgc tcgtttcgct
    16801 acatctttta ttgttgccat tccatttcac tccagaccct aacttcagct cctgcaaaat
    16861 cgcaaggtaa acgtttgcct ttacacacct tacgcacaac aaaagtgttg cggtacgccg
    16921 gaaaaacgac acggagcgtc aggaaggggt caatggccag tacgcaaata gtgtaagcgt
    16981 ggaattttgg ccgatcttga cgaaaagggg aagtggtaaa aacacttatc attataaatg
    17041 acgggagatt ttttcatcac agtgtgtaaa aatgcgtaat atcacttttt gtgtggtaat
    17101 aaaaaaggag aaaacttgat gagcaccgac cttaaatttt cactggtaac aacgattatc
    17161 gtcctcggtt tgatcgtggc cgtgggtttg actgccgcgc tgcactgatt tcctctcgag
    17221 ggagcgcgtt ctccctcgca tctccttcct gtttgttagc aaaatggcaa aagtgttttc
    17281 tctttttgtt attttcattt ttttgtgatt gctgtcatgc tttcgccact ttaatgaata
    17341 tcgcgtaatg acgcgaagag gtggagataa ggcatgaaaa ttaactatcc gttgctggcg
    17401 ctggcgattg gcgcgtttgg tatcgggaca acggagttct cgccaatggg cttgttgccc
    17461 gtcattgcgc gcggtgtgga tgtctcgatt cccgctgccg gaatgttaat cagtgcctat
    17521 gcagttggcg taatggttgg cgcgccgctg atgacgcttc tactttctca tcgtgcccgc
    17581 cgcagtgcgt tgattttcct gatggcaatt ttcacgctcg gcaacgtact ttccgccatc
    17641 gcgccggatt atatgaccct gatgctttca cgcattttga ccagcctgaa tcacggagca
    17701 ttttttggtt tgggttcagt cgtggccgca agcgtggtgc caaaacataa acaggccagc
    17761 gcagttgcca ctatgtttat ggggttaacc ctggcaaata tcggtggcgt gccggcggcg
    17821 acctggttgg gtgaaaccat cggctggcgg atgtcatttc tggcaacggc ggggctggga
    17881 gtgatttcaa tggtaagtct gttcttctca ttacctaaag gtggtgcagg ggcacgacct
    17941 gaagtgaaaa aagagctggc ggtattaatg cgtccgcagg tgctgtctgc attgctgacg
    18001 acggtactgg gagctggtgc aatgtttact ctctacacct atatctctcc ggtactgcaa
    18061 agtattaccc acgcaacacc ggtgttcgtc acggcaatgc tggtgctgat tggtgtcgga
    18121 ttctctatcg gtaactatct cggcggcaaa ctggcagatc gttcagttaa cggcacgttg
    18181 aaaggctttt tgttgctgct gatggtgatt atgctggcaa tcccgttcct ggcccgcaat
    18241 aagttcggcg cagctattag catggcggtg tggggcgctg caacctttgc ggtcgtaccg
    18301 ccgttacaga tgcgcgtgat gcgtgtcgcc agtgaagcgc caggtctgtc ttcatcagtc
    18361 aatattggtg cctttaatct tggaaatgcg ctgggagcag ctgctggtgg tgcggtaatt
    18421 tccgctgggc tgggatacag ctttgtgccg gtgatggggg cgattgtcgc gggactggca
    18481 ttattgctgg tgtttatgtc agccagaaaa caacctgaaa cagtttgcgt tgctaacagc
    18541 taattaaaca aaaacgcaga aggataacct tctgcgttac agttgctgac aacgtgcgat
    18601 ttatcttgcc gaatgcgctg ttaacgcctc atccgcgttg gcatggtaag cgaagcgcat
    18661 caggcaatac tgcatttgcc atcagttatt atgcagcgag atttttcgct acgaattccc
    18721 agttcaccag cgcccagaag tgctcaagat agccaggacg tgcattgcga tagtcgatgt
    18781 aatatgcgtg ttcccagaca tcaacggtta gcagcggagt cgcatcggtg gtcagcggag
    18841 tacccgcgtt agaggttgaa acgatagcca gtttgccatc gctgtttttc accagccagg
    18901 tccagccaga accaaagttt ttgatcgctg catcagtaaa ctgagctttg aaatcggcaa
    18961 agctgccaaa agatgcggcg atagcttcag cgacttttcc agtcggttcg ccaccggcgt
    19021 tcggtgccag gcagttccag tagaaagtat ggttccagac ctgagctgcg ttgttgaata
    19081 cgccaccttc agagctgcga ataatctctt ccagtgattt accttcaaac gcggtacctt
    19141 taatcaggtt gttcaggtta gtgacataag tctgatgatg cttgccgtag tgatactcga
    19201 tagtttccgc agaaatgtgc ggtgccagag catctttagc atatggtagt gcaggtaatt
    19261 cgaatgacat tgctactctc ctttattatt aatttgcata cgtacaatag ccttattgtg
    19321 cgtatggata gggtagcaaa ttaaaaggtg agacaaaaga tgaacttacc ctgttgccgt
    19381 aacaacaggg taaagtttta gcgaagtgtt tttggggtca ttacccggcg agcgccaaca
    19441 tagtggcgct gccagtagtc ttcactgaga gaagtgattt gaatttcctg acctgtgcgc
    19501 ggtgactgaa taaatttgcc gttgccgaca tacacgccga catgatcggc tgtaccgcgt
    19561 ccctgagtac ggaaaaagac caggtcgccg tttttcagtt cactacgttc gattggcgct
    19621 gcatcgcgca ggtgatacat ttcattcgcc gtacgcggaa tacgaatttt caccaaatct
    19681 ttataagcgt aataaaccag gccgctgcaa tcaaaaccgg tacgcggtga gctgccaccc
    19741 caacgatatg gcttaccaat ttgctgcatc agtttattca ttgccacttt tgtggctttt
    19801 tgcaccttcg ctttgtgtgc gtcggcaagg gttcctgacg ctgcattttt gactttcaca
    19861 caatgcgatt tatagccctt acgtttggta cacttttcgg taaagctggc tgaggccgtt
    19921 ttattgacgg tatgtttgct gcgagttttg gcgtttttag aagcggtaat ggaagatttt
    19981 ttaacggaag aagcggtttt gcttttggtc gtacttttac tggcggcttt tttcgctgtt
    20041 ttctgggtct ttttggtggt tgccgtgctt ttctttttat ctgctttttt ggtgatatga
    20101 gtggtagcag aactctccct ggcttgcttt gaagcatggg ccataggcgt taaaggcagg
    20161 gtggtaaaaa gtaaagcaca gagcgtgatc gaaatacggt ttatccgcgc cactgagcgt
    20221 ccctctgtta aggcaggtct atcatgcctg cgtctgattt acaaaacttg atgattctaa
    20281 tgcatagaag aagagaaagt taatagcttt tattgttaaa aaatacgttt cgttggcgag
    20341 cgcaaaaaaa tgcagcagtg aaagctaaaa atggtcataa atacgccagt ttgccgtgca
    20401 tcagggaaga aacgaatatt tgaaatgcaa caaatagagc aaagggataa aatagcaaaa
    20461 gcgtgatgaa aatgttattt tccgatgccg cactgacccg ctacaatgtc agattactgc
    20521 cgtaaagaag tttgaggaag caagacaatg agcaccacta tcgaaaaaat ccaacgccag
    20581 attgctgaaa acccgatcct gctgtacatg aaaggttcac cgaaactgcc gagctgcggt
    20641 ttctctgccc aggcagtcca ggcgcttgcc gcatgtggcg aacgttttgc ctatgttgat
    20701 attctgcaga atccggacat tcgtgcggag ctgccgaaat atgctaactg gccgaccttc
    20761 ccgcaactgt gggttgacgg cgagctggta ggcggttgtg atatcgtgat cgaaatgtat
    20821 cagcgtggcg aactgcagca gctgatcaaa gaaactgccg ctaaatacaa gtctgaagag
    20881 ccggacgcgg aataatccgt cctgcaatac tgacggatgc gaacgggcat ccgtcagtcc
    20941 tttctctatc cccaatccag cccttgtggc gaacttgaaa atcccgcttc acgcagcagc
    21001 gtaaacatcg gcgtttgcca gaccggtaga tcattcactt ctgttagcgt aaagcgcagc
    21061 cgtggttcgc gacgcagtgc ggtagtcagc gcgtggaaaa cctctggggc gagtaattcc
    21121 tctttttcct gccagaccag catttttttg ccaccttgcg ccagatagag taacaatttg
    21181 ccgccagaaa cgaccaccag cgcacccgcc cgacgcgttg gtaccagcgt tgccggatgt
    21241 gcaggccagg gcagaagatt tccccacaca ttagccggat cgttggcgga gagcgccact
    21301 ggtgtatagt ggcgcgtttg cgcggcttgt gtcgccaggt cgcgcaagcg gtcaatagtc
    21361 agacgttcag cgaattgcgc gccacccaga ccttctacaa aacgacctcg cataattcgc
    21421 ccggaatctt ccatacttcg acaaagcgtt tgcatcgatg gaaacccgcc agggatattt
    21481 tcggctatca ccgcctgacg actgatgatg ccgtagcggt cgagcatatt ttccgccagc
    21541 gccagcatcc tttcggtatc gtttagtggc tccacctgca ataacgacca gcgtccagcc
    21601 agatttggtg tgttgtaaga tacccgcggc gagacgggtt gtgcatagac aggacgccca
    21661 cgacgagccc ggtgactgcg gcgagttgag gtgcgtgcgt tggaactgct gcgggtgagg
    21721 gcgcgtaacg gtgcccaaat atcgctggtg atgacgcctt gccagaccag cgcccataac
    21781 gcctcttgca gtgccgaggg atcaaccgat tcgccgattt tgtcgcgtat ccgttggctg
    21841 atttgttgtg caaaccaggc tcctccgtca gccagaacag cgactatcgc ttgttgcagc
    21901 gccgaacgat tcgcctgatc cgcttcggcg ggagtgaacg attctgcagc atattcctgt
    21961 agatgcagtg ccaccaggcc gtcatcttca cccagttttt tttgccccga ccagataacc
    22021 gcaccggttg ccagtaattc atcgagcact tctggcgaat agtcgtgtac gcgggcaggc
    22081 aagatctggc tttcccagag tgaggcgggt aagccgactc cggcaagctg ttcgatcacc
    22141 cgcatcacgc catccacccc ctcataaacg cctggcgatg ttgaggcaaa gagcgccggg
    22201 ctaccatcgg tggcgggtaa tacgccctga cgttccagca gcaatcgcgc ataggtcgtg
    22261 gctgcaacgg gacgcgtcgc ttctctggcg gcttgcagcg agcgcaaacg cagacgacga
    22321 aatacttcat cgctcaccca gatgtcttgt tgcagattca tcaccagacc ctgttcgcgc
    22381 agttgctgaa gctgctcttc gacaatggca ataccgagac taaactcatg agccagttgt
    22441 tcagccgtga ccagagcatg agcccggaga tagcgtagaa agaggtcgcg aagcgggtaa
    22501 ctgactctat gcaaataaat ctctggcagt gactctggta atcgtacgcc gagggcatcg
    22561 cgcagcctgg cggcatcatc catacaggca agacgctcct gtccactaat catcgtcggg
    22621 aagatgcgtt ttactgtaag aagattttcc agatacgagg caatctcttc actgctgcct
    22681 gtatgccgtt gcgccaggtc ttcaacggtc attggcccca gttcgcgcag caggtcgaac
    22741 aatccttctt cacctttcgc tcttctgcca ggagccagtc gttgcaactc ttcttccacc
    22801 tggcgaatga cctgcgggtc gagtaattcc cccggatcga cctgtccgag tagattgcgc
    22861 agtaactcgc tgtccagcga cagtacggat gcccggcgtt ctgccagcgg ggcgtcgctc
    22921 tggtacataa attccgcgac atagccgaac aataaacttg tggcgaaagg cgagggcgta
    22981 gtagtcgtta catcggatat ttgaatttcg cctccgttca ggcgacgcat caaacgttcc
    23041 agtgcgggaa gatcataaac atcttgcaga cattcgcgta gggtttcgag aatggccgga
    23101 aaatccggat atccctgagc gatttccagc aactgactgg cgcgcagccg ttgttgccaa
    23161 agcggggtgc gatggcccgg cgtgcggccc ggcattaata atgcccgcgc ggcgcattcg
    23221 cgaaaacggg cggcgaaaag tgccgaactg cctaccgcct cgcggacaat ttgcagcaac
    23281 ttttctggtt caaacaaaaa aatcgcggcg tcgggcaatt taccatcagt gtcaggaatg
    23341 cgcgcaacaa tgccgtcatc actggcgacc acagacgcgt cagcgcccca aagcgcatgt
    23401 attcgcccgg caatcgccag cgcccagggt tcatgcaccc gtcttccata gggagagtgc
    23461 aaaataatac gccagtcgcc aatttcatca cggcaccgtt cgaggaccag atgacgactc
    23521 cccggaacaa tacccgtcgc gttgcgctgc tcgtcaatca acccctgaat attggcgatc
    23581 gtattttctt cagccagcca cgggggaata gtcccggaaa agaacgctcc atccgccagc
    23641 aaatgaagaa aatcgccgat catctcgcct aattcagccg gacgtccgtt accttcacca
    23701 cgccagaagg ggagccgggc agaacgaccc ggagcaggag tcacaatcac ctgatcgcgg
    23761 gtgatttgct ggatccgcca tgaggttgcg ccgagagtga taatatcgtt cacccgcgac
    23821 tcatatacca tctcctcatc cagttcaccc acccgtcgcg aaccggcctg ttcttcccct
    23881 tcgggtaata acacgctata catgccacga tccggaatgg tgccgccgct ggtaaccgcc
    23941 aacagctgag cgccaggtcg ggcggtcaat atcccggtct ccctgttcca gaccagtttg
    24001 gggcgaaaag cagaaaaatc gccagaggga tagcgtccgg aaagcatatc cagcgtggcg
    24061 tcaaaaacac gtcttggcag atctttccac ggtgcggcac ggcgtacgcg ggagtaccat
    24121 tcgtctacct gtaatgcatc catcgccgcg gcggcaacgg tgtgctgcgc aaggacgtcg
    24181 agtggattat gcggaggtgt caggttttcc agcctgcctg cgaacataca ctctacaatg
    24241 actgcggaat cgactaagtc acgcctggtt cgggggaaaa aaagcccttt ggagatacct
    24301 cctatctggt gtccggcacg tccaatgcgt tgtaacccac tggcaacaga aagcggcgtt
    24361 gccacctgaa tcaccagctc cacagcgccc atatcaatcc ccagttcgag actggaagtt
    24421 gcgaccacgc agcgtaactc acccgatttc agcgcctgct cggtgattgc tcgttgttct
    24481 ttagagacgg agccgtggtg tgagcgggca ataaaaacgt cgctactttg tacacggtta
    24541 gaggttgcgc cagaggtcga ctcgaaatgg gccgcatcag cggcgataga cggggaacgc
    24601 tgtaagcgtg cggcgtaaag ctcatttaat cgtgccgtca gtttttccgc cagcccccgc
    24661 gaattagtaa agacaatggt cgagcgatgg cgcaacactt catcaaggat acccgtttca
    24721 atatatggcc agatggagcc ttcccggccg gcatggctgt cttcgccagt gccgctggcg
    24781 accgatgaga catcatccat attggcgact ggtacgacaa tccgtatctg cggatgacgc
    24841 attgcgggcg ggttgactac cgtaaccggg cgatcgccac caagaaatgc tgccacatcg
    24901 ctggctgagc gcacagtggc agaaaggcca attcgctgtg ctgaggtgtg gagcagcgca
    24961 tcgagccgct ccagacttaa cgccagatgc gcaccacgtt tacttcccgc taccgcgtgg
    25021 acttcatcaa taattaccgt ttcgacgccg cgtagcgttt cgcgcgcgcg ggaggtcagc
    25081 atcagataga gtgattcggg tgtggtgatc agaatatccg gcggattacg ggtgagtttg
    25141 ctgcgttcct gtgcaggcgt atcgccagta cggatcccca cgcgaagatt gacttccgtt
    25201 tcgccgcgcc gccgccgttc atcggcaata cccttcaacg ggatctgcaa gttgcgctga
    25261 acgtcggtgc ccagggcttt tatcggtgaa atatagagga tgcgtgaggt tttacgttta
    25321 tgcgcctcgc gggtatcttc gccgccttcg cggaagagtc gatcgagggc gtagagaaat
    25381 gctgccagcg ttttcccgga gccagtcggt gcaatcacca gcgcatgttc gcctcgcgcc
    25441 gccacatgcc aggtttgcga ctggacagcg gtcggctgtt taaaggcgcg aagaaaccag
    25501 tcgcgggtcg ccggtgaaaa cacgtccggc aggggcgatg aagggtttgg attatctgcc
    25561 attaacgcta ttccccgtga atatcatgca tgatgcgtgg aatagcctca ctatagatgc
    25621 ctgaatcatt gcatgtcatc aggcatcgac tcgattacac ctcttcggcg gcaggtagcg
    25681 gccagcctcc cagacgtttc cagcggttga cgatttcaca aaacagcacc gcagtgcgtt
    25741 cggtgtcgta cagcgcggag tgcgcctggg tgctgtcgaa gtccatgcca gcggtctggc
    25801 aagcctttga caatacggtt tgtccgagtg ccagcccggc cagtgcagca gtgtcaaaag
    25861 tggcgaaagg gtggaacggg ttacgtttca gtgaggcgcg ttctgcggcg gccatcataa
    25921 agctgtgatc gaaattggcg ttgtgcgcca ccataatggc gcggttacag ccgctcgctt
    25981 taataccttt acgtacaact ttaaaaattt cgtgcagcgc ctcgtattcg ctgaccgcgc
    26041 cgcgatcggg atcgttcggg tcaatgccgt tgaaagcaag ggcttctggt tgcaaatttg
    26101 cgccgacaaa tggttcgacg tggaaatgta atgtggtgtc cggcatcagc cagccttgtt
    26161 catccatttt cagggtgatg gcggcaatct caagcagcgc atcggttttg gcgttaaatc
    26221 cagctgtttc aacatcgatc acaacaggat aaaagccacg aaaacggtcg cacagaccgg
    26281 taagttgagc gttatcggac atctgattct cttaatacgg ataaattgca gcgcgcatta
    26341 tgacaaatat tacttgtcga tgcagtaaag atgcgggcgc gatgagttcg cgcccggcag
    26401 gagattagtt gcccagaccg cgaccggcgt ctttctcttc gattaactca attttgtaac
    26461 cgtccggatc ttccacaaac gcgataaccg tagtaccgcc ttttaccgga cccgcttcac
    26521 gggtcacgtt acccccgttt tgacggattt tttcgcacgc ttcagcggca ttatctacgc
    26581 taagcgcgat gtgaccataa gcagtgccga gttcgtattt atccacgccc cagttgtagg
    26641 tcagttcaat caccgcttct tcggtttccg ggccgtagcc aacaaacgcc agtgagtatt
    26701 tgtattccgg gttttcgctg gtacgcagca gtttcatgcc cagcactttg gtataaaaat
    26761 cgatggagcg ttgcagatcg ccaacgcgca gcatggtatg aagaagacgc atctttttat
    26821 cctcaaaatg gctaacagat tcaaaatgtt gttttagtat agcggcggct ttacgccgct
    26881 cgcaatgttg gattacaacg tcgggtaatc agtgtagcct tccgcaccgc caccatagaa
    26941 actttcagca cgctgtgggt taagctcagc tttgcgctgc aagcgggcga ccagatccgg
    27001 gttcgcaatc cagtcacgac caaatgccac cgcatcaatt aaccctttgc cgatcagcgt
    27061 ttcagctttt tctactgtgt atgcacctgc gccgataatc ggaccgtgga aacgggcgcg
    27121 tactttttcg cggaacgcat cagtatacgg ttcacccccc gcccaatctg gttctgacat
    27181 atgcagataa gcaatgccgc gtttacccag ttgttcaatc agatacagtg catcggcttc
    27241 ttcattcggg ccgttatccg tgttctggaa agtaccgatt ggcgaaacgc gaatgccaat
    27301 gcgatcggca ccccattctt caatcccggc atcgaccact tccagtacca aacgtgcgcg
    27361 attttccacg ctgccgccgt actgatcggt acgatggttt gaagaaggag aaaggaactg
    27421 atgcagcaaa taaccatgag cagagtggag ctctaccaga tcaaaaccgg cttcacgcgc
    27481 gttagcaata gcctgacgga aatcattgac gatacttgga atctcttcca gttcaagcgc
    27541 acgcggcatg gatgtttcaa cacggatcgc ctgaccattt tcatcgcgca gagaagtacg
    27601 tgttcccgcg ctaagtgctg aaggcgctac tggtgcctgg ccgccaggtt gcaggctggc
    27661 gtgagaaatg cgtccggtgt gccacagctg tacggccata tgcccatttt cagcatgaac
    27721 gccagcggta atttttttcc atgcggcaat ttgttccgga ctatggatgc caggtgcacc
    27781 agcataccct tttgcctggg cagaaatttg cgtggcttcg ctaataatca aaccggcact
    27841 ggcacgttgg cgatagtatt ccgccatcaa cggggtaggg atgtcacccg gttcaatact
    27901 gcgcagacgc gtcagcggtg ccataaaaat acggtttgcc gcagttattg cgcccacttt
    27961 cagtggggaa tacagttttt cagatgacat aaaggctccc ggtcgtctgg tgataaaaaa
    28021 tgcctgctaa acggcaggcg tcgcaataat ggtttttaca tgtgccagcg cgttttccag
    28081 tggctcggaa ttgcgcgaaa ttttggcctg cagattcgca cccagccata gtgcgtaaag
    28141 cacttgtgcc tgttgcagcg gttcgccaca aaaggttaaa caatggctat cacggccatt
    28201 ttccagagcc tgcgagagca gggcgatcac gccgcgagcg cctttatcca tcgcgctacg
    28261 catatcttct gacagatcgc acacttcggc agagagtttt actgtcaggc aaccactgat
    28321 ggttccatgt tggcaaaact ggttcagtgt ttgctggtaa taagccagta tgcggtcgcg
    28381 gtagttacct tcgccggatt gcagcaactc agtcagtcgt tggtgatatg tggcgtaatg
    28441 gcgctcaagc atggcaacgc caaacgcttc tttagagcga aagtagtgat agaaggaccc
    28501 tttcggcact tcagcggttt ttagtaattc gcttagcccc atcccggtga atccacgttg
    28561 caggcaaagt tgctcgcccg tcgccaggag atgttcgcga gtatcatgtt cggtgtgttt
    28621 gttcatgcgt tggagtgtag tagaccagtc ggtctagtgc aagcaaagtt gctactttgg
    28681 gcattaacag cttcaataat ggtaataatc ttatttgagg agggcgaagt ggcggagcaa
    28741 ttagagttct ttcccgtcca gagcccgtgc cggggaattt gccagtctga cgaacgcggg
    28801 ttttgtcgcg gctgttttcg aagccgggat gagcgtttta actggaataa aatgagcgat
    28861 ggcgaaaaac aagaggtgct gcgcctttgt cggcagcgat taatgcgcaa attacgtgca
    28921 aataagccag cgtcatcaga cgaaccggag caaccatcac tcttttgagc ctaatcttgc
    28981 gtatactcct ctcatatctg ataagaggaa gcgattatgg ttcagcgtat tactattgcg
    29041 ccgcaaggcc cggagttttc ccgttttgtg atgggctact ggcgattgat ggactggaac
    29101 atgtccgccc gccagctggt cagttttatt gaagagcatc tggatctcgg cgtgaccacc
    29161 gtggaccatg ctgatattta tggtggctat cagtgcgaag cggcgtttgg cgaggcgctg
    29221 aaactggcac ctcacctgcg tgagcggatg gaaatcgtca gtaaatgcgg tatcgcgacg
    29281 accgcgcgtg aagaaaacgt cattggtcat tacatcactg accgcgatca catcattaag
    29341 agcgccgaac agtcgctaat taatcttgcg accgatcatc tggatctgct gttaatccac
    29401 cgcccggacc cgttaatgga tgccgatgaa gtggcggacg cgttcaaaca tctgcatcag
    29461 agcggcaaag tgcgtcattt tggcgtatcc aacttcacgc ctgcgcaatt tgccctgttg
    29521 caatcacgtc tgccgtttac ccttgccact aatcaggtgg aaatatcccc ggtacatcag
    29581 ccgttactgc tggatggcac gctcgaccaa ctgcaacaac tgcgtgttcg tccgatggcg
    29641 tggtcctgcc ttggtggtgg tcgtctgttt aatgatgatt atttccagcc gctgcgtgat
    29701 gaactggctg tggtggcgga ggagttaaac gcgggctcga ttgaacaggt ggtttacgcc
    29761 tgggtattac gtttaccgtc gcagccgctg ccaattatcg gctgaggtaa aattgagcgc
    29821 gtacgggcag ctgtcgaagc agaaacactg aaaatgaccc gtcaacaatg gtttcgtatc
    29881 cgtaaagcgg cactggggta cgacgtaccg taagcacttt taggaatagc cgccgttcaa
    29941 aaatgtgtca ctggtttaca cttattcagg aatgcacaat gaacggaggt cctatgaaac
    30001 gttttagtct ggccattctg gcgctggttg ttgcgaccgg cgcacaagct gccagtgaaa
    30061 aagtcgagat gaacctcgtc acgtcgcaag gggttgggca gtcaattggt agcgtcacca
    30121 ttactgaaac cgataaaggt ctggagtttt cgcccgatct gaaagcatta cctcccggtg
    30181 aacatggctt ccatattcat gccaaaggaa gctgccagcc agccaccaaa gatggcaaag
    30241 ccagcgccgc ggaatccgca ggcgggcatc ttgatccaca aaataccggt aaacatgaag
    30301 ggccagaagg tgccgggcat ttaggcgatc tgcctgcact ggtcgtcaat aatgacggca
    30361 aagctaccga tgccgtcatc gcgcctcgtc tgaaatcact ggatgaaatc aaagacaaag
    30421 cgctgatggt ccacgttggc ggcgataata tgtccgatca acctaaaccg ctgggcggtg
    30481 gcggtgaacg ctatgcctgt ggtgtaatta agtaattaag aggccagcgt accttgcggt
    30541 ggtgcttgct caagttgcga aagcgagcag tacagccgcc agacaattgc cgccagctca
    30601 cgggcggcag gttgatgatg gcgggcaagg ctgtcgcaaa tccgctgtaa ttcttcaagt
    30661 gtggccgcca gtgatttttg ctgaacgcca cgctcactca tcactccccg caacagtgaa
    30721 atacagttat cccgtactcg cgataacgga tcggaacgcg attcccagtc gcgtaattgc
    30781 cagacaacat gagaacagtt cagcagcact ataccccagc gtaataacca acggcgagcc
    30841 agcgcatcct ggctgttact caactgactg acgtgatgat aagtgagcga ttcaaattcg
    30901 ctttcactca gtgttggatg gcggcttagt tgatcgacaa aatcccggcg cagcgcgcga
    30961 atatggcggc ggcttttgcg agcatccgat cctggacgca gaatggcgaa cgctaaccac
    31021 gccaacgcga cgccaacgat ttttgccaga ttatcgttaa gaaaatcagc aaaattatac
    31081 accgggggat tagtgacggc gataaaagaa cccataaaaa caatcagttg cccccacaat
    31141 gcggcaaatt tgggcatctg caatttaaga agctgcattg tcgccagcag tggaaagaga
    31201 aacagtaaaa attgccacag atcgctaatc tggaccatca gaccaaattt gaccacaaag
    31261 ctgaatagcg aaagtaacac cagcgtgcgc atcagcagtg acaacgactt aaacggtgct
    31321 gcgacggcgg agtagagtac gcagctaatc gctgccagcg ttaatgcatt ggcaccggca
    31381 tcccattgcg aagcaatact ccatgcgcca atcatcatca acgtacaaaa tgtacgcagt
    31441 ccgctccaca tagcttcggc gttatcggta tgacgagcca ggccggatgt ccgtgggagt
    31501 ctggcatgat catctacatc gctttgcaga cgatgtaatt cctgactact ttgcagataa
    31561 aggcggcaaa aataacgtag tcgctgccag aaggcgacgt gccgatagtc ggcgacgttg
    31621 gtcgggcgta gcggggcgat aatacgtgcg acggtgtaaa catctgtttg cgaactggcg
    31681 agcgccgtca gcaactgttc gagaatttct cgcgtggcac ctggcggtga gggccagttg
    31741 agcaacatac gtcgcaggct ggagatgaca ctggtcatac gtaattgctg gtggagcagc
    31801 gcattaaggc gcgcgttttg ctggcgaaaa cgatagtggc tccagaaagc ctggatacgc
    31861 agcaaattca tggtcagtat ctgcccaatc accccttcat gtgctgcacg aatggcatcg
    31921 gttgtttcag gctgccagag taaactggca tgttccagta atcgggcgtg catgtttttc
    31981 aatgcggtta aaagggctgt agcatcggaa ctgctaggca ggatcatcat catcatgccg
    32041 ccgcacaaaa taccgacaat cacctcgcaa acgcgcgcct gagcgatatc ccacagctgg
    32101 ctggcctcag taatattaac catcggaaag gcgatgatgg cagccgtgta gcccgccagt
    32161 tgaaatgcat acgcgacgtt attcgtgaag tgcgcacagg cccaggtaca aaagccaagc
    32221 cacgccgaca tgctcaatag aaaaaaccac ggctcattga gcgtatgccc ggcaagaagc
    32281 agtgccgcaa tggctcccag taaactgcca gcgatgcgtc cgaggctttt gctgatgaca
    32341 ccgccaacgg tgggaaagct aaccactgca gccgaggtca tcgcccaata gggttcatcc
    32401 agattcaaat aataggcaac cgtcagcgcc agacacatgg caatggtatt gcgtaacgca
    32461 taacgccatt gcgccagcgt ggccctgaac cagggcaaat tgcgcaagga ccatgacgat
    32521 gcgttcatta ttgacctacg gcaatgctac aggtggtgcc cgacaccagc gtgacatcgc
    32581 ctggaacttt atccagcgca aagcggacgg gaacgcgttg ggcgagacga acccagggga
    32641 cgttaggttt cacatccgga ataaggcttg agtcactttc aacgctctga tcataaatcg
    32701 cccgaccgat actggaaacg tgaccctgta acgttttgtt atcactatag agggtaattt
    32761 gtgctggtgc accttcgcgg atatggcgca acttggtttc ttcaaaataa ccaatgacgt
    32821 aaaacgagtg gctgtcgaca agggcaaaca gcggtttccc ggtgtcagcg taatcgccga
    32881 tgcgggtagt gagattagtc acccatccac tcaccggggc gcgaatttcc gtttgcgcca
    32941 gttgccattg tgtctgtttc agcgtcgcct gtgcggcatc aacactggcc tgcatcgctt
    33001 taacattgag gttagcggta tcgagttctt cggcagaaat aaaattttgt gagagatgac
    33061 gacgacgatt cgcttcgtta ttggcttttg cgaggtcaga ttgcgctttc gccagctgag
    33121 cctgtgcatt cagctcggcg atctgaaagg gcgttttatc gatcgtgagc agaagatccc
    33181 ccgcgttaac cagctggttg tcttttatat tcagctcaac aatacgtccc gacacctgtg
    33241 gggtgataga aacctgttct gcgcgtattt tcccgtcgcg tgtccacgga gattgcatgt
    33301 aataattcca cagccaccat ccggcaagaa ccgcaactac cgctacaatg attgttgaga
    33361 aatacttaat tgttttaatt gacatagtta ccacgcaatc agtattgcca gagcaaggca
    33421 aacgcaaatc gcaaacagcg ataaatccat taacaaggga tgccagatgt caccggcgta
    33481 gatccagcca cgaagcaggc ggtgtacgac aagccagatg acgaatccaa acgcgaaggc
    33541 tttgaaaaac ggaggaaagt agacggacgc accgaacacc agatcttgta agggcaatcc
    33601 tgttgcattg agcataaact tcacaaagta gattccttta cgaccgaata tattgcgtta
    33661 gattaataaa tattctttaa ctgcgaaaaa tttacgcgca atttctgaaa aatatgtctg
    33721 acggtaacca aatgcagcaa tacatttgtt ttagcaatac aattgctgca cactattcta
    33781 aaagccgcat aatatcttag caagctaatt ataaggagat gaaattggaa tcgccactag
    33841 gttctgatct ggcacggttg gtgcgcatat ggcgtgctct gatagaccat cgcctgaaac
    33901 cgctggagtt aacacaaacc cattgggtta cgttacacaa tatccatcag ttacctccag
    33961 accagtcgca aattcaactg gcaaaagcga ttggcatcga gcagccatca ctggtccgta
    34021 ctctggacca actggaagaa aaagggttaa tttcgcgtca aacttgtgcc agcgatcgtc
    34081 gggctaaacg tattaaactg acggaaaagg cagagccgct gatcagcgaa atggaagctg
    34141 ttattaacaa aacccgcgcg gaaattttac atggcatctc cgcagaggaa ctggaacaac
    34201 tgattacgct catcgcaaaa cttgagcata atatcattga gttacaggcc aaagggtgaa
    34261 atgaaggggg cgtgtggtca gtaacctgac cacacgcaaa cttatttagc gcggagaaac
    34321 ggtcacctga ctgccattgc tggccagtac gacacgttgg cccggagaga aacgagtgtt
    34381 gccttgtttc tgtaccacca tgatggtatt accatcgtct ttacgaattt ccagctcgac
    34441 accttgcgtt ttgttcattg cactctgtac gccctgacca gctacgccac ctgcaacagc
    34501 gcctgctgca gtagccagag aacgcccggt tccgccacca acagtatttc ccaggaaacc
    34561 accaagaaca gcaccgccaa ttgcaccgat aacgttggaa tcatcaccgc cctgaatctg
    34621 taccggacgt acgttaacga tggttccata gctgacattc tgtacttgtt tcgcttcaga
    34681 agcggtataa acatcccctg acagggtgtc gttattaaca caaccgacaa gagacagacc
    34741 taccattgaa acaaccaata cgcgtttaat cattgaaaaa tctcctgttc accatgaaac
    34801 gccacgcaag catccctcat ggtcaaagta tatggcatat gtgaatccat ggttacatgt
    34861 tctaccaaat atgagcaaat cataatgaaa gtaggatcaa ccaattataa acaaaacatt
    34921 catgatgatt attgatttga agataatgtt ttactaccaa cggtaaaaat cggtcattcc
    34981 gcatggtatt cacgaaaagt ttatgtttga gtgtgcgcaa acgtcagatt gaaaaaggat
    35041 gaactatgaa atcgggccgc tttattggcg ttatgtcagg caccagcctt gatggtgttg
    35101 atgttgtgtt ggcgacaatt gatgaacacc gggtcgcaca gctggcaagt ttgagctggc
    35161 cgatcccggt gtctctgaaa caggctgtac tggatatttg ccagggccag cagcttacac
    35221 tttcgcagtt tggacagctt gatactcaac tcgggcgact ttttgctgat gcggtcaagg
    35281 ccttgcttaa ggaacaaaac ctgcaggcga gagatatagt tgcgatcggc tgtcacggtc
    35341 aaaccgtctg gcatgaaccg acgggcgtgg caccacacac tttacagatt ggcgataaca
    35401 atcaaattgt ggcacgcacc ggaattacgg ttgtcggtga ttttcgccgt cgcgatattg
    35461 ccttgggagg acaaggcgca ccgctggtac ctgcgttcca tcatgccttg ctggctcacc
    35521 caaccgagcg acgaatggtg ctcaatattg gcggcatcgc caatctgtca ctgctcattc
    35581 ctgggcagcc ggttggcggc tacgataccg gtcctggtaa catgctgatg gatgcctgga
    35641 tctggcgtca ggccggtaaa ccttacgata aagatgccga gtgggcacgg gcgggtaaag
    35701 ttattctccc actgctgcaa aatatgctca gcgacccata tttctcgcaa cctgcaccga
    35761 aaagcaccgg acgcgaatac tttaactacg gctggctgga gcgccattta cgccattttc
    35821 cgggtgttga tccccgtgat gttcaggcca cactggcaga actcaccgcc gtgaccattt
    35881 ctgaacaagt tttgttgagc ggtggctgcg aacgattgat ggtatgtggt ggaggtagtc
    35941 gtaatccgct actcatggcg cgtctggcgg cattactgcc aggcacagaa gtcaccacca
    36001 ccgatgccgt tggcattagt ggcgatgaca tggaagcatt ggctttcgct tggcttgcct
    36061 ggcggacgct ggcgggatta ccaggaaatc tgccttccgt cactggcgca agccaggaga
    36121 cggttctggg ggctattttc cccgccaact cgtgatatca gaattaactg aattttttct
    36181 tatcgtgtct gccgttacac ttggaagatt aaggaggata tttgccctcc ggaccaggaa
    36241 agtcttcggg atatgactat gaaaaaactg ttaattatca ttttgccggt ccttctctcc
    36301 ggctgtagcg cctttaatca actggttgag cgaatgcaaa ccgatacgct ggaataccag
    36361 tgtgatgaaa aaccgttgac ggtcaaactg aataatccgc gccaggaagt cagttttgtt
    36421 tacgataatc aactactgca tctcaaacag ggcctttcag cctctggcgc gcgttactcc
    36481 gacgggatct atgttttctg gtcgaaaggt gaggaagcga ctgtctataa acgcgaccgc
    36541 atcgtcttga ataactgtca gttacaaaat ccacagcgtt gagatttttc caggggcggc
    36601 gcacaatagc gtcacccaca gacaatccgt aaagaaaacc atgtctgata acgacgaatt
    36661 gcagcaaatc gcgcatctgc gccgtgaata caccaaaggc gggttacgcc gccgcgatct
    36721 tcccgccgat ccattaaccc tttttgaacg ttggctctct caggcttgtg aagccaaact
    36781 ggcggaccct accgcgatgg tggtcgctac cgtggatgaa catggtcagc cttatcagcg
    36841 catcgtttta ctcaaacatt acgacgaaaa aggtatggtg ttttacacca acctcggtag
    36901 ccgtaaagca catcaaattg aaaataatcc gcgcgttagc ctgctgttcc cgtggcatac
    36961 ccttgaacgc caggtgatgg tgatcggtaa agcagaacga ctttcgactc tcgaagtgat
    37021 gaaatatttt catagccgcc cgcgtgatag ccagattggt tcatgggttt cgaagcagtc
    37081 cagtcgcatt tctgcccgcg gtatccttga aagtaaattc ctggagctga agcagaagtt
    37141 tcaacaaggc gaagtgccat tgccgagctt ttggggcggt tttcgcgtca gccttgaaca
    37201 gattgagttc tggcagggcg gtgagcatcg cctgcatgac cgctttttgt accagcgtga
    37261 aaatgatgcg tggaagattg atcgtcttgc accctgaaaa gatgcaaaaa tcttgcttta
    37321 atcgctggta ctcctgattc tggcacttta ttctatgtct ctttcgcatc tggcgaaaag
    37381 tcgtgtaccg gcaaaggtgc agtcgttata tacatggaga ttttgatggc aagcagtaac
    37441 ttgattaaac aattgcaaga gcgggggctg gtagcccagg tgacggacga ggaagcgtta
    37501 gcagagcgac tggcgcaagg cccgatcgcg ctctattgcg gcttcgatcc taccgctgac
    37561 agcttgcatt tggggcatct tgttccattg ttatgcctga aacgcttcca gcaggcgggc
    37621 cacaagccgg ttgcgctggt aggcggcgcg acgggtctga ttggcgaccc gagcttcaaa
    37681 gctgccgagc gtaagctgaa caccgaagaa actgttcagg agtgggtgga caaaatccgc
    37741 aagcaggttg ccccgttcct cgatttcgac tgtggagaaa actctgctat cgcggcgaac
    37801 aactatgact ggttcggcaa tatgaatgtg ctgaccttcc tgcgcgatat tggcaaacac
    37861 ttctccgtta accagatgat caacaaagaa gcggttaagc agcgtctcaa ccgtgaagat
    37921 caggggattt cgttcactga gttttcctac aacctgttgc agggttatga cttcgcctgt
    37981 ctgaacaaac agtacggtgt ggtgctgcaa attggtggtt ctgatcagtg gggtaacatc
    38041 acttctggta tcgacctgac ccgtcgtctg catcagaatc aggtgtttgg cctgaccgtt
    38101 ccgctgatca ccaaagcaga tggcaccaaa tttggtaaaa ctgaaggcgg cgcagtctgg
    38161 ttggatccga agaaaaccag cccgtacaaa ttctaccagt tctggatcaa cactgcggat
    38221 gccgacgttt accgcttcct gaagttcttc acctttatga gcattgaaga gatcaacgcc
    38281 ctggaagaag aagataaaaa cagcggtaaa gcaccgcgcg cccagtatgt actggcggag
    38341 caggtgactc gtctggttca cggtgaagaa ggtttacagg cggcaaaacg tattaccgaa
    38401 tgcctgttca gcggttcttt gagtgcgctg agtgaagcgg acttcgaaca gctggcgcag
    38461 gacggcgtac cgatggttga gatggaaaag ggcgcagacc tgatgcaggc gctggtcgat
    38521 tctgaactgc aaccttcccg tggtcaggcg cgtaaaacta tcgcctccaa tgccatcacc
    38581 attaacggtg aaaaacagtc cgatcctgaa tacttcttta aagaagaaga tcgtctgttt
    38641 ggtcgtttta ccttactgcg tcgcggcaaa aagaattact gtctgatttg ctggaaataa
    38701 tgcattaagt ggaaaggggg agtgagaaat cactccccct ggtttttata cagggaacat
    38761 gatgaaaaat attctcgcta tccagtctca cgttgtttat ggtcatgcgg gtaacagtgc
    38821 ggcagagttt ccgatgcgcc gcctgggcgc gaacgtctgg ccgctgaaca ccgttcaatt
    38881 ttctaatcac acccaatacg gcaaatggac tggctgcgtg atgccgccca gccatttaac
    38941 cgaaattgtg caaggcattg ccgccattga taaattacac acctgtgatg ccgtattaag
    39001 tggctatctg ggatcggcgg agcagggtga acatatcctc ggtatcgtcc gtcaggtgaa
    39061 agcagcgaat ccgcaggcga aatatttttg cgatccggta atgggtcatc cagaaaaagg
    39121 ctgtatcgtt gcaccgggtg tcgcagagtt tcatgtgcgg cacggtttgc ctgccagcga
    39181 tatcattgcg ccgaatcttg ttgagctgga aatactctgt gagcatccgg taaataacgt
    39241 cgaagaagcg gttctggcag cgcgcgaact cattgcgcaa gggccacaaa ttgtattggt
    39301 taaacacctg gcgcgagctg gctacagccg tgaccgtttt gaaatgttgc tggtcaccgc
    39361 cgatgaagcc tggcatatca gccgtccact ggtggatttt ggtatgcgcc agccggtagg
    39421 tgttggtgat gtgacgagcg gtttactgct ggtgaaactg cttcaggggg caacgctgca
    39481 ggaggccctg gaacatgtga ccgctgcagt ctacgaaatc atggtgacca ccaaagcaat
    39541 gcaggaatat gagttgcaag tggttgctgc tcaggatcgt attgccaaac cagaacatta
    39601 cttcagcgca acaaagctct gaaatatctc atttcggccc acaacgaact gtgggccgac
    39661 ggctattact ttaagccttc cgctgacagc gcgtcttgta cttccggacg ttcagccata
    39721 cgttgcataa atgccgcaat gtgctctaac ccttccagat tcagtttcac cgcgtatgcc
    39781 cagcgcagta ccgtaaacag ataggcatca gcaattgtaa atctttgccc gcagatccag
    39841 tgctcatcct tcagtgcctc gttcacatat tgcagtttct tctccagctg cgcgcgaact
    39901 gtcggtttgt actcttccgg tgtatcaggg cgaaacagag gtgtgaaacc tttatgcagc
    39961 tcggtggcga tgtaattcag ccattcgatg gttttatagc gggaaatact gtttaccggt
    40021 gccagcaact ggcggtcggg gacgctgtcg gcaagatact gcataatcgc tacaccttcc
    40081 gtcagcaaag taccgtcatc cagcagcaat gcaggtacct gtcccttagg gttaacggca
    40141 aagtaatcgt caccgttttc gagacgtttt ttcattaaat ccacactgac gagcgtaaaa
    40201 tcctttccgc tctcacgcag ggtgatatgg gaagcgagag agcaggcgcc cggtttgtag
    40261 aacaatttca tcggtaactc cttttgtctg tggtttaaag tatggtagtg cgttgctgaa
    40321 caaaaaaaaa gccgctaacg aaaagttagc ggcttgtaaa agagtttccc tgaattttac
    40381 gctacggctg ctttcgccgc tttgtctgca gcgtcatcct gcgtcatgcg gtgcagtttc
    40441 ggcgcggtca gcagcatcag tactgcaata acggcagtag cgacaccaat ctgcaagaat
    40501 acgcgaccat agacttccag tgacatcagc ggatcggtaa cgttatccgg cacagccatc
    40561 atacccgcaa cataaccacc aatcaggttt gcaccggcag tggtcaggaa ccagctaccc
    40621 ataatgaagc ccatcagacg ctgcggaacg agttgtgcaa ccattgccag acccagacca
    40681 gagatcatca gttccccgat actctgcagg ccatagctcg cgaccagcca gcttacagac
    40741 acgatacctg cgtcagacgc gaatttcgca cccagcggca gaatcaggaa cgcaccagaa
    40801 cacatcacca taccgattgc aaacttggtt ggcatcggca gggtatcgcc catcttgtta
    40861 tagatagcgg ccagaatcgg gctaccaatg atgatccaga acgggttcag tgcctgatac
    40921 tgctcaggtt ctacggccag gcccagaatg gtatgttcaa cgttacgaat cgcaaagaag
    40981 ttcagtgacg ttggcatctg gctgtacagc acgaagaaga taatggcttc gagcatcagg
    41041 atgaaggcaa cgatcatttt acgacgcgca gcacctttca tcgcgaaggc ttctttaccg
    41101 aagataacca cgataccgaa ggcaacaacg cccagcgcca tacgcgcaac ttcctgattg
    41161 tgcagcagcc aggtggcgat agcgatcagt gccacaacac caataatggt cagcagcagg
    41221 ttacggtagt tgataggctc gaagtctggt tttgaaccgt actgtttaac ccagcgttgg
    41281 cagaaggcga agttaacgat agtgatcagc aggcctacaa cgctcaacgc aaacgcaaca
    41341 ctccagccgt atttcgcggc cagccacggg gtggcgatca tagagaagaa agagccgatg
    41401 ttgacggaca tgtagtacat ggtgaatgca ccgtccagac gcgggtcgtt tttctcatag
    41461 catgtagaaa gcagagaaga cgggttagct ttaaacaggc cgttaccgac cgcaatagcc
    41521 gccataccca tataaacgat accggcgtcg tgaccagacc aggcaaccag agcataacca
    41581 atcgccagca caatagcgcc gagcataatt acgcgtttag tacccagtac cttgtcacct
    41641 aaccagccgc cgatagcgac cagaccataa accagggcac taaaggaaga gaaaagggtg
    41701 attgaatccg cttcagacat acccagttgt ttaaccaggt aaacagccat aattccttgt
    41761 aggccgtaat aaccaaaacg ttcccataac tcaatcgaga agatgagata gaacgccttc
    41821 ggttgtttga aagcgttcaa actgacgctt tcagttggtt tttggtttgc agtggacact
    41881 tttacctctt tttttacatc ccatattaac gggggtgctt tctcgcgtta tgtctaaaaa
    41941 acatacgcgc aattgttata gtgggagggg aaacggcagg taatgttcac tatcttgcac
    42001 tttcagacaa gtcctttgta ataatctgtt acatataact gaggcgggga attcaaccat
    42061 tccgggaaat gttatccagc gttaaatttt tcaaaaaacg taacggcaga ttttttcact
    42121 gcattgagag gggggtgatg gcttgacggc gatatgttct gctatttagc gtttttccta
    42181 gtgagatagt ccagtttctt aaaaatagcc agtgtaatgt tttgtaggtc aatatttgtt
    42241 gcgtgtgtaa atttaaatga ttttaacctg attcttgcaa tgtttttaca ttgccacagc
    42301 atcgtgatct tgatcactaa tgaatagaaa atttcgctat taaaaaaaca attaacctgc
    42361 atttttggtt atccgcaaac ggattacctg atgataacgc taagcaatgg catcattgac
    42421 gttaaaaaga tgttttaaaa gaggataaag aaaggttatc aatggggtaa tcggtgttac
    42481 cccttttctt cagatgtcaa ctttctcttt gtattcacaa agatcttcaa taatacaaga
    42541 gccacagcgg ggcttgcggg caatgcaggt ataacgcccg tgcaggatca accaatggtg
    42601 gcagtcgact ttaaactctg ccggaaccac tttcagtagc ttttcttcta cctgttcgac
    42661 gtttttcccc ggcgcaaatt gagtacgatt acaaacgcgg aaaatgtgcg tgtcgacagc
    42721 aatagtcggc cagccgaatg cagtgtttaa tacgacgttg gctgttttac gacctacgcc
    42781 gggcagggct tcaagcgcag cacgatcttc cggaacctcg ctattatgct gctccagtaa
    42841 gatacggcag gttttgatga tattttctgc tttgctgtta taaagcccaa tcgttttgat
    42901 ataggttttc accccttcaa cgcccagttc aagcatcgct gcaggcgtat tcgccaccgg
    42961 gtagagtttc gccgtcgcct tattaacact gacatcggtt gcctgagcgg aaagcagtac
    43021 ggcaatcagc aattcaaaag gcgaactgaa attaagctca gtggtgggat gaggattgtt
    43081 ctcacgcagg cgagtgagga tctccaggcg ttttgctttg ttcatcagac attccctgtt
    43141 tcaccgtttg gcaatgcacg ttcggcagct gcttcagtac ggcgcttttt cattctttca
    43201 tcaatcaggt attttcctgc cagcatcagt cccaggccaa taaatgcacc tggtggcagc
    43261 atcgccagca ggaaagggga gtcggtgtgg aaaatctcca cgcgtaatac ttttgcccag
    43321 ctacctaaca gcgcatctgc accgtcaaac aatgtgccat tgccgataat ttcgcgtagt
    43381 gaacccagca cgaacatggc gcaggttgcg cccataccaa ttgaaaagcc gtccagtgcc
    43441 gaaagcgccg gacctttttt ggcagcgaag gcttcagcgc ggcccacaac gatacagtta
    43501 gtgacaatca gcggaataaa aatccctaat gattgataca ggccaaaggc gtaggcgttg
    43561 atcagcatct gtacagcgct gaccaccgag gcgatgatca tcacgtaaat gggaatgcgg
    43621 atctcggctg gcgtccagtg acgcagcgtc gaaatggtca ggttggtcag cgtcagtacc
    43681 agcgtagtcg caagtcctaa acccagagcg ttagtggcag tagacgtgac agccaacaga
    43741 ggacaaaggc cgagcaactg gaccagcgca gagttgtttt tccacaaccc ctgaacaata
    43801 acgtctttaa tttcgctcac gctttattct ccacaggcag gaagttgaga aagttgttcc
    43861 ggtaacgtct gagcgtacaa tccggcgcgt tttaccgcat taaccaccgc gcggggagta
    43921 atcgtcgcgc cggtgaactg gtcgaaatta ccaccatctt tcttcaccgc ccagtgcgca
    43981 tcatccgcac cactgatttt tttacccgca aaatgggtga tccagtcaga aaggcgcagt
    44041 tcgattttat cgccaagccc tggcgtttcg tggtgctctg tcacgcgcgt gccaagtacc
    44101 gtgccgttaa aatcggctcc caccagcagc tgaatcgcac cggaatagcc atctggcgcg
    44161 gttgcttcca gaacggcggc taccggtttg tcatcctgtt tggcgatgta aacccgatgc
    44221 tcacctttac ctaactctgg cgcagttacc agatagcaac tctgtgccag cgcattgtta
    44281 tagcgttcgg ctggcagcac ctgatcaaat aaagcctttt gttgcagact ggcctgttca
    44341 gcaatcgtcg ttttggtcat ctggttgatg gccgcagtta accctgttga acccgctgca
    44401 aatagcgcca acgtaatgcc gtgttttcgg atagttttca gcatggttta ccctttgcga
    44461 tggccgtaga cgcgcggacg cgtgtagtaa tcgatcagag gaaccgtgat gttcgccagc
    44521 aggacggcaa aagccacgcc gtcaggatag ccgccgaaac tgcggatcaa ccagactaat
    44581 aagcccgcaa gcgcgccgaa aataagacga ccacgattgg tcgtagaagc ggtaaccggg
    44641 tcagtcaaaa taaagaatgc gccgagcatg gtcgctccag acagcagatg aatttgcggt
    44701 gctgccagtg tttctggtga gaacaaccag cccaacgttg cgcataacgc cagcgttact
    44761 aagaagctga ggggaatatg ccagcgaatc gctttctgcc atagcaacca tacgccgcca
    44821 gccagccagg cgagatttac ccattgccaa ccagcgcccg ccagaatacc gctgtagatc
    44881 ggatattgca taatctgttc aaccgaatga ccggcacgga cagaggtttt aaatgtatcc
    44941 agcggtgtcg cctgactaat gccatcaata cctaagcgta gtgtgttcat atcaccacca
    45001 ctggcggtat gcccgctgaa aataacctgt atggcgtcga taaaaccagg gatgttgacc
    45061 gcaatttcat gtggcggtaa ccagctggtc atctgcaccg ggaaggagat cagtaagacc
    45121 acataaccaa tcattgccgg attaaacggg ttttgcccca gaccgccata caactgttta
    45181 gcgatgataa ccgcaaacac cgtacccagc acgaccatcc accatggcgc gaggggggga
    45241 atacttaccg ccagcaataa gcctgtcagc aatgctgagt tatctttcaa cgtggcggct
    45301 accgactgct tgcgtagttt gagtacgaga gcttcggcta acagagcact aaccgatgcc
    45361 aacaggatct gaacgagagt accccaacca aaaaaccaca gttgcgctgc gattcctggc
    45421 acggctgcga gcaacaccaa cagcataatg cgcgatgtct ggcgctggtt atgggtataa
    45481 ggggagctag ctattctgaa taccatttag tcctcgttta caaccttttg ctggacggct
    45541 tttttggcct gaacgcgggc aatagccgcg gcaactgccg ctttgcgcgg atcaacctgt
    45601 tcttctggtt ctgcattagc ctgttgctgt tccagcttgc gtgctttggc gcgtgcaata
    45661 gccgctgcga cggcagcttt gcgcggatca acctgttctt ctggctccgc attagcctgt
    45721 tgctgttcca gtttgcgcgc tttggcacgg gtaatagcgg cttcgacggc ggctttgcgc
    45781 ggatcgaccg gttcttctgg ttccgcatta gcctgttgct gttccagctt gcgtgctttg
    45841 gcacgggcaa tagcggcttc gacggcggct ttgcgcggat cgaccggttc ttctggttcc
    45901 gcattagcct gttgctgttc cagcttgcgc gctttggcgc gggcgatagc ggcttcgacg
    45961 gcggctttgc gcggatcgac ctgttgttct ggttccgcat tagcctgttg ctgttccagc
    46021 ttgcgtgctt tggcacgggc aatagcggct tcgacggcag ctttgcgcgg atcgacctgt
    46081 tgttctggtt ccgcatttgc ctgttgctgt tccagcttgc gcgctttggc gcgggcgata
    46141 gctgcttcaa cggcagtttt acgtggatca gtaacggttg ctgcgtcgtt agtttgctgc
    46201 agttctgcct gtttcgctct ggcttgcgct ttacgggctt cccgtgctgc aataattgca
    46261 ctgttatccg ggcgttcgcc cgctttaatc acaataggct gtgtagcctg ggcctgtttc
    46321 tctttcaccc gcgccagagc ggcagcaatc gcatctttat ctttggctgc aggttgaacg
    46381 gctgcgctct tatgtcgttc aaggcgagcc gctttttcgc gctccagacg agcctggcgc
    46441 gcttcgaaac gcgctttggc ttctgcggcg cgcttttctt cctgacgaat agccgcaatt
    46501 tcagcttttt cctgacggaa atattgcacc aggggaatat tgctcgggca aacccacgcg
    46561 caagccccac attcaatgca gtcggcaatg ttatgcgtgg tagctttatc gtgttgctga
    46621 cctttgctga accagtacaa ctgttgcggc aaaagatccg cagggcaggc gtcagcacag
    46681 gcgctacacc ggatgcagct ttgttcttcc tgtggttcgc caagctcatt ggcagaggga
    46741 gccaacagac agttggtaat ctttacgacc gggacatcca gccatggcaa ggtaaagccc
    46801 attagcgggc cgcccataat caccatttga tcggcagagg ggcagaatcc ggcgtcattc
    46861 aataaatgac gcactggcgt ccccagccgt gcccagacgt tgcccgggcg agcgattgct
    46921 tcgccagtca gggttacgac acgctcggta atcggctcgc catcaataac ggcacgtttc
    46981 actgcataag cagtgccgac gttttgcatt aatacgccga tatcggatga acgcccgcca
    47041 tgtggaacct gcttcccggt cagaatgtag gttaattgtt tagcaccgcc agaaggatat
    47101 ttggttggaa tcacccgcat agaaatatcg tgagagtccg ccagcaccgc gcgcagcatg
    47161 gaaatcgcct gcggtttgtt atcttcaatg ccgataagaa tttcgcgtgg ctgcagaata
    47221 tgcgcaagaa tgcgaatacc ctctacgacc tgagccgcgc aatcctgcat caaacggtca
    47281 tcggcggtaa tgtatggctc gcactcagca gcgttgataa tcaacgtctc aatcttatct
    47341 ccgccgccct gcaatttaac gcctgtcggg aatcctgcac cacccagccc ggcaacacca
    47401 aactgatgaa tgcgctcgat taactcttcg cgacggcgag agcgataatc ggcccagccg
    47461 tctcgtggga tccagcagtc ttcaccatcg gcatcaataa tcacgcttaa ttcagctaaa
    47521 gctgaaggat gagccgtaga gtggggcgca atagccgtaa cggtacccga ggtgggcgcg
    47581 tgaacaggca gcattttgcc gcgaccacgg gtaagcggct ggccgcgcaa tactttatcg
    47641 ccgacgctaa cgcacaactc accttcagcg ccaatatgct gtttcagtgg aatgacaaaa
    47701 cgctgcgcca gtggtacctg gcgcaggggt gtaccgttgg actgggtttt catctccggt
    47761 ggatggatgc cgccgttgaa atcccagatt ttattttttc tgaatgcaga gaataactta
    47821 agcatggtgt tccacgggaa tgatacgcac gggaatggtg ttcagatccc atttccagga
    47881 gtcaggtgtt tctgcgaccg gttgcaacga gatgcagtgc gtcgggcacg gatcaacaca
    47941 taaattgcag cccgtacaga gatcactcat taccgtatgc atggcacggg tagcgccaac
    48001 gatggcgtct accggacacg cctgaataca tttagtgcag ccaatacagt tattttcatc
    48061 aataaccgcc accatccgcg caggcgttag ctcttgcgct tcgccatcca gcggctgcgg
    48121 ctcgacatta agcaactcgg caatttttag catcacagct tcgccaccag gggcgcaacg
    48181 gttgattttt tcaccgttac agctgatggc ttccgcgtag gggcgacagc cgggataacc
    48241 gcactgacca cactggctct gcggtaagat ttcgtcaatt ttctcaacga ccggatcgtc
    48301 ttccaccgca aaacggcggg aggcataacc cagaatggcg ccaaacgcca ggcccagcag
    48361 gctcacggcg gcaacggcaa tccagatagc attcattaca acttcaccaa accactaaag
    48421 cccataaagg ccagagacat aagacctgcg gtaattaacg caatggcatt accgcgaaaa
    48481 ggtgccggga catcagccac agcaaggcgt tcgcggatgg ctgcgaagag aaccatcacc
    48541 agcgaaaaac cgacagcggc ggaaaaaccg tacagcgccg actgcaagaa attgtgcccg
    48601 agattgatat tcagcaacgc cacgccgagc acggcacagt tggtggtgat aagcggcaaa
    48661 aaaatcccca gcaaacggta aagcaccggg ctggttttgc gcaccaccat ctcggtgaac
    48721 tgcacgacca cagcaatcac cagaataaat gccagggtgc gcaggtaaat cagattaagt
    48781 gggatcaaaa tccacgtatc gataagccag gcgcaaatag acgccagcgt catcacaaac
    48841 gttgttgcca gccccatgcc catcgcggtt tccagctttt tggaaacccc cataaacgga
    48901 cagagaccga gaaacttgac cagtacaaag ttattgacca gtacagttcc gacaaacagt
    48961 aacaggtagt cagtcatttt tcggcctgaa ataaaaaagc cgcctattat ccgttaatcc
    49021 agagcaggcg acaacaggtt aactgtaagg ttattacgga ttcacaaacg tcgctttcac
    49081 ccgcgacgag cgtttgaaat aggggacgat caatgcggtt gccaacaaag taaacagcaa
    49141 ctggcgaacg gctatgccat cttccacggg tgaaaaagcg aacgctttta ccgccagcaa
    49201 tacggaaata agcagccaga taatatagtg tttaggaaca caacgccgac gtttaaagaa
    49261 agcgatggtc aaccacagtg tgtaatacca catggcgata gcggtaataa aagaaacgcc
    49321 ccataagatt tgtgtcgtca gtgcttgtgc gccgagcgtt tgaaatgttt gcggagaaga
    49381 taacgcagcg gtatacagca acaacgccag cgtcgtactt aacagagcga ctaaaagcca
    49441 ggccaacggg ccaagcaacc agcctccaat acgttgtggc gttgtggtgg tcataggttc
    49501 tccagaatag cgacgcaaac aggcaaatct aattttaggg gcgttattat aaggctttta
    49561 taaccgatcg ctactttctt tattgaacat agtgccagac ggacttcggt acctggccga
    49621 ggtcaaacaa acgcccgcca gaaaccagct ctgcgcgacg gtgatcggca gcacgataca
    49681 tattgatcag ttcctgatcg tccgtcaggg tgtaattaag atggtcgtag agtttttcca
    49741 gactttcgag tgaactgatt ttgcgaaatt ttaataagta gtcctgaaca gtcataaaag
    49801 gtccataaaa taattaatac caaaggggta ggtattatta gtgatgataa aatttcacca
    49861 aaagtgaacg cttgtgttat tacaataata agaatgcgct gatggcaaat attcagggat
    49921 gaaaaactgc tatgaaaaga gtaacgcgct ttactatccc tgacaatcac caacaacacc
    49981 gaacaagata ataaattcct ggtttaatat ccgacaagtg aaaacatgca ccctgacggg
    50041 cagcatgtcg ctctacaagc gcagggctta cttgtgttgt accgtagtac tctgttcagg
    50101 tggctgatag ttgtcaatgt gactcgccac gccaagaaga atgactgaaa cgacaagaac
    50161 gatccaacct gttaattcaa taagacgatt cattacaact cacatctctg ttgattgatc
    50221 cattaacttc agggggtaaa tgttacttag caataatagc tccagcaaga tttttactga
    50281 ggtttttgcg atatttagct tttgtcgttg gaaaaatcgc tatttttttg acttaagtta
    50341 aacaacatcc cttattgctg gcaggttatt aaactgttga gcgagggtaa aggatagtgt
    50401 caaatagcca tcatacttca atgagaggca atgacatgag cgacaacatc cgtgttgggt
    50461 tgattgggta tggttatgcg agcaaaacct tccatgcgcc cctgattgcg ggcacacccg
    50521 ggctggaact ggcggtaatc tccagcagtg atgaaacaaa agtaaaagcc gactggccaa
    50581 cggttacggt tgtctctgag ccgaagcatc tgtttaacga tcccaacata gacctgattg
    50641 tcattcctac acccaacgat acccatttcc cgttagccaa agcagcgctt gaggcgggta
    50701 aacatgtggt cgttgataaa ccctttaccg tgacactgtc acaagcgcga gagctggatg
    50761 cgctggcaaa aagcctgggg cgtgttctgt ctgtattcca taaccgtcgt tgggatagcg
    50821 atttcttgac gctaaaaggt ttgctcgcgg aaggcgtgct gggtgaagtt gcttactttg
    50881 agtctcattt tgaccgcttc cgtccgcagg tgcgcgatcg ttggcgtgaa cagggcggcc
    50941 caggcagcgg tatctggtac gatttagcac cacatcttct tgatcaggcc attacgctat
    51001 ttggtttacc ggtcagcatg acggtagatt tggcacagtt acggcccgga gcgcagtcga
    51061 ccgattattt ccacgccatc ttgtcctatc cacagcggcg agtcatttta cacggtacca
    51121 tgctggcagc cgctgagtca gcacggtata tcgtgcatgg atcccgaggc agttatgtga
    51181 aatatggcct cgatccacag gaagaacgtc tgaaaaatgg cgagcgtctg ccgcaggaag
    51241 actggggcta cgatatgcgt gatggcgtac ttacccgcgt ggaaggtgag gaacgtgtcg
    51301 aagaaacgct gttgacggtg cctgggaatt atccggctta ctatgcggcg attcgtgatg
    51361 cgttaaatgg cgatggtgaa aatccggttc cggcaagcca ggcaatccag gtaatggagt
    51421 tgattgagct gggcatcgaa tccgccaaac atcgcgcgac tttgtgcctt gcatgaaaaa
    51481 tggatggtgc aatttgcacc atccattttt gttacttcgc tgcgactttt tctcgcagtg
    51541 cgcgtttttc ctctgcgttg aggaaagcca tttccagacc attaatctgt gcctggcgga
    51601 tttgctcgcg ggataaccca gcagctggcg cggcaacggt atattcgtga atgatatcca
    51661 ctccctgtac gccgggatcg tcagtgttaa tgctggcacg aatgccatgc tcaaggaacg
    51721 ttttcagcgg atgtgcagcc agctctgcca cggtgctggt ctgaatattg gaggtcagac
    51781 aggattcaat accaatttgt tgctcggcga gaaaatccat cagcgcccga tcttcaatgg
    51841 cttttacgcc atgtccaatg cgctctgccc ccagttcacg aatcgcctgc cagatgcttt
    51901 ccggcccggc agcttcgcct gcatggacgg taatatgcca gcccgcatca cgcgcgcggt
    51961 tgaagtgaga aagaaacaga cttcccggga aaccaagttc atcaccggct aaatcaagtg
    52021 cggtaatctg gtcacggtgg gctaaaaagg cctccagctc ttgctgacag gcggcttcgc
    52081 cgaaggttcg gctcataatg ccgataagct tcgcctgcac accaaaggtg cggcaacctt
    52141 cacgtacgcc atcgatcacc gcttcaacaa cacctgcaac aggcagctga tgtgccattg
    52201 ccatgtagcc tggtgaaaaa cgcagctcga catagtgcag gccgttacgg gctgcatctt
    52261 caatgttttc aaatgccacg cggcgacaag catcaagaga ggcgagaact ttaacgcccc
    52321 agtcaagttt ggtcagaaag ctcaccagat cgggttcgtt ggcaatgacc tgaacgtggg
    52381 gaatcagtgt ttccagggat tgtgcaggaa gcgagatatt atactggcgg ccaagttcaa
    52441 gaatggtctg gggacgaatg ttgccatcaa ggtggcgatg gatatcagtt aatggcaggg
    52501 tggtatcaat catggtcgca ctctttttct ggatataaaa gtgcgcacca ttataaaaac
    52561 aaagcagggt aaaaatccac gattgcgcaa cgatttttta cgttgcgcaa ttggggttag
    52621 cgaacagcgc ggatggcgtt aattaatcca gccacacctt tttccagttt cgaacgtggg
    52681 cagccggcat tgagtcggac aaaaccacga ccttcttcac cgtaggtata ccccggcatg
    52741 atcgcgactt tttcttgttc gataagtgct ttttgcaacg cgttgtcgtc aatattcaac
    52801 ggacgtaaat caagccaggc cagataagtg gattgcggga tctgccagtt gagttcagga
    52861 aacgcggcgt tcattttatc tgcgatatac gtcaggttat ctttcagata gacgcgtaag
    52921 gcatccagcc acggtgcacc ttgctgatag gcggcgatat gggcagttaa cgccagtacc
    52981 gaaggggaag aaagcccatc acgtcctttc agtgccgata aataggcatc acggctgctg
    53041 ctattttcta taatcccgta agcaccggtc agcgcgggaa tattgaaact tttcgatccc
    53101 gacgtcagca acgcccagtc tccccgcgcc acattactcc agggaatatg cggctgctcg
    53161 ccccaaacca tatccatatg gatttcatcg gaaataaccc gcacaccatg acgctcgcac
    53221 aggtcagcca tgatctccag ctcatcgcac gtccacactt ttccggtagg attctgtggg
    53281 ctacacagga gcataatttt acattctggt ttcgccaaca cggcttccag cttgcccata
    53341 tcgcaaaacc agccgtcagc ctgcttctct aaagcaacgg gcattactgt gcgctgatta
    53401 ccttcaatgg ccttgtaaaa tgcgtcatag gcgggtgtgt ggatcaccac gccttcacct
    53461 gtttcagacc actgacgaat cagttctgag accatataga taacagaagg gccatacacc
    53521 accgactgag gatcgatggc ggtgtaatgc tgggtggaaa accagtgggc aatagccgcg
    53581 agaaactcat cgttcttcca gcggctgtag ccaaacacgc cgtgcatcag gcgctgattc
    53641 agcgcctcga taatgcaggg ggcagtggca aaatccatgt ctgaaatcgt gaaaggtaac
    53701 aggtcagcag tgccgaaacg gtcagcgaca taatcccact gcgtacacca tgtgccatga
    53761 cgatccacga cctttgaaaa atcgaacata tcttatcctt atgcctggac agtatgcatc
    53821 agaccggcca tttcatcttt tactgactga acttgtggcc cgataacaac ctgcaggtta
    53881 tgttgattaa gttgtactac gccaattgcc cgattgtcct tcagtgcctg cacattaaca
    53941 agcgacatat ctttcacaga caaacgcaga cgggtaatgc agttatcgag gctaacaata
    54001 ttgtcggcac cgcctaaggc ttcgaggatt gccggaacgt tgtaacctga tttacccggc
    54061 gcaccggcaa cggctttttc gattgaactg gccacttcgc tatcgcgccc cggggttttc
    54121 agattgaagc gggtgatagc gaaacggaag atgacgtagt aaacgacaaa ccagattgcc
    54181 accaccactg gcaccatgta ccacttggtt gacagaccat gcaaaatacc gaacaccacg
    54241 aagtcgatga tattgccgtc ggtattaccg atggtgacgc cgagcacaga catgacggtg
    54301 aagccgaggc cggttaacag cgcgtggatg acatacagaa ctggcgctac gaacaggaac
    54361 aggaattcca gcggttcggt cgtgccgcca acgacacagg cgatcaggcc agaaatcagc
    54421 agccctttaa ttttatggcg attttccggg cgcgcgcagt gatacatcgc taacgctgca
    54481 cctggcagac cgccgagaaa cgcaggcatt ttgccttgcg aaaggaaacg cgtggcgctt
    54541 tcagaaaaac cgtgagtggt cgggcaactc aattgcgcct ggaagatggt caatgcgccg
    54601 ctgacggttt gaccgcagac ttcctgcgtg ccacctgcgt cggtaaagcg aattaatgcc
    54661 accagaatgt gatgcagacc aaacggcaac agcagacgtt caccggtacc aaacagcatc
    54721 ggtccgaaat cacccgcgct attgatcata tggcctaagc cgctaatacc catggcgaaa
    54781 atcggccaga ctaatggaat caccaggccg acaaggccca tcaccagcga ggagatgatt
    54841 ggtacgaagc gcgtaccgcc gaagaatgcc agcgcatccg gcaggcggat attatggaat
    54901 cgctcatgca gcatccagac gataataccg gcgatcaccg caccgaggat cccggtgtcg
    54961 atcgactgga tcccaagaat gctctggatg ttattggctt tcagaacggc ggcatccgtg
    55021 gttggcagaa tgcctttatt ggtcaaccag aagtttaccg cgaggttcat taccgcataa
    55081 ccgacgaagc cagcgaatgc cgctacgcct ttgttttctc gtgccagacc cagcgggatg
    55141 gcgatacaga acatgactgg caggaaacta aaagcaaacg agccaatctt actcatccag
    55201 gtaaagatag cctgcaacac ggggttgccc aggaccggga gcagggtgat gacatcgtgg
    55261 ctactaaggg aactaccaat gccgagcata atgccgcaga acgacaataa tgccacgggt
    55321 aacatgaagg ttttgcctaa ctgctggaag aactcccaca gcgtgacttt cggtgctgtt
    55381 ttcgccgtca taaaacgact cctcgtagag aacaatctgg attcagtaaa ttgcgcgaga
    55441 agataaaacg ttttatcaaa ttttagtgag gcataaatca cattacgcaa cgataatagc
    55501 gggtataaga taaataaaag gtaaaacgtt ttatctgtca caaaatcagg gagtaggtca
    55561 tctgcatggc taccgccaaa aaaataacca ttcatgatgt tgcgctggct gcgggcgtgt
    55621 cggtaagtac cgtttcgctg gtgcttagtg gcaaagggcg aatctctacc gccacaggag
    55681 aacgcgttaa cgccgccatt gaagagctgg gatttgtgcg caatcgccag gcgtcggcgc
    55741 tgcgcggtgg gcaaagcggc gtcattggtt tgatcgtccg tgatttatct gcgccgtttt
    55801 acgccgaatt gacggccgga ttgacggaag ctctggaagc gcagggacgg atggtttttt
    55861 tgcttcacgg cggtaaagac ggcgagcagc tggcacagcg gttttcactg ttactgaatc
    55921 agggtgtcga tggtgtggta attgccgggg ctgcaggaag cagcgatgac ctgcgacgga
    55981 tggcagaaga aaaagctatc ccggtgattt tcgcttcccg tgccagttat cttgatgatg
    56041 ttgatacggt tcgcccggac aacatgcagg ctgcacagtt gttgacggag catctcattc
    56101 gcaatgggca tcagcggatc gcctggctgg gagggcaaag ttcctcatta acccgtgcag
    56161 aacgggtggg gggctattgt gcaactctac taaaatttgg cctgccgttt cacagcgatt
    56221 gggtgttgga gtgcacttcc agccagaagc aagccgcgga agctatcacg gcgcttttac
    56281 gtcataaccc gaccatcagt gccgtggttt gctataacga aactattgcg atgggggcat
    56341 ggtttggttt gctgaaagca gggcggcaaa gcggggaaag cggagtcgat cgttactttg
    56401 agcaacaggt ttcgctggcg gcatttaccg atgcgacacc aaccacactt gatgatatac
    56461 ccgttacctg ggccagcacg cctgcgcggg aacttggtac cacacttgcg gatcgcatga
    56521 tgcaaaaaat cacccatgaa gagacgcatt cacgcaatct tattattccc gcccggctca
    56581 ttgcggcgaa ataatcctct ctttatctgc tatacctggt agtgtccttt cctcaagttt
    56641 aatgataaaa aatgtggcac acaggcggtg ccttttattt ttactgaagt tcacaggagg
    56701 tttatgtgtt taattctgac aacctgagac tcgacggaaa atgcgccatc atcacaggtg
    56761 cgggtgcagg tattggtaaa gaaatcgcca ttacattcgc gacagctggc gcatctgtgg
    56821 tggtcagtga tattaacgcc gacgcagcta accatgttgt agacgaaatt caacaactgg
    56881 gtggtcaggc atttgcctgc cgttgtgata ttacttccga acaggaactc tctgcactgg
    56941 cagactttgc tatcagtaag ctgggtaaag ttgatattct ggttaacaac gccggtggcg
    57001 gtggacctaa accgtttgat atgccaatgg cggattttcg ccgtgcttat gaactgaatg
    57061 tgttttcttt tttccatctg tcacaacttg ttgcgccaga aatggaaaaa aatggcggtg
    57121 gcgttattct gaccatcact tctatggcgg cagaaaataa aaatataaac atgacttcct
    57181 atgcatcatc taaagctgcg gccagtcatc tggtcagaaa tatggcattt gacctgggtg
    57241 aaaaaaatat tcgggtgaat ggcattgcgc cgggggcaat attaaccgat gccctgaaat
    57301 ccgttattac accagaaatt gaacaaaaaa tgttacagca cacgccgatc agacgtctgg
    57361 gccaaccgca agatattgct aacgcagcgc tgttcctttg ctcgcctgca gccagctggg
    57421 taagcggaca aattctcacc gtctccggtg gtggggtaca ggagctcaat taatacacta
    57481 acgaaccggt aaacagccgt atgtgttgtt taccgggata gcgtgtgcaa ttcatcaacg
    57541 tctctgctaa acaactggca gccaaatcac ggccattggt taaccaatag ctggatgaaa
    57601 agcatacgaa tagagtgtgc cttcgcacta ttcaacagca atgataggcg ctcacctgac
    57661 aacgcggtaa actagttatt cacgctaact ataatggttt aatgatggat aacatgcaga
    57721 ctgaagcaca accgacacgg acccggatcc tcaatgctgc cagagagatt ttttcagaaa
    57781 atggatttca cagtgcctcg atgaaagcca tctgtaaatc ctgcgccatt agtcccggga
    57841 cgctctatca ccatttcatc tccaaagaag ccttgattca ggccattatc ttacaggacc
    57901 aggagagggc gctggcccgt ttccgggaac cgattgaagg gattcatttt gttgactata
    57961 tggtcgagtc tattgtctct ctcacccatg aagcctttgg gcaacgggcg ctggtggtcg
    58021 aaattatggc ggaagggatg cgtaacccac aggtcgccgc catgcttaaa aataagcata
    58081 tgacgatcac tgaatttgtt gctcagcgga tgcgtgatgc ccagcaaaaa ggcgagataa
    58141 gcccagacat caacacggca atgacttcac gtttactgct ggatctgacc tacggtgtac
    58201 tggccgatat cgaagcggaa gacctggcgc gtgaagcgtc gtttgctcag ggattacgcg
    58261 cgatgattgg cggtatctta accgcatcct gattctctct ctctttccgc gggctggtga
    58321 caactgagcc cgcgtttcat actttaattt ccctgtacaa aatacaatct cgccggagcg
    58381 gggaattcct ccctaaaata ttcaccgtag ccatatgtca tgagagttta tcgttcccaa
    58441 catgctcgaa cgaacgttcg gttgcttatt ttatggcttc tgtcaacgcc gttttaaaga
    58501 ttaatgcgat ctatatcacg ctgtgggtat tgcagttttt ggttttttga tcgcggtgtc
    58561 agttcttttt atttccattt ctcttccatg ggtttctcac agataactgt gtgcaacaca
    58621 gaattggtta actaatcaga ttaaaggttg accagtattt ttatcttaat gaggagtccc
    58681 ttatgttacg tcctgtagaa accccaaccc gtgaaatcaa aaaactcgac ggcctgtggg
    58741 cattcagtct ggatcgcgaa aactgtggaa ttgagcagcg ttggtgggaa agcgcgttac
    58801 aagaaagccg ggcaattgct gtgccaggca gttttaacga tcagttcgcc gatgcagata
    58861 ttcgtaatta tgtgggcaac gtctggtatc agcgcgaagt ctttataccg aaaggttggg
    58921 caggccagcg tatcgtgctg cgtttcgatg cggtcactca ttacggcaaa gtgtgggtca
    58981 ataatcagga agtgatggag catcagggcg gctatacgcc atttgaagcc gatgtcacgc
    59041 cgtatgttat tgccgggaaa agtgtacgta tcacagtttg tgtgaacaac gaactgaact
    59101 ggcagactat cccgccggga atggtgatta ccgacgaaaa cggcaagaaa aagcagtctt
    59161 acttccatga tttctttaac tacgccggga tccatcgcag cgtaatgctc tacaccacgc
    59221 cgaacacctg ggtggacgat atcaccgtgg tgacgcatgt cgcgcaagac tgtaaccacg
    59281 cgtctgttga ctggcaggtg gtggccaatg gtgatgtcag cgttgaactg cgtgatgcgg
    59341 atcaacaggt ggttgcaact ggacaagggg caccagcggg actttgcaag tggtgaatcc
    59401 gcacctctgg caaccgggtg aaggttatct ctatgaactg tacgtcacag ccaaaagccg
    59461 gacagagtgt gatatctacc cgctgcgcgt cggcatccgg tcagtggcag tgaagggcga
    59521 acagttcctg atcaaccaca aaccgttcta ctttactggc tttggccgtc atgaagatgc
    59581 ggatttgcgc ggcaaaggat tcgataacgt gctgatggtg cacgatcacg cattaatgga
    59641 ctggattggg gccaactcct accgtacctc gcattaccct tacgctgaag agatgctcga
    59701 ctgggcagat gaacatggca tcgtggtgat tgatgaaact gcagctgtcg gctttaacct
    59761 ctctttaggc attggtttcg aagcgggcaa caagccgaaa gaactgtaca gcgaagaggc
    59821 agtcaacggg gaaactcagc aggcgcactt acaggcgatt aaagagctga tagcgcgtga
    59881 caaaaaccac ccaagcgtgg tgatgtggag tattgccaac gaaccggata cccgtccgca
    59941 aggtgcacgg gaatatttcg cgccactggc ggaagcaacg cgtaaactcg atccgacgcg
    60001 tccgatcacc tgcgtcaatg taatgttctg cgacgctcac accgatacca tcagcgatct
    60061 ctttgatgtg ctgtgcctga accgttatta cggttggtat gtccaaagcg gcgatttgga
    60121 aacggcagag aaggtactgg aaaaagaact tctggcctgg caggagaaac tgcatcagcc
    60181 gattatcatc accgaatacg gcgtggatac gttagccggg ctgcactcaa tgtacaccga
    60241 catgtggagt gaagagtatc agtgtgcatg gctggatatg tatcaccgcg tctttgatcg
    60301 cgtcagcgcc gtcgtcggtg aacaggtatg gaatttcgcc gattttgcga cctcgcaagg
    60361 catattgcgc gttggcggta acaagaaggg gatcttcacc cgcgaccgca aaccgaagtc
    60421 ggcggctttt ctgctgcaaa aacgctggac tggcatgaac ttcggtgaaa aaccgcagca
    60481 gggaggcaaa caatgaatca acaactctcc tggcgcacca tcgtcggcta cagccttggt
    60541 gacatcgcca ataacttcgc cttcgcaatg ggggcgctct tcctgttgag ttactacacc
    60601 gacgtcgctg gcgtcggtgc cgctgcggcg ggcaccatgc tgttactggt gcgggtattc
    60661 gatgccttcg ccgacgtctt tgccggacga gtggtggaca gtgtgaatac ccgctgggga
    60721 aaattccgcc cgtttttact cttcggtact gcgccgttaa tgatcttcag cgtgctggta
    60781 ttctgggtgc cgaccgactg gagccatggt agcaaagtgg tgtatgcata tttgacctac
    60841 atgggcctcg ggctttgcta cagcctggtg aatattcctt atggttcact tgctaccgcg
    60901 atgacccaac aaccacaatc ccgcgcccgt ctgggcgcgg ctcgtgggat tgccgcttca
    60961 ttgacctttg tctgcctggc atttctgata gggccgagca ttaagaactc cagcccggaa
    61021 gagatggtgt cggtatacca tttctggacg attgtgctgg cgattgccgg aatggtgctt
    61081 tacttcatct gcttcaaatc gacgcgtgag aatgtggtac gtatcgtggc gcagccgtca
    61141 ttgaagatca gtctgcaaac cctgaaacgg aatcgcccgc tgtttatgtt gtgcatcggt
    61201 gcgctgtgtg tgctgatttc gaccttcgcg gtcagcgcct cgtcgttgtt ctacgtgcgc
    61261 tatgtgttaa atgataccgg gctgttcact gtgctggtac tggtgcaaaa cctggttggt
    61321 actgtggcat cggcaccgtt ggtgccgggg atggtcgcga ggatcggtaa aaagaatacc
    61381 ttcctgattg gcgctttgct gggaacctgc ggttatctgc tgttcttctg ggtttccgtc
    61441 tggtcgctgc cggtggcgtt ggttgcgtta gccattgcct caattggtca gggcgttacc
    61501 atgaccgtga tgtgggcgct ggaagctgat accgtagaat acggtgaata cctgaccggc
    61561 gtgcgaattg aagggctcac ctattcacta ttctcattta cccgtaaatg cggtcaggca
    61621 atcggtggtt caattcctgc ctttattttg ggattaagcg gatatatcgc caatcaggtg
    61681 caaacgccgg aagtaattat gggcatccgc acatcaattg ccttagtacc ttgcggattt
    61741 atgctactgg cattcgttat tatctggttt tatccgctca cggataaaaa attcaaagaa
    61801 atcgtggttg aaattgataa tcgtaaaaaa gtgcagcagc aattaatcag cgatatcact
    61861 aattaatatt caataaaaat aatcagaaca tcaaaggtgc aactatgaga aaaatagtgg
    61921 ccatggccgt tatttgcctg acggctgcct ctggccttac ctctgcttac gcggcgcaac
    61981 tggctgacga tgaagcggga ctacgcatca gactgaaaaa cgaattgcgc agggctgata
    62041 agcccagtgc tggcgcggga agagatattt acgcatgggt acagggagga ttgctcgatt
    62101 tcaatagtgg ttattattcc aatattattg gcgttgaagg cggagcgtat tatgtttata
    62161 aattaggtgc tcgtgctgat atgagtaccc ggtggtatct tgatggtgat aaaagttttg
    62221 gctttgccct cggggcagta aaaataaaac ccagtgaaaa tagcctgctt aaattaggtc
    62281 gcttcgggac ggattatagt tatggtagct taccttatcg tattccgtta atggctggca
    62341 gttcgcaacg tacattaccg acagtttctg aaggagcatt aggttattgg gctttaacgc
    62401 caaatattga tctgtgggga atgtggcgtt cacgagtatt tttatggact gattcaacaa
    62461 ccggtattcg tgatgaaggg gtgtataaca gccagacggg aaaatacgat aaacatcgcg
    62521 ctcgttcttt tttagccgcc agttggcatg atgataccag tcgctattct ctgggggcat
    62581 cggtacagaa agatgtttcc aatcagatac aaagtattct cgagaaaagc ataccgctcg
    62641 acccgaatta tacgttgaaa ggggagttgc tcggctttta cgcgcaactc gaaggtttaa
    62701 gtcgtaatac cagccagccc aatgaaacgg cgttggttag tggacaattg acctggaatg
    62761 cgccgtgggg aagcgtattt ggcagtggtg gttatttgcg ccatgcaatg aatggtgccg
    62821 tggtggatac cgacattggc tatccctttt cattaagtct tgatcgtaac cgtgaaggaa
    62881 tgcagtcctg gcaattgggc gtcaactatc gtttaactcc caatttacac tgacatttgc
    62941 accgattgtg actcgcggct atgaatccag taaacgagat gtgcggattg aaggcgcggg
    63001 tatcttaggt ggtatgaact atcgggtcag cgaagggccg ttacaaggga tgaatttctt
    63061 tcttgctgcc gataaagggc gggaaaagcg cgatggcagt acgctgggcg atcgcctgaa
    63121 ttactgggat gtgaaaatga gtattcagta tgactttatg ctgaagtaaa aaataacgcc
    63181 ggagagaaaa atctccggcg ttttggctgt gaattactgc tgcggaatag cgggtacgac
    63241 cggaacattt aatgccggca ttgcaaacat accaacgaaa tcttccagtg gcattttctg
    63301 cccgtttaac gttatctgac cgttagtata ttgcaggctg gtggtgatgg tattgtcctg
    63361 caaggtggtc agacggaaca tctgccccat tgctgatgca ccttcaactt gctgtttcgc
    63421 cagttttttc gcttgatctt cctgataacc ttccagcttc gctacctgag tcatcaactc
    63481 agttgccata tccaccggaa tggtcagttt cgcatccaga gatttaaccg aacgatctac
    63541 ttcctgcgcc agcgtttgcg gcgcttcttt agtcgttgcc ggatctttca ggaacagcga
    63601 cagattcagc gcactttcac cctgactgtt tttccagctt agcggcgcga tagtaatcac
    63661 cggatcgcct ttcaacatca gcggcagggc gctaaagaag gcttccgtca ctttctcctg
    63721 ataaagttcg ggattgttgg caatttctgg ctgtgccagc agcgcctgag tttgcgcgtt
    63781 atattgctgg ctaaactgat gccaggcttc gccatcaatt tggccgactt ttaaagtcag
    63841 cttgccgctg cccagatcct gattctgtac cttcaggctg tttagcgagt aatccagttg
    63901 gctattgatc gttttaccgt cattgaccag atccgattta ccgctgatct ccatgccttc
    63961 cagcagtgcc agttctttgc cttccactga aatggtcatt ttttccagtg acagtttttg
    64021 gtttcctacg cgctcaccaa aacttgccag cgtgctggaa ccgtcggttt tcagattatt
    64081 aaaggtcaac tgtactttct ggttgtattc gttaaccgcg tctatccgac cactttgcgc
    64141 ctccccggaa agggagatag ctttgccgtc tctgtccgca tttaactgga actcgccgcc
    64201 gctaaaggcg actttttcat ccttttgctc gtaatttagt ggcttgagcg aaatatcgga
    64261 actggaatca ccgctgtaac caatgcgcga gttaatctca aaaggcgttt caccttttgc
    64321 catatcaaac agtggtttgc ttacttcgtt attaaccagc gtggtttgaa ttgatgccat
    64381 cgacgggatc aggttcagtt ttttaagctg ggcaaggggg aagggaccat gatcaaccga
    64441 ttcgttgaag atgacgctct gaccgctttt aatccacgga ttttcttttc cggcaatggg
    64501 tttcaccaac agttgcagct gactgctgaa tacgccgcga tgatagtttt gataactcac
    64561 ttccaggttg gattcaggag cggtcagttt gagttgcgcg ttcgcctgcg cgaccatgtc
    64621 ttcgagatgg gtttcaatct tcttgcctgt ataccatgcg ccgcctgtcc agactacgcc
    64681 tagcgcaaca atgacgccta ccgctaccag cgatttattc ataatgatta tccataaaat
    64741 gaaatcaggc ggactggccg cctgaaggtg ttataagcct ttaataagct tagcaagaga
    64801 tgttaatttt ttcagtaagc tcttacagct tgttgtaaac ccgtgctaaa cggccgtggc
    64861 ctttgacagt caccggtgat tcgttggcgg caataaacgc tgattcaccc ggtttaagct
    64921 gtaactgctg agaacctttc cacaacgttg catcgccttc gacgcagaac aaaatggcgg
    64981 cactctgctg gctaatggtg gtttctttat cactaaggtc atgcagcgag aaggcaaaat
    65041 catcaacagg aatcgggaag tccagttctg caccttgttt caccggctgg gtcaacaact
    65101 ggttagccgg tttggcttcg aacttcacat tggcaaccag ttccggaata tcaatgtatt
    65161 taggcgtcag acccgcacgc agcacgttat cggagtttgc catcacttcc agcgccacgc
    65221 cttgcaggta agcgtgcggt gtttcagcga acaggaacat cgcttcgcca ggatttaatt
    65281 tcaccacatt cagcaacagc ggagagaaca ggccgctgtc atccgggtaa aattcagaaa
    65341 ttaaacgaat cgtttgccac ggttcaccct gctgactgtc gagcgccgat tttaaaatcg
    65401 ccagcgcgcg ggatttttct tcaccctgca tattcaacag gctggcgaac agttcgctta
    65461 aacgttcggc atcaggctgt tgtaaaaagt gagcaatcgc cggatgtgca cctgcgaccg
    65521 gctggagtag ggagacaatc tcggaaaatt cacgaaacgc gttcatcgca aggaaaggcg
    65581 tcagcgcaaa aaccagctcc ggcttgtggt taggatcttt atagttacgc tcggcggcat
    65641 ccatcgggat acctgcggca ttttctttgg caaaaccgat ttcagacttg tgtttgtttg
    65701 gatgaacctg aatggagagt ggctgtgctg cgcataatac tttgaacagg aaaggcagtt
    65761 cgccaaagcg tttggcaacg gcctctccga gcagagtcga tttatcactc tcaatcacat
    65821 cacgcagtga aacgatatct ccggcggcat tctgcactcg tgaactgctt ttcggatgtg
    65881 cgcccatcca cagctcggcc atcggctggc tggacggatt ttccatacca taaagttcag
    65941 tcaacgccgt tttgctgccc caggcatagt tttgcactga gttaatgagt ttttgcatga
    66001 tcaatccctg ttttaatgtg gaaattaatc ccgctattaa agcaagaatc ctacgggaag
    66061 taacctggag ccgcaaaaag tcgtactagt ctcagttttt gttaaaaaag tgcgtaggat
    66121 attgttactc gcttttaaca gggcaacgga acacccgccc agagcataac caaaccaggc
    66181 agtaagtgag aaaacaatgt caaacaaacc ctttcattat caggctcctt ttccactcaa
    66241 aaaagatgat actgagtatt acctgctaac cagcgaacac gttagcttat ctgaatttga
    66301 agggcaggag attttgaaag tcgcacccga agcgttaact ctgttggcgc gccaggcgtt
    66361 tcatgatgcg tcgttcatgc tgcgtccggc gcaccaacaa caggtggccg acattctgcg
    66421 tgacccggag gccagcgaaa atgataaata tgtggcgctg caattcctgc gtaactccga
    66481 catcgcggcg aaaggcgttc tgccaacctg tcaggatacc ggcaccgcga ttattgttgg
    66541 taaaaaaggg cagcgtgtat ggaccggtgg tggtgatgaa gcggcgctgg cgcgcggtgt
    66601 ctataacact tatatcgaag ataatctgcg ctactcgcaa aacgcgccgc tggatatgta
    66661 taaagaagtg aataccggca ccaatctgcc agcgcagatc gatctttatg ccgttgatgg
    66721 cgacgaatac aaattcctct gtatcgccaa aggtggtggt tcggcaaaca agacgtatct
    66781 ctatcaggaa accaaagcgt tactgacgcc ggggaaactg aaaaattacc tggttgagaa
    66841 gatgcgcacg ctgggtacgg cggcctgtcc tccgtatcat attgcgttcg ttattggtgg
    66901 aacttctgca gaaacgaacc ttaaaacggt gaaactggct tccgcgaaat actatgatga
    66961 actgccaacg gaagggaatg agcacggtca ggcgttccgc gatgtggaat tggaaaaaga
    67021 attgctgatc gaagcgcaaa atcttggtct gggtgcgcag tttggcggta aatacttcgc
    67081 tcacgacatc cgcgtgattc gcctgccacg tcacggcgca tcctgcccgg tcggtatggg
    67141 cgtctcctgt tctgctgacc gtaatatcaa agcgaagatc aaccgtcagg ggatctggat
    67201 cgaaaaactg gaacataatc caggcaaata tatcccggaa gagctgcgca aagcgggaga
    67261 aggcgaagcg gtgcgcgttg accttaaccg tccgatgaaa gagatcctcg cacagttgtc
    67321 gcagtatccc gtttctacac gcttatcgct taacggcacg attatcgtcg gtcgtgatat
    67381 tgctcacgcc aaactgaaag agcggatgga taacggtgaa gggctgccgc agtacatcaa
    67441 agatcatccg atttactacg cgggtccggc caaaacgccg gaaggttatg cctccggttc
    67501 tcttggccca acgaccgccg gacggatgga ttcttatgtc gatcaactgc aagcgctggg
    67561 cggaagtatg atcatgctgg cgaaaggcaa ccgcagccag caggtgacgg atgcctgtaa
    67621 aaaacacggt ggcttctacc ttggcagtat cggtggtccg gccgctgtat tggcgcaggg
    67681 aagtattaag agcctggaat gtgttgaata tccggaactg ggaatggaag ccatctggaa
    67741 aattgaagtg gaagatttcc cggcgtttat ccttgtggat gataaaggaa atgacttctt
    67801 ccagcagata caactcacac aatgcacccg ctgtgtgaaa taaacagagc cgcccttcgg
    67861 ggcggttttt ttacatggca cgaaagacca aacatttgtt atcaaatagt aaataatgag
    67921 tgagctaaaa gttacttaac gaaagcaaaa cagaaagaaa aaattaatca ggtgaggagt
    67981 aggccatgaa tacagtacgc agcgaaaaag attcgatggg ggcgattgat gtcccggcag
    68041 ataagctgtg gggcgcacaa actcaacgct cgctggagca tttccgcatt tcgacggaga
    68101 aaatgcccac ctcactgatt catgcgctgg cgctaaccaa gcgcgcagcg gcaaaagtta
    68161 atgaagattt aggcttgttg tctgaagaga aagcgagcgc cattcggcag gcggcggatg
    68221 aagtactggc aggacagcat gacgacgaat tcccgctggc tatctggcag accggctccg
    68281 gcacgcaaag taacatgaac atgaatgaag tgctggctaa ccgggccagt gaattactcg
    68341 gcggcgtgcg cgggatggaa cgtaaagttc accctaacga cgacgtgaac aaaagccaaa
    68401 gttccaacga tgtctttccg acggcgatgc acgttgcggc gctgctggcg ctgcgcaagc
    68461 aactcattcc gcagcttaaa accctgacac agacgctgag tgaaaaatcg cgtgcatttg
    68521 ccgatatcgt aaaaatcggt cgaacccact tgcaggacgc caccccgcta acactggggc
    68581 aggagatttc cggctgggta gcgatgctcg agcataatct caaacatatc gaatacagcc
    68641 tgcctcacgt agcggaactg gctcttggcg gtacagcggt gggtactgga ctaaataccc
    68701 atccggagta tgcgcgtcgc gtagcagatg aactggcagt cattacctgt gcaccgtttg
    68761 ttaccgcgcc gaacaaattt gaagcgctgg cgacctgtga tgccctggtt caggcgcacg
    68821 gcgcgttgaa agggttggct gcgtcactga tgaaaatcgc caatgatgtc cgctggctgg
    68881 cctctggccc gcgctgcgga attggtgaaa tctcaatccc ggaaaatgag ccgggcagct
    68941 caatcatgcc ggggaaagtg aacccaacac agtgtgaggc attaaccatg ctctgctgtc
    69001 aggtgatggg gaacgacgtg gcgatcaaca tggggggcgc ttccggtaac tttgaactga
    69061 acgtcttccg tccaatggtg atccacaatt tcctgcaatc ggtgcgcttg ctggcagatg
    69121 gcatggaaag ttttaacaaa cactgcgcag tgggtattga accgaatcgt gagcgaatca
    69181 atcaattact caatgaatcg ctgatgctgg tgactgcgct taacacccac attggttatg
    69241 acaaagccgc ggagatcgcc aaaaaagcgc ataaagaagg gctgacctta aaagctgcgg
    69301 cccttgcgct ggggtatctt agcgaagccg agtttgacag ctgggtacgg ccagaacaga
    69361 tggtcggcag tatgaaagcc gggcgttaat ctgcaacata caggtgcagc cgtggaatga
    69421 tcaaacgaag cggctgcgcc tgaggtttat aacggtgctg cacattgtcg gcatcgtaat
    69481 ttaacaactc accaacgtcc ggcacgcccg caccattatc ccgattaatc agtgcaatca
    69541 gcggtgtagg gcaggcgtgt tggacttgtt tttgatctcc tttgtaccag acgcgggcaa
    69601 tcggctgcac cttcaccgga cgtttgattt ttaacttcgc attctgtggc agggcagcga
    69661 tatcctgata ctctcgctcc agttttcttt gccactcctc gcgcgtccac ggtgcgacac
    69721 tgcgtggtga tttcaggctt ttttccagct gtgccaggac ttcatcacga tgcaaattct
    69781 taatgatatg tttattagcc caaccaaagc gtaaagtggc gggatcgtgc agaacggtga
    69841 gcgtgcggta agcattaagg gtgatcagcc ccggcaaatg acgatgcacc cattcaaaac
    69901 gtgccgcggt ggggagttct gattcaaccg tgacgatatg ctcgaacgtg gtcttgagtt
    69961 tattgatgtg ctgaatatga ctgaccaacg ctgcttgcga aaggttatcg acctggtaac
    70021 acaacacgcc aggcagacgg acagctgctt tgctgctgcg attttcggac tgttgttgaa
    70081 taaataaatg acggaaatga cgcaacgcct gcgactgcgc gtcgttgccg agatgttgtt
    70141 tcacctcaat acgattaagc ggattatgct catcctcttt ttttacctcc ggcaaagaga
    70201 acacgcgggc aaccaatagc ttgtgttgct caagatgagc ggcaaatgca gccagctctt
    70261 gttccatctg gcgaaaagta gtgttgagtc ggtctacgag atcgtaacgc gccataatat
    70321 taaccacttt agttacaaca tccttaattt atagcacagt ggtggtgact tgaccaccgt
    70381 gcgcgtagtg tcaagcagag gtaaattgcg ggatgttatg ccataacggc cagctaaacg
    70441 agaagcgggc accacccagt tcgctggtgt cacaattaac cgtaccgccc attgccagtg
    70501 ctatagagtg gacaattgcc agccccagcc cgcagccgcc ggttgagcga tcccggctgg
    70561 gatcgaggcg aacaaaaggt tcaaagatat gttcgcggtt ttctggggca atccctgggc
    70621 catcatcctc aacaattaat gtcgctctat tccccgacag tagcaggctg gtttcaaccg
    70681 ttgaatggca gtagcgcagg gcattattga gcaaattatc cagcacgcgc tccattaagc
    70741 gcatatccaa cgccgcataa tggccttgca tgagcgtttt tatccgtacc gttttatcgg
    70801 gcgttactgc ctgaatatct gccagatgcg ttgacagcca caacggcagg tctggttcgc
    70861 taagatgaag ctcgttttgt gggcgatcga gtcgggcata agtcagcagc tcttcaatta
    70921 aagcttcaag ttgactgata tcacgattca acgcctggga ttcggcggcg ctcaggttat
    70981 cgctcatctc cagtcgataa cgcaggcgca ctaacggtgt tcgcagttcg tgagcgatac
    71041 cgtcaataag ctgttttttg ctggcaatca aggcgttgat attgtccgcc atctggttaa
    71101 atgcgatacc aagtcgttca aagctcgaac cctcatcaaa gtggatacgt tcattgagat
    71161 gcccatcgcc aaatcgttgc gccgctgctt ccagttttaa catatcctgc cagtgcggac
    71221 gcatccagat aaacaccgga aaggcgaggg aaatagcaat aaaagcgatc agggcgatat
    71281 ccagcaatcg catctgatgg agataataaa gataaggaac aggaccaact gccagcacgt
    71341 agtggctgcg cgggatacgc tgcaaaaaag tgtactgatc gtccagggcg acaatttcgc
    71401 cgccacgcag ccggtgcatg gaaatatcat caagatggta tttactcagt ggctcgactc
    71461 gcagatcgaa agagagattt aaatccatct ctttcagcgt cttaccccag tcatgtgggg
    71521 ggatctcacg caattcgctg cgcatcagat acagcgaact gttcatcaaa tcatccagcg
    71581 actgtttgcc cgcgcgttcg gcggtaaatt tgtacaccaa cccaaccagc agagacatca
    71641 caaggaagca gacaaacaat aacaggtaaa actggataaa cagttttttc atcgcttatt
    71701 cccatgcatg aggcgcaaaa agatagcctt tgttacgcac agttttaatg cgataaggtt
    71761 cagcggcgtt atcgagcagt ttttttctta accgcgaaat agccacgtcc acgctacgat
    71821 ccaggccgtc ataactgacg ccgcgtaaat ttttcagcaa tgcatcgcgg tccatgattt
    71881 gcccggcatg ggtagctaat tcccacaata attcgaaatc agctgtcgag agcgagattt
    71941 cagtgttagc cagggtgact acgcggttga taggatcgat ggtcaacgtg ccgaaatgca
    72001 gggctttgta gggagtcagt gacgtttcct gaagaccttt ggtcagcgtg gcttgctcat
    72061 tctgacgcaa atgcaaacgt aaacgcgcta acaaaacagc agggggcgtc gttttgagaa
    72121 tatagtcgca ggcacccatt tccagtgcca ggatgtggtt catatcgcta tcgagagagg
    72181 ttagaagaac aatcggtccg gaccactttg cgcgtaaatc acgacaaatg gtcatgccgt
    72241 ccttgcctgg tagcatgatg tcgagtaaca ccaaatccgg attttctcgc aaaatggttt
    72301 cttcggcctg gtcgccgcgc ggctctacgg taacctgtat atcatgtttt gccaggtacg
    72361 cggcaatcag tgaaccgact tccgcatcat cttccacaaa tacgatagtg ttcataacat
    72421 tcacggtaga tataaaaacg tcacaataca ccgcgctgtt tttacttact atcaatcgtt
    72481 tctaaacaag ttttcatcag gtattctact gatgacttgt tatggaagtg ttaaggtaaa
    72541 aagatggggc tggtaatcaa agccgcgctc ggtgcgctgg tagtgctgtt gattggtgtt
    72601 ttagcaaaaa cgaaaaatta ttatatcgcc gggctgattc cacttttccc gacctttgcg
    72661 cttatcgcgc attatattgt tgccagcgaa cgcggcattg aagccttacg cgcaaccatc
    72721 atttttagta tgtggtcgat tattccctat tttgtttacc tggtgtcgct gtggtatttc
    72781 accggaatga tgcgcttgcc cgccgctttt gttggttctg ttgcgtgttg ggggataagc
    72841 gcatgggtgt tgattatatg ctggataaag ctgcattaac gcagatgacg accgccatca
    72901 agtgggaaac tgcgtccggt gacaaagcaa ctggtaagta agtaatcgac caggtcgatc
    72961 acttctttct cgccaggcgc ggttttcatc aatgatttat tcagcgcctg ttgtcgatat
    73021 tcggcatcat catgttcatt aaacaggatc agcgatggcg caatagaatt cactttcact
    73081 tccggtgcca gcttgcgggc aaacgagcgg gtcatattat ccagtgccgc tttgcttgca
    73141 gcatacgcaa tatgtttgtc gctaccgcgc tccaccacat aatcggtaaa gtgaatgata
    73201 tcgctggcgg cgtgtccgtg cccacgcagt aatctttcca gcgcatggtt gagcaggtat
    73261 ggggtattaa cgtggatctg catcatgcaa gccagtacgt cggccagtgg cgcacccggt
    73321 ttttccgcca tccacgcact ggcgttatgc aaaatagcac gcagaccatg ggtgcttttt
    73381 agtacttcat cggcaaacgc catcacaccg tcgttggtcg aaaaatcagc ctgaatacac
    73441 tgcgcacctg cattaatcag tccatcaatg gctggatagt gtgtccgata gctgacaatc
    73501 accggttgct tttgattaat gaaatgccat gcgagggcga ggccgatgcg acgacctccg
    73561 ccagtaatta atattggcaa gggctgggtt ttacccatcg ttatctcctt tgctatccaa
    73621 cgatgggatg aacgttatcc caccagcatc caggtcgccg gaactgaggc aattaacagc
    73681 atgccaatca gcaccatttc ctgacggttc agtacattat catgtgtatg cgttttgcgc
    73741 gcatacagga aaaccagcag ccccggtgca tacagaacaa cggataacag caagtgcatt
    73801 ggaccagagg cgtacaataa ccataagcca taaatgcagg caccgacacc taccgcttta
    73861 tgcagtggac gggttgcgat tttcagcaag aacgcgccga ccaggaaata gggcaccaga
    73921 atcatttctg aggcgatggt cagcaacgtg ttgtaatcgg aaccggtcag ccagatgagt
    73981 accagacaaa tttgcacgca gatgtttgtc aaccacagtg aggcggatgg cgcagcttgt
    74041 gcgttctggc gcgcaaaaat gcgtgggaat gctttatgag tggcagccag gaacggaact
    74101 tctgccgcca tgatggtcca gcttaagtac gcgccgcaaa cggaaacgat caaaccggca
    74161 gcgatgatga tttcgcccca cggtcccatc atttccacca tcagaccggc cattgacggg
    74221 ttacgaattt cagccagttc aggacgtgcc accactccca gcgaaagcag cgttaccagc
    74281 aagtaaacgc ccagagcgga gagaaccgcc agcagtgtcg ctttgccaac atcacgttta
    74341 ttacgcgcac gcgcagaaac aaccacggca ccttccacac caatgaaaac ccacagggtg
    74401 atcagcatgg tgtttttcac ctgttcccaa acaggtacgc caagtgcaag tccggtgaag
    74461 tcgagcttga agatatccag tttgaacatc atcatcgcca gcacaacaaa cagacccagc
    74521 ggcaacaatt ttgccagtgt cgccaccagg ttaatgctgg cagcggtttg caccccgcgc
    74581 aggatcaaaa agtgaacaat ccacaataaa gccgatgcac cgactatcga ttgccaggta
    74641 ttaccatcgc caaataggcg caattccggc gtgtccgtaa aaaagcttaa cgcggaaaaa
    74701 acgatcacca gataggagac gttggcgatg actgcgcaca gccagtatcc ccatgcggaa
    74761 caaaagccga ttagctcgcc aaacccttcg cgggcatagg taaagatacc gccgtcaagt
    74821 tcgggacgaa tgcgcgtgag gatcagcatg gcaaaggcca gcaataaaat gccagcgcca
    74881 gtaatacccc agccgatgag cagtgctgcc gggctggcaa ctgccgccat attttgcggc
    74941 agactgaaaa cacccgcgcc cagcattgag cttaatacca gcgcggtgag tgcgctcagt
    75001 cccagtttct tttccatcga tatccagtga ttaaaacgtg ataagaagaa taaatattag
    75061 ggcaaaacgc ataaaaatgg agtttgccac tgaagcgcgg gattttacga agagatgtgt
    75121 ctgcatgcaa tggtcgcggg gaaatttgtg actaaaaatg cgaaaggcgg catcaagccg
    75181 ccttataaga gtaactgaat agttatttat acagatctgc actgacggtc aggttattac
    75241 cacgttcctg ccactggcgg gtgatgtggt aatatttagc acctttcttc gcggctcgtt
    75301 tcgcaacctg ataggagact tcggtcatgt tgccgtagtt gccagagaat ttgatgctgt
    75361 cgaacgggac catcatcgct gctgtcgctt tgttcagttc ttcgacttta gtgccatccg
    75421 gaagcgtgac agtgtaacgc ccgccttttg atgactgggt ttcaaagaag cgaccgactt
    75481 cagaacttgg tgatgcggta gtcgcaacac ccgggatctc aactttcttc gcggcttcgc
    75541 caccggcagc cagagctgca cgtcctgctt cggaatctgc cgggatcaca tccgggctct
    75601 ggacgatacg tttcttagca tcttttttat agatgaatgc agtaatacgc tggttgccgc
    75661 cctggttggc atcgatttga cgaacgatgt agaaagagta ggcacctttc gcttttgccg
    75721 ctttggtgat ggcatcattg acttctggct ggctacgata gaagccctgg acggtgaccg
    75781 tgtcaaacgg ttcaatcaga acagcctgat ctttcggcag ttcgacaaca ccgttaatta
    75841 cgcgattact tgtttcttct gctttttcag catcggcttt atagaggtca gcgaccacac
    75901 gccagttacc gctgttccca aaatcagaag tgtcgacaac ataaaaagag gcggcacctt
    75961 ctttatctgc gcgacgagaa acggctttca ccgcttcgcc aatagcatta aaacgaccgg
    76021 taaccactac acggtcaaaa ggtttaaccg ctgccgcttg ctccggtgtc agttctgttg
    76081 ctgcgttaac ggagaatgcc gtagcagaaa gcagtgccga cgccaggagg gtgttcttaa
    76141 gcttcataaa aataatcctt cgccttgcgc aaaccaggta ctggtattgt tattaacgag
    76201 aaacgtggct gattattgca tttaaacggt gtaactgtct gcgtcatttt tcatatcaca
    76261 ttccttaagc caattttaat cctgctcaaa tgaccgtcta tgcttaaaaa acagccgtat
    76321 cagcatcatt actactgaag caactgaatt gtataagtta atttaatgtt aagtagtgat
    76381 tcgtgccggg gcgatgtctc gttttacccg accgtcgaag acaattatca gtctttatcc
    76441 ggcgttctaa ggtgtttatc ccactatcac ggctgaatcg ttaatatttt gcgagttcac
    76501 gccgaaatac tgatttttgg cgctagatca caggcataat tttcagtacg ttatagggcg
    76561 tttgttacta atttatttta acggagtaac atttagctcg tacatgagcg gcttgtgtgg
    76621 ctcctgacac aggcaaacca tcatcaataa aaccgatgga agggaatatc atgcgaattg
    76681 gcataccaag agaacggtta accaatgaac cccgtgttgc agcaacgcca aaaacagtgg
    76741 aacagctgct gaaactgggg tttaccgtcg cggtagagag cggcgcgggt caactggcaa
    76801 gttttgacga taaagcgttt gtgcaagcgg gcgctgaaat tgtagaaggg aatagcgtct
    76861 ggcagtcaga gatcattctg aaggtcaatg cgccgttaga tgatgaaatt gcgttactga
    76921 atcctgggac aacgctggtg agttttatct ggcctgcgca gaatccggaa ttaatgcaaa
    76981 aacttgcgga acgtaacgtg accgtgatgg cgatggactc tgtgccgcgt atctcacgcg
    77041 cacaatcgct ggacgcacta agctcgatgg cgaatatcgc cggttatcgc gccattgttg
    77101 aagcggcaca tgaatttggg tgcttcttta ccgggcaaat tactgcggcc gggaaagtgc
    77161 caccggcaaa agtgatggtg attggtgcgg gtgttgcagg tctggccgcc attggcgcag
    77221 caaacagtct cggcgcgatt gtgcgtgcat tcgacacccg cccggaagtg aaagaacaag
    77281 ttcaaagtat gggcgcggaa ttcctcgagc tggattttaa agaggaagcg ggcagcggcg
    77341 atggctatgc caaagtgatg tcggacgcgt tcatcaaagc ggaaatggaa ctctttgccg
    77401 cccaggcaaa agaggtcgat atcattgtca ccaccgcgct tattccaggc aaaccagcgc
    77461 cgaagctaat tacccgtgaa atggttgact ccatgaaggc gggcagtgtg attgtcgacc
    77521 tggcagccca aaacggcggc aactgtgaat acaccgtgcc gggtgaaatc ttcactacgg
    77581 aaaatggtgt caaagtgatt ggctataccg atcttccggg ccgtctgccg acgcaatcct
    77641 cacagcttta cggcactaac ctcgttaatc tgctgaaact gttgtgcaaa gagaaaaacg
    77701 gtaacatcac tgttgatttt gatgatgtgg tgattcgcgg cgtgactgtg atccgtgcgg
    77761 gcgaaattac ctggccggca ccgccgattc aggtatcagc tcagccgcag gtggcacaaa
    77821 aagcggcacc ggaagtgaaa actgaggaaa aatgtgcctg ttcaccgtgg cgtaaatacg
    77881 cgttgatggc gctggcaatc atccttttcg gctggctggc aagcgttgcg ccgaaagagt
    77941 ttcttgggca cttcaccgtt ttcgcgctgg cctgcgttgt cggttattac gtggtgtgga
    78001 acgtatcgca cgcgctgcat acaccgttga tgtcggtcac caacgcgatt tcagggatta
    78061 ttgttgtcgg agcactgttg cagattggcc agggcggctg ggttagcttc cttagtttta
    78121 tcgcggtgct tatagccagc attaatattt tcggtggctt caccgtgact cagcgcatgc
    78181 tgaaaatgtt ccgcaaaaat taaggggtaa catatgtctg gaggattagt tacagctgca
    78241 tacattgttg ccgcgatcct gtttatcttc agtctggccg ggctttcgaa acatgaaacg
    78301 tctcgccagg gtaacaactt cggtatcgcc gggatggcga ttgcgctaat cgccaccatt
    78361 tttggaccgg atacgggtaa cgttggctgg atcttgctgg cgatggtcat tggtggggca
    78421 attggtatcc gtctggcgaa gaaagttgaa atgaccgaaa tgccagaact ggtggcgatc
    78481 ctgcatagct tcgtgggtct ggcggcagtg ctggttggct ttaacagcta tctgcatcat
    78541 gacgcgggaa tggcaccgat tctggtcaat attcacctga cggaagtgtt tctcggtatc
    78601 ttcatcgggg cggtaacgtt cacgggttcg gtggtggcgt tcggcaaact gtgtggcaaa
    78661 atttcgtcta aaccgttgat gctgccaaac cgtcacaaaa tgaacctggc agctctggtc
    78721 gtttccttcc tgctgctgat tgtatttgtt cgcacggata gcgtcggcct gcaagtgctg
    78781 gcattgctaa taatgaccgc cattgcgctg gtatttggct ggcatttagt cgcctccatc
    78841 ggtggtgcag atatgccggt ggtggtgtcg atgctgaact cgtactccgg ctgggcggct
    78901 gcggctgcgg gctttatgct cagcaacgac ctgctgattg tgaccggtgc gctggtcggt
    78961 tcctcggggg ctatcctttc ttacattatg tgtaaggcga tgaaccgttc ctttatcagc
    79021 gttattgcgg gtggtttcgg caccgacggc tcttctactg gcgatgatca ggaagtgggt
    79081 gagcaccgcg aaatcaccgc agaagagaca gcggaactgt tgaaaaactc ccattcagtg
    79141 atcattactc cggggtacgg catggcagtc gcgcaggcgc aatatcctgt cgctgaaatt
    79201 accgagaaac tgcgcgctcg tggtatcaac gtgcgtttcg gtatccaccc ggttgcgggg
    79261 cgtttgcctg gacatatgaa cgtattgctg gctgaagcaa aagtaccgta tgacatcgtg
    79321 ctggaaatgg acgagatcaa cgatgacttt gctgataccg ataccgtact ggtgattggt
    79381 gctaacgata cggttaaccc ggcggcgcag gatgatccga agagtccgat tgctggtatg
    79441 cctgtgctgg aagtgtggaa agcgcagaac gtgattgtct ttaaacgttc gatgaacact
    79501 ggctatgctg gtgtgcaaaa cccgctgttc ttcaaggaaa acacccacat gctgtttggt
    79561 gacgccaaag ccagcgtgga tgcaatcctg aaagctctgt aaccctgacg gcctctgcgg
    79621 aggccgtcac tctttattga gatcgcttaa cagaacggcg atgctttgac ctcccgctgt
    79681 ttgttcaagc gcaattttga caataattgt taacggcacg gaaagcagca tacccaccgg
    79741 tcctaacaac catccccaaa aaatcaacga caaaaatacc accaatgtgg aaagtcccag
    79801 cccacgcccc atgatgcgtg gctccagaat attgccgaag accagattaa tcagcagata
    79861 tcccgccagc accaacaacg cttcgtagaa gccattaaac accagtacct gagcaatagg
    79921 ggggattgcc gcgagaactg aaccaatatt agggatgtaa ttaagcgcaa aggccagcaa
    79981 tccccagaca aaagcgaagc gaacatcgag ggcggcgagc atcgcccatg cgaccaggcc
    80041 ggtgatgatg ctgatggctg ttttcagcac cagataatga gacacactgt caatcgcacg
    80101 ctgaatcgcc gccatccctt caaccggacg cgccatcatt tgcttaaatt ttccgggcaa
    80161 ttgtggcact tcgagcagca taaacagcac cgtcagcagc aataaaaata ttgatgacat
    80221 ggcattagat aactgcgtca ataagttggt gagcaacgtc atcgccgcgt tcggatcaat
    80281 gtaatgcgcc agttggtcaa ctgagacgtc aatccctacg cgttgcaaca acggctcaag
    80341 agcttgcagc ggcgtcataa tagagttgcg atattgcggt aacgtccgcg tcaactcgtt
    80401 gagcgtggaa cccagataag ctaacagcaa caccatagcc atcacgatga tggtcatcaa
    80461 aatcgacact gccagtacac gcggcacacg ccagcggacc atgtgttgca ccagcgggtt
    80521 aagaataaca gcaataaata atgcgagaat aaacggcacg atgatctcgg cggcaaaacg
    80581 gataccgcag agaataatga ccagcattcc caacataatg acgattttta ggccattgag
    80641 cgtgatgagc ggctttgcca tgcttgtccc cgttttttct tcctttgcta ataataatgt
    80701 ctttttcgct ccgtcatcat aacgaaaatc aaaccgctca ggtaaagaag tgaaaatatt
    80761 ttgagttaat tcttaagcta tgatacaaat caggcgtgtt caactaccga ggacaattat
    80821 catccgcgat gacgagaagc aacactgcgg ataattgtaa tattatggac aatttgttca
    80881 ggacgttatt ttctacattt acccatcttc gtacctcatc cactatcttg ctcgtaggtg
    80941 agcagcactg gcgcaacgcg ctttagtcct ttcttctgcc taagcttgcc ctatacgcgg
    81001 ggcttgacca ctttactttg gtttcgctga attaagcgaa aattaaaata attctcttgc
    81061 aggagaagga caatgtatat ttattggatt ttattaggtc tggctattgc tacagaaatt
    81121 accggtacgc tgtctatgaa atgggcgagc gtcagtgagg gaaatggcgg ctttatttta
    81181 atgctggtga tgatttctct gtcgtatata tttctctctt tcgctgttaa aaaaatagcc
    81241 ttaggtgtgg cttatgcgct gtgggaaggt atcggtattt tatttattac cttgtttagc
    81301 gttttgttat tcgacgaaag tttatcgctg atgaaaattg ccgggttaac caccctggtc
    81361 gccgggatag tgttgatcaa atcaggtacc cgtaaagcac gtaaacctga actggaggtg
    81421 aaccatggcg cagtttgaat gggttcacgc cgcctggctg gcattggcaa tcgtgctgga
    81481 aatcgttgct aacgtctttt tgaaattttc tgacggcttt cgtcgcaaaa tatttggctt
    81541 gctctccctg gcggcggtgc tggctgcctt tagtgcgctt tctcaggccg ttaaagggat
    81601 cgacttgtct gtcgcttatg cattgtgggg cgggtttggt attgccgcca cgttagccgc
    81661 aggttggatc ttgtttggtc aacggttaaa tcgtaaaggc tggattggcc tggtcttgct
    81721 gttggctgga atgatcatgg tgaaacttgc ctgatgaaga cgctgcccgc gctgtcgggc
    81781 agcgtttgaa cattattttt gcgacagttg atccagcttg tcgcgaaaac cggtaacaga
    81841 aatggcccgg ttatcggcgc gccagcgatc tttcgcggca ggagccgaac tttgcacccc
    81901 aattaattgc cagccgtcat cggtatgcaa catcagaggc gaaccgctgt cacccggcaa
    81961 ggtatcgcac tgatgtgaca tcaccgacgt ttgcgcccag ccagtcactt cacagttttg
    82021 atgactgtac aacgtatcga gatgatcttc agggtatcct gcctgagtca ctttacgacc
    82081 tgccgctttt aatgcggcgg taagcgcggc tttatctccc tcaaataacg gcaacggcgt
    82141 aatgccagaa gggggattac gtagcacaat caatccgaag tcccacggcg cggctgcggg
    82201 aggtacaatc caaccatccc catctgcttt taaccgcttt cccagtgtcg gatcaacgcg
    82261 gccttctatg tcgtggatgt catagcgcca aagaccttta tttgacacaa aacgcagcgc
    82321 cactgcttta tcggctttac cctttggtgg cgtcaataaa cagtgtcctg ccgttaatgc
    82381 cagattgggt gcaatcagcg tcgccgtaca taaattgccg ctggccgttt ccagttgccc
    82441 aaccgcatcc cacggtgatt gggtcgtgtc attcactggc acacgatcat catgaccaaa
    82501 aaacagggtg ctgacctcat cgtttgccga tttggcaacg tctggtttat ctgcaaacac
    82561 aaaagcagac gtcaaactaa ttgcacccaa cactacagca atggttgtac gcatatcaca
    82621 ctctggtggg taattatgat tattaaaagc aaaccctcat aaatactata gacgggacgg
    82681 gatgaaagtg ggagtaaaat cagataacta cgatcaggaa agataaaaac gggcggcaat
    82741 cagtgcgcat aaaataagga tgataagaat gaactcaaag cgataacggc ccaccatact
    82801 accctccgct aaaggcggtg ctaaacgtta cccgcttagc accgtaattt tcaggcgcga
    82861 gggggcgcgc cagcattact gttgaaaact tacgctgcgg gttgtgcagc aggttttgcc
    82921 ggttgctgat ggctgtgttt cttggcgtgt ttcttcgctg cctgcgcttt ttgttctggg
    82981 gctttctgtt cagctttcgt atttttatga tgctttttag ccgcctgcgc tttctgggca
    83041 ggtgctgctt tatgctgttt tttatgatgt gtagttttcg ccggcgctgc tttggtggtc
    83101 gtcgcagtcg gagcaggtgt ggtcgcagtc tctgcagcaa aggcggcaga agacagaccc
    83161 atagcagcgg caacaaccag agctaatact tttttcattg tcataccctc aatttgtttt
    83221 ttcatttaac cccactgcgg ggccgttgaa agaactatat ccctgggaat ggcagacttc
    83281 cgtgagtggt tggtttcagc gtgtaacgat atgtacaaac gctgaataaa ttacagcgtt
    83341 gataataacg tattgtgact taaggaaaat ttagctacca ataatagtag tcttgatcgg
    83401 gttaactatt ttactactta caaagcggta gaataactgc gcatcagtaa taaatgacac
    83461 acagcaaaat gaatccgttt atttgggtac ttataatcct gatgacgcta gacgcgctgc
    83521 gggaattggc tggcgcttcg tctattttag gatggctatt aacgttggtt tgagcaggca
    83581 ataagtccgg acgggtattt accgcagtcc ggacttattt ttcaggcgtg cagacgacga
    83641 tgcaaacgcg tcccgaccag cagagcaatg accagcatca gagcaataaa tgcgccgacg
    83701 ccgttccagc catagttatg ccagaaaaca ccacccagcg tcccggcaat actcgacccc
    83761 agatagtaac tgaacagata cagcgaggag gcctggcctt tagcgcgttt tgcgcggggg
    83821 ccgatccagc tgctggcaac tgagtgggct gcgaagaatc ctgctgagaa gagtaacatt
    83881 ccggcaaaga tcagccacag cgagctgaat aaggtcatca gtaaaccaaa cagcataacc
    83941 cccgtcgaaa acaacatcac tggaccacgc ccatagcggg tggtcatggt tccggctttg
    84001 ggtgagctcc atgtaccggt caaataagcc agcgataata agccaaccac ggcctgactg
    84061 acatgccagg gggagagcat caaccgatag ccgatgtaat taaacagcgt gacgaacgac
    84121 cccatcagca aaaagccttc tgcgaacaat aacggtaatc cccggtcacg ccagtgcaga
    84181 cgaaagttga taaacaacga cttaggtcgc agcgaagtcg ggcgaaaatg gcgtgattcc
    84241 gggaggattt tccagaacat caacgccgag gctagcgcga aacaaccgat agccgccaga
    84301 gcaattcgcc agttgaaaaa gtccgtgaag acaccactaa ttaagcgtcc gctcatgccg
    84361 ccaattgagt tgccgctgat atacaacccc atcgaaaagg ccacgaaact ggggtggatt
    84421 tcctcgctaa gataagtcat gccaacagct gccacgccac ttaacgaaag cccaatcaag
    84481 gcgcgcataa tcaaaatgcc gtgccagctg gtcatcattg tcgaaagtaa cgtacaaatg
    84541 gaggccaata gtagcgccgt taccatcact ggtttgcgac caatggcatc ggataggggg
    84601 ccagtaaaca gcaaaccaat agccaacatc gccgtggaaa tggacagtga aatactactg
    84661 ttcgcgggcg ttaagccaaa ctcctgcgaa agcaccggaa ggataggttg cacacaatag
    84721 agaagtgcaa atgttgccag tccggcagag aacagcgcca gggtgacgcg cataaattgc
    84781 ggcgtaccgc gtttaataaa ttgatttggc tgagaaatgc tttgcttgtc agtgtcgctt
    84841 gccggagcgc catcaacagt tgtagtacgg ctcacttgaa atccttgcta aatatgcctg
    84901 tggatcaggc ttatacatag gttaggaaaa tcgaattgtt ctgtctaata tattaataat
    84961 ctcaaataag atgttttaaa tatgaatatt gaacttcgtc atctgcgtta ctttgttgct
    85021 gttgcggaag agctgcattt cgggcgcgcc gctgcccgcc tgaatatttc gcaaccgccg
    85081 ctaagtcagc agattcaggc gctggagcaa caaattggtg cccgactgct ggcacgaacc
    85141 aatcgcagtg tattgctgac ggcagcagga aaacagtttc ttgcagatag tcggcaaatc
    85201 ctgtctatgg tggatgacgc tgccgctcgc gctgaaagac tgcatcaggg tgaagcgggg
    85261 gagttgcgca ttggttttac ttcgtcggct ccttttattc gggcggtgtc cgatacgtta
    85321 tcgctgtttc gccgagatta tcccgatgtc catttacaaa cccgcgaaat gaacactcgc
    85381 gagcaaatcg ctccgctcat tgaaggaacg ctggatatgg gattgctgcg taacacagcg
    85441 ttaccggaga cgcttgaaca cgcagtcatc gtccatgaac cgcttatggc gatgatcccg
    85501 cacgatcatc ccctggcaaa taacccgaat gtaacgctgg ctgaactggc gaaagaaccc
    85561 tttgtctttt ttgatccgca cgtcgggaca gggctgtatg acgatattct cgggctgatg
    85621 cgacgttacc atttgacgcc cgtcatcact caggaggtgg gcgaggcaat gaccatcatc
    85681 ggtctggttt ccgccggtct gggtgtttca attttgcctg cgtcatttaa acgtgttcag
    85741 ctcaacgaaa tgcgctgggt gccgattgct gaagaggatg cggtttctga aatgtggttg
    85801 gtctggccga aacatcatga acaaagtcca gctgcgcgta actttcgtat tcatctgctg
    85861 aatgctctca ggtgagggaa atttcagcga aaaagcccga aaaatgtgct gttaatcaca
    85921 tgcctaagta aaaatttgac gacacgtatt gaagtgcttc accatagcct acagattatt
    85981 tcggagcgcg aaaatatagg gagtatgcgg tggttgctga aaaccagcct gggcacattg
    86041 atcaaataaa gcagaccaac gcgggcgcgg tttatcgcct gattgatcag cttggtccag
    86101 tctcgcgtat cgatctttcc cgtctggcgc agctggctcc tgccagtatc actaaaattg
    86161 tccgtgagat gctcgaagca cacctggtgc aagagctgga aatcaaagaa gcggggaacc
    86221 gtggccgtcc ggcggtgggg ctggtggttg aaactgaagc ctggcactat ctttctctgc
    86281 gcattagtcg cggggagatt ttccttgctc tgcgcgatct gagcagcaaa ctggtggtgg
    86341 aagagtcgca ggaactggcg ttaaaagatg actcgccatt gctggatcgt atcatttccc
    86401 atatcgatca gttttttatc cgccaccaga aaaaacttga gcgtctaact tcgattgcca
    86461 taaccctgcc gggaattatt gatacggaaa atggcattgt acatcgcatg ccgttctacg
    86521 aggatgtaaa agagatgccg ctcggcgagg ctctggagca gcataccggc gttccggttt
    86581 atattcagca tgatatcagc gcatggacga tggcagaggc cttgtttggt gcctcacgcg
    86641 gggcgcgcga tgtgattcag gtggttatcg atcacaacgt gggggcgggc gtcattaccg
    86701 atggtcatct gctacacgca ggcagcagta gcctcgtgga aataggtcac acacaggtag
    86761 acccatatgg taaacgctgt tattgcggga accacggctg cctcgaaacc atcgccagtg
    86821 tggacagtat tcttgagctg gcacagctgc gtctcaatca atccatgagc tcgatgttac
    86881 atgggcaacc gttaaccgtg gactcattgt gtcaggcggc attgcgcggc gatctgctgg
    86941 caaaagacat cattaccggg gtgggcgcgc atgtagggcg cattcttgcc atcatggtga
    87001 atttatttaa cccacaaaaa atactgattg gctcaccgtt aagtaaagcg gcagatatcc
    87061 tcttcccggt catctcagat agcatccgtc agcaggccct tcctgcgtat agtcagcaca
    87121 ttagcgttga gagtactcag ttttctaacc agggcacgat ggcaggggct gcgctggtaa
    87181 aagacgcgat gtataacggt tctttgttga ttcgtctgtt gcagggttaa cattttttaa
    87241 ctgttctacc aaaatttgcg ctatctcaat ttgggccagg aaagcataac ttagactttc
    87301 aaggttaatt attttcctgg tttatatttg tgaagcataa cggtggagtt agtgatgctg
    87361 aagcgtttct ttattaccgg tacagacact tctgtaggga aaacggtggt ttcccgcgca
    87421 ttgctacaag cgttagcctc ccagggaaaa acggttgcgg gatataaacc cgtagcgaag
    87481 gggagcaaag agacacccga agggctgcgt aataaagatg ccctggtgtt gcagagtgtt
    87541 tcaaccatcg aactgcctta tgaagcagtt aatcctatcg cgttaagcga agaagaaagt
    87601 agcgtggcgc acagttgccc aatcaattac accctcattt caaacggcct ggcaaacctg
    87661 accgaaaaag tcgatcatgt cgtggtagaa gggactggcg gctggcgtag tctgatgaat
    87721 gatttgcgtc cactctctga atgggtggtg caggaacaac tgccggtgtt gatggttgtc
    87781 ggtattcagg aaggttgcat taaccatgca ctgctaacag ctcaggcgat tgccaacgac
    87841 gggctgccgc tcattggctg ggtggctaac cgaatcaacc caggactggc gcattatgcg
    87901 gaaatcattg atgtgctcgg taaaaaactt ccggcaccgc tcattggtga actgccttat
    87961 ctgccgcgcg ccgaacagcg tgaactgggg caatacatcc gcttagctat gctgcgcagt
    88021 gtgctggcgg tagatagagt cacggtgtaa cgtccgcgag attaccgacg caattacgca
    88081 ggcaatcaat aaaccgggga gtagctgata ctccccggtc atttcacata tcatcaacgt
    88141 cgacataatc ggcgcgtgcg tggttgccgc caacagtgtc gccatcccgg tcaatccgag
    88201 taaaagcgta atttcttcgc catcagggaa ccataatccc agactacgac catacaacat
    88261 gccaatggca agaccgataa atagcgtcgg tgtaaagaca ccaccgggtg cgccggaacc
    88321 gctactcgcc agcacggcac acagtttaca aaggaagatc ccggcaatga tcattaacag
    88381 tggcggggcg gttaaaaagg attgtacggt gctatagccg ttgccccaca ctgcaggtgt
    88441 gaacagggaa agcagaccca cgatcaaccc gcctagtgcc agttgccagg gcggcgcaag
    88501 tttgagactc acaaatccac gatgacaggc gttcattaac gttaacaaca gtggtccgca
    88561 cagacctgcc agcacacctg tactgataat cagcgcatag tcacgagcct gaaccgtcac
    88621 tgagagttgt acgctgtaga gtaacgcgtc gctatgatta atcagattgc taaccagcaa
    88681 tgccacgacg gcggaaataa tcaccgggcc gagagaggcc aacatcatag tgccaaacag
    88741 cacttcggct ataaataaac tgccagcaag cggggcacga taggccgcag ccattcccgc
    88801 cgcggcccca caggcgatcc ataatttcca ctcctggcgt ggcgtaaaac gttgggcaaa
    88861 acaggaggcg gcaagggcag ctaaaagaat catcgcacct tcgcgaccaa ttgcactgcc
    88921 gctggttact accagcagag aggcaagcga tttaaccagg cttgctgcgt aatcaaactg
    88981 accatcggtt tgcaacgctt ccatgtaatc ggtcggcgca tgagggcgtt gctgggtaaa
    89041 tttctgccag cccatcaaca acaaacccgc cgccagtccg ccgagcgccg gagttagcag
    89101 ccgtcgccag ggggaaaggt ttgtcgctgc attgaccaga ctgccggagt cattattgag
    89161 gaacaaccac tccagtagca gcatcgcatg acgaaacccg gcaacggcaa aggccgcgag
    89221 aataccgacg actgttgcga taagcagacg gcggaacata gtgcgcagat ccggataggt
    89281 gtgtagatga tgcatgagca gaaatgggca atgaaacacg ggcgactatt ttgcggcaaa
    89341 agaggccagc tgcaaaagca gtgcgtgctt taagagacaa aaaacgtgac actgtaacgt
    89401 attgttgccg aatcgcgcgt aatcgtcacc atccggcaat attacggtga tcctatcgaa
    89461 agagtggttt aaccgcgaca ggaattaaca gttgtgattg ccattgcgcc agcgttaagc
    89521 gagccagttc acccagtgca cggtagaaag ggtgttctgc tttttcgata aaaacgtcaa
    89581 gaaaacgtgt tgaccaggga aaaaggtgcc atgccagcaa ttcttcgcat tctgtctggc
    89641 gaccattctc cgccaaccac gcagccatca acagcagtga accaaaatga tcttccggtt
    89701 cgttttgctt catttcaaac tgaatgcctt tctcgcgcat ccactgacga agtgccaatg
    89761 ttgaatcgcc aaacagcaca gattcgcgat ccagccagac cgacccccac ggcggagacg
    89821 gcagtgccca cgggccgaca aacaaacgct gccaggcctg ggcgtgagtc tcttcacact
    89881 gtgtctgaaa agccgtcacc agcggcgcta atgacgcttc tggtagaggc cactgagttt
    89941 cccagccgtc actggtgagt accgcaacca gaggtgcggc ttcagcactc tctggagcgt
    90001 aataaaacag cgcgcccaac acgcgcgccg cgacagaaaa attatcttgc tgtgaaaaat
    90061 gggtcattcc taacatcctg aaatgtgcgg gttgccccgc accaatttgt taacctgcaa
    90121 tagccattcc taccgtcatg tgcaggccat aaaagagcac acgcccgatc atctcaccgc
    90181 caagaatgag gattaacccc agaataagtc cggcaacgtg aggttcacgg cgacgtatga
    90241 gcggacaaag ccagcatcct agacctgcgc ataacaatac cacacgccag acctgtaagg
    90301 aggcgtaatc tggtaccagt gcgctggctt gttgcacgga tgagtgaata gacgacagag
    90361 acaaaccttg cagcacaatc accccggcgc aagcgatcag cgccagaacg ctaatgatgg
    90421 caaatggtgt ggtattaaat gtaacgcggg ctgcccgcag aattgcggca gcaagtattg
    90481 gaccactcaa caataccgtc acgaagaaag ccagcgtagt gtaaccgtta tgccaggttg
    90541 gcacagtgtc gatttgatac acgcaggtca tcatccagac gaaaatgacg ccgagcgcca
    90601 tgctgacaag taaccagagt ttccccaacg cttgcggcat tttaccgatg accgccacca
    90661 gccaccatag tccgccaacg gcaaaaaata tggaaccggc agcaatctcg ttactcagac
    90721 cagaagctcc gattcgattc agcgagttaa acgcccgcag tggcgatccc aggtgcatga
    90781 cagaggcaat aaacccgatg cccatcaaca gccagaggaa aaacatgcca cgaacaatgt
    90841 gctgacggtc tgcgtcagtt ttggcggcga accagccaat tccgctaaca atcagcgcgc
    90901 ctactacgca ttgacccaat acggtaaaga taaccagtgg ccattcatgc catccatttc
    90961 ccattttaca cttcctccgg attagccaga taaccagtgg tatcgccggt cgggcggctg
    91021 ttggcgttag gtttgataac gatattgggt tttgtgaaat gcgcgcgcgg cagtggcgcg
    91081 acggcagcaa gagtgccgtg cttctggcgc agctcttcaa ttggaccgaa ctcaagtgca
    91141 cgcagcgggc aggattcgac acatatgggt tgtttcccct cggcgacgcg cgaataacaa
    91201 ccatcgcact tcgtcatgtg ccctttttca gcattgtact gtggcgcgcc gtacggacaa
    91261 gccatgtggc agtagcggca gccgatacaa acatcttcat caaccaccac aaagccatct
    91321 tcacgcttat gcattgcgcc gctggggcag acttttgtac atgcggggtc atcgcaatgg
    91381 ttacaggaga tggagagata ataagcgaac acgttctggt gccagacgcc attatcttcc
    91441 tgccagtcgc cgccagcata ttcataaata cggcggaaac tgacttccgg gccaagatct
    91501 ttaaaatctt tgcacgccag ttcgcaggtt ttacagccag tacagcggct ggaatcgata
    91561 aaaaatccat attgtgtggt catgggctac tccttaaacc ttttcgatct ggacaagatt
    91621 gctgtgcgac gggtttccct ttgccagcgg tgaagggcgg tgagaggtca gaatattgat
    91681 actgccgccg tgatcgaccc ggtcaccaaa catatccgct ttaagccacg caccttgccc
    91741 gatggcggta acgccaggca gaatacgcgg agtcactttt gcggcaatca gcatttctcc
    91801 attattgtta aatacccgca cggtatcgcc atgacggata ccgcgagcct gagcatcaat
    91861 ggggttgatc cacacctctt gtgggcaggc ctgctgtaac acatcaatat tgccgtagct
    91921 ggagtgggta cgcgctttgt agtggaagcc cgttaactgc agtggatagg ttttccgcag
    91981 gggatcgtcc cagccatcaa aacctggggt atacgcagga aggggatgaa taatttcatc
    92041 ttttttcaat tcccaggtat ctgcaatctt cgccagtcgt tcagaataaa tttcgatttt
    92101 ccccgaaggt gttttcaacg ggtttgcctg tggatcttca cggaatgcgc ggaaagcgac
    92161 gtagtgttct tccgggcatt ttttcttaaa gatcccggtc gttttcatct cctcgtagtc
    92221 gggcatctca gggttacgtt ccttcgtttt cgcatggaga tatttgatcc attcatgctg
    92281 actgcgacct tcagtaaagg tttgataaac gtccggtcct aagcgtttgg cgacttcact
    92341 cagcatccag tagatgggtt tgcgttcaaa ttttgctgag gttgcgggtt gggcgaggat
    92401 cacatagccc atattccctg cagattcatg agagataagg tcttcttgct ctgttggcat
    92461 caggtcgggc aacaggatat cgcaatactt agccgaggcc gtcatgaagt ggtcaatgcc
    92521 aacaatcatc tcgcacttgc tgtcatcctg aagcacctca ttggtgtgat tgatgtcgcc
    92581 atgttgattg atcaatgtgt tactggcgta gcaccataaa aacttgatgg ggacatccag
    92641 tttttctttt ccacgaacac catcacgggt cgcggtcatt tccgtaccat ggtcgatggc
    92701 atctgtccat gtaaagacgg aaatctgcgt tttaacagga ttctcgagca tcgggaacca
    92761 ttctaccccc agatcccagc taccttcgcg tacacctgag ttgccgccgt ttatgccgac
    92821 gttaccggtg agaacggaaa gcatggcaat agcgcgggat gtttgctcgc cgttggaatg
    92881 tcgttgtggc ccccaaccct gacaaatata agcaggtttt gctgaaccga tctctcgtgc
    92941 caactggata attttttctg ccgggatgct ggtgattttt gctgcccatt ccggcgtttt
    93001 agctatgccg tcaggccctt cgcccagaat ataggcttta taatgcgcgt tacgtggtgc
    93061 gttggcgggc agcgtttttt catcgtaacc aacacaatat ttgtcgagaa atggctgatc
    93121 gaccatgttt tcactaatca gtacccaggc aatcgcacag gccagtgcgc catcggtgcc
    93181 agggcgaatg ggcagccatt catcttcacg cccggcagct gtgtcgttat aacgtggatc
    93241 gatgacgatc atgcgtgcgt ttgaacgttc gcgggcttgc tcgacgtagt aagtgacacc
    93301 accaccgctc atccgcgttt ctgccgggtt atttccgaac ataacgacca gtttcgtatt
    93361 ggcgatatca tccgggctgt tgccatcatt ggcaccgaac atataactca ttgcggcact
    93421 gatctgtgcg gtactgtagc tgccatagcg actgagaaaa ccaccgcaag agttcatcag
    93481 acggtacggg acgtttgagt tggtgatgtt tccgccatct acgcctgttc cgtacaggac
    93541 atgtacagcc tcattgccgt aatctttcag gatccgccga agattatcac tgatggtatc
    93601 cagggcttcg tcccaactta tccgttcaaa tttaccttca ccgcgcttgc cgacgcgctt
    93661 catgggatat ttcaacctat caggatgatt catccgtcgg cggatagagc gcccgcgtaa
    93721 acacgcacga acctgatgat taccgtagac gtcgtcacct gtcgtatcag actccaccca
    93781 gtacacggtg tcatctttca catgcaaacg taacagacag cggctcccgc agttaacggt
    93841 gcaggaactc cagaccgctt tctcttctac cggtgcctct gccgctcgga ccatttggga
    93901 aaatggcaga gtgaaagcac tgcttgccag cgcaagactg ccaagtgcgg aggttttcat
    93961 cagacttcta cggctgattt cagccttcat gagcgcctct gtggtatgga ttttcatcat
    94021 tactcactta ttgcttttca aacaaaatgt catgccagaa tttatggttg tagtgggtta
    94081 tattttttcg atctcgacca gattcgtgtg ctgcgggttt cccttcgcca gtggtgaagg
    94141 gcgcagagtg gttagcgtat tcacacatcc gccatggtcg attttatcgc cagacatatt
    94201 ggcctcgtgc caggctccct ggcccatagc gctaacccca gggagaatgc gtggtgtcac
    94261 tttggctggt agccgaactt cgccacgatg gttatacacc cgcaccatat cgccgttggc
    94321 aatcccacgt ttctgtgcgt ctatagggtt gatccacacc tcctgacggc aggcagcctt
    94381 caggacatca atgttgccgt aggtcgagtg agtacgggat ttgtaatgga aaccaaacag
    94441 ttgcagtgga aaggtactac gttcagggga gtcccagcct tcaaaggttg aggcataaac
    94501 tggcagtggg cttatcactt catctttttc cagttcccag gtacgggcaa tttccgccag
    94561 cttgctggaa taaatttcaa tcttaccgga aggtgtttta agtggatttg cctcggggtc
    94621 gtcacgaaat gctttgtagg cgacaaaatg accattggga tctttacgct tatagatacc
    94681 catttttttc agttcgtcgt aagacggtaa cgccggatct ttggcaagca ttttggcgta
    94741 cagatgttgt aaccattgtt cctgcgtgcg accttcagtg aacttttgat agacgtcagg
    94801 tccaagacgt ttcgcgactt cactcaggat ccagtaaatc ggtttgcgtt cgaatttttc
    94861 gctggtgaca ggctggagga aaatgagata tcccatgtta ccggcgtagt cgttaggaat
    94921 aatatcttcc tgctcaacgg tcatcaggtc tggcagcaga atgtcggcat attttgccga
    94981 tgaggtcata aagttttcga tgaccacaat catttcgcat ttcgattcgt cctgcagaat
    95041 ttcatgcgtt ttgttgatgt cagaatgctg attaaccagg gtatttcccg cgtagttcca
    95101 gatgaactta atgggcacat ccagtttatc tttgccccgg acaccgtcgc ggattgccgt
    95161 catttgtggg ccatgatcga tagcatctgt ccagctgaag caggaaattg acgttttgac
    95221 cggattatcc agcaccggca ggcgctctat ggtaatggta taggtcgatt cacgcgcgcc
    95281 actatttccg ccgctgatgc cgacattgcc cgtcagaata ggcaacatag caatagcgcg
    95341 tgcggtcagc tcgccgtttg cctggcgttg cggcccccag ccctggcaga tataagcggg
    95401 ttttgctgtg ccaatttcac gcgccagttt gatgatacgg tccaccggga taccggtaat
    95461 ttgcgaagcc cactgcggcg ttttcgctgt tttatcgtca ccttcaccaa gaatataggc
    95521 tttatagtga ccatttttgg gtgcatctgc gggtaaggtt ttttcgtcat agccgacgca
    95581 gtatttatcg agaaaaggtt gatcaacgag attttcgtta atcaataccc aggcaatacc
    95641 cgcaaccagc gcggcatcgg tgcccgggcg aataggaagc cattcatctt cacgaccagc
    95701 agccgtatcg gtatatcgcg gatcgataac aatcattttt gcgttcgatt tttcgcggga
    95761 tttttcaaga agataagtga tgccaccgcc gctcatgcgg gtttctgccg ggttgttacc
    95821 aaacatcacg accagcttgc tgttttcaat atccgtggtg ctgttgccat cattactgcc
    95881 gtaggtgtag ggcatggcac aggaaatttg cgcagtgctg taggagccat actggttgag
    95941 tgaaccgccg tagcagttca tcaggcgttt gaccgccgag gctgatggcg aagagcgggt
    96001 tatattgccg ccaacgatcc ccgaagagta ctgaatatat acagcctcat tgccatattg
    96061 ttcgacggtt ttcttcaggc tactggcgat agtatccagg gcttcatccc agctaatccg
    96121 ttcgaatttg ccttcaccgc gtttacccac gcgtttcatt gggtaattca agcgatcggg
    96181 atgattaata cgccggcgga tggagcgacc gcgcaaacag gcgcgtacct gatggttgcc
    96241 atactcatcg ctgccggtgt tgtcagtttc cacccaggcc acttcattat ctttaacatg
    96301 tagacgaagt gcacagcggc taccacagtt gacggaacag gcaccccaga ccacttgttc
    96361 gctggcctgt tgtactgctg ccgctgcact gcgcagggtg aacggcaaag aaaaaccgcc
    96421 tgcagccagc gccagagaac ctatcgcggt agatttaacg agtgttctgc ggctgatgcc
    96481 caccattcgt tcatttttgg acataactca ctccctgttc tttatcgtta tataaaagtt
    96541 tatat
//
LOCUS       DAZKIP010000005        96515 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZKIP010000005 DAZKIP010000000
VERSION     DAZKIP010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 96515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 96515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..96515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.005"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(212..2071)
                     /gene="gss"
                     /locus_tag="ACHOUK_000388"
     CDS             complement(212..2071)
                     /gene="gss"
                     /locus_tag="ACHOUK_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFP9895908.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKE"
     gene            2276..3142
                     /gene="yghU"
                     /locus_tag="ACHOUK_000389"
     CDS             2276..3142
                     /gene="yghU"
                     /locus_tag="ACHOUK_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFP9895909.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3265..3513)
                     /gene="hybG"
                     /locus_tag="ACHOUK_000390"
     CDS             complement(3265..3513)
                     /gene="hybG"
                     /locus_tag="ACHOUK_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFP9895910.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3526..3867)
                     /gene="hypA"
                     /locus_tag="ACHOUK_000391"
     CDS             complement(3526..3867)
                     /gene="hypA"
                     /locus_tag="ACHOUK_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFP9895911.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEVHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3860..4348)
                     /gene="hybE"
                     /locus_tag="ACHOUK_000392"
     CDS             complement(3860..4348)
                     /gene="hybE"
                     /locus_tag="ACHOUK_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFP9895912.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHALSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4341..4835)
                     /gene="hybD"
                     /locus_tag="ACHOUK_000393"
     CDS             complement(4341..4835)
                     /gene="hybD"
                     /locus_tag="ACHOUK_000393"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFP9895913.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKSAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4835..6538)
                     /gene="hybC"
                     /locus_tag="ACHOUK_000394"
     CDS             complement(4835..6538)
                     /gene="hybC"
                     /locus_tag="ACHOUK_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFP9895914.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6535..7713)
                     /gene="hybB"
                     /locus_tag="ACHOUK_000395"
     CDS             complement(6535..7713)
                     /gene="hybB"
                     /locus_tag="ACHOUK_000395"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFP9895915.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7703..8689)
                     /gene="hybA"
                     /locus_tag="ACHOUK_000396"
     CDS             complement(7703..8689)
                     /gene="hybA"
                     /locus_tag="ACHOUK_000396"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFP9895916.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8692..9810)
                     /gene="hybO"
                     /locus_tag="ACHOUK_000397"
     CDS             complement(8692..9810)
                     /gene="hybO"
                     /locus_tag="ACHOUK_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFP9895917.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9999..10286)
                     /gene="yghW"
                     /locus_tag="ACHOUK_000398"
     CDS             complement(9999..10286)
                     /gene="yghW"
                     /locus_tag="ACHOUK_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFP9895918.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10405..11292)
                     /gene="yghX"
                     /locus_tag="ACHOUK_000399"
     CDS             complement(10405..11292)
                     /gene="yghX"
                     /locus_tag="ACHOUK_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFP9895919.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIFAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLNSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11449..12489
                     /gene="gpr"
                     /locus_tag="ACHOUK_000400"
     CDS             11449..12489
                     /gene="gpr"
                     /locus_tag="ACHOUK_000400"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFP9895920.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPEDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDERVTSVLVGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12529..13023)
                     /gene="yqhA"
                     /locus_tag="ACHOUK_000401"
     CDS             complement(12529..13023)
                     /gene="yqhA"
                     /locus_tag="ACHOUK_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFP9895921.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13214..14098
                     /gene="yghA"
                     /locus_tag="ACHOUK_000402"
     CDS             13214..14098
                     /gene="yghA"
                     /locus_tag="ACHOUK_000402"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFP9895922.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14370..14795)
                     /gene="exbD"
                     /locus_tag="ACHOUK_000403"
     CDS             complement(14370..14795)
                     /gene="exbD"
                     /locus_tag="ACHOUK_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFP9895923.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14802..15536)
                     /gene="exbB"
                     /locus_tag="ACHOUK_000404"
     CDS             complement(14802..15536)
                     /gene="exbB"
                     /locus_tag="ACHOUK_000404"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFP9895924.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15788..16975
                     /gene="metC"
                     /locus_tag="ACHOUK_000405"
     CDS             15788..16975
                     /gene="metC"
                     /locus_tag="ACHOUK_000405"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFP9895925.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            17115..17774
                     /gene="yghB"
                     /locus_tag="ACHOUK_000406"
     CDS             17115..17774
                     /gene="yghB"
                     /locus_tag="ACHOUK_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFP9895926.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17814..18713)
                     /gene="yqhC"
                     /locus_tag="ACHOUK_000407"
     CDS             complement(17814..18713)
                     /gene="yqhC"
                     /locus_tag="ACHOUK_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFP9895927.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMARPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18907..20070
                     /gene="yqhD"
                     /locus_tag="ACHOUK_000408"
     CDS             18907..20070
                     /gene="yqhD"
                     /locus_tag="ACHOUK_000408"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311921.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFP9895928.1"
                     /translation="MNNFNLHTPTRILFGKSAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLGVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            20175..21002
                     /gene="dkgA"
                     /locus_tag="ACHOUK_000409"
     CDS             20175..21002
                     /gene="dkgA"
                     /locus_tag="ACHOUK_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFP9895929.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     FDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHNRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            21202..22124
                     /gene="yqhG"
                     /locus_tag="ACHOUK_000410"
                     /pseudo
     CDS             21202..22124
                     /gene="yqhG"
                     /locus_tag="ACHOUK_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
     gene            22173..22430
                     /gene="yqhH"
                     /locus_tag="ACHOUK_000411"
     CDS             22173..22430
                     /gene="yqhH"
                     /locus_tag="ACHOUK_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFP9895930.1"
                     /translation="MKTIFTVGAVVLATCVLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22473..24692)
                     /gene="ygiQ"
                     /locus_tag="ACHOUK_000412"
     CDS             complement(22473..24692)
                     /gene="ygiQ"
                     /locus_tag="ACHOUK_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFP9895931.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQCQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24803..26215)
                     /gene="ftsP"
                     /locus_tag="ACHOUK_000413"
     CDS             complement(24803..26215)
                     /gene="ftsP"
                     /locus_tag="ACHOUK_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFP9895932.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26290..27027)
                     /gene="plsC"
                     /locus_tag="ACHOUK_000414"
                     /gene_synonym="parF"
     CDS             complement(26290..27027)
                     /gene="plsC"
                     /locus_tag="ACHOUK_000414"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFP9895933.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(27261..29519)
                     /gene="parC"
                     /locus_tag="ACHOUK_000415"
     CDS             complement(27261..29519)
                     /gene="parC"
                     /locus_tag="ACHOUK_000415"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFP9895934.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(29657..31264)
                     /gene="ygiS"
                     /locus_tag="ACHOUK_000416"
     CDS             complement(29657..31264)
                     /gene="ygiS"
                     /locus_tag="ACHOUK_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9895935.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPTNTQLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGRRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAT
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDQWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGEIRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKALLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQAMQITDATKRNALYQQAEVIVNQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(31373..31855)
                     /gene="ygiV"
                     /locus_tag="ACHOUK_000417"
     CDS             complement(31373..31855)
                     /gene="ygiV"
                     /locus_tag="ACHOUK_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFP9895936.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCRPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETNVYVPLA
                     "
     gene            complement(31908..32300)
                     /gene="ygiW"
                     /locus_tag="ACHOUK_000418"
     CDS             complement(31908..32300)
                     /gene="ygiW"
                     /locus_tag="ACHOUK_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFP9895937.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            32452..33111
                     /gene="qseB"
                     /locus_tag="ACHOUK_000419"
     CDS             32452..33111
                     /gene="qseB"
                     /locus_tag="ACHOUK_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFP9895938.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            33108..34457
                     /gene="qseC"
                     /locus_tag="ACHOUK_000420"
     CDS             33108..34457
                     /gene="qseC"
                     /locus_tag="ACHOUK_000420"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFP9895939.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPVMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNVQGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            34567..35148
                     /gene="mdaB"
                     /locus_tag="ACHOUK_000421"
     CDS             34567..35148
                     /gene="mdaB"
                     /locus_tag="ACHOUK_000421"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFP9895940.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            35179..35493
                     /gene="ygiN"
                     /locus_tag="ACHOUK_000422"
     CDS             35179..35493
                     /gene="ygiN"
                     /locus_tag="ACHOUK_000422"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFP9895941.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(35538..36425)
                     /locus_tag="ACHOUK_000423"
     CDS             complement(35538..36425)
                     /locus_tag="ACHOUK_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9895942.1"
                     /translation="MIQKDKTRVDIYGERFRTRASQLTPGLRAVASYINEHREVVLDQ
                     TAMEIAATLNTSDATVIRAIQALGFAGLRDLKRTLEQWLGPALSSSEKMSTTVSNLTS
                     DVNTAIDFVLEGHLYTCNVLSEPENRHALAQAVALLVQARQVAIFGIGASGILADYTA
                     RLFNRIGLPATALNRTGIGLAEQLIALQRGDVLIMMAQKSAHREGLTTLREARRLGIP
                     VILLTNALDSRFSKDASIVIHVPRGDEKGKTPLHGTVLLCLEMIVWSVASAVPQRAVK
                     TIKRINDFHRGLKTGRKNG"
     gene            complement(36422..37369)
                     /locus_tag="ACHOUK_000424"
     CDS             complement(36422..37369)
                     /locus_tag="ACHOUK_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP9895943.1"
                     /translation="MRLFFSLLILLSFFARATEPVQVFTDDLGRKVTVPAHPKRIVSL
                     HDLDITIPLIELGVPPVASHGRTRPDGSHFIRSGALLTGVDFDNSSIAFIGTADIDIE
                     AIVAAKPDLIITEPTRNTPIERLEKIAPTVSIDHLKGGAPEIYRKLAELTGTQSQLAI
                     LERRYQAQINALKATLDSQKITVSVIQANQGKINVMHSYHSLGRVLRDAGFRFPPLIE
                     SIPEGGRMDVSAERLPELDADFVFATWRGDTGGKPQDELAAMEKVMPGWCQFLTACRS
                     GRYVLISREEAISNSFASLGLMAAQIQSQIAGRPLPEAK"
     gene            complement(37380..38417)
                     /locus_tag="ACHOUK_000425"
     CDS             complement(37380..38417)
                     /locus_tag="ACHOUK_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFP9895944.1"
                     /translation="MNVGLRPLRVGKFSTLVRPKNLVLLGGLFLFAVGILIFGLMHGS
                     FFVPASEVGRALFAPENVSTDARYIVQDIRLPRVIMALLCGAMLGMAGAAMQSIARNG
                     LADPGLIGVKEGCSVAVLWLIFQFPMLGMFWRPVAGLAGGLLVALIVIFCAREISRPR
                     FVLIGIGVSWFFAAGIGVFMTTADVRDVQTALMWLSGSLHAANWMLVGISACWMLPAA
                     LLLLFTARTADIALLGHQVATGLGVNSSRLALLRVAAPIILTAVCVSCVGNIGFVGLI
                     APHISRFILRGGQTTLLLGSAVSGALLVILADSIGRLAFLPLQLPAGIIISLIGGPFF
                     LLLLWQRRNSF"
     gene            complement(38426..39409)
                     /locus_tag="ACHOUK_000426"
     CDS             complement(38426..39409)
                     /locus_tag="ACHOUK_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFP9895945.1"
                     /translation="MMRTGLVTVIFLALLLVGCVVYPGIGARFIAPQTVLQAFLHFEP
                     QNFDHNVIVRLRLPRLAAALLTGASLGVAGALLQAVIRNPLGEPHILGLNAGAALAVV
                     AASALGLAFPVGRPLLASTGGALLFLLILLLSSAGRSGLTPMKVTLCGVALSAFVSSI
                     TAAILILDEQTLLAMRTWLAGDLAGQDWATLGTSAWFSLGGVVLAIYLAPSLNMLALG
                     DRMAQGLGVSVLRTRTFTLLAIALLCGAAVSIAGPIGFVGLLVPQIVRRLVSADLRVL
                     LPLSACVGALLLLLADIIARTLFTPHELATGVMTALVGAPVFVIMATRMFK"
     gene            complement(39406..40215)
                     /locus_tag="ACHOUK_000427"
     CDS             complement(39406..40215)
                     /locus_tag="ACHOUK_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9895946.1"
                     /translation="MAHNAKGQGLILDNVSAGYHKKIIVDGVSFSVPTEKMTVLVGAN
                     GCGKSTLLSTIARVLQPMGGSILLDGKAIHEQPTKALSRRLGILPQSPLLPEGLTVYE
                     LVSRGRFPWQNFIRQWSDADEQAVEEALKLTGTQEFAHLPVEKLSGGQRQRCWIAMVL
                     AQKTPYILLDEPTTWLDLRYQVEILELLHDLTRHHGRTVVVVLHDLNFAVNYGDTLIF
                     LRQGKVVRVLNEGEHCTPELVKAVFDVDVHASINTLTGKPFFMPFRGVEKV"
     gene            40589..42730
                     /locus_tag="ACHOUK_000428"
     CDS             40589..42730
                     /locus_tag="ACHOUK_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HFP9895947.1"
                     /translation="MAKFTPSFSGIKGRALFSLLFAAPMIHATDTATTKDGETITVTA
                     DANTATEATDGYQPLSTSTATLTDMPMLDIPQVVNTVSDQVLENQNATTLDEALYNVS
                     NVVQTNTLGGTQDAFVRRGFGANRDGSIMTNGLRTVLPRSFNAATERVEVLKGPASTL
                     YGILDPGGLINVVTKRPEKTFHGSVSATSSSFGGGTGQLDITGPIEGTQLAYRLTGEV
                     QDEDYWRNFGKERSTFIAPSLTWFGDNATVTMLYSHRDYKTPFDRGTIFDLTTKQPVN
                     VDRKIRFDEPFNITDGQSDLAQLNAEYHLNSQWTARFDYSYSQDKYSDNQARVTAYDA
                     TTGTLTRRVDATQGSTQRMHSTRADLQGNVDIAGFYNEILGGVSYEYYDLLRTDMIRC
                     KNAKDFNIYNPVYGNTSKCTTVSASDSDQTIKQESYSAYAQDALYLTDNWIAVAGIRY
                     QYYTQYAGKGRPFNVNTDSRDEQWTPKLGLVYKLTPSVSLFANYSQTFMPQSSIASYI
                     GDLPPESSNAYEVGAKFELFDGITADIALFDIHKRNVLYTESIGDETIAKTAGRVRSR
                     GVEVDLAGALTENINIIASYGYTDAKVLEDPDYAGKPLPNVPRHTGSLFLTYDIHNMP
                     GNNTLTFGGGGHGVSRRSATNGADYYLPGYFVADAFAAYKMKLQYPVTLQLNVKNLFD
                     KTYYTSSIATNNLGNQIGDPREVQFTVKMEF"
     gene            complement(42794..44686)
                     /gene="parE"
                     /locus_tag="ACHOUK_000429"
     CDS             complement(42794..44686)
                     /gene="parE"
                     /locus_tag="ACHOUK_000429"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFP9895948.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGNCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(44715..45296)
                     /gene="yqiA"
                     /locus_tag="ACHOUK_000430"
     CDS             complement(44715..45296)
                     /gene="yqiA"
                     /locus_tag="ACHOUK_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFP9895949.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(45296..46123)
                     /gene="cpdA"
                     /locus_tag="ACHOUK_000431"
     CDS             complement(45296..46123)
                     /gene="cpdA"
                     /locus_tag="ACHOUK_000431"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFP9895950.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(46148..46570)
                     /gene="yqiB"
                     /locus_tag="ACHOUK_000432"
     CDS             complement(46148..46570)
                     /gene="yqiB"
                     /locus_tag="ACHOUK_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFP9895951.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(46571..47200)
                     /gene="nudF"
                     /locus_tag="ACHOUK_000433"
     CDS             complement(46571..47200)
                     /gene="nudF"
                     /locus_tag="ACHOUK_000433"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFP9895952.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            47405..48886
                     /gene="tolC"
                     /locus_tag="ACHOUK_000434"
     CDS             47405..48886
                     /gene="tolC"
                     /locus_tag="ACHOUK_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFP9895953.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTASS
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            49034..49705
                     /gene="ygiB"
                     /locus_tag="ACHOUK_000435"
     CDS             49034..49705
                     /gene="ygiB"
                     /locus_tag="ACHOUK_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFP9895954.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            49711..50871
                     /gene="ygiC"
                     /locus_tag="ACHOUK_000436"
     CDS             49711..50871
                     /gene="ygiC"
                     /locus_tag="ACHOUK_000436"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFP9895955.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(50909..51697)
                     /gene="ygiD"
                     /locus_tag="ACHOUK_000437"
     CDS             complement(50909..51697)
                     /gene="ygiD"
                     /locus_tag="ACHOUK_000437"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFP9895956.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQTLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            51840..52613
                     /gene="zupT"
                     /locus_tag="ACHOUK_000438"
     CDS             51840..52613
                     /gene="zupT"
                     /locus_tag="ACHOUK_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFP9895957.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(52671..52841)
                     /gene="yqiD"
                     /locus_tag="ACHOUK_000439"
     CDS             complement(52671..52841)
                     /gene="yqiD"
                     /locus_tag="ACHOUK_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFP9895958.1"
                     /translation="MFIAWYWIVLIVLVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(53103..53756)
                     /gene="ribB"
                     /locus_tag="ACHOUK_000440"
     CDS             complement(53103..53756)
                     /gene="ribB"
                     /locus_tag="ACHOUK_000440"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFP9895959.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(53860..54008)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            54130..54429
                     /gene="ubiK"
                     /locus_tag="ACHOUK_000441"
     CDS             54130..54429
                     /gene="ubiK"
                     /locus_tag="ACHOUK_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFP9895960.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRSSELEARNNSVADLQSPPAIPPIDKAE"
     gene            complement(54464..54664)
                     /gene="glgS"
                     /locus_tag="ACHOUK_000442"
     CDS             complement(54464..54664)
                     /gene="glgS"
                     /locus_tag="ACHOUK_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFP9895961.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQTRELELEH"
     gene            54933..55547
                     /gene="yqiJ"
                     /locus_tag="ACHOUK_000443"
     CDS             54933..55547
                     /gene="yqiJ"
                     /locus_tag="ACHOUK_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFP9895962.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHTCIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDHFGQIHYLLLEPEEGKIFTKGDKVLIICRLSATRYLAENNP"
     gene            55589..57250
                     /gene="yqiK"
                     /locus_tag="ACHOUK_000444"
     CDS             55589..57250
                     /gene="yqiK"
                     /locus_tag="ACHOUK_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFP9895963.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNAKIAAFEA
                     ERRREAEQTRILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGGTAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSLLTPTTPVAENVE"
     gene            57489..57548
                     /locus_tag="ACHOUK_000445"
     CDS             57489..57548
                     /locus_tag="ACHOUK_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFP9895964.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            57865..57924
                     /gene="ibsE"
                     /locus_tag="ACHOUK_000446"
     CDS             57865..57924
                     /gene="ibsE"
                     /locus_tag="ACHOUK_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFP9895965.1"
                     /translation="MMKLVIILVVLLLLSFPTY"
     gene            complement(58044..59477)
                     /gene="hldE"
                     /locus_tag="ACHOUK_000447"
     CDS             complement(58044..59477)
                     /gene="hldE"
                     /locus_tag="ACHOUK_000447"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFP9895966.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPSSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVVA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(59525..62365)
                     /gene="glnE"
                     /locus_tag="ACHOUK_000448"
     CDS             complement(59525..62365)
                     /gene="glnE"
                     /locus_tag="ACHOUK_000448"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFP9895967.1"
                     /translation="MKPLSSPLQQYWQTIVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SISAHPEWLTELESQPPQADEWQHYVAWLQEALINVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLLRITALLAGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSTEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVPEDCFTAERELVRASWQKWLVEE"
     gene            complement(62388..63689)
                     /gene="ygiF"
                     /locus_tag="ACHOUK_000449"
     CDS             complement(62388..63689)
                     /gene="ygiF"
                     /locus_tag="ACHOUK_000449"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFP9895968.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLHHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            63931..64551
                     /gene="ygiM"
                     /locus_tag="ACHOUK_000450"
     CDS             63931..64551
                     /gene="ygiM"
                     /locus_tag="ACHOUK_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFP9895969.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            64615..65853
                     /gene="cca"
                     /locus_tag="ACHOUK_000451"
     CDS             64615..65853
                     /gene="cca"
                     /locus_tag="ACHOUK_000451"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFP9895970.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETENALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFTTLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPE"
     gene            complement(66034..66855)
                     /gene="bacA"
                     /locus_tag="ACHOUK_000452"
     CDS             complement(66034..66855)
                     /gene="bacA"
                     /locus_tag="ACHOUK_000452"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFP9895971.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTSGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(66946..67314)
                     /gene="folB"
                     /locus_tag="ACHOUK_000453"
     CDS             complement(66946..67314)
                     /gene="folB"
                     /locus_tag="ACHOUK_000453"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFP9895972.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DNVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            67420..68037
                     /gene="plsY"
                     /locus_tag="ACHOUK_000454"
     CDS             67420..68037
                     /gene="plsY"
                     /locus_tag="ACHOUK_000454"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFP9895973.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(68050..68982)
                     /gene="ttdR"
                     /locus_tag="ACHOUK_000455"
                     /gene_synonym="dan"
     CDS             complement(68050..68982)
                     /gene="ttdR"
                     /locus_tag="ACHOUK_000455"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFP9895974.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEIFTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVYFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            69189..70100
                     /gene="ttdA"
                     /locus_tag="ACHOUK_000456"
     CDS             69189..70100
                     /gene="ttdA"
                     /locus_tag="ACHOUK_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFP9895975.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATIKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            70097..70702
                     /gene="ttdB"
                     /locus_tag="ACHOUK_000457"
     CDS             70097..70702
                     /gene="ttdB"
                     /locus_tag="ACHOUK_000457"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFP9895976.1"
                     /translation="MKKILTTPIKAEDLQDIHVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPLPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            70751..72214
                     /gene="ttdT"
                     /locus_tag="ACHOUK_000458"
     CDS             70751..72214
                     /gene="ttdT"
                     /locus_tag="ACHOUK_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFP9895977.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(72257..73270)
                     /gene="tsaD"
                     /locus_tag="ACHOUK_000459"
     CDS             complement(72257..73270)
                     /gene="tsaD"
                     /locus_tag="ACHOUK_000459"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFP9895978.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            73508..73723
                     /gene="rpsU"
                     /locus_tag="ACHOUK_000460"
     CDS             73508..73723
                     /gene="rpsU"
                     /locus_tag="ACHOUK_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFP9895979.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            73834..75579
                     /gene="dnaG"
                     /locus_tag="ACHOUK_000461"
     CDS             73834..75579
                     /gene="dnaG"
                     /locus_tag="ACHOUK_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFP9895980.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            75774..77615
                     /gene="rpoD"
                     /locus_tag="ACHOUK_000462"
     CDS             75774..77615
                     /gene="rpoD"
                     /locus_tag="ACHOUK_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFP9895981.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKSRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(77694..78200)
                     /gene="mug"
                     /locus_tag="ACHOUK_000463"
     CDS             complement(77694..78200)
                     /gene="mug"
                     /locus_tag="ACHOUK_000463"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFP9895982.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDHQLKPQEAQHLLDYRCGVTKLVDRPTVQANEISKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLSIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            78325..78400
                     /locus_tag="ACHOUK_000464"
     tRNA            78325..78400
                     /locus_tag="ACHOUK_000464"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:78358..78360,aa:Ile,seq:cat)
     gene            complement(78454..79218)
                     /gene="nfeF"
                     /locus_tag="ACHOUK_000465"
                     /gene_synonym="yqjH"
     CDS             complement(78454..79218)
                     /gene="nfeF"
                     /locus_tag="ACHOUK_000465"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFP9895983.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            79506..80129
                     /gene="nfeR"
                     /locus_tag="ACHOUK_000466"
                     /gene_synonym="yqjI"
     CDS             79506..80129
                     /gene="nfeR"
                     /locus_tag="ACHOUK_000466"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFP9895984.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLNLRVNQSDITDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(80283..81803)
                     /gene="aer"
                     /locus_tag="ACHOUK_000467"
     CDS             complement(80283..81803)
                     /gene="aer"
                     /locus_tag="ACHOUK_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFP9895985.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTGKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYILCALVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            82221..83600
                     /gene="ygjG"
                     /locus_tag="ACHOUK_000468"
     CDS             82221..83600
                     /gene="ygjG"
                     /locus_tag="ACHOUK_000468"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFP9895986.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(83642..83974)
                     /gene="ygjH"
                     /locus_tag="ACHOUK_000469"
     CDS             complement(83642..83974)
                     /gene="ygjH"
                     /locus_tag="ACHOUK_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFP9895987.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRIV"
     gene            84193..85176
                     /gene="ebgR"
                     /locus_tag="ACHOUK_000470"
     CDS             84193..85176
                     /gene="ebgR"
                     /locus_tag="ACHOUK_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFP9895988.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            85360..88452
                     /gene="ebgA"
                     /locus_tag="ACHOUK_000471"
     CDS             85360..88452
                     /gene="ebgA"
                     /locus_tag="ACHOUK_000471"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFP9895989.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKT
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAIFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            88449..88898
                     /gene="ebgC"
                     /locus_tag="ACHOUK_000472"
     CDS             88449..88898
                     /gene="ebgC"
                     /locus_tag="ACHOUK_000472"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFP9895990.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVDEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            88961..90394
                     /gene="ygjI"
                     /locus_tag="ACHOUK_000473"
     CDS             88961..90394
                     /gene="ygjI"
                     /locus_tag="ACHOUK_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9895991.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            90528..91598
                     /gene="ygjJ"
                     /locus_tag="ACHOUK_000474"
     CDS             90528..91598
                     /gene="ygjJ"
                     /locus_tag="ACHOUK_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFP9895992.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASNDPSDQIFLMQYFYW"
     gene            91615..93966
                     /gene="ygjK"
                     /locus_tag="ACHOUK_000475"
     CDS             91615..93966
                     /gene="ygjK"
                     /locus_tag="ACHOUK_000475"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFP9895993.1"
                     /translation="MKIKTILTPVTCALLISFSAHATNADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYNIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTSQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKTWVAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGNKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVASGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPATQ
                     YFYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHFYMLYNDFFRKQ"
     gene            94492..96510
                     /gene="fadH"
                     /locus_tag="ACHOUK_000476"
     CDS             94492..96510
                     /gene="fadH"
                     /locus_tag="ACHOUK_000476"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFP9895994.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYHNRMRFAVEVVSAVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAMQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRTPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIAGFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
BASE COUNT        23858 a        25912 c        24353 g        22392 t
ORIGIN      
        1 tttttaacga taaaaagagt gttatttctt gtgtatcaga caataagcat tcattaaaaa
       61 tctttagaaa taattagaac acaggcattc tgctggttaa tgtattcaca tatgaaatga
      121 ataaatattt gaacatataa atattgaata ttttgattaa tccccgtact gactattctt
      181 cataatcagt acgggattgc tacaacatgt attactcttt caccacaatt aacggttcaa
      241 tatcactctc ttttttaata accagtgatt catcaccgcg caaacacgtc ccaccgtagt
      301 tgccgccaac ggtgaaggta catacctgaa tgtatttacc gtccactttc ggcaaacacc
      361 acagttgctg atagatgttt ttctgctcgg caaatttacc actggttttg tccagcacct
      421 cttcatgatg gctgacgagg tcgatgttgc tgccacagcg accggcgatc ggtttcactg
      481 cgtaacctgt tttcaccaat tcatcattaa cagtgaaatc ggtatccagc aggtaacgat
      541 ggtgcgggaa cagcgaccag aggatcggca gaatcgcttt gttgccgggg atcaccgtcc
      601 acagcggctc aaagaccagc acttccgggc gcagcaatac gtcgataaga cgcacttcat
      661 tttgcggatg accggtacgg atcggcaccg cagcaaactc acggtcgcta acttcacgaa
      721 tctgatcaaa cgcggtttcc cacgcccagg ttttccacac gcagttaacc agtcgccctt
      781 ccccatcaat cagttgcccg gcagcatccc agcccagttc atccagcccg cgcaagatac
      841 gcgtttcaaa gcccgcctgg tgcagcgcct gctccataaa ctgcgcgtga tagttttcct
      901 cgatatcttt gtcctgcatg atatggacaa acggacgtgc acgactgtgt ttccaggcac
      961 cagccagttc gttaatcagc ccttccgccg gattgaagcc gttgccttta tagccctgct
     1021 ccgcccaacg ttcgaggatc aagcccgctt cggtatgaca ggaggcggag tcggcgttgt
     1081 actcgtaaac cttcagacca cgctcatcca tgcagaaatc catacgaccg gtgatcatat
     1141 ggtgacggcg acgctgccag gagagacgca aacgtggcca gaggattttc ggaatgtcga
     1201 acagcgccag caggttgtca tctttcagca ccttgtcggt tgcgtgaaga tacatcaggt
     1261 gcagctcgtt ggtggcttta atcagctcct gctcggcgct ctcggtaatg gtgtagtaat
     1321 gatacggatc ctgattgatc acctgaccgt tggcctgcac ataggcgttt tgcagcggat
     1381 ctttttcatc cagccattta ccgtcaaact ggcctttgtt ttccagacgc gctccgctga
     1441 ttttcagcag ctcgcctgca atttccggct gcggtaagct gtattcagta tcttccgtct
     1501 ggatcatcca gcccagaatg gtggtgtcat caaaagtgtc tttaagggta tagcagccgt
     1561 tttcgaccac catctccagt tcgcgcgtcc actgttgccc ttgcggcaac ggggaatgaa
     1621 tcacgttctg ttccgcaata cggactttgt tgccatgtaa ttgggtaatg atggcgacat
     1681 ggccagtgtc tttaaattca ccgcctttat cccagataag aagcgcaccc gcgaccggcg
     1741 cacgcggcga gccgttagga aatgcctgca atggcaggat gttgtcatta accacttcac
     1801 gcaggaagcg cagcgagaaa atctcccacg ccatacccac gtcagtaaag accacaccgt
     1861 agttcagaaa gagaaaacgg cgagcaaatt caacgcattg ccatttgtgg cccatatatt
     1921 cgtcgtcgat atagctacgg aatacggcgt catcttcgta ttcctgcgga tcgagagaac
     1981 tgtaatctga agagtagatt gctaccccac ctggggcgta gcccaataat gtcccgaacg
     2041 gggcatcctg gctggtcgtt cctttgctca tcactttacc tttaacaata caacctaagc
     2101 agttggcgta attgttgtgc tctgattacc tgctcagcga cattaaccgg acagaggtca
     2161 gactaatcat acacctcatc gcagcggctg gcgcaaaggg ttaaaaaatt cacattctgt
     2221 acagcaagtg acctgctaca ctgcttcaac actaccactc agaaggcaac tcactatgac
     2281 agacaatact tatcagcccg cgaaagtctg gacgtgggat aaatccgctg gcggcgcgtt
     2341 cgccaatatc aatcgcccgg tttctggtcc gacgcatgaa aaaacgctgc cggtgggcaa
     2401 acatccatta caactttatt cgctgggaac gccgaacggt cagaaagtaa cgattatgct
     2461 tgaggagctg ctcgcgctgg gcgttactgg cgcagagtac gatgcctggc tgatacgcat
     2521 aggcgatggt gatcaattct ccagcggctt tgtcgaagtg aacccaaatt cgaagatccc
     2581 ggcgctgcgc gatcataccc ataatccgcc gatccgcgtg tttgagtcag gatcgattct
     2641 gctttatctg gcggagaaat ttggctactt cctgccgcag gatctggcaa aacggactga
     2701 aacgatgaac tggctgttct ggttacaggg cgcggcaccg ttcctcggcg gtggttttgg
     2761 tcacttttac cattacgcgc cggtgaaaat tgagtacgcc atcaatcgct ttaccatgga
     2821 agccaagcgc ctgctcgacg tgctggataa gcaactggcg cagcataagt ttgttgcggg
     2881 cgatgagtac accattgcgg atatggcgat ttggccgtgg tttggcaacg tggtgctcgg
     2941 cggcgtgtat gatgccgcag agtttctgga tgcgggaagt tataaacatg tgcaacgctg
     3001 ggcgaaagaa gtgggcgaac gtccggcagt gaagcgtggg cgtattgtta accgcaccaa
     3061 cgggccgctg aacgagcagt tgcatgagcg ccatgacgcc agtgattttg agacgaatac
     3121 ggaagataag cgtcaggggt aaggattggt gttcgtcgca gcaagccatc caggccggat
     3181 aaggcgttca cgccgcatcc ggcaatcgag cacaatgcct gatgcgacgc tgccgcgtct
     3241 tatcagacct acgaaagcta atcatcacgc gctggtaatg tcgtaatcca tttggcgcag
     3301 tgcgtctaat gtggctttgg cttcatcttc gtcgatgatg ctcatggcaa atccgacgtg
     3361 taccagcacc cactggccca gcagatcggc agggttacct tcacaaatca gggcgatatt
     3421 cacgtcgcgc ttgataccac atacttcaac ctgcgcaagc tggtgaatat cttcaccgac
     3481 agccagcacc tggcctggaa cgccaataca cattgttaac tccggttatt caacttcaat
     3541 acttttgacg atcagcgaat cgccggtatc gacacgcaac cgctcgccgt gacagagcgg
     3601 acactgcgca tcgtgctgat gaacctccac cacctggctg caatcccagc accaggcctg
     3661 ggcgggttta tagacgatat gtaaatcgca cccttgcgcc accgttccgt ggcagacaat
     3721 ttcaaaacta aaacggacgg cgctctcctc aacgcaggag agcgcgccaa tttccagcca
     3781 cacggcggtg acgcgcttaa catcgtgctg ctccgcctgc cgctggataa tttcaacggc
     3841 gctctggcaa agcgacaact catgcatttt cgccactcct gcgaccaaac agcagggcgc
     3901 gacgccctgc gtgtggtaca tccggattcg tgactggcag cgaaaggatc attcgtgcgc
     3961 agtcatccgt caggcgttgg ccctcttcaa tcgacatact gtgcgaaagc ggtgacatca
     4021 gcgaacagga gaggtattgc gaaacaccgt ccagttcgcc aacggtaaag gtcatggtgc
     4081 catacggcag ttgcagacca attttttcac tcactttgcg cagcggccag agttgatccg
     4141 ggccggggaa gataactgca ctcagcatcc acggggtgat cacacacccc gtccactgac
     4201 cttcgaacag cgtaaaatca gaaacataca ccggcattga aggatgcaga aaagagagag
     4261 cgtgcatcga acgccgggca atttcttcaa acgctgcctg tacttgcgcc ttcggggagg
     4321 tctggaaacc tgctatctct tcagtcatga atcgcctccc gtgggatggc ttccacgcca
     4381 gattcacgca gcgcagccag aacctgctca agcgcaggtt caatcattgc ttcaaccgtc
     4441 ggcgttaagc cgatgtgtgg ctccagcgat tccggaatta cgccgaccag ggtcagcttt
     4501 ttcggaaact cgccggtgaa gcgcagggcc gacaagacgt cggccaggcc aagctgatgc
     4561 ggagagattt tgttggtaaa caacgccgga acttcttcat cccgcaggat catcatcgtt
     4621 cccggcgcgc tcttcttcga cacaatggca tccgcaataa tcagatgatc gcgatttgcc
     4681 atgtcgccaa gcagctccat tcccgccgtg ccgccatcga ggatctcaac ataatccggc
     4741 agaatgtatc gttgctctaa cgcttcgaca atccgcacac cgatggcttc atcggtcagc
     4801 aaaatattgc cgacccctaa gactaaaata cgcattacag aaccttcact gaaaccactt
     4861 cgttgccgtc agcatccact acgtgtaccg cacaggccat gcacgggtcg aaggagtgaa
     4921 tggtacgcac cacttccagc ggtttattcg gatcagcaac cggtgtaccc accagcgact
     4981 gctcgtaagg accgacgtca tcattgaagt tacgcgggcc agagttccag gttgatggaa
     5041 caactgcctg gtagttgctg atgataccgt ctttgatcac catccagtga gagagcatac
     5101 cgcgcggcgc ttcgaggaag ccaacacctt tgaactcacc cgttgccgga atgttcggct
     5161 tcacaaaggt ggtgtgatcg cctttgccga tattggtgat cagtgcactg tattggtttt
     5221 gcaggatatc ctgcaattca cagcagtgaa cggtacgacc aataatacgg cccagcgtgg
     5281 agtgcagctg tgccacttcc agcgtgttgc cagtcagttt ctgataaatc gcaacgattt
     5341 cattcagttt gttctgggta gattcgcgac ctgccgccag tttcaccagc atattagcca
     5401 gcggacccac ttctaccgtt ttgccgtaga aagtcggtga tttcacccag gaatatttcc
     5461 cgtcgtcaga ccaaccatca taagccggaa tggtggtacc ttcccacggt gcctgcggcg
     5521 cttcgtcttt ataccaggag tgcttcgcgc tttcctgaat gcctttgatc aggtattcat
     5581 cggaatgaga agtgatcgga cgatacgagg acagatccgc attctcaatg tagccgcccg
     5641 ggaacaggaa gctgccgttt ttactgtcgg tcgggaattc cggcacgctc aggtagttca
     5701 ccgcaccttt accgcgcgtc agccattccg ggtagaacgc agcgataacc gcagtatcga
     5761 ccttgtaaac ctgctcaaca aagtcactca gcttgtcgat gaaagacttg atgtacatca
     5821 ggcgctcaag gttcagcacg cccagaccgt cgaggttgat tgggttagcg accccaccta
     5881 ccgccaggtt ctgaatgtgc ggcgttttac cgcccagcag cgccacgacg cggttagcgt
     5941 cacgctggca ctccagcgcc tgcaggtagt gcgctaccgc aatcaggttc acttccggcg
     6001 gcagtttcat cgccgggtga ccccagtagc cattggcgaa aatacccaac tgaccgctgg
     6061 caaccagatc tttgatcttg ttctgaactt tggtgaactc ttccggactg ttcaggtgcc
     6121 aggtcgaaac acctttcagc atttcggagg ctttggttgg gtcagcttgc agtgcagaag
     6181 tgatgtccac ccagtccagc gccgaaagct gatagaaatg aacgatatgg tcatgcgtgg
     6241 tgtgcgcagc cagaatgatg ttacggatgt attgcgcgtt aaccggaacg tcgatattca
     6301 gcgcactttc tgccgcacga acggaagaca gcgcgtgagt ggtagtacat acgccacaga
     6361 tacgttgcac aatcatccat gcatcgcgcg gatcgcggtt tttcacgatc tcttccatgc
     6421 cgcgccacat ggtaccggaa gcccatgctt tcgaaacgac gccattttcg atttcgcaat
     6481 cgatgcgtaa atgcccctca atacgggtta ccggatcaat agtaattctc tggctcatgc
     6541 tttgctcgcc tcatgacgat tatgatcgtt ttgttttaaa ggaggaagta tcggcagtag
     6601 acgaatgagt acgatgtaag cgcaaatctc aatagccaca aaaccaatag aaatcaacag
     6661 ttcttcccag gtcgggaagt aggcgtaacc gccgcccggg ttgaatgcca ccagcgaata
     6721 ggtcagacgc caggttgcac aacctaacag cgcgctcagt gctgacagga acagcatgcg
     6781 ggaatcatta cgcagcttcg ccacacgcag aacgaccagc gggaagagca tcagcaggac
     6841 ttcaatccag aacatcacgg agtagaagtc accggcaaac gctaacgaca gcttgtcgcg
     6901 atagataagc tcgccaaagc gcagcacgat gaaaatcgcc agcaacacac tgatggtgtt
     6961 ggtcagcttg acgaataggc tcttttcatc cggaccgttg ccacgcagac ccgcctgcac
     7021 cagtgaacct tcaaagatga caatcgagaa gcccatgatg aacgccgtca gcagcgagaa
     7081 cagcggcaac atttcatagc tctgccacag cggatgcacc ttgtagcccg ccgagatcat
     7141 cagcgacccc attgaagact ggtgcatggt cggcagcagc gcaccgagcg cgatgatgaa
     7201 gaacatcacc ttgttcagac gctgtagcga caccttccag cccagacgtt caaacagtgc
     7261 cggagcaaac tccagagcca tcacgccgat gtagatggtc atacagaccg ccgtctcgaa
     7321 cagtaccgag ttcacgttga agtgacccgg aatgtagaag tacggcaggt tccagtagcg
     7381 acccacgtcg atagtgatcg acaagccacc cagtgagtaa ccaaacagac tcgccaacag
     7441 cgccggacgc accagcggat ggtattgccc acggttaaag acgtataccg cccacgcaag
     7501 cgcccagccg ccacaggcaa agccggtgcc aatcagcagg tcaaacgcga tccacacgcc
     7561 ccacgggaag ccgccgttca ggtcagagac agagcccaga ccgaacacca gacgcttcac
     7621 aatcaggagc atacagatga cgattaacgg tccaaaaatc atgaccggtt tactgatgat
     7681 tttgccgccc agcggttgtg gatcatgact catgatcgtc tcctccgtcg tgatggtcgt
     7741 ttttggtgtt gcgacgaacc agcacggtca agcccgccag cacagccagt ggtagcatca
     7801 tgcctttata cagggtgtgt tgaatatttt cggaacgcgc accggtagaa agatcgtcca
     7861 gtttcggcag gtcgagattt tcataaggca cacccgtcag taccagaacc tgagtaccgc
     7921 cgccctcttt ctcgccgtac agatgcggat aatatttcgg caccgtatgc aggtaagtgt
     7981 cgccagattt cagcgtctga cgcggatagt ggtattcgct gccaggcttc agcgccagac
     8041 gttttttcgc ctccgccatc agctcttcac gcgtaccaaa aatcaccgcg cccgccgggc
     8101 acacttctac gcagccaggc agaccgcctt tatcgagacg ttccacacct ttctggttgc
     8161 acagctcgca cttatgcagc gcaccaaacg ggttgttgta gtcgtacttc ggcacgttgt
     8221 acggacaggc gaccatgcag taacggcaac cggtgcacac gtctttgtcg taatggacaa
     8281 tgccggtttt cggatctttt ttcagtgcag agaccgggca cacagagaca cagttcggat
     8341 cgacgcagtg catacactgt ttcttaatgt acgcgtagcc gttctcctcc tggtctttgt
     8401 tgacccctgt gccgctggtc cacacttgaa tgatgttatt ggtatacggc gacagtttgt
     8461 cgttgttcga ccaggtctgt tccccttgcg ggttacgttc agggaagttg atatcctgac
     8521 acttggtgac gcaagcctgg cagcctacgc acaaggtcga gtcgtacaac atccccagcg
     8581 atcccggaat tggcgggcgg ttttcagcag ccgcatgact gacagacggc agcgcgcccg
     8641 tcagcaatgc cccgcaggag gctgctttaa taaaattacg tctgttcacg gttattctcc
     8701 ccgtgagtca gcgttatctt tcttttgctg acgacccagt tcacgcaccg ccatcacgct
     8761 gacaccggca accagcccaa ccacaccgcc gagcaaacca atagcgcctg cagagacgtt
     8821 gccgccctct ttagcgttaa catccggttt ctgtgaacgc ggagtctgat tttcgacgtt
     8881 ggcaagctga tggatgcctt tatggaagcc gataccttct tcgttacagc catagcacgg
     8941 gtgaccaatc gccaccggcc acacaccgcc aacatcgcag aattgcagcg ttgagcagtt
     9001 gccgtaagtt tctggccctt tacagccgag gtggtacagg caccagcctt cgcggtggcc
     9061 ttcatcaccg aactctttgg caaaacgacc agcatcgaag tgcgggcgac gttcgcagtg
     9121 ttcgtgaatc agacggccat aggcgaaggt cggacggttt ttgtcatcca gtttcggcgg
     9181 tttgccgtaa gtgatgatgt gcgcaacggt cgcgaggaag ttgtgcgggt tcggcgggca
     9241 gcccggaata ttgataacgg ttttgcctgg cagaacttct tgcaagctga ctgcgccagt
     9301 tgggttaact ccagctgcgg caacaccgcc ccacgcagag caggaaccga tagcgataat
     9361 ggctgctgcg ccttccgccg ctttgcggat gtgatccaca atcggctcac cggcaaccat
     9421 gcaataaata ccgttatctt ttaatgggat ggaaccatcc accactaaca catactgccc
     9481 tttgtacttc tcgagagcgt tatgtttgtt ctcttcgacc tgatgaccga aggcggcgga
     9541 aagcacttcg tgatactcca gagagatagt ctccagtacg aggttttcta ccgttggatg
     9601 cgttgcacga agcagagatt ccgtacaacc ggtgcactcc tgcgcgccaa tccagataac
     9661 tggcggacgc tgcgggttag taaccgattc ggccatctct gcagcggctt tgctacttaa
     9721 ccccatggtg gcggctaatg ctgcacaaag cttcatgaaa tcacgacggt taatgccgtg
     9781 agaatggatg agggtgttat ctccagtcat ttatagttat tccgttgcga agacctggca
     9841 tatattttgc ctcaatcgca aaatcaataa tgcgatcgat gcgccattta ccacacattt
     9901 attatggtta tcgatatcat gatactgcgg cggggcgcta aaacgaaggg aagaagcatt
     9961 agtgtaatta attaaagcaa gataatacgt atgtttgatc aattttcgag gcgataaccg
    10021 gccataaaaa agcgcaacgt agaacaggaa ttattctcac gaaagaaatc cattaccatc
    10081 tctttatcca gtccatagcg gcgcgtcaga atacccgctt cccaggcgct aagctggcga
    10141 tctccggtct tttcgtacat ccggtgtgag tagcctaata caaaaccgcg tttataatcg
    10201 gcacagaatt ttgtcacatt aaccgcactg tcggcatgcg ttgcttttaa tcccgccatt
    10261 aagcctttac caaaatggtt attcatcgtt cacctcattc gctatatatc gtaatatata
    10321 gcgatatttt aggcaaagaa cgacttttgc gcacgctatt ttttcatgct tgtttaagac
    10381 gtaatcccgt aagagataaa cctatcagga gagatattta tcgaaccatt tcagtgtcct
    10441 ttgccaggca agatcggcgg cagatttgtc ataacggggc gtggaatcat tatggaatcc
    10501 gtgattaacc cccggataga tatacgcctc ataaacctta ttattggctt tcaacgccgc
    10561 ctcgtaagca ggccagccct cgttgattcg ggtgtccagt tccgcgaagt ggagtagtaa
    10621 aggcgcttca atcttcgcca catcggcagt gggtgcctga cgaccataaa acggcactgc
    10681 gcaggccagt tccgggtaag cgaccgctgc cgcgttcgat acgccaccgc catagcaaaa
    10741 tccggtaata cccactttgc ctgtcgcttg cggatagcgt tgcataaact caattgcggc
    10801 aaagaaatca ttcatcagtt tggttggatc aacctgctgt tgcagctcac gacctttatc
    10861 atcatttccc ggataacctc caacggaatt taagccgtca ggtgccaggg cgatataccc
    10921 cgccttcgcc actcgccgtg ccacatcttc gatatacgga ttcagtccac gattctcatg
    10981 caccaccacc acggctggcg ttttgccgct catttttgcg ggtttaacca gataaccccg
    11041 tacctcgccg tgaccatttg gcgaaggata cgtgatgtac tcagcaaaaa tctccgggtc
    11101 ggtaaactct acctgagtcg ccagcgcata atttggcttg agcaaatcaa acaacgctaa
    11161 tgccgtcatc ccgcctaccg catacttcgc cgcaaggttg aggaactcac gtttcgagat
    11221 tttcccgtga gcgtaatagt cgtagtaatc cagcaactct tgtgggaaat ctttggcggt
    11281 taaacgcggc atcgttgcac tcctcagttg gtgttttttt aagcaaagca taagcacgtg
    11341 tttttgccca gtttttcgtc actctgtgag ccagactacg ggatacgcgc tggcgaatcg
    11401 ctaaactaga aacattgttt cgaaattgaa cggtggaaag gagaggtcat ggtctggtta
    11461 gcgaatcccg aacgttacgg gcagatgcaa taccgctatt gcggaaaaag cggtttacgc
    11521 ctgcccgcgt tatcgctcgg tttatggcac aatttcggtc acgttaacgc gctggaatca
    11581 cagcgtgcga tcctgcgtaa agcgtttgat ttaggcatta cgcactttga tttagccaac
    11641 aattacgggc cgcctccagg aagcgcagaa gagaactttg gtcgcctgct gcgggaggat
    11701 tttgccgctt atcgcgatga actgattatc tctaccaaag ctggctacga tatgtggccc
    11761 ggcccttacg gctctggcgg ttcacgtaaa tacctgctcg ccagcctcga ccaaagcctg
    11821 aagcgtatgg ggcttgagta tgtcgatatc ttttactctc atcgcgtcga tgaaaatacg
    11881 ccgatggaag aaaccgcctc tgcgctggct catgcggtac aaagcggtaa ggcgctgtat
    11941 gtcgggatct cctcttactc accagagcgg acgcaaaaaa tggtcgagtt gctgcacgag
    12001 tggaaaattc cgctgttaat tcatcaacct tcgtacaatt tactaaaccg ctgggtggat
    12061 aaaagcggcc tgctggatac cctgcaaaat aacggcgtgg gctgcattgc ctttactcct
    12121 ctggctcagg gattgctgac cggaaaatat ctcaacggca ttcctgaaga ttcacggatg
    12181 catcgtgaag ggaataaagt tcgtggtctg acgccgaaaa tgctcaccga agccaacctc
    12241 aacagcctac gcttattgaa tgaaatggca cagcagcgtg gacaatcaat ggcgcaaatg
    12301 gcgttaagct ggttgctgaa agatgagcga gtgacgtcgg tattggttgg tgccagccgc
    12361 gcggagcaac ttgaggagaa cgtgcaggcg ctgaataatc tgacatttag caccgaggag
    12421 ctggcgcaga tcgatcagca tatcgccgat ggcgagctga atctgtggca ggcgtcttcc
    12481 gataaatgac ctgttaataa cgggcgcgag aaccgcgccc gaatcagatc agtgattatg
    12541 acgagtcagt cggtcaagat agcccattac aaatgcagag agcacaaacg tcagatggat
    12601 aatgacgtac cacatcagtt tgttatcagg gacatttttc gcatccataa agacgcgcag
    12661 taagtgaatg gaagaaattg ccacaatcga cgctgctact ttgtttttca gcgacgttgc
    12721 atccattttc cccagccagt tcagcttctc tttgttctcg gagatatcga gttgtgagac
    12781 gaaattctca taaccggaaa acatcaccat caccagcaaa ccgccaacca gcgtcatatc
    12841 caccagcgac agcaacacga ggatcaaatc tgattccgcc atcgagaaga tattcggcag
    12901 tacgtgaata atctcctgga agaacttcag cgccagggca actaacgcca gcgaaaggcc
    12961 aaagtacacg ggggcaagca gccagcgaga agcatacatt gcattttcaa gaaaacgttc
    13021 catacattcc tgtcgtgtag ttaaaacggg caacagtata tcgcaattga gtaaacagcg
    13081 cgcaacagca aatcaacata tccgttaaat ctttgtcgca taattctgag ggttagggat
    13141 cttcctggtt cccttatcaa cgcatcgaga gcgtctaccc ttaaaggacg ttgaacctga
    13201 agggagaaaa acgatgtctc atttaaaaga cccgaccacg cagtattaca ctggtgaata
    13261 tcccaaacag aaacaaccga cgccaggcat ccaggcgaag atgacaccgg taccggattg
    13321 cggcgagaaa acctatgttg gtagcggtcg cctgaaagat cgtaaagccc tggtgacagg
    13381 gggcgattcc ggaatcggtc gcgctgccgc catcgcttac gcgcgtgaag gggctgacgt
    13441 ggcgatcagt tatcttcccg tggaagaaga agacgctcag gatgtgaaaa agatcattga
    13501 agaatgcgga cgcaaagccg ttctgctgcc aggcgattta agcgatgaga aatttgcccg
    13561 ttcgctggtt cacgaagcgc ataaggcctt aggtgggctg gatattatgg cgctggtcgc
    13621 cgggaaacag gttgccattc ctgatattgc agacctcacc agcgaacagt ttcaaaagac
    13681 ctttgccatt aacgttttcg cgctgttctg gctaacccag gaagcgatcc ccctgctacc
    13741 gaaaggtgca agtattatca ccacttcgtc aatccaggca taccagccaa gcccgcattt
    13801 actggactat gcagctacga aggcggcgat tctgaactac agccgaggcc tggcaaaaca
    13861 ggtcgcggag aaaggtattc gggtgaatat tgtcgcgcca ggcccgatct ggacagcact
    13921 gcaaatttcc ggcggacaaa cgcaggataa gatcccgcag tttggtcagc aaacgccgat
    13981 gaaacgtgcg gggcaaccgg cggaactggc ccctgtatat gtttatctgg caagtcagga
    14041 gtcgagctac gtcaccgcag aagtgcacgg cgtgtgcggc ggcgagcatt taggttaaaa
    14101 aaatgcccgg ttgtgaaaag caaccgggca tcattgtgaa ttcactctta cctgatgtgg
    14161 cgaaaacgcc ttatccgcct acaaatgcat gaagattcaa catgttgcaa tagattttgt
    14221 aggcctgata agcgtagcgc atcaggcatt gagcacctaa tgccggatgc ggcgtgaacg
    14281 ccttatccgg cctacaaacg cacgaagatt caacatgttg caatagattt tgtaggcctg
    14341 atgaacgtag cgcatcaggc aattctactt tacttcgctt tggcggtttc ttcgccgacc
    14401 agacctatct tcaggtaacc cgcctgatgc agcgtgtcca ttaccttcat caacgtctcg
    14461 taatcgacag ttttatccgc tcggaagaag atggtggtgt ctttcttgcc ttcggttaac
    14521 gcattcaacg ccgtaatcat tgtttcatcg gtgaccggat cgttaccgat aaacatcgag
    14581 ttgtctgcct tcaccgacag ataaaccggt ttttccgggc gcggctgcgg cgtgctggta
    14641 gaagcaggca agttaacctt cacatctacc gtcgctaacg gtgccgccac cataaagata
    14701 atcagcagga ccaacatcac gtcgataaac ggcgtcacgt tgatatcatg catttcgccg
    14761 ttatcgtcga ggttttcgtt aagatgcatt gccatcggac attatcctgc gcgtaatttt
    14821 tgtgcgacac gaaccggatg cgcagcggcg ctggcttcca gatccaggtc acggctttgc
    14881 agcaacaata cctgcgctgc gacatcaccc agcatcgctt taaagccgcc aatctgacgc
    14941 gcgaatacgt tatagataac gaccgccgga atcgctgcca ccagaccgat tgccgttgct
    15001 aacagagctt ctgcgatacc cggcgcaacg actgccaggt tagtggtctg cgtttgcgcg
    15061 ataccaataa agctgttcat gatgccccag accgtaccaa acagcccaac gaacggcgaa
    15121 atcgcgccga tggttgccag atagccgtta ccgcggccca tttgacgacc tactgcggcg
    15181 acccgacgct ccagacggaa gctggtacgt tctttaatac cttcgttatc gtcgctgcct
    15241 tctgacagtt ccagctcatt ctgcgcttca ttgagcaaat gcaggcttaa gcttttgcta
    15301 ccaaaatcag cggcgatatc gttggcctgg tttaaggaac gcgcttcagc cagcagttgc
    15361 tgctcgcgct taaggcgacg cttctgatta aagaactcta cgctcttact gaagaagatt
    15421 gcccaggtga ctacggaggc caaaataagc ccaatcatca cgcacttaac gacaatatcg
    15481 gcgtgctgat acatacccca gacggaaagg tccgtctgca ttaaattatt acccactgtg
    15541 tatctccagg acgcaagtca caaaatctgc gcataataat atcaaaacga cgtcgaattg
    15601 atagtcgttc tcattactat ttgcatactg ccgtaccttt gctttctttt ccttgcgttt
    15661 acgcagtaaa aaagtcacca gcacgccatt tgcgaaaatt ttctgcttta tgccaattct
    15721 tcaggatgcg cccgcgaata ttcatgctag tttagacatc cagacgtata aaaacaggaa
    15781 tcccgacatg gcggacaaaa agcttgatac tcaactggtg aatgcaggac gcagcaaaaa
    15841 atacactctc ggcgcggtaa atagcgtgat tcagcgcgct tcttcgctgg tatttgagag
    15901 tatggaagcc aaaaagcacg cgacgcgcaa tcgcgccaat ggcgagttgt tctatggacg
    15961 gcgcggaacg ttaacccatt tttccttaca acaagcgatg tgtgaactgg aaggtggcgc
    16021 aggctgcgcg ctatttccct gcggggcggc agcggttgct aattcgattc ttgcttttgt
    16081 cgaacagggc gatcatgtgt tgatgaccaa caccgcctat gaaccgagtc aggatttctg
    16141 tagcaaaatc ctcagcaaac tgggcgtaac gacatcgtgg tttgatccgc tgattggtgc
    16201 cgatatcgtt aagcatctgc agccaaacac taaaatcgtg tttctggaat cgccgggttc
    16261 catcactatg gaagtccacg acgttccggc gattgttgcc gccgtgcgca gtgtggtgcc
    16321 ggatgccatc attatgatcg acaacacctg ggcagccggt gtgctgttta aggcgctgga
    16381 ttttggcatc gatgtttcta ttcaagccgc caccaaatat ctggttgggc attcagatgc
    16441 gatgattggc actgccgtgt gcaatgcccg ttgctgggag cagctacggg aaaatgccta
    16501 tctgatgggc cagatggtcg atgccgatac cgcctatata accagccgtg gcctgcgcac
    16561 attaggtgtg cgtttgcgtc aacatcatga aagcagtctg aaagtggccg aatggctggc
    16621 agaacatccg caagttgcgc gagttaacca ccctgccctg cctggcagta aaggacacga
    16681 attctggaaa cgagacttta caggcagcag cgggctattt tcctttgtgc ttaagaaaaa
    16741 actcaatgat gaagagctgg cgaactatct ggataacttc agtttattca gcatggccta
    16801 ctcgtggggc gggtatgaat cgttgatcct ggcaaatcaa ccagaacata tcgcagccat
    16861 tcgcccacaa ggcgagatcg attttagcgg gaccttgatt cgcctgcata ttggtctgga
    16921 agatgtcgac gatctgattg ccgatctgga cgccggtttt gcgcggattg tataacattg
    16981 ccacttttgg acaattttgc agacatttta ttgtgaaaag tcttaaattg ttgcgtccgg
    17041 gatcaaggcg tcccggacga ttcaggagta caataggcag ataaaggctt aaacgctgtt
    17101 ccacaggaaa gtccatggct gttattcaag atatcatcgc tgcgctctgg caacacgact
    17161 ttgccgcgct ggcggatcct catattgtta gcgttgttta ctttgtcatg tttgccacgc
    17221 tgtttttaga aaacggcctg ctgcccgcct catttttgcc aggcgacagc ttgttgatat
    17281 tggctggcgc attgattgcc cagggggtta tggattttct gcctacgatt gcgattctga
    17341 ccgccgcagc aagtctgggc tgctggctaa gttatattca ggggcgctgg ttagggaata
    17401 ccaaaacggt gaaaggctgg ctggcacagc ttcctgctaa atatcaccag cgcgccacct
    17461 gcatgtttga ccgccacggt ctgctggcgc tgctggctgg acgttttctt gcatttgtcc
    17521 gtacgctgct gccaaccatg gcgggaattt ccggtctgcc aaaccgccgt ttccagtttt
    17581 tcaactggtt aagcggattg ctgtgggtca gcgtggtaac cagtttcggc tatgccttaa
    17641 gtatgattcc gttcgttaaa cgccatgaag atcaggtaat gacgttcctg atgatcctgc
    17701 caattgcctt gttaaccgct ggcttgttag gcacgctgtt tgtggtgatt aaaaaaaaat
    17761 actgtaacgc ctgacgattt tccctgttcc cggttgctgt accgggaacg tatttaattc
    17821 ccctgcatcg cccgcattct tgccgcatct tcccccggcg tcacaccgaa gtaacgttta
    17881 aactcacggc taaattgcga tgcgctttca tagccgacgc gcatcgctgc tgcgctggcc
    17941 ttcatgccgt catggatgat catcatccgc gccttatgca gacggtaatt cttcaaatac
    18001 tgcaacggcg aggtgctggt gacagactta aaattatggt ggaacgccga tacgctcata
    18061 ttagcttctg ccgccagttg ctcgacgctc aggttttcgg tgtatttatt ctcaatccgt
    18121 ttcagcacgc ggctaatcag actgaagtga gtctggcgac tgaccagcgc cagtaacgcg
    18181 ccgccgcaag gtccggtcag cacgtagtac agaatttcgc ggatgatctg tttgccgaga
    18241 atacgcgcat ccagtggtcg cgccatcacg tcgagtaacc gctccgccgc gcataaaatc
    18301 tcttctgata acgtggcgga gttaatcccg ctggctgcca tcgacggctg gaaatgctca
    18361 tcttcgccaa tatccatcaa cagttcctgt aactgcaaaa tatcgacatt gagacgcaac
    18421 cctgccagcg gcacctctga cgtggcatag gtttcgcact caaacggcaa cggcaccgtc
    18481 agcagcaggt attcattggc atcataacga aacacgcgtt cattgatata accgatttta
    18541 tgtccggaaa agagaattat gatgccaggc tcgtacatca ccggtgtacg tgcgaaaggc
    18601 gtctcgccat acaacaaacg cacatcgggc aacagttctg acaaactatt ttctttattt
    18661 ttcagtttat taactttatc cgccagcaag cggcaaatct cttcacgttt catatcgcgt
    18721 aatttcttag gaataatgcg gcaatttgat tgtgcgcaat tttgtagcat ttctccagca
    18781 ctctggagaa ataggcaaga cattggcaga aatgagcatt gagagccagg gcgctggcga
    18841 tcacaatgaa aaacatcagg cagatcgttc tctgccctca tattggccca gcaaagggag
    18901 caagtaatga acaactttaa tctgcacacc ccaacccgca ttctgtttgg taaaagcgca
    18961 atcgctggtt tacgcgaaca aattcctcac gatgctcgcg tattgattac ctacggtggc
    19021 ggcagcgtga aaaaaaccgg cgttctcgat caagttctgg atgccctgaa aggcatggac
    19081 gtgctggaat ttggcggtat tgagccaaac ccggcttatg aaacgctgat gaacgccgtg
    19141 aaactggttc gcgaacagaa agtgactttc ctgctggggg ttggcggcgg ttctgtactg
    19201 gacggcacca aatttatcgc cgcagcggct aactatccgg aaaatatcga tccgtggcac
    19261 attctgcaaa cgggcggtaa agagattaaa agcgccatcc cgatgggctg tgtgctgacg
    19321 ctgccagcaa ccggttcaga atccaacgcc ggtgcggtga tctcccgtaa aaccacaggc
    19381 gataagcagg cgttccattc agcccatgtt cagccggtat ttgccgtgct cgatccggtt
    19441 tatacctaca ccctgccgcc gcgtcaggtg gctaacggcg tagtggacgc ctttgtacac
    19501 accgtggaac agtatgttac caaaccggtt gatgccaaaa ttcaggaccg tttcgcagaa
    19561 ggcattttgc tgacgctgat cgaagatggt ccgaaagccc tgaaagagcc agaaaactac
    19621 gatgtgcgcg ccaacgtcat gtgggcggcg acgcaggcgc tgaacggttt gattggcgct
    19681 ggcgtaccgc aggactgggc aacgcatatg ctgggccacg aactgactgc gatgcacggt
    19741 ctggatcacg cgcaaacact ggctatcgtc ctgcctgcac tgtggaatga aaaacgcgat
    19801 accaagcgcg ctaagctgct gcaatatgct gaacgcgtct ggaacatcac tgaaggttcc
    19861 gacgatgagc gtattgacgc cgcgattgcc gcaacccgca atttctttga gcaattaggc
    19921 gtgcctaccc acctctccga ctacggtctg gacggcagct ccatcccggc tttgctgaaa
    19981 aaactggaag agcacggcat gacccaactg ggcgaaaatc atgacattac gctggatgtc
    20041 agccgccgta tatacgaagc cgcccgctaa gctttttacg cctcaaactt tcgttttcgg
    20101 gcatttcgtc cagacttaag ttcacaacac ctcaccggag cctgctccgg tgagttcata
    20161 taaaggagga acgtatggct aatccaaccg ttattaagct acaggatggc aatgtcatgc
    20221 cccagttggg actgggcgtc tggcaagcaa gtaatgagga agtaatcacc gccattcaaa
    20281 aagcgttaga agtgggttat cgctcgtttg ataccgccgc ggcctataag aatgaagaag
    20341 gtgtcggcaa agccctgaaa aatgcctcag tcaacagaga agaactgttc atcaccacta
    20401 agctgtggaa cgacgaccac aatcgccccc gcgaagccct gctcgacagc ctgaaaaaac
    20461 tccagcttga ttatatcgac ctctacttaa tgcactggcc cgttcccgct atcgaccatt
    20521 atgtcgaagc atggaaaggc atgatcgaat tgcaaaaaga gggattaatc aaaagcatcg
    20581 gcgtgtgcaa cttccagatc catcacctgc aacgcctgat tgatgaaact ggcgtgacgc
    20641 ctgtgataaa ccagatcgaa cttcatccgc tgatgcaaca acgccagcta cacgcctgga
    20701 acgcgacaca caaaatccag accgaatcct ggagcccatt agcgcaagga gggaaaggcg
    20761 ttttcgatca gaaagtcatt cgcgatctgg cagataaata cggcaaaacc ccggcgcaga
    20821 ttgttatccg ctggcatctg gatagcggcc tggtggtgat cccgaaatcg gtcacacctt
    20881 cacgtattgc cgaaaacttt gatgtctggg atttccgtct cgacaaagac gaactcggcg
    20941 aaattgcaaa actcgatcag ggcaagcgtc tcggtcccga tcctgaccag ttcggcggct
    21001 aacgtgcaaa ttctcccggt ggcggtaatg ttccgctacc ggacttttca gaaatcattt
    21061 attcccctcg cgtcccgccc gttgttactc ttccttgttc aggaatgcca aatataagga
    21121 catcatcatg cagagccgga agctcttaaa agaacaactc atctatatcc gggataaacg
    21181 caacggagag gtgaaaaaca gatgaaaata atacttctgt ttttagcagc cctggcaagt
    21241 tttaccgtgc acgcacagcc cccctcacag accgtagaac aaacagtccg gcatatttat
    21301 cagaactata aatcagatgc cactgcccct tattttggtg aaaccggaga gcgtgcgata
    21361 acttctgtgc gtattcaaca ggcgcttacc ctgaacgaca atcttacgct gccgggtaat
    21421 attggctgga ttatgatccg gtttgtgatt gtcaggattt tggcgatctg gtgctagaaa
    21481 gcgtagcgat aaccgaaact gacgccgatc atgccgatgc cgttgtgcgc tttcgtatct
    21541 ttaaagatga taaagaaaag accacgcaga cactgaaaat ggtggcggaa aatggtcgtt
    21601 gggtcattga cgatattgtc agcaatcatg gcagcgtctt acaagcagtt aatagcgaga
    21661 atgaaaaaac gctggccgct ttagcttcgt tgcaaaaaga acagccggaa gcctttgttg
    21721 ccgaactctt tgaacatatt gccgattata gctggccgtg gacgtgggtg gtttccgact
    21781 cttaccgcca ggcggtcaat gccttctata aaaccacctt caagacggcc aacaatcccg
    21841 atgaagatat gcaaatagaa cggcaattta tttacgacaa tccgatctgt tttggcgaag
    21901 agtcgctatt ttcacgcgtt gatgaaattc gagtcctgga gaaaaccgcc gattccgccc
    21961 gcattcatat tcgttttacg ctgaccaatg gcaacaacga agagcaagaa ctggttttac
    22021 aacggcgcga aggtaagtgg gaaatcgctg attttatccg cccgaacagc ggcagcctac
    22081 ttaagcagat tgaggcaaaa accgccgcca gattaaagca atgagctgaa ttaaatagca
    22141 attagccgga acaataaaaa agggaacact atatgaaaac gattttcacc gtgggagctg
    22201 ttgttctggc aacctgcgtg ctcagtggct gcgtcaatga gcaaaaggtc aatcagctgg
    22261 cgagcaatgt gcaaacatta aatgccaaaa tcgcccggct tgagcaggat atgaaagcac
    22321 tacgcccaca aatctatgct gccaaatccg aagctaacag agccaatacg cgtcttgatg
    22381 cacaggacta ttttgattgc ctacgctgct tgcgtatgta cgcagaatga taaaaaaatc
    22441 cccggcagca tgtcagttgc cggggatttt ttttaacgtc caaccgccgc tttagggcgt
    22501 ttcttcgcac cagcattcac cggacgagat tgcgtagacg acgctttttt tgccgtagca
    22561 ggcgtctgac actgggtcgc catcggcgta tgtttcgtca acgccggacg ggtattgcgg
    22621 ttctggcgac gagcctcacg catctcttca atggttggcg caggcactaa gcaatcgcga
    22681 cggctgccaa tcagatgctt tttgcccatc gcttccagcg cctggcggat taacggccag
    22741 tttgccggat cgtggtaacg caacaacgct ttatgcaaac gacgctgttt gtcgcccttc
    22801 ggtacgaaga cgtcttcact cttataacca atcttcgcca gcgggttttt cccggtgtaa
    22861 tacatggtgg tcgagttagc cagtggcgat gggtagaagt tctgtacctg gtcgagacgg
    22921 aaacgatgct ttttcagcca cagcgccaaa ttcaccatat cttcatcacg cgtaccgggg
    22981 tgcgcggaga tgaaataggg gatcagatac tgctctttac ctgcctgttt cgagtaggta
    23041 tcgaacagct ctttaaagcg gtcataactg cccatgcccg gcttcatcat cttcgacaac
    23101 ggcccttctt cggtatgttc cggagcaatc ttcagataac cgccgacgtg atgggtcgcc
    23161 agttctttga tatagcgcgg atcttccacg gcgatgtcat aacgtacgcc tgaggcaatc
    23221 agaatctttt taatgccttt cagatcgcga gcgcggcgat agaggttgat cgtcggttcg
    23281 tggttagtgt ccatatgcgg acaaatatcc gggtaaacgc aggacaaacg acggcaggtt
    23341 tgttcagcgc gtggcgattt gcagcgcaac atatacatgt tggcagttgg cccaccgaga
    23401 tcggaaatta cgcccgtaaa acctggaacg gtgtcgcgga tcgcttcgat ctcattaatg
    23461 atcgaatctt cggaacggct ctgaataatg cgcccttcgt gctcggtgat agaacagaaa
    23521 gagcagccgc caaagcagcc gcgcataatg ttgaccgaaa aacggatcat ttcgtaagcc
    23581 ggaatacggg cattgccata ggccggatgt ggcacgcgct tgtacggcag cgcaaaaacg
    23641 ctgtccatct cttcggtaga aagcgggata gcaggcgggt tgatccacac atagcggtca
    23701 ccgtgttttt gcatcaatgc gcgggcgcaa cctgggttag tttcgtggtg cagaatacgc
    23761 gaagcatggg cgtacagcac tttatcgccc ttcactttct cgaaagaagg cagcaacacg
    23821 taggtttttt cccacggttt cgggcgcggt ggctgcacgg ttacggcttt ggcttcctgc
    23881 tttttcggtg ccaccggttt gttatccgcg cacggcaaat cttcaccata cggatgcggg
    23941 attgggtcga tttttccagg ggtatcaaga cgggtggaat ccacgccgct ccagccaggc
    24001 agcgcctctt tcacgataat cgcggtatta cgcacatcgc ggatttcact aatcggctcg
    24061 cccatcgcca gacggtgcgc cacctccacc agcggacgct caccgttacc aaacatcagc
    24121 atgtcggctt tcgaatccac cagcacggaa cggcgcacgg tatcggacca gtaatcataa
    24181 tgcgcggtac ggcgcagact agcctcaata ccaccgagga tcaccggtac atctttccac
    24241 gcctctttac aacgctgggt ataaaccagt gtggcgcgat ccgggcgctt acccgcgacg
    24301 ttatccggcg tgtaggcatc gtcatgacgt aaacggcgat cggcggtata acggttgatc
    24361 atcgaatcca tgttgccagc agtaacaccg aaaaacagat tcggtttacc cagacgcatg
    24421 aaatcgtctt tgctgctcca gtctggctgg gcgatgatcc cgacgcgaaa gccctgtgct
    24481 tccagcatac gaccgcaaat cgccatcccg aagcttgggt gatcgacata cgcgtcgcca
    24541 gtaaccaaaa tgatgtcgca gctatcccag ccaagttgat ccatctcttc acgagacatc
    24601 ggcaaaaacg gtgccggtcc aaaacaggcg gcccagtact gcggccagga gaacaggtcg
    24661 cgatccggtt ggatcaggga gatagagctc attttgcttc cagaaatgat aaaaaaataa
    24721 tcaaaggccg gggattataa gccggaacga aagagaaatc gaaaagtatt ccatactcgc
    24781 cctcctcggg cgagtatgaa gattacggta ccggattgac taacagttgc ccaatcgagc
    24841 cacggtccgc catttccagc gtctgactgt tgaagtagaa cgggaagtgc gcccaggaag
    24901 gctgaccgaa ataaacaagc agctccactt gtccgtcaac ccaaacggta tctttccagc
    24961 ctctgtcttc cgggaacggc atcgcaccat tcacgttacg gatctggaac atcacgcctt
    25021 caatatggaa cgcttgcggc tcgtccgcgc gtaccgtcca gcgttcccac gttccttgct
    25081 gcgcggtgac atcaatacgg ttgacgtccc acagttgtcc attaataccc gggtcatcac
    25141 ccagactgat atcgcgactg cgaatcggcg aaccggccat gatttcagtt ggcagcaagc
    25201 gcatcggaag actgtctgtg accagcggca gaaggccggt tgggcgcagc gttagcacca
    25261 gggtagaaac cagaatgctg gatggctcaa agaagccacg aatacgatca acaatgctcg
    25321 ccgcttcgcc acaggtgatc gacacttcat caccgttgct catatccacc agaatctcgc
    25381 ggcgctcgcc cggtgccaac gaaagttgct tcaccgatac aggagcaggc aggaatccct
    25441 gatcgccaga aatcacatgt aacgggcgac catcgctcat ctgtagttga tagcgacgag
    25501 agttcgacgc gttcagcagt cgcaagcgca cccagccacg cgagacttca acgtacgggc
    25561 tttgtacacc gttaaccagc agcgtatcac caacaaagcc gccgcttccc ggttcgttgt
    25621 attctggcgt accaaagtta tccagccgtt tatcctggat aatgaccgga aaatcatcca
    25681 caccataatg gttggggata ggcagcgact tgctgacttc atcttccacc agccacattc
    25741 cggcaaggcc gttatagacc tgctgagccg tgcggttggg agtattggcg tgataccaca
    25801 gagtagctgc gttctggcga ataggcagta cgggtgccca gtcagcgttt ggcgacatca
    25861 tccgtgccgg accgcccatc agcgggcctg gtacctgtag cccggccacc gtcattgaga
    25921 cattttctgt caggcggttg ctgtaaataa gcttaacatc gtcgcccttc cagacgcgga
    25981 tagtcggccc caggtaacga ccattgattc cccagaccga cgcgcgtgtc cctggcgtaa
    26041 atgaccagtg cgcacgttgt acagtcataa acagcggttg cccacggcga gattcaagca
    26101 gcggcggaac gggtagcggt tgctgttgcc cggctgcgct ggccttcagg ggaacagcgc
    26161 ctgcacaaag tgcaatcccc gatgcctgaa tgaactgacg ccgactgagt gacatatttg
    26221 ctccatgtaa aactggctaa agtaataccg tgcaggaaag tgtttcccgc aaaaatacag
    26281 attgccgact taaacttttc cggcggcttc gcgctctgcg acttctttat cgagctcggc
    26341 gattttttgt tccattatcg aacgacaatg ggcagccagc tcacgaacct gatctttgcc
    26401 atactgactg acgtcaattg gcggcagcat ttcgacaatc accagaccgt tgtgcagtcg
    26461 attaagatta atcttattcg aagttgtgga gacgcacacg ggaataatcg ggacgcccgc
    26521 cgcaattgcc gcgtgaaatg ccccagtctt gaacggtagc aggccgcgac cacggctgcg
    26581 ggtaccttcc gggaacatcc agatggaaat acggcgtttt ttgaaatgat tcactacttc
    26641 cgcaatagtg ccgtgagctt tagtgcgatt gtttctgtcg atcaataagt tgccggttaa
    26701 ccagtacaac tgcccgaaga aggggatcca cagcaagctc tttttaccta ccgtcaccgt
    26761 cggcggttgc acaatgttcg atgctgtcac catgtcatag ttgttctggt ggttagcgat
    26821 atagatagca ttgccgtagc tttcagcatc tgcaggtttg cggcactcaa ctttcaggcc
    26881 gaacagcggc gcaagacgac caaacatatg cccaaaggtg gccacatgtt tcgggttacg
    26941 cgggctgaaa aggcagtaaa tggagccgaa tacacagact aagatgctgt aaatcacggt
    27001 aataataaga cgaaagatat atagcataac accctctgaa ggtctgagag actggcattg
    27061 tacgtcacct gaaatcaggt tagggcattg ttaaaagccg ctctctggaa aagcgtattg
    27121 ttaatcgcaa cgcatgaata aacaacggtt tcaagggaat ttttatcgtt tccgggggtt
    27181 gaatacgctg ccggatgacg acttaacgtt tcatccggcg ttccttgcaa gcgggaggaa
    27241 acagcgccct ccccggcata ttactcttcg ctatcaccgc tgctggcacg gcgaggagag
    27301 tcgatctcaa cacgatcgat acgctgcaaa ccgcgcatca acgtaccgcg gcgtccacgt
    27361 tcgccagtga ctttctgtag ctcttccgga cgcagtttaa ttttgcgttt cccaacatga
    27421 atggtcagcg tgctttgcgg cggcagaacg tacagttgcg ccagaccatc ctcgccacgc
    27481 gcggcttctg ccgatggaat gttgataatc ttgttgcctt tgcccttcga cagctgcggc
    27541 agatcactta ccgggaacat caacatacgg cctgcctgag tgattgccag cagcatatcg
    27601 gaagcatctt caattaccac cggcggcata acatgggcat tttccggtaa ggtgatcaaa
    27661 gccttacctg cacggttacg cgccaccaga tcgttaaagg tgcagacgaa accgtaaccc
    27721 gcatcggaag ccatcagcag tttctgatcg tcgctttcca tcagcatatg gtcaacggtc
    27781 gccccaggcg gcaacgttaa tttgccggtg agcggttcgc cctgaccacg cgccgacggc
    27841 agcgtaatcg gatcaatggc atagctacga ccggtggaat caacaaacac taccggttgg
    27901 ttgctcttac ctttcaccgc cgctttgaag ctatcacccg ctttataatt caggcccggc
    27961 gcgtcgatat catggccttt agcgctgcgt acccagccca tctgcgacag cacaatggtg
    28021 acaggttcag acggcagcat gtcgtgctcg ctcatcgctt tcgcttcttc gcgttcctgc
    28081 aacggcgaac gacgatcgtc accgtaggct tgcgcgtctg cctgcagttc tttcttcagc
    28141 aggttattca ttttacgctc ggaagccaaa atgccctgca actggtcgcg ctctttttcc
    28201 agctcactct gctcaccgcg aatcttcatc tcttccagtt tggcaagatg acgcagtttc
    28261 agttcgagga tcgcttccgc ctgggtttcc gtaaggccaa accgcgacat cagcgccggt
    28321 ttcggttcat cttcattacg aatgatctca atcacttcgt cgatattgag aaacgccacc
    28381 agcaaacctt cgaggatatg caggcgcttg aggactttct ccagacgata gttcagtcgg
    28441 cggcgcacgg tatcgcggcg gaacaccagc cattcggaga gaatttccag caggtttttc
    28501 accgccggac gaccatccag accgatcata ttaaggttaa tgcgatagct cttttccaga
    28561 tcggtggtag cgaagaggtg gttcatcacc tgatccatat ccacgcggtt ggaacgcggc
    28621 acaatcacca gacgggtcgg gttctcgtgg tcagattcat cgcgcagatc gtcaaccatc
    28681 ggcagctttt tgttgcgcat ttgcgcagca atttgctcca gtacgcgcgc acctgaaacc
    28741 tgatgcggca atgcgctgat aaccaccgcg ccatcttctt tcttccacac cgcgcgcata
    28801 cgcactgaac cacgtccgtt ctcgtagatt ttacggatct cggcgcgcga agtgataatt
    28861 tccgcttcag tcggataatc cggcccctgc acgatatcca gcagctgatc gagcgtggtt
    28921 ttcggctggt cgattaatgc gattgccgcc tgagccactt cacgcaggtt atgcggtgga
    28981 atatcggtcg ccatgccgac ggcaataccg gtggtgccgt taagcaaaat gtttggcaga
    29041 cgggcaggta gcattttcgg ctcctgcaaa gtgccgtcga agtttggcac ccagtcagcc
    29101 gtcccctgcc ccaactcgct caatagcagc tcggaatatt tcgacaaccg ggattcggtg
    29161 taacgcattg ccgcgaacga tttcggatcg tccggcgcgc cccagttccc ctgaccatca
    29221 accagcggat aacggtaaga gaacggctgc gccatcagga ccatcgcttc ataacaggcg
    29281 ctatcgccgt gcggatggta tttacccagt acgtcaccga cggtacgggc cgatttttta
    29341 aatttggcgc tggcattcag gcccagttca gacatcgcat acacaatgcg gcgctgaaca
    29401 ggtttcagac catcaccaat aaacggcaac gcacggtcca tgatcacgta catggagtag
    29461 tttaagtagg cgttttccgt aaattcatgt agcgcaaggc gctctgccat atcgctcatt
    29521 aattctgatt cctcaactta ttcgccagcc ttcaaagggg caatattgcc ggagatacta
    29581 cattatctgc cgcgtcgagt cacaggagat cagcgtgaat agcgccgccg gacgtgctcc
    29641 gacggcgata aaggcatcaa tgtgccttga tatacaactc tttgctgtag acataatcct
    29701 ggggattatg cagcggaaaa ccgccaacgt agggtttaag cagtttgatt aacggctgat
    29761 agtagatagg aatcagcggt gcctgctggt tgacgatcac ttctgcctgc tgatacaacg
    29821 cattacgctt tgtcgcatca gtgatctgca tggcctggtt tagtaaggcg tcatactgcg
    29881 catttttcca gtgaccgacg ttttcttcac tatcgctttt gagcgtgttc aggaagctgg
    29941 aagcatcatt gtacgtcgca tcccacgact gccgagacag catgaaatca ccggctcgtc
    30001 gggcatcaag ataggttttc cactccattg tgcgcagcgt cacctgtgca cccagccatt
    30061 ttttccattc ggaagacaac gctatcgcgg tcttttcatg cagatcgtac ttgttgtaga
    30121 acagctcaaa gcgtagcgga tgagaggcgt cgtatcccgc ctgtttcagc aaggcttttg
    30181 ccatcgcgac gcgctcactc attggctttt gcagttcatc gaacgtcgtc gcgctaaagc
    30241 cttttacctc tggcggcgtc agcgtggttg cgggcgttct caaccccagt accttttgcg
    30301 caataagctg tcgatcaacc gtaagatata gcgcccgacg cactcgcaca tcgttaaatg
    30361 gcggtttctc aaggttgaag ttgtaatatt cgctgttcag acgcggaata attcgtatct
    30421 cgccaggcag tgatttttca atggcgggaa tttgctgcgc cggaacccag gtgagatcga
    30481 cctctcccgc gcgatagcgg ttatagccgg tgaccgaatt atccagcgcc agatactcaa
    30541 cctgttgcaa tactgtatgt tgcgcatcgc ggtactttgg atttttgcgt gcagtaatct
    30601 tttcgttaac tacccactga tcaagcacaa aggcaccgtt gtaaaccatg ttctctggct
    30661 tactccagct atcgccatgt ttagcgatga catgatgagg aaccgggaac agcgtcggcc
    30721 aggcgagcat cgtcgtgaac caaggaaccg gctgctcaag cgtaacttca agagtacgat
    30781 catccgtcgc tttgacaccc agcgatgtaa catctgcttt acccgcaaca atagccgcgg
    30841 cattgttaat gtgcgcctgt gccagatagc cagcaaaagg gcttgccgtt ttcgggtcaa
    30901 ccgcgcgctg ccagccgagg acaaaatcct ctgccgtcag aggctgaccg tcggaccact
    30961 gcaaaccgct acgcagatgg aaaatatagc gcctgccgcc gtccagtatc tcccagcgtt
    31021 cagcctgagc gggctgcacc tggccttcac cgtccatcca taccagacct tcaaacagat
    31081 ccagcacaat ctgcgcggca gtattctcct ccaccttttg cgggtcgagc gtacctgggt
    31141 cgctatgatt gttgtaacga aagacttgtt gcggggcgag ctgtgtgttg gtgggaacgt
    31201 cagcagcgta gagaggagcc gcacttacca ggctgaccag ccataataaa tttcgcgtat
    31261 acatggtatt ccttaccgga gaaattataa gcccaacaac aatacgcctg tggcattgtt
    31321 cgctcaaact tatcgcgagt gatttggctc acactccggt aaagattccc catcacgcta
    31381 acggcacata aacattcgtt tccagtcgct gctccgtcac cccttcggca agattggtgt
    31441 agtgaaacag aatcggtgct ttacgcattt tctcgccgct tgcaggcagc cagtggcgaa
    31501 taatgcccca tatcgtgtgt gaaatatcgt ccagctcgcc aacgtggcga gccacggcat
    31561 aacgtccacc ggtaagttca ccattgctca caccataacg attatcggga atcggttcgc
    31621 taacgctgcc gcagatatca aagcgaaacg cttccggtgc ggtggtggca gggtcgtccc
    31681 aggcgacgcc aaaagtctgg ctttggttaa caggagaaag ccccgtctct ttacgccaca
    31741 tgataaattt cgccacgctg tagttgagca actcaggcct acagcggtgc ggcaacatcg
    31801 ccacagggat tgatgggaaa tcgataatgt ttacatccag tgtcaggttt gtcatgttgc
    31861 ctccctgcga aattattccc gggaacgcat cccgggagcg gtaacaatta cggatttact
    31921 ttgcggatct gtttgacgtc aatttcaaca gaattccagt ctttatcgac ttcaccctga
    31981 atctcaaccg tatctttcgg cgtcaccgtc acgccgttcc agcgtttgtg gtcgatatca
    32041 acattgatag taccgctggc atctttgaac acgtagagat cgtcagagat gcgttcaacg
    32101 atattgccgc gcagggttac ccaggtgtcg tcacgcaggg attttgcgct ttctacagtc
    32161 gttacgctgc cgttcggccc ctggaatcct ccggcctgac tttgcgttgc cgatgggcca
    32221 gaaaaaccgc cctgctctgc tgccatcacc ggtgcgctgc acagggccat tactgcgatt
    32281 actgctgcga attttttcat gtttattact ccctttaatg tctgtttccg agcatttaac
    32341 aagatagtcc ttaacaactt cttaagggaa aaaaataaaa tttagtgctg tacagagcgc
    32401 gttacaacac ggtttactag cagcaaatac ggttatcgca gggatgaaaa aatgcgaatt
    32461 ttactgatag aagatgacat gctgattggc gacggcatca aaacgggcct tagtaaaatg
    32521 ggttttagcg tcgactggtt tacacaaggt cgtcagggaa aagaggcgct ttatagcgca
    32581 ccttatgatg cggtgatcct ggatttaacc ttaccaggca tggatggtcg cgatattttg
    32641 cgcgaatggc gagaaaaagg tcagcgtgag ccggtactga tcctgaccgc gcgcgatgcg
    32701 ctggaggaac gtgtagaagg gctgcgtctg ggagctgacg attatctgtg taaacctttt
    32761 gcgttgatag aagtcgccgc caggctggaa gctctgatgc gccgaaccaa cggccaggcc
    32821 agcaacgagc tgcgccacgg taacgtcatg ctcgaccccg gcaaacgtat cgccacgctg
    32881 gctggcgaac ccttaacact gaaaccaaaa gaatttgccc tgctggaatt actgatgcgt
    32941 aacgctggtc gggtactgcc gcgcaaactg attgaagaga aactgtatac ctgggacgaa
    33001 gaggtcacca gtaatgccgt tgaagtgcat gtgcatcatc tgcgacgcaa actcggtagt
    33061 gattttattc gtaccgtgca tggtattggc tacacattag gtgagaaatg aaatttaccc
    33121 aacgtcttag tctgcgtgtc aggctgacgc taatcttttt aattctggcc tcggtgacct
    33181 ggctgctttc cagctttgtc gcctggaaac aaacaacgga taacgtcgat gaattgttcg
    33241 acacccaact gatgctgttt gccaagcggt taagtacgct cgatctcaac gaaatcaacg
    33301 cggcggatcg catggcacag acgccaaata aattaaaaca cggtcatgtt gatgacgatg
    33361 cgctgacctt tgccatcttt acccacgacg gcagaatggt ccttaacgat ggcgataacg
    33421 gagaagatat tccctatagc tatcaacggg aaggttttgc tgacgggcaa ctggtgggcg
    33481 ataaagatca atggcgtttt gtctggatga cctcacctga tggcaaatat cgcatcgttg
    33541 ttggtcagga atgggaatac cgtgaagaca tggcgctggc gattgttgcc gggcaattga
    33601 tcccgtggtt ggtcgcactg ccggttatgt taatcatcat gatggtacta ctgggtcgtg
    33661 aactcgcgcc gctgaacaaa ctggcgctgg cactacgtat gcgtgaccct gactcggaaa
    33721 aaccactaaa cgcgactggc gtacccagcg aagtgcgacc actggttgag tcgctaaatc
    33781 aactgttcgc ccgcacacat gcgatgatgg ttcgtgaacg acgctttacc tccgacgcag
    33841 ctcacgaact tcgtagcccg ttaacggcgc tgaaagtgca aaccgaagtt gcgcagctct
    33901 ctgacgatga tccgcaggcg cggaaaaaag cactgctcca attacattcc gggatcgatc
    33961 gcgctactcg tctggttgat caactgctca cgctatcgcg gctggactca ctggataacc
    34021 ttcaggacgt cgcggagatc ccgcttgaag atctcctgca atcgtcggtg atggatattt
    34081 accacacggc gcagcaggcg aaaattgacg tgcgactgac actcaatgtc cagggcatca
    34141 aacgcaccgg acaaccgcta ttgctaagtt tgttggtgcg aaatttgctg gataacgccg
    34201 tgcgttacag tccacagggc agcgtggtgg acgtcacgct gaatgccgat aatttcatcg
    34261 taagggataa cggccccggt gtgacaccag aggcactggc gcgaattggc gaacgcttct
    34321 atcgcccacc cggacaaacc gctaccggca gtggacttgg gctatcgatt gtccagcgaa
    34381 tcgccaagct gcatggcatg aatgttgagt ttgggaatgc ggaacaaggt ggatttgagg
    34441 cgaaggtaag ctggtaaggg taagactttt gctaaattcg caaaagactt tgcacatttt
    34501 gctaatttca ccgtaccgct ctgtgacgta ctatagtcgg caaacgtctc accttgaggt
    34561 taaaaaatga gcaacatcct gattatcaac ggcgcgaaaa aattcgccca ctccaatggt
    34621 caactgaacg acaccctgac cgaagtcgcg gatggcacac tgcgcgacct cgggcatgat
    34681 gttcgcatcg ttcgcgccga cagcgactac gatgtcaaag cggaagtaca aaactttctc
    34741 tgggctgatg tggtgatctg gcagatgcca ggctggtgga tgggcgcgcc gtggacagtg
    34801 aaaaaataca ttgatgatgt attcaccgaa ggtcacggga ctctgtatgc cagcgatggt
    34861 cgtacccgca aagatccgtc gaaaaaatac ggttccggcg gcctggtaca gggcaaaaaa
    34921 tatatgcttt ctctgacctg gaacgcgcca atggaagcct tcaccgaaaa agatcagttc
    34981 ttccacggcg ttggcgttga cggtgtgtat ctgccgttcc ataaagcaaa ccaattcctc
    35041 ggtatggaac cgctgccgac atttatcgct aatgacgtga taaaaatgcc tgatgttccc
    35101 cgctatactg aagaatatcg caagcatctt gtggaaattt ttggttaact agagctcagg
    35161 ctttagaagg agttaaccat gcttaccgta atcgcagaaa tccgtactcg tcctggtcaa
    35221 catcaccgtc aggcggtatt ggatcagttt gctaaaatcg ttccaaccgt actgaaagaa
    35281 gaaggttgcc acggctatgc gccaatggtg gattgcgctg ctggcgtgag tttccagtct
    35341 atggcaccgg attctatcgt gatgattgag cagtgggaaa gcatcgcgca tcttgaagcg
    35401 catctgcaaa ccccgcacat gaaggcgtat agcgaagccg taaaaggtga cgtgctggag
    35461 atgaatattc gtattctgca gccagggatt taacattcct gaaaagaggc cggtgttgct
    35521 accggcctca ggtcagatta gccattcttt cttcccgttt tcagcccccg atggaaatcg
    35581 ttaattcgct taatggtttt aaccgcacgc tgcggcaccg cggaagccac cgaccagaca
    35641 atcatttcca ggcacaacaa taccgtacca tgtagtggcg ttttcccctt ttcatccccg
    35701 cgcggaacat gaatcacgat actggcgtct ttgctaaaac gtgaatccag cgcgtttgtc
    35761 agcaaaataa cagggatccc cagccgtctc gcttcacgca gcgttgtcag cccttcccgg
    35821 tgcgcagatt tttgcgccat catgatcaga acatcgccac gttgaagcgc aatcagttgc
    35881 tcggcaaggc caatacccgt acggtttagt gccgtggcag gcaaacctat gcggttgaat
    35941 agtcgtgcgg tatagtccgc cagaataccc gaagcgccaa tgccaaaaat tgcgacttgc
    36001 cgtgcctgta ccaacaacgc taccgcttgc gcaagcgcgt ggcggttttc aggctctgac
    36061 aggacattac aggtatacaa atgcccttca agaacaaagt cgattgcggt gttaacatcg
    36121 ctcgtcaagt tactcaccgt cgtagacatc ttttcgctgg aactaagggc tggaccaagc
    36181 cactgttcca gagtgcgttt cagatcacgc agcccggcaa accccagggc ctgtatagcc
    36241 ctgataactg tggcatcgga agtgttcaac gtggccgcaa tttccatcgc cgtttgatcg
    36301 agtaccactt cacgatgctc attaatatag ctggcgacag ctcttaaccc aggcgttaac
    36361 tgactcgcgc gagtgcgaaa acgctcacca taaatatcaa cccgcgtttt gtctttctga
    36421 atcatttggc ctccggtaaa ggtcgccccg caatttgtga ttgtatctgc gcagccatca
    36481 gccccagaga tgcaaaggag ttagaaattg cctcttcacg agaaatcagc acataacgcc
    36541 cggaacgaca ggcagtcaga aactgacacc atcccggcat gactttttcc attgctgcca
    36601 gctcatcctg aggtttacca ccggtgtcac cgcgccaggt tgcaaaaaca aaatcggcat
    36661 ccagttcagg taaacgctcc gcgctcacat ccattcgccc accttcagga atgctctcaa
    36721 tcagcggcgg aaagcgaaaa ccagcatcgc gtaatacccg ccccagcgaa tgatagctat
    36781 gcatcacatt aatttttccc tgatttgcct gaatcaccga taccgttatt ttttggctgt
    36841 ccagcgtcgc cttcagcgca ttgatttgtg cctgatagcg gcgttcaagt atcgccagct
    36901 gcgactgcgt tcctgtcagt tctgccagct tgcgatagat ttctggcgcg ccccctttga
    36961 gatgatcaat gctcaccgtg ggcgcaattt tttccagtcg ttcaatgggt gtattacggg
    37021 tcggttctgt gatgatcagg tcaggctttg ccgccacaat cgcttcaata tcaatatcag
    37081 cggtgccaat aaaagcgatc gatgaattat caaagtcgac acctgtcaat aacgcgcctg
    37141 atcgaatgaa atggctaccg tcaggccgag ttcgcccgtg gctggcgaca ggaggtacac
    37201 cgagctcaat cagaggaata gtgatatcaa gatcgtgtaa agagacaatt ctctttggat
    37261 gagcaggaac agtgactttg cgccctaaat catcagtaaa gacctgaaca ggttctgtcg
    37321 cgcgggcaaa gaatgacaac agtatcagca gagaaaaaaa taaacgcata taactcccat
    37381 taaaaactgt tcctgcgctg ccagagcagt agcaaaaaga atggtccacc aatcaatgaa
    37441 ataatgattc cggcaggcag ttgcaaaggc aaaaaagcca aacgaccaat gctatccgcc
    37501 aggatgacta acaacgcccc cgatacggcg ctccccagca gtaatgtcgt ctgcccgcca
    37561 cgtagaataa aacgtgatat atggggtgct atcaatccga caaaaccgat gttaccaaca
    37621 cacgaaacgc agacggctgt caggattatt ggtgcggcga cacgtaacaa cgccagacga
    37681 gaactattca cccctaaccc ggttgcaacc tgatgaccaa gcagagcgat atccgccgtc
    37741 ctggcggtga acagcaataa aagagcggct ggcaacatcc aacaggctga aataccgact
    37801 aacatccagt tagcagcatg caggcttccc gataaccaca tcaatgcagt ttgtacatct
    37861 cgaacatctg cagtggtcat aaatacaccg ataccggcgg cgaaaaacca ggaaacacca
    37921 ataccaatca gaacaaatcg cgggcgggaa atttcgcgtg cacaaaaaat cacgattaaa
    37981 gccaccaaca agccaccagc aagaccagcc actggacgcc agaacattcc cagcattgga
    38041 aactggaaaa tcagccacag caccgccacg ctacatcctt ctttgacgcc gattaaacca
    38101 ggatcggcaa ggccgttacg cgcaatggat tgcatcgcgg cccccgccat acctaacatg
    38161 gctccgcata gcaatgccat gatgactctc ggcaagcgaa tatcctgcac aatataacgt
    38221 gcgtctgtgc ttacgttttc tggagcaaac aacgctcgtc ctacttccga tgccgggaca
    38281 aaaaaagaac catgcatcag accgaatatc agtattccaa ccgcaaacag aaacagccca
    38341 ccaagcaaca ccagattttt agggcgtacc agtgtcgaaa acttaccaac acgtagtgga
    38401 cgtagcccga cgttcattat ccgactcatt tgaacattct cgtcgccata atgacaaaaa
    38461 caggcgcacc aactaacgcc gtcatgacac cggtcgctaa ctcatggggc gtgaaaagcg
    38521 tccgagcaat aatatctgcc agtaataaca aaagcgcgcc gacacaagct gaaagaggga
    38581 gcagaacgcg caaatccgct gataccagac gcctgacaat ctgcggtact agcagaccga
    38641 caaaaccaat ggggccagca atcgatacgg ctgcgccaca gagcaacgcg atcgcaagca
    38701 gagtgaatgt tcgcgtgcgg agtaccgaga cgcccagccc ctgcgccatg cgatcaccca
    38761 gtgccaacat gttaagagag ggcgcaaggt aaatggctaa cacaaccccc cccagagaaa
    38821 accaggcgga cgtacccagc gtcgcccaat cctgccccgc taaatcacct gctagccatg
    38881 tccgcatcgc cagcagtgtt tgttcgtcaa gaatcaggat tgcagccgtt attgaggaca
    38941 cgaacgccga tagcgccacg ccgcataaag taactttcat tggcgttagc cctgaacgcc
    39001 ctgcggagga aagcagcaat atcaacagga acaataacgc gccgcccgta gatgccagca
    39061 gtgggcgtcc aaccggaaag gccagcccaa gcgcactggc agcaacgaca gcaagagcgg
    39121 ctccggcatt taagcctaaa atatgtggct cccccaatgg gttacgaatg acagcctgca
    39181 acaaagcacc agcaactcca agagaagcac cggtaagcaa tgctgccgct aaacgcggca
    39241 aacggagtct tacaattacg ttgtgatcga agttttgcgg ttcaaaatga agaaatgcct
    39301 gcaacaccgt ttgtggtgca ataaatcggg caccaatccc tggatatacc acgcatccca
    39361 caagcagtaa ggcgaggaaa attacagtaa ccagccctgt gcgcatcata ctttttctac
    39421 accacgaaac ggcatgaaga acggctttcc cgtcagagta tttattgatg cgtgaacatc
    39481 aacgtcaaag acagctttta ccagctcagg cgtgcaatgc tccccctcat tcaacacacg
    39541 caccactttc ccctggcgta aaaatatcaa cgtatcacca taattgacgg caaaattcag
    39601 atcatgcaac accacgacga ctgtacgccc gtgatgacgc gttaaatcat gtaataactc
    39661 aagaatttca acctgataac gtaaatcaag ccaggttgtg ggttcatcca gtaggatata
    39721 aggcgtcttt tgcgccaaca ccatcgcaat ccagcaacgc tggcgctgcc caccagagag
    39781 tttttcaaca ggtaaatgag caaactcctg cgtaccggtt aatttcaggg cctcttctac
    39841 cgcctgctcg tcggcgtcgc tccattgacg tatgaaattt tgccagggaa aacgcccacg
    39901 cgacaccagt tcataaacgg ttaagccctc cggcaataat ggcgactgcg gcaagatccc
    39961 aagtctacgg gataacgctt tcgttggttg ctcgtgaatc gctttaccat ccagcaatat
    40021 tgagccgccc atgggttgca agacacgtgc aatggtgctg agcaacgtcg actttccaca
    40081 gccgtttgcc ccaaccagaa cggtcatttt ttctgtggga acggagaaag aaacaccatc
    40141 aacaatgatt ttcttgtgat acccggcaga aacattgtcg aggatcagcc cctgcccttt
    40201 tgcgttatgt gccacgtgta cgccaactta atcaaagaaa aacaaaaata aaacttattc
    40261 tcttttgaga gtttgacgcc acaaaatgta gtagtcaaga agttaaaagc catacaaaaa
    40321 caaggtaaat aacaggtgaa atatttttaa agataaaaat aaaatcatta aatattaata
    40381 aattaccatc aattgaattg cattaacctc tcataaaaac acatgtagca attttttatt
    40441 atttctcatt tactactaca ttaccgcatg attgaatgat tctcaattac atatccgatg
    40501 gtaaaataac aaacggatag tgcttagtga gcaacaagaa acaattttca ctcacgacaa
    40561 tccgtgagtt tttgggataa cagactaaat ggctaagttc acaccttcat tctcaggaat
    40621 caaaggtcgg gcgctctttt cactgctctt tgcagcacca atgattcatg caaccgacac
    40681 tgcaacgacc aaagatggcg aaacaatcac tgttacagcg gatgcaaata ccgcaactga
    40741 ggcgaccgat ggttatcaac ctctgagcac ttccacggcg acattaaccg atatgccgat
    40801 gctggatatc ccgcaggtgg tcaatacggt tagcgatcag gttctggaaa atcagaatgc
    40861 aacgacgctg gatgaagcgc tttataacgt cagtaacgtg gtacagacca atacattagg
    40921 cggaactcag gacgcctttg tacgtcgtgg ttttggtgct aaccgggatg gctccatcat
    40981 gaccaacggc ctgcgaactg tacttcctcg cagtttcaac gccgccacag aacgtgtgga
    41041 agtgctaaaa ggtccggcct ccacgctgta tggcattctc gatcctggtg gattgattaa
    41101 cgtcgtgacc aagcgcccgg aaaaaacatt ccatggttct gtctcagcca cgtcctccag
    41161 ttttggcgga ggcactgggc aacttgatat cacaggtccc attgaaggca ctcagctggc
    41221 ataccgcctg acgggggaag tgcaggatga agattactgg cgaaacttcg gtaaagagcg
    41281 cagtacattt attgccccgt cactcacctg gtttggtgat aatgcaacag taaccatgct
    41341 ctattcccat cgggactata aaactccgtt cgatcgtgga acgattttcg accttacgac
    41401 gaaacagccc gtaaacgttg atcgaaaaat acgttttgac gaaccgttta atattacaga
    41461 tggtcagtcc gatctggcgc aactcaacgc agaatatcat ctcaatagcc agtggacagc
    41521 gcgctttgat tacagctaca gccaggataa atacagcgac aatcaggctc gcgttaccgc
    41581 gtatgatgca acgacaggaa cgctgacacg gcgtgttgat gcaactcagg gatctaccca
    41641 gcgtatgcat tctactcgtg cggatctgca agggaatgtt gatattgctg ggttctataa
    41701 tgagattctg ggtggggtgt catatgaata ttatgatctt ctgcgcacag atatgattcg
    41761 ctgtaaaaac gctaaagatt tcaatatcta caaccccgtt tatggcaata ccagcaaatg
    41821 cacaacggtt tcggcgtcgg acagtgatca gacgatcaaa caggagagct actcagctta
    41881 tgcacaggat gcgctctatc tgaccgataa ctggattgcc gtcgccggga tccgctatca
    41941 gtattacacg cagtatgcgg gtaaaggccg tccttttaat gtcaatactg acagccgcga
    42001 tgaacaatgg acgcccaaac tggggttagt ctacaaactg acgccatcgg tatccttatt
    42061 tgctaattat tcgcaaacat ttatgccgca atcgtcaatt gccagctaca tcggagatct
    42121 tccaccggaa tcatctaatg cttacgaagt cggggcaaaa ttcgagctgt tcgatggtat
    42181 aaccgcagat attgcgctgt ttgatatcca taaacgtaac gtgttgtata ccgaaagtat
    42241 tggtgatgaa accatcgcca aaacggcagg ccgcgttcgt tcaagagggg tagaagtcga
    42301 ccttgcggga gcattaactg aaaacattaa tatcattgcc agctacggct ataccgatgc
    42361 taaggttctg gaagatcctg attatgcagg gaaaccattg ccgaatgttc ctcgtcatac
    42421 cggttcgcta ttcctgacct atgacattca taacatgcca ggcaataaca cactgacgtt
    42481 tggcggtggt ggacatggcg taagccgtcg ttcggcaacc aatggggctg actattatct
    42541 gccaggctat ttcgttgccg atgccttcgc cgcatacaaa atgaaattgc agtatccggt
    42601 cacactgcaa ttaaacgtca aaaacctgtt tgataaaacg tattacacct cttccatcgc
    42661 cacaaataat ctggggaatc agattggcga tccgcgtgaa gtgcaattca cggtgaaaat
    42721 ggaattttga tcaaaaaagc ccggtataca cttgtatacc gggctatgtt ttattcttct
    42781 ggcgggagga ttatcagacc tcaatctccg ccatgtcgcc tttctcttgc aaccagttgc
    42841 ggcgatcttc cgagcgtttc ttcgccagca gcatatccat catcgcgtca gtacgctgat
    42901 cgtcttcatc atcgatagtc agctgcacca gacggcgagt gttcggatca agcgtggttt
    42961 cgcgcaattg catcgggttc atttccccca gacctttaaa acgctggacg ttcggcttgc
    43021 ctttcttgcg ttttaattgc tcaagtacgc cctctttctc ttcttccgtc agcgcgtaat
    43081 aaacctcttt cccgagatca atacggtaga gcggtggcag tgcgacgtaa acgtgaccgt
    43141 gtttcaccaa cgcgcggaag tgttttacga acaaagcgca gagcagtgta gcaatgtgca
    43201 aaccatcaga gtccgcatcc gcgaggatac agattttgcc ataacgaagc tggctcagat
    43261 cgtcgctgtc aggatcgata ccgatcgcta ccgaaatatc atgcacttcc tgcgaggcca
    43321 gcacttcgtc ggaagaaact tcccaggtgt taaggatctt acctttcagc ggcatgatcg
    43381 cctgatattc gcgatcgcgc gcctgcttgg cagatccgcc tgcggagtca ccttccacga
    43441 ggaacagttc ggtacggtta aggtcctgcg cggtacaatc agccagtttg ccaggcagcg
    43501 ccgggccgct ggtcagcttt ttacgcacca cttttttagc cgcacgcata cgacgctggg
    43561 cgctggaaat cgccatctct gccagcaact ccgccgcctg aacgttctgg ttcagccaca
    43621 ggatgaaggc atctttcacc acgcccgaaa cgaatgccgc acactgacgc gaagagagac
    43681 gctctttggt ctgcccggca aactgtggat cctgcatttt tactgacagc acataggcgc
    43741 agcgatccca gatatcttcc gccgacaact ttacaccgcg cggcaaaatg ttgcggtatt
    43801 cacagaactc acgcatcgcg tccagcaggc cctgacgcag gccattaaca tgggtaccgc
    43861 cctgcatcgt tgggatcagg ttgacgtagc tttcggtcag cagttcaccg ccttccggca
    43921 gccacagtag cgcccagtcc accgcttcag tatcgccagc gaaattaccg ataaacggtt
    43981 tttctggcag cgtcggtaaa ccgtttaccg cttccgccag gtaatcattc agaccgtcct
    44041 gatagcacca gcgctgttcg gtgttgttga tctcatcttt aaaggtaatc tcaacgccgg
    44101 ggcacagtac tgctttggct ttcagcacat gcgtcaggcg tgaaacagaa aaacgcgggc
    44161 tatcgaagaa ggtttcatcc ggccagaaat ggacgctggt gccggtattg cgtttaccgc
    44221 aattgccgac aacctgtaaa tcctgcacct tttcgccatt ttcaaaggcg atgttataaa
    44281 cctgaccatc gcggcgcacg ttaacttcta cgcgcttcga cagggcgtta accaccgaaa
    44341 tccccacgcc atgcaggccg ccagagaact ggtaattttt gttagagaat ttaccgcctg
    44401 catgcagacg gcaaagaatc agttcaaccg ccggtacacc ctcttccggg tgaatatcca
    44461 ccggcatccc gcgcccatcg tcaataactt ctaacgactg gtcagcatgt aaaataacgt
    44521 ccacgcgttt tgcgtgaccc gccagtgctt catccacact gttatcaatg acttcttgcc
    44581 ccaaatggtt agggcgagtg gtatcggtat acatccccgg acggcggcga accggctcaa
    44641 gcccggtgag tacctcaatg gcatcagcgt tataagtttg cgtcatggtt taagttagca
    44701 attcgagttg atcgtcagag atggtgcaga ccaagaaaat cgacgatcgg gttgaaataa
    44761 tcttcgaagc ccgtgaatgc gtggttgccg ccttctatga cagtctggcg gcaggaagcg
    44821 tagtacgcca ccgcctggcg gtaatccagc acttcatctc ccgtctgttg cagcagccag
    44881 atcaaatccg gcgcttccag cgggtcaatc tgcatgactt taagatcgta aatatggcgt
    44941 gactctagca catattgctg cccggtgtag gggttctcgt tctgaccgag atagtccgtc
    45001 agcagttcaa acgggcgcac cgccgggttt accaccactg cgggcagcat aaaacattgt
    45061 gacaaccagg tggcgtaata tccccccagt gacgaaccga caatacccag cgaatcgccg
    45121 ccatgttcca ggacaatgga ttccagcagc tctgccgcgt cggaaggata cggcggcaac
    45181 tgcggaatga tcatctcaac gtcagggtga tgttccgcca gccagttttt taacaagctc
    45241 gcttttgcag agcgcggcga gctgttgaaa ccgtgtaaat aaagaagcgt agacatcagt
    45301 agccttctga agcggtatca ggttggaaac gtgtgtccgc caggcgatgc acctcggtgg
    45361 tcagcgtgcc atcagcatgt aactcgagag tacgccagcc gggcgcgata gtatccagcg
    45421 taaagttgga acagtgcggc ttaaactgca cacaggtcga cggtgttgcc agcaggcggc
    45481 gaccgttcca gtcgagatcc agctcctgat gaatatgacc gcacagcaag tatttgacgt
    45541 gcggaaactt cgccagcacg gtatccagtt cgcccgcgtt acgcaggctg tgttgatcaa
    45601 gccaactaca acccgcaggt agcggatgat gatgcagcag cagcaacgta tggcgctctg
    45661 gcgcatcagc cagtttacgt tccagccact caagctgaaa ctcgcttagc tcaccgtgcg
    45721 gcacgccaaa cacctggcta tccagcaaca ggatttgcca ttgctcacca ataaacaccc
    45781 gcttcgccgg ggagataccc gcatcctgta acgcgctgta catcgcgggc tggaaatcgt
    45841 ggttgcccgg cagccagacg cagggcgcac gaaaacttgc gatgccttca gcgaaatgct
    45901 gataggccgc agaggattga tcctgcgcta aatcacctgt cgcgacaatc aggtcgaatt
    45961 cgtgctggtg tggacgaatc gcctccagca ccgcctggta gctttcccag gtgtttaccc
    46021 ctaagagggc ttcgtgcttt tgtgcaaaca ggtgagtatc ggtaatttgt aaaatcctga
    46081 ctctggcctc accagccaga ggaagggtta acaggctttc caaatggtgt ccttaggttt
    46141 cacgacgcta ataaaccgga atcgccatcg ctccatgtgc taaacagtat cgcaaccagt
    46201 ccgctaaaaa ctggttaatt tgatgctttt cgtcgcgttg atgcaacttt ttattaggat
    46261 aatcataccg cgctttgaag cgaaaaatct gctggcttga acacacttca gccaccatcg
    46321 cgtcatgata cagacgcacc gtcattgacg gaaggctcca gtaactgatc gcgggcgcag
    46381 tctgttctat tgtcaccagg gtagtgtatc gggtcgattc cacaatcgtc agccgatatt
    46441 gtgcgtttgc cacctgatag cttacagttt cgccgggtgc gtcattgcgc ggtaacaaac
    46501 ggcgcaattg tgaaaagttc atctcgcaca ggcgcatcat ttcaggaaag tcaggtgtgt
    46561 aacgcttcat ttatgcccac tcatttttta acgcttgatg atgcagctgc agccattgca
    46621 aagcgatgac cgacgctgcg ttgtcgattt tcccctcttc tacccactgg tatgcctgtt
    46681 cccggcttac cacatgaacg cgaatatctt cgttttcatc agccagaccg tgaataccgc
    46741 ttgcggtcgt ggcgtccact tcgcccacca taattgacga acgctcactg gtgccccccg
    46801 ggcttgccag gaaacttaac accggtttgg tccgtttgac tatcagcccc gcctcttcaa
    46861 tcgcttcgcg acgggcaaca tcttccacac tttcaccctc ttcaatcatt ccggcaacca
    46921 tctccagtag ccaaggggtt tcgctagtgt cgtatgcggc aattcgaatc tgctcaatca
    46981 gcacaacttc atcacgcact gggtcaaagg gtagcaagac tgcggcgtga ccgcgctcaa
    47041 aaatttcccg ccgtacctca tgactcattt gcccgttgaa tagacgatga cgaaatctat
    47101 aaagatctaa tgaaaaaaag ccgcgataaa gtgtttctcg tgcaataatt tctacatcgt
    47161 ttttgccaaa tgtaacgggc aggttgtctg gcttaagcat tgttaatgtc ctggcactaa
    47221 tagtgaatta aatgtgaatt tcagcgacgt ttgactgccg tttgagcagt catgtgttaa
    47281 attgaggcac attaacgccc tatggcacgt aacgccaacc ttttgcggta gcggcttctg
    47341 ctagaatccg caataatttt acagtttgat cgcgctaaat actgcttcac cacaaggaat
    47401 gcaaatgaag aaattgctcc ccattcttat cggcctgagc ctttctgggt tcagttcgtt
    47461 gagccaggcc gagaacctga tgcaagttta tcagcaagca cgccttagta acccggaatt
    47521 gcgtaagtct gccgccgatc gtgatgctgc ctttgaaaaa attaatgaag cgcgcagtcc
    47581 attactgcca cagctaggtt taggtgcaga ttacacctat agcaacggct accgcgacgc
    47641 gaacggcatc aactctaacg cgaccagtgc gtccctgcag ttaactcaat ccatttttga
    47701 tatgtcgaaa tggcgtgcgt taacgctgca ggaaaaagca gcagggattc aggacgtcac
    47761 gtatcagacc gatcagcaaa ccttgatcct caacaccgcg accgcttatt tcaacgtgtt
    47821 gaatgctatt gacgttcttt cctatacaca ggcgcaaaaa gaagcgatct accgtcaatt
    47881 agatcaaacc acccaacgtt ttaacgtggg cctggtagcg atcaccgacg tgcagaacgc
    47941 ccgcgcgcag tacgataccg tgctggcgaa cgaagtgacc gcacgtaata accttgataa
    48001 cgcggtagag cagctgcgcc agatcaccgg taactactat ccggaactgg cggcgctgaa
    48061 tgtcgaaaac tttaaaaccg acaaaccaca gccggttaac gcgctgctga aagaagccga
    48121 aaaacgcaac ctgtcgctgt tacaggcacg cttgagccag gacctggcgc gcgagcaaat
    48181 tcgccaggcg caggatggtc acttaccgac tctggattta acggcttcta gcgggatttc
    48241 tgacacctct tatagcggtt cgaaaacccg tggtgccgct ggtacccagt atgacgatag
    48301 caatatgggc cagaacaaag ttggcctgag cttctcgctg ccgatttatc agggcggaat
    48361 ggttaactcg caggtgaaac aggcacagta caactttgtt ggtgccagcg agcaactgga
    48421 aagcgcgcat cgtagcgtcg tgcagaccgt acgttcctct ttcaacaaca ttaatgcttc
    48481 tatcagtagt attaacgcct acaaacaagc cgtagtttcc gctcaaagct cattagacgc
    48541 gatggaagcg ggctactcgg tcggtacgcg taccattgtt gatgtgttgg atgcgaccac
    48601 cacgctgtac aacgccaagc aagagctggc gaatgcgcgt tataactacc tgattaatca
    48661 gctgaatatt aagtcagccc tgggtacgtt gaacgagcag gatctgctgg cactgaacaa
    48721 tgcgctgagc aaaccggttt ccactaatcc ggaaaacgtt gccccgcaaa cgccggaaca
    48781 gaatgctatt gctgatggtt atgcgcctga tagcccggca cccgtcgttc agcaaacatc
    48841 cgcacgcact accaccagta acggtcataa ccctttccgt aactgatgac gacgacgggg
    48901 cttcggcccc gtctgaacgt aaggcaacgt aaagatacgg gttatctgcc gcattcttcc
    48961 cccttctcgc ttcaatttcg accagccatc ctctattctg atgggtattt accacaggtc
    49021 ccggaagaca aaaatgaaac ggacaaaatc catacgccac gcatcgttcc gcaaaaactg
    49081 gagcgcacgc catctgacac cagtcgctct cgcggttgcc actgttttta tgctggctgg
    49141 ctgtgaaaag agtgatgaaa cagtgtctct ctatcaaaat gctgacgact gttcagctgc
    49201 aaacccaggc aaaagcgccg aatgtaccac cgcgtacaac aatgcgctga aagaagccga
    49261 acgtactgcg ccgaaatacg ccacccgtga agactgtgtt gctgaatttg gtgaaggtca
    49321 gtgccagcag gcaccagccc aggctggcat ggcaccagaa aaccaggcgc aggcccagca
    49381 atccagcggg agtttctgga tgccgctgat ggccggttac atgatgggtc gtctgatggg
    49441 cggcggtgcg ggatttgcac agcagccgct gttctcctcg aaaaacccag ccagcccggc
    49501 ttacggtaaa tataccgacg cgacgggtaa aaactatggc gcagcccagc caggccgcac
    49561 catgaccgta ccgaagacag ccatggcacc aaaaccggcg accaccacta ccgttacccg
    49621 tggcggtttt ggtgaatctg ttgccaaaca aagcactatg cagcgtagcg caaccggtac
    49681 ctcttctcgt tcaatgggtg gctgataccg atggaaagag tcagtattac cgagcgcccg
    49741 gactggcgtg agaaagccca cgaatacggt ttcaattttc acaccatgta cggcgagccg
    49801 tactggtgtg aagatgctta ctacaagttg accctcgccc aggttgaaaa gctggaagaa
    49861 gtcaccgccg aactgcacca gatgtgcctg aaagtggtgg aaaaagtgat cgccagcgat
    49921 gagctgatga ccaaattccg cattccaaaa cacacctgga gttttgtgcg ccagtcatgg
    49981 ctgacgcacc agccatcgct ttattcgcgt cttgatctgg cgtgggatgg cactggcgaa
    50041 cctaaacttc tggaaaataa cgccgatacg ccaacgtcac tatacgaggc ggcgttcttt
    50101 cagtggatct ggctggaaga tcagcttaac gccggtaact tgccggaggg cagcgaccag
    50161 tttaacagtc tgcaagaaaa actgatcgat cgcttcgttg agctgcgtga acagtatggc
    50221 ttccagttgc tgcatctcac ctgctgtcgc gacacggtgg aagatcgcgg taccattcag
    50281 tatttgcagg actgcgcgac ggaagctgaa atcgctaccg agttcctcta cattgacgat
    50341 atcgggttag gtgaaaaagg tcagttcacg gatttacagg atcaggtaat ttccaacctg
    50401 ttcaagctgt atccgtggga atttatgttg cgtgagatgt tttccaccaa gctggaggat
    50461 gcaggcgtac gctggctgga accggcgtgg aagagcatta tctccaacaa ggcacttcta
    50521 ccgctactgt gggagatgtt cccgaatcac ccgaacctgc tgcccgctta ttttgcggaa
    50581 gatgatcatc cgcaaatgga aaaatatgtg gttaaaccga tcttctcccg tgaaggcgca
    50641 aacgtgtcga tcattgagaa cggcaaaacc attgaagcag cggaaggtcc gtatggcgaa
    50701 gaagggatga ttgttcagca attccacccg ttaccgaaat tcggcgacag ctatatgctg
    50761 attggtagct ggctggtgaa cgatcaaccc gccggaattg gcattcgtga agaccgtgca
    50821 ttgatcaccc aggatatgtc tcggttttat ccacatattt ttgttgaata atcaatgatg
    50881 ccggatggca ttcgccatcc ggtaattgtt aacctatctg caccgacagc atacttaaac
    50941 tgcccatttc tataccatcg accggaatgg taattggctc ctgcccatcc cacgcaccta
    51001 agacatacag caacggcaga tagtgctctg gcgttgggtt cgataacgcg ccaccttcat
    51061 ggtcgagata attcaccaga ggatgctgct ctaccggccc ttgccacgtc agattcgctt
    51121 tcacatactc attaaacgac atcgcccaag gatacggtga actatcaccg tgccacttca
    51181 ctgtacgcag gttatgcacc acgttgccgc tggcgaccaa cattattcct tcatcacgca
    51241 gcgccgcgag ttttcgcccc atttcgaaat gccaggcggc aggtttgcta ctgtcgatac
    51301 tcaactgcac catcgggata tccgcgtcag gatacatctt aatcagcacg ccccaggaac
    51361 cgtggtcaaa gccccaggct tctttatcca gcgtcaccgg gatcggcgct aacagctcaa
    51421 ccagacgctg tgccagcgca ggcgaacccg gagcaggata atgcgtatcg tacagcgcct
    51481 gcgggaagcc accaaagtca tgaatcgtgg gcggcgtctc catcgcggtc actcctgttc
    51541 cacgggtaaa ccagtgagcc gaaactacca caatcgcttg cgggcgtggc aatgtcatcc
    51601 ccaacgtctg ccagctgcgg gtatacaaat tatcttccag cacgttcatc ggactaccgt
    51661 gacctaaaaa caatgctggc atacgtgttg aagacatgat gatatcctta actaaaggtg
    51721 tcattttgat atcctcacga tacgcttgtt cggcggagta agaacccgga taacaatgat
    51781 gatgatcatc agttattttg acgatctgcc tgaaggtgaa gatttataag gagttgtcga
    51841 tgtcagtacc tctcattctg accatactgg cgggggcagc cacgtttatt ggcgcgtttc
    51901 tcggcgttct cgggcaaaaa ccctcgaacc gcttactggc gttttcgcta ggatttgcgg
    51961 cggggatcat gttgctcatc tcattaatgg aaatgcttcc tgccgcacta gcagctgaag
    52021 gaatgtcgcc tgtgttgggt tatggaatgt ttatcttcgg tctgcttggc tattttggcc
    52081 tggaccgcat gttgccgcat gcgcatccgc aggatttaat gcaaaaatcg gtgcagccgt
    52141 tgccaaaatc gatcaagcgc acagccattc tgctcactct cggcatcagt ctgcataact
    52201 tcccggaagg gattgccacc tttgtcacgg cgagcagcaa cctggagctg ggatttggca
    52261 tcgcactggc cgtcgcgttg cacaatattc ctgaaggtct ggcggttgca ggcccggttt
    52321 atgcagcaac gggttctaaa cgcacggcaa ttctgtgggc ggggatttct ggactggcag
    52381 aaattcttgg tggtgtgctg gcgtggttaa tcctcggtag catgatttcc ccggtggtca
    52441 tggcagcaat catggcggcg gtcgcaggaa ttatggtggc gctctcggtt gatgaattaa
    52501 tgccgctcgc caaagagatt gaccctaata ataaccccag ctatggcgta ctatgtggaa
    52561 tgtcagtgat gggattcagt ttggtgctgc tacaaacggc gggaattggt taaaagcaac
    52621 atcagaagtt gtccagcgca gggacaactt ctgctagttt cactaaagcc ttattctacc
    52681 ttttcggctg tctcttctct cgtactgttt aagtatttgt tccgcgcttc aagcagtgcg
    52741 tctctgtcct ggcgaaatgc ccgacaataa cgtttcaaat gcaggaaata acccaccaca
    52801 accagaacaa tcaatacaat ccagtaccag gcgataaaca tccttttacc cttaatattt
    52861 aacaatacta aacatttagc gcataaattt cacatatcct ttttcggata tattcatatg
    52921 gtcgcataat aacggaacaa ttatatgatt ttgatcataa tgaaaaagaa tgcctgatat
    52981 taatgaaatt ttctcataga aagagaagcg gaattaacat cttgcatagt agggaaaaat
    53041 aaaaaccgaa gcctgctaca acttcggttt cttgattaag gcagtaaatt aagcagcggt
    53101 tttcagctgg ctttacgctc atgtgcctga cggtatgcca ccaggtcttc aatagtcacg
    53161 agcgccatgt tgtgtttatt ggcaaactca atgcactctg gtgcacgcgc catcgtgcca
    53221 tcgtcattag tcagctcaca cagcacacca gccggtttga agcctgccag cgtcattaga
    53281 tcaatagttg cttcagtatg accgccacgc gtcagtacac caccagcctg agcgcggagt
    53341 gggaaaacgt ggcccgggcg attcagatca gaaggttttg cgccatcggc aatcgctgcg
    53401 cgaacggtcg taatacggtc agcggcagaa acaccggtag tcacaccttc agccgcttca
    53461 atggtcacgg taaaaccggt gccataggcg ctggtgttat tttctaccat cattggcaga
    53521 tcgagttgtt tacggcgatc ttcagtaatg cacaggcaaa caataccact accgtggcga
    53581 atggtcagcg ccatctgctc aacagtcatg gtttctgccg ggaagatcat atcaccttcg
    53641 ttttcacggt cttcatcatc aagcaccatt acaccgcgtc cttcacgcag cgcagccagt
    53701 gcattttcaa cacgttcgaa aggcgtacca aaagaggaaa gtagcgtctg attcatggta
    53761 aaaaaacctc actaaaatta tggttaccag aatcagggca gtcttaggag tggcggcata
    53821 tagccaaaat aacgtgagcg ggtccatgcc cgacagaatc gttactctct cccatccgga
    53881 ctctaaccgt cggccccgga attacaccgg atctgctgtc ctttgagttc gcacccaaag
    53941 cgctcgcggg ctttcaactg agttgattta ccgccggtgg ggaatttcgc cccgccctga
    54001 gaataagcgg attcactata acgctaatga ttagcggcag caacgcatag cttcacataa
    54061 ttctggttta tgacttaccc ttatcgcact acaatggcac tcaacaccta tcagtacagg
    54121 gaaactacaa tgattgaccc gaaaaaaatt gagcaaatcg ctcgccaggt tcacgaatca
    54181 atgcctaaag gaatcaggga gttcggggaa gatgtggaga aaaaaatccg ccaaacccta
    54241 caagcgcagc tgacgcgtct cgatctggta agccgcgaag aattcgacgt tcagacgcaa
    54301 gtcctgttgc ggactcgtga aaaactggcg ttactggagc agcgcagcag tgaactggaa
    54361 gcgcgcaata attcggtagc agatcttcaa tctcctccgg ctatcccgcc tattgataaa
    54421 gcagaataaa gcaacacaaa aacgggccag acggcccgta atatcagtgt tctaattcaa
    54481 gttctctggt ttgcatcatc tgttggcaat accaggcata acgtgcgcaa aaccaggttc
    54541 tatgttcttc atccatgtta ccagtaacgg ccagaatatc gaccgggcga ccttctgcgt
    54601 gcattctggc aaaactacgc gctaggaaat cgaaattatt taaagaatta agactatgat
    54661 ccataagcac atcctccggt tatcgcatac cttttaaggc catatgcttt ttctgatttc
    54721 agtattcccc aatcagtgta gtgcctttaa acataacgtg cgtaaatata ttgtccccga
    54781 tcacactttt tagtgaaaaa taaaaaacca tattaaaaga ctttctcttc cagaacctct
    54841 ctaacatatg ttattattaa caaagacaaa tgtaagcaag gaaactatca caaagtaaat
    54901 attgcgtcac taaatggaca ttggagtgac atatgatttt attcgccgac tataacaccc
    54961 cttatctttt cgctatttcc tttgtactgt tgatcggcct tttagaaatt tttgcgctta
    55021 tctgcggtca catgctctcc ggtgctcttg atgcacatct tgaccattat gattccataa
    55081 ccacaggtca tattagccag gcacttcatt atctcaatat cggaagattg ccagctctcg
    55141 tcgttctttg cttactggct ggtttttttg gtcttattgg catcctgttg cagcatacct
    55201 gcatcatggt ctggcaatca ccactctcga acctgtttgt ggttcctgtc agtttactat
    55261 ttacgattat tgcggtgcat tataccggta aaatagttgc gccctggatt ccacgcgatc
    55321 atagctcagc tattacagaa gaagaatata ttggtagcat ggcgttaatt accggtcatc
    55381 aagccacttc aggtaatccc tgcgaaggaa aactcaccga tcattttggc caaatacatt
    55441 atttactact ggagcctgaa gaaggaaaaa ttttcactaa aggagacaaa gtgttaatta
    55501 tttgccgact ttctgcaacg cgatatttgg cggaaaacaa tccctagccc caaatattgt
    55561 aatttattaa tcaaaaggaa atttaataat ggatgatatt gttaattccg tgccctcctg
    55621 gatgtttacc gcgattattg ccgtatgcat tctgtttatt attggaatta ttttcgccag
    55681 gctctatcgt cgcgcttcgg cagagcaagc atttgtccgt accggcttag gggggcaaaa
    55741 agtggtaatg agcggtggcg caatcgtgat gccgatcttt catgaaataa tccccatcaa
    55801 tatgaatact ctgaagctgg aagtcagccg ctcaaccatt gatagcctga ttacgaaaga
    55861 tcgtatgcgc gtcgatgtag tagtcgcttt ctttgtgcgg gtaaaacctt cagtagaagg
    55921 gattgccacc gctgcccaga cgctggggca acgcaccctg tcgcctgaag acttacgtat
    55981 gttggttgaa gataaatttg tcgatgccct ccgtgcaaca gctgcgcaaa tgaccatgca
    56041 tgagttacag gatacccgcg agaacttcgt gcagggagta caaaataccg tagctgaaga
    56101 tctgtcgaaa aacggcctgg aactggaaag cgtttcactt accaacttta accagacctc
    56161 gaaagaacat ttcaatccta acaatgcctt tgacgccgaa ggtttaacca aactgactca
    56221 ggagacggag cgccgtcgcc gcgagcgtaa cgaagttgaa caggatgtag aagttgcggt
    56281 gcgtgagaaa aaccgtgatg cactttcgcg caagttggag attgaacaac aagaagcgtt
    56341 tatgacgctt gagcaggagc agcaggttaa aacccgtacc gctgagcaga atgcgaaaat
    56401 tgcggctttt gaagctgaac gtcgtcgtga agcagagcag acgcgaattc tggctgaacg
    56461 acagattcag gaaacagaaa tcgaccgcga acaggccgtc cgctcaagaa aagttgaagc
    56521 tgaacgtgaa gttcgcatta aagagatcga acagcagcag gtcaccgaaa tcgctaacca
    56581 gacgaaatcg atcgctattg ccgccaaatc ggaacaacag tcccaggcag aagcgcgtgc
    56641 taaccttgca ctcgcagaag cagtaagcgc ccaacaaaac gtagaaacca ctcgccagac
    56701 agccgaagcc gatcgtgcca agcaggtggc gctgattgcc gcagcacagg atgcagaaac
    56761 caaagcggtt gaactgaccg tgcgggcgaa agcagaaaaa gaagccgcag agatgcaggc
    56821 ggcggcgatc gttgagttag ccgaagctac gcgtaaaaaa ggcctggcgg aagcagaagc
    56881 acaacgtgcg ctgaacgatg ctatcaacgt actttctgac gaacaaacca gccttaaatt
    56941 caaactggcc ttgttgcagg cgctacctgc ggtaatagag aaatccgtgg aaccaatgaa
    57001 gtccattgac ggtatcaaga ttattcaggt cgatggtctg aatcgtggtg gtactgcggg
    57061 ggatgcaaac acgggtaatg tggggggcgg aaacctggcg gaacaagcat tatcagccgc
    57121 actctcttac cgcacacagg caccactgat tgactccttg ctcaatgaaa ttggcgtttc
    57181 aggcggctca ctggctgcat tgacttcact cttaacccca acaactcccg tcgccgaaaa
    57241 cgtagaataa attttcagct atgaacatcc ctggctcaaa aagccgggga ttttttatat
    57301 ctgcgttccg ctaaaaggtg caaatgatca ggctgttgca gcgttttgcg tgattgctcg
    57361 gggaaggcaa aactgcttca gggaaagcgt tgcgcggggt ccggcgctca tcaacaatcg
    57421 gggagtaagc aaagggctga aacgggaaag cccctcccga ggaaggggcc ttacagaagg
    57481 aaagggttat gatgaagctc gtcatcatac tgattgtgtt gttactcgta agtttcgcag
    57541 cttattaaca gccaatcaga ggggggagaa atcctccctc acccttgttc ctttacttta
    57601 ggttgaaaaa aaatcagcgt caagaaggtt tcattaacac atatccctgg cccaaaaagc
    57661 cggggatttt ttatatctgc gttccgctaa aaggtgcaaa tgctcaggac gttgcagcgt
    57721 tttgcgtgac cgctcgggga aggcaaaact gcttcaggga aagcgttgcg cggggtccgg
    57781 cgctcatcaa caatcgggga gtcagcaagg ggctgaaacg ggaaagcccc tcccgaggaa
    57841 ggggccttat agaaggaaag ggttatgatg aagctcgtca tcatactggt tgtgttgtta
    57901 ctgttaagtt tcccgactta ctaacaactc atcagagggg ggagaaatcc tcccttaccc
    57961 ttgttccttt actctaggtt gaaaagacaa cagcgtcaat aggcctgcca tgtacgaagc
    58021 gagatctgtg aaccgctttc cggttagcct tttttatcct gttggatctt cttgataatg
    58081 ttagtggtcg agcaaccgtc ttcaaagttg agcaccaaca cttcgccgcc gttggcccag
    58141 acttctttac tcccggcgat ctcttccggt ttgtagtcac cgcctttcac cagcaggtct
    58201 ggcaggatcc cggcgatcag acgctgcggc gtgtcttctt caaacgacac tacccagtcg
    58261 accgcttcca gtgcgcccag cacaatcata cgctgttcga gtgggtttac cgggcgggaa
    58321 tcccctttca gccgtttggt ggaggcatcg ctgttgacgg caacaatcaa gcggtcaccc
    58381 agcttgcggg catttgccag ataagagacg tgcccggcat gtaggatatc gaagacaccg
    58441 ttggtcatta ccactttttc accacgttta cgcgctgcca ctacagccag cttcagttcc
    58501 tcttcggtca tcacgccaaa gccggtatct gcgcgtccac gtacagcgtt ttccagctcg
    58561 atcggcgaaa ccgtggacgt ccccagtttg ccgaccacca caccagccgc cgcattggca
    58621 aagaagcagg cttcttccag cgaattaccc gctgccagcg ttgccgccag gacgccaatc
    58681 accgtgtcgc ccgcaccggt aacgtcatac acttcctgcg cctgagttgg catatgcagc
    58741 ggcgctttac ccggttgcag cagcgacata ccctgttcgg aacgggtcac taacagagcc
    58801 gagagttcgt aatcggcaat cagtttcatg ccgcgctcaa caatctcttc ttcggtctta
    58861 catttaccga caacagcttc aaattccgag agattcggcg ttaacagcgt agcgccgcgg
    58921 tagcgctcaa aatcggtacc ttttggatca atcagcaccg gaacacccgc tttacgcgcc
    58981 agttggatca tctgctgtac gcttgccagc gcacctttgg cgtagtcaga aagcaccagc
    59041 gcgccaatcg aactcagcgc ctgattaatc cgctcgtgca gcggctgcgg atcaacacct
    59101 tcgaaacctt cttcaaaatc cagacggatc agctgttggt tgcgggaaag tacccgtaat
    59161 ttggtaatgg tcggatgcgt cggtacagaa acgaagtcgc atttgacgtt gacgtcggcc
    59221 agagatttac tcagcgcgcg cgctgcatcg tcaatgcccg tcaacccgac caggcgtgca
    59281 ttagcaccga gagaagcgat attcatcgcc acgttagccg cgccgcccgg acgttcttcg
    59341 atggtattca ctttaaccac gggcaccggc gcttccggcg agatacgact ggaggggccg
    59401 taccagtaac gatccagcat cacatcacca accaccatca ctcctgcacg ttcaaactct
    59461 ggcagcgtta ctttcattcc tgtctcctga gagattcaaa atttgcgcgc gataatacca
    59521 tacttcattc ttccaccagc cacttctgcc agcttgcccg caccagttca cgctctgcgg
    59581 tgaagcaatc ctccggcaca tggcccggca attcctgtaa tgccagatga tgaagttcat
    59641 cgcgaagcgt agtgtaagca cgggtcagcg ccatcgcttc ctgctcttcc ataatgtcgt
    59701 tttgcgccag tagttccaga atgcgcacgt tgtctgacca gcgcgttaac ttcggttttt
    59761 catgagcgta gcgcaacacc agatattggg taataaattc gatatcggta attccccctt
    59821 catcagcttt gatatcaaag cgatcgcgat gtttattgcc gagatgagcg cgcattttct
    59881 cgcgcatttc ccgcacttcc gtttgcagag ttttaccttc acgcggcagc gtcataatct
    59941 cgcgacgcac tgcgtcaaag tgcgcggtga gctgcggatc gccgtacact acacgcgcac
    60001 gcaccagcgc ctgatgctcc cacgtccagg cctcgttttt ctgataatcg gcaaatgctt
    60061 ctgtggaggt aaccagcatt cccgccgccc cggacgggcg cagccgcgca tccacttcgt
    60121 acaaaatgcc ggaagaggtt cgcgtgctga acaggtgcat aatgcgttgc gccagacgca
    60181 gataaaactg tcgtccgtcg atttcccgct cgccgtcagt catcgcatcc attgggcaat
    60241 catggaggaa gatcaggtca agatcggaac tgtagcctaa ctcccagccg cccagcttgc
    60301 cgtagccgac cactgcaaaa ccacgccctt cgcgttcgtt caggtgattc ggcttaccgt
    60361 agcgggcaac catttgaacc cacgcctgct gaacgacggc atctatcatg gcttccgcca
    60421 gccaggttaa gtgatcgctc actttcatca ccggtagcgt accggcgata tccgctgcgg
    60481 cgatgcgtaa cagctgcgcc tgtttgaact gacgcagcgc ctcaagctgt tgctcttcgt
    60541 catcttccgg cacgcgcagc aaatactggc gcaactcatc gcggtaggca tcggtcgccg
    60601 tcggctggta aagggtgttt ggatcgagca attcatccag caataatgga taacgcgcca
    60661 gctggctggc aatcatcggc gacgctgcac acagagaaat caaatgttta agcgccgcgg
    60721 ggaattcact gagcaattct aaataggtgg tgcgggtaac aatccccgcc agcaaggcgg
    60781 taatgcgcaa cagcgtaacg gcagcgtctt cacgcgcaca gacatcactt agcagatgcg
    60841 gcatcagatg gtcgagcacc tgacgcccgc gtggcccgat tgtgcgctta tccagctctt
    60901 tgcggaaatc ggcaatcagc gtcagcacct gtttgcgatc gtcctcgcta agatgcgcca
    60961 gcactggtgt agtgtcatct tcctgcaacg catcctgcca cagctcgcgc cactgttccg
    61021 acagcgactc ttcctgagtt tcactttcgt cgtcgccaat caattcatta aacacccggc
    61081 gcacattggt catatgtgcg gtcagcgccc cggtcaattg cggccagtca gcaaagtcca
    61141 tcgcccacgc cagccgcgca cggttaagct catcagaagg aagcgtctgg gtttgttcgt
    61201 cgttaatgct ttgcagcagg ttttccagac gccgcaggaa cagatacgcc actcgcaatt
    61261 gttcagcatc gttttcagaa agcagatgca gcgcggcaat gacgctgagc gttggcagta
    61321 aagagcgcga ctgcagcgac ggttcgcgcc cgccgcgaat gagctggaac acctgaacga
    61381 taaattcaat ttcgcgaatg ccgcctgcgc cgagtttgat attgtcggtc aaaccacgtc
    61441 gacgcacttc acgggcaatc attcctttca tgttgcgcag cgactgaatc acgctgaaat
    61501 cgatgtaacg acggaaaaca aacgggcgca gcatcgcacg caactcgtta gcatagacgc
    61561 cttcgctatc gcccataatc cgcgccttga ccatcgcgta acgctcccag tcgcgcccct
    61621 gctcctggta ataatcttcc aacgcggcaa agctcagcac cagcgggcca ctttcgccaa
    61681 acggacgcag ccgcatatcc acgcgataga cgaagccatc ctgcgttggt tgatccagca
    61741 ctttaatcag ccgctgcccc atgcgggtaa aaaactgcgc gttatccagt tcccggcgtc
    61801 caccctgcgt acaaccatgt tccggccagg caaaaatcag atcgatatca gaggagaaat
    61861 tcagttcccc gccgcccagc ttacccatgc ctaaaatcag cagcggttgc gcttcgccct
    61921 gcgcattgca cggcgttccc cactcgcggc agcaggcgtc atatagccag tcacgcgccg
    61981 caacaatcag cgtctccgcc agatagctga gctgctgcaa tatgctctct tcagtaacca
    62041 gtgccagcgt ttgcgcccag gcgatacgta ccatgatgcg ccgacggaac agccgcagct
    62101 cgcgcattaa cccggcttcg tcactcacat taatgagcgc ctcctgcaac catgcaacgt
    62161 aatgctgcca ttcgtcggcc tgcggcggtt ggctttccag ttccgtcagc cactctggat
    62221 gcgcacttat gctgtcctgc acaaaatcac tgaaagtaag tactgacttc gcctgtgcac
    62281 taagtgattc ctcggctaaa ggctctggca gccgctcaac aatggtctgc cagtactgct
    62341 gtaacggtga agagagcggc ttcataagcg attttatcct tgcctgatta acgttttccg
    62401 ctgtgcaacc agaacggttc ctgattgttt gcctcattac ggaaatgttc aatttcgatg
    62461 cgttgcccgg tcgcaatagc gtgatgcagc ccctgccagt tctccagcca ggcttgcgcg
    62521 acgacaggat catagtaacc cgccagcaac agtattgagt caatatcacg cgtcaggcgt
    62581 ggcaactggt cacggtagcg atcgcctaac ggctggcaga aaacgctttt cagttcagcg
    62641 gcatggcggg aaagatggat atcggcaaag cgtttgaagg agtcgctgat tttgccctgc
    62701 gctttggcat ctaaaaatgg ctgccatgct ttgcttacca accattcggt caacgccagc
    62761 ttcgccattg ccgtttcggt agagtagacc gccgtcacgg cagaaaccgc agaagcaatg
    62821 gtcgcttcgc attgggtcag caaatcacgt aagtgagtgc tcgctttacg cggcacaata
    62881 ccaccgaaca gcatcagcgt atgacggacc aagctaatgg ctgccagcac ctgttctttc
    62941 gccgcatcgt tgccgcgtac ccacagttct tcatgatact gccattgcgc taacgccagc
    63001 tcgagcgccg cttccagccc ctgttccaca tcggcttttg ccgcaacatg caaaatggtg
    63061 gtcggtttga tttcacgcgc cggattgccc tgcgccagat gatagccacg cgccgctttg
    63121 ctcaggctgc cctggcgtaa tccggtttgc gataccagtt ggttcgccag tttcagcacc
    63181 gcgcgcgtgt cgccgctaag cagctccagt tccagctcac agataggttc agcaaattcc
    63241 cccgctttca cctcaccctg atcgagagcg atttcaattc ggctgccatc gaccgccacc
    63301 agccattttt cgcgataaaa atcggtgctg aacagcggtt gtacgcggga agcgagatcg
    63361 gcgggcaatt cgccgttcgg ccagacttcc gtcggtaact gcgccaggtc gagcgtcggt
    63421 tcgctcaacg ccacgttata ttccgggcgc tggtgtaagc cgcctgtcac tcttcctgca
    63481 actttcatgg tcatctcata gcgaccgttt tcgccacgaa tacgtaagcc catatcgtgc
    63541 ccacgcagcc agttatccgg cgtttcgtag taaatattca gtaactgcac ggggtcatgg
    63601 tgctcgccgc ccagcgtatt gagatggtca cgcaacgcct caacggcgct gtgattaaca
    63661 ataaacttta attcgatttc ctgagccatg gcctttactt atcggttatg tcacataagt
    63721 gacgatgaac ggcgaactta atgcgatctt ttttgtcagt agatagtatt ttgcgccaaa
    63781 ttgccatgca acgggcaatt tgacgggcgt aaaagtttga agcagtggca agacctgaca
    63841 cagaggattc cgattgatgt cgaatgcttt gcgtagcgac actgatgcca ctactatcgt
    63901 tccactttcc atgacaataa cgacagcctg atgccaaaat tacgcctgat cggattaact
    63961 ttactcgcac ttagcgcgac tgccgtctca cacgctgaag aaacgcgcta tgtttctgac
    64021 gaactgaata cctgggtccg tagcggtccg ggagatcatt atcgcctcgt gggcacggtt
    64081 aacgccggcg aggaagtgac cttattacaa actgacgcca acaccaatta tgcccaggtg
    64141 aaagacagct ctggccgtac cgcctggatc ccgttgaaac aacttagcac tgagccaagc
    64201 ctgcgctccc gtgtgccaga tctggaaaat caggtcaaaa ccctgaccga taaactcacc
    64261 aatatcgata acacctggaa tcagcgcacc gcagaaatgc agcaaaaagt ggcgcagagc
    64321 gacagcgtga tcaacgggtt aaaagaagaa aatcagaaac tgaaaaacga gctgattgtc
    64381 gcgcagaaaa aggtcgatgc cgccagcgtg cagctggatg acaaacagcg caccatcatc
    64441 atgcaatggt ttatgtatgg cggcggcgtg ctggggcttg gcttgctgct cggtctggta
    64501 ctgccgcacc tgatcccaag ccgcaaacgc aaagatcgct ggatgaacta aatcgccttc
    64561 ttcgttgcac tgtcttatta ttaggaattg tgtatttgac gaagggatga tggcgtgaag
    64621 atttatctgg tcggtggtgc tgttcgggat gcattgttag ggctaccggt caaagacaga
    64681 gattgggtag tggtcggcag tacgccacag gagatgctcg acgcgggcta ccagcaggta
    64741 ggccgcgatt ttcctgtgtt tctgcatccg caaacgcatg aagagtatgc gctggcacgt
    64801 accgaacgga aatccggctc cggttacacc ggttttacct gctatgccgc accggatgtc
    64861 acgctggaag atgatcttaa gcgtcgcgat ctgaccatta atgcgctggc ccaggacgat
    64921 aacggcgaga ttatcgaccc gtacaacggt ctgggcgatc tgcaaaatcg tctgttgcgc
    64981 catgtttccc ccgcttttgg cgaagatccg ttacgcgtac tgcgcgtggc gcgttttgct
    65041 gcgcgttatg cccacctcgg ttttcgtatt gccgatgaaa ctctgacgct gatgcgcgag
    65101 atgacccatg cgggtgaact ggaacacctg acgcctgaac gggtatggaa agagacagaa
    65161 aacgccctca ccacccgcaa tccacaggtg ttcttccagg tactgcgcga ttgcggcgca
    65221 ctgcgcgttt tattcccgga aattgacgcc ctgtttggtg ttccggcccc tgccaggtgg
    65281 catccggaaa tcgacacggg tattcatacc ttaatgacgc tctcaatggc ggcgatgctg
    65341 agtccgcagg tcgatgtccg tttcacaact ttatgccacg atctcggtaa aggactgacg
    65401 ccgccagaac tctggccgcg tcatcatggt catggcccgg cgggtgttaa gttagtggaa
    65461 caactatgcc agcgtctgcg cgtgccaaat gaaattcgcg atttagccag actggtggct
    65521 gagtttcacg atctcatcca caccttccca atgctgaacc caaaaaccat cgtcaaatta
    65581 tttgattcca tcgatgcctg gcgtaaaccg cagcgtgtcg agcaactggc gctgaccagc
    65641 gaggctgacg tgcgcggcag aaccggtttt gaatcagcgg actacccgca aggccgctgg
    65701 ttgcgcgaag cctgggaagt ggcgcagtca gtgccgacaa aagccgtcgt tgaagcggga
    65761 tttaaaggtg tggagattcg cgaggagctg acccgacggc ggattgcggc ggtagccagc
    65821 tggaaggaac aacgttgccc aaagcctgag tgagtattgg gcattttgcc ggatgcggcg
    65881 taaacgcctt atccggctac atgtcaacga cagttgtagg cctgataaga cgcgcaagcg
    65941 tcgcatcaag cgttgattgc cggacgcggc gtgaacgcct tattcggcct acgcaatcgt
    66001 ggacaacgcc aggcatccga cactattcct caattaaaag aacacgacat acaccgcagc
    66061 cgccacaata aagcgataaa tggcgaacgg gataaacgaa atgcgcttaa tcaattgcag
    66121 gaaggtttta atcgctatca gcgccaccac aaaagcggtg ataaacccaa cggcaaacat
    66181 cgggatatcg ccgcttgtca ggaagcccca gcttttgtag agatcgagcg ccgttgcgcc
    66241 catcatcatc ggcaccgcca gcaggaacga aaactcggaa gcagcgtaac ggctcacccc
    66301 catcagcatc ccacctgaaa tggtcgcccc ggaacgggag aaacccggcc acagcgccag
    66361 acactggaaa caaccaatca taaacgcctg acgataggtc atatcatcaa gacccggcgc
    66421 acgcggctct ttcggcttca ggcattcggc ggcaatcagc aacaaaccgc caacgaccag
    66481 cgcatacatc acatttatcg ggttaaacaa tgacttaatc gtgtcgtgga acaacagccc
    66541 cagtaccacc gccggaatca tccccagcaa aatgtggatc agcgttaaac gacctttgct
    66601 ttctccttcg tgctgcaacg ggcggccaaa gtggatgcca atcaggccaa acagacgccg
    66661 ccagaacatc actactaccg ccagaattga tcctaactgg atcacaactt caaaggtttt
    66721 cgccgtgtcg ccctcaaacc ccaacaagtg accgacaata atcatatggc ccgtgctgga
    66781 taccggcaga aattctgtca atccttcgac cacacccaat attgccgcta tcagcagcga
    66841 gtgcatatcg ctcatcaata aacccctaaa ttataaaaat gtaacgcttg tccgaactac
    66901 tgcagtatga ccaggttata accgtttggt ttaacagctg taaaattaat tattttcttt
    66961 cagattattg ccacgctcaa tgattacgcc aacattcgcc gcccgcgcca ctgcgcctgg
    67021 cttgctgagt ttgatacgca cccacggcga gttgaagcgt gctaacagca gctctgccac
    67081 ctcttccgcc acgcgctcca ccagcgcaaa acgtgccccc tcgacgtggc tgaccaccgt
    67141 ttctgcaatg tcagcgtagc tgaggcaatc cgccacatta tcgctttttg ccgctttacg
    67201 gttatcccac gccatttcga tatcgaacac taatttctgt tcgatggtct gttcccagtc
    67261 gtaaacacca atagtggtga ttaccgaaag ttgctctata aatacaatat ccatcacgac
    67321 ctgctcgctt tttggctaac ccggatacca cttccggcga aatgtgcgta ttatccacag
    67381 attcatcgtt taacacgaat tttcaaaacg gaacagctta tgagtgcaat cgcgcctgga
    67441 atgatcctca tcgcgtacct ctgcggctcc atttccagtg ccattctggt ttgccgcctg
    67501 tgtgggctgc ccgatccgcg aaccagcggc tccggtaatc ctggcgcaac taacgtgtta
    67561 cgtataggtg gcaaaggagc agccgtagca gtactgattt tcgacgttct gaaaggaatg
    67621 ttgcccgtct ggggcgcgta tgaattaggt gtcagcccct tctggctagg cttaattgcc
    67681 atcgccgcct gtcttggaca catctggccc gttttcttcg gatttaaagg aggaaaaggc
    67741 gttgctaccg cttttggtgc catcgcaccc attggctggg atctcaccgg agtaatggcg
    67801 ggaacctggt tactgaccgt gctattgagc ggatactcgt cgctgggagc gattgtcagt
    67861 gcactgattg ctccgtttta tgtctggtgg tttaagccac aattcacctt cccggtttcg
    67921 atgctctctt gcctgatcct gctgcgtcat catgacaaca tccagcgtct gtggcgtcgt
    67981 caggagacaa aaatctggac gaaattcaaa agaaagcgcg aaaaggatcc cgagtgattt
    68041 ctggtggatc tacatgacct gatagccttc atcgggtttg cccagccgtt gctggcacca
    68101 tgccgccaga aattccacgc agacacgtaa tttcatgctg cgatagagcg gctcccggta
    68161 aacagcccag atattggcgc tttgtgcata ctccggcaat acttgcacca gtttgccact
    68221 ctccagaaac ggcaacacat cccactcgga acgcagcata atccctttgc cctccagcgc
    68281 ccattgcagc acaatctcgc cgctattgga ggaaagatgc ccgctaacct tcaccgattt
    68341 tttctcctga ccgttcccca actcccatat tccgtgggtc atatcgcgct ctttggtcac
    68401 cagacagtca tgacgactta attcctgtag ggattgtggc tgcggatatt tttgcagata
    68461 ctcaggtgct gcgcataata ttcttttatt tttcgttaac agatgcgcaa tataataatc
    68521 aggaatttcg tcattaatac gaatatccag atcaatatta tcctgcacta aatcaatttg
    68581 ccgatcgaac aattcaaaat acacctgtaa ctcaggataa ttgcgcataa gttcggtaat
    68641 ggctggcgca atatggctgc gcccaaaacc aaaactgcaa ccaatacgaa tcatcccctc
    68701 cgggcgcgtt ttgatttgcg tgacgtcatc gaccagtcgc tgatattggg tgaagatttc
    68761 cagcgcatgt tcgtaacaac gttggccact ttcagtcagc gccacgccgc gagcggagcg
    68821 gttcagcagc gttgttgcca gcgtattttc aaggatttgg atccgcttgg tgacaaatgc
    68881 aggcgtttgc ccaagcgtcg ccgctgcagc actaaaactc ccgctgtgga cgatttctac
    68941 cagtacctgc aagtctttgg ctaaaggcca gctattcagc atcgttaatt atccgcagtt
    69001 gtgataagcg cagtgtattt cgcaaaacat tgccattccc tgctctggcg caatattcat
    69061 tcttattttc gcgttgattc acgaactgtt aattacatat tcacggtggc aaaaaatata
    69121 aaaccacatt tttagtggta gtttgtggcg gtgaattttt ccagacaaat acaaaaactg
    69181 gagttgccat gatgagcgaa agtaataagc aacaggcagt gaataagttg acggagattg
    69241 tcgctaactt taccgccatg atttctaccc gaatgcctga tgacgtggtg gataaactaa
    69301 aacagctaaa ggatgccgaa acgtcgtcga tggggaaaat tatctaccat acgatgttcg
    69361 acaacatgca aaaagcgatt gacctgaatc gtcctgcctg tcaggacacc ggggagatta
    69421 tgttcttcgt taaagtcggt tcccgcttcc cactgcttgg cgagctgcaa agcatactca
    69481 aacaagccgt ggaagaggcg accatcaaag cgccgctgcg tcacaatgcg gtagaaattt
    69541 ttgacgaagt aaacaccggc aaaaataccg gtagcggcgt accgtgggtc acctgggata
    69601 tcgtccccga cggtgacgat gcggaaatcg aagtttacat ggcaggcggc ggctgcacgc
    69661 taccaggccg ctcgaaagtg ttaatgccgt cagaaggcta cgaaggcgtg gtgaaattcg
    69721 tcttcgaaaa tatctccacc ctcgcagtaa acgcctgtcc accggtactg gtgggcgtgg
    69781 gcatcgccac ctcggtggaa accgccgccg tactctcgcg taaagccatt ttgcgcccga
    69841 ttggcagccg ccaccccaat ccaaaagcgg cagagctgga gctacgcctg gaagaaggac
    69901 tcaaccgtct ggggattggt ccacaagggc tgactggcaa cagttcagtg atgggcgtgc
    69961 atatcgaatc tgccgcccgc catccgtcaa ccatcggcgt tgctgtttct accggttgct
    70021 gggcgcatcg tcgcggcaca ctgctggttc atgccgatct cacctttgaa aatctgtctc
    70081 acacccggag cgcgttatga aaaagatcct gacaaccccg atcaaagctg aagatctgca
    70141 agatattcac gtcggcgatg tgatctatct gaccggtacg ctggtgacct gccgtgacgt
    70201 ttgtcaccgc cgattgatcg aactgaaacg tccgctccct tacgatctca acggcaaagc
    70261 gattttccac gctggcccca tcgtgcgcaa aaacggcgac aaatgggaga tggtctccgt
    70321 cggtccgaca accagtatgc gtatggaaag ttttgaacgt gaatttatcg agcagaccgg
    70381 cgtgaaactg gtggtcggca aaggcggaat ggggccgctg accgaagaag gctgtcagaa
    70441 attcaaggcg ctacatgtaa ttttcccggc aggctgcgcg gtgctggcgg caacccaggt
    70501 ggaagagatt gaagaagtgc actggacaga actcgggatg ccggagtcac tgtgggtctg
    70561 ccgggtcaaa gagttcggcc cgctgattgt ctctattgat acccacggca acaacctgat
    70621 agccgaaaac aaaaagctgt tcgccgaacg ccgcgatccc atcgtggaag agatctgcga
    70681 gcacgtccat tacatcaaat aaccctcccg gagaggctcg ccccctctcc tttttcgcag
    70741 gcataacacg atgaaacctt ccactgaatg gtggcgatac cttgcgccgc tggcggtcat
    70801 cgccattatt gctctaattc cggttcccgc agggctggag agtcatacct ggctctactt
    70861 tgccgttttt actggcgtaa tcgttggact gatcctcgaa cccgtgccgg gtgccgtggt
    70921 ggcgatggtg ggtatctcca tcatcgccat actctctccc tggctgctgt tcagcccgga
    70981 gcagctcgct cagccaggct ttaaattcac tgcaaaatcc ctctcgtggg ccgtttccgg
    71041 tttttctaat tcggttatct ggctgatttt cgccgccttt atgtttggca caggctatga
    71101 aaaaaccggg ctggggcgac gtatcgcgct gattctggtg aaaaagatgg ggcatcgcac
    71161 gctgtttctt ggctatgcgg tgatgttctc cgagctgatc ctggcacctg tcacaccgtc
    71221 caactcggcg cgtggtgcgg ggattatcta tcccatcatc cgtaacctgc cgccgctcta
    71281 tcaatcacaa ccaaacgaca gcagttcgcg cagtattggc tcgtacatca tgtggatggg
    71341 gattgttgcc gactgcgtga ccagcgccat tttcctgacg gcgatggctc ctaacttgct
    71401 gttaattgga ctgatgaaaa gcgcatctca cgccacgctg agttggggcg actggttcct
    71461 cgggatgttg ccgctcagta ttttactggt cctgctggtt ccctggctgg cttacgtgct
    71521 gtacccgcct gtactgaagt caggcgatca ggtgccgcgc tgggcagaga cggaactgca
    71581 ggcaatgggc ccgctctgtt cgcgtgaaaa acggatgctg gggctgatgg taggcgcgct
    71641 ggtgctgtgg attttcggcg gtgattatat cgatgccgcg atggtcggtt acagcgtggt
    71701 ggcgctgatg ctgcttctgc gcattatcag ttgggacgat attgtcagta ataaagcggc
    71761 gtggaacgtt ttcttctggc tggcctcgct tatcaccctc gcgaccggac tcaacaacac
    71821 cggttttatt agctggtttg gcaaactgtt agcaggcagc ttaagcggtt attcgccaac
    71881 gatggtgatg gtggcgttga ttgtggtgtt ttatctactg cgctactttt ttgccagcgc
    71941 cacggcgtat acctccgctc tcgcaccgat gatgattgcc gctgcgctgg cgatgccgga
    72001 aatcccgctg ccggtgttct gcctgatggt tggtgcggca attggtctgg ggagcattct
    72061 tacaccatac gccaccggcc ccagtccgat ttactacggc agtggttatc tgccaacggc
    72121 ggattactgg cgactggggg cgatttttgg gctgatattc ctcgtattgc tggtgattac
    72181 cggcttactg tggatgcccg tggtgttgct ttaagtaagg acggtgccgg aagtaactct
    72241 ccggcacccg atagttttac gcagccggta actccgccag cggccagcgc ggacgcacgc
    72301 taacgccgag atccgccgtc gcgcctgctt taaaccgcac cattccggca taggcgatca
    72361 tcgcgccgtt atcagtacaa aattccggac gcgcgtagaa cacttcgccg cggcgttttt
    72421 tcatcatttc agccagcttc gcccgtaacg tgcggttagc actcacgccg cccgccatga
    72481 ccagtcgctt aaagcccgtc tgatccagcg cacgcttaca cttaatcatc aacgtatcga
    72541 ccaccgcatc ttcaaaggcg cgggcgatgt cagcacgcgt ctggtcgtcg gtgccgttgt
    72601 cacgaatggt atttgccgcg aaggttttca ggccggagaa gctgaaatcc agccccggac
    72661 ggtcggtcat cggacgcggg aaaacaaagc gcccggcagt accctgcgcc gccattttcg
    72721 acagtaacgg tccgccagga taatccagcc ccagcagctt cgcggtttta tcaaacgctt
    72781 caccggcggc atcatcgata gactcgccga gcagctcgta ctgaccaatg ccagtcacgc
    72841 tgattaactg cgtatgaccg ccggaaacca gcagcgcaac aaacggaaat tccggcgggt
    72901 tatcttccag catcggcgct aacagatgcc cttccatatg gtgtacaggg atcgccggaa
    72961 cgttccaggc aaacgccaga gaacgcccca cggtcgcgcc aaccagcagt gcgccgacta
    73021 atccagggcc tgcggtatag gccacagcat caatgtcttt tgccgttaaa ccagactcct
    73081 ttagcgccgc ctggatcaac ggtacggttt tacgcacatg atcgcgggag gccagttcag
    73141 gcacaacgcc cccgtagtca gcgtgcaatt tcacctgact atacaattgg ttggctaaca
    73201 aacctttttc atcgtcgtaa atggcgatgc cggtttcatc gcaggaagtt tcaataccca
    73261 gtacacgcat gacttgtttt acctcgcttt attaccgcgc agtgtaggac caatgcgggt
    73321 tgatgtaaaa ctttgttcgc ccctggagaa agcctcgtgt atactcctca cccttataaa
    73381 agtccctttc aaaaaaggcc gcggtgcttt acaaagcagc agcaattgca gtaaaattcc
    73441 gcaccatttt gaaataagct ggcgttgatg ccagcggcaa accgaattaa tcaaaggtga
    73501 gaggcacatg ccggtaatta aagtacgtga aaacgagccg ttcgacgtag ctctgcgtcg
    73561 cttcaagcgt tcctgcgaaa aagcaggtgt tctggcggaa gttcgtcgtc gtgagttcta
    73621 tgaaaaaccg actaccgaac gtaagcgcgc taaagcttct gcagtgaaac gtcacgcgaa
    73681 gaaactggct cgcgaaaacg cacgccgcac tcgtctgtac taattccccg agagcgttgc
    73741 tctccgatca gaccgagttg tagttgtaag gccgtgcttc cgaaaggaat gcgcggctta
    73801 ttttcgttta tgaattgcta aaaatcgggg cctatggctg gacgaatccc acgcgtattc
    73861 attaatgatc tgctggcacg cactgacatc gtcgatctga tcgatgcccg tgtgaagctg
    73921 aaaaagcagg gcaagaattt ccacgcgtgt tgtccattcc acaacgagaa aaccccgtcc
    73981 ttcaccgtta acggtgagaa acagttttac cactgctttg gatgtggcgc gcacggcaac
    74041 gcgatcgact tcctgatgaa ctacgacaag cttgagttcg tcgaaacggt cgaagagctg
    74101 gcggcaatgc acaatcttga agtgccattt gaagcaggta gcggccccag ccagatcgag
    74161 cgccatcagc ggcaaacgct ttatcagttg atggacggtc tgaatacgtt ttaccaacaa
    74221 tctttacaac aacctgttgc cacgtctgcg cgccagtatc tggaaaaacg cggattaagc
    74281 cacgaggtta tcgctcgctt tgcgattggt tttgcgcctc ccggctggga caacgtcctg
    74341 aagcggtttg gcggcaatcc agaaaatcgc cagtcattga ttgatgcggg gatgttggtc
    74401 actaacgatc agggacgcag ttacgatcgt ttccgcgagc gggtgatgtt ccccattcgc
    74461 gataaacgcg gtcgggtgat tggttttggc gggcgtgtgc tgggcaacga tacccccaaa
    74521 tacctgaact cgccggaaac agacattttc cataaaggcc gccagcttta cggtctttat
    74581 gaagcgcagc aggataacgc tgaacccaat cgtctgcttg tggtcgaagg ctatatggac
    74641 gtggtggcgc tggcgcaata cggcattaat tacgccgttg cgtcgttagg tacgtcaacc
    74701 accgccgatc acatacaact gttgttccgc gcgaccaaca atgtcatttg ctgttatgac
    74761 ggcgaccgtg caggccgtga tgccgcatgg cgagcgctgg aaacggcgct gccttacatg
    74821 acagacggcc gtcagctacg ctttatgttt ttgcctgatg gcgaagaccc tgacacgctg
    74881 gtacgaaaag aaggtaaaga agcgtttgaa gcgcggatgg agcaggcgat gccgctttcc
    74941 gcatttctgt ttaacagcct gatgccgcaa gttgatctga gtacccctga cgggcgcgca
    75001 cgtttgagta cgctggcact gccattgata tcgcaagtgc cgggcgaaac gctgcgaata
    75061 tatcttcgtc aggaattagg caacaaatta ggcatacttg atgacagcca gcttgaacga
    75121 ttaatgccaa aagcggcaga gagcggcgtt tctcgccctg ttccgcagct aaaacgcacg
    75181 accatgcgta tacttatagg gttgctggtg caaaatccag aattagcgac gttggtcccg
    75241 ccgcttgaga atctggatga aaataagctc cctggacttg gcttattcag agaactggtc
    75301 aacacttgtc tctcccagcc aggtctgacc accgggcaac ttttagagca ctatcgtggt
    75361 acaaataatg ctgccaccct tgaaaaactg tcgatgtggg acgatatagc agataagaat
    75421 attgctgagc aaaccttcac cgactcactc aaccatatgt ttgattcgct gcttgaactg
    75481 cgccaggaag agttaatcgc tcgtgagcgc acgcatggtt taagcaacga agaacgcctg
    75541 gagctctgga cattaaacca ggagctggcg aaaaagtgat ttaacggctt aagtgccgaa
    75601 gagcgatcgg gaagcccccg acagccgcac tgagaggcag cggcaaatat ataagtacgc
    75661 cctcgtaatt atcgttggcg gtaaacaacc gttggatttc agcgttaacg gctgaaggac
    75721 atcgggtcaa tcgcccaaca ccaacctcat gaaataagtg tggataccgt cttatggagc
    75781 aaaacccgca gtcacagctg aaacttcttg tcacccgtgg taaggagcaa ggctatctga
    75841 cctatgccga ggtcaatgac catctgccgg aagatatcgt cgattcagat cagatcgaag
    75901 acatcatcca aatgatcaac gacatgggca ttcaggtgat ggaagaagca ccggatgccg
    75961 atgatctgat gctggctgaa aacaccgcgg acgaagatgc tgccgaagcc gccgcgcagg
    76021 tgctttccag cgtggaatct gaaatcgggc gcacgactga cccggtacgc atgtacatgc
    76081 gtgaaatggg caccgttgaa ctgttgaccc gcgaaggcga aattgacatc gctaagcgta
    76141 ttgaagacgg gatcaaccag gttcaatgct ccgttgctga atatccggaa gcgatcacct
    76201 atctgctgga acagtacgat cgtgttgaag cagaagaagc gcgtctgtcc gatctgatca
    76261 ccggctttgt tgacccgaac gcagaagaag atctggcacc taccgccact cacgtcggtt
    76321 ctgagctttc ccaggaagat ctggacgatg acgaagatga agacgaagaa gatggcgatg
    76381 acgacagcgc cgatgatgac aacagcatcg acccggaact ggctcgcgaa aaatttgcgg
    76441 aactgcgcgc tcagtacgtt gtaacgcgtg acaccatcaa agcgaaaagt cgcagtcacg
    76501 ctgccgctca ggaagagatc ctgaaactgt ctgaagtatt caaacagttc cgcctggtgc
    76561 cgaagcagtt tgactacctg gtcaacagta tgcgcgtcat gatggaccgc gttcgtacgc
    76621 aagaacgtct gatcatgaag ctctgcgttg agcagtgcaa aatgccgaag aaaaacttca
    76681 ttactctgtt taccggcaac gaaaccagcg atacctggtt caacgcggca attgcgatga
    76741 acaagccgtg gtcggaaaaa ctgcacgatg tctctgaaga agtgcatcgc gccctgcaga
    76801 aactgcagca gattgaagaa gaaaccggcc tgaccatcga gcaggttaaa gatatcaacc
    76861 gtcgtatgtc catcggtgaa gcgaaagccc gccgtgcgaa gaaagagatg gttgaagcga
    76921 acttacgtct ggttatttct atcgccaaga aatacaccaa ccgtggcttg cagttccttg
    76981 acctgattca ggaaggcaac atcggtctga tgaaagcggt tgataaattc gaataccgcc
    77041 gtggttacaa gttctccacc tacgcaacct ggtggatccg tcaggcgatc acccgctcta
    77101 tcgcggatca ggcgcgcacc atccgtattc cggtgcatat gattgagact atcaacaaac
    77161 tcaaccgtat ttctcgccag atgctgcaag agatgggccg tgaaccgacg ccggaagaac
    77221 tggctgaacg tatgctgatg ccggaagaca agatccgcaa agtgctgaag atcgccaaag
    77281 agccaatctc catggaaacg ccgatcggtg atgatgaaga ttcgcatctg ggggatttca
    77341 tcgaggatac caccctcgag ctgccgctgg attctgcgac caccgaaagc ctgcgtgcgg
    77401 caacacacga cgtgctggct ggcctgaccg cgcgtgaagc gaaagttctg cgtatgcgtt
    77461 tcggtatcga tatgaacacc gatcacacgc tggaagaagt gggtaaacag ttcgacgtta
    77521 cccgtgaacg tatccgtcag atcgaagcga aggcgctgcg caaactgcgt cacccgagcc
    77581 gttctgaagt gctgcgtagc ttcctggacg attaatcggt aggccggatc aggcgttacg
    77641 ccgcacccgg cacctgtccc tttgcacaaa cgccaccttt tcggtggcgt tttttatcgc
    77701 ccacgcacca ccagcgcctg gtccagctcg cgatacgctt caaccagttt ctccagtgaa
    77761 acgcgactta aaccgctggg atttggcagc acccaaatct gcgtcgaacc aatgctgagc
    77821 gtttgtttcc cccactgtgc accacgctgg ctgaatccct gttcatatgc ttgtttgccc
    77881 agaatcgcca acgcttgcgg ctgataatct tcaatttttt caatcagctt acgcccacct
    77941 gcgtgtagct cttgctttga aatttcattg gcttgcaccg ttggcctgtc taccagtttg
    78001 gtgacgccac aacgataatc cagcagatgc tgtgcctcct gcggcttcaa ctgatggtcg
    78061 gtaaacccgg cctgatatat caccttccag aagcgatttg ccggatgagc aaagggaaaa
    78121 ccagtcccgg cggatgaaag cccagggttg ataccgcaaa acacgacccg taaccctgga
    78181 gccaaaatat cctcaaccat atttactcct ttctaacatc accaggaaaa gtataacgga
    78241 ttgaaaatac attgtttata aaaacagcag gcgcgcggta atggctggat tgcgacacgg
    78301 agttacttta taatccgcta ccatggcccc ttagctcagt ggttagagca ggcgactcat
    78361 aatcgcttgg tcgctggttc aagtccagca ggggccacca gatatagcaa aggctgacga
    78421 gaaatcgtca gcctttttct ttttatatat cagttacttt gcgtgccagt aggccgctgc
    78481 acgtacccgc tgtgggtcat actgttccgc ttcaaagcgg cggcttaaat tcttaacgac
    78541 tttaccttcg ccggttatcc agatgaagta atcatcggca gggatttgca tctgcgccag
    78601 acgcgcatct accgcctgct catcatgtgc cagccattcg atattaaaac catcaaggtg
    78661 cgcgagataa tcctgacagg cgttatcccg cacgctaacc agcgcactaa cttgcggttt
    78721 aacggcaagt ttgctcaacg tttccaggcg gcggcgcaat gcgggcattc cggattcatc
    78781 gcagacatac agctgatacg cgtaatcttc cggcaccacc agcgaaccgc gcggacctgc
    78841 caccgtaagt ttatcgcccg gttgcgcctg catcgcccag ccgctggcga ccccaccgtc
    78901 gtgaataaag aaatcgatcg ccagttcatg gcgtagttcg tcatacagcg gcgtatagtc
    78961 acgcgacggt gggcgtggtc cttccggcca gacgatgccc tcttccgtta ccgttggcgg
    79021 cacaaagtga gcgtcaggtt gaggaaagaa gagtttgctg tgatcgtcaa agccgcgcga
    79081 tgtaaaaccg tccagcgcct cgccgccaag gacaatgcgc tgaaaaccgg cgctgatacg
    79141 ctcaacgcgt agcacagtca attcacggaa gcgcagatca ttgcgaacgc gctgcgggta
    79201 gcggggggag ttattcattg ttatcgcctt cgtgatggta atcagatata tctaaataat
    79261 tttcgcaaat gataatgatt gttaatcatg ataaatgcaa gcgatttgta gaactgatat
    79321 gtctatagtc tgacaagacg aaccgcctct tctcaggcat cattactcaa cgccggatgc
    79381 ggcgtgaacg ccttatccgg cctacgtgtg agatgagtcc acatttaaaa atcattttta
    79441 ttcttgcaag agtgctcaca tcagatataa attagatata tctaattaag caaaaggagg
    79501 ctgatatgag ccatcatcac gaagggtgtt gtaaacatga aggccagcca cgccatgagg
    79561 gctgctgcaa aggtgagaag tcagaacacg agcactgcgg acacggtcac cagcatgaac
    79621 acggtcaatg ttgcggtggt cgccacggtc gcggcggcgg tcgtcggcaa cgtttctttg
    79681 gtcacggtga attgcgtctg gtgattctgg atattctctc gcgcgatgac agccacggtt
    79741 acgaattgat taaagcgatt gagaatctaa cccaagggaa ttacacccca agcccgggcg
    79801 tcatctaccc gacgctggat tttctgcagg agcagtcgct gattaccatc cgcgaagagg
    79861 aaggtggtaa gaagcagatt gcgctgaccg aacaaggcgc gcagtggctg gaagaaaacc
    79921 gcgaacaggt ggagatgatt gaagaacgca tcaaagcgcg ttgcgttggc gctgcgctgc
    79981 gccagaaccc gcaaatgaag cgggcgctag ataattttaa agcggtgctg aatttacgcg
    80041 tcaaccagag tgatatcact gatgcacaaa taaaaaagat cattgcggtg atcgaccgcg
    80101 ccgcttttga tattacgcaa ctggattaat cgccgcatcc gccagtggcg cggtgcaatt
    80161 gccggatgcg acgcttgacg caccttatcc ggcctacacc cgctacacac cccgtaggcc
    80221 tgataagatg cgccagcatc gcatcaggca tcgtgtatca aataccggat ccggcatacc
    80281 gattaatgca gcaccgtcac cgcgtcttcc agtcggctgg cgcggtgttt caccatcgcc
    80341 gacacctgcg cactctcttc caccagctcg gcatttttct gggtgatgag gttaagctca
    80401 tccactgcac gggtcaggct ggaaagacca tcggcctgtt ccagcgttga atggctaatc
    80461 tgggcaatca actgggtgac gtttttcacc tgtgccacaa tatcttccat cgtacgtccg
    80521 gcggcgtgta cctgctgcga accggattgc accttatcag cactggcatc aatcagcttg
    80581 cgaatatcgt tggcagcatt ggcgctgcgg ctggctaaat gacgcacttc ccccgccacc
    80641 accgcaaaac ctttgccctg ttcgccggca cgcgccgctt ccaccgccgc attcagggcc
    80701 agaatattgg tctgaaacgc aatatcgtta atcagcgaag taatggtgcc aatgcgctgg
    80761 gtactgtcgg cgatatcgtc cattgtcttg atcaccgtgg tcatcgcctc cccgccctgc
    80821 actgccgcat tactggcggt gattgacagt ttatcggcag ccgacgccgt ggcagagttc
    80881 tgtttcaccg acgccgccat ttggttcatg gtcgccaccg tttgctgaac gttatcaact
    80941 gtctgctggg tatgttcgtt cagttcatcg gtgcctttcg ccagcgtctc actgccgttt
    81001 ctgacactgg acacctggct tgagacatcg ttaattaacc aacggcacat caggccaagc
    81061 tgccctaccg cgcgtaatgt cagccccagc tcatcgctgc gattcagatg ctcaacacta
    81121 ttacgctctc cggtcgccac cttcagtgcc tgacgggcga cattttctat cgggcggaca
    81181 atctgccatt caaaacaggc gcttgccaac aataccacta acgcacagag gatatacgtc
    81241 accaccgggg cagcaacaaa ccaaagcatg gccgccagca agataaacat cagggtcatc
    81301 actccacgcg cccgccagcg aagcggtaat gaaggcagtt tacccagcca gcctttacgc
    81361 accaccaggc ctttatgaat acgcttaccg gtacgtccgg cgttcagcgc tttgtacagc
    81421 ggctccaccg ccgcgatctc ttcatccgtc gcccgggtac gaatcgacat atagccactg
    81481 atttttccct cgcgcaccat cggtaccgca ttggcccgca cccaataatg gtcaccattt
    81541 ttgcggcgat ttttcacgat gccgctccag ggctcccctt ttttcagggt gaaccacata
    81601 tccgcaaacg ccgcttttgg catatccggg tgacgcacca tgttgtgcgg ctgcccttgt
    81661 aactcttgca aggtaaagcc gctcacctgc acaaaagtgt cattagcatg agtgatatag
    81721 ctttgcagat cggtagtgga catcagagtg gtatcgtccg ccagcggggt attttgctgg
    81781 gtgacatacg gatgagaaga catgttcgcg tcctgtgcag gttatatggt tgttaacttc
    81841 ttgtcagagt ttatgtcggc cccgctgcgg ttatctttaa ccgattaatt tgatttagat
    81901 cgcaatttgc gattaaagca caaatctaat tcgttgattt aaaatacttt cactctgtta
    81961 ctatacgaaa acgttaatta tcttgcccaa aaatcaggca gttaatgccc tgaaagcgtg
    82021 catttgcgca gcaattatca aatccatacc cgacaaaaac cgtgcaaaat aacaacagat
    82081 gttaacaaat agcattaaat attgcgcaaa tgataaccac atttgtgttt atcccgattt
    82141 tcgcgatcgc agccggagtg gcgcaatccc tgcaatactt aaatcggtat catgtgatac
    82201 gcgagcctcc ggagcatatt ttgaacaggt taccttcgag cgcatcggct ttagcgtgca
    82261 gcgcccacgc cctgaatctc attgaaaagc gaacgctgga tcatgaggag atgaaagcac
    82321 ttaaccgaga ggtgattgaa tacttcaaag agcatgtcaa tccggggttt ttagagtatc
    82381 gcaaatctgt taccgccggc ggggattacg gagccgtaga gtggcaagcg ggaggtttaa
    82441 atacgcttgt cgacacccag ggacaggagt ttatcgactg cctgggaggt tttggaattt
    82501 tcaacgtggg gcaccgtaat ccagttgtgg tttccgccgt acagaatcaa cttgcgaaac
    82561 aaccactgca cagccaggag ctactcgatc cgttacgggc tatgctggcg aaaacccttg
    82621 ctgcgctaac gccaggtaaa ctgaaataca gcttcttctg taatagcggc accgagtccg
    82681 ttgaagcggc gctgaagctg gcgaaggctt atcagtcgcc gcgcggcaaa tttactttta
    82741 ttgccaccag cggcgcgttc cacggtaaat cacttggcgc gctgtcggcc acggcgaaat
    82801 cgaccttccg caaaccgttt atgccactgc tgccagggtt ccgtcatgtg ccgtttggca
    82861 atatcgaagc catgcgcacg gctcttaatg agtgcaaaaa aaccggtgat gatgtggccg
    82921 cggtgatcct cgaaccgatt cagggcgaag gtggcgtaat tctgccgccg ccgggctatc
    82981 tcaccgccgt gcgtaagctg tgcgatgagt tcggcgcact gatgatcctc gatgaagtac
    83041 aaacgggcat ggggcgcacg ggcaagatgt tcgcctgcga acatgagaac gtacagccgg
    83101 acatcctgtg cctggcaaaa gccctcggcg gcggtgtgat gccgattggc gcaaccatcg
    83161 ccactgaaga ggtgttctca gttctgttcg acaacccgtt cctgcatacc accacctttg
    83221 gcggcaaccc gctggcctgt gcggcggcgc tggcgaccat caatgtgttg ctggagcaga
    83281 acttaccggc tcaggctgag caaaaaggcg atatgttgct ggacggtttc cgtcaactgg
    83341 cgcgggaata tcccgatctg gtacaggaag cgcgtggtaa agggatgtta atggcgattg
    83401 agtttgttga taacgaaatc ggctataact ttgccagcga gatgttccgc cagcgcgtac
    83461 tggtggccgg aacgctcaat aacgccaaaa cgatccgcat tgaaccgcca ctgacactga
    83521 ccattgaaca gtgtgaactc gtgatcaaag cggcgcgtaa ggcgctggcg gccatgcgag
    83581 taagtgtcga agaagcgtag tatcaaatcg gatggcaata cgacgtcgcc atccgatctt
    83641 tttatacgat acgtacgccc gcaggcatca tccgctccgg cgttaacagc acgctttcac
    83701 tgccgtcatc ggtttccgca cacaacagca tgcattccga cgtttcacca cgcattttcg
    83761 ctttttgcag attgcataag accaccaccg tttttcccat caactcttct tcgctgtagt
    83821 acggcaccag gctggtcacg gtttgcagtg ttttttcccc cacatcaacc tgtacgatgt
    83881 acagcttgtc ggcgttttca tggcgtttca cttccacaat ctttccgacg cgcatttcca
    83941 gacgtgcaaa atcagcgtaa gccacggttt ccattgcttc ctcccttcga gtaaaatttt
    84001 actaaactat agcaaaagtt tttctcaatc ctgtaggcta aaaatggaga atgcaggcgt
    84061 gatcacattc gtgagccgct gtgttaccgt tacagcgtca aagaaacgcg ctttatttac
    84121 tgaaaacagg tgacccgata agcacttcct ctacaatggg ggcgcacatc agggaaagta
    84181 aaaaaggtaa acatggcaac actaaaagac atcgcaatcg aagctggcgt atccctggcg
    84241 acagtatcca gggtcttaaa tgacgatccg acattgaatg tgaaagaaga gacgaaacat
    84301 cgcattctcg agatcgccga aaagctggag tacaagacca gtagcgcccg taaactacag
    84361 acaggcgcag tcaaccaaca ccatattctg gctatctaca gctaccagca ggagctggag
    84421 atcaacgatc cttactatct ggcgatccgc cacggcattg aaactcagtg cgaaaagctg
    84481 gcgatcgaac tgaccaactg ttatgaacac agcggattac cagacattaa aaacgtcacc
    84541 ggtattttaa ttgtcggcaa acccacgccc gccctgcgcg ccgctgccag cgcgttgacc
    84601 gacaatatct gttttatcga ctttcacgaa cccggcagcg gttacgatgc ggtggatatc
    84661 gatctggcac gcatcagtaa agaaatcatc gacttctata tcaaccaggg cgttaatcgt
    84721 attggtttta ttggcggtga agatgagcct ggcaaggcgg atattcgtga ggtcgccttt
    84781 gcggaatatg gccgactgaa acaagtggta cgcgaagagg atatctggcg cggcggtttt
    84841 tccagttcgt cgggttatga actggcaaaa caaatgctgg cgcgggaaga ctatccgaag
    84901 gcactgtttg ttgcttccga ttccattgct atcggcgtac tgcgggcgat tcatgaacgt
    84961 ggtctgaaca tcccacagga tatttcgctt atcagcgtta acgatatccc caccgcgcga
    85021 tttacctttc cgccgctctc caccgtgcgc atccattccg aaatgatggg aagtcagggc
    85081 gtaaacctgg tgtatgaaaa agcccgcgat ggtcgcgcgc tgccgctgtt agtcttcgtt
    85141 cccagcaaat taaaactgcg cggcacgacc cgttaaatcc ccttacacac tgtccggcaa
    85201 tcgtttttgc cgggcagtgc tgccgtttat tttcgtgatc cagttaaagt aaatgcattt
    85261 acccgctact ttttagtaaa aattttacta aactccccag caattacaca aactaccatc
    85321 accatgaatg gttccgattt ctctctaccg ggaggcccta tgaatcgctg ggaaaacatt
    85381 cagctcaccc acgaaaaccg acttgcgccg cgtgcgtact ttttttcata tgattctgtt
    85441 gcgcaagcgc gtacctttgc ccgcgaaacg agcagcctgt ttctgccctt aagcggtcag
    85501 tggaatttcc acttttttga ccatccgctg caagtgccag aagccttcac ctctgagtta
    85561 atggctgact gggggcatat taccgtcccc gccatgtggc aaatggaagg tcacggcaaa
    85621 ctgcaatata ccgacgaagg ttttccgttc cccatcgatg tgccgtttgt tcccagcgat
    85681 aacccaaccg gtgcctatca acgtattttc accctcagtg acggctggca gggtaaacag
    85741 acgctgatta aatttgacgg cgtcgaaacc tattttgaag tctacgttaa cggtcagtat
    85801 gtgggtttca gcaagggcag tcgcctgacc gcagagtttg acatcagcgc gatggttaaa
    85861 accggcgaca acctgttgtg tgtgcgcgtg atgcagtggg cggactctac ctacgtggaa
    85921 gaccaggata tgtggtggtc agcggggatc ttccgcgatg tttatctggt cggaaaacac
    85981 ctaacgcata ttaacgattt cactgtgcgt accgactttg acgaagccta ttgcgatgcc
    86041 acgctttcct gcgaagtggt gctggaaaat ctcgccgcct cccctgtcgt cacgacgctg
    86101 gaatataccc tgtttgatgg cgaacgcgtg gtgcacagca gcgccattga tcatttggca
    86161 attgaaaaac tgaccagcgc cagctttgct tttactgtcg aacagccgca gcaatggtca
    86221 gcagaatccc cttatcttta ccatctggtc atgacgctga aagacgccaa cggcaacgtt
    86281 ctggaagtgg tgccacaacg cgttggcttc cgtgatatca aagtgcgcga cggtctgttc
    86341 tggatcaata accgttatgt gatgctgcac ggcgtcaacc gtcacgacaa cgatcatcgc
    86401 aaaggccgcg ccgttggaat ggatcgcgtc gagaaagatc tccagttgat gaagcagcac
    86461 aacatcaact ccgtgcgtac cgctcactac ccgaacgatc cgcgttttta cgaactgtgt
    86521 gatatctacg gcctgtttgt gatggcggaa accgacgtcg aatcgcacgg ctttgctaat
    86581 gtcggcgata tcagccgtat taccgacgat ccgcagtggg aaaaagtcta cgtcgagcgc
    86641 attgttcgcc atattcacgc gcagaaaaac catccgtcga tcatcatctg gtcgctgggc
    86701 aatgaatccg gctatggctg taacatccgc gcgatgtacc acgcagcgaa ggcgctggat
    86761 gacacgcgac tggtgcatta cgaagaagat cgcgatgctg aagtggtcga tattatttcc
    86821 accatgtaca cccgcgtgcc gctgatgaat gagtttggtg aatacccgca tccgaagccg
    86881 cgcatcatct gtgaatatgc tcatgcgatg gggaacggac cgggcgggct gacggagtac
    86941 cagaacgtct tctataagca cgattgtatt cagggacatt atgtttggga gtggtgcgac
    87001 cacgggatcc aggcgcagga tgacaacggc aatgtctggt ataaattcgg cggcgactac
    87061 ggcgactatc ccaacaacta taacttctgt cttgatggtt tgatctattc cgatcagacg
    87121 ccgggaccag gcctgaaaga gtacaaacag gttatcgcgc cggtaaaaat ccacgcgctg
    87181 gatctgactc gcggcgagct gaaagtcgaa aataaactgt ggtttaccac gctggatgac
    87241 tacaccctgc acgcagaggt gcgcgccgaa ggtgaaacgc tcgcgacgca acagattaaa
    87301 ctgcgcgacg ttgcgccgaa cagcgaagcc cccttgcaga tcacgctgcc gcagctggac
    87361 gcccgcgaag cgttcctcaa cattacggtg accaaagatt cccgcacccg ctacagcgaa
    87421 gccgggcatt ctatcgccac ttatcagttc ccgctgaagg aaaacaccgc gcagcctgta
    87481 cctttcgcac cgaataacgc ccgtccgctg acgctggaag acgatcgttt gagctgtact
    87541 gttcgcggct acaactttgc gatcaccttc tcaaaaacga gtggtaaacc gacatcctgg
    87601 caggtaaatg gcgaatcgct gctgacccgc gagccaaaga tcaacttctt caagccaatg
    87661 atcgacaacc acaagcagga gtacgaaggg ttgtggcagc cgaatcattt gcagatcatg
    87721 caggaacatc tgcgcgactt tgccgtagaa cagagcgatg gtgaagtgtt gatcatcagc
    87781 cgcacggtta tagcaccgcc ggtgtttgac ttcgggatgc gctgtaccta catctggcgc
    87841 atcgctgcag atggccaggt taacgtggcg ctttccggcg agcgttacgg cgactatccg
    87901 cacatcattc cgtgcatcgg tttcaccatg gggattaacg gcgaatacga tcaggtggcg
    87961 tattacggtc gtggaccggg cgaaaactac gccgacagcc agcaggctaa catcatcgat
    88021 atctggcgca gcaccgtcga tgccatattc gagaactatc ccttcccgca gaacaacggc
    88081 aaccgtcagc atgtccgctg gacggcactg actaaccgcc acggcaacgg tctgctggtg
    88141 gttccgcagc gcccaattaa cttcagcgcc tggcactata cccaggaaaa catccacgct
    88201 gcccagcact gtaacgagct gcagcgcagt gatgacatca ccctgaatct cgaccaccag
    88261 ctgcttggcc tcggctccaa ttcctggggc agcgaggtgc tggactcctg gcgcgtctgg
    88321 ttccgtgact tcagctacgg ctttacgttg ctgccggttt ctggcggaga agctaccgcg
    88381 caaagcctgg cgtcgtatga gttcggcgca gggttctttt ccacaaattt gcacagcgag
    88441 aataagcaat gaggatcatc gataacttag aacagttccg ccagatttac gcctctggta
    88501 agaagtggca acgctgcgtt gaagcgattg aaaatatcga caacattcag cctggcgtcg
    88561 cccactccat cggtgactca ttgacttacc gcgtggagac agactccgcg accgatgcgc
    88621 tatttaccgg gcatcgacgc tattttgaag tgcattacta cctgcaaggg cagcaaaaaa
    88681 ttgaatatgc gccgaaagag acattacagg tcgtggaata ttatcgtgat gaaactgacc
    88741 gtgaatattt aaaaggctgc ggagaaaccg ttgaggtcga cgaagggcaa atcgttattt
    88801 gcgatatcca tgaagcatat cggtttatct gcaataacgc ggtcaaaaaa gtagttctca
    88861 aagtcaccat cgaagatggt tatttccata acaaataaca actacggcgg caaaaggagt
    88921 ttgccgccac cgctacccta ctcattttcg gagatgtgtt atgtctgata ccaaacgtaa
    88981 tacaatcggc aaattcggct tgctctcgct gacttttgcc gccgtttaca gctttaacaa
    89041 cgttatcaat aataatattg agcttggact ggcctcggca ccgatgtttt tcctcgcgac
    89101 gattttttat tttattccct tctgtctgat catcgcagaa tttgtttcgt taaataaaaa
    89161 ctcagaagcc ggtgtctacg cgtgggtaaa aagttcgctg ggcggacgtt gggcatttat
    89221 tactgcctat acctactggt tcgtaaacct gttctttttc acctcgctgt tgccgcgcgt
    89281 tattgcttat gcttcgtatg ccttcctcgg ctatgaatat attatgacgc cggttgccac
    89341 caccattatc agtatggtgc tgttcgcctt ctccacctgg gtttccacca acggggcgaa
    89401 aatgttgggg ccaattacct ccgtcacttc aacgctgatg ctgctgttaa cgctctccta
    89461 cattttactg gcaggtacgg cgctggttgg cggcgtacag cctgctgacc ccatcaccgt
    89521 tgacgcgatg atcccgaact tcaactgggc gttcctcggc gttaccacct ggatctttat
    89581 ggccgcaggt ggcgcggagt ccgtcgctgt gtacgttaac gacgtcaaag gcggttcgaa
    89641 atcgttcgtt aaagtgatca tcctcgccgg gatttttatc ggtgtactat attccgtctc
    89701 ctcggtgctg attaacgtct tcgtcagcag caaagagttg aaatttactg gcggatcggt
    89761 gcaggtattc cacggcatgg cggcgtattt tggtctgccg gaagcgttga tgaatcgctt
    89821 tgtcggtctg gtgtccttta ccgcaatgtt cggttccctg ctgatgtgga ccgcaacgcc
    89881 ggtgaaaatt ttcttctccg aaatcccgga aggcatcttt ggtaagaaaa ccgtcgaact
    89941 taacgaaaac ggcgttccgg cgcgcgcagc gtggatccag ttcctgatcg tcatcccgct
    90001 gatgattatc ccgatgctcg gttccaatac tgtgcaggat ctgatgaata ctattattaa
    90061 tatgaccgcc gcagcgtcca tgcttccgcc gttattcatc atgctggctt acctgaattt
    90121 acgcgccaaa ttagatcacc tgccacgcga tttccgtatg ggctcccgcc gcaccggtat
    90181 tatcgttgtt tcaatgctga ttgcgatatt tgccgtaggg tttgtcgctt cgacattccc
    90241 gactggcgcg aatattctga ccatcatttt ttataacgtc ggcggtattg ttatattcct
    90301 tggctttgcg tggtggaaat acagtaaata tataaaggga ttaacggctg aagagcgcca
    90361 tattgaagcg acgccagcca gcaatgttga ttaaacagac taataaaaaa tgagcaggca
    90421 ttttccctct tcctggatga ggagagggaa aatagtttct gcctttatat ttttataatt
    90481 acaacgataa aaggctgtac tttttcttta gctcatggat taacacaatg aaattaatca
    90541 ctgcaccatg cagagcatta cttgctctgc cgttttgcta cgccttttct gcggcaggag
    90601 aagaagcacg tccggcagaa catgacgaca caaaaacccc cgcaattacc tcgacatctt
    90661 ctccttcatt tcgtttttac ggcgaattag gggttggtgg atatatggat ttagagggtg
    90721 agaataaaca taaatacagc gacggcacct atattgaagg tggcctggag atgaagtacg
    90781 gctcctggtt cggcctgatt tacggcgaag gctggaccgt gcaggccgac cacgacggca
    90841 atgcctgggt gcctgaccat agctggggcg gtttcgaagg tgggattaac cgtttctatg
    90901 gcggttatcg aaccaatgat ggcaccgaaa tcatgctcag tctgcgtcag gattcctcgc
    90961 tggatgacct gcaatggtgg ggcgacttca cacccgatct gggctacgtc attcccaata
    91021 cccgcgacat tatgaccgcg ctgaaggtgc agaacttaag cggcaacttt cgttatagcg
    91081 tcaccgccac acctgccgga catcatgacg aaagcaaagc atggctacat tttggcaagt
    91141 acgatcgcta cgacgacaaa tacacctatc cggcaatgat gaacggttac atccagtatg
    91201 accttgccga aggcatcacc tggatgaatg gtctggaaat taccgacggc acaggacaac
    91261 tctatctcac gggactgctt acccctaact ttgccgctcg cgcctggcac cataccggac
    91321 gtgccgacgg gctggacgta ccgggaagtg aaagcgggat gatggtgagc gccatgtatg
    91381 aagcgttaaa gggcgtttat ctctccaccg cttacaccta cgccaaacat cgccctgacc
    91441 acgctgacga tgaaaccacc tctttcatgc agtttggtat ctggtacgaa tatggcggcg
    91501 gacgtttcgc aacggctttt gatagccgct tctacatgaa aaatgcctct aacgatccca
    91561 gcgaccaaat cttcctgatg caatatttct actggtaata aggactgtaa tttcatgaaa
    91621 atcaaaacta ttttaacgcc agtaacctgc gctctgctga taagtttttc cgcccatgcc
    91681 actaacgccg acaattataa aaacgtgatt aaccgtactg gtgcgccgca gtacatgaag
    91741 gattacgatt acgacgatca ccagcgtttt aatccgtttt tcgatctcgg agcctggcat
    91801 ggtcatctgt tgccagacgg cccgaacacc atgggcggct ttccgggcgt tgcgctgctg
    91861 acggaagaat acatcaactt tatggccagc aatttcgacc gcctgaccgt ctggcaggac
    91921 ggcaagaaag tcaacttcac gctggaggca tacaatattc ccggcgcgct ggtgcaaaaa
    91981 ctgacagcaa aagatgtgca ggtcgaaatg actctgcgct tcgccacgcc gcgcacgtca
    92041 ctactggaaa ctaaaatcat cagcaataaa ccgctggatc tggtgtggga tggcgaactg
    92101 ctggaaaaac tggaagcgaa agaagggaaa ccgctttccg ataaaaccat tgctggcgaa
    92161 taccctgact accagcgcaa aatcagcgcc acccgtgatg gcctgaaagt cacctttggc
    92221 aaagtgcgcg ccacctggga tctgctgacc tccggtgaat cagaatatca ggtgcataaa
    92281 tccctgccgg tgcagactga aatcaacggc aatcgcttta ccagtaaggc gcatatcaac
    92341 ggttcgacga cgctctatac cacctattcc catctgctga cctctcagga agttagcaaa
    92401 gagcaaatgc agatccgcga tattctggcg cgtccggcgt tttatctcac cgcctcgcag
    92461 caacgctggg aagagtatct gaagaaaggg ttaaccaatc cggatgcgac gccggaacag
    92521 acacgcgtcg cggtgaaagc gatcgaaacg ctcaacggta actggcgctc gcctgggggt
    92581 gcggtgaaat ataacaccgt cacgccgtcg gtgaccgggc gctggttctc cggcaatcag
    92641 acctggccgt gggatacctg gaagcaggcg tttgcgatgg cgcatttcaa tccggacatc
    92701 gccaaagaga atatccgcgc ggtcttctcc tggcagatcc agcctggcga cagcgtgcgt
    92761 ccgcaggatg tgggctttgt ccccgacctg atagcgtgga atcttagccc cgagcgtggt
    92821 ggcgatggtg gcaactggaa cgaacgaaat accaaaccca gccttgccgc ctggtcggtg
    92881 atggaagtgt acaacgtcac ccaggataaa acctgggtgg cagagatgta cccgaaactg
    92941 gtggcctatc acgactggtg gttacgtaac cgcgatcaca acggcaacgg cgtgccggaa
    93001 tatggcgcga cccgcgacaa agcccacaac actgagagcg gcgagatgct gtttacggtg
    93061 aagaaaggca acaaagaaga gacgcagtct gggctgaaca actacgcccg cgtggtggag
    93121 aaaggccagt acgacagtct ggaaattccg gcacaggttg ctgcatcgtg ggaatcgggg
    93181 cgtgatgatg ccgccgtctt tgggtttatc gacaaagaac agctggataa atatgtcgca
    93241 agcggcggca aacgtagcga ctggacagtg aaattcgccg aaaaccgcag tcaggacgga
    93301 acgttgctgg ggtactcgct attgcaggag tcggtggatc aggccagcta tatgtacagc
    93361 gataaccatt atctggcgga gatggcgacg attctcggta aaccggaaga ggctaagcgc
    93421 tatcgccagt tggcacagca gctcgcggac tacatcaaca cctgtatgtt cgacccggct
    93481 acgcagtact tctatgacgt gcgtattgaa gataaaccgc tggcgaacgg ctgcgcgggc
    93541 aaaccgattg ttgagcgcgg taaagggccg gaaggctggt cgccgctgtt taacggtgcg
    93601 gcaacgcagg ccaatgccga cgcggtggtg aaggtgatgc tcgatcctaa agagttcaat
    93661 acctttgttc cgctgggaac ggcggcgtta accaatccgg cctttggcgc tgatatctac
    93721 tggcgcgggc gcgtatgggt ggatcagttc tggtttggtc tgaaagggat ggagcgttac
    93781 ggttatcgcg atgatgccct gaaactggcg gatacgttct tccggcacgc caaagggtta
    93841 accgccgatg gcccaattca ggaaaattac aacccgctga caggcgcaca gcaaggcgca
    93901 ccaaatttct cctggagtgc cgcacatttt tatatgttgt ataacgattt tttccgtaag
    93961 caataatttg caacggctgg cggatgcggc gtgaacgcct tatccgccct acatattcgt
    94021 tcccgtaggc tggataagac gcgacaagcg tcgcatccgg catctgcacc tcgccaccgg
    94081 cggatgctgc gtgaacgcct tatccgccct acatgttcgt tcccgtaggc tggataagac
    94141 gcgacaagcg tcgcatccgg catctgcacc tcgccactgg cggatgcggc gtgaacgcct
    94201 tatccgccct acatgttcgt tcccgtaggc tggataagac ccgacaagcg tcgcatccgg
    94261 cgtctgcacc gcgccactgg cggatgcggc gtgaacgcct tatccgccct acatgtgcaa
    94321 tcccccaggt cggatagggc ccggcaagcg tcgcatccgg cgttaccacc gggcgtattc
    94381 tttttgaatc ccatcacaaa ccccgcactc cccttttccc ttttctccgg cgacggctaa
    94441 attagaactc atccgaccac ataacaatta ttttacatac tggacaattt tatgagctac
    94501 ccgtcgctgt tcgccccgct ggatttaggt tttaccacgt taaaaaaccg cgtgttgatg
    94561 ggttcaatgc acaccgggct ggaggaatac ccggacggtg ctgaacggct ggcagcgttt
    94621 tatgccgaac gcgcccgtca cggcgtggcg ctgattgtta gcggcggcat tgcaccagat
    94681 ttaacaggcg ttggcatgga aggcggcgca atgctcaatg acgccagcca gatcccacac
    94741 catcgcacca ttaccgaagc ggtacatcag gaaggcggca aaatagccct gcaaattttg
    94801 cataccgggc gctacagcta ccaaccgcat ctggtcgcac cgtccgcatt gcaggccccc
    94861 atcaaccgtt tcgttcctca tgaactcacc catgaagaga tcctgcaact gatcgacgat
    94921 ttcgcccgct gcgcgcaact ggcacgggag gcaggatacg acggtgtaga ggtaatgggt
    94981 tccgaagggt atttgatcaa cgaatttctg acgctgcgca ccaatcagcg cagtgaccag
    95041 tggggcggcg attaccacaa ccggatgcgg tttgccgttg aagtggtgag tgcggtacgc
    95101 gaacgcgttg gcaacgactt cattattatc taccgactgt cgatgctcga cctggtcgaa
    95161 ggcggcggga cttttgccga aacggtagag ctggcgcagg ccattgaagc ggcgggcgcg
    95221 accattatca acaccggcat tggctggcat gaagcgcgta ttccgaccat cgccacgccc
    95281 gtgccacgcg gcgcatttag ctgggtcacg cgcaaactga aaggtcacgt ctcgctgccg
    95341 ctggtgacca ccaaccggat taacgatccg caggttgccg acgatattct ctcgcgcggc
    95401 gatgccgata tggtatcgat ggcgcgaccg tttcttgcgg atgcggaact gctgtcaaaa
    95461 gcgcaatcag ggcgagccga tgagatcaac acctgcattg gctgcaacca ggcgtgtctc
    95521 gatcagatct tcgttggcaa ggtcacctcg tgtctggtga atcctcgcgc ctgccatgaa
    95581 accaaaatgc cgatccttcc cgccatgcag aaaaaaaatc tggcggtggt cggtgcggga
    95641 cctgctgggc tggcgtttgc cattaacgcg gcggcgcgtg gtcatcaggt aacgttgttt
    95701 gacgcgcaca gtgagatcgg cgggcagttt aatatcgcca aacagatccc cggcaaagag
    95761 gagttttacg aaacgctgcg ctattaccgc cggatgatcg aagtgacggg cgtgacgcta
    95821 aaactcaatc acaccgtgac ggcggatcag ttacaggcgt tcgatgaaac gatcctcgcc
    95881 agtgggatcg tgccgcgcac tccgcccatc gacgggatcg atcatccgaa ggtattgagt
    95941 tatctcgatg tactgcgcga caaagcgccg gtgggcaaca aagttgccat catcggttgt
    96001 ggcgggattg gttttgatac ggcgatgtat ttaagtcagc cgggcgaatc caccagccag
    96061 aatatcgccg ggttctgtaa tgaatggggg atcgacagca gcctacaaca ggctggtggc
    96121 ttaagcccgc agggaatgca gatcccgcgt agcccacggc agattgtgat gctccagcgc
    96181 aaagccagca aaccgggaca ggggttaggc aaaaccaccg gctggatcca tcgcaccacc
    96241 ctgctctcgc ggggtgtgaa aatgatccca ggcgtaagtt atcagaagat tgacgatgac
    96301 gggctgcatg tggtgatcaa cggcgaaacg caggtattag cagtggacaa tgtggtgatc
    96361 tgcgcagggc aagagccaaa ccgcgcattg gcgcagccgc tgattgatag cgggaaaacg
    96421 gtgcatttaa ttggcggctg cgatgtggct atggagctgg acgcacgacg ggcaattgcc
    96481 cagggaacac ggctggcgct ggagatttaa atcgt
//
LOCUS       DAZKIP010000006        83740 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZKIP010000006 DAZKIP010000000
VERSION     DAZKIP010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 83740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 83740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..83740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.006"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            148..591
                     /gene="cmtB"
                     /locus_tag="ACHOUK_000477"
     CDS             148..591
                     /gene="cmtB"
                     /locus_tag="ACHOUK_000477"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFP9895995.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            619..2007
                     /gene="cmtA"
                     /locus_tag="ACHOUK_000478"
     CDS             619..2007
                     /gene="cmtA"
                     /locus_tag="ACHOUK_000478"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFP9895996.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            2022..3299
                     /gene="yggP"
                     /locus_tag="ACHOUK_000479"
     CDS             2022..3299
                     /gene="yggP"
                     /locus_tag="ACHOUK_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFP9895997.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVELAASKGIELVYVNTKGMSDPVQMLRALTGDAGFDDIFVYAAVSAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            3296..4261
                     /gene="glpX"
                     /locus_tag="ACHOUK_000480"
     CDS             3296..4261
                     /gene="glpX"
                     /locus_tag="ACHOUK_000480"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFP9895998.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRDVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGNMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDVLVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLY"
     gene            4283..4792
                     /gene="fumE"
                     /locus_tag="ACHOUK_000481"
     CDS             4283..4792
                     /gene="fumE"
                     /locus_tag="ACHOUK_000481"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFP9895999.1"
                     /translation="MATLSEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEESVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLKEQD
                     ETLGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            4789..5502
                     /locus_tag="ACHOUK_000482"
     CDS             4789..5502
                     /locus_tag="ACHOUK_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFP9896000.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWAYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPIIIVEGNWLLLDDDN
                     WRQLAQFCDFSIFINAPATALRERLVGRKLAGGLSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(5474..6151)
                     /locus_tag="ACHOUK_000483"
     CDS             complement(5474..6151)
                     /locus_tag="ACHOUK_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9896001.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKIGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTASAHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNIN
                     PPDDIHP"
     gene            complement(6145..6822)
                     /locus_tag="ACHOUK_000484"
     CDS             complement(6145..6822)
                     /locus_tag="ACHOUK_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9896002.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(6810..7517)
                     /locus_tag="ACHOUK_000485"
     CDS             complement(6810..7517)
                     /locus_tag="ACHOUK_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFP9896003.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALTIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(7518..8096)
                     /locus_tag="ACHOUK_000486"
     CDS             complement(7518..8096)
                     /locus_tag="ACHOUK_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFP9896004.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKTLTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(8119..8550)
                     /locus_tag="ACHOUK_000487"
     CDS             complement(8119..8550)
                     /locus_tag="ACHOUK_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFP9896005.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQQQLHAAWIA
                     GCTGSLTDIALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            8922..9941
                     /gene="epd"
                     /locus_tag="ACHOUK_000488"
     CDS             8922..9941
                     /gene="epd"
                     /locus_tag="ACHOUK_000488"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFP9896006.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            9991..11154
                     /gene="pgk"
                     /locus_tag="ACHOUK_000489"
     CDS             9991..11154
                     /gene="pgk"
                     /locus_tag="ACHOUK_000489"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFP9896007.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDEILSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            11369..12448
                     /gene="fbaA"
                     /locus_tag="ACHOUK_000490"
     CDS             11369..12448
                     /gene="fbaA"
                     /locus_tag="ACHOUK_000490"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFP9896008.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(12512..12610)
                     /locus_tag="ACHOUK_000491"
                     /pseudo
     CDS             complement(12512..12610)
                     /locus_tag="ACHOUK_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12806..13666
                     /gene="mscS"
                     /locus_tag="ACHOUK_000492"
     CDS             12806..13666
                     /gene="mscS"
                     /locus_tag="ACHOUK_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFP9896009.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            13805..14440
                     /gene="argO"
                     /locus_tag="ACHOUK_000493"
     CDS             13805..14440
                     /gene="argO"
                     /locus_tag="ACHOUK_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFP9896010.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            14533..15273
                     /gene="yggE"
                     /locus_tag="ACHOUK_000494"
     CDS             14533..15273
                     /gene="yggE"
                     /locus_tag="ACHOUK_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFP9896011.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            15440..16336
                     /gene="ygfI"
                     /locus_tag="ACHOUK_000495"
                     /pseudo
     CDS             15440..16336
                     /gene="ygfI"
                     /locus_tag="ACHOUK_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(16333..17811)
                     /gene="scpC"
                     /locus_tag="ACHOUK_000496"
     CDS             complement(16333..17811)
                     /gene="scpC"
                     /locus_tag="ACHOUK_000496"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFP9896012.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHNEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(17835..18620)
                     /gene="scpB"
                     /locus_tag="ACHOUK_000497"
     CDS             complement(17835..18620)
                     /gene="scpB"
                     /locus_tag="ACHOUK_000497"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFP9896013.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(18631..19626)
                     /gene="meaB"
                     /locus_tag="ACHOUK_000498"
     CDS             complement(18631..19626)
                     /gene="meaB"
                     /locus_tag="ACHOUK_000498"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFP9896014.1"
                     /translation="MINEATLAESIRRLRQGEHATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(19619..21763)
                     /gene="scpA"
                     /locus_tag="ACHOUK_000499"
     CDS             complement(19619..21763)
                     /gene="scpA"
                     /locus_tag="ACHOUK_000499"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFP9896015.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKTGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIIAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEAAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGLDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(21967..22860)
                     /gene="argP"
                     /locus_tag="ACHOUK_000500"
     CDS             complement(21967..22860)
                     /gene="argP"
                     /locus_tag="ACHOUK_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFP9896016.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            23243..23902
                     /gene="rpiA"
                     /locus_tag="ACHOUK_000501"
     CDS             23243..23902
                     /gene="rpiA"
                     /locus_tag="ACHOUK_000501"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFP9896017.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            24157..25389
                     /gene="serA"
                     /locus_tag="ACHOUK_000502"
     CDS             24157..25389
                     /gene="serA"
                     /locus_tag="ACHOUK_000502"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFP9896018.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            25618..25677
                     /gene="ibsC"
                     /locus_tag="ACHOUK_000503"
     CDS             25618..25677
                     /gene="ibsC"
                     /locus_tag="ACHOUK_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFP9896019.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            26011..26070
                     /locus_tag="ACHOUK_000504"
     CDS             26011..26070
                     /locus_tag="ACHOUK_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFP9896020.1"
                     /translation="MMRLVIILIVLLLISLPAY"
     gene            complement(26171..26773)
                     /gene="fau"
                     /locus_tag="ACHOUK_000505"
     CDS             complement(26171..26773)
                     /gene="fau"
                     /locus_tag="ACHOUK_000505"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFP9896021.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(26794..26977)
                     /gene="ssrS"
                     /locus_tag="ACHOUK_000506"
     ncRNA           complement(26794..26977)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHOUK_000506"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(27019..27348)
                     /gene="zapA"
                     /locus_tag="ACHOUK_000507"
     CDS             complement(27019..27348)
                     /gene="zapA"
                     /locus_tag="ACHOUK_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFP9896022.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            27516..28094
                     /gene="ygfB"
                     /locus_tag="ACHOUK_000508"
     CDS             27516..28094
                     /gene="ygfB"
                     /locus_tag="ACHOUK_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFP9896023.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            28120..29445
                     /gene="pepP"
                     /locus_tag="ACHOUK_000509"
     CDS             28120..29445
                     /gene="pepP"
                     /locus_tag="ACHOUK_000509"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFP9896024.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECEMRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DIDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            29442..30620
                     /gene="ubiH"
                     /locus_tag="ACHOUK_000510"
                     /gene_synonym="visB"
     CDS             29442..30620
                     /gene="ubiH"
                     /locus_tag="ACHOUK_000510"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFP9896025.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLEGGETLTGRVLVA
                     ADGTHSALATVCGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTHAARS
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWTPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            30644..31846
                     /gene="ubiI"
                     /locus_tag="ACHOUK_000511"
                     /gene_synonym="visC"
     CDS             30644..31846
                     /gene="ubiI"
                     /locus_tag="ACHOUK_000511"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFP9896026.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPVKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(31920..32003)
                     /locus_tag="ACHOUK_000512"
     CDS             complement(31920..32003)
                     /locus_tag="ACHOUK_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9896027.1"
                     /translation="MSKNTKSKIRDIRKYNAKTEVKLVYFK"
     gene            32294..33388
                     /gene="gcvT"
                     /locus_tag="ACHOUK_000513"
     CDS             32294..33388
                     /gene="gcvT"
                     /locus_tag="ACHOUK_000513"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFP9896028.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHEVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEGGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPKGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            33412..33801
                     /gene="gcvH"
                     /locus_tag="ACHOUK_000514"
     CDS             33412..33801
                     /gene="gcvH"
                     /locus_tag="ACHOUK_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFP9896029.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            33919..36792
                     /gene="gcvP"
                     /locus_tag="ACHOUK_000515"
     CDS             33919..36792
                     /gene="gcvP"
                     /locus_tag="ACHOUK_000515"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFP9896030.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDIFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPIGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(36958..38391)
                     /gene="bglA"
                     /locus_tag="ACHOUK_000516"
     CDS             complement(36958..38391)
                     /gene="bglA"
                     /locus_tag="ACHOUK_000516"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFP9896031.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINLEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDSDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQKPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGENL"
     gene            38436..38747
                     /gene="yqfB"
                     /locus_tag="ACHOUK_000517"
     CDS             38436..38747
                     /gene="yqfB"
                     /locus_tag="ACHOUK_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFP9896032.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELKKVIADIYPGQTQFYVIEFKC
                     L"
     gene            38911..39570
                     /gene="yqfA"
                     /locus_tag="ACHOUK_000518"
     CDS             38911..39570
                     /gene="yqfA"
                     /locus_tag="ACHOUK_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFP9896033.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(39647..40627)
                     /gene="ygfZ"
                     /locus_tag="ACHOUK_000519"
     CDS             complement(39647..40627)
                     /gene="ygfZ"
                     /locus_tag="ACHOUK_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFP9896034.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYLQGQ
                     VTADVSQMTDDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLAELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLTGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLCIEPLPYSLEE"
     gene            40870..41136
                     /gene="sdhE"
                     /locus_tag="ACHOUK_000520"
     CDS             40870..41136
                     /gene="sdhE"
                     /locus_tag="ACHOUK_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFP9896035.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            41117..41524
                     /gene="ygfX"
                     /locus_tag="ACHOUK_000521"
     CDS             41117..41524
                     /gene="ygfX"
                     /locus_tag="ACHOUK_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFP9896036.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     SGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(41564..42085)
                     /gene="fldB"
                     /locus_tag="ACHOUK_000522"
     CDS             complement(41564..42085)
                     /gene="fldB"
                     /locus_tag="ACHOUK_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFP9896037.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            42197..43093
                     /gene="xerD"
                     /locus_tag="ACHOUK_000523"
     CDS             42197..43093
                     /gene="xerD"
                     /locus_tag="ACHOUK_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFP9896038.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            43118..43828
                     /gene="dsbC"
                     /locus_tag="ACHOUK_000524"
     CDS             43118..43828
                     /gene="dsbC"
                     /locus_tag="ACHOUK_000524"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFP9896039.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            43834..45567
                     /gene="recJ"
                     /locus_tag="ACHOUK_000525"
     CDS             43834..45567
                     /gene="recJ"
                     /locus_tag="ACHOUK_000525"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFP9896040.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQIEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            45658..46756
                     /gene="prfB"
                     /locus_tag="ACHOUK_000526"
     CDS             join(45658..45732,45734..46756)
                     /gene="prfB"
                     /locus_tag="ACHOUK_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFP9896041.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            46766..48283
                     /gene="lysS"
                     /locus_tag="ACHOUK_000527"
     CDS             46766..48283
                     /gene="lysS"
                     /locus_tag="ACHOUK_000527"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFP9896042.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIVESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(48326..48874)
                     /gene="idi"
                     /locus_tag="ACHOUK_000528"
     CDS             complement(48326..48874)
                     /gene="idi"
                     /locus_tag="ACHOUK_000528"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFP9896043.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            48929..49000
                     /locus_tag="ACHOUK_000529"
     CDS             48929..49000
                     /locus_tag="ACHOUK_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9896044.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(48997..49122)
                     /gene="yqfG"
                     /locus_tag="ACHOUK_000530"
     CDS             complement(48997..49122)
                     /gene="yqfG"
                     /locus_tag="ACHOUK_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFP9896045.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(49124..50572)
                     /gene="uacT"
                     /locus_tag="ACHOUK_000531"
     CDS             complement(49124..50572)
                     /gene="uacT"
                     /locus_tag="ACHOUK_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFP9896046.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAQLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            51008..52927
                     /gene="ygfT"
                     /locus_tag="ACHOUK_000532"
     CDS             51008..52927
                     /gene="ygfT"
                     /locus_tag="ACHOUK_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFP9896047.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGLAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKNHFGEIYCGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALEMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            52927..53415
                     /gene="ygfS"
                     /locus_tag="ACHOUK_000533"
                     /pseudo
     CDS             52927..53415
                     /gene="ygfS"
                     /locus_tag="ACHOUK_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
     gene            complement(53451..54818)
                     /gene="ghxQ"
                     /locus_tag="ACHOUK_000534"
                     /gene_synonym="ygfQ"
     CDS             complement(53451..54818)
                     /gene="ghxQ"
                     /locus_tag="ACHOUK_000534"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HFP9896048.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(54854..56170)
                     /gene="guaD"
                     /locus_tag="ACHOUK_000535"
     CDS             complement(54854..56170)
                     /gene="guaD"
                     /locus_tag="ACHOUK_000535"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HFP9896049.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLKEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(56188..57588)
                     /gene="xanQ"
                     /locus_tag="ACHOUK_000536"
     CDS             complement(56188..57588)
                     /gene="xanQ"
                     /locus_tag="ACHOUK_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HFP9896050.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGALLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(57753..60623)
                     /gene="xdhD"
                     /locus_tag="ACHOUK_000537"
     CDS             complement(57753..60623)
                     /gene="xdhD"
                     /locus_tag="ACHOUK_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFP9896051.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIEVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVDMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(60620..61399)
                     /gene="ygfM"
                     /locus_tag="ACHOUK_000538"
     CDS             complement(60620..61399)
                     /gene="ygfM"
                     /locus_tag="ACHOUK_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFP9896052.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(61449..62777)
                     /gene="ssnA"
                     /locus_tag="ACHOUK_000539"
     CDS             complement(61449..62777)
                     /gene="ssnA"
                     /locus_tag="ACHOUK_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFP9896053.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPSPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVN
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(62780..65878)
                     /gene="ygfK"
                     /locus_tag="ACHOUK_000540"
     CDS             complement(62780..65878)
                     /gene="ygfK"
                     /locus_tag="ACHOUK_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFP9896054.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVHGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQDFHYVLIATGTDKNSGVKLAGDNQNVLKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATVVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(66200..66778)
                     /gene="mocA"
                     /locus_tag="ACHOUK_000541"
     CDS             complement(66200..66778)
                     /gene="mocA"
                     /locus_tag="ACHOUK_000541"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFP9896055.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIKRPNITNMRQALLMG
                     AHYSVEVENAEIILDIDTPDDFITAQKRYTKI"
     gene            66881..67651
                     /gene="yqeC"
                     /locus_tag="ACHOUK_000542"
     CDS             66881..67651
                     /gene="yqeC"
                     /locus_tag="ACHOUK_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFP9896056.1"
                     /translation="MKSIVDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANLGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRAMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            67699..69324
                     /gene="yqeB"
                     /locus_tag="ACHOUK_000543"
     CDS             67699..69324
                     /gene="yqeB"
                     /locus_tag="ACHOUK_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFP9896057.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIIDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFA
                     AGKDCHAVIETNRGHWLGQVIYCGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(69365..70297)
                     /gene="arcC"
                     /locus_tag="ACHOUK_000544"
     CDS             complement(69365..70297)
                     /gene="arcC"
                     /locus_tag="ACHOUK_000544"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFP9896058.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVVTLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVEAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(70345..71730)
                     /gene="hyuA"
                     /locus_tag="ACHOUK_000545"
     CDS             complement(70345..71730)
                     /gene="hyuA"
                     /locus_tag="ACHOUK_000545"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFP9896059.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(71783..72994)
                     /gene="ygeY"
                     /locus_tag="ACHOUK_000546"
     CDS             complement(71783..72994)
                     /gene="ygeY"
                     /locus_tag="ACHOUK_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFP9896060.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(73052..74248)
                     /gene="dpaL"
                     /locus_tag="ACHOUK_000547"
     CDS             complement(73052..74248)
                     /gene="dpaL"
                     /locus_tag="ACHOUK_000547"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFP9896061.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(74306..75493)
                     /gene="ygeW"
                     /locus_tag="ACHOUK_000548"
     CDS             complement(74306..75493)
                     /gene="ygeW"
                     /locus_tag="ACHOUK_000548"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFP9896062.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEQRTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            75972..77750
                     /gene="ygeV"
                     /locus_tag="ACHOUK_000549"
     CDS             75972..77750
                     /gene="ygeV"
                     /locus_tag="ACHOUK_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP9896063.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLYRVAG
                     TGAYGKFLGRQLSGNSRLLCHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVTEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(77790..78269)
                     /gene="xdhC"
                     /locus_tag="ACHOUK_000550"
     CDS             complement(77790..78269)
                     /gene="xdhC"
                     /locus_tag="ACHOUK_000550"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFP9896064.1"
                     /translation="MNHSETITIECTINGMPFQLHAVPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(78266..79144)
                     /gene="xdhB"
                     /locus_tag="ACHOUK_000551"
     CDS             complement(78266..79144)
                     /gene="xdhB"
                     /locus_tag="ACHOUK_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFP9896065.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDSITQRHLPALCAAASSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHVGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(79155..81452)
                     /gene="xdhA"
                     /locus_tag="ACHOUK_000552"
     CDS             complement(79155..81452)
                     /gene="xdhA"
                     /locus_tag="ACHOUK_000552"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFP9896066.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWEELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYVSHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNASREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            81868..82623
                     /gene="actS"
                     /locus_tag="ACHOUK_000553"
     CDS             81868..82623
                     /gene="actS"
                     /locus_tag="ACHOUK_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFP9896067.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            82702..82775
                     /locus_tag="ACHOUK_000554"
     tRNA            82702..82775
                     /locus_tag="ACHOUK_000554"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:82734..82736,aa:Gly,seq:ccc)
     gene            83009..83740
                     /gene="ygeQ"
                     /locus_tag="ACHOUK_000555"
                     /pseudo
     CDS             83009..83740
                     /gene="ygeQ"
                     /locus_tag="ACHOUK_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgeQ"
BASE COUNT        20339 a        20791 c        22080 g        20530 t
ORIGIN      
        1 tatatctata actcaatgtt atataagaaa ttaataattc actgttttca aaacaccggt
       61 ttccctgctc aattgctttc attaaaccgc acgagtattt tatttttacc gccggacggt
      121 accctaaaaa taaaataagg ataatttatg cggcttagtg actattttcc agaatcatca
      181 atctcagtta tacattcagc aaaagattgg caggaagcta tcgatttctc gatggtatca
      241 ttgctggata aaaactatat cagcgagaat tacattcagg caattaaaga ttccaccatt
      301 aacaatggcc cttattatat tctcgcacca ggcgtggcaa tgcctcatgc gcgaccggaa
      361 tgtggggcgc ttaaaaccgg gatgtctttg acattacttg aacaaggtgt ttattttccg
      421 gggaatgacg aaccaattaa attactcatc ggactctctg ctgccgatgc cgattcgcac
      481 attggcgcta ttcaggcgtt aagtgaatta ctgtgcgaag aagaaatact cgaacaactc
      541 ttaacagcat catcagaaaa acaattagcg gacattatca gccgcggata attactctct
      601 cctctacagg ataatattat ggaaaacaag tctgctcgtg caaaggtcca ggcttttggg
      661 ggctttttga ctgcaatggt catccccaat attggtgctt ttattgcctg gggttttatt
      721 actgcgttat ttattcccac cggttggctg cctaacgaac atttcgccaa aattgtcggc
      781 ccgatgatta cctatttatt gcccgtgatg attggttcta ccggtggtca tctggtcggc
      841 ggtaaacgcg gcgcggtcat gggcggaata ggtactattg gtgtgatcgt cggcgcagag
      901 atcccgatgt tccttggctc aatgattatg gggccgctcg gtggtctggt cataaaatat
      961 gttgataagg cactggaaaa acgcatacct gccggttttg agatggttat caataacttc
     1021 tcattaggta tcgcggggat gctcctttgc ctgctgggtt ttgaagtgat cggcccggcg
     1081 gtgttaattg ccaatacttt cgtcaaagag tgtattgagg cgctggtaca tgcgggttat
     1141 ctgcctctgc tgtcagtcat caatgaaccg gcgaaagtgc ttttccttaa taatgcgatc
     1201 gatcagggcg tctattatcc gctgggaatg caacaggctt cggttaacgg taaatccatc
     1261 ttctttatgg tggcctctaa cccaggtccg ggcctggggc tgctgctggc gtttaccttg
     1321 tttggtaaag ggatgagtaa acgttctgcg cccggggcga tgattattca cttcctcggt
     1381 gggatccacg aactgtattt cccatatgtg ctgatgaagc cgctgactat tattgccatg
     1441 attgcgggcg gtatgtctgg cacctggatg tttaacttac tggacggtgg tctggtggct
     1501 ggcccaagtc cggggtctat ctttgcttac ctggcgctga cgccgaaagg ttcgttcctg
     1561 gcgacaattg ccggtgttac ggtaggtacc ctggtgtcct ttgctatcac ttcgctgata
     1621 ctgaagatgg aaaaaacggt ggaaacggag agcgaagatg agtttgctca gtcagccaat
     1681 gcggttaagg cgatgaaaca agagggtgca ttctcgttaa gcagggttaa gcgtatcgcc
     1741 tttgtttgcg atgcggggat gggctccagt gcgatgggcg cgaccacctt ccgtaaacgc
     1801 ctggaaaaag cggggctggc aattgaagta aaacattacg ccatagaaaa cgtgcctgcg
     1861 gatgcggata tcgtcgttac tcatgccagt ctggaagggc gcgtgaaacg tgtgacggat
     1921 aaaccactga tattgattaa taactatatt ggcgatccaa aactcgacac tttatttaat
     1981 caattaaccg ccgaacataa acactgatta cagaggtaaa aatgaaaacc aaagttgctg
     2041 ctatttatgg caagcgggat gtccgtctgc gcgaatttga actgccagaa attaccgata
     2101 atgaattact ggtgagtgtg atttctgaca gcgtctgttt atccacctgg aaagcggcgt
     2161 tactcggtag tgaacataaa cgcgtacccg acgatttaga aaatcatccg gtcattaccg
     2221 ggcatgaatg tgccggggtt attgtcgaag tgggtaaaaa tctcactggc aaatataaaa
     2281 aaggccagcg ttttgtattg caaccggcga tggggttacc aagcggatat tcagcgggct
     2341 acagctacga atattttggc ggcaatgcca cttatatgat tattcccgaa atcgccatta
     2401 atttgggctg cgtattaccg tatcacggct cttattttgc tgcggcgtcg ctggcagagc
     2461 ctatgtgctg cattattggc gcttatcatg ccaattatca caccacgcaa tatgtttatg
     2521 agcatcgcat gggcgtcaaa cctggcggca atattgcact gctggcgtgt gcaggtccga
     2581 tgggcattgg cgctatcgat tacgccatta acggcggcat acaaccgtcg cgggtggtgg
     2641 tggtcgatat cgacgacaaa cgtctggcgc aggtacagaa gctgctgccg gtggaactgg
     2701 cggccagtaa aggcattgag ctggtgtatg tgaataccaa agggatgagc gatcctgtcc
     2761 agatgctgcg ggcgctgaca ggagatgccg ggttcgatga catttttgtt tatgcggcgg
     2821 tgtctgctgt cgttgagatg gctgatgaat tactggcgga agatggctgt ctgaacttct
     2881 ttgccgggcc gacggataaa aacttcaaag tgccgtttaa tttctacaac gttcattaca
     2941 acagcacgca cgtcgtcggt acatccggcg gttcaacgga cgacatgaaa gaggcgattg
     3001 cccttagcgc cacagggcag ttacagccct cgtttatggt gacccatatc ggtggcctgg
     3061 atgcggtgcc agaaaccgtg ctcaatctgc cggatatccc tggcggtaaa aaactcattt
     3121 ataacggcgt gaccatgccg ctcactgcca ttgccgattt tgccgaaaaa ggcaaaaccg
     3181 atccgctgtt taaagagttg gcgcggctgg ttgaggaaac gcacggcatc tggaatgaac
     3241 aggccgagaa atatctgctg gcacaatttg gcgttgatat cggggaggcc gcgcaatgat
     3301 gtccctggcg tggccattat ttcgcgttac ggaacaggca gcgttggctg cctggccgca
     3361 aaccggatgt ggcgacaaaa ataaaattga tggcctggcg gtcaccgcga tgcgccaggc
     3421 attaaacgac gtcgcttttc gtggacgagt ggttatcggc gaaggagaaa ttgaccatgc
     3481 gccgatgctg tggattggcg aagaggtggg caaaggcgat gggccagaag tcgatatcgc
     3541 ggttgatccc attgaaggca cgcggatggt ggcgatgggg cagagcaatg cgctggcggt
     3601 catggctttc gcccctcgag atagcctgtt acatgcgccc gatatgtata tgaaaaagct
     3661 ggttgttaat cgactggctg ccggggcgat tgatctgtct ttgcctctgg cggacaactt
     3721 gcgtgacgtg gcgcgggcgt taggtaaacc gctggacaag ctgcgcatgg ttacgctgga
     3781 taaaccgcgt ctgagtgccg cgattgaaga agcaacacag ctgggcgtga aggtttttgc
     3841 cctgccggat ggcgatgtcg ccgccagcgt gctgacctgc tggcaggata atccttacga
     3901 tgtgatgtac accatcggcg gtgcgccgga aggcgtgatt tctgcctgtg ccgttaaagc
     3961 attaggcggg aatatgcagg ccgagctgat tgacttttgt caggccaaag gggattacac
     4021 ggaaaatcgg cagattgcag agcaggagcg caagcgttgc aaggcaatgg gtgttgacgt
     4081 taaccgtgtg tactcgctcg atgtactggt aagggggaac gatatcctct ttagcgctac
     4141 gggcgtcacg ggcggcgagt tggtgaacgg tatccaacag acggcgaatg gagtgcggac
     4201 gcagacatta ctgatcggcg gcgcggacca aacgtgtaat ataatagact ccctgtattg
     4261 atggtgatct atcggaagac caatggcgac gctgtcagaa gatgatgtgc ttgagcaact
     4321 ggatgcacag gacaatttat tttcatttat gaaaactgcg cattctattt tgctccaggg
     4381 gatacgccag tttctgccgt cgctgtttgt cgataacgat gaagagagcg tcgaatatgc
     4441 agtgaagccg ttactcgccc aaagcggccc gcttgacgat attgatgttg cactgcgttt
     4501 gatttatgcg ctggggaaaa tggataaatg gctgtacgcc gatatcacgc atttttccca
     4561 gtactggcat tacctgaaag aacaggatga aacgctcgga tttgccgatg acatgacttg
     4621 ggattttatc agcaacgtca atagcataac ctgcaatgcg acgctctatg acgcgttgaa
     4681 agcgatgaag ttcgccgatt tcgccgtctg gtcagaggcg cgttttagcg gaatggtcaa
     4741 aacggcgctg acgctggcag taacgaccac cttaaaggaa ttaacgccgt gaaaattgaa
     4801 ttaacggtga atgggctgaa cgtgcaagcg cagtaccatg atgaagagat agaacgcgtg
     4861 cataaaccgt tgctgcgtat gctggcggcc ttacagacgg taaatccgca gcgccgaacc
     4921 gtggtttttc tttgcgctcc gccggggacg ggtaaatcta ccctaaccac cttctgggca
     4981 tatctcgcgc aacaagaccc ggagctgccc gctattcaga cgctcccgat ggatggtttt
     5041 caccattaca atagctggct ggatgcgcat caattgcgcc ccttcaaagg cgcaccagag
     5101 acatttgacg ttgcgaaact ggcggaaaat ctgcgccagg tcgtggaagg ggattgtacg
     5161 tggccgcagt acgatcgaca aaagcatgat cctgttgaag atgcgttgca cgttaccgca
     5221 ccaataatca tcgtcgaagg aaactggcta ttgctggatg acgataactg gcgccagcta
     5281 gcgcaatttt gtgatttctc gatctttatc aatgcgccag ctactgcgtt gcgggaacga
     5341 ctggtggggc gcaagctggc gggagggtta tcgctggcag acgcagaagc tttttatgac
     5401 cgtaccgacg ggccgaatgt tcgccgggtg ctggaagaga gtctgcctgc aaatttgacc
     5461 ttaatgatga ccgctacggg tgaatatcgt ctggtggatt aatgttcctg attagaccat
     5521 cttccagtcg caccacacga gagaaatgac gccgggtaaa tgcggcgtca tgactgattg
     5581 ctacgacgct cgtgcctcgc tggcggcact tctccagcca gctttcaaac acgctcaacc
     5641 aattttcgtc aaaatcccga ctcggctcgt ccagcagcaa tagtggcgga gagagtgctt
     5701 ccaggcaggc gacggcgacc atccgtcgct gcgcactatg cagatccaac gggtgcgcgc
     5761 tggcagtgtc tgctaactga caacattgca gggcagcatg ggtacgttga gtgatttcat
     5821 ccgccgggca tttttgcagt ttcaaaccaa atgcgatctc gtcagcgacg gtgctgtgaa
     5881 aaagttggtt ttccgcttcc tgaaacagta cgccaatctt tgccgcgcgc tggcggtttt
     5941 tcaggttttt catcgcctgt tgttgcagca taaccgtacc ggctgtgggc gtaagaagac
     6001 ctgccattac tcgtaacagc gtcgatttcc ctgcgccatt gtcgccggtc aacgccaacc
     6061 attcaccttg tttgagttgc agagaaatgt cgcaaagaca atccgttgcc gcgccaggcc
     6121 agcgataaga tatctgattt aacgttaaca tagtggctgg agcgctccgt tttgcaattg
     6181 ccacgcttgc tggcagtagt tcaggaaagg tgtgcgatga cgttcaaaaa gaataatcag
     6241 cgaaccacgt tcaaacgccc agtgttgtaa gcgctgcaac agcatttcgc tggcctgcgg
     6301 cgtcaggcgg ctaaaggctt catcaagaat taatagcttt ggctgcatcg cgatggcgca
     6361 ggcgatgacc acgcgctgtg tctctccacc ggagagtgtt gccgggtgac gatggcgcaa
     6421 cggctggcac tcggtcagag tcagggcggc atcaatccgc gccataatct ctgcttcagc
     6481 aagacacaga ttttctggcc caaaggcaac ttcttcttcc acgctaaagg tacagccgga
     6541 gagctgcaaa tagggcgatt gctggacgag ctggatggtg gcggattgtt cattaagcgg
     6601 gagatgccca atgggcgtac cgaggagcgt ccccgtgcct gttatctctc ctggcagaaa
     6661 gtccggatac cagcccgcca tcagctgtgc cagcgtactt ttgccactgc cgttgtcacc
     6721 aaagatggcg accatccccg gtttgacata gtgaaagtca taacagaacg gaggatgcgt
     6781 tgagtgtgtc gggcaataac ggaactgttc taacgtaacc atatccacgc tccgaattct
     6841 gtcagcatca gcaagatcat ggtgtaacgc gctacgcgtt gtagggtgct atcggctggt
     6901 gcccacaacg ttgttcgtcg gttgtggatc cgaaatgccc gcatatccag cgccgcacca
     6961 cggacagcaa gatcgtttag cgcattgtgt gttagcggaa tagttaacgc gggcatcgca
     7021 cgtaaacgct gataccagcc ctcatccagc ggcacgccgc gggcacgctg cgcttcatga
     7081 ataatcgcta attgccgttt taactgctca acgaccaata acggcccggc aaacagataa
     7141 gcgacgcccg gcggcaggcg agaagcaaac agagcgcgga taaaacattg cacggggacg
     7201 tactgcatcc acaactgtga ggttgaaaca atcgccagaa tccgcaacca gagcgtaata
     7261 gcatgcgtcc aacgctccgg cgaacgcggc gtgccgctta gccattctgt tagccagcca
     7321 ccgtgtacca gccacagccc ggctcccagc gagaacatca gccagacgac atattttgcc
     7381 cgccgccgcg tggcctttag cgcaataagg caaaaaaagg tcgccgcgct atagatcggc
     7441 aaaatggtct gcgcgggcag gataagcgtg gtgcaggccg ccaatgccca tagtgttaat
     7501 gaggtaaatg gatgcattta gcgcacggca gccattgccg gaaaatggcg cgtggtgcga
     7561 atcggcagct ggcgcagtaa aagccagaca atcaccgctg taagagtttt atcgaccagg
     7621 ttcgcgccta tcaccgtaat cgccaccgac tccaccaggt tttgccccat cgagtgcatc
     7681 caggcaacga acaaatcggc tccgctaccg gtgacaccgc cgaacagcgc agtacgcagc
     7741 ggtacagcga cgaccgttac cgcaagcgta ataatcacgc cgctgacaac gacttttggc
     7801 agggtgcgaa accaacccgc tcgcgccagc caaccggcga ccaggccgat gaccatcgcg
     7861 acgggggcaa aagcggcagc gatcgggtcg gtcagtaatc cccagagtaa attggtcagt
     7921 aagccggtca gcatcccgat caccggacca agcagtaccg cgctaataag tgtgccgatg
     7981 gagtcgagaa atataggtaa tttgaccatg ctggccagtt gcccgccgat catattgatg
     8041 gcgatagata taacgatcaa caccagtgcc tgactggaaa agtggctacg cgccataaga
     8101 aaacctctct gaaagtagtt atttaacggg ggaaatatgc aactcgtcgt aaccagaaag
     8161 cgcgcaaggt tggcggctaa acgccagttc ctcaaggcta ccgatcacca gttcaagcgt
     8221 ggtccgcacg gtacagccgg gcatcatatg accgccaagc atcgtgccgt gtggatcgga
     8281 aacgcagaga tgaagatgct caccactctg ttccaacgtg ccgtttagtg agatcacttc
     8341 gaatgtaccg ttcaggagcg tggtgccttc ttgcccggca tagcgcaagg cgatgtcagt
     8401 caggctaccg gtacagcctg caatccaggc ggcatgaagt tgctgctgct gtgcaaaagc
     8461 gtgtagctgg gaaaaaactt cctgcccggg gaggaggcgc agtgcgtaaa agcgtgcgga
     8521 ggaagaatat gggtagggca atgtcatcat tggagtactc cgaaactcgg acgtattacg
     8581 gataatatcg acgctgcatc agtcatattc tgatataagt cagggtttgt gacggcacaa
     8641 cgaaaagatt ttgctacgct tattcgtgat tctgcaaagt ggaataaatt ccacaggggc
     8701 ggggctgaaa acgtgacgta agtcagataa ttgttttctt tgactggaca atcattcctt
     8761 ttattccacg tttcgcttat cctagctgaa gcgtttcagt cgattaaatg ttcgacaatt
     8821 aaccaatcag tcgcagtttg cgacaggtaa ggtttccccg gacgatttgc tggattactc
     8881 tgtctggcaa atttgctcag ggaaaacctt gcaggagatc tatgaccgta cgcgtagcga
     8941 taaatggctt cggtcgcatc gggcgtaatg tggttcgtgc tttgtatgaa tccggacgcc
     9001 gggcggaaat taccgtggtg gcaatcaacg aactggcgga tgctgcgggc atggcgcatt
     9061 tgttgaaata cgacaccagc catggccgtt ttgcatggga agtacgacag gaacgcgatc
     9121 aactttttgt tggtgatgac gccatccgcg tattgcatga acgttcactg caatcgctcc
     9181 cctggcgtga acttggcgtt gatgtagtcc tcgactgcac cggcgtatat ggctcccgcg
     9241 agcatggcga agcgcacatt gccgccgggg ctaaaaaagt gctcttttca catcctggca
     9301 gtaacgatct cgacgcgacc gttgtttacg gcgtcaatca ggatcaactt cgtgcggagc
     9361 accgcatcgt ttctaacgct tcctgtacca cgaattgcat aattcccgtc atcaaattgt
     9421 tagatgatgc gtacggtatt gagtccggca ctgtgaccac aattcactcc gccatgcacg
     9481 atcaacaggt tattgatgca taccatcctg acctgcgtcg cacccgggca gccagccagt
     9541 cgatcattcc ggtcgatact aaactggccg ccggtatcac acgatttttt ccgcaattta
     9601 acgatcgctt tgaagcgatt gcggtacgtg tgccaaccat aaatgtgacg gcaatcgatt
     9661 taagcgtgac ggtgaagaaa cctgtaaaag ccaatgaagt caacctgttg ctgcaaaaag
     9721 cagcacaagg tgcatttcat ggtatagttg actatacgga attgccgttg gtctctgtag
     9781 attttaacca cgatccgcac agtgccattg tcgatggcac ccaaacccgg gtcagtggcg
     9841 cacacctgat caaaacgttg gtctggtgcg ataacgaatg gggctttgct aaccgaatgc
     9901 tcgacacgac gttagctatg gctactgttg ctttcaggta agacgcaagc agcgtctgca
     9961 aaacttttag aatcaacgag aggattcacc atgtctgtaa ttaagatgac cgatctggat
    10021 cttgctggga aacgtgtatt tatccgtgcg gatctgaacg taccagtaaa agacgggaaa
    10081 gtaaccagcg acgcgcgtat ccgtgcttct ctgccgacca tcgaactggc cctgaaacaa
    10141 ggcgcaaaag tgatggtaac ttcccacctg ggtcgtccta ccgaaggcga gtacaacgaa
    10201 gaattctctc tgctgccggt tgttaactac ctgaaagaca aactgtctaa cccggttcgt
    10261 ctggttaaag attacctcga cggcgttgac gttgctgaag gtgaactggt tgttctggaa
    10321 aacgttcgct tcaacaaagg cgagaagaaa gacgacgaaa tcctgtccaa aaaatacgct
    10381 gcactgtgtg acgtgttcgt aatggacgca ttcggtactg ctcaccgcgc gcaggcttct
    10441 actcacggta tcggtaaatt cgctgacgtt gcgtgcgcag gcccgctgct ggcagctgaa
    10501 ctggacgcgc tgggtaaggc actgaaagaa cctgctcgcc cgatggtggc tatcgttggt
    10561 ggttctaaag tatctaccaa actgaccgtt ctggactccc tgtctaaaat cgctgaccag
    10621 ctgattgttg gtggtggtat cgctaacacc tttatcgcgg cacaaggcca cgatgtgggt
    10681 aaatccctgt acgaagctga cctggttgac gaagcaaaac gtctgctgac cacctgcaac
    10741 atcccggttc cgtctgatgt tcgcgtagca accgagttct ctgaaaccgc tccggctacc
    10801 ctgaaatctg ttaacgacgt gaaagctgac gagcagatcc tggatatcgg tgatgcttcc
    10861 gctcaggaac tggctgaaat cctgaagaat gcgaaaacca ttctgtggaa cggtccggtt
    10921 ggcgtgttcg aattcccgaa cttccgcaaa ggtactgaaa tcgtggctaa cgctatcgca
    10981 gacagcgaag cgttctccat cgctggcggc ggcgacactc tggcagcaat cgacctgttc
    11041 ggcattgctg acaaaatctc ctacatctcc actggcggcg gcgcattcct cgaattcgtg
    11101 gaaggtaaag tactgcctgc agtagcgatg ctcgaagagc gcgctaagaa gtaaaaaatc
    11161 acagggcagg gaaacctgcc cttgtttcag cgcgctttta gagcacgcat tttttcatac
    11221 tctaaataat tcgagttgca ggaaggcgac aagagagtga atccccagga gcttacataa
    11281 gtaagtgact ggggtgagcg aacgcagacg cagcacatgc aacttgaagt atgacgagta
    11341 taaggcccga cgatacagga caagagacat gtctaagatt tttgatttcg taaaacctgg
    11401 cgtaatcact ggtgatgacg tacagaaagt tttccaggta gcaaaagaaa acaacttcgc
    11461 actgccagca gtaaactgcg tcggtactga ctccatcaac gccgtactgg aaactgctgc
    11521 taaagttaaa gcgccggtta tcgttcagtt ctccaacggt ggtgcttcct ttatcgctgg
    11581 taaaggcgtg aaatctgacg ttccgcaggg tgctgctatc ctgggcgcga tctctggtgc
    11641 gcatcacgtt caccagatgg ctgaacatta tggtgttccg gttatcctgc acactgacca
    11701 ctgcgcgaag aaactgctgc cgtggatcga cggtctgctg gacgcgggtg aaaaacactt
    11761 cgcagctacc ggtaagccgc tgttctcttc tcacatgatc gacctgtctg aagaatctct
    11821 gcaagagaac atcgaaatct gctctaaata cctggagcgc atgtccaaaa tcggcatgac
    11881 tctggaaatc gaactgggtt gcaccggtgg tgaagaagac ggcgtggaca acagccacat
    11941 ggacgcttct gcactgtaca cccagccgga agacgttgat tacgcataca ccgaactgag
    12001 caaaatcagc ccgcgtttca ccatcgcagc gtccttcggt aacgtacacg gtgtttacaa
    12061 gccgggtaac gtggttctga ctccgaccat cctgcgtgat tctcaggaat atgtttctaa
    12121 gaaacacaac ctgccgcaca acagcctgaa cttcgtattc cacggtggtt ccggttctac
    12181 tgctcaggaa atcaaagact ccgtaagcta cggcgtagtg aaaatgaaca tcgataccga
    12241 tacccaatgg gcaacctggg aaggcgttct gaactactac aaagcgaacg aagcttatct
    12301 gcagggtcag ctgggtaacc cgaaaggcga agatcagccg aacaagaaat actacgatcc
    12361 gcgcgtatgg ctgcgtgccg gtcagacttc gatgatcgct cgtctggaaa aagcattcca
    12421 ggaactgaac gcgatcgacg ttctgtaagt cattcctttc tgcttatctc aaggcccgct
    12481 ctgcgggtct ttttttcgcc aaaagcaaaa ttgcctgatg cgctatgctt atcaggccta
    12541 cacgtatcct gcaatatatt gaatttgcag gtttttgtag gccggataag gcgttcacgc
    12601 cgcatccggc atgacaaagc gcacattgat atctgtctga gccccgctct gcggggattt
    12661 ttttgccaaa agcaaggtga ttcagatgag aaatctgtga tctatttggc aaaattatgc
    12721 tttattgttt acccttgtca gactgcccgt cataaggcgg cggagtgtat ttctccattt
    12781 tgagtcagtt gaaaaggaat attgaatgga agatttgaat gttgtcgata gcataaacgg
    12841 cgcgggaagc tggctggtag ctaaccaggc gctgctgcta agttatgcag taaacatcgt
    12901 ggcggcactc gcgatcatca tcgttggttt gattatcgcg cggatgattt ccaacgcggt
    12961 gaatcgcctg atgatctccc gtaaaatcga tgccactgtt gctgattttc tttctgcatt
    13021 agtccgttac ggtattatcg cctttacgct aatcgcagcc ctgggacgcg tgggtgtaca
    13081 aacagcgtca gtcattgctg tactcggtgc cgcaggctta gctgttggtc tggctttgca
    13141 ggggtcactt tctaacctgg ccgctggcgt gttacttgtc atgttccgcc cgttccgtgc
    13201 cggagaatat gttgacctcg gcggcgtagc cggtactgtg ctgagtgtgc agattttctc
    13261 caccaccatg cgtactgcag acggtaaaat tatcgttatt ccgaacggta aaattattgc
    13321 cggaaatatt attaacttct cccgcgagcc agttcgccgt aacgaattta ttattggcgt
    13381 ggcgtatgat tccgatatcg atcaggttaa gcagatcctg accaatatta tccagtctga
    13441 agatcgcatt ttgaaagatc gcgaaatgac tgtgcgcctg aacgaacttg gtgcatcgtc
    13501 gattaatttc gtggtccgcg tctggagcaa cagcggcgat ctgcaaaacg tgtactggga
    13561 tgtgctggag cgtattaaac gtgaatttga tgccgccggt atcagcttcc cgtacccgca
    13621 aatggatgtg aactttaagc gggtgaaaga agacaaagct gcgtaatcaa cgctgaggcc
    13681 agataatact ctctggcctc tctcttatta gttattctga ttgccaatta atattatcaa
    13741 tttccgctaa taacaatccc gcgatatagt ctctgcatca gatacttaat tcggaatatc
    13801 caacgtgttt tcttattact ttcaaggtct tgctcttggg gcagctatga ttctgccgct
    13861 cggcccacaa aatgcttttg tgatgaatca gggcattcgt cgtcagtacc acattatgat
    13921 tgccttactt tgcgctatca gcgatttggt cctgatttgc gcggggattt ttggtggtag
    13981 cgcgttattg atgcagtcgc cgtggttgct ggcgctggtc acctggggcg gcgtagtctt
    14041 cttgctgtgg tatggttttg gcgcttttaa aacagcgatg agtagtaata ttgagttagc
    14101 tagtgccgaa gtcctgaagc aaggcagatg gaaaattatc gccaccatgt tggcagtgac
    14161 ctggctgaat ccgcatgttt acctagatac ttttgttgta ctgggcagcc ttggcgggca
    14221 gcttgacgtc gaaccaaaac gttggtttgc gctcgggaca attagcgcct ctttcctgtg
    14281 gttctttggt ctggctattc tcgcagcctg gctggcaccg cgcctgcgca cggcaaaatc
    14341 acagcgcatt atcaatctgg tagtgggatg tgtcatgtgg tttatagcct tgcagctggc
    14401 gagagacggt attgctcatg cacaagcctt gttcagttag gcgctatctg atggaaaaat
    14461 aaaacagagg cgctaagctt gcctccagag atcctgaatt ttgggcaagt caattaacac
    14521 ggagagacta acgtgaagtt caaagttatc gccctggcgg cattaatggg tattagcggg
    14581 atggcagcgc aggctaacga attgccggat ggaccgcata ttgtcacctc cggtacggca
    14641 agtgtggatg cggtgccaga cattgccact cttgcgattg aagttaacgt ggccgcgaag
    14701 gatgccgcta cagccaagaa acaggcagat gagcgcgtcg cacaatacat ttccttcctt
    14761 gaactcaatc agatcgcgaa aaaagatatc agctcagcga atttacgcac ccagccagat
    14821 tatgattatc aggatggtaa aagtatcctg aaaggctacc gcgctgtgag aacggtggaa
    14881 gtcacgctcc gtcagttaga caaactgaat tccttgctgg atggcgcgct gaaggcgggt
    14941 cttaacgaaa ttcgttcagt gtcgctgggc gtggcgcagc cggatgccta taaagacaaa
    15001 gcgcgtaagg cagcgattga taacgcgatt catcaggcgc aggaactggc gaacggcttt
    15061 catcgtaaac tggggccggt atatagcgtg cgctaccatg tttccaacta tcagcccagc
    15121 ccaatggtgc ggatgatgaa agccgatgcc gcgccggtgt ctgcccagga aacttacgag
    15181 caggccgcta ttcagtttga tgatcaggtc gatgtggtct tccagttaga acctgtggat
    15241 caacaacccg ctaaaacacc tgcagcacaa taatacttgt agcccgacgt attcgtcggg
    15301 ctaatgcctt tacccgatat tgcctccttt ttctgtaatt ttctgtaaaa ccatgacgta
    15361 atcacctatc tttgctcgcg gtttcatcaa ggataggctg actgaaacgg ttaagcggac
    15421 aaggatgcgg ggagggggga tggacatttt tatttcgaaa aagatgcgca atttcatttt
    15481 attagcgcaa accaataata ttgcccgggc ggcagaaaaa atccatatga cggcttcgcc
    15541 atttggtaaa agcattgccg cgctggaaga acaaattggc tatacgctat ttacccgcaa
    15601 agataataat atcagcctca ataaagccgg tcaggaactg tatcaaaaac tgttcccggt
    15661 ttatcaacga ctttctgcga ttgataatga aatccataac tcagggcgtc gttcacgaga
    15721 aattgtgatt ggcatagata atacttatcc gacaattatt ttcgatcagt tgattagtct
    15781 cggagataag tatgaaggcg tgaccgccca gccagttgag ttcagcgaaa acggcgttat
    15841 cgataatctt tttgaccgtc agttagattt tattatctcc ccacagcatg tatctgcacg
    15901 agttcaggaa ctggaaaacc tgactatcag tgaattacca ccgctgcgat tgaactttct
    15961 cgtttctcgc cgttatgaag agcgtcaaga gcaagagctg cttcaggagc tgccctagct
    16021 acaaatgcgc tttcaaaacc gggctaactt cgaagcaatg attgatgcga atatgcgacc
    16081 ttgtgggatc aatcccacca ttatttatcg tccctacagt tttatggcca aaatcagcgc
    16141 cgtggagcgc ggacattttc tgacggtgat cccgcatttt gcctggcgac tggtgaatcc
    16201 ggcaacgctg aaatattttg atgcgccgca caggccgatg tatatgcagg aatatcttta
    16261 ttcaatcaga aatcatcgtt atactgccac gatgcttcag catattgctg aagatcgtga
    16321 cgggacaaac cattaaccca gcatcgagcc ggttgcaatt aaattacggt gtaagtcgaa
    16381 gacgtggcta agatcgtggt gaatatgtcc gccgggcgca ttttccagat agcgatgcag
    16441 ataatcccga tacataggat gtgcacaatt atcaatgata gtgcgggcgc gttgaagcgg
    16501 ggaaagaccg cgcagatcgg cgatcccttg ttcggtgatg atcactttga cgctgtgttc
    16561 gttgtgatca acatggctgc acattggcac gacggttgag atcttgcctt ctttagcaat
    16621 cgacggagcc ataaagatcg acagatacgc gttgcgttca aaatcaccgc tgccgccgat
    16681 gccgttcatc agatcgaccc cggctacgtg cgttgagttg gcatgcccgt aaatatcaaa
    16741 ctccaggccg acgttcagag cgatgacgcc cagacgacgg atgatttccg ggttattgga
    16801 aatctcctgc ggacgcaaca caatgcggct ggcaaagtaa tccatattgt cgtaaatctt
    16861 gcgcagggaa tcggccgaga ttgtcaggct ggaggcgctg gccccgctga ttttgccggt
    16921 ttccagtaaa tgcaccaccg attcctgtag cacttccgaa tacatcataa acggaggaat
    16981 ttccgggttt tcccccagac gcgccattac cgcattattg atattgccca cgccactttg
    17041 cagcggcaga aattccggcg gaatacgccc atgcgccatt tcctgcaata agaacgtgac
    17101 cacgttatcg gcaatctgct ggcacatggg attttgctta tccagcatat taccggcgtc
    17161 gggcaagttg gtttccacga cggcgacaat ctttttcgga tcgatttgca catagcgggt
    17221 accgacgcga tccattgcat ggaagatcga cacgctattg cgccgcggtg gcgcgccagg
    17281 aatcacaata tccgccagtt ctgcaacgcg cggatcgtga tagtgattga gttcaatgat
    17341 cactttcttc gctcgcagca gccaggtcgg cgcattaccg atcccgctgg ttaaccagac
    17401 tcgaccatct ggtgccagtg ccgatgcttc aatgacggca acatcaatgt cgccgaagaa
    17461 accgtaattg accatttgcg ccacttcgct caaatgcagg tcaacgaaac tcaccgcgcc
    17521 ctgattgatc tttttacgta aaccggacga tgtttgatat ggcgcacgcc aggaaacagc
    17581 atcggcgtca gaaagtacat cgtcagcggc ggcgctgatt gacgcccccg tcagcaggcg
    17641 aatttgatac ggctttttgg cctcatgctg ttcgttagct ctgcgggcaa tcgcggtggg
    17701 tagggctttc ggcgaacccg ctggggtaaa gccgctaaat gccaccatgt cgttatgctg
    17761 gataatttct gccgcttcat cggcggtcat ccttgtccac tgagtttcca tttttactcc
    17821 ttcgttcgca gggattaatg accaacgaaa ttaggtttac gtttttcgag gaaagcgttc
    17881 atcccttcct ggtaatcttc gctgtcataa accgcgcggc gcatcccctg aatacgttca
    17941 aattcatcgg agttcatggt gtgtgcttcg cccagtacac gcagctcttc tttgataacg
    18001 gcaatggcta acggcgcttt ctcagagatg tggtgcgcca tttgtaaggt gaaatcttcc
    18061 agttcttcca cttccacaac atggttgagg atgccgacag ccagcgcgcg ctgggcggtg
    18121 atgggcgaag cggtaaaaat cagctctttg acaatgtgga agcccgcgtc gcgggtcagg
    18181 ttgtgaatgc cgaccaggtt atacgggacg ccgaggttta caggcgtcat tgagaaggtt
    18241 gaggtactgg cggcgataat cagatcggaa ctcatgatca tttcaaatgc gccaccccaa
    18301 acactacctt ccaccatcga aataatcggt ttcgggaatt tttggatcat gcgggtgatt
    18361 tgacgcaatg gatcatcata ggagagcgga tcgcgaccgc cagacggcag ttcgtgaata
    18421 tcgtgacctg cggagaagac tttggatcca ctcggcgcgc gcaaaatgat acagcgaatt
    18481 tccggccggt tgagatcgct taacgcctgc ataagatcat caataaagac tttacttaag
    18541 gcattaagtt ttcggccata gttaaactca atgaccgcca ctttgttgat agtgacaacg
    18601 ttaacatact gataagacat aaaaattcct ttaatcaaaa tattgcgtct ggataaattc
    18661 actgagctgc cgcaggccgg tgcgcggtga gagcgtattg tttttgaccg ctaaaagcgt
    18721 ctggcggtaa tagcgatcga aatcttcatt cgcgaacagg tgattcagta cttcttcttc
    18781 ggtctgctta cgcagccatt ccaccgattg ttgttgccgc acttgttgta aacgaccact
    18841 ggcagttagc gcggttttga agtcgatgat ggcgtgccag atctcatcga ttccacgttt
    18901 ttccagtgcg ctacaagtca gaacccgtgg ctgccattcg tcgtatttac gtcgcagaat
    18961 atgcagggca ctctcgtaca tatgccgggc aatggcgaca ttggtatggt tatcgccatc
    19021 gtctttgttg ataacgatca gatcagccac ttccatcagc ccttttttaa tgccctgcag
    19081 atcatcgccg ccaccggcaa tttgcaacga gataaaacag tccaccatgc gggcgacttc
    19141 tgtttccgac tgcccgacgc caaccgtttc gacaatcact acgtcataac ccgctgcttc
    19201 gcataacagc attaattccc gcgctcgctg actggcaccg cccagatgac cggaggatgg
    19261 taccgggcga ataaacgccg cttcggcacg cgccaggtca ttcatgcggg ttttatcccc
    19321 gagaatgcta ccgccagtga ccgggctgct gggatcgacc gcaataaccg cgacctttaa
    19381 tccctctcga atcaacaaca tgccaaaggc ctcaagaaag gtacttttcc ccgcgccggg
    19441 ggtgccggta acgcccagtc gcagggtgtt accgcagtac ggcataatgg catcaagcag
    19501 ctgcgtactt agtgcctgat gacgcgggtg acggctttcc accagcgtca tggcctgggc
    19561 gagtgtggca tgctcaccct gacgtaagcg gcgaatactt tctgccagcg tggcttcatt
    19621 aatcatgatg ctggcttatc agattcagta cgtcgcgcac actgtcgagc ataggtgtac
    19681 ctggaccata aatcgccgcc acgccgcgct cttgcaggaa ggcgtaatcc tgcggtggaa
    19741 tgacgccacc ggcgactacg cagatatctt cgcgtcccca ttttttcagc gcttcgacca
    19801 gttccgggat cagcgtttta tgaccggcag ccagtgagga tgcgcccact acgtgaacgt
    19861 cattttctac tgccaggcgg gcgatctctt caggtgtaga gaacatcggg cttaaatcta
    19921 cgtcgaaacc gagatcggaa taggcgctgg cgatcacttt cgcgccgcga tcgtgtccat
    19981 ccaggcccat tttggcgatc agaatgcgcg ggcgacgacc attgtcggca aggaactgct
    20041 ccgtttgcgc aacaatggca tcgaactcgg aggccgattt ctcagattga tgatagcttt
    20101 gcgcaatcac gccggtaaca cactggcttg gcaccagata acggtcgaaa gcggcttcca
    20161 gcgcatcgga aatttcaccc agggtggcgc gaacgcgagc ggcattaaca gcggcagcca
    20221 gcaggttttc gttatgctgt gcggcgtgag tcagggcgtt caacgcggcg gttacggcgg
    20281 catcatcacg ggtggcgcga atgcgttcca gcgaagcaat ttgctcgtta cgcaccatca
    20341 cgttgtcgat ctcaagtaca tcggtttcgt cttcgtgatc cagtttgtac ttgttgacac
    20401 caacgatgac acgcttgccc tggtcgatca gcgactgttc gcgcgctgag gcctcttcga
    20461 tcattcgttt tggcagacct gcttcgatcg ctttcgccat gccaccggct tcgtcgatct
    20521 gttggataat agctctggct tgtttgacga tttgatcggt cagcgactca atgtaatagg
    20581 atccggccag tggatcgacg gtgcggcaga gttctgattc ttcctggatg atgatctggg
    20641 tgttgcgggc aatgcgtgct gagaaatcgg taggcaaacc aagcgcttcg tcaaaggcgt
    20701 tggtatgcag tgactgagta ccgcccagcg tcgcagccag cgcttcaatg gtggtgcgga
    20761 taacgttgtt atacggatcc tgttcagtca ggctccagcc tgaggtctgg cagtgggtac
    20821 gcagcgccag tgatttcggg tcctgtgcgc caaatccact gaccgcttcg ctccataaat
    20881 aacgtgccgc acgcaacatg gcgacgttca taaacagatc catgccgatg ccgaagaaga
    20941 acgacaggcg aggagcgaag tcatcaattt tcagtccggc agagattgct gctttgatgt
    21001 actcaatccc atcagcgagc gtaaatgcta cctgctgcac gcagttggca cccgcttcac
    21061 ccatgtggta accgctgata ctgatggtat taaatcgcgg catgttgccg gaacaccagg
    21121 cgatgatgtc ggcgataatg cgcattgacg gttttggcgg gtaaatatag gtgttgcggc
    21181 agaggtactc tttgagaata tcgttttgaa tggtgccggt cagtttatca ggtgtaacac
    21241 cttgctcttc cgcggcgatg atataaaacg ccagtactgg tagcactgcg ccattcatgg
    21301 tcatcgaaac cgacatttta tccagcggga tctggtcgaa caggactttc atatcttcca
    21361 cggtgtcgat agcgacgccc gttttgccga cgtcacccgc cacgcgtggg ttatcggagt
    21421 cgtagccacg gtgggtggca aggtcaaacg caacggaaag acctttttgc ccggcggcca
    21481 ggttacggcg ataaaaagcg ttggactctt ttgctgttga aaaaccagca tactgacgga
    21541 tggtccacgg ttgggcggta tacatagtgg cacgcgggcc acgaacgtag ggcggcaaac
    21601 caggaagggt acctgtcacc tccagattat cgagatcggc ttcggtatac agcggcttga
    21661 tggcgatccc ttccgcggtt tgctgaacca gcgagtcgac agttttctcc cgacggctca
    21721 attccttgtt ggcaagctgt tgccactcct gctcgttaga cattatttgc tccgtaaaac
    21781 gcaatcaaaa tgagggtatt tgcgcttacg gtgagctttt tacgtgggtt tgtcgccagc
    21841 aaaaaaaatg cattcagacg tcgttttttg ccgcctgaga tttgtctatt ttgtgatgtg
    21901 attcaaggaa tacaaaaaga agtgaggata atgcctgata tgtgctatca ggcattattt
    21961 gatggattaa tcctgacgaa ggactttgtg accataatcg agcaacgcat cagtgacttt
    22021 acgcatcatg cggctttcag gagcaaagcg gtgccagtag agcatccggc gttgaaatag
    22081 ccctggcgtt aagtcaatca gttcaccgct ggctagttct ttctcgattt gcaggtgcgg
    22141 gatcatacag caggtggtgc cctggcgagc aagttgcacg aacgcttctg aagaattaac
    22201 gatatggcag ggcacgctgc ctggaggcag atcgaagttt tgctgcaaaa aggcctggtg
    22261 catatcgtca agatggtcaa acgcgaccac tggcgctttc agtaatgccg aacgcgttac
    22321 gccgttaggg aaatattttt cggcaaaggg ttttgagctg acgaacagat agtcgagcgc
    22381 accaagttta tcgacaagac aactcggcag cgcctgatgt tgaatactca ccgcgccgac
    22441 cacttcgccg cgacgcagac gttcctgagt gcgggtttca tcttctacct gcaagttgag
    22501 gcggataggc gaatcagcca atacaggagc cagtgccgga agcaaccacg tcgccagact
    22561 gtcggcattg accgccagtg aaagcagcag cggagtcgaa ccggtttgtt catcgcccag
    22621 ccactcttct tccagcaact ccacctggcg cagcagtgcc agcagttttt gcccttgttc
    22681 cgtcgggcgc ggcggtacgg tacgcaccaa cagcggctgc ccgaacatat tttccagttg
    22741 cttaatgcgc tgtgagacgg ctgattgtgt aatgcacagc ttttgtgccg cgcgctcaaa
    22801 tcctcgttca cgtatcaccg catccagtgc ctgtaatgtt ctgtagtccg ggcgtttcat
    22861 tgctgttgtc tgctcctggt aaatgctttt tcctgcacta tgacacaatt ttgtgtcagg
    22921 ttgcatatac cgcagccgtc ggcgggtaac atttgtatca atggttaaac gcgcagctta
    22981 aaagatcagg gatttgcgct attgcatttc gcttagcaac ttcgcgccct ggttatgttg
    23041 aatggcgtgg cgttattgcc tctgttcgca ccgtggagcg gcatacccag accgttgtat
    23101 gctttatttg cggggcgaaa ggggatgccc gccattgcgc gatattttat ggatgagtta
    23161 accacgcggc ttgccaacgg ggtctgaatc gctttttttg tatataatgc gtgtgaaatt
    23221 tcataccaca ggcgaaacga tcatgacgca ggatgaattg aaaaaagcag taggatgggc
    23281 ggcacttcag tatgttcagc ccggcaccat tgttggtgta ggtacaggtt ccaccgccgc
    23341 acactttatt gacgcgctcg gtacaatgaa aggccagatt gaaggggccg tttccagttc
    23401 agatgcttcc actgaaaaac tgaaaagcct cggcattcac gtttttgatc tcaacgaagt
    23461 cgacagcctt ggcatctacg ttgatggcgc agatgaaatc aacggccaca tgcaaatgat
    23521 caaaggcggc ggcgcggcgc tgacccgtga aaaaatcatt gcttcggttg cagaaaaatt
    23581 tatctgtatt gcagacgctt ccaagcaggt tgatattctg ggtaaattcc cgctgccagt
    23641 agaagttatc ccgatggcac gtagtgcagt ggcgcgccag ctggtgaaac tgggcggtcg
    23701 tccggaatac cgtcagggcg tagtgaccga taacggcaac gtgatcctcg acgtccacgg
    23761 catggaaatc cttgacccga tagcgatgga aaacgccata aatgcgattc ctggcgtggt
    23821 gactgttggc ttgtttgcta accgtggcgc ggacgttgcg ctgattggca cacctgacgg
    23881 tgtcaaaacc attgtgaaat gatctgacgg gggaacctcc cccgttaaaa aaattctctt
    23941 cattaaattt ggtgacatgt gtcacgtttt tacctggata ttgtcgattg ctctaaataa
    24001 atcctctaaa ccagcatatt caaccaagaa atacctttgc gtgatatttc ctcaacatcg
    24061 cgacgcaaac gttcatattg ccgcaatatt attttttgat atgttgaaag gcggatgcaa
    24121 atccgcacac aacatttcaa agacaggatt gggtaaatgg caaaggtatc gctggagaaa
    24181 gacaagatta agtttctgct ggtagaaggc gtgcaccaaa aggcgctgga aagccttcgt
    24241 gcagctggtt acaccaacat cgaatttcac aaaggcgcgc tggatgatga acaattaaaa
    24301 gaatccatcc gcgatgccca cttcatcggc ctgcgatccc gtacccatct gactgaagac
    24361 gtgatcaacg ccgcagaaaa gctggtcgct attggctgtt tctgtatagg aacaaaccag
    24421 gttgatctgg atgcggcggc aaagcgcggg atcccggtat ttaacgcacc gttctcaaat
    24481 acgcgctctg ttgcggagct ggtgattggc gaactgctgc tgctattgcg cggcgtgccg
    24541 gaagccaatg ctaaagcgca ccgtggcgtg tggaacaaac tggcggcggg ttcttttgaa
    24601 gcgcgcggca aaaagctggg tatcatcggc tacggtcata ttggtacgca attgggcatt
    24661 ctggctgaat cgctgggaat gtatgtttac ttttatgata ttgaaaacaa actgccgctg
    24721 ggcaacgcca ctcaggtaca gcatctttct gacctgctga atatgagcga tgtggtgagt
    24781 ctgcatgtac cagagaatcc gtccaccaaa aatatgatgg gcgcgaaaga gatttcgcta
    24841 atgaagcccg gctcgctgct gattaatgcc tcgcgcggta ctgtggtgga tattccggcg
    24901 ctgtgtgatg cgctggcgag caaacatctg gcgggggcgg caatcgacgt attcccgacg
    24961 gaaccggcga ccaatagcga tccatttacc tctccactgt gtgaattcga caacgtcctt
    25021 ctgacgccac acattggcgg ttcgactcag gaagcgcagg agaatatcgg cctggaagtt
    25081 gcgggtaaat tgatcaagta ttctgacaat ggctcaacgc tctctgcggt gaacttcccg
    25141 gaagtctcgc tgccactgca tggtggtcgt cgtctgatgc acatccacga aaaccgtccg
    25201 ggcgtgctaa ctgcgctgaa caaaatcttc gccgagcagg gcgtcaacat cgccgcgcaa
    25261 tatctacaaa cttccgccca gatgggttat gtagttattg atattgaagc tgacgaagac
    25321 gttgccgaaa aagcgctgca ggcaatgaaa gctattccgg gtaccattcg cgcccgtctg
    25381 ctgtactaat tccccttctc tgaaaattaa cgggcaggtc actgacttgc ccgttttttt
    25441 atcccttctc cacaccgttg ccgcgttgcg cattctactt tgcgagtctg ctccagaaaa
    25501 taaatcaaag tcagatcaat gcgttgtgtt tatttaatgg ggatttacga tggcgggaca
    25561 gcatggggct gtaacggtga agccctcacc gaagcgaggg cttaaaggag aagggttatg
    25621 atgcgacttg tcatcatact gattgtactg ttactgataa gtttcggagc ttattaacag
    25681 tcagtctcag gggaggagtc atcctccctt acccttactc actaaattag gtcaaagaat
    25741 caacgatgtc aatcagggcg atgcggggtg catcgccctg aaccgaactt tttgatagcc
    25801 acttcatcgc ccacgtttac tgccgtaatt aatcccttat tcctgcaatg tatgtctgcc
    25861 ttacatgacg ttttacgagt gcgctcgaag aaaagattta aaggtagatc aatgcgttgt
    25921 ggttatttaa agagagttta cgatggcagg gcagcatggg gctgtaacgg taaagccctc
    25981 accgaagcga gggcttgaag gagaagggtt atgatgcgac ttgtcatcat actgattgta
    26041 ctgttactga taagtttgcc agcttattaa cagtcagtct caggggagga gtcatcctcc
    26101 cttaccctta ctcactaaat taggttaaag aatcaacgat gtcaatcagg gcgatgcagg
    26161 tgtatcgccc ttaccactcc cagactttcg acggtgtaac caccgcagga agagggatat
    26221 cccactcttc aacggggagt ttttccacca actgacaatc atgcgcataa cccaccggtt
    26281 gcgttttata gtgctgccag ttttgtaagg tccgatcata aaaaccaccg cccattccca
    26341 ggcgctgacc gtactcatca aaggcgacca gcggtgtgat cagcacgtct aatcgggaaa
    26401 gggggagcac gtcacgcaca tccagttttg gctcatggat tttcaacctg ttcatcacca
    26461 gttcgctttg cggatggtaa ttcaggaaca gcaaattacc ggcactaaag ggatgcaaaa
    26521 ctggaagata tacgcgctta ccggcgcgcc agagttgttc tataagcggc tgggtgtcga
    26581 gttcaccatc aaaagagagg aatacagcga ccgtatgcgc catcaccacc gggggataag
    26641 tcatcatccg ggtagcggct tgttgaccca tttcctgctg ttgttccggc gttaacgcac
    26701 gacgacgttg ccgaatcatt ttgcggattt cttgtcggga taatgtcagt gggagttctg
    26761 gtagttgcgt catggctggt gccagataag aagggaatct ccgagatgcc gccgcaggct
    26821 gtaacccttg aacccttggt tcaaggtgaa tgtgtcgtcg cagttttaag gcttctcgga
    26881 cggaccgagc atgctcacca accgcggagc gccacattct tgtggtatga aatatcggct
    26941 caggggactg gcccgcttgc gaacatctca gagaaatttt gtcttcacgg ttactctacc
    27001 acagtaaacc gaaaagtgtc attcaaagtt ttggttagtt ttttcggtga tgcgaccttg
    27061 ttcaagtaac gcttgttcta tggtctgctg cagcatccga atacgctgtt ccatacttgc
    27121 cgcgtagtca cgagtcttcg ctttttcttg cgctaactca tagctgatat tcaatgcggc
    27181 aatgaagacc aactgttcag tatttgtgac tctagtgcgt tctttcagat cttgcaaccg
    27241 ttggttcaga tcgtccgctg cctgattcaa cgcatccctt tggtcaggcg ggcagttcac
    27301 acgcagtgaa cggccaaaaa tttggatatc gacgggttgt gcagacatgc caccttcctg
    27361 ctgattgact gcgctgcctt cgtctttaga cccatggtct gcgaaggggc gacactatag
    27421 ctaccctgat gagaagagac aagccctttt ctggtccacc agggcccaaa gtggtagcat
    27481 atcatgaata ttcctccctt tgacgacgaa tgcttatgtc tatacagaac gaaatgcctg
    27541 gttacaacga aatgaaccag tatctgaacc aacaagggac gggtctgacc cctgctgaga
    27601 tgcatggttt aatcagtggg atgatatgtg gcggtaacga tgacagctca tggctaccgc
    27661 tacttcacga cctgacgaac gaaggcatgg ctttcggtca tgagctggca caggcattgc
    27721 gtaaaatgca ctctgccacc agcgatgccc tgcaggatga cggcttcctt tttcagcttt
    27781 atctgcctga tggcgatgat gtcagcgttt tcgatcgggc tgatgcattg gcaggttggg
    27841 tcaatcactt cctgcttggt cttggtgtta cgcaaccgaa gctggataaa gtgaccggcg
    27901 aaaccggtga agctatcgac gatctgcgta acattgcgca actgggttac gacgaagacg
    27961 aagatcagga agagcttgaa atgtcgcttg aagagatcat cgaatacgtt cgtgttgccg
    28021 cgctgttatg ccacgacacc tttactcatc cgcaaccgac cgcgccagaa gtacaaaaac
    28081 cgactctaca ctaaaaacaa aaaacgtaag gagagtgtta tgagtgagat atcccggcaa
    28141 gagtttcagc gtcgccgtca ggccctggtg gagcaaatgc aacccggcag cgccgcgctg
    28201 atttttgctg caccagaagt aacacgtagc gccgacagcg aataccccta tcgtcagaac
    28261 agtgacttct ggtacttcac cggctttaac gaaccggaag cggtgttggt gctgattaaa
    28321 agcgatgaca ctcataacca cagcgttctg tttaaccgcg ttcgcgacct gacggcggag
    28381 atctggtttg gccgtcgctt aggccaggat gccgcgccag agaaactggg cgttgaccgc
    28441 gcactggcat tcagcgaaat taatcagcaa ctttatcaac tacttaatgg tctggatgtg
    28501 gtttaccatg ctcagggcga atatgcatat gctgatgaaa tcgtgaacag tgcgctggaa
    28561 aaactgcgta aaggttcgcg gcaaaatctc accgcaccgg caacgatgat cgactggcgt
    28621 cctgttgttc atgaaatgcg cctgttcaaa tcgccagaag agattgccgt actccgccgc
    28681 gcgggagaaa tcaccgccat ggcacataca cgggcgatgg aaaaatgccg tccgggaatg
    28741 ttcgagtacc atctggaagg cgaaattcac cacgaattta accgccacgg tgcgcgctat
    28801 ccgtcctata acaccattgt aggcagcggt gaaaacggct gcattttaca ctacaccgaa
    28861 aacgagtgtg aaatgcgcga cggcgacctg gtgttgattg acgcaggctg cgaatacaaa
    28921 ggttacgcag gcgatatcac ccgtaccttc ccggtcaacg gcaaattcac ccaggcccag
    28981 cgcgaaatct acgacattgt gctggagtct cttgaaacca gcctgcgcct gtatcgtccg
    29041 ggaacctcca ttctggaagt caccggtgaa gtggtgcgca ttatggttag cggcctggta
    29101 aaactcggca tcctgaaagg tgatattgat gaactgatcg ctcagaacgc ccatcgtcct
    29161 ttctttatgc atggccttag ccactggtta ggactggatg tccatgacgt tggcgtttat
    29221 ggtcaggatc gctcgcgcat tctggaaccg gggatggtac tgaccgtaga gcctggtctg
    29281 tatatcgcgc cggatgcgga tgtgccagaa caatatcgcg gtatcggcat tcgtattgaa
    29341 gacgacattg tgattaccga aaccggcaac gaaaacctca ccgccagcgt ggtgaaaaag
    29401 ccggaagaaa tcgaagcgtt gatggctgct gcgagaaagc aatgagcgta atcatcgtcg
    29461 gtggcggcat ggcgggcgcg acgctggcgc tggctatttc ccggttaagt cacggggcgc
    29521 tgccggtaca tttgattgaa gcgactgcgc cagagtcaca tgctcatccg ggctttgatg
    29581 gacgagcgat agcgctggcg gcgggtacct gtcagcaact ggcgcgcatc ggcgtttggc
    29641 aatctctggc ggattgcgca actgccatca ccaccgtgca tgtcagcgat cgaggtcacg
    29701 ctggatttgt caccctcgcc gcagaagatt accaactggc ggcgctggga caggttgtcg
    29761 aattgcacaa tgtcgggcaa cggctgtttg cattgctgcg taaagcacct ggcgtaacgc
    29821 tgcattgccc tgatcgcgtg gctaacgttg cccgtactca gagtcacgtt gaagtgacgc
    29881 tggagggtgg cgagacgctg acgggccgcg tgctggtagc cgctgatggc acccattcag
    29941 cgttagccac tgtgtgcggc gttgactggc agcaggagcc ttacgaacaa ctggctgtga
    30001 ttgccaacgt tgcgacttcc gttgcgcatg aagggcgcgc ttttgaacgc tttacgcaac
    30061 atggcccgct ggcgatgttg ccgatgtctg acggacgctg ttcgctggtc tggtgtcatc
    30121 cactggaacg gcgcgaagag gtgctgtcgt ggagcgacga gaagttttgc cgtgaactcc
    30181 agtcggcctt tggctggcga cttgggaaaa ttacccacgc tggtaaacgc agtgcttatc
    30241 cgctggcgtt aacccacgcc gccagatcta ttacccatcg taccgtgctg gtgggcaatg
    30301 cggcgcaaac cctgcacccg attgccgggc aagggttcaa cctcggtatg cgagatgtca
    30361 tgagtcttgc ggaaaccctg actcaggcgc aggagcgcgg agaagacatg ggtgattacg
    30421 gcgtattgtg tcgttatcag cagcgtcgac agagcgatcg cgaagcaacc attggcgtca
    30481 cggacagcct tgtacatctt tttgccaacc gttggacgcc actggttgtc gggcgcaaca
    30541 tcgggctgat gacgatggaa ttattcaccc cggcacgcga tgtgctggcg cagcgcaccc
    30601 tcggttgggt ggcgcgttga aggcggttat taaggaataa acaatgcaaa gtgttgatgt
    30661 agccattgtt ggcggcggca tggtggggct ggcggttgcc tgcggcttac agggtagcgg
    30721 cttacgcgtt gccgtactgg agcagcgcgt accggaacct ctggcggcgg atgcaccacc
    30781 acaactgcgc gtttcggcta tcaatgccgc cagcgaaaaa ttactcaccc gtcttggcgt
    30841 ctggcaggac attctctctc gccgagccag ctgttatcac ggtatggaag tgtgggacaa
    30901 agacagtttt ggtcacatct cgtttgacga tcaaagcatg ggctatagcc atcttgggca
    30961 catcgttgaa aactcagtga ttcactacgc gctgtggaac aaagcgcagc agtcgtcaga
    31021 tatcactctt ttggcccccg cagaattaca gcaggtcgcc tggggagaaa atgaaacctt
    31081 cctgacgctg aaagatggca gtatgttaac ggcgcgtctg gtgattggcg cggacggcgc
    31141 taattcctgg ttgcgcaaca aagccgatat tccgctgact ttctgggatt atcagcatca
    31201 cgcgctggta gcgaccatac gcacggaaga accgcatgat gcggtggcgc ggcaggtttt
    31261 ccatggcgaa ggcattctgg cctttttacc gcttagcgat ccgcatcttt gctcgattgt
    31321 ctggtcactg tcgccagagg aagcgcagcg gatgcagcag gcaagtgaag acgaatttaa
    31381 tcgcgcgtta aatatcgctt ttgataatcg cctgggctta tgcaaggttg agagcgcgcg
    31441 tcaggtgttc ccactgacgg ggcgttatgc gcgccagttt gccgcgcacc gtctggcgct
    31501 ggtgggcgac gccgcgcata ccattcaccc gctggcgggg cagggggtaa atcttggctt
    31561 tatggatgct gcagagctga ttgctgaact gaaacggttg catcgtcagg gtaaagacat
    31621 cgggcagtac atttatctgc gtcgctatga gcgtagccgc aagcacagtg cggcgctgat
    31681 gctggctggt atgcagggat tccgcgatct gttttctggt accaatccgg tgaaaaaact
    31741 gctgcgtgat attggtctga aactggccga cacgcttcct ggcgttaaac cacaacttat
    31801 ccgccaggca atgggattaa acgatttgcc tgaatggctg cgttaaaaat ttctcttctg
    31861 ctgtttattt gatacccatc acactttcat ctcccggttt tttcgccggg agattttcct
    31921 catttgaaat aaactaattt cacctccgtt ttcgcattat attttctaat gtcacgaatt
    31981 tttgatttag tgttttttga cattttttca gctcttaata ttgccttatt caaattaact
    32041 ttctcatcac atcatcttta tatagaaact tgtgtatttt ttggtttttt attctgttgc
    32101 gattttggcg ttttttaacc ataagctaat gtgatgatca attttacctt atggttaaca
    32161 gtctgtttcg gtggtaagtt caggcaaaag agaacgattg cgttggggac cgggagtggc
    32221 tccgatgctg ggtttcgtgg tgataatttc accatgaaaa agttgtcagc cccgcttatt
    32281 caatgaggac aagatggcac aacagactcc tttgtacgaa caacacacgc tttgcggcgc
    32341 tcgcatggtg gatttccacg gctggatgat gccgctgcat tacggttcgc aaatcgacga
    32401 acatcatgag gtacgtaccg atgccggaat gtttgatgtg tcacatatga ccatcgtcga
    32461 tcttcgcggc agccgcaccc gggagtttct gcgttatctg ctggcgaacg atgtggcgaa
    32521 gctcaccaaa agcggcaaag ccctttactc ggggatgttg aatgcctctg gcggtgtgat
    32581 agatgacctc atcgtctact actttactga agatttcttc cgcctcgttg ttaactccgc
    32641 cacccgcgaa aaagacctct cctggattac ccaacacgct gaacctttcg gcatcgaaat
    32701 taccgttcgt gatgaccttt ccatgatcgc cgtacaaggg ccgaatgcgc aggcaaaagc
    32761 tgccacactg tttaatgatg ctcagcgtca ggcggtggaa gggatgaaac cgttctttgg
    32821 cgtgcaggcg ggcgatctgt ttattgccac cactggttat accggtgaag cgggctatga
    32881 aattgcgctg cccaatgaaa aagcggccga tttctggcgt gcgctggtgg aagggggcgt
    32941 taagccatgc ggtctgggcg cgcgtgacac gctgcgtctg gaagcgggca tgaatcttta
    33001 tggtcaggag atggacgaaa ccatctctcc gttagccgcc aacatgggct ggactatcgc
    33061 ctgggaaccg gcagatcgtg acttcatcgg tcgtgaagcc ctggaagtgc agcgtgagca
    33121 tggcacagaa aaactggttg gtctggtgat gaccgaaaaa ggcgtgctgc gtaatgaact
    33181 gccggtacgt tttaccgatg cgcagggcaa ccagcatgaa ggcattatca ccagcgggac
    33241 tttctccccg acgctgggtt acagcattgc gctggcacgc gtgccgaaag gtattggcga
    33301 aacggcgatt gtgcaaattc gcaaccgtga aatgccggtt aaagtgacaa aacctgtttt
    33361 tgtgcgtaac ggcaaagccg tcgcgtgatt tacttttttg gagattgatt gatgagcaac
    33421 gtaccagcag aactgaaata cagcaaagaa cacgaatggc tgcgtaaaga agccgacggc
    33481 acttacaccg ttggtattac cgaacatgct caggagctgt taggcgatat ggtgtttgtt
    33541 gacctgccgg aagtgggcgc aacggttagc gcgggcgatg actgcgcggt tgccgaatca
    33601 gtaaaagcgg cgtcagacat ttatgcgcca gtaagcggtg aaatcgtggc ggtaaacgac
    33661 gcactgagcg actccccgga actggtgaac agcgaaccgt atgcaggtgg ctggatcttt
    33721 aaaatcaaag ccagcgatga aagcgaactg gaatcactgc tggatgcgac cgcatacgaa
    33781 gcattgttag aagacgagta acggctttat tcctcttctg cgggagagga tcagggtgag
    33841 gaaatttaca cctcaccctc actctcttcg caaggagaga ggttcacaat tcactgcacg
    33901 tttcaggaac catcgctcat gacacagacg ttaagccagc ttgaaaatag cggcgctttt
    33961 attgaacgcc atatcggacc ggacgccgcg caacagcaag aaatgctgaa tgccgttggc
    34021 gcacaatcgt taaacgcgct gaccggccag attgtaccga aagatattca gcttgcgacc
    34081 ccgccgcagg ttggcgcacc ggcgaccgaa tacgccgcgc tggcggaact caaggctatt
    34141 gccagtcgca ataaacgctt cacgtcttac atcggcatgg gttacaccgc cgtgcagtta
    34201 ccgccggtta tcctgcgtaa catgctggaa aatccgggct ggtataccgc gtatactccg
    34261 tatcaacctg aagtctcgca gggccgcctt gaggcactgc tcaatttcca gcaggtaacg
    34321 ctggatttaa ccgggctgga tatggcctct gcttctcttc ttgacgaagc caccgctgcc
    34381 gccgaagcaa tggcgatggc gaaacgcgtc agcaaactga aaaatgccaa ccgcttcttc
    34441 gtggcttccg atgtgcatcc gcaaacgctg gatgtggtcc gtactcgtgc cgaaaccttt
    34501 ggttttgaag tgattgtcga tgatgcgcaa aaagtgctcg accatcagga catcttcggc
    34561 gtgctgttac agcaggttgg cactaccggt gaaattcacg actacactgc gcttatcagc
    34621 gaactgaaat cacgcaaaat tgtggtcagc gttgccgctg atattatggc gctggtgctg
    34681 ttaactgcgc cgggtaaaca gggcgcggat attgtttttg gttcggcgca acgctttggc
    34741 gtaccgatgg gttacggtgg cccacacgcg gcattctttg cggcgaaaga cgaatacaaa
    34801 cgctcaatgc caggccgtat tatcggtgta tcgaaagatg cagctggcaa taccgcgctg
    34861 cgcatggcga tgcagactcg cgagcaacat attcgtcgtg agaaagcgaa ctccaacatt
    34921 tgtacttctc aggtgctgct ggcaaacatc gccagcctgt atgccgttta tcacggcccg
    34981 attggcctga aacgtatcgc taaccgcatt caccgtctga ccgatatcct ggcggcaggc
    35041 ctgcaacaaa aaggtctgaa gctgcgccat gcgcactatt tcgacactct gtgtgtggaa
    35101 gtggccgaca aggcgggcgt actggcgcgt gccgaagcgg ctgaaatcaa cctgcgtagc
    35161 gatatcctga acgcggttgg gatcaccctt gatgaaacca ccacgcgcga aaacgtgatg
    35221 cagcttttca gcgtactact gggggataac cacgggctgg acatcgacac gctggacaaa
    35281 gacgtggctc acgacagccg ctctatccag cctgcgatgc tgcgcgacga cgaaatcctc
    35341 acccatccgg tgtttaatcg ctaccacagc gaaaccgaaa tgatgcgcta tatgcactcg
    35401 ctggagcgta aagatctggc gctgaatcag gcgatgatcc cgctgggttc ctgcaccatg
    35461 aaactgaacg ccgccgccga gatgatccca atcacctggc cggaatttgc cgaactgcac
    35521 ccgttctgcc cgccggagca ggccgaaggt tatcagcaga tgatcgcgca gctggctgac
    35581 tggctggtga aactgaccgg ttacgacgcc gtttgtatgc agccgaactc tggcgcacag
    35641 ggcgaatacg cgggcctgct ggcgattcgt cattatcatg aaagccgcaa cgaagggcat
    35701 cgcgatatct gcctgatccc ggcttctgcg cacggcacta accccgcttc tgcacatatg
    35761 gcaggaatgc aggtggtggt tgtggcgtgt gataaaaacg gaaatatcga tctgactgat
    35821 ctgcgcgcga aagccgaaca ggcaggtgat aatctctcct gcatcatggt gacctatcct
    35881 tccacccacg gtgtgtatga agaaacgatc cgtgaagtgt gtgaagtcgt gcatcagttc
    35941 ggcggtcagg tttaccttga tggcgcgaac atgaacgccc aggttggcat cacctcgccg
    36001 ggctttattg gcgcagacgt ttcacacctt aacctgcata aaactttctg cattccgcac
    36061 ggcggtggtg gtccgggtat gggaccgatc ggcgtgaaag cgcatctggc tccgtttgta
    36121 ccgggtcata gcgtggtgca aatcgaaggc atgttaaccc gtcagggcgc ggtttctgcg
    36181 gcaccgttcg gtagcgcctc tatcctgcca atcagctgga tgtacatccg catgatgggc
    36241 gcagaagggc tgaaaaaagc aagccaggtg gcaatcctca acgccaacta tattgccagc
    36301 cgcctgcagg atgccttccc ggtgctgtat accggtcgtg acggtcgcgt ggcgcacgaa
    36361 tgtattctcg atattcgtcc gttgaaagaa gaaaccggca tcagcgagct ggatattgcc
    36421 aagcgcctga tcgactacgg tttccacgcg ccgacgatgt cgttcccggt ggcaggtacg
    36481 ctgatggttg aaccgactga atctgaaagc aaagtggaac tggatcgctt tatcgacgcg
    36541 atgctggcta tccgcgcaga aattgaccag gtgaaagccg gtgtctggcc gctggaagat
    36601 aacccgctgg tgaacgcgcc gcacattcag agtgaactgg tcgccgagtg ggcgcatccg
    36661 tacagccgtg aagttgcggt attcccggca ggcgtggcag acaaatactg gccgacagtg
    36721 aaacgtttgg atgatgttta cggcgaccgt aacctgttct gctcctgcgt accgattagc
    36781 gaataccagt aattcactga ttcgactatc ttctaaaggc gcttcggcgc ctttttagat
    36841 tgttaataaa gtgcgctttg tgtgtttcgg ctgaggcgta atataaaata ttgcgagctc
    36901 aatatattaa aagagtgcca ggcctgatga gcgtagcgca tcaggcacat cggctaatta
    36961 cagattctcg ccgttgctgg caatcacttc tttgtaccag ttaaagctct tcttacgtga
    37021 acgcgacata tcgccagtac cgtcgtcatg tttattcaca tagataaagc cgtagcgttt
    37081 gctgtactgc ccggtagtga acgacacgca gtcgatgcag ccccacggtg tgtagcccat
    37141 cagatccacg ccatcgtaag ttactgcttt tttcatctct tcaatatggg cgcgcaggta
    37201 gtcaatgcgg tagtcgtcgt tgatgctgcc atcttcttcc actttgtcgt aagcgccaaa
    37261 accgttttcg acaataaaca gcggcttctg gtaacgctca tacagttcgc aaagtgcgta
    37321 acgcagaccc accggatcaa tctgccagcc ccagtcagat gctttaacat acgggtttgg
    37381 tacgctgcct tcaaaaccag agatcgcatc gccggtgccg ccttcggcct tcactgcgtt
    37441 ggtcatgtaa tagctgaaac caagataatc gcaggtgcct tcgcgcagta catcgagatc
    37501 gctatcttcc attttgatgt taaatccgcg acgctcccac tcgttcaaca catacgacgg
    37561 gtaatagccg cgtagctgca catcggtaaa gacgtagcgt tcgcgcatcg actcctgagc
    37621 gaacatcaca tcgtccgggt tacatgagta gggatagagc ggcaccatcg ccagcataca
    37681 gccgactttc atctccaggt taatgcgacg cgcggctttc actgccaggg cgctggcgac
    37741 aaactggtga tgcagcacct gatacatcgt ctcttccggg ttttcatgct cggtatacac
    37801 cacgccggag cagcagtaac cgaacagcgg tgcacgccag ttacgctggt tgttaatttc
    37861 gttgaaggtc atccagtatt tgactttgtg cttatagcgt tcaaatacca cttccgcgaa
    37921 acgtacaaag aaatcaacca ctttacggtt ggtccagcta ccgtattgct gcaccagatg
    37981 cagcggcatt tcaaagtggg agagggtgat caccggttcg atgttgtatt tcagcagttc
    38041 atcgaacaga gagtcgtaga acttcagccc ttcttcgttt ggctgagctt catcgccttt
    38101 tggaaaaatg cgcgtccagg caatggatgt acgaaaacat ttgaagccca tttcggcaaa
    38161 tagcttgatg tcttccttat agtgaccata aaaatcaacg gcttcatggt ttggatagta
    38221 tttgcctggc aagacttctt tggttatttc gcgcggcacg ccgtgtgcgc caccggtcag
    38281 aacgtcacaa atgctcggcc cttttccgcc tttgttccag ccgccttcga cctgatgagc
    38341 ggcaactgcg ccgccccata agaaatcttt cggtaaggtg agttttttca ctatcatagg
    38401 ctccagatga gtcaattctt acaggaaata gtaccatgca gccaaacgac atcacttttt
    38461 ttcaacgttt ccaggatgac attctggctg ggcgtaaaac catcaccatc cgcgacgagt
    38521 ctgaatcgca cttcaaaacg ggtgatgtgc ttcgtgtcgg acgttttgaa gatgacggtt
    38581 atttttgcac gattgaagtc accgcaacct caaccgtaac gctggatacg ctgacggaaa
    38641 aacatgcaga gcaggaaaat atgaccctga ctgaactgaa aaaggtcatt gccgacatct
    38701 atcccggcca gacacagttt tatgtgattg aatttaaatg cctttaaatt gaactcacgc
    38761 ctgttagttg aaatttcgca tttaatttca tgatttttag gagattagtc tcaaatcaga
    38821 atttatttta gctaacaggt gttcactgga actattctca gttacgctgg agaggtatac
    38881 atgtgcgtgt acgtttctcc ggagtaagtt atggttcaga agcccctcat taagcaggga
    38941 tattcgctgg cagaggaaat tgccaacagc gtcagtcacg gcattgggtt ggtgtttggt
    39001 atcgttgggc tggtgttgct actggttcag gcggtggatc tcaatgccag cgccacagcg
    39061 ataaccagct acagcctcta tggcggcagt atgatcctgc tgttcctcgc ttcgacgctc
    39121 tatcacgcca ttcctcatca acgggcaaaa atgtggctga agaaatttga ccattgcgct
    39181 atttacctgt tgattgccgg aacctacacg ccgtttttgc tggtggggct ggattctccg
    39241 ttagcgcgcg ggttgatgat tgttatctgg agcctggcat tgctggggat tctgttcaaa
    39301 ctgaccatcg cgcaccgatt caaaatttta tctctggtga cttatctggc gatgggctgg
    39361 ctgtcgctgg tggtaattta tgaaatggca gttaagctcg cggcgggcag cgttacctta
    39421 ctggcggtag gcggcgtggt ttattcgctc ggggtgattt tctacgtctg caaacgcatt
    39481 ccatacaacc atgccatctg gcacggcttc gtgctcggcg gtagtgtgtg ccactttctg
    39541 gcgatctatt tgtatattgg gcaggcgtaa tgggtatgcc tttgtaggcc tgataagacg
    39601 cgccagcgtc gcatcaggcg ataaatgcct gatgcgacac agagatttac tcttcgagcg
    39661 aatacggcag tggctcgata cacaatgtat tcgcatcgtc gcgcacgcgg aagatgctgt
    39721 ccggttccat atcgttattc atcactacct gcaccacgac ctgaccatct tccagtttta
    39781 ccgcagccag caccgtacca gtacgacgcc agttctcgcc cattttcagc tctaagtctt
    39841 caccagcttc cggcagtcgg ctggcactac ctgtcagcaa ccagagcgcg cgtttgttgg
    39901 caccacggaa ttttgctcgc gccaccatct cttgtccggt atagcagcct ttcttaaagc
    39961 tgataccgcc cagcgcctgg aggttggtcg cctgtgggat aaactgcccg ctgttcgcgg
    40021 catcaatcac cgggaaaccc gcttcaatgt ttaatgccag ccactgttgg ctattgttca
    40081 gttccgcttc accgcgcagt ttatcggtca gcatattggc agtagcttca tcggttacga
    40141 tcaggaaacg ttctgccggg tgttcaaacc atagcaaagt ggtcgcgcct tctttaacta
    40201 cctgtttttc tttcgaaggc agttcactaa agatatttgc cagcgcggcg cgcgcctgaa
    40261 aaccggcaac accaagcagc acacgctcgt cgtctggcgc gatggtcact ttagagaaca
    40321 ccgcatattt tttcagttca gccagctgcg gttcacgcac gctgcgccgt tcaatccatg
    40381 caaagccatc gccgtcgcgg aacagacgta aattgctcca cattttacct ttggcgtcgc
    40441 aatgggcggc gagcaggtgc tgatcatctg tcatctggct gacatctgct gtcacctggc
    40501 cctgcaaata tttttcgctg tccgcgccag taatggtggc aagcgcccag tcatcaagcg
    40561 tcatcagcgt cagtggcaaa cgggcagaag ccgtaggctg acggggagga aaaggtgtaa
    40621 aagccataat agagtcctga gttgcttaac ggcgtattaa taactaatgg taaaagagct
    40681 atgaggcaat gcaagtgctt tcactacgcg atctgtgcct tctgcgaggc gacttcccaa
    40741 aaaatgaagc ccttaaattg ttgtgaatta atttcgcgat cgcgattgtg tttactgata
    40801 ttcccgtaaa agaagtcgaa aaacacgtta caatagccgg gtactggatt ttcgtgagac
    40861 aggaagaaga tggacattaa caacaaagcc cgcattcatt gggcatgccg ccgtggtatg
    40921 cgcgaactcg atatttcaat catgccgttt ttcgaacatg agtatgacag cttaagcgat
    40981 gacgaaaaac gcatctttat tcgtctgctg gaatgtgacg atcccgatct gtttaactgg
    41041 ctgatgaatc acggtaaacc ggccgatgca gaactggaaa tgatggtccg actcatccag
    41101 acacggaacc gggaacgtgg tcctgtggca atctgatttg cgcgtctcct ggcgcgcaca
    41161 gtggctttcc ttgctgattc atgggctggt tgccgctgtt attttactca tgccctggcc
    41221 gctcagttac accccgttat ggatggtgtt actttcgctg gtggtgtttg attgcgttcg
    41281 cagccagcgg cgtattaatg ctcgccaggg ggaaattcgc ttgttgatgg acgggcgttt
    41341 gcgttggcaa gggcaggagt ggagcatcgt caaagcgccg tggatgatta agagcggcat
    41401 gatgctgcgt ttacgttctg atagcggtaa acggcaacat ttatggctgg cagccgacag
    41461 catggacgaa gccgaatggc gggatttacg gcggattttg ttgcaacaag agacgcaaag
    41521 ataaagatga cggcaaagca tatgcgctaa gccgtcagtg atatcaggcg taatgctctg
    41581 ccatttcgtt gaggatttgc tcgcaccagc tctgaatacg ctcgtcgcta aggtcatact
    41641 ggttagtttc gtccagcgcc agccccacga acagttgccc gtcagcaatc accggtttcg
    41701 ggctggtaaa ttcataccct tccgttggcc agtagccgac gaacttcacg cctttggtcg
    41761 agagtttgtc atgcagcata ccgagcgcat cgaggaacca ctcgccgtat cccagttgat
    41821 cgccaagccc atacagcgca acaattttac cttcgaggtt caggtcgtcg agctgatccc
    41881 agacggcttc ccagtcttcc tggatttcgc caaaatccca ggtcgggata cccagaatga
    41941 gcacatcgta ctgctccatt aatttcgggg agtcgtcctt gaggttatgt aaggtcacca
    42001 gttctgggcc gataatatcg cggatttttt ctgccgccat ttcggtgtaa caggtgctgg
    42061 aaccgtaaaa aagacccata ttcataacgt aagcttctcg attctgctgt ggcttttgcg
    42121 gctagtgtat cagaatcgct ttagtcacgg cacaataagg cataatggat caaatgagtg
    42181 accataaggg gcgcaagtga aacaggatct ggcacgcatc gagcagtttc ttgatgctct
    42241 gtggctggaa aaaaatctgg ctgaaaatac gttgaacgct taccgtcgcg atctgtcaat
    42301 gatggtggag tggttgcatc accgcgggtt gacgctggcg acggcgcaaa gtgacgattt
    42361 acaggcatta ctggcagaac ggctggaggg cgggtataaa gccaccagct cagcgcgttt
    42421 gctgagtgca gtgcgccgat tgttccagta tctttatcgc gaaaagtttc gtgaagatga
    42481 tcccagtgcg catctcgctt caccgaaatt gccccagcgt ttgccaaaag atttaagcga
    42541 agcgcaggtc gaacgtttat tacaggcacc attaattgat cagccactgg agctacgcga
    42601 taaagctatg cttgaagtgt tgtatgctac cgggctgcgt gtctctgaac tggtcgggct
    42661 gacaatgagt gatatcagcc tgcgtcaggg cgtagtgcgg gtcattggta aaggcaacaa
    42721 agagcgcctg gtgccgttag gtgaagaggc ggtttactgg ctggaaacct atctggaaca
    42781 tgggcgtccg tggctactga atggtgtgtc aattgacgtg ttgtttccca gccagcgtgc
    42841 gcagcagatg acgcgacaga ccttctggca ccgcattaaa cattatgccg tgctggcggg
    42901 tatcgacagc gaaaagctgt caccgcatgt gttgcgtcac gcttttgcca ctcacttatt
    42961 aaatcatggt gcggatttac gcgtggtgca gatgctactg ggccacagcg atctctccac
    43021 cacgcaaatt tatacgcatg tcgctaccga gcgtctgcgg caacttcatc aacagcatca
    43081 cccgcgggcg tgatgtctga aaagaacggg aagatttatg aagaaaggtt ttatgttgtt
    43141 tactttgtta gcggcgtttt caggcttcgc tcaggctgat gacgcggcaa ttcaacaaac
    43201 attagccaaa atgggcatca aaagcagcga tattcagccc gcgcccgtag ctggcatgaa
    43261 gactgtgctg actaacagcg gtgtgttgta catcaccgat gatggtaaac atatcattca
    43321 ggggccaatg tatgacgtta gcggcacggc tccggtcaat gtcaccaata agatgctgtt
    43381 aaagcagttg aatgcgcttg aaaaagagat gatcgtttat aaagcaccgc aggaaaaaca
    43441 cgtcatcacc gtgtttactg atattacctg tggttactgc cacaaactgc atgaacaaat
    43501 ggcagactat aacgcgctgg ggatcaccgt gcgttatctt gctttcccac gccaggggct
    43561 ggacagcgat gctgagaaag aaatgaaagc tatctggtgt gcgaaagata aaaacaaagc
    43621 gtttgatgat gtgatggcag gtaaaagcgt cgcaccagcc agctgcgacg tggatattgc
    43681 cgaccattac gcacttggcg tccagcttgg cgttagcggt actccggcag ttgtgctgag
    43741 caatggcaca cttgttccgg gttaccagcc gccgaaagag atgaaagaat tcctcgacga
    43801 acaccaaaaa atgaccagcg gtaaataatt cgcgtgaaac aacagataca acttcgtcgc
    43861 cgtgaagtcg atgaaacggc agacttgccc gcggaattgc ctcccttgct gcgccgttta
    43921 tacgccagcc ggggagtacg cagtgcgcaa gaactggaac gcagtgttaa aggtatgctg
    43981 ccctggcagc aactgagcgg cgtcgaaaag gccgttgaga tcctttacaa cgcttttcgc
    44041 gaaggaacgc ggattattgt ggtcggtgat ttcgacgccg acggtgccac cagcacggct
    44101 ctaagcgtgc tggcgatgcg ctcgcttggt tgcagcaata tcgactacct ggtaccaaac
    44161 cgtttcgaag acggttacgg cttaagcccg gaagtggtcg atcaggccca tgcccgtggc
    44221 gcgcagttaa ttgtcacggt ggataacggt atttcctccc atgcgggagt tgagcacgct
    44281 cgctcgttgg gcatcccggt tattgttacc gatcaccatt tgccgggcga cacattaccc
    44341 gcagcggaag cgatcattaa ccctaacttg cgcgactgta atttcccgtc gaaatcactg
    44401 gcaggcgtgg gtgtggcgtt ttatctgatg ctggcgctgc gcaccttttt gcgcgatcag
    44461 ggctggtttg atgagcgcgg catcgcaatt cctaacctgg cagaactgct ggatctggtc
    44521 gcgctgggga cagtggcgga cgtcgtgccg ctggacgcta ataatcgcat tctgacctgg
    44581 caggggatga gtcgcatccg tgccggaaag tgccgtccag ggattaaagc gctgctggaa
    44641 gtggcaaacc gtgatgcaca aaaactcgcc gccagcgatt taggttttgc gctggggcca
    44701 cgtctcaatg ctgccgggcg actggacgat atgtccgtcg gtgtggcgct gttgctgtgc
    44761 gacaacatcg gcgaagcgcg cgtgctggca aatgaactcg atgcgctaaa tcagacgcga
    44821 aaagagatcg aacaaggaat gcagattgaa gccttgaccc tgtgcgagaa actggagcga
    44881 agtcgcgaca cgctacccgg cgggctggca atgtatcacc ccgaatggca tcagggcgtt
    44941 gtcggtattc tggcttcgcg catcaaagag cgttttcacc gtccggttat cgcctttgcg
    45001 ccagcaggtg atggtacgct gaaaggttcc ggtcgctcca ttcaggggct gcatatgcgt
    45061 gatgcgctgg agcgattaga cacactctac cccggtatga tgctgaagtt tggcggtcat
    45121 gcgatggcgg cgggtttgtc gctggaagag gataaattcg aactctttca acaacggttt
    45181 ggcgagttgg ttaccgagtg gctggaccct tcgctattgc aaggcgaagt ggtgtcagac
    45241 ggcccgttaa gcccggccga aatgaccatg gaagtggcgc agctgctgcg cgatgctggc
    45301 ccgtgggggc agatgttccc ggagccgctg tttgatggtc atttccgtct gctgcaacag
    45361 cggctggtgg gcgaacgtca tttgaaagtc atggtcgaac cggtcggcgg cggtccgctg
    45421 ctggatggta ttgcttttaa tgtcgatacc gccctctggc cggataacgg cgtgcgcgaa
    45481 gtgcaactgg cttacaagct cgatatcaac gagtttcgcg gcaaccgcag cctgcaaatt
    45541 atcatcgaca atatctggcc aatttagcgt catcttctct ataaaaaaga gcgtggattg
    45601 ggtacaatcc cgctcttatc accgcatttt gactagctca ataaaagaaa tcagaccatg
    45661 tttgaaatta atccggtaaa taatcgcatt caggacctca cggaacgctc cgacgttctt
    45721 agggggtatc tttgactacg acgccaagaa agagcgtctg gaagaagtaa acgccgagct
    45781 ggaacagccg gatgtctgga acgaacccga acgcgcacag gcgctgggta aagagcgttc
    45841 ctccctcgaa gccgttgtcg acaccctcga ccaaatgaaa caggggctgg aagatgtttc
    45901 tggtctgctg gaactggctg tagaagctga cgacgaagaa acctttaacg aagccgttgc
    45961 tgaactcgac gccctggaag aaaaactggc gcagcttgag ttccgccgta tgttctctgg
    46021 cgaatatgac agcgccgact gctacctcga tattcaggcg gggtctggcg gtacggaagc
    46081 acaggactgg gcgagcatgc ttgagcgtat gtatttgcgc tgggcagaat cgcgtggttt
    46141 caaaactgaa atcatcgaag agtcggaagg tgaagtggcg ggtattaaat ccgtgacgat
    46201 caaaatctcc ggcgattacg cttacggctg gctgcgtaca gaaactggcg ttcaccgcct
    46261 ggtgcgtaag agcccgtttg actccggcgg tcgtcgccac acgtcgttca gctccgcgtt
    46321 tgtttatccg gaagttgatg atgatattga tatcgaaatc aacccggcgg atctgcgcat
    46381 tgacgtttat cgcgcgtccg gcgcgggcgg tcagcacgtt aaccgtaccg aatctgcggt
    46441 gcgtattacc cacatcccga ccgggatcgt gacccagtgc cagaacgacc gttcccagca
    46501 caagaacaaa gaccaggcca tgaagcagat gaaagcgaag ctttatgaac tggagatgca
    46561 gaagaaaaat gccgagaaac aggcgatgga agataacaaa tccgacatcg gctggggcag
    46621 ccagattcgt tcttatgtcc ttgatgactc ccgcattaaa gatctgcgca ccggggttga
    46681 aacccgcaat acgcaggccg tgctggacgg cagcctggat caatttatcg aagcaagttt
    46741 gaaagcaggg ttatgaggaa ccaacatgtc tgaacaacac gcacagggcg ctgacgcggt
    46801 agtcgatctt aacaatgaac tgaaaacgcg tcgtgagaag ctggcgaacc tgcgtgagca
    46861 gggaattgcc ttcccgaacg atttccgtcg cgatcatacc tctgaccaat tgcacgcaga
    46921 attcgacggt aaagagaacg aagaacttga agcgctgaac atcgaagtcg ccgttgctgg
    46981 ccgcatgatg acccgccgta ttatgggtaa agcctctttt gttaccctgc aggacgttgg
    47041 cggtcgtatt cagctgtacg ttgcccgtga cgatcttccg gaaggcgttt acaacgagca
    47101 gttcaaaaaa tgggacctcg gcgacatcct cggcgcgaaa ggtaaactgt tcaaaaccaa
    47161 aaccggcgaa ctgtctatcc actgtaccga gttgcgtctg ctgaccaaag cactgcgtcc
    47221 gctgccggat aaattccacg gcttgcagga tcaggaagcg cgctatcgtc agcgttatct
    47281 cgatctcatc tccaacgatg aatcccgcaa cacctttaaa gtgcgctcgc agatcctctc
    47341 tggtattcgc cagttcatgg tgaaccgcgg ctttatggaa gttgaaacgc cgatgatgca
    47401 ggtgatccct ggcggtgccg ctgcgcgtcc gtttatcacc caccataacg cgctggatct
    47461 cgacatgtac ctgcgtatcg cgccggaact gtacctcaag cgtctggtgg ttggtggctt
    47521 cgagcgtgta ttcgaaatca accgtaactt ccgtaacgaa ggtatttccg tacgtcataa
    47581 cccagagttc accatgatgg aactctatat ggcttacgca gattacaaag atctgatcga
    47641 gctgaccgaa tcgctgttcc gtactctggc acaggatatt ctcggtaaga cggaagtgac
    47701 ctacggcgac gtgacgctgg atttcggtaa accgttcgaa aaactgacca tgcgtgaagc
    47761 gattaagaaa tatcgcccgg aaaccgacat ggcggatctg gacaacttcg actctgcaaa
    47821 agcgattgtt gaatctatcg gtatccacgt tgagaagagc tggggtctgg gccgtatcgt
    47881 taccgagatc ttcgaagaag tggcagaagc acatctgatt cagccgacct tcattactga
    47941 atatccggca gaagtttctc cgctggcgcg tcgtaacgac gttaacccgg aaatcacaga
    48001 ccgctttgag ttcttcattg gtggtcgtga aatcggtaac ggctttagcg agctgaatga
    48061 tgcggaagat caggcgcaac gcttcctgga tcaggttgcc gcgaaagatg caggtgacga
    48121 cgaagcgatg ttctacgacg aagattacgt caccgcactg gaacatggct taccgccgac
    48181 agcaggtctg gggattggta tcgaccgtat ggtaatgctg ttcaccaaca gccataccat
    48241 ccgcgacgtt attctgttcc cggcgatgcg tccggtgaaa taagcattac gttatgcgca
    48301 taaccccggc agatgtcggg gttttttaat tgtgctgcgc gaaagcagac aacaattttc
    48361 ttgcttcact attggctgcc tgcatcacca tccacggact gaacgcccac ggcgtggcat
    48421 caataccgtg taatacatct gctaaatcac accattgata atccatcact tcatcatcgt
    48481 tgatctgtaa cgcactggtg gtgcgtgcgg caaataccgg acacacttca ttttccacaa
    48541 tgccattcgg atcggtggcg cgatagcgaa agtcaggata gatagattca ggaggcgtaa
    48601 tttccacgcc aagctcataa cggcaacggc ggatcaccgc gtcttcgttg ctttctccca
    48661 gttgtgggtg cccacaaacc gagttagtcc acacgccagg ccatgctttt ttgctcagtg
    48721 cgcggcgggt aactaataat tgtcctttgg cattaaacag ccaactggag aacgcgagat
    48781 gtaagcgggt gtctgccgtg tgtgcggcat acttttccag cgtacccgtg ggaactccct
    48841 gtgcattcaa taaaatgacg tgttccgttt gcataatttc tcacatgtaa ttctgatcac
    48901 tcatcattat agataattga agagtgaaat gattaaccaa gtgagcgttt atcgacaacc
    48961 gcccgttttg agcggatgcc gacaggtaaa aaccatttaa aaaagtagca tcatatattt
    49021 gaagcgactt tcaatggcca ttgcaacaca gtcagaaatg acaggaatag agagagtaaa
    49081 aagcaacagc agactgaata tagcgcgcat taaaaaattc atattattct ccatgctcat
    49141 ttttcttcag cttgcgcatc agaagccaca tacgtactgt cctgactttt aaatctttat
    49201 cagagacgga ttccttcacc aggtcagcat gatgctgata gccattaaag aagacgttca
    49261 gcacaacggc gctaagggtt gcgagcataa tgccgctatg tagcagcggt tgcagtacgg
    49321 ccggtaactt agaaaagaaa tcgtgagaga gcgttggagt catgccaacg ccgagactga
    49381 tcgccacaat atagaggttg taacggttgg tggtgtagtt acagcgcgac agaattcgaa
    49441 tccctgtcgc cagtaccatg ccgaacatca ccagaccagc gccgcccagc acaaattgcg
    49501 gaatggaggc taccagcacc gccatttttg gcaccatgcc aaacaggatt aatataattc
    49561 ccgaagaaat acacacccag cggctatgaa cgcgcgtcac gctaaccagg ccaacgtttt
    49621 gagaaaatga cgtgtggggg aagctgttaa acgtgccacc aatcattgtc cctacgccat
    49681 cgacacgcag cccgcgaata atatcgtgcg aagagagttt acgaccgact atttcaccca
    49741 gtgccaggaa catccccatc gactcgataa acacgatgat taacacggca gtcatggtca
    49801 gaatggaaac gggatcgaaa atcggcatac caaacgacat cggggtgaca atcgcaaacc
    49861 acgaggcatc atgtagcccg gataaattga cttcattcat catccacgaa agtaaaaagc
    49921 caaatacaat ccccagtaat acggcgacgt tggacataaa tcctttcgca tagcgagtaa
    49981 tgagcaagat aaaaattaag acggcaaagg agatacctaa ataaacggga ttaccatatt
    50041 gcggattccc tttacctccg gcggcccagt caatacccac ctgaatgatg ctaagcccga
    50101 tagaagtaat aaccacaccg gtaaccagtg gcgggaataa aggcatcaag cgaccgataa
    50161 gttgcgctaa taatgtggtg ataaaacccg cggcgatagt ggcaccaaat atacccagca
    50221 ggccgatatc cgggttcatc ccaatggcta tcattggtgt tacagcagca aaggtcaccg
    50281 acataatcac cggcaggcgg atccccataa agcggccgat accgatacat tgcaataatg
    50341 tgacgatccc gcagcaaaag agatccgagc taatgagcat cgcaatagct tcttttgaga
    50401 ggcccagtcg gtcaccaatc ataagaggaa cagcgactgc acctgcgtac atgacaagga
    50461 cgtgttgcag accgagtatg atcagctttc ctggtgataa tatgcggtca acctcatcag
    50521 ttgggcggtc ttgcccagaa gatgagggaa gttgggaatc tatggcgctc atcagtgttt
    50581 ctcctttcgc ccgccagagt aaaaagcaac cgtttacgtt aatgaaactg tctaaacaca
    50641 tgctcgtgga gtgccagttt ttgcgaggaa ggggagttag agacaggaat tgcggaggca
    50701 atcacataat cgtgcttaaa ctacgaaaat gacaagagaa cctgacaatg gaattatctg
    50761 taattttctc atattgatat ttcattaatc attctggcat gtcgcactct cgcatttaat
    50821 cgtttttatc tggatagcgc tatttggggc ggcaatgttg aaataatgaa agcggcatag
    50881 attgaggggc aataatttga actcagtcgc aaaattataa ttaaaacggt catgctacct
    50941 aatatatgcc cgtttattgc atcgcgaaat ttgagaagac ataactcatt aaatgaaagg
    51001 gatgcaaatg aataagttta tcgctgctga agccgcggaa tgtataggct gtcatgcttg
    51061 tgaaattgcc tgtgcggtgg cacacaatca agaaaactgg ccgctgagtc acagtgactt
    51121 tcgaccacgt atccacgttg tagggaaagg cctggctgcg aatccggtgg cctgccatca
    51181 ctgcaacaat gccccttgcg ttacggcttg tccggttaat gctctgactt tccagtccga
    51241 tagcgtacaa ctggacgagc aaaaatgtat tggttgtaaa agatgcgcaa tcgcttgccc
    51301 ctttggcgtc gttgagatgg tcgatacgat tgcgcagaaa tgcgaccttt gtaaccagcg
    51361 cagttccggc acgcaagcct gtattgaagt ttgcccaaca caggcgttac gactgatgga
    51421 cgataaaggg ttacagcaga taaaggtggc ccgccagcgc aaaacggcag caggaaaagc
    51481 gtcatcagac gctcagccat ctcgcagtgc agcgttgctc cccgttaact cgcgtaaagg
    51541 cgcagataaa atttcagcaa gtgaacggaa aaaccacttt ggcgaaatct attgcgggct
    51601 ggatccacaa caagcgactt atgagagtga ccgctgtgtt tattgtgccg aaaaagctaa
    51661 ctgcaactgg cattgcccgc tgcataacgc tattccggat tacatccgtc tggtacagga
    51721 gggaaagatt attgaagcgg cagaactttg ccaccagacc agttccttac cagaaatctg
    51781 cggcagggta tgtccacagg accgtctttg tgaaggcgca tgtactttga aagatcactc
    51841 tggcgcagtc tctatcggta atctggaacg ctacatcacc gataccgcgc tggagatggg
    51901 ctggcgtccc gatgtcagca aagttgttcc ccgtatcgag aaagtggcgg tgattggcgc
    51961 tgggcctgca gggttagggt gtgctgatat tctggcgcgc gcaggagttc aggtcgatgt
    52021 ctttgatcgc catccagaaa ttggcggtat gctgactttt ggcattcctc ctttcaaact
    52081 cgataaaacg gtattaagcc agcggcgaga gatattcacc gcaatgggaa tcgactttca
    52141 tcttaactgt gaaattggcc gcgatattac ctttagcgat ttaacttctg aatatgatgc
    52201 agttttcatc ggcgtgggga cttacgggat gatgcgagca gatctgccgc atgaagatgc
    52261 gcccggtgtc attcaggctc taccgttcct gactgcccat acccgccagc tcatgggatt
    52321 gccggagtct gaagagtatc cgctgacgga cgtggaaggt aagcgagtcg tggtattggg
    52381 cggtggcgat acgacaatgg attgtttgcg gacttccatt cgcctcaatg ctgccagcgt
    52441 gacctgcgcg tatcgtcgtg atgaagtcag tatgccgggc tcgcgcaaag aggtggtcaa
    52501 tgcgcgcgag gaaggtgtcg agtttcaatt caatgttcag ccgcaatata tcgcttgtga
    52561 cgaagatggg cgtttaactg cggtgggcct gattcgtacc gccatgggtg agccggggcc
    52621 ggatggtcgc cgtcgtcctc gtccggttgc gggttcagag tttgaattgc ccgccgatgt
    52681 tctcattatg gcctttggtt tccaggcgca tgccatgccg tggttgcagg gcagcggaat
    52741 taaactcgat aaatggggcc tgattcaaac cggtgacgtc gggtatttac ctacccagac
    52801 gcatctgaaa aaagtctttg ctggtggtga tgcagttcat ggcgcggatc tggttgtcac
    52861 tgcaatggcc gcaggaaggc aggcggcgcg cgatatgtta actctgtttg atacgaaggc
    52921 atcgtgatga aatcgttaat tatcgttaat ccggctgact gtattggctg ccgtacttgt
    52981 gaagtggcct gtgtggtcgc tcacccttca taacaggagt tgaatgccga tatcttttta
    53041 ccccggctga aggtacagcg actggactgc atcagtgcgc cggtgatgtg ccatcagtgt
    53101 gaaaacgccc cttgtgttgg cgcttgcccc gtgggggcgc tgacgatggg cgagcaggtg
    53161 gtgcagacca attctgcccg ttgtattggc tgtcagagtt gcgtcagtgc gtgcccgttt
    53221 ggaatgatca ccattcagtc gttgccgggg gatacccggc aacaaatcgt gaaatgcgat
    53281 ctctgtgaac agcgggaaga ggggcctgcc tgcgttgaat cttgcccaac acaggcgctg
    53341 cagttgctta ctgaaagaga actcaggcga gtccgccagc agcgtattgt tgccagcggt
    53401 gagaatcctc tgtgaaagaa atctccgcgc tgaaaggcgc ggagaatcta ttagattgcc
    53461 caaccacccg cgtaaaatgc gaccagtgcg gcagtaatga tcaccgtacc aatattcagc
    53521 ttttgccatt cgcgtgcaaa gacgcggcct acgaccagtg tcacaaagcc cagcataata
    53581 ccggtaacga tattacaagt cagaacgatg aacacggcac acaccaggcc agccatagcg
    53641 tcaataaaat cattgaaatc cagcttcgag acgttactta acatcagcaa acctacgtac
    53701 atcagagcgg gtgcagtggc gtaaccaggg atcaaaaatg acagcggtga taagaacaga
    53761 atcagcagga ataacgcccc cactacggtt gcggttaacc ctgttttacc cccggcggcg
    53821 gttcctgccg ctgattcgat ataaaccgct gcgggcgctg cgcccaccag gccggagaat
    53881 attgaactta ctgagtcact ggtcagggct ttgccgccgt tgatgatctg gttgtcttta
    53941 tccaacaaat tcgcctgacc ggcgacggca cggatggtgc cagtagcgtc gaacactgcg
    54001 gtcatcacca atgccagtac actcggaagt acagttggct ggagtgcgcc cataatatcg
    54061 aggctgaaaa tcagagactt accatcttcg ccagtcaggc ttggcatcgc caccagaccg
    54121 tggtatttca ccgctgggtc aaagattaag ccgatgatcg aaattgcaat aatcaccaac
    54181 aagataccgc cgggtacgcg acacttctcc agaccgaaga tgaccgccag ccccagcaag
    54241 ctcatcatca ccgggaagga ggtaaacgca ccgagcgcca ctggcaagcc ttcaatcggg
    54301 tttttgataa ccatacccac accgttagca gcaatcagca gcagaaacag cccgataccg
    54361 atacctgtac cgtgagcgat acccatcggc aaattacgta agatccaggt acgcacaccg
    54421 gttacggaaa tggcggtgaa gatgacgccc atcagaaata ccgcgcccag tgcgacagga
    54481 acgctaattt gttgcccgag taccagactg aatgcggtaa acgccgtcaa ggaaatcgcg
    54541 caaccaatcg ccattggcaa attagcccat aaccccatca gcaacgagcc gaagcccgcg
    54601 accagacagg tggcaacaaa cacagctgcg ggaggaaaac ctgctttgcc cagcattccc
    54661 ggaacgacga taacggaata aaccatggcc agaaaggtcg ttaagccagc cagtacttcc
    54721 tgacgaacgg tactgccacg agcggtaatt ttaaaataat tatccagcgc gccctgtggc
    54781 tttggtgcgt ccggtgtttg taggatgtct ccagacataa tgatgtcctc acgaggatgt
    54841 aaaagtttta ttattagttg cgttcgtaca ccagacgacc atcaacgtag gtgcggtaga
    54901 tcgaacggtc atcgcccaac gtcatcatca cgaacaattt gtcgactaaa gaaacagagt
    54961 tgtcatagcg cagctgctgt agcggagtgg cggtgggttc catcaccacg aaatcagcct
    55021 ctttgccagg taaaaagttg ccaatcaaat cgtcaaggcc cagagatttc gctccgccga
    55081 gcgtggccag gtaaaacgct tcatatgccg agaggcgata gccttgtaat tgcaacactt
    55141 tgtaggcttc gttcagcgtt tgcagcatgt tgaaagtggt tccggcaccg atatccgttc
    55201 ccatgcccac tttaactttc ttctgccatg cttttttcaa gttgaataac ccgctgccga
    55261 ggtaaaggtt ggaggtcgga cagaaagcaa tgctggattt ggtttcgctg agacgatccc
    55321 actctttttc tttgagatgg acgcagtgag caaagacaca gtttttaccg gtcaggccgt
    55381 actgatggta aacatccaga taaccatcat ggtcaggata aagcgatttc acccaggcaa
    55441 tttcatcttt gttttcacag agatgggtat gtacccacgt atccggatat tcctctttca
    55501 ggcgttgcgc catcgccatc tgttcaggag aagaagtagg ggcgaagcgt ggcgtaatcg
    55561 catatagcag acgaccattt ttgtgccagc gttcaatcag ttctttgctt tggtgatagc
    55621 tgctttcggc agtgtctagc agataatccg gtgcgttgcg gtccatcatc accttaccgg
    55681 caatcatacg catattgata tgactggcgg cttcaaacag cgcatcaacg gattgcggat
    55741 gaacagtgcc aaacaccagc gccgtggtgg ttccgttacg taaaagctgc ttgatgaaga
    55801 acgccgacat ttcgcgggcg tactctaaat cctcataacg acgttcagta gggaaggtgt
    55861 gtttattcaa ccactccagc aattgttccc cataggcccc caccatttca ctttgcggat
    55921 aatggatatg tgtatcgaca aagcccggta ctatcagttt gccgcgatag tcgcgcacgc
    55981 gaatagtgtc aggaatttga tgctttccgt cttcccattc gccaaaccat tccacttttc
    56041 cctgtttaat gagtaataaa ccatcctcaa taaaccgcag cgcagaggca atctcttccg
    56101 ggttatcgac cgtacgggtg acatcaataa aactgcctcg taccgctttt aacgtgtgtt
    56161 ctcctgacat cattgactcc tttactgtta atccatctct tccgctgagg taataccagg
    56221 cagaacgttt tcctgtcggt agccaccagg gaggataata tttaataaaa tcgcagttaa
    56281 cccgccagca caaatagggt tttcaactaa tacataaata gaggctggca atattttaaa
    56341 aatttcggga tcgtaggaga cgccaagtcc taaacctaaa gaagtggcga caataagtgt
    56401 ttctcggcgc tttaaaccgt tggtgatgat gatgcgaatc ccagcgatgg caatcatgga
    56461 aaacatcaac gtcattgcgc ctcccagaac tgccgaggga atggtcgtga agaagccgcc
    56521 aatcatcgga aacaaaccga ggataaccag cattaccgcg atggttcgcc cgacataacg
    56581 tgaagcgacg ccagtcatct gaataacccc attattttgc gcaaacgtgg ttaagggtaa
    56641 tgacccgaca gcggaggcga taacagaaac cagaccgtct gccagcacgc cgcctttcag
    56701 tcgggactga tactcttctc cctgaatggg tcggcgggaa accattgccg tggcggtgat
    56761 atcgccgaca gcttccagca cgctaagcag ataaatcgtg ccaaccacca ggaactgatg
    56821 gaagctaaaa ctaaagccgt atttgaacgg atgtgggatg gtgattaacg gcaaattgcg
    56881 catactgctg aaatccacca tgcccaggca taacgacgcg atataaccga cgcatagccc
    56941 aatggcgatc cctcccatgc gtagcaacgg actgcgacag cagttaaagc cgatcaccac
    57001 aatcaaaacc aataaaccaa cgccgagatg ttcgtaattg ccgaaggtac cgctgctttt
    57061 ggcagcaaat cctccaccaa aatcgataat gccgacttta atcaggctta agccgatcat
    57121 cagtaccaca ataccgctga cagtaggcgt aataacccga cgtaaatagg gcaagataaa
    57181 tgaagatccg acaaccagta atgcgccaac gaaggagacg ccgaggagcg acgacatgat
    57241 taactcttcg tgaaaaccgt cgcttttcat gctgctgccc agcgcaatca tgaccgtaac
    57301 aaatgaaaaa ttgactgact gaattgagag taggccagaa ccgacgatgc catagcggtt
    57361 tacttgtaac caggtaccaa taccagaggc gatcatcgcc attgaaacaa gataggcagt
    57421 tgtttcagcg gaaagctgta aggccgcccc cacgattaac gcgggggtta ccatcggaac
    57481 gaaaattgcc aacagatggg taatggcacc aacgagagcc tgatgaaagg gagggcgatc
    57541 ctccagttca aatataaggt cagaacctgc atggtttata tcagacatcc cttcactcct
    57601 tttaaagtaa atttgatcat ctgaattagt aaaataaatt cccacgaatt tggggcgcag
    57661 gacgccttga gaacggctta tctcattttc gcagtcacta tttagtcatc gtccaaacag
    57721 agaaacaggc cgccgaagcg gccatttatt atttatatct tttccagcgc ggtgagtatt
    57781 ttctccggtg tgaaatgcca ttcgcgtaac cagatgccgc atgcatcgtg aattgcggta
    57841 gcaatcgccg gagctgcgcc atttacaccg atttccgaga tcgatttcgc cccgaacggg
    57901 ccgactttat cgtcgctcgg caccagcact gcgcggaaat cacgcggaat gtcaccaatt
    57961 ttcggtgcgc cgtagctgcg taaatcacgc gttaacgggt gaccttcggc gtcataaatg
    58021 atctcttcgc tcatactgtg gccgatagct cgcagggtag caccgtagat ttgtcccaac
    58081 gctaactctg gattgaccgg tgtaccgcag tccagcaagg cgtagaattt atccaggcgg
    58141 atttcacccg tacgcgtgtt gacggcaact tcagcgaagt ttgcgccata cgggaaggcg
    58201 aaatcaggcg tgatataact gccagttccc accagtgaac caaagccggt gccggtttcg
    58261 cctttatggg caatatcccc gaaactaact tcgcctttct tgccgcgcac gacgcccggc
    58321 gttgctagtt gaacatctgc cactggctca cccaacattt gcgcgccgtg gaacagaatt
    58381 ttctcccgca gattttccgc tgccaaacgc gccgcgttac ccgagaagca agtaccggac
    58441 gaggcatatg cgcctttatc aaacaacgca tgatcggtat caccggagat aacatgcacg
    58501 tcctgcggtg ggcagtgcag cacttctgct gccagtttcg tcaccacggt atccagacca
    58561 gtaccaatat ccgcaccgcc agaatgaacg ataaaggtac cgtctgattc cagtttgatc
    58621 atgcagttag cctgatcgat atccgggatc cctgatttct gcatgataat ggcgacacca
    58681 cgaccgatgt gccagtcacc attttgtggt tttggtgaag accattggat catctcgcgc
    58741 ccctgacgca ggatctcttc cagtgcgcag ctggcggcgg aaggaacgga ggtcggcgct
    58801 ttaccttcac cgattgcacc gagaattttc agctcttgcc cttcgtgtac ccggttacgt
    58861 tcgataattt ccagttggtc gatctgtaac tgttcagcca gttccgctaa tgccatggtg
    58921 atagcgaagt tacctttcgg tgcgccataa ccctgataag caccatttgg gcaaatgttg
    58981 ctgtagtagg tggtgacctg gaaatcgacg ttatcgcacg gatataacgg cagcgacagc
    59041 gccggtccgt tacacggtac ggtgagtgag tggttgccgt aagggccagt gttggcgcgg
    59101 aaatccatct tcactgccgt caggcgacca tcttttttcg ctcccagttt gacggtgact
    59161 ttcgcgacgt gacgagaggt gttagcaata aactcttctt cacgggtgta gcggaacagt
    59221 accggacgcc cggtcacgca ggttgcccag gcgcacactt cttccagcag gatgtcctgt
    59281 ttggaaccaa aaccgccgcc aactcgctct ttaatgacat gaactttatg ctgtttcatg
    59341 tccacgaggc gcgcgacctg gcggcgtaag tgccatggta cctgggtgga ggcgtggata
    59401 accagacgat cgccgtccat ccgggtaaag cagatatgtg tttcagtcgg gcactgctgc
    59461 gcctgcgttg agttataggt tcgctcaatg atcacatcgg catcggcaaa gcctttgtcc
    59521 atatcgccaa tatgaccatg aatactagcg gcgatatttt tgcgagggcg agaaccgatc
    59581 gggaagttga tgatcatatg ctcgccgcgc tgggctgcat ggctgttatc gtcttccaga
    59641 gtatctggcg caccagcaac atacaccacc ggttcatcgt gcacgacagg cgcatcttcc
    59701 gccattgctt cgtcgatcga cattaccggc ttaagcactt catattcaac ctcgatgagt
    59761 ttcaatgctt cgagcgcaat ttcttcactt tcagcgacca ccgcagcaac gcgatcgccg
    59821 acgtgacgca tttttttgcc gaacatacgg cggtcaagcg gtgacggttc cggtgcgctc
    59881 tgaccccccg gggtatagta gatatcaggg caattcaggt gagtaataac gtgaacgacg
    59941 cccggtaagg cttcagcttt gctgacatcc agatgagtaa tcagtgcgtg agcgtgtggg
    60001 ctacgtaaca ttttaatgac gcaggcgtca gccgttacgc ggtcttcaac atagcagggt
    60061 ttcgcctgca ccattttcgc ggcatcagtt ttaggataat gcttgccaat gacttcaagg
    60121 tcgtcacgga aagtcggagc gatatcaatg gtggcctgcg gattattttt acgtgcaacc
    60181 gccagttcaa tgacctgata gtattgctgc cagccagcat cgcggctgaa caaaccagaa
    60241 agcgcgtcgt cgatctcttc gcgggtaggt gcggcgatgc gatcgagaag atcggtgata
    60301 atcagagctg cagctggatc gttataacca gactgcacca cgccaacatc aaccattgcc
    60361 tgttgaacca gacttaactc gttccatttg cccagagatt ctgcggtacg aatatctgcc
    60421 ttctctaact gtgcggcaat aagcaaggac gcgttaacga tattaccgtt aaagatgatt
    60481 gcgtcagaac cggcaaaccc gaaaccatca tcactgttgc gtacagagtg cattcccatg
    60541 ttaaacaaca gcttttgcac gttttcgcct ggattaacgg ttagctcctg aggcgcgcca
    60601 tttaaagtaa agtggatgat catacggctt cctccccagc ctgttggcag tcggcataca
    60661 ggtcggctac cagaactccc gtgatatagc gtttataggc cacgctgccc cgcaaatctt
    60721 cctgcgggaa aatggcgttg gcgacagcct gttcaagtgc attgccttcc agattttgtt
    60781 tttcgacatc atgcagacgc agtgctttac tggcgacgcc atccagcgca attcgcatac
    60841 cgtcgtggtc tgtcattgca acggctgccg tcacgacggt taaacctgcc tgagaacggc
    60901 taattttgcg ggtcgcacag gtgcgatacg gatctttaat gataatttcg gttagcaggc
    60961 gatcgcatgg gcaggccagg tagtcctcga ttgacagcgt ttcgccgttg ccaaaaacca
    61021 gttcagcatc cagtgccagc aggacgggaa gcagcactga ctcttcctgc cgggcggcga
    61081 tttcaccacc aatggtcgac tgattacgaa catggcgtga gtaaacaaaa ccgagggctt
    61141 cacgcagtgc tgcaggaata aatcgcgcat cacgcagtgg ctgcaagcga gacattgcgc
    61201 caatccgcag tgcaccgtta tcccagtcaa cccagtccag ttccagatcc tgtaaggaaa
    61261 tggcaatctt tttatcggta cgggttggtg tagcattgag tttgctgccc ccggcgaacc
    61321 agacggcttc atcctggtag cggcgcttca gttccagcgc ctgttcgaca gagtcgggcc
    61381 tgaaaaattg ttcaatcatc ttctttcctt atttaaccct cttcctgcgg gaagagggca
    61441 tctgtcattt atgccagcgc atccatccga cgccacatac tggcagcggc ttttctggct
    61501 tgtgcataaa tggaatcgca atcgaagtta aactgacggt cttcatagac catcacacca
    61561 ttgaccatca cgctgtgaac gctgcctgag cccataccga aagcgatatg cccggcgata
    61621 ttgtctgcca gcagcggcgt cggcgagttg taatcgcaaa tggttaaatc agctttgtaa
    61681 ccggcttcca gaagcccaaa tttcgcgcca aagttgcggc tcatcagttc gttaccgtta
    61741 gtcagggctt tggcaaaact gtcaggccac agcggaccac ccgcatcgcg atgtttaaag
    61801 aaggcaaatt tcatctcttc aaacatgtcc gaaccaatgc cgtccgttcc caacgccaga
    61861 ttgcggatgt cgctaaggtg atggttgtag ccgacatggt tgttcatgtt tgaacgggcg
    61921 ttatgcacca ggaacgcatc gcgctgattg agtagggcga tgtcatcttt cgacaagtac
    61981 agcccatgag cgaccagcgt tttgctatcg atgagatcga attgcgccag tcgtgccagc
    62041 aggtctttgc cgtaccagtg gtgactgtag gaaacgtcgt aaaggtcttc cgcagcgtga
    62101 atatgcaaac cacggcctgt ggcgttcacg gcttcacgta gcatctccag accggcatct
    62161 ggcacggtaa acggcgcgtg agcaccgata tgtgcttcca ccagatacgg ctcggttgct
    62221 gctttcttcg cctcatcaat ctgacgggcg aagcggatgt tttcttctac accttcctgc
    62281 aactctttga tgccgttgtt acggtcagta gtttcaaaac aggtcatcgc gcgcaggcca
    62341 acttttaaaa atgcgtcgcg caatgtggag agcgacccgc cgatatacgc cggagaggca
    62401 tggtgatcga taaccgatgt acatccgctc ttaatcgctt ccagggaaca aatcagcccg
    62461 ctgtaataga gcgactcttc atcaagggcg cgatcgagcc gccaccagag atttttcagc
    62521 gttgagatga aatctgggct gggggcgatg tttgccataa ttccgcggga aagccccgag
    62581 taaaaatggt tgtgcgagca gacaattccc ggcatcacaa tccgaccatg catctctttg
    62641 tagctggcat cggggtagcg ttgcgtcagg gcatcgccga tagcgacaat cacatcattt
    62701 tcgatagcga tatcaacgcc ttcctgcact ttcgccgggt gtaactgcac tgcggtgaca
    62761 ttcttcagaa tcaacatgat tacacctcca cgcggcccag cagataatga tgatgctggt
    62821 ggacatggct gatgatgcgg cacatatcgt tcagctccgg tggtacgttg ttgaactgac
    62881 cttcgctgtc gatgtttaac acccagcttt ggttattcag tcgtactcgt acccggcaat
    62941 cttccacaag gaagcctggg ttgctgctgt tatcaaagtc ttgcgccagg ctgaaaacgg
    63001 tgattttgtc tttgtatggt ttaccgttcc acggacagaa ctgagcgcag ttgccgcatt
    63061 cgttacagta agcgtcgagg tgcagcgtct ggaatctatt ctggaagcct gggaccgcaa
    63121 tggagacgtt ggcgcggttc gggcagacat ccacacactt gctgcaaacg tagttacatt
    63181 caaggcagcg agcagcttcc tgcgcgacaa acgcatcacg atcgtcactg ttcaccagcg
    63241 tgatagagat atcgccttta cgttgataga tttccgccgg attgacgttg ttccagtatt
    63301 tatcgttctg gtgggaacgg atattttccc gactaaggat ggcatcggtc gcccgacgcg
    63361 cggttccgac agcagcgaca atggaggatg gtccgcgctg tacgtcgccg atcataaaga
    63421 cgtcagtcag acgagtttcg ccattatggt cgacgtctgg ccagccgttt ttgtccagcg
    63481 gcacgcccat cgcattcagg gcttcagtat cctgctgttc accaatggcg gtgatcaggc
    63541 tgtctacatg cagtgttact gtttcgttgg tttcaaccgg acgacgacga cctttctcat
    63601 cgggttcgcc aagcgacata acgcgcaagg ttaaggtgcc atcagcatcg aaacgttccg
    63661 gattattcag gaaacggaac tccacgccgt cgtgcaacgc ttcttcatac tcttcgcgcc
    63721 atgcgggcat ctcttgcagt gaacgacggt aaacgaccgt tgctttttct acgcctggaa
    63781 cgcgtaacgc cgcacgagcg cagtccattg cggtgttacc cgccccgaca acgaccacat
    63841 gtttgcccag cttgagcgct gtacccttgt tgtattcacg caggaagggg agtgatttca
    63901 agacattttg gttgtcgccc gccagtttca caccgctatt tttatctgtg ccggtggcaa
    63961 tcagaacata gtggaagtcc tgatttttta actgctcaac ggttaaatcg ggtgagcagc
    64021 catactcaaa tttcacgccg tgagcggcaa caaaatcgat atcgtgctga attaactcag
    64081 ccggaattcg gaactgagga atgatatttt tcaccacgcc gcccgcattg gcttcgcgtt
    64141 caaacagcgt aaccggatgg cccgctctgg caaggaagta acctgctgcc agacccgccg
    64201 gacctgcacc aatcacggca accggatggc gtgaaccaga accggctggt ttgtgccagc
    64261 gttgcttata ttcatcccaa cctttttcca gcgcgacttt tttcagttcg cggatattca
    64321 gcgcactgtc gtaatccagg cgggtacagt tgtattggca ctggtgatcg caaatatgac
    64381 cggtaatggc gggcagagcg ttgcgttggt agatgagttc cagcgcgtcg gcatagcggt
    64441 gttcgccaag cagacggatg tattccggaa tatcttgctt gatagcgcag gcagtaacac
    64501 agggggcaac gtagcagtcg gtcagcggca ggtcttctgc cacttcaata cgctcttctg
    64561 gcttccagtg tttctgggtg tattccatgg ttaacgcatc tgctgccagt ctgttcagtc
    64621 gttcgacgtc aacatggtca agtccccagg cgtcggagcc ttccagctcg cgcatgcagg
    64681 cacttaagcg caaatagccg ccaggtttca gcaggtcggt tgccatagta atagggcgaa
    64741 tacctgtatc aaaaatatcg cggatagtca gctgactggc accaccggaa taagaaatgg
    64801 gcagtttgcc gtcaaaggcg cgagagagaa ctgctgcaac attgatggag agcgggaaca
    64861 gcgcacggcc tgacatatac atctcttcac caggcagtgc gcctttattg ttgatggtgc
    64921 cgagagtgtt agtcagtttt acgccaaagc cgagtgattt ttcttttgcc agtgccatca
    64981 ggcgttccag catttccagt gcttgcgtca gcttgaggtc gtgatcaaat gactcttctt
    65041 ttaagcctat gtaaccgaaa ccgcagacat cgaggatctc acgaacacgc gcgtacccca
    65101 gtaaggtcgg gttaagtttc acaaaggtgt tgagcccttt ttcttccagc atgtagcggc
    65161 aaatggcttc gatttcatgc ggaggacagc cgtgcatggt ggagagggtg acgccatgca
    65221 ccatactggt ggggatgcga gcgggtaagg cttgcaagct ttcgcgtttt tcctgcaatc
    65281 cgtggcgggc taaaaatgcg tcatcctgga gtaatttatt cagcgtatcg cgatattgcg
    65341 cgaatttcgg atggtcagat gcgtccatca tattgtcgat gaactgttgc attggcggtt
    65401 gcttaatacc ttcgaggttg taaccgacgc tcatattaaa gatgaacgat ttaccggaat
    65461 cagaaggttg gaacatcgct tcgagaaggt gcagggcaaa ccaggctttg aggtattcat
    65521 cccaggcttt aagcagggta aattcggtag accattcggt gttaaagcac tcgtcttcgg
    65581 catcaataca gggcttttcc agctccaggc ggtcaagaat ttggacggtt tttagttcga
    65641 tgaaacgtcc gccagtcagc caggaagtga caatattttg cgcgagctgc gtgtgcgggc
    65701 cagcggcagg gccgacggga gtggcacagg tttcaccgaa gacgctaaca gttttacctt
    65761 ttacaggtga gtaaaactgt tgctcgggaa taccaaagat tgagcgttgt tgttggtatt
    65821 catcaaatat gcgcgtcaaa agttcctcaa acggaatggg acgcataata tcccccataa
    65881 ccctctcctt agcttataca gatatcattg agtgaccggt gttgagaaat ccggtggttt
    65941 agggggatag agcaacaatc gcaccactct tgccaggttt ggcgggaaat ataaaaaaat
    66001 gtgaggcggc tcgaaactta agggcttttt gtgaatgaac tcacaggtgc taaatgagaa
    66061 ttaaaatcac tcctgaaaat tattattgcc atcccatata tcactttttc tcttttctca
    66121 aaatcagtga ttccttatca ttatgataaa gagtgctgga gggattaaat atttgaacgc
    66181 aatcgtttaa ctacgttttt taaattttag tatacctttt ttgcgctgta ataaagtcgt
    66241 ccggagtatc aatatctaaa attatttctg cattttctac ttcgacggaa taatgagctc
    66301 ccataaggag tgcctgacgc atattggtga tattggggcg cttgattgcc tgcatcaggc
    66361 atggttttga cactaaaatc ggatggccgg ggatgccatt atggagtggc agtattgcgc
    66421 catcatttcg taacgaccag atttttctga aaatatcgat ggtgagggtt ggcatgtcac
    66481 cgtgggtgag aaaacaatgt tctgtttgca ctgcgggtac tgcggccttt actgatgtta
    66541 gtaaaccctg cgcataatct gggttgtgga taatagtgat attgctctgg ttcgcataac
    66601 gttcgtgcag ttcattacca cgatagccgg tgactaaaat aattcggcta caaaactgca
    66661 acgcattttt gatacttgta tcaagaattg tcccctgttg ccagggtaac atcattttcc
    66721 attgccccat tcttgatgat aatccggcag cggtaattat acagtcgatg gctgacatag
    66781 tggttccatt atttataggg gctaataacg cctgataatt gcgttacttc ctgatggatt
    66841 aagttgggga taatttatcc cagagaggtc ataaagactc gtgaaaagta tagttgaccc
    66901 atcggcatta ttcattgatt taggtgcgca gaaacgtccc actgtgattt ctgttgttgg
    66961 tgctgggggg aaaaccagct tgcttttttg gctggcagaa ctgctccagg ctagcggcag
    67021 gcgtgtgtta atcacgacga caacacatat gtttatgcca acgtctcact ggcccgtggt
    67081 tttctgtcgt gatcccgcca tgcttcctca tgcgtctctt acatccccca tttcattttg
    67141 ttttcacagc tggaaagcga acctgggaaa agtgcaggga tttacgccag aagcgattga
    67201 tgcactggtg caacgaccag agtgtgacgt aattctcatt gaagcagatg gctcgcgtgc
    67261 aatgccgtta aaagcgcctg atgagcacga accttgcata cccaaaagca gttgctgcgt
    67321 gattgctgtg atgggaggac atattttggg cgcgaaagtg agcacggaaa atgtccatcg
    67381 ctggtcgcag tttgctgata ttaccgggtt aacacctgat gcaaccttgc aactgagcga
    67441 tctcgttgcg ctggttcgcc accctcaggg ggcgtttaaa aacgtaccgc aaggttgtcg
    67501 gcgagtctgg ttcattaacc gtttttctca atgtgagaat gcgattgcgc aaagcgagct
    67561 ccttcaaccg ctgcaacaac acgacgtaga ggcaatctgg ctgggcgata tacaagaaca
    67621 tcctgcaatc gcgcgcagat ttgtgaatta gcggcccggg tattccgggt tactgaaagg
    67681 atatccattg aggttggtat gaatattttc acagaggctg caaaactcga agagcaaaat
    67741 tgtccgtttg ctatggcgca aattattgat agccgaggct cgactccccg ccattctgca
    67801 caaatgttag tgcgcgccga tggctctatc gtcggtacaa ttggtggcgg aatggttgag
    67861 cggaaggtga ttgaagagtc gcttcaggca ttgcaggaac gtaagccgcg attattccat
    67921 ggacgtatgg ctcgtaacgg tgcggatgct gtcgggtcag attgtggagg tgcgatgtca
    67981 gtgtttatca gcgtccatgg tatgcgtcca cgtctggtgt tgattggcgc ggggcatgtc
    68041 aaccgggcga tagcccagag tgcggcgcta ttaggatttg atattgccgt tgccgatatt
    68101 tatcgcgaaa gcctcaatcc tgaactattc cctccatcaa ccacgcttct ccatgctgag
    68161 tcgtttggtg cggcagtgga agcactggat attcgccctg ataattttgt cctgattgcc
    68221 acgaataatc aggatcgtga agccctcgac aaactcattg aacagcccat tgcatggttg
    68281 gggttgctgg caagtcgtcg caaggttcag cttttcctgc gtcaattgcg tgagaaaggc
    68341 gtggctgaag aacatattgc ccgtttacat gcgcccgttg gttacaacat aggtgcggaa
    68401 acgccgcagg agatcgccat cagcgtgctg gcagaaatat tacaggtgaa aaataacgcg
    68461 ccgggtgggc tgatgatgaa accttctcat ccttccggac accagctggt ggtgattcgc
    68521 ggtgcgggag atatcgccag tggtgtggcg ctacgtctgt atcatgcggg ttttaaagtg
    68581 atcatgctgg aagtggaaaa accgacagtg attcgttgta ccgtggcgtt tgcccaggcc
    68641 gtgttcgatg gcgaaatgac ggtcgaaggc gtcactgctc gcctggcaac cagctctgcg
    68701 gaagcgatga aacttaccga acgcggattc atccctgtga tggtagatcc cgcctgttca
    68761 ttgcttgatg aactgaaacc gctttgcgtg gtggacgcta ttctggcgaa acagaatttg
    68821 ggaacacggg cagatatggc accagtaaca atcgcgcttg ggccgggctt tgctgcaggg
    68881 aaggattgtc atgcggtaat tgaaacaaat cgcgggcact ggctcggtca ggtgatttac
    68941 tgtggttgtg cgcaggagaa taccggtgtt cctggcaata ttatggggca taccacccga
    69001 cgggtgatcc gtgctcctgc tgcaggcatt atgcgatcca acgtgaaatt aggcgatctg
    69061 gtgaaagagg gcgatgtgat tgcctggatt ggtgagcatg aaattaaagc accgttgacg
    69121 gggatggtgc gtggcttgtt gaacgacggc ctggcagtgg ttggtggttt taaaattggt
    69181 gatatcgatc ctcgtggtga aaccgctgat ttcaccagcg tttctgataa agcccgggcg
    69241 attggcggcg gcgtacttga ggcgttaatg atgttgatgc atcagggcgt gaaagcgaca
    69301 aaagaagtgc tggaagtggc ttaaatgaaa atggccggat ggtattaccc acccggcctt
    69361 aaaattactg acaaatcacc gttccggttt tgccttcaat cccttctttc gctttgctca
    69421 atacggtaat cagtgcttca cgacctgcgc gagagcgagc aaacgaggcg gcagcttcca
    69481 ctttaggcag catagaacct ttcgcaaaat gaccttcttc gataaagcgt tcagcatcac
    69541 ttaatgacag gcgatcgagc cactgttcat tctctttacc gaagttaatg gccacttttt
    69601 ctaccgcagt cagaatgatc agcatatcgg catcaatcat ttctgctaaa cgggcgctgg
    69661 cccagtcttt atcgataacc gcgctggcac cacgcagatg gttaccttca cgaataacag
    69721 gaataccacc accgccaacg gtgatcacca cctggcctgc ttctaccagc gcttttaccg
    69781 tctctttttc aatgatatca accggttttg gcgaggcaac aacacggcga tagccgcgac
    69841 cagcatcttc tttcagagtg taaccctgtt ttgtcagttg ctctgcttct tgctcggtaa
    69901 agaacgaacc gatcggcttg gtcgggttga ggaatgccgg atcgttagcg tcgacttcga
    69961 cctgagtaac cagcgttact acaggtttat taatgccccg agaaagcagt tcttctctta
    70021 aggcgttttg tagatcgtaa ccaatataac cctggcttaa ggcaacacag acagacatcg
    70081 gcagcattgg cgaatgcgct tcagtttttg cggcagcttc aaaggcctgg ttaatcatgc
    70141 ccacctgcgg cccgttacca tgagtgacga taacttcatg tccctgggca attaaatcaa
    70201 caattgcctg agaagtaatt tttaccgctt tcatttgtcc ggccaggtca tcgcccagcg
    70261 cattcccgcc cagggcgaga acaattttct tactcatttt tcctcactca tcattcttga
    70321 ttgtgtatag atccctggca gtggttagag cacgggaggg acaaacggtt tgcgtcgcag
    70381 gaaacgacct cgcccggctt tgcctgtaaa ggtgccgtca cagaaaatcg tttcaccacg
    70441 ggataaggtt ctgacaatcg cgccctgaca ggtaaaaccc tcccatggcg agtagtcggc
    70501 gttgtcgtgg agatggcgat gctgaatttg ttggctctga cgtgggtcga taatcaccac
    70561 gtcgccatcg gaaccgggcg ctaataatcc tttttgcggc cacaggccaa acagcctggc
    70621 gggcattgcg ctggttaatt caacaaagcg ttccggtgtt atacgtcccg tcatcacgcc
    70681 actggaaaac agtaactgca tgcggttttc cacaccgggt aagccatttg ggcagcgact
    70741 gaaatcgcct ttagaaattt gcaggcgttg agccatcgag aaggtgcagt gatcggtcgc
    70801 caccacgtca atcgcaccat cgctgatgcc acaccacagt ttgtcctgct cgcgtacgtt
    70861 acgcagcggt gggctaagaa tgaacttcat gccgtcttct gtatcgtaac tgcgttcgtc
    70921 caacaggaga tattgtgggc aggtttcaac ccagactggc tggtgattcg cacgggcaag
    70981 acgcagataa tccagaccta agccgttaga caggtgcacg atatagagcg gggcgttacc
    71041 ggcaatttgt gccaggttaa tcatgcgggc gatggcttcc gcttcgcatt ccagagggcg
    71101 actcaaggca tgatagcgcg gcgcggttaa ccctgcggcg ataaattccg cccgcttgct
    71161 ggcgatagcc gcatcatttt ccgggtgcac ggtggtcagc gcgccggatt catgcagacg
    71221 gcgtaatgcc tgcaaaacct cgtcatcgtt gagtttgtat tgataggtta aatagagttt
    71281 aaaactgctc agcccttcct cgaccatcat cgggatttcg tcgaggatgg cgtgattaat
    71341 gtgctggatc acaccgtgaa agctgtaatc gatgaccgct ttatgggcgg cataaccacg
    71401 ataaacctcc agttgatggc gtaaccgaca gccgtttggg ccaaatccca tatggtcaat
    71461 aatggttgtt gtaccgccac acgcagctgc gcgggtaccg gtaaaaaaat catcacaact
    71521 gcgcgcgatg ccgacatcaa tattgaaatg cgtatggaca tccacgccgc cagggaaaac
    71581 gtaacagcca gtggcatcaa tttcttcata cgggagctgc ggcgaaatat tgttgcccaa
    71641 ctggcgaaca atcccgcttt caatcagcaa atcctgtttg gcttgtccat ctgcgttaac
    71701 gacagtgccg tttttgatca atacgcgcat agcaaactcc aaaaaccctc cggcgaaccg
    71761 gaggggaata agtgaagagt aattattcgg ttgccagcca gcttaacggg attgcagcgt
    71821 acatcgcggc acaggtcacc aggtgagatt tccaggtttt ttcgttaggt gcatgcgctt
    71881 caggttcttt acccgggcca aagccgatca ccggaatgcc gtgacggccc atgatagata
    71941 cgccgttagt tgagaaggtc cacttatcaa caaccggcgc tttgccaaac agcccttcgt
    72001 aggcattcac cagtgcttta acggtgaagt gatcttcttc cactttccag gtcgggaagt
    72061 agcattcggt tgggtaaacc aggccagtcc aggacggacg gtcgtagttg tacatagaaa
    72121 caaccgcgtt agctttctgt actgcaggca gggcgcggat ttcgtccagc gcgccttccc
    72181 aggtttcgcc ccaggtcaga cggcggtcaa tagagacggc gcagctatct gctacagcgc
    72241 aacggcttgg ggatgtgaag aagatttcag aaacggtgag ggtgcctttg ccgaggaatt
    72301 cgtcataacc cagacgttgg gagagttctt gtaattcgcc aagaatcgga cccattttga
    72361 aaatggcgtt gtcaccgcgt tctggcgcag aaccgtggca gctaacaccc tgaacatcaa
    72421 tacgaatttc catacgaccg cgctgaccac ggtatacctg gcagtcggtt ggttcggtac
    72481 tgaccacaaa ttccgggcga atgccggatt gttcaataat gtactgccag cacagaccgt
    72541 cgcagtcttc ttcctgcaca gtaccggtaa ccagcagggt atattcatct tccagaccga
    72601 ggtctttaat gattttaccg gcataaacca tagatgccat gccgccttcc tggtcggaag
    72661 taccgcgacc accgatcagc tcatcagttt ccatgccttc gtacggatcg aagtcccagt
    72721 ttttgatgtt gccaatgccg acggtatcga tatgagcgtc cattgccacc agacgcgggc
    72781 cgtggccgat ataaccgaga acgttaccca tcgggtcgat ttcaacttta tcgaagccga
    72841 ctttttccat ctcttcttta atacgatgta ctacccgttt ctcgtcgcag ctttcactgg
    72901 gaatagcaac catgtcgcgc aggaagcgag tcatatccgc ctggtaatct tttgcttttt
    72961 caagaatcag tttgaatgga atattcttag ccattatttt tctccagatt ttttactccc
    73021 cgttttgcag ggagcagttc caatagggtg attaaggtgc tacagcgtgt ttgccttccc
    73081 agacaacttc gcggtagtgc ttcacgtcgg tgtcgccttc agtgctgata accagcacta
    73141 cggcatcttt gttcagcgcc agtttttcca tcaggctttg acgttgcggg tgataatgaa
    73201 ccgctgcgag aacgcccaaa ccgacagcgc cggattcacc ggagatgatg cgcgggtcgt
    73261 tgccgtacgg attacccagc acgcgcatac ctaatgcggc aacgctgtcc tggcaggaga
    73321 tgaattgggt ggcgcagtta cgtaggattt cccagcccag cgggttaggt tcgccacagg
    73381 ccaggcctgc catgatggtg gccatatcac cgccaacgtt gacgatgtcg cctttgacgc
    73441 cggagcgata aatacagtca gctttgtcag gttcaacaat aatgctgtgc agattttgcg
    73501 ggctatagac gtcgaccaga taacccagca caccaccggc cattgctccg acaccggctt
    73561 gcagcagaac gtgcgtcggg gttacgccca tttcacgcat ttgctcgacg gcttcatctg
    73621 ccagggttgc gtagccttgc atgatccagg ttgggatttt ggtgtaacct tcccatgccg
    73681 tgtcctgtac cacttcccag ccgtgctgct gcgcgtgttg catggtcagg cgaacggtat
    73741 cgtcatagtt catatctgtg acgatgcact cggcaccgag gttcagaatg gcgtcaacgc
    73801 gttcctgagc agaacctttc ggcatgtaaa tcaccgcatt ctgtccgagt tgctgcgctg
    73861 cccacgccac accgcgcccg tggttgccgt cggtggtggt cgcgaaagtc attttttcgc
    73921 cgatggcatt tttcaggtgc tcaaatgaca gcgtttcgat atcaagatga tatttttcac
    73981 acaataattg agcgatagcg tacgcaccgc cgagcatttt gaaggcgttc agaccgaatc
    74041 gttttgattc gtctttgaca agaattttct tcaccccaaa aaggtttgcg agatcgtcca
    74101 gcgcacaaag cggtgttggg cgataaccag ctattttctg gtggaactgg cgcgccagtt
    74161 tggcctggct ttgcgaaaag agcggtgatg tttcgccgtt gaaaaatttg ttatcggcga
    74221 tatcaatctt caatgagaaa acggacatat cctatccttt tggcctctgg aaatagaggg
    74281 ccgaccagct gatcggcccg aggggttatt tcacgcgttc ttgcgcttct ttcagcagtt
    74341 gctcgagcag tgcacctggt ttggcgtatt tacgggacag gatcatcgcg gcgatgatat
    74401 aaggcttcca gctggcttct ttgtaggtag cgatacggta tttttcgaat acgccttcag
    74461 tcacttcccc ttctttgcag gatacgccgc tgatatcagc tggcaggcag tgcatgtaca
    74521 gggcttcgcc atcacgggtc agttccatca tctcttcagt acaatgccag tctttgtgtt
    74581 gcgcgttctg tgccagacac tgtttttcca gtgcttttaa gccttcgtga tcgttagcgc
    74641 gcagcaattc ggtacgctgt tccatcactt tgtaaggtgc ccatgacttc ggataaacga
    74701 tgtctgcatc tttgaacgct tcttccatgc tggtgacctg acggaagcta ccgccagatg
    74761 ctttagcatt gtttttcgcg acttctacta catccgggat caggtcgtag ccttccggat
    74821 gggccagggt gacatccata ccgaagcgag tcatcagacc gatgatgcct tgtggtacag
    74881 agagcggttt gccatagctt ggagagtagg cccaggtcat ggcgattttt ttacctttca
    74941 ggttttccag tgaaccaaag tgttcacgta accatgccag gtcagccatt gactgagtcg
    75001 ggtggtcaat atcgcattgc aggttcacta aagccggacg ctgtggcagt acaccctgct
    75061 tgtaaccgtc atcaagtgca gcgccaactt cacgcatata ggcgttgcct gcgcccagat
    75121 acatatcgtc gcgaatacca atagcgtcgg cgcagaagga gatcatattg gcggtttcac
    75181 gcacggtttc gccgtgagcg atttgtgatt tgccttcatc gagatcttgc tgtgcaaggc
    75241 cgagcaggtt aagcgcggaa gcataagaga agcgggtacg agtggagttg tcgcggaata
    75301 cggaaatacc taatccacta ttaaagactt tggttgagat gttttcagca cgcagtgctt
    75361 ttaatgctgc ggcaacgtcc agtacttgtt tcagttcatc tggagtctgt tcccacgtta
    75421 acaaaaaatc tttctcgtga aggtgagagg tcagcgaatt gatatcctta atcagctcat
    75481 taacagtttt catcataaag tcccggtagt ggaatgacag gcaaagtggt ttatttcact
    75541 gtgacttgcc aaagtgattg gtgttttcac agctatacct gggactaaca ataagtgtgc
    75601 cagaattatc cacggggctg caaaagacta atttgacgga taatgtgtgg ggttgatcac
    75661 aaattgatgc cagcttccgg ttgtttgtaa aatacgaaaa cgtttggctt tttttatcgc
    75721 gttatttatt tgctgctaag tatcagaaat aagataaagg gacaggctat ccctcgtcag
    75781 tttgataaac tcaccgccat aattctcata ttgatataga aatgtgatat ctcttccata
    75841 atttcattag aaagtgataa aaatcttatg tttttatcaa agccaatttc gcctgagtta
    75901 atatgatcat gatctgtgaa ccatcaacgt cttcaatgat cgacttttaa gtaagtctgg
    75961 aaggtaaagc tatggagctt gctactacgc agtcagtatt gatgcaaatt caaccgacaa
    76021 ttcagcgttt tgccagaatg cttgccagcg ttttgcagct tgaggttgag atcgttgatg
    76081 aaaacttgta tcgcgtcgcc ggaacgggcg cgtatgggaa gtttcttggt cgccagttga
    76141 gcggcaactc acgcctgctc tgccacgtcc tggaaacgaa aactgaaaaa gttgtgacac
    76201 agtctcgctt cgatcccctt tgcgaaggtt gcgatagtaa agaaaattgc cgcgaaaaag
    76261 catttctggg tacgcctgtc attttacagg atcgttgtgt tggggtgata agtttgattg
    76321 ccgttaccca cgagcaacaa gagcatatca gtgataattt acgcgaattt tctgattatg
    76381 ttcgccatat atccaccatt tttgtttcga aacttctgga ggatcagggg ccaggagata
    76441 acatcagtaa aatattcgcg accatgatcg ataatatgga tcagggcgta ttagttgttg
    76501 atgatgaaag tcgggttcag tttgttaatc agactgcctt aaaaacactt ggtgttgtgc
    76561 aaaataatat tattgggaaa cctatccgtt tcagaccatt aacatttgag agtaatttta
    76621 cccatggaca tatgcagcat attgtttcgt gggacgataa aagtgaatta atcattggtc
    76681 aattgcataa cattcagggc cgacaattat ttttaatggc gtttcaccaa tcgcatacca
    76741 gtttttctgt agcaaatgca cctgatgaac cgcatattga acaattggtt ggcgagtgcc
    76801 gtgttatgcg gcaattaaaa cgactcatta gccgtattgc acccagccca tccagcgtta
    76861 tggtggttgg tgaaagcggc acgggtaaag aagttgtcgc ccgtgcaatc cataagttga
    76921 gcggaagacg gaataaaccc tttattgcta tcaactgtgc cgcgattccg gagcagcttc
    76981 tggagagcga actgttcggt tatgttaaag gcgcatttac tggcgcttct gccaacggta
    77041 aaacagggtt gattcaggcg gcgaatacgg gcacgctgtt tctcgatgaa ataggtgata
    77101 tgccattaat gttgcaggct aaattactgc gcgctattga ggcacgtgaa attctgccga
    77161 ttggtgccag tagcccaata caagtcgaca ttcgcatcat ttctgcaact aaccagaatt
    77221 tggcccagtt cattgccgaa ggtaaattcc gcgaagatct cttctaccga cttaatgtta
    77281 tcccgataac tctgccaccg ctgcgtgaac gtcaggaaga tattgaacta ctggtgcatt
    77341 actttttaca tctgcatacc cgtcgtctgg gatcggttta tcctggcatt gctcccgatg
    77401 tcgtcgaaat attgcgtaag catcgttggc ccggaaacct gcgcgagtta agcaatttga
    77461 tggaatatct ggttaacgtt gttccttcag gtgaagttat cgacagcacg ctattgccgc
    77521 caaatctgct gaataatggc acaacggagc aaagtgatgt aacagaggtt actgaggcac
    77581 acctgtcact cgatgatgcg ggcggcacgg cgctggagga gatggaaaag caaatgatcc
    77641 gcgaggcgct ttcacgtcat aacagcaaga agcaagttgc tgatgaactg ggcatcggca
    77701 ttgctacgct ctatcgcaag attaagaaat atgagttgtt aaacacataa catcagtgaa
    77761 tccggcctga attcaggccg gatatccttt tacttcgttt tctcgcaatc cagaactgta
    77821 tttacaatca tctgataccc cgtgcagcga caaagatttc ccgccagtcc gcgacgaatt
    77881 tccgtaatgg ttaatggctt ctcgcgtggt ttcgccagca ttgccgtggt agccataatc
    77941 aggccaggcg tacaaaaccc gcactgcact gcgccggatt tcgcataagc ctgctgaaca
    78001 tgagaaagtt ttccgccttt cgcttcacct tccagcgtgc ggatctcttt tccttcagcc
    78061 caggcggcaa ggtataagca actgtctatt gctgtgccgt cgaccagcac cgtacaggca
    78121 ccacattcac ccacgcagca cccttgtttg acacttagca gcccttgttc gcggagtaat
    78181 tccgagagcg gcgtgcctgg tacggcatga agctgaaaag gcatcccgtt aatggtgcat
    78241 tcgatggtaa ttgtttcgct gtgattcatt gcaattttcc ccccgccgcg gcgacggctt
    78301 cgctaatcac ttttttggtc atcgtctgga tgagatgcag acgaaactct ttactggccc
    78361 gccatgaaga acgcggggcg acatcttgca ggacagattc gctgatagct tccagcgttt
    78421 gcaggtttaa tggcgcattt tgtgcagtct gttcggcatg ttggcagcga atcggcgttg
    78481 gcgcggcaac accaaatgcc aggcgtaatt cgctgaaatt gccgttatcc agtcggcaat
    78541 gtgcggcaca gccaatcgtt gaaatatcca ttgcgtcgcg catggcatat ttaaaatgcg
    78601 cgctgcccac gtgttctttc ggctggggcg gaaaatgaaa ggcgacaagg atttcgtcat
    78661 gctcaagaga cactttgccc ggcccggtgt gaaagccatt aatcgggacg aaacgaacac
    78721 cgcgtgggga gtggatctcc agtttcgcgt cataaatgag cgttggcgtg gcagaatctg
    78781 cgctggtggc accgttgcaa atatttccac cgtaggtagc gacgttacgg atctgcggtc
    78841 cagcaatgga cgaggccgca gcacataacg ccgggagatg acgttgagtt atagaatctt
    78901 ctattagctg ggtaaatgtc gttgcagagc cgatacgtag cgagccatct tccgccagcg
    78961 taattccccg cagctccgcc agattatgaa tatcaacaat atggcgataa cggtcattgt
    79021 gatggtggag ctgaatcagt acgtcagtgc caccggcgag cagtttggcc tgcgggttgt
    79081 cagccagcag gttgatggca tcggcaaggg ttgctgcgcg atggtaagaa gcaaaatcaa
    79141 acatgatgtt atcctcaaat caatcctgcc agatggaact cttcatataa ccgttttggc
    79201 gtcagcggca gtgtattgat tgcaacaccg gtagccatct tcaccgcgtt acgaatagca
    79261 gcggcaacag gaattattgg tggctcaccc agtgacttat gtccgtatgc ggattgcggc
    79321 tcattgattt cgacgaacgc gctttccagt tgtggcagat ccggcatggt cggcattttg
    79381 taatccagca gattggggtt acggaccacg ccgcttttcg catcgatgat catctcttca
    79441 aatagcgccc agccaatgcc cattcccatt ccgccgtgta cctgaccttc tgccagtagc
    79501 ggattaagaa tatggcccga atcatgaacg ttgaggatgc ggttgatggt gactttgcac
    79561 agcgcaatat cgaccgtcag atcaacaaag gtacagccga acgccggtgg gttagtggtg
    79621 gttttgatgg agctttcagc agagagctgc ccgccgcgtt cagggtggta gaaagcgtcc
    79681 atcgccaaat cttttaacga cattaacggc tcttccggtc gttcaaccag cacgatatgg
    79741 ccttttatca gggtcagatt catcgctgac tgatgtagca tgactgcggc gtgagcgatg
    79801 attttctctt ttaataacag tgccgcactg cgcagcgcag gcgcggcaac atagctctgg
    79861 cgtgaggcaa atgcgccggg atcgaacggc gtaacgtcgg tatcttgtgt tgaaataacg
    79921 cgaacgtcgc tgaccggaac ccccacggtt tctgccacca tttgcgagaa gacggtgtcg
    79981 gcaccctgac cgatttccgt cgcgccgctt tgcacgttga tggttccatc ctgattcatc
    80041 agaaggcgcg cgcctgctat ttctacgccg acaggccagg tgttagaggt gtagctaaaa
    80101 caggcgacgc caacgccgcg gcgtaaattg ccttgctggt tctggcactc tgcacggcgt
    80161 ttttcccatt caaagatttt ccggcctttt tcaagacact ccggcaaccc tgcgctgtaa
    80221 atacgtttgc ccgtgagcgg attagcatct ccttcgcgtg aggcgttgcg taaacgaatt
    80281 tcaacaggat caatacctaa cgctgtcgcg gcgtcatcaa gcatagactc aacggcaaat
    80341 acgacttgtg gcgcgccata accacgcatc gcaccagccg aggggaggtt ggtatagcag
    80401 gtctttgaac tgtaagcgta ggcacaacga ggataaaggt aagcgacttt atttccccct
    80461 gcagaagcga tggagtgccc gtgagataca taagcgccgg tgttagacag aacatccaga
    80521 ctataacctt tcaatgttcc gtcgcggttc acgcccattt gcccgtcaat agtaaaagcg
    80581 tggcgggtac gggttgcgag gaaacactct tcacggctaa gggaaacttt caccggaatg
    80641 ccgccaagct tgctggtcag gaatgccgcc attggctctt ccagtacatc ctgtttatta
    80701 ccaaaaccgc caccgacaaa tggtttgatg actcgtacgc atgaccaggg aatatccagc
    80761 gcctgaccaa ccacgcggcg aacaatgtgc gggatctggg tgctggaaac gatggtaatt
    80821 cgcgagtcat cctccatcca cgccagcgat gttacgcttt ccatgtgaca atgttgaata
    80881 acgggggtct gatagtgccc ctgtacctgg tagtcggcgg catcgattgt ttgttggaca
    80941 ttgcccgtcg acatcgtgct ttgtttcagt aaattgccgc cgttatggat tggtgcagcg
    81001 tcctctgcca gcgccgcttc tggcgtggtg ataacgggta attcttccca ctcaatgctg
    81061 accaattgcg ccgctttttc tgccgtgagt tcatcgcggg ccacgacgat ggcaacggcg
    81121 tcaccatgat gacgaacatg gcgagttagc agtgcgcgat cggcggtatc gcgcttgttt
    81181 tcgtcaagtg tccatgcatg cccagctgta gcgaatggga tatcaggcac atcttcccag
    81241 gtaaaaatcg ccagtacgcc tggtaaactt ctggcttgtt catcattaat acttacggca
    81301 taaccatgtg cgatagggct acgtacatat ttcgcataac acatgcccgc cataacataa
    81361 tcgtcagtat atcgtgcccg cccggtgacc ttagcaatgg catcaacgcg catgcatgat
    81421 tcacccgtag cggttgcttc ccgcgcttcc atagaaatcc cctcgatttg aacatttggt
    81481 tcttatggca aagatttagt ctaagcaggc aagatttacg ccagaaacgg cgataagcgt
    81541 taagggagga aggaagtgtg atgatgttaa aaaaacacag atatgatacg aaacaggacc
    81601 ctgaataggg gtagtgatac gtatcaatat gagtggcgat aaaatcaatg tgataaatta
    81661 ggattattac gcatcaggat gggaaatagg gtaaaaaagt caggaacttc gatacctcta
    81721 cagtgaatat tttgctgatg atacttccct ttgccaaatg aaattaacct ctttctctat
    81781 tacatcgttt ttccagaagc aagagattgg gtatcaagag gctggctgct atgatatggc
    81841 gtcttgtttt ttaagcgagg aaagattttg agtgcgggac gcctgaataa aaaatctctg
    81901 ggtatcgtga tgttgttatc ggttggactg cttttggcgg gctgttcggg tagcaaatca
    81961 tccgatacag gaacgtattc cggctccgtt tacaccgtga aacgggggga tacgctatat
    82021 cgtatttcgc gcaccacggg aaccagcgta aaagagctgg cgcgactgaa cggcatttcc
    82081 cccccttaca ccattgaagt tggtcagaaa ctaaaactgg gtggggcgaa aagtagcagt
    82141 agtacacgta aatcaaccgc caaatcaacg accaaaaccg catcggttac accgtcatca
    82201 gcggtaccga aatcttcctg gccgccagta gggcaacgtt gttggttatg gccaacgaca
    82261 gggaaagtta tcatgccgta ttcgacagca gatggcggca ataaagggat tgatatctca
    82321 gctccacggg gtacacctat ttacgccgcg ggtgcaggaa aggtggtgta tgtgggcaac
    82381 cagctgcgtg gctacggtaa tctcatcatg attaaacaca gtgaagatta cattacggct
    82441 tacgcccata atgacacgat gctggtaaat aatgggcaaa gcgtgaaggc tgggcaaaaa
    82501 atcgccacca tggggagcac ggatgcggca tctgttcgcc tgcatttcca gattcgttac
    82561 cgtgcaacgg caattgatcc gctacgttac ttgccgcctc agggcagcaa gccaaaatgc
    82621 tgatggcgaa ttaatcagca gtcagcagcg cggcacttgc taagaagagc gtaaggttta
    82681 taatgcctta cgcatctcga agcgggcgta gttcaatggt agaacgagag cttcccaagc
    82741 tctatacgag ggttcgattc ccttcgcccg ctccaattta tcttcacctc atcaatctgc
    82801 catttttgct gtatctgatt ttctatatta tcttaattca atcgcttcag acttttcgca
    82861 atctctgtaa cactccccca tttttttgta aataaaaagc gtttacgcaa tgcaatcctt
    82921 ttgtaacaac ccaggtaaaa taccttattt tcaagttgtt aaataagtgg ttatctacgc
    82981 ttttaacaaa cgattgatgg aatattttat gaaagggaaa tctgcattaa ctcttctgct
    83041 cgctggtata ttttcgtgcg gcacatgcca ggcgacaggg gcagaagtga cgagtgaatc
    83101 tgtatttaac attcttaatt ccacgggggc agcaacagat aaaagttatt tatcgttaaa
    83161 tccagataag tatcctaatt atcgtcttct gattcactca gccaaattac aaaatgaaat
    83221 aaagagccac tataccaaag acgaaattca aggattgcta acattaacag aaaacacacg
    83281 aaagttaacg ttgacggaga aaccctgggg aacgtttatt cttgcttcta ctttcgaaga
    83341 tgacaaaaca gcggcagaaa cccactatga tgctgtctgg ctgagggata gtttatgggg
    83401 ctatatggca ttagtatcag accagggaaa tagtgtagcc gcaaaaaagg ttctgctcac
    83461 cctgtgggat tatatgtcta cgcccgacca gattaagcgg atgcaggaca tcattagcaa
    83521 cccaaaacga ctggatggta ttcccgggca aatgaatgct gtgcatattt gacagcaact
    83581 cccccgtgat ggcagatgtg caggaagaag gcaagccgca gctgtggaac cataaacaaa
    83641 atgatgcgtt aggtctttat cttgatctcc ttatacaagc aatcaatact ggtactatta
    83701 acgctgaaga ttggcaaaaa ggtgaccgac taaaatcggt
//
LOCUS       DAZKIP010000007        80276 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZKIP010000007 DAZKIP010000000
VERSION     DAZKIP010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 80276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.007"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(9..2321)
                     /gene="pbpC"
                     /locus_tag="ACHOUK_000556"
     CDS             complement(9..2321)
                     /gene="pbpC"
                     /locus_tag="ACHOUK_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311412.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFP9896068.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEVA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEALPLPDGALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGEIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIASVHFSFQ"
     gene            complement(2322..7283)
                     /gene="yfhM"
                     /locus_tag="ACHOUK_000557"
     CDS             complement(2322..7283)
                     /gene="yfhM"
                     /locus_tag="ACHOUK_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFP9896069.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSAPERQKLAQQSAGKALTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKAGYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADEFGSNESKVIVAAPVIAELNMPRF
                     MASGDNSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRDEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYSLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKAQNSNLNSDQLATLQVTNSGEQPLWLRVDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            7490..8335
                     /gene="sseA"
                     /locus_tag="ACHOUK_000558"
     CDS             7490..8335
                     /gene="sseA"
                     /locus_tag="ACHOUK_000558"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFP9896070.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPLK"
     gene            8678..8803
                     /gene="timP"
                     /locus_tag="ACHOUK_000559"
     CDS             8678..8803
                     /gene="timP"
                     /locus_tag="ACHOUK_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFP9896071.1"
                     /translation="MKIRCFCIVLIVSGALLTEVNNNRSLSGDNLLVINNLQSSR"
     gene            complement(8835..9611)
                     /gene="sseB"
                     /locus_tag="ACHOUK_000560"
     CDS             complement(8835..9611)
                     /gene="sseB"
                     /locus_tag="ACHOUK_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFP9896072.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQVAGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(9754..11037)
                     /gene="pepB"
                     /locus_tag="ACHOUK_000561"
     CDS             complement(9754..11037)
                     /gene="pepB"
                     /locus_tag="ACHOUK_000561"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFP9896073.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVASDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(11215..11415)
                     /gene="iscX"
                     /locus_tag="ACHOUK_000562"
     CDS             complement(11215..11415)
                     /gene="iscX"
                     /locus_tag="ACHOUK_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFP9896074.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(11427..11762)
                     /gene="fdx"
                     /locus_tag="ACHOUK_000563"
     CDS             complement(11427..11762)
                     /gene="fdx"
                     /locus_tag="ACHOUK_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFP9896075.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(11764..13614)
                     /gene="hscA"
                     /locus_tag="ACHOUK_000564"
     CDS             complement(11764..13614)
                     /gene="hscA"
                     /locus_tag="ACHOUK_000564"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFP9896076.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(13631..14146)
                     /gene="hscB"
                     /locus_tag="ACHOUK_000565"
     CDS             complement(13631..14146)
                     /gene="hscB"
                     /locus_tag="ACHOUK_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFP9896077.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(14242..14565)
                     /gene="iscA"
                     /locus_tag="ACHOUK_000566"
     CDS             complement(14242..14565)
                     /gene="iscA"
                     /locus_tag="ACHOUK_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFP9896078.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(14582..14968)
                     /gene="iscU"
                     /locus_tag="ACHOUK_000567"
     CDS             complement(14582..14968)
                     /gene="iscU"
                     /locus_tag="ACHOUK_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFP9896079.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(14996..16210)
                     /gene="iscS"
                     /locus_tag="ACHOUK_000568"
     CDS             complement(14996..16210)
                     /gene="iscS"
                     /locus_tag="ACHOUK_000568"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFP9896080.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(16322..16810)
                     /gene="iscR"
                     /locus_tag="ACHOUK_000569"
     CDS             complement(16322..16810)
                     /gene="iscR"
                     /locus_tag="ACHOUK_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFP9896081.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(17111..17848)
                     /gene="trmJ"
                     /locus_tag="ACHOUK_000570"
     CDS             complement(17111..17848)
                     /gene="trmJ"
                     /locus_tag="ACHOUK_000570"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFP9896082.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            17967..18770
                     /gene="suhB"
                     /locus_tag="ACHOUK_000571"
     CDS             17967..18770
                     /gene="suhB"
                     /locus_tag="ACHOUK_000571"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFP9896083.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            18915..19769
                     /gene="yfhR"
                     /locus_tag="ACHOUK_000572"
     CDS             18915..19769
                     /gene="yfhR"
                     /locus_tag="ACHOUK_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9896084.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVNFI
                     LSALNPQN"
     gene            19960..21240
                     /gene="csiE"
                     /locus_tag="ACHOUK_000573"
     CDS             19960..21240
                     /gene="csiE"
                     /locus_tag="ACHOUK_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFP9896085.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(21232..22371)
                     /gene="hcaT"
                     /locus_tag="ACHOUK_000574"
     CDS             complement(21232..22371)
                     /gene="hcaT"
                     /locus_tag="ACHOUK_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFP9896086.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(22531..23421)
                     /gene="hcaR"
                     /locus_tag="ACHOUK_000575"
     CDS             complement(22531..23421)
                     /gene="hcaR"
                     /locus_tag="ACHOUK_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFP9896087.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            23557..24918
                     /gene="hcaE"
                     /locus_tag="ACHOUK_000576"
     CDS             23557..24918
                     /gene="hcaE"
                     /locus_tag="ACHOUK_000576"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFP9896088.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            24915..25433
                     /gene="hcaF"
                     /locus_tag="ACHOUK_000577"
     CDS             24915..25433
                     /gene="hcaF"
                     /locus_tag="ACHOUK_000577"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFP9896089.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEICYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            25433..25753
                     /gene="hcaC"
                     /locus_tag="ACHOUK_000578"
     CDS             25433..25753
                     /gene="hcaC"
                     /locus_tag="ACHOUK_000578"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFP9896090.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            25750..26562
                     /gene="hcaB"
                     /locus_tag="ACHOUK_000579"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             25750..26562
                     /gene="hcaB"
                     /locus_tag="ACHOUK_000579"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFP9896091.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIAGEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            26572..27774
                     /gene="hcaD"
                     /locus_tag="ACHOUK_000580"
     CDS             26572..27774
                     /gene="hcaD"
                     /locus_tag="ACHOUK_000580"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFP9896092.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDEQHLPYER
                     PPLSKSMLLEDSPQLQSVLPAHWWQENNVHLHSGVTIKTLGRDTRELVLANGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANDQLAREANLDTTNGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNHAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRSIRKWIQSGKTFDAKQLTD
                     ENIALKSL"
     gene            27871..28293
                     /gene="yphA"
                     /locus_tag="ACHOUK_000581"
     CDS             27871..28293
                     /gene="yphA"
                     /locus_tag="ACHOUK_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFP9896093.1"
                     /translation="MNSLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(28341..29213)
                     /gene="yphB"
                     /locus_tag="ACHOUK_000582"
     CDS             complement(28341..29213)
                     /gene="yphB"
                     /locus_tag="ACHOUK_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFP9896094.1"
                     /translation="MTIYTLSHGSLKLGVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIIETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(29225..30319)
                     /gene="yphC"
                     /locus_tag="ACHOUK_000583"
                     /pseudo
     CDS             complement(29225..30319)
                     /gene="yphC"
                     /locus_tag="ACHOUK_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
     gene            complement(30352..31350)
                     /gene="yphD"
                     /locus_tag="ACHOUK_000584"
     CDS             complement(30352..31350)
                     /gene="yphD"
                     /locus_tag="ACHOUK_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9896095.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSHHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(31375..32886)
                     /gene="yphE"
                     /locus_tag="ACHOUK_000585"
     CDS             complement(31375..32886)
                     /gene="yphE"
                     /locus_tag="ACHOUK_000585"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFP9896096.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGEIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAHKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVIRAVKKMSALGVAVIYVSHRMEEIRRIASS
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHAPVNVDELMSAILSVH"
     gene            complement(32909..33892)
                     /gene="yphF"
                     /locus_tag="ACHOUK_000586"
     CDS             complement(32909..33892)
                     /gene="yphF"
                     /locus_tag="ACHOUK_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFP9896097.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(33989..37270)
                     /gene="yphG"
                     /locus_tag="ACHOUK_000587"
     CDS             complement(33989..37270)
                     /gene="yphG"
                     /locus_tag="ACHOUK_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HFP9896098.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTRLDAYEE
                     KRFEQFFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCD
                     ALLDDAVALTPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLSAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPINQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWCLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNEAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGTTPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICAKAQGDETEATRCLHLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQHTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLAAAGLGEIAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            37388..38581
                     /gene="yphH"
                     /locus_tag="ACHOUK_000588"
     CDS             37388..38581
                     /gene="yphH"
                     /locus_tag="ACHOUK_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP9896099.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGEHWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVET
                     QLRQI"
     gene            complement(38644..39897)
                     /gene="glyA"
                     /locus_tag="ACHOUK_000589"
     CDS             complement(38644..39897)
                     /gene="glyA"
                     /locus_tag="ACHOUK_000589"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFP9896100.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEVEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            40225..41415
                     /gene="hmpA"
                     /locus_tag="ACHOUK_000590"
     CDS             40225..41415
                     /gene="hmpA"
                     /locus_tag="ACHOUK_000590"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFP9896101.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(41460..41798)
                     /gene="glnB"
                     /locus_tag="ACHOUK_000591"
     CDS             complement(41460..41798)
                     /gene="glnB"
                     /locus_tag="ACHOUK_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFP9896102.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(41859..43193)
                     /gene="glrR"
                     /locus_tag="ACHOUK_000592"
     CDS             complement(41859..43193)
                     /gene="glrR"
                     /locus_tag="ACHOUK_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFP9896103.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(43183..43896)
                     /gene="qseG"
                     /locus_tag="ACHOUK_000593"
     CDS             complement(43183..43896)
                     /gene="qseG"
                     /locus_tag="ACHOUK_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFP9896104.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(44061..45488)
                     /gene="qseE"
                     /locus_tag="ACHOUK_000594"
     CDS             complement(44061..45488)
                     /gene="qseE"
                     /locus_tag="ACHOUK_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFP9896105.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELDNVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(46064..49951)
                     /gene="purL"
                     /locus_tag="ACHOUK_000595"
                     /gene_synonym="purI"
     CDS             complement(46064..49951)
                     /gene="purL"
                     /locus_tag="ACHOUK_000595"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFP9896106.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIATLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            50208..51764
                     /gene="mltF"
                     /locus_tag="ACHOUK_000596"
     CDS             50208..51764
                     /gene="mltF"
                     /locus_tag="ACHOUK_000596"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFP9896107.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHMQ
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(51761..52264)
                     /gene="tadA"
                     /locus_tag="ACHOUK_000597"
     CDS             complement(51761..52264)
                     /gene="tadA"
                     /locus_tag="ACHOUK_000597"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFP9896108.1"
                     /translation="MSEVEFSHEYWMRHAMTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(52322..52957)
                     /gene="yfhb"
                     /locus_tag="ACHOUK_000598"
     CDS             complement(52322..52957)
                     /gene="yfhb"
                     /locus_tag="ACHOUK_000598"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFP9896109.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQALQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            53166..54014
                     /gene="yfhH"
                     /locus_tag="ACHOUK_000599"
     CDS             53166..54014
                     /gene="yfhH"
                     /locus_tag="ACHOUK_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9896110.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            54070..54330
                     /gene="yfhL"
                     /locus_tag="ACHOUK_000600"
     CDS             54070..54330
                     /gene="yfhL"
                     /locus_tag="ACHOUK_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFP9896111.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPQHVETEEQLWDKFVLMHHADKI"
     gene            complement(54524..54604)
                     /gene="shoB"
                     /locus_tag="ACHOUK_000601"
     CDS             complement(54524..54604)
                     /gene="shoB"
                     /locus_tag="ACHOUK_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFP9896112.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(55026..55406)
                     /gene="acpS"
                     /locus_tag="ACHOUK_000602"
     CDS             complement(55026..55406)
                     /gene="acpS"
                     /locus_tag="ACHOUK_000602"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFP9896113.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWEIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVANMHVTLADERHYACATVIIES"
     gene            complement(55406..56137)
                     /gene="pdxJ"
                     /locus_tag="ACHOUK_000603"
     CDS             complement(55406..56137)
                     /gene="pdxJ"
                     /locus_tag="ACHOUK_000603"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFP9896114.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(56149..56877)
                     /gene="recO"
                     /locus_tag="ACHOUK_000604"
     CDS             complement(56149..56877)
                     /gene="recO"
                     /locus_tag="ACHOUK_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFP9896115.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGVTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(56889..57794)
                     /gene="era"
                     /locus_tag="ACHOUK_000605"
     CDS             complement(56889..57794)
                     /gene="era"
                     /locus_tag="ACHOUK_000605"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFP9896116.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVATIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(57791..58471)
                     /gene="rnc"
                     /locus_tag="ACHOUK_000606"
     CDS             complement(57791..58471)
                     /gene="rnc"
                     /locus_tag="ACHOUK_000606"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFP9896117.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(58744..59718)
                     /gene="lepB"
                     /locus_tag="ACHOUK_000607"
     CDS             complement(58744..59718)
                     /gene="lepB"
                     /locus_tag="ACHOUK_000607"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFP9896118.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVDKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(59734..61533)
                     /gene="lepA"
                     /locus_tag="ACHOUK_000608"
     CDS             complement(59734..61533)
                     /gene="lepA"
                     /locus_tag="ACHOUK_000608"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFP9896119.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(61731..62210)
                     /gene="rseC"
                     /locus_tag="ACHOUK_000609"
     CDS             complement(61731..62210)
                     /gene="rseC"
                     /locus_tag="ACHOUK_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFP9896120.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(62207..63163)
                     /gene="rseB"
                     /locus_tag="ACHOUK_000610"
     CDS             complement(62207..63163)
                     /gene="rseB"
                     /locus_tag="ACHOUK_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFP9896121.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(63163..63801)
                     /gene="rseA"
                     /locus_tag="ACHOUK_000611"
     CDS             complement(63163..63801)
                     /gene="rseA"
                     /locus_tag="ACHOUK_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFP9896122.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHEWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(63834..64409)
                     /gene="rpoE"
                     /locus_tag="ACHOUK_000612"
     CDS             complement(63834..64409)
                     /gene="rpoE"
                     /locus_tag="ACHOUK_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFP9896123.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(64406..64561)
                     /gene="rseD"
                     /locus_tag="ACHOUK_000613"
     CDS             complement(64406..64561)
                     /gene="rseD"
                     /locus_tag="ACHOUK_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFP9896124.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            64817..66439
                     /gene="nadB"
                     /locus_tag="ACHOUK_000614"
     CDS             64817..66439
                     /gene="nadB"
                     /locus_tag="ACHOUK_000614"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFP9896125.1"
                     /translation="MNTLLEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGEESYHLTREGGHSHRRILHAADATGREVETTLVSKALNHPNIRVLERS
                     NAVDLIISDKIGLPGTRRVVGAWVWNRNKEKVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDVRGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHDISTLPPWDESRVENPDERVIIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(66424..67161)
                     /gene="trmN"
                     /locus_tag="ACHOUK_000615"
     CDS             complement(66424..67161)
                     /gene="trmN"
                     /locus_tag="ACHOUK_000615"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFP9896126.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAATQAQENVAQSPWLERINVHT
                     ADIQQWVTQQTARFDLIISNPPYYEQGVECATPQREQARYTTSLDHQTLLTCAAECIT
                     EEGFFCVVLPEQIGNSFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            67293..68627
                     /gene="srmB"
                     /locus_tag="ACHOUK_000616"
     CDS             67293..68627
                     /gene="srmB"
                     /locus_tag="ACHOUK_000616"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFP9896127.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(68660..69541)
                     /gene="yfiE"
                     /locus_tag="ACHOUK_000617"
     CDS             complement(68660..69541)
                     /gene="yfiE"
                     /locus_tag="ACHOUK_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9896128.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEHSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEDCFLPG"
     gene            69644..70231
                     /gene="eamB"
                     /locus_tag="ACHOUK_000618"
     CDS             69644..70231
                     /gene="eamB"
                     /locus_tag="ACHOUK_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFP9896129.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATTHGFHQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQT
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWIVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLIYCAVRIFY"
     gene            complement(70287..70670)
                     /gene="grcA"
                     /locus_tag="ACHOUK_000619"
     CDS             complement(70287..70670)
                     /gene="grcA"
                     /locus_tag="ACHOUK_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFP9896130.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            70975..71664
                     /gene="ung"
                     /locus_tag="ACHOUK_000620"
     CDS             70975..71664
                     /gene="ung"
                     /locus_tag="ACHOUK_000620"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFP9896131.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQHGETPI
                     DWMPVLPAESE"
     gene            complement(71712..72749)
                     /gene="yfiF"
                     /locus_tag="ACHOUK_000621"
     CDS             complement(71712..72749)
                     /gene="yfiF"
                     /locus_tag="ACHOUK_000621"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFP9896132.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            72956..73375
                     /gene="trxC"
                     /locus_tag="ACHOUK_000622"
     CDS             72956..73375
                     /gene="trxC"
                     /locus_tag="ACHOUK_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFP9896133.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            73444..74142
                     /gene="tapT"
                     /locus_tag="ACHOUK_000623"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             73444..74142
                     /gene="tapT"
                     /locus_tag="ACHOUK_000623"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFP9896134.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            74174..76834
                     /gene="pat"
                     /locus_tag="ACHOUK_000624"
     CDS             74174..76834
                     /gene="pat"
                     /locus_tag="ACHOUK_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFP9896135.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATEIAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAKAHLLLQQAIAEGATSLDTHEVQPILQSYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMTWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            76948..78303
                     /gene="pssA"
                     /locus_tag="ACHOUK_000625"
     CDS             76948..78303
                     /gene="pssA"
                     /locus_tag="ACHOUK_000625"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFP9896136.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            78349..78672
                     /gene="yfiM"
                     /locus_tag="ACHOUK_000626"
     CDS             78349..78672
                     /gene="yfiM"
                     /locus_tag="ACHOUK_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFP9896137.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKEFWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(78669..79967)
                     /gene="kgtP"
                     /locus_tag="ACHOUK_000627"
     CDS             complement(78669..79967)
                     /gene="kgtP"
                     /locus_tag="ACHOUK_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFP9896138.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDSALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(80084..>80276)
                     /locus_tag="ACHOUK_000628"
     CDS             complement(80084..>80276)
                     /locus_tag="ACHOUK_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9896139.1"
                     /translation="KAIRQDGLFALVKKMPDATLRVLYSHICQIQQRIRFPQLAYFHT
                     CPPYQKFILKLLNNHFPAN"
BASE COUNT        19090 a        21874 c        20222 g        19090 t
ORIGIN      
        1 tgtatatttc actgaaatga aaaatgtact gacgcgattt gccctacgtc atccatcacc
       61 agcaattgat aatcaccttt atccgtcaaa tgcagggtca cgttgcgccc gcgttcagtt
      121 aacggttcgc cattcagaaa ccaccagcgt tcacctgccc ctccacttga ttgcaacggc
      181 aaagtcgctt ccgctgcgcc cggtaaacgt ttaataatct cgccatcgcg aacgcctgtc
      241 agctgcagcg ggagctgggc atcgtgaccg tatggcgggc aaattgtcga ggccggtggt
      301 aagcgcacag cgcgacgttc tgatgcgggc aaccagggtt ccagcggtag cggccagaca
      361 tttatcattt cttgtcgcgc ttgcgggcaa tcagcggcaa cacgtttgcc attttcatcc
      421 agccagatgg ggaaacgaat gccattaatg ccttcctgct ccggcagtaa tagagttggc
      481 ggctgactcc cgtccagcag ccaggttgcc aggcggcggc gacagttacc gtcaccttcc
      541 ggcaaagact gtccgcccgg ccagcaaata acgccacgag tgactgagtt cgggcgcggg
      601 tcttccggca gattagcact gcgcgacagt aagatattat tgacctgatt caacaatggt
      661 acggcgctgg caaaaccaaa ctgaccaaca acgggcgtgc cgtccggtct gccagtccag
      721 atcccaatga catagcgagc gttaacccca atcgcccagg cgtcacgata gccatagctg
      781 gtgcccgttt tccatgccag tggggcgacg cgcggcaagg caccatccgg caagggtagc
      841 gcttcatcag ccataatccg gcgaatgatc cacgccgctc ccgacgacat taaaggccgt
      901 tcaagcagcg gatcgtcagg ctgtaagcgc aatttgcctg ccttgccgtg gcgagcaaac
      961 gcggtatacg ctgccgccat atcttccagt ttcgcaccag cgccgccgag aatgagcgaa
     1021 agattcggcg cagcaccgtt gggcaaatat aacggcaatc caacattgcg taactttgcc
     1081 gcaaaccgtt tcggtccata ggcttccagc acctgcacag caggtaaatt cagcgagcgc
     1141 accagcgcct cgctcatgct gatcgggcca tgaaaaccgc tgtcaaagtt gccaggtcgg
     1201 taatcaccgg tacgccgggg gacgtcttgc agcagtgatg ccgggtggat caatccttca
     1261 tccagcgcca gaccgtaaac aaacggtttg agcaccgatc ctggcgatcg gatcgcattg
     1321 accatatcaa catgaccaaa gcgtgaatca tcgttgagat caaccgaacc cacccagccg
     1381 cgaacacgca tatcggtatg atcaaccacg atcatcgcca gtgagctgcg cggtggtaat
     1441 cgtcctttcc agttttgcgc cagttcttcc agacgtcgtt gaagaccggc atccagcgta
     1501 gtaacgattt tgtcgctttt gcttttaccg agcatcatgc gcgaaaacag cggtgccagt
     1561 tgcggcattt gtcggggtgc cagccagatg ggttcttccc ttgactcttt tacctgctca
     1621 cgggaccaca caccttgcac ggccatccgt tcgagcactt tattacgcgc gacttcggca
     1681 cgctccggcc aacgatccgg gcgaagacgg ctgggcgctt gcggcaaaac cgccagcatt
     1741 gccgcctcgg aatagcttaa attcgcaggc gactttccga gataagccca acttgccgca
     1801 ccgatcccct gcaacgtacc gccaaacgga gcgcggttaa gatacaaggt cagaatttca
     1861 cgcttagaca gatgccattc cagttgcaac gcgcgccaga gctggcgaat tttgccgcca
     1921 aatgttttgg ggtgaggatc aagcagacga gcaacctgca tagtgagcgt gctgccaccg
     1981 gaaataaccc gtcccgaagt gagatcttgc catgctgcgc gcgccaccga gaatggattc
     2041 accccaggat gcttccagaa ccagcgatct tcataattga tcagcgcttc aaggtaacgt
     2101 ggagaaacat cttcgattgt tacaggataa cgccagatgc cgtcagcatc ggcgaagcgc
     2161 cagagcggcg taccatcctg cgccacgacc actcgtgcgg gattgacttc atgcagcggt
     2221 agaggccaga gtttatctgc tccccacgct gcaagaaaaa ggagaaatgg cgcagctgcc
     2281 agcgttatcc agcagccgcg tttggttaac aagcgaggca tttacggtct gacaatcagc
     2341 aaatcttcag ccgcgccggt cgcccgccat tggggaacat acattgattc caccatcggt
     2401 tgcggtacct gatacgttcc cggcgtcacc gcccgcgcca gatacaccag cgttaccggt
     2461 tggtattcat caacggcaac cgccgccaca aagcgatcgt cacggaactc aatgtgctta
     2521 atactcgcct gctgcatctg gttcagtaag ttctgcactt cgccaccact ttgctccagg
     2581 ctggcgctac cgttcgccag attctggttt tccagttcca gacccgcagg cagcagatcc
     2641 acgactaacg catccggcac gctgttacta gcttttacct gcaaccagac cagcaccaga
     2701 tcgccgctac gtaacgagtc cagcgatttg ctcttaccat cagtaccaag aatatgacgc
     2761 tcaatttgca gcacattgtt cgccggtaaa ggtgcggatt gcggataacc gctggcatcc
     2821 acacgcagcc ataacggctg ctcgccactg ttggtcacct gcaaggtggc aagttgatcg
     2881 ctattcagat tgctgttttg cgctttctcg cctgtcagcg gctcagctga gaaagaggtt
     2941 tgcgcctgcc atttacctgg taaatcctga atcgtacggg cagccaggaa caacgcgtta
     3001 ctttcctgcg tcgatagcca gcgttcacca aacgcctgct gcgaaagtgt gttcagcagg
     3061 gagtactgct catcgggtag cagtttgttc tcttccagca aggagagcat caacgcgttg
     3121 tcgcgcagtg gactaccgta atcacccagc catatccgct catcactatt acgcggcgtt
     3181 tttagcgcca gcgcaatcgc ttcttcatca cgcgtcgcat cacccatggt tttcagcgca
     3241 acgccaagtt gcagcagcgg taaaccagaa gcggcatctg cacgatgctc ccagatttca
     3301 cgcagcgcac ccagcggagc cttttgctga cgggccaaca ccagcgcagc gtaagactgt
     3361 acggcgaatt tactggcttt gagattatcc gcgtacggga tcgacatcat gcccggatct
     3421 tgtaaatagc gcagcagacg ctcattaccc cggttaatgg cgtctgtcgg cacgctgtaa
     3481 ccctgttcgc ctgcgcggac caggaaatcc atcacgtaag ccgtcagcca gtactcttcg
     3541 tcaccgtttt tatcccacag cgcaaagccg ccgttatcac gttgcatttg cagcaaacgg
     3601 gaaatgccga tatcgaccga tgcacggcgt ttctcatcac tgtcgccttt gatgcccaac
     3661 gcctgcagtt gggctgcgtt ggtataaagt gacgggaaca ggccgctggc ggtttgctca
     3721 agacagccgt acggatacgc ttttaactct ttgatataac gtgcgatgtt cagcggtggt
     3781 ttgccgctca acaacaattg cccttccagc gtaacaggcg agaagttttg caatccatcc
     3841 gccggaatcg cccatgtctc accaggctgt aacgccgtac cgtaattaac cgtttgtgcc
     3901 gggaacgccg gacggacgcc gattttccac tgcttatgct gatcggcaac ggtttcaccc
     3961 ggtaacgcta acccgctaat ggtggcctga atttctccat cgccataacc cggcaatgct
     4021 cgcaccggga taaacagcgt agtacgcaca cctggcgcta attcaacggc tgcgggtgaa
     4081 tcgctgacca gttcaagcaa accactggcg gtcagggcaa cgttcagttt ttgcggttta
     4141 tcggtaagat tagtgatatc cagcgtcaga cgcgagttat cgccactcgc cataaagcgc
     4201 ggcatgttca gttcagcaat caccggtgcg gcaactatca ctttgctttc gttgctaccg
     4261 aattcatctg ccgtccaggc ttgcgccatg acgcgcaatt caccgttaaa atcgccaatc
     4321 ggcagtgtaa ccgagccttc gccctgttcg ttgagcgtta ccggcagcgc ctgctgcgcg
     4381 acaatattga cgtgattgac cggcggttta ccaccacgtt tcagctcatc accatcgcca
     4441 ccgaaacgca gagctgccag acgcccctga ccttcaataa cctgaccgta aatatcgtaa
     4501 atgtctgcgc catagcgttt ctgaccaaag aacgcctgcc acggatctgg cgtgacgtag
     4561 tcagtaatat tcagcacacc actatcaacg gcagacacca gcacattcac ctgtttaggc
     4621 ttctcgccat ttttagtgct ggctttaatt ttcacggtta atggttgatt gggacgcatt
     4681 tttgctggtg tttccagcgc cagatcgagg cgacggtttt catcaccaag cggcagatgc
     4741 aacacaccaa ccgcgcgttt tggcgtcgcg gagcgagatt tatcgccagg acgcaccacc
     4801 agcgtactca aataaagatc atgacgattc caggttttat cgaccggaat cgtcagatcc
     4861 agcccttgag ccggaacatc aatctcttgc caccacagcg gcccttcact ggactcgacc
     4921 atcgcataac ctttacccgc cgttggcgcg gcgatatgca acttaatggt atcgccaggg
     4981 cgataaccgg ctttatccag tttcagcgtg acacggtcgg gtcgcactgc gccgctaccg
     5041 tcgctattat cctgccagct atagccagcc caaaaacgaa cactactgac cgcttcattc
     5101 ggcgctttga cttccagacg ataagcgccc cactctaccg ggaaactgac cttgccggtt
     5161 tcgtccgctt tcagatccag agtttgttca ttttcaatca gatctttttg atcaaactgt
     5221 gattgccagc cttcatcttc tgaccagttc cagtagtaat cgcggcgttc gcgaatcagg
     5281 cgcacctgca agcccgacac ggcttttttc acgccttgcg catcgctata aacgatgtca
     5341 aaagcggcgt tactgccttc atcaacaatg ggctgtttca ccgtgctgtc agtgcgataa
     5401 tcgtaaaccg atttcgaggc gaactgcgga cggatccccg gcaatgcatc ggcaggccag
     5461 atagcctgct cagcgcggcg cgtcaccggg cgaccgcccg attccagcaa actgccctgg
     5521 aaaataacct gcaatgggga atgcgtttcc ttccactggc tttctgtaga aacttcgccg
     5581 cgccctttat catccagcgt caactgaact tcatccagcg tgcgggaaag gttttcggca
     5641 gctatatcgc cgaattcaaa accaggtaag gctgacacag cctcacgcag tgggcgcagg
     5701 aaaagttgcc cttgcaaagt attaccatta gcaggcgcac cgtacaggta gtatcccacc
     5761 acggagaatt tcacttcatc attcggcgta agcggggttt tctcaccggt cagattcagc
     5821 gccatacgct ctggcataaa atcttcgacg tggaaatccc acatccgata ctggttatcg
     5881 cccgtgttag cgcgaatatg ccacataccg gttgccgcat tgctatcgag tggccaggta
     5941 aagtggtaga ggccattttc cggctgacta acgacgctcc tgagtacctg cccatcgggt
     6001 ttaatcacgt ctaacttgat gggttgattg ggcaacgctt taccgtctgc atcacgcagc
     6061 aaaccattga ggattaccgt ttcacccggg cgataaagat cgcgcgggcc aaacatgaaa
     6121 aactgtttgc tatagcctgg cgcgccagca atgttaaatt ctgctaagtc cagcgccgga
     6181 agttttaaat cgagtagcgt tgtctgaccg tctttacgtg ccagcaataa tgcggcgttt
     6241 ttatcatttt ccagctgcac atgcccctga gcgtcactgg ttgcctgagt cagagtctgc
     6301 cctttctcat ttaataaaga gacttcaatt ccttgctggg ccgcgccgtt ttccagactt
     6361 tgggtaaaga tatccagacg attgtgataa cggtgagctg aaacgccgat atcacttaac
     6421 gtaaacagcg tcgcgggatt actgtaatcg taacgtccag cctgattcat cacagccaga
     6481 tacacgcccg cctgctgaag cggtttgata tcgcccagcg gcagcaataa tttttcacgg
     6541 gtgttacgcg caggattgag atcaaaccgt ccggtgtaga ccagatccgc catctgcagc
     6601 agtttgtctg actgccagtt cgccagcgaa ttgcggtatt cccattggct aatgaatgct
     6661 ggcagagatt ctggcttaac gcggaagaag ttaacatcga cattattaac gttgagcgcc
     6721 attaccggca gcccttcaac gactttacca ggcagcagcg aaccacggct ggcaaaaccg
     6781 acactgggtt ggatgtcgcg ggtagttata gttttttcgt aatctttact gaaggttgcg
     6841 ttgttgagtg ctttgacctc cttgccaatt gtaacgatca aatcacgttt cggttcgagg
     6901 tggcgtaaac gcagctcttt aagattatct gatagctccc aggcaccatc cactttgccg
     6961 ctttttttat cgacgacatg aataacgcgt gagaaatcct gatccgggtc gagagggatg
     7021 gagaacgtca gcaccagcgt ggctgcacca tcaagttgga cttcagagag atccagcaat
     7081 gtcagcgcct taccggcact ctgttgggcc agtttttgtc gctctggcgc ggagagtttt
     7141 gctgaactcg cgttttctga agaggccgct ttagtaactt cagaaggcgc atcttttttc
     7201 accgctgttg gcgcgttatc gttgttgtcg caccctgcca gcgccagcat tagcatgcag
     7261 gcggctacgc gtaacttttt catttttcat ccctgccaca atggcccgtt agcaacgtcg
     7321 aataattatt atgcgtgaga atttacgtta tgtaattcag tatcacgccc atacaatccg
     7381 aattttagtg agaattgtgg ctcagacagc caaatcagcc tcttttgctt gtcgcttttc
     7441 actaaacatc cgacaattta gcctaacccc ggcaaaaatg gagatgccta tgtccacgac
     7501 atggtttgta ggagccgact ggctcgccga acatattgat gacccggaaa ttcagattat
     7561 cgatgcccgc atggcgtcgc ctggacagga agatcgtaac gttgctcagg agtatctgaa
     7621 tggacatatt cccggcgcag tgttttttga tatcgaagcg ctttctgatc acacttcccc
     7681 gcttccgcac atgctgccgc gcccggaaac gttcgccgtg gcgatgcgtg aattaggcgt
     7741 taaccaggat aagcacctga ttgtctatga cgaaggtaat cttttctcag ccccacgagc
     7801 atggtggatg ctgcgcacct ttggtgtaga gaaagtgtcg attctggggg gtggacttgc
     7861 aggctggcag cgcgatgatc tgttgttaga agaaggtgca gtagagctgc cggaaggaga
     7921 gtttaacgcc gcgtttaatc ctgaagcagt ggtgaaagta accgatgtgt tattggcaag
     7981 ccatgaaaat acggcgcaaa ttattgatgc ccgcccggct gcacgtttta acgcagaagt
     8041 tgatgaacct cgcccaggtt tacgtcgcgg acatattccc ggtgcattga acgttccgtg
     8101 gacggaactg gtgcgcgaag gcgaactaaa aacgaccgat gaactggatg cgatattttt
     8161 tggtcgcggc gtcagctatg acaaaccaat tatcgtcagc tgcggctctg gtgtaacagc
     8221 agccgtggtt ttgttggcac tcgcgacgct ggatgtgcca aacgtgaaac tgtacgacgg
     8281 cgcatggagt gaatggggcg cgcgggcaga tttaccggtt gagccattga aataagcttt
     8341 ttacaggcaa taaaaaaccg ccgaatttgg cggtttttta ttgctggtct ggttcgcggc
     8401 ctttccagca ggttgacttg tgttgccgga gcaacgcagg caccccacag tgttgaaata
     8461 ctctctttta tgcctggctg tgtcaagata ataaacagag taattattca aacatgagag
     8521 tattattctt aatggccctt ttcgccgtct cgcaaacggg cgctggcttt aggaaagaat
     8581 gttccatgac cgtaaataca ggcaccccac agtgcttgct atcgcggcaa tatcgccagt
     8641 ggtgctgtcg tgatgcggtc ttcgcatgga ccgcacaatg aagatacggt gcttctgtat
     8701 cgtacttatt gtttctggtg cgctgttaac cgaggtaaat aataaccgga gtctctccgg
     8761 cgacaattta ctggtgatta acaaccttca gagcagcagg taagcccgaa tgccgccctt
     8821 tggggcggca tattttagat tatccgattc tgtttaaagt cacgcaaaaa accaccccag
     8881 cgacgttcat agaatggcgc aatatgttcg gtaataaagt ggctaattcc tttttcccct
     8941 tttttcacct gacaaatatc gattggttca tcgccaggta atgtatcggt cgctacactt
     9001 cccgccacct gaataatttc ttcgatatca ccatcggctt caatgccaat aagtaaatta
     9061 ggctgtgcct cttcgttctc tttaattgaa caaataaaag cacgcttcac aggcttaatg
     9121 gttttaaata aggtggtgag tgaatcaatc atttgtgctg gcggctctgc gacttccgat
     9181 aatatcagcg attcaccgcc ttccaggatt tcctggctgc tcagcggatt tccctcttca
     9241 ccaatcaaca aactgatttc acgcggcata aattctttac cggtcggcag tttggcatta
     9301 aggaagagcg tttcgccaag ggtcatctca aacaatgtgc gaacgggcat tacgacaaat
     9361 gcctgttcgt cttcaaccgc ctgttgaagt gcttctaacg aggtaaaaaa aggaatgacg
     9421 ctggtgccgt cttctttttc ccagtgctgt aaatcaagcg cactatcttc aaccacagcc
     9481 tcgccctgcg ccgccgtacc aggcacccag acggtggatt ccagtagagt acggaaaaag
     9541 gccgggcggt gcgccggttc ggttgctgct ttttccagca ggtcttcaag ttcatttttt
     9601 gtttcggaca taagaaccac aattcattca acgttcgggg cacaagtgcc tgatgcgcca
     9661 tacttatcag gcctacaagg gattcacaat ttgttgaatt tgcaatactt tgtaggccgg
     9721 ataaggcgtt tacgccgcat ccggcatatt agtttacgcc gttaacagat tagcaatcgt
     9781 gcgcacacca agtcccgtag cacccgcaga ccactgttca accggcgctt tacggtaagt
     9841 cgccgagcag tcgatatgca gccagccttg ctgatagttc tcaacaaagt gcgacaggaa
     9901 gcccgctgcc gtgctcgcgc ctgccggata cgccgcgctt ccggtattgt tcagttcggc
     9961 aaagttagac ggcagctggc tgcggtggaa ttccgccagc ggcagacgcc agaatggttc
    10021 gttctcttgt gaggcactcg ccagcaaacg accggcaagc gcatcgtcaa aactgaacag
    10081 cgcgtgataa tcattaccca gcgcagtttt cgccgccccg gtgagggtcg ccgcatcaat
    10141 gatcatttcc ggtttctgcg cactggcatc aatcagacca tcggcaagca ccaggcgccc
    10201 ttcggcatca gtgttcatca cttcaacttt tttaccgttg cgataagtga tgatatcgcc
    10261 cagcttgaac gcattgccgc taatcaggtt atccgcacag cagaggaaca gcttcacgcg
    10321 cttgttcagt ccgcgcgtaa tggcaaatgc cagcgccccg gtaaccgttg ccgcgccgcc
    10381 catgtccgac ttcatcgagt ccataaacgc agtctgtttg atgctgtagc cgccggagtc
    10441 aaaagtgata cctttaccta ccaggcacgc gtacactggc gcttctttat cgccagttgg
    10501 gttgtaatcc agcgccagca ataccggaga acgttctgaa ccgcgtccga cggtgtgcag
    10561 ccccatataa ccttgctcac gcagatcttc gcctttggtg atccgataag tcacacgatc
    10621 gctcgcgacg ttgctgatca gatcaacagc acgctgtgcc agttgcgatg gtcccaattc
    10681 ttctgccggt gcgttgatgg tgtcacgcac ccagtcgatg atcatcaggc ggttatccag
    10741 ttcctggcgc tgggcatcgt ccagatccgg ccactccact ttacgcgtgc ctttcggggc
    10801 tttgtaacct tgccagaatg cccagcatcg atccgcatcc cagccttcac cgcttaactg
    10861 aacatgcttg atgcccagac cgtcaatctt gcgcgccgca cgctggatca gccccagatc
    10921 gtctgccccg ttcaaatgca gggtaatgcc gtcattatta atgctgtaag ttgctttttc
    10981 tccccagcgc gcgtcggcag gttgggtaga gagggtaatc ttcatcgctt ctgtcatttt
    11041 agttatcctt ctttgtaaaa gggccgccag ttggcagccc caggattttt ccgctaaatg
    11101 attcgcgttg caggaaggcg gcaagtgagt gaagccccag gagcatagat aactatgtga
    11161 ctggggtgaa cgagcgcagc caacgcatct gcggcgtgaa gcatgacgcg gaaattattc
    11221 ggcctcgtcc agccagacta acaaaatcgc ttcgaggatt ttctcgttgg atgcctgcgg
    11281 gtcgtcgtcg aaatcttcca gatcgcaaat ccactgatgc atatcggtga atcgaaccgt
    11341 tttcggatca agatcgggat acgcatcgta cagtgcttcg ccaatttcgc ggctatcggt
    11401 ccacttaagt cccatactaa cctctgttaa tgctcacgcg catggttgat cgtgtaacgc
    11461 gggatttcga ccactaaatc ttcgtcggtg acgcgcgcct ggcagcttaa acggctttcc
    11521 ggctccagtc cccaggcttt gtccagcatg tcgtcttcct gctctgagct ttctggaagc
    11581 gagtcaaaac cttcacgaac gatgcagtgg caggtggtgc aagcacagga tttttcacag
    11641 gcgtgttcaa tctcgatacc gttacgcaga gctgcgtcga gaatggtttc accgctatta
    11701 gcttccagaa cagcgccatc agggcagaga tcctgatgag gcaaaataac aatctttggc
    11761 atattaaacc tcgtccacgg aatggccttt cagcgcacga cgaaccgact ggtccatgcg
    11821 gcgagcggcg aaatcctggg tttgtttgtc tacgttttta atcgcttgtt cgatggcgtc
    11881 aacgtcatcg ccctgcgcca cttcactcag gtgagcggca gcatcgtcaa tgacctgacg
    11941 ttctgcggcg cttaacagcg cggcatcagc agccagcgcg ccgtgcagac tttccagcac
    12001 acgcgccgct tctacttttt gttctgccag cattcgggct tttacgtcct gctcggcata
    12061 gctcattgag tctttgatca tcgaagcgat ttcgctatcg gtcagaccgt aagacggttt
    12121 gacctgaata gacgcctcaa cgccggtgga tttctccatc gccgtcacgc tcaaaagacc
    12181 gtcggcatcg acctggaacg tcacgcgaat atgcgcaccg ccagccggta gcgccggaat
    12241 accacgcagc gcaaaacgcg ccagtgagcg gcagtcctgc accagttcgc gctcaccctg
    12301 cattacatgg atagacatcg ccgtctgacc atctttaaag gtggtgaaat cctgagcgcg
    12361 ggccactgga atagtggtat tacgcggaat cactttctcc accaggccgc ccatcgtttc
    12421 gaggcccagc gacagtggga tcacatcaag caacagcatt tcgctgtctg gcttgttacc
    12481 caccagaata tccgcctgaa tcgccgcgcc aatagcgacg actttatccg ggtcgatgga
    12541 agtcagcggt ggacgaccga aaaattcgcc tacccgttca cgcaccagcg gcacgcgagt
    12601 agaaccgccc accatcacca cttccagcac ttcatcagct tctacacccg cgtctttcag
    12661 cgcgcgacga caagccaata aggttcgttt taccagtggc gcgatcagtt cattgaattg
    12721 ttcacggctg atttcgccct gccagcccgc aacgttaacg gtcacggagt ccgcatcgct
    12781 cagcgcgatt ttggctgcaa tggcggcatc cagcagttca cgctgaacgc ggttatcgct
    12841 acgatcagga atgcccgcct gctcgcgaat gtaatccgcc agcagatggt cgaaatcatc
    12901 gccgccgagc gcggaatcac cgccggttgc cagcacttca aacacgccgc gacttaagcg
    12961 cagaatggaa atatcaaacg tcccgccacc gaggtcataa acggcgatca cgccttcctg
    13021 accggaatcc agcccgtagg cgatagccgc agcggtcggt tcgttaagta agcgcaggac
    13081 gtgaaggccc gccagacgcg ccgcgtcttt ggtgccctga cgctgggcat cgtcaaagta
    13141 cgccggaacg gtgataacta caccatccag ctcgcctgcc agggcttcag ttgcccgcgc
    13201 cgccagtgct ttgaggatgt ccgcagaaac gcgcaccggg ttcagcagcc ccgccgccgt
    13261 ttcaatcatc ggcaggccgt tttcgctggc ctggaattga taaggcagat gcggatagcg
    13321 ttgctggata tcagccagcg agcgtcccat caggcgttta acagaactaa ttgtgttggc
    13381 ggtatcgagc gctgcattag tacgcgcgtc ataacccacc gaatgccctt gctgttgata
    13441 gtgaacaaca gatggcagca ggtgacggcc ttcatggtcg gctaacgttt cggcctgacc
    13501 gctgcgcact gtcgccacca gcgagttggt tgtgcccagg tcaataccgg ccgccagacg
    13561 acgctgatgc ggcgcagcac tcaaaccagg ttcactaatt tgtaataagg ccatgtttag
    13621 cttccagaaa ttaaaaatcg agcagttttt cttcgagttg ttcggcactg cttcgcagtt
    13681 tatcgagaaa acgcagctta cgcacggtat ccgccgccgc gtcccaggtc tcgttgtcta
    13741 actgttcaac catcaactga tggcgggtat caaacatctt tttcacacgt ttgataaagc
    13801 tttccagccg cgcttcatct ttcgcctgtt cgatctcgtc cagctcttcg cgcagctcca
    13861 actgttccat caggaacgcg gtgtcgcgca cagtatgctg ctcgctggcg agatcaaagc
    13921 cgtgcaaaga aagcaaatat tccgcgcgca ttaacggatg acgcagcgtt tgccaggcct
    13981 gattaatggt tgcagattgc tgtacggcgg cgagttgttc cgcctggctt ccgctggcga
    14041 atttatcagg atgatactga cgttgtagat cctgaaaacg caggctcagc gcctgggtat
    14101 cgagttgata acgggcaggc aagccaaaga gggtgaagta atccataaca ttctcagggc
    14161 tgcgatccgc cgcgataacg gcgaatcagg taaaacaacc cccacgcgca ggcgaccacg
    14221 gtggggttat cggtatgcgc atcaaacgtg gaagctttcg ccgcaaccac actcatcttt
    14281 aacgttcggg ttggtgaatt taaacccttc gttcaggcct tcttttacga agtccagctg
    14341 cgtaccgtcc agaaattgca ggcttttgcc atcgaccacg actttcacgc ctttgtcttc
    14401 aaacacgatg tcttccggcg tcggttcgtc aacaaattcc agtacataag ccatacctga
    14461 acatccggag gttctcacgc ccagacgtag gccaaaccct ttgccacggt tagccagaaa
    14521 ggtatttact cgcgctgctg cactgtcgct aagtgtaatc gacataacca aacctcaact
    14581 cttattttgc ttcacgtttg cttttatagt ccgcaatggc ggctttgatc gcgtcttctg
    14641 ccagaataga acagtgaatt ttcaccggcg gcagttcaag ttcttcagca atatcggtgt
    14701 ttttgatagc ctgcgcttcg tcgagagact tccctttcac ccattcggtg accagggagc
    14761 tggaagcgat agcagaaccg cagccgtaag ttttaaaacg cgcgtcttca atgatacctt
    14821 catcgttgac tttaatctgc aacttcatta cgtcgccaca ggccggtgca cccaccatgc
    14881 cgctgccgac gttctcgtcg ttgttgtcaa aggaacccac gttacgcgga ttctcgtaat
    14941 ggtcgataac tttttcgctg taagccatta taaattctcc tgattccgat accgattaat
    15001 gatgagccca ttcgatgctg ttcagatcca cgccctgctt gtacatttcc cacagcggag
    15061 aaaggtcacg cagacgaccg atggatttac gaactaactc gatggtgtag tcgatctctt
    15121 cttcagtagt aaaacgacct aaagagaaac ggatagagct atgtgccagc tcgtcgttca
    15181 gccccagcgc gcgcagcacg taggacggtt cgaggcttgc tgacgtacag gcggaaccgg
    15241 aagaaaccgc gagatctttc agcgccataa tcagcgactc accttcaacg tagttgaagc
    15301 tgacgttgag aatgttcggc gcaccgtgtt ccaggtcacc gttcaggtaa acttcttcga
    15361 tatctttgat gccgttccac agacggttac gcaggccgcg cagacgttcc atctcggtcg
    15421 ccatctcttc ttttgcgatg cgataggctt cgcccattcc gacgatctgg tgaacaggca
    15481 gagtgccgga acgcataccg cgctcgtgac cgccgccgtg catttgcgct tcgatgcgta
    15541 cgcgcggttt acgacgcaca tacagcgcac cgataccttt cgggccatag attttgtgac
    15601 cggagaaaga catcaggtca actttcaact ggctcaggtc gataggcagt ttgcccacgc
    15661 tctgggttgc atcaacgtga tagataatgc cacgagcacg gcacatttcg ccgatagccg
    15721 cgatatcctg caccacgccg atttcgttat ttacgtgcat gatagaaaca aggatggtgt
    15781 cgtcacgcat cgctgcttcg agttctttca ggtcgataat accgttacgc tgcggtgcga
    15841 ggtaggtgac ttcgaaacct tcgcgctcca gctgacggca ggtatccaat accgctttgt
    15901 gttcggtttt gctggtgatt atgtgcttgc cttttttctg ataaaagttg gctgcacctt
    15961 tgatcgccag gttgtcagac tcggttgcac cagaggtaaa gacgatttca cgcggatcag
    16021 cgccgaccag atcggcaatc tgattacggg cgatatctac cgcttcttca gcctgccagc
    16081 cgaaacggtg agaacgggag gccgggttac caaaggttcc gtccatcgtc ataaactgca
    16141 tcattttctc ggcaacacgc gggtccaccg gcgtggttgc ggagtagtcg agataaatcg
    16201 gtaatttcat tgctctataa actccgtaca tcactcaatg caaggaacca ggctaccggc
    16261 tggatgtacg accgtgttta cggagcgcga tacgccccgg cctgattctg aatacttttt
    16321 attaagcgcg taacttaacg tcgatcgcgt cttgtgtgcg ggtgcgtggc gcgtcgtgag
    16381 tatgctgacg accagacaca tccagcactt cctggttgtt aaccagttcg cctaaagtaa
    16441 tgttattgag aaaaccggta agacggtcgc tcaaatcacg ccacagcgcg tgggtcaggc
    16501 atttatcgcc gccctggcag ccgcctttac cctgacaacg ggtggcatct acagattcgt
    16561 caacggcgct aatcacttcg ccaacggcga tgctgctggc atctttgcct aacagataac
    16621 caccgcctgg tccacgtacg ctggaaacca gaccattttt acgcagacga gaaaacagtt
    16681 gttccagata agaaagggaa attccctgac gttcggaaat atcagccaac ggtaccgggc
    16741 ccgcttcaga gttgagcgca acgtcaagca ttgcggtcac ggcatagcgc cctttagatg
    16801 tcagtctcat gtcttacttc acctcaaact cgcccctgcc cggggttttt tattgtaaaa
    16861 gtgggggtat tgcatagcag ggtcaagtct gacattcccg agtaaattgg tcaactattt
    16921 acttgactga tttagtcggg tatttaacct tcagtgccat ttttttatcg tggcgtttgt
    16981 acctgttatc cttccagacc atgggcgggt tgagggcaat tattcgcatt agcgcaggcc
    17041 tgttcccctc accctaaccc tctccccgaa ggggcgaggg gaccgtccgt gcacttatct
    17101 ggcttagccc tcaggcctta ttacctttat tctgctgctc tatagaagcc agaatcccgc
    17161 gcaggatatt caattcctgg ctttccgggc gcgcacgggt aaacagacgg cgcaatttat
    17221 tcatcacctg ccccggatgg ttttcacgga taaaaccggt tgccagcagc gtttgttcca
    17281 gatgaccata aaaacgctcc agatcatcga ccagcggata cggcgtctct tcatgctcga
    17341 cctgctcgcc attttcctga gttgccagcc aggccatacg cacttcatag gcaataacct
    17401 gaaccgccat cgccaggttc agcgagctgt attcagggtt agcggcaatc gcgacatgat
    17461 aatgacactt ctgcaactct tcattggtca ggccgacacg ttcgcgacca aacaccagcg
    17521 ccaccggggt atttgccgct tcagcaacgc ttttcagacc gcattcgcgc gggtcgagca
    17581 tcggccacgg cagcgtgcgg gaacgtgcgc tggtgcccac caccaggcta caaccggcta
    17641 acgcttcgtc gagcgtatcg acgatgtgag cattaccaat cacatcgctg gcccccgccg
    17701 ccagggcaat cgcctgggag tcaggtttca ccagtggatt aaccagccac agattggtta
    17761 atcccattgt tttcatggca cgggcaacag aacccatatt gccggtgtgt gacgtctcca
    17821 ccagcacaat tcgaatattt tgcagcattg tctttcttcg tctaaagatt attcgcgcat
    17881 cttatcataa aacgaagaca gatgccgatc tcgctgctat actctgcgcc gttttcccgt
    17941 tctttaacat ccagtgagag agaccgatgc atccgatgct gaacatcgcc gtgcgcgcag
    18001 cgcgcaaggc gggtaatttg attgccaaaa actatgaaac cccggacgct gtagaagcga
    18061 gccagaaagg cagtaacgac ttcgtgacca acgtagataa agctgccgaa gcggtgatta
    18121 tcgacacgat tcgtaaatct tacccacagc acaccatcat caccgaagaa agcggtgaac
    18181 ttgaaggtac tgatcaggat gttcaatggg ttatcgatcc actggatggc actaccaact
    18241 ttatcaaacg tctgccgcac ttcgcggtat ctatcgccgt tcgtatcaaa ggccgcaccg
    18301 aagttgctgt ggtatacgat cctatgcgta acgaactgtt caccgccact cgcggtcagg
    18361 gcgcacagct gaacggctac cgtctgcgcg gcagcaccgc tcgcgatctc gacggtacta
    18421 ttctggcgac cggcttcccg ttcaaagcaa aacagtacgc cactacctac atcaacatcg
    18481 tcggcaaact gttcaacgaa tgtgcagact tccgtcgtac cggttctgcg gcgctggatc
    18541 tggcttacgt cgctgcgggt cgtgttgacg gtttctttga aatcggtctg cgcccgtggg
    18601 acttcgctgc aggcgagctg cttgttcgtg aagcgggcgg catcgtcagc gacttcaccg
    18661 gtggtcataa ctatatgctg accggtaaca tcgttgctgg taacccgcgt gttgttaaag
    18721 ccatgctggc gaacatgcgt gacgagttaa gcgacgctct gaagcgttaa tgactcaggc
    18781 gggtgatatc actcacccgc cctcgccttt caggcgctat tccgaaatac ttcctcaccg
    18841 ctttactttc tttcgtcact ctcccaccat ctttccccga ttaatggatg ataaagaagt
    18901 cgcaaggaaa ggttatggca ctgccagtga acaaacgcgt tcccaaaatt ctgtttattc
    18961 tctttgttgt tgccttctgc gtttatttag tgccgcgcgt tgccatcaac ttcttctatt
    19021 atcctgacga taaaatttac ggtcccgacc cctggtcggc ggaatccgtc gaatttacgg
    19081 ctaaggacgg tactcgtctg caaggctggt ttattccttc ttcgacgggc cctgctgaca
    19141 acgccatcgc aaccatcatt catgctcacg gcaatgccgg aaatatgtcc gcccactggc
    19201 cgctggtcag ttggttaccc gagcgtaatt tcaacgtttt tatgtttgat tatcgcgggt
    19261 ttggtaaatc aaaaggcacg ccgtcccagg ccggattgct ggacgatacg caaagcgcca
    19321 tcaatgtggt gcgccatcgc agtgatgtaa acccacaacg gctggtgctg ttcgggcaga
    19381 gtattggcgg ggcgaatatt cttgctgtta ttggtcaggg tgatcgtgaa ggcatacgtg
    19441 cagtgatcct cgactccaca tttgcctctt atgcaaccat cgccaaccag atgatccccg
    19501 gcagtggcta cttacttgat gagagttaca gcggcgaaaa ttatatcgcc agcgtcagcc
    19561 cgatcccgct tttactcatt cacggtaaag ctgatcacgt tatcccatgg cagcacagcg
    19621 aaaagttgta tagcctggca aaagagccaa aacggttgat cctaatcccg gatggcgaac
    19681 acattgatgc tttttccgat cgtcacggcg acgtttatcg cgaacagatg gtgaacttta
    19741 tccttagcgc gttgaatccg cagaactaac ccatgatcgc tagcacgata atcattcaca
    19801 aaatcacctt aagacatgct aatccactgg tcaggacagt ttaagatgag aaaaattctg
    19861 tgacgcttgc caacatttct gatgattagc attcccttcg ccatttccct gagcaaactt
    19921 tagctattct tatcaattat gcttatggga gatctacaga tgatgcctac gcttgctcca
    19981 ccatctgtcc tttcggctcc ccagcgccgc tgccagatct tgctgacgct ctttcagccg
    20041 gggttgaccg ccaccatggc aaccttcagc gagcttaatg gtgtggatga tgatattgcc
    20101 agtcttgata tcagcgaaac aggacgggag atcctgcgct atcatcaact cacactgacg
    20161 actggttatg acggtagcta ccgggttgaa ggtacagtgc ttaaccaacg tttgtgttta
    20221 tttcactggc tacgacgtgg tttccgtctg tgtccgtcat ttattaccag ccaattcacc
    20281 cccgccctga agagtgaact gaagcggcgc ggaattgcgc gtaactttta cgacgatacc
    20341 aatctacaag cgttagtgaa tctctgctcc cgacggctgc aaaaacgctt tgaatcgcgc
    20401 gatattcatt tcctgtgtct gtatctgcaa tattgtttgc tgcagcacca cgctggaatt
    20461 acgcctcagt ttaatccgct ccaacgtcgc tgggcggaat cctgccttga atttcaggta
    20521 gcgcaggaaa ttggacgcca ctggcagcgt cgggcgctcc agcctgtacc acctgatgag
    20581 ccactgttta tggcactact tttttccatg ttgcgggttc ccgatccatt gcgggatgcg
    20641 catcagcggg acagacaatt gcgtcagtct atcaaacgtc tggtaaacca ttttcgtgag
    20701 ctgggaaatg ttcgttttta tgatgaacag gggttatgtg atcagcttta tacccacctc
    20761 gcccaggcgt taaatcgcag tttgtttgcc atcggtattg ataataccct gccggaagag
    20821 ttcgccagac tgtatccacg cctggtacgc accacccgcg cggcgctggc cggatttgaa
    20881 agtgaatacg gcgtccatct ttctgatgag gaaagtggtc tggtcgcggt gattttcggt
    20941 gcctggctaa tgcaggaaaa cgatctgcat gaaaaacaga ttattctact gaccgggaat
    21001 gatagcgagc gagaagcgca gattgagcaa cagttacgcg aactaacgtt actgccgctc
    21061 aacattaaac atatgtcggt aaaggcattt ttgcagacag gcgctccgcg tggcgcggca
    21121 ctgattattg ctccttatac catgccgtta ccgctctttt caccaccgct gatctatacg
    21181 gacctgacac tgacaacaca tcaacaggag cagatccgca aaatgctcga atcagcatga
    21241 gggaacaact ttcgggcgca aaaacattgc cggaagcgcc accagcgcca tcacccagaa
    21301 cacgccgtgg cccagatatt gatacaggaa accggcgaaa acggtcatga tagcgatact
    21361 gccgcccatc gcgacggcag agtaaaccgc ctgtaaacgg atgacttcgc taccctggcg
    21421 agcagcaata tagcgcatgg cggccaggtg gcagaccgtg aaggtgccgc aatgcagaat
    21481 ttgcaccact atcaaccacg gcaacgccgt agttgctccc ataatgcccc agcgcactac
    21541 gccgcaaatc gctgagatca acagcatatc gcgtgcacta caacggcgga aaagtttatt
    21601 actcagtgca aagataatga cttccgccac cacgcccagc gaccacaaat accccaccgc
    21661 cgaggccgag tagccagctg cctgccagta aatggcgcta aaaccgtaat aggccgcatg
    21721 tgccccctgc aataaacaaa cgcaggccag aaagcgccag ttctggcgaa ccagcgccaa
    21781 ccacgctgac caaccggtgc tctcctgctg gcggcttgcc ccttgtggct gaatcgtcgg
    21841 acggatgaga aagccgagca gcatggatgc cacgcccaac gtcaacagca cgaggatcac
    21901 ccgataatca aacatagtga ccagtttgcc cgtcagcgcc gagccaatga caaacgccac
    21961 cgagccccac agtcgcactt tgccgtaatc aagcgggaac tgcttttgcc acgtattcgc
    22021 cagtgcatcg gtcaacggta ccagcggtga gaaaaagagg ttaaagccaa tcatcaccag
    22081 catcagccac gctacgtgcg cccccgccca gaaggcgaca gcaaagagaa gtgtcagcag
    22141 tgccagcacg cgcaaggcgg aaatcaggcg agaaggatcg ctgacgcggg gcgcgatgag
    22201 caaactcccg agaaaacggg caaccagacc tgcccccaat aacaggccga tggtttctgg
    22261 cgttaaacca atccctttaa gccagacgct ccagaaaggt agaaaaatgc cgtaactaaa
    22321 aaagtatgtg aaatagccga gcgccaacca gcgcgtggat tgcaaaacca tgagtccctc
    22381 ccgtcaggag gcgttagtct ggagttaatc taatgttggt gcaagttgaa aaataagaat
    22441 attcgagcct gcttcccgat gtctgtaacg ccagttcaca agcactcaaa aactgcgatc
    22501 cgtgacgcat gttgcaaaga taatgcgtaa ttatgccgtt acgcttgcca aacgttcctg
    22561 cacaatggcg atgaaatcgc gtaatgccgg cttcatctcc cctttcttcc acgccatcag
    22621 taaagcaatg gaaggcacat ttccggcaat gggacgaaaa acaacctgcc cggtattaaa
    22681 attattcata taaccgggta tcaaagtgac gcccagcccc atgcccacca gattcatggt
    22741 caccagaata ttcgttgcca cctggacgat atttggctgg ctgttttctt gtgcaaacca
    22801 cgctttaacg atcggcgcaa gcgaaccgga atacgccgga tcggtactga cgaaattcac
    22861 gccatccagt tgcgcggcgg tgatctcttt ttcatgtgct aaagggtgat caaccggtaa
    22921 caccaccact aatggttcgt caaaaagctc cagataatca atctccgggc tataaacagg
    22981 atggcgcatt aagccgacgt cgagttcacc acggcgaatt ttttcctcct gttgcgtggt
    23041 gattaaactc accagctcaa tcaaggtgtc tggctgtctg agacgaaaca tcggtaatac
    23101 ttttggcagt aaattcactt ccgccgatgg cacaaagcca atggttaatt gtctgtcttc
    23161 ctgaacaatt ttccgcgccc gtaatttggc attttccgct tgttcgagga ttgccagcgc
    23221 gtcctggaga aaacattctc ccgccgccgt caacgcgact ttgcgcttat ccctcaccag
    23281 taacggaaca ccgacacagt tttcaagatc gcggatctgg ctgcttaacg aaggctgtga
    23341 ggtatgcagt ttttccgccg cacgggtaaa gttcagtgcc tgcgccactg cgacgaaata
    23401 gcgtaaatgc cgtagttcca tcaccttccc cttgttatcg aaaaaacgtc tcagctggta
    23461 gaaattaact atttcacata ttagcaacca accagcaaca tccttatgac acaaaaatag
    23521 aaggtcaata catcttatct ttcaggatta aaaaatatga ccacaccctc agatttgaac
    23581 atttaccaac tgattgacac ccaaaacggt cgggtcactc cgcgtattta taccgacccg
    23641 gacatttacc aactggagct tgagcgtatt ttcggtcgtt gctggctatt tctcgcccac
    23701 gaaagccaga tcccaaaacc cggtgatttc tttaacacct acatgggaga agatgcagtt
    23761 gtcgtggtgc gccagaaaga cggcagcatc aaggcgtttc tcaaccaatg ccgccaccgg
    23821 gccatgcgtg tgagttatgc agattgcggc aacactcgcg cctttacctg cccgtatcac
    23881 ggctggtctt atggcattaa cggcgagttg atcgatgtac cgctggaacc tcgcgcctac
    23941 ccacaagggt tgtgtaaatc ccactggggg ctaaacgaag ttccttgtgt ggagagttat
    24001 aaagggctga tttttggcaa ctgggatacc agcgcaccgg gcctgcgtga ttacctgggt
    24061 gacattgcct ggtatctgga tggcatgctg gatcgtcgcg aaggcggcac cgaaattgtc
    24121 ggcggcgtac aaaagtgggt gatcaactgc aactggaaat tcccggcaga gcagttcgcc
    24181 agtgaccagt atcatgctct gttcagccat gcttctgccg ttcaggtatt aggggcgaaa
    24241 gatgatggca gcgataagcg cctcggtgat ggacaaaccg cccgcccggt gtgggaaacc
    24301 gccaaagatg ccctgcaatt tggtcaggat ggtcacggca gcggcttctt ctttactgaa
    24361 aaaccagatg ctaatgtctg ggtcgatggc gcagtttcca gctattaccg cgaaacctat
    24421 gccgaagctg aacaacgttt aggtgaagtt cgcgccctgc gcctggcggg tcataacaat
    24481 attttcccga cactttcctg gctcaacggc actgccacac tccgcgtctg gcatccgcgc
    24541 ggccctgatc aagttgaagt gtgggcgttc tgtattactg acaaagccgc ctccgatgaa
    24601 gttaaagccg cttttgaaaa cagcgccact cgtgcttttg gtcctgctgg ttttctcgag
    24661 caggatgact cggagaactg gtgtgaaatc cagaaattgc ttaaaggcca ccgcgcccgc
    24721 aacagcaaac tgtgtctgga aatggggctt ggtcaggaaa agcgccgcga cgacggcatt
    24781 cctggcatta ctaactatat cttttcagaa actgctgctc gtggaatgta ccaacgctgg
    24841 gccgatcttc tgagtagcga aagctggcag gaagtgctcg ataaaaccgc cgcttaccag
    24901 caggaggtga tgaaatgagt gcgcaagttt cactagagtt acatcaccgc attagccagt
    24961 ttctctttca cgaagccagc ttactggacg actggaaatt tcgtgactgg ctggcacagc
    25021 tcgacgaaga gatttgttac accatgcgca ccacggtgaa cgcgcaaacg cgcgaccgcc
    25081 gcaaaggtgt tcagccgccg acaacctgga tttttaatga caccaaagac cagctggagc
    25141 ggcgaatcgc ccgtctggaa acgggcatgg catgggcaga agagccgccg tcacgcaccc
    25201 gccacttaat cagcaactgc cagataagcg aaaccgacat cccaaacgta tttgctgttc
    25261 gggtgaatta tctgctttat cgggcacaaa aagagcgcga tgaaacgttc tatgtcggga
    25321 ctcgttttga caaagttcgc cgtctggaag atgacaactg gcgcttgctg gaacgggata
    25381 tcgtcctgga tcaagcggta atcacttccc ataacctgag tgtactgttc tgatgaatcg
    25441 aatttatgcg tgtcccgttg cggatgtgcc ggagggtgag gctctccgga tcgatacctc
    25501 gcccgtcatc gccttgttca acgttggcgg cgagttttat gccattaacg atcgttgcag
    25561 ccatggtaat gcgtcgatgt cagaagggta tctggaagat gacgccacgg tggagtgccc
    25621 gctacacgcc gccagttttt gcctgaaaac ggggaaagcg ttatgcctgc ccgccaccga
    25681 tccgctcacc acttatccag tacacgttga aggtggtgac attttcatcg acttaccgga
    25741 ggcgcagcca tgagcgatct gcataacgag tccattttta ttaccggcgg cggatcggga
    25801 ttagggctgg cgctggtcga gcgatttatc gaagaaggcg cgcaggttgc cacgctggaa
    25861 ctgtcggcgg cgaaagtcgc cagtctgcgt cagcgatttg gtgaacatat tctggcggtg
    25921 gaaggcaacg tgacctgtta tgccgattat caacgcgcgg tcgatcagat cctgactcgt
    25981 tccggcaagc tggattgttt tatcggcaat gcgggcatct gggatcacaa tgcctcactg
    26041 gttaatactc ccgcagagac gctcgaaacc ggcttccacg agctgtttaa cgtcaacgta
    26101 ctcggttacc tgctgggtgc aaaagcctgc gctccggcgt taatcgccgg tgaaggcagc
    26161 atgattttca cactatcaaa tgccgcctgg tatcccggcg gcggtggccc gctgtacacc
    26221 gccagtaaac atgccgcaac cgggcttatt cgccaactgg cttatgaact ggctccgaaa
    26281 gtacgggtga atggcgtcgg cccttgtggt atggccagcg acctgcgcgg cccacaggcg
    26341 ctcgggcaaa gtgaaacctc aataatgcag tctctgacgc cggagaaaat tgccgccatt
    26401 ttaccgctgc aatttttccc gcaaccggct gattttacgg gtccgtatgt gatgttggca
    26461 tcgcggcgca ataatcgcgc attaagcggt gtgatgatca acgctgatgc gggtttagca
    26521 attcgcggca ttcgccacgt agcggctggg ttggatcttt aaggaagtac gatgaaagaa
    26581 aaaacgatca ttattgtcgg gggcgggcaa gcggcggcaa tggctgcggc ctcgctacgc
    26641 cagcaagggt tcaccggtga gctgcatctg ttttccgatg agcaacatct tccttatgaa
    26701 cgccctccgc tctcgaaatc catgttgctg gaagattccc cacagttgca gtctgtgtta
    26761 cccgctcact ggtggcagga aaacaatgtt catctgcatt ccggtgtaac catcaaaaca
    26821 ttgggccgcg acacacgaga gttagtgtta gccaacggcg aaagctggca ctgggatcag
    26881 ctttttatag caaccggcgc ggcagctcga ccgctgccgt tgcttgatgc actgggagaa
    26941 cgctgcttta ccctgcgcca tgccggtgat gccgccagac tgcgagaagt tctgcagccc
    27001 gaacggtcag tcgtgattgt cggtgccgga actattggtc tggaactggc tgccagcgcc
    27061 acgcagcgca gatgtaaggt gacagtgatt gaactggcgg caaccgtcat gggccgtaat
    27121 gcaccaccgc ccgtgcaacg ctatctttta cagcgccatc agcaggctgg tgtgcgcatt
    27181 ctgctcaata atgccattga acatgtggtc gatggtgaaa aagtagaact gacgctgcaa
    27241 agtggcgaga cgcttcaggc cgatgtggtg atttacggta ttggtatcag cgccaacgac
    27301 caactggctc gcgaggccaa ccttgatact accaatggca ttgtcattga tgaggcttgc
    27361 cgcacctgcg atcccgcgat ctttgccggt ggcgatgtgg caatcactcg tcttgataat
    27421 ggtgcactac accgctgcga aagctgggaa aacgccaata accatgcgca aattgccgct
    27481 gccgcaatgt tagggctacc gcttccgcta ctgccgccgc cgtggttctg gagtgatcag
    27541 tacagtgata acttacagtt tattggcgat atgcgtggcg atgactggct ttgtcgtggc
    27601 aacccggaaa cccagaaagc gatttggttt aatctgcaaa acggcgtgct tatcggtgcg
    27661 gtaacgctga atcagggacg tgagattcgc tcaatccgca aatggatcca gagcggcaaa
    27721 acgtttgatg cgaaacagct gacagatgag aacatcgcgc ttaaatcact gtaaccagga
    27781 taattagcga atatctcaac gcctggggcg tggcgaggta ccagagtgtg tattacgttt
    27841 aaatcacatt atcttgcaaa gggattggtt atgaactcac tacgttattt tgattttggt
    27901 gctgcccgcc ccgttttgtt attaattgcc cgtatcgccg tggtcctaat tttcattatt
    27961 tttggttttc ccaaaatgat gggctttgac ggtacggtcc aatatatggc ctcgctgggc
    28021 gcaccgatgc cgatgctggc agcgattatt gcggtagtta tggaagtgcc cgccgcgata
    28081 ttaatcgtgc ttggcttttt cacccgtccg ctggcggtac tgtttatttt ctacacgctg
    28141 ggtacggcgg tgattggtca ccattactgg gatatgaccg gcgatgccgt cgggccaaat
    28201 atgattaatt tctggaagaa tgtcagtatc gctggcgcgt tcttgctatt ggcaattacc
    28261 gggccggggg caatttctct cgatcgccgt taggaaagat gccggatgcg gcgtgaacgc
    28321 cttatccggc attttataaa ttacagccac tctacccgca acgacatctc tgaggttgtt
    28381 gattcccccg gcgcaagcgc aatgaggtca cccccttccg ggcgatgatg gtcatccggc
    28441 gcatggctca tcggctccag acagaagaaa tcaaacgcat atcctttatc aaacgccggg
    28501 tcggaaacaa agatgaaata acacggtgca ggtggcgtcg tttctatgat gatggcataa
    28561 ccctcctgcg gctgctcgat gcgggcctga ccattccatc cggcaaaacc attgttcacc
    28621 cactggcgcg gcaacggcgc tagctggtta aaatccagtt cttgcggtag ctgctcgcaa
    28681 aactcacccg ccagccactg ctcccgctcc agccagtaac cgctcgcctg tgcctgaatc
    28741 cgcgtttgcg gcgacaacgg aaaataagga tgccagccgg taccaaatgg cagcgtctct
    28801 gccccttgat tggtgacaga gagcgtcacc gtcagcgtat ccgccgttaa atgaaacgcc
    28861 tgacttaccc gatagtgata gacaccgctg cgatgttcat acaccagaca caaactatca
    28921 tcgctgtgcg agacacattg ccactcgccc agccagccat cgccgtgtag atagtgcgca
    28981 tcccactcaa cattcggttg cagttgatac tcacgcccct gccagacaaa acggttgccg
    29041 ctcacccggt ttgcaaatgg caccagcgga aaacaagagg catcggttgc cacaccgctt
    29101 tttttaccag gacgtaagag cggcgtcgta tcgcgccaga agccttcgat tacgccgccc
    29161 tggtcagaaa cgcccagctt tagcgacccg tgggataagg tatagatggt catccgctaa
    29221 ctccttaatc cgggaagtta atcacaactt tcccgcattt gccgctcgcc atcagcgcat
    29281 aggcatcacc tgcctgttcc agcgagaagc gatgggtaat ggcgttacgc ggccacagct
    29341 tccagtcagt caaatcgtgg gcgcattttt ccatatggaa cagactggtc acccaggagc
    29401 caataatccg ccgttgatgg tgcatcagat cggcgctgac ctcgaattcc acttttccgg
    29461 tttcaccaat gtaaaccacc cgcccccagt cagcggtgga ttgcagtgcc agtaagcgac
    29521 ctgcggcatt accggaacaa tcaagcgcaa catccgcgcc gccgtgagtg agttcggcga
    29581 ttatctgcgg cagaccttca gtggtcgcta aatagccgtg atccatcact cctaactgtt
    29641 ttgccatcgc cagacgttcc ggcagcatat caacgccgat aatccgtttt gcaccgcgac
    29701 ctttcgccag catcatcccc atcatgccga ctggccccag accgaccacc agcacgttat
    29761 cactgccgga aacttcgccg cgcaaaatcc cttcataggc cgtgccaacg ccgcaactga
    29821 taaacgcacc atcttcgtag ctcagcgcat ccggcaggag gatcagatct ttttcttccg
    29881 ccagcaagta ttcggcatga ccgccgtcac gctgccagcc gtaagccgct tttccttcgc
    29941 cagtacaaga aataggaaaa ccgcgacggc agttcgggca aaaaccacag ccagaaatgt
    30001 gatacaccag cacgcggtcg ccctctttaa aatggcggca gccttgcccc atcgccacaa
    30061 tctgcccgca cggttcatga ccgttgataa atccctagta caacggttta tcggatgccg
    30121 ccgccgtagc gcggtgctgg tggtagatat agtggacatc gcttccgcaa attccggagg
    30181 atttcatttt gatcagtacc tggttaatcc ccggcgtcgg cactgcaact tcccgcagat
    30241 cgacggtcga atttcctggt aaataagctg ccagcatcgt tttcataaat ccctcattaa
    30301 caatacgatt aattttcatc cctgccgcac ccgcgccagg ggcgttaagg tttagcgttt
    30361 cgctttactg ctacgctggg tcagcaagat attcgccagc accgccacca cgatgatgac
    30421 gccgcgcacc acctgctgga aaaaggagtt aatcccgagc agcaccagac cgttaccgat
    30481 tagcgtaatc accagtacgc caagcagcgt accgaacaac gagccgcgac caccggaaag
    30541 cgccgtaccg ccgaccacga ccgcggcgat gacgtcaaac tccagaccgt ttgcggcacc
    30601 tgcgttaccg gaaccgaggc gcgccgccaa caaaatgccg gtcaccgccg ctaataatcc
    30661 cgaaagggta aagataagaa tgcgtacccg acgaacgtta ataccgcaca actgcgccgc
    30721 cgtggcatta ccgccaacgg caaaaaccga gcgcccgaag gcggttttgc ggctaatgaa
    30781 cacaaacagc gcaaacagca ccatcatgat cagcgcggat accggcacac cgagaaattg
    30841 tccgcccagc cagtccagca cctcgttttc gtcaatcggc actggcagag cgttggtcat
    30901 aaacaagccc attccgcgca gggcgctcca cagccccagc gtggccacga aacttggcac
    30961 gttaaacacg ccacgcagca ccccggcgag cgttcccatc agcgcgccta acagcaacac
    31021 cagcagacaa gcaatcgcca acggaacttc aaattgcagc aaaaatgcca ggcataccga
    31081 gacaaaagcc accatcggcc caacgctgac atcaatttca ccggagataa taatcagcgt
    31141 catcgcccag gcggcaatcc caatggtggc ggcatcgcgc agcacgttca tctggttatt
    31201 caatgagata aagccaggcg cgttcaggga gaagaccaga taaagaatgg caatcaccac
    31261 cagcaaaccg atctcattaa tatggtgact gacaaattgt ttgagcgaga cgctcttgcc
    31321 ctgcggcaat ggtaatgacg aagcagacat cttcaggttc ctcggtaaaa ttcatcagtg
    31381 cacagacaga atggcggaca tcagctcatc cacattgacc ggagcgtgaa actcctgcga
    31441 gaacgtgccg tgctgtaata acaggatgcg gtcacacacc aacggcaact cctccacttc
    31501 actggagata aacaccacgc tttttccttc ggcagccagc tcacggacaa tacggtaaat
    31561 ctgctgtttg gcttcgatat cgacgccgcg cgttggctcg tcgagcaaca aaatctggct
    31621 ggcggcatag acccaacgac cgatcactac tttttgctga ttgccgccag aaagcgtgcc
    31681 gatgggcgtt tcgctactgg cggccttgac cgtcatccgc tgcatcactt cttcggtcag
    31741 gcggcggatg gtggaccatt gcagcactcc gttggcgctg attttttgcc gattggtcag
    31801 cactgtattt tcgtcaacgc ccaaccaggg aatgatcccc gcttctttgc ggttttctgg
    31861 cgtatagcca atgccgcgtt tcagcatgtc gccgtaatcg gggcgcgtga ttttctcgcc
    31921 gttgataaca atttcgccct gttcatacgt ctccagccca acaatcgcct tcagcaattc
    31981 actgcgccct gcccccagca gaccagcaat gccgagcact tcgccacgac gtagcgtaaa
    32041 gctgatatcc tccagcttgg gcttatggcg taacgcacgg acttccagca cggcctgatc
    32101 cacaatttcc tgaggtgcaa ccggcgcaat atcaacgtga tcgcgcccta gcattagcga
    32161 cacaatatga tgcgtggaag tgttttcgag catcacatcg ccagccacct gcccgtcgcg
    32221 cataacggtg gcactggagg caatgcggcg aatttcttcc atccggtggc tgacataaat
    32281 caccgccacg cccagtgctg acatcttttt caccgctctg atcaccagtt caacttccgc
    32341 actcgcaagc gaactggtag gttcatcaag aatgaccacg cgcggctcgc ccttcatcac
    32401 ccgcgcaatt tccaccagct gcttatgcgc cgggcttagt gttgaaacaa gttgttcagg
    32461 actaacgtca acgcccagcg cctgtaagca acgttgggca tcctgcgcca tttgcaggta
    32521 atcaatcatg ccgttgcggc ggggccactg accgaggcag aggttttccg ccaccgtcag
    32581 cccttccacc agacttaact cctgataaac cgcgcgtacc cccagttcag cggcacggcg
    32641 agtcagcgta gcttcgtcac cttccagtcg cgtctcgcca atccagattt caccgctatc
    32701 cggacgttca ctgccggtaa gcattcgaat gagggtcgat ttgcccgcgc cgtttttgcc
    32761 taacagcgca cgaacctcgc ctttattgag cgtgaagtta acgttatcca acgcaacgac
    32821 gccgggataa cgcttatttc ctgccaccac ttttgctacc gggactgcct ctgttgccgt
    32881 gaacataggg acctctgcga atcagcgatt agggcagacc atcaacgtgc gttgccagcc
    32941 actgtttacc gtcttccgtt ttggtataga gatcgatagg cacctgaatc actttttcac
    33001 cgtcggcttg tttattgata accttcaatg tttgcgcgaa aacagcattg cccattttct
    33061 taccggaaat atccactacc gctttcagca cctgattgtt ttccagctcc tgagcaattt
    33121 cggttgtcat atccgaaccg aaaacagcaa tttttccggc ctgattttga ttacgtaccg
    33181 cttttaccgc gccgagtgtc gcaccgcccg attcccccat aatggcgttg agatccggcg
    33241 tggagataat cagtttttca ccaacggaaa tcgctttatc taaaacagtc ccttcctgat
    33301 tagcgacaat ttgcgcgccg ggaacgcggg attttaatac ttcttcaaat cctttacgtc
    33361 gctgcacaca aacttcaaag gcttcgcaat tgatgacggc aattttcggc tggtcaattt
    33421 tattggcaat aaaataatcg gcggcagcgt tacccagttt tttaccaaat tccagcgggt
    33481 cacccaccag atacgccgag acatatttat cgaccccctt ttgattaata caggtgttgt
    33541 agcaaatcac cggaataccc gcttcactgg cgcgacgaac ggtacggcta ctgccatttt
    33601 cagacactgc cgataaaata atagcatcga cattacgcgc cacgagggta tcaacaaagg
    33661 tactttcttt cgaaatatca ccctgggcgt tagtttcaat taactgtacc tgtactgaag
    33721 aatcttttgc cgcatcctga actccctgac gcactccagc gtaatatccc tgagtatcaa
    33781 ggtatattgc gccaatggtc atttctttat ccgcagctct ggcaaatagt gcgctaccta
    33841 atagcgtagc cattaataat agattacggg ttgttctcat ttttttaggc atagagcctc
    33901 ctgtagggtt tttattaaca acggcttatt ctaattattt tgtgatgagc ggcagcgcgt
    33961 gccgctcaaa tattacagtt aatagggttt agtgaacgta gttaaaaata aaaggcatca
    34021 cggtggtgaa taatgccgct ttcggccagg ccggattaat cgccgtcaat tcagcgcgag
    34081 cagattcgta ttgcgcaatc tcgcctaatc ccgcggccgc cagcatcgcc accatcaggc
    34141 atttttcttt atgctgctgt tgtaaatcgc catacagcga caacaagtca ggctgcgaga
    34201 cggcaaagaa atccgcttcg atactggttt tcgccatctc ttgcgcccac tgtttcattt
    34261 cactaaacag ttgctgtgcg gtgtgttgtt cgcccagtaa tcgcagcgcc attccttgcc
    34321 agaagaggta atcaaccggc tgatcgttgt aatagctgtg gatgttaatg gtgcgatcgc
    34381 cggtcgccgc cagatgtaaa cagcgcgtcg cttcagtttc atcgccctgg gctttggcgc
    34441 atatcgcctg ccagaaccag atgtcgttat cagtttgccc cggtaaacgg ccttcgctta
    34501 aattctccgg ataatgcagc gcggcatgaa gcagttcgct ggcctgctgc ggctctctgg
    34561 catcaagatg ctgccaggcg cgtaataact ggttgaggat aaactgactg gtgaccttcc
    34621 cttccccgcc ttcccacggg tggaatttgc gcgtggcgag aatgtccgcc gctttgtctg
    34681 cctgccccgt gagatgccac aaattgagca gttctgcggt catgtcgtcg cgtttcagcg
    34741 caatttccag attattttcc agacgcgcca gtcgtttctc cggtgtggtt ccacttagct
    34801 tatcaagcaa atcccgttcg aaaagcagac gtgcatcctg cggcgcaagc tgataagcat
    34861 tatcaagata acgcgcggcc agctcataat cgtgttgctt attccacgca tggatcgcta
    34921 acccgcgcca gccgtcggca aactccggcg acatctctac gcaacgttgc cataaggcaa
    34981 tggcttcgtt gtagctacgt ttgttgtagt agaagcaggc cagtaaatgg cgagcaaacc
    35041 agcactcttc aatactctcc agcgccgcca cttcttccag cgtattcggg aaacggacaa
    35101 actgcgggaa gacatcaatg gcttttgcga ccagttcgcc acgttcggct ttcggcagca
    35161 agctggcttg caggtaaagc ggcagcgtgc gctggcagtc cagtgcattc agcatctctg
    35221 ccgcgagggt gggcattccc cagttaatca gttgcccggc ggtcatcagg gcgttaacgt
    35281 cgcgtccctg acacagaccg cgccactgcg cgagagctga ttcgctacga ccatcgaacc
    35341 agttcaggca ccacagagtg gcgttcagcg gataatcgcg cagcagtttc tcgcgctgca
    35401 aacgcgcgtt gtcctgacga ccacttaaca ccagcagcag attatgcagg caaagcactt
    35461 cctgattgat tgggcaggcg cgaagacttt gttggcaaaa atccagaccc gcgtcgaagt
    35521 taccattacg cgccgccagt cgtgccagac cataatagcc tccggcttta ctgttgccgc
    35581 tccagaccgc acgccagaaa tcctcttcgg cttgttgata ttgtccctga cgttcgtaag
    35641 cactggcgcg aatcaaactc gcctgtccgc actgcggatt tttgttcagc gcatgtgcgc
    35701 gtttcagagc ctgagtggca tacgccaccg cttgcgggaa atctgcgcgg ttatactcca
    35761 gcatcgccag cgccagatta cagcggtaat ccagcggatc cagcgccacg ccgcgcaggt
    35821 agtaatcgaa cggtgaacgg ctggcgtgat gatattgctc cagatgctga ccgataaacc
    35881 aggcttcatc tgtactggta atgtcttgtg ctgacagtgg cgctttggcg acgtccggca
    35941 acggcaactc ttgcggctga tgttcctgat aactcagtac aatattgccg tcggcatcag
    36001 agagctcaat cgtcagtctt tcaggattga taccgtgtaa cacgccctgg atggcggtcg
    36061 ctggtgtcag ggccacggca tcatcgagca acgcgtcgca tttgccgatt tcgcggatcg
    36121 ccaggcggta tccgttcaac ggagagatgg catacagccc ccactcaatc ccccgctcac
    36181 tacgctggag tttgattacc gcatcgcggg aggcgttttg caccatgccc agagaatggt
    36241 aaggcaggaa aaactgctca aaacgcttct cttcgtaagc atcaagccgg gtaaaatcag
    36301 gctggttatc ggcaaaaata ccggtcatca gttcgatata cgggccgtta ttatcagtta
    36361 ggctcttatc ccacgcctgg ccaaattcac tgtgtcccca gctccactgc tttttacctg
    36421 gcgcaatatg gtggttggca acgtgtagca aaccgccatc ttcatcgtga caccacgcgc
    36481 caacaaaatc gtactgtgat ttttcagcca tatatgaggt tggaacgggc acatttttat
    36541 agcgagaaat gtccactcca gcggagtagt ccactttgta gtaagtgccg gtggcgatag
    36601 ggaaagcgga gacggcccgt ttgccgtgat caaacaccgc cgttacatcc ggcggaaaga
    36661 cgctctgatg cccttcaccc cctttcactg ccgggttggc ccaccacaag aaatgacgcg
    36721 gcgtggcgtt gccgttatag acgcggctgg cgatttccag cgccgcccgg tcagggcgca
    36781 gggtgaaacc tgtcatcacc tgtaaaccat gcatcggctc cgtttcgcct acccacaccg
    36841 tctgtgcgcc gtcttcatgg gcttcgaggg tgaaatcaac gggcataaag gtggtcgggc
    36901 gatggtgttg cggccagtta aactcaatcc cgccggagat ccacggtccc agcagcccca
    36961 ccagcgcagg tttaatgact tcattgtgat agacaaaatc gcgctgtttc actttatccc
    37021 atgcgcgatg cacccgaccg cccaattccg gcaggatcat cactttgatg tagtcgtttt
    37081 ccagccacac cgcctgccag gatttcaggg ttttctgctc gctcagcgta tcggtcacgc
    37141 cgtagggata aaccgcgccg gacgatccct gataaacgcg attttccagg aacatgggat
    37201 gtatatcctg cggcccggtt tcataggtcg ggatctcaac gcgctcttgc cacactttta
    37261 ctggagtcat ggtgccctca atattaacaa gacatactga attaaaagat ttgtggcagt
    37321 gtattgaaca atctggcaat gttttcgcgg aataatcacg caattaacta aacaaggttt
    37381 agtgaagatg agagcctgca ttaacaatca acagattcgc caccataaca aatgcgtgat
    37441 tctggaactg ctgtaccggc aaaagcgcgc caataaatca acgctggccc ggctggcgca
    37501 aatttcgatt ccggcagtca gtaatatttt gcaggaactg gaaagcgaaa aacgggtggt
    37561 gaatatcgac gatgaaagcc agacgcgcgg gcatagtagc ggtacatggc tgattgcgcc
    37621 ggaaggtgac tggacgctgt gtctgaacgt gacgcccacc agtattgagt gtcaggttgc
    37681 taatgcttgt ttaagtccga aaggtgaatt tgagtattta cagattgatg caccgacgcc
    37741 gcaggcgctg ctgtccgaaa tcgaaaaatg ctggcatcgc caccgtaaat tgtggccgga
    37801 ccgcaccatc aatctggcgc tggcaatcca cggtcaggtt gatccagtga ctggagtgtc
    37861 gcaaaccatg ccgcaagcgc cgtggacaac gccggttgag gtgaagtatc tgctggaaga
    37921 gaagctgggc attcgggtga tggtcgataa tgactgcgtg atgctggcgc tggcggaaaa
    37981 atggcaaaat aattcgcagg aacgggattt ctgcgtgatc aacgttgatt acggcattgg
    38041 ctcgtcgttc gtgattaacg agcaaattta tcgcggcagt ttgtatggta gcggacagat
    38101 tggtcacacc atcgttaatc cggatggcgt cgtctgcgac tgtggacgtt acggctgcct
    38161 ggaaaccgtc gcctcgttaa gtgcattaaa aaaacaggcg cgggtatggc taaaatcaca
    38221 accggttaat actcagcttg atcctgaaaa actgactaca gcgcagttaa tcgctgcctg
    38281 gcaaagtgga gaaccgtgga tcaccagctg ggttgatcgc tctgccaatg ccattggttt
    38341 gagtctgtat aacttcctca acatcctcaa tattaatcag atttggttgt acggtcgcag
    38401 ttgtgccttt ggtgagcact ggcttaatac tattattcgc cagacaggat ttaacccgtt
    38461 cgaccgcgac gaaggaccga gcgtgaaagc gacgcaaatt ggcttcgggc aattaagccg
    38521 cgcacaacag gtgctgggaa ttggctattt gtatgttgag acgcagttac gacagatttg
    38581 atggcgcgat aacgtagaaa ggcttcccga aggaagcctt tttcaacaga ttaaccgttt
    38641 cgcttatgcg taaaccgggt aacgtgcgca gatgtcgaga actttacctt tgatgcgctc
    38701 gataacggct tcatcattga tgctgtccag cacgtcacac atccagccag ccagttcttt
    38761 cgcttctact tctttgaagc cgcgacgcgt aattgccgga gtacccacgc gaataccgga
    38821 ggtcacaaac gggctcttcg gatcgttcgg tacgctgttt ttgttgacgg tgatgttagc
    38881 acggcccaga gcggcgtctg cttctttacc ggtcaggttt ttatcgacca gatcaaccag
    38941 gaacagatgg ttatcagtac cgccggaaac cactttgtag ccgcgttcca ggaacacttc
    39001 taccatcgct ttagcgtttt tagcgacctg ctgctggtaa gttttgaact caggctccat
    39061 cgcttctttc agagcaaccg ctttaccggc gattacgtgc atcaacggac cgccctgacc
    39121 accagggaaa acggcagagt tcagtttttt gtacagctct tcgctaccac ctttcgccag
    39181 gatcagaccg ccgcgcggac ccgccagggt tttgtgagtg gtggtagtaa caacgtgagc
    39241 atgaggaacc gggttcgggt agacgccagc agcaaccagg cccgcaacgt gcgccatatc
    39301 aacgaacagg taagcgccga tgctgtcagc gatttcacgc attttcgccc agtccaccac
    39361 gccggaatat gcagagaagc caccgataat cattttcggt ttgtgttctt tggcttgttt
    39421 ttccagatcg gcgtagtcga tatgaccggt agcatcaata ccgtaaggaa caatgttgta
    39481 cagtttaccg gagaagttaa ccggagaacc gtgagtcagg tgaccgccat gcgccaggtt
    39541 catacccaga acggtatcac ctggttccag cagcgcggtg tagaccgcaa agttagcctg
    39601 ggagccggag tgcggctgga cgttagcgta gtcagcgccg aacagttctt tcgcacgatc
    39661 gatcgccagt tgttcaacga tatcaacata ctcgcaacca ccgtagtagc gtttgcccgg
    39721 ataaccttca gcatatttgt tggtcagctg agaaccctgc gcctgcatta cgcgcgggct
    39781 ggtgtagttt tcggaggcga tcagttcgat gtgctcttcc tgacgtactt tttcctgctc
    39841 catagcctgc cacagttcgg catcataatc ggcaatgttc atttcacgct ttaacatccg
    39901 catctcctga ctcagctaac aataaaattt ttggccttta taggcggtcc tgttggacaa
    39961 cggcgaacag tataaccgaa tcattgtgcg ataacaggtc ttgacaaagg aatttacgca
    40021 aacgattacc ttcaggctac gcaaggcttt ggagaataaa gagcttgcaa ccggaaacgg
    40081 atttcttttc aggtttgtga tgcaaatttt tcacttcatc acattctttc tgaaaaacac
    40141 caaagaacca tttacattgc agggctattt tttataagat gcatttgaga tacatcaatt
    40201 aagatgcaaa aaaaggaaga ccatatgctt gacgctcaaa ccatcgctac agtaaaagcc
    40261 accatccctt tactggtgga aacggggcca aagttaaccg cccatttcta cgaccgtatg
    40321 tttactcata acccagaact caaagaaatt tttaacatga gtaaccagcg taatggcgat
    40381 caacgtgaag ccctgtttaa cgctattgcc gcctacgcca gtaatattga aaacctgcct
    40441 gcgctgctgc cagcggtaga aaaaatcgcg cagaagcaca ccagtttcca gatcaaaccg
    40501 gaacagtaca acatcgtcgg tgaacacctg ttggcaacgc tggacgaaat gttcagcccg
    40561 gggcaggaag tgctggacgc gtggggtaaa gcctatggtg tactggctaa tgtatttatc
    40621 aatcgcgagg cggaaatcta taacgaaaac gccagcaaag ccggtggctg ggaaggtact
    40681 cgcgatttcc gcattgtggc taaaacacct cgcagcgcgc ttatcaccag cttcgaactg
    40741 gagccggtcg acggtggcgc agtggcagaa taccgtccgg ggcaatatct cggcgtctgg
    40801 ctgaagccgg aaggtttccc gcatcaggaa attcgtcagt actctttgac tcgcaaaccg
    40861 gatggcaaag gctatcgtat tgcggtgaaa cgcgaagagg gtgggcaggt atccaactgg
    40921 ttgcacaatc acgccaatgt tggcgatgtc gtgaaactgg tcgctccggc aggtgatttc
    40981 tttatggctg tcgcagatga cacaccagtg acgttaatct ctgccggtgt tggtcaaacg
    41041 ccaatgctgg caatgctcga cacgctggca aaagcaggcc acacagcaca agtgaactgg
    41101 ttccatgcgg cagaaaatgg cgatgttcac gcctttgccg atgaagttaa ggaactgggg
    41161 cagtcactgc cgcgctttac cgcgcacacc tggtatcgtc agccgagcga agccgatcgc
    41221 gctaaaggtc agtttgatag cgaaggtctg atggatttga gcaaactgga aggtgcgttc
    41281 agcgatccga caatgcagtt ctatctctgc ggcccggttg gcttcatgca gtttgccgcg
    41341 aaacagttag tggatctggg cgtgaagcag gaaaacattc attacgaatg ctttggcccg
    41401 cataaggtgc tgtgatttga tgttgccgga tggaaacatc cggcaaccct tgacgcggtt
    41461 taaattgccg cgtcgtcctc ttcaccggta cggatgcgaa tgacccgtgc cacgtcaaag
    41521 acgaagattt taccgtcacc gattttgccg gtttgcgccg tgcgaataat ggtatcgaca
    41581 caggtatcga caatgtcgtc cggtacgaca atctcaattt tcactttcgg cagaaaatcc
    41641 accatatact ccgcgccgcg gtacagctcg gtatggcctt tctggcggcc aaaacctttc
    41701 acctcagtga ccgtcatgcc ggtaatgccg acttcagcca gtgcttcgcg gacatcgtcc
    41761 agcttgaagg gtttaataat cgcatcaatc tttttcatgc tattccttga aaaggtcgcc
    41821 tgtcttttga tctgctaaac gtaacacata acgccaattc attccttgaa atcgtttgca
    41881 tccagctcgt gtcgggaaag cagtttataa aattctgtcc ggttgcgccc cgccattctc
    41941 gccgcgtggg tgacgttgcc tttggtaatt tgcagcagct tacgcaaata gttgagttca
    42001 aactgattac gtgcctcaac aaaggttggc agcgccgtat tttcaccctc cagcgcctgc
    42061 tctactaatg catcgctaat caccggagat gaggtcagcg ccacgcactg ttcaatcacg
    42121 ttgaccaact ggcgcacatt acccggccag ctcgcggtca tcaggcgttt catcgcatcg
    42181 gtggaaaacg cgcggacaaa cggtttatgt cgctctgccg cctggcgcaa caggtgattt
    42241 gccagtagcg gaatgtcttc tgtgcgctcc gccagtgccg gaattttcag gctgacaacg
    42301 ttgaggcggt aatagagatc ttcgcggaat tccccgcgcg ccatcgcttt tggcagatca
    42361 cggtgagtgg cagaaataat ccgcacattg atatcaatat cgcggttact gcccagcggg
    42421 cgcactttac gctcctgcaa cacgcgcaac agtttgacct gcaacggcgc aggcatatcg
    42481 ccaatctcat cgagaaatag cgttccgcct tccgccgcct ggaataaacc ttcgcgattg
    42541 ctgacagcgc cagtaaacgc gccacgcgca tgaccaaaca gctccgactc cagcaattgc
    42601 tcaggcaatg caccacagtt aatagcaata aatggtttgc tattgcgcgg gctggcgttg
    42661 tggatagcct gggcgaaaat ctctttcccg gtgccgctct ggccattaat caaaacgctg
    42721 acgtctgatt gcgccaccag ccgcgcctgt tccagcaaac gcagcatcag cgggctgcgg
    42781 gtgacaatcg cctcgcgcca gcgttcgtcg gtggctggcg cggattgttc cagcgcatca
    42841 tcaattgcct gatatagcgc gtctttatca acaggcttgg tgaggaaact aaaaacgccc
    42901 tgctgtgttg cagcaacggc atcgggaata gaaccatgcg cggtaagaat aattaccggc
    42961 attcccggct gcactttctg gatttcagca aacagctgca taccgtccat ttcatccatc
    43021 cgcaggtcgc tgatgactaa atctactttt tcgcgattca gtacccgtaa tccttcagcg
    43081 ccactttccg ccgtgaccac gctgtagcct tcgctggtca ggcgcaggcc aagcagtttc
    43141 agcaatcccg gatcgtcatc gaccaataat aaatgcgcag gtttatggct catcaggagt
    43201 gacctcatgg gtggatggcg cgggcttttc actctcgtgt ggcgtatccg gcgagaaatt
    43261 tccagccggt ttgcgggtcg agagctggcg ttcaatatca gtcaggtttt ccagcttgcg
    43321 ggtggtgagt tccagttgct gctgtagaac gtgatgttgc tggcgcaatg tatccagctc
    43381 gctgtcgctg gactgctgga gtttactgta gcgttggcgc tcttccgcca gttgcagttg
    43441 cagcgcctga ccgtcgcgcc agagttgata cagtgggcga acctgtgccg ggatctcggt
    43501 acttaacgct tcaatacgcg cgaccagctg gcggcgctca tatggcgtaa ttttggcgtc
    43561 ggcgagcaaa atcccttgtt taaaggtatt ttgccagctg ccgtcgtcat attggcgagc
    43621 ttgctgacgc gactgcgcag gcattaaacg atcagcacaa tccatcgccc gcagccagta
    43681 aagcggattg gtttcggttg atttaccttg cagcgcccag atgtcgctac attcagtaga
    43741 aagatagtca gccagttgat aaaccggaat tttttcttct gctggcgtat caatggctgg
    43801 cttattgtga ttctgcacgc aacctagcaa tgccagacat ggcagccctg ccagccacag
    43861 ccgtcggggc aataatcgtt gaaaaatgtg tcgcatattc accagactta aagcctatcc
    43921 cagtgggcgt aattgttgca gacagtttgg acatggacag cgcggagaaa ccggagcgta
    43981 catgtcgtac gtgaggattt cgagcactgc ccggggccga actgacaaat aaaatagcct
    44041 gatgggatag gctgttagca ttatttcgtg tttttcgacg acggtaattc aatgcggaaa
    44101 caaacgtctt gcccgctctc gtcgaccaga tacagttccc cttgcatacg gcgaatacaa
    44161 tccctggcaa tgcttaatcc cagaccgctg cccttcaccg ccccttttcg ctggtggctt
    44221 ccctgaaaaa agggttcgaa gatcatggcg cgttcctctt gcggaatggg cgtgcctgta
    44281 ttgatgacat caatataaac ccgcgcacca tgtaaactgc tgcgaaggca aatgttaccg
    44341 gattcagccc cgtagtgcac cgcattggag taaagattat ccagtacgct catcagcagc
    44401 attggctccg ccaggcaagc tgttgctttg agatcgacgt cggtatgcat cattttagcc
    44461 cgtgcgggca ggctatgagc agaaactact gtctccacca gcggtgctaa ctcaacatta
    44521 tctagttcca ccgcactgtc cgcctgttta cggttgtaat caagcagttg ttcgatcagt
    44581 ttttgcaaat tgcggctgct gctatcaaga atgctcacca cctctttttg ctctggcgta
    44641 agcggcccga caacctgatc agccagtaat tcagtgccct cgcgcatact cgccagtggc
    44701 gtttttaatt catgagataa atgtcttaaa aattgatggc gttgggattc cagccatgac
    44761 aggcgctcac ttaaccaaag aatacgttgc ccaaccgagc gtaactcgct cggtccactg
    44821 aacgaaacgc tattgcccag agaacgccct tcccccaacc gattgatcat ccgttcaata
    44881 tttttcaccg gcccgataat catccgcgtg aaaagcagta ccattaccag actcaccaga
    44941 aatagcacca gcgattgcca accaaaatat tgcccacgtt cggcgatttc acgctgaagt
    45001 tgctgcccac gagagaacac tactgtgcgc gtggcctgta ccatttcggt attggcactg
    45061 gcaaaggctt ccagacgcgc ggcagcagca gcatcgggac cactgttgtt gcactgaagt
    45121 tgggccagat tgttcaagtc ctgacgcaat gcctggtaaa gtttatcgtc tggcagtacg
    45181 cctgcgtggg catcgagcat ttcgctgtaa cgcttgcgct ggctttgata aaccttcgca
    45241 agcgtagggt catccagcac gcaatactga cggtagctac gctccatctc cagcgccgcg
    45301 ttggtcattg cttcgctgcg ccgggcatcg ataagcgtgg tgcggttgac cagcgccgcc
    45361 tggtcgctaa gcgcattcag gctttgccag gcttgccatg ccagcaccaa caggggcagc
    45421 agaatcagca aaaatgccag cattaccagt tgtcgtaatg agcggggaaa aacgggccag
    45481 cgtttcaagg tgttactctc gtcagacgcg aatagcctga tgctaaccga ggggaagttc
    45541 agatacaaca aagccgggaa ttacccggct ttgttatgga ataaggcggt gcctaactcg
    45601 acgtttcgcc cgatggttga tatagctacg ctgatatcag aagttggacg gcaggcacct
    45661 tgttgtgcgt cattcgaatt ttatgtagca cgtcccgaag gggctgacat aagtcggtga
    45721 atgagccact gattactatt atgcagtaac tgtgccaata aagaaaatag tttggtaacg
    45781 tactgattat acgttgcttt gaggggttta tggcttcccc gctatttgaa tgtaaatcag
    45841 ccactgtgtc gctaaaaaga gacaacttaa gaaaaactta ttagattaca ataaaatcaa
    45901 gcatttaagt gtcgccagaa agcgacacgg taaaccaccc attgtcgtga tttacagaca
    45961 caaaaaagct cccgaagttg ggagcttata atagcggttg tatttaatgc cggatgcggc
    46021 gtgaatgcct catccgggct gcaaaaccaa tgagtttgac ggcttacccc aactgcttac
    46081 gtgcattgcg gaaaatgcgc atccatgggc catcctcgcc ccagttttca ggatgccagg
    46141 agttgctgac agtacggaaa acacgttccg ggtgcggcat cataatggtg actcgaccgc
    46201 tttcagtcgt gaccgccgta ataccgttcg gagaaccgtt cgggttagcc gggtaggttt
    46261 cagtgacttt gccgaagtta tcgacatagc gcagtgccac cagccctttg ctttccagtg
    46321 ccgccagatg cgccgcatca cgcacttcca cgcgcccttc accatgagag acagcaatcg
    46381 gcatttgcga gcccaccatc ccctgcaaca gcagcgacgg gctttgggtc acttcaacca
    46441 agctgaaacg cgcttcaaag cgatcggagg tattgcgcac aaaacgtggc cacaactcac
    46501 tacctgggat cagttcacgc agattagaca tcatctggca accgttacat acccccagcg
    46561 ccagcgtttg cggacggtgg aagaaggttg caaactcatc gcgtacacgg tcattgaaca
    46621 ggatagactt cgcccaacct tcaccggcac ccagcacatc accgtaggag aaaccaccgc
    46681 acgcgaccag ggcgtggaaa tcctccaggc ccgtgcgtcc agccagcagg tcgctcatat
    46741 gcacgtcgat agcatcgaag cctgcacggt ggaaagctgc cgccatttca acatgcgagt
    46801 tcacgccctg ctcacgcagc acagcaactt tcggacgtgc gccagtggca atatacggtg
    46861 ctgccacatc ttcgttgata tcaaacgaca gtttcacatt caggcccgga tcggcgtcgt
    46921 ttgatttcgc ctgatgctcc tgatcggcac actccgggtt gtcacgcagg cgctgcatct
    46981 gccaggtagt ttctgcccac cagacacgca acgtagtgcg gctttcgctg aatacagtct
    47041 gcccgttggc ggtaatcaca aaacggtcac cggaaaccgc ctgcccaaca taatggacgc
    47101 aatcagcaag cccatgctgt gccagtacgg actcgaccgc ttcacggtca gcggcacgaa
    47161 cctgaatgac cgcacccagt tcttcgttaa acaacgccgc caggcgatcg tcacccagag
    47221 tggcgatatc cgcgtcaatg ccgcaatgac cagcaaaggc catttccgcc agcgttacca
    47281 gcagaccgcc atcagagcgg tcgtgatacg ccagcaactt acgctgtgca accagcgcct
    47341 gaatcgcgtc atagaagcct ttcagttgcg cgacatcgcg tacatctgcc ggtttgtcgc
    47401 caagctgacg ataaacctgc gccagcgccg ttgcgcccag cgcgttattg cctttgccca
    47461 aatcaatcag cagcagtgcg ttatcttcgg tagaaagctg cggcgtgatg gtgtgacgta
    47521 catcttccac gcgggcaaat gcagaaatca ccagcgacag cggcgacgtc atttcgcgct
    47581 cttcgttacc ttcctgccag cgggttttca tcgacataga gtctttaccc accgggatcg
    47641 tcaggcccag cgccggacaa agctcttcgc ccacggcttt aacggcttca tacaggcccg
    47701 catcttcacc agggtggcct gccgccgcca tccagttggc ggaaagttta atgcgtttga
    47761 tatcgccaat ctgtgttgcg gcaatattgg ttaacgcttc accgaccgcc agacgggcag
    47821 aggcggcgaa atccagcagc gcaaccggcg cacgctcgcc aatcgccatc gcttcaccgt
    47881 agtagctgtc gaggctggcg gtagtgaccg cgcagttagc gaccggcacc tgccacggcc
    47941 ccaccatctg atcgcgcgcc accatgccgg ttacgctgcg gtcaccgatg gtcaccagga
    48001 aagttttctc cgccacggtc ggcagatgca gtacgcgttt caccgcgtct gcaatggtga
    48061 tcccttcacg gaccagcgca tcgcccttcg ctttcagcgt ttgtacatcg cgggtcatct
    48121 tcggcgtttt accaagcagg acgtccagcg gcagatcgat cggctgatta tcaaaatgac
    48181 gatcgtgcag agaaagatgc agttcttcgg tcgcttcacc aatcaccgcg tagggtgcgc
    48241 gctcacgctt acacagttcg tcaaacagcg gtaattgatc ggcagcaacc gccagcacgt
    48301 agcgttcctg ggattcgtta caccagattt ccagcgggct catgcccggt tcgtcgctta
    48361 gaatatcgcg cagttcaaat ttaccgccgc gcccgccgtc gctcaccagt tccggcatcg
    48421 cgttagaaag accgccagcg ccgacgtcgt ggataaacag gattgggttg gcatcaccaa
    48481 gctgccagca acggtcgatc acttcctggc agcgacgctc catctccggg ttgtcgcgct
    48541 gtacggaagc aaagtcgagg tcggcatcag actgacctga cgccatggaa gacgccgctc
    48601 cgccgccaag accgatgttc attgccgggc cgccgagaac gaccagtttc gcaccgacgt
    48661 tgatctcgcc tttttgtacg tgatcggcgc gaatgttgcc gatcccgccc gccagcatga
    48721 tcggtttgtg ataaccgcgc agctcttcgc cgttgtggct gttcactttt tcttcataag
    48781 tacggaagta gccattcagt gccggacgac caaattcgtt gttaaacgcc gcgccgccca
    48841 gcgggccttc ggtcatgatg tccagcgcgg tgacaatgcg ctcaggctta ccgaaatctt
    48901 cttcccacgg ctgttcgaag ccaggaattc gcaggttgga aacggagaaa ccaaccaaac
    48961 ccgctttcgg cttcgcgccg cgcccggtgg caccttcatc gcggatttca ccgccggaac
    49021 cggtcgccgc gcccggccac ggagaaatcg ccgtcgggtg gttgtgagtt tcgactttca
    49081 tcagaatatg cgccggttcc tgatggaaat cgtagcggcc cgtttcgtgg tcagcaaagt
    49141 agcggcccac ttcagaacct tccattacgg cggcgttatc tttataagca gagagaacgt
    49201 gatctggcgt ggtttcgaaa gtatttttga tcatcttgaa cagcgatttc ggctgctgtt
    49261 caccgtcgat aatccagtcg gcattaaaaa ttttgtggcg gcagtgctca gagttcgcct
    49321 gagcgaacat atacagctcg atgtcgttcg ggttacgacc aagctttgtg aaagcatcct
    49381 gcagatagtc aatttcatct tccgccagag ccaggccaag acgcaggtta gcgtcgatca
    49441 gcgcctgacg gccctgcccc agcaaatcaa cgctggtaac cggagtcggt tgatggtggg
    49501 caaacaactg ctctgcatca tctaaagcaa aaaagaccgt ttccatcatg cggtcgtgca
    49561 gttcagcggt aacctgctgc cattgttcat tggtcagcgt accggcttct atatagtaag
    49621 caacgccgcg ctcaaggcgg tttacctgtt gtagcccgca gttatgagca atatcggtcg
    49681 ctttcgaaga ccagggagag atggtgccag gacgcggggt caccagcagg agtttgcctt
    49741 gcggggcgtg gctggcgagt gccgggccat atttcagcag gcgttcaagt tgtgcgtgct
    49801 catcatcgtt taacggcgca ttgaggtcag caaaatggac atactcggcg taaatattgt
    49861 gaaccgggag cctggcagcc tgaaaacgtg ccagcagttt gttgattcgg aatgccgaca
    49921 gtgcaggcga accacgcaga atttccatca taagtctctc gtcttctaag ctttcggtgt
    49981 acccaagggg ggaaacgggc gtcattataa agaatctgat gcgctgacga aaccgtttgc
    50041 gtggaaataa aatcaccatc gtgaattagc aacgcgtgcc gccaatggct gtaataagtt
    50101 gccatctggc gcaggtttac gcaaaatgcc gctcatttat gagtaaacct ttcactatta
    50161 ttacattttt tcaagctggg acgcgcacga cacagagaat taactaattg aaaaaattaa
    50221 agattaatta tctgttcatc ggcattctgg cactgctgct cgcggtcgct ctctggccat
    50281 ccattccctg gtttggtaaa gccgacaacc gtatcgccgc cattcaagcg cggggagagt
    50341 tgcgtgtgag caccattcat actcccctga cgtataacga aatcaacggg aaaccttttg
    50401 gcctggatta cgaactggcg aaacagtttg ccgattacct cggcgtaaaa ctgaaagtga
    50461 ccgtgcggca gaatatcagc cagctgtttg acgacctcga taatggtaac gccgacctgc
    50521 tggcggcagg acttgtctat aacagtgagc gggtaaaaaa ttatcagcct ggccctacct
    50581 attattccgt gtcacaacaa ctggtttata aagtgggtca gtatcgccca cgtacgctgg
    50641 gcaacctgac ggcggagcaa ctcaccgttg caccgggtca tgtggtggtt aacgatctcc
    50701 agaccctgaa agaaacaaaa ttcccggaat taagctggaa ggtagacgac aaaaaaggct
    50761 ctgcggaatt aatggaagat gtcatcgaag gaaaactcga ttacaccatt gctgattctg
    50821 tcgccatcag cctgtttcag cgcgttcacc cggagctcgc cgtagcgctc gatatcaccg
    50881 atgaacaacc ggtgacctgg tttagcccgt tagatggcga taataccctt tccgccgccc
    50941 tgctcgactt cttcaacgaa atgaatgaag acggtacgct ggcacgcatt gaagagaaat
    51001 acctggggca tggcgatgat tttgattacg tcgatacgcg cacattttta cgcgccgtcg
    51061 atgcggtact gccgcagtta aagcccctgt ttgagaaata cgccgaagaa attgactggc
    51121 gtttgctggc cgctattgct tatcaggaat cgcactggga tgcacaggcc acttcaccga
    51181 cgggtgtgcg cggcatgatg atgttaacca aaaataccgc gcaaagcctc ggcattacgg
    51241 atcgtaccga tgccgaacag agcatcagcg gtggcgtgcg ttatttgcag gatatgatga
    51301 gtaaagtgcc ggaaagtgtg ccggagaacg agcggatctg gtttgccctc gctgcgtaca
    51361 atatgggcta tgcgcatatg caggatgccc gcgccctgac ggcaaaaacc aaagggaatc
    51421 ctgacagttg ggctgacgta aaacagcgtc tgcctttact tagccagaaa ccctattaca
    51481 gcaagctgac ttacggctac gctcgtgggc atgaagccta cgcttatgtc gaaaatattc
    51541 gtaagtatca gattagcctg gtgggttatc tgcaagagaa agagaagcag gctacagaag
    51601 cggcgatgca actggcgcag gattatccgg cggtatcgcc tacggagttg ggcaaagaga
    51661 aatttccttt tctctcgttt ctttcccagt cgtcatcaaa ctatttgacc cattctccct
    51721 ctctgctgtt ttccaggaaa gggagtgaag agaaacaaaa ttaatccgtc gaggattgcg
    51781 cttttttctg cgctttaatt tcctggcggc gcatgcgaaa gaagtcactg agcaacgccg
    51841 cgcactcatc cgccagtatt ccttccgtaa tttccactcg gtgattcata cccggatgat
    51901 gcagcacatc cattaaagat cccgcagcgc cagttttcgc gtcacgcgca ccaaagacca
    51961 cgcgaccaat gcgactgtgg atcatcgctc cggcacacat tacacatggt tcaagcgtga
    52021 catacaacgt ggcgtcgatc agacgataat tttgcatcac cagaccgccc tgcctcaggg
    52081 ccatgatttc tgcatgtgcg gtgggatcat gtcgaccaat cggacggttc cagccttcgc
    52141 caataacacg attgttatgc actaataccg cgccgaccgg tacttcccgc tcatcccagg
    52201 cacgcttcgc cagcgtcatt gcatgacgca tccagtattc gtggctaaat tcgacttcag
    52261 acaaaaagaa aactccggtt ataaaagcgc ggcgcattat acacggacgc tatgctttac
    52321 tctattccag ttgctggagt tcaccgcgcg gggttactcg ccagcgatgc tggcaaaagt
    52381 aaagcagcgg gttgtcctga ttactgtcgc tatagccact gtacagccgc agcggcgtgc
    52441 cgattttgcg ctccagttgc gcgacctttt catgcccgag gcaacgcatc gtcaataccc
    52501 aaccaccata gccacgctga atttggctgg cgataagatt cacccgcggc agccagggtg
    52561 tatcgaaata gaccgcttca accagcggct gtggagaacc ggtaatcaac cagatatcag
    52621 catcggaact taacaggtag gtggttaatc gctcctgaac cagcggaaag gcggtaacat
    52681 tgtcacgaaa ccagcgtacg aaatcggcct gcaacgcctg taaacgtgct tcgctgtgac
    52741 caaaggtgca tccccacaga agcagactca tcggccagcg tgccgcacga ccttttatca
    52801 ataacgcaat ggctataatc ggcaacaacg gcaggacaag taacgcattc agcggttggc
    52861 gacgcagtaa atagcgcaga aaactgccga acatatcctg ctgatgcaat gttccatcta
    52921 agtcaaaaaa caccacacga cgctcgtgag ttgccaaacc atactcctct ggactgaaac
    52981 atcctgatta attttcgcta ctacatagcc taacagatag atcatcactt ttccggcaac
    53041 aatcgaccgc atcatggcta actggaattt ttaattcatg gcaattagcg gcaatggaat
    53101 ataaaattca ctcgcgtgtg tctcatattg cctgttgtcg ccactatttc atcgcgagga
    53161 aagatatgaa cggcttactt cgtatccgtc agcgttacca ggggcttgcc cagagtgata
    53221 aaaaactggc ggattatctg ctgctacaac ctgatacggc gcgccattta agctctcagc
    53281 aactggccaa cgaagccgga gtcagtcagt ccagcgtcgt gaagttcgcg caaaaactcg
    53341 gctataaagg ttttccggcg cttaagttgg cgttgagtga agcgctggca agccagccgg
    53401 aatcgccctc agtgcccatt cataaccaaa tccgcggtga tgatccgtta cggctggtcg
    53461 gcgaaaaact gattaaagaa aatactgccg ctatgtacgc aacgctaaac gttaatagtg
    53521 aagagaaact gcatgaatgc gtaacaatgt tgcgctctgc gcggcggata attctgaccg
    53581 gtattggcgc ttcgggtctg gtggcgcaaa actttgcctg gaagctgatg aagattggct
    53641 tcaatgctgc cgcagtgcgc gatatgcatg cgctactcgc taccgtacag gcgtcgtccc
    53701 ctgacgatct gttattagcc atttcttaca ccggtgtacg acgcgagtta aacctggcgg
    53761 cagatgagat gctgcgagtg ggcggaaaag tgctggcgat taccggcttt actcccaatg
    53821 ccctgcaaca gcgtgcttct cattgcctgt ataccattgc cgaagaacaa gcgacaaaca
    53881 gtgcttcaat ctctgcttgt cacgcgcagg gaatgttaac ggatttgctg ttcattgcgc
    53941 tgattcagca ggatctggaa ctggcaccag aacgtattcg tcatagtgaa gcgctggtga
    54001 aaaaactggt ctgagtaaag aatgcgcgta taatgcccgc ccggtttgtg ttgttttgag
    54061 agtttcctta tggcgctgtt aatcactaaa aaatgcatca attgtgatat gtgtgaaccc
    54121 gaatgcccga atgaggcgat ttcaatggga gatcatatct atgagattaa cagcgataag
    54181 tgtaccgaat gcgtagggca ctacgagaca ccaacctgcc agaaggtgtg cccgatcccc
    54241 aatactattg tgaaagatcc gcaacatgtc gagacagaag aacagttgtg ggataaattt
    54301 gtgctgatgc accacgcgga taaaatttaa tattctactc tggaagtaga gtattaatta
    54361 ttttactggg aagccttaac gccattatat ttatttaatt gatgacatta gcataataat
    54421 tcactaagtt aatttatata gtatctgccc agacacttat ttatagttat taaaggcgcg
    54481 tccgattggt tcaccggacg caccttaagt acgtttcctt gtgttataag aacagaagga
    54541 tcagctgtaa aacagcaatg atgattttga tgacccgttt aatcaggtat cggcaatcag
    54601 tcattcgttt ttccttaaac aaggaatgca gccgttcaga ttagccctta catctcccca
    54661 aaactgaacg tgcgagttat tggaatcgag tgctgcactc cacaccagag ctttgacgac
    54721 accactcgtt tcaatggggg aattcagtgg catggtgtaa agcacagcaa aattgccaat
    54781 aacgaaaccg attttaatgt acttatcaaa aagcggtcac gttaatcttt tcatcaatgt
    54841 cgatttcttc gtaaaggctc gcgtttttta tgtcagagat tgcaagttgc ttgtacaggt
    54901 caacgaaatt gatccataat cattttcgtt ggaatcgtgt gctgcacaaa attaaagtta
    54961 aaaagtaaaa cccccgttcc ttaccagttc gggggtttta ctttataaag agaacggtat
    55021 tatttttaac tttcaataat taccgtggca caagcatagt ggcgctcatc ggccagcgtc
    55081 acatgcatat ttgcaacgcc cagcttttcc gccagtttta atgcctcgcc ccacagtcgc
    55141 agccgtggct taccaagctc atcgttaaat acttcaaact gattaaacgc cagaccattg
    55201 cggatcccgg tgccaaacgc ttttgctgcg gcttctttta cggcaaagcg tttcgccaga
    55261 aaacgcaccg gctggtggtg cgttttccaa atttcccatt cgttatcgct taatacgcgg
    55321 cgtgccaggc gctcaccgga tcgggcgatc actgcttcta tgcgagcgat ctctacgata
    55381 tccgtgccta aacctaatat tgccattagc cacgcgcttc cagcatcagg cgcttcattt
    55441 ctgccaccgc atctttcagt ccggtcatca ctgcacgacc aataatggca tgaccgatat
    55501 tcagttcgtg catctcaggg atggcggcaa tggcttttac gttgtgatag gtcagaccgt
    55561 gtccggcgtt aactttcaaa ccgagactag cggcaaaggt cgcggctttg gcgatacgcg
    55621 ccagctcttg cgcctgttcg gcgtcagttt tggcatcagc atagcaaccg gtgtggatct
    55681 cgataaacgg tgcgccaacc tctgccgcag ctttgatctg ctcttcatcg gcgtcgataa
    55741 acagagaaac ctgaatcccg gcatccgcca gacgtttgca ggcatcgcgc attttgtcac
    55801 gctgccctgc aacgtccagg ccgccttcgg ttgttacttc ctgacgcttt tctggtacca
    55861 ggcagcaaaa atgcggcttc gtctcaaccg cgatcgccag catctcttcg gtcaccgcca
    55921 tctccagatt catgcgggta tccagcgtct gacgcaggat gcgcacatcg cggtcagtaa
    55981 tgtggcggcg atcttcacgt aaatgcacgg taatgccgtc cgcccccgcc tgctcggcaa
    56041 taaacgcggc ctgcaccgga tccgggtaag cggtaccgcg cgcgttgcgc agcgtagcga
    56101 tatggtcaat gttgacgcct aacagtaatt cagccatgac aatcctcatc attcataatg
    56161 tgttttcacc gttcgcttag gcataaattg ccggaacagt tccctgctct ttaaaggttt
    56221 accgccaaga tacggcttaa gcgccatgcg ggtaaagcgt ttcgcggcgc gcagtgtgtc
    56281 tgcgtcagga aattcccgtg cgtttaacgc ttttaactgc cttccggtga acgttttatt
    56341 gtcgataacg acgcttgcga taaacccttt ttcttcgcga taacgatacg tcatggtgtc
    56401 atctaccggc tcgccgctac ccgcacaatg ggtaaaattg acgccataac ccagatgccc
    56461 gagcagtgcc agttcaaagc ggcgcagcgc gggttctggc gtaccagtga cccctgcaag
    56521 agactgaatg cagtgcaagt aatcgaaaaa gagttcagag aagcgcgtct cgtattccag
    56581 tacgcgggag agaagttcgt tgatgtacag accgctgtaa agcgtgatac cgcttaatgg
    56641 cagcgccagc gagacagctt cagcactgcg cagcgttttg acttcgccac gcccgccaaa
    56701 acgtagcaag agaggggtga aaggctgtaa tgcacctttc agggtagagc gtttagagcg
    56761 tgcgcctttg gcaaccagac gcacgcgacc cgattcctcc gtgaagacgt ccagcatcag
    56821 gctggtttcg ctccacgggc gactatgcag gacaaatgcg cgctgccagc cttccatcgg
    56881 agttactctt aaagatcgtc aacgtaaccg agactgcgca gtgcgcgttc gtcgtcggcc
    56941 caaccggatt tcacttttac ccacagctca aggtgaacag gcgcttcaaa catttcctgc
    57001 atgtctttac gcgcttcaat cccgatggtt ttgatcttgg cccctttgtt gccaatgacc
    57061 atcttcttct gcccttcacg ctcaacgaga atcaaaccgt tgatgtcata accaccgcgt
    57121 tcgttagaga cgaaacgttc gatctccacg gtcacggagt acggcagttc agcgccgagg
    57181 aaacgcatca gtttttcgcg gatgatttca gacgccataa aacgctgtga gcgatcggtg
    57241 atgtaatctt ccgggaagtg atgagtcgct tcaggtagat gcttacgcac gattgccgca
    57301 atagtggcaa cattcagccc ggtttcggca gagattggca cgatatcgag gaagttcatc
    57361 tggcttgcca ggaactgcag gtgcggcagc agatcggctt tctcctgcac gttgtccact
    57421 ttgttcaccg cgaggattac cggcgctttg ccttcgcgca gtttgttgag caccatttcg
    57481 tcgtccggcg tccagcgggt gccttcaacg acaaaaatca ccagctcaac atcgccaata
    57541 gagctgctcg ccgctttgtt catcaggcgg ttaatagcgc gtttttcttc catatgcagg
    57601 cccggtgtat cgacgtagat cgcctgatac gcgccttcag tatggatccc cacaatgcgg
    57661 tgacgagttg tctgcgcctt gcgggaggtg atggagattt tctgccccag cagtttgttc
    57721 aacaatgtgg atttgccaac attcggacgt ccgacgatgg caataaatcc gcagtaactt
    57781 ttatcgatgc tcattccagc tccagttttt tcaacgcctg ttcggcggca gcctgctcag
    57841 ccttacgacg gcttgaacct gtgccaacca ccggttcact caggccgctg acctggcagt
    57901 ggattgtaaa ttcctgatcg tgcgcttcgc cacggacctg aactaccaga taagtcggca
    57961 gcggcagatg acgaccctgc aaatattctt gcaagcgcgt tttcggatct ttttgtttat
    58021 cgcctgggct aatttcgtcc agacgagttt gataccagtt gaggattaat ttctcgacgg
    58081 tttgaatatc actgtcgagg aatacgccac caattaatgc ttcgacggtg tcggcgagaa
    58141 ttgactcacg acgaaatcca ccgcttttaa gttcacctgg tcctaaacgt aagcactcgc
    58201 ctaactcaaa ttcgcgcgcc agttccgcca gcgtattgcc acggaccagc gtggcgcgca
    58261 tccggctcat atcgccttca tccacacgag ggaaacggtg ataaagcgca ttggcgataa
    58321 cgtagctcag aatagagtcg cctaaaaatt ctaaacgctc gttatgtttg ctgctggcac
    58381 tacgatgagt taatgcctgc tgcaacagtt cctgatgatt aaaagtgtag cccagcttcc
    58441 gttgaagccg attaattacg atggggttca tgcgatacca ataaataaat gcgtcaacaa
    58501 ttcagcacac gaaacagacc tgatatacat ggctctgcta actgcttcgc tgcagtttca
    58561 gtttagcggt atatggggac caacgctgtt tcgtgtgccg tggcaacctg gaggtgccaa
    58621 ccttaaactt cgggggaata ttctatacac aacgacgggg gatgtcgtta gccacgggag
    58681 atttatctca taaataattc acgttgtcgc cataacggcg acaacgtgaa cgaagatgag
    58741 ctattaatgg atgccgccaa tgcgacttaa gcgcacacct gtcggccatt cgccttcttg
    58801 cttatcgaag ctcatccaga tagccgttgc ccgaccgacc agattcgctt caggcacaaa
    58861 gccccagtaa cggctgtccg cgctgttgtc gcggttgtcg cccatcatga agtattgtcc
    58921 cggaggaaca atccaggttg ccagttgttg ccctggctgc tggtaataca tccccacctg
    58981 atcctgcgca atcggcactg tcagaatgcg gtgcgtcaca tcacccagtg tctctttacg
    59041 ctcggaaaga cgaattccat tttctttggt ttcgtttttc ggcacttcaa agaatccgct
    59101 ggtcgcttcc ccaccattac ggcgtgagaa ggtctgaacg aaatcgctcg gttccacgtt
    59161 tgagtaggtg accggcagcg cgttttcaca cgcctggccg gaactgcatc ccggttgaat
    59221 cgtcagctct tttgagaccg gatcgtaagt gactttatcg cccggtaaac ccaccgcgcg
    59281 cttgatataa tcaagctttg gatcttccgg atatttaaag accacgatat cgccgcgttt
    59341 cggatgaccg gtttcgatca gcgttttctg gtagatagga tctttaatgc cataagcaaa
    59401 cttatctacc agaataaaat caccgattaa cagagtcggc atcatcgaac ctgacggaat
    59461 ctggaacggt tcataaataa acgaacgcac aatcaatacg atagccagta ccggaaaaac
    59521 agaagcaccg gtttccagcc agccaggctt cggcgcaacc tttttcaacg ttgctttatc
    59581 cagtgagtca ccggcagccg cctgcgccgc tgcctgacgt tcccgccgtt taggtgcgaa
    59641 aaagaattta tccacgcacc ataaaatgcc cgtcaccagt gtggcaatca ccagaatcag
    59701 ggcaaacata ttcgccatgc caactcctaa gggttatttg ttgtctttgc cgacgtgcag
    59761 aatggcgagg aacgcttcct gcggcagctc gacgttaccg atctgcttca tgcgtttctt
    59821 accttctttc tgcttctgca gcagcttttt cttacggctg atatcgccgc cataacattt
    59881 agccagtacg tttttacgca gctgtttcac ggtggatcgc gcaataatgt gcgtaccaat
    59941 cgctgcctga atggcgatat caaactgctg gcgtgggatc agatctttca tcttctccac
    60001 caactcgcga ccgcggtttt gcgaattatc acggtgggtg atcaacgcca gcgcatcaac
    60061 acgttcaccg ttgattaata cgtctacacg taccatgtcg gacgcctgga agcgtttgaa
    60121 gttgtaatcc agagacgcat aaccacgcga ggtagatttc aggcgatcga agaagtcgag
    60181 caccacttcc gccatcggga tctcgtacgt cagcgccacc tgattaccgt ggtaaaccat
    60241 attggtctgc acgccgcgtt tttctacgca cagcgtaata acgttgccga gatatgcctg
    60301 cggcagcagc atgtgacact ctgcaatcgg ctcgcgcagt tcgtaaatgt tatttaccgc
    60361 aggcagcttg gatgggctgt cgacgtagat aacttccctt gacgtggttt caacttcata
    60421 cactacggtc ggcgcagtgg tgatcagatc cagatcgtat tcacgttcca gacgctcctg
    60481 gatgatctcc atgtgcagca ggccaaggaa gccgcagcgg aaaccaaagc ccagcgcgct
    60541 ggagctttcc ggctcataga acagtgaggc atcgttcagg ctgagtttac ccagcgcgtc
    60601 acggaaggct tcatagtcgt cggaacttac cgggaacaga ccggcgtata cctgcggttt
    60661 gactttctta aagccaggca gcgccttttc tgccggatta cgcgccagcg ttaaggtatc
    60721 gccgactgga gcgccgtgga tatctttaat cgcacatacg agccagccta cttcgccaca
    60781 tttcagttca gtgcggtcaa cctgtttcgg cgtgaagatg cccagacggt cggcgttata
    60841 ggtctgcccg gtactcatga ctttcacttt gtcgccctta cgcagggtgc cgtttttaat
    60901 acggataagt gaaacaacgc ccaggtagtt gtcgaaccat gagtcgataa ttagtgcctg
    60961 caacgggcct tccggatcgc cttccggcgg cggaatgtcg cgcaccagac gttcgagaac
    61021 gtcctgcacg ccaacgccgg ttttcgctga acagcgcacc gcgtcagtgg cgtcgatgcc
    61081 gacgatatct tcaatttctt ccgccacgcg ttcaggatcg gctgccggca ggtcaatctt
    61141 gttcagtacc ggcaccactt cgagatccat ttccatggcg gtgtagcagt ttgccagggt
    61201 ttgcgcttcc acgccctgcc cggcgtcgac caccagcaat gcaccttcac aggcagccag
    61261 cgaacgggaa acttcatagg agaagtctac gtggcccggg gtgtcgataa agttaagctg
    61321 ataggtttcg ccgtcagacg ctttgtagtc cagcgtcacg ctttgcgctt tgatggtaat
    61381 gccacgctca cgctcaagat ccatggaatc gagaacctgc gcctccattt cacggtcaga
    61441 caggccaccg cagatctgga taatacggtc agacagcgtc gatttaccgt ggtcaatgtg
    61501 agctatgatc gaaaagttac gtatattctt cataaagtat gattattgtg ccttaatgcc
    61561 cggttaacca ggcttttaga agtcgctgtt ctgagcttaa cgtctgtatt aatagaaacg
    61621 ccgcattcta cactacaaca ttgaggcgag gaaatgttca taccgtatgg attgtggtat
    61681 ctggaaacgt cctcgcattt gttatgcaaa atgcaacaaa gccagtgaaa tcactggctc
    61741 gcgtcttccg aagatgtttc aaatcgcaca aggccaggcg gcaatgccac gcttaagatg
    61801 atcggttgcc attctgcccg ggcagcaaac ttacgcgagt agccacgcgc aatcaggaac
    61861 ccgccaatac cgccgaggac cgcaccacat aatgctgcga cgtcagaagc aaagagtagc
    61921 tgaaatagcg aagcgatgag gaataatccc accagcggcg acatataaac cagtaatgcg
    61981 gagctaagca ggctgccttc ggcgatcccc aattccactt tttgccccgg cactaacggc
    62041 tcatcacagg gtacgacaat ggtatgcgtg gtttgcgggc caagtttatt caacacgcgg
    62101 ctaccgcaac cggcgcgtga agcgcagctg ctgcatgagg ctttaacatc acaactgacc
    62161 agcgcctgcc cgttttgcca ggagacgacg gtagcccact ctttgatcat tgcgctgccc
    62221 cgaacttaat attctcggca atgcgtttcg ccgtttgcgg cggcagttca ccgacaatgg
    62281 tgatttcggc gttatcacgt acgcttgtac tgacggttct gcgtccggtg cgcaacatct
    62341 gatcggtgct cgatggcgta gcgcggttaa cgtttaccga gaagctgaat aatccgtcgg
    62401 aatagagacg tgattcgata ggcatgttgt ccatcgtcgg tagcggacgt cgactactgg
    62461 aaacttcgct aaaaccctgt ggcaaccagg ttggcgtcca gctgaattta gctttttcac
    62521 ctacaggaac agaaagcaac ggcggcaaat ttgccttcgc cagcgtctgc atactgctgc
    62581 tgatatcctg attgacgtta aaagcaatca cgcgaaattg ttccagcgtt tcaccatcgc
    62641 gatcaagaag atcaacccgc atcggtaatt tcgattcggt gtccatccac acgatgtagc
    62701 tgtagcgtgt accatctcgg gcaaccacgc gaatgacttc gcaaagacga tcagcaatac
    62761 gcgtgcgccc gacggagata aagtcgtagt aaggagaaag gcgtttgaaa tcggtataga
    62821 tgagcgatgg cagagaatca acaatgtaat cgccattaag cgtgaacggt tcaagtcccg
    62881 gttcaaaata gctgatttca ttgccgcgct gtaccacttc ccggcgcggg ccatccattt
    62941 gcaacaattg tgcaagagga cggttatcga ggcgtgcatg tcgataacgc agagactcaa
    63001 caccctgttt attgatgctg atgaatgaca gctcgtaatt cagtgactga ctggccaggt
    63061 tcatctgctg taataacgcc ccggacgcgg gagtggccga ggcgttagca gagaataaca
    63121 ggctacctgt cactaatgac atggcaaacc aaagttgctt cattactgcg attgcgttcc
    63181 taaagtttga attcctggca cctgtacagc ggcttgctgg gtttgcgcct gctcaaactg
    63241 aagctgttca gagtggagtc ggcgttgcag ttcgtaatcc tgcaacattg cattaatgcg
    63301 acgacgctgc tgctgttgac cattatttgc ggtcgcttca gaaggtactc ccaggctaac
    63361 cgggctggct ttacccatca tcggcagtgt attaaatacc ggcgtttcgg gctgctggga
    63421 cgtttcagat tgtccattat agtgctggac gccaacgata actgcaagcg atacgcatgc
    63481 ggctacgccc atttgggtaa gctgtgccgc ccacggacgt actttctgcc agaatggcat
    63541 tttctgccat tcatgcggcg caggctgggc ttccgggatc aatgtcgccg gttgacgtac
    63601 tggctcttct tcaatggcgg ccatcacgcg tgaagagata tcgaaatgga gcacctcggg
    63661 agtatcaccc cgcattgagt cacggattaa gtgatagctt tcccaggttt tctgcatttc
    63721 tgggttatga gccagttcgt taagcagctc actatccagc gtttcgccat ccattaaagc
    63781 ggaaagttgt tctttctgca tgcctaatac ccttatccag tatcccgcta tcgtcaacgc
    63841 ctgataagcg gttgaacttt gttatcaata gcttccctcg ctcggaagat acgtgaacgc
    63901 accgtaccta ccggacaatc catgatagcg gctatctctt catagctcag gccatccagc
    63961 tcccgcaagg ttattgccat gcgtaaatct tccgggaggg actcaatagt tcggaaaact
    64021 atctgtctca gttcttctga caacattaag ttctcagggt tcgaaatttc tttcaacgcg
    64081 ccgccacttt cgaagttttc agcttcaatg gcatccacat cactggaagg tggacgacgc
    64141 ccctgagcaa ccaggtaatt tttcgctgta tttacagcaa tccgatacag ccatgtataa
    64201 aaagcgctat ctccccggaa cgaatccagc gcacgatagg ctttaataaa agcttcttgt
    64261 accacatcgg gaacatcacc cgacggcaca tagcgggaaa ccagactcgc cactttatgc
    64321 tgatagcgca ctaccagtaa gttaaaggct ttctgatctc ccttctggac ccgttcaacc
    64381 aggacctggt ccgttaactg ctcgctcatc cgaggtaaag tctccccaaa ccaaatttcc
    64441 acgcgctatc gaaacgccac tccattagct gcaatttgag caagcaaagg gttagagtgt
    64501 ctcgtttttg taaagttccg taacgcatct gtttttgttt gtcatgctgt agacggatca
    64561 ttatctatca ttataagtct acagaatctg aacatcgcat tatctgtgta gaaatgccca
    64621 tttaactgcc tgaagagtaa cccaacggcc tttttatttc accacctaat cctccaccag
    64681 ccagtaactt ctctttttct cgccgccctg cgtcagcgtg tttagtcact gtaacaaata
    64741 ttaaaatagc aggtgtttat tcgcacaaca tgatgctatg ctgaccaaac cgtgtttagt
    64801 aaattaaaca aagaaaatga atactctcct tgaacattca tgtgacgtgt tgattatcgg
    64861 tagcggcgca gccgggcttt cactggcgct acgcctggct gaccagcatc aggtcatcgt
    64921 tctaagtaaa ggcccggtaa cggaaggttc aacattttat gcccagggcg gtattgccgc
    64981 cgtgtttgat gaaactgaca gcattgactc gcatgtggaa gacacattga ttgccggggc
    65041 tggtatttgc gatcgccatg cagttgaatt tgtcgccagc aatgcacgat cctgtgtgca
    65101 atggctaatc gaccaggggg tgttatttga tacccacatt cagccgaatg gcgaagaaag
    65161 ctaccatctg acccgtgaag gtggacatag tcaccgtcgt attcttcatg ccgccgacgc
    65221 aactggtaga gaagtagaaa ccacgctggt gagcaaggcg ctgaaccatc cgaatattcg
    65281 cgtgctggag cgcagcaacg cggttgatct gattatttct gacaaaattg gcctgccggg
    65341 cacgcgacgg gttgttggcg cgtgggtatg gaaccgaaat aaagaaaagg tggaaacctg
    65401 ccacgcaaaa gcggtggtgc tggcaaccgg cggtgcgtcg aaggtttatc agtacaccac
    65461 caatccggat atttcttctg gcgatggcat tgctatggcg tggcgcgcag gctgccgggt
    65521 tgccaatctc gaatttaatc agttccaccc taccgcgctg tatcacccac aggcacgcaa
    65581 tttcctgtta acggaagcac tgcgcggcga aggcgcttat ctcaagcgcc cggatggcac
    65641 gcgttttatg cccgattttg atgtgcgcgg cgaactggcc ccgcgcgata ttgtcgcccg
    65701 cgccattgac catgaaatga aacgcctcgg cgcagattgt atgttccttg atatcagcca
    65761 taagcccgcc gattttattc gccagcattt tccgatgatt tatgaaaagc tgctcgggct
    65821 ggggattgat ctcacacaag aaccggtacc gattgtgcct gctgcacatt atacctgcgg
    65881 tggtgtaatg gttgatgatc atgggcgtac ggacgtcgag ggcttgtatg ccattggcga
    65941 ggtgagttat accggcttac acggcgctaa ccgcatggcc tcgaattcat tgctggagtg
    66001 tctggtctat ggctggtcgg cggcggaaga tatcaccaga cgtatgcctt atgcccacga
    66061 catcagtacg ttaccgccgt gggatgaaag ccgcgttgag aaccctgacg aacgggtaat
    66121 aattcagcat aactggcacg agctacgtct gtttatgtgg gattacgttg gcattgtgcg
    66181 cacaacgaag cgcctggaac gcgccctgcg gcggataacc atgctccaac aagaaataga
    66241 cgaatattac gcccatttcc gcgtctcaaa taatttgctg gagctgcgta atctggtaca
    66301 ggttgccgag ttgattgttc gctgtgcaat gatgcgtaaa gagagtcggg ggttgcattt
    66361 cacgctggat tatccggaac tgctcaccca ttccggtccg tcgatccttt ctcccggcaa
    66421 tcattacata aacagataaa aagcctgggt tagcgccgta tacgcttcgg aatagttctg
    66481 gtctggccca cgaatgacta agcgatcgct aaagcattct cccgcctgcg gggagaatgc
    66541 cagcagcacc cgatgcggca gtcgcgcttc gttttccgcc acatccgtcc gcaaacgtaa
    66601 atgccagccc atacttaatg ccagctccgt aaaactatta ccaatctgct ctggcagcac
    66661 cacgcagaaa aatccctctt cggtaatgca ctccgccgcg caagtaagta aagtctggtg
    66721 atcaagtgag gtggtgtagc gcgcctgttc ccgctgcggc gtcgcgcact ccactccttg
    66781 ctcataatac ggcgggttgc tgatgattaa atcaaagcgc gcggtctgct gtgttaccca
    66841 ctgctgaata tccgccgtat ggacgttaat tctctctaac cacggggact gggcgacatt
    66901 ttcctgcgcc tgagtggcag cttcactttc cagctcaacg gcatcaatta tcacgctgtc
    66961 actggttcgc tgcgccagca tcagtgccag caagccacta cccgcaccga tatcaaggca
    67021 acgttttacc ccagccaccg gtgcccatgc gcccagcaat atgccatccg ttcccacttt
    67081 catcgcacag cgatcgtgag caacaaaaaa ctgtttaaaa gtaaatccat tacgacgaag
    67141 cacggatgta gactgtgaca tgaaaataaa accttgcagg aaaaacggcg atagcaccgg
    67201 gtgagaacaa tacccgagaa gcgatatcca tacaaacaga tgaagattgc agccgtaacg
    67261 tctataatca gcgccccaca cagaggtaga acatgactgt aacgactttt tccgaacttg
    67321 aactcgacga aagcctgctg gaagccctcc aggataaagg tttcactcgc ccgaccgcca
    67381 ttcaggctgc cgccattccg cctgcgctcg atggccgtga tgtactcggt tctgcgccga
    67441 caggcaccgg taaaacggcg gcgtatctgc tgccagcgtt gcagcacctg ctcgatttcc
    67501 cgcgtaagaa atccggtccg ccgcgtattt tgatcctcac cccaactcgc gagctggcga
    67561 tgcaggtggc cgatcatgcc cgcgaactgg cgaaacatac gcatctggat atcgccacca
    67621 tcaccggcgg cgtagcctat atgaaccacg cggaagtgtt cagcgaaaat caggacatcg
    67681 tggtcgccac gaccggacgt ctgctgcaat acataaaaga agagaacttc gattgccgcg
    67741 cggttgaaac gctgatcctc gacgaagcag accgtatgct ggatatgggc ttcgctcagg
    67801 atatcgaaca tattgctggc gaaacgcgct ggcgtaaaca gaccctgctc ttttcggcaa
    67861 cgctggaagg cgatgcgatt caggactttg ccgagcgtct gctggaagat ccggtggagg
    67921 tttctgccaa tccgtccacc cgtgagcgca aaaaaattca tcagtggtat taccgcgccg
    67981 atgacctgga acataaaacc gcattgctgg tgcatctgtt aaaacagccg gaagcgaccc
    68041 gctcaattgt gtttgtgcgt aagcgtgagc gtgtgcatga gctggcaaac tggctgcgcg
    68101 aagcgggcat caacaactgc tatctcgaag gtgagatggt acagggcaag cgtaacgaag
    68161 cgatcaaacg tttgaccgaa ggtcgcgtaa acgtactggt tgcaaccgat gttgccgcgc
    68221 gcggtatcga cattcctgac gtcagccacg tctttaactt cgatatgccg cgcagtggcg
    68281 atacttattt gcaccgtatc ggacgtaccg cgcgcgccgg tcgtaaaggc accgcaattt
    68341 cgctggtaga agcccatgac catctgctgc tgggcaaagt tggtcgctat attgaagagc
    68401 caattaaagc tcgcgtaatt gatgagttac gcccgaaaac gcgtgcacca agcgagaagc
    68461 agaccggcaa gccatcgaag aaagtactgg caaaacgcgc cgagaagaaa aaagctaaag
    68521 agaaagagaa gccgcgggtg aaaaaacgcc atcgcgatac caaaaatatt ggtaagcgcc
    68581 gtaaaccaag cggaacgggc gtgccaccgc aaacgacaga agagtaatct caatgccggg
    68641 tttaagcccg gcattatttt tatcccggca aaaaacaatc ttcaacacac tgaataaaag
    68701 tgtgcatcgc cgggctaacc gcttttccgg catggtgagc gcacattgcc gtaatggtct
    68761 gcgagtgttc gccaaaaggc aattcaatta attctccgct ctcaagctct ttttccaccg
    68821 caaagcgggg caagtaactg acaccgatat tcgccgcgac acaacgtttg atactttcaa
    68881 tgcttaacag ctcaatggtg ttttccaccg tgatccgccg ctggcgcagc gtgctctcaa
    68941 atatctgccg gaatacacat tgcggttcgt tgataataaa gctacaggcg ttatgtcttc
    69001 ccggctcagt aaaatcgaca tctgcaattt gcggtgaagc taccagcacc agcgattgct
    69061 cacccaattc tcgtcggttc agggcatcat cattccctac gcgataaaaa accccgacat
    69121 cggcctcatc attcaatagc gcatcacgga tcacgtaaca gttcagagac tgcaacgata
    69181 aacgcacttt tggggcgcgt tgccgaaaac gctgcaacac ctgcggcatt cggtaagaga
    69241 gcaacgtttc gcccgaaaca acgcgtagtt ccccgtccgg atccgactcc tttttggcag
    69301 cctcacgaag ggtatccatc actctggtga gttcataaat gtgcggcagc agctttttac
    69361 cttcgcgagt gaggcacatc cgtcggccaa ttttctcaaa taactgtacc gagaactcct
    69421 gctcaagctg ctgaatatga aaagtcaccg tcgattgtgt acagcacaat ttttgcgaag
    69481 ctcgcaaaaa ggaaccctct tccaccacgg ttttaagcgt aataaaacgg cgcagatcca
    69541 taaccccaaa cctatcgaaa atatcgaatt tagaatgtaa aaacattcat ttttttaaat
    69601 attctgtgtc gggtactgtc taccaaaaca gaggagataa caagtgacac cgaccctttt
    69661 aagtgctttt tggacttaca ccctgattac cgccatgacg ccaggaccga acaatattct
    69721 cgcccttagc tcagccacaa cgcacggatt tcatcagagt acccgcgtgc tggcagggat
    69781 gagtctggga tttttaatcg tcatgttgct gtgtgcgggc atttcgtttt cactggctgt
    69841 tatcgacccg gcagctgtgc atctgttgag ttgggcgggg gcggcatata ttgtctggct
    69901 ggcgtggaaa atcgctacca gtccaacaaa ggaagacggg cttcagacaa aaccaatcag
    69961 tttttgggcc agctttgctt tgcagtttgt gaacgtcaaa atcattttgt acggtgttac
    70021 ggcactgtcg acatttgttc tgccgcaaac acaggcgtta agctggatag ttggcgtcag
    70081 cgttttgctg gcaatgattg ggacgtttgg taatgtgtgc tgggcgctgg cggggcatct
    70141 gtttcagcga ttgtttcgcc agtatggtcg ccagttaaat atcgtgctgg cgctgctgct
    70201 gatctattgc gcggtacgca ttttctatta atgaaaaaaa gcggaagagg tcgccctctt
    70261 ccgcttagta acatgctact taagccttac aggctttcag taaaggtacg agcgataacg
    70321 tcgcgctgct gttccggagt cagagagtta aagcgaactg cataaccgga tacacggatg
    70381 gtcagctgcg gatatttttc cggatgctta actgcatctt ccagagtttc acgacgcaga
    70441 acgttaacgt tcaggtgctg accaccttca acgcgaactt ctggtttcac ttctactgga
    70501 acttcacggt actcaatgtc acccagtttg cttactgcaa ccacttcgtc ttctgcataa
    70561 cctgctttcg caacgatgca acgcgcttcg cctttttcgc tgtccagcag ccagaaagag
    70621 ttcagcagat cgtcgttagc ggctttagta atctggatac ctgtaatcat gtgatgcctc
    70681 cccggcaaaa ttatttgatt tgttcagcct gtcgcggcca attggtaaaa ccattgttgc
    70741 ttgagtgtat atatactcct caaacaccct tgaatctttg atttaaatca ataaaaacca
    70801 cacatcaagt gtggtcgcaa atagatttta ttgttttaca tcaacttatg cgggtgtgaa
    70861 attttaccaa tttacatttt tttgcactcg tttaagtcta aaaaatgagc atgattttgt
    70921 tctgtagaaa gaagcagtta agctaggcgg attgaagatt cgcaggagag cgagatggct
    70981 aacgaattaa cctggcatga cgtgctggct gaagagaagc agcaacccta ttttcttaat
    71041 acccttcaga ccgtcgccag cgagcggcag tccggcgtca ctatctatcc accacaaaaa
    71101 gatgtcttta acgcgttccg ctttaccgag ttgggtgacg ttaaagtggt gattctcggc
    71161 caggatcctt atcacggacc gggacaggcg catgggctgg cattttccgt tcgccccgga
    71221 atcgccactc ctccgtcact gctgaatatg tataaagagc tggaaaatac tattccgggc
    71281 ttcacccgcc ctaatcatgg ttatcttgaa agctgggcgc gtcagggcgt tctgctactc
    71341 aatactgtgt tgacggtacg cgcaggtcag gcgcattccc acgccagcct cggctgggaa
    71401 accttcaccg ataaagtgat cagcctgatt aaccagcatc gcgaaggcgt ggtgtttttg
    71461 ttgtggggat cgcatgcgca aaagaaaggg gcgattatag ataagcaacg ccatcatgta
    71521 ctgaaagcac cgcatccgtc gccgctttcg gctcatcgtg gattctttgg ctgcaaccat
    71581 tttgtgctgg caaatcagtg gctggaacaa catggcgaga cgccgattga ctggatgcca
    71641 gtattaccgg cagagagtga gtaaatttgc gggaaaatgc cggatggcag agttgccacc
    71701 cggctgattt atcaggcttt attctgacgc caccattcgc caagaagaac gccggttgcg
    71761 acagagatat tcagcccggc aacgttgcca gtaccatcaa tcttcacgcg cagatcgttc
    71821 ggatcgcgtg cggcatccgg taacccttca tattcctgac ccagcaccag taccattttc
    71881 gctggcagac tggttttgaa cagcggttta ccctgctcgc tggaagttgt cactacggtg
    71941 taacccgcct gacggaaatc atccagcacg ttaacaatgt tgtcgccagt aatcggctga
    72001 acgtgctctg cgccgccttc tgcggtacgg atagccgccc ccgactccag cagtgccgca
    72061 tcctgcacca caacaccttt cacgccaaag tgcgcgcagc tgcgcatcat gccacccagg
    72121 ttatgcgggt tagattcgtt ttccagtgcc agaacacaat cctgcgcgcc tgcctggctt
    72181 acccactgct gcacggttgt gccattacgc tttttgatca ggaagcaaac gccaccgtga
    72241 tgttccgtgc ctgacgcttt tgtcaattcc gcttcatcta ccacatggta cgctttgcgg
    72301 tttgctgcca tccagcgcaa ggcttcttta aaacgcggcg ttacactctg gataaaccag
    72361 gcgcgaacaa tcgcttccgg acggctctgg aacagtgcct gacaggcgtt ttcgccgtag
    72421 acgcgggttt cttccgcacg ctgacgacgc aacacttccg gatcaataaa acttttacca
    72481 ctgatgccac cgtgatcggc cttttccggc gtctcatcac ccggcgcgcg ggaaaccgtg
    72541 cgccacggcg aatcttccca cttgcggtca cggggctgac tctgtttgtc atcgcgggcg
    72601 gggcgacggc caccgtcagc acgagatttt cctggacgcc cgcccccttt cccggtacgc
    72661 gggttgtggg tacgtttatc agaatcatca tcactgcgga catacatcac tttgaccttg
    72721 ccgcttttac ctttcatttc atcgttcatg cttttctcca ccagcgctgc gcgaagcgcg
    72781 cagattaccc gaagtccgcg cggttcgcca tgatttcgta ccaaagcctg cgactatcat
    72841 acctattgaa taaaacagat tgttgtctgg aacaatgtcc ccgataatat gtaacatatt
    72901 agaaacatac cggcgttgtt gccgataagt ctccttactc atcccgaggt tagttatgaa
    72961 taccgtttgt acccattgtc aggccatcaa tcgcattccc gacgatcgga tcgaagatgc
    73021 ggcaaaatgc ggacgctgcg gtcacgactt gtttgacgga gaggtgatta atgcgaccgg
    73081 tgaaacgctc gacaaattgc tgaaagatga tctaccagtg gtgatcgact tctgggcacc
    73141 gtggtgcggc ccctgccgta atttcgcacc aatttttgaa gatgtcgcgc aagagcgtag
    73201 cggtaaagtg cgctttgtga aagtgaatac cgaagctgaa cgtgaattga gcagtcgctt
    73261 tggaattcgt agtataccga cgatcatgat tttcaaaaac ggtcaggttg tcgacatgct
    73321 taatggcgca gtaccgaaag cgccgttcga tagctggctg aacgaatctc tttaatctta
    73381 ccggggcgca tcttgtgccc cgttttctcc tctgcgacaa tggcgttttt tcgacgctct
    73441 cttatgaccg aaaacgctgt tctccagtta cgcgccgagc gtattgcgcg cgcaacacgt
    73501 ccttttcttg cccgcggtaa tcgcgttcgt cgctgccaac gctgtctttt gccagagaaa
    73561 ttatgtctct gttcgacaat taccccagca caagcaaaaa gtcgcttctg tttgctgatg
    73621 ttcgacaccg agccaatgaa gcccagtaat accgggcgtc tgattgctga tattttgcct
    73681 gataccgttg cgtttcaatg gtcgcgtacc gaaccctcgc aggatttgct ggatctggta
    73741 caaaacccgg attatcagcc aatggtggtc tttcccgcct cgtatgctga tgagcaacgg
    73801 gaagtgatct tcacaccacc tgccggtaag ccaccgctgt ttatcatgct cgatggtacc
    73861 tggccggaag ctcgcaagat gtttcgtaaa agtccgtatc tggataatct tcccgtcatt
    73921 tccgtcgatc tttcccggct ttctgcctat cgcctgcgtg aagcccaggc tgaaggccaa
    73981 tattgtactg ccgaggtagc catcgcactg ttagatatgg ccggcgatac cggggcggca
    74041 gcagggttag gcgagcactt cacccgcttt aaaacgcgct atctggcagg aaaaacgcaa
    74101 catctgggta gcatcacagc agaacagtta gaaagcgttt aaaatcattc ggtcacttct
    74161 gcgggagacc ggtatgagtc agcgaggact ggaagcacta ctgcgaccaa aatcgatagc
    74221 ggtaattggc gcgtcgatga aacccaatcg cgcaggttac ctgatgatgc gtaacctgct
    74281 ggcgggaggc tttaacggac cggtactccc ggtgacgcca gcctggaaag cggtgttggg
    74341 tgtgttggcc tggccggata ttgccagctt gccctttaca cccgaccttg cggttttatg
    74401 taccaatgcc agccgtaatc ttgctcttct ggaagagctc ggcgagaaag gctgtaaaac
    74461 ctgcattatt ctttccgccc cggcatcgca acacgaagat ctccgcgcct gcgccctgcg
    74521 ccataacatg cgcctgcttg gaccaaacag tctgggttta ctggctccct ggcaaggtct
    74581 gaatgccagc ttttcgcctg tgccgattaa acgcggcaag ctggcgttta tttcacagtc
    74641 ggctgccgtc tccaacacca tcctcgactg ggcgcaacag cgtgagatgg gcttttccta
    74701 ctttattgcg ctcggcgaca gcctagatat cgacgttgat gaattgcttg actatctggc
    74761 acgcgacagt aaaaccagcg ccatcctgct ctatctcgaa cagttaagcg acgcgcgacg
    74821 ctttgtttcg gcggcccgta gtgcctcgcg taataaaccg attctggtga ttaaaagcgg
    74881 acgtagcccg gcagcacagc gactgctcaa cacgacggca ggaatggatc cggcgtggga
    74941 tgcggcgatt cagcgtgccg gtttgttgcg ggtgcaggat acccacgagc tgttttcggc
    75001 ggtggaaacc ctcagccata tgcgcccgct gcgtggcgac cggttgatga ttatcagcaa
    75061 cggtgctgcg cctgccgcgc tggcgctgga tgccttatgg tcacgcaatg gcaagctggc
    75121 aacgctaagc gaagaaacct gccagaaact gcgcgatgca ctgccaggac atgtggcaat
    75181 ctctaacccg ctcgatctgc gcgatgacgc cagcagcgag cattatgtca aaacgctgga
    75241 cattctgctc cacagccagg attttgatgc gctgatggtt attcattcgc ccagcgccgc
    75301 tgctcccgca acagaaatcg cgcaagtatt aattgaagcg gtaaagcatc atccccgcag
    75361 caagtatgtt tctctgctga cgaactggtg cggcgagcac tcctcgcaag aggcacgacg
    75421 tttattcagc gaagccgggc tgccgaccta ccgtaccccg gaaggaacca tcactgcttt
    75481 tatgcatatg gtggagtacc ggcgtaatca gaagcaacta cgcgaaacgc cggcgttgcc
    75541 cagcaatctg acttccaata ccgcaaaagc gcatcttctg ttgcaacagg cgattgccga
    75601 aggggctacg tcgctcgata cccatgaagt tcagcccatc ctgcaatcgt atgggatgaa
    75661 cacgctccct acctggattg ccagcgatag caccgaagcg gtgcatattg ccgaacagat
    75721 tggttatccg gtggcgctga aattgcgttc gccggatatt ccacataaat cggaagttca
    75781 gggcgtcatg ctgtacctgc gtacagccaa tgaagtccag caagcggcaa acgctatttt
    75841 cgatcgcgta aaaatgacct ggccgcaggc gcgggtccac ggcctgttgg tgcaaagtat
    75901 ggctaaccgt gctggcgctc aggagttgcg ggttgtggtt gagcacgatc cggtttttgg
    75961 gccgttgatc atgctgggtg aaggcggtgt ggagtggcgt cctgaagatc aagccgtcgt
    76021 cgcactgccg ccgctgaata tgaacctggc ccgctatctg gttattcagg ggatcaaaag
    76081 taaaaagatt cgtgcgcgca gtgcgctacg cccattggat gttgcaggct tgagccagct
    76141 tctggtgcag gtttccaact tgattgtcga ttgcccggaa attcagcgtc tggatattca
    76201 tcctttgctg gcttctggca gtgaatttac cgcgctggat gtcactctgg atatcgcgcc
    76261 gtttgaaggc gataacgaga gtcggctggc agtgcgccct tatccgcatc agctggaaga
    76321 atgggtagaa ttgaaaaacg gtgaacgctg cttgttccgc ccgattttgc cagaagatga
    76381 gccacaactt cagcaattca tttcgcgggt caccaaagaa gatctttatt accgctactt
    76441 tagcgagatc aacgaattta cccatgaaga tttagccaac atgacgcaga tcgactacga
    76501 tcgggaaatg gcgtttgtag cggtacgacg aattgatcaa acggaagaga tcctcggcgt
    76561 cacgcgtgcg atctccgacc ctgataacat cgatgccgaa tttgccgtgc tggttcgctc
    76621 ggatctcaaa gggttaggct tgggtcgacg cttaatggaa aagttgatta cctatacgcg
    76681 agatcacgga ctacaacgtc tgaatggtat tacgatgcca aacaatcgtg gcatggtggc
    76741 gctggcccgc aagctcgggt ttaacgttga tatccagctc gaagagggga tcgttgggct
    76801 tacgctaaat cttgcccaac gcgaggaatc atgagtaagg tactggaaat gttgaccact
    76861 taatcgggac tggtgttatt attgcacgct tatgtcgtct gcattgcaca gaggaccctt
    76921 caatgaacag agaagaaatg cactgtgatg ttgtcaaaat ttaagcgtaa taaacatcaa
    76981 caacaccttg cccaactacc caagatttct caatcagttg atgatgtcga tttcttttac
    77041 gctcccgccg acttccggga gacgctgctg gaaaaaatag ccagcgcgaa gcagcgcatt
    77101 tgcattgtcg ccctgtatct cgaacaggat gacggtggca aaggcattct gaacgcgttg
    77161 tatgaagcta aaaggcagcg tccggaactg gatgtgcggg tgctggtcga ctggcatcgt
    77221 gcacaacgtg gacgcattgg cgctgcggca tctaacacta acgctgactg gtactgtcgc
    77281 atggcgcagg aaaatccggg cgtagatgtt ccggtttatg gcgttccaat caatacccgt
    77341 gaagcccttg gtgttctgca ctttaaaggc tttatcatcg acgatagcgt actttatagc
    77401 ggtgccagcc tgaacgatgt ttacctgcat cagcacgata aatatcgcta cgaccgttat
    77461 catcttatcc gtaaccgtaa gatgtcagac attatgtttg aatgggttac acagaatatt
    77521 atgaatggcc gcggcgttaa tcgtctggat gatgttaatc ggccaaaaag cccggaaatc
    77581 aagaacgata ttcgtctgtt ccgccaggag ctgcgtgatg ccgcttatca tttccagggc
    77641 gatgccgaca acgatcagct ttccgtaacg ccgctagtgg ggctggggaa atcgagtctg
    77701 ttgaacaaga ccattttcca tcttatgcct tgtgccgagc agaaactaac catctgtacg
    77761 ccatacttca acctgccagc aatccttgtg cgcaatatta tccagttgct gcgcgaaggg
    77821 aaaaaggtcg aaattattgt tggtgataaa accgcgaacg acttctacat tccggaagat
    77881 gaacctttca agataattgg cgcattgcct tatctctatg agatcaatct gcgtcgtttc
    77941 ctgagccgtt tgcagtatta cgtcaatact gaccagctag tggttcggtt atggaaagat
    78001 gacgacaaca cctatcacct aaaagggatg tgggttgatg ataagtggat gttgatcacc
    78061 ggtaataacc tgaacccgcg cgcctggcgt ctggatctgg aaaacgccat tttgatccac
    78121 gatccacaac tggagcttgc gccgcagcga gagaaagaac tggagctgat ccgcgagcat
    78181 accaccatcg ttaagcacta tcgcgatctg caaagtattg ccgattatcc ggtgaaggtt
    78241 cgtaaactca tccgccgttt gcgccgtatc cgcatcgacc gattaattag ccgcatcctg
    78301 taatcacaac cccgtcctgt acggggtttg ttttttggag gccacgtttt gcgtattctt
    78361 tttgtctgtt cgctattgtt actttctgga tgcagccata tggctaacga tagctggagc
    78421 gggcaggata aagctcaaca ctttatcgcc tcggcgatgc tttccgccgc cggaaatgaa
    78481 tattcacagc atcaggggat gagccgggat cgcagtgcca tgtttggttt gatgttctct
    78541 gtcagtttgg gggcgtcaaa agagttttgg gatagccgcc ccgaagggag cggctggagc
    78601 tggaaggatt tggcctggga tgtcgccggt gcaagcaccg gctataccgt ctggcaactg
    78661 acccgtcact aaagacgcat ccccttccct ttgcgatgta gcatcaaaga aaccagaaac
    78721 gccaccacgg ccatcaaggt cacataccag aagaaggctg tttccattcc tattgatttc
    78781 agcgacaacg ctacgtactc cgccgaacca ccaaatatag cattagcgac cgcatatgac
    78841 agaccaacgc ctaatgcgcg aacctgtgcc gggaacatct cagccttcag tattccactg
    78901 attgatgtat aaaaactcac tatcagcagg gcacacatca ccagaccaaa agcggcataa
    78961 ggcgaggaaa cgttttgcaa tgctgagaga ataggaacgg taaaaatggc tgccagcgaa
    79021 ccgaaacata acattgaggt acggcgacca atcttatccg acagcgcgcc aatgagtggt
    79081 tgaataagca tgaatacaaa caatgcggca gtcataatgc cactcgccac gttggcatgc
    79141 attcccgcag tatttaccag atacttctgc atataagtgg tgaaggtata gaaacaaagg
    79201 gagcccgcag cggtaaaacc gagaaccatg atgaatgcac ggcgattgcg ccataatcct
    79261 ttcagagatc cagcttcttt taaagcgcgc gtttcttgtt gcgaagtttc atctaactga
    79321 cgacgtaacc acaacgccac aacagctaac acagctccta acgcgaaagg aatacgccat
    79381 ccccactctc tgagtgcaga gtcttccatg gtgtgttgta aaaccacgac aaccagtagg
    79441 gctagcagtt gtccgccgat caacgtcaca tactgaaatg atgcgtaaaa acctttgcgc
    79501 ccttcaacgg caacttcact catataggtg gcgctggtgc catattctcc gccaacggat
    79561 aatccctgaa ataaacgcgc gagaagcaat aatgccggag cccacgtacc gattgtttca
    79621 taaccaggca aacaggcgat taccagtgag ccgaaacaca tcatacacac cgatagcagc
    79681 atcgattttt tgcgaccatg tttatcggct atgcggccaa atagccaacc gcctattggg
    79741 cgcatcagga atcccgcagc aaaaacacct gctgtttgta gtagttgagt cgtcgtgttc
    79801 ccggaaggga agaagatgtg ggcaaagtag agtgaacaga acgagtagac atagaaatcg
    79861 aaccactcga ccagattacc tgaagaggcc cccacaatcg cccaaatgcg gcgacgagta
    79921 tcactacttg tcagtttgct gtctgccgtt acagtacttt cagccatgcc attatgtctc
    79981 ctgccgtaat ccgatgcttt tgtcggtcgc ttttgtttat ttcttttggc aaagaaaatg
    80041 ttatacattt gttacgtatc attatgcaac cttaaccatg aatttagtta gcaggaaaat
    80101 ggttattgag gagcttaagg ataaatttct ggtaaggagg acacgtatgg aagtaggcaa
    80161 gttggggaaa ccgtatccgt tgctgaatct ggcatatgtg ggagtataag acgcgcagcg
    80221 tcgcatcagg catttttttc accaacgcaa aaaggccatc ctgacggatg gccttt
//
LOCUS       DAZKIP010000008        80113 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZKIP010000008 DAZKIP010000000
VERSION     DAZKIP010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 80113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.008"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            78..347
                     /locus_tag="ACHOUK_000629"
     CDS             78..347
                     /locus_tag="ACHOUK_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFP9896140.1"
                     /translation="MSEQYLITLDEWKPKRFSLPITNTTLVKYGKLGYIVPRPQKIRG
                     RWLIDRRAVFVGPGETGIAPEIHTGDDDALKEILTHVTEATKKQH"
     gene            316..1434
                     /locus_tag="ACHOUK_000630"
     CDS             316..1434
                     /locus_tag="ACHOUK_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFP9896141.1"
                     /translation="MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTD
                     KGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKN
                     GDIAATTHKEKTRMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLW
                     VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTMEDWQKIYNATPEKHFIRNAML
                     LAIVTGQRRDDICHMRFSDVWNEHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGC
                     RDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGVIPPAGKTPPTFHEQRSLSE
                     RLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWVKLVI"
     gene            1601..2395
                     /gene="potC"
                     /locus_tag="ACHOUK_000631"
     CDS             1601..2395
                     /gene="potC"
                     /locus_tag="ACHOUK_000631"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFP9896142.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            2392..3438
                     /gene="potD"
                     /locus_tag="ACHOUK_000632"
     CDS             2392..3438
                     /gene="potD"
                     /locus_tag="ACHOUK_000632"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFP9896143.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLSPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(3594..4415)
                     /gene="cobB"
                     /locus_tag="ACHOUK_000633"
     CDS             complement(3594..4415)
                     /gene="cobB"
                     /locus_tag="ACHOUK_000633"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFP9896144.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(4431..5342)
                     /gene="nagK"
                     /locus_tag="ACHOUK_000634"
     CDS             complement(4431..5342)
                     /gene="nagK"
                     /locus_tag="ACHOUK_000634"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFP9896145.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLPAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(5371..6615)
                     /gene="lolE"
                     /locus_tag="ACHOUK_000635"
     CDS             complement(5371..6615)
                     /gene="lolE"
                     /locus_tag="ACHOUK_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFP9896146.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(6615..7316)
                     /gene="lolD"
                     /locus_tag="ACHOUK_000636"
     CDS             complement(6615..7316)
                     /gene="lolD"
                     /locus_tag="ACHOUK_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFP9896147.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPWLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(7309..8508)
                     /gene="lolC"
                     /locus_tag="ACHOUK_000637"
     CDS             complement(7309..8508)
                     /gene="lolC"
                     /locus_tag="ACHOUK_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFP9896148.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            8770..9843
                     /gene="ycfT"
                     /locus_tag="ACHOUK_000638"
     CDS             8770..9843
                     /gene="ycfT"
                     /locus_tag="ACHOUK_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFP9896149.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLAALAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPVAVSYSR"
     gene            9971..13417
                     /gene="mfd"
                     /locus_tag="ACHOUK_000639"
     CDS             9971..13417
                     /gene="mfd"
                     /locus_tag="ACHOUK_000639"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFP9896150.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMEKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLENSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLESFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDTVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     LSRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            13561..14523
                     /gene="ldtC"
                     /locus_tag="ACHOUK_000640"
     CDS             13561..14523
                     /gene="ldtC"
                     /locus_tag="ACHOUK_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFP9896151.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTLPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(14606..14863)
                     /gene="bhsA"
                     /locus_tag="ACHOUK_000641"
                     /gene_synonym="ycfR"
     CDS             complement(14606..14863)
                     /gene="bhsA"
                     /locus_tag="ACHOUK_000641"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFP9896152.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            15104..15736
                     /gene="comR"
                     /locus_tag="ACHOUK_000642"
     CDS             15104..15736
                     /gene="comR"
                     /locus_tag="ACHOUK_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFP9896153.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(15798..16337)
                     /gene="ycfJ"
                     /locus_tag="ACHOUK_000643"
     CDS             complement(15798..16337)
                     /gene="ycfJ"
                     /locus_tag="ACHOUK_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFP9896154.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDEHRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(16564..17868)
                     /gene="ndh"
                     /locus_tag="ACHOUK_000644"
     CDS             complement(16564..17868)
                     /gene="ndh"
                     /locus_tag="ACHOUK_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFP9896155.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDRDAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVELTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKLLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(18268..18810)
                     /gene="ycfP"
                     /locus_tag="ACHOUK_000645"
     CDS             complement(18268..18810)
                     /gene="ycfP"
                     /locus_tag="ACHOUK_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFP9896156.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(18833..19858)
                     /gene="nagZ"
                     /locus_tag="ACHOUK_000646"
     CDS             complement(18833..19858)
                     /gene="nagZ"
                     /locus_tag="ACHOUK_000646"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFP9896157.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPEKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSTLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQVSLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISARLNQLHERW
                     QEEKAGH"
     gene            complement(19869..20693)
                     /gene="thiK"
                     /locus_tag="ACHOUK_000647"
     CDS             complement(19869..20693)
                     /gene="thiK"
                     /locus_tag="ACHOUK_000647"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFP9896158.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(20674..21315)
                     /gene="lpoB"
                     /locus_tag="ACHOUK_000648"
     CDS             complement(20674..21315)
                     /gene="lpoB"
                     /locus_tag="ACHOUK_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFP9896159.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(21329..21706)
                     /gene="ycfL"
                     /locus_tag="ACHOUK_000649"
     CDS             complement(21329..21706)
                     /gene="ycfL"
                     /locus_tag="ACHOUK_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFP9896160.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(21709..22068)
                     /gene="hinT"
                     /locus_tag="ACHOUK_000650"
     CDS             complement(21709..22068)
                     /gene="hinT"
                     /locus_tag="ACHOUK_000650"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFP9896161.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            22408..24597
                     /gene="fhuE"
                     /locus_tag="ACHOUK_000651"
     CDS             22408..24597
                     /gene="fhuE"
                     /locus_tag="ACHOUK_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFP9896162.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(24657..26090)
                     /gene="ptsG"
                     /locus_tag="ACHOUK_000652"
     CDS             complement(24657..26090)
                     /gene="ptsG"
                     /locus_tag="ACHOUK_000652"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFP9896163.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(26385..27182)
                     /gene="ycfH"
                     /locus_tag="ACHOUK_000653"
     CDS             complement(26385..27182)
                     /gene="ycfH"
                     /locus_tag="ACHOUK_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP9896164.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(27193..28197)
                     /gene="holB"
                     /locus_tag="ACHOUK_000654"
     CDS             complement(27193..28197)
                     /gene="holB"
                     /locus_tag="ACHOUK_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFP9896165.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(28194..28835)
                     /gene="tmk"
                     /locus_tag="ACHOUK_000655"
     CDS             complement(28194..28835)
                     /gene="tmk"
                     /locus_tag="ACHOUK_000655"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFP9896166.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEIITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTRWVKELDA"
     gene            complement(28825..29847)
                     /gene="yceG"
                     /locus_tag="ACHOUK_000656"
     CDS             complement(28825..29847)
                     /gene="yceG"
                     /locus_tag="ACHOUK_000656"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFP9896167.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRYLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYITVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAIASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(29850..30659)
                     /gene="pabC"
                     /locus_tag="ACHOUK_000657"
     CDS             complement(29850..30659)
                     /gene="pabC"
                     /locus_tag="ACHOUK_000657"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFP9896168.1"
                     /translation="MFLINGYKQESLSVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGITLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGGVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(30779..32020)
                     /gene="fabF"
                     /locus_tag="ACHOUK_000658"
     CDS             complement(30779..32020)
                     /gene="fabF"
                     /locus_tag="ACHOUK_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFP9896169.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(32108..32344)
                     /gene="acpP"
                     /locus_tag="ACHOUK_000659"
     CDS             complement(32108..32344)
                     /gene="acpP"
                     /locus_tag="ACHOUK_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFP9896170.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(32555..33289)
                     /gene="fabG"
                     /locus_tag="ACHOUK_000660"
     CDS             complement(32555..33289)
                     /gene="fabG"
                     /locus_tag="ACHOUK_000660"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFP9896171.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILVQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(33302..34231)
                     /gene="fabD"
                     /locus_tag="ACHOUK_000661"
     CDS             complement(33302..34231)
                     /gene="fabD"
                     /locus_tag="ACHOUK_000661"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFP9896172.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQHGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIGKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(34247..35200)
                     /gene="fabH"
                     /locus_tag="ACHOUK_000662"
     CDS             complement(34247..35200)
                     /gene="fabH"
                     /locus_tag="ACHOUK_000662"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFP9896173.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(35268..36338)
                     /gene="plsX"
                     /locus_tag="ACHOUK_000663"
     CDS             complement(35268..36338)
                     /gene="plsX"
                     /locus_tag="ACHOUK_000663"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFP9896174.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(36419..36592)
                     /gene="rpmF"
                     /locus_tag="ACHOUK_000664"
     CDS             complement(36419..36592)
                     /gene="rpmF"
                     /locus_tag="ACHOUK_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFP9896175.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(36644..37165)
                     /gene="yceD"
                     /locus_tag="ACHOUK_000665"
     CDS             complement(36644..37165)
                     /gene="yceD"
                     /locus_tag="ACHOUK_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFP9896176.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            37364..37948
                     /gene="yceF"
                     /locus_tag="ACHOUK_000666"
     CDS             37364..37948
                     /gene="yceF"
                     /locus_tag="ACHOUK_000666"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFP9896177.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPHSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(38049..39008)
                     /gene="rluC"
                     /locus_tag="ACHOUK_000667"
     CDS             complement(38049..39008)
                     /gene="rluC"
                     /locus_tag="ACHOUK_000667"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFP9896178.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDDGLKRCLQKLRNAR"
     gene            39581..42766
                     /gene="rne"
                     /locus_tag="ACHOUK_000668"
     CDS             39581..42766
                     /gene="rne"
                     /locus_tag="ACHOUK_000668"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFP9896179.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGGEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETREGRQQAEVTEKARTADEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEETV
                     VAPVAEETVAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVTAVKPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(42961..43914)
                     /gene="flgL"
                     /locus_tag="ACHOUK_000669"
     CDS             complement(42961..43914)
                     /gene="flgL"
                     /locus_tag="ACHOUK_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFP9896180.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDNDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEVNGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(43926..45569)
                     /gene="flgK"
                     /locus_tag="ACHOUK_000670"
     CDS             complement(43926..45569)
                     /gene="flgK"
                     /locus_tag="ACHOUK_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFP9896181.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDEGEDFFAIGKPA
                     VLQNTKNNGNVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(45635..46576)
                     /gene="flgJ"
                     /locus_tag="ACHOUK_000671"
     CDS             complement(45635..46576)
                     /gene="flgJ"
                     /locus_tag="ACHOUK_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFP9896182.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTTGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(46576..47673)
                     /gene="flgI"
                     /locus_tag="ACHOUK_000672"
     CDS             complement(46576..47673)
                     /gene="flgI"
                     /locus_tag="ACHOUK_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309485.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFP9896183.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNVKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAIA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(47685..48383)
                     /gene="flgH"
                     /locus_tag="ACHOUK_000673"
     CDS             complement(47685..48383)
                     /gene="flgH"
                     /locus_tag="ACHOUK_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFP9896184.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(48436..49218)
                     /gene="flgG"
                     /locus_tag="ACHOUK_000674"
     CDS             complement(48436..49218)
                     /gene="flgG"
                     /locus_tag="ACHOUK_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFP9896185.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(49356..50111)
                     /gene="flgF"
                     /locus_tag="ACHOUK_000675"
     CDS             complement(49356..50111)
                     /gene="flgF"
                     /locus_tag="ACHOUK_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFP9896186.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADITPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(50131..51336)
                     /gene="flgE"
                     /locus_tag="ACHOUK_000676"
     CDS             complement(50131..51336)
                     /gene="flgE"
                     /locus_tag="ACHOUK_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFP9896187.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPSVNAFDASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGAEPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(51361..52056)
                     /gene="flgD"
                     /locus_tag="ACHOUK_000677"
     CDS             complement(51361..52056)
                     /gene="flgD"
                     /locus_tag="ACHOUK_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFP9896188.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(52068..52472)
                     /gene="flgC"
                     /locus_tag="ACHOUK_000678"
     CDS             complement(52068..52472)
                     /gene="flgC"
                     /locus_tag="ACHOUK_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFP9896189.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(52476..52892)
                     /gene="flgB"
                     /locus_tag="ACHOUK_000679"
     CDS             complement(52476..52892)
                     /gene="flgB"
                     /locus_tag="ACHOUK_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFP9896190.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRNATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            53047..53706
                     /gene="flgA"
                     /locus_tag="ACHOUK_000680"
     CDS             53047..53706
                     /gene="flgA"
                     /locus_tag="ACHOUK_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFP9896191.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLQTFFSSQLAGISDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLGAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            53782..54075
                     /gene="flgM"
                     /locus_tag="ACHOUK_000681"
     CDS             53782..54075
                     /gene="flgM"
                     /locus_tag="ACHOUK_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFP9896192.1"
                     /translation="MSIDRTSPLKPVSTIQPRETTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            54080..54496
                     /gene="flgN"
                     /locus_tag="ACHOUK_000682"
     CDS             54080..54496
                     /gene="flgN"
                     /locus_tag="ACHOUK_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFP9896193.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEIAVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGSKKISI"
     gene            complement(54536..56071)
                     /gene="murJ"
                     /locus_tag="ACHOUK_000683"
     CDS             complement(54536..56071)
                     /gene="murJ"
                     /locus_tag="ACHOUK_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFP9896194.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGVLQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(56181..57104)
                     /gene="yceM"
                     /locus_tag="ACHOUK_000684"
     CDS             complement(56181..57104)
                     /gene="yceM"
                     /locus_tag="ACHOUK_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9896195.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYCELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITEMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(57106..57753)
                     /gene="yceH"
                     /locus_tag="ACHOUK_000685"
     CDS             complement(57106..57753)
                     /gene="yceH"
                     /locus_tag="ACHOUK_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFP9896196.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVELTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(57764..58348)
                     /gene="rimJ"
                     /locus_tag="ACHOUK_000686"
     CDS             complement(57764..58348)
                     /gene="rimJ"
                     /locus_tag="ACHOUK_000686"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFP9896197.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            58584..59792
                     /gene="mdtH"
                     /locus_tag="ACHOUK_000687"
     CDS             58584..59792
                     /gene="mdtH"
                     /locus_tag="ACHOUK_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFP9896198.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGDAIGYIGGGWLFDLGKSVHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            59856..60503
                     /gene="grxB"
                     /locus_tag="ACHOUK_000688"
     CDS             59856..60503
                     /gene="grxB"
                     /locus_tag="ACHOUK_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFP9896199.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            60637..61197
                     /gene="yceB"
                     /locus_tag="ACHOUK_000689"
     CDS             60637..61197
                     /gene="yceB"
                     /locus_tag="ACHOUK_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP9896200.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVALTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            61303..62349
                     /gene="pyrC"
                     /locus_tag="ACHOUK_000690"
     CDS             61303..62349
                     /gene="pyrC"
                     /locus_tag="ACHOUK_000690"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFP9896201.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILHAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSVDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEHQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            62423..62668
                     /gene="dinI"
                     /locus_tag="ACHOUK_000691"
     CDS             62423..62668
                     /gene="dinI"
                     /locus_tag="ACHOUK_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFP9896202.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            62958..63212
                     /gene="bssS"
                     /locus_tag="ACHOUK_000692"
     CDS             62958..63212
                     /gene="bssS"
                     /locus_tag="ACHOUK_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFP9896203.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            63327..64445
                     /gene="solA"
                     /locus_tag="ACHOUK_000693"
     CDS             63327..64445
                     /gene="solA"
                     /locus_tag="ACHOUK_000693"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFP9896204.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDDLSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFSLSRFQ"
     gene            64493..64606
                     /locus_tag="ACHOUK_000694"
     CDS             64493..64606
                     /locus_tag="ACHOUK_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFP9896205.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            64867..65433
                     /gene="yceJ"
                     /locus_tag="ACHOUK_000695"
     CDS             64867..65433
                     /gene="yceJ"
                     /locus_tag="ACHOUK_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFP9896206.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            65437..66012
                     /gene="yceI"
                     /locus_tag="ACHOUK_000696"
     CDS             65437..66012
                     /gene="yceI"
                     /locus_tag="ACHOUK_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFP9896207.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(66054..67106)
                     /gene="trhO"
                     /locus_tag="ACHOUK_000697"
     CDS             complement(66054..67106)
                     /gene="trhO"
                     /locus_tag="ACHOUK_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFP9896208.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVKTFRAQLYAFDSALDGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            67331..68251
                     /gene="lpxL"
                     /locus_tag="ACHOUK_000698"
     CDS             67331..68251
                     /gene="lpxL"
                     /locus_tag="ACHOUK_000698"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFP9896209.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            68423..69649
                     /gene="mdtG"
                     /locus_tag="ACHOUK_000699"
                     /pseudo
     CDS             68423..69649
                     /gene="mdtG"
                     /locus_tag="ACHOUK_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            69732..70106
                     /gene="msyB"
                     /locus_tag="ACHOUK_000700"
     CDS             69732..70106
                     /gene="msyB"
                     /locus_tag="ACHOUK_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFP9896210.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(70107..70334)
                     /gene="yceK"
                     /locus_tag="ACHOUK_000701"
     CDS             complement(70107..70334)
                     /gene="yceK"
                     /locus_tag="ACHOUK_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFP9896211.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(70507..73050)
                     /gene="mdoH"
                     /locus_tag="ACHOUK_000702"
     CDS             complement(70507..73050)
                     /gene="mdoH"
                     /locus_tag="ACHOUK_000702"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFP9896212.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(73043..74578)
                     /gene="mdoG"
                     /locus_tag="ACHOUK_000703"
     CDS             complement(73043..74578)
                     /gene="mdoG"
                     /locus_tag="ACHOUK_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFP9896213.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            74971..76128
                     /gene="mdoC"
                     /locus_tag="ACHOUK_000704"
     CDS             74971..76128
                     /gene="mdoC"
                     /locus_tag="ACHOUK_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFP9896214.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGTFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(76136..77617)
                     /gene="clsC"
                     /locus_tag="ACHOUK_000705"
     CDS             complement(76136..77617)
                     /gene="clsC"
                     /locus_tag="ACHOUK_000705"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309451.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFP9896215.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMSEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIVLKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(77559..78092)
                     /gene="ymdB"
                     /locus_tag="ACHOUK_000706"
     CDS             complement(77559..78092)
                     /gene="ymdB"
                     /locus_tag="ACHOUK_000706"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFP9896216.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(78187..78498)
                     /gene="ymdA"
                     /locus_tag="ACHOUK_000707"
     CDS             complement(78187..78498)
                     /gene="ymdA"
                     /locus_tag="ACHOUK_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFP9896217.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(78619..78951)
                     /gene="csgC"
                     /locus_tag="ACHOUK_000708"
     CDS             complement(78619..78951)
                     /gene="csgC"
                     /locus_tag="ACHOUK_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFP9896218.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(79010..79468)
                     /gene="csgA"
                     /locus_tag="ACHOUK_000709"
     CDS             complement(79010..79468)
                     /gene="csgA"
                     /locus_tag="ACHOUK_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFP9896219.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGGNHGGGGNNSGPNS
                     ELNIYQYGGGNSALALQADARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSA
                     TLDQWNGKDSHMTVKQFGGGNGAAVDQTASNSTVNVTQVGFGNNATAHQY"
     gene            complement(79509..79964)
                     /gene="csgB"
                     /locus_tag="ACHOUK_000710"
     CDS             complement(79509..79964)
                     /gene="csgB"
                     /locus_tag="ACHOUK_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFP9896220.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT        19646 a        21180 c        19812 g        19475 t
ORIGIN      
        1 tcaggctgat ttttaatgtt caaccgtcgc cagttgtcgt tgatattctg caactggcgc
       61 gttccggagt gatagccatg agcgaacagt acctgataac gctcgacgag tggaaaccaa
      121 aacggttcag tctcccaata acaaacacta ccctggtgaa atacggaaaa ctaggataca
      181 tcgttccaag accacaaaaa attcgtgggc gttggctgat agatcgccga gcagtatttg
      241 ttgggcctgg tgaaacggga attgcgccgg aaattcatac tggcgatgat gatgcactga
      301 aggagatttt aactcatgtc accgaggcca cgaaaaaaca gcactgacgt agccggtctt
      361 tacgaaaagt ttgatcgcag aactggcaga gtttactacc agtataaaaa tcctgtgact
      421 ggaaaatttc acggactcgg aacagacaaa ggtaaggcag aaaaaatcgc ttccacagcc
      481 aatcagcgaa tagctgcagc agaagctgaa tatttcatgc gcaaaattga tgaaagtccg
      541 tcagcaacaa aacgtcgggg tatcagatta aaggcatggg ttgatcgata tctgaaaata
      601 caggacacgc gactgaaaaa tggagatatt gcagctacaa ctcacaaaga aaaaactcga
      661 atggctgcat acctggtttc ccgtctggga aaccacccat tgaaagaact ggaagtaaga
      721 gactttgcat taatactgga tgagtggctg gataaagaca tggtcagtac agcgagagta
      781 aatcgtggat tatgggttga tatttataaa gaagcacagc atgcagggga agttcctcct
      841 ggatggaatc ctccggaggc tacccgtaaa ccgatcccta aagtaaccag agccaggctc
      901 accatggaag actggcaaaa aatttacaat gcaacgcctg aaaaacactt tatccgtaac
      961 gcaatgcttc ttgcgattgt tactggtcag cgccgtgatg acatttgcca catgcgtttt
     1021 tcagatgtgt ggaacgaaca cttgcatatc acccagggaa aaaccggaat gcgtctggcg
     1081 ttaccgctta cactacgctg tgatgccatt gggataacgt taaaagaagt tattgatggg
     1141 tgccgagaca gaatattaag tccatatcta atccatagtc ggcaccagaa acaaccgaag
     1201 ccgatgagta aagacaacct gagcgactac tttgccaaag cacgggatct ggctggggta
     1261 attccaccag caggaaaaac tccgccaaca tttcatgaac aacgctctct atcagaacgg
     1321 ctgtaccgtg cacagggtat cgatacaaaa acattactag gacataaagt ccaggcaacc
     1381 accgatcgct ataacgatac tcgaggtcag gaatgggtta agttggttat ttgacaaaca
     1441 ccatatggaa tatccgtcac catctcagga aaaaagtgtt gtaaaatgcg tggcctagtt
     1501 ttggggaaac gttttggaga gattttggag aaaggaaaaa attgaataaa ttcaaaatcc
     1561 tgaaagtgtc ccgccttatg aataaaaagg tggaactcga atgatcggtc gactgcttcg
     1621 cggcggtttt atgaccgcta tctacgcgta cctgtatatc ccaatcatta ttttgattgt
     1681 gaactccttt aacagctcgc gctttggcat caactggcag ggttttacca ccaaatggta
     1741 tagcctgctg atgaacaacg acagcctgtt acaggcagcg cagcattcac taacaatggc
     1801 ggtgttttcg gcgacgtttg ctacgcttat cggttcactg acggcagttg cgctgtaccg
     1861 ttatcgcttt cgtggtaagc cgttcgttag cggaatgctg tttgtggtga tgatgtcacc
     1921 agatatcgtg atggcgattt cgctgctggt gctgtttatg ctgctgggta ttcagcttgg
     1981 cttctggtcg ctgctgttct cgcatatcac cttctgcctg ccatttgtgg tggtgacggt
     2041 gtattcgcgc ctgaaaggtt ttgacgtgcg gatgctggaa gcggcgaaag atctcggtgc
     2101 cagcgaattt accattctgc gaaaaatcat tctgccactg gcaatgccag cggtggcggc
     2161 aggctgggtg ttaagcttta ccctgtcgat ggacgatgtg gtggtttctt cgttcgtcac
     2221 cgggccgagt tatgaaattc tgccgttaaa aatttattcg atggtcaaag tcggcgtatc
     2281 gccggaagtt aacgcgctgg caaccatatt actggtgctg tcgctggtga tggttattgc
     2341 cagccagctt attgctcgtg ataaaacgaa aggtaacaca ggggacgtta aatgaaaaaa
     2401 tggtcacgcc acctgctcgc ggcgggtgct ctggcactgg gcatgagcgc cgctcacgcc
     2461 gatgacaaca acacgctgta tttctacaac tggaccgagt acgtgccgcc aggactgctt
     2521 gaacagttca ccaaagaaac cggtattaag gttatctatt cgacttacga gtcgaacgaa
     2581 accatgtatg ccaagctgaa aacgtacaaa gacggtgcct atgacctggt ggttccttca
     2641 acctattacg tcgataaaat gcgtaaagaa gggatgatcc agaagatcga caagtcgaag
     2701 ttaacgaact tcagcaatct cgatccagac atgctcaata agccgtttga cccgaataac
     2761 gattactcca ttccgtatat ctggggtgcg acggcgattg gcgttaacgg tgatgcggtg
     2821 gatccgaaat ctgtcaccag ctgggccgat ttgtggaagc ctgagtacaa aggcagcctg
     2881 ctgctgaccg acgatgcccg tgaagtgttc cagatggcgc tacgtaagct gggctactcc
     2941 ggtaacacca ccgatccgaa agagattgaa gctgcatata acgagctgaa aaaactgatg
     3001 ccaaacgtcg cagcgtttaa ctccgataac ccggctaacc cgtacatgga aggcgaagtt
     3061 aacctcggca tgatctggaa cggttctgct ttcgttgcac gccaggcggg tacgcctatt
     3121 gacgtggtgt ggccgaaaga aggcggcata ttctggatgg acagcctggc gatcccggca
     3181 aatgccaaaa acaaagaagg cgcgctgaaa ttgatcaact tcctgctgcg cccggatgtg
     3241 gcaaaacagg ttgctgaaac tatcggttat ccaacgccaa accttgcggc gcgtaagctg
     3301 ttaagtccag aagtggcgaa cgataagacg ctctccccgg atgctgaaac cattaaaaat
     3361 ggcgaatggc agaatgacgt tggcgcagcc agcagcattt atgaagagta ttatcagaag
     3421 ctgaaagcag gacgttaatt cagcaagcgg gcggtggtat taccgcccgt tttttagtta
     3481 gcaaggatgt ggctaatgag tcaggacagt aaagttttat cccgctcttt cgtcggtatt
     3541 gtggtccgct tgtaggcctg ataagcgtag cgcatcaggc aatgcttcct gctttataat
     3601 cccttcagca acttttcaac aaattctggc accacctggc ttgccgggcc gtaatatttc
     3661 tcggcaaatt cattaccgac ctgacttggt tcaagattca gctccacggt gtgcgcgcca
     3721 tgcagtttcg cttcgtgaac aaacccagcc gccggataaa catgcccgga agtaccaatg
     3781 gcaatgaaaa tatcggccat cgacaacgcc atataaattt catccatgcc gagtggcatt
     3841 tcgccaaacc atactacgtg tgggcgcaag ggggccggga actggcagca atggcattta
     3901 tcttctgggg taacgtctcc ggtccagtcg agaacctgac cactttgtga acaacgaact
     3961 ttcagcagtt ccccatgcat atgaatcaca ttggtattac ctgcgcgttc atgcaggttg
     4021 tctatattct gcgtcaccag caaaaagcga tcgccgagag catcttgcag tttagccagc
     4081 gcaagatgcg cggcgttagg ctgaatttct ggctgctgca gctgtcgacg acgggcgtta
     4141 taaaacgctt gcaccagttc aggatcgcga tcgaaacctt ccggcgttgc cacatcttca
     4201 acccgatgtt cttcccacag gccatctgcg gcgcgaaagg tacgaatacc tgattccgca
     4261 gaaattcctg cccctgtcag tacgagtact cttggttttt ccattgcttc cggcaccact
     4321 ttatctctga aaaaaatacg ctgacgcaaa cgctcgcgca ggcggcgttt atttttacga
     4381 aaacgactta accgatgacc ccgacgcgac agcatagcaa cctctgttgt ttaatcggtt
     4441 agatgtagga aggccgcacc acgcattcct cccgcatcac cgtggcgcgc gcgttcaatg
     4501 cgcggaacac gagctacagg taagagatga cgaggcagcc tgtccgccag ttgcgttgtg
     4561 attgccggga aattcgataa gccaccacca atgacgacca ggtcagggtc aacaatggtc
     4621 aggatatttc ccagacaaac cgctaataaa tccagataac gctcaacgtg cgcccttgcc
     4681 tgctcatcgc cttgatcata aagcgcaata atttcgggag ccggcaacgg ttgatgataa
     4741 tagtgttgat acagccacgc aaaaccgcga ccagacagat aattttcaat gcagccatgc
     4801 tgaccacagc cgcagcggcg taacgggaaa tccagcccca tcatggttaa cgcatcaacc
     4861 ggcagacgca tatggccaaa ctcgccggta atgtagcttt tcccggtaat cggtttgccg
     4921 ttgaaaatca gcccgccgcc aacgccggtg ccgagaatca accccatcac caacggatat
     4981 tgcgtaaatt cgtcatccca ggcttctgaa agggcaaaac agttggcatc gttatcaagg
     5041 cgtacatcgc gatcaagacg tgcgctcagg tcggcacgca gcggtttacc gctggcagca
     5101 gggacattgg cggcatacag cgtaccatct tctgtttccg gcattcccgg aataccgatg
     5161 ccgacagagc ctttacagcc aaaacgttga tcagcttcgg ctaccagctc gcacactgca
     5221 tctaaaaatg cgtcatagct gtcacgcggt gtcggcaccc gcttttccca ctgcaactgc
     5281 cgaccgctat caaacacgcc aagcgcaatt tttgttccac caatatcaaa cccgtaatac
     5341 attgccgctc cttgttttaa tgtactgcct ttactggccg ctaaggactc gcgcagggtc
     5401 aatattactg gcgcgccgcg ccggatacca acttgccaaa agactcagca acaatgctgt
     5461 gaccagtacg tagaagacgt ccagccagtg caattccgat ggcaggaagt caataaaata
     5521 gatatcgctg gagaggaact gatgaccgat cagcttttca atccgctcaa taatcggggt
     5581 aagttgcagt gaaacaacta cgccgataat cacaccacac aggctaccga atagccctgc
     5641 cagcaatcca taccagacaa agatggcgcg aattaaacca tctttcgccc ccagcgttct
     5701 taatactgcg atatcgccac tcttgtcttt caccgccatc actaaggtgg agacgatgtt
     5761 gaaacaggcc acgccaatca ccagtaccat cgccagatac ataatggcgc ggatcatctg
     5821 gatatcgcga tacatatagc cgtaagtacc aatccagctt ttaatataaa catagctgtt
     5881 ggtcacttca cccgcatcgc gtaccagctt attggcgttg aaaacatccg tcattttaag
     5941 ggcaatacct gacacgctgg aacccatatc aagatattgt tgggcatccg ccagcgggat
     6001 catggcaaaa ctgtgatcga gttgaccact caactgcaaa ataccggcaa cgtgcaaacg
     6061 cacacgtttt ggctgcatca gtttatgctc aggattcgag ttggggatca taatcgacac
     6121 ccaatcgccc tgcttcactt tcagcgcatc cgccacgcct ttgccgatga taatttgctg
     6181 ttcgcccgct ttaaaattgc gccaggcatc cccctgaaca aacgagggta atgcgctcag
     6241 acgctgttcc tgttgcgggt taacgccctt cacctggatt gcgcgcagat tcgctccact
     6301 ttccaccagc ccggtgaaat tgatatacgg cgcagcggcg gcaatacctg gcactttttg
     6361 cacgttatcc agtgcttcct gccagttagt ccacggttga tccaccgcct ctatttcgcc
     6421 atgcggcacc accgccagaa tgcggttatt cagttcgcgt tcaaagccgt tcatcgcgct
     6481 taagccgacg atcaataccg ccacgccaag ggcaatgcca atggtagaaa tgacggagat
     6541 cagcgacacc atgccgccgc gtcgccgtcc gcggctaaaa cgcaggccaa tcaataacga
     6601 taaaggcatc gccattactc cgcccccatc aggctcagtt ccgccgtcag acgaccatca
     6661 cgcatctcca gttggcggct catacgtttc gccagttgca ggtcgtgagt aaccaccagg
     6721 aaggcggtgc cctgcaagcg attcaattcc ccaagcaact ggaagatgct gtctgcgtta
     6781 cgcgcatcga ggttaccggt aggttcatcc gccagtacca gccacgggtt attgaccagc
     6841 gcacgggcaa tcgccacacg ctggcgttcg ccgccagaaa gttcagatgg gcggtgattc
     6901 gcacgatgct ccagccccac cgcttttaac atctcaagtg cacggctgtt gatttcagcg
     6961 ggctttttct tgccaatcag cagcggcata gccacgtttt ccagggcagt aaaatccggc
     7021 agcaggtggt gaaactgata aataaagccc agcttctggt tgcgcagttc tgctttcgcc
     7081 gccgaagaca gtttgctcat cggttgaccg ttaaagatca catcgccgga ggttggtgta
     7141 tccagcccgc ccagcaggtg cagcaaggta cttttaccgg aaccagagct accgacgatc
     7201 gccatcattt caccttcgcc cacgctgaaa ctgacattgt gcagtacatc ggtttgcaca
     7261 ctgccttcct gatagcgttt gcacaggttg tcgcattgca acaggatctt attcataacg
     7321 taaagcctca gcgggttgtg tggcggcagc gcgccatgaa gggtaaagcg tagacagcag
     7381 cgcgatagcc atcgccacca gcgcaataac aatgacctgt aaaggttcga tagccaccgg
     7441 cagcgccgcg ccatcaagca ggacgccgat tatcggcatc agattattta actggctggc
     7501 aagcagtgcg ccaagcgccg ctccgaggat cgcaccgata atcccggcgc tggccccttg
     7561 caccataaag accatcatga tttgtcgcgg agttaagcct tgcgtttgca ggatcgctac
     7621 ttcgccctgc ttctccatca ccatcagccc cagcgaggta ataatgttaa acgccgcaac
     7681 ggcgacaatc aggctcagca gtaagcccat catatttttt tccatgcgta cggcctggaa
     7741 cagctcgcct ttacgatcac gccagtcctg ccatttgctg ccttcaggca gtttttgctg
     7801 acttaaagag tcaactttca gcggctcatc cagccacaaa cgccagccgg taatattgcc
     7861 tgccggataa cgcatcagac gcgaggcatc ctcaatattc accagcattt catagccatc
     7921 gacttcactg ttagcggcaa aagtaccaat cacattgaac aggcgctgac ttggaatacg
     7981 ccccatcggc gtgaactggc tggcagatgg caccatcacg cggatttgat caccgcgatt
     8041 aacgcctagc tgtgaggcaa gttgttcgcc gaggatgaca ttatatttcc ccggctcgag
     8101 gtcagtttgt ttcacattga ccagatacgg cgttagtgga tctttttgcg ccggatcgat
     8161 acccagcatc accccgaccg ccacgctgcg cgcgctttgc agtaccacat caccggtagt
     8221 aataggtgcg acgcgattaa cgccgtccag tttgactgcc gtttccggga gttgctgcgg
     8281 gttaagagag ccatgctcag aagagagaat tgcctgtggc atcaggccaa ggatgttgtt
     8341 ttgcagctcg cgctcaaagc cgttcatcac tgacaatact gtgaccagcg ccatcacccc
     8401 gagggtaatg ccgatggtag aaagccagga gacgaaacga ccgaagcgat ccgctgcacg
     8461 cccacgcatg taacgcaggc caataaatag agcgacaggt tggtacatga aatccgtctg
     8521 gttgctgtag caaagacacg agtatataag cgaaagcaag atgggtaaac gtctgaactg
     8581 taagtgatcg tcagggctaa tctgaaaagc ccccgtaaat caatcggcta aattgccatg
     8641 ttttatcatc aatcttgccg cctgatctta atctgaaaaa tcatccaata cagacagata
     8701 cccattacac ctcgggtcat aacccgcagg atcaaccgca tgtaaattaa aaaagagcgt
     8761 gttgtcctga tgaaacaaaa agagctatgg attaaccaga tcaaagggtt atgtatttgt
     8821 ctggtggtga tttatcactc ggtcattacc ttttatccgc atatgaccac ttttcagcat
     8881 ccgttatcgg aagtcctgag caaatgctgg atctatttca atctttacct tgcacccttt
     8941 cgtatgccgg tttttttctt tatatccggc tatttgattc gccgctatat cgacagcgta
     9001 ccgtggggaa attgtctcga taaacggatc tggaacatct tctgggtgct ggcgctttgg
     9061 ggcgtggtgc agtggctggc gttaagtgcg ctgaaccagt ggctggcacc tgagcgcgat
     9121 ttaagcaatg cctccaatgc cgcgtatgcc gattccaccg gtgagttcct gcacgggatg
     9181 atcaccgcca gcaccagctt gtggtatctg tatgcgttaa ttgtctattt cgtggtatgt
     9241 aaaattttta gccgtctggc gctgccacta ttcgccttgt ttgtactgct gagtgtggcg
     9301 gttaatttcg ttcccacgcc gtggtgggga atgaacagtg tgatccgcaa tttgccttat
     9361 tacagccttg gtgcatggtt tggcgcaaca ataatgacct gtgttaaaga ggtaccgttg
     9421 cgccgccatc tgctgatggc ttctttgctg gctgctctgg cggtcggtgc ctggttgttt
     9481 actatctcgc tgctgttgtc gctggtgtcg attgtggtga tcatgaagtt gttttatcag
     9541 tatgaacaac gttttggcat gcgttccacc agtctgctga atgtgattgg ctccaacact
     9601 attgctatct acactaccca tagaattctg gtagagatat tcagcttaac tctgcttgcg
     9661 caaatgaacg cagcacgctg gtcgccgcaa gtcgaactga cactcctgct ggtttacccc
     9721 tttgttagtt tgttcatctg tactgttgcg ggcttgctgg taagaaaact ttcacagcga
     9781 gcattcagcg atctgttgtt ctccccgcct tcactgcccg ttgccgtcag ttactcccgc
     9841 taagccatcg ccccagccgg ggcgattcaa tttgctaaac catgtcgatt acggataatg
     9901 aacagcattg cttatcagag atatgccccc atatgttgag gcatatccta acgagaatct
     9961 gacaaccgtt atgcctgaac aatatcgtta tacgctgccc gtcaaagcgg gtgagcagcg
    10021 tctgctgggc gagttaaccg gcgctgcctg cgcgacgctg gtagcggaaa ttgccgaacg
    10081 tcacgccgga ccggtggtgc ttattgcgcc agatatgcaa aatgccctgc gtttgcatga
    10141 tgaaattagc cagtttaccg accagatggt gatgaatctg gcggactggg aaaccctccc
    10201 ttacgacagt ttttcgcctc atcaggacat tatctcctcg cgcctttcca ccctttacca
    10261 gctaccgaca atgcagcgtg gcgtactgat tgttccggtg aatacactta tgcagcgcgt
    10321 ttgcccgcac agttttctcc acggtcatgc gctggtgatg gaaaaaggtc agcgcctgtc
    10381 acgagatgca ttacgaaccc aactggatag cgccggttat cgccatgttg accaggtgat
    10441 ggagcacggc gaatacgcca cgcgcggcgc gttgctggat ctcttcccga tggggagtga
    10501 gttgccttat cgtcttgatt tctttgatga tgaaatcgac agtctgcggg tgtttgacgt
    10561 cgacagtcag cgcacgctgg aggaagtaga agcgatcaac ctgctgcctg cgcacgaatt
    10621 tccgaccgat aaagcggcga ttgaactgtt ccgcagccag tggcgcgata ccttcgaagt
    10681 gaagcgcgat ccggaacata tttaccagca agtgagtaaa ggcacattac ctgccgggat
    10741 cgagtactgg cagccattgt tcttcagcga accactgccg ccgctgttca gttatttccc
    10801 tgccaatacc ttgctggtga atactggcga tctggaaaac agtgccgaac gtttccaggc
    10861 tgacacgctg gcgcgttttg agaatcgcgg cgtcgatccg atgcgcccac tgttgccacc
    10921 acaatcgctc tggctgcggg tggacgagct cttctcagag ctgaaaaact ggccccgagt
    10981 gcagctaaaa actgaacatt taccgacaaa agccgcgaat gccaatttag gtttccagaa
    11041 actgccagac ctggccgttc aggcacaaca aaaagcgccg ctggatgcgc tgcgtaagtt
    11101 cctcgagtct ttcgacggtc cggtggtgtt ctcggtagaa agtgaaggcc gccgtgaagc
    11161 gctgggtgaa ctgctcgcgc gaattaaaat tgctccgcaa cgcattatgc gtcttgatga
    11221 agccagcgac cgtgggcgtt atctgatgat tggcgctgca gagcatggtt ttgtcgatac
    11281 ggtacgtaat ctggcgctga tttgcgaaag cgatctgctc ggtgaacgcg ttgcccgtcg
    11341 tcgtcaggat tctcgccgca ccatcaaccc cgatacactg atccgtaacc tcgcggaact
    11401 gcatattggt cagccggtgg tccatctgga gcacggcgtc gggcgctatg ccggaatgac
    11461 cacgctggaa gcaggcggca ttaccggcga gtatctgatg ctcacctatg ccaacgacgc
    11521 caaattgtat gttccggtgt cgtcgctgca tctgattagc cgttacgcgg gtggtgcgga
    11581 agaaaatgcc ccgctgcata aacttggcgg cgatgcgtgg tcacgcgcgc ggcagaaagc
    11641 ggcggaaaaa gtgcgtgatg tggcggcgga attgctggat atctacgcgc aacgcgccgc
    11701 caaagagggc ttcgcgttta aacacgatcg tgagcagtat cagttgttct gcgacagctt
    11761 cccgtttgaa accacgccgg atcaggcgca ggccattaat gcggtactta gcgacatgtg
    11821 tcagccgctg gcaatggatc gtctggtgtg cggcgatgtt ggctttggta aaacagaagt
    11881 ggcgatgcgc gccgctttcc tggcagtaga taaccacaag caggtggcgg tgctggtgcc
    11941 taccaccctt ctcgcgcagc agcattacga caacttccgc gaccgtttcg ccaactggcc
    12001 ggtacgtatc gaaatgctct cccgtttccg cagcgccaaa gagcagacgc aaatccttgc
    12061 ggaagtggcg gaagggaaaa tcgatattct gatcggtacg cacaaactgc tgcaaagtga
    12121 cgtcaagttt aaagatttag gcctgctgat tgtcgatgaa gaacaccgct tcggggtgcg
    12181 tcataaagag cgcattaaag cgatgcgcgc gaacgtggat attctgacgc ttactgcaac
    12241 gccgatccca cgtacgctga atatggcaat gagcggaatg cgtgacctat cgattatcgc
    12301 cacgccgccc gcccgtcgtc tggcagttaa aacctttgtc cgtgagtatg acagcctggt
    12361 ggtccgcgag gcgatcctgc gtgaaatttt gcgcggagga caggtttatt atctctacaa
    12421 tgatgtggaa aacattcaga aagccgccga acggctggca gaactggtgc cagaagcgcg
    12481 gatcgccatc ggtcacgggc agatgcgcga gcgcgaactg gaacgggtga tgaatgattt
    12541 ccatcatcaa cgtttcaacg tgctggtttg tacaaccatt atcgaaaccg ggatcgacat
    12601 cccgacagcc aacaccatta tcattgaacg cgcggatcac ttcggtctgg cgcaactgca
    12661 ccagttacgc ggtcgcgtcg gacgttcgca tcatcaggca tatgcatggc tgctgacgcc
    12721 gcatccaaaa gcgatgacta ccgatgcaca aaaacgtctt gaagcgattg cctcgctgga
    12781 agatctcggg gcaggttttg cgctggcaac gcacgatctg gagattcgcg gcgcgggtga
    12841 actgcttggc gaagaacaaa gcggctcaat ggaaaccatc ggtttctcgc tgtatatgga
    12901 gttgctggaa aacgccgtcg atgcactgaa agccggacgc gagccgtcgc tggaagatct
    12961 caccagccag caaacagaag tcgagctgcg gatgccgtcg ctattgccag atgatttcat
    13021 tcctgacgtg aatacgcgtc tgtcgttcta taaacgtatt gccagcgcca aaacggaaaa
    13081 cgaactggaa gagatcaaag tcgagcttat cgatcgcttc ggcctgctgc cggatccggc
    13141 gcgtaccctg ctggatattg cccgtctacg ccagcaagcg cagaaactgg ggatcaggaa
    13201 gctggaaggt aatgagaaag gcggcgtgat cgaatttgcc gagaagaatc acgttaatcc
    13261 ggcctggttg attggtttgc tgcaaaaaca gccgcagcat taccgccttg atggtccgac
    13321 gcgcctgaaa tttattcagg atttgagtga gcggaaaacg cgtatcgaat gggtacgcca
    13381 gtttatgcgt gaactggaag aaaacgcgat cgcttaatgg taaatcgggg gcgtttctgc
    13441 gcccccatct gcaacattta caaattcttt gcacttccct gcactatccg acactgtcac
    13501 catccataat tcaggcttat ctgtttatta caataacctt atatttatta tggatttttg
    13561 gtgatgatca aaacgcgttt ttctcgctgg ctaacgtttt ttacgttcgc cgctgccgtg
    13621 gcgctggcgc taccggcaaa agccaacacc tggccgctgc cgccagcggg cagtcgtctg
    13681 gttggcgaaa acaaatttca tgtggtggaa aatgacggtg gttctctgga agccatcgcc
    13741 aaaaaatata acgtcggctt tctcgctcta ttacaggcta accccggcgt tgatccttac
    13801 gtaccgcgcg cgggcagcgt gttaacgctc ccgttgcaaa ccctacttcc agacgcgccg
    13861 cgcgaaggca ttgtgatcaa cattgcggag cttcgtctct attactaccc gccgggtaaa
    13921 aattcggtaa ccgtgtatcc aataggtatt ggtcagttag gtggtgacac gctgacaccg
    13981 acaatggtga ccaccgtttc tgacaagcgt gcaaacccaa cctggacgcc aacggcaaac
    14041 attcgcgcgc ggtataaggc acaaggaatt gagttgcctg cggtagtgcc ggctggaccg
    14101 gataatccaa tgggccatca tgcgattcgt ctggcggcct atggcggcgt ttatttgctt
    14161 cacggtacga acgccgattt cggcattggc atgcgggtaa gttctggctg tattcgtctg
    14221 cgggatgacg atatcaaaac actctttagc caggtcaccc caggcaccaa agtgaatatc
    14281 atcaacactc cgataaaagt ctctgccgaa ccaaacggtg cgcgtctggt tgaagtacat
    14341 cagccgctgt cagagaagat tgatgacgat ccgcagctgc tgccaattac gctgaatagc
    14401 gcaatgcaat catttaaaga tgcagcacaa actgacgctg aagtgatgca acatgtgatg
    14461 gatgtccgtt ccgggatgcc ggtggatgtc cgccgtcatc aagtgagccc acaaacgctg
    14521 taaatcctgg ataaaaaaaa tccccgcgac acggcatgtc gcggggaaaa aatcagcagt
    14581 agcaggcatt aatgagggtt aatgcttatt tataaattac tgctgttcca tggagggtat
    14641 tcggaccggt tacagaagta atacggaaag attttgcgcc catctcatcc gctttttgcg
    14701 ccagctgctc ttccagcgat cccagatttg tccccgcgtt agcactgatt gtaccgactt
    14761 tttgttggcc ttctggcgtt gactgaactt cgacagccgc aaagctggca aatgacatgg
    14821 agcttaaaat cgccgcagcg atgagggttt ttacgttttt cataatagtg gccttatgca
    14881 gatgaatgac gtcgcaagca attacttaac gatcgataac taaatgatag atgtgatctg
    14941 gatcacatac aagatatttt ttataacaat cattaattaa tttaaaaatc tttaaatttc
    15001 agttaaatag aaagcaatta tttttatggg atctggactg gtgaacggag gtgattattt
    15061 ttataataac ctttcattca cagtcaccag gtacaacggc atcatggcaa ctgactcaac
    15121 acaatgtgta aaaaaaagcc gtggccgccc aaaagtgttc gacagggatg ccgcgcttga
    15181 taaggccatg aaattgttct ggcaacacgg ttatgaagcg acttctcttg cggacctcgt
    15241 cgaagcgacc ggagccaaag cgcccacgct atacgcggaa tttaccaaca aagaggggtt
    15301 atttcgtgcc gttctcgacc gctatatcga tcgttttgcc gctaagcatg aagcacagct
    15361 gttttgtgaa gagaaaagcg tggagtctgc gctggctgac tattttgctg ccatcgccaa
    15421 ctgctttacc agcaaagaca ccccggcagg ctgcttcatg atcaacaact gcaccaccct
    15481 ctccccagat tcaggagata tcgccaatac gttgaaatca cgccatgcga tgcaagagcg
    15541 cactttgcag cagtttttat gtcaacgaca agcgcgcggg gaaatcccga cccactgtga
    15601 cgtgacacat ctggcagaat tccttaattg tattattcag gggatgtcga tcagcgcacg
    15661 cgaaggtgca tcgttggaaa aactgatgca gattgccgga acgactttgc gtttatggcc
    15721 cgaactggtg aaataagtcg caaaaaaagc ccctcagcct gagcgaatga ggggccaaat
    15781 tgcagagtac agccttgtta tactttgtta ttcaaaatca gttgcccgtt actatctagc
    15841 gggatctgcg tacccggatc gcggtccatg cggattttgc cctgctgatc gccaatctta
    15901 taggtcacat cataaccgag cattttttct gacttgtcat acaccgtttt acaacgctgt
    15961 tgcgtagtcg tgtaagtatc gctttcctgg agagagccct ggatctggtt accggcatat
    16021 ccaccaccca gcgcccccac aacagtggcg acatctttac cacgaccacc accaaactga
    16081 tgccctatca cgccgccagc aacagcgccg agcaccgacc cggtaatgcg atgttcatcc
    16141 tgcaccggtc gacgatgggt cactgtgacg ttgcgacact cctgacgcgg tgttttaacc
    16201 gtttccttga ttggggttgc agaaacaacc tgagcgtatt gcgggccacg ttcaaacacg
    16261 ttcagactgg ccactgccgc tacgccaagc gcagctgcga caccaatccc gatacccgcc
    16321 aacattgatt tattcacggg aatacctcct tctttgcagt tatgccaatc atgcaagcga
    16381 aggagaaaag cagctatcag acagaagatg aaaagcgcgg gaatggctgg aaatacttaa
    16441 ggattcggag gagtctgaag gaatgcctga tgcgacgctt aacgcgtctt atcaggccta
    16501 ccgctcgtac catttgtcca gggcggatga ggcgtttatg ccacatccgc cagtgtacgt
    16561 cgattaatgc aacttcaaac gcggacggat aacgcggtta atactcccca ccagcatcat
    16621 taatccggtt ttaaagtaac catgcagcgc aatctgatgc attcggtata gcgagatata
    16681 tacaaagcgc gcaattcgtc cttcaatcat cattgagccg cgcgtcaggt tacccatcag
    16741 gctaccaacg gtggagaagt tcgacagcga taccagcgaa ccatgatctt tatactgata
    16801 atttttcagc agcttaccgt tcatctgcgc cagaatgttg ttcattgcgc aagtcgccat
    16861 ctggtgtgca gcctgagcac gcggcggaac aaagccccct tccggacgcg ggcatgacgc
    16921 gcagtcgcca atagcgtaaa tgtctggatc gcgggtggtt tgcagcgtca gttccaccac
    16981 cagctggttg atacggttag tttcaagacc accgatatct ttcaggaagt ctggcgcttt
    17041 gatcccggct gcccatacca tcagatcagc ctcaatatat tcgccatctt tagtgtgcag
    17101 gccgccttca tcagcactgg tgaccatggt ttgcgtcaga acgcgaacgc caagtttcgt
    17161 tagctcgttg tgggccgcag cagagatacg tggcggtaat gcaggcaaaa tacgttctcc
    17221 cgcttctacc agcgttacgt tcagggcttc gttggtcagg cctttgtaac cgtagctgtg
    17281 cagttgcttg accgcgttgt gcaattcagc ggagagttct acacccgtcg cgccgccgcc
    17341 gacaatcgca atgttcactt tgccatttgc gcccaggttg gcggagtatt tcaggaagag
    17401 attcagcatc tcctggtgaa agcgacgcgc ctggtgcggg ttatcgagga aaatgcagtt
    17461 ctctttgaca cctggtgtat tgaaatcgtt agaggtgcta cccagcgcca ttaccagggt
    17521 gtcataggcg attttacgtt ccggaaccag cagttcacct ttctcatcgc gcagttctgc
    17581 aatagtgatt gttttcgcgt cacgatcaat atcaatgacg gaacccagct ggaactggaa
    17641 accatgattg cgcgcatggg ccagatagct caacgcatcg acgccttcat caagcgagcc
    17701 agtcgctact tcgtgcagca gcggtttcca caggtggctg tggttacgat cgaccagcgt
    17761 aattttggct tttttcttgc gtcccagctt atgccccagc tgtgttgcca tttccagccc
    17821 accagcaccg ccgccgacaa tcacaatttt tttcaatggc gtagtcaacg tgaccccctt
    17881 aaatttatta accaattgtt aattaatggt tattaacata gctcttaatt aacaacaggt
    17941 tacagcaatg aaaatgttct tcgctattga gaataacatg aatggtgcat tggtcatacc
    18001 aaaattgatg ttaatcaagt tttgttgctg aaaagatagg cgactttacc gggtgagatc
    18061 aaaaaacaga gtttattgtt tattatttga gcgaatttgt gagagtgata tagcgtgagg
    18121 ggtgtatgta gggcgttcgg taggtctgat aagaggcgca tcaggcattg atgctcgact
    18181 gtcggatgcg gcgtgaacgc cttatccgac ttacagaatt cggagcgcat caggtaccgg
    18241 caaggttacc tgatgcgacg agggcattta cccgagggtt ttgaacgctt taatgcgctg
    18301 caaatgcggg gagatattct tgaatttgtg cgtctgctct tcgtcccaga caatctcgta
    18361 ataatgatgc aactcttcag atgtccgctg gctgttaagc gcttcatcat tacgcgacaa
    18421 aatcaccagg caacgatcgc gattcttctc gcggaagttg gtcacacact tagtggcaat
    18481 atcggcatac tcttccgggc gatcaatctt gccttccatg ttctcataag ggaacaaatt
    18541 agggttgaag atcacctggc ggatatcgca aagaaaacca atccgttccg cccagtatcc
    18601 gcccagacca acgccgcaaa tcaggggacg ctcgtcgacg ttcagttgca acattttgtc
    18661 cacttctttg agcagatgct gcatatcatg tttcggatgc cgcgtactgt agcttatcaa
    18721 gcgtacatcc gggtcaataa actgcaattg taagactttc tcgtggttac ccggactgtt
    18781 agagtcaaaa ccgtgtaaat agataatcat cgcttcctca cataagccag ggttagtgac
    18841 ctgctttctc ttcctgccag cgttcatgta actgattcag acgggcgctg atcgctttcc
    18901 agcgagccga gtccatcagt tcctgtcgcg aaaatgaacc tttatgatac aaacgtgtaa
    18961 cacgttctgc cttgatcggt gacagattat ctaacacgct gacggcccct ttacgattat
    19021 tgcagaccag gatcatatcg caacccgcat ccagtgatac ctgcccgcgt tcggcataac
    19081 tgcccataat cgcggcacct tccatcgata aatcgtcaga gaaaatcacg ccgtcaaaac
    19141 ccagttcctg acgcaaaacg gttttcagcc agtaggaaga accgctcgcc ggacgcggat
    19201 caacatcact gtagatcaca tgcgcaggca taatggcgtc gagtttattt tcgcggatta
    19261 acgtgctgaa aaccgacata tctttggcac gaatttccgc ttgcgggcgt gggtcgcacg
    19321 gcgtctcttt gtgtgaatct gcagttactg caccgtgtcc tgggaagtgt ttcccggtcg
    19381 ttttcattcc ggcttcatgc atcccatcaa taaaccgact ggcgattgcc agggcttttt
    19441 ctgggtcggc atgataagaa cgctcgccaa tcgccgcgct gatatgtcct acatccagca
    19501 ctggcgcaaa gctgatatca atatccatag cgatcatttc gctggccatc agccaacccg
    19561 cctcttgcgc cagtttgccg ccctcttcca ttcccaacag cgcagcaaag gattgtgctg
    19621 ccggtaagcg ggtaaaacct tcgcgaaaac gctggacgcg tccaccttcc tgatctaccg
    19681 ccaccaccag atgattgcgc gatgctgcgc ggatctggcg caccagttca cgcaactggg
    19741 caggatcatg atagtttcgc gtaaagagaa tcagccctcc caccagcgga tgcgccagta
    19801 tttcacgctc ttccgcgtcc agttcgtaac cttcgacatc caacattact ggacccacac
    19861 tgctctcctt attgttttat taatagctgc cgccaggtgt catcggccag cctgataaat
    19921 tgttgatcgc cggtttgtcg ccagcggtac tcaaaccacc ctgctttgag catcagcagc
    19981 cagggaaacc atcgcctgac ctgacgccat aattgcgccg gataaatctt cgcgcgagtg
    20041 gcatagtcat tgaccaattg ccggtgctgt tcagtatttt ccacccacac cgccgccagt
    20101 tccagcgcga tatcaccatc tcctgcatac tcccagtcga tgagttttaa ccctgacgcg
    20161 ctatgcacta aatttccggc gtggacatcc atatgcaatg gacttaagcg taaaggccgt
    20221 ggttcccgcg ctttgcgcag acgttttaac attcgcagcc aacccactgt ccgccgcgcc
    20281 ggatcgcttt gctgccagta cagttccagt aacggcaaca gcgttattcg ccagccaaaa
    20341 cgtggttgtt gatgtagata atacagcaag cctgccagtt cgttggtatc cggcaaatac
    20401 gtttttacct cgccgggcag atagtcgact accatccagt cacggagata taaatgcggc
    20461 ttcggtgcaa tgcatgcggg tagttgtgat aaagcccgat actggcgcaa gaacgcggac
    20521 tgcggcgcat caggatcgtg cggctgacgc acaacaaaac gctggccctg atgttcaatg
    20581 agaaaactac cgccactaag cccactatta aacgtcgtga cgggatgaaa ctgcgggaaa
    20641 aagcgcgaca gcaattcgtc gcgcgtgatg ggattattgc tgcgaaacgg cacctttacc
    20701 tgaccagata atttcgcccg tctgcaccag catcagctgc atttgtaggg tcggagcgtt
    20761 aacgttgcca gaggcgctgg agtacagcac gtaatgagcg ccgacattgc gggcaatgcc
    20821 tatggcttta ctacgggtgc ctaaactatc ctgcggcgac aaacctaact gttgcttagc
    20881 catcgacagc tgctgggcgg aaaccagggt aaatttcccg ttattagcca gcgcatttcg
    20941 cagcgtttcg gtcgcttctg cggcattcag cgaaccgtta gtacggttgt taacgctatc
    21001 aaccagcagg acgctacccg cagtcacccc gtcagcccca agcatcttac tgaccatcgg
    21061 ctgcattgcg ccattccagt cataatggcg aatatgcggc gcaggcggtg cagtttgatc
    21121 ttcgtgctca attgggcctg gctgctgcgg gatcgtcggc accgagggca ctgtggggac
    21181 aggctgttgt ggctcggctg gttgttccgg cgctggtttc acttcttcta ccggtgcagg
    21241 ttcacgttgc cccacacacc cggcgagaaa catcgccagc gcggtaatca aggcgtagcg
    21301 actcattttt gtcatcaaga ttcacccctt acaaatatag ataaagtctg actttgtgcg
    21361 cccccagaaa attggcgctg ccgtacagcg ttaccgccga atgtgcggga atggtaacgc
    21421 tgcgtggcct ttccagagga tgcatctcca gccctctggc gtcataccag taaaaacgat
    21481 aatgaacggt aacgggttct tgcctttcgt tataaagcgt tgaggatgct gaaggttgaa
    21541 tatcagacgt cgaaaggacg ggcttttctg cactgatgcc cgcagccaat aaagatgact
    21601 ccatcaccag cgattgctca tcattcaccg gaatttccgg atgtgaacga cagcccacca
    21661 gcagtaacaa cgccagagac accagcccaa agcatccttt tctcatcgtt acagaccttt
    21721 atgcgccagc attggtccca gcggacggcc acccaacaag tgcatatgga tgtggtaaac
    21781 ctcttgtccg ccatggcggt tggtgttcat gatcagacga tagccatctt cggcaatacc
    21841 ttcttgctca gcaatttttg ccgctacggt gatcatgcgt cccagcgcct gctcatgctc
    21901 agctgagacg tcgttcacag tcggaatgag gatattcgga atgatcagaa tatgcgttgg
    21961 cgcttgcggc gaaatatcgc gaaacgccgt taccagatca tcctggtaga cgatatcgga
    22021 ggggatctca cgacgaataa ttttgctgaa tatagtttct tctgccacga cattttcctt
    22081 tttcaaatag ccatgcgttg cgccatgcta cgccgcgcca cactgcgagt atagagtatg
    22141 gtcgagttaa acctgctctt tcaaccttaa ctcacgattt cttaagcaaa aaatcgtttt
    22201 gctgccgtat atatctccat tattcccatt tctgcctgcg aatgcgctga aaagacattc
    22261 tctgtttggt ttacacgaca aatatgaatg cgtatatttc tcatttgcat ttgcatttgc
    22321 aagcaaaatt attcgcacac taaatataac cgctcatcac cctcctccgg catgagcctg
    22381 acgacaacat aaaccaagag atttcaaatg ctttcaacac aatttaacag ggataatcaa
    22441 tatcaagcca tcaccaaacc gtcactactt gccggttgca tagcactggc actattacct
    22501 tctgccgctt ttgctgcacc agccactgaa gaaacggtga ttgttgaggg ttcagccaca
    22561 gctccagatg atggcgaaaa tgattacagc gtaacgtcta cctctgcggg taccaaaatg
    22621 cagatgactc aacgtgatat tcctcagtcg gtcactattg ttagccagca gcggatggaa
    22681 gatcagcagt tacaaacgct gggcgaagtg atggaaaaca cgctggggat cagcaaaagt
    22741 caggcggatt ccgatcgtgc tctttattat tcccgcggat tccagatcga taactatatg
    22801 gttgatggta tccccaccta ttttgaatcg cgctggaatc tgggcgacgc actttctgat
    22861 atggcactgt ttgaacgcgt agaagtagtg cgtggcgcga caggactcat gaccgggacg
    22921 ggtaatccat ctgcggcaat taatatggtt cgaaaacacg cgaccagtcg tgaatttaaa
    22981 ggcgatgtct cggcggaata cggtagctgg aacaaagaac ggtatgtggc ggatttacaa
    23041 agcccactca ccgaagacgg taaaatccgc gcgcgaattg tcggcggcta ccagaataac
    23101 gactcatggc tggaccgcta caatagtgaa aagaccttct tctccggcat tgtcgatgct
    23161 gatttaggcg atcttacgac gctttcagcc ggttacgaat atcagcgcat tgatgttaat
    23221 agcccaacct ggggcggttt accgcgctgg aatactgatg gcagcagcaa cagttacgat
    23281 cgcgcacgca gtaccgcacc tgactgggcg tacaacgata aagagatcaa caaggtcttt
    23341 atgaccctga agcagcggtt tgctgatacc tggcaagcga cactgaatgc cacccactct
    23401 gaagtcgaat ttgacagcaa aatgatgtat gtcgatgcct atgtaaacaa agcggatggt
    23461 atgctggttg ggccatacag taattatgga cctggctttg attatgtcgg cggcaccggt
    23521 tggaacagtg gcaaacgtaa agttgatgcg ctggatttgt tcgctgacgg tagttatgaa
    23581 ttgtttggtc gtcagcacaa tctaatgttt ggtggcagtt acagcaaaca aaacaatcgt
    23641 tacttcagtt catgggccaa catcttcccg gatgaaattg gcagtttcta caactttaat
    23701 ggcaatttcc cacaaaccga ctggtcacca cagagcctgg cgcaggacga taccacacat
    23761 atgaaatcgt tatatgctgc cactcgtgtc acccttgccg atccgctgca tctgatcctc
    23821 ggcgcacgtt ataccaactg gcgggttgat acgctgactt acagcatgga gaaaaaccac
    23881 accacgcctt acgctggtct ggtgtttgac atcaatgaca actggtcgac ctacgccagc
    23941 tatacctcta ttttccagcc gcaaaatgat cgtgacagtt caggcaaata tctggctcca
    24001 atcaccggta acaactacga gctgggtctg aaatcggact ggatgaatag ccgtctgacc
    24061 accacgttag ccatcttccg tattgagcag gataatgtcg ctcagtccac cggtacacct
    24121 atccccggca gcaacggcga aaccgcctat aaagcggtgg atgggacagt cagtaaaggg
    24181 gtggaatttg aactcaacgg cgcaattacc gacaactggc agctgacatt tggcgcaacg
    24241 cgctatattg cagaggataa cgaaggaaac gccgttaatc ctaatctgcc acgcaccacg
    24301 gttaaaatgt tcaccagcta tcggttgcct gtcatgccag agttgacagt cggcggtggt
    24361 gttaactggc aaaatcgcgt gtataccgac accgtgacac cgtatggcac cttccgcgcc
    24421 gagcaaggta gctacgcgtt ggtggatctc ttcacccgct accaggtgac gaaaaacttc
    24481 tcgttacagg ggaacgtcaa taacctgttc gacaaaacct acgataccaa cgtggaaggt
    24541 tctatcgtct acggcgcacc gcgtaatttc agcattaccg gcacgtatca attctgaata
    24601 acacctgtaa aaaaggcagc catctggctg ccttagtctc cccaacgtct tacggattag
    24661 tggttacgga tgtactcatc catctcggtt ttcaggttat cggatttagt accgaaaatc
    24721 gcctgaacac cagaaccagc aaccactacg cccgctgcgc ccagtttctt caggccagcc
    24781 tgatccactt tagacacatc agcaacgctg acgcgcagac gggtaataca tgcgtcgagg
    24841 ttagtaatgt tttctttacc accaaatgca gcaaccagag ccggtgccat ttcgctggta
    24901 cctgtcgctt ttgcatcttc agtcgcgtct tcacgacccg gcgttttcag atccagtgct
    24961 ttaatcagca cgcggaagat ggtgtagtaa acaatcgcat aaccgatacc gacgatcggg
    25021 aacagccaca gtttgctgct gttaccagac agaacgatga agtcgatcag accgtgcgaa
    25081 aacgacgtac cgtcacgcat ccccagaaga atacagattg ggaatgccag gcctgccaga
    25141 atcgcgtgga tgatgtacag gatcggcgca acgaacatga aggagaactc gatcggctcg
    25201 gtgataccgg tcaggaacga ggtcagcgcc gcggagatca taataccgcc cactttcgcg
    25261 cggttttctg gtttagcaga gtgccagata gcaattgcgg cagctggcag accgtacatt
    25321 ttgaacagga agccaccaga cagtttaccc gcagtcgggt cacccgccat ataacgcgga
    25381 atgtcgccgt ggaaaacctg acctgctgcg ttggtgtatt caccaatctg catctggaaa
    25441 ggtacgttcc agatgtggtg cagaccaaac ggtaccaggc aacgttcgat gaaaccgtaa
    25501 atgccaaacg caactaccgg gttctggtaa gcagcccact gagagaaggt ctggattgca
    25561 gaaccaatcg gcggccaaat gaaggacagc acaacgccag taaagatggc agccaggcca
    25621 gaaatgatcg gcacaaagcg tttaccggca aagaagccaa gatactcagg cagcttaata
    25681 cggtagaaac ggttaaacat gtacgctgcg atcgcaccgg agataatccc tccgagtacg
    25741 ccagtatccg ccaggtgttt agaggcgatt tcttcagcag gtaaatgcag taccagtggc
    25801 gcaaccacgg ccatggtttt aaccatgatg ccataggcaa caactgcagc cagcgcggat
    25861 acgccatcgt tattggtaaa gccgagggcg acaccgatcg caaaaatcag tggcatgttt
    25921 gcaaagacgg aaccgcctgc ttctgccata acatgcgata caacggcggg cagccagctg
    25981 aaattcgcgg aaccgacgcc cagcagaata cctgcgatag gcagtacgga taccggcagc
    26041 atcagcgatt taccgacctt ttgcaggtta gcaaatgcat tcttaaacat aattgagagt
    26101 gctcctgagt atgggtgctt tttttacgtt ctcacgcgtg gcaagggggg agagcctcgc
    26161 cgtgtacagg gcatctaagc gccctttatt tattacacag agtaaaataa ttcagtgcca
    26221 atttgtttga cggctatcac gtttcaactt cagatcattg gagaagttgc acattttgac
    26281 aaaacatatg tcacaatatt tcaaacgtgg ttattcaccg cccaattttc ggcggtgaac
    26341 tttactcgtt ttagtcatta attacgagct ttaaaaaaac tcattcaacg gatggattga
    26401 aggcgggaag cgtcgatgtg aaacagacgg gcgaagttat cggtggttac ctgcgccagt
    26461 tcttcaacgg caacaccttt caacacagcc atgtattctg caacgtcacg aaccatcgca
    26521 ggttgattct cttttcctcg atgcggcacc ggcgcaaggt aaggtgagtc agtttccacc
    26581 agtaaccgat ccagggggac ataacgcgca gcatcgcgca gttgctccgc attacggaag
    26641 gtcacaatgc cggaaaagga gatgtaaaat ccgagatcca gtaatttgcc cgccgtttct
    26701 ctgtcctctg taaaacagtg tagtacgcca ccgcaatccg tcactttttc ttcgcgcaga
    26761 attgccagcg tatcggcgcg ggcgtcacgg gtatggacga taaccggctt gttcagttca
    26821 cgaccaatct ggatatgatg gatgaaggac tcttgctgac gtactttagt ttccggtgtg
    26881 taataataat ccagcccggt ttcacccagc gctacaacac cctcttctgc cgccagacgg
    26941 cgtaaatctt ctacatcgta gggatcattc tggttgagtg gatgcacgcc acaagaaaat
    27001 acgacgttgt cacgttcgcc taccagatcc cgcatgtgta aataacccgg taatgttgtg
    27061 gcgactgcca gacaaaattt cacatcgcgt gcggcggctt tcgccagaac gtcatccacg
    27121 tccttatgca aagattcata atccagacca tcgagatggc agtgtgagtc gactaaaaac
    27181 atgatgtctc tcttaaagat gaggaaccgg tagcacaacg cccggttgca ggtaatgctc
    27241 aatacgcagt aaaagatcgg tgatgagaag ctcgcggttg atgcctgtga cagacattaa
    27301 ctgttcacga atgtggcaaa catcccccag tatagcctgc aggcgcgagg gagaaagatg
    27361 gtttgccagt tcggcgacca ggcccggcac atcaacattg gtcacctgcg cagcgccatg
    27421 atggcgtttt agcgcatcca tcagcaacgt tgccagccag tgtaaacgcg ccggagcttg
    27481 ttcatgatta agggccgcca gcagcgaata ccagtcgccc gatggcacgc tatatgccaa
    27541 cgcctgacac aatgtttcac gagcctgcca gttatctccc tgaaacaacg ccagtgccgc
    27601 gccaggcgaa ccggcgctta agcgcaatgc ggcaagtaat gcatcctgtg acattgtcac
    27661 ttcgcgtgaa agccaggtca cggcgtactg ttccggcggc ggcgcaaggt aatgtaaccg
    27721 acaacgacta cgtaacgttg ccagtaaacg ttcaggctcg cgggtagcca ggaaaaacca
    27781 ggtttctgct ggtggctctt caagcgtttt cagcaatgcg ttagccgcgg cgtcggtcag
    27841 taaggcagca tcggttaccc agacaacttt cgcaccacct aagcgtgcgt gctcattcag
    27901 cttttcggtg acctcacgta ccgcatcaat gcccagcgta tttttccctt tttcgggagc
    27961 cagggtgtag taatcgggat gcgtgccagc ctgcatcaac tgacatccac gacagtgacc
    28021 gcaacttttg tggccctgcg gttgttggca gagtaaataa cggctcaggg cgtagattaa
    28081 agcatcatcg cccatgcccg gtaacgcctg aatgagtagc gcatggtgac ctcttccggc
    28141 ctgatagctg gctaccagtt tttcgaaatc aggtcgtaac catggatacc atctcatgcg
    28201 tccaactcct tcacccagcg ggtcacggta gtgcggattg catccatcac ggcctccagc
    28261 ggctgggtgg catcaatggt atgaatactt ttatcttgtg cagccagttc cagatagcgg
    28321 gcgcgggtgc gattaaagaa atcgaaagac tcttgctcaa tacgatccag ctcgccgcgc
    28381 gcacgcgcgc gttttaagcc aacttccggg gtaacatcga gatagagcgt taagtcgggg
    28441 cgaaaatccc cgagaacagc atcacgcagt gttgccagca tatgttggtc aataccacgt
    28501 cctccgccct gatacgcctg agtggagaga tcgtggcgat cgccaataac ccaggtgccg
    28561 ttagccagcg caggtttgat gaccgtttct accagttgaa cgcgcgcggc ataaaacatc
    28621 agaacttcgg ctttatcggt aatgatttca tcgcctaccg atttgatatc cagcaccagg
    28681 cttcttaact tttcggcaag ttgcgtacca ccaggttccc gagtgaaaac catgtcgcgg
    28741 atacccagtt gctcgagcgt ctcaaccacc acattacgcg cggtagtttt gcctgcgcct
    28801 tccagcccct caatgacgat atacttactg cgcatttttt tccttaagca ctttcagata
    28861 atcctgcaca gacttgttat gactggcaag attggtatta aacgtgtgac cacctttacc
    28921 atcggccaca aaatagagat acggcgtttt tgccggatgc gcagcagcct tcagcgaatc
    28981 cgcccccggc gtagctatcg cacccggcgg caaaccggta atggtatagg tgttatacgc
    29041 tgtcggcgtt tccaggtctg cacgagaaag tttgccatta taacgctctc ccatcccgta
    29101 aatcacggtt gggtcggtct gcaggcgcat accaatgcgt aaacggttga taaataccga
    29161 ggcaacctga tcgcgttcac tggcaatggc ggtttctttt tcgataattg atgccatcgt
    29221 caccagctga tttttatctt tataaggcag accgtccgca cgcccttccc aggcgctatc
    29281 gaccgctttc accattttct tgtgcgctcg cttgagtaac gcgacatcgg tggtattggc
    29341 ggtatacatc caggtgtctg gccagaacca accttcaatc cactccgggt tttccagttc
    29401 aagtgcctgc gctacggtga tgtacttatc atcgctcagc gtgtgcttga tatacggggc
    29461 ctcacgcaat tgcttgaggt agtcgctcag acgcatccct tctaccagtc gcagagggaa
    29521 ctgtgcttct ttaccgcttt ccaacaattt cagcatctcg cgcacggtca tctgcggtgt
    29581 aaagcggtaa gtcccggctt taaagtgaga aagatccggt tcgatacgca gcaaccattg
    29641 aaaaacccgt gggcgattga tgatcttatc ggcataaagc tgttcaccaa gcgccagacg
    29701 tccggtccct ggcttcaggg taaatatcgt ctcttctttg ataagcaatt tgctgtcggc
    29761 aagatagcga accttccaga cgcccacacc agcggcgata cccagtacca ccagcaataa
    29821 caagattatc aataacactt ttttcatgac taattcgggc gctcacaaag tggggctaaa
    29881 tattcatata acgttgctga cgaaaaggag acatcgccac aggcacgtac gggcatcact
    29941 ggcattaacg cattacaaat aaccatctca tctgcctgca acgcctcttc cagagaggct
    30001 tgcacttcga caagctgata agaggattgt gccagcaaac ggatacagaa ttgtcgcata
    30061 atgccgttaa cacctgcctg atccagtcgc ggcgtataaa ctacgttgcc cttccgccag
    30121 aacaaattag ctgcacagca ttccgtaacc cccccttcgc tgtcaaggac cagcgcctca
    30181 tcagcgtttg tctgctcaag atgagagcgg atcaatactt gctcaagccg attaagatgt
    30241 ttaatacctg caagatgagg attgcgcccc agccgcaccg ggcttagcgc caacgtgatc
    30301 ccttcgttac gcaaacggtc gtaatgtgca ggataagccg taacggagag aatccgcgtt
    30361 gccggtccac tgttcaatgt gctgtaccct cgcccgccac taccgcgact gatcacgact
    30421 ttcagtacac cattttgctg ttctgctgcc agcgttttca tctcttgttc aagctgaggc
    30481 cagaagtcac aggaaatcat caaccgctga caagcatcct gtagtcgctg gatatgcgcc
    30541 gataacaaac tgactttacc gtcgataact ctggcggtgg taaaacaacc atcaccaaac
    30601 tgcgttgccc gatcgcttac tgacagcgat tcctgcttat aaccgttaat taagaacata
    30661 ccggctcctt atcagagaat tgttagtgtg gcagcatgtt cactacggaa caagtcggaa
    30721 taaaagctaa gaaaaaaggc ccgcaagcgg accttttata tgggtggaaa atgacaactt
    30781 agatcttttt aaagatcaaa gaaccattag tgccaccgaa gccgaaggag ttacacagcg
    30841 tgtattccat tccgctgacc tgacgtgctt catgcggtac gaaatccaga tcgcaacctt
    30901 catccgggtt atccaggttg atggttggcg gaacagcctg atcgcgcagc gccaggatgg
    30961 agtagataga ttctactgca cccgccgcac ctaacaggtg accggtcata gatttcgtgg
    31021 agcttaccaa cacacggctt gcagcttcac cgaagatggt tttcaccgcc tgcgcttcag
    31081 ctttatcgcc agccggcgta gaagtaccgt gcgcgttaac gtagccaatc tgactcgctt
    31141 caatgcctgc atcacgcaga gcatttgcca ttgccagagc tgcgcctgcg ccattttctg
    31201 gcggtgacgt catatgataa gcatcgctgc tcataccaaa gccgacgagt tcagcgtaaa
    31261 ttttcgcacc gcgttttttc gcgtgttcgt actcttcaag taccagcata ccggcaccat
    31321 cgcccagtac gaaaccatca cgctctttat cccacgggcg gctcgccgct tgcgggttat
    31381 cattgcgggt agataatgca cgtgccgcgc caaaaccacc aacacccagc ggcgtactgg
    31441 ctttctctgc gccacctgca accatcacgt cagcatcgcc atacgcgata atacgcgcag
    31501 catggccaat gttgtgcacg ccggaagtac aggcggtcgc gatagagatg cttgggccac
    31561 gcaggccata catgatagtc agatgacctg ccaccatgtt cacaatcgtt gacggaacga
    31621 agaatgggct gatcttacgt ggaccaccgt tcatcagaga tgtgtggttt tcttcgatca
    31681 gtccgaggcc gccaatcccg gagccaattg cggcaccaat gcgggttgcg ttctcttccg
    31741 ttatttcaag gccagaatcc tgcatggcct gaacgccagc gacaattcca tattgaatga
    31801 aggcatccat cttgcgctgt tctttgcgcg agataatgtc ctcacagtta aaatccttta
    31861 ctaagccagc aaatttcgtt gcataggcgc tagtatcgaa atggtcgatt aggctgatgc
    31921 cactctgacc ggcaagcaga gctttccagg tagactctac ggtattgccg acaggagaca
    31981 acatgcccag tccggtcaca actacacgac gcttagacac gtttgtcctc cagggaggga
    32041 aaaaatgatt ctagtgggac aaaaagataa aactcaggcg gtcgaacgac cgcctggaga
    32101 tgttcactta cgcctggtgg ccgttgatgt aatcaatggc agcctgaacg gtggtgattt
    32161 tctcagcttc ttcgtccgga atctcagtat caaactcttc ttccagagcc attaccagct
    32221 caacggtgtc aagagaatcc gcgcctaggt cttcaacgaa agaagcattg ttggtaactt
    32281 cttcctgctt aacgcccagc tgttcgccga taattttctt aacgcgttct tcgatagtgc
    32341 tcatactctt aaatttccta tcaaaactcg ctttcgcgat ggttttcgta gtgtataaaa
    32401 tgttgaaaaa tttgcaacta aatcccggca ggtcttacca cgattttacg ttattttgag
    32461 gccttacccc ccaataacgc aaatcatttt gcactaatta ttactcataa ccacgcagac
    32521 tgcgcgcaac atgagcattt tgtgcaaatc gcggtcagac catgtacatc ccgccgttca
    32581 catgcaaagt ttcacccgtg atgtaagctg cttcgtcgga tgccaggaat gcaaccgcgt
    32641 tggcgatttc ctgtgcgccg ccgaggcgac ccgcaggaac ctgcaccagg atacccgcac
    32701 gctggtcatc gctcagcgca cgtgtcatgt ccgtttcaat aaagcccgga gcaacaacgt
    32761 ttacagtaat accgcgtgac gcaacttcgc gcgccagtga tttactgaag ccgatcaagc
    32821 ccgctttcgc cgcagcgtag ttggcctgac cgccatttcc catggtacca accacagaac
    32881 cgatagtgat aatacgacca tgacgctttt tcatcatagc gcgcattacc gcttttgaca
    32941 gacggaaaac agatgaaagg ttggtttcga taatatcgtt ccactcttca tctttcattc
    33001 gcattaacag gttatcacga gtgataccgg cattattgac caggatatcc acttcaccaa
    33061 attctgcgcg aattttttcc agaacagatt cgatagatgc cgggtcggtc acattcaaca
    33121 tcagaccttt gccgttagca cctaaataat cactgatcgc ctgagcgcca ttttcactgg
    33181 tcgcagtgcc aataactttc gcgccacggg ctgcgagcgt ttcagcaatt gcgcggccaa
    33241 ttccgcggct tgcaccggtt accagtgcga tttttccttc aaaattcatg atgttcctct
    33301 tttaaagctc gagcgccgct gccatcgctg aaggttcgtt cagcgccgag gcggtcaggg
    33361 tgtcgacaat gcgtttcgtc aggccagtaa gcactttgcc cgggccgact tcatagagat
    33421 gttctacgcc ttgcgctgcc atgtactcaa cagacttcgt ccactgaacc gggttataca
    33481 actgacgtac cagtgcgtca cgaatggcat caccattggt ttcgcatttc acatcaacgt
    33541 tattcacaac aggaactgtt ggtgcgttaa aggtgatttt cgctaattct actgccagtt
    33601 tgtcggctgc tggtttcatc agcgcacagt gagacggtac gctcactggt aacggcagag
    33661 cgcgttttgc gcccgcagct ttacaggcag cgccagcacg ctcaaccgct tctttatgac
    33721 cggcaataac cacctgtccc ggagagttaa agtttaccgg agaaacgacc tgaccttctg
    33781 cagcttcttc acacgctttt ccaatagacg catcatccag accgatgatt gccgccatag
    33841 cgcccgttcc ttccggtacg gcttcttgca tgaacttgcc gcgcatctca accagacgca
    33901 ccgcatcagc gaaatcaatc acaccagcgc aaaccagcgc ggagtattcc cccaggctgt
    33961 gaccggccat cattgccggt gctttaccgc catgctgctg ccatacgcgg tacagcgcaa
    34021 cagaggcagt caacagcgct ggctgggttt gccaggtttt attcagttct tcagctggcc
    34081 cctgctgggt cagcgcccac aggtcgtagc ccagcgccgc agaagcttca gcaaacgttt
    34141 cttcgacaat tggatagctc gccgccatat cagccagcat tccaacggtt tgagaaccct
    34201 gtccagggaa cacaaatgca aattgcgtca tgttttaatc cttatcctag aaacgaacca
    34261 gcgcggagcc ccaggtgaat ccaccgccaa aggcttcaag cagaaccaac tgccccggct
    34321 tgatgcgccc gtcgcgtaca gcttcatcca gcgcgcacgg gacagaggcc gcagaggtat
    34381 taccgtggcg atccagcgtc accacgacat tgtccataga cataccgagt tttttcgccg
    34441 ttgcactgat aatacgcagg ttagcctgat gcggaaccag ccagtccagt tgagaacggt
    34501 caagattatt cgccgccagc gtctcatcaa cgatgtgcgc cagttccgtt accgcaacct
    34561 tgaagacttc gttgcccgcc atcgtcagat gaattgaatt ctctggattc acgcggtcgg
    34621 cgtttggcag cgtcagcaat tcaccataac taccgtcggc atgcagatgg gtggaaatga
    34681 ttcccggctc ttcagaggca gccagcaccg cagcgcccgc gccatcgcca aaaataataa
    34741 tagtcccacg atctgttgga tcgcaggtgc gcgccagtac atcggaaccg acgaccagag
    34801 catacttcac cgccccagat ttcacgtatt gatcggctac gcttaatgca taggtgaaac
    34861 ctgcgcaggc tgctgcaacg tcaaatgccg ggcaaccttt aatgcccaac atgctttgaa
    34921 tctgacaagc tgcgctcggg aaagcgtgcg tagcagaagt cgttgccaca acgatcaggc
    34981 caatctggtc tttctcaatg cccgccatct caattgcgcg tgtcgccgct tcaaagccca
    35041 tggttgaaac ggtttcgttt ggcgcggcaa tgtggcgttc gcggataccg gtacgagtga
    35101 caatccactc gtcagaggtg tccaccattt tttccaaatc ggcgtttgtc cgcacttgtt
    35161 cgggcagata gctgccagta ccaataatct tcgtatacat gtacgctcag tcacttttcg
    35221 gttatatacc gtcacttgca aactgcgagt tcgctggcag cgtcctgcta ccgcagagtt
    35281 ccgcttttgc caccgtccag cagctcaaaa ccagctgggt atacagattc caggcgagcg
    35341 gcaattcgct gaggaacttg tcgctgcacc gcctgcactg cctgttcaat cgcgaccgca
    35401 aaagctcgct gattggctgc accatgactt tttatcaccg tgccgcgcaa tcctaacaga
    35461 caggcgccgt tatactggtc ggggttgagg tgactgaatc gcctcgtcag gctcttttgt
    35521 agccaacgct ttaataacag tagccaccac gaccgttttt tcccttcacc ctgagatttc
    35581 agcagagaaa ggaacatcct gacaacacct tccatcgtct ttaatgtgac atttcctgta
    35641 aagccgtcac aaaccagcac atctgtcttg ccagttaaca actcattggc ttcaagatag
    35701 ccgatataat tgatagaagg gattgtttta agcaccgctg aggcatcccg aatactatcg
    35761 agacccttta cttcttcttc accaatattg agcaacgcca cgcgaggatt gggaatttcc
    35821 accacctctt cagccaggac tgagcccata atggcaaatt gcaccaacat tgtgctgtca
    35881 caatcgacgt tggcccctaa gtcaaggacc accgttttgc ccttttgctg atgtggtaat
    35941 accgtcacca gcgccggacg ctcaatcccc tccaggggct tgagtaataa ttttgccagc
    36001 cccatcagtg ccccggtatt accggcactg acacaggctt gcgctcgacc ttctttcacc
    36061 agctccaggg ccacgcgcat tgaactccca cgactggcgc ggatagcttg cgaaggccgg
    36121 gcatcactgg cgataactga ctgcgcagga ataatctgca gacgcgaacg ttgttcaaag
    36181 tcagctttag caagtaatgg cgtgatggcg tcgggattgc cgactaaaag aagagtgagt
    36241 tgcgaattag aattcagtgc ctgcaatgct gcaggcactg tcacggaagg gccaaaatcc
    36301 cctcccatga catctaacgc cagggttaga cgtgtcaagg tatcgtcgcc gcccggtttg
    36361 gtctttcccc agttgcctgg ggaaaatcct cactaagctt catcacgcag acgcgtgatt
    36421 acttagcgat gaccttgcgg ccgcggtagt aaccgtcggc agtgatgtgg tgacgcaggt
    36481 gtttttcacc agaagttttg tctacagaca ggctggtgac tgcggtcagc gcgtcatggg
    36541 aacgacgcat gccacgtttg gaacgggttg gtttattctg ttgtacggcc atggacctta
    36601 ctcctcaatt acggtttatc cccgatccaa cggggagcac caattactta cgctttaagc
    36661 tggctaatac ggcaaatggg tttggctttt gcgcttcttc aggcagttca ccaaagacca
    36721 tgtccgcttc ggacacttca cagtgttcag aatcatgcac cggaactacc ggcaaggcga
    36781 ggatgatttc atcttcaacc attgcaagca gatcgatttc accgaattcg ttaacctcaa
    36841 tcggttcata cgcttccggc agtgcttcag cctgttcgtc tgaacgcaca ggactaaaac
    36901 aatacgttgt gtagacctga tgagtaaacg gcttcccgca acgctgacac tcgagcgtta
    36961 ccgtcacctt cgcatcgccg tttaacactg cgagacgttg gttatcgata gcgaacgaca
    37021 tggagcattc cacatcactg tccacactga ctacggattc ggcgacgcgc tcaacctgat
    37081 caggagtata gataccctgg taatcaaggc gtttttgagc cgtacgaacc ggatcgagag
    37141 tcaggggtaa ttttaccttt tgcatagggc gcgcatatta actttgtaac gtcatagagt
    37201 caaagaaaaa ggcagcctgt ggttgccttt tgccaataat tcgcacacat tgcgggttac
    37261 tgctttattt tcgttcagtg ctgataacga acatcaacct gcaaaacgaa gcgcatagtt
    37321 taaaatggct atcatcaata cgctatatct gatggaaaaa aatatgccta aacttatttt
    37381 agcctccacc tcgccctggc gtcgcgctct tctggaaaaa ctgcaaatct cttttgaatg
    37441 tgcagcaccc gaggtcgacg aaactccaca cagcgacgaa tcaccgcgac agttggtgct
    37501 tcgactggca caagaaaaag cgcaatctct ggcgtcacgt tatccggatc atttaattat
    37561 tggttcagac caggtatgtg ttcttgatgg cgaaatcacc ggtaaaccgt taacggaaga
    37621 aaatgcccgt ctgcaattac gcaaagccag cggcaatatc gtcactttct atactggact
    37681 ggcgttgttt aattcggcga atgggcatct gcaaacagaa gtggagcctt ttgacgtcca
    37741 tttccgtcac ctgagcgagg cggagattga taattacgtg cgtaaagagc atcccctgca
    37801 ctgcgcgggt agctttaaga gtgaaggatt tggcattact ctgtttgagc gtttagaggg
    37861 gcgtgatcct aacacgctgg ttggtttacc gctaatcgcg ctgtgtcaga tgttacgtcg
    37921 ggaagggaaa aacccgctga tgggatagtt ttttgcgccg gatgcggtta cgtcgcatcc
    37981 ggcaatcggt gctggcggcc tgatgcggcg taaacgcctt atcccattat catgcttata
    38041 tgctcgtatt agcgcgcgtt acgcagcttt tgcaaacaac gcttcaaacc atcatccatc
    38101 ggcgcttcga tacgcatcac ctcaccggtc cccggatggg taaacttcaa cgctgcggcg
    38161 tgcaggaaca gacgatttaa tcccgtgcct gcttcagtga gctgtctgtc aaattcacgg
    38221 tcaccgtagc gatcgtcaaa ggcaatcgga tgacctgcat actgtgtatg cacacggatc
    38281 tgatgagtgc gccctgttac cggactacaa cgcaccaggg tggcaaatgc atagcgttct
    38341 tccactttaa agcgtgtttc cgacggtttg ccttcctgac tcacacgcac gatacgttcg
    38401 ccgctttgca gaatattttt caataacggc gcctgaacgc tcttcacatg cgactgccac
    38461 tgaccgcgca ccagcgccag gtaatctttt tgcatccctt tttcacgtaa ttgctcatgc
    38521 agagaacgca acgccgagcg ttttttcgct accagcaaca cacctgaggt gtcccggtca
    38581 agacgatgaa ccagttcaag gaaccgcgct tccgggcgca acgcccgcaa accttcaata
    38641 acgccgaagc ttaaaccact gccgccatgt accgccgtac cggaaggttt attcagcacc
    38701 aggatgtgat catcttcata taagatgacg tccgccagcg ccgccacctt ttgcagatgt
    38761 ggcgaaaccg cctcttcttc ccgctcagca acgcgaaccg gtggaatacg cacctcatca
    38821 cccgcttcga gtttatattc aggcttaata cgttttttgt tcacccgcac ttcgccttta
    38881 cgcaaaatac ggtaaatcat actttttggt acgcctttca attgggtacg caaaaagttg
    38941 tcgatacgtt gccccgcttc gtcggcggtg atagcaacaa tttttacgga tggagtctct
    39001 gttttcatgg tgggcgattc taattagcca acaggattcg cgccactcat ttttctatgc
    39061 ttatatttag ttttgcacct tattacttca ctacgtgatc actttattga tggttattaa
    39121 accaatcacc agcaagaagt gaaaaaactg tgagtaagcg ggtgataaat ggtaaaagtc
    39181 atcttgctat aacaaggctt gcagtggaat aatgaggccg tttccgtgtc catccttgtt
    39241 aaaacaagaa attttacgga ataacccatt ttgcccgacc gatcatccac gcagcaatgg
    39301 cgtaagacgt attgatcttt caggcagtta gcgggctgcg ggttgcagtc cttaccggta
    39361 gatggaaata tttctggaga gtaataccca gtctgtttct ttgataattg cgctgttttt
    39421 ccgcatgaaa aacgggcaac cgacactctg cgcctctttg agctgacgat aaccgtgagg
    39481 ttggcgacgc gactagacac gaggccatcg gttcacaccc ggaaaggcgt tactttgccc
    39541 gcagcttagt cgtcaatgta agaataatga gtaagttacg atgaaaagaa tgttaatcaa
    39601 cgcaactcag caggaagagt tgcgcgttgc ccttgtagat gggcagcgtc tgtatgacct
    39661 ggatatcgaa agtccagggc acgagcagaa aaaggcaaac atctacaaag gtaaaatcac
    39721 ccgcattgaa ccgagtctgg aagctgcttt tgttgattac ggcgctgaac gtcacggttt
    39781 cctcccacta aaagaaattg cccgcgaata tttccctgct aactacagtg ctcatggtcg
    39841 tcccaacatt aaagatgtgt tgcgtgaagg tcaggaagtc attgttcaga tcgataaaga
    39901 agagcgcggc aacaaaggcg cggcattaac cacctttatc agtctggcgg gtagctatct
    39961 ggttctgatg ccgaacaacc cgcgcgcggg tggcatttct cgccgtatcg aaggcgacga
    40021 ccgtaccgaa ttaaaagaag cactggcaag ccttgaactg ccggaaggca tggggcttat
    40081 cgtgcgcacc gctggcgtcg gcaaatctgc tgaggcgctg caatgggatt taagcttccg
    40141 tctgaaacac tgggaagcca tcaaaaaagc cgctgaaagc cgcccggccc cgttcctgat
    40201 tcatcaggag agcaacgtaa tcgttcgcgc attccgcgat tacttacgtc aggacatcgg
    40261 cgaaatcctt atcgataacc cgaaagtgct cgaactggca cgtcagcata tcgctgcatt
    40321 aggtcgcccg gatttcagca gcaaaatcaa actgtacacc ggcgagatcc cgctgttcag
    40381 ccactaccag atcgagtcgc agatcgagtc cgccttccag cgtgaagtcc gtctgccatc
    40441 cggtggttcg attgttatcg acagcaccga agcgttaacg gccatcgaca tcaactccgc
    40501 acgcgcgacc cgcggcggcg atatcgaaga aaccgcgttt aacactaacc tcgaagctgc
    40561 cgatgagatt gctcgtcagc tgcgcctgcg tgacctcggc ggcctgattg ttatcgactt
    40621 catcgacatg acgccagtac gccaccagcg tgcggtagaa aaccgtctgc gtgaagcggt
    40681 gcgtcaggac cgtgcgcgta ttcaaatcag ccatatttct cgctttggcc tgctggaaat
    40741 gtcccgccag cgcctgagcc catcactggg tgaatccagt catcacgttt gtccgcgttg
    40801 ttctggtact ggcaccgtgc gtgacaacga atcgctgtcg ctctctattc tgcgtctgat
    40861 cgaagaagaa gcgctgaaag agaacaccca ggaagttcac gccattgttc ctgtgccaat
    40921 cgcttcttac ctgctgaatg aaaaacgttc tgcggtaaat gccattgaaa ctcgtcagga
    40981 cggtgtgcgc tgtgtaattg tgccaaacga tcagatggaa accccgcact accacgtgct
    41041 gcgcgtgcgt aaaggggaag aaaccccaac cttaagctac atgctgccga agctgcatga
    41101 agaagcgatg gcgctgccgt ctgaagaaga gttcgctgaa cgtaagcgtc cggaacaacc
    41161 tgcgctggca acctttgcca tgccggatgt gccgccagcg ccaacgccag ctgaacctgc
    41221 cgcgcctgtt gtagccccag caccgaaagc tgcaccggca acaccagcaa ctcctgcaca
    41281 acctgggctg ttgagccgct tcttcggcgc actgaaagcg ctgttcagcg gtggtgaaga
    41341 aaccaaaccg accgagcaac cagcaccgaa agcagaagcg aaaccggaac gtcaacagga
    41401 tcgtcgcaag cctcgtcaga acaaccgccg tgaccgtaat gagcgccgcg acacccgtag
    41461 tgaacgtact gaaggcagcg ataatcgcga agaaaaccgt cgtaatcgtc gccaagcaca
    41521 gcagcagact gccgagacgc gtgagggccg tcagcaggct gaggtaacgg aaaaagcgcg
    41581 taccgccgac gagcagcaag cgccgcgtcg tgaacgtagc cgccgccgta acgatgataa
    41641 acgtcaggcg caacaagaag cgaaggcgct gaatgttgaa gagcaatctg ttcaggaaac
    41701 cgaacaggaa gaacgtgtac gtccggttca gccgcgtcgt aaacagcgtc agctcaatca
    41761 gaaagtgcgt tacgagcaaa gcgtagccga agaaacggta gtcgcaccgg tagctgaaga
    41821 aactgtcgct gccgaaccaa ttgttcagga agcgccagct ccacgcacag aactggtgaa
    41881 agtcccgctg ccagtcgtag cgcaaactgc accagaacag caagaagaga acaatgctga
    41941 taaccgtgac aacggtggca tgccgcgtcg ttctcgccgc tcgcctcgtc acctgcgcgt
    42001 gagtggtcag cgtcgtcgtc gctatcgtga cgagcgttat ccaacccaat cgccaatgcc
    42061 gttgaccgta gcgtgcgcgt ctccggaact ggcctctggc aaagtctgga tccgctatcc
    42121 gattgtacgt ccacaagatg tacaggttga agagcagcgc gaacaggaag aagtgcaagt
    42181 gcagccgatg gtgactgagg tccctgtcgc cgccgctgtc gaaccggttg ttagcgcgcc
    42241 agttgttgaa gaaatggctg aagtcgtaga agcccccgta ccggttgccg aaccgcaacc
    42301 ggaagtggtt gaaacgacgc atcctgaagt gattgccgcc gcggtaactg aacagccgca
    42361 ggtgattacc gagtctgatg ttgccgtagc ccaggaagtt gcagaacacg cagaaccggt
    42421 ggttgaaccg caggaagaga cggcagatat tgaagaagtt gccgaaactg ctgaggttgt
    42481 ggttgctgaa cctgaagttg ttgctcaacc tgccgcgcca gtcgtcgctg aagtcgcagc
    42541 agaagttgaa acggtaaccg cggttaaacc agagatcacc gttgagcata accacgctac
    42601 cgcgccaatg acgcgcgctc cggcaccgga atatgttccg gaggcaccgc gtcacagtga
    42661 ctggcagcgc cctacttttg ccttcgaagg taaaggtgcc gcaggtggtc atacggcaac
    42721 gcatcatgcc tctgccgctc ctgcgcgtcc gcaacctgtt gagtaataat tagctcaagt
    42781 aatcaagccc tggtaactgc cagggctttt ttatttcatc tttgaatcaa catattaaaa
    42841 ttcatcgaat ggcatccttg ctaaccaaca atgcaaaata ggcaaaaaaa tgcccgcccc
    42901 ggcgatccct gagcgggcag acaaaacata cccagtttca tgatatgttt taaagcgagc
    42961 ttatttgttg agctggaaga gcgacaatcc ctgcatatcg gtaaatgctt tatacgatgc
    43021 ctgcaatgcc gtttgctgca tgatgtaaga tgaaatagtt gcattccagt ccacatcaac
    43081 cagatcgctc atctgctgcg tttgccctaa tgcgcgatcg ctacctaatg aatccagcga
    43141 ctccagttcg ttcagctgcg tgcctaattc cgcgcgaacg gtcaggacat tgttcagcga
    43201 gtttttcagt ccgcggttgg ttttatctaa cgccgcagcg gcggtttctt tatccgcttc
    43261 gctatccgcg accggcgttt tcagggctgc gatggcgcta tccagcatgg caaaaagatt
    43321 ggtttcagaa gcgctaccgt ctggttccgc taccgcgtta ctggtaatac tgtcgaaaat
    43381 tttgtcgccc gtatgtccta tcaccatcga acgcgaagca tcgacctgtt gcttaatact
    43441 ttctgttccg ccgacataat caccgttaac ctcactaaac ggcgcagtct ctgttttata
    43501 accggcaaaa atgtagcgcc cgttaccgtc agtggtgttc gccagattca gcaactggtc
    43561 acgaagcccc tgaatatccg tagccagcga ggcccggtca ttgtcactca aggtgccatt
    43621 gctggcgtag acaatttttt cctgagcatt ctggatagcg gtggtgacct ggctaagtac
    43681 actctcttcc agtgacactt tttgagtggc gaaagtacgt gccagcgtgt actggctgtt
    43741 ttgcgcctgt gcctgggaga gaacaacagc ttgtgatgca gcaatgggat cgtctgaagg
    43801 gttaacgact cgcttacccg tcgacatctg ctcgccgtac ttcatccatt ctgcctgaga
    43861 attggtgatg ccgcgcatgt tttgctggta catcatctgg gtactgaaac gcattttatc
    43921 tccccttagc gaatgttaat cagcgcatca aaaatcgcgt ttgccgtctg cagaacctgc
    43981 gcattcgcca ggtaatactg ctgaaaacgt tgcagatttc cgtactcctc atcgagattg
    44041 acaccggaaa tcgactgctg ctgattggaa agctgcgtca ccacattacc ttgcgtggtg
    44101 ctactggttt tcaacgtcgc ggttttatta ccgatatcac tcactaacga ggcataagcg
    44161 tcgttaaagg atttcgctcc gcccaccgtt ttactgttgc tttgcagatc cagcagggcc
    44221 tgaccgttgc ggttgtcgct atcacccgca tcttcttcgc tcgccatcgc tattttcgct
    44281 tcgtcggtga ttaatacatc catgttgacg atggcgtcac ttactggttt cagcgtgaag
    44341 ctgtcgttaa cggcaggcgt tcctgtaaac gtcaactcca gaccatcaaa tgccacttta
    44401 ccgttggcat ctggcgtcac cgtaaaagtg gtattgctgg caaggcgggt gacctgccac
    44461 tgattattat cgaacgagat tttgtaatct gtcgccagta ccgcggaggc atcagttacc
    44521 gtggcaccga tcgcaacgtt accgttgttt ttagtgtttt gcagaaccgc gggcttaccg
    44581 atagcaaaga aatcttcacc ctcatcgccg ttggcatcaa atccggcttt gtgttggctg
    44641 ttgaaagcct cagcaaatgc cagcgccagt tgtccaagcg tattacgcgt ctggtccaga
    44701 tcctgagaac ggaatgtcag aatgccgccc agcgacccgg tattcagtaa tttctccggg
    44761 atctcaatat tgcctgccgt cccatcaaca taagcgacag tcgtacgaga agggtcggcg
    44821 ctggaaggaa ctgccgccag ttgccgcgcc gtacttccct gaaccagtga gtaaccattg
    44881 gccatcgtga tgttataagt gccgccatcc tgaacgctga cttctacacc aacaatctgg
    44941 tttaattcgc tcaccagttg atcgcgttga tccagcagat tgttaggtga cgcccctgcc
    45001 cccacgcctg tcaggcggga gatttgatcg ttcaggctgg cgatttgttt agcgtaattg
    45061 ttgatctgat caacgctggc acctatcgcg atattgacct gtttgtcctg gtcgcgaaga
    45121 tactgatcgg tggttttaaa ctgattcacc aacccttctg atttcccaat cagcgcctgg
    45181 cgcgctgccg ggtcttccgc gttactcacc agcgtttgca ggctggtgaa gaaatcctgc
    45241 atctgtgttg ccagcgaaga ggtactggtg gagagcatat tgtcgatttt cgacatctgc
    45301 tcatagcggg cagtcaggcc gctactttgc gtctgcgccg cacgtaactg gttggtaata
    45361 aacgcatcat actcacgctg cacaccagaa acgtagacac cattaccaac ccagccgcca
    45421 gcgcccaacg tgctattggc ctgcgccata atagtggttt ggcgggtata tccggcaacg
    45481 ttatagctgg agatattatt acttgccgta tttaacgccg cctgggccgc gttcagtccg
    45541 ctcatggcgt tattgatcaa gctggacatg gaggttcctt ataagccttc aattacagag
    45601 tattatcggc agcgacccgc cggacttgag ttattcagaa cagattatca atgttcatgc
    45661 tgtaggtttt gctcaccttg tcgcttatcg atttcatctg ctgaatcatg ttggtgagtt
    45721 tacgggcata gtgaggatcg gtggcatagc ccgcgtcctg tagggcctgc gccccctgct
    45781 ccgcactcgc ggcggtcgtc acggcggcgt agcgcgggtt acgtgttaac agcccaacgt
    45841 aatccgacaa tgcttccaga tacgagctgt agacccgaaa cttcgctttt actttcttcg
    45901 cttcgccatt ttcatattca gtcgtggtga tttcagtgac tggccctttc cagttgccag
    45961 aggctttgac accaaacagg ttatagctcg gctcgccgtt ttcgcggcgg atttgccgtt
    46021 gcccccagcc agattccagc gccgcctgag cgaggatcaa atgatgtggc acaccgcttt
    46081 gctggcttgc cagttgggcg ggcagcgaga gttgcgcgag gaatgcttta ctgtcacctg
    46141 gcagcgaatc atcgtagtta cgtggcacgg ccttttgcac cagctgcgaa agcgcctgat
    46201 tttgataacg caccacggtt tcgagcggga atttcatcgg tgctgctggc gtggactcct
    46261 ctggcaatgg ttgttctggc gtcatctgct taaccatcat ctctgcaagc cccagacctt
    46321 tgcccgtcgt catctgttgg gcaatctgct ggtcatacat actggtatac aggcgagtgt
    46381 gctcgctgct gaacaggcca tcttttggta acgcgtcgcg catgcttttc aacatcatct
    46441 gcacgaacat cccttccacc tgacgggcca ccggacggat atttgccgcc ggatcttcgc
    46501 tcgctttcgc ctttagttcg ttgagtgatt gcgcatccca ggccgcactt gccagtagtt
    46561 tgctgtcgct gatcatcaga tgatttccag ttttgcccgc agacatcccg cactttgcat
    46621 tgattgcaaa atagacatca gatccatcgg cgtagcgccc agcgcattga gcgcgcgcac
    46681 cacgttattg aggctggcgc tggaacgtac gctttgcagc gaaccgccgc tctggcgtaa
    46741 gtcgatctgc gtttgtggcg ttaccacggt ctgtccgcca ccaaacggtg tatctggttg
    46801 gctgacattg gcctgacgat tgactgttac tgagagattt ccctgcgcta tcgcgcagct
    46861 gtcgagagtc acttcgcgat tcatcaccac cgaaccggtg cgcgagttaa tcactacttt
    46921 agcgtcctgc ggggtgacat taacctgcat attctggata tcggcaagga aacggacctg
    46981 ggaactgttg ccactcggta cgcgcacctg aatagtccgc gcatccaacg cggtggcgct
    47041 gccatatcca cgcacgcggt tgatggtgtc agcgatttgc tgcgccatgc tgaaatcttc
    47101 gtcgttaagt tgcaaattaa gggtattccc gacgccaaac tggctgggta attcacgttc
    47161 aataactgca ccattggtga tccgtccacc gttcagttgg ttcacctgaa cactgctacc
    47221 gccagcggag gctcctgcgc cgccaaccag aatattgccc tgcgccagcg catacacctg
    47281 actgtcaacg cccttaagcg gagtcatcaa caatgtgccg ccacgcaggc ttttgacatt
    47341 tcccatggaa gaaaccacca cgtcaatggt ttgcccctga cgtccaaacg gtggaagtga
    47401 cgccgtcacc attaccgcag caacgttttt tagctgcata ttggtgcccg tcggaacggt
    47461 aattcccagc tgtgagagca tgttattaag cgtttgtgtg gtaaacggcg tctgggttgt
    47521 ctggtcaccg gtgccatcca gccccaccac cagaccatag ccaatcagtg agttttgcct
    47581 taccccctga acactggtga gatcgcgaat acgctcagcc tgagccgccg tcgtaaccag
    47641 tagaagaatt aatgcagaga gaaatttaat caccacagcc tcacttacat tggcgacagg
    47701 ttaaggaaga aacgctgcaa ccagcccata ttttgcgctt cgttaatgta gccattgcct
    47761 acgtattcaa tgcgcgcatc cgccacctga gtagacggta cggtattgct gccgctgata
    47821 gtgcgtggat taaccacgcc agagaagcga ataaattcgg taccctgatt aatggcaatc
    47881 tgtttttcac ccaccacatg caggttgccg ttgaccagta cctggtcaac cgtcaccgtc
    47941 aacgtgccgc taaaggtatt actggcattg gccccgcctt ttccgttgaa cgtgttacca
    48001 ccggaggctt cgacatcggc acgagcgtta ccaaacagcc cctgcaaata gcgcggcaca
    48061 gtatcaaagc caaaattagt tttaccgtca cggctggcat tcgcagagga gcttttgctg
    48121 gcgctaacgt tctcctgcaa cacgatggtc agcgtatcgc caatattgcg tggtcgacga
    48181 tcttcaaaca gcggttgata gccatagtta atcggctgag cagactggaa aatagaaccg
    48241 ttggcgacgg gcgtcggacc gggaaccggt tgtgcactgg tcgccccctg caccagcggc
    48301 gtggagggta tccaggcgca gccggttagt gaaagcacca acaagctgga aatggcataa
    48361 gtatgcgcag cgtttttttg catggctatc atcttcaaaa atcaataaac cggtgaacct
    48421 gccaccggtt atgccttaga gttgcgtcag tttttgcagc atctgatcgg tggtggacac
    48481 cgctttactg ttgatttcgt aagcgcgttg cacctgaatc atattgacca gttcttccgc
    48541 cacgttgacg ttagacgttt caacataccc ttgatacagc agtcccgcgc cgttcaggcc
    48601 cggcgtgctt tcgttcggtg caccggagga ttgcgtttcg gtgtagaggt tttcgccaat
    48661 gctctccagc ccggtgtcat tcataaaggt ggtgagattg agctgcccaa cctgaaccgg
    48721 agctgcctgg ccttgttggg ttacgctgac cacgccatca cgaccgatgg tgatacttaa
    48781 cgcattcgcc ggaatggtga tcgctggctg cacctgaaaa ccaccagccg tcaccagctg
    48841 cccgttctga tccacctgga aagagccgtc acgggtatag gctgatgagc catctggcaa
    48901 catcacctgg aaaaagccct gccctttaat cgcgacatct ttgctgttgt tggtctgcga
    48961 caggttgcct tggctgtgta agcgttcagt ggcgaccggg cgtacccccg tgccgatttg
    49021 taatccggag ggtaaggtgg tttgttcgga agactgtgcc cccggctggc gaatggtttg
    49081 ataaagcaga tcttcaaaca ccgcgcgctg acgcttaaaa ccgttagtac tgacgtttgc
    49141 caggttgttg gcaatgacgt ccatattggt ttgctgggcg tcaaggcccg ttttggcgat
    49201 ccataatgaa ctgatcataa aatgtcctgt agtggattgc tgacaagcag agcagaagca
    49261 cgcggcggtg cgaatttccc ctcaccctaa ccctctcccc aaaggggcga gggaacagtc
    49321 cgctcgcttt tattgcaatg tcatcaatcg ttcaattaac tcatcgacag cagttggttg
    49381 gcacggcctg cgttatcatc gacgctgctg atcaccttca tctgcatttc aaaacgccgc
    49441 gcgctggcaa tcatgtcgct cattgccgca acggcattga cgttactgcc ttccagaacc
    49501 cccgacatca cacgcaaggt tggatctgcc tgcagtaccg gtccacgcgt ggcctgggtt
    49561 tctgcgctta aacgaaaaat gccgtcgtca ccgcgctgca cttcgctgcc cgtggctttc
    49621 accagtttca gacgccccac tggcgcaacc gtatttgccg gatcgcccgg attcagcgcc
    49681 gagattgttc catcggcagc tatcgtgatt tccgcccctt ccggcacagc aattggcccc
    49741 gcctcgccta tcaccggatg cccctgaatt gtcagttgcc cggtgggatc aacctgaatg
    49801 ctgccattac gcgtataccc ttcgctgccg tcagcggtct gcacggccaa ccagccatcc
    49861 tgctgcaacg caacgtccag cgggcgcgag gtgtaatcca tcttgccggg cgttatgtct
    49921 gcacccggtg ttgacgccgt taccagcgtg cgcgtgggca gtgaaagccc ttccaccggc
    49981 actgcgcgca aggcgtttaa ctgcgcgcga aaaccgggcg tagaggcatt ggccagatta
    50041 ctggcggtta ccgcctgttg attcagcgtc tggcttgctg ctcccatcgc ggtataaatt
    50101 gcgtgatcca ttgagctatc ccgtcagcga ttagcgtaag ttaaccagcg tgttgaggat
    50161 ctggtcctgg gttttgatgg tctgggcgtt agactgatag ttacgctggg caacgatcat
    50221 attgaccagt tctttactga gatcgacgtt ggacgcttcc aacgcaccgt tggtcagggt
    50281 gccaaagttg cccgtcccgg ctgtccccaa cagcgccacg ccagaagatt gcgtcgcaga
    50341 ccagacgttg tcgccttcgg atgccagacc ttcgttgttg gcaaagttcg ccagtacaat
    50401 ctgccccagc agttgggttt gttcgttgga atagttgccg acaaccgtac cgtcatcatt
    50461 gatttgataa ctcaccagat cgcccggttt gtagccattc tgggtggttg ccacaatgtt
    50521 gttagcgccg gtattttgct gcatggagtt gaggaagctc aggctaaacg tggcgggttc
    50581 tgcgccgtta attgcgccgg tggtaatatt ctctgttgga tttgaggtca gaacgccatt
    50641 ggcattaaac accagcttca ttgcaggctc ggctgtacct gttggatcac tgctatcctg
    50701 ggtgtagacc tgccagttat tatccccggt cttcacaaag tagacgctca tgtcatgagc
    50761 attaccctga ctgtcgaaaa cagtcaccga accttttttg ttatagctat ccgcattgct
    50821 ggcatcaaat gcgttaacag agggaagcgg atcgctggaa ttcaggttga tctgcatcga
    50881 cgccgtggtg gtagttttcg ctgccatcag ggtattcggg atcgaaatgt tagtcggatt
    50941 cgccccttgc tgaatagtcg gcggcgtacc ggttgccggg taacccgtca gctgtaaacc
    51001 ttgcatattc accaggttac gattttcatc cagcttaaat tgtccgttac ggctgtagaa
    51061 caccgaaccg ttgctatcta ccagacggaa aaaaccgttc tggctgatag caacgtccag
    51121 accacgcccg gtgttggtgg tcgtgccatc ggtaaagtcc tgagtgatac cggcaacttt
    51181 tacccccagt cccactttcg aaccggcaaa catatcggca aaagaggccg tgcctgattt
    51241 aaagccgtag gtggcggagt tggcgatatt gttgccaata acatcgaggt tggtggcggc
    51301 agcgtttaat ccgctaaccg cttgagaaaa ggccatgact gactcctgaa atgtgaaggc
    51361 ttaaattatc tgccgtactt cgtcgagggt ggtggtgccg taagtgccga gatccagcgt
    51421 attaccgttg ttgccgcgga tcacaccctg caccagagca aactgcagcg gctgggcaac
    51481 cagttgtgta ccaccgttac tggcgctaat cgctacgttg taagaaccgt tcggcgcagt
    51541 gctgccgtca gtcaacgtac cgtcccaggt gaaactgtga actccggcgg tcagttcacc
    51601 aatatcaatg gtgcgcacaa ccgcgccatt tttatcggtg atggtggccg taactttgtc
    51661 tgccgcctgt tgcagctcaa caccaaacgg cgtggtcgtg gtcacagccc cttcttcact
    51721 gccggttccc gcaagaacag tggtgccggg gatcatcacg ccgtgaccga tcaggttact
    51781 ggcctgtaac gactggctgt tatcaatctg tccggaaata gatccgagcg tggtattgag
    51841 tttttcaatc ccgctgaccg tgctgatttg tgccaattgc gacgtcagct cgttgttttc
    51901 cattggattg gtcgggtcct ggtttttcag ctgcgccacc aacaaagtca gaaaactgct
    51961 ttgtaaatct gcggcgttgc tgcccgtgag cgaactactg ctggtggtac tgacgccggt
    52021 atttgtcgga tcggtggtgg ttaccgcaat ggacatagct ttctccttta ttgaccgagc
    52081 gtaagggttt tcagcatcat gcttttcacc gtgttgagca cttcaacatt ggcctgatag
    52141 ctgcgtgacg ccgacatggt gttaaccatc tctccgacaa catcaacgtt cggcattttt
    52201 acgtagccct ttgcatccgc cagcggatta cccggttcat aaaccagttt gtccggggcc
    52261 tgactttcta taacatcggc aacctttacg ccgcctgtcg cagcacctgg tgcagcgtta
    52321 acctggaata ccacctgttt tgcccgatat ggctgtccat caggaccggt cacgctatca
    52381 gcattcgcca gattactggc cgccacgttc aggcgctggg actgggcagt taacgccgac
    52441 ccggcgatat caaaaatatt cagcagtgcc atccgttaat ttccgctctg taaaacgttc
    52501 atcatgcctt tgatttgccc gctcaacgcg ctaaggctca tctggtattg caggctgtta
    52561 tcggcaaact gggtgcgttc gcgatccata tcgacggtat taccgtcaag cgaaggctgg
    52621 tccggaatac ggtattgcag ttctgcggta ggaggcgtca gcgcctgcgc cggaatgtgt
    52681 tgcgttgagg tcatcgtcag agcaaccaca ctggttgcat tccgtccacg ttgcatgact
    52741 tttttaagtt cactggcaaa atcgatatcg cgcgcctgat aaccaggggt atcggcattg
    52801 gcgatgtttg ctgccagcac ttcctgacgc tgggcgcgca gattgagcgc ctcttgttga
    52861 aaacgtaagg cggcgtcgag cttatcgagc atatctcctc cgcaggtatc aaaattctgc
    52921 catcagctta aatgccttta cgcgcgcgtt atcggcggaa taaacgcaaa atgggtcgct
    52981 atttatgccg ttgatggtca ttgtggacag gtacaattca cgttgtagaa atggctgggg
    53041 gcgaaaatgc tggcaataaa acgtagcgtg gcgatcatcg cgatactgtt cagtccgtta
    53101 agtgcggcga gcaatctcac atcgcaattg caaacctttt ttagctccca gctcgcaggg
    53161 ataagtgatg aggttcgtgt ttctattcgt acagcgccca atctactacc gccgtgcgag
    53221 cagccattgc tttcgatgag caataattcc cgcttgtggg gcaatgtgaa tgtgttggca
    53281 cgctgcggta acgacaaacg atatttacag gttaatgtgc aggccacagg aaattatgtg
    53341 gttgccgcga tgcccattgt gcgaggagga aagctgggag ctggcaatgt caaactgaaa
    53401 cgcggacggc tggatacctt gccaccgcgt acggtgctgg atatcaatca acttgttgat
    53461 gccgttagcc tgcgcgatct atcacccgat caacctatcc agttaaccca gtttcgccag
    53521 gcatggcggg taaaagcggg acaacgcgtc aacgtgatcg ccagcggtga tgggtttagc
    53581 gccaacgcag aaggtcaggc gctaaacaat gcagccgtcg cacagaatgc gcgggtgcgc
    53641 atggtatctg gacaggtagt cagcggcgtt gttgatgcag atgggaatat tcttataaac
    53701 ctgtaagctg ttaaagatta cccgtccctt gccgataaat aagcaacaca tgataaaagc
    53761 gccctcaatg aggaataaac catgagtatt gatcgcactt cgcctctgaa gcccgtaagc
    53821 accattcaac cgcgcgaaac cactgacgcg ccggtaacga acacccgagc ggcaaaaaca
    53881 acagcctcca ccagcaccag tgtgacgtta agcgacgcgc aagcaaaact gatgcaaccc
    53941 ggcagcagtg atatcaatct tgaacgtgtc gaagcgttaa aactggcgat tcgtaacggt
    54001 gaactaaaaa tggacaccgg caaaattgcc gatgcgctga tcaacgaagc gcagcaagac
    54061 ttgcagagta attaatcgta tgacgcgtct tgcagagatc ctcgaccaga tgtccgctgt
    54121 gcttaacgat ctcaaaacgg taatggatca agagcagcaa catctctcta tggggcagat
    54181 caacggcagc cagttgcaat ggattacaga acaaaaaagc tcactgctgg caacgttgga
    54241 ttacctcgaa cagttacgca ggaaagaacc caatacagca aatagcgttg atattagtca
    54301 acgctggcag gaaattgctg tgaaaacgca gcaactacgc caaatgaatc aacataacgg
    54361 ctggttactg gaaggacaga ttgagcgcaa tcaacaggcg ctggaaatgt tgaaaccgca
    54421 tcaggaaccg acgctatatg gggcgaacgg tcagacctca acaacccatc gcggcagtaa
    54481 aaagatttcg atctgaagat tatctccggc ctgcactgca ggccggaatg cattgttaca
    54541 ccgtccggcg ggcaaattct ttaactttga agcccagtac cgccagtgca gcgaagtacg
    54601 cggcaatccc cgccagcacg accgccatta aacgcagcaa acgccagggc atggtaccca
    54661 atgaccactc cggcatgata tgtaacatac ctaaaagcac gccagacatc accagtaccg
    54721 ccaccaccag acgcaacaga aacgccatcc agccaggttg cggggtaaag attttctgct
    54781 tacgcaactg ccagtaaagc agcgaagcat tcagacacgc cgccagacca atagaaagtg
    54841 acagcccggc atgtttcaac ggaccgataa acgccaggtt catcaattgc gttaaaatca
    54901 gcgtaacgat ggcaattttc actggcgttt taatgtcctg gcgggaataa aagccaggag
    54961 ccaacacttt cactacaatc aggccaatca aacccaccga gtaggcaatt aacgcccgct
    55021 gggtcatcag cgcatcaaag gcggtaaatt taccgtactg gaacagcgaa acggttaacg
    55081 gaccggaaag aatgcccaac gcaaccgcac tcggcaacgc caacaggaaa caaagacgca
    55141 gtccccagtc catcaaacgg ttgtattcat catgattgcc actggcaaaa cttttcgaca
    55201 gcgacggtag caaaatggtg ccaagcgcca cgcccagcac accggacgga aactccatta
    55261 agcggtcggc gtaatacatc catgacaccg aaccggaagc aagaaacgag gcaaaaatgg
    55321 tgttgatgat taaggagatc tggctcacag agacgccaag gattgccggt cccatctgtt
    55381 tcaccacacg cattgctccg gcatcgtgga agttaatgcg tggcaaaacc agcatgccga
    55441 tcttcttcag atgcggtagc tgatacacca gctgcaggac gccgccgacc gtcacagccc
    55501 acgccagcgc cagcaccggt gggttaaagt acggtgcggc aaacagcgcg aaaccaatca
    55561 tgctgatgtt aagcagtgtt ggagcaaacg ccggaatcga gaagcggttc cacgtattca
    55621 gaatcgctcc caccagcgac gccagggaga tcagcaagat atagggaaag gtaatcttta
    55681 gtagctggct ggtcagggca aatttgtcag ctgtgtcagc gaagcctggc gcggtcacca
    55741 tgatcaccca cggtgcggcg agcatgccag cgaccgtcac aaccgccagc gcaagtgtca
    55801 gcaggccaga aacataagag acaaagaccc gcgtggcgtc ttcaccctgc ttacttttat
    55861 attccgccag aatcggtaca aatgcctggg aaaatgcccc ttcggcaaag atacggcgta
    55921 acaagttagg aagtttaaaa gcgacgaaaa aggcgtcggt tgccatccct gcgccaaaga
    55981 ttctggcgac aattgcgtct cgtgcgaagc caagcacacg cgaaaacatg gtcatcgagc
    56041 tgacggcggc cagcgatttt aataaattca tcggtgttct aatccagacc cacgggcaaa
    56101 acgcctgcaa ggcaggcgct gaaagaggcg aatagtctac ctggattatg gtgaattgct
    56161 accgccagat gttacagggt ttattcactc atcgcatcgc gccagatctt gtcaacgata
    56221 cgttgcgcca gcactgcctg ttcgccggcg gtttgcggaa ctgtctgatt ttgcacgcat
    56281 tcaatgaagt gccgcgcaca gccgacaaat ccacgttgct caagcgtgct ctgccaacca
    56341 ggaatcggtt tattcactac accctgccca cgctcctcac gccattcgcg catttccgta
    56401 atgtcgatga gcgcaccgtc agtcacggcc tgcacggttt cacgctgact tccggcacgg
    56461 cgatgcatac aagtggtgat ctgcaaagga ccggccgaaa aatggtgctc ggcaaacagc
    56521 atttcgccag cgtcgttagt cagtagcgta ccgccatcca gagaggcttt gccgcccgac
    56581 aaccacagcg cggtatccac cacatgcaga taatcatcca gcaacgtgaa ataaagatcg
    56641 tgtggcccga cgctattgct acgatgttta tccattctta gcgaggctgc ggtggcgagt
    56701 tgcgttttta actcacagta gagtggtgcg aaacgacggt taaaaccgac catcaacgtc
    56761 agttttttcc gcgccgccag ttccaccagc cgttcagcat cgcgcagatt ttctgccagc
    56821 ggtttatcga cacagacatg tacccccgca ttgagtaacg tactgaccac gtcaaagtga
    56881 ctggcggtgc tggaatgtac aaaaaccgca tcgcaactgg cggcaaggct ggataacgaa
    56941 tcggcataag gaatgcgcca gctttcacaa attggcaggg ctttcgcgcg cgtaggcgac
    57001 caggctcctt gtaacgtcca gtcagacgct gccgccagca ccggtaacca cgctttttgc
    57061 gcaatgccgc ctaatcccac tacgccgata cgtaattttt tcactttaat ctcccaggtg
    57121 ggccagcaac gaatcaagac gctgtttcag ttctgccact tcgatttcca gggcttcgac
    57181 gcgggcctgt aaatcaccgt caaccgcgtt cgacatatcc gttaccgccg gctgatcctc
    57241 tacctcacca ctgaaaagat gcatgtagcg gctttcgcgt ttacccggtt cgcgggccag
    57301 acgcaccaca aaaggaccat cttcgcgatt cgccagttgt tccagcgtca actccacttc
    57361 cgccatatcg ctgaattcat acattcgcgc ggcgcggctg cgcagttcac ccggcgtctg
    57421 ggcaccacgc aataacaacg tggtgattaa cgccacttct gcggcgctca gtttcagatc
    57481 gccaaattct gaattacaaa aacgttgctc atatttggtg acccgattac caaaaccgct
    57541 cactgtgcgt agataatgac gtttgaccag attatccagc tgttcctgca cttcagattc
    57601 actcagattc atgaccggtt cacggttcgt tttctgatta caggccgtga ctacaccatt
    57661 gactgagagc gggtattgct ccggcgtggt cacctgcttt tccagcaagc aaccaatcac
    57721 acgagcttcc agggcagtaa gttgatattt catgagattc tccttagcgg ccgggcgtcc
    57781 agtctggggt agttaatgcc gtcagtacgt gatcgcgcca ttgtccatca atcaacagat
    57841 agtctttcgc atagccttct ttttcaaaac ccagtcgcgc cagtaaatca ccgctgcgtt
    57901 tattgtgcgg catataatta gccataatgc gatgaatatg ttgggtgcgc tgcatataac
    57961 gaatggctgc ggtcagggct tcaaacatga gtcctttgcc ctgccatttt tgcccaatcg
    58021 aataaccgag atagcaggca tgaaaagagc cgcgaacaac attggaaaaa ttggcaacgc
    58081 caataatctc tttttcgtcc gggtcgaata agccaaagta gaaagctgaa ccttgtttat
    58141 gaaattcgtt aatcatcccc agcctggcct gccagcctga tggataacag tggctttcgt
    58201 cgcgcactgg ctcccagggc ttgaggaaat ggcgattctc tgcgtaataa tccgccagac
    58261 gccaggcatc acgatcatgc accagacgca cgaccagtcg gtctgtggtt aagcgcactt
    58321 ttggcacgtt actgcgatag ccaaacattc tatacctact ccttcccgta acgtctttaa
    58381 tacgctgtaa gtattactat acctgcgcaa ccagaccctg tgaaaagtcc aaaatgccat
    58441 tttctgcatt attcacatat ttgataaaaa ctctctatca aagcgacgtt ttgataaaaa
    58501 aatattgtcc ccgaagtgat gggaaaagtc ttagcggcaa tgcagaatag aaagtgctca
    58561 tcttttattt cccgggaggg gaaatgtccc gcgtgtcgca ggcgaggaac ctgggtaaat
    58621 atttcctgct catcgataat atgctggtcg tgctggggtt ctttgttgtc ttcccgctga
    58681 tctctatccg cttcgttgat caaatgggct gggccgccgt catggtcggt attgccctcg
    58741 gcctacgcca gtttattcag caaggtctgg gtattttcgg cggcgcaatt gccgaccgct
    58801 ttggtgccaa accgatgatt gttaccggta tgctgatgcg tgccgccgga ttcgccacaa
    58861 tgggtatcgc ccacgaaccg tggctattgt ggttttcatg cctgctctcg ggactcggtg
    58921 gcacgttgtt tgatccgccg cgttcggcgc tggtggtgaa actaatccgt ccacaacagc
    58981 gtggtcgttt tttctcgctg ttgatgatgc aggacagtgc cggtgcggtc attggcgcat
    59041 tgttggggag ctggctgttg caatacgact ttcgcctggt ctgcgccaca ggggccgttt
    59101 tgtttgtgct gtgtgcagcg ttcaatgcgt ggttgttacc ggcatggaaa ctctccaccg
    59161 tacgcacgcc cgttcgcgaa ggcatgaccc gcgtgatgcg cgacaagcgt tttgtcacct
    59221 atgtcctgac gctggcgggt tactacatgc tggctgtaca agtgatgctg atgctgccaa
    59281 ttatggtcaa cgatgtggct ggcgcgccct ctgccgttaa atggatgtat gccattgaag
    59341 cgtgtctgtc gttaacgttg ctctacccta tcgcccgctg gagtgaaaag cattttcgtc
    59401 tggaacaccg gttgatggct gggctgttga taatgtcatt aagcatgatg ccggtgggca
    59461 tggtcagcgg cctgcaacaa cttttcaccc tgatttgtct gttttatatc gggtcgatca
    59521 ttgccgagcc tgcgcgtgaa accttaagtg cttcgctggc ggacgcaaga gctcgcggca
    59581 gctatatggg gtttagccgt ctgggtctgg cgattggcga cgctattggt tatatcggtg
    59641 gcggctggct gtttgacctg ggcaaatcgg tgcaccagcc agagcttccg tggatgatgc
    59701 tgggcattat tggcatcttc actttccttg cgctgggttg gcagtttagc cagaaacgcg
    59761 ccgcgcgtcg tttgcttgaa cgcgacgcct gatttgatac tcccccctga ctcctccata
    59821 ctgaaagtga actgacagcc aaatggagga gtcacgtgaa gctatacatt tacgatcact
    59881 gcccttactg cctcaaagcc cgcatgattt tcggcctgaa aaatatcccc gtcgaattac
    59941 atgttctgct caacgacgac gcagaaacac ccacccggat ggtcggtcaa aaacaggttc
    60001 ccattctgca aaaagatgac agccgctata tgccagaaag catggatatc gttcactatg
    60061 tcgataaact cgacggcaaa ccgttactga ccggcaaacg ttcccctgcc attgaagagt
    60121 ggctgcgcaa ggtcaatggc tacgccaaca aactgctgtt gccgcgtttt gccaaatcgg
    60181 catttgatga gttttctact cccgccgcgc gcaaatattt cgtcgacaag aaagaggcca
    60241 gcgcgggtaa ttttgccgac ctgctggccc actctgacgg tctgattaag aatatcagcg
    60301 atgatttacg tgcgctggac aaactgatcg tcaaaccgaa cgccgtgaat ggcgaacttt
    60361 cggaagatga tattcagcta ttcccgctac tgcgtaatct gacgctggta gccggaatta
    60421 actggccaag ccgcgttgct gattaccgcg ataatatggc gaaacagaca caaatcaatt
    60481 tgttatcatc aatggcgatt taatcctgac tcgccgcggc agcctgtatt cgcgggttgc
    60541 cgccgttttc agttttttct gctggcgagt ttatcgctgg tgacacatgc tgcgttgtgt
    60601 caggatattg acgcagattt catgtcgagg actaccatga acaagttttt atttgccgct
    60661 gcattgatcg tcagtggcct gctcgttggc tgtaatcaac tcacccaata caccatcacc
    60721 gaacaagaaa ttaaccagtc gcttgcgaaa cataataatt tctcaaaaga tattggttta
    60781 cccggtgtgg ctgacgccca tattgttctg acaaacctga ccagccaaat tggtcgcgaa
    60841 gagccgaata aggttgccct aaccggagac gccaatctgg acatgaactc cctgttcggt
    60901 agccagaaag cgaccatgaa actgaagctg aaagcgctgc ccgtgtttga taaagaaaaa
    60961 ggcgcgatct tcctgaaaga gatggaggtg gtcgatgcga cggtacaacc ggaaaaaatg
    61021 caaacggtga tgcaaacgtt gcttccctat ctgaaccagg cattacgcaa ttactttaac
    61081 cagcaacctg cttacgtcct gcgcgaagat ggcagccagg gcgaagcaat ggcgaaaaaa
    61141 ctggcgaaag gcattgaagt gaagccaggc gaaattgtca ttccatttac tgattaatca
    61201 cgagggcgca ttcgcgccct ttatttttcg tgcaaaggaa aacgtttccg cttatccttt
    61261 gtgtccggca aaaacatccc ttcagccgga gcatagagat taatgactgc accatcccag
    61321 gtattaaaga tccgccgccc agacgactgg caccttcacc tccgcgatgg cgacatgtta
    61381 aaaactgtcg tgccgtatac cagcgaaatt tatggacggg ctatcgtaat gcccaatctg
    61441 gctccgcccg tgaccaccgt tgaggctgcc gtggcgtatc gccaacgtat tcttcacgcc
    61501 gtacctgccg ggcacgattt caccccattg atgacctgtt atttaacaga ttcgctggat
    61561 cctaatgagc tggagcgcgg atttaacgaa ggcgtgttca ccgctgcaaa actttacccg
    61621 gcaaacgcaa ccactaactc cagccacggc gtgacgtcag ttgacgcaat catgccggta
    61681 ttagagcgca tggaaaaaat cggtatgccg ctactggtgc atggtgaagt gacacatgca
    61741 gatatcgaca tttttgatcg tgaagcgcgc tttatagaaa gcgtgatgga acctctgcgc
    61801 cagcgcctga ccgcgctgaa agtcgttttt gagcacatca ccaccaaaga cgcagccgac
    61861 tatgtccgtg acggaaatga acggctggct gctaccatca ctccgcaaca tctgatgttt
    61921 aaccgcaacc atatgctggt tggtggcgtg cgtccgcacc tgtattgtct acccatcctc
    61981 aaacgcaata ttcaccaaca ggcattgcgt gaactagtcg ccagcggttt taatcgagta
    62041 ttcctcggta cggattctgc gccacatgca cgtcatcgca aagagagcag ctgcggctgc
    62101 gcgggctgct tcaacgcccc aaccgcgctg ggaagttacg ctaccgtctt tgaagagatg
    62161 aatgctctgc agcactttga agcattctgt tctgtaaacg gcccgcagtt ctatgggtta
    62221 ccggtcaacg acacattcat cgaactggta cgtgaagagc atcaggttgc tgaaagcatc
    62281 gcactgactg atgacacgct ggtgccattc ctcgccgggg aaacggtacg ctggtctgtt
    62341 aaacaataaa aatcgtagct tcctgttgtc attaggttat tttacctgta taaataacca
    62401 gtatattcaa cagggggcta ttatgcgaat tgaagtcacc atagcgaaaa cttctccatt
    62461 gccagctggg gctattgacg ccctggctgg cgaactttcc cgccgtattc agtatgcgtt
    62521 tcctgataat gaaggccacg tatcagtacg ttatgccgca gcgaataatt tatcggttat
    62581 tggcgcgaca aaagaagata aacagcgcat tagcgaaatt ctccaggaaa cgtgggaaag
    62641 cgccgatgac tggtttgtca gcgaataata tgcagtgatt tttgtttttg ccgggtcgcc
    62701 ccggcttttt tattttactt gcgaattatg agtacaaaat ttctacactt cctcaaaata
    62761 attgcacaat atgctaaaaa aatatgaatg acatgctgtt ttattgttca tataaatctt
    62821 aaaagcagaa atttatctgt tccccccttt aaaaattcgg tgattaccct tatttattgg
    62881 tatactgaag ttgcttcaga taaaaacgca ttgaacctcg aataacgttg tctagtaaca
    62941 cgaattaggg ggccatgatg gaaaaaaata atgaagtcat tcagactcat ccgctcgtag
    63001 ggtgggacat cagcaccgtt gatagctatg atgcgctgat gttgcgtttg cactaccaga
    63061 ccccaaataa gtccgagcag gaagggactg aagttggtca gacgctctgg ttaaccactg
    63121 atgttgccag acaatttatt tcgatattag aagcaggaat cgccaaaatt gaatccggtg
    63181 atttcccggt aaacgagtat cggcgtcatt aacatgacat gaccatccac ccaaaaggca
    63241 cctcaccggt gcctttacca tttctgggtt gtatatctgc attcagttaa ttactctgct
    63301 taaaccatga gagcagagag aaaaaaatga aatacgatct catcattatt ggcagcggtt
    63361 ccgtaggcgc tgccgccggg tattatgcaa cccgcgccgg tttaaacgtg cttatgaccg
    63421 acgcccatat gccaccacat caacacggca gccaccacgg cgatacgcga ttaattcgac
    63481 atgcttatgg tgaaggcgaa aagtatgtcc cgctggtcct ccgcgcacaa acgctgtggg
    63541 atgacctctc ccgccacaac gaagatgatc ccatttttgt acgctctggt gtcattaacc
    63601 ttggcccggc tgactccgca tttctcgcca acgtcgccca cagcgccgaa cagtggcaac
    63661 tcaacgttga aaagctcgat gcgcaaggga ttatggcccg ctggccagaa atacgcgtcc
    63721 cggacaacta catcggctta tttgagactg attccggttt tttgcgcagc gaactggcga
    63781 ttaaaacctg gatccaactg gcgaaggaag cgggctgtgc gcaactgttc aactgcccgg
    63841 tcaccgcaat tcgtcatgac gatgatggcg taactattga aacggctgac ggtgagtatc
    63901 aggcgaaaaa agcgattgtc tgcgcgggaa catgggtaaa agacctgctc ccggagctgc
    63961 ctgtccagcc cgtacgtaaa gtatttgcct ggtatcaggc cgatggccgc tatagcgtga
    64021 agaataaatt cccggcgttt accggtgaac tgcccaatgg cgatcaatat tatggttttc
    64081 cggcagaaaa cgacgcgttg aagattggca aacataacgg aggccaggtt atccattcag
    64141 cggatgaacg tgttccgttt gcggaagtgg tcagcgatgg ttcggaagcc ttcccgttct
    64201 tgcgcaatgt attgccgggt atcggttgct gcctgtacgg cgctgcctgc acctatgata
    64261 attcgcctga cgaagatttt attatcgata ccctacccgg ccacgataat acactgctca
    64321 ttaccggcct gagtgggcac ggttttaaat ttgcgtcagt tttaggggaa atagctgccg
    64381 attttgcgca agacaaaaaa agcgattttg atttgacgcc attcagcctt tcccgcttcc
    64441 aataatcatc attacggcct ccagaatgag gccgttttta caggagtatt taatgcgtcg
    64501 gctgttgcac tatctcatca ataatattcg cgagcatctg atgctatatc tttttctctg
    64561 gggattgttg gcaattatgg atctgatcta tgtgttttat ttttagtttt ccgcactgac
    64621 ataaactgac aaatatttaa tctccctttc atttagcgtg aaaaattgaa tgagattatt
    64681 cctgcttcta cagcactcac cagacatctc tgatgctgaa cccactaaac ttatgaagat
    64741 tctttcatta ttaattcaaa attattgaaa taaaaataaa tatcattcaa tttgttattt
    64801 ttgtgttgca attcattctc cgacacgcca ttttctttct ctcacaaatt cgttttggat
    64861 gcacttatgt cattcacaaa tacccctgaa cgctatggtg ttatatctgc ggccttccat
    64921 tggctaagcg caattattgt ctacggcatg tttgctttag gtctgtggat ggtcacgctc
    64981 agttattacg atggctggta tcacaaagca cccgaactac ataaaagcat cggtattttg
    65041 ttaatgatgg ggctggttat tcgcgttctg tggcgtgtca tatctccacc gccgggtccg
    65101 ctgccgagtt attcgcccat gactcgtctt gccgcaaaag ctggacatct cgcattgtac
    65161 ctattgctgt tcgccatcgg catcagcggc tatctgatct caactgccga tggtaaaccg
    65221 atcagcgttt ttggctggtt tgacgtcccc gcgacccttg ccgacgctgg cgcacaggca
    65281 gactttgccg gtgccctgca tttttggctc gcgtggagcg tcgtagtact gtccgttatg
    65341 cacggattta tggccctgaa gcatcatttc atcgataaag acgacactct gaagcgcatg
    65401 ctgggaaagt cgtcatctga ctatggagta taaaaaatga aaaaaagcct gcttggttta
    65461 accttcgcgt ccctgatgtt ctctgccggt tcagcggttg ccgccgatta caaaattgac
    65521 aaagaaggtc agcacgcctt tgttaacttt cgcatccagc atcttggcta tagctggtta
    65581 tatggcacct ttaaagattt cgacggtact tttacctttg atgaaaaaaa tccagctgcc
    65641 gataaagtga atgtgacaat taacaccacc agcgtcgata ctaatcacgc cgaacgtgat
    65701 aaacatcttc gcagtgcaga tttcctcaat accgcaaaat atccacaggc aacattcacc
    65761 tccaccagcg tgaagaaaga cggtgacgaa ctggatatta ccggcgatct gacgctgaat
    65821 ggtgtaacca aacccgtcac gctggaagcg aaattaattg gtcagggcga cgacccatgg
    65881 ggtggtaaac gtgcaggctt cgaggccgaa ggcaaaatta agctcaagga cttcaatatc
    65941 aagacagatt taggtccagc ttctcaggaa gtggatctga ttatttcagt ggaaggcgta
    66001 cagcagaagt aagagtgaaa tgttgccgga tggcacgcat ccggcaatga tatttattct
    66061 gttggatcag gaatgcccag tgttgtattc agacgtccac gagacttatt aaagatctta
    66121 ttgccatttt cacgtcctgc ccgacggcgt cgctgttctt ctggcggtaa cgcgctttct
    66181 tcgcagcaaa tttcactaca acaacctttg tatttttccg cacatactgg acactgaata
    66241 aacagcaggt ggcagccatc atttttacag ttggtatggc tgtcgcacgg cgcaccgcac
    66301 tggtggcaat gcgcgataat ctcatcagat atacgttcgc ccatccgctc gtcaaaaaca
    66361 aaatttttgc caataaaacg caccggcaag ccctgctcgc gcgccttacg ggcgtattca
    66421 ataattccac cctcgatatg ccagacttta ttgaatccgt tatgtttcat ccaggcactg
    66481 gccttttcac aacgaatgcc gccggtgcag tacatgacga tttttttatc tttatgtgcc
    66541 tgcatcatct cgactgcttt tggcagctgc tcacggaagg tatctgccgg aatttccagc
    66601 gcgttttcaa agtgccccac ttcatactca tagtggttac gcatgtcgat aaacagtgca
    66661 tcgggatcgt caagcatggc gttcacttcc gccgcttgca gatactcacc aacattgctg
    66721 gcatcaaagt gaggatcgtc aataccgtcg gcaacgatac gatcgcggac cttcatgcgc
    66781 agtacccaga aggatttccc gtcatcatcc aacgcgatat tcaggcgtaa gccatctaaa
    66841 gccgagtcga aggcatagag ctgcgcgcga aatgttttaa cattgctcgc aggtacgctg
    66901 atttgcgcgt taatgccctc atgcgccaga tacactcgcc caaaaacatt cagcgcggta
    66961 aacagctgat ataaagcgtc acgggtcgcc ttaggatcgg cgatgtggaa atacttgtaa
    67021 aacgaaatgg tggttcgcgg ttcgctctca gccaacattt tggcttttaa cgcgtcgttg
    67081 gaaatgcggt tgtgtaacac tggcatggtg tacggttcct gcgagatggg aaagtaaaaa
    67141 tccgcggcat gatatagcaa ttatcgataa ttaacatcca cacattttac gctacatttg
    67201 cgcattaaaa attatttgtt atttacaagc gcggcaattt cgcccagtct tcagccacaa
    67261 attttggttg cgggcgaaaa aatgcgacaa tacatacaat tgcccgaata ggttgaaaaa
    67321 caggattgat atgacgaatc tacccaagtt ctccaccgca ctgcttcatc cgcgttattg
    67381 gttaacctgg ttgggtattg gcgtactttg gttagtcgtg caattgccct acccggttat
    67441 ctaccgcctc ggttgtggat taggaaaact ggcgttacgt tttatgaaac gacgcgcaaa
    67501 aattgtgcat cgcaacctgg aactgtgctt cccggaaatg agcgaacaag aacgccgtaa
    67561 aatggtggtg aagaatttcg aatccgttgg catgggactg atggaaactg gcatggcgtg
    67621 gttctggccg gaccgccgaa tcgctcgctg gacggaagtg atcggcatgg aacacattcg
    67681 tgacgtgcag gcgcaaaaac gcggcatcct gttagttggc atccattttc tgacactgga
    67741 gctgggtgcg cggcagtttg gtatgcagga accggggatt ggcgtttacc gcccgaacga
    67801 taatccactg attgactggc tacaaacctg gggccgtttg cgctcaaata aatcgatgct
    67861 cgaccgcaaa gatttaaaag gcatgattaa agccctgaaa aaaggcgaag tggtctggta
    67921 cgcaccggat catgattacg gcccgcgctc aagcgttttc gtccctttat ttgccgttga
    67981 gcaggctgcg accacgaccg gaacctggat gctggcacgg atgtccggcg catgtctggt
    68041 gcccttcgtt ccacgccgta agccagatgg caaagggtat caattgatta tgctgccgcc
    68101 agagtgttct ccgccgctgg atgatgccga aactaccgcc gcgtggatga acaaagtggt
    68161 cgaaaaatgc atcatgatgg caccagagca gtatatgtgg ttacaccgtc gctttaaaac
    68221 acgcccggaa ggcgttcctt cacgctatta aatctcccat gccggatgct tcagaatggc
    68281 atccggcatt accacagcaa atccccctga tttagcgata aaagctctct ggattgcgcc
    68341 ccctggaagt cgggcgcata attagtgtgc ttatcttttc ttcttatgtt caccgcgcct
    68401 ggcgcaccaa cagcggattg ctatgtcacc ctgtgaaaat gacaccccta taaactggaa
    68461 acgaaacctg atcgtcgcct ggctaggctg ttttcttacc ggtgccgcct tcagtctggt
    68521 aatgcccttc taacccctct acgttgagca gcttggcgtt accggccact ccgccctgaa
    68581 tatgtggtcc ggtattgtct tcagcattac atttttattt tcggccatcg cctcaccgtt
    68641 ttggggtgga ctcgccgacc gtaaaggccg aaaactcatg ctattacgct ctgctctcgg
    68701 catgggcatc gtgatggtgt tgatggggct ggcacaaaat atctggcagt ttttgatcct
    68761 gcgggcgctt cttgggttac ttggcggatt tgtccccaac gctaatgctc ttatcgccac
    68821 acaagtaccg cgtaataaaa gcggctgggc actgggtacg ctctccacag gcggcgttag
    68881 cggtgcgttg ctcggcccaa tggctggcgg cctgctcgcc gatagctacg gcttacgtcc
    68941 ggtattcttt attaccgcca gtgtgctcat actctgcttt ttcgtcaccc tgttttgcat
    69001 cagagaaaaa ttccagccgg tcagcaaaaa agagatgctg cacatgcggg aagtggtgac
    69061 atcacttaaa aacccgaaac tggtactcag cctgtttgtc accacgttaa tcatccaggt
    69121 ggcgacgggc tcaattgccc ccattctgac gctgtatgtc cgcgaactgg cgggtaacgt
    69181 cagtaacgtc gcctttatca gtggcatgat cgcctcggtg ccaggcgtag ctgctctgct
    69241 gagtgcacca cgactcggca aacttggcga tcgaatcgga cccgaaaaga tcctgattac
    69301 ggcgctgatc ttttctgtac tgctgttgat cccaatgtct tacgttcaga cgccattgca
    69361 acttgggatt ttacgttttt tgctcggtgc cgccgatggt gcactactcc ccgccgtaca
    69421 gacactgttg gtttacaact cgagcaacca aatcgccgga cgtatcttca gctataacca
    69481 atcgtttcgt gatattggca acgttaccgg accattgatg ggagcagcga tttcagcgaa
    69541 ctacggtttc agagcggtat ttctcgtcac cgctggcgta gtgttattca acgcagtcta
    69601 ttcatggaac agtctacgtc gtcgtcgaat accccaggta tcgaactgat ttttcgcctt
    69661 tcatacttgc aaaagcggag aatcagctat ccttttccct gaaacctcat caactcaaag
    69721 ggagaatcgt gatgaccatg tacgcaacgc ttgaagaagc cattgacgct gcacgcgaag
    69781 aatttcttgc agacaacccc ggcatcgacg ccgaagatgc gaatgtgcaa cagttcaatg
    69841 cccaaaaata cgttttgcag gacggcgaca tcatgtggca agttgagttt tttgccgacg
    69901 aaggggaaga aggtgaatgt ttacctatgc ttagcggtga agccgcgcaa agtgtttttg
    69961 atggcgacta tgatgagata gagatccgcc aggagtggca ggaagagaat acattacatg
    70021 aatgggacga gggggaattt cagcttgagc caccgctgga taccgaggaa ggacgcgcag
    70081 cagctgatga gtgggatgaa cgttaattac tcatacgggc catgatgaat gtcgatcggc
    70141 agcagtaaag tatcaaaaac cagggagaat ggcagatcaa ggattgtgac ataacgccag
    70201 gcggagtcac gcacatccca ttgcacaccg ggataatatt ggttgccatg cccctgcccc
    70261 ggtatagtgc ggctaatgat gctgccacag ccactcagca aggtcaccat gatgctcacc
    70321 acaattaaac gcatttgata cctcttccgt aaaaaaatac cggcataacg ccggtatttt
    70381 tattggctcg ttgtttttgc cagatgtggc gtgaacgcct tatccgacct acaaaatctt
    70441 gcaaaatcaa cacattgcag gagcgatgta ggcctgataa gcatagcgca tcaggcaact
    70501 acgtttttat tgcgaagccg catccggttt acgcaatgcc agtggattga gctttatccc
    70561 ttcgtaataa ctcacccatg aagaatatct ctcaggggaa ttccagacgc ggaaatgcag
    70621 acgggccatc gtcaccggat cgcttagcag cacaaggcga cgatcgcgat tcagcttctc
    70681 tggcgtctcg ttcagcgcct gttcaacatg gcggtcacgg gcgatttcca gcaccttact
    70741 ggcgcggtga cgcgcggtcg ccattgcggt tgccagagcg ttaaatgacg ggttaaacac
    70801 tgcgtgcata aaaccatcat caagggagcg ttgacggttc atctcaagga accgatcggt
    70861 atcaaccagt acctgcggcg gtgaatactc ttccgggatc aggaacagtt tccagcgttt
    70921 ggtgcgcaga ccaacggtgg cacggctgga aatcaccgaa acaaacggtg acaggatcaa
    70981 cgagaagaca atcggtgcca gccagaacag gaaacgcaga tccaaccacg ccatcccgac
    71041 agcccacact aaccctaaca gcagctgtga gccgtggcgt ttgaacgctt caccccagga
    71101 agtggagtca tcatcacgct gcggtgaatt ccacaccact tcccagccaa ggaacgcgct
    71161 gacgacgaag accgtatgga acagcatgcg taccggagcc agcagcacgg aaaacagcac
    71221 ttccagcagc aacgataatg taacgcgcca gaagccgcca tattctttcg ttcctttaca
    71281 ccaaataagc aaaatgctca atagctttgg cagaaacaac agcaccatgg tcgaagcaaa
    71341 aagtgcaatc gccagctcag gacgccactg cggccacacc gggaacaact gccgtggttg
    71401 caggaagtat tgcggttcgg tcaacgcatg cactacctgc aatgcagtag agagcgcgag
    71461 gaacataaac cacagcggag cggagagata agacatcacg cccgtcagga ataccgcacg
    71521 gtgaaccggg tgcataccct tcaccaggaa cagacggaag ttcatcaggt taccgtggca
    71581 ccagcggcgg tcacgtttta gctcatcaag caagttaggc ggtaattctt cataagaacc
    71641 cgggagatcg taagcaatcc agactcccca acctgcacgg cgcatcaacg ccgcttccac
    71701 gaagtcatgt gacaggattg aaccggcaaa ggaaccttcg cccggcagcg gggccagtgc
    71761 acagtgctcg ataaacggtt tcacgcggat aatcgcgtta tgcccccagt agtgcgactc
    71821 gccaagttgc cagaagtgca aaccggctgt aaacagtggc ccatacacgc gggtcgcgaa
    71881 ctgctgacag cgtgcataca gcgtatccat accggacgct ttcggcgacg actgaatgat
    71941 cccggcgttc gggttggctt ccatcaggcg caccaggccg cacaaacaat caccggtcat
    72001 taccgagtca gcatccagca ccaccatata gctgtactgg ctgccccagc gacggcagaa
    72061 gtcatcgata ttaccgcttt tacgcttcac gcgacggcgg cggcggcgat agaaaatctg
    72121 accttcgcca ccgacttcag cgataagctc catccaggct ttttgctctg cgacgcagat
    72181 atccgggttg taactgtcac tcagaatgta gacatcaaag tgtttggcat tcccggtggc
    72241 ttttactgat tcccacgttg cacgcaagcc agcaaaaaca cggttcacgt cttcgttaca
    72301 gataggcatg atcaacgccg tgcgatgctc cgggtttaat ggttcatcgc caactgttga
    72361 cgcagatata ctgtatttat cgcgaccaat aagtagttgc aggaagccca ttaacgccgt
    72421 ccagaatccg gcggacaccc aacagaacag taccgcaaag aggatcagga taccggtttg
    72481 cagcatataa ggcagaagct gcataaagga aacccacaaa tcctgaccaa ccatatccat
    72541 aggattaatc agcgcccaac cctgataagg aagaatggtc ttcatatacc aggtcgcgac
    72601 gacagtttgc gcgagcgtca ggatcaacag aatgtaacgg cggatggtac cgacggtacg
    72661 ccacttttgc tcgctctcct gctcttcttt ggtcaaacga gccagatagc gcggtgttac
    72721 atcgcgtcca cgcaggcgat cccagaaacg gcctaccggg ttggtacgcc acgggtcggg
    72781 aaacatcgag gagcgttttg cttctggcat cgccttaagc tgatcgcgcc cttcgtcatc
    72841 tttaattaac tgtccatcag caagtgaatc tggccaggct tgttccagac gcgcctttac
    72901 cgagccttgc ggggagtcat cctcccgcgc ccaggtgcgg tgttcggcat ccagcgcctg
    72961 atgaacggcg cggatatcag tcttcggcaa tgccgctttc tcgcttgcgg cgatgggcat
    73021 tgcgtcaatg tactcagttg tcttattcat tggcaggtaa ctggtagctc caggtttcac
    73081 tcaacgtctg atcggcattc accagcgcag cgcgcatttc agtggttttc ttggcatctt
    73141 tcactttcac acgcatcacc agacgccagc ctttggtgac cgggttatag cgcaccgtgc
    73201 tttcaactat ctcaccatta tcaccaatgc tggtttgcgc tgtgaccggg gtatcctctg
    73261 gcagtttttt catctctgcg ccggtaaaat cgaccacaaa ggcgatagta ccgtcaggct
    73321 ggcgaatcag gttcgactgc ttcacatccc ccgttgaacg acgcgtttgt tgcacccatg
    73381 cgttatctgg cgcatgcagt ttgtcttcat cacggctgaa ggtgatggtg tatttaaagt
    73441 tcatctcttt acccggctcc ggcagctgat ccggcgtcca gtaagcgacg atgttatcgt
    73501 tagtttcatc gttggttgga atttccacca gctcaacgct gcctttaccc cactcccctt
    73561 tcggagtcac ccatgcgctt ggacggagat cgtaacgatc atcgagatct tcaaagcggg
    73621 agaaatcacg accgcgctgc aacagaccaa agccttgcgg gttttccatg gagaagctgc
    73681 tgaccgctaa atgtttcggg ttattcaacg gacgccagat ccattcgcca ttaccggcat
    73741 ggatagagag accgttagag tcgtgcaact ccggacgata gttatttgct ggcgacggtt
    73801 ggttcggccc aaacaggaac atactggtta acggtgcaac ccccagtttg ccgactttat
    73861 cgcgcagata gattttcgac tgcacatcca caaccgtgtc acgccctggc ataactacga
    73921 atttgtaagc acctgtcgcg cgcggcgagt caagcaatgc ataaatggtt aaacgtttat
    73981 cagttggttt tggacgctcg atccagaact ctttgaagcg tggaaattct tcacccgatg
    74041 gcaaggcggt atcaattgcc agaccgcggg cagaaaggcc ataaacctga cctgcaccaa
    74101 tcacgcggaa atagctggcc ccgagcatgc tgacgatttc atcgttttta tctttgctgt
    74161 tgatcgggta aagcacttta aaaccggcaa aaccaaggtc ttttaccgtg tctttgtcat
    74221 gctgaacatc gccgaaagtg aaataatccg ggctgtattt gattcgtttg actgcggtgg
    74281 cagtcacttc atttattttg accggggtat cgaagtacat accctgatgg tagaactcga
    74341 gtttgaatgg ggtcttcaga ttgttccagt acgctttgtc atgattaaac tggatctgct
    74401 gatagtccgc gtatttcata tcgcggaaaa cggagggcaa gttgcttttg ggcgcctcat
    74461 agcctttccc ggctaaggat tgagcttgct ttgcgacatc atcaatactg aaagcccagc
    74521 ttgaagatgt atacagggtt aacattactg cagcactcaa ccaacgcatt ttcatcattt
    74581 gtagtttatg tttcataatt agtaagcact tccccctttg tgtgcttata tcgatccgat
    74641 ctattttaat ggataaccag ccattccgac aacagaatcc cggtatcgtt cagcacgctg
    74701 gctcatggat taagcacctg attgcaaccg ttttcacttt ccgtgcttct cctaaagaca
    74761 gaagcatcga cttcgtgtag ggtttgccag caaatttatt tatcaaccgt cttacagacg
    74821 cttccattct ggttagtggt agcgatgatg ctatgcataa aaacttgaag cctgcgcctg
    74881 gcgcggtcag gtcggtgtcg ctatggcaac aagcctaaag gcataacccg acagggataa
    74941 gacgaaaaat ccgagattag ttaaccatat atgaatccag tacccgcgca acgtgaatat
    75001 ttcctcgact ccatccgcgc ctggctgatg ttgttaggga tcccttttca tatttcttta
    75061 atctattcga gccatacatg gcatgtgaat agcgccgaac cgtcattgtg gctgaccctt
    75121 tttaatgact tcatccactc gttccgcatg caggtatttt tcgttatatc cgggtacttt
    75181 tcctacatgc tttttttacg ctatcccttg aaaaaatggt ggaaagtacg tgtcgaacgt
    75241 gtagggatcc cgatgttaac agccatcccc ctactgacat tgccgcaatt tattatgctg
    75301 caatacgtca aagggaaagc ggaaagttgg cctgggctgt cattgtatga caaatataat
    75361 acgttggcct gggaattaat atcacatctg tggtttttac tggtgttagt ggtcatgacg
    75421 acgctgtgcg tatggatatt taaacgcatc agaaataatt tagaaaattc tgataaaacg
    75481 aataaaaaat tctcgatggt aaaactatcg gtgatttttt tgtgcctcgg catcggttat
    75541 gcggtaataa gaagaacgat ttttattgtg tatccaccca ttctgagtaa tggcacgttc
    75601 aattttattg tcatgcaaac gctattttat ttaccgttct ttatcctcgg cgcactggct
    75661 ttcattttcc ctcatcttaa agccttgttt accacgccgt ctcgtggctg taccctcgct
    75721 gcagcattgg cgtttgtcgc ttatttactc aaccagcgct atggcagtgg cgatgcctgg
    75781 atgtacgaaa ccgagtcggt gatcaccatg gtcctcggtc tgtggatggt gaatgtggtc
    75841 ttctcctttg gccaccgttt gcttaacttc cagtcagcgc gggtgactta ttttgttaac
    75901 gcatcgctgt ttatctatct ggttcaccac ccgttaacgc tgtttttcgg cgcatacatt
    75961 acaccgcaca tcacctccaa ctggcttggt tttctctgtg gcctgatatt cgtagtaggg
    76021 attgcgataa ttctgtatga aattcatttg cgcatcccgt tactgaagtt tttgttctct
    76081 ggtaaaccgg ttgttaagcg tgagaacgat aaagcaccag cccgttaagc cacatttaca
    76141 ataaccattc cacgggcaat atcgacgcca gtctgaccat aacccgcttc cagaaactgg
    76201 tagcgggttc ttttttgaga acaatctctt tctttgcatg acgatcaacc cagttgatcc
    76261 gtccccacct gtccagacgg agctgccagg ccgcatcata ctggctctga ataaagcgtt
    76321 tatcaattaa ctgagccagc gtttcgctct ctatcacgaa gcccatttca gtattgagca
    76381 atgttgaacg cggatcgaaa ttgaaagaac cgataaacac cgttttacca tcgatgctaa
    76441 aggttttagc atgcaggctg gcaccggaat taccggttat gccgcgatcg tgtaacgtac
    76501 tactttgttc acgcgtcggc ttgagttcat ataattccac gccatagcgg agcaattttt
    76561 tgcgccagcg cgcgtatccg gcatggacga cagcaacatc gttagcggca agagaattgg
    76621 ttaggatcgc aatctttacc ccttttctca ccatccgtaa gagttgcgcc acacctgcgc
    76681 gtgtcggtac aaaataggaa gagataatat cgatgcgttc actgggtgag cccatgatat
    76741 cgaacaggcg ctgcggtaac agtgaatgcc gttttgcctt gccctcccct ttcgccggat
    76801 catcacttaa taaacgtgtt ttcgcccaga taagcggcaa cgttccatca accagatgat
    76861 ttataaacgg actggattcc attttgcgta agtaacgatg cgtcatggca tcgttatgcc
    76921 aggaggcggg taactcgatg cgatccgcca tttcaccttc cgggacatcc agcacctgct
    76981 gtaagggtga aaccgatttg caataccagt agcgggcgaa atcatcggca acgtcctcta
    77041 ctacgggtcc tattgccatg acatctaaat ccgaaaaaag tggctcttcc cctgccccaa
    77101 aataggcatc accaatattt cgccccccta ccaacgtcac cacgccatcg acagtgaaac
    77161 ttttattgtg catacggcga ttgagacggg aaaagtcggt gatataacca agcggacgca
    77221 gcaagcgaaa cgagaaagga ttaaaaagcc ggacttcaat gcggggatgg ctgtcaagca
    77281 agcgtaaaat gtcgtcaagt ccgggcgtat tgttgtcgtc cagcaacaaa cggacgcgaa
    77341 cgccacgctt tgctgcggct aacagggcgg aaaacagtaa ccgacccgac atatcgtcct
    77401 gccagatgta atactgaaca tccagcgtat gctctgacat ttcggccaga cgataccggg
    77461 cggcaaacgc atccagactt ttctccagag ggaaaagacc acactgaccg ggatgttgcg
    77521 aacacagtgg cagcaccgcg ctcgccagcc ggggcaaatc attcatctcc ttgttgggta
    77581 aggagtcttt cgtagaggtg ggcgttttct tcatcataac agacaaagta tacctgttcg
    77641 ggtaaagcgt gacgggtaat aaattctgaa acggttttta ctgcgatttc agccgctgcc
    77701 gcacgagggt aaccataaac cccagtactg attgcaggaa aagccactga cgtatagctg
    77761 tttgccgcca ccagtcgtag gctattgaga taggcatcct gcaaaagctg gtcttcgttt
    77821 tgttcaccac cacgccagac tggcccgacg gtgtgcacta cggctttagc aggaagatcg
    77881 cctgcaagcg taataacggc atgccccgta gggcaatcgc cctgctgttg cctgactttt
    77941 aaacaagcat ccagcagggc cggacccgct gcgcgatgaa tggccccatc gacgccgcca
    78001 cctcccatta atgaaggatt agccgcattc acaatcacat caacggccag tttggtaata
    78061 tcaccctgca caacatgaat acgcgttttc ataacgcctc cttacacctt cttgtttctt
    78121 aagtgtacag caggcttttt ggttaaatcg catttttctg ctcagcaacg tgagttgttc
    78181 agaaacttag ttatattcga tgttcattac agccagcttt ttctgtttat caagatactg
    78241 cacttttact gaggctattt gcgcgttttt gacattgccg ctcattaacg ctttgctgga
    78301 gtaggtttta cccggaacag aaccattctg tgggcagttc atttctacgg gtgatgactg
    78361 ggtgctgacg tcacatgccg gttccacaat ttggccataa aaatggatca cgcccccttg
    78421 cgcggtgctt cctgcgatgg caataaaagg aaaaaacaaa ctgccgagca taagagagtt
    78481 taaaaaaggt ctgaacattt attctatttc ctcaatgatt agtcatcctt gagggttgtg
    78541 ttatccatac tttcaggttg gctgattcgc agcagaccat tctctccaga ttcatcttat
    78601 gctcgatatt tcaacaaatt aagacttttc tgaagagggc ggccattgtt gtgataaatg
    78661 aagtgactgt ccatcagaaa cagtaacaac tattttcacc cgatcgtccg gggaaatatt
    78721 taaactcaac ttcgtcaaag caatgggttg attagcaggc aatgaaaggg tcttttcttg
    78781 cttcgtctga ctttgccctg aactgccttc gcgcagggac aatatttgta ctctgcacag
    78841 acaagattga gtaagagtga cttcaggaat aatggtgtac atatcccctt gctgggtcgt
    78901 attaaaggtt atctgactgg aaagtgccgc aaggagtaat aacgcattca tattcttctc
    78961 ccgaaaaaaa acagggcttg cgccctgttt ctttaataca gatgatgtat tagtactgat
    79021 gagcggtcgc gttgttacca aagccaacct gagttacgtt gacggtggaa ttagatgcag
    79081 tctggtcaac cgctgcaccg ttgccgccac cgaattgttt aactgtcata tgagagtctt
    79141 taccgttcca ctgatcaaga gtggcgctgt taccaaagcc acgttgggtc agatcgattg
    79201 agctgtcatc tgagccctga ccaacatctg caccgttacc accaccatgc tgggtaatag
    79261 taagatcaga gttacgagca tcagcttgca gagcaagtgc agagttacca ccaccgtact
    79321 gataaatatt cagctctgaa ttcgggccgc tgttattacc gccaccaccg tggttaccgc
    79381 caccgccgcc gtactgagga acaacacctg ccagagcgct accggagaat acgattgctg
    79441 caattgctgc tacttttaaa agtttcatgt aaaaccccca tcggattgat ttaaaagtcg
    79501 aatggaaatt aacgttgtgt cacgcgaata gccatttgcg actgtctctg cactacaatt
    79561 gccgtttttt gagtaccata ctgtgtaata tttgctttat taccagaacc tttctggata
    79621 atcatcgcag tattaccata agcaccttgc gaaatactgg catcattggc actgcccgcc
    79681 tgatcaatat atgcaaggtt ataatctcct gtctggtcaa tctttgcccg gttgctacta
    79741 ccttcttgcg caacaaccgc caaaagtttt gagcctccct gccgtaactg agcactatta
    79801 ttagtcccag cttgaccaat tatggctgcc tgattaaatg aagacttact caattcattt
    79861 accgcgaagt tatattctga attagctaaa tcataacctg ctgcggctgc aatcccaggc
    79921 gcacccagta ttgttaacat cataaataac aatttgtttt tcatgttgtc accctggacc
    79981 tggtcgtaca tttaagaaat taaatcattt caacttggtt gttaacgcaa cctatatttt
    80041 gttaacgctg cgttacgatg gaaagtatgt ctgcggaaat atttttaata actcacccgc
    80101 gcgttgtatt ttt
//
LOCUS       DAZKIP010000009        80054 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZKIP010000009 DAZKIP010000000
VERSION     DAZKIP010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 80054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.009"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..209)
                     /locus_tag="ACHOUK_000711"
     CDS             complement(<1..209)
                     /locus_tag="ACHOUK_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFP9896221.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHOUK_000712"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHOUK_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFP9896222.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHOUK_000713"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHOUK_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFP9896223.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHOUK_000714"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHOUK_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFP9896224.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHOUK_000715"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHOUK_000715"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFP9896225.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSGQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHOUK_000716"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHOUK_000716"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFP9896226.1"
                     /translation="MKRIAFVFSTVPHGTAAGREGLDALLATSALTDELAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLDLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHOUK_000717"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHOUK_000717"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFP9896227.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANHLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDEMEAGRLGLASSN
                     LQQGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /gene="yheO"
                     /locus_tag="ACHOUK_000718"
     CDS             complement(4598..5320)
                     /gene="yheO"
                     /locus_tag="ACHOUK_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9896228.1"
                     /translation="MSRSLLTNETSELDLLDQRPFEQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHOUK_000719"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHOUK_000719"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFP9896229.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /gene="slyX"
                     /locus_tag="ACHOUK_000720"
     CDS             6520..6738
                     /gene="slyX"
                     /locus_tag="ACHOUK_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFP9896230.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHOUK_000721"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHOUK_000721"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP9896231.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /gene="yheV"
                     /locus_tag="ACHOUK_000722"
     CDS             complement(7472..7672)
                     /gene="yheV"
                     /locus_tag="ACHOUK_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFP9896232.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7709..9487)
                     /gene="kefB"
                     /locus_tag="ACHOUK_000723"
     CDS             complement(7709..9487)
                     /gene="kefB"
                     /locus_tag="ACHOUK_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFP9896233.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERR"
     gene            complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="ACHOUK_000724"
     CDS             complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="ACHOUK_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFP9896234.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10169..12082
                     /gene="yheS"
                     /locus_tag="ACHOUK_000725"
     CDS             10169..12082
                     /gene="yheS"
                     /locus_tag="ACHOUK_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9896235.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12082..13104
                     /gene="yheT"
                     /locus_tag="ACHOUK_000726"
     CDS             12082..13104
                     /gene="yheT"
                     /locus_tag="ACHOUK_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFP9896236.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQAQHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13098..13316
                     /gene="yheU"
                     /locus_tag="ACHOUK_000727"
     CDS             13098..13316
                     /gene="yheU"
                     /locus_tag="ACHOUK_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFP9896237.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13370..14239
                     /gene="prkB"
                     /locus_tag="ACHOUK_000728"
     CDS             13370..14239
                     /gene="prkB"
                     /locus_tag="ACHOUK_000728"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFP9896238.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAIELIML
                     PLVQRLMEGKKIE"
     gene            complement(14294..14698)
                     /gene="yhfA"
                     /locus_tag="ACHOUK_000729"
     CDS             complement(14294..14698)
                     /gene="yhfA"
                     /locus_tag="ACHOUK_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFP9896239.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15000..15632
                     /gene="crp"
                     /locus_tag="ACHOUK_000730"
     CDS             15000..15632
                     /gene="crp"
                     /locus_tag="ACHOUK_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFP9896240.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15683..17773
                     /gene="yhfK"
                     /locus_tag="ACHOUK_000731"
     CDS             15683..17773
                     /gene="yhfK"
                     /locus_tag="ACHOUK_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFP9896241.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17840..19060)
                     /gene="argD"
                     /locus_tag="ACHOUK_000732"
                     /gene_synonym="dapC"
     CDS             complement(17840..19060)
                     /gene="argD"
                     /locus_tag="ACHOUK_000732"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFP9896242.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPSDIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGQARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="ACHOUK_000733"
     CDS             complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="ACHOUK_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFP9896243.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKVVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPDSLPACFEVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19741..20343)
                     /gene="fic"
                     /locus_tag="ACHOUK_000734"
     CDS             complement(19741..20343)
                     /gene="fic"
                     /locus_tag="ACHOUK_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFP9896244.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKTKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20333..20500)
                     /gene="yhfG"
                     /locus_tag="ACHOUK_000735"
     CDS             complement(20333..20500)
                     /gene="yhfG"
                     /locus_tag="ACHOUK_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFP9896245.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRSHYER"
     gene            complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="ACHOUK_000736"
                     /gene_synonym="rotA"
     CDS             complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="ACHOUK_000736"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFP9896246.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21448..22629
                     /gene="tsgA"
                     /locus_tag="ACHOUK_000737"
     CDS             21448..22629
                     /gene="tsgA"
                     /locus_tag="ACHOUK_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFP9896247.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVAKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22891..25434
                     /gene="nirB"
                     /locus_tag="ACHOUK_000738"
     CDS             22891..25434
                     /gene="nirB"
                     /locus_tag="ACHOUK_000738"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFP9896248.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25431..25757
                     /gene="nirD"
                     /locus_tag="ACHOUK_000739"
     CDS             25431..25757
                     /gene="nirD"
                     /locus_tag="ACHOUK_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFP9896249.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25883..26689
                     /gene="nirC"
                     /locus_tag="ACHOUK_000740"
     CDS             25883..26689
                     /gene="nirC"
                     /locus_tag="ACHOUK_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFP9896250.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26708..28081
                     /gene="cysG"
                     /locus_tag="ACHOUK_000741"
     CDS             26708..28081
                     /gene="cysG"
                     /locus_tag="ACHOUK_000741"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFP9896251.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28336..28503
                     /gene="yhfL"
                     /locus_tag="ACHOUK_000742"
     CDS             28336..28503
                     /gene="yhfL"
                     /locus_tag="ACHOUK_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFP9896252.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28799..30136
                     /gene="frlA"
                     /locus_tag="ACHOUK_000743"
     CDS             28799..30136
                     /gene="frlA"
                     /locus_tag="ACHOUK_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFP9896253.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPATTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTPLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            30157..31179
                     /gene="frlB"
                     /locus_tag="ACHOUK_000744"
     CDS             30157..31179
                     /gene="frlB"
                     /locus_tag="ACHOUK_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFP9896254.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHTIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDARCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPHAEIGKIKNDLKQLPNALGYLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAAISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31230..32057
                     /gene="frlC"
                     /locus_tag="ACHOUK_000745"
     CDS             31230..32057
                     /gene="frlC"
                     /locus_tag="ACHOUK_000745"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HFP9896255.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDE"
     gene            32057..32842
                     /gene="frlD"
                     /locus_tag="ACHOUK_000746"
                     /pseudo
     CDS             32057..32842
                     /gene="frlD"
                     /locus_tag="ACHOUK_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
     gene            32942..33673
                     /gene="frlR"
                     /locus_tag="ACHOUK_000747"
                     /gene_synonym="yhfR"
     CDS             32942..33673
                     /gene="frlR"
                     /locus_tag="ACHOUK_000747"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFP9896256.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERISAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33804..34808)
                     /gene="trpS"
                     /locus_tag="ACHOUK_000748"
     CDS             complement(33804..34808)
                     /gene="trpS"
                     /locus_tag="ACHOUK_000748"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFP9896257.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGDIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(34801..35559)
                     /gene="gph"
                     /locus_tag="ACHOUK_000749"
     CDS             complement(34801..35559)
                     /gene="gph"
                     /locus_tag="ACHOUK_000749"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFP9896258.1"
                     /translation="MSKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(35552..36229)
                     /gene="rpe"
                     /locus_tag="ACHOUK_000750"
     CDS             complement(35552..36229)
                     /gene="rpe"
                     /locus_tag="ACHOUK_000750"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFP9896259.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(36247..37083)
                     /gene="dam"
                     /locus_tag="ACHOUK_000751"
     CDS             complement(36247..37083)
                     /gene="dam"
                     /locus_tag="ACHOUK_000751"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFP9896260.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(37190..38476)
                     /gene="damX"
                     /locus_tag="ACHOUK_000752"
     CDS             complement(37190..38476)
                     /gene="damX"
                     /locus_tag="ACHOUK_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFP9896261.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(38568..39656)
                     /gene="aroB"
                     /locus_tag="ACHOUK_000753"
     CDS             complement(38568..39656)
                     /gene="aroB"
                     /locus_tag="ACHOUK_000753"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFP9896262.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGTFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(39713..40234)
                     /gene="aroK"
                     /locus_tag="ACHOUK_000754"
     CDS             complement(39713..40234)
                     /gene="aroK"
                     /locus_tag="ACHOUK_000754"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFP9896263.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(40635..41873)
                     /gene="hofQ"
                     /locus_tag="ACHOUK_000755"
     CDS             complement(40635..41873)
                     /gene="hofQ"
                     /locus_tag="ACHOUK_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFP9896264.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(41785..42189)
                     /gene="hofP"
                     /locus_tag="ACHOUK_000756"
     CDS             complement(41785..42189)
                     /gene="hofP"
                     /locus_tag="ACHOUK_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFP9896265.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(42179..42619)
                     /gene="hofO"
                     /locus_tag="ACHOUK_000757"
     CDS             complement(42179..42619)
                     /gene="hofO"
                     /locus_tag="ACHOUK_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFP9896266.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQHWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVFWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(42603..43142)
                     /gene="hofN"
                     /locus_tag="ACHOUK_000758"
     CDS             complement(42603..43142)
                     /gene="hofN"
                     /locus_tag="ACHOUK_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFP9896267.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     SAEARVDAVLLQGEQQLARSLQITKPRLLERQQIREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(43126..43920)
                     /gene="hofM"
                     /locus_tag="ACHOUK_000759"
     CDS             complement(43126..43920)
                     /gene="hofM"
                     /locus_tag="ACHOUK_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFP9896268.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDARRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGWRLGRRTDEPAN"
     gene            44040..46592
                     /gene="mrcA"
                     /locus_tag="ACHOUK_000760"
     CDS             44040..46592
                     /gene="mrcA"
                     /locus_tag="ACHOUK_000760"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFP9896269.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(46757..47317)
                     /gene="nudE"
                     /locus_tag="ACHOUK_000761"
     CDS             complement(46757..47317)
                     /gene="nudE"
                     /locus_tag="ACHOUK_000761"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFP9896270.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVFEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            47637..49772
                     /gene="igaA"
                     /locus_tag="ACHOUK_000762"
     CDS             47637..49772
                     /gene="igaA"
                     /locus_tag="ACHOUK_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFP9896271.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHLVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            49837..50505
                     /gene="yrfG"
                     /locus_tag="ACHOUK_000763"
     CDS             49837..50505
                     /gene="yrfG"
                     /locus_tag="ACHOUK_000763"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFP9896272.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            50516..50917
                     /gene="hslR"
                     /locus_tag="ACHOUK_000764"
     CDS             50516..50917
                     /gene="hslR"
                     /locus_tag="ACHOUK_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFP9896273.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            50942..51820
                     /gene="hslO"
                     /locus_tag="ACHOUK_000765"
     CDS             50942..51820
                     /gene="hslO"
                     /locus_tag="ACHOUK_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFP9896274.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(51883..53607)
                     /gene="yhgE"
                     /locus_tag="ACHOUK_000766"
     CDS             complement(51883..53607)
                     /gene="yhgE"
                     /locus_tag="ACHOUK_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFP9896275.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLSGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQTDGMTPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWSELFKLVGITFFKTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFIAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRKGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEHSGRYGRAALESLKSDAEYMKDPKRARDLLMALDGEQHLQQQISEKV
                     LAENVLIAPGSGKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGRAERILFAF
                     DDERYIVYGFDPDKKEWQELTMSLLPRDITKEKLLTAAKDGKLGTKPKAWRDLVVDGE
                     RLDVNLNE"
     gene            53986..55608
                     /gene="pckA"
                     /locus_tag="ACHOUK_000767"
     CDS             53986..55608
                     /gene="pckA"
                     /locus_tag="ACHOUK_000767"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFP9896276.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTKQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVSEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(55684..57036)
                     /gene="envZ"
                     /locus_tag="ACHOUK_000768"
     CDS             complement(55684..57036)
                     /gene="envZ"
                     /locus_tag="ACHOUK_000768"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFP9896277.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQSTTKEG
                     "
     gene            complement(57033..57752)
                     /gene="ompR"
                     /locus_tag="ACHOUK_000769"
     CDS             complement(57033..57752)
                     /gene="ompR"
                     /locus_tag="ACHOUK_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFP9896278.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            57980..58456
                     /gene="greB"
                     /locus_tag="ACHOUK_000770"
     CDS             57980..58456
                     /gene="greB"
                     /locus_tag="ACHOUK_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFP9896279.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            58553..60874
                     /gene="yhgF"
                     /locus_tag="ACHOUK_000771"
     CDS             58553..60874
                     /gene="yhgF"
                     /locus_tag="ACHOUK_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFP9896280.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLGYLRELEERRQAILKSISEQGKLTDDLANAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAENEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNDRPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            61312..61539
                     /gene="feoA"
                     /locus_tag="ACHOUK_000772"
     CDS             61312..61539
                     /gene="feoA"
                     /locus_tag="ACHOUK_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFP9896281.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            61556..63877
                     /gene="feoB"
                     /locus_tag="ACHOUK_000773"
     CDS             61556..63877
                     /gene="feoB"
                     /locus_tag="ACHOUK_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFP9896282.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            63877..64113
                     /gene="feoC"
                     /locus_tag="ACHOUK_000774"
     CDS             63877..64113
                     /gene="feoC"
                     /locus_tag="ACHOUK_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFP9896283.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            64316..65194
                     /gene="rpnA"
                     /locus_tag="ACHOUK_000775"
     CDS             64316..65194
                     /gene="rpnA"
                     /locus_tag="ACHOUK_000775"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFP9896284.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDIT
                     VMPDDEIMQHRRMTLLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASQ"
     gene            complement(65221..65991)
                     /gene="bioH"
                     /locus_tag="ACHOUK_000776"
     CDS             complement(65221..65991)
                     /gene="bioH"
                     /locus_tag="ACHOUK_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312282.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFP9896285.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLAEMAEAVLRQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRLPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFRHVLVALKQRV"
     gene            66029..66712
                     /gene="gntX"
                     /locus_tag="ACHOUK_000777"
     CDS             66029..66712
                     /gene="gntX"
                     /locus_tag="ACHOUK_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFP9896286.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAITRTRAT
                     ATQHFLSARLRRRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            66771..67346
                     /gene="nfuA"
                     /locus_tag="ACHOUK_000778"
     CDS             66771..67346
                     /gene="nfuA"
                     /locus_tag="ACHOUK_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFP9896287.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            67707..69023
                     /gene="gntT"
                     /locus_tag="ACHOUK_000779"
     CDS             67707..69023
                     /gene="gntT"
                     /locus_tag="ACHOUK_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFP9896288.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(69068..71152)
                     /gene="malQ"
                     /locus_tag="ACHOUK_000780"
     CDS             complement(69068..71152)
                     /gene="malQ"
                     /locus_tag="ACHOUK_000780"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFP9896289.1"
                     /translation="MESKRLDNAALAAGISPHYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHITGGKAFNLPT
                     KLPEGYHTLTLTQEDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWQAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(71162..73555)
                     /gene="malP"
                     /locus_tag="ACHOUK_000781"
     CDS             complement(71162..73555)
                     /gene="malP"
                     /locus_tag="ACHOUK_000781"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFP9896290.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLKKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            74167..76872
                     /gene="malT"
                     /locus_tag="ACHOUK_000782"
     CDS             74167..76872
                     /gene="malT"
                     /locus_tag="ACHOUK_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFP9896291.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            76939..77217
                     /locus_tag="ACHOUK_000783"
     CDS             76939..77217
                     /locus_tag="ACHOUK_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFP9896292.1"
                     /translation="MKPESKEAPINIRAKASQRDLIDMAANLVAKSRTDFMLDAACRE
                     AQDILLDQRLFILDDEQYDAFLAALDAPITAERQAKINALMNRKSPWE"
     gene            77208..77693
                     /locus_tag="ACHOUK_000784"
     CDS             77208..77693
                     /locus_tag="ACHOUK_000784"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9896293.1"
                     /translation="MGITAPTPLTSEHNLADFCCSDHGMNEWLKKKALKNHSSGLSRV
                     YVICIANTRQVIGYYCLSTGSIQRNLAPGAMRRNAPESLPVVVLGRLAIDQAWAGKGL
                     GVALLKDAVYRTMSIAQQVGVRALIVHALDDSVRNFYLKYAFVPSPFQSLTLLYPITL
                     E"
     gene            complement(77743..78771)
                     /gene="rtcA"
                     /locus_tag="ACHOUK_000785"
     CDS             complement(77743..78771)
                     /gene="rtcA"
                     /locus_tag="ACHOUK_000785"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFP9896294.1"
                     /translation="MKRMIALDGAQGEGGGQIMRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAATEICGATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAASFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGQFTVAHPSCHLLTNIAVVERFLPVRFTLAETDGVTR
                     VMITKLTD"
     gene            complement(78775..80001)
                     /gene="rtcB"
                     /locus_tag="ACHOUK_000786"
     CDS             complement(78775..80001)
                     /gene="rtcB"
                     /locus_tag="ACHOUK_000786"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFP9896295.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDVMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKMFSVEDQIRATVHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
BASE COUNT        19101 a        21722 c        20284 g        18947 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 aatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcctgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccatacgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgagc acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggccggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcgaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtcgccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggggtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagatcca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctggca gtagctgaaa
     4081 aacgccatca gcaataaaga agacggccag ctcgtcagtt aacgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgcc cggcagcggt accatgaggc acagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tccctgctga aggtttgacg aagccagccc cagtcttccg
     4321 gcctccattt catcaacaac gccacggcgt aatgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca agtggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctggaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaagg tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctgctcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 accagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagagagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcacgacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagtggaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttcttttag agagttttcc
     6121 atgtaacgac ccagcgaggc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gctgctttgc tgtcagcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaagg caacggccat tgtggtcgcc agcagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctact ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccgc aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccacgacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcggt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc accaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gcgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tggtgtccgc
     7561 acttaacgca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcaa taaaacgttt tcggattgcc atctttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct agcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcggcat
     7801 gcattgggat gagctctcgc agcatccgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc ataccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatctacct gcgtggcatc accgtaataa actttgtagc
     8161 catatttgcg catcaggtta acggcgctga tatcccgctc cagcacagta atgcgcattt
     8221 tatttgccat caacaaacga ccaatcacct gcccaaaacg tccaaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcagcgg cgtggtcatc atggaaagcg
     8401 tcaccgtcac cagcaacagc gccatctggt cgccctggaa taagcgttgt gaagaagcgg
     8461 tagaaaagag gacaaaggca aactcaccgc cctgactcaa cacgccagca aactgcatcc
     8521 gctctgagct acgcacgcca tacaatcgcg ccagcagata cagcacgaga attttcaccg
     8581 ccaccagcac aaccacgctt atcactaccc acaacagatg ggtataaagc accccgaggt
     8641 tgagtgacat cccgacagag ataaagaaca aaccgagcag caagccttta aagggatcga
     8701 tagccgtttc cagttcatgg cgatattcac tttccgccag cagcacgcct gcaataaacg
     8761 taccgagcgc catcgacagc cccaacgcat ccataaacaa tgcggaaccc aataccagca
     8821 gcagcgtcgc ggcggtgaac acttcccgca cgccagaagc tgcaataaaa cggaataccg
     8881 gacgcaataa atagcgcccg ccaatcagca tgccgacaaa cgccagcacc ttcatgccga
     8941 tcttcatcca gtcgaaatgt tcgtctgccg aacccgccaa caacggcact aacgccagtg
     9001 ccgggatcac cgccagatcc tgaaacagca gtaccgaaaa cccgagctgc ccggattcgc
     9061 tgcgattcat ccctttctca cgcatcaatt gcaacgccat tgcggtcgaa gacatcgcaa
     9121 ggccaatgcc accgaccacc gccgcctgcc aggcgaaatc cgtcagcatc aataatcccg
     9181 ccagcaacgc cgcgcttaac agcacctgtg ccgcgcctac gccaaaaatc gaacgccgca
     9241 gttgccaaag cttggaggga ttcaactcaa ggccgatgat aaacatcagg aacaccacgc
     9301 cgagttccga aaagtggagg atctcatcga cgtcgctaat aaaccccagc ccccacgggc
     9361 caattgcaat ccctgccagc aaatatccca acacagcgcc aatacccagc cgcgatgcca
     9421 acggcaccgc agccaccgcc gcgaagagaa acagcactcc tgcaagtaaa aaatcggaac
     9481 cttccatcag cggcctcctg gagacagcgg atttgccagc cagtcaccgt aggctctggc
     9541 gtggctcgcc agctcctgtg cgctttgccg tctcgcccag taaataatga tgggacttaa
     9601 ccaatgcatc cggcacatgc ccgccgccag ttcaaagggg cgcaggacat cgctcatcgg
     9661 gtaacgattc aacgcgtcat aacggtaagc actttccggc tcgccggtgg taatcacact
     9721 acgccagtac tttcccgcca gttggtttcc tcccggcccg ctggcaaaac cacggcttaa
     9781 tacccggtcc agccactctt tcagtagcgc cgggcagcta taggtataaa gaggatgctg
     9841 aaagacaatc acctcgtgct cgcgcagtaa tgcctgctca cgggggatat caataaaaaa
     9901 atcgggatag tgcgcgtaaa ggtcgtgcac ggtaacattg ctgagctgcg tggccggttt
     9961 aagcagtacc cggtttgcca ccgagtcctg agattccgga tgggcataca gcagcaaaac
    10021 tttcgctggc tgagacatca tccccctccc ggtatggttt ttgtgtatag tcgctgtttt
    10081 gggctaccat tgcgcccggt gcggcagctc gcccatacat tacattatca taatgataag
    10141 ttaacatagt ctgaacatac ggcaccttat gattgttttc tcctcgttac aaattcgtcg
    10201 cggcgtgcgc gtcctgctgg ataatgccac cgccaccatc aaccccgggc agaaagtcgg
    10261 cctggtgggt aaaaacggct gtggtaaatc taccctgctg gcattgctga aaaatgaaat
    10321 cagcgccgac ggcggcagct acacctttcc gggaagctgg caactggcgt gggtgaatca
    10381 ggaaacgccg gcgttaccgc aagcggcgct ggaatatgtc attgacggcg accgtgaata
    10441 tcgtcaactg gaagcgcagc tacacgacgc caacgaacgt aacgacgggc acgccattgc
    10501 gaccattcat ggcaagctgg atgctattga cgcatggagt attcgctccc gtgccgccag
    10561 cctgctgcac ggcctcggtt tcagcaatga acaactggag cgcccggtaa gtgatttctc
    10621 tggtggctgg cgtatgcgtc ttaaccttgc ccaggcgctg atttgccgtt cagacttgct
    10681 gctgctcgac gaaccgacta accacctcga tctcgatgcc gttatctggc tggaaaaatg
    10741 gctgaagagc tatcagggca cgctgatcct gatctctcac gaccgcgact tcctcgatcc
    10801 gatcgtcgat aaaattattc atatcgaaca acaaagcatg ttcgagtaca ccggcaacta
    10861 cagttcgttt gaagtacagc gcgccacccg tctggcgcag caacaagcga tgtacgaaag
    10921 ccagcaggaa cgcgtagcgc atctgcaaag ttatatcgac cgtttccgtg ccaaagccac
    10981 caaagcgaag caggcccaga gccgcattaa gatgctcgag cgtatggagc tgattgcccc
    11041 cgcgcacgtc gacaacccgt tccgctttag cttccgcgcg ccggaaagcc tgccaaatcc
    11101 gttactgaag atggaaaagg tcagcgcagg ctatggcgat cgcattattc tcgactcgat
    11161 taaactgaat ctggtccccg gttcgcgcat tggtctgtta ggccgcaacg gcgcgggtaa
    11221 atcgacatta atcaaactgt tagccggtga acttgcgcca gtcagtggtg agataggcct
    11281 ggcaaaaggg atcaagctcg gctacttcgc ccagcatcaa cttgaatacc tgcgcgccga
    11341 cgaatcgcct attcaacatc tggcacgttt agcgccgcag gaactggagc aaaaactgcg
    11401 tgactacctc ggcggctttg gtttccaggg cgataaagta accgaagaaa cgcgccgctt
    11461 ctccggtggg gaaaaagccc gcctggtgct ggcattaatc gtctggcagc gtccgaatct
    11521 gctgctgctc gacgaaccga ctaaccacct tgaccttgat atgcgtcagg cactcaccga
    11581 agcattaatc gagtttgaag gcgcgctggt tgtcgtttcg cacgaccgtc atttgctgcg
    11641 ttccaccact gacgatctct acctggttca cgatcgtaaa gtcgaaccgt tcgacggcga
    11701 tctggaagat tatcaacagt ggttgagcga cgtacaaaag caggaaaacc agaccgacga
    11761 agcgccaaaa gagaacgcga acagcgccca ggcacgtaaa gatcagaagc gtcgggaagc
    11821 ggagctgcgt gcgcaaaccc agccactgcg taaagagatt gcccgtctgg aaaaagagat
    11881 ggagaagctg aacgcgcaac tggcgcaggc agaagagaaa ctcggcgaca gcgaactgta
    11941 tgaccagagc cgtaaagcgg agttgaccgc ctgcctgcaa cagcaagcca gcgccaaatc
    12001 cggcctggaa gagtgcgaaa tggcatggct ggaagcccag gagcagcttg agcagatgct
    12061 gctggaaggc caaagcaact gatggcgcag ataacgacga ccgatgccaa tgaattcagc
    12121 agcagtgctg aattcacccc tatgcgcggc tttagcaatt gtcatctgca aaccatgctg
    12181 ccgcgtctgt ttcgtcgcaa ggtgaaattc accccgtact ggcagcgact ggagttgccc
    12241 gacggcgatt ttgtcgatct cgcgtggagt gaagcccctg cacaggcgca acataaacct
    12301 cgtctggtgg tgtttcacgg gctggaaggc agtctcaata gcccttacgc ccacggtctg
    12361 gttgaggcgg cgcaaaagcg cggctggctg ggcgtggtga tgcattttcg cggatgcagc
    12421 ggtgaaccaa accgtatgca ccgcatttac cattcgggcg aaaccgaaga cgccagttgg
    12481 tttttacgct ggctgcaacg cgagtttggc catgcgccaa cggctgccgt cggctattcg
    12541 ctcggcggta atatgctggc ctgtttgctg gcaaaagaag gcaatgatct cccggttgat
    12601 gcggcggtta ttgtctctgc gccgtttatg ctggaagcct gtagctatca tatggaaaag
    12661 ggcttttccc gcgtttatca gcgttacttg ctgaacctgt taaaagccaa tgccgcgcgc
    12721 aagctggcag cctaccccgg aacactgccg attaatctcg cgcagttaaa atcggtacgt
    12781 cgcatccgtg aatttgacga tctcatcacc gccagaattc acggctacgc tgacgctatc
    12841 gactattatc gtcagtgtag cgccatgccg atgctgaacc ggatcgccaa accgacgctg
    12901 attattcacg ccaaagacga tccgtttatg gatcatcagg tgatcccgaa accggaaagt
    12961 ctccccccgc aggtggagta tcaactgact gaacatggcg gtcatgttgg ctttattggc
    13021 ggtacattac ttcatccgca aatgtggctg gagtcacgca ttcctgactg gttaacaacg
    13081 tatctggagg cgaaatcatg ttgattccgt ggcaagacct ctcccccgaa acgctggaaa
    13141 atttaattga aagctttgtg ttacgtgaag gcaccgatta tggtgaacat gagcgtacac
    13201 ttgaacagaa agtcgccgac gtcaaacgcc agctacagtg cggagaagcg gtgctggtat
    13261 ggtcggagct gcacgaaacg gtcaatatca tgccgcgcag tcagtttcgc gaataacctg
    13321 caaccgtcgg cggacgagat aataatgata gctaagtcat ggagttacta tgtctgccaa
    13381 acatccggtc attgcggtaa caggatccag cggcgcgggg accaccacca ccagcctcgc
    13441 gtttcgtaaa atattcgcgc agttaaatct gcatgcagcc gaggtggaag gcgacagttt
    13501 tcaccgttac acccgcccgg aaatggacat ggcgatccgc aaagcgcgcg acgccgggcg
    13561 gcatatcagc tacttcggcc ccgaagcgaa cgacttcggc ctgctggaac aaaccttcat
    13621 tgaatacggt cagagcggca aagggaaatc tcgcaaatat ctgcatacct acgatgaagc
    13681 cgtaccgtgg aatcaggtac cgggaacatt caccccctgg caacctttac cagaacccac
    13741 tgatgtactg ttttatgaag gtttacacgg cggcgtagtc acgccacagc ataacgttgc
    13801 gcagcatgtg gacttactgg tcggcgtggt acctatcgtt aaccttgagt ggattcaaaa
    13861 actgatccgc gacaccagcg agcgcgggca ctcacgagaa gcagtgatgg attcagtagt
    13921 gcgttcaatg gaagactata tcaactacat cacaccgcag ttttcccgca cccatcttaa
    13981 cttccagcgc gttcccaccg tcgacacttc aaacccgttc gcggcaaaag gtatcccgtc
    14041 gctggatgaa agctttgtgg tgatccattt tcgtaatctg gaagggatcg atttcccctg
    14101 gctgctggcg atgttgcaag gctcattcat ttcccacatc aatacgttag tggtgcccgg
    14161 cggcaaaatg ggtctggcaa ttgaattaat tatgctgccg ctggtgcaac gattgatgga
    14221 aggaaagaaa atcgagtaac tctgctatta cgccggataa aatactatcc ggcttcacaa
    14281 cgggatagtt aaatcacgcg gcaaccactt catacgagtg agtaatattc accgcttttt
    14341 ccagcatcaa cgccactgaa caatatttct cagcagagag atcaaccgca cgcgcaaccg
    14401 ctgcgtcttt caggtcgcga ccggtgacga taaaatgcag attaatgtgc gtaaacaggc
    14461 gtggtgcctc ttcgcggcgt tcagaggtca attttacttc acaatcgacc acatcctgac
    14521 gccctttttg caggatcgaa accacatcga tggcactgca accacccgcc gccatcaaca
    14581 ccatttccat cggacttggt gctttatcgc ctgagttgcc gtccattaaa atctggtgac
    14641 cagaggcaga ttcgcccaga aaagttaacc cttcgaccca cttcactcgc gcttgcattt
    14701 ttgctactcc actgcgtcaa ttttcctgac agagtacgcg tactaaccaa atcgcgcaac
    14761 ggaaggcgac ctgggtcatg ctgaagcgag acaccaggag acacaaagcg aaagctatgc
    14821 taaaacagtc aggatgctac agtaatacat tgatgtactg catgtatgca aaggacgtca
    14881 cattaccgtg cagtacagtt gatagcccct tcccaggtag cgggaagcat atttcggcaa
    14941 tccagagaca gcggcgttat ctggctctgg agaaagctta taacagagga taaccgcgca
    15001 tggtgcttgg caaaccgcaa acagacccga ctctcgaatg gttcttgtct cattgccaca
    15061 ttcataagta cccatccaag agcacgctta ttcaccaggg tgaaaaagcg gaaacgctgt
    15121 actacatcgt taaaggctct gtggctgtgc tgatcaaaga cgaagagggt aaagaaatga
    15181 tcctctccta tctgaatcag ggtgatttta ttggcgaact gggcctgttt gaagagggcc
    15241 aggaacgtag cgcatgggta cgtgcgaaaa ccgcctgtga agtggctgaa atttcgtaca
    15301 aaaaatttcg ccaattgatt caggtaaacc cggacattct gatgcgtctg tctgcacaga
    15361 tggcgcgtcg tctgcaagtc acttcagaga aagtgggcaa cctggcgttc ctcgacgtga
    15421 cgggccgcat tgcacagact ctgctcaatc tggcaaaaca accagacgct atgactcacc
    15481 cggacggtat gcaaatcaaa attacccgtc aggaaatcgg tcagattgtc ggctgttctc
    15541 gtgaaaccgt gggacgcatt ctgaagatgc ttgaagatca gaacctgatc tccgcacacg
    15601 gtaaaaccat cgtcgtttac ggcactcgtt aatcccgtcg gagtggcgcg ttacctggta
    15661 gcgcgccatt ttgtttcccc cgatgtggcg cagactgatt tatcaccccg atattaacta
    15721 tgcactgcga caaacgctgg tgctatgttt gcccgtggcc gttgggttaa tgcttggcga
    15781 attacgattc ggtctgctct tctctctcgt tcctgcctgt tgcaatattg cgggccttga
    15841 tacgcctcat aaacgttttt tcaaacgctt aatcattggt gcgtcgctgt ttgccacctg
    15901 tagcttactg acacagctgc tactggcaaa agatgttccc ctgccttttt tgctgaccgg
    15961 attaacgctg gtacttggcg tcactgctga gttggggcca ttgcacgcaa aattgcttcc
    16021 cgcatcgctg ctggcagcca tttttaccct cagtctggcg ggatacatgc cggtctggga
    16081 accgttgctc atctatgcgt tgggcactct ctggtacgga ttgtttaact ggttttggtt
    16141 ctggatctgg cgcgaacaac cgctgcgcga gtcactaagt ctgctgtacc gtgaactggc
    16201 agattattgt gaagccaaat acagcctgct tacccagcac accgaccctg aaaaagcgct
    16261 gccgccgctg ctggtgcgcc agcaaaaagc ggtcgatcta attacccagt gctatcagca
    16321 aatgcatatg ctttccgcgc aaaataatac tgactacaag cggatgctgc gtattttcca
    16381 ggaggcgctg gatttacagg aacatatttc ggtcagtttg catcagccgg aagaggtgca
    16441 aaagctggtc gagcgtagcc atgcggaaga agttatccgc tggaatgcgc aaaccgtcgc
    16501 cgctcgcctg cgcgtgctgg ctgatgacat tctttaccat cgcctgccga cgcgttttac
    16561 gatggaaaag caaattggcg cactggaaaa aatcgcccgc cagcatccgg ataatccggt
    16621 tgggcaattc tgctattggc acttcagccg catcgcccgc gtgctgcgca cccaaaaacc
    16681 gctctatgcc cgtgacttac tggccgataa acagcggcga atgccgttac ttccggcgct
    16741 gaaaagttat ttgtcactaa agtctccggc gctacgcaat gccggacgac tcagtgtgat
    16801 gttaagcgtt gccagcttga tgggcaccgc gctgcatctg ccgaagtcgt actggatcct
    16861 gatgacggta ttgctggtga cgcaaaatgg ctatggcgca acccgtctga ggattgtgaa
    16921 tcgctccgtg ggaaccgtgg tcgggttaat cattgcgggc gtggcgctgc actttaaaat
    16981 tcccgaaggt tacaccctga ccttgatgct gattaccacc cttgccagct acctgatatt
    17041 gcgcaaaaac tacggctggg cgacggtcgg ttttactatt accgcagtgt ataccctgca
    17101 actgttgtgg ctgaacggtg agcagtacat ccttccgcgt cttatcgata ccattattgg
    17161 ttgtttaatt gctttcggtg gtaccgtctg gctgtggccg cagtggcaga gcgggttatt
    17221 gcgtaaaaac gcccatgacg ctttagaagc ctatcaggaa gcgattcgct tgattctgag
    17281 cgaggatccg caaccaacgc cactggcctg gcagcgaatg cgggtaaatc aggcgcataa
    17341 cactctgtat aactcattga atcaggcgat gcaggaacca gcgttcaaca gccattatct
    17401 ggcagatatg aaactgtggg taacgcacag tcagtttatt gttgaacata ttaatgccat
    17461 gaccacgctg gcgcgggaac accgggcatt gccacctgaa ctggcacaag agtatttaca
    17521 gtcttgtgaa atcgccattc agcgttgtca gcagcgactg gagtatgacg aaccgggtag
    17581 ttctggcgat gccaatatca tggatgcgcc ggagatgcag ccgcacgaag gcgcggcagg
    17641 tacgctggag cagcatttac agcgggttat tggtcatctg aacaccatgc acaccatttc
    17701 gtcgatggca tggcgtcagc gaccgcatca cgggatttgg ctgagtcgca agttgcggga
    17761 ttcgaaggcg taatgcaggc taaaccatcg gataaggcat gcacgccgca tccgacatct
    17821 tttgcctgat gcgtcttatt tacgccccaa ccaccttcgc caccgcgtgg gcgaaacgtt
    17881 gcatcccttc atcgatatcc gcatcttcca ccaccagcga cggcgcaaaa cgcatcacat
    17941 ccggtccggc attcagcacc attacgccag cctctgcgcc cgcatacagg aaatcacgcg
    18001 cctgaccttt gtactgtggt ttcagctccg cgccaattaa cagccccata ccgcgaatat
    18061 cgctaaacac atcgtactgc tgatcgatct tctgcaaatg gtcaacaaaa cgctggcgtt
    18121 tcgcctgaat gccttccagc acttcagggg tattaatgat atcaaacgcc gcgcccgcta
    18181 ctgcacaggc cagaggatta ccgccgtagg tggaaccgtg agaaccagga tgaaacgcag
    18241 aagcaatttc cgccgtggtc agcatggcgc taatcgggaa gccgccgcct aacgctttcg
    18301 cagaagtcag aatatccggc gtcacgccgt agtgcatgta agcaaacaaa tcgccagtcc
    18361 gccccatccc gcactgtact tcatcaaaca ccaataatgc ctgatgttga tcgcacaact
    18421 cacgcaagcc ctgcaaaaac tctggcgtcg ctgccgtcac accgccctcg ccctggatcg
    18481 gctcaaccac caccgcacag gtgtgatcat ccatcaccgc tttcactgcg tggagatcgt
    18541 taaagggaac gtggatgatg tctgatggtt ttggcccaaa gccgtcggaa tattttggct
    18601 gcccacccac cgaaacggta aacagcgagc gaccatgaaa agcgttatgg aaggcaataa
    18661 ttttggtttt gaacgggcta tgacgcacgc aggcgtaatg gcgtgccagt ttaaaggcgg
    18721 tttcgttagc ttccgtgccg gagttcataa acaccacgcg ttcggcaaac gtcgcctcaa
    18781 tcagtttacg cccaagacgc agcgccggtt cattggtgaa aacgttactg atatgccaca
    18841 gagtttcgcc ctgggttttt aacgcgttca ccagcgcagg atggcaatgg cccaacgccg
    18901 taactgcaat gccacccgcg aaatcgacat actccttgcc ttgctgatcc cagattcggc
    18961 tgccctgacc ttttaccgga atgaactctg ccggagcata aatcggcagg atcacttcat
    19021 cgaaagtcgc gcgtgtaatt gctgtttgtt caattgccat ctcatgatca ccctgttatg
    19081 cataaataaa tgtgaaatta taatcacaaa atatgcataa aaaatcattg aatggcaatc
    19141 agaaatcagc gatgcaggaa attagccagc agttgatgtc cttgttcgct aagaatactt
    19201 tctggatgga actgcacacc ttccagatcc cactggcgat ggcgaatccc cataatctcg
    19261 cgggtttcgc tccaggccgt cacttcaaag cacgctggta acgagtcagg ttccactacc
    19321 agcgaatgat agcgtgtcac ggtaagtgga tttgccagcc cccgaaatac gccctcaccg
    19381 ttatgtgtaa tcggcgaggt tttgccgtgc atgacctttg cggcgcgcac aactttaccg
    19441 ccaaatgcct gcgccattgc ctgatgaccg aggcagacgc caagaatcgg caagcgcccg
    19501 gcatagtggc gaataacgtc aagggagatc ccggcttcat ctggcgtaca ggggccgggt
    19561 gagatgacaa ctttttgtgg tttaagggcg tcgatatccg ccagcgtcaa cgcatcgttg
    19621 cgcttaacca gcacatccgc ccccagttca caaaagtact ggtagaggtt ccaggtaaaa
    19681 gaatcgtagt tatctataag caggatcatg gcggctccgg tacgaaagaa ccgcgctatt
    19741 ttactcagat tccccggctt cgcttaccac tttgctaaat atcatctgca acgcggtgag
    19801 atcccccatt gccccactct gatttgcctg attccaggcc tctttttcga taccctgcca
    19861 gctcagttga tatccggcat gaatcgccag ttgctcgaag aagatccgct gtgccagacc
    19921 acttcccacc cggaagggat gcagcacgtt gatttcacaa tagtaatgcg ccagccgctc
    19981 gacgaacttc gttttctcca ggccaaccag ataaccttct tcctccagat cctgcatcag
    20041 ggcattgccc tctttttcga tataagcaaa gtggcagaac ggcgtatcgc cctgataaat
    20101 atcaacttca cgcagttgcc ctgcccagtc gaaaatatcc tgatacagct ggcgatggat
    20161 agtgcgcaaa tgcggtaaac cgcgcaccag cggaccaagc tcaatggtcg cagcacgcag
    20221 cgccgtcatt tcgtaagcgg cctgttccag ccgttgttgc tggcggatgt tcagccggtt
    20281 acgcatgata tcaaggccag gataaagata cggatcgcgc ccttcgccga atttatcgct
    20341 catagtgact cctcagctct tcaaggcgcg ctaaagcctc tgcggcagta agagtgacta
    20401 aaggcatctc gacgccttca agacggcgac tggcctgaaa attacgatta cgctgaagct
    20461 cccagagacg ggacttttgc ttatcggtga gtttcttcac ttgatgcctc cctgaacgtg
    20521 tccatttgcc acaagtataa gcggcaaatc cgggttacgc cgggagagca gaagcgcggg
    20581 caagattgcc cgcgcgagaa atcattacgg caggacttta gcggaaagga taactaccgg
    20641 ttttgacggc acattctggt acggaccaac gtcatgagtc ggcacctggg aaatcttatc
    20701 ggcaacgtcc atgcctttca ccactttacc aaataccgcg taaccgaaat cacgctgacc
    20761 atggtcaagg aaggcgttat cggcaacgtt gataaagaac tggctggtgg cgctgtcttt
    20821 gtcagcggta cgcgccatcg cgatggtgcc acgcgtgttg cgaaggccgt tatcggcttc
    20881 atttttgatt ggcgggtttg gttttttctg ctgcatctgc tcggtgaaac cgccgccctg
    20941 aatcataaag ccaggaatga cgcggtgaaa ggtagtgttg ttataaaaac cgctgttcac
    21001 ataatcgaca aagttttgca cagacactgg cgctttttgt ttatccagct ccagttcgat
    21061 gttaccagct gaggttgtca acaatacgtg cgggtcccct ttcgctgcca ttgctgcggg
    21121 agaaagagca gaaagagcga aaacagcagc catcgccgcc agggtcgatt tgaacatgag
    21181 atttccttaa caaacagagc agaaaaaaag caagtgaaga gattctaaat agccttcaag
    21241 aacaaggcca tccctttacc taattttacg tatctgaaat atatgtaacc tgacgatcac
    21301 tttttattcc gtgatcaaaa tcacctctta aaatgcaatt tagcaaccga ttgcaataaa
    21361 acatttaaac agatcacaaa atcacctaaa atcgcccgct cgcggcatat cccgctatgc
    21421 cattttattt ttgaacgcag gccaatcatg actaacagca atcgcatcaa gctcacatgg
    21481 attagctttc tctcctacgc actgactggt gcgttggtta ttgtcaccgg gatggtgatg
    21541 ggaaatatcg ccgattattt caatctgcct gtttccagta tgagtaacac cttcaccttc
    21601 ctcaacgccg gcattttaat ctctatcttc ctcaacgcct ggctgatgga aatcgtcccg
    21661 ttgaaaacgc agttacgttt tggctttctc ctgatggtgc tggcggttgc cggtttgatg
    21721 ttcagccaca gcctggcgct gttctcggcg gcgatgttca ttctcggggt ggtcagcggc
    21781 atcaccatgt cgattggtac attcctggta acacaaatgt atgaagggcg tcagcgcggt
    21841 tcccgcctgt tatttaccga ctccttcttc agtatggctg ggatgatttt cccaatgatc
    21901 gccgcgtttc tactggcgcg cagcattgag tggtactggg tttatgcctg catcgggctg
    21961 gtgtatgtcg ctatttttat tctgaccttc ggctgtgagt tcccggcgct gggcaaacat
    22021 gcaccaaaaa cggatgctcc ggtagcgaaa gaaaagtggg ggatcggcgt actgtttctc
    22081 tctgttgcgg cgctgtgcta catcctcggt cagttaggtt ttatctcctg ggtgcctgag
    22141 tatgccaaag gcctgggcat gagcctgaat gatgctggca cgctggtgag taacttctgg
    22201 atgtcataca tggtcggcat gtgggcgttc agctttattc ttcgcttctt tgatttgcaa
    22261 cgcattctga ccgtactggc tggtctggct gcgattctga tgtacgtctt taacaccgga
    22321 acaccggcac atatggcgtg gtcaattctc gccctgggct tcttctccag cgcgatctat
    22381 accaccatca tcaccctggg ttcacagcag accaaagtac cgtcgccaaa actggttaac
    22441 tttgtcctga cctgcggaac catcggtact atgttgacct ttgtggttac cggcccgatt
    22501 gttgaacata gcggtccgca ggcggcactg cttacagcaa acggtctgta cgctgtcgtc
    22561 tttgtgatgt gcttcctgtt aggtttcgtc agccgtcacc gtcagcataa caccctgacc
    22621 tctcattaat tgctcatgcc ggacggcact atcgtcgtcc ggccttttcc tctctaaccc
    22681 cgctacgtgc atctatttct ataaacccgt tcattttgtc tattttttgc acaaacatga
    22741 aatgtcagac aattccgtga cttaagaaaa tttatacaaa tcagcaatat accccttaag
    22801 gagtatataa aggtgaattt gatttacatc aataagcggg gttgctgaat cgttaaggta
    22861 ggcggtaata gaaaagaaat cgaggcaaaa atgagcaaag tcagactcgc aattatcggt
    22921 aacggtatgg tcggccatcg ctttatcgaa gatcttcttg ataaatctga tgcggccaac
    22981 tttgatatta ccgttttctg tgaagaaccg cgcatcgctt atgaccgcgt acacctctcg
    23041 tcttacttct ctcaccacac cgccgaagag ctgtcgctgg tgcgcgaagg cttctacgag
    23101 aaacacggca tcaaagttct ggtcggcgaa cgcgctatca ccatcaaccg tcaggagaag
    23161 gtgattcact ccagcgccgg acgtaccgtt ttttatgaca agctgatcat ggcgaccggt
    23221 tcctacccgt ggatcccgcc aattaaaggt tctgatactc aggactgctt tgtctatcgc
    23281 accattgaag acctcaacgc cattgaatcc tgcgcccgtc gcagcaaacg cggtgccgtt
    23341 gttggtggcg gcctgttagg tctggaagcc gctggcgcgc tgaaaaactt aggtattgaa
    23401 acccacgtta tcgaatttgc ccctatgctg atggcagaac agcttgatca gatgggtggc
    23461 gagcagctgc gtcgcaaaat cgagagcatg ggcgtgcgcg ttcacaccag caaaaacacc
    23521 cttgagattg tgcaggaagg tgttgaagcg cgtaaaacca tgcgttttgc cgacggcagc
    23581 gaactggaag tcgactttat cgtcttctct accggtatcc gtccgcgcga taagctggca
    23641 acccagtgtg gtctggacgt tgctccgcgt gggggtattg tcattaatga ttcctgccag
    23701 acttccgatc cggatatcta cgccatcggt gaatgcgcaa gctggaacaa ccgtgtattt
    23761 ggtctggtag caccaggcta caaaatggcg caggtcgccg ttgaccatat tctcggtagc
    23821 gaaaacgcct ttgaaggtgc tgaccttagc gccaagctga aactgctggg cgtagacgta
    23881 ggcggtattg gtgatgcgca cggtcgcacg cctggcgcac gtagctacgt ttacctcgac
    23941 gaaagcaaag agatctacaa acgccttatt gtcagcgaag acaacaaaac cctgctcggt
    24001 gcggtactgg tgggcgatac cagcgactac ggcaacctgc tgcaactggt gctgaatgct
    24061 atcgagctgc cggaaaaccc ggattctctg attctgccgg cgcactcggg tagcggtaag
    24121 ccgtctatcg gtgttgataa actgccggac agcgcgcaaa tctgctcctg cttcgacgtc
    24181 accaaaggtg atctgattgc tgccatcaac aaaggctgtc acaccgttgc ggcgctgaaa
    24241 gccgaaacca aagcgggtac tggctgcggt ggctgtatcc cgctggtcac tcaggtactg
    24301 aacgcggaac tggcgaaaca gggcatcgaa gttaacaaca acctgtgcga acactttgct
    24361 tattcgcgtc aggaactatt ccatttgatc cgcgttgaag gcattaaaac cttcgaagaa
    24421 ctgctggcga aacacggcaa aggttacggt tgtgaagttt gtaaaccgac cgtcggttcg
    24481 ctgctggcct cctgctggaa cgaatacatt ctgaagccgg aacatactcc gctgcaggat
    24541 tctaacgaca acttcctcgc taacatccag aaagacggca cctactcggt gatcccgcgc
    24601 tctccgggcg gtgaaatcac cccggaaggg ctgatggcgg taggtcgtat cgcgcgtgaa
    24661 tttaatctct acaccaagat cactggctcc cagcgtctgg cgatgtttgg cgcacagaaa
    24721 gacgatctgc cggagatctg gcgtcagctg attgaagccg gcttcgaaac cggtcatgcc
    24781 tatgcgaaag cactgcgtat ggcgaaaacc tgcgtgggta gcacctggtg ccgctacggc
    24841 gttggcgaca gcgtcggcct cggcgtggaa ctggaaaacc gctacaaagg catccgtacg
    24901 ccgcacaaaa tgaagttcgg tgtctccggc tgtacccgtg aatgttcaga agctcagggt
    24961 aaagacgtgg gtattatcgc cactgaaaaa ggctggaacc tgtatgtttg cggtaacggc
    25021 ggcatgaaac cgcgtcatgc ggatctgctg gcggcggata tcgatcgcga aacgctgatc
    25081 aaatatctcg accgcttcat gatgttctac atccgtactg ccgacaaact gacgcgtacc
    25141 gcaccgtggt tagaaaacct cgaaggcggc atcgattacc tgaaagcggt gatcattgac
    25201 gacaaactgg ggctgaacgc acatctggaa gaagagatgg cgcgcctgcg tgaagcggta
    25261 gtgtgtgagt ggactgaaac ggtcaatacg ccgtctgcgc agactcgctt caaacacttc
    25321 atcaacagcg acaagcgtga cccgaacgtg cagatggtgc cagagcgcga acagcaccgt
    25381 ccggcaacgc cgtatgaacg tatcccagta actctggtgg aggacaacgc atgagccagt
    25441 ggaaagacat ctgcaaaatc gatgacatcc tgcctgaaac cggcgtctgc gcgctgttag
    25501 gtgacgagca ggtcgcgatt ttccgcccgt atcacagcga tcaggtgttt gcgatcagca
    25561 acatcgaccc gttcttcgag tccagcgtgc tgtcacgcgg actgattgcg gaacaccagg
    25621 gcgagctgtg ggtcgccagc ccgctgaaaa aacagcgttt tcgcttaagc gacggcttgt
    25681 gcatggaaga cgaacagttt tccgtcaaac attacgaagc gcgagtgaaa gacggcgtgg
    25741 tgcagctgcg cggttaatgt tttaacggga ggcgcaatgc ctcccctttt tgcatggtcc
    25801 tgtaataacc ttcggtatat tgcaggacat tttttaaact ttttgtttta ttttttgttt
    25861 ttatttttta aaggataatc aaatgtttac agacactatt aataagtgtg cggctaacgc
    25921 tgcgcgcatt gcacgcctgt cggcaaataa cccgctcggc ttttgggtca gctccgccat
    25981 ggcgggcgcg tatgtgggtc ttgggatcat cctgattttt acgctcggta atttgctcga
    26041 tccatccgta cgccctctgg tgatgggcgc gacctttggt atcgccttaa cgctggtgat
    26101 tatcgccggt tctgagctgt tcaccggaca caccatgttc ctcacctttg gggtgaaagc
    26161 gggcaccatc agccacgggc aaatgtgggc aatcctgccg caaacctggc tgggtaacct
    26221 ggtcggttcc gtcttcgttg ccatgctcta tagctggggt ggtggtagcc tgctgccggt
    26281 agataccagc atcgttcact ccgtcgcgct ggctaaaacc actgcaccgg caatggtact
    26341 gttcttcaaa ggtgcattgt gtaactggct ggtttgtctg gcaatctgga tggcgctgcg
    26401 cactgaaggg gcggcgaaat ttatcgcaat ctggtggtgt ctgctggcat ttatcgcgtc
    26461 cggctacgag cactccatcg ctaacatgac gctgttcgcg ctctcctggt tcggcaacca
    26521 cagcgaagcc tacacgctgg cgggtattgg tcataacctg ctgtgggtga cgctgggtaa
    26581 tactttatca ggtgccgtat tcatgggatt gggttattgg tatgctacgc cgaaagcgaa
    26641 tcgtccggtt gcggacaaat ttaatcaaac tgaaacggct gccggttaat tactaagggg
    26701 tttttacgtg gatcatttgc ctatattttg ccaattacgc gatcgcgact gtctgattgt
    26761 cggcggtggt gatgtcgcgg aacgcaaagc aaggttgctg ttagacgcag gcgctcgctt
    26821 aacggtgaat gcattagcgt ttattccaca gttcaccgca tgggcagatg caggcatgtt
    26881 aaccctcgtc gaagggccat ttgatgaaag ccttctcgac acctgctggc tggcgattgc
    26941 agcgacggat gatgacgcgc ttaaccagcg cgtcagcgaa gccgctgaag ctcgtcgcat
    27001 cttctgtaac gtggtcgatg cgccgaaagc tgccagcttc attatgccgt cgattattga
    27061 ccgctcaccg ctgatggtgg cggtttcctc tggcggtact tctccggttc tggcgcgcct
    27121 gttgcgcgaa aaactcgaat cactgctgcc gctgcatctg ggccaggtag cgaaatacgc
    27181 cgggcagtta cgcggtcggg taaaacaaca gttcgccacg atgggtgaac gccgccgctt
    27241 ctgggaaaaa ctgttcgtta acgaccgtct ggcgcagtcg ctggcgaaca acgatcagaa
    27301 agccattact gaaaccaccg aacagttaat caacgaaccg ctcgaccatc gcggtgaagt
    27361 ggtgctggtt ggtgcaggtc cgggcgatgc cgggctgctg acactgaaag gtctgcaaca
    27421 aattcagcag gcagatgtgg tggtctacga ccgtctggtt tctgacgata ttatgaatct
    27481 ggtacgccgc gatgcggatc gtgttttcgt cggcaaacgc gcgggatacc actgcgtacc
    27541 gcaggaggag attaaccaga tcctgctgcg ggaagcgcaa aaaggcaaac gcgtggtgcg
    27601 gctgaaaggc ggcgatccgt ttatttttgg ccgtggtggc gaagagctgg aaacactgtg
    27661 caacgcgggt attccgttct cggtggttcc gggtattacc gcagcttctg gttgctcagc
    27721 ctattcgggt attccgctca cgcatcgcga ttatgcccag agcgtgcgct taattaccgg
    27781 acacttaaaa accggtggcg agctggactg ggaaaacctg gcggcagaaa aacagacgct
    27841 ggtgttctat atggggctga atcaggctgc gactattcag caaaagctga ttgaacacgg
    27901 tatgcctggc gaaatgccgg tagcaattgt cgaaaacgga acagcagtca cgcagcgcgt
    27961 gattgacggt acgctcacac aactgggcga actggcgcag caaatgaata gtccatcgct
    28021 aattattatt ggtcgggttg ttggcctgcg cgataaactg aactggttct ccaaccatta
    28081 atttaatcag ggccagagaa ttctggcctt tttcgtttac aactctcttt atatattcag
    28141 cagataaaaa tataactcat taacaaaaat catttgtttt tgccccgtta tattcaccgt
    28201 tacagcgact tacaaaatta agtcattcac tcactgacgc cgattaaata ttcctgtatc
    28261 gttcttaatg agcagtctac ttatattcat ataatcaatg aatattaatc aatatataat
    28321 acatagggaa tgtaaatgaa caaatttatt aaagttgcac tggtaggtgc agtactggct
    28381 acgttaactg catgtactgg tcatattgaa aaccgtgata agaactgctc ttacgactac
    28441 ctgctgcacc cagcaatttc tatttctaaa atcattggcg gttgcggtcc tactgcacag
    28501 taagtctctg acagaaatca gctaacgctg atttaattgc ctgacgccct cgggattacc
    28561 cgagggcgta tttgtttcta tatccttctt gctccccacc cgtaaaaata ttttcacttt
    28621 aatttcaatt ggataaccac attcatcagc cacagcgcga tcctgtgcga aattatgtga
    28681 tcttcctcca cattacataa catcatatgt tgttatattc atcatgcatt gtcatgtcac
    28741 cttttaaatg actgcaaact ctcccctaca acgtgttgga caagaaaaag gtatcgctat
    28801 gggaagccag gaactccaac gcaagctcgg attttgggcc gttcttgcaa tcgccgtcgg
    28861 gacaaccgtc ggctccggta tttttgtatc tgtgggtgaa gtggcaaaag cggcgggcac
    28921 gccgtggctg acggtgctcg catttgtcat cggcgggtta attgtgatcc cgcaaatgtg
    28981 cgtatatgcg gaactatcca ccgcttatcc ggaaaatggc gcagattatg tttatctgaa
    29041 aaatgccgga agccgaccgc tggctttcct ctccggctgg gccagcttct gggccaacga
    29101 tgcgccgtca ttgtcgatta tggcgctggc gattgtcagc aatcttggct ttttaacgcc
    29161 tatcgatccg ttgctcggta aatttatcgc cgccggatta attatcgcct ttatgctgct
    29221 gcacctgcgc tccgttgaag gcggcgcagc gtttcagacg ctaattacca tcgccaaaat
    29281 tatcccgttc actatcgtca ttggtcttgg gatcttctgg tttaaagcgg agaattttgc
    29341 cgcccctgct accactgcga ttggcgcaac gggcagcttt atggcgctgc tggcggggat
    29401 ctctgccacc agttggtcgt ataccggcat ggcctctatc tgttatatga ccggcgaaat
    29461 taaaaacccc ggcaaaacca tgccacgagc gctgattggt tcctgtctgc tggttctggt
    29521 gctctacacc ctgctggcgc tggtgatttc cggcctgatg cccttcgaca aactcgccaa
    29581 ttctgaaacg ccgatttccg acgccctgac ctggatcccc gcactcggca gcaccgctgg
    29641 gatctttgtt gccatcacgg cgatgatcgt cattcttggt tcgctttcca gctgcgtgat
    29701 gtaccagccg cggctggaat acgcgatggc gaaagacaac ctgttcttca aatgcttcgg
    29761 ccatgtgcat ccgaaataca acacgccgga tgtctccatc attttgcaag gggcgctggg
    29821 gatcttcttc atcttcgttt ccgatctcac cagcctgctg ggctatttca ccctggtaat
    29881 gtgtttcaaa aataccctca ccttcggctc catcatctgg tgtcgtaaac gcgacgatta
    29941 caaaccgctg tggcgtactc cggctttcgg gctgatgacc cccctcgcca ttgcgtcaag
    30001 cctcattctg gtcgcctcaa cctttgtctg ggcaccgatt cccggcctta tctgcgccgt
    30061 catcgttatt gccactggtc tgcctgctta cgccttctgg gcgaagcgta gccgccagct
    30121 caacgctttg tcgtaatgtt atctggagaa aataaaatgt tggatattga taaaagcact
    30181 gtggactttc tggtcaccga aaatatggtt caggaagtgg aaaaagttct cagccatgac
    30241 gttccgctgg tgcacaccat cgtggaagag atggtgaagc gcgacatcga tcgtatttat
    30301 ttcgttgcct gcggatcgcc actcaacgcg gcgcaaacgg cgaaacatct ggcagatcgc
    30361 ttttccgatc ttcaggttta cgccatttcc ggctgggagt tctgcgataa caccccatat
    30421 cgcctcgacg ctcgttgcgc ggtgattggc gtttctgact acggtaaaac cgaagaggta
    30481 atcaaagcgc tggagctggg ccgggcctgc ggcgcactca ctgcggcgtt caccaaacgc
    30541 gcggatagcc cgattacctc ggcggcggaa tttagcattg attatcaggc cgactgtatc
    30601 tgggaaattc atctgctgct ctgctacagc gtggtgctgg agatgatcac ccgcctcgcg
    30661 ccgcacgcgg aaatcggcaa gatcaaaaac gatctcaaac agctgccgaa tgcgctcggt
    30721 tatctggtac gcacttggga agaaaaaggc cgccagcttg gtgaactggc cagccagtgg
    30781 ccgatgattt acacggttgc tgcaggcccg ctgcgcccgc tgggttacaa agaaggcatc
    30841 gtgacgctga tggaatttac ctggacgcac ggctgtgtga ttgagagcgg tgagttccgt
    30901 cacggcccgc tggagattgt cgaaccgggc gttccgttcc tgttcctgct cggcaatgat
    30961 gaaagtcgcc acaccaccga acgcgccatt aactttgtta aacagcgtac tgacaacgtg
    31021 atcgtcatcg attacgccgc aatttcgcaa ggactgcacc cgtggctggc accgttcctg
    31081 atgttcgtgc caatggagtg gctctgctac tacctgtcta tttacaaaga tcacaacccg
    31141 gatgaacgcc gctattacgg tggtctggtg gaatattaat cctctctccc ggcccggcaa
    31201 cgggccggat ttacgcaagg agttacccga tgaaaacagg tatgtttacc tgcggccacc
    31261 agcggctgcc tattgaacat gcattccgtg atgcaagcga gctgggttac gatggcatcg
    31321 aaatttgggg cggccgcccg cacgcgttcg cgccggactt aaaagcgggc ggcatcaaac
    31381 aaatcaaggc gctggcgcag acgtatcaga tgccgattat cggttacacg ccagaaacca
    31441 acggctatcc gtataacatg atgctgggcg atgaacatat gcgtcgtgaa agcctggaca
    31501 tgatcaagct ggcgatggat atggcaaaag agatgaacgc gggttatacg ctgatttctg
    31561 cggcccacgc gggctatctc acgccgccta acgttatctg ggggcggctg gcagagaact
    31621 taagcgaact gtgtgagtac gcggaaaaca tcggcatgga tctgatcctc gaaccgttaa
    31681 cgccgtatga atcgaacgtc gtgtgtaatg ctaatgatgt gcttcatgcg ctggcgctgg
    31741 tgccttcgcc gcgcctgttc agcatggtcg acatttgcgc gccgtatgtc caggcggaac
    31801 cggtgatgag ttatttcgac aaactgggcg ataaattacg tcatctgcat attgtcgaca
    31861 gcgacggggc cagcgacacg cattacattc ccggcgaagg caaaatgccg ctgcgggaac
    31921 tgatgcgcga tattattgat cggggctatg agggatactg cacagtggaa ctggtaacga
    31981 tgtatatgaa cgagcccaga ctttacgccc gccaggcgct ggaacgcttt cgcgcgctgc
    32041 tgccggagga tgagtgatga aaaccctggc gacaatcggc gataactgcg tcgatatcta
    32101 cccgcaactg aataaagcgt tttctggcgg taatgcggtg aacgtggcgg tgtactgcac
    32161 tcgctacggt ataaagccgg gatgcattac ctgggttggt gacgatgact acggcacaaa
    32221 gcttaagcag gatctcgcca gcatgggcgt cgatatcagc catgtccata cgaaacacgg
    32281 cgttaccgca caaactcagg tggaactgca cgacaatgat cgcgtttttg gcgactacac
    32341 cgaaggcgtg atggccgact ttgccctgag tgaagaggat tacgcctagc tggcgcagta
    32401 tgacattgtg cacgcggcaa tctggggaca tgcggaagac gcattcccac agctgcacgc
    32461 tgcgggcaaa cttaccgctt tcgacgtctc cgacaagtgg gacagcccgc tctggcagac
    32521 actggtgccg catctcgatt ttgcctttgc ctccgcaccg caagaagacg aagcgctgcg
    32581 tctgaagatg aaagcgattg ttgcccgtgg cgcaggcgtg gtgattgtca cgttgggtga
    32641 aaacggcagt attgcctggg atggcgtgca gttctggcgt caggctcctg aacaggtgac
    32701 ggttatcgac accatgggtg ccggagattc gttcattgcc ggattccttt gcggctggtc
    32761 tgcggggatg acattaccgc aggcgatggc gcagggaacg gcgtgcgcgg cacaaactat
    32821 tcagtaccac ggtgcctggt aggcataacg ttggcgtgag catcttcacg ccaacgtgct
    32881 gttacttgcc ggaaaacgac cctataatcc gtgtaattca ttctttattt cagggtcgat
    32941 tatgtcagct acggaccgct actctcatca actcctctac gctaccgtcc gccagcggct
    33001 gctggatgat atcgcgcagg gggtttacca ggccgggcaa cagatcccta ccgaaaatga
    33061 gctttgtaca caatataacg tcagccgcat taccattcgc aaagccatca gcgacttagt
    33121 ggcagacggc gtactgatcc gctggcaggg caaaggcacc tttgtacaaa gccagaaggt
    33181 cgaaaacgcc ctgctgaccg tcagtggttt taccgatttt ggcgtctcac aaggcaaggc
    33241 gacgaaagag aaagtgatcg agcaagaacg gatcagcgcc gcgccgtttt gcgaaaagct
    33301 caatatcccc ggtaacagcg aagtgttcca tctttgccgg gtgatgtatc tcgataaaga
    33361 gccgctattt attgatagtt cgtggatccc gctgtcgcgt tatcctgact ttgatgagat
    33421 ttacgtcgaa ggaagctcca cctatcagtt atttcaggag cgttttgaca cgcgagtggt
    33481 cagcgacaaa aagaccatcg atatctttgc cgccacccgc ccgcaggcaa aatggctgaa
    33541 atgcgaactg ggcgaaccgt tgtttcgcat cagcaaaatc gcctttgatc agaatgacaa
    33601 accggtgcac gtctccgaac tcttctgccg cgccaaccgc atcaccttaa caattgataa
    33661 taaaagacat taaccgtagg ccggataaga tgcaccagca tcgcatccgg cgatgccggc
    33721 gcactgaatt ttacatcccg cacgttcccc tcaccctaac cctctcccca aaggggcgag
    33781 gggaccgtcc gagcgttttg atattacggc ttcgccacaa aaccaatcgc ttcgtacacc
    33841 gcttttagcg tacgggaagc gtgcgcgctg gctttttctg cgccgtcttt catcacctgt
    33901 tgcaggaagg cttcatcgtt gcggaaacgg tggtaacgtt cctgcaattc agtcagcata
    33961 ccggaaacgg catcagccac ttcgcctttc agatgaccat acatcttgcc ttcgaactgt
    34021 ttttccagtt ccgggatgct ctggcccgtt accgcagaaa ggatatccag caggttggaa
    34081 acgcccgctt tgttctgcac atcgtagcga actaccggcg gctcgtcgga gtcggtgacc
    34141 gcgcgtttga ttttcttcac taccgatttc ggatcttcca gcaggccaat aacgttattg
    34201 cggttatcgt cagacttgga catcttcttg gtcggctcca gcagcgacat tacgcgcgcg
    34261 ccggatttcg gaataaacgg ctccggcacc ttaaagatat cgccgtacag cgcgttgaaa
    34321 cgctgggcga tatcacggct cagttccagg tgctgtttct ggtcttcacc caccggtacc
    34381 agattagttt gatacagcag gatgtccgct gccatcagca ccggatagtc aaacagacca
    34441 gcgttgatgt tctcggcata acgcgcagat ttatctttaa actgcgtcat gcggctcagt
    34501 tcgccgaagt aggtatagca gttcagtgcc cagcctaact gcgcatgttc cggtacgtgg
    34561 gactgaacaa aaatggtgct tttctcagga tcgataccac aagccagata caaggccagc
    34621 gtatccagcg tcgctttacg cagcttctgt gcatcctggc gcacggtgat cgcgtgttgg
    34681 tcaacgatac agtaaatgca atggtagtca tcctgcatgt ttacccactg acgcagcgca
    34741 cccatgtagt taccaatagt caattcacct gagggctgtg cgccactaaa aacgatgggc
    34801 ttagtcattt ttcgattcct gattttcgct atgcggaagc cctaatgcgg gcagaaggtc
    34861 atttatagac tgataaatta catcaggctg gctgagatcg atagcctcgc cgtagttata
    34921 tccgtaggtt aagccaactg atgggcaacc tgccgctttt gctgcctgaa tatcattgcg
    34981 tgagtcgcca acaaacagca tctgttgtgg agcaattccc atccgctcag ccaccagtaa
    35041 cagcgggtcc ggatgcggtt ttttgttttg cacatcatca ccaccaatca ccacgctgaa
    35101 gtatttggcg atatctaagg cttcgagcag cggcgcgacg aacggcgtcg gtttgttggt
    35161 gaccaggcct agcggcaggc ctttagcctg caacgcgccc aacgtatcgg caacgtgcgg
    35221 gaacaaaaac gtcccctctt cggcaacctc gccatagtaa cgatcgaaca gtttacgcag
    35281 aatacgtacc tgttcttctg ccggaatgtc gtcatcaacg ggcggtttac ccattgtttt
    35341 acgcagagtc gcacgttcct gacgcgccca ggtcaatgcg cgctccatca gaacatctgc
    35401 gccgttacca atccaggtaa taacgcgttc ttcacctgcg acgggcaact ccagcgcata
    35461 cagcgccata tctaccgcag cagcaagacc gggagcactg tccaccagcg taccgtcgag
    35521 atcaaaagcg acgccgcgaa tatcttcaaa cttactcatg acttaccttt gccagttcac
    35581 tgcgcatttc atcaatgact tttttgtagt ctggctggtc gaagattgcc gaaccggcaa
    35641 cgaacatatc cgcgcccgcc gcggcgattt cgccaatgtt gttcaccttc acgccaccgt
    35701 ccacttccag acgaatgtca aagccagact cgtcgatacg acgacgtact tcgcgcagtt
    35761 tatccagtgt ttgaggaatg aaagattgac cgccgaaacc agggttgacg gacatcagca
    35821 ggatcacatc cagcttatcc atcacgtaat ccagatagct cagaggtgtc gccgggttaa
    35881 ataccagacc cgctttacag ccattttctt taatcagttg cagcgtgcgg tcaacatgct
    35941 cggaggcttc tggatgaaag gtaatgatgc tggcaccagc ggcagcgaaa tcaggcacaa
    36001 tgcgatcgac cggtttcacc atcaggtgta cgtcgatagg ggcggtaatg ccgtagttac
    36061 gcaaggattt cagcaccatt ggcccaatcg tcagattggg aacgtagtgg ttatccatga
    36121 cgtcaaaatg cacgacatca gcgccagctg ccagggcttt tgcggtatct tcacccaggc
    36181 gggcaaaatc agccgacaga attgaggggg caatcaaata ctgtttcatc cgcttctcct
    36241 tgagaattat tttttcgcgg gtgaaacgac tcctggtttg tacaaagcca gcagttcgtc
    36301 caccttttta cgtgtgccgc cgttgctgct tatactgcgt cgaactttga cgacatgcaa
    36361 ttttgcgcgc tgataccact cacgcgttaa catcgtatcg tgattggaga tcagcacagg
    36421 aatatggcgc tcaaccagac cttcggcgat ctccgccaga tgcgcttgtt gttcaagcgt
    36481 aaaactgttt gtgtgatacg ccgtaaagtt ggcggtcgca gacagcggtg cataaggtgg
    36541 atcgcaatag acgacagatg catcatctgc gcgcgccatg ctatcggcgt aagactcaca
    36601 atagaaaaag gcattctgcg ctttttcagc gaagtgatac aactctgctt ccgggaaata
    36661 gggttttttg tagcggccaa acggcacatt aaactcaccg cgcagattgt aacgacacag
    36721 gccgttgtaa ccgtagcggt tcaaatataa aaacagtacc gcccgacgga acggatcctg
    36781 gcttttgttg aactcttcgc ggaactgata gtaaacctcg gcgcaatttg tttcgggaac
    36841 aaacagctcg cgtgcggcct gtacgtactc atcagtacgc atcttcacaa tgttatagag
    36901 actgatcagg tcgctattga tatcggcaag gatataacga gaaaagtcgg tgttgagaaa
    36961 caccgacccg gcacctacaa aaggctcaac cagacattcg cccttgggca aatgccgttt
    37021 aatatcatca agcaggggat acttgccccc tgcccacttc aaaaaagcgc gatttttctt
    37081 catgctgact aactaattac accttctccg gctgtggaga aagctccgac agcatccagc
    37141 gcttcaggca actcccgcaa cgataaacca ttgcgggaga taaccttgat tacttcagat
    37201 cggcctgtac ctgacgcagc ggtttcgccc acgggttttt ggcctgaaca tctgctggca
    37261 atgtagatac cgcttttttc gcctcttctt tcgaagcgta cacgccagaa accaggacat
    37321 accacggctg accattacgc gtcgtttcat agacaacgta gtttttcaga ttctctttct
    37381 tcgcccaacc gttcaggttg tcgtagttag aggaactgct cagctgcaga gtgtaatggc
    37441 tggacggtgc cgatttcaac gaacccacat tacctgcggt cttccctcca gcggctggtg
    37501 ttgccgtggt ttgcgccggg gatgccgtct gtactggagc cgtagtcgcc gtctcttttg
    37561 gtgctggcgt agaagtcgca gccggtgcct tcgtgctcgc cacaggagca gctggttcag
    37621 tacgcttcgg cgtctgtgct accggcttcg gttccgtttt cacggttgct tgcggttttt
    37681 tcggttcaat caccgcctgc tgacgagctg gacgcgtagt ggccggacgt tcagcagttt
    37741 gcgttttcgc cgtgtcacgc gatgcattgc cattgcgtac tggcgcgaca gttgctggtt
    37801 cagtcggcaa tgtagaattg accgccacat tgttcaactg ttgctgattt tgtggctggg
    37861 tcagcgcatt gttcaggtca ccctgcactt caacacgttg ttgaccatcc gttgccgccg
    37921 gggtttgccc ttgagtcggc gtagaagaga tcggtggcag agaaacatcc tgctgagtat
    37981 tttccgcaga cgtggttccc ggcgctggct gcacgccatt cgcctgatcg gtcgcattgc
    38041 cagcaagatc aatactcttc tcgccagacg cggtttgatc gctggaagtg gtcgaggggg
    38101 cttttagcgc agaaccgata ccgatgatca acagcagtag aaccagaatg ccgacgccca
    38161 tcatcatata ctgacgagaa gcgggtttac tggctgcttt tttgcgctta cgcggacgac
    38221 gctctacgcg ctcttcatcc acggtttcat cttcggattc gtcaatttct tcttcgattt
    38281 ccggttcctc attgcgctct ttttgcgcac gagtcggacg gcgatcgtca gtgtcatcaa
    38341 gttcaatatc atcaaaattg atctgcggtt cgccacgttc agtacgctca gaacgttcag
    38401 aagattgacg agaacgacca gtacgacgat cgctgggatc gggtttcagc tcgtcttctg
    38461 gtttgaattc atccatttaa caccccacta aaaggttaat gcttaccacg ttgcaattaa
    38521 cctgaagcta atagaccgct tgataagcgg cctgaccttt cttgttgtta cgctgattga
    38581 caatcggcaa tggcgttaag aacaagctcg tgcgaaacgc cgctgcgaac ttcactctta
    38641 ccaattgcca acggaagaat taagcgcatc tctcccgcaa ggactttctt gtcacgcagc
    38701 atatgcggta aatacgcctg cgcggacatt tcgcgcggcc cattgaccgg taacccagcc
    38761 cgcgtgagca gagttataat acgctgcgtt tcggcagaac taaactgccc gagacgttcc
    38821 gacgtccgcg ccgccatcac catacccgca gcgaccgctt caccatgtaa ccaattgcca
    38881 taccccattt cagcttcaat ggcatgacca aaggtgtgtc ccagattcag taaagcacgt
    38941 aacccggttt cgcgctcgtc ggcggcgaca acttctgcct tcagttcaca acaacggcga
    39001 atacagtacg ccattgccgg accgtccaga cgcaacaacg catccagatt ctcttccagc
    39061 cagttaaaaa acgtaccgtc aagaataatg ccgtatttga tgacttctgc cagccccgac
    39121 gctaactcac gcgggggaag cgttttcaga cagtcgagat ccaccaccac tgaagcaggc
    39181 tggtagaacg cgccaatcat gtttttaccg aggggatggt tgaccgcagt tttgccgcca
    39241 acggaggaat cgacctgcga cagtaacgtc gtcgggactt gaatgaaacg aacaccgcgc
    39301 tgataactcg ccgccgcgaa gccggtcaga tcgcccacta cgccgccgcc aagcgccacc
    39361 agcgtagtat cgcgaccatg cggcttttgt aacaacgccg taaagacggt atcgagtaca
    39421 gccaggcttt tatactgctc gccgtcagga aggataacgc tatcgacgtt aacacccgcc
    39481 tgttcaagta cgccgcggac cttatcgaga tacagaggag ccagggtttc gttggtgacc
    39541 aacatgacct gctcgcccga tttcagcggt aagaatgaag ctggttcatt aaacaaacca
    39601 gatgcgatgg taattgggta actacgttcc ccgagagtaa cgacaatcct ctccataacg
    39661 cgacatccac cttaattact gtacccgcag acgagtgtat ataaagccag aattagttgc
    39721 tttccagcat gtgaataatc tggtttgcaa ccactttagc gctttgatca tcagtacgaa
    39781 tggtcacgtc ggcaatctct tcatacagcg gattgcgttc attggccaac gcttccagaa
    39841 cttcacgcgg cggtgtttca acgtgcagca acgggcgttt tttatcacgc tgcgtgcgtg
    39901 caagttgctt ttcgatcgtc gtttcaagat aaacgacaac gccacgagcg gaaagacggt
    39961 tacgcgtttc acgggatttc acagagccgc cgccagtagc cagcacaata ccctgtttct
    40021 cggtcaactc attgatgacc ttttcttcgc gatcgcggaa gccttcttcg ccttctaaat
    40081 cgaaaaccca gcccacatca gctccggttc gtttctcaat ctcttgatcg gaatcgtaaa
    40141 attccatatt gagttgttga gctaactggc gcccaatagt gctttttccg gcacccatag
    40201 gcccaaccag aaagatattg cgtttctctg ccattttttc ggtactacta agactattcg
    40261 ttaatgataa acccgcttcg ctcagagagc tccgcaggac atgaactgaa acctcataag
    40321 atattgcgag agtcagactg aaaattatct caatactcca gcgggtttgg caactgaata
    40381 aatcacccat ccctctgcat atctggtcgc tgcaagcgcg ctgccttgct accaccgctc
    40441 tggcgataaa tcaccgggta agattagcgt aaaaaagaca gcaaaatgcc gcctgaatga
    40501 taaatcatca tcatggaagg caggcagcga agagcaccaa ctctcaaatc ggtactcctt
    40561 gtatgctaaa tacctgcgcg cgtcaaatag atgaaacacg ttcagcgtaa aaacattacc
    40621 gcttttacgg ctgtttactc actggaaacc agtcgtggcg tgataaacac cactaactcg
    40681 cgtcgttcat cttcttttcc gtcatgacga aataattgcc cgaaccaggg aatgtcgcca
    40741 agcaacggca cgctatcctg acccgattta tttttacggg taaaaatgcc gcccagcgcc
    40801 aacgtttctc cgcttttgac ctcgacctgc gtttcgatct cctgcttatc aatcgccagc
    40861 acttcgccat cggcctgctg tagcacctgc cccggaacgt tctggctgat gtgtaatttc
    40921 agccggatgc gacctttttg taacaccgtg ggcgtgacct ccatccccag gacggcctct
    40981 ttaaattcca ccgacgtcgc gccactttcc ccgctggaaa cctgatatgg aatttcgctc
    41041 ccctgtttaa tgctggcagg ctgaagatgt gaggccagca gacgcggact ggcgataata
    41101 tccagctgct gtttttgttc gagcgcggaa agctcaagat ccagcaaacg tccgttgatg
    41161 cgcccaatgt taaaaccgac atgcgttgtc gccgtcgcta cggagaggtc gctaccaagc
    41221 gtggtgactt gcccaacgcc accagcgtgt tgcgcatcgg ccagcgtcca tttcacgcct
    41281 aactcacgca aacttttttc attaatggtg acaatatgcg ccgacagctc aacctgcccg
    41341 accggcagat ccatttgcgc tacccactgt tcaagggcgc ttaacgccgt tttgttatct
    41401 cgtagcaaaa ggcgattggt gcgtttatcg acggtcatac tccctttggc actcagtagc
    41461 ttctccccag ctttcgccag ctctccggcg tcggcgtatt gcagagtaat attgcgattt
    41521 tccagcggca gatttgcctg cgcccgcgcc tgctccgctt cctgacgggc gatattgtca
    41581 ttctgccagg caatggaatg cactgagaga atgttgccct cctggcgcgt tatcagtccg
    41641 gcgcttttca ctacagtttg tagtgcctgc ttccagggaa catctgttag atgtaacgac
    41701 accgtaccgc tgacgtctgg cgacaccacc aggttcaact tctcctgttc agccagcgcc
    41761 tgcaacacct gagctaccgg aacgtcatcc accatcagcg tcactttttg cggctttgcc
    41821 gcctgtacgc cgggtatcag catcaacagt agtgcggcta tccattgctt catttgtatc
    41881 tccttgccgt tgccacaacc attgtggcgg ttcgcagttt gtcccggtac ccagcgttag
    41941 tacgtctggc gtcagctgta aaattgtcca gccgttttcc agcacatcgt tttgctgcac
    42001 ccgtcgccat ttcttttgcc cgtcttttat tacaccgatg atgcgctcgc ctcgccctac
    42061 catcccctga tagcgccact ggctaagttc gctaatccgg catagatctt ccggcggttt
    42121 aaaagggtca cgcataccgg ttaaaaggca caatgcaata cctgccaaca accagcgttt
    42181 aaccctcatg cggcgtctcc agttgtaggg tgaacaaaag atcatcacct tccacgctta
    42241 atgaaaaacg gctcacgctg acgttgcgct ctgccagccg tgtaaatgcc gacggcactg
    42301 cttcccacag cgttttcaac gccaactcgc ctccctgcgc ggatggatgc cagaaaacca
    42361 gttgtgcgcc gggcaactga aaatccagtg gcgaaaaggg cagcgttttt tcttcgctga
    42421 agggagtggt gtcaaccagg cgatacagtg tggcccaatg ctgatgatga cttgcccgta
    42481 gtcgaattaa tgcgtcgcgc tcttcatggt gtgtcgatga cagaaaaatg agcgtaacta
    42541 acatcagcaa ccagaatgcc cagcaaaact ggcggaggcg gggtgatgtg gcgaaccacc
    42601 agtcaaagaa catgttcatc gctaaccttc cttgttaact gatactcaaa ttgccagcgt
    42661 ccctgcgcat cctgctgcgt ggccccccgc tgattgagat gaaaagatgc atcctggcgg
    42721 agtgacgttt ctagtgcgtt taacgcggta atgcttgttg taagcccctt gatctccagc
    42781 gttccctgct gccagcttat cgttgtcagc caggcgtgct cgggtaaaag cgccgccagt
    42841 gcttccagcg cagattgcca gtcgcgggta aattgtcgct ggcgctgcct ttgcaaacgc
    42901 tgctcacgaa tttgttggcg ctccagcaaa cgtggtttgg ttatctgtaa gctgcgggcg
    42961 agttgttgtt ccccctgaag caaaacggcg tctacgcgag cttcggcgct gcttgtcaga
    43021 cgcagtatta gcgttatccc gacggccagc agcagaggcg caacgaacat cagcaaccag
    43081 aaacgaagaa aagcggtccg gcgttgctgt cgccagggca aaaaattaat tggcgggttc
    43141 atcagtacgc cttcccaagc gccagcccag cgcgatggca aagtctccac ctggcggcgg
    43201 taacggcggc tgacgaacag aaacggcctc ccaaggatca aatccgcctt cgccacatat
    43261 cgcgacgctt tcgggatcaa cggataacgc tgccgccagc tccttcgcgc tagtcatccc
    43321 caccgccagt ttgcgccccc agcgatagcg cgtcgcccac agccactgtt cgttatcacg
    43381 ccaggccaga cattgctgat gagaaggtaa aaaaggcagg aatcgctgta atgcactggc
    43441 atccggggtg atcgcactaa catgaacacg caacctttct gccaaagtaa gcagcgttgc
    43501 cagctctttg ctttgcgcgg cagtcacgtt ataggcggga ctgagtgagt cttcgctgta
    43561 atcgaagcgc agggagtccg gatccatatc cagctcgcga gccatcgtcc cggacagcca
    43621 ggccgtttgc tcccgctcac cgagggacat cgacgggcgc ggaaatgacc gctgtaatgt
    43681 gcgactggcg ggaaacgcca acatgatgtg atggcgctgc ggcagttcgc gactccacgg
    43741 taacaacgtt ttagccagcc gccgcgcatc aacaatccgc ccatctttga taatgtcgtg
    43801 cgccagcggc aaccgccacc agcgttgcaa aaagcattct tttgcgcccc gcacaatagc
    43861 aaccgctacc gcttcttgct gttgtaaatg caaaccaatt tgccagatct taaatgccat
    43921 tgtgatgatc tccttatcac ccgtcactct gacgggtata tcaatgcgtc tggcttgcct
    43981 ttatactacc gcgcgtttgt ttataaactg cccaaatgaa actaaatggg aaatttccag
    44041 tgaagttcgt aaagtatttt ttgatccttg cagtctgttg cattctgctg ggagcaggct
    44101 cgatttatgg cctataccgc tacatcgagc cacaactgcc ggatgtggcg acattaaaag
    44161 atgttcgcct gcaaattccg atgcagattt acagcgccga tggcgagctg attgctcaat
    44221 acggtgagaa acgtcgtatt ccggttacgt tggatcaaat cccgccggag atggtgaaag
    44281 cctttatcgc gacggaagac agccgcttct acgagcatca cggcgttgac ccggtgggga
    44341 tcttccgtgc ggcaagtgtg gcgctgttct ccggccacgc gtcacaaggg gcaagtacca
    44401 ttacccagca gctggcgaga aacttcttcc tcagtccaga acgcacgctg atgcgtaaga
    44461 ttaaggaagt cttcctcgcg attcgcattg aacagttgct gacgaaagac gagatcctcg
    44521 agctttatct gaacaagatt taccttggtt accgcgccta tggtgtcggt gctgcggcac
    44581 aagtctattt cggaaaaacg gtcgaccaac tgacgctgaa cgaaatggcg gtgatagccg
    44641 ggctgccgaa agcgccttcc accttcaacc cgctctactc gatggatcgt gccgtcgcgc
    44701 ggcgtaacgt cgtgctatcg cggatgctgg atgaaggata tatcacccag caacagttcg
    44761 atcagacacg cactgaggcg attaacgcta actatcacgc gccggagatt gctttttctg
    44821 cgccgtacct gagcgaaatg gtgcgccagg agatgtataa ccgttatggc gaaagtgcct
    44881 atgaagacgg ttatcgcatt tacaccacca tcacccgcaa agtgcagcag gccgcgcagc
    44941 aggcggtacg taataacgtg ctggactacg acatgcgcca cggctatcgc ggcccggcaa
    45001 atgtgctgtg gaaagtgggc gagtcggcgt gggataacaa caagattacc gatacgctga
    45061 aggcgctgcc gacctatggt ccgctgctgc ctgccgcagt caccagcgcc aatcctcagg
    45121 aagcgacggc aatgctggcg gacgggtcga ccgtcgcatt gagtatggaa ggcgttcgct
    45181 gggcgcgtcc ttaccgttcg gatactcagc agggaccgac gccgcgtaaa gtgaccgatg
    45241 ttctgcaaac gggtcagcaa atctgggttc gtcaggttgg cgatgcatgg tggctggcac
    45301 aagtgccgga agtgaactcg gcactggtgt cgatcaatcc gcaaaatggt gccgttatgg
    45361 cgctggtcgg tggctttgat ttcaatcaga gcaagtttaa ccgcgccacc caggcactgc
    45421 gtcaggtggg ttccaacatc aaaccgttcc tctacaccgc ggcgatggat aaaggtctga
    45481 cgctggcaag tatgttgaac gatgtgccaa tttctcgctg ggacgcaggt gccggttctg
    45541 actggcagcc gaaaaactca ccaccgcagt atgccggtcc aattcgctta cgtcaggggc
    45601 tgggtcagtc gaaaaacgtg gtgatggtac gcgcaatgcg ggcgatgggc gtcgactacg
    45661 ctgcagaata tctgcaacgc ttcggcttcc cggcgcaaaa cattgtccac accgaatcgc
    45721 tggcgctggg ttcagcgtcc ttcaccccaa tgcaggtggc gcgcggctac gcggtcatgg
    45781 cgaacggcgg cttcctggtg gacccgtggt ttatcagcaa aattgaaaac gatcagggcg
    45841 gcgtgatttt cgaagcgaaa ccgaaagtag cctgcccgga atgcgatatt ccggtgattt
    45901 acggtgatac gcagaaatcg aacgtgctgg aaaataacga tgttgaagat gtcgctatct
    45961 cccgcgagca gcagaatgtt tctgtaccaa tgccgcagct ggagcaggca aatcaggcgt
    46021 tagtggcgaa gactggcgcg caggagtacg caccgcacgt catcaacact ccgctggcat
    46081 tcctgattaa gagtgctttg aacaccaata tctttggtga gccaggctgg cagggtaccg
    46141 gctggcgtgc aggtcgtgat ttgcagcgtc gcgatatcgg cgggaaaacc ggtaccacta
    46201 acagttcgaa agatgcgtgg ttctcgggtt acggaccggg cgttgtgacc tcggtctgga
    46261 ttggctttga tgatcaccgt cgtaatctcg gtcatacaac ggcttccgga gcgattaaag
    46321 atcagatctc aggttacgaa ggcggtgcca agagtgccca gcctgcatgg gacgcttata
    46381 tgaaagccgt tcttgaaggt gtgccggagc agccgctgac gccgccgccg ggtattgtga
    46441 cggtgaatat cgatcgcagc accgggcagt tagctaatgg tggcaacagc cgcgaagagt
    46501 atttcatcga aggtacgcag ccgacacaac aggcggtgca cgaggtggga acgaccatta
    46561 tcgataatgg cgaggcacag gaattgttct gattaaaaag gcgcttcggc gccttttcag
    46621 tttgctgaca aagtgcactt gtttatgccg gatacgggtg aacgcgttat ccggctaaca
    46681 aaatcgtgaa aactcaatat attgcaggaa ccccataagc ctgataagca ttgtgcatca
    46741 ggcaaacttc acgcatttac actcgcccct gccctttcaa ccattcgcgc acgaggaaca
    46801 gcgcgctgac attacgcgct tcgttgaagt cagggtcttc cagcaaatcc atcatatgcg
    46861 ccagcggcca gcgcacctgt ggtagcggct ctggctcatc gccttccagc gattccgggt
    46921 agagatcttg cgctaccacg atattcattt tgctggaaaa gtaagacggt gccatgctga
    46981 gcttcttcaa aaaagtcaga tcgttcgcac caaatccaac ctcttctttt agctcgcggt
    47041 tagcggcttc gaagacgctt tcacccggat caattaatcc tttcgaaaaa cctaattcgt
    47101 aggattcagt tcccactgca tattcgcgga tcaggatcag gtgatcgtcc acaatcggca
    47161 caatcatcac tgcttcccgg ttggttggac gcattcgttc ataaacacgc cgcacgccat
    47221 tgctgaactc tagatccacg ctctcgacgg taaacagtcg ggaacgggct acagtttcaa
    47281 cattcagaat ggtgggtttt tgtaatgatt tgctcatcgt gggatctatg ctgtgaaatc
    47341 agcggttatt gtgcgatatc ggacacgctt tcggcaatgt gaattgcatg ttatttacat
    47401 ttatgtaact taataaataa tcgtcctcaa atcaaattaa aagtcaatag gttgaaataa
    47461 ctccaggaat ttgctgatat tccgccttca gagggtttgc tatgatcagc ggttactgtg
    47521 atgtgcttaa tgatgctcaa gttaaactcc acgcttgccg atagccaacc gcagaatcat
    47581 gtattgtgtc cggtgcgact gaccacgcct gacagactaa gtaagatggg gaaagcatga
    47641 gcaccattgt gattttttta gctgctttgc tggcctgctc actacttgcg ggatggctga
    47701 taaaagtgcg atccagacgg cgtcagctgc cctggaccaa cgccttcgcg gatgcgcaaa
    47761 cgcgtaaact cacacctgaa gaacgtagcg ccgttgaaaa ttatcttgag agcctgacgc
    47821 aggtattaca ggtgcctggc ccaacgggag ccagcgcggc accgatctct ctggcgctga
    47881 atgccgaaag caacaacgtc atgatgctga cacacgctat cacgcgttac ggcatctcta
    47941 ccgacgatcc gaataaatgg cgttactacc tcgattcggt agaagtccac ctgccccctt
    48001 tctgggagca gtacatcaac gatgagaata ccgttgaact gattcatacc gattcgctgc
    48061 cgctggttat ttcactcaac ggtcatacgc tgcaggagta catgcaggaa actcgcggct
    48121 atgccttgca acctgttccg tccacgcagg cgtcgattcg cggggaagag agtgagcaaa
    48181 tcgagctact caatattcgc aaagaaacgc atgaagaata tgcgctgagt cgtccgcgcg
    48241 ggctgcgtga agcgttgctg atcgtcgcct ccttcctgat gttctttttc tgcctgatta
    48301 ccccggatgt atttgttccg tggctggcag gcggcgcgtt actgctgctg ggcgcaggtc
    48361 tgtgggggct attcgccccc ccggcaaaat cctccctgcg ggaaattcat tgtctgcgcg
    48421 gtacaccccg tcgttgggga ttgtttggcg aaaacgatca ggaacagatc aacaatattt
    48481 cactcggtat tatcgacctg gtctatcccg cacactggca gccatacatt gctcaggatc
    48541 tcggtcaaca aaccgatatt gatatctatc tcgaccgcca cctagtgcgt caggggcgat
    48601 atctttcgct gcatgatgaa gtaaaaaact ttccgttaca gcactggctg cgcagtacga
    48661 ttatcgctgc gggctcgctg ctggtgctgt ttatgctgtt attctggatc ccgctggata
    48721 tgccgctgaa attcactctc tcatggatga aaggtgcgca gaccattgaa gccaccagcg
    48781 taaaacaact ggctgacgct ggcgtgcggg taggcgatac attgcgtatt agcggtacgg
    48841 gaatgtgtaa tattcgcact tccggaacct ggagcgcgaa aaccaattca ccttttttac
    48901 cgtttgactg ctcgcagatc atctggaacg acgcccgctc attaccatta ccagaatctg
    48961 aactggtcaa caaagcgacg gcattgactg aagcagttaa tcgccagctg caccctaaac
    49021 cggaagatga atctcgcgtc agcgcctcat tacgttcagc aattcaaaaa tccggcatgg
    49081 tattgcttga tgattttggc gacattgtac tgaagacagc ggatctgtgt tctgccaaag
    49141 atgactgtgt gcgactgaaa aatgcgctgg tcaatctcgg caacagtaaa gactgggacg
    49201 cgctggtaaa acgcgccaac gccgggaagc tcgatggcgt gaatgtgtta ttacgcccgg
    49261 tgagtgcgga atcgctggat aacctggtgg caacctccac cgcgccgttt atcacgcatg
    49321 aaacggcgcg agcggcacaa tcactaaaca gtccggcccc cggcggattc ctgattgtca
    49381 gcgacgaagg cagcgatttt gttgatcagc cctggccttc ggcatcactt tacgactacc
    49441 cgccgcaaga acagtggaac gctttccaga aactggcaca aatgctgatg catacgccgt
    49501 ttaacgccga gggtatcgtc acaaaaatct tcactgacgc caatggtacg cagcatatag
    49561 gccttcatcc gatcccggat cgttccggcc tgtggcgcta tctcagcacc acgctgctgc
    49621 tactgacgat gctgggtagc gccatttata atggcgtaca ggcctggcgg cgttaccagc
    49681 gtcatcgcac tcgcatgatg aagattcagg cctattatga aagctgcctg aacccgcaac
    49741 tgatcacccc ttcagaaagc cttatcgaat aacacgtttg cgcggcaggt tatgctaccc
    49801 tgtcgcgcaa attgcttcac tctggagatt ttcctcatgc atatcaacat tgcctggcag
    49861 gacgtagata ccgttctgct ggatatggac ggcacgttgc tcgacctcgc cttcgataac
    49921 tatttctggc aaaagctggt gcctgaaaca tggggcgcga aaaacggggt tacgccacag
    49981 gaagcgatgg aatatatgcg ccagcaatat cacgacgtac agcatacgct aaactggtac
    50041 tgtcttgatt actggagtga gcaactgggt ctggatatct gtgcgatgac caccgagatg
    50101 ggaccgcgtg ccgtactgcg tgaagatacc attccgtttc ttgaggcact gaaagccagc
    50161 ggtaagcagc gaattttgct caccaatgcg catccgcaca acctggcggt aaaacttgag
    50221 cataccggtc tggacgcaca ccttgattta ttactttcca cccacacatt tggttatccg
    50281 aaagaggatc agcggttatg gcatgcggtg gccgaagcta cgggtctgaa agctgaaaga
    50341 acgctgttta ttgatgacag cgaagcgatt ctcgatgctg ccgcgcaatt tggtattcgt
    50401 tactgcctcg gcgtgactaa tcctgactca gggattgccg agaaacagta tcaacgccat
    50461 ccgtcactga atgactaccg ccgcctgatc ccctcgctaa tgtgaaggag acgccatgaa
    50521 agaaaaacct gctgttgagg ttcgactgga taaatggcta tgggctgccc gtttttataa
    50581 aacccgcgcg ctggcccgtg aaatgattga aggcggtaag gtgcattaca acgggcagcg
    50641 cagcaagccg agcaaaatcg tcgagctgaa tgccacgctc actctgcgcc agggaaatga
    50701 cgaacgcacg gtgattgtaa aggcgattac tgaacagcgt cgccccgcca gcgaggcagc
    50761 cttgctgtat gaagagactg cggaaagtgt agagaaacgc gaaaaaatgg cgctggcacg
    50821 taaacttaat gccttaacca tgccgcaccc ggaccgacgc ccggacaaaa aagagcgccg
    50881 cgacctgtta cgatttaaac acggcgacag tgaataactg tcacctgcaa gagagatgat
    50941 tatgccgcaa catgaccaat tacatcgcta tctgtttgaa aactttgccg tgcgcggcga
    51001 actggtaacc gtttcggaaa ccctgcaaca gatccttgag aatcacgatt atccgcagcc
    51061 cgttaaaaac gtgctggcag aactgctggt tgcgaccagc ctgttaaccg ctacgctgaa
    51121 gtttgatggt gatatcaccg tacagctgca aggcgacggt ccgatgaatc tggcggttat
    51181 taacggtaac aacaaccagc agatgcgcgg tgtggcgcgc gtgcagggcg aaattccaga
    51241 aaatgccgac ctgaaaacgc tggtcggcaa tggttacgtg gtgatcacca ttaccccgag
    51301 cgaaggtgaa cgctatcagg gcgtggtggg tctggaaggt gataccctgg cggcctgcct
    51361 ggaagattac tttatgcgtt ctgaacagct gccgacgcgc ctgtttattc gcaccggcga
    51421 cgtagacggc aaaccggctg caggcggtat gttgttgcag gtaatgcctg cgcaaaatgc
    51481 ccagcaggac gactttgacc acctggcgac gctaaccgaa accatcaaaa ccgaagaact
    51541 gctgacctta ccggcaaacg aagtgttgtg gcgtttgtat cacgaagaag aggtgacggt
    51601 ttacgatccg caggatgtgg agttcaaatg cacctgctcg cgtgaacgtt gcgccgatgc
    51661 gctgaaaacg ctgcctgatg aagaagtcga tagcatcctg gcggaagatg gcgaaattga
    51721 catgcattgt gattactgcg gtaaccacta tctgttcaat gcgatggata ttgctgaaat
    51781 ccgcaacaac gcgtctccgg cagatccgca agttcattaa tgagtatgtc cggcagagag
    51841 agctctgccg gattttacga ctaatacacc tgatgcgctt tgtcactcat tcagattcac
    51901 atccagcctt tcaccatcca ctacaagatc gcgccacgct ttaggcttcg ttcccagttt
    51961 tccatccttc gcagctgtga gtaatttttc tttcgttata tcacgcggaa gtaaactcat
    52021 cgtaagctct tgccattctt tcttgtcagg atcaaagcca tagacaatgt atctttcatc
    52081 atcaaaggca aagaggatcc gctctgccct gccatcacta ttcagatctt gctcgaccag
    52141 tacgcaggcg tctttttcaa tacaggtcat cacgttatag cgatcctgga ttaaggccga
    52201 ccagaatgtc gcatccggtt taccagaacc aggggcaatt aacacatttt cggctaatac
    52261 tttttccgat atctgttgct gaagatgttg ctctccatct aacgccatca gtagatcccg
    52321 cgcgcgtttc gggtctttca tatactcggc atcgcttttc agcgactcaa gcgccgcacg
    52381 tccatagcga ccgctgtgtt cgagcatgta gatagttacc tggtctggcg tgtttttgcc
    52441 gctctgataa cgcgccatat gactgttcac actaatgcgc atactgtcca gcaccggcga
    52501 attaagaagc accagtatta ccaacaccaa taacgaaacc gcaaggttca ctttgccctg
    52561 aagaacaagg ggattttgcc ctttacgcca gacgatactg acaaaatacc ccagcgacca
    52621 taccagtaac accagcaccg ccagcgcgcc ctgcaagcgg tcgacagtcc agccgtactg
    52681 agcgacccgc agccataacg cccaggcggc gatgaacaca tacagcggag caaccagcag
    52741 agcggtttta atcaggcaac gcaacggccc cgtccaggga agtgacgctt tttgcggatc
    52801 gcggacaata gccattaaga tcaattgcaa aaaggccagc gttaacagca acccggcggc
    52861 ggagatgtgg cgagaaatcg cactcaggcc tgtaaacggc agggtgatga taaacatcag
    52921 ggttagcaat gatactaacg gcagcaaccc cgtggcgatt aacgtgaaca acttttgaat
    52981 agagtcgatt aaacgtgact gtgttcgcgc gaggatcacc gccagcgcgg taaccagacc
    53041 taacgtgaga taaataaacc agtcggttgc aaaaaagagc gttttgaaaa acgtgatgcc
    53101 aacaagttta aacaactcac tccacagtaa aagcaccagc cacgtcagac cattggcgag
    53161 aaaaatcacc agtaatataa gtacgttatg ccatactgac tggtaaaaat agcgatagcg
    53221 gctactgtca ttgcggatgc gaaggctttg ttgtatccac ggtagcaaca acattgccat
    53281 cagcagcaga tagcaaccaa aatcccaaag cgccttttca gctctccagg gggtcatgcc
    53341 atcagtttgc cacttcagcc agccgctcat ccccagcgtg gcaataaaca ccagcgccag
    53401 ccatccccaa aggcgcttct gtttaaaaga gatcacggag aagagtaaaa cggatgaaaa
    53461 cgcgacggtc gcaggcacgc cataaacaat ccaactgtga ttttttccgg aaagccaggc
    53521 gatcagcagg tagcaaacca gaccctgaag taatccggta gcaatcatcc cccagcgtgt
    53581 tgcgggtgaa agttcaacgt tatccatgtt ggttatccag aatcaaaagg tgggttaatt
    53641 atcgcatccg ggcagtagta ttttgctttt ttcagaaaat aatcaaaaaa agttagcgtg
    53701 gtgaatcgat actttaccgg ttgaatttgc atcaatttca ttcaggaatg cgattccact
    53761 cacaatattc ccgccatata aaccaagatt taaccttttg agaacatttt ccacacctaa
    53821 aatgctattt ctgcgataat agcaaccgtt tcgtgacagg aatcacggag ttttttgtca
    53881 aatatgaatt tctccagata cgtaaatcta tgagccttgt cgcggttaac acccccaaaa
    53941 agactttact attcaggcaa tacatattgg ctaaggagca gtgaaatgcg cgttaacaat
    54001 ggtttgaccc cgcaagaact cgaggcttat ggtatcagtg acgtacatga tatcgtttac
    54061 aacccaagct acgacctgct gtatcaggaa gagctcgatc cgagcctgac aggttatgag
    54121 cgcggggtgt taactaatct gggtgccgtt gccgtcgata ccgggatctt caccggtcgt
    54181 tcaccaaaag ataagtatat cgtccgtgac gataccactc gcgatacttt ctggtgggca
    54241 gacaaaggca aaggtaagaa cgataacaaa cctctctctc cggaaacctg gcaacatctg
    54301 aaaggcctgg tgaccaaaca gctttccggc aaacgtctgt tcgttgtcga cgccttctgt
    54361 ggtgcgaacc cggatactcg tctttccgtc cgtttcatca ccgaagtggc ctggcaggcg
    54421 cattttgtca aaaacatgtt tattcgcccg agcgatgaag agttggcagg tttcaaacca
    54481 gactttatcg ttatgaacgg cgcgaagtgc actaacccgc agtggaaaga acagggtctg
    54541 aattccgaaa acttcgtggc ttttaacctg accgagcgca tgcagctgat tggcggcacc
    54601 tggtatggcg gcgaaatgaa gaaagggatg ttctcgatga tgaactacct gctgccgctg
    54661 aaaggtatcg cttctatgca ctgctccgcc aacgttagcg agaaaggcga tgttgcggtg
    54721 ttcttcggcc tttccggcac cggtaaaacc accctttcca ccgacccgaa acgtcgcctg
    54781 attggcgatg acgaacacgg ctgggacgat gacggcgtgt ttaacttcga aggcggctgc
    54841 tacgcaaaaa ctatcaagct gtcgaaagaa gcggaacctg aaatctacaa cgctatccgt
    54901 cgcgatgcgc tgctggaaaa cgtcaccgtg cgtgaagatg gcactatcga ctttgatgat
    54961 ggttcaaaaa ccgagaacac ccgcgtttct tatccgatct atcacatcga taacatcgtt
    55021 aagccggttt caaaagcggg tcacgcaacg aaagttatct tcctgaccgc agacgccttt
    55081 ggtgtgctgc caccggtttc tcgtctgact gccgatcaaa cccagtacca cttcctctct
    55141 ggcttcaccg ccaaactggc cggtactgag cgtggcatca ccgaaccgac gccaaccttc
    55201 tccgcttgct tcggcgcggc attcctgtcg ctgcacccga ctcagtacgc agaagtgctg
    55261 gtgaaacgta tgcaggcggc gggcgcgcag gcttatctgg ttaacactgg ctggaacggc
    55321 actggcaaac gtatctcgat taaagatacc cgcgccatta tcgacgccat cctcaacggt
    55381 tcgctggata atgcagaaac cttcactctg ccgatgttta acctggcgat cccaaccgaa
    55441 ctgccgggcg tagacacgaa gattctcgat ccgcgtaaca cctacgcttc cccggaacag
    55501 tggcaggaaa aagccgaaac cctggcgaaa ctgtttatcg acaactttga taaatacacc
    55561 gacacccctg cgggtgccgc gctggtagcg gctggtccga aactgtaatg atttgaagct
    55621 ggagaatatc tatccagtat cttatagaaa gcaaaacggg aggcaccttc gcctcccgtt
    55681 tatttaccct tcttttgtcg tgctctgcgc ccgcgttacc ggcacaggca gccaggcgcg
    55741 aatggaaagc ccgccccgct cgctggtgcc aagctccagc atcccgttat ggttatccac
    55801 gatacgctgc acaatcgcca gccctaatcc cgtgccgcta atggtgcgtg cactgtcgcc
    55861 gcgaacaaac ggctggaaaa ggtgcttacg ttgttccggc gcaattcccg gaccgtcatc
    55921 ttccacctgg aaccaggcgc gattcggctc cgtcccgctg ctgactttga tccagccatt
    55981 gccgtaacgg gcggcgttga ccaccatatt cgccaccgcg cgtttgatcg acagcgggtg
    56041 cattttcact tcaatgctgc cggggtaaag cgcggtttca atttcccgct catagccact
    56101 ttcggcagca ataacctcac cgagtactgc gttgagatcc gccatttcca tcggcatctc
    56161 ctgtccggtg cgcaggtagt cgataaactg ctcaatgatg gcattgcact cctcgatatc
    56221 tttattgatc gattctgcca ggtagccatc ctgctcgctc atcatctcgg tcgccaggcg
    56281 aatacgcgtc agcggtgtac gcaagtcgtg acttaccccc gccatcagca gcgtgcggtc
    56341 atccgccagt tgcttaacac cagccgccat atggttaaag gcgcgggtaa cggaacgcac
    56401 ctcggacgcg ccatactcac gcagcggcgg cggaataatc cctttaccaa cctgcaaggc
    56461 tgcgtgttcg agatcgacca acggtcgatt ctggatacga ataaacagcc acgccccgcc
    56521 tatcgccaat agcataatcg ccaacgtata gcggaacagc ggagagaaat cgccctgatg
    56581 aatttcggtc agcggcacgc gtacccagat attgggcgac agccaggttt tcagccagac
    56641 gacaggcgaa cttttgttga cctcaacgcg cacttccgtc gggccgccca gttgctgtgc
    56701 catctgatgg cttaagaatt catagtgttg cgcccaacgc agacctgcct cttcggcagc
    56761 ctcgttggag tagagagaga tccccagctc acggtagatc tcccgacgga aagcgggagg
    56821 cacaaccaac tgcgtgccgt cctccagttg cagtttgtcg gtcatcaaca tacgcacttc
    56881 gtacgcgagg actttattaa actgctggag gctcggcaaa atcgcgaagt tcagcaccac
    56941 cagataagtc gtcaccaggc tggcgaacag caaggtgacg atgagcaata acgtacgggc
    57001 aaatgaactt cgtggcgaga agcgcaatcg cctcatgctt tagagccgtc cggtacaaag
    57061 acgtagccca ggccccaaac ggtctgaatg taacgcggat gcgctggatc ttcttccacc
    57121 atgcggcgca gacgagaaat ctgcacgtcg atggagcgtt ccattgcgga atattcacga
    57181 ccacgggcaa ggttcatcag cttatcgcgg gagagcggct cacgcggatg gctgaccagt
    57241 gccttcagta ccgcaaactc accgctggtg agcggcatcg gctcgtcttc gcggaacatt
    57301 tcgcgcgtac cgaggttaag tttgaactta ccgaaagcaa ttaccgcctc ttcctgtgac
    57361 ggtgcgcctg gtagctcgtt cgcctgacga cgcagcaccg cacggatacg ggccagcagt
    57421 tcacgcgggt taaacggttt tggaatgtaa tcgtcagcgc caatctccag gccaacgata
    57481 cggtccactt cttccccttt cgccgtcacc ataatgatcg gcatcgggtt gctctgacta
    57541 cgaagacgtc ggcaaatcga caagccatct tcaccaggta acattaaatc cagtaccata
    57601 agatggaaag attcacgagt cagcaggcga tccatctgtt ctgcattagc gacgcttcga
    57661 acctggaagc cttgttcggt gagataacgt tccagcagcg cacgcaggcg catgtcgtca
    57721 tcgaccacca gaatcttgta gttctcttgc attgtttgta ctcccaaagg ttcgcaacaa
    57781 tttgtaagcg tgtattctta aaaaagctca cgttcgtcac cagctaaatc tggtatgaat
    57841 ttcagcctaa attgttacaa agcatattaa acagcagctt aagtatacaa tttattcggc
    57901 gaaacattat tgattctgtt gatatgatca cgttataccc aatgtgcgca ttatcaaaca
    57961 gacaaaggga atcaacgaga tgaaaacgcc cctggttacc cgggaagggt atgaaaaact
    58021 caaacaagag cttaattatc tctggcgtga agaacgcccg gaggtcacaa aaaaggtgac
    58081 ctgggccgca agtctgggcg accgcagcga aaatgctgac tatcagtata ataaaaagcg
    58141 cctgcgtgaa atcgaccgtc gcgtgcgcta tctcactaaa tgcatggaaa atctcaaaat
    58201 cgtcgattac tccccgcagc aggaaggcaa agtctttttt ggcgcgtggg tggagattga
    58261 aaacgacgat ggcgtgactc accgtttccg tattgtcggc tacgatgaaa tttttggccg
    58321 taaagattac atctctatcg attccccgat ggcccgcgca ttgctgaaaa aagaagtcgg
    58381 cgatctggct gtggtgaata ctcctgccgg ggaagcgagc tggtatgtta atgctatcga
    58441 gtacgtgaaa ccgtaaggaa gagtcttaac ctcctgccga tggctggcat ttttgccagc
    58501 cagtccgtat aactatcccc tgatttttga tccgaaaaga tgaactcaaa ccatgatgaa
    58561 tgattcgttc tgccgcatta ttgcgggtga aattcaggcg cgcccggaac aggttgacgc
    58621 tgccgttcgc ctgcttgacg aagggaatac cgtgcctttt attgcacgtt atcgtaagga
    58681 aatcaccggc ggtctggatg acacgcagct gcgtaatctg gaaacgcgtc tgggatacct
    58741 gcgcgagctg gaagagagac gtcaggcgat cctcaagtcc atttccgagc aaggcaaact
    58801 caccgatgat ctggcgaatg ccatcaacgc caccctaagc aaaaccgaac tcgaagacct
    58861 ctacctgccc tacaaaccta aacgccgcac ccgcgggcaa atcgccattg aagcagggct
    58921 tgagccgttg gctgacctgc tgtggagcga tccgtcacac acgccagaag tcgccgctgc
    58981 gcaatatatt gatgccgata aaggcgtggc agataccaaa gccgcgctgg acggcgcgcg
    59041 ctatatcctg atggaacggt ttgccgaaga tgccgcgctg ctggcgaaag tgcgtgatta
    59101 tctgtggaag aacgcgcatt tggtttctac ggtggtgagc ggtaaagaag aggaaggggc
    59161 gaaattccgc gactatttcg atcatcacga accgttgtcc acggtgcctt ctcaccgcgc
    59221 gctggcgatg ttccgtgggc gtaacgaagg cgtacttcag ctttcgctga atgccgatcc
    59281 acagttcgat gagccgccca aagagagcta ttgcgagcaa atcatcatgg atcaccttgg
    59341 cctgcgcctg aacaatgccc cggcggatag ctggcgcaaa ggcgtggtga gctggacgtg
    59401 gcgcatcaag gtgctgatgc atctggaaac cgaactgatg ggcaccgtgc gcgaacgtgc
    59461 ggaaaatgaa gcaatcaacg tctttgcccg taacctgcac gatctgctga tggctgcccc
    59521 tgccggactg cgtgcaacga tgggcctcga tccgggtctg cgtaccgggg taaaagtggc
    59581 ggtggtcgat gccactggca aactggtagc gaccgatacc atttacccgc acaccggaca
    59641 agccgcaaaa gcagcgatga ctgttgctgc gctgtgtgaa aagcataacg ttgaactggt
    59701 ggcgatcggc aacggtacag cttcccgcga aaccgaacgt ttctatctcg acgtgcagaa
    59761 gcagttcccg aaagtgaccg cacagaaagt gatcgtcagc gaagctggcg cgtcggttta
    59821 ctcggcttcc gagctggcag cgcaggagtt cccggatctc gacgtttcgc tgcgtggcgc
    59881 ggtttctatc gcccgccgtt tgcaggatcc gctggcggag ctggtgaaaa tcgatccgaa
    59941 atctatcggc gtaggtcagt atcagcatga cgtcagccag acgcaactgg cccgcaaact
    60001 ggatgcagta gtagaagact gcgtaaacgc cgttggcgtc gatctcaaca ccgcttctgt
    60061 tccgctatta actcgcgtgg cgggcctgac gcgaatgatg gcgcaaaaca tcgtggcctg
    60121 gcgcgatgag aacggccagt tccagaaccg tcagcaactg ctgaaagtca gccgtctggg
    60181 gccgaaagcc ttcgagcagt gcgcgggctt cctgcgcatt aaccacggtg acaacccgct
    60241 ggacgcctct accgttcacc cggaagccta tccggtggtg gaacgcattc tggcagcaac
    60301 acagcaggca ctgaaagatc tgatgggtaa cagcagcgaa ctgcgtaacc tgaaagcgtc
    60361 tgactttact gatgaaaaat tcggtgtgcc gacagtaact gacatcatca aagagctgga
    60421 aaaaccgggt cgcgatccgc gtccggaatt taaaaccgct cagtttgccg atggcgtcga
    60481 gacaatgaac gacctgcaac cgggtatgat cctcgaaggc gctgtcacca acgtcaccaa
    60541 ctttggcgcg tttgtcgata tcggcgtgca tcaggacggc ctggttcaca tctcttcatt
    60601 gtcgaacaag tttgtggaag atccgcatac cgtggtgaaa gcgggcgaca ttgtgaaggt
    60661 gaaagtgctg gaagtggatc ttcagcgtaa acgtatcgcc ctgactatgc gtctggacga
    60721 gcagcctggc gaaaccaact ctcgtcgcgg cggcggtaac gatcgcccgc aaaacaaccg
    60781 cccggcagcc aaaccacgcg gtcgtgaagc gcagcctgcc ggtaacagcg cgatgatgga
    60841 cgcgctggcg gcagcaatgg gcaaaaaacg ttaaacgccc ggactggccc ctaaacctgc
    60901 gcgataaggc gttcacgccg catctggcaa cgaacgcctt gcctgacata aaagtgccgg
    60961 agaatatctc cggcatattt attccacagc caaactcaca atatatttcg ttaatattta
    61021 tcacttcatt aacaactgaa accttaatta aacattagcc agtcctggta attcgctatt
    61081 cgaattatat tttcgctgcg atataacctt gagccacatc aacattgagt cagattatta
    61141 ttcaaaccaa cattcgcaca cattttaagt attgctgata gaaaccattc tcattatcat
    61201 tgtactattg attatttaat ctctccttcg ttggcaaatc atctggtctc atgtcgctgt
    61261 caaacgcccc atgaggtagt tatccagtta atgagaaaca agtaggcacc tatgcaatac
    61321 actccagata ctgcgtggaa aatcactggc ttttcccgtg aaatcagccc ggcatatcgc
    61381 caaaaactgc tttctcttgg catgttacct ggctcctctt ttaatgtggt gcgcgtcgct
    61441 ccactcggcg accccattca tatcgaaacc cgtcgtgtga gcctggtatt acgcaaaaaa
    61501 gatctggcct tattagaagt ggaagcggtt tcctgttaat acggtgataa caacaatgaa
    61561 aaaattaacc ataggcttaa ttggtaatcc aaattctggc aagacaacct tatttaacca
    61621 gctcactggc gcacgtcagc gtgtaggtaa ctgggctggc gttaccgtcg aacgtaaaga
    61681 agggcaattc tccaccaccg atcatcaggt cacgctggtg gacctgcccg gcacctattc
    61741 tctgaccacc atctcatcgc agacctcgct cgatgagcaa atcgcctgtc actacatttt
    61801 gagtggcgac gccgacctgc tgattaacgt ggtggatgcg tctaaccttg agcgtaacct
    61861 gtacctgacg ctacaactgc tggaactcgg cattccctgc atcgtggcac tgaacatgct
    61921 cgacattgcc gagaagcaaa atattcgtat tgaaattgat gctctgtcgg cgcgtcttgg
    61981 ctgcccagtg atcccgctgg tttcaacccg tggtcgcggt attgaagcgc tcaagctggc
    62041 gattgatcgc tataaagcta acgagaatgt ggaactggtg cattacgcac agccgctact
    62101 caacgaagca gattcactgg caaaagtgat gccttccgac atcccgctga aacaacgtcg
    62161 ctggctgggc ctgcaaatgc tggaaggcga tatctacagc cgcgcctacg ccggtgaagc
    62221 gtcgcagcat cttgatgccg ccctcgcccg tctgcgtaat gagatggacg atccggcgct
    62281 gcacattgcc gatgcgcgtt accagtgcat tgctgccatc tgtgatgtgg taagcaacac
    62341 cctgacggca gaacccagcc gtttcaccac tgcggtagat aaaatcgtgc tcaaccgttt
    62401 cctcggtctg ccgattttcc tctttgtgat gtacctgatg ttcctgctgg ctatcaacat
    62461 cggcggggcg ttacagccgc tgtttgacgt cggctccgtg gctctatttg tgcatggtat
    62521 tcaatggatt ggctacacgc tccacttccc ggactggctg actatcttcc tcgcccaggg
    62581 cctgggtggc ggtattaaca ccgtgctgcc actggtgccg cagatcggca tgatgtacct
    62641 gttcctctcc ttccttgagg actccggcta tatggcgcgt gcggcgtttg tgatggaccg
    62701 cctgatgcag gcgctgggct tgccggggaa atcctttgtg ccgctgatcg tcggtttcgg
    62761 ttgtaacgta ccgtcggtaa tgggtgcacg tacgcttgat gcaccgcgtg aacgtctgat
    62821 gaccatcatg atggcaccgt ttatgtcctg cggcgcgcgt ctggctatct tcgcagtatt
    62881 cgcggctgcc ttcttcgggc agaacggtgc gctggcggtc ttctcgctgt atatgctcgg
    62941 tatcgtgatg gcggtgctga ctggcctgat gctcaagtac accatcatgc gcggtgaagc
    63001 gacgccgttt gtcatggagc tgccggtcta ccatgtacca cacgttaaaa gcctgattat
    63061 ccagacctgg cagcgtctga aaggcttcgt tctgcgtgct ggtaaagtga ttatcatcgt
    63121 cagcattttc ctgagcgctt tcaacagctt ctcgttgagc gggaaaatcg tcgataacat
    63181 caacgactcg gcgctggctt ccgtcagccg ggtgatcacc ccggtcttca agccgattgg
    63241 cgtgcatgaa gataactggc aggcaacagt tggcctgttt acgggtgcaa tggcgaaaga
    63301 agtggtggtg ggtacgctca acaccctcta caccgcagaa aatattcagg acgaagagtt
    63361 caatccggcg gaatttaacc tcggtgaaga gttgttcagc gcggtagatg aaacctggca
    63421 gagcctgaaa gacaccttca gccttagcgt actgatgaac cccattgaag ctagcaaagg
    63481 cgacggcgaa atgggtaccg gggcgatggg cgtgatggat cagaaattcg gtagcgcagc
    63541 tgccgcatac agctacctga ttttcgtcct gctgtatgta ccatgtatct cggtgatggg
    63601 agccatcgcc cgtgaatcaa gccgtggctg gatgggcttc tccatcctgt gggggctgaa
    63661 tatcgcttac tcactggcaa cattgttcta tcaagtcgcc agctacagtc agcacccaac
    63721 ttacagcctg gtgtgcattc tggcggttat cctgtttaac atcgtggtta tcggtctgct
    63781 gcgccgcgcg cgtagccggg tggatatcga actgctggca acccgcaagt cggtaagtag
    63841 ttgctgcgca gccagcacca ccggtgattg ccattaatgg cttcacttat tcaggtgcgt
    63901 gatttgctgg cgttacgggg ccgtatggaa gcggcccaga taagccagac attgaacact
    63961 ccacagccaa tgattaacgc catgctgcaa caactggaaa gtatgggcaa agccgtgcgg
    64021 attcaggaag aacctgacgg ctgcctctcc ggcagttgta aaagctgccc ggaaggaaaa
    64081 gcctgtctgc gcgagtggtg ggcgctgcgt taaccatact ccatcgcctg ttttgaaaag
    64141 cccggtatgc gtttgcatcc gggctttttt gcgtgcggct ttccataaaa atgcaattct
    64201 tgcagcgcga cgtaagttcc tctgaaagcg tctcgcaggg atgaaaactg gctaatgcac
    64261 aggtgtggag tggcgcgtag agtcgcggca ttcaaacaac aggtgaagga acgccatgag
    64321 caaaaagcag agttccaccc cacacgatgc gctgttcaaa ctctttttac gccaaccgga
    64381 gacggcacgc gattttcttg cgtttcattt accagcaccc attcacgcgc tctgtgatat
    64441 gaaaaccctc aagctggagt cgagcagctt tattgatgac gatctgcgtg aaagctattc
    64501 cgatgtgctg tggtcggtga aaacagaaca aggaccagga tacatctatt gtctgattga
    64561 acatcaaagc acctcaaaca aactgatcgc atttcgcatg atgcgttacg ctattgccgc
    64621 aatgcaaaat caccttgatg ccggatacaa aacgttgccg atggtggtgc cattgttgtt
    64681 ttaccacggt attgaaagcc cctatcccta ttcgctgtgt tggctggatt gtttcgccga
    64741 tcccaaactg gcaaggcagc tttatgcctc cgcatttccg ctgattgata tcaccgtcat
    64801 gcctgatgat gaaatcatgc agcaccgacg catgacgctg ctggagttaa ttcaaaaaca
    64861 tattcgtcaa cgcgacctga tggggctggt agagcaaatg gcctgcttat taagtagtgg
    64921 atacgctaat gacagacaaa tcaaagggct gtttaattac atactgcaaa ccggcgatgc
    64981 ggtacgtttt aacgatttta tcgacggcgt tgccgaacga tcaccgaaac acaaggagag
    65041 tttaatgact attgcggaaa gattgcggca ggagggggaa caatccaaag ccctgcatat
    65101 agccaaaata atgcttgaat ccggagtccc tcttgcagac atcatgcgct ttaccgggct
    65161 gtcagaagaa gagttggctg cggcgagtca gtaaagttct gtctcgccat tcaaaagtgc
    65221 ctacaccctc tgcttcaacg ccaccagcac gtgacgaaac tcgaccggat gcgaaataaa
    65281 cggcgcatga gccgctttgg cgaagatgta tgactcgctg tgtggccaga gtttatccag
    65341 catcggcacc actttgcgcg gcaccagacc atcgagatag ccatacagtc gcaaaaacgg
    65401 catgggtacg ttttgcagcg gcaagcggag atcaaccgtt ttgaggattt ccagcccacc
    65461 attgagcacg tcaacctccg gcatcggtaa cgccagaacg gttttcttca acgcccgcgc
    65521 atcctgacgc gccgtttcag tacccatggt ttgtaacgca aggaaccgtt ccaccgtacg
    65581 ctgaaaatca tcactgagtt gctgctgaaa tcccgccagc acgtccggtt ttatccccgg
    65641 ccactcgtca cgagcactaa aacaaggtga cgacgccacg gtgaccagcg cctgaacacg
    65701 ctcgggatgg gttaacgcaa tctggcttgc caccagcccg cccagactcc agcctaacca
    65761 aatggcttta tcaggtgcct gtcgcagcac ggcttcggcc atctccgcaa gtgacagcgc
    65821 accaaatccc cggctacgcc cgaagccggg caggtcaaca agatgcagcg taaaatgcga
    65881 gctaagttcc tcgtcaatgc aacgccacac ttcagcattc agtccccatc cgtgcagcag
    65941 cacaagatga acattcccct gacctttggt ctgccaccag atgttattca tccgctattg
    66001 ttctcttttg acttacaagg atgaacatat gctaacagta ccgggattat gctggctatg
    66061 ccgaatgcca ctggcattag gtcattgggg gatttgttcg gtctgctcac gcgccgcccg
    66121 tacagataaa acgttatgcc cacaatgtgg attacccgcc acacactccc atcttccctg
    66181 cggtcgctgc ctgcaaaaac cgccgccctg gcaaagactg gtcacggttg ccgactatgc
    66241 gccgccgtta agtctgctca tccaccagct taagttttcc cggcgcagcg aaatcgccag
    66301 cgccctgtca cgtctgttac tattggaagt cttacacgct cgtcgcacca ccgggttgca
    66361 attgccggat cgtatcatca gcgttccgtt atggcagcgg cgtcactggc gtcggggatt
    66421 taatcagagc gatttgctgt gtcagccgtt atcacgctgg ttgcactgcc aatgggatag
    66481 cgaagccatc acacgtacac gggccactgc gacccagcat tttcttagcg cccggctacg
    66541 caggcgcaac ctgaaaaatg cctttcgcct tgaattgcct gtgcaaggtc gccatatggt
    66601 gattgtggat gatgtcgtta ccaccggaag taccgtcgca gagattgcgc agttgctttt
    66661 acgcaatggt gcggcgactg tccaggtctg gtgcctttgt cgaaccttgt agagcctcga
    66721 tgatgggcgt attataacca actaaaatag tcaactatta ggccattact atgatccgta
    66781 tttccgatgc tgcacaagcg cactttgcca aactgctggc aaatcaggaa gaagggacac
    66841 aaatccgcgt atttgtgatt aaccctggca cgcctaacgc tgaatgtggt gtttcttatt
    66901 gtccgccgga tgccgtggaa gccaccgaca ccgccctgaa atttgacctg ctgaccgcgt
    66961 atgttgatga gttaagcgca ccatacctgg aagatgcaga gatcgatttt gttaccgacc
    67021 agttgggttc tcagctaaca ctgaaagcgc ctaacgccaa aatgcgtaaa gtggcagacg
    67081 atgcaccact gatggagcgc gtggagtata tgctgcagtc gcagatcaac ccacagcttg
    67141 ctggtcacgg tggccgcgtt tcactgatgg aaatcaccga agacggttac gccattctgc
    67201 aatttggcgg cggctgtaac ggttgttcca tggtcgatgt gacgctgaaa gaagggatcg
    67261 agaagcagct actaaacgaa ttcccggagc tgaaaggtgt gcgcgatctc accgaacacc
    67321 agcgcggcga acactcctac tactaagtta tcccctcttt tgtagattgc ccgatgcgac
    67381 gctaaagcgt catttcgggc ctacgttgaa catgtgccag acgttggcgc agcattgctt
    67441 tggcaaatcc ctcccttcgt ttccccgcga taatatgacc aacctctcat aatttaaatt
    67501 taccccgctc tggtgattct caaacgccag atgttacccg tatcattcac gtgggtacca
    67561 aacatactcc tgacatctga ctacaataat tagttttagt gggtatcagt cgtggtgccg
    67621 caatatctct gttcccgatt gggataatta gagcttgtcg tcagaaaatt gacgttaccc
    67681 ataacaaatg aaaggccagg taaatcatgc cattagtcat tgttgctatc ggtgtaatct
    67741 tgttattact cctgatgatc cgcttcaaaa tgaacggctt catcgctctc gtcctcgtgg
    67801 cgcttgcggt tggattaatg caagggatgc cgctggataa agttattggc tccatcaaag
    67861 ccggtgtcgg cgggacgctc ggtagccttg ccctgatcat gggttttggc gcaatgctgg
    67921 gcaaaatgct ggcagactgc ggtggcgcac aacgtatcgc caccacgctg attgccaaat
    67981 ttggtaaaaa acacatccag tgggcggtgg tactgaccgg ttttaccgtt ggttttgccc
    68041 tgttctatga agtgggcttt gtgctgatgc tgccgctggt gtttaccatc gcggcttctg
    68101 cgaatattcc actgctgtat gttggtgtac caatggcagc tgcactgtct gtgacccacg
    68161 gcttcctgcc accgcatccg ggtccgactg cgattgccac cattttcaat gccgatatgg
    68221 gtaaaaccct gctgtacggt actattctgg caatcccgac cgtgattctc gccggtccgg
    68281 tttacgctcg cgtgttgaaa ggtatcgata agccaattcc ggaaggtctc tacagcgcga
    68341 aaaccttcag cgaagaagag atgccgagct ttggcgtcag cgtctggacc tctctggtgc
    68401 cggtagtact gatggcgatg cgtgcgattg ccgaaatgat cctgccgaaa ggtcacgctt
    68461 tcctgccggt agcggagttc ctcggtgacc cggtaatggc tacgctgatt gccgtgctga
    68521 ttgcgatgtt cacctttggt ctgaaccgtg gtcgttcaat ggatcagatt aacgacacgc
    68581 tggtttcttc catcaaaatc attgcgatga tgctgttgat catcggtggt ggcggtgcgt
    68641 tcaagcaggt gctggtagac agcggcgtgg acaaatacat tgcttccatg atgcacgaaa
    68701 ccaacatttc tccgctgctg atggcctggt cgattgctgc cgtactgcgt atcgcgctgg
    68761 gttccgcaac cgttgcggca atcactgcgg gtggtatcgc ggcaccgctg attgcaacga
    68821 cgggtgttag cccggagctg atggttattg cggtgggttc cggtagtgtg attttctctc
    68881 atgtgaacga tccgggcttc tggctgttca aagagtactt taacctgact atcggcgaga
    68941 ccatcaaatc ctggtcgatg ctggaaacca ttatctcggt gtgcggtctg gtaggctgtc
    69001 tgctgctgaa tatggtgatt tgataacact gactgccgga tgcatcgcat ccggcatcaa
    69061 ccgcactcta cttcttcttc gctgcagctc tgcgccgtct gtccaaatcc ttcagcaact
    69121 tgttcacgcc atcatcggca aacatcgtct caagcgttgc ggaaagcttg cgtcgccagt
    69181 ttttatactg gtaactggtg ccaggaatat tcaccggttc ggccatatcc agccagtctt
    69241 ccggctgtag tcctaacaga gcactgttac tgtcggcaat gtagcgctgc aaaccacgat
    69301 taagcgtcgg cgtcatcgac atcaacgatg ccttatgccc ggcacgtttt ggcagacaac
    69361 catatttatg cagtgcatcc agcagccctt gcttcgccag ttcgcgatcc tgatacagac
    69421 cgcgcagtac cacttcatcc ggatacagcc ccagggtttt gcccagcgtt agatccccgc
    69481 tctcccagta gccgcgtagc gtaggcaggt catgtgtcgc cgcaaccgcc atcgactgct
    69541 ccggatacgc tttcggtgca cggaacgtct tctcgtggtc gttttcgaaa tagagcactt
    69601 tgtaggagta cacaccgctg ctgcgcagct taccgacaat ctctaccggt acggtaccga
    69661 gatcttcacc aatcaccata cagcgatgac gtttactttc gagtgccaga atcgagagca
    69721 gatcatccac cggatagtga acatacgcgc cctgatctgc cgtctcgcca tacggtatcc
    69781 accacaaacg cagcatcgac atcacatggt caattcgtaa tgcgccgcag ttttgcatat
    69841 tggcacgcaa cagctcgata aacggttcat aagcacgcgc ggtgatgata tgcgggtcca
    69901 ttggcggcaa tccccagttc tgccccaacg ggccgaggat atccggcggc gcgccaaccg
    69961 acgctttcag gcaatacagt tcacggtcgc accaggtttc cgccccacct tccgctacgc
    70021 caaccgccag atcacgatac aagccaatcg gcatttcata gccctggctt atctcccagc
    70081 aggcggcaaa ctggctgtaa gcctgccact gcaaccagag ataaaaatca acgtcatcgc
    70141 gatgttcttc gcagaactga cgcacttctg gtgaatccac gttctgatac atctctggcc
    70201 atgcaggcca gccccagcgc atttcgtcct ctttcacttg ctgggcatgt agcgcatcaa
    70261 aggctgcctg ccagaacagg ctgtcgcctt gatctgcaac aaactggcga aacgcggcca
    70321 tctgctcatc atcacgttgc gcgaaacctt tccacgccat tcgtaatgct gtcattttta
    70381 gggcggtaac cgtggagtaa tcgacccagt cggcatcccg cgcctgttgc agcgtctgtt
    70441 gcgtggtcgg caactgccac caggcctgag cctcttcgct aagatggaaa tcttcgacgg
    70501 cgttaacgtc gatataaatc acattcagcc agcggcgaga agacgggcta tacgggctgg
    70561 cgctctccgg atttgccgga tagagcgcat gaatcgggtt cagaccaatg aacgatccgc
    70621 cacgttttgc cacatccact agcatcgctt tgagatcgcc aaaatcccca ataccccagt
    70681 ttttttccga tcgcagcgta taaagctgga cgcaggcacc ccacagcttt tgtttattca
    70741 gcaacgcctg cggttcgtaa cagcgtttcg gggcgacaat caccctgcaa tgcgcgcgct
    70801 ggtcttcctg ggtaagcgtc agcgtgtgat aaccttccgg cagcttcgtc ggtagattga
    70861 acgctttgcc ccccgttata tggcctttgt actgcgttcc ttcttcggtg gtcagcagcc
    70921 agctatattc gccgctgccc tccaccacca tcggcatttt tttgccgctg gtataaacca
    70981 tgacattcgg gactggcgtt accgccactt tcgtggcggt acgttgatgc atcgcgtcaa
    71041 gcaaacgccg tttggtttcg gcgctaatcg actgcggttt accgtgggca ttgatgtaat
    71101 gagggctaat ccccgccgcc agcgcggcat tatccagacg tttgctttcc atcgagcttc
    71161 cttagcgttt tgcctgccag atacgagcct gataatcgcg aatagagcga tccgagctaa
    71221 acataccgca gcgggcggta ttgaggatcg ccgcgcgagt ccaggcctcc tggtcgcggt
    71281 acagcacatc cacctgcttt tgtgcctcta cataggctgc gaaatccgcc atcaccagat
    71341 acggatcgcc gccctgtttg ccgatactgt gcagcatctg gtcgaaggca tgcttatcgc
    71401 cgtcgctgta tttaccgctt tccagctctt tcaatactgc gtccagcacc ttatctttct
    71461 tccgccattt caccgggtcg tagcctttgg ccagaattgc cttcacttgt tccacggtat
    71521 gaccaaaaat aaagatattt tcttcaccga ctttctcggc gatttcaacg ttcgccccat
    71581 ccagcgtacc gacagtaagc gcaccattga gcgccagttt catattgccg gtaccggaag
    71641 cttctttacc tgcagtcgaa atttgttcgg agatatccgc cgccgggatc agtttttccg
    71701 ccgccgaaac gcaataatcc ggcaggaaca ccaccttcaa cttatcgcca accagcggat
    71761 cgttgttgat cacgtcagcc actttgttga tcgcaaagat aatatttttc gccaggtagt
    71821 agcccggtgc cgctttcgcg ccgaagagga agacgcgcgg tacgcgatca gcctgtgggt
    71881 tttcacgaat ttctttgtac aacgccagaa tatgcagcag attcaggtgc tggcgtttgt
    71941 actcatgcag acgtttgatc tgaatatcga aaatcgcctg tggattgatc tcaataccgg
    72001 tacgaacttt cacaaactcc gccagacgga ctttattcgc ctgcttgatc tcgcgatatt
    72061 gctgacggaa tttcgcatca tcagcgaatt tttccagatt gatcagctga tcgagatcgt
    72121 tagcccactc ttttttcagt gatttatcca acagagccgc cagtgccggg ttgcactgtt
    72181 tgatccagcg acgtggggta ataccgttgg tgacgttatg gaatttgttc ggccatagct
    72241 ggtgatattc cgggaacaga tctttcacca ccagatccga gtgcagcgcc gcaacaccgt
    72301 tcaccgcgaa accgccaacc acacacaggt tcgccatatg cacttgtttg tcgtgcacca
    72361 ccgccagttt agcccacact ttttcatcgc ccggccaggt tttctccacc agcgttttaa
    72421 agcgagtatt aatttcgtta ataatctgca tgtggcgcgg cagtaagcct ttcaccagtt
    72481 tcacatccca gcgttccagc gcttctggca tcagggtatg gttggtgtaa gcgaaagttt
    72541 tgctggtaat ggcccaggcg tcatcccagc tcatctggtg ctcatcgatc agcacgcgca
    72601 acagttctgg aatcgcgata gttgggtggg tatcgttcag ctgaataact tcgtaatccg
    72661 ccagttcgtg cagtttacgc cccgccagat gatggcgacg caaaatatcc gctaccgaac
    72721 aggcacactg gaagtactgc tgcatcaagc gcagcttttt accggcagta tggttgtcgt
    72781 ttggatagag aactttggtc agtttttccg cattgatgcc ctgctgttcg gcacgcagga
    72841 aatcaccgtc gttaaattta gtcagatcaa acggatgcgc gtgcgtcgcc tgccacagac
    72901 gcagcggctg cgccacgcca ttacgatagc cgacaacggg gagatcccac gcttgaccgg
    72961 taatggtaaa ctccggctcc cagcgtccgt ctttcgtcac tttaccgcca atccctacct
    73021 gcacatccag tgcttcgttg tggcggaacc acgggtagtt actgcgatgc cagtcatccg
    73081 gcgcttcaac ctgtttgcca tccacaaaag actggcggaa caaaccatat tgatagttca
    73141 gaccgtaacc cgtcgcagac tgaccgacag ttgccattga atcgaggaag cacgccgcca
    73201 gacgtcccag accaccgtta cccagcgcag ggtcgatctc ttcttccagc aggtcagtca
    73261 gattgatgtc ataagccttc aacgaatcct gtacatcctg ataccagccg agattcaaca
    73321 ggttgttgcc cgtcagacga ccaatcaaaa actccattga gatgtagtta acatgtcgct
    73381 gattcgccac cggcttggcg aatggctgag cacgcagcat ttcggccagt gcttcactca
    73441 ctgccagcca ccactggcga ggagtcattt cagccgcaga atttaagcca taacgctgcc
    73501 actgacgtga aagcgcttcc tgaaattgct tatcgttaaa aataggttgt gacataggag
    73561 ttccactttt cttagatttt caacacaacg ttatcgctag tttgccaggc tcgatgttga
    73621 ccttcctcat cctgcggggg attaggcagg gaggagttgc ggggatgagc aaggaaatgt
    73681 gatctcgacc acttaaagct agtgcaaacc acaggattag cagcaaatca atgccataca
    73741 gcgcagaaaa tctgtatcta agtgcaaaaa atggccgttg cgtattttca aaaagcgaaa
    73801 ggtaactcta taaattaagt aaaggagtga aacagtttca caagtaaaat atccagtgtg
    73861 ctccatctca ttcttaatag atttattaag atcatctttt tagatagcac tttcatcagg
    73921 aatgaagaag aaacccttgc ttaaatgaat ctgatgaaca taagggaaac cagtattcac
    73981 gctggatcag cgtcgtttta ggtgagttgt taataaagat ttggaattgt gacacagtgc
    74041 aaattcagac acataaaaaa acgtcatcgc ttgcattaga aaggtttctg gccgacctta
    74101 taaccattaa ttacgaagcg caaaaaaaat aatatttcct cattttccac agtgaagtga
    74161 ttaactatgc tgattccgtc aaaattaagt cgtccggttc gactcgacca taccgtggtt
    74221 cgtgagcgcc tgctggctaa actttccggc gcgaacaact tccggctggc gctgatcaca
    74281 agtcctgcgg gctacggtaa gaccacgctc atttcccagt gggcggcagg caaaaacgat
    74341 atcggctggt actcgctgga cgaaggtgat aaccagcaag agcgtttcgc cagctatctc
    74401 attgccgccg tgcaacaggc aaccaacggt cactgtgcga tatgtgagac gatggcgcaa
    74461 aaacggcaat atgccagcct gacgtcactc ttcgcccagc ttttcattga gctggcggaa
    74521 tggcatagcc cactttatct ggtcatcgat gactatcatc tgatcactaa tccagtgatc
    74581 cacgagtcaa tgcgcttctt tattcgccat caaccagaaa atctcaccct ggtggtgttg
    74641 tcacgtaacc ttccgcaact gggcattgcc aatctgcgtg ttcgtgatca actgctggaa
    74701 attggcagtc agcaactggc atttacccat caggaagcga agcagttttt tgattgccgt
    74761 ctgtcatcgc cgattgaagc cgcagaaagc agtcggattt gcgatgacgt ttctggttgg
    74821 gcgacggcac tacagctaat cgccctctcc gcccggcaga atacccactc agcccataag
    74881 tcggcacgcc gcctggcggg aatcaatgcc agccatcttt cggattatct ggtcgatgag
    74941 gttttggata acgtcgatct cgcaacgcgc cattttctgt tgaaaagcgc cattttgcgc
    75001 tcaatgaacg atgcactcat cacccgtgtg accggcgaag aaaacgggca aatgcgcctc
    75061 gaagagattg agcgtcaggg gctgttttta cagcggatgg atgataccgg cgagtggttc
    75121 tgctatcacc cgctgtttgg taacttcctg cgccagcgct gccagtggga actggcggcg
    75181 gagctgccgg aaatccaccg tgccgccgca gaaagctgga tggcccaggg attccccagc
    75241 gaagcgattc atcatgcgct ggcggcaggc gatgcgctga tgctgcgcga tattctgctt
    75301 aatcacgcct ggagtttgtt caaccatagc gaactgtcgc tgctggaaga gtcgcttaag
    75361 gccctgccgt gggacagttt gctggaaaat ccgcagttgg tgttattgca ggcgtggctg
    75421 atgcaaagcc aacatcgcta cggcgaagtt aacaccctgc tggcccgtgc tgaacatgaa
    75481 atcaaggaca tcagagaagg caccatgcac gcagaattta acgctctgcg cgcccaggtg
    75541 gcgattaacg atggtaatcc ggatgaagcg gaacggctgg caaaactggc actggaagag
    75601 ctaccgccgg gctggttcta tagccgcatt gtggcaacct cggtgctggg tgaagtgctg
    75661 cactgcaaag gcgaattgac ccgctcactg gcgctaatgc agcaaaccga acagatggca
    75721 cgccagcacg acgtctggca ctacgcctta tggagtttaa ttcagcaaag tgaaattctg
    75781 tttgcccaag ggttcctgca aaccgcgtgg gaaacgcagg aaaaagcgtt ccagctgatc
    75841 aacgagcagc atctggaaca gttgccgatg catgagtttc tggtgcgcat ccgagcacaa
    75901 ctgttatggg cctgggcacg gctggatgaa gccgaagcgt cggcgcgtag cgggattgaa
    75961 gtcttatcgt cttatcagcc acagcaacag cttcagtgcc tggcaatgtt gattcaatgc
    76021 tcgctggccc gtggtgattt agataacgcc cgtagccagc tgaaccgtct ggaaaacctg
    76081 ctggggaatg gcaaatatca cagcgactgg atctctaacg ccaacaaagt ccgggtgatt
    76141 tactggcaaa tgaccggcga taaagccgcc gctgccaact ggttgcgtca tacggctaaa
    76201 ccggagtttg cgaacaacca cttcctgcaa ggtcaatggc gcaacattgc ccgcgcacaa
    76261 atcttgctgg gcgagtttga acctgcggaa attgtcctcg aagaactcaa tgaaaatgcc
    76321 cgcagtctgc ggttgatgag cgatctcaac cgtaacctgt tgctgctaaa tcaactgtac
    76381 tggcaggccg gacgtaaaag tgacgcccag cgcgtgttgc tggacgcatt aaaactggcg
    76441 aatcgcaccg gatttatcag tcattttgtc atcgaaggcg aagcgatggc gcaacaactg
    76501 cgccagctga ttcaactcaa tacgctgccg gaactggaac agcatcgcgc acagcgtatt
    76561 ctacgagaaa tcaatcaaca tcatcggcat aaattcgccc atttcgatga gaatttcgtt
    76621 gaacgtctgc taaatcatcc ggaagtgcca gaactgatcc gcaccagccc gttgacccaa
    76681 cgtgaatggc aggtcctggg gctgatttac tccggttaca gcaacgaaca aatagcgggt
    76741 gaactggaag tggcggcaac caccatcaag acgcatatcc gcaatctgta ccaaaaactc
    76801 ggcgtcgccc accgtcaggc tgccgtgcag cacgcgcaga aactgctgaa gatgatgggt
    76861 tatggggtgt gagtttgtat agcgttttta tgtacactct ttatactgta tataaaatcg
    76921 cactacaggc tcatgaatat gaaaccggaa agcaaagaag cgccaatcaa tatcagagcg
    76981 aaagcctcac agcgtgacct gattgatatg gcagcgaatc tggtcgcaaa atcgcgcact
    77041 gattttatgc tggatgccgc atgccgtgaa gcgcaagaca tcttattaga tcaacgacta
    77101 ttcatccttg atgacgagca atacgatgcg tttctcgctg ccctggacgc ccccatcact
    77161 gctgagcgtc aggccaaaat taacgcgttg atgaatcgta aatcgccatg ggaataacgg
    77221 ctcctactcc cctaacgtct gaacataatc tggctgactt ttgctgttcc gatcacggga
    77281 tgaatgaatg gctaaaaaag aaagcgttaa aaaatcacag ctccggtctg tctcgtgttt
    77341 acgttatctg tattgccaat acccgccagg taatcggcta ttactgcctt tctacaggca
    77401 gtattcaacg taatttggcc cctggagcca tgcgacgtaa tgctcccgaa tcgttgcctg
    77461 ttgtcgtatt aggcagactg gcaattgatc aagcctgggc aggtaaaggg ttgggcgtcg
    77521 cattgttgaa agatgcagtc tatcgcacaa tgtccatagc tcaacaggta ggggttcgtg
    77581 cgctcattgt tcatgcctta gacgattcag tacgcaattt ctatctgaag tatgcctttg
    77641 taccttcacc attccagtca cttaccttac tgtatcccat tactctggag taatttacct
    77701 ctaccggata gtcacactga tgagtctgat ccgggaggga tttcaatcag tgagtttggt
    77761 tatcatcacc cgcgttacgc catctgtttc agccagagta aaccgcaccg gcaagaaacg
    77821 ctccaccacg gcgatattgg tcagcagatg gcatgaggga tgggcgaccg taaattgtcc
    77881 cgcgcccgcc agcgccattg gcaacactag ctggtcagca agatattccc ccactgccgc
    77941 cgggcttgcc aggtagcgtt tcacctcttt caccaactgt gccgcaacca cctcggcact
    78001 gacgcgcttt tcaccgacga caaaaaagcg ttcggtgata ttttcacttt cgacttcaag
    78061 cgagacggta ttacccggcc cctggtcgcg cggcaggtta tgaatattct gctcatgcag
    78121 ggaaaaactt gccgccagcg tagcgatttc acgctcagcg acatgccgcg gcacgccagc
    78181 taataacacc tctccacgca tctgcacaat gttcccgcgc tcgccaagtt gcaaggtgtt
    78241 aaacgatgcc accggcgaga cttccgttgc caccacgccg cctccggcag gataaaaacc
    78301 gtgacgtaac agcgtggttt gctgatgaat tcctattttc gccagcagcg gctccaacac
    78361 ccggcggata aaatccgcag gcggggccga cgggttatcg gtaccgccgc tcacttcaac
    78421 acgcgaaggt ccatcggcaa accacagcgc gggcagcacc gtttgcagca ccagcgtaca
    78481 acttcctgcg ctaccgatag caaagcgata atcgccgcca cgcacggtgc cgggccggaa
    78541 gaccagacgc tgcgacccca gctccgcgcc ttccaccgtc gccccgcaaa tttccgtcgc
    78601 cgcctttacc gccgtcagat gctggcgcaa cagtcccggt tttgcccgcc cggcacgaat
    78661 gccggtgatg gtaaatggct ggccggttat catcgacaga ctcagcgccg agcgcatgat
    78721 ctgcccgccg ccttcgccct gtgcgccatc cagcgcaatc atccttttca tcatttatcc
    78781 ttttacgcac accacctgac gtagggtgta gataacttcc accagatcgc tttgtgccgc
    78841 catcaccgca tcaatatctt tatacgccat cgggatttcg tcgatcactt cggcatcttt
    78901 acggcattcc acatgcacgg tggcacgaat ttgatcttcc acgctgaaca tttttttcgc
    78961 tttagtacgg ctcataaccc gcccggcacc gtggctgcac gaacagaacg actcttcatt
    79021 tcccagccca cggacgataa agcttttcgc tcccatcgac cctggaataa ttccatattg
    79081 accagcacgc gcagacaccg cgcctttacg cgtcacgtag atctcttcac caaagtgctg
    79141 ttctttttgc acatagttgt ggtgacagtt gatctcttcc atcgccagcg tttgtggctg
    79201 tctgaccgtt ttctgcgtaa tgctctgcaa cgccgttact acgttttcca tcatcacatc
    79261 gcggttaagg ctggcaaaaa gctgcgccca ggccacggct ttcaggtaat catcaaagta
    79321 ttccgtacct tccataaagt acgccagatc acgcgacggc aatgtttcaa gcgtttcctg
    79381 catctctttt tgcgccagat cgataaagta agtcccgatg gcattaccaa ttccgcgtga
    79441 accggagtgc agcataatcc acacctggtc cgactcatca aggcagattt caataaagtg
    79501 gttacccgtt cccagcgttc ccaggtgttt atagttgtta gtgttaagga aacgcggata
    79561 tttttgcgtt aaccactgat aaccggcctc cagctcagcc catttcgcat cgacgttaac
    79621 aggcgggttt tcccatgcgc ctttatcacg tttacaacgg ccagtggtac gcccgtgcgg
    79681 caccgcagtt tcaatcgcct gacgcagctc cgccaggttt tccggcaggt cagccgccgt
    79741 taaagcggta cgtagtgcgt tcattccaca gccaatatcc acgcccaccg ccgccggaat
    79801 aatcgcccct ttggtcggga tcacgctacc aatggtggaa cctttaccca ggtgtacatc
    79861 aggcattacc gcaatatgtt tgaaaataaa cggcatcttc gccgtattaa taagttgctg
    79921 acgcgcatcg gcttctaccg gcacgccttt ggtccacatt tttaccgggg cattttcagt
    79981 ggtcagtaat tcgtaattca ttttgttttt ctcttttcgt tgtttgcggt cctgataatt
    80041 gcaaccgtcg tgcc
//
LOCUS       DAZKIP010000010        79740 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZKIP010000010 DAZKIP010000000
VERSION     DAZKIP010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 79740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.010"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            111..695
                     /gene="yraH"
                     /locus_tag="ACHOUK_000787"
     CDS             111..695
                     /gene="yraH"
                     /locus_tag="ACHOUK_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9896296.1"
                     /translation="MNKVTKTAIAGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSAGI
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            775..1470
                     /gene="yraI"
                     /locus_tag="ACHOUK_000788"
     CDS             775..1470
                     /gene="yraI"
                     /locus_tag="ACHOUK_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP9896297.1"
                     /translation="MSKRTFAMIFTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLIRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVSDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKADLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1499..4015
                     /gene="yraJ"
                     /locus_tag="ACHOUK_000789"
     CDS             1499..4015
                     /gene="yraJ"
                     /locus_tag="ACHOUK_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP9896298.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYNFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSVNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            4026..4759
                     /locus_tag="ACHOUK_000790"
                     /pseudo
     CDS             4026..4759
                     /locus_tag="ACHOUK_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(4802..5662)
                     /gene="rsmI"
                     /locus_tag="ACHOUK_000791"
     CDS             complement(4802..5662)
                     /gene="rsmI"
                     /locus_tag="ACHOUK_000791"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFP9896299.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            5727..7763
                     /gene="lpoA"
                     /locus_tag="ACHOUK_000792"
     CDS             5727..7763
                     /gene="lpoA"
                     /locus_tag="ACHOUK_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFP9896300.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFVGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            7721..8116
                     /gene="yraN"
                     /locus_tag="ACHOUK_000793"
     CDS             7721..8116
                     /gene="yraN"
                     /locus_tag="ACHOUK_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFP9896301.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDTFNDHS"
     gene            8136..8726
                     /gene="diaA"
                     /locus_tag="ACHOUK_000794"
     CDS             8136..8726
                     /gene="diaA"
                     /locus_tag="ACHOUK_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFP9896302.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            8736..9311
                     /gene="dolP"
                     /locus_tag="ACHOUK_000795"
     CDS             8736..9311
                     /gene="dolP"
                     /locus_tag="ACHOUK_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFP9896303.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(9425..10465)
                     /gene="yraQ"
                     /locus_tag="ACHOUK_000796"
     CDS             complement(9425..10465)
                     /gene="yraQ"
                     /locus_tag="ACHOUK_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFP9896304.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(10538..11173)
                     /gene="yraR"
                     /locus_tag="ACHOUK_000797"
     CDS             complement(10538..11173)
                     /gene="yraR"
                     /locus_tag="ACHOUK_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFP9896305.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            11301..11819
                     /gene="yhbO"
                     /locus_tag="ACHOUK_000798"
     CDS             11301..11819
                     /gene="yhbO"
                     /locus_tag="ACHOUK_000798"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFP9896306.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(11799..12242)
                     /gene="yhbP"
                     /locus_tag="ACHOUK_000799"
     CDS             complement(11799..12242)
                     /gene="yhbP"
                     /locus_tag="ACHOUK_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFP9896307.1"
                     /translation="METLTAISRWLAKQHVVTWCVQQEGELWCANAFYLFDVQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRPDEIKFTDNTLGFGKKMIWLRGSGTEQA"
     gene            12293..12595
                     /gene="yhbQ"
                     /locus_tag="ACHOUK_000800"
     CDS             12293..12595
                     /gene="yhbQ"
                     /locus_tag="ACHOUK_000800"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFP9896308.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAKLLSSLQTPEIKSD"
     gene            complement(12582..13085)
                     /gene="yhbS"
                     /locus_tag="ACHOUK_000801"
     CDS             complement(12582..13085)
                     /gene="yhbS"
                     /locus_tag="ACHOUK_000801"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9896309.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(13079..13603)
                     /gene="ubiT"
                     /locus_tag="ACHOUK_000802"
     CDS             complement(13079..13603)
                     /gene="ubiT"
                     /locus_tag="ACHOUK_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFP9896310.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            13812..14807
                     /gene="ubiU"
                     /locus_tag="ACHOUK_000803"
     CDS             13812..14807
                     /gene="ubiU"
                     /locus_tag="ACHOUK_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFP9896311.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            14816..15694
                     /gene="ubiV"
                     /locus_tag="ACHOUK_000804"
     CDS             14816..15694
                     /gene="ubiV"
                     /locus_tag="ACHOUK_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFP9896312.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            15775..16782
                     /gene="yhbW"
                     /locus_tag="ACHOUK_000805"
     CDS             15775..16782
                     /gene="yhbW"
                     /locus_tag="ACHOUK_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFP9896313.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSARDAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVAWFDARDPNPHVRPVPG
                     YGEQIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(16899..18143)
                     /gene="mtr"
                     /locus_tag="ACHOUK_000806"
     CDS             complement(16899..18143)
                     /gene="mtr"
                     /locus_tag="ACHOUK_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFP9896314.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIDIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(18297..20186)
                     /gene="deaD"
                     /locus_tag="ACHOUK_000807"
     CDS             complement(18297..20186)
                     /gene="deaD"
                     /locus_tag="ACHOUK_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFP9896315.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFSGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(20179..20259)
                     /gene="yrbN"
                     /locus_tag="ACHOUK_000808"
     CDS             complement(20179..20259)
                     /gene="yrbN"
                     /locus_tag="ACHOUK_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFP9896316.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(20366..21250)
                     /gene="nlpI"
                     /locus_tag="ACHOUK_000809"
     CDS             complement(20366..21250)
                     /gene="nlpI"
                     /locus_tag="ACHOUK_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFP9896317.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(21359..23494)
                     /gene="pnp"
                     /locus_tag="ACHOUK_000810"
     CDS             complement(21359..23494)
                     /gene="pnp"
                     /locus_tag="ACHOUK_000810"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFP9896318.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(23741..24010)
                     /gene="rpsO"
                     /locus_tag="ACHOUK_000811"
     CDS             complement(23741..24010)
                     /gene="rpsO"
                     /locus_tag="ACHOUK_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFP9896319.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTRLIERLGLRR"
     gene            complement(24159..25103)
                     /gene="truB"
                     /locus_tag="ACHOUK_000812"
     CDS             complement(24159..25103)
                     /gene="truB"
                     /locus_tag="ACHOUK_000812"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFP9896320.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(25103..25504)
                     /gene="rbfA"
                     /locus_tag="ACHOUK_000813"
     CDS             complement(25103..25504)
                     /gene="rbfA"
                     /locus_tag="ACHOUK_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFP9896321.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(25669..28341)
                     /gene="infB"
                     /locus_tag="ACHOUK_000814"
     CDS             complement(25669..28341)
                     /gene="infB"
                     /locus_tag="ACHOUK_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFP9896322.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(28366..29853)
                     /gene="nusA"
                     /locus_tag="ACHOUK_000815"
     CDS             complement(28366..29853)
                     /gene="nusA"
                     /locus_tag="ACHOUK_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFP9896323.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(29881..30333)
                     /gene="rimP"
                     /locus_tag="ACHOUK_000816"
     CDS             complement(29881..30333)
                     /gene="rimP"
                     /locus_tag="ACHOUK_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFP9896324.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(30540..30616)
                     /locus_tag="ACHOUK_000817"
     tRNA            complement(30540..30616)
                     /locus_tag="ACHOUK_000817"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30580..30582),aa:Met,seq:cat)
     gene            30964..32307
                     /gene="argG"
                     /locus_tag="ACHOUK_000818"
     CDS             30964..32307
                     /gene="argG"
                     /locus_tag="ACHOUK_000818"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFP9896325.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQMENLENKGQ"
     gene            complement(32315..33940)
                     /gene="yhbX"
                     /locus_tag="ACHOUK_000819"
     CDS             complement(32315..33940)
                     /gene="yhbX"
                     /locus_tag="ACHOUK_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFP9896326.1"
                     /translation="MTVFNKFARSFKSHWLLYLSVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVAKMLGMYSPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKNAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIVHYKGDS
                     LVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(34399..34485)
                     /locus_tag="ACHOUK_000820"
     tRNA            complement(34399..34485)
                     /locus_tag="ACHOUK_000820"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34449..34451),aa:Leu,seq:gag)
     gene            complement(34500..34832)
                     /gene="secG"
                     /locus_tag="ACHOUK_000821"
     CDS             complement(34500..34832)
                     /gene="secG"
                     /locus_tag="ACHOUK_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFP9896327.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(35060..36397)
                     /gene="glmM"
                     /locus_tag="ACHOUK_000822"
     CDS             complement(35060..36397)
                     /gene="glmM"
                     /locus_tag="ACHOUK_000822"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFP9896328.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVETALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(36390..37238)
                     /gene="folP"
                     /locus_tag="ACHOUK_000823"
     CDS             complement(36390..37238)
                     /gene="folP"
                     /locus_tag="ACHOUK_000823"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFP9896329.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(37328..39262)
                     /gene="ftsH"
                     /locus_tag="ACHOUK_000824"
     CDS             complement(37328..39262)
                     /gene="ftsH"
                     /locus_tag="ACHOUK_000824"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFP9896330.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNAGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(39362..39991)
                     /gene="rlmE"
                     /locus_tag="ACHOUK_000825"
     CDS             complement(39362..39991)
                     /gene="rlmE"
                     /locus_tag="ACHOUK_000825"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFP9896331.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            40117..40410
                     /gene="yhbY"
                     /locus_tag="ACHOUK_000826"
     CDS             40117..40410
                     /gene="yhbY"
                     /locus_tag="ACHOUK_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFP9896332.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(40566..41042)
                     /gene="greA"
                     /locus_tag="ACHOUK_000827"
     CDS             complement(40566..41042)
                     /gene="greA"
                     /locus_tag="ACHOUK_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFP9896333.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            41290..42723
                     /gene="dacB"
                     /locus_tag="ACHOUK_000828"
     CDS             41290..42723
                     /gene="dacB"
                     /locus_tag="ACHOUK_000828"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFP9896334.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAVAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(42763..43935)
                     /gene="cgtA"
                     /locus_tag="ACHOUK_000829"
     CDS             complement(42763..43935)
                     /gene="cgtA"
                     /locus_tag="ACHOUK_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFP9896335.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKVEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(43951..44916)
                     /gene="yhbE"
                     /locus_tag="ACHOUK_000830"
     CDS             complement(43951..44916)
                     /gene="yhbE"
                     /locus_tag="ACHOUK_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFP9896336.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(45043..45300)
                     /gene="rpmA"
                     /locus_tag="ACHOUK_000831"
     CDS             complement(45043..45300)
                     /gene="rpmA"
                     /locus_tag="ACHOUK_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFP9896337.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(45321..45632)
                     /gene="rplU"
                     /locus_tag="ACHOUK_000832"
     CDS             complement(45321..45632)
                     /gene="rplU"
                     /locus_tag="ACHOUK_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFP9896338.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            45891..46862
                     /gene="ispB"
                     /locus_tag="ACHOUK_000833"
     CDS             45891..46862
                     /gene="ispB"
                     /locus_tag="ACHOUK_000833"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFP9896339.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLKPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            47091..47369
                     /gene="sfsB"
                     /locus_tag="ACHOUK_000834"
     CDS             47091..47369
                     /gene="sfsB"
                     /locus_tag="ACHOUK_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFP9896340.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(47417..48676)
                     /gene="murA"
                     /locus_tag="ACHOUK_000835"
     CDS             complement(47417..48676)
                     /gene="murA"
                     /locus_tag="ACHOUK_000835"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFP9896341.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNTQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(48731..48985)
                     /gene="ibaG"
                     /locus_tag="ACHOUK_000836"
     CDS             complement(48731..48985)
                     /gene="ibaG"
                     /locus_tag="ACHOUK_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFP9896342.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(49145..49438)
                     /gene="mlaB"
                     /locus_tag="ACHOUK_000837"
     CDS             complement(49145..49438)
                     /gene="mlaB"
                     /locus_tag="ACHOUK_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFP9896343.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(49438..50073)
                     /gene="mlaC"
                     /locus_tag="ACHOUK_000838"
     CDS             complement(49438..50073)
                     /gene="mlaC"
                     /locus_tag="ACHOUK_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFP9896344.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQRDAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(50092..50643)
                     /gene="mlaD"
                     /locus_tag="ACHOUK_000839"
     CDS             complement(50092..50643)
                     /gene="mlaD"
                     /locus_tag="ACHOUK_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFP9896345.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(50648..51430)
                     /gene="mlaE"
                     /locus_tag="ACHOUK_000840"
     CDS             complement(50648..51430)
                     /gene="mlaE"
                     /locus_tag="ACHOUK_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFP9896346.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(51438..52247)
                     /gene="mlaF"
                     /locus_tag="ACHOUK_000841"
     CDS             complement(51438..52247)
                     /gene="mlaF"
                     /locus_tag="ACHOUK_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFP9896347.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            52457..53434
                     /gene="yrbG"
                     /locus_tag="ACHOUK_000842"
     CDS             52457..53434
                     /gene="yrbG"
                     /locus_tag="ACHOUK_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFP9896348.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            53448..54434
                     /gene="kdsD"
                     /locus_tag="ACHOUK_000843"
     CDS             53448..54434
                     /gene="kdsD"
                     /locus_tag="ACHOUK_000843"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFP9896349.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHIPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            54455..55021
                     /gene="kdsC"
                     /locus_tag="ACHOUK_000844"
     CDS             54455..55021
                     /gene="kdsC"
                     /locus_tag="ACHOUK_000844"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFP9896350.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            55018..55593
                     /gene="lptC"
                     /locus_tag="ACHOUK_000845"
     CDS             55018..55593
                     /gene="lptC"
                     /locus_tag="ACHOUK_000845"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFP9896351.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            55562..56119
                     /gene="lptA"
                     /locus_tag="ACHOUK_000846"
     CDS             55562..56119
                     /gene="lptA"
                     /locus_tag="ACHOUK_000846"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFP9896352.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            56126..56851
                     /gene="lptB"
                     /locus_tag="ACHOUK_000847"
     CDS             56126..56851
                     /gene="lptB"
                     /locus_tag="ACHOUK_000847"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFP9896353.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            56899..58332
                     /gene="rpoN"
                     /locus_tag="ACHOUK_000848"
     CDS             56899..58332
                     /gene="rpoN"
                     /locus_tag="ACHOUK_000848"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFP9896354.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            58355..58642
                     /gene="hpf"
                     /locus_tag="ACHOUK_000849"
     CDS             58355..58642
                     /gene="hpf"
                     /locus_tag="ACHOUK_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFP9896355.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            58760..59251
                     /gene="ptsN"
                     /locus_tag="ACHOUK_000850"
     CDS             58760..59251
                     /gene="ptsN"
                     /locus_tag="ACHOUK_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFP9896356.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTKGTP
                     DEA"
     gene            59297..60151
                     /gene="rapZ"
                     /locus_tag="ACHOUK_000851"
     CDS             59297..60151
                     /gene="rapZ"
                     /locus_tag="ACHOUK_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFP9896357.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            60148..60420
                     /gene="npr"
                     /locus_tag="ACHOUK_000852"
     CDS             60148..60420
                     /gene="npr"
                     /locus_tag="ACHOUK_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFP9896358.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            60634..61266
                     /gene="yrbL"
                     /locus_tag="ACHOUK_000853"
     CDS             60634..61266
                     /gene="yrbL"
                     /locus_tag="ACHOUK_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFP9896359.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDVAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALASREA"
     gene            complement(61263..61991)
                     /gene="mtgA"
                     /locus_tag="ACHOUK_000854"
     CDS             complement(61263..61991)
                     /gene="mtgA"
                     /locus_tag="ACHOUK_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFP9896360.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDRVSMDQISPWMGLAVIAAEDQTFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(61988..62641)
                     /gene="elbB"
                     /locus_tag="ACHOUK_000855"
     CDS             complement(61988..62641)
                     /gene="elbB"
                     /locus_tag="ACHOUK_000855"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFP9896361.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGRECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(62871..65207)
                     /gene="arcB"
                     /locus_tag="ACHOUK_000856"
     CDS             complement(62871..65207)
                     /gene="arcB"
                     /locus_tag="ACHOUK_000856"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFP9896362.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVNVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(65303..66232)
                     /gene="yhcC"
                     /locus_tag="ACHOUK_000857"
     CDS             complement(65303..66232)
                     /gene="yhcC"
                     /locus_tag="ACHOUK_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFP9896363.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            66906..71366
                     /gene="gltB"
                     /locus_tag="ACHOUK_000858"
     CDS             66906..71366
                     /gene="gltB"
                     /locus_tag="ACHOUK_000858"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFP9896364.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNEI
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHNDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTET
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            71379..72797
                     /gene="gltD"
                     /locus_tag="ACHOUK_000859"
     CDS             71379..72797
                     /gene="gltD"
                     /locus_tag="ACHOUK_000859"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFP9896365.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVQAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TREEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHSMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            72981..74028
                     /gene="yhcG"
                     /locus_tag="ACHOUK_000860"
                     /pseudo
     CDS             72981..74028
                     /gene="yhcG"
                     /locus_tag="ACHOUK_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhcG family protein"
     gene            complement(74088..74552)
                     /gene="nanQ"
                     /locus_tag="ACHOUK_000861"
     CDS             complement(74088..74552)
                     /gene="nanQ"
                     /locus_tag="ACHOUK_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFP9896366.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLKGEERILFGTAGTARQCEEFHHEDDYQ
                     LCSAIENEQTINLKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(74549..75424)
                     /gene="nanK"
                     /locus_tag="ACHOUK_000862"
     CDS             complement(74549..75424)
                     /gene="nanK"
                     /locus_tag="ACHOUK_000862"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFP9896367.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLGQLTDLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADAKTIFMRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(75421..76110)
                     /gene="nanE"
                     /locus_tag="ACHOUK_000863"
     CDS             complement(75421..76110)
                     /gene="nanE"
                     /locus_tag="ACHOUK_000863"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFP9896368.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLTLVKRLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(76158..77648)
                     /gene="nanT"
                     /locus_tag="ACHOUK_000864"
     CDS             complement(76158..77648)
                     /gene="nanT"
                     /locus_tag="ACHOUK_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFP9896369.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCTAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(77757..78650)
                     /gene="nanA"
                     /locus_tag="ACHOUK_000865"
     CDS             complement(77757..78650)
                     /gene="nanA"
                     /locus_tag="ACHOUK_000865"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFP9896370.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(78772..79563)
                     /gene="nanR"
                     /locus_tag="ACHOUK_000866"
     CDS             complement(78772..79563)
                     /gene="nanR"
                     /locus_tag="ACHOUK_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFP9896371.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT        19667 a        21543 c        19776 g        18754 t
ORIGIN      
        1 agtctattta atgttcaatg aaatatttct acctgtataa tctttaaaga tgttgaacat
       61 atattcacat taaatatgat tatgtacttg ttacaaggat aaggttataa atgaataaag
      121 ttacaaaaac agctattgcg ggcttacttg cgcttttcgc aggtaatgcc gctgcaaccg
      181 atggtgaaat agtttttgat ggcgagattt taaaatccgc ttgtgaaatc aatgactctg
      241 ataagaaaat tgaagttgct cttggtcact ataatgccga acagtttcgt tctgtaggcg
      301 atcgtagccc aaaaattcca ttcactattc ctttagttaa ctgcccagtg actgggtggg
      361 agcacgataa cggcaacgtt gaagcgtctt tccgtttatg gctggaaacg cgggataatg
      421 gtaccgtccc taacttccct aacttggcaa aagtgggctc ttttgctggc acagcagcaa
      481 caggtgtggg tatccgtatt gacgatgcgg aaagcggaaa ccttatgcca ctaaatgcta
      541 tgggcaatga taacacagtt tatcagatcc ctgcggactc cgctggaatt gtcaatgttg
      601 acctcatcgc ttactacgta tcaaccgttg aagcatccga aatcacccca ggggaagcag
      661 acgctgtcgt taacgtaacg ctggattacc gttaatacgt tacggcgtta tctgaccttt
      721 cagataacgc ccttttcctt cctatttctc gttgtatcag gttgaaaaat aactatgtca
      781 aaacgaacat tcgcgatgat atttaccttg ttgtgtagct tctgtatcgg ccaggcgctt
      841 gcagggggta tcgttttaca gcgaacgcga gtgatctatg atgccagccg caaagaggct
      901 gcgttacctg tcgcaaacaa aggcgcagaa acaccttatt tactgcaatc atgggtagat
      961 aatatagatg gtacaagccg tgccccattt attataaccc caccgctaat tcgtcttgaa
     1021 gctggcgatg attcatcact gcgaattatt aaaacagctg ataacctgcc tgaaaataaa
     1081 gagtcgctgt tctacattaa tgttcgtgcc attccagcaa agaaaaaatc agatgatgtt
     1141 aatgctaacg agttgacgct ggtatttaaa acacggatca aaatgtttta tcgccccgca
     1201 cacctgaagg gacgggtaag cgatgcgtgg aaatcactgg aatttaaacg tagtgatcat
     1261 tcactcaata tatataaccc aactgaatat tacgtcgtat ttgccggact agcagtcgat
     1321 aaagccgatc tcacaagcaa aattgaatat atcgcgcccg gagaacataa acagttacca
     1381 cttccagcat ctggcggaaa gaacgtgaaa tgggctgcga tcaatgatta tggcggcagt
     1441 tccgggacag aaactcgtcc actgcaataa caaatataaa aacacaggtc atcagggaat
     1501 gccacaacga caccaccagg gacataaacg cacaccgaaa cagttggcgc tcattatcaa
     1561 acgctgtttg ccgatggtgc tcactggcag cggcatgctt tgcactaccg ctaacgccga
     1621 agagtattat ttcgacccca ttatgctgga aaccacaaaa agtggtatgc aaacaaccga
     1681 tctgtcacgt ttttcaaaaa aatacgcaca actaccagga acttatcagg ttgatatctg
     1741 gctgaataaa aagaaggttt cacagaaaaa aattacattt accgccaatg cagagcaact
     1801 tctgcagcca cagtttacgg tagaacaact acgtgagctg ggtattaagg tggatgaaat
     1861 cccggcgctg gctgaaaaag atgacgatag cgtgatcaac tcgcttgaac aaatcattcc
     1921 cggtacagct gctgaatttg atttcaatca tcagcgactt aatttgagca ttccccaaat
     1981 tgcactgtac cgtgatgcaa gaggttacgt ctccccttct cgttgggacg atggtatacc
     2041 aacgctgttt accaactact cgtttacagg ttctgataac cgttaccgcc agggcaatcg
     2101 tagccaacga cagtacctaa atatgcaaaa tggtgccaat tttggcccct ggcgattacg
     2161 taactattct acgtggacac gcaacgatca ggcgtcaagc tggaacacta tcagtagtta
     2221 tttacaacgt gatatcaagg cgttgaagtc tcagttgctt ctgggagaaa gcgccaccag
     2281 cggcagtatt ttttccagct acaactttac tggcgtgcaa ctcgcttccg acgataatat
     2341 gttgccaaac agccagcgcg gatttgcccc aacggtacgc ggtatcgcaa acagtagtgc
     2401 aatcgtgact atcaggcaaa atggttatgt gatctatcaa agcaacgtgc cagcgggtgc
     2461 ctttgaaatt aacgatctct acccctcttc caacagcggc gatttagaag tcacgattga
     2521 agaaagtgac ggtacgcaac gtcgctttat ccagccttat tcttcattac ccatgatgca
     2581 gcgacctggg catctaaaat atagcgcgac cgctggacgc tatcgcgctg atgcaaacag
     2641 tgatagcaag gaacccgaat ttgctgaagc cacggcaata tatggtttga ataatacttt
     2701 tacgctgtat ggcggcctgc tcggttctga agattattat gcgctgggga tcggtatcgg
     2761 cggcacactt ggcgcactgg gcgcgttgtc gatggatatc aacagagctg acacccaatt
     2821 cgataaccag cactcttttc atggctatca atggcgtacg cagtacatca aagatatccc
     2881 ggaaaccaac accaatatcg ctgtcagcta ctatcgctat accaacgatg gctattttag
     2941 ttttgatgaa gccaataccc gcaattggga ctataacagt cgccaaaaaa gtgaaattca
     3001 attcaacatc agccagacaa tatttgatgg ggtaagtctg tatgcctccg gttcacagca
     3061 agactattgg ggcaataacg agaaaaacag gaatatctct gttggggttt ccggccagca
     3121 atggggaatt ggttacagcc tgaattatca atacagccgc tacactgatc aaaataatga
     3181 ccgcgcactc tctttgaatc tcagtattcc gttagaacgc tggttaccgc gtagccgggt
     3241 ttcctatcag atgaccagcc agaaagatcg cccaacccaa catgaaatgc gtcttgatgg
     3301 ctcactgctg gatgatggtc gcctgagcta tagtctggaa caaagtctgg atgacgataa
     3361 caaccataac agtagcgtga acgccagtta ccgttcacct tatggaacct tcagtgccgg
     3421 atacagttac ggtaatgaca gtagccaata caattacggc gttaccggcg gcgtggttat
     3481 ccatcctcat ggtgtgacgc tctcgcaata tctgggcaac gcttttgcgc ttattgatgc
     3541 taacggggca tctggcgtga ggatacaaaa ctatccgggg attgctactg atccctttgg
     3601 ctatgcagtg gttccttatc tcacaactta tcaggaaaac cgtctctcgg tagatactac
     3661 gcagctgccc gataacgtcg atcttgaaca aacaacacag tttgtggtgc ccaacagagg
     3721 tgcaatggta gcggcgcgtt tcaacgccaa tatcggttat cgcgtacttg ttacagtcag
     3781 cgatcgcaac ggtaaaccgt tgccctttgg cgctcttgcc agcaacgatg atacggggca
     3841 acaaagtatc gtcgatgagg gcggcatact atatctctct gggatatcga gtaaatcaca
     3901 aagctggact gtacgctggg gaaatcaggc agatcaacaa tgtcagtttg cttttagtac
     3961 accggattca gaaccaacaa cctctgtatt acaaggcaca gcgcagtgcc attaaggata
     4021 aaaaaatgaa aagagcgcct cttataacag gacttttgtt gatatccaca tcctgcgctt
     4081 atgcctcctc agaagggtgt ggagctgaca gcactagcgg tgcgacaaat tacagcagtg
     4141 tggttgatga tgttacggtg aaccagacag ataacgtgac aggacgggag tttacctctg
     4201 caacgctaag tagcactaac tggcaatacg cctgttcctg ctctgcgggt aaggcagtta
     4261 aacttgtcta tatggtcagc cccgtactta ccaccactgg acatcagaca ggatattaca
     4321 aactcaatga cagcctggat attaaaacca tgttattaag gttaattttg gattcatcaa
     4381 tggtcagaag tttaccaccc gcaatgccat gccagacggt tttactccag tagactttga
     4441 tatcacttat gactgtggtg atacttcaaa gattaaaaac tcgttgcaaa tgcgcatcga
     4501 cggtacaact ggggtagtag accagtacaa cctggtcgcc agacgaagaa gttcagacaa
     4561 tgcgcccgat gtcggtattc gtattgaaaa tctcggcggc ggagttgcaa atattccttt
     4621 tcagaacggt atccttcccg ttgatccttc cgggcatggc accgtcaata tgcgcgcctg
     4681 gccagttaat ctggtcggtg gtgagctgga aacaggaaaa tttcagggca cagccaccat
     4741 taccgtcatc gtgcggtaaa agaagacgtt gcgttttgat taacgcaacg cttcggcact
     4801 cttacccctg ctgctccagc gcatacttat acagcgcatt tttcttcacg ccgtgaattt
     4861 ctgcggccag cgccgccgct tttttcagcg gcagttctgc ctgtagcagc gccagcgtgc
     4921 gcagggcatc ggcgggtaag tcttcttcct gtgctttatg accttcgaca atcagcacca
     4981 tttcgccttt gcgacggttt tcatcttcct ttacccacgc cagcagctcg ccaacgggcg
     5041 cgccgtgaat ggtttcccag gttttggtca gctcacgcgc cagaaccacg tagcgggatt
     5101 cgcctaatac cgcaacgata tcttccaggc tatctaacag acggtgggta gattcataaa
     5161 aaatcagcgt gcgcggctcc gcttcaatgg cttttagcgc atcacggcgg ccttttgatt
     5221 tggcaggtaa aaagccttcg taacagaaac ggtcagaggg taaacccgcg gcgcttaacg
     5281 cagtgatagc agcacacggt ccgggtagcg gcaccacacg aatccccgct tcacggcagg
     5341 tacgcaccag atggtagcca ggatcgttaa ttagcggcgt tccggcatcg gaaaccagcg
     5401 caatgttttg cccctcttgc agcttcgcca gcagcgtttc ggctttttgt tgttcgttat
     5461 ggtcgtgcag cgcaaacaac cgggcattaa tcccaaaatg ttgcagcaat aaaccggtgt
     5521 gacgagtatc ctcggcggca atcagatcaa cggcctgtaa tacctctaac gcacgctggg
     5581 tgatatccgc cagattgccg attggcgtcg gtacaatgta aagctggccc tgagaattat
     5641 ccgccgattg gtgttgtttc attgtgtcgt ccgtattgcc gatttaatat tgagcattgc
     5701 gtaaaaaaaa tatcactgga tacattatgg taccctcaac attttctcgt ttgaaagccg
     5761 cgcgttgtct gcctgttgtt ctggcagccc tgattttcgt cggttgtggc acccatactc
     5821 ccgatcagtc cactgcttat atgcagggca cggcgcaggc tgattctgcc ttttatcttc
     5881 agcagatgca gcaaagctct gatgatacca ggatcaactg gcaattactc gccattcgtg
     5941 cactggtgaa agaaggtaaa accgggcagg cggttgagtt gtttaaccaa ctaccgcaag
     6001 aactgaacga ttctcagcgt cgcgagaaaa cactgctggc ggtagagatt aaactggcgc
     6061 agaaagattt tgctggcgcg caaaacttgc tggcgaaaat tacacccgcc gatttagaac
     6121 aaaaccagca agcccgttac tggcaggcaa aaatcgatgc cagccagggg cgtccttcca
     6181 ttgatttact gcgcgcgtta attgctcagg aaccgctgct tggcgcgaaa gaaaaacagc
     6241 agaatattga tgccacctgg caggcgctct cctccatgac tcaggaacag gcgaatacgc
     6301 tggtgatcaa cgccgacgaa aatattctgc aaggctggct ggatctgcag cgcgtctggt
     6361 ttgataaccg taacgatccc gacatgatga aagccgggat cgccgactgg cagaaacgtt
     6421 atccgaacaa tccgggcgcg aaaatgctgc caacgcagtt ggttaacgta aaagcgttta
     6481 aaccagcctc gaccaacaaa atcgccctgc tgttgccact gaatggccag gcagcggtat
     6541 ttggtcgcac tattcagcaa ggctttgaag cggcgaaaaa tatcggcact cagccagtgg
     6601 cggctcaggt agctgccgca cctgccgcag acgtagcaga acaacctcag ccgcaaactg
     6661 cggatggcgt tgccagcccg gcacaagcct cggttagcga tctgaccggt gatcagcctg
     6721 cggcccagcc ggtgcctgta agcgccccgg cgacaagcac cgcagcggta agcgcacccg
     6781 caaatccatc cgcagagctg aaaatctacg atacctcatc acaaccactt agccagatct
     6841 taagccaggt tcagcaggat ggcgcgagta ttgtggttgg tccgttgctg aaaaataacg
     6901 ttgaagagtt gctgaagagc aacactccgc tgaacgtact agcactgaac cagccggaga
     6961 atatcgaaaa tcgcgtcaat atttgttact tcgcgctttc accggaagac gaagcgcgcg
     7021 atgcagcgcg tcatattcgt gaccagggta aacaagcgcc gctggtgctg atcccacgca
     7081 gttcattggg cgatcgcgta gccaatgcgt ttgcgcaaga gtggcagaaa ttgggcggcg
     7141 gcaccgttct gcaacaaaaa tttggttcca ccagcgaatt acgcgcgggt gttaacggcg
     7201 gttctggtat tgctttaacg ggtaccccga ttactcccag agcgacaacc gactccggca
     7261 tgacgaccaa caatccaacg ctgcaaacca cgccaaccga tgaccagttc accaataatg
     7321 gcggtcgtgt cgatgcggtg tacattgtgg caacgccggg tgaaatcgct tttattaaac
     7381 cgatgatcgc catgcgtaac ggtagccaga gcggtgcaac gctgtacgcc agctcccgca
     7441 gtgcgcaagg gaccgctggc ccggatttcc gtctggagat ggaaggcttg cagtacagcg
     7501 aaatcccgat gctggcgggc ggtaatctgc cgttaatgca gcaggcactc agcgcggtga
     7561 ataacgatta ttcactggct cgcatgtatg cgatgggcgt cgatgcctgg tcgctggcaa
     7621 atcatttctc acaaatgcgc caggttcagg gttttgaaat caacggtaat accggaagcc
     7681 tgacggctaa cccggattgc gtgattaaca ggaagttatc atggctacag taccaacaag
     7741 gtcaggtagt ccccgccagt taaccaccaa acagaccggc gatgcgtggg aagtacaagc
     7801 gcgtcgctgg ctggaaggca aaggactgcg atttgtcgcc gctaacgtga acgagcgtgg
     7861 cggcgagatc gatctgataa tgcgtgaagg ccggaccacc gtttttgtcg aggtgcgtta
     7921 ccgccgttct gcgctttatg gcggcgcggc agccagtgtg acccgcagca aacaacacaa
     7981 attattacag actgcccgct tgtggctcgc gcgtcataat gggagttttg atactgtgga
     8041 ttgccggttc gatgtggtag ccttcaccgg gaatgaggtt gagtggatta aggatacctt
     8101 taatgaccac tcataattaa ggtttaagga ttagcgtgca agaaagaatt aaagcttgct
     8161 tcactgaaag cattcaaact caaattgcgg cggcagaggc gcttccggat gccatctccc
     8221 gtgcggccat gacgctggtt cagtctctgc tcaatggcaa caaaatcctc tgttgtggta
     8281 atggaacttc cgctgccaat gcacagcatt ttgctgccag catgatcaac cgtttcgaaa
     8341 cggagcggcc cagcttacct gccattgcac taaataccga taatgttgtc ttaacggcga
     8401 ttgccaacga tcgcttacat gatgaagtgt atgcaaaaca ggtgcgggcg ctggggcatg
     8461 cgggagatgt attgttagcc atttccaccc gtggcaacag ccgcgatatc gtaaaagcag
     8521 tagaagctgc cgttacgcgt gatatgacca ttgtggcatt gaccggttat gacggcggtg
     8581 aacttgcagg tttgttaggg ccacaggatg tggagatccg catcccttcg catcgcagtg
     8641 ctcgcattca ggaaatgcat atgctgacgg taaattgcct gtgcgatctg atcgataaca
     8701 cgcttttccc tcaccaggat gattaaggag aatacatgaa ggcattatcg ccaatcgcag
     8761 tccttatttc cgcgctgctg ttgcaaggtt gtgttgccgc tgccgtagtg ggtactgctg
     8821 ctgtgggtac caaagccgca actgacccac gcagtgtcgg cacccaggtg gacgatggta
     8881 ccctggaagt gcgcgtgaac agcgcattgt cgaaagacga acagattaag aaagaagcgc
     8941 gcattaatgt aacggcctat cagggcaaag tgctgctggt tgggcagtca ccaaacgctg
     9001 aactttcggc tcgcgccaaa cagattgcta tgggcgtaga cggtgccaac gaagtgtata
     9061 acgagattcg tcagggccag ccgattggtc tgggcgaagc gtctaacgat acgtggatca
     9121 ccaccaaagt gcgttcgcag ctcttaacca gcgacctggt gaaatcgtcc aacgtgaaag
     9181 tgaccaccga aaacggtgaa gtgttcctga tggggctggt gactgaacgt gaagcgaaag
     9241 cggcggcaga tattgccagc cgggtgagcg gcgtgaagcg ggtaactacg gcgtttacgt
     9301 ttattaaata gcagcccctt gtaatgcctg atgcgacgct tgccgcgtct tatcaggcct
     9361 acaacgatgc aaaccgtagg tcggataagg cgtttacgcc gcatccgaca tgttttccct
     9421 caaatcagaa taacagcgcc agcccgccga caatcacgcc tgacactgcc accatcgccc
     9481 ccgtcagcca taaggctttc gccgggaacg ctttgcgcag cataatcagt gacggcaaac
     9541 tcaccgccgg gagcgtcatc aacaatgcca gcgccggagc agttcccata cctgccagca
     9601 tcatcgtttg tacaatcgga atttctgctg ccgtgggaat gacaaacaag caccctgcta
     9661 ccgccatcgc caccacccac atcaggctgt tatcgacagc accatcggca tgggggaata
     9721 accagacgcg agcggcaccc aacaccagta ctgcaaggat gtaaaccggg atcgtactcc
     9781 agaaaagcgt ccatagcgcc ctgccccagc gactaaaaaa tccgccctgt gcttccggta
     9841 tgtcaatttc gaccggtgct tgcgtttgcg gtgtttcacg cacccatttt tgcaccagcg
     9901 tcgcaatcag caacaccatc accagcccgg ccaccagacg aatcgccgca aaaccccagc
     9961 cgaggacaaa ccccataaac accagcgtcg ccgggtttaa caccggattg cccatccaga
    10021 atgccagcgc accgcccatc gacacctgtt ggcgacgcat tcccgccgcg accggagccg
    10081 cacagcaggt acacatcatg cctggcagcg aaaacagcgt tcccagcagc gtgccgcgaa
    10141 agcgcgattg cccaagcgta cgcaacaacc agtcacgcgg gatcagcacc tgaatcaacg
    10201 aaccgaggat cacccccagc accgccgctt tccataccgc gaggaaatag atcatcgcgt
    10261 aatccaacgc cgcctgccat ggattagcat ccgcctgcgc aaggatagat ttaccgatac
    10321 tgtgggtttc ggcagcagta aacgctttgc cgtagtaagg ttgccatttc acataccaga
    10381 gaccggcaat gacaacgaga aagaaaagcg cgggtttcca ccactgaatg ggcgttgccg
    10441 cctgagatga agactgacca gtcatagcat tccccaggaa taagttatga taattgagcg
    10501 cgtgaatatt acgctcacta tcaattcttg ggaataatta ttcagctctt ttgcgtaatt
    10561 ctgaagagct taaaatcgtc acaccttcat gctcgggccg catcgcctct gccagcatga
    10621 cccgcgccac gtctctcgca tcaatggatt tccagttacc gggtaacaaa cggaacagcg
    10681 gcgcaaaaag cgtttcgttc atccgctgtt tgctacgatc gcccagtaac atcgacgggc
    10741 gagcaatggt caatttcggc cagttctggg cgattaatgc ttcttccatc tcccctttga
    10801 cgcggttata gaaaaacggc gagtgggcat tggcacccat agcactgacc accagcatat
    10861 gctgcgcgcc cagtcgccgc ccggttaatg cggtatccac taccagcgtg taatcggcat
    10921 gaataaacgc ctctttgctc cccgcttctc gccgcgtggt gccgagacaa caaaacacaa
    10981 tgtcgatggg atcggtgacc tgcgccagcg cgtcagtcag ttgcggatca tggggattaa
    11041 ataccccggg catatcgccc aacggacgtc gcgtcggcgc ggctatggcg ttaactttcg
    11101 gttcgttaat caacatccgc agcaggtgac cgcccaccag ccccgttgcg cctgtaatca
    11161 gtacctgact catcttcgct cctttacaga attgtctgcc ttgcgctcca cggctcatgc
    11221 accaggctta atagaccatg aggtaattat ccccgattgt gggaaattcg cctcatccaa
    11281 tgcaacaacg cggaggaagc atgagtaaga aaattgccgt tttaatcact gatgaatttg
    11341 aggattcaga atttacttca cccgcagacg agttccgtaa agccggacac gaagtgatta
    11401 ccattgaaaa acaagcgggt aaaacggtga aaggcaaaaa aggagaagcc agcgtgacca
    11461 tcgataaatc catcgatgaa gtgacgccag cggagtttga tgccctgttg ctaccgggcg
    11521 gtcattcacc ggattatctg cgtggcgaca accgttttgt cacctttacc cgtgattttg
    11581 tgaatagcgg caaacctgtt ttcgccatct gtcacggtcc gcagctgctg atcagcgccg
    11641 atgtgattcg ggggcgcaaa ctgaccgcgg ttaaaccgat cattattgat gttaaaaacg
    11701 cgggcgcgga attttacgat caggaagtcg tggtcgataa agatcagctg gttaccagcc
    11761 ggacaccgga cgatctgcca gcgtttaacc gcgaggcgtt acgcctgctc ggtgcctgag
    11821 ccgcgtagcc aaatcatttt tttaccaaag cccagcgtgt tgtcggtgaa tttgatttca
    11881 tcgggccgga tttcccacac cggggccgac agcattctgg caaccggaaa gcgacgattg
    11941 tacgccttgc gcgcgaggtc gctttcctca ccttccagcc tgcggatctc acctttaaac
    12001 tgcacgccgc gaattaacgc taccgttttc ggctgaccgt ttaccgttcc ggcaaccgca
    12061 gcctgcggcc cactcatctg cgcgtggcgc gttttttctt ccgtcaaaat gtagaaggcg
    12121 actttctgca catcaaacag ataaaaggca ttggcgcacc agagttcccc ttcctgctgc
    12181 acacaccagg tgacaacatg ttgttttgcc agccaacggc tgatggcggt gagtgtttcc
    12241 attgctgttc tcttttatac tgtgggcctg accttaacat gcgacgcaga cgatgacacc
    12301 ctggtttctt tacttgatcc gtaccgccga caataagctt tataccggga tcaccacgga
    12361 tgtcgaacgc cgctatcagc agcaccaaag cggcaaaggg gcgaaagcac tgcgcgggaa
    12421 aggagaactg acgctggcgt tttctgcgcc agtgggcgat cgttcgctgg cgttacgggc
    12481 ggaatatcgc gttaagcaac tgaccaaacg gcaaaaagag cgtctggtag cggaaggcgc
    12541 agggtttgca aagctgttaa gcagtctgca aaccccggag attaaaagcg attgaaatgc
    12601 tcgtgatact caaccagacc agttacgcca ttcaacgcgt catccgctaa acgatgtacc
    12661 tggaaggcgc tttcagtgcc cggccagcgg caacgcagat cgtgatgcgc cgccagttca
    12721 aagccgaaac ggctgtatag cgccggatcg cccagcgtca ccactgcggc atagccgaac
    12781 tcattaagcg aatcgagtcc ttcatagacc agttggcgtg ccagcccctg tccacggtat
    12841 ttttcatcta ccgccagcgg tgccatgccg acccattgca ggtcttcgcc ctgcacatca
    12901 accggactaa atgccacata gccaatgacc tgaccttcgt catctgtcgc caccagcccc
    12961 agcgtcagaa agccatcttc acgcagatcg tgaaccagct tcgcttccgc atcactttcg
    13021 aatgaacgac gcagcagggc atcaataccc ggcgcatcaa tgggaatttc tactcgaatt
    13081 agcatggttc acctaccgat gtctgtttgg tttcaggcgc ggttttcatt cccgcctcaa
    13141 caaaatccgc cagttgcagc agcatcatgc gtaaggcttt cggcatttgc tccagttcaa
    13201 tggcgtccat caggtttttc acatacagcc ccagctccgt atcgccttca atcaccagcc
    13261 gacgctggaa gaagagcgta tccggatctt gtttacgcgc cgcaatcatc agcagatcgc
    13321 tggcgtcggc actaaaactc acatcagctt gcgcgttctg gctgaccacc agtttgccat
    13381 tcaccaccga ggtaaaccat tgcaggtcaa tatcacgcac atgaatactt aaccagcggc
    13441 cttcaagaaa ctccagctca ccatcatcca gcgcctggcg gaattgccag cttaagacct
    13501 gctcaagaac ctggcgtttt agcgcaaatg gcgtcagttt taccggtaca ctcaacagag
    13561 atggccccaa atgcacaata cgggaacgca gtttatccaa cacgagtttt actccctgtt
    13621 tcaacaatca tcctattttg ccatatcaga aaaataacat agcggtataa atcaacaatt
    13681 ccatatgaaa ttgctgctac caccaataca actttaactg ccttaaatca aaaattgtcg
    13741 cagcaaggtt aactaaaatc ccagttcgtt aacatttttg cgttttgata gcgcaacctt
    13801 caggaaaaat tatggagctg ctctgccctg ccggaaatct cccggcgctt aaggcggcca
    13861 tcgaaaacgg cgcagatgct gtttatatcg ggctaaaaga tgataccaat gcccgtcact
    13921 tcgccggcct taactttacc gagaaaaaat tgcaggaagc ggtgagtttt gtccatcaac
    13981 atcgccgcaa acttcacatc gcgattaaca cttttgcgca tccggacggt tacgcccgtt
    14041 ggcagcgtgc cgtggatatg gcggcgcagc tgggtgccga cgcgctgatc ctcgccgacc
    14101 tcgccatgct agagtacgcc gccgagcgtt acccgcatat tgagcgtcat gtgtcagtgc
    14161 aggcttcggc gaccaatgaa gaggcgatta acttttatca tcgccatttt gacgttgctc
    14221 gcgtggtgct gccgcgcgtg ttgtcgattc atcaggtgaa acaactggca cgggtcacac
    14281 ctgtcccgct ggaagtcttt gctttcggca gcctgtgcat tatgtcggaa ggtcgttgct
    14341 atctgtcgtc gtatctgacg ggtgagtcgc ccaacaccgt gggcgcgtgt tctccggccc
    14401 gtttcgtgcg ctggcagcaa acgccgcagg ggctggaatc ccgcctgaac gaagtgctga
    14461 tcgaccgtta tcaggacggc gaaaacgcag gttatccgac gctatgtaaa gggcgttatc
    14521 tggtggacgg cgagcgctat cacgcgctgg aagaaccaac cagtctcaat accctggaac
    14581 tgctgccgga gttaatggcg gcgaatattg cttcggtgaa aattgaaggc cgccaacgta
    14641 gcccggcgta tgtcagccag gtggcgaaag tctggcgtca ggctatcgac cgttgtaagg
    14701 ccgatccgca aaacttcgta ccgcaaagcg cgtggatgga gacgctcggg tcgatgtccg
    14761 aaggcacgca gaccaccctt ggcgcgtatc accgtaaatg gcagtgagaa gagcaatgaa
    14821 atattcctta gggccagtgc tgtggtactg gccaaaagag acgctggaag atttttatca
    14881 gcaggccgcc accagcagcg ccgacgtgat ttatcttggc gaagcggttt gcagcaagcg
    14941 ccgggcaacc aaagttggcg actggctgga gatggcaaaa tcgctcgccg gaagtggtaa
    15001 gcagattgtg ctctccacgc tggcgctggt gcaggcatca tctgaactgg gcgaactgaa
    15061 acgctatgtt gagaacggtg agtttctgat tgaagccagc gatctcggcg tggtgaatat
    15121 gtgcgctgaa cgcaaactgc cgttcgtcgc cggacacgcg ctgaactgct ataacgcggt
    15181 gacgctgaaa atattgctca aacagggcat gatgcgctgg tgtatgccgg tggagctttc
    15241 ccgcgactgg ctggtgaatc tgcttaatca gtgcgatgag ctgggcattc gcaaccagtt
    15301 tgaagttgaa gtcctgagct acggtcatct accgctggcc tactccgctc gctgctttac
    15361 cgcgcgttcg gaagaccgcc cgaaagatga gtgtgaaacc tgctgcatta agtatccgaa
    15421 cgggcgcaac gtgctgtcgc aggaaaacca acaagtgttt gtactcaatg gcattcagac
    15481 catgagcggc tacgtttaca acctcggtaa cgagctggca tccatgcagg gcctggttga
    15541 tgtggtccgc ctgtcaccgc agggtactga cactttcgcg atgctcgacg ccttccgcgc
    15601 taatgaaaat ggcgctgcac cactgccgtt gacggcgaac agcgactgta acggctactg
    15661 gcggcggctg gcaggactgg agctgcaagc ctaagtaaat agctcacttt gttaacaact
    15721 ttaactactc tttaatgcag tattaaagat taatcggtaa caaagtgagc tgttatgact
    15781 gataaaacca ttgcgttttc gctactcgat ctggccccca ttcccgaagg ttcttcagcg
    15841 cgagatgcat tctcccactc tctcgatctc gcccgtctgg ctgaaaagcg cggctatcat
    15901 cgctactggc tggcagaaca ccacaatatg actggcattg ccagtgctgc cacgtcggta
    15961 ttgattggct atctggcggc gaataccacc acgctgcatc tggggtctgg cggcgtgatg
    16021 ttgcctaacc actcaccgtt ggtcattgca gaacagttcg gcacgctcaa tacactctat
    16081 ccggggcgaa tcgatttggg gctgggtcgt gctccgggta gtgaccagcg aaccatgatg
    16141 gcgctacgtc gtcatatgag cggggatatt gataatttcc cccgcgatgt cgcggagctg
    16201 gtggcctggt ttgacgcccg cgatcccaat ccgcatgtgc gcccggtacc aggctacggc
    16261 gagcaaatcc ccgtgtggtt gttaggctcc agcctttaca gcgcgcaact ggcggcgcag
    16321 cttggtctgc cgtttgcgtt tgcctcacac ttcgcgccgg atatgttgtt ccaggcgctg
    16381 catctttatc gcagcaactt caaaccgtcg gcacgactgg aaaaaccata cgcgatggtg
    16441 tgcatcaata ttatcgctgc cgacagcaac cgcgacgctg aatttctgtt tacctcaatg
    16501 cagcaagcct ttgtgaagct gcgccgtggc gaaaccgggc aactgccgcc gccgattcaa
    16561 aatatggatc agttctggtc accgtctgag cagtatggcg tgcaacaggc gctgagtatg
    16621 tcgctggtgg gcgataaagc gaaagtgcgt catggtttgc agtcgatcct gcgcgaaacc
    16681 gacgccgatg agattatggt taacgggcag attttcgacc accaggcgcg cctgcattcg
    16741 tttgagctgg cgatggatgt taaggaagag ttgttgggat agtgtgtctt aacgcgggaa
    16801 gccttatccg agctggcaac gctgtcccac gtaggcctga taagcgaagc gcatcaggca
    16861 ttgtgtaggc agcagaaatg tcggataagg cccgctgatt actgatacac cggcagcaaa
    16921 ttaaagctcg ataagatatg gaccagtgcg ttgccaacgc caaataccag aatcagcgta
    16981 atcatcggct tgccgcccca gacgcggaat ttcgggctgc caaagcgttt acgcgatgcg
    17041 cgggccagca gtgccggaac aattgccgcc cagatggtag ctgctaaacc agcataacca
    17101 atggcgtaca ggaatccgtt cgggaacagc agaccaccca caacaggagg ggcaaaggtc
    17161 agcaatgccg ttttcaagcg gcccacagcc gagtcgtcga aaccaaacag atctgccaga
    17221 tagtcaaaca aacccagcgt tacgccgagg aacgaactcg ctaccgcaaa gtttgagaac
    17281 acgaccagca gcagatccag actacggctg ttcagtacgc cgcttaacgc ctgtaccagc
    17341 acatcaatat taccgccctt ctctgcaata tcgataaact ccggacgcgg gatgttaccc
    17401 atcgtcgcca gcaaccagat ggtatacagc gccagcgcca tcagcgtacc gtacaccaga
    17461 catttcacga tggttttcgg atctttgccg taatacttca tcaggcttgg cacgttaccg
    17521 tgataaccaa acgatgccag acagaacggc aaggtcatca acagatacgg tgcataagac
    17581 gcattgcttt cagcgacgtt gaacaatgtc gcaggctgca catgccccag caggctaccg
    17641 aaggtgagga agaaggtaat gactttcgcc cccaatacaa tcgctgtcat gcgactgacg
    17701 gctttagtgc tcaaccacac cacaaacgct accagcaatg caaaaccaaa acccgccgcc
    17761 cgtgccggga cgtttagtga catctctgcg aaggtgtgat gcagaatcga accactggca
    17821 gaaatatagg cataggtcag gatatagagc acaaaggcaa tggaaatgcc gttgaccacg
    17881 ttccagcctt tgcccagcaa atctttggtg atggtgtcaa aactcgaacc gattctgtaa
    17941 ttcaggttag cttccagaat catcaagccg gaatgcagca tacagaacca ggtaaagatc
    18001 agcgccgcca ttgaccagaa aaaccacgcc ccggacatga ccactggcag agaaaacatc
    18061 cctgcgccaa taatggtgcc gccgataatc accacgccgc caagcagcga cggtgacgtt
    18121 tgggtggtgg ttagtgttgc catgagggct tctctccagt gaaaaatagt gcgactgcgt
    18181 tgttatgtat tgcactgtac cagtacgcga gtacaaaaga cagaaaaaaa gccccgatgg
    18241 ttcaaatcgg ggctgtatat attattttac agactgtgtt cgctgttcag cgatgattac
    18301 gcatcaccac cgaaacgacg acgaccggta gagtcatcac gacgcggagc gcggccttca
    18361 cgacgttcgc cgctaaaacg acgaccatca ccacgaccac cttcacggcg ttcaccgctg
    18421 aagttacgac caccttcacg acgctcgcca ctgaaaccac gaccaccgcc acgacgctct
    18481 ccgccagtat gcggctgtgc atcgcccagt aattgcatat tcatcggctt gttcagaatg
    18541 cgagtacgtg taaagtgttg cagcacttca cccggcatac ctttcggcag ttcgatggtg
    18601 gagtgagaag caaacagctt aatgttaccg atgtaacggc tgctgatgtc gccttcgtta
    18661 gcaatcgcac caacgatatg acgaacttca acaccatcat cgcggcccac ttcaatacgg
    18721 tacaactgca tgtcaccaac atcacgacgt tcacgacgcg gacgatcttc acggtcgcca
    18781 cgcggaccac ggtcgttacg atcgcgcgga ccacggtcat cacggtcacg gaattcacgt
    18841 ttcggacgca tcggcgcatc tggcggaaca atcagagtac gttcaccctg tgccattttc
    18901 agcagtgcgg cggccagagt ttcgagatcc agctcttcac cttctgcagt cggctgaatt
    18961 ttgctcagca gtgcgcggta ttgatccaga tcgctgcttt ccagctgctg ctgtacttta
    19021 gcggcgaatt tttccagacg gcgtttgccc agcagttctg cgttcggcag ttctacttcc
    19081 ggaatagtca gcttcatagt acgttcaatg ttgcgcagca gacgacgctc gcggttctca
    19141 acgaacagca gcgcgcggcc agcacgaccc gcacgaccgg tacgaccgat acggtgaacg
    19201 taagactcag aatccatcgg gatatcgtag ttaaccacca ggctgatacg ctcaacgtcc
    19261 aggccacggg ctgcaacgtc ggtcgcaatc aggatgtcca gacgaccatc tttcaggcgt
    19321 tccagtgtct gctcacgcag cgcctggttc atgtcaccgt tcagcgcggc gctgttgtag
    19381 ccgttacgct caagagcttc agccacttcc agagtcgcat ttttggtacg aacgaagata
    19441 atcgccgcat caaaatcttc cgcttccagg aaacgtacca gtgcttcgtt tttacgcata
    19501 ccccagacag tccaatagct ctggctgatg tcaggacggg tagtcacgct ggactgaatg
    19561 cgcacttcct gcggctcttt cataaagcgg cgggtaatgc gacgaatcgc ttccggcatg
    19621 gttgcagaga acagagcggt ctgatgacct tccgggatct gcgccataat agtttcaacg
    19681 tcttcgatga agcccatgcg cagcatttcg tcagcttcat ccagaaccag accgctcagt
    19741 ttagagaggt ccagagtgcc acgtttcagg tggtccagca gacggcccgg agtaccgaca
    19801 acgatctgcg gcccctgacg cagggcgcgt aattgcacgt cataacgctg gccgccgtac
    19861 agggcaacca catttacgcc gcgcatgtgt ttagagaaat ccgtcattgc ttcagcaacc
    19921 tgtaccgcca gttcgcgggt cggtgctagc accagaatct gtggtgcttt cagctcagga
    19981 tcaagatttt gcagcagagg taaagagaat gctgcagttt ttccgctccc cgtctgggcc
    20041 atacccagta cgtcgcggcc attcagcaga tgtggaatac actccgcctg aattggagat
    20101 ggtttttcgt aacccagatc gttaagggct tcaaggatag gagccttcag gcccagatct
    20161 gcaaaagtgg tttcgaattc agccatgtag tacgtgtgcc tcaaaattaa tggcggccag
    20221 tctacataac tcatcatgaa attgatcagc aattttcatt gaaaagtgtg aaccggctca
    20281 aagtaggtgt attaacgaac aacaacgccc tcacccgtta aggtgatggc aatcaaaaaa
    20341 gattacgggc tgatgtgtac gtcagctatt gctggtccga ttctgccagg tcatcttggt
    20401 cctggcccag gagcgataat tccaacaatg cgtatcggtg ctcaacaaag ttatgaacgt
    20461 tgttggcaac cgccagtttg aacagtgccg tggcgctgtc caaatccccc agacttaggt
    20521 agtacttacc taaatagaag ttggtttcac tgagatgctc agcgagcgag gtgttatccg
    20581 ttgcgtccgc cttgagcctt tccattaacg tttgttcgct aatgttgccc aggtagaact
    20641 cgacaatgtt ccatccccac tgttccttat ccgatttttc gaagtgctgt ttcaacactt
    20701 ctttagcctg cttctcatcg agcttctgct cggcgagata aagccacaga ctacggaaag
    20761 gatcattggg atcgtcttga taaaacgcca gcagatcatc ttgcgctaac ttgtcacgac
    20821 cgccgtaata taatgcgatc ccgcgattca agtgcgcgta gttgtaagtt ggatcaagct
    20881 caagtacaga atcaaacgct tcataggcag catcaaaatt gcctgcctgc gttaaatata
    20941 tgcctaagta attgaatact tcaggcatat ccggtcggat tgccagcgct tgcgaaaaat
    21001 cgttacgcgc taatgccctc agaccgagac tatcatacaa cactccgcgc tcatataaaa
    21061 gctgtgcgcg ttcgtcatcg gttaaagccc gactggcaag gatttgttcc atacgtgcca
    21121 gaatcacttc ctgctgtaaa gtcggttgca atggtaccgc gaggacttca cttttacgcc
    21181 aggaagtatt actgcatcct gcaagcgtaa gtgctgtcgc aacgaaacac cagcgcaaaa
    21241 aaggcttcat ttcccactcc cgaagaccac ggttgaatga acgtcctgtt cccggttgct
    21301 aacaaggcgt cctgcccggt taaaagcccc ccgccgcagc ggagggcaaa tggcaacctt
    21361 actcgccctg ttcagcagcc ggagcttccg gtgctgcagc aggttgagac tgctcagtcg
    21421 cttctttaat gctcagacgg atacggccct ggcgatcaac ttccagaact ttcaccggta
    21481 cttcctgacc catctgcagg taatcggtca ctttctcaac gcgtttgtca gcgatttgag
    21541 agatgtggac cagaccttct ttaccgccgc cgatggcaac aaatgcgccg aagtcaacga
    21601 tacgggtcac tttaccagtg tagacgcggc ccacttcaat ttctgcagtg atctcttcga
    21661 tacgacgaat agcgtgtttc gctttctcgc catcggtcgc tgcgatcttc acagtaccgt
    21721 catcttcgat ttcgatggtg gtgccagttt cttcggtcag ggcacggatc acagagccgc
    21781 ctttaccgat aacgtctttg atcttgtccg ggttgatctt gatggtatgg atacgcggag
    21841 cgaactcaga gatatcgcca cgtggcgcgt tgatcgcctg ttccattaca cccaggatgt
    21901 gcagacgcgc acctttagcc tggttcagtg caacctgcat gatctctttg gtgatacctt
    21961 caattttgat atccatctgc agtgcagaga taccgtcgcg ggaacccgca actttgaagt
    22021 ccatatcgcc caggtgatct tcgtcgccca aaatgtcaga cagtacaacg tagttgtcgc
    22081 cttctttcac cagacccatt gcgatacccg caacggcagc tttgattggc acacctgcgt
    22141 ccatcagcgc cagagacgca ccgcacacgg aagccataga agaagaaccg ttggattcag
    22201 tgatttcaga cacaacacgt acggtgtacg ggaatttgtc catatccggc attactgcca
    22261 gcacgccgcg cttcgccaga cgaccgtgac caatttcacg acgcttcgga gaaccgacca
    22321 tgccggtttc gcctacggag tacggaggga agttgtagtg gaacaggaag gtatcggtac
    22381 gttcgcccat cagttcatca agaacctgcg cgtcacgagc agtacccagc gttgcggtaa
    22441 ccagcgcctg cgtttcacca cgggtgaaca gcgcagaacc gtgagtacgc ggcagcacgc
    22501 cagtacgcac atccagacca cggatcatat ctttttcacg accatcgata cgcggttcgc
    22561 ctgccagtac gcggctacga acaacgtttt tctcgatcgc gtgcagaatt tcacccagtt
    22621 cgttttcgtc cagggtttcg tcttcagcaa gcagcgtcgc gatggtttca gatttgatga
    22681 catcaacctg cgcataacgc tcttgtttgt cggtgatgcg gtaagcatcg ctcagacgag
    22741 cttcagccag tgcagcaacg cgcgcgttca gcgcttcgtt taccggctcc ggctgccagt
    22801 cccaacgcgg tttaccggct tctttcacca gttcattgat gttctgaata acaacctgct
    22861 gttgttcatg accgaacact actgcgccca gcatctggtc ttcgctcagc agttcagctt
    22921 cagattcaac catcagtaca gcggcttcag tacccgcaac aaccagatcc agtttgctct
    22981 ctttcagctc gtcctgagtc gggttcagta cgtactggtc attgatgtaa cctacgcggg
    23041 cagcaccaat cgggccattg aacggaatac cagacagaga cagtgctgcg gaagcaccaa
    23101 tcatcgcgac gatatccggg ttaacctgcg ggttaacaga aaccacggtg gcgataacct
    23161 gaacttcgtt gacgaagcct tccgggaaca gcgggcgaat cgggcggtca atcagacgcg
    23221 cgatcagggt ttcgccttcg cttggacgac cttcacgacg gaagaagcta cccgggatac
    23281 gaccagcagc gtaggtacgc tcctgatagt taacggtcag tgggaagaag tcctgacctg
    23341 gtttggcttt tttctggcca acaacggtaa caaataccgc ggtgtcatcc atgctaacca
    23401 taacagcggc agtagcctga cgagccatca tgccggtttc cagagtcacg gtgtgttggc
    23461 cgtactggaa tttacgaacg atcggattaa gcaatgtaat atcctttctc tttcttagac
    23521 agtaccttac ggcactggtg ttaatacccg atcttctgcg catcctcgcg actaatgaca
    23581 accctaaccc agctctatgt gggtaaagcc tctcattagc cgcgcgaacc tctgcaacgg
    23641 aagatcattc atagcaacaa tacattagtt tccagtgaat tgctgccgtc agcttgaaaa
    23701 aaggggccac tcaggccccc ttttctgaaa ctcgcaagaa ttagcgacgc agacccaggc
    23761 gctcgatgag ccgggtgtaa cgtgctacgt ctttacgttt caggtagtcg agcagtttac
    23821 gacgctgaga aaccatgcgc agcagaccac gacggctgtg gtgatctttt ttgtgctctg
    23881 caaagtggcc ctgcaggtgg ttgatctgtg cagtcagcag tgctacctga acttcggtag
    23941 aaccggtgtc gtttgcgtca cgaccaaact cagaaacgat tttagctgtt gcttcagtac
    24001 ttagagacat tttaaaactc caaagtatat agaatgaaag gacgccgatc tctaattcag
    24061 cgatcccagt gtacgttacg caaagtgtta aacaatttac gcgacgttaa gcggcagtat
    24121 tctactcgta gcgacctgtt atcgcaagac ggttaacatt acgccgggta ttcaaccacc
    24181 agacgacgag gcgcaacgcg gccttcatcg tcaatttcgc ccataccgat aaacttgccg
    24241 ttctcacctt ccgtaacgcg aaccagtcct tccagcggcg cgccagatgt acgaaccggg
    24301 ttaccatttt tgaagtaaac agaagacgtt aacggaagat tcaccaccgg gtagtccgaa
    24361 gctggactgt ccattggcat cagtaatgga tcaagcaact ccgcagccgg aatatcctgc
    24421 tgttcggctt gttcgaccag ttcacgcagg tgctccaggg tcaccatccg ttcaaccgga
    24481 tatttactta ccgccagacg acgcaggtaa atcacatgcg cgccacagcc gagtttttca
    24541 cccaggtcat caatgatggt gcggatataa gtgccttttg agcagtgaat ttccagctcc
    24601 agctcattgc cttcatggcg aataaacagc aattcataaa cggtaattgg acgcgcttca
    24661 cgcggaactt caatgccctg acgcgcatat tcgtacagtt ttttgccctg gtatttcagt
    24721 gctgaataca tcgatgggat ctgttcgata tcgccacgga aagtatccag tgccgccgcc
    24781 agttgttctg cactaaaggt taccggacgc tcttcaacga tttgtccgtc ggcgtcagaa
    24841 gtatcggtac gctgtccaag acgcgcaatg acccgatagc gtttgtcgga gtccagcaga
    24901 tactgggaaa acttcgtcgc ttccccgagg caaatcggca acatgccggt cgccagcggg
    24961 tccagcgcac cggtatgccc ggcacgattg gcgttatata tacgtttcac tttttgcagc
    25021 gcatcgttgc tggacatacc ctgcggttta tccagcaaca aaacgccgtt aatgtcgcga
    25081 ccgcgacgac gaggacgact cattagtcct ccttgctgtc gtccgggtta acacgacgtt
    25141 cttcgtcatg tttgaccacg ctggtcacca ggtttgacat acgcatccct tcaaccagag
    25201 agttgtcgta gaagaaggtc agttccggca cgatacgcag gcgcatcgct ttacccagca
    25261 ggctgcggat gaaaccagaa gcttcctgca acgctttgat gcccgcttta accgcgtctt
    25321 catctttgtc gttgaggaaa gtcacataca ctttggcata cgccaggtcg cgagacattt
    25381 cgacaccgga aacggtggtc atcatgccca gacgaggatc tttaatttca cgctgcagga
    25441 tgagagcgat ctctttttgc atttcctgcg ctacgcgctg cgggcgacca aattctttcg
    25501 ccataataaa ttctcctgac aaaaaagggg ctgttagccc ctttttaaaa attaatttca
    25561 ggtggaaggg ctgttcacgt tggcctgata agacgcgcca gcgtcacatc aggcaatcca
    25621 tgccggatgc ggcgtaaacg ccttatcccg catggaaccc taaaaacctt aagcgatggt
    25681 acgttggatc tcgatgattt cgaatacttc gatcacatcg ccagtgcgga cgtcgttgta
    25741 gttcttaacg ccgataccac attccatacc gttacggact tcgttaacgt catctttgaa
    25801 gcggcgcagg gactccagct cgccttcata gataaccacg ttgtcacgca gaacgcggat
    25861 cgggttgtga cgtttaacca caccttcggt aaccatacag cctgcgatgg caccaaattt
    25921 cggcgatttg aacacgtcac gaacttccgc cagaccgata atctgctgtt tcagttccgg
    25981 agacagcata ccgctcatcg ccgctttcac ttcgtcgatc aggttataga tgacggagta
    26041 gtaacgcaga tccaggcttt ccgcttcaat cactttacgt gcagaggcat cagcacgtac
    26101 gttgaagcca accaggatgg cgttggacgc cgcagccaag gtggcgtcgg tttcggtgat
    26161 accacctacg ccagaaccga tgatcttcac tttaacttcg tcagtagaca gtttcagcaa
    26221 ggagtcggag atcgcttcga cagaaccctg tacgtctgcc ttcaggacga tattcacttc
    26281 gtgaacttcg ccttcggtca tgttggcgaa catgttctcg agtttagatt tctgctgacg
    26341 cgccagttta acttcgcgga atttaccctg acgatagagt gcaacttcac gcgctttctt
    26401 ctcgtcacgt acaacggtaa cttcatcacc cgcagccgga acaccggaca agccgaggat
    26461 ttccaccgga atggacggac ccgcttccag cacttcctga cccagttcgt tacgcatcgc
    26521 acgaacacga ccgtattcga agccacacag aacgatatcg cccttgtgca gagtaccttc
    26581 acgtaccaga acggtagcaa ccggaccacg acctttatcg aggaaggatt cgataaccgc
    26641 accgctcgcc atacctttac gtaccgcttt cagctccaga acttccgcct gcagcaggat
    26701 agcgtccagc agttcatcga taccggtacc cgctttcgca gatacgtgta cgaactggct
    26761 ttcaccgccc cactcttccg gcaggatgcc gtactgggag agttcgttct taacgcgatc
    26821 cggatcggct tctggtttat cgatcttgtt cactgcaacc accaccggta cctgcgccgc
    26881 tttcgcgtgc tggattgctt cgatggtctg cggcattacg ccgtcgtcgg cagcaacaac
    26941 caggactacg atgtcagttg cctgcgcacc acgtgcacgc attgaagtaa acgcggcgtg
    27001 ccccggggtg tccaggaagg tgatcatgcc gttttcagtt tcaacgtggt atgcaccaat
    27061 gtgctgggta atgccgcccg cttcgccaga ggccactttc gttgaacgaa tgtagtccag
    27121 cagagaggtt ttaccgtggt caacgtgacc catgatggtc acaaccggag cgcgcggttc
    27181 agccgcagct cccgtgtcac ggtcgctcat taccgcctct tccagctcgt tttcacgacg
    27241 caggataact ttgtggccca tctcttcagc aaccagctgt gcggtttcct gatcgataac
    27301 ctggttgatg gttgccattg cgcccagttt catcatcgct ttgatgacct gagagccttt
    27361 aaccgccatc ttgttcgcca gttcgccaac ggtgatagtt tcgccgatca caacgtcacg
    27421 gttaacggcc tgagcaggct tctggaagcc ttgctgcagc gaagaacctt tacgttttcc
    27481 gcccttaccg ccacgtactg ctgcgcgtgc ttcttcacga tcagcttttg attcagcgtg
    27541 tttgttgcct ttcttcggac gcgctgcttt cgcgttacga ccacggccac ggccgccttc
    27601 gacttcacga tcgctttcgt cttctgcctg gcgagcatgt tgagaagtag tgacgtgata
    27661 atcgctggaa tcctcagtcg gttccgcgtt atcagtccat ttgttttctt ccgccatacg
    27721 acgtgcttct tcagcaacgc gacgtgcttc ttcttcgagt ttacgacgcg cttcttcttc
    27781 ggctttacgc ttgagctctg cagcttcctg ctcacggcgg gctttttcag cctgggcgtt
    27841 tttagtcata tcgtcttgtt gattgctcac tttgtctttt tccgcagctt cacgtttcgc
    27901 ttgttcagca gcttcacgct tagcttgttc tgcggcctca cgttcagctt tttgttgcgc
    27961 ctcgcgttta gccgattctt ctgcctcacg acgggcttgc tcttccgctt cacgctgcgc
    28021 ttgctcttcc gctgcaaggc gttcagcctc ttgcggatcg cgtttcacaa aggtgcgttt
    28081 cttgcggact tcgatttgta ccgatttgct ttttccaccg gtaccaggaa tgttaagggt
    28141 gctgcgtgtt ttacgttgca gcgtcaattt gtccgggcct gaatttttct gattcaggtg
    28201 gtcaatcaaa gtctgtttct cttgtgcaga cacagagtcg tcagcagact tccggatacc
    28261 tgcatcagca aattgctgta ccaggcgttc cacggaggtc tgtcgctctg cggccagcgt
    28321 tttaatcgtt acatctgtca tgctgttcct tcctgctaca gtttattacg cttcgtcacc
    28381 gaaccagcaa atattacggg cagccataat cagtgctccg gctttttcgt cggtcaaccc
    28441 ttcgatatca gccagatcat caatgccctg ttcggcgaga tcttccagcg tacaaacgcc
    28501 acgggcggcc agtttgaatg ccaaatcacg atctacccct tcaaggttca gcagatcgtc
    28561 agccggtttg ttatcaccga ggctttcttc ctgggcctgt gcaatggtgg ccagtgcatt
    28621 tttagcacgc tcgcgcagtg cttcaacggt cggctcatca aggccttcga tttccaacag
    28681 ctctttcatc ggcacatagg ccagttcttc cagcgtcgag aagccttcct ctaccagaac
    28741 agtcgcgaag tcttcgtcga tgtcgagata tttggtgaag gtgtcgatcg ctgcgtgcgc
    28801 ttccgcctga tgcttagcct gcaggtcgtc aacggtcatc acgttgagtt cccaaccgct
    28861 cagctgcgaa gccagacgca cgttctgacc gttacggcca atcgcctgcg ccaggttacc
    28921 ggcttcaacg gcgatatcca tggtgtgttt atcttcatcc accacgatag aagcaacgtc
    28981 tgccggtgcc attgcgttaa tcacaaactg cgccgggtta tcatcccaca ggacgatatc
    29041 gatacgctcg ccgcccagtt cagtagaaac cgcctgaaca cgcgcgccac gcatacctac
    29101 gcaagcacct accggatcga tacgtttatc gttggttttc accgcgattt tcgcacgaga
    29161 acccgggtcg cgagccgctg ctttaatttc aatcacttct tcgccgattt ctggcacttc
    29221 aatacggaac agttcgatca gcatttccgg cttggaacga gtgacgaaca gttgcgcgcc
    29281 acgcgcttcc gggcgaacgg aataaagcac gccacgaacg cggtcgccag ggcggaagtt
    29341 ttcacgcggc agcatatctt cgcgcaggat cacggcttca gcgttgttgc ccagatccag
    29401 agagatgttg tcgcggttta cttttttcac cacgccggtg atgatttcac cttcgtgttc
    29461 acggaactga tcaaccacca tcgcacgttc ggcttcacgc actttctgca cgataacctg
    29521 ttttgccgtc tgggtggtga tacggtcaaa ggtaacagac tcaatctgat cttcaacgta
    29581 atcgcccagg ttcaggcttt catcttcata acgtgcggct tcaagggtga tttccttggt
    29641 cggctgggtg acttcatcaa caactaacca gcgacggaag gtgtcaaaat caccgctttt
    29701 gcgatcgatc tgtacgcgga cgtcgatctc ttgttcatat tttttctttg ttgctgtcgc
    29761 cagcgcgctt tccaatgctt cgaaaatctt ctcgcgaggt agcgcctttt cattggatac
    29821 ggcttcaact acagccaaaa tttctttgtt catcgcgggc ttttcacctc atccagacta
    29881 ttaaaagtgg ggaaccaagt tcgccttctg gatattactc agcgcgaaca cttcatcttt
    29941 accttcgacg gtaactgtga tcatttcacc gtctaccgct ttgataacgc cctgccattt
    30001 acgacggttt tgtaccgcca tacggagaac cagagtcacc tcttctccga caaaacgggc
    30061 gtagtgttca gccgtgaaca gtgggcgatc gagacccggt gaggagactt ccaggttata
    30121 agcaacggtg atgggatctt caacatccag cacagcactt acctggtggc tcacatcagc
    30181 acaatcatca acattgatgc catcttcact atcaatatag atgcgcagtg tggatgtgcg
    30241 accgcgaata aattcgatgc caaccagttc aaaacccagg gcctcaactg gcgcagtaat
    30301 catctctgtt aatttttgct ctaatgtgga caagcccacc cccaagacat aaaaaaaggg
    30361 cctaaagccc agttattctg tagtcagata acaaaaaacc ccgataaatc ggggctttat
    30421 ataactgaac cctataaccg caactgcggt ctggagcact ttccagaagg attttttcaa
    30481 atcccactac gaaggccgaa gtcttcacag tatatttgaa aaaggactct aagggaaagt
    30541 ggttgcgggg gccggatttg aaccgacgac cttcgggtta tgagcccgac gagctaccag
    30601 gctgctccac cccgcgcctg aaacgtggca aattctactc gttttgggta aaaaatgcaa
    30661 atactgctgg gatttggtgt accgagacgg gacgtaaaat ctgcaggcat tatagtgatc
    30721 cacgccacat tttgtcaacg tttattgcta atcacgtgaa tgaatatcca gttcactttc
    30781 atttgttgaa tacttttgcc ttctcctgct ctcccttaag cgcattattt tacaaaaaac
    30841 acactaaact cttcctgtct ccgataaaag atgattaaat gaaaactcat ttattttgca
    30901 taaaaattca gtgagagcgg aaatccaggc tcatcatcag ttaattaagc agggtgttat
    30961 tttatgacga cgattctcaa gcatctcccg gtaggtcaac gtattggtat cgctttttcc
    31021 ggcggtctgg acaccagtgc cgcactgctg tggatgcgac aaaagggagc ggttccttat
    31081 gcatatactg caaacctggg ccagccagac gaagaggatt atgatgcgat ccctcgtcgt
    31141 gccatggaat acggcgcgga gaacgcacgt ctgatcgact gccgcaaaca actggtggcc
    31201 gaaggtattg ccgctattca gtgtggcgca tttcataaca ccaccggcgg cctgacctat
    31261 ttcaacacga cgccgctggg ccgcgccgtg actggtacca tgctggttgc tgcaatgaaa
    31321 gaagatggcg tgaatatctg gggtgacggt agcacctata aaggaaacga tatcgaacgt
    31381 ttctatcgtt atggtctgct gaccaatgct gaactgcaga tttacaaacc gtggcttgat
    31441 actgacttta ttgatgaact gggcggtcgt catgaaatgt ctgaatttat gattgcctgc
    31501 ggtttcgact acaaaatgtc tgtcgaaaaa gcttactcca cggactccaa catgcttggt
    31561 gcaacgcatg aagcgaagga tctggaatac ctcaactcca gcgtcaaaat cgtcaacccg
    31621 attatgggcg tgaaattctg ggatgagagc gtgaagatcc cggcagaaga agtcacagta
    31681 cgctttgagc aaggtcatcc ggtggcgttg aacggtaaaa cctttagcga cgacgtagaa
    31741 atgatgctgg aagctaaccg catcggcggt cgtcacggcc tgggcatgag cgaccagatt
    31801 gaaaaccgta tcatcgaagc gaaaagccgt ggtatttacg aagctccggg gatggcactg
    31861 ctgcacattg cgtatgaacg cctgttgacc ggtattcaca acgaagacac cattgagcag
    31921 tatcacgcgc atggccgtca gttgggccgt ctgctgtacc aggggcgttg gtttgattcc
    31981 caggcgctga tgctgcgtga ctctctgcaa cgctgggttg ccagccagat cactggtgaa
    32041 gttaccctgg agctgcgccg tgggaacgat tattcaatcc tgaataccgt ctcagagaac
    32101 ctgacctaca agccagagcg tctgacgatg gaaaaaggcg actcggtgtt ctcgccagat
    32161 gatcgtatcg gtcaactgac catgcgcaac ctggatatca ccgatacccg cgagaaactt
    32221 ttcggttatg ccaaaactgg cctgctttcc tcctctgccg cttcaggcgt gccgcagatg
    32281 gagaatctgg aaaacaaagg ccagtaattc gacatcaacc ctgcccttca ggggtggggt
    32341 taccttgctt atcctctgta aactcctttc tgagcatcac ataatcgaaa acatcatggt
    32401 ttgcatccac aaccagtgag tctcctttat agtgtacaat cacttcctca agcgtttgcg
    32461 gctgttccgg ctttgttacc cccatccacg cattaataag gtaattattg taagctgtcg
    32521 aaaagatgtt gttttccgtt gtgcgatcca caccatcccc aagaacgggg ctataccaga
    32581 taaacatcgg aacatgatat gcctgctggc tggcctccct gcctccatga aaatagacgt
    32641 tcttcttcgt agggtcacgt tccaggccgt ggtcggcaaa atacatgacc gaggcgcggc
    32701 gatcttttaa taattcaaaa acctgaccta gcaaactatc tgtgtaatga atggagttgt
    32761 catagcaggc atcctgatcg tcctgcggtt gaaacacggc gctggattgc ggataggcgc
    32821 tacaagccgg ttcatggctt ccatttaaat gaagaacaat cagtttcttt tgctgcgtat
    32881 tttgctgtaa tgcttgcgat aaatgcggca acaacaattc atcaaatcct ctgacataaa
    32941 ctgtttccat ggcacgcatg gcgatactgg taactgctgt accattctgc cgaaaagcgg
    33001 attgcgagct tagccagaaa gtctgaaatc ctgcttgatt agccatatta ataatgttgt
    33061 cggggtaatt atgaatgtca tggctcaaaa cagaatcagc agttaaagaa aggggaaccg
    33121 acagcgcagt gtaaggtgca ccgcttattg cttgattaaa cagtttgatc tgttttcttt
    33181 gtgcttcgac ttgcggtgtc gtagagcgtg tatatccata caaagacata ttgtcgacac
    33241 gtacagactc ccccacaatc aacacgtagg tatcaatacc tgtatccctg actgataatt
    33301 gaaaatacgg cactgtgttt gcaattgaga gtaatctttg atgctctttc gctgctaaag
    33361 caaaataatt gagattgaaa aacggcgtat aggtagcaaa tcgcgacgct agtatatatg
    33421 gactgaacgc atttttattt ttggcatctt tataagcaaa ttgacaagcg gaaaataaac
    33481 ttcccgaaat gacaatcagc aataatattc ctgtcacttt ttttgtcggc aaggaaacat
    33541 catattttat tattacagcc aaaaaaagaa gggataaaaa ggcaaaagca catagataag
    33601 ggctatacat tcccagcatt ttggcaactt catccggatc actctgcagc acactaatcg
    33661 caaatccatc attgaaagtt gtaccaaagg tacaccatga atagaggcta atactcatgt
    33721 ctgccgcagt cagtaacaca aatggtgcgg caacaagcag gcgaagcgga agggatgata
    33781 tacgtttaac aaccaggatg gtcagaacga agaacagcaa tcgctgaacc atatgcgctc
    33841 cggaagaggc gactaagttc gtaataccaa aaacaataac actaagatac aacaaccaat
    33901 gagatttaaa acttctagca aatttgttga atactgtcat ccagaaatcc aagttcacaa
    33961 ccaatataaa caattatcaa caatgtgcaa ttttatatca gacatgtttt tcaactctga
    34021 tccatatcag aacgaaacac tgttagtttt ttctttttaa atgatgcggc cattgcgttc
    34081 tcagtaatta aataacatta tcaatgcgtc tctaaacatt acgcagaacg tagccatcag
    34141 tagttatact gacaggagca ggatggttta tcgagaagta aacatatccg tattcgtacc
    34201 ggaactggag ttgacacaat aaagtgccaa ttatgtcagc agtagggaaa agtttataac
    34261 gaaggcgtac cggcaccatc gtttcaaggt accagctacg agtaaagcaa ctggacgaga
    34321 tacagatacc tgacaaccat tcctcagacc aggaccaaaa cgaaaaaaga cgcttttcag
    34381 cgtctctttt ctggaatttg gtaccgagga cgggacttga acccgtaagc cctattgggc
    34441 actaccacct caaggtagcg tgtctaccaa ttccaccacc tcggcacgga aactactttt
    34501 tagttcggga tatcgctggt cggcttagcc ggagcagctg gctgagtttg ttcggttttc
    34561 gccggcgcac tcaggttttc ccattcgcta cctttattgg tcttgttgct attgatgtta
    34621 cccagcacca gactgatgat gaagaataac gttgccagca acgccgtcat gcgggtcatg
    34681 aagttaccag aaccacttga accaaacagc gtagcggaag cgcctgctcc gaaggaggct
    34741 cccatatcag cgcctttacc ttgctgtagc atgatcagac caacaaggcc aattgccaca
    34801 ataaggaaaa ctaccaaaag agcttcgtac ataatcaacc tgttccttgc ggagttgccg
    34861 cgtaccaatg cttcaaccaa taaagcggga gtttataatc tttcccactg aagcgggtgt
    34921 gaatactaac caaaacgaat gaccttcgca agggcaattt tatcgcattg tatcaactgc
    34981 ggaaaaaaac agcaaaaccg atgtgttcgc cgagaaaaaa ggcgacaatc gccgcctttt
    35041 tagccagtta tctaacgctt taaacggctt ttactgcatc ggcgatgcgg tgtgcaaatt
    35101 cagtcacctg cgcttcgtct tcgccttcca ccatcacgcg aattaacggt tcggtgccgg
    35161 atttacgcaa cagcacacgt ccacggctac ccagcgccgt ttcaacttct gcggtcaccg
    35221 ctttgactga ttcatgctcc agcggatcgc cgctacctgc ggtgtaacgc acgtttacca
    35281 ggatttgcgg gaacattttc atgccgctgc aaaggtcgtg caggctcata tggttacgcg
    35341 ccatcgccgc cagcacctgc aaaccagcaa cgatgccgtc accggtagtg gttttatcca
    35401 gcaggatcac atgaccggaa ttctctgcac cgatacgcca gcctttctcc tgcatttttt
    35461 ccagtacgta gcggtcaccc actttcgcgc gcgcaaatgg aattcctaac tgtttcagcg
    35521 ccagttcaag ccccatgttg ctcatcaatg tacccacagc gccaccacgc agctggccct
    35581 gacgaagacc ttcacgcgcg atgatataca tgatctgatc gccatcgact ttattgcctt
    35641 catggtcaac cataatcacg cgatcgccgt cgccgtcgaa ggcaataccg agatccgctt
    35701 tttcagccag cacacgagct tggagcgcgc gaacgtcggt agcccccact tcggcattga
    35761 tgtttacacc gtttggctca caaccgatag cgataacatt cgctcccagt tcgcgcagca
    35821 cgttcggcgc gatgtgataa gtcgcaccgt ttgcgcaatc caccacaatc ttcagttcac
    35881 tgaggctaag ttcgttcggg aacgtggctt tgcaaaactc gatatagcga cccgcagcat
    35941 caacgatacg gctggcttta cccagttctg ccgaatcaac acagctgatc tctttttcca
    36001 tttccgcttc gatggcttct tcaactgcat ccggcagctt agtaccgtca atagaaaaga
    36061 atttaatgcc attatcgtag aacgggttat gcgatgcaga tatcacaatt ccggcctctg
    36121 cgcggaaggt acgcgtcaga taagccacgg ccggtgttgg catcgggcca gtgaagagtg
    36181 cggaaaggcc cgcagccgcc agccccgctt ccagtgctga ctccagcata tagccagaaa
    36241 tacgcgtgtc tttaccaata ataatcttac gggagccgtg gcgcgccagc actttacccg
    36301 cggcccaacc cagcttaagc acaaaatcag gtgtgatcgg cgcatcccct acacgaccac
    36361 gaatcccatc ggtaccgaaa tatttacgat tactcatagc gtttgttttc ctttgcagac
    36421 agagtagctt ccaccactcg catcgcttct acggtttctt tgacgtcatg aacacgaata
    36481 atgtgcgcgc cttgcattgc ggcaatgacc gcacaggcca gactaccgct caggcgctcg
    36541 gacggcccca cgttcagcag ctgcccaatc atcgattttc gtgacatacc caccaacagc
    36601 ggcaggttga aatggtgaaa ttcagccagg cgcgccagta atgaatagtt atgggagaga
    36661 tttttaccga aaccgaatcc ggggtcgagc aacaatttct cttttgcgat acccgcctgc
    36721 tcgcaacgtg ctatttgctc aataaagtag cgattcactt ctgcaaagac atcgtcatac
    36781 ttcggggctt cctgcatggt ttttggattt ccctgcatat gcatcagaca aaccggtaaa
    36841 ccggtttctg cagccgcctc cagagcgcca ggttcggaaa gggagcggat atcgttaata
    36901 atgtgagcgc caactttcgc tgactcacgg atgacttctg gtttggatgt atcgaccgag
    36961 atccagactt cgaagcgttg agcaattgcc tcaaccacag gaataacacg ttgcaactct
    37021 tcttcaacgc taacttccgc cgcccctggc cgcgtggact cgccaccaac gtcaatgatc
    37081 gtcgcgccag cgttgatcat cagattcgca tgtttcaccg catctatcag cgagttatgc
    37141 gtgccaccat ccgaaaagga atcaggcgtg acgttgagga tccccattac gtgaggatgg
    37201 ctaaggtcca gtgaagtacc ctgggcaaag agtttcatga tgttatccct ggtatgaatt
    37261 gataagaaaa aaccccggag cacgccccgg ggttttcggt acaaatacag tcatctgatg
    37321 cgggaactta cttgtcgcct aactgctctg acatggtgtt acccgggttc ggcgtacgcg
    37381 gttcatcaac cggacgagga gcctttggac taccattgtc gccagcattg ttagaagcgc
    37441 ctggttcttc ccagcccgct ggcggacgta catcgcgacg tgccatcagg tcatcaatct
    37501 gcggtgcgtc gatagtctca tatttcatga gagcatcttt catcgcatgc agaatatcca
    37561 tattgtcggt cagaagctga cgcgcacgat tatagttacg ctcaatcagt gctttcactt
    37621 cctggtcgat gatacgtgca gtttcatcgg acatatgttt cgctttcgct acgctacggc
    37681 cgaggaacac ttcaccttct tcttccgcgt acagcagtgg acccaatttc tcagagaagc
    37741 cccactgagt caccatgtta cgtgccaggt tggtcgcaac tttaatatcg ttggacgcac
    37801 cggtagaaac atgttccggc ccgtagatga tctcttctgc cagacgacca ccgtacagcg
    37861 tagaaatctg gctttccagt ttctgacggc tggcgctgat tgcgtcgccc tcaggcaaga
    37921 agaaagtcac acccagcgca cgaccgcgtg ggataatcgt cactttgtgc accggatcgt
    37981 gctccggcac caggcgaccg ataatcgcat gacccgcttc gtggtaagcc gtcgattctt
    38041 tctgcgcttc cgtcatcacc atggagcgac gttccgcacc catcatgatt ttgtctttcg
    38101 ctttctcgaa ctcaaccatc gacacaacgc gtttgttgcc acgagcagcg aacagtgccg
    38161 cttcgttcac caggttcgcc aggtcagcac cggagaaacc aggagtacca cgggcaatga
    38221 ttgccgcgtc gatatcgggt gccaatggta cgcgacgcat gtgaaccttc aggatctgct
    38281 cacgaccgcg aacatctggc aagccaacca caacctgacg gtcgaaacgg ccaggacgca
    38341 gcagcgctgg gtcgagaacg tccggacggt tagtcgcggc gataacgatg ataccttcgt
    38401 taccttcgaa gccatccatc tcaaccagca tctggttcag agtctgttca cgttcatcgt
    38461 gaccaccgcc cagacctgcg ccacgctggc ggcctacggc gtcgatttca tcgataaaga
    38521 tgatgcacgg tgccgctttc ttcgcctgtt cgaacatgtc acgaacacgg gatgcaccca
    38581 caccgacgaa catttctacg aagtcagaac cggagatagt aaagaacgga actttcgctt
    38641 cgcctgcaat cgctttcgcc agcagcgttt taccggtacc cggaggaccg accatcagga
    38701 cgcctttcgg gatcttaccg ccgagtttct ggaagcggct cggctcgcgc agatactcaa
    38761 ccagttcagc aacttcttct tttgcttcgt cgcagcccgc aacgtcagca aaggtcgttt
    38821 tgatctgatc ttccgtcagc atgcgcgctt tactcttacc aaacgacatg gcacctttgc
    38881 caccgccgcc ctgcatttga cgcatgaaga agatccagac accaatcagc aacagcatcg
    38941 ggaaccagga gatgaagata gaagccagca ggctgggttc ttcaggcggt tcaccgacaa
    39001 ccttgacgtt cttggtcaac aggttatcca gtaatttcgg atcctgaacc ggaatgtaag
    39061 tggtataacg gttactatct ttcttggtaa cgttgatttc acgtccgttg atacgcgctt
    39121 cacgaacctg gtcgttattg acctcttgta ggaaggtaga gtaatccacc ttacggccat
    39181 tagactcgct gggcccaaag ctctggaata ctgacatcag cacaacggca atgaccagcc
    39241 agagtattag gtttttcgcc atgtcactca agggattaac ctcttattac aactgtgtta
    39301 aaaacagcgt caggatactc tatatccagc atctttcaaa ctttcgtctg aaatctcccg
    39361 gttagggttt acgcccggtc gctacaatat acacttcccg cgaacgtgca cgagaagagt
    39421 ccggcttacg aactttgacc ttcgtaaaca gggagcgaat ttcccttaga tactcatcga
    39481 aaccttcgcc ctggaacacc ttcactacaa aactgccacc tggcgctaat acatcacgac
    39541 acatttctag cgccagttcc accagataca tggcacgggg gatatccacc gccggtgttc
    39601 cgctcatgtt tggtgccata tcggacatga caacctggac tttgctgtcg ccaacgcgct
    39661 ccagcagtgc tttcatcacc agttcatcac gaaaatcgcc ctgaagaaag tccacaccaa
    39721 cgataggatc cataggtaaa agatcgcaag cgatgatgcg gcctttgccg ccaatttggg
    39781 tgaccacata ttgtgaccaa ccacccggag cagcaccaag gtcgacaacc gtcattcccg
    39841 gtttaaagag tttgtcactt tgctgtattt catcaagttt aaaccaggca cgggaacgta
    39901 accccttttt ctgtgcctgt tgaacatatt tatcgctaaa gtgttcctga agccagcggc
    39961 tggagctggc agaacgcttc ttacctgtca tttaactttc ccatggggat aactcatcgt
    40021 aaccaattgc gtaaattttt acgcgcctat ttggtgatat atgggagatg gcggtagaat
    40081 gacccgtttt caatcccaac gtaagcaaaa aatacgatga atctgagtac taaacaaaaa
    40141 cagcacctga aaggtctggc acatccgctc aagccagttg ttctgcttgg cagtaatggt
    40201 ttgaccgaag gggtgctggc cgagattgaa caagcgttag agcaccatga actcatcaag
    40261 gtgaaaatcg ccaccgaaga tcgcgaaact aaaaccttga tcgtggaagc tatcgtgcgc
    40321 gaaaccggcg cctgtaatgt acaggtcatc ggtaaaacgc tggtgcttta tcgcccaact
    40381 aaagaacgta aaatctcgct gccacgctaa gattatccta aagttacaca catttgctgt
    40441 gtaaaacgag gggttttccg caggcaggag agcaaaatgc cacgctctgt tcgttgataa
    40501 aaggccgcta tgcggccttt ttcctttctt tacaatacat caacatcttg agtattgggt
    40561 aattcttaca ggtattccac cttaattact tcaaattcta cttcgccgcc cggcgttttg
    40621 atgaccacaa catcatcttc ttctttgccg atcaggccac gagcaatagg tgagtttaca
    40681 gaaatcaggt tttgtttaaa gtcagcttcg tcatcgccaa cgatgcgata agtctgttct
    40741 tcgtcagaat cgagattcag caccgttacg gtagcaccaa aaataacgcg cccattgttg
    40801 ggcattttgg tgacatcaat cacctgcgcg ttcgacagct tggcttcgat gtctttgata
    40861 cggccttcgc agaagccctg ctgttcacga gctgcgtggt attcggcgtt ttctttcagg
    40921 tcgccatgct cacgcgcttc cgcgatagca gcaatgattt caggacggcg cacagatttc
    40981 agaaaatcca gctcttcgcg taatttttca gcgccgcgta aggtcatcgg aatagcttgc
    41041 atttgttata cctcttgaat attcctgatg gggcaagtgt tcacccaccc cggctgttcg
    41101 gcctgcccgg cataactcct caccgggacc agaagcaaaa aaataccgac ccgggtacaa
    41161 gtcccaggtc agctacaatt cacattttga tagtcatttt accctgaagt tcccgaaggg
    41221 tcatcgttta ctttataggg cgttgcgccg tagtatgacg gctcgattcc aggttgttag
    41281 cgcgagatta tgcgattttc cagatttatc atcggattga ccagctgtat agcgttcagt
    41341 gttcaggccg caaatgttga tgagtacatt actcaactcc ccgctggtgc caaccttgcc
    41401 ctgatggtgc aaaaagtcgg cgcgtcggcc cccgctattg attaccacag tcagcagatg
    41461 gcgctgcccg ccagtaccca gaaagtgatt actgcgctgg cggcgttgat tcaactcggc
    41521 cccgattttc gtttcaccac gacgcttgaa accaaaggca atgtggaaaa cggcgtactt
    41581 aagggtgact tagtggcgcg atttggtgcc gatccgacgt taaaacgtca agatattcgc
    41641 aatatggtcg cgactttgaa aaaatctggc gtcaaccaaa tcgatggcaa tgtgttgata
    41701 gatacctcca ttttcgccag ccacgataaa gcccccggct ggccatggaa tgacatgaca
    41761 caatgcttta gcgctccgcc tgccgtcgcc atagttgacc gcaactgttt ctccgtctcg
    41821 ctctacagtg ccccaaaacc tggtgatatg gcttttatac gcgtggcatc ttattacccc
    41881 gttacgatgt tcagccaggt acgcaccctc ccccgtggtt ctgccgaagc gcaatactgc
    41941 gaactggatg tggtgccagg cgacctgaac cgctttacgc tgacgggatg cctgccacaa
    42001 cgttctgagc cgctcccgtt ggcttttgcc gtgcaggatg gagccagcta tgccggtgca
    42061 attctgaaag atgagttaaa acaggcgggt atcacctgga gcggaacact gctgcgccag
    42121 actcaggtta acgaacctgg aacggtagtt gccagtaaac agtcggcccc gctgcacgat
    42181 ctgcttaaga ttatgctgaa aaagtcggac aacatgatcg ccgatacggt tttccgcatg
    42241 ataggccatg cgcgcttcaa tgtgcctgga acatggcggg ccgggtcgga cgctgtgcgt
    42301 cagatcctgc gccagcaagc cggtgtcgat attggaaaca ccattattgc cgatggttca
    42361 gggctttcgc ggcataacct gattgccccc gccaccatga tgcaggtgct gcaatacatt
    42421 gcccaacacg acaatgaact taactttatc tccatgctgc cgctagcggg ctatgacggc
    42481 tctttgcagt accgtgcagg tctgcatcag gcgggcgtgg atggaaaagt ctcagcgaaa
    42541 accggttcgt tgcagggggt atataacctg gcgggattca ttaccacagc gagcgggcaa
    42601 cgaatggcgt ttgtacaata tctttctggc tatgcagtag aaccggcgga tcagcgtaat
    42661 cgccgtattc cgttagtgcg ttttgaaagc cgtttgtata aagatattta tcagaacaat
    42721 tagtcaaaaa gaaaccccgg cacatggccg gggttttgct gattaacgct tgtaaatgaa
    42781 ctcaacgcct tcttcgtcgt cttcgtccca gtcgtcatcc cagtcctcat catcctcttc
    42841 agcaatctct tcaagctgct ggcgatgata atcatcccac atgaattcga ctttctctgg
    42901 ctgtttcgct tcttcagcct gcacgaccgg gttttcaatg ataaaggtca tcacatccca
    42961 gcagagatct ttcacgccca gtccactcgc cgcagagatc agataatatt tatcttccca
    43021 gcccagcgcc tctgcgatcg ctttcgcttt ctcttcggct tctaccttat ccagcagatc
    43081 gatcttgttg aagactaacc aacgcggttt cgccgccaga tcctggctgt atttttccag
    43141 ctcgctgata ataatacgcg cgttttcaac cggatcggtg ccgtcaatcg gatcgatatc
    43201 gatgaggtgc aacaggacgc ggcaacgttc caagtgcttc aggaagcgaa tgcccagacc
    43261 tgcgccttcc gcagcgcctt caatcagtcc tggaatatcg gcaacaacga agctcttttc
    43321 gttgtccatt cgtaccacac ccagacttgg caccagagtg gtaaacggat aatccgccac
    43381 tttcggttta gccgccgata ccgcacgaat aaaggtcgat ttacccgcgt ttggcatccc
    43441 caacataccg acgtcagcca gcagcatcag ctccagcagc agctcacgct tatcgcccgg
    43501 cgtaccgtta gttttctgcc gtggtgtacg gttaactgac gatttgaaac gggtattgcc
    43561 cagaccgtgc cagccgccct tagcaaccag cagacgctga ccgtgtttgg tcatatcgcc
    43621 catggtttca ccagtaccct ggtcgattac acgcgtacct accggcactt taattgtgac
    43681 gtctttaccg cgcttaccgg tacagtcgcg gcttgcgcca ttctgaccgc gctctgcacg
    43741 gaaagatttt tcaaaacgat aatcgataag cgtgttcagg ttctcgtcgg cttccatcca
    43801 tacgtcacca ccatcaccgc cgtcgccgcc atccgggccg cctttcggaa tatacttttc
    43861 gcggcggaag ctcacgcaac cattaccgcc atcacctgca acgaccagaa tcgatgcttc
    43921 atcaacaaac ttcattttat tctccgtaaa tcattcgcct gcgcggggtt gcgataccac
    43981 cgttgtatgc ttacgtaatc cgccccaaat acgatgacca atggcggaat acatcgcgcc
    44041 cgcaaccacg acaaacgcac cgagataacc taaaaggttt aacatcggtc tggcgaagaa
    44101 atcgggccag gccagtgata aaagatctga aaaaaacagc gtaaacagtg gggtgagcgt
    44161 gatgatcgcg ctcacctgcg ctgcctgcca gcgagccatc gcttccgcca gggcgccata
    44221 tcctaccaag gtattcagtc cgcaaaaaat taaacatgcg agctgccagt ggctaagctg
    44281 cgctatcact ccaggctttg ccagagggaa gagcgcaatt gtacataaag tgtacagtaa
    44341 aaacaggatc tgcggtgagg ccagccgacg caataaaacc ttttgcgcca cgccataact
    44401 cacccaaacc gtcgcagcac cgaccccaaa gataactccc caggtgtaat cggttagctt
    44461 tgtaaatatc tcgaccagac tggtgttaaa aaacatcacc aggccgctca ggagcatcaa
    44521 tgcccctaca acctgagtgc tgcgcatttt ctctttcagg ataaatacgc tggcaaccat
    44581 catgccaact ggcgagagtt gcccaatcac ctgcgaagcg gtcggactca ggtattgcaa
    44641 ggatgagctg aacaggatga agttcccaaa cagcccggcg gtcgccactg ccaacaaaat
    44701 caaccagcgt ggcttacgaa acacgcgtaa tggcggcaac ctcttcttca ccgcaagaat
    44761 ggcccccagg ccaatactcg ccatcaagaa acggtaaaac acgattgtcg gaggttccat
    44821 cacctccagc acctgcttca ttgcaattgg caacgccccc cagcaaattg ctgtggtgag
    44881 cgccaaaaga atgccaatgc ctgcctgctg cttcataccg tattccctgc aaagcgcatt
    44941 tgcggattaa cgtcgcacca tgcgcatttt taccggttat cgaatgtaaa aagccccgca
    45001 acgtgttgcg gggctttcat ccgttaccgg gacgcgaaaa acttattcag cttcgatgct
    45061 gataaattta cggtttttcg ggcctttaac ttcgaatttc actttaccgt ctgctttagc
    45121 aaacagagtg tggtcacgac cgcaacctac gttagcgcca gcgtggaatt tggtaccacg
    45181 ttgacgaacg atgatgctac ccgccagaac ggattcgcca ccgaaacgct taacgcccag
    45241 gcgtttagct tctgaatcgc gaccgttacg tgtggagccg ccagcctttt tatgtgccat
    45301 ttgaaatctc tcctcaggtc ttaggcgctg atgccagtaa ttttcacatc agtgaaccac
    45361 tgacgatggc cctgctgctt acgatagtgt ttacgacgac gaaacttaac gattttaact
    45421 ttctcgccac gaccgtgagc aacaacttca gctttgatta cgccgccatc aacgaaagga
    45481 acgccgattt tgacttcttc accgtttgcg atcatcagca cttcagcgaa ctcaacagtt
    45541 tcgccagttg cgatgtccag cttttccagg cgaacggtct gaccttcgct tactcggtgt
    45601 tgtttaccac cactttggaa aaccgcgtac atataaaact ccgcttccgc gcacaccttt
    45661 tcaatgattc agagtgcgct ataaatattc acaatagggc gcgaatatta cgcaaaacgc
    45721 acgcctttga caagtgctac agtcaataca cgaagaaaaa aaacacaact tgtacggtaa
    45781 cgtttatctg tgccattttt tcagtacaat caccctatat tcctaaccat aaaccctaag
    45841 ttgcctttgt tcacagtaag gtaatcgggg cgaaaagccc ggcttttgcg atgaatttag
    45901 aaaaaatcaa tgagttaacc gcgcaagata tggcgggtgt taatgcggca atccttgagc
    45961 agcttaattc cgacgtccaa ctgatcaatc agttaggcta ttacatcgtc agcggcggcg
    46021 gtaaacgtat tcgtccgatg attgctgtac tggctgcacg agctgttggc tatgagggaa
    46081 atgcgcatgt caccattgct gccctgatcg agtttatcca cacggcgact ctgctgcacg
    46141 acgacgttgt ggatgaatca gatatgcgca ggggtaaagc taccgccaac gccgcatttg
    46201 gcaatgccgc cagcgtgctg gtaggcgatt ttatttatac ccgcgcgttc cagatgatga
    46261 ccagcctcgg ttcactcaaa gtgctggaag tcatgtcaga agccgtaaac gtcatcgcag
    46321 aaggtgaagt tctgcaactg atgaatgtta acgatccgga catcactgaa gaaaactaca
    46381 tgcgcgttat ctatagcaaa accgcgcgtc tgtttgaggc tgccgcgcag tgttccggaa
    46441 ttctggcggg ctgtacgccg gaggaggaga aaggcctgca ggattatggg cgctatctcg
    46501 gcactgcttt ccagttgatc gacgatttac tcgattacaa tgccgatggc gaacagttag
    46561 gtaaaaacgt cggcgacgat ctgaacgaag gtaaaccgac gctgccgctg ctgcatgcga
    46621 tgcatcatgg cacaccagaa caggcacaga tgatccgtac cgccatcgaa cagggtaacg
    46681 gtcgccatct tctgaaaccg gttctggaag caatgaacgc ttgtggatct cttgaatgga
    46741 cgcgtcagcg cgccgaggaa gaagcagaca aagccatcgc agcgttacag gtgctcccgg
    46801 acaccccttg gcgagaagca ctcatcggcc tcgcgcacat cgctgttcaa cgcgatcgtt
    46861 aatcccctcc cctcatcccg cgcagtgcgc gggatgattc cagtaaactc cataagaata
    46921 cctattcagc gtaataatgc actgaaaaac cagtgcatac atctttatat attctgaata
    46981 ttcacactct ttataggaac tttttagagc aatcggccat caggagtata gtgatgctcg
    47041 acagaagaag tgttctgaat gaaagcgact aacttaagga gtgaggaaag atggaaagta
    47101 atttcattga ctggcatccc gctgacatca ttgcgggttt gcgcaagaag ggaacatcaa
    47161 tggcggcgga gtctcgcaga aatggtttga gttcctcaac gctggcgaat gcattatcgc
    47221 gcccatggcc gaaaggagag atgattattg cgaaagccct gggaactgac ccctgggtta
    47281 tctggccatc acgctaccat gatccgcaga cccatgagtt tatcgacaga acgcagttga
    47341 tgcgtagcta cactaaaccg aaaaaatgag tggtctggcg gtagccccgc gaacggggct
    47401 gccagctctc agacgattat tcgcctttca cacgctcaat atttgcacct aaagcgcgca
    47461 gtttgtcttc aatgcgttcg tatccacgat cgatgtgata aatacgatca accaccgtcg
    47521 tcccttccgc aatacagcca gccagcacca ggcttgctga tgcacgcaga tcggttgcca
    47581 taacctgtgc gccagaaagt ttttcaacac cgtgacaaat aacggtattg ctttcgattt
    47641 cggcgtgcgc gcccatacgg ctcagctctg gcacatgcat aaagcggttt tcaaagaccg
    47701 tttcggtgat gaatccagtc ccttctgcca ccaggttcaa cagcgtgaac tgggcctgca
    47761 tatcggtcgg gaatgccgga tgcggcgcgg tacgtacgtt aacagccttc ggacgtttgc
    47821 catgcatatc caggctaatc cagtcttcgc cgacctcgat gtccgctcca gcgtcacgca
    47881 gtttcgccag cacggcgtcg agagtatctg gctgcgtgtt acggcagata attttgccgc
    47941 gagaaatcgc cgccgccacc aggaaagtac cggtttcgat acgatccggt agcacgcgat
    48001 agacaccgcc gcctaaacgt tccacacctt cgatgacgat acgatcggtg ccctgaccgc
    48061 taattttcgc acccagcgta atcaggaagt tcgcggtatc gacgatttcc ggttcacgcg
    48121 ctgcgttttc aataatcgtg gtgccttccg ccagggttgc agcacacatg atggtcaccg
    48181 ttgcgccaac gctgacttta tccatcacga tatgtgcgcc tttcagacga ccatcgacgg
    48241 aagctttaac gtaaccttct tccagtttga tggtcgcgcc taattgttcg aggccagaaa
    48301 tgtgtagatc aaccggacgc gcaccgatcg tacaaccgcc aggtagtgaa acttgcccct
    48361 gaccaaagcg cgctaccagc ggccccagtg cccagataga agcacgcatg gttttaacca
    48421 gatcgtaagg tgcgcagaat acattaacgt cgcgggcatc aatatgcaca gaaccattac
    48481 gttctacttt cgcacccagc tggcttagca gcttcattga tgtatcgacg tctttcagtt
    48541 tcgggacgtt ctggatctct accggttctt ccgccagtag agcggcaaaa aggataggca
    48601 gagcagcatt tttagcgccg gaaattgtga cttcgccctg gagcttcgtt ggcccctgaa
    48661 cacgaaattt atccatttag tttgttctca gttaacaatt catatccgct accggcgaat
    48721 cgcccatagc tcaaaagccg ttcagtttgc gatcgcgcgc ccactccgca ggggtatacg
    48781 ctttgatcga cacagcatga atgcggttat ccgcaatata ttccatcagc ggaccataga
    48841 ccgtctgctg ttttttaacc cgactcatgc cgtcaaacaa ctcacccacg gcaataacct
    48901 gaaagtggct gccatcgccg gaaacgtgga cttcctggag ggagagagcg ttcatcaaca
    48961 cgctctgaat ttcattattt tccatgggat cttcaatcat cagttaataa accagcgaaa
    49021 catcttagag caaagttgcg ctggcataaa taagcaaaaa gcctcgctga taaatcagac
    49081 aaggctcgac ttgcaggcag gtttgccgga caggcggtta acgccatatc cggcctgaaa
    49141 aaatttaacg aggcagaaca tcagcaggca aattatacaa tttcgccagg gtatacactt
    49201 tgtcgtttac cccctgaagc gtcacgttgt tgccctgctt tttcgccaga tcgataagat
    49261 ggagcagcag tgccagtcca cccgtatcca cgcgggacac acggctaaga tcgatgcagg
    49321 taatcccctt caccgcttcc tcacgcattt cccaaagcgg tagcagaacg tcctgatcca
    49381 gctctccgga taacgccagt gtgtcacccg tctgcatcca gctcagtgac tcgctcatta
    49441 ttttttctct tccagagtga ttttctgttg agaaatcgat ttcagttgcg cagtcaggcc
    49501 gtcgatacct ttggtacgta gcagcgttcc ccactcgttt tgtttggtgg tgatcatact
    49561 gacgccttca gcaatcatgt cgtaagcctg ccagttgccc gtctgggagt ttttacgcca
    49621 ctggaagtcc agacgcaccg gcgggcggcc attcgggtca ataatggtaa cgcgaatagg
    49681 cacaatggtt ttatcaccca gcggctgttc tggcgcaatc tgataggttt gaccgtgata
    49741 catcgccagc gcctgaccgt aagcctgctt caggtactca cggaaagcgg caaagtaagc
    49801 atcacgttga gctggggtcg cactcttgta atactggccc agcaccagcg caccggcgta
    49861 tttcacctgt acgtatggca gcagttcctg atcaacaatg gtacgcagat aatccgggtt
    49921 ggcccgaatt tgcggttgct cattcttcag gcgatcgaac gttttctgcg ccgcctcgtc
    49981 catcagctta tacggattgg tctggtctgc cgcggttgcc gcactcagcg gtgcaatcac
    50041 cagcaaagcg accatcatta aacgtttaaa catgcgtcgg ttctcctgaa attatttcgt
    50101 tgtacccaca ggttcagtgg tttcattatt acctggcgca gcagctggcg catcgccact
    50161 attcttattg tcatcgcctt tactaccgta aaggaactga ccaatgagat cttccagcac
    50221 catcgcagac ttggtgtcct gaattgtatc gccatccttc aggatagcag tccccagttc
    50281 cgggtcttca aaaccgacgt ttaatgccag atattgttcc cccagcaggc cggaagtacg
    50341 aatgctcagc gaactggtat caggaatatg gttataacgt tgttcaattt ccagcgttac
    50401 gcgcggcaga taggttttcg ggtccagcgt aatatccgcc acccggccca caacaacgcc
    50461 accaatactg accggagagc gcgctttcag gccgccaatg ttatcgaacg tcgcataaag
    50521 cgtgtaggtc ggttcagtac gtattgacgt cacgttcgcc gccttcaggc aaacaaacag
    50581 cgccgccagc agtgctgcta ataaaaagat acccacccaa atttcatttt ttttcgtttg
    50641 catgaactca attcccaaac atcaatgcgg tcagcacaaa atccagcccc agaacagcca
    50701 gagacgagtg gacaacggtg cgagtggttg cccggctaat cccggcagac gtcgggatgg
    50761 cgtcgtagcc gttaaacaac gaaatccacg tcaccgtgat ggcgaacacc acgctcttaa
    50821 tcagacagtt gaccagatcc atacgccagt cgacggcatt ttgcattgcc gaccagaaga
    50881 acccgctatc aatgcctttc caactgacgc cgaccagcga tccgccccag atccccacgg
    50941 cgacgaagat aaccgtcaac agtggtaatg aaataacccc agcccagaaa cggggagaaa
    51001 taacccgacg cagcggatcc accgccatca tctccatact ggagagttgc tctgtagcgc
    51061 gcatcaggcc gatttctgcg gttagcgccg aaccagcacg cccggcaaac aacaacgcgg
    51121 caaccaccgg ccccagttca cgcagtagcg ataacgccac cagcataccc agactggttt
    51181 ccgcactata agtggtcaga accagataac cttgcagccc caacaccatt ccgatgaaca
    51241 cgccagaaac tacaataatc agcatcgaca ggacgccgac attatagagc tggcgcacca
    51301 gcagcggcgc atgtttgcga aattccggtt tgccgaccag cgcattgaat aacattaacc
    51361 cggcccgccc aaacgttctc agggttttaa tccctttatg tccgagcgac gccagcgcat
    51421 ttaacagcat gagtggctta actccctggt aaaagatcag cgtgataatc gccggcagga
    51481 tagcggaacg gaaccggccc gtcagctatc ccgtccagaa actgacgtac gcgcggatca
    51541 ggattcgcct gcaacgcctg ggcactgcca tgagcgacaa tttttttgtc cgccaggatc
    51601 caggcgtgat ccgcaatact taacacttcc ggcacatcgt gagaaaccac cacacaagtc
    51661 acgcccagcg cgctgttcag ctcagaaatc agcttcacca gtacgcccat ggtgatggga
    51721 tcttgcccaa caaaaggttc atcaaacatg atgagatccg gctccagcgc aatcgcacgc
    51781 gccagcgctg cacgccgcgc catcccaccg gaaagttcag aaggcattag tttagccgct
    51841 ccacgcagcc ccacggcctc cagcttcatc atcaccgtac tatgcaacaa tggcgcggga
    51901 agttgggtat gttcgcgcag tggataggcg acgttgtcaa atacgttcat atcagtgaac
    51961 aacgccccgg actgaaataa catgctcatc cgtttgcgca ctgtatacag gcgcgaacga
    52021 gacatcgccg gaatattctc accatcgaaa aggatctcac catgatctgg tgcgatttgc
    52081 ccgccaatca gacggagtag cgtcgtttta ccgatgcccg atggccccat gatcgccgtg
    52141 atcttccctc gcggcacggt cagggaaata ttatcgaaga tgcagcgatt gccacgcgta
    52201 aaactgacat cgcgcatatc gactaaattc gccacagact gctccataat tcacccttcg
    52261 tcttgcgttg attttctaag catggcgctc aatttaacct tgaacccaac atatttacag
    52321 aatattaccc gccgtggtta gcgaaagctg gcatttgttt tactttttag ccgcataaag
    52381 tcaaaattaa gaatccgtta cggctttctg aaaatcttca gcggaccggc gagtatacct
    52441 gaagaaagga cgttagatgc ttttagctac ggcactgtta attgttggtt tacttttggt
    52501 cgtttacagt gccgaccgcc tggtttttgc cgcgtctatt ctttgccgaa cctttggcat
    52561 cccgccgctg atcatcggca tgacggtggt cagtattggt acatcgttac cagaaatcat
    52621 cgtctcgctt gccgcgtctc tgcacgaaca acgcgattta gccgttggta cagccctcgg
    52681 ctcaaacatt atcaatatat tgctgatcct cggtctggct gcgctggttc gtccttttac
    52741 cgtccattcc gatgttctac gccgtgaatt acccttaatg ttgttggtca gcgtggtggc
    52801 cggttccgta ctctatgacg gacaacttag tcgcagcgat ggtatctttc tcctctttct
    52861 ggctgtgcta tggctgctgt tcattgttaa acttgcacgt caggctgaac gtcaggggac
    52921 tgacagcctg accagagagc agcttgcaga gctgccgcgt gacggcggat tgcccgtcgc
    52981 gtttttatgg ctcggcattg cgcttatcat catgccagtg gccacgcgga tggtggttga
    53041 taacgccacg gtgctggcga attactttgc catcagcgag ttgacgatgg gtctgacggc
    53101 aattgctatc ggaaccagcc tgccggaact ggcaaccgca atagcgggcg ttcgcaaagg
    53161 tgaaaacgac attgctgtcg gaaatatcat tggcgcaaac atttttaata ttgtcatcgt
    53221 gttgggttta cccgcgctga taacgccagg agagattgat ccactggcgt acagtcgtga
    53281 ctacagcgtg atgttgctgg tgagcattat ttttgcgttg ctgtgctggc ggcgctcccc
    53341 gcaaccgggc cgtggtgtag gggtattatt aactggcgga tttatcgtat ggctggcgat
    53401 gttgtactgg ttatcgccaa tactcgttga ataactggaa acgcattatg tcgcacgtag
    53461 agttacaacc gggttttgac tttcagcaag caggtaaaga agtcctggcg attgaacgtg
    53521 aatgcctggc ggagcttgat caatacatca atcagaattt cacgcttgcc tgtgaaaaga
    53581 tgttctggtg taaagggaaa gttgtcgtca tggggatggg gaaatcgggg cacatcgggc
    53641 gcaaaatggc cgcaacgttt gccagcaccg gtacaccttc atttttcgtc catcctggtg
    53701 aagccgcgca tggtgattta ggcatggtca ccccacagga tgtggtgatt gctatctcta
    53761 actcaggtga atccagcgaa atcactgcct taattccagt gcttaagcgt cttcacatac
    53821 cgttaatctg catcaccggt cgcccggaga gcagcatggc gcgcgccgca gatgtgcatc
    53881 tgtgtgttaa agtagcgaaa gaagcctgtc cgttagggct ggcaccgacc agcagcacca
    53941 ccgccacgct ggttatgggc gatgccctcg ctgtcgcgct gttaaaagca cgcggcttta
    54001 ctgctgaaga ttttgcgctc tcacacccag gcggcgcact gggtcgtaaa cttctgctgc
    54061 gcgtaaacga tattatgcat acgggcgatg agatcccgca tgttaagaaa acggccagtc
    54121 tgcgtgacgc attgctggaa gttacccgca aaaatcttgg tatgactgtc atttgcgatg
    54181 acaatatgat gattgaaggc atctttaccg acggtgattt acgccgtgtc ttcgatatgg
    54241 gcgtggatgt tcgtcagtta agtattgccg atgtgatgac gccgggggga atacgcgtgc
    54301 gccctggcat tctggccgtt gaggcactga acttaatgca gtcccgccat atcacctccg
    54361 tgatggttgc cgatggcgac catttactcg gtgtgttaca tatgcatgat ttactgcgtg
    54421 caggcgtagt gtaaagattc aaggataaac aacaatgagc aaagcaggtg cgtcgcttgc
    54481 gacctgttac ggccctgtca gcgccgacgt tatagcaaaa gcagagaaca ttcgtctgct
    54541 aatcctcgac gttgatggcg tactgtcaga tggcctgatt tatatgggca ataacggcga
    54601 agagctgaaa gcgttcaatg ttcgtgacgg ttatggcatt cgttgtgcgc tcacctctga
    54661 tattgaagtc gctatcatta ccgggcgaaa ggctaaactg gtagaagatc gttgtgccac
    54721 attggggatc actcacttgt atcaggggca gtcaaacaaa ctgatcgcct ttagcgatct
    54781 gctggaaaaa ttggcgattg ccccggaaaa tgtggcttat gtcggcgatg atctcatcga
    54841 ttggccggta atggaaaaag tgggtttaag cgtcgccgtg gccgatgcgc atccactgtt
    54901 gatcccgcgc gccgattacg tgacgcgcat tgctggcggt cgtggcgcag tgcgagaagt
    54961 ttgcgactta ttactcctgg cgcagggcaa actggatgaa gccaaagggc aatcgatatg
    55021 agtaaagcca gacgttgggt tatcattgtg ctatcactgg cggttctggt gatgatcggt
    55081 attaatatgg ccgaaaaaga cgataccgcc caggtggtcg tcaacaacaa tgatcccacc
    55141 tataaaagcg agcatacgga cacgctcgtc tataacccag aaggggcgct aagctatcga
    55201 ttgattgctc aacacgttga atattattcc gatcaggccg tttcgtggtt tacgcagccg
    55261 gtacttacca cgtttgataa ggataaaatc ccgacatggt ccgtaaaagc agataaagcc
    55321 aagctgacca atgaccggat gctctattta tatggacacg ttgaagtcaa cgcacttgtg
    55381 ccggactctc aacttcgcag aatcacgacg gataacgcgc agatcaatct ggtgacgcag
    55441 gatgttacct ctgaagacct cgtcacgtta tacggaacaa catttaactc cagcggtctg
    55501 aaaatgcgcg gcaacttacg cagcaagaac gccgagctga ttgaaaaggt tagaacatcc
    55561 tatgaaattc aaaacaaaca aactcagcct taatcttgtg cttgccagct cacttctggc
    55621 cgccagcatt ccggcatttg ccgtaaccgg agacactgat cagccgatac acattgaatc
    55681 ggaccagcaa tctcttgata tgcaaggcaa cgtggttacc tttaccggta atgtcatcgt
    55741 cacccagggc accataaaaa ttaatgccga caaagtggtc gttacccgtc cgggtggcga
    55801 acaaggtaaa gaagtgattg acggctacgg taaaccggca acgttctacc agatgcagga
    55861 caacggtaaa cccgttgaag gtcacgcttc ccagatgcac tacgaactgg caaaagattt
    55921 tgtcgttctg acgggtaatg cttatctgca gcaggtcgat agcaacatta agggcgataa
    55981 gatcacttac ctggtgaaag agcagaaaat gcaggctttc agcgacaaag gcaagcgtgt
    56041 aaccaccgtt ctggtgccgt cgcagctgca ggacaaaaac aacaaaggcc agaccccggc
    56101 acagaagaag ggtaattaat tcgttatggc aacattaact gcaaagaacc ttgcaaaagc
    56161 ctataaaggc cgtcgcgtgg tagaagacgt cagcctgacc gtcaactccg gggaaattgt
    56221 cggtctgctg gggccaaacg gtgccggtaa gaccaccact ttctacatgg ttgtaggcat
    56281 tgtgccgcgc gatgcgggca acatcattat tgatgatgac gatatcagtc tgctacctct
    56341 gcatgcacgc gcgcgccgcg gtatcggcta tctgccacag gaagcctcca ttttccgtcg
    56401 cctcagcgtt tacgataacc tgatggcggt actgcaaatt cgtgacgact tgtctgctga
    56461 acagcgtgaa gaccgcgcga acgagctgat ggaagagttt cacattgagc acctgcgtga
    56521 cagcatgggg cagtcactct ccgggggtga acgtcgccgt gtagaaattg cccgcgcact
    56581 ggctgcgaat ccgaaattta ttctgctcga cgaaccgttt gccggggttg acccgatctc
    56641 agttatcgac attaaacgca tcattgagca cctgcgcgac agcggcctgg gcgtgctgat
    56701 cactgaccac aacgtgcgtg aaacactggc ggtttgtgaa cgcgcttata tcgtcagtca
    56761 ggggcatttg atcgcccacg gcacgcctac agaaatctta caagacgaac acgttaagcg
    56821 tgtatacctt ggggaagact tcagactctg atagggtaga agtttgcgac gttttagcag
    56881 gagagtacga ttctgaacat gaagcaaggt ttgcaactca ggcttagcca acaactggcg
    56941 atgacgccac agctccaaca ggcaattcgt ctgttgcagt tgtcgacgct ggaacttcag
    57001 caggagctac agcaggcgct ggagagtaat ccgctgcttg agcaaatcga cactcatgaa
    57061 gaaatcgaca cccgcgaaac gcaagacagt gaaacgctgg acaccgccga cgcgctcgaa
    57121 caaaaagaga tgccggaaga gctgccgctc gatgccagtt gggacaccat ttacaccgct
    57181 ggtacaccat ccggcaccag cggtgactac attgacgacg agctgccggt ctatcagggc
    57241 gaaacgacgc agaccttgca ggattacctg atgtggcagg tcgagctgac accgttttcc
    57301 gacactgacc gcgctattgc tacctctatc gtcgatgccg ttgatgacac cggttatctg
    57361 actgtcccgc tggaagatat tctcgaaagt atgggcgatg aagagatcga catcgacgaa
    57421 gttgaagccg tccttaagcg gatccaacgg tttgatccgg tcggtgtggc ggcaaaagat
    57481 ctgcgtgact gcctgctgat ccaactctcc caattcgata aaaccacgcc gtggctggaa
    57541 gaggccagac tgatcattag cgatcatctc gatctgttag ccaatcacga cttccgcact
    57601 ttaatgcgcg tcacgcgtct gaaagaagat gtgctgaaag aagccgtcaa tctgatccag
    57661 tcgctcgatc cgcgccccgg gcaatcgatc cagactggcg aacctgagta tgtcattcca
    57721 gatgtgctgg tgcgtaagca taacggtcac tggacggtag aactcaacag tgacagcatt
    57781 cctcgtctgc aaatcaacca gcactacgcc tcgatgtgca ataacgcgcg caatgatggt
    57841 gacagccagt ttatccgcag caatctgcaa gatgccaaat ggttgatcaa gagtctggaa
    57901 agccgtaacg atacgctact gcgcgtgagt cgctgtatcg ttgaacagca gcaagccttc
    57961 tttgagcaag gcgaagaata tatgaaaccg atggtactgg ccgatatcgc ccaggctgtc
    58021 gaaatgcatg aatcgacgat atctcgcgtg accacgcaaa aatacctgca tagtccacga
    58081 ggcatttttg aactgaagta tttcttttcc agtcacgtca ataccgaggg cggcggcgaa
    58141 gcttcctcca cggcgattcg tgcgctggtg aagaaattaa tcgcggcgga aaacccagcg
    58201 aaaccgttga gcgacagcaa gttaacctct ttgctgtcgg aacaaggtat catggtggca
    58261 cgccgcactg ttgcgaagta ccgagagtct ttatccattc cgccgtcaaa ccagcgtaaa
    58321 cagctcgttt gacccaaccg ataaggaaga cactatgcag ctcaacatta ccggaaataa
    58381 cgtcgagatc accgaggcac tgcgcgaatt tgttacagcc aaatttgcca aacttgagca
    58441 atattttgac cgaatcaacc aggtctatgt tgttctgaaa gtggagaaag tcacccacac
    58501 ctcagatgca acactgcatg taaacggtgg tgaaattcat gccagcgcgg aaggtcagga
    58561 catgtacgct gccattgatg gcttaattga taagctggca cgccagttga ccaaacataa
    58621 agataaactg aaacaacact aattgtccgg gcaattagca tgtgcatggc ggcctgttgt
    58681 gcggcacaac gggccatttg tacggttaat gctccgagcc tgttccactg tttgagtggg
    58741 caggttctta ggtgaaatta tgacaaataa tgatacaact ctacagctta gcagtgttct
    58801 taacagggaa tgtacgcgaa gccgcgtcca ctgtcagagc aaaaaacgcg ccctggaaat
    58861 catcagcgag ctggcggcga aacaacttag cctaccgcct caggtggttt ttgaagctat
    58921 cctgacgcgg gaaaaaatgg gcagtaccgg tatcggcaat ggtattgcca ttccgcatgg
    58981 caaactggaa gaagatactc tgcgtgccgt tggcgttttc gttcagctcg aaacgcctat
    59041 agctttcgat gccatcgaca accaaccggt ggacttactt tttgccctgc tggtgccggc
    59101 agaccaaact aaaacgcacc tgcatactct gtcgctggtg gcgaaacgtc tggcggacaa
    59161 aaccatctgc cgccgtttac gcgcagccca gagcgatgaa gagctgtatc aaatcattac
    59221 ggataccaaa ggtactccgg atgaagcgta gttattcggt aatgtctctt ttagacgttg
    59281 tgaggagaaa cagtacatgg tactgatgat cgtcagcgga cgttcaggtt caggtaaatc
    59341 tgtcgccctg cgtgcgctgg aagatatggg tttttactgc gtggataacc ttcccgtagt
    59401 gttgttaccc gatctggctc gaaccctggc cgatcgcgag atttctgccg ccgtcagcat
    59461 tgatgttcgt aatatgccgg agtcaccaga aatattcgaa caggcgatga gtaacctgcc
    59521 tgacgctttc tcaccgcaac tactgttcct ggatgccgac cgtaatacct taattcgtcg
    59581 ttacagtgac acgcgccgac tgcatccgct ttccagcaaa aacctgtcgc tggaaagtgc
    59641 tatcgacaaa gaaagcgatc tgctggagcc tctgcgttcg cgagcggatc tgattgtcga
    59701 tacctcagaa atgtccgttc acgagctggc agaaatgctg cgtacccgtc tgctgggtaa
    59761 acgtgaacgc gaactgacaa tggtctttga gtctttcggc ttcaaacacg gtatccctat
    59821 cgatgcagat tacgtctttg acgtgcgctt cttgccgaac ccgcactggg atccgaaact
    59881 gcgtccaatg acaggtcttg ataagcctgt cgccgcgttc ctcgaccgcc acacagaagt
    59941 acacaatttt atctaccaga cgcgaagcta tcttgagcta tggttaccta tgctggaaac
    60001 caacaaccgt agctacttga cggtcgccat tggttgtacc ggcgggaagc accgttcggt
    60061 gtatattgca gagcaactgg cagactactt ccgctcgcgc ggtaaaaacg tccagtcacg
    60121 ccatcgtacg ctggaaaaac gtaaaccatg actgtcaagc aaactgttga aatcacaaac
    60181 aagctgggca tgcatgcccg gcctgcaatg aagctgtttg aattaatgca gggttttgac
    60241 gctgaagtgc tgttacgtaa tgacgaaggc accgaggctg aagccaacag cgttattgcg
    60301 ctgctgatgt tggattctgc caaaggacgg cagattgaag ttgaagcgac cggtccacag
    60361 gaagaggaag cactggccgc cgttatcgcc ctctttaatt ctggttttga tgaagattaa
    60421 tcttcatcac tttgacatac aactcccttc aaactccccc cgacaataag aaaatcacgt
    60481 actgaaatcg ttctcaatca acgtcaattg tacatttcgt gcgcttttta ctttcagaag
    60541 aaccttaaga aaaccttaag aagcattgtt taggtttcgt ttaagttaat cgaccatact
    60601 ggagatcgtc agaaaatatt tccaggagat ggcatgattc gcttatctga acaaagtccc
    60661 ctgggcaccg gaagacatcg gaaatgttat gcgcatccgg aagatgccca acgctgtatc
    60721 aagattgtct accatcgtgg cgatggtggc gataaagaga tccgtcgtga gttaaagtac
    60781 tacgcgcatc ttggtcgccg gttaaaagac tggagtggaa taccgcgcta tcacggtacc
    60841 gtcgagacgg attgcggaac cggttatgtt tatgatgtga ttgctgattt tgacggcaag
    60901 ccctccatta cgctgaccga atttgctgaa caatgccgtt acgaagaaga cgttgcacaa
    60961 ctgcgccaat tactgaaaca gctgaagcgt tatttgcagg ataaccgtat cgtgacgatg
    61021 tcgctgaagc cgcagaatat cctctgtcac cgcattagtg agtcagaggt tatccccgtc
    61081 gtctgcgaca acattggtga aagcacattg attccactgg cgacctggtc aaagtggtgt
    61141 tgtctgcgta agcaagagcg gttgtggaaa cgatttatcg cccaaccggc gctggctatt
    61201 gccctgcaga aagatctgca acctcgtgag agcaaaacgc tggccctcgc ttcgcgcgaa
    61261 gcttaatcca gctggtgttg ttgcataaac ggctcaccgc ctaactgata catctgccgt
    61321 aaaatccacg cctgacggct gcgcacgtag cccgatggcg cggagacttt gaaacgaagt
    61381 ggattaggta atacagctgc cagtaaggca gcttccgatc gggtaagttt gctcgcgggt
    61441 ttgtggaaat aacgttgtgc cgcagcttcg acgccaaaca caccgtcgcc aaattcggcg
    61501 atattcaggt aaaccgtcag gatacgcttt ttgctccaga cagtttctat ccccagcgtt
    61561 aatccggctt ccagcccttt tcgaacccag ctacgcccat cccataaaaa gagatttttg
    61621 gctgtctgtt gagaaatcgt tgaagcaccg cgaatacggt tttcattgcg ctcattgtgc
    61681 gccagggctt tctcaatgga agcgacatca aagccccagt gctcaggaaa tgtctgatct
    61741 tctgcggcaa tcaccgccag tcccatccac ggcgagattt gatccatact gaccctgtca
    61801 gaatgtgcca cgtaacgaaa attaccatgc agccaggcgc tgacctgtcg ctcgaccatt
    61861 accgcagaga agggaacagg tgcaacgcta aacaacgcga tgcccccgcc ccagaaaacg
    61921 gcgaggacaa ccattaaccg cagaagaaaa cggcgaacga aactaaacac cgttaagcgg
    61981 cttttactca ttcagccaga accagcacgc gggaaaccag cttatcaatg ccgctcgccg
    62041 cttctgcaat gttctgcgcc agcatatatg ccggggtggt gacaatctta ttgtcttcat
    62101 caaccacgat atcatcgaca ggacacggca catgctccgc gcccatctct tccagcactt
    62161 ctgcggtatc gatatcagta ccgatggtca aacgcagcgg gaaatcgaaa attttcggca
    62221 gcatcgccgg ggcaatacac ataaaaccaa gcggttttcc ggcctgatgc atcgcttgtg
    62281 ccagcgcctt taattcacgg tcaacggtgc attcgcgacc aagactggca aaattgctta
    62341 aattctttgc cgcgccaaac ccccccggca caatcaacgc atccagttca gcggcatcgg
    62401 cctgggccag aggacggatt tcaccgcgcg ttattcgtgc cgcttcaatc agcacattgc
    62461 gcgtttccgt catcgcttcg ccagttaaat gattgataac atcaacctgc tgcttatccg
    62521 gtgcaaagca gaccgcctgc gcaccgctgc gtgaaatagc taacaacgtc aacaccgctt
    62581 catgaatttc agaaccgtca tagacgccgc atccgctcag aattacgcca attttcttca
    62641 ttatgatcat cctttcgcaa ttgactgaaa cacattaatt tttttaataa aaatggtacg
    62701 catcacacat ttaactgatt catgtaacaa atcatttaag ttttgctatc ttaactgcgt
    62761 gcggcctgaa aaacagtgca gtgcccttgt aactcatcat aataatttac ggcgcagcca
    62821 agatttccct ggtgttggcg cagtattcgc gcaccccggt ctagccgggg tcatttttta
    62881 gtggcttttg ccacccacgc tttcagcact tctacgtcgt gacgccactc ttctttcatc
    62941 tcttcaatcc attcaccgac gttatcttcc caggcgggaa ggtcaggaga ctgaatttgc
    63001 tgacccagct gttgcagatg gcgcaacccc actgaccccg ccgcaccttt aattttatgt
    63061 ccttcctcaa caatgccttt tttatcctgc gccgtcagat tcgactccag cacgttgaca
    63121 tagcctggca tcatcttctc aaacaccgct aacccgtcgg tgatcagctt cggtcctaca
    63181 agttcgagat actgttccag catgggaata tcgagcaatg cttctgattt actgttctct
    63241 tctgtcgtca ccgtactctc ctcatcatcc tgggtatccc agaatttctt gatcatcgcg
    63301 gttagcgccg gaacagaaag cggcttactc agcacatcat ccattccagc attgaggtac
    63361 tcttgtttgt ctttcagcac gttagcggtt aaggccacca gcggcggtaa atcctctcgc
    63421 ggataacgct tcgtcagttc acgagagata tccagcccgg tcatatctgg caactgaata
    63481 tccagcaaca ccaggtcgta ttcgcccggt ttaaacatct ccagcgccgc cttgccggtc
    63541 atggcgacat caacgctgtt acctaatttt tccagcacag aacgcgcgac aatcacgttc
    63601 agttcaatgt cttccaccag cagcacattc agcgccggta aaggcatatc gtcttcatca
    63661 aacgcatcat cgacctcttc tgctaccgac ggtgcgtgga tcgtcaacgt aaaggttgaa
    63721 cctttaccct gttcgctggt aaccgtaata tcgccgccca tatttttcgc cagacgacga
    63781 gaaacggcca gaccaatacc ggtgccggtg gcaggtttac cgccatgact gtctttcacc
    63841 tggtaataca tggcgaagat tttatccagc tcatcctgcg gaatgccgat accagaatct
    63901 tccacttcaa aatgcagcat atcgccttca tcgtagcgca cgcgcacggt aacctggcct
    63961 tgctgggtga atttgacggc gttactgatg aggttccaca ggatctgccg taaacgcgtc
    64021 ccgtcggtaa tgacctgatg cggtaatggc agcgtcggct ccaggttaaa gcgcaatcct
    64081 ttttgttgcg cctgcaatgc ggagagattt tccagatcgg caaggaagct ggtgaaatca
    64141 accggttgat tatcaagctg gaccttgcgc cgttccatct tatccatgtc gataatatcg
    64201 ttaaagatat tccccagcgt gacggccgaa acatggatgg tcttgagata tttttcctgc
    64261 tcggcggtga gttcggtatc cagcagaatg cggctcaggc cgacgatacc gttcagcggt
    64321 gtacgcaatt cgtggctgat ggtggaaata aacgtcgttt tgtcgcggct ggcgcgttca
    64381 agcgcatcct gataccgctt acgctcggta atgtcgcgac caaagcccat caaaccgtga
    64441 cgtttaccca cgcggtcgta gtacggcact ttacgaattt caaagcaggc tttgcgtccg
    64501 tcgggataat ccagccactg ctcataggtc agtgacacat tatgacggaa cactttttca
    64561 tcggtttcaa tgactttcgc agcggcttcc ggtgagtaaa catcagcagg tttcaggtga
    64621 accagttgtt tttcgctttt tccggtcagc agctccatcg cgcggttaca gccggaaaac
    64681 tctttatctt cgttacgata aaaaaccagg tcgggtgaag catcaaggaa ggaacgtaaa
    64741 aatgaggatt gctgctcgag ctgaatttgt gtctcttcgc gctctttgat ttcaattttc
    64801 agttggccga aggtttcctg cagttctgct tccgcttttt cacgaacggc aatttcctga
    64861 tttagctggg caatattatc ttttaactga acgttgaggc tcaaatcgcg ctcgcgcatc
    64921 tcctccagtt tttgcaccag ccgtgacaga cgttgtcgtg actcctccag ttgctcgacg
    64981 accaccgata gaaagtagac cgcccacggc gtaatcagca aaccaaagaa gatagaacga
    65041 ataacatcaa tgctttcgac ctgaccatgc agcaccatgg ttaccgccat ttgtaccaca
    65101 atggcaagaa cgacgagggc cagcgccagc aacattgaga agcgcaccag acctaacttc
    65161 atcatcaggt caacataata ctgcgccagc agacgaattt gcttcattag ggaattcctt
    65221 cacgacaacc tgccccaata atacccaatt ctgacagtta cgttataaat tgtgtgagaa
    65281 atgcggaatt cccctcaacg acctactccg ttggaggtag ccacggacgt cccagcgccg
    65341 atccctgtac gccatgttca ttcagatagc gatccagctc gaccattccc gtccatcgat
    65401 tttcgcacca cagaggagca agcagcgtcg ggcgacgggc gctggcggaa atgcggtggt
    65461 agatcacttc cggtggcgta tggcgaatca tttctccggc agtgagcgtg taatcctcca
    65521 gttcaatacc gttcaaacgc cccgcttccc aggctttcgc cataatgctg cctttcacaa
    65581 tatgcagcgg atgcagcttt atgccgtcca cgccggtttc aaccactcgc tccagcgttt
    65641 gcaaacattc agcctgccct tcgccaggca ggccgacaat taagtgagaa cacaccttca
    65701 gcccgcgctg acgtgccagc tgggttgtac gctgataaca ggcaaaatca tggccgcgat
    65761 taatgcgatg cagggttttg tcgtgggcgg tttgtagccc cagctccagc cacacttcgt
    65821 agccctggtc cttatattcg caaagcagat ccagcaccgc atccggcacg cagtccgggc
    65881 gagtaccaac acacaaaccg acaatattgg cctggctcac cgcctgctga tacatagaac
    65941 gcagcacctg aacttccgca aaggtgctgg tatacgcctg aaagtaggcc agatagcgtt
    66001 tagcgcggtt aactaaattc gcctggtgcg ccagttgctc ggcaatggaa cgatgttgct
    66061 gcgcttcatc ggcaaatgag gcaacattac agaatgtgca gccgccacgc ccgatggtac
    66121 cgtcacggtt agggcagcta aaaccgccgt gcagcgtcag cttatgcacc ttttgcccat
    66181 aacgacgggt gagatcacca ccaaacatat tgactaattt ctgtaactgc ataatctgat
    66241 agaccgcgcc ttgaaaagag gccaaagcct gccattttta gctcaattcg gcgatgactt
    66301 ggatcaatcg tcccggcctg cttttatcaa ctgcataacc aatcaaaatt accgaaattt
    66361 catgcataat cacataaatc acttttgctt atcttgtgtc agattttttt atctccttat
    66421 ggattttagg caaaacagta gcatgaaacg tcattaccaa ttaaggcagt ataaaatgct
    66481 ggttttctcg tcagttcaag gcaggataag ggttaacacg cctttatgac agtcaggaat
    66541 tggctgtttc tctaacgacg ccccttttag ccttaattag aaaatccatt ttaatttcag
    66601 tcaattaata aagaattttg cgctaaagca catttctgta ccaataagct tgccatttga
    66661 cctgtatcag ctttcccgat aagttggaaa tccgctggaa gctttctgga tgagcagcct
    66721 gctcatcata tttatgcagt aattgagatc ccctcttcac cgtattaacc gatgcgaaaa
    66781 ggacaacaag ggggcgaatg cgaggcgcgc gtatgacacg caaaccccgt cgccacgctc
    66841 tttctgtgcc cgtgcgcagc ggttcggaag tggggttccc gcagagcctg ggggaggttc
    66901 acgatatgtt gtacgataaa tcccttgaga gggataactg tggtttcggc ctgatcgccc
    66961 acatagaagg cgaacctagc cacaaggtag tgcgtactgc aatacacgca ctggcccgca
    67021 tgcagcaccg tggcgcgatt ctcgccgatg gtaaaaccgg tgacggttgc ggcttgctgt
    67081 tacaaaaacc ggatcgcttt tttcgcatcg ttgcgcagga gcgcggctgg cgtttagcga
    67141 aaaactacgc cgtcgggatg ctcttcctga ataaagatcc tgaactcgcc gctgccgcac
    67201 gccgcatcgt tgaagaagag ctgcaacgcg aaaccttgtc gattgtgggc tggcgtgatg
    67261 tccccactaa cgaaggcgtg ctgggtgaaa tcgccctctc ctctctgcca cgcattgagc
    67321 aaatttttgt gaacgccccg gcaggctggc gtccgcgcga tatggagcgc cgtctgttta
    67381 tcgcccgccg ccgcattgaa aagcgtctcg aagccgacaa agacttctac gtctgtagcc
    67441 tgtcgaatct ggtgaacatc tataaaggtc tgtgtatgcc ggcggatctg ccgcgctttt
    67501 atctagatct tgcggacctg cgtctggaat cggccatttg cctgttccac cagcgcttct
    67561 ccactaacac cgtaccgcgc tggccgctgg cgcaaccgtt ccgctacctg gcgcataacg
    67621 gtgaaatcaa caccatcacc ggtaaccgcc aatgggcgcg tgcgcgtact tataaattcc
    67681 agacaccgct tatccctgac ctgcacgacg ccgcaccgtt cgtcaacgaa atcggctctg
    67741 actccagttc gatggataac atgctggaac tgctgctggc aggcgggatg gatatcatcc
    67801 gcgccatgcg tctattagta ccacccgcct ggcagaacaa cccggatatg gacccggaac
    67861 tgcgtgcctt ctttgacttt aactccatgc atatggagcc gtgggatggc ccggcgggca
    67921 tcgtgatgtc tgacggtcgt tttgctgcct gtaacctcga ccgtaacggt ctgcgtccgg
    67981 cgcgctacgt catcaccaaa gataagctca tcacctgcgc ctctgaagtc ggtatctggg
    68041 attaccagcc tgacgaagtg gtcgaaaaag gccgcgtcgg gccaggcgaa ctgatggtaa
    68101 tcgacactcg cagtgggcgt attctgcact cggcagaaac cgatgacgat ctgaaaagcc
    68161 gccaccctta taaagagtgg atggagaaaa acgtccgccg actggtaccg tttgaagatc
    68221 tgcccgatga agaagtgggt agccgcgaac tggacgacga cacgcttgcc agctaccaga
    68281 aacagtttaa ctacagcgcg gaagagctgg actccgtaat tcgcgtactg ggcgaaaacg
    68341 gtcaggaagc ggtcggttcg atgggcgatg ataccccatt cgccgtgctc tccagtcagc
    68401 cgcgcattat ttacgactac ttccgccagc agtttgccca ggtgactaac ccgccaatcg
    68461 acccgctgcg tgaagcgcat gttatgtcgc tcgccaccag tatcggtcgt gaaatgaacg
    68521 tcttttgcga agcagagggc caggcgcacc gtttaagctt taaatcgccg attctgctct
    68581 actccgattt caaacagctc acgacgatga aagaggagca ctaccgcgca gatacgctgg
    68641 atatcacctt tgacgtcacc aaaaccacgc tcgaagcgac ggtcaaagag ctgtgcgaca
    68701 aagccgaaaa aatggtacgt agcggcaccg tgctgctggt gctctccgac cggaatatcg
    68761 ctaaagatcg cctgccggtt ccggctccaa tggcggttgg cgcgatccag acccgtctgg
    68821 tcgatcaaag cctgcgttgc gatgccaaca tcatcgtcga aaccgccagc gcccgcgatc
    68881 cgcaccactt cgccgtgttg ctgggtttcg gcgcgacggc tatttatccg taccttgcct
    68941 atgaaacgct gggccgcctg gtagacaccc acgcgattgc caaagattat cgtaccgtga
    69001 tgctcaacta ccgtaacggc atcaacaaag gcttgtacaa aatcatgtcc aaaatgggca
    69061 tctccaccat cgcctcttac cgctgctcga aactgtttga agcggtcggt ctgcacaatg
    69121 atgtagtggg cctgtgcttc cagggggcgg tcagccgcat tgggggggcg agctttgaag
    69181 acttccagca ggatctgctg aatctctcga aacgagcctg gctggcgcgc aagcccatca
    69241 gccagggcgg cctgctgaaa tacgtccacg gcggcgaata ccacgcctac aacccggacg
    69301 tggtgcgcac gctgcaacag gcggtacaga gcggcgagta tagcgactat caggaatacg
    69361 cgaagctggt taatgagcgt ccggcaacca cgctgcgcga tctgctggca attacgccgg
    69421 gtgaaaacgc ggtcaacatt gccgatgttg aaccggcaag cgaactgttt aaacgcttcg
    69481 ataccgcggc gatgtctatc ggcgcgttaa gcccggaagc ccacgaggcg ctggcggaag
    69541 cgatgaacag catcggcgga aactcgaact ccggtgaagg tggtgaagac ccggcgcgct
    69601 acggcaccaa caaagtgtcg cgcatcaagc aggtggcttc cggtcgcttt ggggttactc
    69661 cggcgtatct ggtcaatgcc gacgtcattc agattaaagt cgcccagggc gcgaagccgg
    69721 gcgaaggcgg tcagttgccg ggcgacaaag tcactcctta cattgccaaa ctgcgctatt
    69781 cggtgccggg cgtgacgctg atctccccgc cgccgcacca cgatatctac tctatcgaag
    69841 acttagcgca gctcattttc gacctcaagc aggttaaccc gaaagcgatg atctccgtga
    69901 agctggtttc cgaaccgggc gttggcacca tcgccaccgg tgtggcgaaa gcttatgccg
    69961 atttaatcac catcgcaggc tatgacggcg gcaccggcgc aagtccgctt tcatcggtga
    70021 aatacgcagg ctgtccgtgg gagctgggac ttgttgaaac acaacaggcg ctggttgcca
    70081 acggcttgcg tcataaaatc cgtttgcagg tcgatggcgg cctgaaaacg ggtgtcgata
    70141 tcatcaaagc ggcgattctc ggcgcagaaa gcttcggctt cggcactggc ccaatggtcg
    70201 cgctcggctg taaatatctg cgtatttgcc acctgaacaa ctgcgcaacg ggtgtggcaa
    70261 ctcaggatga caaactgcgt aagaatcact atcacggcct gccgttcaag gtgacgaatt
    70321 actttgagtt tatcgcccgt gaaacccgcg agctgatggc acagcttggc gtaacacgtc
    70381 tggtggatct gattggtcgc accgacctgc tgaaagagct ggacggtttc accgccaaac
    70441 agcagaaact ggcgctgtcg aagctgctgg agaccgccga accgcatcca ggtaaggcac
    70501 tctactgcac cgaaaccaac ccgccgtttg ataacggcct gctgaacgcg cagttgctgc
    70561 aacaggcgaa accgtttgtc gatgagcgcc agagcaaaac cttctggttc gatatccgca
    70621 acaccgaccg ctctgtcggc gcgtcgcttt caggctatat cgcccagacg cacggcgatc
    70681 aggggctggc agccgatccg attaaagcgt acttcaacgg caccgcaggc cagagcttcg
    70741 gcgtgtggaa cgcgggcggc gtggaactgt acctgaccgg tgatgccaac gactatgtcg
    70801 gtaaaggcat ggcgggcggc ttaatcgcca ttcgtcctcc ggttggttcc gccttccgca
    70861 gccatgaagc aagtattatc ggcaacacct gcctgtatgg agcgacgggt ggtcgtctgt
    70921 atgccgcagg ccgcgcgggt gaacgtttcg gcgtgcgtaa ctccggtgca atcaccgtgg
    70981 tagaaggcat tggcgacaac ggctgtgaat atatgacggg cggtatcgtc tgtattctgg
    71041 gtaaaaccgg cgttaacttc ggtgcgggta tgaccggcgg cttcgcttac gttctcgatg
    71101 aaagcggcga tttccgcaaa cgcgttaacc cggaactggt ggaagtatta agcgttgacg
    71161 atttggcgat ccatgaagag catctgcgtg gtcttatcac cgagcatgtg cagcataccg
    71221 gttcccagcg tggtgaagag attctggcga actggtcaac cttcgccact aaatttgcgc
    71281 tggttaaacc gaagtccagt gatgtcaaag cactgctggg tcatcgtagt cgtagcgcag
    71341 cagagttgcg cgtgcaggcg cagtaagggg tagcaacaat gagtcagaat gtttatcaat
    71401 ttatcgacct gcagcgcgtt gatccgccaa agaaaccgct gaagatccgc aaaattgagt
    71461 ttgttgaaat ttacgagccg ttttccgaag gccaggccaa agcgcaggct gaccgctgcc
    71521 tgtcgtgcgg caacccatac tgcgagtgga aatgcccggt acacaactac atcccgaact
    71581 ggctgaagct cgccaacgag gggcgtattt ttgaagcggc agaactgtcg caccagacca
    71641 acactctgcc ggaagtatgc ggacgtgtct gcccacaaga ccgtctgtgc gaaggttcct
    71701 gcaccctgaa cgatgagttt ggcgcggtga ccatcggcaa cattgagcgt tatatcaatg
    71761 ataaagcgtt cgaaatgggc tggcgtccgg atatgtccgg cgtgaaacag accggtaaaa
    71821 aagtggcgat tatcggcgca ggtccggcag gtctggcgtg tgcagatgtc ctgacgcgca
    71881 acggcgtaca agcggtcgtc ttcgaccgtc acccggaaat tggcggcttg ctgaccttcg
    71941 gtattccggc tttcaaactg gaaaaagagg taatgacgcg ccgccgtgaa atcttcaccg
    72001 gcatgggtat tgaattcaaa ctcaataccg aagtgggccg cgacgtacag ctggacgatc
    72061 tgctgagtga ttacgatgcc gtgttccttg gcgtcgggac ttatcagtca atgcgcggcg
    72121 ggctggaaaa cgaagacgcc gatggcgtgt acgcagcgct gccgttcctt atcgccaaca
    72181 ccaaacagtt aatgggcttt ggcgaaactc gtgaagaacc gttcgtcagc atggaaggca
    72241 aacgcgtggt agtacttggc ggtggcgata ccgcaatgga ctgcgtgcgc acgtctgtac
    72301 gccagggggc aaagcacgtt acctgtgcct atcgtcgtga tgaagagaac atgccgggtt
    72361 cccgccgcga agtgaaaaac gcgcgggaag aaggtgtaga gttcaaattc aatgtccagc
    72421 cactgggaat tgaagtgaac ggtaacggca aagtcagcgg cgtaaaaatg gtgcgtactg
    72481 aaatgggcga accggatgcc aaaggccgtc gccgcgcgga gatcgtggca ggttccgaac
    72541 atatcgttcc agcagatgcg gtgatcatgg cgtttggttt ccgtccacac agcatggaat
    72601 ggctggcaaa acacagcgtc gagctggatt cgcaaggccg catcatcgcc ccggaaggca
    72661 gcgacaacgc tttccagacc agcaacccga aaatctttgc tggcggcgat atcgtccgtg
    72721 gttccgatct ggtcgttacc gctattgccg aaggtcgtaa ggcggcagac ggcattatga
    72781 actggctgga agtttaagct ctgtcccagc gccgcctcct cagaaaagag tcggcgctga
    72841 aattctcctt tcgttctgga aagcgcctcg cataatctga agaaaaccaa tgtaatacgc
    72901 acatctggat tgttaccggc agatgcccgg cgtatagctg attccattat tcaacactgc
    72961 gccatataaa gggaaagata atggaatcgc tctcagagag aacctcaaca ggctaccagc
    73021 aaatccacga cggtattatt cacctggtcg atagcgcccg gacggaaacg gtacgtagcg
    73081 ttaacgcgct gatgaccgcg acatactggg aaattggccg acgaattgtc gaatttgaac
    73141 aaggtggcga ggccagggct gcgtatggtg cgcagctaat caagcgacta tcaaaggatt
    73201 taagtctaag gtataagcgt gggttctctg caaaaaactt acgccaaatg aggctttttt
    73261 acctcttttt tcaacatgtt gaaattcgcc agacagtgtc tggcgaatta acacctgccc
    73321 tggtccactt acgtccgttt actttccgtt aaaaacgctg acacccgcag cttttatgaa
    73381 aaagagacgc tccgctgtgg ctggtctgtt cgccagctag aacggcaaat tgcgacccaa
    73441 ttttatgagc gaacactgct gtcacatgac aaatcagcca tgctgcaaca acacgctcct
    73501 gccgagacgc atattcttcc gcaacaggcg atacgcgatc cctttgtgct cgaatttctg
    73561 gaattgaaag atgaatactc agaatccgat tttgaggagg cgctgatcaa ccacctgatg
    73621 gatttcatgc tggaacttgg ggatgatttt gcctttgttg gtcggcagcg aaggttacgc
    73681 attgatgaca actggtttcg tgtcgatctg ctgtttttcc accgccgttt acgctgcctg
    73741 ctaatcgtcg atctaaaagt gggcaaattc agctatagcg atgccggaca gatgaatatg
    73801 tatctcaact acgccaaaga gcactggacg ctaccggatg aaaatccgcc catcggtctg
    73861 gttctctgtg cagagaaagg agtcggagaa gcgcattatg cactggctgg tttgcctaac
    73921 accgttctgg cgagtgaata taagatgcaa ctacctgatg aaaaacgact cgcggatgaa
    73981 ctcgttcgaa cacaggcggt gctagaagaa ggctatagac tccgttaatt tcagcggttt
    74041 cgaattctgc agacactgtc tgcagaatct ttaaaacgaa ctaagtttta agccattaaa
    74101 tcagccttaa ccttcaccac caccttttta atctcaccag gttcgccgac aacgcatcct
    74161 ggtttatgcg gttctcccgg cataaacacg gcgaacattc ccggttttaa gttgatggtt
    74221 tgctcgtttt caatggcgct gcaaagctgg taatcatctt catggtggaa ctcttcacac
    74281 tggcgcgcag tgcctgccgt gccaaacaga atccgttcct caccttttaa taacagctgg
    74341 atatcaatgt attgctcgtg caattccgct tttttctcga cgggcgattg agtgttaaac
    74401 gtcatgacat tcataaaaat attgtcgccc tgtaattcgt aacgacccgg cgctttttct
    74461 tgcggtctgg cagctaatgc cagcgttaac gcgtcctgta acgcaggatg taacccagca
    74521 gacggtaatg actgtacttc acccatcatc ataatttttc tccctgggcc aacagcgcag
    74581 ccccaagtaa acctgcatca tggcggtaat gcgccgccag taaatcaaca tgaaatgccg
    74641 ctggctcctg cgccagatac gtttccacca gcgccagata cccttctgcc agaccaacgc
    74701 tgccaccgac caccacgcac tgacaatcag ttgtggcttt aatatcagcg atcagccttg
    74761 ccagcacatg tgcggagcgg tgaatcagct gctgcgcctg ctcgtcgccc tgcccggcgc
    74821 gcatgaaaat agttttcgca tccgcgccag ccaactcccc ctgcgctgcc gctgcaatac
    74881 cgcgaccaga ggcaatcgct tccacgcaac ctgtgcgtcc acagccgcag actgggccgt
    74941 gtggatcggc aagcgtatgc ccgatgtgcc ccgccagacc gccagggccg gtaagcagtt
    75001 tgccgccgct cactacaccg ccgccgacgc cagtggaaac ggtgataaac accatatcgg
    75061 ttatatcgcc ttccagcgcc tgatactctg cccatgctgc ggcctgggcg tcgttaatgg
    75121 caatggtcgg caaatcggta agttgcccca gcgttttgac taacggaaag tgtagcaatc
    75181 caccaagatt atgcggattc agcgccagca aactgccgtc gcgaataatt ccggttgaag
    75241 cgatggcaac ccgctgcgca tgagcttgca acggagagac taatgcggat aaggcatcac
    75301 gcaaggcttc tggtgtctgg ctggctggcg taggaagttc acgacgatcg cggatctgcc
    75361 cgtcagcgcc aatcagcgcg gcggcaagtt tagtaccgcc gatatcaatc gccagtgtgg
    75421 tcatagcacc gcctttttca tcgctgtgtt gtaccactga caaatgtgtt caagacgcgt
    75481 aatggcagaa ccgaccgtca ccgcccacgc gccgtggcgc atcgcatcag ccgcctgagc
    75541 aggcgtgttg taacgccctt cggcaatcac ccgacatccg gcgtcgctca accttttcac
    75601 cagcgtcaga tccggctctt ctggcgtttc aggcgtggta tagccagaaa gcgtagtgcc
    75661 aataatttcg gctcccagct tttggcatgc caggccgtct tccggcgttg agcagtcggt
    75721 catcgccagt aaaccgtgat gatgaatacg tgccagcagc gtttcaacag gcaccggacg
    75781 cgggcggtcg gtgccgtcaa tggcgataat gtccgcgccc gcctgcgcca gcgcatcgac
    75841 atcttcaata taggccgtga tgcgtaccgg agaatcctcc agatcgcgtt tcacaattcc
    75901 aataatcggc acgctcacca ccgcacgcgt ggcttgcaga tttgccacac cttcgatgcg
    75961 aatggcaacc gcgcccgcct gctctgccgc taatgccatg gctgcgacga tttcgggttt
    76021 atcgagcggg ctgtccggaa ccggctggca ggagacaatc aggccaccgt tagcagcgat
    76081 tttttgatcc agttgtgcaa gtaacgacat acatcttccc ttagcgaaag gcccgataca
    76141 gagaccgggc aacaggatta acttttggtt ttgactaaat cgtttttggc gctgccaaac
    76201 ggcacggcac cgctaaatgg tttgccgtcg atagcgtcat gagtacgcaa cgcttccggg
    76261 cgcagccaac gctgaacacg agaaggcata tccagaccaa tcagcaggat caccacaaat
    76321 gtcagactga acgagagcga tgccagtgca gtacccagat ccagacgttg agcgatcaac
    76381 gcgccgatga ttggggccag tgcgccaccc aatgcgccaa cgttataggt aaagcccagg
    76441 cccgctgcac gctggtcggt atcgaaataa ccgccaatca gtttcggtaa gatcccggcg
    76501 atcccttgtc caagcatttg ctggaagaac agtaacagac cgagcaccca gacgtttgca
    76561 ccgccaatag caaataccgg aataatcagc agctgcgagg ccaacagact acaaacgtac
    76621 gctttgcggg ttcccagcca gtcaccgagg aagccaccga cgcagcaccc caccgccgcg
    76681 ccaaagccac taaagaacag cacattggcg acagtatgag ggttataagc cagatcggtt
    76741 ttcagatacg ttggcagcag cgcctgaatc ggccatgagt agaggaaagc aaacaacacg
    76801 accaccatca gcattacgcc agttggccag cgtttgcccg tactctgcac cataaagctg
    76861 ataaagattg cggtgcataa cagcccaaga acagcgacga tcgcggcatt ttgcaggtta
    76921 ccggcgaagc agaaccacag cgcagtagcc gccgccagtg tcattacgat attggcaatg
    76981 cgatgttcac cacggtagag aatatccacc attgtgcgta ctggtgcttt acctgcgtgt
    77041 ttctctttcc agtcttctgc ttccgggatg tttttacgca gccagagagc aaagatgatt
    77101 ggcaaaatgc cgataaagaa cagcgcacgc cagccccaga ccggaaccac caggctgtag
    77161 acctgagcgg caacgacggc ccctacagag aagcctgaaa tcagaaagcc actggctttg
    77221 ttacgcagat gttttggcca gctttcaatg acataggtgg cgctggaacc gtattcaccc
    77281 gccatcccca tgccgatgac cagacgagcg ataaacatgg tgatgtagcc tggcgcaaag
    77341 ccgcatgcca gcgtcccggc cgagaagaga acgatgctgg tgaccattgc cagacgacgc
    77401 ccgtagcggt cacccatagc gccgagcatc aggccgccga accagcgaga gataaaggct
    77461 gcggagatca gacttgccgc ctgcaccgtc gtcagcccga attcaccttg tacttcggtg
    77521 agtaccaggg cgattaaaac gaaatcaaaa ccgtcaagca gatatcccaa ccaggcagcg
    77581 gaaaatgccc gccattgtgc acgattgaga tggcgatacc acgggatatt ctgggttgta
    77641 gtactcattg tgagtctccc gcggtgggcg atgcccacac gctttggtat gaaaattgta
    77701 gggtacagat gcgtttattt cccctcaccc ggtaggggcg agcgagggga aacaactcac
    77761 ccgcgctctt gcatcaactg ctgggccagc gccttcagtt ctggcagata tttttcatct
    77821 accggaccaa acggtttgcg gcacagcggc acagaaacga catccatata atgcagaaca
    77881 gttttcaggc cgcggaatac acccgttttg atcagtaaat caatgacttt gttgcattca
    77941 gtttgcagtt tctgcgcggt ctggatatcg ccttctttca gcgccttaac gatcccctga
    78001 tagcgccagc ccatgatgtt gtaggtacta ccgataccac catcagcgcc cgccagcagg
    78061 ccagaggcga agatttcgtc gtaaccgtta tagagcacca gatcaggatg ttcacgacgg
    78121 atctgctcca tctgatagag atcgccagag gtctgtttca gcgcacctac gccaggcaat
    78181 gtaacaagtg tgttgatctg atccagggtc agttttaccc cactcagggc tggaatgttg
    78241 tacaccacca tcggcaaacc atccgccgaa tcaataattg cccgatagtg atcgcagtgt
    78301 tcttcaaagc tgaaaggata gtagaacggc gtgacggcgg agacggcatc gaagccataa
    78361 cgtttagccg atgccgcaag ttgttggctt tcggcggtgc tgacgcaacc gacgtgggcg
    78421 atgagtttaa tcttaccttt cgcctcttcg gcgacgattt ccagtacctg ttcacgctcg
    78481 gaaaggcttt gtacaaaggc ttcgccggtc gaaccaccca cgtataaacc gtcaatgccc
    78541 tgctgaatat tgaactgaac caggcggcgc agactcgctt tatccagtgc ttgttgttga
    78601 tcaaaaggag tcaggagtgc agccattacg ccacgtaaat tcgttgccat aaatacctct
    78661 gaagtgatgc ttgtctgata aacgatatac ctttatacct gttataccag atcaattaag
    78721 caacacccca tacagaaagc ttataatgcg atctgcttca ctaaagtggc attatttctt
    78781 tttgttggtg gtctgaccga aggcgtgcca ggtagcagag acgctgttga gatgcgattg
    78841 caacgcacga tcggcttcgt caggatcgtg gcggcggatc gcgtcaacga tcgcaatatg
    78901 ctgttgatag ctaacgttgt tatgctcgtg cagagcctga tcagccacgg ttggacgtgc
    78961 ggcgatcagc cagtcgagca gggcaacgtg gatcgccatg aagattgggt taccggggat
    79021 ctccgccagc acgcggtgga aatcaacgtc tgagcgaatg aatgccgcgt tgttatccag
    79081 cgactgactg ttgatttcca gtgcttttgc cagcaaatcg atttgctcat cggtggcgtg
    79141 ttcagccgca tagcgcacca ggctggattc aaagaacaga cgtaattgtt cgaaatgggc
    79201 aatcccacca ggatgagaaa ggaaatcttt cgccatgccg gaaagctcgc cgatgatggt
    79261 gtccgcagaa ggacgtgaga cgcgggcacg ttcgccgttg tttatttgca ccagaccttt
    79321 gcgttttaac gctgccagcg cttcacgcac cgaaggacgc ccgacgttaa agaacgccat
    79381 cagttcgcgt tcagacggta attgttcacc ttcgccaaat tcacgacggc ggatcatctg
    79441 ttccagctct tcttccacca tttcggagag ttttttacgc gccagcgggc ggctacgcaa
    79501 gttgcgacca attgcaggtg aagaatcttc ggtttgcgaa tcaaatgcgt tcataaggcc
    79561 cattctgtaa ggtcagtgtg attaacatca tcagtgacat cctatcacag gattgaaagt
    79621 aggggaaaat ggcagggttt tctctttgtg cctcatcatt accataatta acggaataat
    79681 taactattgc gaaaaattaa tgtaacgcag ataaaaacat cccgtttgaa ttatttataa
//
LOCUS       DAZKIP010000011        78963 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZKIP010000011 DAZKIP010000000
VERSION     DAZKIP010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 78963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.011"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(102..1052)
                     /gene="dusC"
                     /locus_tag="ACHOUK_000867"
     CDS             complement(102..1052)
                     /gene="dusC"
                     /locus_tag="ACHOUK_000867"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFP9896372.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQECMAISGC
                     DSVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIGKLR"
     gene            complement(1212..2405)
                     /locus_tag="ACHOUK_000868"
     CDS             complement(1212..2405)
                     /locus_tag="ACHOUK_000868"
                     /EC_number="1.14.13.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybenzoate 6-monooxygenase"
                     /protein_id="HFP9896373.1"
                     /translation="MTKVTRAVIVGGGIGGAATALSLARLGIKVVLLEKAHEIGEIGA
                     GIQLGPNAFSALDSLGVGEVARQRAVFTDHITMMDAVNAEEVVCIETGQAFRDHFGGP
                     YAVIHRVDIHASVWEAVLTHPDVEYHTSTNVVDIRETEDDVTVFDDRGNSWTGDILIG
                     CDGVKSVVRQSLLGDAPRVTGHVVYRAVIDRADMPEDLRINAPVLWAGPHCHLVHYPL
                     RGGKQYNLVVTFHSRQQEEWGVKDGSKEEVLSYFEGIHPRPRQMLDKPTSWRRWSTAD
                     REPVAKWGTKRITLVGDSAHPVAQYMAQGACMALEDAVTLGKALAQCEGDAAQAFALY
                     ESVRIPRTARIVWSTREMGRVYHAAGVERQVRNLLWKGKSQAEFYRGMEWLYGWKEDN
                     CLQPR"
     gene            complement(2420..3064)
                     /gene="maiA"
                     /locus_tag="ACHOUK_000869"
     CDS             complement(2420..3064)
                     /gene="maiA"
                     /locus_tag="ACHOUK_000869"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HFP9896374.1"
                     /translation="MKLYSFFNSSASYRVRIALALKGINYQTEGVNIRIGQQNELAYR
                     RMNPVGLVPTLLTDEGESLGQSLAIIDWLERHYPQVPLVPQEEPARNKVLEIVYAIAC
                     DIHPLNNLRVLRYLTEELNVSEEEKKRWYAHWIQQGLSAVEQLLRQNQSGQFCVGETP
                     TLADCCLVPQWANALRMNCDLSGYPRCKAVYDACTQLPAFIAAAPENQQDKISA"
     gene            complement(3073..3774)
                     /locus_tag="ACHOUK_000870"
     CDS             complement(3073..3774)
                     /locus_tag="ACHOUK_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFP9896375.1"
                     /translation="MTQYVFEPQAPVTVPVVGSDEQFPVRRVYCVGRNYAAHAREMGF
                     DPDREPPFFFCKPADAVVPVAAGETLALPYPAQTDNYHYEIELVVAIGKKGSDIPLEH
                     AHEYVWGYATGLDMTRRDRQMEMRQMGRPWEIGKAFDLSAPIAPLHKARDIGGIDNAP
                     IWLQVNGEDHQRSDIRHLIWSVNETICYLSGFFELQPGDLIFTGTPEGVGPVVKGDVI
                     TGNVEGLTPIAVKIV"
     gene            complement(3789..4817)
                     /gene="gtdA"
                     /locus_tag="ACHOUK_000871"
     CDS             complement(3789..4817)
                     /gene="gtdA"
                     /locus_tag="ACHOUK_000871"
                     /EC_number="1.13.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047922 - gentisate 1,2-dioxygenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HFP9896376.1"
                     /translation="MTDNNQNSREQFYQHISGQNLTPLWESLHHLVPKTPNANCAPAY
                     WNYQEIRPLLLESGGLIGAKEAVRRVLVLENPALRGQSSITATLYAGLQLIMPGEVAP
                     SHRHNQSALRFIVEGKGAFTAVDGERTPMNEGDFILTPQWRWHDHGNPGDEPVIWLDG
                     LDLPLVNILGCGFAEDYPEEQQPVTRKEGDYLPRYAANMLPLRHQTGNSSPIFNYRYD
                     RSREVLHDLTRLGDADEWDGYKMRYVNPVTGGYPMPSMGAFLQLLPKGFASRVARTTD
                     STIYHVVEGSGQVIIGNETFSFSAKDIFVVPTWHGVSFQTTQDSVLFSFSDRPVQEAL
                     GLFREARY"
     gene            complement(4829..6187)
                     /locus_tag="ACHOUK_000872"
     CDS             complement(4829..6187)
                     /locus_tag="ACHOUK_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9896377.1"
                     /translation="MTQRRDLQALIDAAPVGKMQWRVIICCFLVVMLDGFDTAAIGFI
                     APDIRTHWQLTAGDLAPLFGAGLLGLTAGALLCGPLSDRFGRKRVIELCVFLFGALSL
                     ASAFSPDLQTLVFLRFLTGLGLGGAMPNTITMTSEYLPARRRGALVTLMFCGFTLGSA
                     FGGIVSAQLVPVIGWHGILVLGGVLPLMLFVALLVVLPESPRWQVRRQLPQAVIAKTV
                     SAITRERYVDTHFYLIESASVTKGSIRQLFMGRQLPITLMLWVVFFMSLLIIYLLSSW
                     MPTLLNHRGIDLQHASWVTAAFQIGGTLGALALGVLMDKFNPFRVLTLSYAIGAICIV
                     MIGLSQDGLWLMALAIFGTGIGISGSQVGLNALTATLYPTQSRATGVSWSNAIGRCGA
                     IVGSLSGGVMMAMNFSFDTLFFIIAVPAAISAVMLTLLITVVRQSTSVPDSLPRAGVV
                     NE"
     gene            6314..7222
                     /locus_tag="ACHOUK_000873"
     CDS             6314..7222
                     /locus_tag="ACHOUK_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9896378.1"
                     /translation="MANWAQKLKLHHLEMLVALGEQGNLTHVARMMNITQPALSKWLS
                     QLEDEIGITLFERHSKGLRPTEGGKLLLQHAQRLINDMERSQHELARFKQGGLVGSLK
                     IGCSPVATDCVSQAILHLLTKMPTLHLNIEEKVMTPLLQDLLAGSVDVVVGRIGGRAL
                     QLPLNYQVLYTEPVCFVARPEHPLAKYATLSWNDLANWRWIVWPTGTPIRISIDNALV
                     DHGVMLPENTIESASMNVSTNLLQSSDMISILSLRLAQRYASQGQLVILNLPKIEQKG
                     SVGVFWRNNETPSYALSQFLHFLQQN"
     gene            7353..7751
                     /gene="yohJ"
                     /locus_tag="ACHOUK_000874"
     CDS             7353..7751
                     /gene="yohJ"
                     /locus_tag="ACHOUK_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFP9896379.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAI
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            7748..8443
                     /gene="yohK"
                     /locus_tag="ACHOUK_000875"
     CDS             7748..8443
                     /gene="yohK"
                     /locus_tag="ACHOUK_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFP9896380.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APILFPIILAVMG"
     gene            8573..9457
                     /gene="cdd"
                     /locus_tag="ACHOUK_000876"
     CDS             8573..9457
                     /gene="cdd"
                     /locus_tag="ACHOUK_000876"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFP9896381.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LADAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHNIDRVLLA"
     gene            9607..10326
                     /gene="sanA"
                     /locus_tag="ACHOUK_000877"
     CDS             9607..10326
                     /gene="sanA"
                     /locus_tag="ACHOUK_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFP9896382.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            10329..10568
                     /gene="yeiS"
                     /locus_tag="ACHOUK_000878"
     CDS             10329..10568
                     /gene="yeiS"
                     /locus_tag="ACHOUK_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFP9896383.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            10762..12000
                     /gene="preT"
                     /locus_tag="ACHOUK_000879"
     CDS             10762..12000
                     /gene="preT"
                     /locus_tag="ACHOUK_000879"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFP9896384.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLLRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDDFAKLEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            11994..13229
                     /gene="preA"
                     /locus_tag="ACHOUK_000880"
     CDS             11994..13229
                     /gene="preA"
                     /locus_tag="ACHOUK_000880"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFP9896385.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(13472..14482)
                     /gene="mglC"
                     /locus_tag="ACHOUK_000881"
     CDS             complement(13472..14482)
                     /gene="mglC"
                     /locus_tag="ACHOUK_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFP9896386.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQAPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(14498..16018)
                     /gene="mglA"
                     /locus_tag="ACHOUK_000882"
     CDS             complement(14498..16018)
                     /gene="mglA"
                     /locus_tag="ACHOUK_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFP9896387.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYKKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(16079..17077)
                     /gene="mglB"
                     /locus_tag="ACHOUK_000883"
     CDS             complement(16079..17077)
                     /gene="mglB"
                     /locus_tag="ACHOUK_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFP9896388.1"
                     /translation="MNKKVLTLSAVMASMLFGATAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(17356..18396)
                     /gene="galS"
                     /locus_tag="ACHOUK_000884"
     CDS             complement(17356..18396)
                     /gene="galS"
                     /locus_tag="ACHOUK_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFP9896389.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYTHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            complement(18538..19695)
                     /gene="yeiB"
                     /locus_tag="ACHOUK_000885"
     CDS             complement(18538..19695)
                     /gene="yeiB"
                     /locus_tag="ACHOUK_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFP9896390.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVIINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMQFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGPAISKTSR"
     gene            complement(19712..20380)
                     /gene="folE"
                     /locus_tag="ACHOUK_000886"
     CDS             complement(19712..20380)
                     /gene="folE"
                     /locus_tag="ACHOUK_000886"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFP9896391.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            20638..21474
                     /gene="yeiG"
                     /locus_tag="ACHOUK_000887"
     CDS             20638..21474
                     /gene="yeiG"
                     /locus_tag="ACHOUK_000887"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFP9896392.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSRYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(21506..23485)
                     /gene="cirA"
                     /locus_tag="ACHOUK_000888"
     CDS             complement(21506..23485)
                     /gene="cirA"
                     /locus_tag="ACHOUK_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFP9896393.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRNGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFTA
                     NGVPVFSYYNVNKARIQGVETELKIPFNDEWKLSLNYTYNDGRDVSNGENKPLSDLPF
                     HTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVTKDVK
                     LRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(23777..25246)
                     /gene="lysP"
                     /locus_tag="ACHOUK_000889"
     CDS             complement(23777..25246)
                     /gene="lysP"
                     /locus_tag="ACHOUK_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFP9896394.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPGKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(25451..26332)
                     /gene="yieE"
                     /locus_tag="ACHOUK_000890"
     CDS             complement(25451..26332)
                     /gene="yieE"
                     /locus_tag="ACHOUK_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFP9896395.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            26431..27480
                     /gene="yeiH"
                     /locus_tag="ACHOUK_000891"
     CDS             26431..27480
                     /gene="yeiH"
                     /locus_tag="ACHOUK_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFP9896396.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            27554..28411
                     /gene="nfo"
                     /locus_tag="ACHOUK_000892"
     CDS             27554..28411
                     /gene="nfo"
                     /locus_tag="ACHOUK_000892"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFP9896397.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARTVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            28414..29502
                     /gene="yeiI"
                     /locus_tag="ACHOUK_000893"
     CDS             28414..29502
                     /gene="yeiI"
                     /locus_tag="ACHOUK_000893"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFP9896398.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIRYPQSASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDET
                     VLAINDTHLLEQLSPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLAGEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(29558..30808)
                     /gene="nupX"
                     /locus_tag="ACHOUK_000894"
     CDS             complement(29558..30808)
                     /gene="nupX"
                     /locus_tag="ACHOUK_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFP9896399.1"
                     /translation="MDVMRSVLGMVVLLTIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESIVGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(30908..31849)
                     /gene="rihB"
                     /locus_tag="ACHOUK_000895"
     CDS             complement(30908..31849)
                     /gene="rihB"
                     /locus_tag="ACHOUK_000895"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFP9896400.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGDTGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDSDWFWGLVEECVRGYIKTH"
     gene            31979..32677
                     /gene="yeiL"
                     /locus_tag="ACHOUK_000896"
     CDS             31979..32677
                     /gene="yeiL"
                     /locus_tag="ACHOUK_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFP9896401.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(32748..33998)
                     /locus_tag="ACHOUK_000897"
     CDS             complement(32748..33998)
                     /locus_tag="ACHOUK_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFP9896402.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVAAALLLQIALGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATELSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(34092..35009)
                     /gene="psuG"
                     /locus_tag="ACHOUK_000898"
     CDS             complement(34092..35009)
                     /gene="psuG"
                     /locus_tag="ACHOUK_000898"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFP9896403.1"
                     /translation="MSELKISPEVQDALKNKKPVVALESTIISHGMPFPQNAQTAIEV
                     EETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFVVAAGKNG
                     ATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTVVCAGAKS
                     ILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFDVSIRLDSASEIARAMAVKWQS
                     GLNGGLVVANPIPEQFAMPEHTINAVIDQAVAEAEAQGVIGKESTPFLLARVAELTGG
                     DSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            complement(34997..35938)
                     /gene="psuK"
                     /locus_tag="ACHOUK_000899"
     CDS             complement(34997..35938)
                     /gene="psuK"
                     /locus_tag="ACHOUK_000899"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFP9896404.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRERLSQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            complement(36362..38053)
                     /gene="fruA"
                     /locus_tag="ACHOUK_000900"
     CDS             complement(36362..38053)
                     /gene="fruA"
                     /locus_tag="ACHOUK_000900"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFP9896405.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATSE
                     GKNESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(38070..39008)
                     /gene="fruK"
                     /locus_tag="ACHOUK_000901"
     CDS             complement(38070..39008)
                     /gene="fruK"
                     /locus_tag="ACHOUK_000901"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFP9896406.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(39008..40138)
                     /gene="fruB"
                     /locus_tag="ACHOUK_000902"
     CDS             complement(39008..40138)
                     /gene="fruB"
                     /locus_tag="ACHOUK_000902"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFP9896407.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVVEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            40506..41687
                     /gene="setB"
                     /locus_tag="ACHOUK_000903"
     CDS             40506..41687
                     /gene="setB"
                     /locus_tag="ACHOUK_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFP9896408.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGVLVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(41684..41938)
                     /gene="yeiW"
                     /locus_tag="ACHOUK_000904"
     CDS             complement(41684..41938)
                     /gene="yeiW"
                     /locus_tag="ACHOUK_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFP9896409.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            42093..42665
                     /gene="yeiP"
                     /locus_tag="ACHOUK_000905"
     CDS             42093..42665
                     /gene="yeiP"
                     /locus_tag="ACHOUK_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFP9896410.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            42888..44354
                     /gene="yeiQ"
                     /locus_tag="ACHOUK_000906"
     CDS             42888..44354
                     /gene="yeiQ"
                     /locus_tag="ACHOUK_000906"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFP9896411.1"
                     /translation="MNTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQAIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLRIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRDLVAGSISEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQARQQIAQFGAHQALLNTLKI"
     gene            44472..45458
                     /gene="yeiR"
                     /locus_tag="ACHOUK_000907"
     CDS             44472..45458
                     /gene="yeiR"
                     /locus_tag="ACHOUK_000907"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFP9896412.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVAGHLLDLPRHNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGHQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            45497..46210
                     /gene="lpxT"
                     /locus_tag="ACHOUK_000908"
     CDS             45497..46210
                     /gene="lpxT"
                     /locus_tag="ACHOUK_000908"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFP9896413.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            46623..47189
                     /gene="mepS"
                     /locus_tag="ACHOUK_000909"
     CDS             46623..47189
                     /gene="mepS"
                     /locus_tag="ACHOUK_000909"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFP9896414.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            47370..48926
                     /gene="pdeN"
                     /locus_tag="ACHOUK_000910"
     CDS             47370..48926
                     /gene="pdeN"
                     /locus_tag="ACHOUK_000910"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFP9896415.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCKQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKANIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            49008..50822
                     /gene="yejA"
                     /locus_tag="ACHOUK_000911"
     CDS             49008..50822
                     /gene="yejA"
                     /locus_tag="ACHOUK_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP9896416.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYSGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDHSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            50823..51917
                     /gene="yejB"
                     /locus_tag="ACHOUK_000912"
     CDS             50823..51917
                     /gene="yejB"
                     /locus_tag="ACHOUK_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFP9896417.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            51917..52942
                     /gene="yejE"
                     /locus_tag="ACHOUK_000913"
     CDS             51917..52942
                     /gene="yejE"
                     /locus_tag="ACHOUK_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9896418.1"
                     /translation="MSRLNPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            52944..54533
                     /gene="yejF"
                     /locus_tag="ACHOUK_000914"
     CDS             52944..54533
                     /gene="yejF"
                     /locus_tag="ACHOUK_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFP9896419.1"
                     /translation="MTQTLLAIEDLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNYAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIDVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(54537..54881)
                     /gene="yejG"
                     /locus_tag="ACHOUK_000915"
     CDS             complement(54537..54881)
                     /gene="yejG"
                     /locus_tag="ACHOUK_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFP9896420.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALGDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(55214..56404)
                     /gene="bcr"
                     /locus_tag="ACHOUK_000916"
     CDS             complement(55214..56404)
                     /gene="bcr"
                     /locus_tag="ACHOUK_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFP9896421.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(56432..57127)
                     /gene="rsuA"
                     /locus_tag="ACHOUK_000917"
     CDS             complement(56432..57127)
                     /gene="rsuA"
                     /locus_tag="ACHOUK_000917"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFP9896422.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            57276..59036
                     /gene="radD"
                     /locus_tag="ACHOUK_000918"
     CDS             57276..59036
                     /gene="radD"
                     /locus_tag="ACHOUK_000918"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP9896423.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAATRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            59161..59445
                     /gene="rplY"
                     /locus_tag="ACHOUK_000919"
     CDS             59161..59445
                     /gene="rplY"
                     /locus_tag="ACHOUK_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFP9896424.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNIQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(59584..60591)
                     /gene="yejK"
                     /locus_tag="ACHOUK_000920"
     CDS             complement(59584..60591)
                     /gene="yejK"
                     /locus_tag="ACHOUK_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFP9896425.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            60773..61000
                     /gene="yejL"
                     /locus_tag="ACHOUK_000921"
     CDS             60773..61000
                     /gene="yejL"
                     /locus_tag="ACHOUK_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFP9896426.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            61020..62780
                     /gene="yejM"
                     /locus_tag="ACHOUK_000922"
     CDS             61020..62780
                     /gene="yejM"
                     /locus_tag="ACHOUK_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFP9896427.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            62855..62931
                     /locus_tag="ACHOUK_000923"
     tRNA            62855..62931
                     /locus_tag="ACHOUK_000923"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:62889..62891,aa:Pro,seq:ggg)
     gene            complement(63032..65623)
                     /gene="yejO"
                     /locus_tag="ACHOUK_000924"
     CDS             complement(63032..65623)
                     /gene="yejO"
                     /locus_tag="ACHOUK_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HFP9896428.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVVLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGISSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGAANDPTIKGGRLIVEKDGITVLAAIEKGGLLEVKEGG
                     LAIAVDQKAGGAIKASTRVMEVFGTNRLGQFEIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTRVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     LRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMDESVTRS
                     SVTASAENFTTLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTAMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNVDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTRGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            65943..66590
                     /gene="narP"
                     /locus_tag="ACHOUK_000925"
     CDS             65943..66590
                     /gene="narP"
                     /locus_tag="ACHOUK_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFP9896429.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLHEREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(66625..67677)
                     /gene="ccmH"
                     /locus_tag="ACHOUK_000926"
     CDS             complement(66625..67677)
                     /gene="ccmH"
                     /locus_tag="ACHOUK_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFP9896430.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDATLGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(67674..68231)
                     /gene="dsbE"
                     /locus_tag="ACHOUK_000927"
     CDS             complement(67674..68231)
                     /gene="dsbE"
                     /locus_tag="ACHOUK_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFP9896431.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(68228..70171)
                     /gene="ccmF"
                     /locus_tag="ACHOUK_000928"
     CDS             complement(68228..70171)
                     /gene="ccmF"
                     /locus_tag="ACHOUK_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFP9896432.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(70168..70647)
                     /gene="ccmE"
                     /locus_tag="ACHOUK_000929"
     CDS             complement(70168..70647)
                     /gene="ccmE"
                     /locus_tag="ACHOUK_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFP9896433.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(70644..70853)
                     /gene="ccmD"
                     /locus_tag="ACHOUK_000930"
     CDS             complement(70644..70853)
                     /gene="ccmD"
                     /locus_tag="ACHOUK_000930"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFP9896434.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(70850..71587)
                     /gene="ccmC"
                     /locus_tag="ACHOUK_000931"
     CDS             complement(70850..71587)
                     /gene="ccmC"
                     /locus_tag="ACHOUK_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFP9896435.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(71629..72291)
                     /gene="ccmB"
                     /locus_tag="ACHOUK_000932"
     CDS             complement(71629..72291)
                     /gene="ccmB"
                     /locus_tag="ACHOUK_000932"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFP9896436.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(72288..72911)
                     /gene="ccmA"
                     /locus_tag="ACHOUK_000933"
     CDS             complement(72288..72911)
                     /gene="ccmA"
                     /locus_tag="ACHOUK_000933"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFP9896437.1"
                     /translation="MGMLEVRELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAV"
     gene            complement(72924..73526)
                     /gene="napC"
                     /locus_tag="ACHOUK_000934"
     CDS             complement(72924..73526)
                     /gene="napC"
                     /locus_tag="ACHOUK_000934"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFP9896438.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(73536..73985)
                     /gene="napB"
                     /locus_tag="ACHOUK_000935"
     CDS             complement(73536..73985)
                     /gene="napB"
                     /locus_tag="ACHOUK_000935"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFP9896439.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(73982..74845)
                     /gene="napH"
                     /locus_tag="ACHOUK_000936"
     CDS             complement(73982..74845)
                     /gene="napH"
                     /locus_tag="ACHOUK_000936"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFP9896440.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(74832..75527)
                     /gene="napG"
                     /locus_tag="ACHOUK_000937"
     CDS             complement(74832..75527)
                     /gene="napG"
                     /locus_tag="ACHOUK_000937"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFP9896441.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLVVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(75534..78020)
                     /gene="napA"
                     /locus_tag="ACHOUK_000938"
     CDS             complement(75534..78020)
                     /gene="napA"
                     /locus_tag="ACHOUK_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFP9896442.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQDVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(78017..78280)
                     /gene="napD"
                     /locus_tag="ACHOUK_000939"
     CDS             complement(78017..78280)
                     /gene="napD"
                     /locus_tag="ACHOUK_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFP9896443.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(78270..78764)
                     /gene="napF"
                     /locus_tag="ACHOUK_000940"
     CDS             complement(78270..78764)
                     /gene="napF"
                     /locus_tag="ACHOUK_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFP9896444.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            78873..>78963
                     /gene="yojO"
                     /locus_tag="ACHOUK_000941"
     CDS             78873..>78963
                     /gene="yojO"
                     /locus_tag="ACHOUK_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFP9896445.1"
                     /translation="MHTPIEVKPVAGSKEWREAWQKRAFAHISN"
BASE COUNT        19246 a        21321 c        19514 g        18882 t
ORIGIN      
        1 tgcgccagtg gcaatatgtg gcttagctca tgaaaggagc gcaacaagat gatgaagctg
       61 aataagcgat tcattgtttc tgataaactg cgattccact attatcgcag cttaccgata
      121 tcaattgcct gaatagccct tgcaatatca ggggaattat tcaacacacg aacatgctga
      181 aataattctg ttgcttcatc gtattcttta cgcaaataac tcaaccactg tttaatccgc
      241 gctacgtgat ataacccggt atcgccctgc ttttccagac gggtatattt ttgcagcaaa
      301 gcaaccacct ccggccacgg cattcgcggt tcgttatatt ttactacccg gctcaggttg
      361 ggaatattga gcgccccgcg accaatcatc actgagtcgc agccgctgat cgccatgcat
      421 tcttgcgcgc tctgccagtc ccagatttcg ccgttggcaa tcaccggaat attcagccgc
      481 tggcgaatct cgccaatcgc ctgccagtca atatgctccg cgcggtaacc ctgctctttt
      541 gtccgtccat gcaccaccag ctccgtagca ccagcctgtt gaaccgcatc ggcgatttca
      601 aatttcttct caccgctgtc ccagcccaga cgcactttca cgctgacggg caaatgcgcc
      661 ggtacagctt cacgcatcgc ttttgcaccc tggtagatga gttcaggatc tttgagtaac
      721 gtcgccccac caccgctacc gttaaccgtt ttcgacgggc agccgcaatt gagatccacg
      781 ccccaggaac ctaattccac cgcacgggcg gcgttctctg ccagccattg tgggaactga
      841 cccaacaact gcacgcgcac cagcgtaccg gatggtgtcc ggctggcgtt ttgtagctca
      901 gggcaaatgc gatgaaagac tttcaccggc agcagttgat ccaccacgcg gacgaactcg
      961 gtgatgcaca gatcgtagtc gttaacttcg gtcagcaatt cacgcaccag agagtcaagc
     1021 actccctcca tcggagccag taacacacgc atatcatcac ccgcaaaaaa atgaggcgct
     1081 atgttagcgc ctcctgtgtt tgcagacaaa ctgacttact gattaattcg ggcaagattt
     1141 ttaccctcac cctaaccctc tcccgaaggg gcgaggggac cgcccgtgcg cctgattggg
     1201 tgaagaagtg cttagcgtgg ttgcaaacag ttatcttctt tccagccgta caaccactcc
     1261 atgccgcgat aaaattctgc ctgcgatttc cctttccaca gcagattacg tacctggcgt
     1321 tctacccctg cggcgtggta aacccgcccc atttcacggg tcgaccagac gatgcgcgcg
     1381 gtgcgcggaa ttcgtaccga ctcatacagc gcaaaggctt gcgccgcatc accctcacac
     1441 tgcgccagcg ccttacccag cgttactgca tcttccagcg ccatacaggc tccctgcgcc
     1501 atatattgcg ccaccggatg ggccgaatcg cccacaagcg ttatgcgctt cgttccccac
     1561 ttcgccaccg gttcacggtc agcggttgac cagcgtcgcc atgaagtcgg tttatccaac
     1621 atctggcgcg gacgggggtg aatcccttca aagtaagaga gcacttcctc tttactacca
     1681 tccttcacgc cccactcttc ctgctggcgg ctgtggaatg ttaccaccag gttatactgc
     1741 ttgccgccgc gcaaagggta atgcaccaag tgacaatgcg gccccgccca cagtaccggt
     1801 gcgttaatgc gcaaatcttc cggcatatcg gcacgatcga taaccgcacg gtagacaaca
     1861 tgtccggtga cacgcggcgc atcgcccaat aagctctggc gcaccactga cttcacgccg
     1921 tcacagccaa tcagaatatc gccggtccag ctattgccgc gatcgtcaaa cacggtgaca
     1981 tcgtcttccg tctcgcgaat atccacgacg ttggtggagg tgtgatattc cacgtccgga
     2041 tgcgtcagta ccgcttccca caccgaggcg tgaatatcaa cccggtggat caccgcgtaa
     2101 gggccgccga aatggtcacg aaaggcctgc ccggtttcaa tgcacaccac ttcttcggcg
     2161 ttcaccgcat ccatcatggt aatgtgatcg gtaaacaccg cgcgctggcg ggcaacttcg
     2221 ccgacgccga ggctgtccag cgccgaaaac gcatttggcc ccaactggat gcccgcgcca
     2281 atctcaccga tctcatgcgc cttttccagc agcaccactt tgatccccag gcgggccagt
     2341 gacagcgcag ttgccgcacc gccgatcccg cctcccacaa ttactgcgcg tgtcactttc
     2401 gtcattatag ttctcctggt caggctgaaa ttttatcttg ttgattttct ggcgctgcgg
     2461 caatgaacgc tggcagctgt gtgcaggcgt cgtacaccgc tttgcagcgt ggatacccgc
     2521 tcaaatcgca gttcatgcgt agcgcgttcg cccactgcgg gacaaggcag caatctgcca
     2581 gcgtcggcgt ctcgcccaca caaaattgcc ccgactggtt ctggcgcaac agttgttcca
     2641 ccgcgcttaa cccttgctgg atccagtgcg cataccaacg ttttttttct tcttcactga
     2701 cgttcagttc ttcggtcagg tagcgcagca cgcgcaagtt attcaatgga tgaatatcac
     2761 aagcgatggc atagacaatt tccagcacct tgttgcgggc cggctcttcc tgcggcacca
     2821 gcggaacttg cgggtagtgc ctctccagcc agtcaataat cgccagcgat tgtccgagtg
     2881 attcgccctc gtcagtcagc agcgtcggca ccagcccgac cggattcatc cgccgatagg
     2941 ccagttcatt ttgctgacca atacggatat tcaccccttc ggtctggtaa ttaatgcctt
     3001 tcagcgccag cgcaatgcgt acccggtacg acgctgaact attaaaaaaa ctgtacagct
     3061 tcatcatgca cctcagacaa tcttcaccgc gataggcgtt aagccctcga cgttgcctgt
     3121 gatcacatca cctttcacta ccgggccaac cccttccggc gtaccggtga agatcagatc
     3181 gcccggttgc agctcaaaga agcctgacag atagcagatg gtttcattca ccgaccagat
     3241 gagatggcga atatcgctgc gctgatgatc ttcgccgtta acctgtaacc agattggcgc
     3301 attatcaata ccgccgatat cgcgggcttt gtgcagcggc gcgatgggcg ctgagagatc
     3361 aaacgcttta ccgatttccc acgggcggcc catctggcgc atttccattt gacggtcacg
     3421 ccgagtcata tcaagacccg tggcataacc ccagacatat tcatgggcgt gttccagcgg
     3481 gatatcgctg ccttttttac caatcgccac caccagttca atttcgtaat ggtagttatc
     3541 ggtctgtgcc ggatacggca gcgccagcgt ttcaccagcg gcaaccggaa caacggcatc
     3601 cgccggtttg cagaagaaga atggcggttc acgatccgga tcaaagccca tttcgcgggc
     3661 gtgagcggca tagttacgtc cgacgcagta aacgcggcgc acggggaatt gttcatcact
     3721 gccaacaacc ggtacggtaa cgggcgcttg cggttcgaat acgtattgag tcatttttct
     3781 ctcctaaatt aataacgcgc ttcgcggaac agccccaggg cttcttgtac cggtctgtcc
     3841 gaaaaactaa ataaaacgga atcttgcgtg gtctggaacg acacgccatg ccaggtcggc
     3901 accacgaaga tatcttttgc actaaagctg aaggtttcat tgccgatgat gacctgcccg
     3961 ctaccttcca ccacatggta gatggtgctg tcagtggtac gcgcaacgcg tgaggcaaac
     4021 cctttcggca gcagttgcag gaaagcgccc atcgacggca tcggatagcc gccggtgacc
     4081 gggttgacgt agcgcatttt gtagccatcc cactcgtcgg catcgcccag tcgggttaaa
     4141 tcgtgcagca cttcgcggct gcggtcataa cgatagttaa agatcggcga ggagttcccc
     4201 gtctgatgcc gcagcggcaa catattggca gcgtaacgcg gcagataatc tccctcttta
     4261 cgcgttaccg gttgttgctc ttccggataa tcttcggcaa aaccgcagcc cagaatattc
     4321 actaatggca gatccagccc atcgagccag ataaccggtt cgtcgccagg gttaccgtga
     4381 tcgtgccagc gccactgcgg ggtcaggata aaatcgcctt cattcattgg cgtgcgttcg
     4441 ccatcaacgg cggtaaatgc ccctttgcct tcaacaataa aacgcagcgc cgactgatta
     4501 tgacgatgac tcggtgccac ttcgcccggc atgatcagtt gcaaaccggc atataaagtc
     4561 gcggtaatgg aagactgccc gcgcagcgcc gggttttcca gcaccagcac gcggcgcacg
     4621 gcctctttcg caccaatcag gccgccgctc tccagtagca gcgggcggat ctcctgataa
     4681 ttccagtacg ctggcgcaca gttagcgttg ggcgttttcg gcaccagatg gtgcagtgac
     4741 tcccacagcg gggtcaggtt ttgcccggaa atatgctgat aaaattgctc gcggctattt
     4801 tgattgttat cagtcattga gatgctcctt attcgttcac aacgcctgcg cgaggcagtg
     4861 agtcagggac agaggtcgac tggcgaacca cggtaatcag cagggttaac atcaccgcac
     4921 tgatcgccgc aggcacagcg ataatgaaaa acagggtgtc gaaagagaaa ttcatcgcca
     4981 tcatcacgcc gccagacagc gaaccaacaa tcgcgccgca gcggccgata gcatttgacc
     5041 agctcacgcc cgtggctcga ctttgtgttg gataaagtgt tgcggtcagc gcattcagcc
     5101 ccacctgcga tccgctaatg ccaatgccag tgccgaaaat cgccagcgcc atcaaccata
     5161 atccatcctg gcttaagcca atcatcacga tgcatatagc gcctatggca tagctcagcg
     5221 tcagcacgcg gaaaggattg aacttatcca tcagcacacc cagcgccagc gcgcctaacg
     5281 taccaccaat ctggaacgcg gcggtgaccc aggacgcgtg ttgcaggtcg atgccgcgat
     5341 ggttcagtag cgtcggcatc cagcttgaga gcaggtaaat aatcagcagg ctcataaaga
     5401 acactaccca cagcatcaac gtgatgggta actgacgtcc cataaacagt tgacgaatgc
     5461 tgccttttgt aacggaggct gattcaatga gataaaagtg ggtatcgaca taacgctcgc
     5521 gggtgatggc gctgacggtt ttcgcaatca ccgcctgcgg tagctggcgg cgcacctgcc
     5581 agcgtggcga ttccggcaga actaccagca gtgcgacaaa cagcatcagc ggtaacacgc
     5641 cccccaacac cagaatgccg tgccagccaa tcaccggcac cagctgcgca ctgacaatcc
     5701 cgccaaacgc cgaacccagc gtaaagccgc aaaacatcag cgtcaccagc gcgccgcggc
     5761 ggcgggcagg cagatactcc gaggtcatgg tgatggtgtt cggcatcgcg ccgcccagac
     5821 ccaggccggt gagaaagcgc aggaagacca gcgtttgcag atccggtgag aaagccgagg
     5881 cgaggcttaa cgcgccaaac aaaaagacgc acagctcaat cacccgcttg cgaccaaagc
     5941 gatcggacaa cgggccacaa agcagtgcgc ctgcggttaa tcctagcaga cccgcgccaa
     6001 aaagcggggc gagatcgcct gcggtgagtt gccagtgagt gcgaatatca ggggcgataa
     6061 atccgatagc cgcggtgtca aaaccatcga gcattaccac cagaaaacag cagatgataa
     6121 cgcgccactg catcttgccg accggagcag catcgataag tgcttgtaaa tcacgtcgtt
     6181 gagtcatagt aaatgcctca gtgcaggtaa ggtagatgtt tcttttgttt tgtatatgtt
     6241 gcgatattgt gattaaagag atgagatgta caatggcatt tcgggcagag agataacctt
     6301 gaggttatgg caaatggcga actgggcgca aaaattaaaa ctacatcacc ttgaaatgct
     6361 ggtggcgctg ggtgaacagg gcaatctaac ccatgtcgca cgaatgatga atatcaccca
     6421 acctgcgctt tcgaaatggt tgtcacaact cgaagatgag attggaatca cactttttga
     6481 gcggcacagc aaaggattac gtccgactga aggcggcaaa ttgttattgc aacatgccca
     6541 acggttaatc aatgacatgg agcgatcgca acatgaactg gcccgtttta aacaaggtgg
     6601 actggtcggc agtctgaaaa ttggctgttc tcccgtcgcc actgactgcg tatcccaggc
     6661 gattttgcat ttgcttacga agatgccaac tctacacctg aatatcgaag agaaggtgat
     6721 gaccccattg ctgcaagatc tattggccgg ttctgtcgat gtggtcgttg ggcgaattgg
     6781 cggtcgcgca ttgcagctgc cactgaatta tcaggtgctg tacaccgagc cagtatgctt
     6841 tgtcgcccgc cctgagcacc cactggcgaa atacgccacc ctcagctgga atgatcttgc
     6901 caactggcgc tggatcgtct ggccgacggg tacccccatt cgcattagta ttgataatgc
     6961 gctggtagac cacggcgtaa tgctgccaga aaacacgatt gagtcggcgt cgatgaacgt
     7021 cagtaccaac ctgttacaaa gcagcgatat gatctctatt ttgtcattac gcctggcgca
     7081 acgttatgcc agccagggac aactggtgat actcaacctg ccaaagatag aacagaaagg
     7141 tagcgtcggt gtgttctggc gcaataacga aacgccttct tatgctttaa gtcagtttct
     7201 gcacttttta cagcagaatt agtgtgctga acggggcgct cgcccgaggt tacctcccta
     7261 aaattcaact atgctgagaa ttcttaatgt gatcggtagc acgttttacg aattaattgt
     7321 atgatgaatc catctcatct ggggtgttga ttatgagtaa gacactgaac attatctggc
     7381 aatatttacg cgctttcgtc ctgatttatg cctgcctgta tgcaggcatt ttcattgctt
     7441 ccctgctacc ggtaaccatt ccgggcagca tcatcgggat gctgatcctg tttgtcctgc
     7501 tggccttgca aattcttccg gcgaaatggg tcaatccggg gtgctacgta ctgattcgct
     7561 atatggcgct attgtttgtg ccgattggcg taggcgtcat gcaatatttt gatttgctcc
     7621 gcgcacagtt tggcccggta gtggtttcct gtgcaatcag tacgctggtg gtttttctgg
     7681 tggtgagctg gagttcgcaa ctggtacacg gtgaacgtaa agtcgtaggt cagaaaggat
     7741 cagaagaatg atggcgaata tctggtggtc attaccgctg acgttgattg tcttttttgc
     7801 cgcccgcaaa ctggcggcac ggtataagtt tcccctgctt aacccgttgc tggtagcaat
     7861 ggtggtgatc attccttttt taatgctgac cggcatctct tacgacagtt actttaaagg
     7921 tagcgaagtg ctaaacgatc tgctgcaacc ggcggtcgtc gcgctggcct atcctttata
     7981 tgagcagcta caccagatcc gcgcgcgctg gaaatcgatc atcaccatct gttttatcgg
     8041 cagcgtggtt gcgatggtga cggggacttc cgtggcattg ttgatgggcg cttcaccgga
     8101 aatcgccgcg tcaatcctgc cgaaatcagt caccacgcct attgcaatgg cggttggcgg
     8161 cagtattggc ggtattccgg caatcagcgc agtttgcgtg attttcgtcg gcatcctcgg
     8221 cgcggtattt ggccatacat tgcttaatgc gatgcgtatt cgtaccaaag ctgcgcgcgg
     8281 tctggcaatg gggactgcct cgcacgccct cggtacggcg cgctgcgccg agctggatta
     8341 tcaggaaggt gcatttagtt cgctagcgct ggtgttatgc gggataatta cttcgctgat
     8401 cgcaccgatc cttttcccga ttattctggc agtaatgggc taaaatttgc gatgcgtcgc
     8461 gcatttttga tgtaagtttc acgcgttgca taattaatga gattcagatc acatataaag
     8521 ccataacggg ttcgtaaact gttatcccat tacatgatta tgaggcaacg ccatgcatcc
     8581 acgttttcaa accgcttttg cccaacttgc ggataacttg caatctgcac tggaacctat
     8641 tctggcagac aagtacttcc ccgctttgtt gaccggggag caagtctcat cgctgaagag
     8701 cgcaacgggg ctggacgaag acgcgctggc attcgcacta cttccgctgg cggcggcctg
     8761 tgcgcgtacg ccattgtcga attttaatgt tggcgcaatt gcgcgcggtg tgagcggaac
     8821 ctggtatttt ggtgccaata tggaatttat tggtgcgaca atgcagcaaa ccgttcatgc
     8881 cgaacaaagc gcgatcagcc acgcctggtt gagtggtgaa aaagcgcttg cagccatcac
     8941 cgttaactac acgccttgtg gtcactgccg tcagtttatg aatgaactga acagcggtct
     9001 ggatctgcgt attcatctgc cgggccgcga ggcacacgcg ctgcgtgact atctggcgga
     9061 tgcctttggg ccgaaagatc tggagattaa aacgctgctg atggacgagc aggatcacgg
     9121 ctatgcgctg actggtgatg cgctttctca ggcggcgatt gcggcggcaa accgttcgca
     9181 catgccttac agtaagtcgc caagcggtgt cgcgctggaa tgtaaagacg gtcgtatttt
     9241 cagcggcagc tacgctgaaa acgccgcatt caacccgact ctgccaccgt tgcagggagc
     9301 gttaattcta ttgaatctca agggttatga ttacccggat atccagcgcg cagttctggc
     9361 agagaaagcc gatgcgccgt tgattcagtg ggatgccacc tccgcaacac tgaaagctct
     9421 cggctgtcac aatatcgacc gcgtgcttct cgcttaagcc tggtgccgga tgcggcgtga
     9481 acgccttgtc cggcttgcca gccctctcct ggtgtcgaaa atcccggcaa acagtttgtc
     9541 gtttcttgcg caaaaccagc gggtaaagta gcctgatgga aattttcctt agatcgagtc
     9601 tcctgcatgt taaagcgcgt gttcctcagc ctgttagtcc tgatcggctt gctgctgttg
     9661 actgtgctcg gcctcgatcg ctggatgagc tggaaaaccg cgccttatat ctacgacgaa
     9721 ttgcaggatc tcccctaccg ccaggtcggt gtggtgctcg gaacagcaaa atattatcgt
     9781 actggcgtaa ttaatcagta ttatcgctac cgcattcaag gagcgattaa tgcctataac
     9841 agcggtaagg taaattatct attactgagc ggcgataacg cattgcaaag ttataatgag
     9901 ccgatgacca tgcgcaaaga tttaatcgct gctggtgtcg acccatcaga tattgttctc
     9961 gattacgcag gctttcgtac gctggattcc atcgtgcgta cacgcaaagt tttcgatact
    10021 aatgatttca ttattatcac ccaacgtttc cactgtgagc gggcattatt tattgcgctg
    10081 catatgggga ttcaggctca gtgttatgcc gtaccgtcac cgaaagatat gctgtcagta
    10141 cgtattcgtg aatttgccgc ccgtttcggt gcgctggctg acctttatat ttttaaacgt
    10201 gaaccgcgtt ttttagggcc gctggtccct attccggcta tgcaccaggt gccggaagat
    10261 gcgcaggggt atcccgccgt cacacccgaa cagttacttg aattacaaaa gaaacaagga
    10321 aagtagttat ggatgtacag cagttttttg tcgttgccgt ttttttcctt atcccgatat
    10381 tttgtttccg cgaagcatgg aaaggctggc gcgcaggcgc gattgataaa cgggttaaaa
    10441 atgcaccgga accggtgtat gtctggcgag caaaaaatcc cggactcttt ttcgcttata
    10501 tggtggcata tatcggcttc ggaattttat ctatcggcat gattgtttat cttattttct
    10561 atcgttaata ttttctcttt tctgttaatt attgagaata attattactt cactcaataa
    10621 gctgcggata taacattctg aaccgcagct atttgtgaat cctttcacag tttaaatttc
    10681 cccacgcgca tagccttaat atcagcacgc aatatttatt tagcgttcgc cttaattact
    10741 catagcatta aggaagatca catgccgcaa caaaattatc tggatgaact cactccggct
    10801 tttacgccgt tactggcgat taaagaagcc tctcgctgtt tattatgtca cgacgctccc
    10861 tgtagtcagg cttgcccggc acagaccgat ccggggaaat ttattcgctc aatctacttt
    10921 cgtaatttta aaggcgctgc cgagacaatt cgcgaaaata atgccctcgg tgccgtttgt
    10981 gccagagtgt gcccgacgga gaaattatgc caaagcggtt gtacccgtgc cggtgttgat
    11041 gctcccattg atatcggccg cttacagcgt tttgttactg attttgaaca acaaaccggg
    11101 atggaaattt atcagcccgg tactaaaacg ctcggcaaag tcgcgattat tggcgcaggt
    11161 cctgccggat tgcaggccag tgtgacgcta acaaaccagg gttatgacgt cacgatttat
    11221 gagaaagaag cgcaccccgg tggctggttg cgtaacggta ttccgcaatt tcggttaccg
    11281 caatcagtgc tggatgcaga aatcgcccgt attgaaaaaa tgggcgtgac cattaagtgc
    11341 aataacgaaa tcggtaaaac actcaccctg gagcaactga aagcggaaaa ccgcgcggta
    11401 ctggtcaccg tggggttatc aagcggttcc gggctaccgc tgtttgagca tagcgacgtt
    11461 gagattgccg tcgacttcct gctacgtgca cgacaggcgc aaggcgatat cagcattcca
    11521 caaagcgcat taattatcgg cggcggtgat gtcgcgatgg acgtagccag cacgctgaaa
    11581 gttctcggct gtcaggcggt aacttgcgta gcgcgtgaag agttagatga gtttccggca
    11641 agcgaaaaag agtttgccag cgcccgggag ctgggcgttt cgatcatcga tggattcacg
    11701 ccagtagccg tggaaggcaa taaagtcacg tttaagcatg tacggttatc gggcgaactg
    11761 acgatggcgg cagataaaat tattctcgcc gtcggtcagc atgccagact ggatgacttt
    11821 gcgaagttag agccgcagcg taacaccatc aaaacacaaa attatcagac ccgcgatccg
    11881 caagtctttg ctgctggcga tattgttgag ggtgacaaaa ccgtggtcta tgccgtgaaa
    11941 accgggaaag aagccgccga ggcgattcat cactatttag agggagcttg ctcatgttaa
    12001 cgaaagatct ttcgattact ttttgcggcg tgaagtttcc caacccgttc tgcctctctt
    12061 cttcgccggt aggcaactgc tatgagatgt gtgccaaagc ctacgacaca ggttggggcg
    12121 gcgtggtgtt taaaacgatc ggctttttta tcgccaacga agtctcgccg cgttttgatc
    12181 atctggtgaa agaagatacc ggttttatcg gcttcaaaaa tatggaacag attgctgaac
    12241 atccgttgga agagaatctg gccgccctgc gtcggctgaa ggaagattac ccggacaaag
    12301 tattgatcgc ttcgatcatg ggggaaaatg agcagcaatg ggaggagctg gcgcgcctgg
    12361 tgcaagaagc tggcgcggat atgatcgagt gtaacttctc ctgtccgcaa atgacttctc
    12421 atgcgatggg tagcgatgtc gggcaaagcc cggagctggt agaaaaatat tgtcgggcag
    12481 tgaaacgggg ttccacgctg ccaatgctgg cgaagatgac gccgaatatc ggtgatatgt
    12541 gcgaagtggc gctggcggcg aagcgcggcg gcgcagatgg cattgcggcg attaacaccg
    12601 ttaaatccat caccaatatc gatcttaatc agaaaatcgg tatgccgatc gttaacggaa
    12661 aatcgagtat ttccggatat tccggtaaag cggtaaaacc gatcgccctg cgcttcattc
    12721 agcaaatgcg cacccatcca gaactgcgcg atttcccaat cagcggtatc ggcggcattg
    12781 aaacctggga ggatgcggcg gagtttttat tgctcggcgc agcaacgtta caggtcacca
    12841 ccggcattat gcagtacggc tatcgcatcg tagaagatat ggcgagcggg ttatcgcatt
    12901 atctcgccga tcagggattt gattcgttgc aggagatggt aggtctggcg aataacaata
    12961 ttgtcccggc ggaagattta gaccgcagtt atattgtcta tccccgtatc aatcttgata
    13021 aatgtgttgg ctgtggacgc tgttatattt cctgttacga cggcggtcac caggcgatgg
    13081 aatggagcga gaaaacccgc acaccgcatt gtaataccga gaaatgtgtg ggttgtctgc
    13141 tttgtggtca cgtctgcccg gtgggttgta ttgagctcgg ggaagtgaag tttaagaaag
    13201 gcgagaaaga acacccggta acgttgtaaa accagagacg catccggcat ttggttcctg
    13261 tcgccggatg cggcttgaac gccttatccg gcctacaaaa ccatacaaat cagtatgttg
    13321 agctcattgc aggccggata aaacgcgcca gcgttgcatc cggcatttgg ctcctgtcgc
    13381 cggatgcggc gtgaacgcct tatccggcct acaaaaccac acagatcatg atgttatgtg
    13441 cgtttatgac cgaatgcgga ccacaatcat ttcacttctt acgcgcgtac ttcagtgaat
    13501 ccagcgctac ggcgaagata ataatcgccc ctttgatgat gtactgccag tatgggttta
    13561 cgccgatgta cgtcaggcca tagttgatga cggtaaaaat aattaccccg gtcaccacgc
    13621 caatcaccgt ccccacaccg ccgctgaacg atacaccgcc taccacgcac gccgcgatag
    13681 catccagttc atacataaag ccgaggttgt tggtggcaga gccgatacgt ccggcttcta
    13741 acatcccgcc aaaggcatag aacacgccag acaacgcgta gatcatcagc aggttcaggc
    13801 cgacgttgac accagatact tttgccgctt ccgggttacc gccaatggca aaaatgttct
    13861 taccgaagcg ggttttgttc cacaacaccc agacgaacgc caccgcaatc aacgcgtaga
    13921 aggtgatgta agagagacgg aaactcccca gcgcgacaaa gccctgagca aaggtagaga
    13981 agccactgtc aaaaccagaa attggcgacg ccccgacaaa gtcgtaataa agcgagttga
    14041 tgccatagac gatgatcatc gtgccgagcg tggtaatgaa cggcgtcacg ttgagataag
    14101 cgataatcag accgttgatc aaaccgatca ccgcaccaat ggcacagaca atcagaataa
    14161 ccagcgcaat cggcatcgtc gccatttccg ggaacacttt gttggcgtta tccatggact
    14221 gcaataatgt cgctgccacc actgccgcca gccctacttg acgccccgca gaaaggtcgg
    14281 tcccctgggt gacaattaac cctgccacac cgagcgcgat aataatacgc accgatgact
    14341 gggtgagaat attacttaag ttcaacagac ttaaaaatgt tggggcctgg aaaataataa
    14401 tcgcaagcaa aactaaaaga acgacgtaaa taccgccctc tttcaggtaa gtaagaaaac
    14461 ttttcttatt taacgcactc atgggaagcc cctaatctta aaggtgcaaa gacgcaagac
    14521 gtagaatttc gttttgcgtt gttgtttttg tatcgacaat tccggaaacg aggccattgc
    14581 tcataaccag aatacggtct gttatcccta acaactctgg catttcagag gagataataa
    14641 taatcccctt gcctttcttc gccagttcgg caattaactg ataaatctca aacttcgccc
    14701 caacatcaat accgcgcgtc ggttcatcga gcattaatat ttctggttgc gttaacagcc
    14761 agcgcccaat aatcaccttt tgctgattac cacctgagag cgaaccaatt tgcgtccgat
    14821 gccccggtgt ttttacccgc atcgaatcaa tcacccactg ggtatcgctt ttcatccgcg
    14881 agttatccag taaaccaact ttatttttgt agttgcgaat attggaaatt aacgagttaa
    14941 aaccaatatc cagataggca taaattcccg ttgagcggcg ctcctcagtt accagtgcaa
    15001 atccgtggtt tatggcttcg ttggcattat ggttattgat ctttttgccg tgcaacgtaa
    15061 tggtgccagc cgatttctcg cgaataccaa ataacgtctc aacaatatcg gtacgtttcg
    15121 cccccaccag accggcaata ccgaggatct cccctttatg cagatcaaac gagacatcgc
    15181 gaatcgacgg ctggcgcagt gacgtcaggt tacgtacctc gaggatgact tcgcccggct
    15241 tgttttcttt gtcaggaaaa cgctggttaa gagaacgccc aaccatcatg gcgataatct
    15301 tgtccatcgt cagccctgcc agcggttcgg tggcgatcca ctgaccgtcg cgcaatacgg
    15361 taacttcatc acataactgg aagatttcct ccattttatg cgagatataa acaataccgc
    15421 agccgcgctc ttttaattta cgaataatag tgaacagatg attgacctct ttttcggtta
    15481 acgaagaagt cggttcatcc ataatcacaa ttttcgcgtt ataggaaaac gctttggcga
    15541 tttcgatcat ctgcatttgc gaaacagata atgtgccgac gcgcgcacgc ggatcgatat
    15601 caatatccag ttcatcaaaa atcgctttgg tttcgcggta cattttgtcc tgatcgacaa
    15661 acatgccttt ggtggggtat cgcccaagcc acatgttgtc catcaccgaa cgttgtaata
    15721 ccaggtttaa ctcctggtgt accatcgaaa taccattttc cagtgcttct ttggccgaat
    15781 ggaaatcgat ctctttaccc tggaataaaa tggtgccgga gtctttttta taaataccaa
    15841 acaggcattt taataatgtt gattttcctg cgccgttttc ccccattaat gcatggatag
    15901 aatgtggccg gacttttaaa ttaacgttat caagtgcctt aacaccagga aaagacttgt
    15961 tgataccgct catttccaac aagtattccc cggaagacgg agtcgttgag ctgaccatat
    16021 aattttacct tgttggccat acaataaggg cgcagtaata gactgcgccc aatcagtctt
    16081 atttcttgct gaattcagcc aggttgtctt tatctacgcc aacataaggt acgcggacca
    16141 ctttgttgtc gattttccag ttggtgccat cagccgcacc tttaccatcg gccaggtttt
    16201 tcgccagatc aaaggtcgct ttcgcctggt tgttagcatc gttcagtacg gtgcccgcca
    16261 gtgcaccgga tttcaccagc gccagcgctt ctggcagagc atcgacgcca aacaccggaa
    16321 tgctggactt gttgtgtgct ttcagtgctt ctaccgcgcc cattgccatc gcatcgttgt
    16381 tggcgataac cacttcgatt ttgttggcgt tcgggccaga cagccaggcg tccatcttat
    16441 ctttcgcctg agcggtatcc cacatagcgg tatctaactg taactgttca gttttaatgc
    16501 ctttgtcgtt cagctcttta atcacgtaag tggtacgtgc ttctgcatcc ggatggcccg
    16561 gttcaccttt cagcagtacg aattgaatct gaccgtcttt attcagatcc caaccctgat
    16621 tcgccgccca gtgtttagca atcaaatcgc cttgaataat gccggactct ttggagtcag
    16681 tgccaacgta gtaggctttg tcgtagctat ccagcgcctt acgagacggt tctttgttaa
    16741 agaataccac cggcacattt tgcccacgcg ctttctcaat caccgtaccc gcagctgccg
    16801 ggtcaaccag gttgattgcc agtgccttca cccctttcgc cagcaatacg tcgatctgat
    16861 cgttctgctt ggactggtca ttctgtgaat cattcatcag cagctgaaca tctggcgcgg
    16921 ctttcgcatc ttgctcaata gccttgcgca ctacagacat aaagttatcg tcgtacttat
    16981 agattgttac accaatgcga gtatcagcag cgtgtgcagt ggcaccgaat aacatgctgg
    17041 ccatcacagc agacagggtt aacaccttct tattcatggt atctccggtt tttcttatgc
    17101 agggtagtgc ttgagataaa tgctcggcgg ggcagtagag ttaatgaagt gttactgaac
    17161 gccgaagctc actttttaaa attcgttctt ccatgcccgg taacgctcca gaaaacggct
    17221 ttaattgttg ttatgacgca ttacctcggt gaaagtgatt attcactgtt acatacgggt
    17281 tacaacgtta aaacggtgca atcatagcta tcacattgtt aagatactgt gaaatcactc
    17341 acagattgaa agcggttaca tcgcctgatt tgttgagtta gtgatcgccg ccgcattctg
    17401 gcgtgttgcg acagaatggc gacgcactaa cgtcggcata aaacagtggc tggcacgagg
    17461 atcaatattg cctgctgccc cctgcaaggc cagttcggtg gctaatttcg ccattgaagc
    17521 aatgggataa cgcacggtcg ttaattgcgg gtcggtgtaa cgggcgatgg gaatatcatc
    17581 gaaaccgatg attgagagat gtaacggaat cgcaatgcca ttatctttta atgctgtcag
    17641 tgcgccagca gccatattgt cgttataagc aaatacagcg gtaagttgta gattgcgccc
    17701 cagcagttca accatcgccg cctcaccgcc cggcatgtcc ggcgtaccag tgccaatcca
    17761 gctttccggc ggaataatat cctgctcttt caacgcactc atccagcctg ctttacgcat
    17821 ggcgtcatct tcaatgccgt ggctggaaga aagataacca atacgttgat gaccgttatt
    17881 cagcaacatg cgcgtcgcca ttcgggcacc gctgagatta tccaggcaaa cgcaacgatg
    17941 ggtgtacccc ggcacaacgc ggttgattaa caccataccg ggaatgttat ccataaattg
    18001 cgccagttca tcgtcactca atgcttttga gtgaacaatc aacgcattac aacgctggcg
    18061 aattaacacc tcaatggcgt gacgctcttt ttccgcttca tgatagctat tgccgattag
    18121 cacgtatttc tgatgctgct gagcgaccag atccaccgct tttaccagcg cgccgaaaaa
    18181 cgcatcagaa acgtccatca ccaccacgcc aatggtgtcg ctaacctgag ttgccagcgc
    18241 ctgggcattg gcgtttggcc gataatccag ctcactcacg gctttcatta ctgcttcacg
    18301 cgtgtcggca ctgaccagcg tgctgttatt gagcacccgg gaaaccgttg ccacagagac
    18361 gccagcctga cgcgctacat cacgaatggt gatcatattc actacctgtt atgagattgc
    18421 agtaaatgac tgcttgctgc cggctatttt gtcagcactg aatacaggac ttcgtgaatc
    18481 gagtcacagc aatggaaacg gttacagccg ttttgttaac gaatgtgatc cagatcgtta
    18541 tcttgatgtt ttagatattg ccggtccggc agcacgcaaa gttaactgac gccagagcca
    18601 ttccaccggc ccctggcgga agtaacgcag ccagataaca gagaagagaa tattcgccag
    18661 ccataccgga ataacaaacg ccagcaattc cagacggtca aactgcataa acagaccgag
    18721 gtggtaaaaa agcgtggtac aaatcagcgt ttgcaataga tagttggtca gcgccatccg
    18781 tccgacgcag gcgatcgcca gcaccagctt aaagcggctc aattgcggcc agaagccata
    18841 aaacagcgac gcatagccaa tcgcctgaaa cggcgcactc agttcccgcg gcatttgaag
    18901 taagaaggca caccagcgat acgcccagtc cagctgccat tgcagggcga tggcaggaag
    18961 gttaatgatc accccaatcg ccactagcac aaaaccagta cgacgatagt gacgtaagct
    19021 gaactgccct ttcagccagc cactgcgcat caatgcggca ccaatgagca tcatcccagc
    19081 cagttgccag ccatactgtg cgcccagtgc caataaactg ttcccaacac catcggcacg
    19141 attactgatc gcttcaacgc cgccgtgaag cttccagtat ttttcatata aaatagccga
    19201 tgcatccggc gtccaggcgc ggctagtctg gctatcggaa atcaacccca ataacagcaa
    19261 aacgccaagc cccaccagat aaagcatgac gccggtatta aaaaggcttt ttaccgatgg
    19321 cgcatcgcgc accagccgcc agcagattaa gcccaccagc ccgtaagcca gcagaatatc
    19381 gccgtcccag aacaataaac cgtgaataaa gcccagcaag actaacagcg ttaaccgcga
    19441 ctggatccag cgtctgccac ggggcagcaa catttgcagg ccagcaccaa acagcagcgc
    19501 aaaaagcgtg aggaatttca cctggccgat gagatcgaga aatgcccagg tccatgcatc
    19561 ctgcggcgta atagcgccgt accaggcggg attaagataa gccgcctttg gtagcccaaa
    19621 ggcgctgatg tttaatagca ggatccccag aatggcgacg ccgcgaacaa agtcgagcgt
    19681 gacgttgcgc tccatggttc ctgcctttaa atcagttgtg atgacgcaca gcgcgcagaa
    19741 actcgtggcg cgtattctga ctggatttga acaatccacc aagagaggtc gttgtcgtgg
    19801 cactggttgc atcgcggatg ccacgcgcct tcacgcaata atgcaccgcg tcgatagaga
    19861 cagccacgtt attggtgccc agcagcgttt gtagcgcaat gagaatttgc tgcgtcagac
    19921 gttcctgcac ctgcggacgc tgggcaaaga actgcacaat acggttaatt tttgacagac
    19981 cgatcaccga atctttcggg atataagcca ccgtcgcttt gccatcgatg gtaacaaaat
    20041 ggtgttcaca ggtactggtc agagtgatat cgcgcacggt gaccatttca tcgaccttca
    20101 ttttgttttc aatgagggtg attttcggga agttggcgta atccagaccg gagaaaattt
    20161 catcgacata cattttagcg atgcgatgcg gcgtttccat caaactgtca tcagccaggt
    20221 cgagattcag cagctgcatg atttcggtca tatgaccagc aataaggctt ttgcgcgttt
    20281 cgttatccat ttcatgcacg ggcgggcgca gcggtgtttc cagtcctcgc gcaactaacg
    20341 cttcatgaac cagggccgct tctttactga gtgatggcat ttatgatttc tcctgcaggt
    20401 gtgacgcctc cgccctgcgt gggggcaaag ttattaagct gatttacagc ctgattattg
    20461 tgcgtgaggc ggcgcacata atccagtatt cacagcgata attattgtaa ttgccgctgc
    20521 ctttcatcag cagatgttaa aacatcgtta tgcaaatacg gaagtgaaag ttactcacag
    20581 cacattgaat aaacggtatg atgaagaaat tgcaaacaac acaacaagga gccacgcatg
    20641 gaaatgctcg aagagcaccg ctgttttgaa ggctggcagc aacgctggcg acacgactcc
    20701 agtaccttaa actgcccgat gacgttcagt atctttctcc caccccctcg tgatcacacc
    20761 ccgccaccag tgctgtactg gctttccgga ttaacctgca atgacgagaa cttcaccacc
    20821 aaggcgggtg cccagcgcgt ggcggcggaa ctggggattg tactggttat gccagacacc
    20881 agcccgcgcg gcgaaaaggt tgccaacgac gatggctacg atttaggcca gggcgcaggc
    20941 ttttatctta atgccacgca accgccgtgg gcgacgcatt accggatgta tgattatctg
    21001 cgtgatgaat taccggcgct ggttcagtcg caatttaatg tcagcgaccg ctgcgctatt
    21061 agcggtcatt caatgggtgg tcacggtgcg ctgattatgg cgctgaaaaa cccgggcaaa
    21121 tacaccagcg tttcggcctt tgcgccaatt gtgaatccgt gcagcgtccc gtggggaatc
    21181 aaagcgttta gccgctattt aggtgaggac aaaaatgcat ggctggaatg ggacagttgc
    21241 gcgctgatgt atgccagtaa cgcgcaggat gcgatcccga cgcttatcga tcagggtgat
    21301 aacgatcagt ttcttgctga ccaactgcaa cctgcagtac ttgcagaagc cgcgcgccag
    21361 aaagcatggc cgatgacgct gcgtattcag ccgggatatg atcacagtta ctacttcatc
    21421 gcctctttta tagaggatca cctgcgcttc catgcgcagt atttactgaa gtgaaagtcc
    21481 gcccggttcg ccgggcatct tctcatcaga agcgataatc cactgccata aagtaacgac
    21541 gtccgtcttc gttatagctg tagtcgtcac gactgagatc cttgtcgcca aggttcagca
    21601 cgcctgcgcg cagtttgacg tctttagtca cctgccaggc cgcgccggta ttccagatgg
    21661 tataaccgcc cggtgtttta gccgtcgcgc tgtcggctcg tttctgcccg gtatagtgac
    21721 cagaaacata gaatgaccag tcttccagcg ccagcggttt ccagtccagc gtaccgttag
    21781 cggtatggaa cggcagatcg gatagcggtt tgttttcgcc gttgctgaca tcacgaccat
    21841 cgttgtaggt gtagttgagt gacagtttcc attcatcgtt gaacggaatt ttcagctcgg
    21901 tttccacgcc ctgaatacgt gctttgttaa cgttgtagta gctaaatacc ggcacaccgt
    21961 tcgctgtaaa accaacaaag ttttggtagc ccggtgcagc attgacgtca gacgtacggc
    22021 tgatgctgat acgatctttc acatcgttac ggaaaacggt aacgctggat tcaacccctt
    22081 ccagccagcc ttcttcaccc atgtagtaaa gccccagctc ccaactttcg ctggtttctg
    22141 gtttcagatc cgggctaccc acaatcttac atgcgccacg gcaggaattg ctcgtccagt
    22201 cagggctaag ttgcaacaga gaaggtgctt taaatgccgt cgcccagccc cctttcaccg
    22261 ttacggtgtc ggtggcgtta taaaccaggt aggcacgcgg actccagtgt tcaccgtagg
    22321 tttcgtgatc gtccatacgc acgccggtcg tcagcgccag cggctcgaag atccgccatt
    22381 catcttccac aaacagcgcg tactggctgg cagacgtttt ggagctggtt cccccggtca
    22441 ggttcaccgc atcgctaagt ttgtcgtgac gccattcacc gccaaccgtg agaaactgat
    22501 taatcgccgt cagcggcaac gtgtatttgc cgtcgaccgt attgctttcg gaagttatcg
    22561 ggctgctgtt gccagggttt ttgttctcga ctttctcacc gtagtatttc agttcgctgg
    22621 tgccgtaatc ccaacgccca ttatggctga cggagtagtt ctggcgttcc aggcggtttt
    22681 tgtccagcga gtcggaatca cgatcctgac ggtcgaaacc gtatccggca gtaaaatcgt
    22741 gattttgatt cggtgtccag gcaaattcga cattgccgtt gcggctggag aatccttcaa
    22801 tacgcggcgt ttctccggta tcggtggtcg ttgagttttg cgggtcatcc ttttcacgtt
    22861 ttgccaggct gccgtaagct ttcattccca gcacaccatc aattaatggt ccactggtaa
    22921 agaactgacc gttataggta tcaccgcgat cgcgatgttc ctgaatggtg gtatcgacgg
    22981 taacggtacc cgaccatttc tgaccgattt ttttggtgat gatattcact acaccgccga
    23041 gcgcatcgga accgtacagc gacgacatcg ggccacggac cacttcaata cgttcgatgg
    23101 aatcgaccgg gatccagttc agatcgaaat cattgtggcg gaagacggca ttgcgggagt
    23161 taacgcgttt accgtcgaca agaatcaggg tgtagctgct gtccagacca cgaatactaa
    23221 cgcccttacg gttatcccct tcgttcgtca gttgtacgcc aggcacttct ttgaggacat
    23281 ccttcagatt ctgtaccggt tttcgctgca ggtcttcctg ggtaatgacg ctgatactgg
    23341 cgggtgcatc tttaaggttt tgttcaacgg aagatgcagt gacaaccatc gtttcgccat
    23401 cgtcatcgac cgctaacaca ggccatgcac aagaaatagc ggacaaacac agcccgaccc
    23461 gtacgaaagg gttcaaccta aacattccat atctccatga ggtaactacg aaaataaaat
    23521 gggttatcgc tcacatcttc ttcacgtccc cttgcgtacg gcagcatcgc ggtggactta
    23581 tttgtatgca ggtgatcatc ccagaaagcc ggaccagcct ctgatttgtc agcctttttt
    23641 gattgcggcg taacgataat gcaaacgata acaattatca atccaaatgt taaattttat
    23701 atccgcatga tttgtcaggg aaatggaatt gaaaaagccc tctcggttga gagggcttag
    23761 caaggaaggg agaaacttat ttcttatcgt tctgcgggaa cttcatttcg ctgtagcgta
    23821 cgaagtgggt tcctttaatc agcttgtagc cgaaccaaat aatcaggaac agcgggatac
    23881 caatatacgt tgccgctacg ccgccccagt caatggtatc tttcaggaac gcttcgtagt
    23941 tctggcccaa agtgataatc agacacagaa tgaatgcgaa gatcggcccc aatgggaaga
    24001 aacctgaacg gtacggcaga tcgttaatgt cgtgtccctg caatacgtaa ccgcgacgga
    24061 agcgatagtg gctaatggca atccccagcc aggcgataaa accggtcatc ccggaggtat
    24121 tcagcagcca caggtatacc gtctggttgc caaacatgga ggtcaggaag cacagaccgg
    24181 caatcaccgt cgtcgcatac agcgcattac gcggcacgcc accacgcgac agtttagcga
    24241 aaatgcgcgg cgctttaccg tcacacgcca gggtgtacag catacgagta gacgcataca
    24301 taccggagtt acccgctgac agcaccgccg tcagaataac tgcgttcatc accgccgcag
    24361 cagagagcag acccgcgtgc tggaacacca gggtgaacgg actaacgctg atgtctttaa
    24421 catcgttacg cagcaggctc ggatcggtgt acggaataat caggctgata atcaggatcg
    24481 cgaacacata gaacaacagg attcgccaga acacctgacg taccgcgcgt ggaatgtttt
    24541 tccccggatc ttcggactcg cctgcagcaa taccgatcag ctcggttccc tggaaagaga
    24601 agccgacaat catagctacg ccgatcatcg ccgcaaaacc accagcaaac ggcgcttcgc
    24661 cgattgtcca gttgctccag cccgcaggct gcgcgccttt gaagataccg ataatcatca
    24721 gcacgccaac gatgataaag acaataactg tcgtgacttt gatcagtgag aaccagtatt
    24781 ccgcttcacc aaagccgcga acagagatgt agttcagcag gaagataacg ccgaggaaca
    24841 acgcactcca gatccagccc ggtgtatccg ggaaccacca gctcatgacc agctgagctg
    24901 caaccaggtc aacggcaata gtcaccgccc agttgtacca gtagttccag cccagcgcga
    24961 agccaaagcc ctcttcaaca tagttctgac cgtaagtggc aaacgaaccg gaaaccggca
    25021 tatatgcagc cagttcaccg agactggtca tcaggaagta aaccatcagg ccaatcaaca
    25081 tatacgagag caatgccccg cccggacctg cctgagaaat cgttgcgcca gaggcaacaa
    25141 aaagacctgt accgatggaa ccgccaatgg caatcatcgt caggtgacgc gcctttaatt
    25201 cacggcgtaa gcccggcgct tctgtggttt tagtttcgga aaccatacaa aaatgctatc
    25261 catctaaaaa atgaggcgcg attgtagcag acgattggca atccttccgg cagaaataca
    25321 cggttataag acaccttcat gatcgcccag ggattataag taaagcagcc aatctctttt
    25381 ctggcgaatg catgctgaag gcgctgcgtc acgaaatcga cacagcacca gcatgttctt
    25441 gtacagcaac ttaacgcggc acatttgcgg gatcgcaata gtccagaaag cgccgtagcg
    25501 cgttggaaag gtgtttttgc cgatgatgta tacgccacaa cgtacgcatc aggcgcggca
    25561 gagggaccgc aacttcactt aatgtgcctg cctgcaattg atcttcaatc acacgtcgcg
    25621 acaggcaact aattcccaac ccatgacgca ccgcatgttt gattgcctcg gagttaccta
    25681 attccatcgc catctcaaac ttcggtaaat gtgacagcaa cagataatcg acaatctccc
    25741 gcgtgccgga accgcgttca cgcaggatcc acggcgcagc ggccagctgt tctaaggtga
    25801 ccggaccacg ggccaacggc gaagtcggcg cggcgaaaac caccagctcg tcttccagcc
    25861 acggttcaga aatgatttca gtgctgtggc acggtccttc aataaagcca atatcaacgc
    25921 ggaaatccag caccgcttgc atcacgtcct ggctattccc aacgctaagt tcaatcggca
    25981 actgcggata atcatggcga taacgggcga taactgcagg cagaatgtag ttaccgatgg
    26041 tactactggc atagatacga atcgcgccgt tgtcttcgcg aaacagttgt tcgatttcaa
    26101 ccgcctgttc aagcaatgcc aacgcacgcg gatagagcag ccgcccgtgt tcattaacaa
    26161 ccagtctttt ccccacgcga tcaaacagtt gcacgccaag ctgcccttcc aggtcggtca
    26221 aggctgcgct cactgctgat tgcgacaacg ccagcatcac cgacgcttgg gtggttgatc
    26281 cacttttcaa tacttctgca aaaacttcca actgccggag ggtgatgtgc atagtcgctt
    26341 accacttata aagattaatt ataaatatat aatcaatttt atttttaaac cagttagtcg
    26401 taaccttatg cccggttaaa ggagagggtt atgacgaata tcaccttaca gaaacaacat
    26461 cgtacactgt ggcattttat tccggggtta gccctgagtg cagttatcac cggggtcgcc
    26521 ctgtggggtg gttccattcc cgcggttgcg ggtgccgggt ttagtgccct caccctcgca
    26581 atcttgttgg ggatggtttt aggcaacacc atctatccgc acatctggaa aagctgtgac
    26641 ggtggtgtgc tgtttgccaa gcaatattta ttacgtctgg gtatcattct ttatggcttc
    26701 cgtctgacgt tctcgcaaat tgccgatgtc ggtatcagtg ggatcatcat tgacgtgctg
    26761 acgctgtcca gtaccttcct gctggcttgc ttcctggggc agaaagtgtt tggtctggat
    26821 aagcacacca gctggttgat cggtgccggt agcagtatct gtggtgctgc cgcggtactg
    26881 gcgactgagc cggtagtgaa agcggaagcc agtaaagtaa ccgtggctgt tgcaaccgtt
    26941 gttatcttcg ggaccgtcgc gattttcctc tacccggcga tatatccgct gatgtctcaa
    27001 tggtttagtc cggaaacttt cggtatctac attggttcta ctgtgcacga agtggcgcag
    27061 gtggtggcgg caggtcatgc catcagcccg gatgcggaaa acgcagcagt tatttccaaa
    27121 atgctgcgcg tgatgatgct ggctccgttc ctcatcctgc tggcggcgcg tgttaaacag
    27181 ctgtctgggg cgaacagcgg cgagaaaagc aaaatcacta ttccgtggtt tgccatcttg
    27241 ttcatcgtag ttgccatctt taactcgttc cacctgttac cgcagagcgt ggtgaacatg
    27301 ctggtaacgc tggatacctt cctgctggca atggcgatgg cggcgctggg tctgaccact
    27361 cacgtcagcg cgctgaaaaa agctggggcg aaacctctgc tgatggcact ggtactgttt
    27421 gcctggctga ttgttggtgg tggtgcgatt aactatgtga ttcaaagcgt catcgcataa
    27481 accactacat cttgctcctg ttaacccgct atcattaccg ttttcctcca gcgggtttaa
    27541 caggagtcct cgcatgaaat acattggagc gcacgttagt gctgctggcg gtctggcaaa
    27601 tgccgcaatt cgcgccgccg aaatcgacgc aaccgcgttt gccttgttca ccaaaaacca
    27661 acgtcagtgg cgtgccgcac cgctcacgac gcaaaccatc gatgaattca aagccgcctg
    27721 tgaaaaatat cactacacat cggcgcaaat tcttccccac gacagttatc tgattaacct
    27781 cggacatccg gtcactgaag ctctggaaaa atcgcgcgat gcctttatag atgaaatgca
    27841 gcgttgcgaa cagctggggc tttctttgct caacttccac cctggcagcc atctgatgca
    27901 gatttcagaa gaggattgcc ttgcgcgtat tgccgaatcc atcaacattg cgctggataa
    27961 aactcaaggt gtgacagcgg tgatagaaaa caccgccggt cagggcagta acttagggtt
    28021 taaattcgaa catctcgcgg cgattatcga cggcgtggaa gataaatccc gcgtcggcgt
    28081 ctgcattgat acctgccatg ctttcgctgc cgggtatgat ttgcgtactc cagccgaatg
    28141 cgagaaaaca ttcgcggatt ttgcccgtac tgtcggcttt aagtatctgc gcgggatgca
    28201 ccttaacgat gcgaaaagca cctttggcag ccgcgttgac cgccatcata gcctcggtga
    28261 aggcaatatc ggtcatgatg cgttccgctg gatcatgcag gacgaccgtt tcgacggcat
    28321 tccgctgatc ctcgaaacca tcaacccgga tatctgggca gaagagatcg cctggctgaa
    28381 agcgcaacaa actgaaaaag cggtagcctg aagatgaata accgggaaaa ggagatcctt
    28441 gcaattttac ggcgtaaccc gctgattcag cagaacgaaa ttgcggacat gctgcaaatc
    28501 agccgttcgc gcgttgcggc gcatattatg gatttaatgc gcaaaggccg gattaaaggc
    28561 aaaggttaca ttctcaccga gcaggaatac tgcgtagtgg tggggacaat caatatggat
    28621 attcgcggga tggcggatat ccgttacccg caatcggctt ctcatcccgg tacaattcat
    28681 tgctcagcgg gcggcgtggg acgcaacatc gcccacaatc tggcgctgtt aggccgtgac
    28741 gtccatttgc tttcagtgat tggcgatgac ttttatggcg aaatgctcct ggaagaaacg
    28801 cgccgcgccg gcgtgaatgt ctccggctgc gttcgtttac atggtcaaag cacatcgacg
    28861 tatctggcaa ttgccaatcg agacgatgaa accgtgctgg cgattaacga tacccatctg
    28921 ctggaacagt tatcgccgca attattgaac gggtcgcgcg atttacttcg tcatgcgggc
    28981 gtggtactgg ctgattgcaa cctgacagcc gaggcgctgg aatgggtctt taccctcgct
    29041 ggtgagatcc cggtgtttgt cgataccgtt tcagaattca aagcgggcaa aatcaaacac
    29101 tggctggcgc atattcacac cctgaaaccc actttaccgg agctggaaat tttatgggga
    29161 caggcgatca ccagcgatgc tgaccgtaat gctgcagtga atgcgttgca tcagcaaggt
    29221 gttcagcaac tgtttgttta tttgcccgat gagtcagttt attgcagcga aaaggatgga
    29281 gaacaatttt tgctgaccgc accagcgcat acgacagtag acagttttgg tgctgacgat
    29341 ggttttatgg cgggcctggt atatagcttt ctggaaggaa acaatttccg tgacagcgcc
    29401 cgttttgcga tggcctgcgc ggcaatttca cgcgccagcg gcagcttaaa caaccctacc
    29461 ctgtctgccg ataacgcgct ttcattagtg ccaatggtgt aacaatgttg ccggattcga
    29521 cgcttgaccg catcttatcc ggctcataaa tcgcacacta cgctaaacca ataaagaacc
    29581 cggcaatggt ggcactcatc aggttagaaa gtgtcgccgc cgccagcgcg cgtaaaccaa
    29641 gctgggcgat ttccggcgca cggtgtggcg caaccgcaga aaacgccccc accaccaccc
    29701 cgatagaacc aaagttagcg aaaccgcaca acgcgaagga aataatcgcc acggttttag
    29761 catccagagt gccagccgtt tgcagatagg gtgagaaatt gagataagcg acaaattcat
    29821 ttattgccag tttctgtcca atcaaactcc cggcaagatt cgcatcactc cagtcaacac
    29881 ccattaccca cgccagtggg gccagcagat aacctacaat ggactccagc gaggcatgtt
    29941 caaaaccaaa ccagccgcca acgccgccga taataccgtt aattaacgca atgatggcga
    30001 cgaatgccat cactactgtc gccacacctg cggcgatttt cagcccggtc attgcccctg
    30061 tcgcggcggc ttcaataatg ctttttggcg gtgtttcggt gaaagagagg ttattaaaag
    30121 aaacctgcga agattccgta gccgggctta gcaggcgggc aaacaagatc cccccgggga
    30181 tcgccattaa cgatgccgcc agcaaatatt ccacaggcac gcccagtgcg gcataaccaa
    30241 tcattgtcga accagcaatc gaggccatgc cgctacaaat cgctgtaaat aattcattgc
    30301 gattcagccg atcgataaag ggcttcacaa tcgccggaat ttcgttttgc ccgaggaaaa
    30361 tggtagtgac cgcgacgaat gactcaatct tgctgatatt taatgctttc tggaatatac
    30421 cgccgagaat gcgaattaaa atccccatca caccgatgta gtagagaata ctcaccagtg
    30481 cagtgacgaa gataattgcc ggtaataccc tgaaaccaaa gataaatcct gcgccatcaa
    30541 ataaggtgtc cattttcggt ccgaccagag aaccgaagat aaatgcgcta cccgcgtcgc
    30601 tgtacgccat cactttatgc acaccaaaag cgactttttc agcgacccaa cgccctggcg
    30661 gtaaccaaag cataatgccg ccaatcacga cctgtaacac taacgccgcg ccaacggtac
    30721 gcaggctgat cttcttcttg tttactgaca gtaaaaacgc aatcgtcagc aataccacca
    30781 ttcccagaac acttctcatg acatccatag tgattatctc ttcatgtcat attgggcgac
    30841 ggctttctgc ccatcagaaa tagccaggcc tgcatcttgc gataccggcc cggcttgtta
    30901 tcaacactta atgggttttg atgtagccgc gcacgcactc ttcgactaag ccccagaacc
    30961 agtcgctatc aatagtgatg ccgactttgg tattagcggg cttgccaaga acgcccagct
    31021 cgtcgcagac ggtacgccca taacaagggc cactgttgac gtccacttcg acgtacatct
    31081 cctgggtttt aatgccatca gggttaatca gataaccgat gcaggtggcg tcgtgcaccg
    31141 ggccgccagc caggccgtag ttttcgaact gggttttgag agtgaagttc atgatgtcgc
    31201 tgaacagctc tccggcaggg ccgcctgccc tttccattcg tgcaatcacg tccggggtgc
    31261 aaacggtctg gttggtgaga tcgaggccca tcatcactaa tggaacgcca gaggtgaaca
    31321 ccacgcgtgc ggcttccggg tcagcaaaga tgttgaactc ggcagatggc gtgaagttgc
    31381 ctgtaccgta agcgccgccc attagcacaa tttcacggat tttgggcagg atcgcgggtt
    31441 gcatacgcat tgccaccgcg atatttgaaa gcggaccaac cggcaccaga gtgatatcgc
    31501 catcgcttgc catcagggta tcaatgatgt atttcaccgc atgagtgctt tctgcctggc
    31561 gggtcagcgg ctcgaatacc gggccatcca gtccggtatc accgtgaata ttatcggcaa
    31621 cgatttgttg ccgcataatg ggttgtggca tccccgcata aaccggaaca ttaatctcca
    31681 gtttctggca aacattcagg ccattaatta atgttttatc aagcgtctga ttacccgcta
    31741 caatagtgat gcctaataaa tctattgccg gatgtttcgc cgccatcatt atagcaatag
    31801 catcatcatg acccggatca caatccagaa taatttttct cttttccatt gtttatttcc
    31861 tctgtttcca gttgcgttat tttttctaca gcaaagaaat ttcgatcggg ccgatgattg
    31921 aatcttaaca acagcgtacg tatgctaaat atgagaaatc tcatagcgga taaacatcgt
    31981 gaaagaaatc cacaataatg atcttaagca gcaattgatg agtgaatccg cgtttaagga
    32041 ttgcttttta acggatgttt cagccgatac gcggctgttt cattttttag cgcgtgacta
    32101 cattgtgcag gaagggcaac agccgtcctg gctgttttac ctgacgcgag gccgcgccag
    32161 gctttacgcc acgctagcta atggtcgcgt gtcgctgatc gatttctttg ccgccccctg
    32221 ttttattggt gagattgagt taatcgataa agaccatgaa ccgcgtgcgg tacaggctat
    32281 tgaagagtgt tggtgccttg cgctccctat gaaacattac cgtccgctgt tattaaacga
    32341 caccctattt ttacgaaaac tttgcgtcac cttaagtcat aaaaattatc gtaatattgt
    32401 ttctttaact cagaatcaat catttccgtt agtgaatcgc ctggcagcat ttatattact
    32461 ctcacaagaa ggtgatcttt atcacgaaaa gcatacgcaa gcggcagagt atttaggcgt
    32521 ttcttatcga catcttttat atgttctcgc gcaattcatt cacgacggtt tattaattaa
    32581 aagcaagaaa ggatatctaa ttaaaaacag aaagcagttg tcagggctgg cactggagat
    32641 ggacccggag aataaattct ccgggatgat gcagtaaaaa ttatttgcaa tagcgcgatt
    32701 gccggatgca acgcttgatg cgttttatcc ggtctacaaa ttgagcatta cgccagacca
    32761 ataaagaacc cggcaatggt cgcgctcatc aggttggaaa gcgttgctgc tgccagcgcc
    32821 cgtaaaccaa gctgagcgat ttccggtgcg cgttttggcg aaatagccga aaatgcccca
    32881 acgacaacac cgatagaacc aaagttagca aaaccacaaa gcgcaaagga gataatcgca
    32941 atggttttca cttccagcgt gccgcccgtt tgcaggtatg gggagaaact caggtaagcg
    33001 acgaattcgt taatcgccag tttctgccca atcaggctac ccgcaaggtt ggcatcactc
    33061 cagtccacac ccatgatcca cgccagcggt gccagcacat agccaaaaat actttccaga
    33121 gaagcattgg cgaagccaaa ccagccgcca attccgccga taatgccgtt gatcagcgca
    33181 ataattgcca caaatgccat taccaccgtc gccacaccag cggcgatttt cagcccggtc
    33241 atcgcaccgc tcgccgctgc ttcgataaag ctttttggcg gcgtttcgct gaacgacaga
    33301 ttttcaaacg tgacctgcga aagctcggtt gctggactaa gaatacgtgc aaacaaaata
    33361 ccgccgggga tcgccatcag cgatgccgcc aacaggtagt caattggtac gcccattccg
    33421 gcataaccaa tcatcatcga accagcaatg gacgccatcc cgctacaaat agccgtaaac
    33481 aactcgtttc gattcatgcg atcgataaac ggtttaacga tcgccgggat ctcattttgc
    33541 ccgaggaaaa tagtggtgac tgcgacaaaa gattcgattt tgctgatgtt gagggctttc
    33601 tggaaaatgc tgccaaggat gcgaatcagc agccccatca cgccaatgta gtacagcaga
    33661 ctaatgagcg cagtgacgaa gataatcgcc ggaagtacgc gaaaggcgaa gataaaaccc
    33721 gcaccgtcaa agaggacatc cattttcggc ccgaccagcg aaccaaaaat gaaggcgcta
    33781 ccagcatcac tgtaagacat cactttatga acgcctaatg ccgcctgttc tacagcccac
    33841 tttcccggcg gaaaatagag catgatgcca ccaagagcaa tttgcagcag tagtgcggct
    33901 gcaaccgtac gcaaactgat gctcttttta ttcactgaca acagaaatgc tattgccagt
    33961 aacaccacca tccccacaac acttctcatt atgtccataa tgattttccc ttcatgccag
    34021 taaacccggc gtcagcgcca ggtttcggta tgtttgatga gtacgggcga cggctttctg
    34081 cccgtcagaa attaacccgc gagacgctga tattctttgg caatttcgct cgccagaatg
    34141 gcgttgttga acacaagctg gatgttggat ttcaggctgt caccgccggt cagctcagca
    34201 acgcgcgcca gcaggaatgg cgtactttct ttaccaataa caccctgcgc ttcagcttca
    34261 gctaccgcct gatcgatcac cgcattgata gtgtgttctg gcatcgcaaa ctgttccggg
    34321 atcgggttcg ctaccacgag gccaccgttc agcccgcttt gccatttcac cgccattgca
    34381 cgggcaattt cactggcgct gtcgagacga atgctgacgt caaacgggct ggtacggcag
    34441 aaaaacgcag gcagcgcttt agtctgatag ccaattaacg gcacaccgaa ggtttctaaa
    34501 tactcagtgg ttaatcctaa atcgagaata gatttcgccc cggcacaaac aacggtgaca
    34561 ttagtatttg ccagttcttg caaatcggca gaaatatcga aggtatgttc cgcccctcga
    34621 tgaacaccac caattccgcc ggtggcaaat actttaattc cggcaagcgc cgcaataatc
    34681 atcgttgaag ccacggtggt tgcaccattt tttcctgcgg caacgacaaa aggtaaatcg
    34741 cgacgactaa ctttagtcac gttatgccct tcacgaccca gtaattcaat ttcttctttg
    34801 cttaaaccca ctttcatcac gccgccaata atggcgatag tggcaggtac tgcgccctgt
    34861 ttacgaatag tttcttctac ttcaattgcg gtctgggcat tttgtgggaa cggcatcccg
    34921 tgagaaataa tggtcgattc cagcgccaca accggttttt tgttttttaa agcgtcctgc
    34981 acttccgggg aaatttttaa ttcagacatt ctgcgttctc cactaacgat ataacgttgg
    35041 caatcgataa atcggggtta ttggtgtatt cacaggagag cgccattgac gaacatccct
    35101 gtgcgaaacg aacagattcg gcaaacggca ttccgtctac ccaacacgaa gcaagtcccg
    35161 ccatcatggc atcgcccgct ccggtaacat taataacatt ggttttgatc ggcgcagacc
    35221 agccactttc accgctgata tcgctgtaat aaacgccgtc gccgcccatg ctcaatacca
    35281 gtcggttcag gccatgttga tggaaccagg cagcaacttt tgccacatct tcacgccctg
    35341 acagcgcaat cccactcagg gtttccgctt caaggcggtt tggcttgaga gtatggattt
    35401 gacttaatcg ctcgcgtact ttgacgcatt tccacgcgga aaccggatcg acaaatacgg
    35461 gtacgttggc agcattatcc agaatccatg ccagtgcctc ttcactgata ttacagtccg
    35521 cgacaatgac ctttgccctc tgaataaatt cacggtgctg tgcgagatat tcagctgtaa
    35581 tagcgttgct aatattcatg tcatttatag caaccagcat ttcaccggta ttatcgagta
    35641 atgataaata gctcgacgta ttttctcccg gcacaatcag gcatttatcg acataaacgc
    35701 cagattgatt ggtttgcgtt agcagcgatt ggccataaaa atcactgcct acggcgctca
    35761 gtagccaggc tttattaccc agcaacgcca ggttttgtgc aatattgcgc cctaccccac
    35821 caggcgtaaa ttttatttta cctggatttg aatccgcata atttaatgat tcatgtgaat
    35881 atccggcgac atcaatattc gccgagccta taattacgac ataatccttt tcgcgcatag
    35941 cacgtcctct ggtaaaaaat taccaaccct gtcaatccac aaggatatag atactgcaaa
    36001 gacggtttaa acatgtgttc atatttaaac atgtgctcat agtatgcatg ttttacgtaa
    36061 agtaaattgc cgccagagag gcaaagcggg cagatttgag caggctcaca aaatgcgatt
    36121 aaatgcctga attacaaaag cgaggaattt gtgacgggat gcacagaaca tgaacactaa
    36181 aaaagggcag aaaagaatct gcccttgagg aaataacctt agtgtttgat gaacgtttca
    36241 ggtttctact gaaagataaa accatttaaa ttcataaaat tacattcaca aaccggtcat
    36301 tctctttgcg ctcatccaat gatacggggc agtaaatttc tgccctgtaa cacacctttt
    36361 attacgcggc tttcgctact gcgtccactt ccggacgttt caggaaggca taggccaaac
    36421 ccgccaccag cgtaccggca ataattgcta ccaggtaacc cagtaccggc gtaatagcgc
    36481 cagggatcag cagaacaaac agaccaccgt gcggtgccat cagtttcgca ccaatcgcca
    36541 ttgagattgc gccagtcagc gccccaccaa cgatacaaca cggcagcaca cgcatcggat
    36601 cacgagcagc aaaaggaatt gcaccttccg aaataaagca cagaccaaga accagagcgg
    36661 ctttgccacc ttcctgctgc gctttgtcaa atttgcgacg cgccaccatt gttgccagac
    36721 ccattgccag cggtggcacc atacctgccg ccataatcgc cgccatcggg ccataggttt
    36781 gagtactcag cagacccaca ccgaatgcgt acgctgcttt atttaccgga ccgcccatgt
    36841 cagtacacat catgccaccg aggatcgccc ccagcagaac cgcattcgca gtccccatgg
    36901 tctgcagcca gtgagtcaac ccttcgagaa tgccagcaac cggtttaccg atcaggtaga
    36961 tcatcgccag accgaccacc agactggaaa ttagcggaat gatcaggatc ggtttcagcg
    37021 cctccatact ctgtggcagt ttcagttgcg tactgattaa cttcgcaatg taaccagcca
    37081 ggaagcccgc aataataccg ccaatgaagc cagaaccggt gctgaccgcc agcataccgc
    37141 caatcagacc cggcgtgaga cccggacgat cggcaatgga gaaggcaata taacctgcca
    37201 gtaccggcac catcagcgca aaggctgaac caccaccaat ctgcatcagc gccgctgcca
    37261 acgtacccgg ctctttaaac gcttcgatac caaaagcaaa agaaagcgcg atacacagac
    37321 cacctgcaac gaccatcggc agcatgtaag agacgcccgt cagcaagtga cggtatgcgc
    37381 ctgcactctc gttcttacct tcagaggtcg ccgtttgagc tttgcccgcc ggttcatacg
    37441 gcgttgcttc agcaaccgct ttatccagtt cctgcgcggt tttcttcagc gccagaccgg
    37501 tagaggtgcg atacatcggt ttaccagcaa atttcgccag atccacttcg atatctgccg
    37561 ccacaatcac cagatccgct gcggctactt cttcgggagt gattgcatta cccgcgccaa
    37621 cagaaccacg ggtttcaact ttcacccacc agccacgttt tttcgcttcg gtttcaatgg
    37681 cttcagccgc cataaaggtg tgtgctacgc cagtcgggca agcagtcacc gcaactacgc
    37741 gtttcggacc gctggcggca accggtgctg tcgcagcgac cggcgcagtg taaggtttcg
    37801 catggccttt ggcttcactc aggaacagct cagggtgcgc aactgcccgg gaaatatcgc
    37861 ccagccagac atttttaccg ttcagcgcgc tgtcattcgg gatggaatca ccgagaacaa
    37921 tcgccatttc tgcgtcgttc ggattgtcga tgatttccag ttttgctttt cgcgccgccg
    37981 cgcccagcag ggtcttcgcc atataggcgc gtgcctgacc gagattagcg tcaataatca
    38041 gcagcgtttt cattatgcct ctcctgctgt cagttaaaag gttgtaagtc gacgcgcgcc
    38101 atcattgcgg ccaactgcgg acgatcggta atacccacat tgctttgact taccgccagg
    38161 gctgcaacag cagtcgccag acgcagtgtg tgttcactgg attcacgcat tagcaagcca
    38221 taaatcaggc caccaaccat agaatcccct gcgccaacgg tgcttacgac atcgactgac
    38281 ggtggtttgg cgatccattc gccggaggca ttaacccaaa gcgcgccttc ggcacccagt
    38341 gaaataacga catgcgcaat accttgttca cgcagcgcat gcgcagcttc aatcacatct
    38401 ttcatttcag gcagtttacg gcctgcccag atttccagct cgcggcggtt aggtttcacc
    38461 agccacggtg ccgctttcaa acctgctact aacgcttcac ggctactatc aaagataatg
    38521 caagggcact gactacgcag gcgagtcatc cagtcggtga acgcttccgg gctgacgcct
    38581 gacggtaagc ttccgctgac acagaccata tcgaactgac cgagccagct cagagaatca
    38641 gtcacaaagc gttcccagtc ggcgggggtg acttcaaaac ccgagaagtt gaagtcggtc
    38701 acttcgccgt ctttttccgt cagcttaacg ttaattcggg tgcgcccctg tacaacctgg
    38761 aaacggttgg caatgcccag ctcgctgaac agttgctgaa aaccatcctg attgtcttta
    38821 ccgaggaagc cgccaacggt gacatcaatt cccaggtctt ttaatacttt ggccacgttg
    38881 atgcctttac ccgccgcatg cagaccggtg gttttcacca ggttcacttc gccgcgttca
    38941 atttccgggc agaaaccaac aaggtcataa gccggattaa gggtgatagt agcaacacgt
    39001 ctgctcatta tgcgccctcc ccaagaccag cagcgatagc gtcgccgatt gctttcagcg
    39061 cctgttcagc atctgcaccc tgggcggtaa agcgtaggcg atgacctttc ttaacgccaa
    39121 gtgccacaac tttcatcaga ctacgtccgt ttgccggttt gccggtgcca tcaaggtttg
    39181 tcacggtaat atcactgtta aattgtttaa tggtgttgac cagcatggta cctggacgag
    39241 catgcaggcc gtgttcattg cgcaccacaa actccgcgct taacacgtcg tcggtcggcg
    39301 catcatcgct ggtcagcagc gccagcaacg ttgccgcatc cgctttcagc aagcggtcag
    39361 ctttattgtc gagcaacaaa tcagcgagac gcttaagaac cgcaatgggc tgatcgtcat
    39421 tcatcgccac actcaccagc atggctgccg tttcgccgtc cacatcaaaa gcatttgccg
    39481 cacggcttac cgcaattgcg ctacgcagat tgccttcggc gctatcgctc agccagatac
    39541 cctgtccgag gttcagcggt tgttcattga tggctttggt gacgaaagtg gcgtcaactg
    39601 cccccgcctc tttcagacgc gcagcgttca gcgcctgaag agtcagcaga tcgctggcga
    39661 cgatatccag cgtcagcatt tcgttgtcga gcttcagctg ctcactctgc ttttcgccca
    39721 tcagcaatgc gcgaagttct tctgctgttg ttgctgactt cagttgttca acaacggaat
    39781 catcgctcag tacgtgggtc agctggcgta gcaggcccag atgctcatcc gagctggcag
    39841 caataccgat cgctacgtac gctacctgac cgtcacccca ggtgacgcct tccgggaact
    39901 gaaatacctg aacgccggtt ttcagcacct gatcgcgggt gtcggtagtg ccgtgtggaa
    39961 tagcaatacc attgccgagg aacgttgagg tttgctgttc gcgcgccagc atgccattga
    40021 cgtagccttc tgctacatta ccggcctgca ccagcgccgc agcgacctgg cgaatcgcct
    40081 cttctttgtc tccggccttt tcgcccggat ggatgtcctg tacggataac tggaacatag
    40141 ttctcctctc ttgctgaatt gaaacgattc agcttctatg agaaaaaaag cgccaacctg
    40201 gcttagggtt aaagacaaga tcgcgctgaa acgtttcaag aaagcataat acttctgttt
    40261 cagcacgcaa ggaaagccgt aaagttatga gcaaaagttt gatgtgctgc acaatttttt
    40321 gtcatttttc ctaattgctg atgggaaaat ccgttatcag caatttcatt tcagcagcgt
    40381 cagcagccaa tcagcaacag tgaaatggca ttttgattat tcgtggcgct tttgatttcg
    40441 ctgtctgttt attttctgac aagcagcgta aactccgcgt cttcctcttc cagtgatcga
    40501 ccagcatgca taactccccc gcagtctcca gcgcgaaatc gtttgacctg acctcgacgg
    40561 cgtttttaat cgttgccttt ctcaccggta ttgcgggcgc tctgcaaacc ccgacactca
    40621 gtatttttct taccgatgaa gtacatgccc gtccggcgat ggtgggattc ttctttaccg
    40681 gcagcgctgt cattggggtt ctggtgagtc agtttctcgc cgggcgctct gataagcgcg
    40741 gcgatcgcaa atcgctgatt gtcttttgct gcctgttagg cgtgctggcc tgcacccttt
    40801 ttgcctggaa tcgcaactac tttgttttgc tattcgttgg cgtctttctt agcagctttg
    40861 gctcgaccgc taacccgcaa atgtttgccc ttgcccgtga acatgccgac aaaaccggac
    40921 gtgaggcggt gatgttcagc tcttttttac gcgctcaggt ttcactggca tgggtcattg
    40981 gcccaccgct ggcttatgcc ttagcgatgg gtttcagctt tacggtaatg tatctgagcg
    41041 cagcggtagc atttattgtt tgcggcgtga tggtgtggct gtttttaccg tcgatgcaaa
    41101 aagagcttcc gctggcgacc ggcacggttg aagcgccgcg ccgtaaccgt cgcgatacgc
    41161 tgctgctgtt tgtcatttgt acattgatgt ggggctcgaa cagcctgtac atcatcaaca
    41221 tgccgctatt tattatcaac gaactccatc ttcccgagaa actggccggt gtgatgatgg
    41281 ggaccgccgc cgggctggaa atcccgacca tgttgattgc cggatatttc gccaaacgtc
    41341 tgggtaagcg tttcttaatg cgcgttgctg ccgtgggtgg cgtctgtttt tacgcaggaa
    41401 tgctgatggc gcattctcct gtcattctgt tgggcttgca gctgctaaat gctattttta
    41461 ttggcattct gggcggtatc gggatgctct attttcagga tctgatgccc ggtcaggcag
    41521 gttcagccac cacgctctat accaacacgt cgcgcgtggg ctggatcatc gcgggatcag
    41581 tggctggcat cgtcgccgag atctggaatt atcacgctgt gttctggttt gcgatggtga
    41641 tgattatcgc cactctgttt tgcttactgc ggattaaaga tgtttaaggc gcggtcagca
    41701 tttccagatc gataagccag gtcattgcct gctgacgcga gttaccgcac atttcagcgc
    41761 tggcttgtaa gcctgcacag actttcggcc gcagtggcga ggtgaaaatt ttacagcgtt
    41821 gctgttcatc aagctgaatg cagggcgtat tggcgggctt gccatctggc ataccgggaa
    41881 tagggctgga aattgaaggg gcagtgcaac acgccccgca acccggacgg cattccatac
    41941 gttgttctct taaggttaac caccggattg atgagcgcgc acagtaccac cttttttgca
    42001 ccagcaaaag tgcgaatacc acttgcccga aaggctcgtc gcgagtactt tgtcgcgata
    42061 tttttgacat tttcgactac aggaattttt cgatgccaag agcgaacgaa attaaaaaag
    42121 gtatggtact gaattacaac ggcaaactgc tgctggttaa ggacattgat attcagtcgc
    42181 ccactgcccg cggtgccgct acgctgtaca aaatgcgttt ttctgatgtc cgtaccgggc
    42241 tgaaagtaga agagcgtttc aaaggagatg atatcgttga caccgtgacg ctgacccgcc
    42301 gttacgttga cttctcctat gtcgatggca acgaatatgt ctttatggat aaagaagact
    42361 ataccccgta taccttcacc aaagatcaga ttgaagaaga gttgctgttt atgccagagg
    42421 gcggcatgcc ggacatgcag gtgctgacct gggacggtca actgctggcg cttgagctgc
    42481 cgcagaccgt tgatctggaa atcgttgaaa ccgcaccagg catcaaaggg gcatctgcca
    42541 gcgcccgtaa caaaccggcg acattgagca ctggtctggt gattcaggta ccagaatact
    42601 taagcccggg cgaaaaaatt cgtatccata tcgaagaacg ccgttatatg gggcgtgctg
    42661 actaacttca gccgcatgca gaaaagggat agctcaggct gtcccttttt taatttatta
    42721 taccaatctt ctattttgcg cttcctgaac accttacgcc ctcaattttc actcgttgat
    42781 tgatcgccct cacacttcat cgcattaaca atccagacca atttcaattg ctgtcatata
    42841 actttacact gtcattgtta attaatcgtt actaagacat gactcctatg aatacaatcg
    42901 cctccgttac gctcccgcat catgtacacg ctccacgcta cgatcgccag cagttgcaat
    42961 cacgtatcgt tcattttggc tttggagcct ttcaccgcgc acatcaggcg ttactgaccg
    43021 atcgtgtgct gaatgcccag ggcggcgact gggggatctg tgaaatcagc ttgttcagcg
    43081 gtgatcaact gatgagccag ctccgcgcac agaaccattt atataccgtg ctggagaaag
    43141 gggcggacgg caatcaggcg ataattgtcg gtgccgttca cgaatgcctg aatgcaaaac
    43201 tggattcctt agcggcaatt attgagaaat tttgcgagcc acaggtggca attgtttccc
    43261 tgacgattac cgaaaaaggc tattgtattg acccggccac cggtgcactc gacaccagca
    43321 atccgcggat tattcacgat ctacaaaccc ctgaagaacc tcactccgca ccgggtattc
    43381 tcgtcgaagc actgaaacgc cgccgtgagc gcggccttac accgtttacc gtgctctcct
    43441 gcgacaatat tcccgacaat ggccatgtgg tgaaaaacgc ggtgctggga atggcagaaa
    43501 aacgttcgcc agaactcgcc gggtggataa aagaacacgt cagttttccg ggaaccatgg
    43561 tcgaccgcat tgttccggct gcaaccgacg aatcactggc agaaatcagc cagcatctgg
    43621 gggtaaatga tccctgcgcg attagctgcg aaccgtttat ccagtgggtg gtggaagata
    43681 acttcgtcgc tgggcgtcct gcctgggaag tcgcaggtgt acaaatggtg aatgatgtcc
    43741 tgccatggga agagatgaaa ctgcggatgc ttaatggcag ccactctttt ctcgcttatc
    43801 tgggttacct ctcaggattc gcccatatca gtgattgcat gcaggatcgc gcatttcgcc
    43861 atgccgccag aacattaatg ctggatgagc aagcgccgac actgcggatt aaagatgtcg
    43921 atttaacaca atatgcggat aagttaattg cacgttttgc taatccggcg ctgaaacata
    43981 agacctggca aatcgcgatg gatggcagcc agaaattacc gcaacgcatg ctggcaggta
    44041 ttcgcataca tctggggcgc gaaacggact ggtcgttgct ggcattaggc gttgcaggct
    44101 ggatgcgtta cgtcagcggc gttgatgatg ccggaaatgc cattgatgtt cgcgatccgc
    44161 ttagcgataa aattcgcgat cttgttgcgg gcagcatcag tgagcaacgc gtcaccgccc
    44221 tgctttccct gcgtgaagtt ttcggtgatg atctgccaga taacccgcat tttgtgcagg
    44281 ccatcgaaca agcccggcaa caaatcgcac aattcggcgc gcatcaggcg ctattaaaca
    44341 ccctcaaaat ttaacgattt ctgcggttaa agcggatgaa gctcgccttc gtccgctctc
    44401 cccttctctt ttctgccgtt tttagccagg attaacgctc agttaactta ccagagtatt
    44461 gcggagctgt tgtgaccagg accaacctca tcaccggttt tctcggcagc gggaaaacca
    44521 cgtcgattct tcatctgtta gcccataaag atcccaacga aaaatgggcg gtactggtta
    44581 atgaatttgg ggaagtcgga attgatggtg ctttgctcgc cgatagcggc gcactgctga
    44641 aagagatccc cggcggctgc atgtgctgcg ttaatggttt acccatgcag gtagggttga
    44701 ataccttact gcgtcaggga aaaccagacc gcttgttgat agagccgact gggctgggcc
    44761 atccgaaaca gatcctcgat ctgttaaccg caccagtcta tgaaccgtgg atagatctgc
    44821 gcgccacgtt gtgcattctc gatccacgcc tgctgctgga cgaaaaaagc gccagcaatg
    44881 aaaacttccg tgaccagctg gctgccgcag acatcattgt cgccaataaa tccgaccgtg
    44941 cgacacctga aagcgagcaa gcgctacagc gctggtggca gcaaaatggt ggcgatcggc
    45001 aattaattca cagtgagcat gggaaagttg ccggtcatct tctggatttg ccgcgtcaca
    45061 atttagccga gttgcccgcc agcgccgcgc attctcatca gcatgtcgtg aaaaaagggt
    45121 tagcagcgtt aagcctgcca gagcatcaac gctggcgtcg cagtctgaac agtggacaag
    45181 gacatcaggc ctgcggctgg atattcgacg ctgatacggt attcgacacc attggcattc
    45241 tggaatgggc gcgacttgca ccggtggaac gcgtcaaagg cgtgctgcgt attcccgaag
    45301 ggctggtgcg aatcaaccgt cagggcgatg acctgcacat tgaaacgcaa aacgttgcgc
    45361 caccggacag ccgtattgag ctgatttcca gcagcgaagc tgactggaat gccttgcaga
    45421 gcgcgctgtt gaagcttcgt ttagcgacta ccgcgtaagg ttgcctgcgt ttttcagtaa
    45481 gataattaga gaaaatatga ttaaaaattt gccgcaaata gtgttgttga atattgtcgg
    45541 cctcgcgctg tttctttcct ggtatattcc cgttaatcat ggattctggt tgccgattga
    45601 tgcggatatt ttttatttct ttaatcagaa actggtcgaa agtaaggcct ttttgtggct
    45661 ggttgcattg accaacaatc gcgctttcga cggttgttca ctgctggcaa tgggaatgtt
    45721 gatgctgagt ttctggctga aagaaaacgc cccaggcaga cgacgtatcg tgattattgg
    45781 tctggtcatg ctattaactg cagtggtatt aaaccagctg ggtcaggcat taattcctgt
    45841 aaaacgggcc agcccaacat tgacttttac cgatattaac cgcgtcagcg aactgctctc
    45901 tgtccccacg aaagatgcct cacgagatag ctttcccggc gatcacggca tgatgctgct
    45961 tattttttcg gcattcatgt ggcgttattt cggcaaagtt gcaggtctta tcgcccttat
    46021 tatttttgtg gtttttgcgt ttcccagagt aatgattggc gcacactggt ttactgacat
    46081 cattgtcggt tcgatgaccg tgatattgat cggtttgccc tgggtgttgc tgacgccatt
    46141 aagtgatcga ttaatcacct tttttgacaa atcactaccg ggaaaaaaca aacatttcca
    46201 aaacaaataa ctcacagtac ttaacatcat caggtttatt tttatagtga ggataatcct
    46261 gatgatgcgc accgtgcttt catccatcga aagcaaaaat cattctctaa gtaaatgaat
    46321 ggattgcatg catttcactc aatcgtactt taattgacca atcccgctta ttaactttct
    46381 gtatcacttt ttcttataaa aaatcatgta aaaccgctcg ccaagaccgc accaatcggg
    46441 taatctcgaa ctcgttttgc ctcggcggta gattatcctc acagcatata attttgtgcg
    46501 ttagtccaca gatttggcct taaggaattg tttcaacatg ccctggtaat tagtctcgtg
    46561 tcgcttggca ttttttttat aacgatattt gtcgttaagg acttcaaggg aaaacaaaca
    46621 acatggtcaa atctcaaccg attttgagat atatcttgcg cgggatcccc gcgattgcag
    46681 tagcggttct gctttctgca tgtagtgcaa ataacaccgc aaagaatatg catcctgaga
    46741 cacgtgcagt gggtagtgaa acatcatcac tgcaagcttc tcaggatgaa tttgaaaacc
    46801 tggttcgtaa tgtcgacgta aaatcgcgaa ttatggatca gtatgctgac tggaaaggcg
    46861 tacgttatcg tctgggcggc agcactaaaa aaggtatcga ttgttctggt ttcgtacagc
    46921 gtacattccg tgagcaattt ggcttagaac ttccgcgttc gacttacgaa cagcaggaaa
    46981 tgggtaaatc tgtttcccgc agtaatttgc gtacgggtga tttagttctg ttccgtgccg
    47041 gttcaacggg acgccatgtc ggtatttata tcggcaacaa tcagtttgtc catgcttcca
    47101 ccagcagtgg tgttattatt tccagcatga atgaaccgta ctggaagaag cgttacaacg
    47161 aagcacgccg ggttctcagc cgcagctaat aaaccgtttg gatgcaatcc cttggctatc
    47221 ctgacgagtt aactgaaagc actgtttagg cagtgctttt ttgttttcat tcatcagaga
    47281 aaatgatgtt tccgcgtctt gatccaggct atagtccggt cattgttatc ttttaaatgt
    47341 tgtcgtaatt tcaggaaatt aacggaatca tgttcatacg cgctcccaat tctggacgta
    47401 agctcctgct tacctgcatt gttgcaggcg taatgattgc gatactggtg agctgccttc
    47461 agtttttagt ggcctggcat aagcacgaag tcaaatacga cacactgatt accgacgtac
    47521 aaaagtatct cgatacctat tttgccgacc tgaaatccac tactgaccgg ctccagccgc
    47581 tgaccttaga tacatgcaag caagctaacc ccgaactgac cgcccgcgcg gcgtttagca
    47641 tgaatgtccg aacgtttgtg ctggtgaaag ataaaaaaac attctgttca tctgcgaccg
    47701 gtgagatgga cattccactc aatgaattga ttcctgcgct cgacattaat aaaaatgtcg
    47761 atatggcgat cttacccggc acgccgatgg tgccgaacaa acccgcaatc gtcatctggt
    47821 atcgcaaccc tttgctgaaa aatagcggcg tctttgccgc tctgaatctc aacctgacgc
    47881 cttctctctt ttatagctca cggcaggaag attacgatgg cgtcgccctc attattggca
    47941 atactgcgct atctaccttt tcttcacgtt tgatgaacgt taacgaatta accgacatgc
    48001 cagtccgtga aactaaaatt gcgggcattc ctctgaccgt tcggctttat gcagatgact
    48061 ggacatggaa cgatgtgtgg tacgcatttt tactgggcgg catgagtgga actgtcgttg
    48121 gcctgctctg ctattacctg atgagcgtac gtatgcgccc cggcagagaa atcatgaccg
    48181 ccatcaaacg cgaacaattt tacgtggcgt atcaaccggt ggtggataca caagctttgc
    48241 gagtaacggg cctggaagta ctgctacgct ggcggcatcc tgtcgcggga gaaattcccc
    48301 cggatgcctt cattaacttt gccgaatcgc aaaagatgat tgtgccgctg actcagcacc
    48361 tgtttgagtt aattgcccgc gatgccgcag aattagaaaa agtgctgccg gtaggcgtca
    48421 aatttggcat taacattgcg ccggaccatc tgcacagcga aagctttaaa gcaaatatcc
    48481 agaaactgct cacttcccta cccgcacacc atttccagat tgtgctggaa attaccgagc
    48541 gcgatatgct gaaagagcaa gaagccacac aactcttcgc ctggctgcac tcggtcggcg
    48601 tagaaattgc tattgatgac ttcggcaccg ggcacagcgc gcttatctat cttgagcgtt
    48661 ttacgctcga ttatctgaaa attgaccgtg gatttatcaa cgccatcggt acggaaacga
    48721 tcacttcacc cgtacttgac gcggtgctga cgctggcgaa acgcctcaat atgctgacgg
    48781 ttgctgaggg ggtcgaaacg ccggaacagg cgcgatggct aagcgaacgc ggcgttaatt
    48841 tcatgcaagg ctactggatt agtcgcccgt taccgctgga cgattttgtc cgctggctga
    48901 agaaaccgta tacgccgcag tggtaaggtg tgcttacgtc ccttattatt catagtgaaa
    48961 gcatgccgga ttgcggctaa tgatgagtaa aaggaaatcc gttgcagatg attgtgcgca
    49021 tactgctgct gtttatcgct ctgttcacct ttggtgcgca ggcgcaggct atcaaggaaa
    49081 gctatgcctt tgccgtgctg ggcgaacccc ggtacgcgtt taatttcaac cattttgatt
    49141 atgtgaaccc cgccgcgcca aaaggtgggc agataacgtt gtcagccctc ggtaccttcg
    49201 ataatttcaa ccgctatgca ctgcgcggca acccgggcgc acgcaccgag cagctgtacg
    49261 acacgctatt tacgacttcc gatgacgaac caggcagtta ttacccgctg attgctgaaa
    49321 gcgcacgcta tgctgacgat tattcctggg tggaggtcgc tattaatcct cgcgcccgtt
    49381 ttcatgatgg ttcgcccatt actgcccgcg atgtagagtt tacttttcaa aaatttatga
    49441 ctgaaggcgt gccgcaattt cgtctggtct acaaaggcac caccgtcaaa gccattgcgc
    49501 cgttaaccgt gcgcattgag ttagctaaac ccggcaaaga agatatgttg agtctgtttt
    49561 cgctgccggt atttccagaa aagtactgga aggatcacaa acttagcgat ccgctcgcca
    49621 cgcctccgct tgccagtggt ccgtaccgaa ttacgtcctg gaaaatgggg caaaatattg
    49681 tctattcccg cgtgaaagat tactgggcag caaacttacc ggtaaaccgt ggacgctgga
    49741 atttcgacac cattcgctac gattattacc tcgatgataa tgtcgccttt gaagcgttta
    49801 aagcaggtgc ctttgatttg cgtatggaaa acgacgccaa aaactgggcc acacgctata
    49861 gcggtaaaaa tttcgataaa aaatacatca tcaaagatga gcaaaagaac gaatcagccc
    49921 aggatacgcg ctggctggcg tttaatatcc aacgtccggt attcagcgat cgccgggtcc
    49981 gggaagcaat caccctcgcc tttgactttg aatggatgaa caaggcgttg ttttacaatg
    50041 cctggagtcg cacgaacagt tattttcaga ataccgaata cgcggccaga aattaccccg
    50101 acgccgcgga gctggtgctt ctggcaccaa tgaaaaaaga tttaccgcca gaagtcttca
    50161 cacaaatcta ccagccgccg gtatcgaaag gcgatggcta cgatcgtgac aacctgttaa
    50221 aagccgacaa acttctcaac gaagcgggct gggtgctgaa aggtcagcaa cgcgttaatg
    50281 ccactacggg tcagccgctc agctttgaat tattgcttcc tgcaagcagc aatagtcagt
    50341 gggtattgcc gttccagcac agcctgcaac ggctgggtat caacatggac attcgcaagg
    50401 tggatcactc gcaaatcacc aaccgcatgc gcagtcgcga ctatgacatg atgccgcgcc
    50461 tatggcgggc gatgccgtgg cccagttccg atttacagat ttcctggtca tcggaatata
    50521 tcaattccac ttataatgcc cccggcgtgc aaagcccggt tatcgactcg ctgatcaacc
    50581 aaattattgc cgcgcaggga aataaagaaa aattactgcc gttagggcga gcactggatc
    50641 gcgtattaac gtggaattat tacatgctgc caatgtggta catggcggaa gaccgtctcg
    50701 cctggtggga taaattctcc caacccgctg tgcgccctgt ttacagtctg ggtatcgata
    50761 cctggtggta tgatgttaat aaagcggcca aactgccgtc agccagacaa cagggagagt
    50821 agatgggcgc ttatctgatt cgccgtctgt tgctggtgat cccaacgtta tgggcgatta
    50881 tcactatcaa ctttttcatc gtgcaaattg cgcctggcgg tccggtcgac caggccatcg
    50941 ccgccattga gtttggtaat gccggagtat tacccggcgc aggcggtgaa ggcgttcgcg
    51001 ccagccatgc gcaaacgggc gtcggtaata tcagcgacag taattaccgt ggcggacgcg
    51061 gattagatcc agaagtgatc gccgagatca ctcatcgcta cggtttcgat aagccgatcc
    51121 acgaacgtta cttcaaaatg ctctgggact acatccgctt tgattttggc gatagcctgt
    51181 ttcgcagcgc ctcggtgctg acgctgatta aagacagtct gccggtttcc atcaccctcg
    51241 gattgtggag cacgctgatt atctatctgg tgtcgattcc gttaggcatt cgcaaagctg
    51301 tttataatgg gagccgcttt gacgtctgga gtagcgcatt tatcatcatc ggctacgcca
    51361 ttccggcctt tttgtttgcc atcctgctga ttgtcttctt cgcaggcggc agctatttcg
    51421 acctgttccc tctacgcggc ctggtttccg ctaactttga ttcgctgccg tggtatcaga
    51481 aaatcaccga ttatctgtgg catatcacgc tgccggtgct ggcgacggtg attggtggct
    51541 ttgcggcgct gactatgctg acaaaaaact cattccttga tgaagtacgc aagcaatacg
    51601 tggtgaccgc gcgtgcgaaa ggggtaagtg aaaaaaatat tctctggaaa catgtgttcc
    51661 gcaacgccat gctgctggtg attgccggtt ttccggcgac gtttatcagc atgtttttta
    51721 ccggctcgct gctgattgag gtgatgtttt cactcaatgg tcttggctta ctgggctacg
    51781 aagcgaccgt ctcgcgcgat tatcctgtaa tgtttggtac cttgtatatt ttcaccctga
    51841 ttggcctgct gctgaatatt gtcagtgata tcagctatac gctggtcgat ccgcgtatag
    51901 attttgaggg acgttaatgt cgcgactcaa ccccgtcaat caggcccgtt gggcgcgttt
    51961 tcgccataac cgtcgcggct actggtcgtt atggattttc ctcgttttgt ttggtttgag
    52021 tttgtgttct gaacttatcg ccaacgataa accgttgctg gtgcgttatg acggcagttg
    52081 gtatttcccg ttgttgaaaa actacagcga aagcgatttt ggcggcccgc tggcaagtca
    52141 ggctgattat caggacccgt ggctgaaaca acggctggaa aataatggct gggtactgtg
    52201 ggcaccgatt cgctttggtg ctaccagtat caactttgct accgataagc ccttcccttc
    52261 tcccccctcc cggcaaaact ggctgggaac ggatgccaac ggcggcgatg tgctggcacg
    52321 tattctctat ggcacgcgga tctcggttct gtttggcctg atgctgactc tctgttccag
    52381 cgtgatgggc gtgctggcgg gggcgctaca aggctattac ggcggtaagg tcgatctctg
    52441 ggggcagcgc tttattgaag tatggtcggg gatgccaacg ctgtttttga ttattctgct
    52501 ttccagcgtt gtgcaaccta acttctggtg gctgctggca attactgtct tatttggctg
    52561 gatgagtctg gtcggcgtgg tgcgggcgga gtttttacgt acccgcaatt tcgactacat
    52621 ccgcgcggca caggcgcttg gcgtcagcga tcgcagtatt attctgcgtc atatgttgcc
    52681 gaatgcaatg gtcgctaccc tcaccttttt accgtttatt ttatgtagtt cgatcaccac
    52741 cctgacctca ctcgatttcc tcggcttcgg tctgccgctc ggttcaccgt cactcggtga
    52801 actgctgtta caagggaaaa ataaccttca ggctccgtgg cttgggatca ccgccttctt
    52861 gtcggtggcg atattattgt ctttgctgat ctttattggt gaagccgtcc gcgacgcatt
    52921 tgatcctaat aaggcggtgt agcatgacgc aaactctgtt agcgattgaa gatttgtcgg
    52981 tgggttttcg ccatcagcaa accgtacgta cagtagtcaa tgatgtttca ctacagattg
    53041 aggctggcga aacgctggcg ctggtgggtg agtcaggttc aggcaaaagc gttaccgcgc
    53101 tgtcaatttt acgcctgctc ccttccccgc cggttgaata tctctccggc gatattcgtt
    53161 ttcatggcga atcgctgctt catgccagcg accaaacgtt gcgcggtgta cgcggtaata
    53221 agatcgccat gatttttcaa gagccgatgg tgtcgttaaa tccattgcat accctggaaa
    53281 aacagcttta tgaagtgctt tcactccacc gcgggatgcg tcgggaagcg gctcgtggcg
    53341 aaattcttaa ctgccttgat cgcgttggta tccgccaggc ggcaaaacgg ctgacagatt
    53401 atccgcatca gctctccggc ggcgaacgcc agcgggtgat gattgcgatg gcgctgttaa
    53461 cgcgaccgga attattaatt gccgatgaac cgaccaccgc actggacgtc tctgtccagg
    53521 cgcagatttt acagctgttg cgcgaactgc aaggcgagtt gaatatgggc atgctgttta
    53581 ttactcataa cctcagcatt gtcagaaaac tggcccaccg cgtggcggta atgcaaaacg
    53641 gtcgctgtgt cgagcaaaat tacgccgcta cgctatttgc ctcacccact catccttaca
    53701 cacaaaagct actcaacagt gaaccgtcag gcgatccagt gccgttgcca gaacctgcct
    53761 caacgttgct ggatgttgaa cagcttcagg ttgccttccc cattcgcaaa gggattttga
    53821 agcgcattgt ggatcataat gtggtggtga aaaacatcag ttttacgcta cgagcgggtg
    53881 aaacactggg tttagtgggc gagtccggtt ccgggaaaag cacgacggga ctggcgctgc
    53941 tgcgactgat taattctcag ggcagcatca tctttgacgg tcagccactg caaaatttaa
    54001 atcgccgcca gctgttacct attcgtcatc gcattcaggt ggtatttcag gatccaaact
    54061 cctcgctcaa cccacgactc aacgttttgc agattattga ggaaggctta cgggttcacc
    54121 agccgacgct ttctgccgca caacgcgaac aacaagtgat agacgtgatg catgaagtgg
    54181 gattagatcc tgaaacacgc caccgttatc cggcggagtt ctctggtggt cagcgacaac
    54241 gtattgcgat tgccagggca ttaattctta agccctcgct gatcatactt gatgaaccga
    54301 catcatcact cgacaaaacg gtacaggcgc aaatattgac gctattgaaa tcattgcaac
    54361 aaaagcatca actggcctat ttgtttatca gccacgattt gcacgttgtc cgcgcgttat
    54421 gtcatcaggt tatcgtactg cgacaagggg aagtagtgga acaaggaccg tgcgcgcgcg
    54481 tgtttgccac accgcagcag gagtatacgc gtcagctact ggcgttgagc tgacgcttaa
    54541 aaaggattgt agtttgaaaa gggttcggca attgccacac caaaattttt caatcggcat
    54601 gttgcagcaa actcgtcctg gcgatttaca aacaggcacg gctcaccttc acactccagc
    54661 accgaacatg gaacttcgat gtcgcctaac gcctggctta gtttatacat caccgaccat
    54721 gcattatcac catccgggct aagcaattta agcgccacca ggctgttgtc accgcacggt
    54781 ccattttgcg gaatcggttc aaccttcgaa cctgcgaaac cagcagacca gcgatagttc
    54841 tgcggcagtc gatggacgat tgagagttgt aatgaagtca cttattttcc ccggaagcac
    54901 atttacttca caatttgttt acatcaattt taacacatca tcaacaatcc gtctttatac
    54961 agatcgtaaa gattgatgcc tcgcctgcgt ggcctctatg gctctgattt aagtaatatc
    55021 tgcgggcgtg gtcacgtttg cggggctata tgtacccgtt tctgcgatct aactcaacct
    55081 ttttaactac aatgatgtga ctttttacat aaattgattt tacataaaat aaacatatat
    55141 cgggggaatg atagatttgt ggttgacatg catcaacaaa ggaagccttt tagcttcctc
    55201 gttgtgcaat agatcaccgt tttttcggcc gactggcgta cagacagaag agaatggagc
    55261 tggttgcgca gaatgcaatt gaccaaatca tcggccatgc agagttaaag gtcgcaagag
    55321 aaagcaatgc gccaacaatt gcccctatgc caaaacggaa ggttcctgcc agcgaagatg
    55381 ccgttcccgc catatgggga aactcatcaa gaatgaccgc catcgcattg gatgacacca
    55441 tcgacacgca gcccacaaac gccgcaacgc caaccaccag cgaccaaaat cccagaccca
    55501 gcagcgcact gatgaccatc cacgctgcca taataaattg tatccacaac cccgagcgga
    55561 acatatttaa cgcgccaata cggcggacga agcggctatt aaagatggtc atcacgaaca
    55621 ggaaaacaat gtttagtgca aagtaataac caaagttttc cggcgcgacg tggttaattt
    55681 caatgtagac aaacggtccg gcgcttaaga acgagaacat cccggcaaag ctgaaaccac
    55741 tggccagcat ataactcagg acacgcttat gacggaacag cgccgcaaag ttaccaatag
    55801 tggtacgaat gtgaaaaggc tggcgacgct ccggtggtaa ggtttctttg atcagaaaga
    55861 agatcattgc cgaagccaga atcgccgcca cggcgaggat ccagaagatg taatgccagc
    55921 tcagccacac cagcacccag ccgccaacta tcggtgccat cagcggtgca atggtcgtca
    55981 ccagcatgac aaacgacatc atccgcgaga actcttcttt cgggtagata tcacgcatca
    56041 aagcgttgat aaccacgctg gccgcagccg ccgccagccc gtggaagaaa cgcatcacaa
    56101 tcagctgatc gatggtttgt gccaacgcac acgccaccgc ggcggcggca aacaccagcg
    56161 taccgccaag aaccaccggc ttacgcccga agctgtctgc catcggcccg tagattaact
    56221 gccccaacgc aaagcccaga atataagtac tgagggtcat ctgcgtactg cccgccggta
    56281 cgccaaactg cgctgaaatt accggtagcg cgggcagata catatcaatc gacagcggca
    56341 tcaacatggc cagcaggcca aggataaaaa caatagcaaa cgacgaatgc tgtcgggtgg
    56401 tcacaacggg ctcctgaaag tcattgaaaa gttagacgac gctggcaatt tcttcttcag
    56461 ttaacggacg atattcaccg ggggctaaat cagcatccag cgtaatgccg ccaatacgtt
    56521 cacgatgcag ctcaaccaca tggttaccca cggcggcgaa catgcgtttc acctgatgat
    56581 aacgcccttc gctgatggtc agacgaacct gcgttggggt aatcacttca agcaccgctg
    56641 gcttagtgag atctttttcg ttatgcagct gcacgccttt agcaaattgc tctgccgtat
    56701 cgtcagctac gggtgattcc agcgtcacca gataggtctt ctcgcaatga tggcgcggag
    56761 aagtaatgcg gtgcgaccac tgaccatcat ctgtcattag taccagaccg gtggtatcga
    56821 tatccagccg ccctgcggcg tgcagtttcc acgccactgg ttcatctaga aaatagagca
    56881 ccgttgggtg atcagggtcg tccgtggagc aaacatagcc ctgaggctta ttgagcatga
    56941 agtaacgtgg accgtgttgc tgcgccagcg ggttgccatc gtaagcgaca tcatgttcag
    57001 gaagcagttt gaacgctgca ttacggacga tttcgccatc gacggtgaca cgattgccgc
    57061 ggatttcacg cccggcaata gcacggctaa cgccgagttg ctgtgcgata aatttatcaa
    57121 gtcgcatgtg tgtgattttg cctgtaaaaa cggaggtcgg gcattgaccc gaaaatctga
    57181 actgttgtct gcccagtata gcggtctaat aacttccctc aagggaaaaa gaaccgtggc
    57241 atactattag cagaataatc tacctacgcg agaccatgat ttttacactc cgcccatatc
    57301 agcaagaagc cgtggatgcc acgctcaacc attttcgtcg tcataaaacc cctgccgtta
    57361 tcgtgctgcc caccggtgca ggtaaaagcc tggtgatagc ggaacttgcg cggctggctc
    57421 gtggtcgcgt gctggtgctg gcacacgtta aagaactggt ggcgcaaaac catgcaaagt
    57481 atcaggcgct ggggctggaa gccgatattt ttgccgccgg gctaaagcgc aaagagagcc
    57541 acggtaaagt ggtatttggc agcgtgcagt cggtcgcccg taatcttgat gcctttcagg
    57601 gtgaattttc gctgttgatt gtcgatgaat gtcaccgtat tggtgacgat gaagagagcc
    57661 agtatcagca aatcctcact cacctgacaa aagtgaatcc ccacttacgc ctgctggggc
    57721 tgactgccac gccttttcga ctgggcaaag gctggatcta ccagttccat tatcacggca
    57781 tggtacgcgg cgatgagaaa gcccttttcc gtgactgcat ttatgaactg ccgctgcgtt
    57841 atatgattaa acacggctat ctgacgccgc cagaacgact ggatatgcca gtagtgcaat
    57901 acgatttcag ccgcttgcag gcacagagta acgggctatt cagcgaagcc gatctcaacc
    57961 gcgagctaaa aaaacaacaa cgtattaccc cgcacatcat cagccagatt atggagtttg
    58021 ctgcaacgcg caaaggggtg atgattttcg ccgccaccgt tgaacacgca aaagagattg
    58081 tgggattact acccgccgaa gatgctgcac tgattactgg cgacaccccc ggcgctgagc
    58141 gcgatgtgtt aattgaagat tttaaagccc agcgttttcg ctatctggtc aacgtcgcgg
    58201 tactgaccac cggatttgac gccccgcacg tcgatcttat cgccattctg cgccctaccg
    58261 aatcagtgag tctttaccaa caaattgtcg ggcgcggtct gcgtctcgct cctggcaaga
    58321 ctgattgctt aattcttgat tatgcgggta atcctcacga tctctacgcg ccggaagttg
    58381 gtacaccgaa aggcaaaagt gacaacgttc cggtacaggt tttctgccct gcctgcggtt
    58441 ttgccaacac cttttggggg aaaacgaccg ccgacgggac attgattgaa cactttggtc
    58501 gccgctgtca gggatggttt gaagatgacg acggtcatcg cgaacagtgt gacttccgtt
    58561 tccgttttaa aaattgcccg caatgtaacg cagaaaatga tattgccgct cgccgctgcc
    58621 gcgaatgtga cacggtactg gttgatccgg atgatatgtt aaaagcggcg ctacgactga
    58681 aagatgcgct ggtattacgc tgtagcggca tgtctttgca gcatgggcac gacgagaaag
    58741 gcgaatggtt gaaaatcacc tattacgatg aagacggcgc ggatgtgagt gagcgtttcc
    58801 gtctgcaaac gcccgcccag cgaactgcct tcgagcagct ttttatccgc ccgcatacgc
    58861 gcacaccggg catccctctg cgctggatca ccgccgccga tatcctcgcc cagcaagcct
    58921 tattgcgaca cccggatttt gtcgttgccc gcatgaaagg tcagtactgg caagtgcgtg
    58981 aaaaagtgtt cgattacgaa ggtcgttttc gtcgggcgca cgaattacgc ggttaatggt
    59041 agttttgatt gatgtataag gcgattgagt atagaatctc gcccgctttt gcatacgcaa
    59101 agcagatcac ttacctgttg ctgggtcgcc tgtagcagga ttaatttaag agagaaagaa
    59161 atgtttacta tcaacgcaga agtacgtaaa gagcagggta agggtgcgag ccgccgcctg
    59221 cgtgccgcta acaagttccc ggcaatcatc tacggtggca aagaagcacc gctggctatc
    59281 gagctggatc acgacaaagt catgaacatt caagctaaag ctgaattcta cagcgaagtt
    59341 ctgaccatcg ttgttgacgg taaagaaatc aaagttaaag ctcaggacgt acagcgtcac
    59401 ccgtacaaac cgaagctgca gcacatcgac ttcgttcgcg cttaattgct gaataagttg
    59461 taaaaaaccc cgctccggcg gggttttttg tatctgcaga ttatgcctga ttacgatatt
    59521 gctatttttt gcaggccaga taaggcgttc acgccgcatc tggcattcat aacacaaaac
    59581 cgattaattg ccgccggacg tccggcgctg caattgatcg cgcaaattcg gcggcgtgcc
    59641 tttaatagtc agggtatcgg tcgccggatc ccagaaaatc cgttcgccca acagcatcgc
    59701 atcaaaatta atagtcaagc caccgccgct accggcaaac ttcgtcaact gacgcagtgt
    59761 gctacggtct gccgggaagc tctcttccag ttcgtagcct ttttcagccg caaactccgt
    59821 gaagctcact tcgctgacgc ctgccagctc tttcgacagc gattccagtt cgatctcttc
    59881 gcctgcctgc agctgctcat tacagtagct ataaacctgc tggcgcacgt tctgtcgctc
    59941 tgctttatcc agttgtgctt cggcagtgaa atcatcaacc gcttgcaaca gcccacggtt
    60001 ttgcgctttc gcgttcaatc cttcgctggc cccgaggaaa tccataaaga aatccgccac
    60061 tttgcgccct actcgccctt tcaggaaagt gagatagcgg gtcgactctg gattggtttc
    60121 ccattcggtt aaatcgatac gcgcaacaat atccgcatga ttgatatcaa gataatgggt
    60181 tgggttgatg tcgagatttt cgttaacgcg catactgctc aggttgctca ggactgccac
    60241 cagcagatac tccaccgcca gataacggta gtggcaaaac agcacgaatc caccgtcagc
    60301 aaaaggatat ttcgctaatt cgtcacgcag gcgaccggtt gccgcgcggc taaatgccag
    60361 aaagtcctct tcaccctgac gctgtaagcg cagcgtctgc gccagttcac tctcttcgct
    60421 aaacaagccg taagctttat ttttggcgct atagacccga tgcagttctg ctaccatctc
    60481 aacgacggtt tctgtcggtt ccagcaatga atcgcgcaac accagctcaa ggttctgctc
    60541 atcacgcttg ataagctggt gcagagcaat ctggttgata tccagactca tgataaactc
    60601 tcctttaaga ccgggcggta ttcaaccacc gccggttaag cgacgcaata aaagatggcg
    60661 gtcattttac agaaggcggc gtaacgagaa tgatttaacg cataaaaaag cagaaaaaaa
    60721 accgttgcta cggtaatatg ttgccctttc atcaactaac tgatttcgat ttatgccaca
    60781 aatttcccgc tacagtgatg aacaggttga acaattgctc gcagagctgc tcaacgtact
    60841 ggaaaaacat aaggctccga ccgacctttc cctgatggtg ttaggcaaca tggtcactaa
    60901 ccttatcaac accagcattg ccccggccca acgccaggca attgccaact cttttgcccg
    60961 cgccttacag tcctctatca acgaagacaa agcgcactaa gggaaacaga taacaggtta
    61021 tggtaactca tcgtcagcgc taccgtgaaa aagtctccca gatggtcagt tgggggcact
    61081 ggtttgcact gttcaatatt ctgctttcgc tcgtcattgg cagccgttac ctgtttatcg
    61141 ccgactggcc gaccacgctt gctggtcgca tttattccta cgtaagcatt atcggccatt
    61201 tcagcttcct ggtgttcgcc acctacttgc tgattctctt cccgctgacc tttatcgtcg
    61261 gctcccagag gctgatgagg tttttgtccg tcattctggc aacggcggga atgacgctat
    61321 tactgatcga tagcgaagtc tttactcgtt tccatctcca tcttaatccc atcgtctggc
    61381 aactggttat taacccagac gaaaatgaga tggcgcgcga ctggcagctg atgttcatca
    61441 gcgtgccggt tattttattg cttgaactgg tgtttgcgac gtggagctgg caaaagctgc
    61501 gcagcctgac gcgtcgtcgg cgcttcgcgc gtccgctggc cgcattctta tttatcgcct
    61561 ttatcgcctc gcatgtggtg tatatctggg ccgatgccaa cttctatcgc cctatcacca
    61621 tgcagcgcgc taacctgccg ctttcgtacc cgatgacggc gcgacgtttt cttgagaagc
    61681 atggcctgct tgatgcgcag gagtatcaac gccgtcttat tgagcaaggt aatccagacg
    61741 ccgtttccgt tcagtatccg ttaagcgaac tgcgctatcg cgatatgggc accgggcaga
    61801 atgtcttgtt gattactgtc gatggcctga actactcacg cttcgagaag cagatgcctg
    61861 cgctggcagg ttttgctgag caaaatattt cgttcacgcg ccatatgagc tccggcaaca
    61921 ctacagacaa cggcatcttt ggcctgttct atggcatctc gccgagctat atggacggca
    61981 ttctgtcgac ccgtacgcct gcggcattaa ttactgcgct taatcagcaa ggctatcagc
    62041 tggggttatt ctcatcagat ggctttacca gcccgctgta tcgccaggca ttgttgtcag
    62101 atttctcgat gccgagcgta cgcacccaat ccgacgagca gaccgccacg cagtggatca
    62161 actggctggg acgctacgca caagaagata accgctggtt ctcgtgggtc tctttcaatg
    62221 gtactaacat tgacgacagc aatcagcagg catttgcacg gaaatatagc cgggcggcag
    62281 gcaatgtcga tgaccagatc aaccgcgtgc tcaatgcact gcgtgattct ggcaaactgg
    62341 acaatacggt ggtgattatc actgccggtc ggggtattcc actgagcgaa gaggaagaaa
    62401 cctttgactg gtcccacggt catctgcagg tgccattagt gattcactgg ccaggcacgc
    62461 cggcgcagcg tattaatgcg ctgactgatc ataccgatct gatgacgacg ctgatgcaac
    62521 gcctgctaca tgtcagcaca cctgccagcg aatattcgca aggtcaggat ttgttcaacc
    62581 ctcaacgccg tcattactgg gttaccgcag cggataacga tacgctggca attaccaccc
    62641 cgaaaaagac gctggtgctg aacaataacg gtaaataccg cacttacaac ttacgtggtg
    62701 aaagagtgaa agatgaaaaa ccacagttaa gtttgttatt gcaagtactg acagacgaga
    62761 agcgttttat cgctaactga ttaattataa atcagttagc gaaatatctt acttgcaatc
    62821 ggtgtggaaa acggtagtat taacaaccac gagtcggcac gtagcgcagc ctggtagcgc
    62881 accgtcatgg ggtgtcgggg gtcggaggtt caaatcctct cgtgccgacc aaaaatccca
    62941 aaaaaaccaa ccgtaaaggt tggttttttt atatctgcat ttaattcgat aaacagaccg
    63001 cgacacatca gggcctgttt attgtctgtt atcagaacgt ccagaccaca cccgcctggg
    63061 tattccacgg cgattctacc cctgcgccat tgccatagct gactgacaaa tgaccgctta
    63121 acgtcggggt aaacgatgac cttatcccag cctgataaac gccacgggtt ccagccacat
    63181 cattattaaa tttgccatcg tcattaactt tcacctggtt agaatcggcg tattcctgac
    63241 gcacggcggc tttcagccag ggttccagcg tcgtaccgtt ttgcaggtcc atgtgatagc
    63301 ttaccgccgt tcccgcttca gcgcgtaata tccgggtatt tcccacatcc gcgcgcatgc
    63361 cgtttgataa cgtgtagtcc tgaccatctg tggtaaagcc ggtaaaggcc agatagggtc
    63421 taacactcca caatccgtca acccaacgga acccgctctc gacatgagca cccgcgccgt
    63481 tactattgta atcgccaaac gctgttgccc cattactcat cttgccatgg atggtgttgg
    63541 caaaacggtc aactttcacc actccatcaa cataggcacc gttctgatgc tcccaaccgg
    63601 cataagcccc cagggtatag ctatcgacat tgcctttgcc gccgcgatca aaaccaatat
    63661 cagaatgaga gtaaccaaag aacaagccgc gaattgtgct gctttcttca cgggagaaac
    63721 ggctatcgat accgagcgtc aggcccgtca atgtttgctc aaaaccagct cccgcatcag
    63781 tggtcacgtt gttgcgggtg ttaattgccg aactccacat cgccgtatcg taactaacgc
    63841 cttttacgct accaagacgc tcacgcacgg tgtccagttc tgcatcaaat accagcggtt
    63901 gtgcggccgc catattcagc acatcagtgg ttgaaggggt aatttgcgcg cgattctctg
    63961 ccagactcca gctatggtta ccattatcca gcaaggtata ttcatacgta ccgatatcaa
    64021 caacgcctcc ggcattgccc aacgtaaatg cagcatcacc gccgcccgtt gttaccagtg
    64081 taaggctatc tcctgctgcc gggctggcac cggtgtccgt cacgaatatt ttgaaatcac
    64141 ctgttgcctg accggtgacg ttgagctgat cgctctgatg attcgccata tcggtacgca
    64201 tataaaaatt gccgtttccc gacagggtat tggtggtcaa cgtagtgaaa ttttccgcac
    64261 ttgccgtcac agatgaacga gtcactgact catccatcat aatcacattt gcgccagaca
    64321 tcgcaaggtt ggctaagtta aggcgggtgt tttggttcgt cgtagtgata tcggcaatga
    64381 tggtccatgc gctattttcg agcgaaacat ccgctttgct ggtatccacg gcaccatgcg
    64441 ttcttaaaga agcgccctca gaaaccacta ccttacccac caacattgcg ggtgcataat
    64501 ttatttgcgg cttcataacc tcaagaatgc catcatttcc tctgactgac acgttaacca
    64561 ttgtgccagc ccagacgtta gcgcggccat tgttgataac catattttca gagatggctt
    64621 tagaggaata cgttatactt ccattctgat ctgatcgacc gagatcatat accgcatttg
    64681 cctgcactct cgtaccagta tcctttccca gcgattgcat agtggcatgc tcatcgagga
    64741 tagtgtcaat ggcctgcgtg tcctccataa caataagccc ggaaccatca tccagttcat
    64801 aattttttga cacaccatct tttatactaa attggccttt actgtttgta ccactcactt
    64861 ccagcgcatt cgttgagaca attaattttc cgcctgcttt tttatcaacg ccagttgcag
    64921 tcccgccatc cataacctcc agtaagccgc cactatctac agtagtatta taagcgaagt
    64981 cattttcttc aactcgcaaa cttccgccgt tttccaacag catattgtta gcaataccat
    65041 tcttgatttc gaactgaccg agacggtttg ttccgaatac ctccatgacc cgcgtgcttg
    65101 ctttaatagc accgcctgct ttctgatcta ccgcaatcgc taatcccccc tctttaacct
    65161 ccagtaatcc tcccttttcg atagcggcaa ggacggtaat cccatctttt tcaacgatta
    65221 agcgcccgcc tttaatcgtg ggatcattcg ctgcgccata aacctgtagc acaccattat
    65281 tgagcgtagt ctggtttgcc gcgccaccca tttgaacaat ttgataacca tcatttaata
    65341 ctgagtattc tgcggtgcca ttcatttcaa tgtactgata cccgccgtta atttcagtat
    65401 tactacttat accaaattct ttaatatgct gttctccgcc aggattgatt ttcgtattat
    65461 tggcggtccc ataaaccagc tggatatctt tttccaggac aacaccatca actgtctcac
    65521 catactcaac tgttgatgcc aatgctatgg gtgaatataa cgctgtagct accagaacag
    65581 atattgcgga acgacaaaga gaaacagaac cagattgatg cattgagctt tcatcctatg
    65641 aaattaattg cagttaaaag cattgggtac agaaaatacc catagctcca tacccggagt
    65701 cagtttttaa aaactgttta aagaaatgca caagtattgt gattgatttt ttagttgttt
    65761 ttcttgatga gaagctgatg caaaattccg tctttataat gaaaatgatg ccaaagcgaa
    65821 cgacaaggtt gtagttttcg ctacatgtcc atacataaaa tggggtaaca ttcacgcgcc
    65881 tggtagcgtt accaacgcta cgctcaaaca taatgattct aataaaacct caggagacta
    65941 ctatgcctga agcaacgcct tttcaggtga tgattgtgga tgatcatcca cttatgcgac
    66001 gcggtgttcg tcagttactg gagcttgatc ctggctttga agtggtcgcc gaagcgggcg
    66061 acggcgcgag tgctatcgat ctggcgaata gactggatat cgacgtgatc ttgctggatc
    66121 tcaatatgaa aggtatgagt ggcctggata ccctcaatgc cttgcgcagg gatggcgtta
    66181 ccgcgcaaat tattatcctg accgtatcag atgcctccag cgatgtcttt gcactgatag
    66241 acgcaggcgc agacggttat ctgttgaaag acagcgaccc ggaagtgttg ctggaagcga
    66301 ttcgtgccgg agcgaaaggc agcaaagtct ttagcgaacg cgtcaatcag tacttacatg
    66361 aacgtgaaat gtttggcgcg gaagaagatc ccttcagcgt gctgacggag cgcgagctgg
    66421 atgttctgca cgagctggca caggggctgt caaataaaca gattgcctcg gtgttgaata
    66481 tttccgagca gacagtaaaa gtacatattc gcaatctact gcgcaaactc aatgtccgct
    66541 cacgcgtggc agccaccatt ctgttcctgc aacaacgcgg ggcacaataa aaatagcccg
    66601 atggcatcac catcgggcct ctttttattt actctcctgc ggcgacaaat gttgcatcgc
    66661 ctgcgcgata ctacgttcaa tcaccgcacg gcgagtatcg ttggcaggta agagtttcaa
    66721 catcatctcc cacgcggcaa cggcttcgcc aaatcgctgc tgctcaaagg cattaaacgc
    66781 atacatgctt aacacacgaa tattgctatg gtccgttctc accaactggc gcagcagttc
    66841 gccgccgagg cggttgtcgt tgggatcaga tgaacgtgtc aacgcttcag cataacccag
    66901 tgtggcatcg ctgtttttcg gatcgaggcg atacgcagta gcgtatgcat cggtggcgat
    66961 actggcgtta cccagcgcca tgccaacgcg acccaacata atccaacctt ctatatctcc
    67021 cggatttttt tgcagttgag tacgcatccc cagcgcaagg cgcgacatct cttcttcgtt
    67081 gagcggatcg gctttcggat ccagcgccct gtccagcaac gccggagcct gtgccgtggc
    67141 ctgctgccag attttcacct gctgataatt gccagtctgg tagtagctga cgccagccac
    67201 aattaacgcc accacaatac ccggcagata aacaacatat ccggcacgct taccttccgg
    67261 cacgctttgt tcaggaaacg cttccggtac cacgcgtacc cgacgccgcg aacgggcgta
    67321 tatgacccaa ccgccaatac caatagccac taccggaagc acccacagca gcacggtcag
    67381 cggcgttaac ggcggatcgt aagtgacgaa gttgccgtaa cgcgccacca tataatcgac
    67441 aatctctttc ttacttttgc cttcctgcat cagttcatac actttctggc gcaggtcggt
    67501 ggcaatcatc gagttggaat cggcaatgct gttattctgg catttcgggc agcgcagttc
    67561 ttcagtgagc tgacggaact gttgttcctg tgcttcatct ttaaactgca acacgtcgat
    67621 ggtcgccagc gctgagccgg agatcatcag catcagcacg cccaataaaa acctcattgt
    67681 gcggcctcct tactgtattt ctcccacagc ggcttgatct cttcttccca gacgcgagga
    67741 ttgagatcgc ccgcatggcg atagcgaatg atgccgttgc cgtcaataag aaacgtttct
    67801 ggcgcgccat agacaccgag atccagccct aacatgccat cgccatcaaa aaggcttagc
    67861 gcgtaaggat tgcccagctc tttcagccag ctgattgcct tctggcgatc gtctttatag
    67921 ttcatgccga ccacgcggat gccctgcgca gaaagctgat tcagatattg atgttccgca
    67981 cggcaggtcg gacaccaggt cgcccagacg ttaagcagca ctggtttgcc ctgagtcagc
    68041 acatccgcct gataaaactg ccccggattg tccagtgatt cgagacgaaa cttcggcaca
    68101 ggcttgccaa tgagcgccga ttccagattg gtcggatcat ccccttcggc attacgcgcc
    68161 agctgccaca gcagcgccgc ggcaatcgcc aggaagataa tcaacggaat taacaatact
    68221 ttgcgcttca tacagcctcc ggcgcagttt tttgcgggct cacgcgctta cgatagcgag
    68281 gatcaaacag acacagcagt ccgcccaacg ccatcatcag cccgcccgcc caaatccagc
    68341 gaacaaatgg tttgtagtaa agacgcacag cccacgcgcc gttttccagc tcttcaccga
    68401 gcgcggcgta caggtcacgc gtgatgccgc cgtcaattgc cgcttcggtc atcatcgacc
    68461 cggcagtgtt gtaataacgt ttttccgcat acagcaccgt ttccggttta ccctctcgcg
    68521 ttacgccgat agtcgccaca ccgccacgcc agttcgggcc agtcacctct ttgacatcac
    68581 ggaaggtgaa gcgatattca tgaatatcga cgctatcgcc ggacttcatg cgcacatcac
    68641 gctcaacgct atagttctgg ctaaaggcaa tgccaacaat tgtcactgcc agccccaggt
    68701 gagccgccac catcccccaa taactgaagg tggttttcgt gccgcgtgaa atacgtagcg
    68761 cagcttccgc aattgccagc accgcaatcc agcaggccat tgccaggccg agcaccgtca
    68821 tcgccacaac tttgctttcg aacagccacg gcaacagcag agacagcacc agcgtagaga
    68881 tgaaggcgat aatcaataaa ttgcggattt tacgtgggcg atcgcgcccc cagcgcacca
    68941 gaggaccgac acccagcagt agcgcaaacg gcaccatcag ccaggtaaac atggtgttga
    69001 agaacggttc gccaatcgaa atactgccca gtcccagttg cttgtgcacc agcggcagca
    69061 gcgtccccag caacaccacc agcatcgcgg cgaccagcaa aacgttgttc gctaacagca
    69121 aagattcccg cgaccacagc gcattgttta cgcgtgagcg aactttgtgt ccacgcgcgg
    69181 caaacagcag cagcgaaccg ccaatcacca gcaccataaa ggcgaggata aacataccgc
    69241 gcgccggatc agacgcaaac gcgtgtaccg ataccagcac gccggaacgc acgaggaaag
    69301 tccccaacag acacaacgag aaggcactga ttgccagcag taatgtccac gctttgaagc
    69361 tggcgcgttg ttcagtgacc gccagtgagt gcatcagcgc agtccccacc agccacggca
    69421 taaacgaggc gttttctacc ggatcccaga accaccagcc accccagccg agttcgtaat
    69481 aggcccatgc ggaaccgagc acgatgccga gcgtcaggaa gatccacgct gccagcgtcc
    69541 acggacgagt aaaacgcgca taagtgctgt ccagacgccc gctcagcaaa gaagcaatgg
    69601 caaaagcaaa cgccaccgag aaacccacgt accccatata aagcagaggc ggatggaaga
    69661 tcagccccgg atcctgtaac agcgggttaa gatcgcgccc ttcaatcggg aagttcggca
    69721 gcgtgcgtga gaatgggtta gaggtaaaga gaatgaacag caaaaagccg acactgacca
    69781 tccccattat cgccagtacg cgggccacaa tatccagcgg aatacgctga ctaaaaatcg
    69841 ccaccgcaaa ggtccagccg ctcatcagca gcacccacag cagcagtgag ccttcatgtg
    69901 cgccccaggt agccgccacg cgataccaca ccggaagctg ggtattggag ttgctggcaa
    69961 cataggtgac ggtaaagtcg ttgaccacga aagcattgac cagcaccaga aatgcgccag
    70021 ccacagacat aaacagcagc caggcaaaca agcgggaaga cgccatcatg cgcgcatctc
    70081 cgcgcgccac gccccatagc ggatacacgg acagcagcag cgcaattccc agcgccaggc
    70141 acagcagccc gttaccaatt tccggcatca tgatgctggg tccttataaa cactcgccgg
    70201 gcgacggtgg ttagcttcca tcgctttctc aacttctggc ggcgtgtagt tttcatcatg
    70261 tttcgccagc acttcttttg cgaggatatg attgcctttt tccagctcgc cctgcaccac
    70321 aacgccctgc ccttcacgga acagatccgg caaaatgcct tcgtaagaga catccactga
    70381 gccttcagcg tcgtaaatgg tgaaggtcac tttcagcgaa ttgggatcgc gctgcacact
    70441 acccggcatc accatcccgc caacgcgcag acgctgaccg acttccggca tctgctgagt
    70501 ttcacgcttg ccgtagagaa tttcccccgg cgtataaaag agatcgatat tcgagcgcag
    70561 cgcatatagc accagaccga tagtcagcgc cagccctgcc aacacggcac aggcaatcca
    70621 caagcggttt ttacggcgaa tattcatgca gcctcctgct gttgcgcagc acgtaaacgc
    70681 gcctcacgcg cccgctgttg cgccacacca cgcagaattg cgcgatgctg catcaccgag
    70741 tgcacgacca aaaccaccag cggaataacg gtcatcacca ccgccagcca gacaaaaaag
    70801 gcgtaaccgc ccattgcgaa aaattcattc caggaagcaa atgcaggggt catttacggc
    70861 ctcttttcag tatcagttca ctcacccacg gacggcgttt ttccatcagc aaaatcaaat
    70921 tacgcatccg catcagcgtc agcgtggcag agaggagcag gaagccaaaa atcgaccagc
    70981 gcagcggcga acgcatcgcc ggatcgatac tttgctgcat ccgcgttgat ccctgatgca
    71041 gggtgttcca ccactccacg gagtaatgaa taatcggcag attcaccacg ccaatcagca
    71101 ccaggatacc tgccgcacgg cccgccagac ggcggtcgtc gaaggcgtgc cacagggcaa
    71161 tcacacccac atacaaaaac agcagcacca gttcagaagt cagacgtgca tcccataccc
    71221 accaggtgcc ccacatcggt tttccccatg cagagccggt aaccagggca ataaaggtaa
    71281 acacggcacc aatgggggcc atcgccgcca ccgccaggtt ggccattttc atctgccaga
    71341 caaggccaat aaacgccgcc accgccattg atgcataaat gcccatcgac cagatcgccg
    71401 caggcacatg caggtagata atgcggtagc tatttccctg ctgataatca gccggagcaa
    71461 agccgaatcc ccagatccag ccgacggtaa gcacgactac actggcaatt gccagccacg
    71521 gtataaacca gccacagatt tgatacagcc gtggtgggat cgccagttga tgcagtgttt
    71581 tccacatagt ttcgatacca gactcgaaca aaaatcagta atccagcgtt attgaatgct
    71641 gattcgtaac gctgccgccg tcgcaaaagg acttaatgtc gcggtgcctg ccagcaacgc
    71701 gcctaaaatt gccagatacc cgtcaacggg caaatgcata gaagccgcgt ccatcgcggc
    71761 ggtggcaaag atgagtaatg ggatagtcag cggtaacacc agtatgctga gcagcacacc
    71821 accgcgctta agtcccactg tcagcgccac gcccggtgca ccgagaaagc caagcgtagg
    71881 cgttcccagc agtagcgtta acgccatcac ttgccagcca taaacatcca ttcccagtag
    71941 cattgctacc agtggcgaaa ggatgagtaa cggcagaccg gttaccatcc agtgcgccat
    72001 caccttcgcc agcacaacgg cgggcaaggg taacggcaac aacatcaatt gttcaagact
    72061 gccgtcctgc aaatcgtcac ggaacagtcg ttccagcgcc agcaaggatg aaagcagcgc
    72121 ggcaacccag ataatgcccg gtgcaatacg tgccagcagt tgcggctccg gaccgatact
    72181 gagcggaaaa agggtaatta caatcaggaa gaaccacagc gggttggcga tttcagcgct
    72241 atggcgaaac gctacgcgca gctcaagacg gaaaatgcgc cagaacatca tacggccctc
    72301 gtttgcgtca gtgaaatgcg gcgaatttta ctttcagcaa cgttgagcgg ctggtgggta
    72361 gtgagaatca caatcccccc ctgctctgta tgctgcgcca tacgctgggt cagacgatcg
    72421 acgccgttaa cgtcaatcgc ggtaaaaggc tcgtcgagga tccataacgt ggcacgggtc
    72481 agccacagac gcgctaaagc gacgcggcgt tgttgcccgg cagaaagttg atttaccgga
    72541 atatcttcga atccggcaag cccggcctgc gccagggctt ccagacattg tgcggtatcg
    72601 ccatcgcgat gataaaagtg cagattttct aacgccgtca gccgggtttt gatccccggc
    72661 tgatggccta tccataacag gttttgatgg tagctgtcgc gtacctgatg caagggctgc
    72721 ccttgccaga gaacctcgcc tgcgtcaggg cgagacaacc ccgtcagcaa acggagaagc
    72781 gttgtcttcc ccgcgccgtt gctaccggtg atttgtaccc actctcctgc gttcagcgta
    72841 aatgacaagc cactaaataa ggttcgttca tcccgctcac aaagtaactc tctgacttca
    72901 agcataccca cgcaataacc ctgttaaaaa cctggctcga cttcacgcat atcgggcagc
    72961 ttgtgcgcta tccctttatg gcaatcaata caggtttgcc catctttcac cgcctggtca
    73021 tgcatcttcg cggcaaccga tttctgggcg gttgtatcca tatactcgaa gttgtgacag
    73081 ttacggcact cctgcgagtt attgtccttc atgcgccgcc actcattctg tgccatcgtc
    73141 agacgatgag cttcaaattt ctgcggcgtg tcaataacgc caaaaatttt accatacagc
    73201 tctttacttg ctttgagctt gcgtatcatc ttcggcacaa actcgtgcgg aacgtgacaa
    73261 tccggacagg tcgcacggac gccgctacgg ttgttgtagt gcacggaatc catgtattcc
    73321 tgatacaccg tgttacgcat ttcgtggcag ctaatgcaga actcttcggt attggctttt
    73381 tccatcccag tgttaaagcc gccccagaag acgatgccgc cgacaaaacc gatcaacagc
    73441 agcgtcccca gcgccagacg gctgggggta cgccaccatt tccacaggcg cttaatcaga
    73501 ccaggcttac ggtcagaatt tcccataata acctcttatt tcccgtaacc ttttgatggg
    73561 gtaaaggtat tccccacgat tggcgcggta tcggcctgcg gtacgtgaca ttgcagacag
    73621 aaataacgac gcggagccac ttccgcgccc actttgccgt cgctgtccat aaagtgagta
    73681 ggactgatac gcggcgcgcc agtggtgcga tagctttcga caccgtggca ctgcaagcag
    73741 cggttggtat tggtcgttac ctgataacct tcaacgctat gcgggatcat tggcggctga
    73801 ttcacatagt tcagcggcat ccgatcctgc tcttttggca tccgaatggc cccttcctgc
    73861 gtcccggata cttccggcga ctggctaaaa tcgacgccat tagccgccca aaccgcgccg
    73921 cttaccacca gggccagcat cgccgtccat tgacacagcg ctttcttcag gtcatggctt
    73981 ttcatgattt cgctcccgaa ctccatcgtg tagttattgt aaatacatcc tcagaacaga
    74041 catccacgca gcgaccgcac gtcatgcaat cgcggctggt gacctgcacc gggctttgtt
    74101 catccagcac cggggcacgt agcacatgcg gttccgggca aacatgaaaa caatccatac
    74161 agcggttaca tttctgccga tcgctggccg caacggtaat cacacctttg ctacccagca
    74221 cgccatacag cgcgccaacc ggacaaatgt gcccgcacca gccgtgttca acgaccagta
    74281 gatcaaataa aaacagcgcg agaataagca gcgcgccgct gccgaagccc attaccaggc
    74341 tacgccccat cagggaaacg gggttgatcc attcccagat gagcgtgcca gttaaggctg
    74401 atcccaccag aatgaccacc aacagcacgt agcgaatgtg gcgagggatc gtcgcagact
    74461 gattgaggtc aaagcgcctg cgtaaccagt tcgctaagtc ggtaatcggg ttcagcgggc
    74521 agacccagct gcaaaataat cgctttcctg ccagggcgta gagcacggtg ataatcaccg
    74581 caccggtcaa cgccaccgtg gcaggcagat gaccactggc aagactttgc agcgtcatca
    74641 gcggatcggt cagcggaacg gtgtcgaata acaggctact gctatagttg ccgtgcaaga
    74701 tccacacacc aaaccacgga ccgctcaaaa acatccccag cacgaagaac tgacaaaggc
    74761 gacgtaacac cagccaacgg tgactgcgcc accagccttt tttctccagc gcctcgcgcc
    74821 cggcgtcacg tttacgattt gccattgttc ccctccagcc agccgaagcg gtaatggtga
    74881 cctaactccc ctttcgccag tgacagcggt aacaccttga ttgccggttg ttccagcacg
    74941 catacttttt cgcacttacc gcaaccggta caggcgtcgc tgtgaaccgt cggcagaaag
    75001 cgggcgtgct taccggtacg cgtgttgcgc tccagctcca gggtgatggc ctcatcaatt
    75061 ttcgggcatt cgcgataaca aacatcgcag cgcagcccct gaaagttgag acagttttcc
    75121 tggtccacca gtaccaccag ccccatccgc gcgtcgtcga tcgattcaat ctcacgatcc
    75181 agcgcaccgc tcgggcacac tttggcgcac ggaatgtcct cacacatttc gcaaggaata
    75241 tcccgtgcga caaaatatgg cgtgcccgcc gacaggccag aggccagcgt cgccagtttt
    75301 aaggtgtcgt aagggcaagc ctgaacacac tgaccgcaac gcacacaggc actggcaaag
    75361 gcgttctcgt ttatggctcc gggcgggcgc aaccgcacgc cagatgcgcg tgcggtttgc
    75421 tgttgtaacc ccagcgccac acccacggca gccagcccgc ctgctgtgcg aacgacatcg
    75481 cgcagaaagc ggcggcgacc attttgaggt ttcgctgacc gggacataat ggcttacacc
    75541 ttctccagtt tgaccgcgca cttcttgaag tccgtctctt tcgagagcgg atcggtcgcg
    75601 tccagcgtta gtttgttaac cagctgtgcg gcgtcgaaga acggcatgta caccaggccc
    75661 tgcggcggac ggttacgacc gcgcgtttcg acaatcgaga tcacttcgcc acggcgagaa
    75721 accactttca ctttgtcgcc acggcgcaga tcgcgcgctt tcgcatccag cgggtgaata
    75781 aacaggaccg cttccgggaa ggcacggtgc agttccggca cacggcgagt catactgccg
    75841 gtgtgccagt gctccaggac gcgtccggta gagagccaca ggtcgtactc ttcatccggt
    75901 gcttccgccg ccggttcgaa cggcagcgcg aagatcaccg ctttaccatc cggtttaccg
    75961 tagaacttat agccttcgcc cgctttcacg tacgggtcgt taccttcgct gtaacgccac
    76021 tgcgtttctt taccgttcac caccggccag cgcagaccgc gcgctttgtg gtagtcatcg
    76081 aacggcgcga gatcgtgacc gtgaccgcga ccaaaccatg cgtactcttc gaacagccct
    76141 ttttgcagat agaagcccag ctcgcgggat tcatcgttca gctgatcttc cgccagttcg
    76201 gataccggga atttgctcac ttcgggggtg gcatacagaa cttcgtacag cgttttgcca
    76261 cgcagttccg gtttcttcgc cagcagctct tccggccata cttcttcagt tttgaagcgg
    76321 cgggagaact ggactaactg ccagaggtcc gatttcgctt cgcctggcgc ctgtacctgc
    76381 tgacgccaga actgagtacg gcgttcggcg ttaccgtaag ccccctcttt ttctacccac
    76441 attgcggtcg gcaggatcaa gtcggcggcc agcgcactga ctgtcggata cggatcggag
    76501 acgatgatga agttgcgcgg atcgcgccag cccggcatac gctcttcatt aatgttcggc
    76561 ccggcctgca tgttgttggt acacatggtc cagtaaacat tcagcttgcc gtctttcagc
    76621 gcacggtctt gtgctaccgc atgcagaccg attttcgccg gaatggtgcc gctcgggata
    76681 ttccacttct tctcgcagat atcacgatgt ttctcgttag ttaccaccat gtccgcaggc
    76741 agacggtgag caaaggtgcc cacttcacgc gcagtaccac acgcggaagg ctgcccggtc
    76801 agggagaacg gaccgcaacc cggctgggaa attttgccgg tcagcaggtg caggttgtag
    76861 accaggttgt tggcccacac gccacgagta tgctggttga agcccatcgt ccagtaggag
    76921 atgactttct tgttcggatc ggcatacagc tgcgccagtt gttccagctg atcttttggt
    76981 acgccagtca tttcagcggt tttttccagc gtatactcag caacgaaggc tttgtaatct
    77041 tcaaagctca tcggttcgga ggcgtcagaa cccggattct tcgctgcttt ttccagcgga
    77101 tgggtcggac gtaaaccgta gccgatgtcc gtcgcccctt tgcgcaggtt aacgtgcttg
    77161 ctgaagaagt cctgatttat cgcattgttt tgaatgatat agttggcgat gtagttcagg
    77221 atcaccaggt cagattgcgg cgtaaagatg atgccgttat ccgccagctc gaagctacga
    77281 tgctggtagg ttgaaagcac cgccacggtg acgtcctggt tagagagacg acggttagtg
    77341 atgcgtgacc agaggatcgg gtgcatctcc gccatgtttg cgccccacag cacaaacgcg
    77401 tcagcctgct cgatgtcgtc atagcagccc atcggctcat ccataccaaa ggttcgcata
    77461 aagccaacta ctgccgacgc catacagtga cgcgcattcg ggtcgatgtt gttcgaacgg
    77521 aagcccgctt taaacagctt ggacgcggca taaccttccc agatagtcca ctgaccagaa
    77581 ccgaacatac cgatcgattc cggccctttt tctttcaggg cggttttgaa tttctcttcc
    77641 atcacatcga aggcctgatc ccaggtgatt ggggtaaatt cgccttcttt gtcatattta
    77701 ccgtttttca tacgcagcag cggctgcgtc aaacggtctt taccgtacat gatcttgggc
    77761 aggaaatagc ccttaatgca gttcaggcca cggttaaccg gtgcgtccgg gtcgccctga
    77821 caggccacca cacgcccctg ctgcgttccg accagaacgc cgcaaccagt accgcagaaa
    77881 cggcacggcg ctttatccca tttaatggct tcctgctgac caacaacggc gcgggcaacg
    77941 cccggcacgc tgagaccggc agccgccgca gcggccgcaa cggcgttagc tttcataaag
    78001 ctacgacgac tgagtttcat ggtgtttcct caccttgctc ttcctgctgg tgataaacca
    78061 gcgacaccgc cagcacgccc tctacgttgc gtactgactc aatggtttgg atcagcgttt
    78121 cgctgtcttc tgcttccacc accacaatca actgaccgct cggcgcgtcg ctgacagcaa
    78181 cttcacagcc gggaaaggcg ttcagttggg tgctgatgtc tgaaattcgt tcgcttttgg
    78241 cctgcacaac caggctgcaa acttgccagt tagtgtgcat ggagatactc cgcagttatg
    78301 gctgataccg gacagctggc tgcacatgct ccacatccgt tgcaaagttg gctattaagt
    78361 tgcggctggt agatcccgga gagcgtcggg cgaaagataa ttgccattgg ctcacagcta
    78421 tcctgacagc ggcggcattc aactgactga tacgccaggc acgcgtcccc aatggtgaac
    78481 tgcaaatccc aggccctggt gtggcgcgga gaaaatagcg attcggggca agcctgcgcg
    78541 caggcgtaac agaagctgca ctcgttattt ttgaaattaa cgctcggata gccgcccgcg
    78601 ccgcgttgca gaatgttgtt ttcacaagca ttaatacagg cgtcgcagcg ggtgcaatgg
    78661 gtcagaaaat gagattcatc accggaccag ggcggacgga taccgttact ggctttgcgc
    78721 cagcgaccag tgagtatgcc ccgacgggat gcatcaatct tcacattgac cttccatcat
    78781 taacgcgctc tgaaaattga gagcgaccaa ataaaccgca taattaataa gccattttta
    78841 tagccgctaa gatattaaag gatgtgtcaa agatgcatac cccgatcgag gtaaaacctg
    78901 tagcaggatc aaaagagtgg cgggaagcgt ggcaaaaacg ggcttttgct cacatttcaa
    78961 atg
//
LOCUS       DAZKIP010000012        78203 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZKIP010000012 DAZKIP010000000
VERSION     DAZKIP010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 78203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.012"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..1144
                     /gene="proV"
                     /locus_tag="ACHOUK_000942"
     CDS             <1..1144
                     /gene="proV"
                     /locus_tag="ACHOUK_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFP9896446.1"
                     /translation="AFKYIEQGLSKEQILEKTGLSLGVKDASLAIEEGEIFVIMGLSG
                     SGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELREVRRKKIAMVFQSFALMPHMT
                     VLDNTAFGMELAGINAEERREKALDALRQVGLENYAHSYPNELSGGMRQRVGLARALA
                     INPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTIVFISHDLDEAMRIGDRIAIM
                     QNGEVVQVGTPDEIINNPANDYVRTFFRGVDISQVFSAKDIARRTPNGLIRKTPGFGP
                     RSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALTQQQGLDAALIDAPLAVDAQT
                     PLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRALDREGVNNG"
     gene            1137..2201
                     /gene="proW"
                     /locus_tag="ACHOUK_000943"
     CDS             1137..2201
                     /gene="proW"
                     /locus_tag="ACHOUK_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFP9896447.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            2258..3250
                     /gene="proX"
                     /locus_tag="ACHOUK_000944"
     CDS             2258..3250
                     /gene="proX"
                     /locus_tag="ACHOUK_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFP9896448.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            3442..4626
                     /locus_tag="ACHOUK_000945"
     CDS             3442..4626
                     /locus_tag="ACHOUK_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9896449.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISNKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            4750..5487
                     /gene="ygaZ"
                     /locus_tag="ACHOUK_000946"
     CDS             4750..5487
                     /gene="ygaZ"
                     /locus_tag="ACHOUK_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFP9896450.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            5477..5812
                     /gene="ygaH"
                     /locus_tag="ACHOUK_000947"
     CDS             5477..5812
                     /gene="ygaH"
                     /locus_tag="ACHOUK_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFP9896451.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            5903..6433
                     /gene="emrR"
                     /locus_tag="ACHOUK_000948"
     CDS             5903..6433
                     /gene="emrR"
                     /locus_tag="ACHOUK_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFP9896452.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            6560..7732
                     /gene="emrA"
                     /locus_tag="ACHOUK_000949"
     CDS             6560..7732
                     /gene="emrA"
                     /locus_tag="ACHOUK_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFP9896453.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQQMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            7749..9287
                     /gene="emrB"
                     /locus_tag="ACHOUK_000950"
     CDS             7749..9287
                     /gene="emrB"
                     /locus_tag="ACHOUK_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFP9896454.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(9351..9866)
                     /gene="luxS"
                     /locus_tag="ACHOUK_000951"
     CDS             complement(9351..9866)
                     /gene="luxS"
                     /locus_tag="ACHOUK_000951"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFP9896455.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     VWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(10016..11572)
                     /gene="gshA"
                     /locus_tag="ACHOUK_000952"
     CDS             complement(10016..11572)
                     /gene="gshA"
                     /locus_tag="ACHOUK_000952"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFP9896456.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIDKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(11645..12073)
                     /gene="yqaA"
                     /locus_tag="ACHOUK_000953"
     CDS             complement(11645..12073)
                     /gene="yqaA"
                     /locus_tag="ACHOUK_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFP9896457.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFCPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(12070..12636)
                     /gene="yqaB"
                     /locus_tag="ACHOUK_000954"
     CDS             complement(12070..12636)
                     /gene="yqaB"
                     /locus_tag="ACHOUK_000954"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFP9896458.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(12917..12993)
                     /locus_tag="ACHOUK_000955"
     tRNA            complement(12917..12993)
                     /locus_tag="ACHOUK_000955"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12957..12959),aa:Arg,seq:acg)
     gene            complement(13096..13172)
                     /locus_tag="ACHOUK_000956"
     tRNA            complement(13096..13172)
                     /locus_tag="ACHOUK_000956"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13136..13138),aa:Arg,seq:acg)
     gene            complement(13235..13311)
                     /locus_tag="ACHOUK_000957"
     tRNA            complement(13235..13311)
                     /locus_tag="ACHOUK_000957"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13275..13277),aa:Arg,seq:acg)
     gene            complement(13376..13452)
                     /locus_tag="ACHOUK_000958"
     tRNA            complement(13376..13452)
                     /locus_tag="ACHOUK_000958"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13416..13418),aa:Arg,seq:acg)
     gene            complement(13456..13548)
                     /locus_tag="ACHOUK_000959"
     tRNA            complement(13456..13548)
                     /locus_tag="ACHOUK_000959"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13512..13514),aa:Ser,seq:gct)
     gene            complement(13864..14049)
                     /gene="csrA"
                     /locus_tag="ACHOUK_000960"
     CDS             complement(13864..14049)
                     /gene="csrA"
                     /locus_tag="ACHOUK_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFP9896459.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(14284..16914)
                     /gene="alaS"
                     /locus_tag="ACHOUK_000961"
     CDS             complement(14284..16914)
                     /gene="alaS"
                     /locus_tag="ACHOUK_000961"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFP9896460.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFVVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(17042..17542)
                     /gene="recX"
                     /locus_tag="ACHOUK_000962"
     CDS             complement(17042..17542)
                     /gene="recX"
                     /locus_tag="ACHOUK_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFP9896461.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(17610..18671)
                     /gene="recA"
                     /locus_tag="ACHOUK_000963"
     CDS             complement(17610..18671)
                     /gene="recA"
                     /locus_tag="ACHOUK_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFP9896462.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(18751..19248)
                     /gene="pncC"
                     /locus_tag="ACHOUK_000964"
     CDS             complement(18751..19248)
                     /gene="pncC"
                     /locus_tag="ACHOUK_000964"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFP9896463.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITLRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(19393..20478)
                     /gene="mltB"
                     /locus_tag="ACHOUK_000965"
     CDS             complement(19393..20478)
                     /gene="mltB"
                     /locus_tag="ACHOUK_000965"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFP9896464.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDRMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            20733..21295
                     /gene="srlA"
                     /locus_tag="ACHOUK_000966"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             20733..21295
                     /gene="srlA"
                     /locus_tag="ACHOUK_000966"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: protein motif:HMM:NF015565.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            21292..22253
                     /gene="srlE"
                     /locus_tag="ACHOUK_000967"
                     /gene_synonym="gutE"
                     /pseudo
     CDS             21292..22253
                     /gene="srlE"
                     /locus_tag="ACHOUK_000967"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
     gene            22264..22635
                     /gene="srlB"
                     /locus_tag="ACHOUK_000968"
                     /gene_synonym="gutB"
     CDS             22264..22635
                     /gene="srlB"
                     /locus_tag="ACHOUK_000968"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFP9896465.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            22639..23418
                     /gene="srlD"
                     /locus_tag="ACHOUK_000969"
     CDS             22639..23418
                     /gene="srlD"
                     /locus_tag="ACHOUK_000969"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFP9896466.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGTAYGFGADATSEQSVLALSRGVDEIFVRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            23524..23883
                     /gene="gutM"
                     /locus_tag="ACHOUK_000970"
     CDS             23524..23883
                     /gene="gutM"
                     /locus_tag="ACHOUK_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFP9896467.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIADTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            23950..24723
                     /gene="srlR"
                     /locus_tag="ACHOUK_000971"
                     /gene_synonym="gutR"
     CDS             23950..24723
                     /gene="srlR"
                     /locus_tag="ACHOUK_000971"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFP9896468.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVGFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDADIDPAFRQALEEKGIDVIITGESNE"
     gene            24716..25681
                     /gene="gutQ"
                     /locus_tag="ACHOUK_000972"
     CDS             24716..25681
                     /gene="gutQ"
                     /locus_tag="ACHOUK_000972"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFP9896469.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTVGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(25678..27192)
                     /gene="norR"
                     /locus_tag="ACHOUK_000973"
     CDS             complement(25678..27192)
                     /gene="norR"
                     /locus_tag="ACHOUK_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFP9896470.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRSGDEVILEAQHFAFPEVTLPPPEVAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            27379..28818
                     /gene="norV"
                     /locus_tag="ACHOUK_000974"
     CDS             27379..28818
                     /gene="norV"
                     /locus_tag="ACHOUK_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFP9896471.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            28815..29948
                     /gene="norW"
                     /locus_tag="ACHOUK_000975"
     CDS             28815..29948
                     /gene="norW"
                     /locus_tag="ACHOUK_000975"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFP9896472.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLLLFPQTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKMVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPV"
     gene            complement(30076..32328)
                     /gene="hypF"
                     /locus_tag="ACHOUK_000976"
     CDS             complement(30076..32328)
                     /gene="hypF"
                     /locus_tag="ACHOUK_000976"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFP9896473.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPYLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALEDLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQVVLSQHLGDLSDDGIQTQWREALRLMQNIYNFTPQYVV
                     HDAHPGYVSCQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMLGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(32481..33008)
                     /gene="hydN"
                     /locus_tag="ACHOUK_000977"
     CDS             complement(32481..33008)
                     /gene="hydN"
                     /locus_tag="ACHOUK_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFP9896474.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(33157..34167)
                     /gene="ascG"
                     /locus_tag="ACHOUK_000978"
     CDS             complement(33157..34167)
                     /gene="ascG"
                     /locus_tag="ACHOUK_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFP9896475.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHKRGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            34427..35884
                     /gene="ascF"
                     /locus_tag="ACHOUK_000979"
     CDS             34427..35884
                     /gene="ascF"
                     /locus_tag="ACHOUK_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFP9896476.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDALIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPALPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTLTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSAQPTVAKEVSLN"
     gene            35893..37317
                     /gene="ascB"
                     /locus_tag="ACHOUK_000980"
     CDS             35893..37317
                     /gene="ascB"
                     /locus_tag="ACHOUK_000980"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFP9896477.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATGFYHRYKEDIALMAEMGFKVFRTSIAWSRPFPQGD
                     ELTPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMQLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNTNNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVFVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(37441..37911)
                     /gene="hycI"
                     /locus_tag="ACHOUK_000981"
     CDS             complement(37441..37911)
                     /gene="hycI"
                     /locus_tag="ACHOUK_000981"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFP9896478.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(37904..38314)
                     /gene="hycH"
                     /locus_tag="ACHOUK_000982"
     CDS             complement(37904..38314)
                     /gene="hycH"
                     /locus_tag="ACHOUK_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFP9896479.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(38311..39078)
                     /gene="hycG"
                     /locus_tag="ACHOUK_000983"
     CDS             complement(38311..39078)
                     /gene="hycG"
                     /locus_tag="ACHOUK_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFP9896480.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(39078..39620)
                     /gene="hycF"
                     /locus_tag="ACHOUK_000984"
     CDS             complement(39078..39620)
                     /gene="hycF"
                     /locus_tag="ACHOUK_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFP9896481.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(39630..41339)
                     /gene="hycE"
                     /locus_tag="ACHOUK_000985"
     CDS             complement(39630..41339)
                     /gene="hycE"
                     /locus_tag="ACHOUK_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFP9896482.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(41357..42280)
                     /gene="hycD"
                     /locus_tag="ACHOUK_000986"
     CDS             complement(41357..42280)
                     /gene="hycD"
                     /locus_tag="ACHOUK_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFP9896483.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVASSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(42283..44109)
                     /gene="hycC"
                     /locus_tag="ACHOUK_000987"
     CDS             complement(42283..44109)
                     /gene="hycC"
                     /locus_tag="ACHOUK_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFP9896484.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLSVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(44106..44717)
                     /gene="hycB"
                     /locus_tag="ACHOUK_000988"
     CDS             complement(44106..44717)
                     /gene="hycB"
                     /locus_tag="ACHOUK_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFP9896485.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(44842..45303)
                     /gene="hycA"
                     /locus_tag="ACHOUK_000989"
     CDS             complement(44842..45303)
                     /gene="hycA"
                     /locus_tag="ACHOUK_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFP9896486.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            45515..45865
                     /gene="hypA"
                     /locus_tag="ACHOUK_000990"
     CDS             45515..45865
                     /gene="hypA"
                     /locus_tag="ACHOUK_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFP9896487.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            45869..46741
                     /gene="hypB"
                     /locus_tag="ACHOUK_000991"
     CDS             45869..46741
                     /gene="hypB"
                     /locus_tag="ACHOUK_000991"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFP9896488.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAC
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            46732..47004
                     /gene="hypC"
                     /locus_tag="ACHOUK_000992"
     CDS             46732..47004
                     /gene="hypC"
                     /locus_tag="ACHOUK_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFP9896489.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            47004..48125
                     /gene="hypD"
                     /locus_tag="ACHOUK_000993"
     CDS             47004..48125
                     /gene="hypD"
                     /locus_tag="ACHOUK_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFP9896490.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     QRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            48122..49132
                     /gene="hypE"
                     /locus_tag="ACHOUK_000994"
     CDS             48122..49132
                     /gene="hypE"
                     /locus_tag="ACHOUK_000994"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFP9896491.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPTNIHWG
                     AQTLTAGDILLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            49206..51284
                     /gene="flhA"
                     /locus_tag="ACHOUK_000995"
     CDS             49206..51284
                     /gene="flhA"
                     /locus_tag="ACHOUK_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFP9896492.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(51321..51674)
                     /gene="ygbA"
                     /locus_tag="ACHOUK_000996"
     CDS             complement(51321..51674)
                     /gene="ygbA"
                     /locus_tag="ACHOUK_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFP9896493.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKCP
                     VPELPEKYRPKKPRE"
     gene            complement(51751..51885)
                     /locus_tag="ACHOUK_000997"
     CDS             complement(51751..51885)
                     /locus_tag="ACHOUK_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9896494.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSVG
                     "
     gene            51961..54522
                     /gene="mutS"
                     /locus_tag="ACHOUK_000998"
     CDS             51961..54522
                     /gene="mutS"
                     /locus_tag="ACHOUK_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFP9896495.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTRLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            54628..55284
                     /gene="pphB"
                     /locus_tag="ACHOUK_000999"
     CDS             54628..55284
                     /gene="pphB"
                     /locus_tag="ACHOUK_000999"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFP9896496.1"
                     /translation="MPSTRYQKINTHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLNVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQKEATDLLLKFHHLPHIIEITNDNIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIK
                     "
     gene            complement(55325..55561)
                     /locus_tag="ACHOUK_001000"
     CDS             complement(55325..55561)
                     /locus_tag="ACHOUK_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HFP9896497.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLAEGKR"
     gene            complement(55572..56999)
                     /locus_tag="ACHOUK_001001"
     CDS             complement(55572..56999)
                     /locus_tag="ACHOUK_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HFP9896498.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNTPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTRPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            complement(56999..57592)
                     /locus_tag="ACHOUK_001002"
     CDS             complement(56999..57592)
                     /locus_tag="ACHOUK_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFP9896499.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAATISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPYARRWQGLPQARNFSQENE"
     gene            57739..58146
                     /locus_tag="ACHOUK_001003"
     CDS             57739..58146
                     /locus_tag="ACHOUK_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP9896500.1"
                     /translation="MTLRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDAADKRRRFVWLTAEGEKILAAA
                     IPIGDSVDEEFLGRLSAEEQELFVQLVRKMMNT"
     gene            complement(58266..59258)
                     /gene="rpoS"
                     /locus_tag="ACHOUK_001004"
     CDS             complement(58266..59258)
                     /gene="rpoS"
                     /locus_tag="ACHOUK_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFP9896501.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(59321..60415)
                     /gene="nlpD"
                     /locus_tag="ACHOUK_001005"
     CDS             complement(59321..60415)
                     /gene="nlpD"
                     /locus_tag="ACHOUK_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFP9896502.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPAHTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(60600..61226)
                     /gene="pcm"
                     /locus_tag="ACHOUK_001006"
     CDS             complement(60600..61226)
                     /gene="pcm"
                     /locus_tag="ACHOUK_001006"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFP9896503.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(61220..61981)
                     /gene="surE"
                     /locus_tag="ACHOUK_001007"
     CDS             complement(61220..61981)
                     /gene="surE"
                     /locus_tag="ACHOUK_001007"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFP9896504.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(61962..63011)
                     /gene="truD"
                     /locus_tag="ACHOUK_001008"
     CDS             complement(61962..63011)
                     /gene="truD"
                     /locus_tag="ACHOUK_001008"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFP9896505.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELVELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(63008..63487)
                     /gene="ispF"
                     /locus_tag="ACHOUK_001009"
     CDS             complement(63008..63487)
                     /gene="ispF"
                     /locus_tag="ACHOUK_001009"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFP9896506.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(63487..64197)
                     /gene="ispD"
                     /locus_tag="ACHOUK_001010"
     CDS             complement(63487..64197)
                     /gene="ispD"
                     /locus_tag="ACHOUK_001010"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFP9896507.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVYA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGAAITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(64216..64527)
                     /gene="ftsB"
                     /locus_tag="ACHOUK_001011"
     CDS             complement(64216..64527)
                     /gene="ftsB"
                     /locus_tag="ACHOUK_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFP9896508.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(64721..65044)
                     /gene="ygbE"
                     /locus_tag="ACHOUK_001012"
     CDS             complement(64721..65044)
                     /gene="ygbE"
                     /locus_tag="ACHOUK_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFP9896509.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLMTVGIMFGALF
                     MWLLG"
     gene            complement(65094..65699)
                     /gene="cysC"
                     /locus_tag="ACHOUK_001013"
     CDS             complement(65094..65699)
                     /gene="cysC"
                     /locus_tag="ACHOUK_001013"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFP9896510.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(65699..67126)
                     /gene="cysN"
                     /locus_tag="ACHOUK_001014"
     CDS             complement(65699..67126)
                     /gene="cysN"
                     /locus_tag="ACHOUK_001014"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFP9896511.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRSRVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGEK"
     gene            complement(67128..68036)
                     /gene="cysD"
                     /locus_tag="ACHOUK_001015"
     CDS             complement(67128..68036)
                     /gene="cysD"
                     /locus_tag="ACHOUK_001015"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFP9896512.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHQNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            68288..69325
                     /gene="iap"
                     /locus_tag="ACHOUK_001016"
     CDS             68288..69325
                     /gene="iap"
                     /locus_tag="ACHOUK_001016"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFP9896513.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPQSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   69407..69619
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=69468..69497
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacaca"
     gene            complement(69715..70008)
                     /gene="cas2e"
                     /locus_tag="ACHOUK_001017"
     CDS             complement(69715..70008)
                     /gene="cas2e"
                     /locus_tag="ACHOUK_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFP9896514.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVAFLPVDNQ"
     gene            complement(70005..70928)
                     /gene="cas1e"
                     /locus_tag="ACHOUK_001018"
     CDS             complement(70005..70928)
                     /gene="cas1e"
                     /locus_tag="ACHOUK_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HFP9896515.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVDQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEKVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRGG"
     gene            complement(70925..71575)
                     /gene="cas6e"
                     /locus_tag="ACHOUK_001019"
     CDS             complement(70925..71575)
                     /gene="cas6e"
                     /locus_tag="ACHOUK_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311637.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFP9896516.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESETFTIECRSFVPELRTGQQLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQAEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEV"
     gene            complement(71557..72303)
                     /gene="cas5e"
                     /locus_tag="ACHOUK_001020"
     CDS             complement(71557..72303)
                     /gene="cas5e"
                     /locus_tag="ACHOUK_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFP9896517.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSAPD
                     LLSAIISRRDYYTDAWWMVAVATTPDAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNASDVLRNAYQQYQDSFRELKVSLPKLQDECWWEGEHDGLVASKILRRRDV
                     PLNRQQWLFGERTINQGPWLSKEEPCTSQE"
     gene            complement(72314..73369)
                     /gene="cas7e"
                     /locus_tag="ACHOUK_001021"
     CDS             complement(72314..73369)
                     /gene="cas7e"
                     /locus_tag="ACHOUK_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFP9896518.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEEKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRMAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTLRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTEQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(73381..73917)
                     /gene="casB"
                     /locus_tag="ACHOUK_001022"
                     /gene_synonym="cse2"
     CDS             complement(73381..73917)
                     /gene="casB"
                     /locus_tag="ACHOUK_001022"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFP9896519.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAALSANVKAIDERLPFAAQLAAVMSKGRFTRLS
                     AVKTPDELLRQLRRVVRLLNGSVNLDSLAEGVFRWCQESDDLLNHHRRHQRPTEFIRI
                     RWALEYYQAGDVDNEQNQ"
     gene            complement(73914..75476)
                     /gene="casA"
                     /locus_tag="ACHOUK_001023"
                     /gene_synonym="cse1"
     CDS             complement(73914..75476)
                     /gene="casA"
                     /locus_tag="ACHOUK_001023"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFP9896520.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQSSFAPKDYRRWDDIWEDGLEAEKLREALLSLEHPFQFGPDSPSFM
                     QDFEVLMGDKVQVASLLPEIPGAQTTKFNKDHFIKRGVTEHVCSHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQAPLWRKLRLNVMPQDEADLPLPKKFDD
                     LVFPWLGPTRTSELAGAVVTDDQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRVPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLNKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLGKWQKEIWLFARQDFDERVFTNPYEPVDLERVMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            complement(75574..>78203)
                     /gene="cas3"
                     /locus_tag="ACHOUK_001024"
     CDS             complement(75574..>78203)
                     /gene="cas3"
                     /locus_tag="ACHOUK_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HFP9896521.1"
                     /translation="RRGEKEGGDGYHLLCWHSLDVAAMGYLMVKRNCFGLADYFRQLG
                     ISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLAPELKIPEGSRKNYEKISHSTLGYWL
                     WNYYLSECEELLPSSSLSSRKLTRVIEMWMSITTGHHGRPPDRIDELDNFLPEDKAAA
                     RDFLLEIKALFPLIEIPTFWDDDEGVELLKQLSWYISATVVLADWTGSSTRFFPRVAH
                     PMDIKDYWQKTLVQAQNALTVFPPKAETAPFTGINTLFPFIEHPTPLQQKVLDLDISQ
                     PGPQLFILEDVTGAGKTEAALILAHRLMAARKAQGLFFGLPTMATANAMYDRLVKTWL
                     AFYSPESRPSLVLAHSARTLMDRFNESLWSGDLVGSEEPDEQTFSQGCAAWFANSNKK
                     ALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKILLADEIHACDAYMSCILEGLIER
                     QARGGNSVILLSATLSQQQRDKLVAAFARGTEGQQEAPFLEKDDYPWLTHVTKSDVNS
                     HRVATRKDVERSVSVGWLHSEQESIARIESAVSQGKCIAWIRNSVDDAIKVHRQLLAR
                     GVIPASSLSLFHSRFAFSDRQRIEMETLARFGKEDGSQRAGKVLICTQVLEQSVDCDL
                     DEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRDGKDERSPPELLILAPVWDDAPGDE
                     WFGSAMRNSAYVYPDHGRIWLTQRVLREQGAIQMPHAARLLIESVYGEDVVMPEGFAR
                     SEQEQVGKYYCDRAMAKKFVLNFKPGYAANINDYLPEKLSTRLAEESVSLWLATCIAG
                     VVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLLEGEAFRQWCIEQRQDPEMANVILV
                     TDDESCGYSAREGLIGKVD"
BASE COUNT        18392 a        21395 c        20097 g        18319 t
ORIGIN      
        1 agcgttcaaa tatatcgaac aaggactttc aaaagaacaa attctggaaa aaactgggct
       61 atcgcttggc gtaaaagacg ccagtctggc cattgaagaa ggcgagatat ttgtcatcat
      121 gggattatcc ggctcgggta aatccacaat ggtacgcctt ctcaatcgcc tgattgaacc
      181 cacccgcggg caagtactga ttgatggtgt ggatattgcc aaaatatccg acgccgaact
      241 ccgtgaggtg cgcagaaaaa agattgcgat ggtcttccag tcctttgcct taatgccgca
      301 tatgaccgtg ctggacaata ctgcgtttgg tatggaattg gccggaatta atgccgaaga
      361 acgccgggaa aaagcccttg atgcactgcg tcaggtcggg ctggaaaatt atgcccacag
      421 ctacccgaat gaactctctg gcgggatgcg tcaacgtgtg ggattagccc gcgcgttagc
      481 gattaatccg gatattttat taatggatga agccttctcg gcgctcgatc cattaattcg
      541 caccgagatg caggatgagc tggtaaaatt acaggcgaaa catcagcgca ccattgtctt
      601 tatttcccac gatctcgatg aagccatgcg tattggcgac cgaattgcca ttatgcaaaa
      661 tggtgaagtg gtacaggtcg gcacaccgga tgaaattatc aataatccgg cgaatgatta
      721 tgtccgtacc ttcttccgtg gcgttgatat tagtcaggta ttcagtgcga aagatattgc
      781 ccgccggaca ccgaatggct taattcgtaa aacccctggc ttcggcccac gttcggcact
      841 gaaattattg caggatgaag atcgtgaata tggctacgtt atcgaacgcg gtaataagtt
      901 tgtcggcgca gtctccatcg attcgcttaa aaccgcgtta acgcagcagc aaggtcttga
      961 tgcggcgctg attgatgcgc cgttagcagt cgatgcacaa acgcctctta gcgagttgct
     1021 ctctcatgtc ggacaggctc cctgtgcggt gcccgtggtc gacgaggacc aacagtatgt
     1081 cggcatcatt tcgaaaggaa tgctgctgcg cgctttagat cgtgaggggg taaataatgg
     1141 ctgatcaaaa taatccgtgg gataccacgc cagcggcgga cagtgccgcg caatccgcag
     1201 acgcctgggg tacaccggcg actgcaccga ctgacggcgg tggtgctgac tggctgacca
     1261 gtacgcctgc gccaaacgtc gagcatttta atattctcga tccgttccat aaaacgctga
     1321 tcccgctcga cagttgggtc actgaaggga tcgactgggt cgttacccat ttccgtcccg
     1381 tcttccaggg cgtgcgcgtt ccggttgatt atatcctcaa cggtttccag caattgctgc
     1441 tgggtatgcc cgcaccggtg gcgattatcg ttttcgctct catcgcctgg cagatttccg
     1501 gggtcggaat gggcgtggcg acgctggttt cgctgattgc catcggcgca atcggtgcct
     1561 ggtcgcaggc aatggttact ctggcgctgg tgttaaccgc cctgctgttc tgtatcgtca
     1621 tcggtttgcc gttggggatc tggctggcga gaagtccgcg agcggcgaaa attattcgtc
     1681 cactgcttga tgccatgcag accacgccag cgtttgttta tctggtgcca atcgtcatgc
     1741 tatttggtat cggtaacgtg ccgggcgtgg tggtgacgat catctttgct ctgccgccga
     1801 ttatccgtct gaccattctg gggattaacc aggttccggc ggatctgatt gaagcctcgc
     1861 gctcattcgg tgccagcccg cgccagatgc tgttcaaagt tcagttaccg ctggcgatgc
     1921 cgaccattat ggcgggcgtt aaccagacgc tgatgctggc cctttctatg gtggtcatcg
     1981 cctcgatgat tgccgtcggc gggttgggtc agatggtact tcgtggtatc ggtcgtctgg
     2041 atatggggct tgccaccgtt ggcggcgtcg ggattgtgat cctcgccatt atcctcgatc
     2101 gtctgacgca ggccgttggg cgcgactcac gcagtcgcgg caaccgtcgc tggtacacca
     2161 ctggccctgt cggtctgctg acccgcccat tcattaagta actctgcact tgcccggtga
     2221 cgccgggcat atcaccctgc caaaaaaagg aataacaatg cgacatagcg tactttttgc
     2281 gacagcgttt gccacgctta tctctacaca aacttttgct gccgacctgc cgggcaaagg
     2341 cattactgtt aatccggttc agagcaccat caccgaagaa actttccaga cgctgctggt
     2401 cagccgcgcg ctggagaaat taggttatac cgtcaataaa cccagcgaag tggattacaa
     2461 cgttggctac acctcgcttg cttccggcga tgcaaccttc accgccgtga actggacgcc
     2521 actgcatgac aacatgtacg aagctgccgg tggcgataag aaattttatc gtgaaggggt
     2581 atttgttaac ggcgcggcac agggttacct gatcgataag aaaaccgccg accagtacaa
     2641 aatcaccaac atcgcacaac tgaaagatcc gaagatcgcc aaactgttcg ataccaacgg
     2701 cgacggaaaa gcggatttaa ccggttgtaa ccctggctgg ggctgcgaag gtgcgatcaa
     2761 ccaccagctt gccgcgtatg aactgaccaa taccgtgacg cataatcagg ggaactacgc
     2821 ggcgatgatg gccgacacca tcagtcgtta caaagagggc aaaccggtgt tttactacac
     2881 ctggacgccg tactgggtga gtaatgagct gaagccaggc aaagatgtgg tctggttgca
     2941 ggtgccgttc tctgcactgc cgggcgataa aaatgccgat accaaactgc cgaatggcgc
     3001 gaattacggc ttcccggtca gcaccatgca tatcgttgcc aataaagcct gggccgagaa
     3061 aaacccggca gcagcgaaac tgtttgccat tatgcagttg ccagtggcag atattaacgc
     3121 ccagaacgcc attatgcatg acggcaaagc ctcagaaggc gatattcagg gccacgttga
     3181 tggctggatc aaagcccacc agcaacagtt cgatggctgg gtgaatgagg cgttggcagc
     3241 gcagaagtaa tttttattcg ggcggataag gcgtttacgc cgcatccgcc gttctgtaca
     3301 caatgcctga tgcgacgctg gcgcgtctta tcatgcctac aaaccacacc gcacatttca
     3361 acaatctatc tttcatccca tattcatcaa catccgctat tattgatttc cagcttaatc
     3421 atcacctgat gaacaaaaat aatgaccaaa cctaatcatg agcttagccc ggcgctaatc
     3481 gtgctgatgt ctatcgccac cggtctggcg gtcgccagca actattacgc ccagccattg
     3541 ctcgacacca tcgcgcgtaa cttttccctt tccgccagtt cggcaggctt tattgttacc
     3601 gccgcgcagt tgggctatgc cgccggtctg ctgtttcttg ttcccctcgg tgatatgttt
     3661 gaacgccgcc gcctgattgt ctcgatgacc ttactggcag cgggcggtat gttaatcact
     3721 gccagcagtc agtcactggc gatgatgatc ctcggtacgg cattaaccgg tttattctca
     3781 gtcgtggcac aaattctggt tccgctggca gcgacgctgg cttcaccgga taaacgcggc
     3841 aaagtggttg gcaccattat gagcggtctg ctgttgggga tattgctggc acgaaccgtt
     3901 gccgggttac tggcaaacct cggcggctgg cgcaccgtct tttgggttgc ctcggtgtta
     3961 atggctctga tggcgctggc attatggcgt ggtctgccac aaatgaaatc agaaacccac
     4021 ctcaactacc cacagttgtt gggttccgtt ttcagcatgt ttatcagcaa taagatcctg
     4081 cgcacccgcg cgttgctggg ctgcctgacc tttgccaact tcagcattct ctggacctca
     4141 atggcctttt tgcttgccgc tccacctttt aactacagcg atggtgtaat tggtctgttt
     4201 gggcttgcgg gagctgccgg agcgttgggc gctcgtccgg cgggcggttt tgccgacaag
     4261 ggcaaatcgc accacaccac aactttcggt ctgcttctgc tgttactttc atggctggca
     4321 atctggtttg gtcacacttc cgtactggcg ttgattatcg gcattctggt gctggacctc
     4381 accgtacagg gcgtgcatat cactaaccag acggtaattt atcgaataca tcctgatgcg
     4441 cgtaatcgcc tgaccgcagg ttacatgacc agctacttta ttggcggtgc cgccggttcg
     4501 ctaatttcag cctcagcctg gcaacatggc ggttgggctg gcgtttgtct ggctggcgcg
     4561 acgattgccc tggttaactt actggtctgg tggcgaggtt ttcatcgtca ggaagccgca
     4621 aattaagcaa tcagcaaccg ttttcgtggc tttacactta taagggtgtt aagaagccca
     4681 tcagtctgat aaggttaaga tattcattca gtctatttat aatattaaca atcgttaagc
     4741 gtacactcta tggaaagccc tactccacag cctgctcctg gttcggcgac cttcatggaa
     4801 ggatgcaaag acagtttacc gattgttatt agttatatcc cggtggcctt tgcgttcggt
     4861 ctgaatgcga cccgtctggg attctctcct ctcgaaagcg tttttttctc ctgcatcatt
     4921 tatgcaggcg cgagccagtt cgtcattacc gcgatgctgg cagcagggag tagtttgtgg
     4981 gttgctgcac tgaccgtcat ggcaatggat gttcgccatg ttttgtatgg cccgtcactg
     5041 cgtagccgta ttattcagcg tctgcaaaaa tcgaaaaccg ccctatgggc gtttggcctg
     5101 acggatgagg tttttgccgc cgccaccgca aaactggtac gcaataatcg ccgctggagc
     5161 gagaactgga tgatcggcat tgccttcagt tcatggtcat cgtgggtatt tggtacggta
     5221 ataggggcat tctccggcag cggcttgctg caaggttatc ccgccgttga agctgcatta
     5281 ggttttatgc ttccggcact ctttatgagt ttcctgctcg cctcttttca gcgcaaacaa
     5341 tctctttgcg ttaccgcagc gttagttggt gcccttgcag gcgtaacgct attttctatt
     5401 cccgtcgcca ttctggcagg cattgtctgt ggctgcctca ctgcgttaat ccaggcattc
     5461 tggcaaggag cgcccgatga gctatgaggt tctgctgctt gggttactgg ttggcgcggc
     5521 gaattattgc ttccgctatt tgccgctgcg cctgcgtgtg ggtaatgccc gcccaaccaa
     5581 acgtggcgcg gtaggtattt tgctcgacac cattggcatc gcctcgatat gcgctctgct
     5641 ggttgtctct accgcaccag aagtgatgca cgatacacgc cgtttcgtgc ccacgctggt
     5701 cggcttcgcg gtactgggtg ccagtttcta taaaacacgc agcattatca tcccaacact
     5761 gcttagtgcg ctggcctatg ggctcgcctg gaaagtgatg gcgattatat aacccacaag
     5821 aatcattttt ctaaaacaat acatttactt tatttgtcac tgtcgttact atatcggctg
     5881 aaattaatga ggtcataccc aaatggatag ttcgtttacg cccattgaac aaatgctaaa
     5941 atttcgcgcc agccgccacg aagattttcc ttatcaggag atccttctga ctcgtctttg
     6001 catgcatatg caaagcaagc tgctggagaa ccgcaataaa atgctgaagg ctcaggggat
     6061 taacgagacg ttgtttatgg cgttgattac gctggagtct caggaaaacc acagtattca
     6121 gccttctgaa ttaagttgtg ctcttggatc atcccgtacc aacgcgacgc gtattgccga
     6181 tgaactggaa aaacgcggtt ggatcgaacg tcgtgaaagc gataacgatc gccgctgcct
     6241 gcatctgcaa ttaacggaaa aaggtcacga gtttttgcgc gaggttttac caccgcagca
     6301 taactgcctg catcaactct ggtccgcgct cagcacaaca gaaaaagacc agctcgagca
     6361 aatcacccgc aaattgctct cccgtctcga ccagatggaa caagacggtg tggttctcga
     6421 agcgatgagc taacgcgtca tctcgctcaa aaatccagat ttataaaaga aaaaatgacc
     6481 ggccagcatc gcaacatgct ggcctttttg gcaagcaggt cggctcagcc gatgagttaa
     6541 gaagatcgtg gagaacaata tgagcgcaaa tgcggagact caaaccccgc agcaaccggt
     6601 aaagaagagc ggcaaacgta agcgtctgct cctccttctc accttgctct ttataattat
     6661 tgccgtagcg atagggattt attggttttt ggtactgcgt cacttcgaag aaaccgatga
     6721 cgcatacgtg gcagggaatc aaattcaaat tatgtctcag gtgtctggca gcgtgacgaa
     6781 agtctgggcc gataacaccg attttgtaaa agaaggcgac gtgctggtca ctctcgaccc
     6841 gacagatgct cgccaggcgt ttgaaaaagc caaaactgca ctggcttcca gcgttcgcca
     6901 aacccaccag cagatgatta acagcaagca gttgcaggcg aatattgagg tgcaaaaaat
     6961 cgccctcgcg aaagcacaaa gcgactacaa ccgccgtgtg ccgctgggca atgccaacct
     7021 gattggtcgc gaagagctgc aacacgcccg cgacgccgtt accagtgccc aggcgcaact
     7081 ggacgtcgcg attcaacaat acaatgccaa tcaggcgatg attctgggga ctaaactgga
     7141 agatcagcct gccgtgcaac aggctgccac cgaagtacgt aacgcctggc tggcgctgga
     7201 gcgtactcgt attgtcagtc cgatgaccgg ttatgtctcc cgccgcgcgg tacagcctgg
     7261 ggcgcaaatt agcccaacga cgccgctgat ggcggtcgtt ccagccacca atatgtgggt
     7321 ggatgccaac tttaaagaga cgcagattgc caatatgcgt atcggtcagc cggtcactat
     7381 caccaccgat atttacggcg atgatgtgaa atacaccggt aaagtggttg gtctggatat
     7441 gggcacaggt agcgcgttct cactgcttcc ggcgcaaaat gcgaccggta actggatcaa
     7501 agtcgttcag cgtctgcctg tgcgtatcga actggaccag aaacagctgg agcaataccc
     7561 gctgcgtatc ggtttatcca cgctggtgag cgtcaatacc actaaccgtg acggtcaggt
     7621 actggcaaat aaagtacgtt ccactccggt agcggtaagc accgcgcgtg aaatcagcct
     7681 ggcacctgtc aataaactga tcgacgatat cgtaaaagct aacgctggct aatccagagg
     7741 tgcgtgtgat gcaacagcaa aaaccgctgg aaggcgcgca actggtcatt atgacgattg
     7801 cgctgtcact agcgacattc atgcaggtgc tggactccac cattgctaac gtggcgatcc
     7861 ccactatcgc cgggaatctt ggctcatcgc tcagccaggg aacgtgggta atcacttctt
     7921 tcggggtggc gaatgccatc tcgatcccgc ttaccggctg gctggcaaag cgcgtcgggg
     7981 aagtgaaact gttcctctgg tcaaccatcg cctttgctat tgcgtcgtgg gcgtgtggtg
     8041 tctccagcag cctgaatatg ctgatcttct tccgcgtgat tcaggggatt gtcgccgggc
     8101 cgttgatccc gctttcgcaa agtctattgc tgaataatta cccgccagcc aaacgctcaa
     8161 tcgcgctggc gttgtggtcg atgacggtga ttgtcgcgcc aatttgcggc ccaatcctcg
     8221 gcggatatat cagcgataat taccactggg gctggatatt cttcatcaac gtgccgattg
     8281 gcgtggcggt ggtgttgatg acactgcaaa ctctgcgcgg acgtgaaacc cgcaccgaac
     8341 ggcggcggat tgatgccgtg gggctggcac tgctggttat tggtatcggc agcctgcaga
     8401 ttatgctcga ccgcggtaaa gagctggact ggttttcatc acaggaaatt atcatcctta
     8461 ccgtggtggc ggtggtggct atctgcttcc tgatcgtctg ggagctgacc gacgataacc
     8521 cgatagtcga tctgtcgttg tttaagtcgc gcaacttcac catcggctgt ttgtgtatca
     8581 gcctcgcgta tatgctctac ttcggcgcga ttgttctgct gccgcagttg ttgcaggagg
     8641 tctacggtta cacggcgacc tgggcaggtt tggcctctgc gccggtaggg attattccag
     8701 tgatcctgtc gccgattatc ggccgcttcg cgcataaact ggatatgcgc cgactggtaa
     8761 ccttcagctt tattatgtat gccgtctgct tctactggcg tgcctatacc tttgaaccag
     8821 gtatggattt tggcgcgtcg gcctggccgc agtttatcca ggggtttgcg gtggcctgct
     8881 tctttatgcc gctgaccacc attacgctgt ctggtttgcc accggaacga ctggcggcgg
     8941 catcgagcct ctccaacttt acgcgtacgc tggcggggtc tatcggcacg tcgataacca
     9001 caaccatgtg gaccaaccgc gagtcgatgc accatgcgca gttgactgag tcggtaaacc
     9061 cgttcaaccc gaatgcccag gcgatgtaca gtcaactgga agggcttggg atgacacaac
     9121 agcaggcatc aggctggatt gcccagcaga tcaccaatca ggggctgatt atttccgcca
     9181 atgagatctt ctggatgtcg gccgggatat tcctcgtctt gctggggctg gtgtggtttg
     9241 ctaaaccgcc ctttggcgca ggtggcggcg gaggcggtgc gcactaagta caactaagcc
     9301 agttcatttg aactggcttt tttcaattaa ttgtgaagat agtttactga ctaaatgtgc
     9361 agttcctgca acttctcttt cggcagcgcc agttcttcgt tgctgttgat gcgcacgtca
     9421 cgttccagaa tgctacgcgc aatatcctgc gcttcctgca acgagtgcat ctggtaagtg
     9481 ccacactggt agacgttcag ctccggaatc tgattctgat cctgcacttt cagcacgtct
     9541 tccattgccg ctttccagac atcagcaaca cgctgctcat ctggcgtacc aatcagactc
     9601 atataaaaac cggtgcggca gcccattggc gagatatcga taatctctac accattaccg
     9661 ttaagatggt tacgcataaa accagcaaac aggtgctcca gggtatggat ccctctttct
     9721 ggcatcactt ctttgttcgg cacgcagaag cgcagatcga acacggtgat tgcgtcgcca
     9781 tgcggggtgt tcattgtttt cgccacccga actgcaggcg cttccatccg ggtatgatcg
     9841 actgtgaagc tatctaacaa cggcatttag ccacctccgg taattttttt aaaaattttc
     9901 tgaactcttt cttcccaggc gagtctgagt atatgaaaga cgcgcatttg ttatcatcat
     9961 ccctgaattc agagatgaaa ttttggccac tcatgagtgg cctttttctt ttctgtcagg
    10021 cgtgtttttc cagccacacc gcaaacggtt cggtatctgc ggcttccatt tcctgctgac
    10081 ggcgttcaga tgcctcgcgc tcggctacaa aatcctcttc gcgcagaatt tccagcggct
    10141 cttcacgcag cagattacgg tacgcttcag caaacgcttt gcctgttccg ccaataccag
    10201 tatcaatcat agaccttaag atacgggcag agaaagtcag atcgggatta tcgaagcagg
    10261 caaccagttc atcacacact ttctgatacg cttcgccgcc gttaatgcta tccagcgttt
    10321 gcgcgacgcg tttcagatcg cggaacagat ctttacccac ctgcggtaac gggaactgtg
    10381 cggtttcgca gccgataccc agcgtcagac ccggtttgcg accttcgagg atcacccggt
    10441 tccagttaac gcgtgtacag gcaagttcgc tactgctcat ttccggtgca tcagccagcg
    10501 cacaccagac cataaacagg tcgaggaatc gcacctgctg ttcatctaca ccaatcggcg
    10561 agaacgggtt gatgtccagc gaacgcactt caatatattc aatgccgcca cgtaacagcg
    10621 catcagaagg cgactcgccg ctgcgggtaa cgcgttttgg acgaatcggc gcgtacagtt
    10681 cgttttcaat ctgcagcacg ttgctgttga tttgcagcct cttaccgtct ttatcaatac
    10741 caatcttcgc gtactcttcc gatggcgttt tgattgcctg tttcaggccc gctacgtact
    10801 cgtaaagatc gttgaaggta ataccaagat tgctttgcga tttattggta tagccgagat
    10861 cgctcaaacg aagagaggtc gcatacggca ggtaatacat accgcactcg gttttctcaa
    10921 acggcagcga cgttggttta ccttgcagga atgaagagca tattgccgga gacgcgccaa
    10981 acagataagg aatgacccaa ccgaagcgat agtagttgcg aataacgcgg aaatagcccg
    11041 cagagatttt ctctttagca tcagcgcccg aaatatcgcc acacttcgct tgccagaatg
    11101 ccataggcaa agagaagttg taatgcacgc cggagatggt ttgcatcagc gcgccgtagc
    11161 gatttttcag cccttcacgg tacagcgttt taaagcgtcc ggtgttagaa gtgccgtact
    11221 gcgccagttc gatgtcctga ccttctgcga tgtagcatgg catacttaac ggccacatcc
    11281 gctcatcgcc catattgcgc gccgtataac gatgcagatc gcgcataaag gtcagcatat
    11341 gttcaatatc accatccact ggtgtaatga attccagcaa tgcttccgca aaatcggtag
    11401 taatccattt atgcgtcagt gcggaaccta atgcttcagg atgacctgtt gttgccagtg
    11461 tgccatcagc attaacacgc aaagtttcgc gctccagccc acgctgtatc ccctttaacg
    11521 cctgaggatg tttttccagc caggccagcg cctgtgatac gtccgggatc aaattgacct
    11581 cccgcctgtc aaaatcgttt taattagcat aactgtaatg gtgaccatat gtgcaggcct
    11641 acaattagtg ccaccacatc atgccctgaa cggtcgctgc tgcaactgca acatagcgta
    11701 acgctttacc aaggcacaaa aaaaagatta ccggccccca ggagatgcgc atccatcccg
    11761 ctaacagaca cagtaaatcg ccaaccacgg gcatccagct taataatagc gtgactgcac
    11821 catagcgttt cagccagccg gtagctttct cttgccagcg cgatgtttta cgcaatggac
    11881 agaaacgccc aaggataacg ttagttaacc ctccaaggct attacccatt gttgctgtta
    11941 aaactaaaac ccagggatga ctgatcccag aaagcaacat tgccaccagc acgacttcgg
    12001 agttgccggg taatagtgta gcgctgagaa aactactggc aaacaacgag aaaagcgata
    12061 acgcttcact cacagcaagc gaacatccac agcgtccatg cctgctgcac gggccgcctg
    12121 aataccgaaa tcggcatctt caaagaccac acactgcgtc ggttgcacgc ccatacgctg
    12181 cgcgcacaac aaaaatgtgt ctggcgcggg tttatggtgt ttgacgtgat cggcagcgac
    12241 gacggcgtca aaataacggc gtaatcccag gtgagccagc aatgcctcag cgatggcgct
    12301 ttcactcccc gttcctacag ccattgggcg acgaccatgc caacttttca ccacttcaac
    12361 aagaggaagc ggctcgacac tatccagcag catacttctt actgcttctg ttttttcacg
    12421 cgctaacgca tgcgggtcga gatcggcctg attcagctca ataattgcct gagcaatacg
    12481 ccaggtgggc gatccattaa gcgcaatcat cgcctgaaca tcgtactgaa gaccgtagtg
    12541 ccctaatact tcgcgccacg ctttacggtg cgtaggctcc gtatccagga ttgtgccatc
    12601 catatcaaaa attaaacctg cataacgctc gtacatggtc ttctcgcaag tcgaaaaata
    12661 tgacgctact ttagcgtaat tgcttgattt tgtcgctgat ggcgaaggag aagaaaattc
    12721 aggaaatgga taaagtagtt atttagaaat gagatatttt tgaaggaaat tttttggaga
    12781 agatggtgca tccgggagga tgactcactg gcgttcgccc ttcgggccgt tgctaaagca
    12841 acgttatcct tcctggtgcc tgcggttaat tccgctacct cttcgatatc ttcattgctg
    12901 aagatttcgg agaatatggt gcatccggga ggattcgaac ctccgaccgc tcggttcgta
    12961 gccgagtact ctatccagct gagctacgga tgcatcggga aaatttattt tactgctgat
    13021 attgatatcg ctactaaagc catatcaaat aagaaaatcc atcttaacca tcatcactga
    13081 tgattacgga gaatatggtg catccgggag gattcgaacc tccgaccgct cggttcgtag
    13141 ccgagtactc tatccagctg agctacggat gcatcgggaa atttatttta ctgctgatat
    13201 tgataccgct actaaagctg tatcaagtaa gagatggtgc atccgggagg attcgaacct
    13261 ccgaccgctc ggttcgtagc cgagtactct atccagctga gctacggatg catcgggaaa
    13321 cttactttac tgcagatttt ttgataccgc tactaaagcc gtatcaagta agagatggtg
    13381 catccgggag gattcgaacc tccgaccgct cggttcgtag ccgagtactc tatccagctg
    13441 agctacggat gcaaatggcg gtgaggcggg gattcgaacc ccggatgcag cttttgaccg
    13501 catactccct tagcagggga gcgccttcag cctctcggcc acctcaccac acgcctctta
    13561 cgagtgcttc gaagaacttg tttatcgctc atcgtcgctg cgtggcgcac atattacttt
    13621 ctgagactta taagtcaaac aatttttccc acacttttat cgtttgcaca cttcacgctc
    13681 aattagtctg taaaaacgtc aaaaagagtg ttttatctac agaaaaacgg aggtctggca
    13741 gatagcagta atgcaaaaaa atggagactt aagttgaatg aacgggagta aagcgaaaag
    13801 actatagagt gaaggagaaa ttttgagggt gcgtctcacc gataaagatg agacgcggaa
    13861 agattagtaa ctggactgct gggatttttc agcctggata cgctggtaga tctcttcacg
    13921 gtgaacagaa acttccttcg gggcatttac gccaatacgt acctggttgc cctttacccc
    13981 taaaactgtc acggtgacct catccccaat catgagggtc tcaccaactc gacgagtcag
    14041 aatcagcatt ctttgctcct tgaaagatta aaagagtcgg gtctctctgt atcccggcat
    14101 tatccatcat ataacgccaa aaagtaagca atgacaaaca cattacatgt aagcagtcat
    14161 ggcattacat tctgttaaac ctaagtttag ccgatataca caacttcaac ctgactttat
    14221 cgttgtcgat agcgttaatg cgaatgccgt gaagcgagtc cacggcattg cctgacgctt
    14281 atattattgc aatttcgcgc tgacccagcc tttcacactg gctaacgctg caggtaaggc
    14341 cgcagcatcc gtaccaccgg cttgcgccat gtcaggacgt ccaccaccct tgccgcccac
    14401 ctgctgagcg accataccaa tcagttcccc tgctttcaca cgatctgtga cgtccttaga
    14461 tacgcctgca atcagagaaa ccttaccttc ggctaccgtt gccagcacga taattgtcga
    14521 ccccagctga ttttttaaat cgtcaaccat ggtacgcaac attttcggct caacaccgct
    14581 aagctcgcta accaacagct taacaccatt aacatcaatt gccttactgg aaagatttgc
    14641 gctctcctgt gcggcagctt gttctttaag ctgttgtagt tctttttcta actgacgcgt
    14701 acgttccagt actgagcgca ctttatccgc cagattattg ctatcgcctt tcagcagatg
    14761 cgcgacttcg cttaagcgat cgctgtctgc atgaacggtg gtgatagcgc cttctccggt
    14821 taccgcttcg atacgacgaa cgcctgcagc agtacccgat tcagagatga tgcggaacag
    14881 accaatatca ccagtgcggc tggcgtgagt accgccacac agctcggtgg agaaatcgcc
    14941 catgctcagc acgcgtacac gctcatcata cttctcgccg aacagcgcca tcgcaccttt
    15001 cgctttcgcc gcttcgagat ccatgatgtt ggtttcgatc ggcaaattgc gacgaatctg
    15061 tgcgttcacc aggtcttcga ccgcacgaat ctcttccggt ttcatcgctt cgttgtgtga
    15121 gaagtcgaag cgcagcacct tgtcgttaac cagtgaacct ttctgcgata catgagtccc
    15181 cagaacctgg cgcagcgcag cgtgcatcag gtgcgttgcg gagtgattca aacgaatacg
    15241 ggcgcgacga gcctcatcaa catcagcctg cacagcgtcg cccactttca gagaacccgc
    15301 agcaagttta ccgatgtgac caatcgcctg gccgtatttc tgcgtatctt ccaccacgaa
    15361 ggagaagtta gcgcctttca gttcgccttt atcaccaacc tgaccgccgg attccgcata
    15421 gaatggcgtt tgatccagca cgaccacagc ttcctggcct gcattgatgg catcaaccgc
    15481 tttaccatca acaaacagcg cggtcacttt gccgttcagt tccagatggt catagccttt
    15541 aaattcagat gcactgtcaa cacggatcat tgcgttgtaa tcggcaccaa agccgctggc
    15601 ttcgcgcgcc cgacgacgct gctcttccat tgctgcttca aaaccagctt cgtcaacttt
    15661 gatgttgcgc tcacgacaaa catcagccgt caggtcaacc gggaagccat aggtgtcgta
    15721 cagacggaaa gcagtttcac catccagcgt atcaccagaa agttttgcca gctcttcatc
    15781 cagcaacgcc agaccgcgct ccagagtacg agcaaactgc tcttcttcag tcttcagcac
    15841 ctgctcaacc tgcgcctgct ggcgtttcag gtcttcaccc gcagagccca taacgtcgat
    15901 cagcggacca accagtttgt agaagaaggt ttctttcgcg ccgagcatat taccgtgacg
    15961 cactgcgcga cgaatgatac gacgcagtac ataaccacgg ttttcattgg acggcattac
    16021 gccatccgcg atcaggaacg cacaagaacg aatgtggtca gcgattacgc gcagcgattt
    16081 attgctcaga tcggttgcgc cagtgacttt cgctaccgcc tggatcaacg tgcggaacag
    16141 gtcgatgtca tagttagagt taacgtgttg cagcaccgca gcaatacgct ccagacccat
    16201 accggtatct acagacggct tcggcagcgg ttccatcgtg ccatcggcct ggcggttgaa
    16261 ctgcatgaag acgatgttcc agatctcaat gtagcggtcg ccgtcttctt ccggacttcc
    16321 cggagggcca ccccaaatgt ggtcgccgtg atcgtagaag atttcggtgc acgggccgca
    16381 cggaccagtg tcacccatct gccagaagtt gtcagatgcg tatggcgcac ctttgttatc
    16441 gccgatgcga ataatacgtt cgcgcgggat ccctacttct ttttcccaga tttcgtaggc
    16501 ttcgtcgtcg ctttcataga cggtaaccca cagacgctct ttcggcaggg caaaccattt
    16561 ttcgctggtc agcagttccc atgcaaactg aatggcatcg tgtttgaaat agtcgccgaa
    16621 gctgaagttg cccagcattt cgaagaaggt atggtgacgc gcggtgtaac cgacgttttc
    16681 caggtcgttg tgtttaccac ccgcacgcac gcagcgttgg gaagtggtag cgcgggaata
    16741 attacgcttg tcgagcccaa ggaacacatc cttgaactgg ttcatcccgg cgttggtaaa
    16801 caacaaagtt gggtcgttat ggggtaccag ggagctgctg gcaactacct gatgtccctt
    16861 actatggaaa aagtcgagaa acgcctgacg gatctcagcg gtgctcttgc tcataattat
    16921 cctgaaatca agctaacgaa atatcgccac cagctccagc gtgtcttaac cgccgggctg
    16981 gtaactgaaa agtgggaata agataagttt tcttgactgg gaagtaaaat accgtatgcg
    17041 ttcagtcggc aaaatttcgc caaatatcct ggatatcttc catcagatag ccacgataga
    17101 gcagaaaacg ctggatctta actttttctg aaaagacagt tggcaaaggt tcaccatatt
    17161 ttcgcgtcgc ctgatcgcgc gccagtgcgc accagtcgat gtcacattca cgcatcgctt
    17221 tttctgtcgc ttcgcgggaa atacctttct gattcagttc ctggcgaata cgcgcaggtc
    17281 cataaccttt gcggctacgg ctggcgataa agcgcgcaac aaatcggctg tcatcgagat
    17341 agccatgttc atggcaccag gcaataacgc gctcgtaatc ttctgccgta gcatcaatct
    17401 cttctgggcc atttttgccc ataatcggtg ccgcgagttt acgtcgcagt tcttgctcac
    17461 tgtgatcgcg caccgccaga atgcgtaccg cacgatccaa caggcgagca tatgccgggc
    17521 gacgggacgt tgattctgtc atggcatatc cttacaactt aaaaagcaaa agggccgcag
    17581 atgcgaccct tgtgtatcaa acaagacgat taaaaatctt cgttagtttc tgctacgcct
    17641 tcgctatcat ctacagagaa atccggcgtt gagttcgggt tgctcagcag caactcacgt
    17701 actttcttct cgatctcttt cgcggtttcc gggttatctt tcagccaggc agttgcattc
    17761 gctttaccct gaccgatctt ctcacctttg tagctgtacc acgcgcctgc tttctcgatc
    17821 agcttctctt ttacgcccag gtcaaccagt tcgccgtaga agttgatacc ttcgccgtag
    17881 aggatctgga attcagcctg tttaaacggc gcagcgattt tgttcttcac cactttcacg
    17941 cgggtttcgc tacccaccac gttttcgccc tctttcaccg cgccgatacg acggatgtcg
    18001 agacgaacag aggcgtagaa tttcagcgcg ttaccaccgg tagtggtttc cgggttaccg
    18061 aacatcacac caattttcat acggatctgg ttgatgaaga tcagcagcgt gttggactgc
    18121 ttcaggttac ccgccagctt acgcatcgcc tggctcatca tacgtgccgc aaggcccatg
    18181 tgagagtcgc cgatttcgcc ttcgatttcc gctttcggcg tcagtgccgc cacggagtca
    18241 acgacgataa cgtctactgc gccagaacgc gccagggcgt cacagatttc cagtgcctgc
    18301 tcgccggtgt ccggctggga gcacagcagg ttgtcgatat cgacgcccag tttacgtgcg
    18361 tagattgggt ccagcgcgtg ttcagcatcg ataaacgcac aggttttacc ttcacgctgc
    18421 gctgcggcaa tcacctgcaa cgtcagcgtg gttttaccgg aagattccgg tccgtagatt
    18481 tcgacgatac ggcccatcgg cagaccacct gccccaagcg cgatatccag tgaaagcgaa
    18541 ccggtagaga tggtttccac atccatggaa cggtcttcac ccaggcgcat gatggagcct
    18601 ttaccaaatt gtttctcaat ctggcccagt gctgccgcca acgctttctg tttgttttcg
    18661 tcgatagcca tttttactcc tgtcatgccg ggtaataccg gatagtcaat atgttctgtt
    18721 gaagcaatta tactgtatgc tcatacagta tcaagtgttt tgtagaaatt gttgccacaa
    18781 ggtctgcaat gcatacgcag tagcctgacg acgcaccgca tcacggtcgc cgctgaagca
    18841 ttcccgcagg gtaatgcctt caccgcgggc agtggcaaaa gcaaaccaga cggtgccgac
    18901 aggcttctct tcactgccgc catccggccc ggcgatacca ctaatagaca cggcataatc
    18961 agcacgagcc gctttcagtg cacctatcgc catttccacc acgacgggtt cactcaccgc
    19021 gccatgctgc gccagcgtct cttcgcgtac gccgatcatt tgcgctttgg cttcgttact
    19081 gtaggtgaca aatccgcgtt caaaccaggc ggaactgccg gcaatatcgg taatcacttt
    19141 cgctacccaa ccgccggtac aagactcagc agttgttacg gttgcgccac gggctttcag
    19201 cgcctgccca acctgttcgc ttaactgcat cagttcactg tcagtcatca caacctctgt
    19261 cagtcaaaaa tttatgccgg acaagatagc actttcgtca ggaagatggt gttggcgttg
    19321 caattttacg aggaggattc agaaaaaagc tgattagcca gagggaagct cacgcccccc
    19381 tcttgcaaat agttactgca cgcgcgccag cgccacggct tgtcctaact gccagaccgc
    19441 cattgcgtaa tgggtactgt ggttataacg ggtaatggtg tagaagttcg gcagaccgta
    19501 ccagtactgg taacctgttc caacatccag acgcagcagg ctggcttgtt gatggttgcc
    19561 cagcggctgc tgtggcgtta aacctgccgc ggcaagctgc gaaatgctgt acctggtttt
    19621 gaagccattt ggcaagcctg gagcctgacc gtttgccatt accgcgacct gatcgccttt
    19681 cacccagccg tgcgctttga aataattcgc cacgctaccg atcgcatcaa ccggatccca
    19741 caggttgata tgcccgtcgc cgctgaaatc taccgcatat tgtttgtaag acgacggcat
    19801 aaactgtccg tagcccatcg ccccggcaaa ggaacctttc agattgagcg gatcgtcctg
    19861 ctcgtcgcgc gccatcagca ggaaggtttc cagctcgcca gagaaatact ccgcgcggcg
    19921 tgggtagtta aatgacagcg ttgccagcgc atcgaggatg cgagttttcc ccatcacgcg
    19981 cccccagcgg gtttcaacgc cgataatccc gacgataatt tccggcggta cgccatacac
    20041 ctgccacgcg cgattcaacg catcttcata ctgattccag aaaaccacac cgttctgcac
    20101 attgtccggc gtaataaatt ttttgcgata acgcagccat gcgccgttcg gacctgatgg
    20161 cggtttcacc gatgtggttg gtgcctggtt atccatcagc cgcagtaccg aatccagacg
    20221 tttcgcctgg gagagaattt cctgcaactg ctgacgatcg aaaccgtgtt tgttcaccat
    20281 tctgtcgatg aactgctggg cattcgggtt attagcgaaa tcgccgccca tctgcatcac
    20341 attgtgctgc ggctcaagca ggaagccgcc agacggcgtt ccggtggtcg tttcagtctc
    20401 agtaggtttt ggcttgctgc tacaggcggc aagcaacaca aaaaggggaa gcaatgttac
    20461 ataacgacgc ttgaacatga ggggtccatt taacagattc aaccaggggc aagtatggta
    20521 aagcatcacg ccccgcacaa ggaagcggta gtcactgccc gatacggact ttacataact
    20581 caactcattc tcctcgctat ccttttactc aaactttcaa attaaaatat ttatctttca
    20641 ttttgcgatc aaaataacac tttaaatctt tcaatctgat tagattaggt tgccgtttgg
    20701 taataaaaca ataaatcctg aaggagagaa caatgataga aaccattact catggtgcag
    20761 agtggtttat cgggctgttc caaaagggcg gagaggtgtt taccgggatg gtgaccggca
    20821 ttcttccgct gttgattagc ctgctggtta tcatgaacgc actgattaat tttatcggtc
    20881 agcatcgtat tgaacgtttt gctcaacgtt gcgccggtaa ccctgtttcc cgttacctac
    20941 tgttaccgtg cattggcacg tttgtctttt gcaatccgat gaccttaagc ctgggtcgct
    21001 ttatgccgga aaagtacaaa cccagctact acgcggcggc ctcttatagc tgccactcaa
    21061 tgaatggcct cttcccccat atcaaccctg gcgaactgtt tgttatcttg gcattgccag
    21121 cggtctgaca acgctgaacc tgccacttgg cccactggcg gtgagctatc tgctggttgg
    21181 tctggtcacc aacttcttcc gcggctgggt gaccgatctg accaccgcta tttttgagaa
    21241 aaagatgggc attcaacttg aacaaaaagt tcatctggca ggagcaacat catgacgcat
    21301 attcggatcg aaaaaggaac ggggggctgg ggcggcccgc ttgagctgga aaccacgccg
    21361 ggcaaaaaaa tcgtctatat caccccgccg gtacccggcc tgcgattgtc gacaaactgg
    21421 cacagcttac tggctggcag gctattgacg gatttaaaga aggtgaaccc gcggaggcgg
    21481 aaattggtgt cgcggtaatc gactgtggcg gcacattacg ctgcggcatt tatccgaaac
    21541 ggcgtattcc caccattaat atccactcga cgggcaagtc cggtccactg gcgcagtaca
    21601 ttgtggaaga tatttatgtc tctggcgtaa aagaagaaaa catcactgta gtgggtgatg
    21661 cgacaccaca accctcttcc gtgggccgtg actatgacac cagcaagaaa atcaccgaac
    21721 aaagcgatgg tttactggcg aaggtgggaa tgggtatggg ttctgccgtt gccgtgttgt
    21781 ttcaatctgg tcgtgacacc atcgacactg tattaaaaac cattcttccg tttatggcat
    21841 tcgtctcggc gctcattggc atcattatgg cttctggcct tggtgactgg attgcccacg
    21901 gccttgctcc gctggcgagc catccactgg gtctggtcat gctggcgctc atctgctcct
    21961 tcccgctgct ttcacctttc ctcggcccag gcgcagttat cgcacaggtt atcggcgtat
    22021 tgattggcgt gcagattggt ctcggcaata ttccgccgca tctggcttta cctgcactgt
    22081 ttgccatcaa cgcgcaggcg gcctgcgact tcattccggt cggtttgtcg ctggcggaag
    22141 cccgtcagga cacggttcgc gtcggtgtcc cttctgtact ggtgagccgc tttttaaccg
    22201 gcgcgccgac tgtactgatc gcctggtttg tctccggttt tatctatcaa tagaggctga
    22261 aacatgaccg ttatttatca gaccaccatc acccgtatcg gcgcgagtgc cactgacgcc
    22321 ctcagcgacc agatgctcat cacctttcgt gaaggcgcgc cagcggacct cgaagagtat
    22381 tgcttcattc attgccacgg cgagttgaaa ggtgcactcc atcccggttt gcaatttacg
    22441 ctcgggcagc atcgctatcc ggtgaccgct gttggcagcg tggcggaaga caaccttcgc
    22501 gaactgggtc atgtcaccct gcgcttcgat ggtttaaacg aagcggaatt tccgggcact
    22561 gtccatgtgg caggccctgt acccgacgat atcgcgccgg gatcggtttt gaagtttgaa
    22621 tctgttaagg agtaaaaaat gaatcaggtt gccgttgtca tcggtggtgg gcaaacctta
    22681 ggcgcgttcc tgtgccacgg tctggctgcc gaggggtatc gcgtcgcggt tgtcgatatt
    22741 cagagcgaca aagccgcaaa tgtggcacaa gaaattaacg ccgaatatgg tgaaggaaca
    22801 gcgtatggtt ttggtgctga cgccactagc gagcaaagcg ttctggcgct ctctcgtggg
    22861 gtagatgaaa tctttgttcg tgtggattta ctggtctaca gcgccggaat tgcaaaagca
    22921 gcctttatca gcgacttcca gctcggcgat tttgaccgtt cgctacaggt gaatctggtg
    22981 ggttatttcc tgtgtgcgcg tgaattttcg cgtttgatga tccgcgatgg gattcaggga
    23041 cgcattattc agatcaactc gaaatccggc aaagtgggca gcaaacacaa ttctggttat
    23101 agcgccgcga aatttggtgg cgtagggctg actcaatcac tggcgctgga tctggcggag
    23161 tacggcatta cggtgcattc actgatgctc ggtaacctgc tgaaatcgcc gatgttccag
    23221 tcgctgttgc cacaatatgc caccaagctg ggtatcaaac cggatcaagt cgagcagtat
    23281 tacatcgaca aagtaccgct caaacgcggc tgcgattatc aggatgtgct gaatatgctg
    23341 ctgttctacg ccagtcctaa ggcgtcgtac tgtaccggac agtcgatcaa tgtcaccggc
    23401 ggtcaggtga tgttctgatc aacagcggag atccgttaag gatctccgtg agactaatag
    23461 aatgcctgat gcgctacgct catcaggcat acaggacttc cgccactaca ttaaggaaaa
    23521 gttatggtat ccgcactcat cactgtcgcc gttatcgcct ggtgtgcgca actggcctta
    23581 ggcggctggc aaatttctcg ttttaaccgt gccttcgaca cactatgcca gcaagggcgg
    23641 gttggcgtgg gccgttccag cgggcgcttt aaaccgcggg tcgtggtcgc catcgccctg
    23701 gacgatcagc agcgcatcgc cgacaccttg tttatgaaag gactgaccgt cttcgcccga
    23761 ccgcaaaaaa ttcccgcaat taccggtatg catgcgggtg atttacagcc cgatgtgatc
    23821 tttccccatg atccactatc acagaatgct ctatcattgg cgcttaaact gaaacgtgga
    23881 taatttcgtt gtgaatgtta cttgcttgcg aagttatcat tttgaaacct aaatcaggta
    23941 atcacgccca tgaaacctcg tcagcgtcaa gccgccattc tggagtatct gcaaaagcag
    24001 ggaaaatgct cggttgaaga attggcgcaa tattttgaca ccacaggcac aactattcgc
    24061 aaagatctgg tcattctgga acatgccgga accgtcattc gtacttatgg cggagtggtg
    24121 ttgaataaag aggaatccga tccgcctatc gatcataaaa cactcatcaa cacccacaag
    24181 aaagagctga tagcagaagc tgccgttggt tttattcatg atggcgattc gatcattctt
    24241 gatgctggca gtaccgtttt gcagatggtg cctctgctct cgcgctttaa taacatcacg
    24301 gtgatgacca acagcctgca tatcgtcaat gcgttatctg aactggataa tgaacaaact
    24361 atcctgatgc caggcggaac gtttcgtaaa aaatcggcct catttcacgg gcagctggca
    24421 gagaatgcct tcgagcattt caccttcgac aaattattca tgggtaccga cggcatcgat
    24481 ctcaatgcgg gcgtaaccac cttcaacgag gtctataccg tcagtaaggc aatgtgcaat
    24541 gccgcgcgcg aagtgatttt gatggcggac tcatcaaagt ttggccgtaa aagccccaac
    24601 gtagtttgca gtcttgaaag cgtcgataag ctgattaccg acgcagatat cgatccggcg
    24661 tttcgtcagg cgctggaaga gaaagggatc gatgtgatca taaccggaga gagcaatgag
    24721 tgaagcacta ctgaacgcgg gacgtcagac gttaatgctg gaattgcagg aagcaagccg
    24781 tttaccggaa cgtctgggcg atgattttgt tcgcgccgcc aatatcatcc tgcactgcga
    24841 aggcaaagtg gtggtttcgg gaattggcaa atcgggccac attggtaaga aaatcgccgc
    24901 aacgcttgcc agtaccggca caccggcttt ttttgtccat ccggcagaag cgctgcatgg
    24961 cgatctgggg atgatcgaaa gccgcgatgt gatgctgttt atctcttact ccggtggggc
    25021 gaaggaactg gatctgatta ttccgcgtct ggaagacaaa tctatcgcgc tgctggcgat
    25081 gaccggcaaa ccgacgtcac cgctgggcct ggcggcgaaa gcggtgctgg atatctccgt
    25141 agaacgcgaa gcctgcccga tgcaccttgc gccgacctcc agcaccgtca ataccctgat
    25201 gatgggtgac gcgctggcga tggcggtcat gcaggcgcgc ggatttaatg aagaagattt
    25261 tgcccgctcc caccctgctg gagcactggg cgctcgcttg ctgaataaag tgcatcatct
    25321 gatgcgccgt gacgatgcca tcccacaggt ggcgttaacc gccagcgtga tggatgctat
    25381 gctggaactc agccgcaccg gtctggggct ggtggcggta tgtgacgctc aacaacaggt
    25441 acaaggcgtc tttaccgacg gcgatttacg tcgctggctg gttggcggcg gcgcactcac
    25501 cacgccagtc aatgaagcga tgacggtcgg cggcaccacg ttgcaatcgc aaagtcgcgc
    25561 catcgacgcc aaagagatcc tgatgaagcg caaaatcact gccgcaccgg tggtggatga
    25621 aaacggcaaa ctcaccggcg caataaacct gcaggatttc tatcaggccg ggattattta
    25681 atccttcaat cccagacgtt tcgccagccg atgcaggttg gcgacgtcgg tttccagcat
    25741 ccgcgcgcag gcagcccagt tgtgatgatt ttgtgccagt gcctgacgaa tagtttcacg
    25801 ctggaacgct tctgtcgctt cacgcaggtt ttgcttaaca acgggcaccg ccgccacttc
    25861 tggcggcggc aacgtcacct caggaaaagc aaaatgttgc gcctcaagaa tcacttcatc
    25921 gccgctgcgg gtggctctcg ccagcactac cgcccgatga atagcatgtt ccagttcgcg
    25981 cacgtttccc ggaaaactgt agtgttgcag taaatttcgc gccccggcac ttaataccac
    26041 gcgggagagc ccctgccgca aacgacactg ctcgcagaaa taccccgcca gcagaatgac
    26101 atcatcgccc cgctcacgca gcggcggcac cgaaagtgga aacacactca ggcgatgaaa
    26161 caaatcggcg cggaatcgcc ctgccagcac ctcttcgcgt aagtcgcggt tagtcgccgc
    26221 cagcacgcgc acatcgaccc gcaaactacg gtcatcgcca acgcgctgaa tatcgccata
    26281 ctgcaacacc ctcagcagct tggcctgcaa tgccaacgac aactcgccga tctcatcgag
    26341 aaacagcgtg ccgttatccg ccatttcaaa cttcccactg cggttactga tagcgccagt
    26401 aaacgctcct ttcacatgcc cgaacaactc actttccgcc acactttccg gcagtgcggc
    26461 acagttgaga tagaccagcg gattcaccgc ccgtggcgag gcctcatgaa tcgctttcgc
    26521 caccagctcc ttaccggttc ccgtctcacc gctgatcagg acgttgagat cggacgccgc
    26581 cacaatctca atctcttttt tcagttgcgt catgcctggc gacagaccga tcatctgcgt
    26641 ctgtttcacc gcttcaaatg gagcggcatc gcccggcagc atattctggc tttccagttg
    26701 ttcaatcagc aacgcattgc ttaacgctcc cgccgccagc gcggcaatca ggcgtaactc
    26761 ttcgtcgctg aaaacatcga actgatcggg ctgcatcccg tcgagcgtca atgcgccgat
    26821 caggttttgc ccggcaaaca gcggcagacc aacgcaggcg tgaaccttca gactctcctg
    26881 ccctggaatc aaaccgtcat agggatcggg caattcgctg tctgcgggaa agcgcaccac
    26941 atcacccgcg cgggcaatcg cttccagccg tggatgacct tccagcgcaa agcgtctacc
    27001 gagtacatcc ttcgccagcc cgtcgatggc aagcggaata aactgccgcg agtcgtaacg
    27061 tagcaacgca gacgcatcgc actccagcac ctgacgcagc gtggtgatca ggcgctgaaa
    27121 acgatcctga tgaccaatcc cacgctgcaa ttcgatggcg atattcgcca gcacatcaac
    27181 ggaaaaactc atctttgcct cactgtcaat ttgactatag atattgtcat atcgaccatt
    27241 ggattgatag tcattttgac tactcattaa tgagcataat tttatttata gagtaaaaac
    27301 aatcagataa aaaactggca cgcaatctgc aattagcaag acatcttttt agaacacgct
    27361 gaataaattg aggttgctat gtctattgtg gtgaaaaata acattcattg ggttggtcaa
    27421 cgtgactggg aagtacgaga ttttcacggc acggaatata aaacgctgcg cggcagcagc
    27481 tacaacagct acctcatccg cgaagaaaaa aacgtgctga tcgacaccgt cgaccataaa
    27541 ttcagccgcg aatttgtgca gaacctgcgt aatgaaatcg atctggcgga tatcgattac
    27601 atcgtgatta accatgcaga agaggaccac gccggggcgc tgaccgaact gatggcacaa
    27661 attcccgata cgccgatcta ctgtacagcc aacgctatcg actcgataaa tggtcatcac
    27721 catcatccgg agtggaattt taatgtggtg aaaactggcg acacgctgga tatcggcaac
    27781 ggcaaacagc tcatttttgt cgaaacacca atgctgcact ggccggacag catgatgact
    27841 tacctgacag gcgacgcggt gctgttcagt aacgatgctt tcggtcagca ctactgcgac
    27901 gagcatctgt tcaacgatga agtggatcag acggagcttt ttgagcagtg ccagcgttac
    27961 tacgccaata tccttacgcc gttcagccgt ctggtaacgc cgaaaattac cgagatcctg
    28021 ggctttaact taccagtcga tatgatagcg acctctcacg gcgtggtatg gcgcgataac
    28081 ccgacgcaaa ttgtcgagct gtacctgaaa tgggcggctg attatcagga agacagaatc
    28141 accattttct acgacaccat gtcgaataac acccgcatga tggctgacgc tatcgcccag
    28201 gggattgcgg aaaccgaccc acgcgtggcg gtgaaaattt tcaacgtcgc ccgaagcgat
    28261 aaaaacgaaa tcctgaccaa tgtcttccgc tcaaaaggcg tgctggtcgg cacttcgacg
    28321 atgaataacg tgatgatgcc gaaaatcgcc gggctggtgg aggagatgac cggattacgc
    28381 ttccgtaaca aacgcgccag tgctttcggc tctcacggct ggagcggcgg tgcggtggat
    28441 cgtctttcca cgcgcctgca ggatgcgggt ttcgaaatgt cgcttagcct gaaagcgaaa
    28501 tggcgaccag accaggacgc tctggagtta tgccgtgaac acggtcgcga aatcgcccgt
    28561 cagtgggcgc tcgcgccgct gccgcagagc acggtgaata cggtagttaa agaagaaacc
    28621 tctgccacca cgacggctga cctcggccca cggatgcagt gcagcgtctg ccagtggatt
    28681 tacgatccgg caaaaggcga gccaatgcag gacgttgcgc caggaacgcc gtggagtgaa
    28741 gtcccggata acttcctgtg cccggaatgc tccctcggca aagacgtctt tgacgaactg
    28801 gcatcggagg caaaatgagc aacggcattg tgatcattgg ttcgggcttc gccgcccgcc
    28861 aactggtgaa aaatattcgc aaacaggacg ccagtattcc attaaccctg attgccgccg
    28921 acagtatgga tgagtacaac aaacctgacc tcagccatgt tatcagtcag gggcaacgtg
    28981 ccgacgacct tacccgccag acggcgggtg aatttgccga gcagtttaat ctgctcctgt
    29041 ttccgcaaac ctgggtgacg gatatcgatg ccgaagcccg tgtggtgaaa agccagaata
    29101 atcagtggca atacgacaag ctggtgctgg caaccggtgc cagcgccttt gtcccgccag
    29161 tgcctgggcg tgagttaatg ctgacgttaa atagtcagca agagtatcgc gcctgtgaaa
    29221 cgcaactgcg ggatgcccga cgcgtgttga ttgttggcgg tggtttgatt ggtagcgaac
    29281 tggcgatgga tttttgtcgt gcaggcaaaa tggttacgct aatcgacaac gctgccagta
    29341 ttctggcgtc gttaatgcca ccggaagtaa gcagtcgctt gcagcatcgg ttgacggaga
    29401 tgggcgttca tctgctgttg aaatctcagt tgcaggggct ggaaaaaacg gattctggca
    29461 ttctggcaac gctggaccac cagcgcagca tcgaagtgga tgcggtaatt gccgccaccg
    29521 gactgcgccc ggaaaccgcc ctggcacgac gcgccgggct gacgattaat cgcggcgttt
    29581 gcgtcgatag ttatctgcaa accagtaatg ccgatattta cgcgctgggc gattgcgcgg
    29641 aaattaacgg tcaggtattg ccgttcctgc agccgattca acttagcgcg atggtgctgg
    29701 caaaaaatct tctcggcaat aacacgccgc tgaaactacc ggcgatgctg gtgaaaatca
    29761 aaacgccgga attaccgctg catctggcag gcgaaaccca gcgtcaggat ttacgctggc
    29821 agattaatac cgaacgccag ggaatggtgg cgcgcggtgt tgacgatgca gaccaacttc
    29881 gcgcctttgt ggtcagtgag gatcggatga aagaggcatt tggattgttg aaaacgttgc
    29941 cggtgtaggt gggctactgt gcctaaaatg tcggatgcga cgctggcgcg tcttatccga
    30001 cctacgggga cgcatgtgta ggccggataa ggcgtttacg ccgcatccgg caatggtgtc
    30061 caaatgcaac acgttttatc cgttctggac ttcacccgct aaccaacgcg ccgcagcaat
    30121 aaccccctgc cccagagaca aaccgccatc acccgccggt aaactctgtg gaaacagcaa
    30181 tgtgaaatca gcgagataat gcgccagacg tgcacgcagc aaacggttat gaataacccc
    30241 gccgctaaat accagcgtag tgataccaag catcgtggcc tgctcacgca tcaacgcggc
    30301 aaaaccctgc gccagcgcat catgaaacgc ccacgcgcgt tgattaaccg gtgcctgcca
    30361 gttcagccac tgctgccaga aagtggcgag atccagttga ttgtccaccc gcggcatcgt
    30421 caccggatgc gtcactccgt cgcatgaggc tgctagcgcc tccagagcac aagccgcttc
    30481 accttcataa cttaacgtgg ctggcgcaca gcccagtgcc gccgccacag catcgaacaa
    30541 acgcccacac gatgacgcca gcggcgcgtt aattccacgc tcaatggccc gcgccagtac
    30601 gctccagttt tgctgttgca cacttgccgt ttcagggtaa ttctgccact ccggcacaaa
    30661 gcgcaggcac tgcgccagca ggttacgcca cggctgcttc gccgccaaat cgccacccgg
    30721 aagcgccact gcaggcaagc cgcccaggtg ctcacattcg cgatagttca cccgcaggca
    30781 ctcgccgccc cacaaagcgc cgttctcccc cataccgata ccgtcgagcg tcaaagcaat
    30841 gacatcaccg ccatccagcg gccactgatg ctctgccaga cacgccgctg cgtgggcatg
    30901 atgatgcagc accgtttgcg tcggcagatt catttcgctc gcccactggc aggagacata
    30961 gcccggatgt gcgtcatgca caacgtattg cggagtgaaa ttgtagatgt tttgcatcag
    31021 gcgtaacgct tcgcgccact gcgtctggat gccatcgtca cttaaatcgc ccagatgctg
    31081 gctcaacacc acttgttcac cgcgcaccag gcagaaggta tttttcagat ccgcgccgag
    31141 acacagtaca ggcggaacat ttttaaagcc cggaggcaaa gccagcgcat ccggcacata
    31201 cccccgcgaa cggcgcagca tttcgccgct ttcgcgcacc accgaatcat ccatccgctg
    31261 cacaatgtcg cggttgtgta ttaagaatcc gtcggcaatt ccctgcaaat cctccagcgc
    31321 ctgttcgttg ctgatagccg gtggtttacc gctcaggttg ccagaggtca tcaccagcgg
    31381 gcattgtagt tcctgtaaca gcaaatgctg aagcggattc gcaggcaaca ttaccccgac
    31441 ttcgttaagg ccaggggcga tatcatcaca aagctcagga acgtattttt tatccaccag
    31501 cacaatcggc gcggcgggcg tggtaagcaa ctggcgcgca gcgtctggta aaccgtcagc
    31561 cactggcaac atgactgcca gcggtttcgc cgggcgatgt ttgcgcgccc gaagtgtcgc
    31621 caccgcgtta ctgttacgtg catcgcaggc aagatgaaat ccgccaatcc ctttgatggc
    31681 gacaatgttg cccattttta actgtgcgat agccgcctgt aatgccgcct cttgttctgc
    31741 atgttcacca tgacttaccc attcaagata cgggccacac tccgggcagg ccaccggctg
    31801 ggcgtggaag cgacgatcga gcgggtcgcg gtactcttta tcacaggctg gacacagcgg
    31861 aaacgccgcc atcacggtaa acgggcgatc gtaaggcatg gcgcgaataa tggtgaagcg
    31921 cgggccgcag tgggtacagt tgataaatgg ataacgataa cgccgttcgc ctggggtatt
    31981 catttcggca aggcaagcag ggcaagtggc ggcatcggga acaatttgcg tattcatggc
    32041 gccgcccgcg ctctggcgga tggtgaactc agtgggcagt tgtgaccaga taaacggttc
    32101 acgctcaacg ctatcaatac gcgccagcgg cgggcagtgc tgatgcaatt gaacaagaaa
    32161 cgtttccggg tcttccagca gccggacttc tacgccatcg ccgtcattac agacatcgcc
    32221 gtgaagattt aattgctgtg ccagctgcca gacaaacgga cgaaaaccga cgccctgcac
    32281 tttgcctcga atacgcagtt ggacaccgca agatgtgttt tttgccattg agttattccc
    32341 gccatcatga attgcgtaac ccgtccagcc ggacacgaca gcgtcgcatc cggcagtcac
    32401 aggtcggcga taccgcccgc tccgtattct acgaatattt ccgggaattc ctttgatgcc
    32461 agaacagttc tgtaagattt ttagaacatc agcgccgtac ggcggcgttt ttctgcgctc
    32521 agttgttcaa gtttattgcg atcgacacaa attagcgcat gggtcgggca agccgccata
    32581 cacgccgggc cgtcttcacg atggttgcac aggtcgcatt tattggcttc ggctttgtca
    32641 gcccgtacat tcagccctgc gccgctgttg cgaatcaccg gacgtaccac cacttccatc
    32701 gcgccatacg ggcaagccac aacgcaggtt ttgcaaccaa tgcaacgttc ctgcatcaca
    32761 tgaacaaacc ctttatcacg gctgatagca ccattcgggc agacgttagc gcacggtgca
    32821 tcttcacact gacggcaaac tgtcgccgtg gaaatgttca cacctttaat gacatggata
    32881 cgcggtaaaa atgtttccgg ggtcagcgat gcacagtcct gattttcctg atgagaaacc
    32941 acgcacgcca cttcacaggt acggcaacca atacatttac tcgcgtcagc aatgatgaaa
    33001 cggttcatca aattctccag caatgacagt taatgcgccg atacattcac aaatcatgcc
    33061 agtttttaat ttgctgttat ttaaggaaat taatttctgt aatgcaggaa aaaagatgtc
    33121 atcgacacta gtgacgatga catgtgatga caatgtttat cgcgaaagag caatgagtga
    33181 gtcgcggcgg atcagttttc cgctgaaggt tttcggcggt gagaaatccc cgccatcgag
    33241 cataaaaatc agccgtccaa taatttcctg aatcatctca gtcaccggaa tttttacgct
    33301 ggagagcgcc ggaacgatat agggggcaat tgcgatatca tcgaatccga taactgacac
    33361 ctgctctggc accgctacgc cgcgcttgtg taacgctttc atcgcaccta tcgccatatc
    33421 gtcgttactg gcaactaacg cgctaaattt agccccacgt tcgagcaacg tttctacccc
    33481 ttcggccccg ctggcgggcg tccatttacc gttagcgata agtttttcat tgagcgcaat
    33541 accatgctgc gccagcgcgt ctttatatcc ggcaagacgt tcaacgctgg tgggggaatc
    33601 catcgagccg gtaagaaaag caatctcctg atgcccggcg tttatcaact ctgccacggc
    33661 gttaaagctg gtctgtttat gatcgcacca gacgctatgg ctgctatttt tgcgcaggcg
    33721 gcgattaagc accattatcg gctgactgtg cgcgtcaatg atgtcatcga tctcatccac
    33781 acttaaaaaa cgcgggtaaa tcataatggc gtcgcagcgc aaatccagca gatactgaat
    33841 cgcctggcgc tcttcttcgg cgctgtgttt accatctgcc aatagcaact gccgcccttt
    33901 ctcttccgcc attcgcgcgg catgaaacag taattcacta aaataaatgc cgtggtaaag
    33961 cgtgttagtc actaccagcc ccagcgtctg ggtacttttc gccgacagat tgcgcgccag
    34021 caagtttgga cggtaaccgc tctcttctac cgcctgaaac acgcgatctt tagtctcctg
    34081 gctgacgtag ccattacctg aaagcacgcg ggaaacggtc gcttttgaaa ccccggcgcg
    34141 cttcgccact tccagcatcg tcgtcatcat tttcatccct ttacacgcaa tcaacgcagt
    34201 gtactgcacc gtttaccgat tgtccttgcg caatcggcgg gaaaaatatt caggtgaccg
    34261 gtttcacaaa tataaaaaat gaacaattaa ctctcttgct tatttggtta caactattca
    34321 tgattttgtg aaaccggttt cttaattccg tttcagcatc ggcatttttc cgtcacgtcg
    34381 actgataaca actacatctt ccctactgat aacaggataa aatccgatgg ccaaaaatta
    34441 tgcggcgctg gcacgctcgg tgatagcggc actgggcggc gttgataaca tctcggcggt
    34501 cacgcactgt atgacgcgtt tgcgctttgt tatcaaagat gatgcgctta tcgacagccc
    34561 gacgttaaaa accatccccg gcgtgctcgg cgtggtacgt agtgacaacc agtgtcaggt
    34621 gattattggc aataccgttt cacaagcctt tcaggaagtc gtcagcctgc tgccgggaga
    34681 tatgcagccc gccctgcccg tgggtaaacc caaactcacg ctgcgtcgca ttggtgcggg
    34741 gatcctcgat gcgctgatcg gcaccatgtc accgctgatc ccggcgatta tcggcggatc
    34801 gatggtcaaa ctgctggcga tgatcctcga gatgagcggc gtgctgacaa aaggatcgcc
    34861 gaccttaacc attctgaatg tgattggtga cggtgctttc ttcttcctgc cgctgatggt
    34921 cgcggcttcg gccgccatca aatttaaaac caatatgtcg ctggcgattg ccatcgcggg
    34981 tgtgctggta catccgagtt ttattgaact gatggcgaaa gcggcccagg gtgaacatgt
    35041 cgaatttgcc ctgattccag tcaccgcggt gaaatacacc tacacagtga tcccggcgct
    35101 ggtgatgacc tggtgcctgt catatatcga acgctgggtg gatcgcatta caccggcggt
    35161 gaccaaaaac ttcctcaagc caatgctgat tgtgttgatt gccgcaccgc tggcaatcct
    35221 gctgattggc ccgattggta tctggatcgg tagcgccatt tcggcgctgg tttacaccat
    35281 tcatggttat ctgggctggc tttcagtcgc cattatgggc gcgctgtggc ctctgctggt
    35341 aatgaccggg atgcaccgcg tctttacgct aaccatcatt cagaccattg ccgaaaccgg
    35401 caaagaaggg atggtcatgc cgtcagagat cggcgctaac ctgtcgctgg gcggttcatc
    35461 actggcggtg gcgtggaaaa cgaaaaaccc ggaactgcgc cagactgcgc tggctgcggc
    35521 ggcatcagcc attatggcgg ggatttccga accggcgtta tacggcgtgg cgatccgcct
    35581 gaaacgtccg cttatcgcca gtcttatcag cggttttatt tgcggcgcgg ttgccggtat
    35641 ggcggggctt gccagccact caatggcagc gccggggcta tttaccagcg tgcagttctt
    35701 cgatccggcg aatccaatga gcatcgtctg ggtgttcgcg gtcatggcgc tggcggtggt
    35761 gctgtcgttt atcctcacac tgttgctcgg ctttgaggat attcctgttg aggaagcggc
    35821 tgccgaggcg cgaaagcatc agagcgcaca accgaccgtc gccaaagaag taagtcttaa
    35881 ttgaggatga aaatgtcagt atttccagaa ggttttttat ggggcggcgc gcttgccgcc
    35941 aaccagtctg aaggtgcgtt ccgtgaaggt ggcaaaggac tgacaacggt cgatatgatc
    36001 ccacacggcg agcatcgaat ggcggtgaaa ctggggctgg aaaaacgttt tcagttgcgc
    36061 gatgacgagt tttatcccag ccatgaggcg acgggttttt atcatcgtta taaagaagat
    36121 atcgccctga tggcagagat gggattcaaa gtgttccgta cctcaattgc ctggagccgc
    36181 cccttcccgc agggtgatga actgacgccc aaccagcagg gcattgcttt ttaccgttct
    36241 gtctttgaag agtgtaaaaa gtacggtatc gaaccgctgg tcacattgtg ccacttcgat
    36301 gtgccgatgc agctggtcac cgaatatggc tcctggcgta accgcaagct ggtggagttt
    36361 ttcagccgct acgccaggac ctgctttgaa gcatttgatg gtctggtgaa atactggcta
    36421 accttcaatg aaatcaacat tatgttgcat agcccgttct ccggcgcggg gctggtgttt
    36481 gaagaaggtg aaaatcagga tcaggtgaaa tatcaggccg cgcatcacca gctggttgcc
    36541 agtgcgctag ccaccaaaat cgcccatgag gttaacccgc aaaatcaggt gggctgtatg
    36601 ctggcgggcg gtaacttcta cccttacagt tgcaagccgg aagatgtctg ggcggcgctg
    36661 gagaaagatc gggaaaacct gttttttatc gatgtgcagg cgcggggcgc gtatccggct
    36721 tactctgctc gcgtattccg cgaaaaaggg gtaaccatcg acaaagcacc gggcgatgat
    36781 gagatcctga aaaacaccgt cgattttgtc tctttcagct attacgcctc gcgctgcgcc
    36841 tcggcggaga tgaacaccaa caatagcagt gcggcgaacg tagtgaaatc gctgcgtaac
    36901 ccgtatctac aggtgagcga ctggggctgg ggaattgatc cactcggtct gcgtatcacc
    36961 atgaacatga tgtacgaccg ctatcagaag ccgctgtttc tggtggaaaa cggcctcggc
    37021 gcaaaagatg aacttgctgc caatggcgag attaacgatg actatcgcat cagctattta
    37081 cgcgaacata tccgcgcaat gggcgaagcg attgcagatg gcattccgct gatgggctac
    37141 accacatggg gctgtattga tttagtttcc gcctctacgg gtgaaatgag caaacgctac
    37201 ggttttgtct ttgttgaccg tgacgacgca ggcaacggca cgctgacgcg cacacgtaag
    37261 aaatcgttct ggtggtataa aaaagtgatt gccagtaatg gggaagattt agagtaggta
    37321 acctactgtc gcactaacca ggaaggcccg ataagcgttg cgcatcgggc aatctggggt
    37381 ttgctatcag ttctgattcc ctcatcaaga acatccctgt cctgattact tattgaaaaa
    37441 ctactcttct tccaccgcta actgcgcgaa gccgccgttc ccttcccagc cttccagtcg
    37501 ttgataaacg gtttctaccg catctttaat cggctgggtc atcgggtagt aaaagccgac
    37561 gatatccggc tggatgccga ggaaaatcac ttcgccaata tcttctttca actgatcgat
    37621 aaggtagttc aacggcatgt tatgggtagt catcataaac atctcggcga tatcatccgg
    37681 gtcgatgatg cggatttcgc cggggtttag ccccatatcc gtggcgtcga caatcagcag
    37741 ccgtgtcggg cgcagttcgc ggatagcgac aatgtcgttt tccggtgcgc taccgccgtc
    37801 aatcaccacc cagttacctt tcggcgcggc ggcgcacttt tccgccagca gcggacctgc
    37861 gccatcatca cccatcatgc tattgccaac acagagtaaa acgtcagtca cgcagtctcc
    37921 tcaccatcaa atagatggcg ttttcctgat gaatatcatg cagcatactt aacagcattg
    37981 tactccattc ctgctgctgc gaagtttgcg ctgcccgggc tttatcgaat gcattggcca
    38041 gcatcggcac atggttaatg tcgatgacga tctcaccata tttcggcaca ccttccattt
    38101 tgcggcgggc ttcgctgccc gcctccagag tggcaatcca tgccagatat tcatcccacg
    38161 ggcaggtgag cgccgcttcc aggcaatcga taaccccaag gtggtgacca atcgccaggc
    38221 tgtaatagac cacctgctgc gcctcggcgg gcgtggcatc gttctcatca ataaatttac
    38281 ggctcagttg actgaacacc accttttcac tcatcggata cgcgcctctt caacaacatg
    38341 attcagatgg ctgacaatct cgttcagacg cggatcgttt tccgcttcca gccagcgtgc
    38401 aacctgttct tcgccctgcc ctaactgtgt aaggtaatca tcggcaatct gacgaccgta
    38461 acgataaccc gccagtcgac gtgcttcgcg atccactttc acacgcagcg gctgcaccat
    38521 atcgccatgc aggatctccg ccggttgttc atcctgctca cccggcccac gggcgtgaat
    38581 tttctgctcc agcaggccga gcgccattgc aaagccgtac agcgtggcgg ctggcgttgg
    38641 cgggcagcca gggatataaa catccaccgg gacgatttta tccgtaccgc cccacacgca
    38701 gtagagatcg tggaagatcc cgccgctgtt accgcaggca ccgtaggaga tacaaatttt
    38761 cgggtccggc gcggactgcc acgcacgcag cgcaggggat cgcattgcac gggtgaccgc
    38821 gccggtaaac agtaaaatat ccgcatgacg cggtgaagga acgactttaa tgccgaagcg
    38881 ttctgcatcg aacagcggcg acaaagtggc gaaaatttcg atttcgcagc cgttgcagcc
    38941 gccgcagtcc acgcggtaaa cgtaggcaga acgtttgatt tttttcagta acgacgcctt
    39001 catgctggcg atggattcat ccaccgtcat cgggaccgga atgccgttgg cgtcacgggg
    39061 gcctaataaa ttgctcatca gatggcctct ttcatatggc gagtcagttc aatacggtcg
    39121 gacggcacca ggcatttctg gcgcttacat tccgggcaag tctcaaagct ttcgcggtgg
    39181 ttttccgcgc ggctgtcgcc attgtgctta agcagcgcaa tggcgtagtc gatctctttc
    39241 tggacggcga aaggacgatt gcagacgcgg cagttgcaca gcgcgaagcg ggactgttgc
    39301 aggaagtctt ctttcttcca caccgccagt tcgtactctt gcgacagttt gattgccgcc
    39361 gtcgggcaga cttcttcgca gcgtccgcag aagatgcagc gcccgagatt gaactcccac
    39421 gccagctcac cggtggcgag gtcagtttca accgttaagg cgtttgacgg gcaggcattg
    39481 acgcaggccg cgcagccgat gcactgctgc gggttctgct ctggcttacc acggaagttt
    39541 ttatcaaccg caatcggttc cagcggataa gacgaggtcg ccgtgccggt tttgatgact
    39601 tttttgataa aggtaaacat ggcgattcct tatttcagcg gcgagttttt acgctcaatg
    39661 ctgtaacgct cgagttcttt gtacggcacc actttgctct ttttcttacg cacatcgacc
    39721 acggtcatgc ggtcggtaca ggagtagcaa gggtccagac taccgataat cagcggcgca
    39781 tcagaaacgg tgttgccgcg cagcatgtag cgcagggtcg gccagttcgc gtaggtcgcg
    39841 gcacggcagc gccagcggta cagcttctgg ttgtcgccgg tcatgctcca gtggatatca
    39901 tcgccgcgcg gcgcttcggc aaagcccagc gcaaaacggt gcggaatgta ggtaaagcct
    39961 tccaccatca gcgggccgcc cggcaggtta tccagaccgt agtcgatcat gttcagcgcg
    40021 gtatagactt cgttaatacg cactttcaga cgcgaaataa cgtcgcagcc ctgctcgctg
    40081 tggacttcca ttggcagcag gccataacca acaaacgggt gatcggcgcg ggtatcgcgg
    40141 gcgtgaccgc tggcacggac catcgggccg acgttactga agtcgcgagc gatttccggg
    40201 tccagacgac caatgccgac agtgcgctgt tccatgttcg gcgtactcag cagcacatcc
    40261 accagctcct gcacttcacg acgcatctgt tgcgccagtt gacgggtctg gatcatgtca
    40321 tctttcagca gatcgcgacg aatcccgccg atcaggttca ggccgtaggt tttacgcgca
    40381 ccggtaagga tctctgccat tttcatggag gtttcacgca cgcggaagaa ctgcataaag
    40441 ccggagtcaa agccggtaaa gtgacaggcg aggccgaggt tgagcagatg cgagtgcaga
    40501 cgttctacct ccagcagaat ggcgcggatc atctgcgcac gttctggcac ctgaataccc
    40561 atcgcgtttt ccaccgacgt ggtgtaggcg gtgctgtgag caaaaccgca gatcccgcac
    40621 acacggtcag agaggaaggt cacttcgtta taacccatgc gggtttctgc cagtttttcc
    40681 atgccgcgat ggacatagaa caggcggtag tcggcgtcga taatgttttc gccatcgacg
    40741 aacagacgga agtggcccgg ttcgtcagaa gtgacgtgca gcggaccaat cggcacgacg
    40801 ttgtttttct tgtcgcccag ttcgttgatg aactcgtagg tttcagcatc ggtggtcggt
    40861 gccggacgct gacgataatc catgctgtct ttacgcagcg gataaagttc atccggccag
    40921 tcatccggca gcaccagacg acgttcatcc ggcaaaccaa ccggaatcaa accgtacata
    40981 tcacgtactt cacgctcgcc ccacaccgca gccggaacgc gcggcgtcac ggaaggatac
    41041 tccggtttgt tggcatcaac ttcgacgcga accgttaccc agcacttagt ccccttctcc
    41101 atcgacagca cgtagtaaac ggcgtaatga ccattcagtt tgcgttcgtc gttaccaaaa
    41161 agcaccgaca gccagccccc ctgcttgtag tagagaaact ccaccacttc cggcagatag
    41221 ttcaccttta cggtgacagt cagctgatct ttggtctgcc aggcgtggtc cagcacgacg
    41281 cccggaaatg cctcattcag cgcggcgaga taatgttgac ctaatttttc ttcagacatg
    41341 ctcaaactct ctttaatcac gccgccagca aggagacgaa cgctaaaaat gcaaagccaa
    41401 acccagccca ggtaatgcgc ggagtaatat caagacgcag acgggccatg ctgttttcga
    41461 acagcgcgat aaccaggacg ccgaccacca gtttgacgat ggcaatcacc aacgccagca
    41521 gcagcccacc gacggtgaag gtttccattt gtccccacgg aataaacacc ccgacgaaca
    41581 tctgcagcac caccaactgt ttcaggctga taccccattt catgacgcca aagccgctgc
    41641 cgctgtactc agagagcgga ccttcctgca actcctgctc ggcttccgcc aggtcgaacg
    41701 gcagtttgcc catttcgata aaagtggcga acgcacaggc acaaagcgcc agcaccagcg
    41761 ggatgctctg gctcagcggc cagtgataaa cggtgtcggt gatgttgctg atgttggtgg
    41821 aactggcaac ctgtgcggca acccacagac caagcagcag catcggttcg accagcacgc
    41881 caagcatcgc ttcacggctc gcgccgatag cggtaaacgg actaccggta tccagaccag
    41941 aaatggcaaa gaagaaacgc gcgatggcaa agagatacag taaggtgatc aaatcaccca
    42001 gttgcggcag cggagaaccg acggtcacca ccggcagcgc agtagcgata gtcagcatga
    42061 cgcccaccat cacatacggc gtcaggcgga acacccagcc ggaggcatcc gggccaacgc
    42121 tctggcgacc cagcagtttg ataatgtcgc gatactcctg caacacgccc ggcccgcgac
    42181 ggttatgcaa gcgggcgcgc gccacgcggg taataccgga gagcagcggc gcaacggcaa
    42241 atagcaccag cgcctgaatt aacggatata aaacactcat tctcaggctc ctcgtgaaac
    42301 aataatcacc accagcaccg ccagttcaac cagcgccatc cggcggaaca gcaacgcact
    42361 gccttcgcac tgccagcccg gcaccagcga caccggattc agccatttac gcagtttcag
    42421 caccggcgca aacgcctgtt tcaccggcat ggcaaaaccg tgggcggtaa ttaccattga
    42481 tttttcatgg tcgtagccgc acacccaggc cgcgccgcgg gaacgcgatg gcaaacgatc
    42541 gcctttgcaa atcgccataa tgatgaatgg cagcagcggg caggcaatca gcagcaacgt
    42601 gatcatcggt tgagaaacgg tggtgttagc aggttccagc ggcagaggta cagcagcaga
    42661 gagcatcggc agcagccacg gcgcagcaac accgccaatt acgcagcaaa tcgccagtgc
    42721 cactacgctt acgctcatca ggagcggtgc acaggtggcg ttttcggctt ctttggtacg
    42781 cggcgcgccg aggaacgtga cgccatagac tttcgccata cacatcaccg ccagcgcacc
    42841 ggtaattgcc agacccacag cgagcagcgg ccccagcaga cgggcaacaa acgcgccact
    42901 attgctcagt ttgaagaagg attgatagat aacccattcc ccggcaaaac cgttcagcgg
    42961 cggcagtgca gccattgcca tcagcccgac taacatagcg atggagataa ccggcatttt
    43021 cttgccaata ccaccgagtt tttcgatatc gcgatgaccg gtacggaacc agacgctccc
    43081 cgccccgagg aacagtacgc ttttgaacag gctatggtta agcagatggt acagaccacc
    43141 gaccaggcca agagcaatca gcgccggttg ttcgagcgcg atacccgtta cgccagcgcc
    43201 cagccccagc aggataatgc cgatattttc cagggtgtgg taagccagta gacgctggat
    43261 attatgttcc atcaacgcat acagaccgcc gacaaacgcg gtgatcatac cgagcaccag
    43321 cagcgcgatc ccccaccaca gcggtgcatt accgcccagc agtgacaggg ttaaaatgcc
    43381 cagcaggcca attttcatga ctaccgtaga aaacaacgcg gcagctggcg cagaggcgtt
    43441 cgcatgtgcc tgcggcaccc agccgtgcag cggaataatc ccggccagca ggccaaagcc
    43501 aatcacactg agcagccaga tatcggaacc gagcggcagc tgttgcatac gcatatccag
    43561 caggcgcaga tccagcgtgc cgtaacgctg ccacagcagc cagcaagcaa tcgccagcag
    43621 cagagtgcca agacgcccca gcgcaaacca cagtttgccc tctttgctgt tgctggtgag
    43681 gaacaccgca cacaaggcca tgacttcggc cattaccacg aacatgccga ggttgctggc
    43741 aatgacggcg cagacggcgg cagccatcaa catattgatc tgcaagccgt tgcacttcac
    43801 ctgcgcgtgg cgatgccagt caatgttgta gaggctgaca aacagaccgc acagaccgag
    43861 cgtaatcagc caaatcgcgt taagcggaga gatttgcaca tcgtagctta ccagcgacag
    43921 cgcaccgctc acgccaaccg cgccagtcag tacagtgaag cccgcggctg ccgtatacag
    43981 actaccaacc gcgccgccaa ttccggctat ccagccgctt agcgcctttt gaaaagaaaa
    44041 gagaaatgcc agaacagcgg cggcgacaaa ccacgccacg ccgctattga tcagggaaat
    44101 tgcgctcatt tagcctctcc actttgagcc tgctgaaaca aggtgagatc gccaaagtcc
    44161 gtgttaaagg tcagctcacg cttacgtttg ctgacgcggg cgatatcggt gttatccacc
    44221 agatgcaggg ctttagtcgg gcacatccgc acgcaggccg gaccttgttc atcaaagcta
    44281 caaaggtcac atttgacggc gatcgcgcga atacccggca cccagtcaag cagcgtactg
    44341 atacgcgccg gagcaggcgg tgccggtggc gctttcgggg tattggcatt tgcaggaata
    44401 tccagcggac ggctgccgga aaattcaatt gcgccaaacg ggcaggcgat gccgcacagc
    44461 ttgcagctta cgcacaggct ttcattcaac tgcacggccc catcaacgcg ggtgatggcg
    44521 ttaaccgggc agaccaccgc gcagggtgca tcttcacagt ggtgacagag ctgcggcgca
    44581 gattcttttt catttagcat cactctcagg cgcggcattg attgcaggcc gtgctggcga
    44641 tgcgtctctg aacaggcggc ctcacaagtg tggcaaccga tacagagcgt ggagtcagca
    44701 attacaaaac gattcaccag gcattcctca ggtgattgtc atttttgacg aaaacatgcc
    44761 gatgaaatgt cattttcgac actcatcgac acgcccatcc ccaaacaggc gtaacgcctg
    44821 caaaacgggc aaagcctcag ctcatgctgc cgggctttgt ccctttacca gttggcttaa
    44881 attcaccggc acattgtttt caatagcggt gtataagctg tcgtaaacgc cagcgatttt
    44941 ttcgagatag tgttccagaa cctgttcgcg atcgcggatg ttgaccttgc catcaatcat
    45001 gccgtcaatg ctcagttgcg cctgcgcaat ccgctgtttg tcttcgccat cttttgtttc
    45061 gacgtaatac tcgatggtgt ggctgttaaa gccatccgcc agggtgacgt aaatacgaaa
    45121 atgttcaaaa aggacgaaac agagttcttt gtccggcgcg cagctcatgg cgtgatgcag
    45181 gcgcgctttc gataacgtga tcccctgaac cagcgaattg cagtagatgt gccactggtc
    45241 ctgtaggcga cgatgccgct gtgcgatgta atcggctttc tcgcttattt cccaaatagt
    45301 cattgtcagg ttacccgttt aacagagatg ccagctttaa gcattttttg tgccaacttt
    45361 taatttatta ttattaaagc gatttttaag attttggtga atttcgtcgc cgtgtcgacg
    45421 tgtcatttcg acatcatcga cattattcac tgcagggata atcaacactg gcacaattat
    45481 tgcttgtagc tggcaatagt taatgggagg cgatatgcac gaaataaccc tctgccaacg
    45541 ggcactggaa ttgatcgaac agcaggccgc aaaacacggc gcaaaacgcg taactggggt
    45601 ctggctcaaa attggcgcat tttcttgtgt cgaaaccagc tctcttgcct tttgttttga
    45661 tctggtttgc cgcggcagcg tggcggaagg ttgtaaactg cacctcgaag aacaagaggc
    45721 cgaatgctgg tgtgaaacat gccaacagta tgtgacgcta ctgacccagc gcgtccgccg
    45781 ctgtccacag tgtcatggtg acatgctaca gattgtggca gacgacggtt tacagattcg
    45841 gcggatagaa atagaccagg agtgagcgat gtgtacaaca tgcggttgcg gtgaaggcaa
    45901 cctgtatatc gagggtgatg aacataaccc tcattccgcg tttcgtagcg cgccatttgc
    45961 cccggcggca cgcccgaaga tgaaaatcac cggcattaaa gcgcctgaat ttacccccag
    46021 ccagactgaa gaaggcgacc tgcattacgg tcatggcgaa gcgggcactc acgcaccggg
    46081 catgagccag cgtcggatgc tggaagtcga aattgacgtg ctggacaaaa ataaccgtct
    46141 ggctgaacgc aaccgcgcgc gctttgctgc ctgcaagcaa ctggtgctca acctggtttc
    46201 cagcccaggt tccggtaaaa ccaccctgct gacggaaacc ttaatgcgcc tgaaagacag
    46261 cgttccgtgc gcggttattg aaggtgacca gcaaaccgtg aacgatgccg cacgcattcg
    46321 cgctaccggc acaccagcga ttcaggtgaa caccggtaaa ggctgccatc ttgacgcaca
    46381 gatgattgcc gacgccgcac cgcgtctgcc actggacgat aacggtattc tgtttatcga
    46441 aaacgttggc aacctcgtat gcccggccag cttcgatctc ggtgaaaaac acaaagtggc
    46501 ggtgctttcc gttaccgaag gtgaagacaa accgctgaaa tatccgcata tgtttgccgc
    46561 cgcctcgctg atgctgctca acaaagttga cctgttgccg tatctcaact ttgacgttga
    46621 gaagtgcatc gcctgcgccc gcgaagtcaa tccagaaatt gaaatcatcc ttatttccgc
    46681 caccagcggc gaagggatgg accagtggct gaactggctg gagacacagc gatgtgcata
    46741 ggcgttcccg gccagatccg caccattgac ggtaaccagg cgaaagtcga cgtctgcggc
    46801 attcagcgcg atgtcgattt aacgttagtc ggcagctgcg atgaaaacgg tcagccgcgc
    46861 gtgggccagt gggtactggt acacgttggc tttgccatga gcgtaattaa tgaagccgaa
    46921 gcacgcgaca ctctcgacgc cttgcaaaac atgtttgacg ttgagccgga tgtcggcgcg
    46981 ctgttgtatg gcgaggaaaa ataatgcgtt ttgttgatga atatcgcgcg ccggaacagg
    47041 tgatgcagtt aattgagcat ctgcgcgaac gtgcttcaca tctctcttac accgccgaac
    47101 gccctctgcg gattatggaa gtgtgcggtg gtcataccca cgccattttt aaattcggcc
    47161 tcgaccagtt actgcctgaa aacgttgagt ttatccacgg tccgggttgc ccggtgtgcg
    47221 tactgccgat gggcagaatc gacacctgcg tggagattgc cagccatccg gaagtcatct
    47281 tctgtacctt tggcgacgcc atgcgcgtgc cggggaaaca gggatcgctg ttgcaggcaa
    47341 aagcacgcgg tgccgatgtg cgcatcgtct attcgccgat ggatgcgttg aaactggcgc
    47401 aggagaatcc aacccgcaaa gtggtgttct tcggcttagg ttttgaaacc accatgccga
    47461 ccaccgccat cactctgcaa caggcgaaag cgcgtgatgt gcagaatttt tacttcttct
    47521 gccagcatat tacgcttatc ccgacgctgc gcagtttgct ggaacagccg gataacggta
    47581 tcgacgcgtt cctcgcgccg ggccacgtta gtatggttat cggcactgat gcctataatt
    47641 ttatcgccag cgattttcag cgtccgctgg tggtggctgg tttcgaaccc cttgatctac
    47701 tgcaaggcgt ggtcatgctg gtggagcaga aaatagcggc ccacagcaag gtagagaatc
    47761 agtatcgtcg ggtggtaccg gatgccggta acctgctggc gcaacaggcg attgccgatg
    47821 tgttctgtgt caacggcgac agcgaatggc gcggcttagg cgtgattgaa tcttctggcg
    47881 tgcacctgac gccggattat caacgattcg atgccgaagc acatttccgc ccggcaccgc
    47941 agcaggtctg cgatgacccg cgcgcgcgtt gtggcgaagt cttgacgggc aaatgtaagc
    48001 cgcatcaatg cccgctgttt ggtaacacct gtaatcctca aaccgcgttt ggtgcgctga
    48061 tggtttcctc cgaaggagcg tgcgccgcgt ggtatcagta tcgtcagcag gagagtgaag
    48121 cgtgaataat atccaactcg cccacggtag cggcggccag gcgatgcagc aattaatcaa
    48181 cagcctgttt atggaagcct ttgccaaccc gtggctggca gagcaggaag atcaggcacg
    48241 tcttgatctg gcgcagctgg tagcggaagg cgaccgtctg gcgttctcca ccgacagtta
    48301 cgttattgac ccgctgttct tccctggcgg taatatcggc aagctggcga tttgcggcac
    48361 cgcgaatgac gttgcggtca gtggcgctat tccgcgctat ctctcctgtg gctttatcct
    48421 cgaagaagga ttgccgatgg agacactgaa agccgtagtg accagcatgg cagaaaccgc
    48481 ccgcacggca ggcattgcca tcgttactgg cgatactaaa gtggtgcagc gcggcgcggc
    48541 agataaactg tttatcaaca ccgcgggcat gggcgcaatt ccgacgaata ttcactgggg
    48601 cgcacagacg ctaaccgcag gcgatatatt gctggttagc ggtacactcg gcgaccacgg
    48661 ggcgactatc cttaacctgc gtgagcagct ggggctggat ggcgaactgg tcagcgactg
    48721 cgcggtgctg acgccgctta ttcagacgct gcgtgacatt cccggcgtga aagcgctgcg
    48781 tgatgccacc cgtggtggtg taaacgcggt ggttcatgag ttcgcggcag cctgcggttg
    48841 cggtattgaa atttctgaat cagcgctgcc ggttaaacct gccgtgcgcg gcgtttgcga
    48901 attgctggga ctggacgccc tgaactttgc caacgaaggc aaactggtga tcgccgttga
    48961 acgcaacgcg gcagagcaag tgctggcagc gttacattcc catccactgg ggaaagacgc
    49021 ggcgctgatt ggtgaagtgg tggaacgtaa aggtgttcgt cttgccggtc tgtatggcgt
    49081 gaaacgaacc ctcgatttac cacacgccga accgcttccg cgtatatgct aataaaattc
    49141 taaatctcct atagttagtc aatgaccttt tgcaccgctt tgcggtgctt tcctggaaga
    49201 acaaaatgtc atatacaccg atgagtgatc tcggacagca agggttgttc gacatcactc
    49261 ggacactatt gcagcagccc gatctggcct cgctgtgtga ggctctttcg caactggtaa
    49321 agcgttctgc gctcgccgac aacgcggcta ttgtgttgtg gcaagcgcag actcaacgtg
    49381 cgtcttatta cgcatcgcgt gaaaaagaca cccccattaa atatgaagac gaaactgttc
    49441 tggcacacgg tccggtacgc agcattttgt cgcgccctga tacgttgcat tgcagttacg
    49501 aagaattttg tgaaacctgg ccgcagctgg ccgcaggtgg gctataccca aaatttggtc
    49561 actattgcct gatgccactg gcggcggaag ggcatatttt tggtggctgt gaatttattc
    49621 gttatgacga tcgcccctgg agcgaaaaag agttcaatcg tctgcaaaca tttacgcaga
    49681 tcgtttctgt cgtcaccgaa caaatccaga gtcgcgtcgt taacaatgtc gactatgagt
    49741 tgttatgccg ggaacgcgat aacttccgca tcctggtcgc catcaccaac gcggtgcttt
    49801 cccgcctgga tatggacgaa ctggtcagcg aagtcgccaa agaaatccat tactatttcg
    49861 atattgacga tatcagtatc gtcttacgca gccaccgtaa aaacaaactc aacatctact
    49921 ccactcacta tcttgataaa cagcatcccg cccacgaaca gagcgaagtc gatgaagccg
    49981 gaaccctcac cgaacgcgtg ttcaaaagta aagagatgct gctgattaat ctccacgagc
    50041 gggatgattt agccccctat gaacgcatgt tgttcgatac ctggggcaac cagattcaaa
    50101 ccttgtgcct gttaccgctg atgtctggcg acaccatgct gggcgtgctg aaactggcgc
    50161 aatgtgaaga gaaagtgttt accactacca atctgaattt actgcgccag attgccgaac
    50221 gtgtggcaat cgctgtcgat aacgccctcg cctatcagga aatccatcgt ctgaaagaac
    50281 ggctggttga tgaaaacctc gccctgaccg agcagctcaa caatgttgat agtgaatttg
    50341 gcgagattat tggccgcagc gaagccatgt acagcgtgct taaacaagtt gaaatggtgg
    50401 cgcaaagtga cagtaccgtg ctgatcctcg gtgaaactgg cacgggtaaa gagctgattg
    50461 cccgtgctat ccataatctc agtgggcgta ataatcgccg catggtcaaa atgaactgcg
    50521 cggcgatgcc tgccggattg ctggagagcg atctgtttgg tcatgagcgt ggggctttta
    50581 ccggtgccag cgcccagcgt atcggtcgtt ttgaactggc ggataaaagc tccctgttcc
    50641 tcgacgaagt gggcgatatg ccactggagt tacagccgaa gttgctgcgt gtattgcagg
    50701 aacaggagtt tgaacgcctc ggcagcaaca aaatcattca gacggacgtg cgtctaatcg
    50761 ccgcgactaa ccgcgatctg aaaaaaatgg tcgccgaccg tgagttccgt agcgatctct
    50821 attaccgcct gaacgtattc ccgattcacc tgccgccact acgcgagcgt ccggaagata
    50881 ttccgctgct ggcgaaagcc tttaccttca aaattgcccg tcgtctgggg cgcaatatcg
    50941 acagcattcc tgccgagacg ttgcgcacct tgagcaatat ggagtggccg ggtaacgtac
    51001 gcgaactgga aaacgtcatt gagcgcgcgg tattgctaac acgcggcaac gtgctgcagc
    51061 tgtcattgcc agatattgct ttaccggagc ctgaaacgcc gcctgccgca acggttgtcg
    51121 ctcaggaggg cgaagatgaa tatcagttga ttgttcgcgt gctgaaagaa actaacggcg
    51181 tggttgccgg gcctaaaggc gctgcgcaac gtctggggct gaaacgcacg accctgctgt
    51241 cacggatgaa gcgactggga attgataaat cggcattgat ttaactgcaa attgccggac
    51301 agatctgcct gtccggcata ctattcacga ggttttttcg gacgatattt ttccggcagt
    51361 tctggcaccg gacacttgtc atcgatgaga tgacgcacgg ttaagatcgg atgacgccac
    51421 agcattctcg gcccggccca acgcataatc tgtttcatct cttcacgctt tgcaggctgg
    51481 taacagtgca ccggacactg cttacaggct ggtttctctt cgccgaacac acatttatcc
    51541 agccgctttt gcgcgtaaac aaacaacgcc tcgtaatgct ccggctccgc tgacgcctgc
    51601 gggcatttcg cttgataaag atcgatcatt tttttaatcg tcagtttttc acgagagata
    51661 cgcttgccgg acatactgcc tccacctcat taagatgcat ttatattaca acttaatctt
    51721 aaagggcact atgactctaa agaagaaggg ttagccaacc gatacaattt tgcgtacttg
    51781 cttcataagc atcacgcaaa agctgcaaaa cagcatcttt cccggaacca gcatcaagaa
    51841 ctcgccgttc gcttcttccc ctgaaatgat taactccggt atcatgtgcg ccttatgtga
    51901 ttacaacgaa aataaaaacc atcacacctc atttaatatc agggaaccgg acataacccc
    51961 atgagtgcaa tagaaaattt cgacgcccat acgcccatga tgcagcagta tctcaagctg
    52021 aaagcccagc atcccgagat cctgctgttt taccggatgg gtgattttta tgaactgttt
    52081 tatgacgacg caaaacgcgc gtcgcaactg ctggatattt cactgaccaa acgcggtgct
    52141 tcggcgggag agccgatccc gatggcgggg attccctacc atgcggtgga aaactacctc
    52201 gccaaactgg tgaatcaggg cgagtccgtt gccatctgcg aacaaattgg cgatccggcg
    52261 accagcaaag gtccggttga gcgcaaagtt gtgcgtatcg ttacgccagg caccatcagc
    52321 gatgaagccc tgttgcagga gcgtcaggac aacctgctgg cggctatctg gcaggacagc
    52381 aaaggtttcg gctacgcgac gctggatatc agttccggtc gttttcgcct gagcgaaccg
    52441 gctgaccggg aaacgatggc ggcagaactg caacgcacta atcctgcgga actgctgtat
    52501 gcagaagatt ttgctgaaat gtcgttaatt gaaggccgtc gcggcctgcg ccgtcgcccg
    52561 ctgtgggagt ttgaaatcga caccgcgcgc cagcagttga atctgcaatt tgggacccgc
    52621 gatctggtcg gttttggcgt cgagaacgcg ccgcgcggac tttgtgctgc cggttgtctg
    52681 ttgcagtatg cgaaagatac ccaacgtacg actctgccgc atattcgttc catcaccatg
    52741 gaacgtgagc aggacagcat cattatggat gccgcgacgc gtcgtaatct ggaaatcacc
    52801 cagaacctgg cgggtggtgc ggaaaatacg ctggcttctg tgctcgactg caccgtcacg
    52861 ccgatgggca gccgtatgct gaaacgctgg ctgcatatgc cagtgcgcga tacccgcgtg
    52921 ttgcttgagc gccagcaaac tattggcgca ttgcaggatt tcaccgccga gttgcagccg
    52981 gtactacgtc aggtcggcga cctggaacgt attctggcgc gtctggcgtt gcgtaccgct
    53041 cgcccacgcg atctggcccg tatgcgtcac gctttccagc aactgccgga gctgcgtgcg
    53101 cagttagaaa ctgttgatag tgcaccagta caggcgctac gtgagaagat gggcgagttt
    53161 gccgagctgc gcgatctgct ggagcgagca atcatcgaca caccgccggt gctggtacgc
    53221 gacggtggtg ttatcgcatc aggctataac gaagagctgg atgagtggcg cgcgctggct
    53281 gacggcgcga ccgattatct ggagcgtctg gaagtccgcg agcgtgaacg taccggcctg
    53341 gacacgctaa aagttggctt taatgcggtg cacggctact acattcaaat cagccgtggg
    53401 caaagccatc tggcacctat caactatatg cgtcgccaga cgctgaaaaa cgccgagcgc
    53461 tacatcattc cagagctaaa agagtacgaa gataaagtcc tcacttcaaa aggcaaagca
    53521 ctggctctgg aaaaacagct ttatgaagag ctgttcgacc tgctgttgcc gcatctggaa
    53581 gcgttgcaac agagcgcgag cgcgctggcg gaactcgacg tgctggtgaa cctggcggaa
    53641 cgggcctata ccctgaacta cacctgcccg accttcattg ataaaccggg cattcgcatt
    53701 accgaaggcc gccatccggt ggttgaacag gtgctgaacg agccatttat cgccaacccg
    53761 ctgaatctgt caccgcagcg ccggatgttg attattaccg gtccgaacat gggcggtaaa
    53821 agtacctata tgcgccagac cgcactgatt gcgctgatgg cctatatcgg cagctacgta
    53881 ccggcgcaaa aagtcgagat tggcccgatt gaccgtatct ttacccgcgt aggggcagcg
    53941 gatgatctgg cttccgggcg ttcaaccttt atggtggaga tgaccgaaac cgctaatatt
    54001 ctgcataacg ccaccgagta cagtctggtg ctgatggatg agattgggcg cggaacgtcc
    54061 acttacgatg gtctgtcgct ggcgtgggcg tgcgcggaaa atctggcgaa taagattaag
    54121 gcgttgacgc tgtttgccac ccactatttc gagctgaccc ggttaccgga gaaaatggaa
    54181 ggcgtcgcca acgtgcatct cgatgcactg gagcacggcg acaccattgc ctttatgcat
    54241 agcgtgcagg atggcgcggc gagcaaaagc tacggcctgg cggttgcagc tctggccggc
    54301 gtgccaaaag aggttattaa gcgcgcacgg caaaaactgc gtgagctgga aagcatttcg
    54361 ccgaacgccg ccgctacgca agtggatggt acgcaaatgt ctttgctgtc cgtaccggaa
    54421 gaaacctcgc ctgcagtcga ggcactggaa aacctcgatc cggattcact caccccgcgt
    54481 caggcgctgg aatggattta tcgcctgaag agtctggtgt aataataatt cccgatagtc
    54541 ttttgctatc gggaatatta acgataactg acgaatcaaa taaaaatacc ctgtataata
    54601 ggaaagctta ttttacaggg taaaaccatg ccatctacac gctatcaaaa aatcaatacc
    54661 catcactatc gccatatatg ggtcgttggt gatattcatg gtgaatatca gttattacaa
    54721 tcccgcttac atcaactctc ttttttcccc gaaaccgact tacttatttc tgtcggcgat
    54781 aatattgatc gtgggccgga gagtcttaac gtcctgcgcc tgctaaacca gccctggttt
    54841 atctccgtta aaggcaacca cgaagcaatg gcgctggatg cgttcgcgac cggcgatggc
    54901 aatatgtggc ttgccagcgg tggtgactgg tttttcgatt taaatgattc agagcaaaaa
    54961 gaagctacag atctgttgct gaaattccat caccttccac atattattga aatcactaac
    55021 gacaacataa aatatgtcat cgcacatgca gattatccgg ggagtgaata tctctttggt
    55081 aaagaaatag cggagagcga attactctgg cctgttgatc gtgtgcagaa atcgcttaat
    55141 ggcgagttac aacaaataaa cggcgctgat tattttatat ttggacatat gatgtttgat
    55201 aacattcaga cgttcgctaa ccagatttat attgataccg gatcgccgaa aagcgggcgg
    55261 ctgtcatttt ataaaataaa gtaatgaggc cgggtaaagc aaagccacta cccggcactt
    55321 tttattagcg cttaccttcc gccagcagcg gcgggatgct cggcaccatt ggcgcgtcat
    55381 caatatcttt ctgcgtcatg cggaacgctt cgggataatg ttcgcggctg gtacggcgca
    55441 gcggttcggt atcgcgccag gtataaaggc aatgctggca ctgatatacc gtccagacat
    55501 ctttcaccgg cgatttcgcc atcacttcaa tctgttcatc ggcacaacgt ggacaaatca
    55561 tcttgttctc cttatttacg tgcggccagc atagcggtca gtttttcagc ccaggctttg
    55621 gtttccggca aatccaccac cggctggctg tagtgaccac ggttgtccgg ggcgacaggc
    55681 gtggtggcgt cgataatcag cttgtcggtg atccccgccg ggcttgagcc agggtcgagt
    55741 tccagcacgg acatattcgg caactgcacc aaatcccctg ccggatttac tttcgaggag
    55801 agcgcccaca tcacctgcgg caggttgaac gggtcaacgt cttcatcgac cataatcacc
    55861 atctttacgt agcccagacc gtgcggcgtg gtcatcgcac gcaggcccac cgcgcgggca
    55921 aagccgccgt agcgtttttt ggtggagata atcgccagca ggccgtgggt gtacatggcg
    55981 tttaccgcct gcacttccgg gaactcggct ttcagttgtt gatacagcgg cacacaggtg
    56041 gctggcccca tcaggtagtc gatttcggtc cacggcatgc cgaggtacag cgattcgaaa
    56101 atcggcctgg tgcggtaaga gactttatcg atacgcacca cggtcatgtt acgcccgccg
    56161 gagtagtgcc cggtaaactc accgaacggc ccttcgattt cgcgtttgcg gctttcgata
    56221 accccttcga ggatcacttc tgaaccccac ggcacatcaa aaccagtcaa tggcgcggtg
    56281 gcgatcgggt acgggctttc gcgcagcgcg cctgccattt catactcaga ctgatcgtat
    56341 ttcagcggcg tggcgcccat aagggtgatg atcggatcgt tacccagggt gatggcaatc
    56401 ggcagatctt caccgcgctc ttccgcttta tgcagatgca gggcgatatc gtgcatcggc
    56461 accggttgca ggccgagctt acgcttgccc ttcacttcca tgcggtagat accgacgttc
    56521 tgcttgccga agttatccgg gtcgagcgga tcgcgggaaa ccacgcacgc tttgtcgaga
    56581 tagaaaccgc catcaccgtc gtttaaacga aacagcggca ggatatcgaa cagattaatc
    56641 tcgtcaccat cgacggtgtt ctgcgcccag gctggattgg cgcggcgctc cggggcgatc
    56701 gggaagttat cccagcggcg gataaactca tcaatctgtt ttttaaccgg ggtgtttggc
    56761 ggcaggccca aggaaatcgc gtggttctgc caggaaccga tggtattcat cgccacgcgg
    56821 gcatcggtaa aaccgcgaat attatcaaac cacagcgccg gtgcaccatc gccgatacgc
    56881 ccggtggcgt tggcagcggc agccagatcc ggttccgcgt tcacctcttc gctgattttc
    56941 agtaactggc cgtggtcatc aagcgcctgt aaaaagctgc gtaaatcatc aaatgccatt
    57001 attcattctc ctgagaaaaa ttccgggcct gcggcaatcc ttgccagcgc ctggcgtagg
    57061 gatgttcaag gccaaattga tccagcacgc gggctaccac gtggtggaca atgtcatcta
    57121 ccgtttcggg atggttataa aaggcaggca tcggcggcac catcgccacg cccatgcgtg
    57181 aaagtgcgag catattttcg agatggatgg tgctaagcgg catttcacgc ggcaccagca
    57241 ccagtttgcg gccttcttta agcacgacgt ccgccgcgcg ccctaccagg ccatcagcgt
    57301 aaccagcgcg gataccggcg agcgttttca tactgcacgg aataacgatc atgccgtctg
    57361 tacgaaagga acctgaggag atggtcgccg cctgatccgc cgggttatgg ctgaagtcag
    57421 cgagggcagc aacatcgcgg gcgctgtaag gcgtttccag ttcaatggtg gttttcgccc
    57481 acttcgacat caccagatga gtctcgacat tcggcatctc ccgcagcgct tgcagtaatg
    57541 ccacaccaag aggcgcaccg gtagcccctg tcatcccgac gatcagtttc attgcttact
    57601 ctcttaattg ttcgtatacg aacattatta ttaaaatacg cctctgttct cattgcggca
    57661 agcactgaaa agtaacaaca tccccttcgt cactgaaaaa ccgctatgat agcggcagat
    57721 agtttgcccg gagtttacat gacgttacga aataaagcgt tccatcagtt acgacagctt
    57781 tttcagcagc acacggctcg ctggcagcac gagttacctg acctgaccaa accacagtat
    57841 gcggtgatgc gcgccattgc tgataagcct ggtatcgaac aggtggcact gatagaagca
    57901 gcagtcagca ccaaagcaac gttggcagaa atgttggcaa gaatggagaa tcgtggctta
    57961 gtcagacgtg aacatgatgc cgctgacaag cggcgtcgct ttgtctggct gactgccgaa
    58021 ggagaaaaga tacttgctgc ggcgataccg attggtgaca gcgtggatga ggaatttttg
    58081 gggcgtttga gtgcagaaga gcaggagctg tttgtgcagc tggtgcgcaa gatgatgaac
    58141 acataggatg caaattgccg ggtaggacgc tgacgtgtct tatccaggcg acaaaaacac
    58201 aagcttccca aacaaaagaa aaaggccagc ctcgcttgag actggccttt ctgacagatg
    58261 cttacttact cgcggaacag cgcttcgata ttcagcccct gcgtttgcag gatttcgcgc
    58321 aaacggcgca ggccttcaac ctgaatctgg cgaacacgtt cacgggtgag gccaatttca
    58381 cgacctacat cttccagtgt tgccgcttcg taccccagca aaccgaatcg acgtgccagt
    58441 acttcacgct gtttggcgtt cagctcgaac agccatttga cgatgctctg cttcatatcg
    58501 tcatcttgcg tggtatcttc cggaccgttc tctttttcat cggccaggat gtccagcaac
    58561 gctttttcgg aatcaccacc cagcggggtg tctaccgagg taatgcgctc gttaagacga
    58621 agcatacggc tgacgtcatc aactggctta tccagttgct ctgcgatctc ttccgcactt
    58681 ggttcatggt ccagcttatg ggacaactca cgtgcggttc gcaggtaaac gttcagctcc
    58741 tttacgatgt gaatcggcaa acgaatagta cgggtttggt tcataatcgc ccgttcaatc
    58801 gtctggcgaa tccaccaggt tgcgtatgtt gagaagcgga aaccacgttc cgggtcaaac
    58861 ttctctaccg cacggatcag ccccaggttg ccctcttcga taaggtccag caacgccaga
    58921 ccacgattgc cataacggcg ggcaattttt accaccagac gcaagttact ctcgatcatc
    58981 cggcggcgag aggcgacatc tccacgcagt gcgcgacgcg caaaataaac ttcttcttcg
    59041 gccgttaaca gtggtgaata accaatctca ccaaggtaaa gctgagtcgc gtccaacaca
    59101 cgctgtgtgg ctccctgcga taacagttcc tcttcggcca aatcgttatc actgggttcc
    59161 tcttctacta aggccttttc gtcaaaaacc tcaactccgt tctcatcaaa ttccgcatct
    59221 tcatttaaat catgaacttt cagcgtattc tgactcataa ggtggctcct acccgtgatc
    59281 ccttgacgga acattcaagc aaaagcctgg ttccgccgat ttatcgctgc ggcaaataac
    59341 gcagcgggtt tacggatttc cccttgtaac gaatttcaaa atgcaagcgt gttgaactgg
    59401 ttccggtgct acccatggtt gctatttttt gccccgcctt cacttcttgt tgttcccgga
    59461 ccagcattgt gtcgttatgg gcgtaggcac tcaggtaatc atcattatgt ttgatgataa
    59521 tcagattacc gtagccgcgc agcgcgttac cggcataaac aacacggcca tctgcggtcg
    59581 cgataattgc ctgtcctttg ctgcctgcga tatcaatccc cttgttgccc ccctcagaag
    59641 cgccaaaggt ttcgatcact ttgccctcag tcggccagcg ccaggtggag ataggcgtac
    59701 tggttgatgt actgctgaca gtcggctcgg ttgtgcttgc tgttggtacc gttacaggcg
    59761 ctgtgaccgt ggtcgcagtt ggcttgttgt tcggcaacat tttgttagca ctctgttcac
    59821 ccgaagactc agaatacgta attgtcggtt gcgacgcaac agcaacggtg gaattttgtg
    59881 caggcttgat cacaactcct tgctctgctg cgtcggcctg ggtaatggca tttccgccag
    59941 tgattggcgt accggaagca ttacccacct gtaaggtctg accgacgttc agcgcgtatg
    60001 gtgcctgaat attgttgcgc tgagcaaggt cacggaaatc gttgccagta atccaggcga
    60061 tatagaaaag cgtgtcgcct tttttcacgg tataggtact gccgctataa ctgcctttcg
    60121 gaatgttccc atactgacgg ttatagacga tgcgtccgtt ttccatctgt accggctgct
    60181 gagctactgg ctgcactggc tggatttgcg gttgttgagt agcctgaatt tgtggctgct
    60241 gcaccggctg aatttgcggt tgctgcgctg tagacgtcgt ccccattttc ggcggcggcg
    60301 taatcaacat accagaattg gtatgtgcag gcgcattgcc attaacggag ctgaccggtg
    60361 ccggtggatt tgaagtgtca gaacagcctg ccagccatag cgaaaccagt gacaaagccg
    60421 caatgcggcg aacggtgaat tttgggcttc ccgcgctcat ttatccccca ggaaaaattg
    60481 gttaataacc agtgacataa ttaccgtgca aggcaccata ctgaacactg gaaaagatgt
    60541 tcacgatacg ctgacctgcg gcaaaataac caggaaaaat ccaggtattt cctcacgttt
    60601 taagccagct cacccttcac taaagggaca aagcgcacgg cctccacggt atcgataata
    60661 aattcgcctc cccgacgacg cacccgtttc aaatactggt gctcctcccc tacgggtaag
    60721 acgagaatcc cgccttcgtc cagctgcgtc attagcgcag ttggaatttc cggcggtgcc
    60781 gccgtaacaa tgatagcgtc aaacggcgca cgtgcctgcc aaccttgcca tccatcgcca
    60841 tgacgggttg aaacattatg taaatcaaga tttttcaggc ggcgacgcgc ctgccactgc
    60901 aagcctttaa tccgttcaac cgagcaaaca tgctggacaa gatgcgccag gattgccgtt
    60961 tgatatcccg aaccggtgcc aatttccagc acccgcgact gcggcgtcag ctcgagtaat
    61021 tcggtcattc gcgccaccat atacggctgc gaaatcgtct gcccctgacc tatcggcaag
    61081 gcgatattgt cccaggcttt ttgttcaaac gcttcatcaa cgaatttttc acgcggcacg
    61141 gcggcaagtg cattcagcac ctgctcatcc tgaatacctt gcgcacgtaa ttgatccaga
    61201 agtgcttgta cgcgtctgct taccattgcg tgccaactcc cacgctgttt aaccagtctg
    61261 aaaccacatc ttgcgcgcta tgcgcagtta aatccacatg cagcggcgtg atggagacat
    61321 agccctcatc taccgcagca aaatcggtcc ccggaccggc atcacattta ccgcccggcg
    61381 ggccaatcca gtacagcgta ttgccgcgcg gatcttgctg cgggatcacc tgatctgccg
    61441 gatgtcgtgt accgcagcgc gtcacgcgaa tacctttgat ttgatccaag ggtaaatccg
    61501 gaacattaat attaagaata cgcccggtgc gcagcggctc tttacacagt gcgcgcaaaa
    61561 ttgaacaggt taccgccgcg gcagtgtcgt aatgtttatg cccgtcaagc gagacggcaa
    61621 gcgccggaaa acctaaatga cggccttcca tcgcggcggc taccgtaccg gaataaataa
    61681 catcatcccc cagattcggc ccggcgttaa ttccggacac aacaatgtcc gggcgcggac
    61741 gcatcagagc attcacgcca agatagacgc aatcggtcgg ggttcccatt tgcacagcaa
    61801 tatcaccatt ttcaaaggta aacgtgcgca gggaggattc cagtgtcaga gaatttgaag
    61861 cgccgctgcg gttacgatcg ggggcgacca cctgaacgtc agcaaactca cgcaaggctt
    61921 tcgccagcgt ttgtataccg ggtgcatgta ccccgtcatc attactcagc aatatgcgca
    61981 taatcacctg ttgtgttgat aagttccctg acaacgctgg ttgcaaaact acccgccgga
    62041 agccagaaac ggatttctac ggtgacgtca tcccaccaat tccagcttaa ttgttgcgga
    62101 tacagcagca tcgctctgcg cgcggcttca actttttcgc gcaccagtaa agcttgtaat
    62161 tcagtttctg cggcgacagc tgcctgttcg aatgccagcg cttcacgctg agttccccat
    62221 tcaccactgc ccggcaatgc ggcggttatc atcagctctt tatcgttgac gcgacgctgt
    62281 aattccacca gttcttcggt ggttgcaaca aaccagctac cacgtccggc taattgtagc
    62341 gcatcgccgt caacaacttg attaacgtct gcttttttga ggcgctcagc aacaatctga
    62401 ttaaacaacg cactgcgggc tgccgacaac caaaaactcc gtttattgcg atcgcgcacc
    62461 ggagtattgg tttgcgccca gcgcagcgcg ccctgcaagt tgctaccgcc aatcccaaaa
    62521 cgttgggcac cgaagtagtt cggtacacct tttacgcaaa tatcgatcag acgttgttca
    62581 acgtcatcgc gattgctcac ttcgcgcaga accagggtaa aggcgttacc tttcagcgcc
    62641 cctaaacgca gcttgcgctt gtgccgcgca tactccagca cctggcagcc ttccaattga
    62701 aaggcgctca gatcgggcat ttccttgccc ggcacgcgag cgcataacca ctgttctgta
    62761 acagcatgtt tgtctttttg cccagcaaag ctgacttcac gggcatgaat tttcaggaat
    62821 ttcgccagtg catccgccac aaaacgggta ttgcagccgt ttttgaggat tctaaccaga
    62881 atatgctcac cttcaccatc aggctcaaag cccaaatctt ccaccaccac aaagtcttcc
    62941 ggattggctt tcagcagccc ggtgccttgc ggtttaccgt ggaggtaagt gagattatca
    63001 aactcaatca ttttgttgcc ttaatgagta gcgccaccgc ttcacaggca atcccttccc
    63061 cacgtccggt aaatccaagt ttttccgtag tagtggcttt cacgttaaca tcatccatat
    63121 ggcagccgag atcttcggca ataaacacgc gcatttgtgg aatgtgcggc aacatcttcg
    63181 gtgcctgagc gatgatagtg acatcgacgt tgccaagggt ataacccttc gcctgaatac
    63241 gacgccaggc ttcgcgtagc agctcgcggc tatcagcacc tttaaatgcc ggatcggtat
    63301 ccgggaacag tttgccgata tcccccagcg ccgccgcgcc aagcaatgca tcggtcaacg
    63361 catggagcgc cacgtcgcca tcagaatgcg ccagcaatcc tttttcgtaa ggaatgcgta
    63421 cgccaccaat gataattggg ccttcaccgc caaaggcgtg tacgtcaaaa ccgtgtccaa
    63481 ttcgcattat gtattctcct gatggatggt tcgggtgagg taaaactcgg ccagtgccaa
    63541 atcctccggg cgcgtgactt taatgttatc cgcacggcct tcgaccaact gaggatggaa
    63601 tccgcaatat tccagcgccg aggcttcgtc ggtaatagcc gcgccttcat ttagagcgcg
    63661 cgtcagacag tcatgtaaca gctcacgagg gaaaaattgc ggcgtcagcg cgtgccataa
    63721 gccgttgcga tcaacggtat gagcaatggc atttttgccc ggttcggcac gtttcatagt
    63781 atcgcgcact ggtgcggcta ggatccctcc cgtgcggctg gtttcgctca acgccaacaa
    63841 tcgcgcgagg tcatcctgat gcagacaagg acgagcggcg tcatgcacca atacccactg
    63901 cgcgtcgcca gcggctttca aacctgccag cacggaatcg gcacgctcat caccgccatc
    63961 tacaacggtg atttgcggat gattcgccag aggaagttgt gcaaaacggc tatcgccagg
    64021 acttatggca atgacgacac gtttcacccg gggatgcgcc agcagcgcat acaccgagtg
    64081 ttcaagaatg gtttgattac cgattgagag atattgctta ggacattccg tttgcattcg
    64141 acggccaaat ccggccgccg gaaccacggc gcaaacatcc aaatgagtgg ttgccatgtt
    64201 aattcccggg ctgatttatc gattgttttg ccccgcagac tgtgcgcgct tcgacgcgtc
    64261 aggcaccaga cgataaaaag tttcgcccgg cctggtcatg ctgagttcat tacgcgcacg
    64321 ctcttcgagc gcctcctggc cgccattgag atcgtcaatt tcggcaaaaa gttgatcgtt
    64381 tcgcgcttta agtttcgcgt ttgtagcttg ctgtgccgcc acatcatcat tgacgcgggt
    64441 atagtcatgt ataccgttct taccgaacca cagcgaatac tgtagccaga ccagaatagc
    64501 cagcaacagc agcgttagtt tacccatcct gccccctgaa aaacggcatc atcatcccat
    64561 gcatccgaag acgactctac atcctctgtt ggggataccg cgacaacgcg ggcaaatgta
    64621 ccacatttgt ccattgttac gtatacccag ggcgtgcaga acataatctc attattagtt
    64681 acggtttgaa ttatgaacag aggagacaag aaagtacaaa ttagcccagt agccacataa
    64741 acagtgcgcc aaacataatg cctactgtca tcagggtgaa aacaatactg tagcgtagct
    64801 ttccgtccat caatgaatgc agcgcaatcc ccaccactac cgcaacgggc atcagcgcca
    64861 gaaagaaagg ccaggtgtag ataaagaaga acagcgtgtt agagccataa atcaacatcg
    64921 gcatcgccag cgcaaataac caggagataa aaccgaccac ggcaccaggc agtgaccatg
    64981 tggtttcttc atcctcagta aggctgtcgt tatttgttag tgtaatgtta tggctattac
    65041 gcatatttga tcctgttact ttgacgaacc gggcatggaa acccggtggt gtctcaggat
    65101 ctgataatat cgttctgtct caacagatct aataattgct gtaccaaatt tgttactaat
    65161 tgttcaccat tgagatgaat ttctgccgat tcaggcgctt cgtaaacgga atctattccc
    65221 gtaaagttgc gcagttcacc ggcacgcgct ttcttatata agccttttgg atcgcgggct
    65281 tcgcaaatcg ccagcggcgt atcgacaaac acttcgataa agcgcccttc tcctacgcgt
    65341 tcgcgaacca tctggcgttc ggcgcggtgt ggcgagataa atgcggtcag caccaccagt
    65401 ccggcttcaa ccatcaaatt cgccacttca ccgacgcgac ggatattctc tttacgatcg
    65461 gcatcgctaa aaccgagatc gctgcataat ccgtggcgaa cattgtcgcc atccagcaga
    65521 tacgtactga cgccgagttt atgtaacgcc tcctccagcg ccccggcgac cgttgattta
    65581 ccggacccgg agaggccggt aaaccacagc actacaccac gatgaccgtg gtgtagctcg
    65641 cgttgttgca cagtgaccgg atggctatgc cagacgacgt tttcgtcatg cagcgccatt
    65701 atttctcccc cagcaaatcg cgcgcgcccc agtgtggaaa gtggcggcga accagggcat
    65761 tcaattccag ttcgaatgca ctaaattcag atggcgcagc agttgcctgg ctaactggct
    65821 catgcaccat accggcacct acggtcacat tgctcaggcg atcgataaaa atcagcccac
    65881 cggtaaccgg gttttgctga taacgatcta acaccagtgg ctcgtcaaaa gtgagatcca
    65941 ccaggccgat gccgttcagc ggcaggtttt caacttcgcg ttgggtaagg ttattaatat
    66001 caacctgata acgaatgcca tcaacacgag aacgcgtctt cttaccggca attttgatgt
    66061 cgtaactctg gcccggggaa agcggctgtt ccgccatcca taccacatcc accgacgcgc
    66121 tctgcacagc tggtaacgct tcgtctgccg ccagcagcag atcgccacgg ctgatgtcga
    66181 tctcatccgt cagcaccagg gtgatagctt ctccggcaaa ggcttcttcg cgatcaccat
    66241 caaaagtcac gatccgcgcg acgtttgatt ccacaccaga gggcagcact tttacacgtt
    66301 gcccgacttc cacgcgaccg gatgccagcg ttccggcgta gccacgaaaa tcgagatttg
    66361 ggcggttaac gtactgcacc gggaagcgca ttggctgggc atccaccact cgctgaatct
    66421 ccacggtttc cagcacttcg agcagtgtcg gaccgctgta ccacggcata ctttcacttt
    66481 gcgaagccac gttgtcacct tccagtgcgg agagcggcac aaagcggata tccagattac
    66541 ccggcagctg cccggcaaag gtcagataat cttcacgaat acgggtgaac gtctcttcac
    66601 tgtaatccac cagatccatt ttgttgatcg ccacgaccag atgtttgatc cccaacagtg
    66661 tggagataaa actgtgacga cgggtttgat cgagcacgcc tttacgggca tcgatcagta
    66721 agatcgccag ttcacatgtc gatgcgccag tcgccatatt gcgggtgtac tgctcgtgcc
    66781 ctggagtgtc ggcgataata aatttacgct tctcggtaga gaaatagcgg taggccacgt
    66841 caatggtgat gccctgttca cgctcagctt gcaggccgtc caccagcaga gccagatcca
    66901 gcttttcgcc ctgggtgccg tgacgcttac tgtcattatg cagcgatgag agctgatctt
    66961 catagatttg gcgggtatcg tgcagcagac gaccaatcag ggtacttttg ccgtcatcga
    67021 cgctaccaca ggtcagaaaa cgcagcaggc ttttatgttg ttgcgcaatc atccaggctt
    67081 cgacgccgcc ttcattggcg atttgttgtg caagtgcggt gttcatctta aaaatacccc
    67141 tgacgttttt tcagctccat agaaccagct tggtcgcggt caatcacgcg cccctgacgt
    67201 tcactggtgg tggaaaccag catctcttcg atgatctccg gcagcgtttg tgcatttgac
    67261 tccaccgcac cggtcagcgg ccagcagccc agcgtacgga aacgcaccat ccgtttttta
    67321 atcacttcgc ccggttgcag gtcgatacgg ttgtcatcaa tcatcatcaa cataccgtcg
    67381 cgttccagaa ccggacgttc cgcagcgaga tacagcggca caatgtcgat attttccagc
    67441 cagatgtatt gccagatatc ctgctcggtc cagttagaga gcgggaaaac gcggatgctt
    67501 tcgcctttgt taatctgccc gttatagttg tgccacagct ccgggcgctg atttttcggg
    67561 tcccagcgat ggaagcgatc acggaaagag tagatacgct ctttagcacg ggatttctct
    67621 tcgtcgcggc gcgcaccacc gaaggcggca tcaaaaccgt atttattcag cgcctgcttc
    67681 aggccttcgg tcttcataat atcggtatgt ttcgcgctgc cgtgcacgaa tggattaatc
    67741 cccatcgcca cgccttccgg gttttgatgc accagcagct cgcagccgta ggctttcgcc
    67801 gtacgatcgc ggaactcata catctcacgg aatttccagc cggtatcgac atgcagcaac
    67861 gggaaaggca gcgtacctgg ataaaacgcc ttgcgcgcca gatgcagcat gacgctggaa
    67921 tctttaccga tagagtagag catcaccgga tttgagaatt ctgccgccac ctcgcgaata
    67981 atgtggatgc tttccgcctc cagttgccgc aggtgagtaa gtcgtatttg atccataacc
    68041 gttcctttgc aataccgcta ttttcttgcc atcagatgtt tcgactatag ggagcgtaag
    68101 agaacgaatg aaattaccaa ttagaatgag tagttcctta acggaataac gatttggcaa
    68161 agctaatatc aaaaagtgct taaggcaccg gatttcgggc gtttaggaag atttgaaatt
    68221 gttttagcgc agcggcagtt tcatactatg gcggtaaaaa aatttgcatg gtatttaagg
    68281 actcactatg ttttccgcat tgcgccaccg taccgctgcc ctggcgctcg gcgtatgctt
    68341 tattctcccc gtacacgcct cgtcacctaa acctggcgat tttgccaata cacaggcgcg
    68401 acatattgcc actttctttc cgggacgaat gaccggaaca cccgcagaaa tgttatctgc
    68461 cgattatatt cggcaacagt ttcagcaaat gggttaccgc agtgatattc gtacgtttaa
    68521 tagccgatat atttataccg cccgcgataa ccgcaaaaac tggcacaacg tgacgggaag
    68581 tacggtgatt gccgctcatg aaggcaaagc gccgcagcag atcattatta tggcgcatct
    68641 ggatacctat gccccgcaga gcgacgcaga tgcagatgcc aatctcggcg ggctgacgtt
    68701 acaaggaatg gatgataacg ccgcaggttt aggtgtcatg ctggaactgg cagaacgcct
    68761 gaaaaatacg cctaccgagt atggtattcg atttgtggcg accagtggag aagaggaagg
    68821 gaaattaggc gctgagaatt tactcaagcg gatgagtgac accgaaaaga aaaatacgct
    68881 gctggtgatt aatctcgata acttaattgt tggcgataaa ttgtatttca acagcggtgt
    68941 aaaaacccct gaagcagtaa ggaaattaac gcgcgacagg gcgctggcaa ttgcgcgtag
    69001 tcatggaatt gccgcaacga ccaatccggg tttgaataaa aattatccga aaggcactgg
    69061 atgttgtaat gacgcagaaa tattcgacaa agcgggcatt gctgtacttt cggtggaagc
    69121 gacaaactgg aatcttggga ataaagatgg ttatcagcaa cgcgcaaaaa cagccgcatt
    69181 ccctgcggga aatagctggc atgacgtaag actggataat cagcaacata ttgataaagc
    69241 acttcctgga agaatagaac gtcgctgccg tgacgttatg cggataatgc taccgctggt
    69301 gaaggagttg gcgaaggcgt cttgatgggt tggaaaatgg gagctgggtg ttctaccgca
    69361 ggggcgggga attctaagtg atatccatca tcgcatccag tgcgcccggt ttatccccgc
    69421 tgatgcgggg aacacagcgg cacgctggat tgaacaaatc cctgggccgg tttatccccg
    69481 ctggcgcggg gaacacaaac cgaaacacac gatcaatccg aatatgagcg gtttatcccc
    69541 gctggcgcgg ggaacacttt ggtgacagtt tttgtcactg ttttggtgac ggtttatccc
    69601 cgctggcgcg gggaacacac taagcataca tatctgtttt taaacaaatt tattccacat
    69661 caacaatcta ccaactaaat tcaaacattt ccttattttt aaagaacaca taacctattg
    69721 attatcaaca ggaagaaaag caaccaaacg taacccatcc aaatccaccg gaatacgtct
    69781 gttttctccc caggtctgaa attcaaaacc cgactcggta ttggtcgccc aggccatcac
    69841 cacatttccg caaccagcca gttgggtaat ttgctgccag atcatctccc gaatacgttt
    69901 tgatgtatca ccaacataca caccggcacg cacttccagt agccagattg cgagccgtcc
    69961 acgtaagcgc ggagggacat tttctgtaac aaccacgacc atgctcatcc gccgcgcccc
    70021 cggtgaccac tatcacccag cgtttcaggt tcagggatgg caggcggtaa catatccggc
    70081 gcgggttgtg gtggttcaat ttcacctgca gcaaggactt tctcaattaa cggtattaat
    70141 ttgcccgtta acttagtgct acggaaaata tcgcgacagg ctaatctgac ttctttatca
    70201 ggttctgcgg gttgcctcgc tgctatttca aatgcctttg gcacaaccga atcaaattta
    70261 atgatatcgg ctatgtcata aacaaatgaa agcggtttgc cactatgaat aaatccaata
    70321 gcgggcgcat atcccgcggc taatactgcc gcttcagaaa taccgtacag acatgatgtg
    70381 gcagcactga tgcagcgatt cacaacatcg cctttttccc agtctttagg atcgtatttg
    70441 cgaccattcc atttcacacc atattgtttc gccagtaatg cataggtctg gcgaacgcgg
    70501 gatccctcaa ttccccgtag ctgatccact gaacggcgag ctggcggtgg ctcacgaaaa
    70561 cgtaattcat acattttgcg caccaccttc aggcgtagat cttccgttaa agccagcttt
    70621 gcctggtaga gtaatttatc tgcccgcgcc cctccgggtt gtccggaaga gtaaacgcga
    70681 acgcccgctt caccgaccca gaccagcagt gttcccaccg tggcggccag atgcaccgcc
    70741 gcgtgggaaa ctctcgttcc cggttcgagc ataatgcagg cgaccgatcc caccggaatg
    70801 tgcgtgcgga tcccggtttt gtcgatcagc acgaaagcgc cgtccagtac gtcgatttga
    70861 ccgtactgga ggaagatcat agaggtgcga tcttttaacg ggatcggact cagtggtaca
    70921 aacgtcacac ctctgctccg ggtttgatca gcatcagacc acagccgaac gcgcggcttt
    70981 tgccataccc ctgactgaga cgttgcagaa ataacccggg atcggtgacc gtaagcatcc
    71041 ccgtataatc cacgctactg aactggatca gttgccggga gttttcccgc cgcagttgct
    71101 gttgtcggta ggcatcaacc gaggtatcca gcagtgtaaa accgcttctc tccccctgcg
    71161 ctgccagcca gtccagcgcc gcctgttgtt gatgcaacca gacatcactt ccttccgcct
    71221 gccccctcac ctgccgtttc gcctccatca gcagatcgtg gcgcttgccc gctttacaga
    71281 ttgttggatt cgcccgcagg ttgaaacaaa gttgttgtcc ggtacgcagc tcgggcacaa
    71341 atgaccggca ttcgatggtg aacgtttcac tttccgccgg gcgttcctgc gacagtacaa
    71401 aaaagcgaaa cgcgccctgg agttcttccc gacgataaag aaattgcctt tctttgccgc
    71461 cagggaagag atcccacagc cactgatgca tcacatattc cccgcgatcc accaaatgca
    71521 gcaactgcgc aggcgaaagc tggccggtat gtaaggttat tcttgagagg tacatggttc
    71581 ctccttgctg agccacggcc cctgattgat ggtgcgttcc ccaaacagcc actgctgacg
    71641 atttaaagga acatcccggc gacgtaatat tttgctcgcg accaggccgt cgtgttcccc
    71701 ttcccaccag cattcatcct gaagtttcgg gagtgagact ttcagttcgc ggaaactatc
    71761 ctgatactgt tggtatgcgt tacgtaagac atcagacgcg ttgccttcga gcagtaacgg
    71821 cgcaagtggt aacgccagcg gatgactttt tcgccccaga taaagcggaa aaaccggatg
    71881 acgtaaaccg tcctgcaact gttcaaggct gtaaggcgca tcgggggttg ttgccaccgc
    71941 caccatccac caggcatcgg tgtagtagtc gcgccgggag ataatcgcgc tcagaagatc
    72001 aggggcgctc aactcttcgc gacggctgaa ataacgcgct ttacgcacct cttttggcat
    72061 ctggaccgtg tgataatccc gtgcccagcg cgggttacgg ctggcgcaaa ccaccagtga
    72121 atagtggcgg ttaaacgcgt ttaatcgttc ggtatcatca cgccgaatcc ctaccccggc
    72181 agccagcagc cccagcaatg ctgagcgcga aggcagttca tgggtatgac gcacttcgcc
    72241 gggggcatcg acgccccagg atgccattgg cccatgaagc tgaaaaatca aatattggct
    72301 cattagccgc cccttacgcg cagataaagt ccagcacgtc cttcatgctt ccctgtttgt
    72361 tcatcacgtc aaagcttgcg cattcggtct tctgttcata gaccgtattc atattttcgc
    72421 gaagcgttgt aatacgctgc accgccacat ctaactgccg ggtgccatta atgggttcat
    72481 agaaagccgc cgccagagaa cgtggttgtt cggtgccttt ttctgccagc gcccaggagg
    72541 cgtaggcacg gctggcaaag ctgttctgtt tgccggttgg ggagacttta agtgcggctt
    72601 ccgtaaaggc gcgcaaggtc tgattagcta acgcttcgtc accgccgagg ttttcgacca
    72661 gcagatcttt atcgatgcag atataggtgt agaacagcgc agaaccgaat ccggtttcac
    72721 caagatgccc ggcaccggca tcttcagaag cctggcgcaa atcatcaacg gcggtgaaaa
    72781 aatcatcttc gacaatcgtt tcactgacac caaatgcatg cgcgacctgg caggcggctt
    72841 caacattaaa ttcgggttta ttcgccagca tacgaccaaa catagcgata tctactgcca
    72901 tgcgatcttt acgtaacaaa gcgagatctt cctcttttgg cgcgcgcttt tcttcggcca
    72961 gttgatgggc cagcgctttt acggcgtcaa attctgccgg gctgatatgg actaattgtt
    73021 cagtttcggc gttagtgagc ggatcttttg gttttttgtc gtttttagct ttcccaagat
    73081 aatccgcaat ttttgccgcc cattcgatgg cttttttctc ttcgatgcct ttctcaatca
    73141 ggatagttgc cgcctcacgc gcaatacgcc cactgcgaat accaatatgg cccgccagtg
    73201 cctgttcaaa aagtgcagaa gtgcgccacg cacgtttcag actttgcgag gaaacgcgca
    73261 gtcgcgttgc tccacccagg accacggttt tcggcgctcc ggtgtcatca cggttaaggt
    73321 tggctgcagg gtaagcggtt aacaaatgaa gctgaataaa tgtcgtcatg agatagtcct
    73381 ttattgattt tgttcgttat cgacgtcgcc agcctggtaa tattccagcg cccagcggat
    73441 acggataaat tcggttggcc gctgatgacg gcggtgatga ttgagcagat cgtcgctctc
    73501 ctggcaccag cggaagacac cctctgccag agagtcaaga ttaacggaac cgtttaataa
    73561 tctgactacg cggcgtaact ggcgcagtaa ttcatccggt gtttttactg ccgacaggcg
    73621 agtaaaacgc ccctttgaca ttaccgccgc cagctgcgca gcaaaaggca gccgttcatc
    73681 aatggcttta acattagcgc taagtgcggc tatcagcgcc agtgcggtaa tacgccattc
    73741 aggttcatcc tgccacttta tttgtctgtt cttcagaaac aggcgaaatc catccgtcag
    73801 acaaacatca ttaaccgtcg tactacgccg cagactggca cgttcgccgc gtttctcctg
    73861 caattcctca tgccatttgc gcagcgtggc tttgtgctcc tcttttacaa tactcattca
    73921 gcagcctcct gctttttctc cctggcggct ttagcacttt gcttctccgc cgatgttgta
    73981 aaatatttct tgcgcgcggt catgacgcgt tccaaatcaa cgggctcata aggattggtg
    74041 aatacacgct cgtcaaaatc ctgacgtgcg aataaccaaa tttccttttg ccatttgccg
    74101 agtaattcat ccgcatcctg accttcttca atttggcgca ctaacctcag gaagcgatgc
    74161 tgagttttgt tccagaagtc gatatccaca aaactgaaat caccccttgc accttttgga
    74221 tcggagaacc atgcttcttt caatgcactc cgtaacagac tcagaatccg tgaagccgtt
    74281 tgcgcagcca gccgcagctt cggtatctgg ccttcttttt tattgagcag cagcgggaaa
    74341 tggtgttcgt accaacagcg cgctttcatg ttgtcgaaat cataaccaaa tccccacagg
    74401 cccacttttg cctgtttcag actgctggca ttaaagagtt tcaccaccag cgcgggaagt
    74461 tccgtattgt tttctgactt acccgtttcg ataaggccta accagtcgcg ccagattaaa
    74521 ccgcccggtt gtggtttaac ggagtaaaac tcaccgccct ctttaagtgg tacacggtaa
    74581 ggcgttaagg gatgctgcca catggcataa ttcgcaccgt aatttttggt agtcatcaaa
    74641 ctcagaagcg cgtcactctg ctcaccgcaa atatcgcagt tgccgactgt cgtggtatta
    74701 aaatcaatac gaatacgccg cggcattccc cagtacgcct ggagtttatt gacctgatca
    74761 tcggttacca ccgcaccggc cagttcgctg gtacgcgtcg ggccaagcca ggggaaaacc
    74821 agatcgtcaa attttttggg tagcggtaag tcggcttcat cctgcggcat cacgttgagc
    74881 cgcagtttgc gccacaaggg ggcttgttga ttgccctgat actcctgcaa ttcaatcaga
    74941 gtcgtcatcg gcccaccgcc gcgtaaaccg gtgcgatagc ctttgccacc tgacggcgca
    75001 tttaactgta gggagaacag agctaacgca gaacaatgag agcatacgtg ttcagtcacg
    75061 ccacgcttaa taaagtggtc tttattaaac ttcgttgttt gagcgccggg aatctcaggc
    75121 agtagcgaag cgacctgaac tttatcgccc atgagcacct cgaaatcctg cataaatgaa
    75181 ggtgaatctg ggccaaactg gaaagggtgt tctaatgaca gcaatgcttc ccgtagcttt
    75241 tcagcttcca gcccgtcttc ccagatatca tcccaacgac gataatcttt tggcgcgaaa
    75301 ctgctttgta gtaaccccag caaaaactgc catgccgccc cctggagatc tgcccgcggc
    75361 gcagcgatat cgacaacatt ttcatccgcc agatcgactg gcgccagctt gcctgttgtt
    75421 ccgtctttaa aacgaacggg caaccacggg gttgtcagaa gtgaaaacga gttcatatgt
    75481 atcctctcgt aaaaaagacc atccctggtc ggttgtccat cattccatcc ttttcggtgt
    75541 atcaactatt cagagagtcc tgtcgcaggt atctcaatca accttgccaa tcaatccctc
    75601 cctggccgaa tacccgcaac tttcgtcatc agtgactaaa atcacgtttg ccatttccgg
    75661 atcttgccgc tgttcaatgc accactgcct gaacgcttcc ccttccagta aagaaaactc
    75721 atcccgatgt tttttccacc agcttcgacg cactctgaca acgctcattt cccatgcgtg
    75781 agcaccggtg gcataaggct tcaccacacc ggcaatacag gtagccagcc acagggaaac
    75841 agattcctca gccagacgtg tcgacagctt ttccggaagg taatcgttga tattggcggc
    75901 atagccaggc ttgaagttca ggacaaactt tttagccatt gcgcgatcgc agtaatattt
    75961 gcccacttgc tcctgctcgc tgcgggcaaa tccttccggc attaccacgt cctcaccgta
    76021 gactgattca ataagaaggc gggctgcgtg tggcatttga atagcgcctt gctcacgcag
    76081 tacacgctgc gtcagccaga ttcgtccatg atcgggatag acatatgcac tgttacgcat
    76141 ggcactgccg aaccattcgt caccaggagc gtcgtcccag acgggggcca gaatcagcaa
    76201 ttcaggaggg gaacgctcgt cttttccgtc acgctttaac tgaccattaa tatcgcggat
    76261 atgccgctgt aatcgccccg ctcgctgaat cagcaaatca acaggggcca ggtcggagat
    76321 catttcgtcc aggtcacaat caacgctctg ctctaagacc tgagtacaaa tgaggacttt
    76381 tccggcacgc tgtgaaccgt cttctttacc aaagcgtgcc agcgtctcca tttcaattcg
    76441 ctggcgatcg ctaaaagcaa agcggctatg aaagagtgaa aggctggaag cgggaatgac
    76501 gccgcgggca agcagctgac gatgaacctt aatagcgtca tcgacagaat tccggatcca
    76561 ggcgatgcat tttccctgac ttaccgccga ttcgatacgc gcaatactct cttgttcact
    76621 atgaagccaa cccacgctga cgctacgctc aacgtctttg cgcgtcgcta cccggtgtga
    76681 gttcacatcg gatttcgtga catgcgtcag ccaggggtaa tcatcctttt caaggaacgg
    76741 agcttcttgc tggccctctg tgccacgcgc aaaggcggcg acgagtttgt cgcgctgctg
    76801 ttgagataac gtagcagaaa gcaaaatgac gctgtttccg ccacgcgcct gccgctcgat
    76861 cagcccttca agaatgcacg acatgtaagc atcacaggca tggatctcat cagccagcag
    76921 gattttgtta ctcaacccca gaagccgcag attattatgt ttaaacggca tcactgccat
    76981 catcgcctga tccagcgtgc cgacgccaat ttcagccagt agcgccttct tgttactgtt
    77041 ggcaaaccag gccgcacatc cctgactgaa tgtttgttca tccggttctt ctgacccgac
    77101 taaatcaccg gaccagagtg attcattgaa gcggtccatt aatgtgcggg cactgtgtgc
    77161 cagcaccaag ctggggcggg actctggcga atagaaagca agccaggttt tgaccagccg
    77221 atcgtacatg gcattggccg ttgccattgt tggcaggcca aaaaacaaac cctgtgcttt
    77281 cctcgcagcc atcaacctgt gcgccaggat aagcgccgct tctgttttac ctgcgccagt
    77341 cacgtcttcc agaataaata actgtggccc tggctggctg atatccagat ccagtacctt
    77401 ttgctgtaat ggtgtcgggt gctcaataaa aggaaacagc gtattaattc cggtgaaagg
    77461 tgcggtttct gcttttggag gaaagacggt taaggcgttt tgagcctgaa ctaaagtttt
    77521 ctgccagtaa tctttaatat ccattgggtg tgcgacgcgt ggaaaaaatc gcgttgacga
    77581 acccgtccag tctgcgagta cgactgttgc agagatatac caggaaagtt gttttaaaag
    77641 ttcaacgccc tcgtcatcat cccagaatgt gggaatctct atgagcggaa acagtgcctt
    77701 gatttcaagg agaaaatctc gcgcggcagc tttgtcttca ggcagaaaat tatccagctc
    77761 atcaatacgg tcaggtggtc gaccatgatg cccggtagtt atggacatcc acatctctat
    77821 tacacgtgta agtttacgag aagagagtga agatgaagga agcaactcct cacattcact
    77881 taaataataa ttccacagcc agtaacccag cgttgaatga gagatctttt cgtaattctt
    77941 tctggaacct tccggaatct tgagttcagg ggccaggtaa agttgctgaa aagagcgggc
    78001 aaattttcca atatcgtgcc agcacagcaa ccaagcgaaa aattgagccg cctgttcctt
    78061 gtcagaaatc cctaattgac gaaagtaatc agccagcccg aagcaatttc ttttaaccat
    78121 taaatagccc attgcggcca catccagcga atgccagcaa agaaggtgat agccgtcgcc
    78181 accctctttc tcgccacgtc ggg
//
LOCUS       DAZKIP010000013        65424 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZKIP010000013 DAZKIP010000000
VERSION     DAZKIP010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.013"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            167..1864
                     /gene="ypdA"
                     /locus_tag="ACHOUK_001025"
     CDS             167..1864
                     /gene="ypdA"
                     /locus_tag="ACHOUK_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFP9896522.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            1879..2613
                     /gene="ypdB"
                     /locus_tag="ACHOUK_001026"
     CDS             1879..2613
                     /gene="ypdB"
                     /locus_tag="ACHOUK_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFP9896523.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            2626..3483
                     /gene="ypdC"
                     /locus_tag="ACHOUK_001027"
     CDS             2626..3483
                     /gene="ypdC"
                     /locus_tag="ACHOUK_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9896524.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRLVEGEMLFIPARAANLPVNNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(3486..5981)
                     /gene="ptsP"
                     /locus_tag="ACHOUK_001028"
     CDS             complement(3486..5981)
                     /gene="ptsP"
                     /locus_tag="ACHOUK_001028"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFP9896525.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIIRNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSEPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHGLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCCIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQAFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(6006..7043)
                     /gene="ypdE"
                     /locus_tag="ACHOUK_001029"
     CDS             complement(6006..7043)
                     /gene="ypdE"
                     /locus_tag="ACHOUK_001029"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFP9896526.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHSAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFNYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(7043..8128)
                     /gene="ypdF"
                     /locus_tag="ACHOUK_001030"
     CDS             complement(7043..8128)
                     /gene="ypdF"
                     /locus_tag="ACHOUK_001030"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFP9896527.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVLISR
                     ESAHILVDSRYYADVEARTQGYQLHLLDATHTLTTIARQIIADEQLQTLGFEGQQVSW
                     ETAHRWQSELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKTSFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDEAARRV
                     ITEAGFSHYFGHNTAHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(8143..9390)
                     /gene="fryC"
                     /locus_tag="ACHOUK_001031"
     CDS             complement(8143..9390)
                     /gene="fryC"
                     /locus_tag="ACHOUK_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFP9896528.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(9412..9738)
                     /gene="fryB"
                     /locus_tag="ACHOUK_001032"
     CDS             complement(9412..9738)
                     /gene="fryB"
                     /locus_tag="ACHOUK_001032"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFP9896529.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(9957..10922)
                     /gene="glk"
                     /locus_tag="ACHOUK_001033"
     CDS             complement(9957..10922)
                     /gene="glk"
                     /locus_tag="ACHOUK_001033"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFP9896530.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            11126..12382
                     /gene="yfeO"
                     /locus_tag="ACHOUK_001034"
     CDS             11126..12382
                     /gene="yfeO"
                     /locus_tag="ACHOUK_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFP9896531.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            12497..12823
                     /gene="ypeC"
                     /locus_tag="ACHOUK_001035"
     CDS             12497..12823
                     /gene="ypeC"
                     /locus_tag="ACHOUK_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFP9896532.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(12963..14201)
                     /gene="mntH"
                     /locus_tag="ACHOUK_001036"
     CDS             complement(12963..14201)
                     /gene="mntH"
                     /locus_tag="ACHOUK_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFP9896533.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            14537..15739
                     /gene="nupC"
                     /locus_tag="ACHOUK_001037"
     CDS             14537..15739
                     /gene="nupC"
                     /locus_tag="ACHOUK_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFP9896534.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ACEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WIGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(15789..17978)
                     /gene="pdeA"
                     /locus_tag="ACHOUK_001038"
     CDS             complement(15789..17978)
                     /gene="pdeA"
                     /locus_tag="ACHOUK_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFP9896535.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSIYRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEVLARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(18186..18261)
                     /locus_tag="ACHOUK_001039"
     tRNA            complement(18186..18261)
                     /locus_tag="ACHOUK_001039"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18226..18228),aa:Ala,seq:ggc)
     gene            complement(18301..18376)
                     /locus_tag="ACHOUK_001040"
     tRNA            complement(18301..18376)
                     /locus_tag="ACHOUK_001040"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18341..18343),aa:Ala,seq:ggc)
     gene            18597..18956
                     /gene="yfeC"
                     /locus_tag="ACHOUK_001041"
     CDS             18597..18956
                     /gene="yfeC"
                     /locus_tag="ACHOUK_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFP9896536.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            18958..19350
                     /gene="yfeD"
                     /locus_tag="ACHOUK_001042"
     CDS             18958..19350
                     /gene="yfeD"
                     /locus_tag="ACHOUK_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFP9896537.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLLEAEEPLAEYAPGIRTPAYRQIISAIDNMTH
                     SEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(19390..20598)
                     /locus_tag="ACHOUK_001043"
     CDS             complement(19390..20598)
                     /locus_tag="ACHOUK_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFP9896538.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKELERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYLNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCTANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            20606..21031
                     /gene="tnpA"
                     /locus_tag="ACHOUK_001044"
                     /pseudo
     CDS             20606..21031
                     /gene="tnpA"
                     /locus_tag="ACHOUK_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(21149..22564)
                     /gene="gltX"
                     /locus_tag="ACHOUK_001045"
     CDS             complement(21149..22564)
                     /gene="gltX"
                     /locus_tag="ACHOUK_001045"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFP9896539.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYFQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALAFIAERENQQ"
     gene            22823..22898
                     /locus_tag="ACHOUK_001046"
     tRNA            22823..22898
                     /locus_tag="ACHOUK_001046"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22856..22858,aa:Val,seq:tac)
     gene            22943..23018
                     /locus_tag="ACHOUK_001047"
     tRNA            22943..23018
                     /locus_tag="ACHOUK_001047"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22976..22978,aa:Val,seq:tac)
     gene            23065..23140
                     /locus_tag="ACHOUK_001048"
     tRNA            23065..23140
                     /locus_tag="ACHOUK_001048"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23098..23100,aa:Val,seq:tac)
     gene            23145..23220
                     /locus_tag="ACHOUK_001049"
     tRNA            23145..23220
                     /locus_tag="ACHOUK_001049"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23178..23180,aa:Lys,seq:ttt)
     gene            23340..23681
                     /locus_tag="ACHOUK_001050"
     CDS             23340..23681
                     /locus_tag="ACHOUK_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFP9896540.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQHI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VENTTHIGIYI"
     gene            complement(23672..24598)
                     /gene="yfeR"
                     /locus_tag="ACHOUK_001051"
     CDS             complement(23672..24598)
                     /gene="yfeR"
                     /locus_tag="ACHOUK_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9896541.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERISLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     VILSEPFFLLCHRDSALAAEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNTLMAGREGDPLYQI"
     gene            24688..25686
                     /gene="yfeH"
                     /locus_tag="ACHOUK_001052"
     CDS             24688..25686
                     /gene="yfeH"
                     /locus_tag="ACHOUK_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFP9896542.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(25683..25901)
                     /gene="ypeB"
                     /locus_tag="ACHOUK_001053"
     CDS             complement(25683..25901)
                     /gene="ypeB"
                     /locus_tag="ACHOUK_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFP9896543.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(25903..27918)
                     /gene="ligA"
                     /locus_tag="ACHOUK_001054"
     CDS             complement(25903..27918)
                     /gene="ligA"
                     /locus_tag="ACHOUK_001054"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFP9896544.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTYCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(27989..28987)
                     /gene="zipA"
                     /locus_tag="ACHOUK_001055"
     CDS             complement(27989..28987)
                     /gene="zipA"
                     /locus_tag="ACHOUK_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFP9896545.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSA
                     PQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLN
                     SIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            29217..29978
                     /gene="cysZ"
                     /locus_tag="ACHOUK_001056"
     CDS             29217..29978
                     /gene="cysZ"
                     /locus_tag="ACHOUK_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFP9896546.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            30163..31134
                     /gene="cysK"
                     /locus_tag="ACHOUK_001057"
     CDS             30163..31134
                     /gene="cysK"
                     /locus_tag="ACHOUK_001057"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFP9896547.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            31518..31775
                     /gene="ptsH"
                     /locus_tag="ACHOUK_001058"
     CDS             31518..31775
                     /gene="ptsH"
                     /locus_tag="ACHOUK_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFP9896548.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            31820..33547
                     /gene="ptsI"
                     /locus_tag="ACHOUK_001059"
     CDS             31820..33547
                     /gene="ptsI"
                     /locus_tag="ACHOUK_001059"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFP9896549.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            33588..34097
                     /gene="crr"
                     /locus_tag="ACHOUK_001060"
     CDS             33588..34097
                     /gene="crr"
                     /locus_tag="ACHOUK_001060"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFP9896550.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(34140..34991)
                     /gene="pdxK"
                     /locus_tag="ACHOUK_001061"
                     /gene_synonym="thiJ"
     CDS             complement(34140..34991)
                     /gene="pdxK"
                     /locus_tag="ACHOUK_001061"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFP9896551.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMLVVVVTADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            35096..35463
                     /gene="yfeK"
                     /locus_tag="ACHOUK_001062"
                     /pseudo
     CDS             35096..35463
                     /gene="yfeK"
                     /locus_tag="ACHOUK_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
     gene            complement(35466..36377)
                     /gene="cysM"
                     /locus_tag="ACHOUK_001063"
     CDS             complement(35466..36377)
                     /gene="cysM"
                     /locus_tag="ACHOUK_001063"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFP9896552.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPNNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMADRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITYFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(36511..37608)
                     /gene="cysA"
                     /locus_tag="ACHOUK_001064"
     CDS             complement(36511..37608)
                     /gene="cysA"
                     /locus_tag="ACHOUK_001064"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFP9896553.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIEPRDEELALAQSA"
     gene            complement(37598..38473)
                     /gene="cysW"
                     /locus_tag="ACHOUK_001065"
     CDS             complement(37598..38473)
                     /gene="cysW"
                     /locus_tag="ACHOUK_001065"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFP9896554.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEENHEH"
     gene            complement(38473..39306)
                     /gene="cysT"
                     /locus_tag="ACHOUK_001066"
     CDS             complement(38473..39306)
                     /gene="cysT"
                     /locus_tag="ACHOUK_001066"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFP9896555.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(39306..40322)
                     /gene="cysP"
                     /locus_tag="ACHOUK_001067"
     CDS             complement(39306..40322)
                     /gene="cysP"
                     /locus_tag="ACHOUK_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFP9896556.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(40480..41271)
                     /gene="ucpA"
                     /locus_tag="ACHOUK_001068"
     CDS             complement(40480..41271)
                     /gene="ucpA"
                     /locus_tag="ACHOUK_001068"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFP9896557.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(41400..42257)
                     /gene="murR"
                     /locus_tag="ACHOUK_001069"
     CDS             complement(41400..42257)
                     /gene="murR"
                     /locus_tag="ACHOUK_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFP9896558.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATAQHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGCDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            42421..43317
                     /gene="murQ"
                     /locus_tag="ACHOUK_001070"
     CDS             42421..43317
                     /gene="murQ"
                     /locus_tag="ACHOUK_001070"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFP9896559.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNIGLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPVVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            43321..44745
                     /gene="murP"
                     /locus_tag="ACHOUK_001071"
     CDS             43321..44745
                     /gene="murP"
                     /locus_tag="ACHOUK_001071"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFP9896560.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PDIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            44750..46054
                     /gene="pbp4b"
                     /locus_tag="ACHOUK_001072"
     CDS             44750..46054
                     /gene="pbp4b"
                     /locus_tag="ACHOUK_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFP9896561.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMAQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDLIAKYISGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     THWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(46112..47011)
                     /gene="yfeX"
                     /locus_tag="ACHOUK_001073"
     CDS             complement(46112..47011)
                     /gene="yfeX"
                     /locus_tag="ACHOUK_001073"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFP9896562.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNIWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(47107..47682)
                     /gene="yfeY"
                     /locus_tag="ACHOUK_001074"
     CDS             complement(47107..47682)
                     /gene="yfeY"
                     /locus_tag="ACHOUK_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFP9896563.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADAVDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(47743..48192)
                     /gene="yfeZ"
                     /locus_tag="ACHOUK_001075"
     CDS             complement(47743..48192)
                     /gene="yfeZ"
                     /locus_tag="ACHOUK_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFP9896564.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWRVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEVKE"
     gene            complement(48179..48604)
                     /gene="ypeA"
                     /locus_tag="ACHOUK_001076"
     CDS             complement(48179..48604)
                     /gene="ypeA"
                     /locus_tag="ACHOUK_001076"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFP9896565.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            48818..49687
                     /gene="amiA"
                     /locus_tag="ACHOUK_001077"
     CDS             48818..49687
                     /gene="amiA"
                     /locus_tag="ACHOUK_001077"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFP9896566.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            49691..50590
                     /gene="hemF"
                     /locus_tag="ACHOUK_001078"
     CDS             49691..50590
                     /gene="hemF"
                     /locus_tag="ACHOUK_001078"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFP9896567.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREGGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGKDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(50596..51648)
                     /gene="eutR"
                     /locus_tag="ACHOUK_001079"
     CDS             complement(50596..51648)
                     /gene="eutR"
                     /locus_tag="ACHOUK_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFP9896568.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(51694..52194)
                     /gene="eutK"
                     /locus_tag="ACHOUK_001080"
     CDS             complement(51694..52194)
                     /gene="eutK"
                     /locus_tag="ACHOUK_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFP9896569.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGVTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(52207..52866)
                     /gene="eutL"
                     /locus_tag="ACHOUK_001081"
     CDS             complement(52207..52866)
                     /gene="eutL"
                     /locus_tag="ACHOUK_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFP9896570.1"
                     /translation="MPALDLIRPSVTAMRVIASVNADFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAQDTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(52876..53763)
                     /gene="eutC"
                     /locus_tag="ACHOUK_001082"
     CDS             complement(52876..53763)
                     /gene="eutC"
                     /locus_tag="ACHOUK_001082"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFP9896571.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTTCAAPVTSESCA
                     LDLGSAEAKVWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTLALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(53784..55145)
                     /gene="eutB"
                     /locus_tag="ACHOUK_001083"
     CDS             complement(53784..55145)
                     /gene="eutB"
                     /locus_tag="ACHOUK_001083"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFP9896572.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(55157..56560)
                     /gene="eutA"
                     /locus_tag="ACHOUK_001084"
     CDS             complement(55157..56560)
                     /gene="eutA"
                     /locus_tag="ACHOUK_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFP9896573.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKSLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDVRSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALIQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(56557..57783)
                     /gene="eutH"
                     /locus_tag="ACHOUK_001085"
     CDS             complement(56557..57783)
                     /gene="eutH"
                     /locus_tag="ACHOUK_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFP9896574.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKDDATAAKTEAEAQS"
     gene            complement(57883..59070)
                     /gene="eutG"
                     /locus_tag="ACHOUK_001086"
     CDS             complement(57883..59070)
                     /gene="eutG"
                     /locus_tag="ACHOUK_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFP9896575.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQLVGLYA
                     AAQ"
     gene            complement(59060..59896)
                     /gene="eutJ"
                     /locus_tag="ACHOUK_001087"
     CDS             complement(59060..59896)
                     /gene="eutJ"
                     /locus_tag="ACHOUK_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFP9896576.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTFAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(59907..61310)
                     /gene="eutE"
                     /locus_tag="ACHOUK_001088"
     CDS             complement(59907..61310)
                     /gene="eutE"
                     /locus_tag="ACHOUK_001088"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFP9896577.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     IAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAGKIA
                     AAIGLKVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(61322..61609)
                     /gene="eutN"
                     /locus_tag="ACHOUK_001089"
     CDS             complement(61322..61609)
                     /gene="eutN"
                     /locus_tag="ACHOUK_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFP9896578.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPHGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(61716..62009)
                     /gene="eutM"
                     /locus_tag="ACHOUK_001090"
     CDS             complement(61716..62009)
                     /gene="eutM"
                     /locus_tag="ACHOUK_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFP9896579.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(62048..63064)
                     /gene="pta"
                     /locus_tag="ACHOUK_001091"
     CDS             complement(62048..63064)
                     /gene="pta"
                     /locus_tag="ACHOUK_001091"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFP9896580.1"
                     /translation="MIIERCCELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(63061..63792)
                     /gene="eutT"
                     /locus_tag="ACHOUK_001092"
     CDS             complement(63061..63792)
                     /gene="eutT"
                     /locus_tag="ACHOUK_001092"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFP9896581.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPLGCQAIVGLSDEDLHRLSHQPLRYLGH
                     DHLVPEASHGRDAALLNLLRTKVREAETVAAQVFITRSFEVLRPDILQALNRLSSTVY
                     VMMILSVTKQPLTVKQIQQRLGETQ"
     gene            complement(63789..64490)
                     /gene="eutQ"
                     /locus_tag="ACHOUK_001093"
     CDS             complement(63789..64490)
                     /gene="eutQ"
                     /locus_tag="ACHOUK_001093"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFP9896582.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDELIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(64465..64944)
                     /gene="eutP"
                     /locus_tag="ACHOUK_001094"
     CDS             complement(64465..64944)
                     /gene="eutP"
                     /locus_tag="ACHOUK_001094"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFP9896583.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(64957..65292)
                     /gene="eutS"
                     /locus_tag="ACHOUK_001095"
     CDS             complement(64957..65292)
                     /gene="eutS"
                     /locus_tag="ACHOUK_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFP9896584.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
BASE COUNT        15403 a        18036 c        16572 g        15413 t
ORIGIN      
        1 caataaattt actaaccaga agtgcattat caagagatgc attgctaata caccatttat
       61 ctatcaatcc ccttttaaaa ttgtggtttt tcctcaaaaa cggcctgctg gtcatcagcc
      121 ttcaggttac ctatcataga ggtttaatcc ttattcagag tcacccgtgc acgaaatatt
      181 caacatgctg ctggcggtct tcgatcgggc agcgttaatg cttatctgcc tgttctttct
      241 catccgtatc cgcctgtttc gcgaactgtt gcacaagtcg gcacactcac cgaaagagtt
      301 gctcgccgtt accgccattt tctcgctgtt cgccctcttc agcacctggt ccggcgttcc
      361 cgtagaaggc tcgctggtga acgtacgtat tatcgcggtg atgtccggcg ggattctgtt
      421 tggcccgtgg gtgggcatca ttaccggcgt gattgcgggt attcatcgtt atttaattga
      481 tatcggtggc gtgacggcga tcccctgctt tatcaccagc attctggctg gttgtatatc
      541 gggctggatc aacctgaaaa tccccaaagc acagcgctgg cgcgtcggta ttctcggcgg
      601 catgttgtgt gaaacgttga ccatgattct ggtgattgtc tgggcaccaa ctaccgcgtt
      661 ggggatcgat atcgtctcta aaatcgggat tccaatgatc ctcggcagcg tctgtatcgg
      721 ctttattgtg cttctagtgc aaagcgttga gggcgaaaaa gaggccagcg ccgcgcggca
      781 ggccaagttg gcgctggata tcgccaacaa aacgctacct ctgtttcgcc atgtcaatag
      841 cgagtcatta cgcaaagttt gtgaaattat tcgcgatgac attcacgccg atgcggtggc
      901 gattaccaat accgatcatg tgctggccta tgttggtgtg ggtgaacata actatcagaa
      961 tggcgatgac ttcattagcc cgactacccg tcaggcgatg aattacggaa aaatcatcat
     1021 taaaaacaat gatgaagccc accgcacacc agagattcat tccatgctgg tgatcccatt
     1081 gtgggagaaa ggcgttgtga ccggaacgct gaaaatttac tactgccacg cgcatcagat
     1141 aacctcgtca ttacaggaaa tggcggttgg tctgtcgcaa atcatctcca cgcaactgga
     1201 ggtttcacgc gccgagcagc tacgtgaaat ggcaaataag gcagagcttc gcgccctgca
     1261 aagcaaaatt aatccccatt tcctgtttaa cgctttgaac gctatttcat cgtcgatccg
     1321 tctgaatccg gataccgctc gccagctgat ctttaatctg tcgcgttatc tgcgctataa
     1381 cattgaatta aaagacgatg agcaaatcga tatcaaaaaa gagctgtatc aaattaaaga
     1441 ctatattgcc attgagcagg cccgctttgg cgacaagctg acggttatct atgatattga
     1501 tgaagaggtg aattgctgca ttcccagcct gctgatccag ccgttggtgg agaatgccat
     1561 tgtccacggt atccagcctt gcaaaggtaa aggcgttgtc accatcagcg ttgcagagtg
     1621 cggcaatcgg gtacgcattg cggtgcggga taccgggcac ggcatcgatc caaaggtgat
     1681 tgagcgggtc gaagcgaatg aaatgccggg caataaaatt ggcctgctga atgtccatca
     1741 tcgcgtgaag ttattgtatg gcgaggggct gcatatccgc cgcctggaac caggaacgga
     1801 aattgcgttt tacattccta accaacgcac cccagtcgcc tcacaggcta cgttattgct
     1861 ttgagccgga gtgatattgt gaaagtcatc attgttgaag acgaattcct ggcacaacag
     1921 gaactgagct ggctaattaa agagcacagc cagatggaga ttgtcggcac ctttgacgac
     1981 ggtctggacg tgttgaagtt tttgcagcat aaccgcgtcg acgccatttt tctggatatc
     2041 aatattccgt cgctggatgg tgtgttgctg gcacaaaaca tcagccagtt cgcccataaa
     2101 ccgtttattg tgttcatcac cgcgtggaaa gaacatgcgg tagaagcgtt tgaactggag
     2161 gcgtttgact acattctaaa accgtatcag gagtcacgca ttaccgggat gctgcaaaaa
     2221 ctggaagcag cctggcaaca acagcagacc agcagcacgc ctgccgcgac ggtaacgcgt
     2281 gagaatgaca ccattaatct ggtgaaagat gagcgaataa tcgtcacgcc aattaacgat
     2341 atctattacg ccgaagcgca cgagaaaatg acctttgttt atacgcggcg tgaatcctac
     2401 gtaatgccga tgaacattac cgaattttgc agcaaactgc cgccgtcgca ttttttccgc
     2461 tgccatcgct cattttgtgt caatctgaac aaaatccgcg aaatcgaacc gtggtttaat
     2521 aacacctata ttctgcggct gaaagatctg gattttgaag tgccggtcag ccgcagcaaa
     2581 gttaaagaat tccgccagtt aatgcatctt taatcgggaa ctttcatgaa agcaccaggc
     2641 ttgcccgccg atcaacaatt tttcgccgat ctgttcagcg gcctggtgct taacccgcaa
     2701 ctactggggc gcgtctggtt tgccagccag cctgcctcgt tgccggtggg cagtttatgt
     2761 attgattttc cccgtctgga tatcgtgctg cgcggcgaat acggcaatct gctggaagca
     2821 aagcagcaac gtttggtgga aggagaaatg ctgtttattc cggcgcgcgc ggctaattta
     2881 ccggtcaaca acaaaccggt gatgctgtta agcctggtgt tcgctccgac ctggcttggg
     2941 ttatcgtttt acgatagccg caccacgtca ttgttgcatc ctgctcgcca gatccagctt
     3001 cccagcctgc aacgcggtga aggtgaagcg atgcttaccg ccctcaccca tcttagccgt
     3061 tcgccgctgg agcaaaatat cattcagccg ctggtgttaa gtttgctgca tctttgccgt
     3121 aacgtggtga atatgccgcc gggcaattcg cagccgcgcg gcgattttct ctatcacagc
     3181 atttgtaact gggttcagga taattatgcc cagccgctca cccgcgagag cgtggcgcag
     3241 ttttttaata tcacgcccaa tcatctgtca aaactgtttg ctcagcatgg aacgatgcgt
     3301 tttatcgagt atgtgcgttg ggtgcgaatg gcgaaggcga ggatgatttt gcagaaatat
     3361 catttgtcaa ttcatgaagt ggcacagcgt tgcggttttc cggatagcga ctatttttgt
     3421 cgcgttttcc ggcgtcagtt tggtctgacg ccgggagagt acagcgcccg ttttcagggc
     3481 taacgtcaga aggttaattc tgtttccagc agcgtcagga tactttgtgc atcttgcgcg
     3541 gcaaacaacg actggcggaa gtttttattc accagtttac gcgccagctg cgagaagact
     3601 ttcacatgat tcatcccttc gttagcaccc agcgtcagca tgatcaccag ttcgacttcg
     3661 cccatttctg actgccagtc aaccggtttc gccagccggg caatgctgat actggaatga
     3721 cggatccact gagatttggt gtgcgggatc gccacgccaa aaccaacgcc agtggtgaca
     3781 atctcttccc gctgccagac atcctcttcc agctcgaacg gatgttcagt gcgcccgtta
     3841 acgccgaggt taccgcacag gaactggatc gcctgctctt tattgctaaa ggcctgatca
     3901 acgaagatat tctccagcgc cagcagcggg cgaacgtctt cttccggcgt aaaggcggtg
     3961 agtaacgctt caatttcctg cgcgctgcga cattcacacg cctgacgtgc cagttcccga
     4021 cacgcctcgc tatccagttg acgaagctgg cttttcaccg ccggaatgcg cgggctactc
     4081 atactcagct catccaggcc cagcccaagc agtagcggca gataacggct ttcaccgccc
     4141 agttcaccgc aaatgcctac ccatttgccc cgctgatgcg cagtggtaac tatttgctgc
     4201 aacatgcgca ggaacgatgg cgtaatcggg ttatatagcg gcgatacgcg cgggttatta
     4261 cgatcgaccg catacagata ctgggtcata tcgttggagc cgatactgaa gaaatcgacc
     4321 tcatcgcaga agtgatcgat gatgcagcac accgacggca cttccaccat gatacccagc
     4381 gtaatcgtct ctgcatgacg caggccatcg cgcttaagct caacgatcgc tttttggatc
     4441 tcgcccttca cccataagat ctgatcgagg ccgtggacca tcgggatcat caactgggcg
     4501 ttgccgaaac tggcggcgcg caaaatggcc cgcagttgag tgcggaacag gccagcaaat
     4561 tccgggtaaa tacgtaccgc gcgatagccg aggaacgggt tctcttcctg gggaatattc
     4621 agataaggaa tgcttttatc gcctccgatg tccattgtgc ggaagataat cggcttgtcg
     4681 cccgctgcca gtagcacctg ctggtaggct tcaaattgct cctgctcgtc cggctcgctg
     4741 tcgcgatcca tatagagcat ttcggtacgg aacagcccga cgccttccgc gccgttggca
     4801 aacgcgccag gcgcttccag agcggtgcca atattcgccg cgatatcaat acgcttgtta
     4861 tcacgggaat aagccagctg cgcggcagcc tgcgcctgtt gtttttggcg tttatccgcc
     4921 agcgtctgcg cgacctgata ataaccgctc accgcgtcat tcgggttaat cgccagcacg
     4981 ccgcactggg cgtcaagcac tgcaggttgc ccggcataac gcgcgatcgc atccagcggc
     5041 aggccgctca gtaccgggat cgccgaggcg cgggccagaa tcagggtatg cgaggtgcgc
     5101 ccggttttct ccagaatcat gcccgcaaga ttttttaaat ccaggcttaa aaactggctt
     5161 ggggttaaat cctcggccac cagaatagtc ggtttttcca gcaccagatt gttgcgcggc
     5221 ttcagttccg gccaggtaat atgcagcaac tgttcgctaa tatcgcgaat gtcgctaaca
     5281 cgttcgcgca gataatcgct ggcagaagca gaaagtttgg cgcaaacctg ctccatattt
     5341 ctgatgatcg ccgcccccag cccctgatgc tgttctgtca tcaggcgacg gatattgcct
     5401 gcaaattcat catcctgaat cagcgacaaa tgggcgctga ggatagtttt gctttcgccg
     5461 tcacgctcac gcaattgctg gttcagttgc tcggcaagcg ttgccaggct gtgctccagc
     5521 cgggtggaat cttgcgcact ggcggggatt gcccgataac tgtcgaggct gtcgctttgt
     5581 aacagggtca gcgtacccac gccgacgccg cttgccagca cattgccgta cagtaaatcc
     5641 gggttaaggc ggcttaatga acgcggcagc ggatgcgccg tcagttctgc ctgcgtaggc
     5701 tgaacgctgt cgctgtcgat aaagcgcacc tggatgtact cttccagcac gcgccgcgcc
     5761 tgctcttcat cgctgccgct aatgttcagg ctgcaactgt cattaaatag ggtgccggtg
     5821 ccaatcagcg ccagcgagct tttggcatcg gcctttgcgt tctggcgatg gttaataaaa
     5881 gtgatttcgc tttgccactg gctgcactgt tctttaagtt cccacgccgg acgagcatgt
     5941 agaccgttag gcaaaggaca gagaaattga atcgttaaca taaccgctcc gggttaatca
     6001 ggagatcatc tgaaatccgt cagttgaaca accgtctcac gcgtaagacg ttgaataagg
     6061 gcagataaaa gttgctccat ctgcaaaatg tcacggcaat cggcaatcga cgcggcgcaa
     6121 tgtccgtggc gggtggcagg ccccatcacc actgtgggta cgccagtacc ggttaagtgc
     6181 accgctccac cgtccgtgcc gccgttactg aacatatccg cctgtaacgg cacgccaatt
     6241 tctgccgcca cggtttcgat ccaggcggtg agttttggcg gcgcaatcag tgacttgtcg
     6301 cttaacacca gcatcgggcc gttaccaatc tggcgatggt tagccgcgcc ataattaaag
     6361 tttttcgccc agcaggcggt atcaaggaca atggcgacgt ccggcgacac cgcgcgggtg
     6421 gcagtttgcc cgccgcgtaa ccccacctct tcgctggaac tggccaccag ccacacttcc
     6481 gcaggcagtt cagcgctgtg tagttcgcgc agtaacgtca ccagcaggta gcaaccgagg
     6541 cggtcatcaa aggctttccc catcacccgc tggtggggga gaacctgaaa agtggtatcg
     6601 aacgtgacgc gatcgcctgg acgaattccc gcctgcatca cttcgtcata cgagcgcgcg
     6661 ccaatatcca cgcgcatggc gctgacatca ttcccctgcc ggtcgccgtc aagcaggcct
     6721 ggaattttgc actcttcacg ggtggtgatg cgcaccggct gcagctggcg ggcagccatg
     6781 cgtacattgc caaccggcag cacatcaatc gccccttcgc gggagatgct gcgcaccata
     6841 aatcccactt cgtccatatg cgcacagatc atcaccttcg gacctgtcga ttcattgagg
     6901 cggatcagca ccgatcccag cccatcaaat cgcacttctt tttgcaggcg atccgcttct
     6961 tccagcagga tctgccgcac ttcctgttcc gaggaggcga tcgcatctgc ctcgctcaac
     7021 gctttcaata gcgataaatc cattatgcct ctcccgtgag caacactgtt ttcggcatgg
     7081 cgtagagcac ttctgcgcct tgcggggtga ccagcacaac atcctcgatg cgcacgcccc
     7141 cttgccctgg caaataaatc cccggctcca cggtcagtaa catgcctggc tgtagcgtcg
     7201 tggtgtcccg cggtgaaaaa cgcggatctt catgaacttc aatgccgata gcatgagcgg
     7261 tgttatgacc gaaatagtgg ctaaaacctg cctcggtaat cacccgacgc gcggcttcgt
     7321 caacctgctg gcagcgcacg ccggggcgaa ttgcagagat tgctgcgagc tgtgcctgca
     7381 aaacaatctg atagacgtta aacagcgggt gagattcggc gctcacccct tcgccattca
     7441 ccagcaaggt gcgcgtcata tcagagcagt agccctgata gagcgcaccg aaatcgagag
     7501 tgacaaactc gcccgctgca acaatcttgt cgctggcttt gccgtgcggc agcgccccac
     7561 gccagccact ggcgacaatg gtgtcaaaag aggttttttc tgcgccctgc tggcgcataa
     7621 accactccag ttcagcggct atctcgcgct cgctcatccc cgcctgaata aagcggcgaa
     7681 tatgctctgc accgcgatcg gcaatcccac aggcaaggcg gattttctcc acctcctctg
     7741 gcgttttgat ttgccgcagc acatccggcg tggcgctaac cagtttcgca ttgagttcag
     7801 actgccagcg atgcgcggtt tcccaactca cctgctggcc ttcaaaaccg agcgtttgca
     7861 actgctcatc ggcaatgatt tgcctggcga tggttgtaag cgtgtgcgtc gcgtcaagca
     7921 aatgcagctg gtaaccttgc gtgcgggctt ctacatccgc gtaatagcgc gagtccacca
     7981 gaatgtgcgc actttcacgg ctaatcagca catagcctga tccggtggag atccccagat
     8041 gcggctgttt gttctgccgt gaggagagaa gcaccgcatc cagttgttgc gccttaagcc
     8101 agtcgcgcag cgaagcgagt aatgtcatct ggattttcct tcttacaggc tatcgattaa
     8161 caatttgcct ttacggaaca tcatcagacg caggaacacc accatcagcg cagttatgac
     8221 tgcccccagc gcgatccccg ccatatagac gcccaggtta gtgaccagcg gccatgccca
     8281 gatagcggat tccgggaacc attgcactgc gcccagccag acagcggcgg ttgagccgac
     8341 aatcgcgccg accatatacg acggaatggc ggtgatgggg ctttccagcg caaacggaat
     8401 cgcaccctca ctgatcccca taaaggcaag gaacatcgcg gtcttaccct gcggataaag
     8461 ttgtgcgttg aacaggcgtt tgccggttaa acgacggtca ataatggtcg ccagacccag
     8521 accaatcggc ggaataacga tagcgataga acgagcggtg accggtaata cgtggtcagt
     8581 ggtaaagctg aaggcaacaa aacctgcggc tttgttgatc gggccaccaa ggtcgattgc
     8641 tgtcgccgcg gcgatcccca ttgcgtacat cagcgcgccc ttctcacctg cggcagtcag
     8701 cacagtacgg ataccgccgt tgatccagcc cccaaagggg gtgatgacgt agtacatcgc
     8761 cagcatgaca aaaatagcgg aaagaatcgg tagcaaaaat gtggttttga acgctaacag
     8821 gaaatccggt aactggattt tctggttcat ccatttcaca aggtaaccgg caacgataga
     8881 aataatcagc gcaccaatga aggtggacgg caccggcgaa gaggtcgccc attgcatagt
     8941 gctgggatcg aagttcagca gttgcgtcgg ctgggtagac atcaagccgc cgataaaacc
     9001 agccggaaac gccagtttgc caccgataga gttcgccaca aaagcagcaa acatcggaat
     9061 ggcaaaacca aacaacacgc cgccgaagga ctgcgacaac caggcaaatt tcagtaagga
     9121 gaggtcgaaa ccggagaatt tcccgctatt aagagcatcc atgatgccga tctcagcagg
     9181 aattttaagc cagctataag caatcaactg gctgaaagcg aggatcacac cgcccataat
     9241 taaggtcggc accatgcgtg aaatccctga catcacatgc tgcgggagtt caccccagaa
     9301 gctgctttta gaggcctgcg gattcttaac tgccgctgcc gcaccggatg cgccaggcac
     9361 aacggttgca ctgcgttttt taatggccat aacaattatc cctgtcgaat attactgttg
     9421 ttcagaggca atcatctctt cgatttcttt gatgatgccc gcagcgtttt taattgcgtc
     9481 ctgcaaagtg atttcataaa cgtcgcgtga ttcgaaacgt tcgttatctt ccggggtaac
     9541 tgccacggag tggatgatga tggtcgcttc ggcgatatcc tgcgccgtca ggcgattctg
     9601 gataccgtcc gcgccctggg tttcaatttt cacttcataa ccggcttcta ccgccgcttc
     9661 ttccagcgcc tgagcggcca taaaagtgtg agccaggccc attgggcagg cacataaggc
     9721 aatcagtttc ttactcatcg ttaattcctc gttcagtgat agttgacgag tgaaattatg
     9781 aacgtgcaaa acaaatcgcc gttaccagac aaataatgca tttactggag aattaatccc
     9841 gctgtacgga agtgtgacgc aggtcggcct tgtgagggga cacaaagaaa agttgaatta
     9901 actgtatgat ttaaaagatt atcgggagag ttacctcccg atataacagg aaggatttac
     9961 agaatgtgac ctaaggtctg gcgtaaatgt gcaccggaac cgagaagacc cggattgtca
    10021 tggacgatga gatacaccgg aatatcatgg acatattctt taaagcgccc tttatcttca
    10081 aatgcggcac ggaaaccaga ggctttgaag aactcaagga agcgcggcac gataccgccc
    10141 gcaataaaca cgccgccaaa tgtcccgaga ttgagcgcca gattgccgcc aaaacggccc
    10201 ataatgacgc aaaacagcga caacgcacgg cggcaatcgg tgcagctgtc agccagcgcg
    10261 cgttcggtaa tatcttttgg cttgagattt tctggcaggc ggttgtcggc tttaacaatt
    10321 gcgcgataca aattcaccag tcctgggcca gaaagcacgc gttccgccga aacatgacca
    10381 atttccgcac gcaatatttc gaggataatg gcctcttctt cactattcgg cgcaaaatca
    10441 acgtgaccgc cttcgcccgg caagcttacc caacgcttat cgacatggac cagatgcgca
    10501 accccaagcc ccgttccggc accgtaaacc gcaataggct taccttctac cggttctgcg
    10561 ccaccaaact gaatcagatg ctcttttttc agcatcggga tcgccatcga tacagcggta
    10621 aaatcgttaa taatttccag atggctaaaa ccgagattct ttttcatttc ggcaattgag
    10681 aacgcccagg tatggttggt cattgccacc cagtctccgg taatcgggca agcgatggca
    10741 atacagccgt ctttcacctc gaccttatgt tcttcaagat aaacgcgaat gaccgcttcg
    10801 aggctggggt aatcaagccc tgaataggtc ttagcctgcg agatttcacc actggcaata
    10861 tcacacagag caagacgtgc gttggtgccg cccacatcac cgactaatgc atactttgtc
    10921 attcttcaac tgctccgcta aagtcaaaat aattctttct cacactgtaa atacctgggg
    10981 gcataacaac aacgccggaa aggcaggctc cctgtaaata tcgatctggg tcacacaatt
    11041 actttatcgt ttcagcacca attgcagcga tgcctttttg caagctgggc aaactaagta
    11101 tctgaccccg cataaggaat agaacatgct ccatccgcga gccagaacca tgttgttatt
    11161 atcgctcccc gccgtggcaa ttgggattgc gtccagtctt attctgattg tggtgatgaa
    11221 aatcgcctcg gtattacaga atttgctctg gcaacgactg ccgggaactc tggggatagc
    11281 ccaggattca cccctctgga tcatcggtgt attaacgcta acgggtattg cggtggggtt
    11341 ggttatccgt ttcagccagg gtcatgccgg accagacccc gcctgtgaac cgctgatcgg
    11401 cgcaccggtt ccgccctctg cgctaccagg acttatcgta gcattaattc tcggtcttgc
    11461 tggcggcgtc agcctggggc cggaacatcc gatcatgacc gtcaatatcg cccttgcggt
    11521 tgccattggc gctcgtctgt taccgcgcgt caaccgaatg gagtggacta ttttagcctc
    11581 tgccggaacc atcggcgcgc tgtttggtac acctgttgcg gcggcgttga tattttcgca
    11641 aaccttaaat ggcagtagtg aagttccgct atgggatcgt ctttttgcgc cgttaatggc
    11701 ggcagcagct ggtgcactta ctaccggatt atttttccat ccccattttt cactgcccat
    11761 tgcgcattac ggacagatgg aaatgaccga tattctcagc ggtgcaattg tcgcggcgat
    11821 tgccatcgca gcagggatgg tcgccgtatg gtgcttacca cggttgcacg cgatgatgca
    11881 tcaaatgaaa aatccggtgc tcgtgctggg tattggcgga tttattctcg gtattctggg
    11941 ggttattggt ggaccagttt cgctgtttaa agggctggat gagatgcagc agatggtggc
    12001 aaatcaggct ttcagcacca gcgattactt tttgctggcg gtaattaaac ttgccgccct
    12061 ggtcgttgct gccgccagtg gctttcgcgg tgggcgaatc ttcccggcag tgtttgtcgg
    12121 cgtggcatta gggttgatgc tgcatgagca cgttcccgcc gtaccagcgg caataaccgt
    12181 ttcctgcgct attctcggca tcgtgctggt ggtaacacgc gatggctggt taagtctttt
    12241 tatggcggca gtcgttgtac ccaataccac attgctaccg ctgctctgta tcgtcatgct
    12301 tccggcatgg ctgttattag caggtaagcc gatgatgatg gtcaatcgtc cgaagcaaca
    12361 gccaccccac gataacgttt agcaaaaaat gctctccttt atgatttaag agttatggcg
    12421 atgatacggg gctttacctc cccgtaatat tgcgttaaca ggccgctgac ggcttcgatc
    12481 gtgaaggaga ataacaatgt tcaggtcact gtttctggcg gccgccctga tggcatttac
    12541 cccgcttgca gcaaacgcag gcgaaatcac cctactgcca tcaatcaaat tacaaattgg
    12601 cgatcgcgat cattacggta attactggga cggtggtcac tggcgcgacc gtgactactg
    12661 gcatcgcaat tatgaatggc gtaaaaaccg ctggtggcgt catgataatg gctaccatcg
    12721 tggctgggat aagcgtaaag catatgagcg tggctatcgt gaaggctggc gcgatcgtga
    12781 cgatcatcgc ggaaaaggcc gcggacatgg acaccgccat taaacacatt cgccaatgga
    12841 gcacaaggcc tgatgcgacg ctagtgcgtc ttatcaggcc tacaaaccgg ctctttaacg
    12901 cgtagggcgg ataaggcgtt tacgccgcat cgccaatagt gccagatgcg acgctcattc
    12961 aactacaatc ccagcgccgt ccccaccaat aaccagatat tcagcgccac gaccagcact
    13021 acaatcaccc agcctgtctg ttttacgcgt ttgctgttca ccagatcgcc catcaacttg
    13081 ctgtcactgg tgaaaatcag cagtggaacc agcgccaggg cgataccaaa acttaacagc
    13141 acctgactca taaccagaat ccgtgtcgga tctaatccca tcagaatgac aataaatgac
    13201 ggcagcatgg tgactgtacg acgcacccac agcgggatat ggaagcgaat gaacccctgc
    13261 atcaccacct gccctgccag tgtccccacc accgttgagg acagtccggc agcaaccaga
    13321 cttaacccaa agaccgttgc cgcagcatgg cttaacagcg gttgcagcgt caaataagcc
    13381 tcatcaagat cggcaacacc agtatgacca gaaaagtgga acgccgccgc agctgtagcc
    13441 atcatcgcca gattgacaaa accggcaata gtcatggcga tagccacatc ccatttggtg
    13501 gcggaataac gttgttgacg cgaaccgcca tgtaaatgct gagtgagcga agagtgcaaa
    13561 taaatcacat gcggcataat cgtcgcccct aacacgcctg ctgccaggaa gaccgcctcc
    13621 gaagtaggta aactcgggat caccattcct ttacccagct gcgccaggtt aggctgggag
    13681 aaaatcaact cgacaatgta agccgcggca acaaacaaca gtaacccgcc aatcactttc
    13741 tccagcggtt tttgcccgcg acgttgcagc attaaaatca ggaaagtcgc gatccccgtc
    13801 agcaccgcgc cctgcaacaa cgaaacacca agaatgagtt taaaaccgat cgccgcacca
    13861 ataaattccg ccaggtcggt tgccatcgca ataatttctg cctgaaccca atagaaccac
    13921 actacgggac gcggatagtg atcgcgaatc tgctccgcca gatttttacc ggtggcaatc
    13981 cctagtttgg cagagaggat ctgaatcagc atcgccatca ggttggccca aacgacaacc
    14041 cacagtagct gatagccgaa gctggcaccc gcctgaatat tggtcgcaaa gttaccggga
    14101 tcgatataac caatcgccgc aatgaacgca ggtcccatta atgcgagcct catcttgcgc
    14161 gccgcccgtc cgctgctact ctcaacgcga tagttcgtca tcttgtgcct ctaaaacata
    14221 gcctttgcta tgtttcatgc tatgccaaac gagaatgatt atcaaattca tttaaatggg
    14281 ttgtggtgat ttctctgata gaccaggatt atgactacga aaagattgat gacttgaatg
    14341 ttatggttat gtttaatgtt agcacattta cataactttc agcttccata cacaacatag
    14401 cagaaatgta tgacagctca ctatttttga agcttgtcac aggacgtcat tatagtgtgt
    14461 gtcagatctc gttttcctta accatgttac atagaatgtg cacggaaatt taacctgcct
    14521 catatttgga gcaaatatgg accgcgtcct tcattttgta ctggcacttg ccgttgttgc
    14581 gatcctcgca ctgctggtaa gcagcgaccg caaaaaaatt cgtatccgtt atgttattca
    14641 actgcttgtt atcgaagtgt tactggcgtg gttcttcctg aactccgacg ttggtttagg
    14701 cttcgtgaaa ggcttctccg aaatgttcga aaaactgctc ggatttgcca acgaagggac
    14761 taacttcgtc tttggtagca tgaatgatca aggcctggca ttcttcttcc tgaaagtgct
    14821 gtgcccaatc gtctttatct ctgcactgat cggtattctc cagcacattc gcgtgttgcc
    14881 ggtgattatc cgcgcaattg gtttcctgct ctccaaagtc aacggcatgg gcaaactgga
    14941 atcctttaac gccgtcagct ccctgattct gggtcagtct gaaaacttta ttgcctataa
    15001 agatatcctt ggcaaaatct cccgtaaccg tatgtacacc atggcagcaa cggcgatgtc
    15061 caccgtttct atgtccatcg ttggtgcata catgaccatg ctggaaccaa aatacgtcgt
    15121 tgcagcgctg gtactgaaca tgttcagcac ctttatcgtg ctgtcgctga tcaaccctta
    15181 ccgtgttgat gcctgtgaag aaaacattca gatgtccaac ctgcacgaag gtcagagctt
    15241 cttcgaaatg ctgggtgaat acattctggc aggtttcaaa gttgccatta tcgttgccgc
    15301 aatgctgatt ggctttatcg ccctgatcgc cgcgctgaac gcactgtttg ctaccgtgac
    15361 tggctggatt ggctacagca tctccttcca gggcatcctg ggctacatct tctatccgat
    15421 tgcatgggtg atgggtgttc cttccagtga agcactgcaa gtgggcagta tcatggcgac
    15481 caaactggtt tccaacgagt tcgttgcgat gatggatctt cagaaaattg cctctacact
    15541 ctctccgcgt gctgaaggca tcatctctgt gttcctggtt tccttcgcta acttctcttc
    15601 aatcgggatt atcgcaggtg cagttaaagg cctgaatgaa gagcaaggta acgtggtttc
    15661 tcgcttcggt ctgaagctgg tttacggctc taccctggtg agtgtgctgt ctgcgtcaat
    15721 cgcagcactg gtgctgtaag accatacata aaaaagccgg ggataattcc ccggcttttt
    15781 tacgcctgtt aatcagctaa tggctgcggg cgaccaatca aatacccttg cagatattgc
    15841 accccgagct tatgcaatag cgcctgttgc tgttgcgtct cgacaaactc cgcgaccaca
    15901 ctcaatgact tcgctttcgc cagatcggta attgatctca caatcatcgc atccagcgta
    15961 ttcgtgacaa tatctttcac aaagacgcca tcaattttga tgatatcagc ctgcaaacgc
    16021 tttaaccgtt cgtagttggc atagccggtg ccaaagtcat caatcgcaat ccggaaacca
    16081 aacttatgca gctgctcgat gttgtacatg ctgctttctg cgttagaaaa cgcctgctcc
    16141 tcggtgatct caagaatgac cgcctgcggg gagatgtgat aacgcttaaa tagacgaata
    16201 atccgcccgg caatattctt ttgcagcagc gtgagcggca ttaaattaac tgaaaagcgc
    16261 gggccttttt tgtcgcaagg gtgtgttgcc agccacttca acaaggattc cagcacttgc
    16321 aaatcaaaac gcgcgctaag gttaaactga gcaataaggg gcagaaactt atccggggtc
    16381 ataatgccgc cgtcatattt cagtcgcgcg aggacctcat cataaccttc accctctttg
    16441 ttgcgaattg gctgggcgta gagcagcaaa tcaccctgat ctaacgccgt gcgaatggta
    16501 ttcagcaata gcacctgttt agtggtctgc ccggaaacca tctcctctcg gctatccagc
    16561 gccagcacat gatgatgtgc gcaggattgc tccgccagcc agcttaactg ccccaacaag
    16621 ggttgcaggg tttcctgatt tccatcaaaa cgcccccagg cagcaccata gcccatatcc
    16681 agcccggtat tgttccagtg aatttgccga ctattcagga tgttaaccat atgctggagt
    16741 cgcccttccg tttcaggccc gctcagcact aacagcagtt cactacccgg caattgatac
    16801 aacttttcgt tttcctgcat caatggcagc agcgtacggt aaattgatcg gatacagtga
    16861 acacgcatca tcaacccgta atgacgactc ataaactcaa gattatcaat gcgcaggcag
    16921 caaaaactct tgcccgcctc ttgctccggc gcttgttcca acgcacgaaa gttgggcagg
    16981 agcgttaacg gatcggtcag cgcctgcaaa tgccagcggc gattaagcca ttcactgcga
    17041 tgataaatgc gcaccatata gagcaggcaa acgctaaagg aaatcagcac cgcgagaata
    17101 aatgccagcg aatattcggt ttccacccct tgcaaaaagt tctggttgta attcagcagg
    17161 caaagcgttg aaacagccca agtgagattt aaaaacggat agcgaagctt accgaccccg
    17221 agggtaaaga tgatgaagaa aacgggtacc aggtaaccgg caataaagtc gttttcataa
    17281 ggtgtgcaca acagaagtag cagcacgctt aacgctgcca gccagcttaa ggtaaatgcg
    17341 cgtttctctt tgcccaacga cggagcgata tccctgcgcc acaatatctg cgcaaagtgg
    17401 ggacttacaa tcatgcgagt gagatagtag aaaagcatgt tgtaaatcag cacagcggtg
    17461 aaaaggctta gcaaatcaac gaccgtgaaa atggcatccg catcgccgaa aaaggtggat
    17521 atctttagcg ggaaatcaaa gaagctcccc acaagataca tgctgcattt gatgccgatc
    17581 ggcgtcacca aaccaagcca gaacagacgt tgccagacat atcggctggt caatccataa
    17641 cgccaacgcg tccccagctg ccagcgtaat attgcgcagg cacacaacac gacaaacgtc
    17701 tggctaccga gcatgaccgc agtctgtaaa aaagagagat gaaagttcca cagattggtg
    17761 caaaacatcc cgcataaaac gggaacgaca ccgcgccagc caaagataaa caagattgac
    17821 agcattacgc acagtggcat ccacgccaga aagatataac tggaatggat aatggcaagt
    17881 ggcgaaataa aacgggatag ctgaataagt accacggtga gcgtaaacgc tagtgtgaat
    17941 atcttgatat tttttatcag gttatgctcc acaaacatga gtacactact ctcactgatc
    18001 ggggttttca ggcacacaaa taataagttt attcgcattg cgaagcaaac aggggccatc
    18061 ctgttgccga tttttcatcc aatgatgctt tcaccaggcg gcactaaccc ggacgtagac
    18121 tgtattacaa aagcggcaaa aagcagagac aaaaaacccc cgctttgcag cgagggttgg
    18181 aaatttggtg gagctaagcg ggatcgaacc gctgacctct tgcatgccat gcaagcgctc
    18241 tcccagctga gctatagccc cacgatgcgt ttacgtacca aatttgctgg gtgcaaaatt
    18301 tggtggagct aagcgggatc gaaccgctga cctcttgcat gccatgcaag cgctctccca
    18361 gctgagctat agccccgtca cgtaaagctt gtcgagttga cgggcggcat catatgaatt
    18421 ccgcccgaat gtgtcaacgg caaattgcaa cgtgtagttt caatcgctga aaaatcaggc
    18481 aaatgaacaa ttttggaaaa ccgctcgcat tcagtagtta ttcatgtcac ggtttcctgt
    18541 aaagtggtgc tataaaatga actactaata gacccacata cattcaggga attgttatgt
    18601 tcaaggagag gatgacacca gatgaacttg ccagactgac cggctatagc cgccagacca
    18661 ttaataaatg ggtacgcaag gaaggctgga caacgtcacc aaaacctggc gtccagggtg
    18721 gcaaagcccg actggttcac gtcaatgaac aggttcgtga atatattcgt aatgctgaac
    18781 gtccagaagg tcagggagaa gcgcctgccc tttctggtga tgcgccgctt gaagtattgc
    18841 tggtgacact ggcgaaagag atgacgccag ttgaacaaaa acagtttaca tccttgcttc
    18901 tgcgggaagg gattatcgga ttgttacaac gcttagggat tcgcgatagc aaataatatg
    18961 aaaagattac gcaataaaat gaccaccgaa gaactggctg aatgcctcgg tgtggctaaa
    19021 caaactgtta accgttggat cagagaaaaa ggctggaaaa cggaaaaatt tcccggcgtg
    19081 aaaggcggtc gtgccagact tattctggtc gatacgcaag tttgcgagtt tattcagaac
    19141 acgccagcct tccataacac accgatgctt ctggaagccg aagaacccct tgccgaatac
    19201 gcaccgggga ttcgcactcc tgcttatcgg caaatcatca gtgcaataga caacatgact
    19261 cacagtgagc aggaaaaagt tgcgcaattt ttgtcacgcg aaggaattcg taatttcctt
    19321 gctcgtctcg acatagatga atcagcttga catcctccac gccctgaatg acgaggatcc
    19381 ctgctacgtt caggctgtcg cctgaatcat ttcggtgggt tcctgcttca acgagcggcc
    19441 tgactgcacc atctctccac aggcaagaac ggcgtgcccc gccgctaaaa tgttacgagc
    19501 gccgtttaca tcggcgttcg ctgtatatcc acatacctgg catctgaatt tactttgtga
    19561 caggcggttt tctttcgctg tatgaccaca gcacgcgcaa cgctggcttg tgtacgctgg
    19621 cggaacagcc agtacctgac cgccacgcca gagctgcttg tactcaagct ggcggcgcat
    19681 ttcataccag ccctgatcca gtatcgaacg gtttaaacct gattttgccc ggacattgcg
    19741 ccccggctgg ctgaccgtac ccgctgctga ctttgacatg ttgctgacct tcaaatcctc
    19801 aatgactatc attgcgtggt ttttgctgac ggtcgttgtg actttgtgaa ggtagtccct
    19861 gcggatattt gcggtacagg aatgcagtcg ctgtattttg cgtttctgct tctgccagtt
    19921 attgctgaat ttgaccttgc ggcttaactg tcgctgaagt ctcgccaact tcttctggtt
    19981 tttctgaaaa ctgtttacag gctcaaagac tgtgccatct gacagcgtgg cgagtttagc
    20041 cacgccagca tccagtccga ccattgatgc tgaagggtga accggagttg atacttcact
    20101 ttctgtctga atactgatgt accacttacc gcaggactgg ctgacagtga catttttcac
    20161 aacacccgtg acctgacggc tgttcaggta gcgcatccag ccaagtttcg gcagaaaaat
    20221 acggctgttt tcctgatcga gcttaacgcc ctgcgggtag cggaatgcat cattctgtcc
    20281 ccgctttttg aatcggggaa aagcagcccg cttccggaag aagtttttgt aagcccgctc
    20341 aagctctttc agtgactgtt gcaatggctg tgacggggca tctttaagcc attgcgtttc
    20401 agtggcgttt ttccactcaa ccagccagga agccattttc ccgtaaggga tgtatttatt
    20461 accggcctca tgattctcat tctgaagcgc cagagcacga ttgaaaacga aacgacatgc
    20521 gcctgcgaag cgcctcattt cacgctcctg ttgaccacct ggtcttaact ggaatttaaa
    20581 tgcctgtagt cgtttcacag gccaaatgat actttggtct atgaataaag aaaccgatat
    20641 ccgacgggga aggcattgtg ttttcctgat gcatgttcat ctgttctttg tcaccagata
    20701 ccgacgtcag atttttgact atgacgcgac agaaaaacta cgcacttact tttcaaatgt
    20761 atgtgctgat tttgaagctg aactggttga aatggatggc gaaccagatc acgtccattt
    20821 gttaactaac tatcctccca aactggcgat atccagtctg gtaaacagcc tcaaaggcgt
    20881 atcgggtagg ttactgcgac gagatcgacc agatattgca gtcaggtatt actacaaagg
    20941 cgttttgtgg agtcctggct attttgccag tagctgcgga ggtgcgccaa tatccgtcat
    21001 ccgccaatac attgaacaac agcaaacacc tggtcaggtg gaaaaccgcg ccttatatcc
    21061 ccgccctgaa ggacggggtt ttacggcgca ccggataaat aaaaaacggc aggggtatct
    21121 ctcctgccgt ttatctttta tgcgctaatt actgctgatt ttcgcgttcc gcaataaaag
    21181 ccagcgcttt gttgatacgc tcgatactgc gggtcttacc gatcgcgtga acggtaacgt
    21241 ccagcgccgg agactgcccc gcaccggtta cggcgacacg cagcggcata ccaactttac
    21301 ccatacccac ttccagctca tccgccgtcg cctgaatagc gtgatgaacg ttttcagctg
    21361 tccagtcagt aatcgcggcc agtttgtcac gaaccacttc cagcggctga cgcgctaccg
    21421 gacgcagatg ttttttcgcg gcgtcggcat cgaactcagc aaaatcttcg tagaaataac
    21481 ggcagctctg tgccatctct ttcagcgtct tgcagcgttc gcccagcagt ttcaccagat
    21541 cagccagctg cgggccgtta cgggtatcga tattttcctg ctcaatgtgc cactgtaagt
    21601 gagtagcaac atactccggc ggcagcgcgt taatgtagtg atggttcagc cacagcagct
    21661 tgtcggtgtt gaacgcactg gcagatttgc tgacggcatt caaagtgaag tatttgatca
    21721 tctcttcacg agtgaagatt tcctgatcgc cgtgggacca gcccagacgc accagatagt
    21781 tcagcagtgc ttctggcaaa taaccgtcat cacgatactg cattacgctg actgccccgt
    21841 gacgtttgga cagttttttg ccgtcatcgc cgttgatcat agaaacgtgc gcgtaaaccg
    21901 gtaccggcgc tttcagggct ttaagaatgt tgatctggcg tggcgtgttg ttgatatggt
    21961 cttcgccacg gataacgtgg gtgatttcca tatcccagtc atcgacaacc acacagaagt
    22021 tataggttgg ggaaccatcg gtacggcgga taataagatc gtccagttcc tggttgctga
    22081 actcgatcgg accacggatc tgatcgtcaa aaacaacaga accttcctgc gggttagcaa
    22141 aacgtacgac acacggctca tcatcagcgt gatgctcatg actgtggcgg cagcgaccgt
    22201 cataacgcgg cttctcacct ttcgccattt gctcttcgcg cagcgcttcc aggcgctctt
    22261 tagagcaata gcatttataa gctgtgccct cttccagcat ctgatcgatc accgcgttgt
    22321 agcgatcaaa acgtttggtc tggaagtacg gaccttcatc ccactccagg ctcagccagt
    22381 tcatgccatc cataatggct tcgatagctt ccggcgtgga acgctcaaga tcggtgtctt
    22441 caatacgcag cacgaactca ccgccgtgat tacgtgcaaa aagccaggag taaagagcag
    22501 tacgcgcgcc gccaacgtgc agatagcctg ttgggcttgg cgcgaagcga gttttgattt
    22561 tcatgaaatg gccttacgtt tagaaagatg ccgacaaccg gcaaatcctg gaaaaattga
    22621 gtgggcgata ttctatcact cccgcctgat tcctcaatgt agttcgggtt gcaaccttac
    22681 gcattattgt tttcgttgac aaattgcgca acacggtctg ttttgcgatc atcttgctta
    22741 aatttacgac gaacgaacaa tttctttaga aaatgcgttg actcattttg aactctccct
    22801 ataatgcgac tccacacagc gggggtgatt agctcagctg ggagagcacc tcccttacaa
    22861 ggagggggtc ggcggttcga tcccgtcatc acccaccaac tactttatgt agtctccgcc
    22921 gtgtagcaag aaattgagaa gtgggtgatt agctcagctg ggagagcacc tcccttacaa
    22981 ggagggggtc ggcggttcga tcccgtcatc acccaccact ttctcaccag ctaaatttct
    23041 tgtaaaaatg tgaagtaccg aagtgggtga ttagctcagc tgggagagca cctcccttac
    23101 aaggaggggg tcggcggttc gatcccgtca tcacccacca cttcgggtcg ttagctcagt
    23161 tggtagagca gttgactttt aatcaattgg tcgcaggttc gaatcctgca cgacccacca
    23221 atgtaaaaaa gcgccctaaa ggcgcttttt tgctatctgc gatttgtaag attgattttt
    23281 cttctcaagt tccttgttct tctgccgata ttacctcgta cagtgacgag gactgaacaa
    23341 tggccactat ctcttcaact tcaattcctt ccatacaaac acagagtagt aacagggcat
    23401 cacaaggtag cgatgttgct tcacaaatcg cgcggataag tcagcaaatc atcaaactca
    23461 ctcaacacat taaagagatt gtagacacta gcggcagtgc tgaagacaag caaaaacagg
    23521 ccgaattgat tcaacagcaa atcaccctac tggaaacaca actggcgcag ttgcagagac
    23581 agcaggcaga aaaggcgcag gagaaagagc agcgtttgtc tctgaatgta tcccttctaa
    23641 atcctgttga aaatacgact catatcggta tctatatctg atacaacgga tccccttccc
    23701 gcccagccat tagcgtattg ccctggtctc gtaccacgtc ccataacgct tcagcggcgg
    23761 tggaaagtga acggtttttc ctacgcacca gcatcaactg gcgttccacc accggcgtaa
    23821 tgcgctttac caccagcggg ctaccttcag gcaatggtag cgccagtgcg ggaagaatac
    23881 tgatgccgat gcctgcggct accatcggga acagcgtcgc cggatgtcca atctcctgca
    23941 caatattcgc ctgaatacca ttgcgcgcca gcgctgcgtc aatcagcggt cggctgcctg
    24001 acgcataatc ctgcaacacc aatttcgctc cctgtaatgc ctgccagggt acataatctt
    24061 ccgcggccaa agcgctatcc cgatggcaaa gcagaaagaa aggctcggaa agaatcactt
    24121 cacattgcag atcgcccact gggcctggat cgatgacgat gccaaagtcg acatctccct
    24181 ggcgaatact ttccatcacc cactgctgcg gacgatcgtg caggacaaac tgaatatctg
    24241 gatagcggcg atggctttca gcaatgcatt gcggaataag atgcgcggaa atggtctggc
    24301 tggcagcgac ccgaactttt ccgctcagtt gttgccccat acgcccggta tcgcgcaacg
    24361 tgctgttaag ttcatccagc agtcgctcaa gacgcaaagc caactgctgc cctgcatctg
    24421 taagcaccac ttcacgcgtg gttctgtcca gcaggcgaac accagtatga ttttccagct
    24481 ccttcacact gtgacttact gccgactggc tcaggctgat acgctctcct gcacgactaa
    24541 aacttttctc ctgcgctact gtgacgaaaa cctttaattg ttttaaagaa taattcatct
    24601 gttttcttca tgaatggatg caataaatca attttatttc tcaaactgga agaagcacaa
    24661 tagaaccatc gatcatctgg agtctttatg aaactttttc gtatcctcga tcctttcacc
    24721 ttaaccctga tcacggtggt gttgctggcc tctttctttc ctgccagagg cgatttcgtc
    24781 cccttctttg aaaatctgac caccgcagct attgccctgc tgttctttat gcacggcgcg
    24841 aagttgtcgc gtgaggcgat tattgctggc ggtggtcact ggcgactgca tttgtgggta
    24901 atgtgcagca ccttcgtgct gtttccgatt ctgggtgtac tgtttgcctg gtggaaaccg
    24961 gtaaatgtcg acccgatgct ctactccggt tttctctact tgtgcattct cccggctacc
    25021 gtgcagtctg caatcgcctt cacgtcaatg gcgggcggta acgtcgcggc ggcggtttgt
    25081 tctgcgtcag catccagtct gctggggatt ttcctttcac cattgctggt tggtctggtg
    25141 atgaatgttc acggtgcagg gggcagcctt gagcaggtcg gtaaaattat gctgcaactg
    25201 ctgctgccgt ttgtgttggg gcatctttcc cggccgtgga ttggtgactg ggtgtcgcgc
    25261 aataaaaaat ggattgcgaa aactgaccag acgtccattc tgttggtggt ttatacagcg
    25321 ttcagcgaag ccgtcgttaa tggtatctgg cataaagttg gctggggatc attgctgttt
    25381 atcgtggtgg tcagctgcgt tcttctggct atcgtgattg tagttaacgt ctttatggca
    25441 cgccgactgg gcttcaataa ggcagatgaa attactatcg tcttttgtgg ttcgaaaaag
    25501 agtctggcaa atggcatccc gatggcaaac attctgttcc ccacatcggt gatcggtatg
    25561 atggtgctgc ccctgatgat tttccatcag atccaattga tggtctgtgc ggtgctggcg
    25621 cgtcgataca aacgccagac cgaacagtta caggcgcagc aggaaagcag cgccgataaa
    25681 gcttaaagcg gacgcttcag gggctggacc agttgcgtca gcccctcggt tttaatcagc
    25741 agcgtgattt gcattagctc accgagcttc cctgccggga actcatcttt gcgggcaaac
    25801 cacagcagat actcctccgg caggtcgatt aagcgacgcc ctttgtattt gccaaacggc
    25861 attatcgtat tggctatttc aatcagctgc tctttttcca tctcagctac ccagcaaacg
    25921 cagcatttcc gcttcgtcga tgacttcaat gcccagttcc tgcgccttcg ccagtttaga
    25981 tcctgcagct tcacccgcta tcaccagatc ggttttcttc gacacgctgc ccgcgacttt
    26041 cgcgcccagt tcgaccagtc gagctttagc gtcatcacgc gacatctggc ttaagctgcc
    26101 cgtaagcacc acggttttac cagcaaacgg gctgtcaatc tcttccgcgt tgataacgat
    26161 cggcgcaggc cagtgaacac cttccgccaa cagctcgctg atgacattgc ggttgctttc
    26221 ttcggcaaag aagttgtgaa cgtgggatgc aacgacaatg ccaacatcag gcaccttttg
    26281 cagctcttca atcgaagcgg cttccagcgc ttccagcgtt ccgaaatatg ccgccagacc
    26341 tgctgcggtg gcctcgccga cttcacggat gccaagtgca tagaggaagc gagcaaaggt
    26401 ggtttctttc gctttttcca gcgcgttaac cacgttttgt gccgattttg gccccatacg
    26461 ctccagtccg gtcagtttgc ctgcggtgag tttgaacaga tccgccggag tgtggacata
    26521 ttctttttca accagctgat cgatgatttt gtcgcccatt ccgtcaacat ccatcgcacg
    26581 gcgggaaaca aagtgtttca gcgactcttt acgctgcgca ccgcaaatca gaccaccagt
    26641 acagcgggca accgcttcac cttccacacg ctcaacgtca gaaccacata ccggacaata
    26701 cgtcgggaat acaacctcac gggtatcttc cgggcgttca gaaagcacga cgttaaccac
    26761 ctgcgggatc acgtcgccag cgcggcgaat caccacttta tcgccaatgc gtaaaccaag
    26821 acgttcgatt tcatccgcat tgtgtaaggt tgcgttactc accagcacgc ctgcaacatg
    26881 gacaggttcc agacgcgcaa caggcgtaat cgcgccagta cgcccaacct gaaactcgac
    26941 gtcacgcaca aaggtcatct gttcctgcgc cgggaattta aacgctaccg cccagcgcgg
    27001 ggctcgcgcg acaaagccaa gctgctcctg ctgttccagt gagttgacct taatcaccac
    27061 gccgtcgata tcaaagccca gcgtcgggcg gtcttcttcc actttgtgat agaacgccag
    27121 cacttcttcc gccgattcac aaagcgttac ccgatcgctg accggcaacc cccacttttt
    27181 aaattgcagt aaacggccaa gatgagtatc cggcagctcg ccaccttcca gaacaccaac
    27241 gccatagcag aaaaaagtga gcggtcgctt cgctgtaata cgcggatcaa gctgacgcag
    27301 tgaaccagct gccgcattac gtgggttagc aaacactttc ccgcccgtgc gtcgcgcatc
    27361 ttcgttaatc ttttcgaacc ccgcctgcgg caggaacact tcaccacgca cttccagacg
    27421 cgccgggata ttctctccgt gcaacttcag tggaatggcg cgaatagtac gcacattaga
    27481 cgtgatatct tccccggtgg tgccatcgcc acgagtcgcg gcactgacta aaacgccatt
    27541 ttcatacaga atactgacgg caaggccatc cagcttcagc tcacagcacc aggtgacttt
    27601 ctcgttgctt ttcagacggt cctgcacacg tttgttgaag gcaagaaagc tttcttcatc
    27661 aaaaacgtta tccagcgaca gcattggcac ttcatggcga atctggctga aagccgccag
    27721 cggcgcagca cctacacgtt gggtaggcga atcaggcgta atcagttctg gatgtttggt
    27781 ttccagctcg cgcagttcgc gcatcagcct gtcgtattca gcgtcgggaa tttccggcgc
    27841 atccatcaca tgataaagat attcatgatg gcgaagcgtc gttcgcagtt ctgtcagttg
    27901 ttgttcgatt gattccatat cgcaccatca atgctaaaaa cccccgacaa gcgggggttc
    27961 gaagaggagt taatttgcct taagtgtatc aggcgttggc gtctttaact tcgcggatga
    28021 tgtcctggta ctcgcgcaat ttctgcggag tcatcatacg gcgctgatcg tcaagcacga
    28081 caccgcccac ttcatcggca atatgctgcg cagattgcag catcagcttg aagttctgca
    28141 gctcgtcacc gtaagacggt acctgcataa agatagtgac acccggagta gtgaaatcct
    28201 tcatttcagg atcaaaggtt cccggtttca ccatattcgc caggctaaat aacgccgggc
    28261 cgctgccatc cgggctaaga tgacgatggt aaatattcat atcgccaaaa atgaagcccg
    28321 cttgttgaat gctgttaaga agcagttcac cgtttagctc gctaccgtga tgcgccgcga
    28381 cgttcatgat aatcaccgct tctttgcgct tcggtttatc cataactgga gccggttccg
    28441 ctacaggctc aggctgtggt gccgctacgg gttctgcagg ctggaaagcc tgttgtgccg
    28501 gttgcggtgc tgaatgcact ggctgcggcg ctggcgcgac ctgtggcgaa actggctgct
    28561 gcaacggctg ttcaggctgc ggctgatagg caggctgctg cacgggttgc tgcaccggct
    28621 gcgctggacg cggagcatgt tgcggcggta cctgcgcttc aggcggctgc tggaccggtt
    28681 gacgcggctg cgcagacgca taaggcggtt ggtactggtg ttgcggcgac ggacgagcag
    28741 cctcatgctc ctgggcgtta gccggggcat gattcacgcg gtgaacacga acctcaccaa
    28801 cgccctcatc atcttcgaca tcctcgtcat aagaatcgtc gtcacgtttt gacttcattc
    28861 gttttaatgg ccgatcgcgg aacatagaag atcgttcttt acggctggtc cagaaaccat
    28921 gtaccagtaa agcgattatg gcgatcgcgc caacaatgat taatatcaga cgcaaatcct
    28981 gcatcattat attctctgtt gttctaacac cttgccacca cggcaaacat ttactcacta
    29041 agagtatttg ccgattacct caagtgcaag tgcactatta actttcacag cacaaagata
    29101 gatgaaatcg tgctttttgc tgttttttcg aacatatcct aactgtccat tgcgcaatta
    29161 cccggtaaaa tacgcacaat tttcctggga ttggtcaaaa ggagctcatc ctgattatgg
    29221 tttcatcatt cacatctgcc ccacgcagcg gtttttacta ttttgcgcaa ggctggaagc
    29281 tcgtctcgca acctgggatt cggcgtttcg ttattttacc gctgctggtc aatattttgt
    29341 tgatgggggg cgcattctgg tggctcttta cgcaactcga tgtctggatc ccgactctca
    29401 tgagttatgt tccggactgg ttacaatggc tgagctatct gttgtggcct ctggctgtca
    29461 tctctgtgct gttagtgttt ggctatttct tctccacgat tgctaactgg attgccgctc
    29521 cgtttaacgg tttattggct gaacaactgg aagcacgatt gactggcgct acaccgccag
    29581 ataccgggat tttcggtatc atgaaagatg tgccgcgaat catgaaacgc gaatggcaaa
    29641 aatttgcctg gtatctgcca cgcgcaattg tattgctaat tctttacttc atccctggta
    29701 ttgggcaaac cgtcgcgccg gtactgtggt tcctgtttag cgcctggatg ttagccatcc
    29761 agtattgcga ttaccccttc gataaccaca aagtgccgtt taaagagatg cgcaccgccc
    29821 tgcgcacacg caaaatcacc aatatgcagt ttggtgcttt aaccagtctg tttacgatga
    29881 tcccgctgct taatctgttc attatgcccg ttgccgtttg tggcgcgacg gcgatgtggg
    29941 tcgattgcta tcgcgataaa cacgcgatgt ggcggtaaca atctaccggt tattttgtaa
    30001 accgtttgtg tgaaacaggg ggggcttatg ccgcccctta ttccatcttg catgtcatta
    30061 tttcccttct gcatatagat atgctaaatc cttacttccg catattctct gagcgggtat
    30121 gctacctgtt gtatcccaat ttcatacagt taaggacagg ccatgagtaa gatttttgaa
    30181 gataactcgc tgactatcgg tcacacgccg ctggttcgcc tgaatcgcat cggtaacgga
    30241 cgcattctgg cgaaggtgga atctcgtaac cccagcttca gcgttaagtg ccgtatcggt
    30301 gccaacatga tttgggatgc cgaaaagcgc ggcgtgctga aaccaggcgt tgaactggtt
    30361 gaaccaacca gcggtaatac cgggattgca ctggcctatg tagctgccgc tcgcggttac
    30421 aaactcaccc tgaccatgcc agaaaccatg agtattgaac gccgcaagct gctgaaagcg
    30481 ttaggtgcaa acctggtgct gaccgaaggt gctaaaggca tgaaaggcgc aatccaaaaa
    30541 gcagaagaaa ttgtcgccag caatccagag aaatacctgc tgctgcaaca attcagcaat
    30601 ccggcaaacc ctgaaattca cgaaaagacc accggtccgg agatctggga agataccgac
    30661 ggtcaggttg atgtatttat tgctggcgtt gggactggcg gtacgctgac tggcgtcagc
    30721 cgctacatta aaggcaccaa aggtaagacc gatcttatct ctgtcgccgt tgagccaacc
    30781 gattctccag ttatcgccca ggcgctggca ggtgaagaga ttaaacctgg cccgcataaa
    30841 attcagggta ttggcgctgg ttttatcccg gctaacctcg atctcaagct ggtcgataaa
    30901 gtcattggca tcaccaatga agaagcgatt tctaccgcgc gtcgtctgat ggaagaagaa
    30961 ggtattcttg caggtatctc ttctggagca gctgttgctg cggcgttgaa actacaagaa
    31021 gatgaaagct ttaccaacaa gaatattgtg gttattctac cgtcatcggg tgagcgttat
    31081 ttaagcaccg cattgtttgc cgatctcttc actgagaaag aattgcaaca gtaatgccag
    31141 cttgttaaaa atgcgtaaaa aagcaccttt tcaggtgctt ttttgtggcc tgcttcaaac
    31201 tttcgcccct cctggcattg attcagcctg tcggaactgg tatttaacca gactaattat
    31261 tttgatgcgc gaaattaatc gttacaggaa aagccaaagc tgaatcgatt ttatgatttg
    31321 gttcaattct tcctttagcg gcataatgtt taatgacgta cgaaacgtca gcggtcaaca
    31381 cccgccagca atggactgta ttgcgctctt cgtgcgtcgc gtctgttaaa aactggcgct
    31441 aacaatacag gctaaagtcg aaccgccagg ctagacttta gttccacaac actaaaccta
    31501 taagttgggg aaatacaatg ttccagcaag aagttaccat taccgctccg aacggtctgc
    31561 acacccgccc tgctgcccag tttgtaaaag aagctaaggg cttcacttct gaaattactg
    31621 tgacttccaa cggcaaaagc gccagcgcga aaagcctgtt taaactgcag actctgggcc
    31681 tgactcaagg tacagttgtg actatctccg cagaaggcga agacgagcag aaagcggttg
    31741 aacatctggt taaactgatg gcggaactcg agtaatttcc cgggttcttt taaaaatcag
    31801 tcacaagtaa ggtagggtta tgatttcagg cattttagca tccccgggta tcgctttcgg
    31861 taaagctctg cttctgaaag aagacgaaat tgtcattgac cggaaaaaaa tttctgccga
    31921 ccaggttgat caggaagttg aacgttttct gagcggtcgt gccaaggcat cagcccagct
    31981 ggaaacgatc aaaacgaaag ctggtgaaac gttcggtgaa gaaaaagaag ccatctttga
    32041 agggcatatt atgctgctcg aagatgagga gctggagcag gaaatcatag ccctgattaa
    32101 agataaacac atgacagctg acgcagctgc tcatgaagtt atcgaaggtc aggcttctgc
    32161 cctggaagag ctggatgatg aatacctgaa agaacgtgcg gctgacgtac gtgatatcgg
    32221 taagcgcctg ctgcgcaaca tcctgggcct gaagattatc gacctgagcg ccattcagga
    32281 tgaagtcatt ctggttgccg ctgacctgac gccgtccgaa accgcacagc tgaacctgaa
    32341 gaaggtgctg ggtttcatca ccgacgcggg tggccgtact tcccacacct ctatcatggc
    32401 gcgttctctg gaactgcctg ctatcgtggg taccggtagc gtcacctctc aggtgaaaaa
    32461 tgacgactat ctgattctgg atgccgtaaa taatcaggtt tacgtcaatc caaccaacga
    32521 agttattgat aaaatgcgcg ctgttcagga gcaagtggct tctgaaaaag cagagcttgc
    32581 taaactgaaa gatctgccag ctattacgct ggacggtcac caagtagaag tatgcgctaa
    32641 cattggtacg gttcgtgacg ttgaaggtgc agagcgtaac ggcgctgaag gcgttggtct
    32701 gtatcgtact gagttcctgt tcatggaccg cgacgcgctg cccactgaag aagaacagtt
    32761 tgctgcttac aaagcagtgg ctgaagcgtg tggctcgcag gcggttatcg ttcgtaccat
    32821 ggacatcggc ggcgacaaag agctgccata catgaacttc ccgaaagaag agaacccgtt
    32881 cctcggctgg cgcgctatcc gtatcgcaat ggatcgtaaa gagatcctgc gcgatcagct
    32941 ccgcgctatc ctgcgtgcct cggctttcgg taaattgcgc attatgttcc cgatgatcat
    33001 ctctgttgaa gaagtgcgtg cactgcgcaa agagatcgaa atctacaaac aggaactgcg
    33061 cgacgaaggt aaagcgtttg acgagtcaat tgaaatcggc gtaatggtgg aaacaccggc
    33121 tgccgcaaca attgcacgtc atttagccaa agaagttgat ttctttagta tcggcaccaa
    33181 tgatttaacg cagtacactc tggcagttga ccgtggtaat gatatgattt cacaccttta
    33241 ccagccaatg tcaccgtccg tgctgaactt gatcaagcaa gttattgatg cttctcatgc
    33301 tgaaggcaaa tggactggca tgtgtggtga gcttgctggc gatgaacgtg ctacacttct
    33361 gttgctgggg atgggtctgg acgaattctc tatgagcgcc atttctatcc cgcgcattaa
    33421 gaagattatc cgtaacacga acttcgaaga tgcgaaggtg ttagcagagc aggctcttgc
    33481 tcaaccgaca acggacgagt taatgacgct ggttaacaag ttcattgaag aaaaaacaat
    33541 ctgctaatcc acgagatgcg gcccaattta ctgcttagga gaagatcatg ggtttgttcg
    33601 ataaactgaa atctctggtt tccgacgaca agaaggatac cggaactatt gagatcattg
    33661 ctccgctctc tggcgagatc gtcaatatcg aagacgtgcc ggatgtcgtt tttgcggaaa
    33721 aaatcgttgg tgatggtatt gctatcaaac caacgggtaa caaaatggtc gcgcctgtag
    33781 acggcaccat tggtaaaatc tttgaaacca accacgcatt ctctatcgaa tctgatagcg
    33841 gcgttgaact gttcgtccac ttcggtatcg acaccgttga actgaaaggc gaaggcttca
    33901 agcgtattgc tgaagaaggt cagcgcgtga aagttggcga tactgtcatt gaatttgatc
    33961 tgccgctgct ggaagagaaa gccaagtcta ccctgactcc ggttgttatc tccaacatgg
    34021 acgaaatcaa agaactgatc aaactgtccg gtagcgtgac tgtgggtgaa accccggtta
    34081 tccgcatcaa gaagtaattc ttgctgcagt gaaaaatggc gcccatcggc gccatttttt
    34141 tatgcttccg ccagcggcgg caaaatcaat tcatcgctct catgctgctg ggtgtagcgc
    34201 atcacttcca gtacgcgcaa ccccgcgcgg tgcaccgcat cgtttaatgc cttccctttc
    34261 agcaaaccac tgataagctg agcacaaaac aggtcgccag tccctttcag gtcggttttt
    34321 acccttgaat gggaaatgac attcacgctg tcggcagtga ccaccacaac cagcatctcc
    34381 tgattttctt cattaccgga ggcgctggta atcaccaccc attttagtgt gtctgaaagc
    34441 agactttttg cggcagcaat ggcactgtcg agatcgcggc aatttttacc ggtcaggatt
    34501 tccaactcaa agatattggg ggtaattccc tgcgccagcg gcagtaaata ttgtcgatac
    34561 gcttcgggaa ggtcaggttt gacataaatt ccgctgtcaa tatcgccaat caccggatcg
    34621 accatgatca ataggtcagg atggtctttg cgtagcgcag tcagccactc ggcaaggatt
    34681 ttgatttgcg atgccgttcc catatagccc gtggttacag cacgaagttg gcgcagcgca
    34741 tcacgctcct gaagtgcacg caaatagccg ctaaaccatt catccggaat cgcaccaccg
    34801 tagaaagtgt cataatgcgg cgtattgctc agcaataccg tcggcacggc aaagacattc
    34861 aggccgttct gtttgatagc aggcacggca atgctgttgc ccacgctgcc gtaaaccacc
    34921 tgcgactgca cggcgacgat atccgcctgc agcgccctgc tcttatcgtt aaacaacaac
    34981 aaactactca tttaattttt tctccttgcc gatgatcctc atcgtattcc aaccgaaact
    35041 ttacctgatt ctggcagtca aatcggctat tacaaaacaa ggataaggta attcagtgaa
    35101 gaaaatcatt tgtctggtca ttacactact aatgacactc cccgcttacg cgaagttaac
    35161 tgcccatgaa gaagcccgca tcaacgccat gctggaggga ttagcacaga aaaaggattt
    35221 gatatttgtg cgcaacggtg atgaacatac ctgcgatgaa gcggtttctc atctgcgtct
    35281 gaagctgggc aatacccgta accgcattga cactgccgag cagtttattg ataaggttgc
    35341 ttcgtcgtca tcgattaccg ggaagccgta tattgtgaag atccccggta agagcgatga
    35401 gaatgcacag cctttttaca tgcgttaatt gcgcagacgg ataaaacggt gcctgcgcaa
    35461 taatcttaaa tccccgcccc ctgactaaaa tgctcttccc caaacacccc ggtggaaagg
    35521 tagcgatcgc cacgatcgca gatgatcgct accaccaccg caccagggtt agcttttgcc
    35581 acccgcagtg ctccggcaac cgcgccgccg gagctgacgc cacagaatat tccttcccgc
    35641 accgccagtt cgcgcatggt gttttccgca tcgcgctgat gaatatccag cacctcatct
    35701 accagagaag cgttgaaaat ccccggcaga tattccgcag gccagcggcg aatgccggga
    35761 atgctactgc cctcttccgg ctgcaggcca acaatggtca ccggtttgga ttgttcgcgc
    35821 ataaagcgtg aaacgccggt gatagtgccg gttgttccca tgctggagac aaaataggta
    35881 atgcgcccgc cagtctgctg ccagatttcc ggcccggtag tggtgtaatg ggcgtaaggg
    35941 ttatcgggat tattgaactg atcgagcagc tttccttcac cacgatccgc catctccagc
    36001 gccagatcgc gcgcaccttc catgccctgc tctttggtga ccagaatcag ttccgcacca
    36061 taagcacgca tcgccgcacg gcgttcctgg ctcatgttgt cgggcatcag caatttcatg
    36121 cgatagcctt taagcgcggc aatcattgcc agcgcaatgc cggtgttacc actggtggct
    36181 tcgattaaca catcacccgg tttaatttcc ccgcgctttt ccgcctcgac gatcatcgaa
    36241 agcgccgcac gatcttttac cgaacctgcc gggttattgc cttccagttt taaccacact
    36301 tcactgccgt tattcggccc cattcgctgc aacttcacca gaggcgtatt gcctattgtt
    36361 tgttctaatg tactcacgat ctctatccat acgtggtgtt gcctgatgcg acgcttgcgc
    36421 gtcttatcag gcctacaggt tacaaacctt gccattaaaa agcccggatc gcgggaagcg
    36481 cctccgggcg tttaacattc actcaaccta tcaggcgctt tgtgcgagag caagttcctc
    36541 atctcggggt tcgatacgct cgtcgccgtt atacagccgc gcatgttgca gaccaacgaa
    36601 taaacgctcg ccacgctgcg gggcatcgtc gccatgcatc acgaccgtca gcggttcgtt
    36661 gtaccacccc agcggctgca ccactaattg ggtgtagtga cctttcgggc tggcttccag
    36721 tacctgtacc ggcagcggcg aatcgaggct ggtacggcgg ctgatatcca cttcccacgg
    36781 gcgcaggaag agatccaccg gcccctgata cgcaggtgtg tagcccagcg gccagcgatg
    36841 tgcgccaaca tggaactgcc cgccgcgaat ggttccctgc aggcggttca cttcgcccat
    36901 aaattcgagc acaaaacggg tcgccggttc gcgccatacc tgatccggcg cgtcagcctg
    36961 ttcaatattg ccctggctca tcacaactac acgatcagct acttcggtcg cttcttcctg
    37021 atcgtgggtc acaaaaacgc tggtgaattt tagttcttca tggagttgac gcagccatcg
    37081 acgcagctct ttacgcacct gcgcatccag cgcgccaaac ggttcatcaa gcagcagaat
    37141 ttgcggttct acagcaagcg cacgcgccag cgctacacgc tgtttctggc cgccggaaag
    37201 ctgcgccgga taacgatccg ccagatgggc aagctggacc atttccagca attttgtcac
    37261 tttcgctttg atggctgcgg cattcgggcg ctcgcgacgc ggcagcaccg tcaggccaaa
    37321 agcgatattg tcgaacaccg tcatatggcg gaacagcgcg taatgctgga acacgaaacc
    37381 gactttacga tcacgtgcgt gcaggcggct cacgtcggtg ccgtggaagc gaatatgccc
    37441 gctggtttga tgctccagcc cagcgataat gcgcagcagc gtggttttcc cggaaccgga
    37501 cggccccagc aacgcgacca tctgacctga aggaatatcc agtgagatat cgttcagcac
    37561 ctgggtgcga ccaaacgact tcttaatatt ggcaatctca atgctcatga ttttcctcct
    37621 gctgcgcgcg tttttcctga ttctccaggc gccactgcaa catactcttt aaaaacaggg
    37681 tgataatcgc catcagcgtc aacagcgcgg cggcggtaaa ggagccgact gtgttgtagt
    37741 cctgctccag caattcaatc tgtaacggca gcgaaagtgt ttcgccacga atcgagccgg
    37801 aaaccaccga caccgcgcca aactcgccaa tcgcgcgggc gttggtcagc actacgccat
    37861 aaagcagcgc ccagcggatg ttcggcaatg tgacacggcg gaacatctgc cagccggatg
    37921 cgccaagcaa aatcgccgct tcgtcttcct gactgccctg gcttaacatc accggcacca
    37981 gttcgcgcac cacaaacgga cacgtcacga agatcgtgac cagcaccatt ccgggccagg
    38041 agaacataat ttgcagatta tgttcatcca gccagccgcc gagcgggccg ttggagccgt
    38101 agaacagcag atacaccaga ccggcaacca ccggtgacac ggcaaaagga atgtccagta
    38161 gcgtcagcag taactggcgt ccggggaagt taaagcgcgt caccagccag gccagcagaa
    38221 taccgaacac caggtttacc ggtacggcaa tcagcgcgat catcaccgtc agccagatgg
    38281 cgtgcagcat atccggatcg gccagattct gtaaaaccgg catcagccct ttgctgaacg
    38341 cctgtacgaa gatataaatc atcggcacca gcaggatgaa cgccgatacc agcatcccga
    38401 tgccaatcag aaaccatttg ccccagttaa tcgggcgcgc gtcataacgc ttcaattgtg
    38461 tcacttccgc cattaatgac ctaccacacg ccgaccaaag cgactttgca gagtgttaat
    38521 tgagaacagc agcagcagag atgccgcgag gatcaccgaa gcaatcgcgc tcgccgccgg
    38581 gtaatcaaac tcctgtaagc gaacgaaaat catcagcgac gtcacttccg tcttccaagc
    38641 gatgttcccg gcgataaata tcaccgcgcc aaactcacca agactacggg taaacgacaa
    38701 cgccacgccc gccaccagcg ccggagaaag ttccggcagc accactttgc agaaactctg
    38761 ccagcgcgtt gcgccaagcg tttctgccgc ttcttcatat tccgggccta actcttccag
    38821 caccggctgc acggtacgta ccacaaacgg aatgctggta aaggccattg ccaccgcaat
    38881 ccccagccag gtataggtga ctttgatatc aaacttcgcc agccattcac cgtaaaaacc
    38941 gtttacagaa aagagcgagg ccagcgttaa accggcgact gccgttggca gcgcaaaggg
    39001 taaatccatc agcgcatcaa gcagcgtgcg gcccggaaag cgataacggg ttaaaatcca
    39061 cgccatcagc aggccgaaaa cgccgttaaa aattgatgcc acaaacgccg acagcagcgt
    39121 cactttgtag gccgcgacca cctgcgggtt ggtgatcact tcccagtact gcgcccagct
    39181 catctcagaa agctgcatca ccagcgcgga gagtggcagc agcaatatca ggcacacaaa
    39241 cagcagactg gtgccgaggc ttaaggtaaa gcccggcagg acgcgtcttg atgaaactgc
    39301 aaacattaat tacgccccgc cgctaacagc ttgtctaact cgccgccgct ggtaaagtgg
    39361 gttttcatca cttccggcca ggagccaaat ttgtcttcca cgcggaacag ttcggtctgc
    39421 gggaatttat ctttcagttt gtccatgact tccgggttat tcacgcggta gtaatagtcg
    39481 gtgatgatgg tttgcgcctg cgggctgtaa agccagttca gataggcttt tgctgctttt
    39541 tcggtaccgt tggcttgcac gtttttatcg acccacgcca ccgggaattc cgccagaatg
    39601 ttggttttcg gaatcaccac ttcaaagccc tgcgcttcgt actgtttgcg gatgttgttc
    39661 acttccgatt caaagctaat cagtacatcg cccaggccgc gctcggcaaa ggtggtggtc
    39721 gcgccacgac cgccggtatc gaacacttca acgtttttca ggaactgagt cataaactgt
    39781 tcggttttgg ctttgtcgcc accgtcagct ttatccgctg cgccccatgc cgccagatag
    39841 gtataacgcg cgttacccga cgttttcggg ttcgggaaaa tcagcttcac gtcggagcgc
    39901 accaggtcgt tccagtcgtg gatattcttc gggttgccct tacggaccag gaagcccatg
    39961 gtggagtaga acggcgagct gttattcggc aggcgcgact gccagtccgc cgggataagc
    40021 ttgcctttat cgtgcagaat ttgtacgtcg gtcacctggt tataagtgac aacgtcggct
    40081 tttaagccct gcaaaattgc cagcgcctgt tttgatgacc cggcatgaga ttgttttatc
    40141 gtcagtttgt cgccgccgtt atctttggcc cattgctgtt caaacggcgg gttcagggcg
    40201 gcaaacagct cgcgggagac gtcataagaa ctgttcagca gttccgttgc ctgtacatgg
    40261 cccgccagca gcagagaagc gaccagcgcg agtgagttct ttttcagtaa gttaacggcc
    40321 attgcgcacc cttataaatt taatgacttt ctaatagcca tcatatttat aacggacgtg
    40381 aaaggagtaa cggttttata taccgtttgg tgatttggaa gttgaaaagg gaataagacg
    40441 aaatcctccc cacaaatgca gggaggaaac agaggtgaat cagataccga cgctaaccgt
    40501 ctccggcagt gtgctgccgc catcaatcac attctgtgta ccggttaaat agctggattc
    40561 atccgatgcg aggaaggccg ccagttcgcc gacttccagc ggatcggcga ggcgacgcag
    40621 cgggattgct tttgccattt cagtcagcac cgactctgga tcttccgggt tcgactggcg
    40681 ggcaatgctt tccgccattg gtgtgcgaac gtatccaggg catatggcgt taacgcgaat
    40741 accagactgc gcatactcca ccgccagcga ttttgtcagg ccaacaatcg ccgctttcgt
    40801 taaggcgtac gccgtttcac ccggatcggc caccatatca ccagtgactg aagacatcat
    40861 cacaatgcga ccatctttgc gggcaatcat ctccggcagc accgccttcg tgacgttcca
    40921 tacgccttta atattgatgt caatatggaa atcgcgatcg tcatcgctca tatcgaggaa
    40981 actgcccaga cgacaaacgc ctgcgttatt caccaggata tcaatgcgcc cttctttttc
    41041 cttcgcgcgt ttgatagctg cggctaccga cgccgggtca cgcacatcgg cgacaaccgc
    41101 cgtacagcga tgaccacgac cacacagttc gtccgccagc ttttcgatct caggggagat
    41161 atccagcaag attaggtttg cgccatgacg cgcaaaagtt ctggcaattc cttcgccaat
    41221 tccctgcaat gcgcccgtaa tcagtgctgt cttgcccgtg agtttaccca ttttaatgct
    41281 ctccttgtta atacagagcg ttacactcca ttccctctga aaatacagga taaacaaaat
    41341 attactgcgg atcagctcac taattcagga aaatctcaat ccagtcgctg ctttatgcat
    41401 catttcaggc gttgtgttag ctcactgctg cgctgaatca ttttcaatga ctccacatca
    41461 ttgagttgca caagaccaac aaacagtaaa tcggtaacag aattttgcgc cgtgcgggta
    41521 gacatcgacg agctacgcca ttcggtttca ccggaaacgg tatcgagcgt gaaatgcgcc
    41581 agccgccgca gcggagagtc agccagagaa gtaatggcaa tcaccgttgc cccctgcttt
    41641 cgtgccgctt ctgcgcacaa gacaatctct tttttactgc cgctgtaaga gatagcgatt
    41701 tgtacatcgc cttttttcaa cgcctgggaa acggtggctt gtacgtgcgt gtcggcttcg
    41761 caggccacgc gatatccaat tttcatcagt ttgaacgaca gatcgcaccc caccagcgcc
    41821 gatccgccca ggcccgttat ctggataaac ggtgctttac tgatgacctc aatgattttt
    41881 tgcagccgcg cgtagtcgaa caacgcacag gtttgctcca gcgccagctc tttttcgcga
    41941 ttcaatttgc gggcaatcac ttccagcgaa tcatcactgg taatcgaact gtgcaggtgc
    42001 tgcgccgtgg cattcgtttt ctcccggctg gcactgtatt cgcctattaa cgccatacgc
    42061 aattcagtaa acccttgtgc gccgagcttc tgggcaaact tcacaatact cgactggctg
    42121 atgccgagct gtttcgccat cttgcgcgaa gagacggatt tcagctcact cacgttggcc
    42181 cgcagaaagt cagcaatttt ttgctcgttc tcggtaaatt cactttccgc attgcgtatt
    42241 ttcgtcaggt acaacatctt ccactctcct gggctggcag caaaaattcc gcttaagttc
    42301 ttatgacgct cttcacactc tgcgagtgta gactcaatga ttcctttaag tctgtcaatt
    42361 cgggatatat gattccatac atctgcaatt atttgatcgt aaatagtaag gtcaccaccg
    42421 atgcaacttg aaaagatgat taccgaaggc tcgaacgccg cctcggctga aattgaccgc
    42481 gtatcaacgc tggaaatgtg ccggattatc aacgatgaag ataaaaccgt accgcttgcc
    42541 gttgagcgcg tactgccgga tatcgccgcg gcaatcgatg ttatccacgc ccaggtaagc
    42601 ggcggcggtc gtctgattta tctcggtgcg ggaacatccg gtcgtctggg gattctggat
    42661 gccagcgaat gtccgcccac ctacggcgtg aaaccgggtc tggtagttgg attaattgct
    42721 ggcggcgaat atgccattca gcacgcggtg gaaggcgctg aagatagccg ggaaggcggt
    42781 gttaatgatt tgaaaaacat cggattaacc gcgcaggatg tggtggttgg cattgctgcc
    42841 agcggtcgca cgccgtatgt gattgccgga ctggaatatg cacgccagct cggctgccgt
    42901 acagtgggaa tttcctgtaa tccggggagc gccgtttcca ccaccgctga gtttgctatt
    42961 acgccggtgg ttggggctga agtggtcacc ggttcttcgc ggatgaaagc aggcacggcg
    43021 cagaaactgg tgctcaatat gctttccacc gggctgatga ttaaatccgg caaagtgttc
    43081 ggcaacctga tggtcgatgt ggtcgccacc aacgaaaaac tgcatgtgcg ccaggtcaat
    43141 atcgttaaaa acgccaccgg atgtaatgcc gaacaagcag aagcagcgtt aattgcctgc
    43201 gagcgcaact gtaaaacggc cattgtgatg gtgctgaaaa atctcgatgc cgcagaagct
    43261 aaaaaacgcc tggatcaaca cggcggcttt attcgtcagg ttttagacaa ggaataaccc
    43321 atggccaaag agatcagcag tgaacttctg aacaccattc ttacccgtgt cggcggaccg
    43381 ggaaatatcg ccagttgtgg taactgtatg acgcgcctgc gtctgggtgt acatgacagt
    43441 tcactggttg atcccgatat caaaacgctg gaaggcgtga agggcgtcat tttgaccagt
    43501 gaccaggtgc aggtcgtttt tgggcctggt aaagcgcatc gtgccgcaaa agcgatgagc
    43561 gagctgctgg gagaagcacc ggtacaggat gccgcagaaa tcgccgccca gaacaaacgt
    43621 cagttaaaag ccaaacaaac ctccggcgtg caacaatttc tcgccaaatt cgccaccatc
    43681 ttcacgccgc ttatccccgg ttttattgcc gccggtctgc tgctggggat agcgacgtta
    43741 attgccacgg tgatgcacgt tccggcagat gctcagggca cgctacccga cgcgctgaat
    43801 tttatgaagg tgttcagcaa aggtttgttc accttcctgg tgattctggt gggctataac
    43861 gccgcccagg catttggcgg cacgggcgtc aatggcgcaa ttattgccgc actctttttg
    43921 ctcggttata accccgctgc aaccaccggt tactacgccg gttttcacga tttctttggt
    43981 ctgcccatcg atccgcgcgg caatattatc ggcgtgttga ttgccgcgtg ggcctgcgcc
    44041 cgcatcgaag gcatggtgcg ccgctttatg cccgacgatc tcgacatgct gctgacctcg
    44101 ttaatcaccc tactgatcac cgctacgctg gcctatctga tcattatgcc gctgggcggc
    44161 tggttattcg aaggtatgtc gtggctgttt atgcacctga acagtaatcc gctcggttgt
    44221 gcggttttag ccgggctgtt cctgatcgcc gtggtatttg gcgtgcatca gggctttatt
    44281 cctgtttacc tcgcgttaat ggacagccaa ggattcaaca gcttatttcc catcctttcg
    44341 atggcaggcg cgggccaggt gggcgcggcg ctggcactct actggcgggc gcaaccgcac
    44401 agtgcattac gcagtcaggt acgcggggcg attattcccg gcctgctggg cgttggcgaa
    44461 ccgttgattt atggcgtcac cctgccccgc atgaaaccgt ttattaccgc ctgtttaggc
    44521 ggcgcggcgg gcggtttgtt tatcggattg atagcctggt ggggtctgcc gatgggctta
    44581 aacagcgcct ttgggccgtc tggtctggtg gcgctgccgc tgatgacttc agcacaaggc
    44641 attcttccgg cgatggcagt ctacgctggc gggattctgg tggcatgggt ttgcgggttt
    44701 attttcacca cgctttttgg ctgccgtaac gtcaatctgg actgatatca tgaaacggac
    44761 aatgctctat ctttctctgc tggctgttag ttgtagcgtc agcgccgcaa aataccctgt
    44821 tctgacagaa agttcgccag agaaagcagg gttcaacgtc gaacggctta accagatgga
    44881 tcgctggatt agccagcaag tggacgccgg ttatcccggc gtaaacctgc tgattataaa
    44941 agataatcag attgtgtatc gcaaagcctg gggagcggcg aaaaagtacg atggcagcgt
    45001 attgatggcg cagcctgtaa aagctaccac cgggacgctg tatgacctgg cctcaaacac
    45061 caaaatgtat gccactaact tcgctctgca aaagctaatg agcgaaggca aactgcatcc
    45121 tgacgatctg attgcgaaat atatttcggg gtttgccgat agtccaaatg acaccatcaa
    45181 aggcaaaaac acgctgcgga tttctgacct gctgcatcac agcggcggtt tcccggcgga
    45241 cccacaatat ccgaataaag cggtcgcggg cgcgttatat tcccaggata aaggccagac
    45301 gctggagatg atcaagcgca cgccgctaga gtatcagccc ggcagcaagc atatttacag
    45361 tgatgtcgat tatatgctgc ttggatttat cgtcgagtcg gtcaccggtc agccgcttga
    45421 ccgctatgtt gaagagtcga tttatcgccc gctcggcctg acacatacgg tatttaaccc
    45481 gctgctgaaa ggctttaaac cgcaacaaat tgccgccacg gaattgaacg gcaatacccg
    45541 cgatggagtg atccacttcc cgaatatccg cacctccact cactggggtc aggtgcacga
    45601 tgaaaaagcc ttttattcga tgggcggcgt ttccgggcac gcaggtttgt tttccaatac
    45661 cggcgatatt gcggtgttaa tgcaaacgat gctgaacggc gggggctatg gcgatgtgca
    45721 gctgttcagt gcggaaacgg tgaagatgtt caccaccagc tctaaggaag atgccacttt
    45781 tggcctcggc tggcgcgtga atggtaatgc caccatgacg ccgacgtttg gcacgctggc
    45841 aagcccgcaa acctacggtc acactggctg gaccggaacg gtgaccgtta tcgatccggt
    45901 gaatcatatg gcgattgtga tgttaagcaa caagccacat tcgccggttg ccgatccgca
    45961 aaagaatccc aatatgttcg aaagcggtca gctgccgatt gcaacgtatg gttgggtagt
    46021 tgatcaggtg tatgcggcgt taaagcaaaa gtaataaaaa ggccagtcag taatgttact
    46081 gcctggcctt taatcaatga atcagaaacg cttacagcgc catcaacttg tccagcgacg
    46141 gtgcgaaata atagccgccg gttaccggtt tggtgaaacg caacatcgca tcacgcttac
    46201 catcggtatc gccaaacatg ctcagcagtt gctgctcaat gttatgcaga cgcgcgcagt
    46261 aggcgcagaa gtacagaccg tgagtgccac tggcggtgcc gtacggcagg ctctggcgaa
    46321 caatcttcag ccctttgcca tcttctttca gatcaacgcg ggtcaggtga gaggtttccg
    46381 gacgttcgtc gccgtcaatc tcttcgttgg cctctttggt gcgcccgatc atcatctcct
    46441 gatcgtgaac gctcatccgg ttgagctgct tcaggttgtg ctcccaacgc tgcacaaaca
    46501 catagctgcc gccggcatcc acgccgtctt tgataaccgc cacttcgcga cgcgtctctt
    46561 cacccgccgg gttttctgtg ccgtcaacaa agccgctcag gtcacgctct tccacccaac
    46621 ggaagccgtg gatctcttct ttcacttcaa tgcagtcacc aaaggcttcc atcgccgcct
    46681 gggcgacaga gaagtttacg tcgtgacgca gagaaagaat gtggatcaac acatcgaact
    46741 gggtggtcgg cgcaaggcct ttaccgtagc ccggaaaatc tttcagctct tctgccccaa
    46801 cgccgccgct cagagcgcgc cagatgttgt taccaaaggc aaccaccgca ccaagatgcg
    46861 cgtccgggaa tttcgcttca aaagttgcca gtttgtcggc aaatgtttta ctggccgcac
    46921 gcagggcgtc aacttcccct ttcacgttgg cttcgatcca aatcgccgcg cggcaatgtt
    46981 ctggcaaaat gccactctga acctgagaca ttgttcctcc tgaaaataat aatgccacgc
    47041 aatccgtggc attgttgagc gctattttac gtgttttttc gcggcggcgg tttgttcaga
    47101 cgcaaattaa cgccgccaga taattttact gactttccag tttttcaggg tatcgtccga
    47161 aggcattaac ccttcaggac cactccattc cccgctgaac tggtagctaa tatgttgact
    47221 gccttcggct ttacattcga cggcacgatt atcatcacca tcagcttttt ggcaattgcc
    47281 aaatgctttg ctgtaaaggt cgctaaacgg tgtaccgatt ttaacaccgg tgtcagcagg
    47341 aatatcgcta tccagcacgt caatgcgact gatcgtgcct tgatcgccgt taatcaccat
    47401 cgccacgttg tcgcccttca tcacttcaaa aaagcgcacc acgttgccgt tcgcggtttt
    47461 cattccgctg cgcaggcgat aatcgccatc aaccgcatcg gcaatggctt gttcttgcag
    47521 tggtgtggac gccgttaatt caccaacgcc ctgctcgctc actttggtgg acgacccaaa
    47581 ccagttccac gggttagcgg cagaccagtt aactgacgac atcgtggaac agccggtcag
    47641 catcaatggc atagcgcata acattaaacg cagcgatttc atgtcacttc ctttggttat
    47701 tcaataacgt tgcttggagt gcaaattcac caaaaagtgc cgttattctt ttacttcatt
    47761 aaaacaagcg cgtaaacgtc gattggtcag cagccagatt aacgccacaa tatccgccac
    47821 taccagcgcc agaccgatac cgctaacgga ttcaccgttc agccacagcc acggttgcca
    47881 gcaaagtaaa accacctgcg ccaacagcaa cagaaaatag agtacacgcc aggtgcgagg
    47941 aaacgtagcc cgccgaccgc tcaacaaaaa cgccagcacc gccggaatgc caggaatcag
    48001 ccccagccag aaattatcgt gatcgggata aaacagattt agcagcgcag taccctgctc
    48061 gcgcgacgca ccggcaatga caaacagcac ccaggttcgc gcctgaagca atagcacaag
    48121 ccagaagagc aagggtaaac gcaggcgacc gtgcgcatca taatggacag gatgaaactc
    48181 agtactcttc atcttcaatc aaacgcttac ccagactcag cacgtcggcg tgttcatatc
    48241 ccaggcgttc atacattccg agcaccatgt cgttatcttc cggcacgttg atctgaattt
    48301 tcgggcagcc gcgagcaatc agctttttct ccagccggtt aagcaacgca ttggcaatcc
    48361 cacgcccacg aaactctgga tgcacgccaa gataataagc agacccgcga tgcccgtcat
    48421 aaccgcccat caccgttccg accacttcac cgttcacctc agcgaccaga aacaaactga
    48481 cgtcatggtt catcttacgc tcgatgtcca tttccggatc gttccacgga cgcagcaagt
    48541 cgcaacgctc ccaaagggtg atgacctctt cgaaatcttc ctggcgaaat acgcgtatct
    48601 ccatggtatt cgttaccttt ttgcgggtta aaaggctgat tatggcgtga acggtcgaat
    48661 tagccaatat ctgacgaaaa tcggttgaaa aagtggcata atggggagtt gtcaactatt
    48721 gaaatgaaaa gtaaaacaat tctcaacagc aaacggtcgt aacggattac gcgatacgat
    48781 ataacatctg gaactttatt attacaactc aggccgtatg agcactttta aaccactaaa
    48841 aacactcact tcgcgccgcc aggtgctgaa agccggtttg gctgccctga cgttgtcagg
    48901 aatgtcgcaa gccatcgcca aagacgaacc tttaaaaacc agcaacggac acagcaagcc
    48961 gaaagctaaa aaatctggcg gcaaacgtgt cgttgttctc gatccaggcc acggcgggat
    49021 tgataccgga gcgatcgggc gcaacggttc gaaagaaaaa catgtggtgc tggcgattgc
    49081 taaaaacgtc cgttccattt tgcgtaatca tgggattgat gcgcgtttaa cgcgttctgg
    49141 cgatacgttt atcccacttt acgatcgcgt tgaaatcgcc cataaacatg gcgcagatct
    49201 gtttatgtca attcatgccg atggctttac caacccgaaa gctgccggtg cttcggtatt
    49261 tgccctctct aaccgtgggg caagtagcgc aatggcgaaa tacctgtctg aacgcgaaaa
    49321 ccgcgccgat gaagttgccg gtaaaaaggc gactgacaag gatcatctat tgcagcaagt
    49381 gctgtttgat ctggtgcaaa ccgatactat taaaaacagc ctgacgctcg gctcgcatat
    49441 tctgaaaaag attaagccgg tgcataaact gcacagccgc aacaccgaac aagccgcgtt
    49501 tgtggtgttg aaatcgccgt cggttccttc ggtgctggtg gaaacctcgt ttatcaccaa
    49561 cccggaagaa gagcgactgt taggcacggc ggcgtttcgt cagaaaatcg ccacagcgat
    49621 tgctgaaggc gtgatcagtt atttccactg gttcgacaac cagaaagcac attcgaaaaa
    49681 gcgataagtt atgaaacccg acgcacacca ggttaaacag tttctgctca accttcagga
    49741 tacgatttgt cagcagctga ccgccgtcga tggcgcagaa tttgtcgaag atagctggca
    49801 gcgcgaaggt ggcggcggcg ggcgtagtcg ggtgttgcgt aatggtggtg ttttcgaaca
    49861 ggcaggcgtc aacttttctc acgtccacgg cgaggcgatg cctgcttccg ccaccgctca
    49921 tcgcccggaa cttgccgggc gcagtttcga ggcgatgggc gtttctctgg tagtgcatcc
    49981 gcataatccg tatgttccca ccagccacgc gaatgtgcgc ttttttattg ccgaaaaacc
    50041 gggtgcagag ccagtctggt ggtttggcgg cggcttcgat ttaacccctt tctatggttt
    50101 tgaagaagac gccattcact ggcaccgcac cgcccgtgac ctgtgcctgc catttggtaa
    50161 agacgtttat ccccgttaca aaaagtggtg cgacgattac ttctacctca aacatcgcaa
    50221 cgaacagcgc ggtattggcg ggctgttctt tgatgatcta aacacgccag atttcgacca
    50281 ctgttttgcc tttatgcagg cggtaggcaa aggctacacc gacgcttatt taccaattgt
    50341 agagcgacgt aaagcgatgg cctacggcga gcgcgagcgc aattttcagc tctaccgtcg
    50401 cggtcgttat gtcgagttca atctggtctg ggatcgcggc actctgtttg gcctgcaaac
    50461 tggcgggcgc accgagtcga tcctgatgtc aatgccgcca ctggtacgct gggagtatga
    50521 ttatcagcca aaagatggca gcccagaagc ggcgttaagt gagtttatta aggtcaggga
    50581 ttgggtgtaa ctccctcacc cccattcccg catccgctga tgcaacgtca acgacggctt
    50641 ctcggcaaac agctgctggt aatccgtggc aaattgcccc agatgccaga atccccactg
    50701 catggcggcg tcttttaccg ttgtgctttg cgaccacggg cttatcagtt cgcggcgtac
    50761 ggcgttcagg cgaatgcgtt tcagccacgc gttcgggcca attcctaaaa tagcgtgaaa
    50821 cgcgttttgt agcgtgcggc ggctgacatg cagttgatta cacaagtcca gcaccgtcac
    50881 cggttcggac atgttttcca gcacatattc acgggcgcgg gaaagcaacc gacggtaact
    50941 ctgatgactg atgctttccg ccgtcaccat tggctgcgct tcttccagca tcgcccccat
    51001 cgccattagc aaattatccc ccagcacttt tcgcactgca ggctgatgga gattttccgg
    51061 gttctcgcaa aacgtcgcca gcgcctgttg gacaaagccc cacagcgcgg ctttatgctg
    51121 ctctttcact tccagcgccg actggctacg caacatatgt aatacccgat ccggattatg
    51181 caaaaagtta gcctgacggg tgatgacatc ttcagaaagc actacgccca ggatcgtgta
    51241 atcgtccggc gtacttaatt caaactcggt tccaccaggg cgcgtcgcta tttctgcgct
    51301 tcccagacat tgcgaaccga taaatccctg ctcaccgcgc gtcgccggaa tgccaaacca
    51361 gaacgagttc ggccagacca gacacgactg acgcagcgcc agaccggtgt attcacgaaa
    51421 aacctgaata tcatcgagta gaatttccgt aaattcacca tgaaacttgc ccggatgcag
    51481 ctgatcgtaa atctgctgcc aggcggtaat cgttaaagca tgttcataga catccgttgt
    51541 ctgtcgttga tgaacattat ccacttcgac cttcggcgtg agcttcaggt tttcgggtaa
    51601 gggttcatga taaagatggt gcaaattggc tgtacgggtc tttttcatga tgttaatgcc
    51661 gggtgttgta ggacacccgg cacctccgac aggttaatgg ggcttgagac gataacgact
    51721 actgcgttta cgtaacgtcc cagcagaaaa gagctgttcg agcgcatttc tggctttttc
    51781 cagcggccag ccaaagtggg cagccacttc tcctgccgtc actccctgac gtactgatgt
    51841 taacagcgcc aacaactcgt cagcagattc cgcgacgata actggcgcgt cgggttcctt
    51901 tacgggttgc ttcggctggc ggttaaagcc agtaaccagc cactgggtgt catcgtctgg
    51961 ccgaccgatc tccttacggc tgatgacacg cccggtacgc atcgcggcag cacaacctgc
    52021 gtccagcgca gcacgacacg ccgccagatc gccttccacc accagcgtta agcgacctgg
    52081 gtcaagcact tcgtgactga gcagacgaac gttagctgct ttgagcatgg catccgctgc
    52141 atctatcgcg gcgaccatgc cgtccacttc cagcaatccc agtgcattga tcattggcaa
    52201 cctccgttac gcacgctgga ttggattacg cgcgatttcc agcactgcat cggtaaaggc
    52261 gttacaggcc gctttacacg ctgcctggct accggttaaa aatgctgccg agtagttagt
    52321 ttcagacggt ggcgggacat aggtcaccag ctgtacgtcg gcagatttca acgctgcatc
    52381 aatgccgtag gtcgcttcca gcggcggtgc caccagatac gccatcggat cgccaagcgt
    52441 gatcccggcg gttgatgaga gataagaacc ggtacgcgaa actacatgtg ccaggaatgc
    52501 cgtatcttgc gcatcattag cccactggaa agcagcgcca ttttcaatat gcgcaaccat
    52561 cgcatccaga ccggcacgca cttccgccgg gtttggccca ccaagcataa tcagcacctc
    52621 accggcagtc ggtgacgggc cgtgtgccgc gcccgcatac agcgagcgac catataccac
    52681 ttctaccatc gcttgtttgg tcgcttcgtc agccgcaatg taggttacgt cgtcagaatc
    52741 ggcagaaatc agaccgaggc tacgaatatg tggcggcaat ttcagttcac gcgcaaaatc
    52801 ggcgttaacg gaggcaatca cccgcatggc ggttaccgac ggtcgaatca aatctaaagc
    52861 tggcatgatg cctccttatc gggtcatgtt gatgccggat gctttctgct ccagcatacg
    52921 tttcgccaaa tccacaatga cggctgcggc ttcaaccggc ggcgtgccgc cctggtgaat
    52981 gttagagata caggtgcgat cggcctcaac ggtggtcgcc atacgcggcg agtaaacggc
    53041 gtagcaggag aggctttctg actgacccaa ccccggacgt tcgcccacca gcaggatcac
    53101 tactttcgcg ccgaggatct caccgatctg atcttcaatc ttcacgcgac cataacgaac
    53161 aaagaatggc gtgccgactt tcagcccggc ctgtttcagg cccgccatca gcggcggcag
    53221 gatctcttcg tagttcacag tgatggcatc agttgacagg ccatcagaaa taacgacctg
    53281 tacgtccgga ttagcaacac actgtgcttt cagcgcttca acagcttctg cacacaggcg
    53341 gcggcccata tccgggcgag tcaggtagag atttttgtcg ctaatttcgg agcgtacttc
    53401 cagcaagccc tgcgctttca cccactcttc cggcacttct ttcagtacgg tatctttcga
    53461 acgagagtga tcggccagga aacgcagcag cgccagcgta cgcggacgcg gaccggcacg
    53521 accggtacaa acgcgggcca cggtgctgcg acgcagttct gttaacacat ccgcacgatg
    53581 tgggttttca acaccaatcc acacttttgc ttccgcagaa cctaaatcca gcgcgcagct
    53641 ttccgaggtc accggtgccg cacaggtggt agtggcgcac tttgcttctg acggcgcggg
    53701 ggccgtttgt cccattgacg ccatcacgct gcgtacaatt tcttcaatct gtttttgatc
    53761 catgatgtgt tatctccgcg tcatcagaag aacagtgacg gatcgcccgc ccgtttggtc
    53821 aggcgaccgt ttgccataat gcccatgctt tccagccagc gttcaaactc cggtgacggg
    53881 cgcaggttga gtaactgacg cacagtggcg gtatcgtgga atgcggtggt ctggtagttg
    53941 agcatgatgt catcacccag cggcatcccc atgatgtagt tgcagcctgc ggtggcgagc
    54001 aggatcatca ggttttcgtt gaggttctgg tcagcgtcag cgtggttggt gtaacagcag
    54061 tcacagccca tagagatgcc gctcagcttg cccataaagt gatcttctaa gcccgcacgg
    54121 ataatctggc ggtcgttgta gagatactcc ggcccaataa agccgaccac ggtgttgacg
    54181 ataaacggat cgtaatgacg cgccagccca tagttacgtg cttccatcgt cacctggtct
    54241 gcgccgaagt tagcgccagc ggatagcgct gaaccttgtc cggtttcgaa gtagaggcag
    54301 ttttccccgg cgatacggtt gaactctgca cccactgcgc gcgcttcgtc gagcatcgcc
    54361 agttccacgc caaactcttt cagccctttt tcgctgccac agatactctg gaaaatcagt
    54421 ccgccaggcg cgccgcgacg aatcgcttcg atctgggtgg tgacgtgcgc cagtacgcag
    54481 ccctgagttg ggatgttgaa tttgtcgatc acgccataga tggtgtccag cacgcggctt
    54541 aagttttcca cgtcgtcagt caccgggtta acgccgatca ccgcatcgcc caccccgaag
    54601 gaaagccctt cgtagatttg cgcggcgata ctttgcacgt cgtcacgggt gtcatttggc
    54661 tgcaaacggg cgctgaaggt gcccggaata ccgatggtgg tattggcctt tttgattacc
    54721 ggcattttct tcgcgccgta gatcaggtcc gcgttggagc aaatcttcgc taccgccgcg
    54781 accacttccg aggtcagccc tttgcgggta aaggcaatgt cgtccacgct ggtttcatcg
    54841 ctcagcacat actcacgcag ttcgctgatg ctccagtttt taatctggtt gtaggccgtt
    54901 tcgttaacat cgtcctgaat cagccgcgtc acgcagtcat cttcataggc aatcaccgga
    54961 ttattgcgga tgtccgctac ggtcatttcc gacaacacct gctttgccgc cacgcgctcc
    55021 tgtgagcttg ccgctgcgac gcccgccagc acatcccccg aacgcagttc gttggcttta
    55081 gccagcacct cttttacatc cttaaactga tatacattgc cgaacaatgt ggtctttagt
    55141 ttcataagtc gttccctcag gaaggaaatg cgagtgattt caccgtcacc ggcacaaccg
    55201 atccgccaaa aagaggcgta ccaatgtcga tatagtcccc cgcgcggaca atcacttcgt
    55261 caatgactgc caacgggagt tgttgtagct gtgggcgcaa cagcatgccc agagctttac
    55321 caaagtcctg cccggccacc accagcaggg gatgcggatt cggaaaacgc gcgacgaaat
    55381 cgaccaacgc gttgatgacc gtcagtaccg cggcgtaacg cacaggcagc gaggcgggaa
    55441 gcgccagcac gtacgcgtca gttttgggat caagatccag ctgaatcagc gcctgttgcc
    55501 aggcactcac cagatccgtt tcatcaatcg ggatcgccac cggcaaattg cgcagcggca
    55561 gttgtacgcc ctccagccag attgtgctgc cagagagcga aagggtatgt gcgcccgcgc
    55621 caatcaccgt ggcgcgcacg gtttgcgccg gaaactgcac gttcatctca cgcaggcgcg
    55681 gatggtcatg tagcgccgtc gccagtagcg ggccaatatc ggcaaaacag aacgggtcag
    55741 cgggctggtg gcgataacat tcgcccacgc cgccagaaag cgtaatgatt tcgggcgtaa
    55801 cacctgcggg cagcaaaccg gtttgcatta atgcctgcgc gagcggcgaa agcgttccgt
    55861 caatcacttc gacaatcagc tctgccatcc gtcgggtaac ctgcaccagc tgcgcgccgg
    55921 tcagcgaacg gacgtcagtg cctgcaccga aacactcatc cacaatcatc tgccccggtt
    55981 tatgagcgta aaccacgcgc ccctggctgt cggtttccag caggcgacca ccgacgttga
    56041 ggcaggcagt gccgctgatt tttccggcat cgaacagggc gtagttcgcg gtgccaccgc
    56101 cgatgtcgat attcagtacc cgacacagcc gttgttcaga aagggtttgc gccccggcac
    56161 cgtgaccggc gatcacggat tcgaggtgcg gcccggcgct ggcgacgaca aaatcgccca
    56221 gcgactgaga gagcgccatc accgccgggc gagcattgcg ggttttcgcg ctttcaccgg
    56281 tgatgatgat ggcaccagaa tcaacgcttt ccggcgcaat gcccgcagcc tgatattgct
    56341 ctaaaatcag cgatttcagt tccgcttctt ttaaaccacc ctgtttatcg acaggggtaa
    56401 agaacaccgg gctttgccag ctaatttcgc gtttgatgaa ttcgtagcgc ggcacctgcg
    56461 acaccgccgc acggttaacc agttccaggt gggagaaaat cacctgggtg gtggtggtgc
    56521 cgatatcgat accgacgctc agtagctggc gagtgttcac gattgtgcct ccgcttcggt
    56581 tttagccgcg gtcgcgtcgt cttttggcac cagcatcatc gccacgccaa tcgccgttac
    56641 gccgccgatc aacttgccga caatcatcgg gaagatcatg gcattcatgt tggcagcggc
    56701 gaagcctaag tggtcgccca gggcgaaagc agcggaaacg gcgaaggcgc agttgatgac
    56761 tttgccgcgg gtatccatct gcttcatcat gccgaacatc gggatgttgt tggcaagcgt
    56821 tgccaccatg ccggctgccg cgatgttgtt catattcagc actttaccga cgctcatcag
    56881 cggtttttca aaccagcggg tcagcagcag caccatcgga tacgccccta acagaacgca
    56941 ggagatagaa ccgataactt caatggcgcg catcacctca ccgggtttat cgccaggggc
    57001 cataaagata ggatccagac cggggatcag ttcccagcca agcaggaatt tcactaccgc
    57061 agcggcaaga ccgagggtga tcaatgcaac gaggaatttg gcgaagatct ggaagccgtt
    57121 gatcattttt tctgggatga atttcagccc cagcgccacc agaatcgcaa caatgatcac
    57181 cgggatcatg ttcatcagga tcagggcgaa agtgaattcc accggctggc cgttgatctg
    57241 aacaccggag tacatagcaa ccagaccacc agcgatacaa ccaatcggaa tggtcacaat
    57301 gcccgccagc acgccgagcg ccagataacg acggtcagaa ggttcgataa tgccgagcgc
    57361 caccggaatg gaaaacacaa tcgttggccc catcatcgac ccgagaatta acccagagta
    57421 tagccacgcg gctacgtcgc cgcccgccag ctctttggcg aggaagaagc cgcccatatc
    57481 gcacgccagc agtgttccgg cgaacatcga tgggttagcg ccgagcattt cgtaaaccgg
    57541 aataattacc ggcccgagta cgtgagccag taccggtgcc agcgcggtca taccgaccat
    57601 cgccaggccc agtgcgccca ttgccataaa gccttcttcg aactgaccgc cggatccttc
    57661 gatactttta ccgaacttac cgaggaaacg agcagaaccg ccgaactgtg acaggatcct
    57721 gtcaacggca gctatcagca taaagaacat catgatgtac atgatgattt cgttaattcc
    57781 catcgccttt actccctgtt agttgttatt tattagcgga tgctgcgtga agccttatcc
    57841 gcactacatg tgcaatccca cagaccggat aagacgcggc atttattgcg ccgctgcgta
    57901 caggccgaca agctgttcca ggctggcggt acgcgggttg ctgcgcagac aaatatcttc
    57961 cagcgcggcc tgcgcccatg caccgtaatg cgcagatgtc gcgccaacat cgcccagtcg
    58021 tttaccaatc ccaacttccg caatcagctc acttaccgcg ttaatagcgt cacgatcgtc
    58081 ggatttttta gttcgcagtg cccgaccaat ctgactaaag cgttcgcgac aaaccatccg
    58141 gttaaactcc atcaccgttg gcagcaacat agcgttcgcg agaccgtgcg gaatatgcag
    58201 cgccgcgccc ggctgatgcg ccatcgcgtg gcacaaccca agacccgcac tggaaaacgc
    58261 cattcccgcc atacatgaag ccagcaacat gctctcgcgc gcggcaaggt cgtgaccgta
    58321 gcccaccgct ttcggcagcg atttgccaat catcgcaatg gcaccaatcg ccagactgtc
    58381 ggtaaacggt gtagcgttca gggcgctgta tgcttcaatg gcatgggtta acgcatcaat
    58441 gccggtcatc gccgtgacat gcgacggcac accttcggtc aatgcggcgt cgaggatcgc
    58501 cacatccggc atcagcgagg catgggctaa cacctgcttg cgcccgctca ccgcgtcgat
    58561 aatcaccgtt acattggtgg tttcagagcc ggttccggcg gtagttggaa tggcaatcag
    58621 cggcaagcgc ggttgcagaa cgctggtttc tgacatctct gccagcgtgc tatccgggtt
    58681 cgtcaccagc aacgccacgg ctttcgccgc atccagcacc gagccgccgc caaatgcgat
    58741 caccccgtca caacctgact cacgcaactg cgccacggct gcacacacgt cggtaatgca
    58801 cggttcgccc accggacatg gccagagcgt catggcgata cctttaacgg ccaggctacg
    58861 cgtcagcccg gcggtcatcc ctgcctgatg caaaaagctg tctgccatca cgaacagatg
    58921 tttcagccca cgcgtttgcg cttgctgtcc gcaactgctc accgagcccg gaccgcaaag
    58981 cgtcaccggt ggaacgctaa atgtttttac ccgttgcaga ttcagggtat cgaacgcctg
    59041 aaagagcgcg gtctgcaatt cattttgcat atagcccctc cgctttctct cgcccactac
    59101 tggcgatcgc cagcggggtc ataaacaaac tgtgctgcgg taaatgcacc tgtaacgccg
    59161 ggaattgttt gcgaaacagt tctgccacgc ccggttgcat acaggagccg cccgccagcc
    59221 ataaatcagt aatgccctgc ccttcaatat gccgggcgac aatgtccgcc attttttcgt
    59281 acaccggttt caccgtaggc caaatctctt caccgtgacc gcgcttgtac tgctccgcct
    59341 cttccagcga aatacggcga tttccggcga aagtcagaga gatgtgatgt ccgccggtgg
    59401 cttcatccgc cgagtacgtc actttgccct ttttcacgat ggcaatgccg gtagtgccac
    59461 cgccgatatc cactacaccg gcgttgtcca gttgcagcag atccgcgact gcggttggct
    59521 catccagcac atggctcact tccagcccgg cagactccag cacgttgatg gaaatacgcg
    59581 ggtccgtacc tggaggaaat gaagtcgccg catggctaaa acgacgaccg aattgctgct
    59641 cgagcgtgtc gagatggcga cgaacaatgg tgaccgcgcc gaagaaatcc cagacgatgc
    59701 cgtcgcgaac cacatcggcc cagtcgaggc ataccgccac tggctgaccg tcgcggtcga
    59761 caaccatcga caccacatcg caggtgccca gatccactcc cagccacagc ggagattccg
    59821 tcgcggcggg cgtctggtta cacagcgttg ccgccgtttg cagacgtggg gtgagccatt
    59881 gttcgtcgtg cgccatctgt tactccttaa acaatgcgaa acgcatcgac taatacacag
    59941 cgacgcagac ggacaaacgt acgcgcgctg gtcacccctt caccggttgg cgtggtgatg
    60001 gtcatggtgg tccagccttc cccgcccagc cccagcccgg caatgcacgg tccgttctta
    60061 acgaaaatgc tggtatcaat agcattcgcc atctggttca tgttttcgat gttgcgcgag
    60121 tgcattgccg ccgtgtggtg gcaaccgcct tccagtttca ccgctagcgc aatggcatcc
    60181 gccacgttgg cgacgcgcac gacgggcaac accggcatca tcagttcagt cacggcaaac
    60241 ggatgttctg cggtggtttc cacaaacagc aggcgcgttt cttgcggaac tttaaggccg
    60301 attgccgccg cgattttgcc tgcgtcgcga ccaacccagt cacggctgac ggtgcctttt
    60361 ccgcgctcgt cgatattttt cagcaacacc ggttgcagct gctgcgcctg ttctgcggtc
    60421 agtttcaccg cgtgctggcc ttccatcaga cgcatcagtt catcggctac gctatcaaca
    60481 acaatcagta ccttttcgtc ggcacaaatg atgttgttat cgaaagacgc gcctttgacg
    60541 atggactgag cagcacgggc gaggtcagcg gtttcatcca ccactaccgg cgggttgcca
    60601 gcgcctgcgg caatcagacg tttattggtg tgtttacgcg ccgcttctac taccgcttcg
    60661 ccgccggtca ccaccagcag gccgataccc ggaaacttga acaagcgttg cgcggtttcg
    60721 atatccggat ttgccacagt aaccagtaag ttttccggcc cgcctgcggc aacaatcgcc
    60781 tggttgagca gcgtgatcgc ccgctgggag acttttttcg ccgccggatg cggggcaaaa
    60841 atgacgctgt tgcccgcggc aatcaggctg atggcgttat taattacggt tgccgccggg
    60901 ttagtggacg gcgtcaccga agccaccacg ccccagggcg cgttttcaat taaagtcagg
    60961 ccgttgtcgc ccgtcagcac ttgcggagag agacactcaa cgcctggcgt gccgcgtgcc
    61021 tgagcgacgt ttttggcaaa tttatcttca acgcgaccca tgccggtttc actgacggca
    61081 agttccgcta aatctctggc gtgtttttcg cctgcttcac gaatggcagc aatggctaac
    61141 tggcgcattg ccacgctctt taacccttgc tgggcaattt tggctgccgc aacagcgtca
    61201 tccagggacg cgaaaacgcc catctcatga acggcggcgg gcggcgtgtc actgctttgc
    61261 attttcagca gtaccgcttt caccacctgt tcaatatcct gttgattcat gatgttctgc
    61321 cttatttgtg gaaaattacc tgaccgccag acaccacctc atcgacgatg ccaatcacgc
    61381 acagatcgac cggtgacgtt tcgcttttat gcgcctggcg ggcggaactg ccactcacca
    61441 gcaacaccca ctccccggtt cccgcgccaa tattgtcgat ggcgacggca cattgcccgt
    61501 caggattacc atgtggatca atcatttcca ccatcagcaa tttgtcatgc gccagtccgt
    61561 gatggcgtac ggtacaaaca atttgtccag tgacgactgc cagtttcata cccgcctccg
    61621 tggcgtattt caggtaaaag ctccccctac cctccgcgga aggtaaaatg aaaaatgaga
    61681 gagcgtgacg cccgaatcga cgtcacacag ggtgattaca ggttgctgct atcgcctttc
    61741 aggccgatcg ggaacacttc ttccaggtcg ccgtgcggac gcgggataac gtgtacagat
    61801 accagctcgc cgatacgctg tgccgctgcg gcaccggcat cggtcgccgc tttacaggct
    61861 gcaacatcac cacgaaccat ggcagtacac aggccgccgc caatctgctt aacaccaacc
    61921 agcttgacgc gcgcagcttt caccatcgcg tcagaagcct caatcagtgc aaccaggccc
    61981 cgggtttcga tcattcctaa tgcttccatt gtgctttcct ctttatcagg gtccagaacg
    62041 ggaccgttca ttcaaccagt gtttgtaaac tgctttcgcg gttcacttct gtctgacgcg
    62101 gcacagctgc caccagcgcc agctcgataa tttcctgcac actacaacca cgagagagat
    62161 cgtgcatcgg cgcggcaagt ccttgtatca atggcccgac ggcacgatat ccgccgagtc
    62221 gttgtgcgat tttgtagcca atatttccgg cttccagcga cggaaaaacc atcacattgg
    62281 ccttgccctg tagcgggctg gcaggcgctt tttgcgccgc cacttccggc acgaaggcgg
    62341 cgtcaaactg caactcgcca tcgactacca gctttggtgc gcgctcacgg acgatttctg
    62401 tcgcctgctg gacattggca acgcaggggt gacgggcgct accgttactg gaaaacgaca
    62461 gcatcgccac gcgcggctct tctccggtga tggcgcgcca ggtttcggcg ctggcaagcg
    62521 cgatatccgc cagctgcgcc gccgtcggct gtggtaccac gctgcaatcg gcaaagccca
    62581 acgccggacc gctgtactgt ggcagcatca ggaaaatgga tgagagcgtt ttacagcctg
    62641 gctgcaagcc gataatgcgt aatccggcac gcagcacatt cgccgtggaa gagaggttgc
    62701 ccgcgataca gacatccgct ttaccggcgc tgaccattgc ggcggcgaac atcagcggat
    62761 cggtaagttt ttccagcgca tccggcggcg ttttttcgcc cgcgcgggcc agccagcgat
    62821 gagcaaattc ttcccgcatt gcgaggttgc catgcggatc tatcacctgt agcccgtcca
    62881 tcgccacgcc gtgactgagc gcaaactgac gaagttcaaa cggattggcg accagaatgg
    62941 gcgttgccag accttgttga tgtaaatatt gcgcagcttt cagcacacgt tgatctaacg
    63001 catccggaaa aaccactctg gcgggcgctc gcaacgccag ttcacaacaa cgttcaataa
    63061 tcattgcgtt tcccccagtc gttgttggat ctgcttcacc gttagcggct gcttcgttac
    63121 gctcaggatc atcatcacgt agaccgtgct ggaaaggcgg ttcagcgcct gcaagatgtc
    63181 cgggcgtaat acttcaaaac tgcgggtgat aaacacctgc gccgctacgg tttcggcttc
    63241 ccgcactttg gtgcgtagca gattcagcaa cgccgcatcg cgcccgtggc ttgcttccgg
    63301 caccagatga tcgtgaccaa gataacgcaa cggctgatga gaaagccggt gcagatcttc
    63361 gtcagaaagc ccgacaatcg cctggcagcc cagcggttcc gccagttcaa tttgcagcca
    63421 cactgccagc gcaatggtgc tgtcgagaac tgcgcgaaaa cccagacgcg gatcgctttt
    63481 ggcgaccatt ttttccgccg acaggtgagt cagcgtatcc ggcttttttg ccaccggctg
    63541 gcggcacagt tcgcagcacg cctgcggatg ttcatcgcta ctggttagcc catgaacggg
    63601 ctgcggctgc tgctgttcat cgtcaacaaa caggcggccc tgctcgtcaa taaacttgat
    63661 gcgcagatgg cggctttcca gcaactcccg ggcagagggc gtcaggcgac tgtccgcagg
    63721 gagatggatc tctgcgcctt cgctgagcgt atggttcgct cttagccatg cttcggtgat
    63781 gaaatctttc atagggattg ccagttagcc ggccaggcga catacaggaa tttcacgctg
    63841 gatgtcgtac caaattcgat gctggagcct ttcgggataa acatcacgtc gcctgcttta
    63901 gcgatcatgg tttcgccttc gtggcgaaca tggagctccc cttccagcac catgtcgatt
    63961 tcgtcgtagt tcagggtcca cgggaagaat gcgttctccc actgcataaa accggcggcc
    64021 atgctgctgc cgtcgtctcc ggtcacaaga tcagtcaagc ccacgcaatg cggttgtgcg
    64081 ccatcaaagc gaccaaactt gacgctgcta ccatcaatca ctttgatgcc gcctttgccg
    64141 gtcaccgatt tgaagctcgg ctgcattgca ccctgctcca gcgactgttt ttccttcatc
    64201 actttttcca tcagctgcgc gaccaggctt tcggtaaact ggccttccgg tagctgggcg
    64261 ataatggttt cgcggatgcg ctggctttcg gttttatcgg ctggcacgct ggcgggcacg
    64321 gacgccgtta ccgggatcaa ttcatcacat tcggtgatgg taaaacccag tagatccgcc
    64381 acttcacggg cttccggggt aataatgctg gcgcgcagaa ccacggacat tgcctgttcg
    64441 ccgcgcgcgt gtgcttcacg aatatcattc gctgtgatga gttttttcac ctgcttcctc
    64501 ctgtttggtg agagatgcca gataatccac cagttgctgc acgctttgcg ggtcgtggct
    64561 gtttagctcg aaaatcggct cctcgaagcc cgtctcaagc aataactttc gcgttgcggc
    64621 gacgtcagca tccggcatgt ccgttttact gatgacggcg atttgccgtt tgctgacgcc
    64681 aatatccagc aacccggcag gcaaccgact ttccgggtca ttcgcgccgt ggacatagat
    64741 cagcatgtcc acatcctgta gcgtggtaat taaggcgtga taccagcggg gatggctaaa
    64801 atattcaccc ggcgtatcaa tatcgccctt atcattaaat tccacggcct gtgtttttct
    64861 ggcgagggta taattcccct gcaacgcatt aaataacgtt gttttccctg ccccgacggt
    64921 acccacaaac gcaattcgtt tcatgaatgc ctcgtattaa cttttggtca tttcacaaag
    64981 cgtataattt aataagcgac ctaaaccact gacggtttgt gataacgctt cttctactgc
    65041 gcccaccgag ccataaatca ccaatgcacc actgaagcgg tcgagaaatc cgatatgcac
    65101 atcggcggct ttcagcgcta aatcaccggc gatcatcgcc gtttcgccgg gagttagcgt
    65161 cataatgcca attgcgcccg catccggaac gccgattttc ttcgccagtt cctcgccggg
    65221 atgcgcgatg agatgcgcca gtgtgacctg tttgccgggc acaaattcct gaatgatgcg
    65281 ttctttatcc atgagtcgcc gcctgtgaaa aactttcgtg tatcgtgaca atttttcgtc
    65341 agagtaaata acaaaattcg gcaagcgcgt ttaaaaggtg aggtagatca ctaagaatgg
    65401 agagatattt actgttatga ataa
//
LOCUS       DAZKIP010000014        61639 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZKIP010000014 DAZKIP010000000
VERSION     DAZKIP010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 61639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.014"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(156..1631)
                     /gene="ampG"
                     /locus_tag="ACHOUK_001096"
     CDS             complement(156..1631)
                     /gene="ampG"
                     /locus_tag="ACHOUK_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFP9896585.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTAYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(1675..2253)
                     /gene="yajG"
                     /locus_tag="ACHOUK_001097"
     CDS             complement(1675..2253)
                     /gene="yajG"
                     /locus_tag="ACHOUK_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP9896586.1"
                     /translation="MFKKILFPLVALFMLAGCARPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            2558..2875
                     /gene="bolA"
                     /locus_tag="ACHOUK_001098"
     CDS             2558..2875
                     /gene="bolA"
                     /locus_tag="ACHOUK_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFP9896587.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            3219..4517
                     /gene="tig"
                     /locus_tag="ACHOUK_001099"
     CDS             3219..4517
                     /gene="tig"
                     /locus_tag="ACHOUK_001099"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFP9896588.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            4762..5385
                     /gene="clpP"
                     /locus_tag="ACHOUK_001100"
     CDS             4762..5385
                     /gene="clpP"
                     /locus_tag="ACHOUK_001100"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFP9896589.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            5511..6785
                     /gene="clpX"
                     /locus_tag="ACHOUK_001101"
     CDS             5511..6785
                     /gene="clpX"
                     /locus_tag="ACHOUK_001101"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFP9896590.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            6973..9327
                     /gene="lon"
                     /locus_tag="ACHOUK_001102"
     CDS             6973..9327
                     /gene="lon"
                     /locus_tag="ACHOUK_001102"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFP9896591.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILNTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            9518..9808
                     /gene="hupB"
                     /locus_tag="ACHOUK_001103"
     CDS             9518..9808
                     /gene="hupB"
                     /locus_tag="ACHOUK_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFP9896592.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            10000..11871
                     /gene="ppiD"
                     /locus_tag="ACHOUK_001104"
     CDS             10000..11871
                     /gene="ppiD"
                     /locus_tag="ACHOUK_001104"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP9896593.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADLQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDVAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKENLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            12022..12393
                     /gene="ybaV"
                     /locus_tag="ACHOUK_001105"
     CDS             12022..12393
                     /gene="ybaV"
                     /locus_tag="ACHOUK_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFP9896594.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QNKAAVPAKASDEEGSRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            12499..12897
                     /gene="fadM"
                     /locus_tag="ACHOUK_001106"
     CDS             12499..12897
                     /gene="fadM"
                     /locus_tag="ACHOUK_001106"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFP9896595.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFHWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(12949..13644)
                     /gene="queC"
                     /locus_tag="ACHOUK_001107"
     CDS             complement(12949..13644)
                     /gene="queC"
                     /locus_tag="ACHOUK_001107"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFP9896596.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKVLNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLK"
     gene            complement(13709..15409)
                     /gene="ybaE"
                     /locus_tag="ACHOUK_001108"
     CDS             complement(13709..15409)
                     /gene="ybaE"
                     /locus_tag="ACHOUK_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFP9896597.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEKALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSADGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMTDATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            15509..16327
                     /gene="cof"
                     /locus_tag="ACHOUK_001109"
     CDS             15509..16327
                     /gene="cof"
                     /locus_tag="ACHOUK_001109"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFP9896598.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGAISLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLHEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            16479..16937
                     /gene="decR"
                     /locus_tag="ACHOUK_001110"
     CDS             16479..16937
                     /gene="decR"
                     /locus_tag="ACHOUK_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFP9896599.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            16967..18739
                     /gene="mdlA"
                     /locus_tag="ACHOUK_001111"
     CDS             16967..18739
                     /gene="mdlA"
                     /locus_tag="ACHOUK_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFP9896600.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENREEAVDA"
     gene            18732..20513
                     /gene="mdlB"
                     /locus_tag="ACHOUK_001112"
     CDS             18732..20513
                     /gene="mdlB"
                     /locus_tag="ACHOUK_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFP9896601.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            20694..21032
                     /gene="glnK"
                     /locus_tag="ACHOUK_001113"
     CDS             20694..21032
                     /gene="glnK"
                     /locus_tag="ACHOUK_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFP9896602.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            21062..22348
                     /gene="amtB"
                     /locus_tag="ACHOUK_001114"
     CDS             21062..22348
                     /gene="amtB"
                     /locus_tag="ACHOUK_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFP9896603.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(22397..23257)
                     /gene="tesB"
                     /locus_tag="ACHOUK_001115"
     CDS             complement(22397..23257)
                     /gene="tesB"
                     /locus_tag="ACHOUK_001115"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFP9896604.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            23475..24047
                     /gene="ybaY"
                     /locus_tag="ACHOUK_001116"
     CDS             23475..24047
                     /gene="ybaY"
                     /locus_tag="ACHOUK_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFP9896605.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(24080..24391)
                     /locus_tag="ACHOUK_001117"
     CDS             complement(24080..24391)
                     /locus_tag="ACHOUK_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFP9896606.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            24554..24650
                     /gene="ffs"
                     /locus_tag="ACHOUK_001118"
     ncRNA           24554..24650
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHOUK_001118"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            24770..25123
                     /gene="ybaA"
                     /locus_tag="ACHOUK_001119"
     CDS             24770..25123
                     /gene="ybaA"
                     /locus_tag="ACHOUK_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFP9896607.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(25165..26715)
                     /gene="pdeB"
                     /locus_tag="ACHOUK_001120"
     CDS             complement(25165..26715)
                     /gene="pdeB"
                     /locus_tag="ACHOUK_001120"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFP9896608.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGADCSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASSKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(26879..27349)
                     /gene="ylaC"
                     /locus_tag="ACHOUK_001121"
     CDS             complement(26879..27349)
                     /gene="ylaC"
                     /locus_tag="ACHOUK_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFP9896609.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(27465..28016)
                     /gene="maa"
                     /locus_tag="ACHOUK_001122"
     CDS             complement(27465..28016)
                     /gene="maa"
                     /locus_tag="ACHOUK_001122"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFP9896610.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     ALGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(28188..28406)
                     /gene="hha"
                     /locus_tag="ACHOUK_001123"
     CDS             complement(28188..28406)
                     /gene="hha"
                     /locus_tag="ACHOUK_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFP9896611.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(28432..28806)
                     /gene="tomB"
                     /locus_tag="ACHOUK_001124"
     CDS             complement(28432..28806)
                     /gene="tomB"
                     /locus_tag="ACHOUK_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFP9896612.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(29352..32501)
                     /gene="acrB"
                     /locus_tag="ACHOUK_001125"
     CDS             complement(29352..32501)
                     /gene="acrB"
                     /locus_tag="ACHOUK_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFP9896613.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSIEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHNHTVDHH"
     gene            complement(32524..33717)
                     /gene="acrA"
                     /locus_tag="ACHOUK_001126"
     CDS             complement(32524..33717)
                     /gene="acrA"
                     /locus_tag="ACHOUK_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFP9896614.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            33859..34506
                     /gene="acrR"
                     /locus_tag="ACHOUK_001127"
     CDS             33859..34506
                     /gene="acrR"
                     /locus_tag="ACHOUK_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFP9896615.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            34634..37996
                     /gene="mscK"
                     /locus_tag="ACHOUK_001128"
     CDS             34634..37996
                     /gene="mscK"
                     /locus_tag="ACHOUK_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFP9896616.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(38035..38199)
                     /gene="rsmS"
                     /locus_tag="ACHOUK_001129"
     CDS             complement(38035..38199)
                     /gene="rsmS"
                     /locus_tag="ACHOUK_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFP9896617.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LQSDEASQSE"
     gene            complement(38213..38740)
                     /gene="priC"
                     /locus_tag="ACHOUK_001130"
     CDS             complement(38213..38740)
                     /gene="priC"
                     /locus_tag="ACHOUK_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFP9896618.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFNRHLFQTRAT
                     TLQACLDKAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREATAWSLREWDSAP
                     PQIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            38810..39187
                     /gene="ybaN"
                     /locus_tag="ACHOUK_001131"
     CDS             38810..39187
                     /gene="ybaN"
                     /locus_tag="ACHOUK_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFP9896619.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            39340..39891
                     /gene="apt"
                     /locus_tag="ACHOUK_001132"
     CDS             39340..39891
                     /gene="apt"
                     /locus_tag="ACHOUK_001132"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFP9896620.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGINKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            40020..41951
                     /gene="dnaX"
                     /locus_tag="ACHOUK_001133"
     CDS             40020..41951
                     /gene="dnaX"
                     /locus_tag="ACHOUK_001133"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFP9896621.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEDSIRPI"
     misc_feature    41284..41348
                     /gene="dnaX"
                     /locus_tag="ACHOUK_001133"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            42004..42333
                     /gene="ybaB"
                     /locus_tag="ACHOUK_001134"
     CDS             42004..42333
                     /gene="ybaB"
                     /locus_tag="ACHOUK_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFP9896622.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            42333..42938
                     /gene="recR"
                     /locus_tag="ACHOUK_001135"
     CDS             42333..42938
                     /gene="recR"
                     /locus_tag="ACHOUK_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFP9896623.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            43048..44922
                     /gene="htpG"
                     /locus_tag="ACHOUK_001136"
     CDS             43048..44922
                     /gene="htpG"
                     /locus_tag="ACHOUK_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFP9896624.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            45103..45747
                     /gene="adk"
                     /locus_tag="ACHOUK_001137"
     CDS             45103..45747
                     /gene="adk"
                     /locus_tag="ACHOUK_001137"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFP9896625.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            45879..46841
                     /gene="hemH"
                     /locus_tag="ACHOUK_001138"
     CDS             45879..46841
                     /gene="hemH"
                     /locus_tag="ACHOUK_001138"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFP9896626.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(46838..47797)
                     /gene="aes"
                     /locus_tag="ACHOUK_001139"
     CDS             complement(46838..47797)
                     /gene="aes"
                     /locus_tag="ACHOUK_001139"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFP9896627.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPPWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATKAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQVIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKVAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            47949..49253
                     /gene="gsk"
                     /locus_tag="ACHOUK_001140"
     CDS             47949..49253
                     /gene="gsk"
                     /locus_tag="ACHOUK_001140"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFP9896628.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(49386..51062)
                     /gene="ybaL"
                     /locus_tag="ACHOUK_001141"
     CDS             complement(49386..51062)
                     /gene="ybaL"
                     /locus_tag="ACHOUK_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFP9896629.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(51299..52519)
                     /gene="fsr"
                     /locus_tag="ACHOUK_001142"
     CDS             complement(51299..52519)
                     /gene="fsr"
                     /locus_tag="ACHOUK_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFP9896630.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            52737..54389
                     /gene="ushA"
                     /locus_tag="ACHOUK_001143"
     CDS             52737..54389
                     /gene="ushA"
                     /locus_tag="ACHOUK_001143"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFP9896631.1"
                     /translation="MKLLQRGVALALLTTFTLASEAALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKLGNPENFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGDHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(54426..54905)
                     /gene="ybaK"
                     /locus_tag="ACHOUK_001144"
     CDS             complement(54426..54905)
                     /gene="ybaK"
                     /locus_tag="ACHOUK_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFP9896632.1"
                     /translation="MTPAVKSLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(55109..55903)
                     /gene="ybaP"
                     /locus_tag="ACHOUK_001145"
     CDS             complement(55109..55903)
                     /gene="ybaP"
                     /locus_tag="ACHOUK_001145"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFP9896633.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDISLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQKLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            56041..56382
                     /gene="ybaQ"
                     /locus_tag="ACHOUK_001146"
     CDS             56041..56382
                     /gene="ybaQ"
                     /locus_tag="ACHOUK_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFP9896634.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(56597..59101)
                     /gene="copA"
                     /locus_tag="ACHOUK_001147"
     CDS             complement(56597..59101)
                     /gene="copA"
                     /locus_tag="ACHOUK_001147"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFP9896635.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADFDEAQALRLAAALEQGSSHPLAR
                     AILDKASDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            59363..60295
                     /gene="glsA"
                     /locus_tag="ACHOUK_001148"
     CDS             59363..60295
                     /gene="glsA"
                     /locus_tag="ACHOUK_001148"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFP9896636.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTSDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGELVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            60298..61590
                     /gene="ybaT"
                     /locus_tag="ACHOUK_001149"
     CDS             60298..61590
                     /gene="ybaT"
                     /locus_tag="ACHOUK_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP9896637.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
BASE COUNT        15216 a        15560 c        16385 g        14478 t
ORIGIN      
        1 ctgaatcgtg taagaaaaat agaatttata aatggcgcca ataatttcca aacgctaagc
       61 cgcacaaaag aacaaatatt aataagcgat gctgatgttt atttagccgt aataattacg
      121 gcgagtgatt actacagcta aataatattt acagattacg tcagatgcgt ttttcgtagc
      181 gccagataat ccagcaaacc accaagcacg acgccagaaa gggcgactaa aaccccgact
      241 tccagcagag caggcaggaa agagaagtgc gtcaaatcca gcgcgtccat cgtcaatagc
      301 agtaaccaca cggccaacag gctgacgccc gccgccagtg tccacatggc aaaggcataa
      361 cctgccggat atgcggtacg ggagataaag ttgtcattta ctcgtgtata ttcaagcgtc
      421 tggcggcaaa ccagcagcaa aataagccct ggtacggcag cggcgacgga gaatagatag
      481 aatgtcgacc agccgtgtgc ttcaacaaac caacccgcca cggggccgac ataaactcgc
      541 cctacagcag aaagcgctga gagcagggca aattgagtag cggaaaatga cttattacat
      601 agcgtcatta acagcgcgac aaaggctgat gtgcccatcc cgccacagag gttttcgaaa
      661 aagacggctg cgcccatgct gtagagatgc ttatcagtaa tcgacagcag ccagtaacca
      721 gcgttagacg caccttgtaa aatgccgaaa atcagcagtg cccggaacag tgacaggcgc
      781 tgcatcaaaa tcccaccgta caatgcgcca acaatggtcg ctagtaagcc aagcgttttg
      841 tttaccacgc ctacttcacc cgcatcaaac ccgacgccgc gaatcaaaaa cgtggttgtc
      901 aggctcatgg cgaatgcgtc gcccagctta tacagcacga taagaagcaa aataagccag
      961 gcattattgc gaccaaagaa atctcgcaga ggtgcaacaa ccgcttgttc cagcgttttg
     1021 ggcacaggaa tggtgtcggt tggttctggt gcaagcaacg tcgcgataat acaggggatc
     1081 aacagcgccg ccatcaacca gtacatgccc tgccagccca gccatttatc tgccagccac
     1141 agggctaggc cgccggaaac cagcatccct aaacggtaac ccagaacgct gattgccgcg
     1201 cccgcaccac gttcttctgc cgggagcaca tcggttttcc acgcatcgaa gacaatatcc
     1261 tgggaggcag agcaaaaagc gatcaccacc gccagcgccg ccatccagcg gagttgggtg
     1321 cctggttcga gaaaacccat cgccgcaatg gcgactaata acaggatttg cgtggcgagc
     1381 agccaaccgc gccgccgccc aaaaaatggg ggcgtgtagc ggtccatcag cggtgaccag
     1441 aggaatttaa aaacgtaagc ctggcctacc agagagaaga aaccaatggt tttgagatcg
     1501 atattctcga ccgtcatcca ggcctgtaag gtgccggatg tcagggcgag cggtagcccg
     1561 gaagcaaaac ccaggatcag caatatggct gaacgcggct gttgaaaaat acgtaaatat
     1621 tgactggaca tgggcgtcta caggcccggc ttgcaccggg ccagagggca gaaattaacg
     1681 cgcgttctgc ttgatgaatt cgtgaatgct ggtgtcctga gacatatcag cgatggtatc
     1741 ggtcagcaca ctgttaaccg catcggcgat atttttgttg gaggcctgga acgcaccttc
     1801 aacgttgtag ctggcacgat agtttttggt cattttgttg ccattctgcg cggtagcgat
     1861 gatggcgata tccgctttgg tagcgatgtt gtagcgcacg ttgccctggg acacgtcagc
     1921 atacagctgg ctaacgatga tttgcagatt aaccgggcca ttcggaccaa ccatgtaacc
     1981 acgcgcggtc atctgttttt ccagcacttc ttgcagcagg aaacgcagat cgcgggaggc
     2041 ggtcagggta acgatttgat tatcgcgggt gacttttgcc agcgcctgat cggtacgctg
     2101 atcggcacca ttaatgctta cggtgacgcc catcaggctt ggatcctgct gtggcagtgt
     2161 aatcgtcggg gaaacttcaa tagttgttgg cggtcttgcg catcctgcaa gcataaacag
     2221 agcaactaac gggaagagga tttttttgaa catgttcggg ctctcagaga ctcttaagcg
     2281 tgtttggtaa aaattcccgc catcataaca ttgccaacgg cgaggggaag taggtaaggc
     2341 atgtaaattc atcatgttga cgaaataatc gcccctggta aaagaaacgc tgatgcgagg
     2401 cctgtgtttc aatctttaaa tcagtaaact tcatacgctt gacggaaaaa ccaggacgaa
     2461 acctaaatat ttgttgttaa gctgcaatgg aaacggtaaa agcggctagt atttaaaggg
     2521 atggatgaca tctcagcgtt gtcggaggag atatttcatg atgatacgtg agcggataga
     2581 agaaaaatta agggcggcgt tccaacccgt attcctcgaa gtagtggatg aaagctatcg
     2641 tcacaatgtc ccagccggct ctgaaagcca ttttaaagtt gtgctggtca gcgatcgttt
     2701 tacgggtgaa cgttttctga atcgtcatcg aatgatttac agtactttag cggaggaact
     2761 ctctactacc gttcatgcgc tggctctgca tacttacact attaaggagt gggaagggtt
     2821 gcaggacacc gtctttgcct ctcctccctg tcgtggagca ggaagcatcg cgtaaaaacg
     2881 catttgcaac tgtcggcgct tttccagtat gttgctaaag attttatgaa aaacggcctg
     2941 cgggccgttt tgttttgtct ggattttgcg ctttttgccc agcattcaga cgaaaattgc
     3001 ccgggaattg tgaaaaaata cgcgacagcg cgcaataacc gttctcgact cataaaagtg
     3061 atgccgctat aatgccgcgt cctatttgaa tgctttcggg atgattctgg taacagggaa
     3121 tgtgattgat tataagaaca tcccggttcc gcgaagccaa caacctgtgc ttgcggggta
     3181 agagttgacc gagcactgtg attttttgag gtaacaagat gcaagtttca gttgaaacca
     3241 ctcaaggcct tggccgccgt gtaacgatta ctatcgctgc tgacagcatc gagaccgctg
     3301 ttaaaagcga gctggtcaac gttgcgaaaa aagtacgtat tgacggcttc cgcaaaggca
     3361 aagtgccaat gaatatcgtt gctcagcgtt atggcgcgtc tgtacgccag gacgttctgg
     3421 gtgacctgat gagccgtaac ttcattgacg ccatcattaa agaaaaaatc aatccggctg
     3481 gcgcaccgac ttatgttccg ggcgaataca agctgggtga agacttcacc tactctgtag
     3541 agtttgaagt ttatccggaa gttgaactgc agggtctgga agcgatcgaa gttgaaaaac
     3601 cgatcgttga agtgaccgac gctgacgttg acggcatgct ggatactctg cgtaaacagc
     3661 aggcgacctg gaaagaaaaa gacggcgctg ttgaagcaga agaccgcgta accatcgact
     3721 tcaccggttc tgtagacggc gaagagttcg aaggcggtaa agcgtctgat ttcgtactgg
     3781 cgatgggcca gggtcgtatg atcccgggct ttgaagacgg tatcaaaggc cacaaagctg
     3841 gcgaagagtt caccatcgac gtgaccttcc cggaagaata ccacgcagaa aacctgaaag
     3901 gtaaagcagc gaaattcgct atcaacctga agaaagttga agagcgtgaa ctgccggaac
     3961 tgactgcaga attcatcaaa cgtttcggcg ttgaagatgg ttccgtagaa ggtctgcgcg
     4021 ctgaagtgcg taaaaacatg gagcgcgagc tgaagagcgc catccgtaac cgcgttaagt
     4081 ctcaggcgat cgaaggtctg gtaaaagcta acgacatcga tgtaccggct gcgcttattg
     4141 acagcgaaat cgacgttctg cgtcgccagg ctgcacagcg tttcggtggc aacgaaaaac
     4201 aagctctgga actgccgcgc gaactgttcg aagaacaggc taaacgccgc gtagttgttg
     4261 gcctgctgct gggcgaagtt atccgcacca acgagctgaa agctgacgaa gagcgcgtga
     4321 aaggcctgat cgaagagatg gcttcagcgt acgaagatcc gaaagaagtt atcgagttct
     4381 acagcaaaaa caaagaactg atggacaaca tgcgcaatgt tgctctggaa gaacaggctg
     4441 ttgaagctgt actggcgaaa gcgaaagtga ctgaaaaaga aaccactttc aacgagctga
     4501 tgaaccagca ggcgtaattt acgcagcata acgcgctaaa ttcgcacaaa ggcccgtcac
     4561 cgccaggtgg tgggcttttt ttgtcatgaa ttttgcatgg aaccgtgcga aaagcctctt
     4621 tcggtgttag cgtaacaaca aaagattgtt atgcttgaaa tatggtgatg ccgtacccat
     4681 aacacaggga ctagctgata atccgtccat aaggttacaa tcggtacagc aggttttttc
     4741 aattttatcc aggagacgga aatgtcatac agcggcgaac gagataactt tgcaccccat
     4801 atggcgctgg tgccgatggt cattgaacag acctcacgtg gtgagcgctc ttttgatatc
     4861 tattctcgtc tacttaagga acgcgtcatt tttctgactg gccaggttga agaccatatg
     4921 gctaacctga ttgtggctca gatgctgttc ctggaagcag aaaacccaga aaaagatatc
     4981 tatctgtaca ttaactcccc aggcggggtg atcactgccg ggatgtctat ctatgacacc
     5041 atgcagttta tcaagcctga tgtcagcacc atctgtatgg gccaggcggc ctcgatgggc
     5101 gctttcttgc tgaccgcagg ggcaaaaggt aaacgttttt gcctgccgaa ttcgcgcgtg
     5161 atgattcacc aaccgttggg cggctaccag ggccaggcga ccgatatcga aattcatgcc
     5221 cgtgaaattc tgaaagttaa agggcgtatg aatgaactta tggcgcttca tacgggtcaa
     5281 tcattagaac agattgaacg tgataccgag cgcgatcgct tcctttccgc ccctgaagcg
     5341 gtggaatacg gtctggtcga ttcgattctg acccatcgta attgatgcca gaggcgcaac
     5401 tgtgccgcta tacttatcca gggcggcaca acgctgtaag cggcttgcgc ctgagaatgg
     5461 catttgcgtc gtcgtgtgcg gcacaaagaa caaagaagag gttttgaccc atgacagata
     5521 aacgcaaaga tggctcaggc aaattgctgt attgctcttt ttgcggcaaa agccagcatg
     5581 aagtgcgcaa gctgattgcc ggtccatccg tgtatatctg cgacgaatgt gttgatttat
     5641 gtaacgacat cattcgcgaa gagattaaag aagttgcacc gcatcgtgaa cgcagtgcgc
     5701 taccgacgcc gcatgaaatt cgtaaccacc tggacgatta cgttatcggc caggaacagg
     5761 cgaaaaaagt gctggcggtc gcggtataca accactacaa acgtctgcgc aacggcgata
     5821 ccagcaatgg cgtcgagttg ggcaaaagta acattctgct gatcggtccg accggttccg
     5881 gtaaaacgct gctggctgaa acgctggcgc gcctgctgga cgtcccgttc accatggccg
     5941 acgcaaccac gctgaccgaa gccggttatg tgggcgaaga cgttgaaaac atcattcaga
     6001 agctgttgca aaagtgcgat tacgacgtac agaaagcgca gcgcgggatt gtctacatcg
     6061 atgaaatcga caagatttct cgtaagtcag acaacccgtc tattacccgt gacgtttccg
     6121 gtgaaggcgt acagcaggca ctgttgaaac tgatcgaagg tacggtagct gctgttccac
     6181 cgcaaggtgg acgtaaacat ccgcagcagg aattcttgca ggttgatacc tctaagatcc
     6241 tgtttatttg tggcggtgcg tttgccggtc tggataaagt gatttcccat cgtgtagaaa
     6301 ccggctccgg cattggtttt ggcgcgacgg taaaagcgaa gtccgacaaa gcaagcgaag
     6361 gcgagctgct ggcgcaggtt gaaccggaag atctgatcaa gtttggtctt atccctgagt
     6421 ttattggtcg tctgccggtt gtcgcaacgt tgaatgaact gagcgaagaa gctctgattc
     6481 agatcctcaa agagccgaaa aacgccctga ccaagcagta tcaggcgctg tttaatctgg
     6541 aaggcgtgga tctggaattc cgtgacgagg cgctggatgc tatcgctaag aaagcgatgg
     6601 cgcgtaaaac cggtgcccgt ggcctgcgtt ccatcgtaga agccgcactg ctcgatacca
     6661 tgtacgatct gccgtccatg gaagatgtcg aaaaagtggt tatcgacgag tcggtaattg
     6721 atggtcaaag caaaccgttg ctgatttatg gcaagccgga agcgcaacag gcatctggtg
     6781 aataattaac cattcccata caattagtta accaaaaagg ggggatttta tctccccttt
     6841 aatttttcct ctattctcgg cgttgaatgt gggggaaaca tccccatata ctgacgtaca
     6901 tgttaataga tggcgtgaag cacagtcgtg tcatctgatt acctggcgga aattaaacta
     6961 agagagagct ctatgaatcc tgagcgttct gaacgcattg aaatccccgt attgccgctg
     7021 cgcgatgtgg tggtttatcc gcacatggtc atccccttat ttgtcgggcg ggaaaaatct
     7081 atccgttgtc tggaagcggc gatggaccat gataaaaaaa ttatgctggt cgcgcagaaa
     7141 gaagcttcaa cggatgagcc gggtgtaaac gatcttttca ccgtcgggac cgtggcctct
     7201 atattgcaga tgctgaaact gcctgacggc accgtcaaag tgctggtcga ggggttacag
     7261 cgcgcgcgta tttctgcgct ctctgacaat ggcgaacact tttctgcgaa ggcggagtat
     7321 ctggagtcgc cgaccattga tgagcgagaa caggaagtgc tggtgcgtac tgcaatcagc
     7381 cagttcgaag gctacatcaa gctgaacaaa aaaatcccac cagaagtgct gacgtcgttg
     7441 aatagcatcg acgatccggc gcgtctggcg gataccattg ctgcacatat gccgctgaaa
     7501 ctggctgaca aacagtccgt tctggagatg tccgacgtta acgaacgtct ggaatatctg
     7561 atggcaatga tggaatcgga aatcgatctg ctgcaggttg agaaacgcat tcgcaaccgc
     7621 gttaaaaagc agatggagaa atcccagcgt gagtactatc tgaacgagca aatgaaagct
     7681 attcagaaag aactcggtga gatggacgac gcgccggacg aaaacgaagc cctgaagcgc
     7741 aaaatcgacg cggcgaagat gccgaaagag gcaaaagaga aagcggaagc agagttgcag
     7801 aagctgaaaa tgatgtctcc gatgtcggca gaagcgaccg tagtgcgtgg ttatatcgac
     7861 tggatggtac aggtaccgtg gaatgcgcgc agcaaggtca aaaaagacct gcgtcaggcg
     7921 caggaaatcc ttaataccga ccattatggt ctggagcgcg tgaaagatcg catccttgag
     7981 tatcttgcgg ttcaaagccg tgtcaacaaa atcaagggac cgatcctttg cctggtaggg
     8041 ccgccggggg taggtaaaac ctccctgggt cagtccattg ccaaagccac cgggcgtaaa
     8101 tatgtccgta tggcgctggg cggcgtgcgt gatgaagcgg aaatccgtgg tcaccgccgt
     8161 acttacatcg gttctatgcc gggtaaattg atccagaaaa tggcgaaagt gggcgttaaa
     8221 aacccgctgt tcctgctcga tgagatcgac aaaatgtctt ctgacatgcg tggcgatccg
     8281 gcttccgcac tgcttgaagt gctggatcca gagcagaacg tggccttcag cgatcactac
     8341 ctggaagtgg attacgatct cagcgacgtg atgtttgtcg cgacgtcgaa ctccatgaac
     8401 attccggcac cactgctgga tcgtatggaa gtgattcgcc tctccggtta taccgaagat
     8461 gaaaaactga acatcgccaa acgtcacctg ctgccgaagc agattgaacg taatgcactg
     8521 aaaaaaggtg agctgaccgt cgacgatagc gccattatcg gcattattcg ttactacacc
     8581 cgtgaggcgg gcgtgcgtgg tctggagcgt gaaatctcca agctgtgccg taaagcggtt
     8641 aagcagttac tgctcgataa gtcattaaaa catatcgaaa ttaacggcga caacctgcat
     8701 gactaccttg gtgttcagcg tttcgactat ggtcgcgctg ataacgaaaa ccgtgtcggt
     8761 caggtaaccg gtctggcgtg gacggaagtg ggcggtgact tgctgaccat tgaaaccgca
     8821 tgtgttccgg gtaaaggcaa actgacctat accggttcgc tcggcgaagt gatgcaggag
     8881 tctattcagg ctgcgttaac ggtggttcgc gcgcgtgcgg aaaaactggg gatcaaccct
     8941 gatttttacg aaaaacgtga catccacgtc cacgtaccgg aaggtgcgac gccgaaagat
     9001 ggtccgagtg ccggtattgc tatgtgcacc gcgctggttt cttgcctgac cggtaacccg
     9061 gttcgtgccg atgtggcaat gaccggtgag atcactctgc gtggtcaggt actgccgatc
     9121 ggtggtttga aagaaaaact actggcagcg catcgcggcg ggattaaaac agtgttaatt
     9181 ccgttcgaaa ataaacgcga tctggaagag attcctgaca acgtaattgc cgatctggac
     9241 attcatcctg tgaagcgcat tgaggaagtt ctgactctgg cgctgcaaaa tgaaccgtct
     9301 ggcatgcagg ttgtgactgc aaaatagtga cctcgcgcaa aatgcactaa taaaaacagg
     9361 gctggcaggc taattcgggc ttgccagcct ttttttgtct cgctaagtta gatggcggat
     9421 cgggcttgcc cttattaagg ggtgttgtaa ggggatggct ggcctgatat aactgctgcg
     9481 cgttcgtacc ttgaaggatt caagtgcgat ataaattata aagaggaaga gaagagtgaa
     9541 taaatctcaa ttgatcgaca agattgctgc aggggctgat atctctaaag ctgcggctgg
     9601 ccgtgcgtta gatgctatta ttgcttccgt aactgaatct ctgaaagaag gggatgatgt
     9661 agcactggta ggttttggta cttttgccgt taaagagcgt gctgcccgta ctggccgcaa
     9721 cccgcagacc ggtaaagaga tcaccatcgc tgccgctaaa gtaccgagct tccgtgcagg
     9781 taaagcactg aaagacgcgg taaactaagc gttgtcccca gtggggatgt gacgaagttc
     9841 aagggcgcat ctactgatgt gcctttttta tttgtattcg gtgactttct gcgtcttgtg
     9901 ggctgacaat tgcccccgtt tcttgtcaca ataggccttt gcgcgcatcg atacgttgcg
     9961 tgaggtacac agtcatctac agcggagtgt tgttacacca tgatggacag cttacgcacg
    10021 gctgcaaaca gtctcgtgct caagattatt ttcggtatca ttatcgtgtc gttcatattg
    10081 accggcgtga gtggttacct gattggcgga ggcaataact acgccgcaaa agtgaatgac
    10141 caggaaatca gccgtgggca attcgagaat gccttcaaca gcgagcgtaa tcgcatgcag
    10201 caacagctgg gcgatcaata ctccgagctg gcagcgaacg aaggctatat gaaaaccctg
    10261 cgtcaacagg tgctgaatcg tctgatcgac gaggcgctgc tggatcagta cgcacgtgag
    10321 ctgaaactgg gtatcagcga tgagcaggtt aaacaggcga ttttcgcgac cccagccttc
    10381 caggttgatg gcaaatttga taacagccgc tataacggta tcctcaacca gatggggatg
    10441 accgccgatc agtacgccca ggcgctgcgt aaccagctca ctacccaaca gctgattaac
    10501 ggcgttgctg gtaccgattt tatgctgaaa ggtgaaaccg acgagctggc ggcactggtc
    10561 gctcaacaac gcgtggtgcg tgaagcgact atcgatgtta acgcgctggc ggcgaagcag
    10621 cctgtgaccg aacaggaaat tgccagctac tacgaacaaa acaaaaacaa tttcatgacg
    10681 ccggaacaat tccgcgtgag ttacatcaag ctggatgccg caacgatgca gcaaccggtt
    10741 agcgatgcgg atctccagag ctactacgat cagcatcagg atcaattcac ccagccgcag
    10801 cgtacccgtt acagcatcat ccagaccaaa actgaagatg aagcgaaagc ggtacttgat
    10861 gagctgaata aaggcggtga ttttgctgca ttagccaaag aaaaatctgc cgatattatc
    10921 tctgctcgta acggcggcga tatgggttgg ttagaagatg ccactatccc ggatgaactg
    10981 aaaaatgctg gtctgaaaga aaaaggccaa ctgtctggtg tcatcaaatc ttcggtcggt
    11041 ttcctgattg tacgtctgga cgacattcag ccagcgaaag tgaaatcgtt agacgaagta
    11101 cgtgacgatg tcgcggcgaa agtgaaacac gaaaaagccc tcgatgcgta ctacgcgctg
    11161 cagcagaaag tgagcgatgc ggcaagcaac gacaccgagt ctctggccgg tgcagagcaa
    11221 gctgccggcg ttaaagccac tcagacgggt tggttcagca aagagaacct gccggaagag
    11281 ttgaacttca agccggttgc tgacgctatc tttaacggcg gtctggtagg tgaaaacggc
    11341 gcgccgggca tcaactctga catcatcacc gtagacggcg accgcgcatt cgtgctgcgc
    11401 atcagcgagc acaaaccgga agcggtgaaa ccgttggcag atgttcagga acaagttaag
    11461 gcactggttc agcacaacaa agctgaacaa caggcgaaag tggatgctga gaaactgctg
    11521 gttgatttga aagccggcaa aggtgcggaa gctatgcagg ctgccggtct gaaatttggc
    11581 gagccgaaaa ccttaagccg ttccggtcgt gacccgatta gccaggcggc gtttgcactg
    11641 ccactgccag cgaaagacaa accgagctac ggtatggcga ccgatatgca aggtaatgtg
    11701 gttctgctgg cgctggatga agtgaaacaa ggttcaatgc cggaagatca gaaaaaagcg
    11761 atggtgcagg gtatcaccca gaacaacgca caaatcgtct ttgaagctct gatgagtaac
    11821 ctgcgtaaag aggcgaaaat caaaattggc gatgcgttgg aacagcaata atcctgaagc
    11881 cgcctcgcaa aaaaatgctt tgcagctgta acaagaaaag gtcgctttcg cggccttttc
    11941 catttctgaa cattgccatt tgtttactgt tttcactgcc gttaaggtga ttccactgtt
    12001 aacaaacaag gagaaaacag tatgaaacac ggaattaaag cactgctcat taccctgtcc
    12061 ctggcctgtg ccggaatgtc tcatagcgcg ctggcggcag cttctgtggc gaaaccgacg
    12121 gcggtagaaa ccaaagcgga agctcctgca gcacaaaata aagcagcagt accggcgaaa
    12181 gccagtgacg aagaaggctc ccgggtcagc attaataatg ccagcgcgga agagctagcc
    12241 cgcgcgatga atggcgttgg cctgaagaaa gcgcaagcga ttgtcagtta tcgcgaagag
    12301 tacggtccgt ttaaaacggt ggaggatcta aagcaggtgc cggggatggg caattcgctg
    12361 gtggaacgta atctggcggt attaaccctg taattaattt gcatagtggc aattttttgc
    12421 cagactgaag aggtcatacc agttatgacc tctgtactta taacagtttt tataaaaaca
    12481 acgtaaggtt attgcgctat gcaaacacaa atcaaagttc gtggatatca tctcgacgtt
    12541 taccagcacg taaacaacgc ccgctacctt gaatttctcg aagaagcccg ctgggatggg
    12601 ttggaaaata gcgacagttt tcactggatg acggcccata acatcgcttt cgtggtggtg
    12661 aatatcaata ttaactatcg tcgtccggcg gtattaagcg acttgttaac cattacgagc
    12721 cagttacagc aattaaacgg taaaagcggc atcttaagcc aggtcattac actggagccg
    12781 gaagggcagg tggtagcgga tgcgcttatt acgtttgttt gtattgatct taaaacgcag
    12841 aaagcattag ctctggaagg ggaattgcgc gaaaagctgg agcagatggt taagtaaacg
    12901 ttttgtggtg ccggatgctc aagccgcatc cggcgacacc cggaataatt acttcaaccc
    12961 ggttttctgc ttcatcgctg ccatcaccgt cggtttatcg gccagataat gattcaaacc
    13021 gttggcgcgt aaattacatg ccgcacaatg accgcaaccg tcgcctttaa tgccgttata
    13081 gcaggtcaac gtttcgttac ggactaaatc cagtttgccg taataatctg ccagcgccca
    13141 ggtttccgct ttatcaatcc acatcagcgg cgtttcaaaa cgaatatctt tcgccatgcc
    13201 caaactgacg gcatggttta gtactttcac aaactcatcg cggcaatccg ggtagccgga
    13261 gaaatccgtt tcgcagacgc cggtaattac ggcttctgct tttacctgat acgcatatat
    13321 tgccgccagc gtcaggaaca aaatattacg ccctgggaca aacgtattcg ggatgccatc
    13381 ggcttcaggt tcataatcag gcaccggaat gctgtcacgc gtcaggctgc tgaccgccag
    13441 ctcgttgagc agcgttacat caagaacctt atgcgcgcgt gcccccagtt tcagcgccag
    13501 ttcgcgtgcc acgtcgattt ctgcgcgatg gcgctgaccg taatcgaacg tcacgcaatg
    13561 gacttcatca tattgttgta atgcctgcac cagacaggtg gtggaatcct ggcctccact
    13621 gaacacaacg acagcacgtt tcatagataa tcctgcttga acaataaaag cgttatggta
    13681 acgcctgcga ttaacccgga ccagctattc attgcgacgg cgcgggaagc caggcttcgg
    13741 taaattcaaa ccagccgcgc ggtgtcagtc gcacaccgtt cacgccggga ggggcactaa
    13801 tgcgatagtg atagttgaac agcggcgtca gcgtcgcatc tgtcattaac tggctaaaaa
    13861 ccgctttcag ggcattaaat cggttttcct catcaggcat tacttgcacc gcgtccagtg
    13921 tcgattgtag atgtgcgtat gctggagcgt cgaaaacatg tggccacagc ggatcgcagc
    13981 gcagccattg ctccagagta tattccggtg cttcgccaat taatctgtcg cccatcatga
    14041 ggtctgcgtg tgccagtagg gtcgtgtcgt cccagttttt tgcgttatga aaaataattg
    14101 tgagttcaca gccttccgct gccagtgtcg cctgtaggcg ttctgccatg gtatgaagtt
    14161 ctatcgggag gtgataaacc agcgtcaagg ttttcggtag tttgacttca tccggtactt
    14221 gccaatgcgg aatagtccag cctggcagta atgcatgact ggcggtgatc aggttttctc
    14281 cgacttctaa cgtttgtaat aaaccggatt gatgaataat ggagatcact tttcgcgcct
    14341 gccagaggga gagtcgggga cttttgcgca acgttaaata gcaaaaacct aaactgatgc
    14401 cactactgac ctggctgacc cgttgcagct cctccggttt gccgatggtg atttgcacgg
    14461 gatgccgaca actggttccc aaatcttttt cgaaaagcgg cggagttatc cagtactcaa
    14521 ccgctttaag cagcggatga cgtaaatggt aataatcatg gctttccagg cgcaccagtt
    14581 cagctgtgaa ttgtgttaag cgaaaaggac ccgtgccaat cagtgggaat tgcggatgcg
    14641 ccagatggct gcaatagctc gccagccggt gcgcaagcca gtaatcgggg cgatgtaaaa
    14701 agaaggtcag acactgcgga tgggtgacct caatacgctt cacgctaata aataattgat
    14761 ccagtgctgg cagttgtaac agcatcaata atcgctggtg taagtgtgag gcttttactg
    14821 catcgccgtt atgccagtgt agggttgaac gaagataaaa gtcccagcgt aacccgtcag
    14881 cagaggtttc ccagtgatgc gctaaatcgc caatcgggcg ctgggtatta ttatcgaagc
    14941 gggtcaggcc ggaaaatatc tgtccggcga gatgctgctc ggcacggccg ggcaaaaagc
    15001 ccggttgtag cggttcgagc gggcgatagt agggaatacg caacgtgggt gtatcgtttt
    15061 gccattgtcc gcccataaac ggctgtaaca gagtgcgcag ctcacctggg gccagttgcg
    15121 ccagctccag cacatcttgc tgctttccgg tttccagtgc cttttccatc atcgcattgc
    15181 gtagcgattc tggcgtgacc agaaagcgta attgtccgcg ctttccgcgt cctgactgcg
    15241 cctgccactc cagccatccc gcttcctgtg cctgacgcaa cagcgtacga acatggcgtt
    15301 cgctgcaaaa acagcgttcg gccagttcgc tgacggtgac ggtttgcggc tttccggcgg
    15361 aaggttgcca cagacgttga tactggttaa ggcggttgag cagtcgcata aaaacccgga
    15421 acaatattat ttaactattc actattactt ccgtatatat caggtgatac tcaatcacca
    15481 ttaaccgtgt cacagagtgg agaaagaaat ggctcgtctg gcagcatttg atatggatgg
    15541 cactttattg atgcctgacc atcatttagg tgagaaaacc ctctctactt tggcgcgact
    15601 gcgtgaacgc gacattaccc tcacttttgc cacggggcgt catgcgctgg agatgcagca
    15661 tattctcggg gcgatatcgc tggatgcgta tttgattacc ggcaacggaa cgcgcgtgca
    15721 ttctctggaa ggtgaacttt tacatcgtga tgatttacct gcggatgtcg cggagctggt
    15781 gctgtatcag caatgggata cccgagccag catgcatatt ttcaatgacg acggttggtt
    15841 taccgggaaa gagatccctg cgttgttgca ggcatttgtc tatagcggtt ttcgttatca
    15901 aataatcgat gtcaaaaaaa tgccactcgg cagcgtcacc aagatctgct tctgtggcga
    15961 tcacgacgat cttacacgtt tgcagatcca gctacacgaa gcattaggcg agcgtgcaca
    16021 tttgtgtttt tccgccacgg attgccttga agtgctgccg gtgggctgca ataaaggcgc
    16081 tgcactgacg gtgctgaccc aacatttagg tttatcgttg cgcgattgca tggcctttgg
    16141 tgatgcgatg aacgatcgcg aaatgttagg cagcgtcggt agcggattta ttatgggcaa
    16201 tgcgatgccg caactgcgcg cggagctccc gcatttaccg gtgattggac attgccgaaa
    16261 tcaggctgtc tctcactatt tgacgcactg gctggactat ccacatctac cttattcccc
    16321 cgaataacga gatcccttcc agcaccgggc aattgcccgg tttttttgcg ttgaatttgt
    16381 cattttgtgc cgtggtgttt aaaccgcaca gaataaattg tcgcgatttc acctttaaaa
    16441 tagaattaaa agagaaaaaa attctccgtg gaagggctat gttagataaa attgaccgta
    16501 agctgctggc cttactacag caggattgca ccctctcttt gcaggcactg gctgaagccg
    16561 ttaatctgac aaccaccccc tgctggaagc gcctgaaacg gctggaggac gacggtatcc
    16621 ttatcggcaa agtcgccctg ctggatccgg aaaaaatagg cctcggcctg accgcctttg
    16681 tgctgataaa aacgcaacat cacagcagcg aatggtattg ccgctttgtc acggtggtta
    16741 ccgaaatgcc agaagtgctg gggttctggc gcatggctgg tgaatacgat tatctgatgc
    16801 gcgtccaggt tgccgacatg aaacgctacg acgagtttta taagcgtctg gtaaacagcg
    16861 tgccggggct gtcggacgtc acttccagct tcgcgatgga acagattaaa tacaccactt
    16921 ctttacccat cgaataaata tccagaatca ggtcaggaca caacgcgtgc gattatttgc
    16981 tcaattaagc tggtatttcc gtcgggaatg gcgtcgctat ctcggggctg tcgccttgct
    17041 tgtcattatc gcgatgctgc aactggttcc accaaaagtg gttggtattg ttgtcgatgg
    17101 cgtgacagaa caacacttta ctaccgggca gatcctgatg tggatcgcca ccatggtgct
    17161 gattgccgtt gtggtttatc tcctgcgtta cgtctggcgc gtattgctgt ttggtgcgtc
    17221 ttatcaactg gctgttgaac tgcgtgaaga ttattaccgt cagctaagcc ggcagcatcc
    17281 tgagttttac ctgcgtcatc gcacgggtga cctcatggct cgtgcgacta atgacgtcga
    17341 tcgcgtcgtg tttgccgccg gagaaggggt gctgacgctg gtggattcac tggtaatggg
    17401 ctgcgctgtg ttgattatga tgtctacgca aattagctgg cagttgacct tatttgccct
    17461 gttaccgatg ccagtgatgg cgatcatgat taagcgcaac ggcgatgctt tgcatgaacg
    17521 ctttaagctg gcacaggcgg cgttttccag tcttaatgac cgcacccagg aaagcctcac
    17581 cagtatccgc atgatcaaag cctttggtct ggaagatcgc cagtcggcgt tatttgccgc
    17641 ggatgccgaa gataccggca aaaaaaacat gcgggtggcg cgtattgatg ctcgtttcga
    17701 cccgaccatc tatatcgcga ttggtatggc gaacttgctg gcgattggcg gcggtagttg
    17761 gatggtggtg cagggcagtt taacgctggg ccagctcacc agttttatga tgtatttagg
    17821 tctgatgatt tggccaatgc tggcgctggc atggatgttt aacattgtgg aacgtggtag
    17881 tgctgcgtac agccgtattc gcgcgatgct ggcggaagcg ccggtggtga acgatggtag
    17941 cgaacccgtg ccggaagggc gtggcgaact ggatgtaaat attcaccagt tcacgtatcc
    18001 gcagactgac catcctgcgc tggaaaacgt caatttcgcc ctgaaacccg gtcagatgct
    18061 gggtatctgc gggccgactg gttccggcaa aagtaccctg ttgtcgctca ttcagcgtca
    18121 tttcgacgtc agcgaggggg atattcgctt tcatgatatt cctctgacga agttacaact
    18181 cgatagctgg cgtagccgcc tggcggtagt tagccagacg ccattccttt tttctgacac
    18241 cgtggcgaat aacatcgcgc tgggttgccc gaatgccacc cagcaagaga ttgagcatgt
    18301 cgcgcggtta gccagcgtac atgacgatat tttgcgtcta ccgcaaggtt acgatacaga
    18361 ggtgggcgag cgcggtgtga tgctttccgg cgggcaaaaa cagcgtatct ccattgctcg
    18421 tgcgttatta gtcaacgcgg aaatcctcat ccttgatgat gcgctttcgg cggtggacgg
    18481 acgcactgag caccagatcc tgcataacct gcgtcagtgg gggcagggaa gaacggtaat
    18541 catcagtgcc catcgccttt ctgcactgac ggaagccagt gaaattattg tgatgcagca
    18601 cggacatatc gcccagcgtg gcaatcatga tgtgctggca caacaaagcg gctggtatcg
    18661 cgatatgtat cgctatcaac aactggaggc ggcgctcgac gacgctccgg aaaatcgcga
    18721 ggaggccgtc gatgcgtagt tttagccaac tgtggccgac tctcaagcgc ctgttagcgt
    18781 acggttcacc gtggcgtaaa ccgctgggga ttgcggtcct gatgatgtgg gttgcggcgg
    18841 cggcagaagt cagtgggccg ctgcttatca gctattttat cgacaatatg gtagcgaaaa
    18901 ataacctgcc gttgaaagtg gttgcagggc tggctgcggc gtatgttggg ctgcaactgt
    18961 ttgccgccgg gctacattac gcgcagtcgc tgctgtttaa tcgggcggca gtaggcgtag
    19021 tgcaacagtt gcgtaccgac gtgatggatg ctgcgttacg ccagccatta agcgagtttg
    19081 atacccaacc cgtcgggcag gtgatttccc gcgtcactaa tgacactgaa gtgatccgcg
    19141 atctctacgt taccgtagtg gcaactgtcc tgcgcagtgc cgcactggtg ggcgcgatgc
    19201 tggtggcgat gttcagcctc gactggcgaa tggcgctggt ggcgataatg attttcccgg
    19261 tggtgctggt ggtaatggtg atataccagc gttacagcac gccaattgtc cgtcgtgtgc
    19321 gcgcctatct ggcggatatc aacgacggct ttaacgaaat catcaatggc atgagcgtta
    19381 tccagcagtt tcgtcagcag gcgcgatttg gcgaacgtat gggtgaggcc agtcgttcac
    19441 actatatggc gaggatgcaa accctgcgcc tcgacggttt tctgctgcgt ccgctgctga
    19501 gtctgttttc atcgctcatt ctctgtggct tgttgatgct gtttggcttc tccgccagcg
    19561 gcaccattga agtgggcgtg ttgtatgcgt ttatcagcta tcttgggcga cttaacgaac
    19621 ccttaatcga actgaccacg cagcaggcga tgctgcaaca ggctgttgtt gctggtgagc
    19681 gcgtgtttga gctgatggac ggaccgcgcc agcaatatgg caatgatgat cgcccgttac
    19741 agagtggcac catcgaagtc gataacgtgt catttgctta tcgcgatgac aatctggtgc
    19801 taaagaacat taatctctct gtgccttcgc gtaactttgt ggcgctggtc gggcataccg
    19861 gcagtggtaa aagtaccctc gccagtttat tgatgggcta ttacccgcta acggaaggtg
    19921 agattcgcct tgatggtcgt ccattaagtt cgctaagtca cagcgcgctg cgccagggcg
    19981 tggcaatggt gcagcaagat ccggtggtgc tggcggatac cttcctcgcc aacgtgacgc
    20041 tggggcggga tatctccgaa gaacgcgtct ggcaggcgct ggaaaccgtg caactggcgg
    20101 agctggcacg cagtatgagc gacgggattt atacgccgct gggcgagcag gggaataatc
    20161 tctctgtcgg gcaaaagcaa ctgctggcac tggcgcgcgt gctggtcgag acgccgcaaa
    20221 tcctgatcct tgatgaggca accgccagca ttgactccgg tactgaacag gcgattcaac
    20281 atgctctggc ggcggtgcgt gaacatacca cgctggtagt gattgctcac cgcttatcga
    20341 ctattgttga tgccgacact attctggtgc ttcatcgtgg gcaagccgtg gagcagggca
    20401 ctcaccagca actgctggcg gcccagggac gctactggca gatgtatcaa ctgcaacttg
    20461 cgggcgaaga gcttgcagcc agcgtgcgtg aagaagaatc attgagcgcc tgaatagcgc
    20521 aatatctcat cgttggtgca aaaatgtaac gcactgtgca ctgtcatagt gcgttttcat
    20581 tttcaatctt cttaacttcc tgctctcttt ctcgtttttc atttctggca caccgcttgc
    20641 aataccttct tcgtgtagca gaaccattac cgaattctga ccggagggga tctatgaagc
    20701 tggtgaccgt gataatcaaa ccattcaagc tggaagacgt tcgtgaagcg ttatcttcca
    20761 ttggtattca gggcctgacc gtcaccgaag tgaaaggttt cgggcgtcag aaagggcatg
    20821 ccgagctgta ccggggggcg gaatacagcg tcaacttcct gccaaaagta aaaattgatg
    20881 tggcgattgc tgatgaccaa ctcgatgaag tgatcgatat cgtcagtaag gcggcttaca
    20941 ccggaaaaat tggcgacggc aaaatcttcg tcgctgaatt gcaacgcgtc attcgtattc
    21001 gtaccggcga agccgacgaa gcggcgctgt aatctctggc acacagcaac aggaacgaaa
    21061 aatgaagata gcgacgataa aaactgggct tgcttcactg gcgatgctgc cgggactggt
    21121 aatggctgca cctgcggtgg ccgataaagc cgacaatgcg tttatgatga tttgtactgc
    21181 actggtgctg tttatgacta ttccggggat tgccctgttt tacggtgggt tgattcgcgg
    21241 caaaaacgtg ctgtcgatgc tgacgcaggt gacggtgacg tttgcactgg tctgtattct
    21301 ctgggtggtt tacggttact cgctggcgtt tggtgagggc aacaacttct tcggcaacat
    21361 taactggttg atgctgaaaa acatcgaact gacggcggtg atgggcagca tttatcagta
    21421 tatccacgta gcgtttcagg gatcgtttgc ctgcattacc gtaggcttga tagttggggc
    21481 gctggcggaa cgaatccgct tctcagctgt gttgattttc gtggtggtat ggctgacgct
    21541 ctcttacatt cctattgcgc atatggtgtg gggcggtggt ttgctggctt ctcacggtgc
    21601 gctggatttc gcgggtggca cggtggtgca tattaacgcc gcaatcgccg gtctggtggg
    21661 cgcgtatctg ataggcaaac gcgtgggctt cggtaaagag gcgtttaaac cgcacaacct
    21721 gccgatggtc ttcaccggga ctgccattct ctatatcggt tggtttggct ttaacgccgg
    21781 gtcagcgggc acggcgaatg aaatcgcggc actggcattt gtgaatactg tggtcgcaac
    21841 ggcggcggca attcttggct ggatcttcgg tgaatgggcg ctgcgtggta agccttcact
    21901 gctgggagcg tgttctggcg cgattgccgg tctggtcggc gtgacgccag cgtgtggcta
    21961 cattggtgtt ggcggcgcgt tgattatcgg cgtggtagct ggtctggcgg gcttgtgggg
    22021 cgttaccatg ctcaaacgct tgctgcgggt ggatgatccc tgcgatgtct tcggtgtgca
    22081 cggcgtttgt ggcattgtcg gctgtatcat gactgggatt tttgccgcca gctcgctggg
    22141 cggcgtgggc ttcgctgaag gtgtgacgat gggccatcag ttgctggtac agttggaaag
    22201 cattgccatt acgattgtgt ggtctggtgt ggtggcattt atcggctaca aactggcgga
    22261 tctgacggtt ggtctgcgtg tgccggaaga acaagagcga gaagggctgg acgtcaacag
    22321 tcacggcgag aatgcctata acgcataaca agcactgcaa aaaacagccg gacggttttc
    22381 acctccggct atttttttaa ttgtgattac gcatcacccc ttcctgaacg gtcgaggcaa
    22441 ccagtacgcc gtcctgggta taaaactcac cgcgcacaaa gccacgtgcg ctggacgccg
    22501 aggtgctctc cacgctatac agcagccatt cattcaaatt aaacgggcga tggaaccaca
    22561 tggaatggtc aatggtggca atctgaatcc ccggttcgag aaaaccgata ccgtgcggct
    22621 gtagagctac cggcaggaag ttaagatcag aagcgtaacc gagcagatac tgatgaacgc
    22681 gcaggtcatc cggcacgcta ccatttgcgc ggatccacac ctgacgatgt ggttctgcga
    22741 cgtgaccttt cagtgggtta tgaaactcca ccggacggac ttccagcgga cgatcgcaga
    22801 tgaatttatc tttaagcact ggcggcagca ggtgcgccag cgattgggcg atttgcgttt
    22861 ccgaaggcag gccatcaggc gctggcgcgg acggcattgt tttttgatgt tcgaaacccg
    22921 cttctggtgc ctggaaagag gccgtcatat aaaaaatcgg tttgccgttt tgaatagcag
    22981 caacccggcg ggcgctgaag ctgttaccgt cacgcagcgt ttcgacatca taaataatcg
    23041 gcttcttact atcgccaggg cgaagaaagt agctgtgaaa cgaatgtacc agccgctctt
    23101 cagggacggt ctcttttgca gcatacaagg cctgacccac gacctggccg ccaaatacct
    23161 ggcgtaaacc taaatcttca ctctggccgc gaaagagtcc ttcctcaatt ttttccagat
    23221 ttaacaatgt cagtaaattt tttagcgcct gactcatata actctccagt aacaaagctg
    23281 ccgcagcaag ccaaagtgag ttgagtataa cgcaaatttg ctactggtcc gatgggtgca
    23341 atggtctgaa ttacgggcta attacaggca gaaatgcgtg atgtgtgcca caattgttga
    23401 cgttactatt ttgttaacca ctcttccggc gaggaaagtt agcccgctgg tgcattgata
    23461 ataaggagaa atgaatgaaa ctcgtgcaca tggccagtgg tttagcggtt gcgattgcgt
    23521 tggcggcttg cgcagataaa agcgcggata ttcagacgcc agccccggct gcaaatacgt
    23581 ctatttcagc aacacaacaa cctgctatcc agcaaccgaa tgtctccggt accgtctgga
    23641 tccgtcagaa agtcgcactg ccgcctgatg ctgtgctgac cgtgacactt tctgacgcgt
    23701 cgttagccga tgcaccgtca aaagtgctgg cgcagaaagc ggtgcgtact gaaggtaaac
    23761 agtcaccatt cagctttgtt ctgccattta acccggcaga tgttcagccg aacgcgcgta
    23821 ttctgttgag tgcggcgatt accgtgaatg acaaactggt atttattacc gataccgttc
    23881 agccggtgat caaccagggg ggaaccaaag ccgacctgac attggttccg gttcagcaaa
    23941 ccgccgtgcc tgtccaggcc agtggtggcg caacaactac cgtaccatcg acttcaccga
    24001 cccaggtgaa tccatcttcg gcagttcctg cccctacgca atactaagcg ttactaaccc
    24061 ctttccatct ggagaggggt tagtagttcc agcgatagcg ctgtaagtcg atttgcccgc
    24121 ttcccgatac catcacacct tctgccagta atgcctgtcg ttgacgctgt aaatccggtc
    24181 cggttagcga aatggtgccg tggcgattaa ccacccggtg ccagggcaag gtgctgcctt
    24241 cggggagacg ctttaacaca ccgccaacct ggcgcgcggc gcggggcgat cccgccagtt
    24301 tcgccacatc accgtaagtg gtgacatacc cttcgggaat agcggctacg atttgccaga
    24361 cgcgttgggg aaatgaatct tctttttcca tcttttcttc ctgagataat ttttcagcat
    24421 aatctggaaa aacgcccgag tgaagccgca ttgcgcaaga aaccagcatc tggcacgcaa
    24481 tgggttgcaa ttagccgggg cagcagtgat aatgcgcctg cgcgttggtt ctcaacgctc
    24541 tcaatggggg ctctgttggt tctcccgcaa cgctactctg tttaccaggt caggtccgga
    24601 aggaagcagc caaggcagat gacgcgtgtg ccgggatgta gctggcaggg cccccaccca
    24661 tttctgcctc ccaccgtttc gtcaaaaaat cccaacatga ctaaacttta accacaactg
    24721 acgtcgcaag aattgtctgg ctgcgcagta cgcttcggag gtatgtctga tgaagtatgt
    24781 tgatggtttt gtggttgccg ttcctgccga taaaaaggat gcctatcggg aaatggccgc
    24841 taaggccgcg ccattgttta aagagtttgg cgcgcttcgt attgtcgaat gctgggccag
    24901 cgatgtaccg gatggcaaag tgaccgattt tcgtatggcg gtgaaagcgg aagagaatga
    24961 agaggttgtc tttagctgga ttgaataccc ttcaaaagag gtccgcgacg cagctaatca
    25021 aaagatgatg tcggacccac ggatgaaaga gttcggcgag tccatgccgt ttgacggcaa
    25081 gcgaatgatc tatggcggat tcgagtcaat catcgacgaa taggtagcgt gacgggctgt
    25141 gcggcgtgcc gtcagcccaa aaattcacaa atgttgctcg gcccagcgta agaaatcttc
    25201 tttcggtaat gccttgctgt agagccagcc ctgaccgtag tgcacgccat gctggcgtag
    25261 ccactcttct tgtttactgg tttcgattcc ctccgctacc attttcagtt tcagtgtttt
    25321 tgccatttcg atgatatgcg gcgtgacatt tttatattcc agcgcatcaa cgaaagattt
    25381 atcgatcttc agaatgtcga catccagatc ctgtaaataa cttaaacttg aataccccgt
    25441 accaaaatca tcaagataaa tttcatggcc cgcctcccgg tagcgagaaa ttatcggggc
    25501 gctggttttc ggatcggcaa actcgcgttc agtgagttca agcgcgatct gtctgggatt
    25561 aacctgatag tgattgatca tttcacgcag caattgcggg attttttccg aggtgagcac
    25621 ggtggattca agattgatcg aaatatgctg ctgtggatgc tgacgcagcc agtcgcccat
    25681 atcttcaaag acgcttctta taatcagtag cgtcaatggc tcagaaaggc ccgtttgctg
    25741 tgccagcgga ataaaactat ctggtgacaa ccaactaccg tctgtctgcg gccagcgcgc
    25801 cagtgcctca gcaccgacaa ttttgccatt ggctaaggag acaatcggct gatagtgcac
    25861 gcaaatatca cgattttcga tagcatcctg cagccgatga tgcggtgact ggatgcggcg
    25921 caaaatgcgc agcacaaaca tcgctgccag caggccaatc accaacccga gcggtaacca
    25981 aataaagact tgccgatgcc agcctttctg caacattttc gatgaagccc aggtgataat
    26041 tgaaatattc atctccggga agggcaggat atcgtaaatg attccattat tttcgatatg
    26101 ctcaccgggt gttttttgta gcctggtaat aattccctga gcaatttcat cgctgctggt
    26161 tatgacaacg ttatgggcat tgccaataat ggcggcatca atttgccatg agctataggg
    26221 aatgacatca ataaaggaag cggggtcgat catgacgaca taatgtgccg ttcccatggc
    26281 gaccatgtaa cggataatgc ctaaatcgtt atgtgatgtt aaccagacac gataaccatc
    26341 tttcgaaatt ttacctggct cggggaaggt atcgggcgga ctttcatgct ccagagacga
    26401 acactgggga acgttgttat cgatataaac cacttcctga atatagcgat aactgtaaga
    26461 tacccgacgc atttccatga gatgggcttc actacagtca gcgccttgcc atctttccag
    26521 ctcttgcagc gcatctttcc cttgtgtcgc caccttattg gcccgaatag cgacgcggga
    26581 ggaataggta tccagctctt caataaacga tgtttctacc tgctgatggg ccagccagat
    26641 gcttaagccg acaggcagca atactgaaag aatcagtact cccgaaataa ggccgaccag
    26701 atgtcgtgtt ctcaccagca tatccttatt aacacagtgt agggaaagta tatctgattg
    26761 tggaaaaagg ctggcaggag ataaggaggt agagctggta tttcatgaag tgatgggtgg
    26821 aaacgatcgt tgtcatttga ccaggcgcag agctttacgc ctggtatgta atcctaactt
    26881 aagatgttga ttcggcgcga gcgagggtaa aaatatcgta aaaagacaat tcacctttac
    26941 ggacgatcat tttttgcagt tgttccttat gaacatccgc gacacgcgga gagttcaaga
    27001 tggcttcaac cagcgcggca ggtacaaagc gcgtgttgta ccattcgccg ttatagcaaa
    27061 cgcggaaatc caacacgtcg atatcttcat agcggtagcg tgggtagaca tcgaaaaaga
    27121 agaaaccatt aagcaggata aataatacaa ccagtagcca gacagagcct gaaagcgttt
    27181 cggactgcaa catcaccgcc agggtggcaa aaaaagcaac gtacataccg ataaacagcc
    27241 ccggatgttt acggataaaa ctgatactaa agcggggttt gttgtcgcgt ttttcgcggg
    27301 tattcagaga ctcaatcgtt tcggtcagca ggcgttgtat ttcggtcatc gtttgccttc
    27361 gtggtatctg caactttcca gggaacaccc tattttaacg tgggaatagt agagggaaag
    27421 taacagatct gccacaattt tgcataacag ttgcgaaaaa ccgattacaa ttttttaatt
    27481 attctggctg gattaccgcc cacgacaacg ttgtccggga catcttttgt gacaactgca
    27541 cctgaggcta ccacgacgtt atcaccaatg gtcacaccag ggttaatgac cgcgcgtccg
    27601 ccaatccaga cgttattacc gatggtgacg ggtttcccca gttcagcacc gctattacgt
    27661 gctacagggt cgatgggatg tgttgccgtg tagatatgaa cgcctagtgc caacatacag
    27721 ttatcaccga tgcgaatagg gcagacatca agcatcacgc aatcgaagtt ggcgaaaaaa
    27781 ttattaccga gaaaaatgtt atagccatag tcacagcgaa acgttggctc aatataagcc
    27841 tctgtcacct gaccgaatag atcagcgaga atttgctggc gtaatgtgtg ctcttccgcc
    27901 agggaatgat tgtatcggtg aataagctga cgagcgcgca ggcgatcgcg agataacgtc
    27961 tcatctgccg agcgatacaa ctcaccagca atcatctttt ctttttctgt gctcattact
    28021 gacctccggt tgctgaaaat gcggcaacac agtaagacga aaagtcagtg aagagaacgt
    28081 tcccattaca tctgtgtgat aaagatcaca tagggtatgt cttcatggcg aaaaagtata
    28141 aaattcttaa taaacagccg gttatagctc cgaaagcgaa ttatttatta gcgaataaat
    28201 ttccatactg aggaagggat cttgtcgtac agtttattca tggtcaattc ggcgaggcgg
    28261 tgatctgcgg ctgagtaaaa taccgccagt tcattatctg ataattcgta tttatttttc
    28321 tcgataacac gctccagcgt gtcaattgtc tggcaacgac gtaaacgcat taaataatcg
    28381 gttttcgtta aaggtttttc ggacatactt ctacccatgg ttgtaataat tctaacaaga
    28441 taaactcgca ggattctctt tcgtcgcatt gacaaaacaa cggaatagtc gattacctga
    28501 cttccgccat ttctgaagat cctgcatatt aataccataa ctactgaaca acataaaggt
    28561 gtcatccaga tattcgtcga tctgctcaat gagcttattg tcttcattat acttaatttt
    28621 gtaattaagt gcgaaggtcg caatatgctc aatcagttca ttcaactgga ggttgatcgc
    28681 cgaggttggg tcgttaaccc agccatgatt gctttcttca aggtttgcaa ggcagtcatg
    28741 atacagagtt tcacagagaa acttaagctg tgcgatatca tgtcttttgg gtgagtattc
    28801 atccataacg cgtccccttc ttagcggttg aactaacgga cacctttcgg gatggaaaaa
    28861 acttactgac ctggacttgc cctctcgttt gttagcttaa ctataagcca ctctttgcag
    28921 gttttcatcg cattattacg aaaaattaca attaggaagg aagcgggaca gggctcactt
    28981 tttcatacta gcaccaaccc gcagcaggta aaagcagttt gctcgaactg ttcataacat
    29041 ttcaggatta tcgccagatg aaattggtga cactaatacc aggattgctc tgaatatgac
    29101 gtaataaccg aggaatgaat aaagaattac cgcaaataat taagaatagc ctctaaatga
    29161 ttatggatta ttaaatctat tagcgctact taatataaat taactaacaa tcagtatttt
    29221 ttatgaattt tatacgtggt taatactggt tttcgtatga gatcctgagt tggtggttca
    29281 attactcctt aatgttcgta ggttatgcat aaaaaaggcc gcttacgcgg ccttagtgat
    29341 tacacgttgt atcaatgatg atcgacagta tggttgtgct cgatatcttc attcttgcgg
    29401 ctaaagcggc ggcgaaccac cacaaagaat accggaacga agaagattgc cagtaccgtt
    29461 gcggtcacca tcccgcccat tacaccggta cctactgcgt tctgcgcgcc ggaaccagca
    29521 ccagtactga taaccagcgg cataacgccg aggataaacg ccagcgaggt catcaggatc
    29581 ggacgtaaac gcatccgcac cgcatcaagc gtcgcttcaa tcagaccttt accttcttta
    29641 tccatcaagt ctttggcgaa ttcgacgata agtatcgcgt tcttcgccga caacccaatg
    29701 gttgtgagca ggcctacctg gaagtaaacg tcattggtca ggccacggaa ggtggcagcc
    29761 agcaacgcac cgataacccc cagcggaacg accagcataa cggagaacgg aatcgaccag
    29821 ctctcgtaca gcgccgccag acacaggaac acgacaatca acgaaatcgc gtacagtgaa
    29881 ggtgcctggt tgccggagag acgttcctga taggacatcc ccgtccagtc atagccaaca
    29941 ccggtaggca gtttgctcgc cagttgttcc atcagctcca ttgcttcacc ggtactttta
    30001 cccggtgccg cctggcctaa gatttccatg gatggcaggc cgttgtaacg ttccagacgc
    30061 ggcgaaccgt actcccaacg agaagaggag aacgccgaga atggcaccat ctgaccatca
    30121 gcagcacgaa cataccagtc gccgatatca tccggcagca tacggtattt cgcttctgac
    30181 atgacgtaaa ctttcttcac acgaccgcgg tcgataaagt cgttcacata gctgccgccc
    30241 catgcagcgc ccagagtggt gttaatgtcg ttgatagaaa cacccagcgc ctgcgctttt
    30301 tcctggtcga tatcaatctt aaactgcggg gtatcttcca gaccgtttgg acgtacgctg
    30361 gtcaacatat cagggtgctt cgctgcttct gcaagcaact ggttacgcgc ctgagtcagt
    30421 ttttcgtgac caaggccagc ctggtcaatc agctcaaagt caaagccggt tgcggtaccc
    30481 agttccacga ttgcgggcag gttaaaggcg aaaaccatcg catctttgat ttgcgagaaa
    30541 gcgcgtgttg cacgcatggt aatcgcttca actttgtttt cttcacccgg acgatcggcc
    30601 cagtccttca aggaaacgaa cgcaataccg gtattctgac cacgtcccgc aaagccgaag
    30661 ccgttaacgg cgaacaccga ctcaacgttg ttcttttctt tggtcagata gtaatgcgtt
    30721 acctcattga gcactttctg cgtacgttcc tgcgttgcac ctgctggcag ctgaaccatg
    30781 gtcataaata cgccctggtc ctcatctggc aagaaggagc ttggcagacg cacaaacaga
    30841 taggccatgc cgaccacgat gatcagatac agcaccagat aacgccccgt actgcgcaga
    30901 ataccgccta cgctgtcggt gtagtggtgc gtgctcttct cgaacatgcg gttaaaccag
    30961 ccgaagaagc cttttttacc ttccccgtga tcgcctttgg caatcggttt cagcatggtg
    31021 gcacaaagag ctggagtcag gatcaacgcc accagtaccg acagcgccat tgctgaaaca
    31081 atggtaatag agaactgacg ataaattgcc ccagtagaac cgccaaagaa ggccatcggt
    31141 acgaataccg ccgacagtac catcgcgata ccgaccagag cgccctgaat ctgccccatc
    31201 gacttacggg tggcttcttt tggcggcaaa ccttcttccg ccatcacacg ctcaacgttt
    31261 tctaccacaa cgatagcgtc atccaccaac aggccgatgg cgagcaccat cccgaacatt
    31321 gttagcgtgt ttatcgagaa gccaaaggcg gcaaggacgg caaaggtccc gagcaatacc
    31381 accggtacgg caatggtcgg aatcaacgtc gcgcggaagt tctgcaggaa cagatacata
    31441 accaggaaca cgaggatgat cgcttcgacc agcgttttaa ccacttcgtg aatagagatt
    31501 ttcacgaacg gcgtggtgtc gtacgggtaa acaattttca gacccgacgg gaagaacggt
    31561 tccatcttcg ccagttcagc acggattgcc gcagcggtat ccagcgcgtt tgcaccggtc
    31621 gccagcttga tccccagacc ggaagccggt tggccgttaa actctgcgat gatgtcgtag
    31681 ttctcaccac ccagctcaat cttcgccaca tcacgcagca gtacgcggga accatcctga
    31741 ttcactttca gcaggatttt gccgaactct tcagtagagg tcagacgcgt ctgagcaata
    31801 atagaggcgt taagctgttg gcctttcacc ggcggtgtac caccgagctg gcccgccgca
    31861 acctgggcgt tctgcgcttt gatggcggta ataacatcaa ccggcgttag ctggaatttg
    31921 ttcagttcat tcgggttcat ccagatacgc atcgcgtact gtgaaccgaa caactgaacg
    31981 tcacccacgc cagacgtacg gctgatggca tctttcatat tcgccgccac gtagtcggag
    32041 atatcctcct gcgtcatggt gccatcggtg ttgataacgc cgacaaccat caggaagctg
    32101 ctggatgatt tctcaatgct caccccttgc tgctgaactt cttgcggcag caacggcatc
    32161 gccagctgca gtttgttctg cacctgaacc tgcgcgatat ccgcatcagt accagactca
    32221 aaggtcaggg tgatctgtac ggtacccgtg gagtcactgt tagaggacat gtacatcagg
    32281 ttatcgatac cgttcatatt ctgttcgata acctgtgtca ccgtgtcctg cactgttttc
    32341 gcatcagcgc cggggtagga ggcggagatc gttactgccg gcggtgcaat cgtaggatat
    32401 tgcgccaccg gcagtttgag gatcgccagc ccccctgcca acatgatgat aatggcgatc
    32461 acccacgcaa aaatcgggcg atcgataaag aaattaggca tgtcttaacg gctcctgttt
    32521 aagttaagac ttggactgtt caggctgagc accgcttgcg gcttgctggt tattatcagc
    32581 ggtaacttct tgtgctttta cctggacacc aggacgcact ttctgcagcc cacttattac
    32641 tacgcgatcg cctgctttca gaccttctgt caccagccac ttatcgccaa tagcctggct
    32701 tgcaacgatc ggacgggttt ccactttgtc atccgcgcca accaccagta cggtggcatc
    32761 gccacgcggc gtacgggtta cgccctgttg cgggactaaa atagcgtttg gattaagccc
    32821 ttcttccaga cgtgcgcgca cgaacatacc cggcagcaga gtgtgatccg ggttcgggaa
    32881 gatagcgcgt agggtgatag acccagtggt ctgatcaacg gtaacgtcag agaattccag
    32941 cgtaccgtcc tgcgggaact taatgccgtc actggtgatc agcgacactt tggctttgcc
    33001 gttctcttgt ttcagcgtgc cattcgccag ttcctgtttc aggcgcagga agtcgttgct
    33061 ggactgggtc acatcaacgt agatcggatc aagttgctgc acggttgcca gcgcagtcgc
    33121 ctgaccgttc tgtaccaatg cgccttccgt cacgttcgac ttaccaatgc gaccgctaat
    33181 cggagaggtg actttggtgt aagccagatt gatccgcgca gtttcaacgg cagctttcgc
    33241 cgcagttacc gcagcattcg cctgttgcgc atcagccaga gcctgatcgt actcttgctt
    33301 actgatgtac tgagtaccga gcagtttctg ataacgattc accgtcaatt gcgcgatatt
    33361 ggctgcagcc tgggctttcg ccagatcacc tttcgcactg tcgtatgtcg cctgataggt
    33421 cgcaggatca atctgataga gagagacacc tgcttcgatg tcgctacctt ctttgaaatt
    33481 acgcttcagg ataatcccgc taacttgagg acgaacttct gcgatccggt aggcactggt
    33541 gcgacccgga agctcggttg tgatctgcag aggttcagtt ttgactgtta ctacgccaac
    33601 ggcgggcatc tgctggccac cttgttgggc ctgtttgtcg tcacatcctg ttagggctaa
    33661 gctgcctgag agcatcagaa cgaccgccag aggcgtaaac cctctgtttt tgttcatatg
    33721 taaacctcga gtgtccgatt tcaaattggt caatggtcaa aagttaataa acccattgct
    33781 gcgtttatat tatcgtcgtg ctatggtaca tacattcaca aatgtatgta aatctaacgc
    33841 ctgtaaattc acgaacatat ggcacgaaaa accaaacaag aagcgcaaga aacgcgccaa
    33901 cacatcctcg atgtggctct acgtcttttc tcacagcagg gggtatcatc cacctcgctg
    33961 ggtgagattg caaaagcagc tggcgttacg cgcggtgcaa tctactggca ttttaaagac
    34021 aagtcggatt tgttcagtga gatctgggaa ctatcagaat ccaatattgg tgaactagag
    34081 cttgagtatc aggcaaaatt ccctggcgat ccactctcag tattaagaga gatattaatt
    34141 catgttcttg aatccacggt gacagaagaa cggcgtcgat tattgatgga gattatattc
    34201 cacaaatgcg aatttgtcgg agaaatggct gttgtgcaac aggcacaacg taatctctgt
    34261 ttggaaagtt atgaccgtat agaacaaacg ttaaaacatt gtattgaagc gaaaatgttg
    34321 cctgcggatt taatgacgcg tcgggcagca attattatgc gcggctatat ttccggcctg
    34381 atggaaaact ggctctttgc cccgcaatct tttgatctta aaaaagaagc ccgcgattac
    34441 gtcgccatct tactggagat gtatctcctg tgccccacgc ttcgtaatcc tgccactaac
    34501 gaataaccct gaatctgact ccaggatttt tcctggacat tttcgtcgtt gctattctgg
    34561 ttcactgcgt cgtgatattc ttgcggtttg actttttcag gtcgttcttc aggttcagaa
    34621 accttcattc atcatgacta tgttccagta ttacaaacga tcacggcatt ttgttttttc
    34681 agcatttatt gcttttgttt ttgtcttgtt atgccagaac acggcgtttg cgcgggcgtc
    34741 atcgaatggt gatctgccga caaaagcgga cctgcaggcg caacttgact cactaaataa
    34801 acaaaaagat ctttctgctc aggacaaact ggtgcagcag gatctgacag atacattagc
    34861 caccctcgat aaaatcgatc gcgtaaaaga agagaccgtt cagctacggc aaaaagtcgc
    34921 tgaagcgccg gaaaaaatgc gccaggcgac cgcggcgtta acagcactta gcgatgtcga
    34981 taacgacgaa gaaacgcgca aaattctgag cacgctttcg ttgcgccagc tggaaactcg
    35041 cgttgcccag gcgctggacg atttgcaaaa cgcacaaaac gatctggcgt cttataacag
    35101 ccagctggtt tcgttacaga cgcagcccga acgcgtgcaa aatgcgatgt ataacgcttc
    35161 gcagcagctg caacaaattc gcagtcgtct ggatgggact gatgtcggcg agacggcctt
    35221 acgtcccagc cagaaagtgt taatgcaggc tcagcaggcg ttgctgaatg cggagattga
    35281 ccagcagcgt aaaagcctgg aagggaatac cgtcttgcag gataccttgc aaaagcaacg
    35341 tgattacgtg acggcgaaca gcgcccgtct ggagcaccag ttgcagctgt tgcaagaagc
    35401 ggtaaacagc aagcgcctga ctttaaccga aaaaacggcg caggaagccg tctccccgga
    35461 tgaagccgcg cgtattcagg ctaatccgct ggtgaagcag gaactggaaa ttaaccagca
    35521 gttaagtcag cgtctgatta ccgcgactga aaacggtaat cagttgatgc agcaaaacat
    35581 taaagtcaaa aactggctgg agcgggcgct gcaatcggaa cgcaatatta aagagcagat
    35641 tgccgtcctg aagggcagcc tgctgttgtc tcgtatcctt taccagcaac aacaaacgct
    35701 gccctcggcg gatgaactgg aaaacatgac caaccgcatc gcggatttgc gtctcgaaca
    35761 gtttgaagtt aaccagcagc gtgatgcact cttccagagc gatgcgttcg tcaacaaact
    35821 ggaagaaggt cacaccaacg aagtcaacag cgaagttcac gatgcgttat tgcaagtggt
    35881 tgatatgcgt cgcgaattgc tggatcaact caacaaacag ttgggtaacc agctgatgat
    35941 ggccattaac ctgcaaatca accagcagca gttaatgagt gtgtcgaaaa acctgaaatc
    36001 catcctgact cagcaaatct tttgggtgaa cagtaaccgt ccaatggact gggactggat
    36061 caaagcgttc ccgcaaagcc tgaaagatga atttaagtcg atgaaaatta cggttaactg
    36121 ggaaaaagcc tggcccgccg tttttatcgc tttcctcgct ggtttgccgc tgctgttgat
    36181 tgccgggctg atccactggc gtctggggtg gctgaaagcg tatcaacaaa aactggcttc
    36241 cgctgtgggt tccctgcgta acgacagcca gctcaacaca ccaaaagcga tccttatcga
    36301 cctgatccgt gcactgccgg tgtgcctgat tattctcgcg gttggcctga ttctgttgac
    36361 catgcagctc aacatcagcg aactgctatg gtcgttcagc aaaaaactgg cgattttctg
    36421 gctggtgttt ggcctgtgct ggaaagtgct ggagaagaac ggcgttgccg tgcgtcactt
    36481 cggcatgccg gaacagcaga ccagccactg gcgtcggcaa attgtccgca tcagtctcgc
    36541 gttgctgcct atccatttct ggtctgtggt ggcagaactt tccccgctgc atctgatgga
    36601 tgatgtgctg gggcaggcga tgattttctt caacctgctg ctgattgcct tcctggtatg
    36661 gccaatgtgc cgcgaaagct ggcgtgataa agagtcgcac accatgcgac tggtcaccat
    36721 taccgtgctg tcgattatcc cgattgcatt gatggtgctg actgccacgg gttatttcta
    36781 caccacgctg cgcctctccg ggcgttggat tgaaaccgtt tatctggtga tcatctggaa
    36841 cttgctctac cagacggtat tgcgcggctt aagcgtagcg gcgcggcgta tcgcctggcg
    36901 tcgtgcgctg gcgcgtcggc agaatctggt gaaagagggc gcagaaggtg ccgaaccgcc
    36961 ggaagaaccc accattgcac tggaacaggt taaccagcag acgctgcgta ttaccatgtt
    37021 gctgatgttt gcgctgttcg gtgtcatgtt ctgggcaatt tggtccgatt tgatcaccgt
    37081 gttcagctat ctcgacagca ttacgctgtg gcattacaac ggtactgaag ctggtgctgc
    37141 ggtggtgaaa aacgtcacca tgggtagtct gttgtttgcg attatcgcct caatggtggc
    37201 ctgggcgttg attcgcaacc tgcctggttt gctggaagtg ctggtgctct cgcgactgaa
    37261 tatgcgccag ggcgcgtcgt atgccattac taccatcctt aactacatca ttattgctgt
    37321 tggtgcgatg acggtgttcg gatcgctggg cgtctcttgg gataaactcc agtggctggc
    37381 cgcagcatta tccgtaggtc ttggttttgg tttacaagaa attttcggta acttcgtctc
    37441 cggtttgatc attctgttcg aacgtccggt gcgtattggc gatacggtaa ccattggtag
    37501 cttctcgggg acggtaagta aaatccgtat tcgtgcgaca acgattaccg atttcgatcg
    37561 caaagaagtg atcatcccga acaaagcgtt tgttaccgag cgtctgatca actggtcgtt
    37621 gactgacact actacgcgtc tggtgatccg tctcggcgtg gcctatggct ccgatctgga
    37681 aaaagtgcgt aaagtgttac tgaaggcggc gactgagcac ccaagggtga tgcacgaacc
    37741 aatgccggaa gtcttcttta cggcatttgg tgccagcacg ttggatcatg agctgcgtct
    37801 gtatgtgcgt gaactgcgtg accgtagtcg tactgtcgat gagctgaacc gtactatcga
    37861 tcagctgtgc cgtgaaaacg acatcaacat tgcctttaac cagcttgaag tgcatctgca
    37921 caacgagaag ggcgatgagg tgacggaagt aaaacgcgac tacaaaggcg acgatccgac
    37981 gccagcggta gggtaaggac aataaagggc gacagtgatg ccgcccttta ttttttactc
    38041 gctttgtgaa gcctcatcgc tctgtaactt cttttcataa tcacgcttta caatatccag
    38101 cgcgcgcagc acggtgcggg ctgggatctg attttcttcc agcagcacaa tcaaatcgac
    38161 agccagtttg acatcgtcag gggcattttc cagtgacata ctctctccat tgctagcggg
    38221 ttaaacgcgc taacctgttt tcgatttttt ccagcgcatg gcggcagcgt gccaggcgcg
    38281 cttcataggc ttccacttca cgatgcagcg tttgctgttc cacgagatcg gtcacccgcg
    38341 ccaggcgggc tctacgttcg gcaaccatct cacgtagccg ccgctcaaaa tcctgatgct
    38401 gaatacgttt acgctgccag cgggcaattt ggggtggtgc gctatcccac tcgcgtagcg
    38461 accaggctgt ggcttcacgc gctatagcct ccagttgcgc cgccagatgt tctgccagcc
    38521 aggcgacttg cggcagttgc tgttgctcaa ctgcatgacg aagcgcagcc agattatcgc
    38581 ccgccttgtc gagacaagcc tgtagtgttg tcgcacgagt ctgaaaaaga tgcctgttga
    38641 aacgagcact tagcgtggcg aactgtgaca ccggggcaca acgctgacgc agcgtagcga
    38701 gctgtccttc cagtttttcc agcagcaggg cggttttcac gataagacct caatgaaaat
    38761 gataattgtt atgctaaagt agccactctg taagctgaca acactcaata tgcaacgaat
    38821 cattttaatc atcattggct ggctggcggt agtgctgggc acgctgggcg tggtattacc
    38881 ggtattaccg acgacgccgt ttatcctgct ggcggcctgg tgctttgccc gttcttcccc
    38941 gcgctttcac gcctggttgc tgtaccgctc atggtttggc agctatctac gtttctggca
    39001 gaaacatcat gcgatgccgc gcggcgtcaa accgcgggcg attttgctta ttttgctcac
    39061 gtttgccatt tctctgtggt tcgtccagat gccatgggtg cgcatcatgt tgctggtaat
    39121 tctcgcctgt ttgcttttct atatgtggcg aattccggtg attgatgaaa agcaagaaaa
    39181 gcactgaagc acaacaatcg cagttgcaat tattgcgtac agccagtaca ttctggcgtt
    39241 ttcgagcaca ggcgcaggcg gtcaaaggtt aaacaactgt tacttttgat acgtttaaaa
    39301 cgcgccgtga gtaccaccgt aacaagcagg catacactta tgaccgcgac tgcacagcag
    39361 cttgagtatc tcaaaaatag catcaaaagc attcaggact acccaaaacc cggcattctt
    39421 ttccgcgatg tcaccagctt actggaagac ccgaaagctt acgctctcag catcgacttg
    39481 ctggttgagc gttacaaaaa tgcgggcatt aacaaagttg tcggcaccga agcgcgtggc
    39541 ttcttgtttg gcgctccggt agctctgggt ctgggcgttg gctttgtacc ggtccgtaaa
    39601 ccgggcaaac tgccgcgtga aaccatcagt gaaacttacg atctggaata cggcaccgat
    39661 cagctggaga tccacgttga tgccatcaaa ccgggcgaca aagttctggt ggtggacgat
    39721 ctgctggcaa ccggcggcac tatcgaagcg accgttaaac tgatccgtcg tctgggtggt
    39781 gaagtggctg acgctgcgtt cattatcaac ctgttcgatc tcggcggcga acagcgtctc
    39841 gaaaaacagg gcattaccag ctacagcctt gtcccgttcc cgggccatta attatcgcca
    39901 gtcttgtgct gcccacgcta cggacagcac aagatgtgca ttcagcctcg ccgttctgac
    39961 ggggctgtgt tagcattacc ccttcgagaa tccaccttcc agcgtttcag agcctgccaa
    40021 tgagttatca ggtcttagcc cgaaaatggc gcccacaaac ctttgctgac gtcgtcggcc
    40081 aggaacatgt gctgaccgca ctggcgaacg gcttgtcgtt agggcgtatt catcatgctt
    40141 atcttttttc cggcacccgt ggcgtcggaa aaacctctat cgcccgactg ctggcgaagg
    40201 ggctaaactg cgaaaccggc attaccgcga cgccgtgcgg cgtgtgcgat aactgtcgtg
    40261 aaatcgagca ggggcgcttt gtcgatctga ttgaaatcga cgccgcctcg cgcaccaaag
    40321 ttgaagatac ccgcgacctg ctggataacg tccagtacgc tccggcgcgt ggtcgtttca
    40381 aagtttatct catcgacgaa gtgcatatgc tgtcgcgcca cagctttaac gcactgttaa
    40441 aaacccttga agagccgccg gagcacgtta agtttctgct ggcgacgacc gatccacaga
    40501 aattgccggt gacgattttg tcgcgctgtt tgcaatttca tctcaaggcg ctggatgtcg
    40561 agcaaattcg tcatcaactt gagcacatcc tcaatgaaga acatatcgct cacgagccgc
    40621 gggcgctgca attgctggcg cgcgccgctg aaggcagcct gcgagatgcc ttaagtctga
    40681 ccgaccaggc gattgccagc ggtgacggcc aggtttcaac ccaggcggtc agtgcgatgc
    40741 tgggtacgct tgacgacgat caggcgctgt cgctggttga agcgatggtc gaggccaacg
    40801 gcgagcgcgt aatggcgctg attaatgaag ccgctgcccg tggtatcgag tgggaagcgt
    40861 tgctggtgga aatgctcggc ctgttgcatc gtattgcgat ggtacaactt tcgcctgctg
    40921 cacttggcaa cgacatggcc gccatcgagc tgcggatgcg tgaactggcg cgcaccatac
    40981 cgccgacgga tattcagctt tactatcaga cgctgttgat tggtcgcaaa gaattaccgt
    41041 atgcgccgga ccgccgcatg ggcgttgaga tgacgctgct gcgcgcgctg gcgttccatc
    41101 cgcgtatgcc gctgcctgag ccagaagtgc cacgccagtc ctttgcaccc gtcgcgccaa
    41161 cggcagtaat gacgccaacc caggtgccgc cgcaaccgca atcagcgccg cagcaggcac
    41221 cgactgtacc gctcccggaa accaccagcc aggtgctggc ggcgcgccag cagttgcagc
    41281 gcgtgcaggg agcaaccaaa gcaaaaaaga gtgaaccggc agccgctacc cgcgcgcggc
    41341 cggtgaataa cgctgcgctg gaaagactgg cttcggtcac cgatcgcgtt caggcgcgtc
    41401 cggtgccatc ggcgctggaa aaagcgccag ctaaaaaaga agcgtatcgc tggaaggcga
    41461 ccactccagt gatgcagcaa aaagaagtgg tcgccacacc gaaggcgctg aaaaaagcgc
    41521 tggaacatga aaaaacgccg gaactggcgg cgaagctggc ggcagaagcc attgagcgcg
    41581 acgcgtgggc ggctcaggtg agtcaacttt cgctaccaaa actggtcgaa caggtggcgt
    41641 taaatgcctg gaaagaggag agcgacaacg cagtatgtct gcatttgcgc tcctctcagc
    41701 ggcatttgaa caaccgcggt gcacagcaaa aactggctga agcgttgagc atgttaaaag
    41761 gttcaacggt tgaactgact atagttgaag atgataatcc cgcggtgcgt acgccgctgg
    41821 agtggcgtca ggcgatatac gaagaaaaac ttgcgcaggc gcgcgagtcc attattgcgg
    41881 ataataatat tcagaccctg cgtcggttct tcgatgcgga gctggatgaa gacagtatcc
    41941 gccccatttg atcgtaagca cagcttacgt tcgtcatcct taacgtgatt gagagagaaa
    42001 cctatgtttg gtaaaggcgg tctgggtaac ctgatgaagc aagcccagca gatgcaagaa
    42061 aaaatgcaga aaatgcagga agagatcgcg cagctggaag ttaccggcga atctggcgca
    42121 ggtctggtaa aagtgaccat caacggtgca cataactgcc gtcgcgtaga gatcgacccg
    42181 agcctgctgg aagacgacaa agagatgctg gaagacctgg tggctgcagc attcaacgac
    42241 gcagcacgtc gtattgaaga aacgcagaaa gaaaaaatgg cttctgtatc ctccggaatg
    42301 cagctgccgc ctggctttaa gatgccgttc tgatgcaaac cagcccgctg ttaacacagc
    42361 ttatggaagc actgcgctgt ctgccgggcg ttggcccgaa gtcggcgcag cgtatggcgt
    42421 tcacgctgct tcagcgcgat cgtagcggcg ggatgcgtct ggcgcaggcg ctcacccggg
    42481 cgatgtcgga aatcggccac tgcgccgatt gccgcacctt caccgaacag gaagtctgta
    42541 acatctgttc gaatccgcgt cgtcaggaaa acggtcaaat ctgcgtggtg gagagtccgg
    42601 cggacattta cgccattgag cagacggggc agttttcagg tcgttatttt gtgttgatgg
    42661 gacatctgtc accgctggac ggcatcggtc cggatgatat cggccttgat cgtctggaac
    42721 agcgtctggc agaggaaaaa atcactgaag tgatcctcgc caccaaccct acggttgaag
    42781 gtgaagctac cgctaactac attgccgagc tttgcgcgca atatgacgtg gaagccagcc
    42841 gaatcgctca tggcgtaccg gttggcggcg aactggaaat ggtcgatggc accacgctgt
    42901 cacactccct tgccgggcgt cataagattc gtttttaagc aaacgagagc aggatcacct
    42961 gctctcgctt gaaattattc tcccttgtcc ccatctctcc ctcatcctgt ttttaacctt
    43021 aaaatggcat tattgaggta gacctacatg aaaggacaag aaactcgtgg ttttcagtca
    43081 gaagtgaaac agcttctgca cctgatgatc cattctctct attccaataa agaaatcttc
    43141 ctgcgtgagc ttatctctaa cgcctccgat gcggcggaca agttgcgttt ccgtgcgctc
    43201 tctaacccgg acctgtacga aggtgatggc gaactgcgcg ttcgtgtctc tttcgataaa
    43261 gacaagcgca cgctgaccat ctccgataac ggcgtgggga tgacccgcga cgaagtgatt
    43321 gaccatctgg ggactatcgc taaatccggt accaaatcat tcctcgaatc cctgggttct
    43381 gaccaggcga aagacagcca gctgatcggt cagtttggtg ttggtttcta ctctgcgttt
    43441 atcgtggccg acaaagtgac cgtgcgtact cgcgcggcag gcgaaaaacc agaaaacggc
    43501 gtcttctggg aatcggctgg cgaaggtgaa tataccgttg ccgacatcac caaagaagat
    43561 cgtggtactg aaatcaccct gcatctgcgt gaaggcgaag acgagttcct cgatgactgg
    43621 cgcgtgcgtt ccatcatcag caaatactcc gaccatatcg cgctgccggt agagatcgaa
    43681 aaacgcgaag agaaagacgg cgaaaccgtt atctcctggg agaaaattaa caaagcgcag
    43741 gcgctgtgga ctcgtaacaa gtcggaaatc accgatgaag agtacaaaga gttctacaaa
    43801 cacatcgccc acgattttaa cgatccgctg acctggagcc acaaccgtgt tgaaggtaag
    43861 caggagtaca ccagcctgct gtacattccg tcccaggctc cgtgggatat gtggaaccgc
    43921 gatcataaac acggcctgaa actgtatgtt cagcgtgtgt tcatcatgga cgacgcagaa
    43981 cagttcatgc cgaactatct gcgcttcgtg cgtggtctga ttgactccag cgatctgccg
    44041 ctgaacgttt cccgtgaaat cctccaggac agcacggtaa cgcgtaacct gcgcaatgcg
    44101 ctgaccaagc gtgtgctgca aatgctggaa aaactggcga aagacgacgc ggaaaaatac
    44161 cagaccttct ggcaacagtt tggcctggta ctgaaagaag gtccggcgga agatttcgct
    44221 aaccaggaag cgatcgccaa attgctgcgt tttgcttcta cccataccga ttcttctgcg
    44281 cagaccgtgt ctctggaaga ctacgtttcc cgcatgaaag aagggcagga gaaaatctac
    44341 tacatcactg cagacagcta tgcggcagcg aagagcagcc cgcacctgga actgctgcgt
    44401 aagaaaggca tcgaagttct gctgctttcc gaccgcatcg atgagtggat gatgaactat
    44461 ctgactgagt tcgacggtaa accgttccag tcggtgtcta aagttgacga gtcgctggaa
    44521 aaactggctg acgaagttga tgagagcgcg aaagaagcgg agaaagcact gactccgttc
    44581 atcgaccgtg tgaaagccct gctcggcgag cgcgtgaaag atgtccgtct gactcaccgt
    44641 ctgaccgata cgccagcgat cgtttcgacc gacgcggacg aaatgagcac tcagatggcg
    44701 aaactgttcg ctgcggcggg ccagaaagtg ccagaagtga aatacatctt cgaactgaac
    44761 ccggatcacg tactggtgaa acgtgcggca gatactgaag atgaagcgaa gttcagcgag
    44821 tgggtagaac tgctgctgga tcaggcactg ctggcagaac gcggcacgct ggaagatccg
    44881 aacctgttta ttcgtcgtat gaaccagctg ctggtttcct gatgtaatgc cggatgacct
    44941 tcgtgtcatc cggcattttt cttttcatta tctgcacttt ccgcaaatta tctcgccatt
    45001 aaccgtttca gcccctggtg cctttcttga ggcaatcgcc tgatggtggt atcgtttatc
    45061 gctttttcaa aaaattcgac acattttaag gggattttcg caatgcgtat cattctgctt
    45121 ggcgctccgg gcgcggggaa agggactcag gctcagttca tcatggagaa atatggtatt
    45181 ccgcaaatct ccactggcga tatgctgcgt gctgcggtca aatctggctc cgagctgggt
    45241 aaacaagcaa aagacattat ggatgctggc aaactggtca ccgacgaact ggtgatcgcg
    45301 ctggttaaag agcgcattgc tcaggaagac tgccgtaatg gtttcctgtt ggacggcttc
    45361 ccgcgtacca ttccgcaggc agacgcgatg aaagaagcgg gcatcaatgt tgattacgtt
    45421 ctggaattcg acgtaccgga cgaactgatc gttgaccgta tcgtcggtcg ccgcgttcac
    45481 gcgccgtctg gtcgtgttta tcacgttaaa ttcaatccgc cgaaagtaga aggtaaagac
    45541 gacgttaccg gtgaagaact gactacccgt aaagacgatc aggaagaaac cgtacgtaaa
    45601 cgtctggttg aataccatca gatgacagca ccgctgatcg gctactactc caaagaagcg
    45661 gaagcgggta acaccaaata cgcgaaagtt gacggcacca agccggttgc tgaagttcga
    45721 gctgatctgg aaaaaatcct cggctaatca agccgcagca gatctgcttt cgggcaggtc
    45781 tgctcatcca cctcaggcaa tacctctcac agcaattagt tcttcttcct cacttttccg
    45841 ctacaattat caacaagttg aatcgataag aggcggtaat gcgtcagact aaaaccggta
    45901 tcctgctggc aaacctgggt acgcccgatg cccccacacc tgaagcggta aaacgctatc
    45961 tgaaacaatt tttaagcgac agacgcgtgg ttgatacctc acggttgtta tggtggccat
    46021 tgctgcgcgg cgtgattttg ccgctgcgct cgccgcgtgt ggcgaagctg tatgcctctg
    46081 tctggatgga aggtggctcg ccgctgatgg tttacagccg ccagcaacag caggcgctgg
    46141 cacaacgttt accggagacg cccgtagcgc tgggaatgag ctacggctcg ccatcactgg
    46201 aaagcgccgt agatgaactc ctggcagagc atgtagatca tattgtggtg ctgccgcttt
    46261 atccgcaatt ctcctgttct acggtcggtg cggtatggga tgaactggca cgcattctgg
    46321 cgcgcaaacg tagcattccg gggatatcgt ttatacgtga ttacgccgat aaccacgatt
    46381 acattaatgc actggcgaac agcgtacgcg cttcttttgc caaacatggc gaaccggatc
    46441 tgctgctgct ctcttatcat ggcattcccc agcgttatgc agatgaaggc gatgattacc
    46501 cgcaacgttg ccgcacaacg actcgtgaac tggcttccgc attagggatg gcaccggaaa
    46561 aagtgatgat gacctttcag tcgcgctttg gtcgggaacc ctggctgatg ccttataccg
    46621 acgaaacgct gaaaatgctc ggagaaaaag gcgtaggtca tattcaggtg atgtgcccgg
    46681 gctttgctgc ggattgtctg gagacgctgg aagagattgc cgagcaaaac cgtgaggtct
    46741 tcctcggtgc cggcgggaaa aaatatgaat atattccggc gcttaatgcc acaccggaac
    46801 atatcgaaat gatggctaat cttgttgccg cgtatcgcta aagctgagcg gtaaagaact
    46861 gagcgccgtc gcgaagagcc tcgtcggcgg ttttcatcat ccgtgaataa tgcaaaaagg
    46921 cgtgcagcgt gcctgggtag agtttgaact cacagggctg ctgatgcgcc gctaacgtct
    46981 ggtaaagcag acggctgtca tccagcagcg gatcgaactc cgccccggca ataaaacagg
    47041 gcggaacttc gcgagtgaga tcattattaa acagacagta atacggcgac tcgcggtccg
    47101 cgtcgttgct taaatacgcc tcttcataca tctgcaaatc ctgttgcgtt aagccatccc
    47161 agacaccgcc caacagacga cgagtcacgg aatcccgtaa tccgtaaagc ccataccaca
    47221 gcaaaacgcc cgcaacttta ccgcaatcga tctgtttatc acgcaaccac aacgcactgg
    47281 cgagcgccag catggcacct gcggaatcac cggcaaagcc aatgcgggac atattgattt
    47341 gataatcctc cgcctgctgg tggaaataac aacaagcagc cacaatttcc tctatcactt
    47401 gcggaaaacg cgcttcaggt gaaagggtgt aatcaatacc aatcaccgta cattggctgt
    47461 agcttgccag caggcgcatg atgcgatcgt gggtatcgag attgccgaga ataaaaccgc
    47521 ctccatgcaa ataaaatagc gttgccgggc tatctggctg cggacaaaag agacgtgttt
    47581 ccacctgccc atattttgtt ggaaccatgt aagctttggt tgccatttct ggagcgcccg
    47641 cattccagaa tcggcgctca agcgtgtaat actgtcgttg ctcagcaatc gttcccgttg
    47701 cgggccaggg cggtaaatcc ggctgaagag tattcacaac ggtcttcatt tcagcagaaa
    47761 taaggtccag aacaggtaat ttgttttccg gcttcataca aaaactcctt tcaaattacg
    47821 tcattgtaag gaaccactgc catgaaaatg cgatcccgcc tgctgatatt gaaactggct
    47881 gcgtctcgcg cgctcccgtc agattgtgtt aacattcgcc gctcagttaa ccacccgtaa
    47941 aaacaaccat gaaatttccc ggtaaacgta aatccaaaca ttacttcccc gtaaatgcac
    48001 gcgatccgct gcttcagcaa ttccagccag aaaacgaaac cagcgccgcc tgggtagtgg
    48061 gtatcgatca aacgctggtc gatattgaag cgaaagtgga tgatgaattc attgagcgtt
    48121 atggattaag cgccgggcat tcactggtga ttgaggatga tgtagccgaa gcgctttatc
    48181 aggaactaaa acagaaaaac ctgattaccc atcagtttgc gggtggcacc attggtaaca
    48241 ccatgcataa ctactcggtg ctcgcggacg accgttcggt gctgctgggc gtcatgtgca
    48301 gcaatattga aattggcagc tatgcctatc gttacctgtg taacacctcc agccgtaccg
    48361 atcttaacta tctacaaggc gtggatggcc cgattggtcg ttgctttacg ctgattggcg
    48421 agtccgggga acgtaccttt gctatcagcc ctggccacat gaaccagctg cgggctgaaa
    48481 gtattccgga agatgtgatt gccggagcct cggctctggt tctcacctct tatctggtgc
    48541 gttgcaagcc gggtgaaccc atgccggaag caaccatgaa agccattgag tacgcgaaga
    48601 aatataacgt accggtggtg ctgacgctgg gaactaagtt tgtcattgcc gagaatccac
    48661 agtggtggca gcaattcctc aaagaccacg tctctatcct tgcgatgaac gaagatgaag
    48721 ccgaagcgtt gaccggagaa agcgatccgt tgttggcatc tgacaaggcg ctggactggg
    48781 tagatctggt gctgtgcacc gccgggccaa tcggcttgta tatggcgggc tttaccgaag
    48841 acgaagcgaa acgtaaaacc cagcatccgt tgctgccggg cgctatagcc gaattcaacc
    48901 agtatgagtt tagccgcgcc atgcgccaca aggattgtca gaatccgctg cgtgtctatt
    48961 cgcacattgc gccgtacatg ggcgggccgg aaaaaatcat gaacaccaac ggagcagggg
    49021 atggcgcact ggcagcgttg ctgcatgaca ttaccgccaa cagctaccat cgtagcaacg
    49081 taccaaactc cagcaaacat aaattcacct ggttaactta ttcatcgtta gcgcaggtgt
    49141 gtaaatatgc taaccgtgtg agctatcagg tactgaacca gcattcacct cgtttaacgc
    49201 gcggcttgcc ggagcgtgaa gacagcctgg aagagtctta ctgggatcgt taagttatcg
    49261 tcggttcgta ggccagataa ggcgttcacg ccgcatctgg cattttgctc tcaatgcctg
    49321 atgcgacgct ggcgcgtctt atcatgccta catatttttc atattttaca tccggcaacc
    49381 accgtttacc ccgtcaccac ttcacccgcc ggtggcgttt ccagcagttc cagcatagta
    49441 cgggcaattt cacgctcgcc catcactact tgattcgcac cacgttcggt gatatacgcc
    49501 acttcatcgt cataatgggc gcgggcaata atctcaatat ccggattttt cgcgcgggca
    49561 gatgccacaa tctcacccgc ttcataaccg ttggggatcg tcaggatcag ccattttgca
    49621 cattccagat gcgccagttg cataatttct tcgttcgccg cattgcccaa tactgcgcgg
    49681 accccgcgct ctcgcagctc atcaacacgg gttcgtgacg tctcaatcac caccagcgga
    49741 atatcagagg cgagcaattt ctcccccagc aggctgccta cacgaccgta acccaccaat
    49801 agcgcatggt tgcaaatatc cactgggatc tgcttctctt cttcgattgc ctcttccagc
    49861 gtctgctctt ccagcgtttc ggtcttcgcc agatatttct ccagtagtgc gaacagtact
    49921 gggttgagca taatcgacag gatcgcccct gccagtacca ggttttgtcc ggcctgcggc
    49981 agtaaattca atgccattcc cagtcccgcc aggataaacg cgaactcacc aatctgcgcc
    50041 aggctggcgg cgatagttaa tgccgtacgt tgggagtgac caaacagtcg caccaggaaa
    50101 aacgcggcta acgacttacc aaacagaata atcgccagcg tcgccagcac tgccagcggt
    50161 tgctgaatca gaattaacgg atcaaacaac atcccgacgg agacaaaaaa cagcaccgca
    50221 aacgcgtcgc gcaatggcag cgtatcgtgg gcggcacggt gactcagttc agactcgttc
    50281 agtaccatcc cggcaaagaa cgcaccgagt gcaaaggaga catcaaacag ctctaccgca
    50341 ccaaaggcaa tccctaacgc cagcgccagc accgacaggg taaacagctc gcgagaaccg
    50401 gttgccgcgc tgcgtgccat aatccacggc accagacggc gacctaccag catcataatg
    50461 gcgataaacg cgatcacttt gccgatggtg atccccatat cgactgcaag agtggcaaag
    50521 cccacatcgc cctgttccat cattcctgcc actgcgggca gcaacaccag cgtcagaacc
    50581 attaccaggt cttccacaat caaccaaccg atggcgattt gcccacgctg actgtcaatt
    50641 aattgccgtt cttcaagtgc gcgcagtaac accacggtac tggcggtgga aagacataaa
    50701 ccgaacacga taccggtcat taacgaccag cccagcacgg cagagagcgc catacccagc
    50761 agcgtcgcca cggctatctg ggcgatcgca ccgggaatgg cgatggcctt taccgccatc
    50821 aaatccttga gcgaaaagtg cagacccacg ccaaacatca acagaatgac gcccagttca
    50881 gccagttccg gcgcaagctt ggtatcggca acaaagcccg gagtgaatgg tcctgccagc
    50941 acacccgcta acagatatcc caccagagga gaaatacgta gtttattggc cagcatgccg
    51001 aggataaagg cgagcacaag gccgccaaca atggtggtga taagcggggt ggcgtgatgc
    51061 attccgtctc cttttcctgg tggttattgt ccatttttgg ccgggaaaac caaaattaca
    51121 ggtaatagtt tatgacaatt tcattgatga tgttcatgaa taattattga attttgcaga
    51181 aaaatggaat tagctgcaaa aaagcacgga tcggaaaaca gaaggcggtt taacagagga
    51241 aaggcttatg gcgactgtaa aacgatgcag ccaaagtttg cctttggctg caatgaaatc
    51301 agtctttatg ccggttatca ggcaggaata tggtcaacat ccccaatagt ggcaggaaag
    51361 cacatatttt atagactaac tcgatgctgg tgtgatcggc gataagcccc agaactgccg
    51421 ctcccagacc tcccatgccg aaagcaaaac cgaaaaagag tccagaaacc ataccgatac
    51481 gtcctggaag cagctcctga gcgtagacca gaatggcaga gaatgccgaa gcgaggataa
    51541 atccaataat caccgttaaa acccccgtcc agtgcaggct ggcgtagggt aaaatcagcg
    51601 taaacggcgc aacgccgagg atagagcccc aaatcacata tttccgccca attttatccc
    51661 ctacaggccc gccgatcacc gtacctgccg caacggcaaa caggaaggca aacagatgaa
    51721 gctgagcatt ctggatagat aatccgaatt tttgcatcag ataaaaggtg taatagctgc
    51781 tgatgctcgc catatagaaa tatttcgaga aaatgaggat taacagaatg ctgaccgcca
    51841 gtaccacttt gttgcgcggc agtggattga taatcgtcgc tttgggtttt cctttattca
    51901 ttcggtgctg tgccgagtac caacggctga tttgcgccaa caccacgatc gccagcagtg
    51961 ccgcaagcac aaaccaggca acgttgcctt tgccataagg cgcgataatc accgccgcca
    52021 gcaagggtcc cagggaactg ccaaagttgc cgccgacctg aaagatagat tgtgccaggc
    52081 catgccgccc gccggaagcc atacgggcca cgcgagaaga ttccggatga aagaccgatg
    52141 aaccggtacc gaccagcgcc gccgccagca gaactgcgcc aaaactgccc gccagcgcaa
    52201 gcagcaccag accacttaag gtaaagcaca tgccaattgg caacgaccac ggcatcggat
    52261 atttatcggt ccagtagccg accactggtt gcagtagcga agaggcgagc tggaaggtga
    52321 gggttatcat gccaatctgc ataaatgtca gagaaaattc tgactgaagc agaggataaa
    52381 tcgccagaat cagcgattgg atcatgtcgt tcagcagatg tgagaggctg atagcaccta
    52441 aaataccaaa cgatgttcgg gccttggtcg ttgacgcagc cgcgcccgcc acaggctggg
    52501 tttgttcact cattgccata ggaaagtcac tttttcaggg ttgcgatgtt aagaatgatc
    52561 ttatttgtga ttattacccg actaacatac ctgtatgcgc cgcctgaagg aagtctcaac
    52621 gctgaataca gaatttctaa tctggatgca gatttatctt caccggacgc agacttgtct
    52681 atgatgtcgc gtcatactat ttttcaacac gttgaaatca ggtcagggag agaagtatga
    52741 aattattgca gcggggcgtg gcgttagcac tgttaaccac atttacactg gcgagtgaag
    52801 ctgctctggc gtatgagcag gataaaacct acaaaattac agttctgcat accaatgatc
    52861 atcatgggca tttttggcgc aatgaatatg gcgaatatgg tctggcggcg caaaaaacgc
    52921 tggtggatgg tatccgcaaa gaggttgcgg ctgaaggcgg tagcgtgctg ctactttccg
    52981 gtggcgacat taacactggc gtgcccgagt ctgacttaca ggatgccgaa cctgattttc
    53041 gcggtatgaa tctggtgggc tatgacgcga tggcgatcgg taatcatgaa tttgataacc
    53101 cgctcaccgt attacgccag caggaaaagt gggcaaagtt cccgttactt tctgccaata
    53161 tctaccagaa aagtaccggc gagcgcctgt ttaaaccatg ggcgctgttt aagcgtcagg
    53221 atctgaaaat tgccgttatt ggtctgacga cggatgacac agcgaagtta ggcaatccag
    53281 aaaacttcac cgatattgag ttccgtaagc ccgccgatga agcgaagctg gtgattcagg
    53341 agctgcaaca gacagaaaag ccagacatta ttatcgcggc gacacatatg gggcattacg
    53401 ataacggcga tcacggttct aacgcaccgg gcgatgtgga gatggcacgc gcgttgcctg
    53461 ccggatcgct ggcgatgatt gtcggcggtc actcgcaaga tccagtctgt atggcagcgg
    53521 aaaataaaaa gcaggtagat tacgttccag gtacgccatg caaaccggat caacaaaacg
    53581 gcatctggat tgtacaggcg catgagtggg gcaagtacgt tggtcgggcc gactttgagt
    53641 ttcgcaacgg cgaaatgaaa atggttaact accagctgat tccggtgaac ctgaagaaga
    53701 aagtgacctg ggaagacggg aaaagcgagc gcgtgctgta cacccctgaa atcgctgaaa
    53761 accagcaaat gatctcgctg ttatcgccgt tccagaacaa aggcaaagcg cagctggaag
    53821 tgaaaatagg cgaaaccaat ggtcgtctgg aaggcgatcg tgacaaagtg cgttttgtac
    53881 agaccaatat ggggcggttg attctggcag cacaaatgga tcgcactggt gccgactttg
    53941 cggtgatgag cggaggcgga attcgtgatt ctatcgaagc aggcgatatc agctataaaa
    54001 acgtgctgaa agtgcagcca ttcggcaatg tggtggtgta tgccgacatg accggtaaag
    54061 aggtgattga ttacctgacc gccgtcgcgc agatgaagcc agattcaggt gcctacccgc
    54121 aatttgccaa cgttagcttt gtggcgaaag acggcaaact gaacgacctt aaaatcaaag
    54181 gcgaaccggt cgatccagcg aaaacttacc gtatggcgac attaaacttc aatgccaccg
    54241 gcggtgatgg atatccgcgc cttgataaca aaccgggcta tgtgaatacc ggctttattg
    54301 atgccgaagt gctgaaagcg tatatccaga aaagctcgcc gctggatgtg agtgtttatg
    54361 aaccgaaagg tgaggtgagc tggcagtaat ccgaaagtgc cggatgttcg catccggcac
    54421 aatgcttaat cgcggcgggc gatatcagca aatttggcat cgaggatctt cgccagatcg
    54481 cctgccgcca gttcgatatc cagtccgcgc ttgccgccgg aaacataaat agtggcaaat
    54541 tcttgtgcgg gggcgtcgat aatcgttggc agacgttttt tctgccccag tgggctaatc
    54601 cccccaacca ggtatcccgt cgaacgctgc gcgaccatcg gatcggccat ctcaactttc
    54661 ttggcaccca gcgcttttgc tactttttta aggtccagtt gacctgcgac cggcgtaacg
    54721 gccacggcaa ggtgtttcat atcaccgttc actgcgacca gcagcgtttt gtagacctga
    54781 tccggattca aacctaattt tttgacgact tcatcgccaa aattggtttc agccggatcg
    54841 tgctcgtagg tatggatttg aaacgaaatc ttgttttttt cgagtgattt aactgcgggt
    54901 gtcatggtaa ttttcctgcc ttaaacaaaa aaacgaagca agcatacgcc tgatggcggt
    54961 ccaaaaaaaa gagtcatctt gcctaagagt attggcagga tggtgagatt gagcgacaat
    55021 cgagttacac cgtcgcttaa agtgacggca taataataaa aaaatgaaat tcctctttga
    55081 cgggccaata gcgatattgg ccattttttt agcgcaacat ttgcggtaaa ttcccttctc
    55141 catacaggtg tagtgcaccg accgcgacca catatcgccc cggcggcatg gcgcgtaatt
    55201 tatcccgcca ggcgagattt cgctgatgca tcagcacatc gtacagcgac tgactgaacg
    55261 tattgggcag cgttatttca ttattttgcg gcggcgcatt cagccaccag ctcatcattt
    55321 gttgcagcaa ccgtgcgttg gtatgccagt gggtcagcgt atcgtccagc agcgccagtc
    55381 ctttgtcagg gagctggagc aacatggcaa tctggttttc cgccccttcc agttcaatca
    55441 cgggtttatg ttgttgcttc gccgcctgca atagctgata atcgataccg tattctgccc
    55501 gcagccccag tttttgtgcc tgcgtcgcct gaagaaccat cgcgatttgc cacagcggtt
    55561 gggtagaaaa gagtgaggga gaaatgccca tctcctggct aatgtgctgc agtttttgga
    55621 gttgctcctc gctaatgcgc tcttccagcg cctcgcaggc aggcaaatta gcaaaaggcg
    55681 tatcgctggt ggaaacatcc gcctcgacga tcagcgcatc ggcgtttttg agctttttga
    55741 gcaaacgggt gggtagggga gccatatcgt ggctacccat atgaatactg ccaatcagat
    55801 gaaaatggcg attgccggga agagagatat caatggcagg ccaggtgtaa tgattaccgc
    55861 gcagcgcggc ccaaagcgtt tttacccggt acaacagatc catacgacct ccctttgtga
    55921 aatatcatgc tagcgcgcgg ggagggatgg cgcaaatgga gttggggact tgtttaatgt
    55981 gtttgtatga ttcagtatgt tcttgtatcg ctatttacag ggaagcaaca gttaaaaact
    56041 atgaaacagg caacaagaaa accgacgaca cccggagata ttcttctcta tgaatatctg
    56101 gaaccgctag atttgaaaat caatgagtta gcagagttgc tgcatgttca tcgtaatagc
    56161 gtcagtgcac tgatcaataa caatcgtaaa ctcaccactg agatggcatt ccgtctggcg
    56221 aaagtttttg ataccacagt cgatttttgg ctaaacctcc aggcggcagt tgatctttgg
    56281 gaagttgaaa acaacatgcg cacccaggaa gaattgggac ggattgaaac agtggctgaa
    56341 tatttggcac gccgtgaaga gcgtgcaaaa aaggtcgcgt aaggccaaaa catcgcttca
    56401 tctcgtaggc cggataaggc gttcacgccg catccggcat ccgagcacca ttgcctgatg
    56461 cgacgcttac gcgtcttatc aggcctacaa acctgtgcct gaaccgtagg ccggataagg
    56521 cgtttacgcc gcatccggca gtcatgcgtc gatgccaaat gcgccaccct aaagcagcgc
    56581 atccgcaatg acgtacttat tccttcggtt taaaccgcag caaccggttg gcgttactca
    56641 ctacggtaat cgacgagagc gccattgccg ctccggcaac taccgggtta agcagtgttc
    56701 cagtaaacgg ccacaaaata ccggcggcga ccggaatacc gatactgttg tagataaacg
    56761 cgccgagcag gttctgcttc atgttgcgca gcgttgcgcg ggaaatagcg agcgcatccg
    56821 caacgcccat caggctatgg cgcatcaggg taatcgccgc ggtttcaatg gcaacatcac
    56881 tgccgccgcc cattgcaatg ccgacatccg cctgggccag cgctggcgca tcgttaatgc
    56941 cgtcgcccac cattgccacc tgacgtcctt cactttgcag gcgtttaatc gcttcggctt
    57001 taccgtccgg cagcaccccg gcgatcacct catcaatccc tgcttctttg gcgatcgcat
    57061 tggcggtggt tgggttatcc ccggtcaaca tcaccagacg atatcccgct ttatgcagac
    57121 gttgcagcgc cgccacgcta tcactacgca acggatcgcg tacagccagc agggctaccg
    57181 ctttcccgtc aaccgccagc agcacaggcg ttgccccttg cgatgcctga gctgaaatat
    57241 ctgcttcaat cgctttagta tcaacttgct ggtcatttaa cagcgcctga ttgcccagca
    57301 ataacgcatg accttcagct tcaccgctca cgcccagccc gcgcaatgtg cggaaaccgt
    57361 tgacctgcgg cagctgcata tcgcttgctt tatcgaggat cgcccgcgcc agcggatggc
    57421 tggaaccttg ttccagtgcc gccgccagac gcagcgcctg cgcttcgtca aaatcagcaa
    57481 atgttttcac tgcgacaacc tgcggcttcc cttcggtcag cgtcccggtt ttatcgaaca
    57541 ctacggtatc gagtgtacta gcgcgttgca gcgcgtcggc gtcccgcacc agcacgccaa
    57601 actcagccgc ccgcccgacg ccggaaataa tcgacatcgg cgtcgccagc cccagcgcac
    57661 acggacaggc aataatcagc accgtggtgg caatcaccag ggtatagaca atctgcggtg
    57721 ccggaccaaa gaaataccag attgccgcac tgacaagcgc aataaccact actaccggca
    57781 caaatacggc tgagatttta tccgccagct gaccgatttc tggcttgctg ctctgggcct
    57841 ggcgcaccat acgaataatc cttgatagcg tggtatggct gccaaccgca ctggcgcgaa
    57901 acagaacgct accgtcctgt accactgtcc cggcatggac gctatcgcct tcgccttttt
    57961 gctgcgggat aggttcgccc gtcagcatcg cttcatccag ccatgcttcg ccctgggtaa
    58021 tctcgccatc tactggcacg cggtcgcccg tcgtcaggcg cagtagcatg cctggctgaa
    58081 cttccgccag cggcacgctt ttttcacctt cgtcagtaac caggcgcgcc gtcggcggag
    58141 ttaaatcgag taatttctcc agcgcctttg aagaacgctg acgtgcgcgc gcttccagca
    58201 tatgaccgag attaatcaga ccgataatca tcgcactggc ttcatagtaa agatgccgcg
    58261 cttccatcgg gaaccactgc ggccacaggt tgacgctcat cgaatagagc cacgccacac
    58321 ccgtacccag cgccaccagc gtatccatcg tcgccgcacc gttcagcagg cttttccatg
    58381 cactgcggta aaaatggccg ccggcgaaaa ccatcactgc cagggttatc aggccgataa
    58441 ccaaccacag gctgcggttg tcagcggtga ccatcatgtt atcgccgatc atcccccaga
    58501 ccattaccgg gatacccaca gccagtgcaa caattgcctg ccagcggaag cgcttcatcg
    58561 tagcgacggc ggtttcttgc tggcgctcgc ggcgtttcgc gtcatcttca atcgcttccg
    58621 cgccgtagcc cgctttttcc accgcctgca ctaaatcttg tggggaggca ctgcccatca
    58681 ccagcgcagt acgctccgcc aggtttaccc gtgcctgagt gacgcccggt acgctttgca
    58741 gcgcattttg tacgcgggtg acacagctgg cgcagctcat gccgctcagc agcaactgct
    58801 ggctgtcatc gtcatcggcg gtcgctgccg gaagcgcctc agaaaccgct gtcagtgctt
    58861 ccgacgggat tgatgactcc gccagcggtt tagcctttgg gtggcttaca gatgcgtcat
    58921 aacccgcttg tttgatggtt tcgatcagct gttctgcact ggcagtcccg gtaacgtgcg
    58981 cttcagtgat agacacatcc gcttgttcca catccgggcg ctgttcgaga ctttctttca
    59041 cgcgtttaac gcagtgaccg caggacaggc cgtccagggt caggtcgata gtttgtgaca
    59101 taaaacactc ctttaagaca gttttgactg gctgtgataa aggttaaacc ttccagcaag
    59161 gggaaggtca agaaattaat aacccaggcg agtaaaagtc cgtaaagatt aaaaaatcgg
    59221 ctcgatttgc atcaggatta gacatttatc tctttgtttt cctgtagtta agttgcgggt
    59281 gctaagttaa atcaggatgc ctgaaaatcg gcaccggggt gaggaattac ctcccgcatc
    59341 tataaaaagg agttaacaaa agatgttaga tgcaaacaaa ttacagcagg cagtggatca
    59401 ggcttacacc caatttcact cacttaacgg cggacaaaat gccgattaca ttccctttct
    59461 ggcgaatgtg cctggtcaac tggcagcagt ggctatcgtg accagcgatg gcaacgtcta
    59521 tagtgcgggt gacagtgatt accgctttgc gctggaatcc atctcgaaag tctgtacgtt
    59581 agcccttgcg ttagaagatg tcggcccgca ggcggtacag gacaaaattg gcgctgaccc
    59641 gaccggattg ccctttaact cagttatcgc cttagagttg catggcggca aaccgctgtc
    59701 gccactggta aatgctggcg ctattgccac caccagcctg attaacgctg aaaatgccga
    59761 acaacgctgg cagcgaattt tacatatcca acagcaactg gctggtgagc tggtagcgct
    59821 ctctgacgaa gtcaaccagt cggaacaaac aaccaacttc cataaccggg ccattgcctg
    59881 gctgctgtac tccgccggat atctctattg tgatgcaatg gaagcctgtg acgtgtacac
    59941 ccgtcagtgc tccacgctca tcaatactgt tgaactggca acgcttggcg cgacgctggc
    60001 ggcgggtggt gtgaatccgt tgacgcataa acgcgttctt caggccgaca acgtgccgta
    60061 cattctggcc gaaatgatga tggaagggct gtatggtcgc tccggtgact gggcgtatcg
    60121 tgttggttta ccgggcaaaa gcggtgtagg tggcggtatt ctggcggtcg tccctggagt
    60181 gatgggaatt gccgcgttct caccaccgct ggacgaagaa ggcaacagtg ttcgcggtca
    60241 aaaaatggtg gcatcggtcg ctaagcaact cggctataac gtgtttaagg gctgatcatg
    60301 atgaacacgg aaggtaataa cggtaacaaa cccctcggtc tatggaacgt cgtttccatc
    60361 ggcattgggg caatggtggg ggcggggatc ttcgcgctgc tggggcaggc tgcgttgcta
    60421 atggaagcct cgacctgggt cgcctttgct tttggcggta ttgtggcgat gttttccggt
    60481 tatgcctatg cgcgtctggg ggcgagctat cccagcaatg gcggcattat cgacttcttt
    60541 cgtcgcggat taggcaacgg cgtcttttcg ctggcgctct cgttactgta cctgttgacg
    60601 ctggcggtga gcatcgccat ggtcgcccgt gcttttggcg cttatgccgt gcagtttttg
    60661 catgaaggca gccaggagga gcaccttatt ttgctctacg cgttggggat cattgcggtg
    60721 atgacgcttt tcaactcctt aagcaaccat gcggtagggc ggctggaggt gatcctcgtc
    60781 ggcattaaaa tgatgatcct gttattgctg attattgccg gtgtctggtc gctgcaaccg
    60841 gcgcatattt ccgtctctgc gccccccagc tccggtgcgt tcttctcctg tatcgggata
    60901 accttccttg cctatgcggg ctttggcatg atggcgaacg cggcggataa agtgaaagat
    60961 ccgcaggtca ttatgccacg ggcgtttctg gtggcaattg gcgttaccac gttgctttat
    61021 atctcgctgg cgctggtttt gcttagcgat gtatcggcat tagagttaga aaaatatgcc
    61081 gataccgccg tagcgcaggc tgcttctccg ctgctcgggc atgtgggtta tgtgatcgtc
    61141 gtcatcggcg ctttactggc gacggcttca gccatcaacg cgaacctgtt cgccgtgttt
    61201 aacatcatgg acaacatggg cagcgaacgc gaactgccga agctaatgaa taaatccctg
    61261 tggcggcaga gtacctgggg caacatcatt gtcgtggtgt tgattatgct gatgacggcg
    61321 gcactaaatt taggctcact cgccagcgtt gccagcgcca cctttttgat ttgctatctg
    61381 gcggtgtttg tggtggcgat ccgcctgcgt catgatattc acgcctcgtt gccgattctt
    61441 atcgtaggta cgttggtgat gttgttggtg atcgttggct ttatctacag cctgtggtcc
    61501 cagggcagcc gtgcgttgat atggattatt ggtgcaatct tactcagcct tattgtggca
    61561 atggtcatga agcgcaataa aaccgtataa cacctctctg cgcgcagtac tgcctgtact
    61621 gcgcctttgt ctttaattc
//
LOCUS       DAZKIP010000015        59853 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZKIP010000015 DAZKIP010000000
VERSION     DAZKIP010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 59853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.015"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(191..838)
                     /gene="ychE"
                     /locus_tag="ACHOUK_001150"
     CDS             complement(191..838)
                     /gene="ychE"
                     /locus_tag="ACHOUK_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFP9896638.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(869..922)
                     /gene="ychE"
                     /locus_tag="ACHOUK_001151"
     CDS             complement(869..922)
                     /gene="ychE"
                     /locus_tag="ACHOUK_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFP9896639.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            1315..3990
                     /gene="adhE"
                     /locus_tag="ACHOUK_001152"
                     /gene_synonym="adhC"
     CDS             1315..3990
                     /gene="adhE"
                     /locus_tag="ACHOUK_001152"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFP9896640.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            complement(4291..4908)
                     /gene="tdk"
                     /locus_tag="ACHOUK_001153"
     CDS             complement(4291..4908)
                     /gene="tdk"
                     /locus_tag="ACHOUK_001153"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFP9896641.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            5512..5925
                     /gene="hns"
                     /locus_tag="ACHOUK_001154"
     CDS             5512..5925
                     /gene="hns"
                     /locus_tag="ACHOUK_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFP9896642.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(6069..6977)
                     /gene="galU"
                     /locus_tag="ACHOUK_001155"
     CDS             complement(6069..6977)
                     /gene="galU"
                     /locus_tag="ACHOUK_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFP9896643.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(7179..8192)
                     /gene="rssB"
                     /locus_tag="ACHOUK_001156"
     CDS             complement(7179..8192)
                     /gene="rssB"
                     /locus_tag="ACHOUK_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFP9896644.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(8284..9189)
                     /gene="rssA"
                     /locus_tag="ACHOUK_001157"
     CDS             complement(8284..9189)
                     /gene="rssA"
                     /locus_tag="ACHOUK_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFP9896645.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            9302..9760
                     /gene="ychJ"
                     /locus_tag="ACHOUK_001158"
     CDS             9302..9760
                     /gene="ychJ"
                     /locus_tag="ACHOUK_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFP9896646.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELIAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            9810..10652
                     /gene="purU"
                     /locus_tag="ACHOUK_001159"
     CDS             9810..10652
                     /gene="purU"
                     /locus_tag="ACHOUK_001159"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFP9896647.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVSRFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            10812..10896
                     /locus_tag="ACHOUK_001160"
     tRNA            10812..10896
                     /locus_tag="ACHOUK_001160"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10846..10848,aa:Tyr,seq:gta)
     gene            10941..11071
                     /locus_tag="ACHOUK_001161"
     ncRNA           10941..11071
                     /ncRNA_class="other"
                     /locus_tag="ACHOUK_001161"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            11106..11190
                     /locus_tag="ACHOUK_001162"
     tRNA            11106..11190
                     /locus_tag="ACHOUK_001162"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11140..11142,aa:Tyr,seq:gta)
     gene            11237..11368
                     /locus_tag="ACHOUK_001163"
     ncRNA           11237..11368
                     /ncRNA_class="other"
                     /locus_tag="ACHOUK_001163"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            11415..11546
                     /locus_tag="ACHOUK_001164"
     ncRNA           11415..11546
                     /ncRNA_class="other"
                     /locus_tag="ACHOUK_001164"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(11730..12407)
                     /gene="narI"
                     /locus_tag="ACHOUK_001165"
     CDS             complement(11730..12407)
                     /gene="narI"
                     /locus_tag="ACHOUK_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFP9896648.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(12407..13117)
                     /gene="narJ"
                     /locus_tag="ACHOUK_001166"
     CDS             complement(12407..13117)
                     /gene="narJ"
                     /locus_tag="ACHOUK_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFP9896649.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLPKEDAHALG
                     IFLRDLTTMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(13114..14652)
                     /gene="narH"
                     /locus_tag="ACHOUK_001167"
     CDS             complement(13114..14652)
                     /gene="narH"
                     /locus_tag="ACHOUK_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFP9896650.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(14649..18392)
                     /gene="narG"
                     /locus_tag="ACHOUK_001168"
     CDS             complement(14649..18392)
                     /gene="narG"
                     /locus_tag="ACHOUK_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFP9896651.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(18785..20176)
                     /gene="narK"
                     /locus_tag="ACHOUK_001169"
     CDS             complement(18785..20176)
                     /gene="narK"
                     /locus_tag="ACHOUK_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFP9896652.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            20515..22311
                     /gene="narX"
                     /locus_tag="ACHOUK_001170"
     CDS             20515..22311
                     /gene="narX"
                     /locus_tag="ACHOUK_001170"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFP9896653.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            22304..22954
                     /gene="narL"
                     /locus_tag="ACHOUK_001171"
     CDS             22304..22954
                     /gene="narL"
                     /locus_tag="ACHOUK_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFP9896654.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(22955..24349)
                     /gene="ychO"
                     /locus_tag="ACHOUK_001172"
                     /gene_synonym="iatA"
     CDS             complement(22955..24349)
                     /gene="ychO"
                     /locus_tag="ACHOUK_001172"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFP9896655.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            24535..24888
                     /gene="ychN"
                     /locus_tag="ACHOUK_001173"
     CDS             24535..24888
                     /gene="ychN"
                     /locus_tag="ACHOUK_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFP9896656.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(24932..25627)
                     /gene="chaC"
                     /locus_tag="ACHOUK_001174"
     CDS             complement(24932..25627)
                     /gene="chaC"
                     /locus_tag="ACHOUK_001174"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFP9896657.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     RPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(25788..26018)
                     /gene="chaB"
                     /locus_tag="ACHOUK_001175"
     CDS             complement(25788..26018)
                     /gene="chaB"
                     /locus_tag="ACHOUK_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFP9896658.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            26288..27388
                     /gene="chaA"
                     /locus_tag="ACHOUK_001176"
     CDS             26288..27388
                     /gene="chaA"
                     /locus_tag="ACHOUK_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFP9896659.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            27787..27894
                     /locus_tag="ACHOUK_001177"
     CDS             27787..27894
                     /locus_tag="ACHOUK_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP9896660.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            28323..28430
                     /locus_tag="ACHOUK_001178"
     CDS             28323..28430
                     /locus_tag="ACHOUK_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP9896661.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(28578..29432)
                     /gene="kdsA"
                     /locus_tag="ACHOUK_001179"
     CDS             complement(28578..29432)
                     /gene="kdsA"
                     /locus_tag="ACHOUK_001179"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFP9896662.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLRQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(29468..30277)
                     /gene="sirB1"
                     /locus_tag="ACHOUK_001180"
     CDS             complement(29468..30277)
                     /gene="sirB1"
                     /locus_tag="ACHOUK_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFP9896663.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(30281..30673)
                     /gene="sirB2"
                     /locus_tag="ACHOUK_001181"
     CDS             complement(30281..30673)
                     /gene="sirB2"
                     /locus_tag="ACHOUK_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFP9896664.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLTLVVLYIIIKLATTKVPLLG"
     gene            complement(30670..31503)
                     /gene="prmC"
                     /locus_tag="ACHOUK_001182"
     CDS             complement(30670..31503)
                     /gene="prmC"
                     /locus_tag="ACHOUK_001182"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFP9896665.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALARERPDCEITAVDRMPDAVALAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(31503..32585)
                     /gene="prfA"
                     /locus_tag="ACHOUK_001183"
     CDS             complement(31503..32585)
                     /gene="prfA"
                     /locus_tag="ACHOUK_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFP9896666.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(32627..33883)
                     /gene="hemA"
                     /locus_tag="ACHOUK_001184"
     CDS             complement(32627..33883)
                     /gene="hemA"
                     /locus_tag="ACHOUK_001184"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFP9896667.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEQVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            34097..34720
                     /gene="lolB"
                     /locus_tag="ACHOUK_001185"
     CDS             34097..34720
                     /gene="lolB"
                     /locus_tag="ACHOUK_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFP9896668.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            34720..35571
                     /gene="ispE"
                     /locus_tag="ACHOUK_001186"
     CDS             34720..35571
                     /gene="ispE"
                     /locus_tag="ACHOUK_001186"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFP9896669.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGANLS
                     PLHRAML"
     gene            35722..36669
                     /gene="prs"
                     /locus_tag="ACHOUK_001187"
     CDS             35722..36669
                     /gene="prs"
                     /locus_tag="ACHOUK_001187"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFP9896670.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            36794..38473
                     /gene="dauA"
                     /locus_tag="ACHOUK_001188"
     CDS             36794..38473
                     /gene="dauA"
                     /locus_tag="ACHOUK_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFP9896671.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(38528..38806)
                     /gene="ychH"
                     /locus_tag="ACHOUK_001189"
     CDS             complement(38528..38806)
                     /gene="ychH"
                     /locus_tag="ACHOUK_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFP9896672.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            39084..39668
                     /gene="pth"
                     /locus_tag="ACHOUK_001190"
     CDS             39084..39668
                     /gene="pth"
                     /locus_tag="ACHOUK_001190"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFP9896673.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            39785..40876
                     /gene="ychF"
                     /locus_tag="ACHOUK_001191"
     CDS             39785..40876
                     /gene="ychF"
                     /locus_tag="ACHOUK_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFP9896674.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            41720..44605
                     /gene="ycgV"
                     /locus_tag="ACHOUK_001192"
     CDS             41720..44605
                     /gene="ycgV"
                     /locus_tag="ACHOUK_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFP9896675.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAILFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSSIKTSGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPDPTPNPTPTPKPTTTADAGGNYLNVGYLLNYVENR
                     TLMQRMGDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMP
                     LYVGLYIGSTHASPDYSGGDGTARSDYMGMYASYMAHNGFYSDLVVKASRQKNSFHVL
                     DSQNNGVNANGTANGLSISLEAGQRFNLTPTSYGFYIEPQTQLTYSHQNEMAMKASNG
                     LNIHLNHYESLLGRASMILGYDITAGNSQLNIYVKTGAIREFSGDTEYQLNNSREKYS
                     FKGNGWNNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYHFSF"
     gene            complement(44705..46624)
                     /gene="dhaR"
                     /locus_tag="ACHOUK_001193"
     CDS             complement(44705..46624)
                     /gene="dhaR"
                     /locus_tag="ACHOUK_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFP9896676.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCVTPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            46852..47922
                     /gene="dhaK"
                     /locus_tag="ACHOUK_001194"
     CDS             46852..47922
                     /gene="dhaK"
                     /locus_tag="ACHOUK_001194"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFP9896677.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQVDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     GVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            47933..48565
                     /gene="dhaL"
                     /locus_tag="ACHOUK_001195"
     CDS             47933..48565
                     /gene="dhaL"
                     /locus_tag="ACHOUK_001195"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFP9896678.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSCVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPAALEAASS
                     IAEFAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            48576..49994
                     /gene="dhaM"
                     /locus_tag="ACHOUK_001196"
     CDS             48576..49994
                     /gene="dhaM"
                     /locus_tag="ACHOUK_001196"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFP9896679.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDNCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGAEIDKVIFDAMHALEAKREQLGLPSSDTEISDSCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSPL
                     TVEEEQERLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSPVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEEKLTLDVKTQRFNRQG"
     gene            50314..51998
                     /gene="treA"
                     /locus_tag="ACHOUK_001197"
                     /pseudo
     CDS             50314..51998
                     /gene="treA"
                     /locus_tag="ACHOUK_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
     gene            52334..53356
                     /locus_tag="ACHOUK_001198"
     CDS             52334..53356
                     /locus_tag="ACHOUK_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HFP9896680.1"
                     /translation="MSLTRRRFTQILASTLFLHHLPSFAQSVKFWASLTLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLSEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETWISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASRFVGEAQAFAASPAANLRFYAARGPRGLETGLKGSLHTEAAELLG
                     LHNVAQIADRQGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWHGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAT"
     gene            53356..54336
                     /locus_tag="ACHOUK_001199"
     CDS             53356..54336
                     /locus_tag="ACHOUK_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFP9896681.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVPV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACTA
                     ILWGLSIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFVLIVCATLITASTVAIAGIIGWVGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            54333..55091
                     /locus_tag="ACHOUK_001200"
     CDS             54333..55091
                     /locus_tag="ACHOUK_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9896682.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEVLLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLAARRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISH"
     gene            55101..55913
                     /locus_tag="ACHOUK_001201"
                     /pseudo
     CDS             55101..55913
                     /locus_tag="ACHOUK_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903984.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            55910..56764
                     /gene="modD"
                     /locus_tag="ACHOUK_001202"
     CDS             55910..56764
                     /gene="modD"
                     /locus_tag="ACHOUK_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HFP9896683.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQHGYIEFFLRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAIQNVLEW
                     SCGVSDYLDQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQDWSGAINQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPAASI"
     gene            56790..58760
                     /locus_tag="ACHOUK_001203"
     CDS             56790..58760
                     /locus_tag="ACHOUK_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFP9896684.1"
                     /translation="MRLKKHYLCTALSLAFTQQAVAAQESDSLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLQGPNQMGGAINITTQKPTKPLEASLGYRQG
                     WSRSQDNAYDMHASFAASSELGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMINSSA
                     DDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNSGQKSRYWQWPEYDKESFYY
                     QGTTQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQLAAD
                     VRENDLLTFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVAGISY
                     DWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFVNEDTLALSYYDRKRFPTLK
                     ERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTHDWGFEVSVYYNRVSDAILSH
                     NIDADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKITDLPT
                     QTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHGFSVN
                     ASVNNLFDTQYAYSEGFVEEGRNFWAGVEYTF"
     gene            complement(58810..59064)
                     /gene="ymgE"
                     /locus_tag="ACHOUK_001204"
     CDS             complement(58810..59064)
                     /gene="ymgE"
                     /locus_tag="ACHOUK_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP9896685.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            59265..>59853
                     /locus_tag="ACHOUK_001205"
     CDS             59265..>59853
                     /locus_tag="ACHOUK_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFP9896686.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWIGGQLISKIL
                     VITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AGLHEGMRFAQIEVNMGQWGVFHFDAQLISISERKV"
BASE COUNT        15058 a        15463 c        14725 g        14607 t
ORIGIN      
        1 gcccaggatt atgatgtggc gaaaataaca tgatgatgag gagaagcagt taaggaaatc
       61 cttataacta actgtaattt gagaagaata tttttaccta ttccttttgc ttttgataac
      121 tgcgcgggaa caaagtaaaa ataatgacga agtaaacgga gcatggcagc tccgtttcat
      181 ttgaaaggaa ttaattaagc aggccgggga aaatcccctt aataccagta acgataaatt
      241 caatccccaa tgccatcagc aatagcccca taatacgcgt aatcacgttg atgccggtct
      301 ggcgtaaaac ccgtaccagc cacggtgcca tgcggaacaa tccccaacaa cataaagcga
      361 acaatgcaat agccacaaag aaaccaaaca gatagctaat gctgtgataa cgcgtacccc
      421 agacgatggt agaactgatc gcccctggcc ccgccatcaa cggcaacgcc aatggcacca
      481 caccaatact ttcacgtacc gcggtttctg atttttcttg cttgttctgt ttatcctcgc
      541 caagcttgcc gctgatcatc gacatcgcta tcgtcaccac caggatgccc ccggcgatac
      601 ggaacgaatc aattgatata ccaaaaagtt gcagaatcgt gtcgccgaga aaaagcgaga
      661 tccacaagat aatggccaca gacaggttgg ctgtaaggtt agttttgttt cgcgctgccg
      721 ctgtctgata actggtcatg ctgataaaga cgggaataat ccctaccggg ttgaccagcg
      781 caaataaccc gatgaaaaat ttgaagtaaa cgggaaaatc aaaaagggtc tgaatcacgg
      841 ttagctccga agcaaaagcc ggataatgtt agccataaat aaggttgaaa agacgcgctg
      901 acaatacgcc ttttgacagc atttttcacc tcctaactac ttaaaattgc tatcattcgt
      961 tattgttatc tagttgtgca aaacatgcta atgtagccac caaatcatac tacaatttat
     1021 taactgttat ctataatggc gaaaagcgat gctgaaaggt gtcagctttg caaaaatttg
     1081 atttggatca cgtaatcagt acccagaagt gagtaatctt gcttacgcca cttggaagtg
     1141 acgcattaga gataataact ctaatgttta aactctttta gtaaatcaca gtgagtgtgt
     1201 gcgcgagtaa gcttttgatt ttcatcggtt aagcaaatca tcaccgcact gactatactc
     1261 tcgtattcga gcagatgatt tactaaaaaa gtttaacatt atcaggagag cattatggct
     1321 gttactaatg tcgctgaact taacgcactc gtagagcgtg taaaaaaagc ccagcgtgaa
     1381 tatgccagtt tcactcaaga gcaagtagac aaaatcttcc gcgccgccgc tctggctgct
     1441 gcagatgctc gaatcccact cgcgaaaatg gccgttgccg aatccggcat gggtatcgtc
     1501 gaagataaag tgatcaaaaa ccactttgct tctgaatata tctacaacgc ctataaagat
     1561 gaaaaaacct gtggtgttct gtctgaagac gacacttttg gtaccatcac tatcgctgaa
     1621 ccaatcggta ttatttgcgg tatcgttccg accactaacc cgacttcaac agctatcttc
     1681 aaatcgctga tcagtctgaa gacccgtaac gccattatct tctccccgca cccgcgtgca
     1741 aaagatgcga ccaacaaagc ggctgatatc gttctgcagg ctgctatcgc tgccggtgct
     1801 ccgaaagatc tgatcggctg gatcgatcaa ccttctgttg aactgtctaa cgcactgatg
     1861 caccacccag acatcaacct gatcctcgcg actggtggtc cgggcatggt taaagccgca
     1921 tacagctccg gtaaaccagc tatcggcgta ggcgcgggca acactccggt tgttatcgat
     1981 gaaactgctg atatcaaacg tgcagttgca tctgtactga tgtccaaaac cttcgacaac
     2041 ggcgtaatct gtgcttctga acagtctgtt gttgtcgttg actctgttta tgacgcagta
     2101 cgtgaacgtt tcgcaaccca cggcggctat ctgttgcagg gtaaagagct gaaagctgtt
     2161 caggacgtta tcctgaaaaa cggtgcgctg aacgcggcta tcgttggtca gccagcctat
     2221 aaaattgctg aactggcagg cttctctgta ccagaaaaca ccaagattct gatcggtgaa
     2281 gtgaccgttg ttgatgaaag cgaaccgttc gcacatgaaa aactgtcccc gactctggca
     2341 atgtaccgcg ctaaagattt cgaagacgcg gtagaaaaag cagagaaact ggttgctatg
     2401 ggtggtatcg gtcatacctc ttgcctgtac actgaccagg ataaccaacc ggctcgcgtt
     2461 tcttacttcg gtcagaaaat gaaaacggct cgtatcctga ttaacacccc ggcttctcag
     2521 ggtggtatcg gtgacctgta taacttcaaa ctcgcacctt ccctgactct gggttgtggt
     2581 tcctggggtg gtaactccat ctctgaaaac gttggtccga aacacctgat caacaagaaa
     2641 accgttgcta agcgagctga aaacatgttg tggcacaaac ttccgaaatc tatctacttc
     2701 cgccgtggct ccctgccaat cgcgctggat gaagtgatta ctgatggcca caaacgtgcg
     2761 ctcatcgtga ctgaccgctt cctgttcaac aatggttatg ctgatcagat cacttccgta
     2821 ctgaaagcag caggcgttga aactgaagtc ttcttcgaag tagaagcgga cccgaccctg
     2881 agcatcgttc gtaaaggtgc agaactggca aactccttca aaccagacgt gattatcgcg
     2941 ctgggtggtg gttccccgat ggacgccgcg aagatcatgt gggttatgta cgaacatccg
     3001 gaaactcact tcgaagagct ggcgctgcgc tttatggata tccgtaaacg tatctacaag
     3061 ttcccgaaaa tgggcgtgaa agcgaaaatg attgctgtca ccaccacttc tggtacaggt
     3121 tctgaagtca ctccgtttgc ggttgtaact gacgacgcta ctggtcagaa atacccgctg
     3181 gcggactatg cgctgacccc ggatatggcg attgtcgacg ccaacctggt tatggacatg
     3241 ccgaagtccc tgtgtgcttt cggtggtctg gacgcagtaa ctcacgccat ggaagcttat
     3301 gtttctgtac tggcatctga gttctctgat ggtcaggctc tgcaggcact gaaactgctg
     3361 aaagaatatc tgccagcgtc ctaccacgaa gggtctaaaa atccggtagc gcgtgaacgt
     3421 gttcacagtg cagcgactat cgcgggtatc gcgtttgcga acgccttcct gggtgtatgt
     3481 cactcaatgg cgcacaaact gggttcccag ttccatattc cgcacggtct ggcaaacgcc
     3541 ctgctgattt gtaacgttat tcgctacaat gcgaacgaca acccgaccaa gcagactgca
     3601 ttcagccagt atgaccgtcc gcaggctcgc cgtcgttatg ctgaaattgc cgaccacctg
     3661 ggtctgagcg caccgggcga ccgtactgct gctaagatcg agaaactgct ggcatggctg
     3721 gaaacgctga aggctgaact gggtattccg aaatctatcc gtgaagctgg cgttcaggaa
     3781 gcagacttcc tggcgaacgt ggataaactg tctgaagatg cattcgatga ccagtgcact
     3841 ggcgctaacc cgcgttaccc gctgatctcc gagctgaaac agattctgct ggatacctac
     3901 tacggtcgtg attatgtaga aggtgaaact gcagcgaaaa aagaagccgc tccggctaaa
     3961 gctgagaaaa aagcgaaaaa atccgcttaa tcagtagcgc tgtctggcaa cataaacggc
     4021 cccttctggg gccgtttttt tgtttaccca aagcaacttt tccataaacc gacagcatta
     4081 gccttcatca tatttgcgac gatgtataac gcctaaacac agggatattg tactttacag
     4141 gtcacaagtc aacgtcggtg cttaagagcc ctgtgaggcg tatagcggcg ttaaaaactg
     4201 ccgagaaggg tgtatagccc ggaagaagtg cgtaaaacga actgacagga taaaagtgcc
     4261 ctgctcaccc tgtcagtaaa gaaattctta ttaatcgtgg cgatgccttt cctgaatagc
     4321 cgttaatgag ccgacttgta acgcctcttt atagtgttta cggcatacag aaacgtatcg
     4381 ttcattacca ccaataacca cctgctcacc ttcgttataa ggtctgcctg cttgatcaag
     4441 acgcagcacc atgcttgctt tacggccaca aaaacagatg gtttttaatt caaccagttt
     4501 gtcggaccat gccagtaagt attggctgcc aataaataat tcacctcgaa aatcggtacg
     4561 taaaccataa caaagtacgg gtatatcgag ttgatcgaca acctccgata attcatatac
     4621 ttgttgtctg gttaaaaact ggcattcatc aaccagtacg caatgaattg cctgctgctc
     4681 atgttccgca cgaatctcat caaataatga tgaattttgg ttaaataatt ttgcaggcga
     4741 tgacaaacct atacgcgaac tgactttccc ggcaccaaag cgatcatcaa tttctgccgt
     4801 atatacgaca gtgcgcatgc cgcgttcctg gtaattgtat gaagattgca acaatgctgt
     4861 agacttaccc gcattcattg cggaatagta gaaatatagc tgtgccatca gccacaggcc
     4921 ctcaatgata tgaaataaag ttggctggag tttatcataa ttcgtcagtt tttcagctca
     4981 acagtatgca cagaagtggt atatgcgttc tcccttacga agccttgcat aatccttctg
     5041 agctatcatt acaactgcct cgcttgttat aagcgggtaa atgactgctg gtaactattc
     5101 acaatcttta acctgttgcg caagtaatag ccctctgttg acctccagga gatagtgcaa
     5161 tactaagtcc ctgctcttat tgcgactgtt ctacttttca tcattcgctt aatagggaat
     5221 tctcgtaaac acgactaata cagaagactg aaaggtcgtc agcctacgat aatctcccca
     5281 taaaatgtga catgaatcag gaagttttaa cctcacgtgc tgcgaaatca tcggtacaaa
     5341 tagggctata tgccgcgtct tttctggcta attttatgaa aagatattta ttggcggcac
     5401 aaaataaaga acaattttga attccttaca ttcctggcta ttgcacaact gaatttaagg
     5461 ctctattatt acctcaacaa accaccccaa tataagtttg agattactac aatgagcgaa
     5521 gcacttaaaa ttctgaacaa catccgtact cttcgtgcgc aggcaagaga atgtacactt
     5581 gaaacgctgg aagaaatgct ggaaaaatta gaagttgttg ttaacgaacg tcgcgaagaa
     5641 gaaagcgcgg ctgctgctga agttgaagag cgcactcgta aactgcagca atatcgcgaa
     5701 atgctgatcg ctgacggtat tgacccgaac gagctgctga atagccttgc cgccgttaaa
     5761 tctggcacca aagctaaacg tgctcagcgt ccggcaaaat atagctacgt tgacgaaaac
     5821 ggcgaaacta aaacctggac tggccagggc cgtactccag ctgtaatcaa aaaagcaatg
     5881 gatgagcaag gtaaatccct cgacgatttc ctgatcaagc aataatcttt tgtagattgc
     5941 acttgcttaa aatcccgcca tcggcgggat tttttattgt ccggtttaag acaatttaat
     6001 aagaataagc tataaaaaaa cggcgtcgat tgctcaacgc cgtttcgtgg ataacaccga
     6061 tacggatgtt acttcttaat gcccatctct tcttcaagcc aggctttaaa ttccgtgcca
     6121 agggtgttat gacgaatacc gtattcaacg aaggcctgca tgtaacctaa tttattaccg
     6181 cagtcatggc tcttcccttt catatgatag gcttcaaccg tttctttttc gattagcata
     6241 tcaattgcgt cggtgagctg aatttcatca ccagctcccg gaggggtttt tgccagcaac
     6301 ggccaaatat ccgcactaag tacgtaacga cccacaatag cgagattaga cggcgcaaca
     6361 tccgcttttg gtttttcaac cacgccaacc atcggtacgc tttcacccgg cgctaattca
     6421 acgcctttgc aatccacaac gccatatgcg gtcacatcag caaccggttc aaccatgatc
     6481 tggctatgac ccgtttcatc gaagcggcgg atcatctctg ccaggttatc ctgtgacaaa
     6541 tcggattcat attcatccag aataacgtca ggcaaaataa cagccacagg ttcatcaccc
     6601 actaccgggt gagcacacaa taccgcgtgt cccaggcctt tcgccagccc ctgacgaact
     6661 tgcataatag ttacgtgcgg cggacaaata gactgcactt catcaagcag ttgacgtttt
     6721 acacgttttt ccagcattgc ttccagttca aaactggtat caaagtggtt ttcaatagag
     6781 tttttagatg agtgtgtaac cagcacaatt tcagtaatgc cagccgcaat acattcattc
     6841 acgacgtatt gaattaatgg cttatcgaca agtggcagca tctctttcgg gatggctttc
     6901 gtcgccggca acatcctggt tcctaatccc gcaacgggga taacggcttt tttgactttc
     6961 gtattaatgg cagccattta aattctcctg gactgttcgt gttttgaacg tgttcatatt
     7021 tctgtatcgc atcccagtat atcagcaccc cggaggggct aagttctgaa aaggatgcga
     7081 gttagcataa ttaagacaat tacttatacg aatggcgacg atagtaccac cagccgacat
     7141 tagctggtaa tgcaaattta gcccgcgtta tcgtttgctc attctgcaga caacatcaag
     7201 cgcagtcgac caccggttcc ccatatttgg cattgccagg catcgcatcg ctggctcaat
     7261 tgattcaaat aagcgttacc taaagtgcct aacggaacac cattactgat ttgcacctgg
     7321 tgttcgccgg tatttaacgt cgcattcaga cccgcagaaa ccagaatgag atttttcagt
     7381 tcgcgatgat aatagccaac taatagcgga aactgccccg gcagattggc ctgacggagt
     7441 aaatggttta cctgcttcaa taacgcgccc aactctggca accgttgatt ttggtgtgca
     7501 agctgttcct gtaataatcc gttaaacaat gcgcgtaata acaaggcagc aagtacgcca
     7561 ttatgtccag ctcgggttac atcaaggcaa taaaatgcca gatcgttttc cgaaagtgcg
     7621 gcaatatcaa gcaccaggcc gggtttgtcc gcggcaacca attgacggta attaacccgg
     7681 caatgggaaa tcacctgctg aaccggcggt tgtagttcct gtaataattt cgccgctgcg
     7741 gcagggttat caaccattgc atcccagtcg cgaaaaagcc tttcctcttc ctcaacgcgc
     7801 gaattaaaca tgctgggata gagacaggca aaaaccatct cgcgcaagcg attcagatct
     7861 ttaactggtt tcagcaaaac atcttcaacg cccagacgta acgctttggc aatatctgcc
     7921 atattttcag tggcagatat caccagaact ggggtctggt cgcctctgtt acgtatatgc
     7981 tccagcagtt taagcccgtt cattcgtggc atcgcgatat cacatatcat caggtctgga
     8041 gtgaaacctc ccagcaactc aagggcatcc accccatcag ccgccagtac cgttgtcgct
     8101 cccaatgagg aaaaccacga atccagaagc gagcgaaata cctgctcatc ttcaacaatg
     8161 agaatctgtt ttccgaccaa tggctgcgtc atgttctctc ccctgactgg ctttactcaa
     8221 tagtggcatg ctattgccac ctgcgcctgt cagaattaac ttaagtgtag ataaaaattc
     8281 tggtcaaatg ttggtgcgta ccaacggcaa aagttcgtcc attttctttt ccactgccag
     8341 ctgcccggcc gcaatggcag cgtgcgcgcg atggaaatca agcgtagata tttgcgggca
     8401 aacaggttga atcagaatat cgggcggatc acctgccatg cggttccttt taaggcggtt
     8461 ctccagcacc tggatagaag tggtcataat ctctgttgcc gttggtgctg tcaccgcccg
     8521 acgtgtcgtt atactgccta acctttcttt cagacgcgca tgccacggca gagaatcacc
     8581 attctcgcta ttttcttcac tgacattaaa ggagagcaaa tcttgttgca tcaaatgagc
     8641 atcgtgctgc aggtcgaccg ctatcacaat atcagcaccc aatgcacgcg tgagcgaaat
     8701 aggaattggg ttaacgactg ccccatcaac cagccagtag ccgttatgcg caacgggtgc
     8761 cattagtcct ggaatactgc atgatgcgcg aatagcaaga tggagatcgc cttcagtaaa
     8821 ccataattca cgcccagtac ttaaattggt ggcaacagcc gcgaagcgac gggaacaatt
     8881 ttcgatctct gtttccggca ttatttcgcg gtattgattg aagacacgct cgccgcgcag
     8941 taacccaccg cgctgccagg agagatccat cagtcgtaaa acatcccaat aactgaaaga
     9001 ggtcacccaa tcttccagcg cagataatcg atcgcatgca taggccgcgc ccaccagcga
     9061 accaattgaa catcctgcaa cgatatcaat ttcaataccc acttttttta gcgcattaat
     9121 aacgccaata tgcgaccaac ctctcgccgc gccagatccc agcgccagcc ctatttttat
     9181 ctttctcatt attcccgcaa gattcccctg gaatgcaatc gtagccacat cgctactgct
     9241 cggttaacat agtgctactc aggcggttaa tacgccgtta ttgtttccag ggagattatt
     9301 tgtgtctcag ctttgtccct gtggtagtgc tgtcgagtat agcctatgtt gccaccctta
     9361 tgtgtctggt gaaaaggttg cacctgatcc agaacatctc atgcgttcgc gttactgcgc
     9421 ttttgtgatg caagacgcag attatttaat aaagacctgg catccatctt gtggagcagc
     9481 ggcgttacgt gccgaattga ttgccggatt tgcgcatact gaatggttgg gattgaccgt
     9541 atttgaacat tgttggcagg acgccgacaa tatcggattt gtcagttttg ttgctcgatt
     9601 taccgaaggt ggcaaaacag gtgcaataat tgaacgttcg cgatttttga aagaaaacgg
     9661 tcaatggtac tatattgacg gtacacgtcc gcagtttggc cgcaacgatc cctgcccttg
     9721 tggttcaggt aaaaaattta aaaagtgctg cggccaataa tggttgacgg tacggtttag
     9781 caaacactct caacaaggtt tttccagcaa tgcattcact ccaacgtaaa gttctgcgta
     9841 ctatttgtcc ggaccaaaaa ggtctgatcg cacgtattac caatatttgc tacaagcacg
     9901 agttaaatat cgtacagaac aatgaatttg ttgatcaccg taccgggcgc ttttttatgc
     9961 gcacggaact ggaagggatt tttaatgatt ccaccctgct ggcggatctc gatagcgcat
    10021 tgccagaagg ctccgtgcgt gagctgaatc ctgccggtcg tcgccggata gtgattctgg
    10081 tcactaaaga agcgcattgc cttggcgatt tgttgatgaa agccaattat ggcggcctgg
    10141 atgtcgaaat cgcggcagtg attggtaacc acgatacttt acgttctctg gttgagcgtt
    10201 ttgatatacc gtttgagctg gtaagccatg aagggttaag ccgcaacgag cacgatcaaa
    10261 agatggcgga tgccattgat gcttatcaac ctgactacgt ggtgctggcg aagtatatgc
    10321 gggtattaac accggaattt gtgtcacgct tcccgaataa gatcatcaat attcaccatt
    10381 ccttcctgcc agcgtttatc ggcgcacgtc cttatcacca ggcctatgaa cgtggcgtga
    10441 agattattgg cgcaaccgct cactatgtga atgacaatct ggacgaaggc ccaatcatca
    10501 tgcaggacgt tattcatgtc gatcatacct acacagctga agatatgatg cgcgcaggtc
    10561 gtgacgtcga gaaaaacgtc ttaagtcgcg cgctctacaa agtactggcg cagcgcgtct
    10621 ttgtttacgg taatcggacg attattcttt aatcgccagc aaaaataact ggttaccttt
    10681 aatccgttac ggatgaaaat tacgcaacca gttcattttt ctcaacgtaa cactttacag
    10741 cggcgcgtca tttgatatga tgcgccccgc ttcccgataa gggagcaggc cagtaaaaag
    10801 cattaccccg tggtggggtt cccgagcggc caaagggagc agactgtaaa tctgccgtca
    10861 tcgacttcga aggttcgaat ccttccccca ccaccatgat tcaccacagc gatgtggtaa
    10921 cttcggtgag attgtgaaag tttgcactca tcggggaagg gtgagaacct tcgactaagg
    10981 ttcgactcga gcgaaagcga gagaacgttt tcacaggcaa cggcccgaag ggtgaggagc
    11041 gaagcgacga ataatccttt ccccaccgcc atctcaagca ttacctcaaa atttgaatta
    11101 cccctggtgg ggttcccgag cggccaaagg gagcagactg taaatctgcc gtcatcgact
    11161 tcgaaggttc gaatccttcc cccaccacca tcactttcaa aagtccctga actctcaagc
    11221 gaatccgtaa ataaatattc tgcccaagcg gagaaggatg agaagcttcg actaaggttc
    11281 gactcgagcg ccagcgagag agcgttgccg caggcaacga cctgaagggc gaagcgcgca
    11341 gcgctgagta atccttcccc caccaccatc actttcaaaa gtccctgaac tctcaaacga
    11401 atccgtaatt aaatattctg cccaagcggg gaaggatgag aagcttcgac caaggttcga
    11461 cttgagcgcc agcgagagag cgttgccgca ggcaacgacc cgaagggcga agcgcgcagc
    11521 gctgagtaat ccttccccca ccaccatcgc ttcctgaaat aatcaaaacc tgttactccg
    11581 agctaatttt gcccaatggc accacgttta ccggacctac aatatcctcc cttgtaggtc
    11641 ggataagcga agcgcatccg acaactcacc ccgcaccgtg cccataaaaa aaccccgccg
    11701 aagcagggtc tatgtgaact aaaattcgct tagtgacgag cgcgcaccag ctggtactta
    11761 cgtgtcagat actccaccgg tacgctccag atgtgtacca gacgcgagaa cgggaacagc
    11821 aggaataacg tcatccccag caccaggtgt agacggaaga taaacgccac accatcgagg
    11881 tgttgagaag cgccaccgtg gaaggtcact accgactgcg cccagccaac cagtttcatc
    11941 atctcgctac cgtccatatg ttgggcggag aacggaatgg tcaacaggcc cagcgcgcac
    12001 tggataacga gcagcgacag gatcagtata tccgctccgg tagtcgttgc acgcacgcgt
    12061 gggctgaaca gacgacgttt aagcaaaagc acgccgccaa tcagacacag cacgccgctg
    12121 gcaccaccag caaacatcgc cattttctgt ttcacttcaa tcggcagcca ggcttcatac
    12181 atccagtgcg gcgtcaacat accgaagaag tgaccgacaa aaatccccag aataccgata
    12241 tggaacaggt tcgacgccag gttcatccct ttgcgatcca gcatctggct ggacgccgcg
    12301 cgccaggtgt actgcccgta gtcataacgc agccagctac caatcaggaa gaccgccccg
    12361 gctatgtacg ggtagatatc aaagaagaac atattcagga attgcattag tgctgtcctc
    12421 cggtggtgat attcagatat tgcggcgcga cggcaccggc aaagcgacgc tgatgagcag
    12481 tgattgctga atcaccgcag cctttgtcag caaagaattt aacctgctct tcttcccaaa
    12541 cagcatccag cgcctgcggc gtatcatcgc gcgcttcgtc ggcaattttt tccgccactt
    12601 tgtcgctgtc gatagcggta ttggccaatt tcagcagcag atcaaacagc acggcataac
    12661 ggctttcacg ctgttgcaga cgcgcactca gcaatgccag aatcggcgcg atatctttca
    12721 aaccttccac ggcttcgctt tgcggcagct gcgccaggta ctccagatac agcggcagat
    12781 gatccggcaa ttcgcggctg tttaactgca agccgtgctg ctcgtactgc gccagcaggt
    12841 ccaccatcgc ctggccgcgg tcgcgggatt cgccgtgcac atgttcaaac agcaacagtg
    12901 acgtggcgcg gccacggtcg aacagttcgc tgtactgcgc ctgggcatcg agcggatcca
    12961 tcgtcgttaa atcgcgcagg aaaatgccca gcgcatgggc atcctctttt ggcagatttt
    13021 tcgacgcggc aatcgcctca aacatctctt gttgatgctg ccataaggca gcatccggat
    13081 attcaaggag acgcgataca atcacgagtt cgatcatgga tgcggctccg ttttgctggt
    13141 cacatcgatg gcatcgatac gacggctgtt gaacagattg aatttggtat ctgaaccgtg
    13201 gcagccatca ccaaaggtaa agccgcagcc atttttctcc gggaaggctt cccgtgccag
    13261 ttcacgatga ctactcggca ccacaaagcg atcttcgtag ttagcaatcg ccagataacg
    13321 gtacatctcc tgtgcctggg cttcggtcag accgacctct tccagcgcac gggtatcaac
    13381 tttaccgtca acggtttcag cacgtttgta atgacgcatc gccagcatac gtttcagcgc
    13441 gcgcagtacc ggtttggtat caccggcggt cagcagattc gccagatact gtaccggaat
    13501 acgcaggctt tcgacgtctg gcagaatgcc gttgctaccc agctcacccg cgtctgctgc
    13561 agactgaatc ggagagagag gcggcacgta ccagaccatc ggcagtgtgc gatattctgg
    13621 atgcagcggc agcgccagtt tccattccat tgccatttta taaaccggcg actgctgtgc
    13681 agcttcaata acgctcagcg gaataccgtc tttaatcgcc tgctcgatga ctttcggatc
    13741 gttcggatcg aggaacacct ccagctgacg ctggtagaga tctttctcgt tctcggtgct
    13801 ggctgcacgt tcaatcgcgt cggcatcgta cagcagcacg ccaagataac ggatacgacc
    13861 gacacaggtt tctgagcaca cggtcggctg acccgcttca atacgcggat agcagaagat
    13921 gcacttctca gacttaccgc tcttccagtt gaagtagatt tttttgtacg ggcatccggt
    13981 gatgcacata cgccagccac ggcatttatc ctggtcgatc aggacgatgc catcttcttc
    14041 acgcttgtaa atcgcaccgc tcgggcaggt cgccacacat gccgggttca ggcagtgttc
    14101 gcacaggcgc ggcaaataca tcatgaaggt gttttcgaac tggctataca tcgccttctg
    14161 gatgttgtcg aagttcttgt ctttcgccag tttgtcaaac tcaccgccca ggtcatcttc
    14221 ccagttcggc cctttttcga ttttcgccat ccgttccccg gtaatcagcg aacgcggacg
    14281 ggcaatcggc tgcgatttgc tgccttccgg cgcggtatgc aggttctgat agtcaaaatc
    14341 gaacggctcg taataatcgt cgatccccgg cagatgcggg ttagcgaaga ttttacccag
    14401 cagcatggca cggttaccca tgcgcggttg cagtttgccg ttgattttac ggatccagcc
    14461 gcctttgtat ttttcctggt tttcccagtc agtcgggaag ccctggcccg gcttggtttc
    14521 cacgttgttg aaccacgcgt attccacgcc ttcacggctg gtccagacgt ttttacaggt
    14581 aactgaacag gtgtggcagc cgatgcactt atcgagattc agcaccatgc cgacttgtga
    14641 acgaattttc attttacgct ctcctgtacc tgatcattgc cttcgccatc taaccagtca
    14701 atgttcttca tcttacgcac tacaacaaac tcatcgcggt tagaacctac ggtgccgtag
    14761 tagttaaagc cgtatgccag atgggcatag ccgccgatca tatgcgtcgg tttcggcgtg
    14821 atacgggtga ccgagttatg gataccacca cgctgttggg taatttccga accaggcagg
    14881 ttaacgatac gttcctgcgc gtggtacatc atggtcatcc ctgccggaac acgctggctg
    14941 acaaccgcac gggcagtcag agcaccgttg ctgttgaata cttcaatcca gtcgttatcg
    15001 gcgataccca gatctttggc atcggcttca ctcaaccaga ccaccggacc accgcgacct
    15061 aaagtcagca tcagcaggtt gtcgctgtag gtggagtgga taccccactt ctggtgcggc
    15121 gtcaggaagt tgagcgcttt ttccgggttg ccgttggatt tctggcctat cacttctttc
    15181 accgaacggg tgtcgatcgg cggacgataa accagcaggc tttcaccgaa atcacgcatc
    15241 cactggtgat cctgatacag ttgctgacga ccagagagcg tacgccatgg gatcagctcg
    15301 tgaacgttgg tgtaaccggc gttgtaagaa acgtgttcat cttccagacc agaccaggtc
    15361 gggctggaga taattttgcg cggctgtgcc tgaatatcgc ggaagcggat cttctcgtct
    15421 tctttattca gcgccagatg cgtatggtca cgaccggtaa attcgctcag ggcagcccag
    15481 gctttcacgg ctacctgacc gttggtttcc ggtgccagtg tcaggatcat ctctgccgca
    15541 tcaattgcgg tgttcagcat cggctggcct ttcgccggac cttccgcttt ggtgtagttg
    15601 agcttacgca gcagatccat ctcgctctgg gtgttccagg caatcccttt accgccgtta
    15661 ccgattttct ccatcagcgg gccgatagag gtaaagcgtt cgtaagttgc cggataatcg
    15721 cgctctacga ccataatgtg cggtgcagtt ttgcctggga tcaggtcgca ttcgcctttt
    15781 ttccagtctt tcacatccag cggctgcgcc agttcagcgg cagagtcgtg ctggataggc
    15841 agcgtgacga tgtcggtttc tttacccaga tggccgacgc acacttcgga gaatttcttc
    15901 gcgatggctt tgtagatttc ccagtcgctt ttcgcttccc aggccggatc gaccgccgca
    15961 gacagcgggt gaataaacgg atgcatatcc gaagtattca tgtcgtcttt ttcgtaccag
    16021 gtcgccgtcg gcaaaatgat gtcggaatag agacaggtgc tcgacagacg gaagtccagc
    16081 gtaaccacca gatccagctt gccttccaga ccattgtcct gccagtccac ttcttccggc
    16141 ttcacgccgc cctgctgccc cagatcttta ccctggatac cgtgctccgt ccccagcagg
    16201 tacttgagca taaactcatg acctttaccg gaagaaccga gcaggttaga acgccagata
    16261 aacaggttac gcgggtggtt tttgccgttt tccggctgtt ccgcagcgaa acggatagaa
    16321 ccctctttca gggatttcac cgtatagtcc accggattca tcccggcttt ttctgcttcg
    16381 ccagcgatag tcagcgggtt agtgcctaac tgcggcgcag acggcagcca gcccatgcgt
    16441 tctgcacgga cgttaaagtc gatcagatgt ccggtatagc gggatttatc cgccatcggc
    16501 gacagcagtt cttccgcagt cacggtttca taacgccact ggctggagtg gttatagaaa
    16561 taagaggtgc tgttcatgtg acgcgccgga cgctgccagt cgagggcaaa cgccagcggc
    16621 tgccagccgg tttgcggacg cagtttttcc tgacctacat agtgcgccca gccgcccccg
    16681 ctctgaccga cacagccgca gaaaatcagc atgttgatca gaccacgata gttcatatcg
    16741 aggtgatacc agtggttcag ccccgcaccg acgataatca tcgaacgacc gtgcgtttta
    16801 tcagcgttat cggcaaattc acgggcgatg cgaataatct ggctgcgaga aacgccggta
    16861 atctgctcgg cccaggccgg ggtataagct ttcacatcgt catagctggt tgcacagtta
    16921 acgtcgttca ggccacgttc cagaccgtag tttgccagcg tcagatcata aacggtggtc
    16981 accagggcgg tgctgccatc agccagttgc aggcgtttca ccggcagttt gtgcagcagc
    17041 acgttttcca gttccacttt gttgaagtgt tccgtgccgt cgccaccaaa gtacgggaag
    17101 cctacctcag cgatctcatc ctggctaccc agcaggctca gttgcagctc ggtttcttcg
    17161 ccagttttgc cgtcgcgctg ctcaagattc catttgccct tctcgcccca gcggaagcca
    17221 atagaaccgt tcggcgcaac catttcgcca ttggtattaa aggcgacagt tttccattcc
    17281 ggattgtttt cctggcccag cgcatcaacc agatcagcag cgcgcagcat acgacctgca
    17341 gcgtagtaac cgtcgcgttc ttccagcatc accagcatcg gcatgtcggt gtagcgacgc
    17401 acatagtcgg tgaaatactg gcttgggttg tcgagatgga attcacgcag cattacgtgg
    17461 cccatcgcca gcgccattgc cgcatcggtg ccctgtttcg gtgccagcca cagatcgcac
    17521 agtttggcga tttcagcgta gtctggtgtg acggcaacag ttttggtgcc tttgtaacgc
    17581 acttcagtaa agaagtgagc atctggggta cgcgtctgcg gcacgtttga accccaggcg
    17641 atgatgtagc tggagttgta ccagtcagca gattccggta cgtcagtttg ctcgccccag
    17701 gtttgcggag acgcaggagg caggtcgcag taccagtcgt agaagcttaa gcaagtaccg
    17761 ccaatcagcg agagatagcg tgcacccgat gcgtaagaaa ccatcgacat tgccggaatt
    17821 ggcgagaaac cagcaacacg gtccgggccg tagtttttga tggtgtaaac gttagatgcg
    17881 gcgatcagtt cgttcacctc ctgccaggaa gaacgaacaa aaccaccgcg tccacgcgcc
    17941 tgcttaaagc ttttcgcttt atcggcgtct tcaatgatag aagcccatgc ctcaaccgga
    18001 tcgctatgca gcgccttcgc ttcacgccac attttcatca ggcgtttgcg catcatcggg
    18061 tatttcaggc ggttggcact gtaaagatac caggagtagc tggcaccgcg cgggcagccg
    18121 cgaggttcat ggtttggcag atccggacgg gtacgcggat agtcagtctg ctgggtttcc
    18181 caggtgacca gaccgttttt gacgtagatt ttccagctgc aggagccggt gcagtttacc
    18241 ccgtgggtag agcggacgat tttgtcatgc tgccaacgct ggcgatatcc atcctcccag
    18301 tcacggttgg tattgagaag ctggccatgc ccatcggcaa aggtttcacc cttctgtttg
    18361 aagtagcgaa accggtccag gaatttactc atcggttttc tcctgtggga gcctgtcggc
    18421 cctctgataa atcgacattg ctaatgttta agggcaaggt aacgctctga aacagcgtgg
    18481 gaattgataa cgatcaagag tgatggggat gaaaaataaa gtaaattcct aagatacccc
    18541 tttaggataa ttaacagtta cagcataaca tgctgtaaat taagacgttt tatacctgta
    18601 gttatttaaa ctattaagat ttttatgcaa atgggtatta aggagtattc cccatgggta
    18661 acatattaat ggttcttaat gaaatccggc gaaataaacc atataacatt agtatgttgt
    18721 aactaaatga cgcataaaaa aagcgcgaca tcatgccgcg caaaggataa tcaaatatta
    18781 agatttattt tttagaatgc cgaccatata ccgcccaggt aatcactacg caggcgatat
    18841 agaaaatcaa aaatactttc attgcgccga ctggcgaacc cgttaatgcc agcgagctac
    18901 cgaacgcttt cgggataaag aagccaccaa tcgcgccaat cgcagagatg aaacccaacg
    18961 ccgccgccgt gtcggttgcc gcttcacgca tcgcacgttc gtcagaaccc ccttctgctt
    19021 tcacgcgatc cattgtcagt ttacggaaga tcacggaaat catctggaaa gtggaaccac
    19081 tacccagccc agctgtcagg aacagcgcca ggaagaccgc gaagaacgcc atgaagcttc
    19141 cgccctgccc gtcagtcggt aaggtcagga acagcaggcc gctgaaaatc gccatcagaa
    19201 taaagttcac cagcgtgaca cgagttccgc ccagacggtc agataatgca ccacctgcag
    19261 aacgcgccag cgcaccaata aacggcccga agaaagcgta ttgcagaatc tgaacatccg
    19321 ggaactgcgt ttttgacagc atcgcaaagc ccgcggagaa gccgatgaag gaaccgaagg
    19381 ttgccagata cagcaggctc ataatccaca gatgaccccg tttgagtacc ggcaactgct
    19441 ccttgatgga ggctttcgag gtagcaagat cgttcatgcc aaaccacgcc gcaatggtga
    19501 agatggcaag gaacggcacc catatccagg acgcattcgc cagatacagc tcagtcccat
    19561 ccggctgttt gacgccctgg ctaccaaata ctgcgaaaat cgacagtgat accaccagcg
    19621 gagcaaccaa ctgcataacg ctaacgccca tgttgcccag accaccattc aggcccagcg
    19681 cgccaccctg cttctgtttc ggaaagaaga agctgatgtt tgccatactg gatgcgaagt
    19741 tcgcgccagc aaagccgcac agcagagaga tgatgatgaa gacgctataa ggcgtggagg
    19801 tatcctgcac ggcaaaaccc agccagacac aaggaataat cagaataccg gtgctgaacg
    19861 ccgtccagcg acgaccaccg aagataggaa ccataaagga gtatggaaca cgtaataacg
    19921 cgccagaaac cgaaggcaac gcagtcaaca taaatagctg atcggtcgta aaattaaaac
    19981 cgactttcgg taagttcacc gcaacagcgc tgaacaacat ccatacgcaa aacgccagca
    20041 gcagacaggg aacggaaatc cacaggttgc ggctggcaat acgttgaccg cgttgttgcc
    20101 agaacgcagg atcttccggt cgccaatctg taatgacagc tccagtagcc ctttcggggg
    20161 cggatgagtg actcatagac acctctgata ctcgtttcga tgtctgccac cttagtgtct
    20221 gtagctaaag gcaatttgat gtaaatcaaa cgataagata actttatcta tcattgatat
    20281 ttatcattac ccatagtgag tacagtgatt tcataaaaat tatgagattt ttcacggtgc
    20341 tgtaaaatcc ctacccttac cgatgtaaag ctactaacca cacggcaaat aaggagtaac
    20401 tctttccggg tatgggtata cttcagccaa tagccgagaa tactgccatt ccagaatgta
    20461 tcgtcacatt cattaaggtt attgctcatt taaagcctga aggaagaggt ttacatgctt
    20521 aaacgttgtc tctctccgct caccctggtt aatcaggttg cgcttattgt gttgctttct
    20581 actgctattg gactggcagg gatggcggtt tctggctggc tggtgcaagg cgttcagggc
    20641 agcgcccatg cgatcaacaa agcgggatcg ctgcgcatgc aaagttaccg tctgttggca
    20701 gcagtgccat taagcgagaa agacaagccc ttaattaaag agatggaaca aacggcattt
    20761 agcgccgagt tgactcgagc agcagaacga gacggacaac tggcgcaatt acagggttta
    20821 caagattact ggcgtaatga actcatccct gcactgatgc gtgcacaaaa ccgcgaaacg
    20881 gtgtcagcgg atgtcagcca gtttgttgcc gggcttgatc agctggtatc tggttttgac
    20941 cgcaccacgg aaatgcgcat tgagacagtg gtactggtcc atcgggtaat ggcggtattt
    21001 atggcacttt tactggtgtt cactattatc tggttgcggg cgcgactgct acaaccgtgg
    21061 cggcaactgc tggcaatggc gagtgccgtc agccatcgcg attttaccca acgcgcaaac
    21121 atcagcgggc gcaacgaaat ggcgatgctt ggaactgcgt tgaacaatat gtctgcagaa
    21181 ctggccgaaa gttatgccgt acttgagcag cgggttcagg agaaaaccgc cgggctggag
    21241 cataaaaatc agatcctctc ttttttatgg caggctaacc gccgtttgca ttcccgcgcc
    21301 ccgctgtgtg aacgcctgtc acctgtactc aacggcttac agaatttaac cctgctacgt
    21361 gatatcgaac tgcgggtgta tgacactgat gatgaagaga atcatcagga gtttacctgc
    21421 cagccagata tgacttgtga tgataaaggc tgccagctct gcccgcgcgg cgtattaccc
    21481 gttggtgatc gcggcacgac cctgaagtgg cggctggctg actctcatac gcagtacggt
    21541 attttgctgg cgaccctacc gcaggggcgt catcttagcc atgatcaaca acaactggtg
    21601 gataccctgg ttgaacaact caccgccacg ctggcgctgg atcggcatca ggaacgtcag
    21661 caacagttga tcgtgatgga agagcgtgcc accattgcgc gcgaactgca tgattctatt
    21721 gcccaatctc tctcttgcat gaagatgcag gtgagttgtt tacagatgca gggcgatgcg
    21781 ctgccagaaa gcagccgcga actgttaagt cagatccgta acgaactgaa tgcatcctgg
    21841 gcgcagttgc gtgaattgct caccacattc cgcttgcagc tcaccgagcc tggattacgt
    21901 ccggcgctgg aggcgagttg cgaagagtac agcgccaaat ttggcttccc ggtgaagctg
    21961 gattatcaat tgccgcctcg tctggtgcct tcgcatcagg caatccactt gttgcaaatt
    22021 gcccgtgagg cattaagtaa cgccctcaaa cattcgcaag cgagtgaggt cgtggtgacg
    22081 gtggcgcaaa acgataatca ggtcaaactg accgtccagg ataacggctg cggcgtgcct
    22141 gaaaatgcca tccgcagcaa tcactacggc atgataataa tgcgcgaccg tgcgcaaagt
    22201 ttacgaggcg attgccgcgt ccgccgtcgt gaatcaggtg gcaccgaagt ggtggtcacc
    22261 tttattcccg aaaaaacttt cacagacgtc caaggagata cccatgagta atcaggaacc
    22321 ggctactatc ctgctgattg acgatcaccc gatgctgcga actggcgtaa aacagcttat
    22381 cagtatggca ccagatatca ccgtggttgg cgaagcgagt aatggcgaac agggtattga
    22441 actggcggaa tctcttgatc ccgatctgat cctgttagat ctcaatatgc ccggcatgaa
    22501 cggtctggaa acgctggata aactgcgcga aaagtccctc tcagggcgca ttgtggtatt
    22561 cagcgtctct aaccatgaag aagatgtggt caccgcactg aaacgcggcg cggatggcta
    22621 tctgttaaaa gatatggaac cggaagatct gctgaaagca ttgcatcagg cagctgctgg
    22681 cgaaatggta ttaagcgaag cattaacgcc tgttctggcc gccagcttgc gcgctaaccg
    22741 tgccacgact gagcgcgatg ttaaccagtt aaccccacgc gagcgcgata ttctcaagct
    22801 gattgcccag ggtttgccga acaagatgat tgcccgccgc ctggacatca ccgaaagcac
    22861 agtaaaagtg cacgtcaagc acatgctgaa gaaaatgaag ctcaagtctc gcgtggaagc
    22921 agcggtatgg gtgcatcagg agcgcatttt ctgattacgg ttcccagcgc agttcgtcgt
    22981 ttgacaatgc gtcgaacggt tcgacaagcg ttagcgtgat ctcattggag gagacacgct
    23041 gcccctggtt atcttccacc accaccgaca atcgccagtg attgctcgcc ccttccccgt
    23101 tctgccagtc aggcatgatc agcgtccagc cctccgcgct gttggcttgt gcgcccggcg
    23161 tcaaacttaa tatctgcgta tcgccctgcc aaatcagttg ccgaataccg taacgactgc
    23221 ggatttgtaa tttcagcggc actgtttcgc caggttttag atcccacggc ggtgtcgcaa
    23281 gaaacaccgt taacgttttt cgctgtcggt actcaagagt cggtagatta tttcgctgcg
    23341 gattatcata gcgactacca cgtaacgact gactttcggc aacctcgccc gcagaaagtt
    23401 gttttttgag cggtacacca aagcggtaat taagattcag cccgaggtta ttttgattct
    23461 cgccactttc accctgttta tgctgggccg tcacagtgac taatggcaca ggggtgtaat
    23521 ttaatcccag actcaacgcg acgggattgt gataacccgt accagagtta aacaaatcaa
    23581 cacgatcacc aaaatactgt tctacgctga cactggtatt gaggtgttga tagaacggca
    23641 tgcgcatccg ggctgtcagg tcgtacccgc gcgccatccg ttgttcctgc gtggctgtct
    23701 gttcatgcca tgcagcaaac ggctgataaa agtttgccga tagtcgcaaa tattcgcccc
    23761 acgcttcggc accaaagccc gctcgctgaa gattttcatc cagcaagttg tcataaaaag
    23821 tgttataacc caccagccag ttgccgcgcg cccagcgttg cccaacgccc acattgctca
    23881 ccagcccatc atcctgctga gtaagaccaa gctggctcca ggtgagataa cgatcattat
    23941 cttgtaacgg cacaaaccag cttccacgac tgccggtaaa atgaccttcg ttatccactt
    24001 tgacgtcaac actggcattt ccccacggtg atagccacga ctctacgtgc tgattaacct
    24061 gttgactaag cgcgtcgcgg acttttccca atgcgaaagc ttttgcctgc tcgccagtat
    24121 ccagcccgtt atcattcata ctggtttcgc cgaaatcttt cacaatttca gcaaagtgtt
    24181 tttccccatc atgattttca ggcgccatgc ctaaatccgg cagaccatca ttgttattat
    24241 caaagggatt tgccgctttt tgctcgaagg tagattgtgc gttagctgta ccgcctgccg
    24301 ccagcaagag taaataaaac ggaataatgc gaggaacgaa acggctcaaa acgtcggtaa
    24361 cttccggtaa aaaatgaaaa aacatagcct taaagaataa cgattactgg caacttttgt
    24421 cgtattttct cgcgctttca ggaaaatcct taaaaatgtg accgattggt cagacgctgc
    24481 agaagcagta agatagaaga tgagtgtcta attccctgaa tacaggagta aataatgcaa
    24541 aaaatcgtga tcgttgccaa tggcgcacct tacgggagcg aatccttgtt taacagcttg
    24601 cggctggcca ttgcgttacg agagcaggag agcaatctgg atctgcgtct gttcctgatg
    24661 tctgatgcgg tcacagccgg gttgcgcggg caaaaaccag gggaaggcta caacatacag
    24721 caaatgctgg agatccttac cgctcagaat gtaccggtga aattgtgcaa aacctgtacc
    24781 gacgggcgcg ggattagtac acttcctctg attgatgggg tggaaatcgg tactcttgtg
    24841 gaactggcgc aatggacgct gtcagccgat aaagtgctca cattttaatg tttgaggggg
    24901 cgtctgacgc cccacgatga gggaagttta ctcaggcgaa tcccggccgc aacacaccat
    24961 ccggataatt ctccgccagc agttttttta ccgataccag caaatcattc agcccatcgt
    25021 cctgcattcc cagtttgata agctcctgtt ccagtgaaaa caggtattgt gcgttggttc
    25081 ccagcggacc gctcgccgcc gcaatcaacg gcgcgatgac ctgagcgcga gtgtcagatt
    25141 catattctgg atgtcgcggg tccataataa acacaatggc gtttactgtg cgtccatcat
    25201 caagatcaag ctgacaccag gttggcagat aacagccggt aatcatctcg cgcttccaca
    25261 acagggttag ttcctgctcc agcgtctctt ctggcagtcg ataggcgacg cctgtggtac
    25321 gtccgccctc ttttagtgca agcatccgtc ccggctggtg cgcagtcccg cgcccggcgg
    25381 tcaggcgcag gcaaaacgcg cgatgccatc caaccagtgt accggtgcac gactcggtaa
    25441 actccagtgc cggattccac atcaacgatc cataaccgaa gatccacacc ggtctctcat
    25501 caggtcgaca agccagcgtc gccgccagcg aagccgcccg ttgttctgct gaccataaga
    25561 gtgattcttc aatggcacca aatgccgttt tacaatcggc attcatcaag aaatcacgcg
    25621 ttatcacctt cttacacctc cgccactgca tgcttccctg cgacaacttt ttggtgtctt
    25681 ccggacattt ttactgctca ttttgaaacc actaacggga caatatgcaa tccgcggacc
    25741 acttgcaagg caacaccgca cgaatagctt taactaagct accggtttta cgatttttta
    25801 tgccatttat catcatcccc tttggcatat tcatgcttca cagcagccca ggccacttta
    25861 tgcgcggttt cttcgcgact ggcgtcatca cgccgatctt ctttatcttt atattgatcc
    25921 catgcgctgt tgaacgcttc tttatagata tcctgggcat gagacggtag aacgtgcttt
    25981 acgctttccg gcaggtcgct tttcgtttta tacggcatcg ttgactcctt ttacaaagtt
    26041 gaacattaag tgtggtcaac aatatctact cttgcaaaaa cataagaatt ttcatgtcag
    26101 actgttctta agaaacgcta aaacaaacat tgcctaaacg taatacttat ttttatgccc
    26161 caatgagtaa aaaatcgaca acggggtaaa aattcgtaaa atgttaccga tatccagaca
    26221 ctttctgtta tttttcctac ctatttttta cattttcgat aaattcatct gcaaagggat
    26281 aaccataatg tcaaatgctc aagaggcggt aaaaacccgc cacaaggaga cttcgcttat
    26341 tttcccggtt ctggcgctgg tagtgctgtt cctgtgggga agcagccaga cactaccagt
    26401 ggtcattgcc atcaatcttc ttgcgcttat tggtatttta agtagcgcct ttagtgttgt
    26461 ccgtcatgcg gacgtattag cccatcgcct gggagaacct tacggttcgc ttattcttag
    26521 cctttcagtg gttattcttg aagttagttt gatttcagct ttaatggcaa ccggcgacgc
    26581 cgcgccaacg ctaatgcgtg atacgctcta ttcgatcatt atgattgtta ccggtgggct
    26641 ggtcggcttt tcattattgt tgggcggtcg taagtttgcc acccaatata tgaatctgtt
    26701 tggtattaag cagtatttaa ttgcgctgtt ccccctggcg ataatcgtac tggtatttcc
    26761 aatggctctg cctgcggcga atttttcaac cggtcaggcg ttactggtag cattaatttc
    26821 tgcggcaatg tatggtgtat ttttgctgat ccagaccaaa acgcatcaaa gtttgtttgt
    26881 ctacgagcac gaagatgaca gtgatgacga cgacccgcac cacggtaaac cgtctgccca
    26941 tagcagcctg tggcatgcta tctggttgat tatccacctg attgccgtta ttgcagtcac
    27001 caaaatgaac gccagcccgc tggagacttt gctcgacagc atgaatgccc ctgtcgcctt
    27061 tactggcttc ctggtggcac tgttgattct gtcgccggaa ggtttaggtg cattaaaagc
    27121 agtgttgaac aaccaggtcc agcgcgcgat gaatctgttc tttggttcag tgttagcaac
    27181 aatttcgctt accgtacctg tcgtcacgct aattgccttt atgacgggta acgaattgca
    27241 gtttgcactt ggtgcgccag aaatggtggt gatggtggcc tctttagtgc tgtgccatat
    27301 ctccttctcc accggacgta ctaacgtgct caatggcgca gcgcatctgg cactgtttgc
    27361 cgcctatttg atgacgatat ttgcctgaca gttatatctc ctccgatgac ggaaaccggg
    27421 ggagataata taaattcctg cttattgtat ttcttcatac tacctggaca ggtcgttaat
    27481 cagaattgcg agcctgtacc acaacctgca cgaaaccact gcaacagctg ttgaaaagcc
    27541 ggaatgtact ctttattgca aacatttcag ggatgaagtg aagatgaatt gagacgtagt
    27601 atccacacca agccggcgtt atccggtgag gcgcaatgtt gcgggggttt tatccctggt
    27661 ggcattggtt gctggaaaga gaaaaccccc gcacgttgag aggtaaaacc tgacaacagg
    27721 gctaatcttg aaaccagaca cttcaagaat agccgcaaaa tgttgtcatt acaacaaggc
    27781 ggctatatga cgctcgcgca gtttgccatg actttctggc acgacctggc ggcaccgatc
    27841 ctggcgggaa ttattaccgc agcgattgtc ggctggtggc gtaaccggaa gtaaggtgaa
    27901 aacgggcgtc aggctgccgt gatggcaata tgcgcccgaa accggccgca ggggtaacag
    27961 cctgcggcca tttttgtctt tatcacttcc acaagtaaat attggcaacc cgagctggaa
    28021 gcatcatcca gagcactcat gaaacgactg taacagctgt tgaaaaaccg gaatgcactc
    28081 ttcattgcaa acatttcagg gatgaagtga agatgaattg agtcgtagta tccacaccaa
    28141 gccggcgtta tccggtgagg cgcaatgttg cgggggcttt atccctggtg gcattggttg
    28201 ctggaaagag aaaacccccg cacgttgaga ggtaaaacct gacaacagaa cgggggctaa
    28261 tcttgaacca tcaacaattc aagaatagcc gcaaaatgtt gtcattacaa caaggcggct
    28321 atatgacgct cgcgcagttt gccatgattt tctggcacga cctggcagca ccgatcctgg
    28381 cgggaattat taccgcagcg attgtcggct ggtggcgtaa ccggaagtaa ggcgaaaacg
    28441 ggcgtcaggc tgccgtgatg gcaatatgcg cccgaaaccg gccgcagggt aacagcctgc
    28501 ggcctttttt gtatccggca cccatgaaaa aagtcttaac gcagaacgct aagactttat
    28561 ttttcaagca aaaaagatta cttgctggta tccagttctt cgaaaccttt caccagatca
    28621 tcaatcgctt tcatctgcct gaggaacggt tccagtttag ccagcggcag cgcggatgga
    28681 ccatcacatt tcgcatgttc cggatccgga tgcgcttcaa taaacagccc cgccagacct
    28741 accgccatac cggctcgtgc cagctcagcc acctgagcac gacgaccgcc ggaagctgcg
    28801 ccaaaaggat cgcggcattg caatgcgtgg gtcacgtcga aaatcaccgg cgagttacca
    28861 gacactttct tcataatgct gaagcccagc atatcgacaa ccaggttgtc atagccgaag
    28921 ttagcaccac gatcgcaaag aatcactttt tcgttgccgc cttctttgaa tttatcaacg
    28981 atattaccca tctgtcccgg gctgacaaac tgtggtttct tgacgttaat taccgcaccg
    29041 gttttcgcca tggcttcaac caggtcagtc tggcgagcaa gaaacgccgg caactgaatc
    29101 acatccacga catcagcaac gggctgtgcc tgacttggtt cgtgaacgtc ggtgataatt
    29161 ttcacgccaa aagtctgctt caactcctgg aagattttca tcccttcttc caggcccggt
    29221 ccacgataag agtggatgga ggagcggttg gctttgtcaa aagaggcttt gaacacgtaa
    29281 gggataccca gtttctgggt cacggttacg tagtgctcgc aaatgcgcat cgccagatcg
    29341 cgagattcca gcacgttcat accgccaaac agtacgaacg gcaggtcatt tgctacgttg
    29401 atgtcgccaa tgctaaccac tttttgtttc ataggatcgc cttaaccttg agtaaaatgt
    29461 cgattaatta atgcagcaca atatgtttat gcgcgatgtt atttatttgc gcacggatca
    29521 tttcgctgat cgggtcttcc ggacactgtt caacgaaata acttaaatcg ttcaacgcaa
    29581 cgtgttcgca atccagttgc gcataaatca acccgcgatc gcgaatttca tagggatctt
    29641 ccgggttgaa ttgtaataaa gcttcgctgg tgcgtaacgc cagctccatc tgattttctt
    29701 ccatcaacga ggctttgagt gtatccagca atttgcggat tacctcaatg ttatcagctt
    29761 catcaaggtc ttcataaaac agttccgccg acgggctgat atttcccttt aaccatacgt
    29821 ccagcatatg ttcgcttaac gattcaccgt taaaaggatt aatcagccaa atttcgccat
    29881 ccggacattc aatgcgcaat atcagctgcg tagggaaaat caccggcagc agcggcaaat
    29941 cgagacgatt cgcgacccat aataaaaccg cacctaatga taccgcactg ccctgtcgat
    30001 tctttaacac ctggtccagc cataatgcat cggaaagacg ataaacgccg cgtgaggctt
    30061 taaatcccca gtcgccgtaa aacagcgcga tcagtttttc cagctgctct tctaaaggca
    30121 gaagctggct gatttcttcc ttcgccagac taacgagacg ctccagttcg tcgtaaacat
    30181 cttgcgaggg aaaatcgcgg cggattgctt cgcaagccag gatcatgcct tcgcacaatg
    30241 gcgctttatt aaattcgaaa tcagctaacg atctcatgac ttaccccagt aacggtactt
    30301 ttgtggtggc gagtttaatg atgatgtaca gcaccaccaa cgtcagcggg aaggcaataa
    30361 tgcgcgcctg ctgactgtgc atacgacgat aatcgagtgc aataaaaccc aaaacgatat
    30421 aaatgataac tccaaacagc ttttcagtca gccactgtgc ctgcccggaa aatggcagga
    30481 tgtgcgcttt agccatcaac gcaatgccgc ttaacaataa caccgtatcg acaaccggcg
    30541 gcacaatgcg cgtccagcga gcaaatgcct gtggatgctt ctgataacgt agccagaaac
    30601 gtaaggttaa tagcccaaca gaaagcgcga tactaataag atgaacacta agcagtgtag
    30661 aaaaacttgt cattgataat agcggccgag cgttacgcgc tcgttatcac catagtcacg
    30721 gcaggtttcg acgtcatgat accccgcgag gataaatgcc tgtcgcaccg cttcgccctg
    30781 ctgccagcca tgttccagaa gcagaaagcc gccggatacc agcgcgttac gcgactgttc
    30841 gatgatatgc acgatgtctg ccattccact gtctgccgca accagcgcag tgagcggctc
    30901 aaagcggaca tcgccttgtt gaagatgagg gtcctgctcg tcaatatacg gcggattgct
    30961 gacaatcatc gcaaactgct gcccggctag cgcgctaaac cagtcgcttt gcagaatgtg
    31021 gatatttttg atcgccagat gctgggcatt acgttgtgcc agggcgacag catcgggcat
    31081 acgatctaca gcggtaattt cgcagtccgg gcgctcgcga gccagcgcaa gcgcaatcgc
    31141 cccggtaccc gtcccgagat cgagaatacg gcaaggttgt tcaggcaacc gcgccagtgc
    31201 ctgctccacc agacactccg tatccgggcg cggaattaag gtcgctggcg aaacaaataa
    31261 cggcaacgac cagaattctc gcaccccggt taaatgagca atgggttcac catcgcgacg
    31321 acgtgtcagt agcgcatcaa gttgctgaca ttgttcgtca gtcagctgcg tttcaccaaa
    31381 ggcgagaata aaagtacgcc ctctgccggt aacatgctcc agcaggattt cagcatcacg
    31441 ccgcgggctt tcgctcgcct gaagttggct tattgcttca cgtaaccagt gttgatattc
    31501 cattattcct gctcggacaa cgccgccagt tggtcggcct gatgttcctg gataatcggt
    31561 tcaatcagca tatccagctt accttccatc acttcatcca ggcggtagag cgtcaggttg
    31621 atgcggtgat cggtaacgcg cccctgcggg aagttgtaag tacggttacg gtcgctgcga
    31681 tcgccactcc ccagcaggtt acgacgggta gacgcttcgg cctgttgccg ttttgccatt
    31741 tcagcagcgt ggatgcgagc accgagtaca gaaagtgctt tagctttgtt tttatgttgt
    31801 gaacgttcgt cctgacattc aacaacaatc ccggtcggca agtgagtaat acgaattgcc
    31861 gaatcggtgg tgttaacgtg ctgaccaccc gcccctgacg agcggaaagt atcaatgcgt
    31921 aaatctgctg ggttgatgtc cggcagttct gcgtcaggca gttctggcat taccgcaacg
    31981 gtacaagcag aagtatgaat acgaccctgc gattccgtag caggaacacg ctgcacgcga
    32041 tgaccgccag attcaaattt cagacgacca tacacaccat caccgctaat tttggcgatg
    32101 atctctttat aaccaccatg ttcaccctcg ctggcgctca tgatttctac ccgccagcgg
    32161 cgggcttcgg cgtaacggct gtacatacgg aacagatcgc ctgcgaacag cgctgcttcg
    32221 tcgccgccgg ttccggctcg gacttcgagg aaggcgttac gttcgtcatc aggatctttt
    32281 ggcagtaaca gaacctgtaa ttgctgttcc agttgctcgc ttttttcttt agcttcgcgc
    32341 agttcatcct gcgccatctc acgcatttca ggatcatcga gcatcatctg tgcggtttcg
    32401 atatcttcct gaacctgttg ccagtcggta aaacagcgcg aaacatcact taactgcgca
    32461 tattcgcgtg ataatgcgcg aaaacgttcc tggtcggcga tagtttgcgc gtcacccagc
    32521 aacgcctgaa cttcttcatg gcgttcatgc agggcttcca gtttggcaac gatagaaggc
    32581 ttcataggcg taaatgcacc ctgtaaaaaa agaaaatgat gtactgctac tccagcccga
    32641 ggctgtcgcg cagaatattc aggcgttcgt tatccccgtc acgggcggcc tgttgaagtg
    32701 atttcgttgg cgcatggatc aagcggttag tcagtttcca tgccagatcc tgcataatgg
    32761 cttgcgcgtc gccgccctgc tcaagggccg ctaacgcttt ggcggttaac tcatcgcgaa
    32821 cttgctctgc ctggctgcga tactcgcgaa tggtttcgct ggcgctttgt gctcgcagcc
    32881 acgccataaa ttcgctggtt tcctgagcga caatagtttc cgcctcaacc gctgcggctt
    32941 tacgctgcgc caggttgtgc gaaatgatgc tttgcagatc atcaacgcta taaagataag
    33001 cattcgccag tttgccaact tccggctcaa catcgcgcgg aacggcaata tccaccaaca
    33061 gcattggttg gttgcgacgg ctttttaatg cgcgctccac catgcctttc ccgataatcg
    33121 gtaacgggct ggcggtggaa ctgatgatga tatcggcttc gcgcagacgt tcgtcgatat
    33181 cactcagggc aatcacttcc gcgccgactt catctgccag aatttgggca cgttcgcgag
    33241 tgcggttggc gataatcatc ttctgtactt tatgttcgcg cagatgacgc gctaccagct
    33301 cgatggtttc gcccgcgcct accagcaaca ctgtgaccgt agagagcgat tcaaagatct
    33361 gccgcgccag cgtacaagcc gcaaaagcga cagacacagc gctggcaccg atatctgttt
    33421 cagtgcgtac gcgtttcgct acagagaaag atttctggaa catgcgttcc agttcgctgg
    33481 ccttcatatg acctttttgc gaatcggcaa acgctttttt aacctgaccg aggatctgcg
    33541 gctccccaag aaccagtgaa tccaggccgc tggcaacacg cattaaatgg ctaaccgcgt
    33601 cgttatcctg atgccagtag aggcttttac gcagatcttc ttcattaaga ttgtgataat
    33661 cgcaaagcca gcggattaac gcctcttgca agttgtcctg ctcttcgacg ctaagataaa
    33721 gctcagtgcg gttacacgtc gacagcacca ccccgccctg caccatcggc tgtgcgagca
    33781 gactgtccag cgcctgatca agtttgtccg gcgaaaacga tacacgttct cgcagcgata
    33841 caggtgccgt tttatggtta ataccgagcg ctaaaagggt catgtctgcg ggaaataata
    33901 ccaacgttga taaggttagt ctcggcgcat cataccgtat gcgcgagatc aataaaaggg
    33961 agcgccccct tttggagtaa ttgcccgaac ccgttagatt tctacaatta agacaacttg
    34021 aacatagacg atgactaatg gtgacgctag cattaagggt tataactgca acgtatctca
    34081 aggacttgtc atcactatgc ccctgcccga ttttcgtctt atccgcctgc taccgctggc
    34141 tgctcttgtg ctcactgcct gttccgttac cacgcccaaa ggtcctggca aaagcccgga
    34201 ttcgccacaa tggcgtcagc atcagcaaga cgtgcgcaat cttaatcagt atcagactcg
    34261 cggcgcgttc gcttatattt ctgaccaaca aaaagtgtac gcccgctttt tctggcagca
    34321 aaccggccag gatcgctacc gtctgctgct cactaaccca ttgggcagca cggaactgga
    34381 gctgaatgct caaccgggta acgtgcagtt agtcgacaat aaaggtcagc gttataccgc
    34441 cgatgacgcc gaagagatga ttggcaaatt gactggaatg ccaattccgc tcaacagctt
    34501 gcgccagtgg attttaggtt taccgggtga tgcaaccgac tacaaactgg acgaccagta
    34561 ccgcctgagc gaaattacct acagccagaa tggcaaaaac tggaaggttg tttatggtgg
    34621 ttatgacacc aaaacgcaac ctgcgatgcc agccaatatg gaactcaccg acggtggtca
    34681 acgcatcaag ttaaaaatgg ataactggat agtgaaataa tgcggacaca gtggccctct
    34741 ccggcaaaac ttaatctgtt tttatacatt accggtcagc gtgcggatgg ttaccacacg
    34801 ctgcaaacgc tgtttcagtt tcttgattac ggcgacacca tcagcattga gcttcgtgac
    34861 gatggggata tccgtctgtt aacgcccgtt gaaggcgtgg aacatgaaga taacctgatc
    34921 gttcgcgcag cgcggttgtt gatgaaaact gcggcagaca gcgggcgtct tccgacggga
    34981 agcggtgcga atatcagcat tgacaagcgt ttgccgatgg gcggcggtct cggcggtggt
    35041 tcatccaatg ccgcgacggt cctggtggca ttaaatcatc tctggcaatg cgggctaagc
    35101 atggatgagc tggcggaaat ggggctgacg ctgggcgcag atgttcctgt ctttgttcgg
    35161 gggcatgccg cgtttgccga aggcgttggt gaaatactaa ccccggtgga tccgccagag
    35221 aagtggtatc tggtggcgca ccctggtgta agtattccga ctccggtgat ttttaaagat
    35281 cctgaactcc cgcgcaatac gccaaaaagg tcaatagaaa cgttgctaaa atgtgaattc
    35341 agcaatgatt gcgaggttat cgcaagaaaa cgttttcgcg aggttgatgc ggtgctttcc
    35401 tggctgttag aatacgcccc gtcgcgcctg actgggacag gggcctgtgt ctttgctgaa
    35461 tttgatacag agtctgaagc ccgccaggtg ctagagcaag ccccggaatg gctcaatggc
    35521 tttgtggcga aaggcgctaa tctttcccca ttgcacagag ccatgcttta agccgggcaa
    35581 gctgagtttc ggtgacaacg tcaccttgtt ccagacgttg catcgcgctc tttaatacac
    35641 cgcctggaaa ggatcatgcc tggcccgcac agttttcggc agattctttc caccaatgga
    35701 cgcatgcctg aggttcttct cgtgcctgat atgaagcttt ttgctggtaa cgccaccccg
    35761 gaactagcac aacgtattgc caaccgcctg tacacttcac tcggcgacgc cgctgtaggt
    35821 cgctttagcg atggcgaagt cagcgtacaa attaatgaaa atgtacgcgg tggtgatatt
    35881 ttcatcatcc agtccacttg tgcccctact aacgacaacc tgatggaatt agtcgttatg
    35941 gttgatgccc tgcgtcgtgc ttccgcaggt cgtatcaccg ctgttatccc ctactttggc
    36001 tatgcgcgcc aggaccgtcg cgtccgttcc gctcgtgtac caatcactgc gaaagtggtt
    36061 gcagacttcc tctccagcgt cggtgttgac cgtgtgctga cagtggatct gcacgctgaa
    36121 cagattcagg gtttcttcga cgttccggtt gataacgtat ttggtagccc gatcctgctg
    36181 gaagacatgc tgcagctgaa tctggataac ccaattgtgg tttctccgga catcggcggc
    36241 gttgtgcgtg cccgcgctat cgctaagctg ctgaacgata ccgatatggc aatcatcgac
    36301 aaacgtcgtc cgcgtgcgaa cgtttcccag gtgatgcata tcatcggtga cgttgcaggt
    36361 cgtgactgcg tactggtcga tgatatgatc gacactggcg gtacgctgtg taaagctgct
    36421 gaagctctga aagaacgtgg tgctaaacgt gtatttgcgt acgcgactca cccgatcttc
    36481 tctggcaacg cggcgaacaa cctgcgtaac tctgtaattg atgaagtcgt tgtctgcgat
    36541 accattccgc tgagcgatga aatcaaatca ctgccgaacg tgcgtactct gaccctgtca
    36601 ggtatgctgg ccgaagcgat tcgtcgtatc agcaacgaag aatcgatctc tgccatgttc
    36661 gaacactaat cgaacccggc tcaaagaccc gctgcggcgg gtttttttgt ctgtaatatc
    36721 catttgtatg acctatgcct ccttcacctg ccatttagtt gacagatgat gcgctcacgg
    36781 atgaaacatt attgtgaaca aaatattttc ctcacatgtg atgcctttcc gcgctctgat
    36841 cgacgcttgc tggaaagaaa aatatactgc cgcacggttt acccgtgacc tgattgccgg
    36901 gataaccgtc gggattattg ctatcccgct ggcgatggcg ttggctattg gtagtggtgt
    36961 ggcaccccag tacggtttat ataccgcagc tgttgcgggg attgtcattg ctctgacggg
    37021 tgggtcacgc tttagcgttt ccggtccgac tgcggcattt gtggtaattc tctatcccgt
    37081 ttcgcaacag tttgggctgg caggactgct ggttgcgacc ttgctgtcgg ggatcttttt
    37141 gattctgatg ggtctggcac gctttggtcg cctgattgag tatattccgg tttccgtcac
    37201 cttaggtttc acctcgggta tcgggatcac catcggtacc atgcagatta aagattttct
    37261 cggtctgcaa atggcccatg tcccggaaca ttatctacaa aaagtcggcg cattatttat
    37321 ggcgctgccg accattaatg tgggtgatgc tgccattggc attgtgacgc taggtattct
    37381 tgttttctgg ccgcgtctgg gcattcgttt acccggtcac cttccggcct tgctggctgg
    37441 ttgcgcggtg atggggattg ttaacctgct cggcggacat gttgctacca tcggttcgca
    37501 attccactac gtcctggccg atggttctca gggtaacggt attccgcaac tgctaccgca
    37561 actggtgctg ccgtgggatc tgcctaattc agaattcacg ctaacctggg attctattcg
    37621 cacactgctg cctgcggcat tctcaatggc aatgctcggc gcaatcgaat ctctgctctg
    37681 cgccgtggtg ctggatggta tgaccgggac gaagcacaag gcgaatagcg aactggttgg
    37741 acaggggctg gggaatatca tcgctccgtt ctttggtggt attaccgcta ccgctgccat
    37801 cgcgcgttct gccgctaacg tccgtgccgg ggcaacttcc cctatctcgg cggtgatcca
    37861 ctctattctg gttattcttg ccctgctggt actggcaccg ctgctctcct ggctgccgct
    37921 ttccgctatg gcagccctgc tgttgatggt ggcgtggaac atgagtgaag cgcataaagt
    37981 ggtcgacttg ctgcgtcatg caccgaaaga tgacatcatt gtcatgctgc tgtgcatgtc
    38041 gctgaccgtg ctgtttgata tggttattgc catcagcgtg gggatcgtgc tggcatcgct
    38101 gctgtttatg cgtcgtatcg cacgtatgac tcgcctggca ccggtagtcg tagatgttcc
    38161 agacgatgtc ctggttctgc gcgttattgg cccgctgttt tttgctgctg ctgaaggctt
    38221 attcacggac ctggagtcac gtcttgaagg caaacggatt gtgattctga agtgggatgc
    38281 cgttccggta cttgatgctg gtggtcttga tgcgttccag cgttttgtga agcgtctgcc
    38341 cgaaggatgt gaactgcgcg tgtgcaacgt ggaattccag ccactgcgca ctatggctcg
    38401 cgcaggcatt caaccgatcc cgggacgcct cgcgttcttc ccgaatcgtc gcgcggcgat
    38461 ggcggattta taagtacgag attgaccagt cagcgcaaac tgactggtca gcaaactgca
    38521 ttatttgtta gctatgacgg cgattatcgc tacggcggca acgtttgtca tagtggcgaa
    38581 cccaccagta acggtcgcac acctgttcat gcccgccaac acgggcaccc gccagccaga
    38641 gaatggcacc gacgaaaata cttagcactg caccatgtgc gaaatactgg ggcatattaa
    38701 actgtggtaa ctggttgagg attgaatacc ccacgccgac caccattacc accagaccca
    38761 accccatgag cacgttaccg agtaacgaag cgtttttgcg tttcatatgt cacctccgga
    38821 actttctggg ttgtaacagg gaatacccct cttccttatg tgtaaagtat agacaacacc
    38881 ctgtgggtta tgtgcgggcg tgatcacaat tacaaccctt atttcaacaa aactttacaa
    38941 ataaacgcct gaacaccttg actttctaat agtccactca ggcaattatt acgttatctg
    39001 catttgatac gcagtttttt gtcaagcggg gccgcaacca gtaaactacg cgccagttat
    39061 gtacacactc aggacaaaaa aacgtgacga ttaaattgat tgtcggcctg gcgaaccccg
    39121 gtgctgaata cgccgcaacg cgacataatg ctggtgcctg gttcgttgac ttactggcag
    39181 agcgtttgcg cgctccgctg cgcgaagagg ctaaattctt tggttatact tcgcgagtca
    39241 cccttggagg cgaagatgtc cgcctgttag tcccgactac atttatgaat ctcagcggca
    39301 aagccgttgc ggcgatggcc agttttttcc gcattaatcc ggacgaaatt ctggtggccc
    39361 acgacgaact ggatctgcct cctggcgtcg ccaaatttaa gttgggcggt ggccatggtg
    39421 gtcacaatgg actgaaagac atcatcagta aattgggtaa taaccctaac tttcaccgtt
    39481 tacgcatcgg aatcggtcat ccgggcgata aaaacaaagt tgttggtttt gtgttaggca
    39541 aaccgcctgt tagtgaacag aagttaattg atgaagccat cgacgaagcg gcgcgttgta
    39601 ctgaaatgtg gtttacagat ggcttgacca aagcaacgaa ccgattgcac gcctttaaag
    39661 cgcaataagt cgttgtctgc ggcatttttg ccgagtgccg tgtataatag gcaaagttat
    39721 ttccatttct gcaatctgtt agcaataaca ggttgattat taagatatta aggtgattta
    39781 aatcatggga ttcaaatgcg gtatcgtcgg tttgcccaac gtcgggaaat ctaccctgtt
    39841 caacgcgctg accaaagccg gtattgaagc ggccaacttt ccattctgca ccattgagcc
    39901 gaacacaggc gtcgtaccaa tgcctgatcc tcgcctggat caactggctg aaatcgtaaa
    39961 accgcaacgt acgcttccca cgaccatgga atttgtcgat atcgccggtc tggtaaaagg
    40021 cgcatcgaaa ggcgaaggtc tgggtaacca gttcctgacc aacatccgtg aaaccgaagc
    40081 gatcggtcac gttgttcgct gctttgaaaa tgacaacatc attcacgttt ccggcaaagt
    40141 taacccggct gacgatattg aagttatcaa caccgaactg gcgctggcag acctcgacac
    40201 ctgcgaacgt gcgattcatc gcgtacagaa gaaagccaaa ggtggcgata aagacgcgaa
    40261 agctgagctg gcggtcctgg aaaaatgcct gccccagctg gaaaacgcag gtatgctgcg
    40321 cgcgctggat ttaagcgctg aagagaaagc ggctattcgt tacctgagct tcctgacgct
    40381 gaaaccaaca atgtacatcg ccaacgtcaa cgaagacggt tttgaaaaca acccatatct
    40441 tgaccaggtg cgtgaaatcg cggcgaaaga aggttctgtt gtggttccgg tttgtgctgc
    40501 tgttgaagca gacattgccg aactggacga cgaagaacgt gacgagttta tgcaggagct
    40561 tgggctggaa gagccgggtc tgaaccgtgt gatccgtgcc ggttataagc tgctgaacct
    40621 gcaaacttac ttcaccgctg gggtgaaaga agtgcgtgca tggaccattc cggttggagc
    40681 aaccgcgccg caggcagcgg gcaaaatcca tactgatttt gaaaaaggct ttatccgtgc
    40741 acaaaccatc tcgtttgaag atttcatcac ttacaaaggt gaacaaggcg cgaaagaagc
    40801 aggcaaaatg cgtgcagaag gtaaagatta catcgtgaaa gatggcgatg tgatgaactt
    40861 ccttttcaac gtctaattaa tttattgtct catgaggttt cgctaaatct catgaagagc
    40921 gcaaaagtat ataaaatcca cgcaattgcg tggatttttc atttcaccga gtcctaatca
    40981 ttcttgtaac atcctacact tttgtgctta ttgcctacag tgaaccacga gaataattaa
    41041 agacatcagt gttcagatga cggatatctc attagcagtg atataacgtc agtttttgtt
    41101 ccactacaca tcagccccct tcacagccga tcaatattga ccacgctcca atatgctggc
    41161 gagaaaagga cttacctgaa gggtaatatc ccgcagataa agtccccgaa taacgtgatt
    41221 acgtgcgatc ctggcaagat gaaaatgata accagtagca caataacaac gatagaccag
    41281 aaaacccatt gaccaaaaat gaatatcgat gttttttgct ttttcttatt agtaatgcaa
    41341 aaatggcgca tattgttttt gttatgcaga tacatcaagc gaaaatccca taactaatta
    41401 ggattattac caaaaatatt caccaaatta tttaaccgct gtttggtgta atgtttttgt
    41461 catattaata gtaacgaatt ggaataagta acgacaactt agggaataac tcgatctaaa
    41521 ctcacctttg taagggggat gttaaatata tattaatttc tgtgtttctg attttgcccg
    41581 gtaatagaga attattctca gagaagctca tcatgattgc accatacaaa tatcatgatg
    41641 ataagaggat attattcatt gacttaaaca aaatgacaaa atccatagac ccatagcctg
    41701 ggtactatat aggagtggta tgggcatcaa acaacacaat gggaatacca aagccgatcg
    41761 tctcgctgaa ttaaaaatcc gttcgccctc aattcaactg ataaaatttg gcgctattgg
    41821 tttgaatgca attctctttt cccccctgct gatagctgct gatacaggaa gtcaatatgg
    41881 caccaatatt actattaatg atggtgacag aattactgga gataccgccg atccatcagg
    41941 aaacctctat ggtgtaatga ccccagcagg aaacacgcct ggcaatatca acctgggtaa
    42001 tgatgtcacc gtcaatgtca acgacgcctc tggatatgca aaaggaatca ttattcaggg
    42061 caaaaacagc tccctgacag ctaaccgact cacagtagat gttgttggtc aaacctctgc
    42121 catcggcatt aatttaattg gtgactatac ccatgctgac ttaggcacag gcagcaccat
    42181 taagagtaac gatgacggca tcattattgg gcatagctca acactaacag ccactcaatt
    42241 caccattgaa aactcgaacg gtataggcct aaccatcaat gactatggca ccagtgtcga
    42301 tcttggaagc ggaagtaaaa tcaagaccga tggaagtaca ggtgtttata tcggtggtct
    42361 caacggcaat aacgccaatg gtgctgcgcg ttttacggcg acagacctga caatcgatgt
    42421 tcagggctac agcgccatgg ggataaacgt acagaaaaac tctgttgtcg atctcggaac
    42481 aaacagttcc attaaaacca gtggcgataa tgcacacggc ctctggagct ttggccaggt
    42541 tagcgcgaat gcactcactg ttgatgtaac tggagccgcg gccaatggcg tcgaagttcg
    42601 tggtggtaca accactatcg gtgcagatag ccatatttct tccgcgcagg gcggtggtct
    42661 cgtcaccagt ggttcagacg cgacaatcaa tttttctggc acggcagcgc aacgaaacag
    42721 catcttttcc ggcggttctt atggtgcctc ggcccagacg gcaacggctg ttatcaacat
    42781 gcaaaatacc gatattacgg ttgatcgtaa tggcagtctg gcgctgggtt tgtgggcgct
    42841 cagcggcggt agaataaccg gagacagttt ggctatcacc ggcgcggcag gagccagggg
    42901 gatttatgcc atgaccaaca gccagatcga cctcacgagc gatctggtca ttgatatgag
    42961 tacacccgac cagatggcca tcgcaacgca acatgacgat ggttatgccg ccagccgcat
    43021 caacgcctcg ggtcgtatgc ttatcaacgg tagcgttctt tccaaaggtg ggctaatcaa
    43081 tctggatatg caccctgggt cggtttggac aggttcctcc ctcagcgata atgtcaatgg
    43141 cgggaaactg gacgttgcaa tgaataacag cgtctggaac gtaacaagta attctaatct
    43201 cgacacgctg gcgctgagcc attcaactgt cgattttgcc agccacgggt caactgccgg
    43261 cacatttacc acattaaacg tagagaacct gagcggtaac agtaccttta ttatgcgtgc
    43321 tgatgttgtt ggcgagggta atggcgttaa taataaaggg gatttattga atatcagcgg
    43381 gagtagtgct ggtaatcacg tattggctat ccgcaaccag ggcagcgagg ccacaacggg
    43441 aaatgaagtt ctgacagtgg taaaaaccac tgacggtgcg gcctcgttca gcgcgtcttc
    43501 tcaggttgag ttggggggat atctgtacga tgtgcgtaaa aatggcacta actgggagct
    43561 ttacgcttcc gggacagttc cggaaccgac tcctaatcct gaaccaacac cagctcccgc
    43621 tcagcctccc atagtcaacc ccgatcctac gcctgaaccc gatcctacgc ctaaccccac
    43681 tcccacacct aaacccacca cgaccgcaga tgctggcggc aattatctca atgtcggtta
    43741 cttattaaac tatgttgaaa accgtacgct gatgcaacga atgggtgacc tgcgaaatca
    43801 gagtaaagac ggtaatatct ggctgcgcag ttatggggga agcctggact ccttcgccag
    43861 tggcaaactg agcggctttg acatgggtta cagcggtatc cagtttggtg gggataaacg
    43921 tctctctgat gtaatgccgt tgtatgtcgg tctgtatatt ggctcaacac atgcatcgcc
    43981 ggactatagc ggtggcgacg gtaccgcacg ttcagactac atgggaatgt acgccagtta
    44041 catggcacac aacggttttt acagcgatct cgttgtaaaa gcatcgcgcc agaaaaatag
    44101 tttccacgta ctggacagtc agaacaacgg cgttaacgcc aacggcactg cgaatggatt
    44161 gagcatctcc ctggaagccg ggcagaggtt caacctgacc cctactagtt atgggttcta
    44221 tatagagccg caaacccagc tcacatacag ccaccagaat gagatggcta tgaaggcgag
    44281 taatggtctc aatatacatc tgaatcacta cgaatcgctg ctggggcgtg ccagcatgat
    44341 actgggatat gacatcaccg caggcaacag ccagctgaat atctatgtga agactggcgc
    44401 tatccgcgag ttttcagggg atacagaata tcagttgaat aactcccggg agaagtacag
    44461 tttcaaaggt aatggctgga ataacggcgt gggagtcagt gcacagtata acaaacagca
    44521 cacattctac ctcgaagcgg attacacgca gggtaacctc tttgatcaga agcaagtcaa
    44581 cggaggatat cacttcagct tttaagtacg gggtagacaa gcccttaaac gatgctgaaa
    44641 aatgttagcg tgcgccggaa tgaaatatag atcactctga tgtatcagaa atcgtctctg
    44701 tctttcatac ccggcgctta aactgccctg catcaatgcc atgttgcttc attttccgcc
    44761 acagcgtagt gcggccgatt ccaagtaaag ccgacatttc ctgaatgcga ccgcctgtga
    44821 cctgggctgc gttaataatt gcctcttttt caacttccgc aaatgacaga ctggtggaaa
    44881 ggcgtgtggc gctgacatca tctgtcgcct gctcggtaaa cagatgttcc ggcaaatcac
    44941 tgacgcgaat gcgcccatta tcactactca gagcaagatt ctcgatgacg ctgtaaagtt
    45001 caaaatcgtt gccaggccat gcacaggaaa ccaggcgagc gagggcatcg tcatcaattt
    45061 tcagccgcgt agagaagcgt ttttcaagac tgcgtaattt gttattcacc agagccggaa
    45121 tgctgccacg ccgcatacgc agaggcggga tggtaatttc aaatgcatgc agcgcgtaat
    45181 acagctggcg actaaaacga ttttgttcca ccagcattgc gaggtccgcg gtcgttgtag
    45241 caatcacttt gacatcaatt ggtattaaac gccgcgcatc cagtcgcgtg ataacaccct
    45301 gcttgataac ctgaagcaaa gcagactgta actccaccgc cagatattca atcttttcaa
    45361 gaaacagcgt gccgccgtgc gccagttcca gccgactcag acggccattt tcattgtccg
    45421 tgcgatcgcc accaataaat tcttccgcca gtgccgcatc accatataac tcacaattga
    45481 cggcgatata aggacctgca gcacgctcgc tttcattatg aattgcctga cttagcagtg
    45541 ccttgcccac gccctcttct ccacaaagca ggacaggaaa gctactgcgc gccgcctggc
    45601 gaccaaaatg aatcaagcgg cgggtttgcg gatcgtcctg tggcatatga gcgaaggtat
    45661 ggctgacttt tcctaattga ctggtcatta actgccgcat ctgttccaca ggatggagca
    45721 acaaaataaa gctggttccc tgcgtttcta ttatcggttt aagggttatc accgcatcaa
    45781 taaactggtg ctggctttca aaggttgctt ctacgtgttt gagcggatgt gcctgtttta
    45841 ttgcttgttg caatacggcg ggtaacgtta agagttcagt gattgcccgt ccctgacttg
    45901 ccgtcgcgtc aaggcgcaag acccgcgccg cctgggcatt aataaattgc aaattaccct
    45961 gctcgtccca gctaatcacg ccatcatcca tactttctaa cagggcatta agttgattta
    46021 aatgacggtt agtttcagcg agcaaactgt ccgtcagcag taaatttccg acctcgcggg
    46081 cgattgccaa cgtcaacggc aaatcagctg cggtagtttg ctcaaccgga cacgccagcg
    46141 ctattgttcc cgtcaatcgg cccttgctgt caaataacgg cgttacacaa aaggcccagt
    46201 tccagagtgc ctgtttgaaa tgttgatcgg ccatcgtttt cacggcctga ccagagatag
    46261 ccgctaacga tagcgcacaa gtaccaataa ttccctcggc gcaatacgtg ccgtcattga
    46321 atcccagtgc acttagctgc tgcaaggttt gcggatcgcc attacggctg agaatgcagg
    46381 cggtttcatc gaggataaac agcgcacact ctcgcggagc catatattcc caggcatctt
    46441 ccagcgcagc ctgcccgagc gtcagcatcg ctttcttacg ccgataaata gaagcaaatg
    46501 tcacgccctg ggcctgatgt ggtacgttcc atgtctcccg tttcatcagc ttattgcatc
    46561 gctcccagga ggttgcaatt aagggagata tgccccgacc atcgttgtta aaagcgccac
    46621 tcatatcccg catcccttat gacaaaaggt gttccggctc gcaattttaa gtattaatac
    46681 acgtaaaagt ttcaatatgg aacattcatt aaagcatatg tttcactatg aaacataatt
    46741 tgggctaatt ttcttttccc ttgcctgatg cacaatggat tcatcggcag tcagtggtcg
    46801 ccgtgtcgtt gaacatcatc catgccctac cgtaattgct ggagcaaaat aatgaaaaaa
    46861 ttgatcaatg atgtgcaaga cgtactggac gaacaactgg caggactggc gaaagcgcat
    46921 ccatcgctga cactgcatca ggatccggtg tatgtcaccc gagctgatgc ccctgttgca
    46981 ggaaaagtcg ccctgctgtc gggtggcggc agcggacacg agccgatgca ctgtggttat
    47041 atcggtcagg ggatgctttc gggggcctgt ccgggcgaaa ttttcacctc accgacgccc
    47101 gataaaatct ttgaatgcgc catgcaagtt gatggcggcg aaggtgtact gttgattatc
    47161 aaaaattaca ccggcgatat tcttaacttt gaaacagcga ccgagttact gcacgatagc
    47221 ggcgtaaaag tgaccactgt ggtcattgat gacgacgttg cagtaaaaga cagtctttat
    47281 accgccgggc ggcgcggcgt tgccaacacc gtattaattg aaaaactcgt aggcgcagcg
    47341 gcggagcgtg gcgactcact ggacgcctgt gcggaactgg ggcgtaagtt gaataatcaa
    47401 ggccactcaa taggtatcgc tctcggtgcc tgtaccgttc ctgccgcggg caaaccttct
    47461 tttaccctgg cggataatga gatggagttt ggcgtcggca ttcatggtga gccgggtatt
    47521 gaccgtcgcc ccttctcttc ccttgatcaa accgtcgatg aaatgttcga caccctgctg
    47581 gaaaatggct cataccatcg cactttgcgt ttctgggatt atcaacaagg cagttggcag
    47641 gaagaaccac aaaccaaaca accgctccag tctggcgacg gggtgattgc gctggttaac
    47701 aatcttggcg caactccact ttctgagctg tacggcgtct ataaccgcct gaccacacgt
    47761 tgccagcaag cgggattgac tatcgagcgt aatttaattg gcgcgtactg cacctcactg
    47821 gatatgaccg gtttctcaat cactttactg aaagttgatg acgaaacgct ggcactctgg
    47881 gacgccccgg tccacacccc ggcccttaac tggggtaaat aaggagaaag caatgtcact
    47941 gagcagaact caaattgtta actggctcac tcgttgtggc gatattttca acaccgagag
    48001 cgagtatctt accggactgg atcgcgagat tggcgacgct gaccacgggc taaatatgaa
    48061 ccgaggcttt agcaaagtgg tggaaaaact ccctgctatt gcagataaag atatcggttt
    48121 cattctcaag aataccggta tgacgctgct ttcctgcgtc ggtggtgcca gtggtccgct
    48181 gttcggtacc ttctttatcc gcgccgcaca ggcgacccag gcacggcaaa gcctgacgct
    48241 ggaagagctt tatcagatgt tccgcgatgg cgcggacggc gtaatcagtc gcgggaaagc
    48301 cgaacctggc gataaaacca tgtgtgatgt gtgggtgccg gtggtggaat cgttacgtca
    48361 gtccagcgag caaaatctct ctgttccggc ggcgctcgaa gctgccagta gcatcgccga
    48421 attcgctgca caaagtacga ttacgatgca agcccgcaaa ggccgcgcca gttatctcgg
    48481 tgaacgcagt attggtcacc aggatcccgg cgcaacctcg gtgatgttta tgatgcaaat
    48541 gttggcgtta gccgcaaaag agtaaggaat tggtgatggt aaacctggtc atagtttcac
    48601 atagcagccg actgggagaa ggtgtcggtg aattagcccg tcagatgtta atgagtgata
    48661 attgtaaaat cgccattgct gcgggaattg acgatccaca aaatcccatt ggtaccgatg
    48721 ccgtcaaagt gatggaggcc atcgaatctg ttgctgatgc cgaccatgtg ctggtcatga
    48781 tggatatggg tagcgcatta ttgagtgctg aaactgcgct ggaattgctg gctcccgaga
    48841 tcgccgcaaa agtacgtttg tgtgctgcgc cgttggtcga aggtacactg gcagcaactg
    48901 tcagcgcggc ctcgggggcg gagatcgaca aagttatctt tgacgccatg catgcgctgg
    48961 aagccaaacg tgaacaactg ggtttaccgt cctccgacac tgaaatctct gactcatgtc
    49021 ctgcgtacga tgaagaagcc cgttctctgg cggtggtcat taaaaaccgt aacggcctgc
    49081 acgtccgtcc ggcctcccgg ctggtttata ccttatcgac atttaatgcc gatatgttgc
    49141 tggaaaaaaa cggcaaatgc gtcacaccgg agagtattaa ccagattgcg ttactacaag
    49201 ttcgctataa cgatacgctg cgcctgattg cgaaagggcc agaagctgaa gaggcactga
    49261 tcgctttccg tcagctggct gaagataact ttggtgaaac ggaggaagtt gctccaccta
    49321 ctctgcgtcc cgttccgcct gtttcgggta aagcctttta ttatcaacca gttttatgta
    49381 cggtacaggc aaaatcaccc ctgaccgtgg aagaagaaca ggaacgatta cgccaggcca
    49441 ttgacttcac gttattagat ctgatgacgt taacagcaaa agcagaagcc agcgggcttg
    49501 acgatattgc cgcaatcttt tctggtcacc atacactgtt agatgatccg gaactgctgg
    49561 cggcggcaag cgaactcctt cagcacgaac attgcacggc agaatatgcc tggcagcaag
    49621 ttcttaaaga acttagccag caataccagc aactggatga tgaatatcta caagctcgct
    49681 atattgatgt ggacgatctt ctgcatcgca ccctggtcca cctgacccaa acgaaagaag
    49741 aactcccgca gtttaactcg ccaactattc tactggcgga gaacatttat ccctccccag
    49801 tactgcaact ggatccggcg gttgtaaaag gtatctgcct tagcgccgga agtccggtat
    49861 cccacagcgc cctgatcgcc cgtgaactgg ggattggctg gatttgccag cagggtgaga
    49921 aactgtatgc gatacaacca gaagaaaagc taacgctgga cgttaaaacg caacgtttca
    49981 accgtcaggg ttaagccacc gttcggatgg catcgttctg atgtcatccg gcaatttact
    50041 cagcactaat gttttttaac tacctgttat atttttcacc aatttatgat gatttttctt
    50101 cgctaatcgc gaaccaaacg acgctggctg gctaataaac agacgttatt tgatcactct
    50161 atttcgccgt tatttatccc tttaattccc ttttctaaaa tgcctgacag ttcgcagaat
    50221 gagatttcga tcatgcagct agtgcgatcc tgaactaagg ttttctgata cttgaatacc
    50281 gttttattcg gtttcgccta aggagaatga ttgatgaaat cccccgcacc ttctcgcccg
    50341 caaaaaatgg cgttaattcc agcctgcatc tttttgtgtt tcgctgcgct atcggtgcag
    50401 gcagaagaaa caccggtaac gccacagccg cctgatattt tattagggcc gctgtttaat
    50461 gatgtgcaaa acgccaaact ttttccggac caaaaaacct ttgccgatgc cgtgccgaac
    50521 agcgatccgc tgatgatcgg atgcagcaaa accagagcgg atttgatctg cgccatttcg
    50581 ttaacgtcaa tttcaccctg ccgaaagagg gcgagaaata tgttccgcca gagggacagt
    50641 cactgcgcga acatattgac ggactttggc cggtattaac gcgttctacc gaaaacaccg
    50701 aaaaatggga ttctctgtta ccgctgccgg aaccttatgt cgtgccaggc ggacgttttc
    50761 gcgaggtata ttactgggac agttacttca ccatgttagg acttgccgaa agcggtcact
    50821 gggataaagt cgcggatatg gtggccaatt ttgctcatga aatagacact tacggtcata
    50881 ttcccaacgg caaccgcagt tactatttaa gccgctcgca gccgcccttc tttgccctga
    50941 tggtagagtt actggcgcag catgaaggcg atgccgcatt gaagcaatac ctgccgcaaa
    51001 tgcaaaaaga atatgcttac tggatggacg gtgttgaaaa cctgcaagcc ggacaacagg
    51061 aaaaacgcgt tgtaaaactt caggatggta cccttctcaa ccgctactgg gacgatcgcg
    51121 atacgccacg accagagtca tgggtggaag atattgccac cgctaaaagc aatccgaatc
    51181 gacctgccac tgaaatttac cgcgacctgc gctctgctgc tgcgtctggc tgggatttca
    51241 gctcgcgctg gatggacaat ccgcagcaac taaatacctt acgcaccacc agcatcgtac
    51301 cggtcgatct gaacagcctg atgttcaaaa tggaaaaaat ccttgcccgc gccagcaaag
    51361 ctgccggaga taacgcgatg gcaaaccagt acgaaacact ggcaaatgcc cgtcaaaaag
    51421 ggatcgaaaa atacctgtgg aacgatcaac aaggctggta tgccgattac gacctgaaaa
    51481 gtcataaagt gcgcaatcag ttaaccgcgg ccgccctgtt cccactgtac gtcaatgcgg
    51541 cagcgaaaga tcgcgccaac aaaatggcga cggcgacgaa aactcatctg ctgcaacccg
    51601 gcggcctgaa caccacgtcg gtgaaaagtg ggcaacaatg ggatgcgcca aatggctggg
    51661 caccgttaca gtgggtcgcg acagaaggat tacaaaacta cgggcaaaaa gaggtggcga
    51721 tggacattag ctggcacttc ctgaccaatg ttcagcacac ctatgaccgg gagaaaaagc
    51781 tggtggaaaa atatgatgtc agcaccaccg gaacgggggg cggcggtggc gaatatccat
    51841 tacaggatgg ctttggctgg accaatggcg tgacgctgaa aatgctggat ttgatctgcc
    51901 cgaaagagca accgtgtgac aatgttccgg cgacgcgtcc actcagtgaa tcaacaacac
    51961 agccggtaaa acaaaaagag gcggaaccta cgccttaact ttgctgtata tcgagcaatg
    52021 ccattatgtg cataaaattg gcattgctct tttttcgcca cgaaatgccc cccattctac
    52081 ggtctatgct tttattttct tatatcagag aagattttaa ttttatttta ttttatcaat
    52141 ttgttaatca gtacacctct atttatattt ttcgccactc cgccattctc cctgacaatt
    52201 ttcttgtgga acaaagtgtt atttaagcaa caagcaaatc attctgtctc tttagacaaa
    52261 ttttgacatc aaacatgatt tttttcgata tatatgtaaa tatatagcgg ttttaattat
    52321 caggatataa cttatgtcac taaccagacg gaggtttaca cagattcttg cgtcgacgtt
    52381 gttcctgcat catctgcctt cttttgcaca atcagtcaaa ttctgggcct cactgacgct
    52441 tcccgaagcc caaaacatta cacgcatcgt cagcgcaggt gcgcccgccg atttgctatt
    52501 gctggctgtc gcgccagaaa aaatggtcgg tttttcctct tttgattttg cccgtcaggc
    52561 attaattcca ttgtcagagc acattcgcca gttacccagg ctgggacgac tcgccgggcg
    52621 cgccagcaca ctctcgctgg aagggcttat ggcgttacat cccgatttgg tcgttgattg
    52681 cggcaatacg gatgaaacct ggatctccca ggcacggcag gttagcgaac agacacaaat
    52741 accctggtta ttgcttaacg ggaaactggc gcaatcagca gaacagttaa caacgcttgg
    52801 caaaacgtta ggcgaagagc atcgcgccgc agaacaagcc aatctcgcca gccgcttcgt
    52861 tggtgaagct caggcattcg ccgcctcacc cgccgctaac ctccgctttt atgctgcgcg
    52921 cggccctcga gggctggaga cgggcttaaa gggttcgttg cataccgagg cggcggaatt
    52981 attaggtttg cacaacgtcg cgcaaatagc cgatcgccaa ggcctgacac aggtttccat
    53041 ggaaaatctc ctgcgctggc agccggatat tattctggtt caggaggccg ttactgcaga
    53101 ttttattcgt cgtgatcctc tctggcatgg cgtgaaagcc gttgcagaac aacgcatctt
    53161 atttttaagt ggcttgccct ttggctggct ggatgcccca ccgggaatca accgtcttct
    53221 gggattacgc agacttcacg cctggctgga tcccgccatc aatcgccagt ttaaaagtga
    53281 catgcagcat tacgcccaac tgttctggca ttgttcactc agtgacgccg actatcaaaa
    53341 attggtggcg acctaatgag aatcgtaaac ggatgtattt tgctgctggc agctatcagt
    53401 atcacttttg ccgccgtaag tggggcttat catctcgata tgcagcaact gctggcgcta
    53461 attcttcgtc aagaaaatgt tcctgtgcag gaacaaattg tcttctggca aatccgcgtg
    53521 ccgcgtattc ttgctgcgct gttactgggc gcggcgctgg caggagccgg aaccacctat
    53581 cagggaatgt tgcgtaatcc cttagtttca ccggatattt taggcgtctc cgccggtgcc
    53641 ggacttggtg cctgcacggc aattctgtgg ggattatcca ttgtttacat ccagctgtac
    53701 gcattttgtg gcgggctgat ggtggtcgcg ggcgtctggt tgattacgcg tcgggtgacc
    53761 cgccacgatc cgatcttaac cctggtgctg gtaggcatcg cgttaggaac gttgtgcgga
    53821 gcggggatat cgctaattaa aacgctggcc gatccttaca cccagttacc gtcaattacc
    53881 ttctggttgc tggggggact gtccaccgtg accttacgcg atctctgcta tgctgcaccg
    53941 atcattctca taggctcctt gcccctgttt tttctccgct ggcgcatgaa tttactcacg
    54001 ctttccgacg acgaagcccg ttcgctggga ctgaacgtga cgcgtctgcg ctttgtattg
    54061 attgtctgcg ccacactaat caccgccagt acggtagcaa tagcgggcat aattggttgg
    54121 gtcggcctgg tcgttccgca cattgcccgc ttgttgaccg gtcataacca tcagcagctg
    54181 ttgcccatgg caatgtgtac tggcgcgatt ctgctcctgc ttaccgatac gctggcacgc
    54241 agcattggaa caacagaaat cccacttggc attctgaccg cttttgttgg cgcaccgttt
    54301 ttcctgcttt tgttgttacg cggaggtcgc cagtgacttt gcttgccgtc cgtcatgcgt
    54361 ctctgggtta ctcccgccat ccggtgctac gcgacgtgtc cttcaccctg tcgcaaggaa
    54421 caatttgttg tctgttagga gccaatggtt gcggcaaaac cacactcatg cgctccattc
    54481 ttggcgttat accgttatta aaaggcgagg tcttactgga tagcctccct gtgcagacac
    54541 tcagccatcg ccagcgcgcg caggctattg cctgggtgcc gcaggcgcac gacggtattt
    54601 ttgcttttag tgtgctggat atggtgttga tgggcctagc ccccaccata ggcgcttttt
    54661 ccgttcccgg taagcaagaa cgcctgaaag ccacggagca actggaaaag ctgggcattt
    54721 tgcatctggc ggcacgacgc tggaatacgc ttagcggcgg tgaacgtcag ctggctttaa
    54781 tcgcccgtgc actcgtccag caaccgcgct tgttgttact ggatgagcct gcctccagcc
    54841 tcgatttcgg tcatcagatc caactactgg acacgctggc acagctcaaa aataacggca
    54901 tgacaatgtt aatgtcgact catcatccgc tccatgccaa cgccattgcc gacagcatta
    54961 tccaggtaga gccagatgga cgagtaacac aaggcttacc gaccgaacag ttaacaacaa
    55021 acaaactggc tgccctgtat cgggtttctg ccgaccagat tcatcatcac ctctctgcaa
    55081 ttagccacta aggaaaaatc atgctcattg atgatattga ttttgcagac ctttatctgc
    55141 agcaactcaa gctggcccac cgcacagaaa aaacgccgga tcactgggat caacgggcgg
    55201 aaaaaatggc ggaaaattgc gcctcgccta cggacagcta cctgcaacaa ctgatcgcta
    55261 aaattgacct acaaggcgct cagaccttat tcgatatggg ctgcggaccg ggtaccgtat
    55321 cgctggcgct ggctgacaaa ctcacgacga tttatggcgt tgattacagt caggggatgc
    55381 tgaacgttgc cgcccgtcgc gccgccgcgc tgaaggcaga caatgtccac ttgatccagc
    55441 gcgcctggga agaggactgg agtgacctgc cacgctgcga tattgccgtg gcttcccgct
    55501 ccactctggt ggctgatatg cgccaggcaa tgagcaaact gaataatcag gcacggcttc
    55561 gcgtctatac gactcatctg gtcagcacct cgttcgtctc tccagccatt cagcgggcgg
    55621 ctggtcggga agtgatcgaa ttacccaact atatctttgc gctcaacgtc ctgtatcaga
    55681 tgggaattta tgcccacgtt gattttatcc gtggacaaaa ttgccaacag gataacagca
    55741 cctaggaacg ttttgagcag aacgtcagct ggagtcttgg cgcgcttaac gacgatgagc
    55801 gtgagcggtt atatcgctgg tatcaacaac aagatgctcg tgccctcgcc cctgccagcc
    55861 gcgactgggc gctcatctgg tgggatagcg taccgcagga ggcactgcga tgattttcct
    55921 ttctcaggca caaatcgatg cgttacttct ggaagatatc cagggcggtg acctgaccac
    55981 gagagcatta aatattggac accagcatgg ctatatagag ttttttctcc gtcagggcgg
    56041 ctgcgtcagc ggcgtttctg tcgcgtgtaa gatgttaact acgctgggat taactattga
    56101 tgacgcggtc agcgacggtt cacaagcgaa cgcgggtcag cggctaatcc gtgcgcaggg
    56161 taatgccgca gcacttcatc agggatggaa ggcaatccag aatgtgctgg agtggagttg
    56221 cggtgtttct gattatctcg atcaaatgct ggcgttactt cgtgaacgtt atcctgatgg
    56281 caatatcgcc tgcacccgaa aagcaattcc gggcacccgc ttactggcct cgcaggcaat
    56341 tctggctgcc ggaggactga ttcatcgtgc cggatgtgcg gaaaccatat tattgtttgc
    56401 caaccaccgc cattttcttc atgacaatca ggactggtca ggcgcaatca atcagttacg
    56461 tcgccacgca cccgagaaga aaattgttgt tgaagccgac acgccgaaag aggcaatcgc
    56521 cgcgttacgc gcgcaaccag acgtacttca gctcgacaaa tttagtccgc aacaggcaac
    56581 tgaaattgcg caaatagcgc cgtcgctggc tccccactgc acgctggctc tgaccggcgg
    56641 aattaatctg acgacactca aaaattacct cgactgcggc attcgcctct ttattacctc
    56701 cgcgccttat tacgcggcac cggctgacat taaagtcagt ctgcaacccg cagcctctat
    56761 ttaattcttt gttaaacgga atttattcta tgagactcaa aaaacattat ctctgcacag
    56821 cattatcact tgcctttacc cagcaggccg tagcggctca ggagagcgac tcgctgaccg
    56881 tatggtccag tccggtatca tcgacgacga ccaccgttct cgatcaaccc accatgaagg
    56941 ccctggataa acagaatgtc gctcaggcat taagtgtcgt ccccggcgtg gtgctgcaaa
    57001 agtcaggcag ccgcaacgaa gaacaggtaa aagttcgtgg ctttgatagt cggcaggtgc
    57061 cggtctattt cgacggtgtg cccatttatg ttccctatga cggcaacctc gatctggcgc
    57121 ggattctgac caacaatctg ggggcagtcg aagtttccaa agggtattcg tcgctgcttc
    57181 agggacctaa tcagatgggc ggagccatta atatcaccac ccagaagcca acaaaacctc
    57241 tggaagcaag tctgggatat cgccagggat ggagccgtag ccaggacaat gcctatgata
    57301 tgcatgcttc atttgccgcc agcagcgaac tggggtattt gcaagtcagc ggtagccagc
    57361 taaagcagga ttttctcggc ctgccgcatg gtgtaaataa tgatattgca ggcaaacacg
    57421 gcaagatgat taattcatcg gctgatgata aacgcggcat tgtgaagctc ggatttacac
    57481 cacgtgaaaa cgatgaatac acactgactt acattaagca ggatggtgaa aaagataacc
    57541 cgccatacag cggaaatagt ggtcaaaaat cacgctactg gcagtggcca gagtatgaca
    57601 aagaaagttt ttattatcag ggaacgaccc aactaaacga tcgttttacc ctgaaaagtc
    57661 ggctgtatcg cgacaccttt gaaaatacgc tgatgatgta caactcgctg gctgatttga
    57721 aaaataaaaa aggcagctac agccattatt ccgattacag cgacggtgcc ggattacaac
    57781 tggcagccga tgtgcgtgaa aacgatctgc tgacgtttgc cgttaactgg aaagatgacg
    57841 tacaccggga aaaaggtgcg ccgcacgccg cttacgatcg ctatgaagat cgtacctggt
    57901 cgctcgccag tgaatatcaa tgggctgctg ccgataatgt cgatgttgtg gctggaatca
    57961 gctatgactg gcgcgatagc gtagaagcga aaaaacacga gaaagatggc agcatcaccc
    58021 attatgacga caacaatcag tcagctttta actggcaggt gatggggaaa taccactttg
    58081 tcaatgaaga cacgctggcg ctttcgtact atgaccgcaa acgctttccg acgctgaaag
    58141 aacgctatac cacatccaaa cctgcgtata accagatagc gattgttaac ccgcagctca
    58201 agccggaacg cgctcgcggg gtggatttaa cctggaatgg tgccttcacg cacgactggg
    58261 gatttgaggt cagcgtttac tataaccggg tgagtgatgc catcctctcg cacaatatcg
    58321 atgccgatac cattcaaaat cagaacagcg gcacggtgga ttacagcggt ctggatgccg
    58381 gtattaaggg gaaaatcagc aatatactgg atgtaggatt gagctacgcc ctgatccacg
    58441 ctgacgccaa acgtaaagac atcggcaaga taaccgatct gccaacgcag acaatgaccg
    58501 catggatgac tctcaaaccg tgggagccgt taagcgtaac gctgtcggaa gaggcgcgtt
    58561 cctccagcta cagtaacagt gacggttcac aaaaagccgc cggttttgcg gtgacccaca
    58621 ttcgagccga ttacacctta ggtcatggct tcagcgtcaa tgcgtcggtc aataacctgt
    58681 ttgataccca atatgcctac agtgaagggt ttgttgaaga aggtcgcaat ttctgggcag
    58741 gtgtcgagta tacgttctga tgtgaaagcg gatagcggca tctgtatcgc tatccgcctt
    58801 atgaaaatct tattctcttc gcaggaggcg gaatacgccc agaacgagaa tagctcccac
    58861 caccgccacc aggaagctgt gcaaattaaa accgctgatg gagccgccaa tgccaaacat
    58921 ggtcgccagc cagccgccga ccaccgcacc gactatcccg agaatacagg tcaggaaaaa
    58981 tccaccacca tcacgccccg gcatgattag cttggcgata atgccggcta tcaggccaaa
    59041 aataatccag gcaataattc ccatgttcag gccctcgttt cgatgaatcg gacgcaagaa
    59101 atgcttacgt caacgctcta agtataggca agggcgggat cgatgctggg cgtatgtcgg
    59161 ctgatttaag aacaacatgt aaaaaatatt cacgcatttg tattaagttt tgttaactgt
    59221 gaccgataaa ccaaagacag tttgtcagtc aggagttttt ccgcgtgagt cattaccatg
    59281 agcagttcct gaaacaaaat ccgttagccg tcctgggcgt gttacgcgat ttgcacaaag
    59341 ccgcaattcc tttgcgtctc agttggattg gcgggcagct gatcagcaaa atactggtaa
    59401 taaccccgga taaactggtg ctggatttcg gcagtcaagc cgaagacaac atcgccgtgc
    59461 taaaggcaca gcacattacc attaccgccg aaactcaggg tgcgaaagtc gagtttactg
    59521 ttgaacaact acagcagagt gaatacttgc agcttccggc atttattacc gtaccgcctc
    59581 ccaccttatg gtttgtacaa cgacgccgat atttccgcat ctccgcccca ctccatccgc
    59641 cttatttttg ccagaccaaa ctggcggata acagtacgtt acgtttccgc ctgtatgatt
    59701 tgtcgttagg cggcatgggc gcattactgg aaacagcaaa acctgccggg ttgcacgaag
    59761 gtatgcgctt cgctcagatt gaagtcaaca tggggcaatg gggtgttttt cactttgacg
    59821 cccagttaat ctccatcagc gagcgcaaag tga
//
LOCUS       DAZKIP010000016        58803 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZKIP010000016 DAZKIP010000000
VERSION     DAZKIP010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58803
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.016"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..173
                     /locus_tag="ACHOUK_001206"
     CDS             <1..173
                     /locus_tag="ACHOUK_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP9896687.1"
                     /translation="TVTTIKAQNVKVNNESILLPPFSHNDITLKNNHASEYELTVVDD
                     YGNNIHSKIAAR"
     gene            186..2636
                     /locus_tag="ACHOUK_001207"
     CDS             186..2636
                     /locus_tag="ACHOUK_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFP9896688.1"
                     /translation="MLRTTRWVAAIIFLYSFPGYAEETFDTHFMIGGMKGEKVSEYHF
                     DNKQPLPGNYELDFYVNNQWRGKQDITIPESPVKPCLPKVLLTKLGVKTGNLNTEDNC
                     ILLDKAVHGGQYQWDISEHRLNLTVPQAYINELERGYVPPESWDRGIDAFYTSYNLSQ
                     YRSYDSNNNSNTASYGRFNSGLNLFSWQLHSDASYSKPDDMKGTWQSNTLYLEHGWSQ
                     ILSTVQIGENYTSSLIFDSLRFSGIRLFRDMQMLPDSMQSFTPLVQGVAQSNALITVS
                     QNGYIIYQKEVPPGPFTIADLQLSGSGSDLDVSIKEADGSVRSFLVPYSSVPNMLQPG
                     ISNFDFIAGRSKIYGVKNQEDFLEANYIYGLNNLLTLYGGTILSDNYNAITLGNGWNT
                     PLGAISFDATRSSSKLNNDITHEGTSYQVAYNKYLVQTATRFSVAAWRYASQDYRTFS
                     DHLYENDKINHQSDYDDFYDIGRKNSLSANIMQPLSNNLGNVSLSALWRNYWGRSGNA
                     KDYQFSYSNSWQRISYTFSASQSYDENDKEEERFNLFISIPFYWGDDIAKTRHQINLS
                     NSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYVNQQQQNNDTSLGTNLSWRTPIATID
                     GSYSHSKNAWQSGGSISSGLVVWPGGINITNQLSDTFAILDAPGLEGAHINGQKYNRT
                     NSKGQVVYDLMIPHRENHLVLDTANSESETELQGNRQIIAPYRGAVSYVQFTTDQRKP
                     WYIQALRPDGSPLTFGYDVLDLQENNIGVVGQGSRLFIRVDEIPTGIKVALNDEQNLF
                     CTITFQHVIDENKTYICQ"
     gene            2649..3609
                     /locus_tag="ACHOUK_001208"
                     /pseudo
     CDS             2649..3609
                     /locus_tag="ACHOUK_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
     gene            complement(3647..4546)
                     /gene="nhaR"
                     /locus_tag="ACHOUK_001209"
     CDS             complement(3647..4546)
                     /gene="nhaR"
                     /locus_tag="ACHOUK_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFP9896689.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADV
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(4612..5778)
                     /gene="nhaA"
                     /locus_tag="ACHOUK_001210"
     CDS             complement(4612..5778)
                     /gene="nhaA"
                     /locus_tag="ACHOUK_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFP9896690.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            6364..6516
                     /gene="mokC"
                     /locus_tag="ACHOUK_001211"
     CDS             6364..6516
                     /gene="mokC"
                     /locus_tag="ACHOUK_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFP9896691.1"
                     /translation="MKQHKVMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(6620..7750)
                     /gene="dnaJ"
                     /locus_tag="ACHOUK_001212"
     CDS             complement(6620..7750)
                     /gene="dnaJ"
                     /locus_tag="ACHOUK_001212"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFP9896692.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(7839..9755)
                     /gene="dnaK"
                     /locus_tag="ACHOUK_001213"
     CDS             complement(7839..9755)
                     /gene="dnaK"
                     /locus_tag="ACHOUK_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFP9896693.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            10132..10536
                     /gene="yaaI"
                     /locus_tag="ACHOUK_001214"
     CDS             10132..10536
                     /gene="yaaI"
                     /locus_tag="ACHOUK_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFP9896694.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNALAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            10562..11275
                     /gene="msyB"
                     /locus_tag="ACHOUK_001215"
     CDS             10562..11275
                     /gene="msyB"
                     /locus_tag="ACHOUK_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFP9896695.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSSYRVT
                     IPAVLQIACLRQMVSATQV"
     gene            11424..11990
                     /gene="satP"
                     /locus_tag="ACHOUK_001216"
     CDS             11424..11990
                     /gene="satP"
                     /locus_tag="ACHOUK_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFP9896696.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(12025..12612)
                     /gene="mog"
                     /locus_tag="ACHOUK_001217"
     CDS             complement(12025..12612)
                     /gene="mog"
                     /locus_tag="ACHOUK_001217"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFP9896697.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(12727..13680)
                     /gene="tal"
                     /locus_tag="ACHOUK_001218"
     CDS             complement(12727..13680)
                     /gene="tal"
                     /locus_tag="ACHOUK_001218"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFP9896698.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            13959..15389
                     /gene="yaaJ"
                     /locus_tag="ACHOUK_001219"
     CDS             13959..15389
                     /gene="yaaJ"
                     /locus_tag="ACHOUK_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFP9896699.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAIIRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMR
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSRDAWDDVSQE"
     gene            15459..16235
                     /gene="yaaA"
                     /locus_tag="ACHOUK_001220"
     CDS             15459..16235
                     /gene="yaaA"
                     /locus_tag="ACHOUK_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFP9896700.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(16388..16684)
                     /gene="yaaX"
                     /locus_tag="ACHOUK_001221"
     CDS             complement(16388..16684)
                     /gene="yaaX"
                     /locus_tag="ACHOUK_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFP9896701.1"
                     /translation="MKKMQSIVLALSLVLVAPMATQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAHHDHHGGHGPGKHHR"
     gene            complement(16898..18184)
                     /gene="thrC"
                     /locus_tag="ACHOUK_001222"
     CDS             complement(16898..18184)
                     /gene="thrC"
                     /locus_tag="ACHOUK_001222"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFP9896702.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(18185..19117)
                     /gene="thrB"
                     /locus_tag="ACHOUK_001223"
     CDS             complement(18185..19117)
                     /gene="thrB"
                     /locus_tag="ACHOUK_001223"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFP9896703.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVESAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(19119..21581)
                     /gene="thrA"
                     /locus_tag="ACHOUK_001224"
     CDS             complement(19119..21581)
                     /gene="thrA"
                     /locus_tag="ACHOUK_001224"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFP9896704.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(21607..21720)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(21662..21745)
                     /locus_tag="ACHOUK_001225"
     CDS             complement(21662..21745)
                     /locus_tag="ACHOUK_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFP9896705.1"
                     /translation="MKRISTTITTTITTTITITITTGNGAG"
     gene            complement(21959..22645)
                     /gene="yjtD"
                     /locus_tag="ACHOUK_001226"
     CDS             complement(21959..22645)
                     /gene="yjtD"
                     /locus_tag="ACHOUK_001226"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFP9896706.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLAILIQ
                     QPTKSDTTADQHQLQALRERAMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(23045..23185)
                     /gene="yjjY"
                     /locus_tag="ACHOUK_001227"
     CDS             complement(23045..23185)
                     /gene="yjjY"
                     /locus_tag="ACHOUK_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFP9896707.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            23281..23997
                     /gene="arcA"
                     /locus_tag="ACHOUK_001228"
     CDS             23281..23997
                     /gene="arcA"
                     /locus_tag="ACHOUK_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFP9896708.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(24057..25409)
                     /gene="creD"
                     /locus_tag="ACHOUK_001229"
     CDS             complement(24057..25409)
                     /gene="creD"
                     /locus_tag="ACHOUK_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFP9896709.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGIGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(25467..26891)
                     /gene="creC"
                     /locus_tag="ACHOUK_001230"
     CDS             complement(25467..26891)
                     /gene="creC"
                     /locus_tag="ACHOUK_001230"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFP9896710.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPKSGRITLS
                     AEVDQEHVALKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(26891..27580)
                     /gene="creB"
                     /locus_tag="ACHOUK_001231"
     CDS             complement(26891..27580)
                     /gene="creB"
                     /locus_tag="ACHOUK_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFP9896711.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDIVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRSL"
     gene            complement(27593..28066)
                     /gene="creA"
                     /locus_tag="ACHOUK_001232"
     CDS             complement(27593..28066)
                     /gene="creA"
                     /locus_tag="ACHOUK_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFP9896712.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            28277..29146
                     /gene="robA"
                     /locus_tag="ACHOUK_001233"
     CDS             28277..29146
                     /gene="robA"
                     /locus_tag="ACHOUK_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFP9896713.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(29143..29790)
                     /gene="gpmB"
                     /locus_tag="ACHOUK_001234"
     CDS             complement(29143..29790)
                     /gene="gpmB"
                     /locus_tag="ACHOUK_001234"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFP9896714.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            29842..30354
                     /gene="yjjX"
                     /locus_tag="ACHOUK_001235"
     CDS             29842..30354
                     /gene="yjjX"
                     /locus_tag="ACHOUK_001235"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFP9896715.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASIAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTTQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVY"
     gene            complement(30501..30827)
                     /gene="trpR"
                     /locus_tag="ACHOUK_001236"
     CDS             complement(30501..30827)
                     /gene="trpR"
                     /locus_tag="ACHOUK_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFP9896716.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(30917..32854)
                     /gene="sltY"
                     /locus_tag="ACHOUK_001237"
     CDS             complement(30917..32854)
                     /gene="sltY"
                     /locus_tag="ACHOUK_001237"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFP9896717.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            33065..34732
                     /gene="ettA"
                     /locus_tag="ACHOUK_001238"
     CDS             33065..34732
                     /gene="ettA"
                     /locus_tag="ACHOUK_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFP9896718.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(34839..35006)
                     /gene="nadS"
                     /locus_tag="ACHOUK_001239"
     CDS             complement(34839..35006)
                     /gene="nadS"
                     /locus_tag="ACHOUK_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFP9896719.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVARHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(35040..36272)
                     /gene="nadR"
                     /locus_tag="ACHOUK_001240"
     CDS             complement(35040..36272)
                     /gene="nadR"
                     /locus_tag="ACHOUK_001240"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFP9896720.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVVDGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEEDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(36293..37675)
                     /gene="radA"
                     /locus_tag="ACHOUK_001241"
     CDS             complement(36293..37675)
                     /gene="radA"
                     /locus_tag="ACHOUK_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFP9896721.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(37724..38692)
                     /gene="serB"
                     /locus_tag="ACHOUK_001242"
     CDS             complement(37724..38692)
                     /gene="serB"
                     /locus_tag="ACHOUK_001242"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFP9896722.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            38798..39439
                     /gene="ytjB"
                     /locus_tag="ACHOUK_001243"
                     /pseudo
     CDS             38798..39439
                     /gene="ytjB"
                     /locus_tag="ACHOUK_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
     gene            39470..40486
                     /gene="lplA"
                     /locus_tag="ACHOUK_001244"
     CDS             39470..40486
                     /gene="lplA"
                     /locus_tag="ACHOUK_001244"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFP9896723.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGIPHEQVCEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            40518..40781
                     /locus_tag="ACHOUK_001245"
     CDS             40518..40781
                     /locus_tag="ACHOUK_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9896724.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(40942..41661)
                     /gene="deoD"
                     /locus_tag="ACHOUK_001246"
     CDS             complement(40942..41661)
                     /gene="deoD"
                     /locus_tag="ACHOUK_001246"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFP9896725.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(41718..42941)
                     /gene="deoB"
                     /locus_tag="ACHOUK_001247"
     CDS             complement(41718..42941)
                     /gene="deoB"
                     /locus_tag="ACHOUK_001247"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFP9896726.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(42993..44315)
                     /gene="deoA"
                     /locus_tag="ACHOUK_001248"
     CDS             complement(42993..44315)
                     /gene="deoA"
                     /locus_tag="ACHOUK_001248"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFP9896727.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(44442..45221)
                     /gene="deoC"
                     /locus_tag="ACHOUK_001249"
     CDS             complement(44442..45221)
                     /gene="deoC"
                     /locus_tag="ACHOUK_001249"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFP9896728.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            45479..47028
                     /gene="yjjI"
                     /locus_tag="ACHOUK_001250"
                     /pseudo
     CDS             45479..47028
                     /gene="yjjI"
                     /locus_tag="ACHOUK_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
     gene            47000..47863
                     /gene="yjjW"
                     /locus_tag="ACHOUK_001251"
     CDS             47000..47863
                     /gene="yjjW"
                     /locus_tag="ACHOUK_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFP9896729.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAAFIKGVGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALRERG
                     VSRLIFPALYL"
     gene            complement(48077..48859)
                     /gene="yjjV"
                     /locus_tag="ACHOUK_001252"
     CDS             complement(48077..48859)
                     /gene="yjjV"
                     /locus_tag="ACHOUK_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP9896730.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGESGLDL
                     FGDDPQFERQQWFLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLSRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRVVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFDVLCELRPEPEDEIAEVLLNNTYAVFNVRG"
     gene            complement(48856..49929)
                     /gene="yjjU"
                     /locus_tag="ACHOUK_001253"
     CDS             complement(48856..49929)
                     /gene="yjjU"
                     /locus_tag="ACHOUK_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFP9896731.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAYICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(50051..50212)
                     /gene="ytjA"
                     /locus_tag="ACHOUK_001254"
     CDS             complement(50051..50212)
                     /gene="ytjA"
                     /locus_tag="ACHOUK_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFP9896732.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(50339..50944)
                     /gene="osmY"
                     /locus_tag="ACHOUK_001255"
     CDS             complement(50339..50944)
                     /gene="osmY"
                     /locus_tag="ACHOUK_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFP9896733.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGIVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(51337..52926)
                     /gene="prfC"
                     /locus_tag="ACHOUK_001256"
     CDS             complement(51337..52926)
                     /gene="prfC"
                     /locus_tag="ACHOUK_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFP9896734.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(53017..53694)
                     /gene="yjjG"
                     /locus_tag="ACHOUK_001257"
     CDS             complement(53017..53694)
                     /gene="yjjG"
                     /locus_tag="ACHOUK_001257"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFP9896735.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(53709..54155)
                     /gene="rimI"
                     /locus_tag="ACHOUK_001258"
     CDS             complement(53709..54155)
                     /gene="rimI"
                     /locus_tag="ACHOUK_001258"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFP9896736.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(54124..54537)
                     /gene="holD"
                     /locus_tag="ACHOUK_001259"
     CDS             complement(54124..54537)
                     /gene="holD"
                     /locus_tag="ACHOUK_001259"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFP9896737.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            54640..55671
                     /gene="rsmC"
                     /locus_tag="ACHOUK_001260"
     CDS             54640..55671
                     /gene="rsmC"
                     /locus_tag="ACHOUK_001260"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFP9896738.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFQHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            55861..55947
                     /locus_tag="ACHOUK_001261"
     tRNA            55861..55947
                     /locus_tag="ACHOUK_001261"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55895..55897,aa:Leu,seq:cag)
     gene            complement(55986..56222)
                     /gene="yjjZ"
                     /locus_tag="ACHOUK_001262"
     CDS             complement(55986..56222)
                     /gene="yjjZ"
                     /locus_tag="ACHOUK_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFP9896739.1"
                     /translation="MLQRTLGSGWGVLLPGFLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
     gene            56363..57151
                     /gene="fhuF"
                     /locus_tag="ACHOUK_001263"
     CDS             56363..57151
                     /gene="fhuF"
                     /locus_tag="ACHOUK_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFP9896740.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDARLAQAVRATIAEHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIREYKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(57189..57866)
                     /gene="bglJ"
                     /locus_tag="ACHOUK_001264"
     CDS             complement(57189..57866)
                     /gene="bglJ"
                     /locus_tag="ACHOUK_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFP9896741.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCET
                     SNVLHPY"
     gene            complement(57824..58549)
                     /gene="yjjQ"
                     /locus_tag="ACHOUK_001265"
     CDS             complement(57824..58549)
                     /gene="yjjQ"
                     /locus_tag="ACHOUK_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9896742.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
BASE COUNT        14338 a        15589 c        14493 g        14383 t
ORIGIN      
        1 taacggttac aactatcaaa gcacaaaacg tgaaagttaa taatgaaagt atcttactgc
       61 ctcccttttc gcataacgac attacattaa aaaataacca cgcatcagaa tatgaactaa
      121 cggtagttga tgattacggg aataacatcc atagtaaaat cgccgcgaga taacaggaaa
      181 aagtcatgtt aaggacgaca cgatgggtcg ctgctattat ttttttgtat tcttttccgg
      241 gttatgctga agaaactttt gatacgcact ttatgatcgg cggcatgaaa ggagaaaaag
      301 tttccgaata tcatttcgat aataaacaac cgcttccagg aaactacgaa cttgattttt
      361 atgtaaataa ccagtggcgt ggaaaacaag atatcacgat cccagagtca ccagtcaaac
      421 catgtctgcc taaggtgcta ctaaccaaac tgggggtaaa gaccggtaac ctcaatacag
      481 aagataattg tattttactg gataaagcgg ttcatggtgg acaatatcag tgggatatca
      541 gtgaacaccg actaaattta acagtaccac aggcgtatat caatgaactt gaaagaggtt
      601 acgttccccc ggaaagttgg gatcgcggca ttgatgcttt ttatacctcc tataatttaa
      661 gtcagtatcg ctcttatgac agcaataata acagtaatac agccagttat ggccgattta
      721 acagtggatt aaatttattt agttggcagt tacactctga tgccagctat agcaaacccg
      781 atgatatgaa aggcacatgg caaagtaata ctctttacct ggaacatggc tggtcgcaga
      841 tattaagcac agttcagata ggagaaaatt acacatcctc actcatattc gactctctga
      901 gatttagcgg aatacgctta tttcgcgata tgcaaatgct acctgattct atgcagagct
      961 ttacaccttt agtacaaggt gttgcgcaaa gtaatgcctt gataacggtt tcgcaaaatg
     1021 gctacatcat ttaccaaaaa gaggttcccc cgggaccatt tactatcgca gatttgcaac
     1081 tctcgggcag cggctctgat ctcgatgtca gcataaagga agcagatggc agtgttcgtt
     1141 cctttttggt gccctactct tccgtgccta acatgctgca acctggtatt tcaaatttcg
     1201 attttatcgc cggacgcagt aagatatatg gtgtaaagaa tcaggaggac tttctcgagg
     1261 caaattatat atatggcctc aacaatcttt tgacacttta tggcggtacg attttatccg
     1321 ataactataa tgctataact ctgggaaatg gttggaatac accgctgggt gccatctctt
     1381 ttgacgctac acgatcgagt agtaagttaa ataatgatat aacacacgaa ggtacgagct
     1441 atcaggtcgc ctataataaa tatctagtac aaacggccac tcgatttagc gtggccgcct
     1501 ggcgttatgc ttcgcaggat tacaggacat tcagcgacca tctttacgaa aatgataaaa
     1561 taaatcatca gagtgactat gacgattttt atgatattgg cagaaaaaat agcctttctg
     1621 ccaatatcat gcaaccttta tccaataatc tgggaaatgt atcattaagt gcgctttggc
     1681 ggaattactg ggggcgaagt ggaaatgcta aagattacca attcagttat tccaatagct
     1741 ggcaacgcat cagttatact ttctctgcca gccaatccta tgatgaaaac gacaaagaag
     1801 aggagcgttt taatctgttt atctccattc ctttctactg gggggatgat attgccaaaa
     1861 cacgtcacca aattaactta tcgaattcga cctcattttc caaagatggc tattcctcca
     1921 acaatactgg aattactggc atagccggtg aacatgatca gttaaattat ggtatatatg
     1981 ttaatcagca acaacaaaat aatgatacat cgcttggtac gaatttaagc tggagaactc
     2041 ccatcgccac aatagatggc agctatagcc attctaaaaa cgcctggcaa agtggtggaa
     2101 gtattagtag tggattagtt gtctggcccg gtggtattaa tatcactaac cagctatccg
     2161 atacatttgc aattctggat gcgcctgggt tagaaggcgc gcatattaat ggacaaaaat
     2221 acaaccgaac aaacagcaaa ggccaggttg tttacgacct gatgatacct catcgtgaaa
     2281 accatctggt acttgataca gcaaacagtg aaagtgaaac agaattgcag ggcaatcgtc
     2341 aaattattgc gccttaccgt ggagcagttt cttatgtgca gtttacaact gaccaacgta
     2401 agccctggta tatacaggca ctgcgtcccg atggttcgcc attaactttt ggctatgacg
     2461 tactggatct ccaggaaaac aatattggag tcgttggcca gggtagtcgc ctttttattc
     2521 gcgtagatga aattccaact ggcataaaag ttgctctcaa tgatgaacag aatttattct
     2581 gtactattac ttttcaacac gttatcgatg aaaacaaaac atatatatgc cagtaaaagg
     2641 agtttactat gaagtggctt ttattaatca cattgagtct atattctttt attgtgcaaa
     2701 gtgctccctg tggattgacc aatgtcgggg aacaacgtgg aacatatata ctcaaaccac
     2761 tgagtatgaa aggtaatctc accgccgaaa aaattgaaaa tactgggaat atcattgagt
     2821 gtgcagactt gcaagagacg accaacgcca ttaaagttca ttttttaaca cagaacgctt
     2881 taaggtttaa agtcggaagt cgtcattata ttattaattt cccctttgaa tctgacggta
     2941 aaaaaaatag cgcttccagg gaagtcatct tataatttag aagaaatgct atctgcgata
     3001 aatttcacca ttccatactc tatcgcatct gtatcagata gttccaacac cgcagatatt
     3061 tttccaggaa ctccttttcc tttgttgttc gaaattgaag tgagttcatg tacagggaat
     3121 aatactaatt gtaatagcgt ccgctttaat tttaatttaa atactatttt acaggtaaat
     3181 ttaatgacct gtggttttga agatcaatcc atcgacactg gacgatttgt actgtctcgt
     3241 atcaataatg aaaattatca gtttcatcat ataagatttg actgcatcca gggcgagcaa
     3301 ggtgaattag tttttgaacc cagtgacgtt gaatattact ttgaaccagt gacgatacag
     3361 gagtcaggaa ccagcatatt aaaaaatgag ttagaaaaca gtgaggacgg tgcgggtcaa
     3421 ataggctttc aactctccaa tgatggtacg aatgaaatcc agtatggtaa aagcaattac
     3481 tactcttttc agcatcccca tgaaggaagt aatcagattc ctctatttat tcgcccgcgt
     3541 acatatggaa acaatgtctc aagcgggcaa ataatgagcc gagtcaaaat cgtggtaatg
     3601 tacaactaat ctatctcccc caccttaact ttgggggagc tgccgattaa cgcaccgctg
     3661 gactaaaaag cgcagaataa tccgtattgc agattcgctg taccgccggg tgctgaatca
     3721 tccgctcagc aaaaatagca tggtactctt ccatcacatt ctcgacgcga ccaatttcta
     3781 cgacagtttt atcggcataa aagtcatatg cataaagcgt tggggcaacg aagattgcat
     3841 tgtgcatcgc accaaaagct ttcatcaaag cggcatcatc aaactcgcca aggatttcta
     3901 cgtttaatcc ctgggagtta aaccagttaa gtaatttgcg ccctaacatt gaacgtcgcc
     3961 caggaatcaa aagccgccgt tcttcaagac aagctgggaa cggtttttcc ggcggtggat
     4021 ttgtacacca gaaactcacg ccacattcgc caattctcac ggagaacagg ccttcctgct
     4081 gcgtagaatc tatcggacag tcggaaatga tcatatccag tttatgctgg cttaattgct
     4141 ccagcagcat ttcgtgggtg gattcgaagc agcgaagatg aatcggctcg ccttctacta
     4201 ctgcagcatt cagcacgcta ctgaccagac gtttggaaag tacatcagcc acgccaacgt
     4261 caaacaataa attggattct ttgcgatagt tcacgatatc cagcatttcc tggctcaagg
     4321 taaacatttt atcggcatag cgatagacca gctcccccag ctcgctgggc tcgagaccac
     4381 gtcccttgcg cttaaataat ttgccttgca ggcgctcttc cagcgcccga atctgtccgg
     4441 taatggtttg tggtgttaaa taaagcgcct ccgctgcgcc aaccacagaa ccttctttat
     4501 aaacatgcca gaagtaatac aagtggttgt aattgatatg agacatgctc atttctctcc
     4561 ctgataacaa tgaaaaggga gccgtttatg gctccccggt aaaccgtcct gtcaaactga
     4621 tggacgcaaa cgaacgcgta accagctgta tccaattacc gccgaagaga tagaaccgac
     4681 caggataccg agtttcgccc agttaatcag ttctggatct acgctaccaa aggccaggct
     4741 ggcaataaag atagacatag taaaaccgat accgcacagg atccccaccg ccataatttg
     4801 ctgataagtc gttccctcag gcagatgcgc cagtttcaaa cgcagcgcca accagcagaa
     4861 cagactaatc cccagcggtt tgccaatcag caagccagcg atgatcccca atggcagaat
     4921 ggaggtcaag ccatccagcg tgacgccttg cagtgaaacg ccagcattag caaatgcaaa
     4981 cagtggtaaa atcagatacg ccacccacgg gtgcaacaca tgctccagtc gcttcgctgg
     5041 agaacgccca tgcttctctt tcaaaggaat aaagaagccg acaattaccc ccgccagggt
     5101 tgcgtgaacc cccgatttca acactgcagt ccacaacact acgccaacca gaatatagac
     5161 gcccgtgcgg cgtacaccac acagattcaa taccgcgagt acagcaattg ctacagccgc
     5221 gacgccaaga gaggccatcg ataagtcatt agtgtagaac aatgcgatga taatgatggc
     5281 cccaagatcg tcgataatag ccagagccat caaaaagatc ttcagcgcta acggaacacg
     5341 acttcccaat agcgccagca caccaagtgc aaaggcaatg tcagtagccg ccgggatcgc
     5401 ccacccttcg cgggtaatcg gatcggcata gttaaaggcc agatagagta atgccggcac
     5461 aatcatccca ccaatagcag cgataactgg aaatgcggcc tggcgtaagc tggctagcga
     5521 tccttgcatc agttcacgtt taacttccag accgactaac aggaaaaata ccgccatcag
     5581 cgcgtcattt atccataaca gcatgttttt gttgatttcg agtgaaccaa cccggagctg
     5641 aaccggcgtc tccagaaagt cgtgatacca tccactggtt gcgccgctgt tggccatcat
     5701 catcgccagg atagcggcaa tgataagaat aatgcctccc gaggcatcac tgctaaagaa
     5761 tcgatgcaga tgtttcactt tttatttctc tttcaggtga atagatcgtt tttactattt
     5821 taccccgcac gaatcatcgt taaaatcaga ttatagtgta ggaataattc gttttttacg
     5881 atctatagaa gaatttgcgc catcagcggt gagatgcgtt tttgacggct gtaagcgctg
     5941 acgggtgacc ctaagccctg ataatcatag cttaaggtga ggatttctga gggattaatt
     6001 aggaaaagta ataattaatt tgttgtcagt cggcgatgtt tgttaggcca atatttcaaa
     6061 aataatccag cttgtcgtca ataattgcga tgaagagata aattaaagtt aaataattaa
     6121 atcattaaaa aattacgcca taaaaatgcg aggcgtcttc cggattagaa tcgataaaac
     6181 agaatgggtt aaagagagcg atataatagc ggcgggtgct tgaggctgtc tgtctcaggc
     6241 attagctgaa cggcagatag agaaaagccc cgagtgatat tttaccatca acccgaggcc
     6301 ccctatatgc tgaacacatg tagagtgcct cttactgacc gtaaggtcaa ggagaagaga
     6361 gcaatgaagc agcataaggt gatgattgtc gccctgatcg tcatctgtat caccgccgta
     6421 gtggcggcgc tggtaacgag aaaagacctc tgtgaggttc acatccgaac tggccagacg
     6481 gaggttgctg ttttcacggc ttacgaatcc gagtaagagc aacggcgggg agtgatcccc
     6541 gccactttac aggtgctcgc atatcttcaa cgcaccctat ttttacccag gcctgcccac
     6601 gggcaggctt ttggggaggt tagcgggtca ggtcgtcaaa aaacttcttc acaccatcaa
     6661 agaagctctt tgagcgcggg ctgttgtgct cgccggttgg gccaccgaag ctttcttgca
     6721 gctcttgcag cagctgtttc tgcttctcgt tcaggcctac cggtgtttcg acgacaacgc
     6781 ggcacagcaa atcaccctgt gcgccaccgc ggacagactt gacgccttta ccgcgcatac
     6841 ggaatagctt accggtctgg gtttcgccag gcactttcag tttgacgcga ccatcaaggg
     6901 tcggtacttc gatttcacca cccagcgccg ccatagcgaa gttgatcggg acttcgcaat
     6961 acaggttgtt gccttcacgc tcgaaaatcg ggtgctgttt aacctgaacc tgaacgtaca
     7021 gatcgcctgc cggtgcgccg tgttcacccg cttcaccttc gcccgcaaga cggatgcggt
     7081 ctccagtgtc cacccctgcc gggattttaa cggacagcgt tttgctgcgc tcaacacgac
     7141 catgaccatg acatttgttg cacggatctt tgatcagcgt accgcggccc tgacagtgtg
     7201 gacaggtctg ctgcacggca aagaaaccct ggcgcatctg cacctgacca gaaccatgac
     7261 aggtcggaca ggtctgcggc tgtgtacctg gttttgcacc gctaccgtgg caaacgtcac
     7321 actcttccag agtcggaatg cggatctctt tggtcacgcc acgtacagct tcttcgaggg
     7381 tgagctccat gttatagcgt aaatcagcac cgcgcgccgc acgttgacga ccacgtccgc
     7441 cgccaaaaat atcgccgaaa acgtcaccaa aaatatcgct gaagtctgcg ccgccgccaa
     7501 aaccgccgcc gcccatgcca ccttgctcaa acgcagcatg accatactga tcgtatgccg
     7561 cacgtttttg cgagtcggtc agaacttcat aagcttcctt gatctcttta aatttcgcct
     7621 cggcctcttt gtcaccctgg ttacggtccg ggtggtattt catggccagg cgtttgtagg
     7681 cctttttgat ttcacgctct tccgctgttt tggaaacgcc taaaatctcg taataatctt
     7741 gcttagccat cttttttaaa ttgcccctag atgaatgcac gggcggagag gaaattcccc
     7801 ttcgcccgtg tcagtataat tacccgttta tagggcgatt attttttgtc tttgacttct
     7861 tcaaattcag cgtcgacaac atcgtcatct ttcgcgttgt ttgcagaagc atcagcaccg
     7921 gcagtctgct gctgggcatg ttgctgctgg gcgatttcca tcagtttctg ggaaacctgt
     7981 gccagttcct gcattttcgc ttcgatagcg gctttgtctt cacctttcag agcagtttcc
     8041 agtgcagtca gcgcagactc gatagcagtt ttgtcgtcag ccggcagttt gtcgcctgct
     8101 tcttcaacct gcttacgggt gctgtgcagc agatggtcgc cctggttgcg agtctgtacc
     8161 agctcttcaa acttacggtc agcttcggcg ttagcttcgg cgtcgcgtac cattttctgg
     8221 atttcatctt cgttcagacc agaagacgcc ttgatggtga tcttctgctc tttaccgctg
     8281 tttttatctt tcgcggaaac gtgcaggata ccgtcagcat cgatatcgaa ggtaacttcg
     8341 atctgcggca tgccgcgcgg tgccgggttg ataccatcca ggttgaactg acccagagat
     8401 ttgttgtcag ccgcacgttt acgttcaccc tgcagcacat ggatggttac cgcagactgg
     8461 ttgtcttcag cggtagagaa cacctggctg tgcttggtcg ggatagtggt gtttttcgcg
     8521 atcagcgtcg tcatcacacc gcccatggtt tcgataccca gagacagcgg ggtaacatcc
     8581 agcagcagta cgtcttttac gtcaccagtc agaacaccac cctgaacagc agcaccgatt
     8641 gctacagctt cgtccgggtt aacgtcttta cgcggctctt taccaaagaa ctcagcaact
     8701 ttcttctgaa ccattggcat acgagtctga ccaccaacga ggataacgtc gtcgatatca
     8761 gatacggaca ggccagcgtc ctgcagcgca actttcagcg gctcgatgga acggttaacc
     8821 agatcttcaa ccaggctttc cagtttcgca cgagtcactt tgatgttcat gtgtttcgga
     8881 ccggtcgcgt ctgcagtgat gtacggcagg ttaacgtcgg tctgctgagc ggaagacagt
     8941 tcgattttcg ctttttctgc cgcttctttc aggcgctgca ttgccagcgg atcgttgcgc
     9001 aggtcaatgc cctgatcttt cttgaattct tcaaccagat agttgatcag acggctgtcg
     9061 aagtcttcac cacccaggtg ggtatcaccg ttggttgcca gaacttcgaa ggttttttcg
     9121 ccgtcaactt cgtcgatttc gataatagaa atatcgaaag taccaccacc caagtcataa
     9181 accgcgatag tacggttgcc agtacctttg tccagaccgt aagccagcgc agctgcggtc
     9241 ggttcgttga tgatacgttt tacttccaga ccagcgatac ggcctgcgtc tttggttgcc
     9301 tgacgctgag catcgttaaa gtatgccggt acggtgataa cagcttcagt taccggttca
     9361 cccaggtaat cttcagcggt tttcttcatt tttttcagca cttcagcaga aatctgcggc
     9421 ggtgccattt tctggccttt aacttcgacc catgcgtcgc cgttatcagc agcaataatt
     9481 ttgaacggca tgatggaaac atcacgctgt acttcttcgt cctggaagcg gcggccaatc
     9541 aggcgtttaa tcgcaaacag ggtgttttgc gggttcgtca ctgcctgacg tttagccggc
     9601 tgaccaacca gagtttcacc atcctgggta taggcaatga tagaaggcgt ggtgcgatcg
     9661 ccttcggcgt tctccagcac acgaggagtg gtgccatcca taatcgctac acaagagttg
     9721 gtagtaccca ggtcgatacc aattatttta cccatctaaa cgtctccact atatattcgg
     9781 tcatcatgtg gttgtgagtc tgtaataggg gcgaaacgtc tggtttcaac tgcccaattt
     9841 catttttttg cagactcact cactgcggtt gactactaaa tggggtcgta acccacgtca
     9901 tcaaggggga gatgcaaaaa ttttttgtgc ttttgcgcag aaatttcgcg atatggcagg
     9961 acaagacgta aaattgacag caattttatt catataaaga atgaatcgca gtcaaggtat
    10021 tcatcaaaaa attgtaataa aaagaaaaga ttacgtgcct gaatcttctc tttatcagca
    10081 gtaaactagt gggtattcat ccccctacct cttcccacta agagaatcct tatgaaatcc
    10141 gtttttacga tttccgccag cctggcgatt agcctgatgc tgtgctgcac ggcgcaggca
    10201 aacgaccata aactcctcgg cgtcattgca atgccgcgta acgaaaccaa cgctctggcg
    10261 ctgaaacttc ctgtttgtcg cattgtgaaa cgcatacaac tctctgccga ccatggcgat
    10321 ttacagttaa gcggtgcatc ggtttatttc aaagccgccc gtagcgccag tcagagcctg
    10381 aatattcctt cagaaataaa agaagggcaa accactgact ggatcaacat taacagcgat
    10441 aacgacaata aacgctgcgt ctcaaaaatc accttttccg gtcatacggt gaactcatcg
    10501 gatatggcca cgctgaaaat tatcggcgac gactaacgcc agaatataga agccacaaaa
    10561 aatgaatgtt aattacctga atgattcaga tctggatttt ctccagcatt gtagtgagga
    10621 acagttagca aatttcgccc ggctgctcac ccataatgaa aaaggcaaaa ctcgcctctc
    10681 cagcgtactg atgcgcaacg aactgtttaa atcgatggaa ggacatcccg agcaacatcg
    10741 ccgcaactgg cagctgattg ctggagaatt acagcatttt ggtggcgata gtatcgccaa
    10801 caaactgcgc ggacacggta aattgtatcg ggccattttg ctcgatgttt caaaacgatt
    10861 gaagctgaaa gccgacaaag agatgtctac gtttgaaatt gagcaacagt tactggaaca
    10921 atttctgcgt aatacctgga agaaaatgga cgaggaacat aagcaggagt ttctgcacgc
    10981 ggtcgatgcc agggtgaatg agctggaaga gctgctgccg ctgctgatga aagacaaatt
    11041 attggcaaaa ggcgtgtcgc atctgctttc cagccaactg acccgcattt tacgcaccca
    11101 cgcagcaatg agcgtacttg ggcatggttt gctgcgcggc gcggggctgg gaggccctgt
    11161 aggcgcggca ctaaatgggg ttaaagcggt cagcggcagc tcctatcgcg tgacgattcc
    11221 agccgtactg caaatcgcct gcctgcgcca gatggttagc gccactcagg tctgataccg
    11281 gaaaccgggg aaaattttca ttttcccctc aaaagatcgt agacactgcc ccactggctg
    11341 attattatgc cgcgccctga aaacactaca gttattcagg gaaattattt caccattcat
    11401 tcgatgatga tttttgagga attatgggca acactaagtt ggctaatccg gcaccgctgg
    11461 gcctgatggg cttcggcatg accaccattc tgcttaacct gcacaacgta ggttatttcg
    11521 ctctggacgg tattattctt gccatgggca ttttctacgg cggcatcgcg caaatttttg
    11581 ctggtctgct ggagtacaaa aaaggcaaca ctttcggttt aaccgcattc acctcttacg
    11641 gttctttctg gctgacgctg gttgcgattc tgctgatgcc gaaactgggt ctgaccgatg
    11701 cgccaaatgc acagttcctt ggtgtctacc tgggtctgtg gggcgtattt acgctgttta
    11761 tgttcttcgg cacgctgaaa ggcgcacgcg ttctgcaatt cgttttcttt agcctgaccg
    11821 tgctgtttgc cctgctggcg atcggtaaca ttgccggtaa cgccgcaatc atccactttg
    11881 ccggctggat tggtctgatc tgcggtgcca gcgcaatcta tctggcgatg ggtgaagtac
    11941 tgaacgagca gtttggtcgc accgttctgc cgattggtga atcccactaa ttgtcttgag
    12001 atccccccgc tcggggggat ttttttattc gctaacgtcg cgtcttgcac tcttcggtct
    12061 gaatgcagca accacttctg gtgccgtttc aacgtatggc ccttccagca actgaatgca
    12121 gtacggtacg ctggcaaaaa taccgtgtac cacaacgtta ccctcagcgt ccttcacacc
    12181 ttccagcgtc tctttaatag acttcggctg accgggtaag ttaaggatca gcgcctgttt
    12241 gcgaatcaca cccacctgac gcgaaaggat cgcagttggt acaaaatgca ggctgatctg
    12301 gcgcatctgt tcaccaaagc caggcatctc gcggtccgct actgccagcg tcgcatcggg
    12361 cgttacgtca cgacgcgccg ggccagttcc gcccgtggtg agcaccagat ggcaactcat
    12421 ttcatccacc agctcacaca acgtttgctc gatgatcgcc tgctcatcgg ggattaagcg
    12481 ggtttccagt tcaaacggcg tggttagcgc cgatgtcagc cattcttcca gcgcagggat
    12541 acctttatcc tgataaacgc cgctggatgc gcgatcagag atggaaacta agccaatacg
    12601 taaagtattc atcaagattt ttccgtttaa acagtctcgt taaacagaat gatacactgc
    12661 gaagggagtg acagacaggc ttcagaagag gtagcgtgac cgacttcccg gtcacgctaa
    12721 gaatgattac agcagatcgc cgatcatttt ttccagtttt tcctggtcaa tagcaaactt
    12781 acggatacct tccgccagtt tatctactgc cattggatcc tggttgtgct gccacaggaa
    12841 ctcggactca gtgatacgcg ccggacgcgc tttcacttcg ccggtgtaag acagtttacg
    12901 ttcgatagcc ccttcgctct cagccagctc tttcagcagt gccggtgcga tggtcagacg
    12961 gtcgcagcct gccagttcca gaatttcgcc gatgttacgg aagcttgcgc ccataaccac
    13021 ggtttcataa ccgtgctctt tgtagtactg gtagatttca gatacagaaa ccacgcccgg
    13081 atcttctgcc ggagcgtact ctttcttatc ggtattcgct ttgtaccagt caagaatacg
    13141 gccaacaaac ggcgagatca ggaacacgcc cgcttccgca caagcacgag cctgagcgaa
    13201 ggagaacagc agggtcaggt tacagttgat accttctttt tccagctgtt ccgcagcacg
    13261 gataccctgc caggtagaag ccagtttgat cagaatacga tcgttgctaa tacctgcatc
    13321 gttgtagagt ttgatcaggc gttttgcttt cgcaattgac gcttctgtgt cataggaaag
    13381 acgcgcatca acttcagttg agatacggcc cggaaccagt ttcaggattt ccagaccaat
    13441 atttactgcc agtttgtcag tcgcgtccac gatctgctgc gcgcgatcgt tgctctgctg
    13501 tttcgcccag gcgacagcat catcaatcaa cttacggtat tccggaatct gcgctgcgtt
    13561 aagaatgaga gaagggttgg ttgtggcatc ctgcggttga tacagcttca ttgccgcgat
    13621 gtccccagtg tcggccacta cggtggtgta ctgacgaagg gaggtcaatt tgtccgtcat
    13681 gatagtattt ctctttaaac agcttgttag ggggatgtaa ccggtctgcc ctgatgatat
    13741 cacgacgcaa cgtcagcgca accgccgaca agacggttat cgccaggcga gactgtttcg
    13801 gatttctgac actgcgttga tatcgcccgc catttttaaa caaaacctca tgtatgctac
    13861 gcagaagtta tcaagtacct cgtagcgtat atacttctta aacaattggt aacgtttaca
    13921 caggaaagtc atcgcgaccg gcaataagag ggatatgcat gccagatttt ttctccttta
    13981 ttaacagcgt cctttgggga tcggtaatga tttacctgct cttcggcgca ggttgttggt
    14041 tcacttttcg caccggattt gtgcagtttc gctacatccg ccagtttggc aaaagtctta
    14101 aaaatagcat tcatccacag ccaggcggtt taacctcatt tcagtcattg tgtaccagtc
    14161 ttgcggcgcg cgtgggtagc ggcaacctgg ccggcgttgc gctggctatt accgccggtg
    14221 gacctggagc cgtcttttgg atgtgggttg ccgcgtttat cggcatggcg acctcgtttg
    14281 ccgaatgttc ccttgcacaa ctttataaag aacgtgacgt caatgggcag tttcgtggcg
    14341 gaccggcatg gtatatggcg cgcgggctgg ggatgcgctg gatgggcgtt ctgttcgccg
    14401 tctttttgct catcgcctac ggcataattt tcagcggagt tcaggcaaac gctgttgccc
    14461 gagctctgag tttttctttt gattttcccc cgctggtgac aggcattatt cttgctgtct
    14521 ttgctctgct ggcaatcatt cgcggtcttc atggcgtcgc ccggctcatg caggggtttg
    14581 tcccgttgat ggcgataatc tgggtactga ccagcctggt gatttgcgta ataaatatcg
    14641 ggcaacttcc ccacgtcatt tggtctattt ttgagagtgc ttttggctgg caggaagcgg
    14701 caggcggcgc ggcgggatat accttaagcc aggcgattac taacggtttt cagcgcagta
    14761 tgttttccaa tgaggcggga atgaggtcga cgccaaacgc ggcagcggca gcggcgtcct
    14821 ggcctccgca tccggcagcg caaggaattg tccagatgat tggcattttt atcgacaccc
    14881 tggtcatctg tacggcaagc gccatgctga tattactggc gggtaacggc acaacctaca
    14941 tgccgctgga aggtattcag cttatccaga aggcgatgcg ggtgctaatg ggttcctggg
    15001 gtgctgaatt tgttaccctc gtggttattc tgtttgcctt cagctccatc gttgccaact
    15061 acatttatgc cgaaaacaat ctcttctttt tacgcctgaa caaccctaaa gcgatctggt
    15121 gtttgcggat ctgcaccttc gcaacggtca tcggcggcac cttgctaagt cttccgctga
    15181 tgtggcaact ggcagatatc ataatggcct gcatggctat taccaatttg actgccatat
    15241 tactgctctc gcctgtggtt cataccattg ccagtgatta tctacgccag cgtaaactcg
    15301 gcgtgcgccc ggtgtttgat ccgttgcgtt atccggatat cggtcgccag ctttctcggg
    15361 acgcgtggga tgatgtttcg caggagtaat cacaactatc gatcaactca ttctcttttt
    15421 ttgctaaagt cggcataaat ttcctgcaag gactggatat gctgattctt atttcacctg
    15481 cgaaaacgct tgattaccaa agcccgttga ccaccacgcg atatacactg ccggagctgt
    15541 tagacaattc ccagcagttg atccatgaag cgcggaaact gacgcctccg cagattagca
    15601 cgctgatgcg tatcagcgac aaactggcag gtatcaacgc cgctcgcttt catgactggc
    15661 agccagattt cacgccggag aatgcccgcc aggcgattct ggcgtttaaa ggtgatgtct
    15721 acaccggctt gcaggccgaa accttcagcg aagacgattt cgattttgcc caacagcatt
    15781 tgcgaatgct ttccggcttg tatggcgtac tccgcccgct cgatttaatg cagccttatc
    15841 gtctggaaat ggggatccgt cttgagaatg cccgagggaa agatctgtat caattctggg
    15901 gagatatcat caccaacaag ctgaacgagg cgctcgcagc acaaggcgat aatgtggtga
    15961 ttaatctggc gtcagatgag tattttaaat cggtgaagcc gaagaaattg aatgccgaga
    16021 ttatcaagcc ggtgttcctc gatgagaaga acggcaaatt taagataatc agcttctacg
    16081 ctaagaaagc acgcggtctg atgagtcgtt tcattattga aaatcggctg accaaaccag
    16141 agcaactgac tggttttaat agcgaaggtt acttctttga tgaagcttcc tccagcaatg
    16201 gcgaactggt gtttaaacgc tacgagcagc gttaagtttg tagtcaatgc cggatgcggc
    16261 gtgaacgcct tatccggcct acaaaagcat gcagattcaa tatattgcag aattcacgta
    16321 ggcctgataa gacgcgccag cgtcgcatca ggcgctgaat gccggattgt tacccggcat
    16381 ttgtcattta gcggtgatgt ttgcctggac catgaccgcc gtgatgatca tgatgagctt
    16441 tcttatggtg acgcggcggt ggcggcggtc cgtgtgggtg ccagcgattg cctcgccatt
    16501 cataatgttg tttccaccag ccgtggtcgc gccagtgacc gccatcccag taataaccgc
    16561 gattatcacg atcgcctatc tgtaatttta ctgacgggac taacgtaatt tccgcagcct
    16621 gcgttgccat gggagcgacc agaaccaggg aaagtgcgag tacgatagat tgcatctttt
    16681 tcacaggtta ctccttttat cattgcccgt tatgggtcga tatccggaga ttacgcgatg
    16741 agagcggcgg ttgttattct ccgcaatcct aatctctaag ttaccgcatt tattgagaat
    16801 ttctcctttt tccctgtcgt ttctctccat attttcttca caaaaaagcc gggctgcata
    16861 aaagcaaacc cggcctgatt gagataatga atagatgtta ctgatgattc atcatcaatt
    16921 tacgcaacgc agcaaaatcg gcgggcagat tatgcgaaag caagggtaaa tcggcacgtt
    16981 ctgccagctc ttttggcaga tccaacgttt caccgagaat cgcttccacg ctctctttaa
    17041 atttcgccgg atgcgcggtg ccgaggaaca agccatattc gcctggattc aactggtcac
    17101 gcagcgcacg atacgctacg gcagcgtgcg gctccgaagt gtagcccagt tcttttaact
    17161 cacgcattgt ctgttgcgtg gtttcatcat ccacggcggc ataacccagc tctttcagtt
    17221 gccagatttt gcggcggaac aactcttcca cacgcggcca gttgttcggc tgactcacgt
    17281 ccatcgcgtt ggataacgtc gcctgagtcg ctttgggtga ccactgaccg tcatgcagga
    17341 aacgtggcac ggtatcgttc acgttggtcg cagcaataaa acgtttcacc ggcagaccga
    17401 gtgacttcgc cagcagaccc gccgtcaaat cgccgaagtt tccgcttggc accgagacaa
    17461 ccagctggtt gcgcgcttcc tgcggcagct gcgcaactgc ttcaaagtag tagcaaatct
    17521 gcgccagcaa ccggctaatg ttaatcgagt tagctgagtt taaccccagc gccactttca
    17581 gctcttcatc atcaaacgcc tgcttcacca gcgcctgaca ggcatcgaaa tcgccgtcga
    17641 tggcaacagt ttcgatattg ccgcccaatg tacagaacag tttttcttgc agtggactga
    17701 ttttgcctcg tggataaagg ataaccactt tcacattcgg taaaccgtag aaagcatgag
    17761 ccactgccgc tccggtatca ccggaggtcg cggtcagaat ggtcactggc ttatcgcccg
    17821 caatatgggt cagcatttgt gccataaagc gaccgccgaa atctttaaat gccagcgttg
    17881 gcccgtggaa caattccaga caaccgacat cgctttcaac attggcgacc ggagccggga
    17941 aggcaaacgc cgcgcgcacg cgctcttcca ggatttcctg cgggatttca tcaccaataa
    18001 acgccgagag gatcttcgca ctgcgggtga caaaatccag cttcagcatc tcatcaattt
    18061 cagtcaggct gaattccggc aggtcgtgcg gaaaaaacag cccctgattt ttgcccaacc
    18121 cctgggttac ggcttgcgca aagctgacct gctcattgtg atctttaaga ttgtagagtt
    18181 tcatttagtt ttccagtact cgtgcgcccg ccgtatccag ccggcaaata tgaacaaaac
    18241 cttcctgatt ttgcaggtag ttcttaccca accagtcggc aacgcgctgg gcggtatccg
    18301 gcttgtcaca cagagcaaac aaggtcgggc cggagccgga gataccgctc gctaccgcgc
    18361 cgatttccgc gaccgcctgc cgcgcctgcc ggaagccagg cagtaaccgt tcacggtagg
    18421 gttctgcgat aacatctttc atcagcttcg cggcaagctc aggctgacgg gaatagcagg
    18481 cgtgaatgaa gcctgccaag tgtcgcccgt gcgcaatgca atcctggcgg cgatactgcg
    18541 ccggtaaaat agcccgggct tctgccgtcg agactttaat ccccggatac gccagcaccc
    18601 acagccactc atcaaaccct ggcacttgct ggctgatgat gtcgttttct tcgatcatca
    18661 actgcatacc accaagaaaa cacggtgcca cgttgtcgta atgaatgctg ccggagattc
    18721 gtccttccag ctcgcccatc aaagccagca aacgggtgtc attaagcggc ttgccgcagt
    18781 gttcattcat cgccatcaga gccgcgacca ccgaacaggc gctggagcct aagcccgaac
    18841 cgatcggcat attcttttcc agagtcatcg ccactggaat ttgcttgccc agctcctggc
    18901 aaaaacgctc ccagcactga taaacgatat tttcccgtgg ttctgacggc agcttatcgg
    18961 caaagcgtcc gaggttgttg agactgaatg tctctgccga ctcaaccgtg actacatctc
    19021 cgagcaatgc accatcaacg ggtgtcaccg ccgccccgag cacatcaaac ccgacgctca
    19081 tattggcact ggaagccggg gcataaactt taaccatgtc agactcctaa cttccatgag
    19141 agggtacgta gcagatcggc aaagacaccg gcagcggtaa cgtcattgcc cgcaccatat
    19201 ccgcgcagca ccaacggcag cggctgataa tagtggctat aaaaggccag ggcgttttcg
    19261 ccatttttca ctttgaacag cggatcatta ccatccactt cggcaatctt cacgcggcag
    19321 acgccatctt catcaatatt gccaacatag cgcaaaactt ttccttcatc acgggccttc
    19381 gccacgcgcg cggcaaagag atcgtcgagc tgtgacagat tcgccataaa agcggcaaca
    19441 tcaccctcag cgttaaactc tgcgggcagc acaggttcaa tttcaatatc cgccagctcc
    19501 agttcacgtc ccgtttcacg agcgagaatt aatagtttac gcgctacatc cataccagaa
    19561 agatcatctc gcggatccgg ttcggtataa cccatttccc gcgccagcgt agtcgcctcg
    19621 gagaaactca tgccttcgtc taacttgccg aagatataag aaagcgaacc tgaaagaatg
    19681 ccggagaact tcatcaattc atcaccagca ttgagcagat tttgcaggtt ctcaataacc
    19741 ggtaatccag ccccaacgtt ggtgtcatag aggaatttac gccgcgattt ttcagccgca
    19801 tgacgcaaca gatggtagta atccatcgac gaggtgttgg cctttttgtt cggcgtgaca
    19861 acgtggaaac cttcgcgcag gaagtcggca tattgatccg ccactgcctg gctggaggtg
    19921 cagtcaacaa tgaccgggtt cagcagatga tattctttca cgaggcgaat taagcgcccg
    19981 agattaaacg gctctttggc ttgcgccagt tcttcctgcc agttttccag atttaggcca
    20041 tgcacattgg tgagcagagc cttcgagtta gcaacaccgc agacacgtaa gtcgatatgt
    20101 ttattcttca accagctttg ctgacgcttc agttgctcca gcagcgcacc gccaacgcca
    20161 ccgacgccaa tcacaaacac ttcgataacc tgatcggtat tgaacagcat ctgatgagta
    20221 acgcgcacgc cagtggtcgc atcatcgtta tttaccacga cagagattga gcgttcagaa
    20281 gatccctgag caatagcgac aatgttgata ttggcgcggg ccagcgcggc aaagaatttc
    20341 gccgagatcc cacgcaaggt gcgcatacca tcacctacca ccgagataat ggccagccgt
    20401 tccgtcaccg ccagcggctc cagtaagcct tctttcagtt ccaggtagaa ctcttcctgc
    20461 attgcccgtt cagctcgcac acagtcgctt tgcggaacgc agaaactgat actgtattcg
    20521 gaagatgatt gcgtaatcag caccacggaa atacgggcgc gtgacattgc agcaaagacg
    20581 cgcgccgcca tgccgaccat tcctttcatc cccgggccgg aaacgctgaa cattgccata
    20641 ttattcagat tggaaatgcc cttgaccggt aattcgtctt catcacgact ggcaccaatg
    20701 agcgtacctg gagcttgagg atttccggta tttttaatca ggcaagggat ctggaactgg
    20761 gcgatggggg taatggtgcg ggggtgaaga actttagcgc cgaagtagga aagctccatc
    20821 gcttcctggt aggacatcga tttcaacaac ctcgcatcgg gcacctgacg cgggtcgcag
    20881 gtatataccc cgtcaacgtc cgtccaaatc tcgcaacaat cggcgcgtaa acaggcagcc
    20941 agcaccgccg cggagtagtc ggaaccgttg cgtccaagta ccaccagttc gcctttttca
    21001 ttaccggcgg tgaaacctgc catcagcacc atgtgatcag ccggaatacg acttgccgca
    21061 atacggcggg tggactctgc aatatcgaca gtagattcga ggtaatgccc cactgccagc
    21121 agtttttcga ccggatcgat aacggtaacg ttgtgaccgc gcgcttctaa tacgccggcc
    21181 ataatggcga tcgacatttt ctcgccacgg caaatcagcg cagcgttaat gctatccggg
    21241 cactgcccta acaaactaat gccatgcagg acatgtttta tttgggcaaa ttcctggtcg
    21301 acgaaagttt tcaattgcgc cagcgggaat cccggctggg cggcggcgag tcccgtcaga
    21361 agttcggcaa aaatacgttc ggcatcgctg atattgggta aagcatcctg gccgctaatg
    21421 gttttttcaa tcatcgccac caggtggttg gtgattttgg cgggggcaga gaggacggtg
    21481 gccacctgcc cctgcctggc attgctttcc agaatatcgg caacccgcag aaaacgttct
    21541 gcatttgcca ctgatgtacc gccgaacttc aacactcgca tggttgttac ctcgttacct
    21601 ttggtcgaaa aaaaagcccg cactgtcagg tgcgggcttt tttctgtgtt tcctgtacgc
    21661 gtcagcccgc accgttacct gtggtaatgg taatggtgat ggtggtggtg atggtggtgg
    21721 taatggtggt gctaatgcgt ttcatggatg ttgtgtactc tgtaattttt atctgtctgt
    21781 gcgctatgcc tatattggtt aaagtattta gtgacctaag tcaataaaat tttaatttac
    21841 tcacggcagg taaccagttc agaagctgct gtcagagact ctttttttaa tccacacaga
    21901 gacatattgc ccgttgcagt cagaatgaaa agctgaaaaa tacttactaa ggcgtttttt
    21961 atttggtgat atttttttca atatcatgca gcaaacggtg caacattgcc gtgtctcgtt
    22021 gctctaaaag ccccaggcgt tgttgtaacc agtcgaccag ttttatgtca tctgccactg
    22081 ccagagtcgt cagcaatgcc atggctcgtt cgcgtaaagc ttgcagttga tgttggtctg
    22141 ccgttgtgtc acttttcgtc ggttgttgta ttaatattgc taattgatag caatagacca
    22201 tcaccgcctg ccccagattg agcgaaggat aatccgccac catcggcaca ccagtaagaa
    22261 cgtcagccaa cgctaactct tcgttagtca gcccggaatc ttcgcgacca aacaccagcg
    22321 cggcatggct catccatgaa gatttttcct ctaacagcgg caccagttct actggcgtag
    22381 cgtagtaatg atatttcgcc cgactgcgcg cagtggtggc gacagtgaaa tcgacatcgt
    22441 gtaacgattc agccagtgtc gggaaaactt taatattatc aataatatca ccagatccat
    22501 gtgcgaccca gcgggtggct ggctccaggt gtgcctgact atcgacaatc cgcagttcgc
    22561 taaaccccat cgttttcatt gcccgcgccg ctgccccaat attttctgct ctggcgggtg
    22621 cgaccagaat aatcgttata cgcatattgc cactcttctt gaccaaatga ccgcgaacag
    22681 ggtgatcact gtcaacttat tacgcggtgc gaatttacaa attcttaacg taagtcgcag
    22741 aaaaagccct ttacttagct taaaaaaggc taaactattt cctgactgta ctaacggttt
    22801 aattgttaaa aaatgctaca tatccttctg tttacttagg ataattttat aaaaaataaa
    22861 tctcgacaat tggattcacc acgtttatta gttgtatgat gcaactagtt ggattattaa
    22921 aataatgtga cgaaagctag catttagata cgatgatttc atcaaactgt taacgtgcta
    22981 caattgaact tgatatatgt caacgaagcg tagttttatt gggtgtccgg cccctcttag
    23041 cctgttatgt tgctgttaaa atggttagga tgacagccgt ttttgacact gtcgggtcct
    23101 gagggaaagt acccacgacc aagctaatga tgttgttgac gttgatggaa agtgcatcaa
    23161 gaacgcaatt acgtacttta gtcatgttac gccgatcatg ttaatttgca gcatgcatca
    23221 ggcaggtcag ggacttttgt acttcctgtt tcgatttagt tggcaattta ggtagcaaac
    23281 atgcagaccc cgcacattct tatcgttgaa gacgagttgg taacacgcaa cacgttgaaa
    23341 agtattttcg aagcggaagg ctatgatgtt ttcgaagcga cagatggcgc ggaaatgcat
    23401 cagatcctct ctgaatatga catcaacctg gtgatcatgg atatcaatct gccgggtaag
    23461 aacggtcttc tgttagcgcg tgaactgcgc gagcaggcga atgttgcgtt gatgttcctg
    23521 actggccgtg acaacgaagt cgataaaatt ctcggcctcg aaatcggtgc agatgactac
    23581 atcaccaaac cgttcaaccc gcgtgaactg acgattcgtg cacgcaacct gctgtcccgt
    23641 accatgaatc tgggtactgt cagcgaagaa cgtcgtagcg ttgaaagcta caagttcaat
    23701 ggttgggaac tggacatcaa cagccgttct ttgatcggcc ctgatggcga gcagtacaag
    23761 ctgccgcgca gtgagttccg cgccatgctt cacttctgtg aaaacccagg caaaattcag
    23821 tcccgtgctg aactgctgaa gaaaatgacc ggccgtgagc tgaaaccgca cgaccgtact
    23881 gtagacgtga cgatccgccg tattcgtaaa catttcgaat ctacgccgga tacgccggaa
    23941 atcatcgcca ccattcacgg tgaaggttat cgcttctgcg gtgatctgga agattaatcg
    24001 gctttaccac cgtcaaaaaa aacggcgctt tttagcgccg tttttatttt tcaaccttat
    24061 ttccagatac gtaattcatc gtccgttgta acttctttac tggctttcat tttcggcagt
    24121 gaaaacgcat accagtcgat attacgagtc acaaacatca tgccggccag cgccaccacc
    24181 agcacactgg ttcccaacaa cagcgcgcta tcggcagagt tgaggagtcc ccacatcaca
    24241 ccatccagca acaacagcgc gagggtaaac aacatgctgt tgcgccaacc tttcaatacc
    24301 gcctgcaaat aaataccgtt cattaacgcc ccaatcagac tggcgattat ccatgccacg
    24361 gtaaaaccgg tatgttcaga aagcgccagc aagagcaaat aaaacatcac caatgaaagc
    24421 cccaccagca aatattgcat tgggtgtaaa cgttgcgcgg tgagcgtttc aaaaacaaag
    24481 aacgccataa aagtcagcgc aatcagcaga atggcatact tagtcgcccg gtcagttaat
    24541 tggtattgat cggcgggcgt cgtcactgcg acgctaaacg cggggaagtt ttcccagccg
    24601 gtatcattgc ctgaagcaaa acgctcaccg agattattag caaaccagct gctttgccat
    24661 tgcgcctgaa aacctgactc actaacttcc cgtttggctg gtagaaaatc acctaaaaaa
    24721 ctgggatgcg gccagttgct ggttaaggtc atttcgctat tacgccccgc aggcaccaca
    24781 gaaagatcgc cggtaccgct taaattcagt gccatattca gcttcaggtt ctgcttccgc
    24841 cagtcccctt cgggtaaagg gatatgcacg ccctgcccgc cttgctctaa cccgatgccg
    24901 ggttcaatag tcagcgccgt tccgttaact tcaggcgctt tcaccacacc aataccacgc
    24961 gcatccccga cgctaatcac aataaatggc ttgccaaagg tgatatttgg cgcgtcgagt
    25021 tcgctaagac gcgaaacatc gaaatcggct tttagcgtta aatcactgtg ccagacctga
    25081 ccggtataaa tccctatctt gcgttcttcc acgttctgat tgccatcaac catcaatgac
    25141 tcaggtaacc aaaaatggat aaaacttcgt ttccgctcca cggttttatc ctcttcctgc
    25201 accgtataaa gctcggtcac aggaatagcg ataagaggcc caacgagttt ttgcggcccg
    25261 ctggtacttt ggcgaatcgc atcttccaca tcgctacggt aatcggcacg ttcgacaatc
    25321 acctgccgaa tcagcattat cggaatcaac aacagcaata ctgcaccaaa cagggtggtc
    25381 attttccaga acagggggga tttcaacata gtcttctcct ttgcaatggc agcaggatac
    25441 gaaggctatg tgggaagaat ttgaagctat gtgaagtgac ggtgaagccg aagcgaggcc
    25501 agcacgccac cttcctgcac gttgcgcagc gtgacttcgc cgttaaacaa acgggcgacc
    25561 tcactgacaa acgccaaccc cagaccgctg cttttttgcc catttgcacg cggcaaagag
    25621 taaaagcgtt caaaaatacg tgacagcgcg tagtcaggaa taccactacc ggtatccagc
    25681 accttaaggg cgacgtgttc ctgatccact tcggcgctta gcgttatgcg accgctcttg
    25741 ggggtaaaat cgatggcgtt atccagtaaa ttccccagcg cctgctccag taacgccggt
    25801 tcagcagcaa cgttaacctc agtaggcata acatgcaaag tgatgttttt ttctgccaac
    25861 tgcacggtgc gcgcttcgct gacgcggcga aataatgccg ccacatcaac agcagtcaga
    25921 acgacttcct gacgattctc cagtcttgcc tggcgtagta acgtttctac caatgcctgc
    25981 atacgcgcat tttgcgtcag aatgttgtcg gtaaaacgag ccaccacttc cggtggcgga
    26041 ccttcgcgta aaatttccgc cgcgccacga atcgccgcca gtgggctttt tagctcatgg
    26101 gttaacgcat aaacatactg ctcaatatag tttttccctt ccagcttcac gcgcatactt
    26161 tccagcgcct gcgcaagctt acgcaactcg ctactgccga gttcaggaag aggaacgggc
    26221 ttattgtcag tgacggaatc ggcatagcga gtgagccggg caatggagcg gttgatccac
    26281 caaaccatgc ctgcgccaat caccagtgca atccccagca aaatggcgct ggcccataaa
    26341 attcgccgct cgctgcgctt aatgaccgga gccatcgccg cgttcggttt gcctacgctc
    26401 aaaacgccaa taagccgcga gccgtccata atcggcgcgg caacatacat caccgaactt
    26461 tcgggatcgg caggattttg caacgtgctg cgcgcaccat actgaccacg caacgttagc
    26521 cagacgtcat tccagcgcga ataatcctgc ccaacggctt tatttgccga atcgaacaat
    26581 actttgccct gcgcatcggt catatagaca tgatattcat tgcgcacttt gttaatgcca
    26641 ccgatattag cgcgaaacgg gcgatgttgt agctgattaa acgcctgcgc cagttgccca
    26701 tgcgttgggt ccccagagag caaatcggga cgcgccagct ccgccagcaa cgttgcggtg
    26761 tcgatcagcg tcccctccgt tgctcttcgc acgcccggtt taacttcttt gacaaaaata
    26821 gccagtacga accaggccgc caccgccacc agtaaaaaat agcccagcaa caaccgcatg
    26881 ccgatacgca ttacaggctc ctcaggctgt atcccatgcc gcgatgagta ttaatcggtg
    26941 aaagatcggg gttgatggcg cgcagcttgg cacgcagcgt tttaatgtgg gtatcgacgg
    27001 tgcgatcgta ggtgtcctgc gcatcttccc acacgatatc catcagttgc tggcgggacc
    27061 agacgcggcc tggcgacttg agtaacgtct tcagcaataa aaactcatac cgcgtcagcg
    27121 ttaatggcgt gtcaaaccag ctgatctgcg ccgcgggttc attcaattca aaatgaccaa
    27181 tacggatgac gggagacggc gtcgagaact tcttcacccg acgcagcaag gtgcgcaccc
    27241 tagcgcacac ttcgcggggt gaaaacggtt tagccacata gtcgtcagcg ccaatttcca
    27301 gcccaagcag gcgatcgacc tcttcacttc gggccgtcag gaacagtaca ggtaacgccg
    27361 gatggagcgc cagtaactgg cggcacaatt caaagccact gatatcaggc aaaccaacgt
    27421 cgagaatcat gacatcggga acctgctggc gagctttatc cagcaccggc aagcctcgct
    27481 caaagacctc gacggcaaaa ccttcctgct gcaacatgta gaccagcgtg tcggctatcc
    27541 cttgctcatc ttccactaac cagaccgttt cccgttgcat aaatcgcctc tgttattgtc
    27601 gccacggcat gacaggaacc gcgctaatcg cgtttttcgg cgaaccttct acaactttgt
    27661 cggagtaagc cagataagcg agcgcgttgc gtttggcgtc ataaaagcgc acgacctgta
    27721 acgacttaaa gaccagggac gtgcgttttt tgaatactac ctcgccctga gctttgccgt
    27781 ttttaatacg atccgacagt tcaatcggcc cgacttgctg acaagaaata gccgcatcgg
    27841 aggtatcttc cgccagaccc aatccccctt taataccacc tgttttcgcc cggctcacat
    27901 aacaggtgac atttttcaca tcgggatcgt caaaggcttc cacaacaatt ttgtgatccg
    27961 ggccgatcat tttaaatacg gtgtcgaccg aaccaatctc ttctgcatga gccaatggcc
    28021 ccagcattat caggcttaaa gacaggatca aatgcttgta tttcatattg ttaccatttt
    28081 tatctattac acgagcaatt agttcgtcac ggtaaataac atattgatta agaacaaaat
    28141 ctcaatactt ttatttccgt caagacctaa aacatactct actaagaaaa aaacactgaa
    28201 tgctaaaaca gcaaaaaatg ctattatcca attacctgat gtcaggtgct cgttgttgaa
    28261 aggatgagga tattttatgg atcaggccgg cattattcgc gaccttttaa tctggctgga
    28321 aggtcatctg gatcagcccc tgtcgctcga caatgtagcg gcgaaagcag gttattccaa
    28381 gtggcactta cagagaatgt ttaaagatgt cactggccat gctattggcg cgtatattcg
    28441 tgctcgtcgt ttgtcgaaat cggcggtcgc actacgcctg actgcgcgtc cgattctgga
    28501 catcgcgctg caataccgct tcgactctca acagacattt acccgcgcat tcaagaagca
    28561 gtttgcccag actcctgcac tttaccgccg ttctcctgaa tggagcgcct ttggtattcg
    28621 cccgccgctg cgtctgggtg aattcactat gccagagcat aaatttgtca ccctggaaga
    28681 tacgccgctg attggtgtta cccagagcta ctcctgttcg ctggagcaaa tctctgattt
    28741 ccgccatgaa atgcgttatc agttctggca cgattttctc ggcaacgcgc cgaccattcc
    28801 gccagtgctc tacggcctga atgaaacgcg tccgagtcag gataaagacg acgaacaaga
    28861 ggtattctat accaccgcgt tagcccagga tcaggcagat ggctatgtac tgacggggca
    28921 tccggtgatg ctgcagggcg gcgaatatgt gatgtttacc tatgaaggtc tgggaaccgg
    28981 cgtgcaggag tttatcctga cggtatacgg aacgtgcatg ccaatgctca acctgacgcg
    29041 ccgtaaaggt caggatattg agcgatacta cccggcagaa gacgccaaag cgggagatcg
    29101 cccaattaat ctgcgctgtg aactgctgat tcctatccgc cgttaacgct gcagctcatc
    29161 taatgcaggg gcgtccagat gcgagatgtc ccctgcggtt tcaacgaccc agccagacgc
    29221 cagccacagg ctttcctgat aatccacgcg cgaaatagaa cagttacgca gacgtaagcg
    29281 gcgctctgcc catgctggta atccgagaat cgtactcacc agacatccca gtgcaatacc
    29341 atggctcacc agcagcggtc ggcttccctg cggtaagtcc cggcaagatt ccagtgccgc
    29401 attgacgcga tcgctgagct cctgcattga ctcgccttca ggaatacgcc catcaacggt
    29461 gccattgacc agctgccgac gccagttctc ttcttcttcg gtcagagaat cgatatgtct
    29521 tttttccagc acacccatgt ttaattcacg caggcgagaa tcaaagatga tgtcacagcc
    29581 gcaggcctgg gcgatgattt ccgccgtacg ccgggtgcgt cctaaatcgc tactgatgat
    29641 atgcgtaatg ccaagctctt tggcacgcgt cgccacctgc atcgcctgtt gctcaccttt
    29701 ggcggtcagc gggctgtcag actggccctg aatacgtcgc tcggcgttcc actgcgtttc
    29761 accgtggcgg actaggtata cctgtaacat gctttttttc cgtatactgc tgtaacgtta
    29821 attttctgag taatgctgat tatgcaccaa gttgtctgtg cgaccaccaa tcccgctaaa
    29881 attcaggcca ttctgcaggc atttcacgag atcttcggcg aaggatcctg ccatattgca
    29941 tccattgccg tcgagagcgg tgtaccggaa cagccctttg gcagtgagga aacgcgcgct
    30001 ggcgcacgaa accgggtagc caatgcccgc cgtttacttc cagaggctga tttttgggtg
    30061 gcgattgaag ctggcattga tggcgacagc actttcagtt gggtggtgat tgaaaacacc
    30121 acccagcgcg gcgaggcgcg ttcggcaact ttgccgttgc ctgcggtgat attggaaaaa
    30181 gtacgtgaag gtgaggcgct tggtcccgtg atgtcgcgtt ataccggtat tgatgagatt
    30241 ggtcgtaagg aaggggcgat cggcgtgttt accgccggga aactcactcg cgccagcgtc
    30301 tatcaccagg cggtgatcct ggcgctgagt ccgtttcata atgcggtgta ttaagcgcct
    30361 gatgcgacgc tgccgcgtct tatcatgcct accaaacata ttgaaattac gggtatttgt
    30421 aggacggata aggcgttcac gccgcatccg gcattcggtg cacgatgcct gatgcgccac
    30481 gtcttatcag gcctacaaaa tcaatcgctt ttcagcaaca cctcttccag ccactggcgc
    30541 agctccacgg gcgcggcttt caggctgtta gatccacgcg taatcgtcgc gatgcctgcg
    30601 ccgagttcat tttttaactc acgctggctc atttcgccgc gcaacagctc ttcgacaata
    30661 cgcacgcgag tccccaacgc ttcgcgctca tctggcgtca gcatcaggtt taacaacggt
    30721 aaatggagat cgttttggta ggcattctta agcaggtcga caaaacgtaa ccactcctgg
    30781 tgacgctgtt ctgccatcgc tgctgaatag ggtgattgtt gggccataat atgtcgccat
    30841 tgttagcggg ggaagcaaaa tgcctccccc ggttgtactc gctaaagagt acgatagcat
    30901 atcataaacg tgcggatcag taacgacgtc cccattccgt ggcgctcatc aacgtcggtt
    30961 tatcccccat gaaatagcgg tagtaagcgt cataagccag cacgttcttc acataaccgc
    31021 gcgtctcgga gaacggaata ctctcgacaa atgccacagc gtcgatacgc ccggcgctgt
    31081 tgccaagcca ggttcgcacc cgtccaggtc cggcgttata agctgctgag gagaaaatac
    31141 ggttattacc aaattgctga taaacatatt gcaggtaact ggtgccaatg ttgatattcg
    31201 tttccggatc cagcaattgc ccagggctgc tataaccagg aatagagaac atcttcaccg
    31261 tatgggtcgc cgtgcccggc ataatctgca tcaggccgct ggcccctacc ggtgatttca
    31321 ctttcggatt ccaggcgctc tcctgacggg caatcgccat cgcatagctt tgcgggatct
    31381 ccttcccact ggtgtaacgt ttgaaaagat cgttgtaagc cagcgggaat cgctcttcca
    31441 gatgatccca aagcttcccg gcgatcgttg cctgaacgct aagatcccac cattggttgt
    31501 tgaaagcata ccgcgccagt tgagcctgct ctgtttttga cttgctcttc accagattgg
    31561 cccactcgct acgcgcggtg ttatcgagat tccagtacat caactcgcgc acgcgcgcca
    31621 tctccggccc ctgagtcagg gcgctgtcaa cattctgcgg cgctttatca atcttcagct
    31681 catactcttc gccgatgcgt tgtgcagcaa ccatcgggta gaaaccacgc tgttgcatga
    31741 gttgatgcaa aatctctttt gcttcagctt cacgtccgcg ttccagcaat aaatccgcct
    31801 gccagtaacg ccattcatct ttctctttcg cttccatagg cagacgcgcc agccaggtat
    31861 tcaggccgcg acgatcgcca gtgccaagcg ccattcgcac acggcgttca ataagcgaag
    31921 tagattgcga gcgcataatg gcgtcatcgc gccatttcgc ctgctcgtcg gtgacatcgt
    31981 tgcccatcaa acgccaggcg acgatatcgc gcagctcctg aatctgatct tcattaagct
    32041 gctgcgcctg ggcaagcgat gggatcatca gccgtgcatt ttcagcatcc tgccgcgcca
    32101 cactggcaaa cgccaccgcc gccatttgac gggtaaaatc ggtcgctccg gttgtacgcg
    32161 cgaaggtcag taccgtattg gggttgttcg ccagtgaaat gattgccgag gcgatagtct
    32221 ggtaatcggc aggcatctgc cctgccagca ctgttaccag gccagtgtta cccgctttca
    32281 tcgccagacg gatacgctct aaatacgcca gcggatcttg tttacctgac gcacgccaga
    32341 cgctaaataa cttgtcacag gcgttaggct ggctcttgcc ggttagccac agctctttcg
    32401 ccccttgcca ggcttcttca ctctgcccgg tgttccattt cgcatagtag taattacatt
    32461 gtgcttcggt agttccgggc ttttccgggc taaaggctaa caggccacgc cagtcttcac
    32521 gccgcgccag ttcattgacg aaacgagatt gcagcgtgcg agcgggagga agcgtggggt
    32581 tagcgcgaac aaagttagtg accgtcaccg ccggttgatt catcaggtca tcggtgatct
    32641 ggcggtattc caggtaggga taaagcggat aatccttcag tccaggcatc atttgttcga
    32701 ccacatccat ttgtcgatta tcccaggcct gcttgatttg cgcgtaacga ctacgctgct
    32761 catccagtga gtcggctcgc gccacgctgc tgaccgtcag cagacagaca ccggcagcca
    32821 acagccgcca ggtaacttgt ttggcttttt ccacaagcgc atcctctaag tgtaaataca
    32881 tcaatgcagc gtcatgccgc gtaatagatt tatgctaacc agtcattgcc gtttacgcca
    32941 cgttacggac acttttttac ttttactgcg agggtgatcg accggctggg attaggcggg
    33001 gaaaaaggct acacttcgcc tttgaaaact catcatcacg ataaacatag aggcgaagtc
    33061 caacgtggct caattcgttt ataccatgca tcgtgtcggc aaagttgttc cgccgaaacg
    33121 tcatattttg aaaaacatct ctctgagttt cttccctggg gcaaaaattg gtgtcctggg
    33181 tctgaacggc gcgggtaagt ccaccctgct gcgcattatg gcgggcattg ataaagacat
    33241 cgaaggtgaa gcgcgtccgc agccagacat caagattggt tatctgccgc aggaaccgca
    33301 gctgaacccg gaacacaccg tacgtgagtc cattgaagaa gcggtttcag aagtggttaa
    33361 cgccctgaaa cgcctggatg aagtgtatgc gctgtacgcc gatccggatg ccgattttga
    33421 caagctggcc gctgaacaag gccgtctgga agagatcatt caggctcacg acggtcataa
    33481 tctgaacgta cagctggagc gtgcggcgga tgcgctacgt ctgccggact gggacgcgaa
    33541 aatcgctaac ctctccggtg gtgagcgtcg tcgcgtggcg ttgtgccgcc tgctgctgga
    33601 aaaaccagac atgctgctgc tcgacgaacc gaccaaccac ctggatgccg aatccgtggc
    33661 ctggctggaa cgcttcctgc acgacttcga aggcaccgtg gtggcgatta cccacgaccg
    33721 ttacttcctc gataacgttg cgggctggat cctcgaactt gaccgcggtg aaggtattcc
    33781 gtgggaaggt aactactcct cctggctgga gcagaaagat cagcgtctgg cgcaggaagc
    33841 ttcacaagaa gcggcgcgtc gtaagtcgat cgagaaagag ctggagtggg tacgtcaggg
    33901 aactaaaggc cgccagtcga aaggtaaagc acgtctggca cgctttgaag aactgaacag
    33961 caccgaatat cagaaacgta acgaaaccaa cgaactgttt attccacctg gaccgcgtct
    34021 gggcgataaa gtgctggaag tcagcaacct gcgtaaatcc tatggcgatc gtctgctgat
    34081 tgatgacctg agcttctcga tcccgaaagg agcgatcgtc gggatcatcg gtccgaacgg
    34141 tgcgggtaaa tcgaccctgt tccgtatgat ctctggtcag gaacagccgg acagcggcac
    34201 catcactttg ggtgaaacgg tgaaactggc gtcggttgat cagttccgtg actcaatgga
    34261 taacagcaaa accgtttggg aagaagtttc cggcgggctg gatatcatga agatcggcaa
    34321 caccgagatg ccaagccgcg cctacgttgg ccgctttaac tttaaagggg ttgatcaggg
    34381 taaacgcgtt ggtgaactct ccggtggtga gcgcggtcgt ctgcatctgg cgaagctgct
    34441 gcaggttggc ggcaacatgc tgctgctcga cgaaccaacc aacgacctgg atatcgaaac
    34501 cctgcgcgcg ctggaaaacg ccctgctgga gttcccgggc tgtgcgatgg ttatctcgca
    34561 cgaccgttgg ttcctcgacc gtatcgccac gcacattctg gattaccagg atgaaggtaa
    34621 agttgagttc ttcgaaggta actttaccga gtacgaagag tacaagaaac gcacgctggg
    34681 cgcagacgcg ctggagccga agcgtatcaa gtacaagcgt attgcgaagt aatgcgtaaa
    34741 atgccggatg cggcgcgaac gccttatccg gcctacaaaa ccagcaattt caatacgttg
    34801 taaaactgta gacctgataa gacgcggtaa gcgtcgcatc aggcattgag caccgaatgc
    34861 cggatgcggc gtgaacacct tttccggtct acaacagcct tagcatcctc aatttcgtgg
    34921 cgggccaccc gtgcaggttt tttcaaacct tgtttaatct cgacagcctc ttccagagag
    34981 gttttcaact catcaaaaaa actcatttta tacctcgcct ttgagcagtt tcatcacggt
    35041 tatctctgct cccccatcat ctcccgcacc agctccacgc agcgcaggaa acggctgtcg
    35101 taatcttcct cttcaacccg cacaaattcg atattgttct cttccagcat ctccaccagc
    35161 agattctgga actctttacg atccaccgaa ctgccgaggc tgcgtaaacc atccaccacc
    35221 cacggcgtgt tgttctccag caggatcacc agatcgaaac ggtattcatc aatcagcgcc
    35281 tgcacgaacg gatgttcccg cccttcgtac tttttgcaga acgcctgagt ggtgacaaaa
    35341 tcggtatcga taaatgccac tttattggca tatttcaccg caaaatcaat gtattgagcg
    35401 tggcccagcg cgattttatc gtagtcagaa tactgcaatg cgatctcatc accgccgagg
    35461 tgtgaaaaga cataatcgcg gccatattcc cacgcactgg tggtgttgaa gatattggca
    35521 agtttgttta ccagggtgga tttaccgctc gactcgccgc caaggatcgc cacggtacgc
    35581 acaaaaaacg gcttcacttc ggtaggaata tattcccagt agcggaacgg gttttcgcgg
    35641 atctgcgcac cgctgatact cataaaggta cgtttcggat cgaccagcac cgtctcgatc
    35701 cccagatgtt ccatatactg tggcgcatcg gcttcttccg aggtgtagat cagatccggc
    35761 tggatcccct tttcagccat aaactttttg ataccgttgc tccacacatc ccagccgtgc
    35821 ggatacggct ccatgccctc ttcgttgaaa gcatgaatgc gaatattttt ctgatattta
    35881 aaagtttgca ataaccagcg cagacgatcc ggcacggtcg gctgctgcga catggcactg
    35941 tcttcgaaca acgcgcggtc acgggtatcg tcaaaaccca taatgatatg cagctcgtca
    36001 acctggctac aggcgcgctg gataaggtag atatgtccgg tatgcagtgg gtagaactta
    36061 ccgaatacga caccgatcgt tttcttctgc cggggaaact caagccccaa aaaacggtgc
    36121 aacgcctcca gcttttgcgc gctggggctt ttgattttgg cattcagtaa ctggcttaaa
    36181 taccctttgg tcataccgct ggcatcagct acctgctgta gcgtgcagcc ctgttgcttg
    36241 atggcagttt tcaggtaatc aaatgacgac atatctccct ccgtatttct cattataagt
    36301 cgtcgaacac gctaagcgcg tcggagagtt ttttaacgcc aaaaatctgc atcccttccg
    36361 gcgctttttt cggtacgtta gccgccggaa caatcgcccg gcgaaaaccg tgtttcgccg
    36421 cttcagagat tcgttcctga ccgctgggca ccgggcggat ctcccctgcc agcccgactt
    36481 caccaaacac caccagatcc tgcggtagcg gtctgtcacg caggctggaa accatcgcca
    36541 gcagtaacgc taagtcggcg ctggtttcgg ttacctttac gccgccgacc acgttcacaa
    36601 acacatcctg atcggccatt tgcagaccac cgtgacggtg caagacagcc agcaggattg
    36661 ccagacggtt ttgttccagc cccactgcca cgcggcgcgg attcgccatc atcgagtgat
    36721 cgaccagcgc ctgaatctcc accagcaacg gacgcgttcc ttcccacacc accatcacgg
    36781 agctgccgga ggtcacttca tctccgcgac ttaagaaaat tgccgaaggg ttgctgactt
    36841 cacgcagccc ctgttcggtc atcgcgaaga cgcccagctc attcaccgcg ccgaagcggt
    36901 ttttatggct gcgcaaggtg cgaaaacggg agtcggcatc gccatctaaa agcaccgaac
    36961 agtcgataca gtgttccagc actttcgggc cagccagcga accatctttg gttacgtgcc
    37021 ccaccatgac aatcgccaca ccgcgcgttt tggcgaagcg tgtcaaataa gccgccgttt
    37081 cacgcacctg cgccacgcta ccaggcgatg actgtacatc cgccatatgc atcacctgga
    37141 tcgagtcaat taccatcagc ttcggttgct cttcttcggc aatcaggcag atctgttcga
    37201 tgctggtttc cgacaacata ttgagattgt cagtcggcag gccaaggcga tgagcgcgca
    37261 ttgccacctg ttgcagcgac tcttcgccgg tgacatacag cgttttcatc tgctgggcca
    37321 gtttgcacag cgtttgcagt agcagcgtgg atttccccgc accagggtta ccgccaatca
    37381 gaatggcact tcctggcacc acgccgccgc ctagtacgcg gtcgaactct ttaaatccgg
    37441 tggaaaaacg cggcagctct tcaaggctga tatcggagag tttctggact tttgccaccc
    37501 cggcgctacc ggcatagccg ctgagacgct cgttacgcgc caccgtaggc gacgcagcaa
    37561 gacgcacctc ggtgatggtg ttccaggcat gacaggcact gcactgcccc tgccagcgcg
    37621 gataatcggc cccgcattca ttacaaacaa aggcgcgttt tggagctttt gccacggtgt
    37681 acctcgttaa tgctgtgccg cccgcaggat ggcgggcgag caattacttc tgattcaggc
    37741 tgcctgagag gatgcagaat acccccatca ggtcagcgtg acggatggtg acttccgcct
    37801 tttcattcac ttttggcttg gcatggtagg caatccccag ccctgccgct ttgatcatcg
    37861 gcaggtcatt ggctccatcg ccaatcgcca cggtctgcgc cagcgggatt tcatactcct
    37921 gcgcgaggcg agtcagagtt ttcgctttgt actgcgcgtc tacgatgtcg ccgatcacat
    37981 tgccggtaaa tttaccgtcc atgatctcca gttcattggc taccacggct gtcaggcgca
    38041 gcttgtcgcg caggtattca gcaaagaaag taaagccgcc ggaggcaatc gccactttcc
    38101 agcccagcgt ttccagcttg agcaccagtt gcgttaagcc tggcatcagc ggcagatttt
    38161 cacgcacctg ttgcagaata ttggcgtcag cgcctttcag cgtcgccacg cggctgcgca
    38221 ggctggcggt aaaatcgagt tcgccgcgca tcgcccgttc ggttacttcc gccaccatct
    38281 cgcccgttcc ggccagtttg gcaatttcat caatacattc aatctggatg gcggtggaat
    38341 ccatgtccat caccagcaaa cccggcgtgc gcaggtgcgg gatttttccc agcggcgcga
    38401 catccagctg cgcttcgtgg gccaggcgtg tagcccgtgc ggtgagtgaa cctgccagac
    38461 gaatcacctg ataatcttcc acgcaccagg cggcaacaat caccatcgcc gcacccagtt
    38521 tgctctggta ttgggtcaga cgctgtttat ccagcccacg accatacagc agccagccgc
    38581 tacgacctgc gtggtaatcc agtggcatca cttcatcacc acttaatgaa agaggcagac
    38641 ccggccataa agagacatct tcaggcaggt cgcaccaggt aatgttaggc attaaggctc
    38701 ctgtaaaatc gttcgaagca gggaaaataa cgcatgaggc taccttgtat ccattgcttc
    38761 tggcaacatt aagtctcaaa ttttcaaagg gtggaagatg gctcgcacaa aactgaaatt
    38821 ccggctgcat cgggcagtga ttgtcctgtt ctgtcttgcc ttgttagtgg cactgatgca
    38881 gggggcgtca tggtttagtc aaaaccacca gcgacagcgt aatccacagc tggaagaact
    38941 ggcccgcacc ctggcgcgtc aggtgacgct gaacgttgca ccgctgatgc gtaccgactc
    39001 accggatgaa aaacgcattc aggcgatcct cgatcagtta acggatgaaa gccgtatcct
    39061 cgacgcgggt gtgtatgacg aacaaggcga tcttatcgca cgttctggcg aaagcgtcga
    39121 agtgcgcgat cggctggcgc tcgacggtaa aaaagcaggc ggctatttta accagcagat
    39181 tgtcgagcca attgccggga aaaacggacc gctcggctat ctgcgcctga cactcgacac
    39241 ccatacgctc gccaccgaag cccaacaggt ggataacacc actaacattt tacgcctgat
    39301 gttgctgctc tcactggcaa tcggcgtagt gctgacccgc acgctgctac agggtaaacg
    39361 cacccgctgg cagcaatcgc ccttcctgtt aaccgccagc aaaccggtgc cggaagagga
    39421 agaaagcgag aaaaaagagt gccccattac tacaagaaag gaaatcgtta tgtccacatt
    39481 acgcctgctc atctctgact cttacgaccc gtggtttaac ctggcggtgg aagagtgtat
    39541 ttttcgccaa atgcccgcca cgcagcgcgt tctgtttctc tggcgcaatg ccgacacggt
    39601 agtaattggt cgcgcgcaga acccgtggaa agagtgtaat acccggcgga tggaagaaga
    39661 taacgtccgc ctggcgcggc gcagtagcgg tggcggcgcg gtgttccacg atctcggcaa
    39721 tacctgcttt acctttatgg ctggcaagcc ggagtacgat aaaaccatct ccacgtcgat
    39781 tgtgctcaat gcactgaatg cgctcggcgt cagtgccgaa gcgtccggac gtaacgatct
    39841 ggtggtgaaa accgccgaag gcgaccgcaa agtctcagga tcggcctatc gcgaaaccaa
    39901 agatcgtggc ttccaccacg gcaccttgct gctcaatgcc gaccttagcc gcctggcaaa
    39961 ctatctcaat ccggataaaa agaaactggc ggcgaaaggc attacttcag tgcgttcccg
    40021 cgtgaccaac ctcaccgagc tgctgccggg gatcccccat gagcaggttt gcgaggccat
    40081 aaccgaggcc tttttcgccc attatggcga gcgcgtggaa gcggaaatca tctccccgga
    40141 caaaacgcca gacttgccaa acttcgccga aacctttgcc cgccagagta gctgggagtg
    40201 gaacttcggt caggctccgg cattctcgca tctgctggat gaacgcttta gctggggcgg
    40261 cgtggaactg catttcgacg ttgaaaaagg ccatatcacc cgcgcccagg tgtttaccga
    40321 cagcctcaac cccgcgccgc tggaagccct cgccgggcga ctgcaaggct gcctgtaccg
    40381 cgcggatatg ctgcaacaag agtgcgaagc gctgttggtt gacttcccgg accaggaaaa
    40441 agagctacgg gagttgtcga cgtggatagc gggggcggta aggtaactcg ctgattaaaa
    40501 tatccaagga aggaaatatg atcgatctgg aaaaccagga acgggagatt atcaatttaa
    40561 tgttcagcca gcgtatttcc tggctagccg ccgtgcgtat caggcataaa ttatcgcttg
    40621 ctgaagtaag taaaatgtta ggtatctcga ttaattctct aaaacagatc gaaaagacgg
    40681 aacggcttag ctcaaacatc aaaagcaaaa tggcagaaat ttatggttgt ccaccagagt
    40741 tgctgatatg cccttcctgg atgacggctg aacataaatg aatagattcg tgaatagata
    40801 cttgaatgga ttttattttc tatccatatg aaaaataata gtaaatctat aaatcagttg
    40861 ctattgatga atagatacta aaaagccgga gcagtccccc actccggctt aacacgacaa
    40921 tcgcctttca gcgaaacaca attactcttt atcgcccagc agaacggatt ccagtgcgat
    40981 tttgatcatg tcgttgaagg tggtctgacg ctcagcggca gtggtttgct cgtgagtgcg
    41041 gatgtggtca gacacggtgc agatggtcag ggctttcgcg ccaaactctg cagccacgcc
    41101 gtagatacca gccgcttcca tttccacgcc gagaatgccg tatttttcca tcacgtcgaa
    41161 catttcgccg tccggagagt agaacaggtc agcggagaac aggttaccaa cgcgagcatc
    41221 aatgcccagt gctttagctg catctactgc gttacgcacc atgtcgaagt cagcgatagc
    41281 ggcaaagtca tggtctttaa aacggatgcg gttaactttg gaatcggtgc aggcacccat
    41341 accgataacg acgtcgcgca gttttacgtg cggcagaact gcgccacagg aacccacgcg
    41401 gataattttc ttcacgccga aatcggtgat cagttctttg gtgtagatgg agcaggacgg
    41461 gatacccata ccgtgaccca ttacggaaat tttgcggcct ttgtaagtac cggtgaagcc
    41521 cagcataccg cgaacgttgt tcacttcacg ggcatcttca aggaaagttt cagcaatata
    41581 cttcgcacgc agcgggtcgc ctggcatcaa aactacgtca gcgaaatcgc ccatttctgc
    41641 attaatgtgt ggggtagcca ttgttttatc ctttttgtga cataacaaag gcggagtcaa
    41701 cgctccgccc aaatccatca gaacatggct ttgccatatt ccatatcaga agtaccaaaa
    41761 tattttgcca gagtctggcc gatatccgcg aaggtttcac ggtgacccag tgagcccggt
    41821 tttactttcg ggccgtaaac cagtaccgga atgtgttcac gcgtgtggtc agtaccggtc
    41881 caggtcggat cacagccgtg gtcagcggtg aggatcagga tgtcgtcatc gcgcagcaga
    41941 gacatcagct ccggcagacg gcggtcgaac agttccagac ccgcagcata accggcgacg
    42001 tcgcgacggt ggccccagga agagtcgaag tcaacgaagt tggtgaagac gatggtgtta
    42061 tcacccgctt ctttcatctc tttgatggtg gcgtcaaaca gcgcgtccag gccagtcgct
    42121 ttcacttttt tggtgatacc gcagttggcg tagatgtctg caattttacc gaccgaaacc
    42181 acctggccgt gtttttcatc aaccagtttc tgcagcacgg tcggtgctgg cggctcaaca
    42241 gccaggtcgt gacggttacc ggtacgctgg aagttaccgg ctttgtcgcc gataaacgga
    42301 cgagcgataa cacgaccgat attgtagccg ccgttggtca gctcttcacg ggcgatttcg
    42361 cacagttcgt agagtttatc cagaccgaaa gtttcttcat ggcaggcaat ctggaacacg
    42421 gagtcagcgg aggtatagaa aatcggcttg ccggttttca tgtgctcttc gcccagttga
    42481 tccagaatga ccgtaccgga agagtggcag ttaccgaggt aacccggcag attagcgcgt
    42541 tcgaccagtt tatccagcag ctcttgcggg aagctgtttt cgtgatcgga gaaatatccc
    42601 cactcaaaca gaaccgggac accggcaatt tcccagtgac cagacggggt atctttaccg
    42661 gatgacattt cgtgcgccca tgcgtacgcg ccgataactt cagcgttgcc gtccattccc
    42721 gccggaatga aaccggtaga accttcgtgt gctttcgcca gccccagacg ggtcagattt
    42781 ggcagattga gcgggccttt acgaccgtta tcagcttcgc ctttggcaca agcttctgcg
    42841 atatgaccca gggtgtcagc cccgacgtca ccaaagcgtt ctgcatcttc tgtagcgccg
    42901 atgccgaatg agtccagcac cataataaat gcacgtttca tatgttctcc gtactcagtg
    42961 cttcaaattt aaatgatcag atcagtatac cgttattcgc tgatacggcg atagacagtt
    43021 ggtgtgcttt ccggtgcttt atcggcaagt ttaattgccg ctttcaccgc tttcgccgct
    43081 tcctgccagc tgttttcgtc tttcgcgtgg ataaccgcca gcggacgctg gccgtctacc
    43141 tggtcgccca gacgtgccat atcagtaaag ccgacgctgt aatcgatggt gtcagatgcc
    43201 tgacggcgtc cgccgcccat tgcaaccact gccatcccca gcgcgcgggt atccatttca
    43261 ctgacaaaac cttcggtatc agcatagact gctttcgtca gcatcgctgt cggcagatac
    43321 ttcgcgtagt tctcaacgaa gtcggtcggg cctttttgtg ccgctaccat acgaccaaag
    43381 acttctgccg ctttaccgtt gtccagcacc gcctgcaatt tcgcgcgcgc ttcggcgtca
    43441 tctttcgcca gtttgccgga gataagcatc tccacacaca gcgccatcgt gacatcaaac
    43501 agacgcgggt tacgatattc acccgtcagg aactgcaccg cttcacgtac ttcaaccgcg
    43561 ttaccggcac tggaggccag cacctgattc atgtcggtga gcagtgcggt ggtgcgcacg
    43621 cctgcgccgt tagccacgcc aacaatcgct tcggcaaggg cttcagagag ttcgtaggtc
    43681 ggcataaacg cgccgctacc cactttcacg tccatcacca gcgcgtccag accttccgcc
    43741 agtttcttcg ccaggatcga ggcagtgatc agcgggatgg agtccacggt tgcggtaata
    43801 tcacgggtcg cgtagaaacg tttatccgcc ggagccagtg agctggtctg accgataatc
    43861 gccacgccga cgtctttaat aatttcgcgg aaacggttgt catccgggaa aatgtcgaag
    43921 ccagggatgg attccagttt gtcgagcgta ccgccagtat gaccgaggcc gcgaccagag
    43981 atcatcggaa tatagccgcc gcaggctgcg accatcggcc ccaacatcag cgaagtcaca
    44041 tcgccgacgc caccggtgga gtgtttatca acaatcgggc cattcagatg caggcttttc
    44101 cagtcgagaa cggttcctga atctcgcatc gccatggtca gcgagacacg ctcaggcatt
    44161 gtcatatcgt ggaagaaaat ggtcatcgcg agggcggcaa tctgcccttc ggagatagtg
    44221 ttgtcgcgaa taccgttgat aaagaaacga atttcttcat cgctcagcgc atgaccatca
    44281 cgttttttac gaataatttc ttgtgcgaga aacaaggtaa cctcccgagg aaaagagtat
    44341 gaggcggctt ttgccggatg cgtctacgcc ttatccggcc tacgggtaga tacgtctcgt
    44401 aggcctgata agcgcagcgc atcaggcgta aagcatctta cttagtagct gctggcgctc
    44461 ttaccgtcgc cgtgacccag cgctttcagc aggcttgcca gcaggctgga agcaccaaag
    44521 cggtagtgac gcgcatcagc ccagtcagca ccgaacagtt catctgcgat ggcgagatat
    44581 ttctgcgcat cttccgcagt acgcacgccg cccgccggtt tgaaaccaac ggttttttct
    44641 acgcccatat cacggatcac ttccatcatg atgcgcgcgc tttccggcgt cgcgttcaca
    44701 gccactttac cggtagaggt tttgatgaag tccgcacccg ctttgatgga gatttcagac
    44761 gctttacgga tcagcgcttc gtctttcagt tcgccggttt cgatgatcac tttcagcagt
    44821 acattcgctg ccgcgcaagc ctctttacag gctttcacca ggtcaaaacc aacctgctcg
    44881 ttacccgcca tcagcgcgcg gtacgggaac acaacgtcaa cttcatcagc accgtaggcg
    44941 attgccgcac gggtttctgc cagcgcgatt tcgatgtcgt cgttaccgtg tgggaagttg
    45001 gttaccgtag caatacggat ttccggggtg ccctgctctt tcagtgtttt gcgagcaatc
    45061 gggataaagc gaggatagat acagatagcg gcggtattgc cgaccggagt tttggcctga
    45121 tgacacagag caattacttt ctcgtcggtg tcgtcgtcat tcagggtggt caggtccatc
    45181 aatttcagtg cacgcaggct gcttgctttc agatcagtca tttcattctc ctggcttgtc
    45241 gccaataaaa ttcaccttgc gagtttgtta gtattctaac atctactccg caacacactt
    45301 cgatatacat cacaattaag gaaatctact tacaagtttg catcactata atgcgatctg
    45361 gttcaaataa ttcactttca aatgaatgcg tcagtggtgg caaacgcatc aggatcaaag
    45421 tgaacatcac gaaacttctt acaatggcgc atcaccttcg gcataaaagg aaaagaagat
    45481 gcccacttct catgaaaatg cactgcaaca acgttgccag caaattgtca ccagcccagt
    45541 gcttagcccg gagcagaagc gccattttct ggcactggaa gcagaaaaca atctgcctta
    45601 cccacagctt cctgccgaag cccgccgcgc gctggatgaa ggtgtaatct gcgatatgtt
    45661 tgaaggtcat gcgccgtaca aaccgcgcta tgtcttaccc gattacgccc gttttctggc
    45721 gaacggttcc gaatggctgg agctggaagg cgcgaaagat cttgatgacg cactctctct
    45781 gctgaccatt ctttaccacc acgtaccgtc ggtcacatcg atgccggtct acctggggca
    45841 actggatgcg ttgttgcaac cgtatgttat aattctaaca caagacgaga tcgatgttcg
    45901 aataaaacgt ttctggcgtt acctcgacag aaccctgcca gacgccttta tgcacgccaa
    45961 tatcggcccg tctgattcgc ccattacccg cgcgatctta cgtgcagacg cagatttgaa
    46021 gcaggtttca ccgaacctga cctttatcta cgatcctgaa atcacccctg atgacctgct
    46081 gctggaagtg gcgaagaaca tctgtgaatg tagcaaaccg cacatcgcca acggtccggt
    46141 gcatgataaa attttcacaa aagggggcta cgggattgtg agctgttaca actcactgcc
    46201 gctggcgggt ggtggcagca cgctggtacg ccttaacctg aaagccattg ccgagcgcag
    46261 cgaatcgctg gatgacttct ttacgcgcac tctaccgcac tactgccagc agcagatcgc
    46321 catcatcgat gcgcggtgtg aattcctcta tcaacaatca cacttctttg agaatagctt
    46381 cctggtgaaa gaagggctga ttaaccctga acgttttgtg ccaatgtttg gcatgtatgg
    46441 gctggcggaa gcggttaact tgctgtgtga aaaagaaggg attgccgcgc gctacggtaa
    46501 agaagccgcc gcaaatgaag taggttatcg catcagtgca cagctggcgg agtttgtcgc
    46561 caataccccc gtgaaatatg gctggcaaaa acgcgccatg ttacacgcac agtcggggat
    46621 cagttccgat atcggcacca cgccgggcgc gcgtttgccg tatggcgatg agccagatcc
    46681 gatcacccat ctgcaaactg tcgcccgcat catgcttatt attattctgg catcagcgac
    46741 attctgacgc tcgacgaaac catcaaacgt aacccgcagg cgttggtaca gctttgcctc
    46801 ggtgccttta aagccggaat gcgtgaattt accgccaatg tcagcggtaa cgatctggtt
    46861 cgcgttaccg gttatatggt gcgtttgtcg gatttagaaa aatatcgcgc cgaaggttca
    46921 cgcaccaaca ccacctggct gggcgaagaa gccgcacgca acactcgtat tctggaacgc
    46981 cagccgcgcg tgataagcca tgaacagcag atgcgcttta gtcagtaaga ttatcccctt
    47041 ctcctgcgtt gacgggccag gcagtcgtct ggctctgttt ttgcagggct gcaatctgcg
    47101 ctgcaaaaac tgtcacaatc cgtggacgat gggacgttgc aatgactgtg gggagtgcgt
    47161 gccacagtgt ccgcatcagg cgttgcagat tgttgacggc aaagtggtgt ggaacgctgt
    47221 ggtctgcgag caatgtgata cctgcctgaa gaggtgtccg caacatgcca cgccaatggc
    47281 gcaatccatg agcgtggatg aggtgcttag ccatgtccgc aaagcagtgc tgtttatcga
    47341 agggataacg gtgagtggcg gcgaagccac gacccaactg ccgtttgtag tggcgctgtt
    47401 tactgctatc aaaaacgatc cgcaactgcg ccacctcacc tgcctggtgg acagtaacgg
    47461 tatgttgagc gaaaccggct gggaaaaatt gctcccggtg tgcgacggcg caatgctcga
    47521 tctcaaagcg tgggggagcg aatgtcatca gcaattaacc ggacgcgata atcagcagat
    47581 taagcgcagc atctgtttgc tggcagagcg cggtaagctg gcggaactgc gtttgctggt
    47641 aatccctggc caagtggatt atttgcaaca catcgaagaa ctggcggcgt ttatcaaggg
    47701 agtcggcgat gttccggtac gcctgaacgc gtttcatgcc cacggcgtgt atggcgaggc
    47761 gcaaagctgg gcgagcgcca cgccggaaga cgttgagccg ttggctgatg cgttacggga
    47821 gcgcggggtg agccggttaa tatttccggc gctgtatttg tgacatgccg gatacgacct
    47881 acggttcggc acagacttgt aggcctgata agacgcgtca agcgtcgcat caggcattgt
    47941 gcgccaactg ccggatgcgg cgtgaacgcc ttatccggcc tacggttcgg cacaaacctg
    48001 taggcatgat aagacgcgtt aagcgtcgca tcaggcatta tgcgccgact gccggatgct
    48061 gcctacgaag ggcaaactac ccacgaacgt taaacaccgc atacgtgtta ttaagcaaca
    48121 cttcggcaat ctcatcttct ggttccgggc gcaactcgca aagcacgtca aacacccgcg
    48181 cagcctgttc cgggcggtta ggctgcccct gaaaaccgtt gagcggcata tccggcgcgt
    48241 cggtttccag caacaacgat gccagcggta attttgcgat gacaacgcgg gttttactgg
    48301 cgcgtggata ggtgatagta ccgcctacgc cgattttgta gcccagctgt acaaaccgct
    48361 cggcctgttg caggctgccg gaaaaaccgt gaaccacgcc agtgcgcgat aaatcgtggc
    48421 gtttaagatg catcgccagt ttgtcgtgtg tgcgccgtga atgcaggatc accggcagat
    48481 cgtagcgttt cgccagtttc agttgttcgt cgagaaacca ctgctgcctc tcaaattgcg
    48541 gatcgtcgcc gaagagatcc agaccgctct cccccaccgc caccaccttc gccggacgcc
    48601 tttccagcgc ctgctgtagc tgatcaagag acacatcgct atgtttttcc aacataccag
    48661 gatgcaagcc caatgcggca tacagcggtt gataattttc cgctaacgcc tgcacgcggg
    48721 caaaattttc cgcctcggtt gccggaacaa tgatcttgcc tacgcccgct tgtgccgcgc
    48781 gttgcaggct ggcctcttca tcgccaataa aaggcgggaa atcaaaatgg cagtgggtgt
    48841 cgataaaacg gcaaatcaag ccagatcctc attgttaaat gtcgggtcgt tcgcctgcgg
    48901 agcgtcgctc acttcggcaa ccagcgtatc gttggcgact ggcgcaggcg gaatgacaat
    48961 cgattccggc gtcaccactg gcaccagatg gcgggtaagc ggcgcttttt cagttaatag
    49021 cttgccaacc gtggcgagga aataacgacc gcataaacgc ccaagtttat agtcttcacg
    49081 cagcgccgga atccgactgc caagcgcgat actatgtaat ggcttcggcg gataaatttc
    49141 gaatatccgc agcttgcccg gtggtttctc aataaattgc tgaatgtcac gatagctggt
    49201 ttcatgatgc tgcaccagat tgaccagcgg ctgcaggcta ctgtcaccca gccagcgttc
    49261 catgcgtttg aaccactgcg gcgtgtagta catttgtgac ggcacagtgc ggatgacgac
    49321 caacgtttta gcgccctgcc ttgccgcctc tttaaccgga atcgcatcac tgatcccgcc
    49381 atccaggtag ttaatgcctt ccagcgacac tccgctacga taaaagccag gtatcgcact
    49441 ggaggcgcga atcacatcca gccagttttg tttggttggt aaaaagtaat tcggcgcgta
    49501 gtcatcctga cgacaggcgc acatataaaa cgatttgccg ctgtcaaaca accgcgcggc
    49561 ggtatccatt tgcaacggca tctggcttgc ggtggcctcc accagccagt cgagatcgat
    49621 aagatttcct ccacggacaa agcgcaatgg atcgaaaaat tcgcgttttg tggtatagcg
    49681 catgatgact ttgcgcgcgt aaccgggctg attgcagata tacgccgaga ggttctgcgc
    49741 cccggcagat gtgccgagat aaagatcgaa aggattaaac tgcgcgcgca taaactcatc
    49801 cagcacgcca gccgtgaaaa ttccacgctg tccgccgcct tcgcacacca gagctattcg
    49861 tccaggctgg aacggcctta gcgacaacgg cgcaatatta ccaagcgtta caggtattcg
    49921 ctgccccacc tctgccttcc tgtttttgtt gtgaaagaat gacaaagtaa cgcaatttat
    49981 tattcgctaa aaccgcaaaa gccagtccgc tggactggct ttggataaaa ttttcttaat
    50041 ggtctgaaat ctagggtcgt tttcggccca tgaacaaact caccaggaac agaataatcc
    50101 cgacgacaaa gacaatttta gctgcgcctg cagcggtacc ggccagacca ccaaacccaa
    50161 gtgcggcggc gattaacgcg ataaccagaa atatgatgcc ccaacgaaac ataagcgtct
    50221 cctttaccat agttaatgtc accgcttgat atgagcgtga aaatcactca atgcgatatt
    50281 tttagtgtgg tgcacatcgc gcctcccgac aaagttcggg aggacgaatt acgacgaatt
    50341 acttagtttt cagatcattt ttaacgcttt tcacaccatc taccgctttg gcgatacttt
    50401 cagcacggtc actttgtgcc tgagaatcta cgataccaga gagctgaacc acgccatcgg
    50461 tggtttcaac tttcacatga cgggaaggga cgatatcgtc cgccagcaat ttggctttga
    50521 tttcactggt ggtggcggtg tcacccgcgt agcccttcac agagccttct ttagcgtcgc
    50581 gaacgtgcag tttgtcgctg acagaggtca ccccttcaac acctttcgcc actttcactg
    50641 cctcttcggc ctgggcctgg ctttcaacga aaccgctcag ggtcacgact ttttgatcgg
    50701 ttttgacaga gatatcggtg ctcttgatgt tgtcatgatc caccagcgcc gccttcactt
    50761 tcgcggtgat ggcgctgtca tccatgaaat taccgacttt attcatagag ctatcgactt
    50821 tttgccctgc gctttcattg gtagtctgcg cgttgttttc cgcgtaggca gagccggtcg
    50881 cgacggcaga ggtcaacatt acagccagca gagttttcga aatcttcagt cttgtcatag
    50941 tcatcgattt attcctgtat gtttgctcgt aatttgagcc tggcaacacg aggttgcatt
    51001 gctgaatagg gagagacttc accctctaca gaagtcaatg gtcgccatca caaaagcgat
    51061 gagtgatgaa taacgaccat tacagcctct gaatcagtta ttaatatcgg tagaatgaca
    51121 atcgacggct ttagatactg atatctacgc attgaacggt atttaacgcc gtcagaaatg
    51181 tcatcacttt gttaaatata gatcacaatt ttgaaaccgc tcgggatatc agcgaaaaca
    51241 taagcaaaag tgaatgtttt aagaacattc cgtaagcggc taataaggaa gggaaattga
    51301 caggacgcag cggctaccgc gccctggagg caagaattaa tgctcgcggg tctggtggaa
    51361 ctgaacgtcc ggataacgtt cctgtgccag gcgcaggttg accatgctgg tagcgatgta
    51421 agcgaggtta tcgccgccat caagcgccag ttggctttcg ttcttacgct tgaactcttc
    51481 gaatttcttc gcgtctgcac attctaccca gcgggcagtg gcaacgttga ctgactcata
    51541 cactgcttca acgttgtatt cgctcttcag gcgcgctacc accacatcaa actgcagcac
    51601 accaaccgca ccaacgatca gatcgttgtt ggagattgga cggaacacct gcaccgcgcc
    51661 ctcttcggaa agctgtacca gccctttgag cagctgtttt tgcttcagcg gatctttcag
    51721 gcggatacga cggaacagtt ctggtgcgaa gttcggaata ccggtgaact tcatcatctc
    51781 accctgggta aaggtgtcgc cgatctgaat ggtgccgtgg ttgtgcaggc cgaggatatc
    51841 gcccggatac gcttcttcaa cgtgcgaacg gtcacccgcc ataaaggtca gcgcgtcgga
    51901 gatcaccaca tctttcgcag tgcgcacctg gcgcagcttc atgccttttt catatttacc
    51961 ggacaccaca cgcataaacg ccacgcggtc gcggtgtttc gggtccatgt tggcctgaat
    52021 tttaaatacg aagccggtaa atttatcttc gctcgcttct acggtacggg tatcggtctg
    52081 acgcggcatc ggcgcaggcg cccactccac caggccatcc agcatatgat caacgccgaa
    52141 gttacccagc gcagtaccga agaataccgg agtgatttcg cccgcaagga acagctcttt
    52201 gtcgaactcg ttagacgcgc ctttcaccag ttccagttcg tcacgcagct gctgtgccag
    52261 atcttcacca accgcagcat cgagatccgg gttattcagc cctttaacaa tgcggacttc
    52321 ctgaatggtg tgccctttac cgctctgata gagataggtt tcatctttat aaaggtggta
    52381 aacgccttta aacagcttgc cgcagccaat tggccaggtg atcggtgcgc agccaatttt
    52441 cagctcgttc tcaacttcat cgagcaattc catcgggtcg cggatatcac ggtcaagttt
    52501 gttcataaag gtgaggatcg gcgtgtcgcg cagacgggta acttccatca gcttacgggt
    52561 acgatcttca acaccttttg cggcgtcgat aaccatcagg cagcagtcca ccgccgtcag
    52621 ggtacgatag gtatcttccg agaagtcttc gtgccccggg gtgtcgagca ggttaaccag
    52681 gcaatcgtga tacggaaact gcatcacaga cgtagtaatg gagatcccac gctgcttttc
    52741 catctccatc cagtccgact tagcgtgctg gttggaacca cggcctttta ctgtaccggc
    52801 ggtctgaatg gcctgtccga acagcagcac cttctcggtg atggtagtct taccggcgtc
    52861 cgggtgagaa ataatggcaa aagtgcggcg cttcgccacc tcttgcaaat aaggagacaa
    52921 cgtcataatt ctttcttctt gagtaagcgg catcgcgccg cgcttgttga aaacgaaaaa
    52981 ttgcggctat tttacccatc aacggggggg aggcaatcag tgtttacaca ggagctgctc
    53041 cagttcgtgc aacgaagaaa cggtccaggt gggcgcgatg ccttctggtt gctcgcgatg
    53101 gtgtgcattc agccagcagg tcgcaagccc ggcgttgatg ccaccgagaa tatcggactc
    53161 ggcagtgtcg ccaaccatca gcacgcgtga acggtcagga ttgcccgcct gttccagcgc
    53221 ataatcgaaa attttcttat tcggtttggc aacgccaact tcttcggaaa tcaccagcaa
    53281 atcgaagtaa tcacgcaggc ccgtgcgttc cagacgcacc tgttgcaagg cactaaagcc
    53341 gttggtgatg atgccgattt tggcgttgcc acgaatggcg ttaagcagag aaaccgcgcc
    53401 cggcagcggc gtgcagattt ccgccatcgc attaataaag gcttcgttga gtttacctgg
    53461 ctcgacgttc agccgttcgg cccagctctc aaaccgcccg tgctgaagct gtaatgaagt
    53521 gatcgcgccg ttttgataat ccacccacag tggcttgtta acggcctgat agtcctgaaa
    53581 atcttcagcg gtaaaggtga cgctgtaatc aagaaacatc cgctgcaggc cggtgaatga
    53641 gtcaaaggta aacagcgttt catcggcatc aaagaaaatc cagtcccact tcattattcc
    53701 accttgtctt acatactgat tggcaacgcc atgatgatgg cgtcttcgcg accgtccgtg
    53761 gtggggtagt aattgcggcg aatcgtcgcc tcgttaaagc ctaaactttc gtacagggca
    53821 atggcggcag catttgaggc acggacttcc agccatagtg tcgccacgcc gcgtttttcc
    53881 agttcgtcga tcagatgttc cagcagcgcc cttcccaatc cctgacgctg atagtcagga
    53941 tcgaccgcaa tattgaacaa tgtagcttca tccagcacca cttgcgtaat cgcaaacgcc
    54001 gccattttgc cgttttgcgt taactgaaag ttgagataac gctcgccctg gttgctggca
    54061 aaggtttttt cactccacgg aaaggcgtgg gcgcgttgtt caatgtggta agccgctggt
    54121 aaatcagtcg tttcgaggga agaaatcgtg ttcatatgtg caaatttgtt gccataacgc
    54181 ggcgcgtgcc gttgggtttg cccgtaattc ggtgagcgcc ggtgatgcca cctgagcgcc
    54241 ttccagtgat agcggttcgt cagtacccaa ccgccagctg ttgcagcgac tgccttgcgg
    54301 cagcatcgcg attttttctg gcgtcagttg cagcacttga tcggggctga cggttaatgc
    54361 gcgcagaaca tcgctcacta aaggatcagt cagggcggga agatcgtttg ccaccatcac
    54421 cagacggacg tgtgccggga tcgcaatggc aatctccccc tgcaacgcgc cagggcgacg
    54481 cagcgaccac tgggtaatgc ccagttgctg taactgccag tctcgtcggg atgtcatagc
    54541 gaatcgctcc tgttgtcagg ggcgcaaata tagcaaattc gtcgataccg cgccaacata
    54601 tggctataat cgccgccagt atcaattgag gagcattcca tgtctgcatt taccccggca
    54661 agtgaagtct tgctgcgtca cagtgatgat ttcgaacaaa gccgtattct gtttgccgga
    54721 gacttacagg atgacctgcc cgcgcgttta gataccgcgg ccagccgtgc tcatacccag
    54781 caattccagc attggcaggt gttaagccgc cagatggggg ataacgcccg ttttagtctg
    54841 gtcgccacgg cggatgatgt cgcagattgc gatacgctga tttactactg gccgaagaac
    54901 aaaccggaag cccagttcca gttgatgaat ttactttctc tgcttccggt ggggacggat
    54961 atttttgttg ttggcgagaa ccgcagcggc gtgcgcagcg ccgagcagat gctggctgat
    55021 tatgcgccgt tgaataaagt cgacagcgcc cgtcgctgtg gcctctattt tggtcgtctg
    55081 gaaaaacagc cggtatttga tgccgataaa ttctggggcg aatacagcgt cgatggcctg
    55141 acggtcaaaa cgctgcctgg cgtgtttagc cgcgacggtc tggatgtcgg tagccagttg
    55201 ctgctctcga cgttaacccc gcacacgaaa ggtaaagtgc tggatgtcgg ctgtggcgcg
    55261 ggcgtgcttt cggttgcctt tgcgcgccat tcgccgaaaa ttcgtctcac cttgtgcgat
    55321 gtctctgcgc cagcggtaga agccagccgc gcaacacttg cggccaacgg tgttgaaggt
    55381 gaagtctttg ccagcaacgt cttttccgag gtgaaaggtc gttttgatat gatcatctcc
    55441 aacccgccgt tccacgatgg gatgcaaacc agcctggatg cggcgcaaac gctgattcgc
    55501 ggtgcggtgc gtcatcttaa tagcggcggc gagctgcgaa ttgtagcgaa cgccttcctg
    55561 ccttatccgg acgtgctgga tgagacattt ggcttccacg aagtgatcgc gcaaacaggg
    55621 cgcttcaagg tgtatcgcgc cattatgacc cgccaggcga agaaaggtta attatctcgc
    55681 cgaataccgt gtcggatgcg gcgtgaacgc cttatccgac ttacaaaacc tgcacgttag
    55741 tctcctgatg gacatttttc cagcaattac acatcaggcg ataattaact attgacgaaa
    55801 agctgaaaac cactagaatg cgcctccgtg gtagcaattc tttttaagaa ttgatggtat
    55861 gcgaaggtgg cggaattggt agacgcgcta gcttcaggtg ttagtgtcct tacggacgtg
    55921 ggggttcaag tcccccccct cgcaccaatt atctttactt cctttcttgt ttcttccctg
    55981 ttttttcatc cctgattcaa attcattatc atcagcatga tgccagcaat aagcgccagg
    56041 cacgatggta gcaaaatgta atgccgcaac tgtttgtgat acagcatcac cggcgtcaat
    56101 actaatccca tcagaatgac aatccgccac tgatccggcg ataaatccgc atacatcagc
    56161 cctgcaatca gaaatcccgg cagcaacact ccccagccac tgcccagcgt acgttgcaac
    56221 atggttttat ctctttcatt gataatgata accaatatca tatgataatt tttatcactt
    56281 gcaagccaga gaaatccctt gctatcgggt aaacctatcg ctaggattag caatcattat
    56341 catttagatt actatcccga ttatggccta tcgttccgca ccgctctatg aagatatcat
    56401 ctggcgaacg catctccagc cacaggatgc taggcttgca caagccgtac gtgcgacgat
    56461 cgccgaacat cgtgaacatt tgctggagtt tatccgcctg gatgaacccg ctccgcttaa
    56521 cgccatgacg ctggcgcaat ggtcatcacc gaatgcgcta agttctctac tggcggtcta
    56581 ttccgatcat atctatcgca accaaccaac gatgatccgc gagtacaaac cgctgatctc
    56641 actatgggca caatggtata tcggcctgat ggtgccacca ttaatgctgg cgctactgac
    56701 gcaggaaaaa gcattagatg tgtcgccgga acatttccat gctgagtttc acgaaaccgg
    56761 acgcgtcgcc tgtttctggg tcgatgtgtg tgaagataaa aacgcaacac cacattcgcc
    56821 gcagcagcga atggaaacgt taatcagcca ggcgctggtt ccggttgtgc aggcactaga
    56881 agcgaccgga gagatcaacg gcaaacttat ctggagtaat accggttatt tgatcaactg
    56941 gtatcttact gagatgaagc aactgctcgg cgaggcgacg gttgaatcgc tgcgccatgc
    57001 cctctttttt gagaaaacgc ttactaccgg tgaagataat ccactctggc gtaccgtggt
    57061 gctacgcgac ggcctgctgg tgcgccgcac ttgctgccag cgttatcgct taccggatgt
    57121 acagcaatgt ggcgattgta cgctgaaatg aaagcactgc cggggaagta aacccggcat
    57181 catgcggatt aatagggatg caacacatta cttgtttcgc aatgccgcat acataacaga
    57241 atatctgcgg cctccaacaa ccctgcgtca gaactgacgc ccagtttcga catcacatta
    57301 aatttatgtg cacggatcgt tttgatattg cgtttaagct gctcggcaat ttgtgtcatt
    57361 gagtagccac gcgacataaa gcgcaatatt tctctctccg tcgggcttaa cgtccggctt
    57421 tggttaatgt accactgatt gttcagtcgg tcggtcgcct gacgtacacc atttaatgac
    57481 aaaaagagtt cctgatgaaa aatctccagc gttgacgctt tacttaatac accgtccagc
    57541 ggtgatggcg acaatgaacc aatcagccga gcttcaatat catcatccgc aataactaaa
    57601 cgccgggtac gcggaaactt aatcgccagt tcagtcaggc aagataatcc ctctctgcgc
    57661 tcacttctca tggcagaaaa agaaaaaatg accgccgcaa acgagatccg cgacatggca
    57721 tcctgaaatg actcctgact ggtataggta tggagcttat aagggttgcc cgcaaacttt
    57781 ctgaataaac tctcaatacc gatactactc atgacgcatt tttctatgag tgcgacattt
    57841 ctcttcttaa ttcggctgtg ttccattctg cggcatctcc ataaacagaa ggcaagttaa
    57901 gttctcttgc accctgcgcg ctatttatcc atgcatacat ttcagcgttg ctgtgaattg
    57961 ccagtcgacg catcgcactg tttttttggg cgctgatagt tttattactt ttcttaagca
    58021 atgaagctat ctggttgatg ccccatcctt tacctaatag ccgcagtacc ttgcgctctg
    58081 aaagagtgag aatgacggag cgatagctaa aatcatgagt ttcagtcatt gccggagagg
    58141 tcagcatggc gctgatacgc tctgcgtgcg tattgccgga acgtacggtc ttgaccagat
    58201 tctcaatggg ttcaacatca gacaataacg tcgccgtagg gcacatgagc agttctacag
    58261 cctgggaata ccaagagcgt gacaccatga aaacccagtg aatttcccga tattgtgaga
    58321 ttaaagaata ataatgttca caaacagaac gtggatcttc actttcaccg gctaaatcag
    58381 caatgactaa tccggaacga cgtaattgta ataaggtaag gtcctccgca gaggcgctgg
    58441 atattatttc atattcaggg aagtgagtcc tcatgacctc ttttaaccct gcttgcataa
    58501 caggtatttt actgataaca attccatttt tgcagcatcc tggcaacata tttcctccac
    58561 atccactgtc taaaaattat gatggtttat tcaggatatt cattccttaa atgtttccag
    58621 caaattaata gattaagcga ataaattatc cttagcattc actttcatat cagcatactc
    58681 ctgctgcaca acaactccac tgcatgtgtc agaaggttag catcaaatga taacctggtg
    58741 aaatagcctg gagacattta aaatatctac attaagttgt tagaaatact taatgaaaaa
    58801 cag
//
LOCUS       DAZKIP010000017        51292 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZKIP010000017 DAZKIP010000000
VERSION     DAZKIP010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.017"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..242
                     /locus_tag="ACHOUK_001266"
     CDS             <1..242
                     /locus_tag="ACHOUK_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFP9896743.1"
                     /translation="NNTTDFRPDPEDIFKCGQTLECGDPRKNIRQKYGHHGLGVNQNQ
                     QFDNGVMGLNSRMAVFDENKKIIGSSSGLSCFQPH"
     gene            504..2126
                     /gene="yhiJ"
                     /locus_tag="ACHOUK_001267"
     CDS             504..2126
                     /gene="yhiJ"
                     /locus_tag="ACHOUK_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFP9896744.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVLEKVDDWQIERFQQSIQDDKISFTIQTDYSEKYSMLSGMRAHILRR
                     NNHYQFIVTINSKNYGCPLDNTDINWCGIVYLLNNMTVNDNANDVAVTESYKPIWNWK
                     ISQYNVSDIKFETIIKPQFADRIYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQVDTNVNTIISILNRRDIILESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINDNVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDSKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAAIINKKHHTIINSKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHTGRMFSMHNCQINPSA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            2316..2429
                     /gene="yhiY"
                     /locus_tag="ACHOUK_001268"
     CDS             2316..2429
                     /gene="yhiY"
                     /locus_tag="ACHOUK_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFP9896745.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            2492..3559
                     /gene="yhiI"
                     /locus_tag="ACHOUK_001269"
     CDS             2492..3559
                     /gene="yhiI"
                     /locus_tag="ACHOUK_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFP9896746.1"
                     /translation="MDKSKRHLAWWGVGLLAVAAIVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            3556..6291
                     /gene="rbbA"
                     /locus_tag="ACHOUK_001270"
     CDS             3556..6291
                     /gene="rbbA"
                     /locus_tag="ACHOUK_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFP9896747.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVIVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVIHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPLLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPSSQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            6291..7415
                     /gene="yhhJ"
                     /locus_tag="ACHOUK_001271"
     CDS             6291..7415
                     /gene="yhhJ"
                     /locus_tag="ACHOUK_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9896748.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLIMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            7488..7763
                     /locus_tag="ACHOUK_001272"
     CDS             7488..7763
                     /locus_tag="ACHOUK_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFP9896749.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            7760..8119
                     /locus_tag="ACHOUK_001273"
     CDS             7760..8119
                     /locus_tag="ACHOUK_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFP9896750.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(8169..9029)
                     /locus_tag="ACHOUK_001274"
     CDS             complement(8169..9029)
                     /locus_tag="ACHOUK_001274"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase"
                     /protein_id="HFP9896751.1"
                     /translation="MPLVNGRILLDRIQEKRVLAGAFNTTNLETTISILNAIERSGLP
                     NFIQIAPTNAQLSGYDYIYEIVKRHADKMDVPVSLHLDHGKTQEDVKQAVRAGFTSVM
                     IDGAAFSFEENIAFTQEAVDFCKSYGVPVEAELGAILGKEDDHVSEADCKTEPEKVKT
                     FVERTGCDMLAVSIGNVHGLDDIPRIDIPLLKRIAEVCPVPLVIHGGSGIAPEILRSF
                     VNYRVAKVNIASDLRKAFITAVGKAYVNNHNEANLARVMASAKNAVEEDVYSKILMMN
                     EGHRLVRKAS"
     gene            complement(9061..9330)
                     /locus_tag="ACHOUK_001275"
     CDS             complement(9061..9330)
                     /locus_tag="ACHOUK_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFP9896752.1"
                     /translation="MLNKTVEVRNSTGLHARPAACLAKAAKKYSCKVTLHYEGNDINA
                     TSMMNIMRAGIKGGKTVEIRCDGDDENEAIQTLTDLFRDRFGEAE"
     gene            complement(9320..10828)
                     /locus_tag="ACHOUK_001276"
     CDS             complement(9320..10828)
                     /locus_tag="ACHOUK_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFP9896753.1"
                     /translation="MPDNSAAIVIDIGTTNCKVTCFSCLDATTLGAHKFVTAKQISPQ
                     GDVDFDIDALWQEVRQAIAQLNAASPLPVRRISIASFGESGVFLDEHGEILTPMLAWY
                     DRRGEEYLATLSEADSAALYDICGLPLHSNYSAFKMRWLLEHYPLRNRRGLRWLHAPE
                     VLLWRLTGEQHTDITLASRTLCLDVRKGEWSAKAAALLHVPCTAFAPLVQPGEHAGWV
                     SESLCKTLGFSQPVSVTLAGHDHMVGARALQMMPGDILNSTGTTEGILQLDTQPTLDE
                     QAKRDKLANGCYSLANQFTLFASLPVGGFALEWLRNTFRLTDEEIAASLTRGHADYLA
                     GNWLLDDIPVFIPHLRGSGSPYKNRHTRGLFYGLGDTLSIDMLIASVSLGLTMEFANC
                     FACFNVPGTSALKVIGPATHNPLWLQLKADILQRPVEAIAFNEAVSVGALLTAAPDIP
                     PPPVAIAQRLLPNRARYHQLQRYQHKWKSWYQLKLQQEGVMPLHHREEHYVE"
     gene            complement(10821..12179)
                     /locus_tag="ACHOUK_001277"
     CDS             complement(10821..12179)
                     /locus_tag="ACHOUK_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFP9896754.1"
                     /translation="MNDIAHTLYNIVQYILGFGPTVMLPLVLFILALCFKVKPAKALR
                     SSLTVGIGFVGIYAIFDILTSNVGPAAQAMVERTGINLPVVDLGWPPLSAITWGSPIA
                     PFVIPLTILINVAMLALNKTRTVDVDMWNYWHFALAGTLVYYSTGSLFFGLLAAAIAA
                     VVVLKLADWSAPLVQKYFGLEGISLPTLSSVVFFPVGLLVDKIIDHIPGLNRIHIDPE
                     TVQKKFGIFGEPMMVGTILGILLGVIAGYDFKKVLLLGISIGGVMFILPRMVRILMEG
                     LLPLSEAIKKYLNAKYPDRDDLYIGLDIAVAVGNPAIISTALLLTPISVFIAFVLPGN
                     EVLPLGDLANLAVMASMIALASRGNIFRTVLAAIPVIIADLWIATKIAPFITGMAKDV
                     NFKFAEGSSGQVSSFLDGGNPFRFWLLEIFNGNLIAIGLVPVIALVLYGIFRMTRSTV
                     YA"
     gene            complement(12256..12537)
                     /locus_tag="ACHOUK_001278"
     CDS             complement(12256..12537)
                     /locus_tag="ACHOUK_001278"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9896755.1"
                     /translation="MSQTILFVCATGIATSTAVTEKVMEYCKEQGLNVNYSQTNVASL
                     PGNTDGVALVVSTTKVPYELDVPVVNGLPIITGIGEEKVLAQIVSILKK"
     gene            complement(12534..13007)
                     /locus_tag="ACHOUK_001279"
     CDS             complement(12534..13007)
                     /locus_tag="ACHOUK_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9896756.1"
                     /translation="MQGIHFQENYIQRLPAGLSVEQIIRQLAQPLVAAELVVPDFADH
                     VLKREATYPTGLPTEPPCVAIPHTDHKHVRHNAIAVGILPEPVEFADMGGDPDPVPVR
                     VIFLLALSESNKQLNALGWIMEMIQDTPFMQALLAMETTEIHTVILNKMKERGEI"
     gene            complement(13032..13778)
                     /locus_tag="ACHOUK_001280"
     CDS             complement(13032..13778)
                     /locus_tag="ACHOUK_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9896757.1"
                     /translation="MKSLSKSSQIPLYQQVVEWIRESIYTGDLVEDDRIPSEYQIMDM
                     LDVSRGTVKKAVAQLVKEGVLIQVQGKGTFVKKENVAYPLGEGLLSFAESLESQKIHF
                     TTEVITSRIESANRYVAEKLRITPGQDILYLERLRSIGDEKAMLIENRINIELCPGIA
                     EIDFNQHNLFPTIESLSQRKIRYSESRYAARLIGNERGHFLDISEDAPVLHLEQLVFF
                     SRGLPIEFGNVWLKGNKYYLGTVLQRREVS"
     gene            complement(13977..14378)
                     /gene="nikR"
                     /locus_tag="ACHOUK_001281"
     CDS             complement(13977..14378)
                     /gene="nikR"
                     /locus_tag="ACHOUK_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFP9896758.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(14384..15190)
                     /gene="nikE"
                     /locus_tag="ACHOUK_001282"
     CDS             complement(14384..15190)
                     /gene="nikE"
                     /locus_tag="ACHOUK_001282"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFP9896759.1"
                     /translation="MTLLNVSDLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLSRLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLHAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQAVGEKLTFSSDAGRVLQNAILPAFPVRRRATEKV"
     gene            complement(15187..15951)
                     /gene="nikD"
                     /locus_tag="ACHOUK_001283"
     CDS             complement(15187..15951)
                     /gene="nikD"
                     /locus_tag="ACHOUK_001283"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFP9896760.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(15951..16784)
                     /gene="nikC"
                     /locus_tag="ACHOUK_001284"
     CDS             complement(15951..16784)
                     /gene="nikC"
                     /locus_tag="ACHOUK_001284"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFP9896761.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(16781..17725)
                     /gene="nikB"
                     /locus_tag="ACHOUK_001285"
     CDS             complement(16781..17725)
                     /gene="nikB"
                     /locus_tag="ACHOUK_001285"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFP9896762.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFATQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFTWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(17725..19299)
                     /gene="nikA"
                     /locus_tag="ACHOUK_001286"
     CDS             complement(17725..19299)
                     /gene="nikA"
                     /locus_tag="ACHOUK_001286"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9896763.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWVLQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLHIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDEAQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(19410..19997)
                     /gene="acpT"
                     /locus_tag="ACHOUK_001287"
     CDS             complement(19410..19997)
                     /gene="acpT"
                     /locus_tag="ACHOUK_001287"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFP9896764.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(19999..21228)
                     /locus_tag="ACHOUK_001288"
     CDS             complement(19999..21228)
                     /locus_tag="ACHOUK_001288"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HFP9896765.1"
                     /translation="MTRRVVITGMGGVTAFGENWQDVSARLLAYENAVRKMPEWQVYD
                     GLHTLLGAPVDDFTLPEHYTRKRIRAMGRVSQMSTRASELALEQAGLIGDPILTSGET
                     GIAYGSSTGSTGPVSEFATMLTEKHTNNITGTTYVQMMPHTTAVNTGLFFGLRGRVIP
                     TSSACTSGSQAIGYAWEAIRHGYQTVMVAGGAEELCPSEAAVFDTLFATSQHNDAPKT
                     TPSPFDENRDGLVIGEGAGTLILEELEHAKARGATIYGEIVGFATNCDAAHITQPQRE
                     TMQYCMEQSLKIAGLSAQDIGYISAHGTATDRGDMAESLATATIYGDNVPLSSLKSYF
                     GHTLGACGALEAWMSLQMMREGWFAPTLNLNKPDPNCGALDYIMHEARKVDCEFLQSN
                     NFAFGGINTSIIIKRWP"
     gene            complement(21225..21956)
                     /locus_tag="ACHOUK_001289"
     CDS             complement(21225..21956)
                     /locus_tag="ACHOUK_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase FabG2"
                     /protein_id="HFP9896766.1"
                     /translation="MSRSVLVTGASKGIGRAIACQLAADGFNIGVHYHRDATGAQETL
                     NAIVANGGNGRLLSFDVANREQCREVLEHEIAQHGAWYGVVSNAGIARDAAFPALSDD
                     DWDAVIHTNLDSFYNVIQPCIMPMIGARQGGRIITLSSVSGVMGNRGQVNYSAAKAGI
                     IGATKALAIELAKRKITVNCIAPGLIDTGMIEMEESALKEAMSMIPMKRMGQAEEVAG
                     LASYLMSDIAGYVTRQVISINGGML"
     gene            complement(21956..22420)
                     /locus_tag="ACHOUK_001290"
     CDS             complement(21956..22420)
                     /locus_tag="ACHOUK_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-fatty acyl-ACP dehydratase"
                     /protein_id="HFP9896767.1"
                     /translation="MSHYLSPGAYLPHDAPMLLLEDVVSVSDDSAVCRVTVSPSGVLA
                     PFLDPDGNLPGWFTLELMAQTVGVWSGWHRHQQGKNSIELGMILGARELLCTTGILPA
                     GQTLTITVKLLMQDDRFGSFECSINDGEATGRINTFQPTAEELTTLFQQGAS"
     gene            complement(22417..23586)
                     /locus_tag="ACHOUK_001291"
     CDS             complement(22417..23586)
                     /locus_tag="ACHOUK_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HFP9896768.1"
                     /translation="MIYISAVGMINALGNNLDEIATNLTRGVAPGMRPRAGWLQGHPQ
                     AVLAGVDGELPLIPEKFAAHRSRNNQILLAALAQIQPQVDDAIAKYGRERIAIVLGTS
                     TSGLHEGDTHVNLRTHGQPSTTWHYAQQELGDPSRFLSHWLALDGPAYTLSTACSSSA
                     RAIISGRRLIEAGLVDAAIVGGADTLSRMPINGFHSLESLSPTLCQPFGRDRAGITIG
                     EGAGLMLLTREPQPIALLGVGESSDAYHISAPHPQGEGAIRAINQALTDAQLTPDDVG
                     YINLHGTATQLNDQIESMVVNALFGERVPCSSTKHLTGHTLGTAGITEAAISMLILQR
                     DLPLPAQDFSLSPRDPTLPPCGIIEKPQPLARPVILSNSFAFGGNNASILLGRVS"
     gene            complement(23588..24172)
                     /locus_tag="ACHOUK_001292"
     CDS             complement(23588..24172)
                     /locus_tag="ACHOUK_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3261 domain-containing protein"
                     /protein_id="HFP9896769.1"
                     /translation="MIKSTFWRAFALTATLILTGCSHSQPEQEGRPQAWLQPGTRITL
                     PAPGISPAVNSQQLLTGSFNGKTQSLLVMLNADDQKITLAGLSSVGIRLFLVTYDAQG
                     LRAEQSIVVPQLPPASQVLADVMLSHWPISAWQPQLPAGWTLRDNGDKRELRNASGKL
                     VTEITYLNRKGKRVPISIEQHVFKYHITIQYLGD"
     gene            complement(24169..26487)
                     /locus_tag="ACHOUK_001293"
     CDS             complement(24169..26487)
                     /locus_tag="ACHOUK_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HFP9896770.1"
                     /translation="MTNANVLPPSKRPALLWGLVCLVMAVALLILLPQSRLNSSVLAM
                     LPKQTMGDIPPALNDGFMQRLDRQLVWLVSPGKEANPLVAQEWLTLLQKSAALGDVKG
                     PMDAASQQAWGAFFWQHRNGLIDPNTRARLQNGGEAQAQWILSQLYSAFSGVSGKELQ
                     NDPLMLMRGSQLAMAKNGQRLRLMDGWLVTQDPQGNYWYLLHGELAGSSFDMQQTHQL
                     ITTLNTLEKDLKTRYPQAQLLSRGTVFYSDYASQQAKQDISTLGVATLLGVILLIVAV
                     FRSLRPLLLCVISIGIGALAGTVATLLIFGELHLMTLVMSMSVIGISADYTLYYLTER
                     MVHGNDVSPWQSLAKVRNALLLALLTTVAAYLIMMLAPFPGIRQMAIFAAVGLSASCL
                     TVLFWHPWLCRGLPVRPVPAMALMLRWLAAWRRNKKLSLGLPVALALFSLAGMSMLRV
                     DDDISQLQALPQHILAQEKAITALTGQSVDQKWFVVYGDSPQQTLRRLEKYTASLEYA
                     KKEGLISNYRTIPLNSLARQEEDLQLLKTAAPTVTKALQNAGLTAVNPDLNAMPVNVD
                     EWLASPASEGWRLLWLTLENGESGVLVPVEGVKSSALMQEIATYYPCGIAWVDRKSTF
                     DELFALYRYVLTGLLLVALAVIACGAVARLGWRKGLISLVPSVLSLGCGLAVLAMSGQ
                     AVNLFSLLALVLVLGIGINYTLFFSNPRGTPLTSLLAIALAMLTTLLTLGMLVFSATQ
                     AISSFGIVLVSGIFTAFLLSPLAMPDKKRTKK"
     gene            complement(26456..27061)
                     /locus_tag="ACHOUK_001294"
     CDS             complement(26456..27061)
                     /locus_tag="ACHOUK_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HFP9896771.1"
                     /translation="MKFLPLLALLISPFVSALTLDDLQQRFTEQPVIRAHFDQTRTIK
                     DLPQPLRSQGQMLIARDQGLLWDQTSPFPMQLLLDDKRMVQVINGQPPQIITAENNPQ
                     MFQFNHLLRALFQADRKVLEQNFRVEFADKGEGRWTLRLTPTTTPLDKIFNTIDLAGK
                     TYLESIQLNDKQGDRTDIALTQHQLTPAQLTDDERQRFAAQ"
     gene            complement(27058..27480)
                     /locus_tag="ACHOUK_001295"
     CDS             complement(27058..27480)
                     /locus_tag="ACHOUK_001295"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFP9896772.1"
                     /translation="MLNDPRFTAEVELTIPFHDVDMMGVAWHGNYFRYFEVAREALLN
                     QFNYGYRQMKESGYLWPVVDARVKYRHALTFEQRIRVRAHIEEFENRLRIGYQIFDAE
                     TGKRATTGYTIQVAVDEQSRELCFVSPDILFERMGVKP"
     gene            complement(27484..29160)
                     /locus_tag="ACHOUK_001296"
     CDS             complement(27484..29160)
                     /locus_tag="ACHOUK_001296"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP9896773.1"
                     /translation="MSVNFSPCVLIPCYNHGAMMPGVLARLKPFNLPCIVVDDGSDAT
                     TQQQLDSLLAEQPGVTLIRLAENAGKGAAVMRGLQAAADAGFSHAVQVDADGQHAIED
                     IPKLLALAEQQPAALISGQPIYDDSIPRSRLYGRWVTHVWVWIETLSLQLKDSMCGFR
                     VYPVAPTLQLAKHATIGKRMDFDTEVMVRLYWQGNTSYFVPTRVTYPLDGLSHFDALK
                     DNVRISLMHTRLFFGMLPRIPSLLMRRSSCHWARQSEVKGLWGMRLMLLVWRLLGRTA
                     FSALLYPVVGVYWLTASRARKASQDWLARVRQHQPQAAKLNSYQHFLRFGNAMLDKIA
                     SWRGELQPGRDVLFAPGAEAALDVRDPRGKLLLASHLGDVEVCRALAKIQGYKTINAL
                     VFSENAQRFKQIMQEMAPQAGINLMPVTDIGPETAILLKEKLDNGEWVAIVGDRIAVN
                     PQRGGDWRVCWSSFMGQPAPFPQGPFILASILRCPVNLIFALRQHGKLHIHCESFADP
                     LQLPRGERQQALQNAIDHYAARLEHYALQSPLDWFNFFDFWQLPEIQDKE"
     gene            complement(29151..29504)
                     /locus_tag="ACHOUK_001297"
     CDS             complement(29151..29504)
                     /locus_tag="ACHOUK_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HFP9896774.1"
                     /translation="MKPHEIERHQAQTNHLEIVLHLRADLFWFRGHFAVQPLLPGVAQ
                     IDWAMSYALNLLAPGWRFHSIQNIKFQSPLLPENRVTLALNWQEERQILSFSYQRHDG
                     DARHTASSGKIRLCR"
     gene            complement(29491..30852)
                     /locus_tag="ACHOUK_001298"
     CDS             complement(29491..30852)
                     /locus_tag="ACHOUK_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase"
                     /protein_id="HFP9896775.1"
                     /translation="MKQTLPLARWLTAPRPDDTPIAWLNESTWTLGDLRHDVAQLICR
                     LQQQPGERWALCFENSYLFIVALLATLHAGKTPVLPGHNRVIQLNEQRELFDGVLSDS
                     ELNWQGSLLLVASSPQIATQSFTFAAIAPEAFIELFTSGSTGQPKRAIKPVHLLDREA
                     ELLAERLGARLAGCRVVGSVLPQHLYGLTFRVFLPMALGLPLHAAMLWYVEQFAALSH
                     QHRYIFISSPAFLKRLDTQLSPPPVQVLLSAGGELPWQDVQHTASWLRVWPDEIYGST
                     ETGVIAWRYREQEQRRWLPLPGMQFQAEGDAFRLFSPLIAEDNGMLLDDILQFSEDGQ
                     FHLMGRRGRIVKIEEKRISLQEVEQRLLALDGIREAAAVPVTRGGRQSIGVLLVLDDE
                     AHLQWQNGGGHSQEMTWRRLLRPTLEPVAIPRYWRVIDEMPVNSMNKRVYAQLQELFH
                     EAP"
     gene            complement(30849..31430)
                     /locus_tag="ACHOUK_001299"
     CDS             complement(30849..31430)
                     /locus_tag="ACHOUK_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9896776.1"
                     /translation="MSGIRSLPMIKLLTGLLLLAWPFVIWFGLAHNGLHWLLPLMALL
                     LLLRLRQTRRQAGPLQAVTQLVAVVGIALCVASFMLKTHQLLLFYPVVVNAVMLAVFG
                     GSLWSAMPIVERLARLQEPDLPEKGVRYTRHVTQIWCGFFIINGGIALFTALYADMSL
                     WTAWNGMIAYLLMGTLMAGEWLLRRQVMKRDRA"
     gene            complement(31435..31686)
                     /locus_tag="ACHOUK_001300"
     CDS             complement(31435..31686)
                     /locus_tag="ACHOUK_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFP9896777.1"
                     /translation="MTEQQTVYQEVSALLVKLFEIDPQDIKPETRLYEDLELDSIDAV
                     DMIVHLQKKTGKKIKPEEFKAVRTVQDVVEAVERLLQEA"
     gene            complement(31698..31955)
                     /locus_tag="ACHOUK_001301"
     CDS             complement(31698..31955)
                     /locus_tag="ACHOUK_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HFP9896778.1"
                     /translation="MQALYLEIKNLIISTLNLDELTPDDIDTDAPLFGDGLGLDSIDA
                     LELGLAVKNEYGIVLSAESEEMRQHFFSVATLASFIAAQRA"
     gene            complement(31930..32751)
                     /locus_tag="ACHOUK_001302"
     CDS             complement(31930..32751)
                     /locus_tag="ACHOUK_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFP9896779.1"
                     /translation="MSKLMSRLEWTWRLVMTGLCFALFGLGGLLLSVVWFNILLVLVW
                     DTSRRRRLARRSIAASFRLFLTVAKGLGVLDYRIDGAEILHQERGCLVVANHPTLIDY
                     VLLASVMPETDCLVKSALLKNPFLGGVVRATDYLINSEAETLLPRCQQRLAQGDTILI
                     FPEGTRTKPGEKMTLQRGAANIAVRCASDLRIVTIGCSEHLLDKQSKWYDVPPDRPFF
                     TVAVHGRVEINKFYDATSQEPALAARQLNRHLLLQLQQGCLPLSGINNASALSGN"
     gene            complement(32748..33470)
                     /locus_tag="ACHOUK_001303"
     CDS             complement(32748..33470)
                     /locus_tag="ACHOUK_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HFP9896780.1"
                     /translation="MKFALNILDWQARAPGLSETLQWQEWSRQPQAIDPTAPLAKLSE
                     LPMMTARRLSSGSKLAVECGLAMLRRHQPDAVLYTSRHGELERNYRIVHALATEQALS
                     PTDFALSVHNSSVGNLTIAAKQPSVSSSLSAGRDSFQQGLCEVLSLLQAGYQRVLMVD
                     FDGFLPEFYHPQLPSEMPTWPYAVALVIEAGDDWQCETQSAIAGNETTLPQSMLFLQH
                     YLQNVDTFSLPGERVQWRWSHR"
     gene            complement(33511..34569)
                     /locus_tag="ACHOUK_001304"
     CDS             complement(33511..34569)
                     /locus_tag="ACHOUK_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFP9896781.1"
                     /translation="MYEQDSLSALDAITEAQRIAFAPMLFQTALCLRNAGVLSYLDRQ
                     GKHGATLADITEHSHINEYATSVLLDMGLSGRIITCKEGIYYLAKIGHYLLHDTMTRV
                     NMDFTQDVCYQGLFFLADSLNEGKPSGLKVFGDWSTIYPALSQLPDAARESWFAFDHY
                     YSDGAFNAALPYVFANNPTTLYDVGGNTGKWALRCCKYNENIAVTLLDLPQQIVLAKE
                     NIANAGFSDRIDFHAVDMLSDAPLPGEADIWWMSQFLDCFSPEQIISILSKIASVMKP
                     GAKVCIMELFWDAQRFEAASFSLNASSLYFTCMANGNSRFYSAEKFYDYLNKAGFQVA
                     ERHDNLGVGHTLLICQKK"
     gene            complement(34638..35015)
                     /locus_tag="ACHOUK_001305"
     CDS             complement(34638..35015)
                     /locus_tag="ACHOUK_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP9896782.1"
                     /translation="MKWFAAIAVVGALAGCARTAPIDQVHSTVSAGHTQEQVKKAILK
                     AGVERKWIMSEAGQGIIKARQQSRDHSAEIRINYTASSYDINYENSQNLQASGGQIHK
                     NYNRWVRNLDKDIQLNLSAGADL"
     gene            complement(35407..36456)
                     /gene="yhhT"
                     /locus_tag="ACHOUK_001306"
     CDS             complement(35407..36456)
                     /gene="yhhT"
                     /locus_tag="ACHOUK_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP9896783.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            36588..37805
                     /gene="yhhS"
                     /locus_tag="ACHOUK_001307"
     CDS             36588..37805
                     /gene="yhhS"
                     /locus_tag="ACHOUK_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9896784.1"
                     /translation="MPEPVAEPALNGLRLNLRILSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDVKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(37809..38366)
                     /gene="dcrB"
                     /locus_tag="ACHOUK_001308"
     CDS             complement(37809..38366)
                     /gene="dcrB"
                     /locus_tag="ACHOUK_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFP9896785.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(38439..39104)
                     /gene="yhhQ"
                     /locus_tag="ACHOUK_001309"
     CDS             complement(38439..39104)
                     /gene="yhhQ"
                     /locus_tag="ACHOUK_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFP9896786.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            39325..39570
                     /gene="tusA"
                     /locus_tag="ACHOUK_001310"
     CDS             39325..39570
                     /gene="tusA"
                     /locus_tag="ACHOUK_001310"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFP9896787.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(39672..41870)
                     /gene="zntA"
                     /locus_tag="ACHOUK_001311"
     CDS             complement(39672..41870)
                     /gene="zntA"
                     /locus_tag="ACHOUK_001311"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFP9896788.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFATSWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAALAIPAAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41944..42570)
                     /gene="yhhN"
                     /locus_tag="ACHOUK_001312"
     CDS             complement(41944..42570)
                     /gene="yhhN"
                     /locus_tag="ACHOUK_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFP9896789.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            42711..43070
                     /gene="yhhM"
                     /locus_tag="ACHOUK_001313"
     CDS             42711..43070
                     /gene="yhhM"
                     /locus_tag="ACHOUK_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFP9896790.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVL
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(43073..43342)
                     /gene="yhhL"
                     /locus_tag="ACHOUK_001314"
     CDS             complement(43073..43342)
                     /gene="yhhL"
                     /locus_tag="ACHOUK_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFP9896791.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(43332..43928)
                     /gene="rsmD"
                     /locus_tag="ACHOUK_001315"
     CDS             complement(43332..43928)
                     /gene="rsmD"
                     /locus_tag="ACHOUK_001315"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFP9896792.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADETLIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            44078..45574
                     /gene="ftsY"
                     /locus_tag="ACHOUK_001316"
     CDS             44078..45574
                     /gene="ftsY"
                     /locus_tag="ACHOUK_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFP9896793.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQTVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAENEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            45577..46245
                     /gene="ftsE"
                     /locus_tag="ACHOUK_001317"
     CDS             45577..46245
                     /gene="ftsE"
                     /locus_tag="ACHOUK_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFP9896794.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            46238..47296
                     /gene="ftsX"
                     /locus_tag="ACHOUK_001318"
     CDS             46238..47296
                     /gene="ftsX"
                     /locus_tag="ACHOUK_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFP9896795.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            47541..48395
                     /gene="rpoH"
                     /locus_tag="ACHOUK_001319"
     CDS             47541..48395
                     /gene="rpoH"
                     /locus_tag="ACHOUK_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFP9896796.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            48666..49769
                     /gene="livJ"
                     /locus_tag="ACHOUK_001320"
     CDS             48666..49769
                     /gene="livJ"
                     /locus_tag="ACHOUK_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFP9896797.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            49919..50185
                     /gene="ataR"
                     /locus_tag="ACHOUK_001321"
     CDS             49919..50185
                     /gene="ataR"
                     /locus_tag="ACHOUK_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin AtaR"
                     /protein_id="HFP9896798.1"
                     /translation="MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRA
                     AEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKRAAKRLQGM"
     gene            50192..50719
                     /gene="ataT"
                     /locus_tag="ACHOUK_001322"
     CDS             50192..50719
                     /gene="ataT"
                     /locus_tag="ACHOUK_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     acetyltransferase AtaT"
                     /protein_id="HFP9896799.1"
                     /translation="MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKIL
                     RAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQKKIPYKNIPSVTLGRLAIDRSL
                     QGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
                     FFPTKSIELLFTQSD"
     gene            complement(50716..51099)
                     /gene="panM"
                     /locus_tag="ACHOUK_001323"
     CDS             complement(50716..51099)
                     /gene="panM"
                     /locus_tag="ACHOUK_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFP9896800.1"
                     /translation="MKLTIIRLEKFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT        12314 a        13325 c        13818 g        11835 t
ORIGIN      
        1 taaataacac tactgatttc agaccagatc ctgaagatat cttcaaatgt ggacagacgc
       61 tcgaatgtgg cgacccccgc aaaaacatta gacaaaaata tggccatcat gggttagggg
      121 taaatcagaa tcaacaattt gataatggtg ttatggggct aaattccagg atggcagtgt
      181 ttgatgaaaa taaaaaaata ataggttctt cctcaggact aagttgtttt caaccacatt
      241 aaaactctat gagtgctgtg gtatatcttt ttaatcctgc cagtatttca tagtttttct
      301 taatcgattg tagaggcatg tactgatgac gcctctacaa tctttaggga ctttctctac
      361 tatgagataa gaaatttcat attatgcttt aaggctgaat gatttgtgca aaatataatt
      421 atttttccat cgaacatgag gtgtatgtca taagattaaa tgataactat gtattcctcg
      481 cggtgaatta tatgaggtaa aagatgaaaa ttggaactgt ggcaggtact aacgacagta
      541 caacgacaat cgcaacaaat gatatggtgc aagagcatgt tactaacttc actaaagaac
      601 tgtttgggta tattgcaaat ggtataggtg atgatatatc aagtattgcc aggacaatgc
      661 tcggtgaggt tctggaaaaa gtagatgatt ggcaaataga acggttccaa caatctattc
      721 aagatgacaa aatttcattt accatacaaa cggattattc agaaaaatat agtatgctat
      781 cgggcatgcg tgctcatatt ctacgtagga ataaccatta tcagtttatt gtgacgatta
      841 atagcaaaaa ttacggttgc cctcttgata ataccgatat taattggtgc ggcattgttt
      901 atttacttaa taatatgaca gtaaatgata atgctaatga tgttgcagtt actgagtcgt
      961 acaaacccat ctggaactgg aaaatatcgc aatacaatgt atctgatatt aaattcgaaa
     1021 caattataaa accccagttc gctgacagaa tctatttctc caattgttca cctgttgatc
     1081 ccacaagtac cagaccaacc tattttggtg ataccgatgg ctcagttggt gcagttttgt
     1141 acgcgctttt tgcaacggga catttgggaa tcatggctga aggagaaaat ttccttagcc
     1201 agttgcttaa tatagaagat gaagttctca atgtattgct aagggagaac tttaatgagc
     1261 aagttgatac taatgttaat acaataatca gtatcctgaa cagaagagac attattttag
     1321 aaagtcttca gccttattta gttattaata aagacgcagt gacgccatgt acgtttttgg
     1381 gtgaccaaac cggcgatcga tttagtaata tctgtggcga tcaatttatc atcgatttgt
     1441 taaaacgcat aatgagcatc aatgataacg ttcatgtatt agctggaaat catgaaacga
     1501 attgcaatgg taactatatg cagaacttca cgcgtatgaa accacttgat gaggatacct
     1561 acgacgggat aaaagattat cctgtatgtt tctatgactc caaatacaaa ataatggcaa
     1621 atcatcatgg gataactttt gatgatcagc gaaaacgtta cattatagga ccgataacgg
     1681 tctctattga tgaaatgacc aatgctctcg atccggtcga attagcggca attattaata
     1741 aaaagcatca tactataatt aatagtaaaa agtttaaaac tagccgggct atctcttgtc
     1801 gatcctttaa tcgttatttt tctgtttcga cagattacag acctaaactt gaagcactat
     1861 tagcatgctc acaaatgtta ggtataaatc aagttgttgc acataacggt aatggtggaa
     1921 gagaatgcat aggtgaaacg gggacagttt taggtcttaa cgcccgtgac agtaaacata
     1981 cgggaagaat gttcagcatg cataattgcc aaattaaccc tagtgcgggg cctgagataa
     2041 ccactccctg gaagtcttac caacatgaaa aaaacagaaa tggacttatg cctttgatta
     2101 gaaggcgcac aatgttacag ctctgaacca tagacaacat gaatattaat gttactatct
     2161 ggttgagact gttgataaaa cgtaataagg aatgctctgt tcctgcaact gaacaggcgt
     2221 gaaacgctat ccaacaggat ggataccgtt ttgtcagcca gttttataga tcacacttat
     2281 gagtatgcgt gtttcattta cggccaggaa tgctgatgat gacaacatta ctacccgttt
     2341 tcactaagcc ttccccgttg gcgctcaatg ctctgcgcgc tggtcgtatt tgtcgtttcc
     2401 ttcttatccc ggatggaaga atccgctgaa cgtcgttcgg cggcagttgt aacacgcgtc
     2461 gtttgcttac agggattatg gcgggtcgat tatggataag agtaagcgcc atctggcgtg
     2521 gtggggtgtc gggttactgg cggtggctgc tatcgtagcg tggtggctgt tgcgcccggc
     2581 aggtgtgccg gaaggctttg ccgtcagcaa tgggcgcatt gaagcgacgg aagtggatat
     2641 cgccagcaaa attgccgggc gtatcgacac cattctggtg aaagaaggcc agtttgttcg
     2701 cgaaggcgaa gtgctggcga agatggatac tcgcgtgttg caggagcagc gactggaagc
     2761 catcgcgcaa atcaaagagg cacaaagcgc cgttgccgcc gcgcaggctt tgctggagca
     2821 acgacaaagc gaaactcgcg ccgcacagtc gctggttaat caacgccagg ccgaactgga
     2881 ctccgttgcc aaacgtcata cgcgttcccg ctcgctcgcc caacgagggg ctatttctgc
     2941 gcaacagctg gatgacgatc gcgccgccgc tgagagcgcc cgagctgcgc tggaatcagc
     3001 aaaagcccag gtttcggctt ctaaagccgc tatagaagcg gcacgcacca atatcattca
     3061 ggcgcaaacc cgcgtcgaag ccgcacaagc cactgaacgg cgcattgccg cagatatcga
     3121 tgacagcgaa ctgaaagccc cgcgtgacgg acgcgtgcag tatcgggttg ccgagccagg
     3181 ggaagtgctg gcggcaggcg gtcgggtgct gaatatggtc gatctcagcg acgtctatat
     3241 gaccttcttc ctgccaaccg aacaggcggg cacgctgaaa cttggcggtg aagcccggct
     3301 gatcctcgat gccgcgccag atctgcgtat tcctgcaacc atcagttttg tcgccagtgt
     3361 cgcccagttc acgccaaaaa ccgtcgaaac cagcgatgaa cggctgaaac tgatgttccg
     3421 cgtcaaagcg cgtatcccac cggaattact ccagcagcat ctggaatatg tcaaaacggg
     3481 cttgccgggc gtagcgtggg tgcgggtgaa tgaagaactt ccgtggcctg acgacctcgt
     3541 ggtgaggttg ccgcaatgac gcatctggaa ctggttcccg tcccgcctgt cgcgcaactg
     3601 gcgggcgtga gccagcatta tggaaaaacc gttgcgctga acaatatcac actcgatatt
     3661 ccggcccgct gtatggtcgg gctgattggt ccggatggcg tcgggaagtc gagtttattg
     3721 tcattgattt ccggtgctcg tgtcattgag cagggcaatg tgatcgtgct gggcggcgat
     3781 atgcgcgacc cgaagcatcg ccgcgacgtc tgcccgcgca tcgcctggat gccgcagggg
     3841 ctgggcaaaa acctctacca caccttgtcg gtgtatgaaa acgtcgattt tttcgctcgc
     3901 ctgttcggtc acgacaaagc ggagcgggaa gtgcgaatca atgagctgct gaccagcacc
     3961 gggttagcac cgtttcgcga tcgtccggca gggaaactct ccggcgggat gaagcaaaaa
     4021 ctggggctgt gctgtgcgtt aatccacgac ccggaactgt tgattcttga tgagcctacg
     4081 acgggggttg acccgctctc ccgcgcccag ttctgggatc tgatcgacag tattcgccag
     4141 cggcagagca atatgagcgt tctggtcgcc acggcctata tggaagaggc ggaacgcttc
     4201 gactggttgg tggcgatgaa tgccggagaa gtgttggcaa ctggcagcgc cgaagagctg
     4261 cggcagcaaa cgcaaagcgc cacgctggaa gaagcattta taaatctgtt accgcaagcg
     4321 caacgccagg cgcatcaggc ggtagtgatc ccaccatatc aacctgaaaa cgcagagatt
     4381 gccatcgaag cgcgcgatct gaccatgcgt tttggttcct tcgttgccgt tgatcacgtc
     4441 aatttccgca ttccacgcgg ggagattttt ggttttcttg gttcgaacgg ctgcggtaaa
     4501 tccaccacca tgaaaatgct taccggattg ctgcccgcca gcgaaggtga ggcgtggctg
     4561 ttcgggcaac cggttgatcc aaaagatatc gatactcgcc gtcgggtggg ctatatgtcg
     4621 caggcgtttt cgctctataa cgagctcacc gtgcggcaaa accttgagtt acatgcccgt
     4681 ttgtttcaca tcccggaagc ggaaattccc gcaagagtgg ctgaaatgag cgagcgtttt
     4741 aagctcaacg acgttgaaga tgttctgccg gagtcattgc cgctcggcat tcgccagcgg
     4801 ctttcgctgg cggtggcggt gattcatcgc ccggaaatgt taatcctcga tgaacctact
     4861 tctggcgtcg atccggtggc gagggatatg ttctggcagt tgatggtcga tctctcgcgc
     4921 caggacaaag tgactatctt catctccacc cactttatga acgaagcgga acgctgcgac
     4981 cgtatctctc tgatgcacgc cggaaaagtg ctcgccagcg gtacaccgca ggaactggtt
     5041 gagaaacgcg gagccgccag tctggaagag gcatttatcg cctatttgca ggaagcggca
     5101 ggacagagca acgaagccga agcgcctccc gtgatacacg acaccacaca cgcgccgcgc
     5161 cagggattta gcctgcgccg tctgtttagc tacagccgcc gcgaagcgct ggaactgcga
     5221 cgcgatccgg tacgttcgac gctggcgctg atgggaacag tgatcctgat gctgataatg
     5281 ggttacggca tcagtatgga tgtggaaaac ctgcgctttg cggtgcttga ccgcgaccag
     5341 accgtcagta gccaggcgtg gacgctcaat ctctccggtt cccgttactt tatcgaacag
     5401 ccgctgctta ccagttatga cgagcttgat cgtcggatgc gtgcgggcga tatcacggtg
     5461 gcaattgaga tcccgcccaa tttcgggcgc gatatcgcgc gtggtacgcc tgtggaactc
     5521 ggtgtctgga tcgacggtgc gatgccgagc cgcgccgaaa ctgtaaaagg ttacgtgcag
     5581 gccatgcacc agagctggct acaggatgtg gcgagccgac aatccacacc ctccagccaa
     5641 agcgggctga tgaatattga gacgcgttat cgctataacc cggacgtaaa aagcctgcca
     5701 gcgattgttc cggcggtgat cccgcttctg ctgatgatga tcccgtcaat gctaagcgcc
     5761 cttagcgtgg tgcgggaaaa agagctaggg tcgattatca acctttacgt gacccccacc
     5821 acccgtagcg aatttttgct tggcaaacag ctgccgtaca tcgcgctggg gatgctgaac
     5881 tttttcctgc tctgcggcct gtcggtgttt gtgtttggcg taccgcataa aggcagtttc
     5941 ctgacgctca ccctggcggc gctgctgtat atcatcatcg ccaccggaat ggggctgctg
     6001 atctccacct ttatgaaaag ccagattgcc gccattttcg gtacggcgat tatcacgttg
     6061 atcccggcga cacagttttc cgggatgatc gatccggtag cttcgctgga agggccggga
     6121 cgttggatcg gcgaggttta cccgaccagt cattttctga ctatcgcccg cgggacgttc
     6181 tcgaaagcgc tggatctgac tgatttgtgg caacttttta tcccgttgct gatagccata
     6241 ccgctggtga tgggcttaag catcctgctg ctgaaaaaac aggagggatg atgcgccatt
     6301 tacgcaatat ttttaatctg ggtatcaaag agttgcgcag tctactcggt gataaagcga
     6361 tgctgacatt gattgtcttc tcgtttacgg tgtcggtgta ttcgtcagcg accgtcacgc
     6421 caggatcgtt gaacctggca ccgatcgcca ttgccgatat ggatcaatcg cagttatcga
     6481 accggatcgt taacagcttc tatcgcccgt ggtttttgcc accggagatg atcaccgccg
     6541 atgagatgga tgccggactg gacgccgggc gttatacctt tgcgataaat attccgccta
     6601 attttcagcg tgatgtcctc gccggacgcc agccagatat tcaggtgaac gtcgatgcca
     6661 cgcgcatgag ccaggcattt accggcaatg ggtatatcca gaatattatc aacggtgaag
     6721 tgaacagctt tgtcgcgcgc taccgtgata acagcgaacc gttggtatcg ctggaaaccc
     6781 ggatgcgctt taacccgaac cttgatcccg cgtggtttgg cggggtgatg gcgatcatca
     6841 acaacattac catgctggcg attgtattga ccggatcggc gctgatccgc gagcgtgaac
     6901 acggcacggt ggaacactta ctggtgatgc cgataacgcc gtttgagatc atgatggcga
     6961 agatctggtc gatggggttg gtggtgctgg tggtatcggg attatcactg gtgctgatgg
     7021 taaaaggcgt gttgggcgta ccgattgaag gctcgatccc gctgtttatg ctgggcgtgg
     7081 cgcttagtct gtttgccacc acgtcaatcg gcatttttat ggggacaata gcgcgttcaa
     7141 tgccgcaact ggggctgctg gtgattctgg tgctgctgcc gctgcaaatg ctttccggtg
     7201 gttccacgcc gcgcgaaagt atgccgcaga tggtgcagga cattatgctg atcatgccga
     7261 cgacacactt tgttagcctc gcgcaggcca ttctctaccg tggtgccgga ttcgaaatcg
     7321 tctggccgca gttcctgacg ctgatggcaa ttggcggcgc gtttttcacc attgcgctgc
     7381 tgcgattcag gaagacgatt gggacaatgg cgtaaggaag ggaaacttga cttcatactg
     7441 tgatgtcata ctattgatgt cataaagtga agtcataagg acatgccatg agaaccaagg
     7501 tacaggcttt acggaagaaa caaaaaaata cattggatca aatatttaaa actcctgttc
     7561 ctcaagggat caagtggtct gatatagagt cactggttaa agcattaggc ggagaaatta
     7621 aggaaggaag aggttcgcgt tgtaagttca tactaaatat gagcgttgcg tgtttccatc
     7681 ggcctcatcc gtcgccagat accgataaag gcgctgtaga aagcgtgcgt gactggttac
     7741 taagtatagg ggtaaaacca tgatcaaact gaagacgcca aactctatgg agattgccgg
     7801 gcaacctgct gttatcactt acgtgccaga actgaatgca tttcgtggca aattcctggg
     7861 cttgtcaggt tattgtgatt ttgtctctga cagcattcag gggctgcaaa aagaagggga
     7921 actttccctg cgagagtatc ttgaggattg taaagctgcg ggtattgagc cgtatgcgcg
     7981 tacagaaaag attaagacct ttacgttacg ctatccagaa tcgctgagtg aacgccttaa
     8041 taatgcggct gcgcagcaac aagtctctgt taatacctat attattgaaa cactgaatga
     8101 gcgtttgaat catttataac ttatagcggc ccaacaattt ctgttgcgcc gctatatttt
     8161 tgtggctctt aagatgcctt tctaaccaac cgatgcccct cattcatcat caggattttg
     8221 ctatacacat cttcctcgac ggcgtttttt gctgatgcca tgactcgtgc cagattcgcc
     8281 tcgttgtgat tattcacata cgctttgccg acggcggtga taaacgcttt gcgcagatcg
     8341 ctggcgatat ttactttagc gacgcgataa ttaacaaagc tgcgtaatat ctccggggcg
     8401 ataccggagc cgccgtgaat aaccaacggc accggacaga cttcggcaat gcgcttcagc
     8461 agggggatat cgatacgcgg tatatcgtcc aggccatgaa cgttgccaat agaaactgcc
     8521 agcatgtcgc aaccggtacg ctcgacaaag gtcttcacct tttccggttc cgttttgcaa
     8581 tcggcttcgc tgacgtgatc atcttctttg ccgagaatcg cccccagctc cgcttcaacc
     8641 ggtacgccat aggatttaca gaaatcgaca gcttcctggg tgaaagcgat attctcttca
     8701 aaagagaatg cggcaccgtc aatcatgacc gaggtaaagc ccgcgcgcac cgcctgttta
     8761 acatcctcct gggttttccc gtgatccaga tgaagactga ccggaacatc cattttatcg
     8821 gcgtgacgtt tcacgatttc ataaatgtaa tcgtatcccg agagttgtgc attggttggc
     8881 gcaatctgaa tgaagttggg taatcccgaa cgctcaatag cgtttaaaat tgaaattgtc
     8941 gtctccagat tggtggtatt aaatgcgccc gctaataccc gtttttcctg aatgcggtcg
     9001 agcagtatcc gtccattaac taatggcata ttgaaaacct catcttttaa tatcagttaa
     9061 ttattcagct tcaccgaagc ggtcgcgaaa taagtcggtg agagtctgta tggcctcgtt
     9121 ttcatcatca ccgtcgcagc gtatttccac cgtttttccg ccttttattc cggcgcgcat
     9181 aatattcatc atgctggtgg cattaatatc attaccctca tagtgcagcg tcactttaca
     9241 gctatatttt ttggcggctt tcgccagaca ggccgccgga cgtgcatgca ggccagtact
     9301 gtttctgacc tccacggttt tattcaacat agtgttcctc tcgatgatgt aatggcatca
     9361 cgccttcttg ttgtaatttc aactgatacc agcttttcca tttgtgctga taacgctgta
     9421 attgatggta tctcgcccga ttcggtaaca aacgttgggc tatagcgact ggcggcggtg
     9481 gaatatccgg tgcggcggtt aataatgctc cgacagaaac cgcctcgtta aatgcaattg
     9541 cttcaaccgg acgctgtaaa atatccgcct ttaattgcag ccaaagagga ttatgggttg
     9601 ccggaccgat cacttttaac gcgctggtgc caggcacgtt aaaacaggcg aagcagttgg
     9661 caaattccat ggttaatccc agtgatacgc tggcaattaa catgtcgata cttaacgtat
     9721 cgccaagccc ataaaataat ccacgggtat ggcgattttt atagggcgaa cccgaaccgc
     9781 gaagatgtgg aataaagacg ggaatgtcat cgagcaacca attccccgcc agataatcag
     9841 catgtccgcg agtaagtgat gcggcgatct cctcatcggt tagccggaac gtgttgcgca
     9901 gccactccag cgcgaaaccg cccacgggca gcgacgcaaa cagggtgaac tggttggcaa
     9961 gtgagtaaca gccgtttgcc agcttgtcac gtttggcctg ttcatccagc gtcggttgtg
    10021 tatccagttg cagaatgcct tccgtggtcc ccgtcgagtt aaggatatcg cctggcatca
    10081 tctgcaacgc tcgcgcaccc accatatggt catgtccggc cagcgtcacg ctgaccggtt
    10141 gcgagaaccc aagcgtcttg caaagtgact cgctgaccca tccggcgtgc tcgcctggct
    10201 gcaccaatgg cgcaaatgcc gtacagggaa cgtgtaacaa cgccgccgct ttcgctgacc
    10261 attcgccttt gcgcacgtcc agacacagcg tgcggctggc taaggtgata tccgtgtgct
    10321 gttcgccagt cagccgccag agcagcactt ccggcgcatg tagccagcgc aggccgcggc
    10381 gattacgcag cgggtaatgt tccagcaacc agcgcatttt gaaggcagag taattgctgt
    10441 gtagtggtag accgcagatg tcataaagtg ccgcactgtc tgcctcgcta agcgttgcca
    10501 gatactcttc accgcgacgg tcataccatg ccagcattgg cgtcaggatc tcgccatgct
    10561 cgtcaaggaa cacgcctgat tcgccaaaac tggcaatgct gatccgtctg actggcagcg
    10621 gcgaagcggc gttcagttgc gctatcgcct ggcggacctc ctgccagagg gcgtcgatat
    10681 cgaaatcgac atcgccctgt ggggagatct gctttgccgt cacgaattta tgcgcgccca
    10741 acgtcgttgc gtccaggcag gaaaagcagg tgactttgca attggtggtg ccaatatcga
    10801 taacgatagc tgcgctgtta tcaggcataa accgtgctcc gcgtcattcg gaaaatgcca
    10861 tacagtacca gggcgataac cggcaccaga ccaatggcga tgagattgcc gttgaagatt
    10921 tccagcagcc agaagcggaa cgggttaccg ccatcaagaa aactggaaac ctggccgctg
    10981 gagccttcgg caaatttgaa gttaacgtct ttcgccattc cggtaataaa cggcgcgatt
    11041 ttggtagcaa tccataggtc ggcaataatc accgggatcg ccgccagaac ggtgcggaaa
    11101 atattgccac ggctggctaa agcaatcatc gacgccatta ccgccaggtt ggcaaggtcg
    11161 ccaagcggca ggacttcatt acccggaagg acaaacgcga taaagaccga gattggcgtt
    11221 agcagcaggg cggtggagat aatcgccgga ttacctacgg caacggcgat atccaggccg
    11281 atatagagat cgtcacggtc agggtatttg gcattgagat actttttaat agcttcagac
    11341 agcggtaata aaccttccat caggatgcgt accatgcgtg gcaggatgaa catcacaccg
    11401 ccaatgctga tgccaagcag caatactttt ttgaaatcgt atccggcaat tacgccgagc
    11461 agaatgccca gaatagtgcc aaccatcatc ggttcgccga agatgccaaa ctttttctgt
    11521 acggtttccg ggtcgatatg aatacgattg aggccaggga tatggtcgat gattttgtcg
    11581 accagcagac cgaccgggaa gaacaccacc gaagagagcg tcggcaatga gatcccttcc
    11641 agtccaaagt atttttgtac cagtggcgca gaccagtcgg cgagtttaag tactaccacc
    11701 gcagcaatcg ccgccgccag caaaccaaag aacaggctgc cggtgctgta ataaaccaac
    11761 gtaccagcaa gggcaaaatg ccagtagttc cacatatcta cgtcaacggt acgggtttta
    11821 tttaacgcca gcatcgccac gttaatcaga atggtcaggg gaataacaaa cggggcaatt
    11881 ggcgaacccc atgtaatagc ggaaagcggc ggccagccta aatctaccac cggtaaatta
    11941 attccggtgc gttcaaccat cgcctgggcc gctggcccga cattgctggt cagaatatcg
    12001 aaaatggcat aaataccgac aaaaccaatg ccgactgtta atgacgaacg taaggcttta
    12061 gcgggtttta ctttaaaaca gagagcaaga ataaataaca ccaacggcaa cattaccgtc
    12121 gggccaaatc ccaatatata ttgcacaata ttatagaggg tatgcgcgat atcgttcata
    12181 ataaacctct ttttcaggcg agagcgcacc tcgcgttagc gcctgatgga gtgggttgaa
    12241 attaattatt attaattact ttttaaggat agaaacaatt tgcgccagta ctttttcttc
    12301 gccaataccg gtaataatcg gcaagccgtt caccaccggg acgtcgagtt cataaggcac
    12361 tttagttgtt gaaactacca gtgctacgcc gtcagtattt ccaggtaaag aggcaacgtt
    12421 ggtttgggaa taattgacgt taagaccttg ctctttgcaa tattccatga ctttttctgt
    12481 taccgccgtg gatgtggcaa tacccgtggc gcagacaaat aaaatggttt gactcatatt
    12541 tcacctcgtt ctttcatttt gtttaagatt actgtgtgta tttctgttgt ttccatcgca
    12601 agcagggctt gcataaaggg cgtatcctga atcatttcca taatccagcc cagggcattt
    12661 aattgtttat tgctttcgct taaggcgagc aaaaaaatca ccctgacagg tacggggtca
    12721 ggatcgccgc ccatatcggc aaactctacc ggttccggca ggatgccgac ggcaattgca
    12781 ttgtgcctga catgtttatg gtccgtgtgc ggaatagcga cgcacggtgg ttcggtaggc
    12841 agccccgttg ggtacgtcgc ctcacgtttc agtacgtgat cggcaaaatc agggacgacc
    12901 agctcggcgg caaccaacgg ttgcgctaac tggcggatga tctgttcaac gcttaaccct
    12961 gccggaagtc gttgaatata attttcctga aaatgtatgc cttgcatttt attcctctta
    13021 aaaaagtgta cttaactcac ttcccgccgt tgcagtacgg tgccaagata atatttattt
    13081 ccttttaacc agacgttgcc aaactcaatg ggtaatcctc gggagaaaaa gactaactgc
    13141 tccagatgca aaacgggtgc gtcttcactg atatctaaaa aatgaccacg ttcattacca
    13201 attaatcgcg cggcatagcg actttccgag taacgaattt ttctttgcga caaactttct
    13261 attgttggaa ataaattgtg ttgattaaaa tcgatttcag caataccggg gcatagctca
    13321 atattgatac ggttctctat cagcatcgct ttttcatcac caattgagcg taagcgttca
    13381 aggtaaagaa tatcctgacc gggcgttatt cttaatttct ctgccacata acgattagcc
    13441 gattcaatcc gtgacgtaat aacttcagtg gtaaaatgta ttttctggct ttccagcgat
    13501 tccgcgaatg acaataatcc ttcacctaac ggatatgcca cgttctcttt tttgacaaat
    13561 gttcccttac cctggacctg tattaacacg ccttctttta ccagttgggc gaccgctttt
    13621 ttaacggttc cccgactcac gtccagcata tccataatct ggtattccga aggaatgcga
    13681 tcgtcttcca ccagatctcc ggtataaata ctttctctta tccactccac cacttgttga
    13741 tagagcggta tttgtgacga cttacttaat gatttcatgg cgcgcacctg aacatttaca
    13801 gttgtctatg gacaatcatg gacaacttca agcgtatagt aggtgaacgg gagaaaaaga
    13861 ggatcgaagc gtctcacatt ttgagggacc aaatcgtgat caaaaaaatg tgacgtgagt
    13921 cgatatttgt cagaagttta ctgcgccgta aggaaataac ggcgcgttgg taggtgttaa
    13981 tcttccttcg gcaggcactg caaatgcccg tgccgcacgc cgcgctgggc gataacgtca
    14041 tcggcaaaat gctgcacgtc acccatgtca cctttcaaca cggcgatttc cagacagtcg
    14101 tcgtggttga tgtgcacatg tagcgtggcg acggagaggt cgtggtgatg atgctgggtg
    14161 gaaacaatac ggctggctaa gtcgcgtttt tcgtgttcat acacatacga cagcaccgcg
    14221 aaaccttgtg tgccgtgctg ctgggtagct tcctgcgcca gggcgctacg cagaatatcg
    14281 cggatagctt cggaacggtt gttataacca cgacgctggc tcaggctgtc cagcgtctcc
    14341 agtaaatcgt catcaagcgt gatggtgact cgttgcattt gcgttaaacc ttttctgtgg
    14401 cgcgacggcg cacggggaat gcgggtaata tcgcgttttg tagcacacgt ccggcgtcag
    14461 aggaaaaggt taatttctct cccaccgcct gggtttcgac gatttgtccg ttgtccatca
    14521 ccatcactcg ctggcaaaag cgttccacca ggcgtaagtc atgagtgatg aacaggcagg
    14581 cagtgccaaa ctgttgttgt agctttttca gcaggcgaat gacgcccgcc tgtaacacga
    14641 gatcaaggtt agaaacggct tcatccagaa tcagcaattt cggttcgacc gccagcgcgc
    14701 gtgccaggca gacgcgctgg agctggccgc cgcttaactg cggtgggcgc ttgtcgagca
    14761 cgctgtcgtc gagatcgacc gcgtgcagca tttcgctggc acgcgccagt tgttcggatt
    14821 ttttcagcga tagcaggtgg cgcatcggtt cccgcaggat ctcgcgcacg gttttgcgcg
    14881 gattcacggc gctgatggag tcctgaaata ccatctgaat atcgcggcgg aacgctttac
    14941 gctgggcgcg attgagcttt gccaacgatt cgccacgcca gctaatattt ccctgtgagg
    15001 gcgattctaa gcctaccagt agccgcgaga gagtgctttt tccgcagccg ctgcgtccca
    15061 gcagggcgac ggtttcaccg cttttcaggg tcagggaaac gttattcagc accgcctggt
    15121 gctgatgttt tccgctaaac ccaccgtggg catagtgatg ggaaaggtcg gagacgttaa
    15181 gtaaagtcat gatgccagct ccataccgta gagggcgaga tgagcggaaa ccaggctgcg
    15241 cgttaccgca tgtttggggg cgttaaacag cgtttcgaca tcgccctgtt cgacaatctt
    15301 accgtcagac attaccgcta cgtcatccgc cagacgcgcc accacgccca tatcatgggt
    15361 gaccagcagc atccccggcg cctgtttttg cataatgctt tccagcagat cgagaatgcg
    15421 tgcctgtgct accacgtcga ggtcggtggt tggttcatcg gcgatgataa acggtgattc
    15481 gcaaagcacc gccatcgcaa tcatcatgcg ctgcaacatg ccgccgctca tctcgaacgg
    15541 gtacagcttc agcacgcgtg cggcgttttc cagccccacc gcttctatgg cagcggtaag
    15601 cgtagcgtca tcggcgggtt tccccagcgc caggcacgtc tcccgcgcgt gggtgtgcat
    15661 ggtgtgcagt ggattaaagg cgctgcgcgg gttctgcatg atggtggcaa ttttgatgcc
    15721 gcgcagggcg cagggagaaa ccggtttgcc atcggctaaa atttcccccg ccgtctggcg
    15781 aacgcctgcg ggcagaattc ccagcgtcgc ggcgcaggtt aatgatttcc cgctgccgct
    15841 accgccgact aacgccagca cgcgcccgcg tttcagggtt aacgacacgc cgtgcaccag
    15901 cggctgcgcg gcctgtagcg cgatattacg tagttcaatc tgttgcggca ttagtgtgcg
    15961 tgctccgtca ccagatgagg atccagatga tcgcgcagtg cgtcacccac caggttaaag
    16021 gccatcacgc tgataaacag cgccagcccc ggccagaaca tttgcagcgg ctgggtccag
    16081 atatactggc gcgcgtcgtt aatcatcacg ccccattcgg cggtcggcgc ggtcacgccg
    16141 aggccgagga aggacatccc ggcaacgtgc agcatcatat ggccgatatc cagcgttgcc
    16201 agcaccagca gcgaagggat caccgcacct gccagatgat caacaaacac tcgcacatgg
    16261 cccgcgccag aaagccgtga tgccagcaca aactcgcgct ggcgcagtga aatcaccagg
    16321 ctgcgcacca tgcgtgcata ccacgcccag tgcgacaggg cgatggcgat aattacgttg
    16381 gtcagcccag tgccgagcac gccaaccata aagaatgaca gaatcgaggt cgggaaggtc
    16441 ataaacatat cggcgacgcg catggtagcc tgatcaacgc gcccgccaat cagcccggcg
    16501 ctgccgccaa taaccagccc taatgtcagc accagcagca ggcaggccat taccgaaccg
    16561 agcgacacac gggtcgctgc catcagccgc gagaaaatat cgcgccctaa gtgatcggtg
    16621 cccaaccagt gctgcgcatc cggcgaaagc aggcgcgacg gcaaatcaat cgcctggggg
    16681 tcatacggca gccaccactg gctggtgagc gcaatcagcg ccagcagggc gataatgatc
    16741 agcgccaggc gtaccgacca gcgggaagag aggaaaaagt tcacgcgtgc gctccttcat
    16801 gacgacgaat gcgtgggtcc agtgcggcgt tgagcaaatc gacaatcaaa ttacagacca
    16861 caaaaaccac caccatcatc agcgtaaagc actggatcac cggatagtca cggttaaaaa
    16921 tcgccgacac cgcatagcgc ccgacgccgg gccaggtaaa gatgttttcg ataatcatcg
    16981 tcccgccaat cagttcgcca atgtgcatcc ccacggcggt gatcatcggc agcgaggcat
    17041 tgcgcagaat atgacgacgc tcggtctgtt tgtcattcag gccgcgtagc cgcgcccagg
    17101 tgacgtgacg ctgaccggcg acgtccagca tactggcgcg cagcagccgt gcgttaatcg
    17161 ccagcgacat aaaggcaatg gaaaccgcag gcaaaatgat gtgctgccag ccgccgtagc
    17221 ccatcgcggg tagccattgc agatacaccg aaaacgccat caccagtaaa aatgccagcc
    17281 agaagttggg catcgacacg ccgagaaacg cgatgaaacg tacggcgaaa tccggcagac
    17341 ggtcacgatg gcgcgccgcc cagataccga gcggtacgga agtgagcaga attaatacca
    17401 gcgccgcacc cgccagttcc agcgtggcgg gcaggaagtt cagcatatcg tccagcaccg
    17461 ggcgctgcgt ggcaaatgag atgccgaaat caagatgcag cgccttccac aaccaggtgc
    17521 cgtactgcac atacagcggc tgatccagtc ccagcatggt gcgggtagag gccagcatct
    17581 ccggcgtcgg cggcaggtta gacagacgca aataatcgag cgccgggtcg ccggtaccga
    17641 ggcgcagcat cagaaaaatg atcaccgagg cggcgagcac catcgggatc agcagcagaa
    17701 agcggcgtaa tacgtaacgc aacattaagg tttcaccggt ttaatctgtt cgaacggaat
    17761 ttcggtggcg atcggcgcgt aggggatgtt acccagctcc ggttttgata ccaccatcat
    17821 tgagatgtaa ctgataggca gataaaccgc ctcgtcatgc agacgggtca gaatgtcgcg
    17881 atacagtgcc tgacgttgtg cttcgtcatg ggtcgccagc acttcgccga tctctttatc
    17941 aatcagcggc ttgtcggcta atccttgctg tgcctggaag tcagcgtgcg acggtacgcg
    18001 cattgaactc aggaaggcgt gtggatcgta tggcgcgccc caggtgcggt ggaaaatcat
    18061 gccaaaacga ccgtcgcgct gacgcgcata gatactgctc tcttcttcgc caatcagcga
    18121 gacatctgcg ccaatctggc gcatatcagc ctgaatgatt tccgccatcg atttgcttaa
    18181 cgcatcggtg ccgatgaacg aaagttcaat gtgcagcggc tgaccatttt tctcgcggat
    18241 gtctttgccc gcaggcagcg tccaaccggc tttttccagc aacgctttcg ctttctgcgg
    18301 atcgtactgg cgcggtttca ggccgaggtt ggcgtagggc acagaagggg caaacagggt
    18361 gtcggcgacc tgctgggtgc catacaacgc gttatcaatc agcgattttt tgtttaccgc
    18421 gtaattaaga gcttcacgta ctgccagctc attggtgggg gctttggcgg tattaagcgc
    18481 cagcatcacg gtttcgatcg gctgtgacag ttgggtatgg taagccgggt tctggctaaa
    18541 gcgggcgaag gtatcgagcg gtaataaccc ttcgtttccg tacagcaggt cgatatcgcc
    18601 agtttcaaac gccaccgcgc gggtagtcgg gtctgggatg acgttaaaag tgatcttctt
    18661 aattgccggc ttttcgcccc agtagttttc gttacggacg aagacatcgt actgattcag
    18721 tttcgattcc tgcaaaaccc acggtccggt gccaatcggc gctttaatcc cgttcatggt
    18781 ttcatggttt ttaaactgcg agggggcgat aaagcggaaa gggcgcggca gggccagttc
    18841 ttgcaggaaa ggatagtagg cgcttttcag ggtgatttgc agctctgttt tactgagtgc
    18901 tttaacatca acaatctggt ttgccagctc cagccaggcg tgacgttgac ggttatcgag
    18961 cactgcgcgg aagttttctg ccgccgcctc tgcatcaaac ggttcgccgt tagagaattt
    19021 cacgtcatca cgcagggtga aggtccaggt tttaccatct tctgaatgag tccagctttt
    19081 tgccagccac gggatcaccg aaccgtctgc ctgatatttc accaatggtt cataaaccat
    19141 gctctgggcg aacatctggt taggcgtgta aaggtgcggg tttagtggcc cgacattcac
    19201 cggccaggcg gtggtgattt catctggtgc agcggcatgg acgataaaag acgcacaagc
    19261 cagcagcgca aatagagtgc ggcggagtgt ggagagcatg gtaaccccaa tggattaaaa
    19321 tagatggcgg aaataagtat gacgatttta agtattcgtc atactgatta cctgttctgg
    19381 atcaatagat gggcagtcgg gtgggcggat cagttaactg aatcgatcca ttgcacactg
    19441 tcggcggtga gcgtaaaggg agtaggggtg cagatcgcca ggcttaaatt ttctagctga
    19501 caatggctga ctgacagcga ggagtgatag gtgctgtcta cgctgacgat ttgccaggcg
    19561 ctgccaccac gctgtttaac gatggcttct ttgcgcgtcc agatgcgcca gaacatttcc
    19621 agttgctgat caggatgcac ggcgtccatc tcagcatgtt ccccgaggct gaacacggcg
    19681 ttcgccagcc agcgccagtt ggcgcgcggg cgaatcactt cgatatcgca gccgacttcg
    19741 ccttcatcac tcaacagcag ggcgatatcg tcaccgctat ggcttaagtt gaaccatagc
    19801 ggcgtttccg gcgcaaatgc aggtttgcct tgttcgccat agatgatctc cggtagcggg
    19861 gaaagcgtgt gcgaaagcaa tgcacgcccc gccagccagc gttcgcgtcg tggaccttgc
    19921 ggtgcttgct cgcgtaaacc cggtggcagt ggagctgcgc ttaaggtcga aactttcccc
    19981 agaactatcc gatacatatc agggccaacg tttaatgatg atggatgtat taatgccgcc
    20041 aaaggcaaag ttgttactct gcaaaaactc acaatcaacc ttgcgggctt catgcatgat
    20101 gtaatccagc gcgccacaat tcgggtctgg tttattcagg tttagcgtag gcgcaaacca
    20161 accttcgcgc atcatttgca gactcatcca ggcttcgagc gcgccacagg caccaagcgt
    20221 atgaccaaaa taacttttca gcgaggagag cggcacatta tcaccgtaaa tggttgctgt
    20281 cgccagactt tctgccatat caccgcgatc ggtcgccgta ccatgcgctg aaatatagcc
    20341 gatatcttgt gcgcttaacc cggcgatttt cagcgattgc tccatacaat actgcatggt
    20401 ttcgcgctgc ggctgggtaa tatgtgccgc gtcgcagtta gtcgcaaagc cgacgatttc
    20461 accgtaaatg gtcgcgccgc gcgctttggc gtgctccagt tcttcgagaa tgagcgtacc
    20521 tgcgccttca ccaatcacca gcccgtcgcg gttttcatcg aacggtgaag gtgtggtttt
    20581 cggcgcatca ttgtgctggc tggtggcgaa aagcgtatca aataccgctg cttctgaagg
    20641 acataactct tcggcaccac cagcaaccat caccgtttga taaccgtggc gaatggcttc
    20701 ccacgcataa ccgattgcct ggctgccgga agtacaggcg ctggaggttg ggatcacccg
    20761 cccgcgcagg ccgaaaaaga ggccagtatt aacggcggtg gtgtgcggca tcatctgcac
    20821 ataggtggtg ccagtgatgt tattggtgtg cttttccgtc agcatggtgg cgaactcgct
    20881 taccgggccg gtactgccgg tcgatgaacc gtacgcaatg cccgtttcgc cgctggttaa
    20941 tatcggatcg ccaatcaacc ctgcctgctc taacgccagc tcgctggcac gggtcgacat
    21001 ttgcgacacg cggcccatcg cacggatacg cttgcgggtg tagtgctccg gtagcgtgaa
    21061 atcatcaaca ggcgcgccaa gcagggtgtg cagcccatcg tagacctgcc actccggcat
    21121 tttgcgcacc gcattttcat atgccagtaa gcgtgcagaa acgtcctgcc agttttcacc
    21181 aaaggcagtc acgccgccca ttcctgtaat cactacgcga cgtgtcataa catccctccg
    21241 ttgatggaaa taacctggcg ggtgacgtaa cccgcaatat cagacattaa atagctggca
    21301 agcccggcaa cttcttccgc ctggcccatg cgtttcatgg ggatcatcga cattgcctct
    21361 ttcagggcgc tctcttccat ttcgatcatc ccggtatcaa taagccccgg cgcgatgcag
    21421 ttgacggtga ttttgcgctt cgcgagttcg atagccagcg cttttgttgc gccgataatt
    21481 ccggctttgg cagcgctgta attcacctgg ccgcgattgc ccattacgcc ggaaaccgag
    21541 gatagcgtga tgatgcgccc accctggcgt gcgccgatca tcggcataat gcacggctga
    21601 atgacgttgt agaaactgtc gaggttggtg tggatcaccg catcccagtc gtcatcgctt
    21661 aaagccggaa aagcggcatc gcgggcgatc ccggcgttac tgaccacgcc gtaccaggca
    21721 ccgtgttgcg cgatttcgtg ctccagcact tcccgacact gttcgcggtt tgcgacgtcg
    21781 aaactgagca aacgcccgtt acccccattg gcgacaatgg cattcagcgt ttcctgagcg
    21841 cctgtggcgt cacggtgata gtgaacgcca atgttaaagc cgtctgccgc cagttggcag
    21901 gcgatggcgc gaccaatgcc tttgctggca ccagtaacca gaactgaacg actcatcagg
    21961 acgctccttg ttgaaacagg gtggtaagtt cttccgcggt cggctggaag gtgttaatgc
    22021 ggcccgtcgc ctcgccgtcg ttgatgctgc actcaaaact gccgaagcga tcgtcttgca
    22081 tcagcagttt gacggtaatg gtcagtgtct ggcctgcggg cagaatgcca gtggtgcaaa
    22141 gcaattcacg cgcgcccagt atcatcccta actcaatact gtttttgccc tgctggtggc
    22201 gatgccagcc agaccagacg ccgacggttt gcgccatcag ttcaagggta aaccagccgg
    22261 ggagattgcc gtccgggtcg agaaacggtg ccagtacgcc actgggcgaa accgtcacgc
    22321 ggcacaccgc gctgtcatcg ctgacgctca ccacgtcttc cagcagtaac atgggcgcgt
    22381 cgtggggcag ataagcgccg ggggataaat agtggctcac gaaaccctcc cgagcagaat
    22441 gctggcgtta ttgccgccaa aggcgaagga attggacagg atcaccggac gtgccagcgg
    22501 ctgcggtttt tcgataatgc cacacggtgg cagcgtggga tcgcgcggcg acaggctgaa
    22561 gtcctgagcg ggcagcggta aatcacgctg taaaatcaac atactaattg ccgcctcggt
    22621 aatgcccgct gtgcccagcg tgtgtccggt caaatgtttg gtggagctgc acggtacgcg
    22681 ttcgccaaac agggcgttaa ccaccattga ttcaatctga tcgttgagtt gtgtggcggt
    22741 gccgtgcagg ttgatatagc cgacgtcatc tggcgtaagc tgcgcatcgg tcagcgcctg
    22801 attaatagcg cggatcgctc cttcgccctg cggatgcggg gctgaaatat ggtaagcgtc
    22861 gctcgattca ccaacgccca gcaacgcgat gggctgcggt tcccgggtca gcagcattag
    22921 ccccgcccct tcaccaatgg tgatacctgc gcggtcacga ccaaacggct gacataaggt
    22981 tggcgacagc gattcgaggc tgtgaaaacc gttaatcggc atccggctca gggtgtctgc
    23041 gccacccaca atggcggcat cgaccagccc cgcttcgata agcctgcgcc cgctgattat
    23101 cgctctggcg ctggaagagc aggcggttga aagggtatag gccgggccgt cgagcgccag
    23161 ccagtggctg agaaaacgcg acggatcgcc gagttcctgt tgcgcgtagt gccatgtggt
    23221 gctcggctgc ccgtgggtgc gcaggttaac gtgcgtgtcg ccttcatgca ggccggaggt
    23281 gctggtacca agcacaatcg caatgcgctc gcggccatat ttcgcaatgg cgtcatcaac
    23341 ctgcggctgg atctgcgcca gcgccgccag caggatctga ttgttgcgcg agcggtgcgc
    23401 agcgaatttt tccgggataa gcggaagctc gccatccacg ccagccagga ccgcctgcgg
    23461 atgcccctgc aaccagcctg cgcgcgggcg catgccggga gcaacaccgc gagtcaggtt
    23521 ggttgcgatt tcgtcaaggt tatttcccag ggcgttgatc atgccgacgg cggaaatata
    23581 aatcatatca gtcacctaag tattgaatgg tgatgtggta tttaaagaca tgctgctcaa
    23641 tgctgattgg cacgcgtttg cccttgcgat tcagataggt gatttccgtg accagtttgc
    23701 cgctggcgtt acgcagctcg cgtttgtcac cgttgtcgcg aagtgtccat cctgcgggaa
    23761 gttgcggttg ccaggcgcta atcggccagt ggctgagcat cacgtcagcc agtacctgac
    23821 ttgcgggcgg taactgtggg acgacgatgg attgctcggc gcgtagccct tgcgcatcgt
    23881 aggtcaccag aaacaggcga atgccgaccg acgacagccc ggcaagggtg attttctgat
    23941 cgtcggcgtt aagcatcact aacagcgact gggttttgcc gttgaagctg ccggtcaaca
    24001 gttgctgcga attgactgcg ggtgaaatcc ctggtgccgg cagcgtgatg cgcgtgccag
    24061 gttgcagcca cgcctgcggg cggccttcct gttctggttg cgagtggcta cagccggtga
    24121 ggataagcgt agcggtcagg gcgaacgctc gccagaatgt ggatttgatc atttttttgt
    24181 tctcttttta tcgggcatag ccagcggcga aagcaggaag gcggtgaaaa taccgctcac
    24241 cagcacaatg ccaaaactgc tgatggcctg ggtggcgctg aataccagca tacccagcgt
    24301 cagcaaggtg gtgagcattg ccagcgcgat cgccagtagc gaagttaacg gtgtaccgcg
    24361 cggattactg aaaaacagcg tgtagttgat accgatgcca agcaccagca ccagcgccag
    24421 cagcgaaaag agattcaccg cctgcccgct catcgccagc accgccagac cacagcccag
    24481 cgaaagcacc gaaggcacca ggctgataag ccctttgcgc cagccgagac gggccactgc
    24541 gccgcaggca atcactgcca gcgccaccag caacaagccg gttaagacgt agcggtaaag
    24601 tgcgaacaat tcatcaaagg tgcttttgcg atcaacccag gcaatgccgc aagggtaata
    24661 tgtggcgatt tcctgcatca acgcgctact tttaacccct tcaaccggca ccagtacgcc
    24721 gctttcgccg ttttccagcg tcagccacag cagacgccag ccttcactgg cggggcttgc
    24781 cagccattca tcaacgttca ctggcatggc gttgagatcc gggttcactg ccgtcagccc
    24841 ggcattttgc agcgcttttg ttactgtcgg ggccgccgtt ttcagcagtt gtaaatcttc
    24901 ctcctgccgc gcaagggagt tcagcggaat ggtgcggtag ttgctgataa gcccctcttt
    24961 tttcgcatac tcaagtgagg cggtatattt ctccagtcgc cgcaatgttt gctgtggcga
    25021 atcgccgtaa accacaaacc atttttgatc gacgctctgc ccggtcaggg cggtaatggc
    25081 tttttcctgc gccagaatat gctgcggtag cgcctgtaac tgcgagatat cgtcatcgac
    25141 gcgtagcatt gacatccccg ccagcgaaaa cagcgccagc gcgacgggca gacccagcga
    25201 cagtttttta ttacgccgcc acgctgccag ccagcgtagc atcagcgcca tcgccggaac
    25261 cggacgcacc ggcaggccac ggcacagcca cggatgccag aacaggacgg tcagacagga
    25321 ggcgctcaac ccgacggcgg caaaaatcgc catctggcga atgccgggga agggggcgag
    25381 catcataatc agatacgccg ccacggtggt gagcagcgcc agtagcaagg cattgcgtac
    25441 tttcgccagg ctttgccacg gcgaaacgtc gttgccgtga accatccgct cggtgagata
    25501 atagagcgtg tagtcagcgg aaatgccgat aacgctcatg ctcatcacca gcgtcatcag
    25561 gtgtaattca ccgaaaatca ataaagtggc gaccgttccc gccagcgcgc cgatgccgat
    25621 ggaaatcacg caaagcagca acgggcgtaa agagcggaac accgccacaa tcagcaatat
    25681 cacccccagc agcgtagcca cgcccagggt ggagatatcc tgcttcgcct gttggctggc
    25741 gtaatcgctg taaaacaccg tgccgcgcga gagcaactgt gcctgcgggt aacgcgtttt
    25801 cagatccttt tccagcgtat tcagggtcgt gatcagctgg tgggtttgct gcatatcaaa
    25861 cgacgatccc gccagttcgc cgtgcagcag ataccagtag ttgccctggg gatcctgcgt
    25921 caccagccaa ccgtccatca gccgcaaacg ctggccgttt ttcgccattg ccagctgcga
    25981 gccgcgcatt aacatcagcg gatcgttttg cagctccttg ccgcttacgc cggagaatgc
    26041 ggaataaagc tgggagagga tccactgtgc ctgcgcttcg ccgccgtttt gcaggcgggc
    26101 gcgggtgttg gggtcaatca ggccgttgcg atgctgccag aaaaacgctc cccacgcctg
    26161 ctggctggcg gcatccattg gccctttaac gtcgccgagc gcagcggatt tttgcagcag
    26221 cgtcagccac tcctgagcga ccaaaggatt agcctcttta ccggggctga ccagccacac
    26281 cagttggcgg tcaagacgct gcataaagcc gtcattcagc gccggaggaa tatcgcccat
    26341 cgtctgtttg ggtaacatag ccagcacgct actgttcagc cgtgattgcg gcagcaggat
    26401 cagcaacgcc accgccatga ccaggcagac tagcccccat aacagcgcgg ggcgtttact
    26461 gggcggcaaa acgttggcgt tcgtcatcgg tcagttgcgc tggcgtcagt tgatgttggg
    26521 taagagcaat atcggtgcga tcgccctgtt tatcattaag ttgaatgctc tccagatagg
    26581 ttttcccggc gagatcgatg gtgttgaaaa ttttatccag cggcgtggtg gtcggcgtca
    26641 ggcgcagcgt ccagcggcct tcgcctttgt cagcaaattc gacgcggaag ttttgttcca
    26701 gcactttgcg atcggcctgg aacaacgcgc gcagcaggtg gttaaactgg aacatctgcg
    26761 ggttgttttc tgccgtgatg atttgcggcg gctgaccgtt gatcacctgc accatgcgtt
    26821 tatcgtccag taatagttgc atggggaacg gtgaggtttg atcccacaac agcccctggt
    26881 cgcgggcgat caacatctgg ccctgagatc gcagcggctg cggcagatct ttaatcgtcc
    26941 gggtttgatc gaaatgggcg cggatcaccg gttgttcggt aaagcgttgc tgaagatcgt
    27001 ccagggtcag ggcgctcaca aacgggctaa tcagcagcgc cagcagcggt aaaaatttca
    27061 tggtttgact cccatgcgtt caaacagaat atcggggctg acaaagcaca gttcgcgact
    27121 ttgctcgtcg accgcgacct gaatggtgta tccggtggtg gcgcgtttgc ccgtttcggc
    27181 atcgaaaatc tgatagccaa tccgcagacg gttttcgaac tcttcgatat gcgcacgcac
    27241 gcggatgcgt tgctcaaagg tgagggcgtg gcgatatttc acccgcgcat cgaccactgg
    27301 ccacaaataa ccggactctt tcatttgccg atagccgtaa ttgaactggt tgagcagcgc
    27361 ctcgcgagcc acttcaaagt agcgaaaata gttaccgtgc caggccacgc ccatcatatc
    27421 gacgtcgtga aagggaatgg tcagctcgac ttctgcggta aatcggggat cgttcagcac
    27481 cctttactcc ttgtcctgaa tttccggcag ttgccagaaa tcgaaaaaat taaaccagtc
    27541 gagaggcgac tggagcgcgt aatgttccag acgcgcggcg taatgatcga tagcgttttg
    27601 cagcgcctgt tggcgttcgc cgcgcggcag ctgcagtggg tcggcaaagc tttcgcagtg
    27661 aatatgcagc ttgccgtgct ggcgcagggc gaaaatcaga ttcaccgggc agcgcaaaat
    27721 agaggcgaga ataaacggcc cctgtgggaa aggcgcaggc tggcccataa acgaactcca
    27781 gcagacgcgc cagtcgccgc cgcgttgcgg gttgacggcg atgcggtcac cgacaatcgc
    27841 cacccattcg ccgttatcca gcttctcttt cagcaggatg gcggtttctg ggccgatatc
    27901 tgttaccggc atcaggttaa tgcctgcctg aggagccatc tcctgcatta tctgtttaaa
    27961 gcgttgggcg ttttcgctaa acaccagcgc gttaatggtt ttgtagccct gaatttttgc
    28021 cagcgcccgg cacacttcca catcgccaag atgcgaggcc agcagcaatt tgccgcgcgg
    28081 atcgcggacg tcaagcgctg cttctgcgcc tggcgcaaac agcacatcac gccctggttg
    28141 tagctcgccg cgccagctgg cgattttgtc gagcatggca ttaccgaaac gtagaaagtg
    28201 ctgatagctg ttgagttttg ccgcctgtgg ttgatgctgt cgtacacggg cgagccagtc
    28261 ttgcgacgct ttgcgcgcac gagaagcagt gagccagtag acgcccacca ccgggtaaag
    28321 cagcgcgcta aacgccgttc ttcccagcag acgccagacc agcagcatca ggcgcattcc
    28381 ccataatcct ttcacttcac tctgccgcgc ccagtggcag gaagagcggc gcatcagcag
    28441 tgaaggaata cgcggcaaca tgccgaaaaa cagacgcgtg tgcatgagcg agatgcggac
    28501 gttatctttc agggcatcaa aatgcgaaag cccgtccagt ggataggtga cgcgggtcgg
    28561 cacgaaatag ctggtatttc cctgccagta gaggcgcacc atcacttcgg tgtcgaaatc
    28621 catccgcttg ccgatggtgg cgtgttttgc cagttgcagc gttggcgcaa ccggataaac
    28681 gcgaaaaccg cacatgctgt ctttcagttg cagggagagc gtttcgatcc atacccagac
    28741 gtgggtgacc cagcgcccgt aaagccgtga gcgggggatg gagtcatcgt aaattggctg
    28801 gccggagatc agtgccgcag gttgttgttc agcgagagcc agcagtttag ggatatcttc
    28861 aatcgcgtgc tgaccgtcag catccacctg caccgcatgg ctgaaccctg cgtctgccgc
    28921 tgcctgtaag ccacgcatca ccgccgcgcc tttgcctgcg ttttctgcca ggcgaattaa
    28981 ggtcacgcca ggctgttcgg caagcagact gtccagttgc tgttgtgtgg tggcatcgct
    29041 gccgtcatcc accacaatac agggcagatt aaatggctta agacgcgcca gcacgcccgg
    29101 catcatcgcg ccgtggttgt agcagggtat caacacgcag ggagaaaagt ttaccgacat
    29161 aggcggatct tcccgctact ggcggtgtgg cgggcgtcac cgtcgtgacg ctgatagcta
    29221 aagctcagaa tctggcgttc ttcctgccag ttaagcgcga gcgtgacgcg gttttccggc
    29281 agcaacggag actggaattt tatgttctga atggagtgaa aacgccagcc aggcgcgagc
    29341 agattgagcg cgtagctcat cgcccagtcg atttgcgcaa ctcctgggag taatggttgc
    29401 acggcaaaat gaccgcgaaa ccagaacaga tccgccctga gatgcagaac aatttcaagg
    29461 tgattcgttt gtgcctgatg gcgctcgatt tcatggggct tcatgaaata actcctgtaa
    29521 ttgcgcatag acacgcttgt tcatactgtt tactggcatt tcatcaataa cgcgccagta
    29581 acgcggaata gcaactggct ccagcgtcgg gcgcagtaaa cgccgccagg tcatttcctg
    29641 gctatgcccg ccgccgttct gccattgcag atgagcttcg tcatccagca ccagcaaaac
    29701 gcctatgctc tgacgcccgc cgcgcgtaac gggaactgcc gccgcctcgc gaatgccgtc
    29761 cagcgccagt agacgttgtt ctacttcctg gagtgaaata cgtttttctt caatcttaac
    29821 gatgcgccca cgacgcccca tcagatggaa ctgaccatct tcgctaaact gtaagatatc
    29881 gtcgagtaac atgccgttat cttccgctat cagcggtgaa aagaggcgaa aggcatcgcc
    29941 ttcggcctgg aactgcatac ctgggagggg tagccagcgg cgttgctctt gttcgcggta
    30001 gcgccaggcg atgacgccag tttccgtgct gccgtagatt tcatcaggcc agacgcgcag
    30061 ccagctcgcg gtatgttgta catcctgcca cggcagctca ccgccagcag agagaagcac
    30121 ctgaacgggt ggcggagaaa gctgggtatc caggcgcttc aaaaatgccg ggctgctgat
    30181 gaagatatag cgatgctgat gactcaacgc ggcaaactgt tcgacatacc agagcatggc
    30241 ggcgtgcagc ggtaatccca acgccatggg caggaaaacg cgaaaagtca ggccgtacaa
    30301 atgctgcggc aatacggaac cgacgacacg acaacccgcc agacgtgcgc ctaatcgttc
    30361 agccagcagt tctgcttcac gatccagcaa atgaacaggt ttaatcgccc gtttcggctg
    30421 tcccgtggaa ccggaggtaa acagctcgat aaaagcctct ggcgctatag cggcaaaggt
    30481 gaaggattgt gtcgcgattt gtggagaact tgctaccaac agcaatgaac cttgccagtt
    30541 aagttcgctg tcgctaagca caccatcaaa aagttcacgt tgctcgttaa gctggataac
    30601 acggttatgc cccggaagca ccggcgtttt tccggcgtgt agcgtggcga gcagggcaac
    30661 gataaacaga tagctgttct caaagcacag cgcccagcgt tcacccggct gttgctgcaa
    30721 gcggcagata agttgcgcga catcgtgacg caaatcgccc agcgtccagg tactttcgtt
    30781 cagccacgca atgggtgtgt catcagggcg tggcgcagtc agccagcgtg caagcggtaa
    30841 ggtctgcttc atgcgcgatc tcttttcatc acctgacggc gcagcagcca ttcaccagcc
    30901 attaacgtcc ccatcaacaa ataagcaatc atgccgttcc aggcggtcca taacgacata
    30961 tcggcataaa gcgcggtaaa gagggcaatt ccaccgttaa tgatgaaaaa gccgcaccag
    31021 atttgcgtga cgtggcgggt atagcgcacg cccttttccg ggaggtctgg ctcctgcaac
    31081 cgcgccagcc gctcaactat cggcattgcc gaccacagcg aaccgccaaa aaccgccagc
    31141 atgaccgcgt taaccactac cgggtaaaac agcagtagct ggtgcgtttt caacataaag
    31201 ctggcgacac ataatgcgat gcccacgaca gcgacgagtt gcgtgacggc ttgtaaaggc
    31261 cccgcctggc gacgggtctg gcgcagacgc aacaaaagca gcaacgccat tagcggtaac
    31321 agccagtgca ggccgttatg cgctaagcca aaccaaatca caaacggcca ggcgagcagc
    31381 aataatccag tcagcagctt aatcatcggt agcgagcgaa tgcccgacat cagatcaggc
    31441 ttcttgtagc aggcgttcta cagcctcgac gacatcctgg actgtacgca ctgctttaaa
    31501 ctcttccggc ttgattttct taccggtttt cttttgcagg tgcacaatca tgtcaacggc
    31561 gtcgatgctg tccagttcca gatcttcgta caggcgcgtt tcaggtttga tgtcctgcgg
    31621 atcgatttca aacagcttaa ccagcagggc tgagacttcc tgatagacgg tttgttgttc
    31681 tgtcatgatg gctttcctca ggcacgttgc gcagcaatga aagaggccag agtggcgacg
    31741 gagaaaaagt gctgacgcat ctcttcgctt tccgcagaga gcacgatgcc gtactcattt
    31801 tttactgcca gtcccagttc cagggcatcg atagagtcca gccctaaacc gtcgccaaac
    31861 agcggcgcgt cggtatcgat atcatcaggc gtcaactcgt ccagattgag ggtgctgatg
    31921 atgaggtttt taatttccag ataaagcgct tgcattattt attcctgaga gaggtaaaca
    31981 accttgttgt aattgaagca aaagatgccg gtttaactgc cttgctgcca gcgccggttc
    32041 ttgtgaagtt gcatcgtaaa atttattgat ttcaacccga ccatggactg caaccgtaaa
    32101 aaacggccgg tcaggtggaa cgtcatacca tttactttgt ttatccagca gatgctcgct
    32161 acagccgatt gtcacgatgc gtaaatcgct ggcgcagcgc acggcaatat tcgccgcccc
    32221 gcgctgcagc gtcatttttt caccgggctt cgttcgtgtg ccttcgggga aaatcaggat
    32281 agtgtcaccc tgtgccagtc gctgttggca gcggggtaac agagtttcag cttcgctgtt
    32341 gattaaataa tctgtcgccc gcacgacgcc gccaagaaaa ggatttttta acagcgcgct
    32401 tttcaccagg cagtcagttt ctggcatgac tgacgccagc agcacgtaat cgatcagcgt
    32461 gggatgattg gctacgacga ggcaaccgcg ttcctgatgt aagatttcag cgccatcaat
    32521 acgataatcc agtacgccaa gccctttcgc gacagttaaa aacaggcgaa aactggcggc
    32581 aatgctgcga cgcgccaaac gacggcggcg ggaagtatcc cacaccaaaa ctaacaatat
    32641 gttaaaccac acgacagaaa gcagcaggcc gccgagacca aacagggcaa aacataaccc
    32701 ggtcatgacc agtcgccagg tccactccag acgtgacatc agcttactca tctgtggctc
    32761 cagcgccatt gcacgcgctc gccaggcagt gaaaatgtgt ctacattttg taaatagtgc
    32821 tgtaaaaaca acatgctttg cggcagtgtt gtttcattgc ccgcaatggc tgactgcgtt
    32881 tcacattgcc agtcatcgcc agcttcaatc accagcgcga cggcgtacgg ccaggtcggc
    32941 atttctgatg gcagttgtgg gtgataaaac tcgggcagga aaccgtcaaa atcgaccatc
    33001 agcacgcgct ggtatcccgc ttgtagcagg ctaagcactt cacacaaacc ttgctggaaa
    33061 ctatcgcgac cagccgaaag cgatgacgaa acgctcggct gtttggccgc aatggtcagg
    33121 ttacccacgg aagagttgtg tacggagagc gcgaagtccg tcggagaaag cgcctgttcg
    33181 gttgccagtg catgaacaat gcggtaattg cgctccagtt cgccatgacg actggtatac
    33241 aaaacggcgt ctggttgatg gcggcgtagc attgccagac cgcattcaac agccagtttg
    33301 ctcccggagc tgaggcggcg ggcggtcatc attggcagtt cacttaattt tgccaacggt
    33361 gctgtcgggt caattgcctg aggctgacgt gaccactcct gccattgcag cgtttcacta
    33421 agtccaggcg ctcttgcctg ccagtcgaga atgtttaatg caaatttcat caacgcgcgc
    33481 ccgcggtaaa ccaaaattat taagtgacga ttatttcttt tggcatatca aaagtgtatg
    33541 cccaacgcct aaattatcgt ggcgctctgc tacctggaat cctgctttat tcagatagtc
    33601 gtaaaatttc tctgcactat agaaacggct gttaccgttt gccatgcagg taaaataaag
    33661 tgaggacgcg ttcaggctaa acgatgctgc ttcaaaccgc tgcgcatccc agaacaactc
    33721 catgatgcaa acttttgcgc caggcttcat tacgctggca attttgctga gtatgctaat
    33781 tatttgttcg ggtgaaaaac agtctaaaaa ttggctcatc caccagatat cagcctcgcc
    33841 cggtaatggg gcatcgctta gcatatccac cgcatggaaa tctatgcgat cagaaaaacc
    33901 tgcattcgcg atattttctt tcgctagtac gatttgttgt ggcagatcta ataacgtaac
    33961 ggcgatgttt tcattgtatt tgcagcaacg caaagcccac ttacccgtat tgccgcctac
    34021 atcgtacaat gttgtggggt tgttggcgaa tacataaggc agtgcggcgt taaatgcgcc
    34081 atctgaatag taatgatcaa acgcaaacca gctttcacga gcggcgtctg gtaattgcga
    34141 aagtgccgga tatatcgtcg accagtcgcc aaatactttt aacccggaag gttttccttc
    34201 attgagtgaa tcggcgagga agaagagccc ctggtaacag acatcctggg tgaaatccat
    34261 attcacgcga gtcatagtgt catgcaggag gtaatgaccg attttcgcca ggtaataaat
    34321 tccctcttta caggtaataa ttcgcccgct taagcccata tctaacagaa cgctggtagc
    34381 atattcgttg atatggctat gttcggtgat atcagccagc gttgcgccat gtttgccttg
    34441 ccgatccaga tatgaaagta cacccgcatt acgtaggcag agtgctgttt gaaaaagcat
    34501 aggggcaaag gcaatgcgtt gagcttcggt aatcgcatcc agtgcgctaa gagaatcctg
    34561 ttcgtacatg ttgggcacaa cctgttaaga gggaattaaa ttattatcgg accgcaacgg
    34621 cccgacttta ggaagaatta taaatctgcg cccgctgaca aattcagttg aatatctttg
    34681 tcgagattgc ggacccagcg attgtagttt ttatgaattt gtccgcctga cgcttgtagg
    34741 ttctggctat tttcataatt gatatcgtag ctggatgccg tgtagttaat gcgtatttcc
    34801 gctgaatgat cgcgtgactg ttgacgtgct ttaattatgc cttgtccggc ttcggacata
    34861 atccatttgc gttcaacgcc agctttcaga atcgcttttt tcacttgttc ctgggtatga
    34921 ccagcgctga cggttgagtg aacctgatca attggcgcag tacgcgcgca acctgctaaa
    34981 gcacccacaa cagcaatagc cgcaaaccat ttcatgtaat gtctcattag aaccccatta
    35041 aagtattgtt tttacgagta aagcaagttt taactgtaat tgctggcatc actattcttt
    35101 atttagagaa aaaattactt gtgagataag tcagtaagtg cacatcagag taaaaatctc
    35161 tgaaatgact aaattactgc cagcagggac caactataag tgagtgagaa aaatgaggca
    35221 ataaaaataa cacaatgtta tcagtgcatt ctttgagaag gatttaatcg ctattcggcg
    35281 tttatatata tttgacttaa ggaatggttt ttttaaatta attttctgaa taatgtttca
    35341 tcatttcctc aaatcaaatg gccactgtta gataaacggt ggccattgcc tgctcaggag
    35401 gtgctattaa cccggtaacc gacttttcgg tctgcccggc cccagtaaaa tcgccagttt
    35461 gctgccgcct ttcgtcgttt ccatccagat tttacacacg ctggttaatg gcaccgaaag
    35521 tagcatccct accgggccga gcagccatcc ccaaattaac aatgaaagaa ataccaccat
    35581 ggtggacatc cccaggcgat ggcccatcat ccgtggttct aaaatattgc cgatgaccat
    35641 atggaccact aaaaacaatg cgccgaccag aatacattcg taaacaccat taaacagcag
    35701 cacctgaatc attggcggta cggcggaaat taccgcgccg atattgggca cgtagttaag
    35761 caaaaacgcc agtactgccc acatcagcgc aaactgtacc cccatcagct ccagccccag
    35821 ccagacgatg acacccgtcc ataaactgag tagcgtcttt aacgccagat aatgcgaaac
    35881 gcctttaagt gcgcggtgta atcccgcgat gtgaatctgt gggttattca gcgcaaaacg
    35941 cattttgtaa gggacgtggc gcacttcaaa cagcataaaa actacggtca tcaccagcaa
    36001 aagcacgctc gccattgccc cggaaagccc ggtcattagc gctgtggtga aggtaaccac
    36061 tttttccgag tccatccgct gcagcattcg ctccggcgac atatgcaaat taagaaaagg
    36121 caacatctcc tgcaatttaa aaagtttgcg cgtcagctcc ttattaaact tcggcagcat
    36181 agagataaat tcgttaaacg atgccgccag tacgccgacc agcgcggtta gtgcgatcag
    36241 catcaccacc actacaatcg taatggcaac ggggcgttgt actccccgac gaataaacca
    36301 ggtgacgagc gggttgagga cgatggcaaa aaacagcgcc agtaacagct gcacaatgat
    36361 atctgccgct gcgtgaatgc ccgcgaggat cactaccagc gaggccagct tgagcagaat
    36421 gtgcatgccc gttttatcgg gttgaggggt ttccattggg gcttccttgt gactttttgt
    36481 attaagtgta gcgggagtca cgacggcaat attctgattc ttactgctga ttttcacgtc
    36541 agcacgtggt aaaaatgaaa cactgttgta aaaatgtggt gatcctcatg cccgaacccg
    36601 tagccgaacc cgcgctaaac ggattgcgcc tgaatttgcg cattctctcc attgtcatgt
    36661 ttaacttcgc cagctacctc accatcgggt tgccgctcgc tgtattaccg ggctatgtcc
    36721 atgatgtgat gggctttagc gctttctggg caggattggt tatcagcctg caatatttcg
    36781 ccaccttgct gagccgtcct catgccggac gttacgccga tttgctgggc cccaaaaaga
    36841 ttgtcgtctt cggtttatgc ggctgctttt tgagcggtct gggatatctg acggcaggat
    36901 taaccgccag tctgcccgtc atcagcctgt tattactttg cctgggacgc gtcatccttg
    36961 ggattgggca aagttttgcc ggaacgggat cgaccctgtg gggtgttggc gtggttggct
    37021 cgctgcatat cgggcgggtg atttcgtgga acggcattgt cacttacggg gcgatggcga
    37081 tgggtgcgcc gttaggcgtc gtgttttatc actggggcgg cttgcaggcg ttagcgttaa
    37141 ttattatggg cgtggcgctg gtggccattt tgttggcgat cccgcgtccg acggtaaaag
    37201 ccagtaaagg caaaccgctg ccgtttcgcg cggtgcttgg gcgcgtctgg ctgtacggta
    37261 tggcgctggc actggcttcc gccggatttg gcgtcatcgc cacctttatc acgctgtttt
    37321 atgacgttaa aggttgggac ggtgcggctt tcgcgctgac gctgtttagc tgtgcgtttg
    37381 tcggtacgcg tttgttattc cctaacggca ttaaccgtat cggcggctta aacgtagcga
    37441 tgatttgctt tagcgttgag ataatcggcc tgctactggt tggcgtggcg actatgccgt
    37501 ggatggcgaa aatcggcgtc ttactggcgg gggccgggtt ttcgctggtg ttcccggcat
    37561 tgggtgtagt ggcggtaaaa gcggttccgc agcaaaatca gggggcggcg ctggcaacct
    37621 acaccgtatt tatggattta tcgcttggcg tgaccggacc actggctggg ctggtgatga
    37681 gttgggcggg cgtaccggtg atttatctgg cggcggcggg actggtcgca atcgcgttat
    37741 tactgacgtg gcgattaaaa aaacggcctc cggaacacgt ccctgaggcc gcctcatcat
    37801 cttaaaactt actgaataac cagcgtatta atgatgtttt ctgcggtggt ctgcgctttt
    37861 tgctgatcgt cagctggcag agtaatttgc atggtcagca gttgattacc cacgttaccc
    37921 agaataacgg aagagtacgc cgtctggcct ttcgcggaga taatactgtc taactgctgc
    37981 attttgtgac ctttcagctc aatggcttta ttggtgacca cttgcagctg tggatcgcgg
    38041 ctacgttgct gatcttccag acgcttcgcc agcaccgcca gatcttcttt cggatcatcg
    38101 cccatgatga cgatgactgc tttctgcccg gtggcgtcgg accagacatg catgttattg
    38161 gcctgcgttc ccagcttacc gctctggtcg gtcatatccg ctggcagcga gaaacttaac
    38221 ttgccatcaa gcaggttgac gggattcccg gtagcgttac tttccgcgac cgaaccctgc
    38281 gccgtagcgt tagtgtcttt atcatcacag gccgcaagcc ccataaccag caggccaatt
    38341 ccgacatatt taaccagatt gcgcattgac ttcttccttt cgataaacgg ccataacggc
    38401 tcattcatcc atcttatcac aactctgata acgaaccttt aactcgcctg caaagcgttg
    38461 atttcggatt tatctgccag tcttttcaac agcatattga gtaatacgcc atacattggc
    38521 aggaagaaaa cgatactgat taacactttg aaacagtaat cgaccagcgc gatttccatc
    38581 cagtgttcag ccataaaggc atccgggcta cgccagaagg caatgaagaa aaaggccagc
    38641 gtgtcgctga cgttaccgaa cagtgtggac gctgtcggtg ccagccacca gcggcgactc
    38701 tgacgcaggc ggttaaaaac gtgcacgtcg aggatctgtc ccagcgcgta ggccatgaaa
    38761 ctggcggtgg cgatacgggc gacaaacagg ttgaagtggg cgagtgcgcc gaatccctgc
    38821 catgaaccca tatagaatag cgacgagatg acgtaggaga tcagcaatgc cgggatcatc
    38881 accgcaaaga tgatacgacg ggccagcggt gcgccaaaaa tacgtacggt caggtcggta
    38941 gcgagaaaga taaacggaaa gctaaacgcg ccccaggtgg tatggaaacc aaaaatggag
    39001 acgggaagct gaaccagata gttactggag gtgatcacca gcagatgaaa taacgataac
    39061 cagaacaacg ccttatagcg ttgagtttgc gagaaaacgt tcatattgta cctttttgat
    39121 taaccattgg ggtgagggaa cccaatacgt acgacacgtc tgttacctta acataataac
    39181 gtgcttaccc ttttttcgag ccgccgcatg atactgcttt acgttgacaa tgcaatggtt
    39241 aattttcacg caatcgttaa cctggtttgc ttacggactc acagggcgta aactagcgcc
    39301 gtttttttat gtgatgagaa gaaaatgacc gatctctttt ccagccctga ccacacactc
    39361 gacgcacttg gcctgcgctg cccggaacca gtcatgatgg tgcgcaaaac agtgcgcaat
    39421 atgcagccag gtgaaacatt gctgattatt gctgacgatc cggcgacgac ccgcgatatc
    39481 cctgggtttt gtacctttat ggaacacgaa ctggttgcca aagagacgga tggactgcct
    39541 tatcgttatt tgattcgtaa aggcggttga cgggggcaga ttggcttcga tgccgccttt
    39601 tcccctcacc ctaaccctct ccccagagga gcgaggggac cgatcgcgct caatgttgcg
    39661 atcggtttgc cttatctcct gcgcaacaat cttaacgcat tcgctgtcac cagcaccgtc
    39721 gcacccgtat ctgccagcac tgccagccat agtccggtca tccctaacag cgtggtgacg
    39781 aggaagatcc ctttcagccc cagcgcaata gtgatgttct ggcggatatt ggcgtgagtg
    39841 gcgcgtgcca gttcaatcat ttgcaccaga ccgcgcaggt ggttatgggt taatgctgcg
    39901 tcggcggttt ccagcgccac gtctgtgccg ctgcccattg caatcccgat ggcggcagct
    39961 ttcatcgctg gcgcgtcgtt aataccgtca ccgaccatcg ccagcggcgc atgttgattc
    40021 agctcggtca ccgctttgac tttatcttcc ggcaacaggc ccgctttaaa ctccagcccc
    40081 agctccccgg caattgccgc cgctgcgcgt ggattatcgc cggtgaggat cacccctttg
    40141 acgcccagcg cgttcagttc actgatggca gttgcagcat cggcgcgcag ggtatcctgc
    40201 aatgcaatga tacccagcac gtcatcatta cgtactacca gcacaaccgt ttgcccggcg
    40261 ctttccagtt cattaatcaa accagcaaat gcatcagcgg gatgtttccc ggcagcgcat
    40321 atcaacacgc gctcaccgtt aacctgcgct tcaatgccag acccgaccag cgcccgctgt
    40381 gattcggcgg cgggaatggc gagtgcagca acctgtgctt cgcgcacgat ggcctgcgcc
    40441 agtggatgcg tcgcgccttg ctcgaccgcc gccgccagtg tcagcagttc agattcacta
    40501 atacccgttg ccggatgaat cgcggtaacg cgcggtttac cgacggtcag cgtaccggtt
    40561 ttatcaaacg ccacctgggt aacacgaccc agctgttcca gcgccgctcc gcctttaatc
    40621 aacgccccac gacgcgctgc cgccgccagc ccggaggtaa tcgccgcagg cgttgagata
    40681 actaacgcac acgggcagcc aatcagcagc agcgtcagcc ctttataaat ccactcctgc
    40741 cagctggtgg caaacagcag cggtggcacc agcgtcacca gcagagcgac ggccataatc
    40801 gcgggcgtgt aaatacggct gaaacggtcg ataaaccgct caatgggtgc gcgacgctct
    40861 tcggcttctt cgatcagttt cagaatccgg tcaatggcgc tggcacccgg ttctgacagc
    40921 acttccagcg ttaccagacg gtctacgctg gtggctcctg caggaacttt atcgcccgtt
    40981 gcgcgctcca ccggaataga ttcgccggtc agggcgcttt catcaaaact ggcaaacggt
    41041 gagagcagtt taccgtcggc aggcaaacgt ccacctgcgg cgacttcaat cacatcgcca
    41101 gggcgcaggc tgttaatcgc cacctcttcc cgctcaccgt tacgcaggcg cgtggcggtt
    41161 tctggtttca gcgccattaa cgcgctgacc ccttgacgcg cgcggctggc ggcccagcct
    41221 tccagtcgtt caccaatcaa aaacagcagc aacaccatcg cagcttcagc cgttgcgcca
    41281 ataaacagtg caccaatagc ggctacgctc attaaggttt caatggcgaa gtagctgccg
    41341 gatttgatca gccgtaatgc ctgacgagca atcgggtaca gcccaaccag cgtggtcgcg
    41401 ataaacgcca gttgcccgaa cggatgatta aattgctcca aaccccagct gattgccatc
    41461 atcacgataa gcgtaatcag cggcagattc tctttcaggc gcgatgcttg tggctcgtcg
    41521 gcggcctgct catcgcgcag ggaatagccc gctttttgca ccgcagattc aacttgtgca
    41581 cggatgtcat tgtcggcatc aaccaccagt ttttcggtgg cgaacaacac ctgtacctga
    41641 ttcacgcctg caagctggcg cacggcattt tctaccttgc gcgcacaggc ggcgcagtcc
    41701 atgccgctga ctttccagct atagcgggtg ccggagacgt tttcagagag agttggcgtg
    41761 ctggaacatg cgccgtcgca gcaacagtcg ttggcgttct gtaccgtggt tagcggtttg
    41821 aacgcagcaa attgaggggc tttcttgccg tgattgtcag gagtcgacat ggcatcctcc
    41881 ggttaagttt tttctcatta accgaaggat acactctgga gtcgactcca gagtcaagtt
    41941 ttatcagaga tacagcgagc ggacgatgag gaagtgaccg gcaaagtagc aggccgcagc
    42001 aatcgcgttg tccgcacgga agcgtcggcg atagtgacta cccagccaga caaagttact
    42061 gataaacagc aacgaagcgc cgacaaacgc agagagtgcc ggagcggtcg gacggaagaa
    42121 ccacagttca cctgccagcc acaccatcac cagcgtcatg ccgataaacg tgcagatagg
    42181 ccaacgatac tcttccagcc gcgtccagat aatcgccagt aacagcgcac ccagaaccag
    42241 caacaccagt ggcagaggcc agaagaaaga gagcgtcatc tgactggcga aatagatggt
    42301 gtacagcagg tgcgagagga aaaacgcgcc gatggcgtac atcagacgtt gacgtggcaa
    42361 cagggttagc gcatcgccca gcagtgaggc gcacagccct gccagcacca gatagctaat
    42421 ggcgtcgaac atcggcgctt gccaggccag taacagcagg agaagaaggg ttaacggttt
    42481 aaacacccag cgttgccagg ttggcccacg atacgatgca tccacagata gccatgcgga
    42541 aagacagaca gcgataaacg accaaagcat cttagttcct tgaattcgtt attttttcgt
    42601 aagcggaatg cctacggtct tgcttccagt ttagtggcga gggtggacgg atgacaacac
    42661 cataactggc agggtatgct tatttccgca ttttccgatg agggataaag atgagcaaac
    42721 cacctctttt ctttattgtt atcattggct taatcatcgt cgccgcatcg tttcgtttta
    42781 tgcaacagcg acgggaaaaa gctgataatg atatggcacc actccagcaa aagctggtgt
    42841 tggtgagcaa caagcgggaa aaaccgatta acgatcgccg ttcgcgccag caggaagtga
    42901 ctccggcagg caccagtatg cgctatgagg caagcttcaa accgcaaagc ggaggaatgg
    42961 agcagacgtt tcgcctcgac gcccagcagt accacgccct gacagtgggc gataaaggta
    43021 cgctgagcta taaaggaacg cgctttgtta gctttgttgg cgaacaataa ggttattttt
    43081 taactttaaa tttcttctgc cagaccagca gttcaaacac gccgaaaagg aaaatccgca
    43141 ccttttcggc ggtggtcatc tgcgggccat ctttcggtaa agtggatttc aatagcgcca
    43201 gttgcatacc atgcatcagc acggtaaaaa tcagcgccac gttaacgaaa atattcagcg
    43261 ggcgcgggaa gggatgcacc aggttgagga ttaaaaatcc ccaaacacag agcattaaca
    43321 aacgaccaat attaatcagc atcactttct ccttgtgctt cgcgttgata cagccgataa
    43381 gccacctgac ccgccacttt ttcccgatgt aacgaccagt ttgccggaac ggtgggcaga
    43441 ccgttttcga cttcgctttc gacataaatc agggtttcgt cagccagcca gccgttatct
    43501 tccagtaaat ttatcgtctc ttctaacaaa ccacggcgga acggtggatc gacaaacacg
    43561 atattatgcg gtgtgccttt ttgcgccagg aatgacattg cgttgctgtt caccacgcgt
    43621 gcattgcctg cttttagtgt cgccagattc ttaattaact gctgagaaac tgcacgatcc
    43681 atctcaatta acgtggcccc cgcagcgtag cgcgataacg cttccagccc cagcgcgccg
    43741 ctcccggcga agcaatccag acattgggcg tcaacaatga ccggagccag ccagttaaac
    43801 aacgtttcgc gtacgcggtc ggtggtgggg cgcagacctg ggctatcagg aaccgggagt
    43861 ttacggcctc gccactgccc gccaataatg cggatttggc cgctgccgga atgattcggt
    43921 tttttcatga taaattgctc aatccgccag ataacacata cttgcaggcg gtgatgtgaa
    43981 ttaagttaag tgatagacta tttcatcatt tttttagctg ctatgtacat agcgttaacg
    44041 ctgtgccatg aagcaacagc gaggagtgta gtcgcaaatg gcgaaagaaa aaaaacgtgg
    44101 ctttttttcc tggctgggct ttggtcaaaa agagcagacc ccggaaaaag agacagaagt
    44161 tcagaatgaa caaacggttg tagaagaaat cgttcaggcg caagagcctg tgaaggcttc
    44221 tgaacaagcc gtcgaagagc agccgcaggc gcatactgag gccgaggcgg aaacttttgc
    44281 tgccgacgtt gtggaagtca ctgaacaggt tgctgaaaat gaaaaagcgc agcctgaagc
    44341 ggaagtcgtt gcacaaccgg aaccggtcgt agaagaaacg ccggagccag tggctatcga
    44401 acgtgaagag ctgccgttgc cggaagacgt caacgccgaa gaggtttcgc cagaagagtg
    44461 gcaggctgaa gcggaaaccg tagagattgt cgaagcggcg gaagaagaag cggctaaaga
    44521 agaaatcacc gacgaagagc tggaagcaca ggcgctggct gccgaagcag cagaagaggc
    44581 ggtgatggtg gttcctccgg cagaagaaga gcagccggtg gaagaaatcg ctcaggagca
    44641 ggaaaaaccg accaaagaag gttttttcgc gcgcctgaaa cgcagcctgt taaaaaccaa
    44701 agaaaatctc ggttccggat ttatcagcct gttccgcggt aaaaaaatcg acgatgatct
    44761 gtttgaggag ctggaagagc agcttttgat cgccgatgtg ggcgtggaaa ccacacgtaa
    44821 aattatcacc aatctgacgg aaggcgcatc ccgcaagcag cttcgtgatg ccgaggcgct
    44881 ctatggcctg ctgaaagaag agatgggtga gattctggcg aaagtcgatg agccgctgaa
    44941 tgttgaaggt aaaacgccgt tcgtgatcct gatggtgggc gtcaacggtg tgggtaaaac
    45001 cacgacgatt ggtaagctgg cacgtcagtt tgagcagcag ggtaaatcgg tgatgctggc
    45061 ggcgggcgat actttccgtg cagcagcggt tgaacagctt caggtctggg ggcagcgcaa
    45121 caatattccg gtgattgccc agcataccgg ggcggattcc gcctctgtta tcttcgacgc
    45181 cattcaggcg gctaaagcgc gtaatatcga cgtcctgatt gccgataccg ccggacgcct
    45241 gcagaacaaa tcgcacctga tggaagagtt gaagaaaatc gtccgcgtga tgaagaaact
    45301 cgacgttgaa gcgccgcatg aagttatgct gactatcgat gccagcaccg ggcagaacgc
    45361 ggtaagccag gccaaactgt tccatgaagc cgttggctta accggcatca cgctaacgaa
    45421 actggacggc acggcgaaag gcggggtaat tttctcggtg gctgaccagt ttggtatccc
    45481 tatccgctac attggtgtcg gcgaacgtat tgaggatttg cgtccgttta aggcggacga
    45541 ctttatagag gcactttttg cccgagagga ttaacaatga ttcgctttga acatgtcagc
    45601 aaggcttatc tcggtgggag acaggcgctg cagggcgtta cgttccatat gcagccgggt
    45661 gagatggcgt ttctgaccgg tcattccggc gcagggaaaa gtaccctcct gaagctgatc
    45721 tgtgggattg agcggcccag cgccgggaaa atctggttta gcggccatga catcacgcgt
    45781 ctgaaaaacc gtgaagttcc gtttctgcgc cgccagattg gcatgatttt ccaggatcac
    45841 catctactga tggaccgtac tgtctacgat aacgtggcga tcccgctgat tatcgccggt
    45901 gccagcggtg acgatattcg ccgccgggtg tcggcggcac tggataaagt cgggctactg
    45961 gacaaagcga agaacttccc tattcagctt tctggcggtg aacaacagcg tgttggcatt
    46021 gcccgcgcgg tggtgaacaa gcccgcggta ctgctggcgg acgaaccgac tggtaacctg
    46081 gacgacgcgt tgtcggaagg gattctacgt ctgtttgaag agtttaaccg cgtaggggta
    46141 accgtattga tggcaacgca cgacatcaac ctgatttcac ggcgttccta tcgcatgctc
    46201 accctgagcg atggtcactt gcatggaggc gtgggccatg aataagcgcg atgcaatcaa
    46261 tcatattcgg cagtttggcg ggcgtcttga tcgcttccgt aaatcggtcg gcggctcagg
    46321 cgacggcggt cgtaacgcac cgaaacgcgc gaaatcctcg ccaaaaccgg taaatcgcaa
    46381 aaccaacgtt ttcaacgaac aggtgcgcta tgccttccac ggcgcattgc aggatctgaa
    46441 aagcaaaccg ttcgccacat ttttaacggt gatggttatc gccatctccc tgacgctgcc
    46501 cagcgtctgt tatatggtgt acaaaaacgt taaccaggcg gcgacgcagt attatccgtc
    46561 accgcaaatc actgtttatc tgcaaaaaac gctggacgat gacgccgctg cgggcgtggt
    46621 ggcacagttg caggccgagc aaggcgtaga gaaagtgaac tatctttctc gtgaagacgc
    46681 actgggcgag ttccgtaact ggtctggttt tggtggcgcg ctggatatgc tggaagaaaa
    46741 cccgcttccg gcagtggcgg tggtgatccc gaagctcgac tttcagggga cggagtctct
    46801 gaatacgctg cgtgatcgta tcacgcagat taacggcatt gacgaagtgc ggatggatga
    46861 cagctggttt gcccgtctgg cggcgttgac cgggctggtc gggcgcgttt cggcgatgat
    46921 cggcgtgttg atggtggcgg cagtgttcct cgtcatcggt aacagtgtac gcctgagtat
    46981 ctttgctcgt cgtgactcca ttaacgtgca gaaactgatt ggtgcaacgg atggtttcat
    47041 cctgcgcccg ttcctgtacg gtggcgcatt gctggggttt tctggcgcat tgttgtcatt
    47101 aattttatca gaaattctgg tactgcgatt gtcatcggcg gttgcggaag tggcacaggt
    47161 tttcggaacg aagtttgata tcaatggttt atcgttcgat gaatgcctgc tgttgctgct
    47221 ggtatgctcg atgattggct gggtggcagc gtggcttgcc acggtacaac atttacgcca
    47281 ctttacgcct gaataataaa agcgtgttat actctttccc tgcaatgggt tccgtagcag
    47341 ggaaagagac cccgttgtct cttcccggta tttcatctct atgtcacatt ttgtgcgtaa
    47401 tttattcaca agcctgcatt gaacttgtgg ataaaatcac ggtctgataa aacagtgaat
    47461 gataacctcg ttgctcttaa gctctggcac agttgttgct accactgaag cgccagaaga
    47521 tatcgattga gaggatttga atgactgaca aaatgcaaag tttagcttta gccccagttg
    47581 gcaacctgga ttcctacatc cgggcagcta acgcgtggcc gatgttgtcg gctgacgagg
    47641 agcgggcgct ggctgaaaag ctgcattacc atggcgatct ggaagcagct aaaacgctga
    47701 tcctgtctca cctgcggttt gttgttcata ttgctcgtaa ttatgcgggc tatggcctgc
    47761 cacaggcgga tttgattcag gaaggtaaca tcggcctgat gaaagcagtg cgccgtttta
    47821 acccggaagt gggtgtgcgc ctggtctcct tcgccgtcca ctggatcaaa gcagagatcc
    47881 acgaatacgt tctgcgtaac tggcgtatcg tcaaagttgc gaccaccaaa gcgcagcgca
    47941 aactgttctt caacctgcgt aaaaccaagc agcgtctggg ctggtttaac caggatgaag
    48001 tcgaaatggt ggcccgtgaa ctgggcgtaa ccagcaaaga cgtacgtgag atggaatcac
    48061 gtatggcggc acaggacatg acctttgacc tgtcttccga cgacgattcc gacagccaac
    48121 cgatggcacc ggtgctctat ctgcaggata aatcatctaa ctttgccgac ggcattgaag
    48181 atgataactg ggaagagcag gcagcaaacc gtctgaccga cgcgatgcag ggtctggacg
    48241 aacgcagcca ggacatcatc cgtgcgcgct ggctggacga agacaacaag tccacgttgc
    48301 aggaactggc tgaccgttac ggtgtttccg ctgagcgtgt gcgccagctg gaaaagaacg
    48361 cgatgaaaaa actgcgcgct gctatagaag cgtaatttcc gctattaagc agagaaccct
    48421 ggatgcgagt ccggggtttt tgttttttgg gcctctacaa taatcaattt cccctccggc
    48481 aaaacgccaa tccccacgca gattgttaat aaactgtcaa aatagctatt ccaatatcat
    48541 aaaaatcggg tatgttttag cagagtatgc tgctaaagca cgggtagtca tgcataaaac
    48601 gaaataaagt gctgaacaac aacatcacaa cacacgtaat aaccagaaga atggggattc
    48661 tcaggatgaa cataaagggt aaagcgttac tggcaggatg tatcgcgctg gcattcagca
    48721 atatggctct ggcagaagat attaaagtcg cggtcgtggg cgcaatgtcc ggtccggttg
    48781 cgcagtacgg tgaccaggag tttaccggcg cagagcaggc ggttgcggat atcaacgcta
    48841 aaggcggcat taaaggcaac aaactgcaaa tcgtaaaata tgacgatgcc tgtgacccga
    48901 aacaggcggt tgcggtggcg aacaaagtcg ttaacgacgg cattaaatac gtgattggtc
    48961 acctctgttc ttcatcaacg cagcctgcgt ctgacatcta cgaagacgaa ggcattttaa
    49021 tgatcacccc agcggcaacc gcgccggagc tgaccgcccg tggctatcag ctgatcctgc
    49081 gcaccaccgg cctggactcc gaccaggggc cgacggcggc gaaatatatt cttgagaaag
    49141 tgaaaccgca gcgtattgct atcgttcacg acaaacagca atacggcgaa ggtctggcac
    49201 gagcggtgca ggacggcctg aagaaaggca atgctaacgt ggtgttcttt gatggtatca
    49261 ccgccgggga aaaagatttc tcaacgctgg tggcgcgtct gaaaaaagag aatatcgact
    49321 tcgtttacta cggcggttat cacccggaaa tggggcaaat cctgcgtcag gcacgcgcgg
    49381 cagggttgaa aactcagttt atggggccgg aaggcgtggc taacgtctcg ctgtctaaca
    49441 ttgcgggcga atcagcggaa gggctgctgg tgaccaagcc gaagaactac gatcaggttc
    49501 cggcgaacaa acccattgtt gatgcgatca aagcgaaaaa acaggaccca agcggcgcat
    49561 tcgtttggac cacctacgcc gcgctgcaat ctttgcaggc gggcctgaat cagtctgacg
    49621 atccggctga aatcgccaaa tacctgaaag cgaactccgt ggataccgta atgggaccgc
    49681 tgacctggga tgagaaaggc gatctgaaag gctttgagtt cggtgtgttt gactggcacg
    49741 ccaacggcac ggcaaccgat gcgaagtaac agatagataa atgaaaatgc ccgatgcgct
    49801 gaactgccat cgggtttttt tgtgtttgtc gaaatggttg tagaaagatt aaacaatcca
    49861 ggatatgctt aatgtacggt gacataccgt acatattata cggagcagga gagttcacat
    49921 gtctgctgtt aaaaagcagc gtatcgatct gcgtttaact gacgatgaca aaagtatgat
    49981 tgaggaagcg gcagcgatat ctaatcagtc cgtcagccaa tttatgttga acagcgcttc
    50041 gcagcgggct gcggaagtga ttgaacagca tcggcgggtg atcctcaatg aggaatcctg
    50101 gacgcgggtg atggatgcgc tgagtaatcc accgtcacca ggtgaaaagc taaaacgtgc
    50161 ggcaaaacgt cttcagggaa tgtaataatt tatggatgat ctgacgatag agattctgac
    50221 cgatgatgca gattatgatc tacagcgatt cgactgcggc gaggaagcgt taaatctctt
    50281 tctgacgaca catctcgttc gtcaacatcg caacaaaatt ctgcgagcgt atatcctttg
    50341 tcgcaacact ccagaacgtc aggtgctggg atattacaca ttatgcggca gttgttttga
    50401 acgagccgca ttgccctcga aatcgaaaca gaaaaaaatt ccctacaaaa atattcccag
    50461 cgttactctt gggcgtctgg caattgatcg ttcattacag gggcagggat ggggagcaac
    50521 actggttgct catgccatga aagtcgtctg gtcagcctct ttagcggtag gtattcacgg
    50581 tctttttgtc gaggcgctga atgaaaaagc ccatacgttt tataaatcgc tgggctttat
    50641 ccctttagtc ggggaaaacg aaaatgcgtt atttttccca accaaatcca ttgaactgct
    50701 ttttacacag agcgattaac gcttctccca gccgccctgc tgtgccgtaa atcccagtgc
    50761 ctgcataaac gccgtcatca caccgcgatc ttccacgcct gcatccgcca tccaccagca
    50821 tgaaacgcca ggattgttac gcaaaacctc ttccagcaga tattgcccca caccgcgacg
    50881 tcgagtgact tcccgcacgc gcagggaatc cagtgccccc tcggtgccgc ttaaggttac
    50941 ccgcacggca gcaagcaggc gctcgttaaa acgcgcggcg tagatgcggt ggttatcgtc
    51001 aacctgtaaa gaggaagggg aatactccgg ccagatcttt tgcaggtcaa tccggtcctg
    51061 gtcgctaaat ttttctaatc gaatgatggt cagcttcatg ggtaacccgt gtaaatcaca
    51121 aaagtggaac cagtgtagcg aaataattta atcggaggct ttctcttttt tatttctttt
    51181 ggcaggtgat taatttctta acagcaataa ttacaaagtt aaaacattag agaatgaaaa
    51241 atacccagca taatcccctg aatggtagtg aattattccg ccctttgtgc cg
//
LOCUS       DAZKIP010000018        49996 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZKIP010000018 DAZKIP010000000
VERSION     DAZKIP010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49996
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.018"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..1778)
                     /gene="espL1"
                     /locus_tag="ACHOUK_001324"
     CDS             complement(<1..1778)
                     /gene="espL1"
                     /locus_tag="ACHOUK_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HFP9896801.1"
                     /translation="MSQNDIIIRTHYKSPHRMHIDSDIPTPSSEPINQFAPQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFTINFATSPIHAENIFITLHYKKEII
                     SLLLETTPIKANHLRSILDYIEQEQLTAENRNHCMKLSKKIHREKTIQPTVNLNSSAF
                     FSQSPSDAIFCRHLSLQYALDSLRNGKGKVNLIKHYSSVESIQQHVPLVRDAEFRALL
                     RHPPAGSRVIASKDFGFALDIFFCRMMANNVSHMSAILYIDNHTLSVRLRIKQSAYGQ
                     LNYVVSVYDPNDTNVAVRGTHRTARGFLSLDKFISSGPDAQTWADRYVRNCAIAILPL
                     LPEGVPGAIFTGIATRMPFAPIHPSAMLLIMATGQTQQLITLFRQLPILPEKEIIEII
                     TAQNSIGTPALFLAMMNGHTDNVKIFMQEIQSLVDNHIIHEDNLVKLLQTKSANETPG
                     LYISMLYGFDEIIDIFLNALTTPIAQELLNKKLVMSILAMKIHDGEPGLYAAMENNHP
                     LCVTRFLSKINGIAFKYKLSKANIMDLLKGATAQGTPALYIAMSKGNEDVVLSYISTL
                     GAFAKKHSFSQHQLFTLLAAKNHDNMS"
     gene            2301..4229
                     /gene="thrS"
                     /locus_tag="ACHOUK_001325"
     CDS             2301..4229
                     /gene="thrS"
                     /locus_tag="ACHOUK_001325"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFP9896802.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKIAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            4233..4775
                     /gene="infC"
                     /locus_tag="ACHOUK_001326"
     CDS             4233..4775
                     /gene="infC"
                     /locus_tag="ACHOUK_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFP9896803.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            4872..5069
                     /gene="rpmI"
                     /locus_tag="ACHOUK_001327"
     CDS             4872..5069
                     /gene="rpmI"
                     /locus_tag="ACHOUK_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFP9896804.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            5122..5478
                     /gene="rplT"
                     /locus_tag="ACHOUK_001328"
     CDS             5122..5478
                     /gene="rplT"
                     /locus_tag="ACHOUK_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFP9896805.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    5595..5719
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            5601..5645
                     /gene="pheM"
                     /locus_tag="ACHOUK_001329"
     CDS             5601..5645
                     /gene="pheM"
                     /locus_tag="ACHOUK_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFP9896806.1"
                     /translation="MNAAIFRFFFYFST"
     gene            5928..6911
                     /gene="pheS"
                     /locus_tag="ACHOUK_001330"
     CDS             5928..6911
                     /gene="pheS"
                     /locus_tag="ACHOUK_001330"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFP9896807.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            6926..9313
                     /gene="pheT"
                     /locus_tag="ACHOUK_001331"
     CDS             6926..9313
                     /gene="pheT"
                     /locus_tag="ACHOUK_001331"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFP9896808.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHNGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQYKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            9318..9617
                     /gene="ihfA"
                     /locus_tag="ACHOUK_001332"
     CDS             9318..9617
                     /gene="ihfA"
                     /locus_tag="ACHOUK_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFP9896809.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            9718..10698
                     /gene="btuC"
                     /locus_tag="ACHOUK_001333"
     CDS             9718..10698
                     /gene="btuC"
                     /locus_tag="ACHOUK_001333"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFP9896810.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     SPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPMLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRALL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            10761..11312
                     /gene="btuE"
                     /locus_tag="ACHOUK_001334"
     CDS             10761..11312
                     /gene="btuE"
                     /locus_tag="ACHOUK_001334"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFP9896811.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLISAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            11312..12061
                     /gene="btuD"
                     /locus_tag="ACHOUK_001335"
     CDS             11312..12061
                     /gene="btuD"
                     /locus_tag="ACHOUK_001335"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFP9896812.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     DVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            12139..12603
                     /gene="nlpC"
                     /locus_tag="ACHOUK_001336"
     CDS             12139..12603
                     /gene="nlpC"
                     /locus_tag="ACHOUK_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFP9896813.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            12850..13563
                     /gene="ydiV"
                     /locus_tag="ACHOUK_001337"
     CDS             12850..13563
                     /gene="ydiV"
                     /locus_tag="ACHOUK_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFP9896814.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            13626..15062
                     /gene="selO"
                     /locus_tag="ACHOUK_001338"
     CDS             13626..15062
                     /gene="selO"
                     /locus_tag="ACHOUK_001338"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFP9896815.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPDKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLNDYEPGFICNHSD
                     HQGRYSFDNQPAVALWILQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(15066..15257)
                     /gene="hemP"
                     /locus_tag="ACHOUK_001339"
     CDS             complement(15066..15257)
                     /gene="hemP"
                     /locus_tag="ACHOUK_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFP9896816.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(15389..16435)
                     /gene="aroH"
                     /locus_tag="ACHOUK_001340"
     CDS             complement(15389..16435)
                     /gene="aroH"
                     /locus_tag="ACHOUK_001340"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFP9896817.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRINHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(16592..17425)
                     /gene="ppsR"
                     /locus_tag="ACHOUK_001341"
     CDS             complement(16592..17425)
                     /gene="ppsR"
                     /locus_tag="ACHOUK_001341"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFP9896818.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            17758..20136
                     /gene="ppsA"
                     /locus_tag="ACHOUK_001342"
     CDS             17758..20136
                     /gene="ppsA"
                     /locus_tag="ACHOUK_001342"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFP9896819.1"
                     /translation="MSNNGSSPLVLWYNQLGMKDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(20193..21839)
                     /gene="fadK"
                     /locus_tag="ACHOUK_001343"
     CDS             complement(20193..21839)
                     /gene="fadK"
                     /locus_tag="ACHOUK_001343"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFP9896820.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFTPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCSCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(21896..22189)
                     /gene="ydiT"
                     /locus_tag="ACHOUK_001344"
     CDS             complement(21896..22189)
                     /gene="ydiT"
                     /locus_tag="ACHOUK_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFP9896821.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(22186..23475)
                     /gene="ydiS"
                     /locus_tag="ACHOUK_001345"
     CDS             complement(22186..23475)
                     /gene="ydiS"
                     /locus_tag="ACHOUK_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9896822.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDILVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLADIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(23531..24469)
                     /gene="ydiR"
                     /locus_tag="ACHOUK_001346"
     CDS             complement(23531..24469)
                     /gene="ydiR"
                     /locus_tag="ACHOUK_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFP9896823.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSSLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(24489..25253)
                     /gene="ydiQ"
                     /locus_tag="ACHOUK_001347"
     CDS             complement(24489..25253)
                     /gene="ydiQ"
                     /locus_tag="ACHOUK_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFP9896824.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYMVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFGKGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            25569..26480
                     /gene="ydiP"
                     /locus_tag="ACHOUK_001348"
     CDS             25569..26480
                     /gene="ydiP"
                     /locus_tag="ACHOUK_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9896825.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENHLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLAG"
     gene            complement(26523..27674)
                     /gene="ydiO"
                     /locus_tag="ACHOUK_001349"
     CDS             complement(26523..27674)
                     /gene="ydiO"
                     /locus_tag="ACHOUK_001349"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFP9896826.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(27688..29283)
                     /gene="ydiF"
                     /locus_tag="ACHOUK_001350"
     CDS             complement(27688..29283)
                     /gene="ydiF"
                     /locus_tag="ACHOUK_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFP9896827.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SDLAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGSNVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLNIVQEGRVKKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(29428..30186)
                     /gene="aroD"
                     /locus_tag="ACHOUK_001351"
     CDS             complement(29428..30186)
                     /gene="aroD"
                     /locus_tag="ACHOUK_001351"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFP9896828.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLALLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(30217..31083)
                     /gene="ydiB"
                     /locus_tag="ACHOUK_001352"
     CDS             complement(30217..31083)
                     /gene="ydiB"
                     /locus_tag="ACHOUK_001352"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFP9896829.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDKF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENKSLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(31095..32360)
                     /gene="ydiN"
                     /locus_tag="ACHOUK_001353"
     CDS             complement(31095..32360)
                     /gene="ydiN"
                     /locus_tag="ACHOUK_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9896830.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(32587..33801)
                     /gene="ydiM"
                     /locus_tag="ACHOUK_001354"
     CDS             complement(32587..33801)
                     /gene="ydiM"
                     /locus_tag="ACHOUK_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9896831.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQSNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFSFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(33901..34257)
                     /gene="ydiL"
                     /locus_tag="ACHOUK_001355"
     CDS             complement(33901..34257)
                     /gene="ydiL"
                     /locus_tag="ACHOUK_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFP9896832.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGRCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(34393..34500)
                     /gene="rprA"
                     /locus_tag="ACHOUK_001356"
     ncRNA           complement(34393..34500)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHOUK_001356"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(34686..35798)
                     /gene="ydiK"
                     /locus_tag="ACHOUK_001357"
     CDS             complement(34686..35798)
                     /gene="ydiK"
                     /locus_tag="ACHOUK_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFP9896833.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIQKPNK"
     gene            36187..39243
                     /gene="ydiJ"
                     /locus_tag="ACHOUK_001358"
     CDS             36187..39243
                     /gene="ydiJ"
                     /locus_tag="ACHOUK_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFP9896834.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGLRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCARLDELIASHQAGVIGWQMCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            39240..39650
                     /gene="menI"
                     /locus_tag="ACHOUK_001359"
     CDS             39240..39650
                     /gene="menI"
                     /locus_tag="ACHOUK_001359"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFP9896835.1"
                     /translation="MIWKRKIPLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEISDEKGRLCCSSRLTTAIL"
     gene            39750..39938
                     /gene="ydiH"
                     /locus_tag="ACHOUK_001360"
     CDS             39750..39938
                     /gene="ydiH"
                     /locus_tag="ACHOUK_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFP9896836.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            40487..40855
                     /gene="sufA"
                     /locus_tag="ACHOUK_001361"
     CDS             40487..40855
                     /gene="sufA"
                     /locus_tag="ACHOUK_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFP9896837.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAVHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            40864..42351
                     /gene="sufB"
                     /locus_tag="ACHOUK_001362"
     CDS             40864..42351
                     /gene="sufB"
                     /locus_tag="ACHOUK_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFP9896838.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            42361..43107
                     /gene="sufC"
                     /locus_tag="ACHOUK_001363"
     CDS             42361..43107
                     /gene="sufC"
                     /locus_tag="ACHOUK_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFP9896839.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            43082..44353
                     /gene="sufD"
                     /locus_tag="ACHOUK_001364"
     CDS             43082..44353
                     /gene="sufD"
                     /locus_tag="ACHOUK_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFP9896840.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLELAEGAEATVIEHFVSLNDARHFTGARFSINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENCTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLTRIGQRLPGGAR"
     gene            44350..45570
                     /gene="sufS"
                     /locus_tag="ACHOUK_001365"
     CDS             44350..45570
                     /gene="sufS"
                     /locus_tag="ACHOUK_001365"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFP9896841.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSAQGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            45583..45999
                     /gene="sufE"
                     /locus_tag="ACHOUK_001366"
     CDS             45583..45999
                     /gene="sufE"
                     /locus_tag="ACHOUK_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFP9896842.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDVVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            46148..47152
                     /gene="ldtE"
                     /locus_tag="ACHOUK_001367"
     CDS             46148..47152
                     /gene="ldtE"
                     /locus_tag="ACHOUK_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFP9896843.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVAVSSGATPTASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(47215..47451)
                     /gene="lpp"
                     /locus_tag="ACHOUK_001368"
     CDS             complement(47215..47451)
                     /gene="lpp"
                     /locus_tag="ACHOUK_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFP9896844.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(47762..49174)
                     /gene="pykF"
                     /locus_tag="ACHOUK_001369"
     CDS             complement(47762..49174)
                     /gene="pykF"
                     /locus_tag="ACHOUK_001369"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFP9896845.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(49182..49376)
                     /locus_tag="ACHOUK_001370"
     CDS             complement(49182..49376)
                     /locus_tag="ACHOUK_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9896846.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            49731..49940
                     /gene="fumD"
                     /locus_tag="ACHOUK_001371"
     CDS             49731..49940
                     /gene="fumD"
                     /locus_tag="ACHOUK_001371"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFP9896847.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT        12738 a        11966 c        12801 g        12491 t
ORIGIN      
        1 gacatgttgt catgattttt agcagccaac agtgtaaata actgatgttg actaaaagaa
       61 tgtttttttg caaaagcacc cagcgtcgat atataagata acacgacgtc ttcattaccc
      121 ttgctcatag cgatgtataa tgcaggggtt ccctgtgctg tagcgccttt taataaatcc
      181 atgatgttag ctttgctcaa cttgtattta aaggcgatgc cattaatttt agagaggaac
      241 cgtgtgacac acaaagggtg attattttcc attgcggcgt ataatcctgg ctcaccatca
      301 tgtattttca tggctaaaat actcatcacc agttttttgt ttaaaagctc ttgtgctata
      361 ggagtggtta atgcattcag aaagatatcg attatctcat cgaatccata caacatggag
      421 atataaagtc caggtgtttc gttagcactt ttagtttgca acaatttaac cagattatct
      481 tcatgaatga tgtgattatc taccagtgac tgaatttctt gcataaatat tttcacgttg
      541 tcagtatgtc cgttcatcat agccaaaaat aaagcaggtg taccaatgct attctgcgca
      601 gttattattt caatgatttc tttttcaggg agtatgggca actgtctgaa taatgtaata
      661 agctgttgag tctggcctgt ggccattatt aacaacattg ccgatggatg tataggggca
      721 aatggcattc gtgtcgcgat acccgtgaaa atagcccctg gaactccctc aggtaatagg
      781 ggcagaatag caattgcaca gttgcgaaca tacctatcag cccaggtctg agcatcggga
      841 cctgaactga tgaacttatc aagcgagaga aagccccgtg ctgtcctgtg ggtgcctctg
      901 acggcaacgt tggtatcgtt cgggtcgtaa acggacacaa cataatttaa ttgcccatac
      961 gccgactgct ttattcgtag ccttactgac aaagtatgat tgtctatata taaaatcgca
     1021 gacatatgac tgacattgtt tgccatcatt cgacagaaga aaatatctaa agcgaagcca
     1081 aaatccttac tcgcgataac gcgactccct gcaggaggat ggcgaagtaa tgctctgaac
     1141 tccgcgtccc ggactaaggg gacatgctgc tgtatggatt caacggagga gtaatgttta
     1201 atcaggttga ccttgccttt tccatttctc aatgaatcaa gtgcgtattg caatgacaga
     1261 tggcgacaaa aaatagcgtc agaaggagat tgcgaaaaaa atgcactact attgagattt
     1321 actgttggtt gtatagtttt ttctctatgg atttttttag acagtttcat acaatggtta
     1381 cgattttcgg cagttaactg ttcctgttca atataatcca gtatgcttcg caaatggtta
     1441 gctttaatag gcgtggtttc cagtaataag gaaatgattt cttttttata gtgtaatgta
     1501 atgaatatat tttctgcatg gatgggtgaa gtggcaaagt taatggtaaa aagggcagtg
     1561 gaataacaat tttgtgatat ttttcgacag tttgcggtaa attcctgagt aacacagtat
     1621 gacagcatcg aacttaagtc agaggtatca agtagggtga tgagttgggg cgcaaattga
     1681 ttaataggct ctgatgaagg cgttggtatg tcgctatcga tgtgcattct atgaggagac
     1741 ttataatgag ttctgataat aatgtcgttt tgactcataa tctattcgcc ttatatatgt
     1801 ctggaggttt tgagtatgat gattaattat tatcgccaga acgaaatgtg cacgaaaaat
     1861 tgctataaga attgtgtcac ttttatcatt gtgatatgac cacttcgtca ttaatcttat
     1921 gatcgtttaa atagtaccgc caaatcacgc aacgaacttt gctggattca gtttatgctg
     1981 ctgtaaattc gctcgcagta aacctttcag acgctccgtg atgttatcag ttgttcttta
     2041 agcgttttgc tggtgtactc actacaaacg aattgcgaat cgaatcaatg tgaaacggaa
     2101 aggtacaatc tccctttttt tagtgttaac atcgctcaac cgggtggaag gtgagaggtt
     2161 aatgtctttc atttgtgtac ctgattgcga accaatttag catttgttgg ctagatggtt
     2221 tcgcaatgaa ctgttaataa acaaattttt ctttgtatgt gatctttcgt gtgggtcacc
     2281 actgcaaata aggatataaa atgcctgtta taactcttcc tgatggcagt caacgccatt
     2341 acgaccacgc tgtaagcccc atggatgttg cgctggacat tggtccaggt ctggcgaaag
     2401 cctgtatcgc agggcgcgtt aatggcgaac tggttgatgc ttgcgatctg attgaaaacg
     2461 acgcacaact gtcgatcatt accgccaaag acgaagaagg tctggagatc attcgtcact
     2521 cctgtgcgca cctgttaggg cacgcgatta aacaactttg gccgcatacc aaaatggcaa
     2581 tcggcccggt tattgacaac ggtttttatt acgacgttga tcttgaccgc acgttaaccc
     2641 aggaagatgt cgaagcactc gagaagcgga tgcatgagct tgctgagaaa aactacgacg
     2701 tcattaagaa gaaagtcagc tggcacgaag cgcgtgaaac tttcgccaac cgtggggaga
     2761 gctacaaagt ctccattctt gacgaaaaca tcgcccatga tgacaagcca ggtctgtact
     2821 tccatgaaga atatgtcgat atgtgccgcg gtccgcacgt accgaacatg cgtttctgcc
     2881 atcatttcaa actaatgaaa acggcagggg cttactggcg tggcgacagc aacaacaaaa
     2941 tgttgcaacg tatttacggt acggcgtggg cagacaaaaa agcgcttaac gcttacctgc
     3001 agcgcctgga agaagccgcg aaacgcgacc accgtaaaat cggtaaacag ctcgacctgt
     3061 accatatgca ggaagaagcg ccgggtatgg tattctggca caacgacggc tggaccatct
     3121 tccgtgaact ggaagtgttt gttcgttcta aactgaaaga gtaccagtat caggaagtta
     3181 aaggtccgtt catgatggac cgtgtcctgt gggaaaaaac cggtcactgg gacaactaca
     3241 aagatgcaat gttcaccacg tcttctgaga accgtgaata ctgcattaag ccgatgaact
     3301 gcccgggtca cgtacaaatt ttcaaccagg ggctgaagtc ttatcgcgat ctgccgctgc
     3361 gtatggccga gtttggtagc tgccaccgta acgagccgtc aggttctctg catggcctga
     3421 tgcgcgtgcg tggattcacc caggatgacg cgcatatctt ctgtactgaa gaacaaattc
     3481 gcgatgaagt taacggatgt atccgtttag tctatgatat gtacagcact tttggcttcg
     3541 agaagatcgt cgtcaaactc tccactcgtc ctgaaaagcg tattggcagc gacgaaatgt
     3601 gggatcgtgc tgaggcggac ctggcggttg cgctggaaga aaacaacatc ccgtttgaat
     3661 atcaactggg tgaaggcgct ttctacggtc cgaaaattga atttaccctg tatgactgcc
     3721 tcgatcgtgc atggcagtgc ggtacagtac agctggactt ctccttgccg tctcgtctga
     3781 gcgcctccta tgtgggcgaa gacaacgagc gtaaggtacc ggtaatgatt caccgcgcaa
     3841 ttctggggtc gatggaacgt ttcatcggta tcctgaccga agaattcgct ggtttcttcc
     3901 cgacctggct tgcgccagtt caggttgtta tcatgaatat taccgattca cagtctgaat
     3961 acgttaacga attgacgcaa aaactatcaa atgcgggcat tcgtgttaaa gcagacttga
     4021 gaaatgagaa gattggcttt aaaatccgcg agcacacttt gcgtcgcgtc ccgtatatgc
     4081 tggtctgtgg tgataaagag gtggaatcag gcaaaattgc cgttcgcacc cgccgtggta
     4141 aagacctggg aagcatggac gtaaatgaag tgatcgagaa gctgcaacaa gagattcgca
     4201 gccgcagtct taaacaattg gaggaataag gtattaaagg cggaaaacga gttcaaacgg
     4261 cgcgccctaa ccgtatcaat ggcgaaattc gcgcccagga agttcgctta acaggtctgg
     4321 aaggcgagca gcttggtatt gtgagtctga gagaagctct ggagaaagca gaagaagccg
     4381 gagtagactt agtcgagatc agccctaacg ccgagccgcc ggtttgtcgt ataatggatt
     4441 acggcaaatt cctctatgaa aagagcaagt cttctaagga acagaagaaa aagcaaaaag
     4501 ttatccaggt taaggaaatt aaattccgtc ctggtacaga tgaaggcgac tatcaggtaa
     4561 aactccgcag cctgattcgc tttctcgaag agggtgataa agccaaaatc acgctgcgtt
     4621 tccgcggtcg tgagatggcg caccagcaaa tcggtatgga agtgcttaat cgcgtgaaag
     4681 acgatttgca agaactggca gtggtcgaat ccttcccaac gaagatcgaa ggccgccaga
     4741 tgatcatggt gctcgctcct aagaagaaac agtaaggcct tcaagtagca ctgtctgtgg
     4801 agccttcggg cttcacaggc tttgttcgcc tttgtttcgt ttattaacaa tgcgaagtgg
     4861 aagttattaa aatgccaaaa attaagaccg tacgcggtgc tgctaagcgc ttcaaaaaaa
     4921 ccggtaaagg tggttttaag cacaagcacg ctaacctgcg tcacattctg accaaaaaag
     4981 cgaccaaacg taaacgtcac ctgcgtccga aagccatggt ttccaaaggc gatctgggcc
     5041 tggtaatcgc gtgcctgccg tacgcataag ccgttaacgt ttttaacttt ttaattagaa
     5101 tatagataca ggagagcaca tatggctcgc gtaaaacgtg gtgttattgc acgcgcacgt
     5161 cacaagaaaa ttttgaaaca agctaaaggc tactacggtg cgcgttctcg cgtataccgc
     5221 gttgccttcc aggctgttat caaagctggt cagtatgctt accgtgaccg tcgtcaacgt
     5281 aagcgtcagt tccgtcaact gtggattgcg cgtatcaacg cagcagcacg tcagaacggt
     5341 atttcttaca gcaaattcat caatggcctg aaaaaagcct ctgttgaaat cgaccgtaag
     5401 atcctggctg atatcgcagt attcgacaaa gtagcgttca ccgctctggt tgaaaaagcg
     5461 aaagcagctc tggcataagc cagttgaaag agggagctag tctccctctt ttcgtttcaa
     5521 cgccatcaaa acattgactt ttatcgccgt agccttttca ataaaggtct tttgaagagt
     5581 accaaaaggt aacgcaagca atgaatgctg ctattttccg cttctttttt tactttagca
     5641 cctgaatcca ggaggctagc gcgtgagaag agaaacggaa aacagcgcct gaaagcctcc
     5701 cagtggaggc tttttttgta tgcgcgtttt gaaaaataaa aagccagtct atcaggcgta
     5761 attgtcgcag ccagtctgga tacggccagt gcggagaaac cggagcgtac ttaagtacgt
     5821 gaggatttcg agcactgccc gggaccaaaa tggcaagtaa aatagcctga tgggataggc
     5881 tctaagtcca acgaaccagt gtcaccactg acacaatgag gaaaaccatg tcacatctcg
     5941 cagaactggt tgccagtgcg aaggcggcca ttagccaggc gtcagatgtt gccgcgttag
     6001 acaatgtgcg cgtcgaatat ttgggtaaaa aagggcactt aacccttcag atgacgaccc
     6061 tgcgtgagct gccgccagaa gagcgtccgg cagccggtgc ggttatcaac gaagcgaaag
     6121 agcaggttca gcaggcgctg aatgcgcgta aagcggaact ggaaagcgct gcactgaatg
     6181 cgcgtctggc ggcggaaacg attgatgtct ctctgccagg tcgtcgcatt gaaaacggcg
     6241 gtctgcatcc ggttacccgt accatcgacc gtatcgaaag tttcttcggt gagcttggct
     6301 ttaccgtggc aaccgggccg gaaatcgaag acgattatca taacttcgat gctctgaaca
     6361 ttcctggtca ccacccggcg cgcgctgacc acgacacttt ctggtttgac gctacccgcc
     6421 tgctgcgtac ccagacctct ggcgtacaga tccgcaccat gaaagcccag cagccaccga
     6481 ttcgtatcat cgcgcctggc cgtgtttatc gtaacgacta cgaccagact cacacgccga
     6541 tgttccatca gatggaaggt ctgattgttg ataccaacat cagctttacc aacctgaaag
     6601 gcacgctgca cgacttcctg cgtaacttct ttgaggaaga tttgcagatt cgcttccgtc
     6661 cttcctactt cccgtttacc gaaccttctg cagaagtgga tgtcatgggt aaaaacggta
     6721 aatggctgga agtactgggc tgcgggatgg tgcatccgaa cgtgctgcgt aacgttggca
     6781 tcgacccgga agtttactct ggtttcgcct tcgggatggg gatggagcgt ctgactatgt
     6841 tgcgttacgg cgtcaccgac ctgcgttcat tcttcgaaaa cgatctgcgt ttcctcaaac
     6901 agtttaaata aggcaggaat agattatgaa attcagtgaa ctgtggttac gcgaatgggt
     6961 gaacccggcg attgatagcg atgcgctggc gaatcaaatc actatggcgg gcctggaagt
     7021 tgacggtgta gaaccggttg ccggtagctt ccacggcgtg gtcgttggtg aagtggttga
     7081 gtgtgcgcag catccgaacg ctgacaaact gcgtgtgaca aaagtgaatg tcggcggcga
     7141 tcgcctgctg gacatcgtct gcggtgcgcc aaactgccgt cagggcctgc gtgtggcggt
     7201 agcgaccatt ggtgctgttc tgccgggtga tttcaaaatt aaagcggcga aactgcgcgg
     7261 cgaaccgtct gaagggatgc tgtgctcctt ttctgagctg ggaatttctg acgaccataa
     7321 cggcattatc gaactgcctg cagatgcgcc gattggcact gacatccgcg aatacctgaa
     7381 actcgatgac aacaccatcg aaatcagcgt aacgccaaac cgtgccgact gtttaggtat
     7441 cattggtgtt gcgcgtgacg ttgccgtgct gaatcagctg ccgctggttg aaccggaaat
     7501 cgttccggtt ggtgcgacca tcgacgacac gctgccgatt acagtcgaag cgccggaagc
     7561 ctgcccgcgt tatcttggcc gtgtggtaaa aggcattaac gttaaagcgc caactccgct
     7621 gtggatgaaa gaaaaactgc gtcgttgcgg gatccgttct atcgatgcag ttgttgacgt
     7681 caccaactat gtgctgctcg aattgggcca gccgatgcac gctttcgata aagatcgcat
     7741 tgaaggcggc attgtggtgc ggatggcgaa agagggcgaa acgctggtgc tgctcgacgg
     7801 tactgaagcg aagctgaatg ctgacactct ggtcatcgcc gaccacaaca aggcgctggc
     7861 gatgggcggc atcttcggtg gcgaacactc tggcgtgaat gacgaaacac aaaacgtgct
     7921 gctggaatgc gctttcttta gcccgctgtc tatcaccggt cgtgctcgtc gtcatggcct
     7981 gcatactgat gcgtctcacc gttatgagcg tggcgttgat ccggcactgc agtacaaagc
     8041 gatggaacgt gcgacccgtc tgctgattga catctgcggt ggtgaggctg gtccggtaat
     8101 tgatatcacc aacgaagcaa cgctgccgaa gcgtgcaacc atcactttac gtcgtagcaa
     8161 actggatcgc ctgatcggcc atcatattgc ggatgagcag gtaactgaca ttctgcgtcg
     8221 tctcggctgc gaagtgaccg aaggcaaaga cgagtggcag gcagttgcgc cgagctggcg
     8281 tttcgacatg gagattgaag aagatctggt cgaagaagtc gcgcgtgttt acggctacaa
     8341 caacatcccg gatgagccgg tacaggcaag cctgattatg ggtactcacc gtgaagctga
     8401 cctgtcgctc aagcgcgtga aaacgctgct caacgataaa ggctatcagg aagtgatcac
     8461 ctatagcttc gttgatccga aagtgcagca gatgatccat ccaggcgttg aagccttact
     8521 gctgccaagc ccgatctctg ttgaaatgtc agcaatgcgt ctttctctgt ggaccggcct
     8581 gctggcaacc gtggtgtaca accagaaccg tcagcagaac cgtgtgcgca ttttcgaaag
     8641 cggtctgcgt ttcgtaccag atactcaggc accgttgggc attcgtcagg atctgatgtt
     8701 agccggtgtg atttgcggta accgttacga agagcactgg aacctggcaa aagagaccgt
     8761 tgatttctat gatttgaaag gcgatcttga atccgttctc gacctgaccg gtaaactgaa
     8821 tgaggttgag ttccgtgcag aagcgaatcc ggcactgcat ccggggcaat ccgcagcgat
     8881 ttatctgaaa ggtgaacgta ttggttttgt tggggttgtt catcctgaac tggaacgtaa
     8941 actggatctt aacggtcgca ctctggtgtt cgaactggag tggaacaagc tcgcagaccg
     9001 cgtggtgcct caggcgcgcg agatttctcg cttcccggcg aaccgtcgtg acatcgcggt
     9061 ggtggtcgca gaaaacgttc ccgcagcgga tattttatcc gaatgtaaga aagttggcgt
     9121 aaatcaggta gttggcgtaa acttatttga cgtgtaccgc ggtaagggtg ttgcggaggg
     9181 gtataagagc ctcgccataa gcctgatcct gcaagatacc agccgtacac tcgaagaaga
     9241 ggagattgcc gctaccgtcg ccaaatgtgt agaggcatta aaagagcgat tccaggcatc
     9301 attgagggat tgaacctatg gcgcttacaa aagctgaaat gtcagaatat ctgtttgata
     9361 agcttgggct tagcaagcgg gatgccaaag aactggttga actgtttttc gaagagatcc
     9421 gtcgcgctct ggaaaacggc gaacaggtga aactctctgg ttttggtaac ttcgatctgc
     9481 gtgataagaa tcaacgcccg ggacgtaacc cgaaaacggg cgaggatatt cccattacag
     9541 cacggcgcgt ggtgaccttc agacccgggc agaagttaaa aagccgggtc gaaaacgctt
     9601 cgcccaaaga cgagtaatct gatctaacta aaaaggccgc tctgcggcct tttttctttt
     9661 cactgtcgaa gagtcaccgt aaaatcaacg ccatgacact tcagcagaac ggataccatg
     9721 ctgacacttg cccgccaaca acagcgacaa aatattcgct ggttattatg cctgtcagtt
     9781 ttgatgctgc tggcgcttct cttaagcctt tgcgccggtg aacaatggat ctcgccaggt
     9841 gactggtttt ctcctcgtgg cgaactgttc gtctggcaaa ttcgcctgcc acgtacgctg
     9901 gctgtattgc tggttggtgc ggcgctggct atatccggcg ctgtaatgca ggcgttgttt
     9961 gaaaatcctc tggcagaacc tggactactt ggcgtctcta acggcgcagg cgtggggctt
    10021 atcgccgcgg tattgcttgg gcaagggcaa ctccccaact gggcgctagg gctgtgtgcg
    10081 attgctgggg cgcttatcat cactttaata ctcttacgtt tcgcccgtcg tcatctttcg
    10141 accagtcggt tattgctggc tggcgttgca ttagggatta tctgtagcgc actaatgacg
    10201 tgggctatct acttttccac ctctgttgat ttacgtcagc tgatgtactg gatgatgggc
    10261 ggttttggcg gcgtagactg gcggcaaagc tggctgatgc tggcattgat ccccatgttg
    10321 ttgtggatct gttgtcagtc caggccgatg aatatgttag cacttggcga gatctcggcg
    10381 cggcaactgg gtttacccct gtggttctgg cgcaatgtgc tggtggcagc gaccggctgg
    10441 atggttggcg tcagtgtagc gctggcgggt gctatcggct ttattggtct ggtgatccca
    10501 catattcttc ggttgtgtgg tttaaccgat catcgcgcat tacttcccgg ctgcgcgctg
    10561 gcaggggcga gcgcattgct gctggccgat attgtagcgc gcctggcatt agctgccgca
    10621 gagctgccta ttggcgtggt caccgcaacg ttgggtgcgc cggtgtttat ctggttattg
    10681 ttaaaagcag gacgttagcc gcaaaaagac ggtctatgat taaaagctaa gatttttact
    10741 gaccacaccc aggagaaacg atgcaagatt ccattctgac gaccgtagtg aaagatatcg
    10801 acggtgaagt gaccacgctg gagaagtacg ccggtaatgt gctgttgatt gtcaatgtcg
    10861 cctcaaagtg tggcttaacg ccgcaatatg agcagttgga gaatattcag aaagcctggg
    10921 ccgatcgagg ttttgtggtg ctgggattcc cgtgcaacca gtttctggaa caagaaccgg
    10981 gcagcgatga agagattaaa acttactgta ccaccacatg gggggtgacg ttcccgatgt
    11041 tcagtaagat tgaagttaat ggcgaaggac gccatccgct gtatcaaaaa ttgatttccg
    11101 cagcgccgac cgcagtcgcg ccggaagaga gcggattcta tgcccgtatg gtcagcaaag
    11161 gtcgtgcacc gctgtaccct gatgatattt tatggaattt tgagaaattt ttagttggca
    11221 gggacggatt ggtcatccag cgtttttccc cggatatgac gccggaagat cccattgtga
    11281 tggaaagcat taaactggcg ttggcaaaat aatgtctatt gtgatgcagt tacaggatgt
    11341 tgcggaatct acccgcctgg ggccgctttc tggcgaggtt cgggctgggg agatcctgca
    11401 cctggtgggg ccgaatggcg cgggtaagag taccttactg gcgcgaatgg ccggaatgac
    11461 cagcggtaag ggaagcattc agttcgcggg gcaaccactg gaagcatggt ccgcaacaaa
    11521 actcgcgctg catcgtgcct atctttcaca acagcagacg ccgccgtttg caatgccggt
    11581 ctggcactac ctgacactgc atcagcacga taaaacgcgt accgaactac tgaatgatgt
    11641 cgcaggggcg ctggctcttg atgacaaact cggtcgtagc accaatcaac tttccggcgg
    11701 tgaatggcaa cgcgtacgtc ttgctgcggt ggtgctgcaa atcacaccac aagccaatcc
    11761 cgcaggccaa ttgctgcttc ttgatgagcc gatgaacagt cttgatgttg cgcaacaaag
    11821 tgcgttagac aaaattctga gcgcgctgtg tcagcaagga ctggcgattg tgatgagcag
    11881 tcacgatctc aaccacacat tgcgtcatgc gcatcgggcg tggttgctaa aaggtggaaa
    11941 aatgctggcc agtggacgca gagaagatgt gctcacgccg ccaaatctgg cgcaggccta
    12001 tgggatgaat tttcgccgtc tggatatcga aggtcacaga atgctgattt cgaccatctg
    12061 atttcattag tatcttgaaa atcgggtaat tacgacgcta aattaatgca agaataaaaa
    12121 acagaggaac cgtacggaat gcgtttctgc cttattttga tcacagcact gtttctggcc
    12181 gggtgtagcc accataaagc accgccgcca aatgccagac tttctgattc gattaccgtt
    12241 attgccggtt tgaacgacca gctacaaagc tggcatggca cgccgtatcg ttatggtggc
    12301 atgacgcggc gcggtgtgga ctgttcggga tttgtggttg tgacgatgcg cgatcgtttc
    12361 gatttgcagc tgccccgaga aaccaaagaa caagcctcta tcggcacgca aattgataaa
    12421 gacgagttgc tgcctggtga cctggtcttt ttcaaaacgg gttccggaca aaatggtttg
    12481 catgtaggta tttacgatac caacaaccaa tttatccacg cctctaccag caagggagtg
    12541 atgcgttcct cacttgataa tgtctattgg cagaaaaatt tctggcaggc gagacgaatc
    12601 tagtatgtcg aaaattggac gtcgatgata catcgccgtc catttactcc agaatacagt
    12661 agtattttgc agacaaaaat tccgtaaatg gattattcgt ttataatcag gataatctaa
    12721 aaaaaggacc cctgaaaaag tggttaacat ggaacaaatg aaagttactc cttctgtaat
    12781 tgttatgtct tatggcctca tcccttttag ccggatactg aaaaacatcc ttcgagaggg
    12841 acggttacca tgaagatttt tttggagaat ctttatcatt ctgattgcta tttcctgccg
    12901 ataagagata accagcaagt ccttgtcggc gttgagttaa ttacccattt ctccagtgaa
    12961 gatggcacgg tgcgtatccc gacgagtcgg gttatagcgc aacttaccga ggagcaacat
    13021 tggcaattat tttcagagca gttggaatta ctaaaatcat gccaacattt ttttattcaa
    13081 cataaactct ttgcctggct taatttaacg ccacaagtgg ctacattatt attagagagg
    13141 gataattacg caggcgaact attaaaatat ccgtttattg aactgttgat taatgagaat
    13201 tacccgcatc tcaatgaagg aaaagataat cgcggtcttt tatccttatc gcaagtgtat
    13261 ccccttgtgt taggtaattt gggagcaggc aacagcacaa tgaaagcggt ttttgatggt
    13321 cttttcaccc gcgtaatgct ggataaaagt tttattcagc aacaaatcac gcatcgctcc
    13381 ttcgaaccgt ttattcgtgc gattcaggcg caaatttccc cttgttgcaa ttgcatcatc
    13441 gcggggggca ttgatacgcc agaaatatta gcacagatta cccccttcga tttccatgct
    13501 ctccagggat gcctgtggcc tgccgttccc ataaatcaga taacgacgtt ggttcagcga
    13561 taaccctcct gtttgctggt gtttaagacg agagtaaccg tctacactat caaacaggag
    13621 gctctatgac cctgtctttt attacccgct ggcgcgatga attgccagaa acctatacag
    13681 cactttcccc tacgccttta aataatgccc ggctgatttg gcataatacc gaactcgcta
    13741 acacgctgag tattccatcg tcgctgttta aaaatggcgc aggtgtctgg ggcggcgaaa
    13801 ccctactgcc tggtatgtca ccactggcgc aggtttacag tggtcatcag ttcggcgtct
    13861 gggcgggcca actgggtgat gggcgcggca ttttacttgg cgaacaactg cttgctgatg
    13921 gcactacaat ggactggcat ctgaaaggtg ctggcctgac gccttattcg cgaatgggtg
    13981 atggacgggc ggtattacgt tcgacgatac gagaaagtct cgccagtgag gcgatgcatt
    14041 atctgggcat tccgacgacc cgcgcgttaa gtatcgtcac cagcgattcg ccagtgtatc
    14101 gggaaacggt ggagccaggc gcgatgctga tgcgtgtggc accaagtcat ctgcgctttg
    14161 gtcatttcga acatttttac tatcgccgcg agccggataa ggttcgtcag ttggctgact
    14221 ttgccattcg tcattactgg tcacatcttg aagatgatga ggacaaatac cgtctctggt
    14281 ttaacgatgt tgtcgcacgt accgcatcgt taattgccca atggcagacg gtcggctttg
    14341 cacatggggt gatgaatacc gacaacatgt cgctgctggg actgacgctt gattacgggc
    14401 cgtttggttt tcttaatgat tacgaacccg gttttatttg taatcactcg gatcatcaag
    14461 gacgttacag ctttgacaat caacctgctg tcgcgttgtg gattttacag cgtctggcgc
    14521 agacattgtc accatttgtt gccgtagatg ccctgaatga ggccctggac agctatcagc
    14581 aggttttgtt gacgcattat ggacaacgga tgcggcagaa actgggcttt atgacggagc
    14641 aaaaagagga taacgcgcta ctgaatgaat tattcagtct gatggcgcga gagcgcagcg
    14701 attatacccg cacatttcgc atgctgagtc tgaccgagca gcacagcgcg gcgtcaccgc
    14761 tacgtgatga gtttattgat cgtgcggcat ttgatgactg gtttgcccgt tatcgagggc
    14821 gtttgcaaca agacgaggtt agcgatagtg agcgtcagca actgatgcaa agcgttaacc
    14881 ccgctctggt attgcgcaac tggttggcgc aacgggcgat tgaagcggca gaaaagggtg
    14941 atatgacgga attgcaccgc ctgcatgagg cgttgcgaaa tcctttcagc gacagagatg
    15001 atgactatgt cagtcgtcca cctgactggg gtaaacggct ggaagtcagc tgctcgagtt
    15061 aaaggctact tggtcaacag cagcttgcca gcctgagttt tacggagcag atattcttgc
    15121 ccgtcatgat cgataatcag tttgccatcc ggacctaaca gtgtctggct ggaaattcgc
    15181 cgaataggct gcggggaagc ggtcttgtga ttggcatccg tttcaggcgt gagttttctg
    15241 ctatccgtat aacgcataaa aattaataat aattgatata acaatgattc ttattatcaa
    15301 tattatggct gaccagagca agcagtaatg tgttgagaag acaacaagtt gcgcagaaat
    15361 gcccatccca tcaggaatgg gcacgcattc agaagcgggt atctaccgca gaggctagtt
    15421 tttcgaccag gcgttcggta tcttcccagc ccagacacgg gtcggtaatg gattgaccgt
    15481 aagtgagcgg ctgaccgccg acgatttttt gcgttccttc gcgcaggaaa ctttccgcca
    15541 taattccagc aatcgccgta gagccattgc ggatttgctg acaaatatcc tcacaaactt
    15601 ctaactggcg acggtgctgc ttctggcagt taccgtggct gaaatccacc accagatgtt
    15661 caggtaaatc aaactcgtgc agcgtatcgc aggctgcggc gatatcatcg gcatgataat
    15721 tcggtttttt gccgccacgc ataataatgt ggccatacgg gttgccgctg gtctgataga
    15781 tggtcatctg accatttttg tctggcgaga ggaacatatg gctggcgcgg gctgcgcgga
    15841 tagcatccac agcaatccgc gtattgccat cggtaccatt tttaaaacct accggacagg
    15901 agagtgccga agccatttca cggtggatct gactttcggt agtacgtgcg ccaatcgcgc
    15961 cccaactgat taaatcagca ataaactgac cggtcaccat atcgaggaac tcagtcgcgg
    16021 ttgggacgcc cagctcattt acctgtaaaa gtaatttgcg cgccagctcc agaccgtgat
    16081 ttatccgata gctgccgttt aaatctggat cggagattag tcctttccag ccgacaacag
    16141 ttcgtggttt ttcaaaatag gtgcgcatta cgatttccag ccgtgactgg tactggttgc
    16201 gcagcgactg cagacgggtg gcgtactcca ttgcagcggt gagatcgtgg atcgagcagg
    16261 ggccaataat gaccaacagt cgcttatctt caccattgag tattttttca attctgcggc
    16321 gggagtcggt gacatgggtg gcgacgccag gcgttacggg ataccgtagc gcgagttcgg
    16381 cgggcgttac caggctctca atacgcgcag tacggagttc gtcagttctg ttcattacaa
    16441 gtctcagaaa tttgttgctt ctacggcagg actgccggga agtgatgcgc atcacgataa
    16501 accaatcgct gctgatttca agtgcgaggt gtgtcacacc tcgccgggtg gttagcatga
    16561 taacaaaaaa ataagctaat gcactagttc tctagtacat tcggcgacta aggcccatga
    16621 tatcgaggat cttggtggca atctcttcta ccgaataatt ggtactgtta atccacggga
    16681 tctgattttt acggtacaag gcttccactt ccgcgacttc catcctgcac tgacgaagcg
    16741 aggcatagcg actgttctcc cgacgttcct cgcgaatcgc cgccagacgt tccgggtcga
    16801 tagtcaggcc gaacaattta tgctgaagcg gtttgagcga cgcgggtagc accagattat
    16861 ccatatcgtc ggcaataaag gggtagtttg ccgcgcggat accaaattgc attgccagat
    16921 acagactggt gggggtttta ccacagcgag aaacaccgag caggatcacc tgagcctgat
    16981 ccagattgcg caacgaaatg ccgtcatcgt gggcgagggt gtaatcaatc gccgcaatgc
    17041 gcgcatcata tttattgaga ttattagggt taaggccatg ggtacgatga gcaatcggcg
    17101 ttggatccag tttcatctct tgttgtagcg gggcaaccag cgcctgaacg atatcctggc
    17161 aaaaaccttc actttgcaag atgatggcgc gaatttccgg caacacgatg gagtagaaga
    17221 ccagcggacg cacgcctgtc tggtgataaa ttgcgtcaat ctgatccttc actgcccgtg
    17281 cacggctctc attttcgaca aacggcagcg tgatgctgct gatagtgacg ggaaattgtg
    17341 acattactgc gtgtcctaat acctccgcag ttattgccgt accatcagaa atataaaaaa
    17401 cgtggcgatc aacagcatta tccattttgt tcttcccgtg atgcagacat aattctatga
    17461 aagcataaat taaaaacgca cagaagcgta gaacgttatg tctggtttat aaaatgaacc
    17521 ttcaatttta ttttttatga aaacagcatt tcatttttat ggtttcgttt ataccgatgg
    17581 tttatgtgga aattgtcgaa aagagcagat ttgcgcaacg ctgggatcag tcttaacaag
    17641 taaaaaaata tatttgcttg aacgattcac cgtttttttc atccggttaa atatgcaaag
    17701 ataaatgcgc agaaatgtgt ttctcaaacc gttcatttat cacaaaagga ttgttcgatg
    17761 tccaacaatg gctcgtcacc gctggtgctt tggtataacc aactcggcat gaaggatgta
    17821 gacagggttg ggggcaaaaa tgcctccctg ggtgaaatga ttactaacct ttccggaatg
    17881 ggtgtttccg ttccgaatgg tttcgccaca accgccgacg cgtttaacca gtttctggac
    17941 caaagcggcg taaaccagcg catttatgaa ctgctggata aaacggatat tgacgatgtt
    18001 actcagcttg cgaaagcggg cgcgcaaatc cgccagtgga ttatcgacac tcccttccag
    18061 cctgagctgg aaaacgccat ccgcgaagcc tatgcacagc tttccgccga tgacgaaaac
    18121 gcctcttttg cggtgcgctc ctccgccacc gcagaagata tgccggacgc ttcttttgcc
    18181 ggtcagcagg aaaccttcct caacgttcag ggttttgacg ccgttctcgt ggcagtgaaa
    18241 catgtatttg cttctctgtt taacgatcgc gccatctctt atcgtgtgca ccagggttac
    18301 gatcaccgtg gtgtggcgct ctccgccggt gttcaacgga tggtgcgctc tgacctcgca
    18361 tcttctggcg tgatgttctc cattgatacc gaatccggct ttgaccaggt ggtgtttatc
    18421 acttccgcat ggggccttgg tgagatggtc gtgcagggtg cggttaaccc ggatgagttt
    18481 tacgtgcata aaccgacact ggcggcgaat cgcccggcta tcgtgcgccg caccatgggg
    18541 tcgaaaaaaa tccgcatggt ttacgcgccg acccaggagc atggcaagca ggttaaaatc
    18601 gaagacgtac cgcaggaaca gcgtgacatc ttctcgctga ccaacgaaga agtgcaggaa
    18661 ctggcaaaac aggccgtaca aattgagaaa cactacggtc gcccgatgga tattgagtgg
    18721 gcgaaagatg gccacactgg taaactgttc attgtgcagg cgcgtccgga aaccgtgcgc
    18781 tcacgcggtc aggtcatgga gcgttatacg ctgcattcac agggtaagat tatcgccgaa
    18841 ggccgtgcta tcggtcatcg catcggtgcg ggtccggtga aagtcatcca tgatatcagc
    18901 gaaatgaacc gcatcgaacc tggtgacgtg ctggtcactg acatgaccga cccggactgg
    18961 gaaccgatca tgaagaaagc atctgccatc gtcaccaacc gtggcggtcg tacctgtcac
    19021 gcggcgatca tcgctcgtga actgggcatt ccggcggtag tgggctgtgg tgatgcaaca
    19081 gaacggatga aagacggtga gaacgtcact gtttcttgtg ccgaaggtga taccggttac
    19141 gtctatgcgg agttgctgga atttagcgtg aaaagctcca gcgtagaaac gatgccggat
    19201 ctgccgttga aagtgatgat gaacgtcggt aacccggacc gtgctttcga cttcgcctgc
    19261 ctgccgaacg aaggcgtggg ccttgcacgt ctggaattta tcatcaaccg tatgattggc
    19321 gtccacccac gcgcactgct tgagtttgac gatcaggaac cgcagttgca aaacgaaatc
    19381 cgcgagatga tgaaaggttt tgattctccg cgtgaatttt acgttggtcg tctgactgaa
    19441 gggatcgcga cgctgggtgc cgcgttttat ccgaagcgcg tcattgtccg tctctctgat
    19501 tttaaatcga acgaatatgc caacctggtc ggtggtgagc gttatgagcc agatgaagag
    19561 aacccgatgc tcggcttccg tggcgcggga cgctatgttt ccgacagctt ccgcgactgt
    19621 ttcgcgctgg agtgtgaagc agtgaaacgt gtgcgcaacg acatggggct gaccaacgtt
    19681 gagatcatga tcccgttcgt gcgtaccgta gatcaggcga aagcggtggt tgaggaactg
    19741 gcgcgtcagg ggctgaagcg tggtgagaac gggctgaaaa tcatcatgat gtgtgaaatc
    19801 ccgtccaacg ccttgctggc cgagcagttc ctcgaatatt tcgacggctt ctcaattggc
    19861 tcaaacgaca tgacgcagct ggcgctcggt ctggatcgtg actccggcgt ggtgtctgaa
    19921 ctgttcgatg agcgcaacga tgcggtgaaa gcactgctgt cgatggcgat tcgtgccgcg
    19981 aagaaacagg gcaaatatgt cgggatttgc ggtcagggtc cgtccgacca cgaagacttt
    20041 gccgcatggt tgatggaaga ggggatcgat agcctgtctc tgaacccgga caccgtggtg
    20101 caaacctggt taagcctggc tgaactgaag aaataaaata aatccccggc ggcgttttgt
    20161 cgccgggttt atgtgatccc cgaagatgaa acttattcaa tctcttcaca gacatcctgc
    20221 gttaatcgcc gcataatatc tttccgtaac aaaaactttt gtattttacc tgaggcagtg
    20281 cgcggtagtt tttcgattac cacgatatgt tcaggatatt tatattttgc gacccgttta
    20341 cggctaaaaa aagccaccac ctcttccagc gataatgaat gatgcggcgc tttcagcacg
    20401 acataagcgc atgatcgttc acctaaacgt tcatcgggca ttgcaaccac acaggcatcg
    20461 tgaattttag gatgctgcaa taaaatatct tccacttcac ggctgctaat attttcgccg
    20521 ccgcggacaa taatatcttt tttgcgtccg gtaattttta tatagccagc ctcatccata
    20581 cggcagagat cgccgctgta ataccagcct tcttcatcca gggcacgggc ggttaattca
    20641 ggttcatcaa aatatcccat aaacacattg gggccacgcg aggcttcttc accttcgcaa
    20701 cctggcggta aggtcttgcg tgcgtcatcg accactttaa tctctacacc tgcggcagcg
    20761 taaccatcgg tgtgcataaa gcgcgacaaa ggatcatcga gattcaccac cgcatgcggc
    20821 gaactttctg tggaaccata aacacttaat aatttaatgc cgcgctgctg gcattcacgc
    20881 gccacttttt tggggattgt ggtaccgccg caaagaaaga agcgcagcgc tgaaaggtct
    20941 gcgggttgtt tctctagtaa attcaaaaga tcatagacaa acggcgttgc gccgagcata
    21001 caggagcaac gctgctgctc aagcagcgcg agacacgcat caggagtgaa aatatctaac
    21061 aacacgctgc gagcgccaat taagaatggt gccgttacgc catgcagaaa gcccgttgcg
    21121 tgaccaagtg gcgcaggcat cataaagaca tcctgccagg ttagattcag ccgcgcgcaa
    21181 taagcccgct cactggcgag aatattgtta tgcgtaagca tcacgccctt tggcagaccc
    21241 tcggttccgg aggtaaacag caccgcagct aattcatcgc cgtgggtcgt tatcgccgtg
    21301 gtcagtgggg tattgtcggc gataatctga cttaatgaga gggaagaggt ggcgggagcc
    21361 agtttgtcca cgccgacaat ttgttgtagt tgtggaagct gattttgcag cggcaggatt
    21421 aaatctaccg gacgcgtttg tttaaacaac gtcggtgtaa agaacatttt tgcctgacac
    21481 ttattaagca cccataccag ttctgcttcc cgccaggaag gcaacagcgg tacggaaacc
    21541 gcaccgattt tcaggcaggc aagatagata acggtaaatt cacaccagcc tggcagttga
    21601 aatgctatgc gatcgcctga ttcaataccc ttcgctaaca tccagtttgc cagacagctc
    21661 gcggcgtgat cgagcgcgct ataggtgtac gatgctccat gattatcgac cacggcaatt
    21721 ttgtctggca tcgcacgagc ggtctgctgc cagtaatcgg ccagcgaagc atcgccccat
    21781 agcccttgct gacgatacgc cgcacgacgt tgttcgttaa acgttaatgt cactttcatt
    21841 ttggactctc gaaacagaac atcaggcccg agatgcgggc ctgtgggatg catgattagc
    21901 cgtagcgaaa atcaataccg aaggtgcctg cgggatattg ccactgttcg agaatagtgt
    21961 taccgcacag cacccgacag gtgccgcact ccagacaacc ggcggaatca aaatgaatgt
    22021 ttcctgcgtc atcctgctta taaagtccgg cagggcaggc tttcattaat ttacggaatt
    22081 cattgatatc gggatttgcc gccaaaatga tatgcgggtg gccctcatca acatggaatt
    22141 tattgacgcc taatttgatg tcgacgttaa ccgtagcgtt ctggctcata gcgcggttgc
    22201 tcccttaatg ccatctttca gcaagttgat cagcccaatt ttcttcgcgt gtcccatgat
    22261 cattttgcgt accggctggt taggcttacc gtcaatggtg aacatctcgt tcatgatgtc
    22321 ggctaccatt cgtgggtatt ggctaaacag gcgcgggttt tccatcagcg ccgggatctt
    22381 gcgaaaatgc tgcatatcac gcatgacgca gctttgttcc agctcacgtt tgtattgcgc
    22441 cagactgctg gcggagaaat ccgcgcgttc tttggcggcg atcactgttg tggcggcagc
    22501 ctgagccgat gcaatggcta aatccatgcc gcggaccgta aaacccaaat tcaggcagaa
    22561 gcctgcggcg tcaccaacga tcatcacgcc atcgttaacc agttgcggta ccatcgccag
    22621 accgccttct ggcaccatat gcgcggaata ttcaagcagt ttgccgccgc taatcagcgg
    22681 gcgaatggcg gggtgttgtt taaaatcttc cagcatttgc ggcacgcttt tttgcgcatg
    22741 ggcgatatca gccaatccac aaaccagccc caaggatatg gaatccttgt tggtatagag
    22801 aaatcccccg cccatcaggc cgtcagaagg ggaaccggca aacagccagg cggcaccttc
    22861 ctcgcccgta atattaaagc gatcgttgat ctgttctggt gtgaggccaa taacctcttt
    22921 aacaccaaca gcgtaatgat gcggatcgga agcgggaacc attcccagcg agcggccaag
    22981 catcgagtta acgccatcgg ctaaaatcac cacattggct tcgagaatat catccccggc
    23041 ctggactcca gtgaccttgt ttccttcacg aaccaacgca tcgacgcgaa ctcccgggat
    23101 aaactgtgcg cccgcctgct cggcttgttc catcaaccac gggtccagac gattacgcaa
    23161 tacggtataa gatgcgtgtt gcggaacatc tggttgctcg cggtgaaaat cgagggtaac
    23221 ggcgctctct tcggttaaga aggagatttt ctcgcgtgtg accttgcgtt ctaccggcgc
    23281 tgatgctgca aagcctggaa tgattgcttc aagtgtgtgg gcataaagac gcccgccggt
    23341 catgttttta catccggcac tgtcgccgcg ttctatcacc aggatatcca gcccggctcg
    23401 cgccatgacc agtgcggcaa cgctaccagc aacgcccgca ccgaccacaa tggcatcaaa
    23461 tttgtcatcc gacatgcgtt atgctccctt cccgctgcgt ttacagcggg agatgtggtt
    23521 gaaaggggaa ttagcggctc aactggctaa tcagggcagg gacgactttg tagatatcgc
    23581 ccaccagacc gtagtcggca tagttgaaga ttggcgcatt tttatcttta ttgatggcga
    23641 caatcacttt tgcgccgttg ccgccaacca tatgctggat ctgcccggag atccctagcg
    23701 tcaggtagag atcggatttc agcaacacgc cggagacacc gatataacgt tcacgctcca
    23761 tccagttctc gccttcggca attggacgtg aacagccgac ttcagcattc agcaccgccg
    23821 ccagttcgtg gaccattttc aggtcatcct gcgctgccag accacgaccg acgccaacca
    23881 cacgttttgc tttgctcagg tccacgctgc ttgcggcttt cgcacggcgt tcgcgacaga
    23941 gaatttcatg acgcggagca acgtaaggta ccgtttctgt cgggcactga tgagaggtat
    24001 cactggtgca cggttcctga acaccgggtg caagggtaat gatcgccagc gagctgttga
    24061 tcttttcctg agcgaacgct aacccgccat acatccggtg ttcggcgcaa atgtgaccat
    24121 cgacaatatc caccgccgtg gcatcgttca ccagcgccgc attcagttgc acacttaacc
    24181 gtgctgccag cgctttacca cgtttcgtcg cggccagtag caacatagcg gggtgtttat
    24241 ctttcagcag agcagcaatg ctttcggcgt aattttcagt gcgttgcagc gcgtcgtttt
    24301 gctcaagaac ataaatacat tttggaccat aaggcataac tgcctgcgcc tggtcggtat
    24361 tttgtacaat ggcatacacc tgttggcccc attgctgagc gccgccaaac agttcagcat
    24421 aacgttcagg attatcgcta aagacccaga cgctgtttaa ttgactcatc gtttcttctc
    24481 cgtaggcttc agttcagggc tttcttcaga tgttccgcca gctcggcaat ggcctccggc
    24541 gaatcgttat cgagaatgat gtgcttacgt tctgtttgcg gcggtacgcg aatgccggtc
    24601 agttcagcaa gtggtgcgct ctggctccag ccaatatcac ttgcctgcca ctgatttacc
    24661 ggttttttac ccgcgccgag aatggctttc atcgaaggaa tgcgtggcac gttaatatcg
    24721 gaggtgacgc agagcacggc tggaacagag agttcaataa cttcaacgtc atcttcaagc
    24781 gtgcgttcaa tcaccagtgt attgccctga cgctgaatag cactcactgc attaatcacc
    24841 ggaagttgca gaatttctcc gaccagcaag ccaacctgct gggcataaag gtcgccggaa
    24901 cctttaccaa agatcagtaa atcgaagccg atcttttcaa ctgctgccgc cagcgctttt
    24961 gcggtatcga gaggcagtgc atgctcaagt tgtgcatcct gcaccatata caggctgtgc
    25021 ggcccgcggg atagcacgtc tttgcgcact ttcgagttct gcaacaatga gccgccaacg
    25081 gtcagcgcgg ctatctcatc gtcatctgtt gcaagctggc ttgcagcttc aatggcattg
    25141 agatcgaact ggctgatttt ggcgtcggca ttgtcgaaat tcagggtgta ttctggagtg
    25201 acaacaatgt cctgttcttc aggcaccagc ttaaagcagg ttattatttt catgatttct
    25261 cctgtgagtc gcatacggaa ccatctccga tggaaggtaa aaatggcctg aaaatagtgt
    25321 gcgatagcgg tgcggggttt tcgacggagc aagctgtttt ccttgccaac aatcttgcaa
    25381 aaaaaatgcg atcgctaaaa caaaagtgca atttgcggca atatagttat tccatgaccg
    25441 tacaatcttg cattcagtgt aaataatctt gtttagctct cgctaaacgc ggttgtataa
    25501 ccgcaataca cgccgtattt tatccggcat atattgtttt tgcttgctca tggaataggg
    25561 aggttgtcat gtatcaacgc tgttttgata atgccagtga aacgctgttt gtcgctggta
    25621 aaacgccacg gctttcacgt tttgcattta gcgatgatcc aaaatgggag tctggacatc
    25681 acgttcatga caatgaaacc gagctgattt acgtcaagaa aggggtggca aggtttacca
    25741 tcgattcttc gttatatgtc gcgcatgcag atgatatagt ggtgatagaa cgcggcaggc
    25801 tgcatgcggt ggcctctgac gttaacgatc cggcaacgac gtgtacttgt gcgctgtacg
    25861 gctttcagtt tcagggggct gaggaaaatc atctactgca accgcattct tgtccggtaa
    25921 ttgccgcggg gcagggaaaa gaagtcatta aaaccttatt taatgagcta agtgtgattt
    25981 tgccgcaaag taaaaatagc caaacatctt cgttatggga tgcatttgcc tatacattag
    26041 caattcttta ctacgaaaac tttaaaaatg cttatcgttc ggagcagggg tatattaaaa
    26101 aagatgttct gataaaagat attcttttct atctgaataa taattatcgc gaaaaaatca
    26161 ctttagaaca gttatcgaaa aaatttcgtg ccagcgtcag ttatatttgc catgaattta
    26221 ccaaagagta tcgtatttcc cctattaact atgttattca acggcgtatg acggaagcaa
    26281 aatggtcact cactaatact gaattatcac aggcagagat ctcctggcgt gtgggttatg
    26341 aaaatgtcga tcactttgcc aaactgtttt tgcgccatgt cggctgttcg cccagcgatt
    26401 accgcaggca atttaaaaac tgttttgcgg aacaagaaat cctatctgaa tttcctcaac
    26461 cggtaagtct tgccggataa atctggcggc gcagtttgtt aagaagctgc gccgcctgca
    26521 gattatttgt tctgatagtc tttcaggatc tgccgacctg ctacgtaaat cataatttcg
    26581 tctgtaccgc cgccaatacg ttcacaacgg acatcacgcc agaagcggga gacgcgcgcc
    26641 tcatcggtat agcccagacc gcccatgatt tgaatcgcat catcaatgac ttccattgcg
    26701 gtacgtgcac agtacagttt tgccagcgcc gcgctggtgc gcagtgattg atgctgatcg
    26761 gcttgccatg ccactttcag caccatgttg cgcatgttgt caatcttaat cgccatcagc
    26821 gccagttttt cctggatcat ctggttatga ccaatgggtt taccaaaagc gatacgctgg
    26881 ttggcatagc gagcggcatc ttcaaaggca cactcggcaa agccggtgct gcgcgcggcg
    26941 ttgatcaggc gctccatctc aaagttgtac atcacattga ggaaacccat tccttcttcg
    27001 cccaccatgt cgctctcttc aacttccacg ttgtcgagat agacttcgca ggtgctgagc
    27061 atatgccagc cgattttatg cagcgggtta atcttaatgc cgggcttact ggagtcgacc
    27121 caccacaggg tgaaggcttt tttgggatct ttcggttgcg gatcgcgcgc cagcaccagc
    27181 atatacgggt actctttcgc accggtaata aaggttttct gtccgttgat gtaaaccttg
    27241 ccgtttttac gcgtgtaagt ggtagtggca ctgttgttat ctgagccagc acctggttcc
    27301 gtcaacgcca gggcataggc gggatcaccc gtttccaggg tgctttctgc cgttttacgt
    27361 agctgctctg cagaaccgaa acggcgcata ctgtgaatac attgaccgtt ggtaattaaa
    27421 aatgccggag caccgcattt tgacacttcc atcagcgcca gcatttgggt gacgtaatca
    27481 gcagggatac caccaaattc ttccggcacg ccaagcatgg aaataccgtt atccgccagt
    27541 gcccgcataa actcacgcgg atatgtcccg ttttgatcgc aggtgcggaa atactcttcc
    27601 ggaaagttag tcgtaatcag ttcgcgaata ctggccagca gcagttcttg ttcttcagtt
    27661 aaagaaaaat ccatcgtgat actcctgtta atgagccgct tcaggcagga caaaacccat
    27721 cgccgcatcg ataaataatc tttcgtccat cagtttgagt tctggcgaaa tcactggggt
    27781 gaaatccatt ttgtcgagaa tatctttttg taaatcgacg ccaggggcga tttcgattaa
    27841 atgcagtccg tcttctttca gcgtgaatac ggcgcgctca gtgatataac gaacatccag
    27901 ccctcgctcg agagcgattt ttccgctaaa agtaatttcc ggtagttccc gaataaattt
    27961 cttcacccgt ccttcctgga cgatattcaa tttgccatcg gcaatttctg tttttaaact
    28021 gcccgcagtt aacgtgccgc agaaaataat tttcttcgag gtggcactga tatcaataaa
    28081 tccaccggta cccatgattt tgccattgaa tttatgcacg ccgacgttac cgtgctggtc
    28141 gacttcagca aaactcaaat aacaaacatc cagaccacca ccgtgataaa aatcaaactg
    28201 ggacgtcata tccagaatgg cacgggtatt cacgttcgag ccaaaggcga taccctgtga
    28261 agtaattcca ccaatcggac ctgtttctac cgtcagaata aagtcatcag cacaaccttc
    28321 ttcccgggcg accaggccaa tgccgtcagc aataccgacg ccgacattcc ccaccgcgcc
    28381 tttacgcatt tcgaataatg cacgccgtgc aactaattta cgttggttta ggggcaggct
    28441 aagtttggtg ctgtcatcaa gggtgaagtc accagaaata aagcggttaa ccggcgcacc
    28501 gccatatagt tggctttgat ccggatcgac caccacaata tccaccagat aacccggaat
    28561 acggacagat ttaggatgca gtgtggcttt cttaaccatt ttctgcacct gcatcatcac
    28621 aataccgccg ttattgtgca ccgcctgggc aataaccaat gcgtcgagat acatcacctc
    28681 atcttcaaaa gtggcgtagc cttcactgtc gcaggtggta gcgcgaatga aagcaatatc
    28741 tggcgcaatc gctttgtaat agagatattc tttgttatca aactcgacca gtttaatcag
    28801 gtcttcttta gtgacttcat tcagtttgcc gccttgctgg cgtggatcga caaatgtccc
    28861 gatgccaata tcactaataa taccaggctg gtgggctgcg gcggcgcgta aggtttgtgt
    28921 aagtacacct tgtggatagt tataagcaat aattttattt tgttctgcga gatcagaaat
    28981 acgcggcgat tgtccccagt gaccgcataa tgcccatttc accagacctt cttgcgccag
    29041 cggactaata ccgcggtcgg cgcgatcgcc aagccctgtt ggactaataa tcgataaatt
    29101 acgtggcgtt tgagtctgtt tatatttatc agcaagagca gtaattaacg tggtggcttc
    29161 cagaataccg ccgccagcgc ctaacacaca aagtgttgct tcgtcgggaa tataattcac
    29221 cgcttcctgt gccgacagga ccggcactcg tccattaata cgaggtggtt ttacaggttt
    29281 cattgttaat tcctgattca ggtgtgattt tccctacgcc agatggataa ttagcgcaca
    29341 gagactcacg aaatattttt attaaatacg gttgaatata tttttttagt tcggcggggg
    29401 aggatattcc cgccgaaata ttattgctta tgcctgatgt aaaatagtta atactgtgcg
    29461 caaatcattt accgagattt gccctggcgc agaggctttt tttaccgcac caaaagttgc
    29521 cgccgagcca aatacttcac cagccagacg agaaattacg ccagtttttg ccattgacat
    29581 cgtgatgatt ggacgatcgg catactgctc ctgcatctcc agggtcgcgg caagcaacgc
    29641 cagcacatcg ctggtacttt gcggcatcag cgcaatctta ggaatatcgg cgtcgaagga
    29701 ctgcattttg cgcagacggg caatgatttc ttcggcttcc ggcgttttat ggaagtcatg
    29761 gttggacatg acaactttca catcatgcgc gtgggcgtag gcgacggttt ctttaacctg
    29821 atcatcgccg gtaaataact ccagatcgat catatcaacc aggccgctgt cgatggctgc
    29881 acgattgaga gcaatataag cctcggtgga aatcgcctgc tcgccgcctt ctttggcact
    29941 gcggaaggta aacagcagcg gtttttctgg catggtttca cggagaattt ttgccgccgc
    30001 catgacagac tccacattgg agaggtcggc aaagtggtcc acacgccatt ccagaatatc
    30061 aaagtccgct tcacgatagg cgagagcttc ggatttcact cgggcgatat ctttcgccat
    30121 cagcgagacg atgattttag gtgcgcccgt accaatgacg agatctttta cagttacggt
    30181 tttcattttt taccctttct gcacgcggtc agcctgtcag gcaccgaacc ccatgacctg
    30241 tttaacatat tccagaggga aatctttgcc agtccataac gtgaactgtt cagccccttg
    30301 ccacaacaac atgccgtatc catcaatcgt tttgcaacca gcttgttgcg cctgctgcaa
    30361 taacttcgtc atatgcgggt tatacacgca ttcagtgacc agcagtcccg gatgtaacag
    30421 actgatatca ttaaccaatg atttattctc aaggggtttc atacccactt ttgtgccatt
    30481 ggttaaaatg tcggcggaag ccagggcttc agcaaaggct tgctgatcgg cgagatcggt
    30541 aaccgtgacg acacaatcgg tgttttcgtt aacccgctgc gcgaaggcga gggctttatc
    30601 gaagaactta tcccgacggt taaagagttt aatttctttt aaaccttcaa ttgccccctg
    30661 cgcgccaatc gccgttgagg cacctccggc ccctaacagc accattgttt tgcctttgat
    30721 atcaaaaccg ctctctttaa tggcgcgaat atggcccgtg ccgtcggtgt tatagccgcg
    30781 caggtagcca tcatcattaa cgatggtgtt gatggcaccc accagtttgg ctgctggcgt
    30841 taattcatca acatattcac acgccagttg tttgttcggc attgatacac cagttccgcg
    30901 catttttaga gcttttaatc cttcaattgc tccgggaaag ctatcgttat ccacttcgaa
    30961 ggccatatag gtaaatggca atcccgcttt ttctaaggct ttattctgca tttcaggcga
    31021 taaactgtgg cggataggat aagccatcaa cccaatcaat tcgtattttg cggtaacatc
    31081 cataattaac tcctttaacc tctatgctta attgtgttta accgggtgct aaatttgcgc
    31141 tcgccaaacc gcacatcatt ctcaggaata ataaaaacgc ggtaatagcg gataaaaaca
    31201 ataattgcgg taatcagggc cagcagcgcg aaagtaaaat cgagaacaat gatatattgc
    31261 aggccgatgt tcgacagata accggtaatc agcggaataa caaagttagc cagtccaccc
    31321 atcatcatat aaatactggt gactttggct ttgctttttg ggaaaaactc tgacatgacc
    31381 gaaacaccga gctgtaaaat gccgccagct gctgagaaac cgataacaaa ggcgccggca
    31441 ttacacacca gtggagaagg gtacaggtag ataatggctg ctgttattgt tgccagtgca
    31501 gagttaaata cattagccca gatgggccgg accatttttt tcagtagtgc ggcaaaaata
    31561 aagacacaga ccagcgagcc catactgtaa taagagatgg tttttaatgc ctcagcttct
    31621 gacataccag caaaagccat cgcatatttg ggcatccaca ccacaatcac ataaaaggtc
    31681 gagaatgcgg ctacaccgaa cagtaccgat gaaacacctt ccagccagac taacggtttg
    31741 ctgttcattt gcggtaattc attagctacg ctggcgtcca ccaactggct ggggaatttg
    31801 cttttcaaca gcatcagcgt gatcagtaca aacagaatac ccggaataat cagcccatag
    31861 ccgtaccaga tattattgag caacatatag ctcaccagca ttgggtagaa catttgccca
    31921 aatgacacca tcgctttaac cagtatgacc gccgaaccag aggctttcgg aaagcattcc
    31981 atgagcgcgg ggtagccacc cgtatccagc gctgagttag cgatacctac gcacactgcc
    32041 agaccgtagg cgagagttaa attcgggcaa gcgggaatac caaagaagaa tagcagatac
    32101 attattactg ccattaatat caccgcccga cgaccaaact tatcggagat cacaccgaag
    32161 aataaaatac tgatcaatcg ccccaaaccg ataccggaaa ttaagtaggc aatgcccgcg
    32221 ttgtcagtgg aaaacttttc cgccagagat gacatatttt gggcaagcgt aataacacta
    32281 atgccgtgca ggaagtagct gaagtaaata caaagaacag ccaggataaa tggcgtgctg
    32341 aaagccttat tttgagacat tttcacagct ccttgctgaa agataatttc gttaccgtac
    32401 gtattgttct ccgacggttt acataattac tatactgaag atgattttcc ctgggtacat
    32461 ggcataaagt ttatgtttcc aggaatattg catgtcatag tttgtttttg atcgcgattt
    32521 tccttagtta gcgcagaaag agttatttat ccgtaagatc ttgcatgacc tgaaaagaat
    32581 attgcattac ccacccggag cgacattttc ctttagcgag tgatgccgcg tttttttgcg
    32641 gctgcgtaag ccgataaaca gtgccagtaa aaaaccgatg gcagcgatgg cggtatcgaa
    32701 ccacataata tcggcaatac ttctttggga cagatgagcc gtaatcaacg gaatagtaaa
    32761 ggtcgcaata ctgcccgcac tgtaatagat ccctgtagct ttacctttag cgtaagggaa
    32821 acgttcagcc attaacgtca ggccaatttg cacaacacct ccagcagaag taaaaccaat
    32881 gacaaaagaa aatattatca ccacataaaa tgtgggatgc aggcagacgg taaacagagc
    32941 aataaatgag ataaaggtgt acagcatcag taatgttgtt gggcgaacgg tattacgaat
    33001 gagtggagcg gtaataaata cacaaagcag cgaacccacg gtgtagatac tgagtagttt
    33061 gatcgacata gtgtatgaca tgcctgcaac aaattgtccg tactgtgcca gccactggct
    33121 aaccagataa aacgttgcca ttgagatata gccatataag gtataactgg ctaaatcgat
    33181 aattgaacag cgatgttccg tagagctggt ggttttcttt atgacaggta agcgacgacc
    33241 cggatggggt gggaacgtac aacggtataa aaacagagcg ttaataaaca taatgcctgc
    33301 agcaatcata aaggaccaac cgaaccacag ttcagcccac actaacaggc taatgattag
    33361 cggtaataaa aattgtccgc tggaaacaaa tgctttaatt aaaatattgg ctgtcccagg
    33421 tgagcgtgga aaagcttcca tcaaactggg ataagtgcct gcatcgagaa aactgtttgc
    33481 cattcccgcc agaaagccaa aaacataagc gataatgatg ttattggtct gcaggatgcc
    33541 aaaaaagaag gccatatagc agcacatccc gagcatgata aaagggcggc gaccaaagcg
    33601 atcggataat aatcctgcaa aaagcaagac acttaatcga ccaatgccca gcgatgagat
    33661 aactatcgag acacccgcgg cattagactg ccaaagtgtc tccagcgagg ccatattcag
    33721 gctcatcaaa atgacgccca taccatgcac caggtaatta aaatacaacc caagtgcggt
    33781 agggtaatag ggatttttca taggttctac ttagcaaagg tgctttaaaa agtgtagcga
    33841 tgttgtgtca gtatccgttc attattgttg taacaacgac aattcctttt tgtggagaaa
    33901 ttaacaaagc cgctccagat cgtgtgcata taattcagcg gctaccgatt gatagatctt
    33961 ccagtcaata aaattgccat cagggtagac ttgttgaaac gcggttaagt cggggaaaaa
    34021 acgcatggtg ttgttgccga tgcggttatt tattttctct atgatcgcat gaagatgttt
    34081 ttttctttgc tgacgcatag ccaacagttg ctcgactaca caatcaggaa tggcacacct
    34141 gccattttcc cattgacgcc agctttcact gtcaccggtc tgggcaatcc aggtcgcgca
    34201 ttcatcaata gtcatggcaa aaatatggcg gagtgcctgg agttcataag cgttcatcgt
    34261 gtaatggctg catgaaataa acaataaaat ataaataaca ttatcagcct gctgacggct
    34321 tgaagagagt cacagtatct tgtgcaacat tattggaaag agtgaggggc gaggtagcga
    34381 agcggaaaaa tgttaaaaaa aagcccatcg tgggagatgg gcaaagacta cacacagcaa
    34441 ttcgttgttt cactcagggg atttccatgc ttataaatca atatgttgat ttataaccgt
    34501 gagctaatag taggcatacg gactttttgc gtcgatcaga ttcgtctcat tagtttaaaa
    34561 actgtaaaga atttgcgaca ggtactacca gctgaatggc ggtaagcagg aaaatatggg
    34621 gaagagcaag caaatatcaa ttagcggtgg ttaacaatca ggcggcagag ccgcctgatg
    34681 agaaattact tattcggttt ctggatttcg ccaagttctt cgagaatttc ttccggttga
    34741 tccgtcggcg gtgggacctc ttccacccac gcggcaaaca gacgccagga aacggctaac
    34801 agaaccggac caataaacag accgatcatc ccgaaagcaa tcaaaccacc aataacgcca
    34861 gagaggatca ggatcagcgg taaatcggca cccatgcgaa ttaacattgg gcggatgacg
    34921 ttatccagcg tgccaaccac accgctccac actaacaata ccgttcccca ggtggtatcg
    34981 ccagtccagt agagccagat aatcgccgga atcagtaccg gcaacgggcc aagctggaca
    35041 aggcaggaaa ggatcattaa caccgttagc aaagttgcat aaggtacgcc ggatacggcg
    35101 aggccgatac cgccaagcac tgcctgtact aacgccgtca ccaccacacc cagcgccacc
    35161 gcgcggatag cctgtgccgc cagcagtacg gcggcatctc cgcgaacgcc tgccagacgg
    35221 gtcgcaaaat ggcgaatgcc ttgtgccacc tgttcaccgc gccagtacag cagggcactg
    35281 aagagaagca tcagcgcaca atgcaccata aagcgcccga tatgcgccgc ttgcccaacg
    35341 aaccaggtag tggtggtgcc aatataaggg cggactttcg ccatgatcgc cgtccccccc
    35401 atatccagca agttgtgcca gcctgcatac agcttcgcgc caatcaccgg aatggtatta
    35461 agccacgcca gatcgggtaa cgtcatgtca ccgctggaaa tggctttaat tagcgggccg
    35521 ctgccgtcga cgatactgtt aaccagcaaa gcaataggga tgataaacac catcactaat
    35581 aacagcgtca tcaccagaac ggcgagagag cggcggccaa acatgatctt ttgcaaacgt
    35641 aacaataccg gccaggtggc gataaccacc gtaccggccc atgcaaagcc gagaataaag
    35701 ggttgaacaa tccacagaca tgccacaatc atgatggcta aaaacagcac cgaaagcaga
    35761 atttgtgcga catccctggg ctgacgaaca tttaccataa ctacttttca ccttttttgt
    35821 gcgtcaaatc gtcgacgcga cgtgaacacg cgaaactcac ctgcataatg atgagcaatt
    35881 tcagcggttt ttaacaggcc gattctgccc ttaattctga tgggcgtgtt agaaaaaaat
    35941 gtggtacaac aactcaggca acacgcaaac gatttactcg tcgtatttca agttgcatat
    36001 gctgcggcta cgttcgttca ccccagttac ttactgttgt aagctcctgg agttcactcg
    36061 cttgccgcct tcctgcaact cgaaatccat agagtaaata aacatcacct ttctgtgcgc
    36121 tattgcagcc gcctgaaaga acagtgtatc gacctacatc acagaccgca ggaaagggtc
    36181 aatataatga ttccacagat ttcccaggca cccggcgtcg ttcaactggt gcttaatttt
    36241 ttgcaagagc tggagcaaca aggttttacc ggcgatacgg cgacaagtta tgctgatcgt
    36301 ctgacaatgt cgaccgacaa cagtatttac caacttctcc ccgatgcagt ggtatttccg
    36361 cgttcaaccg cagatgtggc gctgatcgcc cgtcttgccg cgcaggaacg ctattcatcg
    36421 ctgatcttta ccccccgcgg cggcggcacc ggcactaacg gtcaggcgct caaccagggt
    36481 attattgttg atatgtcccg ccatatgaac cgcatcatcg aaattaaccc tgaagagggc
    36541 tgggtgcgcg ttgaggccgg ggtgataaaa gaccaactca atcagtacct gaaaccgttc
    36601 ggctactttt ttgcaccaga actttcgacc agcaaccggg caacgctcgg cgggatgatc
    36661 aataccgatg catccggtca gggatcgctg gtctatggca aaacgtcaga tcacgtactt
    36721 ggcctacgtg cggtattgtt ggggggcgat attctcgata cgcaaccttt acccgtcgag
    36781 ctggcggaaa cgctgggtaa atccaatacc acaatcgggc gaatttataa cacggtttat
    36841 caacgttgcc gtcagcaacg ccagttaatc atcgacaact tccccaaact taaccgcttt
    36901 cttaccggtt acgatctgcg tcatgtcttt aacgatgaga tgaccgagtt cgatctgacg
    36961 cgcattctga cgggttcaga agggacgctg gcctttatta ccgaagcgcg gctggatatt
    37021 acgcgcttgc ctaaagtgcg ccgtctggtg aatgtcaaat atgactcttt tgactctgcg
    37081 ctgcgtaacg cgccgtttat ggttgaggcg cgggcgcttt cggtagagac ggtggactca
    37141 aaagtgctga atctggcgcg ggaagatatt gtctggcatt ccgtcagcga gttgattacc
    37201 gatgtgcctg acaaagagat gctcgggctg aacattgtgg aatttgctgg tgatgatgag
    37261 gcgctgattg acgagcgggt aaatgcactc tgtgcgcggt tggatgagct gatcgccagt
    37321 catcaagcag gtgtcatcgg ctggcagatg tgccgtgagc tggcgggggt tgaacgtatc
    37381 tatgcaatgc gcaaaaaagc cgttggtctg cttggcaatg ccaaaggtgc cgctaagcca
    37441 attccgtttg ctgaggatac ctgcgtaccg ccggaacatc tggccgatta tattgctgaa
    37501 tttcgcgcgc tgctcgacag ccacggctta agctacggta tgttcggtca cgtcgacgca
    37561 ggtgtcttgc atgtccgtcc ggcactggat atgtgcgatc cgcaacaaga gattttgatg
    37621 aagcaaatct ctgatgacgt ggtggcgctg actgcgaaat acggtggttt gttgtggggc
    37681 gagcacggca aaggttttcg cgctgaatac agcccggcgt ttttcggtga ggaacttttt
    37741 gcagaactgc gcaaagtgaa agcggcattt gacccgcata accgactcaa cccagggaag
    37801 atttgcccgc cagaaggtct cgatgcgccg atgatgaaag tggacgcggt gaagcgcggt
    37861 acattcgatc ggcagatccc cattgcggta cgccagcagt ggcgcggtgc gatggagtgt
    37921 aacggcaacg gtttatgctt caactttgat gcccgtagtc cgatgtgtcc gtcgatgaag
    37981 atcacccaga accggattca ttcaccgaaa gggcgcgcaa cgctggtgcg tgaatggctg
    38041 cgtttgttgg cggatcgcgg cgttgatcca ctcaaactgg aacaagaact gcctgaatcc
    38101 ggcgtcagct tgcggacgtt aattgcccgc acgcgcaata gctggcatgc gaataaaggc
    38161 gaatatgact tctcacacga agtcaaagag gcgatgtcgg gctgtctggc ctgtaaagct
    38221 tgttcgaccc agtgccccat caaaattgat gtgccggagt ttcgctctcg ttttctgcag
    38281 ctctatcaca cccgttattt acgcccgctg cgcgaccacc tcgtcgctac ggtcgagagc
    38341 tacgcgccgc tgatggcacg cgcgccgaaa acctttaact tcttcattaa ccagccgctg
    38401 gtgcgcaaac tctcggaaaa acatatcggc atggttgatt tgccgctgct gtcggtcccc
    38461 tcgctacaac aacaaatggt ggggcatcgc tcggcaaaca tgacgctgga acagcttgaa
    38521 tctctcaatg cagagcagaa agcgcgcaca gtgttggtgg tgcaggaccc ctttaccagc
    38581 tattacgatg cgcaagtggt ggcggatttt gtccgtctgg tcgaaaaatt aggtttccag
    38641 cctgtgttac tgccattttc gccaaatggc aaagcccagc atattaaagg ttttcttaat
    38701 cgttttgcga agacggcgaa aaagacggcg gatttcctca accgtatggc gaagctgggt
    38761 atgccaatgg tgggcgtcga tccggcgctg gtactttgtt atcgcgatga atataaactg
    38821 gcgctgggcg aggagcgtgg cgagtttaac gtcttactgg cgaatgaatg gctggcaagc
    38881 gcacttgagt cacagccagt ggctacagtc agcggtgaat catggtattt ctttggtcac
    38941 tgtaccgaag ttaccgcctt gccgggtgcg ccagcacaat gggccgcgat atttgcccgt
    39001 tttggcgcga aactggaaaa tgtcagcgtg ggttgctgcg gcatggcagg gacttacgga
    39061 catgaagcga aaaaccataa aaattcgctc gggatctatg agttatcctg gcatcaggct
    39121 atgcagcgac tgccgcgtaa ccgctgtctg gcgaccggat attcctgccg tagtcaggtc
    39181 aaacgggttg aaggcacggg ggtacgccat cctgtgcagg ctttactgga gattattaaa
    39241 tgatatggaa acgaaaaatc cccctggaag cactgaatgc catgggtgaa ggaaacatgg
    39301 tggggttact ggatattcgc tttgaacata ttggtgatga cacccttgaa gcgacaatgc
    39361 cagtagactc gcggacaaag cagcctttcg ggttgctaca tggaggtgca tccgtggtac
    39421 tggccgaaag tatcggttcc gttgccggtt atttatgtac cgaaggtgag caaaaagtgg
    39481 ttggtctgga aatcaatgct aaccacgtcc gctcggcacg agaagggcgg gtgcgcggcg
    39541 tatgcaaacc gttgcatctc ggttcgcgtc accaggtctg gcagattgaa atctccgatg
    39601 agaaagggcg tttgtgctgt tcgtcacgat tgacgaccgc cattttgtga cgttgtcacc
    39661 agaaaagtgt gacgcatatt gcttttggtc attatctgca cagtaaacgg tggtatactg
    39721 tgcaggtctt gtctaaaagc gaggatgcta tgtctactca acttgatccc acccagctgg
    39781 ccattgaatt tttacgccgt gaccagagca atctttcacc tgcccaatat cttaaacgtc
    39841 tgaaacaact agaacttgaa tttgctgacc tgcttactct ctcttctgct gaattaaaag
    39901 aagaaatcta ctttgcctgg cgtctgggcg tgcattgatc ctgacttaat gccgcaagtt
    39961 cgccaaatcg acctggatta acctccgtcc aggttcgatg accttcttaa tactgcttat
    40021 ctacccgcct gatattgttc aaaaataata ctaatcgcag tttgtgttaa aacggcggct
    40081 tagctttatg agattattct tatcgcccct tcaagagcta agccactgag agtgccggag
    40141 ataagcgccg gatggggtag aaacccttaa gcctgtgtcg cacagactta agggttttct
    40201 tatttctgaa cgctgcactt ttttacctat ctcttaaata gtcattttca atacaacttt
    40261 ttctttttca tcacagcctt aatgctcctt agataataat cattatctaa caatgagata
    40321 cctaattctt agcgtgcctg ttaacccaca catcagggtc tatgcttatt aaaagcaggc
    40381 aaaaagcatg ttttacggta aagcccctgc ctttgctggg ttgaactgat aatcattatc
    40441 actaacatgc tgttatacgc tgaaagcgat gaagtgaggt aaatcgatgg acatgcattc
    40501 aggaaccttt aatccacagg atttcgcctg gcaaggctta acgctgacac ccgcagccgc
    40561 tgtccacatc cgcgagctgg tggcaaagca gccgggtatg gtcggcgtgc gcttaggtgt
    40621 gaagcaaacg ggctgcgcgg gctttggcta tgtgctcgac agtgttagcg agccggacaa
    40681 agacgatctg ctgtttgaac acgacggcgc gaagctgttt gtcccgctgc aagcgatgcc
    40741 gtttattgat ggcacggaag tcgatttcgt tcgtgaagga cttaatcaga tattcaaatt
    40801 tcacaaccct aaagcccaga atgaatgtgg ctgtggcgaa agctttgggg tataggcggt
    40861 actatgtctc gtaatactga agcaactgac gatgtcaaaa cctggaccgg cggcccgctg
    40921 aattataaag aaggattctt cacccagtta gccaccgatg agctggcaaa ggggataaac
    40981 gaagaggtgg tgcgcgcaat ttcggcgaag cgtaatgagc cggagtggat gctggagttt
    41041 cgtctaaacg cctatcgcgc atggctggag atggaagaac cgcactggtt gaaagcgcac
    41101 tacgacaagc tgaattatca ggattacagc tactactcgg caccatcgtg cggtaattgt
    41161 gacgacactt gcgcgtctga acctggcgcg gtgcagcaaa ctggcgcgaa cgccttttta
    41221 agtaaagagg tggaggcggc gtttgagcag ttgggtgttc ccgtgcggga aggcaaagag
    41281 gtggcggtgg atgccatttt cgactcagtt tcggttgcca ctacttatcg cgaaaaactg
    41341 gcggagcagg gaattatttt ctgttccttt ggtgaggcga tccacgatca cccggaactg
    41401 gtgcgtaaat atctcggcac cgtggtgccg gggaatgaca acttctttgc cgcgcttaat
    41461 gcggcggtag cctctgacgg tacgtttatt tatgtgccta aaggcgtgcg ctgcccgatg
    41521 gaactttcca cctattttcg cattaacgcg gaaaaaaccg gacagtttga gcgcaccatt
    41581 ctggtggccg acgaagacag ctacgtcagc tacattgaag gctgttccgc tccggtgcgt
    41641 gacagctatc agttgcacgc ggcagtggtg gaagtcatca tccataaaaa cgccgaggtg
    41701 aaatattcca cggtacaaaa ctggttccct ggcgataaca acaccggcgg tattctcaac
    41761 ttcgtcacca agcgtgcttt gtgcgaaggc gaaaacagca aaatgtcatg gacgcaatca
    41821 gaaaccgggt cagcgattac gtggaaatat cccagctgca ttttgcgcgg cgataactcc
    41881 attggtgagt tttactcagt ggcgctgacc agcggtcatc agcaagcgga taccggcacc
    41941 aagatgatcc acatcggtaa aaacaccaaa tcgaccatta tctcgaaagg gatctctgcc
    42001 ggacatagcc agaacagtta tcgcggctta gtgaaaatca tgccgacggc aaccaatgcg
    42061 cgcaatttca ctcagtgcga ctcaatgctg attggcgcta attgtggggc gcataccttc
    42121 ccgtatgtcg aatgtcgcaa taacagcgca caactggagc acgaggcaac gacatcacgt
    42181 attggtgaag atcaactgtt ttactgcctg caacgcggga tcagcgaaga agacgccatc
    42241 tcgatgattg ttaacggttt ctgcaaagac gtgttctcag aactgccgct ggaatttgcc
    42301 gttgaagcac aaaaactcct cgccatcagt cttgaacaca gcgtcggata aggaataaac
    42361 atgttaagta ttaaagattt acacgtcagc gtggaagata aagctatcct gcgtggatta
    42421 agcctcgacg ttcgtcccgg cgaagttcac gccattatgg ggccaaacgg ttcgggcaaa
    42481 agtaccttat cggcaacgct tgccgggcga gaagattatg aagtgacggg cggcacggtt
    42541 gagttcaaag gcaaagattt gcttgcactg tcgccggaag atcgtgcagg cgaaggcatc
    42601 tttatggcct tccagtatcc ggtggagatt ccaggtgtca gtaaccagtt tttcctgcaa
    42661 acggcactta atgcggtgcg cagctatcgc ggccaggaaa cgctcgaccg ctttgatttt
    42721 caggatttga tggaagagaa aatcgctctc ctgaagatgc cggaagattt attaacccgt
    42781 tcggtaaacg ttggtttttc cggcggcgag aaaaagcgca acgatatttt gcaaatggcg
    42841 gtgctggaac cggagttatg cattcttgat gaatcggact ccgggctgga tatcgacgca
    42901 ttaaaagtgg tcgccgatgg cgtgaactcg ctgcgtgatg gcaagcgctc attcatcatt
    42961 gttacgcact accaacgcat tctcgactac atcaagcctg attacgttca tgtgctatat
    43021 cagggacgaa ttgtgaaatc cggcgatttc acgttggtca aacaactgga ggagcagggt
    43081 tatggctggc ttaccgaaca gcagtaacgc gctgcaacag tggcatcact tgtttgaagc
    43141 tgaagggaca aaacgctccc cgcaagcaca gcagcattta caacaattgc tgcgtaccgg
    43201 actgccgaca cgtaaacatg aaaactggaa atatacgccg ctggaagggc tgaccaatag
    43261 tcagtttgtc agcattgcgg gagagatatc cccacagcag cgagatgcct tagcgttaac
    43321 gttagacgct gtgcggctgg tatttgtcga tggacgttac gtgcctgcgc tgagcgatgc
    43381 gactgaaggc agcgggtatg aagtgagcat taacgacgac cgtcaggggt tacccgatgc
    43441 tattcaggcg gaagtgtttc tgcatttgac ggaaagcctg gcacaaagcg tgacgcatat
    43501 cgccgtgaag cgcggtcaac ggccggcaaa gccattgctg ttaatgcata tcacccaggg
    43561 cgtggcaggt gaagaggtga acactgccca ttaccgacat catctggagc tggcggaagg
    43621 tgccgaagca acggtgatcg aacattttgt cagccttaat gatgctcgtc attttaccgg
    43681 ggcacggttc tctatcaacg tcgcagcgaa tgcccacttg cagcatatca agctggcgtt
    43741 tgaaaacccg ctcagtcacc actttgccca taacgatttg ttgctggctg acgatgccac
    43801 cgcatttagc cacagtttcc tgctgggtgg cgcagtgtta cgacacaaca ccagtacgca
    43861 actcaatggc gaaaactgca cgctgcggat caatagcctg gcgatgccgg tgaaaaacga
    43921 ggtgtgtgat acccgtacct ggctggaaca caataaaggt ttttgtaaca gccgacagtt
    43981 gcacaaaact atcgtcagcg acaaaggccg cgcggtattt aacggtttga tcaacgtcgc
    44041 gcagcacgcc atcaaaacgg atggtcagat gaccaacaat aatctgctga tgggcaaact
    44101 ggcggaagtg gatacgaaac cgcagctgga aatctatgca gatgatgtga aatgcagcca
    44161 cggcgcgacg gtggggcgta ttgatgatga acagatgttc tatctgcgct cgcgcgggat
    44221 caatcagcag gatgcccagc agatgatcat ttacgccttt gctgccgaac tgacggaagc
    44281 actgcgtgat gaggggctta aacagcaggt gctgacccga atcggtcaac ggctgccagg
    44341 aggtgcaaga tgactttttc cgtcgacaaa gtgcgggccg actttccggt gctttcgcgt
    44401 gaggtaaacg gtttgccgct ggcttatctc gacagcgccg ccagtgcgca gaaaccgagc
    44461 caggtgattg acgccgaggc cgagttttat cgtcatggct acgcggcggt gcatcgcggt
    44521 attcatacct taagcgccca ggcgaccgag aaaatggaga acgtgcgcaa gcgggcatcg
    44581 ctgtttatta atgcccgttc ggcggaagag ctggtgttcg tccgcggcac gacggaaggg
    44641 atcaatctgg tcgccaatag ctggggcaac agcaacgtgc gggcgggcga taacatcatc
    44701 ataagtcaga tggagcacca cgctaacatt gttccctggc agatgctttg cgcacgcgtt
    44761 ggcgcagagc tgcgtgtgat cccgctcaat cccgacggta cgttgcaact ggagacgctg
    44821 cctacgctgt ttgatgagaa aactcgcctg ctggcaatta ctcatgtctc caacgtgctt
    44881 ggcacagaaa atccactggc ggaaatgatc acgcttgcgc accagcatgg cgcaaaagtg
    44941 ctggtggatg gcgctcaggc ggtgatgcat catccggtgg atgttcaggc gctggattgc
    45001 gatttttacg tgttttccgg gcataaactg tatggcccca ccgggattgg cattctttat
    45061 gtcaaagaag ccttgctaca ggagatgccg ccgtgggaag ggggcggttc tatgatcgcc
    45121 accgtcagcc tgagtgaagg cactacctgg accaaagcac cgtggcggtt tgaagccggt
    45181 acacccaata ccgggggcat cattggtctt ggcgcggcgc tggagtatgt ttcggcgcag
    45241 gggcttaata acatagccga gtatgaacag aatctgatgc attacgcgct atcacagctg
    45301 gaatctgtac cggatctcac tctctatggc ccacaaaaca ggcttggcgt tattgctttt
    45361 aatctcggta aacaccacgc ctatgatgtt ggcagttttc tcgataatta cggcattgct
    45421 gtgcgtaccg gacatcactg cgctatgcca ttaatggcct attataacgt ccctgctatg
    45481 tgtcgggcgt cgctggccat gtataacacc catgaagaag tggatcgtct ggtgaccgga
    45541 ctgcaacgta ttcaccgtct gctgggataa cagggaggca ctatggcttt attgccggat
    45601 aaagaaaagt tgctgcgtaa ttttttacgc tgcgccaact gggaagagaa atatctctac
    45661 attattgagc tgggccagcg tctgccagaa ttacgcgacg aagacagaag cccacaaaat
    45721 agcattcagg gctgccagag tcaggtgtgg attgtcatgc gtcagaatgc gcagggaatt
    45781 attgaattgc agggcgacag cgatgcggcg attgtgaaag ggcttattgc ggtcgtcttt
    45841 attctctacg atcagatgac gccgcaggat gttgtcaatt tcgatgtgcg tccgtggttt
    45901 gaaaaaatgg cactcaccca acatctcacc ccatctcgtt cacaaggtct ggaagcgatg
    45961 attcgcgcaa ttcgcgccaa agccgctgca cttagctaaa ctaaagggac agctttcatc
    46021 cggttggctc acattgccgg atggtcaatg tttacactga cctgcacagg agtgtttcgg
    46081 tttcaggtga acataagcgc catccgacgt ttcagcgtga gtctccggca aatacaggag
    46141 gtttacaatg aaacgcgcgt ctttgcttac actcacgctt atcggcgctt ttagtgccat
    46201 ccaggctgcc tgggcggttg attatccgct accaccaacc ggaagccgac tggttgggca
    46261 aaatcaaacg tatacggtgc aggaagggga taaaaacctt caggccatcg cccgacgttt
    46321 tgatactgcg gcaatgttga tccttgaagc caataacact atcgccccgg tgccaaaacc
    46381 cggtacgacg ataactattc cttcgcaact gttattacct gatgcgccgc gtcaggggat
    46441 tatcgttaac cttgcagagc tgcgccttta ttattatccg ccgggagaaa atatcgtgca
    46501 ggtttatccg ataggtattg gcttgcaggg gctggaaacg ccagtgatgg aaacgcgtgt
    46561 ggggcagaaa atccctaacc caacctggac gcctacggca ggcattcgtc aacgttcgct
    46621 ggagcgtggc attaaattac cgccagtcgt tcctgccgga ccaaataacc cgctaggacg
    46681 ttacgcactg cgcctcgcgc atggtaatgg cgaatacctc attcatggca ccagtgcgcc
    46741 ggacagcgtc ggtttgcgcg tcagttcagg gtgtattcgc atgaatgcac cggatattaa
    46801 agccttgttc tccagcgtgc ggacgggaac gccggtgaaa gtgatcaacg aaccagtgaa
    46861 atattccgtg gagcctaacg ggatgcgtta tgttgaagta catcgaccac tatcggcaga
    46921 agaacagcag aacgttcaga caatgccata cacactgcca gcaggcttta cgcaatttaa
    46981 agacaataag gctgtagatc agaagttagt cgataaagcg ttgtatcgtc gggcagggta
    47041 tccggttgcg gtgagcagtg gagcaactcc cacagccagc aatgcgcctt cagtagagtc
    47101 agcgcagaat ggtgaaccag agcaagggaa tatgttacgc gcgacgcagt agtggtaaat
    47161 agcagacaaa aaaatggcgc acaatgtgcg ccatttttca cttcacaggt actattactt
    47221 gcggtattta gtagccatgt tgtccagacg ctggttagca cgagctgcgt catctttagc
    47281 agcctgaacg tcggaacgca ttgcgttcac gtcgttgctc agctggtcaa ctttagcgtt
    47341 cagagtctga acgtcagaag acagctgatc gattttagcg ttgctggagc aacctgccag
    47401 cagagtagaa cccaggatta ccgcgcccag taccagttta gtagctttca ttattaatac
    47461 cctctagatt gagttaatct ccatgtagcg ttacaagtat tacacaaagt tttttatgtt
    47521 gagaatattt ttttgatggg aaggcactta tttttgatcg ttcgctcaaa gaagcatcga
    47581 acgctgggtt ttagataaaa aaattgagag aaaacaggat gcttccatcg gattcatctt
    47641 agataaagtg agattatata gtgaaatccg atttgatttt ttattgcttc tggttatcga
    47701 ttaaataaaa aaagcgccca tcagggcgct tcgatataca aattaattca caaaagcaat
    47761 attacaggac gtgaacagat gcggtgttag tagtgccgct cggtaccagt gcaccagaaa
    47821 ccataactac aacgtcacct ttgtgtgcca gaccgctctg cagagccagt tctttaccca
    47881 gacggtagaa atcatcagta gaagtgatct ctttaaccag ctgcggcaca acgcctttgc
    47941 tcagtaccaa ctgatgagcc gttttttcgt tggtggtcag tgccaggatg gtggcatccg
    48001 ggaagtattt acgtactgcg cgagcagatt taccgccctg ggtagcaacc acgatcagcg
    48061 gagcatccag tttttcagca gtttcaacgg caccacggca taccgcttcg gtaatgcgca
    48121 gtttacggtt gtcattgttg aactcgagac ggctgttcat cacgcggtcg gtacgttcgc
    48181 agatggtcgc catgatagaa accgcttcca gcgggtattt accttttgcg gattcaccag
    48241 acagcatcac tgcgtcagta ccgtcgagga tggcgtttgc aacgtcaccg gcttctgcgc
    48301 gagtcgggcg tgggtttttg atcatggaat ccagcatctg ggtcgcagtg ataacgactt
    48361 tacgtgcacg gatacatttt tcgatcatca tcttctgggc gaagataact tcttctaccg
    48421 ggatttctac acccaggtcg ccacgcgcaa ccatgatgcc gtcagaggct tcgaggattt
    48481 cgtcgaagtt gttgaggcct tcctggtttt cgattttgga gatgatgtgg atgttttcgc
    48541 cgccgtgcgc tttcaggtgc tcacggattt cgataacgtc agaacgctta cgaataaagg
    48601 aagcagcgac aaagtctacg ccttgttcgc aaccaaagat caggtcctgt ttgtcttttt
    48661 cagccagtgc tggcagggca atggaaacgc caggcaggtt cacacctttg ttttcaccca
    48721 ggtcgccgtt gttcagcact ttacagataa ctttgttacc ttcaatggca gtaacttcca
    48781 taccgatcag accatcgtca accagtacgg tgttgccaac agacaggtca gttgtgaaac
    48841 cttcatacgt taccgcaacc atttcgctgt tgccgataac agatttatca gtagtgaaag
    48901 taaaggtctg accagctttc agagaaacgt cgttaccgcc ttccagtttc atggtgcgga
    48961 tttccggacc tttggtatca agcaggatag cggcggtttt accagttttg ctcatcacgt
    49021 tgcgcagatt ctgaatgcgc tgaccgtgtt ctgcatagtc accatgagag aagttcagac
    49081 gcataacgtt catgccagcg tccagcattt tagctaacat ctcttcagat tcggttttcg
    49141 gtccgatggt gcaaacaatt ttggtctttt tcatgacagt cttagtcttt aagttgagaa
    49201 ggatgggaga aacttgcttt ctgggcgcac tgccggaaag cgaatccggt attacgaaag
    49261 tggtggtgcc tcgctctaag gataggtgac atcgaaaaag cgtgcagagg aatgtgtgct
    49321 tgtgtctcag cccaacgtga gtgcctgttt ttgagtcgtt gagttctaca gtccaaagtg
    49381 ctgaaaccat tcaagagtca attggcgcgc attatacgct gaaattaatc caaaaggaaa
    49441 acaaaaacag cgtttcaaaa aatatacaac ttgttagaca ataaattgtt atcaaagatt
    49501 taacgttcca gggaaaaggt tacgcaaaaa cttcgctgtt tgcgcaacgt ctggtgccgg
    49561 ggttgtcact gtgaagataa gtgaaagttc atccggtgta taaatacatc atcgctcaat
    49621 tgctacagtt aaaataattt atcgcgttat ttgtctgatt ttgtttgatt tttgtttaat
    49681 tggttagatt gatatatgct gatagttggc atgataaata ggtataaaga atgggaaatc
    49741 ggacaaaaga ggatgaactg taccgagaaa tgtgcagagt tgtcggtaaa gttgtgctgg
    49801 aaatgcgcga tctggggcag gaaccaaagc atattgttat cgcaggcgta ttacgtacag
    49861 cattagccaa taaacgcatt cagcgcagtg agctggaaaa gcaggcgatg gaaaccgtca
    49921 ttaacgcact ggtaaaatga ccattcagga tgaggcgata agcttcatcc tgatattctt
    49981 gcttcagttg cggtcc
//
LOCUS       DAZKIP010000019        48816 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZKIP010000019 DAZKIP010000000
VERSION     DAZKIP010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.019"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            168..1385
                     /gene="alaA"
                     /locus_tag="ACHOUK_001372"
     CDS             168..1385
                     /gene="alaA"
                     /locus_tag="ACHOUK_001372"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFP9896848.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPCGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1469..2068
                     /gene="yfbR"
                     /locus_tag="ACHOUK_001373"
     CDS             1469..2068
                     /gene="yfbR"
                     /locus_tag="ACHOUK_001373"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFP9896849.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2127..3959)
                     /locus_tag="ACHOUK_001374"
     CDS             complement(2127..3959)
                     /locus_tag="ACHOUK_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFP9896850.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4046..4696)
                     /gene="hxpA"
                     /locus_tag="ACHOUK_001375"
     CDS             complement(4046..4696)
                     /gene="hxpA"
                     /locus_tag="ACHOUK_001375"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFP9896851.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4707..5201)
                     /locus_tag="ACHOUK_001376"
     CDS             complement(4707..5201)
                     /locus_tag="ACHOUK_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFP9896852.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5284..5739)
                     /gene="yfbV"
                     /locus_tag="ACHOUK_001377"
     CDS             complement(5284..5739)
                     /gene="yfbV"
                     /locus_tag="ACHOUK_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFP9896853.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6077..7279
                     /gene="ackA"
                     /locus_tag="ACHOUK_001378"
     CDS             6077..7279
                     /gene="ackA"
                     /locus_tag="ACHOUK_001378"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFP9896854.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7354..9498
                     /gene="pta"
                     /locus_tag="ACHOUK_001379"
     CDS             7354..9498
                     /gene="pta"
                     /locus_tag="ACHOUK_001379"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFP9896855.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIDSLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9688..11208
                     /gene="yfcC"
                     /locus_tag="ACHOUK_001380"
     CDS             9688..11208
                     /gene="yfcC"
                     /locus_tag="ACHOUK_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFP9896856.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRLLTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11241..11783)
                     /gene="yfcD"
                     /locus_tag="ACHOUK_001381"
     CDS             complement(11241..11783)
                     /gene="yfcD"
                     /locus_tag="ACHOUK_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFP9896857.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(11841..12392)
                     /gene="yfcE"
                     /locus_tag="ACHOUK_001382"
     CDS             complement(11841..12392)
                     /gene="yfcE"
                     /locus_tag="ACHOUK_001382"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFP9896858.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12448..13092)
                     /gene="yfcF"
                     /locus_tag="ACHOUK_001383"
     CDS             complement(12448..13092)
                     /gene="yfcF"
                     /locus_tag="ACHOUK_001383"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFP9896859.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLLFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPECLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13228..13875
                     /gene="yfcG"
                     /locus_tag="ACHOUK_001384"
     CDS             13228..13875
                     /gene="yfcG"
                     /locus_tag="ACHOUK_001384"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFP9896860.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFL
                     LISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            13932..14294
                     /gene="folX"
                     /locus_tag="ACHOUK_001385"
     CDS             13932..14294
                     /gene="folX"
                     /locus_tag="ACHOUK_001385"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFP9896861.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14315..15208
                     /locus_tag="ACHOUK_001386"
     CDS             14315..15208
                     /locus_tag="ACHOUK_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFP9896862.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVMTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGIDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNKFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPIRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15256..16146)
                     /gene="rpnB"
                     /locus_tag="ACHOUK_001387"
     CDS             complement(15256..16146)
                     /gene="rpnB"
                     /locus_tag="ACHOUK_001387"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFP9896863.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYLYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDVGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLEKGFD
                     HEVILTLTRLSPDDLIAQSH"
     gene            complement(16343..17116)
                     /gene="hisP"
                     /locus_tag="ACHOUK_001388"
     CDS             complement(16343..17116)
                     /gene="hisP"
                     /locus_tag="ACHOUK_001388"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFP9896864.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17124..17840)
                     /locus_tag="ACHOUK_001389"
     CDS             complement(17124..17840)
                     /locus_tag="ACHOUK_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9896865.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(17837..18523)
                     /gene="hisQ"
                     /locus_tag="ACHOUK_001390"
     CDS             complement(17837..18523)
                     /gene="hisQ"
                     /locus_tag="ACHOUK_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFP9896866.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAARAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18613..19395)
                     /gene="hisJ"
                     /locus_tag="ACHOUK_001391"
     CDS             complement(18613..19395)
                     /gene="hisJ"
                     /locus_tag="ACHOUK_001391"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFP9896867.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19616..20398)
                     /gene="argT"
                     /locus_tag="ACHOUK_001392"
     CDS             complement(19616..20398)
                     /gene="argT"
                     /locus_tag="ACHOUK_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFP9896868.1"
                     /translation="MKKSILALSLLVGISAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20664..21233)
                     /gene="ubiX"
                     /locus_tag="ACHOUK_001393"
     CDS             complement(20664..21233)
                     /gene="ubiX"
                     /locus_tag="ACHOUK_001393"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFP9896869.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21328..22845)
                     /gene="purF"
                     /locus_tag="ACHOUK_001394"
     CDS             complement(21328..22845)
                     /gene="purF"
                     /locus_tag="ACHOUK_001394"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFP9896870.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIEAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(22882..23370)
                     /gene="cvpA"
                     /locus_tag="ACHOUK_001395"
     CDS             complement(22882..23370)
                     /gene="cvpA"
                     /locus_tag="ACHOUK_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFP9896871.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGLLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23902..24564)
                     /gene="dedD"
                     /locus_tag="ACHOUK_001396"
     CDS             complement(23902..24564)
                     /gene="dedD"
                     /locus_tag="ACHOUK_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFP9896872.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVVPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24554..25822)
                     /gene="folC"
                     /locus_tag="ACHOUK_001397"
     CDS             complement(24554..25822)
                     /gene="folC"
                     /locus_tag="ACHOUK_001397"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFP9896873.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVCYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKTLPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVLDARRSGGK"
     gene            complement(25892..26806)
                     /gene="accD"
                     /locus_tag="ACHOUK_001398"
     CDS             complement(25892..26806)
                     /gene="accD"
                     /locus_tag="ACHOUK_001398"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFP9896874.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26962..27621)
                     /locus_tag="ACHOUK_001399"
     CDS             complement(26962..27621)
                     /locus_tag="ACHOUK_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFP9896875.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(27704..28516)
                     /gene="truA"
                     /locus_tag="ACHOUK_001400"
     CDS             complement(27704..28516)
                     /gene="truA"
                     /locus_tag="ACHOUK_001400"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFP9896876.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28516..29529)
                     /locus_tag="ACHOUK_001401"
     CDS             complement(28516..29529)
                     /locus_tag="ACHOUK_001401"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFP9896877.1"
                     /translation="MSEGWNIAVLGTTGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFVQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29595..30731)
                     /gene="pdxB"
                     /locus_tag="ACHOUK_001402"
     CDS             complement(29595..30731)
                     /gene="pdxB"
                     /locus_tag="ACHOUK_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFP9896878.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHERTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADCGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30830..31825
                     /gene="flk"
                     /locus_tag="ACHOUK_001403"
     CDS             30830..31825
                     /gene="flk"
                     /locus_tag="ACHOUK_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFP9896879.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHCPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMTLKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31822..33000)
                     /locus_tag="ACHOUK_001404"
     CDS             complement(31822..33000)
                     /locus_tag="ACHOUK_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9896880.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALALTTMALPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33275..34495)
                     /gene="fabB"
                     /locus_tag="ACHOUK_001405"
     CDS             complement(33275..34495)
                     /gene="fabB"
                     /locus_tag="ACHOUK_001405"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFP9896881.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34654..36660
                     /gene="mnmC"
                     /locus_tag="ACHOUK_001406"
     CDS             34654..36660
                     /gene="mnmC"
                     /locus_tag="ACHOUK_001406"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFP9896882.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEARFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     ISQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNGFTFAR
                     RLYDSLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPEELAVAVEANAVEQITGVT
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQDLSRKDDCWLLTFAGDQ
                     QATHSVVVLANGHQISRFSQTSSLPVYSVAGQVSHIPTTPELAKLKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEETAYSEDNQQQNRQRLIDCFPHAQWAKTVDVSKKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDKAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36781..37059)
                     /locus_tag="ACHOUK_001407"
     CDS             complement(36781..37059)
                     /locus_tag="ACHOUK_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFP9896883.1"
                     /translation="MIAEFESRILELIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(37093..37641)
                     /gene="epmC"
                     /locus_tag="ACHOUK_001408"
     CDS             complement(37093..37641)
                     /gene="epmC"
                     /locus_tag="ACHOUK_001408"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFP9896884.1"
                     /translation="MNSTHHYEQLIEIFNSCFADDFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARREQVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37641..38450)
                     /locus_tag="ACHOUK_001409"
     CDS             complement(37641..38450)
                     /locus_tag="ACHOUK_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFP9896885.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(38450..39274)
                     /gene="mepA"
                     /locus_tag="ACHOUK_001410"
     CDS             complement(38450..39274)
                     /gene="mepA"
                     /locus_tag="ACHOUK_001410"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFP9896886.1"
                     /translation="MNKTAIALLALLASSVSLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRRTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39278..40363)
                     /gene="aroC"
                     /locus_tag="ACHOUK_001411"
     CDS             complement(39278..40363)
                     /gene="aroC"
                     /locus_tag="ACHOUK_001411"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFP9896887.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40398..41330)
                     /gene="prmB"
                     /locus_tag="ACHOUK_001412"
     CDS             complement(40398..41330)
                     /gene="prmB"
                     /locus_tag="ACHOUK_001412"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFP9896888.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKAQLLAAREHFAIYKD"
     gene            41496..42047
                     /gene="smrB"
                     /locus_tag="ACHOUK_001413"
     CDS             41496..42047
                     /gene="smrB"
                     /locus_tag="ACHOUK_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311241.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFP9896889.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKVSEVPV
                     KRLIQEQVDASHYFSDEFQPLLNADGPVKYVRPGVDHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(42218..43060)
                     /locus_tag="ACHOUK_001414"
     CDS             complement(42218..43060)
                     /locus_tag="ACHOUK_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFP9896890.1"
                     /translation="MKKWTIFLTSLILLALSLETPKCYAGDKLMSASFSSTKIYYAMK
                     NVTASGSLYFYVTVVTPGEVSYGQYNSNARKGDTLKLISWSGSGPAPTLVLTDYRRTD
                     TSNCPGINTRVFVCAYMTFNVTVESDNYGCPWIASFYSVSEAFGFGTYTSPTVHDSIC
                     PTIPVASFDISWSDNYVSHNKALRLQSDGSTITTTLSTYLMESGKLCDGSIFDSRGAY
                     CRAVSDLLTFTSYGCDNAKVTVTPSRQPLTDRKLHDIVVQVNTSSREPIDSTCRFQYV
                     LNEL"
     gene            complement(43062..43583)
                     /locus_tag="ACHOUK_001415"
     CDS             complement(43062..43583)
                     /locus_tag="ACHOUK_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9896891.1"
                     /translation="MKNNRAWALISGLILFSGTAPAADNLHFTGNLLGKSCTPVINGN
                     LLAEIHFPTIAASDLMQRGQSDRVPLVFQLKDCKSTTAFNVKVTLMGTEDTDLPGFLS
                     IDSSSSATGVGIGIETAGGAAVPINSTTGASFPLNQGNNSVNFNAWLQTVNGRNVTSG
                     DFTATMTVTFEYF"
     gene            complement(43580..44050)
                     /locus_tag="ACHOUK_001416"
     CDS             complement(43580..44050)
                     /locus_tag="ACHOUK_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9896892.1"
                     /translation="MKRISLLMLWSFSFMALSNVSFHGYLVQPPNCSISNGQNIELTF
                     RDVNIDDINGSNYEQVVPYTITCDTAVRDPQMEMTLTWSGTQSDFDDSAVATDLNGLG
                     IHLKQAGSDFKLHTPLVVNETSLPVLTAVPVKKSGVDLPESDFEAWATLQVDYQ"
     gene            complement(44047..44547)
                     /locus_tag="ACHOUK_001417"
     CDS             complement(44047..44547)
                     /locus_tag="ACHOUK_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9896893.1"
                     /translation="MKGAYSAWAWLLLCSIVSTPVMAGSKSVAMVLRVLVDAPPPCTV
                     TGASVEFGNVFISKIDGVSYKRPIDYSLVCNNLAMDDLRLNMQATTTVINGETVIDTG
                     IAGFGIRIQKVSDHSILDLTPGAWLPFNFSSGALALEAVPVVQSGVSLTAAEFSASAT
                     IVVDYQ"
     gene            complement(44558..45316)
                     /locus_tag="ACHOUK_001418"
     CDS             complement(44558..45316)
                     /locus_tag="ACHOUK_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP9896894.1"
                     /translation="MTNLFCSARTAATTLALLITAASLSVQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKMPYLAQSWMEDGQGNRISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENVSMKNPWQHKV
                     TLTLSGQEFTVNNPTPYFVVISNASAQKNGNPAAGFSPMVISPKTSVPLKVKMDSVPV
                     LTYVNDYGARLPLFFRCTGNTCQVDEEQNSKGQG"
     gene            complement(45339..47978)
                     /locus_tag="ACHOUK_001419"
     CDS             complement(45339..47978)
                     /locus_tag="ACHOUK_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFP9896895.1"
                     /translation="MPDHSLFRLRVLPCCVALAMSGSYVNAWAENEIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLTEEYDIYWYASENDASKTYACLTPELV
                     AQFGLKEDVAKNLQWIHDGKCLKPGQLEGIDIKADLSQSALVISLPQAYLEYTDINWD
                     PPSRWDDGISGLIADYSITAQTRHEENGGDDSNEISGNGTVGVNLGAWRLRADWQTDY
                     LHSKSNDDDVINGDDTQKNWEWSRYYAWRALPSLKAKLGLGEDYLNSDIFDGFNYVGG
                     SISTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQLGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDINTASMPFLTRPGQVRYKLMMGRPQEWGHHVEGGFFSGG
                     EASWGIANGWSLYGGALADEHYQSAALGVGRDLSVFGAVAFDITHSHTRLDKETAYGK
                     GSLDGNSFRLSYSKDFDELNSRVTFAGYRFSEENFMTMSEYLDASDSEMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDRDEQTNYNVMLSHYFNLGSIRNMSISMTGYR
                     YEYDNQADKGVYISLSMPWGDSSTISYNGNYGSGSDSSQVGYFSRVDDATHYQLNVGT
                     SDNHSSVDGYYSHDGSLAQVDLSANYHEGQYTSAGISLQGGATLTAQGGALHRTQNMG
                     GTRLLIDADGVAGVPVEGNGAAVYTNMFGKAVVADVNNYYRNQAYIDLNNLPENAEAT
                     QSVVQATLTEGAIGYRKFSVISGQKAMAVLRLQDGSYPPFGAEVKNDSAQNVGLVDDD
                     GNVYLAGVKPGEHMIVSWGGVAHCDIHLPDPLPADLFNGLLLPCQQTGAISPSMPHEI
                     KPVIQEQTQQVMPTEAPVSVSAN"
     gene            complement(48060..48623)
                     /locus_tag="ACHOUK_001420"
     CDS             complement(48060..48623)
                     /locus_tag="ACHOUK_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9896896.1"
                     /translation="MSKFVKTAIAATMVMGAFASTSTIAAGNNGTARFYGTIEDSPCS
                     IVPDDHKLEVDMGDIGSGILKNNGTSTPKAFQIHLQDCVFDTQTTMTTTFTGNASSTN
                     SGNYYTIYNTDTGAAFNNVSLAIGDAQGTSYKSGAGIEQKIVNDTATNKGKAKQTLDF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        11985 a        13147 c        12184 g        11500 t
ORIGIN      
        1 cacctcatta ttttgtcatt attaagttat taacagcaca atcgagcctt cccctctggc
       61 aaaatcttat tctgcagacc ttcaaaacac cgtcctgggg gagtacattg ttctaagctg
      121 acttccacgg cagggagtgg cgataacagc aaaaaaggtc aagattcatg tcccccattg
      181 aaaaatccag caaattagag aatgtctgtt atgacatccg tggtccggtg ctgaaagaag
      241 caaaacgcct ggaagaagaa ggtaacaagg tactgaaact gaacatcggc aacccagccc
      301 cgttcggttt tgacgcgcca gatgaaatcc tcgttgacgt gatacgcaac ctgcctaccg
      361 ctcaagggta ttgcgattcc aaaggtcttt actccgcgcg taaagccatc atgcagcact
      421 atcaggctcg tggcatgcgt gatgttaccg tggaagatat ttacatcggc aatggtgtat
      481 cggagcttat cgttcaggca atgcaggcat tgctgaacag cggggacgaa atgttggttc
      541 ctgcaccaga ttacccacta tggaccgcgg cggtttcgct ttccagcggt aaagcggtgc
      601 attatctttg cgatgaatcc tctgactggt tcccggacct cgatgatatt cgcgctaaaa
      661 ttacgcctcg tacgcgtggg atcgttatta tcaacccaaa taacccaacc ggcgcggtat
      721 attccaaaga gcttttaatg gagattgtgg agattgcacg tcagcataat ctcattatct
      781 tcgccgatga aatttatgac aaaattctct acgacgacgc tgagcatcac tcaattgcgc
      841 cgctggcacc tgacctgctg accattacct ttaatggact gtcgaaaacg taccgcgttg
      901 caggcttccg tcaggggtgg atggtgttga acgggccgaa aaaacacgcc aaaggctaca
      961 tcgaaggtct ggaaatgctg gcttcaatgc gcctgtgtgc taacgttcct gcgcaacacg
     1021 ccattcagac cgcgctgggt ggttatcaga gcatcagtga atttattacc cctggcggtc
     1081 gtctttatga gcagcgtaac cgcgcgtggg aattgatcaa cgatattccg ggcgtttcct
     1141 gcgtgaaacc ttgtggtgcg ctgtacatgt tcccgaaaat cgacgccaaa cgctttaaca
     1201 ttcacgacga tcagaaaatg gtgctggatt tcctgttgca ggaaaaagtt ctgttggtgc
     1261 aagggacggc attcaactgg ccgtggccgg atcacttccg cattgtcacg ctaccgcgtg
     1321 tcgatgatat cgagctgtcg ttgagcaagt tcgcgcgttt cctttctggt tatcatcagc
     1381 tgtaatctta atttcactgc cggagattgc atccggcagt gttatcccgc cacaatgacc
     1441 tgatgatgtc atcatacgta aggtcactat gaaacagagc catttttttg cccatctctc
     1501 ccgcctgaaa ctcattaacc gctggccgct catgcgcaac gtgcggacgg aaaatgtgtc
     1561 cgaacacagt ttgcaggtag cgatggtcgc ccatgcgctg gcagctatca aaaatcggaa
     1621 atttggcggt aatgtcaacg ccgaacgtat cgctttactg gcgatgtacc acgatgcctc
     1681 agaagtgctc accggcgatc tccctactcc ggtgaaatac ttcaattcgc aaatcgctca
     1741 ggagtacaag gctattgaaa aaatcgctca gcaaaaactg gtcgatatgg ttccggaaga
     1801 gctgcgggat atctttgcgc cgttaattga cgagcatgca tatagcgatg aagaaaaatc
     1861 gctggtgaaa caggcagatg cactgtgtgc atatctgaaa tgtctggaag aactcgcggc
     1921 gggaaataat gaattcttgc tggcaaaaac gcgactggaa gcgacgcttg aagcgcgccg
     1981 cagccaggag atggactact tcatggaagt atttgttccc agcttccatc tttcgctcga
     2041 tgagattagc caggattcac cgctgtaagc agctggagtc tgcgtcgcat caggcaataa
     2101 gcgccggatg cgacatcagg ctcttgtcaa aacggaaaca gcatcgggat catcaccaca
     2161 caaaccgcca tcacgataat ggtgaacggt acccccagct tcacaaagtc actgaagctg
     2221 taatttcccg gacctaaaac caatgtgtta acaggtgaag aaaccggtgt cataaaggcg
     2281 gcggatgctg ccatcgccac gaccatcgcg aatggataag gcgacacccc catcgttttg
     2341 gcagcagcca gcgcaatcgg agccatcaac accgccgtcg cggtattaga gataaatagc
     2401 ccaataaccg ccgacaagac aaacaaacaa cccagcatca tatgtggccc gtaaccaccg
     2461 ccaatgtcca tcagcccttt caccgccagc gcgacacctc ccgttttctg taatgccaca
     2521 gcaaacggca tcatcccaac gatcaaaata atgctcggcc agtgaatgga tttataggcg
     2581 ctttcagcat ctatacagcg gaatttcccc atcagcaggc aggcgatgat agcggcaaca
     2641 ggattaggaa tttcatctgt cagcattaac gccaccatta gcaccagaca gaaaatggca
     2701 tggggtgcct ggctgtgcgc gggcgatgct tcactcacct cttccggtaa gttcagcgct
     2761 acgaagtcgc ggccctgttt ggccagcata ccgatcagtt tccagttacc cacaaccagg
     2821 atgatatcgc ccagcagcag aggctcatcc gccagcgaac cttccagcgc cacgccattg
     2881 cgctttagcc ccaccacatt cagtccgtag cgggtacgaa aaccaatttc gcgcaccgat
     2941 ttaccaatca gttctgactc aggaattaat gaaatctctg ccatgcccac atcaagggcc
     3001 tggtcagaaa aatactcgcc gcgcagtacc atcggctcca gcaattgctc actacaaaat
     3061 tgccggagat cgacatcagc cgcagacata tcaataagca aaacgtcacg cgcgcgaaat
     3121 tcagaaaccc cattaacgtt cacgataacg cgacgaaaac gccgccagcg ttcaacaccg
     3181 atgacgttag cgccataacg ctcacgtaat ttgagatcat ccagccgttg accaatcatt
     3241 ggcgatccgg ggcgaatagc cagacgtcgc gcacgcccgg tcagtcgata ttcacggata
     3301 agatcgcgaa aggttcgacg cgtccagcct tcgcgctgcg gggtctgggt atcccctttc
     3361 agcatgaaac gcatcactaa catatacaaa atacccagca ccagcacgac caggccaata
     3421 ggtgttacgc taaagaaact gaagccgtga tagccttcac gcagcaattc actgttgact
     3481 accaggttcg gcggcgtcgc caccagcgtc atcatgccgc taatcagccc ggcaaaactt
     3541 aacggcatca tcagacgtga cggcgaagtt tgcatacgca tggcaacgct taacaccacg
     3601 ggaataaaga tagcgacaac gccggttgaa ctcataaacg cgccaagccc cgcgacggtc
     3661 agcatcaaca aaaccaacat tttgatttca ctgttgcccg caactttgac cagccatgtt
     3721 cccattacgg tggcaacacc ggtacggacc aaaccatcgc caataataaa caaggcggca
     3781 atcaggacaa cgttaggatc agaaaagcca gaaaatactt ctgggactgt cagcgttccg
     3841 cttaatgcaa acgcgacaat aacaaacaaa gcgaccgcat ccatacgcac tctgcccgtc
     3901 gcaaacaaga cgatggcaac ggccagtaat gaaagaaccc aaatcaattc accgttcaca
     3961 acttatcctt gttaattgag ggggatgact tgattctgcc ataaaaaagc cccgacgaga
     4021 cggggctaaa tcatgatcag gtgtttcact gaataataac atcgccattt ggctgtttgg
     4081 tcacagtaat ttgctccaga ctgtggagga ccaaatcgac ctcattcagg cgcggggtat
     4141 ctgccggagc gttaaccgca atgacatggc aacccgccgc caggccagaa agcacgccag
     4201 cgggagcatc ttccaccacc acacactcct gcggcgcaag ccccagcagc tgcgcgccta
     4261 acagatacgc atcaggttct ggttttccgc gcttcactcg ctcagcggtt acaaacacct
     4321 ctggtgcggg aagccccgct attttatggc gcgctcgcgc taccggcatg gagccagaag
     4381 tcacaatggc ccacggaata cctgctttat tcaaatgatt gagtaaggcg attgcccccg
     4441 gaagcgcggt aataccttcg gtttccgtgg cctcgatttg ctccaaacgc gtaaactcgg
     4501 cggcaatatc agcctcggat ttgcccgcca taaaatggcg cagagaggtg atcgcctgtt
     4561 taccgtgaat gaaagccagc acctcttccg gcgctaaccc atgacgtctg gcccagttgc
     4621 tccacgcccg ttctaccgca ggcagggaat ccaccagcgt tccatcaaga tcaaacagaa
     4681 aacctttgca ccgcacgcgg gcctcctcag gcgttgataa tttgattaat ttcgttggcg
     4741 ctcaaatggt actgacgcgg gcaggcatgc cacacattaa gcatgcgctg atatttttcc
     4801 cacattggcg tctgggcgtt aaaaccgtga gttccggcgt caaaatgggt atagcgccct
     4861 tccacattaa ccataaagcg gacataaccg aggtaacgtg cttcagtggc ggcgtcaaag
     4921 ccgaggaagg tgacacgacg ttcatcgatg gattgctggt cctgcaaatt agaccaggaa
     4981 acatgcaacg catgatacat ctccataatg tcgatgatag tgcggcaggt ttcttctttc
     5041 agctcgccaa actcgcgatc cagttcacgc atctgtaatc cataaccccg ctcaatgatt
     5101 gtttgcaggc gacggtaacg ttcagcattt gccggatcga gcatagtcat catcttgtac
     5161 tggttagaca aaataagacg ttgcgcgttg gtcatttcca ttgttgactc ctgtatcact
     5221 ctactacggt gaaaaaaaag aaggctgagt atgccttctt ttatatgcgt aatcaggggt
     5281 caattacaaa tcatcaagga aagttttatc cagttgtttg aaggcacgct taagcgtgtc
     5341 agctaatgcc tggtaatcag gcttgccttc aacgggtgcc aacacctgtc cagactcctg
     5401 caatttaccg cgaacttcat aaaaccagtt gaggattgca gggggtaatg gcgtgacaga
     5461 acgcttgccc agccaccaca atccctgcat gggtaaactt aaggcgaaca gggcagtggc
     5521 aactgccggc ccaagctgac cgcccagggc aatctgccag cagagagtaa atacggcgat
     5581 cggcggcata aaacggatcg cataacgcgt catcttgata acgcgatttt cgacaaagac
     5641 cggggcaagg cgtttttcca gcggccacgt ctttgagtaa tgctgtcccc ggcgaaacaa
     5701 gctaaaaaaa ttaacagaac gattatccgg cgttgacatg cttcacctca acttcacata
     5761 taaagattca aaaatttgtg caaattcaca actcagcggg acaacgttca aaacattttg
     5821 tcttccatac ccactatcag gtatccttta gcagcctgaa ggcctaagta gtacatattc
     5881 attgagtcgt caaattcata tacattatgc cattggctga aaattacgca aaatggcata
     5941 gactcaagat atttcttcca tcatgcaaaa aaaatttgca gtgcatgatg ttaatcataa
     6001 atgtcggtgt catcatgcgc tacgctctat ggctccctga cgttttttta gccacgtatc
     6061 aattataggt acttccatgt cgagtaagtt agtactggtt ctgaactgcg gtagttcttc
     6121 actgaaattt gccatcatcg atgcagtaaa tggtgaagag tacctttctg gtttagccga
     6181 atgtttccac ctgcccgaag cacgtatcaa atggaaaatg gacggcaata aacaggaagc
     6241 ggctttaggt gcaggcgccg ctcacagcga agcgctcaac tttatcgtta atactattct
     6301 ggcacaaaaa ccagaactgt ctgcgcagct gactgctatc ggtcaccgta tcgtacacgg
     6361 cggcgaaaag tataccagct ccgtagtgat cgatgagtct gttattcagg gtatcaaaga
     6421 tgcagcttct tttgcaccgc tgcacaaccc ggctcacctg atcggtatcg aagaagctct
     6481 gaaatctttc ccacagctga aagacaaaaa cgttgctgta tttgacaccg cgttccacca
     6541 gactatgccg gaagagtctt acctctacgc cctgccgtac aacctgtaca aagagcacgg
     6601 catccgtcgt tacggcgcgc acggcaccag ccacttctat gtaacccagg aagcggcaaa
     6661 aatgctgaac aaaccggtag aagaactgaa catcatcacc tgccacctgg gcaacggtgg
     6721 ttccgtttct gctatccgca acggtaaatg cgttgacacc tctatgggcc tgaccccgct
     6781 ggaaggtctg gtcatgggta cccgttctgg tgatatcgat ccggcgatca tcttccacct
     6841 gcacgacacc ctgggcatga gcgttgacgc aatcaacaaa ctgctgacca aagagtctgg
     6901 cctgctgggt ctgaccgaag tgaccagcga ctgccgctat gttgaagaca actacgcgac
     6961 gaaagaagac gcgaagcgcg caatggacgt ttactgccac cgcctggcga aatacatcgg
     7021 tgcctacact gcgctgatgg atggtcgtct ggacgctgtt gtattcaccg gtggtatcgg
     7081 tgaaaatgcc gcgatggtcc gtgaactgtc tctgggcaaa ctgggcgtgc tgggctttga
     7141 agttgatcat gaacgcaatc tggctgcacg tttcggcaaa tctggtttca tcaacaaaga
     7201 aggtacccgt cctgcggtgg ttatcccaac caacgaagaa ctggttatcg cgcaagacgc
     7261 gagccgcctg actgcctgat ttcacaccgc cagctcagct ggcggtgctg ttttgtaacc
     7321 cgccaaatcg gcggtaacga aagaggataa accgtgtccc gtattattat gctgatccct
     7381 accggaacca gcgtcggtct gaccagcgtc agccttggcg tgatccgtgc aatggaacgc
     7441 aaaggcgttc gtctgagcgt tttcaaacct atcgctcagc cgcgtaccgg tggcgatgcg
     7501 cccgatcaga ctacgactat cgtgcgtgcg aactcttcca ccacgacggc cgctgaaccg
     7561 ctgaaaatga gctacgttga aggtctgctt tccagcaatc agaaagatgt gctgatggaa
     7621 gagatcgtcg caaactacca cgctaacacc aaagacgctg aagtcgttct ggttgaaggt
     7681 ctggtcccga cacgtaagca ccaatttgcc cagtctctga actacgaaat cgctaaaacg
     7741 ctgaatgcgg aaatcgtctt cgttatgtct cagggcactg acaccccgga acagctgaaa
     7801 gagcgtatcg aactgacccg caacagcttc ggcggtgcca aaaacaccaa catcaccggc
     7861 gttatcgtta acaaactgaa cgcaccggtt gatgaacagg gtcgtactcg cccggatctg
     7921 tccgagattt tcgacgactc ttccaaagct aaagtaaaca atgttgatcc ggcgaagctg
     7981 caagaatcca gcccgctgcc ggttctcggc gctgtgccgt ggagctttga cctgatcgcg
     8041 actcgtgcga tcgatatggc tcgccacctg aatgcgacca tcatcaacga aggcgacatc
     8101 aatactcgcc gcgttaaatc cgtcactttc tgcgcacgca gcattccgca catgctggag
     8161 cacttccgtg ccggttctct gctggtgact tccgcagacc gtcctgacgt gctggttgcc
     8221 gcttgcctgg ctgccatgaa cggcgtagaa atcggtgccc tgctgctgac tggcggctac
     8281 gaaatggacg cgcgcatttc taaactgtgc gaacgtgctt tcgctaccgg cctgccggta
     8341 tttatggtga acaccaacac ctggcagact tctctgagcc tgcagagctt caacctggaa
     8401 gttccagttg acgatcacga gcgtatcgag aaagttcagg aatacgttgc taactacatc
     8461 aacgctgact ggatcgattc tctgactgcc acttctgagc gcagccgtcg tctgtctccg
     8521 ccagcgttcc gttatcagct gactgaactt gcgcgcaaag cgggcaaacg tatcgtactg
     8581 ccggaaggtg acgaaccgcg taccgttaaa gcagccgcta tctgtgctga acgtggtatc
     8641 gcaacttgcg tactgctggg taatccggca gagatcaacc gtgttgcagc gtctcagggt
     8701 gtagaactgg gtgcaggcat tgaaatcgtt gatccagaag tggttcgcga aagctatgtt
     8761 ggtcgtctgg tcgagctgcg taagaacaaa ggcatgaccg aaaccgttgc ccgcgaacag
     8821 ctggaagaca acgtggtgct cggtacgctg atgctggaac aggatgaagt tgatggtctg
     8881 gtttccggtg ctgttcacac taccgcaaac accatccgtc cgccgctgca gctgatcaaa
     8941 actgcaccgg gcagctccct ggtatcttcc gtgttcttca tgctgctgcc ggaacaggtt
     9001 tacgtttacg gtgactgtgc gatcaacccg gatccgaccg cagaacagct ggcagaaatc
     9061 gcgattcagt ccgctgattc cgctgcagcc ttcggtatcg aaccgcgcgt tgctatgctc
     9121 tcctactcta ccggtacttc tggtgcaggt agcgacgtag aaaaagttcg cgaagcaact
     9181 cgtctggcgc aggaaaaacg tcctgacctg atgatcgacg gtccgctgca gtacgacgct
     9241 gcggtaatgg ctgacgttgc gaaatccaaa gcgccgaact ctccggttgc aggtcgcgct
     9301 accgtgttca tcttcccgga tctgaacacc ggtaacacca cctacaaagc ggtacagcgt
     9361 tctgccgacc tgatctccat cgggccgatg ctgcagggta tgcgcaagcc ggttaacgac
     9421 ctgtcccgtg gcgcactggt tgacgatatc gtctacacca tcgcgctgac tgcgattcag
     9481 tctgcacagc agcagtaacc tcgtcatcat ccgcagcttt gcgctgcgga tatctgaacc
     9541 ggaaatagtc actatttccg gttttttatt ctcttaattt gcattaatcc tttctgatta
     9601 tcttgcttaa ctgcgctgca tcaatgaatt gcgccatctc actttgcata cttaccactt
     9661 tgttttgtgc aagggaatat ttgcgctatg tccgcaatca ctgaatccaa accaacaaga
     9721 agatgggcaa tgcccgatac gttggtgatt atcttttttg ttgctatttt aaccagcctt
     9781 gccacctggg tagttccggt ggggatgttt gacagtcagg aagtgcagta tcaggttgat
     9841 ggtcaaacaa aaacacgcaa agtcgtagat ccacactcat ttcgccttct gactaacgaa
     9901 gcaggcgaac ctgagtatca ccgcgtacag ctgttcacga cgggcgatga acgcccgggc
     9961 ctgatgaact tcccgtttga agggttaacc tcaggatcga aatacgggac agccgttggc
    10021 atcatcatgt ttatgctggt gattggcggc gcgtttggca ttgtgatgcg tacaggaacc
    10081 attgataacg gtatcctggc gcttattcgc catacccgtg ggaatgaaat tctctttatt
    10141 cctgcgctgt ttattctgtt ttcacttggc ggcgcggtat ttggtatggg ggaagaggcc
    10201 gtcgcctttg ccattatcat cgcaccgcta atggtccggc tgggctatga cagtattacc
    10261 accgtcctgg tgacctatat tgccacgcaa atcggttttg ccagttcgtg gatgaacccg
    10321 ttttgtgtgg tcgttgctca ggggattgcc ggcgttccgg tgctttctgg ctccgggttg
    10381 cgcatcgtgg tgtgggttat cgccactctg attggcctga tctttaccat ggtgtacgcc
    10441 tcacgagtga aaaagaatcc tcttctgtca cgcgtgcatg agtccgaccg cttctttcgt
    10501 gaaaagcagg cggatgttga acaacgtccg tttacctttg gtgactggct ggtattgatt
    10561 gtcctgaccg ccgtaatggt ctgggtgatt tggggcgtga tcgttaatgc ctggtttatt
    10621 ccagaaattg ccagccagtt cttcaccatg ggtctggtga ttggcatcat cggcgttgtt
    10681 ttccgcctta acggtatgac ggttaatacc atggcttcat ccttcaccga aggggcgcga
    10741 atgatgatcg cccctgccct gctggtgggt ttcgccaaag ggattttgct gctggtcggt
    10801 aatggtgaag cgggtgatgc cagcgtgtta aatactatcc tcaacagcat tgccaatgcc
    10861 attagcggtc tggacaacgc ggtcgcggcc tggtttatgt tgctcttcca ggcggtattt
    10921 aatttcttcg tgacgtccgg ttctggtcag gcggcgttaa ccatgccgtt actggcaccg
    10981 cttggcgatc tggtcggtgt taaccgtcag gttaccgtgc tggctttcca gtttggtgat
    11041 ggcttcagtc acatcattta cccgacctca gcttcgttaa tggcgacgct cggtgtttgc
    11101 cgggtggact tccgtaactg gctgaaggtg ggcgcgaccc tgcttggact gctgtttatt
    11161 atgtccagcg tcgtggtgat cggcgctcag ttgatgggct accactaaaa atgttaagag
    11221 ccgcaattgc ggctcttttt tcattctgcc gtttcagtct ctacggcttc atttttggca
    11281 ttgcgcttca tccacaacgc tagcgctttc agcgagtctg gagtgaactc atcgcagcgt
    11341 gcggtgattt cttccggcgt cagccagcaa acttcactga cttcatcttc ctgtagggcg
    11401 aagggaccgt gagagacgca gctgaacaat gcgccccaga cacggcaatt tttatcttcg
    11461 aaatagaact gcccgtgctc ggcaaagggg acaccggcaa tgcccaactc ttcttccgct
    11521 tcgcgacgcg cggattccag cagttgctca tcggcctgga ctacaccgcc agcggtcgca
    11581 tctaacatgc cgggtaaaaa gtcttttgtc tcggtacgac gctggaccag aattttgccc
    11641 atgccatcat gcacgacgat gtaagttgca cgatgacgca gacactgtgc ccgcatttgc
    11701 tcccggctgg cttgtgcaat gacttcgttc tcttcattga caatatccac ccattcagta
    11761 cttgccaaac gacgctgttc cattatcagg aaaccttctt tttctggcgc tcttacggcg
    11821 cattttgagt tgtgggtaaa ttacggatta atcgcgacct gcgcaatgat actttgatca
    11881 ttgagtgcga ttacactaag tacgtcatta tccagcatgc cataactcgc cggattaccg
    11941 cctttcggaa tactcaccga gccggggttg aagtggaaaa tctccccccg ctgttctgct
    12001 actggcagat gggtatgacc gtaaaccagc acatcgttct ggtttaaagc aggcagattt
    12061 tccgggccaa aaagatgacc atgcgtcaaa aacagacgtt gtttttccag taatacctgt
    12121 tgccacggcg cggttatcgg gaaatgcagc agcatttgat ccacttcgct gtcgcagttg
    12181 ccgcgcacag cgataacctt atgtgccact tcattaagcc gttcagcgac tttggctggc
    12241 gcgtaaccct ccggtaaagc attacgcggg ccgtgattca acacgtcgcc aaggatcacc
    12301 agccactggg caccgctttg ggcaaacaac tccagaacac gttccgtcgc cggtaacgac
    12361 ccatgaatgt ccgatgcaaa catcagtttc atcactcact cctcgtcgaa aaaacgtaag
    12421 ctatgatact ggattctgtc gttgctatca gccagattgc ttcgctgaaa gtgcaataaa
    12481 acgctggaca gacgctcgct gccactggaa tgttgcataa tccaccaggc attctggcac
    12541 ctcatcgcca tgtagtacca ggcggttaat cattagcgcc agatcagtat cagcaatgca
    12601 ccattcacca aataaattcg gctgacccag tactaacaaa tgttctgcca tcgcgaacag
    12661 tttctctgca ctggcttttc cctcggccgt tagtggcgct tttttcgccc ccgcaaagac
    12721 aacatctgtc ggacgctctt cgcggatggg catcagatcg ctgcgcaacc aggcctgaat
    12781 ctgtcgtgca cgcgcacgat tttctaaatc aagcggataa atacgttccc aggtcggtgg
    12841 cgcaaatcga tcttccagat actccgcaat ggcagaagat tcactcaact caaaatcatc
    12901 gatttgtaat aacggcacac ggcgtgtctg accgtaacct tgccacgtcg gttgcaaatg
    12961 ttcaccgctg tcgaggtcga tggtcttgat atgaaacaac aggccttttt cctgcaacgc
    13021 cacccaagcg gataacacat aaggggagaa aaaatgggca tctgaccaaa gcgtgatagc
    13081 gggtttactc attatgtcct cggcaataaa gggggctggc tatcaaacta tagagccttt
    13141 ttgtcactgt cacgtgacga atactcacgt aagtcgccgc gctttctata attgtggtgt
    13201 tcagatgatt agacaaccgg agctccaatg atcgatctct atttcgcccc gacacccaat
    13261 ggccacaaaa ttacgctgtt tctcgaagaa gcagggctgg attatcgctt gataaaggta
    13321 gacctgggga aaggcggtca gtttcgcccg gaatttttgc tcatttcgcc taacaacaaa
    13381 attccggcaa ttgttgatca ttctccagcc gatggcggcg aaccgctaag cctctttgaa
    13441 tctggtgcca ttttgttgta tctggctgag aaaacggggc tctttttgag tcatgaaacg
    13501 cgtgaacgcg ccgccacatt acagtggtta ttctggcagg taggcggact ggggccgatg
    13561 cttgggcaaa atcatcattt taatcacgca gccccccaaa ccattcctta cgctattgaa
    13621 cgttatcagg ttgaaactca gcgtctgtac catgtactga acaagcggct ggaaaactcg
    13681 ccctggctgg gaggcgagaa ctacagcatt gcggatattg cctgctggcc gtgggttaat
    13741 gcctggactc gccagcgaat agacctcgct atgtatccgg cagtcaagaa ctggcatgag
    13801 cggatccgtt cgcgccctgc caccgggcag gcactgctaa aagcacaact cggtgatgag
    13861 cgttcggata gttaacagaa acaggttctc gtgtattatt tcatcctaag taaaacaacg
    13921 gagaacctgc aatggcacaa cctgccgcta ttattcgtat aaagaacctt cgtttacgta
    13981 catttatcgg aattaaggaa gaagaaatta acaaccgtca ggatattgtt atcaatgtga
    14041 cgatccacta ccccgccgat aaagcgcgca ctagcgaaga tatcaacgat gcgctgaatt
    14101 atcgcaccgt aacgaaaaac attattcagc atgtagagaa taaccgtttc tctttgctgg
    14161 aaaaattaac tcaggatgtg ctcgatatcg cacgtgaaca tcactgggtg acgtatgctg
    14221 aagtggagat cgataaactg cacgcgctgc gctacgccga ttcggtatcc atgaccttaa
    14281 gctggcagcg ttaaccgcca tatcgggagg ctgcatgaac atagtgatca ccggagggac
    14341 gggattaatt ggtcgccatt tgattccacg tttgctggag ctgggccatc aaatcacggt
    14401 aatgacgcgt aacccgcaga aagccagttc cgttctcggc cctcgggtga cactatggca
    14461 agggcttgcc gatcaaagca acctcaacgg cattgatgcg gtaatcaacc tggccggaga
    14521 accgattgct gataaacgtt ggactcacga gcaaaaagag cgtctctgcc aaagccgctg
    14581 gaatatcacg caaaaactgg tcgatttgat taatgccagc gacacgccac cgtcggtact
    14641 catttccggc tcggcaacgg gctattatgg cgacttaggt gaagtggtgg ttaccgaaga
    14701 ggaaccgccg cataacaaat ttacccataa actctgcgcc cgctgggaag aaattgcctg
    14761 tcgggcgcaa agtgacaaaa cgcgagtgtg cctgctgcgt accggcgtag tgctggcacc
    14821 ggatggcggt attctcggta aaatgctgcc gccgtttcgt cttggcctgg gcgggccgat
    14881 tggttccggt cggcagtatc tggcctggat tcatatcgat gatatggtca acggcattct
    14941 ctggctgctg gataacgagc tgcgcgggcc atttaatatg gtttcgccct acccgatacg
    15001 caatgaacaa tttgcccatg cgctcggtca tgcgctgcat cgcccggcta ttttgcgcgt
    15061 ccccgcaacc gccattcggc tgttaatggg cgaatcttca gtactggtat taggtggaca
    15121 acgcgcgctg cctaaacggc tggaagaagc gggttttgcg tttcgctggt acgatttaga
    15181 agaggcgctg gcggatgtcg ttcgctgatg tggtttactg caaacatccg ccagttaact
    15241 cccggtgtta cagggttagt ggctttgcgc gataagatcg tctggtgaaa gtcgggtcag
    15301 cgtcaggatg acttcgtggt caaatccttt ttccagcatc tcctgagcaa tacgcagagc
    15361 ttcttcgtgt ttgccctgca ttgcgccttc ttcacgtaat ctgtcagcaa tggtcatcag
    15421 tttctccttt tcttgtggtg cgcgttctgt tatctcacca ataaatgcac ggaaacgctg
    15481 ggcatctccc gtttgtaata cataattaaa cagggctttt agctgtctgt cattagtgtt
    15541 ccctgtaact agcagcgaaa caatttggtc gactaatccc aacagatcgc gctgacgaat
    15601 atgtttctga attaactcca acagcgccat tttacgatgt tgcataatct cgtcatccgg
    15661 caccacggta atatccacca acggaaaagc cgatgaatat attttacggg caatcgcagg
    15721 ctcggcaaat tcatcaagcc agcagagtga ataaggataa ggacttctgc aaccatgata
    15781 aaacagcatc gggatcacca atggaagctc tttatagccc acatcaagat ggttttgcat
    15841 tgccgcaatg gaataacgca tcatgcgaaa agccattaat tcttccggct tactttggtg
    15901 ctctatcact acataaagat aacccactcc ctcctgcgtt ttcacagacc acaagaggtc
    15961 ggaataatat tgccgcaggt cttcatcaat aaaactgttt ggttccagtt taagcgtcgt
    16021 taaatcacac agtttgcgca gcggcgcggg aagatgaata tcaataaaat cccgcgcggt
    16081 gtctggatgg cgtaaaaaag atttaaatac cgcatcatgc ggcgtggaag ttgtcgatat
    16141 tgtcatggcg ttccgtcacc cctcaaattt aagatgacgc gacaataacc acgattaaca
    16201 cccaatgcag caggcagttt ttatctttgg acagcgaatt cagaagtatt tttagcggac
    16261 tgcaactttt acatttcagc ggaagctgac tcgcaaattc cccttgaaaa tactccggca
    16321 gcgttagcca ccggagaggc aattatttca gcgaaccttt aaggaactgt tgcagacgag
    16381 ggctttgcgg gttgccaaat aactgctccg gcgcgccttc ttcctctatt ttcccctgat
    16441 ggaggaaaat gacgtgagta gatacatggc gagcaaagcc catttcgtgg gtcaccacca
    16501 ccatcgtttt tccctcttcc gccagctgct gcataatgcg cagcacttca cccaccagct
    16561 ctgggtcaag ggccgatgtc ggttcatcaa acaacagaac ttccggttcc attgccagcg
    16621 cccgcgctat ggaaacacgc tgttgctgac caccggacag gtgcaccggg tatttcccct
    16681 gcgcacgttc gtctatcccg acttttgcca gatacttcac cgcccgctcg cgcgcttcct
    16741 gcttgctcag gcccaacacc tgaatcggcg cttccatgac gttttccagc accgtcatat
    16801 ggctccagag attgaagtgc tggaatacca tcgtcaggcg tgtgcgcagt aagcgcagtt
    16861 gatttttatc ggcgactttg agttgaccgt ctttgtcgcg caccagattg atcgtctggc
    16921 cattgaccac gatcgaccct tcactcggtt tttcgaggaa gttaatgcag cgcagaaagg
    16981 tacttttccc cgatcccgac gatccgatga tgcttattac atctccggca ttcgcttgca
    17041 gtgacacccc tttcagcact tcgtgttcgc catagcgttt gtgcaaatcg ataacgttta
    17101 atttattctc ggacatcgta ttctcagtgc gttgaagaag gtttcacatg ctgcaaccag
    17161 cgtttttccg ctctgcgaaa gaggctgatc aggacataag agatgattaa atagagcacc
    17221 gcggcaatgc cgaaggcggt aaaaggttga tacgtggcgg cgttaatatc gcgggctatt
    17281 ttcagcagat ccggcaccgt ggcagtaaat gccaacgcag tagagtgcag catcaggatc
    17341 acttcgttgc tgtatgccgg taacgcaata cgcagcgcag aaggcaaaat aatgcagcga
    17401 tacattttaa aagtcgagaa gccataggct ctggcggctt caatttcccc atgcggcacc
    17461 gaacggattg ccccagcaaa aatctcggta gtgtaagcgc aggtgttaag cgtcagcgcc
    17521 agcacggtac agttcaggcc actgcggaaa aaggcgttaa gaaactcggt ccccttaaca
    17581 atctccagcg tatacatgcc ggaatagaac accagcaact gaacatacag cggcgtaccg
    17641 cgaaaaatat aggtaaataa ccagattgga aactggatgt atttattact ggagacacga
    17701 ccaatcgcca gaaacagcgc caggactccg cctatcacta ccgacaaaat aagcagccac
    17761 agagtgatcg ccacaccagt aaagcgataa ccgtcggtcc acagcagcgg tttccagtat
    17821 tcatgtaaga tttcgatcac aggtcagccc tcttcacacc cacagagtag cggcgctcaa
    17881 ggaacagcag cacaccattg gaaacggtgg tgaaaaccag gtaaatcacg ccacagacga
    17941 tagcgaaata gaacggttcc caggtacttt tgcctgccag ctgcgtggct ttgaccacat
    18001 cttccaggcc gagtaacgaa accagtgcgg tagatttgag gatcacctgc cagttgttgc
    18061 caatgcctgg cagggcgtaa cgcatcatcg ccggaaacat gatccgccga aacacttgcc
    18121 cacgagtaaa accgaacgcc ctcgccgcct ctatatgtcc tttcggcact gccataaaag
    18181 caccacggaa cgtttcggta aaataagcac cgtagataaa accgagagtg ataataccag
    18241 cgaccatcgg atcgatatca atctgcccga cgcccatcgc ctccgtcacc gtgtttagcg
    18301 caatctgcaa accgtagaaa atcagcagca tcaacactaa gtccggcacg ccacgaatca
    18361 gcgtggtgta cccttcgaaa atcagccccg aaagccgatt ttgcgagagc ttaccgccag
    18421 cgccaattaa accgatgatt acagcgagca ctacagagct gatagccagc tccagcgtga
    18481 cgagcgcacc ctgtaaaata acacctgaaa acccatacaa catgctgcct gtcctgtcgt
    18541 gtgtggtgaa ttaccgcctc ttgtctccct ccggttaccc gaagggagcg ggctttcttt
    18601 caccgatggc gattaaccac cataaacatc aaaatcgaag tactttttcg ctaatttctc
    18661 gtaagtaccg tcagcgcgca tttcggcaaa ggctttgttc agtgcctcgc gcagttcgtt
    18721 atcttcttta cgcaggccca tgccggtccc tacgccaaac agtttttcat ctttaacaga
    18781 cgggccaccg aatttgtaat ctttaccgac aggttgtttg aggaaacctt cgctggcagc
    18841 gacctcatcc tggaacgcgg catcaatacg tccggcagtc aggtcagaat aaatgttgtc
    18901 ctgcccctga tacgagacga tttcaatgcc ttttggtgcc caatgttcat taccgaacgt
    18961 ctcctgggta gtgccctgca atacgcctac ccgtttgcct ttcagcgact cgactgtcgg
    19021 ctgaatgtca gaatttttcg ccaccaccag acgagaatcg gcagcgtaca gtttgtcggt
    19081 gaaggctatt tcttgctgac gtttttccgt aatggaaagc gatgacatga tggcgtcaat
    19141 cttcttcgct tttaaggacg ggattaacgc atccagcgga ttttcgacaa acgtacattg
    19201 cgtattgatg cgtttgcata attcctttgc cagatcgata tcgaagccaa ccagttcgcc
    19261 ttgtgaattt tttgattcaa atggcgcata ggtcgggtcg gtaccgatgc ggatgttttg
    19321 cggaatcgca gcaaatgccg cagttgcgct ggagaaggcc agaaccagag agagcgatag
    19381 caccagtttt ttcataatgt cctcaactga cagtcttttt atagggaata tttacaggtt
    19441 tgttacactt atcgtgccat aaaattgacc atcaaggcaa aatatcccgc cccgttagac
    19501 gtattatttt tcattaaaaa tgcttaagta tgcatttcaa tgcaccacga tagtgcaaca
    19561 aacctggcgt gcaccatgat gatcattcca tcaggtacag cttccctgcg acgtatcagt
    19621 caccgtagac attaaagtca aaatactttt tcgccatctt gtcgtaggtg ccgtcctgac
    19681 gcagctcgcc aagcgcctta ttgaaggcag ccgtcagttc agcatcatct ttacgtagcc
    19741 ctacaccggt gccatcaccg aagtattttt tgtcttttac tgatgagcca gcaaaggcga
    19801 aatctttacc agcaggttgc ttgaggaatc cttcgctggc agcaacttca tcctgcagtg
    19861 cagcatccag acgtcctgca gccagatcgg aatagaccaa atcctggttg gcataggcca
    19921 ccacatccac acctttgcta cgccaggtct cgttagcgta agcttcctgg gttgatccct
    19981 gcagcacacc aacatgttta cctttcagtg agtccagcgt tggctgaatc ggcgaacctt
    20041 tggcggcaat cagacgggaa tctgcggcgt acagcttgtc ggagaaggca atctcctgct
    20101 gacgtttatc ggtaatggaa agcgacgaaa taatggcgtc gatttttttc gctttcagtg
    20161 aggggatcag cgcgtcaaag tcactggcaa cccaggtaca tttcacctgc atccgtttgc
    20221 acatctcgtt accgagatcg atatcaaagc cgacaaagtc acctttggca tctttcgacg
    20281 agaacggtgc ataggtggta tcggttccga tacgtaccgt ctccggcaat gccgcatagc
    20341 tggaagccgc tgcggaaata ccgactaaca aagacagagc gagaatcgac ttcttcatac
    20401 ataaccctca agtgaaaatg gcttttttat gttgtgtatt gtgttgtgtg tttgcaggct
    20461 tgttcatgca ggtcttatgc catcttgacg acaacagtaa cattcaacgt taaatatgtt
    20521 aataagacgt tgcattattg tcctaaagtt gaagatagca ggtatagcgg ttgaatcgca
    20581 gcgtttcgat tgaatggcag aaacaaaatg tcgaggattt gatcgcggtt gcaaaattgc
    20641 cctgaaacag ggcaacagcg gagttatgcg ccctgccagc gggcaaagag atcttcagga
    20701 agggttatcg caaactggtc aagaacacga ttaaccgtct gatttatcac atcatcaagg
    20761 gattgcgggc gatgataaaa cgccggaacg ggaggcataa tcaccgcacc gatttctgct
    20821 gcctgcgtca ttaaacgcag atggcctaag tgcaatggtg tttcacgcac gcagagcacc
    20881 agtgggcgac gctctttcag caccacatct gccgcacggg tcagtaagcc atcagtatag
    20941 ctatggacaa tgccggacag ggttttgatt gaacagggta aaatcaccat ccccagtgtc
    21001 tggaaagaac cggaagagat gctggcggca atatcgcgcg catcatgcgt gacatcagct
    21061 aatgcctgca cttcgcgcag ggaaaaatcc gtttcgaggg ataaggtctg gcgcgccgcc
    21121 tggctcatta ccagatgcgt ttcgatatct gtgacatcgc gcagaacctg taataagcgc
    21181 acaccataaa tcgcgccgct ggcaccgctg atgcccacaa taagtcgttt cataaatagt
    21241 tgccctgtca gacttgcagg cagactttgc aggatttcgg cgggagttgc aagtcagggt
    21301 gccagaccgg caccctgagc gaaggcatca tccttcgtta tgcatttcga gattttccac
    21361 ttcgttctga cgttgcactg ctttggcgtc gtcattacgt aaagtatcga ggaaatcgag
    21421 gtagccctga tcaacatctt tggtgacgta gacgccgttg aacaccgagc attcaaactg
    21481 ctggatatcc gggttttcag cgcgaacggc ttcgatcaga tcgttcagat cctggaaaat
    21541 caacccgtca gcaccgatga tctggcgaat ttcatctact tcgcgaccgt gagcgatcag
    21601 ttccgtggcg ctcggcatat caataccata aacgttcggg aagcgaattt ccggtgccgc
    21661 agaagcgagg tacactttct tcgctccggc ttcgcgtgcc atctcgataa tctgctcaga
    21721 agtggtgcca cggacgatag agtcgtcgac cagcaggacg tttttatcgc ggaactcggc
    21781 gcggttggcg ttcagtttac ggcgcacgga cttacgacgc agctgctgac ccggcatgat
    21841 gaaggtgcgg ccaacatagc ggtttttaac gaagccctgg cggtacggtt tgcccagaat
    21901 acgagcaatt tccagcgcga tatcacacga ggtttccggg atcgggatca ccacgtcgat
    21961 atccagatct tcccattcgc gggcaatttt ctcgcccagc ttcgtgccca tattcacacg
    22021 cgcgctgtaa acggaaattt tgtcgatgaa cgagtccggg cgagcaaagt atacatactc
    22081 aaacaggcac ggattgctga ccggattgtc agcacattga cgggtaaaca actgcccttc
    22141 ttcagtgatg taaatcgctt cgccaggcgc gacgtcacgc aggaaatcaa agcccagtgt
    22201 atcgagcgca acgctttcgg aagcgaccat atattctgtg cggttctcgt caatatcacg
    22261 ttttcccaga accagcggac gaataccgtt tggatcgcgg aaagcaacca taccgtggcc
    22321 gataatcatc gccacacagg catacgcgcc gcggattaag cggtttgtag cagcgatggc
    22381 ggcgaaaatg ttgtcggctt caagcgggta gtggcggaag ttgtccagct cgctggcgaa
    22441 gatattaagc agaatttccg agtcggaagt ggtgttgatg tggcggcgtt tttcttcaaa
    22501 cagtttttta cgcaactcgt gagcgttggt cagattgccg ttgtgggcaa gcgtaatgcc
    22561 atacggagag ttaacgtaaa acggctgcgc ttcagaggcg ctggagctgc cagccgtagg
    22621 gtaacgcaca tgaccaatgc ccatattgcc ctgcaaacgc tgcatatggc gagcttcaaa
    22681 tacatcgctc accagcccgt tcgctttacg caaacggaag cagttattgg catctatggt
    22741 gatgatgccg gcggcatcct gaccgcgatg ctgaagcacc gttaaggcat cataaatcga
    22801 ctggttaacc ggcataacac cggcgatacc gacaataccg cacatacgtc tttttcctcg
    22861 ttaagccaca tctcagagca cttacgctct gggcaagaaa cttgacgagc tttgcagata
    22921 atcaaaaaac catctgatga taaaactgaa ctgcggaatc agttgtgatt tactccagtc
    22981 ttcgcttttc gacaccccgg taaaggagtc gagaaagaag agaatagcag caacaatcaa
    23041 cacaccgcgc aatgcaccga aacagacgcc cagcacccga tcggtgcctg acaaccccgt
    23101 tttctccacc agcaggccta tcacgaagtt cacgatagcg ccaacgatca gggtggcgat
    23161 aaacaggacc gcgatggcaa tcccatttcg aaccagttcg tcttcaaagc ccgtaaacca
    23221 gactgacagg taagtgtagt aatgactggc aacaaagaaa gcacaacccc atgtcaccag
    23281 cgataacgct tcacgaacaa agccgcggat caggctaacc agagaggaaa aagcaatcac
    23341 cgcgattatg gcgtaatcaa tccagaccat atgtgtccca cgattttacg ccctgtcatc
    23401 ctgttcgggg cgcattctaa cagaaaaaga aaacgtttgc gtagggattt ccttcccgcg
    23461 catcaataaa aatggcgctg aaaaaatatt caacgccatc gactttttat gcctttgcgg
    23521 catcggacaa tgcgtgtcgg atgcggcgta aacgtcttat ccgacctacg gttctacccc
    23581 tgcgtaggcc tgataagacg caccagcgtc gcatcaggca acggctgtcg gatgcggcgt
    23641 gaacgcctta tccgacctac ggttctaccc ctgcgtaggc ctgataagac gcaccagcgt
    23701 cgcatcaggc aacgcctgtc ggatgcggcg tgaatgcctt atccgaccta cggttctacc
    23761 cctgcgtagg cctgataaga cgcgccagcg tcgcatcagg caacggctgt cggatgcggc
    23821 gtaaacgcct tatccgacct acggttctac ccctgcgtag gcctgataag acgcgccagc
    23881 gtcgcatcag gcaagaccgt attaattcgg cgtatagccc attaccacgc cacttaagcc
    23941 agaaagttgc ttcaactcac ccagcgaacc tttcagctta tctttcgagg catccggccc
    24001 aaccagaata cgggtaattt taccctgcac tggcgtggat ggcgacgtat aaacccgata
    24061 accggcaccg cgcagcttac cgacaatctc attcacttta tcggcatttt tcagcgcgcc
    24121 cagttgcaca acataggctt tacccgttgg cgctgctttt tcttccacga ccggcttcgg
    24181 ttctggcttc ggcgcaggtg gtgcttcaac cttcggctta ggcggctcca ccggtttcgg
    24241 cttcggtggg acgaccggtg caggttccgg ttcaaactcg gtgttattgg ctgcaatagt
    24301 ggccggatcg agcgacggtg ctgcggcatc acctgcccgc acctcttccg ctgcgccttc
    24361 cggcggttgc gtcggcaacg cctgggtggc ggctggcatc atatcaggct catcgcgatc
    24421 gcccgctttt ggcaccagcg ggatagccgc aaactcatcc tgataatgtt ttttctgccc
    24481 gtccagcagc cctggaagta caatcacccc cagcgccacc agcacgatcg tgcccactaa
    24541 ccgattctga aacttacttg ccaccgcttc tcctcgcgtc aagcacttcc atgacatgtg
    24601 cgaccgtgtg gaacgaacca cacaccagca cggtgtcttc cgctttagcg tccgccattg
    24661 cggcatccca tgcctgcgca acgctatcaa atgatttgcc gttacccaaa tgctcaagca
    24721 gttgttctgc cgtggcaccg cgcggccctt ccagtggcgc acaataccag tcatcaacca
    24781 cgcttttcaa ccaggccaga gttccggcaa tatctttatc atgtagcata ccgataaccg
    24841 ccagcacgcg cccgtttttc ggtagcgttt tcatacgccc ggtgagatat tccgccgcat
    24901 gtggattatg cgcgacatca aaaataacgc gtggcgactc gctcacaatc tggaaacgtc
    24961 ccggcaaaat tgcgctggca atcccgtcgc gaatggcatt ttcactgacc tccagcccgc
    25021 tggcacgcag tgccgccagc gccgttgcgg cattcggttg cgggacaagc ggcaacggca
    25081 ggttttccag cgtgccgtga gcatcgctaa acgcccagtc atgatcggtg acggaatagt
    25141 tccactcaac gccccgacgt tgtaacagtg cacctttttc ctgcgccaca tcagcaatgg
    25201 tagaaggcat ttccggctca ccgacaattg ccggtttttc gctgcgaaag atgcctgctt
    25261 tctcgcgacc aatactttcg cgatctggcc ccagccagtc ggtatgatcc agcgcaatac
    25321 tggttactac cgcgacatcg gcgtcgacaa tattggttgc gtccagacga ccgcccagcc
    25381 ctacttccag aatcaccacg tcaagttgtg cctgtttgaa cagccacaac gccgacagcg
    25441 taccgtactc gaaataggtc agggaaatat caccgcgtgc cgattcaatc tccgcaaaag
    25501 aggcggtgtg ggccgattcc ggcaattcct gaccctgcac acgtacgcgc tcggtataac
    25561 acaccagatg aggcgaactg tagacgccca ctttgtaccc tgccgccatc agaatcgact
    25621 ccagcgtacg gcaggtggtg cctttgccat tcgtacccgc aacggtaaac acaaatggcg
    25681 ctggtttcag aacgccaaga cgcgccgcga cctggctcac gcgctcaagg ccgagatcga
    25741 tagttttact gtgcaggttt tccagataag aaagccacga agccagaggc gacgcggctt
    25801 gaggagtgcg tttgataatc atggtatccg ctgattcgtt acggtgagaa tagcaaaagg
    25861 gcagagccag tggccctgcc cttatcagtt atcaggcctc aggttcctga tccggtaccg
    25921 ggggtaccac tacgccttca cgcggcgctt caggattcgg cgctggcaga ttcatcaact
    25981 tcgccagaat gctcgccagt ttcaggcgca tttccggacg acggacgatc atgtcgatcg
    26041 cgcctttctc gatcaggaat tcactgcgct ggaatcctgg cggcagtttt tcgcgaacgg
    26101 tctgttcgat aacacgcgga ccggcaaagc cgattaacgc tttcggttca gcgatgttga
    26161 gatcgcccag catggcgaaa cttgcagaaa caccgcccat cgtcgggtcg gtcagcacgg
    26221 agatgtacgg caagccgcgc tcctgcattt ttgccagtgc cgcagaggtt ttcgccatct
    26281 gcatcagcga catcagtgct tcctgcatac gtgcgccacc agaggcggag aagcagatca
    26341 gcgggcagtt atcttccagc gcctgctcaa cggcacgcac gaaacgtgca cccacaacag
    26401 accccattga accgcccata aaggcgaact cgaatgccgc agcgacaacc ggcattccat
    26461 acagagtgcc tttcatcacc accagcgcat ctttttcgcc ggtttctttc tgcgcagatg
    26521 ccagacggtc tttatacttc ttggagtcac gaaacttcag cacatctttc ggctcaagct
    26581 cgctacccag ctccacaagg cttccttcat ctaacaggct atgcaggcga ttacgcgctg
    26641 tcatacgcat gtgatggtca cacttcggac agacctcaag attacgttcc agctcagcgc
    26701 ggtataaaac ctgaccgcag ctatcacact tagtccacac cccttcagga atgctcgcct
    26761 tgcgggtggg agtaatgttg cttttaattc gttcaatcca gctcattagg gacctttctg
    26821 tctgaacctg gttcgatgcc agttttatct ttggggacgc ataatgccat ttttgccccc
    26881 aacagaccat gaatgttgca cattaaaaca taacagcccg aaactttgga taaaaaagtg
    26941 gtcgaaccgc ggagttactt tttattttgc ggcgcgtgcc gcagcgcgtt tgtgacggat
    27001 tatttcgatg acgcccggca aaatagaaac cacaataatc ccgacgatca gcagcttaag
    27061 gttatcctga accatcggga ttgtaccgaa gaaatagcct gcgtaggtaa aaagcagtac
    27121 ccacaacagt gcgccgatca cgttataagc agcgaaatga cggtacgaca tgtggcccat
    27181 tcccgcaaca aacggggcga acgttctgac gatcggcaca aaacgggcga gaataatcgt
    27241 tttgccgcca tgtttctcat aaaactgatg ggttttatcg agataactgc gacggaaaat
    27301 tttcgaattg ggattactga ataacttttc accgaacaag cgcccgatag tgtaattgac
    27361 cgcgtcaccc acaatcgcgg cgatcagcat cagcaccacc atcatatgga cgttgagatc
    27421 gttggtttcc agtgatgcca acgctccggc aacaaacagc aatgaatcac ccggtaaaaa
    27481 cggcgttacc accagaccgg tttcacagaa caaaattaag aacaaaatgg cataaaccca
    27541 gacgccgtac tccgcgacca gctctgccag atgcacgtca atgtgcagga taaaatcgat
    27601 gaggaaataa atcaggtcca tatttgccta tgccttgtac tcgtcatctt tcaggctgta
    27661 actacgttgg ctacagcctg aaatgttccg agcattattc tcgttagtcc gccagaaata
    27721 gcgggcccat tggcggtttt ggaagatcat accggtcagg gtaatccacc gcgaccagat
    27781 acagcccttc cgcttttgcc gttgctgccg ccagcgttct gtcctttgcc gccagcaact
    27841 ctgctatcca gctctccggc tggttgtggg caccgacttc catcaggctg ccgacaatat
    27901 tcctgaccat atgatgtaca aaggcattcg ctttgatatc aaccaccaca taaggaccgt
    27961 gacgcgtgac attaatatgc ataacgttgc gccacggggt tcgggactgg cactgcaccg
    28021 cacggaacga ggtgaaatca ttctcgccca gcaagcactg cgcagcccga tgcatccgtt
    28081 cagcgtccag cggttcgtaa aaatgggtta cccctttact cagtaccgcc gggcgcagcc
    28141 gatgattgta gatgatgtag cgataacggc gagccgtggc gctaaatcgg gcatgaaaat
    28201 catcaggtac agctttaacc caacgcacgg cgatgtcacc aggtaaattc gcatttacgc
    28261 ccaacgtcca cgcagcgtct ttgcgctgcg cggtagtttc gaaatgcaca acctgcccgg
    28321 taccgtgtac ccctgcgtca gtacgcccgg cgcagaagac ggtgatgggt tcgttcgcca
    28381 cctgggagag cgccttttcc agcttctcct gcacactgcg gacttcattc tgccgttgcc
    28441 agccgtaata cttactgccg tcgtactcaa tgcccagcgc aattttataa actggcggtt
    28501 gttgctggtc ggacattagt acagatactc ctgcaccagt ttctcggcga tttttactgc
    28561 catcagcgcg ccgccaaagc gaacgttatc ggccaccgac cagaactgga cttgctccgg
    28621 cataccgtag tcattacgca cgcagccaac agaaagatgc ggcgtacccg aagcatcacc
    28681 tacctgagtt gggaattcgt tctcttcaga gagcacaata tcttcccctt gaacaaacgc
    28741 atcacgcgct tcttctgctg ccagtggacg cagagcttca aagttgacca tctgggcatg
    28801 accgtagaat accggtgcct ggacgacgct tgccgaaatc atcagtcctt cgtcctgcag
    28861 gattttgcgt acttcgtcaa cgatacgacg ttcttcacgc acgctacctt cgctatccgg
    28921 cagtaacggc agcatgttga acgcgagctg acgcccgaag aaatcttctt cgtcaatcgg
    28981 aatgccgttg agcaatttcg cactctgccc cgctaacgca tcgaccgctt ttttgccctg
    29041 ggcggaggct gaaatcaggc tggtaacact gatacgcgac aaaccgccct gatcgattaa
    29101 cggtttcagt gccgccagca gctggctggt cagactgtct ggtacggcga tgacattccg
    29161 gttgcggtaa tcggtcagta caaacgggtt tacttccggc accaccagcg gtacgtcggg
    29221 ttcaagagca aacaatccgc tgctgtcgat caccaggcaa cctgagttgg tcgcttcttc
    29281 aacccaggct gcggtagctt ctttgcctgc gacaaaaaat gccagctgcg cctgcgtcca
    29341 gtcgaattca gcagcatcct gcacggtgat tgtcttacca ccaaagcgca attgttcgcc
    29401 tgcgctttcg ttacgtgcca gtgcataaat ttccccaacc gggaactgac gttcagccag
    29461 cgtttcaagc agggcttcgc ccacagcgcc agttgtgccc aggacggcaa tgttccagcc
    29521 ttcagacatg gtggtttact ccagaaatag caaagctccc tgccaatgtt atagcagaga
    29581 gcatgaagaa gagattaacg tgccggatga tgaacggcgt taaaacccag tttacacagc
    29641 aatgatgccg cactggcgtc atcacaaatt acatacagag atgaccattc acggcgctca
    29701 agatagtttt tgcgcagttt atcgaactca cccggtatcc cggcgacttt acgcagcggt
    29761 gcgtcatcgc ggcgcacatc atacaccaaa tgcaccagcc ttttcagcgt cggttgatcg
    29821 agcggtccat gcagagtaat gcgaccaaac tctggcgcag gcagtaatgt atccagcgca
    29881 acgtgctgtt catgcccaat aaacttgcta taggcttcaa acacttgcgt agtaccgcgt
    29941 gctttacctt ccagggtata gcctgcaata tgcggcgtgc cgatatccac ttttgtcagc
    30001 aattccacat ttagttccgg ttcgccttcc cagacatcca gcactacgct taacttctgg
    30061 ccttcattca ggcaggtcag cagcgcggta ttatcgacga ctgcgccacg gcaggcgtta
    30121 atcagaatcg ctccgggctt caggctacgg atcagttttt catccgccag atgcagcgtt
    30181 ttgtacggcc cgtctttgaa gagtggcgta tggaaagtca gaatatccgc gcgctggact
    30241 aactcatcca gcgagcggaa atcaccctca tccccacagt cggcgcgagg cggatcgcaa
    30301 agtaaggttt taatccctaa cgcttccagt cgtgcctgta aacgccgacc aacgttacca
    30361 acgcctacga tccccacggt acgctcgtgc agtgaaaatc catcgcgttc ggcaagcatc
    30421 agcagggagg agaaaacata ttccaccacc gcaatcgcat tacagccagg tgcagcggaa
    30481 aaaccaattc ccgcctgctt caaccatgct tcatcgacat ggtcggtccc cgcagtggca
    30541 gtgccaacaa atttaatggg ttttcctgcc agcaaagatt cattcacttt cgtgaccgaa
    30601 cgcaccatca gcgcatccgc gtctgccagt tgagcgacgg ggattggacg cccgggaacc
    30661 gcgatcacct cacccaaacg gctaaataag tcgcgggcat aaggcatatt ttcatcaaca
    30721 aggattttca cgtttgtgtt acctgtatga gacgagagtt aaccggacaa gtgtgccata
    30781 atctcgcggc caggcatact tgcgaagatt tcaggtataa ggatacgtaa tgatacaacc
    30841 tatttccggc cctcctcctg ggcaaccacc aggtcaggga gataacctgc cgtctggcgc
    30901 gggcaatcag cctttatcca gtcagcaacg tacttcgctg gaaagcttaa tgacgaaagt
    30961 gacctcactg acgcaacagc aaagagcaga actgtgggcg ggtatcaggc acgatattgg
    31021 tctgtcggga gattcaccgc tgctttcgcg tcactgccct gccgctgagc ataatctggc
    31081 gcaacgtctg ctggccgcgc aaaaaagcca ttctgcccgc cagcttttag cgcaattagg
    31141 ggagtattta cgtctgggga ataatcgtca ggcggtcacg gattatatcc gtcataactt
    31201 tggtcagacg ccgctgaatc agctctcacc ggagcaatta aaaaccattc tcaccctgtt
    31261 gcaggaaggg aagatggtta ttccgcaacc gcagcagcgc gaggcgaccg atcgtcctct
    31321 attaccggcg gagcacaatg cgctcaaaca gctggtgacc aaacttgcgg cggcaacggg
    31381 ggagcccagc aaacagatct ggcaatcgat gctggaactt tccggggtga aagatggcga
    31441 gttaattcca gcgaaactgt ttaaccatct ggtgacctgg ctgcaggcgc gtcagacgct
    31501 aagccagcaa aatacgccga cgctggaatc actacaaatg acgctaaaac aacctttaga
    31561 tgccagtgaa ctggcggcgt tatcggcata tatccagcaa aaatatggcc tttctgcgca
    31621 atcatcgctt tcttctgccc aggccgagga tattcttaat cagctttatc aacggcgggt
    31681 taaagggatt gatccgcgtg atatgcaacc gctgcttaat ccttttccac cgatgatgga
    31741 cacgttgcaa aatatggcaa cgcgtcccgc gctgtggata ctgttagtcg cgattatcct
    31801 gatgctggtc tggctggttc gttaaccccg acgaaacgac agtatcgtga caataattcc
    31861 cagcaccgca gagatcgccc cggcaagaaa taccgaagag taaccaaacg tggtcgccag
    31921 cattcccgca agcggcccgg agacgccgag ggcgatatcc tgaaacgcgg cgtaaccgcc
    31981 cagtgcggtg ccgcgaactt gtgaggggac gcgtttaacc acctccacgc ccagcgcagg
    32041 aaagataagc gaacatccgg ctccggttaa cgccgcgccc gctaatgcga cccacgcccc
    32101 tggggcttgc cagagcagca acaagcccac cgtttctaca agcagagaga caatcgccac
    32161 tttcacgccg ccaaaacggt ccggcatcca gccaaacatg acgcgcatca cgacaaatgc
    32221 gccgccaaac gcggtaagag taaagcccgc catcgcccat cctttgctgg caaagtagag
    32281 cgaaacgaaa gtcccgataa ccgcaaaacc aacgccttgt agtgccagac ctaaccctgg
    32341 tttccagata agtccgacaa cgctccacag agatggacgt tctcccgcca gggctggtac
    32401 tttgcgcact gtgccgttac aggcccacgc cagtaagggt aatgccattg tggtgagcgc
    32461 cagtgcggca aaaccgtaat ggctatgaat caacaggcca agcggagcac caacagcgag
    32521 ggcaccgtaa atcgccattc cattccatga catcactttg ccagagtgtt ttggccctac
    32581 gatgcctaac ccccaggtca gagcgcctgt cagtaactgg ctttcaccaa acccaagaat
    32641 caaacgcccg atgaccaaca gggcaaattt gaacggtgcg gagacaggca aaatcgccgc
    32701 cagcagcaac gcgccgccag ccagaccaca agctaacatc ccctgaagcg ccgaacgttt
    32761 tgcgccatat tgatcggcca gtcgcccggc atagccacgc gtcagcaccg tagccagaaa
    32821 ctgaatgccg acggcaatgc cgaccatggt attgccatag cccagatcat gatgaacaaa
    32881 caacgggata accggcaacg gcaaccctac ggtcatgtag gtgagaaaaa ccgcaaaagc
    32941 gatgcggaag agcgaaaaat tggcagaaga tcgtgtttcg gtttggctta cagcagtcat
    33001 gcattactcc agaatgcagc gcaaggcgag gagtatcccc gtctcatctc tctggtttca
    33061 gggttacagt gcgttggcag gatttaacgc gtacgtcttt tcagaaggaa atcgacaaag
    33121 cggggagttt gcctggaact ggcggcgatt gtcaatgatg tgaaaaaggg aaccatcagg
    33181 ttcccttttg cgttagtgcc ggaggccgca ttggcgcgta acgtcggatg cgacgctggc
    33241 gcgtcttatc cgacctactc cggcctactg cgaattaatc tttcagcttg cgcattacca
    33301 gcgtggcgtt ggtgccgccg aagccgaagc tgttagacat aacagtggtc agttcgcgat
    33361 cggtcgtttc ggtcacgatg ttcagacccg cagcctgctc gtccagctct tcaatgttga
    33421 tgctcggggc gataaagccg tgttccagca tcagcagaga gtagatagct tcctgtacgc
    33481 cagcagcgcc cagagagtga ccggtcatgg ctttggttgc agaaatcgcc gggctcttat
    33541 cgccgaacac ttcacggata gctgccagct ctttcacgtc gccaaccgga gtcgaagtgc
    33601 cgtgggagtt caggtaatcg attggggtat caacgccatg catcgccatc tgcatacaac
    33661 gtactgcgcc ttcgccagag ggagcaacca tgtctgcgcc atcagacgtt gcgccgtagc
    33721 caacgatttc agcatagatg tgagcaccac gcgccagcgc gtgttccagc tcttcaacca
    33781 ccaccatacc gccgccgcca gcgataacga aaccgtcacg gtgagcatcg taagtacggg
    33841 aggctttttc cggggtgtcg ttgtatttag tagacagcgc acccattgcg tcgaattcgc
    33901 aagccatttc ccagcacagc tcttcgccgc cgccagcaaa cacgatgtcc tgtttgccca
    33961 gttggatctg ctctactgcg ttaccgatac agtgtgcgga agtcgcacac gcggagctga
    34021 tggagtagtt aacgccatga attttaaacg gcgtggcgag gcaggcagaa acgccggatg
    34081 ccatcgcttt ggtgaccaca tacgggccaa ccgctttcag gccgcgcggg ccgcgcattg
    34141 cgtcagcgcc gaacacctgg aaacgcgggg aaccgccgcc agaacctgca atcaggccaa
    34201 cacgcggatt attctggtaa gcttccggag agaggcccgc atctgcgatt gcctgctcca
    34261 tagaaaggaa tgcataaatg gatgcgtcgc tcataaagcg cacaactttg cggtcaatga
    34321 ggccagtggt atccagtttt acgttgcccc agacgtggct acgcatgccg gaatccttca
    34381 gctcctgaga gaaagtgatc cctgaacgtc cttcacgcag agatgccagg acttcctgct
    34441 ggttattacc gatgctggaa acaatgccca ggccagtaat cactgcacgt ttcattcaat
    34501 acctctgtaa gtcgcacata gagtaagttt cgaatgcaca atagcgtaca cttgtacgcc
    34561 gaacaagtcc gatcagccaa ttaatagaga aatttgcgca gccttacaca catcgctaag
    34621 atcgagctac cgcctgtaag acgagtaact tacgtgaaac actactccat acaacctgcc
    34681 aacctcgaat ttaatgctga gggtacacct gtttcccgag attttgacga tgtctatttt
    34741 tccaacgata acgggctgga agagacgcgt tatgtttttc tgggaggtaa ccaattagag
    34801 gcacgctttc ctgaacatcc acatcctctg tttgtggtag cagagagcgg cttcggcacc
    34861 ggattaaact tcctgacgct atggcaggca tttgatcagt ttcgcgaagc gcatccgcaa
    34921 gcgcaattac aacgcttaca tttcattagt tttgagaaat ttcccctcac ccgtgcggat
    34981 ttagccttag cgcatcaaca ctggccggaa ctggctccgt gggcagaaca acttcaggcg
    35041 cagtggccaa tgcccttgcc cggttgccat cgtttattac ttgatgaagg ccgcgtgacg
    35101 ctggatttat ggtttggcga tattaatgaa ctgatcagcc aactggacga ttctctaaat
    35161 caaaaagtag atgcctggtt tctggacggc tttgcgccag cgaaaaaccc ggatatgtgg
    35221 acgcaaaatc tgtttaacgc catggcaagg ttggcgcgtc caggcggcac gctggcgaca
    35281 tttacgtctg ccggttttgt ccgccgcggt ttgcaggaag ccggattcac gatgcaaaaa
    35341 cgtaagggct ttggacgcaa acgggaaatg ctttgtgggg tgatggaaca gacattacca
    35401 ctcccctgct ccacgccgtg gtttaatcgt acgggcagca gcaaacggga agtagcgatt
    35461 atcggcggtg gtattgccag cgcattgttg tcgctggcgc tattgcgtcg cggctggcaa
    35521 gtaacgcttt attgcgcgga tgaagccccc gcgctgggtg cttccggcaa tcgccagggg
    35581 gcgttgtatc cgttattaag caaacacgat gaggcgctaa accgcttttt ctctaatggg
    35641 tttacttttg ctcgtcgact ttatgattca ttacccgtta aatttgatca tgactggtgc
    35701 ggcgtcacgc agttaggctg ggatgagaaa agccagcata aaatcgcaca gatgttgtca
    35761 atggatttac ccgaagaact ggctgtagcc gttgaggcaa atgcggttga acaaattacg
    35821 ggcgttacga caaattgcag cggtattact tatccgcaag gtggctggct gtgtccggca
    35881 gaactgaccc gtaatgtgct ggaactggcg caacagcagg gtttgcagat ttattatcaa
    35941 tatcagttac aggatttatc ccgtaaggat gactgttggt tgttgacgtt tgcaggagat
    36001 cagcaagcga cacacagtgt agtggtactg gcgaacgggc atcaaatcag ccgtttcagc
    36061 caaacatcgt ctctgccagt gtattcggtt gccggtcagg tcagccatat tccgacgaca
    36121 cccgaactgg caaaactgaa acaggtgctg tgctatgacg gttatctcac gccgcaaaat
    36181 ccggcgaatc agcaccattg tattggtgcc agttatcatc gcggcagcga agagacggcg
    36241 tacagtgaag acaatcagca gcagaatcgc cagcgattga ttgattgttt cccacacgca
    36301 caatgggcga agacggttga tgtcagtaaa aaagaggcgc gctgcggtgt gcgttgtgcc
    36361 acccgcgatc atctgccaat ggtaggtaat gttcccgatt atgaggcaac actcgtggaa
    36421 tatgcgtcgc tggcggagca gaaagataag gcggtaagcg cgccggtttt tgacgatctc
    36481 tttatgtttg ctgctttagg ttcgcgcggt ttgtgttctg ccccgctgtg tgccgagatt
    36541 ctggcggcgc agatgagcga cgaaccgatt ccgatggatg ccagtacgct ggcggcgtta
    36601 aatccgaatc ggttatgggt gcggaaattg ttgaaaggta aagcggttaa ggccgggtaa
    36661 tctgctctcg cagtgtttgc cggatgcggc gtgaacgcct tatccgacct acgtgtgacg
    36721 tgtaggcctg ataagacgcg ataagcgtcg catcaggcat ggtgctccag acgccgcaac
    36781 ttactgctgt gacgcctgct gaaacaggtt ttcccacata tcggtcacca gcgcctggtc
    36841 acgcggcgac aattcaccag caccaatggc tttttccaga ctctggctaa cggtcgtatg
    36901 caccgcctga gcggagtggt catcaccact ttccagttct gcgatggcta atgtaaggtg
    36961 gccacgcaaa tacccactgg caaacaactc atcatcactg gcatggtcaa ccataccgtc
    37021 gattaattcc agaatgcgtg attcaaactc cgcgatcatc ttctttcctc attgtaaaac
    37081 gtggcgctgg cttcagttga gcgcttccgg ccacgggaac tgttccgccg taagttccgg
    37141 cgtgtgataa taattttgta gtgctttaat gaaacgtgcc gggcgttccg gaatgccatt
    37201 tgttagataa tccatcacct gcgcatgtac tcgccgttga aacaccacgc ggtccggttc
    37261 gaagtcgcct tccagattgt cgcaactgac gttaaacggg tatcccgccg ccacgcagaa
    37321 caaccagtcg agcgcctgcg gcttcacttc aacatcttca aactggcttt gcgtttgggc
    37381 atcgcgaccg tccgggcaat accagtagcc gaaatcaacc tgctcgcggc gcgctttccc
    37441 ggcaatacac cagtgcgaaa tctcatgaat tgcgctggca taaaagccgt gggcaaagac
    37501 gattcggtta tacggtactt ccgcatcagc gggaagatag atcggttcgt cgtcgccttt
    37561 aatcagacgg gtattaaaat catcggcaaa gcagctatta aaaatttcaa tcaactgctc
    37621 atagtggtgt gtactgttca ttagttcatc cccaaccagt ggaggatctc ctgtccgtgg
    37681 ctatcataaa gtaatttggc actcatcacc gccgagacga taacgatcat cgggcggatc
    37741 agtttttgtc ctttgctcaa caccagtctt gaacccatgc gcgcgccaag gaactgcccc
    37801 accagcatca caaagcccgt cgcccaaatc actttgccgc cgagaataaa cagcagtaaa
    37861 ccgccgatgt ttgacgttgc gttgagtaat ttggcgtgag ccgtggcttt ggcgaggttg
    37921 aatccgcaca gcgtaacaaa ggccagcgcg taaaacgatc cggctgccgg accaaagaat
    37981 ccgtcataaa aaccgacgca gccaccggca attaacgcga agggcaaacc gtacatccgg
    38041 cgctggcgat cttcttcacc cagctttggc atcagcaaga aatagaggcc gatacaaatc
    38101 accagaatgg gcaaaatctg ccgtaagaca tcagcctgaa cgtactgtac caacagtgcg
    38161 ccgctcattg agccgacaaa ggtcatggca atattaagtt tctgatcgct taaactaacc
    38221 actttgcggc gaataaagta gatagtggcg gaaatagagc cgccgcaggc ttgcagttta
    38281 ttggttgcca gagcattagc gggagacatc cccgctgcca tcaatgccgg aatggtgagt
    38341 aacccaccgc caccggcaat cgagtcgata aatcccgcca gcatggcgac aaaaaagaga
    38401 actcccagca acagcgggga aaccataaac aggctattaa acgtttccat tagatcacgt
    38461 gctcatccag tagcgcctgg caggaaggcg gcaacggagg cggtgtcttc ttctcaggct
    38521 ttgttgttcc cggttttgga ggttcaaacc agctttgcag ttctgccccg caaccatcgc
    38581 ctggtggcgg taaaggttga tcttcacact ccagactatc ggcaggacaa cgtaatcgta
    38641 catgcatatg cgcacgatgc tggaaccagg gtcgcacttt gcgcaaccag tcgcgatcgg
    38701 tgcccgcatc aaggcaaagt tgttgtttaa tcgccggatt aacaaaaatg cgcgtgacgt
    38761 ctttgtcctg ggcggcgagt ttgatcaagc tgaaaatttc tggcttccac agcgtggaga
    38821 caacgtgttt accgtcgcgg gaaactaagt ccagtgcttg cgggcgcaag agctgcgcgg
    38881 aggtccggcg tgttttcggc agttgcagaa agatatcgac atccagtccg gtctggtggc
    38941 tggcgtgacc gccgttgaaa cgcccaccag caggcattcc catatcgcca atcaacaccg
    39001 tacccatgcc cagattgctc acctgactac tcaggcgctg gataaacatc accaggtccg
    39061 ggtgaccgaa atagcgacgc tgatcggtac gcatgacctg ataatgttcg gactgtatcg
    39121 gcaacgtgtc agcgccgacg atacaaccat tggaaaaact gcctatcgat tgtgcgctac
    39181 ccggcacagg ttgggttatt ttttgccacg gcgtcgctgc caggctgacg ctactggcaa
    39241 gcagggccag cagcgcaatc gcggttttgt tcatttttta ccagcgtgga atatcagtct
    39301 tcacatcggc attttgcgcc cgctgccgta acaggtgatc cattaaaacg atcgccagca
    39361 tcgcttctgc tatcggcacc gcgcggatcc cgacacaggg atcgtgacgg cctttggtga
    39421 tcatctcaac ttcttcgcca aagcggttaa tggtgcgccc cggcacggta atactggagg
    39481 tcggtttcag tgccatatga gcaatgattt gctgcccgct gctaattccg ccgagaatgc
    39541 cgcccgcatg gttactctgg aaaccgtctt tggtgatttc gtcgcggttc tggctgccac
    39601 gcagcgccac cacgtcaaaa ccatcaccaa tttccacgcc tttcaccgcg ttaatgctca
    39661 ttagcgcatg ggcgatgtcg gcatccaggc gatcaaagac cggctcacca agcccggcag
    39721 gaacgccact ggcaacaacg gtgactttcg cgccaataga gtcgccctct tttttcagcg
    39781 cgcgcatcag ttcatctaac gcgtcgattt tgtccgggtc cgggcagaag aatggatttt
    39841 gctcgacctg cgaccagtct ttgatttcca gcggaatgtc gcccatctgg gtcaggcagc
    39901 cgcgaatttc gatgccaaat ttctcagcga gatatttttt ggcaatcgcc cctgctgcaa
    39961 cgcgcatggc ggtttcacgg gcggaagaac gtccaccgcc gcgataatcg cgcagaccgt
    40021 atttttgttc gtaggtgtaa tcggcatggc caggacggaa aacgtcctta atcgcactat
    40081 aatcctgaga acgctgatcg gtattttcga tcaacaaacc aatgctggtg ccggtagtaa
    40141 caccttcaaa aacaccggag agaattttga cctgatctgg ctcacgacgc tgggtggtat
    40201 agcgcgatgt cccagggcga cgacggtcga ggtcatgttg caggtccgct tccgtcagcg
    40261 gaatgcctgg cggaacacca tcgacgatgc agccgagcgc cagcccgtgc gattcgccga
    40321 aggtggttac gcgaaagagt tgtccaattg tgtttccagc catcacggct ccgttattgt
    40381 tgtgtttgcg tgtttactta atctttataa atcgcgaaat gttcacgggc cgccagcagc
    40441 tgcgctttgg tgagcataaa cacaccatcg ccgccgttat caaactccag ccaggtgaac
    40501 ggaacatcgg gatattgttc cataagatgt accatgctgt tgccgacttc acaaatcagt
    40561 acgccatcat cagcaagata atctgccgcg tttccgagaa tgcgacgcgt cagtttcagg
    40621 ccgtcggtgc cagatgccag gcccagttcc ggctcgtggc ggtattcatt tggcaggtcg
    40681 gacatatctt ccgcatcgac atacggcggg ttagtgacaa tcaggtcgta ctgcactttc
    40741 ggcaaatcac ggaacagatc ggaacgaatc ggtatgacgt tgtggatcag accgtgttct
    40801 tcgatgttct gttcggcaac cgccagcgcg tcgggagaga tatctaccgc atcgacttcc
    40861 gcttccggga aggcataagc acaggcaatg gcgatgcagc cgctaccggt acacatatct
    40921 aaaatatgct gcggttgctt gctgataagt ccggcaaatt tattgttgat caattcacca
    40981 atcggcgagc gcggcaccag cacgcgttca tcgacgtaaa attcatggcc gcagaaccac
    41041 gctttgttgg tcaggtaagc taccggaatg cgttcgttga cgcggcggat cacgcgctca
    41101 acaatacgat gtttttcgct ggaggtcaga cgcgcggtgc gcatatcttc cggaatatcc
    41161 agcggcaggt agagcgacgg caataccagc tgtacggctt catcccacgg gttatcggtg
    41221 ccgtgaccgt accagatatt tgccgcgctg aagcggctca ccgaccagcg caacatgtcc
    41281 tgaatggttt gcagctcatt tactgcttca tcaacgaaaa ttttatccac gtattcctcc
    41341 agggcatgat cgcaataatt tcggcggcta gtttgccatg aagatgacga taaatcagca
    41401 ttcacgcgcg gtgagtgagg aaaaatacgt ttaaaacgat cgattgcgct acgagtcggg
    41461 taaactgtag gaaaattaga aatagagaca gataaatgaa aaagaaaaca acacttagcg
    41521 aggaggacca ggctctgttt cgccagttga tggcggggac tcgcaagatt aagcaggaca
    41581 ccattgtcca tcggccacag cgtaaaaaag tcagcgaagt gcccgtcaaa cggttgattc
    41641 aggagcaggt tgacgccagt cactatttct ctgacgaatt tcaaccgtta ttaaatgctg
    41701 atggcccggt gaagtatgta cgccctggtg tagaccattt tgaggcgaaa aagctgcgcc
    41761 gcggcgatta ttcgccggaa ttgtttttgg atttacacgg tctgacgcag ctgcaggcca
    41821 agcaggaact gggcgcgttg atcgccgcct gtcgccgcga acatgtgttt tgcgcctgcg
    41881 tgatgcatgg tcacgggaaa catatattga agcaacaaac accgctgtgg ctggcacaac
    41941 atccgcatgt catggcgttt caccaggcac caaaagagta tggtggtgat gccgcattgc
    42001 tggtgttgat tgaagtggaa gagtggctac cgccggagtt gccatgaata ttagggggcg
    42061 gatgcgtttt gccatttcgc cctgaatgct ggtatcgaat gccggatgcg gcgtaaacgc
    42121 ctgatccggc ttatcaaaat gtgcaaattc aatacgttgt gattcagttg taggcatgat
    42181 aagacgcgtc agcgtcgcat caggcatcaa cgcacaacta cagttcattc agtacatact
    42241 ggaaacggca tgtagaatca attggctcac ggctactggt attcacctgt accacaatgt
    42301 cgtgtagctt tctatccgtc aatggttgac gtgagggggt gactgtcacc tttgcattat
    42361 cacaaccata cgaggtaaag gttaataagt cagaaacagc acgacaatat gcccctctgg
    42421 aatcaaaaat actgccatcg cagagtttgc cgctttccat caggtaggtc gaaagcgtag
    42481 tcgtgattgt cgaaccatcg gattgcaatc gcagcgcttt attatggcta acataattat
    42541 cactccagga tatatcaaac gaagcgacgg gaattgtcgg gcaaatagaa tcatgaactg
    42601 ttgggcttgt atacgtacca aatccaaatg cctcagatac tgaataaaat gaggcaatcc
    42661 acggacaacc ataattatca gattctacag tgacgttaaa agtcatatat gcacagacaa
    42721 agacacgagt attgatacca gggcaatttg aggtgtccgt acgtctataa tcagtcaaaa
    42781 ccagtgtcgg tgccgggcca gatccgctcc aggatattaa cttgagggtg tcgccttttc
    42841 ttgcattgga gttgtattgt ccgtagctta cttctcccgg cgtcactacc gtgacataga
    42901 aatataaact gccggaagca gtaacgtttt tcatcgcata ataaatttta gtactggaaa
    42961 agcttgcact catgagttta tcaccggcat agcactttgg tgtttctaaa ctgagagcaa
    43021 gtaatatcag acttgtcaaa aatatcgtcc attttttcat tttaaaaata ctcaaaagtt
    43081 accgtcattg tggcggtgaa atcacccgat gtaacatttc gtccatttac ggtctgtaac
    43141 caggcattaa aattgacact gttatttccc tgatttaatg gaaatgaggc acctgtggta
    43201 ctgttaatag gtacagccgc ccctccggca gtttcaatgc caatcccaac acccgttgca
    43261 gaagatgacg aatcaatcga cagaaatcct ggtaagtcgg tatcttctgt tcccatcaag
    43321 gtcaccttga cattaaacgc cgtggtgctt ttgcaatctt tcaactgaaa aactaacggt
    43381 acgcgatctg actgaccacg ttgcattaaa tcgctggcag caattgtggg gaaatgaatt
    43441 tctgcaagta agttgccatt gattacagga gtacaggatt taccaagcaa attaccggta
    43501 aaatgcaggt tatcggcagc tggggccgtt ccgctgaaca atatcagacc actgataagc
    43561 gcccacgcac ggttattctt cattggtaat ccacctgtaa cgtagcccat gcttcaaagt
    43621 ctgactcggg taagtcgaca ccgctctttt ttacgggtac tgccgttagt accggaaggg
    43681 atgtttcatt caccactaag ggcgtatgca gcttaaaatc gctcccggct tgtttgaggt
    43741 gtattcctaa tccattgaga tccgtggcta cggccgaatc gtcaaagtca ctctgcgtac
    43801 cactccaggt caacgtcatt tccatttgag ggtcacggac agcggtatcg caggtaatgg
    43861 tatacggcac gacctgttcg taattgctac cgttaatgtc atcaatattc acgtcccgaa
    43921 acgtcaactc tatattttgc ccattactaa tagagcaatt gggtggctgc actaaataac
    43981 catgaaaaga gacattcgag agcgccataa agctaaagct ccacaacatt aagagagaga
    44041 tccgtttcat tgataatcca ccacaattgt ggcactggcg ctaaattccg ccgccgttag
    44101 cgatacaccg ctctgcacga caggaaccgc ttccagagcc agtgcgccag atgaaaagtt
    44161 aaacggtaac catgcgccag gcgttaaatc caaaatggag tgatcgctta ctttctgaat
    44221 acggatacca aaaccggcaa tacccgtatc gattaccgtt tctccgttaa ttacggttgt
    44281 ggttgcctgc atatttaagc gcaggtcgtc cattgccaga ttgttgcaca caagcgagta
    44341 gtcaatgggg cgtttgtaac ttaccccatc gatcttggaa atgaagacat taccaaactc
    44401 cacgctcgca cccgttaccg tacagggcgg cggggcatca acgagcacac gcaagaccat
    44461 cgcaaccgat ttactccccg ccattacagg cgtgctcaca atgctacaca gcaataacca
    44521 cgcccaggcc gaataagctc ctttcatgct attgctccta tccctggcct ttgctgtttt
    44581 gctcttcgtc cacctgacag gtatttcccg tacagcggaa gaacagcggc aaacgcgcac
    44641 cataatcatt cacataggtc agcaccggga cgctatccat tttgactttt aacggcacag
    44701 aggtttttgg cgatatcacc atgggggaaa atcccgctgc cggattgcca tttttctgcg
    44761 cactggcgtt actgataacc acgaagtacg gcgtcgggtt attgacggta aactcctgac
    44821 cactgagggt taaggtcacc ttatgctgcc acgggttttt catgctgacg ttttccagcg
    44881 cctttggtcg ccagaacaac ttaatgcgcg tctgcagcgc tatctgtagc gtgttaggtt
    44941 tgttcgattt cggcgggatc tcacgcacgt tgaaatagaa cacgctctcg cggtcggcag
    45001 gcagcttgtt gatatccggc atgccctgta ccttcacctg accgttcatc atggaatcga
    45061 tgcgctgcac tggcggcagg accgtcagtg gcgagctaat cctgttaccc tgtccatctt
    45121 ccatccagct ttgtgccaga tacggcattt tcgggtcgtt attgcgcagc gtgacgctga
    45181 ttgatttatc actttcgtta aaaatcaggc gtgtacggtc cggcgtgaca gaggcctgta
    45241 ctgacaggct ggcggcagtt atcagtaacg ccagcgtcgt tgcggccgtt ctggcagagc
    45301 aaaatagatt cgtcatggat cattccttaa tcacgttatt aattggctga taccgatact
    45361 ggcgcttccg ttggcatcac ctgctgggtc tgctcctgga tcaccggctt aatttcatga
    45421 ggcatcgaag gagatatcgc ccctgtttgc tggcatggta ataacaggcc attgaacaga
    45481 tcggctggca gcgggtcagg cagatgaata tcgcagtggg ccacaccgcc ccatgaaacg
    45541 atcatatgct cgccaggttt tacgcccgcg aggtagacgt tgccgtcatc gtcaaccaga
    45601 ccgacgttct gcgcgctgtc gtttttcact tccgcgccaa acggcggata actgccatct
    45661 tgcagacgca gcaccgccat cgctttttgc ccgctgatca ccgagaactt acggtagcca
    45721 atggcccctt cggtaagcgt ggcctgcacc acggactggg tggcttcggc gttttccggc
    45781 aggttgttta ggtcgatata cgcctggttg cggtagtagt tgttgacgtc tgccaccact
    45841 gccttaccga acatattggt gtaaaccgcc gcgccatttc cttccaccgg aacaccagcc
    45901 acaccgtcgg catcaatcag cagacgcgta ccgcccatat tctgggtacg gtggagcgcg
    45961 ccaccttgtg cggtgagcgt cgcgccgccc tgtaaggaaa tacccgccga ggtgtactgc
    46021 ccttcatggt agttagcgct gagatcgacc tgcgccagcg atccgtcgtg gctgtaataa
    46081 ccgtcaacgc tggagtgatt gtcgctggtg cctacgttca actggtaatg ggttgcgtca
    46141 tcgacacggc tgaaataccc cacctggctg ctgtccgaac cgctgccgta gttgccgtta
    46201 tagctgatgg tgctgctgtc accccacggc atactgagcg atatgtacac acctttatcg
    46261 gcctggttgt catactcgta gcggtatccg gtcatggaaa tgctcatgtt gcggatactg
    46321 cccaggttga agtagtgcga gagcatgacg ttgtagttgg tctgttcgtc gcgatcccag
    46381 taggtatggc gggtgtagtt gagataaaca gacacaccgg catccctgaa gttctggtta
    46441 taggtggcgg tgtacatctc tttgtcgttg ccggtgcgca ccatttcgct gtcgctggca
    46501 tcgagatact cgctcatggt catgaagttc tcttccgaga agcggtatcc ggcaaaagtg
    46561 acgcggctgt tcagttcatc gaagtctttg gaatagctca ggcgaaacga gttgccgtcc
    46621 agtgaacctt tcccgtaggc ggtctcttta tccagacggg tatgcgagtg ggtgatatca
    46681 aaggccaccg caccaaacac agacaggtcg cgaccgacgc caagcgccgc cgactgatag
    46741 tgttcatctg ccagcgcccc gccgtatagc gaccagccgt tggcaatccc ccaggaagct
    46801 tcgccgccgg agaagaaacc gccttccacg tggtgccccc actcctgcgg gcggcccatc
    46861 atcagtttat agcgcacctg gccggggcga gtcaggaacg gcatcgaggc ggtgttgatg
    46921 tcatattcct gcacctgacc gttctgttct tcaatgcgga tatgcagcgt accggagacc
    46981 gaatcgccaa gatcctggat gcggaacggc cccgccggga cctgggtttc gtagatgacg
    47041 cggcccaact ggctgacggt cactttcgcg gtggtgtgcg ccacgccgga gatatccggc
    47101 gcatagccgc gcagattcgg cggcaacatt tgatcgtcgg tgctgatact gccacccacg
    47161 tagttaaagc cgtcgaaaat atcagaattc aggtagtctt cgccaaggcc aagtttggct
    47221 tttagcgacg gtaaggctcg ccaggcgtag tagcggctcc actcccagtt tttttgcgtg
    47281 tcatcaccgt tgataacatc gtcatcattg cttttactat gcaaataatc agtctgccag
    47341 tcggcacgaa gacgccatgc gccgaggttc accccaaccg tcccgttacc gctaatttca
    47401 ttgctgtcat ccccgccatt ttcttcatgt cgtgtctggg cggtaatact gtagtcagca
    47461 attaaaccag atataccgtc atcccagcgt gaaggcggat cccagttgat gtcggtatat
    47521 tcaaggtaag cctggggtaa tgaaatgact aacgctgact gactcaggtc agctttaata
    47581 tcaatgcctt ccagttgacc gggtttcagg catttgccgt cgtggatcca ttgcaggttt
    47641 tttgccacat cctcttttaa gccaaactgc gcgaccagtt caggcgtcag gcaggcatag
    47701 gttttactgg catcgttctc agaggcgtac cagtaaatat cgtattcttc cgtcagcggc
    47761 tgtttattta gttgaacctg taaattgtat ttcccaggtt cgacataacc ctggctggaa
    47821 aatcgcttca gatcgatttt tgtgtcgcct tttaactcca gaaaacggga atcaaactga
    47881 atttcatttt cagcccaggc attgacataa ctcccggaca ttgccaatgc aacgcagcag
    47941 ggaagtaccc gcagtcggaa aagagaatgg tcaggcatac acgtatccat ccgtaattat
    48001 tataatttgc gcgaattcca tttaaaaacc ccatagcccg aaagctatgg gggtatcact
    48061 tagagataag taatctggaa ggtcgtgttg gcttcaaaat tacctaaatc tggcgcatca
    48121 gcagcgccca ccagccaggc tttaaagtcc agcgtctgct tcgctttgcc tttgttggtc
    48181 gccgtatcgt ttacgatttt ctgttcgata cccgcgccgc ttttataaga ggttccctga
    48241 gcgtcaccaa tggccaggct gacattgtta aatgccgcac cagtatcggt attgtaaatg
    48301 gtgtagtaat tgccgctgtt ggtagaagac gcgttaccgg tgaaggtagt ggtcatcgtt
    48361 gtctgggtgt caaacacaca gtcttgcaga tggatctgga aagctttcgg tgtagaagtc
    48421 ccgttatttt tcaggatccc tgaaccaatg tcacccatat caacttccag tttgtgatca
    48481 tcaggaacga tagagcacgg ggaatcttca atggtgccgt agaaacgtgc tgtaccattg
    48541 ttgccagcgg cgattgttga agtagaagcg aacgcaccca ttaccattgt tgcagcaata
    48601 gctgttttta caaacttact cattatttgt ccttaaacca taaataatta aaaatataaa
    48661 cattgcaata cattgattca gtcaatagca attatttata cccagccatt gaataagcta
    48721 tccgatctaa ctaacaagaa aataggtcgc ataagaagat attctttggt gtgggcggat
    48781 ggtgatattt tgtctttatt gtttcaaatt atattt
//
LOCUS       DAZKIP010000020        48080 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZKIP010000020 DAZKIP010000000
VERSION     DAZKIP010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48080
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.020"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            230..1546
                     /gene="fucP"
                     /locus_tag="ACHOUK_001421"
     CDS             230..1546
                     /gene="fucP"
                     /locus_tag="ACHOUK_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFP9896897.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGSHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            1579..3354
                     /gene="fucI"
                     /locus_tag="ACHOUK_001422"
     CDS             1579..3354
                     /gene="fucI"
                     /locus_tag="ACHOUK_001422"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFP9896898.1"
                     /translation="MNKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            3463..4881
                     /gene="fucK"
                     /locus_tag="ACHOUK_001423"
     CDS             3463..4881
                     /gene="fucK"
                     /locus_tag="ACHOUK_001423"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFP9896899.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIERLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPLKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            4883..5305
                     /gene="fucU"
                     /locus_tag="ACHOUK_001424"
     CDS             4883..5305
                     /gene="fucU"
                     /locus_tag="ACHOUK_001424"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFP9896900.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            5363..6094
                     /gene="fucR"
                     /locus_tag="ACHOUK_001425"
     CDS             5363..6094
                     /gene="fucR"
                     /locus_tag="ACHOUK_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFP9896901.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTG"
     gene            complement(6138..7238)
                     /gene="rlmM"
                     /locus_tag="ACHOUK_001426"
     CDS             complement(6138..7238)
                     /gene="rlmM"
                     /locus_tag="ACHOUK_001426"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFP9896902.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7231..7626)
                     /gene="ygdD"
                     /locus_tag="ACHOUK_001427"
     CDS             complement(7231..7626)
                     /gene="ygdD"
                     /locus_tag="ACHOUK_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFP9896903.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(7645..8562)
                     /gene="gcvA"
                     /locus_tag="ACHOUK_001428"
     CDS             complement(7645..8562)
                     /gene="gcvA"
                     /locus_tag="ACHOUK_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFP9896904.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(8913..9140)
                     /gene="ygdI"
                     /locus_tag="ACHOUK_001429"
     CDS             complement(8913..9140)
                     /gene="ygdI"
                     /locus_tag="ACHOUK_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFP9896905.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            9332..10537
                     /gene="csdA"
                     /locus_tag="ACHOUK_001430"
     CDS             9332..10537
                     /gene="csdA"
                     /locus_tag="ACHOUK_001430"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFP9896906.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGIVVIVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAENWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            10537..10980
                     /gene="csdE"
                     /locus_tag="ACHOUK_001431"
     CDS             10537..10980
                     /gene="csdE"
                     /locus_tag="ACHOUK_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFP9896907.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(11031..11837)
                     /gene="tcdA"
                     /locus_tag="ACHOUK_001432"
     CDS             complement(11031..11837)
                     /gene="tcdA"
                     /locus_tag="ACHOUK_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFP9896908.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINSECCV
                     TVVDDFVTPDNVAQHMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(12076..13173)
                     /gene="mltA"
                     /locus_tag="ACHOUK_001433"
     CDS             complement(12076..13173)
                     /gene="mltA"
                     /locus_tag="ACHOUK_001433"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFP9896909.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            13382..13458
                     /locus_tag="ACHOUK_001434"
     tRNA            13382..13458
                     /locus_tag="ACHOUK_001434"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13416..13418,aa:Met,seq:cat)
     gene            complement(13532..14785)
                     /gene="amiC"
                     /locus_tag="ACHOUK_001435"
     CDS             complement(13532..14785)
                     /gene="amiC"
                     /locus_tag="ACHOUK_001435"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFP9896910.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            15017..16348
                     /gene="argA"
                     /locus_tag="ACHOUK_001436"
     CDS             15017..16348
                     /gene="argA"
                     /locus_tag="ACHOUK_001436"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFP9896911.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(16410..18236)
                     /gene="recD"
                     /locus_tag="ACHOUK_001437"
     CDS             complement(16410..18236)
                     /gene="recD"
                     /locus_tag="ACHOUK_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFP9896912.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEESHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPTGDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRNPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(18236..21778)
                     /gene="recB"
                     /locus_tag="ACHOUK_001438"
     CDS             complement(18236..21778)
                     /gene="recB"
                     /locus_tag="ACHOUK_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFP9896913.1"
                     /translation="MSDVAETLDPLRLPFQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAKWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     RNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVSAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMMRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLNFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQARGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIDLMDEMFAGMTLEEA"
     gene            complement(21771..24659)
                     /gene="ptrA"
                     /locus_tag="ACHOUK_001439"
     CDS             complement(21771..24659)
                     /gene="ptrA"
                     /locus_tag="ACHOUK_001439"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFP9896914.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEALKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(24835..28203)
                     /gene="recC"
                     /locus_tag="ACHOUK_001440"
     CDS             complement(24835..28203)
                     /gene="recC"
                     /locus_tag="ACHOUK_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFP9896915.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNALFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVSGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(28216..28539)
                     /gene="ppdC"
                     /locus_tag="ACHOUK_001441"
     CDS             complement(28216..28539)
                     /gene="ppdC"
                     /locus_tag="ACHOUK_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFP9896916.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLTSR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGRAGEMTRLH
                     CPNRQ"
     gene            complement(28524..28931)
                     /gene="ygdB"
                     /locus_tag="ACHOUK_001442"
     CDS             complement(28524..28931)
                     /gene="ygdB"
                     /locus_tag="ACHOUK_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFP9896917.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCLLYAATGARVCLRLLEDNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(28928..29491)
                     /gene="ppdB"
                     /locus_tag="ACHOUK_001443"
     CDS             complement(28928..29491)
                     /gene="ppdB"
                     /locus_tag="ACHOUK_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFP9896918.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGICTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEQVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(29482..29952)
                     /gene="ppdA"
                     /locus_tag="ACHOUK_001444"
     CDS             complement(29482..29952)
                     /gene="ppdA"
                     /locus_tag="ACHOUK_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFP9896919.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISFIREGTLWCLVSSVAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIGFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(30136..30930)
                     /gene="thyA"
                     /locus_tag="ACHOUK_001445"
     CDS             complement(30136..30930)
                     /gene="thyA"
                     /locus_tag="ACHOUK_001445"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFP9896920.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(30937..31812)
                     /gene="lgt"
                     /locus_tag="ACHOUK_001446"
     CDS             complement(30937..31812)
                     /gene="lgt"
                     /locus_tag="ACHOUK_001446"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFP9896921.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(31963..34209)
                     /gene="ptsP"
                     /locus_tag="ACHOUK_001447"
     CDS             complement(31963..34209)
                     /gene="ptsP"
                     /locus_tag="ACHOUK_001447"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFP9896922.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(34222..34752)
                     /gene="rppH"
                     /locus_tag="ACHOUK_001448"
     CDS             complement(34222..34752)
                     /gene="rppH"
                     /locus_tag="ACHOUK_001448"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFP9896923.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(35105..35251)
                     /gene="ygdT"
                     /locus_tag="ACHOUK_001449"
     CDS             complement(35105..35251)
                     /gene="ygdT"
                     /locus_tag="ACHOUK_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFP9896924.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            35437..36126
                     /gene="mutH"
                     /locus_tag="ACHOUK_001450"
     CDS             35437..36126
                     /gene="mutH"
                     /locus_tag="ACHOUK_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFP9896925.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            36195..36908
                     /gene="ygdQ"
                     /locus_tag="ACHOUK_001451"
     CDS             36195..36908
                     /gene="ygdQ"
                     /locus_tag="ACHOUK_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFP9896926.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            37046..37264
                     /gene="ygdR"
                     /locus_tag="ACHOUK_001452"
     CDS             37046..37264
                     /gene="ygdR"
                     /locus_tag="ACHOUK_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFP9896927.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            37372..38412
                     /gene="tas"
                     /locus_tag="ACHOUK_001453"
     CDS             37372..38412
                     /gene="tas"
                     /locus_tag="ACHOUK_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFP9896928.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLAPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(38444..39637)
                     /gene="lplT"
                     /locus_tag="ACHOUK_001454"
     CDS             complement(38444..39637)
                     /gene="lplT"
                     /locus_tag="ACHOUK_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFP9896929.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHEQLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(39630..41789)
                     /gene="aas"
                     /locus_tag="ACHOUK_001455"
     CDS             complement(39630..41789)
                     /gene="aas"
                     /locus_tag="ACHOUK_001455"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFP9896930.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDPQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEVEQHDE"
     gene            42375..43406
                     /gene="galR"
                     /locus_tag="ACHOUK_001456"
     CDS             42375..43406
                     /gene="galR"
                     /locus_tag="ACHOUK_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFP9896931.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLLKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(43413..44675)
                     /gene="lysA"
                     /locus_tag="ACHOUK_001457"
     CDS             complement(43413..44675)
                     /gene="lysA"
                     /locus_tag="ACHOUK_001457"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFP9896932.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMFDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVEPRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            44797..45732
                     /gene="lysR"
                     /locus_tag="ACHOUK_001458"
     CDS             44797..45732
                     /gene="lysR"
                     /locus_tag="ACHOUK_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFP9896933.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLVRYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDELCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(45719..46411)
                     /gene="ygeA"
                     /locus_tag="ACHOUK_001459"
     CDS             complement(45719..46411)
                     /gene="ygeA"
                     /locus_tag="ACHOUK_001459"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFP9896934.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(46540..47958)
                     /gene="araE"
                     /locus_tag="ACHOUK_001460"
     CDS             complement(46540..47958)
                     /gene="araE"
                     /locus_tag="ACHOUK_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFP9896935.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT        11809 a        13070 c        11891 g        11310 t
ORIGIN      
        1 acgggcatga aatttcgatt attaaagtga tggtagtcac ataaagtcac cttccagcta
       61 ataagtgtga ccgccgtcat attacagagc gttttttatt tgaaaatgaa tccatgagtt
      121 catttcagac aggcaaatat tcactgatat gaagcccgaa ctcgctagtt ttgcactttt
      181 gaaaacataa ccgattacgt gcttaagctt ctgaacctaa gaggatgcta tgggaaacac
      241 atcaatacaa acgcagagtt accgtgcggt agataaagat gcagggcaaa gcagaagtta
      301 cattattcca ttcgcgctgc tgtgctcact gttttttctt tgggcggtag ccaataacct
      361 taacgacatt ttattacctc aattccagca ggcttttaca ctgacaaatt tccaggctgg
      421 cctgatccaa tcggcctttt actttggtta tttcattatc ccaattcctg ctgggatatt
      481 gatgaaaaaa ctcagttata aagcaggaat tattaccgga ttatttttat atgccttggg
      541 tgctgcatta ttctggcccg ccgcagaaat aatgaactac accttatttt tagttggcct
      601 atttattatt gcagccggat taggttgtct ggaaactgcc gcaaaccctt ttgttacggt
      661 attagggccg gaaagcagtg gtcacttccg cttaaatctt gcgcaaacat ttaactcatt
      721 tggcgcaatt atcgcggttg tctttgggca aagtcttatt ttgtctaacg tgccacatca
      781 atcgcaagac gttctcgata aaatgtctcc agagcaattg agtgcgtata aacacagcct
      841 ggtattatcg gtacagacac cttatatgat catcgtggct atcgtgttac tggtcgccct
      901 gctgatcatg ctgacgaaat tcccggcatt gcagagtgat aatcacagtg acgccaaaca
      961 aggatcgttc tccgcatcgc tttctcgcct ggcgcgtatt cgccactggc gctgggcagt
     1021 attagcgcaa ttctgctacg ttggcgcaca aacggcctgc tggagctatt tgattcgcta
     1081 cgctgtagaa gaaattccag gcatgactgc aggctttgcc gctaactatt taaccggaac
     1141 catggtgtgc ttctttattg gtcgtttcac cggtacctgg ctcatcagtc gcttcgcacc
     1201 acacaaagtc ctggccgcct acgcattaat cgctatggca ctgtgcctga tctcagcctt
     1261 cgctggcagt catgtgggct taatagccct gactttatgc agcgccttta tgtcgattca
     1321 gtacccaaca atcttctcgc tgggcattaa gaatctcggc caggacacca aatacggttc
     1381 gtccttcatc gttatgacca tcattggcgg cggtattgtc actccggtca tgggttttgt
     1441 cagtgacgcg gcgggcaaca tccccactgc tgaactgatc cccgcactct gcttcgcggt
     1501 catctttatc tttgcccgtt tccgttctca aacggcaact aactgaacat attttccgaa
     1561 taaagtgagg aatctgtaat gaacaaaatc agcttaccga aaattggtat ccgcccggtt
     1621 attgacggtc gtcgcatggg tgttcgtgag tcgcttgaag aacaaacaat gaatatggcg
     1681 aaagctacgg ccgcactgct gaccgagaaa ctgcgccatg cctgcggagc tgccgtcgag
     1741 tgtgtcattt ccgatacctg tatcgcgggt atggctgaag ccgctgcttg cgaagaaaaa
     1801 ttcagcagcc agaatgtagg cctcaccatt acggtaacgc cttgctggtg ctatggcagt
     1861 gaaaccatcg acatggatcc aacccgcccg aaagccattt ggggctttaa cggcactgaa
     1921 cgtcccggcg ctgtttacct ggcagcggct ctggcagctc acagccagaa aggcatcccg
     1981 gcattctcca tttacggtca tgacgttcag gatgccgatg acacatcgat tcctgccgat
     2041 gttgaagaaa aactgctgcg ctttgcccgc gccggtttgg ccgtcgccag catgaaaggt
     2101 aaaagctatc tgtcgctggg cggcgtttcg atgggtatcg ccggttctat tgttgatcac
     2161 aacttctttg aatcctggct gggaatgaaa gtccaggcgg tggatatgac cgaactgcgt
     2221 cgccgtatcg atcagaagat ttacgacgaa gccgaactgg aaatggcact ggcctgggct
     2281 gataaaaact tccgctatgg cgaagatgaa aataacaaac agtatcaacg caatgccgag
     2341 caaagccgcg cagttctgcg cgaaagttta ctgatggcga tgtgtatccg cgacatgatg
     2401 caaggcaaca gcaaactggc cgatattggt cgcgtggaag aatcacttgg ctacaacgcc
     2461 atcgctgcgg gcttccaggg gcaacgtcac tggaccgatc aatatcccaa tggtgacacc
     2521 gccgaagcga tcctcaacag ttcatttgac tggaatggcg tgcgcgaacc ctttgtcgtg
     2581 gcgaccgaaa acgacagtct taacggcgtg gcaatgctaa tgggtcacca gctcaccggc
     2641 accgctcagg tatttgccga tgtgcgtacc tactggtcac cagaagcaat tgagcgtgta
     2701 acggggcata aactggatgg actggcagaa cacggcatca tccatttgat caactccggt
     2761 tctgctgcgc tggacggttc ctgtaaacaa cgcgacagcg aaggtaaccc gacgatgaag
     2821 ccgcactggg aaatctctca gcaagaggct gacgcttgcc tcgccgctac cgaatggtgc
     2881 ccggcgatcc acgaatactt ccgtggcggc ggttactctt cccgcttcct taccgaaggc
     2941 ggcgtcccgt tcaccatgac tcgtgtcaac atcatcaaag gcctgggacc ggtactgcaa
     3001 atcgcggaag gctggagcgt ggaattgccg aaggatgtgc atgacatcct caacaaacgc
     3061 accaactcaa cctggccaac cacctggttt gcaccgcgcc tcaccggtaa agggccgttt
     3121 acggatgtgt actcggtaat ggcgaactgg ggcgctaacc atggggttct gaccatcggc
     3181 cacgttggcg cagactttat cactctcgcc tccatgctgc gtatcccggt atgtatgcac
     3241 aacgttgaag agaccaaagt gtatcgtcct tctgcctggg ctgcgcacgg catggatatt
     3301 gaaggccagg attaccgcgc ttgccagaac tacggtccgt tgtacaagcg ttaatacatt
     3361 actccctgat gtgatgcccg gtcgctccgg ctgccgggcc tgaacaagca agagtggtta
     3421 gccggataag caatgttatc cggctatatt gcaggagcga tcatgaaaca agaagtgatc
     3481 ctggtactcg actgtggcgc gaccaatgtc agggccatcg cggttaatcg gcagggaaaa
     3541 attgttgccc gcgcctcaac gcctaatgcc agcgatatcg cgatggaaaa caacacctgg
     3601 caccagtggt ctttagacgc cattttgcaa cgctttgctg attgctgtcg gcaaatcaat
     3661 agtgaactga ctgaatgcca catccgcggt atcgccgtca ccacctttgg tgtggatggc
     3721 gctctggtag ataagcaagg caatctgctc tatccgatca ttagctggaa atgtccgcga
     3781 acagcagcgg ttatggacaa tattgaacgg ttaatctccg cacagcggtt gcaggctatt
     3841 tctggcgtcg gagcctttag tttcaatacg ttatataagt tggtgtggtt gaaagaaaat
     3901 catccacaac tgctggaacg cgcgcacgcc tggctcttta tttcgtcgct gattaaccac
     3961 cgtttaaccg gcgaattcac tactgatatc acgatggccg gaaccagcca gatgctggat
     4021 atccagcaac gcgatttcag tccgcaaatt ttacaagcca ccggtattcc acgccgactc
     4081 ttccctcgtc tggtggaagc gggtgaacag attggtacgc tacagaacag cgccgcagca
     4141 atgctcggct tacccgttgg cataccggtg atttccgcag gtcacgatac ccagttcgcc
     4201 ctttttggcg ctggtgctga acaaaatgaa cccgtgctct cttccggtac atgggaaatt
     4261 ttaatggttc gcagcgccca ggttgatact tcgctgttaa gtcagtacgc cggttccacc
     4321 tgcgaactgg atagccaggc agggttgtat aacccaggta tgcaatggct ggcatccggt
     4381 gtgctggaat gggtgagaaa actgttctgg acggctgaaa caccctggca aatgttgatt
     4441 gaagaggctc gtctgatcgc gcctggcgcg gatggcgtga aaatgcagtg tgatttattg
     4501 tcgtgtcaga acgctggctg gcaaggagtg acgcttaata ccacgcgggg gcatttctat
     4561 cgcgcggcgc tggaagggtt aactgcgcaa ttacagcgca atctacagat gctggaaaaa
     4621 atcgggcact ttaaggcctc tgaattattg ttagtcggtg gaggaagtcg caacacattg
     4681 tggaatcaga ttaaagccaa tatgcttgat attccgttaa aagttctcga cgacgccgaa
     4741 acgaccgtcg caggagctgc gctgttcggt tggtatggcg taggggaatt taacagcccg
     4801 gaagaagccc gtgcacagat tcattatcag taccgttatt tctacccgca aactgaacct
     4861 gaatttatag aggaagtgtg aaatgctgaa aacaatttcg ccgttaattt ctcccgaact
     4921 attgaaagtg ctggcagaga tgggacatgg agatgaaatt attttttccg atgctcactt
     4981 tcccgcccat tcgatgggac cgcaggtgat ccgcgctgat ggcctgttgg tgagcgactt
     5041 gctccaggcg attatcccgt tatttgagct ggacagttat gcaccgccgc tggtgatgat
     5101 ggcagcggta gaaggtgaca ctctcgatcc tgaagtagaa cgacgttacc gtaatgcgct
     5161 ttcactacaa gccccgtgtc ctgacatcat ccgcatcaat cgttttgcgt tttatgaacg
     5221 ggcgcaaaaa gcctttgcga tcgttatcac aggcgaacga gcgaagtacg ggaatattct
     5281 tttaaaaaaa ggggtaacac cgtaatctca taccggtacg cccgcatgac gcgggcggtt
     5341 atcgaatgat ggggtgaaaa atatgaaagc ggcacgccag caagcgatag tcgacctgct
     5401 gctgaaccat accagcctga ccacggaagc tctctctgaa cagctaaagg tcagtaaaga
     5461 aaccattcgt cgcgatctca atgaattaca gacgcagggt aaaattctgc gcaatcatgg
     5521 acgcgctaaa tatatccacc gtcaaaatca agacagtggc gatccctttc acatcaggct
     5581 gaaaagccat tatgcgcata aagcagatat cgcgcgcgag gcgctcgcgt ggattgaaga
     5641 agggatggtg atagccttag acgccagttc aacttgctgg tatctggcac gccagttgcc
     5701 tgacatcaac attcaggtct tcaccaatag ccatccgatt tgccatgaac tcggtaaacg
     5761 cgaacgcatt caactgatca gttccggcgg cacacttgag cgcaaatatg gctgttacgt
     5821 caatccctcg ctgatttccc aacttaaatc gctggaaatc gatctgttta ttttttcttg
     5881 tgaagggatc gatagcagcg gcgcactgtg ggactccaat gctatcaacg ctgattacaa
     5941 atcaatgcta ttaaaacgcg ccgcgcaatc gttgttattg attgataaaa gtaaatttaa
     6001 tcgttcaggg gaagcccgca tcgggcatct ggatgaggta acgcacatta tttctgatga
     6061 acgccaggtt gcaacttctt tggtaacagg ctgacggaaa gggtagcagg ccggagacga
     6121 cgccccggcc ttgcctgtta tcgctcgtcg cgacgaccac ccaccgcagc ccagatacgg
     6181 cggacgtgca ccgtcacttc ttcgcgatcg tgatacaact gccgcgcctg aatctgagca
     6241 tttatgccat gttcatcaag ctgtgcctga atatacgcca gattgtgtga cacttcttcg
     6301 tagcgttttt tcatcggcag tttgaggttg aaaatggttt cacgacacca gccattaacc
     6361 agccactgcg ccatcaatgc cgcaactttc gccggttttt caaccatatc gcataccatc
     6421 caggagatat tgctgcgcgt cggacggaat ttgaaaccgt cttcccgcag ccacgtcacc
     6481 tgtccggtat ccatcagact ttgcgccatc gggccgttgt cgacggaata aacccacatg
     6541 ttgcgcttca ccagttggta ggtccagccg ccagggcaag cgcctaaatc caccgcccac
     6601 atcccgttcg ccaggcgttc atcccactca tccgcaggaa taaacacatg aaatgcctct
     6661 tccagtttga gcgtggaacg actcggcgca tctgccggaa atttcaggcg cggaatgccc
     6721 atatagaacg gcgaattatt gttgctgtat gagtaaccgg tatagcagca gcctggtgca
     6781 ataaagaata catgcacaac cggacgcttc ggcgtttcat agttcgccag caccccggca
     6841 tcgcgcaagg cagcgcgtag cggcacggtg aatttacggc agaatttcag caactctttg
     6901 ctttcgttgg tatcggcaac ttcaacacgc agttcaccgc ccttctctac tacgccctgc
     6961 aacatgccga caatgggggt aatacgatct tctggcggca aatgctgcaa gagttccccc
     7021 accacaaacc actggcgggc aaaaattaat gaactgaacg gcagctcacg gattaactta
     7081 tcgccatcat caggttgata acattcataa atgacataac ccgcattctc tttcacgcgg
     7141 gcaaaaccga aaatctcccg ctggccggct ttatcggtaa tttctgcggc gcactctttt
     7201 tcaaagcccg gacggcacag caatacaacc ttattcatga cttacgccct tgcgctttaa
     7261 acggatagca ccaactaaca ttaacgccca gcccgcaagg aagctcacgc cgccaaccgg
     7321 agtgacaaac gcccacaaac gcagatggga cagtgccagg caataaaggc tgccgctgaa
     7381 cagcaccgtg cctaacgcga ggaaaacgct actccagtaa aaccagatac tgatgcgacg
     7441 ctgcattgcc accgccagac ctaagatcgc cagcgtatga aacgcctggt attcgaggcc
     7501 ggtctggatc cagcccatct caacggcccc catggtttta cttaacacat gcgcgccaaa
     7561 agcgcccagt gccacaaaaa tgaagccgct aatggcggcg aaaatcagca taaaacggct
     7621 ggtcatggtc gtaccctacg taaattattg ttcataacga aagcggaatt tttcttgttc
     7681 agcagcggct ttcgccagga tccattggcg aaaggcggct attttaccca gttctgcctg
     7741 gctgtcatga caaaccagat aaaaagcgtt tttactgacc agaacatcat taaacgggca
     7801 aacaagacgt ccggcttcaa tttcagattg cgccatcacg ttatttgcca gcgccactcc
     7861 ctgcccgtga atagccgctt gcagcaccat ggcgctatgg ctaaaaattg gcccttgctg
     7921 aacgttgata tgatttaacc ccaattgacg agtatatgtc tgccagtcac ggcgtgacgc
     7981 atcatgtaat aacgtatgtt tagccagatc ttccggggtc ttcaagggtt tttcgccagt
     8041 cagcagtagc ggcgaacaca ccggcaataa atattcggcg tacagttttt ccacccgtag
     8101 ccccggccag ttgccccgac cataaaatat cgccacatca acatcatccg ccagcttatc
     8161 ttcctgacga tcaaccgcct ggattcgaac gtcaattccc ggataagctg aattaaagct
     8221 ggaaagtcgc ggaaccaacc aatgaatggc gaaactgggg agtaaactga ccgtcaacgc
     8281 ccccttggcg ctacgggcct ggagtttacg cgtcgcttcg gttaattgcg aaaatatctc
     8341 tttgatatcg aggaaatagc tttgcccttc ctcggtcagc aggagtgaac gattacggcg
     8401 gcggaacagt tttagcccca aaaaatcctc aagagacttg atttgatgac ttactgcggc
     8461 ctgggtcaca aaaagctctt ctgctgcgcg agtgaaactt aaatggcgtg ctgcggcatc
     8521 aaaaactcgt aaggcattta gcggtggtaa tcgtttagac atggctttta aactttgatg
     8581 ttaaatgaat ttaacaatta gatcacactg tgtaacctat tagttttttt aatctgagcc
     8641 attataaatt gtccgttgag cttctaccag caaataccta tagtggcggc acttcctgag
     8701 ccggaacgaa aagttttatc ggaatgcgtg ttctggtgaa cttttggctt acggttgtga
     8761 tgttgtgttg ttgtgtttgc aattggtctg cgattcagac catggtagca aagctacctt
     8821 ttttcacttc ctgtacattt accctgtctg tccatagtga ttaatgtagc accgcctaat
     8881 tgcggtgctt ttttttacct tgcgatcgcg aattactgat ccagttcgac catctctttc
     8941 acgtcggtac ggttgatctg ctgtttgttg ccattagcgt ctttatacga aatcataccg
     9001 gtatcgttat cagtctgtgg tttgccgtca gagacgatgg tacgtccgtc attggtgtgc
     9061 atcacatagt tcgaaccgga acaggcgctc agggcaaaag tcagcataca ggcagaaata
     9121 attgcggcag tctttttcat catcttctcc ttaaagcgat taataaatct taatagtatt
     9181 gattaacagg ctaaaattaa cgcctaacac tattcagcat atgttactga cgcggcttcg
     9241 ccaggatatc cagataattc tgatggttag cactctcctt gtatcaaagt gaattttgcg
     9301 tcacgatcgg tgcatcaagc cgaggagtac catgaacgtt tttaatcccg cgcagtttcg
     9361 cgcccagttt cccgctctac aggatgcggg cgtctatctc gacagcgccg cgaccgcgct
     9421 taaacctgaa gccgtggttg aagccacccg acagttttac agtctgagcg ccggaaacgt
     9481 ccatcgcagc cagtttgccg aagcccaacg cctgaccgcg cgttatgaag ctgcacgaga
     9541 gaaagtggcg caattactga atgcaccgga tgataaaact atcgtctgga cgcgcggcac
     9601 cactgaatcc atcaacatgg tggcacaatg ctatgcgcgt ccgcgtctgc aaccgggcga
     9661 tgagattatt gtcagcgtgg cagaacacca cgccaacctc gtcccctggc tgatggtcgc
     9721 ccaacaaact ggagccaaag tggtcaaatt gccgcttaat gcgcagcgac tgccagatgt
     9781 cgatttgctg ccggaactga ttactccccg tagtcggatt ctggcgttgg gtcagatgtc
     9841 gaacgtaact ggcggttgcc cggatctggc gcgagcgatt acctttgctc attcagccgg
     9901 gatagtggtg atagttgatg gtgctcaggg ggcggtgcat ttccccgcgg atgttcagca
     9961 actggatatc gatttctatg ctttctctgg tcacaaactg tatggcccga cgggtatcgg
    10021 cgtgctgtat ggcaaatcag aactgctgga agcgatgtcg ccctggctgg gcggcggcaa
    10081 aatggttcac gaagtgagtt ttgacggctt cacgactcaa tctgcgccgt ggaaactgga
    10141 agcaggaacg ccaaatgtgg ctggtgtcat aggattaagc gcggcgctgg aatggctggc
    10201 agattacgat atcaaccagg ccgaaaactg gagccgtagc ttagcaacgc tggcggaaga
    10261 tgcgctggcg aaacgtccag gctttcgttc attccgctgc caggattcca gcctgctggc
    10321 ctttgatttt gctggcgttc atcacagcga tatggtgacg ctgctggcgg agtacggtat
    10381 tgccttgcgg gccgggcaac attgcgctca gccgctactg gcagaattag gcgtgaccgg
    10441 cacactgcgc gcctcttttg cgccatataa tacaaagagt gatgtggatg cgctggtgaa
    10501 tgccgttgac cgcgcgctgg aattattggt ggattaatga cgaacccgca attcgccgga
    10561 catccgttcg gcacaaccgt aaccgcagaa acgttacgca ataccttcgc accgttgacg
    10621 caatgggaag ataaatatcg ccagttgatc atgctgggga aacagcttcc ggctttgcca
    10681 gacgagttaa aagcgcaggc taaagagatt gccggatgcg aaaaccgcgt ctggctggga
    10741 tataccgtgg ctgaaaacgg taaaatgcac ttctttggcg acagcgaagg acgtatagtg
    10801 cgcggcctcc tggcggtgct gttgactgcc gttgagggga aaaccgccgc cgaattgcag
    10861 gcacagtcgc cgctggcatt gtttgatgag ctgggattgc gtgcgcaact tagcgcctca
    10921 cgcagccagg ggttaaatgc gttaagcgag gcgattatcg ctgcgacgaa gcaggtttaa
    10981 gcacagaacc gggtcggata agacgtttgc gtcgcatccg acctgattgt ttacccctga
    11041 cgcgccgctt tcgccatcat cttcttcagc gcatgagaaa ccgcaacaaa accaaaggtg
    11101 gcggtcacca tcgttgccgc gccaaatcca gatgcacaat ccatccgctt tggtccttcc
    11161 gccgtggctt tcatcgcaca caccgtaccg tctgactgcg gatacaccaa tgcttcggta
    11221 gagaacacgc aatccacacc gagcttacct ttactgtttt tcactacgcc aaaatcgctt
    11281 ttcaggcgct cacgcaactt cgccgccagc gggtcctgaa tcgttttcgc cagatcggta
    11341 acctgaatct gcgttgggtc aatctgccca cccgcgccac cggtcgtgac cagcgggatt
    11401 ttattgcgcc gacaataagc aatcagcgcc gctttgggcc gtacactatc aatggcatca
    11461 atcacgtacg aataacccac gctcatatgc tgcgctacgt tatccggcgt cacgaaatca
    11521 tccaccaccg ttacacagca ctccgagtta atctggcgaa tacgttccgc catgacttcc
    11581 gcttttgcca gcccaacgtt atcgcgcagg gcatgaattt gccgattggt attggtgacg
    11641 cacacatcat ccatatcgat aagcgtgatt gcgccaatcc ccgtgcgcgc cagcgcttcc
    11701 gccgcccagg aaccgacacc gccaataccg accacacaaa tatgcgcgtc agcaaacagt
    11761 tgcaacgctt tttcaccata cagacgcgct gtgccaccaa aacgctgacg ccatgcatca
    11821 ctaattacca cagacatagc acctcagaat taaaaagggc gaggttatcc ctcaccctaa
    11881 acgcataaat gcctgatgcg ctacgcttat caggcctaca taactcctgc aatattttgg
    11941 atttgcatga tttgtaggcc ggataaggcg ttcacgccgc atccggcatg aacaacgcgc
    12001 acttagtcaa taatcaggat gtgaggttac ccctcaccct gtcatatccg taaaaacggc
    12061 atacagaata tcacatcagc cgctaaagac gttacctgcg cccggggcgg ttttcagcac
    12121 ccagacacgt ccatagtggt tgtaccaacc tgcgcggtgt ccggcttccg gcccgatccc
    12181 ttgataaata tcgaagtgtt ggcctttgat tgcgccaccg acatccaacg ccaccatcag
    12241 acgcaattcg tactgaccgt taaatttgcc gttattatcc agcagcggca cttctgccag
    12301 caaggttgta cctggtggga taatggaacg atcagaggca acagaagcac ggccaaccag
    12361 cggcaccgca cttgcccctt tcaccggtgc aaaagattgc ggtttaaaga aaacgaaaga
    12421 cgggttctgt tccagcagct cgcgaacctc ggcttcactg tgtgtttcgc cccaatgacg
    12481 aatcgcctgc atcgacatat cttctttttt cacttcgcca cggtcgatca gcaccttacc
    12541 aatgctgcga taggcatgac cgtttttccc tgcatagctg aaaaagttaa gcggactgcc
    12601 atcaccaaag tcgatatacc cactaccctg cacatccata atgaaattat ccatcagaga
    12661 gttgctgtaa gcgaggatgt acttatcgct caatgcaccc gcgtagatct ccgcacgaga
    12721 cggcagacga ccacgttttg gcggcatacg gtaaatagga tactggaact cgccctggcg
    12781 ggtatggcgc gcctgaatta ccggcgtgta atagcctgta aactgcacgt taccatagtt
    12841 gtcggcacct tccatctgcc aggcatcaat gccgaactgg cgcatattgc gggtatcacc
    12901 gcctgcgcgc agccactctt gcaccgcgtt ataaacatta ctctggttgc catacagacg
    12961 cggtgacgaa ttacggatat ggttaatttg ctcggcaaaa tcaccggcgt taatcggcgc
    13021 gccaacggca tctggctggt tcaccagaga gaaaggctgg gtaaatttcc cgtctttata
    13081 ttgctgtccg cgatcggttg gtttggaaga gcaggcggca agcattgcca caaccgtgcc
    13141 cataagaagg tactttaccc aacgtccttt cattgttctc ttcttcaggt taaaaaataa
    13201 ggcgcaacga agataacaaa ccaccgcgtt taatgaaatg agcagcctct ccctgacgca
    13261 aattttgcac aaaaaatagg ctttagtgat ttgtttttgt tcaaaatcat gccagatccg
    13321 tgatcggggt aaaaaaaagg ttgcatgaaa acgcgagcgg agtatagtgc gcatccacgg
    13381 acgcggggtg gagcagcctg gtagctcgtc gggctcataa cccgaaggtc gtcggttcaa
    13441 atccggcccc cgcaaccaat tattgaacac cctaacgggt gtttttttgt ttctggtctc
    13501 ctataaaaaa gcgccattca gcgccttttt atcatcccct tctcgccagg gtcgccccat
    13561 cggcaaaata cgctttaatt cccgcaagaa tagactccgc aacttcctgc tggaaggtcg
    13621 ccgttttcag tttacgctct tcctcaacgt tactgataaa cgccgtttcg accagaatgg
    13681 agggaatatc tggtgccttt agtacggcaa acccggcctg ttcaacttga tttttatgca
    13741 gcttgttgat tttaccgagc ttattcagca ccgctttacc aaacttaagg ctgtcagcaa
    13801 tggtcagcga ctgcaccata tcgaacatgg tgtggtcgac atagcggtca ccgcttttgc
    13861 tcacgccacc aatcaagtcg gaggcgttct gggtttgtgc cagatatttt gccgcagtac
    13921 tggttgcacc tttggttgag agcgcaaaca ccgaggaacc gctcggctgg cgactggtaa
    13981 aggcgtcggc atggatagag acaaacaggt cagcacgctg tttctgagct tttgctacgc
    14041 gcacttgcaa cggaatgaag atgtcttcat tgcgcgtcat gtacaccttc atattgccct
    14101 ctttctcgat cagagagcgc aaacggcgag ctatttgcaa taccacatct ttttcgcgcg
    14161 ttttgtattt ccccaccgca ccggagtctt cgccaccgtg gccagggtca agcataatga
    14221 caatcggacg atcgcgccct gctttacctg gttgtggacc actttgcgct ggcggcacct
    14281 gcttttcgag gtcgcctttg ttgtaatcct ccagcagcgc cagcagcggg tcctgcatat
    14341 cctgtgcatt ggccggatag aggtccatca ccagacgctc tttaaacccg gcgaccggcg
    14401 caagggcaaa cagctgcggt tttacgtttt gctttaattc aaaaaccatc cgtacggttt
    14461 gcgggtcaaa ttgcccgacg cgcgccgact tgatgaacgg gtcgtctgca cgaatttgtg
    14521 ccgccatccc cttgagtacc gagttcaggt ttacatcttc gatatccacc accacgcgtt
    14581 ccgggttact caacgcgaac tgcttatatt tcagctgacg atttgattct accgtcacgc
    14641 gggtgtagct ggacgcaggc cagacgcgca ccgccacgac ctggctgacc gcagccagac
    14701 tgacctgact tacgctcaat agccacatag cacccgcgcc ttgcagtaaa cgacgacggc
    14761 tgattgcagt gttggatcct gacatgcctc tcccgagcaa aagaaatcta agctgtgtaa
    14821 caagtaaacg actaatttga ccggaaactt tagcgaaaga cgcataatct gtcatctaat
    14881 aaacggtaaa cattcttttt atattcacgc cattactgat aaaaaagtcg ctctcgcata
    14941 aaatttacac ttgcaccctg cgaaaaaaca gaataaaaat acaataaatt cgaataataa
    15001 tgcaaagagg tgtgccgtgg taaaggaacg taaaaccgag ctggtcgagg gattccgcca
    15061 ttcggttccc tatatcaata cccaccgggg aaaaacgttt gtcatcatgc tcggcggtga
    15121 agccattgag catgagaatt tctccagtat cgttaatgat atcgggttgt tgcacagcct
    15181 cggcatccgt ctggtggtgg tctatggcgc acgtccgcag atcgacgcaa atctggctgc
    15241 gcatcaccac gaaccgctgt atcacaagaa tatacgtgtg accgacgcca aaacactgga
    15301 actggtgaag caggctgcgg gaacattgca actggatatt actgctcgcc tgtcgatgag
    15361 tctcaataac acgccgctac agggcgcgca tatcaacgtc gtcagtggca attttattat
    15421 tgcccagccg ctgggcgtcg atgacggcgt ggattactgc catagcgggc gtatccggcg
    15481 gattgatgaa gacgcgatcc atcgtcaact ggacagcggt gcaatagtgc taatggggcc
    15541 ggtcgctgtt tcagtcactg gcgagagctt taatctgacc tcggaagaga ttgccactca
    15601 actggccatc aaactgaaag ctgagaagat gattggtttt tgctcttccc agggcgtcac
    15661 taatgacgac ggtgatattg tctccgaact tttccctaac gaagcgcaag cgcgggtaga
    15721 agcccaggaa gagaaaggcg attacaactc cggtacggtg cgctttttgc gtggcgcagt
    15781 gaaagcctgc cgcagcggcg tgcgtcgctg tcatttaatc agttatcagg aagatggcgc
    15841 gctgttgcaa gagttgttct cacgcgacgg tatcggtacg cagattgtga tggaaagcgc
    15901 cgagcagatt cgtcgcgcaa caatcaacga tatcggcggt attctggagt tgattcgccc
    15961 gctggagcag caaggtattc tggtacgccg ttctcgcgag cagctggaga tggaaatcga
    16021 caaattcacc attattcagc gcgataacac gactattgcc tgcgccgcgc tctatccgtt
    16081 cccggaagag aagattgggg aaatggcctg tgtggcagtt cacccggatt accgcagctc
    16141 atcacggggc gaggttctgc tggaacgcat tgccgctcag gcgaagcaga gcggcttaag
    16201 caaattgttt gtgctgacca cgcgcagtat tcactggttc caggaacgtg gatttacccc
    16261 ggtggatatt gatttattgc ccgaaagcaa aaagcagttg tacaactacc agcgtaaatc
    16321 caaagtgttg atggcggatt tagggtaagc gatgaaaatc gtcggatgcg acatgcgtaa
    16381 cactcgtacg tcgcatccgg caattacgtt tattcccgtg aactaaacaa cgccgccaga
    16441 ccactgcgcc gctcagtacg agtggcgatt gccgcactta atatgcgctc atcggcatac
    16501 agcgacagac ggcgacgcgc gcgggtcacc gcggtataaa ccagctctcg cgttactacc
    16561 ggcgtgcgtt ggctcggtaa aatcaacgcc gcatggtcga actccgatcc ctgcgattta
    16621 tgtaccgtca tcgcccacgt agtttcgtgc tctggcaagc gactcggttg cacagactta
    16681 atattgccgt ccggcatcgc aaaccagacg cgcgtcccct gcccgcgatc cagcgcaata
    16741 ccgatatcgc cattaaacaa cccaagcgcg ctgtcattac gggcaatcat caccggtcgg
    16801 ccttcgtacc aacgagagtg cggattacga tgaattttgc gcttctgttg cataaactgc
    16861 tcaattcgct cattcagtcc agccacgcca aatggccctt cccgcagggc gcacaaaagt
    16921 tggtactcat tgaacgcctg aatgattaaa tccggctcgg cacgcgcttg cagcagatcc
    16981 agataacgtc cataacccgc aagagcttcc tcaagcatcg caatataatc ttcgccgctc
    17041 tgtaaaagcc gtttttcaat atcagtaaaa tcctgctgaa aaacggtttt cactgccgtt
    17101 ttatcaccac ggttgatcgc cgcagctaac tgaccaatgc cagaatcgct gccgaaacga
    17161 tagctttttt gcagcaggca gagactgtcg cgcaaagatg ccgcttctgt gccagttcct
    17221 gccggaacgt gagttcccgt caggcggctt agctgcccgg cacgctcggc ggtaaagccg
    17281 gcattggcat aagcgcagat atcgcccagc acagccccag cctcaaccga ggccagttga
    17341 tcacgatcgc cgagaaagat cactcgcgca tgatcgggca aggcgtcgat cagtctcgac
    17401 atcataggca gatcgatcat tgacgcttca tctaccacca gcacatcaag atgcagcggg
    17461 ttaccggcat gatgacgtaa acgctggcta cccggctgcg cacccagcaa tcggtgcaaa
    17521 gtactggcat cttccggaat acgtttcttt tgttcatcgg tcagcggtaa ctgtcgcaaa
    17581 gccttgccga gagattcggt taagcgcgcg gcagctttac ccgttggtgc agccagacgg
    17641 atacggcagc gttcgccgtc ggccatttga attaacgctg ccagcaactt cgctacggtg
    17701 gtcgttttac cggtgccagg gccgccggaa atcaccgaga tccgccgcgt cagcgccact
    17761 gctgccgcca ctttttgcca gttaatttca tcaccagtcg gaaagagttt atccagggtt
    17821 tgcgccaaca aggcttcatc cacctcgatg gcatgattca cctcattgaa gaagcgcgct
    17881 accgtccgct cgttacacca catgcgattc aaataaagac gatcgccaca gaggatcatc
    17941 ggcgtgggtt catctccccg gctgaccgct tgtgaagcca gcaagcattc ttcccaattt
    18001 tgtagctcac cgatttcact gacacaggtc gccaacagcg gatgcgactc ctcattattt
    18061 tccagtcgtg aaagcggcaa acaaacgtgc ccctctccgg catcatgact taacagtgcc
    18121 gccgcgaggg tgacggcagg atgttcatct cccgccacgg tcagggcaaa ctgcacgtcc
    18181 agcgggcgta gctgtttgtg ctccacagct tccagtaatt gcttttgcaa tttcattacg
    18241 cctcctccag agtcataccg gcaaacatct catccatcag gtcaatcaac ccggcgttgg
    18301 ggcgggttgt gtaaatccct tgttgcggat gttctttatc aacaccacgc aggaacagat
    18361 aaataacgcc gccaaagtgg cgctcatagt cgtaatcagc aatgcgatgg cgcagataac
    18421 ggtgcagcgc cagggtataa agctgatatt gcagatcata gcggtgtgcc tgcattgccg
    18481 ctgccatagc ctgttgggtg taagccgaac tatcttcacc caaccagttg gatttatagt
    18541 cgagcaggta ataacgccct tcgtggcgga acaccaggtc gataaagcct tttaacatgc
    18601 cacgtgcctg catgaactcc agcggcgggc agccagcgga tagcgggtca aactggcgaa
    18661 ttagtgcatc aagctggctg gcgatgagcg gttcgctaat cggcaggtaa aactccatct
    18721 ccacctgttt atcacggtcg gaaagctgac tcaggctaac acccgtttca ttgagaggtg
    18781 cctggaggac agccgtgatc cactcggtca acaccggttc ccactgcgat tcaaagccgc
    18841 cgagttccag tttttcctgt acccagttcg ggtcaaccgg ctgggtaaaa ttgaggtctt
    18901 caaacaaact gtgcaagaat gtccccggtg acgcaccgcg cgggaactga tgcggtgtta
    18961 acgtcggttc ttcaacgacg ctgaccacgc ctgcggcatc aacatccagc cgtggcatca
    19021 gatcctgagc gataccgtga ccacgctgct gcaagccgga gtagctggtg acacgccagt
    19081 tatcgccggg caatcgttgt agcgtcctcg cattcagttc tgcagttaaa gcatcattaa
    19141 cctgccaggg ctggttatca ccagtttgtg ccgtttgcca ggcaatatca tcatcgcata
    19201 acgcttcaat acaggtgcga agccctgccg catcttgcgg ttcccctttt tgcagcaaac
    19261 gcccgagggc actttggtgg acgtcggtgt cacctttttt atcgccacga cggcgcacca
    19321 gcggtgcaac gccgagactg caatgccaaa ccgaacgcgt cagcgccacg taaagcaaac
    19381 gcagatcttc cgccagacgt tcgacctccg caagatcgac gctttctggc gcagcgttaa
    19441 gatccagaac agcctcaaac gagtggcgat cgtgataaaa cgcctgatcc tggacgcgga
    19501 aattggtaat aaatggcaac cagaccagcg gatattccag cccttttgat ttatggatcg
    19561 tgacaatctg taccagatgt ttatcacttt cgaggcgcat ttgttggctg gaggcattac
    19621 tgtctggctc gaggatatgt tgcgataacc agcgtaccag cgcatgttca ctttccagct
    19681 gcgttccggc ttcttgtagc agttcgctga tatgcaagat atcggtaaga cgccgctcac
    19741 cgcctgccgt tgccagcaag ttttctgcaa tgttacgcgc cgacatcagc gcccgcatca
    19801 tcggcataac gccacgtttg cgccagattt gccgataacc atcgaactct tcgaccaccg
    19861 cgtcccacgc atgttcgtca ttattcagcg tttcgatatc cagcgcgttc agccccatca
    19921 ttgacgttgc cagcgcacta cgcagggtgt tctcacgttc gggcgtcatc accgcctgca
    19981 acaaccaaag catttcctgc gcttccagag tttcaaaaac actgtcgcgg ttcgaaaggt
    20041 aaacggaagg gatttccagc aacgttaagg catcgcgcac ctgggcggcc tcctggcggc
    20101 tgcgcaccag cacactgatg tccgaagcac gcaccggacg cgcgtcgtcg ccgttcatca
    20161 gcaacgcttc gccccgctgt ccggcctgta gccagtcgcg aatttgcgca gcacttacct
    20221 gcgccatggt actttgatag tcgccaacgc cgcaactttc gccttccatc agccacattt
    20281 tcatcgcagg ctgcgtttca cctttaaaga caaaacgtaa cgcctgattt ttcccggctg
    20341 atttcactgg aataaacggt atttcgcgaa acatgaacgc gtcatcagtc tggctgaaaa
    20401 gcttgttcac actgttcacc attccaggtg cggagcgcca gttggtgtct aaagtgtagt
    20461 gggcgtgaac ttcactacgc gccttcatat aagtgaagat atccgcaccc cggaatgcat
    20521 atatggcctg cttcgggtcg ccaatcagca acaatgcggt ttccggctga tggtgccaga
    20581 tacggcgaaa aattcggtac tgctgggggt cggtatcctg aaattcatcg atcattgcca
    20641 ccgggaatcg cgtacggatc gccgctgcca acacctcacc gctttcgcta cgcagcgcgg
    20701 aatcgagccg acttaacatg tcatcaaaac ccaattcgcc acggcggcgt ttttcacgcg
    20761 ctactgtttc gcggatctca gccaatgcgc gggtgatcac cagatcgcgg atcgacaatg
    20821 gttctgcaag cagttgatcg atcgcctcaa acagtggatg tcgcggggtt tcccccccgg
    20881 ccttcgtgcg atcttctaag aaacgctggg agaatttttc cagcgactcc ggcaactgat
    20941 aactgtttct ctcttcttct gcccaggcgc tgatcttgtc gatccattta gcctgattgc
    21001 tacggttaaa cttgcgtcga tcaataccag aagattcgat cagcgcatcc agttcaccca
    21061 ctgcgtcgcg ccactgctgt tttaccgtat caatacgcgc cacaatttgc gcgtgacggg
    21121 aagccagcgt ttcatcatcg ggcggcggtg ctttgataac cggcgcttca ccttgcagat
    21181 aacgattaat atcgcgcagc aacgcctgcg gccctttcca ggtttcaaag acgacctggg
    21241 ctatttcacg cggcagcggg tagcagtggc gacgccagaa atcggcgcag gcctggtaac
    21301 gtagcagaga ctcatcttca atcagctgct gctcaaacag catgccggat tcaaaggcat
    21361 tcaggttgag catgcgctgg caaaaaccgt gaatagtaaa gactgccgct tcatccatct
    21421 gccgttcggc taacaacaac cacttcgcgg cttgcgcttt atcgtcgatc tcttccagca
    21481 gacgttcgta cagtggattg tcggtggttt cacgcagaca ggcgatgcgc aactcatgga
    21541 tattgctacg gatacgacca cgcaattctg ccgtggcagc ctcggtaaag gtgaccacca
    21601 gcagttcttc aacggtcagc gggcggggaa aggcggcgga accgcctagt ccaagtaaca
    21661 ggcgcaaata gagcgccgca atcgtaaagg ttttgcctgt gccggcagag gcttcaatca
    21721 ggcgctcgcc ctgaaagggc aagcgcaaag gatctagtgt ctcggcgaca tcactcattc
    21781 ttttcactca tcaggggcat tgtttgctgc aacgcgctga cgttctccca cactttccag
    21841 ccttcaggat gcacatattc ggctttcccg ttctggctgc cggaaatctg cgacagaata
    21901 gccatgcctt gcggctcgac caccgcctga tggaagaaat cagcaagttt ttgcggcgtc
    21961 agcagtttta tctgggccac gattttatca cgcgaatcga agcgcatatt gccgcgatcg
    22021 aaatctttac ttaacttcaa tgcttcttcg ccgagcgttt gcggtgcctg cagcatctgg
    22081 gtaattaccg cctgctggat ttgcgcaaac tcatctggct tcatcgctcg caattttgcc
    22141 tctgcggttg ggaaaaacgc cttgtaacgc tcccacaaga atgaaggctg tttatcattg
    22201 ctttgcaaaa ggaagcccat gccccactga cgccccacgc tcattggaaa cgcaaacacg
    22261 gcatagccca attgttcttc ggtacgcaac tgattgtaga accacggctg tacgatctgc
    22321 cccaacagag agctataggc tgagcttgtg tattcatcgt agccagtcgg tacaaatacc
    22381 gctgccagtg cggagtcggt gctgttaccg gctttttcaa agatgacgga ttgtttttta
    22441 tcgaccacta catctttgtt tcgacaccac tctgaaccat cagcgcccaa ctgtttttgc
    22501 acatcgcgtg ccagcgttgt tgcctgggcc tcggtcatgt tgccgataac cataaactct
    22561 ggtcgagccc ctgattttaa ggcgtcacga taagccagca cctctttcaa cgtaatggag
    22621 ggcaaaattt tacgccgttc atctcgcgag aagtacggca cttgcgagag catctgcgcg
    22681 ggcataatcg cctgctcaaa cgctttaccc ttttctgcgg aatccatcat ctggttatac
    22741 caggacttcg cctgctcaag ctgatcttcc gtagcggtat agctaaagta tccctcgagc
    22801 aatgcctgga acagctgcgg cagacgctgg gtgtaaccat tagcattaac cataaggccg
    22861 ttgttagcgt tggtggaaaa acttatgcca ccaaccgacg cctggttgct taactgatca
    22921 agcgccagcc ctgcgagata atcattgagc gcaaacatca cctgattgcg ggcgctgtcc
    22981 atggctttcg gattacgcaa aatcaggctg acatcagctt tgggttcgct ggaaaaataa
    23041 cggcttggcg catacaccac gcgcagattc gactcatcaa caatcagctc tggatggtca
    23101 tatttcttct ctgacttaat cagcgagaaa tcgtcaggaa tataggggtt aagctccggt
    23161 aaggagagcg caatattggc agctttttgc tgccagtcag cgaaagtttg ttcgctgatt
    23221 ttatcgacct gatacggcgc atcgacaaaa taagccgttt tattgtgcgg ctctttcggg
    23281 ctgatatacc agatacgcgc attctgcggc gtcatcatcg ccagacgttc ttttactgct
    23341 ttagcatcgt accgatcggc aatattgact gcatccagcg tatgctcaac aggaacgcga
    23401 atcatggtat ctgccagcca ttcgacgtaa tccatatcac gggtgattga cggataacgg
    23461 aagtcgatat ccagcacatt cgccagttca tcgaagtatt gtttatcgat ccctttttca
    23521 cgtaacaaat tgaggtagct aaaaatagcc gccacaacct gatcgcgatt cgccagacct
    23581 ttatcggtta aagacgcaga gatcgctaat acgccgctgt tgccgttgac gataggatcg
    23641 gagttggcgc taatgccctc aactaacccc tgcttttgca gccagtcaga cagtgtacct
    23701 gggctgcgat taccaatcag ataggtaatc aattcatccg ttttactgcg gaacttcgct
    23761 gagttgttat cgatgcgaaa ctcaacgcgc agaactttac gcggcagcgc tgggacgtaa
    23821 tgaatgataa tgcccttttg cgcgtcggtg actaccggca cggtgatttc cggttttttg
    23881 ctctctttgt tcggcacgcg accaaaagtg tccgccgcca tttttgccaa ctccggcagc
    23941 ggtttattac tgtaaataac cgccttcatc aaattggcgg aatagtactt ctcgtggaaa
    24001 tctttcagcg cctgctgcac cggattacca ggtttgtcgc ttaaagtttc gaggttacca
    24061 ccagaaaact ttgaaccggg gtgtgccggg ttaatggttt ctgcgctgac ctgtgccatg
    24121 cgcatcccgt cacgtgtacg cgccatggtt aattcagcgt tcactgcatt acgctcacgt
    24181 tcggcgtatt tcttgtcgag caagggttct gcaatagcat cggccaggcg gtctaccgca
    24241 ccgggcaagg cgtcgttctc aacttccaga tagaaagccg tgcgatacgg cgcagtgctg
    24301 gcattgtgac taccgccgtg cattttgaga tattcggcca gactgtcagc ctgcgggtac
    24361 tttttcgacc ccatcagact catatgttca aggtaatgtg ccagcccctg gtacgcctcg
    24421 ggatcttcca gcgacccaac gggcaccacc agcgccgata gcgatttaac tgcctgcgga
    24481 tcagaaacca gcaagaccac cataccgtta tccagacgta tagcctgata ctggcggtta
    24541 tctttatcac ttttacggat ggtttcctga atcggctgcc atcccgtttc tgcctgactt
    24601 aagggtgccc aaagggcaac taacaacaat aatgctttga accaggtgct gcggggcatt
    24661 cacggacctc ataatcaact ttattttctg gccagattca acaggttaac gcaaatcatc
    24721 tgccagaatt taatcttgtg ctgcacgagt cagcctatgt ttatataacc atcagtccgt
    24781 gactggtgcg catcataaag taagcggata gattgcgcaa tttttataca gcgctcatga
    24841 ctgattaaag cgaaacagcg gtaacaggaa acgttgcgac tgttcaacga ttgcctccat
    24901 tgtctctggt gttaattgcc gccagagcct ttgataccag atatcatcac cttcgccact
    24961 caccatcatg ttgccttcgt aagcctgaag gaatttcgta cgggcttttt gcaacgtgga
    25021 atcgtcatcc agcatggcat cgttttgcgc gtcataacag gtttttagcc acgcgccgcc
    25081 actttcaggt aacaccagca atggcgcgga catcccttca cgatacccct caatcagttg
    25141 tgagaggtaa tgcaaagcct gttcggctgc aagcggcgga aaacgccact cgccgtcttt
    25201 gcgtagaaaa aggcgacttt caccattacc accgctggca cagtagacaa ggtgttccag
    25261 ccaaagttgc attccctgcg ccacacttaa taaagaggga cgccagcgca acaagccatc
    25321 cggctgcacc tgcggcaacc agccagttat ctgcacaccg ttgcaagcga gatcaatttc
    25381 catactctgc cccggctggc gacaggcaat gactctgtcg gcaagctgct gcatctcctg
    25441 gcactgtgtt tcccagaaaa tttcaccaaa agcgccatac ggtaaatccc ctgccgcccg
    25501 gaagcggcgg aacaagcgtt cggcatcatc ctgctcaacc agtgcattca ataactgctg
    25561 attgatttga taacggctaa gtccttccag aataaatggc tcggtgtcgg ggatttcgct
    25621 gtcttcagta cggaagttca cctgcaaacg catctggaag aacgcccgca ccggatgtgc
    25681 ccagaatcgt tgtagcgttt ccagcggcac ggtttccggt aaggtaaacg gcagcggctg
    25741 aacaaattca gaatgtgctt taccagcctg gctggccgca ggtagccatt cacgagcata
    25801 gctttgtcgt tcgcctggct ggtagttttg cggatcaaac ggcatccggg tatggaggca
    25861 agtaagatgc gcttttaccc ttgcctcgct ttcatcacag ttgagcgctt catcgcccgg
    25921 tagataatga ctttgcccga tgtagtcgat cagttcctgc accagtaccg acgggaaacg
    25981 ttcactgtta tcctgaatgg aacgcccgat atagctgata tagagttttt gctgcgcgga
    26041 aattaacgct tccaggaaca ggtagcggtc gtcatcgcga cggctacggt cgccacgctt
    26101 cggtttctgg ctcatcaggt caaagcccaa tggcgcaagc tgacgtggat aaacgccgtc
    26161 gttcattccc agcaggcaaa ccactttgaa cggaattgaa cgcattggca tcagagtaca
    26221 aatgttaacc ggcccggcga gaaaacgctg gctgatacgt tcttgatcca ggcgctgtgc
    26281 cagttcatca cgcaatagtg acagcggcac cgcatcgcca tactgcgcac ctaaaccttc
    26341 ggcgataatc gcctgccatt gttgttcgat cagcgtcatc gccgcttcgg tttccgcatc
    26401 cggcaggaag agggcgttga gcatatcgcg acaaaccggc aaccactctt ccagcggacg
    26461 ctcctgtgcc agcccgcgac gccagatgtt tagctgcatt agcagtgaag ccagatgccc
    26521 caccagttct gcaattaagc cgctcgattc atcataaggt agaaccgatt gccactcgcc
    26581 ctgcgcgctc tccatcgcgt agcccaacaa catacgcgtc aggccaaatc gccaggtgtg
    26641 ttgtccggtg gcggggagtt ccagctcgcg aacgttgtcg tcatctatcc cccaacgaat
    26701 gccggattcg ttgacccact gacgtaaata acgcagccct tcttcggtga tgtcaaaccg
    26761 cgccgccagc accggcacat ccagtaatgc cagcacgtct tccgacacaa agcggctgtc
    26821 tggcaacgat aacaggctga taaacgcttc aagtacagga tgcgactgcc gcgcccgacg
    26881 gtcggaaatg gcgtaaggca ggtaacgatc cgcaggtgcg ctaccaaaca cagcctgaat
    26941 aaacggactg tagctgtcga tatcagccac catcacgatg atgtcgcgcg gagtaagtgt
    27001 cgggtcttct tccagcatcg ccagcaggcg atcgtgtaaa acttcaactt cacgctgcgg
    27061 gctatggcaa acgtggaagg tgatactgct atccagtgga tcaagcggac gtttgttatc
    27121 gctacgggaa aactcttcga tgttcacacc agcaacggcg cggttttcca gttccagaat
    27181 gtcagactga atgttatgca gcaggttatc tggcgtcaca tcgacaaagg cgtccagctc
    27241 ctggctgctc tccaggtcag aaaggagata aatgtagtcg cgcccaagct tgccccatga
    27301 ggccagcagc gggttgccga catcctgttc accatcgctg ttaaagagct gcccggcatt
    27361 ttcgctgtcg cgaaatagcg gtaattcgcg atcttcaaaa ctgtgtcggc gctggcgagt
    27421 cagtagtttc gccagataag caggatcttt aatgtcgccc cagtaataac ggcaggggtt
    27481 ggtaaacagg agatggattt caatatgttt acccagcgcc tggagcgcct ggagataaac
    27541 aggcggtaac gcggaaatac cgcatataaa gacgcgcgaa ggtaaccccg gcgggcaggt
    27601 cgtcgcggac tccagcgttt cgataaagcg ctgatagaga ttggcgcggt gccagcgcgg
    27661 ttgtccgagt tcatgggtat attccaccag cgccttccac aacggcgctt gccaggcctg
    27721 tgcctctccc agcccttcaa ccaaatgtcc tgtttcccac tgtgccagcc agtccggacg
    27781 atagaccaga tactggtcaa acaggtccgc cgcttttgag gaaagctgga acagctttcg
    27841 cttgtcgcta tcgtcagtca gataatgccg caacagggta aagtcttcgc gctccagcaa
    27901 ttgcggcagc agagtcatca gtttccagct catgctctgt ttgttaaagg cgctctcttt
    27961 ggggatctcc ggtaacaccc ggacgaacat atcccagata aagctcgcag gcagcggaaa
    28021 atcaatgttt gccgcaatac caaacttttg cgacagggtc atttgcagcc actgtgccat
    28081 accggtactt tgcaccagaa tcatctctgg ttcgaaagga tcgtccagcc gttcgcgttc
    28141 gacaataaac tccatcaacg cttccagcac gtccagacga ttggaatggt agacccttaa
    28201 catagcggct cctgactact gacgattcgg gcaatgcagg cgggtcatct cgcctgctct
    28261 gcccccgggt gaaactagcg taacgctgat gctgacacat cccgcctgcg atgtctgcat
    28321 tcggttgacc tgccagttgg caggtggcga aatcgcgcgc aattgcgttt gctgccaacc
    28381 atgccgccag agctgttggt actggtttct gctggtaaga ctgttcatta atgttcgctg
    28441 gaaacccgat aacgcagtga caatcatcac catcaacacc atcgccaaca ttacctccgg
    28501 caggctaaag ccttgttgat tcttcaggga agctgacata acgccccctc tttcagcgga
    28561 caaaaatcgc tccagccgcg tggcgaaaaa acaatttttc catcaatgac ttcgcctgtt
    28621 cgccacaacg aaacgccttc ataaccggca attaataagg cttcattatc ttccagtaaa
    28681 cgcaaacaaa cccgggcacc agtggcagcg tacagtaagc actgaactgc gggctgcgtc
    28741 tgccaggagt gcattttccc ccacgccaac gccgactgaa cgatggcctg gcggcgcagt
    28801 gactgacttt ccatgctcac gcgagaggca aaactgcgat cctgctggct cattccttgt
    28861 aatagcaagc tacccaaaac cagcagcatc aggaccagag ccagtgacga aacacccttt
    28921 tcgcggttca caggttgaat cctgtcacgc tatagctggc atccaccacg gtttgcggtt
    28981 cagacttact ggcagcacgc atattaaccg tcaacaccgg cgagaagccg ctgacatcct
    29041 gacgtacgac ctgaaaagtg tcgataatga tggcatccgg attagtgact ttatcccagc
    29101 ccttaccttc acaggatgtc gcaccgcgta gcgtttccaa cacttgctcc ttcagacgaa
    29161 atccaatctg gtcggactct tttaccggtt cgcgatccca gataccgtta ctgttcgcat
    29221 cccactgcac aatgatacag tcaccctgtc cgacaatttc cagcccttcg ccggtacaga
    29281 tgccatgaca ataacccgcc ctctggagat gcttcgcgac ggtaaatacc cgcagccaga
    29341 tttcatcttc cagtgccagc ttacgggtgc tcgttaaact ttcacgctgt aacgcaggca
    29401 gaaagcgtgc agcccccaac aacaatacgc tactgatcgc catagcaatc aacacttcca
    29461 gcagagaaaa accttgctct tttacaggca tccttctgtt tctccttgct gacaaagccg
    29521 gagtcttccc cacggcgaaa ccaccagcca ccactcgccc gttgagtttt tgaagccaat
    29581 atgcccggcc catgcggtat tgcgcaggcc aaagaaagca agcgaaggtg tcaggtcgct
    29641 catttcgact tcgggccagc gtggcacaaa gaccaatggt gaactgccat gacaggtatt
    29701 cgccccagca acggaactca caaggcacca taacgtcccc tccctgataa aactgatact
    29761 gtggtcgcgg ttatgccagt tggcatcttc acgtaaatag agcaaatagt cccgcgcctg
    29821 gctggcggtt tgccaaagcc gttgcgactg ctgccagtat tgccagccat agagtccact
    29881 tgcgcttagc atgaccaaaa tcagcatcgc gaccagcgtt tcaatcagcg tataaccacg
    29941 ttgtgttttc atgccggcag tatggagcga ggagaaaaaa agacgagggc cagtttctat
    30001 ttcttcggcg catcttccgg actatttacg ccgttgcagg acgttgcaaa atttcgggaa
    30061 ggcgtctcga agaatttaac ggagggtaaa aaaaccgacg cacactggcg tcggctctgg
    30121 caggatgttt cgtaattaga tagccaccgg cgctttaatg cccggatgtg gatcgtagcc
    30181 ttcaatctca aagtcttcga aacggtagtc gaagatggat tcgggtttac gtttgataat
    30241 caacttcggc agcggacgcg gttcacggct taattgcaga tgagtttgat ccatatgatt
    30301 actgtacagg tgcgtatcgc caccggtcca gacaaaatca cccacttcca gatcgcactg
    30361 ctgcgccatc atatgcacca gtaacgcgta gctggcaatg ttgaacggca ggccgaggaa
    30421 gacgtcacag gagcgctgat aaagctggca agagagtttg ccgtctgcca catagaactg
    30481 gaagaatgca tggcacggtg ccagcgccat tttatccagt tcgcctacgt tccacgctga
    30541 aacaataatg cggcgggaat ccgggtcgtt tttcagctgg ttcagtaccg tagtgatctg
    30601 gtcaatatga cgaccatctg gcgttggcca ggcgcgccac tgtttaccat acactggtcc
    30661 gaggtcgccg ttttcatcgg cccattcgtc ccagatggtg acattgtttt cgtgtagata
    30721 agcaatgtta gtgtcgccct gcagaaacca cagcagttca tggatgatgg aacgcaggtg
    30781 gcaacgttta gttgtcacca gcgggaatcc atcctgcagg ttaaaacgca tctgatgacc
    30841 aaaaatggaa agcgttccgg ttccggtacg gtcgtttttc tgtgtgcctt cgtcgagcac
    30901 tttttgcatc agttctaaat actgtttcat ggttcctcag gaaacgtgtt gctgtgggct
    30961 gcgacgatat gcccagacca tcatgatcac acccgcgaca atcatcggga tggaaagaat
    31021 ttgccccatg ctgatgtact gcacccaggc accggtaaac tgcgcgtcgg gctggcggaa
    31081 aaactcaaca atgatgcgaa acgcgccgta gccaatcagg aacaagccag acacggctcc
    31141 catcgggcgc ggtttacgaa tatacaggtt gaggataata aacagcacca caccttccag
    31201 cagcagctcg taaagctgtg atgggtggcg cggcagcaca ccgtaagtgt cgaaaatgga
    31261 ttgccactgc gggttggttt gcagcagcaa aatatcttct gtacgggagc ctgggaacag
    31321 catggcaaac gggaagttcg ggtcaacgcg gccccacaat tcaccgttaa taaagttgcc
    31381 caaacgcccg gcaccaagac caaacggaat gagtggtgcg ataaaatcag aaacctggaa
    31441 gaaggaacgt ttagtacggc gggcaaagat aatcatcacc acgataacgc caatcaggcc
    31501 gccgtggaaa gacatgccgc cgtcccagac acggaacaga tacagcggat cggccataaa
    31561 ctgcgggaaa ttgtagaaca gaacataacc aatacgtccc ccgaggaaga cgccgaggaa
    31621 gcccgcatag agtaagtttt caacttcatt tttggtccag ccgctgcccg gacgattcgc
    31681 ccgtcgtgtt gccagccaca ttgcaaaaat gaaacccacc agatacatca ggccgtacca
    31741 gtgaagcgcc acgggtccta ttgagaaaat gaccggatca aactccggaa aatgcagata
    31801 gctactggtc atctgtcacc acaagttctt gttatttcgc tgaaagagaa cagcgattga
    31861 aatgcgcgcc gcaggtttca ggcgctccaa aggtgcgaat aatagcacaa ggggacctgg
    31921 ctggttgccg gataccgtta aaagatatgt atatgatccg cgctataacc ctccgcgaat
    31981 cagcccgccc atgccgcgac gctccataaa ggctgcaacc tgatggcgaa cttcggtcgc
    32041 cagttgcgct tccagactac gctgcgcaag attttctgct tcggcataat caatgcgccg
    32101 cagcaggtat tttacccgcg ctacagaacg tccgttcata gacagatggc gatacccaag
    32161 cccaatgagg attgccacgc acatgggatc gcccgccatt tcaccgcaca aacggagatc
    32221 gattccatgt atttccgctt cccgggcgat catcgccaga gctcgtaaca ttgcaggatg
    32281 aagactgtca taaatgttcg ccacccgggt attgttgcga tcaacggcca gaatgtattg
    32341 agtcagatcg ttggtgccaa cagagatgaa atcgacccgc tttgccagat gcggcagcat
    32401 aaataccatt gacggcactt ccagcatgat gccgatacgt ggtttgggaa tttcgtaacc
    32461 gatcatctcc tcgacttcac gtccggcacg ttcaatcagg cggcgtgctt cgtcaacttc
    32521 atcgaggctt gtgaccatcg gcaacagaat attcaggttg cccgtagcgg cattagcacg
    32581 cagcatcgcc cgcacctgga tcaagaagat ctccggctga tcgagcgtaa tgcgaatccc
    32641 acgccaaccc aggcatggat tctcttcgct aatcggcatg taaggcagct gcttatctgc
    32701 tccgacatcc agcgtacgca aggtgacggg tttatcattg aacatttgca gcatcccctg
    32761 atactgcgcc acctgttctt cttccgacgg aaaaccactt tgcagcatga atgggatttc
    32821 agtgcgataa agaccgatgc catcaatacg gctgcccagt ttttcttcat gttccgggct
    32881 taaaccagca ttgagcatga cttttatacg ctcaccgctt tttaactgcg cgggtaaatt
    32941 gacgtcatct tccgccagac ggctaagctc aatctcttca ctaattagcc gctgatattc
    33001 ttgcagcagt accggctccg gatcgaccag caattcaccg cgatagccat cgacgatcag
    33061 cgtccgacga tgcagcaccg aaggctgaat atccgcgccc atcacggtag ggatccccag
    33121 cgcacgtacc atgatcgcag catgggagtt ggctgcgcca tctcgcacga caacaccgac
    33181 taagcgatcc tggggcagct cagcaagagt tgtcgctgac agttcatctg ccaccagaat
    33241 gaaacgttcc ggccaggcgt tcggcccttg attagcgtca tcaagatgaa acagcaatcg
    33301 ctgacccagc gcacgtaaat cgccagcccg ctctttgaga tagttatcgc ttagcgcggc
    33361 aaactgttcg gcaaattttt caatgaccgt ttttaccgcc cattctgcca ccgagccttt
    33421 atcaacctcg gcaaacaatt cgcgacgcag ccgggtgtcc gaaagcaggt gcgagtaaag
    33481 atcgaaaata gccgccgttt ctttttgtgc accggcggca aagcgtttgc tgtagcggcg
    33541 aaactcgttt gccgcttctt ccagcgcccc ggtcagtcgt tcgcgttcca gagccggatc
    33601 cagcgttgat gcctgataca cctgttccat taaaggtaac gtggcatcct gccagccttc
    33661 ggcaatcgct acaccaggtg ctgccggtaa tgcgcggatt cgcgtctggc gatattgccc
    33721 aaacaaggca gtcaactgcg actgagaaag aatcgctgcc atctgggtgg caagcgtcac
    33781 caggaaggat tcttcacttt cgtcatactg gcgcaactct cgttgctgta ccaccagtac
    33841 accaagcaac tggcgacgtt gaataattgg tacgcctaaa aacgcgcgga aacgttcttc
    33901 ttttacggag gggatgtatt tgaagctggg gtgcttttgc gcatctgcaa ggtttatcgg
    33961 ttccgccagc ctgccaacca ggccgacgat cccttcatca aaggcgagcg ttacagtgcg
    34021 accgcgtggt tttttcagcc cccgggtcgc catcaggtag taacaacgtc gatcatgatc
    34081 ggccaggtag accgaacaga cctcggtatc catcgcaaga cagatgtcgg taaccagaat
    34141 atttaacgcc tcattcaggc gtggtgcgct ggctaccttt tcgactattt cgcgcaggcg
    34201 agtgagcata attggcgtga cttaacctct tttacgtcga taagcagatg cgttttgtgg
    34261 ttttggcgta ttttcctgca gtgacatcac cacactcgcg aactctttca ttaccctacg
    34321 gtagacatca cgtttaaatg acaccacctg tctgaccgga taccagtaac ttacccatcg
    34381 ccagccgtca aactctggtg tactgctggt ttgcatattg atttctgcat cgccgctcac
    34441 cagctgcaag agaaaccatt tttgtttttg gccgatacaa accggcttcg tgtcccaacg
    34501 caccaaacgt ttcggtaatt tgtagcgcaa ccagttacgc gttgaagcaa ggattcgaac
    34561 gtctttgcgg cttaatccta cttcttcaaa caattcacgg tacatcgcct gctctgcgga
    34621 ttctccgggg ttgattccgc cttgcggaaa ttgccaggag tgctgaccaa atcgccgggc
    34681 ccacattacc tgcccctggc gattacaaat cacgatacct acgtttgggc ggtagccatc
    34741 gtcatcaatc accggactac ctcaaaataa agctttatat acgaatgatt gtttcatact
    34801 ccaggaagac ggtaaaccac tctctgcagg gcattacaca ctaacaacaa ttgaataact
    34861 cacagttatg tgcagagtta taaacagagg aaggggtgga tagccgtttt tattcacctt
    34921 ttctgtggat agagttgtga agaactacgg aattactacg ggaaaacccg gagcattctg
    34981 aataagcccg atatagacat tttaaaatat tcattattag tcaatgcatt acatgtaatt
    35041 taagtaatga aaaagagtaa ttcgtgaccc aggtcacacc tctcatttac gggttggcga
    35101 aagatcaacc aatgccgtat ttatccacag aatgtgccac taagttaagc actgaaccac
    35161 taaaaactgg agtttcgtcg cacgtcaagg ctgtaaatgg aaacagtagt ggaggttttt
    35221 cacagttatc ccagctttct gtggataaca tggtgtaaga tcctgtttat tttcagtgac
    35281 cagatttgga aaacccgttg cagtgttgcg caactcgatt accggcaacc taaaaagcaa
    35341 tataaatcag ataattgaac aacgcatgtg gaaaaagtta cactgcgaat attcggcaca
    35401 taattgctgt ttgtttttta accaaggtat catgacatgt cccaacctcg cccactgctc
    35461 tctcctcccg aaactgaaga acagttgtta gcgcaagcac agcaactttc tggttataca
    35521 ttgggagaac tggcggcact tgccgggctg gttacgccag agaatttaaa acgcgataag
    35581 ggctggattg gcgtgttact ggagatctgg ctaggtgcca gcgcagggag taaacctgaa
    35641 caagattttg ctgctctggg cgtggaactt aaaactatcc ctgtggatag tcttggtcgt
    35701 ccgctggaaa caacattcgt ttgtgttgcc ccgttaacgg gcaatagcgg agtgacctgg
    35761 gaaaccagcc acgtgcgcca caaactcaaa cgcgtactgt ggataccggt tgaaggcgag
    35821 cgcagcatcc cgctggcgca gcgtcgcgtt ggatcaccgt tgctgtggag cccgaatgaa
    35881 gaggaagacc ggcagctacg cgaagactgg gaagaattaa tggatatgat tgttctcggt
    35941 caggttgagc ggattaccgc tcgtcacggg gagtatttac agatacgacc gaaagcagcg
    36001 aatgcgaaag cgcttaccga agccattggt gcacggggcg aacggattct gacgctgccg
    36061 cgcggctttt atttgaagaa gaatttcacc agtgcactac tggcccgtca ttttctgatc
    36121 cagtagccat cgctttgacc tgccgctttc cgggcatata attaccgctt catttttttg
    36181 gcagggcttt ttagatgtta tttgcatgga taaccgatcc taacgcctgg cttgcgctcg
    36241 gtacgctgac gctgctggag atcgttcttg ggatcgacaa tattattttc ctttctctgg
    36301 tggtggcaaa gctccccaca gcacaacgtg ctcatgcgcg ccgtctgggg ttggcgggag
    36361 ccatggttat gcgtctggcg ctgctggcat caatcgcctg ggttacgcgc ctgacgaatc
    36421 cgctttttac aatattcagt caggaaattt ctgcccgtga cttgattctg cttctgggtg
    36481 gcttgttcct tatctggaaa gccagcaagg aaatccacga atccattgaa ggtgaagaag
    36541 aagggctgaa aacacgcgtt tcatcattcc tcggcgctat cgtgcagatt atgctgctgg
    36601 atattatctt cagcctcgac tcggtgatta ccgctgtggg tctgtcagat cacctgttta
    36661 ttatgatggc cgccgtggtg attgccgtag gcgtgatgat gttcgccgcg cgctcgattg
    36721 gtgattttgt cgagcgccat ccttcggtaa aaatgctggc gctctctttc ctgattctgg
    36781 tgggctttac cctgattctg gaaagtttcg acatccacgt accgaaaggt tacatctact
    36841 tcgcgatgtt cttctctatt gcggttgaaa gcctcaacct gattcgcaac aaaaagaacc
    36901 cgctctgata ctccgtacgc tctcctgcgg gagggcgtac tcacttctgt taccctcaca
    36961 gtttaagatt ttcctgcttt caagatatat aacgtcagtt tataaacaga ctattatcat
    37021 aggtgggcca aatgaggata accggatgaa aaaatgggca gtaataattt ctgcagtcgg
    37081 actggcgttt gctgtttccg ggtgttccag tgattacgtc atggcgacca aagatggccg
    37141 tatgattttg accgatggaa aacctgaaat tgatgatgat accgggctgg tgagttatca
    37201 cgatcagcaa ggtaacgcga tgcaaattaa ccgtgatgat gtttcgcaaa taattgaacg
    37261 ttaacaaata aggtcagcat ccggctggcc ttaagatttt tctctttccc ttttcccttc
    37321 cctctgccat ttttatattc cttatgtcgt gattataaaa aggaaacggc tatgcaatat
    37381 caccgtatac cccacagttc gctggaagtc agcacgctgg ggcttggcac gatgacgttt
    37441 ggtgaacaga acagcgaagc cgacgcccac gcacaactcg actatgccgt cgctcagggc
    37501 attaacctta tcgacgttgc cgaaatgtac ccagtacctc cgcgccccga aacgcaaggg
    37561 ttaaccgaaa cctacgtcgg caactggctg gcgaaacatg gcagccgcga aaagttaatt
    37621 atcgcctcca aagtgagcgg accgtcgcgc aataatgaca agggcatccg cccggatcag
    37681 gcgctggatc ggaagaatat ccgcgaagcg ctgcatgaca gcctcaagcg tctgcagact
    37741 gattacctcg atctttatca ggtgcactgg ccgcagcgcc caaccaactg cttcggcaaa
    37801 ctcggttata gctggacaga ttctgcgcct gcggtttcgc tgctggatac gctggacgca
    37861 ctggcagagt accaacgagc gggaaaaatt cgttatatcg gcgtgtcgaa cgaaactgca
    37921 tttggcgtaa tgcgctacct gcatctggcg gacaaacacg atctgccgcg tattgtcacc
    37981 attcagaacc cttacagtct gttaaaccgc agttttgaag taggtctggc agaagtcagc
    38041 cagtatgaag gggtcgaact gctggcctat tcgtgcctgg gtttcggcac gctgaccggg
    38101 aaatatctca atggtgcaaa acccgctggc gcacgtaata cgctctttag tcggttcacc
    38161 cgctatagcg gtgagcaaac gcaaaaagcc gtcgcggcgt atgttgatat cgccagacgt
    38221 catggcctgg cccctgctca gatggcgctc gcgtttgtac gccgtcaacc gtttgttgcc
    38281 agcactctgc tgggcgcaac cacgatggat cagctgaaaa ctaacatcga aagtttgcat
    38341 ctggagttaa gcgaagacgt attagctgaa attgaagcgg tgcatcaggt ttatacttat
    38401 ccggcaccat aagattaacg ccggttaaaa ccggcgttaa atattaatga cggcgctgcc
    38461 agatccacag cgccgttatt gccagcgcaa atagcgcacc gaagccaatg ccaatgggca
    38521 cgaccgggat gcctaccatt accgccagcg agtaaatgcc cagcatcaac aacatggcgc
    38581 tgttttcgcc aaggttttgt actgcaatcg cattccccgc cccgacgctt tttttacccc
    38641 gctcctgtag caacgcattg agcggaacga caaaaaagcc ccccaacacg ccaatcagca
    38701 tcaacaaggc ataggctggc agctgctcgt gttgcaggga gaaaatcagt accaccacgc
    38761 caatcaaaat tccggctggc atacagcgtg acacggtttc cagcgtaacc agcttcgctg
    38821 ccgcacctgc gccaaccacg ataccaatcg ctaccatcgc gttgagatag gtcggcgtag
    38881 cgttatcggt aatgcccagc gccaccggta cccacagcac caacaggaaa cgcagcgtga
    38941 cacccgctcc ccagaataaa ctggtgccca ccagcgaaaa acgcgtttca ccattgcgcc
    39001 atagcgaggt gcaggcattc aggaaactgc gggtcatgtt gatgagattc caggactgcc
    39061 ccggacgcgc cgccgccagt ttgggaatgt agatattggc aacgaccgca ccaccgtagg
    39121 ccagtgcgca tgcggccagg gcgaccagga catgccagtc agccagcaca ccaccggcta
    39181 cggaaccgag caaaatcgcc gctatggtag aagcttccat taaaccgtta gcttttacta
    39241 acttactacc cgtggttaat tcgccgagaa taccgtattt cgccggtgaa taggctgcag
    39301 caccaacacc caccagcgta tagccgagaa acggattgat accaaagcag atactggctg
    39361 cgcccagcag cttcaggccg ttggcaaaca tcatcacccg gcctttggcg aagctatccg
    39421 ccacctgccc gacaaacggc gcaaaaagaa tgtaagcacc tacaaacacc atttgcagga
    39481 tgggctggct ccactccgga tagaactgcg ctttcagtaa cgccagagtg gcaaacagta
    39541 gggcattatc gccaaacgca gagagaaact gcgccacgat aaccgctttc atccccttcg
    39601 accacaacga agtgttagtg tgcactgact cactcatcgt gttgttccac ttcgtctacc
    39661 cagcttttca acgtgacaaa gtcaggtttg ccgctgccaa gtaatggcat ctgtttcaaa
    39721 tagcgaatat cacgcggtac agcaagctcc ggcacgccgt gctcacgggc atactgttgc
    39781 aacttatcgc gcgtcagttc gttatctgtg gtgaaaagca ccagtgcctc gcctttgctg
    39841 gcatcgctct taatcgcagt ggcatggact ttatctggcg aaacaccaag tgccagttgt
    39901 tccaccattt ccagcgacac catttcgcct gcaattttgg caaagcgttt tgcgcggccc
    39961 tgaatctgca caaagccctg ctcgtcaaaa cgcacaatat cgccagtgtc ataccagccg
    40021 cgctccattt cgccgcgaac attctcggcg gtgggcactt ccagtacacc tggcttctcc
    40081 acccgcagat agccgttcat tatgttcggc cctttcagtt gcaggcgtcc gccctcttcg
    40141 ataccaggga ccgacaacag gcgcgcatcc attcctggta gaatacgccc taccgtaccg
    40201 ggtttcgccg ccatcggtac gttgatagaa acgacaggcg cgcattcggt cacaccgtag
    40261 ccttcaagga tgcgcaggcc aaatttatcc tgccaaagct gtttggtact ttcttgtagt
    40321 ttttctgcgc ctgccaccac atagcgtaga cgatagaagt catacgggtt ggcgaaacgc
    40381 gcgtagtgac cgaggaaagt cgaagtgccg aacaacacgg tgcaactgcg gtcatacacc
    40441 aactccggca caatacggta atgcagcggg cttggataaa gaaacacttc tgcacctgtg
    40501 agcagtggcg taaacaggcc taccgtcagc ccaaaggagt gaaacagcgg taacgccgac
    40561 ataaagcgat cgttggtggt gaagtcggca atcgttttaa tctgctcgac attcgccaga
    40621 atgcttttat ggctatggac gacgcctttt ggatggcctt cagaaccgga ggtaaaaagg
    40681 atcagcgcct cttcttccgg ctgctgttta acctgtgcca gacgcggcat cagcaaatga
    40741 gcgaagatcc atactttgtc ggcagtggtg acatctgctt ttaaatcttc cagatagacc
    40801 cagcgcacct gagtaagttg ctccggcaga tgccagagtt tgcctttatc gagaaactgg
    40861 cgggaagtga agatggtttt gatttcagcc gccgtaatag cactggtcag cccttttacc
    40921 ccagcagtgt agttcatcat tgcgggaatg cggcgacggg cgatggcccc aaaaatcact
    40981 gccgcactga tgcctgcatt gggcagcatt aagccgatgc gttcgccttc aacactgtat
    41041 ttttcaagga tgcgtccaac aaacagcgtt ttcgtaagca atttgcgata ggagtctggg
    41101 gtaaaattga cgtcttcgac acatttcttc cctgctccga agcggtacat tgcactcagt
    41161 aaagattcgt acagcgtttc acgcgggcgc accgccattc gcgcttccat cattatttga
    41221 tgcagcattt cgccagcgat tttgcgacgg tcacgggcac gcggcgcatc cggcatctcc
    41281 acctgcgttg gtggcaaaat atgcagagta atttgcggga acaagcgacg tttaaccaga
    41341 cctttcaggc ggctgaagtg cgtaagttcc gccccttcaa tacgcacagg aataaccgtt
    41401 gcaccagact tcgccgcgac aaaacccgcg ccatcgtaga ttttcatcag cgagcctgtg
    41461 gtggtgatgc gtccttcagg gaaaatcacc actggtcggc cctgttccac cagacgtacc
    41521 agatgtttaa tcgccatagg ttgcgtcggg tcgagaggaa caaagtcgat aaatgatttc
    41581 agccaacgca tataccactg ttggcttatt gaggtgtaaa cggcaaacac tggacgcaca
    41641 ggtaaaaaca gtccaagcaa aatgccatca ataaaagaga cgtgattagg cgtaattaga
    41701 acgcgctcgc ccttcagtgc ctgggggtca cccgtaacgc gaacgcgata caaaacacgg
    41761 cacaaatttc gaaaaaagct aaaaagcatt ccaactccct ttgctctgat tcagtaaaag
    41821 cgaatggagg gagattacac gagataaaga acgcgagcga cagtaaatta ggtgcgaaaa
    41881 aaaacctgcg catccgcgca ggttggtgca agagacaggg tacgaagagc gtaccgaata
    41941 atctcaccaa tcaatacctc tgggatcttg attgtggtct gcacgacgac tcttcgccag
    42001 cgagaaaacg caaaggaatg aagggaaatg caacgaggtg tgtaaattgt cggttactgt
    42061 tacagattga tgaccggcaa aaaaaaacct gcgcatctgc gcaggctggt gtaattcatg
    42121 tgctcaaccc gaagttgact tcacctatca atacctctgg gatcaccact ttagcaacct
    42181 gaagccaaac gccaccagcg gtcaatcgca acagcgtttc gcaaagtgta accaaaggtt
    42241 tgcaatctct ttttttgtct tacttatttg cgtttggctc acattcccac gatgaaaaca
    42301 cgccgcccct tgaaccaacg ggcgttttcc gtaacactga aacaatgtaa gcgtttaccc
    42361 actaaggtat tttcatggcg accataaagg atgtagcccg actggcaggc gtttcagtcg
    42421 ccaccgtttc ccgcgtcatt aataattcac ccaaagccag cgaagcttcc cggctggctg
    42481 tgcatagtgc aatggagtct cttagctatc acccgaacgc caacgcccgt gcgctggcgc
    42541 agcagaccac tgaaacggtc ggtctggtcg ttggtgatgt ttccgatccg tttttcggtg
    42601 caatggtgaa agcggtcgaa caggtggctt atcacaccgg taatttctta ttgattggca
    42661 atggttacca caacgaacaa aaagagcgtc aggccattga gcaactgatc cgccatcgct
    42721 gtgctgcgtt ggtcgtccat gccaaaatga tcccggatgc cgatttagcc tcattattga
    42781 aacaaatgcc cggtatggtg ctgatcaacc gtatcctgcc tggctttgaa aaccgttgta
    42841 ttgctctgga cgatcgttac ggtgcctggc tggctacgcg tcatttaatt cagcaaggtc
    42901 atacccgcat tggttatctg tgctctaacc actctatttc tgacgccgaa gatcgtctgc
    42961 aagggtatta cgatgccctt gctgaaagtg gtattgcggc caatgaccgg ctggtgacat
    43021 ttggcgaacc agacgaaagc ggcggcgaac aggcaatgac cgagcttttg ggacgaggaa
    43081 gaaatttcac tgcggtagcc tgttataacg attcaatggc ggcgggtgcg atgggcgttc
    43141 tcaatgataa tggtattgat gtaccgggtg agatttcgtt aattggcttt gatgatgtgc
    43201 tggtgtcacg ctatgtgcgt ccgcgcctga ccaccgtgcg ttacccaatc gtgacgatgg
    43261 cgacccaggc tgccgaactg gctttggcgc tggcggataa tcgccctctc ccggaaatca
    43321 ctaatgtctt tagtccgacg ctggtacgtc gtcattcagt gtcaactccg tcgctggagg
    43381 caagtcatca tgcaaccagc gactaaccgc agttaaagca attccagcgc cagtaattct
    43441 tcgatggtct ggcgacggcg aatcaaccgc gcctgaccat tatcaaacag aacttctggt
    43501 aacagcggac ggctattgta gttggatgac attgatgcgc catatgcccc tgtatcatgc
    43561 agtaccagat aatcacctgc cttcacttcc ggcaaggcgc ggggttcaac atttccccct
    43621 tcctgctggg taaagacatc gcccgattca cataacggtc cggcgacgac ggtttccacc
    43681 gttggtgcgt gttccagaga acgaccatca gctgccaggg cactgatatg gtggtaacta
    43741 ccgtacattg ccgggcgcat cagatcgtta aacccggcat caaccagcac aaagtggcgg
    43801 ctacccattt gtttgacgct ccgcacctga gtaattaaca cgccagactg cgctaccagg
    43861 aagcgacccg gttcaatttc cagtttcaca gggtggccca aatggcgggc gatttgctca
    43921 cgcgcggcat tccacagacc ataataatgt tcggtatcaa ccgcctcttc accctgttga
    43981 taaggaatag aaagcccacc gcccgcagaa atagcctgta aatcctgacc gaattcgatg
    44041 acctgacgca ccatagcacc acacacctgt tccagatggg cataatcaac gccagaacca
    44101 atgtgcatgt gaatgccgac cagctgcaga tgatgacgtt gtatcacgtc cagtgcggcg
    44161 ggcagatcgg tgtaccagat accgtgcttg ctgttttcgc caccggtatt ggttttttgg
    44221 ctatgtccgt gaccaaaccc cggattaacg cgcagccata cccgatgccc tggcgaaacc
    44281 tggcccagtt ggtcgaacat atcaacagaa cccgcattca ccggaatttg caattcactg
    44341 acgcgttcaa gcgtcgcctg atcgataaca tctgccgtaa aaacaatatc atcggggtgc
    44401 gtttgcggat tgtaacccgc cgccaacgca cgctctattt cgcctaacga gacggaatcc
    44461 actttcacgc cctgctcacg cattaagcgc aaaatatgaa tattggaaca ggctttctgt
    44521 gcaaagcgca ccacatcaaa ctgtttcagc gctgcaatct gccgacgaat aatttgcgca
    44581 tcgtagaccc acaccgggca gccaaattca gcgggcaaac gcagcagatt ttcggcggtg
    44641 agatcggtat cggtgctgaa cagtgaatgt ggcataacaa actccagatg agtgcttttt
    44701 tatgattacg ccacatcata aaaagaataa aaaatatcga tttatgtcga gtctatgcaa
    44761 aaatgatatg gattaccgga ttgcgagaga gcgctaatgg ccgccgttaa cttacgtcat
    44821 attgaaattt ttcatgcggt aatgaccgcc ggaagcctga ctgaggcagc acacctgcta
    44881 cacacctcac agccaaccgt cagccgcgaa ctggcgcgct ttgagaaggt gatcgggctg
    44941 aaattgtttg agcgcgtacg tggacgatta catcctaccg tgcaaggact gcgtctgttt
    45001 gaagaagtgc aacgatcctg gtacggactg gatcgcattg tcagcgccgc agaaagtctg
    45061 cgcgagtttc gccagggaga actgtctatt gcctgcctgc cggtcttttc gcaatctttt
    45121 ttaccgcagc tcctgcaacc ctttctggta cgttatcccg atgtcagctt aaatatcgtg
    45181 ccccaggaat caccgctact tgaagagtgg ctctcggccc agcgtcatga tttaggactc
    45241 actgagacac tccatacgcc tgcgggaaca gaacgtaccg aattactctc tttagatgaa
    45301 ttgtgtgtgt tacctccggg tcatccgctg gcggtaaaaa aggtattaac gccggatgat
    45361 tttcagggtg agaactacat cagcctttcc cgtactgaca gctatcgcca gttgctggat
    45421 cagctattta ctgaacatca ggttaaacga cgcatgatcg tagaaaccca cagcgccgcg
    45481 tcagtctgcg caatggtacg ggcgggggta ggtgtttcgg tggttaaccc gctcaccgca
    45541 ctggattatg cggcaagcgg tttagtggtg cggcggttca gtattgcggt tccgttcacc
    45601 gtcagcctga tccgccccct gcaccgcccg tcatcagcgc tggtgcaggc gtttagtgag
    45661 catttacaag cggggttacc gaaactggtc acttctcttg acgctatttt gtcgtcagct
    45721 acgacagcat aaaagcgaca gcatcctcgg catggatcgc cgcagtatca aacacaggca
    45781 aaacactgcg ctcttctggc accagtaaac caatttctgt gcagccaaaa atgacgccct
    45841 gtgcgccctg ttctgcaagg cgagcaatca cttgcgcata ataagcgcgt gacgcttcgg
    45901 taaattgccc cagacacagt tcttcaaaaa taatctgatt aattttcgcc cgttcatccg
    45961 cttcaggaat aagacaattg atggaaaatt gttccgtcag ccgcccgcga taaaaatcct
    46021 gttccatggt gtaacgcgta cccagcagcg ccacacgagt cattccggcc ccggtaattg
    46081 cacgtccggt ggcatccgca atgtgtaaga aaggcagagt gcaacgtgac tcaatggcat
    46141 ccgccacttt atgcatcgta ttggtacata acacaatacc ttctgcgccc gcccgctgta
    46201 agccaagcgc cgcctcagcc agaatgtccc cggttttatc ccattccccg cgacgctggc
    46261 actcttctat ttcatgaaaa tcgacgctat gtagcagcac ttgcgcagag tgaagcccac
    46321 caagccgctg tttaatgcct tcatttatca gacgatagta aggaatggtg gattcccagc
    46381 tcattcctcc cagcaaacca attgttttca ttcctctctc ctgatgtgtg ttaccgcaac
    46441 aaggctgaat aaggaacgag acaaacgcct caacggccag gtgcccgatc tctattaacg
    46501 aaaaaagggc cggatgtaca gcacatccgg cccgtgaaat cagacgccga tatttctcaa
    46561 cttctcgcct gccatcagtt tgcgttcgat atgttccagc gtgacatttt tggtttccgg
    46621 aatgagccag aaagtgatgc ccacaaacgc aatgttcagc gcagtgtaga gccagaacgt
    46681 accggcagcg ccaatgctat caagcagtgt caggaaggtc gcgccgataa tcatattcga
    46741 cacccagttt gtcgtcgtcg aacaggtaat accgaaatcg cggcatttca gcggctgaat
    46801 ttcagagcac aggatccaca ccactggcgc ggcgctcatc gcataaccgg caatacacat
    46861 catcgtcatg ccaacagaga gccaggacaa gccactggaa gccgtaccgt tatcaaactg
    46921 catcaggcaa tagcccagca ccagagtgcc taacgccatc acgctgaaac caattttcag
    46981 agccggttta cgacctgctt tatctaccgt aaagaccgca atgaaggtcg cgaacataaa
    47041 ggtcagtccc acgaccagag tcgcaatcat ctgttgttct gtggtcgtaa agcccgccat
    47101 tttgaagata cgcggcgcat agtacatgat gatgttcata ccggtaaact gctgcatcgc
    47161 ctgcaacaac ataccgagaa acacagcacg acggacgtta cggttgatct taaacagtgc
    47221 ccaaccgcct tgttttaact tcaggctttc acgaatttcg ttgagttctt ctcgcgcttt
    47281 ttccgacgta tcgcgcagca tacgcaacac ttcttccgcc tcaatatgac gccccttttc
    47341 cgccagccag cgcgggctat ttggcaggaa gaccaccaga ataatcagca ggactgctgg
    47401 taaagcaaga acccccaaca ttgcgcgcca gttaccgcta taactgaacg ctgtatcgga
    47461 taaaaacgcc agcacgatgc cgagtgtgac catcaactgg tacatactga tcatcttacc
    47521 gcgaacgttt tcacttgcca tttcagaaag atacagagga gcggtgtaag acgcgatccc
    47581 gacagcaatg cccagcacca cacgagcggc gattaacatc tctacgctgg tcgcaaaagc
    47641 ggaccctata gaaccgagta caaacaggat ggcccccgcc atcaggctgt atttacgccc
    47701 caggcggaac gacagccaac cattaaacag cgcaccaatt gctgcgccga gcatcatgct
    47761 actaaccacc cattcctgca aacgactggt cagcacaaag tgatcggtaa tgaacggcaa
    47821 cgctccggcg attacgccga tatcaagacc aaataacaat cctgcgaccg cagcagctac
    47881 cgaaacaaac atattcatac gccgcgtatc acgcaaagaa cgtggcgtta aagcagattc
    47941 cgtattgata gtaaccattt tttcctgcca gcagagagta agacatagtg aaaaaatacg
    48001 tgaacaattc acacaggtgt caggtcggaa acagcataaa tatggattaa attgctgcga
    48061 catgccgtta tgtgatggat
//
LOCUS       DAZKIP010000021        46002 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZKIP010000021 DAZKIP010000000
VERSION     DAZKIP010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.021"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..2768)
                     /gene="rhsB"
                     /locus_tag="ACHOUK_001461"
     CDS             complement(<1..2768)
                     /gene="rhsB"
                     /locus_tag="ACHOUK_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HFP9896936.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQ
                     ALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGR
                     TQTFHREAAGEFSGEITGVTDGAGRHFRLVLTTQAQRAEEARQQAISGGTEPSAFPDT
                     LPGYTEYGRDNGIRLSAVWLTHDPEYPENLPAAPLVRYGWTPRGELAVVYDRSGKQVR
                     SFTYDDKYRGRMVAHRHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDRITITDS
                     LNRREVLHTQGEGGLKRVVKKEHADGSVTQSQFDAVGRLRAQTDAAGRTTEYSPDVVT
                     GITTPDGRASAFYYNHHSQLTSATGPDGLEIRREYDEWGRLIQETAPDGDITRYRYDN
                     PHSDLPCATEDATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQMTAVHREEGLS
                     QYRAYDSRGQLIAVKDTQGHETRYEYNAAGDLTTVIAPDGSRNGTQYDAWGKAICTTQ
                     GGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLTGKLI
                     RSEDEGLVTHWHYDEADRLTHRTVKGETAERWQYDERGWLTDISHISEGHRVTVHYGY
                     DEKGRLTGERQTVHHPQTEALLWQHETRHAYNAQGLANRCIPDSLPAVEWLTYGSGWL
                     AGMKLGDTPLVDFTRDRLHRKTLRRFGRYELTTAYTPAGQLQSQHLNSLQYDRDYTWN
                     DNGELIRISSPRQTRSYSYSDSGRLTGVHTTAANLDIRIPYATDPAGNRLPDPELHPD
                     STLSMWPD"
     gene            complement(2928..3140)
                     /gene="rpmE"
                     /locus_tag="ACHOUK_001462"
     CDS             complement(2928..3140)
                     /gene="rpmE"
                     /locus_tag="ACHOUK_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFP9896937.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            3343..5541
                     /gene="priA"
                     /locus_tag="ACHOUK_001463"
     CDS             3343..5541
                     /gene="priA"
                     /locus_tag="ACHOUK_001463"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFP9896938.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDSEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGHAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            5697..6722
                     /gene="cytR"
                     /locus_tag="ACHOUK_001464"
     CDS             5697..6722
                     /gene="cytR"
                     /locus_tag="ACHOUK_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFP9896939.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            6814..7773
                     /gene="ftsN"
                     /locus_tag="ACHOUK_001465"
     CDS             6814..7773
                     /gene="ftsN"
                     /locus_tag="ACHOUK_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFP9896940.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVTRAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            7866..8396
                     /gene="hslV"
                     /locus_tag="ACHOUK_001466"
     CDS             7866..8396
                     /gene="hslV"
                     /locus_tag="ACHOUK_001466"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFP9896941.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            8406..9737
                     /gene="hslU"
                     /locus_tag="ACHOUK_001467"
     CDS             8406..9737
                     /gene="hslU"
                     /locus_tag="ACHOUK_001467"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFP9896942.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            9804..10730
                     /gene="menA"
                     /locus_tag="ACHOUK_001468"
     CDS             9804..10730
                     /gene="menA"
                     /locus_tag="ACHOUK_001468"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFP9896943.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVVGIFLSQWAA"
     gene            10823..11308
                     /gene="rraA"
                     /locus_tag="ACHOUK_001469"
     CDS             10823..11308
                     /gene="rraA"
                     /locus_tag="ACHOUK_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFP9896944.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            11368..12042
                     /locus_tag="ACHOUK_001470"
     CDS             11368..12042
                     /locus_tag="ACHOUK_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9896945.1"
                     /translation="MQNIILLFIYLLLIAVNHFRYKFLLSGNAGEMILYFANVFFNPL
                     ALSFIIATIICITIKKRSSRSFIRGTCWLMGLFLIYSSYTVWERHSIWDYTFPEPGIT
                     VTVPSKQWVANIVKQGPTLVTRDAHAILAIVAFSQNELGVHSIEELTATQNGTAEMHV
                     CDVQGFACAYQEGVQTMSDGKVRHAIFMTLLDNTNLIQLVAAIDPDYLDEYQEEVMKM
                     MLSARQ"
     gene            12165..12791
                     /locus_tag="ACHOUK_001471"
     CDS             12165..12791
                     /locus_tag="ACHOUK_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9896946.1"
                     /translation="MVGEYIGSVLLGPLLLPVLVSGILCTFTKKTRNFASFTRGCCWV
                     LGVLLLSNVGNTFRLFTPWHYTFEKAAISVTVPNRHWNTVSISTDKTIDIRSEDNSVF
                     ISAFRLPAGRSADDSLEELKKMQRDNLKDQYNEETFQFHDCNAKHFTCKYQDVLINFD
                     GQQKRTISVYLEDTPRAVGIIALMEPDTADKYRQQAMEIMLSAKNTVK"
     gene            complement(12830..13075)
                     /gene="zapB"
                     /locus_tag="ACHOUK_001472"
     CDS             complement(12830..13075)
                     /gene="zapB"
                     /locus_tag="ACHOUK_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFP9896947.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            13501..14346
                     /gene="glpF"
                     /locus_tag="ACHOUK_001473"
     CDS             13501..14346
                     /gene="glpF"
                     /locus_tag="ACHOUK_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFP9896948.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            14369..15877
                     /gene="glpK"
                     /locus_tag="ACHOUK_001474"
     CDS             14369..15877
                     /gene="glpK"
                     /locus_tag="ACHOUK_001474"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFP9896949.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            16107..17117
                     /gene="glpX"
                     /locus_tag="ACHOUK_001475"
     CDS             16107..17117
                     /gene="glpX"
                     /locus_tag="ACHOUK_001475"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFP9896950.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANSLA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIESGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            17214..17960
                     /gene="fpr"
                     /locus_tag="ACHOUK_001476"
     CDS             17214..17960
                     /gene="fpr"
                     /locus_tag="ACHOUK_001476"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFP9896951.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVHPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(17965..18393)
                     /gene="uspD"
                     /locus_tag="ACHOUK_001477"
     CDS             complement(17965..18393)
                     /gene="uspD"
                     /locus_tag="ACHOUK_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFP9896952.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKLSADLLIVPFIDK"
     gene            complement(18420..18719)
                     /gene="yiiS"
                     /locus_tag="ACHOUK_001478"
     CDS             complement(18420..18719)
                     /gene="yiiS"
                     /locus_tag="ACHOUK_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFP9896953.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(18931..19371)
                     /gene="yiiR"
                     /locus_tag="ACHOUK_001479"
     CDS             complement(18931..19371)
                     /gene="yiiR"
                     /locus_tag="ACHOUK_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFP9896954.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            19472..20071
                     /gene="yiiQ"
                     /locus_tag="ACHOUK_001480"
     CDS             19472..20071
                     /gene="yiiQ"
                     /locus_tag="ACHOUK_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFP9896955.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            20179..20946
                     /gene="tpiA"
                     /locus_tag="ACHOUK_001481"
     CDS             20179..20946
                     /gene="tpiA"
                     /locus_tag="ACHOUK_001481"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFP9896956.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(21001..21756)
                     /gene="cdh"
                     /locus_tag="ACHOUK_001482"
     CDS             complement(21001..21756)
                     /gene="cdh"
                     /locus_tag="ACHOUK_001482"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFP9896957.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(21863..22852)
                     /gene="sbp"
                     /locus_tag="ACHOUK_001483"
     CDS             complement(21863..22852)
                     /gene="sbp"
                     /locus_tag="ACHOUK_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFP9896958.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKLGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLISWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(23171..24133)
                     /gene="pfkA"
                     /locus_tag="ACHOUK_001484"
     CDS             complement(23171..24133)
                     /gene="pfkA"
                     /locus_tag="ACHOUK_001484"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFP9896959.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(24314..25216)
                     /gene="fieF"
                     /locus_tag="ACHOUK_001485"
     CDS             complement(24314..25216)
                     /gene="fieF"
                     /locus_tag="ACHOUK_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFP9896960.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(25365..25865)
                     /gene="cpxP"
                     /locus_tag="ACHOUK_001486"
     CDS             complement(25365..25865)
                     /gene="cpxP"
                     /locus_tag="ACHOUK_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFP9896961.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            26015..26713
                     /gene="cpxR"
                     /locus_tag="ACHOUK_001487"
     CDS             26015..26713
                     /gene="cpxR"
                     /locus_tag="ACHOUK_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFP9896962.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            26710..28083
                     /gene="cpxA"
                     /locus_tag="ACHOUK_001488"
     CDS             26710..28083
                     /gene="cpxA"
                     /locus_tag="ACHOUK_001488"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFP9896963.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(28189..28863)
                     /gene="yiiM"
                     /locus_tag="ACHOUK_001489"
     CDS             complement(28189..28863)
                     /gene="yiiM"
                     /locus_tag="ACHOUK_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFP9896964.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYIGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(29012..29995)
                     /gene="kdgT"
                     /locus_tag="ACHOUK_001490"
     CDS             complement(29012..29995)
                     /gene="kdgT"
                     /locus_tag="ACHOUK_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFP9896965.1"
                     /translation="MQIKRLIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(30255..30875)
                     /gene="sodA"
                     /locus_tag="ACHOUK_001491"
     CDS             complement(30255..30875)
                     /gene="sodA"
                     /locus_tag="ACHOUK_001491"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFP9896966.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            31160..32194
                     /gene="rhaT"
                     /locus_tag="ACHOUK_001492"
                     /pseudo
     CDS             31160..32194
                     /gene="rhaT"
                     /locus_tag="ACHOUK_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(32191..33039)
                     /gene="rhaR"
                     /locus_tag="ACHOUK_001493"
     CDS             complement(32191..33039)
                     /gene="rhaR"
                     /locus_tag="ACHOUK_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFP9896967.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(33113..33949)
                     /gene="rhaS"
                     /locus_tag="ACHOUK_001494"
     CDS             complement(33113..33949)
                     /gene="rhaS"
                     /locus_tag="ACHOUK_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFP9896968.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYHCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            34237..35706
                     /gene="rhaB"
                     /locus_tag="ACHOUK_001495"
     CDS             34237..35706
                     /gene="rhaB"
                     /locus_tag="ACHOUK_001495"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFP9896969.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALISATQALPACRFIINP
                     NDDRFINPETMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVARIHSTRQTKELCA"
     gene            35703..36962
                     /gene="rhaA"
                     /locus_tag="ACHOUK_001496"
     CDS             35703..36962
                     /gene="rhaA"
                     /locus_tag="ACHOUK_001496"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFP9896970.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWIIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKETLSRRG"
     gene            37137..37961
                     /gene="rhaD"
                     /locus_tag="ACHOUK_001497"
     CDS             37137..37961
                     /gene="rhaD"
                     /locus_tag="ACHOUK_001497"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFP9896971.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAL
                     YHDNFHPQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAEVLVKVYSMGGMKQTISREELIALGQRFGVTPLASALAL"
     gene            37971..38285
                     /gene="rhaM"
                     /locus_tag="ACHOUK_001498"
     CDS             37971..38285
                     /gene="rhaM"
                     /locus_tag="ACHOUK_001498"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFP9896972.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTEICQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            complement(38326..39720)
                     /locus_tag="ACHOUK_001499"
     CDS             complement(38326..39720)
                     /locus_tag="ACHOUK_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFP9896973.1"
                     /translation="MKKSTLSLAIGLLLACSTGMAKTQHLMLEQRMALLEERLEAAEM
                     RAAKAESQVKQLQTQQAAEIREIKAAQGNTPVNGQATAESAKKNATSPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKMDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKENDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMMKEGRGRSNAGGNFLVSKQLDNWYFELNTLLEDGTSLYNDGNYHGRD
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPDNGIVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSG
                     VVCDNDCWIDDEGTYTIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKKHGDDT
                     DDRYGARVRFKYFF"
     gene            40198..40644
                     /gene="frvA"
                     /locus_tag="ACHOUK_001500"
     CDS             40198..40644
                     /gene="frvA"
                     /locus_tag="ACHOUK_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFP9896974.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATMALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSTCVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            40655..42094
                     /gene="frvB"
                     /locus_tag="ACHOUK_001501"
     CDS             40655..42094
                     /gene="frvB"
                     /locus_tag="ACHOUK_001501"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFP9896975.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAIPYAL
                     AAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLAIGLA
                     LNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            42084..43154
                     /gene="frvX"
                     /locus_tag="ACHOUK_001502"
     CDS             42084..43154
                     /gene="frvX"
                     /locus_tag="ACHOUK_001502"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFP9896976.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSREEAKKRGAEIGDFISPEAN
                     FACWGEDKIVGKALDNRIGCAMMAELLQTVNNPGITLYGVGSVEEEVGLRGAQTSAEH
                     TKPDVVIVLDTAVAGDVPGIDNIKYPLKLGNGPGLMLFDKRYLPNQKLVAALKNCAAH
                     NGLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            43154..44902
                     /gene="frvR"
                     /locus_tag="ACHOUK_001503"
     CDS             43154..44902
                     /gene="frvR"
                     /locus_tag="ACHOUK_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFP9896977.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EHNGLFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGLASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(44952..>46002)
                     /gene="yiiG"
                     /locus_tag="ACHOUK_001504"
     CDS             complement(44952..>46002)
                     /gene="yiiG"
                     /locus_tag="ACHOUK_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFP9896978.1"
                     /translation="RNLLSSAIIVAIMALGLTGCDDKKAETETPLPANSQPAAPAPEA
                     KPTEAPVAKADAKPETPAQPLVDEQAVFDEKMDVYIKCYNKLQIPVQRSLARYADWLK
                     DFKQGPTGKESTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVAL
                     GNTINEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKRQL
                     AELKNIEEREGKTFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKE
                     ADKGGMNFSFINSADQYQLETKKYVRRVRDKVPYSDWDKEHLQDANTSWMVEDSFPRA
                     LREYNEMVDDYNSLR"
BASE COUNT        11090 a        11935 c        11826 g        11151 t
ORIGIN      
        1 ttatccggcc acatgctgag ggtgctgtcc gggtgcagct ccgggtcggg caggcggtta
       61 cctgccgggt ccgtggcata cgggatgcgg atatccagat tcgctgcggt ggtgtgaacg
      121 ccggtcagcc tgccggagtc gctgtagctg taactccggg tctggcgcgg gctgctgatg
      181 cggatgagtt cgccgttgtc gttccaggtg taatcgcggt catactgcag gctgttcagg
      241 tgctggctct gtaactgccc ggcaggggta taagcggtgg tgagttcata acggccgaat
      301 ctgcgcagcg ttttccggtg caggcggtcg cgggtgaaat ccaccagcgg tgtgtcgccg
      361 agcttcatgc ctgccagcca gccgctgcca taggtcagcc attccacggc gggcaggctg
      421 tccggtatac agcggttcgc cagcccctgc gcgttgtaag cgtgtctggt ctcatgctgc
      481 cagagcagtg cttccgtctg cgggtgatgc accgtctgac gctcaccggt cagccggcct
      541 ttctcatcat acccgtaatg caccgtcacc cggtgccctt cgctgatatg gctgatgtct
      601 gtcagccagc cgcgttcgtc atactgccag cgctctgcgg tttcaccctt cacggtgcgg
      661 tgcgtgaggc ggtctgcttc gtcatagtgc cagtgggtga ccagcccctc atcctcgctg
      721 cggataagtt tgccggtcag gtcgtggtga taacgctgtg tgcggccgtc aaagccggtt
      781 tcctgtatca gccggtcgag tacatcgtaa cggaaggtgg tgtggctgcc gttttcactg
      841 gtcaggcgga tgacccgtcc ggcagcatcg tattccatac tgcgcgtcag accgccctgc
      901 gtggtacaga tggcttttcc ccacgcatcg tactgtgtcc cgtttctgct gccgtccggg
      961 gcaatgacgg tggtcaggtc accggcggcg ttgtattcat accgcgtttc atggccctgc
     1021 gtgtctttca cggcaattaa ctgtccacgg ctgtcgtatg cgcggtactg actcagccct
     1081 tcctcgcggt gcaccgccgt catctgcccg aagcggtcat ggtcataacg ggttacataa
     1141 ccggaacagt cggtgaagct cagcaactga ccgtaacggc tccacgtcat ggttttccgg
     1201 ctgccggtgg catcttccgt tgcgcagggt aagtcactgt gtggattatc ataacggtag
     1261 cgggtgatat cgccgtcagg ggcagtttcc tgaatcagac ggccccattc atcatattcc
     1321 cggcgtattt ccagcccgtc aggcccggtg gctgacgtta actggctgtg gtggttatag
     1381 taaaacgccg atgccctgcc atccggcgtg gtgatgcccg tcaccacatc cgggctgtat
     1441 tctgttgtcc tgcctgcggc atccgtctgt gccctgagcc tgcccaccgc gtcaaactga
     1501 ctctgcgtga cgctgccgtc cgcgtgttcc tttttcacca cccgcttcag cccgccttca
     1561 ccctgcgtgt gcaggacttc acggcggttc aggctgtcgg tgatggtgat gcggtctttc
     1621 tcatactgat acgtgtagct taagcctgcc gggtttagct gttctgtcac ccgcccgtcg
     1681 ctgtcgtaac ggtaacggat ttccggccgg cccgtgtgac ggtgcgccac catccggccc
     1741 cggtatttat catcgtaagt aaagctgcgc acctgtttgc cactacggtc atacaccacc
     1801 gccagttcgc cgcgcggcgt ccagccatag cgcaccagcg gcgcggcagg taaattctcc
     1861 gggtattccg ggtcgtgcgt cagccacacg gcagacagac ggatgccgtt gtcccggcca
     1921 tattcggtgt aacccggcag ggtatcagga aaagcggacg gttccgtccc gccggaaatg
     1981 gcctgctgcc gggcttcttc tgcccgctgc gcctgcgtgg tcagtaccag ccggaagtga
     2041 cgcccggcac catccgtcac gccggtgatt tcgccgctga attcaccggc ggcttcgcgg
     2101 tggaacgtct gtgtgcgccc gaagcggtcc accagcccgg tcagtacccg gtacggcggc
     2161 agcggcgcag gcagcacctc atccgcttcc ggcacccgct cacaccagcc gagcagccac
     2221 cacggcccct gcggactgtt tgtcgccaga taacgatgcg gacttaagcg gagttcttcc
     2281 ggcagcgcct gccagagtgc ggccagccgg tgaccttcat ccagtttcgc cacgccgccg
     2341 cgcaccagcc agagtgactc gctgcggctg taaccgtcct caccgggaaa caggtgctca
     2401 aaatacaggc ttctgccgcc gttatcactg agtatcagtg tgttatcgcg cagctgtaag
     2461 cggatatccg caggcatttt ccagccgggg ccgaggctcc ccaccggcgc gggcgttttt
     2521 gtccggtaac tgctgtaggt gcgggagagg atgaacggca gcgggccggg cagggcgatg
     2581 tcggtttcac cgggaaggac ctttgcaccg agcaggggat tgaccggatg gccggacgtc
     2641 actccgccgg ggcacaccga acaggccacg ccggtggggg caccaatgcg caccccggct
     2701 gaaccctgaa caatgctacc gccatactgc gtcatgtcgc cctgacgcgc cgccggtttt
     2761 ccgctcatcg taaaatcctt ttgaaataaa taagaaccag aagcctagcg ccagtcaaca
     2821 acaggaaatg ataatgagtt aacaccccgg aatttttcag aatttataaa aggcaaatta
     2881 caggcacaaa aaaagcgccg tgcggcgctt ttttcggaaa tccggtctta tttgctgccc
     2941 gggatgttga aacgcttgtt gaagcggtca acacggccac cggtagcaac atcacgctgt
     3001 ttgccagtga agaacgggtg gcacttgctg cacacgtcga ggttcaggtc atgaccaacg
     3061 gtggagcgga ttttcattac gttaccgcaa gagcagctag cagtaatttc ttcgtatttc
     3121 gggtgaatat cttttttcat gggaaaacct cggtttaagg ccgcgtcgct cttccagccc
     3181 taacgccaga caccacgcga tgttaaaagt atagcttcaa tacgatcatt tcgtacgaag
     3241 gcgcgaaatc atacagaaat taaccagcat atgcaaactg atccgcactc ttctacggca
     3301 atgtgtatac taacccaccg aatttcaagt caggatgatg ctatgcccgt tgcccacgtt
     3361 gccttgcccg ttccgcttcc tcgtaccttt gactatctcc tgccagaagg catggcggtt
     3421 aaagccgggt gtcgcgtgcg cgtgccgttt ggcaaacagc aggagcgcat cggggttgtg
     3481 gtgtcagtta gcgacgtcag tgagctgccg cttaacgagc taaaagcggt agtcgaagtg
     3541 ctggatagtg agccggtgtt tactcactcc gtctggcgat tgctgctctg ggcggcggat
     3601 tactatcatc atccgattgg cgatgtgctg tttcatgcct tgccgatttt actgcgccag
     3661 gggcggcctg cggcgaacgc gccgatgtgg tactggtttg ccactgaaca aggccatgcg
     3721 gtggatctga acagcctgaa acgttcgcca aagcaacaac aggcactggc ggcattgcgg
     3781 caaggcaaaa tctggcgcga ccaggttgcc acgcttgaat ttaatgatgc cgcgttacag
     3841 gcgctgcgca aaaaaggtct gtgtgattta gcaagtgaaa caccagagtt tagcgactgg
     3901 cgaacgaact atgccgtttc tggtgaacgg ttgcgattga ataccgaaca ggccaccgcc
     3961 gttggcgcaa ttcatagcgc ggcggatacc ttctctgctt ggctgctggc gggcgttaca
     4021 ggttccggta aaacagaggt ttatttgagc gtactggaaa acgtgctcgc tcagggcaaa
     4081 caggcgctgg tgatggtgcc ggaaatcggc ctgacaccgc aaactatcgc ccgttttcgt
     4141 gaacgtttta atgcccccgt agaagttctg cattccggcc tgaacgacag cgagcgtctt
     4201 tcggcgtggt tgaaagcgaa aaatggtgag gcggcgattg tcatcggcac ccgctccgcg
     4261 ctgtttacgc cgtttaaaaa tctcggtgtg attgtcattg atgaagagca cgacagttcc
     4321 tacaagcagc aggaaggctg gcgctatcat gcccgcgacc tggcggttta tcgtgcgcac
     4381 agcgagcaaa tcccgattat tcttggctcc gcaacacccg cactggaaac gttatgcaac
     4441 gtccagcaga aaaaataccg cctgctgcgc ctgacgcgtc gggcggggaa tgcgcgtccg
     4501 gcaattcaac atgtgctgga tttaaaaggt cagaaggtgc aggcggggct ggctccggcg
     4561 ttaatcaccc gtatgcgcca gcatttacag gccgacaacc aggttatcct ctttcttaac
     4621 cgccgtggct ttgcgcctgc cttgctgtgc cacgactgcg gctggattgc cgaatgtcca
     4681 cgttgcgatc actactacac gctgcatcag gcgcaacagc atttgcgctg tcaccactgt
     4741 gacagccaac gcccggtgcc gcgccagtgc ccttcctgcg gttccacgca cctggtaccc
     4801 gtggggctgg gcaccgaaca gcttgaacag acgctcgcgc cgttattccc tggtgtgccc
     4861 atttctcgta tcgaccgcga taccaccagc cgcaaaggag cgctggaaca acaactggcg
     4921 gaagtgcatc gcggcggcgc gaggatattg attggcacgc aaatgctggc gaaaggtcac
     4981 cactttccgg atgtgacgct ggtagcgtta ctggatgtgg acggcgcgct gttttctgcc
     5041 gattttcgct cggcagagcg ttttgcccag ctatacaccc aggtagcagg ccgtgccggg
     5101 cgtgcgggta aacagggcga agtggtgctg caaacgcatc atccggaaca cccgctattg
     5161 caaacgttgc tctataaagg ctacgacgct tttgccgaac aggcactggc tgagcggcga
     5221 atgatgcaac tgccgccgtg gaccagccat gtgattgtgc gtgcggaaga tcataacaat
     5281 cagcacgcgc cattgttcct gcaacaactg cgtaatctga tcctctccag cccgctggca
     5341 gacgacaaat tgtgggttct cggtccggtt ccggcactgg cacctaaacg tggcggtcgc
     5401 tggcgctggc agatattgtt gcagcaccct tcccgcgtgc gcttgcaaca catcattaac
     5461 ggtacgctgg cactcatcaa tacaataccg gattcccgta aggtgaaatg ggtgctggat
     5521 gttgatccga ttgagggtta aaccgctcac gatgcgaggc ggatcgaaaa attcaatatt
     5581 catcacactt ttcatgaaaa ttctgtaacc gttttcacgc gctatctgct aaaaatgttg
     5641 ccgatgtgaa gtaaacatgg atgtagtacg cctgacgtgc caggcgagga gtgagtgtga
     5701 aagcgaagaa gcaggaaact gccgcgacca tgaaagacgt tgccctcaag gcaaaagtct
     5761 ctacagcgac cgtctcccga gcattaatga atcccgataa agtctcccaa gccacccgta
     5821 atcgggttga aaaagcggcc cgtgaagtgg gttatttacc gcagcctatg gggcgcaacg
     5881 tcaagcgtaa tgaatcccgc accattctgg tgattgtccc ggatatctgc gatcccttct
     5941 ttagcgaaat tattcgcggt atcgaagtta cggcggcaaa tcacggatat ctggtgctga
     6001 ttggcgactg tgcgcatcaa aatcagcagg aaaaaacctt tatcgatttg atcatcacca
     6061 agcaaattga tggcatgttg ctgctgggtt caaggctgcc gtttgatgcc agcattgagg
     6121 aacagcgtaa tctgccgccg atggtgatgg cgaacgaatt tgcaccggaa ctggagctgc
     6181 ctaccgttca tatcgacaat ctgaccgccg catttgatgc agtaaattat ttatatgagc
     6241 aagggcataa acggattggc tgtatagccg gtcccgaaga gatgccgctg tgtcactacc
     6301 gcctgcaagg ttacgttcag gcgctgcgtc gctgcggcat tatggttgat ccgcaataca
     6361 tcgcccgtgg cgacttcacc ttcgaagccg gaagcaaagc gatgcagcag ctgcttgatc
     6421 ttccacaacc gcctactgct gtcttctgcc atagcgatgt gatggcgctc ggcgcacttt
     6481 ctcaggcaaa acgccagggg ctgaaagtcc cggaagacct ttccataatc ggttttgata
     6541 acatcgacct gacgcaattt tgtgatccgc cgctgacaac catcgcgcag ccgcgttacg
     6601 aaatcggtcg ggaagctatg ctgttattgc ttgatcaaat gcaggggcaa cacgttggca
     6661 gtggctctcg tttaatggac tgcgaactta tcatccgggg atcaacacgc gcgttacctt
     6721 aaagtaaacc tttaagactg ccgtgactgg tcaaaggccc gccgcttaag taacatggcg
     6781 ggctgacgaa cgaataaata cagcgaaacg atagtggcac aacgagatta tgtacgccgc
     6841 agccaaccgg caccttcgcg gcgaaaaaag agcacctcac ggaaaaagca acgaaatctg
     6901 cctgcggttt ctcccgctat ggtcgctatt gctgccgccg ttcttgtgac ctttatcggt
     6961 ggtctgtact tcattacgca tcacaagaaa gaagagtccg agacgctgca aagccagaaa
     7021 gtgaccggaa acggactacc accaaaacca gaagaacgct ggcgctacat caaagagctg
     7081 gaaagtcgcc agccgggtgt gcgtgcgccc acagaacctt ctgccggtgg tgaagtgaaa
     7141 acgccggagc aactgacacc agaacaacgt cagcttcttg aacaaatgca ggctgatatg
     7201 cgccagcagc caacgcagct ggttgaagtt ccgtggaatg aacagacgcc ggaacagcgt
     7261 cagcaaacgc tacagcgcca acgtcaggcg cagcaattag cggaacagca acgcctggct
     7321 cagcagtcgc gtacgactga acaaagctgg cagcagcaga cgcgtacgtc gcaagccgcg
     7381 ccggtacagg cgcaaccgcg ccagtctaaa ccggcttcta ctcagcagcc gtaccaggat
     7441 ctgctgcaaa cccctgcgca cacgaccgcg cagtcgaagc cgcagcaagc tgcgccggtt
     7501 actcgtgccg ctgacgcgcc aaaaccgacg gcggagaaaa aagacgaacg ccgctggatg
     7561 gtgcagtgcg gttcgttcag aggcgcggaa caggcagaga cggtacgtgc tcagctggcg
     7621 ttcgaaggct ttgactcaaa aattaccacc aacaatggct ggaatcgtgt ggtcattggc
     7681 ccggtgaaag gcaaagagaa cgcagacagc accctcaatc ggttgaagat ggcgggtcat
     7741 acaaactgca ttcggctcgc cgccgggggt tgaaaccctc aaaatccccc ccatctataa
     7801 ttgcattatg ccccgtactt ttgtacgggg tttgtactct gtattcgtaa ccaaggggtc
     7861 agctcgtgac aactatagta agcgtacgcc gtaacggcca tgtggtcatc gctggtgatg
     7921 gtcaggccac gttgggcaat accgtaatga aaggcaacgt gaaaaaggtc cgccgtctgt
     7981 acaacgacaa agtcatcgcg ggctttgcgg gcggtactgc ggatgctttt acgctgttcg
     8041 aactgtttga acgtaaactg gaaatgcatc agggccatct ggtcaaagcc gccgttgagc
     8101 tggcaaaaga ctggcgtacc gatcgcatgt tgcgcaaact tgaagcactg ctggcagtcg
     8161 cggatgaaac tgcatcgctt atcatcaccg gtaacggtga cgtggtgcag ccagaaaacg
     8221 atcttattgc tatcggctcc ggcggccctt acgcccaggc tgcggcgcgt gcactgttag
     8281 aaaatactga acttagtgct cgtgaaattg ctgaaaaggc gttggatatt gcaggcgaca
     8341 tttgcatcta taccaaccat ttccacacca tcgaagaatt aagctacaaa gcgtaaggat
     8401 ctcccatgtc tgaaatgacc ccacgcgaaa tcgtcagcga actggataag cacatcatcg
     8461 gccaggacaa cgccaagcgt tccgtggcga ttgctctgcg taaccgctgg cgtcgcatgc
     8521 agctcaacga agagctgcgc catgaagtga ccccgaaaaa tatcctgatg atcggcccga
     8581 ccggtgtcgg taaaactgaa atcgcccgtc gtctggctaa gctggcgaat gcgccgttca
     8641 tcaaagttga agcgaccaaa ttcaccgaag tgggctacgt cggtaaggaa gtggattcta
     8701 ttattcgcga tctgaccgat gccgccgtga aaatggtacg cgtccaggct atcgagaaaa
     8761 accgttatcg cgctgaagaa ctggcagaag aacgtattct cgacgtgctg atcccacctg
     8821 ctaaaaacaa ctggggacag accgaacagc agcaggaacc gtccgctgct cgtcaggcat
     8881 tccgcaaaaa actgcgtgaa ggccagcttg atgacaaaga aatcgagatc gatcttgccg
     8941 cagcaccgat gggcgttgaa attatggctc ctccaggcat ggaagagatg accagccagc
     9001 tgcagtccat gttccagaac ctgggcggtc agaagcaaaa agcgcgtaag ctgaaaatca
     9061 aagacgccat gaagctgctg attgaagaag aagcggcgaa actggtgaac ccggaagagc
     9121 tgaagcaaga cgctatcgac gctgttgagc agcacgggat cgtgtttatc gacgaaatcg
     9181 acaaaatctg taagcgcggc gagtcttccg gtccggatgt ttctcgtgaa ggcgttcagc
     9241 gagacctgct gccgctggta gaaggttgca ccgtttccac caaacacggg atggtcaaaa
     9301 ctgaccacat tctgtttatc gcttctggcg cgttccagat tgcgaaaccg tctgacctga
     9361 tcccggaact gcaaggtcga ctgccaatcc gcgttgaact gcaggcactg accaccagcg
     9421 acttcgagcg tattctgacc gagccgaatg cctctatcac cgtgcagtac aaagcactga
     9481 tggcgactga aggcgtaaat atcgagttta ccgactccgg tattaaacgc atcgcggaag
     9541 cggcatggca ggtgaacgaa tctaccgaaa acatcggtgc tcgtcgttta cacactgttc
     9601 tggagcgttt aatggaagag atttcctacg acgccagcga tttaagcggt caaaatatca
     9661 ctattgacgc agattatgtg agcaaacatc tggatgcgtt ggtggcagat gaagatctga
     9721 gccgttttat cctataatcg cgttcaatca ttttcatcat tgtttgatgg ggctgaaagg
     9781 ccccattttt attggcgcgt attatgactg aacaacaaat tagccgaact caggcgtggc
     9841 tggaaagttt acgacctaaa accctccccc tcgcctttgc tgcaattatc gtcgggacgg
     9901 cgctggcatg gtggcaaggt cattttgatc cgctggtcgc cctgctggca ctgattaccg
     9961 ccgggctatt acagatcctt tctaacctcg ccaatgatta cggcgatgcg gtaaaaggca
    10021 gcgataaacc tgaccgtatc ggaccgctac gcggcatgca aaaaggggtc attacccagc
    10081 aagagatgaa acgggcgctc attattaccg tcgtgctcat ctgtctctcc gggctggcac
    10141 tggttgcagt ggcgtgccat acgctggccg attttgtcgg tttcctgatt cttggcgggt
    10201 tgtcgatcat tgccgctatc acctacaccg tgggcaatcg tccttatggt tatatcggtc
    10261 tgggtgatat ttccgtactg gttttctttg gctggttaag cgtcatgggg agctggtatt
    10321 tacaggctca tacattgatt ccggcactga tccttccggc gaccgcatgc ggcctgctgg
    10381 caacggcagt actgaacatt aataacctgc gtgatatcaa tagcgaccgc gaaaacggca
    10441 aaaacacgct ggtggtgcgc ttaggtgaag tgaacgcgcg tcgttatcat gcctgcctgc
    10501 tgatgggctc tctggtatgt ctggcgctgt ttaatctctt ttcgctgcat agcctgtggg
    10561 gctggctgtt cctgctggcg gcaccgttgc tggtgaagca agcccgttat gtgatgcggg
    10621 aaatggaccc ggtggcgatg cgaccgatgc tggaacgcac tgtcaaggga gcgttactga
    10681 ctaacctgct gtttgttgta gggatattcc taagccagtg ggcagcataa ctgacaaata
    10741 tcaattaaca attgatgatt ttgccaacag accacatagc gcgatatact gaaaattctc
    10801 gcagcaactg aatgttaagc ctatgaaata cgatacttcc gagctttgtg acatctatca
    10861 agaagatgtt aacgtcgtgg aaccgctgtt ctccaacttt ggcggacggg cgtcgtttgg
    10921 cggacaaata atcacggtaa aatgtttcga ggacaacggg ttgctgtacg atctgctcga
    10981 acagaatggc cgtggtcgtg ttcttgtcgt cgatggcggt ggttctgttc gtcgcgcact
    11041 ggtcgatgct gaactggcgc gtctggcagt acaaaatgaa tgggaaggtc tggtcattta
    11101 cggcgcggtg cgtcaggtag atgacctgga agagttggat atcggcatcc aggcgatggc
    11161 ggcaattccg gttggtgccg ctggcgaagg cattggcgaa agcgatgtcc gcgtcaattt
    11221 tggcggtgtt accttcttct ccggcgacca tctttatgcc gacaataccg ggattattct
    11281 ttcagaagat ccgctggata ttgaataagt atctgccatg cgttaaaagg ggaattaaat
    11341 tccccttttt tatacatgga cgtattcatg cagaatatta ttcttttatt tatatattta
    11401 ttacttatag ccgttaacca tttccgctac aaatttcttt tatctggcaa cgcgggtgaa
    11461 atgattttat attttgctaa cgtatttttt aacccacttg ccttatcatt tattatcgcc
    11521 acaatcatct gtataacgat aaaaaaaaga agcagccgca gctttattcg cggcacttgt
    11581 tggttaatgg gattatttct tatctatagc agctatacgg tgtgggaacg acacagcata
    11641 tgggattata ctttcccgga gccaggtatc accgtcacgg ttccgagcaa acagtgggtt
    11701 gccaatatag taaaacaagg accaaccctg gtgacccggg atgcccatgc tattttagct
    11761 attgttgcct tctcacaaaa tgaactcggt gtgcattcaa tcgaggaact taccgcgact
    11821 caaaacggaa ctgcagaaat gcatgtttgt gatgttcagg gatttgcctg cgcttatcag
    11881 gaaggagtgc aaacaatgag tgatggcaaa gtaagacatg ctattttcat gactctactg
    11941 gataacacta atttgataca actggtcgca gctatcgacc cagattattt ggacgaatat
    12001 caagaagaag taatgaaaat gatgctttct gctcgtcaat gatctgatgt gcatgttaat
    12061 aataatggat tgatatgaga tacgttatct tattaatact atggataata actgtttttc
    12121 ttagtcatct tcagcacagt aacttgctta ctggagatcc agggatggtt ggcgagtata
    12181 tcggttcagt acttcttgga ccgttattac ttcccgtgct tgtgtcaggt attctgtgca
    12241 cattcactaa aaaaacgcgt aacttcgcca gttttactcg cgggtgctgt tgggtattgg
    12301 gagtcctgct tttatccaac gtcggcaata cgtttcgtct gttcaccccg tggcactata
    12361 cgtttgagaa ggcagctatt tccgtgactg tcccaaaccg acactggaat acggtttcta
    12421 ttagtactga taaaaccatt gatataagaa gtgaagataa tagcgtcttt atttctgctt
    12481 tccgcctgcc cgcaggacgt agcgccgatg actcgctgga agagttaaag aagatgcagc
    12541 gagacaatct caaggatcaa tacaatgaag aaaccttcca gtttcatgat tgtaatgcca
    12601 aacatttcac ctgtaagtat caggatgtgt tgataaattt tgatggtcag cagaaaagaa
    12661 ccatcagtgt ttatcttgaa gacaccccca gggctgtggg gatcattgca ttaatggagc
    12721 cagatacggc agataaatat cgccaacagg cgatggagat tatgttgtct gctaaaaata
    12781 cagtgaagta aaaaataagg cgccttgcgg cgcctctgcg tggaagagat taaacctctt
    12841 ccatgcgacc cagcagggcc tgcagacgtt cctgccagcc gttctgctgt tctttcagat
    12901 ggttgttctc acgctccagc tcttcgcgct gatgctgggc attttgaact tcctgcgaca
    12961 atgagttgtt tttttctttc agctcttcga tttccatctg caacagagtg atggtatcaa
    13021 tcgcctgctg tacttttgct tccagtttct caaacacttc taatgacatt gtcatacctc
    13081 tcctgaattg caaggcgttg atggataaaa aatcctcgtc ccgattaccg gtgacgcctt
    13141 aataaatacg agcgcacttt agttagctcc gattgtatga agccgcgcca tcgctgtcca
    13201 gcggcacgcc ttgcagatta cggtttgcca cacttttcat ccttctcctg gtgacataat
    13261 ccacaccaat cgaaaatgtt aataaatttg ttgcgcgaat gttctaacaa acatgcatca
    13321 tgtacaatca gatggaataa atggcgcgat aacgctcatt ttatgacgaa gcacacacat
    13381 tttaagttcg atatttctcg tttttgctcg ttaacgataa gtttacagca tgcctacaag
    13441 catcgtggag gtccgtgact ttcacgcata caacaaacat taactcttca ggatccgatt
    13501 atgagtcaaa catcaacctt gaaaggccag tgcattgctg aattcctcgg taccgggttg
    13561 ttgatttttt tcggtgtggg ttgcgttgca gcactaaaag tcgctggtgc gtcttttggt
    13621 cagtgggaaa tcagtgtcat ttggggactg ggggtggcaa tggccatcta cctgaccgca
    13681 ggggtttccg gcgcgcatct taatcccgct gttaccattg cattgtggct gtttgcctgt
    13741 ttcgacaagc gcaaagttat tccttttatc gtttcacaag ttgccggcgc tttctgtgct
    13801 gcggctttag tttacgggct ttactacaat ttatttttcg acttcgagca gactcatcac
    13861 attgttcgcg gcagcgttga aagtgttgat ctggcaggca ctttctctac ttaccctaat
    13921 cctcatatca attttgtgca ggctttcgca gttgagatgg tgattaccgc tattctgatg
    13981 gggctgatcc tggcgttaac ggacgatggc aacggtgtac cacgcggccc tttggctccc
    14041 ttgctgattg gtctactgat tgcggtcatt ggcgcatcta tgggcccatt gacgggtttt
    14101 gccatgaacc cagcgcgtga cttcggtccg aaagtctttg cctggctggc gggctggggc
    14161 aatgtcgcct ttaccggcgg cagagacatt ccttacttcc tggtgccgct ttttggccct
    14221 atcgttggcg cgattgtagg tgcatttgcc taccgcaaac tgattggtcg ccatttgcct
    14281 tgcgatatct gtgttgtgga agaaaaggag accacaactc cttcagaaca aaaagcttcg
    14341 ctgtaatatg actacgggac aattaaacat gactgaaaaa aaatatatcg ttgcgctcga
    14401 ccagggcacc accagctccc gcgcggtcgt aatggatcac gatgccaata tcattagcgt
    14461 gtcgcagcgc gaatttgagc aaatctaccc aaaaccaggc tgggtagaac acgacccaat
    14521 ggaaatctgg gccacccaaa gctccacgct ggtagaagtg ctggcgaaag ccgatatcag
    14581 ttccgatcaa attgcagcta tcggtattac gaaccagcgt gaaaccacta ttgtctggga
    14641 aaaagaaacc ggcaagccta tctataacgc cattgtctgg cagtgccgtc gtaccgcaga
    14701 aatctgcgag catttaaaac gtgacggttt agaagattat atccgcagca ataccggtct
    14761 ggtgattgac ccgtactttt ctggcaccaa agtgaagtgg atcctcgacc atgtggaagg
    14821 ctctcgcgag cgtgcgcgtc gtggtgaatt gctgtttggt acggttgata cgtggcttat
    14881 ctggaaaatg actcagggcc gtgtccatgt gaccgattac accaacgcct ctcgtaccat
    14941 gttgttcaac atccataccc tggactggga cgacaaaatg ctggaagtgc tggatattcc
    15001 gcgcgagatg ctgccagaag tgcgtcgttc ttccgaagtg tacggtcaga ctaacattgg
    15061 cggcaaaggc ggcacgcgta ttccaatctc cgggatcgcc ggtgaccagc aggccgcgct
    15121 gtttggtcag ttgtgcgtga aagaagggat ggcgaagaac acctatggca ctggctgctt
    15181 tatgctgatg aacactggcg agaaagcggt gaaatcagaa aacggcctac tgaccaccat
    15241 cgcctgcggc ccgactggcg aagtgaacta tgcgttggaa ggtgcggtgt ttatggcagg
    15301 cgcatccatt cagtggctgc gcgatgagat gaagttgatt aacgacgcct acgattccga
    15361 atatttcgcc accaaagtgc aaaacaccaa tggtgtgtat gtggttccgg catttaccgg
    15421 gctgggtgcg ccgtactggg acccgtatgc gcgcggggcg attttcggtc tgactcgtgg
    15481 ggtgaacgct aaccacatta tacgcgcgac gctggagtct attgcttatc agacgcgtga
    15541 cgtgctggaa gcgatgcagg ccgactctgg tatccgtctg cacgccctgc gcgtggatgg
    15601 tggcgcagta gcaaacaatt tcctgatgca gttccagtct gatattctcg gcacccgcgt
    15661 tgagcgcccg gaagttcgcg aagtcaccgc attgggtgcg gcctatctcg caggcctggc
    15721 ggttggcttc tggcagaacc tcgacgagct gcaagagaaa gcggtgattg agcgcgagtt
    15781 ccgtccaggc atcgaaacca ctgagcgtaa ttaccgttac gcaggctgga aaaaagcggt
    15841 caaacgcgcg atggcgtggg aagaacacga cgagtaatgt caggcacctg aatcgattgc
    15901 ctgatgctga tcgcctgatg cgacgcttac gcgtcttatc aggcctacga gccaatgcca
    15961 ttttgtgtag gcgggataag gcgttcacgc cgcatccggc atcacgcaga ctctgaaacc
    16021 gtgcgggggc aaccccgcac acatcaataa tcccaccctt cccctgtgct acacttcgcg
    16081 ccattcctta ctgcttagag tttgctatga gacgagaact tgccatcgaa ttttcccgcg
    16141 tcaccgaatc tgcggcgctg gctggctaca aatggttagg acgcggcgat aaaaacaccg
    16201 cggacggcgc ggcggtaaac gccatgcgta ttatgctcaa ccaggtcaac attgacggca
    16261 ccatcgtcat tggtgaaggt gaaatcgacg aagcaccgat gctctacatt ggtgaaaaag
    16321 tcggtactgg tcgcggcgac gcggtagata ttgctgttga tccgattgaa ggcacgcgca
    16381 tgacggcgat gggtcaggct aactcgctgg cggtgctggc agtaggcgat aaaggctgtt
    16441 tcctcaacgc gccagatatg tatatggaga agctgatcgt cggaccggga gccaaaggca
    16501 ccattgatct gaacctgccg ctggcggata acctgcgcaa tgtcgcggcg gcgctcggca
    16561 aaccgttgag cgaactgacg gtaacgattc tggctaaacc acgccacgat gccgttatcg
    16621 ctgaaatgca gcaactcggc gtacgcgtat ttgctattcc ggatggcgac gttgcggcct
    16681 caattctcac ctgtatgcca gacagcgaag ttgacgtgct gtacggaatt ggtggcgcgc
    16741 cggaaggcgt ggtttctgcg gcggtgatcc gcgcattaga tggcgacatg aacggtcgtc
    16801 tgctggcgcg tcatgacgtc aaaggcgaca acgaagagaa tcgtcgcatt ggcgagcagg
    16861 agctggcacg ctgcaaagcg atgggcatcg aatccggtaa agtattgcgt ctgggcgata
    16921 tggcgcgcag cgataacgtc atcttctctg ccaccggtat taccaaaggc gatctgctgg
    16981 aaggcattag ccgcaaaggc aatatcgcga ctaccgaaac gctgctgatc cgcggcaaat
    17041 cacgcaccat tcgccgcatt cagtctatcc actatctgga tcgcaaagac ccggaaatgc
    17101 aggtgcacat cctctgattg atttgatcga ttgagccttc cagtccttcg ggactggaat
    17161 ttttttgttc ggagaacgaa gataaggcaa ttcaatcaaa acaggagaaa aacatggctg
    17221 attgggtaac aggcaaagtc actaaagtgc agaactggac cgacgccctg tttagtctca
    17281 ccgttcacgc ccctgtgcat ccgtttaccg ccgggcaatt taccaagctt ggccttgaaa
    17341 tcgacggcga acgcgtccaa cgcgcctact cctatgttaa ctcgcccgat aatcccgatc
    17401 tggagtttta cctggtcacc gtccccgatg gcaaattaag cccacgattg gcggcactga
    17461 aaccgggcga tgaagtgcag gtggttagcg aagcagctgg cttctttgtt ctggatgaag
    17521 taccagattg cgaaacgcta tggatgctgg caaccggtac agcgattggc ccttatttat
    17581 cgattctgca actaggtaaa gatttagatc gcttcaaaaa tctggtcctg gttcacgccg
    17641 cacgttatgc cgccgactta agctatttgc cactgatgca agaactggaa aaacgctacg
    17701 aaggcaaact gcgcattcag acggtggtca gtcgggaaac ggcagcgggg tcgctcaccg
    17761 gacggatacc ggcattaatt gaaagtggtg aactggaaag cgcgattggc ctgccgatga
    17821 ataaagaaac cagccatgtg atgctgtgcg gcaatccaca gatggtgcgc gatacgcaac
    17881 agttgctgaa agagacccgg cagatgacga aacatttacg tcgccgaccg ggtcacatga
    17941 cagcggagca ttactggtaa gcggttactt atcgataaac ggcacgatga gcaaatccgc
    18001 actcaactta ttgatcatcc cgcgatatgc cggcatcaaa cggttgataa atgagtggtg
    18061 atgaccacag acaaggaggt cgcactgctc tttttgcata atttccagca gtgtttccgg
    18121 catttctccg cgttcaatac gcagttttgt cttcggccat tgaatatttt tcgtcagttt
    18181 atacagcttg ttatccgact tattcttcaa caattgaaga atatcttctg ttgcagggaa
    18241 gtagataccc ggatacaact cgcttaagcc atcatcaata tgaattaacg tcaggtgagc
    18301 gtcattatgt ctggcgagct ccaaggcttt attcaccagt aaggcatctt cttcattccc
    18361 ggaaattgcc acgccaatgt gtttataagc catgtttaac tccttctaaa gcaactccat
    18421 caagctaacg aacgcagtga taactcaaaa ataatcatct cggcctggca tgagaaggtg
    18481 aaggccgcat caagctcaac ttgcccctcc agatccttaa tcttataggc cacgctggcc
    18541 ccttcatctg cagaggatgc agcggcggca agctctttca gtttggtcat aaaggactct
    18601 gcttcttcgc gggtggcaaa aaagcgcgaa aacttactgg tacagttgtc gttatcaatc
    18661 accgtaccga tatcaatcgc acatccttta gtactgcatt tatctacgac atctttcata
    18721 gggcacctct gtatattcgc ctctctgtcg attacgactt aagaataatg cgccgttaag
    18781 ggaagaacaa acaagtaatg atcttatgga ttaccatttt ttttgatatt gcgtaaggtg
    18841 aagagttcag aaggattttc cgggtattgt catttgcatc gtggaaataa caatcccgga
    18901 aaaaaatagc agtaagagat atattaaagg ttaattactt gatttattgt cggctttata
    18961 cttcacatcc tgagtatctt taccatattt attttcgcct tgtgtgccaa caaacgcacc
    19021 aagatcgata agcatcatca ccagaatcaa cgtcgggaca aaacgcccta ccgcccattg
    19081 ccagacaccc ggtaaaatcg cccagttacc cgccagtagc atccacgcca caatcatcag
    19141 aaatgcccat gcgccggaac gcccgcgatc atgcaagcgc ttaacggtta ctgccgctgt
    19201 tggccagagc aagcacacaa ggcaaaacgc cgcggtctga atatcgagta aatttttacc
    19261 cgccagtgag aaaagcacca gcatgcctgc gaaccacagg cctatccaaa tccagaaatc
    19321 acggcgtcca atacgccctt taaatgagaa taaccattgc tgtatggtca tgtaagttcc
    19381 ttgatggttg tcttttccag gattctaccc ttttgacaag ggcgacagat atcgttttaa
    19441 tcggagccag tcataacaaa aggtactgtc aatgaagcca gggtgtacgc tgttttttct
    19501 cttatgttct gcattaaccg ttacaacaac ggcgcatgca caaacaccag atacggcaac
    19561 gaccgcgcct tatctgctgg ctggagcccc tactttcgat ctctccatca gccagtttcg
    19621 agaagacttt aacagccaga atcccagcct gccactgaac gaatttcgtg ccatcgacag
    19681 cagtcccgac aaagccaatc tcactcgtgc tgccagtaaa attaacgaga acttgtatgc
    19741 ttctacagcg ctggagcgcg gtaccttaaa aatcaaaagc attcaaatga catggctacc
    19801 catccagggg ccagagcaaa aagccgcgaa agcgaaagct caggaataca tggcagcggt
    19861 gatccgcaca ctcaccccat taatgaccaa aacacaaagc cagaaaaaac tgcagtcgct
    19921 actaacggcg gggaaaaaca aacgttatta caccgagaca gaaggtgcac tgcgctatgt
    19981 tgtcgcggac aacggcgaaa aggggctgac cttcgctgtt gaaccgatta agctggcgct
    20041 atctgaatcg cttgaaggtt tgaataaatg acaaaaagca aagcctttgt gccgatgaat
    20101 ctctatactg tttcacagac ctgctgccct gcggggcggc catcttcctt tattcgctta
    20161 taagcgtgga gaattaaaat gcgacatcct ttagtgatgg gtaactggaa actgaacggc
    20221 agccgccaca tggttcacga gctggtttct aacctgcgta aagagctggc aggtgttgct
    20281 ggctgtgcgg ttgcaatcgc accaccggaa atgtacatcg atatggcgaa gcgtgaagct
    20341 gaaggcagcc acatcatgct gggtgcgcaa aacgtggacc tgaacctgtc cggcgcattc
    20401 accggtgaaa cctctgctgc tatgctgaaa gacatcggcg cacagtacat catcatcggt
    20461 cactctgaac gtcgtactta ccacaaagaa tctgacgaac tgatcgcgaa aaaattcgcg
    20521 gtgctgaaag agcagggcct gactccggtt ctgtgcatcg gtgaaaccga agctgaaaac
    20581 gaagcgggca aaactgaaga agtttgcgca cgtcagatcg acgcggtact gaaaactcag
    20641 ggtgctgcgg cattcgaagg tgcggttatc gcttacgaac ctgtatgggc aatcggtact
    20701 ggcaaatctg caactccggc tcaggcacag gctgttcaca aattcattcg tgaccacatc
    20761 gctaaagttg acgctaacat cgctgaacaa gtgatcattc agtacggcgg ctctgtaaac
    20821 gcgtctaacg ctgcagaact gtttgctcag ccagacatcg acggcgcgct ggttggcggt
    20881 gcgtctctga aagctgacgc tttcgcagta atcgttaaag ctgcagaagc ggctaaacag
    20941 gcttaagtct gacaggtgcc ggatttcata tccggcactt actttcctta actcttcgcc
    21001 ttaacgcaaa atctcacact gatgatcctg aatttcctcg gctgaagcac ggttaagcgt
    21061 cagtagattt cgttgtgtcg ccagcaatac aaatgagtta tcactctgcc gtaccatcgc
    21121 cagcccgtag cttcccatat gttcccgcgc ctcaggtact tcttctgcca gcatcataaa
    21181 tgggctgcgt tgtaccagtt cgctttccgt tacccgacgc gccaggtatt catgcccgcg
    21241 caaaccacct ggcagtggca accagcggct gctgatgttc gccagattgt tatccagctg
    21301 ttcgcgcaca tcaggacgaa tacaagagat atgaatatga aaatggtttt gcgtacgccc
    21361 ggtgcgggag ttgatcgcca aagaaaccgc gcgatcggga accggctggc cgtatttttt
    21421 gctcataaaa tcacgcgcct gccaggccaa ccaaaagaag ttcggcgttg aaggatcggt
    21481 caacaaagga ctttcagtac cgttaatacg atacgttggc atcaacagat attgcagtgg
    21541 gccattaaga tcttttaaaa ccacgtatcc ggcattgggt ttgacttccg cacatggcga
    21601 aggattttga ttttgctgct gattgggcaa acattcctca aggacaatct tacgtaatgt
    21661 atccgactct tcaccggtta atttccagta accaataccg gcagccacaa cggcgataac
    21721 tatcatcacc aaaaaaagaa gacccgcttt tttcatcttt ttttccctgt acctcaaaga
    21781 gatgcaaggg taacgcaaaa tcgtgacaaa taaaaaaccc ggtcgcgaga aaccaccggg
    21841 tttgataatt atcctgggga aatcagcgtt tgctgatctg atcgaacgta ccgccgttag
    21901 caaaatgctc tttttgcgct ttcgtccagc cgccgaactc ttcatcaatg gtgaataact
    21961 tcagctttgg aaacgcattt tcgtactttt tcgccacctc agcgtcgcgc ggacggtagt
    22021 agtttttcgc ggcaatttcc tgaccttctg gcgagtagag atatttcagg taggcttccg
    22081 ccacctcttt agtacctttt ttctcgacca ctttatcgac caccgacacg gttggctctg
    22141 cgaggataga ctcactcggc gtgacgattt cgaatttatc tttccccagt tcattcgctg
    22201 ccagcagagc ttcgttttcc caggaaatca gtacatcgcc aattccgcgc tcgacaaaag
    22261 tgttagtgga gccgcgcgcg ccagaatcca gaacttcgac gtttttatac agtgcccgaa
    22321 caaaatcctg tgcttttgcc tgatcgttgt tgttgtgatg cagcgcgtag ccccaggctg
    22381 ccaggtagtt ccagcgcgcg ccaccagagc ttttcggatt aggcgtgatc accgaaacac
    22441 ccagtttaat cagatcgttc cagtcatgga tctgcttcgg atttccctta cgtaccagga
    22501 aaacaatggt ggaagtgtac ggtgcggagt tatccggcag acgtttgatc cactctttat
    22561 caatccgccc gcgttccgca atcgcgtcca cgtcataggc cagagccagc gtgacaacat
    22621 cagcttcaat accgttgatt accgacgttg cttgtttgcc tgaaccaccg tgtgactgac
    22681 gaatcaccac gttatcgcca gtttgctgtt tccagtgggc gctgaatgcc ttgttgtact
    22741 gttcgtacaa ttcgcgcgtt ggatcatatg aaacgttaag aagctgaata tcctttgcca
    22801 taacgctggt tgccgccagc aaaaatgtta accctacgcc ccacttgttc atcgcccgac
    22861 tctcttatgt tgtgttgtga tgagcaaagc gtgccagaag gttaaccaaa cattaaagaa
    22921 taaaaaaaga ttggctataa cttgcgggta tatgttgggg gttaaaaagg cggaagaaat
    22981 ccgcctcata ttgctgacaa agtgcgcttt atcgatgccg gatgcggcgc gaacgcctta
    23041 tccggcctac aaaagtttgt aaattcaata aattgtagaa ttcacgtagg cctgataagc
    23101 gaagcgcatc aggcattttt gcttctgtca tcggtttcag gctaaaggaa tctgcctttt
    23161 tccgaaatca ttagtacagt tttttcgcgc agtccagcca gtcgcctttg aacggacgct
    23221 tcatgttttc gatagcgtcg atgatgtcgt ggtgaaccag ctgttcgttc tgtataccta
    23281 cgcaacgacc gccgtaacct gccagcagca gatcgatagc gtaagcgccc atacgggaag
    23341 ccagaatacg gtcgtaaggc accggagaac caccgcgctg gatgtggccc agcacagttg
    23401 cgcgggtttc acgaccggtt tctttctcga tgaaatgcgc cagttcgtca acatcacaca
    23461 tatgttcggt aatcgccacg atcgcgtgtt ttttaccttt cgcgataccc gctttgattt
    23521 cgtttaccag gtcttcacgg ctgaattcaa cttccggaac cacaacgaat tcacagccac
    23581 cggcaatggc cgcagccagc gtcagatcgc cacaataacg gcccatcact tccaccacgg
    23641 aaatacgctg gtgagaagaa gaggtgtcac gcagacggtc gatcgcttct acaacggtgc
    23701 tcagcgcagt gaagaaaccg atagtgtagt cagtgccttt gatgtcgttg tcgatagtgc
    23761 ccggcaggcc gatgcacggg aagcccattt cggtcagacg cattgcaccc atgtaggaac
    23821 cgtcaccgcc gataaccacc agcgcatcga tcccacgttt tttcaggttt tcgatagcca
    23881 cggcgcggat gttctcgtcg cggaattccg ggaaacgcgc agaaccgagg aacgtaccgc
    23941 cacggttgat catgtcagaa acgctgtaac ggtctagctg taccatacgg tcttcataca
    24001 gacccagata gccgtcataa atgcccatta cttccagacc ttctgtcagc gcagaacgaa
    24061 caaccccgcg aattgcggcg ttcatgcctg gcgcatcacc gccgcttgtc aacacaccga
    24121 ttttcttaat catgactacc tctgaacttt ggaatgcaaa ataaaatctg ttgccggaag
    24181 tcttcttgca catcgaagtg atccaacgaa tgtgcaaata gtataacaat cacttcctgc
    24241 tgaattgatt caggtcaggc caaatggcgg tattttatac acaaaatgcg ggtctggctc
    24301 tcttttatac tgattatgaa agcatagacc gtttaccctc cctaggtacg acggaacagg
    24361 ggtcctgatg gataatcaca tccgatcccg gaaaacgccg taaaatagcc tgctctacct
    24421 gatccgccac catatgtgcc tgaaccaaag gcagagagtc ttccatttcc aaatgaatct
    24481 gaataaagcg ggtcggccct gactgccgcg tgcgaagatc gtgagcgccg ctaacacccg
    24541 gccaggaagt cacgatatca ataatttctt gccgttcctc atcaggtaat gcgcgatcca
    24601 gtaatgactg taccgcctca tatcccatgc gtaacgcgct atataaaata tagatgccga
    24661 ttcccaatgc aaacagagca tcggcgcgat gccagccgta ccaggacaac cccagcgcca
    24721 gcagaattgc gccgttcatc ataacatcag actggtaatg tagcatatca gcccgcaccg
    24781 cctggctttg cgtccggcgc accacccaac gctgaaacga gacaaggata atcgtacaaa
    24841 ttagcgccac aattgtcacg ataaccccga cgcctggatc tgtcatcggt gttggagata
    24901 tcagatgttg aatgcctgtc aaaaacagga atagtgccga accggagata aacatacttt
    24961 gcgccagcgc cgcgagggac tcagctttac cgtgaccaaa cgagtgatta tcgtcggcag
    25021 gttgcaggga atatcgcacc accaataaat tcgtcaacga cgcgccgata tccaccagcg
    25081 aatccaccag cgcggcgaga atactcaccg acccggtata ccaccatgca aaaattttaa
    25141 tcagcaatag cagtgaagcc atcgccgtcg cagcaatcgc cgcccgactg accagccgtc
    25201 cataagattg attcataaat actcccgcta tcaactgacg ctagtataac ggaagcaaat
    25261 catctgcaat gcattaagca gcaggcaaat tgaggataaa aaaaaccccc acatcatgtg
    25321 ggggaagaca gggatggtgt ctatggcaag gaaaacaggg tttactactg ggaacgtgag
    25381 ttgctactac tcaatagctt caacgatgaa cttttttgcc attgcgtcac gtcacgcaac
    25441 tgctccattc gttgttgatg tttctcgttt aaaaccgctt gctgctccgg cgttaacagg
    25501 cgatacattt ggttgcggac ttttgccatc tcaacctgac gagcaatttg ctcattcgcc
    25561 attttttctg cctgtgcgcg cacagcgttt tcatcaaaat tttctgcggt gacaagacga
    25621 tgcattgtct ccagttcgct aacattaaca ggaggctgtt cgtgccgggc ctgttgcata
    25681 agatctcgca tctgctgacg ctgatgttcg gttaaactta tgccgtcgaa catatggctc
    25741 tgcgtactgc gctgcgtaag ttcttcaccc ggatgccagt tatcgcctga accgacttca
    25801 gcagcgtggc ttaatgaact gactgccagc gttgaggcca tgacggcagc ggtaactatg
    25861 cgcatcattt gctcccaaaa tctttctgtc gcgattcaac gatagagagt ttacgattca
    25921 ggctgcaaac atgcgtcagg gggtgtaaaa caacgtaaag tcatggatta gcgacgtctg
    25981 atgacgtaat ttctgcctcg gaggtattta aacaatgaat aaaatcctgt tagttgatga
    26041 tgaccgagag ctgacttccc tattaaagga gctgctcgag atggaaggct tcaacgtgat
    26101 tgttgcccac gatggggaac aggcgcttga tcttctggac gacagcattg atttactttt
    26161 gcttgacgta atgatgccga agaaaaatgg tatcgacaca ttaaaagcac ttcgccagac
    26221 acaccagacg cctgtcatta tgttgacggc gcgcggcagt gaacttgatc gcgttctcgg
    26281 ccttgagctg ggcgcagatg actatctccc gaaaccgttt aatgatcgtg agctggtggc
    26341 acgtattcgc gcgatcctgc gccgttcgca ctggagcgag caacagcaaa acaacgacaa
    26401 cggttcaccg acactggaag ttgatgcctt agtactgaat ccaggccgtc aggaagccag
    26461 cttcgacggg caaacgctgg agttaaccgg tactgagttt accctgctct atttgctggc
    26521 acagcatctg ggtcaggtgg tttcccgtga acatttaagc caggaagtgc tgggcaaacg
    26581 cctgacgcct ttcgaccgcg ctatcgatat gcacatttcc aacctgcgtc gtaaactgcc
    26641 ggatcgtaaa gatggtcacc cgtggtttaa aaccttgcgt ggtcgcggct acctgatggt
    26701 ttctgcttca tgataggcag cttaaccgcg cgcatcttcg ccatcttctg gctgacgctg
    26761 gcgctggtgt tgatgttggt tttgatgtta cccaagctcg attcacgcca gatgaccgag
    26821 cttctggata gcgaacagcg tcagggtctg atgattgagc agcatgttga agcggagctg
    26881 gcgaacgatc cgcccaacga tttaatgtgg tggcggcgtc tgttccgggc gattgataag
    26941 tgggcaccgc caggacagcg tttgttattg gtgaccaccg aaggccgcgt gatcggcgct
    27001 gaacgcagcg aaatgcagat cattcgtaac tttattggtc aggccgataa cgccgatcat
    27061 ccgcagaaga aaaagtatgg ccgcgtggaa ctggtcggtc cgttctccgt gcgtgatggc
    27121 gaagataatt accaacttta tctgattcgt ccggccagca gttctcaatc cgatttcatt
    27181 aacttactgt ttgaccgccc gctattactg ctgattgtca ccatgttggt cagtacgccg
    27241 ctgctgttgt ggttggcctg gagtctggca aaaccggcgc gtaagctgaa aaacgctgcc
    27301 gatgaagttg cccagggaaa cttacgccag cacccggaac tggaagcggg gccacaggaa
    27361 ttccttgccg caggtgccag ttttaaccag atggtcaccg cgctggagcg catgatgacc
    27421 tctcagcagc gtctgctttc tgatatctct cacgagctgc gcaccccgct gacgcgtctg
    27481 caactgggta cggcgttact gcgccgtcgt agtggtgaaa gcaaggaact ggagcgtatt
    27541 gaaaccgaag cgcaacgtct ggacagcatg attaacgacc tgttggtgat gtcacgtaat
    27601 cagcaaaaaa acgcgctggt tagcgagacc atcaaagcca atcagttgtg gagtgaagtg
    27661 ctggataacg cggcgttcga agccgagcaa atgggcaagt cgttgacagt taacttcccg
    27721 cctgggccgt ggccgctgta cggcaacccg aacgccctgg agagtgcgct ggaaaacatt
    27781 gttcgtaatg ccctgcgtta ttcccatacg aagattgaag tgggctttgc ggtagataaa
    27841 gacggtatca ccattacggt ggacgacgat ggtcctggcg ttagcccgga agatcgcgaa
    27901 cagattttcc gtccgttcta tcggaccgat gaagcgcgcg atcgtgaatc tggcggtaca
    27961 ggtttgggac tggcgattgt tgaaaccgcc attcagcagc atcgtggctg ggtgaaagca
    28021 gaagacagcc cgctgggcgg tttacggctg gtgatttggt tgccgctgta taagcggagt
    28081 taaactccgc atttgtaggc aggataaggc gtttacgccg catccggcat ttgagcagga
    28141 tgcctgatgc gacgctgata gcgtcttatc aggcctacac tccgggggtt attttcccca
    28201 caaccggcgg gaaaaatctt cgatcttgcc gctcagtcgg cgcttttgca tcgttcgcgt
    28261 ccagcttttc gataacccgg cagcggagag taaacggtga tactgatcgt catcaaacgg
    28321 catatgccat gcgatggcag cagcctcctg cacggtcaca tcgctgacac gggaaaccaa
    28381 ctccagcggc gcgtccgcag acactttccc tggcgctatc acgctataca accatcccac
    28441 tttacccgtg ttttgcatca actgcgctat atcgctgata tcaaaatggt aattgagttt
    28501 atagcaaggc gagcgcggct ggctgacctg aattaatgcc tctccccagc ggaaaatatc
    28561 gccaatataa acattacttt ccgtgagacc gtcggttgag agattttcgc caaacgcagg
    28621 cgcaacaaac aactccgcct gttccggaaa ttcccgcgcc cagtagagat aatgctcacg
    28681 aggataatga cacagcgctc tgtctggtcc gccgtgaacc tttttctccg cctgctcgtc
    28741 accttccagc cccagctccg ttagcatcaa ctcaccatca acctggattt tggcaattgc
    28801 gctgggtttg ccttcgggat aagcctgaat cttgcctgtg tatacatcaa ccggatatcg
    28861 catctgttcc ctctccacga gaaatttccc cattagacca tatttatttt atgtcaggcg
    28921 tgctgttttc gcggtaagta tccaggcagg aactcaatag caaaccggac gaacgttagt
    28981 tcatccggct gtggggatta aggaagcagg tttatttcac cgtacctaaa atttcgattt
    29041 tcgccgctct ggctttgact ttacgtgacc agatagaggt aagaattggc accagaatcg
    29101 aagtcacaat gactgccgtc gctaccagcg aagttgctgc cggagccatc ggtttaaacg
    29161 caggcaccat ttctgcaatc agcacaggtg tcgctaccgc ggcccctgcg gaactggaag
    29221 cggcaattcc ggcagtgcca tcgccaccgc caatcaattt atcggcgata atcaacggaa
    29281 taccggtcac gataattact gccacaccca acaggatccc cagcaaacct gtctgggcaa
    29341 ttacagtcaa atcaatggta ttgcctagtg cgaaggcaaa gaatggaatc agcgtctgca
    29401 ccgctttgct gaaaaattct cgtaattcag ggtcaaggtt cccaagggca aagcccacca
    29461 ggaacggtaa tactgcgccg acgaaaacat gcggttcaaa cgaggcaatc ccggcagtgc
    29521 ccagaataat catcgtcatg agcggcccgg actccaacga catcaacaca aatgccccag
    29581 cttcttcttt tgtgccgtac tgctgcatga tggaagcgta aagtccgccg ttggtcatat
    29641 ccatcgccgc caccagcgcc agcgttgaaa gtccggcaaa gaatccaact tcaacaccat
    29701 gttccggaat aatgcgcgag gcaattgccg caaccaccca cgcgacggca attttagtta
    29761 ccaccagcgt accggattta cgcagtaccg ttcccgtcgc gcttaatttt attgacgccc
    29821 ccatgcaaaa aaaccacacc gccagaatgg gcaccgtacc ggtaatcatc ccgttggtga
    29881 atgatccaaa atatttcccc gcccccggcg agaaggtgtg gcacagtgcg ccaaggaata
    29941 gcgggacgag catcatcccc cccgggattt tctcaatcaa gcgttttatc tgcatttcca
    30001 tcacctgtcg gcatattttt aagggctgat gcgtaattcc tcaatggagc atcagtgttg
    30061 atggaaacga agatagacat tcatttaatc aataaaagtg atcaaaacca catcaactga
    30121 aacgctattt tatttttatc ggatcattgt ttttattttg aaattgatca caaaaaaacc
    30181 accgccattg gcgatggttc attttttaag ctgatatgcg gcgtaaacgc ctcactgcag
    30241 cagacggcaa atgattattt tttcgccgcg aaacgtgccg ctgcttcgtc ccagttcacc
    30301 acgttccaga actctttgat gtagtccggg cggcggttct ggaatttcag gtagtaagca
    30361 tgctcccaca catccaggcc cagaatcggg aagccggatg cgccagaaat agcttcaccc
    30421 atcagcgggg agtcctggtt agcagtagaa accaccgcca gtttatcgcc tttcagcacc
    30481 agccatgccc agccggaacc aaagcgggaa gctgccgctt tctcaaattc tgctttgaag
    30541 ttatctacgg aaccgaaatc acgttcgata gccgctttca ggtcaccctg cagggtggtg
    30601 ccttttttca gacctttcca gaacaggctg tggttagcgt gaccgccagc gttgttgcgc
    30661 agtacggttt tcttgtctgc tggcagctgg tccagtttgg tgatcaactc ttcaaccggc
    30721 aggttggcaa attctggcag gctttccaac gccgcgttgg cgttgttgac gtaggtctga
    30781 tggtgtttgg tgtggtggat ttccatggtc tgcttatcga agtgcggttc cagggcatcg
    30841 taagcatacg gcagggatgg cagggtatag ctcatattca tctccagtat tgtcgggcgg
    30901 ccgattgtta atgccgcgta agcagttggt tcattatagt taattaaatg atattgaaaa
    30961 tgattatcaa tgccgtactt ttcgtaaggg tatggttttg caggaaaatg cccgagatgt
    31021 gaagcaaatc acccacttaa tgccgtgatt gccagtaaat cgacaacggc ggcaacaggc
    31081 gaaaggttaa tcgacagcac gatttttaca ctcatctcgt cggagatgtg acgcgacgaa
    31141 aaatgatgag gataagaaga tgagtaacgc gattacgatg gggatatttt ggcatttgat
    31201 cggcgcggcc agtgcagcct gtttttacgc tccgttcaaa aaagtaaaaa aatggtcatg
    31261 ggaaaccatg tggtcagtcg gtgggattgt ttcgtggatt attctgccgt aggccatcag
    31321 cgccctgtta ctaccgaatt tctgggcgta ttacagctcg tttagtctct ctacgctgct
    31381 gcctgttttt ctgttcggcg ctatgtgggg gatcggtaat atcaactacg gcctgaccat
    31441 gcgttatctc ggcatgtcga tgggaattgg catcgccatt ggcattacgt tgattgtcgg
    31501 tacgctgatg acgccaatta tcaacggcaa tttcgatgtg ttgattaaca ccgaaggcgg
    31561 acgcatgacg ttgctcggcg ttctggtggc gctgattggc gtagggattg taactcgagc
    31621 cgggcagttg aaagagcgca agatgggcat taaagccgaa gagttcaatc tgaaaaaagg
    31681 actggtgctg gcggtgatgt gcggcatttt ctctgccggg atgtcctttg cgatgaacgc
    31741 cgcaaaaccg atgcatgaag ccgctgccgc actaggcgtc gatccactgt atgtcgctct
    31801 gccaagctat gttgtcatca tgggcggcgg cgcgatcatc aacctcggtt tctgttttat
    31861 tcgtctggca aaagtgaagg atttatcgct aaaagccgac ttctcgctgg caaaaccgct
    31921 gatcattcac aatgtgttac tctcagcact gggcgggttg atgtggtatc tgcaattctt
    31981 tttctatgcc tggggccacg cccgcattcc ggcgcagtat gactacatca gttggatgct
    32041 gcatatgagt ttctatgtat tgtgcggcgg tatcgtcggg ctggtgctga aagagtggaa
    32101 caatgcagga cgccgtccgg taacggtgtt gagcctcggt tgtgtggtga ttattgttgc
    32161 cgccaacatc gttggcatgg gcatggcgaa ttaatctttc tgcgaattga gatgacgcca
    32221 ctggctgggc gtcatcccgg tttcccgggt aaacaccacc gaaaaatagt tactatcttc
    32281 aaagccacat tcggtcgaaa tatcactgat taacaggcgg ctatgctgga gaagatattg
    32341 cgcatgacac actctgacct gccgcagata ttgattgatg gtcattccag tctgctggcg
    32401 aaattgctga cgcaaaacgc gctcactgca cgatgcctca tcacaaaatt tatccagcgc
    32461 aaagggactt ttcaggctag ccgccaaccg ggtaatcagc ttatccagca acgtttcgct
    32521 ggatgttggc ggcaacgaat cactggtgta acgatggcga ttcagcaaca tcaccaactg
    32581 cccgaacagc aactctgcca tttcgttagc aaacggcaca tgctgactac tttcatgctc
    32641 aagctgaccg ataacctgcc gcgcctgtgc catccccatg ctacctaagc gccagtgtgg
    32701 ttgccctgcg ctggcgctaa atcccggaat cgccccctgc cagtcaagat tcagcttcag
    32761 ccgctccggg caataaataa tattctgcaa aaccagatcg ttaacggaag cgtaggagtg
    32821 tttatcgtca gcatgaatgt aaaagagatc gccacgggta atgcgataag ggcgatcgtt
    32881 gagtacatgc agaccattac cgcgccagac aatcaccagc tcacaaaaat catgtgtatg
    32941 ttcagcaaag acatcttgcg gataacggtc agccacagcg actgcctgct ggtcgctggc
    33001 aaaaaaatca tctttgagaa gttttaactg atgcgccacc gtggctacct cggccagaga
    33061 acgaaattga ttattcgcaa tatggcgtac aaatacgttg agaagattcg cgttattgca
    33121 gaaagccatc ccgtccctga cgaatatcac gcggtgacca gttaaactct cggcgaaaaa
    33181 gcgtcgaaaa gtggttactg tcgctgaatc cgcaatgata ggcgatgtca gtaacgctgg
    33241 cctcgctgtg acgtagcaga tgtcgggctt tcatcagtcg caggcgattc aggtatcgct
    33301 gaggcgtcag tcccgtttgc tgcttaagct gccgatgtag cgtacgcagt gaaagagaaa
    33361 attgatccgc cacggcatcc caattcacct catcggcaaa atggtcctcc agccaggcca
    33421 gaagcaagtt gagacgtgat gcgctgtttt ccaggttctc ctgcaaactg cttttacgca
    33481 gcaagagcag taattgcata aacaagatct cgcgactggc ggtcgagggt aaatcatttt
    33541 ccccttcctg ctgttccatc tgtgcaacca gctgtcgcac ctgctgcaat acgctgtggt
    33601 taacgcgcca gtgagacgga tactgcccat ccagctcttg tggcagcaac tgattcagtc
    33661 cggcgagaaa ctgaaatcga tccggcgagc gatacagcac attagtcaga cacagattat
    33721 cggtatgttc atacagatgc cgatcatggt cgcgcacaaa acagaccgtg ccaccggtga
    33781 tggtataggg ctgcccatta aacacatgaa tacccgtgcc atgttcgaca atcacaattt
    33841 catgaaaatc atgatgatgt tcaggaaaat ccgcctgcgg gagccggggt tctatcgcca
    33901 cggacgcgtt accagacgga aaaaaatcca cactatgtaa tacggtcata ctggcctcct
    33961 gatgtcgtca acacggcgaa atagtaatca cgaggtcagg ttcttacctt aaattttcga
    34021 cggaaaacca cgtaaaaaac gtcgattttt caagatacag cgtgaatttt caggaaatgc
    34081 ggtgagcatc acatcaccac aattcagcaa attgtgaaca tcatcacgtt catctttccc
    34141 tggttgccaa tggcccattt tcctgtcagt aacgagaagg tcgcgaattc aggcgctttt
    34201 tagactggtc gtaatgaaat tcagcaggat cacattatga cctttcgcaa ttgtgtcgcc
    34261 gtcgatctcg gcgcatccag tgggcgcgtg atgctggcgc gttacgagcg tgaatgccgc
    34321 agcctgacgc tgcgcgaaat ccatcgtttt aacaatgggc tgcatagtca gaacggctat
    34381 gtcacctggg atgtggatag ccttgaaagt gccattcgcc ttggattaaa caaggtgtgc
    34441 gaggaaggga ttcgtatcga tagcattggg attgatacct ggggcgtgga ctttgtgctg
    34501 ctcgaccaac agggtcagcg tgtgggcctg cccgttgctt atcgcgatag ccgcaccaat
    34561 ggcctaatgg cgcaggcaca acaacaactc ggcaaacgcg atatttatca acgtagcggc
    34621 atccagtttc tgcccttcaa tacgctttat cagttgcgtg cgctgacgga gcaacaacct
    34681 gaacttattc cacacattgc tcacgctctg ctgatgccgg attacttcag ttatcgcctg
    34741 accggcaaga tgaactggga atataccaac gccacgacca cgcaactggt caatatcaat
    34801 agcgacgact gggacgagtc gctactggcg tggagcgggg ccaacaaagc ctggtttggt
    34861 cgcccgacgc atccgggtaa tgtcataggt cactggattt gcccgcaggg taatgagatt
    34921 ccagtggtcg ccgttgccag ccatgatacc gccagcgcgg ttatcgcctc gccgttaaac
    34981 ggttcacgtg ctgcttatct ctcttctggc acctggtcat tgatgggctt cgaaagccag
    35041 acgccattta ccaatgacac ggcactggca gccaacatca ccaatgaagg cggggcggaa
    35101 ggtcgctatc gggtgctgaa aaatattatg ggcttatggc tgcttcagcg agtgcttcag
    35161 gagcggcaaa tcaacgatct tccggcgctt atctccgcga cacaggcact tccggcttgc
    35221 cgcttcatta tcaatcccaa tgacgatcgc tttattaatc ctgagacgat gtgcagcgaa
    35281 attcaggctg cgtgtcggga aacggcgcaa ccgatcccgg aaagtgatgc tgaactggcg
    35341 cgctgcattt tcgacagtct ggcgctgctg tatgccgatg tgttgcatga gctggcgcag
    35401 ctgcgcggtg aagatttctc gcaactgcat attgtcggcg gaggctgcca gaacacgctg
    35461 ctcaaccagc tatgcgccga tgcctgcggt attcgggtga tcgccgggcc tgttgaagcc
    35521 tcgacgctcg gcaatatcgg catccagtta atgacgctgg atgaactcaa caatgtggat
    35581 gatttccgtc aggtcgtcag caccaccgcg aatctgacca cctttacccc taatcctgac
    35641 agtgaaattg cccactatgt ggcgcggatt cactctacac gacagacaaa ggagctttgc
    35701 gcatgaccac tcaactggaa caggcctggg agctggcgaa acagcgtttc gcggcagtag
    35761 ggattgatgt cgaggaggcg ctgcgccaac ttgatcgttt acccgtttca atgcactgct
    35821 ggcagggtga tgatgtttcc ggttttgaaa acccggaagg ttcgctgacc ggagggattc
    35881 aggctactgg caattatccg ggcaaagcgc gtaatgccag tgagctacgt gccgatctgg
    35941 aacaggctat gcggctgatt ccggggccaa aacggcttaa tttacatgcc atctatctgg
    36001 aatcagacac gccagtctcg cgcgaccaga tcaaacctga gcacttcaaa aactgggttg
    36061 aatgggcgaa agccaatcag ctcggtctgg attttaaccc ctcctgtttc tcgcatccgc
    36121 taagcgccga tggctttacg ctttcccatg ccgacgacag cattcgccag ttctggattg
    36181 atcactgcaa ggccagccgc cgcgtttcgg cctattttgg tgagcaactc ggcacaccgt
    36241 cggtgatgaa catctggatc ccggatggca tgaaagatat caccgttgac cgtctcgctc
    36301 cgcgccagcg tctgctggca gctctggatg aggtgatcag cgagaagctg gatccggcgc
    36361 accatatcga cgccgttgag agcaaattgt ttggcattgg cgcagagagc tacacggttg
    36421 gctccaatga gttttacatg gggtatgcca ccagccgcca gactgcgctg tgcctggacg
    36481 ccgggcattt ccacccgact gaagtgattt ccgacaagat ttccgccgcc atgctgtatg
    36541 tgccgcagtt gctgctgcac gtcagccgtc cggttcgctg ggacagcgat cacgtagtgc
    36601 tgctggatga tgaaacccag gcgattgcca gtgagattgt tcgtcacgat ctgtttgacc
    36661 gggtgcatat cggcctcgac ttctttgatg cctctatcaa ccgtattgct gcgtggatca
    36721 ttggtacacg caatatgaaa aaagccctgc tgcgtgcgtt gctggaacct accgctgagc
    36781 tgcgcaagct ggaagcggcg ggcgattaca ctgcgcgtct ggcactgctg gaagagcaga
    36841 aatcgttgcc gtggcaggcg gtctgggaaa tgtattgcca acgtcacgat acgccagcag
    36901 gtagcgaatg gctggagagc gtgcgggcat atgagaaaga aactttgagt cgccgcgggt
    36961 aaacactgcc ggatgcggca caagtgcctt atccggccta caggccggca atagttgtag
    37021 gcctgataag acgcgacagc gtcgcatcag gcattgattg ccggatgcgg cacgagcgcc
    37081 ttatccggcc tacgggtcgt gcatctgaca acaccgaatt tacaggaaca cagaacatgc
    37141 aaaacattac ccagtcctgg tttgtccagg gaatgatcaa agccaccacc gatgcctggc
    37201 tgaaaggctg ggacgagcgc aacggcggca acctgacgct gcgcctggat gacgccgata
    37261 tcgcgcttta tcacgacaac ttccacccac aaccgcgcta tattccgctc agccaaccca
    37321 tgcctttact ggcaaatacg ccgtttattg tcaccggctc cggcaaattc ttccgtaacg
    37381 tccagctcga tcctgcagct aacttaggcg tcgtaaaagt cgacagcgat ggcgcgggct
    37441 accatattct ctgggggtta accaacgaag ccgttccaac ttctgaactt ccggctcact
    37501 ttctctccca ctgcgagcgc attaaagcca ccaacggtaa agatcgggtg atcatgcact
    37561 gccacgccac caacctgatc gccctcacct atgtacttga aaacgacacc gcggtcttca
    37621 ctcgccaact gtgggaaggc agcaccgagt gtctggtggt attcccggat ggcgttggca
    37681 ttctgccgtg gatggtaccc ggcacggacg aaatcggcca ggcaactgcc caagagatgc
    37741 aaaaacattc gctggtgttg tggcccttcc acggtgtgtt cggttccgga ccgacgctgg
    37801 atgaaacctt cggtttaatc gacaccgcag aaaaatcagc ggaagtgtta gtgaaggtgt
    37861 attcgatggg cggcatgaaa cagactatca gccgtgaaga gttgatagcg ctcggccagc
    37921 gtttcggcgt tacaccactc gccagtgcgc tggcgctgta aggagcaaac atgatccgca
    37981 aagcctttgt catgcaggta aaccccgacg cccacgaaga gtatcagcgt cggcataatc
    38041 ccatctggcc ggaactggaa gcagtgttga aatctcacgg tgcgcataac tacgccattt
    38101 acctcgacaa agcgcgtaat ctgctgtttg ccacggtaga gattgaatct gaagaacggt
    38161 ggaatgcggt agccagcact gaaatctgcc aacgttggtg gaaatatatg accgatgtta
    38221 tgcccgctaa cccggataac agcccggtga gtagcgagct gcaagaagtg ttttacctgc
    38281 cttaatgagt tgcggtgccg gaaaatatcc agcaccgcga cttcatcaga agaagtattt
    38341 aaagcgaacg cgtgcgccgt aacggtcatc agtatcatca ccgtgttttt tatcgccgtc
    38401 gctatccaga atggagtaat acgtcccgag gtaaatatta aagttctcca tatccatcac
    38461 gttagcgaac tgataagacg catgaatggt gtgaatggtg tacgtccctt catcatcaat
    38521 ccagcagtcg ttatcacaaa ctacaccgct gaattcatca atcttattgt gcgcgtagat
    38581 ataaccgagc tcgaaacgtt tccacagcgc gttaatcccg gcagtgaaat ctttctcatt
    38641 attagcatct aaataggcgg tatttagatt aaccacaatg ccattgtccg gatccgtttt
    38701 caggccgttc caggtcatgc tcataccata gccggtacga tcggactgat cgacaaaatt
    38761 acccttgcta tcggtataac cataagcatt atttaccacg ttcgcttcca tcgctgcgga
    38821 aacggtgaat tcttccgtcg gcgaccaggc aattactggg cgcagataag caacattttt
    38881 ctgctgctcc atatcgcgtc catgataatt accgtcatta tataaagatg ttccgtcttc
    38941 aagtaacgtg tttaattcaa aataccagtt atcgagttgt ttgctgacga ggaaattacc
    39001 gccagcgtta gagcgcccgc ggccctcttt catcatatag atataaccgc tgccatcgtc
    39061 ataaagatcg ttcgcagtat taccggaatg ttcaacaaag gtatcctgat tcaatgggaa
    39121 catatcgtag gcttcaaaac ggcctacttt gaccttccag tcgttttctt tgccaaagaa
    39181 gaataccgca tcatcgatat ttactgaacc gtgcatatca cccagcggtt gtgcggagaa
    39241 cccggcgaaa taaccgttgt ccatctttcg cataccgtca aaacctaaca gaatacgacc
    39301 attgagattc cactgttcat tagtgggatc gctattcaca tcagcgttgg tcattgccag
    39361 caggccatga ttactttccg catccatatt aaattctacg tcgccgtaga tttttaaatc
    39421 gccataacct gaaagcaaaa gattaggtga ggtggcattt ttctttgcag attccgccgt
    39481 tgcctgtcca tttaccggcg tattgccctg agcggcctta atttcgcgga tctcagcggc
    39541 ttgttgtgtc tgcagctgtt taacctggct ctctgctttt gccgcccgca tttctgccgc
    39601 ttccaggcgt tcttccagca atgccatgcg ttgttccagc attaaatgct gtgtttttgc
    39661 cataccggta ctacatgcca ataataaacc gatggctaaa gataatgtcg attttttcat
    39721 tatttacgtt ccctgaaata aaggagaccc tacgctttcc aaaaggaata cgtatataga
    39781 gaaatataag gtgtgcactt tcgaattttt aatccaactt cagcaggccg atggatagta
    39841 tcccgccgca taatatgagt aaatccgctg atttcctgga acgtgaggaa tataacaata
    39901 gtgccgtttt taactaaata tcgtaaaaaa tattagatat gattcaagct ggctcataag
    39961 ccacaaatat aaataacgtt tagacaaatt atctgagaca tttaaacatt cacaaaatca
    40021 ttaaataata actctttatt cacaagattt attttaccta ctaatatgtg ccttacgccg
    40081 caaaatttat ccttcactct gctttatatt gtcacgactt cacgacatat ccgcgctcat
    40141 cacaaacccg ccccgctatt acaattaact gaaatatttt ttcatggagt ccgattaatg
    40201 gcagctctta ctgcaagctg tattgacctg aatattcagg gcaatggcgc ttattccgtt
    40261 ctgaagcagt tggcgacaat ggcgttacaa aacggtttta tcaccgactc acaccagttc
    40321 ctgcaaaccc tgctgctgcg cgaaaaaatg cactccaccg gttttggttc tggtgtcgcc
    40381 gtgccgcacg gtaaaagcac ctgcgttaaa caaccgttcg tgttattcgc ccgcaaagcg
    40441 caggctattg actggaaagc cagcgacggc gaagacgtca attgctggat ctgcctcggc
    40501 gtaccgcaaa gtggcgaaga ggatcaggtc aaaatcatcg gcacactgtg tcgcaaaatt
    40561 attcaccagg aatttattca tcaactgcaa cagggcgata ccgaccaggt gcttgccttg
    40621 ttaaatcaaa ccctcagctc ataaggaagt ggcgatggag tcatccttac gtattgtcgc
    40681 gatcaccaac tgccccgccg ggatcgctca cacctacatg gtggcggaag ccctggaaca
    40741 gaaagcccgt tctctcggcc ataccattaa agtggaaact caagggtcca gcggcgttga
    40801 aaaccgctta tccagcgaag agattgccgc tgccgattac gtgattctcg ccaccgggcg
    40861 tggcctgagc ggtgacgatc gcgcacggtt tgccgggaaa aaagtttatg agattgccat
    40921 ctcccaggcg ctgaaaaata tcgatcagat tttcagtgaa ttaccgacaa attcgcagct
    40981 ttttgccgca gatagcggcg tgaagctagg caaacaggaa gtacaaagcg gcagcgtgat
    41041 gagccacctg atggcgggcg tctctgcggc gctgccgttt gtcatcggcg gtggtatcct
    41101 ggtggcgctt gccaacatgc tggtgcagtt cggtttgccg tataccgata tgtcgaaagg
    41161 cgcgccgtcg tttacctggg tagttgaatc catcggttac cttggcttca cctttatgat
    41221 ccccatcatg ggcgcttata ttgcctcgtc gattgccgat aaaccggctt tcgctccggc
    41281 gtttctggtt tgctatctgg cgaacgacaa agcgctgctc ggcacccagt cgggtgcggg
    41341 attcctcggc gcggtggtgc tggggctggc gattggctat ttcgtcttct ggttccgtaa
    41401 agtccgtctt ggcaaagcat tacaaccgct gctcggctcc atgcttatcc cattcgttac
    41461 tctgctggtt tttggcgtgc tcacttacta cgttatcggg ccggtgatgt ccgacctcat
    41521 gggcgggcta ctgcacttcc tgaataccat tcctccatca atgaagtttg cggcagcgtt
    41581 tctggtcggt gcgatgctgg cgttcgatat gggcggcccc atcaataaaa ccgcctggtt
    41641 cttctgcttc tcactgctgg aaaaacacat ttatgactgg tacgccatcg tcggcgttgt
    41701 ccccgtcgcg gcgggtcttg cgacgttcat tgcgcccaaa ctgtttaccc gacaggaaaa
    41761 agaggctgcc agtagtgcca ttgtggtcgg tgccacagtc gcaaccgaac cggctattcc
    41821 ttacgctctg gcagctccgt tgccgatgat taccgccaat acgctggcgg gcggcattac
    41881 cggcgtgctg gtgatcgctt tcggaatcaa acgtctggca cctggtttgg gtatctttga
    41941 cccgctgatc ggcctgatgt cgccggtagg ttcgttttat ctggtgctgg cgattggcct
    42001 cgcgctgaac atttcattca ttatcgtcct gaaaggattg tggttacgcc gcaaagcgaa
    42061 agccgcgcaa caggagcttg tccatgaaca ttgagttact gcaacagttg tgcgaagcca
    42121 gcgccgtcag cggcgatgaa caggaagttc gcgacattct gataaacacg ctggaacctt
    42181 gcgttaatga gatcaccttt gatggtctgg gcagctttgt tgcccgtaag ggaaataaag
    42241 gtccaaaagt tgccgttgtc gggcatatgg atgaagtcgg ctttatggtc acccacatcg
    42301 acgagagcgg ttttctgcgc tttaccacca ttggcggctg gtggaatcag tcgatgctca
    42361 accaccgggt aacaatacgc acacacaagg gagtgaaaat ccctggtgtg attggttccg
    42421 tagcgcctca tgcgttaacg gaaaagcaaa agcaacaacc gctgtcattt gatgagatgt
    42481 tcattgatat tggcgcgaac agtcgcgaag aagcgaaaaa acgcggtgcg gaaattggcg
    42541 attttattag cccggaagcc aattttgcct gctggggcga agataaaata gtcggcaagg
    42601 cgctggataa tcgcatcggc tgcgcgatga tggctgagct actacagaca gtaaataacc
    42661 caggaattac gctgtacggc gtcggcagcg tggaagaaga agttgggcta cgcggggcac
    42721 aaacctcggc tgaacacact aaaccggatg tggtgatcgt gctggatacc gccgtcgcgg
    42781 gcgatgttcc gggcattgat aacattaaat acccgctgaa actgggcaac gggccggggc
    42841 tgatgctgtt tgacaagcgc tacttaccca accagaaact ggtggcggcg ttaaaaaact
    42901 gtgccgcaca taacggttta ccgctgcaat tttccaccat gaaaaccgga gcgacggatg
    42961 gcgggcgcta caacgtaatg ggcggagggc gtccggttgt cgcgctgtgt ctgccaactc
    43021 gttatctgca cgctaacagc ggtatgattt caaaagccga ttatgatgct ctgctcacgc
    43081 tgatacggga ttttctgacg accttaactg cggagaaagt caacgcgttt agccagttcc
    43141 gtcaggtgga ttaatgctaa acgaacgcca gttaaagatt gtcgatctgc tggagcaaca
    43201 gccgcgcacg cctggcgagc tggcgcaaca gactggcgtt tcaggcagga ccatcctgcg
    43261 tgatattgac tatctcaact ttacccttaa cggcaaagct cgtatctctg ccagtggcag
    43321 tgcgggctat cagttggaaa tcttcgagcg ccgcagcttt tttcagttgc tgcaaaagca
    43381 cgataacgac gatcggctgc tggcgctgtt attactgaat acctttactc cccgtgcgca
    43441 actcgcctcg gcgcttaatt tgccagaaac ttgggtcgca gagcgtctgc cccggttaaa
    43501 acagcgttat gaacgcactt gttgcctggc cagtcgtccc ggtttgggcc atttcattga
    43561 tgaaacagaa gagaaacgcg ttatcttgct ggcgaacttg ctacgcaaag atccgttttt
    43621 aatacctttg gctggcgtaa cacgagacaa ccttcagcat ttatccacgg cctgcgacaa
    43681 ccaacaccgc tggccgctca tgcaggggga ttatctctcc agcctgattc tggcgattta
    43741 cgccctgcgt aatcaactga ccgatgagtg gccgcaatat cccggtgacg agataaaaca
    43801 aatcgttgaa cataacggtc tgtttcttgg tgataacgct gtaagaaccc tgacaggttt
    43861 gatagagaaa cagcatcagc aagcgcaggt aatttctgcc gatcatgtgc tggggttgct
    43921 gcaaagggtg ccaggtctcg cgtcattgaa tattattgat acgcagctgg ttgagaacat
    43981 taccggtcat ttattacgtt gccttgccgc accagtgtgg attgctgagc atcgccaaag
    44041 cagcatgaat aacctgaaag ccgcctggcc tgcggccttt gatatgagtc tgcactttat
    44101 tacgctactg cgtgaacagc tcgatattcc ccttttcgac agcgatctga tcggtttgta
    44161 ttttgcctgt gcgctggaac gacatcaaaa cgaacgccag ccgatcattt tgctctcgga
    44221 ccagaacgcg attgccacta ttaatcagct cgccattgag cgcgatgttt tacattgtcg
    44281 ggtgattatt gcccgtagct taagcgaact tgttgccatt cgcgaagaga ttgagccgtt
    44341 attgatcatt aacaacagcc attatttact ggatgacgcg gtaaataatt acatcaccgt
    44401 aaaaaatatc attacggctg ccggtatcga acaaataaaa cattttctgg cgacggcatt
    44461 tattcgccag caaccggagc gttttttctc tgcccccgga agttttcatt attcgaatgt
    44521 acgcggtgaa agctggcaac atattacccg gcaaatttgt gcgcaattag tcgcacaaca
    44581 ccatattacc gccgatgaag cgcaacggat catcgcccgc gaaggcgaag gtgaaaacct
    44641 gattgttaat cgcctcgcca tcccacattg ctggagcgaa caggagcgac gttttcgtgg
    44701 attttttatt accctcgccc aaccagttga ggtgaataac gaagtcatta accatgtctt
    44761 gatcgcctgc gccgccgccg atgcgcgtca tgagctaaaa atatttagct atctggcaag
    44821 cgtattgtgt cagcatccgg cagaagttat tgccgggcta acaggatatg aggcatttat
    44881 ggagttactt cacaaggggt gaactgtgtc ggatgcgccg catccgacac tgcatttatc
    44941 atcaaaaacg tttaacgcag gctattatag tcatcaacca tttcgttgta ctcgcgtaac
    45001 gctcgtggaa atgagtcttc gaccatccag cttgtgtttg catcctgaag atgctcttta
    45061 tcccagtcag agtacgggac tttatctctg acgcggcgaa catatttttt agtctcgagt
    45121 tgatactggt ctgccgaatt aatgaacgag aaattcatgc cgcctttatc cgcttctttg
    45181 gcctgcgcca ccagcgtttc cagttcagac actttcttca ttgccgcatc aacatcaaac
    45241 ttctcttgcg atatcaggtt attaatttgt ttcgctgaaa tcatgactgc cagagaatag
    45301 tagtggaatg ttttcccttc tctttcttca atatttttca gttccgcaag ctggcgctta
    45361 tcattaattt cctgaatcgc cgcatgataa gattctgcta caggttcaaa ggcttccaga
    45421 ttttttaaga atgtctggtg cagcgttttc cctttcgcaa acgcatcgtc tttataattt
    45481 tcctgggtgt aatatttatc catttcgtta atggtattac ccaacgccac ggcagcatcg
    45541 atataactca ccgccacgcc atcaattggt tgcagcgcag gcgttaacgc cacagcactt
    45601 tttacgcctt tttcacactc agcgaggttg gattcactaa tgccgtagat gccataaaca
    45661 gtactctctt taccggtagg cccctgttta aaatctttca gccagtcagc ataacgcgcc
    45721 agactgcgct gtaccgggat ctgtaacttg ttgtagcact tgatatagac atccattttt
    45781 tcgtcgaaaa ccgcttgttc atcgaccagc ggttgcgcag gtgtttcagg tttagcatct
    45841 gcttttgcaa cgggagcttc agtaggtttc gcttcaggag ctggtgcggc aggttgacta
    45901 ttggcaggca ggggcgtttc tgtttcggct tttttatcat cacaacccgt cagaccgagg
    45961 gccatgatgg cgactataat agcggaagat aataaattcc tt
//
LOCUS       DAZKIP010000022        45078 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZKIP010000022 DAZKIP010000000
VERSION     DAZKIP010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45078
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.022"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(88..1737)
                     /gene="actP"
                     /locus_tag="ACHOUK_001505"
     CDS             complement(88..1737)
                     /gene="actP"
                     /locus_tag="ACHOUK_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFP9896979.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRNDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1734..2048)
                     /gene="yjcH"
                     /locus_tag="ACHOUK_001506"
     CDS             complement(1734..2048)
                     /gene="yjcH"
                     /locus_tag="ACHOUK_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFP9896980.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPVGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2249..4207)
                     /gene="acs"
                     /locus_tag="ACHOUK_001507"
     CDS             complement(2249..4207)
                     /gene="acs"
                     /locus_tag="ACHOUK_001507"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFP9896981.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINAPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLKLGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDQVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDS
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4600..6036
                     /gene="nrfA"
                     /locus_tag="ACHOUK_001508"
     CDS             4600..6036
                     /gene="nrfA"
                     /locus_tag="ACHOUK_001508"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFP9896982.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6081..6647
                     /gene="nrfB"
                     /locus_tag="ACHOUK_001509"
     CDS             6081..6647
                     /gene="nrfB"
                     /locus_tag="ACHOUK_001509"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFP9896983.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6644..7315
                     /gene="nrfC"
                     /locus_tag="ACHOUK_001510"
     CDS             6644..7315
                     /gene="nrfC"
                     /locus_tag="ACHOUK_001510"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFP9896984.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7312..8268
                     /gene="nrfD"
                     /locus_tag="ACHOUK_001511"
     CDS             7312..8268
                     /gene="nrfD"
                     /locus_tag="ACHOUK_001511"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFP9896985.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLSLVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLAAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LFKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8288..10006
                     /gene="nrfE"
                     /locus_tag="ACHOUK_001512"
     CDS             8288..10006
                     /gene="nrfE"
                     /locus_tag="ACHOUK_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFP9896986.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWKLKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWQYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALLAHAGILLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDV"
     gene            9999..10382
                     /gene="nrfF"
                     /locus_tag="ACHOUK_001513"
     CDS             9999..10382
                     /gene="nrfF"
                     /locus_tag="ACHOUK_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFP9896987.1"
                     /translation="MYKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKSEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10379..10975
                     /gene="nrfG"
                     /locus_tag="ACHOUK_001514"
     CDS             10379..10975
                     /gene="nrfG"
                     /locus_tag="ACHOUK_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFP9896988.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCIGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQNPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11317..12630
                     /gene="gltP"
                     /locus_tag="ACHOUK_001515"
     CDS             11317..12630
                     /gene="gltP"
                     /locus_tag="ACHOUK_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFP9896989.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12808..13497)
                     /gene="yjcO"
                     /locus_tag="ACHOUK_001516"
     CDS             complement(12808..13497)
                     /gene="yjcO"
                     /locus_tag="ACHOUK_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFP9896990.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13591..15738)
                     /gene="fdhF"
                     /locus_tag="ACHOUK_001517"
     CDS             complement(13591..15738)
                     /gene="fdhF"
                     /locus_tag="ACHOUK_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(15319..15321),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase N subunit alpha,
                     selenocysteine-containing"
                     /protein_id="HFP9896991.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLSIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15936..17402)
                     /gene="mdtP"
                     /locus_tag="ACHOUK_001518"
     CDS             complement(15936..17402)
                     /gene="mdtP"
                     /locus_tag="ACHOUK_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFP9896992.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVTAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGSGYQAAPVVEKK"
     gene            complement(17399..19450)
                     /gene="mdtO"
                     /locus_tag="ACHOUK_001519"
     CDS             complement(17399..19450)
                     /gene="mdtO"
                     /locus_tag="ACHOUK_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFP9896993.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAITEGQCWQSDWRISESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIHQGRDLLHAWDATWNSAQALDNALQPDKAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19450..20481)
                     /gene="mdtN"
                     /locus_tag="ACHOUK_001520"
     CDS             complement(19450..20481)
                     /gene="mdtN"
                     /locus_tag="ACHOUK_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFP9896994.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYV
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKSEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20500..20775)
                     /gene="ytcA"
                     /locus_tag="ACHOUK_001521"
     CDS             complement(20500..20775)
                     /gene="ytcA"
                     /locus_tag="ACHOUK_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFP9896995.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRVIQRANIKLAFVGIIYTALFALYAMLFWLAFF"
     gene            complement(20984..22969)
                     /gene="yjcS"
                     /locus_tag="ACHOUK_001522"
     CDS             complement(20984..22969)
                     /gene="yjcS"
                     /locus_tag="ACHOUK_001522"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFP9896996.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKVYYRADDYKFDINAAAPETV
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTITFIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVKALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMAVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQPQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            23260..23967
                     /locus_tag="ACHOUK_001523"
     CDS             23260..23967
                     /locus_tag="ACHOUK_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9896997.1"
                     /translation="MKPVVLVVDDDTAICALLQDVLSEHVFTVSVCHTGQEAILRIEG
                     DPDIALVVLDMMLPDTNGLRVLQQIQKLRPTLPVVMLTGMGSESDVVVGLEMGADDYI
                     CKPFTPRVVVARLKAVLRRVGALAVNDEKSAGLSFNGWYLDTMRCQLHNPLQQHVELT
                     QGEYGLLLALAQNARRVLSREQLLAFTHSDSVEVFDRTIDVLIMRLRRKIELNPHQPM
                     LIKTLRGLGYVFAADVH"
     gene            complement(23964..24965)
                     /locus_tag="ACHOUK_001524"
     CDS             complement(23964..24965)
                     /locus_tag="ACHOUK_001524"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFP9896998.1"
                     /translation="MMERKGIIAAGNMLVDHVHQIIQWPERGWLAEITHSERSTGGAP
                     LNVLLTLAKMRTGLPLQAVGLIGQDSDGDYIMAMLEQYHVNRQHVQRTTFAPTSMSQV
                     MTDPSGQRTFFHSPGANRLLDLPAFDQLDNTMKIFHLGYLLLLDSLDMPDDVYGTRSA
                     RLLAQMRDTGFETSLDLVSRKGDPRYQPLVLPALRHLDYLVINELEAGEFSGLEIRLP
                     NGEPHIAHIAAAARELLDAGVRQRVVIHYPEGAWGETPEQGGVWIPSQKLEQREIIGS
                     VGAGDAFCAGFLYGCHERWTLTESIKLAHACARASLLCANAIDGAKTLEELQTEMSD"
     gene            complement(24962..25822)
                     /locus_tag="ACHOUK_001525"
     CDS             complement(24962..25822)
                     /locus_tag="ACHOUK_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose 1,6-bisphosphate aldolase"
                     /protein_id="HFP9896999.1"
                     /translation="MPLISLAEGLAHAREHHYSLGAFNVLDSHFLRALFAAARQERSP
                     FIINIAEVHFKYISLDSLVETVKFEAARHDIPVVLNLDHGLHFESVVQALRLGFSSVM
                     FDGSTLGYEENVRQTREVVKMCHAVGVSVEAELGAVGGDEGGALYGHADESLFTDPQL
                     ARDFVDRTGIDALAVAIGNAHGKYRGEPKLDFPRLDAIRQQAAIPLVLHGGSGISDAD
                     FRRAIELGIHKINFYTGMSQAALAAVEARMAHRQPMYDEFAELLLAVEAAISDTVAQQ
                     MRIFGSAGQA"
     gene            complement(25837..26520)
                     /locus_tag="ACHOUK_001526"
     CDS             complement(25837..26520)
                     /locus_tag="ACHOUK_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HFP9897000.1"
                     /translation="MKRSAINDILGHTRQFFSQHDVHLPPFASFSPAQWQQLDTAAWE
                     EVFDLKLGWDVTAFGRNNFAAHGLTLFTLRNGSAKGMPYVKCYAEKIMHVRDAQVTPM
                     HFHWRKREDIINRGGGNLIVELWNADSNEQTADSDITVVIDGCRQKHTAGSQLRLSPG
                     ESICLPPGLYHSFWAEAGFGDVLVGEVSSVNDDDHDNHFLQPLDRYNLIDEDEPAQLV
                     LCNEYRQFR"
     gene            complement(26575..27516)
                     /locus_tag="ACHOUK_001527"
     CDS             complement(26575..27516)
                     /locus_tag="ACHOUK_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9897001.1"
                     /translation="MRLKPLVTALCAGALLAAAPFAQAKELKSIGVTVGDLANPFFVQ
                     ITKGAELEARKLAGDNVKVTLVSSGYDLGQQVSQIDNFIAANVDMIILNAADSKGIGP
                     AVKRAKDAGIVVVAVDVAAEGADATITSDNTQAGEMACKYITDRLKGKGNVVIINGPP
                     VSAVQNRVEGCQTEFKRHPDIKVLSDNQNAKGSREGGLEVMTGLLAANPKIDGVFAIN
                     DPTAIGADLAAKQAQRNEFFIVGVDGSPDAEEALKRENSLFVATPAQDPQVMAAKAVE
                     IGYDILQGKPAPKEPVLIPVTMIDKNNVGNYKGWTVK"
     gene            complement(27535..28509)
                     /locus_tag="ACHOUK_001528"
     CDS             complement(27535..28509)
                     /locus_tag="ACHOUK_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFP9897002.1"
                     /translation="MTTSAKTSAKKMLMSDLMQTVGILPILILIVAVFGFITPNFFTE
                     SNLLNITRQASINIVLAAGMTFIILTGGIDLSVGSILGTTAVAAMVVSLSPEFAMLSI
                     PAALMLGLLLGLFNGALVAFAGLPPFIVTLGTYTALRGAAYLLADGTTVINSNINFEW
                     IGNNYLGPVPWLVVIALAVIVVCWFILRRTTLGVHIYAVGGNMQAARLTGIKVWLVLL
                     FVYGMSGLLSGLGGIMSASRLYSANGNLGMGYELDAIAAVILGGTSFVGGIGTITGTL
                     VGALIIATLNNGMTLMGVSYFWQLVIKGAMIIIAVLIDKYRTRHHQSA"
     gene            complement(28506..30026)
                     /locus_tag="ACHOUK_001529"
     CDS             complement(28506..30026)
                     /locus_tag="ACHOUK_001529"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFP9897003.1"
                     /translation="MNSTPVLEMRNIAKAFGKFYALKGVDLTVYPGEIHALMGENGAG
                     KSTLMKVLAGAYTATSGEILIDGKPFHIRTPKDALSAGITLIYQEMQLAPNLSVAENI
                     FLGSELSHGGLVQRKEMLVQAQKVIDRLGAQFNASDKVMTLTIAEQQQVEIARALHRN
                     SRILVMDEPTAALSSRETHRLFELIMRLRDEGMAIIYISHRMAEVYELSDRVSVLRDG
                     QYVGSLTRDNLNAGELVRMMVGRPLSDLFNKERDIPLGKARLNVHHLTDGGKVQPSSL
                     LVRSGEIVGLAGLVGAGRSELAQLIFGVRKATGGMIEVDGEPVVIHSPREAIDLGIGF
                     LTENRKEQGLFLEMAAAENITMATLERDARWGMLNRKKAQTISDDAIKLLNIRVPHAQ
                     VRAGGLSGGNQQKLLISRWVAIGPRILLLDEPARGVDVGAKSEIYRIMNEMARKGVAI
                     LMISSELPEIVGMSDRVYVMREGSIAGELNGKNITQENIMTLATGVNDAHSQAVTS"
     gene            30140..32410
                     /locus_tag="ACHOUK_001530"
     CDS             30140..32410
                     /locus_tag="ACHOUK_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9897004.1"
                     /translation="MAHPGRHFFASARGRLLLLNLLVVAVTLMVSGVAVMGFRHASQM
                     QELVQQQTVDDMTGSLNLARDTANVATAAVRLSQVVGALEYKGEAERLQETQRALKSS
                     LAQLANAPLAQQEAGLVTRIITRSNELQTSVGGMLERGQRRHLERNALLSSLYQNLSY
                     LRHLQKVTHAQDDILLNEMNRLIVAAIATPAPQAIIHQLVGVMSALPTHSDTPLVNTL
                     LNDFNDFNDEMRKLAPLSAALEQSDLAISWYMFHIKALVAILNGDINRYAEQVATLSG
                     QRVAQSYQDLRSGERVIMIFALLAVVITAFAGWYIYRNLGTNLTAISRAMSRLAHGEA
                     DVTFPALQRRDELGELARAFNVFARNTASLERTSRLLKEKTTQMEIAQTERQGLEEAL
                     LHSQKLKAVGQLTGGLAHDFNNLLAVIIGSLELVNPDSPDAPRIQRALQAAERGALLT
                     QRLLAFSRKQSLHPQAVELKTLLEELSELMRHSLPATLALEIEAQSPAWPAWIDISQL
                     ENAIINLVMNARDAMEGQTGIIKIRTWNQRVTRSSGQRQDMVALEVIDHGCGMSQAVK
                     SQVFEPFFTTKQTGSGSGLGLSMVYGFVRQSGGRVEIESAPGQGTTVRLQLPRALTAA
                     KNLSPAAVEQASVSGDKLVLVLEDEAAVRQTICEQLHLLGYLTLEASSGEQALDLLAA
                     SAEIDIFISDLMLPGGMSGAEVVNAARKLYPHLTLLLISGQDLRPSHNPALPDVALLR
                     KPFTRAQLAQALGQEN"
     gene            32479..32808
                     /gene="yjdP"
                     /locus_tag="ACHOUK_001531"
     CDS             32479..32808
                     /gene="yjdP"
                     /locus_tag="ACHOUK_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFP9897005.1"
                     /translation="MKRHSTLFLFTLLTLTTVPAQADIIDDTIGNIQQAINDASNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQHQLNQERRQLEDEERR
                     MEDEYGQ"
     gene            complement(32885..33643)
                     /gene="phnP"
                     /locus_tag="ACHOUK_001532"
     CDS             complement(32885..33643)
                     /gene="phnP"
                     /locus_tag="ACHOUK_001532"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFP9897006.1"
                     /translation="MSLTLTLTGSGGAQGVPAWGCECAACARARRSPHYRRQPCSGVV
                     KFNEAITLIDAGRHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRNHCDLNTVLALNQVIRSPRV
                     VLTHISHQFDAWLMENALPSGFEAGFDGMEIGVA"
     gene            complement(33645..34080)
                     /gene="phnO"
                     /locus_tag="ACHOUK_001533"
                     /pseudo
     CDS             complement(33645..34080)
                     /gene="phnO"
                     /locus_tag="ACHOUK_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
     gene            complement(34067..34624)
                     /gene="phnN"
                     /locus_tag="ACHOUK_001534"
     CDS             complement(34067..34624)
                     /gene="phnN"
                     /locus_tag="ACHOUK_001534"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFP9897007.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPICLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHHKEKHHACL"
     gene            complement(34624..35760)
                     /gene="phnM"
                     /locus_tag="ACHOUK_001535"
     CDS             complement(34624..35760)
                     /gene="phnM"
                     /locus_tag="ACHOUK_001535"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFP9897008.1"
                     /translation="MIINNVKLVLENEVVNGSLEMQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHNTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAM
                     CRARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDQSNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            complement(35757..36437)
                     /gene="phnL"
                     /locus_tag="ACHOUK_001536"
     CDS             complement(35757..36437)
                     /gene="phnL"
                     /locus_tag="ACHOUK_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFP9897009.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWRLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            complement(36548..37306)
                     /gene="phnK"
                     /locus_tag="ACHOUK_001537"
     CDS             complement(36548..37306)
                     /gene="phnK"
                     /locus_tag="ACHOUK_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFP9897010.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYGMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            complement(37303..38148)
                     /gene="phnJ"
                     /locus_tag="ACHOUK_001538"
     CDS             complement(37303..38148)
                     /gene="phnJ"
                     /locus_tag="ACHOUK_001538"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFP9897011.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     VIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            complement(38141..39205)
                     /gene="phnI"
                     /locus_tag="ACHOUK_001539"
     CDS             complement(38141..39205)
                     /gene="phnI"
                     /locus_tag="ACHOUK_001539"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFP9897012.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDESPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            complement(39205..39789)
                     /gene="phnH"
                     /locus_tag="ACHOUK_001540"
     CDS             complement(39205..39789)
                     /gene="phnH"
                     /locus_tag="ACHOUK_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFP9897013.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVSQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAVPRTTHVEVC"
     gene            complement(39786..40238)
                     /gene="phnG"
                     /locus_tag="ACHOUK_001541"
     CDS             complement(39786..40238)
                     /gene="phnG"
                     /locus_tag="ACHOUK_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFP9897014.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITANYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQTRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            complement(40239..40964)
                     /gene="phnF"
                     /locus_tag="ACHOUK_001542"
     CDS             complement(40239..40964)
                     /gene="phnF"
                     /locus_tag="ACHOUK_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFP9897015.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGIALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            complement(40985..41764)
                     /gene="phnE"
                     /locus_tag="ACHOUK_001543"
     CDS             complement(40985..41764)
                     /gene="phnE"
                     /locus_tag="ACHOUK_001543"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFP9897016.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMLVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            complement(41870..42886)
                     /gene="phnD"
                     /locus_tag="ACHOUK_001544"
     CDS             complement(41870..42886)
                     /gene="phnD"
                     /locus_tag="ACHOUK_001544"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9897017.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFSKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAMQ"
     gene            complement(42911..43699)
                     /gene="phnC"
                     /locus_tag="ACHOUK_001545"
     CDS             complement(42911..43699)
                     /gene="phnC"
                     /locus_tag="ACHOUK_001545"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFP9897018.1"
                     /translation="MQTIICVEQLSKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMAHFAYQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRVEENAKAA"
     gene            complement(43832..44275)
                     /gene="yjdN"
                     /locus_tag="ACHOUK_001546"
     CDS             complement(43832..44275)
                     /gene="yjdN"
                     /locus_tag="ACHOUK_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFP9897019.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            complement(44534..44869)
                     /gene="yjdM"
                     /locus_tag="ACHOUK_001547"
     CDS             complement(44534..44869)
                     /gene="yjdM"
                     /locus_tag="ACHOUK_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFP9897020.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
BASE COUNT         9704 a        12277 c        12741 g        10356 t
ORIGIN      
        1 gtttatttgt ttaatattat gggaaaaggt gatgcatttg ggagaggaag agtattcccc
       61 ggtcagacga ccggggaagg ggagagatta atgcgcgcgg ccttgctcaa cgccaaagcc
      121 ggtctgggag cggataaact gcgcgcggaa cagttcacgc tcacgcgcgc cttccgctga
      181 gttatcggtt gccgagaaga accagatgcc gaggaatgcc acggtgatag agaacagcgc
      241 cgggtattca tacgggaaga tggctttttc gtgaccgagg atctgtaccc aaatcgtcgg
      301 gccgaggatc atcagtacca ctgcggtaat cagccccagc cagccaccca tcatcgcgcc
      361 acgcgtggtc agtttcgacc agtacataga aagcagaatg atcgggaagt tacagctcgc
      421 cgcgatggca aacgccagcc ccaccataaa ggcgatgttc tgattctcaa acagcacgcc
      481 gaggataatc gcaatcacac ccaaaatcag gacggtgatt ttcgataccc gcagctcttc
      541 acgttcggtc gcgccttttt tgaagacgtt agcgtacagg tcatgcgaaa ccgctgatgc
      601 gcccgccagc gtcagacccg caaccaccgc gaggatagtg gcgaaagcaa ccgctgaaat
      661 aaagccgagg aacaggttgc cgcccaccgc attggccagg tgaactgctg ccatgttgtt
      721 accaccaatc agatggcccg ccgcgtcttt atattccgga ttcgcaccga ccagcatgat
      781 cgcgccgaag ccgataataa aggtcaggat atagaagtag cccataaatc cggtagcgta
      841 gaacacgctt ttacgcgctt cgcgggcatc gctgaccgta aagaagcgca tcagaatgtg
      901 cggcaagcca gccgtaccaa acatcagtcc tagccccaga gagagtgcgg agatcggatc
      961 tttcaccagt ccgcccggtt tcatgatgtc gacacctttc gggtgtaccg ccatcgcttc
     1021 gctgaacaga ttgttgaagc taaagccgac gtgtttcatc accataaagg ccataaagct
     1081 ggcaccgaac agcaacagca cagctttgat aatctgtacc caggtggtcg ccagcatgcc
     1141 gccgaacagg acgtacatca tcatcagcac gccgaccagc accactgcaa tgtgatagtt
     1201 aaggccaaac agcagctcga tcagtttacc tgcacccacc atctgggcga taaggtaaag
     1261 cgccaccacc accagagaac cacaggccga aagaatacga atcggccctt gtttcagacg
     1321 ataagaggct acatcggcaa aggtgtaacg ccccaggtta cgcagacgtt cggcaatcag
     1381 gaacaaaatg atcggccagc ccaccaggaa gcccagcgag taaattaagc cgtcatagcc
     1441 ggaggtaaac accagtgcgg agatccccaa aaatgaggcg gcggacatat agtcaccggc
     1501 aatcgccagc ccgttctgaa aaccggtgat attgccgcct gcggtgtaat agtcattacg
     1561 cgaacgtacg cgttttgacg cccagtaggt aatgccgagc gtaaacacga cgaaaatcag
     1621 gaacataata atcgcctgcc agttcgttgg ctggcgctct acggccccgc taatagcatc
     1681 cgcggcgtta gctgcgaaag ggagtgtggc ggcaagcgcc gtcagaactc ttttcatgat
     1741 gcttgtacct catgcaggac ttcattatta agacggtcga attcgccgtt cgcccgccag
     1801 atgtagatac cagtgagaac aaaggagatc actattaccc caacaccgac tggaataccg
     1861 cgtgtgacgc tggtgttcgg attcagcggg gtgcccagcc agccgggcgc gaaggcgatc
     1921 agtaaaataa agccgatata aactgccagc ataataatcg acaggatggt ggcaaaccgt
     1981 tgccgttttt cgactaactc cctgaaatgc gcattgtctt ctatccgctg ataaatagtg
     2041 ccattcatca cagattctcc agaggtaatg tagggattgt tttaattccc gctcccttat
     2101 gggagaaggt taacgctcgg gtaacccttg ccgaatgtag gccggataag gcgttatacg
     2161 ccgcatccgg caatcaatgc ctgatgcgac gctgtcgcgt cttatcaggc ctacaaaccg
     2221 ttaccgactc gcatccggca attgtgggtt acgatggcat cgcgatagcc tgcttctctt
     2281 caagcagctt ctcgactacg ccaggatcgg caagcgtcga ggtatcgccc aggttgctgg
     2341 tatcgcccgc cgcaattttg cgcagaatac ggcgcataat tttgccggag cgggttttag
     2401 gcagggagtc ggtccagtgc agcacgtctg gcgtcgccag cgggccaatc tctttacgca
     2461 cccagttgcg gacttctgcg tacagttctg gtgacggttc ctccccgtga ttaagcgtga
     2521 cgtaggcgta gatcgcctga cctttaatat tgtgcggaat accgacgaca gcggcttcgg
     2581 cgattttcgg atgcgccacc agcgccgact caatctccgc cgttcccaga cggtgaccgg
     2641 agacgttcag cacatcgtcc acacgcccgg tgatccagta atagctatct tcatcacgac
     2701 gcgcgccgtc gccgctgaaa tacatatttt tgaaggtgga aaaataggtc tgctcaaaac
     2761 gttcgtgatc gccaaacagc gtacgcgcct gacccggcca ggagtcggtg atcaccaggc
     2821 taccttcggt agccccttcc agcgggttac cttcgttatc gaccagcgcc ggttgcacgc
     2881 cgaagaacgg acgtgttgcc gaaccggctt tcagctcggt agcgccaggc agcggggtga
     2941 tcatgaaacc gccggtttcg gtctgccacc aggtatcgac caccggacat ttctcgttgc
     3001 cgattttttt ccagtaccac tcccacgctt ccgggttaat tggctcgccc acggaaccga
     3061 gaatgcgcag cgacgaacgg tcggtgcctt cgatcgcttt atcgccttcc gccatcagcg
     3121 cgcgaatcgc cgtgggcgcg gtatagagaa tattgacctg atgcttgtcc accacctgtg
     3181 ccatacgggc aggcgtcggc cagttcggta cgccttcaaa catcagcgtg gtcgcgccgc
     3241 aggccagcgg gccgtacagc aaataactgt gtccggtcac ccagcccaca tcggcggtgc
     3301 accagtagat atcgcccgga tgataatcaa agacatattt aaaggtcagc gccgcgtaca
     3361 ccagataacc gccggtagtg tgcagtacgc cttttggttt tccggtagaa ccggaggtat
     3421 agagaataaa cagcggatct tcggcgttca tctcttccgc ctggtgctga tcgctggctt
     3481 gctcaacctg gtcgtgccac cacaggtcgc gcccttcctg ccagtcaatt ttcccgccag
     3541 tacgcttcag taccaccaca tgctctacgc tggtgacgtt cgggtttttt agtgcgtcat
     3601 caacgttttt cttcagcgga atactacgcc cggcgcgcac gccttcgtcg gaagtgatca
     3661 ccagtcgtga gttggaatcg ataatgcgcc cggcaaccgc ttccggcgag aagccaccga
     3721 aaattaccga atgcaccgcg ccaatacggg cgcaggccag catcgcaacc gcggcttccg
     3781 gcaccatcgg catataaatc gccaccacat cacctttttt aatgcccagc ttgagcaggg
     3841 tattggcgaa gcggcagacg tcgcggtgca gctctttata gctgatatgt ttgctctggc
     3901 tggcgtcgtc gccttcccag atgatggcgg tacgatcgcc gttttcttgc agatggcggt
     3961 caaggcagtt tgccgccaga ttcagcgtgc cgtcctcgta ccatttaatg gacacattac
     4021 cgggggcaaa ggaggtgttt ttcaccttct ggtacggttt gatccagtcg agaatttttc
     4081 cctgttcgcc ccagaaggta tcaggtgcgt taatagattg ttgatacatc gcctcgtact
     4141 gctgagggtt tatcaggcaa cggtctgcga tgttggcagg aatggtgtgt ttgtgaattt
     4201 gactcatgct tttgttctcc ttgtaggatg ttaataatat gtggcataag cgttaaatgt
     4261 aggggtattg gcagttttgt ttagtatttg ggcgacagat cacgcaaaag tagaattgtg
     4321 caaataaacg gcagggtaat ctttgaaggt cagcaacaaa agttgattaa ttctttgagg
     4381 aacatgcagt tatgcatgct gttgaaaaag aggaagatac tgactaactc taaagtggta
     4441 ttttacatgc acttacaatt gattaaagac gacattcaca gtgtggttat ttgttacact
     4501 taggggcgag caatgtcatg acagtgtagg tgcggttact gtcgtgaaaa acaataaaaa
     4561 ccgccattgc aacaatggcg caattcggat gaagccccta tgacaaggat aaaaataaac
     4621 gcacgccgta tcttcagctt attgattcct tttttctttt tcacttctgt tcacgctgaa
     4681 caaacggctg ctcccgcaaa acctgtaact gtggaagcga agaatgaaac ctttgccccg
     4741 cagcatcccg atcaatatct ctcctggaaa gccacctcgg aacagtcaga gcgtgttgac
     4801 gccctggcgg aagatccacg gctggtgatc ctgtgggcgg ggtatccctt ctcgcgcgat
     4861 tacaacaagc cgcgtggaca tgcctttgct gtgaccgatg tgcgtgaaac cctgcgtacc
     4921 ggtgcgccga aaaacgctga agatggtccg ctaccgatgg cgtgctggag ttgtaaaagc
     4981 ccggatgtgg cgcgtctgat ccagaaagac ggcgaagatg gctacttcca cggtaaatgg
     5041 gcgcgcggcg gcccggaaat cgtcaacaac ttaggttgtg ccgattgcca taacaccgcc
     5101 tctccagagt tcgccaaagg caaaccggag ttaacccttt cccgtccgta tgcggctcgc
     5161 gcgatggaag ccattggtaa accttttgag aaagccggac gtttcgacca gcaatcgatg
     5221 gtttgcggtc agtgccatgt ggagtattac ttcgacggca aaaacaaagc ggttaaattc
     5281 ccgtgggatg acggcatgaa agtcgaaaat atggagcagt attacgacaa aattgccttc
     5341 tctgactgga ctaactccct gtcgaaaacg ccaatgctga aagcgcagca cccggaatat
     5401 gaaacctgga cagcgggcat tcacggtaaa aacaacgtga cctgtatcga ctgccatatg
     5461 ccaaaagtgc agaacgccga aggcaaactc tacaccgacc ataaaattgg taatccgttt
     5521 gataacttcg cccagacttg tgcgaactgc catacccagg acaaagctgc cttgcaaaaa
     5581 gtggtcgcgg aacgtaagca gtcgattaac gacctgaaaa tcaaggttga agatcaactg
     5641 gttcacgctc acttcgaagc gaaagcggcg ctggacgcag gtgcgacgga agccgaaatg
     5701 aagccgattc aggacgatat ccgtcatgcc cagtggcgtt gggatctggc gatcgcttcc
     5761 cacggcattc atatgcacgc accggaagaa ggtctgagga tgctcggtac ggcgatggat
     5821 aaagcggcgg atgcacgcac caaactggcg cgcctgctgg cgactaaagg catcacccat
     5881 gaaatccaga tcccggatat ctcgaccaaa gagaaagccc agcaggccat tggcctgaac
     5941 atggaacaaa tcaaggccga gaagcaggac ttcatcaaaa cggtgatccc gcagtgggaa
     6001 gagcaggcac gtaaaaacgg tctgttaagc caataacccc gttccgcctc gcaaggggcg
     6061 gaaaacacaa tggagtgaat atgagcgtat tacgttcgtt attaactgcc ggggtgctgg
     6121 cgtcgggcct gttgtggagc ctgaacggaa ttaccgctac ccctgcggcg caggcatctg
     6181 atgatcgcta cgaagttacc cagcagcgta acccggatgc cgcctgtctg gactgtcata
     6241 agccagatac cgaaggtatg catggcaaac atgcttccgt tatcaacccg aataacaaac
     6301 tgccggtcac ctgcaccaac tgtcacggcc agccatcacc gcaacaccgc gaaggggtga
     6361 aagatgtgat gcgctttaac gagccgatgt acaaggttgg agagcagaac agcgtctgta
     6421 tgtcctgcca tctgccggaa cagttgcaaa aagcgttctg gccgcacgat gtccacgtaa
     6481 ccaaagtggc gtgcgccagc tgccattccc tgcatccaca gcaagatacg atgcagacgt
     6541 taagtgacaa aggacggatt aagatttgcg tcgattgcca cagcgatcag cgcaccaatc
     6601 cgaactttaa cccggcgtct gttccgttgc ttaaggagca gccatgacct ggtctcgtcg
     6661 ccagtttctc accggcgtcg gcgtgctggc agccgtcagc ggcaccgcag ggcgcgtggt
     6721 ggcgaaaacg ttgaatatca atggggtgcg ttacggcatg gtgcatgacg agtcgttatg
     6781 catcggctgt accgcctgta tggatgcttg tcgggaagtg aacaaggtgc cggaaggcgt
     6841 ctcgcgcctg acgattattc gtagcgagcc gcagggcgaa tttcctgatg tgaaatatcg
     6901 cttcttccgt aagtcttgcc agcactgcga tcatgcgccg tgcgttgacg tctgcccgac
     6961 cggtgcatct tttcgcgatg ctgccagtgg gattgtcgat gttaatccgg atctctgcgt
     7021 cggttgtcag tactgcatcg ccgcctgtcc gtaccgcgtg cgctttattc atccggtcac
     7081 gaagacagcg gacaaatgcg atttctgccg taagaccaat ttgcaggccg gtaagcttcc
     7141 cgcgtgcgtt gaggcttgcc cgacgaaggc gctgacgttt ggcaatctgg acgatcccaa
     7201 cagtgagatt tcgcaactgc tgcgccagaa gcccacttac cgctacaagc tggcgctggg
     7261 aaccaaaccg aagctgtacc gcgtaccgtt taaatacggg gaggtgagcc aatgacgcag
     7321 acttccgcat ttcattttga atcgctggtg tgggactggc cgattgccat ctacctgttt
     7381 ttgattggta tttctgccgg tctggtgacg ctggccgtgc tgttacgtcg cttctacccg
     7441 caggcgggcg gtgcagacag cacgttgctg cgcaccacgc tgattgtcgg acctggcgcg
     7501 gtgatcctcg gtctgttgag cctcgtcttc cacctgacaa gaccgtggac cttctggaag
     7561 ctgatgttcc actacagttt tacttcggtg atgtcgatgg gggtgatgct gtttcagctt
     7621 tacatggtgg tgctggtgct gtggctggcg aaaatctttg aacatgattt gcttgccctg
     7681 caacaacgct ggttgccgaa gctggggatc gtgcaaaagg ttctgagcct gctgacgccc
     7741 gttcatcgcg gactggaaac attgatgctg gtgctggcgg tgttgttggg ggcttatacc
     7801 ggttttctgc tgtcggcgct gaaatcgtat ccgttcctta ataacccgat cctgccggtg
     7861 ctgttcctct tctccggcat ctcgtccggt gcggcggtgg cactgatcgc catggcgata
     7921 cgccaacgca gtaacccgca ttccacggaa gcgcagtttg tacaccgtat ggaaatcccc
     7981 gtggtatggg gtgaaatctt cctgctggcg gcgttttttg tcggtctggc gctgggcgat
     8041 gacggtaaag tgcgtgcgct ggtggcggca ttgggcggcg gtttctggac gtggtggttc
     8101 tggcttggtg tcgccgggct ggggctgatt gtgccaatgt tgttcaaacc gtgggtcaat
     8161 cgcagttccg gcattcctgc cgtgctggcg gcgtgtgggg ccagtctggt cggcgtgttg
     8221 atgttgcgat ttttcattct ctacgccggg cagttaacgg tggcgtaagc cagaacagag
     8281 gtggtttctg gacgtattcc ttcctgaagt cggttttctg gcgctgttgt taagtctcgg
     8341 ggtcaacgtg ttgaccccgt tgacggcctt tgcgggagtg cggttgcgct ggcctgccat
     8401 gatgcgactc acttgcatcg gcattctggc acagttcgcg ctcctgctgc tcgcctttgg
     8461 cgtactgacg tattgttttc tcatcagcga tttctcggtc atttatgtcg cccaacatag
     8521 ctacagcttg ctgtcgtgga aactcaaact ggcggcggtg tggggcggtc atgaaggttc
     8581 gctgctgctt tgggtgctgc tgctttccgc ctggagcgcg ctgtttgcct ggcaatatcg
     8641 gcagcaaacc gatccgctat ttccgctgac gctagccgtt ttatctctca tgctcgccgc
     8701 actgctactg tttgtagtgc tgtggtccga tcccttcgtg cggatatttc caccagcaat
     8761 cgaaggccgc gatctcaatc cgatgctgca acatcccggt cttatctttc atccaccgct
     8821 gctttacctt ggctatggcg gtttgatggt agcggcgagc gtggcgctgg cgagtctact
     8881 gcgcggcgag tttgatggtg cctgcgcccg aatttgctgg cgctgggcgt tacctggctg
     8941 gagtgcatta actgcgggga tcatcctcgg ttcctggtgg gcctactgcg aactcggctg
     9001 gggcggctgg tggttctggg atccggtgga aaacgcctct ttattaccct ggctttctgc
     9061 cactgcgctg ctgcacagtt tatccctgac acgccagcgg gggatttttc gccactggtc
     9121 gctgttgttg gcgatagtta ctctgatgct gtcgctgctg ggcaccttaa ttgtccgttc
     9181 tggcattctg gtttcggttc atgcgttcgc tctggataac gttcgcgctg tgccgttgtt
     9241 cagcctgttt gcactgatta gccttgcgtc tctggctctg tatggctggc gagcgcggga
     9301 cggtggcccg gcggtgcgtt tttcggggtt atcgcgggaa atgttaatcc tcgctacgct
     9361 gttgctgttt tgcgcagtgc tactgatcgt gctggtggga acgctttatc cgatgattta
     9421 cggcctgctg ggctggggac gtctctccgt tggcgcaccg tattttaacc gcgcgacgtt
     9481 accgtttggt ctgttgatgt tggtggtgat tgtgctggcg acgtttgtct ctggcaaacg
     9541 cgcgcagctt ccggcgctgc tggcgcatgc gggcatactg ttatttgccg cagggatcgt
     9601 tgtctccagc gtcagccgtc aggagatcag cctgaattta cagccgggtc agcaggtgac
     9661 gctggcagga tacaccttcc gttttgagcg cctcgatctg caagccaaag gcaattacac
     9721 cagcgaaaaa gcgatagtgg cgctgtttga ccatcagcaa cgcattggtg aattaacgcc
     9781 ggagcggcgt ttttacgaag ctcgtcgtca gcaaatgatg gaaccgtcaa ttcgctggaa
     9841 cggcatccat gactggtatg cggtcatggg ggagaaaact ggagcggatc gttacgcttt
     9901 tcgcttgtat gtacaaagcg gtgtgcgctg gatctggggg ggaggattgt tgatgattgc
     9961 gggcgcattg ttaagcggat ggcgggggaa gaagcgcgat gtataaaggg cttctcacgc
    10021 tactgctgtt atttacctgt tttgcgcgcg ctcaggttgt agacacctgg caattcgcca
    10081 atccgcaaca acagcaacag gcgttaaata ttgccagcca gttacgttgt ccgcagtgcc
    10141 agaatcaaaa cttactggaa tccaacgcgc cggtggcagt cagtatgcgc catcaggttt
    10201 acagcatggt ggcggagggg aaaagtgaag tcgaaatcat tggctggatg accgaacgct
    10261 acggagattt tgttcgctat aacccaccgt taacgggtca gacgctggtg ttgtgggcgc
    10321 tgccagtggt gttgttactg ctgatggcac tgatcctctg gcgagtgagg gcgaagcgat
    10381 gaaacagcct caaataccgg tgaaaatgct gacaacactc acgatattga tggtattcct
    10441 ctgtatcggt agttatctgt taagcccaaa atggcaggcg gtacgtgcgg agtatcagcg
    10501 tcagcgcgat ccgctacatc agtttgccag ccagcaaaac ccggaagcgc agcttcaggc
    10561 attgcaggat aaaatccgcg ctaatccaca aaacagcgaa cagtgggcgt tactgggcga
    10621 gtactatctg tggcaaaacg attacagcaa ttcgctgctg gcgtaccgtc aggcgttgca
    10681 actacgtggt gagaacgctg aactgtatgc ggcgctggcg acggtgcttt attaccaggc
    10741 cagccagcat atgaccgccc agactcgcgc aatgatcgac aaagccctcg cgctggactc
    10801 taacgaaatc accgctctga tgctgctggc ttccgatgcg tttatgcagg cgaattacgc
    10861 gcaagccatc gaactatggc aaaaagtgat ggatctcaac tcaccccgga tcaaccggac
    10921 acaactggtc gagtcgatta atatggcgaa attgttgcag cggagatcgg attaatcatc
    10981 tggtgatgaa gattatgtta ttgagctgca taaaaaataa tcgaatgaac atatgccaaa
    11041 aataatcact aatcagtatt attgcagatt aaacaaataa aaatctttcc ataacaaatg
    11101 gttattcatt aatcctgcca taaactcgcg ttattatgca ttaatgcagc gaaaagctct
    11161 gttgttaaag ggttgcgcaa cataccgcgc aaatgatact gatcataagc gttaaaaaaa
    11221 tctacaaacc aacgcaacac aattcatgcc ctggcagtat gtcacgttct cgcgtttctg
    11281 aacggggaac ggcgctccat tgaggaagtc attcatatga aaaatataaa attcagcctg
    11341 gcctggcaga ttctgtttgc tatggtgctg ggcattctcc tgggaagcta cctgcactac
    11401 catagcgaca gccgcgactg gctggtcgtc aatttgctct ctccggcggg tgatatcttc
    11461 atccatctga tcaaaatgat tgttgtgccg attgtgatct ccacgctggt ggtgggtatc
    11521 gcgggtgttg gtgatgccaa acagctcggg cgtattggcg cgaaaaccat tatctacttc
    11581 gaggtgatca ccaccgttgc catcattttg gggatcactc tggcgaacgt cttccagccc
    11641 ggtgccgggg tggatatgtc gcagctggcg accgtcgata tctcgaaata tcagagcact
    11701 acggaagcgg tacaaagcag ttcccacggc attatgggca cgattttgtc gctggtgccg
    11761 acgaatattg tggcgtcgat ggcgaaaggc gaaatgttgc cgatcatctt cttctcggtg
    11821 ctgtttggtc tggggctttc ctccctgccc gcgacgcatc gtgaaccgct ggtgaccgtg
    11881 ttccgctcca tctctgaaac catgtttaaa gtgactcaca tggtgatgcg ttatgcgccg
    11941 gtgggtgtgt ttgcgctgat tgcagtgacg gtggctaact ttggtttctc ttctctgtgg
    12001 ccactggcga aactggtgct gctggtgcat ttcgccattc tgttcttcgc gctggtagtg
    12061 ctgggaattg tggcgcgcct atgcgggtta agcgtctgga tcctgattcg tattctgaaa
    12121 gatgagctga ttctggcgta ctccactgcc agctctgaaa gcgtactgcc gcgaattatt
    12181 gagaagatgg aagcctacgg agcaccggcg tcgatcacca gcttcgtggt gccgaccggt
    12241 tactctttta accttgatgg ttcgacgctg tatcaaagta ttgccgctat tttcatcgct
    12301 cagctgtatg gcattgacct gtccatctgg caggaaatca ttctggtgct gacgctgatg
    12361 gtgacctcga aagggattgc tggcgtgccg ggcgtgtcgt ttgtggtgtt gctggcaacg
    12421 ctgggtagcg taggtattcc gctggaaggt ctggcgttta ttgctggtgt tgaccgtatc
    12481 ctcgacatgg cgcgtactgc actgaacgtg gtgggtaatg cgctggcggt gctggtgatt
    12541 gccaagtggg aacacaaatt tgaccgcaaa aaggcgctgg cttatgagcg tgaagtgctg
    12601 ggcaaatttg ataaaactgc ggatcaataa ttgaagattg ccggggatat cccccggcaa
    12661 tgtgtggatg cctgatgcga cgctggtgcg tcttatcagg cctacgtcag acagcgctag
    12721 cgtgattttg taggtctgat aaggcgttta cgctgcatcc gacatcaacg cctgatgcga
    12781 cgcttaacgc gtcttatcag gcctacctta accgttagtt aatttttcaa actcttcaca
    12841 cccggtatca aacccttcca tacagctcag gttcaaccag tgcagcgcct tttgcttgtt
    12901 cttctcgata aaaccttctt caccgtttaa gaacatcatt cccgcccagt actcggaata
    12961 accggttcgg gaaattgcag agctgcgttt gaaataccag gttgccttgt catcgtcggc
    13021 cttaatgccc acgccgttgg cgtaaatcaa accaagcagc atttgggcat cgacggcaga
    13081 gtcgttctcc agatcttccg aggcgttttc taacagcgaa atcgcctttg gataatccgg
    13141 tttacccgct tgagtattta ccagaatatg cgccagcgtt acttcacctt ctttactgcc
    13201 cgcttgcgcc gctttctctg caagaacttt tgcttgtgga tagtccagac tgaccggatt
    13261 ggtgattttg atctgcgcca acagcgcgca ggcatcagca tcaccgctgt cggcggcttt
    13321 ctgtgcccaa tattcggctt tgctcaaatc gccggagcta aaccagctgt cggcaagaaa
    13381 atattgtgcg cgtcggtccc cggcctctgc tgcctttaaa tactggctgc caggctcgga
    13441 gtcgttggcg tgggcaaaga atgtcaaaaa caacatcaat gcgataattt ttttcatttt
    13501 gataatacga ttgggtaggg agtaaccagt ataaagagtt cgaatggata aaaaaacagc
    13561 ctccgaaagg aggctgtaga aaggactgta ttacgccagt gccgcttcgc gcaggcgagt
    13621 tttcaacttg ttgtactcgt caatcacgta ctgctcggcg gcgcgctgat cggcgatcgg
    13681 ctcgacgcgc acggcgcagt atttgtattc cggcgttttc gtaatcgggc ttaagttttc
    13741 ggtaaccagc tcgttacagg caccaatcca ccactggtag gtcatgtaaa tcgccccttt
    13801 gttcggacga tcgctgacct gcgcacgggt gataattttg cctttacgcg agtgcaccca
    13861 aaccaatgcc tcatcttcaa tactcagacg tttggcgtct tcggtattga tttgtgcgta
    13921 gccaggttca tcagccagcg ccgccagtgc cgcacagtta ccggtcatcg aacggcaaga
    13981 gtagtgacca acttcacgca ccgttgacag taccatcggg tactcgtcgg tgagtttgtc
    14041 gattggcgct acccagtcgc aggtgaagaa ctgcgccaga ccgttcgggg tgtcaaactt
    14101 ctctttaaac agataagaag tcccctgatc ggcatctgaa gtatcgcggc aaggccactg
    14161 aatgaagccc agttcgccca ttttctcgta agtcgcaccg tagaaatccg ggcacagatg
    14221 acgcaactca tcccagatct cctgggtgtt gttgtagtgc atcggataac ccatacgggt
    14281 ggcgatttca ctgatgattt gccagtccgt tttcagatcc catttcggtt caactgcttt
    14341 gaagaaacgc tggaagccac ggtcagccgc agtaaacacg ccttcatgct cgccccacga
    14401 cgttgacggt aaaataacat ccgccgccga cgcggttttg gtcataaaga tgtcctgaac
    14461 gataaccagt tccagatctt caaaggcttt acgtactgcc gacagctccg cgtcagtttg
    14521 tagcggatct tcgcccataa tgtacgcggc acgcacttcg ccatgcgctg cgcggtgcgg
    14581 cagctcgctg atgcgataac cggtatgagc aggcaggctt tccacgcccc aggctttggc
    14641 gaatttctcg cggttagccg gatcttttac gtactggtat cccggatacg tatccggcag
    14701 cgcgcccata tcgcaggcac cctgcacgtt gttctgacca cgaaccgggt taacacccgc
    14761 atgtggctta ccgaggttac cggtcagcat cgcgaggctg gtcagagaac gcacggtttc
    14821 cacgccctgg tagaactggg ttacacccat gccccacagg atggcggcgc ttttcgcctg
    14881 ggcatacatc cgtgccgcct gacgaatctc actggcgctg acgccggtga tatcttcaac
    14941 cgactccggc gtgtagcctt caacgatttt acgatactct tcaaagcctt ctgtacggga
    15001 agcgacgaat gctttgtcgt acagattttc ttcaataatg acatggccca tcgcattcaa
    15061 cagcgcgatg ttcgagccgt ttttcagtgc aatgtgcatg tcagcaatgc gcgcggtttc
    15121 aattttgcgc ggatcgcaga cgataatttt cgccccgtta cgtttagcgt taattacgtg
    15181 attcgccacg attgggtggg aatccgccgg gttgtacccg aaaacgaaca ctaaatcggt
    15241 attatcaatt tcgttaatag cattgctcat tgcgccatta ccgaccgatt ggtgcagacc
    15301 tgcaaccgat gggccgtgtc agacacgagc gcagcagtca acgttattgg taccaataac
    15361 ggcgcgcgca aatttttgca ttacatagtt ggtttcgtta cccgtaccac gcgaggagcc
    15421 ggtcgtctgg atggcatccg gaccgtactt ctctttgatg gcgctcaggc gctcggcaac
    15481 gtaattcagt gcctcatccc aggaaacagg ttcgagtttg ccgccacgct ggcgacggat
    15541 catgggggtt ttcaggcgcg gggtcaggat ctgggtatcg ttaatgaagt cccagccata
    15601 ataacccttc agacacaggg taccctggtt ggttttcccc tgcgctgcct ccgcccggac
    15661 gattttgccg ttatcgacga ccaggttgat tttgcaacct gatgcgcaat aggggcaaac
    15721 cgtgacgact tttttcatcg gtctcgctcc agttaatcaa atcacgcata cgcgctctcg
    15781 actacagtat gcatctttta tgccacattt tatttggggt cattccctga tattacgggc
    15841 actatttatt caaaactctg acgaaaaaca ggctgtcgtc agttttgacg tgacgaaacg
    15901 aaataccgcg tgacagccat cacacggcag acattttatt ttttctcgac gacgggagcc
    15961 gcctgatacc cgctgcccag cgatttcatc aactgaatgc tttggatcac ccggcggctg
    16021 tccagcatca gtaatgacat ctcttcggca agcaccggca accgggcttc ggtagcctgt
    16081 aagcggctgg ttaagccgcg ctgataggcg gcctcggcag cgcgctgggt gaagcgcgtt
    16141 gcttccacgc gttcagcctg catttctcgc tcgtcgttga gcgtttgcag acgcgtgccg
    16201 ttgacggcaa cgtcacgcac cgcgttcagt actgactggt tgtaacgttc aatcatcatg
    16261 ttgctggcgg cgcgagtgcc ttcgagattg gcattcaacc gtccaccgtc aaacagcggc
    16321 aatttcagac ccgggatgaa gttgaactgg cgactggttt ttttgaataa ggtatccagg
    16381 tggatggagt ccagaccgaa aaacgctttg atatcaaagc ttgggtagaa cagcgcccgc
    16441 gcggaatcca cctgatctaa tgacgcctga acataccagc gcatggcttg cagatccggg
    16501 cgtctggcga gcaactcata agagagtgtc gccggaatac cggtctggac tcgtggtaat
    16561 gccactggtt tgatctccgg catatcgctg gctcccgcgc caatcaatgc acgcagagat
    16621 tctcgcgttt cggtgatttg ccctttaacg gcagcaattt gtttatcgac cgccagaatc
    16681 tgtgcccgcg cgccgtggaa aggcacttgc gcttccagac cgtgcgccac tttactttgg
    16741 tgcgctttca ccgcgtaatc aatcacatcg cgagtttgtt ctaacagatc gagcatctga
    16801 tagctggcct gcatactgta ataaagctgc gctacgcccg tggtcagcga tagctctact
    16861 gctgcggttt ctgccagcgc ggcattatgc gcgccaatgg cggcggtaac cgctgagcga
    16921 tgcacacccc acaaatcaag atccagtccg gcaaacaaac cgactgtggc ttccgtatag
    16981 tacggcccgt ccatacccag tgctggcgca tccatcgcat aagggcttaa aaagccgttc
    17041 gccgagacgc gttggcggtt gagcatccct aacgctgcga cctgtaattg tgaaccggca
    17101 tctaacaaat cggcctggga ctgcgctttt tcttcccgca gtttcgcttc ggcgagggtg
    17161 tgtgaaccac ttagcgtccg ttggatcagc gcatccagct gcgggtcatt gagttgtttc
    17221 caccactgcg cctgcggcca gccggagctg gcaagatgaa tatcatcggc cagtttgatt
    17281 tgttccggtt tgagctgttg atgaggcgca gaatccttgc gtaccagggc acagccggaa
    17341 atcagcgtcg tgctgccgag aatgctgcac aacagcagac gtgaaagttg acgattgatc
    17401 attgcgtggc tccttgtgcc tgttccgtgg cgggcgttaa tgccggggct gtccagtctg
    17461 gcaagcgggc gaaaagctgg cagacgtgtt gctcctgttt taataaggtg ggcaggatgg
    17521 cctgcgaggc gggtgtctct tcaagcgtta tttgaggaga acggctgagt gcggttgcca
    17581 gaccggcagc gtatttttcc agggcgtcgg caaactgacc tgctttgtcc ggctgtagtg
    17641 cgttatccag cgcctgcgcc gagttccagg tggcatccca ggcgtgaaga agatcgcggc
    17701 cctgatgaat aaccgtttgc gaacgttcaa tcagtaaggc gcgttcttcg ctgtccagtt
    17761 gacgctccag cgccacgcgt tggcacatct cttcacaggc attaaacgcc gcatgcagac
    17821 ctatacgaat ttgcagataa gtgcgcagag ccgtgacttc ctgctggcgt gggatccgca
    17881 ttactttact tagcataccc agcgcgccag ccagtttttg cggcagcgtg cgcgcttcac
    17941 tttcaggcca gacaaaggtg taaatcaccg cagacaccac ggtaccaatg atgataccca
    18001 gggcgcgatc gcgaatttcc accaggtcgt acaccggacc aaaaacgttt tcgagcgtgg
    18061 cgagcgcgaa ggtgaccacc atctgtgtgc cgatataaga agagcgttca gagctggtgg
    18121 caatccatgc gcccaacagg aaaatcggtg ccagcacaaa cagcaattcg acaatattgt
    18181 ccagccaggg catgaccagt agcgtgaata acagcgccag aatcgcgccg caaaaggccc
    18241 cgccaaaacg cagcaccatc ttctggtacg atgaaccgac attcggatta gcgacgatca
    18301 cgcaggtcag catacaggtg tgaatgcctt cccagtccac gccgctgtag aaggtgtaac
    18361 agatcaaaca ggcgagcagc gtttttaccg cgtagcgcat atagtctgga ttggtaaaag
    18421 catcagcgac cattgatggc ggtttggcgg cgggcgttgg cggcgtattc gggtccatct
    18481 gacccagttg taacaacgtc tggcagatat tctccaggtt acattcccgt gccgccatcg
    18541 cttcactttc actgatccgc cagtcgcttt gccagcactg accttcggta atggcatgct
    18601 gcagcttgtt gatttctgaa gctaattttt gtcggaattc aataattgcc tgagaatcag
    18661 caaaagaggt gggatcgtag cgattcagcg tcgagtaaat gtaggttacc gttgccacgc
    18721 agctttgcca ccatgcgctt tgagttcgcc agttggcatc gtccgcgagg caaaagacat
    18781 tgagtttttg cagcgccagc gcctctcttt caatccgcgt ttcgggtagc ggtgcgaggc
    18841 tgtccgtcag gtggctaatg gcatcatcaa gccgatcatt aagcgcttga tgcatttgcg
    18901 aaatggcacg actgggaaac cacagtacgc cgattaacgt cattaataac gtcgggtaga
    18961 ggccaacaac gatacaccac agcgttaagc gcacgaccac ttccggatag tcgagcatgg
    19021 cggggaaggt ttgcccataa atggcgacaa tggcgacagc gaaaaagacc agtcccaacc
    19081 gatgggtgcg catcaaaaac atgcagccca tcaggatcgg cccggcgatg atcaagcgga
    19141 tcaacggttc gccgtatgac catttgtaga tcaaaaacag gctgccgatc tccagcaccg
    19201 tggcaaccac aaacaagatc gcgacaaatt tggtgtaaaa cgcgttcgac tgaataccgt
    19261 aaaacagcac tgccagcgat aacgccacaa aagggatctc aaaagtcatc gagatcagaa
    19321 tcaccagcag gcagcctacc cagagttgca cggtctgcgg cacgcgacct ggtcgccgct
    19381 cgcttaactc ttcatgaaag aacgccagca gcctgaccac cggtaatggc agggagttga
    19441 gcgcgctcat tattgcggct caagattagc gactgccgaa gcgccgatgc ggaacatttc
    19501 cgggtcaggt ttatcaacca tgattttgac cggaaaacgc tgggcaacac ggacccagtt
    19561 aatagaacga gaaactttcg gcaggccgcc cagcaccagg ccgccgtcat ccggtagcac
    19621 gccgtagcca attgaatcca ctttaccctc gaaagttttg ccgctgtcac tcatcaggcg
    19681 aatcgttgca ggtgtacctg agcgaatatt tttcagatcg gtttcgcgga agttggcgat
    19741 cacgtaccag tgacgagtgt cgattagggt aaaaataggg cgcatggcag aagcaaattg
    19801 cccgacggag gttttgaggg aaatgacccg gccatcaaac ggtgcgcgaa cggtcgccat
    19861 ttccagatgc agtttggtca gggcaatatc cgcttcgacc gccgcacgct gggcaactaa
    19921 tgcatccacg ccgctgacgg cgctggcggc tgactgcgcc tgcaacaata cagcattaag
    19981 atcggcttct gcagcgcgct gcgcggtcct cgcacggtca acgtcttccg ctgagacaaa
    20041 accttctttc agtaatggct cggtgcggcg taatgtatct gtggcctgtt tagcggcggc
    20101 acgggctttt tctaccgtgg cgttaaccga gtcggcacca aactgttgcg cgtcaacgct
    20161 acgctgggtg agcataattt gcttatccag tgccgcgagg gaggcttcag atttcgccag
    20221 attggcttcg tacgggcgcg ggtcaatgcg gaacagcaaa tcgccctgtt tgactgcctg
    20281 gttgtcggtg accgccagtt ctacaatgcg gccgctgact tccggcacca catcaatggt
    20341 atctgctgac acgtaagcgt cattagttga tggcgcactg tctacgcgcc agataacgaa
    20401 aacaagggca accagcgcca acgcaaccac taacagagca gggaatttac tgcgaggagc
    20461 ttttttcggc gtactttcca tgagcgcgtc tcaaatagat tagaaaaatg ccagccagaa
    20521 cagcatggcg tagagagcaa aaagggcggt ataaataatt ccgacaaatg ccagcttgat
    20581 atttgcccgc tgaataactc gcctcgtgat gagcgtcaat ataaggctgg caatggcgca
    20641 gaaaaaccag ctgggataat aagcgccgat caccgggatt gccggagata atgagcatcc
    20701 cgaaaccatg aagacgggaa ttatccaggc tgtttttttg agttgcatcg ccatgcgaga
    20761 gagaactgtt ggcatattaa atacatcctg tctgctgcga acaatatctg tagtgaatat
    20821 caatatccat tgaatgcgtc agtatggcta ttttcttgat gaataaaaat agctttattc
    20881 agccaccgca ttcataactt atatatttta tttattaatt ggctgtatat atttttaaaa
    20941 taaatgcttt tgggggcttc agcgccccca aaagcgagtg acattaattt ggggttacga
    21001 tattcaccca caaatcgaaa ttatccagac aggcgataat ttcttccagt ttcgcgccat
    21061 tgccaataat tttggctttc ttcgctttca ccagatccgc catttttgcc tgtccggtca
    21121 gcacggcgtg cagatcttca cggctgatgt agaaagaggc gtcggcttgc ggttgcagtg
    21181 ttttacggta gttaagcacg ctatcgttca gcgtcaggtt gaggttatcg ccgttgctca
    21241 tattgaagtt caggctgata tttttacccg cagctttcgc gctatcgagg cgaacggcca
    21301 taaagtcgaa cagcatttcg accgacattc cgcgaatggt gtccggggaa ccggtggtgc
    21361 cgtggctgaa cttatgcacc ccttcgcgca gctctttcgc gccggtcagg tagaaaccgc
    21421 gccatgtggc ggactcggcc tgatagccca gctgttcaaa gttattcgct tgcagattct
    21481 tcgccacctg gtcacccggg ttggcggcaa tcacctgttt cagtagttct gccgaccagc
    21541 ggtaatcacc ttgcttattc gcttcttgcg ccaggttgat gacacgggca gagccgccca
    21601 gcgctttcac gtaacgtttg cccatctcta cctgaccata cggatgcagg ttagccgggt
    21661 taccgtcgta atagccaaga tagaagttat acaccgcgcg ggcgttgtgg ctgacagaac
    21721 cgtaatagcc acggctggcc cagttattgg caagtgcagg cggcagctta atcatgtcgc
    21781 cgatttcatt catggtgtag ccctggttcg ccaggtgcag ggtctggtcg tgaatgaacg
    21841 tgatagtatc gcgatattta ccaatataat cattgatatg cttattgccc cagaccggcc
    21901 atgtgtgcgg cataaacagt acttccgcgt cgttacccca catatccagc gtttcgttca
    21961 ggtactcggt ccacttgctg gtatcgcggg ttttcgcgcc gcgcagagtg tagaagttgt
    22021 gcagggtatg cgtggcgttc tcggcggtac acagggcttt cagggccgga atatagaagt
    22081 gcatttcggc tggcgcttcg ctacctgggg tcatcaggaa gtcaaactcc aggccgtcga
    22141 taatcatctt ctcgccagtt ctgacgatag ttttagtcgg tgcaataatg ctcgggtcgc
    22201 ccgttgccag cgtcacgcca aggccattac ccacgttgcc ttgcgcgttg tgcggcagta
    22261 acagaccgta agagtagagc gcacggcggc tcatgatgtt acccgccagc acgttttcgc
    22321 tgatggcttc gtccataaag ccagcagggg caatcacctg aactttgccg gatttaacat
    22381 cggcttcaga gataatgcct ttcacgccac catagtggtc ggtgtggctg tgagtgtaga
    22441 taacggcaac aatcggtttt tgcggacgat gctggaagta aaggtcaagt gcggcttttg
    22501 cggcaggcgg cgtcaccagc gggtcgatga caataatgcc tttctccccc tcaacgaacg
    22561 taatgttaga gatatcctgg ccgcgcacct gatacatttt atcggtgact ttgaacaggc
    22621 cagaaatgcc gttgatctgc gactgacgcc acaggctggg gtttacggtt tccggcgcag
    22681 cggcattaat atcaaatttg taatcgtctg cgcggtagta aactttaccg ttcgcatcac
    22741 gcagaatacc ttcatccagc agcggggcga taaatccacg ctgggcatcg tcaaaatcct
    22801 gacgatcgct aaacggtaat gatttggcgt aattctgatt aacctgttgg gtatattgag
    22861 tggcggcttt cgcttcctct ttcgcgttag cggtatttac catcatgccg ctggcggcag
    22921 ttaacgcaat aacaatcctg ctcaaacgga ataaccgaga gttattcata taaatctcca
    22981 tttatcattt atgtcgtaaa tatgtaatcc atgcaaaata cgtcatttaa agctggcgtt
    23041 atcataattg ggaaattgcg actttaataa gtggaagtgt gagcggaacg atccatttta
    23101 ttaggcattt gtgctgaaga gggaaaagta cgacatattt attactatca tcaataatgt
    23161 gacctgactt atatttttat tatttatatt actttcagct ttcacttcga caagaagtac
    23221 gcacaatgat taatcagatg aatgatgcgg agtgtaacca tgaaacccgt cgtgctggtg
    23281 gtggatgatg acacggctat ctgcgcactg ttgcaggacg tgttaagtga acatgtcttt
    23341 acggtttcag tctgccatac cggacaggag gccatattgc gtattgaggg cgatcctgac
    23401 attgcgctgg tggtgctgga tatgatgcta cccgatacca acggcctgag ggtattgcag
    23461 caaatccaga aactgcgtcc gacattgccg gtggtgatgt tgaccggcat gggcagtgag
    23521 tctgatgtgg tggtcgggct ggaaatgggc gcggatgatt acatctgcaa accgttcacc
    23581 ccgcgggtgg tggtcgcacg actgaaagcg gtgttgcgac gggttggcgc gctggcggtt
    23641 aatgacgaga aaagcgccgg gctgtcattc aatggctggt atctcgatac catgcgctgc
    23701 cagttgcaca atccgcttca gcaacatgtc gagttaactc agggcgaata tggcttgtta
    23761 ctggctctgg cgcaaaacgc gcgtcgggta ttgagccgcg agcagttgct ggcgttcacc
    23821 cacagcgaca gcgtggaggt gtttgatcgc accatcgacg tattaatcat gcgcttacga
    23881 cgcaaaatcg aactcaaccc gcatcagccg atgttgatta aaactctgcg cggtctgggg
    23941 tatgtcttcg ctgccgacgt gcattaatcc gacatttctg tttgcagctc ttccagcgtt
    24001 ttcgccccgt cgatggcatt ggcgcataac aggcttgccc gcgcgcaggc gtgtgccagt
    24061 ttgatgctct cggtaagcgt ccagcgttca tggcagccat ataaaaatcc ggcacaaaat
    24121 gcatcgcccg cgccgacgct gccgataatt tcccgttgtt ctaacttttg cgaagggatc
    24181 cacacgccgc cctgttctgg ggtctctccc cacgcccctt ccgggtagtg gatcaccacc
    24241 cgctggcgca cgcccgcatc taacagttca cgcgcggcgg cagcaatatg cgcgatatgc
    24301 ggttcgccgt tgggtaaccg aatttccagc ccgctaaact ccccggcctc cagttcgtta
    24361 attaccagat aatccagatg gcgcaacgcg ggcagcacca gcggctgata acgcggatcg
    24421 cccttacggg agaccaggtc gagtgaggtt tcaaagccag tatcacgcat ttgcgccagc
    24481 aggcgtgcgc tacgggtgcc gtaaacatcg tccggcatat ccaggctgtc gagcaacagt
    24541 aaatagccga gatggaaaat cttcatggtg ttatcgagct gatcgaacgc cggaagatcc
    24601 aaaagtctgt ttgcacccgg cgaatggaaa aacgtccgct gcccgcttgg gtctgtcatc
    24661 acctgcgaca tcgacgtcgg ggcaaaggta gtgcgctgca catgctgacg attgacgtga
    24721 tattgctcaa gcatcgccat gatgtaatcg ccgtcgctgt cctgaccgat tagcccaacc
    24781 gcctgtaacg gcagcccggt gcgcattttc gccagcgtca gcaggacgtt aagcggcgca
    24841 ccgccggtgg agcgttcgct gtgggtgatc tccgccagcc agccgcgctc tggccactgg
    24901 ataatctgat ggacgtggtc aaccaacata ttgcctgcgg cgataatccc tttacgctcc
    24961 atcatgcctg ccccgcactg ccaaaaatac gcatctgttg tgcaaccgta tcgctgatgg
    25021 cggcttccac tgccagcagt aactcggcaa actcgtcata catcggctga cggtgcgcca
    25081 ttcgggcctc aacggcagcc agcgccgctt gcgacatgcc agtgtagaaa ttgattttgt
    25141 ggatacccag ttcgatggcg cggcggaaat cggcatcgct aatccctgag ccgccgtgta
    25201 ataccagcgg gatagcagcc tgctggcgga tagcgtcgag gcgcgggaaa tcgagtttcg
    25261 gttcgccgcg atatttgccg tgtgcattgc caatcgccac ggccagcgcg tcaatgccgg
    25321 tacggtcgac aaaatcacgc gccagttgcg gatcggtaaa cagggattca tcggcatgac
    25381 catagagtgc accgccttcg tcgccaccca ccgcgcccag ttcagcttct accgatacgc
    25441 caacggcatg gcacattttc accacttccc gcgtctggcg gacattttct tcatacccca
    25501 gcgtggagcc atcaaacatt accgagctga atcccagccg caacgcttgc accaccgatt
    25561 caaaatgcag tccgtgatcg agatttaaca ccaccggaat gtcatgacgc gccgcttcaa
    25621 atttcactgt ctcgaccagg gaatccagcg agatatattt aaaatgtact tcggcaatgt
    25681 tgataataaa gggcgaacgt tcttgtctgg cagcagcaaa caaggcgcgt aaaaagtgcg
    25741 agtccagcac attaaacgca cctaacgaat agtgatgttc gcgggcatgt gccagccctt
    25801 cagcgagaga aatcaatggc ataacgttat ccttattcag cggaactggc ggtattcgtt
    25861 acacaatacc aactgtgcag gttcgtcttc gtcaatcagg ttgtagcggt ccagtggctg
    25921 taaaaagtgg ttatcgtggt cgtcgtcatt aacagaagag acttcgccaa ccagcacatc
    25981 gccaaaaccg gcttctgccc aaaagctgtg atacaggccg ggcggcaggc agatactttc
    26041 gccgggagac aggcgcaact gagagcctgc agtatgtttc tggcggcagc cgtctatcac
    26101 caccgtaatg tcactatcag ccgtttgctc gttactatcg gcattccaca gttcaacaat
    26161 taaattaccg ccgccgcgat tgataatgtc ctcacgctta cgccagtgaa aatgcattgg
    26221 cgtgacctgc gcatcgcgca catgcatgat cttctcggcg taacatttca catacggcat
    26281 tccttttgct gagccgttac gtagggtaaa cagcgtcagc ccgtgtgctg caaagttgtt
    26341 gcgaccaaaa gcggtaacat cccagccgag tttaagatca aaaacctcct cccacgcggc
    26401 ggtgtcgagc tgctgccatt gagcaggcga gaaactggca aagggcggca gatgtacgtc
    26461 atgttgcgaa aagaattgcc gtgtatggcc gagaatgtcg tttatagcgg agcgtttcat
    26521 cgaaagcgtt ctccttagcg gtgaccgggc agctgcgccg cccggcgtgt ggacttattt
    26581 caccgtccag cctttgtaat tgccgacgtt gtttttatcg atcatcgtca ccgggatcag
    26641 caccggctct ttcggcgcag gtttgccctg caaaatgtcg tagccaattt ccaccgcttt
    26701 cgctgccatc acctggggat cttgcgccgg agtagcgaca aacaacgagt tctcacgctt
    26761 gagcgcctct tcggcgtccg ggcttccgtc cacaccgaca ataaagaact cgttacgttg
    26821 tgcctgtttt gctgccagat cggcaccgat tgcggtggga tcgttaatcg caaatacgcc
    26881 gtcgatcttc ggatttgccg ccagcagtcc cgtcattacc tccagcccgc cttcacgact
    26941 gcctttcgcg ttctggttgt cagagagcac tttgatgtca ggatggcgtt taaattcagt
    27001 ctggcatcct tcgacgcggt tttgcacggc ggataccggc gggccgttga tgatcaccac
    27061 gttgcctttg cctttcaggc gatcggtgat gtatttacag gccatttcgc ctgcctgggt
    27121 gttatcggaa gtgatggtgg catcggcacc ttctgccgct acgtcaaccg cgacgaccac
    27181 gatcccggca tctttcgccc gtttcaccgc cgggccgatc ccttttgaat cggcagcgtt
    27241 gaggataatc atatcgacgt tagcggcgat aaagttgtct atctgcgaca cctgctgacc
    27301 cagatcgtag ccgctggaaa ccagcgtcac tttgacgtta tcgcctgcca gtttgcgtgc
    27361 ttccagctcc gcgcctttgg tgatctgcac gaagaacggg ttcgccagat cgcccaccgt
    27421 tacgcctatt gatttcagtt ctttcgcctg ggcgaacggt gctgcggcca gcagcgcgcc
    27481 tgcacagagc gcggtcacta agggcttcaa tctcatgctg tattcctcat gtgtttatgc
    27541 actttgatgg tgtcgggtac ggtatttgtc gatcagcacc gcaatgatga tcatcgcccc
    27601 tttgatcacc agttgccaga agtaagagac gcccattaag gtcatgccat tgttgagcgt
    27661 ggcgataatt agcgcgccga ccagcgtgcc ggtgatggtg ccgatgccgc cgacaaaact
    27721 ggtgccgccg aggatcaccg ctgcaatggc atccagttcg taacccatgc ctaagttgcc
    27781 gttggcgctg tacagccgtg aggcgctcat aatcccacca agcccagaaa gcaggccgct
    27841 catgccataa acaaacagca gcaccagcca gaccttaatg ccggtaagac gcgccgcctg
    27901 catattgccg ccgacggcgt agatatgaac gccaagcgtg gtgcggcgca gaataaacca
    27961 gcacaccacg atcaccgcca gcgcaatcac caccagccat gggaccggcc cgagatagtt
    28021 gttgcctatc cactcgaaat taatgttgga gttgataacc gtggtgccgt ccgccagcag
    28081 atacgccgcc ccgcgcagcg ccgtataggt gccaagcgta acgataaacg gtggcaatcc
    28141 ggcaaaggcc accagcgcgc cgttgaacag ccccagcagc aggccgagca tcagcgctgc
    28201 cgggatcgac agcatggcga actcagggct aagggagacg accatcgccg ccaccgctgt
    28261 ggtgccgagg atcgaaccca cggaaagatc gatcccgccg gtcaaaatga tgaaggtcat
    28321 ccccgccgcc agaacaatgt tgatcgatgc ctggcgggta atattcagca ggttgctttc
    28381 ggtaaaaaag tttggcgtga taaagccaaa taccgccacg atcagaatca aaatgggcaa
    28441 gatgccgacc gtttgcatca gatcgctcat cagcattttt ttggcagagg tttttgcaga
    28501 ggtcgtcatg aggttaccgc ctgggaatgg gcgtcgttga cgccagtcgc taaggtcata
    28561 atgttttcct gggtgatgtt tttgccgttt aactcaccgg caatgctgcc ttcgcgcatc
    28621 acatagacgc gatcgctcat tcccactatc tccggcagtt cgctggagat catcaggatc
    28681 gccacgccct tgcgcgccat ctcgttcatg atccggtaga tctcactttt ggcgccaacg
    28741 tccacgccgc gggcgggttc atcgagcagt aaaatgcgcg ggccaatcgc cacccagcgg
    28801 gagatcaaca gtttttgctg attgccgccg gaaagcccgc ccgcgcgtac ctgggcatga
    28861 ggcacgcgaa tgttgagcaa cttaatggcg tcatcggaaa tggtttgcgc ttttttgcga
    28921 ttgagcattc cccagcgggc atcgcgctcc agggttgcca tggtgatgtt ttcggctgct
    28981 gccatttcaa ggaataagcc ttgttctttg cggttttcgg tgagaaaacc aatgccaaga
    29041 tcgatggctt cgcgcgggga gtggatcacc accggttcac catcgacttc aatcattccg
    29101 cctgtcgctt tccgcacgcc gaagatcaac tgcgccagtt cggaacgtcc ggcacccacc
    29161 agtccggcga ggccaacaat ttcgccggaa cgcaccagca ggctactcgg ctggactttg
    29221 ccgccgtcgg tcaggtggtg aacattcagg cgggctttac cgagcgggat atcgcgctct
    29281 ttattgaaca gatcgctcag tggcctgccg accatcatcc gcaccagctc cccggcattg
    29341 aggttatcgc gggtcaggct gccaacgtat tgcccgtcgc gtagcacgct gacgcgatcg
    29401 gaaagctcat acacttccgc catgcggtgg ctaatgtaga taatcgccat cccttcatcg
    29461 cgcagccgca taatcagttc aaacaggcgg tgagtttcgc gggaggagag ggcagcggtg
    29521 ggttcgtcca tcaccagaat gcggctgttg cgatgtagtg cgcgggcgat ttcgacctgc
    29581 tgttgctcgg caatggtcag cgtcatgacc ttatcgctgg cgttaaactg cgcgccgagg
    29641 cggtcgatca ctttttgcgc ctgcactagc atctctttac gctgcaccag cccgccgtgg
    29701 gaaagctcgc tgccgagaaa aatattttct gccaccgaca gattcggtgc cagctgcatc
    29761 tcctgataaa tcagcgtaat accggcgctt aaggcatctt ttggcgtgcg aatgtgaaag
    29821 ggcttgccgt ctatgagaat ctcgccgctg gtggcggtat acgcccccgc cagcaccttc
    29881 atcagcgtgc tttttcccgc gccgttttcc cccatcaggg catggatctc gccagggtag
    29941 accgtcaaat ccaccccttt gagtgcataa aatttgccga aggctttggc aatattgcgc
    30001 atctccagaa ctggcgtgct gttcatggtg gactcctgcg aggttgtcga tatcagcact
    30061 ccatcacagg aaagtaaatg caaaatgtca gaagccgttc atatttgtca tattgcgatt
    30121 tttcttcacg gagcgtgata tggcgcaccc cggacgtcac ttttttgcca gcgcgcgcgg
    30181 gcgattgctg cttttgaatt tgctggtggt ggcggtgacg ttgatggtca gcggcgtggc
    30241 ggtaatgggc tttcgtcatg ccagccagat gcaggagctg gtgcagcagc aaacggtgga
    30301 tgatatgacc ggcagcctga atctggcgcg cgacacggca aacgtggcaa cggcggcggt
    30361 gcgattgtcg caggtggtgg gagcgctgga atataaaggc gaagccgagc ggttgcagga
    30421 gacacagcgg gcattaaaaa gctcgcttgc acaactggcg aacgcgcctt tagcgcagca
    30481 ggaagccggg ctggtgacgc gaatcatcac gcgtagcaac gaactgcaaa ccagcgtcgg
    30541 cggtatgctg gagcgcgggc aaaggcggca tctggagcgc aacgcgctgt taagttcgct
    30601 gtatcaaaac ttaagttatc tgcggcatct gcaaaaagtc actcacgcgc aagatgatat
    30661 tttgcttaac gagatgaacc gactgattgt tgccgctatt gccacccccg cgccgcaggc
    30721 gattattcat caactggtcg gggtaatgtc tgcgttgccg acccacagcg atacgccatt
    30781 ggttaatacg ttactcaatg atttcaatga tttcaatgat gaaatgcgta aactcgcgcc
    30841 gctttctgcc gctctggagc aaagcgatct ggcaatcagc tggtatatgt ttcacatcaa
    30901 agcgctggtg gcgatcctca atggcgacat caaccgttac gccgaacagg tggcgacgct
    30961 ctccggtcag cgtgtggcgc aaagttacca ggatttgcgc tccggcgaga gggtgatcat
    31021 gattttcgcc ctgttagcgg tggtgatcac cgcgtttgcc ggctggtata tctatcgcaa
    31081 cctcggcact aacctgacgg cgatttcccg cgccatgagc cgcctggcgc acggcgaagc
    31141 ggacgtcact tttccggcgt tacagcggcg tgatgagttg ggtgaactgg cgcgcgcctt
    31201 taacgttttt gcccgcaata ccgcgtcact cgaacgaacc tcacggctgc taaaagagaa
    31261 gaccacgcag atggagattg cccaaactga gcggcagggg ttggaagagg cgctattaca
    31321 cagccagaaa ctgaaagctg tcgggcaact gacgggcggg ctggcccatg attttaataa
    31381 tctgctggcg gtgattattg gcagcctgga gctggtgaat ccggactcgc cagacgcgcc
    31441 gcgaatacag cgggcgctgc aagctgccga acgtggggcg ctactgacac aacgactgct
    31501 ggcgttttcc cgcaagcagt ccttacatcc gcaagccgtt gaactgaaaa cattgctgga
    31561 agagttaagc gaattaatgc gccactcctt accggcaacg ctggcgctgg agattgaagc
    31621 gcagtctccg gcgtggcccg cgtggattga catcagccaa ctggaaaatg ccatcatcaa
    31681 cctggtgatg aacgcccgtg acgcgatgga agggcagacg ggaatcatca aaattcgtac
    31741 ctggaatcag cgcgttactc gcagcagtgg gcagcgtcag gacatggtgg cactggaggt
    31801 catcgatcat ggctgcggta tgtcgcaggc ggtgaaaagc caggtatttg agccattctt
    31861 taccaccaaa cagacgggca gcggcagcgg gctgggtttg tcgatggtct atggttttgt
    31921 gcgccagtcc ggtgggcgcg ttgaaattga aagcgcgccg gggcagggga caacggtcag
    31981 attacagctt ccccgcgcgc tgacggcggc gaaaaaccta tcgccagcgg cagtagaaca
    32041 ggcttctgtc agcggcgata aactggtgtt agtgctggaa gatgaagccg cggtgcgcca
    32101 gaccatctgc gaacagttgc acctgctggg ctatttgacg ctggaagcaa gcagcggcga
    32161 acaggcgctg gatctgctgg cggcatcagc agaaatcgat atttttatca gcgatttaat
    32221 gctccccggc ggcatgagcg gcgcagaagt ggtcaatgcg gcccgcaaac tttacccgca
    32281 cctgacgctg ctactcatca gcggtcagga tttacgcccc agccataacc ccgcgctgcc
    32341 agacgtggcg ctgttgcgca aaccctttac ccgtgcgcag ttggcgcagg cgctggggca
    32401 agagaattga gaatcctcct ctactcgtaa caggcacctt tgcctacctt gtattaactt
    32461 tgttgcgagg ccgagtttat gaaacgccac tccacgcttt ttcttttcac cctgctcacg
    32521 ctgaccaccg taccggccca ggccgatatt atcgatgaca ccattggcaa tattcagcag
    32581 gcgattaacg acgcttctaa ccctgaccgt gggcgagatt atgaagattc gcgcgacgac
    32641 ggctggcagc gtgaagtgag cgacgatcgg cgcagacaat atgacgaccg ccgtcgccag
    32701 tttgaagatc gtcgcaggca gctggacgat cgccagcacc agcttaatca ggagcgtcgg
    32761 cagctagaag atgaagagcg gagaatggaa gatgagtatg ggcaatgatg ttgggtatgg
    32821 ggcaggcatc gcccctctgg ggagagggtt agggtgaggg ggccagcacg cacattccct
    32881 ctcatcacgc caccccaatc tccatcccat caaaccccgc ctcaaacccc gacggcagtg
    32941 cgttttccat cagccacgca tcaaactggt ggctgatatg ggtcagaacc acgcgcggcg
    33001 agcggataac ctgattcagc gcaagcacgg tatttaaatc acagtgatta cgcggtgcat
    33061 cctcgcgcgg cgggtggctg caatcaatca ccattacctg cggctgatta ttgcgtaaaa
    33121 atttcagcgt tttttccggc aagccagcgg tgtcagacag ccacgccacc cggctgtgcg
    33181 ccgtttccag cagataaccg aaggtcagtt ttgagtggtt gagcggcagg ggtgtgacct
    33241 gtaacccctg caaatcaaac accacaaacg gttccaccgt gtggctgaaa tcaagcaggc
    33301 ccggatgttt aaacagatcg tcgcagccct gttcatccgg tgggccgtaa accgggatcg
    33361 gatcgcccac gccccagcgc agcgggaata gcccctgcac gtgatccata tggtagtgcg
    33421 tcagcaaaaa ctgctggaac gatccgggcg accagcgatc ggcgagatcg tgccgcccgg
    33481 cgtcgatcag ggtgatcgcc tcgttaaact tcactacgcc gctgcatggc tggcggcgat
    33541 agtgcggcga gcgccgcgct ctggcgcagg ccgcacactc gcagccccat gccggaacgc
    33601 cctgtgcgcc gccggagccg gtgagcgtga gggtcaggct catgttacag caccttggtg
    33661 aagcggaagt ggctctgctc gtagccttcg cgcagataga aacggtgcgc gtcgtggcgc
    33721 ttcacgttgg tcgaaagttc ggtcatttcg gccccggcct ggcgggcttc ttcttctgcc
    33781 cacgccagta acttactgcc gacgttcaga ccgcgcgcct gcggcattac caccaactcc
    33841 tgaatttcgc cgatccagtt gacatgatgc agatgaaact gcaaatgcag gccgatcata
    33901 ccgacaactt cgccatccag cagcgccaga tggtagcgca tgttcgggtc gcgcaggttg
    33961 gcgttaaagc ccacgcgaaa cgcgtggtgg tcaaactccg cctgttttag ctcgcaaatc
    34021 aacgcgtaaa ccgcgtcggt gtcgtactgc gtggccgggc gaaagctcac aagcaggcat
    34081 ggtgtttctc cttatgatgg attagcgtca gcagcgtgtc gaccgactgg cgcaggctgc
    34141 cgtcattatt gagcgtatgg caatcctgtg gagtatagcg ggcggcgcgc gccagccggg
    34201 cgttaatttc actggcgttt tcgcggccac ggttttccag gcgctggcgc aggatctctg
    34261 gcgaaacctg taagcaaatg ggcagcagcg ccgattgata gcgcgcccgc gcctgcggca
    34321 gatgggcgcg tgagccgttg accagcacgt cgaatccagc gtgcagccag aggtcaatct
    34381 cgacgccgac gccataatac agaccgttag cgtgccagct taaggccagc agattttgcc
    34441 ccgcgcgggt aaaaaattcc tgctcgctca gggcgatatg gttttcgctt ccggcgctgg
    34501 cgtcgcgcgt gatgtagcga tgcgccacca gtagctgagt ttgttcccgc aggcggagtt
    34561 ccgccagcag gctatctttc ccggagccgg acggccccat taaccaaatc agttttccca
    34621 tcatcagaac acccttttac cctgacgcca gacgtggtcg atatgaatgt gattgccctt
    34681 gcgatgcgcc agcaccaggt cggcgcgttt gccctcgcca atcaccccgc gatcctggag
    34741 attaagcgcc tgcgccggat ttttggtcac cagcttcacc gcctgcggca gcgtaaagcg
    34801 gttgctctgg tcatcggcga cgcggaacgc cgcatccagc aggctggcgg ggtagtagtc
    34861 ggaagagagg atatccagca ggccaagctg tgccagttca ctggccgcca cgttgccgga
    34921 gtgcgaaccg ccacgcacga tatttggcgc gcccatcagc acgttcatgc catgcttgcg
    34981 cgaggcttcc gctgcttcaa acgtggtggg aaattcggcg atcacgctgc caagctggtg
    35041 agattcggca acgtgggcgt gcgttgcgtc atcgtggctg gcgagcgcaa tttgtcgcgc
    35101 gcggcacatt gcggcgatgg attcgcgatt cggctgcgac cagcgtgcgg caagcgccag
    35161 ctgttcctct tcatactgct gcatctgcgc atcggtgagc gagtatttgc cctgataata
    35221 ttcgcgatac ttctcgcggt tggcgaactg gcgctggccc ggcgagtgat ccatcagcga
    35281 caccagcgtc accggctcgc gctgcaccag cttttcaaac agcggcagcg tggtgttatg
    35341 cggcagctcg cagcgcaggt gcaggcggtg ctcggcgcgg ttgacgccgc gtttctgcgt
    35401 ctcttcgatg gcgttgatca tcttctccag attctccagc cgatcgccgc cgtctcgcac
    35461 gtcgccaatc gccacggcgt ccagtacggt ggtgatgccg ctcgccacca tcagcgcgtc
    35521 atggctgctc atcgccgagt gggcgggcca gtcaactttc gggcgcgggg tgaagaattt
    35581 atcgaggttg tctgtatgca gctcaatcag ccccggcagc agccagccgc cttcgccgtc
    35641 catcgcctcc ggcaggcggc tctggctttc ggcaaaggcg cggatttcgc cgtcctgcat
    35701 ctccagcgaa ccgtttacca cttcgttttc cagcaccagt ttgacgttat tgataatcat
    35761 gaagaggctc ccattgggtg caggcggtcg gcgacgtcat tacgtacggc ttcgtcatgg
    35821 aaaatgccga cgatggctgc gccgcgggct ttggcttctc ggatcagttc gactacggcg
    35881 gcgctgtttt tggcgtccag cgaggcggta ggttcgtcga gcagcagaat ggggtagtcg
    35941 acgataaagc cacgggcgat gttgacgcgc tgctgttcgc cgccggagaa ggtcgatggc
    36001 gcaaggcgcc acaggcgttc cggcacgttc agacgggtga gcagacgcgc ggctttagcg
    36061 gcgcaggctt cacgcggtac gccggtatcg agcagcggct gcattactac ttccagcgcc
    36121 gaaatgcgcg ggataacgcg cagaaactgg ctcacccagc caacggtggt tttgcggatt
    36181 tccaccactt tgcgcgctgg cgcagtgacc aggtctaccc actcgtcacc gtgtttgatc
    36241 tggatttgcc cttcatcggg cagatagttg gcgtacagcg agcgcagcag ggttgatttg
    36301 ccgctgccgg agtggccgtg gagcaccacg cattcgcccg cgttgacggt gagcgaggcg
    36361 cggttgagga cgggcaagcg cacgccgttt tgctggtgca ggatgaaggt tttactgacg
    36421 ttttgtacgt taatcatttt tcgcctcgtg gttcgggtgt cggatgcgac gctgatgcgt
    36481 cttatccgac ctacggggag cgcatttgta ggccggataa ggcgtttacg ccgcatccgg
    36541 caccggctca attctgcaaa accgatgaca ccagcagctg tgtatacgga tgatgcgggt
    36601 cgtcgagcac gcggtcggtt aacccactct ccaccacttg cccctgcttc atcaccagca
    36661 aacggtccgc cagcaggcgg gcgacgccta aatcgtgggt gacaatcact accgcgaggt
    36721 tcagctccac caccaggccg cgcagcaggt cgagcaggcg ggcctgcacc gacacatcca
    36781 gcccgccggt cggttcatcc ataaacacca gcttcggatg cgtcaccagg ttgcgggcaa
    36841 tctgcaaacg ctgctgcata ccgccggaaa aggtggtcgg caggtcgtcg atccggttgg
    36901 cgggaatttc cacctcttcc agccacttct gcgcggtggc gcgaatatcg ccgtaatgac
    36961 gcgccccggt cgccatcagc cgctcgccga tattgccgcc tgccgagacc tggcggcgca
    37021 ggccgtcgag cggatgctga tgcactacgc cccattcggt gcgcagcagg cgacggcggt
    37081 cggcctcgct catgccatag agggaacggt tttcatagtg aatctccccc tgctgcggcg
    37141 tcagacgcgc ggaaattgat ttcagcagcg tggtcttccc ggagccggat tccccgacaa
    37201 tgcccagcac ttcccccggc cataaatcaa aagagacatc gctaaagcct ttgcccggcg
    37261 cgtaaaggtg ggtcaggtta ttgaccgaaa gtaacggttg attcattggc tttttgcctc
    37321 gctctgttgg cggcaataat cggtatcgga gcagacaaac atgcggtttc cggcgtcatc
    37381 cagcaccact tcatcaagat agctgtgggt cgatccgcag atggcgcatg gctcatccca
    37441 ctgctgaacg gtgaacgggt gatcgtcgaa atcgaggctt tccacgcggg taaacggcgg
    37501 caccgcatag atgcgcttct cacgcccggc gccgaagagt tgcagggcgg gcatcatgtc
    37561 cattttcggg ttatcgaatt tcgggatcgg cgacgggtcc attacgtagc gcccgttcac
    37621 cttcaccgga taggcgtagg tggtggcgat atgaccgaag cgggcgatat cttcatacag
    37681 cttcacctgc atcacgccgt attcctccag cgcgtgcatg gtgcgggttt ccgtttcgcg
    37741 cggctcgata aagcgcagcg gttcggggat cggcacctgg aaaataatga tctgatcttc
    37801 ggtcagcggc gtttcgggga tgcggtgacg cgtctggatg accgttgcat cgtccgtgcg
    37861 ctcggtggtg tttaccccgg tcacgcgctt aaagaagttg cgaatcgaca cggcgttggt
    37921 ggtgtcatcc gcgccctggt caatcacctt cagcacgtcg ctttcgccaa tcacgctggc
    37981 ggtaagctga atgccgccgg ttccccagcc gtacggcatt ggcatctcgc gcccgccaaa
    38041 cggcacctga taaccgggga tcgccaccgc ttttaagatg gcgcggcgga tcatgcgttt
    38101 ggtctgctcg tcgaggtagg caaagttgta gccgctcaga ttagccatgg ttctgctcct
    38161 gttgcagacg tttgagtagc tccagttcgg cctggaaatc gacgtagtgc gggagtttga
    38221 ggtgcgagac aaagcctgcg gcttcgacgt tgtcggcatg tgccagcacg aactcttcat
    38281 cctgcgccgg gcctgtcgcg tgctcgccgt actccggggc ttgcagagcg cggtcgacca
    38341 gcgccatcgc catcgctttg cgctcgctca tgccgaacac cagcccgtag ccgcgcgtga
    38401 agtgcggaga ttcatccggt gggtcgataa aaccgttgac catttcacac tcagtcatca
    38461 gcagttcgcc gacgttcacc gcaaatccca gctcttcggg cacaatcgac acgtcgatgt
    38521 aaccactgcg gatttcaccc gcgaacgggt gattgcgccc gtaaccgcgt tgggtggagt
    38581 aggccagcgc cagcaaatag ccttcgtcgc cgcgcatgag ctgttgcagg cgggaagaac
    38641 gtgagcaggg gtaaaccggt ggcgtgcggg tgatgtcatc cggctgtgcg ccgctatcct
    38701 cttcaaactt cgccagcccc tgacgcgcca gcaggctgaa aacgtgcggc gacggctgct
    38761 gttcgctgtc ggcggtggtc agcgtcggcg cttcgccgtt tgccagcagg gtgaaatcga
    38821 gcaggcgatg ggtgtagtcg taggttgggc caagcagctg gccgccggga atatctttat
    38881 aaaccgctga aatacgccgt tcgagacgca tctccgtggt gtcgagcggc tcgcttaccg
    38941 cgagcttcgc cagcgtggtg cggtaggcgc gcagcaggaa aattgcttcg acgttatcgc
    39001 cgctggcctg tttcagcgcc agcgccgcca gctcgcggtc ggcaatgccg ccttcggtca
    39061 tcacgcgatc caccgcaaga ttaagctgct gttcaatctg ggcgacgctc agttcaggca
    39121 aatcggtatc gccccggcgt cggctctctt gcagggcgtg ggcggcgtcg atcgccttct
    39181 cgcccccttt cacggcaacg tacatcagca cacctccaca tgagtggttc gcggaacagc
    39241 cagcaggcgc tcgccgcagg tcaggatcag gtcgatgccg agcgggaacg ggtgcgggcg
    39301 ctcggtgagt tcgtgcagaa tgcactccgg cagctgcggg gcgatcattc gttcttcggc
    39361 aatacccgcg ccggtgagac gcagcatgcg cccgccgctc aggctggcga cctgtaaaat
    39421 cagcgttgcg cccgcttccg gcgcaacggc ggtgccggtg gaaagggcgt taagctgttc
    39481 gctggaaatc gcctcatccg ttaccgcgaa ggtcgcctgt tccggctggc tgaccagcgg
    39541 cgcgttggta tgaaaacgca ggctctggct gacgatatcg ttgctcaatg gcgcggcaag
    39601 ccacaccggc gtgtcgttat cggccagcgt cagcagcacg ctggtggtgg cgatattcag
    39661 cggctgccag ccgcgtttga gctgatgcag ggcgacaatc acgcccggct cgctcatggc
    39721 ctttaacagg cgacgaaaac tgtgctgggc atcctgcacg ggaagcataa aagcggtttc
    39781 cagggtcatg cgttgtctcc gcgaaccatc gtaaagaagt cgacccggct ggcgttcact
    39841 tcggcctggc gtgcggcaat gcgtgccata cggtcagcgt ccagcggggc aataagcgtt
    39901 tctgataagt tttgaaaatg gcgggtttgc tgcatcagcg cgtcaatcag cgcgcagcgt
    39961 tcggcgtgct gtttgtcgcg ccccagcacc cagctgtagc cgagggtgcc gtcagtcaga
    40021 cgtacggcgg cgcgggtcag cgtggcgtcg ccggcaaaaa agcgttcgcc ggtgccgccc
    40081 atccgcgcct gaatctgtac caggccagtt tcagcggcgc ggatcacctc atagttggcg
    40141 gtgatgttca gcgcgttcag gcgggctgcc agttctgccg gttggctgtg tgccagtacg
    40201 gacatccagt gctggcgggt cgcggtatct gcgtgcattc agtgctccat agtgaattca
    40261 atcatgtcgg cgcgcgtcag gctgacggag tactccgccg ggctgctttc accgtcacgg
    40321 tggttaaggg tgcgcacgca cagcagcggg gacatattcg gaatttcaag acgctggcac
    40381 tctttggcct gggcgcggcg ggcgctgatc cgcgtctggc tgcggcgcag tgcaatcccc
    40441 gtttgctcgc gcagaaaatc gtgcagcgag ccgctgtcga agcgttgcag cgtcggccag
    40501 agggtgaggt ccgcgaagta gtggtcgatt aaacagagcg cgatgccgtt gacccggcgc
    40561 agggtgcgca gatggatgac gttctccccc tcggttatcc ccagcgcgtc agcgacgtga
    40621 ccggaggccg ggcgcagcac agacagcagt ttttcgctgg tgggatggct gccctgatcc
    40681 agcagattct ggctaaaacg cgcctgggcg ttgagcgggt aatcgaacgg gcgcatcagc
    40741 accagcacgc cgacgccctg acggcgctgt acccagcctt tttccaccag ttggtcgatg
    40801 gcgcggcgca gggtgtggcg atttacctca aagcgcgccg ccagctgctg ctcggcggga
    40861 agatagtcgc cgcagcggta gtgttgacga agctcctgct caagttttgc ggctatctcc
    40921 tgatagcgtg ttgggtagct ggtcggatgt gtagacaagt gcatagctat caatgcctcg
    40981 ctaatcagat aaagtgctta cgcaaccgtt gagagaggaa atccagcagg ctgaccgtga
    41041 cgataatcag caccatcagg gcgcaggttt gctggaactg gaaaccgcga atcgcttccc
    41101 acagcgtgac gccgatcccg cccgcgccga ccatgccgac gacggtcgcc gagcggacgt
    41161 tggattcgaa gcgatagagg gagtaggaga tcagcagcgg catcacctgc ggcagcacgc
    41221 cgtagaggat ctcttcgagc ttgttggcgc cggtggcgcg aatgccttcc accgggccgg
    41281 gttcaatcgc ttccaccgct tcggaaagca gtttggagag cacaccggtg gtgtggataa
    41341 acagcgccag cacaccagcg aatgggccga ggccgacggc gaccacgaac agcatggcga
    41401 agaccatttc gttaatggcg cggcaggcgt ccatcaggcg gcgcacgggc tggtaaaccc
    41461 accacggcac caggttttcg gcgctcatca ggccgaaggg gatggagaga accaccgcca
    41521 gcgcggtgcc ccagacggcg atttgcagcg tgacgagcat ttcggtgagg taatcctgcc
    41581 actggctgaa atcgggcggg aagaagtcgg cggcgaaggt cgccatgttg ccgccgtctt
    41641 tgatcagcgt gagcggggcc atttccgcgc cctgccacga gacgaccaac acggcgagta
    41701 caacggccca gctcagaagc gagaaccagc tgcgcttggg tggggcgatg gtgatggttt
    41761 gcatgtttgg ctccggtctg taggccggat aaggtgcttg caccgcatcc ggcatcaacg
    41821 cctgcacatt gtcggatgcg acaccggggc gtcttatccg acctacgatt tactgcatcg
    41881 ctttacttac cgaactcatc gcgcttaacg cgttgttcag gcggtccagg tcatccagct
    41941 gcgcttgaat ctcggtggtt tttgccagct tgtcctgctc attcagccct ttattgcctt
    42001 tcacgctttg catctcttta aacagtgcga gctggcgaat cggcaccagt tgcaggtcgc
    42061 tggaggcgcg gaacggcgcc cagcccaggc gttccagcac cgctttctct tccggcgtct
    42121 tgccgtaatt cataaagaag tcgtagatct tgtctttggt ggtttcggaa aggtttttac
    42181 gccagacgat cggatcgcct gggatcagcg gtgacttcca gatcactttc agttctttca
    42241 gcttctccgg cgcggaggtt ttcagcttgt cgaggttttc ggtgttgttg gtcgccacat
    42301 ccacctgctt gttggcgacg gccagcgcgt tggtttcatg cccggcgttg acggtgcgct
    42361 tgaagtcact ggcggagata ttgtttttgg agaagacgta gtagccgggg acgaggaagc
    42421 cagaggtgga gttaggatcg ccattaccga aggtgagatc tttccgcttc gccagcagat
    42481 cgttcaggtt gttgatcggg ctgtctttgt tgacgatcaa cacgctccag taacccggcg
    42541 atccatccgc cgcgacggtc tgagcgaaga cttggccgtt ggcgcgatcc actgcttcca
    42601 tcgccgacag gttgccgtac caggcgatat ccactttgtt gaaacgcatt ccctggataa
    42661 tgcccgcata gtccggggca aagaaggcgt tgaccttcac gcccagcttc ttctccatat
    42721 cctgtaaaaa cggcgtccat tgcggtttca ggttttgctg tgattccgtt gaaataatgc
    42781 cgaaattcag cgccttttcc tgctcttcgg cgtgtgccgg gcttaacagg gtgctgaggc
    42841 tgaacatgct ggtgaaggcc agcgaggcaa ttatcttagc gttcattcgt tttcctcaat
    42901 gatggggatg tcaggcagct ttcgcgttct cttcgacgcg gttgatgctg cggtagagat
    42961 ggtcaaaacg ttcgttatca aactgttggc tgctgccgtc gtagaagacg tgcccctggc
    43021 gcagggcgac gatgcgttcg cagtagcgca gggcgtaatc cacctgatgc agcgtgacga
    43081 ccacggtgat gccgtcgttc tggttgatgt cgcgcagggt gtccatcacg atgcgcgccg
    43141 attccgggtc cagcgaggcg atcggttcgt cagcgagaat cactttcgcc tgctgcatca
    43201 gcgcgcgggc aatcgccaca cgctgctgct ggccgccgga gagcgtggaa acacgctgat
    43261 acgcaaagtg cgccataccg acgcgggtca gcgcctgtaa tgcgcgctgt ttctgctcgc
    43321 gggtgaacca actaaaacag gtgcgccaga acggcgtgct gccgagcgcg ccaatcagca
    43381 cgttctccag tacgctcagg cgattcacca ggttgaattg ttggaagatg tagccggtgt
    43441 tggcgcggct tttgcggata tcgcgggcca ggcggccttc gcgctggaca gtgcggccca
    43501 gcagctcgat atggctaccg gcggatttat cgccggtaat caaaccgctt aagtgacgta
    43561 aaagggtgga tttgccggaa ccggacggcc caagcagagc caccatttca ccatgatgga
    43621 tattcagatc aaccgcgtgc agcgcctgat gctggttgaa ggttttgctc agttgttcga
    43681 cgcagataat cgtttgcatg atgcagcctc cctaaaaaag tggctccatc gtggcggatt
    43741 attgtgacga tttggttaat taaaagtgac taacagatga agagttaacg ggaattcgat
    43801 gacagtgcgg gagggcgggc ctcccggcgg attattgcgt tggttgttgt ttgacgacat
    43861 taatcatcca cggtacgcca aatttatcgg tgactttgcc aaagccatgc gcccagaaag
    43921 tttcctgcca ggccatttcg atttttccgt tagcggcaag gttatcaaac cagcgttttc
    43981 cttcttcgac ctgttgcgaa tcgagcacca gcgtaaaacc ggagtagctg gcttttcctg
    44041 acggtatagc atcgctcatc atgatatcgc ttccggcaat gcgcacattg gcgtgggcga
    44101 tggcggtatc gggaaattgc attccggaag ggcagttctc ggcgctgtcc tgcgctgatt
    44161 ttggcatttc gccgaagctg attttataga gcagttcagc gcccaacgta cgttgataat
    44221 aggcaatcgc gtcggagcag ttaccggcaa aagagaggta gggacttaac ggcatgatgg
    44281 tgacctcagt taagagaagc cagttaagtg tagttcgaaa tttatacaga tgagagggaa
    44341 gcctgataag acgcgcgagc gtcgcatcag gcagtcggca ctgttgccgg atgcggcgta
    44401 aacgccttat ccggcctacg gagggtgcgg gaatttgtag gcctgataag acgcgcgagc
    44461 gtcgcatcag gcatctggca ctgttgccgg atgcggcgta aacgccttat ccggcttacc
    44521 gatcacaata caatcagttc tttttcacaa actcagattt cagtttcatc ggaccaaaac
    44581 cgtcgatttt gcaatcgatg ttatggtcgc cttcaaccag gcggatgttt ttcactttgg
    44641 tgccaatttt cagcatcgaa gagctacctt tcaccttcag atctttaatg atggtaacgc
    44701 tgtcgccgtc agccagcaga ttgccgttag catctttaac gatcagctcg tcgctttcct
    44761 gtgcaggttc tgcgtcgttc cattcgtagg cacattccgg gcagatgtac atgccgttat
    44821 cttcgtaagt gtattcggag ttgcattttg ggcagtgtgg taatgacatg tggatttcct
    44881 caaaagtcag cagggcaaaa gcgccaaaaa acggcagatt gccgaaaaag gccgcaatta
    44941 tacacaaaat ccctgagttt gtcgggggta ctgctgattt tagacgacga tcgttttcat
    45001 ttgaaaggaa acgcaaaaag aaaataatct ttttgcctcc atttattatt taagataagg
    45061 ttaatgctct acttgttc
//
LOCUS       DAZKIP010000023        43327 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZKIP010000023 DAZKIP010000000
VERSION     DAZKIP010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.023"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            14..916
                     /locus_tag="ACHOUK_001548"
     CDS             14..916
                     /locus_tag="ACHOUK_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9897021.1"
                     /translation="MTNLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLRGLEQTINIVNKSELKKNFII
                     HGPQLISCSNNSMLIRCLRQDSSVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNRHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDER
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNECF"
     gene            1063..2283
                     /locus_tag="ACHOUK_001549"
     CDS             1063..2283
                     /locus_tag="ACHOUK_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFP9897022.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            2256..2885
                     /locus_tag="ACHOUK_001550"
     CDS             2256..2885
                     /locus_tag="ACHOUK_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFP9897023.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRINAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(2886..4046)
                     /gene="ybdL"
                     /locus_tag="ACHOUK_001551"
     CDS             complement(2886..4046)
                     /gene="ybdL"
                     /locus_tag="ACHOUK_001551"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFP9897024.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQASAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     IADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTREHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            4155..5243
                     /gene="hcxA"
                     /locus_tag="ACHOUK_001552"
     CDS             4155..5243
                     /gene="hcxA"
                     /locus_tag="ACHOUK_001552"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFP9897025.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGVR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(5253..5450)
                     /locus_tag="ACHOUK_001553"
     CDS             complement(5253..5450)
                     /locus_tag="ACHOUK_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFP9897026.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(5632..7737)
                     /gene="cstA"
                     /locus_tag="ACHOUK_001554"
     CDS             complement(5632..7737)
                     /gene="cstA"
                     /locus_tag="ACHOUK_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFP9897027.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVVQAKGAH"
     gene            complement(7918..8331)
                     /gene="entH"
                     /locus_tag="ACHOUK_001555"
     CDS             complement(7918..8331)
                     /gene="entH"
                     /locus_tag="ACHOUK_001555"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFP9897028.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDI
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(8334..9080)
                     /gene="entA"
                     /locus_tag="ACHOUK_001556"
     CDS             complement(8334..9080)
                     /gene="entA"
                     /locus_tag="ACHOUK_001556"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFP9897029.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(9080..9937)
                     /gene="entB"
                     /locus_tag="ACHOUK_001557"
     CDS             complement(9080..9937)
                     /gene="entB"
                     /locus_tag="ACHOUK_001557"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFP9897030.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9951..11561)
                     /gene="entE"
                     /locus_tag="ACHOUK_001558"
     CDS             complement(9951..11561)
                     /gene="entE"
                     /locus_tag="ACHOUK_001558"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFP9897031.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGE
                     HNLQDAINHPADGFTATPSPADEVVYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQINVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(11571..12746)
                     /gene="entC"
                     /locus_tag="ACHOUK_001559"
     CDS             complement(11571..12746)
                     /gene="entC"
                     /locus_tag="ACHOUK_001559"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFP9897032.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12935..13891
                     /gene="fepB"
                     /locus_tag="ACHOUK_001560"
     CDS             12935..13891
                     /gene="fepB"
                     /locus_tag="ACHOUK_001560"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP9897033.1"
                     /translation="MRLAPLYRNALLLTGLLFSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSTEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQALLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(13895..15145)
                     /gene="entS"
                     /locus_tag="ACHOUK_001561"
     CDS             complement(13895..15145)
                     /gene="entS"
                     /locus_tag="ACHOUK_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFP9897034.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDG"
     gene            15256..16260
                     /gene="fepD"
                     /locus_tag="ACHOUK_001562"
     CDS             15256..16260
                     /gene="fepD"
                     /locus_tag="ACHOUK_001562"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFP9897035.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            16257..17249
                     /gene="fepG"
                     /locus_tag="ACHOUK_001563"
     CDS             16257..17249
                     /gene="fepG"
                     /locus_tag="ACHOUK_001563"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFP9897036.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            17246..18061
                     /gene="fepC"
                     /locus_tag="ACHOUK_001564"
     CDS             17246..18061
                     /gene="fepC"
                     /locus_tag="ACHOUK_001564"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFP9897037.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTLGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLRELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(18058..19191)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHOUK_001565"
     CDS             complement(18058..19191)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHOUK_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFP9897038.1"
                     /translation="MSSLNIKQGSDAHFPDYPLASPSNNEIDLLNLISVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKMKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(19407..23288)
                     /gene="entF"
                     /locus_tag="ACHOUK_001566"
     CDS             complement(19407..23288)
                     /gene="entF"
                     /locus_tag="ACHOUK_001566"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFP9897039.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSELPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEQTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLAGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAAFWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQHYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYQFSYREMREQVVALANLLREHGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFVPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMAPVVLLQLPQLPLSANGKLDRKALPLPELKAQTPGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPLWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFEKIGPIIRATLNR"
     gene            complement(23285..23503)
                     /locus_tag="ACHOUK_001567"
     CDS             complement(23285..23503)
                     /locus_tag="ACHOUK_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family protein"
                     /protein_id="HFP9897040.1"
                     /translation="MAFSNPFDDPQGVFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(23506..24708)
                     /gene="fes"
                     /locus_tag="ACHOUK_001568"
     CDS             complement(23506..24708)
                     /gene="fes"
                     /locus_tag="ACHOUK_001568"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFP9897041.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLISL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            24951..27191
                     /gene="fepA"
                     /locus_tag="ACHOUK_001569"
     CDS             24951..27191
                     /gene="fepA"
                     /locus_tag="ACHOUK_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFP9897042.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDNDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGITWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            27366..27986
                     /gene="entD"
                     /locus_tag="ACHOUK_001570"
     CDS             27366..27986
                     /gene="entD"
                     /locus_tag="ACHOUK_001570"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFP9897043.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(28108..28260)
                     /gene="hokE"
                     /locus_tag="ACHOUK_001571"
     CDS             complement(28108..28260)
                     /gene="hokE"
                     /locus_tag="ACHOUK_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFP9897044.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(28611..28763)
                     /gene="hokE"
                     /locus_tag="ACHOUK_001572"
     CDS             complement(28611..28763)
                     /gene="hokE"
                     /locus_tag="ACHOUK_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFP9897045.1"
                     /translation="MLTKYALVAVIVLCLTVPGFTLLVGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            28732..28999
                     /locus_tag="ACHOUK_001573"
                     /pseudo
     CDS             28732..28999
                     /locus_tag="ACHOUK_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            29202..30320
                     /locus_tag="ACHOUK_001574"
     CDS             29202..30320
                     /locus_tag="ACHOUK_001574"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFP9897046.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHKEKD
                     YLLYKFNRFQACRYGLEGVITDPYTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            30386..30634
                     /locus_tag="ACHOUK_001575"
     CDS             30386..30634
                     /locus_tag="ACHOUK_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFP9897047.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTTFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            30699..31067
                     /locus_tag="ACHOUK_001576"
     CDS             30699..31067
                     /locus_tag="ACHOUK_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFP9897048.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            31161..31814
                     /gene="nfsB"
                     /locus_tag="ACHOUK_001577"
     CDS             31161..31814
                     /gene="nfsB"
                     /locus_tag="ACHOUK_001577"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFP9897049.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            31922..33169
                     /gene="ybdG"
                     /locus_tag="ACHOUK_001578"
     CDS             31922..33169
                     /gene="ybdG"
                     /locus_tag="ACHOUK_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFP9897050.1"
                     /translation="MQDLISHVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(33237..34613)
                     /gene="pheP"
                     /locus_tag="ACHOUK_001579"
     CDS             complement(33237..34613)
                     /gene="pheP"
                     /locus_tag="ACHOUK_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFP9897051.1"
                     /translation="MKNASNVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLAMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(34715..37852)
                     /gene="cusA"
                     /locus_tag="ACHOUK_001580"
     CDS             complement(34715..37852)
                     /gene="cusA"
                     /locus_tag="ACHOUK_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFP9897052.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKIQVGPEMRRGIA
                     ELNGEVVGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRAID
                     NLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMSLG
                     GIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITNASVEVGPALFI
                     SLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGKIP
                     PESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQINEG
                     DLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMVET
                     TIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIGIK
                     VSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTVAD
                     VQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITLAD
                     VADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSVAF
                     SGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWLLW
                     WMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEALH
                     HGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFIIP
                     AAYKLMWLHRHRVRK"
     gene            complement(37864..39087)
                     /gene="cusB"
                     /locus_tag="ACHOUK_001581"
     CDS             complement(37864..39087)
                     /gene="cusB"
                     /locus_tag="ACHOUK_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFP9897053.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGAPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(39103..39435)
                     /gene="cusF"
                     /locus_tag="ACHOUK_001582"
     CDS             complement(39103..39435)
                     /gene="cusF"
                     /locus_tag="ACHOUK_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFP9897054.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGFDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSGIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(39459..40841)
                     /gene="cusC"
                     /locus_tag="ACHOUK_001583"
     CDS             complement(39459..40841)
                     /gene="cusC"
                     /locus_tag="ACHOUK_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFP9897055.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALENNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTVLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            40998..41681
                     /gene="cusR"
                     /locus_tag="ACHOUK_001584"
     CDS             40998..41681
                     /gene="cusR"
                     /locus_tag="ACHOUK_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFP9897056.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            41671..43119
                     /gene="cusS"
                     /locus_tag="ACHOUK_001585"
     CDS             41671..43119
                     /gene="cusS"
                     /locus_tag="ACHOUK_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFP9897057.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVFLLSGPTMMMPGHGHGHMEHSNWRMISLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELQFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHLVQVIVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSNARGTRFVIVLPERG"
BASE COUNT        10436 a        11943 c        10949 g         9999 t
ORIGIN      
        1 accggagtag tgtatgacca atctctacga cttgaaaaag ttcgacttaa atcttcttgt
       61 catatttgag tgtatttacc agcatttaag cattagcaaa gctgctgaat cactatatat
      121 caccccatca gccgtgagcc agtcgttaca acgcttacgt gcgcaattta atgacccttt
      181 attcattcgc tcaggaaaag gtatcgcgcc gacgacaaca gggttaaatc tgcatcacca
      241 tcttgagaaa aatctcagag gccttgaaca aacaattaat atagtcaata agtccgaact
      301 aaagaaaaac tttattattc acggccctca gctcatttct tgctctaata atagtatgct
      361 gatccgctgc ctacggcaag attcctctgt cgagattgag tgccatgata tcctcatgtc
      421 agcagagaat gctgaagaac tgttggttca ccgcaaagcc gatctggtga tcacccaaat
      481 gccagtgatc agccgttccg tcatttgcat gccattgcat acgattcgca atactctaat
      541 ttgtagtaat aggcatccac gaattactga caatagcact tatgaacaaa ttatggcaga
      601 agagttcacc caattaatat ctaaatcagc aggggttgat gatattcaaa tggaaataga
      661 cgagagattt atgaatcgca aaatttcatt tcgtggttcc tctttgctga caatcattaa
      721 tagcattgca gttacagatt tactgggaat agtcccctat gaactttata actcccaccg
      781 tgactttctt aatttaaaag agataaaact agaacatccg ttaccttcaa taaagcttta
      841 catttcctat aataaatctt ctttaaacaa tctggtgttc tcacgattta ttgaccgtct
      901 aaatgagtgt ttttgatata taacctcaga cagctactat aactttcacc tatttattca
      961 cagcgcgaat gaaaaataaa acactctgct tttcagtgtt tactttataa ttaacagtct
     1021 tatctcactg atcaccctgt aacgttcaga gagcgtcttc agatgtctat ttataaaatt
     1081 cctcttccgc tcaatattct ggaagctgca cgagagcgta ttacctggac actcaacacc
     1141 ctaccccgcg tttgtgtctc attttctggc ggcaaagact ctggtttaat gctgcatctg
     1201 acagccgaac tcgcccgaca aatgggcaaa aaaatctgcg ttttgtttat cgactgggag
     1261 gcgcaattct cttgcaccat taactatgtt cagtccctgc gcgagttgta caccgatgtc
     1321 atcgaagagt tttactgggt tgcgctcccg cttacgacgc aaaattccct ttcacaatac
     1381 caacccgaat ggcagtgctg ggaacctgat gtcgaatggg tacgtcagcc cccgcaagat
     1441 gcgataaccg atcctgactt tttctgcttt taccagccag gcatgacctt cgaacaattt
     1501 gtacgtgagt ttgccgaatg gttttcacaa aaacgtccgg cggcgatgat gatcggcatc
     1561 cgtgcggatg agtcctacaa ccgttttgtc gccatcgcca gtttaaataa acaacgtttt
     1621 gccgacgata aaccctggac tacggccgca ccaggcggtc atagctggta catttacccc
     1681 atttacgact ggaaagtggc tgatatctgg acctggtatg caaatcatca aagtctctgc
     1741 aacccactgt ataacctaat gtatcaggca ggcgttcctc tgcgtcatat gcgaatttgc
     1801 gaaccttttg gcccggagca acgacaagga ttatggctct atcacgttat cgaaccggat
     1861 cgctgggctg ctatgtgcgc acgagtcagc ggggtaaaaa gtggcggcat ttacgccgga
     1921 catgataatc atttctatgg gcatcggaaa atcctcaagc cagaacattt agactggcaa
     1981 gaatatgcat tattgctgct caatagcatg ccggaaaaaa cagctgagca ttaccgcaat
     2041 aaaatcgcca tttatttgca ctggtatcag aaaaaaggca tcgaggttcc acaaacccag
     2101 caaggggaca ttggcgcgaa agatatcccc tcctggcggc ggatatgcaa agttttactc
     2161 aataacgatt actggtgtcg ggcattatca tttagtccta cgaaagcgaa gaactatcag
     2221 cgttataacg aacggataaa aggaaaacgt caggaatggg ggatactatg caacaacgat
     2281 taacgcagga tttaacgcaa tttctcgcca gcttgccgga agatgatcgc atcaacgcga
     2341 ttaatgaaat ccgcatggcg atccatcagg ttagcccctt tcgtgaagag ccagtggatt
     2401 gcgttctgtg ggttaaaaac agccaactta tgcctaatga ttacaaccca aacaacgtgg
     2461 caccacccga aaaaaaactg ctgaaaaaat ccattgaaat agatggtttt acccaaccca
     2521 ttgtggtcac gcatacggat aaaaatgccc tggaaatcgt ggatggtttt caccgccatg
     2581 aaattggtaa aggctcatca tcattaaaat tacgcttaaa aggttatttg ccggtgacct
     2641 gtctggaagg tacgcgtaat caacgcatcg ccgcgactat tcgtcataat cgcgctcgtg
     2701 gacgccatca aatcaccgcc atgtcggaaa tcgtccgtga actcagtcag ttgggatggg
     2761 acgataataa aattggcaaa gagctgggaa tggacagcga cgaagtgctg cgcctgaagc
     2821 aaattaacgg tctgcaagag ctgtttgccg accgtcaata ttcccgcgcc tggactgtca
     2881 aataactaca gctggcgcag tcgttcagct gctgccagca acgtcgattc cttcttggca
     2941 aaacagagac gaatcagttt atgtgggaag ggatcggcgc aaaacaccga tagcggaatc
     3001 gccgccacgc cgtgctcccg cgtcagccac tggcaaaact caacatcatc cagggtagaa
     3061 accgcgctgt aatccaccag caaaaagtat gtgccttcgc acggtaaaat ctccagccga
     3121 ctttcattta aggcatttac cagaatatcg cgcttctggc gataaaagtc cggtaacgca
     3181 agataatgct caggttctgc acgtagcata tccgcaattg ccagctgtgc cggggtattc
     3241 accgaaaagg tcagatactg atgtaccttg cgaatttcgg cgctgatggg cgctggcgca
     3301 acacaataac ccactttcca gccggtcata tgataggtct tgccaaacga tgacaccgcc
     3361 accgcccgct cacgcagctg cggatgcgcc agcacactgg catggccctg ttgtgaaaag
     3421 ttgatgtgct cgtagacttc atcgctaatg acaaaaatct cgtgcccggc gctcgcctgc
     3481 cacaaagcgg cgaaatcagc ctgctgccag acagttgcac tgggattatg cggagtgttg
     3541 aggatcacca gtctggtgcg ctcgcttaac aatgcggcaa attcctgcca gtcaacgcga
     3601 aaatgcggtg gttgcagtgc catacgcttc actattcccc cagaaagcgc gatggcgggg
     3661 gcgtaactgt catagctggg atcaaaacaa atcacttcat cgccattgcg caccagtgcg
     3721 gtaatcgccg cgtataacgc ttccgtcgcc cctgccgtta cggtgatatc gctatcggca
     3781 tctggttgat agccatacaa acgttccgtt ttctgagcaa tcgcctcgcg caaggcctgc
     3841 acgccggtca tgggcgcgta ttggtttgcc ccctgggcaa cgtggtacgc cagccgctcc
     3901 tgtaaatagc gcggaccatc aaaatcagga aagccttgcg acaggttaat cgcctggtgt
     3961 tgctgcgcca gcgcgctcat ctgggtgaaa atagtggtgc caagttgtgg aagtttgctt
     4021 tgtggaatca gagggttatt tgtcattttt attgtacctg acgaagttgc gtgttgaagg
     4081 cactataaca cgatgctagt atttggcaat caagacgttt agatgtctaa ataaaacaat
     4141 aaggacaaca caacatgcct cacaatccta tccgcgtggt cgtcggcccg gctaactact
     4201 tttcacatcc aggaagtttc aatcacctgc acgatttttt cactgatgaa caactttctc
     4261 gcgcggtgtg gatctacggc gtacgcgcca ttgctgcggc gcaaaccaaa cttccgccag
     4321 cgtttggact tccaggggca aagcatattt tgtttcgtgg tcattgcagc gaaagcgatg
     4381 tacagcaact ggcggctgag tccggtgacg atcgcagcgt ggtgattggc gtcggcggcg
     4441 gcgcactact cgacaccgcg aaagccctcg cccgccgtct cggtctgcca tttgttgccg
     4501 ctccaacgat cgccgccacc tgcgccgcct ggacaccgct ctccgtctgg tataacgatg
     4561 ccggacaggc gctgcattat gagattttcg acgacgccaa ttttatggtg ctggtggaac
     4621 cggagattat cctcaatgcg ccgcaacaat atctgctggc ggggatcggt gatacgctgg
     4681 cgaaatggta tgaagcggtg gtgctggctc cgcaaccaga aacgttgccg ctaaccgtgc
     4741 gactggggat caataatgct caggccattc gcgacgtctt gttaaacagt agcgaacagg
     4801 cgcttgccga tcagcaaaat cagcagttaa cgcaatcatt ttgcgatgtg gtggatgcca
     4861 ttattgctgg tggtgggatg gtcggtggtc tgggcgatcg ttttacgcgt gtagcggcgg
     4921 cccatgccgt acataacggt ctgactgtcc tgccacaaac cgagaagttt ctccacggca
     4981 ccaaagtcgc ctacggaatt ctggtgcaaa gcgccttgct gggtcaggat gatgtgctgg
     5041 cgcaattaac cggggcgtat cagcgttttc atctgccgac tacgctggcg gagctggaag
     5101 tggatatcaa taatcaggcg gagatcgata aagtgattgc ccacaccttg cgcccggtgg
     5161 agtccattca ttacctgcca gtcacgctga caccagatac gttgcgtgca gcgttcgaaa
     5221 aagtggaatc gtttaaagcc tgacgtgcaa atttagcagc agcgcgcgcc gccttttcca
     5281 ccgtagcgcg cgtcctgccg ctcacggaaa aactcttcgt aggtcatcgg cgtttgatcg
     5341 ggatggttaa cccgcatatg ttcgacatag ttgtcgtaat caggcatacc aatcatcagc
     5401 ttcgccgcct gacctaaata ttttccggct tttgccagtg aatcaaacat cttggcctcc
     5461 gctaaccatg ttgctgaaag agtgccggat gcggcgtgaa cgccttatcc ggcctacaaa
     5521 tgctatcaac gcgctcggtc agccccctcg ccccatcggg gagagggtta gggtgagggg
     5581 gaacccacgg cgccatgcca acattcgcca acatcccccc tcactctgac tttagtgcgc
     5641 gccttttgcc tgcaccacga tctcctcgac attttccggc attggctcat acggcgtttc
     5701 tttcgccgtt ggcttcggct ctttcaatgc cgcaagtgcc gtcttaatcg agaacagtgc
     5761 cagaaccacc acgaccacca taaagaagat ggttaacccg gcatccagac ggttgttgaa
     5821 caccagttgc gccagctgtg actcagtata ctgcgatgga atattaccgc tgtcgatcat
     5881 tgcctggaac ttattagcaa tggccaggaa gccgactttc gcatccgggc taaacgcttt
     5941 ctgccagcct gcggtcaggg tacaaatcag cagccaggcc gttggtacca gcgccaccca
     6001 ggcgtaacgt tgacgtttca tcttgaacaa caccacggca cagagcatca gcgccatccc
     6061 tgccagcatc tggttggcaa taccaaacag cggccacaga gtgttaatac cgcccagcgg
     6121 atcgaccaca ccctgatgca ggaagtagcc ccacgccagc acgcacagcg ccgttgccag
     6181 caggttagca ggcagtgaat cggtccgttt caggcccgga gaaaccacgc ccagcagatc
     6241 ctgcaacata aagcgcgcag cacgcgtacc tgcatccacc gccgtcagaa taaacagagc
     6301 ttcaaacaga atggcgaagt gataccagaa cgccacatcc atcatgccgc ccagcgcgcc
     6361 atgcagaatg taggccatcc ccaccgccaa cgtcggcgca ccgcccgcac gggaaatgat
     6421 cgactgttct cccacttcgc tggcaatctg gttgagcgta tccggagtaa tcgcaaagcc
     6481 ccagctactc accacttgcg cggcagaagc gaccacatcc gccgtccctg ccggagccag
     6541 caccgccatc gggctgttca tggcgaagta cacgcccgga tcgatgatac aggcggaaac
     6601 cagtgccata atcgccacga aggattccat taacattcca ccgtagccga taaaacaagc
     6661 ctgcccttcg ttcgccagca tcttcggcgt ggtgccggaa gagatcagcg catggaagcc
     6721 agacaccgcg ccacaggcga tggtgataaa caggaacggg aacaggttac cggtccatac
     6781 cgggccagtg ccgtcaacaa atttggtcaa cgcaggcatg gtcagcgtcg ggcgcataat
     6841 caaaatgcct accgccagac caacgatcgt cccgattttc aggaaggtag agaggtagtc
     6901 acgcggagcc agcagcaacc ataccggcag caccgccgcc acaaaaccgt aacccaccag
     6961 catccaggtc agctgcacgc cggtaaagtc aaagtacggt gcccaggtcg gactttctgc
     7021 cacccagccg ccagaaataa tggcgaaaat caggaatacc agaccgatga ccgacacttc
     7081 accaatacgc cccggacgca gatagcgcag gtagatcccc atgaacagcg ccagcggaat
     7141 ggtgaacgca acggtatacg ttccccacgg gctatgagtc agggctttca ccacgatcat
     7201 cgccagtact gcaaggataa tgaccatgat cataaagcag gccaccagcg cgatcacccc
     7261 ggcggttggc cccatctctt ctttgaccag ctcacccagc gaacgaccgt cacggcgcgt
     7321 agaaacaaac agcaccatga aatcctgcac cgcaccggcg agaaccaccc cggcgagtag
     7381 ccagatcatc cctggcaggt aacccatttg cgccgccagt accggcccaa ccaacggacc
     7441 tgctccggca atggccgcaa aatggtgacc gaacagcact ttcttgtccg tcggcacata
     7501 gtccagcccg tcgttatggc gcaccgctgg cgtcatacgc gtcgggtcaa ccgccagcac
     7561 atttttggcg atatacagac cataaaaacg gtaagcgatc agatagatac agaccgacgc
     7621 caccacaatc cacagcgcgt tgatctgttc cccacgattt aaagcaatat atcccagagc
     7681 aaatgctccc attacagaga gcactgtcca gacgaggtat ttccctgatt tgttcatagt
     7741 tgttatccgt gtgcgtgtcc atagagatgt tacattttgt ttctataaca tctttgtacg
     7801 atttaacaac acagcaacca tgtaaatcgg agttgaaacc aatatttaac ttaacaatgt
     7861 taactcgatc actccgttaa ccgagttttc ctgcaatctc atataattct gtcgggttca
     7921 tcccaaaact gccgtaccca gccgacaagt gcagcaacgc cgcccctgtt catcgaaaac
     7981 gacgatttcc cagctctgat tttgccgccc aagatgcagc ggctggcaga cgccgcgtac
     8041 cttgccttca gacaccgggc gatgatgtgt ggcgttaagt tccgtgccca ccacacactg
     8101 cccgtcgcgg gtcatcataa atccggccat cgatcccagc gtttcagcca gcgccgccga
     8161 cgcgccgcca tgcagcaggc caaacggctg atgagtacgg atatcaaccg gcatttcggc
     8221 ttccagcaca tcatcgccca gacgggtata cacaattccc agatgcgcca ccattgtgtt
     8281 atcgctggtg gcgttcagtt cgtcgagcgt taaatggcgt ttccagatca tgcttatgcc
     8341 cccagcgttg agccgccatc gaccacaata tcctgtaggg tgatatggct ggcgaggtca
     8401 gaggcgagga acaaaatcgt gttggcgatc tcttgtggac gggcgatttt ccccagcgga
     8461 atgccgagtt taaactgctc gccaaagccg cgaatacgct gttcttcggc gtcatcgctc
     8521 acccacagcg tgcgttgcat atcggtatcg gtggaacccg gcgaaaccac attacagcgc
     8581 acgccgctac ccgccagttc cagcccgacg ctcaacgcca ggcttttcag cgccgctttc
     8641 gatgcgccat aagcactcat accaatacgc ggcgtgtgtg cggcgtcgga cgccacagtg
     8701 acaatcgccc cgccccgctg acggcgaaac tggttcatgg tttgctggaa caggttaaac
     8761 gcaccgccga cattaaccgc aaaagtctgc tgccagtcct ccttactgag ctgatcggtc
     8821 gcgcccatgc gcaaaattcc cgccgcattg accagcacgt ccagtcgctc cgtttcagct
     8881 aacagtcgct gacatacctg cgccacctgc gcagcatcgg caacatccat tacttcggtc
     8941 gcaaagggat attgctcctg agcgaacgct tgatcaaaac ctgtaacttt cgctcccgct
     9001 tcaacaaacg ccagcgccgt ggcgtagccg atacctttcc ccgcgccggt tacccagaca
     9061 tttttaccgc tgaaatccat tatttcacct cgcgggagag tagcttccac caggcgtcga
     9121 tggtcgggtt tttcgccaac atgacaaagt cgatatcgcc atgcactttg cgccagcgcg
     9181 ccgccagcgc catcatgcgc accgaatcca gaccgtagtc gatcaggttg tcgtcatcga
     9241 acggttcatc ggactcgtcc agcaacggca ggatcacctc acgcagcgcc gctttgctgg
     9301 cggggatagg tgctggcagt aattcttcgg tcatcaccac ccggccagaa cgtccggcca
     9361 catatttcag cgacatcaaa tgctcgtcac ggctgaaatc ggccagcgca tccgccacca
     9421 taaacggttt aatatcgcgc ataaatgcgt cggttgcggt ggtcatacag ccaatgtggg
     9481 catatacccc ggtaataatc agctggttac gtccactctc tttgagcatt tgctccagcg
     9541 gagaacgatg aaacgcgctg tagcgccact tcaccagcac cgtgtcgtcg gcatctggcg
     9601 tcagtcgatc caccaccttt tgctgttccg gcgaacgggt caggcccggc ccccacatat
     9661 cattcaacag cgcccgatct tcatcgctct gctctttcgg ctgggcggtg taataaaccg
     9721 ggatattgtg ctgtttgcag tagtcgcgca gcgcagcaat attcgcgatc acctgctcca
     9781 tcatcgggca gttctcgccc cagaagctga caaaatagtc ctgcatatca tggattaaca
     9841 acgcggcacg ttgcggttca aaggcccagt caactttatt ctgcggaata tcgtgagact
     9901 ccggcagtgc gtaagcctgt aattttggaa tagccatcgt gttctctcct tcaggctgat
     9961 gcgcgtgacg ccagccactg acgtaattgt tttttatcga ctttcccgac cgccgtcagc
    10021 ggaagtgaat ccacacactc cacgcgatcc ggtaatttaa attcggcaat accctgttca
    10081 cgcaggaaac gacgcacctg caccgcgcga agcggctctt ttaccaccag ataagcacag
    10141 cttttttcgc ccatcagctc atcttccata ctgaccagtg cggcgtagat caccgccgga
    10201 tggcgcagta acaggttttc gatctcttcg gcagcgatct tctcgccgcc acggttgatc
    10261 tgatctttct cgcgcccctg cacggtgatg taaccctctg gatcaataga gatcagatcg
    10321 ccggaacagt aaaaaccgtt ggcatcaaag gcgctggcat tgtgctgcgg gcttttgtaa
    10381 tagccacgga aggtatacgg cccgcgcgtc atcagtcgtc cgacttcccc ttgcggcagt
    10441 ggatttcctt cggcatcggc aacccatact tcgtcatccg gacacattgg gtaaccctgg
    10501 gtatggataa ttttctccgc gctatcatca agacgggtat agttcaccag cccttccgcc
    10561 ataccaaaca cctgctgcaa ctggcaacca atctcagcgg gaatacgcgc cgcaagcgtg
    10621 gcagaaagac gtgcgccacc gacctgtaac agtttcagcg aggcaagctg cgcccggctt
    10681 tcgccttcgg tcagcgcctg caaccacagg ctgactgccg gcggcaccag cgcggtaacg
    10741 ttaatctgat gtttttcaat caatgggaag caaagcgtgg cgctgggatc ggccgccaga
    10801 acaaccgttc ctccggcaag aaagacgccc agcgatcccg gcgaactcat ggcgtagtta
    10861 tgagccgccg ggatcgcgca caggtagcgt gtctgttgtg tgaactgaca aatctcgacg
    10921 ctacgacgca cgctgtagta gtagtcgtta tgagtgcgcg ggatcagctt cggtgtaccg
    10981 gtggtgccgc cggaaagctg gaaatagacc acttcatcag caggtgacgg cgtagcagta
    11041 aaaccgtctg ccggatggtt aatcgcatcc tgcaagttat gctcaccgct gtcgttgagt
    11101 agctgcacca cgcgaatgga agaatgctct gcaacaaatg tgttgaggaa atcatccccg
    11161 ctaaacagcg catgttggcg atcggcaatc agcaatgcgg gttcaatctg gctggcatag
    11221 gcgttcagtt cactacgctg atggctgaac aacgccagca ccggcgcaac gcccagtttc
    11281 agcagcgcga aaaaggtaat gtaaagttca gcgacgttac ccagttgtac cagcgcggtt
    11341 tcaccaggtt taatgccctg acggcgtaaa ctacacgcga ggttatccgc cgcctgattc
    11401 agctcccggt aactcaactg tcgctcgccg tcgataaccg cgatgctgtc actcgcagcg
    11461 tggcgagtca gaatgtcagt cagtggcaaa tcctgccagt agcctttttc ccgatagcga
    11521 cgggcaaact cttccggcca gcgggtgaat ggaatgctca tcctcgctcc ttaatgcaat
    11581 ccaaaaacgt tcaacatggt agaaagtttg acgcctgttt cgcgccactc acccaacggt
    11641 gacgacgcag gcacaatccc cgctccggca aacagacgca cctgattttc ccgcagcttc
    11701 gcgcagcgga tggtcaccac ccattcgccg ttaccttcgc tgtcacacca acccacaatg
    11761 ccgccaaaca gttcgcggtc gaacggctcc agttcagcaa taacctgggt cgcggcctga
    11821 tgcgggaaac cgctcagcgc gggggtcgga tgcagcagac aggccagagt cagtgcgttt
    11881 tcttgcgaat tcgctttacc ttcaaaggga gttgcgagat gccacagcgt cggcgtggta
    11941 atcaattgtg gagaggaagg aacgtgtaac tcactactgc gttcgcgcag tacctctttc
    12001 atcgcctgag tcaccagttc atgttcatgg cgatcttttt ctgacgccag cagacgatta
    12061 cccgcttcgc gatcgagcac ttcatccggc tgacgacgtg cggaaccggc taacggaatg
    12121 gagctaaaac gctcaccgtc tttacgtagc agcagttccg ggctggcccc cagcagaacg
    12181 ccaccatctg ccagcgggac atggaagttg taactaaccg ggttttgcgc aatcaaccgt
    12241 tccagcaata cgccactatc aatggcggcg tcagtggtga tatcaatcaa ccgtgacaac
    12301 accactttgt cgacctgcgg cgtggcggta agtgcggcgg cgcgggcaac catctgttca
    12361 aacgtggttt gctccggaat tgcctggcgt tccaccacgt tcagcgactg gctgcgggtg
    12421 aaacggcgtg cggaagcttg tttttcctga cgggagaacg actgccagga ttcaggaata
    12481 tacagcgacg aaggctgacg tggatcgaaa ggaatcgccc cgaccatcac cggatttttg
    12541 atgccctgcg ctttggcatc ggcaaacagc gcggcgagtt tttgctggaa gggactgtcg
    12601 ggcgaatccc cgttcacagc cggttcatcg aagcgggcga aacatcctga cgtcgtaaaa
    12661 ctgcggtacg gcgacataaa gaaaaagcga ttgggcgcaa gtgttgccat ggtctgctgt
    12721 acttcctcag ccagtgacgt atccatatca tcctccacaa aatgataaag gctttaataa
    12781 tgattatcat ttatattttc ggtcgctaac ctaaaagcaa acgcgcacta tgtcaactct
    12841 tgaggtaacg caatctgcaa ctcggggttg catctggttg tggttataat gaaaatgaga
    12901 agcattaact tcattaataa caggaggctg atttgtgaga ctcgccccgc tctaccgcaa
    12961 tgcccttcta ttaacaggac ttttgttttc aggaatagcc gcagttcagg ccgccgactg
    13021 gccgcgtcag attactgaca gccgtggcac tcataccctg gaaagccagc cgcagcgtat
    13081 tgtttccacc agcgtcaccc tgaccggctc actgctggcg attgatgctc cggtgatcgc
    13141 cagcggcgcg accacgccga ataaccgcgt cgcggatgac cagggctttt tacgccagtg
    13201 gagcaaggtg gcgaaagaac gcaaactgca acggctctat atcggcgaac cgagcaccga
    13261 agccgttgcc gcgcaaatgc cggatctgat tttaattagc gcaaccggcg gggattcggc
    13321 gctggcgctg tatgatcagc tttccaccat cgccccgaca ttaatcatca attacgacga
    13381 caaaagctgg caggcgctgt taacgcaact tggcgaaatt accgggcatg agaaacaagc
    13441 ggcagagcgg attgcgcagt ttgataagca actggcggcg gcgaaagagc aaatcaaatt
    13501 accgccgcag ccggtcactg ccattgtcta tactgccgct gcacacagtg ccaatctctg
    13561 gacgccggaa tcagcacaag ggcagatgct ggaacaactc ggctttacgc tggcgaagtt
    13621 acccgcaggg ttaaatgcca gccaaagcca gggtaaacgc catgacatca ttcagcttgg
    13681 tggggaaaat ctggctgcag ggttaaatgg cgagtcacta ttcctgttcg ccggtgatca
    13741 gaaagatgcc gatgctattt atgctaatcc gctgctcgcg cacctgcctg cagtacaaaa
    13801 caagcaggtt tatgcgctgg gaaccgagac gttccgtctg gattactaca gcgccatgca
    13861 agtgctggaa cgacttaagg cgctgtttta agcattaacc gtcagacgct gtcacctgcg
    13921 gcggcgtctg gcgaaaacgt cgcaactcca ccagcaccag caataacaac acgccgataa
    13981 tcaataaacc aaaaccgctc gcgcttgcgg aagcaaccgg tgtcatcatc gctcccaaac
    14041 cacccagcag cgccgcgcct atggcatcgc ccgtcacgtt ttgtgccgtc cacaaaccgt
    14101 taatccgccc taacatcgct tccggggttt gcgtttgcag cattgtgtat tgcagcaacg
    14161 agctgaccgc actcaaccag ccgaacagcg ccagacaaac cacgcctaaa atccacatcg
    14221 gcatcaggcc aaacagacca atggcgagga acgatcccag cgtggagagc agcatcaata
    14281 accctggtcg cgcgctatgc gccagcttcc cgctggttaa cgcgccaata gccgcgccga
    14341 gcgggatcgc cgcatagaga aaaccaatct gtgccgccga catctgccag ttgtcagcca
    14401 gcgccggata cagtacccgc accgcactcg ccatcgtcaa taaaccaccc agcagcgcaa
    14461 tccctcccac cagcgggctg gcgagtagaa aacgaaatcc tgccagtaat gatttcaacg
    14521 gatgctcacg cggctgcggt ggcggtggca gtgccggaag gcttaacaac ggtagcaagg
    14581 taataaacgt gcccgccgcc gccagcccgt agttccaggc cacaccgcca gtcgccagca
    14641 gtaaaccgcc aatcatgggc gaaatcaccg accccagacg cacggtcaac atggtgatcg
    14701 ccccggcctg cattaagttt tcacgcccta ccagtgctgg tgtcgccgcc aatagcgccg
    14761 taacgccaag cgatgcgaaa aaaccatccc ataaaccaag taaatagatt gccagcaatg
    14821 acggctccgg cagcagcgcg ttcaggcaca gtccaatgaa gccaatgcca caggtgccgc
    14881 gcgccagcaa aatcactttt ttgcgctcat agcgatccgc cagcacaccg ccgaccatca
    14941 ggccaacaaa catcgcgccg ccggtcagcg tcaccgaaag ccccacctgc caggtggaat
    15001 gtgtcatcat ctggatctgc accggcaccg cgacgccgag caaacccaga gacacaattg
    15061 agatgaaacg agcgaggaat actgcgcgaa acgccgggtg cgttttcaac aggctgaggt
    15121 taagcagcca ggattgttta ttcattacaa tgccttgcca tcaaatattt taaataccta
    15181 ttcttaggct gcacatgcta acatatccaa ataagatcga taacgataat taatttcatt
    15241 atcatggaag ttcgtatgtc tggttctgtt gccgtgacac gcgccattgc cgtgcccgga
    15301 ttgctgttat tactgattat cgcgacggca ttaagcctgc tcattggggc aaaatcactc
    15361 cccgcttccg tagtgctgga ggccttctcc ggcacctgcc agagcgccga ctgcaccatc
    15421 gtgctcgacg cgcggctgcc gcgtaccctt gccggtttac tggcaggcgg cgcgcttggc
    15481 cttgccgggg cgttaatgca aaccctcacc cgaaacccac ttgccgaccc cggcttgctt
    15541 ggcgtgaacg ccggagccag ctttgccatt gtgctgggtg cggcgctgtt tggttactct
    15601 tccgcacagg aacaactggc gatggccttc gccggggcgc tggtggcctc gttgattgtt
    15661 gcctttaccg gcagccaggg cggtgggcag ttaagtccgg tgcgtttaac cctggcgggc
    15721 gtggcgctgg cggcggtgct ggaagggctg accagcggca tcgccctgct taatcctgac
    15781 gtctacgatc aactgcgttt ctggcaagcc ggttcgctgg atattcgcaa tctacatacc
    15841 ttaaaagtgg tgctgatccc agtgctgatc gccggagcaa ctgcgctatt actgagtcgc
    15901 gcgctgaaca gtttaagcct tggcagcgac accgcgacgg cgctgggcag tcgcgtggcg
    15961 cgcacacagt tgattggtct gctggcgatt accgtgcttt gtggtagtgc gacggcggta
    16021 gttggcccta ttgcctttat tggcctgatg atgccgcata tggcgcgttg gctggtgggt
    16081 gccgatcatc gctggtcgct gcccgtcacg ctacttgcta cccctgccct gctgctgttt
    16141 gccgatatca tcgggcgggt gattgtgccc ggtgaactgc gcgtttctgt ggtcagtgcg
    16201 tttattggtg caccggtgct gatcttcctc gtgcgacgta aaacgcgagg tggtgcatga
    16261 tttacgtctc tcgccgatta ctcatcacct gtttgctgct gatttccgcc tgtgtggttg
    16321 caggtatctg gggattacgc agcggtgccg tcacgctgga aatctcgcag gtattcgccg
    16381 cgctgatggg cgatgcgccg cgcagtatga cgatggtggt caccgaatgg cgtttaccac
    16441 gcgtgctgat ggcgctgttg attggcgcgg cactgggcgt cagcggtgcg atttttcagt
    16501 cgctgatgcg taacccactc ggcagccctg acgtaatggg ctttaacacc ggggcgtgga
    16561 gcggcgtgtt ggtggcgatg gtgctgtttg gtcaggatct gacggctatc gcgctggcag
    16621 caatggtggg cggcattatc acttcgctgt tggtctggct gctcgcctgg cgtaacggta
    16681 tcgacacctt tcggttgatt attatcggta tcggcgttcg cgccatgctg gtggccttta
    16741 atacctggct gttgctgaaa gcgtctttag aaacggcgct aacagcaggt ttgtggaatg
    16801 ccggatcgct caacggcctg acgtgggcaa aaacctcgcc ttccgcaccc atcattatat
    16861 tgatgctaat tgccgccgcc ttactggtac gccggatgcg cttgctggaa atgggcgatg
    16921 ataccgcgtg tgcgctgggc gtcagcgtcg aacgttcgcg tctgttaatg atgctggttg
    16981 cagtggtgct taccgctgcg gcaacagcgc ttgccgggcc gatttccttt attgctttag
    17041 tcgcgccgca cattgcccga cgcattagcg gcaccgctcg ctgggggcta acccaggcgg
    17101 cgctatgcgg ggcgctgtta ctgctggcgg ccgatctctg cgcccaacaa ctgtttatgc
    17161 cgtatcaact tccggttggc gtcgttaccg tcagcctcgg cggtatttac cttatcgttt
    17221 tgttaattca ggagtctcgc aaaaaatgac cgaatcagta gcccgtttgc gcggcgaaca
    17281 gttaaccctg ggatatggca aatataccgt tgcggaaaat ctgactgtag aaatacctga
    17341 tggtcacttc acggcaatta tcgggccaaa tggctgcggt aaatccacgt tgctgcgtac
    17401 cttaagccgc ctgatgacgc ctgctcatgg gcatgtctgg ctggatggcg agcacattca
    17461 acattacgcc agtaaagaag ttgcccggcg gattggtctg ttggcgcaaa atgccaccac
    17521 gctgggagat atcaccgtgc aggagctggt ggcgcgtggg cgttatccac atcaaccgct
    17581 gtttacccgt tggcgcaaag aagatgaaga ggcggtaacg aaagcgatgc aggccacggg
    17641 aataactcat cttgcagatc aaagcgtgga taccctttcc ggtgggcaac gccagcgagc
    17701 gtggatcgcg atggtgctgg cccaggaaac ggcaattatg ctgctcgacg aacccacgac
    17761 ctggctggat atcagtcatc agattgattt gctggaactg ttaagagaac tgaaccgcga
    17821 gaaaggctat accctggcgg cggtgctgca cgatcttaat caggcctgtc gttacgccag
    17881 ccatttgatt gcattgcgag aagggaaaat tgttgctcag ggagcgccga aggagattgt
    17941 cactgctgaa ctgattgagc gcatttatgg tctccgctgc atgatcattg acgatccggt
    18001 agccggaacg ccgctcgtgg tgccgctcgg acgaacggca ccttcaaccg caaatagtta
    18061 aactaagtgg tctgccatca tggcatcctg ttttctggat gccatcgcat agcgcaataa
    18121 cacgctacca caagccacca tcccgccgat cagcgcggaa aggatcacaa taatcgcctt
    18181 acccggaccg tcttttttca ctggcaatga cgggcttaac tgatatttaa acggcgtaaa
    18241 attcacatcg ttgatatttg cttttgttaa ctgctcaaca agatactgac gattacgtaa
    18301 ttcaccgttc agttccgcaa cgtcagtgac cgctttttca atttccagtt tgcgttcaat
    18361 accgtctgcg ccgagagaaa tagagaaatc ggggtcatct ttaacggcct gaccattact
    18421 gtaaacgggc tttttaattc ctgccgcgtt ggcaatgtcg agtgaataat tgaggcgctg
    18481 aatgtttgca tcaagttgat ttttcatttt aatgcggtcc tgagccagtt tttctttttc
    18541 aaactgggtt ttgatctcca gtttattacg gacgttttct atcgactctt tcaccaccaa
    18601 cgcagagata taatcgatat acccgctcaa aacggtctgc gcctcttcac tggttggcgc
    18661 ggtaaaactt agcgtccagg aggtatacag tgacggttca tcttttttct tactggcatt
    18721 gtcatcaacc gctttcattt tttcgctcaa tgcgacaatt gcgcgatgca aatccagttc
    18781 gtcgattttc gcctctttta attggtccat cacataaggt gatgaacgca ggtactcttc
    18841 cagcaagcta accgactgaa acttcttgat aaacaggtta aatgcttctg tacgatcaat
    18901 tttgatatcc agatccagca cacgaagctt agtgaatgtt ttctccaact cttgccactg
    18961 aacaggttct ggaggcgtga caaccgccgc gctggtccat ttttgcggca ggatgaaaga
    19021 gatcagcaag cctgcacagg caaacgcaaa aacgaccgcc atgaccgttt ttttggcccg
    19081 ccataaaact gagattagat taagtaaatc aatttcatta ttactgggcg acgccagagg
    19141 ataatcggga aaatgagcgt cacttccctg tttaatattc agtgatgaca tggggtataa
    19201 cctgaaaatg agtccaatag ggggaaatct ccacggcgct cactttacca gttggtgaga
    19261 attatccgaa gctgaagttt gtaaattcat tccactgaat gcaacattca ctctatttaa
    19321 gaaaaataac cagcaaactt ataaattaaa taaatgataa acccataatt atttcctgca
    19381 attatgggtt tatagataat gttaatttat ctgtttagcg ttgcgcgaat aatcggccca
    19441 attttttcaa atgcccctgg agagataata tccacatgcg cacaatcctg acgatagata
    19501 tccagctccg caatccacgg cgaccaggcg cgttcggggc tcataccttc ctgaagcgta
    19561 cgttcagcaa caaacagcgt cgcttttccg tcaaacggta cgctatgagc agtcgtcagc
    19621 aggcgcacag catcagcgta gttgccttca atggtggtaa acaactccgt tgaagtactt
    19681 ccctgctgtg ccgccaggaa ggcctcgcgc tcgcggttaa tctccgccag cacttccggg
    19741 tccagaccat tagcttcttt ttcctgccag ttttgcgttt ctggcggcca ggtatccagc
    19801 aagccaagaa atgccacctg ttcgccacgg gcacgtagtc gcgccgcaat gccctgcgcc
    19861 agtgtaccgc caagggaata ccccagcagg taataagggc cgtgcggttg ttgttcaagt
    19921 aacgttgcca gatgcgcttc gcagacttca tccaggttcg tcgctgtctg catggggcca
    19981 tgagggcgcg gcgactgaat gccgataatc gaccatagtg gatcgagata acgcgagagc
    20041 acgctaaact gccaggcaaa accggatgcc ggatggaaac aaaacagcgt cgggccatta
    20101 ccttcacgca acggcagaat ggtttcgaat cccatgcgcc gggagctgtc ctcttcacca
    20161 tcaataatcg ttgccagttt ggcgacggtt gacgcgacca tcacctgccc cggcgtcacc
    20221 tgacgggcaa actgccgact taactgcgct gccagtttca ttgccagtag cgaatgaccg
    20281 ccaagcgcga agaaatcagc atcggcatcc tgcacgtcac aacccagcaa cgacgcgaat
    20341 gccgcagcga taatcgtttc actgcccgct ttcggcgcac gccccggcgt ttgtgcctta
    20401 agttcaggca acggtaaggc tttgcgatcc agcttgccgt tggcgctaag aggtaactgt
    20461 ggaagttgca gcagaacgac cggcgccata tgcggcggca atgtttcgcg aagctgtgcc
    20521 tgtaatgcgc tggtatccaa cggcagacct gattgcgaca ccagatagcc caccaactga
    20581 cgcgcatcac caccggtggc tgccgcctgg ttaatcacac aggcgtgggt aacggcttgt
    20641 tcgacatccg gcagcgcctg catcacgcga tcgatttcgc ccagttcgat acgctgcccg
    20701 cgaattttta gctgatcgtc actgcgcccg aggtactcca ccgcgccgtt atccagccag
    20761 cgggcaacgt ctccggtacg gtacatccgt tcaccaggga caaaaggatc ggcaatgaag
    20821 cggctggcgg tcagatcggg tcgtccaaga tacccctgcg ccagttgaat accggtgaga
    20881 tagagatctc ccgccacacc cggcggcacc ggatgcatca tcgcatcgag aatgcgcaag
    20941 cccgtattcc acaccggata accaatcggc acactgctgc cgcgcacctg tgccagttcc
    21001 tcgccaaaag ccggatacca actcacatct accgccgctt ccgtcgggcc atatagatta
    21061 tgcaacggcg cgcccgttaa ctgttgccat tcgcggcata aatcagccgg taaggcctca
    21121 ccactacaga aaacctgttt caacgtcgcg caattctggc gagcggtttg cggcgtcagc
    21181 gatgcaacaa atgccgccag catcgacggc acaaagtgcg tggtcgttac gccatattcg
    21241 gcaaagaatt gctgcatagc gagcgggtcg cggtgcgctt ccggttcagc catcaccagt
    21301 ttagccccgg caataaacgg ccagaaaaac tcccacaccg agacatcaaa actgcacggc
    21361 gttttttggg caacgacatc ttcacctgta agtggataat gattttgcat ccacaacagg
    21421 cggttaacga tagccgtctg cccgaccatt acccctttcg gcctgccggt ggagccagag
    21481 gtaaagatga tataagccgt gtgatgcggt tgtgaaagtt gcagcggcgc actgccctgc
    21541 ggtgtaagcg gggcgttata gcaaaggttg gttaaatctg gaacatcggc aaagcgcggc
    21601 agttgatcgt cggtggtgat taacagcgac ggacgcgcat cttccagcat cattttcagg
    21661 cgatcgtccg gataaccggt atccagcggt aaccaggccg cacctgcttc aacaatcgca
    21721 tgtagcgcca gggtcaaaaa gaccgagcgc ggtaatgcca ccgccacgct gtcccctggt
    21781 ttaacgccgt gctcacgcag cagattcgcc agcgccacca cctgctcgcg catttcccga
    21841 tagctgaact ggtaacgcgc atctgccagc gccggagcat ccggtgtttt tgccgcttgt
    21901 tctgccacca gcgcgctaag cgtggtttct ggaatctcaa cctgagtggc gttgatctgc
    21961 gccagctgcg catactcacc tggcagcata atatcaacat cgccgcacaa cagagccgga
    22021 tccgcagcga attgggcgat cagcattttc aggcgttcag catgctggat taacgttggc
    22081 tcatcgtaat gctgtttatt ggcgaggatc tcaatactca aatcaccgtg ttcatccggg
    22141 aacagggcca gttcaaggtc attaaccgga ccggttgcca gggtatgggt ttgcgcctga
    22201 acaccaggaa tatccagttg gtaatcaaat accttgatat tgagtaccgg accaaacagc
    22261 ggttcatcac ctgccgctcg cccgctgtca cggacaattt gttcggcatc gtaacgttga
    22321 tgacgacgca tttttttcag ttgtgctgcc agtcgggttg ccagttccgg cagcgtttct
    22381 tgtgccgcaa tgtgaatacc caacggtaaa acgttgagca cgggtccggt agccgtcagc
    22441 gccgccgagc ccagtcgacg cataaagata aatccggcgg cgtagtccat gcgattgcac
    22501 aatcgcccca gccaaaaggc tgccagcgca agggctaaat cggtacgctg cacacctgaa
    22561 agttgcgtag ccagctggcg gaattccccg tcggtaaatt ccagtttcag gcgtagaata
    22621 tctgccgaag cgctgcgccc cgctaaaggt gccggagaaa gtgacgcggg cggcggcagt
    22681 tgacgacgct gttccgccca gaatgccgca tcacgctgcc aggcttcgct ttcgcggtat
    22741 tgctggtact cttccactac atcagcgaaa ggcgtaaacg gcgaagcagg cgtttgttcg
    22801 ccacgcagca atgcgcagta aatgttggcg atctgccgag taatggccgg gaaactgaag
    22861 ccatcgacca gcaaatggtg ataacgctga taccagtacc agcggttatc tgccacctgt
    22921 atcagctggt gaaagaccag tggtttaccg ctatcgacgc gcaaatcttg ttgcaaatcc
    22981 gcctgcatta atgcctgcgc agtaccgtgc ggatcaatat tggttcgcag gtcgataatt
    23041 tctggcagtt cgaatatcag cgcatcatcg acccattgcc agacttcgcc gttatcttcc
    23101 gtaaaacgca tccgcagcgt atctgcttgc gctaatccgg caaccaccgc gcgggccagt
    23161 aatggcgcat caacctctcc ggttaactca acgtaatgcg ccacgctcca ggcggagggt
    23221 aattctgaca gtttttctgc catccagatg ccgggctgtg cggcgaccaa aggtaaatgc
    23281 tggctcattg tgcctcctgc aactgggtga aattcgccgg tgtcagagta cgccagtgga
    23341 cttccagcca ctgctggcag gactcttgtg actgcggctg acacacaatg tcccagcctg
    23401 ccggtaaggc gcattgttgc ggccacagac tgaattgccc ctgcgcattg cgcaaaatgt
    23461 aaaacactcc ctgtggatca tcgaagggat tactgaatgc catattcaac tcctgtcatg
    23521 gaaaagtggt tgccagaggt cgattaaccc ctgcatcaag ccaccgcgcc aacaaagcgc
    23581 atcatgtccg ccgtcaacct gacgccagaa aatggattct tttatagggt gtaattgcgc
    23641 atacagcgcc tgattggccc gcatgatcat cggctcgcga atacccgctt ccagcacaat
    23701 gcgcagacct tcggcgctaa cttcaccagc ttttagcttt tcaagtaaca cgccctcttg
    23761 ctgcccgccc cgatgcggcc accagtacga tcctgactgg cttaatacac agccaaagcg
    23821 ttcaggccag tgcagtccgg catacagcgc ggacagccca ccaaaactct gcccggcgac
    23881 cacggtgcga tcggcgcgat cgctaaaagg ggcaatagct ttcaccaggg gtaataactc
    23941 ttgctgtact gccagccaga aatccgcatt acacggcagt tcgtgggcgc ggtgcgtggt
    24001 gtcgatagcg tcgatcaaca catacacggc gggaggaagt tgctgacgat gggtcagcga
    24061 aatcagcaca ggccagacgg gcatactttg cgcccaaaat tcgccatcga gcaaaactgc
    24121 cagcgggcgt tcttctgctg ttacatcgcc ggtggtaaaa atccatacac gacgtgaatt
    24181 tttcaaccgt tcacttttcc agataatttc tttggctggt atttctggcg cttgcggaca
    24241 atcccatccc ggttgcagag gcgcttgcgg catttcgagt gcagaaacag cgtgccctcg
    24301 cccgcctttc cagctttgtg gattcagcgg atcggctatt gcctggggta atagttttcg
    24361 ccagccttca cgtaattcga ggcgatcggg ggatggtgca gaaaaaatgt catcgcgttc
    24421 ggtgggaata aagcagtagc tgccgcgcca gttggcattg agttgtgtcg tccactgcca
    24481 gacatcagta cctgcaattc gctgcatcga ctggggctgg ctgttctgat gatgatcggt
    24541 cacaccagtg atgtagatcc atacgcgctt tatcgtcgag tattcttcag aaccttgggg
    24601 atcacgccac cagaaagtga cctcaaacat ttcgtcattc agacgctgcc attccgggcc
    24661 atgtttcgac tgccaccagc tctcacttcc tacttttaac gccgtcacac cataacccca
    24721 tgtttactgt gcaatttttc attgattgca gaaatatatt gataatatta ttgataacta
    24781 tttgcatttg caatagcgta atggcgcgcc gtgggaagcg cggacattaa ttaaccaact
    24841 gcactgcgtg tctttcagga tcaaaggttt tcgcggtagc gggatgcgtc gtgttgatga
    24901 cgaccatgcc cgacagttgc aattcgtggc aaaaatgcag gaataaaaca atgaacaaga
    24961 agattcattc cctggccttg ttggtcaatc tggggattta tggggtagcg caggcacaag
    25021 agccgaccga tactcctgtt tcacatgacg atactattgt cgttaccgcc gccgagcaga
    25081 acttacaggc gcctggcgtt tcgaccatca ccgcagatga aatccgcaaa aaccccgttg
    25141 cccgcgatgt ttcggagatc atccgtacca tgccaggcgt taacctgacc ggtaactcca
    25201 ccagtggtca gcgagggaat aaccgacaga ttgatattcg cggtatgggt ccggaaaaca
    25261 cgctgatttt gattgacggc aagccggtaa gcagccgtaa ctcggtgcgt cagggctggc
    25321 gtggcgagcg cgatacccgt ggtgatacct cctgggtgcc acctgaaatg attgaacgta
    25381 ttgaagttct gcgtggtccg gcagctgcgc gttatggcaa cggcgcggcg ggcggcgtgg
    25441 ttaacatcat taccaaaaaa ggcagcggcg agtggcacgg ctcctgggac gcatatttca
    25501 atgcgccaga acataaagag gaaggtgcca ccaaacgcac taactttagc ctgaccggtc
    25561 cgctgggcga cgaattcagc ttccgcttgt atggcaacct cgacaaaacc caggctgacg
    25621 cgtgggatat caaccagggt catcagtccg cgcgtgccgg aacgtatgcc acgacgttac
    25681 cagccgggcg cgaaggggtg atcaataaag atattaatgg cgtggtgcgc tgggacttcg
    25741 caccattgca atcgctggaa ctggaagcgg gttacagccg ccagggtaac ctgtatgcgg
    25801 gcgataccca gaacaccaac tctgacgctt acactcgctc gaaatatggc gatgaaacca
    25861 accgcctgta tcgccagaac tactcgctga cctggaacgg tggctgggat aacggcgtga
    25921 ccaccagcaa ctgggtgcag tacgaacaca cccgtaactc gcgtattccg gaaggtctgg
    25981 cgggcggtac cgaagggaaa tttaacgaaa aagcgacaca ggatttcgtc gataacgatc
    26041 ttgatgacgt gatgctgcat agcgaagtta acctgccgat tgatttcctc gttaaccaga
    26101 cgctgacgct gggtacggag tggaatcagc agcggatgaa ggacttaagt tccaacaccc
    26161 aggcactgac cggaacgaat accggtggcg ctattgatgg cgtgagtgcc accgaccgta
    26221 gcccgtattc aaaagcagaa attttctcgc tgtttgccga aaacaacatg gagctgactg
    26281 acagcaccat cgtaacgccg gggctgcgtt tcgatcatca cagtattgtc ggcaataact
    26341 ggagcccggc gctgaacata tcgcaaggtt taggcgatga cttcacgctg aaaatgggca
    26401 ttgcccgcgc ctataaagcg ccgagcctgt accagactaa cccgaactac attctctaca
    26461 gtaaaggtca gggctgctat gccagcgcgg gcggctgcta tctgcaaggt aacgatgacc
    26521 tgaaagcaga aaccagcatc aacaaagaga ttggtctgga gttcaaacgc gacggctggc
    26581 tggcgggcat cacctggttc cgtaacgatt atcgcaataa gattgaagca ggctatgttg
    26641 ctgtagggca aaacgcagtc ggcaccgatc tctatcagtg ggataacgtg ccgaaagcgg
    26701 tggttgaagg tctggaagga tcgttaaacg taccggttag cgaaacggtg atgtggacta
    26761 ataacatcac ttatatgctg aagagtgaaa acaaaaccac gggcgaccgt ttgtcgatca
    26821 tcccggagta tacgttgaac tcaacgctga gctggcaggc acgagaagat ttgtcgatgc
    26881 aaacgacctt cacctggtac ggtaagcagc agccgaagaa gtacaactat aaaggtcagc
    26941 cagcggttgg accggaaacc aaagaaatta gtccttacag cattgttggg ctgagcgcga
    27001 cgtgggatgt gacgaagaat gtcagtctga ccggcggcgt ggacaatctg ttcgacaaac
    27061 gtttgtggcg tgcgggtaat gcccagacca cgggcgattt ggcaggggcc aactatatcg
    27121 ccggtgccgg ggcgtatacc tataacgagc cgggacgtac gtggtatatg agcgtaaaca
    27181 ctcacttctg atgctaacgt cagattgttg acaaagtgcg cgtcgttcat gccggatgcg
    27241 gcgtgaacgc cttatccggc ctacaaaaat cttgccaatt caatatattg caggaccatg
    27301 taggcctgat aagcgtagcg catcaggcag ttttgcgttt gtcatcagtc tcgaatatgg
    27361 tcgatatgaa aactacgcat acctccctcc cctttgccgg acatacgctg cattttgttg
    27421 agttcgatcc ggcgaatttt tgtgagcagg atttactctg gctgccgcac tacgcacaac
    27481 tgcaacacgc tggacgtaaa cgtaaaacag agcatttagc cggacggatc gctgctgttt
    27541 atgccttgcg ggaatatggc tataaatgtg tgcccgcaat cggcgagcta cgccaaccgg
    27601 tgtggcctgc ggaggtatac ggcagtatta gccactgcgg ggctacggca ttggccgtgg
    27661 tttcccgtca accgattggc gttgatatcg aagaaatttt ttctgcgcaa acagcgaccg
    27721 aattgacaga caacattatt acaccagcag aacacgagcg actcgcagac tgcggtttag
    27781 ccttttctct ggcgctgaca ctggcatttt ccgccaaaga gagcgcattt aaggcaagtg
    27841 agatccaaac tgatgcaggt tttctggact atcagataat tagctggaat aaacagcagg
    27901 tcatcattca tcgtgagaat gagatgtttg ctgtgcactg gcagataaaa gaaaaaatag
    27961 tcataactct gtgccaacac gattaattga caacatctgg tacgattcgc ccgcagccat
    28021 cactgactgc gggcgaaagt gtaaagcagg tgccttacca ctctgacctg acaaccggat
    28081 atgcggggat tgctccccgc acaacggcta cttcttcggt tcgtaagcga gaacagcctt
    28141 aaactcaata ttacgttcct ttacggtgaa ctcacacagc gagtctccga ccagaagcgt
    28201 aaatcccagc accgtcaaac acagcactat gactgccaca agggcatatt tcgtcagcat
    28261 gtttatattg cctccatgcg aagaagggac taccatccga gttgttgggg ctcggagtgg
    28321 cagccccagg attgatagaa atatctcctg gggcttcatc tttccagacc tcagagttaa
    28381 cctgaaaccg gaaagcttca ggcacccgcc gttatcttac ccttccttag caaaatagct
    28441 gtttatttat acagtgaatc ctgtttcgga cttctgtcgg caaaactcat cattgctctt
    28501 aaaacgaaat cgcccgcagc catcactgac cgcgggcgaa agtgtaaagc aggtgcctta
    28561 ccatcctgac ctgacaaccg gatatgcggg gattactccc cgcacaacgg ctacttcttc
    28621 ggttcgtaag cgagaacagc cctgaactca atattacgtt ccttcaccgt aaactcacac
    28681 agcgagtctc cgaccagaag cgtaaatccc ggtaccgtca aacacagcac tatgactgcc
    28741 acaagggcat atttcgtcag catgtttata ttgcctccat gcgaagaagg aactaccatc
    28801 caaattgtct ggtttggagt ggcagcccca gggttgatag aaatatctcc tggagcttca
    28861 tctttccaga cctcagagta aacctgaaac cggaaagctt caggcacccg ccgttatctt
    28921 acccttcctc aacaaaatag ctgtttattt atacagcaaa tcctgtttcg gacttctcac
    28981 tcctcattta atcaactgaa gctgagcccg ttttttctgc atgcctcaat cgtcaaaatt
    29041 cttcgccacc agccagccgt gccataactt gtcacattgc aaaagtagcc aaattgcttc
    29101 agtcaccatc accaaaaaaa gaaacattcc agaacttccg catctcgccc agccgctccg
    29161 acttctatac tgaatagaaa actccaacat aagagaaacc tatgccatta cccgattttc
    29221 atgtttctga accttttacc ctcggtattg aactggaaat gcaggtggtt aatccgccgg
    29281 gctatgactt aagccaggac tcttcaatgc tgattgacgc ggttaaaaat aagatcacgg
    29341 ccggagaggt aaagcacgat atcaccgaaa gtatgctgga gctggcgacg gatgtttgcc
    29401 gtgatatcaa ccaggctgcc gggcaatttt cagcgatgca gaaagtcgta ttgcaggcag
    29461 ccgcagacca tcatctggaa atttgcggcg gtggcacgca cccgtttcag aaatggcagc
    29521 gtcaggaggt atgcgacaac gaacgctatc aacgaacgct ggaaaacttt ggctatctca
    29581 tccagcaggc gaccgttttt ggtcagcatg tccatgttgg ctgtgccagt ggcgatgacg
    29641 ccatttattt gctgcacggc ttgtcacggt ttgtgccgca ctttatcgcc ctttccgccg
    29701 cgtcgccata tatgcaggga acggatacgc gttttgcctc ctcacgaccg aatatttttt
    29761 ccgcctttcc tgataatggc ccgatgccgt gggtcagtaa ctggcaacaa tttgaagccc
    29821 tgtttcgctg tctgagttac accacgatga tcgacagcat taaagatctg cactgggata
    29881 ttcgccccag tcctcatttt ggcacggtgg aggttcgggt gatggatacc ccgttaaccc
    29941 ttagccacgc ggtaaatatg gcgggattaa ttcaggccac cgcccactgg ttactgacag
    30001 aacgcccgtt caaacataag gagaaagatt acctgctgta taaattcaac cgtttccagg
    30061 cctgccgcta tgggctggaa ggcgtcatta ccgatccgta cactggcgat cgtcgaccac
    30121 taacggaaga caccttgcga ttgctggaaa aaatcgcccc ttctgcacat aaaattggtg
    30181 catcgagcgc gattgaggcc ctgcatcgcc aggtcgtcag cggtctgaat gaagcgcagc
    30241 tgatgcgcga tttcgtcgcc gatggcggct cgctgattgg gctggtgaaa aagcattgtg
    30301 agatctgggc cggtgactaa gccgggaatt gccttacaga agaatatccc tgacaatggt
    30361 gttttaactt acatttaaca atgttatgaa acacccttta gaaaccttga ccaccgcagc
    30421 aggcattttg ctgatggctt tcctctcttg cctgctgctg cccgcccccg cactggggct
    30481 tacgctggca caaaaactgg tgaccacgtt ccatctgatg gatcttagtc agctttacac
    30541 tttattgttt tgtctgtggt ttttagtgct gggcgctatt gagtattttg ttctgcgctt
    30601 tatctggcga cgctggttct cgctggcgga ttaaacgtcg attatcgccg tacttcgcgg
    30661 catactttgc ttattctcct tcgcctaaag gaacgtttat ggataagcaa tcactgcacg
    30721 aaacggcgaa acgcctggcc cttgagttac cctttgtcga gctttgctgg ccttttggcc
    30781 cggagttcga tgtttttaaa attggcggca agatttttat gctgtcgtcg gagctacgcg
    30841 gcgtcccctt tatcaatctg aagtccgatc cacaaaaatc cctgttaaat cagcaaatat
    30901 acccaagcat taagccaggg tatcacatga ataaaaagca ctggatttcg gtgtatcccg
    30961 gcgaggaaat ctccgaagcg ttacttcgcg atctgatcaa cgattcgtgg aatctggtgg
    31021 ttgatggtct ggctaaacgc gatcaaaaaa gagtgcgtcc aggctaaagc ggaaatctat
    31081 agcgcatttt tctcgcttac catttctcgt tgaaccttgt aatctgctgg cacgcaaaat
    31141 tactttcaca tggagtcttt atggatatca tttctgtcgc cttaaagcgt cattccacta
    31201 aggcatttga tgccagcaaa aaacttaccc cggaacaggc cgagcagatc aaaactctcc
    31261 tgcaatacag cccatccagc accaactccc agccgtggca ttttattgtt gccagcacgg
    31321 aagaaggtaa agcgcgtgtt gccaaatccg ctgccggtaa ttatgtgttc aacgaacgta
    31381 aaatacttga tgcctcgcac gtcgtggtgt tctgtgcaaa aaccgcgatg gacgatgcct
    31441 ggctgaagct ggttgttgac caggaagatg ctgatggccg ctttgccacg ccggaagcga
    31501 aagccgcgaa cgataaaggt cgcaagttct tcgccgatat gcaccgtaaa gatctgcatg
    31561 atgatgcaga gtggatggca aaacaggttt atctcaacgt cggtaatttc ctgctcggcg
    31621 tggcggctct gggtctggac gcggtaccca tcgaaggttt tgacgccgcc atcctcgatg
    31681 cagaatttgg tctgaaagag aaaggctaca ccagtctggt ggtagttccg gtgggtcatc
    31741 acagcgttga agattttaac gctacgctgc cgaaatctcg tctgccgcaa aacattacct
    31801 taaccgaagt gtaattctcc cttgccgggc atctgcccgg ctatttcccc tcagattctc
    31861 ctgatttgca taaccctgtt tcagccgtca tcataggctg ctgttgtata aaggagacgt
    31921 tatgcaggat ttaatatccc atgttgaaga tttagcgggt attgagatcg atcacaccac
    31981 ctcgatggtg atgattttcg gtattatttt tctgaccgcc gtcgtggtgc atattatttt
    32041 gcattgggtg gtactgcgga ccttcgaaaa acgtgccatc gccagttcac ggctttggtt
    32101 gcaaatcatt acccagaata aactcttcca ccgtttagct tttaccctgc aggggattat
    32161 cgtcaatatt caggcggtat tctggctgca aaaaggcacc gaagcggcag atattctgac
    32221 tacctgcgcg cagttgtgga tcatgatgta tgcgctgctt tcagtcttct cgctgctgga
    32281 tgttattttg aatctggcgc agaaattccc ggcagcatct cagttaccgc tgaaagggat
    32341 atttcagggg attaaactga tcggcgcgat tctggtcggc attttgatga tctcgctgct
    32401 gattggtcag tcgccagcga ttctgatcag cggtcttggt gcaatggctg ccgtgctgat
    32461 gttggtattt aaagatccga ttcttggtct ggtggcaggt attcagcttt ccgcgaacga
    32521 tatgctgaaa ctgggcgact ggctggagat gccgaaatac ggcgcggatg gcgcggtgat
    32581 cgatattggg ttaaccaccg tcaaggtgcg taactgggac aataccatta ccactattcc
    32641 cacctggtct ctggtttctg actcctttaa aaactggagc gggatgtcag catctggcgg
    32701 gcgacgtatt aagcgcagta tcagtattga tgtcaccagt attcgttttc ttgatgaaga
    32761 cgaaatgcaa cgtctgaata aagcgcattt gttaaagcct tatttaacca gccgccatca
    32821 ggaaattaat gagtggaatc gccagcaagg ttctacggag tcggtattaa acctgcgccg
    32881 aatgaccaat attggaacct ttcgtgccta tctgaacgaa tatctgcgta accatccgcg
    32941 gattcgtaaa gatatgacct taatggtacg ccaactggct ccaggtgata acggtttacc
    33001 gctcgagatc tatgcgttta ccaacaccgt ggtgtggctg gaatatgaaa gcattcaggc
    33061 tgatatattc gatcacatat ttgcgattgt cgaagagttt ggtctgcgac ttcatcagtc
    33121 gccaaccggc aatgatattc gctctctggc gggtgcattt aagcagtaat taaaaaaacc
    33181 gctctcatcg aatggatgag agcggacatc gttttgtcgg atgtagcgtg aatgccttat
    33241 ttccgacgca gcgttttaaa tgctacaaac aggaatacaa tccacaccgg cagcaggatc
    33301 gctgacaagc gcatatcatc catcgtgcac atcagcagca aaatcatggc gaggaaggcg
    33361 atgcaaagat agttgccgaa cggatagagc agcgccttaa actgtgtttc acgcccctga
    33421 cgtcgcatcg ctgcacgaaa acgcagatgc gccagacaga tcataatcca gttcaacagc
    33481 agcgttgcta ccaccagcgc catcagcaga ccaaacgctt tttgcggcag cagatagttg
    33541 attaacacca ccagcgaagt gatcgctccg gaaagcatca gcgagttaat cggcacgccg
    33601 cgacggctaa cacgggtaag gaacttcggc gcattaccct gcacagaaag gccaaacagc
    33661 atgcggctgt tagagtaaac cccgctgtta tacactgaca gcgatgctac cagaatgacg
    33721 aagttcagcg cagaagctac cacgttgctg tcgagattat ggaaaatcat cacaaacggg
    33781 ctactgttgg atttcacttc cacccacgga tagagcgcca gtaaaaccac cagtgaaccg
    33841 atgtaaaaca gcaggatgcg atacaccacc tgatttaccg cttttggaat gcttttttcc
    33901 ggatcgcgcg cttcagcggc agtaatccca atcagctcca gaccgccgaa ggagaacata
    33961 attaccgcca gcgacaaaat cagcccattc cagccggtgg cgaagaaacc accgtagcgc
    34021 cagaggttgt cgatactggc tttctcgccg ccgtgaccag aaaacagcag ccacaggcca
    34081 aagccgatca taccgatgat tgccagcact ttaatcagcg caaaccagaa ctcggtttcg
    34141 ccatataagc gcacgttcac caggttaacg gcgttgatga taataaagaa ggcggcagcc
    34201 caaatccacg ttggaacatc cgggaaccag tactgcatat agatgcccgc agcggtcagc
    34261 tctgccattc ccaccagcac gaacatcacc cagtagttcc agccggagag gaacccggcg
    34321 aacggtcccc agtatttata ggcaaagtgg gcaaatgaac cggataccgg ctcttcgacc
    34381 accatctcgc cgagctggcg cataatcagg aaagcgatga tcccggcgac gccgtagccc
    34441 agcaatacag ccggacccgc catctgaatc gccgggccaa tgccaagaaa cagaccagta
    34501 ccaattgcgc cacccagcgc aatcagttga atatgacggt tatgtaatcc gcgatgaagc
    34561 gtcggctctt gattcgacgc agtatcttcc gatacgtttg acgcgttttt cacgcctttc
    34621 ccctgtgtgt cttttttgtt gaggggcacc ttttaacatt tagtgcccat cgtcgcaaga
    34681 cacaatccac acggttaaac cgggtatcct gcttttattt ccgtacccga tgtcggtgca
    34741 gccacatcag cttatacgct gccgggataa taaacagcga cagcaaaggc gcggtgatca
    34801 tgccgccaat catcggcgcg gcaatccggc tcatcacctc tgatcctgcc cccgttcccc
    34861 acagaatcgg cagcagacca gcgataatca ccgccaccgt catcgctttc gggcgcacgc
    34921 gcaggaccgc gccgtgatgt aacgcctcat ccagcttctg ctcgctgaat gtttgcggat
    34981 tattcaacga cggctcggcc tctatggcgt gacgtaaata catcagcatc accacgccaa
    35041 attcggcggc gaccccggca agggcaataa aaccggtgcc cgtcgccaca gaaagatgaa
    35101 agcccatcca ccacagcaac cagatgccgc ccaccagcgc aaacggtacg ctgctgataa
    35161 tcagcaacgc ttcgcccacc cgacggaacg ccagatacaa cagcacgaag atgatcatca
    35221 acgtcatcgg caccatgagt ttcagcttat ggttggcgcg ctccagtagc tcgaactgcc
    35281 cggagaatgc cacgctggtg cccggtttta actgcacttt ttcagctatc gctttttgca
    35341 aatcgtgaac caccgacacc atgtcacgat cgcgggcatc gatataaatc cagctcgtcg
    35401 ggcgcgcatt ctcggttttc agcatcgacg gtccggtaga gactttaacg tcggccacgt
    35461 ctgccagggt gatttgctgc ttcatcggcg tcaggatcgg caactggcgc agtgcctgcg
    35521 gactatcgcg ccagctttgc ggataacgca gattaattgg ataacgggca atcccttcca
    35581 ccgtttcgcc aaccatcgcc ccgcccaccg cagaagtcac aaacaactgc acatccgcca
    35641 ccgtcatacc gtaacgtgcg gctttttcac ggttaatctc aacgttgata tagcgcccac
    35701 cttccagccg ctcggcaagc gcagaagcca cgcctggcac cgttcgtgcc acctcttcaa
    35761 tttgctccgc catcgcgtcg atatccgcca gcacagtgcc ggaaactttt atgccgatag
    35821 ggcttttaat gccggttgag agcatatcga tacggttacg aattggcggc acccacagat
    35881 tcgccagccc cggcagacgc acggtgttat ccagttcctc aatgattttg tccattgtca
    35941 tgcctggccg ccactgctcc tgcggcttaa gctggatggt cgtttctacc atctccagcg
    36001 gagcagaatc ggtggcggtt tccgctttcc cggttttgcc aaatacccgc gccacttcag
    36061 gtacgctcat aattagcttg tcggtttttt gcagcatact cgccgcctct gctgcggaaa
    36121 tccccggcag cgtcgatggc atatacaaca agtcgccttc attgatctgc ggtaaaaatt
    36181 ccccgccaac tttattgagc ggccagagaa ccgtcagcac cgaaagcgct gccaccaaca
    36241 gcgtggtttt cggccagtgc agtactttca gcaacagcgg atgataaaca cgaatcaaaa
    36301 agcgattgag cgggttactg ctttccggcg gaattttgcc acggatccag taacccatca
    36361 ggatagggat cactacgatc gccagcagcg ccgcacccgc catcgcatac gttttggtga
    36421 acgccagcgg gccaaacaga cgcccttcct gcccttccag ggtgaagatc gggataaacg
    36481 acagcgtgat aatcagcaga ctgataaaca gcgccggccc cacttcaaca gatgcattgg
    36541 taatcacctg ccagcgcgtt ttattatcca gcgtggcgtc aggatgctgg tgctgccact
    36601 cttccagccg tttatgtgca ttctcgatca tgacgatagc ggcatcgacc atcgccccga
    36661 cggcaatcgc aatgccaccc aacgacataa tattggcatt cagcccctgg aagtgcatga
    36721 caataaaagc aatacacaac cccagcggca acgaaataat cgccaccagc gccgagcgca
    36781 catgccagag aaacagcgca cagactaccg ccaccacaat aaactcttcc agcaacttgc
    36841 cgctgaggtt gtcgatggcc cggtcaatga gctggctgcg atcgtatgtt gtaactatct
    36901 ccacgccttc cggcagactg cttttcagcg tttccagttt gtccttcacg gcggcgatca
    36961 cttcacgggc gtttttgccg gagcgtagga tcaccacccc gcccacaact tcgccgttca
    37021 gttcggcaat gccccggcgc atctccgggc caacctggat cttcgcaaca tcacgcagat
    37081 aaacaggcac gccattttca ctggctttta aaacgatgtg attaaagtcg tcgagcgttt
    37141 gtagatagcc gctggcgcgc accatatatt ctgcttccgc cagttcgatt gacgaaccgc
    37201 ccgcttcctg gtttgaagca tccagcgcgc tttttacttc ggcgagactg atgccatact
    37261 gcgccaggcg ctgaggatcg ataaccacct gatactcttt caccacgccg cccaccgacg
    37321 ccacttccgc aacgtcaggg atggttttta gctcatattt gagaaaccag tcctgcaatg
    37381 agcgtaaatc ggccagatcg tgcttaccgc tgcgatccac cagtgcatat tcatagatcc
    37441 agccaacacc cgtggcatct ggtcccagct cggcgctgac tcccgcaggc agcttaccct
    37501 gtacctggtt gaggtactcc agcacccgcg aacgcgccca gtacggatcg gtaccatctt
    37561 cgaaaatgac atacacataa gagtcgccaa actgcgagaa accacgcaca gtcttcgcgc
    37621 caggcaccga caacatggtg gtagttagcg gataagtcac ctgattttca acgatttgcg
    37681 gtgcctgacc gggatagctg gttttaataa tcacctgcac atcggagaga tccggcaacg
    37741 catccactgg cgtgttaatg atggtccagg tgccccagat actcagaaat aacgcgccca
    37801 tcagcaccaa aaaacggttc gccaccgagc gacgaataat ccattcaatc attggttatt
    37861 ccctcaatgc gcatgggtag cactttcaga gcgcatccgc tccagtgcgc cagaaatatt
    37921 ggcttcggaa tcaatcagga acaggccgct ggaaaccacc ttttcacctt ccgccagacc
    37981 agagcgtaat gcggtgacac cttgcgatgc ctggaaaacg gcaacgcgtt ttggtacaaa
    38041 gcgcccgtcg gcatcaacgg taatcacccg ctgttcgctg ccggtatcaa tcagcgcctg
    38101 tgacggaatg agcagcatcg gttcgctggc ggtattgagt tgcaaccagg cgttcattcc
    38161 cggttttagc gcctcgtcgg cgttgtcgac ttccagacgc agctgcagcg tgcgggtcgc
    38221 ggcatccacg ccaggtagta gcgtccattt gcggatggtg agtgttttat caggtcgcgc
    38281 cggaacggtg agcgtaaact gcgaggcatc tttcaccagc caggcgatgg actccgggat
    38341 cgcagcagtg acccacaccg ggtccatacc ctgaattttc gctaccacgt tatctttggc
    38401 gatattcatt cccgcgcgca gatcaaacgc ggtgatcacg ccatcaatgg gtgctttgag
    38461 cgtaaagcga gtctggattt tttgcgtggc gatcaggcgg cgaatatccg cctccggcat
    38521 tcccgccagt cgcagccgct caagaatgcc ttcggtctgg gtcgccgtac cgccggtttc
    38581 gcgcagcagt aaatactcac tctgcgcttc cacccagtcg ggaatggtca ggtcgagaag
    38641 cggcgcgccc ttttgtactt tatcgcccac ggtaagcgga tacaccttgt cgataaaccc
    38701 ggcggcgcga gcctgcacaa tggcatactg atactcgttg taactgacat tcgccgggaa
    38761 actctgggca aaagtcagcg gcccgcgcgt gacggtagcc gttttcaccc ccagattctg
    38821 cgtctgagtc gggtcaatgc gcacaccaga cgcagaactc tcttcatcgg catatttcgg
    38881 caccagatcc atatccataa acggcgattt acctggttta tcgaaccgcg tattgggata
    38941 catcgggtcg taccagaata agattttacg ttctgcggtc gacgtttttt ctgcaggcgg
    39001 ttccgccttt gcaaaccagg taaaacctgc cgcagaaata ataccgcccg cgatcatgct
    39061 gccgataata agcgcgattt ttttcatctc attaaacctg ggttactggc tgactttaat
    39121 atcctgtaat aaagaaaggt tgccctgctg gacaaaatta aacgccactt tgtcgccggt
    39181 tttaattcca ctcattttcg tctgcggggt gatggtaaag cgcatggtca tctccggcca
    39241 gttcacggca gcaatcggat cgtgatggat ggtgattttt ttgctttcca gatcaaaacc
    39301 ttttaccacg ccggtggcac taatgacctg tggttgtgct tcgctcatgg tttcatgatg
    39361 atgttcgtta gcctgggcat taaagccaat aacggtaaac agactgaaca ttgcgacttg
    39421 cagtgctttt ttcatttatt ttctcctgga gttaaaaatt attgctgcca accgccacca
    39481 agtgcggtat ataaagaaat ttcgttgacc tgacgggcat aattcaggtc aagtacggtt
    39541 tgtcgggttg caaataaaga acgctcggca tccagcactt ccagataact tactgcgccg
    39601 tgctgatata atgcccgcgc ccgttgcaaa gtaatttgca gcgatgccag ataacgctgc
    39661 tgggcgctaa tttgatcgtt caggctttga cgtaatgcca gcgcatctgc cacttcttta
    39721 aaggcgttct ggattttctg ttcataattc actaccgact gctgctggcg aatttcggcg
    39781 atatccagat tggcctggtt gcgtccggca ttaaaaatgg ggatctcaat tttaggaata
    39841 aaattccaca tcccgctgct ggcgttaaat aatgacgaca gatcgctgct ggcggttgat
    39901 attccgctgg tcaggctgat agacgggaaa aatgccgcgc gtgcagcacc aatattggca
    39961 ttagccgcca ttaacgcatg ttcagcttcc ataatatccg ggcgctgcaa taagatttgc
    40021 gacgacaagc ccgccggtaa tttaacgctt tgcaggctgt cgctgtttac tgtctgcgct
    40081 tgcggcagct tgccgtagct tcccagtaac agttgcaatg cattattcgc ctgcgccagt
    40141 tccccctgac gtttagcgat gtcgctgcgg gtactttcaa tcaccccgcg agcctgttcc
    40201 agcgccagaa cattactgct acctgtcaac agttgttttt cgacaaacgc atatgactgc
    40261 tgataattac gcagcgtttc ttcggctatt tgcaattgcg catacgccag ctgctgattg
    40321 aaatagcttt gcgcgacatt agaaaccagc agaatatgca ccgcgcgctg agcttcctca
    40381 gtggctaaat aattttgtcg ctcggcttcg ctcatgttct ttaagcgccc gaaaaaatcg
    40441 aggtcaaagc tggcgttaag gccagtagaa aactcccgcg tcgtggctga atcgccttta
    40501 agattgccgc tccagctgcc gctgccctca ccattgagct gtgggtagcg gtcggcatca
    40561 gtcagacgat attgcgcccg tgcctcctgc actttcagcg tcgccatgcg caaatcccgg
    40621 ttattctcca gcgcctcgct aatcagcgtc ttcacctgat tatcaacaaa aaaggtgcgc
    40681 cagcccgcgt tctgatagtt atctgctgcg ttaaccaggc cgttctggct gagtgagaac
    40741 tgctgcggta cgggcattgc cgggcgctga taatccggtg ccagtgaaca accggttagc
    40801 gcaagggcca cacaaaatgg cagaagttta caaggagaca taggctcata atttctggtg
    40861 attttatacc gccaacttta ctcgccaggc tctgattttc cggtgacagg aaaatgacaa
    40921 aattgtcatt ttgccaataa gcgattgcca tctgatcccg ctactctaga attgcccggg
    40981 caacatgcgg aggaaatatg aaactgttga ttgtcgaaga tgaaaagaaa accggagaat
    41041 acttgaccaa agggttaacc gaagccggtt ttgtggtcga tttggccgac aacgggctga
    41101 atggctacca tctggcgatg accggagatt atgacctgat aatcctcgat attatgctgc
    41161 cggacgtgaa cggctgggat atcgtgcgca tgttgcgctc cgccaataaa gggatgccga
    41221 ttctgttgct taccgcgctt ggcaccattg aacatcgcgt caaggggctg gagttggggg
    41281 ccgatgacta tctggtgaag ccgttcgcgt ttgctgaact gctggcgcgg gtgcgcactc
    41341 tcctgcggcg cggggcggcg gtgattatcg aaagtcagtt tcaggttgcc gatttgatgg
    41401 tcgatctcgt cagccgcaaa gtcacccgca gcggcacgcg catcactctg accagtaaag
    41461 agtttactct gctggagttt tttctccgcc atcagggcga agtgctgccc cgctcgctta
    41521 tcgcctcgca ggtatgggac atgaattttg acagcgacac taacgccatt gatgtggcgg
    41581 tgaagcggct acgcggcaaa atcgacaacg actttgagcc gaagctgatt caaaccgtgc
    41641 gcggcgtggg ttacatgctt gaggtgccgg atggtcagta agccatttca gcgcccgttt
    41701 tcgctggcaa cccgcctgac cttttttatc agcctggcca ccatcgcggc gtttttcgcc
    41761 tttgcatgga tcatgatcca ctcagtaaaa gtgcattttg ccgagcagga tattaatgat
    41821 ttaaaagaga ttagtgccac ccttgaacgc gtactaaatc accctgacga aacgcaagcc
    41881 cgacgcttaa tgacgctgga agatatcgtc agtggttatt ccaatgtgtt gatttccctg
    41941 gcagatagcc acggtaaaac ggtgtatcac tcccccggtg cgcctgatat ccgcgagttt
    42001 gcgcgcgatg ccatacccga taaagacgcg cggggcggcg aggtgtttct cctctccggc
    42061 ccgacgatga tgatgccagg ccacggtcac gggcatatgg aacacagcaa ctggcggatg
    42121 atcagtttgc cagttggccc actggttgac ggcaaaccga tttatacgct ctacatcgcg
    42181 ctttcgatcg attttcatct tcattacata aatgatttga tgaataaact tattatgacc
    42241 gcatcggtca tcagcatcct gatcgtcttt atcgtgctat tggcggttca taaaggtcac
    42301 gcgccgatcc gcagcgtcag ccgtcaaatc cagaatatca cctcgaaaga tctcgacgtc
    42361 cgccttgacc cgcagaccgt gccgattgag ctggaacagc tggtactgtc gttcaaccat
    42421 atgatcgagc gtattgagga tgtctttacc cgccagtcca atttctcagc ggatatcgcc
    42481 cacgaaattc gcacaccaat tactaatctc ataacgcaaa cggaaatcgc cctcagccag
    42541 tcgcgcagcc agaaggagct ggaagatgtg ctctactcta atctcgaaga gctgacgcga
    42601 atggcgaaaa tggtcagcga tatgctgttt ctcgcccagg ccgataacaa ccagttaatc
    42661 cccgaaaaga aaatgctcaa cctggcggat gaagtcggca aagtgttcga ttttttcgag
    42721 gctttagcgg aagatcgcgg cgtggagctg cagtttgttg gcgacgagtg ccaggttgcg
    42781 ggcgatccgc tgatgctgcg tcgggcgtta agcaacctgc tttcgaacgc cctgcgttat
    42841 acgccacccg gagaggctat tgtagtgcgc tgccagacgg tcgatcatct ggtgcaagtt
    42901 atcgtcgaaa accccggtac gcccattgcg cccgagcact taccgcgatt gtttgaccgt
    42961 ttctaccgcg ttgacccctc ccgccaacga aaaggcgaag gtagcggcat tgggctggcg
    43021 atagtgaaat caattgttgt cgcgcataaa ggcacggttg cggttacgtc aaatgcgcgg
    43081 gggacaaggt ttgtgattgt gttgccggag agggggtgat tattaagttc aaattcaggg
    43141 cgagggttta cggcgttttt cgctaaattt ccatcattat aaacaagcca cctatcatat
    43201 aaagcaatga ataattaaat cgcgattaat catcattaat ttttatatta ttcacccaat
    43261 ccatttataa agtgaatttg ataaattcct aatttaaaat atccactatt gagccagtat
    43321 ttaacaa
//
LOCUS       DAZKIP010000024        41191 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZKIP010000024 DAZKIP010000000
VERSION     DAZKIP010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41191
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.024"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(145..408)
                     /gene="cedA"
                     /locus_tag="ACHOUK_001586"
     CDS             complement(145..408)
                     /gene="cedA"
                     /locus_tag="ACHOUK_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFP9897058.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            591..2852
                     /gene="katE"
                     /locus_tag="ACHOUK_001587"
     CDS             591..2852
                     /gene="katE"
                     /locus_tag="ACHOUK_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFP9897059.1"
                     /translation="MSQQNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTASGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(2899..3657)
                     /gene="chbG"
                     /locus_tag="ACHOUK_001588"
     CDS             complement(2899..3657)
                     /gene="chbG"
                     /locus_tag="ACHOUK_001588"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFP9897060.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMNFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSHGFSSAFYGEEISEALFLQVLDDSSHRGERSLEVMCHPAFVDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(3670..5022)
                     /gene="celF"
                     /locus_tag="ACHOUK_001589"
                     /gene_synonym="chbF"
     CDS             complement(3670..5022)
                     /gene="celF"
                     /locus_tag="ACHOUK_001589"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFP9897061.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5128..5970)
                     /gene="chbR"
                     /locus_tag="ACHOUK_001590"
     CDS             complement(5128..5970)
                     /gene="chbR"
                     /locus_tag="ACHOUK_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFP9897062.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(5978..6328)
                     /gene="chbA"
                     /locus_tag="ACHOUK_001591"
     CDS             complement(5978..6328)
                     /gene="chbA"
                     /locus_tag="ACHOUK_001591"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFP9897063.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6379..7737)
                     /gene="chbC"
                     /locus_tag="ACHOUK_001592"
     CDS             complement(6379..7737)
                     /gene="chbC"
                     /locus_tag="ACHOUK_001592"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFP9897064.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVVALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(7822..8142)
                     /gene="chbB"
                     /locus_tag="ACHOUK_001593"
     CDS             complement(7822..8142)
                     /gene="chbB"
                     /locus_tag="ACHOUK_001593"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFP9897065.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(8441..8779)
                     /gene="osmE"
                     /locus_tag="ACHOUK_001594"
     CDS             complement(8441..8779)
                     /gene="osmE"
                     /locus_tag="ACHOUK_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFP9897066.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            8981..9808
                     /gene="nadE"
                     /locus_tag="ACHOUK_001595"
     CDS             8981..9808
                     /gene="nadE"
                     /locus_tag="ACHOUK_001595"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFP9897067.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREACIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPQQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            10038..10925
                     /gene="cho"
                     /locus_tag="ACHOUK_001596"
     CDS             10038..10925
                     /gene="cho"
                     /locus_tag="ACHOUK_001596"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFP9897068.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(10885..11460)
                     /gene="ves"
                     /locus_tag="ACHOUK_001597"
     CDS             complement(10885..11460)
                     /gene="ves"
                     /locus_tag="ACHOUK_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFP9897069.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADHFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(11663..12148)
                     /gene="spy"
                     /locus_tag="ACHOUK_001598"
     CDS             complement(11663..12148)
                     /gene="spy"
                     /locus_tag="ACHOUK_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFP9897070.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(12478..13446)
                     /gene="astE"
                     /locus_tag="ACHOUK_001599"
     CDS             complement(12478..13446)
                     /gene="astE"
                     /locus_tag="ACHOUK_001599"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFP9897071.1"
                     /translation="MDNFLALTLTSKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFKKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(13439..14782)
                     /gene="astB"
                     /locus_tag="ACHOUK_001600"
     CDS             complement(13439..14782)
                     /gene="astB"
                     /locus_tag="ACHOUK_001600"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFP9897072.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNKASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVVRQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTQEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(14779..16257)
                     /gene="astD"
                     /locus_tag="ACHOUK_001601"
     CDS             complement(14779..16257)
                     /gene="astD"
                     /locus_tag="ACHOUK_001601"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFP9897073.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAAFPRWARLSFAERQAVVERFAGLLERNKGELTAIIARETGKPRWEAATELTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGFPPGVLNLVQGGCETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRVLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVFTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     IRMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(16254..17288)
                     /gene="astA"
                     /locus_tag="ACHOUK_001602"
     CDS             complement(16254..17288)
                     /gene="astA"
                     /locus_tag="ACHOUK_001602"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFP9897074.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(17285..18505)
                     /gene="astC"
                     /locus_tag="ACHOUK_001603"
     CDS             complement(17285..18505)
                     /gene="astC"
                     /locus_tag="ACHOUK_001603"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFP9897075.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNESVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNIINHRYGLFNEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAVACEHFVSRGSS"
     gene            18951..19757
                     /gene="xthA"
                     /locus_tag="ACHOUK_001604"
     CDS             18951..19757
                     /gene="xthA"
                     /locus_tag="ACHOUK_001604"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFP9897076.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDEEEAQRRIIMAEIPSPLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRENPVLIMGDMNISPGDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMERLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            19924..20634
                     /gene="ydjX"
                     /locus_tag="ACHOUK_001605"
     CDS             19924..20634
                     /gene="ydjX"
                     /locus_tag="ACHOUK_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFP9897077.1"
                     /translation="MNAERKFLFACLIFALAIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPFLGTLLSLIAATLASSCSFLLARW
                     MGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRNPLTHPKNEG"
     gene            20639..21316
                     /gene="ydjY"
                     /locus_tag="ACHOUK_001606"
     CDS             20639..21316
                     /gene="ydjY"
                     /locus_tag="ACHOUK_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFP9897078.1"
                     /translation="MLQHYSVSWKKGLTALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKTYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPANNTLAT
                     VTFKITE"
     gene            21331..22038
                     /gene="ydjZ"
                     /locus_tag="ACHOUK_001607"
     CDS             21331..22038
                     /gene="ydjZ"
                     /locus_tag="ACHOUK_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFP9897079.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGSLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            22038..22586
                     /gene="ynjA"
                     /locus_tag="ACHOUK_001608"
     CDS             22038..22586
                     /gene="ynjA"
                     /locus_tag="ACHOUK_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFP9897080.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQELTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            22596..23762
                     /gene="ynjB"
                     /locus_tag="ACHOUK_001609"
     CDS             22596..23762
                     /gene="ynjB"
                     /locus_tag="ACHOUK_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9897081.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTDWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALNEAPNDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTFRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            23735..25270
                     /gene="ynjC"
                     /locus_tag="ACHOUK_001610"
     CDS             23735..25270
                     /gene="ynjC"
                     /locus_tag="ACHOUK_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFP9897082.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCTRLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDKLGIGLGFTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLGYLLWCTWRRTIPRVDGVRKP
                     ATPLLPGITLASFLPLTSVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNMDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAALALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            25270..25923
                     /gene="ynjD"
                     /locus_tag="ACHOUK_001611"
     CDS             25270..25923
                     /gene="ynjD"
                     /locus_tag="ACHOUK_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP9897083.1"
                     /translation="MLCVKNVSLRLPESCLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            25990..27297
                     /gene="ynjE"
                     /locus_tag="ACHOUK_001612"
     CDS             25990..27297
                     /gene="ynjE"
                     /locus_tag="ACHOUK_001612"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFP9897084.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRSSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNGKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAARGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(27306..27926)
                     /gene="ynjF"
                     /locus_tag="ACHOUK_001613"
     CDS             complement(27306..27926)
                     /gene="ynjF"
                     /locus_tag="ACHOUK_001613"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFP9897085.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPQQNALAGGWLLFAFIGTGSSFLAFAALAAKYQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            28013..28420
                     /gene="nudG"
                     /locus_tag="ACHOUK_001614"
     CDS             28013..28420
                     /gene="nudG"
                     /locus_tag="ACHOUK_001614"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFP9897086.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(28386..28658)
                     /gene="ynjH"
                     /locus_tag="ACHOUK_001615"
     CDS             complement(28386..28658)
                     /gene="ynjH"
                     /locus_tag="ACHOUK_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFP9897087.1"
                     /translation="MSRALFAVVLAFPLIALANPHYGPDVEVNVPPEVVSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            28894..30237
                     /gene="gdhA"
                     /locus_tag="ACHOUK_001616"
     CDS             28894..30237
                     /gene="gdhA"
                     /locus_tag="ACHOUK_001616"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFP9897088.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKSSRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(30354..31394)
                     /gene="ynjI"
                     /locus_tag="ACHOUK_001617"
     CDS             complement(30354..31394)
                     /gene="ynjI"
                     /locus_tag="ACHOUK_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFP9897089.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKTMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLEEQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANHSSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(31522..33483)
                     /gene="topB"
                     /locus_tag="ACHOUK_001618"
     CDS             complement(31522..33483)
                     /gene="topB"
                     /locus_tag="ACHOUK_001618"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFP9897090.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRYLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLISRQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLIKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            complement(33488..34531)
                     /gene="selD"
                     /locus_tag="ACHOUK_001619"
     CDS             complement(33488..34531)
                     /gene="selD"
                     /locus_tag="ACHOUK_001619"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFP9897091.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(34648..35199)
                     /gene="ydjA"
                     /locus_tag="ACHOUK_001620"
     CDS             complement(34648..35199)
                     /gene="ydjA"
                     /locus_tag="ACHOUK_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFP9897092.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            35360..37216
                     /gene="sppA"
                     /locus_tag="ACHOUK_001621"
     CDS             35360..37216
                     /gene="sppA"
                     /locus_tag="ACHOUK_001621"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFP9897093.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKADKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKAKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            37332..38042
                     /locus_tag="ACHOUK_001622"
     CDS             37332..38042
                     /locus_tag="ACHOUK_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310500.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HFP9897094.1"
                     /translation="MNKAPSLIAAIVLGLGISACGYFVGDGVKHLKTNNRYVNVRGLS
                     EKEVRADTAELTIAINFKGNVPGELFPKLEEAQKKIVAELNAQGINEKEIILGQWTSK
                     RTDSFYLKDDPTMPRYNADGSVTIKTHNVAAVEKVVAKLNELQVATDGAIAESKVAYR
                     FNGIGALRAEMIAAATKDARNAALQFATDSGSQVGSISDASQGVFQIFASGSDEDDPT
                     AINKTVRVVTTVTYALQD"
     gene            38174..39190
                     /gene="ansA"
                     /locus_tag="ACHOUK_001623"
     CDS             38174..39190
                     /gene="ansA"
                     /locus_tag="ACHOUK_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFP9897095.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            39201..39842
                     /gene="pncA"
                     /locus_tag="ACHOUK_001624"
     CDS             39201..39842
                     /gene="pncA"
                     /locus_tag="ACHOUK_001624"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFP9897096.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(39935..>41191)
                     /gene="ydjE"
                     /locus_tag="ACHOUK_001625"
     CDS             complement(39935..>41191)
                     /gene="ydjE"
                     /locus_tag="ACHOUK_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9897097.1"
                     /translation="GFLSYSGNVVLAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGG
                     FIGDYFGRRRAFRINLLIVGIAATGAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFT
                     EFIPATVRGKWSARLSFVGNWSPMLSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSG
                     KYFIESPRWLAGKGQIAGAECQLREVEQQIEREKSIRLPPLTSYQSNSKVKVIKGTFW
                     LLFKGEMLRRTLVAITVLIAMNISLYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAP
                     VGIFIAALIIDHFPRRLFGSTLLIIIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVC
                     FASAVYIPELWPTHLRLRGSGFVNAVGRIVAVFTPYGVAALLTHYGSITVFMVLGVML
                     LLCALVLSIFGIETRKVSLEEISEVN"
BASE COUNT        10213 a        10846 c        10147 g         9985 t
ORIGIN      
        1 acaattaaag aaaaaagatc acttatttat agcaatagat cgtcaaaggc agctttttgt
       61 tacaggtggt ttgaatgaat gtagcaatga aatagagaat ttcaggccat gtaactcccg
      121 gcaaaaccgg gaggtacgta atctttactc agtcacttcc ccttcctggc ggatctgatt
      181 tgcccaacgc tgggcggatt caggcacagt aaacgccggt gagcgcagaa atgactctcc
      241 catcagtaca aacgcaacat atttgccacg cagcatccag acatcacgaa acgaatccat
      301 ctttatcgca tgttctggcg gcgcgggttc cgtgcgtggg acatagctaa taatctggcg
      361 gttttgctgg cggagcggtt tcttcattac tggcttcact aaacgcatat taaaaatcag
      421 aaaaactgta gtttagccga tttagcccct gtacgtcccg ctttgcgtgt atttcataac
      481 accgtttcca gaatagtctc cgaagcgggg tctggctggt ggtctatagt tagagagttt
      541 tttgaccaaa acagcggccc tttcagtaat aaattaagga gacgagttca atgtcgcaac
      601 aaaacgaaaa gaacccacat cagcaccagt caccactaca cgattccagc gaagcgaaac
      661 cggggatgga ctcactggca cctgaggacg gctctcatcg tccagcggct gaaccaacac
      721 cgccaggtgc acaacctacc gcctcaggga gcctgaaagc ccctgatacg cgtaacgaaa
      781 aacttaattc tctggaagac gtacgcaaag gcagtgaaaa ttatgcgctg accactaatc
      841 agggcgtgcg catcgccgac gatcaaaact cactgcgtgc cggtagccgt ggtccaacgc
      901 tgctggaaga ttttattctg cgcgagaaaa tcacccactt tgaccatgag cgcatcccgg
      961 aacgtattgt tcatgcacgt ggatcagccg ctcacggtta tttccagcca tataaaagct
     1021 taagcgatat caccaaagcg gatttcctct cagatccgaa caaaatcacc ccagtatttg
     1081 ttaggttctc taccgttcag ggtggtgctg gctctgccga taccgtgcgt gatatccgtg
     1141 gctttgccac caagttctat actgaagagg gtatttttga cctcgttggc aataacacgc
     1201 caatcttctt tatccaggat gcgcataaat tccccgattt tgttcatgcg gtaaaaccag
     1261 aaccacactg ggcaattcca caagggcaaa gcgcccacga tactttctgg gattatgttt
     1321 ctctgcaacc tgaaactctg cacaacgtga tgtgggcgat gtcggatcgc ggcatccccc
     1381 gcagttaccg caccatggaa ggctttggta ttcatacctt ccgcctgatt aatgccgaag
     1441 ggaaggcaac gtttgtacgt ttccactgga aaccactggc aggtaaagcc tcactcgttt
     1501 gggatgaagc acaaaaacta accggacgtg acccggactt ccaccgccgc gagttgtggg
     1561 aagccattga agcaggcgat tttccggaat acgaactggg cttccagttg attcctgaag
     1621 aagatgaatt caagttcgac ttcgatcttc tcgatccaac taaacttatc ccggaagaac
     1681 tggtgcccgt tcagcgtgtc ggcaaaatgg tgctcaatcg caatccggat aacttctttg
     1741 ccgaaaacga acaagcagca ttccatccag gtcatattgt tcctggtctg gacttcacca
     1801 acgatcccct gttgcaggga cgtttgttct cctataccga tacacaaatc agtcgtcttg
     1861 gtgggccgaa tttccatgag attccgatta accgcccaac ctgcccttac cataatttcc
     1921 agcgtgacgg catgcatcgt atggggatcg acactaaccc ggcgaattac gaaccgaact
     1981 cgatcaacga taactggccg cgcgaaacac cgccgggacc gaaacgcggc ggttttgaat
     2041 catatcagga acgcgtggaa ggaaataaag ttcgcgagcg cagtccatct tttggcgaat
     2101 attattccca tccgcgtctg ttctggctaa gtcagacgcc attcgagcag cgccatattg
     2161 tcgatggttt cagttttgag ttaagcaaag tcgttcgtcc gtatattcgt gagcgcgttg
     2221 ttgaccagct ggcgcatatt gatctcactc tggcccaggc ggtggcgaaa aatctcggta
     2281 tcgaactgac tgacgaccag ctgaatatca ccccacctcc ggacgtcaac ggtctgaaaa
     2341 aggatccatc cttaagtttg tacgccattc ctgacggtga tgtgaaaggt cgcgtggtag
     2401 cgattttact taatgatgaa gtgagatcgg cagaccttct ggccattctc aaggcgctga
     2461 aggccaaagg cgttcatgcc aaactgctct actcccgaat gggtgaagtg actgcggatg
     2521 acggtacggt gttgcctata gccgctacct ttgccggtgc gccttcgctg acggtcgatg
     2581 cggtcattgt cccttgcggc aatatcgcgg atatcgctga caacggcgat gccaactact
     2641 acctgatgga agcctacaaa caccttaaac cgattgcgct ggcgggagac gcgcgcaagt
     2701 ttaaagcaac aatcaaggtc gctgaccagg gtgaagaagg gattgtggaa gctgacagcg
     2761 ccgacggtag ttttatggat gaactgttaa cgctgatggc agcacaccgc gtgtggtcac
     2821 gcattcctaa gattgacaaa attccggcgt aaatatggat aaggtcacgt cccctgggga
     2881 gtgaccttac caccttactt acacatcaag ataactaccc aaccgataac cacgctcagc
     2941 aattgcatat ttcaacgacg ctgatgtcag cacttccagc tccgttaacc gtgggaaaca
     3001 gtaagcactc tggcgaatcg tattatcgac aaacgccgga tggcacatga cttccagcga
     3061 gcgttccccg cgatggctgg aatcatcaag cacttgcagg aacagtgcct cgctaatctc
     3121 ttcaccataa aatgcgctac tgaatccgtg cgaactgcgc agattagccg gtaaatcacc
     3181 agcgttagat agcggctgac ggtcgatgcg caacgcaatc ccctcttcag ccgcaaacct
     3241 ggcaacaata gggaaaatct gcgggaacat atgcacatga tgatggctat caagatgcgt
     3301 aggtttgcgt ccaaatagct caatgaaacg caaatactga ctggcaagct cctgagtaat
     3361 ttcttccagc ggtaaagcat cttcttctgc caactgccag atccattttc ccagcacacc
     3421 atcgcgggtt aaccccggca tagctgtcag tggcttgccc atagtaagga caaagttcat
     3481 ccctatggcc agactcggtt catcacgact caactgcacc gcatggtcaa tagcctgccc
     3541 attcaccagc gccgtcgtcg atgtgacaat cccattgcga caggcctcga taatgccgta
     3601 gttctgacct ttgcttaagc caaaatcatc ggcattaaca atcagtaagc gttccataat
     3661 cagcctcggt taatgtgctt ttttaagctc tgcgatgcag tcggcaaaat ttggcagcca
     3721 tttctcgtgc gccagaatca tctcgcgtgc cagcagctca gcatcgcgat cggaatgcac
     3781 caacggacta aggtttagcg ccagtaacac atcgttaaat tctccgctaa gtgcggcgtt
     3841 gctggcagca atctcgaagc ctttaatggt gtgaatcagc cccatcactt tatcatcgaa
     3901 atgcgtaatg cgcggatgtg gcgtcgcgcc atcgcgcccc agcgtacagg tcatttctac
     3961 cgcccagtct gccggaatat tatcaatatg cccatgatgc gggatattaa cgtaatgttc
     4021 tgcttgctta tcgttgtaga tagcgttgat cacttcgcac gctgcatcag agtaataagc
     4081 cccaccgcgc tgttccagtt ctttcggctt aactttcaac tccgggtttt tatacagctc
     4141 aaaaagttgt ttctcgactt tctgtactac ctgcgctcgt gcgccgcctt tgtagtattc
     4201 gcccatttca atagccagca tctctttttg cttgaagtaa taaagcaaat aagaacacgg
     4261 caacagattc agagaacgaa ttaagccttc gctaaatggc agatcgaaaa tatttttaac
     4321 gccagatgct tttaactgcc cggatgccac accatcaagc aattcggcaa agcgcgactt
     4381 gccatttacc agcacatcct taatgaacac catatggttg aggccgaaca gatcgataga
     4441 taaatcatcg ctgtctttca gcatcagaac atcgcgaata aacatcttca tgccgatcgg
     4501 aatattacac acgccgataa agcgtttaaa tccggtatga cgataaacgg cttcagtgac
     4561 cattcccgcc gggttagtga agttaatcac ccatgcattc ggacaaagtt cttcgacatc
     4621 ttttacgatg tcaaaaatca ccggaatggt acgcagacct ttaaacagac cgcccgcgcc
     4681 gttggtttcc tgaccaagat aaccatgact taatggaata cgttcatcca gttcacgcgc
     4741 cggtaattgg ccaacgcgca gttgggtagt aacgaaatca gcatctttca atgcttcgcg
     4801 gcgatccagc gttttataaa gcttcatcgg gacgccagcg ttatcaatca tccgttggca
     4861 gagatcgaaa ataatatcca gtttagcttt gccaccttcg acatccacca gccataattc
     4921 gctgaccggc aattcgtgat aacgcttaat aaatccttcc agtaactccg gggtatagct
     4981 gctcccgcca ccaatagtga cgacttttaa tttctggctc ataatttctc ccttcagtac
     5041 agaatactga tatctggcat atctgccccc cccggacata aataatccag caacaggaca
     5101 gatatgtgaa ttgtcaggta taacgactta ctgattaaat tcagtcaatt tcttacgata
     5161 gctcttaggc gtaaaggacg ttaatttctt aaacgtttta ataaacaaac tcgggctact
     5221 ataaccggcc tcaaacgcaa tatccgtcac tgaatagttg gtcatttcca gttgtttttt
     5281 ggcaaaatta atacggattt cattaataat ctgcattggc gttttgccat aatatcgttg
     5341 agtcgcccgc gtcaaatatt cctgtgattt ggctgacaac gtcaccatat tctccagcgc
     5401 cgattcacta aactgctctt tatcatgcat cttttctacc gtacttttca gccactgcgg
     5461 tacatcatca atcacctgtt cttcgcggta atgacgtaaa cggttaatga cataaaaagt
     5521 aaccatctca acaaactctt ccagccctgt ttcgcggaaa ttcaatgaag aaatcactgt
     5581 ttccacatag gtgagaaaag cgttattggt ccggtatacc tgcgaagcta caaagcaata
     5641 aggcaacaat ggcaggtaat gctgctcaaa aaagcgttta ctgatcccaa cgttcaatat
     5701 gcgcgtggca ccaaactcat aaaaactttg atggtgcgaa cctaacggaa taaaaacaaa
     5761 atcgccccgt tccagtaaca cgcgcttacc gttaatctcc tggaaataac gcccggttaa
     5821 taccagagta aattcataat agtcgtgctg atgcagtccg ctgatactct cagttttgtt
     5881 atagataaac acatggaagt ttttgccatt aaacaactgc tgttctcggg cagtggcaat
     5941 ttccggcgcg ttaatcactg gctgcatcat cgactcctta tgccttcagt ttttcatgaa
     6001 gctcaattaa ttcagtaatc agttcacgcg caagcatgga cgtcattaaa tgatcctgag
     6061 cgtggaccag caccagactc actttcatct taccttcgcc cgcatcgcct tcaatcagtt
     6121 tcgtctgtac cagatgcgct tcattcaatg ccatccgtga ctgatccatc atggcttttg
     6181 ctgcggcaaa atcgccctgc ttcgcctgtt tcagtgcggc atacgccagg ctgcgcgctt
     6241 gtccggagtt gatgataagc cccatcacca cttcttccag ctcttcagct tccgtttgcg
     6301 tatcgggaat attatcgaga tccatcatac atcgttcctc tcttttctta ccggcacgat
     6361 tacccgtacc ggcatcgatt aaaatttcag ggcgttagcg atatcttctt cgctctcttc
     6421 tttatcaatc gcattttgtg ctttgttagc caccacaaca aagggcagat aaattaacgt
     6481 tgcgatacca aggttgaaga gtgcaaccag caatgcgacg acgctaccgt tggtgttaaa
     6541 gaaggctccc agaccggttg gcatggtcca cggtgcaata ttggtcaccg gaggaataat
     6601 gcccatgtag tacgctgcca gggtgattgc cgccagaatc ggttgtacca gtacaaacgg
     6661 gataaacatc accgggttca tgataattgg cagaccaaac agaatcggtt cgttaatctg
     6721 gaagatgccg gacggcagcg ccagttttgc cacctgacga taatcagcac ggcgagaggc
     6781 aataaagata gccaggatca ggcctaaagt cgcaccactg cctccaagga aaataaagga
     6841 gtccagcatc ggcttggccc agatatggaa ggtcttaccg gctgccagcg ccgcttcgac
     6901 ggaaccatat tgctgatagg tcgcgatatt ttccagcgcc cacggcgtca taatgccgtt
     6961 gtccagtgcg gtcagcgcca gcgcgccatg aataccgaag aaccagagca gtggaacaaa
     7021 gatcacatag gcccagccca ccacgctacc caacgatgcc agtggggttg agatggtatc
     7081 cataatgatc tgatggaagt tggtgcccca ggtattcaac gcccaggcaa taatccccat
     7141 cacggaaaga ataataaagc cgggaattaa tgccgagaag gaacgcgata ctgaagcagg
     7201 tacgctgtcg ggcagtttaa tgacccaatt gcggcggaca ataaaggtaa acatttctgc
     7261 caccaccagg ccaataataa tcccggagat gatattcgcc ccacctaacc agtttgcgcc
     7321 aaccgcatag gcctcaccga cactatatgg ggtgacggtc ataaatgctg caacggataa
     7381 caacccagcc gccagcgcat cgactttacg ctcttctgcc agcgccatgc caataaagaa
     7441 cggtgccatt aaagacatta tccctaatgt tccgttatat acgttgccgc caataccttt
     7501 cagaccatta agtgtttcaa tggttgaggc gtcgaggcga atacctaagg aataaaaaaa
     7561 cgacccctcc ccaaagctta gaaaaacgtt gttaattaat acaaacatgg cccccgcaag
     7621 ggttaacggc attaagcgaa taaagccatt tttgattgca ttaacgtgtg gctgctttcc
     7681 tattttaact gcaaaaggga ggagtacctt ttcaagcgat gcaataacat tactcataga
     7741 aaaataccct taaaaaccgc aatttaaata ttgcggtatt gatttatgaa ataactcttt
     7801 gacgggaaaa tttaaaataa attaatttgc tgcggctttt ttaatcgctg caaccgcagc
     7861 cttaagcacg cctaaaccat cgactttgcc ataaagcagc gagtcaatta cttcaaccgg
     7921 tttgttgggt aacaaacgct ggatttcggg caacatataa gcaatctgcg gccctaataa
     7981 cacgacatcg gcattctgac ctttttcacc agccagtgtt tccggaaatg cttcaataat
     8041 gaccggaact tcatattttt ctgcctgtgc gcgcattttt gataccagta aagaggtaga
     8101 catgcccgca gaacaaaaca gataaatgtg tttcttttcc ataaaactgc cctcatcgac
     8161 gattatctgt cagccagaca ctccgcaagc cttaacctgc ttccatgctc tgggtaactt
     8221 gcgaaaccaa catgactttt tttgtggctg aaaggagtat acggtataga cctgagcaat
     8281 agtatttctt tgaccttata aattgtctct ccttgacctg cggttatgac cctcttcaca
     8341 tttcgggcaa ataattcgcg aagataatta agaactcagg ccaaaaaaaa ccggcgcaat
     8401 ggccggtttc cgttgttact caagctttca gacgaagtga ttacttcgca gcctgtggat
     8461 cagtgtcgta ttcagcacag gtctgataac cggagttgat gacatgtccg gtatcatcta
     8521 acgcgacaaa gtaggtttct gctttaccat cacgttgacc caggatgtag gtctggcaag
     8581 taccacgagc atggatcatg ctcacttcag acgaaggttt acccgcaatt tgtgcaacct
     8641 gcgcccggct catgcctttt ttgacgtctt tcaccacagg ctgtacaaac tggtctttgg
     8701 tacgatcata agccgtacaa cccgccagca tggttaatac cgccgctgca ctcagaattc
     8761 ctgccatatt cttgttcata ttccgtccta ttgtttatca gcgtgttaga taagcctgga
     8821 atacattggg cgctttttca agcccatgaa cgaaacggct ccgctttcag aggattcctg
     8881 tatgacgttt taaccaccat tcagcccgct gtcgcttgtc gtttcagtag caacgggtta
     8941 gctttaagga agttttgtct tttctgtctg gaggggttca atgacattgc aacaacaaat
     9001 aataaaggcg ctgggcgcaa aaccgcagat taatgctgaa gaggaaattc gtcgtagtgt
     9061 cgattttctg aaaagctacc tgcaaactta tccgttcatt aaatcactgg tgctcgggat
     9121 cagcggcggt caggactcca cgcttgccgg taagctgtgc cagatggcga ttaatgagct
     9181 gcgccaggaa accggcaacg aatcactgca atttattgcc gtacgcctgc cctatggtgt
     9241 tcaggccgac gaacaagatt gccaggatgc cattgccttt attcaaccgg atcgcgtatt
     9301 aaccgttaat atcaagggcg cggtattggc tagcgagcag gcattgcggg aagcatgcat
     9361 tgaactgagc gattttgtcc gtggcaatga aaaagcgcgt gagcggatga aagcacaata
     9421 tagcattgcg ggtatgacca gcggtgtcgt ggtgggcacc gatcatgcag cagaagccat
     9481 taccggattc ttcactaaat atggtgacgg cggtacggac attaatccac tgtatcgtct
     9541 caacaaacgt cagggtaaac agttactggc agcattaggt tgcccggaac acctttataa
     9601 gaaagcgcca acggccgatc tggaagatga tcgcccttct ctgccagatg aagtggcact
     9661 cggcgtgacc tatgacaata tcgacgacta tctggaaggg aaaaacgtac ctcaacaggt
     9721 cgccagaaca atagagaact ggtatctgaa aaccgaacat aaacgccgtc cgccaattac
     9781 cgttttcgat gatttctgga aaaagtaata atttgcacct attggattgt gcaaaaaaga
     9841 gtaatttgtt cacgccggat gcggcgtgaa cgccttattc gacctacaaa actatgcaaa
     9901 ttcaatacat tgcaggagtc gaataggcct gacaggcgta gcacgtcaga cggtgtaacc
     9961 tttgtcatcg acccgcctct tttttaatcg cttcccggct gttacactgg atagataacc
    10021 agcattcgga gtcaacagtg gtacggcgtt taacttctcc gcggctcgaa tttgaagctg
    10081 cggcaattta tgaatatccc gaacatttac gttcattcct taatgactta cccacccgac
    10141 caggggtgta tctgtttcat ggcgaaagtg acaccatgcc gctctatatc ggcaaaagcg
    10201 ttaatatccg cagtcgggtg ctttctcatt tacgcacccc ggatgaagcc gccatgctac
    10261 ggcaatcccg gcgaattagc tggatctgca ccgcaggcga aatcggcgct ctgctccttg
    10321 aagcgcgatt aatcaaagaa caacagccgc tgtttaataa acggttgcgc cgcaatcgcc
    10381 agctctgtgc cctgcaatta aatgaaaagc gcgtcgatgt ggtgtatgcc aaagaggtgg
    10441 atttttcacg agcccccaac ctgtttggcc tgtttgccaa taggcgcgca gctttgcaag
    10501 cattgcagag catcgctgat gaacaaaaac tttgttatgg tctgttggga ctggaaccgt
    10561 taagtcgcgg tcgtgcgtgt tttcgttcag cgctaaaacg ttgcgccgga gcatgctgcg
    10621 gtaaagagag ccatgaggaa catgcgctac gcttgcgcca gtctctggag cgtttgcggg
    10681 tggtgtgttg gccttggcaa ggggcggtgg cgctgaaaga acagcacccg gaaatgactc
    10741 aatatcatat tattcaaaac tggctgtggc tgggggcggt taattcgctg gaagaagcga
    10801 caacgttaat tcggacaccc gccgggtttg atcacgacgg ttataaaatt ctttgtaagc
    10861 cgctgctttc cggtaactat gaaattactg aacttgatcc ggcgaatgac cagcgagcca
    10921 gttgatttca ctgaacaaca gtttaccttg cggttgtaaa agccgcaggg tgtgcctgcc
    10981 atcgaaccag cacgcgccct ggtcggtagt gagcagtttg tcgcccagtt gccacgcccc
    11041 gttaatcaca aacaccaccc cgccgcgtga gccaaaggta gtaaacgtac gttcggcaat
    11101 tctgactttc gccttacaga catccaggcg cgtcataata ttgaaatcca tcgacatctg
    11161 ccctgccgtc agtttcgctt ttaccacctg gtccgccgca aaggcaaaag gctgtaacgg
    11221 ttttaaggta tggttaaagt ggtctgcgct ttcaaggaac atctcgccgc cttccagcaa
    11281 cgtcactatc ctttccatac caggaaacaa agaaaactcc ccattcgccg cgatggatgc
    11341 aatgctggca cgccagtaaa aatcacgttt tgccggtggg aaagtgcaaa tttcgcgcgt
    11401 ttcaccggca gcgtttcgcc acaaattcac cgacatttta cgcatatcaa agtattccat
    11461 actcgctccc ggcccgttct tttcattgtt atcgttcagg taaccgtgga tggttatcgg
    11521 caactcgggt gatttacgtt agtggtgatc aggctttttt tacaactgtt tgagaagaga
    11581 aaagaaaacc gccgatcctg tccaccgcat tactgcaagg tagtggacaa gaccggcggt
    11641 cttaagtttt ttggctgaaa gattattcag cagttgcagg cattttacct tttgccgctg
    11701 gacgttctgt cagacgcttc tcaaaattag cattaaattg ctttttctgt tccggcgtca
    11761 ggatgttgta aattttgttc tgggtttcca tgtgcgccag catgttagct ttgcgctgtt
    11821 cttccatttt tgcgatctgc gcttcagctt ttgctttatc gaaggtatcg ctggcaatga
    11881 tgtcatgcat tgcgcgacgt tcttccagcg gcggacgttt catctggtca cgctggcctt
    11941 tcatgatttc gcgaatctgc tgtttctgcg cgtcggtcag gttcaggtct ttgaacatca
    12001 tgtcctgatg cggaccgaac ttgcctttgt ggtgcatcat cggcttcgcg tcagccggtg
    12061 ctgcggtagt ggtgtctgcg gcatgggcca ggttagccgc gccaagagcc agggtagagg
    12121 caacaaacag tgcagttaat ttacgcatat tctatatcct tcctttcagt tatttattac
    12181 ggctttctta agtagcgtgc cgtgttgacg agattaactt tactgacttt agcgtcaatt
    12241 aatcagagca acggtaaaac aatgaaagtg taaaaaacac ttttgcgcca attatggaga
    12301 aaaaaagaaa atttgatgga gagtgatgag agaatattac aacaccatga ttttgcagag
    12361 attatgaaga actataccgg atgactggtg ataaataaag caaataacca ggattaatct
    12421 gtattaattt ataagaaggt aacttaatac ccgcagaaag atttctgcgg gtaagtatta
    12481 gcttattttt tcgagcatta atcccgcgcg taatcccagc gctaccaacg gattagggaa
    12541 taacacatac tctacatcat gggttacggt aaaacgttcc tcaccgtcct gcgccagcaa
    12601 cgttcctttc ttaaacggca taaaattcag cgtgtcactt gccatatgca tttcgaagga
    12661 cggcgagtgg cgagtaattt gcgaaaccac ccgataacgg agcggcggtg ttctcacgat
    12721 accgacactc tcaccagata gcagcgcagc gattgcgctg gcagttactg caaactggcg
    12781 aagatcgttt tgcccaaagg gcaacgcttt gccaagttcc agcgtacagg ccagcgcgcc
    12841 aaaatgtctg gcgctgaaat gggtaaacgt accaccaggc tcctgatgga acaccagcgc
    12901 ctccagcccc gccgcaccca gccacgtcag gaatttctcg tcccagggaa tgtcgcgttg
    12961 cggtaataca ccgaactgcg gatgcaagga gccacgaatt gcggtatgta gatcaaggtg
    13021 ccagcgcaca gattctttgc cctggtcata aaaatcttcc aggcactgtt ccagttcgcg
    13081 cgcccgacag gtttccccgc tttcagcaaa cagctgccaa cgaccgccaa acattcggtt
    13141 catatcgcta tggcaataac gtttcccttg cttcagcgca ggaggattcc cgaggatcac
    13201 cagcaaccgc caccgtaacg ggatctcgcc gtgagatatc gcgccaagca atgcatcaag
    13261 catctccaca ggtgccgtct cattaccgtg gatccccgca gaaatcacca gagcgccttg
    13321 cggtggcgtt aatggcgtca gttccagcac accatcgccc agccagcgcc agcgaacgcc
    13381 gttgatttct cgctcggtga tgacaggttt tttactcgtt aaggtcagag caagaaaatt
    13441 atccattgcc ccctccctcg cgctggaacg gataaaccga accgagattc agtaattggc
    13501 tcaatacatc cagtgcttcc cgcccttcgc gcagcaattg cgggtcggcc agatcggcag
    13561 cagtaagacg atcgcggtag taacgatcca cccagtcatt gagcgcatta aacaaggtat
    13621 cgttcatcat caccgccgga ttcaccgccc ggcgttcttc ttgtgtcaat accacccgca
    13681 accgcaggca cgccgggcca ccgccattcg ccatgctttc acgtaaatca aagactttta
    13741 gttcgctaat cgggttgtca gcggcaagga gttcattgag ataaccccat actccggcgt
    13801 gttcccgaca ctcctgaggc agcaccaaca tcatggaacc atcatcgcgg ctcaacagtt
    13861 ggctgttaaa cagatatgta gacaccgcat ccgacacgga aacctgagtt gccggaactt
    13921 ctatcgccat aaaaccgttc acccgcgcac gcaggtttgc cagtaactgt gactggcgag
    13981 cgaacgcctg ttggtggcaa aacagcacct ggcggttact cacggcaatc acgtcattat
    14041 gaaaaacgcc ctggtcgata acgtccgggt tttgctgggc gaaaatcacc tgttggggat
    14101 tcacctgatt cagccttgcc accgcctcgc tggcttcgcg agtctgtcgc gccggataac
    14161 gggaaggccg ggtatcattg ccctcttctc gcccgtagac aaaaagttgc atacccggtt
    14221 caccgtaatg accgccgaga cgattgtggt ttgccgcccc ctcatcaccg agcaaggcta
    14281 cctgcggcaa cgccgaatgg acactaaatt tctcttcgtc gttaaaaatc gcttttaaca
    14341 gcgattcagt gacgggcgct tccagcgaac ggtgaaattt attgttcagg ttagcaacgg
    14401 tgagatgcac tttgccatcc agcgtatcgg cagatggcgc gatcgttgcc gcattggcta
    14461 cccacattgg cgaagcggaa ctgacgctgg aaagccagtg cggtgcctgg cgtacaacct
    14521 tttccagtac ctgctcatcg ctaccactga atcccaactg acgcagcacc ggaataaacg
    14581 gacgctcgtg aggcgggatc acggcctggg ggaatcccgc atcggcaagg gttttcattt
    14641 tcagtaagcc ctgttttgcc gccagtcgcg ggttagagac ctgaaaacgg tgacgggtag
    14701 aggctttatt accaaacgac aggcccgcgt aatgatgcgt cagccctacc agcccgtcga
    14761 aattaacttc ccaggcgttc atcgcaccac ctcatcggaa aaatccagcc cggggttaag
    14821 cgttgcgggc aaagttaacg agtccgactc caggctcgcc ataggccatg cgcagtaatc
    14881 tgcggcatac caggcgctgg ggcgatggtt accggatgca ccaatgccgc cgaatggcgc
    14941 ggtactggca gcaccagtaa gcggtttgtt ccagttaaca atccccgccc gcgcctccag
    15001 caacagttga tcgaattttt cccgctcggg ggaaaccaga ccgcaagaga gtccgaagcg
    15061 agtgttattc gccattcgaa tcgcttcctc gaaagaatca taacgccaga cgcgcaataa
    15121 cggtccgaac acctcttcat ctggtacgcc agcaacgcct gtcatttcaa tgatccccgg
    15181 cgtcagcaac gatgtccctg cttgtaataa gcgcggcgca agcagggttc gtccacccat
    15241 cgcttccagt tgctgccagg cagtaaacac ctgctgtgcg gcctgttcag aaatcagccc
    15301 gccaataaac ggctgcggtt cgtcatccca gttgcccggc gttaatcgct ggctgacggc
    15361 aaccagacga gcaagaaatg catcgccctg cgccccgctt ttcagcaata cacggcgggc
    15421 gcaggtgcag cgttgcccgg ctgtaacaaa cgccgactga atggtcagat ggacagccgc
    15481 gtcgatatcc gccacctcat cgataatcag cgggttattg ccgcccatct caagggcgag
    15541 aattttctcc ggctgaccgg agagctggcg atgcagctgg tagccggtat tggcgctgcc
    15601 ggtaaacagc aagccgtcga gatcctccag cgcactcagc gcctgacccg tttcacaccc
    15661 gccctgcacc aggttcagca cgcctggcgg aaagccagcc tgctgccata aacgcattac
    15721 cgcttcgcca ctccacggtg tcagttcgct gggtttaaag ataatggtgt tacctgccag
    15781 caatgcggga acgatatgtc cgttcggcaa atgaccaggg aaattatacg gcccaaacac
    15841 cgccagcacg ccgtgcgggc gatgtcgcag gctcgccgca ccgtccggca tttcactacg
    15901 ctgctcgccg gtacgaacgt gatacgcctt aattgatatc gcgattttat tgatcatcgc
    15961 cgtcaattcg gttgccgcct cccagcgcgg cttgccggtt tctcgggcaa tgattgcggt
    16021 taattcgcct ttattccttt ccagcaatcc ggcaaagcgt tcgacaacgg cctgacgttc
    16081 agcaaatgag agccgcgccc agcgcggaaa cgccgcacgg gctgcccgac aagcctgccc
    16141 gacctgagcg gcatcggcat cattgccttg ccataacacc tcgcccgata ccggattacg
    16201 cttcacacgc gatgcaccct ggcccgttat ccagtcaccg ttaatccata aagtcatgct
    16261 gttttctcct ctgcgcacag gcgcaccaga cgaacgcgat cccctgcatg gcatttgagg
    16321 gcatccagtt gtgcggcggt taaaatcaaa cgctcggttg ccggatcggt acgcaccagc
    16381 accacgcgga aatggtgata attttcattg gcgaccaggc aggccggaaa atcgccctgc
    16441 gcaggctgcc cttctgccac ttccaccagc cgacttttac ggatggcgcg cacgcggtcg
    16501 atgtcacact caagcgtcgg cccaccgtcg aagatgtcga tatagttgcg gtagcgaaaa
    16561 ccttctttct ccagcaccgc gcgggcaggc gcggtttgcg gatgtacctg accgatgacg
    16621 tcctgggctt cctgggataa aaagtgcgta tagatcggat gtttcggcat cagttctgca
    16681 ataaatgcct tttgcccggt gccgcagaga aaatcggcgc ggctaaaatc catcgaaaag
    16741 aagcgtttac cgaggctttg ccagaacggt gaatagccgt gttcgtcaat caccccgcgc
    16801 atttcagcaa ccactttgtc attaaacttg tcgcgaaaag ccgccataaa cataaagcgc
    16861 gatttcgaca gcaaatagcc gttgccctct ttgcgccagt ccgggtcgag aaacagcgtg
    16921 cacagctcgc tgctgccggt gtgatcgtta ctgagaaaca acgtcggcaa tgcgttatag
    16981 acattcagct cttttgaggc gtgaaccaac gtgccgacgc gatagttgta ccagggatcg
    17041 ttcagcccaa ctgccacctc aatggcgcaa atccccgcca cggtgcctgt ctcgctatct
    17101 tccagtacaa acacataacc ctgctcactt ttgggcagtt caccttgcca ggttttgatt
    17161 gccctttcga tacgcgccga aagcgtggct tcattggcgg gaagcgacgt caggccgccg
    17221 cccgttttgc tggcaagctg catcagcgcc gagacatctg agcgctcaac gggacggatg
    17281 accatcatga tgaacctcgg ctaacaaagt gttcgcaagc aactgcaaag cgatccagtc
    17341 cggtcgtcac ctcttcttcg ctgacattga gcgcaggcgc aaaacgcacc acgttgccac
    17401 ccgcaatcag taccatcacg cctgctttcg ccgcttcctg agagatctgt ttcgcttgcc
    17461 cggcgtaatc ggcattcagt acacagccaa tcagcaaacc taagccgcga acttcattga
    17521 acaaaccata gcggtgatta ataatattaa gacgctcaac aaaccagtcg tgacgttgct
    17581 taaccccgtt aagcatctct ggcgtattga tgagctccag cactttgcct gccaccgccg
    17641 aggccagcgg attgccgcca taggtggtgc catgagtgcc aacggtcatc acgctggcgc
    17701 actcttcggt tgctaacaac gcaccgaccg ggaaaccgcc gcccagagct ttggcggtag
    17761 ttaacagatc aggcgtcacg ccgtagtgca tataggcata cagttccccg gtacgcccga
    17821 cgccggtttg tacttcatca aaaatcaaca gcgcattgtg gcgatcacac aattcacgca
    17881 gaccttgtaa aaatgcgttg ctggctggca ccacaccgcc ttcaccctgg atgggttcga
    17941 caatcaccgc acaggtagag tcgtcaatca gcgcgctggc agagttaata tcgttatatg
    18001 cagcatgacg gatatctggt ggcagtggcg caaaatcctg tgaataggct ggctgcccac
    18061 ccgcactaac agtaaacagc gtgcgaccat gaaacgcatt tttaaacgcc acgataccgc
    18121 tcttatggct gccgtagcgg tcgtgagcga atttacgcgc cagttttagc gccgcttcgt
    18181 tggcttccgc accggagtta caaaagaaaa cgcgatcggc aaacgtggcg tcgatcaatt
    18241 ttttcgccag tcgcagtacc gactcgttgg tgtaaccgtt gccggtatgc cagaacttac
    18301 tcgcctgttc gttcagcgcc tcacgcagtt ccggatgcgc atggcccagc gcgttcaccg
    18361 caatgccacc cgcgaagtcg atatactctt tcccctgctg atcccacaag cgcgaacctt
    18421 cgccacgtac cggtataaac ggtgccggag cgtaaacagg tatcatccat tcatcaaagt
    18481 tttcacgcgt aattggctga gacatagcga cctctacagt aaatagctcg ttatttatat
    18541 gttaataata agtaatgttt tcgctgtaaa tgtagattgc agggttcgtg ccagccagtg
    18601 atgaaagtgc ataaacggcg gaggctaact ggaaatcaag gagttataac caaaccatat
    18661 gcatttaaag tgcatattaa gtgaatacgt ttgcgatgtg ggtgaataaa aagaataaaa
    18721 aacgcaatgt tatgcagaag taaaatataa ttctggaatt gtgatcattg acgaaattta
    18781 ctggaaatta ctgcgccatt ctgacgcagc gcgcaccaaa agcgggcatt ttttgcgcca
    18841 tcgttgacat cattaacaac catcgatcaa atcacttaac aacaggcggt aagcaaagcg
    18901 aaattctgct accatccacg cactctttat ctgaataaat ggcagcgact atgaaatttg
    18961 tctcttttaa tatcaatggc ctgcgcgcca gaccccacca gcttgaagcc atcgtcgaaa
    19021 agcaccaacc ggatgtgatt ggcctgcagg agacaaaagt tcatgacgat atgtttcccc
    19081 tcgaagaggt ggcgaagctc ggctacaacg tgttttatca cgggcagaaa ggccattatg
    19141 gcgtggcgct gctgaccaaa gagacgccga ttgccgtgcg tcgcggcttt cccggtgacg
    19201 aagaagaggc gcagcggcgg attattatgg cggaaatccc ctcgccgctg ggcaatgtca
    19261 ccgtgatcaa cggttacttc ccgcagggtg aaagccgcga ccatccgata aaattcccgg
    19321 caaaggcgca gttttatcag aatctgcaaa actacctgga aaccgaactc aagcgtgaaa
    19381 atccggtgct gattatgggc gatatgaata tcagtcctgg cgacctggat attggcattg
    19441 gcgaagagaa ccgtaagcgc tggctgcgta ccggaaaatg ttctttcctt ccggaggaac
    19501 gcgaatggat ggagagactg atgagctggg gattggttga taccttccgc catgcgaatc
    19561 cgcaaacagc agatcgtttc tcatggtttg attaccgctc aaaaggtttt gacgataacc
    19621 gtggtctgcg catcgacctg ctgctcgcca gccaaccgct ggcagaatgt tgcgtagaaa
    19681 ccggcatcga ctatgaaatc cgcagcatgg aaaaaccgtc cgatcacgcc cccgtctggg
    19741 cgaccttccg ccgctaattt agcagctctc ctggctcaaa ctgggtcagg agaattaacc
    19801 ttgagaaaaa tcaacaaact gtcagtaatg atttgttgcc tgccgtcctt tgttataccg
    19861 tcactgcgtt tttagttgtc tgaccacttc tctattatca agtttgatat aggaaactcc
    19921 acgatgaacg ctgagcgtaa atttcttttt gcctgtctta tttttgcgct ggccatttac
    19981 gctatccacg ctttcggttt attcgatctg ctcaccgatt taccccactt acagacactc
    20041 attcgccaga gcggattgtt cggttatagc ctctatattc tgttattcat cattgccacc
    20101 ctctttctgt taccaggaag catattggtg atcgcgggag gaatagtttt tggcccgttc
    20161 ttagggacac tactctcatt aattgccgcc acgctggcct cctcgtgctc attcctgctg
    20221 gcgcgctgga tgggacgtga tttactgctg aaatacgttg gccatagcca taccttccag
    20281 gccattgaaa aaggcattgc gcgtaacggt attgattttc ttattctgac ccgcttaatc
    20341 ccgttgtttc cttacaatat tcaaaattac gcttacggat taaccacaat cgccttctgg
    20401 ccttataccc ttatttcggc actcacgacc ctgcccggta ttgttattta taccgtgatg
    20461 gcaagcgatc tcgccaatga aggcattacg ctgcgcttta ttttacaact ctgtctggcg
    20521 ggcctggcgc tgtttattct cgtccagctc gcaaaactct acgcccgaca caaacatgtg
    20581 gatctgtctg cttcgcgccg caacccactt actcacccaa aaaatgaagg atagaacgat
    20641 gttgcaacat tattcagtgt catggaaaaa aggactgact gcactctgct tactggctgt
    20701 tgctgggctt agcggctgcg atcaaaaaga gaatgcggcg gcaaaagtgg aatacgacgg
    20761 actttcgaac agccaaccgc tgcgcgtcga tgccaataac catacggtaa ccatgctggt
    20821 gcaaattaat ggtcgtttcc tcaccgacga cactcgtcac ggtattgtgt ttaaagatgg
    20881 ctccaacgga cataaatcgc tgtttatggg ttatgcgacc ccgaaagcat tttatgaagc
    20941 cctgaaagag gcaggtggta caccgggcga aaacatgacg atggataata aagaaacgac
    21001 tcatgtcaca ggcagcaaac tggatatttc ggtcaactgg caaggggcgg caaaaacgta
    21061 ttccttcgat gaagtgattg ttgacagtaa tggcaagaaa ctggacatgc gctttggcgg
    21121 taatttaacg gcagcagaag agaagaaaac gggttgcctg gtgtgtctgg atagctgccc
    21181 ggtcggcatc gtcagcaatg caacatacac ttatggtgcg gttgaaaaac gaggtgaagt
    21241 taaattcaaa ggcaatgcct cggttctccc ggcgaataac acgctggcaa cggttacctt
    21301 taaaatcacc gaataaagcc aggataaagg atgatgaaga tgcaatcgcg taaaatctgg
    21361 tactaccgta taaccctcat catcctgttg ttcgctatgc tgctggcatg ggcgctgctt
    21421 cccggcgtcc atgagtttat caatcgcagc gttgcggcgt ttgccgccgt ggaccaacag
    21481 ggtatagaac gctttattca gtcttacggc gcactggcgg cagttgtctc gttcttgttg
    21541 atgattttac aggccattgc tgcaccgcta cctgcgtttt tgatcacctt tgccaatgcg
    21601 tcgctgtttg gcgcgttctg gggcggctcg ctgtcgtgga ccagttcgat ggccggcgcg
    21661 gcactgtgct tttttatcgc cagagtgatg ggccgcgaag tggtggaaaa attaaccggc
    21721 aaaaccgtac ttgacagtat ggacggcttt ttcactcgct acggcaaaca caccatcctg
    21781 gtctgtcggt tattgccttt tgtccctttc gatccaatca gctatgctgc cggtttgact
    21841 tcaatacgtt ttcgctcgtt ttttatcgcc accgggcttg gtcagttacc ggcgactatt
    21901 gtttattcct gggcgggcag catgttaaca ggcggtacct tctggtttgt caccggactg
    21961 tttattctgt ttgccctgac cgtggtgatt tttatggcga agaaaatatg gcttgagcgc
    22021 cagaagagga atgcctgatg ggtttaccgc cgcttagcaa aattcctttc attttacgtc
    22081 cgcaggcgtg gctgcatcgt cgccattacg gcgaggtgct aagccccatt cgctggtggg
    22141 ggcggatccc gtttatcttt tatctggtgt cgatgtttgt tggctggctg gagcgcaaac
    22201 gctcaccgct cgatccggta gtacgatcgc ttgtcagcgc gcgcattgcg caaatgtgcc
    22261 tgtgtgagtt ttgtgtggat atcaccagta tgaaagtcgc cgagcgcacc ggcagcagcg
    22321 ataaactgct ggcagtggct gactggcggc aaagcccgct ctttagcgat gaagaacggc
    22381 tggcgctgga gtacgccgaa gccgcaagcg taacgccgcc aacggtcgat gatgccctgc
    22441 gtacccgact ggctgcgcat tttgacgctc aggagctcac cgaactgacg gcattgatcg
    22501 gcctgcaaaa tctgtcagcc cgttttaatt ctgccatgga cattcccgct caggggctgt
    22561 gccgtattcc ggaaaaacgt tcttaaggag agatgatgcg ccattgtggg tggttgctgg
    22621 gattgttatc gctgttttct ctggcaacac atgccagtga ctggcaagaa attaaaaatg
    22681 aggccaaagg gcaaactgtc tggtttaacg cctggggcgg cgataccgca attaaccgct
    22741 atctcgactg ggttagcggc gagatgaaaa cccattacgc tataaacctg aagattgttc
    22801 gtctggcgga tgccgcagac gcggtgaagc gcattcagac cgaagctgct gccggacgta
    22861 aaacgggcgg ctcggtggat ctgctctggg tgaacggcga aaacttccgc accttaaaag
    22921 aggccaattt actgcaaacc gactgggcag agactctgcc caactggcgc tatgtcgaca
    22981 cacagctgcc ggtgcgggaa gatttttcag tgcctacaga aggggctgaa tcgccctggg
    23041 ggggcgcaca actgacattt atcgcccgcc gcgatgttac gccacagcca ccacaaacgc
    23101 cgcaagcctt actggagttt gctaaagcca atcccggcac ggttacctat ccgcgcccac
    23161 cggactttac cggcacggcg tttcttgaac agttgctgat tatgctgacg cccgatcccg
    23221 ccgcattaaa tgaagcgccg aacgatgcga ctttcgcccg tgtcactgct cccttgtggc
    23281 aatatcttga tgcgctgcat ccgtatttgt ggcgcgaagg aaaggatttc ccgccttcgc
    23341 ccgcgcggat ggatgctctg ctgaaagccg gaacatttcg cctgtcgctg acctttaacc
    23401 ccgcgcatgc gcagcaaaaa atcgccagcg gcgatttgcc tgcaagcagt tacagttttg
    23461 gctttcgcga ggggatgatt ggcaacgtgc atttcgtcac cattcctgcc aacgcgaatg
    23521 ccagtgctgc ggcgaaggta gttgccaatt tcttgctctc acccgatgcg caattgcgta
    23581 aagcagatcc cgctgtctgg ggcgatcctt ctgttctcga tccgcaaaaa ctgcctgatg
    23641 ggcagcgcga aacattgcaa tcaagaatgc cgcaagatct gccgccggta ctggctgaac
    23701 cgcacgcagg ttgggtaaat gcgctggaac aagaatggct acaccgttac ggtacgcatt
    23761 aatctttttg ctgtgggcga tggtggcggt gatttatgca ccgctgatcc cggcagctct
    23821 cacgctgata tcgcctgctt tgtcgttgac acactggcag gcgttatttg ccgatccaca
    23881 gttaccgcag gcattactgg caacgctggt gtcgacaacc atcgcggcgg tcggggcgtt
    23941 gttgattgcc ctactggtga ttgtggcgct gtggcctggg ccgaaatggc agcgtatgtg
    24001 cacccgtctg ccgtggctgc tcgccattcc ccatgtggct tttgccacca gcgcccttct
    24061 gctctttgct gacggagggc tgctttatga ctatttcccg tatttcactc cgccaatgga
    24121 caaattgggt attgggctgg gcttcacgct ggcggtgaaa gaaagcgcat ttctgctgtg
    24181 gatcttagca gcggtattga gtgaaaaacg gcttttacag caagtcatcg tgctggattc
    24241 gctgggctac agccgctggc aatgcctgaa ctggctgctg ttgccctccg tcgcgcctgc
    24301 gctggcaatg gcgatgctgg cgattgttgc ctggtcgctg tcggtcgtag atgtggcgat
    24361 tattcttggg ccaggtaatc caccgacgct ggcagtaatt agctggcagt ggttaactca
    24421 gggcgacgcc gatcaacaaa cgaaaggcgc actcgccagc ctgctgctga tgttgttact
    24481 cgccgcctac gttttgctgg gctatctgct atggtgtacc tggcggcgta ctattccccg
    24541 cgtagatggc gttcgtaagc ctgccacgcc tttattgcca ggcattacgc tggcgagttt
    24601 tttacccttg accagtgtgc tgtgtgtggt tctgctggcg atcctcgcgg atcagtcgac
    24661 gatcaatagt gaagcgctca tcaacagcct gacaatgggg ctggtggcga cattcatcgc
    24721 tttgctcctg ttactgctgt ggctggaatg ggggccacag cgtcgccagt tgtggctatg
    24781 gttgccgatt ttattaccgg cactgccgct ggtggcgggc cagtacacgc tggcgctatg
    24841 gctgaacatg gatggaagct ggacggcggt ggtctggggg catctgctgt gggtaatgcc
    24901 gtggatgctg tttatcctgc aacccgcctg gcagcgcatt gattcacggt taattttgat
    24961 tgcacaaaca ctgggctggt cgcgggccaa aatcttcttt tacgtgaaat gtccgcttat
    25021 gctacgccct acgctgattg ccttcgcggt gggattttca gtcagtattg cgcagtatat
    25081 gccgacgctg tggctgggcg cggggcgttt tccgacgctc accactgagg cggtggcatt
    25141 aagcagcggc ggcagcaacg gtattctcgc cgccctggct ttatggcaac tgctattacc
    25201 gcttattatt tttgccctga ccgcgttagt cgcaaaatgg gtaggttatg tcagacaagg
    25261 actccgctaa tgctctgcgt gaaaaatgtt tcgctacgtt taccagaaag ttgcttgctg
    25321 acaaacgtta actttacggt ggataaaggt gacattgtca cgttaatggg gccgtctggc
    25381 tgtggaaaat ccactctgtt ttcatggatg attggtgcac tggccggaca gttttcttgt
    25441 acaggtgagc tatggctcaa tgagcaacgg attgacatgc tacccaccgc acagcgtcag
    25501 attggcattc tttttcagga tgcactgtta tttgaccagt tcagtgtcgg gcaaaattta
    25561 ctgctggcgc taccggcgac acttaagggg aatgcccgac gtaatgccgt gaatgatgca
    25621 cttgagcgtt caggccttga cggagctttc catcaggatc ctgctacttt gtctggcggt
    25681 cagcgagcgc gcgttgctct gcttcgcgcc cttctcgctc agccaaaagc gttgctactg
    25741 gatgagccat tcagccgtct tgatgtggct ctgcgcgata attttcgcca gtgggtgttc
    25801 agcgaagttc gcgaactggc gatccccgtc gttcaggtaa cgcacgatct ccaggatgtt
    25861 cctgctgata gttctgttct ggatatggcg cagtggtcag aaaattacaa caaactgcga
    25921 taacgcaaag tttttctcaa tgcgtcagtt cagaatggcg cactcaaaac tacaatgtcg
    25981 ggattttcga tgaaacgtgt ttctcaaatg accgcgctgg caatggcttt agggctggct
    26041 tgcgcttctt cgtgggccgc tgaactggcg aagcctctta cacttgacca gcttcaacaa
    26101 caaaatggca aagcgataga tactcgctcc agcgcgtttt ataacggctg gccacaaacc
    26161 ttaaatggcc cttctggtca tgaacctgcc gccttaaacc tctctgctag ctggcttgac
    26221 aaaatgagca ccgaacagct caacgcgtgg atcaagcaac ataacctgaa agccgatgcc
    26281 ccggtggcgc tgtacggtaa tggcaaagat gtcgacgccg tcaaaacgcg actgcaaaaa
    26341 gcaggtttta cgcatatctc cattctgagt gacgcgctaa gcgaaccttc ccgtctacaa
    26401 aaactgccgc actttgagca gctggtttat ccgcaatggc tgcacgacct gcaacaaggt
    26461 aaagaggtta cggcgaaacc tgccggtgac tggaaagtca ttgaagcggc caggggcgct
    26521 cctaagcttt accttatcag ccatattccc ggtgctgact acatcgatac caacgaagtg
    26581 gaaagcgaac cgctgtggaa caaagtttct gatgaacaac taaaagcgat gctggcaaaa
    26641 cacggcattc gccatgacac cacggtcatt ctgtacgggc gtgacgtata cgctgcagcg
    26701 cgtgtggcgc agattatgct ttatgccggc gtgaaagatg tgcgcctgct ggatggcggc
    26761 tggcaaacct ggtccgacgc gggtctgccc gttgagcgcg gtacgccacc gaaagtgaaa
    26821 gcggaaccgg atttcggcgt gaagatcccg gcacaaccgc agttgatgct cgatatggaa
    26881 caggcccgtg gactgctgca tcgccaggat gcatcgctgg tgagcattcg ttcgtggcca
    26941 gaatttatcg gtacgaccag cggttacagc tatattaaac caaaaggtga aatagccgga
    27001 gcacgttggg gacacgctgg tagcgactcg acgcatatgg aagatttcca taatccggat
    27061 ggcaccatgc gtagcgccga tgatattacc gctatgtgga aagcatggaa tatcaaacca
    27121 gatcagcaag tttcattcta ctgcggcacc ggctggcgcg cgtccgaaac ctttatgtac
    27181 gcacgagcaa tgggctggaa taacgtctcc gtttacgacg gcggctggta cgaatggagc
    27241 agcgatccaa aaaatccggt agcaaccggt gaacgcggcc cggacagcag caaataacat
    27301 tgcaattact gacgctggag cgacttcagc gtcagatacc cactccacac ccgcgtaaag
    27361 gtcgtcatcc agcacaacgc accgaagatc cacgcaaacc acgcaaacca tgcagggaat
    27421 aaacatccca gcacaaacag taagatcgtt tctgtgcctt cggttaatcc ccccagataa
    27481 taaaacgatt tatgcgcata accgggatta tcaatctgat atttcgccgc cagcgcagca
    27541 aaagcgagaa aactgctacc cgtgccgata aacgcaaaca gcagccagcc gcctgccagg
    27601 gcgttttgct gcggtgcggc gaggataaac ccaaaaggta ccagcgcgta aaaaagaaaa
    27661 tcgagagaaa tatcaagaaa gccgcccgcg tcggtaagcc ctctgcgccg cgccagcgca
    27721 ccgtccagac catcaagcag cctgttcaac aaaatgacga caagcgccgc cagataccag
    27781 cccagcgcca gaaaaggcag tgccagcaca ccgatggcaa atcccactaa tgttaaacca
    27841 tctggcgtaa tgcccggttt atcaagaacc cgcacacact gatgcaacaa cggtttaatc
    27901 cggggatgaa gatggcggtc tagcacaggc actccttaaa tataaagcct ttctgattga
    27961 gcaacagtgc ggatattatg gcatttttcg cttatctgcc cgtgtgtaat ttatgaaaat
    28021 gattgaagtt gttgccgcca tcattgaacg tgatggcaaa attttactcg cgcaacgccc
    28081 cgcccagagc gatcaggcgg gattatggga gtttgccggt ggtaaagtcg agccggatga
    28141 aagccagcag caggcgctgg tgcgtgagtt aaacgaagaa ttgggcatcg aagcaactgt
    28201 gggtgagtat gttgccagcc atcagcgaga agtttcgggc cggattatcc atcttcatgc
    28261 ctggcacgta cccgacttcc acgggacgtt acaggcacat gaacatcagg cgctggtctg
    28321 gtgctcacct gaagaggcgc tgcaatatcc gctggctcct gctgacattc cattattaga
    28381 ggcgtttatg gctttacgcg ccgccagacc agcggattag tgctaagggt tttgtcatcc
    28441 cgctggcatt gcagcagtat tccttcggct ttaattaccg ccccttcaga ataattttta
    28501 tcctgataaa cgcagcactg agtacagggc tgcgctgact gcccgcctga actgaccact
    28561 tctggcggta cgtttacctc cacgtccgga ccataatgcg ggttagccag tgcaattaat
    28621 ggaaatgcta atactacggc gaacaatgct cgactcacag ggaactcctt aacgttattg
    28681 tctctgctac tgataacggt agccgggtgg caaaacttta gcgtctgagt tatcgcattt
    28741 ggttatgaga ttactctcgt tattaatttg ctttcctggg tcattttttt cttgcttacc
    28801 gtcacattct tgatggtata gtcgaaaact gcaaaagcac atgacataaa caacataagc
    28861 acaatcgtat taatatataa gggttttata tctatggatc agacatattc tctggagtca
    28921 ttcctcaacc atgtccaaaa gcgcgacccg aatcaaaccg agttcgcgca agccgttcgt
    28981 gaagtaatga ccacactctg gccttttctt gaacaaaatc caaaatatcg ccagatgtca
    29041 ttactggagc gtctggttga accggagcgc gtgatccagt ttcgcgtggt atgggttgat
    29101 gatcgcaatc aggtacaggt caaccgtgca tggcgtgtgc agttcagctc tgccatcggc
    29161 ccgtacaaag gcggtatgcg cttccatccg tcagttaacc tttccattct taaattcctc
    29221 ggcttcgaac aaaccttcaa aaatgccctg actactctgc cgatgggcgg tggtaaaggc
    29281 ggcagcgatt tcgacccgaa agggaaaagc gaaggcgaag tgatgcgttt ctgccaggcg
    29341 ctgatgactg aactgtatcg ccacctgggc gcggataccg acgttccggc aggtgatatc
    29401 ggggttggtg gtcgtgaagt cggctttatg gcggggatga tgaaaaagct ctccaacaat
    29461 accgcctgcg tcttcaccgg taagggcctt tcatttggcg gcagtcttat tcgcccggaa
    29521 gctaccggct acggtctcgt ttatttcaca gaagcaatgc taaaacgcca cggtatgggt
    29581 tttgaaggca tgcgcgtttc cgtttctggc tccggcaacg tcgcccagta cgctatcgaa
    29641 aaagcgatgg aatttggtgc tcgtgtgatc actgcgtcag actccagcgg cactgtagtt
    29701 gatgaaagcg gattcacgaa agagaaactg gcacgtctta tcgaaatcaa atccagccgc
    29761 gatggtcgag tggcagatta cgccaaagaa tttggtctgg tctatctcga aggccaacag
    29821 ccgtggtctg taccggttga tatcgccctg ccttgcgcta cccagaacga actggatgtt
    29881 gacgccgcgc atcagcttat cgctaatggc gttaaagccg tcgccgaagg ggcaaatatg
    29941 ccgaccacca tcgaagcgac tgaactgttc cagcaggcag gcgtactatt tgcaccgggt
    30001 aaagcggcta atgctggtgg cgtcgctaca tcgggcctgg aaatggcaca aaacgctgcg
    30061 cgcctgggct ggaaagccga gaaagttgac gcacgtttgc atcacatcat gctggatatc
    30121 caccatgcct gtgttgagca tggtggtgaa ggtgagcaaa ccaactacgt gcagggcgcg
    30181 aacattgccg gttttgtgaa ggttgccgat gcgatgctgg cgcagggtgt gatttaagtt
    30241 gtaaatgcct gatggcgcta cgcttatcag gcctacaaat gggcacaatt cattgcagtt
    30301 acgctctaat gtaggccggg caagcgcagc gcccccggca aaatttcagg cgtttatgag
    30361 tatttaacgg atgatgctcc ccagggaaca tttcttatgg gccaacggca tttcttactg
    30421 tagtgctccc aaaactgctt gtcgtaacga taacaagctt caagttcagc atccgttaac
    30481 tttctgcgat agcagcagat atgccagtaa agaaatccca ttagactatt tttttgataa
    30541 tcttcttcgc tttcgaacaa ctcgtgcgcc tttcgagaag caagcattat ataatgccag
    30601 gccagttctt cttcaattgt cccgttttga aaagctgtgc ttgatatcga gatcatccat
    30661 gataattccg ccgcccatat tagcttcgcc gaggatttac cggagctatg attagcgcaa
    30721 tcagagatat agtctgaggg aaaaacagca aatttattca acaaggcgat aacctgctct
    30781 ggggcttcct ccatgtttgc tttaaaggta ttggctccat ggtcgccaga aagaaaatgc
    30841 tccattaagg cacaataact ttcgctatct tcgatacccc attgttcctc taaagactcg
    30901 cgtcttttac ttatgatatc gatcgagtca aaaggaagca catgatattg gaaggtatct
    30961 ttgccaggtt caggctttcg cggccagaac tccagcgttt cagaccattg cttatgatag
    31021 aatcgataag gtgcgatcaa ttgtagcgcc tgtaatttct cgatactgag cggctcaata
    31081 cctttagcct gataataatg cagctgttct ttttttgctt taaaaccggc ccgaacaata
    31141 agccccatca taattaatag ataaagaaaa gagcatcccg cggtaatcag gcctctttca
    31201 ttcaaaccgt tggatgttat cgctgcgaat acaaacatta cagcgacaac acatgttaaa
    31261 taaaaccccc acttacaaag cagcatggtc ttattttctt taatcatccg ttcaaaatta
    31321 ctattaaata tttcccagcc attaaaagaa tacttctcgc tcccaggatg gttttgtaat
    31381 aaaacttttt tcatcacatt ccctgttatt acatacttta gaggcgataa ttttatccat
    31441 gcaaaaaaaa tatccaacga attttcttga tccgctggat atttcagaat atgactcgaa
    31501 tagcacgaaa gattcgctcg cttacgctat cgccccgctt ccggcttcat ctgctggcgg
    31561 actttttttc gcactacgtt tacgcggtgc agcctttttc ttatcagcac tgccaccact
    31621 gcccggagcc acaatgccgc gaaactgccg caccggagta cgtttggctt gatcaataag
    31681 ctgatatagc gtccccacca gcggctgcat aaagtcctga tagcgacact gcttttcgct
    31741 gatttgcgtc agcaccgatt cccagtgcgc ggtcatgtcc ggtcgcgtag ccatttctgg
    31801 cagcgaatgg aataacgctt ttccggcgtc ggtggagtgg atatagcgcc cttttttgat
    31861 gaggaaacca cgcttgaaca acagttcaat aatcccggca cgcgttgctt ctgtccccag
    31921 accatcggtc gcacgaagga tctttttcag atctttgtcc tgcacaaagc gcgcgatccc
    31981 ggtcatcgcc gaaagcagtg ttgcatcggt aaaatggcgc ggcggctggg tttgccgctc
    32041 taccacttca cctttttcac acagcaactc atcgcctttc gccaccacag gcaatggcgt
    32101 accgtcgttt tcttcatcgc gctctttgct gcctaacagc gtgcgccagc ctgcttcagc
    32161 aagaaaacgc gctttagcga caaatttgcc tttggcaatg tccagttcga taacacactt
    32221 gcggaacacc gcatccgggc agaactgcat cagatactga cgggaaatca ggttatagac
    32281 cttcgcttcg ttctccgtca ggttgatcgc agaactccgt gcggtcggaa tgatggcgtg
    32341 gtgcgcatcg acctttttgt catcccaaca gcggttgcgt atatctggat ctaccactgg
    32401 ctgcggcaac agatctggtg catgaacgct gatggcattc atcaccgcgt ggcgtccggc
    32461 aaaatgttct tctggcaaat agcgacaatc ggaacgcgga taagtgatta gcttgtgcgt
    32521 ttcgtacagc ttctggcaga tatcaagcac gttctgcgcg ctcagaccaa agcgttttgc
    32581 cgcttcaatc tgcaacgccg aaagcgaaaa cggcagcggc gcggattctg attcccgttt
    32641 atcgttatag ctggtgacaa tagccggttg accactaatg cggttaacca catgctccgc
    32701 cagtggacga tgtaacaagc gcccttcttc atcctggtac ggttcacacg cttcgctcgg
    32761 ttgccagata gcggtaaacc gctcatcggc aggtgtcacg atatgtgctt tgacttcaaa
    32821 gaagtctttc gccacgaagt tttcaatctc ttcatcgcgg cgcaccacca gcccaagcac
    32881 gggcgtctgc acgcgtccca cggaaagcac gccctgataa ccggcattgc gaccgagaat
    32941 ggtatacgca cgggtcatat tgatgccgta cagccagtcg gcacgcgctc gcgccagcgc
    33001 agaaacgcac agcggtacaa actcactgtt ggaacgaaga cggtcgatcg cccgctcaac
    33061 cgcctgcggg ttcaggtcgt ttatcaagca acgctgcacc tgctggcgct tttccggtgc
    33121 cagttgtaga tagtccagca cttcatccac cagcagttgc ccttcgcgat ccgggtcccc
    33181 ggcgtgaacg atttcgctgg cttcatgcag gtaccgtttg atgacgttaa gttgtttggt
    33241 cacggagggt cggggctgta attgccactt ttccgggaca atcggcaaat ccgcaagatt
    33301 ccagcgcgca tagcggctgt cgtaggcgtc tggctgcgcc tgctcaagca ggtgaccgat
    33361 acaccaggtc accacctgac cattaccgca ctcgataaag ccatcgcctt tccggtgcgg
    33421 tttgggcagg acatcagcaa tggcgcgcgc cagactcggt ttttcggcaa taaacaaccg
    33481 cattgaatta acggatctca accatggcac gaccgccgcg tgctggcacc agttcgccaa
    33541 tcgccgtcag ttcaatgcca aactcggcgg ctgtagcttt gacctcattt tcggcttccg
    33601 gcatgaccgc cagcagcaaa ccgccagaag tttgcggatc gcacagcaaa tcgcgcactt
    33661 cacgcggcat ttcacccatc agatgaccgt agctggcaaa gttacgttcg gtgccgccag
    33721 gcactgcacc caacttaatg tactcttcaa cccccggcaa tttcgggatc gcgtcatagt
    33781 caacgcgcgc ctgcacacct gcgccctgac acatttcgct caaatggccc agcaggccaa
    33841 agcccgttac gtcggtcatc gcttttacgc cttcaatgtt ggcaaaggac gcgcctgcga
    33901 tgttcatccg gcacatcact tccgtcgcca gtccctgatg ttctggtttc agcagtgatt
    33961 ttttctcagc cgtggtaaga acgccgatcc ccagcggttt cgtcaggaac agtttacatc
    34021 cggcttgtgc ggtactgttt ttcttcaccc gctcggtcgg tacgatcccc gttaccgcca
    34081 gaccaaaaat cggctccggc gcatcgatgg agtgaccgcc agccagcgca atacccgcct
    34141 gacggcatgc atagcgtcca ccttcggtta cttcgcgggc aatttccggg gaaagtttgt
    34201 taatcggcca gccgaggatc gcaattgcca taatcggttt gccccccatc gcgaagatat
    34261 cgctgatggc gttagtcgcc gcaatgcggc caaaatcgaa aggattatca acgatcggca
    34321 taaagaagtc ggtggtactg ataacgctgg tgccattgcc cagatcgtac accgccgcat
    34381 cgtcgcgggt ttcattaccc acaagcaaat tcggatcaac aaacttcgcc tgctcactgt
    34441 gcaggatggt ttccaacact tttggggaaa ttttacagcc gcaaccagct ccgtggctgt
    34501 attgggtcaa acgaatcgag ttctcgctca tggacatctc ctgtcaatgc aatccgggta
    34561 tggtaaccct cattccgtga agtgataagt gagagtgtct gaattcctgc gcctttgctc
    34621 acaatccaga cagtttcgcg acaattatca gaaataagtg acaaacggcg tcgggtccgg
    34681 gacgtttatc gacgtagatg ctttcagctg cggcgtgccg aggtagagaa aaccgacaat
    34741 tttatcctgc tcacggcaac cgaatgcttc acgcactacc ggactttcag ttaatgcgcc
    34801 actgcgccag atgccgccaa acccctgggc aacggctgcc atttgcatcg ccatgaccgc
    34861 gcatccggca gacatttcct gttcccatcg cgggacttta tgattttctt cgcatttcgc
    34921 caccaccgtg atgatgagcg gtgcgcggaa cggcgcatta cgggctttgt cgatagcttt
    34981 gtcatcacta ccggcagcaa tcgccccctg ttccagtacg gcgctgaaac gctcgcgccc
    35041 ttccccttca atcacaaaaa aatgccacgg ttgcatggac ttatggtccg gcgcgcgcat
    35101 acccgcacgc aggatgtttt gcagttgttc acccgttggt gcgggttcag ccaggcggga
    35161 ggcgctacgg cgattgatca atagttcgag tgcatccatt tgattaactc ctgtcgtgat
    35221 atttattcac aaaattaaca cgagagtgga ttttgttaca gcacagtccg caattcctgc
    35281 tgacaagtgc cggttgggtc attacgataa ccacatctat tgcgcctgtg acaggtgtga
    35341 ccttaagttg ggagaataca tgcgaaccct ttggcgattt attgccggat tttttaaatg
    35401 gacgtggcgt ctgctgaatt tcgtccgtga aatggtactt aacctgttct ttatttttct
    35461 cgtgctggtt ggtgtgggga tctggatgca ggtcagtggt ggtgattcga aagaaacggc
    35521 cagtcgtggt gcactgctgc tggacatttc tggtgtgatc gtcgataaac ccgacagttc
    35581 tcagcggttt agtaaattaa gccgccaact acttggtgcc agttccgatc gtctgcagga
    35641 aaactcactg tttgatatcg tcaataccat tcgccaggcg aaggatgacc gcaatatcac
    35701 cggtatcgtg atggatctga aaaacttcgc aggcggcgac caaccgtcta tgcagtacat
    35761 cggcaaagcc ctgaaagagt ttcgtgacag cgggaaaccg gtttatgccg ttggcgagaa
    35821 ctacagccag gggcaatatt atctcgccag tttcgccaat aaaatttggc tgtcaccgca
    35881 aggcgtggtg gatttgcacg gttttgctac caacggtctg tactacaaat cgttgctgga
    35941 taagctgaaa gtttccaccc atgtgttccg cgtgggtacg tataaatctg ccgttgaacc
    36001 gtttattcgt gatgacatgt caccggcagc ccgcgaagct gacagccgct ggattggtga
    36061 gctgtggcaa aactatctga atactgttgc cgctaaccgg cagatccctg ctcagcaggt
    36121 attccctggc gcgcaagggt tgcttgaggg tttaaccaaa accggtggcg ataccgcgaa
    36181 atatgcactg gaaaacaagc tggtcgatgc actggcatcg agtgcggaaa tcgaaaaagc
    36241 actgaccaaa gagttcggct ggagtaaggc tgataaaaat tatcgcgcca tcagttatta
    36301 cgattacgca ttgaaaacgc cggcagatac cggtgacagc atcggtgtcg tcttcgctaa
    36361 tggcgcaatt atggatggcg aggaaactca ggggaatgtt ggcggtgata ccactgcggc
    36421 acaaatccgc gacgctcgcc ttgacccgaa agtgaaagcg attgtcctgc gtgttaatag
    36481 cccaggcggc agcgttaccg cgtctgaagt gattcgcgct gaactggcag cagcccgggc
    36541 agcgggtaag cctgtggttg tatcgatggg cggcatggcg gcatctggtg gttactggat
    36601 ttccacgcca gctaattaca ttgtggctaa ccccagcacc ctgaccggtt ctattggtat
    36661 cttcggcgtg atcaccaccg tagaaaatag tctggattcg attggtgttc atactgatgg
    36721 tgtctcaact tcaccgctgg cggatgtttc tatcaccagg gcactgccgc cggaagcgca
    36781 gcagatgatg cagttaagca ttgagaatgg ctataaacgc tttatcacgc tggttgctga
    36841 tgcgcgtcat tcgacgccgg agcagattga taaaatcgcc cagggccacg tctggaccgg
    36901 tcaggacgca aaagctaacg ggctggtcga tagtctcggg gatttcgatg atgcggtcgc
    36961 caaagcagca gagctggcaa aagcgaaaca gtggcatctg gaatactacg ttgatgaacc
    37021 gaccttcttc gacaaagtga tggacaacat gtctggttct gtccgggcaa tgttgccaga
    37081 tgcgttccag gccatgttac ctgcaccgct ggcctcggta gcctctaccg ttaaaagtga
    37141 aagcgacaag ctggcggcgt ttaacgaccc acaaaaccgt tatgcgtttt gcctgacctg
    37201 cgccaacgtg cgttaaatcc tgcaaaaatg tggccgatat gctgtcggcc acactcctga
    37261 atcggactgt ctttcactcc ctctctccac tacattccct tgctcattta gcatctgata
    37321 tggagtcatc agtgaacaag gccccctctt taattgccgc tatcgtcctc ggactgggga
    37381 ttagcgcctg tggttatttt gttggcgatg gtgttaaaca tttaaaaacc aacaaccgtt
    37441 atgtcaatgt gcgcgggctt tctgaaaaag aagttcgcgc agatacggcg gaattaacaa
    37501 ttgcgattaa ctttaagggt aatgttcccg gcgaactgtt cccgaaactg gaagaagcgc
    37561 agaaaaaaat tgttgccgaa ctcaatgctc aggggattaa cgagaaagag ataattctgg
    37621 gccaatggac aagcaagcga acagattcct tttatctgaa agatgatccc acaatgccac
    37681 gctataacgc agacgggtca gtgacgataa aaacccataa tgttgctgct gtcgaaaaag
    37741 tcgtcgcgaa attaaacgaa ctgcaagtcg ctacggatgg cgctatcgct gagagcaaag
    37801 tcgcctatcg ctttaatggt attggcgcac tgcgcgctga gatgattgct gccgccacca
    37861 aagatgcacg caacgccgca ctacaatttg caacggactc aggtagccag gtggggtcaa
    37921 ttagcgatgc ctcacaaggc gtattccaga tctttgccag tggcagtgat gaagatgatc
    37981 ctacggcgat taataaaacg gtacgcgtag tcacgaccgt aacctatgcg ttgcaggatt
    38041 aaccactttt ctccggcggg acactaatcc ctccgtcctg ccggactttt ctcatatcca
    38101 cagagtatcc aggtgcgctc gctgcccctc acgtatatac ttttgctctt tcgatatcat
    38161 tcatatcaat atcatgcaaa agaaatcaat ttacgttgcc tacacgggcg ggaccatcgg
    38221 gatgcagcgt tccgagcagg gttatatacc ggtgtcaggt catctacaac gccaactggc
    38281 gctgatgccg gaattccatc gcccggagat gccagatttc accattcatg aatatacgcc
    38341 gctgatggat tcttcagata tgacgccgga agactggcag catattgctg aagatattaa
    38401 agcgcactat gacgactatg atggttttgt cattctgcac ggcaccgaca cgatggcgta
    38461 taccgcctct gcgctgtcgt tcatgctcga aaatctcggt aaaccggtca ttgtgacagg
    38521 gtcacaaatc ccgctggctg agttacgctc tgacggacaa attaatctgc tgaatgcgtt
    38581 gtacgttgcg gcgaattatc cgatcaacga agtaacactc ttttttaata accgactgta
    38641 tcgcggcaac cgcactacca aagcccatgc cgatggtttt gatgcgtttg cctctccaaa
    38701 ccttcctccg ttactggaag caggtatcca tattcgtcgt ttgaatacgc cacccgcccc
    38761 gcacggtgaa ggggaattga tcgtacatcc aatcacccca caaccaattg gcgtagtgac
    38821 gatttatcca gggatttctg ctgacgtcgt gcgcaatttt ctgcgccaac cggtgaaagc
    38881 attgattctg cgctcctatg gcgtgggtaa tgcgccacaa aacaaagcct tcctgcagga
    38941 attacaagaa gccagcgatc gcggtattgt ggtggtcaac ctgacacaat gtatgtccgg
    39001 taaagtgaat atgggtggtt atgccaccgg taacgccctc gcccatgctg gcgttattgg
    39061 cggtgcagat atgactgtag aagccacgct aaccaaactg cattacctgc ttagccagga
    39121 actggatact gaaaccattc gcaaggccat gagccaaaac ctgcgcggcg aactgacgcc
    39181 ggatgattaa ggagactgca atgccccctc gcgccctgtt actggtcgat ttacaaaatg
    39241 atttctgtgc tggtggcgcg ctcgccgtgc cggaaggtga cagtacggtg gatgtcgcta
    39301 accgcctgat tgactggtgc cagtcgcgcg gtgaagcggt tatcgccagt caggactggc
    39361 acccggcgaa tcacggcagt tttgccagcc agcacggtgt agagccttat acgccaggcc
    39421 aactcgacgg tttgccacaa accttctggc cagatcactg tgtgcagaac agtgaaggcg
    39481 cacaattaca tccgttactg aaccaaaaag cgatcgcagc ggtgttccat aaaggcgaaa
    39541 atcctttagt tgacagttac agtgcctttt ttgataacgg ccgtcggcag aaaacctctc
    39601 tcgatgactg gttacgcgat catgaaatcg atgaattgat cgttatgggc ctggctactg
    39661 actattgcgt gaagtttacc gtgctggacg cgttacagtt aggttataag gtaaacgtga
    39721 ttaccgatgg ttgtcgtggc gtgaatatcc agccccagga cagtgcgcac gcgtttatgg
    39781 agatgtcagc agctggggca acgctatata cgctggcaga ctgggaagag acgcaggggt
    39841 aattttacgc tggcctacaa ttctgtactg gcattgtagg ccaaataaaa cacgtcagtg
    39901 gcacatctgg caattgatgc catcaacgaa agatttaatt tacctcagaa atctcttcca
    39961 acgacacctt ccgcgtttcg atgccaaaaa tggagagaac cagcgcacag agcaataaca
    40021 tgacaccaag taccataaac accgtgatcg acccataatg tgttaataat gccgcaacgc
    40081 catagggcgt gaagactgcg acgatccgtc cgacggcatt aacgaaaccc gaaccgcgca
    40141 ggcgtaaatg cgttggccaa agctccggga tataaaccgc cgacgcgaag caaacataca
    40201 tgtataaaaa gaagatcatc accaggccat agattaaaat cgcccactct gtagtctgaa
    40261 ttgaatagat atagcctaac actgcaataa taataagtaa ggtggagcca aataaccgac
    40321 gaggaaaatg atcaataatt aatgccgcaa taaatattcc taccggagcg ccaatcataa
    40381 taacagcggt cattaatatt gatttatcga catcaatgcc ggagttaaca aatatggtcg
    40441 gtatccatac ggtgatggta taaagcgaaa tgttcattgc aattaaaaca gtaatcgcga
    40501 ctaatgtacg tcgtaacatt tcacctttaa acaggagcca gaaagtaccc ttgattactt
    40561 taaccttgct gttgctctga tacgaagtaa gcgggggtaa acgaatactc ttctctcttt
    40621 caatttgctg ctctacttca cgaagttggc attctgcacc tgcgatttgc cctttccctg
    40681 ccagccatcg tggcgactca ataaagtatt tacctgagag aaaccaggct aacagtatgc
    40741 caataccacc caacagaaac attatccgcc aactaaaaaa agcgataacc accacaccta
    40801 tcgccgcaga cagcatgggc gaccagttac caacaaatga gagccgcgcg gaccattttc
    40861 cacgcaccgt cgcggggata aactccgtaa atgaggcata gccaaccata atcagcgccc
    40921 ccattcctgt tcccatcagg aagcgaaaga agataagcca gtacatatca gggacaaaag
    40981 cggcccctgt tgcagcaata ccgacgatga gaagatttat gcgaaacgcc ctgcgccgcc
    41041 caaagtaatc accgatgaac ccaccggtaa gagagccgat gaaataacca aacattaatg
    41101 ccgaggtaaa ggcggcattg aggaaattat ttgaccatcc attgcttacc agctttgcta
    41161 agacgacatt accggagtaa ctcaaaaacc c
//
LOCUS       DAZKIP010000025        40802 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZKIP010000025 DAZKIP010000000
VERSION     DAZKIP010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40802
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.025"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..755
                     /gene="espX2"
                     /locus_tag="ACHOUK_001626"
     CDS             <1..755
                     /gene="espX2"
                     /locus_tag="ACHOUK_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspX2"
                     /protein_id="HFP9897098.1"
                     /translation="MRLSHQALISMLEPEVKKKTIWNNYSIYPSLQDTHEVVRDDPET
                     ICMRAFPLFAKGWEYAQKNKKHQLILNALGFKGYIRDIFMSAIMRKTDFVLECNNQPT
                     ELNSSFSSLMNDSDQWQQHTLKDKHYANLLTMLDLNDASESDKSKIFFCLSAVFANIS
                     HSNVFNGIPDASKTLKRYAFALLAKAHSLDESMISNQTFNTYKTVLLDFNNLSNEEAN
                     QLRISSLYRDMVRYAQYRFSKVLSEWTPDAWL"
     gene            1039..3189
                     /gene="dinG"
                     /locus_tag="ACHOUK_001627"
     CDS             1039..3189
                     /gene="dinG"
                     /locus_tag="ACHOUK_001627"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFP9897099.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDMLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            3217..4179
                     /gene="ybiB"
                     /locus_tag="ACHOUK_001628"
     CDS             3217..4179
                     /gene="ybiB"
                     /locus_tag="ACHOUK_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFP9897100.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREEMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WIQRCLAGREPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            4320..5405
                     /gene="hcxB"
                     /locus_tag="ACHOUK_001629"
     CDS             4320..5405
                     /gene="hcxB"
                     /locus_tag="ACHOUK_001629"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFP9897101.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDDFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            5472..5531
                     /gene="ybiE"
                     /locus_tag="ACHOUK_001630"
     CDS             5472..5531
                     /gene="ybiE"
                     /locus_tag="ACHOUK_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFP9897102.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(5634..5894)
                     /gene="ybiJ"
                     /locus_tag="ACHOUK_001631"
     CDS             complement(5634..5894)
                     /gene="ybiJ"
                     /locus_tag="ACHOUK_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFP9897103.1"
                     /translation="MKTINTVVAAMALSALSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(6159..6425)
                     /gene="ybiI"
                     /locus_tag="ACHOUK_001632"
     CDS             complement(6159..6425)
                     /gene="ybiI"
                     /locus_tag="ACHOUK_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFP9897104.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKESQLR"
     gene            complement(6499..7185)
                     /gene="ybiX"
                     /locus_tag="ACHOUK_001633"
     CDS             complement(6499..7185)
                     /gene="ybiX"
                     /locus_tag="ACHOUK_001633"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFP9897105.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFIWIQSMIRDDKKRAMLFELDKNIQNIQSLKSRYGENEEILSLLNLY
                     HNLLREWSEI"
     gene            complement(7227..9509)
                     /gene="fiu"
                     /locus_tag="ACHOUK_001634"
     CDS             complement(7227..9509)
                     /gene="fiu"
                     /locus_tag="ACHOUK_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFP9897106.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTATLNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTASIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            complement(9774..10034)
                     /gene="mcbA"
                     /locus_tag="ACHOUK_001635"
     CDS             complement(9774..10034)
                     /gene="mcbA"
                     /locus_tag="ACHOUK_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFP9897107.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            10310..11236
                     /gene="rlmF"
                     /locus_tag="ACHOUK_001636"
     CDS             10310..11236
                     /gene="rlmF"
                     /locus_tag="ACHOUK_001636"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFP9897108.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELKQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVTFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            complement(11233..13458)
                     /gene="ybiO"
                     /locus_tag="ACHOUK_001637"
     CDS             complement(11233..13458)
                     /gene="ybiO"
                     /locus_tag="ACHOUK_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFP9897109.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEF
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQILSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLGSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPLPPGEPT
                     L"
     gene            complement(13575..14297)
                     /gene="glnQ"
                     /locus_tag="ACHOUK_001638"
     CDS             complement(13575..14297)
                     /gene="glnQ"
                     /locus_tag="ACHOUK_001638"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFP9897110.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            complement(14294..14953)
                     /gene="glnP"
                     /locus_tag="ACHOUK_001639"
     CDS             complement(14294..14953)
                     /gene="glnP"
                     /locus_tag="ACHOUK_001639"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFP9897111.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            complement(15091..15837)
                     /gene="glnH"
                     /locus_tag="ACHOUK_001640"
     CDS             complement(15091..15837)
                     /gene="glnH"
                     /locus_tag="ACHOUK_001640"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFP9897112.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            complement(16241..16744)
                     /gene="dps"
                     /locus_tag="ACHOUK_001641"
                     /gene_synonym="pexB"
     CDS             complement(16241..16744)
                     /gene="dps"
                     /locus_tag="ACHOUK_001641"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFP9897113.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            complement(17043..17930)
                     /gene="rhtA"
                     /locus_tag="ACHOUK_001642"
     CDS             complement(17043..17930)
                     /gene="rhtA"
                     /locus_tag="ACHOUK_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFP9897114.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            18165..18230
                     /locus_tag="ACHOUK_001643"
     CDS             18165..18230
                     /locus_tag="ACHOUK_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9897115.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            18283..18798
                     /gene="ompX"
                     /locus_tag="ACHOUK_001644"
     CDS             18283..18798
                     /gene="ompX"
                     /locus_tag="ACHOUK_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFP9897116.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(18847..20430)
                     /gene="opgE"
                     /locus_tag="ACHOUK_001645"
     CDS             complement(18847..20430)
                     /gene="opgE"
                     /locus_tag="ACHOUK_001645"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFP9897117.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTKGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(20702..20830)
                     /gene="mntS"
                     /locus_tag="ACHOUK_001646"
     CDS             complement(20702..20830)
                     /gene="mntS"
                     /locus_tag="ACHOUK_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFP9897118.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            21016..21483
                     /gene="mntR"
                     /locus_tag="ACHOUK_001647"
     CDS             21016..21483
                     /gene="mntR"
                     /locus_tag="ACHOUK_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFP9897119.1"
                     /translation="MSRRAGTPTAKKVTQLVNEEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            21480..22598
                     /gene="ybiR"
                     /locus_tag="ACHOUK_001648"
     CDS             21480..22598
                     /gene="ybiR"
                     /locus_tag="ACHOUK_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFP9897120.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVTL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(22656..23576)
                     /gene="ldtB"
                     /locus_tag="ACHOUK_001649"
     CDS             complement(22656..23576)
                     /gene="ldtB"
                     /locus_tag="ACHOUK_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFP9897121.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            23795..25387
                     /gene="ybiT"
                     /locus_tag="ACHOUK_001650"
     CDS             23795..25387
                     /gene="ybiT"
                     /locus_tag="ACHOUK_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFP9897122.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            complement(25555..26820)
                     /gene="ybiU"
                     /locus_tag="ACHOUK_001651"
     CDS             complement(25555..26820)
                     /gene="ybiU"
                     /locus_tag="ACHOUK_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFP9897123.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPSQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(26972..27787)
                     /gene="ybiV"
                     /locus_tag="ACHOUK_001652"
     CDS             complement(26972..27787)
                     /gene="ybiV"
                     /locus_tag="ACHOUK_001652"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFP9897124.1"
                     /translation="MSVKVIVTDMDGTFLNDVKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            complement(27933..30364)
                     /gene="ybiW"
                     /locus_tag="ACHOUK_001653"
                     /pseudo
     CDS             complement(27933..30364)
                     /gene="ybiW"
                     /locus_tag="ACHOUK_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
     gene            complement(30370..31269)
                     /gene="ybiY"
                     /locus_tag="ACHOUK_001654"
     CDS             complement(30370..31269)
                     /gene="ybiY"
                     /locus_tag="ACHOUK_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFP9897125.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDPRLCLEGCELCAKTAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQSLTVCGE
                     VKSVEEIMVTVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            31400..32062
                     /gene="fsa"
                     /locus_tag="ACHOUK_001655"
     CDS             31400..32062
                     /gene="fsa"
                     /locus_tag="ACHOUK_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFP9897126.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLEVVLP
                     ELHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTTEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPRA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWQGAFGRT
                     SI"
     gene            complement(32241..32990)
                     /gene="moeB"
                     /locus_tag="ACHOUK_001656"
     CDS             complement(32241..32990)
                     /gene="moeB"
                     /locus_tag="ACHOUK_001656"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFP9897127.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDSVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHNLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFSENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(32990..34225)
                     /gene="moeA"
                     /locus_tag="ACHOUK_001657"
     CDS             complement(32990..34225)
                     /gene="moeA"
                     /locus_tag="ACHOUK_001657"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFP9897128.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADITSGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLHRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VEVGEWVEVEPFNALFGGL"
     gene            34429..35394
                     /gene="iaaA"
                     /locus_tag="ACHOUK_001658"
                     /pseudo
     CDS             34429..35394
                     /gene="iaaA"
                     /locus_tag="ACHOUK_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
     gene            35381..37252
                     /gene="gsiA"
                     /locus_tag="ACHOUK_001659"
     CDS             35381..37252
                     /gene="gsiA"
                     /locus_tag="ACHOUK_001659"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFP9897129.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKEAVARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            37272..38810
                     /gene="gsiB"
                     /locus_tag="ACHOUK_001660"
     CDS             37272..38810
                     /gene="gsiB"
                     /locus_tag="ACHOUK_001660"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFP9897130.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            38828..39748
                     /gene="gsiC"
                     /locus_tag="ACHOUK_001661"
     CDS             38828..39748
                     /gene="gsiC"
                     /locus_tag="ACHOUK_001661"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFP9897131.1"
                     /translation="MLNYVIKRLLGLIPTLFIVLVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIARRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            39751..40662
                     /gene="gsiD"
                     /locus_tag="ACHOUK_001662"
     CDS             39751..40662
                     /gene="gsiD"
                     /locus_tag="ACHOUK_001662"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFP9897132.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
BASE COUNT         9718 a        10140 c        11007 g         9937 t
ORIGIN      
        1 taatgcgctt atcgcaccag gcgttaatct caatgcttga gccagaggtt aagaaaaaga
       61 ctatctggaa taattactct atttatccat ctttgcagga tacccatgaa gttgtcagag
      121 atgatccaga aacgatttgt atgcgtgcat ttcctttatt tgcaaaagga tgggaatatg
      181 cgcagaaaaa taagaaacat caattgatcc tcaatgctct tggattcaaa ggttatattc
      241 gtgatatatt tatgtcagca ataatgcgaa aaacagattt tgtgctcgaa tgcaataatc
      301 aaccgacaga acttaattca tcattttcct ctttgatgaa cgattcagat caatggcaac
      361 agcatactct gaaagataaa cattacgcta atctattaac gatgctggat ctcaatgatg
      421 ccagtgaaag tgataagtct aaaatatttt tctgcctgtc tgccgtattt gcaaatattt
      481 ctcacagtaa tgtctttaac ggtattcctg atgcgtcaaa aactttaaaa cgttatgcat
      541 ttgctttatt ggcgaaagct cattctttag atgaaagtat gataagcaat caaactttta
      601 atacatacaa aacggtattg cttgatttca ataatctgtc taatgaagag gccaaccagt
      661 tgcgcataag ttcgttatat cgtgatatgg tccgctatgc acaatatcgt ttttctaaag
      721 ttttatccga gtggacgcca gacgcatggc tgtaaagatc gcagcagaat aatggcattg
      781 atggccctgg ctcaggccag ggtcggcaat accggttcct gcgaagcgat gacggcggca
      841 attgtaacca cgaaatgcgg tatcacttgc tgttttctgc cctgaagact ggcgctcact
      901 gtgccgtcga attttcaatc aaatatattg cgagagggcg tgtttgcgag ccgctttcca
      961 gaaacagaaa aaccattacc cctgaaaacc gaaaaatgcc acaatattgg ctgtttatac
     1021 agtatttcag gttttctcat ggcattaacc gccgcgctta aagcgcaaat tgccgcctgg
     1081 tataaggcgc ttcaggaaca gatccccgac tttattcccc gtgcgccgca gcggcagatg
     1141 attgcggacg tcgccaaaac gctggccgga gaagaagggc ggcatctggc gattgaagcc
     1201 cccactggcg ttgggaaaac gctctcctat ttgattcccg gcatcgccat tgcccgcgaa
     1261 gagcaaaaaa cgctggtggt gagtaccgcc aacgtggcat tgcaggatca gatctacagc
     1321 aaagatttgc cactgctgaa aaagatcatt cccgatctta aattcactgc cgcttttggg
     1381 cgtgggcgct acgtttgccc gcgtaatctg acggcgcttg ccagcactga gcccacgcaa
     1441 caggatctgc tggcgtttct tgacgacgaa ctgacgccaa ataatcagga agagcaaaaa
     1501 cgttgtgcga agctgaaggg cgatctcgac acttataaat gggatggtct gcgcgatcat
     1561 acggatatcg ctatagatga cgatctctgg cgtcgtttaa gtaccgacaa agccagctgc
     1621 ctcaaccgca actgttacta ctatcgcgaa tgcccgtttt ttgtcgctcg tcgggaaatt
     1681 caggaagcgg aagtggtggt ggcaaaccat gcgctggtga tggcggcgat ggaaagcgaa
     1741 gccgtattgc ctgacccgaa aaatttactg ctggtgctgg acgaaggcca tcacctgcca
     1801 gatgtggcgc gggatgcgct ggagatgagt gccgaaatca ccgcgccatg gtatcggcta
     1861 cagctggact tgttcacgaa actggtcgct acctgcatgg agcagtttcg cccgaagacc
     1921 atcccgccgt tggcgatccc tgaacgtttg aatgcgcatt gtgaagagct gtatgagctt
     1981 atcgcctcat taaacaacat tctcaatctc tacatgcctg ccgggcagga agcagaacac
     2041 cgttttgcga tgggcgaact gcctgatgaa gtgctggaga tctgccagcg gctggcaaaa
     2101 ctcaccgaga tgctgcgtgg tctggcggag ttatttctta acgatttaag tgagaaaacc
     2161 ggcagccatg acattgtacg tctgcatcgg ttgattttgc agatgaaccg cgcgttgggg
     2221 atgttcgagg cgcaaagcaa actctggcgg ctggcttcgc tggcgcaatc ttccggtgca
     2281 ccggtgacca aatgggcgac gcgggaagag cgcgaagggc agctacacct ctggtttcac
     2341 tgcgtgggaa tacgtgtcag cgatcagctg gaaaggctgc tgtggcgcag tattccgcac
     2401 attattgtca cctccgcaac cttgcgttcg ctgaacagtt tttcgcgttt gcaggagatg
     2461 agcgggctga aagagaaagc gggcgaccgt tttgtggcgc tggattcccc ctttaaccac
     2521 tgcgaacagg gcaaaattgt tattccccgg atgcgcgttg agccttccat cgacaacgaa
     2581 gaacagcata ttgctgaaat ggcggccttt ttccgtgagc aggtggagag caaaaaacat
     2641 ctcggtatgt tggtgctgtt tgccagcggg cgtgcgatgc agcgctttct cgactatgtg
     2701 acggatttac gtctgatgtt gctggtgcag ggcgatcagc cgcgttaccg tttagttgaa
     2761 ctgcaccgca aacgcgtcgc caacggtgag cgtagtgtgc tggtgggctt acagtcattt
     2821 gccgaagggc ttgatttgaa aggtgatatg ctcagccagg tgcatatcca caaaatcgct
     2881 tttccgccta tcgacagccc ggtggtgatc actgaagggg aatggctgaa aagcctcaac
     2941 cgctatccat ttgaggtgca aagcctgccg agcgcctcgt ttaacctgat tcagcaggtt
     3001 gggcgactga ttcgaagtca cggttgctgg ggcgaagtgg tgatttacga taaacgcttg
     3061 ctgaccaaaa attatggcaa gcgactactg gatgcattac cggtatttcc gatagagcaa
     3121 ccggaagtcc ctgaaggtat agttaaaaag aaagaaaaaa cgaaatcccc acgccgtcgg
     3181 cggcgttaat gatgtgagtc aggtaaggag tcgtaaatgg actatcgcaa aatcattaaa
     3241 gagatcgggc gcgggaaaaa ccacgcgcgc gatttagacc gggatactgc gcgcggtctg
     3301 tatgcccata tgctcaatgg tgaagtccct gaccttgagt tgggcggtgt attgattgcg
     3361 ctgcgtatca aaggggaagg ggaagcagag atgctcggct tttacgaagc catgcaaaat
     3421 cacaccatca agctgacgcc gcctgcaggc aagccgatgc cgattgtcat ccccagttac
     3481 aacggcgcgc gtaaacaggc caacctgacg ccgttgttgg cgattctcct gcacaaactc
     3541 ggttttcctg tggtggttca cggggttagc gaagatccaa ctcgcgtgct gaccgaaacc
     3601 attttcgaac tgatgggtat tacgccaacg ttgcacggcg ggcaggcgca ggcgaagctc
     3661 gacgaacatc aaccggtgtt tatgcctgtc ggcgcgtttt gcccgccgct ggaaaaacaa
     3721 ctggcgatgc gctggcggat gggcgtgcgt aacagtgcac ataccctggc gaaactggcg
     3781 acgccatttg ccgaaggtga ggcactgcgt ctttccagcg tttcgcatcc ggaatacatc
     3841 gggcgagtcg cgaagttctt tagcgatatc ggcgggcggg cgttactaat gcatggtact
     3901 gaaggcgaag tgtatgctaa tccgcagcgc tgcccacaga tcaatctcat tgatcgtgaa
     3961 gagatgcggg tgctgtatga aaaacaggac cctgctggaa gcgagttact gccacaagca
     4021 aaagatccgg aaaccacggc gcagtggatt cagcgttgcc ttgctggccg cgaaccgatt
     4081 cccgaatcgc tgaaaatcca gatggcttgc tgcctggtgg ctacgggtga agcggcaact
     4141 atcagcgatg gcctggcgcg cgttaatcag gcattttaat tcttttctca gccggatgag
     4201 ccatgtcatc cggcttttac cccaccgcaa tatgaaattc ctgcatcttt attgaccttc
     4261 ccgcgcccgg cgtgcagcat aaaaaaacaa caaacacata acataaacag gagttaacca
     4321 tggaaagtgg tcatcgcttt gatgctcaga cgctgcacag ctttattcag gctgtatttc
     4381 gtcagatggg tagcgaggaa caagaagcga aattagttgc cgatcattta atcgcggcaa
     4441 acctggcagg gcatgattca catggtattg gcatgatccc aagctatgta cgctcctgga
     4501 gtcaggggca cctgcaaatt aaccatcatg ccaaaatcgt taaagaggcg ggggcggcgg
     4561 tcacgctcga tggcgatcgc gcatttggtc aggtcgcggc acatgaagcg atggcgctgg
     4621 ggattgagaa agcgcatcag cacggtattg ctgccgtggc gctacataac tcgcatcata
     4681 tcggccgtat cggttactgg gcggagcagt gtgcagcggc ggggtttgtc tctatccact
     4741 ttgttagcgt ggtcggtatt ccaatggtcg caccgttcca cggtcgcgac agccgctttg
     4801 gcacgaatcc gttctgtgtg gttttcccac gtaaagatga ttttccgctg ttgcttgatt
     4861 acgccaccag cgccattgca tttggcaaaa cccgcgtcgc ctggcataaa ggcgtccccg
     4921 tgccgccagg ttgcctgatt gacgttaacg gcgtgccgac gaccaatccg gcggtaatgc
     4981 aggagtcgcc gttgggttcg ctgttgacct ttgccgaaca taaaggctac gccctggcgg
     5041 caatgtgtga aattcttggc ggggcgcttt ctggcggtaa aacgacgcat caggaaacgt
     5101 tacaaaccag tcccgatgcc attcttaact gcatgaccac tatcatcatc aacccggaac
     5161 tgttcggcgc gccggattgt agcgcgcaga ccgaagcctt tgccgagtgg gtgaaagcct
     5221 cgccgcatga tgacgataag ccgattttgc tcccgggcga gtgggaagtg aacacgcgtc
     5281 gcgaacggca ggagcagggg attccattgg atgcgggaag ctggcaggcc atttgtgatg
     5341 cggcgcggca gattggtatg ccggaagaga cgttgcaggc tttctgtcag cagttagcca
     5401 gctaaaaaaa agcccgtcca gtggcggacg ggcaaacaag ggtaacatag gatcaatgag
     5461 ggttagagca tatgcgtctg tcggcaaaca gacagggaaa tacttgtgca ggacgtagcg
     5521 taaacgcctg atccgtccaa cggttcggat tttgtaggcc tgataagacg cggtagcgtc
     5581 gcatcaggca tctgttgccg gatgcggcgt aaacgcctta tccgtcctac gaattactta
     5641 taaattaccg cagtaccgct taatttattg ttgttggtgg cggaagtgat gctatatcca
     5701 ctggcaccgg ctgccgccgc tttctcagcc agtttcgctt ccagggcatc gagggtggat
     5761 gcgccatcgg cagaaaccac gccgatttta ttcatgttct gtgcctggga tgccgttacc
     5821 ggttccgcgg caaacacgcc aaatgacaga gctgaaagag ccatagcagc aacaacagta
     5881 ttgatagttt tcataattaa tctctcgcag gtgatttttg tataagtgac gttgtttcgt
     5941 cgatgtgatg agtatcacgt ttttttacga gagataaaat cgaagagaat tgaccgcctt
     6001 gttcaaataa attgattgat atctaacata ctgataaacc atttatttgt tgtacttatg
     6061 gacttgtttt atgcagcgtg gcagtcacga cagcgttaaa ggcacggaat gccacgcaaa
     6121 atacaaatta tactcgctat acatctcgcc agttgtagtt aacgtaactg gctttctttc
     6181 gaacctctgc gattatatcc tgtataagct ggtttttgta aatctttctc ctgctgacaa
     6241 tgaatgcata agcgcacgcc aggaatggct tcccgacggg cttgcgggat gggggcaccg
     6301 cactcttcac attcatacag gctttcgcca cgcggaattt caccccgagc gcgggcaatc
     6361 gcatcttcaa ttgtactgtt gatctgttcg ttgacggcgt catcgttagc ccaaccggat
     6421 gccatatcgt cctccccata tcaatgcttg tatagttaag tgtagctaat atagggacga
     6481 acctgggcag ttcaagcgtc agatctccga ccattcccgc agcagattat gataaagatt
     6541 gagcagcgac aggatctctt cattttcacc atagcggctt ttcagcgact gaatattctg
     6601 aatatttttg tccagttcaa acagcatggc gcgcttttta tcatcgcgga tcatcgactg
     6661 gatccatata aatgatgcca ctcgtacgcc gcgggttacg ggtgtcacgc aatgcaggct
     6721 gctggaggga tacaacacca gatcacctgc cgggagtttt acccgatgtt gtccgaaggt
     6781 gtcattaacg accagttcgc cgccgtcgta gctttgtgga tcgcttaaaa acagcgtggc
     6841 agaaaggtca gtgcgcatcc agccgttttg tggatggctg cgtactgcgc catccacatg
     6901 aaaaccatag gtttcattgt tctgatagcg attaaacagc ggcgtggaaa gggtacgcgg
     6961 caaggccgcg gcaaagaaca aagcatgttg gttaaccgcg ttcagcacct cattttgcag
     7021 ggcggcgtat aacgtgctgc gggtgtctac ctgttgattg ttcttaactt gcgcaccttg
     7081 tgcgccggtg gtgacgcgtc catccaccca ttcggcttgt tccagttgtt cgcgaaaacg
     7141 ggcgacgtcc tgtggcgata acacgccggg aatgtggtac atcatacaag ttctccaaaa
     7201 agtggggcct gcgccccaca tctgaatcag aaatgcatat tggctgtgag caagaaggtt
     7261 cttggctcgc ccgggtgata acggtagccg ctcttattga ttgaggcgac gtaatcggta
     7321 tcaaacaggt tgtagacgtt tagctggaag tcgagattgc gattaaccct ataccccagt
     7381 ttggcatcgg cgacccagta accttcggta aacgctggcg ttcccaccgc gccgtctgaa
     7441 cctttatgca tactgccgat atagcgtgcg cccgcgccaa cagagatatc atcggttgcc
     7501 tgatattggc tccataaggt gaaggcgtgc tccggggtat acggcagcga tgaggaacca
     7561 tcctgggcaa catctttgcc gtttttgatg gttgcttttt gctgggtata gccgccaatc
     7621 acctgccacg cgggagtgat attccctgcc acggatatct catagccttc gacgcgtttc
     7681 ttaccgtatt gcgagtaagt cccgtcatca ttttgctcaa cttcattttc gatatcagta
     7741 cggaacagcg cggcggtgag caacaggcgt ttatccagaa cctgccattt ggtgccaatc
     7801 tcgctggtgt tggctttttg cggtttaaaa tcggtgcggt tggcactgtt accgctgcca
     7861 gactgcgcaa gggcgaagtt gttgccgccc ggaggctgct gggaaacggc atagttaata
     7921 tagacattgc cgttttccgt caggtgatac agcgccccgg ctttccagtt caccagattg
     7981 cccgacttgg cggtgtcgac ggtggtgacc ggagaacctt ttgccacacc agctgggcag
     8041 gtgatggcac cgcgtccgct gccgccgcag gcggtggcac tgtcatattc agtatgataa
     8101 ttatccagac ggatcccgcc gttcagctca aaatcacggg tgatttgcag cgtatcgaat
     8161 gcgtaaattg cgaaggtatc cgtctgacca ttggcgtttg cgccgttgcg cgtcaggccg
     8221 ccgggatgaa tgctgctgtc aggatgataa atatttaccg caggtaacgt caccggatta
     8281 acgccgtagt tagtctgcgt ttcacgggta aattccacgc cggtactgac atcatgaccg
     8341 atagaagcgg tatagaacgt cgaggtcagg ttggtctggt tggtgagaat tttattactc
     8401 acatctttgg tattcgccgt gcgtgaccac gtccagctat tcacatcgct ggtgggctgg
     8461 gtaatattcg acgccccacc cataatcgcc gtcatcaggt aatcctgctt cacgcgcgac
     8521 caacgggtag tgttgcgaat ggtggtgtta tcgttgatat cgtgctcaaa acgcatggtg
     8581 gcggtgtcgg tggtcgaatc gtcgtaatcg gaatccgtgc cgtaaaagtt atgcgtatca
     8641 acttttccgg aatgattcag ggtcgccgtt cctgcagatg gggcagaata gcccggcaaa
     8701 ccgatggttg gaatgccgcc gtctggcgtg ttgtgctggg tgacatgcag ataattaaga
     8761 tacaaacgat tcgctgtacc aaggccaaaa gcgatagaag gggcgacgcc gtaacgctca
     8821 tttttgactt tgtcgcgtcc ggcatcgtgc gttttttcgc ccatcacatt caggcgcacc
     8881 gcagtggtat caccaatgac ctgattgacg tccagcgtgc cgcggcggaa ccaggcgctg
     8941 ccaatactgg cggaggcgtc aatgccggaa tcattgcgcg gctgcttgct gatcatattg
     9001 atcgagcccg tcggcgcgct gcgcccgtag tcggtgccgg aaggcccttt aatcacttcg
     9061 acctgctcag tattgaaggt gtcgcgcgag acgctgccga tatcgcgaat gccatcaata
     9121 taaatactgt tagaggtatc agcaccgcgc atataaatgg cgtcgccagt ggtggagtta
     9181 ccgttctcac ccgcaaaaaa cgcacccacg ccggggacgt ttttgagcgc atcggtaagg
     9241 ttggttgcgc cctgatcttt aatcacttgt tcagaaatca ccgtcatcgt gcgggtagta
     9301 tccgctaccg gacgcgagaa tttcggatcg gcagattgtt gtggcgcata aagcgaaggc
     9361 gttgatgctt cgacaaccag cgtgtcatcc gcgttagttt gcccttcggc ggcgagtgcc
     9421 tgagccacag gcgtgatgcc aatacaaaga ccggcaaaga acgtgagcga atgaaattgt
     9481 ctggcaggga aattgcgatt gttttccatt ttgcaggtga ctttttctta tatgttgaaa
     9541 ttttcactgc cacgagaaag cctctctgcg gatgcgatgc cactgttaat ctcgtgatag
     9601 tgttcgtgca agtgataata attttgataa gcattatcac ttggcgcgat tttctatcac
     9661 ccattgcgcg aaaaagaaat acatttattt acattgcgtt catatttgat gttttttgat
     9721 tacaaaacag gcgaaaactg catccagagg cgaatgcagt tacgttggtg gggttatttg
     9781 tagatggttg cagtaccgaa catctggtca tttccgcctg cggaattaat gacataacct
     9841 ttcgcgcctt gctcgcgcgc tttttctgcc agtttatcct ccagatcgct taggtttgat
     9901 gcgccagttg ccgaaacggt accggcgggc cgtagctgac cgctgtccag gttactcatc
     9961 ggttgcgcgg cataagccgt gagagagatt ccgctcagga cggtggcaat cagtagtgtg
    10021 aggcactttt tcataatgac atcctcataa atacaacggg tagtgcctga taagtgtagg
    10081 tcgcctgcgc gatgcaaatg acgcaaaaaa ttgacagaga tgtgcttatt ttttgaacga
    10141 tatattttta caaaataaga caaatcaatg acatgcataa tgctgatttt tatgatgaat
    10201 tgccataaac catagccatg ttaaggtttt ggggaatgat aaacgcagcc agatatttac
    10261 cctgtcgcgt tatgcgggta tcttacgccg ctgcttaaag gagaatgcta tgtccgccca
    10321 gaaaccgggg ttgcatccgc gcaaccgtca tcacagccgc tacgatctcg ccacgctttg
    10381 tcaggtcaat cctgaactca agcaattcct cacgcttaca cccgccgggg agcaaagcgt
    10441 agactttgcc aatccgctgg cggtgaaggc gctcaataag gcgttgctgg cccattttta
    10501 cgccgtagcg aactgggata tcccggacgg ttttctctgc ccgccagtac cgggccgggc
    10561 ggattatatt catcaccttg ccgatttact ggctgaagca agcggaacaa ttccagctaa
    10621 tgccagcatt cttgatatcg gcgttggtgc gaactgtatt tatccgctga ttggcgtaca
    10681 tgaatatggc tggcgtttta ccggtagtga aaccagcagc caggcgttaa gcagcgcgca
    10741 ggcgatcatc agtgctaatc cggggcttaa tcgcgccatt cgtctgcgtc ggcaaaaaga
    10801 gagtggggcg atttttaacg gcatcatcca taaaaacgag caatacgacg cgaccttgtg
    10861 taacccgcca ttccacgatt ccgccgctgc ggcgcgggca ggtagtgagc gtaaacgccg
    10921 taacctgggg ctgaacaaag acgatgcact gaactttggc ggccagcaac aggagttgtg
    10981 gtgtgaaggc ggtgaggtca cctttatcaa aaagatgatt gaagagagca aaggcttcgc
    11041 gaagcaggtg atgtggttta cttcactggt atctcgtggt gaaaacttac cgccgttgta
    11101 tcgtgccctg acggacgtgg gcgcggtgaa ggtggttaaa aaagagatgg cccaggggca
    11161 aaagcagagt cgctttattg cctggacctt tatgaacgac gagcagcgcc gccgttttgt
    11221 caatcgccag cgttaaagcg ttggctcccc cggcggtaac ggttcagccg gggtcgcgcc
    11281 cggagcaggc agcacctgat aagtctgcac cggcgcgcga acgcccgcca ggtcgaaatg
    11341 ttttttcacc tggctgtcga gggcaaagcg taccgtccac tgtttgagtg gcagcgtggt
    11401 gaacgaaaca cgcagtgtaa acgcggtatt gcttaagcca acaatcccgg caaagttcgg
    11461 ttcaccaata atcagcccac gaatttcttc gttttccatt aattccgcta ccgcatcttt
    11521 cagtgcctga ttggctttat cagcatcttc atggcgatca acatcgtagt ttgccactac
    11581 cgaaccaatg ccgcggacaa agttggcaaa ggtggtaatc gaagaccacg gaatgatgtg
    11641 gtacgccccg gtatcctggc gtacgcccac ggagcgaatc gacatccgtt ccacagtgcc
    11701 ggtcaacggc ccaatagtca ccaaatctcc agtgttcatg ccgttttcaa actgaataaa
    11761 taccccggtg ataatatctt tcaccagcgt ttgcgaacca aacgagatag ccagacctaa
    11821 tgccccggca cctgccagca atggcgcgat attgacgccg atttccgaca acacaatcat
    11881 gatggtgatg gtactgataa tcaccgccag cgcgttacga aacagcgtaa gcagggtacg
    11941 cgtgcgggcg ctgggtagcg ggcggccatg aatatccgaa gccagccggt tttcgatcaa
    12001 actggcgagc accgtccagc caaccgccga gaagaaaaga atgagtgcga tacggatcag
    12061 gatatctacg gttttctgcc ccgcgccgtt ttgcagccag ttccagaaat cgaacaatcc
    12121 ccatgcgctc aacagcagca ttaccgccac gcagacggtc agaatacgcg ccgttttcag
    12181 cgccgccgaa agccagccat tcagccgttt ttgcaactcc ggatagttac gctgagtatg
    12241 tggcgagaga gtgatggttt ttgccagcca gcgggaaaac ataccggaaa caaatgctgc
    12301 gataccaata atcgccaggc tgcgcactgt tgcacccatc ataaatttca ggctgttgcc
    12361 cggatcgaac aacgaaaaga aaaacagcac gataaaatag gcgcttgcca gccagtgcca
    12421 caccagcgca aaggcgcgga taaacaggct gaaaaaggcc agcgaatgct ccgcaaagtt
    12481 gagcaaatgc tgggtaatct cttttttatt acgaaagatc aggtacaacg cccagacggt
    12541 catgcacagc ataatgatga cgttcgccag cgcacctatc tgtacattca cctgattaga
    12601 gataatcggc acggccacaa tcaggccata acctatcaaa ctgcctaacc agctcaggcg
    12661 acgactccag taacgggcgc tctcgtcctg aatcgtgaac gggcgcagtt ccgccacgtt
    12721 tgggcaaaaa atcaggcgta gtacggcttt gaaaaattca atcagggcaa aggcgttgag
    12781 aaacaaactt tgttggaaag cgatggtgcg actgcctgca ttcaggttgt cgcttaatat
    12841 ctggccgaca aataatgtca gtgccagtaa cagcaggtcg ataataaacg ccccgataat
    12901 catcgccgga agctgcagcc agttactgcg ctcacgattt ttttgccgcg cccactgacc
    12961 cattttgcga tacagcggca gtgcgcacag gcgaatcagc cagtaaaaac caaacactaa
    13021 aaccgctaac attgaaaaat gggtcagcgc attgctgaag gtttgtggat taaacggctt
    13081 atgcggggag ccggtgatat tgcgataaag ctgcccgaag cgggcggaaa gggcttcacc
    13141 ataatggcgg ctgaattcgg tgaccttttg cagcacggtt tgctcttcaa ccagcgtcgg
    13201 cggcacgatc ttcgggaccg gttcagcagg tggcgtcgcg gcgacggtgc gcaactggtc
    13261 gatcaactct ttacgcgagg tgtcattatc cagcacatcc gccagtgcgc cataagccgc
    13321 ttttttttgt tcgatatccg gttccggggc cggttcagtc gttgagtccg ttgtcgttgt
    13381 ggttgtaacg ccgggtatgg ataccgcgtg ggcaggtgcg cccagcaggc agaagaggat
    13441 gaacaggatc caccgcatga ctcctccagt gagaaaacag agcaaaaaga taagtataga
    13501 tgctggggga ggggtgattt taaatgagag gaatctggtg tacctccctt tcgggtgaaa
    13561 gggaggcaga gaagttaaga gacgtgctgt aaaaattcct gcaagcgctg gctcggcgga
    13621 tttttaatca acacttgtgg atcgccatct tccgcaatcc ggcctttgtc gataaagatc
    13681 aggcgcgaag ctactttctc ggcaaaaccg atttcgtggg tcacgatcac catcgtcatc
    13741 ccttcttcag ccagatcttg cataaccttc agcacttcat ggcgcagttc cgggtcaaga
    13801 gcggaagtcg gttcatcaaa cagcatcatt ttcggcttca ccgccagcgc gcgggcaatc
    13861 gccacacgct gctgttgacc accagaaagt tcggaagggt agtgatgtgc acgttctgcc
    13921 agaccgactt tcgccagcag ctcacgtgcc agtttttccg cctcttcttt gttcgcgcca
    13981 cgcacgcgta gcgggccaaa catgacgttt tccagcgccg tcagatgcgg gaagaggtaa
    14041 aactgctgga acaccatacc tgcttcctgg cgaatcaggc gctcgtcaac tttcggatcg
    14101 ttaaccttca ggccatcgac aatcagatcg ccggaggtga tttcttccag tttgttgatg
    14161 cagcgcagca gggtcgattt accggaaccg gacggcccga taatcaccac gacttcgccc
    14221 tgggcaatgt tcaaatcgat attgtgcagc acctgggttg ggccaaagtg cttggagacg
    14281 tttttaaatt caatcacagg attttcatcc ttctttccag acgacgcaga ataaagctca
    14341 gcaccagggt aataatcaga tagaacaccg ccacggcgct ccagatctca agggcgcgga
    14401 agttaccggc aataatttct tgcccctgac gggtcagttc cgccacgccg atcacaataa
    14461 acagcgaggt gtctttaatg ctgatgatcc actggttacc cagcggcggc agcatacgac
    14521 gcagtgccag cggtaaaatg acgtagcgaa tggtttccca acgtgaaaga ccgagcgcca
    14581 gtcctgcttc acgaaaacct ttgtggatag acagcaccgc accacgcgtg atttccgcaa
    14641 tgtacgcgcc ggagttgatc atgatggtga ccaccgccgc agtaaatggg tcgatgcgta
    14701 agtcattaaa cgccatcggc agggcaaaat agataaacat cacctggacg acgataggtg
    14761 tgccgcggat cacttcaata aaaaccagcg cgacgtggtt ggctatccaa ccaccgaagg
    14821 tgcgtgcaaa acctgccagc aatccgatca ccaggccgcc agccagaccg aggaccgata
    14881 tccacagggt cattttggca ccttcaatca gaagcggaat ggcaggccag atagcactcc
    14941 agtcaaactg catatgttgt tcctgttacc gtggtgtaaa taatcaaatt cagggtaatt
    15001 aatggccccg gagcgggttt gcgccctcac cctaatcctc tccccagagg ggagagggaa
    15061 ctgccagtgc gctttacagg tgtagcgtta ttatttcggt tcagtaccga accatttttt
    15121 gtagatttcg ttgtaagttc cgttctcgcg cagggttttc aacgcgccgt tgactttgtc
    15181 acgcagctcg tcgctacctt tcgggaacgc aataccgtat tgctgcgctt ccagagagtc
    15241 gcctaccgct ttgaactgac cgttaccggc ggttttgatg aagtacagaa tgtttggcgt
    15301 atcgtgcaga acggcgtctg cgcggttggt gcccagttcc atataggcgt tatcgatgtt
    15361 cgggaactga cgcagatctt tagttttgat gtttgctttc gcgtaatcaa cggagccagt
    15421 gccgctcttc accgcaacca ctttcccgtc gagatctttc acgcttttca catcgttatt
    15481 gttagctttc accatcacta acaggccgct tttgtagtag ccgtcagaga aatcgatcgc
    15541 ttttttacgc tcgtcggtga tggtaatgcc cgccagcgcc agatcgacgt ttttggtttg
    15601 cagtgccgga atgatcccac tgaaatccat cggcttcagt tcgtaatcca gcttcagctc
    15661 tttagcgatg gcagcccaca gatcaacgtc aaagcccaca tatttatcgc cctgtttaaa
    15721 ttcaaacgga acgaaggcgg tatccgtcgc gacaactaat tttttatccg cggcatgaga
    15781 agaaaccgca aaagccaggg tcagtgcagc cagtgaaact tttaatacag acttcatagc
    15841 atttcctttt ttctatccac gggacgatcc cctgcgtgac attcacatat atgaaaaaat
    15901 cgtgccagtt ttgcaactca ttgatttttc gagatgcggc aaaaacgtga tgcacgattt
    15961 atagggcaac accctgaaga tgcaccattc tggggcacca aactggtgcg ctaaaattgt
    16021 gcactcaaca cagtgattat ttagcgtaaa acctgatgaa aaaacaatct ttatgtaacg
    16081 attgtgtgat gatgtggata caaaaaattt acttaatcag ctggggatag cagatggatg
    16141 cactaaataa gtgcgttgag gcgggctgca aaacaaaacg gcctcctgtc aggaagccgc
    16201 ttttatcggg tactaaaatt ctgcaccatc agcgatggat ttattcgatg ttagactcga
    16261 taaaccacag gaatttatcc aggtcgcgag acgcggcggt caggatatct gcggtgtcgt
    16321 catctttcgc ttcgccaatc gctttgcgta cgtcattagc gacgattgcg taacggtcag
    16381 ccagttcttt caggtgatcc tgaacgttgt ggatgtccag cgggtaactt ttcagcgggg
    16441 ttttgctgtt gataacttga gtggtcccca gagctacacc gcccagctgc actgcacgtt
    16501 ctgccatggt atccagatga tcgatcagtg cagtgcggaa gccatccagc atttcatgta
    16561 cggcaatgaa gttagcgccg cgcatgttcc agtgcgcttg tttggtaatc aaagaaagat
    16621 caataaactg gataacctgg cgattcagca actctactgt tgcttttttc tcgctgtcag
    16681 agacatcgtt gcgggtataa agcagattgg tcgcttttga tttaactaat ttagcggtac
    16741 tcataatttc atatcctctt gatgttatgt cccagtaatt aacgagacta agtatagcac
    16801 cggctatgtg ttccgctatt ctggctgttc ctatcacact aatagtggta acaagcgtga
    16861 aaaacaaaac taataaagat tcaatgagtt agatatattg ataagaacaa ttcttattta
    16921 tttgctcgcc aggaattttc ggaataaata tgcttttgca taattcgata tataaagacg
    16981 gtgtagagga aaagtagcga gaaattctgc atggttatgc ataaccatgc agaaatgtaa
    17041 atttaattaa tgtctaattc ttttattttg ctctctttgc gtactgtcag cgtagaaccc
    17101 attgaagcgg cgataattgc gccgagcgcc agcagctgta tgggtgtcag tgtttctccg
    17161 aggaaaatca tcccggaaac ggcagccagc gccggttcca tgctcatcag cgtaccaaat
    17221 gtccgtgttg gcaaacgggt gagggcaatc atttccagcg aataaggcag agcggtcgag
    17281 agaatagcga ccgccagacc caatggaata accgaccagt gccagagtgc ttcaccagcc
    17341 tgaagcgctc caattggcac gaaaattaac gctgcaatca acgaaccaat tgccaccgtc
    17401 gcagggccat gttccgctcc tgcgcgttgc ccacttaaaa tgtaaatagc ccaacaagcc
    17461 ccggccccca gtgccagcgc acagccggtt aaatcgacat gggaaacgtc ttgccccagc
    17521 ggtagcagga accacagacc aagaaccgcc agcacaaccc agacgaaatc taccgggcga
    17581 cgagaagaga acagcgccac cgccagtggt ccggtgaact ccagcgccac cgcaataccc
    17641 agcggtaccg tctgaataga gagataaaaa agataattca tcccacccag cgaaacgccg
    17701 taaaacaaca gcggtaaccg ttgctctttg gcaaagcgca gtcgccatgg cttaaagaac
    17761 gcgatgagga tcagcgttcc taatgccagg cgcagcgcag tgacacccgg tgcgcccacc
    17821 agaggaaaaa gtgacttagc taacgaagct ccaccttgaa tagacgccat ggcaacgagc
    17881 aatatgacta ttggtaacca gaccggcatt ttacgtaatg aaccaggcat cctttctccc
    17941 acaaatatct agacttaagt aaagcgtgga gtgtactgga tatacccaat gctggttgag
    18001 catttgttga aaaaattttc ccccgttttg actaaaatgc gccaggattg atggaatcat
    18061 tagtctggtg attaggaata atctggatga atgacaggga aaacatgcgt aatacttacg
    18121 cagttctctg aaaaagtgat ttaaatttag atggatagcg gtgtatggaa acgttctgtt
    18181 acatgaaatg gcccgttaga catcacaaat cgcgaagagt ttcccattaa tttttgatat
    18241 atttaaaact taggacttat ttgaatcaca tttgaggtgg ttatgaaaaa aattgcatgt
    18301 ctttcagcac tggccgcagt tctggctttc accgcaggta cttccgtagc tgcgacttct
    18361 accgtaactg gcggttacgc acagagcgac gctcagggcc aaatgaacaa aatgggcggt
    18421 ttcaacctga aataccgcta tgaagaagac aacagcccgc tgggtgtgat cggttcattc
    18481 acttacaccg agaaaagccg tactgcaagc tctggtgact acaacaaaaa ccagtactac
    18541 ggcatcactg ctggtccggc ttaccgcatt aacgactggg caagcatcta cggtgtagtg
    18601 ggtgtgggtt atggtaaatt ccagaccact gaatacccga cctacaaaca cgacaccagc
    18661 gactacggtt tctcctacgg tgctggtctg cagttcaacc cgatggaaaa cgttgctctg
    18721 gacttctctt acgagcagag ccgtattcgt agcgttgacg taggcacctg gattgccggt
    18781 gttggttacc gcttctaatc actttggtga tataaaaaat ccgcctctcg gggcggattt
    18841 ttgtttttaa ggcttcgggt cgaaaatatc ggttccgaga tggttgtaat ccaccttctg
    18901 taactggaag ttggtgatgt atatcggccc ggctttcttc tcagatataa acgggtattt
    18961 gatgttaatt tccttcgctt taattcccgt ccactgcgag aaaaagccta agaagtcatt
    19021 ggctgagcgg cgggctttaa tcacccgatg cgctttatcg tcgctggaaa tgaccataaa
    19081 aggcacctgg aaattttgct gatacttatc atcatgggca aggtattgca cgtctttgcc
    19141 gcgctcttta aaggccagac cgtggtcaga aaagtaaacc agcgagaagc tgctgccgct
    19201 gttgcgtaac tgatcgtaca gcttgcgcag taaatcgtcc gtttgcgtca tagtatagag
    19261 atagcacgac gtttctttcg attgcacaaa ggtttcgtat ttgccttttg tccggtcgca
    19321 ggcctgcgga tgcgagccca tcagatgcag aacaatcagc tgcggttgcg agtgctcttg
    19381 cgccagcact tgagcggtca tatccagtaa cgcttcgtct ttcgtgtttt tatctgcttc
    19441 aaaattacct tctttcagga agtacacttc atctgcacgt ttggcgatgc tggcgatagc
    19501 agtatcgtat tcgccgattt ggccctggtt ggaaaaccac caggtctgga agcccgcgcg
    19561 atttgccagg gtgacaaagt tatcctgaaa ctgtggtttg ccatcgacta cgcgattgag
    19621 cgttaagcca agcgatttct gcgtggagcc actggcggca atgtagtcag caaatatcaa
    19681 accgttaacg ctgctggcaa acggggtatt gtcccagtga ccgccaaagg caccgagggc
    19741 atcgcgacgc gcgctttcac cgatcaccac cacataggtc tgatacttcg gcttaacggc
    19801 agtgaccgtc caggtatctt tcattccgga aagttttgcc atccgttctt gctcttcaat
    19861 cacctcgtta ttattgacga tgacatcctt agcaaaacga aataccggat agccagtatc
    19921 tttcagttta aatacgccac cccaggccag gttttgcacg ggagcaacaa aaaatgtcgc
    19981 cacactgaat accagacaca ggctgtcgaa ggtattccag cgcgcttttt cattctcttt
    20041 ttttcgcctg attgctatta cgccgagcgc aaaaataaat aagccgacca ggtagctgta
    20101 ccacggaaaa atcgtcagga tttcggtcga ctcttccata ttggtggagt gcaatgccag
    20161 caatgtattg aaattaggcg cgccgtaggc ctgagcaaaa gggaaataac aagccgccac
    20221 cagcgaactg acaccgacca gaactttttg tacgcgaggc aatgttcgcc atagcaaaag
    20281 caaaatagcc gtaaacgcag cggtgtagag caaactgaag gggtaaccta agccgaggtt
    20341 aattaatagc gactgtaaaa agtagaacgc agtccacggg ctaaataccc ggctacgggt
    20401 aacaagcgat tctttgaggg ttaaattcat atgccactat cgtaacaaac gccatgtgct
    20461 taccctggcg tcaagggtca atacctgcaa gagtgcgtga agaggggaaa ggggtccgca
    20521 tccgcgagcc gcaatatacg cagggctgca agaagataga gcgagccgca gttcaggtca
    20581 actactgacg aacaaatctt ttgcgaaggg gattgcaaat ccgacaaaaa gtggggtttt
    20641 tcagaccaaa acgcgtcgtg cctgcatgaa agcaggagtg aagcggcgta ccgcgacgcc
    20701 gctatttatc ggaaggttta tcttgctgcg gtttgttgtt gaccatatcg cacaacatag
    20761 agagcagcat taaccgtact ttaaagggag aatgactaaa cacgcgcata cacctcttga
    20821 actcattcat aagacctcct gacttgctaa tcccgtcgat ccttgaggga tgattgcatt
    20881 acatacagat atagcacagg ctatattata tagctattgc taaaacgtta attttttgta
    20941 ccttcgcaac tctggtttac aatgtgcgca cgaaatgaga acgctatgta tgcgtcacca
    21001 taatgaggaa gcacaatgag tcgtcgcgca ggtacgccaa cagcaaaaaa agtgacgcag
    21061 ttagtgaacg aggaagagca cgttgaaggg ttccgccagg tcagagaggc gcatcggcgc
    21121 gagcttattg atgattacgt tgagctgatt tctgacttga tcagggaagt gggggaagct
    21181 cgtcaggtag acatggctgc tcgtttggga gtttcgcaac cgacggtggc taaaatgctt
    21241 aagcgactgg caactatggg gctgattgaa atgatcccct ggcgtggcgt gtttttaacg
    21301 gcagaaggag agaagctggc gcaggaaagc cgcgagcgac atcagatagt cgaaaatttc
    21361 ttgttggtgt tgggcgtcag tccggaaatc gcccgtcgcg acgcggaagg catggagcac
    21421 catgttagtg aagagacgct ggatgccttt cgtttgttta cccagaaaca cggtgccaaa
    21481 tgagcctgcc ttttttacgc acgctgcaag gcgatcgttt ttttcagtta ttaattcttg
    21541 ttggtatcgg attaagtttt ttcgtgccct ttgcaccgaa atcctggcct gctgctatcg
    21601 actggcacac catcatcacc ttaagcggcc tgatgctgct gaccaaaggt gtggagttaa
    21661 gcggttattt tgatgtgctg gggcgcaaaa tggtgcgccg ctttgctacg gagcgtcggc
    21721 tggcgatgtt tatggtgctg gcggcggcgc tgctttctac ctttctgacc aacgatgtca
    21781 cgctgtttat tgttgttccg ctgactatca cgctaaaaag gctgtgtgag atcccggtta
    21841 atcggctgat tatttttgag gcgctggcag tcaacgctgg ttcgctactg acgccaattg
    21901 gcaacccgca aaatattctt atctggggac gttctggtct ttcgtttgcc ggatttattg
    21961 cccaaatggc accgctggct ggcgcaatga tgctgacgct cctgctgttg tgctggtgtt
    22021 gtttccctgg aaaggcactc caataccata cgggggtgca aacaccggag tggaaaccgc
    22081 ggctggtgtg gagttgtctg gggctgtata tcgtctttct gacggcgctg gagttcaaac
    22141 aagagctgtg gggactggtg attgtggcgg cgggctttgc gctgctggcg cgtcgcgtgg
    22201 tgttgagtgt ggactggacg ctgctgctgg tgtttatggc gatgtttatc gacgtccatt
    22261 tactgaccca gcttccagcg ttgcaaggcg tgttgggtaa cgtgagtcat ttatctgaac
    22321 ccggattatg gttaacggca atcggtttat cgcaggtgat cagtaacgtg ccgagtacta
    22381 tattgttgct gaactatgtg ccgccgtctt tattactggc atgggcggta aacgtaggtg
    22441 gctttgggtt attacccgga tcgctggcaa atttgattgc gctacgtatg gcgaacgatc
    22501 gccgcatctg gtggcgtttc catctctatt caataccgat gctgttgtgg gcggcgctgg
    22561 tgggatatgt tttgttggtt atgatcccgg cctggtaagg tctggcaaat aaaaaaggcg
    22621 gattatgagt ccgcctttgt tttacgtttt gaagattaat tcagacgaac cggcatcccg
    22681 gagcggtttt taatcgcttc atcaagaaca acctggtcaa catctggctg gccggtcact
    22741 gtctgcacgc tcttcgtcag ggtaattggc acaatttcct gaccttcaaa ttgggcttcg
    22801 gtggtagaca gcgggttatg gacttcaatg taacggctgc cgtctggctc ggtggtcgct
    22861 tttaccggct catcaataaa ctgtacgcgg gtaccgaccg gtactttctc gaacaggaat
    22921 ttgatgtctt cgttacgcag acgcacacaa ccatgactta cacgcagacc gataccgaag
    22981 ttagcgttgg tgccatggat agcgtacagg cgaccgatgt agagtgcata cagccccatc
    23041 gggttatccg gacctgccgg aacgacagcc ggaagcggtt cgcccgcagc gcggtactct
    23101 gcgtgcattt tggcggtcgg cgtccaggtc gggcctgctt tcttacgctc aactttggtg
    23161 gtccagttga taggcgtatc tttgcctaac tgaccaatgc cgatcggcag cacgataacg
    23221 gtgttggtcc ctttcggata gtaatacaga cgcatctctg cactgttaat gacgatgcct
    23281 tcatgaacgg tatccggcag gatcagctgc tgcggaatgt tcagtacagt accgcctttc
    23341 ggcaggaagg tatccacacc cgggttcgct tccatcatat tggaaagccc catctggtac
    23401 tccgcggcaa aatactccag cggctgagtg ttaccttcag gaatggtgat cacctgattc
    23461 tgaccaacca ggcgactccc gtcggttggc agaggataag ttaccgcaga ggcggtactg
    23521 caaaagccga caacagcgaa ggccgctgcg aataatgttt tcaatttcat attcatgtta
    23581 agcgagattt tgtgcctggc aggccattgg gttgagaata ttagagtatt ggaagcgcat
    23641 tataagttca ttccagctca cagtgaaatc agatgtgtac gaaatcacat tttttgcctt
    23701 tggcttgagt gtagatctta agcgaggagc aggatcttct ttcagactta tggcataatg
    23761 cgcggtttgt catatctctt ttcaggatac gcctgtgtta gtttccagta acgtcaccat
    23821 gcagttcggc agtaagccgt tgtttgaaaa catttccgtc aaatttggcg gcggcaaccg
    23881 ttacggcctg attggcgcga acggtagtgg taaatccacc tttatgaaga tcctcggcgg
    23941 cgaccttgag ccgacgctgg gtaacgtttc cctcgatccc aacgagcgca ttggtaaact
    24001 gcgtcaggat cagtttgcct ttgaagagtt cactgtgctg gatacggtga tcatggggca
    24061 taaagagttg tgggaagtga agcaggagcg cgaccgcatc tatgctttgc cggaaatgag
    24121 tgaagaagac ggctataaag tggccgatct ggaagttaaa tacggcgaaa tggacggtta
    24181 ctctgcggaa gctcgtgccg gtgaattgtt gcttggcgtg ggaattccag tggaacagca
    24241 ctacggcccg atgagtgaag ttgctcctgg ctggaagctg cgtgtgttgc tggcgcaggc
    24301 gctgtttgct gatccggata ttctcctgct cgacgaaccg accaacaacc tcgacatcga
    24361 caccattcgc tggctggaac aggtgctgaa cgagcgtgac agcaccatga tcatcatctc
    24421 gcacgaccgt cacttcctta acatggtctg tacccacatg gcggatctgg attacggcga
    24481 gctgcgcgtt tatccgggta actacgatga gtacatgacg gcggcgaccc aggcgcgtga
    24541 acgtctgctg gccgataacg ccaagaagaa agcgcagatt gctgagctgc aatctttcgt
    24601 tagccgcttt agcgccaacg cctcgaaatc tcgccaggca acttcgcgcg cgcgccagat
    24661 tgataaaatc aaactggaag aggtgaaagc ctccagccgt cagaacccgt tcatccgttt
    24721 tgaacaggat aagaagctgt tccgtaacgc gctggaagtg gaaggtctga ccaaagggtt
    24781 tgataacggt ccgctgttta aaaatctcaa cctgctgctg gaagtcggtg aaaaactggc
    24841 ggtactgggt accaacggcg tcggtaaatc aacgctgttg aaaacgctgg tgggcgatct
    24901 gcaaccggac agcggcaccg taaaatggtc tgaaaacgcg cgcattggtt actatgctca
    24961 ggaccacgaa tatgagtttg aaaatgatct gaccgtgttc gaatggatga gccagtggaa
    25021 gcaggaaggc gatgacgagc aggcggtgcg cagtattctc ggtcgtttgc tgttcagcca
    25081 ggacgacatc aaaaagcctg ctaaagtgct ttccggtgga gaaaaagggc ggatgctttt
    25141 tggtaagtta atgatgcaga agccgaacat tctgatcatg gacgaaccga ccaaccacct
    25201 ggatatggaa tccatcgagt cactgaacat ggcgctggaa ctgtatcagg gcacgctgat
    25261 ctttgtttca cacgaccgtg agttcgtaag ctccctggcg acccgcattc tggaaatcac
    25321 cccggaacgc gtgatcgact ttagcggtaa ttacgaagat tacctgcgta gtaaagggat
    25381 cgagtaagtt attatccgtc accccgatac tgtcggggtg acggcggctg atgacaaacg
    25441 caagattgcc tgatgcgcta tgcttatcag gcattaagaa agcgcacttg tcagcaatct
    25501 gagtaacgtc gaatcgattt tcttaatccc acatcaattc acttcaatta actctcaaac
    25561 atccatgccc aacgctcgct taccgtgaat gttgaggtcg gcaagcgtaa agcgtccttc
    25621 ccagtttgtt tcatagtcct cgcgcgggaa gtcgcccggc gatgcgcctt tttccagtgc
    25681 ggccttcacc ttgtgcgcgt aggcaagatt tttctcgcac atcggtgccg caggaatgta
    25741 catcacgttg ccccaaccct gttgattttc aacgggggca acggaatgga tgacgtcgca
    25801 gtgccaccag acggagtctc cggcctcgag ttttggaatg ctggttaacg cctcaattaa
    25861 cagtggatgc cattgctctg ataccggcaa cactcttccg ggcgctacgc cgcacagttc
    25921 atcctccggc acatcatcaa gcagcggacg taacagtacg tacgccatgg cttcaggaat
    25981 gggcacgacg tgcagcaacc cctgactagg cagcatatca gagagcgctg tccagccctg
    26041 gaatgtccga aacacggaac atttggtggt gttgtccacc gtgtactctt caacttccgt
    26101 acgatgtgcc gcatgccagg gatcgtattt cgccagattg ccattaaaga cgttagcgaa
    26161 aacgtgctga tacgctggaa gcagccagcg ttccagcgcc ccggagtcgg tatgcgctcc
    26221 aagacctttg gaggtcgttc cgggcggacg gcggcggata cggtcagggt agatgacgct
    26281 cacatccggg ttaaaccatt gctttccatc actttcaaat gtccacagac gattgagaaa
    26341 cgactgcgca ttcgccattt cttcactctg gcgggcctgc atttgcgcct gcgaccagta
    26401 gatggggtaa atctcgggac gtgaagcgct gagcgtcccg aagaaattat cgccggggcc
    26461 tttgtagacc tcgtcaaagc ggttgcggtc cagatagtcc agcatcgact gatcccagcc
    26521 tagcgcttgt tcgcggggga aatggccttt tatcaccgca caaccgcgac gtttaatctg
    26581 ttcgcgctgc tctgcagtaa catgacctgc tttgatatcg gcataagaca gcaccggcca
    26641 gacggcatcg ccctgtgctt tgagtgcgtt gatttcagcc actcgcgtgg caatgtcatc
    26701 gcttagctga ttaaagatct gctggacgtc gccaagctgc gcccgcagcg cgtgcttcat
    26761 ctgacggata gctgctttgt gatcggcagg caatgtgtcg ctggtaaaag tagaagccat
    26821 aaccacctcg cattaacttt cattcgaaac tatgctgatt tgcatgtgac aatataagtt
    26881 ataaataagt taatgcaagt ttaatgattt gatgtgatgt acaggatgta aaaagaaggg
    26941 ataaccggag cgcatggctc cggtagagag gtcagctgtt aaaaggggat gtgttatcca
    27001 gtacagcctg aatcacattc agcgcgcctt catgattatt atcatcggta gcgtaacggg
    27061 cgatttgttt aatgttttcc gcagcattgc ccatcgcaaa ggaataacgc gccattttca
    27121 gcatctccgc atcgttaccg ctgtcgccaa tcgctaccac attttgcggt gacagatccc
    27181 agcgtttcag taaccgcgaa ataccgtttg ctttatgtag accgggaata atcaggtcga
    27241 taaagccaaa accactggtg acgggtttca taatgccatc gagcgctatg tgcagtttgt
    27301 cgatcactaa cgggatttgt tcatccggca ggttgagtga aaacttgaac agtacgtcgt
    27361 caatctcctg ataatctttt acaggtttca ggcgatggta gtgttttgcc atcagtgcga
    27421 caaatgcttc gggggcattt tcgctgacat atgcactttg cagaccgcag gcgacaaaat
    27481 tgagttgctt atcttttagc aactcgccaa taacaatccg cgattcatgt cgggtcagtt
    27541 cgccgtggaa cagttgcttg ccatgttcgt aaaccagtgc gccgttttcc gcgacaaaag
    27601 agatctcatc ctttagctca ggaaagaatg aaataagctg gtaatactga ttaccgctgg
    27661 cgacaacgaa ctcaatgccg cgctttttca gttcctgata ttgcgccata aaacgtggtt
    27721 ggttgtacgt tttgacgtcg ttaagaaaag taccgtccat atctgtgacg ataactttta
    27781 cgctcatagg tgtgctcccg gctcgaaaat gaaaccgtaa cagtgtaata acaatgtgac
    27841 gcagagcaca aattatattt cgaatgaaag taaggatgaa attgatgatg tgaatgattt
    27901 agcccggcga cgtctccgcc gggctgaggg gattacagca tatgttcggt acgggcgatg
    27961 atatcgtcct gagcgtctgg agagagcgcg gtgaagaagg cggaataacc cgcgacacgc
    28021 actaccagat cgcgatactg atcgggatgt tttttcgcct ccagcagcgt ttcgcgggaa
    28081 acgatgttgt actgaatatg ccagccttta tgcacttcaa agaaggtgcg cagcaggatc
    28141 atcagtttct gcttgtcaga ttcgttctcc agtgttgctg gattcagttt ctggttgagc
    28201 aacaccccgc cgagaatcgc tgccgtaggc agtttaccca ccgagccaat gaccgcagta
    28261 gggccaagat gatcagtacc ggaagccggg cttgcgcctt ctgccagcgg ggtgtgggct
    28321 ttacgtccgt ctggcgttgc catagtttgc gcgccaaacg gtacgttagc ggagatagac
    28381 gacgtacccg cgtaatagtt gccgccaacc ggaccacgac cgtagcgcgg gttatggtac
    28441 tgtttcagtt cgtcgatata ggtctgataa gcgcgagcca gcagcgtatc gacagtatca
    28501 tcgtcgttgc cgtacttcgg cgcaccgtta atcagccgct gacgcagctg ctcgtgagtc
    28561 aggccgtcga agtcattcgc cagtgcggca gccagctgtt gctgactaat cgcgccctgt
    28621 tcaaatacca gtttcttcac ggccgccagg ctgttgccga ggttggcaat gccaacctgc
    28681 aggccagaaa cccagtcata tttcgcgccg ccttgcttga tacttttcgc tcgctcaata
    28741 cagtcatcca ccagcgccga gcagagaata tcgtgcacgt tctcttccag catggtgtcg
    28801 acgacatatt cgatttcgat tgatttacgc gtgtagtaac ggatttgcgt atcccacgcg
    28861 tccatcactt catcgaagtt gttgaagtta cctgccgaca acgctttttc ttgtggcagg
    28921 aacactttgc cgctggtggc atcacgcccg ccttccagcg ccgccagcat cacgcgggcg
    28981 aagttgataa agctcatgcc ggtacagcga tagccccatt tgccaccgac ggcggtttcg
    29041 atacaaccaa tcgctgcata gttgtaagca tcctgcggtt caataccgag tttaataaat
    29101 tccgggatga cgatttcgtc gttgttgaac gccggcatcc cgaagccgca acggatcacc
    29161 tgtacgcagg cgtcgaggaa atcgttgctc attcccgcgt ggtaacgcac gctgaggtta
    29221 ggctgagtgg aacgcaggcg accgcaggat tcgaggatcg cgtaagagag tggattcacc
    29281 gcgtccattg gttgaccatc aaccagattt tgtccgccaa tggtgacgtt ctgatacaga
    29341 ggacttcccg cagaggcttt tgagtgtgag cctgagcgga ttttgttcac ttccagcagt
    29401 ttcagccagc agctatgcag caattcgatg gcgtgttcgc gatccagtgt ctggttgagt
    29461 tcaacgtcgc ggcgatagta cgggtagaga tactggtcca tacgaccaaa cgatactgag
    29521 tgaccgttag attcgatctg caaaatcaac tggatgaagt aacacagttg cagcgcctgc
    29581 cagaaagtct gcggcggctg gtgggcgata agatcgcagt tttctgccat cgccagcagt
    29641 tcatcgcgac ggctttcgcg ggtttcggtc gcggccattg cacgcgccag ggcagcgaaa
    29701 cgttcaatgt gttcactgac tgccaccagc acgatatcaa tcgctttcag gaactgttcg
    29761 ccgtgcagat cttccagtac cgtcagattg atgcgcgagc gacgttccgc tactttctcg
    29821 cgcagaccat caagcctttt tccagcagca gcgggaaatt caccgccagg tgcgcatcgc
    29881 cggaggtcat attgccttcc gctttaatga ttccggtcgc cagcagacct ttttgctcat
    29941 cggtaaacat gccgtagcag cgatcctgta ccgtctgacc gcgccaccac gggcacactt
    30001 catgcagaac gcgtttgttc tcttcgctca ccgcaaagcc agcaccggga cgatctgcca
    30061 gatcatcaat ctctttttcg atccacgaga cagtatattc cgggaagatc ggcgcggcgc
    30121 gaacttcgct tgcctggttg ccgatgatca actcatcgtg cttgatccag atagtgcgat
    30181 tcgccaggtg atgtgccagc gccagcgcgc gacgtacagg gatcggctta tcgagatgtt
    30241 gttgatacat ctcggtatag tgctgcgcgc gctcggtaca aaccggcggt ttcacaatat
    30301 gcaccagcgc atttttgtgc gctttaatgc ggtcgctgag cgtgtccagt ttcagtgtgg
    30361 tcatggttgt tatcctcgta aggtcgcggt taaccctttc tggcaggcat actgctgggc
    30421 aaagtcgagc agttctggcg catcaagcgg tttttccggg gcgtcatagg gcagattaag
    30481 taagtgatat ttgttgatgc ccagcgtgtg gtagggcagg aaatgaattt cgccaacgtg
    30541 cagctcgtcg gcggcaaaat cggtaatggc tttcacagag gtttcgtcgg cattaaagcc
    30601 ttgaatcagc ggcacgcgga taatgatttt tttgcccgct gcggcgagtt ttttcaggtt
    30661 atccagcacc ctggcggcgt taccgtcggt ccactgttta aacggcgcgt cggcaacgtg
    30721 ttttaaatcg gcaagaaaca gatcgatata gggcagagaa ggggcgatat atttccacgg
    30781 cacatgcagg caggtttcta ccgcagtatg aatgccggcc tcgtggctgg cttgcagtag
    30841 cgccgtcgcc atttccggct gcatgaatgg ttcaccgcca gaaagtgtta agccgccgcc
    30901 gctgcgatcg taaaacggtt tatcgcgcag aacggtcacc atgatctcct caacgctttt
    30961 cacttcccca cacacggtta acgactgtgt cgggcagcag tcggttaacg ccgtcagatg
    31021 ctccggggtt aacttttccc gatgaataag caaaccattc agcgcgcgct caatcacttc
    31081 cggcgcggtc ttagcgcaca gctcacagcc ttccaggcac agacgtgggt catacagcag
    31141 atcctgcgtg cgggcgcggc tttccgggtt ctgacaccag cggcagccca gcgaacagcc
    31201 tttaagaaat acgaccgtgc ggataccggg gccatcatgg gtcgagtagc gctgaatatt
    31261 gaaaatcata gttgcctctc tgtttcgttc aaatattaaa atactttcga atgaaagtta
    31321 gattgatgtg cgtcaactgt tcagagagtt ttcccgtgat agtctacatt cagacaaaaa
    31381 gtacattttg aggatggtta tggaactgta tctggatact tcagacgttg ttgcggtgaa
    31441 ggcgctgtca cgtatttttc cgctggcggg tgtgaccact aacccaagca ttatcgccgc
    31501 gggtaaaaaa ccgctggagg ttgtgcttcc ggaacttcat gaagcgatgg gcggtcaggg
    31561 gcgtctgttt gcccaggtaa tggctaccac tgctgaaggg atggtgaatg acgcacgtaa
    31621 attgcgttct attattgcgg atatcgtggt gaaagttccg gtgaccaccg aggggctggc
    31681 agctattaag atgttaaaag cggaagggat tccgacgctg ggaaccgcgg tatatggcgc
    31741 agcacaaggg ctgctgtcgg cgctggcagg tgcggaatat gttgcgcctt acgttaatcg
    31801 tattgatgct cagggcggta gcggtattca gactgtgacc gacttacacc agttattgaa
    31861 aatgcatgcg ccgcgggcga aagtgttagc cgcgagtttc aaaaccccgc gtcaggcgct
    31921 ggactgctta ctggcaggat gtgaatcaat tactctgcca ctggatgtgg cacaacagat
    31981 gattagctat ccggcggttg atgccgctgt ggcgaagttt gagcaggact ggcagggagc
    32041 gtttggcaga acgtcgattt aaccggacgt tctgcatcct cataaattgc tgatgacgtg
    32101 gcggagtgca ccgatgcctg ccgcgcttgg aaatctgtgc ccgaaccgta ggccagataa
    32161 ggcgttcacg ccgcatctgg cgtctggagc aaatgcctga tgcgacgctt gtcgcgtctt
    32221 atcaggcctg gaggtggcaa ttactgcccg cacacctcac accccggatt gcgcatcagt
    32281 ttcatttcgc gaaactgaca ggtcatcgca tcgtacatga cgattttccc gctggccggt
    32341 ttgccataac ctgccagcag tttgatcgct tccattgcct gcaacgaacc aatcacgccg
    32401 atcaacggtg ccatcacgcc tgcttctacg caggttaacg cgttctcgct aaacaaacgg
    32461 ctaaggcagc gatagcacgg ttcgccgtcc tgataagtga agaccgtgat ttgcccttcc
    32521 atacgaattg ccgcgccgga aaccagcggc accttcgcgg caaaacagcc tgcgttcagt
    32581 tgattacgta ccgcaacgtt atccgtacag tcgagcacaa gattgtgttc agcaatcaat
    32641 gctgcaagtt ctgcgtcatc cagcagtgca ttgactggcg taatcgcgat atgtgggttg
    32701 atccgcgtca gagcgtcacg ggcggattcc accttcggtt gcccgaccgt agcatcactg
    32761 tgcagcgtct ggcgttgcag attcgagagc gaaaccgagt cgaagtcgag cagcgtcagg
    32821 ttgccgacac cggcgcttgc cagatactgc gaagctgcgc agccgagtcc accgaggccg
    32881 acgaccagca cacgagaatc tttcagcgcc tcctgaccgt caaaatcaaa accgcgcaga
    32941 atgatttgcc ggttgtagcg cagcatctct tgatcgctga gttccgccat tacaggcctc
    33001 cgaacaacgc gttaaacggt tctacttcca cccattcgcc cacttccaca ttgccgcgat
    33061 cgcgttccag cacgataaag cagttgccga ggctaaagga gctgaatata tgtgaaccct
    33121 gatgtccggt ggtcgtcact tccagttcgc catcggcgtt gcggtgcagc acgccgcgct
    33181 ggaaatcaag acgtcctggc gtttttttca ggcgggacgc tgtgcgtacg cgctggcgcg
    33241 cgggcaggcc gctggcggtg ttgccgctta gttttgccag caaaggctgt accagttgat
    33301 agaaggtcag cgtcgctgaa accgggttgc ccggcaggcc gcagaaccag ctattgctga
    33361 gtttaccgaa cgcgaacggt ttacctggtt taatcgccag cttccagaag gcgatctccc
    33421 ccagctcttc aagaatcgtt ttggtgtaat ccgcttcacc cactgaaaca ccgccggaac
    33481 tgatcaccac atccgcctgg ctgtcggctt caataaatgc ggcgcgcagg gcatgtggat
    33541 cgtcgcggat aatccctaag ttgattacct cgcaacccag ctgttctaac atcaggtgta
    33601 cggcgagacg gttggtatcg tagatttggc cgtcgcccag cggctggcct ggcaactgga
    33661 gttcatcacc ggtagaaaaa agcgctacac gcactttacg aatcaccgga acttcggcaa
    33721 tccccagtga agcaattact ggcagctctg cggtagtcag gcgagtcccc gccgggaaaa
    33781 caaccgcacc tgcagagata tcttcaccgc gacggcgaat attttgcccg ctacgcactt
    33841 cagcagtaaa acgcacgcca ttgtccgttt gttcagtctg ctcctgcatc accaccgctt
    33901 cgcagccttc cggcaccggc gcaccggtca taatacgaat gcaggtaccc gcaggccatt
    33961 caccatggta cggctgaccg gcaaaggatt taccggcaac gggcagcggt tgtccggagg
    34021 tgatatcggc caaacgtacc gcgtaaccat ccattgcgga gttatcaaac cctgggacat
    34081 ccagcggcga aacgacatcg ctcgccagaa tacgaccaaa acactgaacc agtggcagcg
    34141 tttcctgggc ggtcagtggg gtgacgcgag aaagcatctc attaagcgcg gtgtcgagcg
    34201 acatcaatcc ggtggtaaat tccatgaaaa cactcctgcg gaggcaaaat cgaatttgcc
    34261 tattatgtca gaaaaacgcc acagactgta tgccacctcg ggcgtagcgc tgggtcctgc
    34321 ctttacatgc catatccatc tttctatatt caaaaattga atgagtaatt cataaaaatt
    34381 ctgatattta tagcaaaagt ggcgagccac ccttaatgga cgaatactat gggcaaagca
    34441 gtcattgcaa ttcatggtgg cgcaggtgca attagccgcg cgcagatgag tctgcaacag
    34501 gaattacgct acatcgaggc gttgtctgcc attgttgaaa ccgggcagaa aatgctggaa
    34561 gcgggcgaaa gtgcgctgga tgtggtgacg gaagcggtgc gtctgctgga agagtgtcca
    34621 ctgtttaacg ccggaattgg cgccgtcttt acgcgtgatg aaacgcatga actggacgcc
    34681 tgtgtgatgg atggtaacac cctgaaagcc ggtgcggtgg cgggcgttag tcatctgcgt
    34741 aatccggttc ttgccgcccg gctggtgatg gagtaaagcc cgcatgtgat gatgattggc
    34801 gaaggggcag aaaattttgc gtttgctcat ggcatggagc gcgtctcacc ggagattttc
    34861 tccacgcctt tgcgttatga acaactaatg gcagcgcgcg aggaaggggc aacagtcctc
    34921 gaccatagcg gtgcgccact ggatgaaaaa cagaaaatgg gcaccgtggg ggccgtggcg
    34981 ttggatttag acggcaatct ggcggcagcc acgtccacgg gcggaatgac caataaatta
    35041 cccggacgag ttggcgatag ccccttagtg ggtgccggat gctacgccaa taacgccagt
    35101 gtggcggttt cttgtaccgg cacgggcgaa gtcttcatcc gcgcgctggc ggcatatgac
    35161 atcgccgcgt taatggatta cggcggatta agtctcgcgg aagcctgcga gcgggtagta
    35221 atggaaaaac tccctgcgct tggcggtagc ggtggcttaa tcgctatcga ccatgaaggg
    35281 aatgtcgcgc taccgtttaa caccgaagga atgtatcgcg cctggggcta cgcaggcgat
    35341 acgccaacca ccggtatcta ccgtgaaaaa ggggacaccg ttgccacaca gtgatgaact
    35401 tgatgccggt aatgtgctgg cggttgaaaa tcttaatatt gcctttatgc aggaccagca
    35461 gaaaatagct gcggtccgca atctctcttt tagtctgcaa cgcggtgaga cgctggcaat
    35521 tgttggcgaa tccggctccg gtaagtcagt gactgcgctg gcattgatgc gtctgttgga
    35581 acaggcgggc ggtttagtgc agtgcgataa aatgctgttg cggcggcgca gtcgtgatgt
    35641 gattgaactt agcgagcaga gcgctgcaca aatgcgccat gtgcgcggtg cggatatggc
    35701 gatgatattt caggagccga tgacatcgct gaacccagta tttactgtgg gtgaacagat
    35761 tgccgaatca attcgtctgc atcagaacgc cagtcgtgaa gaagcgatgg tcgaggcgaa
    35821 gcggatgctg gatcaggtac gcattccgga ggcacaaacc attctttcac gttatccgca
    35881 tcaactctct ggcgggatgc gccagcgagt gatgattgcg atggcgctgt catgccgccc
    35941 ggcagtgctg atagccgatg agccaaccac cgcgctggat gtcactattc aggcgcagat
    36001 cctgcaatta atcaaagtat tgcaaaaaga gatgtcgatg ggcgttatct ttatcactca
    36061 cgatatgggc gtggtggcag agattgccga tcgggtactg gtgatgtatc agggcgaggc
    36121 ggtggaaacg ggtaccgtcg aacagatttt tcatgcaccg caacatccct acacccgtgc
    36181 gctgttagct gctgttccgc aacttggtgc gatgaaaggg ttagattatc cccgacgttt
    36241 cccattgata tcgcttgaac atccagcgaa acaggccccc cccatcgagc agaaaacggt
    36301 ggtggatggc gaacctgttt tacgggtgcg taatctggtc acccgtttcc ctttgcgcag
    36361 cggtttgttg aatcgcgtaa cgcgggaagt gcatgccgtt gagaaagtca gttttgatct
    36421 ctggcctggc gaaacgctat cgctggtggg cgagtctggc agcggtaaat ccactaccgg
    36481 gcgggcgttg ctgcgcctgg tcgaatcgca gggcggcgaa attatcttta acggtcagcg
    36541 aatcgatacc ttgtcacccg gcaaacttca ggcattgcgc cgcgatattc agtttatttt
    36601 tcaggaccct tacgcttcgc tggacccacg tcagaccatc ggtgattcga ttatcgaacc
    36661 gctgcgcgta cacggtttat tgccaggtaa agaagcggtt gcacgcgttg cgtggttgct
    36721 ggagcgcgtg ggcctgttac ctgaacatgc ctggcgttac ccgcatgagt tttccggcgg
    36781 tcagcgccag cgcatctgca ttgctcgcgc gttggcattg aatccaaaag tgatcattgc
    36841 cgacgaagcc gtttcggcgc tggatgtttc aattcgcggg cagattatca acttgttgct
    36901 cgatctccag cgtgatttcg gcattgcgta tctgtttatc tcccacgata tggctgtggt
    36961 agagcggatt agtcatcgtg tggcggtgat gtatctcggg caaattgttg aaattggtcc
    37021 acggcgcgcg gtcttcgaaa acccgcagca tccttatacg cgtaaattac tggcggcagt
    37081 tccggtcgct gaaccgtccc gacaacgacc gcagcgtgta ctgctgtcgg acgatcttcc
    37141 cagcaatatt catctgcgtg gcgaagaggt ggcagccgtc tcgttgcaat gcgtcgggcc
    37201 ggggcattac gtcgcacaac cacaatcaga atacgcattc atgcgtagat aacattcagg
    37261 cggagaataa aatggcaaga gctgtacacc gtagtgggtt agtggcgctg ggcattgcga
    37321 cagcgttgat ggcatcttgt gcattcgctg ccaaagatgt ggtggtggcg gtaggatcga
    37381 atttcaccac gctcgatccg tatgacgcga atgacacgtt atctcaggcc gtagcgaaat
    37441 cgttttacca ggggctgttc ggtctggata aagagatgaa actgaaaaac gtgctggcgg
    37501 agagttatac cgtttccgat gacggcatta cttacaccgt gaaattgcgg gaaggaatta
    37561 aattccagga tggcaccgat ttcaacgcag cggcggtaaa agcgaatctg gaccgggcca
    37621 gcgatccggc gaatcatctt aaacgctata acctgtataa gaatattgct aaaacggaag
    37681 cgatcgatcc gacaacggta aagattaccc tcaaacagcc gttctcagcg tttattaata
    37741 ttcttgccca tccggcgacc gcgatgattt caccggcagc gctggaaaaa tatggcaagg
    37801 agattggttt tcatccggtg ggaaccggac cgtatgaact ggatacctgg aatcagaccg
    37861 attttgtgaa ggtgaaaaaa ttcgcgggtt actggcagcc aggattgccc aaactggaca
    37921 gcataacctg gcgtccggtg gcggataaca acacccgcgc ggcaatgctg caaaccggtg
    37981 aagcgcagtt tgctttcccc attccttacg agcaggccgc actgctggag aaaaacaaaa
    38041 atatcgagtt gatggccagt ccgtcaatta tgcagcgtta tatcagtatg aacgtgacgc
    38101 aaaagccatt cgataacccg aaggtccgtg aggcgctgaa ttacgccatt aaccgtccgg
    38161 cgctggtgaa agtggccttc gcgggctatg caacgccagc tactggtgtg gtaccgccga
    38221 gtatcgccta cgcgcaaagt tataaaccgt ggccttacga tccagtgaaa gcgcgcgaat
    38281 tactgaaaga ggcgggatat cccaacggtt tcagtaccac gctgtggtcg tcacataacc
    38341 acagcaccgc gcagaaagtg ctgcaattta cccagcagca gttagcgcag gtcgggatta
    38401 aagcccaggt gactgcgatg gatgccggac agcgggcggc agaagttgaa ggtaaagggc
    38461 aaaaagagag cggcgtgcgg atgttctaca ctggctggtc ggcttcaacc ggcgaagcgg
    38521 actgggcact atcgccgttg tttgcctcgc aaaactggcc accgacgctg tttaataccg
    38581 cgttttacag caataaacag gtggatgact tcctggctca ggcactgaaa actaatgatc
    38641 cggcggaaaa gacccgctta tataaggcgg cgcaggatat catctggcaa gagtcgccgt
    38701 ggatcccgct ggtggtagaa aaactggtgt cggcacacag taaaaacctg accggttttt
    38761 ggatcatgcc agataccggc ttcagctttg aagacgcgga tttgcaataa gcaacgcagg
    38821 gagtggaatg cttaattacg ttatcaaacg attactgggg ttgattccga cgctgtttat
    38881 cgtcttggtg ctggtgtttt tatttgtgca tatgctgccc ggcgatccgg cgcgattgat
    38941 tgccgggccc gaagctgatg cgcaggttat agaactggtg cgtcagcagc tggggttgga
    39001 tcagccgctg tatcaccagt tctggcacta tatcagcaat gctgtgcagg gggattttgg
    39061 cctgtcgatg gtgtcacgtc gtccggttgc cgatgagatt gccaggcgct ttatgccaac
    39121 gctgtggctg accataacca gtatggtctg ggcggtgata tttggtatgg cggcgggaat
    39181 tatcgccgcc gtctggcgta accgttggcc ggatcgattg agtatgacca ttgcggtgtc
    39241 ggggatctcg tttccggcat ttgcgctggg gatgctttta attcaggtat tctccgttga
    39301 actgggctgg ctgcctaccg tgggagcaga cagttggcag cactacattt taccctccct
    39361 gacgctcggc gcggcagtgg ccgccgtgat ggcgcgcttt acccgcgcgt cgtttgtcga
    39421 cgttttaagc gaagattata tgcgtaccgc gagggcgaaa ggggtgagcg aaacctgggt
    39481 tgtcctcaaa cacgggctac gcaacgcgat gatcccggta gtgaccatga tgggcttaca
    39541 gtttggcttt ttgctcggtg gttccatcgt ggtggagaaa gttttcaact ggcccggact
    39601 tggacgctta ctcgttgact ccgtagaaat gcgcgattac ccggtgattc aggcggaaat
    39661 tctgcttttc tcgctggaat ttattcttat caacttagtg gtggatgtgc tttacgccgc
    39721 cattaacccg gctatcaggt acaagtaagg atgcgactat ttaactggcg acgtcaggcg
    39781 gtgttaaacg ccatgccact ggtcaaacct gaccaggtac gtacaccgtg gcatgaattc
    39841 tggcgacgat ttcgccgtca gcatatggcg atgaccgccg cattattcgt tattttattg
    39901 attgtggtgg ccatttttgc acgctggatc gctccctatg acgccgaaaa ttattttgat
    39961 tatgacaatc tgaataacgg accttctttg cagcactggt ttggcgtcga ttcactgggg
    40021 cgcgacattt tcagccgtgt cctggttggt gcgcaaatct cgctggcggc gggcgtgttt
    40081 gccgtgttta tcggtgcggc gatagggacg ttgctgggct tgttggctgg atattatgaa
    40141 ggctggtggg atcggctgat catgcgcatt tgcgatgtgc tgtttgcctt cccgggtatt
    40201 ttactggcga tcgctgttgt tgcggtgttg ggaagcggca ttgctaacgt gattattgca
    40261 gtcgccattt tttccatccc tgcgtttgcc cgcctggtgc gcggcaacac gctggtgttg
    40321 aaacagcaaa cctttattga gtcagcacgc agtattggtg ccagcgatat gaccattttg
    40381 ttgcgtcata tcctgcctgg gaccgtctct tctatcgtgg tgtttttcac catgcgcatt
    40441 ggtacctcga ttatctctgc cgccagcctg tcatttctcg gcctcggtgc gcagccgccg
    40501 acaccagagt ggggagcaat gctcaatgag gctcgagcgg atatggttat cgcgccgcat
    40561 gtcgctgttt ttccggccct ggctattttt ctgactgtac tggcgttcaa tttgttgggc
    40621 gatggtttac gcgatgcgct ggatccgaaa attaaaggat agttacgttt gaatattgct
    40681 tgaaagagta atcacctcac aggaaattat tgccctaagc aagtgttgta actttctgct
    40741 gattttgtag aatcgggtaa tttggttaaa tagccgcagc aagggactgt ttttgcagcg
    40801 gc
//
LOCUS       DAZKIP010000026        40662 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZKIP010000026 DAZKIP010000000
VERSION     DAZKIP010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.026"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..135
                     /locus_tag="ACHOUK_001663"
     CDS             <1..135
                     /locus_tag="ACHOUK_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9897133.1"
                     /translation="KIQDKIDELSKNLFNTMTETIENNFDDIFRQQSENMSNYYEFVD
                     "
     gene            complement(313..828)
                     /gene="pgpA"
                     /locus_tag="ACHOUK_001664"
     CDS             complement(313..828)
                     /gene="pgpA"
                     /locus_tag="ACHOUK_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFP9897134.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGIL"
     gene            complement(806..1783)
                     /gene="thiL"
                     /locus_tag="ACHOUK_001665"
     CDS             complement(806..1783)
                     /gene="thiL"
                     /locus_tag="ACHOUK_001665"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFP9897135.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVVPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            complement(1861..2280)
                     /gene="nusB"
                     /locus_tag="ACHOUK_001666"
     CDS             complement(1861..2280)
                     /gene="nusB"
                     /locus_tag="ACHOUK_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFP9897136.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(2300..2770)
                     /gene="ribE"
                     /locus_tag="ACHOUK_001667"
     CDS             complement(2300..2770)
                     /gene="ribE"
                     /locus_tag="ACHOUK_001667"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFP9897137.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(2859..3962)
                     /gene="ribD"
                     /locus_tag="ACHOUK_001668"
     CDS             complement(2859..3962)
                     /gene="ribD"
                     /locus_tag="ACHOUK_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFP9897138.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDVQTQAL
                     YPQHHLRQPVRIVIDSQNRVTPEHHIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(3966..4415)
                     /gene="nrdR"
                     /locus_tag="ACHOUK_001669"
     CDS             complement(3966..4415)
                     /gene="nrdR"
                     /locus_tag="ACHOUK_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFP9897139.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            4566..5105
                     /gene="yajI"
                     /locus_tag="ACHOUK_001670"
     CDS             4566..5105
                     /gene="yajI"
                     /locus_tag="ACHOUK_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFP9897140.1"
                     /translation="MNINVFRLILLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            5404..6288
                     /gene="tsx"
                     /locus_tag="ACHOUK_001671"
     CDS             5404..6288
                     /gene="tsx"
                     /locus_tag="ACHOUK_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFP9897141.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(6465..6812)
                     /gene="yajD"
                     /locus_tag="ACHOUK_001672"
     CDS             complement(6465..6812)
                     /gene="yajD"
                     /locus_tag="ACHOUK_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFP9897142.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(6900..7181)
                     /locus_tag="ACHOUK_001673"
     CDS             complement(6900..7181)
                     /locus_tag="ACHOUK_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9897143.1"
                     /translation="MYKLSGKAVEDFRGIYDYTLGKFGDEQADRYTDSLGTFLDTLSQ
                     MPEIGQDYDAIPEVKKIAFRFHTVYYVIRVDDILIARILHQLMEPRRHW"
     gene            complement(7174..7416)
                     /locus_tag="ACHOUK_001674"
     CDS             complement(7174..7416)
                     /locus_tag="ACHOUK_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFP9897144.1"
                     /translation="MRKITSVSVGEQLDSFITRMVQSGRYGSASEVMRSALRLLEQQE
                     SRDEAVRNAVIEGLESGESSMTLRDIAAERKQKHRV"
     gene            complement(7568..8539)
                     /gene="secF"
                     /locus_tag="ACHOUK_001675"
     CDS             complement(7568..8539)
                     /gene="secF"
                     /locus_tag="ACHOUK_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFP9897145.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(8550..10397)
                     /gene="secD"
                     /locus_tag="ACHOUK_001676"
     CDS             complement(8550..10397)
                     /gene="secD"
                     /locus_tag="ACHOUK_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFP9897146.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(10425..10757)
                     /gene="yajC"
                     /locus_tag="ACHOUK_001677"
     CDS             complement(10425..10757)
                     /gene="yajC"
                     /locus_tag="ACHOUK_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFP9897147.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(10780..11907)
                     /gene="tgt"
                     /locus_tag="ACHOUK_001678"
     CDS             complement(10780..11907)
                     /gene="tgt"
                     /locus_tag="ACHOUK_001678"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFP9897148.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(11962..13032)
                     /gene="queA"
                     /locus_tag="ACHOUK_001679"
     CDS             complement(11962..13032)
                     /gene="queA"
                     /locus_tag="ACHOUK_001679"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFP9897149.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            13125..13706
                     /gene="acpH"
                     /locus_tag="ACHOUK_001680"
     CDS             13125..13706
                     /gene="acpH"
                     /locus_tag="ACHOUK_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFP9897150.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYAALETRFWQFYPRMMAQASRKAL"
     gene            complement(13727..15172)
                     /locus_tag="ACHOUK_001681"
     CDS             complement(13727..15172)
                     /locus_tag="ACHOUK_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9897151.1"
                     /translation="MTISNSNLLKPFITGTELPPFKEDVFSKTEEQFKKSTYGKSLFI
                     SGNENLVNISAEKIDEIKNSKNFFSNIVNIIILLIKHPDKILPILHGCPVESIEVIKN
                     AKRTGQWSYEMTDKNGQSIEVSYIFTDTIDDKSDGKLCLKICNSCNQNDSSTHEYKID
                     HQQLKNHIRQLEEWALLEKFYDNNAYYSKRTKQLEKKIADAGFITRKVKAENFNVKEF
                     KDDIASMLCEQPPMRTHQISSSGYVSADKLHKLYKTNLEDAQRDLKTTPVDIDGVSLK
                     SITREKIAEEIAKKTAEIIAKQNAKSIEEKNIEKNKKQTNSEDNFVLKFSPEEINEML
                     LDYSDEEQKKLRPFFTSDHIYCLFDAFSQGLHNVARNGSPRYAGLLRQIASKSAILQD
                     FDQQLFWRTFTGGVLHRHISVKSEPEKTDVWRFSSQFDFPHINENGELSRTVLMFEGV
                     AESNFKDIANHSSECFSYRCYAAPLDANAKK"
     gene            complement(15360..17177)
                     /gene="malZ"
                     /locus_tag="ACHOUK_001682"
     CDS             complement(15360..17177)
                     /gene="malZ"
                     /locus_tag="ACHOUK_001682"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFP9897152.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNLQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGREVARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     MALFALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPFLNVVQWQRKEGHGQLTDGILALPAISATVWMN"
     gene            complement(17333..18706)
                     /gene="proY"
                     /locus_tag="ACHOUK_001683"
     CDS             complement(17333..18706)
                     /gene="proY"
                     /locus_tag="ACHOUK_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFP9897153.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(18782..20101)
                     /gene="brnQ"
                     /locus_tag="ACHOUK_001684"
     CDS             complement(18782..20101)
                     /gene="brnQ"
                     /locus_tag="ACHOUK_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFP9897154.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(20508..21803)
                     /gene="phoR"
                     /locus_tag="ACHOUK_001685"
     CDS             complement(20508..21803)
                     /gene="phoR"
                     /locus_tag="ACHOUK_001685"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFP9897155.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSVISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIVKNSD"
     gene            complement(21861..22550)
                     /gene="phoB"
                     /locus_tag="ACHOUK_001686"
     CDS             complement(21861..22550)
                     /gene="phoB"
                     /locus_tag="ACHOUK_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFP9897156.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            22740..23942
                     /gene="sbcD"
                     /locus_tag="ACHOUK_001687"
     CDS             22740..23942
                     /gene="sbcD"
                     /locus_tag="ACHOUK_001687"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFP9897157.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            23939..27082
                     /gene="sbcC"
                     /locus_tag="ACHOUK_001688"
     CDS             23939..27082
                     /gene="sbcC"
                     /locus_tag="ACHOUK_001688"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFP9897158.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQNTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLMLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLLQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            27208..28392
                     /gene="araJ"
                     /locus_tag="ACHOUK_001689"
     CDS             27208..28392
                     /gene="araJ"
                     /locus_tag="ACHOUK_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFP9897159.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(28535..29443)
                     /gene="mak"
                     /locus_tag="ACHOUK_001690"
     CDS             complement(28535..29443)
                     /gene="mak"
                     /locus_tag="ACHOUK_001690"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFP9897160.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            29568..30479
                     /gene="rdgC"
                     /locus_tag="ACHOUK_001691"
     CDS             29568..30479
                     /gene="rdgC"
                     /locus_tag="ACHOUK_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFP9897161.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMANPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(30564..31013)
                     /locus_tag="ACHOUK_001692"
     CDS             complement(30564..31013)
                     /locus_tag="ACHOUK_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9897162.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNGGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKNYCPP"
     gene            complement(31010..33289)
                     /locus_tag="ACHOUK_001693"
     CDS             complement(31010..33289)
                     /locus_tag="ACHOUK_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HFP9897163.1"
                     /translation="MNRINILVICMVLFFMTGNACATEWISSEDLITSDFHLMTADER
                     NVVKAATDDSMEAAYMLKDNIRWYYHNGDLSLPANFSNKNKLVVNGNLTISGDYDDYL
                     SGNGHLIVLGNVIVDNFINHDFAYVKGQMTAKGLVYADYNDHNFEVMKGISARGIIVS
                     DKATQFEVIKAEFYINEDGSGEGYNWDENIQKAYSLVTADLYDHTEIETDNISNAYPD
                     YDSVADNIVQGLPLFRDKAAPEINEKLKWIETGKLDNFPANKIKHQDPLVARFLTHTE
                     SLSPAVMLQLLQHPDDQTRESMAQSWPAQQMHWLTDELIKDEAVARGLVKNSNISADV
                     NKKLMSVPVESVQLEQARQDNLSPDIVASLSHSPFLSVRKTLLSHYDYAWLVPTAVAD
                     ELINNEDPELRERITGADLTAQQAVMLSKDKSLKVREALARTLTELKITQLSATLRTE
                     DIERIAEQMYLDNKENKNIVKVLLIALPEMRQLSLAKEDVHNLREGARYLTSKDVISY
                     LLTQHDVPTVWDELARDKLLPLEYKKQLWQRTLNLMMSKRQEDQEQAYEVQLALIDNG
                     VVDEEMLNNAIDLLVDLPAEYRYRMRNQLFDNKELPSGIINKLDQQYRFNSDWALAVV
                     SMKNSTRRQSERGLHRWNHEDSDIFAELATIKDKSDDEWWRALLQSRNDHLRQTALRN
                     AHTPASLLTTLTESQDRSLAINNPQLAADVKTVWLKEDPSLLLFVDKPDLSQLRDLVK
                     TGATRKIRNEARHRLEEKQ"
     gene            complement(33433..33717)
                     /gene="ppnP"
                     /locus_tag="ACHOUK_001694"
     CDS             complement(33433..33717)
                     /gene="ppnP"
                     /locus_tag="ACHOUK_001694"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFP9897164.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(33789..34466)
                     /gene="aroM"
                     /locus_tag="ACHOUK_001695"
     CDS             complement(33789..34466)
                     /gene="aroM"
                     /locus_tag="ACHOUK_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFP9897165.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNHLAHVSRRKVERDLQGVVEVLDNQGYDVIILMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEELLTVQAQKWQILQKPPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFNQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(34724..34915)
                     /gene="yaiA"
                     /locus_tag="ACHOUK_001696"
     CDS             complement(34724..34915)
                     /gene="yaiA"
                     /locus_tag="ACHOUK_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFP9897166.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(34965..35489)
                     /gene="aroL"
                     /locus_tag="ACHOUK_001697"
     CDS             complement(34965..35489)
                     /gene="aroL"
                     /locus_tag="ACHOUK_001697"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFP9897167.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(35672..36130)
                     /gene="yaiI"
                     /locus_tag="ACHOUK_001698"
     CDS             complement(35672..36130)
                     /gene="yaiI"
                     /locus_tag="ACHOUK_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFP9897168.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            36250..37059
                     /gene="proC"
                     /locus_tag="ACHOUK_001699"
     CDS             36250..37059
                     /gene="proC"
                     /locus_tag="ACHOUK_001699"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308464.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFP9897169.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDKFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(37076..38191)
                     /gene="adrA"
                     /locus_tag="ACHOUK_001700"
                     /gene_synonym="dgcC"
     CDS             complement(37076..38191)
                     /gene="adrA"
                     /locus_tag="ACHOUK_001700"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFP9897170.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGIWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVNLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(38293..38613)
                     /gene="psiF"
                     /locus_tag="ACHOUK_001701"
     CDS             complement(38293..38613)
                     /gene="psiF"
                     /locus_tag="ACHOUK_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFP9897171.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(38732..40147)
                     /gene="phoA"
                     /locus_tag="ACHOUK_001702"
     CDS             complement(38732..40147)
                     /gene="phoA"
                     /locus_tag="ACHOUK_001702"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFP9897172.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTADQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(40248..40508)
                     /gene="iraP"
                     /locus_tag="ACHOUK_001703"
     CDS             complement(40248..40508)
                     /gene="iraP"
                     /locus_tag="ACHOUK_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFP9897173.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT        10222 a        10743 c         9785 g         9912 t
ORIGIN      
        1 aagattcaag acaaaataga tgaactttct aaaaatttat tcaacactat gaccgaaacc
       61 attgagaata attttgatga tatttttcgt caacagtcag agaatatgag caattattat
      121 gagttcgtcg actgacgata tttcagtgtc attgaattat gagtgacaaa gatacacctt
      181 gaaatatcgc cgcatagtat attcggcgat gtcgggtttt actttaacag agtgccccaa
      241 aattggggca ctgttcattt ataaatcaaa ccgggtggtc tggaatcgcc tacacctccc
      301 ggtcaatggc atttaaagaa tacccagcgg ccagtgatga ccgataaagt acaggatgcc
      361 tgcggaaatc accccggcga caatatcatc gatcatgatc cccatgccgc catgtacatt
      421 gcgatcaaac cagcggatcg gccacggctt ccacatatcc agaatacgga aaatcacaaa
      481 cccggcggcg acccactgcc agtcattggt cggcagcgcc atcagggtga tccacatgcc
      541 aataaactca tcccatacga tgctaccgtg atcgtgcacc cccatgtctt tcgccgtttg
      601 atgacaaaga tagacgccga tacagatccc caacatcacc accagcgagt agagctgcca
      661 gggcaaaaag gtcatcagat accagaacgg aatcgccgcc agcgatccca tcgtgccagg
      721 aacgattggg cttaatccac ttccgaatcc gacagcaagc agatgccacg gattactcat
      781 cttcaggcga cttttcgcga catctttatg gcgtggcaaa atggtcatat cctttccagt
      841 ctaacgtgac aggttcgccg tcacgaataa aacaaagccc ttcgatatcg gcggtcattt
      901 gcccgataca ggtaaacggt acgcccagat gaccgagagc cacatccagc gcgccacggt
      961 tcagttccgg caccgtgaaa cacaactcgt aatcttcacc gccagagagc gcccagcgca
     1021 gcgcctgttc cggtacaaca tggcgagaaa gcgcatcaga aaacggcagc aatgccaggt
     1081 caatacgtgc gccgcagtcg ctggctttca cgatatgccc gagatcggaa atcagaccgt
     1141 cagagagatc gatagctgaa tttgccagat cgcgcagtgc ctgcccctgt aaaatacgcg
     1201 gcgatggacg gagatgacgt ttgatcaagt agtccgcatc tttagcatcg gcaacctgca
     1261 aacggttttg caaaatcgcc agcccggcgg cgctatcgcc cggtgtaccg gtcacataga
     1321 tccagtcacc cggtttcgcc ccagagcgcg ttaaggctcg tcccatcgga acaaagccgt
     1381 ggatacccaa cgtcattgat aatggcccac gcgtggtatc gccgccaatg agttgcatat
     1441 cgtaataatt gagaagatca aacaaactgt cgctgaagga ctcaagccac gcttcgtcta
     1501 cgtccggtaa ggttaatgcc agcgtcagcc aggccggatc ggcccccatc gctgccagat
     1561 cgcttaggtt caccgccagt gctttataag ccagatcagc aggatcgata tcagggagga
     1621 aatggttgcc cgccaccagc gtatcagtgc tgatcgccag ggtctgtttc tcggggatat
     1681 tgagaagagc gcaatcgtcg ccgatgccca gttcgacatc aagacgagaa cttcttacac
     1741 ggtcaaaata acgggcaatc agggagaact cgccacatgc catacgttat gcctcagcag
     1801 gtaaaaaaga aaaggccggc gatcgcggaa cagtcttccg tgaatctacc ggcctggata
     1861 ttactttttg ttagggcgaa tcacaggtgc tgctttatcg agtacgccgt tgacgaactt
     1921 atggctgtct tctgcgccga acgatttcgc cagttcgatc gcttcgttaa tggccacttt
     1981 gtatggcaca tcgctacgtt tagacagttc gtacagcgca atgcgcagta ctgctttttc
     2041 tacctgtccc agttcttcca gcaggcggga caggtatggc ttcatcagtc cgtcgaggta
     2101 tgcggtatta gtcgccaccc cggccagcag ctcacggaag tacaggacgt caacgtcttt
     2161 tacatcctgt tcagccagga actggtattc aacatcagcg atgtcgttct gggacaactg
     2221 ccaggagtag agcgcctgga cggcacactc acgagcgcgg cgacgagcag caggtttcac
     2281 ggatttcccc ttactaattt caggccttga tggctttcaa tacattaatc atttcaagcg
     2341 cggtcagtgc agcttctgca cctttgttgc cagctttggt gccagcacgt tcgatcgctt
     2401 gttcaatgct ttcagtggtc agaaccccaa aagcaaccgg aatttcgctg tcctgggcaa
     2461 catgcgccag gccgttgctt gcaccaccag cgacatattc aaaatgggca gtgccaccac
     2521 gaataaccgt acccagcgca atcaccgcgt cgtatttacc ggttttagcc agtgcacccg
     2581 ccgccagcgg cagctcatag gcaccaggca cccaaacaac ggtaatgttt tcatctttta
     2641 cctgaccgat acgtttcagt gcatcaattg caccttccag caggctgtca ttgataaagt
     2701 tgttgaaacg cgcgatggtg atggcgacgc gagcgtccgg ggtagcaacg ttagcttcaa
     2761 taatgttcat tttcttcctt cgggttcgag tatggccccg caggggggcg gattttagca
     2821 taatatttcg tgcgctgctt ccctttcgag ccgggagatc atgcacccac taaatgcagg
     2881 caaacatccg ggcctacatg acgtatctct ttgaatttaa attggggggc gtcagctaat
     2941 ttctcaagcc ctggcagtgt gcataatcca cgggcgtcgc tgcctaatag tttaggtgcg
     3001 atatagacaa tcagctcatc gactaacccc gcctgcagca atgcgccagc gagcgttggc
     3061 cccgcttcca cccagatgct gttaatctgc tgtttaccca gttgcatcat cagtacaacc
     3121 agatccagat gacctttatg ctctggaatc agcaaggtac gcaccgtttc cggccactca
     3181 cgagaatctt cctgcgtacg cgcgaaccag gtttcgccgg gctgctgcac aatgtgatgt
     3241 tccggcgtca cgcgattttg gctatcaatc acaatacgta ccggctggcg gagatgatgt
     3301 tgcgggtaga gcgcctgagt ttgtacatcc agttcagacc aacgcaccgt taaggcagga
     3361 tcatccgcca gcaccgtagc gctgctggtt aaaatggcat gactttgtgc gcgcagtcgt
     3421 tgtacgtcgc gccgcgcctg aggcgaagtg atccactggc tttcgccgct cgccatcgcc
     3481 gtgcggccat caagcgatgc gccaagtttt aactgaatat aaggaaagcc ggtgcgcatc
     3541 cgcttgagaa agcctttatt caattgctcg gcttcactca tcatcaggcc gtggctgacg
     3601 tcaatgccag cctgttgcag acggtaaagt ccacgcccag cgacctgcgg gttcgggtcc
     3661 tgcatcgcag cgaccacgcg cgctacgcca gcggcgatga gtgcgtcaca gcacggtggc
     3721 gtacgaccat gatggctaca gggttcgagt gtgacatagg cggtcgcacc tttggctttt
     3781 tcacccgcca tacgcaacgc gtgtacttcg gcatgtggtt cacccgctcg ttggtggtaa
     3841 ccttcaccga caatttcgcc atctttgaca atgacgcacc cgacattcgg gttggggtgg
     3901 gtggtaaaac gtcctcgttg cgccagcttt agcgcccgcg ccatgtaata ctcgtcctgc
     3961 acggcttagt cctccaggcg cgcgatctct tcgccaaatt ctttgatatc ttcgaaactg
     4021 cggtagacag aggcaaaacg gatataggcg actttatcga gctttttcaa ttgctccatc
     4081 accagattgc caatcatctt gctcggcact tcgcgctcac cggtggcgcg cagctgcgat
     4141 ttaatatgat tgattgccat ttcgacgtca tcggaactca ccggacgttt ttccagcgcc
     4201 cgcagcattc cgctacgtaa tttttcttca ttaaacggtt cacgcacgtc gttgcttttt
     4261 acaacacgcg gcataaccag ctccgccact tcaaaggtgg tgaaacgttc attacacacc
     4321 agacactgcc gacggcggcg tacggatgaa ccctcgccca cgagacgaga gtcaattacc
     4381 ttagtgtcca cggcgaaaca gaatgggcaa tgcatacggc gtcctgacca ggtggttaac
     4441 ggaaatctat tttaccctga actgacgata caacaaaggc gcaacgcttt tgagataaag
     4501 gatcgatgct ttcgctgctt ttaccctcta ccattaggct aattgcgacg agaaagggac
     4561 gacctatgaa tattaacgtt tttcgactga tcctgctggg aagcctattc agccttagcg
     4621 cctgtgtgca gcaaagtgaa gtgcgacaga tgaaacacag cgtcagcacg ctgaaccagg
     4681 agatgacgca actcaatcaa gaaacagtca aaatcactca gcaaaacagg ctgaatgcaa
     4741 aatccagcag tggggtttac cttctgcctg gagcgaaaac accggcaaga ctggaaagcc
     4801 agatcggtac tttacgtatg tcgctggtga atattacgcc tgatacagat ggcaccaccc
     4861 tgacactacg tattcagggt gagtcgaatg atcccttgcc cgcattcagc ggaactgttg
     4921 aatatgggca aattcaggga acaatagaca actttcagga aatcaatgta cagaatcaat
     4981 tgattaatgc ccctgccagc gtcctcgccc ccagcgatgt tgatattccg ttacagttaa
     5041 aggggatttc tgtggatcag ttaggctttg tacgtatcca cgacattcaa cctgttatgc
     5101 agtaaactta tagcggggcg attttgtgcg tcccgcaaca tccttcccca tcattttgtt
     5161 actccgctta cataacccag attgatagta aaagtttgca acaagggcga aagtcagtac
     5221 aatccccgcc cgaatgtgtg taaacgtgaa cgcaatcgat tacgtaaatg atagaactgt
     5281 gaaacgaaac atatttttgt gagcaatgat ttttataata ggctcctctg tatacgaaat
     5341 atttagaaac gcaatttgcg cctttttcac tcccgcaagg gattttcaaa cagtggcata
     5401 catatgaaaa aaacattact ggcagccggt gcggtactgg cgctctcttc gtcttttact
     5461 gtcaacgcag ctgaaaacga caaaccgcag tatctttccg actggtggca ccagagcgtt
     5521 aacgttgtcg gaagctatca cacccgtttc ggaccgcaga tccgcaacga tacctacctt
     5581 gagtacgaag cattcgctaa aaaagactgg ttcgacttct atggttatgc ggatgcgccg
     5641 gtattcttcg gcggtaactc cgatgcaaaa ggtatctgga accacggttc tcctctgttt
     5701 atggaaatcg aaccacgttt ctccatcgac aagctgacca atactgacct tagcttcggt
     5761 ccgttcaaag agtggtactt cgcgaacaac tacatttacg acatgggtcg taataaagat
     5821 ggtcgccaga gcacctggta catgggtctg ggtaccgata tcgacactgg cctgccgatg
     5881 agcctgtcca tgaacgtcta tgcgaaatac cagtggcaga actatggcgc agcgaacgaa
     5941 aacgagtggg acggttaccg tttcaaaatt aaatactttg tgccgattac cgatctgtgg
     6001 ggcggtcagc tgagctacat cggcttcacc aacttcgact ggggttccga tttaggggat
     6061 gacagcggta acgcaatcaa cggtattaag acccgtacta ataactctat cgcttccagc
     6121 catattctgg ctctgaacta cgatcactgg cactactctg tcgtagctcg ttactggcac
     6181 gacggtggtc agtggaacga cgatgcagaa ctgaacttcg gcaacggcaa cttcaacgtt
     6241 cgctctaccg gctggggtgg ttacctggta gtaggttaca acttctgatt atgaaaatgc
     6301 cgggatttat tcccggcatt tcagattgtt aacaacgtac gctttgttca tgccggatgc
     6361 ggcgtaaacg ctttgtccgg cccacaaaag cacgtaaatt caatatattg ctgaaataac
     6421 gtaggcctga taagcgtagc gcatcaggca attttacgtt ttaatcactt cttcttgttc
     6481 atcatcgctt tcaggtcagc gaatgggttg tacttcgctt caccgacatc tttctgcgca
     6541 tcttcccctg cgataacggt cgtaccatac tgatccgctt cggtatattt cgaatgctca
     6601 tgatcgtggc aatagagaca aagcaattcc cagttactgc catcttccgg gttattggta
     6661 tggtcgtgat caatgtggtg aacggtaagt tcacgcaggt tggaataaac aaactcgcgg
     6721 gaacagcgac cgcagaccca cggatagatt tttaatgctt tttcgcgata gccgctttct
     6781 aaccgcgcat agttttttgg gatgatagcc atgaaaagtt acctggatga aatgtagggt
     6841 tacgcgcagt ttcccaagat gcggggattt tcgcaatcct gagtcaagaa attctcacat
     6901 taccagtgac ggcgaggttc catcaactga tgaagaatgc gcgcaatgag aatgtcatcg
     6961 actcttataa cgtaataaac agtgtgaaac ctaaatgcga tcttctttac ctctggaatc
     7021 gcatcatagt cttgccctat ctcgggcatt tgagagagtg tatccagaaa agtgccgaga
     7081 gagtcggtat agcgatccgc ctgctcatca ccaaactttc ccagcgtgta gtcatagatc
     7141 cctctgaaat cttcaacagc ttttccgcta agcttataca cgatgttttt gcttcctttc
     7201 tgctgcgata tcacgaagcg tcatcgaact ttcaccgctc tcaagacctt caataacagc
     7261 atttctgaca gcttcatccc tggattcttg ttgttccaat agacgaagtg ccgaacgcat
     7321 tacttcactg gccgagccat aacgaccaga ctggaccatc cgggtaataa aactatctaa
     7381 ttgttcgcca acgcttactg aggtaatttt gcgcataagc gatctccacg tattaactat
     7441 taatacaaag tatatgtcag aacgctttct acaaccaaac cgtattcagt tgcctgtgaa
     7501 acaggcaaaa aaaaaccgca gcgcaaatac gccacggttt ttttaacaga acggatttaa
     7561 aacaccatta cggcagaatt gacggctgat ccgccccttc tttttccact ttctgctgca
     7621 acatgtgttc gcgcttcata cccagtttca gagccaacgc agatgccaca tagatggaag
     7681 atgcagtacc gatggaaaca ccgataagca tggtcagcga gaagccttcc agtaccggac
     7741 caccgaagag atacagcatc aggataacca tcaaggtagt accggatgtg atcaaggtac
     7801 ggtgcagcgt ctgggtcaag gacacgttaa agatttcgta aggcgtaccg cgacggatct
     7861 tgcggaagtt ttcacgaatt cggtccgata ccacgatact gtcgttaagc gagtaaccga
     7921 taaccgacat caacgatgcc acaatggtca ggtcaatctc gatatggaat aacgacaaaa
     7981 tacccagcgt aatgataacg tcgtgcgcca gcgcaataac cacccctgcc gccagtcgcc
     8041 actcaaagcg gaaacctacg tacacgagga tagacagcag cgctgccatc aacgccatcg
     8101 caccggtttg cgcaaggtct gcccccacgc tcggaccgac gaactcaata cgcttcactg
     8161 ctgcattctg attggtggat tcgttaatca ccttcagaac ctggctgccc agcacctgac
     8221 cgccggtttc gccttcagca ggcggcatac ggaccatgat gtcgtggctg ctaccaaagt
     8281 tttgcagcat cggctcttca aaaccggctt tttgcaatgc atcacgcatt acgtcaattt
     8341 cagccggttt ttcgagcgta atttcgataa ccgtaccacc ggtgaaatcc agcccccagt
     8401 taaagccgcg cacgcccata ataacgatag cagcgattaa cagcagacca gagatgccaa
     8461 aagcccagta gtcccagcgc ataaagtcat agactttacg gccgtggttt agttgttcaa
     8521 cagtatattc ctgtgccaca tcgcactcct cagattgaca gcttcttgac gcgcttgccg
     8581 ccatatagca ggtttacgat ggcacgcgta ccgacaatcg cggtaaacat cgacgtcgcc
     8641 acaccgatac cggtagtaat cgcgaaccct ttaattgccc cggtacccac tgcgtacagg
     8701 atgatgactt taatcagcgt ggtgatgttc gcatcgaaga tagaactgaa tgcgccacga
     8761 taaccttcat caattgcctg ttgaacagta cgtccgttgc tcaactcttc tttaatacgt
     8821 tcgttgatca gtacgttcgc atcgaccgct accgcaaggg ttaagacgat acccgcgata
     8881 cctggcatac tcagtgttgc gcctggcaac agcgacataa taccgacgat caagatcaag
     8941 ttggcaatca gagcactggt cgcaatcaga ccaaacttct tatagaagat gatcatgaac
     9001 agaatagaaa ccagcagacc ggcaaggcaa gcctccagcc cctgctcaat gttctgcata
     9061 cccagggtcg ggccaattgt acgttcttca acaatctgaa tcggcgcgat caacgcaccg
     9121 gcacgcagca gcagtgacag ctgacgggct tcgttcgggt tgttgatgcc ggtgatacgg
     9181 aagctgttac ccagacgaga ctggatgttg gcgatgttaa tcacctcttc ctgtttcacc
     9241 agaaccgcac gaccatttgc atctttctta ccgctgtctt tgtactccac aaacagggtt
     9301 gccatcggtt tgccgatgtt gtccttggtg aagttagaca taatgttgcc accagcgcta
     9361 tcgagcgaga tgttaacctg cggctggttg tattcgtcct ggctggaagt ggagtcggtg
     9421 atatggtcac cggtaagaat tacgcgtttg tacagcacaa ccggctgacc ttcgcgggtc
     9481 tgtttcactt cagagtcgcc cggtacgcga ccggatgccg cagcggcctg atcaacgttg
     9541 gtgtttacca gacggaattc cagcgttgcc gtcgcaccca gaatctcttt cgcacgcgca
     9601 gtgtcctgaa tacctggcag ttcaacaacg atacggtcag caccctgacg ctgaaccacc
     9661 ggctccgcca cgccaagttg gtttacacgg ttacgcagga tattgatgtt ctgctgcacc
     9721 gcatattcac gcgcttcact cagacgagca tcgctcatta ccgcacgcag ctggttgctg
     9781 ccctgactgc taatcaccag gtccggatga cgcttgctca gatacgcaat ggcttcatca
     9841 cgagctttag catcgcggaa agtgatgctc aggccgtagt tattttcttt acgaacggtg
     9901 gtatacggga tgcctttttc gcgcaggtcg ctgcgcaggc tatcgatatt ttgttcctgg
     9961 agtttgccaa gcgcggtgtc catatccact tccatcagga agtgaacgcc gccacgcagg
    10021 tcaaggccga gcttcatcgg ctcagcgtga atagctgcca gccagcgcgg cgttgccggg
    10081 gcaaggttaa gcgccacgac gtatttgtca cccataacgc ccattaatgc ttcacgagcg
    10141 cgcagctggg tgtcagtgga gtcgaagcgc gcaagaatag cgccctcttc cagtgccaca
    10201 gacttagcag ttattttttc ttcttgtaac gttttctgga cctggatcag cgtttgctca
    10261 ctggcggcga ctccgcgcgc accagtgatc tgaacagccg gatcctcacc aaacaggttg
    10321 ggaagcgcat acagcagacc gatgacaatc accacgatca gcatgacgta cttccacaaa
    10381 ggataacggt ttaacacggc aattccctta gggaaaaatt ttaattacag cgccttcatg
    10441 gtgcctttcg gcaggacggc agctacgaag tcacgtttaa taactacttc agtggtgtca
    10501 ttcagcgcga tagcaatgta gccgttttcc gctactttgg ttacgcgacc aaccaggcca
    10561 ccgttcgtca gaacttcatc acctttggca atggagtcca tcagcttttt gtgttctttg
    10621 gtgcgcttct gctgtggacg caggatcatg aaatagaaaa tcagaccgaa taccaccagc
    10681 atcaaaatca aagacatcgg gctaccttgc gccggtgcac ccgttgccgc taccgcatca
    10741 gaaataaaaa agctcattaa atttccctca ttattaatat taatcaacgt tcaaaggtgg
    10801 tacttctcgc ccctgacgct ggtaaaaatc agttacgaag ctctctaatt taccctcttc
    10861 aatagccttg cgtaaacccg ccatcaaacg ctggtagtaa cgaaggttat gaatggtatt
    10921 gagtcgcgcg cctaatattt cgttgcaacg gtcaagatga tgcaagtaag cgcgtgaata
    10981 attgcgacag gtgtagcaat cacactcagg atcgagtggg ccagtatcgc tcttatactt
    11041 cgcattgcgg attttcacca cgccatcggt cacgaacaaa tgaccatttc gggcgttgcg
    11101 ggttggcatt acgcagtcaa acatatcgat accacgacgt acgccttcaa ccaggtcttc
    11161 tggtttacca acgcccatca ggtaacgcgg tttgtctgcc ggaatttgcg ggcatacatg
    11221 ctccagaatg cggtgcatat ctgctttcgg ctcacccaca gccagaccgc cgacagcgta
    11281 gccatcaaaa ccgatatcta ccagaccttt aacagaaata tcacgtaaat cttcgtaaac
    11341 gctgccctga atgataccaa acagcgcatt tttgtttccg agactgtcaa aacgctcacg
    11401 gctacgcttc gcccaacgca gagacatctc catggagcgt tttgcgtaat cccagtcagc
    11461 aggatacggc gtacactcat caaagatcat gacgatatcc gaaccaagat cgtactgaat
    11521 ctccatcgac ttttccgggt cgaggaaaat cgggtcgccg ttgatcgggt tacggaagtg
    11581 cacgccctgt tcggtgattt tacgaatatc gccaaggctg aagacttgga agccgccgga
    11641 gtcggtgagg atcggtccct tccactgcat aaaatcgtgc agatcgccgt gcagtttcat
    11701 gatttcctgg cccgggcgca gccacaggtg gaaggtgttg ccgaggataa tttgcgcgcc
    11761 agtggcttca acttcttccg gcgtcatccc ttttacggtg ccgtaggtgc caacaggcat
    11821 aaaacaaggc gtttccacta cgccacgatc aaagaccagg cggccacggc gtgcgcgacc
    11881 gtcggtggtg tccagttcaa atttcatttt ttctccacta cgtcagaaaa acagtccaac
    11941 gtttaaacca gcgccgcgga attactcccc gacgcgctca ttaattgcct gcggattgta
    12001 cgtgataaac atcgcatcac cgtaactaaa aaagcgatat ttctcttcta ccgccgcttt
    12061 ataggcattc attgtgtgtt gataaccggc aaaggccgaa accagcataa tcagcgtcga
    12121 ttccggaagg tggaagtttg tcaccagcgc atcgaccact ttgtactgga agcccggata
    12181 gataaagatt tgggtatcgt caaagaacgg ttcgatgaga tcgtttttcg ccgcctgagc
    12241 cgcgctttcc agcgaacgta ctgaggtggt gccaaccgca atcacccggt taccgcgcgc
    12301 tttcgccgcc agtaccgcgt ctaccacatc ctgcggtact tcagcgtatt ccgagtgcat
    12361 gatgtgatct tcaatggtgt cgacgcgtac cggctggaag gtgcccgcac caacgtgcag
    12421 ggtgacgaac gccatctcca cgcctttggc gcgtaatttt tccagcaaag gttcgtcaaa
    12481 atgcagacca gcggtcgggg ctgcaaccgc gcccggtttt tcgctataaa cggtttgata
    12541 aagttcgcgg tcagcgtctt cgtccggacg gtcgatatac ggcggcagcg gcatatggcc
    12601 gatgctgttg agaatatcca gcaccgagcg ttcatcatta aattcgactt caaacagtgc
    12661 gccgtggcgc gcggtcattg ttgcgttaat actttcgtca tcgcccagca gcagttctgc
    12721 gccaggtttt ggcgctttcg aggcgcgaat atgcgcaaga atgcgtttgt cgtcgagcat
    12781 ccgttcaacc agcacttcaa tcttgccgcc gctggcttta cgcccaaaca ggcgcgccgg
    12841 gatcacccgg gtattattaa aaaccagaag atcgccgggg ttgagcttat cgagtaaatc
    12901 ggtgaaagta ccgtgcgtca gcgcgcccgt cggcccgtcc agcgacagta aacgacagct
    12961 actgcgttca ggcatcggat agtgggcaat cagggattcg ggcaattcaa aggagaaatc
    13021 ggtaacgcgc atgacactga ctcagactaa aataagaggc gggtagtcta gtgccggggc
    13081 gctctccctg caacctttac ccccctttgg atagaatata atcgatgaat tttttagctc
    13141 acctgcattt agcccatctc gcggaaagct cgctttccgg caatttgctg gctgatttcg
    13201 tacgcggaaa tcctgaagaa agttttccgc ccgacgtcgt ggctggcatt catatgcatc
    13261 gacgtatcga cgtattgact gacaatctgc cggaagtccg cgaagcacgg gagtggtttc
    13321 gtagtgaaac tcgccgcgtt gcgcctatta cgctggatgt catgtgggat cactttcttt
    13381 cccgtcactg gtcgcagctg tcgccggact ttccgctaca ggaatttgtt tgttatgccc
    13441 gcgagcaagt gatgacaatt ttgccggact caccgccacg ctttatcaac ctgaacaact
    13501 acttatggtc agagcagtgg ctggtgcgct atcgcgatat ggatttcatc cagaacgtgt
    13561 taaacggcat ggcaagccgt cgcccacgtc tggatgccct gcgtgactcc tggtacgatt
    13621 tagacgccca ttatgccgcc ctcgaaaccc gcttctggca gttttatcca cggatgatgg
    13681 cgcaggcgtc acgcaaggcg ctctgaaact tcaaactccg gcgtcactat tttttagcat
    13741 tcgcatccag cggggcggca taacaacggt aagaaaaaca ttcgctggaa tgattggcga
    13801 tatctttaaa gttgctttca gcaaccccct cgaacatgag taccgttctg gacaactctc
    13861 cgttttcatt tatatgtgga aaatcaaatt ggctactgaa tcgccataca tctgtctttt
    13921 ctggctcgct cttcacagag atatgtcgat ggaggacccc tcctgtaaac gtgcgccaga
    13981 acaattgttg atcaaaatct tgtaatatcg cagacttgga tgctatttga cgtagtaaac
    14041 ctgcataccg tggactgcca ttacgagcaa cattatgcaa accctgacta aaagcatcaa
    14101 acaaacaata gatgtgatca ctagtgaaaa aaggtcttaa ttttttttgt tcttcatcgc
    14161 tgtaatccag gagcatctca ttgatttctt ccggtgagaa ttttaagaca aaattatcct
    14221 cagaatttgt ttgtttcttg tttttttcaa tattcttctc ctcaatgctc ttcgcatttt
    14281 gtttcgcaat tatttccgca gtttttttcg caatttcttc cgcaattttt tcccgagtta
    14341 ttgattttag actaacacca tcgatgtcaa ccggtgttgt tttcagatct ctctgcgcgt
    14401 cttccaaatt tgtcttatag agtttgtgca gtttatcggc agatacgtat ccggatgaag
    14461 aaatctggtg tgtacgcatg gggggctgtt cacaaagcat cgaggctata tcgtctttaa
    14521 actcttttac attaaagttc tcggctttaa ccttccttgt tataaacccc gcatcggcaa
    14581 ttttcttctc taattgtttt gtacgtttag aataataagc gttgttatca taaaactttt
    14641 caagaagggc ccactcctca agttgcctta tgtgattttt caactgttga tgatctattt
    14701 tatattcatg tgtactagaa tcattttggt tgcaggagtt acagattttt aagcataatt
    14761 ttccatcaga tttatcatct atggtatcag tgaaaatata ggacacttca atactctgcc
    14821 cgttcttatc agtcatctca tatgaccatt gtcccgttcg tttcgcattc ttaatgactt
    14881 cgatcgactc tacaggacaa ccatgaagaa tgggcagaat tttatcagga tgcttaatga
    14941 gtaaaattat tatatttaca atgtttgaaa agaaattttt gctatttttt atttcatcga
    15001 ttttttccgc tgaaatgttt actaagtttt catttccgga aatgaaaagc gatttgccat
    15061 aggtgctttt tttaaattgt tcttctgttt tagaaaagac atcctcttta aaaggtggca
    15121 actcagtacc agtaataaac ggtttcaata aattcgaatt tgaaattgtc atatctttgc
    15181 tccactacag ggttatcctg gagcatgttg tacataacat catgggcaaa caataccaaa
    15241 aatcgctcat atcgatcggg acaaatattt aacaaattca gccctgacat tgagcagctt
    15301 ttttgcgctg cgtagtgtca gggctggtga ctaacgacgt aacaaataaa acagatgctt
    15361 tagttcatcc acaccgtagc cgaaatggca ggcaaagcca gaatcccgtc tgtcagttgc
    15421 ccatggcctt ctttgcgttg ccattgcacg acattaagaa acggtgacgc gggtagcacc
    15481 acttcacagg cttcgccacg gttaattgcc accagtacac gctgctgatt cagcacgcgg
    15541 acaaatacca ccacgttatc ttccgcatac agcacctgac agccgccacg acgcagcgcc
    15601 tgacttttct tacgcagcgc aatcattcgc tggtacaacg cgaataacgc catatcctgt
    15661 ttttccacct gccaggggaa cggcttacgg caaaacggat catttttgcc atccagtccc
    15721 acttcatcac cgtaataaat gcacggtaca ccaggccagg tgaacagcca gaccactgcc
    15781 aggggcaggc gcgcaacctc ccgaccgagc agcgttttaa atcgcgcagt atcgtggctg
    15841 tcgagctgat taaacatacg taattgctgt tgatgagaaa gccctgcgcg gtaattatcc
    15901 atccaggcca tacaggtttg agcgtcaatt tgctgcggat cgtaagagat gtcggtattg
    15961 gcaagaaatc cccacaacgg gaatgtgaag ccgcgatagt tcatggcggc atcttccaca
    16021 tcggcctgta accattgccg tgcatcgcca aaatgctctc caactatata cgcttccggc
    16081 tgggtttctt tcgccgcttc ggtgatccca gcaacgtgct gcaaattatt gcgcgcccca
    16141 cccgcttccc ccagcatatg caccacatcc agccgccagc cgtccatatt ccacggcgct
    16201 ttcagccagt ggcggacaat actgtcttcc ccgcgataaa tttcattcac cagactttcc
    16261 gactgataat ccagcttcgg caagctggca tagccaagcc agtcgagcgc cgtgccatca
    16321 tcactaaacg agtaccagtc gcgccagggc gattcggggt tgtgacaagc tccccccgtg
    16381 ccacgattgt gcctgtcaaa ccaggcatgg gaatcgccac tgtggttaaa cacgccatcc
    16441 agcaccagcc gcattcccag ctgctgcgta ttgtggcgta agcgcagcaa cgccccatca
    16501 ccgccaaact gcggatcgac atggcgataa tcctcggtat cgtatttatg tacgctggga
    16561 gctttaaaca ccggattgag atacagcgcc gtcacgccaa gctttttcag atacggcagt
    16621 ttttcgctta tcccgtccag atcgccgcca tagaacgttg atccgcccgc ctgcgccgtg
    16681 accggttcat cccagtcacg caagatgatc tcttgtccgg ctgcatgatg gtaatagaca
    16741 tgatcctgtt cagcttcacg aggaagacta cgcgcaaaac gatcagggaa gatctgataa
    16801 aaaatctgat ccgcagccca ttgtgggccg atatccggta catcgacggc aaactgctcc
    16861 agtcgtgccg gcggcattcg gctgaagccc tgcggtgtaa accaacgctg gcgatcgtgc
    16921 cacagcagtt tgaaactgta acgccgccgg ggttgtccgc tggagagatc aatcgccgca
    16981 cgccatgcgg tgacgcctgg ctgcggctga ctgcgctgct tatgcattgg tactgacatt
    17041 tcttcgttat cgtgttctgt acgcagcata atgcgctgcg gtgggtcttc gcccgtcagc
    17101 cacagtgtaa tgagcagttg atctttgctt tgtttaacaa atgggggcac cggcaggtgc
    17161 catgcattta acatcataat tcccctgtga tgaaaatctg atcaccttgc catagcctaa
    17221 tgcaggatca ctcatcatct gaaggatatt tgggggagga ggacgggggc ggataacttt
    17281 cggaatagcc ggatcaggat ttcctcctga accggcttac agaacgcaag tattattggt
    17341 tttcagccag ctgacgatcg tggcgacgct taaacatcca gccaatcaac agcacaacaa
    17401 tccacgcgaa accgacatac agcgagatac gcgtatccgg gtgataacca atcaacccga
    17461 taataaagag caggaaaatc agcccgccga tggtcgttgc taccccaccc ggcactttaa
    17521 atttcagcgc cttaacttct tctggcggca aacggcgacg gaaggcaatt tgcgacagca
    17581 ggatcataat ccacacccac accgtggcga aggttgccag cgaagcgatc accaggaaga
    17641 cgttttccgg catgatgtag ttcagataca ccgcaaacag cagcgcggta gtcatcacca
    17701 gcaccgtaac ccacggaata ccgcgacgcg acgttttgct gaaaattttc ggcgcgctgc
    17761 cctgctctgc cataccgtgg agcatacggc ctacgccaaa tacatcactg ttaattgccg
    17821 acagcgaagc agtcagcaca acaaagttaa gaatgctggc ggcaaaggta atgcccatat
    17881 gctggaacgt cagcacgaac gggctaccgg cagtgccaac ctgattccac gggtagatag
    17941 acataatgac gaacagcgta ccgacgtaga acaccagaat acgcatcggc acggagttaa
    18001 tcgcacgcgg tatcgatttc tcaggatctt tcgcttcacc ggcggtaatc ccgataattt
    18061 cgatcccacc gtaagcaaac atcaccattt gcaacgacat taccatgcca agccagccgt
    18121 tactgaagaa gccgccgttg ctccacaggt tatgaatacc ggtcggttgc ccgccgttgc
    18181 caatccccca gatgatgatg ccgaaaccgg cgacaatcat gatgatgatg gtggcgactt
    18241 taaagaacga gaaccagaat tccagctcac cgaatacctt cacgctcatc aggtttacgg
    18301 cgcaaatgat cagcaccacg ctcagtaccc aaatccagtg cggcaccgtc gggaaccaga
    18361 cacccatata gataccaaaa gcggtcacat cggcgatggc gacaataagg atttcaaagc
    18421 agtaggtcca gccggtaatg taacctgcca gcgggccgag gttttcctgc gcataacgcg
    18481 agaaagagct ggcggccggg ttatgtaccg acatttcccc cagcgcacgc ataatgatat
    18541 acgccgcgat accaccgata atataggcca acaacacgct cggaccggcc attttgatgg
    18601 cgtctgccga accgtaaaac agcccggtgc caattgctga acccagtgcc ataaagcgta
    18661 tgtggcgggt acttagccca cgctttagct tgttcttact ttccatcaat tcctaaccca
    18721 tcaacacaat aaaaaaccac ggggcatcgc agccccgtgg ttaaaacaaa tgttcagtga
    18781 tttagtgagc gctggaggtc acctgacgac ctgccgcgcg atcccagata atggccagaa
    18841 ccaccatcac cactgttggc attaaccacg ccagaccttg ttcggccagc ggtaaacgct
    18901 gcgcccagga cggtaagata tcgctgaatg cagatgcttt gatcccgtcg agaataccaa
    18961 aaagcaggct gataaacatc ggcggagcaa tcacgcggga cgaattatgc caccatgagc
    19021 gtgtaaaact taatacaacc agtgcgatac acggcggata aatagcggtc agcaccggta
    19081 cggagatctg gatcagctgg cttaagccga ggttagaaac caccatcgag aagccgccga
    19141 ggataaacac cagcgtacga taagagagcg gtacgtactg ggcaaagaat tctgcacaag
    19201 cacaggtcag gccaactgcc gttaccaggc aggcgatgaa gattaacgcc gccagcagga
    19261 agctaccgcc gccgccaaag gtgtgctgaa cgtaagcatg cagaatagca gcgccgtttg
    19321 cagactgatc gaccagcgac gcgctgtctg aacccagacg gaacagcgcc aggtacagca
    19381 gagtcagacc aacacccgcc atcaggccag cccagacggt ataacgggtc agtaggcgcg
    19441 cttcggtaac gccacgagaa cgcgccgcgt taacaataac gataccaaac accattgcgc
    19501 ccagcgtatc catggtcaga tagccgttga cgaagccgtt agaaaacgca gcgttttgat
    19561 aagcctcagt cgccgtgctg atagaacccg ccggccagac aatcgcggca acagacagga
    19621 tgaccagcgc gataattttc agcggcgcaa ggaagttgcc cacggtatcc agcagcttgc
    19681 ccggatagag cgaaaccaga ataacgatag cgaaatagac caggctgtag ataaacagcg
    19741 gcagcgcgga atcacccgtc agcggcgcaa tccccacttc aaaagaaacg gtagctgtac
    19801 gcggcgtagc gaaaagcggc cccaccgcca ggtaacaaac cgttgccagc agtacgccag
    19861 cgactttacc gattggcgtg ctgaggctgt caacaccgcc gccaactttt gccagcgcca
    19921 ctaccgttaa taccggcagg ccaacggcag taatgaggaa gccgaatgcc gcagtccaga
    19981 cgtgttcgcc tgcctgtaag ccgaccattg gagggaaaat aatgttacct gcgccgacga
    20041 acaacgcaaa tgtcataaag cccagagcga tgatatcgcg cgatcttaat tgatgggtca
    20101 taaatcatac tgcctgtgga tgtggtgttg taacgttaaa tttttgtgcc aatccctggg
    20161 gatgatacag cggtatattt aaacaatttt agcgggaaat actcccaacc aggcgcggcg
    20221 aagaatagtt tttcgattta ccgcgttaat gcagtcagta tgacagcacc tgaagcgcaa
    20281 tttaaacgct tataacgttt taaagcaagg agggatctaa aaaccagagg tatatagcga
    20341 taagaaatca ccaaccagta taaaacacta tttccgtgaa aaagacatgg ctaatcatgc
    20401 gaacaaaaaa tcaccaaccg ttgtaaaatc taacgcccgt attttgactg caaagaaaat
    20461 aagccagcat cggctggctt atggcaataa aagatgacaa aggcggatta atcgctgttt
    20521 ttgacaatta aacgttccgg gataacaaaa ctgaaacgtg ttccttttcc tactgtactc
    20581 tcaatattca ggcgactttc gtgatgattc accgcatgtt tcacgatcgc taaccctaat
    20641 ccgctaccgc cggtttgccg ggaacgcgct ttatcaacgc gataaaaacg ctcggtcagg
    20701 cgcggaatat gctccggtgc aatgcccggt ccgttatctt caacgctaaa ttcggcaccg
    20761 tgcggcactc gctgccagcg tacggtgata tgcgtgcctt ccggcgtatg attcacggcg
    20821 ttatagacca ggttcgaaat cacactgcgt agctgatctt cgttgccaga caccttgagg
    20881 ccgttatcta tctcgaaggt aaatgtctgt tttttctgac tcagagtctg agcctcgcgc
    20941 tcaacaacgc gcagcatcat cggcacatca accttttcat tgagcaattg cgtcggtgcg
    21001 gcttcaattt tcgacagcgt tagcaactgc ttcaccagcc cttccatccg ctgggtctgc
    21061 tcgcgcatgg tgtgcaacgc tttttcgcgt accgcgcctt ccagcggctg ctcatccatc
    21121 atctccaggt aaccctgtaa cacggtcaat ggcgtacgta actcatggct cacgttggca
    21181 aaaaagttac gccgcgcccc ttccagttga tgcatttgcg tgacatcacg cgccaccatc
    21241 agcaactgtt tgtgggtata aggcatgacg cgaatttcca gatgccgccc ggtgttgagc
    21301 accagattga gcgggcgaga aaaatcacgc gttttcaaat attgcgtaaa ctccgggtaa
    21361 cgcagcaggt taaggatgtt ctgcccgtta tcttccggcc agcgcaaacc aagaatctgt
    21421 tgcgccagac cgttacacca gaaaataccg ccctcttccg tggtcagcac caccgcgtcg
    21481 ggcagcgact ccgcgccgct acgaaagcgt ttaatcagat tgcccagttc acggcggcgt
    21541 tttttatttc gcagctgcat ctggtgtaag ccgtatagta gcggttccca gctaccacgc
    21601 cccggtggcg gggtcatact gcgatccacc cacagccacc atgaaaggcg caataaattc
    21661 cagaaatgcc agataagcag tcctgttacc gatgccagca aaaaccaggg caggtaacca
    21721 aaaaatgcac ccaggatgaa agccgggagg cagcaaagta gcagctccag caccagcctt
    21781 ttccacgaca gccgttccag cacgtaagat actccagtta agaaatcata agccctgctc
    21841 tgcgtccgat gagcaaggcg ttaaaagcgg gttgaaaaac gatatcctgt accgcgcacg
    21901 gtctgcacca tgcggtcatg cccgccgggc tccagtgctt tacgcaggcg acgaatgtgg
    21961 acatcgaccg tgcggtcttc cacatacacg ttagttcccc agacgtggtt taacagctgc
    22021 tcgcggctgt acacgcgctc aggatgcgtc ataaaaaagt gcagcagttt aaattctgtc
    22081 ggccccatct ccagcggctc ttcgcccgcc atcactcggt gagatgtcgg gtcgagactt
    22141 aatccctgca tctcaatcac ctcttccacc gccattggcg aaatacggcg cattaccgct
    22201 ttgattcgcg ccaccagctc cttcggcgaa aacggcttgg tgatatagtc atccgcgccg
    22261 gtttcaaggc cgcgcacgcg atcttcttct tcccctctgg cggtcaacat caccactgga
    22321 atatcccggg tcatcgactc gcgcttgagg tgtttgatga actggatacc ggagccgcca
    22381 ggtaacatcc agtcaaggag aattaaatcc ggccagggtt cattcagttg attcacagca
    22441 ctgtcataat cttccgcttc gaccggctga aagccatttt gttcgagcac gaagcagacc
    22501 atttcgcgaa ttggagcttc atcttctacg accagaatac gtctcgccat gatttgccct
    22561 gttgtaataa ataggttgcg atcattaatg cgacgtcatt atgcgtcaga tttatgacag
    22621 atttatgaaa agctcgtcgc acatatcttc aggttattga tttccgtggc gcagaaaaaa
    22681 gcaaatggca catctgtttg ggtataatcg cgcccatgct ttttcgccag ggaaccgtta
    22741 tgcgcatcct tcacacctca gactggcatc tcggccagaa cttctacagt aaaagccgcg
    22801 aagctgaaca tcaggctttt cttgactggc tgctggagac agcacaaacc catcaggtgg
    22861 atgcgattat tgttgccggt gatgttttcg ataccggctc gccgcccagt tacgcccgca
    22921 cgttatacaa ccgttttgtt gtcaatttac agcaaactgg ctgtcatctg gtggtactgg
    22981 caggaaacca tgactcggtc gccacgctga atgaatcgcg cgatatcatg gcgttcctca
    23041 atactaccgt ggtcgccagc gccggacatg cgccgcaaat cttgcctcgt cgcgacggga
    23101 cgccaggcgc agtgctgtgc cccattccgt ttttacgtcc gcgtgacatt attaccagcc
    23161 aggcggggct taacggtatt gaaaaacagc agcatttact ggcagcgatt accgattatt
    23221 accaacaaca ctatgccgat gcctgcaaac tgcgcggcga tcagcctctg cccatcatcg
    23281 ccacgggaca tttaacgacc gtgggggcca gtaaaagtga cgccgtgcgt gacatttata
    23341 ttggcacgct ggacgcgttt ccggcacaaa actttccacc agccgactac atcgcgctcg
    23401 ggcatattca ccgcgcacag attattggcg gcatggaaca tgttcgctat tgcggctccc
    23461 ccattccact gagttttgat gaatgcggta agagtaaata tgtccatctg gtgacatttt
    23521 caaacggcaa attagagagc gtggaaaacc tgaacgtacc ggtaacgcaa cccatggcag
    23581 tgctgaaagg cgatctggcg tcgattaccg cacagctgga acagtggcgc gatgtatcgc
    23641 aggagccacc tgtctggctg gatatcgaaa tcactactga tgagtatctg catgatattc
    23701 agcgcaaaat ccaggcatta accgaatcat tgcctgttga agtattgctg gtacgtcgga
    23761 gtcgtgaaca gcgcgagcgt gtgttagcca gccaacagcg tgaaaccctc agcgaactca
    23821 gcgtcgaaga ggtgttcaat cgccgtctgg cactggaaga actggatgaa tcgcagcagc
    23881 aacgtctgca gcatcttttc accacgacgt tgcataccct cgccggagaa cacgaagcat
    23941 gaaaattctc agcctgcgcc tgaaaaacct gaactcatta aaaggcgaat ggaagattga
    24001 tttcacccgc gagccgttcg ccagcaacgg gctgtttgcc attaccggcc cgaccggtgc
    24061 ggggaaaacc accctgctgg acgccatttg tctggcgctg tatcacgaaa ctccgcgtct
    24121 ctctaacgtt tcacaatcgc aaaatgatct catgacccgc gataccgctg aatgtctggc
    24181 ggaggtggag tttgaagtga aaggtgaagc gtatcgcgcg ttctggagcc agaatcgggc
    24241 gcgtaaccaa cccgacggta atttgcaggt gccacgcgta gagctggcgc gctgcgccga
    24301 cggcaaaatt ctcgccgaca aagtgaaaga taagctggaa ctgacagcga cgttaaccgg
    24361 gctggattac gggcgcttca cccgttcgat gctgctttcg caggggcaat ttgctgcctt
    24421 cctgaatgcc aaacccaaag aacgcgcgga attgctcgag gagttaaccg gcactgaaat
    24481 ctacgggcaa atctcggcga tggtttttga gcagcacaaa tcggcccgca cagagctgga
    24541 gaagctgcaa gcgcaggcca gcggcgtcgc gttgctcacg ccggaacaag tgcaatcgct
    24601 gacagcgagt ttgcaggtac ttactgacga agaaaaacag ttacttaccg cgcagcagca
    24661 agaacaacaa tcactaaact ggttaacgcg tctggacgaa ttgcagcaag aagccagccg
    24721 ccgtcagcag gccttgcaac aggcgttagc cgaagaagaa aaagcgcaac ctcaactggc
    24781 ggcgctcagt ctggcacaac cggcacgaaa tcttcgtccg cactgggaac gcatcgcaga
    24841 acacagcgcg gcgctggcgc atactcgcca gcagattgaa gaagtaaata ctcgcttaca
    24901 gaacacaatg gcgcttcgcg cgagcattcg ccaccacgcg gcgaagcagt cagcagaatt
    24961 acagcagcag caacaaagcc tgaatacctg gttacaggaa cacgaccgct tccgtcagtg
    25021 gaacaacgaa ctggcgggtt ggcgtgcgca gttctcccaa caaaccagcg atcgcgagca
    25081 tctgcggcaa tggcagcaac agttaaccca tgctgagcaa aaacttaatg cgcttgcggc
    25141 gatcacgttg atgttaaccg ccgatgaagt tgctaccgcc ctggcgcaac atgctgagca
    25201 acgcccactg cgtcagcgcc tggtcgcgct gcatgggcag attgttcccc aacaaaaacg
    25261 tctggcgcag ttaatggtca ctatccagaa tgtcactctg gaacagacgc aacgtaatgc
    25321 cgcacttaac gaaatgcgcc agcgttataa agaaaagacg cagcaacttg ccgatgtgaa
    25381 aaccatttgc gagcaggaag cgcgcatcaa aacgctggaa gctcaacgtg cacagttaca
    25441 ggcgggtcag ccttgtccac tttgtggttc caccagccat ccggcggtcg aggcgtatca
    25501 ggcgctggag cctggcgtta atcagtctcg attactggcg ctggaaaacg aagttaaaaa
    25561 gctcggcgaa gaaggtgcgg cgctgcgcgg gcaactggat gcattaacga agcagcttca
    25621 gcgcgatgaa aacgaagcgc aaagcctccg acaagatgag caagcactta ctcaacaatg
    25681 gcaagccgtc acggccagcc tcaatatcac cttgcagcca caggacgata ttcaaccgtg
    25741 gctggatgca caagatgagc acgaacgcca gctgcggtta ctcagccaac gacatgaatt
    25801 acaagggcag attgccgcgc ataatcagca aattatccag tatcaacagc aaattgaaca
    25861 acgccagcag cagctcttaa cggcattggc gggttatgca ctgacattgc cacaggaaga
    25921 tgaagaagag agctggttgg cgacacgtca gcaagaagcg cagagctggc agcaacgcca
    25981 gaacgaatta accgcgctgc aaaaccgtat tcagcagctg acgccgattc tggaaacgtt
    26041 gccgcaaagt gatgatctcc cgcacagcga agaaactgtg gcgctggata actggcggca
    26101 ggttcatgaa caatgtctcg cattacacag ccagcagcag acgttacagc aacaggatgt
    26161 tctggcggcg caaagtctgc aaaaagccca ggcgcagttt gacaccgcgc tacaggccag
    26221 cgtctttgac gatcagcagg cgttccttgc ggcgctaatg gatgaacaaa cactaacgca
    26281 gctggaacag ctcaagcaga atctggaaaa ccagcgccgt caggcgcaaa cgctggtcac
    26341 tcagacagca gaaacgctgg cacagcatca acaacaccga cctgacgggt tggctctcac
    26401 tgtgacggtg gagcagattc agcaagagtt agcgcaaact caccaaaagt tgcgtgaaaa
    26461 caccacgagt caaggcgaga ttcgccagca gctgaagcag gatgcagata accgtcagca
    26521 acaacaaacc ttactgcagc aaattgctca aatgacgcag caggttgagg actggggata
    26581 tctgaattcg ctaataggtt ccaaagaggg cgataaattc cgcaagtttg cccaggggct
    26641 gacgctggat aatttagtcc atctcgctaa tcagcaactt acccggctgc acgggcgcta
    26701 tctgttacag cgcaaagcca gcgaggcgct ggaagtcgag gttgttgata cctggcaggc
    26761 agatgcggta cgcgataccc gtaccctttc cggcggcgaa agtttcctcg ttagtctggc
    26821 gctggcgctg gcgctttcgg atctggtcag ccataaaaca cgtattgact cgctgttcct
    26881 tgatgaaggt tttggcacgc tggatagcga aacgctggat accgcccttg atgcgctgga
    26941 tgccctgaac gccagtggca aaaccatcgg tgtgattagc cacgttgaag cgatgaaaga
    27001 gcgtattcct gtgcagatca aagtgaagaa gatcaacggc ctgggctaca gcaaactgga
    27061 aagtacgttt gcagtgaaat aactattcag caggataatg aatacagagg ggcgaattat
    27121 ctcttggcct tgctggtcgt tatcctgcaa gctatcactt tattggctac ggtgattggt
    27181 agccgttctg gtagttgtga tggtggtatg aaaaaagtca ttttatcttt ggctctgggc
    27241 acgtttggtt tggggatggc cgaatttggc attatgggcg tgctcacgga gctggcgcat
    27301 aacgtaggaa tttcgattcc tgctgccggg catatgatct cgtattatgc gctgggggtg
    27361 gtggtcggtg cgccaataat cgcactcttt tccagccgct actcactcaa acatatcttg
    27421 ttgtttctgg tggcgttgtg cgtcattggc aacgccatgt tcacgctctc ttcgtcgtac
    27481 ctgatgcttg ccattggtcg cctggtttcc ggctttccgc atggcgcatt ttttggcgtc
    27541 ggagcgatcg tgttatcaaa aattatcaaa cccggaaaag tcaccgccgc cgtggcgggg
    27601 atggtttccg ggatgacagt cgccaatttg ctgggcattc cgctgggaac gtatttaagt
    27661 caggaattta gctggcgtta caccttttta ttgatcgctg tttttaatat tgcggtgatg
    27721 gcatcggtct atttttgggt gccggatatt cgcgacgagg cgaaaggaaa gctgcgcgaa
    27781 caatttcact ttttgcgcag cccggccccg tggttaattt tcgccgccac catgtttggc
    27841 aacgcaggtg tgtttgcctg gttcagctac gtaaagccat acatgatgtt tatttccggt
    27901 ttttcggaaa cggcgatgac ctttattatg atgttagttg ggctagggat ggtgctggga
    27961 aatatgctaa gtggcaggat ttcaggacgt tattcaccac tgcgcattgc agcagtgact
    28021 gactttataa ttgtactggc actgctgatg ctctttttct gcggcggcat gaaaacaacg
    28081 tcgcttattt ttgcttttat ttgttgcgcg ggattatttg ccctttcagc acctctgcaa
    28141 atattgttac tgcaaaatgc caaaggcgga gagttattag gtgccgcagg tgggcaaata
    28201 gcgtttaacc tcggtagcgc cgtcggcgca tattgcggag gtatgatgct gacgctgggg
    28261 ctggcatata attacgtggc gctgcctgcc gccctgcttt cgtttgctgc gatgtcatcg
    28321 ttgctgctgt atggtcgcta taagcgccag caagcggcgg atagtccggt gctggcgaaa
    28381 ccactggggt agattactgt cacggtgtgc gttgcttatt gccggatgcg gcgtgaacgc
    28441 cttatccgcc ctacgcggtt ctggcacatt ttgtaggcct gataagacgc ggcaagcgtc
    28501 gcatcaggca tgatgcgcca attgcctggg ttttttactc ttgtggccat aaccacgcag
    28561 cgccgcgtac gccgctggaa tcaccgtgct tcgccttacg caccggcgtt tcacattcgc
    28621 cgccgaagac aaattgttta atcaactgcg gaaccgtttg atataaacgg tctacattgc
    28681 tcatcccgcc ccccaggaca atcacatccg gatcgagaat attcacgaca tgtgccagcg
    28741 attttgccag ccgcagctcg tagcgacgca atgccagttc cgctaccgga tcgctttctt
    28801 caaccaggcg gataatttca ctgcctttca gcgcatgtcc gctcaaacga cgataatccg
    28861 tcgcgaatcc cgtgcccgaa ataaaggttt caatacaacc ttgtttaccg caataacaag
    28921 ggacttcctc gcgataacgc agttcgtctt cgtccatcca cggtagcgga ttgtgtcccc
    28981 actcacctgc cgtgccattg ccgccgatat gcgcccgccc attgaatgcc acgcccgcgc
    29041 cgcatcccgt gccgataatc acggcaaata ccgtctgcgc tcccgctgcc gcgccatcta
    29101 ctgcttctga aaccgccaga cagttggcgt catttgccag ccgcacttcc cgctgcaacc
    29161 tcgcgcttaa gtctttatcg aatggctgac cgttgagcca ggttgaattg gcattcttca
    29221 ccacaccggt gtaaggcgaa attgagccag gaatgcccat acctaccgtt ccgcgctgcc
    29281 ccgtcgcctg ctccgccata tcaaccaacg tggcgatcgt ttcaatagtc tgccggtaat
    29341 catcacgcgg cgtgggcaga cgatggcggt acaactgctc ccctgcatcg cccagtgcaa
    29401 tcacttcagt tttggtgccg cctaaatcga tacctatacg cacggtactc tccttatttt
    29461 tttcaatatc aatagcgtag agacggacaa ccggattggc aatgcaaggc cgccgacaat
    29521 tcgttatcat gcccgctaaa tttaacgaca aggccgtgga aattatcatg ctgtggttca
    29581 aaaatttaat ggtttaccgt cttagccgcg agatttcgct gcgtgcagaa gagatggaaa
    29641 aacagctagc ctcgatggca tttaccccat gcggcagcca ggacatggcg aagatgggct
    29701 gggttcctcc gatgggatcg cacagcgatg cgttaacgca cgttgccaat ggtcaaattg
    29761 ttatctgcgc gcgcaaagaa gaaaaaatcc taccgtctcc ggtgattaaa caggcgctgg
    29821 aagcgaaaat cgccaaactg gaagcggaac aggcgcgtaa gctgaagaaa accgaaaaag
    29881 attcgctgaa agacgaagtg ctgcactctc tgttgccgcg tgctttcagc cgttttagcc
    29941 agacaatgat gtggatcgac acggttaacg gtttgattat ggtggactgc gccagtgcca
    30001 aaaaagcgga agatacgctg gcactgctgc gtaaaagcct ggggtcgtta ccggttgtac
    30061 cattgagcat ggcaaacccg attgaactga cgctgaccga atgggtacgt tccggtagtg
    30121 cggcacaggg cttccagctg cttgatgaag ccgagctgaa atcgttgctg gaagatggcg
    30181 gagtgatccg cgcgaagaaa caagatctga ccagcgaaga gatcaccaat cacattgaag
    30241 ccggaaaagt ggtgactaaa ctggcgctcg actggcagca gcgcattcag tttgtgatgt
    30301 gcgacgatgg ttcgctcaag cgtctgaagt tctgcgacga gctgcgcgat caaaacgaag
    30361 atatcgaccg tgaagatttc gcccagcgtt ttgatgccga tttcatcctg atgactggtg
    30421 aactggcagc gttaattcaa aacctgattg aaggattagg tggcgaagca caacgttaat
    30481 tgctgatttt cctttaatgc cggatgcgac gcctgccgcg tcttatccgg cgtacgaagc
    30541 cacaccaggc atataattat tcgctacggc gggcaataat tttttagcgc agcaatatta
    30601 tgcgttttac gccgtaactg atctaatcgc ctcatcccta ttcgcctgtc agcagcacgc
    30661 agaatattca ctaaccccca tttatcgctg gtaaattgtt ttgttaacaa ctccgctttt
    30721 ggggtgtcaa tatattcacg caataaatca gaaattgaac aaatatccgt caggtaagga
    30781 taccagcaag tttcaccgtg aaaattacgc actccaccgt tgcctgcaat aaagtctttc
    30841 gggtaatccc agatcacgtt tttatccaac gtgatccaat aacgcggtaa atcggtgctt
    30901 ccaccatttt ggctgcgcat gggataacgg gtacagtgaa tttgcaggtt gatcgttggc
    30961 gtcagtaagt catagatctc ccgctgtaac ttgctccagg ggcgttgtgt cattgttttt
    31021 cctcaagccg gtgacgcgct tcattgcgaa ttttccgcgt tgccccggtt ttcactaaat
    31081 cccgtaactg cgaaagatcg ggtttatcga caaataaaag taatgatgga tcctctttta
    31141 gccacaccgt tttcacatcg gcagccagct gtggattatt aatagccagc gaccgatcct
    31201 gagactcagt caatgtcgtt aacaacgacg ccggggtatg tgcgttgcgt aatgccgttt
    31261 ggcgcaaatg atcattgcga ctttgcagca atgcacgcca ccattcatca tctgatttat
    31321 cttttatcgt cgccagttcc gcaaaaatat cgctatcctc atggttccag cggtgtaatc
    31381 ctctttcact ttgtctacgg gtgctatttt tcatactgac aacagccagc gcccagtcag
    31441 agttgaagcg atactgttga tcgagtttgt tgataattcc ggaaggtaat tctttattat
    31501 caaataattg gttacgcatt cgatagcggt attcagcggg taaatcaacc agtaaatcaa
    31561 tggcattatt taacatctct tcgtcaacaa caccattatc aatcagcgcc agctgtactt
    31621 cgtaggcttg ctcttgatct tcttgccgtt tgctcatcat taaatttaat gtgcgttgcc
    31681 ataactgttt cttatattcc agcggcaata atttatcgcg tgccagctca tcccagacgg
    31741 tgggaacatc atgctgagtc aatagataac taatcacgtc cttcgatgtc agataacgcg
    31801 caccttcgcg caaattgtgc acatcttctt ttgccagaga cagttgacgc atctccggca
    31861 acgctatcaa taacaccttt acgatatttt tattttcttt attatccaga tacatttgtt
    31921 ctgcgatacg ttcaatatcc tctgtacgca gggttgctga cagttgggta atttttaatt
    31981 cagtaagcgt ccgagctaat gcttcacgaa ctttcagtga tttatcttta ctcaacatca
    32041 ctgcttgttg tgcggttaaa tctgctccgg taattctttc acgtaactcc gggtcttcgt
    32101 tatttatcaa ctcatcggcc actgcagtcg gaaccagcca ggcgtaatcg taatggctga
    32161 gtaatgtctt gcgtacactg agaaaggggc tatgacttaa cgacgcaaca atatctggag
    32221 aaagattatc ctgtctggcc tgctccagct gtacgctctc gacgggtaca gacattaatt
    32281 ttttattcac atcggcggaa atattactgt ttttaaccag ccctctggca acagcctcat
    32341 ctttaattaa ttcatcggta agccaatgca tctgttgagc gggccaggac tgcgccatcg
    32401 attcacgcgt ctggtcatcg gggtgctgta acagttgcag cataacggcg ggtgataaac
    32461 tctctgtatg agtcagaaaa cgtgccacca gtggatcttg atgctttatt ttattcgctg
    32521 ggaaattatc gagtttcccc gtttcaatcc attttaattt ctcattaatt tcaggagcgg
    32581 ctttatctcg aaaaagcggt aaaccctgaa cgatgttgtc tgcgacgcta tcatagtcag
    32641 gatacgcatt ggaaatgtta tcggtttcga tttcagtgtg atcataaaga tcggcagtca
    32701 ccagtgaata ggctttttga atattctcat cccagttata tccttcacca cttccatctt
    32761 cattgatata aaattcagct ttaatgactt caaattgcgt tgctttatcg gaaacaataa
    32821 tgcctcgcgc actaattccc ttcatcactt caaagttatg atcattgtaa tcagcataaa
    32881 ctaaaccttt cgctgtcatc tgtcctttga catacgcgaa atcatgatta ataaagttat
    32941 cgacaataac attacctaat acaatcagat gaccattacc tgacagataa tcatcataat
    33001 cgccggaaat cgttaaatta ccgttcacca ccagtttatt tttattgctg aagttagcgg
    33061 gtaatgatag gtcaccatta tgataatacc agcggatatt atcttttagc atataagcag
    33121 cttccatact atcgtcggtt gccgctttaa ccacgttgcg ctcatctgcc gtcataagat
    33181 gaaagtcaga ggtgataagg tcctcgctac tgatccactc tgtggcacat gcattgccag
    33241 tcataaaaaa taagaccata cagatgacca atatattaat tctattcata atcttcaatg
    33301 atatttatat caaccttgat gaattatgaa tatatcggaa attgaaaaaa tcgcagcaaa
    33361 ggaaatatta ttatggggtt atgagaggac atttcagaaa aatgccctcc cgttcaagcg
    33421 gaaggcgagg aattacagat agcggcacag ataagaggtg ggttcggcaa cttgcagatg
    33481 aaactcactg tgaccgggaa cattaaacac cgaaccggct tcatacactt gccagtcggt
    33541 cgcgtcaggc agtaacacat tcagcgcgcc actgattacc gtcatctctt ccggctcagc
    33601 ggtgctgaag gtgtattcgc cttcaaccat aacacccacg ctggcgcgac cagtgctgct
    33661 gctggaaaag ccgatcgatt tcactttgcc ggaaaagtac tcattacttt gaagcataaa
    33721 ctggccctgt ctggtagttg aatatctact tcactatagg ggccagatgc gctggctgtc
    33781 acgcaaaatt acaccagtaa ttccgcagcc agccgtgcaa tcaatacgtt agagagcaag
    33841 acaggaacat cgagctgttt ttgcagtaaa tcgcgatgac gctggttaaa tcccaaacaa
    33901 tccagcatga tgacatctgc gcctttcgcc agtaattctt tcccggcatc aatgattttt
    33961 tgttctgaat catgaatggg gttacccaat gaaaatacag gcggtttctg caaaatttgc
    34021 catttttgcg cctgaacggt cagcaactcc tcaaccggta cgataacccc cacctgatga
    34081 tcttcaacaa tagaggaaac cagcggtggc aatatccgcg acggctcaag aaagatcgta
    34141 ttacgcgcag tcatactact aatgtttgcc gtactcatta atataatgac gtcgtagccc
    34201 tgattatcga gcacttcaac cacaccttgc aggtcacgct ctactttgcg acgcgaaaca
    34261 tgggccagat ggttgtcatt taataatgtg agaattgtgt cttcgcctgc ttctggcgcg
    34321 tactccgcca tcacttcttc gcggcttaac ttccccagca ggctatgatg ggaaatatta
    34381 tcttcgtcaa tgtattctgt caggagcggc aaaacttcct gcataggtac aatgccgatg
    34441 gttagaatcg ccaacgacgc actcattgtt cttccacctt acttttattt cgtcgttaat
    34501 taccgggcaa gtgttaagca ccactgctga cacttacttg tgctacaaat gacaaaaagc
    34561 gtagcagcag acgtgcacgg cataatgtaa agattccaaa tgattccagt aatggattgt
    34621 tattgtttaa tattctaatt atttgaaaaa catgaattat gaaaaatgtg acgcagatcc
    34681 cttgtagtgg cactcagaat cccttccgag cagtctgatg cggtcactct ttgtcagggt
    34741 cctcatagcg ttttttcgca tccatcgctt cctgagcggt cggatgttca ctgatcaacg
    34801 agtctggttt aggatgatcg gctctgagtt ggtaccaggt taccgtctgt cctggctttc
    34861 ctttttcaat tgtcactata tatgcttcac gaggataagg cggtttcgtt ggcatcgttc
    34921 ttccttattt cacgggatga acgttaagta taggcgctcg aaaatcaaca attgatcgtc
    34981 tgcgccaggg cgctgcgaat ttcagaaatc acctggctgg gttcgtttgt tgcgtcgatg
    35041 ataatatgcg caacttcgcg atatagcgca tcgcgttctt ccagcacttc ctgaacttct
    35101 tcgctcagcg gttttcccgt taaggttggc cgtaaatctt cttccggtgc agcttgcagt
    35161 cggttaacca ggactgatac tggcgcacac aaataaacca cgatcccgtt attttgcatg
    35221 aagtgacgat taaattccgt cagaataatg ccgccgcctg tagcgataac ggtggatggc
    35281 gcagttaccg cttccagcgc cgccgtttct ctggcgcgaa atcccgccca ctcttccctt
    35341 tcgacgatct ccgcgaccgt catattgagc tgtgattgca accactgatc ggtatcgaca
    35401 aaccgacggt taagcgaatc ggcaagggcc attccgaccg ttgttttacc acagccccga
    35461 ggcccgatca gaaaaagagg ttgtgtcatc gtgggttttc cccaataggt cgcaatgcgg
    35521 cgaaagccgg tgtcatgaga atagcgatca taccatcaaa ctagtacaat ttcgattgta
    35581 aagaaaaaat tccacttaaa gtgaaaatct caatacatcc cttactatac caataaatat
    35641 tcaagaatga agtgtaaata ataaattaca tttagccacg actacgttgc acttccagcc
    35701 accacttctc cagctccgcg gcaaaggcct ggcggtcacg ttgtgaaagg ctatctggtc
    35761 cgccggtctg gatcccactg gcacgtaagg tatccataaa atcgcgcatc gtcaggcgct
    35821 cacgaatggt cgctggcgtg taacgttcac cgcgcggatt aagcgccgca gcgcctttct
    35881 cgatggcttc agcagccaaa ggtatatctg cggtgatcac caaatctccc gcttcacact
    35941 gccggacaat ttcgttatcg gcaacgtcga aacctgccgc gacgcgcagc gtacgaataa
    36001 atcgcgatgg cggcacgcgt aaactctggt ttgctaccag taccagcggc atctgcatac
    36061 gttccgccgc gcgatacaaa atctctttaa ttacattggg acacgcgtcg gcatccaccc
    36121 aaattgtcat aaagtcatcc tttgttgggt aatcctctat tgtgtcgcgc ttttgccttc
    36181 cggcatagtt ctgtttatgc ttctgccagc gattatcaaa acaatgaatt tcacggcagg
    36241 agtgaggcaa tggaaaagaa aatcggtttt attggctgcg gcaatatggg aaaagccatt
    36301 ctcggcggtc tgattgccag cggtcaggtg cttccagggc aaatctgggt atataccccc
    36361 tccccggata aagtcgccgc cctgcatgac aagttcggca tcaacgccgc agaatcggcg
    36421 caagaagtgg cgcaaatcgc cgacatcatt tttgccgccg ttaaacctgg catcatgatt
    36481 aaagtgctta gcgaaatcac ctccagcctc aataaagact ctctggtcgt ttctattgct
    36541 gcaggtgtca cgctcgacca gcttgcccgc gcgctgggcc atgaccggaa aattatccgc
    36601 gccatgccga acactcccgc gctggttaat gccgggatga cctccgtaac gccaaacgcg
    36661 ctggtaaccc cagaagatac cgctgatgtg ctgaatattt tccgctgctt tggcgaagcg
    36721 gaagtaattg ctgagccgat gatccacccg gtggtcggtg tgagcggttc ttcgccagcc
    36781 tacgtcttta tgtttatcga agcgatggcc gacgccgccg tgctgggcgg gatgccacgc
    36841 gcccaggcgt ataaatttgc cgctcaggcg gtaatgggtt ccgcaaaaat ggtgctggaa
    36901 acgggagaac atccgggggc actgaaagat atggtctgct caccgggagg caccaccatt
    36961 gaagcggtac gcgtactgga agagaaaggc ttccgtgccg cagtgatcga agcgatgacg
    37021 aagtgtatgg aaaaatcaga aaaactcagc aaatcctgat gactttcgcc ggacgtcagg
    37081 ccgccacttc ggtgcggtta cgtccggctt tctttgcttt gtagagcgcc aaatctgccg
    37141 atttcaacca ctcgcgatag tgactcattt gtgggttcag cggcgcaacc cccacactaa
    37201 tccgtaaatt tacctgtggc gtattcggca aacgtaatgt atttagccct tcatgcaccc
    37261 gtaacatggc ggtaatggcg ctctcagctg gcgtaccgga catgattacc gcaaactcat
    37321 cgccgccaaa ccgaccaatc acatcgctac cgcgcagggt aatttgtaac tgtcgggtaa
    37381 gcgccacaat cgcttcatcg cccacatcat ggccccaggt atcgttgatg ctcttgaaat
    37441 ggtcgatatc gataatcagt aacgttgcat cgcgattatg ccgccgacag ttatcaaatt
    37501 cattgcgtaa catagtttcc caatgacgtc ggttatacac gcccgtcatg ccgtcgcggg
    37561 tactcatgac ctgcaacctg cgtttatgtt ccgccagttt ggttgccgtc tggtagctga
    37621 cccagccaaa cagcagagga taaatgacaa taatgggaag ggagagccac cattccagcg
    37681 gcgcactatt gaacgacacg gtaatgcccg tcagctcgag ggtgacaagg caggaaacca
    37741 ccatcaacac cagacccgcg acaaacagac gggggccgcc tgcccccatc aaattcagac
    37801 acataatcat caacatcgcg gtggaaggca gcacgtttac gcccattacg cctacccata
    37861 ttcccgctaa tactgcatcg gtttttaagt tgtaaatttc ccggctaagc ggatcgacgg
    37921 ccctgctcgc tatctgccag gctaaatgcg gccagacgaa cgcccagccg accaacacca
    37981 gccaccacca gcccggcggc gggtgtgaaa ccagcgttga agcaatcggt aagaacatgc
    38041 cagccaggcc aaccgcacgg ggtagtctga cgcgacgggc gaagcgcagc ccggaccgct
    38101 gatgttcgtt ttgaggagtt aaaggaggtt cctccccgtg cgccgccgct tttttgaaaa
    38161 agttttcatc attcattatt tttgggaaca ttcagaaaca attttcccaa attatagaga
    38221 cggggctaaa ggcagaagta tgaaatttcg ggtgagccat tttagtagct cacccgttag
    38281 cactatcagg tatcaggcgg ctttcttgag gcaggcgctc ataaacttat tacgatcatc
    38341 acctttcagt gattgttgcg ttgcttgatt attacattcg cgcatctttt gctgctgtgg
    38401 cgtcaaactt ttttcaccag gcgcagactt gctgttcttc aggcaatcac tcatgtaggt
    38461 cttacgagca tcccctttca acgcctgtgc cgtcgcctgc tgattacagg aggtcatacg
    38521 ctgttgttgt ggggttaagg ttctctcggc agcgccgacg gtggttaaaa aaaccagacc
    38581 gaaaagcaag gtaaccagta atgtaatttt catagcacca tccctcttca tgttttaacc
    38641 atgagcgtat gcgcccgtga tctgacatta agtctggttg ctaacagcaa aaaaaccacc
    38701 cggcagcgaa aattcactgc cgggagcggt tttatttcag ccccagagcg gctttcatgg
    38761 tgtagaagag atcggtctgg tcggtcagtc caacgacatt ggcggcatgc gggccatacg
    38821 ccgcaatacg caactgactg ccggtatgtt cttgtgaatc ctcttcggag ttcccgtaac
    38881 tcatcaccat cactgcgcca tctttggtat ttagcgcctg agtgaggccc ggagctttgg
    38941 tatccggcgc aacaatctgg ctggcgtggg cgtgatcagc ggtgactatg accagcgtgt
    39001 taccatcctt tttggcgaat tccagcgccc gttgtacggc ttcatcgaga tcgaccgtct
    39061 cgccaatttg cccacaagga ttcgcagcat gatcctgttt atcgattgac gcaccttcaa
    39121 cttgcaggaa aaagcctttc tcatttttac tcaacaattc aatggctttg tcggtcatct
    39181 gtgccagggt cggtacactg tcattacgtt gcggattagg cgtacaggtg actgcgggct
    39241 tatcgatatt accgtggtac gttgctttcg gtcctagcca gcgcactggc atattgccgt
    39301 cagcaaacag gccaagcagg ggtttttgct gattcgcttc cgtcaccgaa ttcagtgagg
    39361 cagcatcgct caccaactga taaccacgcg cctgtgcctg ttcacgcagc gtttttccct
    39421 gccattcacc agcggttgcc gtttcagcaa aggtttttgc gccgccgcca agcgtaacgt
    39481 cggcacgagc gttaagcagc tgttcggtaa tcgatccttt tccgcctttt tccagagcgt
    39541 tacccggaca tttttcactg gtcgcgctcg gaccgtagca tttgcgcgag gtcacatgcg
    39601 ccaccagcgc ggcgggcgtg gcatcctgca actctgcggt agaaacgtta ccggtcgcca
    39661 gacctgcggc ttttgccatt tccagaatcg ttgggtgatc tttttcgtga atatcgacgc
    39721 ccagcgcgcc gttataggtt ttgacaccgg ttgaccaggc ggttgctgat gcagccgagt
    39781 cggtgacgta gtccggtttt ccggtttttt tattcagcgc atagtgagtg tattgcccgg
    39841 taagcggtaa ggcatcgata cctttaaaaa agccgcccgc accttcggca taattacgtg
    39901 cggcagtaat ttccgaatcc cccatcccat cgccaatcag caaaataata ttttttgcag
    39961 gtttatcgct aagagaatca cgcagagcgg cggtctgatc agccgttaag cggcgagcac
    40021 cgccgggtgc agtaatatcg ccctgagcag cccggttttc cagaacaggc atttctggtg
    40081 tccgggcttt tgtcacaggg gtaaacagta acggtaagag tgccagtgca atagtgcttt
    40141 gtttcacttt attttctcca tgtacaaata cattaaaaaa taaaaacaaa gcgactataa
    40201 gtctcggccg tgacaacttt atgacagctg ttgaaaagat taacttttta ctgacgagga
    40261 tgcttcaata acttctttac gtaatcgcgc agcagctccg tatcgtcgtc aggaatgctg
    40321 gcatcgggct ttacctcgta cagcgccccc tctacctgat caatcaaccg ctgttggtca
    40381 ttttgcgcca tattgcgaag cattgcagtg acgataatct ccaaagcttc tacctgcgca
    40441 cacagttctt tcgactcttc ttctttttgg gcaagcttaa ataacaactc agcaatgaga
    40501 tttttcatgt ctgtatttcc ttatccaaag tatggagaag ttaacattat gtttcacaac
    40561 ttcatagtgt ctggaggtat tatttttatg gcaaaggaaa tactttgcgc aaatattttt
    40621 tgtttgatta ccagcgaaac gtttctctta tttaaataat ta
//
LOCUS       DAZKIP010000027        39448 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZKIP010000027 DAZKIP010000000
VERSION     DAZKIP010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.027"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            393..1652
                     /gene="codB"
                     /locus_tag="ACHOUK_001704"
     CDS             393..1652
                     /gene="codB"
                     /locus_tag="ACHOUK_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFP9897174.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSLIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
     gene            1642..2925
                     /gene="codA"
                     /locus_tag="ACHOUK_001705"
     CDS             1642..2925
                     /gene="codA"
                     /locus_tag="ACHOUK_001705"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HFP9897175.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKLEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYSIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(3058..3957)
                     /gene="cynR"
                     /locus_tag="ACHOUK_001706"
     CDS             complement(3058..3957)
                     /gene="cynR"
                     /locus_tag="ACHOUK_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HFP9897176.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIHLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHLPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAIPLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECADVGENESR"
     gene            4067..4726
                     /gene="cynT"
                     /locus_tag="ACHOUK_001707"
     CDS             4067..4726
                     /gene="cynT"
                     /locus_tag="ACHOUK_001707"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HFP9897177.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            4757..5227
                     /gene="cynS"
                     /locus_tag="ACHOUK_001708"
     CDS             4757..5227
                     /gene="cynS"
                     /locus_tag="ACHOUK_001708"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HFP9897178.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKALSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARQVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            5233..6414
                     /gene="cynX"
                     /locus_tag="ACHOUK_001709"
     CDS             5233..6414
                     /gene="cynX"
                     /locus_tag="ACHOUK_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HFP9897179.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAALTPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSTREVASSHKTTTIPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LALQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSVQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     A"
     gene            complement(6517..7128)
                     /gene="lacA"
                     /locus_tag="ACHOUK_001710"
     CDS             complement(6517..7128)
                     /gene="lacA"
                     /locus_tag="ACHOUK_001710"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HFP9897180.1"
                     /translation="MNMSMTERIKAGKLFTDMCEGLPEKRLRRKTLMYEFNHSHPSEV
                     DKRESLIKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSIVTKDIPPNVVAAGVPCRVIREINDRDKQYYFRDYKVESSV"
     gene            complement(7194..8447)
                     /gene="lacY"
                     /locus_tag="ACHOUK_001711"
     CDS             complement(7194..8447)
                     /gene="lacY"
                     /locus_tag="ACHOUK_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HFP9897181.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAILLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            complement(8499..11573)
                     /gene="lacZ"
                     /locus_tag="ACHOUK_001712"
     CDS             complement(8499..11573)
                     /gene="lacZ"
                     /locus_tag="ACHOUK_001712"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFP9897182.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TNRPSQQLRSLNGEWQFVWFPAPEAVPESWLECDLPDADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLLSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATLFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLGLNVENPKLWSAEIPNIYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTSATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGEMRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDLVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGRTIEVTSEYLFRHSDNELLHWTVALDGKPLASGEVPLDVAPQGKQVIE
                     LPELPRLESTGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSAPHAIPQ
                     LTTSETDFCIELDNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTVHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            complement(11696..12778)
                     /gene="lacI"
                     /locus_tag="ACHOUK_001713"
     CDS             complement(11696..12778)
                     /gene="lacI"
                     /locus_tag="ACHOUK_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFP9897183.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPKT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            complement(12815..13768)
                     /locus_tag="ACHOUK_001714"
     CDS             complement(12815..13768)
                     /locus_tag="ACHOUK_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HFP9897184.1"
                     /translation="MRPLTVAIIAVAGFSPFHLSVPFIVFSEKMAEKKRFHVIICAEK
                     PGNVDSADGFSVTATHDYTAVIQADIVIIPYWGTITQKPPQKLLEALTTARDNGAQIV
                     GLCLGTFVLGYAGLLKNKRAATHWEFEREFQARFPQTHLDINALYVDDDGIITSAGTA
                     AALDCCLYIVRQHFGSDYANHIARRMVVPPYRTGGQAQFIEQPVPKNTHDERINLLLD
                     YLRQNIAQQHDLDSLAQRVMMSRRTLTRHFMKATGSSIAEWLITERLRRSQELLGSSQ
                     LPVERIAAEVGFLSPVTWRQHFKSHFGVSPAEWRKTFRGMA"
     gene            complement(13788..14564)
                     /locus_tag="ACHOUK_001715"
     CDS             complement(13788..14564)
                     /locus_tag="ACHOUK_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFP9897185.1"
                     /translation="MKITQIRNATQIIQYAGKTFLIDPLLAPKGSYPGFAGTARAEIR
                     NPTVELPCDLTTLLNVDALIVTHLHEDHWDETASRVVPKDKLIYVQNEQDAESLNKQG
                     FTHLTVLTDETKFGDITLHKTTGQHGSDRTYADPAMAKRLGAACGVVFQHPTEKTLYL
                     AGDTLWRDDVEATMLTFQPEVVVLNAGFAHVLGHGAIIMGAEDILKTHFTLPEAQIIA
                     THMEAVNHCLLTRDALKEYARDNQIIEFVNVPEDGETLTF"
     gene            complement(14679..15512)
                     /gene="mhpR"
                     /locus_tag="ACHOUK_001716"
     CDS             complement(14679..15512)
                     /gene="mhpR"
                     /locus_tag="ACHOUK_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFP9897186.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQE
                     RKELIEMLASRPGDDYQLAREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            15703..17367
                     /gene="mhpA"
                     /locus_tag="ACHOUK_001717"
     CDS             15703..17367
                     /gene="mhpA"
                     /locus_tag="ACHOUK_001717"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFP9897187.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDNVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGVSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     LREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALSTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            17369..18313
                     /gene="mhpB"
                     /locus_tag="ACHOUK_001718"
     CDS             17369..18313
                     /gene="mhpB"
                     /locus_tag="ACHOUK_001718"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFP9897188.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            18331..19197
                     /gene="mhpC"
                     /locus_tag="ACHOUK_001719"
     CDS             18331..19197
                     /gene="mhpC"
                     /locus_tag="ACHOUK_001719"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308431.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFP9897189.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARA"
     gene            19207..20016
                     /gene="mhpD"
                     /locus_tag="ACHOUK_001720"
     CDS             19207..20016
                     /gene="mhpD"
                     /locus_tag="ACHOUK_001720"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFP9897190.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAAAFSSAAPKGSLS"
     gene            20013..20963
                     /gene="mhpF"
                     /locus_tag="ACHOUK_001721"
     CDS             20013..20963
                     /gene="mhpF"
                     /locus_tag="ACHOUK_001721"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFP9897191.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHDQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEENVDQLNVNMVTCGGQATIPMVAAVSRVVRVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            20960..21973
                     /gene="mhpE"
                     /locus_tag="ACHOUK_001722"
     CDS             20960..21973
                     /gene="mhpE"
                     /locus_tag="ACHOUK_001722"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFP9897192.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAVDKLGWQHGADLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            22149..23360
                     /gene="mhpT"
                     /locus_tag="ACHOUK_001723"
     CDS             22149..23360
                     /gene="mhpT"
                     /locus_tag="ACHOUK_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFP9897193.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRLRMQPCADA"
     gene            23462..24001
                     /gene="yaiL"
                     /locus_tag="ACHOUK_001724"
     CDS             23462..24001
                     /gene="yaiL"
                     /locus_tag="ACHOUK_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFP9897194.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(24126..24959)
                     /gene="fghA"
                     /locus_tag="ACHOUK_001725"
     CDS             complement(24126..24959)
                     /gene="fghA"
                     /locus_tag="ACHOUK_001725"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFP9897195.1"
                     /translation="MELIEKHASFGGWQNVYRHYSASLKCEMNVGVYLPPKAAGEKLP
                     VLYWLSGLTCNEQNFITKSGVQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMNHFPATAKKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKEAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTQNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(25052..26161)
                     /gene="frmA"
                     /locus_tag="ACHOUK_001726"
     CDS             complement(25052..26161)
                     /gene="frmA"
                     /locus_tag="ACHOUK_001726"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFP9897196.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            complement(26196..26471)
                     /gene="frmR"
                     /locus_tag="ACHOUK_001727"
     CDS             complement(26196..26471)
                     /gene="frmR"
                     /locus_tag="ACHOUK_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFP9897197.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            complement(26631..27677)
                     /gene="fbpC"
                     /locus_tag="ACHOUK_001728"
     CDS             complement(26631..27677)
                     /gene="fbpC"
                     /locus_tag="ACHOUK_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric ABC transporter ATP-binding protein"
                     /protein_id="HFP9897198.1"
                     /translation="MSQKNFVELRNVTKRFGSNTVIDNINLTIPQGQMVTLLGPSGCG
                     KTTILRLVAGLEKPSEGQIFIDGEDVTHRSIQQRDICMVFQSYALFPHMSLGENVGYG
                     LKMLGVSRSEVKQRVKEALAMVDLEGFEDRYVDQISGGQQQRVALARALILKPKVLLF
                     DEPLSNLDANLRRSMRDKIRELQKQFNITSLYVTHDQSEAFAVSDTVLVMNKGHIMQI
                     GSPQDLYRQPASRFMASFMGDANLFPANFSEEYVDIYGYRLPRAAHFPVQGSGTVGVR
                     PEAITLSNHGEESQRCVIRHVAYMGPQYEVTVEWHGQEILLQVNATRLQPDIGEHYYL
                     EIHPYGMFVLADAA"
     gene            complement(27689..29767)
                     /locus_tag="ACHOUK_001729"
     CDS             complement(27689..29767)
                     /locus_tag="ACHOUK_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9897199.1"
                     /translation="MSQTLALHPVKKRDAIFLWVLLGWLAFVLLPSWSLDYGLLESTS
                     DEILGAYGWSHFNISWLWYLLPSLLLVRPFRQATREQRSRHYFDAGWALLCMAFIVIS
                     ATVAGRGLGYAAIVLFVELGAIMTLALTRLEWLGGDRFVIGSLIAIVALIGIFIVWPS
                     IAIFIPMFTNDAGELAPLAFMTVLSQAHIVQVIVNSIVLSIAVGIGCAFFGLVLAIYT
                     TRIAKRSAIIGRIFSILPIVTPPFVVGLGVTLMMGRSGYVTEFMVEWFGLTNTNWLYG
                     FTGIWLAQVLAFTPMAFMILDGAIKTIHPSLEEASYTLRASRWQTFNGVFVPLLKPAL
                     ANAFLIVVVQSLADFSNPLVLGGNFDVLATQIYFYITGSQLDYQAASTLGAFLLLFSL
                     LVFCIQYMWIGKRSYVTVSGKSYRGDVQPLPVTLVWSVVAILAIWIAFNALLYGSIFY
                     GSFTVNWGVDYTLTLDNFIKLFGQGMSDGAWPSLLDTLLYAGIAAPITAIFGLLIAWV
                     VVRQQFKGKKTIEFTTMLCFAVPGTVAGVSYILAFNSAPVYLTGTAAIVIISMVMRNV
                     PVGIRAGIAGLGQIDKSLDEASLSLRAGSLRTITHILLPLLRPAILSALIYSFVRAIT
                     TVSAIVFLVTPDTRVATAYILNRVEDGEYGVAIAYGSILIVVMLAIIFLFDWLIGEAR
                     ISRSKAKNQA"
     gene            complement(29836..30867)
                     /locus_tag="ACHOUK_001730"
     CDS             complement(29836..30867)
                     /locus_tag="ACHOUK_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9897200.1"
                     /translation="MKMTFTSTLIASAVALATLTGAAQAKGRLVVYCSATNEMCEAET
                     KAFGEKYDVKTSFIRNGSGSTLAKVDAEKKNPQADVWYGGTLDPQSQAGEMGLLQAYK
                     SPNLEQIMEKFRDPAKVKGNLSSAVYVGILGFGVNTDRLKEKNLPVPKCWKDLTKPEY
                     KGEIQIADPQSSGTAYTALATFAQLWGEDQAFDYLKQLNANVSQYTKSGIAPARNAAR
                     GETAIGIGFLHDYSLEKEQGAPLELISPCEGTGYEIGGVSILKGARNLDNAKLFVDWV
                     LSKEAQELAWKKGKSYQILTNTTAETSPNSLKLDDLKLINYDMDKYGSTDVRKALINK
                     WVSEVKMGK"
     gene            complement(30864..32180)
                     /gene="uhpC"
                     /locus_tag="ACHOUK_001731"
     CDS             complement(30864..32180)
                     /gene="uhpC"
                     /locus_tag="ACHOUK_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFP9897201.1"
                     /translation="METTMHARSAREINQCYRTLRPRLLMCMIIGYAAFYLTRKSVNY
                     VLPALQTDLGLDKGDIGLLGSLFYLSYGLSKFTAGLWHDSHGQRGFMGVGLFATGLLN
                     VVFAFGESLTLLLVVWTLNGFFQGWGWPPCARLLTHWYSRNERGFWWGCWNMSINIGG
                     AIIPLISAFAAHWWGWQAAMLTPGIISMALGIWLTLQLKGTPQEEGLPTVGHWRHDPL
                     ELRQEQQSPPMGLWQMLRTTMLQNPLIWLLGVSYVLVYVIRIALNDWGNIWLTESHGV
                     NLLSANATVMLFEVGGLLGALFAGWGSDLLFSGQRAPMILLFTLGLMVSVAALWLAPV
                     HHYALLAVCFFTVGFFVFGPQMLIGLAAVECGHKAAAGSITGFLGLFAYLGAALAGWP
                     LSLVIERYGWPGMFSLLSVAAVLMGLLLMPLLMAGITTTHARRIKQ"
     gene            complement(32253..33794)
                     /locus_tag="ACHOUK_001732"
     CDS             complement(32253..33794)
                     /locus_tag="ACHOUK_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFP9897202.1"
                     /translation="MSRNLRHWVILLFIVLAWGTGWLMLWTLSFYLTNNGQQAVLFLP
                     QGVYLALVILLSRRYWPALVLPPLLMMFWLHSEQLLNGYLMLATPVISLFPALLAQNF
                     WHRFPLYWQRLTLLLATVTAASLLNTALLSPFMSGPIMLPGLTSFTGGVLLTPFVYLI
                     FEFLRQQHRYQLLGLDTHNPPLRTSLIIWCSLFFIIGIGTQMVLSPEIERLLLIVVFL
                     PNVVMAWKFGWQGGVLSGLLGSMMITIARQIGVGFSNLVELEIFLATQALLGIGLGIA
                     ISRQQHLALNLHHYRQRLEAELAARRALAEKLIHTEEDTRKNLARELHDEIGQNITAI
                     QIQSQLVKRARDPAQIQSAASQINELARRIHLSTRQLLRQLRPPALDELTFREALLHL
                     INEFAFSERGIHCQFAYQLNSTPENETVRFTLYRLLQELLNNICKHAEASEVTIILRQ
                     QGEVLHLEVSDNGVGIASGKMAGFGIQGMRERVSALGGDLTLEKQHGTRVIVNLPTFL
                     QQIAG"
     gene            complement(33794..34423)
                     /locus_tag="ACHOUK_001733"
     CDS             complement(33794..34423)
                     /locus_tag="ACHOUK_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP9897203.1"
                     /translation="MIRVVLVDDHVVVRSGFAQLLSLEDDLEVIGQYSSAAQAWSALI
                     RDDVNVAVIDIAMPDENGLSLLKRLRAQKPQFRAIILSIYDAPTFVQSALDAGASGYL
                     TKRCGPEELVQAVRSVGLGGHYLCADAIRALRGGGQPAQALEILTPREREVFELLVKG
                     DSVKEIAFKLELSHKTVHVHRANVLGKLNCHSTIELVHFALDHHLLAGH"
     gene            34726..35688
                     /gene="tauA"
                     /locus_tag="ACHOUK_001734"
     CDS             34726..35688
                     /gene="tauA"
                     /locus_tag="ACHOUK_001734"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9897204.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIATPDVWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            35701..36468
                     /gene="tauB"
                     /locus_tag="ACHOUK_001735"
     CDS             35701..36468
                     /gene="tauB"
                     /locus_tag="ACHOUK_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFP9897205.1"
                     /translation="MLQISHLYADYGGKPVLEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            36465..37292
                     /gene="tauC"
                     /locus_tag="ACHOUK_001736"
     CDS             36465..37292
                     /gene="tauC"
                     /locus_tag="ACHOUK_001736"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFP9897206.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPIIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            37289..38140
                     /gene="tauD"
                     /locus_tag="ACHOUK_001737"
     CDS             37289..38140
                     /gene="tauD"
                     /locus_tag="ACHOUK_001737"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFP9897207.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWISGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(38247..39221)
                     /gene="hemB"
                     /locus_tag="ACHOUK_001738"
     CDS             complement(38247..39221)
                     /gene="hemB"
                     /locus_tag="ACHOUK_001738"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFP9897208.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         9085 a        10392 c        10822 g         9149 t
ORIGIN      
        1 cgacaccgtg ctcagtgcct gatgcgacgc tgatgcgtct tatcatgcct acaggcctga
       61 accgtaggtc ggataaggcg ctcgcgccgc atccgacacc gtgcgcagat gcctgatacg
      121 acgcaggcgc gtcttatcat gcctacaggc ctgaaccgta ggtcggataa ggcgctcgcg
      181 ccgcatccga caccgtgcgt agttgcctga tgcgacgctg acgcgtctta tcatgcctac
      241 ccactgtttt tacaccgata attttccccc cacctttttg cactcattca tataaaaaat
      301 atatttcccc acgaaaacga ttgcttttta tcttcagatg aatagaatgc ggcggatttt
      361 ttgggtttca aacagcaaaa agggggaatt tcgtgtcgca agataacaac tttagccagg
      421 ggccggtccc gcagtcggcg cggaaagggg tattggcatt gacgttcgtc atgctgggat
      481 taaccttctt ttccgccagt atgtggaccg gcggcactct cggaaccggt cttagctatc
      541 atgatttctt cctcgcagtt ctcatcggta atcttctcct cggtatttac acttcatttc
      601 tcggttacat tggcgcaaaa accggcctga ccactcatct tcttgctcgc ttctcgtttg
      661 gtgttaaagg ctcatggctg ccttcactgc tgctgggcgg cacccaggtt ggctggtttg
      721 gcgtcggtgt ggcgatgttt gctattccgg tgggcaaggc aaccgggctg gatattaatt
      781 tgctgattgc cgtttccggt ttactgatga ccgtcaccgt cttctttggc atttcggcgc
      841 tgacggttct ttcgttgatt gcggttccgg ctatcgcctg tcttggcggt tattccgtgt
      901 ggctggccgt taacggcatg ggcggcctgg acgccttaaa agcggtcgtt cccgcacaac
      961 cgttagattt caatgtcgcg ctggcgctgg ttgtggggtc atttatcagt gcgggcacgc
     1021 tcaccgctga ctttgtccgc tttggtcgca atgccaaact ggcggtgctg gtggcgatgg
     1081 tggccttttt cctcggcaac tcgttgatgt ttattttcgg tgcagcgggc gctgcggcac
     1141 tggggatggc ggatatctct gatgtgatga ttgctcaggg cctgctgttg cctgcgattg
     1201 tggtgctggg gctaaatatc tggaccacca acgataacgc gctctatgcg tcgggtttag
     1261 gtttcgccaa cattaccggg atgtcgagca aaaccctttc ggtaatcaac ggtattatcg
     1321 gtacggtctg tgcattatgg ctgtataaca attttgtcgg ctggttgacc ttcctttcgg
     1381 cagctattcc tccagtgggt ggcgtgatca ttgccgacta tctgatgaac cgtcgccgct
     1441 atgagcactt tgcgaccacg cgtatgatga gtgtcaattg ggtggcgatt ctggcggtcg
     1501 ccctggggat tgctgcaggc cactggttac cgggaattgt tccggtcaac gcggtattag
     1561 gtggcgcgct gagctatctg atccttaacc cgattttgaa tcgtaaaacg acagcagcaa
     1621 tgacgcatgt ggaggctaac agtgtcgaat aacgctttac aaacaattat taacgcccgg
     1681 ttgccaggca aagaggggct gtggcagatt catctgcagg acggaaaaat cagcgccatt
     1741 gatgcgcaat ccggcgtgat gcccataact gaaaacagcc tggatgccga acaaggttta
     1801 gttataccgc cgtttgtgga gccacatatt cacctggaca ccacgcaaac cgccggacaa
     1861 ccgaactgga atcagtccgg cacgctgttt gaaggcattg aacgctgggc cgagcgcaaa
     1921 gcgttattaa cccatgacga tgtgaaacaa cgcgcatggc aaacgctgaa atggcagatt
     1981 gccaacggca ttcagcatgt gcgtacccat gtcgatgttt cggatgcaac gctaactgcg
     2041 ctgaaagcaa tgctggaagt gaagctggaa gtcgcgccgt ggattgatct gcaaatcgtc
     2101 gccttccctc aggaagggat tttgtcgtat cccaacggtg aagcgttgct ggaagaggcg
     2161 ttacgcttag gggcagatgt agtgggggcg attccgcatt ttgaatttac ccgtgaatac
     2221 ggcgtggagt cgctgcataa aaccttcgcc ctggcgcaaa aatacgaccg tctcatcgac
     2281 gttcactgtg atgagatcga tgacgagcag tcgcgctttg tcgaaaccgt tgctgccctg
     2341 gcgcaccgtg aaggcatggg cgcgcgagtc accgccagcc acaccacggc aatgcactct
     2401 tataacgggg cgtatacctc acgtctgttc cgcttgctga aaatgtccgg tattaacttt
     2461 gtcgccaacc cgctggtcaa tattcatctg caaggacgat tcgatacgta tccaaaacgt
     2521 cgcggcatca cgcgcgttaa agagatgctg gagtccggca ttaacgtctg ctttggtcac
     2581 gatgatgtct tcgatccgtg gtatccgctg ggaacggcga atatgctgca agtgctgcat
     2641 atggggctgc atgtttgcca gctgatgggc tatgggcaga ttaacgatgg cctgaattta
     2701 atcacccacc acagcgccag gacgttgaat ttgcaggatt acagcattgc cgccggaaac
     2761 agcgccaacc tgattatcct gccggctgaa aatggatttg atgcgctgcg ccgtcaggtt
     2821 ccggtacgtt attcggtacg tggcggcaag gtgattgcca gcacacaacc ggcacaaacc
     2881 accgtatatc tggagcagcc ggaagccatc gattacaaac ggtgatcggc tgggctgtgt
     2941 gttgacaaag cgagcgtagt ttatgccgga tgcggcttga acgccttatc aggcctacgt
     3001 agagcactga actcgtaggc ctgataagcg taacgcatca ggcaattgtg tcagtggtta
     3061 ccgcgattca ttctcgccaa catccgcaca ttcctccaac gccatgtgca aaaatgcctt
     3121 cgcggcggct gtctgccagc tatttttccg ccgcaacaaa accgccgttc tctccagtag
     3181 tggcggggca agaggaatcg ctttaagccc gtcatgttgt gtggcaatcg ctgctggtaa
     3241 caatgtggaa agggaagtgc ggcgaatcag ctccagaacc gcgctaattg agttcgcctc
     3301 aatgaccacc tgtggatgca gccccgcttt ctcgcagtag tggtcaatct gctctctggt
     3361 ggcaaattcc gcgctgagca ggaccagttt ttcatcatgc aagcgactca acgccacctg
     3421 ttcacaggca gccagcggat gatgttgcgc cacgactaac gctaaacttt ctgtcagtaa
     3481 aggaattgcc tccagctccg gcaaatgcac aggcgcgaag gcaatcccaa cgtccagctc
     3541 gtcgcgacaa agcagatctt cgattttctc ctgcgacatt tcctgtagct ggagcgtgat
     3601 gccggggtag cgcgcataga aatccgccat taaggggccg ataaagtagc tcgtaaaggt
     3661 gggggtgacg gcgatacgca gcgatcctct cgtcagatcg gcaacatcat gaatcgcccg
     3721 tttacccgct cccagttcct gtaacgcccg gctggcgtac tgtcgccaga cttctcctgc
     3781 atcagtgaga tgaatcgttc gcccgctacg gtcaaacagc ggcacgccta aactctcctc
     3841 taactggcga atctgctggg aaagcgcagg ctgagagacg tgcaacgcac tggcggcacg
     3901 ggtgaagctg ccatgttcag ccacggcaag aaaataattg atatgtcgag agagcattcg
     3961 ccaacctata agtaaattca atagaactca tcataaatga gacttttacc ttatgacaat
     4021 cggcgagtag tctgcctctc attccagaga cagatagagg ttaacggtga aagagattat
     4081 tgatggattc cttaaattcc agcgcgaggc atttccgaag cgggaagcct tatttaaaca
     4141 gttggcgaca cagcaaagcc cgcgcacact ttttatctcc tgctccgaca gccgtctggt
     4201 ccctgagctg gtgacgcaac gtgagcctgg cgatcttttc gttattcgca atgcgggcaa
     4261 tatcgtccct tcctacgggc cggaacccgg tggcgtttct gcttcggtgg agtatgccgt
     4321 cgctgcgctt cgggtatctg acattgtgat ttgcggtcat tccaactgtg gcgcgatgac
     4381 cgccattgcc agctgtcagt gcatggacca tatgcctgcc gtctcccact ggctgcgtta
     4441 tgccgattca gcccgcgtcg ttaatgaggc gcgcccgcat tccgatttac cgtcaaaagc
     4501 tgcggcgatg gtacgtgaaa acgtcattgc tcagttggct aatttgcaaa ctcatccatc
     4561 ggtgcgcctg gcgctcgaag aggggcggat cgccctgcac ggctgggtct acgacattga
     4621 aagcggcagc atcgcagcgt ttgacggcgc aacccgccag tttgtgccac tggcagctaa
     4681 tcctcgcgtt tgtgccatac cgctacgcca accgaccgca gcgtaacctt atttttaaac
     4741 catcaggaga tgcaccatga ttcagtcgca aattaaccgc aatattcgtc ttgatcttgc
     4801 cgatgccatt ttgctcagca aagctaaaaa agctctctca tttgccgaga ttgccgacgg
     4861 caccgggctg gcagaagcct ttgtgaccgc ggctttgctg ggtcagcagg cgcttcctgc
     4921 cgacgccgcc cgccaggtcg gggcgaagct ggatctcgac gaagacgcca ttctactgtt
     4981 acagatgatc ccactgcgtg gctgcattga tgaccgtatt ccaaccgacc caacgatgta
     5041 tcgtttctat gaaatgttgc aggtgtacgg cacaaccctg aaagcgttgg ttcatgagaa
     5101 atttggcgat ggcattatca gcgcgattaa cttcaaactc gacgttaaga aagtggcgga
     5161 cccggaaggt ggcgaacgtg cggtcatcac cttagatggt aaatatctgc cgaccaaacc
     5221 gttctgacag ccatgcgtaa ccatcaaaag acgttcacga tgctgctggt tctggtgctg
     5281 attggtctta atatgcgacc actgctcacc tccgtcgggc cactgctacc gcaattgcgc
     5341 caggcaagcg gaatgagctt tagcgtggct gccctgttga ccgctctgcc ggtggttacc
     5401 atgggcgggc tggcgctggc cggaagctgg cttcatcagc atgtcagcga acgtcgcagc
     5461 gtcgccatca gtttgttact gattgccgtc ggtgcattga tgcgtgagct ttaccctcaa
     5521 agtgcgctgc tgcttagcag cgcactgctc ggtggggtgg ggatcggcat cattcaggcg
     5581 gtgatgcctt cggtgattaa acggcggttt cagcagcgca cgccactggt gatggggctg
     5641 tggtccgcgg ctctgatggg cggcggtggg cttggtgccg ccctgacacc ctggttagtt
     5701 caacatagcg aaacctggta tcaaacactc gcctggtggg cgctgcctgc cgttgttgcg
     5761 ctctttgcct ggtggtggca aagcacccgc gaggtcgcct cttcccacaa gacaacaacc
     5821 attccggttc gcgtggtttt cactcctcgc gcgtggacgc tgggtgttta cttcggtctg
     5881 attaacggtg gttacgccag cctgattgcc tggttacccg ccttctatat tgagattggt
     5941 gccagcgcgc agtacagcgg ttccttgctg gcattgatga cgcttgggca agccgcagga
     6001 gctttgctga tgcctgctat ggctcgccat caggatcggc gcaaactgtt aatgctggcg
     6061 ctggcgttac aactggtggg tttctgcggc tttatctggc tgccgctgca attgccggta
     6121 ttgtgggcga tggtgtgtgg tttaggtctg ggcggcgcat ttccgctctg tttgctgctg
     6181 gcgctcgatc actctgtgca accggctatt gctggcaagc tggtggcgtt tatgcaggga
     6241 atcggtttta tcatcgccgg gcttgccccg tggttttctg gcgtgctgcg tagtatcagc
     6301 ggcaattact tgatggactg ggcgtttcat gcgctgtgcg tcgtagggct gatgattata
     6361 accctgcgtt ttgcaccagc acgttttccg cagctgtggg tcaaagaggc atgatgcgac
     6421 gcttgtttct gcgctttgtt cacgccggat gcggcctaca tagatcactg aatttgtagg
     6481 cctgataagc gaagcgtatc aggcaatttt tataatttaa actgacgatt caactttgta
     6541 atctctgaaa taatattgct tatcccggtc gtttatttcg cgaataaccc gacaaggaac
     6601 gccagccgcc acgacgtttg gtggaatgtc ttttgtgacg atactacccg cgccaataac
     6661 agaattatcc ccaatggtga cgcctggatt aataaccaca tgacttccga tccagacgtt
     6721 attgccaatc gttatcggaa aagagtacat ctcaccgttt tttctcaatt catggtgtac
     6781 agggtgtccc gtaacggaaa gagtaacgtt gggtgcaatc aatacgttat caccgattgt
     6841 taccgtgtag tcatcgacaa tggttaaatt gaaatttgca taaaaattgc ggcctatatg
     6901 gatgttggaa ccgtaagaga aatagacggg gggttctacc caggcgtttt cccctaccgt
     6961 ggcaaacatt tctttaatca ggctttctct tttatcaact tctgatggat gcgagtgatt
     7021 aaactcatac attaacgttt ttctacgaag tcttttttca ggtaagcctt cgcacatatc
     7081 ggtaaatagc ttgcctgctt ttattctttc tgtcatcgac atgttcattg cgatcattcc
     7141 gttatgatat gttggtcgga taaggcgctc gcgccgcatc cgacattgat tgcttaagcg
     7201 acttcattca cctgacgacg cagtagagaa agcgggccgg ggccgctaag cgtgaacacg
     7261 gaaattaagg tgaagcccag cgccaccaga cccagcacca gataagcgcc ctggaaaccg
     7321 atgctttcat acatattacc cgctagtaca gacataaaaa tcatcgccag ttgcttaaag
     7381 aagcagaaac agaccagata aatcgtcgct gaaaaacgca cttcaaactg gctggtaata
     7441 tatttaaagc agcccaccag caggaacggt acttcaaaca tatgcagcgt tttcagaata
     7501 accacttcca gcgctgaggt ggcgaacgat gagccaataa tacgtacaga cataatagtg
     7561 ccagccagca gcagggcatt tttcccaccg atgcgattaa tgatcagtgg cgcaaagaac
     7621 ataattgagg cgttaagtaa ttcgcccatt gtcgttacgt agccaaatac ccgcgtaccc
     7681 tgttcaccgg tggcaaagaa agaagtaaag aaattagcaa actgttggtc aaaaacatcg
     7741 taggtgcagg aaacgccaat aacatacagt gacaaaaacc acagttttgg ctgtctgaac
     7801 agttccagcg ccagtttaag gctaaatgcc gaatggttgg cacctaccgc attggcaacc
     7861 gtggcggaag agggcgcatc cgttttggcg aaaaagagta aaatggcgag gatgagtgca
     7921 cagccagaac ccagccagaa aacgaactga ttattgatgg tgaacatgat gccgacaatc
     7981 gaggcacaca gcgcccagcc aacacagcca aacatccgcg cgcgaccaaa ttcgaaatta
     8041 ctgcgacggc tgactttctc gataaatgcc tctactgcgg gcgcaccggc gttaaaacaa
     8101 aagccaagat aaataccacc aacaatcgat cctactaaaa tgttgtattg taacagtggc
     8161 ccgaagataa aaataaagaa cggcgcaaac atcactaaca tgccggtaat aatccacagc
     8221 aggtatttgc gcagcccgag tttgtcagaa agcagaccaa acagcggttg gaataatagc
     8281 gagaacagag aaatagcagc aaaaataata cccgtatcac ttttgctgat atggttgatg
     8341 tcatgtagcc aaatcgggaa aaacgggaag taggctccca tgataaaaaa gtaaaagaaa
     8401 aagaataaac cgaacatcca aaagtttgtg ttttttaaat agtacataat ggatttcctt
     8461 acgcgaaata cgggcagaca tggcctgccc ggttattatt atttttgaca ccagaccaac
     8521 tggtaatggt agcgaccggc gctcagctgg aattccgccg atactgacgg gctccaggag
     8581 tcgtcgccac caatccccat atggaaaccg tcgatattca gccatgttcc ttcttccgcg
     8641 tgcagcagat ggcgatggct ggtttccatc agttgttgtt ggctgtagcg gctgatgttg
     8701 aactggaagt cgccgcgcca ctggtgtggg ccataattca attcgcgcgt cccgcagcgc
     8761 agaccgtttt cgctcgggaa gacgtacggg gtatacatgt ctgacaatgg cagatcccag
     8821 cggtcaaaac aggcggcagt aaggcggtcg ggatagtttt cttgcggccc taatccgagc
     8881 cagtttaccc gctctgctac ctgcgccagc tggcaggtca ggccaatccg tgccggatgc
     8941 ggtgtatcgc tcgctacttc aacatcaacg gtaatcgcca tttgaccact accatcaatc
     9001 cggtaggttt tccggctaat aaataaggtt tttccctgat gctgccatgc gtggacagtg
     9061 gtaatcagca ccgcgtcggc aagtgtatct gccgtgcact gcaacaacgc cgcttctgcc
     9121 tggtaatggc ccgcagcctt ccagcgttcg acccaggcgt tagggtcaat gcgggtcgct
     9181 tcacttacgc caatgtcgtt atccagcggt gcgcgggtga actgatcgcg cagcggcgtc
     9241 agcagttgtt ttttatcgcc aatccacatc tgtgaaagaa agcctgactg gcggttaaat
     9301 tgccaacgct tattatccag ctcgatgcaa aaatccgttt cgctggtggt cagttgcggg
     9361 atggcgtggg gcgcggaggg gagtgtcacg ctgaggtttt ccgccagacg ccactgctgc
     9421 caggcgctga tgtgtccggc ttctgaccat gcggtcgcgt tcggttgcac tacgtgtacc
     9481 gttagccaga gttgcccggt gctctccagt cgcggtagtt caggcaattc aattacctgt
     9541 ttaccttgcg gagcgacatc cagaggcact tcaccgcttg ccagcggctt accatccagc
     9601 gccaccgtcc agtgcaggag ctcgttatcg ctatgacgga acaggtattc gctggtcact
     9661 tcgatggttc gcccggataa acggaactgg aaaaactgct gctggtgttt tgcttccgtc
     9721 agcgccggat gcggcgtgcg gtcggcaaag accagaccgt tcatgcagaa ctggcgatca
     9781 ttcggcgtat cgccaaaatc accgccgtaa gccgaccacg gattgccatt ttcatcatat
     9841 ttaatcagcg actgatccac caagtcccag acgaagccgc cctgtaaacg ggggtactga
     9901 cgaaacgcct gccagtattt agcgaagccg ccaagactgt tacccatcgc gtgggcgtat
     9961 tcgcaaagga tcagtgggcg catttctcca ggcagcgaaa gccatttttt gatggaccat
    10021 ttcggcacag ccgggaaggg ctggtcttca tccacgcgcg cgtacatcgg gcaaataata
    10081 tcggttgcgg aggtgtcggc tccgccgcct tcatactgca ccgggcggga tggatcgaca
    10141 gatttaatcc agcgatagag tgcgtcgtga ttagcgccgt ggcctgactc attccccagc
    10201 gaccagatga tcacactcgg gtgattacga tcgcgctgca ccattcgtgt tactcgttcg
    10261 ctcatggccg gtaaccagcg cggatcatcg gtcagacgat tcattggcac catgccgtgg
    10321 gtttcaatat tggcttcatc caccacatac aggccgtagc ggtcgcacag ggtgtaccac
    10381 agcggatggt tcggataatg cgaacagcgc acggcgttaa agttgttctg cttcattagc
    10441 aggatatcct gcaccatcgt ctgctcatcc atgacctgac catgcagagg atgatgctcg
    10501 tgacggttaa cgccgcgaat cagcaacggc ttgccgttca gcagcagcag accattttca
    10561 atccgcacct cgcggaaacc gacatcgcag gcttctgctt caatcagcgt gccgtcggcg
    10621 gtgtgcagtt caaccaccgc acgatagata ttcgggattt cggcgctcca cagtttcggg
    10681 ttttcgacgt tcagacctag tgtgacgcga tcggcataac cgccacgctc atcgataatt
    10741 tcaccgccga aaggcgcggt gccgctggcg acctgcgttt caccctgcca caaagaaacc
    10801 gtcacccgca gctcatcgcg cagctcgccg tacatctgaa cttctgcctc cagtaccgcg
    10861 cggctaaaat catcattaaa gagagtggca acatggaaat cgctgatttg tgtggtcggt
    10921 ttgtgcagca acgagacgtc acggaaaatg ccgctcatcc gccacatatc ctgatcttcc
    10981 agataactgc cgtcactcca gcgcagcacc atcaccgcga ggcggttttc tccggcgcgt
    11041 aaaaatgcgc tcaggtcaaa ttcagacagc aaacgactgt cctggccgta accgacccag
    11101 cgcccgttgc accacagatg aaacgccgag ttaacgccat caaaaataat tcgcgtctgg
    11161 ccttcctgta gccagctttc atcaacatta aatgtgagcg agtaacaacc cgtcggattc
    11221 tccgtgggaa caaatggcgg attgaccgta atgggatatg tcacgttggt gtagatgggc
    11281 gcgtcgtaac cgtgcatctg ccagtttgag gggacgacga cagtatcggc gtcaggaaga
    11341 tcgcactcca gccagctttc cggaaccgct tctggtgccg gaaaccagac aaattgccac
    11401 tcaccattca ggctgcgcaa ctgctgggaa gggcgattgg tgcgggcctc ttcgctatta
    11461 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
    11521 tcccagtcac gacgttgtaa tacgacggcc agtgaatctg taatcatagt catagctgta
    11581 tcctgtgtga aattgttatc cgctcacaat ttcacacaac atacgagccg gacgcataga
    11641 gtgtaaagcg tggggtgcct aatgagtgag ctgactcaca ttaattgcgt tgcgctcact
    11701 gcccgctttc cagtcgggaa acttgtcgtg ccagctgcat taatgaatcg gccaacgcgc
    11761 ggggagaagc ggtttgcgtc ttgggcgcca gggtggtttt tcttttcacc agtgagacgg
    11821 gcaacagctg attgcccttc accgcctggc cctgagagag ttgcagcaag cggtccacgc
    11881 tggtttgccc cagcaggcga aaatcctgtt tgatggtggt taacggcggg atataacacg
    11941 agctgtcttc ggtatcgtcg tatcccacta ccgagatatc cgcaccaacg cgcaacccgg
    12001 actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc agcatcgcag
    12061 tgggaacgat gccctcattt agcatttgca tggtttgttg aaaaccggac atggcactcc
    12121 agtcgccctc ccgttccgct atcggctgaa tttgattgcg agtgagatat ttatgccagc
    12181 ccgccagacg cagacgcgcc gagacagaac ttaatgggcc cgctaacagc gcgatttgct
    12241 ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa
    12301 taatactgtt gatgggagtc tggtcagaga catcaagaaa taacgccgga acattagcgc
    12361 aggcagcttc cacagcaatg gcatcctggt catccagcgg atagttaatg atcagcccac
    12421 tgacgcgttg cgcgagaaga ttgtgcactg ccgctttaca ggcttcgacg ccgcttcgtt
    12481 ctaccatcga caccaccacg ctggcaccca gttgatcggc gcgagattta atcgccgcga
    12541 caatttgcga cggcgcgtgc agggccagac tggaggtggc aacgccaatc agcaacgact
    12601 gtttccccgc cagttgttgt gccacgcggt tgggaatgta attcagctcc gccatcgccg
    12661 cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc ctgattcacc acgcgggaaa
    12721 cggtctgata agagacaccg gcatactctg cgacatcgta tagcgttact ggtttcacat
    12781 tcaccaccct gaattgactc tcttccgggc gctatcatgc cataccgcga aaggttttgc
    12841 gccattcggc ggggctgacg ccgaagtgag atttaaaatg ctgacgccag gtcacaggtg
    12901 agagaaaacc cacctcagcc gctatccgct caacgggcaa ctgactggat cccaacagtt
    12961 cctggctacg gcgtaagcgt tcagtaatga gccattcggc gatactcgaa ccggtcgctt
    13021 tcataaaatg gcgagttaat gtgcggcgac tcatcattac tcgctgcgcc agcgagtcga
    13081 gatcatgctg ttgcgcaatg ttttgccgca ggtaatccag caggaggttt atgcgttcat
    13141 catgggtatt tttcggcacc ggttgctcaa taaactgcgc ctgaccgccg gtgcgatatg
    13201 gcggtacgac catccgtcgg gcaatatggt tagcatagtc gctgccaaaa tgttgccgaa
    13261 caatatacaa acagcaatcc agcgccgcgg cagtaccggc agaggtaata atgccgtcat
    13321 cgtctacgta caacgcgtta atatccagat gtgtttgtgg aaaacgtgcc tgaaattcac
    13381 gctcgaactc ccagtgcgtg gcggcacgct tatttttcag taaacctgca tagccgagca
    13441 caaacgtgcc caggcaaagc ccgacaatct gtgcgccgtt atcccgtgcg gtcgttaagg
    13501 cttccagcag tttttgtggc ggtttttgtg taatggttcc ccagtaagga attatcacaa
    13561 tatctgcctg gatgactgcg gtatagtcat gggtggcggt tacggaaaac ccatcggcag
    13621 agtccacgtt tcccggcttt tcagcgcaaa taatcacgtg aaagcgtttt ttctccgcca
    13681 tcttttcact aaacacgata aacggtacgg aaaggtgaaa aggactaaaa ccggcaacgg
    13741 cgataatggc aacagttagc gggcgcataa cactctttat ctccgaatta gaatgtcaac
    13801 gtttcaccat cttctggtac gttcacaaat tcgataattt gattatctct ggcgtattcc
    13861 ttgagcgcat cacgcgtcag caaacagtgg tttacggcct ccatatgtgt cgcgataatt
    13921 tgcgcttctg ggagggtaaa atgtgttttg agaatatctt ctgctcccat aattatcgcg
    13981 ccatgcccca gaacatgcgc aaatccggca ttcaacacaa ccacttctgg ttggaaagtt
    14041 agcattgtag cctcgacgtc gtcacgccag agggtatcgc cagccagata gagcgttttc
    14101 tctgtcgggt gctggaaaac aacgccgcaa gctgcgccta aacgcttcgc catcgccggg
    14161 tcggcatagg tgcgatcgga gccgtgctgc cctgtcgttt tatgcagagt aatatcgcca
    14221 aatttagtct cgtcggtaag tacggtaaga tgcgtaaatc cttgtttatt cagtgattca
    14281 gcatcttgct cattttgcac ataaatcagt ttatctttag ggacgacgcg ggaggctgtt
    14341 tcgtcccagt gatcttcatg caaatgggtc acgattaggg cgtcaacgtt gagtaacgtt
    14401 gttaaatcgc acggtaattc taccgttgga ttgcggattt ctgcgcgtgc cgttccggca
    14461 aaacccggat aacttccttt aggcgcgagt aacgggtcga taagaaatgt tttgccagcg
    14521 tattggataa tttgggtggc attacgaatt tgcgtaattt tcatgatttg ctccacatca
    14581 cagtattgat gtcattagcg tagcgtgcag cccgtactgt gcatgtgggc aaatgggcga
    14641 ataacgatga gaatgggcca aattacacat tggcgtaact agcgtaaatg cacgccgctt
    14701 cgccgtccgg ccaccagaat agcctgcgat tcaacccctt cttcgatctg ttttgctacc
    14761 cgttgtagtg ccggaagatg cttttccgct gcctgttcga tggtcattgc gctcgccata
    14821 tacaccagat tcagacagcc aatcacccgt tgttcactgc gcagcggtac ggcgatagag
    14881 gcgatcttct cctcctgatc ccagccgcgg tagttctgtc cgtaaccctc tttgcgcgcg
    14941 cgcgccagaa tggcttccag ctttaacggt tctcgtgcca gttgatagtc atcaccgggg
    15001 cgggaggcta acatctcgat taattccttg cgttcttgtt ccgggcaaaa ggccagccag
    15061 gtcaggcccg aggcggtttt cagaagcggt aaacgccgcc cgaccattgc ccggtgaaag
    15121 gataagcggc tgaaacggtg agtggtttcg cgtaccacca ttgcatcaac atccagcgtg
    15181 gacacatctg tcggccatac cacttcgcgc aacagatcgc ccagcagtgg ggccgccagt
    15241 gcagaaatcc actgttcgtc acgaaatcct tcgcttaatt gccgcacttt gatggtcaga
    15301 cgaaaactat catcggaggg actacgccgg acatatccct cttcctgcag cgtctccagc
    15361 agtcgccgca cagtggtgcg atgcaggccg ctgagttccg ccagcagccc gacgctggca
    15421 ccgccgtcaa gtttatttaa catatttaat aacattagac cgcgggttaa gccgcgcacg
    15481 gttttgtatt ccgtctgctc attgttctgc atattaattg acatttctat agttaaaaca
    15541 acgttgtgca cctggtgcac attcgggcat gttttgattg tagccgaaaa caccctttct
    15601 atactgagcg cacaataaaa aatcatttac atatttttaa caaaataagt tgcgctgtac
    15661 tgtgcgcgca acggcatttt gtccgagtcg tgaggtactg aaatggcaat acaacaccct
    15721 gacatccagc ctgctgttaa ccatagcgtt caggtggcga tcgctggtgc cggtccggtc
    15781 gggctgatga tggcgaacta tctcggccag atgggcattg acgtgctggt ggtggagaaa
    15841 ctcgataagt tgatcgacta cccgcgtgcg attggtattg atgacgaggc gctgcgcacc
    15901 atgcagtcgg tcggcctggt cgataatgtt ctgccgcata ctacgccgtg gcacgcgatg
    15961 cgttttctca ccccgaaggg ccgctgtttt gctgatattc agccaatgac cgatgaattt
    16021 ggctggccgc gccgtaacgc ctttattcag ccgcaggtcg atgcggtgat gctggaaggg
    16081 gtgtcgcgtt ttccgaatgt gcgctgcttg ttttcccgcg agctggaggc cttcagtcag
    16141 caagatgacg aagtgacctt gcacctgaaa acggcagaag ggctgcggga aatagtcaaa
    16201 gcccagtggc tggtggcctg tgatggtggg gcaagttttg tccgtcgcac cctgaatgtg
    16261 ccgtttgaag gtaaaactgc gccaaatcag tggattgtgg tagatatcgc caacgatccg
    16321 ttaagtacgc cgcatatcta tttgtgttgc gatccggtgc gcccgtatgt ttctgccgcg
    16381 ctacctcatg cggtacgtcg ctttgaattt atggtgatgc cgggagaaac cgaagaacag
    16441 ctgcgtgagc cgcaaaatat gcgcaagctg ttaagcaaag tgctgcctaa tccggacaat
    16501 gttgaattga ttcgccagcg tgtctacacc cacaacgcgc gactggcgca acgtttccgt
    16561 attgatcgcg tactgctggc gggcgatgcc gcgcacatca tgccggtatg gcaggggcag
    16621 ggctataaca gtggtatgcg cgacgccttt aacctcgcat ggaaactggc gttggttatc
    16681 caggggaaag cccgcgatgc gctgctcgat acctatcaac aagaacgtcg cgatcacgcc
    16741 aaagcgatga ttgacctgtc cgtgacggcg ggcaacgtgc tggctccgcc gaaacgctgg
    16801 cagggtacgt tacgtgacgg cgtttcctgg ctgctgaatt atctgccgcc agtaaaacgc
    16861 tacttcctcg aaatgcgctt caagccgatg ccgcaatatt acggcggtgc gctggtgcgt
    16921 gagggcgaag cgaagcactc tccggtcggc aagatgttta ttcagccgaa agtcacgctg
    16981 gaaaacggcg acgtgacgct gctcgataac gcgatcggcg cgaacttcgc ggtaattggc
    17041 tggggatgca atccactgtg ggggatgagc gacgagcaaa tccagcagtg gcgcgcgttg
    17101 agcacacgct tcattcaggt ggtgccggaa gtgcaaattc ataccgcaca ggataaccac
    17161 gacggcgtac tacgcgtggg ggatacgcaa ggtcgcctgc gtagctggtt cgcgcaacac
    17221 aatgcttcgc tggtggtgat gcgcccggat cgctttgttg ccgccaccgc cattccgcaa
    17281 acactgggca agaccctgaa taaactggcg tcggtgatga cgctgacccg ccctgatgcc
    17341 gacgtttctg tcgaaaaggt agcctgatat gcacgcttat cttcattgtc tttcccactc
    17401 gccgctggtg gggtatgtcg acccggcaca agaggtactc gatgaggtca atggcgtgat
    17461 tgccagcgcc cgcgagcgta ttgcggcttt ctcccctgaa ctggtggtgc tgtttgcgcc
    17521 agatcactac aacggctttt tctatgacgt gatgccaccg ttctgtttag gcgttggagc
    17581 gacggcaatt ggtgatttcg gcagtgcggc aggagagctg cccgtgcctg tggagctggc
    17641 ggaggcctgt gcgcatgccg tcatgaagag cgggatcgat cttgccgttt cttactgtat
    17701 gcaggtggac cacgggttcg cccagccgct ggagttcctg ctcggtgggc tggataaggt
    17761 gccagttctg cctgtgttca tcaacggtgt cgccacgccg ctgcccggtt tccagcgtac
    17821 ccgcatgttg ggtgaagcca ttggacgttt caccagcact ctcaataaac gcgtgctgtt
    17881 cctaggttcc ggtgggcttt cccatcagcc gccggtgccc gaactggcga aagccgatgc
    17941 ccatatgcgc gaccgcctgt tggggagcgg gaaagattta cccgccagtg agcgcgaatt
    18001 gcgtcagcaa cgggtgatta gcgccgctga gaagtttgtt gaggatcaga gaactctgca
    18061 tccgctcaac ccgatttggg ataaccagtt catgactttg ctggagcagg gacgcataca
    18121 ggaactggat gccgtcagta acgaagagct ttccgccatt gccggaaagt cgacacatga
    18181 aatcaaaacc tgggtcgccg cttttgccgc tatttctgca tttggcaact ggcgtagcga
    18241 agggcgttat taccgcccaa tcccggagtg gattgccgga tttggctcgt taagcgccag
    18301 aacagagaac tgaatatgca ggagaagatg atgagttatc agccacaaac cgaagccgcc
    18361 accagccgtt ttctgaatgt agaagaagcg ggtaaaacgc tgcgcatcca ttttaatgac
    18421 tgcggacaag gcgacgaaac cgttgtcctg ctgcatggtt ccggcccggg tgctactggc
    18481 tgggcgaact tcagccgcaa tatcgatccg ctggtagagg cgggctatcg ggtgatcctg
    18541 ctggattgtc cgggttgggg caagagcgat tcgatcgtta atagtggttc gcgatcggat
    18601 cttaatgcac gaatcctgaa aagcgtggtg gatcaactgg atatcgccaa aatccacctg
    18661 ctgggcaact cgatgggtgg ccatagttct gtggcgttca cccttaactg gccggagcgc
    18721 gtcggcaaac tggtgctgat gggcggcggt acgggcggca tgagtttgtt tacgccgatg
    18781 ccaaccgaag gtattaagcg actgaatcag ctttatcgtc agccgactat cgaaaacctg
    18841 aagctgatga tggatatctt cgtttttgat accagcgatt tgaccgacgc cctgtttgaa
    18901 gcgcgcctga ataatatgct gtcgcgccgc gatcacctgg aaaacttcgt taagagcctg
    18961 gaagctaatc cgaaacagtt cccggatttt ggcccacgtc tggcggaaat caaagcgcaa
    19021 accctgattg tctgggggcg caacgaccgc tttgtgccga tggatgcggg tctgcgtctg
    19081 ctgtccggca ttgccggttc tgaactgcat atcttccgcg actgtgggca ttgggcgcag
    19141 tgggaacatg ccgacgcttt caatcaactg gtgctgaatt tcctcgcacg tgcttaagga
    19201 acggtcatga cgaagcatac tcttgagcaa ctggcggcgg atttacgccg cgccgcagag
    19261 cagggcgaag cgattgcgcc gctgcgcgat ctgattggta tcgataacgc tgaagcggcc
    19321 tacgccattc agcacataaa tgtgcaatat gacgttgcgc aggggcgtcg cgtggtgggg
    19381 cgtaaagtgg gcctgacaca tccgaaagtg cagcaacaac tgggcgttga tcaaccggat
    19441 ttcgggacat tatttgccga catgtgttat ggcgataacg aaatcattcc tttttcccgt
    19501 gttctgcaac cccgcattga agcggagatc gcactggtgt tgaaccgcga tttacccgca
    19561 actgatatca ccttcgacga attgtataac gccattgaat gggtacttcc ggcgctggag
    19621 gtggtgggta gccgcattcg cgactggtcg attcagtttg tcgataccgt ggcagataac
    19681 gcttcctgcg gggtgtatgt catcggcggt ccggcgcaac gtccggcggg gttagacctg
    19741 aaaaactgcg ccatgaagat gacgcgtaat aacgaagagg tttccagcgg gcgcggcagc
    19801 gaatgcctgg gacatccgct taatgccgcc gtctggctgg cacgcaaaat ggccagtctg
    19861 ggtgaaccgc tgcgcgccgg agatatcatt cttaccgggg cattaggtcc gatggtggca
    19921 gtgaatgcgg gcgatcgttt tgaagcccat attgaaggca taggttcagt tgctgcggca
    19981 ttttcaagcg cagccccaaa aggaagtctg tcatgagtaa gcgtaaagtc gccattatcg
    20041 gttctggcaa cattggcacc gatctgatga ttaaaatttt gcgtcacgat cagcatctgg
    20101 agatggcggt gatggtcggc attgatcctc agtccgacgg tctggcgcgt gccagacgta
    20161 tgggcgtcgc caccacccat gaaggggtga tcgggctgat gaacatgcct gaatttgctg
    20221 atatcgacat tgtatttgat gcgaccagcg ccggtgctca tgtgaaaaac gatgcggctt
    20281 tgcgcgaagc gaaaccggat attcgcttaa ttgacctgac gcctgctgcc atcggccctt
    20341 actgcgtgcc ggtggttaac ctcgaggaga acgtcgatca actgaacgtc aacatggtca
    20401 cctgcggcgg ccaggccacc attccaatgg tggcggcagt ttcacgcgtg gtgcgtgttc
    20461 attacgccga aattatcgct tctatcgcca gtaaatctgc cggacctggc acgcgtgcca
    20521 atatcgatga atttacggaa accacttcgc gagccattga agtggtgggc ggcgcggcaa
    20581 aagggaaggc gattatcgtg cttaacccag cagagccacc gttgatgatg cgtgacacgg
    20641 tgtatgtatt gagcgacgaa gcttcacaag atgatatcga agcctcaatc aatgaaatgg
    20701 ctgaggcggt gcaggcttac gtaccgggat atcgcctgaa acagcgcgtg cagtttgaag
    20761 ttatcccgca ggataaaccg gttaatttgc cgggcgtagg gcagttctcc gggctgaaaa
    20821 cggcggtctg gctggaagtc gaaggcgcag cgcattatct gcctgcctat gcgggcaacc
    20881 tcgacattat gacttccagt gcgctggcga cagcagaaaa aatggcccag tcactggcgc
    20941 gcaaggcagg agaagcggca tgaacggtaa aaaactttat atctcggacg tcacgttgcg
    21001 tgacggtatg cacgccattc gtcatcagta ttcgctggaa aacgttcgcc agattgccaa
    21061 agcactggac gatgcccgcg tggattcgat tgaagtggcc cacggcgacg gtttgcaggg
    21121 gtccagcttt aactatggtt tcggcgcaca tagcgacctt gaatggattg aagcagcggc
    21181 ggatgtggtg aagcacgcca aaatcgcgac gttgttgctg ccaggaatcg gcactattca
    21241 cgatctgaaa aatgcctggc aggctggcgc gcgggtggtt cgtgtggcta cgcactgtac
    21301 cgaagctgat gtttccgccc agcatattca gtatgcccgc gagctcggaa tggacaccgt
    21361 tggttttctg atgatgagcc atatgaccac gccggagaat ctcgcaaagc aggcaaagct
    21421 gatggaaggc tacggtgcga cctgtattta tgtggtggat tctggcggtg cgatgaacat
    21481 gagcgatatc cgtgaccgtt tccgcgccct gaaagcagtg ctgaaaccag aaacgcaaac
    21541 cggcatgcac gctcaccata acctgagtct tggcgtggcg aactctatcg cggcggtgga
    21601 agagggctgc gaccgaatcg acgccagcct cgcgggaatg ggagcgggcg caggtaacgc
    21661 gccgctggaa gtatttattg ccgccgtgga taaactgggt tggcagcatg gggccgatct
    21721 ctatgcgtta atggatgctg ctgacgacct ggtgcgtccg ttgcaggatc gaccggtacg
    21781 agtcgatcgc gaaacgctgg cgctgggata cgctggtgtt tactccagct tcctgcgtca
    21841 ctgtgaaacg gcggcggcgc gttatggctt aagcgcggtg gatattctcg ttgagctggg
    21901 caaacgccgg atggttggcg gccaggagga tatgatcgtc gacgtggcgc tggatctgcg
    21961 caacaacaaa taatattgac tgccgagaac gtgcattttg tatgcccatc ccagcacgac
    22021 tgccggatgc ggcgtgaacg ccttatccgg tctacatttc gcccgtaggc agtcattaaa
    22081 tagttctgat tacgcggctg gcaatgtatc agtcgcgatt cacctgcaca cgcaatgagg
    22141 ttcatcttat gtcgactcgt accccttcat catcttcatc ccgcctgatg ctgaccatcg
    22201 ggctttgttt tttggtcgct ctgatggaag ggctggatct tcaggcggct ggcattgcgg
    22261 cgggtggcat cgcccaggct ttcgcactcg ataaaatgca aatgggctgg atattcagtg
    22321 ccggaatact cggtttacta cccggcgcgt tggttggcgg aatgctggcg gaccgttatg
    22381 gtcgcaagcg cattttgatt ggctcagttg cgctgtttgg tttgttctcg ctggcaacgg
    22441 cgattgcctg ggatttcccc tcactggtct ttgcgcggct gatgaccggt gtcgggctgg
    22501 gggcggcgtt gccgaatctt atcgccctga cgtctgaagc cgcgggtcca cgttttcgtg
    22561 ggacggcagt gagcctgatg tattgcggtg ttcccattgg cgcggcgctg gcggcgacac
    22621 tgggtttcgc gggggcaaac ttagcatggc aaacggtgtt ttgggtaggt ggtgtggtgc
    22681 cgttgattct ggtgccgcta ttaatgcgct ggctgccgga gtcggcggtt ttcgctggcg
    22741 aaaaacaggc tgcgccacca ctgcgtgcgt tatttgcgcc agaaacggca accgcgacgc
    22801 tgctgctgtg gctgtgttat ttcttcactc tgctggtggt ttacatgttg atcaactggc
    22861 taccgctgct tttggtggag caaggattcc agccatcgca ggcggcaggg gtgatgtttg
    22921 cactgcaaat gggtgcggcg agcgggacgt taatgttggg cgcattgatg gataagctgc
    22981 gtccagtaac catgtcgcta ctgatttata gcggcatgtt agcttcgctg ctggcgcttg
    23041 gaacggtgtc gtcatttaac ggtatgttgc tggcgggatt tgtcgcgggg ttgtttgcga
    23101 caggtgggca aagcgttttg tatgccctgg caccgttgtt ttacagttcg cagatccgcg
    23161 caacaggtgt gggaacagcc gtggcggtag ggcgtctggg ggctatgagc ggtccgttac
    23221 tggccgggaa aatgctggca ttaggcactg gcacggtcgg cgtaatggcc gcttctgcac
    23281 cgggtattct tgttgctggg ttggcggtgt ttattttgat gagtcggaga ttaagaatgc
    23341 agccgtgtgc cgatgcctga cgcgccttat taagcagggg cgaaagggac ttgcccccaa
    23401 tacacccacg gcgtttacag gtatactcgc taaaaattat tcagcgggtt tggaaacaaa
    23461 gatggcaaaa cttaccttac aagagcagtt gctcaaagca ggattagtca ccagcaaaaa
    23521 agcggcgaag gtcgagagaa cggcgaaaaa atcgcgcgtt caggcgcgtg aagctcgggc
    23581 ggcggtagaa gaaaataaaa aggcacagct tgagcgtgat aaacagctta gcgaacagca
    23641 aaaacaagcg gcgttggcga aagaatataa agctcaggtg aagcagctta ttgaaatgaa
    23701 ccggatcacc attgccaatg gcgatattgg ttttaacttc actgatggca atctgattaa
    23761 gaagattttt gtcgataagc tcacacaggc gcagttgatt aatggtcgtc tggcgattgc
    23821 ccgcttgttg gtcgataaca atagcgaagg tgaatacgcc attattcccg ccagcgttgc
    23881 cgataaaatt gctcagcgtg atgccagcag tattgtgttg cacagcgcgc tcagtgccga
    23941 agagcaggat gaagacgacc cgtatgccga cttcaaagtg cctgatgatt tgatgtggta
    24001 aacatagata agtcgtgcgc agatgcctga tgcgacgcta tgcgcgtctt atcatgccta
    24061 cggcctgcgg gcgaaatgta ggcggataag gcgttcacgc cgcatccggc agtcgtgcac
    24121 tattatcaac gcatattcag tttattggcg tggtaggcaa tatgctcgcc aataaaactg
    24181 gagacaaaat aatagctgtg gtcgtaaccc tcgtgataac ggattaacgt tttgatattc
    24241 atctcctggc agatcttttc aagattttga gtccgcagct gttccgcgta aaaatcatca
    24301 ctcaacccct gatcaaccat gatttccgca acgcgttgac cttgtgaaat aagactcacc
    24361 ggatcgtaat ccaaccaggc ctctttattt tcaccaagat atgcggcaaa ggcttgctgt
    24421 ccccacggta cttgcgatgg ggagacaatg ggcgaaaacg ccgagacgct gacatattca
    24481 tctgggttac gtaacgccag caccagcgcg cccagtccgc ccatagaatg accagagata
    24541 gactttttgg ccgttgccgg aaaatgattc atcactaaat ccggcagctc gttgcggata
    24601 tagtcataca ttttgtaatg ttcattccac ggcgcttgcg tcgcgttcag gtaaaacccg
    24661 gcaccttgcc cgagatcgta acggtcggca tctgcgacat gactgcctcg cggactggtg
    24721 tccggcgcaa caacaataat gttgtgctca gccgcgtaac gctgcacccc cgatttagta
    24781 atgaaattct gctcattgca ggtcaggccc gaaagccagt acagcaccgg taatttttca
    24841 cctgcagctt ttggtgggag atagacgccg acattcattt cacatttcag tgatgcggaa
    24901 taatgccgat acacattttg ccagccgcca aagctggcat gtttttcaat gagttccatc
    24961 agtcattatc tcacggttaa tcccccggac ggctgcccgg agagagtatt aagaaccccg
    25021 tctgaagtgg acggggtaaa ccagcgggaa atcagtaacg aattacggtt cgaatggatt
    25081 tgccttcatg catcaggtcg aaggcgtcat taatttcatc caggctcatg gtatgcgtga
    25141 caaacggttc cagatcgata tcacctttca tcgcatcttc aaccataccc ggtaactggg
    25201 aacgaccttt cacgccgcca aacgcggaac ctttccatac gcgaccagtg accaactgga
    25261 atggacgggt ggagatttcc tgaccggaac ccgcgacccc gatgatcacc gactgacccc
    25321 agccgcggtg cgcactttcc agcgccgcac gcatcacgtt gacattaccg atgcattcaa
    25381 aggtatggtc gataccccat ttgttgatat ccagcaggac atctttaatc ggtttgtcgt
    25441 agtcattcgg gttaatgcag tcggtggcac cgaagcgacg cgccagatcg aatttcttcg
    25501 ggttggtatc gatagcgata atccgtcccg ctttcgcctg acgcgcgccc tgaaccactg
    25561 ccagaccaat cgcgccaaga ccaaacacag caacagaatc ccctggctgg actttagctg
    25621 tgttgtgtac cgcgccaata ccggtggtca cgccacagcc cagcaggcag acgtgttcat
    25681 ggtttgcttc tggattaatt tttgccagag acacttccgc gactacggtg tattcactga
    25741 atgtagaaca ccccatgtag tgataaagcg gctgcccatt gtaagaaaaa cgggtggtgc
    25801 cgtctggcat caggccttta ccctgggttt cgcgaaccgc aacacagagg ttagttttgc
    25861 cagaacgaca gaactcacac tcgccgcact ctgcggtgta aagcgggatc acatggtcgc
    25921 ctggtttgac gctggttacg ccttcaccga cttcaaccac aacgccagcc ccttcgtgac
    25981 cgagaaccac cgggaataca ccttccgggt catcgccgga gagggtaaat gcgtcggtat
    26041 ggcaaacgcc ggtatgggtg actttaatca gcacttcacc ttttttcggt ggtgcaacgt
    26101 caatttcaac gatttccagc ggtttaccgg gagcaaatgc aacggcagca cgtgatttca
    26161 tctctcgctc ttcctcaata tggtaataga ttcagctatt taagataggc acgaaccagt
    26221 tcaatagtgt cgtcaacgga ttggctgact tcgcggctgt agcagtcatt tcggtcaaac
    26281 gtttcccgga tatggctttc aagcacttct gccatcagcc cattagccgc gccccgaacg
    26341 gcagcgatct gttggagtat ggcacggcat tcggcatcac cctccagcga ccgttccaga
    26401 gcatcaatct gccctcgaat acgacgaact cgagtaagga cctttttctt ctcttccgga
    26461 gtactgggca tttcgcacct catcatctgc acgcaatata ctataggggg gtatgctata
    26521 tgtcaatgca taccccccta tagtatataa caatggagaa taattgttgt ccgaagccta
    26581 accaggtgaa cggtccctca agacagatgg ggaccgttta tgtgctattt ttatgccgca
    26641 tccgctaaaa caaacatccc gtaaggatgg atttcgaggt aatagtgctc accaatatcg
    26701 ggctgtaaac gggtggcgtt tacttgtaat aaaatctcct gtccatgcca ctctacggtc
    26761 acttcgtact gcggccccat gtaggcgaca tggcgaataa cacaacgctg actctcttcg
    26821 ccgtgattgc ttaaggtgat ggcttccggg cgaacgccga cggtgccgct accttgcacc
    26881 gggaaatgcg ccgcgcgcgg caggcgataa ccgtagatat cgacatactc ttcgctaaag
    26941 ttcgccggga acaggttggc gtcgcccata aaacttgcca taaagcggga ggctggctga
    27001 cgatagagat cctgcggtga gccaatctgc atgatgtgac ctttattcat caccagcaca
    27061 gtgtcggaaa ccgcgaaggc ctcactttga tcgtgagtca cgtagagcga cgtgatatta
    27121 aactgctttt gcagctcgcg gatcttatcg cgcatgctgc ggcgcaggtt ggcatcgagg
    27181 ttacttaacg gctcatcaaa cagcagcacc tttggtttga ggatcaacgc gcgggccagc
    27241 gccacgcgct gttgctgacc accggaaatc tggtcgacat agcggtcctc gaacccttcc
    27301 agatccacca ttgccagcgc ctctttcacc cgctgcttta cttcgctacg cgacacgccg
    27361 agcatcttta acccgtagcc gacgttttcg cccagcgaca tatgcgggaa cagagcgtat
    27421 gactgaaaca ccatgcagat atcgcgctgc tgaatggaac gatgcgtgac atcttcgcca
    27481 tcaataaaga tttgcccttc actcggtttt tccagcccgg caaccagacg caaaatggtg
    27541 gttttgccgc agccggaagg gccaagcagc gtcaccattt gcccttgtgg gatggtgaga
    27601 ttgatattgt cgataaccgt attactgccg aatcgtttag tgacgttgcg cagttccaca
    27661 aaatttttct gactcatagt gcgctccatt acgcctggtt tttggcttta gaacgggaga
    27721 tacgtgcttc tccgatcagc cagtcaaaga ggaaaataat cgccagcatc accacgatca
    27781 gaatcgaacc ataggcgatg gctacgccat attcgccgtc ttcaacgcgg ttaagaatgt
    27841 aggcggttgc cacgcgggtg tccggcgtga cgagaaagac gatggcgctg accgtcgtga
    27901 tggcccgcac aaagctgtag atcagggccg agagaatggc cggacgcagc agcggcagta
    27961 aaatgtgggt tatggttcgc aaggaacccg cgcgcaggct gagtgaggct tcatccagcg
    28021 atttgtcgat ctgccccagg cctgcaatcc cggcgcgaat acccaccgga acgttacgca
    28081 tcaccatcga gataatcaca attgccgccg ttccggtgag ataaaccgga gcgctgttaa
    28141 aggcaagaat atacgaaacg cccgccaccg tacccggtac ggcaaagcac agcatggtgg
    28201 tgaactcgat ggtctttttg cctttgaatt gttgacgcac caccacccag gcgatgagca
    28261 ggccaaagat agcggtaatg ggagcggcaa tcccggcgta gagcagggta tccagcagtg
    28321 aaggccacgc gccgtcgctc attccctgac caaatagctt gataaagtta tccagcgtca
    28381 gggtgtagtc cacgccccag ttcaccgtga agctgccgta gaaaatgctg ccgtagagca
    28441 gggcgttaaa ggcaatccat atcgcaagta tggcgaccac gctccacacc agggtgaccg
    28501 gaagcggctg gacgtcgcca cggtaggatt tgccggaaac cgtgacgtaa gagcgtttac
    28561 caatccacat atactgtatg cagaacacca gcagcgagaa cagcagcagg aaagcgccaa
    28621 gggtgctggc ggcctgataa tccagctgcg agccggtaat atagaagtaa atttgcgtcg
    28681 ccagtacgtc gaagttaccg ccaagtacca gcgggttact gaaatcagcc agtgactgta
    28741 ccacgacgat taaaaaggca ttggcgagcg cgggcttcaa caacggcaca aacacgccgt
    28801 taaaggtttg ccagcggctg gcgcgcaggg tgtaggaggc ctcttccagc gagggatgaa
    28861 tggttttaat ggccccgtcg aggatcataa atgccattgg cgtgaatgcc agaacctgtg
    28921 ccagccagat cccggtaaaa ccgtacagcc agttggtgtt ggtcagccca aaccactcca
    28981 ccataaattc cgtaacatag ccggaacgcc ccatcatgag cgtcacgcct aagccgacca
    29041 caaacggtgg cgtaacgatg ggcaaaatgg aaaagatgcg accaataatg gcgctgcgtt
    29101 tagcgatgcg cgtggtataa atcgccagca ccaggccgaa gaaagcacag cctatcccca
    29161 ccgctatcga caagacgata gagttgacga tcacctgaac aatatgtgcc tgcgagagca
    29221 cggtcataaa cgccagcggg gcgagctctc ccgcgtcgtt ggtgaacatc gggataaaaa
    29281 tggcgatgct cggccagacg ataaaaatgc caatcagcgc cacaatcgca atcagtgagc
    29341 cgatcacgaa gcggtcgccg cccagccatt cgaggcgggt taaggccagc gtcataatcg
    29401 cgcctagttc gacaaacagc acgatggcgg cgtagcctaa accgcgtcca gccacggtgg
    29461 cgctgatgac gataaacgcc atacagagca atgcccagcc ggcatcgaaa taatgacgac
    29521 tacgctgttc gcgcgtggcc tgacgaaacg ggcggaccag caacaggctc gggagtaaat
    29581 accacagcca gctgatatta aaatgcgacc agccgtacgc cccgaggatc tcatcactcg
    29641 tggattccag caggccataa tcgaggctcc agctcggcaa caggacaaat gccagccagc
    29701 ccagcaaaac ccaaaggaaa atcgcatccc gctttttcac gggatggagt gcaagtgtct
    29761 gtgacatagc agttccggta tggttggccg gggcaggcgt ctgcgctgca cccggcaaat
    29821 gcgcctaatg cgggtttatt tacccatctt cacttcgctg acccatttat taatcaatgc
    29881 cttacgaaca tccgtggaac catatttatc catgtcgtag ttgattaatt tcaggtcgtc
    29941 gagcttcagc gaatttggcg acgtttcggc ggtggtatta gtcaggatct gataagactt
    30001 ccctttcttc cacgccagtt cctgagcttc ttttgataat acccagtcca caaacagttt
    30061 ggcgttgtcg aggttgcgcg cgcctttcag aatgctgacg ccgccaattt cgtagcccgt
    30121 accttcacac ggggaaatca actccagcgg cgcgccctgt tctttttcca gcgagtagtc
    30181 atgcaggaag ccaataccaa tcgccgtttc gccacgggcg gcgttacgtg ccggggctat
    30241 gcctgatttg gtgtactgag agacgttggc gttcagttgt tttagataat caaaggcctg
    30301 atcttccccc cacagctgcg caaaagtagc cagggcggtg taggcagtac cggaactttg
    30361 cggatcggca atttgaattt cgcctttata ttccggtttg gtgagatctt tccagcactt
    30421 cggcaccggc aggtttttct ctttcagacg atcggtgttt acgccaaaac cgaggatccc
    30481 cacataaacg gcagaggaca gattcccttt cacttttgcc ggatcgcgga atttctccat
    30541 gatctgctca aggttagggg atttatacgc ctgtagcagg cccatctcgc cagcctgcga
    30601 ttgcgggtct aaagtgccgc cgtaccagac gtcagcctgg gggtttttct tttcagcatc
    30661 gacttttgcc agggtgcttc cggaaccgtt gcggataaac gaggttttta catcatattt
    30721 ctcgccaaac gctttggttt cggcctcaca catttcattg gtggcgctgc aatagaccac
    30781 cagccggcct tttgcctgcg cggcacccgt taaagtcgcc agcgcaacgg cagaagcaat
    30841 cagagtagag gtaaaggtca ttttcattgt tttatccttc gagcgtgagt ggtggtgatg
    30901 cccgccatca gtaacggcat cagcagtaaa cccataagta ctgcggcgac cgagagtaaa
    30961 ctgaacattc ccggccagcc gtagcgttcg atgaccagcg acagcggcca gcctgccagc
    31021 gccgcgccaa ggtaagcgaa caggccgaga aatccggtga ttgaacccgc cgcagctttg
    31081 tgcccacact ccaccgccgc aagtccaatc agcatttgtg ggccaaagac gaaaaagccc
    31141 acggtaaaaa agcacactgc cagcagagcg tagtgatgaa ccggtgccag ccacagtgcg
    31201 gcgactgaca ccatcaaccc caacgtgaac agcaaaatca ttggcgctcg ttgcccgcta
    31261 aacagcaaat ccgatcccca tccggcaaat aacgcgccga gtaagccgcc cacttcaaac
    31321 agcattaccg tggcgttggc gctgagcagg ttgacaccgt ggctttccgt cagccagata
    31381 ttgccccagt cgttaagcgc aatccggatc acataaacca gtacatatga gacgcccagt
    31441 agccagatca gcggattttg cagcatggtg gtgcgcaaca tttgccacag ccccatcggc
    31501 ggactttgtt gttcctgacg taactccagc ggatcgtgac gccagtgacc aactgtcggt
    31561 aatccctctt cctggggcgt gcctttgagt tgtagcgtta accagatccc taatgccatg
    31621 ctgattatcc ccggtgtcag catcgcggcc tgccagcccc accagtgggc ggcaaaggcg
    31681 ctaatcagcg gaataatcgc tccgccaatg ttgatcgaca tattccagca gccccaccag
    31741 aaaccgcgct cattgcgtga gtaccagtgg gtcagcaagc gggcacacgg tggccatccc
    31801 cagccctgaa aaaaaccgtt cagtgtccag accacaagca gaagcgtaag tgattcgcca
    31861 aaggcaaaca ccacgttcaa taacccggtg gcgaacaagc cgacgcccat aaaaccgcgt
    31921 tgtccgtggc tgtcatgcca cagtccggcg gtaaatttcg acaatccgta gctgagataa
    31981 aacagcgaac ccagcaggcc aatgtcgcct ttatccagtc cgaggtcggt ttgtagcgcc
    32041 ggaaggacgt aattcacgct ttttcgcgtc agataaaatg cggcgtagcc aataatcatg
    32101 cacatcagca atcgcggacg taatgtccgg tagcattgat taatttctct cgctgaacgc
    32161 gcgtgcatgg ttgtctccat gttgctgact atatgaaaat gaaagtgaaa ggggatgaga
    32221 gaaaatccgg gatacctaag actttttcct ggttatccgg cgatttgttg caaaaatgtg
    32281 ggcaagttaa caattacccg cgtgccgtgc tgtttttcga gcgttaagtc gccgcccagg
    32341 gcgctgacgc gctcacgcat cccctgaatg ccaaatccag ccattttccc gctggcgatc
    32401 cccacaccgt tgtcgctgac ttccagatgc aatacctctc cctgctggcg caggataatc
    32461 gtcacttcgc tggcttcggc gtgtttacag atgttattga gcaactcctg caataaccga
    32521 tacaaggtaa agcgcaccgt ttcgttttct ggtgtggagt tcagttgata ggcaaactgg
    32581 caatgaatgc cgcgttcact aaaggcaaat tcattaatca gatgcagcaa cgcttcgcga
    32641 aatgtcagtt catcaagcgc gggaggccgc aactggcgca gcaattggcg ggtggagaga
    32701 tgaatgcggc gggcgagctc attgatttgg ctggctgcgg attgaatttg cgccggatca
    32761 cgcgcccgct tcaccagttg cgactggatc tgaatggcgg tgatgttctg accgatttcg
    32821 tcgtgcagct ctcgcgccag gttttttcgc gtgtcctcct cggtgtggat cagcttttcc
    32881 gccaatgccc gccgcgctgc cagctcggcc tccagccgtt gtcggtaatg gtgcaggttt
    32941 agcgccagat gttgctggcg actgatggca atgcccaggc caattcccag caaggcctgg
    33001 gtcgcgagga aaatctccag ctccaccagg ttactaaagc ccacgccaat ctggcgggcg
    33061 atggtgatca tcatgctgcc caaaagcccg gagagtacgc caccttgcca gccaaatttc
    33121 cacgccatca ccacattggg caaaaagacc acgatcaaca gcagacgttc aatttcaggt
    33181 gacagcacca tctgagtgcc gataccaata ataaaaaaca ggctacacca gatgattaaa
    33241 gaggtacgca aggggggatt atgcgtatcg agtcccagga gctggtagcg atgctgctgg
    33301 cgcagaaact caaagataag atagacaaac ggcgtcagta atacgccgcc ggtaaatgag
    33361 gtcaaacccg gtagcattat tgggcctgac ataaaagggg agagcagggc ggtattcaac
    33421 aatgaagctg ccgtgacagt ggctaacagc aacgtcagac gctgccagta aagtggaaaa
    33481 cggtgccaga agttttgcgc aaggagggca ggaaacaggc taataaccgg cgttgccagc
    33541 atgagataac cgttcaatag ctgttcacta tggagccaga acatcatcag caaagggggg
    33601 agcaccaacg caggccagta gcggcgagag agcaaaataa caagggcgag ataaactccc
    33661 tgcgggagaa acaacaccgc ctgttgaccg ttattggtca gataaaagct gagcgtccac
    33721 agcatcagcc agccggttcc ccaggctagc acaataaaca gcaaaatcac ccaatgacga
    33781 agattgcgcg acattaatgc cccgccagca gatggtggtc gagcgcaaaa tgtaccagtt
    33841 caatggtgct atggcagttc agttttccca gcacattggc gcgatgcaca tgtaccgttt
    33901 tatgactgag ttcgagttta aaggcgatct ctttaacgct gtctcctttg accagtaact
    33961 caaacacttc acgttctcgt ggggtgagaa tttccagcgc ctgggcgggt tgcccgccgc
    34021 cgcgtaaagc ccgaatcgca tcggcacaga gataatgccc gcccagcccg accgagcgca
    34081 ccgcctgtac taactcttcc ggcccacagc gtttggttaa atacccgctg gcaccggcat
    34141 ccagcgcgct ttgcacaaag gttggcgcgt cataaatgct aagaataatg gcgcgaaatt
    34201 gcggtttttg tgcacgcagg cgcttaagca aactgaggcc gttttcgtcc ggcatggcaa
    34261 tatcaatgac ggcaacattg acgtcatcac ggattaatgc cgaccatgcc tgcgcggcac
    34321 tactgtactg accaatgacc tccagatcgt cttccagaga gagtaattgt gcgaagccag
    34381 agcgcaccac aacatggtca tccaccagca ctacccgaat catacttttc ttctcttatt
    34441 atctaattct cctatcatgc atcacggcgc ggcgctggca atcaaccagc gacagatatg
    34501 gaatggatgt aacaaaatgg caataatttg ccggagcgcg cacatcttat ctggcttgtg
    34561 aaccggcggg atacgttcgc caaaaccgca aaatgcgcaa actcgctaac cattgccgat
    34621 ttttttctaa aaacgctttg ttcttaacaa gaccttttaa ttcctttatc aaactgaccc
    34681 gcaacgaaat actgctccca taacaagaca ggggagcaga caatcatggc aatttcatcg
    34741 cgtaacactc ttcttgccgc actggcattc atcgcttttc aggcgcaggc ggtgaacgtc
    34801 accgtggcgt atcaaacctc agccgaaccg gcgaaagtgg ctcaggccga taacaccttt
    34861 gctaaagaaa gtggagcaac tgtggactgg cgcaagtttg acagcggagc cagcatcgtg
    34921 cgggcgctgg cttctggcga cgtgcaaatc ggcaaccttg gttccagccc gttagcggtt
    34981 gcagccagcc aacaggtgcc gattgaagtc ttcttgctgg cgtcaaaact gggtaactcc
    35041 gaagcgctgg tggtaaagaa aactatcagc aaaccggaag atctgattgg caaacgcatc
    35101 gccgtaccgt ttatctccac cacccactac agcctgctgg cggcgctgaa acactggggc
    35161 attaaacccg ggcaagtgga gattgtgaac ctgcagccgc ccgcgattat cgctgcctgg
    35221 cagcggggag atattgatgg tgcttatgtc tgggcaccgg cggttaacgc cctggaaaaa
    35281 gacggcaagg tgttgaccga ttctgaacag gtcgggcagt ggggcgcgcc aacgctggac
    35341 gtttgggtgg tgcgcaaaga ttttgccgag aaacatcctg aggtcgtgaa agcgttcgct
    35401 aaaagcgcca tcgatgctca gcaaccgtac attgctaccc cagacgtgtg gctgaaacag
    35461 ccggaaaaca tcagcaaact ggcgcgttta agcggcgtgc ctgaaggtga cgttccgggg
    35521 ctggtgaagg ggaataccta tctgacgccg cagcaacaaa cggcagaact gaccggaccg
    35581 gtgaacaaag cgatcatcga caccgcgcag tttttgaaag agcagggcaa ggtcccggct
    35641 gtagcgaatg attacagcca gtacgttacc tcgcgcttcg tgcaataaaa ggaggcgcag
    35701 atgctgcaaa tctctcatct ttacgccgat tatggcggca aaccggtact ggaagatatc
    35761 aacctgacgc tggaaagcgg cgagctactg gtggtgctgg ggccgtccgg ctgcggtaaa
    35821 accaccctgc tgaatttgat tgccggtttt gtgccttatc agcatggcag cattcaactg
    35881 gcgggtaagc gtattgaggg gccgggagca gagcgtggcg tagtttttca gaatgaaggg
    35941 ctactaccgt ggcgcaatgt acaggacaac gtggcgttcg gcctgcaatt ggcaggtata
    36001 gagaaaatgc agcgactgga aatcgcgcac cagatgctga aaaaagtggg gctggaaggc
    36061 gcagaaaaac gctacatctg gcagctttcc ggtggtcaac gtcagcgggt ggggattgcc
    36121 cgcgcgctgg cggcgaatcc ccagctgtta ttactcgacg aaccgtttgg tgcgctggac
    36181 gccttcaccc gcgaccagat gcaaaccctg ctgctgaaac tctggcagga gacgggcaag
    36241 caggtgctgt tgattaccca cgatatagaa gaagcggtgt ttatggcgac tgaactggtt
    36301 ctgctttcac ccggccctgg ccgcgtgctg gagcggctgc cgctcaactt tgctcgccgc
    36361 tttgttgcgg gagagtcgag ccgcagcatc aagtccgatc cacaattcat cgccatgcgc
    36421 gaatatgttt taagccgcgt atttgagcaa cgggaggcgt tctcatgagt gtgctcatta
    36481 atgaaaaact gcattcgcgg cggctgaaat ggcgctggcc gctctcgcgt caggtgacct
    36541 taagcattgg cacgttagcg gttttactca ctgtatggtg gacggtggcg gcgctgcaac
    36601 tgattagccc gctgtttttg ccgccgccgc aacaagtact ggcaaaactg ctcaccattg
    36661 ccggaccgca gggatttatg gacgccacgc tgtggcagca tctggcagcc agcctgacgc
    36721 gcattgtgct ggcgctactc gcggcggtgt tgatcggcat tccggtcggg atcgccatgg
    36781 ggctaagccc gacggtgcgc ggcattctgg atccgataat cgagctttat cgtccggtgc
    36841 cgccgctggc ttatttgccg ctgatggtga tctggtttgg tattggtgaa acctcgaaga
    36901 tcttactgat ctatttagcg atttttgcac cggtggcgat gtcggcgcta gcgggggtga
    36961 aaagcgcgca gcaggttcgc attcgtgctg cccagtcgct gggtgccagc cgtgcgcagg
    37021 tgctgtggtt tgtcattttg cccggcgcgc tgccggaaat cctcaccgga ttacgtattg
    37081 gtctgggtgt gggctggtct acgctggtgg cggcggagct gattgccgcg acgcgcggtt
    37141 taggatttat ggttcagtca gcgggtgaat ttctcgcgac tgacgtggtg ctggcgggga
    37201 tcgcggtgat tgcgattatc gcctttcttt tagaactggg tctgcgcgcg ttacagcgcc
    37261 gcctgacgcc ctggcatgga gaagtacaat gagtgaacgt ctgagcatta ccccgctggg
    37321 gccgtatatc ggagcacaaa tttcgggtgc cgacctgacg cgcccgttaa gcgataatca
    37381 gtttgaacaa ctttaccatg cggtactgcg ccatcaggtg gtgtttctgc gcgatcaagc
    37441 cattacgccg cagcagcaac gcgcgctggc ccagcgtttt ggcgaactgc atattcaccc
    37501 tgtttaccct catgccgaag gggttgacga gatcatcgtg ctggataccc ataacgataa
    37561 tccgccagat aacgacaact ggcataccga tgtgacattt attgaaacgc cacccgcagg
    37621 ggcgattctg gcagcgaaag agttaccttc gaccggcggc gatacgctct ggatcagcgg
    37681 tattgcggcc tatgaggcgc tctctgttcc cttccgccag ctgctgagcg ggctgcgtgc
    37741 ggagcatgat tttcgtaaat cgttcccgga atacaaatac cgcaaaaccg aagaggaaca
    37801 tcaacgctgg cgcgaggcgg ttgcgaaaaa tccgccgttg ctacatccgg tggtgcgaac
    37861 gcatccggtg agcggtaaac aggcgctgtt tgtgaatgaa ggctttacta cgcgaattgt
    37921 tgatgtcagc gagaaagaga gcgaagcctt gttaggcttt ttattcgccc atatcactaa
    37981 accggagttt caggtgcgct ggcgctggca accgaatgat attgcgattt gggataaccg
    38041 tgtgactcag cactatgcca atgccgatta cctgccacag cggcggatta tgcatcgggc
    38101 gacgattctt ggggataaac cgttttatcg ggcggggtaa tacgagagtg gacggtcctc
    38161 tcgccccctt ggggagaggg ttagggtgag ggggcgttca ccgtgctttc aacaggttaa
    38221 ctcccccttt ctgagaggaa acaaaattaa cgcagaatct tcttctcagc caaatccagc
    38281 gcaaagtagc tgaaaatcag atccgcaccc gcacgcttaa tcgaacctaa gctttcgagc
    38341 acgactttct cttcatctat agcacccgcc agcgcggcga acttaatcat cgcatactca
    38401 ccgctcacct gatacgcgcc aatcggcaat tcagtacgtt cacgcagctc acgcacgatg
    38461 tcgaggtacg ctccagcagg tttaaccatc aggcagtctg cgccctgggc ttcatccagc
    38521 agtgactcac gaatcgcctc acgacggttc attgggttca tctgatagct tttgcggtcg
    38581 ccttttaatg cgcttccggc agcttcacgg aacggaccat aaaaggagga ggcgaacttg
    38641 gtcgaatacg acataatcgc cgtatcttta aagccagcag cgtccagcgc ctggcgaatc
    38701 gcctgtacct ggccatccat cgcggcggaa ggggcgatga agtctgcacc tgcagcagct
    38761 gcaaccacgg cttgcttgcc taaattttcc agagtcgcgt cgttgtcgac gccatgctcg
    38821 cacagcacac cgcagtgacc gtgagaagtg tattcacaga agcaggtgtc tgacatgaca
    38881 atcatttctg gcaccgtctg cttgcagatg cgcgacatac gcgctaccag tccatcttcc
    38941 cgccaggcat cgctgccggt ttcatcggta tggtgagaga tgccgaaagt catcacggaa
    39001 cgaataccgg cgttggcgat gcgttcaatt tcgcgtgcca gatgtttctc tggaatgcgc
    39061 atcacgccag gcatggcttc aacggctttg tagtcgtcaa tttcttcttc aacaaagatc
    39121 ggcaacacca ggtcgttaag gctaagtgtt gtctcttcaa acatagcgcg cagcgcagga
    39181 gatttgcgca ggcgacgagg gcgttggatt aagtctgtca tggtctgcct gatgtttgtg
    39241 gaattaaggt gcacagtata cctgaagcgg ggctggagtg ttttacgaaa gttgtcacga
    39301 tgttgacaag aagagaatga aagagagacc agggcatctt tgtaatagaa atagcaataa
    39361 cttcatattc actgaaaata ttttttaatt tttatttata ggtgctggtt attatttttg
    39421 tcaatttggt tgaactgtca aaaattga
//
LOCUS       DAZKIP010000028        39219 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZKIP010000028 DAZKIP010000000
VERSION     DAZKIP010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.028"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            74..1729
                     /gene="aslA"
                     /locus_tag="ACHOUK_001739"
     CDS             74..1729
                     /gene="aslA"
                     /locus_tag="ACHOUK_001739"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase AslA"
                     /protein_id="HFP9897209.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYLVKPATTIADNMMPVMQHPAQDKEPQQKLAELEKKTGKKPNVVVFLLDDVGWMDVG
                     FNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATILTGQYSIHHGILMPPMY
                     GQPGGLQGLTTLPQLLHDQGYVTQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMYT
                     EWRDVHVNPEVALSPDRSEYIKQLPFSKDDVHAVRGGEQQAIADITPKYMEDLDQRWM
                     EYGVKFLDKMAKSDKPFFLYYGTRGCHFDNYPNAKYAGSSPARTSYGDCMVEMNDVFA
                     NLYKALEKNGQLDNTLIVFTSDNGPEAEVPPHGRTPFRGAKGSTWEGGVRVPTFVYWK
                     GMIQPRKSDGIVDLADLFPTALDLAGHPGAKVANLVPKTTFIDGVDQTSFFLGTNGQS
                     NRKAEHYFLNGKLAAVRMDEFKYHVLIQQPYAYTQSGYQGGFTGTVMQTAGSSVFNLY
                     TDPQESDSIGVRHIPMGVPLQTEMHAYMEILKKYPPRAQIKSD"
     gene            complement(1897..3132)
                     /gene="aslB"
                     /locus_tag="ACHOUK_001740"
     CDS             complement(1897..3132)
                     /gene="aslB"
                     /locus_tag="ACHOUK_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFP9897210.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETTAREGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVSK"
     gene            complement(3279..3355)
                     /locus_tag="ACHOUK_001741"
     tRNA            complement(3279..3355)
                     /locus_tag="ACHOUK_001741"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3319..3321),aa:Pro,seq:tgg)
     gene            complement(3398..3484)
                     /locus_tag="ACHOUK_001742"
     tRNA            complement(3398..3484)
                     /locus_tag="ACHOUK_001742"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3448..3450),aa:Leu,seq:cag)
     gene            complement(3505..3580)
                     /locus_tag="ACHOUK_001743"
     tRNA            complement(3505..3580)
                     /locus_tag="ACHOUK_001743"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3545..3547),aa:His,seq:gtg)
     gene            complement(3639..3715)
                     /locus_tag="ACHOUK_001744"
     tRNA            complement(3639..3715)
                     /locus_tag="ACHOUK_001744"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3679..3681),aa:Arg,seq:ccg)
     gene            complement(3818..5203)
                     /gene="yifK"
                     /locus_tag="ACHOUK_001745"
     CDS             complement(3818..5203)
                     /gene="yifK"
                     /locus_tag="ACHOUK_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9897211.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(5394..6134)
                     /gene="wecG"
                     /locus_tag="ACHOUK_001746"
     CDS             complement(5394..6134)
                     /gene="wecG"
                     /locus_tag="ACHOUK_001746"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFP9897212.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEENAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTTVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(6137..7489)
                     /gene="wzyE"
                     /locus_tag="ACHOUK_001747"
     CDS             complement(6137..7489)
                     /gene="wzyE"
                     /locus_tag="ACHOUK_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFP9897213.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(7486..8565)
                     /gene="rffT"
                     /locus_tag="ACHOUK_001748"
                     /gene_synonym="wecF"
     CDS             complement(7486..8565)
                     /gene="rffT"
                     /locus_tag="ACHOUK_001748"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFP9897214.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFYWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDASLNTMANDRQREGKMTILVGNSGDRSNEHIAALRAVHQQFGDTVKVVVP
                     MGYPPHNEAYIEEVRQAGLELFSEENLQVLSEKLEFDAYLTLLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(8562..9812)
                     /gene="wzxE"
                     /locus_tag="ACHOUK_001749"
     CDS             complement(8562..9812)
                     /gene="wzxE"
                     /locus_tag="ACHOUK_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFP9897215.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISLGLFGNHDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAVMLIKRGAIPLSYLKPSWDNGLAGQLS
                     KFTLMTLITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPTHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(9814..10944)
                     /gene="rffA"
                     /locus_tag="ACHOUK_001750"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(9814..10944)
                     /gene="rffA"
                     /locus_tag="ACHOUK_001750"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFP9897216.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCLQNAHMFYIKLRDIGDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(10949..11623)
                     /gene="rffC"
                     /locus_tag="ACHOUK_001751"
                     /gene_synonym="wecD"
     CDS             complement(10949..11623)
                     /gene="rffC"
                     /locus_tag="ACHOUK_001751"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFP9897217.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPFLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNASDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASSRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(11601..12482)
                     /gene="rfbA"
                     /locus_tag="ACHOUK_001752"
     CDS             complement(11601..12482)
                     /gene="rfbA"
                     /locus_tag="ACHOUK_001752"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFP9897218.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEVATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(12501..13568)
                     /gene="rffG"
                     /locus_tag="ACHOUK_001753"
     CDS             complement(12501..13568)
                     /gene="rffG"
                     /locus_tag="ACHOUK_001753"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFP9897219.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPQGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWRKQVQDGSYQGERLGLKG"
     gene            complement(13568..14830)
                     /gene="wecC"
                     /locus_tag="ACHOUK_001754"
     CDS             complement(13568..14830)
                     /gene="wecC"
                     /locus_tag="ACHOUK_001754"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFP9897220.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(14827..15957)
                     /gene="wecB"
                     /locus_tag="ACHOUK_001755"
     CDS             complement(14827..15957)
                     /gene="wecB"
                     /locus_tag="ACHOUK_001755"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFP9897221.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(16013..17059)
                     /gene="wzzE"
                     /locus_tag="ACHOUK_001756"
     CDS             complement(16013..17059)
                     /gene="wzzE"
                     /locus_tag="ACHOUK_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFP9897222.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(17071..18174)
                     /gene="wecA"
                     /locus_tag="ACHOUK_001757"
     CDS             complement(17071..18174)
                     /gene="wecA"
                     /locus_tag="ACHOUK_001757"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFP9897223.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGNLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFLLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(18414..19673)
                     /gene="rho"
                     /locus_tag="ACHOUK_001758"
     CDS             complement(18414..19673)
                     /gene="rho"
                     /locus_tag="ACHOUK_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFP9897224.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(19758..19859)
                     /gene="rhoL"
                     /locus_tag="ACHOUK_001759"
     CDS             complement(19758..19859)
                     /gene="rhoL"
                     /locus_tag="ACHOUK_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFP9897225.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(20000..20329)
                     /gene="trxA"
                     /locus_tag="ACHOUK_001760"
     CDS             complement(20000..20329)
                     /gene="trxA"
                     /locus_tag="ACHOUK_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFP9897226.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            20460..21725
                     /gene="rhlB"
                     /locus_tag="ACHOUK_001761"
     CDS             20460..21725
                     /gene="rhlB"
                     /locus_tag="ACHOUK_001761"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFP9897227.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHCLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPVNQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            21861..23345
                     /gene="gppA"
                     /locus_tag="ACHOUK_001762"
     CDS             21861..23345
                     /gene="gppA"
                     /locus_tag="ACHOUK_001762"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFP9897228.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(23392..25413)
                     /gene="rep"
                     /locus_tag="ACHOUK_001763"
     CDS             complement(23392..25413)
                     /gene="rep"
                     /locus_tag="ACHOUK_001763"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFP9897229.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            25630..26079
                     /locus_tag="ACHOUK_001764"
     CDS             25630..26079
                     /locus_tag="ACHOUK_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFP9897230.1"
                     /translation="MAINFSPKVGEILECNFGNYPVSQNGPFSTTYYDGRIPPEMIKN
                     RLVVVLNGKINGNAFIVVPLSTTRDHDKLKRGMHVEIASNVINDLQFFDQQIRWAKTD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            26278..26559
                     /gene="ppiC"
                     /locus_tag="ACHOUK_001765"
     CDS             26278..26559
                     /gene="ppiC"
                     /locus_tag="ACHOUK_001765"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFP9897231.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(26605..28080)
                     /gene="ilvC"
                     /locus_tag="ACHOUK_001766"
     CDS             complement(26605..28080)
                     /gene="ilvC"
                     /locus_tag="ACHOUK_001766"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFP9897232.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVHTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDLNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            28230..29123
                     /gene="ilvY"
                     /locus_tag="ACHOUK_001767"
     CDS             28230..29123
                     /gene="ilvY"
                     /locus_tag="ACHOUK_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFP9897233.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(29175..30719)
                     /gene="ilvA"
                     /locus_tag="ACHOUK_001768"
     CDS             complement(29175..30719)
                     /gene="ilvA"
                     /locus_tag="ACHOUK_001768"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFP9897234.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(30722..32572)
                     /gene="ilvD"
                     /locus_tag="ACHOUK_001769"
     CDS             complement(30722..32572)
                     /gene="ilvD"
                     /locus_tag="ACHOUK_001769"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFP9897235.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHSDRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQISFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(32637..33566)
                     /gene="ilvE"
                     /locus_tag="ACHOUK_001770"
     CDS             complement(32637..33566)
                     /gene="ilvE"
                     /locus_tag="ACHOUK_001770"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFP9897236.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(33586..33849)
                     /gene="ilvM"
                     /locus_tag="ACHOUK_001771"
     CDS             complement(33586..33849)
                     /gene="ilvM"
                     /locus_tag="ACHOUK_001771"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFP9897237.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(33846..35492)
                     /gene="ilvG"
                     /locus_tag="ACHOUK_001772"
     CDS             complement(33846..35492)
                     /gene="ilvG"
                     /locus_tag="ACHOUK_001772"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFP9897238.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLKLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(35495..35545)
                     /gene="ilvX"
                     /locus_tag="ACHOUK_001773"
     CDS             complement(35495..35545)
                     /gene="ilvX"
                     /locus_tag="ACHOUK_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFP9897239.1"
                     /translation="MNNSTKFCFPRFRTGN"
     gene            complement(35632..35730)
                     /gene="ilvL"
                     /locus_tag="ACHOUK_001774"
     CDS             complement(35632..35730)
                     /gene="ilvL"
                     /locus_tag="ACHOUK_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFP9897240.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            36083..37603
                     /gene="yifB"
                     /locus_tag="ACHOUK_001775"
     CDS             36083..37603
                     /gene="yifB"
                     /locus_tag="ACHOUK_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFP9897241.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLVADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSTTVKQRVMAARERQFKRQNKLNAWLDSPEIRKFCKLESEDAQWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(37628..37966)
                     /gene="maoP"
                     /locus_tag="ACHOUK_001776"
     CDS             complement(37628..37966)
                     /gene="maoP"
                     /locus_tag="ACHOUK_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFP9897242.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            38085..38924
                     /gene="hdfR"
                     /locus_tag="ACHOUK_001777"
     CDS             38085..38924
                     /gene="hdfR"
                     /locus_tag="ACHOUK_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFP9897243.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(39020..39095)
                     /locus_tag="ACHOUK_001778"
     tRNA            complement(39020..39095)
                     /locus_tag="ACHOUK_001778"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39060..39062),aa:Trp,seq:cca)
     gene            complement(39104..39180)
                     /locus_tag="ACHOUK_001779"
     tRNA            complement(39104..39180)
                     /locus_tag="ACHOUK_001779"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39144..39146),aa:Asp,seq:gtc)
BASE COUNT         9589 a        10687 c         9899 g         9044 t
ORIGIN      
        1 cagggagagg gatctcaatt atcaatgctt aattacgtca tcattttgtt gacatgaaac
       61 cggagcattt tgcatggaat tttcgttttc acccaaactt cttgttgttg ctgtcgccgc
      121 cgctcttcct ctcatggcca gcgcagcaga taccccgtca actgccaccg cgcgcaaagg
      181 ctttgccgga tacgatcacc caaaccagta tctggttaaa ccggcgacca ctattgccga
      241 caacatgatg ccagtaatgc agcatccggc gcaggataaa gaaccccagc agaagctggc
      301 agaacttgag aaaaaaaccg gtaagaaacc gaatgtggtt gttttcttgc tggacgatgt
      361 gggctggatg gacgtcggtt ttaacggtgg cggcgtggcg gtgggtaacc ctacaccaga
      421 tatcgacgcc gttgccagcc aggggctgat tttaacttcg gcgtattctc aaccaagctc
      481 ttccccaacc cgcgccacca ttctcaccgg acaatactcc atccaccacg gcattctgat
      541 gccgccaatg tacgggcaac cgggcgggct gcaagggtta accacgctgc cgcagttgct
      601 gcacgatcag ggctacgtca ctcaggccat cgggaaatgg catatggggg aaaacaaaga
      661 gtcgcagccg cagaacgttg gctttgatga tttccgtggc tttaactcgg tgtctgatat
      721 gtacaccgaa tggcgcgacg ttcacgtcaa tccggaagtg gccctgagtc cggaccgttc
      781 tgaatacatc aagcaattac catttagcaa agatgacgtt catgcggtgc gcggcggcga
      841 acaacaggcc attgccgaca ttacgccgaa atatatggaa gatctggatc aacgctggat
      901 ggaatatggc gttaagttcc tcgacaagat ggcgaagagt gataagcctt tcttcctcta
      961 ttacggcact cgcggctgtc acttcgataa ctacccgaac gccaaatatg cgggtagctc
     1021 tccggcacgc acctcgtatg gcgactgcat ggtggaaatg aacgatgtgt tcgctaatct
     1081 gtataaagca ctggagaaaa acggtcagct tgataacacg ctgattgtct ttacctccga
     1141 taacggaccg gaagccgaag taccgccgca cggacgcaca ccgttccgtg gtgcgaaagg
     1201 atctacctgg gaaggcggcg ttcgcgtacc gactttcgtt tactggaaag gcatgatcca
     1261 accgcgtaaa tctgacggta ttgttgatct ggcagatctc ttcccaacag cactggatct
     1321 ggcggggcat cctggtgcga aagtggcgaa tttagtgccg aaaaccacct ttatcgatgg
     1381 tgtggaccag acatctttct tcctgggaac aaatggtcag tctaaccgta aggccgagca
     1441 ctacttcctc aacggtaaac tcgctgctgt gcgtatggat gaattcaagt atcacgtcct
     1501 gattcagcaa ccttacgctt atacccagag cggatatcag ggtggattca ccggcacagt
     1561 aatgcaaaca gccggatcgt cggtgtttaa cctctacacc gatccgcagg agagcgactc
     1621 tatcggcgtg cgccatattc cgatgggtgt accgctacag accgaaatgc acgcgtatat
     1681 ggagatcctg aaaaagtatc caccacgcgc gcagattaaa tctgattaag ccggcgctat
     1741 cgctgagatc tgcctttgcc ggatgcgatg ctgacgcatc ttatccagcc tacagaacgc
     1801 tgcaatttat tgaatttgca cgatcatgta ggccggataa ggcgtttacg ccgcatccgg
     1861 caatcaaccg caggcgacca caaaatggcc gcttttttac ttactcacca ccagcaaatg
     1921 cgcctgcata atgtcgctgg ccgggcgacc gtgcgccagc aaatcagcca ttgctttaag
     1981 atatggcggt agatggcgga aataacgctg atacccggca cacaaataat tcagtcccgg
     2041 tttgccgctg gcatcgagca tgaagcggtg tttcgggcag cctccccaac atgcttttaa
     2101 cacgttacaa ctgcgacact gcgccggtaa ttgcttaaat ttatcttcac caaacacctg
     2161 ctgttgcggg gaatcgatca tttctgcaat tgtttgctgg tgcatattcc ccagtcgata
     2221 ttgcggataa acatagtgat cgcaggcgta aacgtcgcca ttgtgctcaa caatcaccga
     2281 gcgcccacag gttggctgat gatggcaaac cgcacccggc gcaccgacaa aattggcaaa
     2341 cgcccattcg atattcatca cgaaaatctt gccgacgtcg cgtttaatcc agtggtcgaa
     2401 tatcgccacc agaaattcac caaattcatc ggggcgcaca gaccattccg tcagttcccc
     2461 ctgaatatca ccaggcgcat gtagtttcag tccttcgcgg gcagtcgttt catcagccag
     2521 gcgctcgacc accggaataa actggatgaa ttcgactccc gcatcgcaca aaaaatcata
     2581 aacctgtaac ggttgctgcg cgctggtgcg gttgacgcag accagcacgt tatagtcgac
     2641 atgatgtttt tgcaggagcg tcagggcacg catcaccagt ttatgcgtgg gtctgccgcc
     2701 ttttgtcacg cgatattgat tgtggatctc agccggacca tccagcgata acccaacaag
     2761 aaaatgattt tctgccagaa atgcacacca ttcatcatcg agcagtacgc cgttagtctg
     2821 gaagctgtta cttatcttcc tgccagcacc atatttcgcc tgtagcgcca cggcacggcg
     2881 gtaaaactcc agcccgagta gcgttggttc gccgccctgc caggtaaaag ccacttcgtt
     2941 ttgcggttcg ctggcagcga tatagtgacg gacatacgct tccagcgtgt catcgtccat
     3001 atgcgtgact ggcttttcgc ggtaaaggga ttgtttttcg agataaaaac agtagtcaca
     3061 gttcagatta caatcggaac cgctcggttt cgccatcaca tgaaaagcac gcgttggaac
     3121 ctgttgcagc atggtcgctc cttgcctgaa gtcatccttc aatacgttaa gggcgaaatc
     3181 ataccgtggg gcaatgagtt attacgggcg tttattgcga agggagggtg acgaaatgca
     3241 cagaaaacaa aaaaccccgc cgaagcgggg ttcaaaattg gtcggcgaga gaggattcga
     3301 acctccgacc cactggtccc aaaccagttg cgctaccaag ctgcgctact cgccgaaata
     3361 ctgctttttg aatttttagt tcaattcttt aaagtcgtgg tgcgaggggg gggacttgaa
     3421 cccccacgtc cgtaaggaca ctaacacctg aagctagcgc gtctaccaat tccgccacct
     3481 tcgcattgtc acaacttcta ataatggggt ggctaatggg attcgaaccc acgacaactg
     3541 gaatcacaat ccagggctct accaactgag ctatagccac cactacaaat cttgttacgc
     3601 ggtattacta ccaccgcagc tcaagcgccg ggactaaatg gcgcgcccga caggattcga
     3661 acctgagacc tctgcctccg gagggcagcg ctctatccag ctgagctacg ggcgcttagc
     3721 gccgttgcgg gggtggataa tacggacttc ccccccctct gtctagtccc tttttaaata
     3781 aattgcgcgt ttggttacgg tttgtgcgtt ttgctgctta ttcctccagt ttatgcgctt
     3841 tcccgtggcg attaaggcca aaaactttat aaatcgccgt caccgccagc ataaagatga
     3901 tgccaacaaa cagcgacata cgcgtatctt cattaaagta catgccgatc aaaacgcaaa
     3961 tcaggaatgc cattgttacg tagttggccc acgggaacag gattgagcgg aacggatggc
     4021 tggcaatcgc cgctttatgt gcacgccgaa aacgcagctg gcttatcaat atcacaaacc
     4081 atggcaccat ccccggaagc acgctggcac tgtagacgta gacaaacaca cgctgcggat
     4141 tgggaatgat gtagttcagg catgagccaa ttagcagaat agcaatagat actgccacac
     4201 ccgcaaccgg tacgccgtga cgggaaactt tcgccatcgc cgccggtaac tgacggtttt
     4261 tcgccagtgc gtagagcata cgtccgcaac tgtacatgcc gctgttacag ccagagagcg
     4321 cagccgtcag caccacaaag ttgataatgc ccgccgctgc ggtaataccg attttggcaa
     4381 aagtcagtac gaacgggctg ccgttgctgc ctatttcatt ccacgggaag atggtgacga
     4441 taacgaaaat cgcgccaacg tagaaaatca ggatccgcca cagcaccttg cctacggcac
     4501 tgcgcagcgt cacctgcgga ttcttcgctt caccggcagt aatgccaatc agctccacgc
     4561 cctggtagga cgccaccaca atacacagcg cggtcaggaa ccctttccag ccacccgcaa
     4621 agaaaccgcc atgctctgtg agattgctaa aaccaatcga ctgcccgcca ttgccaaagc
     4681 caaagaaaat cacgcccagg ccaatgacaa tcatcacgat aatcgtggtg actttgatca
     4741 tcgcgaacca gaactcgatt tcgccgtaca accgcaccgc tgccagattc gccaacgcca
     4801 ccagcgccac tgcgatcaat gcgggtatcc actgcgccat ctccgggaac cagaactgga
     4861 cataaacgcc aatggcggtg atttcagaga tccccaccgc catccacata aaccagtaag
     4921 accaggcggt gagatagcca aagaacgggc tcatataacg atgcgcataa acggcgaacg
     4981 aaccggtaac cggttcgagg aacaacattt cgcccattga acgcatgatg aaaaagacga
     5041 acagcccggc gatgatatag gccaacaata cggatggccc ggcccatttc agggtactgg
     5101 cggcccccat aaacaggccg acgccaatgg tgccccccag ggcgatgagt tcgatatgtc
     5161 gagcttccag cccacgctgt agctctggtt tgttatctgc cataaatcct cgtgttgtgt
     5221 ttgcatgctt tccggtgtta ccggttatcg ttatgggtac atcgagtgtt gcgaatgttt
     5281 tcgtaattca ggagaaatgg caaataaagc attaaaaatt tgaatgcttt gtgtaataaa
     5341 aaagcagaca ggcgacggag tgaccactcc gtcgctttac aaagagagga aaatcagagg
     5401 ttgccggtgt aatgccagcg taaataacgc agcaaacgaa gctgacgctt aatgcggctc
     5461 ggctgcgaaa gcaggcggta gagccactcc agccccagcg tttgccagat tttcggtgcg
     5521 cgttttacgt gaccggtgaa aacatcgtaa gtcccgccaa cgcccatata cagcgcatct
     5581 ggatgcacca gacggcagtc gcgcatgaag atctcctgct ttggcgatcc catcgcaacg
     5641 gtgacgattt gcgctccgct ggcatgaatg cgttcaaaca gcgcctgacg ctgctcgggt
     5701 ttaaaataac catcctgact gccaacgata ttcacattcc actggttgcg cagtttagct
     5761 tcagtttgcg ccagcacttc aggtttaccg cccacaagaa ataccgtcgt cccttctttg
     5821 cctgcgcgcg ccatcagctc ttcccagaga tcggcaccgg caacgcggga aacctgcgcc
     5881 tgcgggtact ttttacgtac tgaacgtaca acgctgatgc catccgcata tttaaattcg
     5941 gcagcgttaa ttaactccct gacctcggcg ttctcttcaa tggtcagcat tttttcagca
     6001 ttaatggcaa ccagcgttcc ctgcttaagc tgcccgtcag caaacagata atcgagggca
     6061 tgctgcatat cacgccaacc aatcaactgt aagccacgca gcgtataggt cggtgccgtg
     6121 gtgttgttat tcattgttat ccttcaacct gcgtccggag cgatgatttt gtacgtttat
     6181 gaatgagtcc ggcgctttca aaaagccagt acaacagttt tgcgatcatc agacatgcgc
     6241 cgaagaccac gataaaaaag accacgcgtg agacaaacga atccagccct tcacgcgcca
     6301 gcacgatcat attgaagatc gccccaaagc agaaactgtg caatatcgca gccttatagc
     6361 gattagtttc gcggttgccc agctcataca gccagtcgaa ccatttgatg atcagtccaa
     6421 ccacaatcgc cccgagcggg atgaacaacg cgccgcccat caccaccagt gagcctataa
     6481 gcgtaggcga gatcgccagt ccggagtggt tattcagcac ttcccaggta aagtagttgg
     6541 ctgagttcag caccatactc gggcgacccg gccacagcca ggaagggata aagacataga
     6601 aatcgcggac aattggcgcc aggccctgga agtcgatgtt gtcgtagttc tgcaacagca
     6661 acgccagatt ctcccacggc gagaaggtgt cgcgagtgag atagagaaac gtatagaacg
     6721 cttcatcgcc gctcacattc attccatagc gttttagcgc cagccagaac atgccaacaa
     6781 tccccagcac gcccgccgcc gccagcatcc acaacgaaat ccagccgcga ataatgccaa
     6841 taaacaggaa gatagcgaat gcgatgatga tattggcgcg agtgccgccg acaatcatat
     6901 aagtcagcaa gccaaaggcg accgtgctga cgaggaaaaa cagccacgct ttgctgtcct
     6961 ggcgcagaaa gtagaccacc agcatcgccg ggatgaaaaa gtaaaagaag cgttttaacg
     7021 ccacgccgga gacttcactg gaaaagatct gactgtagga gttaagccgg aacagcaaaa
     7081 agccgttgtg catgaagaag atgccgacgc ttaccagcgc gatacccatc aggatcaccc
     7141 acgtaagatt ggtttccacg cggttcatgg taaacagcgg acggcgcggt acatcagcaa
     7201 cgcgtttgcg taggcgggtt ttgtaggtga cgtagtaaac cgcgtagaag cagcccgcag
     7261 aaagcaacgc ctgcaacagg atttctggcg gcgcgacacc aacatcaaag cgaaacacca
     7321 gcacgctggt cagcgggaag ccaaagaaaa aggtgagcag aaacagcaat gaaaagaata
     7381 cattgaagtt aaagcgcaca cgacgaaact caaaccaggt cagcgtggca ataaacagcg
     7441 tgcagagcag ccagacaaca aacaggccac tgaattgcag cagactcatg cgacctcccc
     7501 ggcggcaatc gccaacgccc gctgccagcc ttgtagatag ttagggctaa agaaggcaat
     7561 ggtgttttta tccaccgacg ccaactggcg ctgcgcttca cgcacaatat cctcgttgag
     7621 atcgtcggta gtaaacagca ccggtaaatg ctgttccgtc atatcctgcc agaacggatt
     7681 ttcccggtta agcacacaag gaatgcccgc ctgaatcagt aagcacagcg taccaatgcc
     7741 ctgctggcgg gcaaaaataa agtaaccaag atcgcactga cgaagtagcg tcagataggc
     7801 gtcaaattcc agtttttcac tcagaacttg cagattttct tcgctgaata actccagccc
     7861 cgcctgacga acttcctcga tgtacgcttc gttatgaggc ggatatccca tcggcaccac
     7921 cacttttacc gtatcgccaa attgctgatg aacggcgcgc aaggcagcaa tatgctcatt
     7981 gctgcggtcg ccggagttcc ccaccagaat ggtcattttc ccttcacgtt gccgatcgtt
     8041 cgccatcgta ttgagagaag catccatccg cgtcgggaag tacagcagtt cgccccgcac
     8101 ctttgggtgc gttttggcaa aaaagctcaa atcaccacgg gtggcaaata cacagccgac
     8161 tcgcttttgc gccaggcgac gtagtgggta aaaaagctta tatctcaagc cactggaaag
     8221 ctcgtacagg tctgcccccc agatatgcca gtaaaactgg ctgggcttaa tgccaccact
     8281 cagcagagcc agccacagtg tgggattgaa ctgaccgtgg aagaaaaaac gctgctgacg
     8341 gttagctttt gctttcgcga tgaccgcttc cgccagcgat tttttcccag ggaaaaattg
     8401 cacagaaagc gccggacagc tatcacttaa gccgtcgtcc ttgccaacaa ccataaactc
     8461 gcgcgcgtgc tcgctcgtcg cggccagcgc gtcattgaaa aaccgcaaaa cggttcggtt
     8521 atggtgaggg atatccgatc ccagtacgtg aatcagtaca gtcatgcccg cctacgccag
     8581 agtaaaaaca cgccacaaca aagagaaaaa tagacgatat aggttgccat atatgcctgt
     8641 gccgcgccca gcgcgccgtg cgtagggatt agccagtggg caaataccat caataaagtg
     8701 aactggctga cttccgccag aatataaaac cgcagtgaag ctttggcgat caccagataa
     8761 ccaaagacat aagcgcccac ttttaacaca tcacccacca actgccaggc aaagagatcg
     8821 cgcatagcgg taaatttatt cgacaacagc agccagatag caaaatcacg cagcaaccag
     8881 acggtaaaac tcgccgccgc cactgccggt aagacgaatt tcagcgattt aaccacttcc
     8941 cgggtgatat cgcgcttttc cgttagccgc gacaacgtgg gcagcaaata tacgctgaac
     9001 gatgccgtaa taaattgcag gtaggcatcg gaaatactgc tcaccccttg ccagatcccc
     9061 acctcatccc agctatactg cgccgccagc agtttacgca tcatgatgta agcaacaggc
     9121 aaggtcaccg acgtaatcaa cgtcatgagc gtaaatttgc tcaactgccc tgccagaccg
     9181 ttatcccagc tgggtttcag atagcttaac gggatagcac cacgcttaat taacatgacg
     9241 gcggcaggaa tcaccaccag cgcgggaatc aacgccagac ccagcaacgc cccttcatat
     9301 ccgcccaaac ggtaactgac gtagtacgca agaacgccaa tcaagctgcc aacaatcaga
     9361 gataacgcat tacctgcggc atcgcgaaag cctttcatca acgccagtaa caggttgccc
     9421 caggcgatcc ccatttgcac cagcgccact aaacgcacca gcccctgata gtcgtgatta
     9481 ccaaacaatc ccaagctgat tggcgcagct gccagcacaa aaaccagcgc catcagcgta
     9541 gagaagccaa gtaccatcgc tgatgaagtg ccgaccacgc ggcgcagctg ttgcggatta
     9601 tcatggtact gggcaacgta tttggttaca ccgttaaaga tgccagcccc ggcaagcacg
     9661 ccgagcacgg taatcaactg gcggaaattt gccgccagcc caagccccgc cggaccaaat
     9721 gacaccgcca gcaacttacc gaccagtaac ccggcaccaa tcttgaccag tgtactggcc
     9781 gccgtccaca aggacgcttt tgccaacgac atatcaggag aagtagttca acaaagtcgc
     9841 aattaccgta cgctgattga cgggcgacag gttgtagaac agcggcaggc gcagcaggcg
     9901 ctcgctctct ttggtggtgt agcgatcttc accgtggaac tcaccaaagc gttcccccgc
     9961 agggcaaccg tgcagcggaa tgtaatggaa caccgccatg atttccgctt ctttcagaaa
    10021 gttaatcaac gcgctccggt caccaatatc ccgcagttta atgtaaaaca tatgcgcgtt
    10081 ctgcaagcag ccatcgggaa tcgacggcag ctcgatacgc ccggctttcg ccagaggtgc
    10141 taacgcatcg tagtagtttt gccacagcgc cagacgttgc tggttgatac gatccgctgc
    10201 ttccagttgc gcccacaggt acgcagcttg cagatcggac atcaaatagc tggagccgat
    10261 atcgcgccag gtatatttat cgacctgacc acggaagaac tggctgcggt ttgtgccttt
    10321 ttcacggatg atctcggctc gttcgatcaa cgctttatcg ttaatcagcg tcgcgccgcc
    10381 ttcaccgccc gccgtgtagt ttttggtttc atggaagcta aagcagccaa tatgaccaat
    10441 ggttcccagt gcacgccctt tgtaagtgga catcacgccc tgagcagcat cttccaccac
    10501 aaacagatta tgctttttcg ccaacgccat aatggtgtcc atttcgcagg ccacgcccgc
    10561 gtaatgcacc ggcacgataa cgcgcgtttt gtcggtgatc gccgcttcaa tcaacgtttc
    10621 gtcgatgttc atggtgtccg ggcgaacatc cacaaaaacg atttttgcgc cacgcagcac
    10681 aaaggcattg gcggtggaga caaaggtgta gctcggcatg atcacttcat cgccaggctg
    10741 gatatcgagc agcagcgccg ccatctccag cgaagcggtg caggacggcg tcagtaacac
    10801 tttggcgctg ccaaaacgtt gctccagcca ctgctggcag cgacgggtaa aaccgccatc
    10861 gccacacagt ttgccgctac ccattgccga ctgcatatag tcgagttcgg ttcccaccac
    10921 cggcggtgcg ttaaatggaa tcatgtgatc acctgtataa ccagtacgcg gtgctttcta
    10981 cattcgcacc actttgtatg tatcgtttaa gcgcggcggt gttgcccatt tgggtcgcca
    11041 cccgcaaagt tgttttaccg cgagcatacg cccagtttag cgccgtttgc atcagctcag
    11101 cacctgcacc gcgtccagcc agcaggccaa ttcgcgcatc tgtagcattg agttcccgta
    11161 aagagacata accgcgaata tcgccggacg ccgcacgtaa aatcagacat tgatgatcaa
    11221 aggtgccgcg cacggcattt tcaatccact gtgcataaaa gcgactgctg gcgtcaggcg
    11281 cataccacgg cgcacgaaaa cggctttgcg caaatgcggc gctggctaac tgacgtaatg
    11341 cgggaatatc ggtctcttgt gccactacag caccgctatc actggcattg ttcacgggta
    11401 gcgccaaatc aacttcacct tctaccaggg agaatcccag ctgttgcagg gcatccagtt
    11461 cccccgtatt tgatgccgca attttggcct gcacccgtga ccacggcgct aacgcgtctg
    11521 gcgtcaggaa cggtgcttca gacgtaatgc gcacgatggc gctgttaaca ccaaagaagg
    11581 cgttttccca ggttagtggc tcaatactgg cgcggacggg cacgaagtaa ctccagcaga
    11641 tattggccgt agccagtttt cgctaatgaa ctggcagcac gcttcacacc ctcgtcatcg
    11701 agccagccgt tacgccaggc gatctcttcc aggcaggcaa tcttaaagcc ctggcgtttt
    11761 tccaccgtct gtacaaaggt gctggcttca atcaggctgt cgtgagtgcc agtgtccagc
    11821 caggcaaatc cgcgcccgag cagttcaacg gtcaggttgc ccgcctcgag gtacatctgg
    11881 ttgatggagg taatctccag ttcaccacgc tccgacggct tcacctgctt tgcgtactcc
    11941 acgactttac tgtcgtagaa ataaagcccg gtcaccgccc agtttgattt cggctgtttt
    12001 ggcttttctt ccagcgagat agcgcggaaa ttatcgtcaa actccaccac gccaaagcgt
    12061 tccgggtcca tcacctgata gccaaaaacc gtcgccactt ccgtgcgcgc cgcaacatga
    12121 cgcagcttcg gactgaagcc ctgaccgaag aagatgttat cgcccagcac cagacaagaa
    12181 ggttcaccat taaggaaggt ttcaccgatg ataaaggcct gcgccagacc gtccgggctg
    12241 ggctgttcgg catattccag ctggataccg aactcactac catcgcccag caggcgctgg
    12301 aaataacctt tatcttccgg cgtagtgatg atgagaattt cgcggatacc ggccagcatc
    12361 agcaccgaca gcggatagta aatcattggc ttatcgtaaa tcggcaacag ttgcttcgat
    12421 acgccgcgcg taatcggatg caatcgggta ccggaaccgc ccgccaggat aatacctttc
    12481 atgtttgcct ccgctgaaaa ttagcctttc agacctaaac gctcgccctg atagctgccg
    12541 tcctgcacct gcttcctcca gctttcatta gccagatacc actgcaccgt tttacgcatt
    12601 ccactttcaa aggtttcctg cggcagccag ccaagttcac gggcaatttt cgaagcatca
    12661 atggcatagc gcagatcatg ccccggacgg tcagcgacaa aggtgatcaa gtcacgataa
    12721 tgcaccacgc cttgcggctt gttcggagcc agttcttcca gcagctcgca aatggtttcc
    12781 acaacatcga gattcttacg ctcgttgtga ccaccaatat tataggtttc accgactttc
    12841 ccggtggtcg ccacgcaata cagcgcgcgg gcgtgatctt caacatacag ccagtcacgg
    12901 atttgctgcc cgttgccata taccggtagc ggtttacccg ccagcgcgtt gaggatcatc
    12961 agcgggatca gtttttccgg aaagtggtaa ggaccgtagt tattcgagca gttggtgata
    13021 agcgttggca gaccataggt tcgcagccag gcgcgcacca gatggtcgct gctggctttt
    13081 gacgcggaat aagggctgct cggcgcatac ggcgtggttt cggtgaagaa atcatccgtc
    13141 gagtgcaggt caccatatac ttcgtcagtg gagatatgat gaaaacggaa cgctgatttt
    13201 ttatcttccg tcagcgcatt ccagtaagcc cgcgccgctt caagcaatgt ataagtcccg
    13261 acaatgttgg tttcaataaa cgctgccggg ccgtcaatag aacggtcaac atggctttcg
    13321 gctgccagat gcatgacaca gtctggctga tgctcagtga atacgcgcgc cagttctgcc
    13381 cgatcgcaga tatcaacttt ctcaaaggca aagcgctcgc tttgcgcgac cggtgccagc
    13441 gacatcaggt ttccggcgta ggtcagctta tcgaccacta ccaccgcgtc gctcgtttcg
    13501 ttgatgatat aacgcaccag cgccgagcca ataaacccgg caccgcctgt tatcagaatc
    13561 tttctcatca gcgccagact cctttagcat cgacgacata ctgctgatgg acattgtcgc
    13621 cattgataac tttgaactga ctatgatcga ccagcatcac cagcacgtct gccgttgcca
    13681 gcgcctcgtc aagctgcgcc agagtacaaa gcccggtcag ttttttcggc aactggtgga
    13741 tgttaggctc aacaaccaga gtttcgccgc tatgccactg ggcgatcagt tcagcgattt
    13801 ccatcgccgg gctttcgcgc aggtcatcaa tattcggttt aaacgccaga ccaaagcagg
    13861 cgattttcag ttcactggcg cgtttatcgg tagccgccag gcaatcagcc accgccgctt
    13921 tcacctgatc gataacccag aacggtttgt gatcgttcac ttcgcgcgcg gtacggataa
    13981 gccgcgcctg ctgggggttc tgtgccacga taaaccacgg atcaacagca atgcagtgac
    14041 cgcccacgcc agggccaggc tgaagaatat taacgcgagg gtgacgattc gccaggcgaa
    14101 tcagttccca gacgttaatc ccctgatcgg cacaaatcag cgacaattca ttagcaaaag
    14161 cgatattcac atcgcggaag ctgttttcgg tgagcttaca catttccgcc gtccgcgagt
    14221 tagtgacgac acactcacct tcgaggaaaa ttttgtacag ttcgctggcg cgggccgaac
    14281 aaaccggcgt cataccacca atcacgcgat cgtttttaat cagctcgacc ataacctggc
    14341 ccggtaacac gcgttccggg cagtaagcaa tgttgacgtc cgcctgctcg cccacctgct
    14401 gtgggaaagt gagatccgga cgcatctctg ctaaccattc tgccatcttc tcggttgacc
    14461 ccaccggcga ggtggattca aggatcacca gcgcgccttt tttcagcact ggcgcaatgg
    14521 agcgagcagc cgattcaacg taggtcatat ctggctcatg atcgccttta aacggcgtgg
    14581 gtacagcaat cagccaggca tccgcttcaa ctggcgtcgt gctcgctcgt aaaaaaccgc
    14641 cttctacggc agtttttact acgctcgcca aatcaggctc gacgatatgg atttcgccac
    14701 gattgatggt atcaaccgcg tgttggttga tatcgacacc aatcacctgt ttttgccgtg
    14761 aggcaaacgc tgctgctgta ggcagcccga tgtaacccag tccgataaca gaaatggtcg
    14821 caaaactcat agtgatatcc gattattttt taacgcttcc agaatgcgag agcatgcctg
    14881 accatcacca tacgggttat gggcgcggct catagtttga tattcgtttt cgtcttttaa
    14941 aagacgcgtc acttcctcga caattcgctg cttatccgtg cctaccagac gcaccgtacc
    15001 cgccgtcacc gcttccggac gctcagtggt atcgcgcatc accagcaccg gtttccccag
    15061 cgaaggcgct tcttcctgaa tgccgcctga gtcggtcaaa atcagccagg cgtggttcat
    15121 cagccagaca aacggtaaat actcctgagg atcgatcaga atgacatttt tcacatgccc
    15181 cagaatgcga ttgaccggct ctctgacgtt cgggttgaga tgcaccggat agacaatctg
    15241 gatgtcctgg tgcgtggtgg cgatgtctgc cagcgcgtgg cagatttctt caaagccacg
    15301 accgaagctt tcacgcctgt gaccggtcac cagaatcatc tttttatcgg ggtcgataaa
    15361 cgggtaattt gccgcaagtt ctgaacgcag cgtgtcgctg ctcatcacct ggtcacgcac
    15421 ccataacagt gcatcaatga ctgtattacc ggtaatgaag attcggctat ctgcaacgtt
    15481 ttcacgcagc aagttttgcc gggaagtttc ggttggagag aaatgataca tcgccagatg
    15541 cccggtcaat gtacggttag cctcttccgg ccacggcgaa tagagatcgc ccgtgcgtaa
    15601 accagcctca acgtgaccaa caggaatacg ctgataaaac gccgccaggc tggttgccag
    15661 cgtcgtcgtt gtgtcgccgt gaaccagcac gacgtctggt ttgaactcgg caagaatagg
    15721 ttttagccct tccagaatcc gacaggttat ctctgtcagg ccctgtcctg gctgcattat
    15781 gttgagatcg tagtcaggta caatggaaaa gagtttcagc acctgatcga gcatctcccg
    15841 atgctgcgca gtgacgcaaa ctttagcctc aaaaaaagga tcttttgcca acgcatgcac
    15901 caacggagcc atcttgatgg cttccgggcg cgtaccaaat acagtcagta ctttcacatc
    15961 gattctcttc gaataagcgg cgagcgcctt tgcgctcacc gcagcagtgt tgctatttcg
    16021 agcaacggcg ggttaatgcg acaccagccc cgatcagccc cccgacaatg ccccacataa
    16081 tcatcaggaa ggcacgacgt gggctatcgc gttttaccgg ttcttccggc gtacgcaaat
    16141 agcgataggt ctgaaaacgc ggatccaggg ttggaccaac attcagggtg tttaacatgg
    16201 cccgattctg atcatagtcg agatcaaagg ccggaccgac ggcctgtaaa ttttccagtc
    16261 gagcctggag cattggacgc ccaagcagga acatttctga atcaggtaat tcctcggcag
    16321 gcacatctgt cgcactgcgc gaaatattat gctgctcagc aattttcagc gcctgctcaa
    16381 tgctgttcat ccggcggtcg tagatggctt tcgccacctc ttcctgacgc ttcacctgtg
    16441 ctttcatctg gatggtacgc gccgcccatg cgcctttcag ctcatcattc agatggctgg
    16501 ctgcacgctg gctggcaaaa gcaacatact gacgtaacag gttattagcg tcaggcgcgg
    16561 tttcggcaat aagcttcacg ctgtcattga ccgcgcgggt aaagtctccg gggataaact
    16621 ggatgttgtt aatcatttca tccagcaacg ccgcatcggc tttgctgttg cccaccatcc
    16681 gctgcttgta atagtcggtt tgcagccaga actctctgcg ggtatcccac gaggccagtt
    16741 gcataacaaa ctctttatag gcttcgtcca tgaccgatgg ttggtcggca gaagccatgt
    16801 ttgaacggac atccaggtta cgcaaaaatt gctgctgcga gtaatatccc cccagcatat
    16861 tcaccgttgg acgatcggta atcgccgtcg agctccactc ctgacgagca aaaaacgtat
    16921 acgccagcgc gattaacgca aacgccagcc ccatgccaat aatccatagc ttcccagccc
    16981 acaaggtacg aaacaaccca cgaatatcca gttcattttc agcgtcttcg gccggtttcc
    17041 caggcattgg ttgtgtcatc acatcctcat ttatttggtt aaattggggc tgccaccacg
    17101 atttctacgc agtctgcgtt ttacgcgctt aataaagcga gcaactttcc aggcacgctt
    17161 aatgcaatat ccatagagga ggaatgctag caaaaagagc accaacatga cccactccgg
    17221 gacaaaatga gaatattctg ccagcacgcc aatggaagcg agcagtgctg cggcaagggt
    17281 aatcagcaca aacgcctggc gggaagtaaa cccggcacgc atgatcaaat ggtgaatatg
    17341 ctgacggtca ggagagaatg ggctcatgcc tttacgcagg cgacggtaca taatcgccac
    17401 catatccatt agcggaatgg cgattatcca caaagcggta accgggctga tgggatgggt
    17461 tttgccttgg gtcgtttcga gcaggatcca gatcacggta aaaccaatca gcgtactgcc
    17521 cgcatcaccc ataaagactt tgtagcggcg acccaggata ccaaggttaa gcatgatata
    17581 tggcaggatg gcggcgatca tcgcaaagca ccagattgcg aggctggttt gcccgtcgaa
    17641 ccacaaaatc ataccgattg ctgcaaacga gacgcaggac aacccgccca gcaagccatc
    17701 aatgccatca accatgttga acgcattaat ggccgcccag acggcaaata gcgtcaggaa
    17761 gtaaccaaac ggtccgagca ccatctccca ggagccaaag atataaccca ggctactgag
    17821 ataaaggttg ccaaacacca tcataacaat gccaacagcg gcctgtatgg tggcacggat
    17881 ttttacgctg atatcaaaac ggtcatccag cgcgccaatg aaaacaagca caccggcaca
    17941 agcgagatag agagatgcat gcggaatata gtaatcgaca attccgaacg tgaagcaaat
    18001 ccctgcgtaa accgaaatac ccccaacgag tggtatcaac ccctggtgac gtttgcggaa
    18061 gtttggttta tccactaaac cgactttttt tgccacctta cgggcaaaaa acagaaacag
    18121 tgtcgtgaat aaaaaaatac tgatgagatc agtactcact gtcagtaaat tcacaatgca
    18181 tgctctcaga gaaaattatt agcagaagta taaccacgaa gacctttatt cagaagggaa
    18241 agtccgttcc gaacccacca gtcctgtgca aaaattaacc atttgatatg caaattgcat
    18301 aacatgccag caaattccag gaattttcct aacatcgcca gcgcggcatt aagattacag
    18361 atataaaagc aaaacgccac gtaaacacgt ggcgtttttg gcataagaca aatttatgag
    18421 cgtttcatca tttcgaagaa atcgtcattg gtcttggtca ttgccagttt attaatgagg
    18481 aattccattg catcgatttc gcccatcggg tgaatgattt tgcgcaggat ccacattttc
    18541 tgcagttctt cctgagtcgt gagcagctct tctttacggg taccggaacg gttgtagtcg
    18601 atagccggga agacgcgttt ttcagcgatc ttacgagaga ggtgcagttc catgttgcct
    18661 gtacctttaa actcttcgta aataacttcg tccattttag aaccggtatc gataagcgcc
    18721 gtcgcgataa tggtcaggct gccgccctct tccacgttac gcgccgcacc gaagaagcgt
    18781 ttcggacgat gcagggcgtt ggcatccaca ccaccggtca acactttacc tgacgccgga
    18841 acaacggtgt tgtaagcgcg cgccagacga gtgatggagt cgagcagaat gataacgtct
    18901 ttcttgtgct caaccaggcg tttcgccttc tcgatcacca tttccgcaac ctgaacgtgg
    18961 cgagatgcgg gttcgtcaaa ggtagaagca acaacttcac cttttaccag tcgctgcatc
    19021 tcggttactt cttccggacg ttcgtcgatc agcagaacca tcagcacaca atccgggtgg
    19081 ttgtaagcaa tgctctgagc aatgttctgc agcagcatgg ttttaccggc tttcggcggt
    19141 gccacaatca gaccacgctg accacgaccg ataggtgatg ccagatccag tacgcgagcg
    19201 gttaaatctt cagtagaacc gttaccacgt tccatacgca gacgagagtt tgcgtgcagc
    19261 ggggttaagt tctcaaagag gattttgttg cgggcgtttt caggtttgtc gaagttaact
    19321 tcgttaactt tcagcagcgc aaaatagcgt tcaccttctt tcggcgggcg aatcttacca
    19381 gagatggtat caccagtgcg gaggttgaaa cggcggattt ggctagggga aacgtagatg
    19441 tcatcaggac cggcgaggta ggagctgtct gcggaacgga ggaaaccaaa tccatcctgc
    19501 aatatctcca gtacgccatc accaaagata tcttcgccac tctttgcgtg ctgcttcagg
    19561 atggcaaaaa taatgtcctg cttacgcata cgagccaggt tttccagccc catattttcg
    19621 ccgagagtga tcagctcaga aaccggcgta ttctttaatt cggtaagatt catagtggtg
    19681 tgagttctta aacttggggt aaatctcgaa cttaatgttg tgaatggtat ggcaggatca
    19741 tccatgcctg tttacggtca tcgactcatg tcttttcgct gcctggtcac aggagagtac
    19801 gcagaactga aacgacaaga cggattgagt gacgagccag aaatctgttc acttcgcatt
    19861 taagataaaa cgggaagcgt tggaaattac aagattcaaa cttaataagg tatgtttaat
    19921 acgaagtcaa cactaagtta gcatgactca cgccgggcgt ccagttttta gcgacggggc
    19981 acccgaacat gaaattccct tacgccaggt tagcgtcgag gaactctttc aactgacctt
    20041 tagacagtgc gcccactttg gttgccgcca cttcaccgtt tttgaacagc agcagagtcg
    20101 ggataccacg gatgccatat ttcggcgcag tgccagggtt ttgatcgatg ttcagttttg
    20161 caacggtcag tttgccctga tattcgtcag cgatttcatc cagaatcggg gcgatcattt
    20221 tgcacggacc gcaccactct gcccagaaat cgacgaggat cgccccgtcc gctttgagta
    20281 catccgtgtc aaaactgtcg tcagtcaggt gaataatttt atcgctcata tataactcca
    20341 caggaataag cctggcgtgt tggtttcgtt gttggtgtaa cattaaccaa ctaaaggttg
    20401 actttatttc accggatacg ctttcgtaaa gcaatagtaa gctgatattc taccacacta
    20461 tgagcaaaac acatttaaca gaacagaagt tttccgactt cgccctgcat ccgaaggttg
    20521 tagaagccct tgaaaaaaaa gggtttcata actgtacgcc cattcaggca ctggcccttc
    20581 cgctgacgct ggcgggtcgt gacgtagccg ggcaggcgca aaccggtacc gggaaaacga
    20641 tggcgtttct tacgtcaacg tttcattgtc ttctctctca tcccgcgatt gccgatcgca
    20701 aggtgaatca gccgcgtgcc ttaattatgg caccgacgcg tgaacttgcc gtgcagatcc
    20761 atgccgatgc ggaaccgctg gcggaagcta ccggcctgaa gctgggtctg gcttacggtg
    20821 gtgatggtta cgacaaacag ctgaaagtgc tggaaagcgg cgttgacatt ctgattggca
    20881 ccacggggcg tttaattgac tacgccaagc agaaccacat taacctcggt gccattcagg
    20941 tagtggtact ggacgaagcc gatcgcatgt acgatctggg ctttattaaa gatatccgct
    21001 ggctgttccg ccgtatgccg cctgtaaacc agcgcctcaa catgctgttc tccgccacgc
    21061 tttcgtaccg ggtacgtgaa ctggcgttcg agcagatgaa caatgccgaa tatattgaag
    21121 tggaaccgga acagaaaacg ggccaccgta ttaaagaaga gcttttctac ccttctaacg
    21181 aagaaaaaat gcgtttgctg caaacgctga tcgaagaaga gtggccagac agagcgatta
    21241 ttttcgccaa taccaaacac cgttgtgaag agatctgggg ccacctggcg gcagatggtc
    21301 atcgtgtcgg tttgttgaca ggcgatgtcg cgcagaaaaa acgtctgcgt attcttgatg
    21361 aatttacccg tggcgatctg gatattctgg ttgccaccga cgttgccgcg cgtggtttgc
    21421 atattccggc agtgacgcac gtctttaact acgatttacc cgatgactgt gaagattacg
    21481 ttcaccgtat tggtcgtaca ggtcgcgcag gcgcaagcgg tcactctatt agcctggcgt
    21541 gtgaagagta tgcattgaat ttgcctgcta ttgagaccta tattggtcac tcaattccgg
    21601 taagcaaata caatccggac gcattgatga ccgatctgcc aaaaccgctg cgcctcacgc
    21661 gcccgcgcac aggcaatggt ccgcgtcgta ctggcgctcc gcgtaatcgt cgtcgttcag
    21721 gttaaataaa aaatgtcata ctcaaaatca ttcaaactgt atcaagatgg caagagaatg
    21781 aatcccgatg agcttactgt agtaagtgat tcaggtgaaa gaatgcagcc aacacagaga
    21841 cagattgaag gatgaagagt atgggttcca cctcatcgct gtatgcagcc attgatctcg
    21901 gttcgaatag ttttcatatg ctggttgtgc gcgaggtggc tggaagcatc cagacgctga
    21961 cgcgaataaa acgcaaagtg cgtctggctg ctggcctgaa tagcgaaaat gccctctcca
    22021 atgaagcaat ggagcgcggt tggcaatgtt tgcgcctgtt tgctgaacgt ctgcaagata
    22081 ttcctccctc gcaaattcgc gttgtcgcta cggcgacgtt acgccttgcc gtcaatgcgg
    22141 gtgattttat tgccaaagcg caggaaatcc tcggttgtcc ggtacaggtg atcagcggtg
    22201 aagaggaagc acgtctgatt tatcagggtg ttgctcacac cactggcggt gccgatcagc
    22261 gcctggtagt ggatatcggc ggtgccagta ctgaactggt aaccggcacg ggtgcacaaa
    22321 ccacctcgtt gttcagcctg tcgatgggct gcgtcacctg gctggaacgc tattttgccg
    22381 atcgtaatct ggggcaggaa aattttgatg ctgcagaaaa agcggcacgc gaagtgttac
    22441 gtccggttgc cgatgaatta cggtatcacg gctggaaagt gtgcgttggt gcttccggca
    22501 ccgtgcaggc gttacaggaa atcatgatgg cacaggggat ggatgaacgc attaccctgg
    22561 aaaagttgca gcaattgaaa cagcgagcca ttcattgcgg tcggctggag gaactggaga
    22621 ttgacgggct gacgctggaa cgtgcgttag tgttcccgag tggtctggcg atcctgatcg
    22681 ccatttttac cgaactgaat attcagtgta tgaccctggc gggcggtgcg ctgcgtgaag
    22741 gcctggtcta cggcatgttg catctggccg tcgagcagga tattcgcagc cgtacgctgc
    22801 gtaatattca acgccgcttt atgatcgata ttgatcaggc acagcgcgta gccaaagttg
    22861 cggctaactt cttcgatcag gtggaaaacg aatggcatct tgaagcaata agccgcgatt
    22921 tgctcatcag cgcctgtcag cttcatgaaa tcggcctgag cgttgacttc aaacaagcgc
    22981 cgcaacacgc tgcttatctg gtgcgtaatc tggatcttcc cggttttacc cccgcacaga
    23041 aaaaattgct ggcgacgcta ctgctcaacc agactaatcc ggtcgatctc tcatcgctgc
    23101 atcagcaaaa tgccgtaccg ccgcgcgtcg cagaacaact ctgccgttta ctacgcctgg
    23161 ccatcatttt tgccagccgt cgccgtgacg atctcgtgcc agagatgaca ttacaggcta
    23221 accatgaact gttgaccttg acgcttccgc aaggttggct aacccaacat ccgctgggta
    23281 aagagattat tgctcaggaa agccagtggc agagctatgt ccactggccg ctggaagtgc
    23341 attaatgagt aagtgccgga tgcgatgctg acgcatcttt tccggccttg attatttccc
    23401 tcgttttgcc gccatcatcg ctttcagatt cgccagatgg ctttgccctt tctgcatccg
    23461 ttcttcggcg ctgaccactt tgcgctcctg ttcccaaatc agatcatcct gcggcagctc
    23521 cagcaaaaag cggctcggct ccgggcgcac cagttcgccg tactgacggc gttctttaca
    23581 cagcgtaaag gtcaattcct tctgggcgcg ggtaatgccg acataggcca gccgccgctc
    23641 ctcatcgata ttatcttcat cgatactgct ctggtgcggc aaaaaccctt cttccatacc
    23701 gaccatgtag acataaggaa actccagtcc ttttgacgcg tggagagtca tcagttgcac
    23761 ctgatccagc tcttcttcac tctcaccacg ctccatcatg tcgcgcaaag taaagcgcgt
    23821 caccacctgg gtgagcgtca tcggctcatc cagttcgctg ccttccagca tctccgtcat
    23881 ccagctaaac agttggttga cgttcttcat gcgcatttcg gcggctttcg ggctgggcga
    23941 tgtttcgtac agccaggatt cataatccat gccatggatc agatcacgca ccgcagcaat
    24001 cggctcccgc tccgccaggc gctggatttc tgccaaccag tgagtgaagc gggtcaatgc
    24061 ttcataacca cgtccgctaa gcgtctgact caggcccata tcaaagctgg cggtaaacat
    24121 gcttttattg cgcgtcatcg cccactcacc cagctttttc agcgtagccg ggccaatctc
    24181 tcgcttcggc gtgttaacga tacgcagaaa tgcgctgtca tcgtccgggt tagtcagcac
    24241 gcgcagataa gccagcaagt ccttgatttc aggacgagag aaaaacgacg taccaccgga
    24301 tattttgtac gggatgcggt tttgcatcag gaatttttca aatacccgcg actgatggtt
    24361 gccgcgataa agaatggcgt aatctttgta ctgcgtttta ttgacgaagt gatgggcgat
    24421 cagctcgcca gtaacgcgct cggcttcatg ttcctcgtta ttcgcgctta aaacttttag
    24481 ctctgtgccg taacccagtt cggagaacag acgcttttca aagacgtgcg ggttattggc
    24541 gatcaggatg ttcgccgctt tcagaatacg cccggaagag cgatagttct gctcaagctt
    24601 aatcaccttc agcgccggaa aatcctgact cagcagcacc aggttttgcg gacgtgcacc
    24661 gcgccaggag tagatcgact ggtcatcgtc acccaccacg gtaaagcgcg cgcggctgcc
    24721 caccagcagt ttcaccagtt catactggct ggtgttggtg tcctgatact catccaccag
    24781 caggtagcga attttgttct gccagcgttc gcgtacttct tcattgcgtt gcagcagcaa
    24841 cgtcggcaat aaaatcaggt catcgaagtc gagaacgtta caggctttca ggtgtgcatc
    24901 atacagccca taacaatggg caaaaatacg atcccgctcg ccctttgcct ctgccgccgc
    24961 ctgcgccgga gttttgagat cgttcttcca gttagagatg gtcgaaatca gttgttgcag
    25021 gagaactttg tcatcttcaa tcagcccctc ggtcaactct ttaagcaaag caagctgatc
    25081 ggtatcgtca aataacgaga agttcgcttt catcccaagc gccgcatact cgcgtttgat
    25141 gatatccagc cccaacgtat ggaaagtgga gatcatcagc ccacgcgcct ctttacgccc
    25201 cagcgtctgc cctacacgct ctttcatctc gcgcgctgct ttattagtaa aggtcaccgc
    25261 cgcaatgtgc cgcgcctgat aaccgcaacc gcggatcaga tgggcgattt tattggtgat
    25321 aacacgagtt ttaccggaac ccgcgcccgc cagcaccagg caggggccgg taacgaattc
    25381 gacagcttgt tgttggccgg ggtttagacg cataggtgta ttgctcaatc ttcgaacggg
    25441 gggaggattg tagcagaatc gcatttgaat gtgaccgcgt gtaagccgct aaccggttgg
    25501 ctgcggatgt ttaaatcaga gagagaagac cgaaatcgga gttcatcgtg aatccattag
    25561 acttagaaaa tatcgggtcg taacgttgtg ttattacttt ttcagagttt actaaggaga
    25621 ggtttactcg tggcgattaa tttcagccct aaggttggtg aaatactgga atgcaacttt
    25681 ggtaactatc ctgttagcca aaatggaccg ttcagcacga cgtattacga tggccgcatt
    25741 cctcctgaaa tgataaaaaa caggttagtt gtcgtgctca acggaaaaat taatggcaac
    25801 gcattcatcg tcgttccatt atcaaccact cgcgaccatg acaagttaaa acgtggaatg
    25861 catgttgaaa tagccagtaa tgttatcaat gacctgcaat tctttgatca gcagattcgt
    25921 tgggccaaaa ccgacctggt acaacaagtc agtcgaaaca gattaaacag agcaagaact
    25981 tacagagggt atcttaatca atgtttgcca catgagttgg ttgcagacat acagagggcg
    26041 gtcatcaagt caatcaatgc catatcgctc ataaattgac aataaaatca ccatccccga
    26101 taatgacttt gtagcccata gaggccctaa gttttctccc cttgagggac ctgcgaagcc
    26161 cggattttcc gggcttttgc catatatgta ccagcccctc cctgactgac aattacgtta
    26221 gcatcggcgg atcatgctaa aatcgccgcc tgacaattat tcactcaagg cacgatcatg
    26281 gcaaaaacag cagcagcact gcatatcctt gtaaaagaag agaaactggc tctggatctt
    26341 ctcgagcaga ttaagaacgg ggccgatttt ggcaagctgg cgaagaaaca ctccatttgc
    26401 ccatcaggca aacgcggcgg tgatttaggt gaattccgcc agggtcagat ggttccggcg
    26461 ttcgataaag ttgtgttctc ttgtccggta ctggagccaa ccggcccgct gcacacccag
    26521 ttcggttacc acatcattaa ggtgctgtac cgtaactaat agcaaggcct tctccgggag
    26581 aaggccttga actctgaatc gctattaacc cgcaacagca atacgtttca tatcagtcat
    26641 gtagccgcgc agtttcttac ctacctgctc aatcgcatgg ctgcgaatcg cttcgttcaa
    26701 atcacgcagt tgcgcgttat ctaccgcacc ttccggaata gctttaccca aatcgcccgg
    26761 ttgcagctct gccataaacg gtttcagcaa cggcacacaa gcgtaagaga acagatagtt
    26821 accgtactca gcggtatcag agataaccac gttcatttcg tacagacgct tacgggcgat
    26881 ggtgttggca atcagcggca gttcgtgcag tgattcatag taagcagatt cttcgatgat
    26941 gccggaatca accatggttt caaacgccag ctcaacgccc gctttcacca tcgcgatcat
    27001 cagtacgcct ttatcgaagt actcctgctc gccgattttg ccttcatact gcggcgcggt
    27061 ttcaaacgcg gttttgccgg tctcttcacg ccaggtcagc agtttcttat catcgttggc
    27121 ccagtccgcc atcatgccgg aggagaattc gccggagatg atgtcgtcca tatgtttctg
    27181 gaacagcggt gccatgatct ctttcagctg ttctgacaac gcgtaagcgc gcagtttcgc
    27241 cgggttagag agacggtcca tcatcagggt gatgccgccc tgtttcagcg cttcggtgat
    27301 ggtttcccag ccgaactgaa tcagtttttc tgcgtatgcc ggatcggtgc cttcttccac
    27361 cagcttgtcg aagcacagca gagagccggc ctgcaacata ccgcacagaa tggtttgctc
    27421 gcccatcagg tcagatttca cttccgcaac gaaagaggat tccagcacgc ccgcacgatg
    27481 accaccggtt gccgccgccc atgctttagc gatcgccatg ccttcgcctt tcggatcgtt
    27541 ttccgggtga acggcaatca gcgtcggtac gccgaatcca cgtttgtact cttcacgtac
    27601 ttcggtgcct gggcatttcg gcgcaaccat tactacggtg atgtctttac ggatctgctc
    27661 gcccacttcg acgatgttga aaccatgcga gtaacccagc gccgcgccgt ctttcatcag
    27721 tggctgtacg gtgtgcacta catcagagtg ctgcttgtcc ggcgtcaggt taaccaccag
    27781 atccgcctgt gggatcagtt cttcgtaagt acccacttta aaaccatttt cggtcgcttt
    27841 acgccaggac gcgcgcttct cggcaatcgc ttctttacgc agagcgtagg agatatcgag
    27901 accagaatca cgcatgttca ggccctggtt cagaccctgt gcgccacagc cgacgatgac
    27961 cactttttta ccctgaaggt agctcgcgcc atcggcgaat tcgtcgcggc ccataaagcg
    28021 acatttgccc aactgtgcca gttgctggcg caggttcagt gtattgaagt agttagccat
    28081 ggtgattcct cgtgatgttg tgcttcttat tgttcggttc gcgtttgcaa gattgaattc
    28141 actatatgac aggaaattta ttgcggaaat tgatatattc acaacatcat attgcaattt
    28201 ttgcaacgtc aacatcgagg gctgtccctg tggatttacg cgatctgaaa accttcctgc
    28261 atctggcgga aagccgccat tttggccgca gcgcgcgggc gatgcacgtt agcccatcca
    28321 cgctctcacg gcagattcag cgcctggaag aagatctcgg tcagccgctg tttgtgcgcg
    28381 ataaccgcac ggtgacgctg actgaagcgg gcgaagaact gcgcgttttc gcccagcaaa
    28441 cgctgttgca gtatcagcag ctgcgccaca ccatcgatca gcaagggccg tcgctctctg
    28501 gcgaattaca tatcttctgc tcggtgaccg ctgcctacag ccatctgccg ccgattctgg
    28561 atcgcttccg cgcggaacac ccgtcggtgg agattaaact cactactggt gatgcggcag
    28621 atgcaatgga aaaagtggtc acgggagaag cggatctggc gattgccggg aaaccggaga
    28681 cgctacccgg cgctgtagcg ttttcgatgc tggagaatct ggcggtagtg ctgattgcgc
    28741 ccgcgctgcc ctgcccggtg cgtaatcagg tctcggtaga gaagccggac tggtcaacgg
    28801 taccgtttat catggccgat caggggccag tacgccgccg cattgaactg tggtttcgac
    28861 gcaataaaat cagcaaccca atgatttacg ccacggttgg cgggcatgaa gcgatggtat
    28921 cgatggtggc gctcggctgt ggtgtggcgc tgttgccgga agtggtgctg gaaaacagcc
    28981 cggagcctgt acgcaaccgg gtaatgattt tagatcgcag cgatgagaaa acgccgtttg
    29041 aactgggcgt gtgtgcacaa aaaaagcggc tgcatgagcc gctgattgag gcgttctgga
    29101 ggattttgcc gaaccacaaa tgacgttgtc gcgcgagtag gcctgataag cgaagcgcta
    29161 tcaggcattt ttccctaacc cgccaaaaag aacctgaacg ccgggttatt ggtttcgtcg
    29221 tggcaatcgt agcccagctc attcagccgg gtttcgaaat ccggttcatg gtcgccaagt
    29281 tcgaacgccg ccagtacgcg cccgtagtcg gtgccatggc tgcgatagtg gaacaaagaa
    29341 atgttccagt gcgtacccag cgtgttgagg aagcgcagca gcgcgcccgg tgattccggg
    29401 aattcgaagc tgtagaggcg ttcctgcaac ggatgtgacg gacgcccgcc gaccatatag
    29461 cgcacatgca acttcgccat ttcgtcgtcg gagagatcca ccacgctgta gccgccgtcg
    29521 ttgagcatct gcaaaatttc tttgcgctct tcgaggccgc ggctcaggcg cacgccgaca
    29581 aagatgcagg cgtttttggc atcggcaaaa cggtagttga actcggtgac cgatcgcccg
    29641 ccaagcagtt ggcagaattt gaggaagctg cctttttctt ccggaatggt caccgccaac
    29701 aacgcttcac gctgttcgcc cagttcgcag cgttccgaga cgtagcgcag gccgtggaag
    29761 ttcacgttgg caccggaaag aatatgcgcc agccgttcgc cgcgaatgtt gtgctgggcg
    29821 atatattttt tcattcccgc cagcgccagc gcgccagagg gttccgccac cgcgcgcaca
    29881 tcttcgaata aatccttcat cgccgcacag atcgcatcac tatcgacggt gatgatatcg
    29941 tcgagatact cctggcacaa acggaaggtt tcgtcgccga tgcgttttac cgcaacgcct
    30001 tcagcaaaca gccccacgcg cggcagatca accggatgac ccgcatccag cgctgctttc
    30061 aggcaggcgg agtcttccgc ttctacggcg attactttga tttgcggcat cagttgtttg
    30121 atcagcaccg ccacgccagc agccagaccg ccgccgccga ctggcacaaa tacgcggtcg
    30181 agatgggcgt cctgctggag cagttccagc gccagcgtgc cttgcccggc aatcaccatc
    30241 ggatggtcga acggcggcac ccaggtgaac ccctgctgct gtgacagttc gatcgctttg
    30301 gctttcgctt catcaaagtt cgcgccgtgg agcagcactt cgccgccgaa gccgcgcacc
    30361 gcgtcgactt tgatgtcggc ggtggcggtt ggcataacga tcagggcctt cacgcctaac
    30421 cgcgcagaag aaaacgcgac gccctgcgcg tggttacccg cagaagcagt gatcacgccg
    30481 tgcgcttttt gttcttccgt caggcccgcc atcatggcgt atgcgccgcg cagcttaaag
    30541 ctgtgcactg gctggcgatc ttcgcgcttc accagaatca cgttatcaag acgagacgag
    30601 agcttttcca ttttttgtag cggcgtgacc tgcgccgctt cgtagaccgg cgcgcgcagc
    30661 accgctctta aatattcagc accttccggg gcaccggaca ggggttgcga gtcagccatt
    30721 attaaccccc cagtttcgat ttatcgcgca ccgcgccttt gtcggcgctg gttgccaggc
    30781 tggcataagc acgcagggca aaggagatct gacgttcacg atttttcggc gtccaggctt
    30841 tgtcaccccg agcttcctgc gcttcacgac gcgccgccag ttcggcatcg cttacctgta
    30901 actgaatgcc acggttcgga atgtcgatgg cgatcaggtc gccgtcttca atcaggccaa
    30961 tgctgccgcc gcttgccgct tccggtgaga cgtggccgat agaaaggcca gaggtgccgc
    31021 cagagaaacg accgtcggtg atcagcgcac aggctttacc gagccccatt gatttcagga
    31081 agctggttgg gtagagcatt tcctgcatcc ccggaccacc ttttgggcct tcatagcgaa
    31141 ttactaccac atctccggcg acaactttac cgccgagaat cgcttctacc gcgtcgtcct
    31201 ggctttcgta cactttcgcc gggccggtga atttgaggat gctgtcatcg acgcccgcag
    31261 ttttaacgat gcagccgttt tccgcgaaat taccgtagag caccgccagg ccgccgtctt
    31321 tgctataggc gtgttccagc gagcggatac agccattggc gcgatcgtcg tccagcgtat
    31381 cccaacggca atcttgcgag aatgcctggg tggtacggat acccgcaggg ccggcgcgga
    31441 acatattttt taccgcgtca tcctgggtca gcataacgtc gtattgttcc agcgtttgcg
    31501 gcaacgtcag gccaagtacg tttttcacat cacggttcag taaccccgca cgatccagct
    31561 cgccgagaat accgataaca ccaccagcac ggtgaacatc ttccatatgg tatttttggg
    31621 tgctcggcgc gactttgcat aactgcggaa ccttgcggga aagcttatcg atatcactca
    31681 tggtgaagtc gatttccgct tcctgcgccg ccgccagcag gtgaagtacg gtgttagtcg
    31741 atccacccat cgcgatatcc agcgtcatgg cattttcaaa cgccgctttg ctagcaatat
    31801 tgcggggcag tgcactttcg tcgttttgct cgtagtaacg tttggtcaat tcaacaatgc
    31861 gtttaccagc attgaggaac agctgcttac ggtcagagtg ggttgccagc agcgagccgt
    31921 tgcccggctg cgacagaccc agcgcttcgg tcaggcagtt cattgagtta gcggtaaaca
    31981 tcccggagca ggaaccgcag gttgggcatg cggaacgttc aacctgatcg ctctgggagt
    32041 cagatacttt cgggtctgcg ccctggatca tcgcatcaac cagatcgagc ttgatgatct
    32101 gatcggacag tttggttttc ccggcctcca tcgggccgcc ggaaacaaag atcaccggaa
    32161 tattcagacg cagggaagcc atcagcatcc ccggggtgat tttgtcacag ttagagatgc
    32221 agaccatggc gtcggcgcag tgggcgttga ccatatactc aacggaatca gcgatcagtt
    32281 cgcgagatgg cagtgaataa agcatccccc cgtggcccat ggcaatccca tcatccaccg
    32341 caatggtgtt gaactctttg gcaacgccgc cagccgcttc aatttgttcg gcgaccagtt
    32401 taccgagatc gcgcagatgg acgtgacccg gtacaaattg ggtgaacgag ttcacaaccg
    32461 cgataatcgg cttaccgaaa tcggcgtcgg tcattccggt ggcgcgccac agcgcacgag
    32521 cacccgccat attacgacca tgagtggtgg tggcggaacg gtacttaggc atactttatt
    32581 tactcccagt gtctgtctcg taaatgggac ggtgcgtgcc gtcccatttt ttgtatttat
    32641 tgattaactt gatctaacca gccccattta tcttcggttt cgccggtaaa gaggccgaag
    32701 aaggcttgct gaatgcgttt ggtgaccggg ccacaacggc cttcgccaac ctgaataccg
    32761 tctacactgc gcactggcgt gatttctgcc gccgtaccgg acataaagac ttcatccgcc
    32821 aggtacaagg attcgcgcga cagcacctgc tcacgcactt caattcccag ctctttcgcc
    32881 agtttgatga tggcatcacg ggtaataccc ggcagcgcgg aggaggtgaa cggtggggtg
    32941 aacagcacac catctttcac ttcaaacagg ttttcgcctg cgccttcaga gatataaccg
    33001 ttcacatcca gcgcgatacc ttcctgataa ccgtggcggc gcgcttcgct acccaccagc
    33061 agggaagaga gatagttacc accggctttt gccgcagttg ggatggtgtt tggtgctgcg
    33121 cggttccagg aggaaaccat cgcatcgatc ccctgctcca gcgcttctgc gcccagatac
    33181 gctccccacg ggaaagcggc gataatcaca tcggttgagt atcccgctgg cgggtttacg
    33241 cccatgccaa catcaccaac gaagatcagc ggacggatat aggcgctggt gagattgttt
    33301 ttgcggatca cgtcacggca agcttccatc agctcatcaa tgctctgcga aaccgggaag
    33361 cgatagattt tggcggagtc atgcagacgc tgcatatgct cacgatggcg gaatacaacc
    33421 ggtcctttgt gcgagtcgta gcaacggatg ccttcaaaaa ccgaggtgcc atagtgcagc
    33481 gcgtgcgaca tcacatgcac cttcgcgtct tcccagcgaa ccatctcccc attgaaccaa
    33541 atgtaatcag ctttcttcgt ggtcattttt atattccttt tgcgctcagg cgcggatttg
    33601 ttgtgatgtg gttgtgctct ggcagatggc aacgtgtgcg acgtccacca gtttatttaa
    33661 ctgactaaac agtaagtcga ccgaccgtgg gctggcaacg gtcaattcga tatttatatt
    33721 ttgtgcatcg ctggcggcgg ccatattcat tgagcagacg tggaaaccac gatgacgcac
    33781 cacgcgtaaa acacgttcta aggtttccgg attgaagcga gccgatacat tgacctgatg
    33841 ttgcatcatg ataatttctc caacatttct gaattactgg caccaggcgg caccagcggc
    33901 cagacgttct caagttcgtc gattgagaca tgaagcaggt atggcccatc actgttcagc
    33961 atggtgtcga gtgccgcttc aacctggtct ttacgggtga tgtgttggcc agggatgccg
    34021 aaggcgctgg ctaacttgag gaaatcgggg ttatcggtaa gggtggtttc gctgtatcgt
    34081 tcctggaaaa acagttgctg ccattgtcga accatcccta accgttggtt atcgagtaag
    34141 acgattttca acggtaactg cttgcgtttt acggtgccca gctcttgtac attcatcatg
    34201 aaagagccgt caccggagat acagacgacg gtatcgtttg gtcgcgcgac ttgcgcgcca
    34261 accgccgccg gtaaaccaaa acccatggtg cctaagccgc tggaggtaat gaaattttcc
    34321 gggcgagtgt gtgcgatgtg ctgcgcggcc cacatctgat gctgccccac atctgtggtc
    34381 acaacgcaat ccgcaggttt acgatccgac agttgtttta acaacaacgg cgcgtagata
    34441 gcgtcaccgg gatggtcgta acgccaggca tgttcatcac gcagctgcgc gcagtattgc
    34501 tgccagtcat tgatatttaa tggctgctgt aatgctggta acagcgcatt taaatcaccc
    34561 tgtaatgcca catgtgcctg acgcagcttg ttcatttctg ccgggtcgat atccatatgg
    34621 ataacactgg cgtgtggtgc gaaggtgttc aatttgccgg tcacccggtc atcaaaacgt
    34681 gcacccacgg cgatcagcaa gtcgcactcc tgcaccgcga agttcgccgc tttggtgcca
    34741 tgcattccca gcatgcccag atagtacgga taatctgctt caactgcgcc cagccctttc
    34801 agcgtgcagg tggcaggcat ttttgtggta gcgagaaatt ctcgtaaagc aggaactgcc
    34861 tgcgccatac ccacgccacc accaacgtac agcatcggtt tttgcgcttt tgccagcatc
    34921 tggcgcgctt gctcaacttc ggcatgtggg aaagtcactt cgttttcaac ggtggtgaac
    34981 cacggttcca ggtcgccgct ggctaactgg atatcttttg ggatatcgac cagaaccgga
    35041 ccaggacgac ctgagctggc aacgtcgaat gcttcagcca taatgcgcgg caactcttcc
    35101 agcgactgca ccagaaagct gtgcttggta caggctaacg acaatcccag gacatccact
    35161 tcctgaaatg cgtcagtacc gataaacggt gcggacactt gaccggtgat ggcaacaaca
    35221 gggatagaat ctaacagtgc gtccgcaagc ccggttatca ggttggttgc gcccggacca
    35281 gacgtggcga tacatacgcc agttttgccg gtagcacggg cataaccgat agccgccatt
    35341 gccgcaccct gctcatgtcg gcacagcaag tgctccacgc cgccgtcata caatgcatcg
    35401 taaaccggca taattgcgcc acccggataa ccgaaaacgg tgttcacacc ctgtgcccgc
    35461 aacgcatgta ccacccactg tgcgccattc atagttagtt ccccgtcctg aatcttggga
    35521 aacagaattt tgtgctgtta ttcattgtct gctcctcggt tatgttttta aggtcaaaaa
    35581 aaaaccccgg acctttcggt gcgggggtct tagttcgtta aggcttgatc tctaagcctt
    35641 tcctcgtcca agtgcagccc cgcacggtgg gataataatc accaccacgc taatcacgac
    35701 caggctaatc actcgtagaa gggctgtcat tttgtctttt cttgcatctt gttcgaagga
    35761 atgcctaaag agttaccata gattttgcga atagcacaag atattttttg gccgtttttt
    35821 atgatggctt taataatttc aatagaaatt gttgtttttt aaaaagaaaa tagtgagtga
    35881 aaaaaattca atttcagtac gaatgttgtt ccctccctcg tagttcatcc tttgtccgct
    35941 gcatttttgc gagcatcatt ccaccgccag aacaactcaa cttcacttgc acaaataaac
    36001 aggaatcagc cactaaaatt cacgaattag cgggctacag cagcgatatc ttatgcacat
    36061 aatggtgact caaggagggc ttatgtcact gtcaattgtt catacccgcg cagccctggg
    36121 agtaaatgcg cccccaatca ctgttgaggt acatatcagt aaaggtttgc ccggcttaac
    36181 gatggtgggc ttaccagaaa caacggtaaa agaagctcgc gatcgcgtgc gcagcgccat
    36241 tatcaatagc ggatatgaat atccggcgaa aaaaatcacc attaaccttg ctccagccga
    36301 tctaccaaaa gaagggggac gatatgattt acctatcgcc attgcgttgc tggcggcctc
    36361 agaacagctt acagccaata agttagatga atatgaatta gtcggagaac tggcgcttac
    36421 aggcgctctg cgtggcgttc ccggtgcaat ctccagtgca actgaagcta ttaagtcggg
    36481 cagaaaaatt atcgtcgcga aagataacga agatgaagtg gggctaatta acggtgaagg
    36541 atgcctggta gccgatcatc tgcaagctgt ctgtgcgttt ctggaaggta agcacgctct
    36601 cgaacgcccg aaaccaactg atgcagtatc ccgggcgcta caacatgatc tcagtgatgt
    36661 tgtcggtcag gagcaaggaa agcgaggact ggaaattacc gccgctggcg ggcacaacct
    36721 tttactgatt gggccgccgg gaacaggtaa aacaatgctc gccagccgta ttaatggtct
    36781 tttgccagat ttaagcaatg aagaggcact ggagagcgct gcgatattaa gtctggtaaa
    36841 tgctgaatca gtacaaaaac aatggcggca gcgcccgttc cgctcacctc atcacagtgc
    36901 gtcgttaacg gcgatggtgg gcggtggtgc aattccaggg ccaggtgaaa tttcgttggc
    36961 gcataacggc gtgctttttc ttgatgagct acctgaattt gaacggcgta cactggatgc
    37021 tttgcgagag ccgattgaat ccgggcagat ccatctttca cgcacacgag caaaaataac
    37081 ctatccagcc cgtttccagc ttgttgcggc gatgaatccc agccctaccg gacattatca
    37141 gggaaaccat aaccgctgca cgccagaaca gacattacgt tatctcaacc ggctctcggg
    37201 gccctttctc gaccgcttcg atctctcact ggagatccca ttaccacccc ccggcatttt
    37261 gagtaaaacg gtagtgccgg gagaaagcag caccaccgtt aaacaacgtg taatggctgc
    37321 cagagagcgc caatttaagc ggcagaataa gttgaacgcc tggctggata gtccggaaat
    37381 acgcaaattc tgcaagcttg agagcgaaga tgcgcagtgg ctggaagaaa cgctgatcca
    37441 tctggggtta tcgattcgtg cctggcagcg gttattgaaa gttgcacgaa ccattgctga
    37501 tattgatcag tctgacatta tcacacgtca gcatttgcag gaggcagtta gctatcgtgc
    37561 gattgaccgt ttgctcatcc atctgcaaaa actactgaca taaaaaaagg gcatttcgcc
    37621 ctttttatta atcgtcagaa tcggtgtagt cttcagcacc ttcaacctgc ggtttaccgc
    37681 cggaaagggt gtgaaaacgt tttggacgct tgatacgcgt catatacttg gaccacacgc
    37741 gttctgcttc tgtcactggc tcacgttcgc cacggcatac tgctacgaag agtttctctt
    37801 cctcggtaac cggctcgcgt ttgccaagat ccaactcatt gaaggcataa ccatgacgct
    37861 caagcagttg tgcctctttg atggtgaaat caccatggcg agagaatcca cgtggataat
    37921 gtttattgtc gaaatatcga ttagtcgtcg taaagctttc cgccatcctg cacgctccta
    37981 attctttgac cgagctagtt atggcgcgga gtattagtta cgcttgacag agtgtaaaac
    38041 aaaacattta aatcataacg acaaataatt ttgcggagag cactgtggat acggaattgt
    38101 taaaaacttt cctggaagtt agccgaacgc gtcactttgg tcgagcggct gaatcgctct
    38161 atctgaccca gtcagcagtg agctttcgaa tcagacaact ggaaaatcaa ctgggtgtga
    38221 accttttcac ccgccacaga aacaatatcc gtttaaccgc tgccggtgaa aaactactgc
    38281 cttatgcaga aacgctcatg agcacctggc aggccgcccg taaggaggtg gcgcatacct
    38341 cacgacataa cgagttttct atcggtgcca gcgcctcatt gtgggaatgt atgcttaatc
    38401 agtggctggg acgtttgtat caaaatcagg atgcccatac aggcttacag ttcgaagcgc
    38461 gaattgccca acggcagtct ctggtaaaac agctgcatga acgccagctt gatcttctta
    38521 ttaccactga agcgcccaaa atggacgaat ttagtagtca gttgctgggg tatttcactt
    38581 tagcgcttta taccagtgcc ccttctaaac taaagggaga tcttaactat ctgcgactgg
    38641 agtgggggcc agattttcaa cagcatgagg caggtttgat cggtgctgac gaagtgccca
    38701 ttctgacaac cagttctgct gaactggcac agcaacagat tgcgatgctt aatggttgca
    38761 cctggctacc cgtcagctgg gcgcgtaaaa aaggcggcct gcataccgtt gtcgatagca
    38821 caacactttc acggccgctt tatgccatat ggctgcaaaa tagcgataaa aatacgttga
    38881 ttcgcgatct attgaaaatt aacgtgctgg atgaagtgta ttaatctgaa tggctggcaa
    38941 ggatgccggt agaaggattt acttcagaga gggttatttc agataaaaaa aatccttagc
    39001 tttcgctaag gatgatttct ggcaggggcg gagagactcg aactcccaac acccggtttt
    39061 ggagaccggt gctctaccaa ttgaactacg cccctaatta gggtggcgga acggacggga
    39121 ctcgaacccg cgaccccctg cgtgacaggc aggtattcta accgactgaa ctaccgctcc
    39181 accgaattct tttacaacca ccggttttat gaccggctt
//
LOCUS       DAZKIP010000029        38473 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZKIP010000029 DAZKIP010000000
VERSION     DAZKIP010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.029"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(112..303)
                     /gene="yfgG"
                     /locus_tag="ACHOUK_001780"
     CDS             complement(112..303)
                     /gene="yfgG"
                     /locus_tag="ACHOUK_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFP9897244.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            321..473
                     /locus_tag="ACHOUK_001781"
     CDS             321..473
                     /locus_tag="ACHOUK_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9897245.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKSSVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            655..2898
                     /gene="pdeF"
                     /locus_tag="ACHOUK_001782"
     CDS             655..2898
                     /gene="pdeF"
                     /locus_tag="ACHOUK_001782"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFP9897246.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDVKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRTVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLDKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2937..4478)
                     /gene="ppx"
                     /locus_tag="ACHOUK_001783"
     CDS             complement(2937..4478)
                     /gene="ppx"
                     /locus_tag="ACHOUK_001783"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFP9897247.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(4483..6549)
                     /gene="ppk1"
                     /locus_tag="ACHOUK_001784"
     CDS             complement(4483..6549)
                     /gene="ppk1"
                     /locus_tag="ACHOUK_001784"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFP9897248.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6720..7358)
                     /gene="purN"
                     /locus_tag="ACHOUK_001785"
     CDS             complement(6720..7358)
                     /gene="purN"
                     /locus_tag="ACHOUK_001785"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFP9897249.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHPHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDVTARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(7358..8395)
                     /gene="purM"
                     /locus_tag="ACHOUK_001786"
     CDS             complement(7358..8395)
                     /gene="purM"
                     /locus_tag="ACHOUK_001786"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFP9897250.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVNAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8720..9346
                     /gene="upp"
                     /locus_tag="ACHOUK_001787"
     CDS             8720..9346
                     /gene="upp"
                     /locus_tag="ACHOUK_001787"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFP9897251.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            9432..10721
                     /gene="uraA"
                     /locus_tag="ACHOUK_001788"
     CDS             9432..10721
                     /gene="uraA"
                     /locus_tag="ACHOUK_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFP9897252.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            10816..11517
                     /gene="hda"
                     /locus_tag="ACHOUK_001789"
     CDS             10816..11517
                     /gene="hda"
                     /locus_tag="ACHOUK_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFP9897253.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(11655..12014)
                     /gene="arsC"
                     /locus_tag="ACHOUK_001790"
     CDS             complement(11655..12014)
                     /gene="arsC"
                     /locus_tag="ACHOUK_001790"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFP9897254.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(12035..13498)
                     /gene="bepA"
                     /locus_tag="ACHOUK_001791"
     CDS             complement(12035..13498)
                     /gene="bepA"
                     /locus_tag="ACHOUK_001791"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFP9897255.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRNAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13711..14772
                     /gene="yfgO"
                     /locus_tag="ACHOUK_001792"
     CDS             13711..14772
                     /gene="yfgO"
                     /locus_tag="ACHOUK_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP9897256.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(14810..15658)
                     /gene="focB"
                     /locus_tag="ACHOUK_001793"
     CDS             complement(14810..15658)
                     /gene="focB"
                     /locus_tag="ACHOUK_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFP9897257.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSICYRAI
                     YLRQES"
     gene            complement(15680..17692)
                     /gene="hyfR"
                     /locus_tag="ACHOUK_001794"
     CDS             complement(15680..17692)
                     /gene="hyfR"
                     /locus_tag="ACHOUK_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFP9897258.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLHLLASNGCESSLLIPLTFGN
                     HTPGALLLAHTSTTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRAIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(17722..18135)
                     /gene="hyfJ"
                     /locus_tag="ACHOUK_001795"
     CDS             complement(17722..18135)
                     /gene="hyfJ"
                     /locus_tag="ACHOUK_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFP9897259.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVTFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            complement(18128..18886)
                     /gene="hyfI"
                     /locus_tag="ACHOUK_001796"
     CDS             complement(18128..18886)
                     /gene="hyfI"
                     /locus_tag="ACHOUK_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFP9897260.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKRHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGQEICDRLLRHLSND
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(18883..19428)
                     /gene="hyfH"
                     /locus_tag="ACHOUK_001797"
     CDS             complement(18883..19428)
                     /gene="hyfH"
                     /locus_tag="ACHOUK_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFP9897261.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPTNALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            complement(19438..21153)
                     /gene="hyfG"
                     /locus_tag="ACHOUK_001798"
     CDS             complement(19438..21153)
                     /gene="hyfG"
                     /locus_tag="ACHOUK_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFP9897262.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVQYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYPFINEGNSGARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(21143..22723)
                     /gene="hyfF"
                     /locus_tag="ACHOUK_001799"
     CDS             complement(21143..22723)
                     /gene="hyfF"
                     /locus_tag="ACHOUK_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFP9897263.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGHSATRTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     EHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(22728..23378)
                     /gene="hyfE"
                     /locus_tag="ACHOUK_001800"
     CDS             complement(22728..23378)
                     /gene="hyfE"
                     /locus_tag="ACHOUK_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFP9897264.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(23390..24829)
                     /gene="hyfD"
                     /locus_tag="ACHOUK_001801"
     CDS             complement(23390..24829)
                     /gene="hyfD"
                     /locus_tag="ACHOUK_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HFP9897265.1"
                     /translation="MENLALTTLLLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVCTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWVLISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFALSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            complement(24846..25793)
                     /gene="hyfC"
                     /locus_tag="ACHOUK_001802"
     CDS             complement(24846..25793)
                     /gene="hyfC"
                     /locus_tag="ACHOUK_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFP9897266.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPISSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGCAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(25804..27822)
                     /gene="hyfB"
                     /locus_tag="ACHOUK_001803"
     CDS             complement(25804..27822)
                     /gene="hyfB"
                     /locus_tag="ACHOUK_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HFP9897267.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHEGVTLVARFATPFDFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISALPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTQVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(27822..28439)
                     /gene="hyfA"
                     /locus_tag="ACHOUK_001804"
     CDS             complement(27822..28439)
                     /gene="hyfA"
                     /locus_tag="ACHOUK_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFP9897268.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCVQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENAPTQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGREDPLSLTQEQR"
     gene            complement(28692..29162)
                     /gene="bcp"
                     /locus_tag="ACHOUK_001805"
     CDS             complement(28692..29162)
                     /gene="bcp"
                     /locus_tag="ACHOUK_001805"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFP9897269.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(29162..29734)
                     /gene="gcvR"
                     /locus_tag="ACHOUK_001806"
     CDS             complement(29162..29734)
                     /gene="gcvR"
                     /locus_tag="ACHOUK_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFP9897270.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            29880..30758
                     /gene="dapA"
                     /locus_tag="ACHOUK_001807"
     CDS             29880..30758
                     /gene="dapA"
                     /locus_tag="ACHOUK_001807"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFP9897271.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            30775..31809
                     /gene="bamC"
                     /locus_tag="ACHOUK_001808"
     CDS             30775..31809
                     /gene="bamC"
                     /locus_tag="ACHOUK_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFP9897272.1"
                     /translation="MAYSVHKSRLATVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            32022..32735
                     /gene="purC"
                     /locus_tag="ACHOUK_001809"
     CDS             32022..32735
                     /gene="purC"
                     /locus_tag="ACHOUK_001809"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFP9897273.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            32903..33766
                     /gene="ypfJ"
                     /locus_tag="ACHOUK_001810"
     CDS             32903..33766
                     /gene="ypfJ"
                     /locus_tag="ACHOUK_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFP9897274.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            33781..35796
                     /gene="tmcA"
                     /locus_tag="ACHOUK_001811"
     CDS             33781..35796
                     /gene="tmcA"
                     /locus_tag="ACHOUK_001811"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFP9897275.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHFKRVLTADNDAIFWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQILQQLLTMPSGVAAVTAARGRGKSALAGQLISRIAG
                     SAIITAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGVGQQLIASALQYRPGLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNNAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            35870..36568
                     /gene="ypfH"
                     /locus_tag="ACHOUK_001812"
     CDS             35870..36568
                     /gene="ypfH"
                     /locus_tag="ACHOUK_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFP9897276.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(36649..36849)
                     /gene="ypfN"
                     /locus_tag="ACHOUK_001813"
     CDS             complement(36649..36849)
                     /gene="ypfN"
                     /locus_tag="ACHOUK_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFP9897277.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(36877..38004)
                     /gene="dapE"
                     /locus_tag="ACHOUK_001814"
     CDS             complement(36877..38004)
                     /gene="dapE"
                     /locus_tag="ACHOUK_001814"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFP9897278.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKAVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(38008..38364)
                     /gene="yffB"
                     /locus_tag="ACHOUK_001815"
     CDS             complement(38008..38364)
                     /gene="yffB"
                     /locus_tag="ACHOUK_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFP9897279.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNIITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQKFFHEV"
BASE COUNT         9397 a         9923 c        10112 g         9041 t
ORIGIN      
        1 aagacgtata atttaagatg aaagaatatc tgccgctttg ataattccaa agagcaaaag
       61 catccgacag cgaagcgccg ttatgttctg tgaaagtgga gaaggtaacc gctaacgttg
      121 taccggtgat tcgacggaga gtgtggattg ctgagcttct tttttgctct gatggtgctg
      181 ccaggcaccg acggacgagt agataaaacg gccaaagaag aagataaagc tgatgagcag
      241 tacgatacgg gtcatgcgac tgttaaatcg gtgtcgtttt cgcatactgg ttgcctgact
      301 cacaaaaggt tccttgaagt atgtcccacg ccgtggacgg tacttacatt aaggcacaac
      361 aggacaaaat ggtcaattct tcgttatgta aaaaatcgtc agtggataca tattttaatg
      421 ttatggaagt taatttaatt atttacaatg atgatgtaat aatgatgaat taacatgata
      481 aacgtaataa ttcattaatc agggttattt tgtttgatat atatcaattg gaactttcat
      541 atgacactta gaatcaatgc tctctatagt gatgaataat catcattcga agtcaggtgg
      601 gatgcctgtc tgaatacacc tccttcagga tgtgggggat tctgctgagc atctatgaaa
      661 ctgaatgcaa cttatataaa aatacgtgat aaatggtggg ggcttccgct gttcctgcct
      721 tctttaatct tgcccatttt cgcccacatt aatactttcg cgcatatttc ttccggtgag
      781 gtttttctct tttatctgcc tctggcactg atgatcagca tgatgatgtt tttcagctgg
      841 gcggcattgc cagggatcgc cttagggatt tttgtccgca aatatgcaga gctgggtttt
      901 tacgaaacgc tctcattaac ggctaatttt attatcatta tcattctctg ttggggcggt
      961 tacagggtct ttactccccg gcgtaacaac gtttcacatg gtgatacccg tttaatttcc
     1021 cagcgtatat tctggcagat tgtgtttcct gcaacgctgt ttctgatact tttccagttt
     1081 gctgcatttg taggattact ggcgagcaga gaaaatctgg ttggcgtcat gccttttaat
     1141 ctcgggacct taatcaatta tcaggcctta ctggtgggca atctgattgg tgtcccgctg
     1201 tgctacttca tcattcgggt agtgcgaaat ccgttttatt tacgtagtta ttattcgcaa
     1261 ttaaaacagc aggttgatgt caaagtcacc aaaaaagagt tcgcactctg gctgctggca
     1321 ttaggtgctt tactgttgct gttatgcatg ccgttaaatg aaaaaagcac aatttttagc
     1381 accaattata ccttgtcatt attgctgccc ctgatgatgt ggggagcgat gcgctatggt
     1441 tataagctga tttcgctgct ctgggcggtc gtgttgatga tcagcatcca cagctatcaa
     1501 aattacattc ccatttatcc tggctatacc acgcagctga ccataacctc ctccagttat
     1561 ctggtattct cttttattgt caattatatg gctgtactgg caacccgtca gagaacggta
     1621 gtcagacgca ttcagcgcct tgcgtatgtg gacccggtgg ttcatctgcc aaatgttcgc
     1681 gccctgaatc gcgcgttgcg tgatgccccc tggtctgcgc tttgttattt acgcatccct
     1741 ggcatggaaa tgctggttaa aaactatggc atcatgctgc ggattcaata caagcaaaaa
     1801 ctttctcact ggctgtcacc cttgctggaa ccaggtgaag atgtttatca gctttcgggt
     1861 aacgatctcg cgctgcgact gaatacagaa tcgcaccagg agcgcattac cgcactggat
     1921 agccatctca agcaatttcg tttcttttgg gatggcatgc cgatgcaacc gcagattggc
     1981 gtcagttact gctatgtgcg ctcgccagtg aatcatatct acctgctgct gggagagcta
     2041 aatacggtcg ccgaactttc catcgtgacc aacgccccgg aaaatatgca gcgtcgcggg
     2101 gcaatgtatt tgcaacgcga attgaaagat aaagtcgcga tgatgaatcg actacagcag
     2161 gcgctggaac acaaccattt tttcctgatg gcccagccga ttaccggtat gcgtggtgat
     2221 gtttaccatg aaattcttct gcgcatgaaa ggtgagaatg atgaactaat cggccccgat
     2281 agcttcttgc cggtcgcgca cgaatttggt ttatcgtcga gtatcgacat gtgggtcatt
     2341 gagcatacgc tgcaatttat ggctgaaaac agagcgaaga tgcccgctca ccgttttgct
     2401 attaatctgt ctccaacctc ggtatgtcag gctcgttttc ctgttgaagt cagtcagttg
     2461 ctggataaat atcagattga agcgtggcaa cttatttttg aagtcaccga aagtaatgct
     2521 ctgaccaatg ttaagcaggc gcaaatcacc ttgcagcatc ttcaggaatt aggctgccag
     2581 attgcgattg atgatttcgg caccggctac gccagctatg cgcggcttaa aaatgtgaat
     2641 gccgatctgc ttaaaattga cggcagtttt atccgcaata ttgtgtcaaa tagtctggat
     2701 tatcagatag tggcatcgat ttgccacctg gcgcgaatga agaaaatgcg ggtagtggca
     2761 gagtacgttg aaaacgaaga gatccgcgag gcggtgctct ctttggggat cgattatatg
     2821 cagggttatc ttattggtaa gccgcaaccg ttaattgata cgctgaatga aatcgaaccc
     2881 attcgcgaaa gtgcctgaat aatgcgggcc gacatttctc gtcggcccgc aaagcattaa
     2941 gctgcgattt caggtgtact ttcttcttca attttcaacc gccagccagc cacgccttcc
     3001 cagtattctt gctccttttc cagatcaagc agtaccagcg cattctgact aaaccagtca
     3061 tgcgggaaac gcagtgtcca gtgactgtca tcagtaatca gcgtcaatgt tggcggtgtg
     3121 gtggttgcct gacgctgatt gttgagtaat acgccaaggc gcaatagctg aatcagtggc
     3181 aggaactgtt tcttcttaaa caaggtaaag cgcggcagat cgtcgagttt aatcgcttta
     3241 cggtgatagc gcaccagtgt cgccatcatc agctgctgtt cctgattaaa acccggcaag
     3301 tcgctgtttt gtagaatata agcggagtga cgatgcaaac cgctgtggtt gatattcaac
     3361 ccgacctcat gcagcatggc ggcccatcgc agtagcgcct ccagttgcgg atgcgccagc
     3421 ttcggttgct gttcccgcca ctgttcgtac atttgcatag tggtatccag cacccgtcgg
     3481 gcctgttcgc tgtcgatgtg atactggttg gcgaggctgc tggcggtgcg actacgcaca
     3541 tcctgatgac ggaaacgtcc ttccatttca tacagtacgc cttcgcgtaa cgccccgtca
     3601 gaaaggcgca gttcacggat ggctaaagca tcaaacacac cgcataaaat cgctagtcca
     3661 ggaacgaaga ctgttttccg ctcttcggaa agacctggta aactcagcga tgcgaaatta
     3721 cggtgccgta aaacttcttt taccagtttt tccagacgtt ccggggtaat tatcccgtct
     3781 ttctcgccca tttccattaa cacttcatgg gcggctttta tggtaccgga agcgcccatt
     3841 gccacgttcc agccctgaat acgaaattgc caggttaaag tttccagttt ttgtgctgcc
     3901 gccatgcgag cgcgctgaaa attctcttta ttgatgaccc cgccagggaa ataaagctgg
     3961 gcaaagctga cacaccccat ccggcggctt tcaacgagaa tcggttcgaa attttcacca
     4021 atcaccagtt ctgtagaacc gccgccaata tcaataacca gtttgcgacc tttttccggt
     4081 tgggtatgtt ccacgcccat aaaaatcaga cgggcttctt cattaccgga aataatttca
     4141 atcgggtagg gaatgacctt ttccgcacgt ttcagaaagt cagtggcgtt cagcgcctga
     4201 cgcagcgtat gggtaccaac tatacagacg ctggcaggag aaaacccttg tagccgttcg
     4261 gcaaacagcg acagacagtt caaaccgcgc gtcattgcct cttcactcaa catattatct
     4321 ggccccaggc cgtccgccag atgcacccgc tgtttcaggc ggccaataat ctgcatggca
     4381 ccatctacca cacgggctat gaccatgtga aaactgtttg aaccaagatc gaccgcagca
     4441 aactcctgcg gacgagggga tttatcgtgt attggcatag ggttattcag gttgttcgag
     4501 tgatttgatg tagtcataaa tcgccaactg cgcccgtact ttgcggcgat tgccgcgggg
     4561 aacgtagcga ttactgagtt ctttatcgat ataacgtgct ttgaccgtat cgctgaacaa
     4621 tatgtcgatg atgtccagca cccgctgctt caggcgcgga tcgagcagcg gcgtcgccac
     4681 ttcaatacga taatcaatat tgcgcgtcat ccagtcggcg gaagaaaggt agaccttttt
     4741 atcgccgcca ttttcaaaaa tataaacccg gtcatgttca aggtaacggt caacaatact
     4801 gatggcacga atgttgtcgc taatgccttc cagattgggg atcagcgaac acattccgcg
     4861 aaccagcaga ttaaccggta cgccggagct ggaggccgca tacagacgat caaccaggcc
     4921 tttatcgaca aggttattta gcttcagggt gataccactg ggcagccctt gctgcgcgtt
     4981 ggcgatctcg cggtccacca tttcatacaa caggcggcgg gagttttgcg gcgacaccat
     5041 taaataatca aatgtcaccg gacggtatgg gttttcaata aagttaaata cccgccgtac
     5101 ttcgttggtg atgcgcgcat cggcggtcag caacgaatag tcagtataaa gacgcgcggt
     5161 tttttcgtta aagttcccgg tcccgatgtg tgcataacgc accacttcac cgttttcttt
     5221 acgtgaaatc agaaacagtt tggcgtgaat tttcagcccc ggcgcagaga agataacgtg
     5281 cacgcctgct tcggtcaggc gcttcgccca gtgaatgttg gcttcttcgt caaaacgtgc
     5341 ctgtagctca accaccacgg tgactttctt accgttgtgt gcggcgtgga tcatcgagtc
     5401 gatgatgcgt gaatcttttg ccacacggta aatgttgatt ttaatcgcca gcacgctcgg
     5461 atcgaacgag gcctgacgca gcagttccag cacatgctca aaggtgtgat aaggataata
     5521 gagcaacaca tcgcgttcgc gaatggcatc aaaaccattg cggaactggg ctttatcaaa
     5581 ccaaatatgg cgtaaacgcg gcagtggttt gttcaccaga ttggctttgc cgacattggg
     5641 gaaattaata aagtctttaa aattatgata acgaccgccg gggacgatgg agtcgtagcg
     5701 ggaaatagtc agtttttcgc gtaacacttc aaccagcgca ttgggcatat cgcgctgata
     5761 aacaaaacgc accggctcag cagttaaacg ctgcttgaga ctggaagaca tcaactccat
     5821 caggctggct tccatctcat gcactaaatc gtattcggca tcgcgggtca tcttcattga
     5881 ataggcattc agcgcgtcat aatcaaagaa gcctttgaaa atatcatcaa ggcagtaacg
     5941 cagaatgtta tccagaagaa tcattggctt gcgtcgacgc ggcgcttctg gcggtaaatt
     6001 cacaaagcgc ggcactttat ctgatgggat ctccagaagc gcgtaacgga tggtatcgcc
     6061 acggataatt tccaccgcca gataggtgta atcatctttc aggaactgca ctaagtcagt
     6121 gtcaggattg attaaaatcg gcgtaatgtg ctgacgcaga tactgcttaa aataatgacg
     6181 cagccagttt tgttgattga cggagagctg gcgttcatta atcaggaaga tctggttgcg
     6241 cgccatctcc agcaatagct cgttgtagag gccgtcgaat tcctgatcgg ctttcagcac
     6301 ccgggactga attttgccca gtaaatggcg ggaatgagag ttggagcctt gttcttcgct
     6361 aataatgatg cgtcgcttca gttcagcgaa gcggacttta tagaactcat caaggttatt
     6421 ggaatagatc cccaggaaac gcatcctttc aatcagcggg ttagatttgt ccgccgcttc
     6481 ctgaagcacg cgttcattga acgataacca actgagctct ttttcgatgt atagcttttc
     6541 ctgacccatt accactttta ctccgtttta ttcacgggac actgcctgga tattatggcg
     6601 agcaatattg gcagatgtcc aactatgcca gaaaaggggg aaaaaaactg ctggagcttg
     6661 cgatttacag gtgaattgaa aaacgccagc ggcagagctg gcgctttaac tacggggcat
     6721 tactcgtcgg cagcgtagcc ctgcggcggc agacgttgac catccagcca cgcggcgttt
     6781 tcgtgcattt tcagacgacc atcggcaaac cagctaatca ccagtggata aatggcgtgt
     6841 tcctgggttt gcacgcgggc ggtgacgtca tcctccgtat caccagcaaa taccgggact
     6901 ttcgcctgta aaataaccgg gccaccgtcc agttcatcgg tgacgaaatg caccgatgta
     6961 ccgtgctctt catcgccatt ttccagcgcc tgacgatggg gatgtaatcc gggatatttc
     7021 ggcagcagag aagggtgaat gttcagcaaa cgcccggcat agtgggagac aaatgccggg
     7081 ctgagaatgc gcataaaacc agccagcacg accacatcgg gtgcgtacat gtcgatttca
     7141 tgaatcaact cccggtcata ggcttcacga ctgtcaaacg cgctggcgat gagcgtatgc
     7201 gttgcaatac ccgcctggcg ggcgcgttca aggccgaacg cgtcggcctt attgctgaaa
     7261 actgcccgta cggtgccttt aattttgttg gttttacagg cgtcaataat tgcctgcaaa
     7321 ttacttccgt tgccggaaat aagcaccaca atattcatta ttcgataacc acgcgttgtt
     7381 cggaatcaga ggctttgatg ataccgattt tccacgcgtt ttcaccgttg gcattgagca
     7441 gggcgagggc tttgtccact tccggagcag gcagggcaat aatcatcccg acgccgcagt
     7501 tgaaggtgcg atacatttca tggcgctcaa cgttaccagc cgtttgcagc cagttgaaca
     7561 cttccggcca ctgccaggag gattcatcaa tcactgcctg ggtattatct ggcaatacgc
     7621 gcggaatgtt ttcccaaaag ccgccgccgg tcaggtgcgc aatggcatta acatcgacct
     7681 tttcaatcaa ctccagcact gacttcacgt aaatgcgggt cggtgccagc agatgatcgg
     7741 ctaatggctt accatcaagt tcggtggttt gcggatcaca accgctgact tcaagaattt
     7801 tgcgcaccag cgaatagccg ttcgagtgcg gaccgctgga accgagtgca atcagcacat
     7861 cgccgtcgct gactttagag ccgtcgatga tttctgattt ttctaccacg cccacgcaga
     7921 aacccgcgac atcgtaatcc tcaccgtgat acatccccgg catttctgcc gtttcgccac
     7981 ccaccagtga acagcccgat tgcagacaac cttccgcaat gccgctgatc accgctgaag
     8041 cggtatcaac atccagtttt ccggttgcgt aatagtcgag gaaaaacagc ggttctgcac
     8101 cttgcaccac caggtcatta acgcacatgg cgaccagatc aataccaatg gtgtcgtgac
     8161 gttttaagtc cattgccaga cgcagcttgg tacctacgcc gtcagtgccg gaaaccagca
     8221 cgggttcacg atatttttgc ggcaatgcac acagcgcacc gaagccgccc agaccgccca
     8281 tcacttccgg acgacgcgtt ttcttcacta cgcctttgat tcttccaacc agagcattac
     8341 ccgcgtcaat atcaacaccg gcatctttgt agctaagaga ggttttatcg gtcactgctt
     8401 gggtccccac gcgttacttg cggtagaaaa ataaaattcg gcgcaattct aacagggaaa
     8461 gcaaacgttt gcgagactgc tttacacaac ctttttgcac gtcttttccc caggcgcgcg
     8521 gcgaaagaag acttgtgcca gggcaaaggt tagttttcgg atggaataat cttctttcat
     8581 aaccatttga atataaaata actttatctc aaaccgttat cattttgact aaagtcaacg
     8641 aaaataatat tgccgccttg aagaaaggag gtataatccg tcgatttttt ttgtggctgc
     8701 ccctcaaagg agaaagagta tgaagatcgt ggaagtcaaa cacccactcg tcaaacacaa
     8761 gctgggactg atgcgtgagc aagatatcag caccaagcgc tttcgcgaac tcgcttccga
     8821 agtgggtagc ctgctgactt acgaagcgac cgccgacctc gaaacggaaa aagtaactat
     8881 cgaaggctgg aacggcccgg tagaaatcga ccagatcaaa ggtaagaaaa ttaccgttgt
     8941 gccaattctg cgtgcgggtc tggggatgat ggacggtgtg ctggaaaacg ttccgagcgc
     9001 gcgcatcagc gttgtcggta tgtaccgtaa tgaagaaacg ctggagccgg taccgtactt
     9061 ccagaaactg gtttctaaca tcgatgagcg tatggcgctg atcgttgacc caatgctggc
     9121 aaccggtggt tccgttattg cgaccatcga cctgctgaaa aaagcgggct gcagcagcat
     9181 caaagttctg gtgctggtag ctgcgccaga aggtatcgct gcgctggaaa aagcgcaccc
     9241 ggacgtcgaa ctgtataccg catcgattga tcagggactg aacgagcacg gatacattat
     9301 tccgggcctc ggcgatgccg gtgacaaaat ctttggtacg aaataaagaa taaaaataat
     9361 taaagccgac tttaagagtc ggcttttttt tgagtaaagc gcctataaca cataatacag
     9421 aggataatac tatgacgcgc cgtgctatcg gggtgagtga aagaccgcca cttttacaga
     9481 caatcccgct tagtttgcaa catttgttcg ccatgtttgg tgcaaccgtc ctggtgcccg
     9541 tcttatttca tattaacccg gcgactgtac tgttatttaa cggtattgga acgctgctgt
     9601 atctcttcat ctgtaaaggg aaaattccgg cttatcttgg ttccagcttt gcctttattt
     9661 caccggtatt gttactgttg ccgttagggt atgaagtcgc gctgggcggc tttattatgt
     9721 gcggcgtgct gttctgcctg gtttctttta tcgtgaagaa agcggggacc ggctggctgg
     9781 acgtgctgtt tccacctgcg gcaatgggcg caatcgttgc cgtcatcggt ctggagctgg
     9841 cgggcgtagc tgccggtatg gcgggtttac tcccggctga agggcaaacg ccagactcca
     9901 aaaccatcat catctctatt accaccctgg cggtcacggt tttaggttcc gtgctgttcc
     9961 gtggtttcct ggcaattatc ccgattttaa ttggcgtgct ggtggggtac gcgctctctt
    10021 tcgcaatggg aattgtcgat accacgccga ttattaatgc tcactggttt gcgctgccaa
    10081 ccctctatac gccgcgcttc gagtggtttg ccattctgac tattctgcca gcggcgttag
    10141 tggttattgc cgaacacgta gggcacctgg tagtaacggc taatatcgtc aaaaaagatc
    10201 tgctgcgcga tccaggtctg caccgttcga tgtttgctaa cggcttgtcg accgtgatct
    10261 ccggcttctt tggctccacg ccaaacacca cctacgggga aaacattggc gtgatggcga
    10321 tcacccgtgt ttacagtacc tgggttatcg gcggggcggc gattttcgct atcctgcttt
    10381 cctgcgtcgg taaactggct gccgctatcc agatgatccc attgccggtg atgggcggcg
    10441 tttcgctgct gctttatggt gtcatcggtg cttccggtat tcgtgttttg atcgaatcga
    10501 aagtggatta caacaaagca cagaacctga tcctgacttc cgtgattttg atcatcggcg
    10561 tcagtggcgc gaaggtaaac attggcgcgg cggagctgaa aggtatggcg ctggcgacca
    10621 tcgtcggtat cggcttaagt ttgattttca aactgattag tgtgctgcgc cctgaagaag
    10681 tggtgctgga cgcagaagac gcggatataa cagacaaata atgtaccgac cgggcagtgt
    10741 tgctgcccgg ttcaaacatc atgggattct gtggtaaact tctcgcgatt ttgtgaaatc
    10801 ctggttgagg tatctctgaa cacaccggca cagctctctt tgccacttta tcttcctgac
    10861 gacgaaacct ttgcaagttt ctggccgggg gataactcct ctttactggc cgcgctgcaa
    10921 aacgtgctgc gtcaggaaca tagcggttac atctatctct gggcacgcga aggcgcgggg
    10981 cgcagccatc tgctgcacgc ggcttgcgcg gaattgtcgc agcgtggcga tgcggtgggc
    11041 tatgtcccgc tggataaacg cacctggttt gttccggaag tgctcgacgg tatggagcat
    11101 ttgtcgctgg tctgtatcga caacattgag tgtattgcag gcgatgagtt gtgggagatg
    11161 gcgattttcg atctctacaa tcgaattctg gaatcgggca aaacacggtt gttgatcacc
    11221 ggcgatcgtc caccgcggca gttgaatctg ggattaccgg atctcgcgtc gcgactcgac
    11281 tgggggcaga tctacaaatt gcagccactt tctgatgaag ataagttgca ggcgctacag
    11341 ttacgcgcgc gtttgcgtgg ttttgaactg ccggaagatg tggggcgttt cttgctgaag
    11401 cggctcgaca gagaaatgcg cacgctattt atgacgttgg atcagttgga tcgtgcgtcg
    11461 attaccgcgc aacgtaagct gaccattccg tttgtgaaag aaattctgaa gttgtagata
    11521 aggtgtttat tgtcggatgc ggcgcgaacg ccttatccga actacgcgaa gttaatccat
    11581 agaattcgta ggccggataa gacgtggcaa gcgtcgcatc cggcaatgtg cttcaactag
    11641 cgatggagca catcttaacc cacgatttcc agtacctgct ccggtggacg accaatccgc
    11701 gctttgccat tcgcgaccac aatcgggcgc tccatcagct tcggattatc caccattgcc
    11761 tgaatcagcg cctcttcgct aagcgaactg tcagccaggt tcagttcttt ataaagatcc
    11821 tctttctggc gcatcaattc acgggcgcta ttcatcccca gcattttcag caaatcgcgc
    11881 agcgttgccg catcggcggg tgtctcaagg tagagcacca cttctggctc cacgccgttt
    11941 tctttcagca ggttcagcgt ttcccggctc tttgaacagc gtgggttatg gtagattttt
    12001 acctgtttgg tcattgttct tcctttaatg cgaattacat cttggtataa ggcttaaagc
    12061 gttcctgcag ctggcgcaac tggtcgatgc gcgcatcgta acgcgcttgt tgcaggctgc
    12121 ctaatttcac ctgcgaactg gcgctactca acagcgaaat ggcctgatcg agtcgtccgg
    12181 cgagcgcata accttctgct cgcgcagcca gctcctgatc gcggttattt agcgcggctt
    12241 ccgcctgtgc cagcaaatcc cagccgttgc tgtcatcttt attattaaag gtgtagcgat
    12301 taagaatatt ggccgcttct tgtggttgac cgccttgtag ataagcgttc gccaggttga
    12361 gctgcaacac aggattggtg cgcaaatcgc gggcattttt cagacgattg atcgcctcat
    12421 tggctttgtt ttgcccaaga tcgatatcag tagccagatc gagataccat gcgttgccag
    12481 gttctgccgc cagtaacggt tgcagcgttt ttcgcgcctc gtcgtattta ttggcttcca
    12541 tcgcctgtaa agcacgacca tattgcgccg ctcgttgctg acgaacgttt cctttcgccc
    12601 attcatccag caaatcactg gtgagctggt tacgtccgga attatacatc cccagtgtgc
    12661 gcgctttcgc cagatagaaa tcttccgacg actgcaccac catcgggcgc atctgattag
    12721 cacggttgcg ggcatctgcc agacgacttt ccggcaacgg gtgagtcagt aaaatttccg
    12781 gcgggcgcga ggagtaacgc gcctgatcga gtaatttttc gaggaaggtt ggcatcgcct
    12841 gcggatcgaa tcccgagcgt tgcagcacct gaataccaat gcggtccgct tcctgttcat
    12901 tttgctgggt gaaactgatc atcccctgac gcgttcccgc cagtgtaccg gtcagcgccg
    12961 ccatccccgc ctgcggactg gccatcgcca gtaaaataga acctaacgcg ccgacccagg
    13021 tcagcggcgc gttgcgctgc tgatcttcca tcgctcgcgc caggtgacgt tgggtgacgt
    13081 gggagatttc gtgcgccata actgaagcca gttgactttc gttatcggaa taacggaaca
    13141 gggcagagtg cagcaccacg ttgccgccaa agaaagcaaa ggcgttaatt tcgtcgttgt
    13201 tgatcagaaa gaaatgaaac ggtgtcttaa ccgaattggc atgcgaaacc agacgcatcc
    13261 ccagcgaatt aatatattgc gttaacagcg ggtcattaat taacggcgcg ctgccgcgta
    13321 gctggcggac ataatagtcg cccatctgca tttcctgacc aatggaaagc gtgcttcctg
    13381 cggaggttcc catatccggc aaggtgtctg cgctgtcggc aaatgccggg gctacctgac
    13441 caatggtcat agcagcaatg agggttgcaa ccaggttttt tttcaactgc ctgaacataa
    13501 cctctatcct gtatttctga agatagtcat ttgaccgata ctgtgaaata tcgttcccac
    13561 cgcgccgcct ttgagtgtaa cggcgaagat tagcccactc aaatgacttt tcactttcct
    13621 gaatccgtca taaaaagcga agtctttttt gtgacatttc gatacaattg cggatcgcaa
    13681 tcccgctctt gaggttcagg gaaggtcttt atgctcgaaa tgttgatgca atggtatcgc
    13741 cgccgtttta gcgacccgga agcgattgcc ttgctggtta ttttagttgc cggatttggc
    13801 attatctttt tctttagtgg cctgcttgct ccgttgctgg tggctattgt gctggcctat
    13861 ttgctggaat ggccaaccgt gcgcctgcaa tctattggct gctcccgccg ctgggcgacg
    13921 tcgattgtat tggtggtttt cgtcggtata ttgctactga tggcgttcgt ggtactgcct
    13981 atcgcctggc agcagggcat ctacttaatc cgcgatatgc cggggatgct caataagctt
    14041 tctgactttg ccgccacgtt gccgcgccgc tatccggcgt taatggatgc gggcattatt
    14101 gatgcaatgg ccgaaaatat gcgcagtcgg atgctgacca tgggcgattc ggtggtgaaa
    14161 atttccctcg cctcgctggt cggtttgctg accattgccg tctatctggt gctggtacca
    14221 ttgatggtct tcttcctgct gaaagacaaa gagcagatgc tgaatgccgt tcgccgggtg
    14281 ctgccgcgta accgtggact ggcaggacag gtgtggaagg agatgaatca acaaatcacc
    14341 aactatatcc gcggcaaagt gctggagatg atcgtagtag ggatcgccac ctggctggga
    14401 ttcttactct tcgggctgaa ctattcgctg ctgctggcgg tgctggtcgg cttctcggta
    14461 cttattccgt acattggcgc atttgtggtg accattccgg tggttggcgt ggcgctattc
    14521 cagtttggtg caggcactga attctggagc tgtttcgcgg tgtatctgat cattcaggcg
    14581 cttgacggca acctgttagt accggtgttg ttctccgaag cggttaacct gcatccgctg
    14641 gtgattattt tatcggtggt gatcttcggt ggtttgtggg gattctgggg cgtattcttc
    14701 gccattccat tggcgacgct gatcaaagcc gtgatccacg cctggcccga tgggcaaata
    14761 gcgcaagaat aatcttcagc ccgacgaact gtttcgccgg gctgattttt caggattcct
    14821 gacgtaaata aatagcccga taacaaatac tcaccagcac tgcaccgccg ataatattac
    14881 ccagcatcac cgggagcaga ttggcggtaa taaaatggct gaccgtcagt gcaggaaaat
    14941 tgtctgcgct actgtgcgcc agttgccaga aggaggtagg ggcgaaatgg cgaatggcaa
    15001 tggcgaacgg aatcacaaac aaattggcga tacagtgctc aaagccactg gcgacaaaca
    15061 gggtgatggg caaaatcatg gcgacgattt tgtcgcataa cgaacgcccg caataactca
    15121 tccacagcgc caggcaaacc atcagattgc acataatgcc gaggctgaca gattcagtaa
    15181 atgtatgatg cattttgccc tcggcgcagt gtaaaaccgc gactccccac atcgcgtttt
    15241 cactcatcac cagcccggaa aaccagatta acaaactgaa acaggcaata cctgccagat
    15301 taccgcaggc caccagaagt gcgttaatca gccaagttcg ccaactaata acgccccgac
    15361 tttttgccat caccgtcatt accgacgagg tgaacaggct ggtgccgcaa accgccagca
    15421 aaataaagcc gagtgtaaag caaaggccgc ccaccagatg ggttaatgcc tgtgaagacg
    15481 gggcatcggc gataacggaa aggtaaaaag taaatccgat cgccataaac acgccagcgg
    15541 acatcgccat taaaaagacc gacactttac tgcgggcaat tttatgggcg gcaatccgtt
    15601 cagcgattgc cgcttttctg gcgctcaact gcaagtcgaa agagagtttg tttctcatgc
    15661 atactcctgc aaaagcagat tacaacacct cgcgaaccga gatccccaga cgctgcattc
    15721 gtgacagcag cgtggtgcgc ttcatcccta atcgtgtcgc cgcgccacgg ggcccggcaa
    15781 caatgccatt ggtttctcgc aatacctgaa caatgcgctg acgttcttct tcgtcattct
    15841 ctggcgtcgt cgggtgcagc aactgcgcca ttgaactgcg aagcgctgaa tcttcattta
    15901 gcgtcggcag taaacggctt tgtcggacat taagatgtaa attcagactg ttaccgcgag
    15961 tcagcagtac cgctcgctca atcacgtttt ccagctcgcg cacgttgccc ggccaatccc
    16021 acgacatcaa ctggcgtagt gcctcggtcg ggatggcgtc aattgcgcga ttcatatggc
    16081 gcgccatttt ttgcgtgaaa tgttttgcta aaagagggat atcttccgga cggtctcgca
    16141 gcggcggcaa ttccagtggg aagacattca ggcgataaaa gagatcgctg cgaaactggc
    16201 gatcttcaac catttgccac aaatcacggt tggtggcggc aatgactctg acattcaccg
    16261 ggatcgttct actcccgccg agacgctcaa tttcccgctc ctgcaatacg cgcagcaatt
    16321 taggctgaag ttctaacggc agatcgccaa tttcatcgag aaacaacgta ccgccatcgg
    16381 caatttcaaa acgaccacga tgggtattaa tcgcaccagt aaaggcccct ttgtcatgac
    16441 cgaataactc actttccaga agactggcgg ggatggcagc gcagttgatt ttgaccagcg
    16501 gcttgtcgcg tcgcgggcta agttgatgga tcgctctggc gatcacctct ttgccggttc
    16561 cggtttcacc acaaatcaac accgtactgt cgctcttcgc cacaatatct acctgctgga
    16621 gcaggtcttc cattgcctgg ctttgataga taatgtcacc gacgcccaga ttcgaaagga
    16681 gctgctcgct aagctggtgg ttttcttgct gcaaactttc ctgcaaatcg gtcatgctac
    16741 gccaggcatc ggcattgcca acggcaatag cgatgcgatc ggctatgtgt tgtagtagct
    16801 ggcagttttc ctcactaaag agagtggtag aggtatgcgc cagcaacaat gcacccggtg
    16861 tatggttgcc aaaggtaagc ggtatcagga gcgaagattc gcagccgtta gatgcaagta
    16921 agtgtaataa cgggtcgcgt tgccagagaa acagatcgtc tgcacggtgc gtcaaggtcg
    16981 gttgtcgggt ttgtagcgtt tgtgtcagca atacactgtc gccaggcata ttgcgtggca
    17041 gacacgcaca atgtgaggca gaaagatcgc tgcaccacag gctgaacttc tcgttctttc
    17101 gatgatcgcc cagtaccata ctgactgaag ccagaccgaa aaaatgatgg atctcacgag
    17161 cgacgtcagc gatcagatcg tcgagatcaa gatgtgacag cactgcatta gtgatatcca
    17221 ccagtacccg atgatgatcg cgatctttct ccagcaaacg cagcgccacc tgttgttctt
    17281 catcattgag ccatgattgc agaaccgggg tgataatgcg tgccagccag ttaaacagtg
    17341 tgcaatcctg gggagtaaaa cgactgccgt gggtgcgaat aaagcgtagc tccgccattc
    17401 cactactgcc gagaaggacg gcgtgacgcg aataatcgac catctcgccc tgatactctg
    17461 ttgttgccgt cacatcgctg ccatcatcat ggtagcggta atagaagttg cttttgagaa
    17521 catgcagttc aataagttgt accgggctga acggcggcgg cagcgggatg aaggcgcgta
    17581 ataaagacgc cttgccgtgt ttctgcgcta accgctctgc gagcattccg tttaccgctt
    17641 caattgttat tccttgcggc ggggcaaaca tcgcctcgtc tgacatagcc atcaacagtc
    17701 cttattaatg agaaatcctt ctcacgcaat ttttctggcc atcagataga tagccggttc
    17761 cagcacaatt tcatccaaca gatgaatgaa ttgaatgcta cgcgcttgcc acacagacgt
    17821 cgcgtcatct gccagtggcg cgaatgcccg ggtcaacagg gcggtgtggc tggcgtcaat
    17881 aacaatctca ccaaaagtca tcacccccag catcttacgt cgggcttgct cctcttcgac
    17941 cagtgcaagc caatcttcgt attccgtcag tgggcagcgg aaggtgacat tcagacaatc
    18001 aatcacgcca acgtgatgac cgatagccag cgagtaatac attacctggg agctgtgttc
    18061 cggcatctcg tcgctactgg cgacaaactt ttttcgcagc gtccagaaaa caatttcccc
    18121 gcactcttca gtcatgtaac ccccggagta cgcgaaagag ttgctgaacg atactattga
    18181 gacgtggatc gtcggcatcg cgcaaccagg tgttaacccg atttcctgta ggatcgttgc
    18241 ttaaatggcg caggagccga tcgcaaattt cttgcccctg acgatagccc gccagccgcc
    18301 gcgcttctcg ctcaatggcg atacgctgtg atggcgggat ctgcggccac aacggttgca
    18361 tagtcacccc ggtgggatcg cgataatcca ccgcgtgaat cttctgttgc agcaaaccga
    18421 gcgccacggc gaaaccgtga atggtggcgg ccggtgttgg cgggcagccg gggatccaaa
    18481 catcaatggg gacaatggtg tcgctgccgc cccagacgct gtagagatcg tggaaaatac
    18541 cgccgccgac accgcacgcg ccgtaggaaa cacagatttt atgatcgggg gcagactcat
    18601 acgcccgaag tgcaggcata cgcatcgccc gggtgactgc gccagtaaat aacaaaatat
    18661 cggcgtgacg cggtgatgaa acaaccttaa tgccaaaacg ttctgcgtcg aatacgggtg
    18721 tgatggcggc aaaaatttcg atttcacagg cgttgcagcc gccgcaatcg acgcgataaa
    18781 cgtaggctga gcgacggata tcctgtagca aatgccgctt catcttttgc gtttgctcgt
    18841 ccagcgtgat gggctggctg acatgttgtg taagcactgg actcatagct gctccttagc
    18901 caccagcggt acatctgtat cgtcgttgat cagcgtcgcg cgttgtttgc actccgggca
    18961 gacgctcgct tgcgcccgca acatttcgcg gttttgtggc gcattttgtt gctgtgccaa
    19021 caattcagca gccagtgcga cggttttttg cggggcaaac gggcgttcgc aacggctgca
    19081 acgttgtaga tggaacgtcg cgcgggtata gagatcggct ttattggtga cggtcagttc
    19141 aaagttattg gtaagctgga tggctctggt cgggcacact tcttcacaac gtccgcagta
    19201 aatacaacgc cccagataga gctgccaggt gcgcgaattt tgctggtcgt cggtctggat
    19261 agtcagcgca tttgtcggac aagcacaggc gcaggcacca caggcaatac attggctggg
    19321 catcaggtcc ggtttcccgc gaaagcctgg gctgacctcc agtggcgcga agggatattt
    19381 caccgtcgcg gttccggcgc gcataatagt tttcagtaac ttcagcatct tctgtcctta
    19441 cttcagcggc gaacggttac gatcaatgcc gtagcgttcg atctctttat acggcacggt
    19501 ttttgactgg cgcttgcgca catcaaccag cgtcacacgg tcggtacagg agtagcaggg
    19561 atcaaggcta ccgataatca gcggtgcgtc agaaacggta ttgccgcgca gcatgtaacg
    19621 caacaccggc cagttggcgt aggtggcggc acggcagcgc cagcggaaca atttttggtt
    19681 atcaccgagc atgctccagt gcacgtcttc accgcgtggc gcttcaacaa agccgagcgc
    19741 gaatgcgtga ggtttatagc taaagccttc ggtcagcagt ggggtatccg gcatgttgtc
    19801 gagagcaaat tccagcattg ccagcgaatc aaacgtctct ttgacacgga ccatcacgcg
    19861 ggagaaaaca tcgccgccgg tgaaggtaaa cagtgttttt ggaatattgc cgtagtcggc
    19921 gtaggggtga tcaaagcgca aatcacgggc aaaaccactg ccgcggatca gcggccctac
    19981 agggctataa tcacgggcga tttgtcggtc gagaatgcca atgccctgag tgcgttgttc
    20041 catattcggc gtggcaagca gcatttctac cagctccgac acgtcggcgc gcatctcgcg
    20101 caccagtttc agcgtttgca gacgttgctc tttgagaata tcgcggcgaa caccaccaat
    20161 cagattcaga ccgtaggttt tacgcgaccc ggtcagcaat tccgccattg tcatcgactt
    20221 ttcccgcacg cggaaaaatt gcataaagcc ggtatcaaat ccaacaaaat ggcaggagag
    20281 gccaaggttg agcaaatgac tgtgtagccg ttcgacttcc agcagaatcg agcgaatggt
    20341 atgcgctcgt tgcggcacct caatccccag tgcattttca accgagttgg tataggccac
    20401 actgtgggca aaaccgcaaa tcccacacac gcggtccgat aagaaggtca cttcgttgta
    20461 gcccatccgc gtttctgcca gtttctccat gccgcgatgg acataaaaca gacggtaatc
    20521 agcatcgaca atttgctcgc catccacaaa caagcggaag tgacccggtt catcggaggt
    20581 gatatgcagc gggccgacag ggatcacccg cgcaccgctg ttgccctcat tgataaacgg
    20641 atacgtttcg gaatcagtcg tcggttcagg gcgcagtcga taatccatcg catctttgcg
    20701 cagcggatgc atatcttccg gccagtcatc gggcaacacc aggcgacgct gatccggcag
    20761 gccaaccgga atcagcccgt acatatcacg aatttctcgc tcgccccaga ccgcggcagg
    20821 gacgcgcggt gtgactgacg gaaactcccg actgtcggca tcgaccagcg ccttcaccac
    20881 aatccagcat ttttcggccc cttccattga aagggcataa taaaccgcgt aatgaccgtt
    20941 aagtgtccgc tcgtcgttgc caaacaggac cggaagccag ccatcatgtt gataataaag
    21001 atactgtaca acgtcaggca gcagattgat tttcaccgta atggtgacct gttcaggcgt
    21061 ttgtcgctct tcatccagca ccgcgccggg gaactgcgtt ttcagggcgg cgagaatcgc
    21121 ttcgccacga tttgacgatg aattaacgtt cactgtgttt ctccgaaaca gatgcggtgc
    21181 ctgaaggcaa aaaatcatgc caggtgctac gttgtgcagg caggtcgtgc gtccctgaga
    21241 ggactatagt ggaagcgccc gccaggatcc tgatgacagg ttgtggaata tgcgttccca
    21301 tcgccagcat catgaccagc agaatcacca ttggcgaggt ggtcaaccag ccgagatcac
    21361 cccggttaac ggcctgcggc ggtttcgcca ttaacacccg cgcagccatc cgtaccaggc
    21421 ccgccagcac cagcgttaac agcaataaca gcaggacgat aatcagcagg tgattacgtg
    21481 ccagtccggc ggtaacggtc ataaattcgc taagaaaaat gttgaagggc ggcatccctg
    21541 ccagcgccag cgcaccgccg ccaaacagca cggcggtaaa tggcatgatt ttgagcatcc
    21601 cacagacgac gttgagatcg cgcgtgccgt acttgagcag tacattgccg gaaccgcaga
    21661 acagcagcgt ttttgccaga ctgtggttta aggtgtgcag cagcgcggca aaaattccca
    21721 gcggcccgcc aatgcctagc gccaccgcga ccagtcccat gttctccacg ctggagtacg
    21781 ccagcagacg cttaatgtcc cgctgtacca gaatgaaaaa tgccgccacg gcaaccgaca
    21841 acatgccgaa gatgagcaac aaccggttgg ggaaatcgct gccgatggcc tggcaaataa
    21901 tgatgtaata gcgaatcagc accaacagcg cgcagttcag caatacggcg gagagtaggg
    21961 cgctgaccgg actcggcgct tcactgtgag catccggcag ccaggcgtgc atgggaaata
    22021 gcccggtttt ggtaccaaag ccaattagca caaacacaaa ggccaacagc attaatgttg
    22081 gatcaagcaa ggacgattgc ttaagaacct cgctccagaa tatcgccatt tctgcctgcg
    22141 gcataacgct ggcggcgttg gcgtatacca gcacggtacc gaacagacca aaagcgacac
    22201 caacagtaca aataatgatg tacttccatg ctgcttccag cgatgaacgc tgaccgtaaa
    22261 tgcctaccag aaacgccgag cttaaggtgg tggcttcgat cgccgcccac atcacaatca
    22321 ggttattgct ggtaacaacc agcagcatgg tgaacaaaaa cagatggaag aaaccgtagt
    22381 aatcgcacag cgtaacgggt gaaagctcgc cgtgctcaac ttcatgacgc atgtagccaa
    22441 tcgagtaaac accggtgaga aagccaatca caccaagaat ggcgaggaac aaaccgccca
    22501 gaccatcaat atgaagccac agtcccgcag cgaatatttc tcctgcatca gcggcagttt
    22561 ggaccaccca gagtgccaga atcagcagca gtgtgatccc taagctatgt aatactgtca
    22621 ccgtgcgagt cgcagagtgc cctcgtttcc ggcaggcaaa acagagcagc gaaaaaagca
    22681 gcggagtgag caggagtaaa gcgaacatca cagaataact catctcatta tcccttcagc
    22741 gcggtcaagt tgttcacgtc cagcgtgccg tgggtacgcc atatttttct tgccagtaac
    22801 accatcacaa tgacggcgaa gatggcgtcg gtagcgatac caatttccac cagttccggt
    22861 gcacgccagg ctagaagcgc cagcaccaga tgggagccgt tttccatcag gcagtaacca
    22921 aaaatttgcc gcaggatatt gcgctggcta acaatgcaca gcaggccaag cagaaaatga
    22981 cctaacgcca ccgccagcgc tggtttcagc ccggtagcca tcggtagctt cacgggctga
    23041 acgacaaatg cgcaaagcag gacaattaac gccgcgagca gtgccatcat tgccggaccg
    23101 aataacgctt tttccgggat gttctgggga atatttcgtg cagcgtaagt catgattaac
    23161 ggtaccagca gcactttggt gataaaggcg ctggcggacc agatcagcag ttgctctgcg
    23221 gcgaacaggc acgagagggt ggcgaaaata gacaccagca ccagcgactg gcaggcgtaa
    23281 aatccgcatg acaggcgata gcttttgacg ctaatcacaa acagcgatgt cagcatcatc
    23341 agtcccgcca gattatttac gatcatagaa ccggtcattc atcatctcct tactgcaacc
    23401 aggtcgcggc gattacgctg gaaatcagcg acatcacaat cagtacaatc agcaccaggc
    23461 gcattgaccc tggcagcggt gcggcatcgg cgacggcctc gctcggtttg ccggggacga
    23521 cgcgaccaaa ccagcgaata aaccaggcga aactggcgac cgattcaatc atcagcagaa
    23581 tcatggcggg cagcaggatc cagtactcca ctgacaacgc aaaaccggca gcaaacagcg
    23641 ggaatttact gaagaagccg ttgaacggcg gtacgccggt aatcgccagc gctgccacgc
    23701 agaaacccac gcctggcaac ggcagggtgt gcaatacgcc acgcagacgc ggcaacaagc
    23761 gcgtgccgca gctgtaactc agcgcacctg ctacaaggaa aaacaggctt ttagcgaacg
    23821 cgtggttgac gatgtaggcg atgctgccct ccagcgccag ccgcgagccg aagatggaga
    23881 gcgacaagcc gaagaacatc cagccaagtt gagtgatggt cgaccaggcc agcaaccgct
    23941 tcatatcctg ctgtggcaaa tacatcagaa atccataaag aatggtgacc agggccatga
    24001 ccatgccaac gccgccaatc acatgcggga tattgccgcc gtcgataatg gcgcgggcaa
    24061 aaatgtaaac gcccactttc accatcgatg cggcgtggag ataggcgctg atcggtgttg
    24121 gcgcttccat tgcgtccggt agccacgctt gcatcggtag ctgggccgat ttcccccacg
    24181 cggcaaacag gatgccgcca taaaccagat aacgtgcgtc gccgtgtaac tcgctcatcg
    24241 cgctaagcgc aaacgttccg gtctgcaaaa acagcgtggc ggcggcaaga tacaacccca
    24301 acgaaccgat atgggtgata agcaacgctt ttagtgctga acgctgcgct ttatcgctct
    24361 gataataact gatcaacacc caggagcagc cgcccgtaat ttcaaaaaac aacaactgac
    24421 cgagcagcgt cgaggagagt accagtcccg ccatcgcgcc gataaacacc agcaaaaatg
    24481 cgtaataacg attcgtgccg ttatgcgggt gttcgcgatt tttatccgtc agataacccg
    24541 tggagtagat cgtgaccagc aaaccgagaa acaccaccac aaacagaatc agcgtacaca
    24601 cgcgatcaat gaccaggcca aacaatgcca catcccccac gttgaccaac gtcaacgtga
    24661 cggcaacttt atcggcctga taaaacgcgg agatcagcga caacatgcac agcgtggtca
    24721 gcgcggcgaa caaaaccccc cattcggcgg cccgacgttg tggcgaaaac gaaacgacca
    24781 gtgcgccgat aaaaggcagc aataacgtcg tcagagcaag attttccata ttccgtcagt
    24841 gctccttaca gaccggttaa ccagaagacc catgcaagcg cagcaaggct gaagccaagc
    24901 caggtcacat ggtgaatgag taaaaaacgc ccgcgtgcca gcgtgttttc tgcgattgag
    24961 gccagtacaa aaatcagcaa aaccttgagc agcgtaacga caagtgaagt cagcaggcag
    25021 gcgagagaaa gttcttgcgc gcacccaaag ggcagaaaca gggccacaaa cagtgctgcc
    25081 atcacgacct gtttcagccc cagcccccat ttcgccagcg ccagcccggc accggaatat
    25141 tctgtcagcg ggccttcctg taattcctgt tctgcttcag caacatcaaa gggaattttt
    25201 cccatctcaa tgaagcaggc aaaaccacag gccagtaacg ccagtacggt ggttagcggc
    25261 gagttccagc ccgttgccag cgtattgctg atcatctcga tatgcgtgga acctgctatc
    25321 agcgccaata ccagcagtga gagaatgagc attggttcga ccagaatgcc gagcgtcaac
    25381 tcgcgactgg caccgactcc cgcaaacggg cttccggtat ccagcccgga aagagcaaag
    25441 aaaaaacgaa acagggcaag aagatagata agggtgatca gatcgccgcc gcccgcaaaa
    25501 ggggaaacgg taataaatag tggtagggcc atcgccagca ccagcatgct gctgattaat
    25561 acccacggca tcaggcggaa catcagacct gaagatatag gcgcaacttc ctggcgttta
    25621 aacagtttgt ggatatcgcg ataatcctgc cagatccccg gcccgcggcg ggagtgcata
    25681 cgcgcgcgta tctgccggga aatacccgta aaaagcgggg ttagcatcag cagaatcacg
    25741 gcctgtgcta acgcaaaaat gaccagatat ccgtcgcaaa gagtttgtct catggtgatt
    25801 tccttagacg gcaatagcga ttagcagcac aaccagtgcg gcgaccacat acaggcaata
    25861 gagacgaaag tcgccgcttt gcagatgctg tatttctttg gccagccgtt gggtggcgct
    25921 cacgatgggg cggatcaccc gctcatccca gaagggttct gtgctctgag ccctggcggt
    25981 gacgtgcgca agacctttat ttagcctcag cgtagggtcg agctgttgtc gtagacgaaa
    26041 tagcgcagag aagaccacac gcagcggctg catcacgcca ttgcctgacg gggccatcgc
    26101 attttcccag ccgtagccgc atgcccaggc atctcctgtg cgacgaaacg ctgcgcggcg
    26161 cgaacgacac cacagccagt aaaggccagg cagcaaaggc atcgccagta gtaacagcaa
    26221 cagtaatgag ggggtgacct gtgtatgaaa cgtgccgggt acaagtgcta ttccgctggc
    26281 gacagtgacg ggaggggtat tggtaaacgc atgggcaata tgcattatct tcggtgccag
    26341 ccaactggca ctaagcgcaa ttaatacgca gagtgccgcg agcagtagca tggcgacgat
    26401 catcgtattt ggcacttcct gtgcctcttc agcgtgtgta ctgcgcggcg caccacagaa
    26461 agtaataccg tacattttga cgaagcacat tactgccagc ccaccagtga ctgccagcat
    26521 caccatagca ataggacccg caagttgtag cgctacggct tccacacgac ttagtgagaa
    26581 cagcgactgc caggtgtacc attcgctgat aaaaccattc agcggaggaa gggctgatat
    26641 cgcgaggcaa ccaatcaggc atgctgcggc tgtccacggc atccgtttcg ccagtgcccc
    26701 cattttttcc atgtcgtggg tatgcaaacg cgaaataatc gcgcccgcgc cgagaaatag
    26761 cagccctttg aacagcgcat ggttgagcag atgaaacagt gcgccgagca gtccaaccac
    26821 ggtgagcagc gggtcgtgta gtgacagacc gaccatcgcc acaccgactg ccagcaaaat
    26881 aatgccgacg ttttcgacgg tactccaggc cagcagccgt ttgatatcct gttccgccag
    26941 cgcatacagc acgcccagga gcgcggagat tgcgccgatc gccatcacca gaatgcccca
    27001 ccacagcggc aaacccgttt gcgccagcag atccatcgcc actttcagga tgccgaaaat
    27061 accgattttg accattacgc cagacatcaa cgccgaagcg tgcgatggtg cggcagggtg
    27121 agcgcgcggc aaccagctgt gcaacggcat catcccggct ttcgcgccaa aaccgaaaaa
    27181 ggccagcagg aacaccgccg acgccagccc cggagaaagt gaaagcgtgc ggaaactggc
    27241 aaaatctagg ctgccgcttt cgcgccccat cagcaagaag gcgatcataa tcagcaccga
    27301 tccggcgtgg gcgataaaaa agtagagcat gccagcgttg atcgacgttt tatcctgcct
    27361 ggcaatgacc agaaaccagg aagacagcga catcatttca aacagcacga tgaaccaaaa
    27421 agcgttgtcc atcaccagca gggcaaccat cgatgcgatg aaaatattca taaagaagcc
    27481 catcgccgcc gcgcctttgc cctcgtattc gcgcatataa gtcaatgaat agagcgaaca
    27541 aaccaccacc agcaaggaga taaccagcac cataaatgcc gagaggctat ccattcgcag
    27601 ggttaaatcg gcaaagtcaa aaggggtggc aaaacgggca actaaagtta cgccttcatg
    27661 taattgcgta attccgctga tgatgccaat cacgccgccc accagcgatg tgatgccgga
    27721 aagcttaata gccagtctgt ccagaccgag taaaaacagc gaagccagac tggcaaacag
    27781 atagaggatc agcgaccagg ttaataattg cagggcatcc attagcgttg ctcctgagtg
    27841 agggaaaggg gatcctccct gccattggca aaccagcttg cggcaaggcg ctgtttttct
    27901 ttcatctgac gttgcaggct atcgtcggtg atcagccgta acgcctgatt cgggcaagcg
    27961 cgaacgcagg caggaccttc tggcaagaaa tcacacaggt cgcatttcac cgcgacggtc
    28021 tggacacccg gttcccagcg cagcaaagca tgttgtgttg gcgcgttttc ttcgccgtct
    28081 ttgagtgagc cttcagcctg aaaaacatat tgcgcagggg cattcaccgg acggcttcct
    28141 gaagcgctga ttgcgccaaa tgggcagacc acggcgcaaa gcttgcagcc aatacagagg
    28201 ctttcgttga gttggatcgc atcatccctc tgggagatgg cattgaccgg gcagacctgc
    28261 acgcaagggg cttcctcaca gtgatgacac acgacagggg cagtgagtgt tgacgtcttc
    28321 gccagggcca ggcgcgggtg ttgctgtaaa ccttgcgttt tatgcacgtc cgaacaggca
    28381 gcgagacagg tattacatcc tgtacaccac agtggttcgg ccaccacaaa gcggttcatg
    28441 tcggctcctt gaaaatgata tgaaacattg catttcataa tgcataaagg atgccagtat
    28501 gattaattta atatattcaa taagttatga attaactgaa ttctgaacgg tacaaaaatg
    28561 atggtcatta acgttcagca aaaaatggat tcgacacccg tgtcgcaggg gaaagtgaga
    28621 atgtgatctt gatcacgata agtgaggtgt gctgaccgca agcgcagcca gcacggaatg
    28681 gagcaaagta atcaggcgtg ttctttcagc cagttcagca caacgtcgtg gtgattgctg
    28741 gttttgaaat cgtcaaagac atgttcgatt ttgccatcag cgtcaatcag gaagctgatg
    28801 cgatgaatac catcgtaggt tttgcccatg aaggactttt ccccccagac gccgaattgc
    28861 tcgcacacct ggtggtcctc atcagacagg agcgtaaagt taagcagctc tttttccgca
    28921 aaacgggaga gtttttcggg tttatcggtg ctgataccca gcacatcaac gcccgctttt
    28981 ttcaactcat ccatgttatc gcgtaagccg caggcctgta cggtacagcc gggggtcatg
    29041 gctttcgggt agaaataaac cagaacacgc tgtccctgga agtcggtcaa attaacttgt
    29101 tctccgtctt gatccggcaa gctaaatttc ggtgcgatat caccggcttt cagtggattc
    29161 attacttaac tccatcctgt tcatcatgtt gggaataatt gacgacgtta atactgcctt
    29221 gtgcattgag ttctgtacat agggctttga acgcttgctc aatatttgct gcgtccgcag
    29281 atgcggggct gtgggcagta atctgaatat gcaactgcgc agcccgttca ttttcagcag
    29341 gttgtgtgcg cgacaccagc tccgcaatgt tcatatgatg cgcgtcgaaa agtgctgtga
    29401 agcgttcaat taaatgcggg gagtctgcca catcgacctg aacccagaca gatgctggca
    29461 ttggcggacg cggacgcgcc gtcgtgcgct tcatcacgat taaaagatcc agttcggcac
    29521 ctttcaacgg taacgttgat tcaatcagag taatggcatt ccatgaaccg gaaagcagca
    29581 tgataaacgt gaactcttct cccagcatcg ccaggcgact gtcttcaata ttacagccgc
    29641 aactactgac atgacgggtg atggtgttca caattccagg gcgatcggca cccaacgcag
    29701 tgatcaccag ataatgttgc gatgacagtg tcaaactggt tattccttta aggggtgagt
    29761 tgttcttaag gaaagcataa aaaaaacatg catacaacaa tcagaacggt tctgtctgct
    29821 tgcttttaat gccataccaa acgtaccatt gagacacttg tttgcacaga ggatggccca
    29881 tgttcacggg aagtattgtc gcgattgtta ctccgatgga tgaaaaaggt aatgtctgtc
    29941 gggctagctt gaaaaaactg attgattatc atgtcgccag cggtacttcg gcgatcgttt
    30001 ctgttggcac cactggcgag tccgctacct taaatcatga cgaacatgct gatgtggtga
    30061 tgatgacgct ggagctggct gacgggcgca ttccggtgat tgccgggacc ggtgctaacg
    30121 ctactgcgga agccattagc ctgacgcagc gcttcaatga cagtggtatc gtcggctgcc
    30181 tgacggtaac cccttactac aatcgtccgt cgcaagaagg tttgtatcag catttcaaag
    30241 ccatcgctga gcatactgac ctgccgcaaa ttctgtataa tgtgccgtcc cgtactggct
    30301 gcgatctgct cccggaaacg gtgggccgtc tggcgaaagt aaaaaatatt atcggaatca
    30361 aagaggcaac agggaactta acgcgtgtaa accagatcaa agagctggtt tcagatgatt
    30421 ttgttctgct gagcggcgat gatgcgagcg cgctggactt catgcaatta ggcggtcatg
    30481 gggttatttc cgttacggct aacgtcgcag cgcgtgatat ggcccagatg tgcaaactgg
    30541 cagcagaagg gcattttgcc gaggcacgcg ttattaatca gcgtctgatg ccattacaca
    30601 acaaactatt tgtcgaaccc aatccaatcc cggtgaaatg ggcatgtaag gaactgggtc
    30661 ttgtggcgac cgatacgctg cgcctgccaa tgacaccaat caccgacagt ggtcgtgaga
    30721 cggtcagagc ggcgcttaag catgccggtt tgctgtaaag tttagggaga tttgatggct
    30781 tactctgttc ataagtcgcg cctggcaacg gttgcgggtg tttcgcttgt tttattactc
    30841 gctgcctgta gttctgactc acgctataag cgtcaggtca gtggtgatga agcctacctg
    30901 gaagcggcac cgcttgcgga gcttcatgcc ccggctggaa tgattttgcc ggtgacctcc
    30961 ggtgattatg caatcccggt gaccaacggt agtggtgctg tcggtaaggc gctggacatt
    31021 cgtccaccag cccagccgct ggcactggtt tctggcgcgc gtacccagtt cacgggcgat
    31081 accgcttcat tgctggtgga aaatggtcgt ggcaatactc tgtggccgca ggtggttagc
    31141 gtgctgcagg cgaaaaacta caccatcacc caacgtgatg atgctggtca gacactgacc
    31201 accgattggg tacaatggaa ccgtctggac gaagacgagc agtatcgtgg tcgttatcaa
    31261 atctctgtta agccgcaggg ttatcagcag gcggttacgg ttaaactgct gaacctggaa
    31321 caggcgggca aaccggttgc agacgcggct tccatgcagc gttacagcac ggagatgatg
    31381 aacgttattt ccgctggtct ggataaatct gccactgacg ccgcgaacgc tgcgcaaaat
    31441 cgtgcctcca ccactatgga cgtacaaagt gcagctgatg acaccggttt accaatgctg
    31501 gtcgtacgcg ggccgttcaa tgtggtttgg caacgtctgc cagcggcgct ggaaaaagtg
    31561 ggcatgaaag tgaccgacag cacccgttcg cagggcaaca tggccgtaac ttataagccg
    31621 ctgtctgaca gcgactggca ggaactgggc gcgagcgatc caggcctggc atctggtgac
    31681 tataaactgc aggttggcga tttagataac cgcagcagcc tacagttcat cgatccgaaa
    31741 ggtcatactc tgactcagag tcagaacgac gcgctggtag ctgtcttcca ggctgcgttt
    31801 agcaagtaaa aatacagggc tggaatcatc cggccctttt ttctgatatg atacgcaaac
    31861 gtgtgcgtcg gcaggaaaac gcgattttag cggtaattcg cacgaaattt gtttgtcgga
    31921 cgtagttcgg ataaggcgtt cacgccgcat ccgacaaaac atccggcaca ccagacagca
    31981 aaagatttta aaacgttaat tcacacccag gagtgataaa gatgcaaaag caagctgagt
    32041 tgtatcgtgg taaagcgaaa accgtataca gcacggaaaa cccggacctg ttggtgctcg
    32101 aattccgcaa tgatacgtca gcaggggatg gcgcgcgcat tgagcagttt gatcgcaaag
    32161 gtatggtgaa caacaagttc aactacttca ttatgagcaa actggctgaa gcgggtatcc
    32221 cgactcaaat ggagcgtctg ctctccgata ccgaatgtct ggtgaaaaag ctggatatgg
    32281 tgccggttga gtgtgtcgtg cgtaaccgtg ctgctggctc tctggtgaaa cgtcttggaa
    32341 tcgaagaagg tattgagctg aacccgccgc tgttcgatct gttcctgaaa aacgacgcca
    32401 tgcacgatcc gatggtcaac gaatcttact gcgaaacctt tggctgggta agcaaagaga
    32461 acctggcacg catgaaagag ctgacctaca aagcgaacga cgtgctgaaa aaactgttcg
    32521 atgatgctgg tctgattctg gtcgacttca agctggagtt tggtctgtac aaaggtgaag
    32581 tggtactggg cgatgagttc tccccggacg gtagccgcct gtgggacaaa gaaacgctgg
    32641 agaaaatgga caaagaccgt ttccgccaga gcctcggtgg cctgatcgag gcctatgaag
    32701 ccgtcgcccg ccgcctgggt gtacagctgg actgattttt ctgttcatca tcttgccgtg
    32761 ctgctggcac ggcaagacaa ccattctcgt aagatgtgca ttgaacgctc attcctccgc
    32821 catttcatcc cgttttcctg tggtaatctt cctctgaacc acctacgatc atttctataa
    32881 tgaatatatt gttgaggtat ctatgcgttg gcaagggcga cgtgaaagtg acaatgttga
    32941 agacaggcgc aatagctccg gcggtccgtc aatgggcggt cccggtttcc gtctgccgag
    33001 cggtaaaggc gggctgattt tactgatagt cgtgctggtt gcaggctact atggcgttga
    33061 tttaaccggg ttgatgaccg ggcagccggt ttctcaacag caatcaacgc ggtcaattag
    33121 cccaaatgaa gacgaagccg caaaattcac ctcggtgatt ctggcaacca cggaagacac
    33181 ctggggacaa cagttcgaga agatgggtaa gacctatcag caaccgaagc tggtcatgta
    33241 ccgtggaatg acgcgtaccg gatgcggggc gggccagtcc ataatggggc cgttctattg
    33301 cccggcggat ggtacggttt atatcgatct ctccttctat gatgacatga aagacaaact
    33361 tggcgcggat ggcgattttg cccaggggta cgttatcgcc catgaagtcg gtcatcatgt
    33421 gcagaaactg ttaggcatcg agccgaaagt tcgtcaactg caacaaaacg cgacgcaggc
    33481 ggaagtgaac cgcttatctg tgcgtatgga actccaggcc gactgttttg ccggtgtctg
    33541 ggggcatagc atgcagcagc aaggcgttct ggaaaccggc gatctggaag aggcgctgaa
    33601 cgcggcgcag gccatcggcg atgaccgttt acaacagcaa agtcaggggc gagtagtacc
    33661 agacagtttc actcatggca cttctcagca acgctacagc tggtttaaac gtggtttcga
    33721 cagcggcgat ccggcacaat gcaatacttt tggtaaaagc atttaacttc cggggcaggg
    33781 atggctgaac tgactgcgct tcacacatta acagcgcaaa tgaaacgtga agggatccgc
    33841 cgcttgctgg tgttgagcgg ggaagagggt tggtgttttg atcatgcgct taagttacgt
    33901 gatgccttac ctggcgactg gctgtggatt tcgccgcagc cagatgctga aaaccactgt
    33961 tctccctcgg cactacaaac tttacttggg cgcgagttcc ggcatgcggt attcgacgcc
    34021 cgccacggct ttgatgccgc tgcctttgcc gcacttagcg gaacgttgaa agcgggaagc
    34081 tggctggttt tgttactccc tgtatgggaa gagtgggaaa accaacctga tgccgactcg
    34141 ctgcgctgga gtgattgccc tgaccctatt gcgacgccgc attttgtcca gcatttcaaa
    34201 cgcgtactta cggcggataa cgacgctatc ttctggcggc aaaaccagcc gttctcgttg
    34261 gcgcatttta ctccccgtac tgactggcac cccgcgaccg gcgcaccaca accagaacaa
    34321 cagcaaatct tacagcagct actgaccatg ccgtcgggcg tggcagcggt aactgctgcg
    34381 cgtgggcgcg gtaaatcggc gctggcaggg caactcattt ctcgtattgc gggtagtgcg
    34441 attatcactg cgcccgcaaa agcggcaacg gatgtactgg cacaatttgc gggcgagaag
    34501 tttcgcttta ttgcgcctga tgccttgtta gccagcgatg agcaagccga ctggctggtg
    34561 gtcgatgaag ccgcagccat acctgcgccg ttgttgcatc aactggtatc gcgttttcct
    34621 cgaacgttgt taaccactac ggtgcagggc tacgaaggta ccggacgtgg ttttttgctg
    34681 aaattttgcg ctcgctttcc gcatttacac cgttttgaac tgcaacagcc gatccgctgg
    34741 gcacagggat gcccgctgga aaaaatggtt agtgaggcac tggtttttaa cgatgaaaac
    34801 ttcacccata caccacaagg caatatcgtc atttccgcat ttgaacaaac gttatggcga
    34861 agcgagccag aaacgccgtt aaaggtttat cagttattgt ctggtgcgca ctaccggact
    34921 tcgccgctgg atttacgccg catgatggat gcaccagggc aacatttttt acaggcggct
    34981 ggcgaaaacg agattgccgg agcgctgtgg ctggtggatg aggggggatt atctcaagaa
    35041 ctcagtcagg cggtatgggc aggttttcgt cgcccgcggg gtaatctggt ggcccagtcg
    35101 ctggcggcgc acggcagcaa tccactggcg gcgacattgc gtggacggcg ggtcagccgg
    35161 atagcagttc atccggcgcg tcagcgcgaa ggcgttgggc aacagctcat tgccagcgct
    35221 ttgcaatata ggcctggcct cgactatctt tcggtgagtt ttggttacac cggggagtta
    35281 tggcgtttct ggcaacgctg cggttttgtg ctggtgcgaa tgggtaatca tcgtgaagcc
    35341 agcagcggtt gctatacggc gatggcgctg ttaccgatga gtgatgcggg taaacagctg
    35401 gctgaacgtg agcattaccg tttacgtcgc gatgcgcaag ctctcgcgca gtggaatggc
    35461 gaaacactcc ctgttgatcc actaaacaat gccgtccttt ctgacgacga ctggcttgaa
    35521 ctggccggtt ttgctttcgc tcatcgtccg ctattaacat cgttaggttg cttattgcgt
    35581 ctgctacaaa ccagtgaact ggcattaccg gcgctgcgtg ggcgtttaca gaaaaacgtc
    35641 agcgacgcgc agttatgtac cacacttaaa ctttcaggcc gcaagatgtt actggtccgt
    35701 cagcgggaag aggccgcaca ggcgctgttc gcacttaatg atgttcgcac tgagcgtctg
    35761 cgcgatcgca taacgcaatg gcaatttttt cactgactcc ttcaattttc tcccatggtc
    35821 attgtgagtc agcggcgtaa aattaatgac atcagttaag gagttcgcca tgaaacatga
    35881 ccattttgtt gttcaaagcc cggataaacc tgcgcaacag ttgttactgc ttttccacgg
    35941 tgtcggggat aacccggtgg caatggggga aataggtagc tggtttgcac cgttgttccc
    36001 ggatgcgctg gtggtgagtg tcggtggcgc ggagccgagc ggtaatccgg cggggcgtca
    36061 gtggttttcg gtgcagggta ttacggaaga taatcgccag gcgcgtgtgg atgccatcat
    36121 gccgacgttt attgaaacgg tgcgctactg gcagaaacag agcggagtag gggctaacgc
    36181 cacggcgcta atcggtttct ctcaaggcgc gattatggcg ctggagagca ttaaagccga
    36241 accgggcctt gcttcacgcg tcattgcttt taacggacgc tatgcgagcc tgccggaaac
    36301 cgcgtctaca gccacgacaa ttcacttgat tcatggcggt gaagatccgg ttatcgatct
    36361 ggcgcatgca gttgcggcgc aggaagcgtt aatcagtgcc ggtggtgacg tgacgctgga
    36421 tatcgtggag gatttaggcc atgcgatcga caatcgcagt atgcaatttg ccctggatca
    36481 cttgcgttat accattccga aacattattt cgacgaagcg ctaagcggcg gtaagcctgg
    36541 cgatgatgat gtgattgaaa tgatgtaata atagccgggt aggcctgata agaccgtatc
    36601 aggcatcatg gccggatgcg gcgttaacgc cttatccggc cacttagctt acttcttcgg
    36661 ttgatccttt ttcggccagt cgtcgtcatc gtcccactta tcgttgaaat cacgatgcgg
    36721 gggaagctcc ggcttgttgg cgagaaattt cttatggtcg acgcgcttga gatctttaat
    36781 cacgttcagc aggacgccta ccaaaaagac aatcaccaga atccaccaat atttagccag
    36841 ccagtccatg cttatttcct cttgcagaac cactcatcac gcgacgagct gttccatgat
    36901 acgttgatac atacgggcaa gtagctgcag gtcggcagcg ttcacacatt cattaatttt
    36961 atgaatagtg gcattgaccg gcccgagttc caccacctgc gcccccatgc gggcaataaa
    37021 gcgcccgtcg gacgttccgc ctgtggtcag tagctgcggt ttaatttcat tatagtgctc
    37081 aaccgcgtta acgaccgcat ccaccagttt accgcgcgcg gtcaaaaatg gctgcccgga
    37141 aagccaccaa tccaccgtat agcgcagttg atgtttttca agcagggcaa gcacctgcgc
    37201 tttgatcatc tcatcagtca gttcggtgct gaagcggaag ttaaactgca caaacagttc
    37261 acccggaata acgttgttac tgcccgttcc cgcctgaata ttggcaatct gcatactggt
    37321 cgccgggaag aattcattgc cctgatccca ctcaatagcc actaattcat taaggaaagg
    37381 tgctgcgcga tgtaccggat tgtcagccag atgtgggtag gcaacatgcc cctgaacgcc
    37441 atgaatggtt aggttgcagg ttaacgatcc acgacgacca tttttcacca catcacctac
    37501 cacttcgata ctcgacggtt cgccgaccag gcagtaatcg agacgctcat tacgtgccat
    37561 taacgcttcg acggctttta ccgtaccatt gtgggcactg gcttcttcat cagaggtgat
    37621 cagaaatgcc agtcgccctg tatggttggg atgttgtgcg acaaaacgtt ctgcagctac
    37681 caccatcgcc gccagcgagc ctttcatatc tgccgcaccg cgcccgaata acatgccgtc
    37741 acgaatggtt ggttcaaacg gcggattgat ccaacgatcg gcgtcgccag gcggcaccac
    37801 gtcggtatgc ccggcaaagg ccaatgtttc accttgccca cgccatgccc aaaaattctg
    37861 cgtatcggca aagtccatgc gttcaacggt aaaaccgatc gcctgcaaac gttcaatcaa
    37921 caaagcctgg catcctgcgt catcaggact cagggaaggg cggcgaataa gctgttgtgt
    37981 cagctcaata accgggcacg acatagacta cacctcatgg aaaaatttct gataactgga
    38041 atcactgaaa cccagcagca taggcttacc aggcgcgcag agcaatggac gtttgataat
    38101 tgcaggcatt tcagtcatta atgccgccgc agaggccgca tcggtgatta tattgcgggt
    38161 ggtttcgtcc agtttacgcc aggttgtacc acgggtgttg agtaacgctt cccagcctaa
    38221 ttcgttgata aaaccgttca ataattcgct gtccagccca tcgacgcggt aatcatgaaa
    38281 acgatagtcg atgttattgg cttccagcca acggcgagcc tttttaatgg tgtcacaatt
    38341 tttgatgccg taaagtgtaa ccataatgaa tcctttttcg atttatgaga ataaatactc
    38401 atttaagagt gaaaacattt tgcctgcaaa tgattatcag gaaatataac attatcgaag
    38461 tattcaaatt aac
//
LOCUS       DAZKIP010000030        38213 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZKIP010000030 DAZKIP010000000
VERSION     DAZKIP010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38213
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.030"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            139..1722
                     /gene="yegH"
                     /locus_tag="ACHOUK_001816"
     CDS             139..1722
                     /gene="yegH"
                     /locus_tag="ACHOUK_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFP9897280.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            1797..2135
                     /locus_tag="ACHOUK_001817"
     CDS             1797..2135
                     /locus_tag="ACHOUK_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9897281.1"
                     /translation="MRKKLAFLDTSLDDLRAFPESSRQEIGYQLDRIQQGLNPYDWKP
                     FSTIGPGVREIRTRDADGIYRVMYVAKFEEAVYVLHCFQKKTQTTSQSDIDLAKRRYK
                     ELVQERKNEN"
     gene            2125..2415
                     /locus_tag="ACHOUK_001818"
     CDS             2125..2415
                     /locus_tag="ACHOUK_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9897282.1"
                     /translation="MKIETFDSVWDAVSDTPEQAENMRIRAELVTIINNWIEQQGFSQ
                     AQAASALGVTQPRISELARGKIQIFSIDKLITMMAHAGLHIQRIEVQYPHAA"
     gene            complement(2468..4321)
                     /gene="asmA"
                     /locus_tag="ACHOUK_001819"
     CDS             complement(2468..4321)
                     /gene="asmA"
                     /locus_tag="ACHOUK_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFP9897283.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESRKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMADTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(4343..4924)
                     /gene="dcd"
                     /locus_tag="ACHOUK_001820"
     CDS             complement(4343..4924)
                     /gene="dcd"
                     /locus_tag="ACHOUK_001820"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFP9897284.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDESEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(5016..5657)
                     /gene="udk"
                     /locus_tag="ACHOUK_001821"
     CDS             complement(5016..5657)
                     /gene="udk"
                     /locus_tag="ACHOUK_001821"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFP9897285.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5975..9293
                     /gene="dgcE"
                     /locus_tag="ACHOUK_001822"
                     /pseudo
     CDS             5975..9293
                     /gene="dgcE"
                     /locus_tag="ACHOUK_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(9394..10242)
                     /gene="alkA"
                     /locus_tag="ACHOUK_001823"
     CDS             complement(9394..10242)
                     /gene="alkA"
                     /locus_tag="ACHOUK_001823"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFP9897286.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSADLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            10376..11728
                     /gene="yegD"
                     /locus_tag="ACHOUK_001824"
     CDS             10376..11728
                     /gene="yegD"
                     /locus_tag="ACHOUK_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP9897287.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARIQEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(11741..13681)
                     /gene="yegI"
                     /locus_tag="ACHOUK_001825"
     CDS             complement(11741..13681)
                     /gene="yegI"
                     /locus_tag="ACHOUK_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFP9897288.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLPSFVGFERTANHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDIAHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATARPTAKAWVAALDLLRQQLKKCTVSAMHVYPGHLTDCPWCALDNQGVIY
                     FIDLGEEVITTSGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKVYKAEIQQRREAFNRA
                     KMDYDHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDAASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAVTPAERQAVMAKMAAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(13678..14439)
                     /gene="pphC"
                     /locus_tag="ACHOUK_001826"
     CDS             complement(13678..14439)
                     /gene="pphC"
                     /locus_tag="ACHOUK_001826"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HFP9897289.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAINEAMAYVSQKVQHGEFGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDVVSRLETFTSTERAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLAAATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLTLALWLP"
     gene            complement(14436..15095)
                     /gene="yegL"
                     /locus_tag="ACHOUK_001827"
     CDS             complement(14436..15095)
                     /gene="yegL"
                     /locus_tag="ACHOUK_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFP9897290.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMNGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADIKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(15316..15375)
                     /gene="ibsA"
                     /locus_tag="ACHOUK_001828"
     CDS             complement(15316..15375)
                     /gene="ibsA"
                     /locus_tag="ACHOUK_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFP9897291.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(15647..15703)
                     /locus_tag="ACHOUK_001829"
     CDS             complement(15647..15703)
                     /locus_tag="ACHOUK_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFP9897292.1"
                     /translation="MMKWIIIVVLLVISFPAY"
     gene            complement(15975..16031)
                     /locus_tag="ACHOUK_001830"
     CDS             complement(15975..16031)
                     /locus_tag="ACHOUK_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFP9897293.1"
                     /translation="MMKILIIVVLLVISYPAY"
     gene            16311..17558
                     /gene="mdtA"
                     /locus_tag="ACHOUK_001831"
     CDS             16311..17558
                     /gene="mdtA"
                     /locus_tag="ACHOUK_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFP9897294.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLTNARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLLFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            17558..20680
                     /gene="mdtB"
                     /locus_tag="ACHOUK_001832"
     CDS             17558..20680
                     /gene="mdtB"
                     /locus_tag="ACHOUK_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFP9897295.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYSGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQISDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            20681..23758
                     /gene="mdtC"
                     /locus_tag="ACHOUK_001833"
     CDS             20681..23758
                     /gene="mdtC"
                     /locus_tag="ACHOUK_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFP9897296.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGMVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKSKQTVTE"
     gene            23759..25174
                     /gene="mdtD"
                     /locus_tag="ACHOUK_001834"
     CDS             23759..25174
                     /gene="mdtD"
                     /locus_tag="ACHOUK_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9897297.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAITGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSSTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            25171..26574
                     /gene="baeS"
                     /locus_tag="ACHOUK_001835"
     CDS             25171..26574
                     /gene="baeS"
                     /locus_tag="ACHOUK_001835"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFP9897298.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            26571..27293
                     /gene="baeR"
                     /locus_tag="ACHOUK_001836"
     CDS             26571..27293
                     /gene="baeR"
                     /locus_tag="ACHOUK_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFP9897299.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLSYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            27484..27816
                     /gene="yegP"
                     /locus_tag="ACHOUK_001837"
     CDS             27484..27816
                     /gene="yegP"
                     /locus_tag="ACHOUK_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFP9897300.1"
                     /translation="MAGWFELSKSSDSQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            27964..29325
                     /gene="yegQ"
                     /locus_tag="ACHOUK_001838"
     CDS             27964..29325
                     /gene="yegQ"
                     /locus_tag="ACHOUK_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFP9897301.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDNVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(29599..29817)
                     /gene="ogrK"
                     /locus_tag="ACHOUK_001839"
     CDS             complement(29599..29817)
                     /gene="ogrK"
                     /locus_tag="ACHOUK_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage transcriptional regulator OgrK"
                     /protein_id="HFP9897302.1"
                     /translation="MFHCPLCQHAAHARTSRYITDTTKERYHQCQNVNCSATFITYES
                     VQRYIVKPGEVHAVRPHPLPSGQQIMWM"
     gene            complement(29899..31062)
                     /locus_tag="ACHOUK_001840"
     CDS             complement(29899..31062)
                     /locus_tag="ACHOUK_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFP9897303.1"
                     /translation="MLDALTFDAGSTLTPDYMLMLDSRDITGNISDRLMSMTLTDNRG
                     FEADQLDIELNDADGQVGLPIRGAVLTVYIGWKGFALVCKGKFTVDEVEHRGAPDVVT
                     IRARSADFRGTLNSRREDSWHDTTLGAIVEAIASRNRLEASVAPSLAGIKIPHIDQSQ
                     ESDAKFLTRLAERNGGEVSVKMGKLLFLKAGQGVTASGKKIPQVTITRSDGDRHHFAI
                     ADRGAYTGVTAKWLHTKDPKPQKQKVKLKRKKKEKHLRALEHPKAKPVRQKKAPKVPE
                     AREGEYMAGEADNVFALTTVYATKAQAMRAAQAKWDKLQRGVAEFSIILATGRADIYT
                     ETPVRVSGFKRVIDEQDWTITKVTHFLNNSGFTTSLELEVRLSDVEYETEDDE"
     gene            complement(31062..31541)
                     /locus_tag="ACHOUK_001841"
     CDS             complement(31062..31541)
                     /locus_tag="ACHOUK_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9897304.1"
                     /translation="MMLALGMFVFMRQTLPHQTMQRESDYRWPSNSRIGKRDAYQFLG
                     VGEENMTLAGVLYPELTGGKLTMTTLRLMAEEGRAWPLLDGTGMIYGMYVISRVSETG
                     SIFFADGTPRKIDFTLSLTRVDEPLAALYGDIGKQAESLIGKAGSMATKFTGMTGAG"
     gene            complement(31556..34003)
                     /locus_tag="ACHOUK_001842"
     CDS             complement(31556..34003)
                     /locus_tag="ACHOUK_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9897305.1"
                     /translation="MSNNVKLQVLLRAVDQASRPFKSIRTASKSLSGDIRETQKSLRE
                     LNGQASRIEGFRKTSAQLAVTGHALEKARQEAEALATQFKNTERPTRAQAKVLESAKR
                     AAEDLQAKYNRLTDSVKRQQRELAAVGINTRNLAHDELGLKNRISETTAQLNRQRDAL
                     ARVSAQQAKLNAVKQRYQAGKELAGNMASVGAAGVGIAAAGTMAGVKLLMPGYEFAQK
                     NSELQAVLGVAKDSAEMAALRKQARQLGDNTAASADDAAGAQIIIAKAGGDVDAIQAA
                     TPVTLNMALANRRTMEENAALLMGMKSAFQLSNDKVAHIGDVLSMTMNKTAADFDGMS
                     DALTYAAPVAKNAGVSIEETAAMVGALHDAKITGSMAGTGSRAVLSRLQAPTGKAWDA
                     LKELGVKTSDSKGNTRPIFTILKEMQASFEKNRLGTAQQAEYMKTIFGEEASSAAAVL
                     MTAASTGKLDKLTAAFKASDGKTAELVNIMQDNLGGDFKEFQSAYEAVGTDLFDQQEG
                     ALRNLTQTATKYVLKLDGWIQKNKSLASTIGLIVGGALALIGIIGAIGLVAWPVITGI
                     NAIIAAAGAMGAIFTTVGSAVMTAIGAISWPVVAVVAAIVAGALLIRKYWEPVSAFFG
                     GVVEGLKAAFAPVGELFTPLKPLFDWLGEKLQAAWQWFKKLIAPVKATQDTLNRCRDT
                     GVMFGQALADALMLPLNAFNKLRSGIDWVLEKLGVINKESDTLDQTAARTHAATYGTG
                     GYIPATSSYAGYQAYQPVTAPAGRSYVDQSKNEYHISLTGGTAPGTQLDRQLQDALEK
                     YERDKRARARASMMHDG"
     gene            complement(33996..34151)
                     /locus_tag="ACHOUK_001843"
     CDS             complement(33996..34151)
                     /locus_tag="ACHOUK_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFP9897306.1"
                     /translation="MTFPKNLSVDDLMADVAVVFHWPPSELYPMSLTELITWREKALR
                     RSGNTNE"
     gene            complement(34148..34423)
                     /locus_tag="ACHOUK_001844"
     CDS             complement(34148..34423)
                     /locus_tag="ACHOUK_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFP9897307.1"
                     /translation="MNKENVITLDNPVKRGEQVIEQVTLMKPNAGTLRGVSLAAVANS
                     EVDALIKVLPRMTAPMLTEQEVAALELPDLVALAGKVVGFLSPNSVQ"
     gene            complement(34480..34998)
                     /locus_tag="ACHOUK_001845"
     CDS             complement(34480..34998)
                     /locus_tag="ACHOUK_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFP9897308.1"
                     /translation="MAMPRKLKLMNVFLNGYSYQGVAKSVTLPKLTRKLENYRGAGMN
                     GSAPVDLGLDDDALSMEWSLGGFPDSVIWELYAATGVDAVPIRFAGSYQRDDTGETVA
                     VEVVMRGRQKEIDTGEGKQGEDTESKISVVCTYFRLTMDGKELVEIDTINMIEKVNGV
                     DRLEQHRRNIGL"
     gene            complement(35011..36201)
                     /locus_tag="ACHOUK_001846"
     CDS             complement(35011..36201)
                     /locus_tag="ACHOUK_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HFP9897309.1"
                     /translation="MSDYHHGVQVLEINDGTRVISTVSTAIVGMVCTASDADAETFPL
                     NKPVLITNVQSAIAKAGKKGTLAASLQAIADQSKPVTVVVRVEDGTGDDEETKLAQTV
                     SNIIGTTDENGQYTGLKALLAAESVTGVKPRILGVPGLDTKEVAVALASVCQKLRAFG
                     YISAWGCKTISEVKAYRQNFSQRELMVIWPDFLAWDTVSSTTATAYATARALGLRAKI
                     DQEQGWHKTLSNVGVNGVNGISASVFWDLQESGTDADLLNESGVTTLIRRDGFRFWGN
                     RTCTDDPLFLFENYTRTAQVLADTMAEAHMWAVDKPITATLIRDIVDGINAKFRELKT
                     NGYIVDATCWFSEESSDAETLKAGKLYIDYDYTPVPPLENLTLRQRITDKYLANLVTS
                     VNSN"
     gene            complement(36261..36854)
                     /locus_tag="ACHOUK_001847"
     CDS             complement(36261..36854)
                     /locus_tag="ACHOUK_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9897310.1"
                     /translation="MLIGYVRVSTNDQNTELQRNALECAGCELIFEDKISGTKSDRPG
                     LKKLLRTLSAGDTLVVWKLDRLGRSMRHLVILVEELRERGVNFRSLTDAIDTSTPMGR
                     FFFHVMGALAEMERELIVERTRAGLEAARAKGRIGGRRPKLTTSEWEQVGRLLAAGES
                     RQRVALIFDIGLSTLYKKFPSSATKNKLCHPLANRDK"
     gene            36882..37415
                     /locus_tag="ACHOUK_001848"
     CDS             36882..37415
                     /locus_tag="ACHOUK_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000983068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9897311.1"
                     /translation="MMPVTRYFCIFINVGLGEGSALPVGVPVPWPSATPPTGWLKCNG
                     AAFSAEEYPELAKVYPTNKLPDLRGEFIRGWDDGRGVDNGRGLLTLQDGAIVSHNHYW
                     GIWTSRTNDQTLESFTGTTILKQITPLSPAINFDNYPIPNPAITEGGVVAATTKPAGA
                     NETRPRNVAFNYIVRAA"
     gene            37415..38017
                     /locus_tag="ACHOUK_001849"
     CDS             37415..38017
                     /locus_tag="ACHOUK_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP9897312.1"
                     /translation="MNNAELNSELIATMAGEITVYNFDVMSREYISASTEYLAIGVGI
                     PAYSCLDAPGAYKAGYAICRSADFNSWEYVPDHRGEIVFSTETGESKEITAPGDYPDN
                     TTTIAPLTPYDKWDGEKWVTDTEAQHSAAVEAAETQRQSLIDTAMASISLIQLKLQAG
                     RKLTQAETNRLNTVLDYIDAVTATDTSTAPDVIWPELPEA"
     gene            complement(37989..>38213)
                     /locus_tag="ACHOUK_001850"
     CDS             complement(37989..>38213)
                     /locus_tag="ACHOUK_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP9897313.1"
                     /translation="LIEITESERQLLINQANEYMNSKQWPGKAAIGRLKGEELAQYNL
                     WLDYLDALELVDTASAPDIEWPTPPAVQAR"
BASE COUNT         8657 a        10341 c        10051 g         9164 t
ORIGIN      
        1 tttaattcag ggataatgtg aggttattac cctcaaataa tatgagataa atagtgctgc
       61 aacattgcat ttttgccccg atttatccat gatcgaattg tgacatttgt catacaacga
      121 ataggttttg tacttactat ggaatggatt gccgatccgt ctatctgggc cgggttaatc
      181 acgctgattg tgatcgaact ggtcctcggc attgataacc tggtctttat tgccatcctc
      241 gccgaaaaac taccgccgaa gcagcgcgac cgcgcacggg ttaccgggct gttgctggcg
      301 atgttaatgc gcctgttact gctggcgtca atctcctggc tggtcaccct gactcaaccg
      361 ctgttcagct tccgctcgtt tacctttagc gcccgcgact taatcatgct gtttggtggt
      421 ttcttcctgt tgttcaaagc cacgatggag ctgaacgaac ggctggaagg gaaagacagc
      481 aataatccca cacaacgcaa aggtgcgaag ttctgggggg tggtgacaca aattgtggta
      541 ctggacgcca tcttctcact cgactcagtg attaccgccg tcgggatggt cgaccattta
      601 ctggtcatga tggccgccgt ggttatcgct atcagcctga tgttgatggc cagcaagccg
      661 ttaacgcaat ttgttaacag tcacccgacc atcgttattc tctgcttaag cttcctgttg
      721 atgattggct ttagcctggt ggcagaaggt ttcggcttcg tcattccgaa aggctacctg
      781 tacgctgcca ttggtttctc ggtgatgatc gaggcgctca atcagttggc tatctttaac
      841 cggcgacgtt ttctttccgc taaccagaca ctgcgccagc ggactaccga agcggtaatg
      901 cgcctgctta gcgggcaaaa agaggatgcg gaactggacg ccgaaaccgc gtccatgctg
      961 gtggatcatg gtaaccagca gatctttaat ccgcaggaac ggcggatgat tgagcgggta
     1021 cttaatctta accagcgtac cgtcagcagc attatgacgt cgcgccacga tattgagcat
     1081 atcgatctca acgcgccgga agacgagatc cgccagttgc tggagcgaaa tcagcatacg
     1141 cggctggtgg ttactgatgg cgatgacgcg gaagatttgc tcggtgttgt tcacgttatc
     1201 gacctgttac aacaatcact gcgcggcgaa ccgctcaacc tgcgggtgtt aattcgccag
     1261 ccgctggtgt tcccggaaac cttgccgttg ttacctgccc tggagcagtt ccgtaatgcc
     1321 cgcacacact ttgcttttgt ggtggatgag tttggctcgg tggaagggat tgtgacatta
     1381 agtgacgtca ctgaaaccat tgccggtaac ttaccgaacg aagtggaaga gatcgacgcc
     1441 cgccatgata ttcagaagaa tgcggacggt tcctggacgg cgaatggtca tatgccgctg
     1501 gaagatctgg tgcaatatgt gccgctgccg ctggatgaaa aacgtgaata tcacaccatt
     1561 gccgggctat tgatggaata tttgcagcgt attccaaaac ctggcgaaga agttcaggtg
     1621 ggggattatt tgcttaaaac gttgcaggtg gaaagccatc gcgtgcagaa ggtgcagatt
     1681 ataccgctgc gtaaggatgg cgagatggag tacgaggtgt gataaaacat caaatgcgca
     1741 ggttcaaact gcgcatttga tttatacaaa atttagtata atctaaggat ggagtaatga
     1801 gaaaaaagct ggcttttctt gataccagtc ttgacgattt acgcgcattt ccagaatcgt
     1861 ctcgacagga aattggctat caactcgaca ggatccaaca agggttaaac ccatacgact
     1921 ggaaaccttt ttcaactatc ggccctggtg tgcgagagat tcgtactcgc gatgctgatg
     1981 ggatctatcg cgtcatgtat gtcgcaaaat ttgaagaagc ggtttacgta ttgcattgtt
     2041 ttcaaaagaa aacacagact accagtcaat cggatattga tttagccaaa cgacgctata
     2101 aagaacttgt tcaggagcgg aaaaatgaaa attgaaacct ttgatagtgt gtgggatgcg
     2161 gtgagtgaca cccctgaaca agcggaaaat atgcggatcc gtgccgagct tgtaacgatc
     2221 attaataact ggattgaaca acaagggttt agccaggctc aggcagcttc tgctttaggg
     2281 gtcactcaac ctcgcatctc tgaacttgcc agaggtaaaa tccagatctt tagtatcgat
     2341 aaattgatca ccatgatggc tcatgcagga ctacatattc aacgtattga agttcaatat
     2401 ccacatgctg cttagtgcta taaatacaga tggcgacaaa ttgatgccgc catctatata
     2461 acttgtctta catcttctcc agcaacttct tcacatcttt gccattgcgg gaatctttat
     2521 tccgctcggc ccagtcattc aggcgacgtt tcgcttcgtc ctgtagatgt ttgcgcagta
     2581 actgatccac ttgcagactg taattgagtt gctgccagtt gccataaacc cgcagcggta
     2641 ctggcgtttc tttgaggaaa tcaatcagtt tgctttcccc gttccagcca cccacgaccc
     2701 gaatatcaaa ctgggtgtcg caggtttgat ctgccagatt caacatgcct tcccctgtaa
     2761 gcgccagcac aggcgattga ccttgcatgt cgtttaacgt cacgacgcca tccttcaacg
     2821 tcaaatcggt ggtaaagcgg tcaagacgcg ttacgttatc gaagttttca gcggccttca
     2881 catcaccacc attacgttct accgcttgct gaatcatctg ctggaagttc atcccttcca
     2941 tgcgcgtatc ggccatttcg acatgtgcct gtccttgcca gttgtggcgg aatgcgtcgg
     3001 catctatgtc agcaccggag aaatcaccag ccaatgacat ttttccggtc aacgaaatcg
     3061 gatagttaaa cgccttcaga atggtgccaa tctcaacgtt ttccagccgt ggctggaagt
     3121 ttatccgcgg atttattgat gtcgcgtcca gcgtgcccgg cagtgaaacc tgtccaccgt
     3181 taagcttgcc ctgtagttga gtaatttcca gcaaacccga cttgttggtc atttgcgtgg
     3241 caacatctgt aaaattcatt ccgcgccagc gcacgttact ggcctgcaac aaaatatcag
     3301 ccgtaaagcc ttgcagtccc tgataggccg gttcatcaat acgcgaagaa ataaccgggc
     3361 gcggcaacgt tgattggctc tgcccctgct gcgcggcacc gttttcacca ttcgcggttt
     3421 cattaagcgg gatgaggttg tcaagattca gttgcgggaa ttgcagcctc agctgccatt
     3481 ccggtttctc tgtcagcgtg acctgtgctt gcccgctcag cgtactatca ttggcggtca
     3541 aactaatttg gttaaatgaa aggcgtttac gtgactcctg ccactgggcc tggaaactcc
     3601 cctgcccctg aataccttgt tttggtaaat cggcaccctg caactgccag ttaatttgtt
     3661 caatagccgc cgttaaatca tgcggataat cagaagcatc taccgtaccg ttaagggata
     3721 tcgtgagatc gcgctgatcg cgattaaccc gcccggagaa ctcaaatgag ccacgatgtt
     3781 ggggatcttg ttccatttgc aggcggatat tgcggattgt cacctgctcg tcatcttcat
     3841 gctggaacac cagcacgctg tccgccacct taagactgga tatatcaaac gaccatccgc
     3901 gatcgtctga cagatccggc aaggtattgt cgcgcggtgc aaccggagcg tcttcactgc
     3961 gcaccgcttc cgtctgcggc gtcagttgga tcactgcccc ttttagcatc acctgcttaa
     4021 cgctcagttg atgactcagt agtggtaaaa gcgccacgtc caggcgcatg ttgtcggcgc
     4081 gaaccagtgg ctggctcgcg ccctgggcgg tgagagacat tcgcccggag aggatactaa
     4141 gctgcggcca gacgtgccaa cgcagtggcc cgtcgagctg caattgataa ccgctacgtg
     4201 cagcaacttg cttgaccata tagtcgcgga aatcattcgg attcaccagc aacactaacg
     4261 cagataaccc ggccaccagc acgaccagga gtatcatcag cgtcgtcaga aatcgtctca
     4321 tggtatcctc aatgggcctg aattagtctt tatcgattcg gctggctacc gcgccctgct
     4381 ggttgcgata cttcgcatct tcacggcggt tgtaaggtcg cgccgccggg ccggaaagcg
     4441 gctcaaagct cagcgcacca attaacatgc ccggacgcag cgccagcggc agcttaccgg
     4501 agttgtagaa ctccagcaca atgcaaccag accagcccgg atcgatacgg tgcgcggtga
     4561 cgtgcaccat cagccccaga cgcgccagtg aggaacgccc gtccagccag cccaccagat
     4621 cggctggcag cgtcaccgac tccagcgtca ccgccagcgc cagctctcct gggtgaagat
     4681 agaacgcctc gctttcatcg agaacgatct catcgctcat cacgcggtca agcgcggcgc
     4741 tcacttcatc tttgggaccg ctcagatcga taaacgctgc cgtgtgacca cggaaggtac
     4801 gaaatttatt gcccaggcgt acatccaccg tcgcgccgtt aatacgctcc actggcggac
     4861 gtgggttgat cgacaaacgg ccttcatcaa gccaggcttc aatatctcgg tcacacagac
     4921 gcatggcatt ttctcctttc gcgcatcact cccttaacgc caattacgtc aagggcatac
     4981 caggttatca ctgaacggta cacaatttat caagcttatt caaagaactg acttattttc
     5041 gctttcaata tatcgatcgc gatgcggttt ttcccgccgc gcggcacgat aatgtccgca
     5101 tattgtttag aaggctcaat gaattgcagg aacatcgggc gcacggtttt ttgatattgc
     5161 gccatcacgg aatccattga acgcccacgc tcgttaacgt cacgcttgat gcggcgcatc
     5221 aggcagatat ccagcggggt atcaacgaaa atggagaagt taagttcgtc acgcaaacgt
     5281 gcatccgtca gcaacaaaat gccttcgaga atgatgacct tcttcggctc aaccgtcacc
     5341 gtttctttca tacgcgtatg ttcaacatag ctgtaaaccg gcaggtcaat tgccgagccg
     5401 cgtttcaacg cttgtaaatg ctcaagcagc aggctgtgat ccatcgcgct gggatggtcg
     5461 tagttggtct taacgcgttc ttccatcgac agatggcttt gatctttgta atagcagtct
     5521 tcgggaatta cgccgatgtg ttcatcaccg acttgctcac gtaattcacg ataaagggta
     5581 ctggcaataa gactcttgcc ggaagccgat gcgccagcga taccgataat gacgcactga
     5641 tgagactgat cagtcatata tttagcgacc tgattaacct ggatgttagg aagggggcga
     5701 cgaagcgcca aacgcggcaa ttatagggat ttcatccgcc tgataccagt cgaatagcgt
     5761 tgccgcgcgc tcagagttaa ttgttgacga agaattcccg gtggcaaatt acgttgatca
     5821 gttttataca aggtaaaaat tgttatacgc agttgcgcaa attatccgcc tttacgtcac
     5881 tttatgagca attcgcatat aaaatgtaaa acttttgtac tagcataaac acagaaacga
     5941 atactggcga cctggccttg cggataaagc ggtaatgagc aaacaatcac agcatgtatt
     6001 aattgccctg ccccacccgc tgcttcacct ggtcagttta ggtttagtct cgtttatctt
     6061 cacccttttc tcgcttgagc tttcgcagtt tggcacccaa ctcgccccac tgtggttccc
     6121 gacgtccatc atgatggtgg cgttttatcg ccatgccggg cgcatgtggc cagggattgc
     6181 gctgagctgc tcgctgggaa atatcgccgc atccatcctg cttttttcca ccagctcgct
     6241 gaacatgacc tgcacgacca tcaatattgt tgaagccgtg gtcggggcag tgctgctacg
     6301 taaattgctg ccgtggtata accccttgca aaatctggct gactggctgc gtctggcact
     6361 cggcagcgcc attgttccac ctctgttggg gggtgttctg gttgtcctgc tgacgcccgg
     6421 agacgatcct catcagggca tttttgatat gggtactgtc agaatccatc ggcgctctgg
     6481 cactggtgcc gctgggattg ttatttaaac cacactatct gctgcgccat cgcaacccac
     6541 ggttgctttt tgagtcgctg ctcacgttag ccatcacact gacgttaagc tggctttcga
     6601 tgctgtatct gccgtggcct tttactttca ttattgtgct gttgatgtgg agcgccgtgc
     6661 gcctgccacg aatggaagcc tttttgatct tccttaccac ggtgatgatg gtgtcgctga
     6721 tgatggccgc ggatccctcc ctgcttgcta cgccgcgtac gtacctgatg agccatatgc
     6781 cgtggctacc gtttttgctg atcctgctgc ccgccaacat catgacgatg gtgatgtatg
     6841 cctttcgtgc ggaacgcaaa cacatttccg aaagcgaaac ccgttttcgg aacgccatgg
     6901 aatattccgc catcggcatg gcattagtgg gcaccgaggg acaatggctg caatccaaca
     6961 aagcactctg ccagtttctc ggttacagtc aggaagagct gcgcggactc acctttcagc
     7021 aactgacctg gccggaggat ctcaataaag atctccaaca ggttgaaaag ctgataagcg
     7081 gtgaaataaa cacctattca atggaaaaac gttactacaa ccgcaatggc gatgttgtct
     7141 gggcgttgct tgccgtctct ctggtgcgcc acacggatgg cacgccgctc tattttatcg
     7201 ctcagattga agacattaac gagctaaaac gcaccgaaca ggtgaatcag caactgatgg
     7261 aacgcatcac gctggctaac gaagcgggcg ggattggcat ctgggagtgg gaactgaagc
     7321 cgaatatttt tagctgggat aagcggatgt tcgagctgta tgaaattcct ccgcatatca
     7381 aaccgaactg gcaggtgtgg tacgagtgcg tgctgccgga agatcgccaa cacgccgaaa
     7441 aagtgattcg tgattcgttg caatcacgct cgccctttaa actggaattt cgcattgccg
     7501 tgaaagatgg catccgccat atccgcgccc tcgccaaccg ggtactgaat aaagaaggcg
     7561 aagtcgaacg cctgctcggc attaatatgg atatgactga ggttaaacag cttaacgagg
     7621 cattgtttca ggaaaaagag cgcctgcaca ttacgctgga ctccatcggc gaagccgtgg
     7681 tctgtattga tatggcgatg aaaattacct ttatgaatcc agtggcggag aagatgagcg
     7741 gctggacgca ggaagaagcg ttaggtgttc cgctcctgac ggtgttgcat attacttttg
     7801 gcgacaacgg accattaatg gagaacattt acagtgccga cacctcacgt tccgcgatcg
     7861 aacaagatgt ggtgttgcac tgtcggagcg gcggcagcta cgacgtgcat tacagtatta
     7921 cgccgttaag tactctggac ggcagcaata taggctcggt actggtgatt caggacgtta
     7981 ccgaatcacg caaaatgctg cgccagctga gctacagcgc ctcccatgat gcactgacgc
     8041 atctcgccaa ccgcgccagt tttgagaaac aactgcgtat cctgctgcaa acggtaaaca
     8101 gtacgcatca gcgacatgca ctggtgttta tcgatcttga tcgctttaaa gcggtgaatg
     8161 acagcgccgg gcatgcggcg ggcgacgctt tgctgcgcga actggcgtcg ttgatgctga
     8221 gtatgctgcg ctccagtgac gtgctggcgc ggctcggcgg tgatgaattt ggtctgctgc
     8281 tgccagactg caatgttgaa agcgctcgtt ttatcgctac acgtattatc agtgctgtga
     8341 atgactatca ctttatatgg gaaggccggg tacatcgggt aggtgccagc gccgggatta
     8401 ccttgatcga tgacaacaat catcaggcgg cagaagtgat gtcgcaggct gatatcgcct
     8461 gttatgcctc caaaaatggt ggccggggcc gggtgacggt ttacgaaccg cagcaagctg
     8521 ccgcacatag cgaacgggcg gcgatgtcgc ttgatgaaca gtggcggatg attaaagaga
     8581 atcagttgat gatgctcgcc cacggtgtcg cttcgccacg gatcccggaa gcgcgtaatt
     8641 tgtggctgat ttcacttaag ctctggagtt gcgaaggcga gattattgat gaacaaacat
     8701 ttcgtcgtag cttcagcgat ccggcgctta gccatgctct tgaccgccgg gtattccacg
     8761 aatttctcca gcaggccgca aaagcggttg ccagtaaagg cataagcatc gccctccccc
     8821 tttccgttgc cggtttgagt agcgccacgc tggtgaatga tctgcttgag cagctggaaa
     8881 atagccctct accaccacgg ttattacatc tgattattcc ggcagaagcg attttcgatc
     8941 acgcagaaag cgtgcaaaaa ctgcggctgg ggggatgccg gatcgtattc agtcaggtgg
     9001 gccgcgatct gcaaatcttc aactcgctga aagcgaatat ggcagattac ctgctacttg
     9061 atggtgagtt atgcgccaac gtgcagggta atttgatgga tgagatgctg attacgatta
     9121 ttcaggggca cgctcagcga ctcgggatga aaaccatcgc cgggccagtc gttttaccct
     9181 tagtgatgga tacgctttct ggcatcggcg tcgatctgat ttatggtgag gtgattgccg
     9241 atgcccaacc gctggatttg ctgatgaata gtagttattt cgcgattaac tgagggagcg
     9301 tttgcccatt gcctgatgcg acgctaacgc gtcttatcag gcctacaaat cgctcattcc
     9361 gcaaggcgct cgcaccgcat ccggcgacca acgtcatgct tcgtctggtt gccagccttc
     9421 cgtataccag atatgcaaca gcgcataaga acgccagggc ttccagcgct cggcataacg
     9481 gcggatttgc gccggtgtca ttcccggaaa tcgctgttta atcagataat catccggcag
     9541 gaaaacatct ttcgcctgcc agccacgcaa agcaaaataa ttcgccgtcc agcgcccgat
     9601 acccggaaaa gtttgcagcg ttttcatcgc ctgctccaca tcgcccggta ttgtcattgg
     9661 taaggtgccc tccagcgccg catttgccag atgaatcagc gcctctgccc gtttcaacgg
     9721 catacctaac gcttttaatg cctgcgggtc tgctgctgcc agccgctgag gcgtggggaa
     9781 gcagatatac tccggaaaat catccagccg ttcgccataa agctgtacca ctttggcagt
     9841 caattttgcc gccatcgcca cgctcaccag ttggcctaaa atcgcccgca cgccttgctc
     9901 aaaagcatca acactgccgg gtaaacgcaa tccgggccgc gccgcgccta acttgcccaa
     9961 cgcaccgtta acaatctgcg ggttacattg cagatcaagc aggcggctca ttttcgccag
    10021 acactcagcg gcaacgggtt ctaaatctgc acttaaattt atgtgcagag tatggcgggc
    10081 tatatccgga atagcagtca ccacgccgcg atattcgccc accgccagac tacgggcata
    10141 ataatcgtca gcgaccgttt ccacaccact cactgcacgg gcggcgagaa atcccaacat
    10201 ccacgaccag tcatacggcg gctgccagtt cagggtatac atcgcatctc cttattcatc
    10261 cgctttcagc ataaacgtta ttcagacgct gcgctttgct ttcatattcc ggttgtcgcg
    10321 acggcaacat ttcgctaaag tcacgcccct tcttcaccgg catggggatt atttcgtgtt
    10381 tattggtttt gattacggta cagcaaactg ttcagtagcg gtcatgcgtg acggtaaacc
    10441 gcacttgcta aaaatggaaa acgacagcac gctgctgcct tcaatgcttt gcgcgccaac
    10501 gcgtgaagcg gtaagcgaat ggctgtaccg ccatcatgat gttccggcag acgacgatga
    10561 aacgcaggca ctgttgcgtc gggcgattcg ttataatcgc gaagaagata tcgatgttac
    10621 ggcgaaaagc gtgcagttcg gtctttcctc actggcacag tacattgatg atccagaaga
    10681 agtgtggttt gtgaaatcac caaaatcgtt cctcggtgcc agcggcttaa aaccgcagca
    10741 ggtagcgctg tttgaggatc tggtctgcgc aatgatgttg cacattcgcc agcaggcgca
    10801 agcgcagctg ccagaagcga ttactcaggc ggtgattggt cgcccgatca acttccaggg
    10861 gctgggcggc gatgaagcaa acgcccaggc gcaagggatt ctggaacgcg cggcgaagcg
    10921 tgccggattc agggacgtgg tattccagta cgagccggta gcggctgggc tggattacga
    10981 agccacctta caggaagaaa aacgggtact ggtggtggat atcggcggtg gtacgactga
    11041 ctgttcattg ctgctgatgg ggccgcagtg gcgttcgcgt ctcgatcgtg aagccagcct
    11101 gctgggtcac agtggttgcc gtattggcgg taacgatctg gatatcgcac tggcgtttaa
    11161 aaacctgatg ccactgctgg gtatgggtgg cgaaaccgaa aaaggcatcg ccctgccgat
    11221 tctgccgtgg tggaatgcgg ttgccatcaa cgacgtacct gcgcagagtg atttctacag
    11281 tagtgccaac ggtcgtctgc ttaacgatct ggtacgcgat gcccgcgaac cggaaaaagt
    11341 ggccctgcta cagaaagtct ggcgtcagcg tttaagctat cgcctggtgc gtagcgcaga
    11401 agagtgcaaa attgcgcttt caagcgtagc ggaaacccgc gcctcactgc cgtttatcag
    11461 cgatgaactg gctacgctga ttagccagca agggctggaa agcgccctca gccagccact
    11521 ggcgcggatt caggaacagg tgcaactggc gctggataac gcccaggaaa aaccggacgt
    11581 tatctacctg accggcggaa gcgcccgttc tccgctgatt aaaaaagcgc tggcagaaca
    11641 gttgccgggc attccgattg caggcggcga tgactttggc tccgtcaccg ccgggctggc
    11701 acgctgggcg gaagtggtgt ttcgttaatt gaaaaatggc tcagcagcgg cttaaatctg
    11761 cctgcgcctg agccagtttt tctgccgcct gacgtaacgg ttccatcaac ggcatggtcc
    11821 gtgctggagc atgaaggcgg aatcgctgca actctgtcgc gccgacagtc aacgccgatt
    11881 ccagccgatg tcgtttggcg gccattttcg ccattaccgc ttgtctttct gcaggcgtta
    11941 ccgcttcgtt cggcctgaac acaaaacggc gttcacagct cgctttccag tcgataaccg
    12001 cctgggtcag atgatcacca aaccctttaa cttgcttaac cccacgacgg gtaacatccg
    12061 cggctgtttc aataccaaaa gaccgtaacg ccgctttacg cgcagggcca acgccgggaa
    12121 tagaggcagc atcaataaaa aatccctcca gaaacttctg cttctgccgt tcccttgcag
    12181 tgtcatgaag tgctgccaga gcgcgtttct cctcttccgg taacccgaga atttcgtcct
    12241 tcattttttc gagcatcgcc cgtttggcga taaaaccttc cagcccgccc aactgttgga
    12301 tctggctgac taaatggtca tagtccattt tcgcgcgatt aaatgcctca cggcgttgct
    12361 ggatttctgc tttataaact ttgcttgtca gactgccaat aatccagata gccgccagca
    12421 caggaaccaa aataatataa cgcggttctg cctgaaggcc gcaaagcaac agcgataacg
    12481 ctgacagtgc gatctcaatc agaatgatgt attcgcgccg taacaggcct aaaggaagcg
    12541 gcctgccagt cggttggaaa tgatcgggta atggcaattg caatgctggc ggtgctactg
    12601 acgccatcac catcgcccag actttcgcca gcacaaaatc accactggtg gtaatgacct
    12661 cttcgccgag atcaataaaa tagataacgc cttgattatc cagcgcacac cacgggcagt
    12721 cagtcaaatg accggggtaa acatgcattg ccgaaacggt acatttcttt aactgttggc
    12781 gtagtaaatc cagtgccgct acccacgctt ttgccgtcgg acgcgcggtt gccacgccac
    12841 tttccgtgaa tgcctgctga aacatggctt caacatcgcc cggtaacatc gacagcggaa
    12901 tcgatcgtgg cggcggtttt aaaccacgtc gctggttgtc tgacgcgtag gcataacgga
    12961 aatgggcaat atccgtctcc agcgcattac ccgcatcaga gataagcggc acaccagaat
    13021 aaggatgccg cccaccaaac aagacgtgaa aaatcagcaa cgcaaggcca aaattatcgt
    13081 gattcgcggt acgttcaaaa ccgacaaatg atggcaaggt ttgtagctct ggcggcgtaa
    13141 aatgcgacac gccgacttcg cataaatgca gtgtgccatt ggcgttaatc tgaaaggagt
    13201 cactatcgat caacaccact ttgctgtcgc gacctaccat aaagctgttc tggtttacgt
    13261 cccccacgac gtgcccgtgc tcgtgaaccg tagcaaaaga tgaagcaata ttgcgcgcaa
    13321 catagagtag aaaatcccac gcacaatgag gataactctg gcgacgatgt gccgggctat
    13381 agatcatatg aatcggttct ttaccagaaa cttttggcat cataaaaccg ataacttttc
    13441 cgcctcgtcc accgtgaagc gttgcctgcg gccaggcgac ataattcaac aactgcgcgt
    13501 cagctgtcgc agccataaag gcaagtttgt cctgttttaa ggcgggtggc ggcgtgtgat
    13561 aaatcttggc gacgctatcg acaaactcat tgatatcata aaccgcgcct tcgccacctt
    13621 tgcccagttc acggcctggc gtaacgcact caccagtagc agtatataaa gtgggtttca
    13681 tggtaaccac agcgccagtg tcaacgtttt atcatcatcg gtacgctcgt ttaccgctgg
    13741 gctcgacaaa aactgcttta acaattcagg taataaatcg agttgttcct gcgttgctgc
    13801 cgccagtcca ttgaaaaacg gggtaaagaa aggcacatgc ggagaattat ccagcatatt
    13861 taacgccagt cgctgaatac catccgtaaa tgctgcaact ttatgcgcac gctcagtgct
    13921 ggtgaaagtt tccagtctgg aaacgacatc ttcatcggta atgaaatgcg tcatattggc
    13981 atattcaccc accatcggcg taagcggtaa ttgcagaccg tgaccaaaat ccaccaccac
    14041 gccgccatcg ccaatctgca taatcaaggt gccgttagcc gatgaaatca atcccagaaa
    14101 cgtgcaggca aaatcgcgaa ctgctaattc tttagcttcc gcctcggcaa aaagtcgctg
    14161 acgaacggtc agcacaatat ccgtggcgag aatatcattg agtccaaatt caccgtgctg
    14221 cactttttgc gacacataag ccatcgcttc attgatggca agcatcgcgc cttcgccacc
    14281 ctgcgagaca ctgcctgcgc catcagcaag gaacatcacc agtaatggct gctggtcatt
    14341 tagccaggca acctgcatct ggcaggcatc ctgacaaggt aaatcagcgc taatgtgcga
    14401 cgttccaaca gcggatgcat aaaccagacg ccagctcaca ctgacgtcca gccttttggc
    14461 gcttccagca caacttccgt tcccggcgtg gagcgggaaa ccgaacgcag cgaactggat
    14521 aaccagctaa acagttcacg gaattgtaat ccctgcagtg gcaaaggctg acgaacgctg
    14581 atttgtgcca gagtcttaat atccgccccc tgaacaccga tggaaaagaa ggcaaacttc
    14641 ttatcttctt ccccctgaaa cactttgttg gcggcagcct gccactcatc ggttggtgca
    14701 ccatcggtga tcaggaaaat ccacgggcga taatacgaaa taccatttgc acgatactca
    14761 cgtttccgct cctctaccat atccagggct ttggtaatgg cggcacccat tggtgtatcc
    14821 ccctgggcaa acaggatggg cgggaaaaaa ttagcggcgc tggtaaaggg ttgttccaca
    14881 tgcaccgggc cgaacgtcac aataccaagt tccactcttt ttaaggccag cgggtcggca
    14941 agcagctcat cgcgaaaggt aaccaatccg gcgttaagtt cattaatggg tctaccgttc
    15001 atcgaaccag aaacatccag cagcaaaatg caggggcaac gtggttccgg gttactggca
    15061 aaatcgctgg tggcaaacgt gatttgttcg ctcataagaa atcctttctt agagaattat
    15121 tgaggtccgg tatcataccg attgcaataa tgaaataaga gcaaacgaag aaactttcag
    15181 aattaggccc ggcggcgcag aaaattttgc tattcgcctc gcaagaacgg agagccgtcg
    15241 attgacacta agggcggagt gacataattt caggagtgag ggttagggaa aggtttcccc
    15301 ctccccctgg tgttcttagt aagcctggaa gctaatcact aagagtatca ccagtatgat
    15361 gacgtgcttc atcataaccc ttttcttatt taaggcccct tcctcgggag gggctttccc
    15421 gtttcagcat cccgctgaaa tcgtcggctt acctcctttc gccatgcatg cagtctatcg
    15481 ctgacttgcg gataaaatag tttccagtgt gattattgcg taacgtctcg caaagaatat
    15541 ttgctggcag gatcgcagac tgcaaagcct gcagattgac aatcttatcg tgaaggcata
    15601 ctttcaggag tgagggtaga gcggggtttc ccccgccctg gtagtcttag taagccggga
    15661 agcttatgac taagagcacc acgatgatga tccacttcat catgaccctt tccttattta
    15721 aggccccttc ctcgggaggg gctttcccgt ttcagcgtcc cgctgaaatc gtcggcttac
    15781 ctcctttcgc cctgaattca gtctatcgct gacttgcgga taaaatagtt tcctctgtga
    15841 ttattgcgta acgtctcgca aagaatattt gctggcagga tcgcagactg caaagcctgc
    15901 ggattgacat tctgaacgtg aaggcatact ttcaggagtg agggtagagc ggggtttccc
    15961 ccgccctggt agtcttagta tgctgggtag ctaatcacta agagcaccac gatgatgagt
    16021 atcttcatca tgaccctttc cttatttatg gccccttcct cgggaggggg ctttcccgtt
    16081 tcagcgtccc gctgaaatcg tcggcttacc tcctttcgcc atgcaagcag tctatcgcta
    16141 acgcgtagat aaaatagttt cctgtgttat tactggatgc gtgctcgcaa atgtgcccgc
    16201 cattcagacg attcccgata gtgtttcata attcctccat ttttctccct tattggctgg
    16261 ctacactagt atcattccgc gaaacgtttc aggaagagaa actcttaacg atgaaaggca
    16321 gttataaatc ccgttgggta atcgtaatcg tggtggttat cgccgccatc gccgcattct
    16381 ggttctggca aggccgcaat gactcccaga gtgcagcccc aggggcgacg aaacaagcgc
    16441 agcaatcgcc agcgggtggt cgccgtggta tgcgttccgg cccattagcc ccggttcagg
    16501 cggcgaccgc cgtagaacag gcagttccgc gttacctcac cgggcttggc accattaccg
    16561 ctgctaatac tgttacggtg cgtagccgcg tggacggtca actgatggcg ttacatttcc
    16621 aggaaggcca gcaggtcaaa gctggcgatt tactggcaga aattgacccc agccagttca
    16681 aagttgcatt agcacaagcc cagggccaac tggcaaaaga taaagccacg cttaccaacg
    16741 cccgccgtga tctggcgcgt tatcagcaac tggcaaaaac caatctcgtc tcccgccagg
    16801 agctggatgc ccaacaggcg ctggtcagtg aaaccgaagg caccattaag gctgatgaag
    16861 caagcgtcgc cagcgcgcag ctgcaactcg actggagccg tattaccgca ccagtcgatg
    16921 gtcgcgttgg tctcaagcag gttgatgttg gtaaccaaat ctccagtggt gataccaccg
    16981 ggatcgtggt gatcacccag acgcatccta tcgatttgct ctttaccctg ccggaaagcg
    17041 atatcgctac cgtcgtgcag gcgcaaaaag ccggaaaacc gctggtggta gaagcctggg
    17101 atcgcaccaa ctcgaagaaa ttaagtgaag gcacgctgtt aagtctcgat aaccaaatcg
    17161 atgccactac cggtacgatt aaagtgaaag cacgctttaa taatcaggat gatgcgctgt
    17221 ttcccaatca gtttgttaac gcgcgcatgt tagtcgacac cgaacaaaac gccgtagtga
    17281 tcccaacagc cgccctgcaa atgggcaatg aaggccattt tgtctgggtg ctgaatagcg
    17341 aaaacaaggt cagcaaacat ctggtgacgc cgggcattca ggacagtcag aaagtggtga
    17401 tccgcgcagg tatttctgcg ggcgatcgcg tggtgacaga cggcattgat cgcctgaccg
    17461 aaggggcgaa agtggaagtg gtggaagccc agagcgccac cactccggaa gagaaagcca
    17521 ccagccgcga atacgcgaaa aaaggagctc gctcctgatg caagtgttac ccccgagcag
    17581 cacaggcggc ccgtcgcgcc tgtttattat gcgtcctgtg gccaccacgc tgctgatggt
    17641 ggcgatctta ctcgccggga ttatcggtta tcgcgccctg cccgtttcgg cgctgccgga
    17701 agtggactat ccgaccattc aggtggtcac gctctactca ggtgcgagcc cggatgtcat
    17761 gacctctgcc gttaccgcgc cgctcgaacg ccagttcggg cagatgtctg gcctgaaaca
    17821 gatgtcgtcg caaagttccg gcggtgcgtc agttatcact ttacagttcc agctaacatt
    17881 accgctggat gtcgccgagc aggaagtgca ggccgcgatt aacgctgcga ccaacttgtt
    17941 gccgagcgat ctgcctaacc cgccggttta cagcaaagtg aacccggcag atccgccgat
    18001 catgacgctc gccgtcacct caacagccat gccgatgacg caagtggaag atatggtgga
    18061 aacccgcgtc gcgcagaaaa tctcgcagat ttccggcgtc ggcctggtga cgctttccgg
    18121 cggccagcgt ccggctgttc gcgtcaaact taacgctcag gcgattgccg ccctcggcct
    18181 gaccagcgaa accgtgcgca ccgccattac cggcgctaac gttaactcgg ctaaaggtag
    18241 ccttgacggc ccttcccgtg cggttacgct ttccgctaac gaccagatgc aatccgccga
    18301 agagtatcgc cagctaatca tcgcctacca gaacggcgcg ccgattcgtc tgggcgatgt
    18361 cgcaactgta gagcaaggtg cagaaaacag ctggctcggc gcatgggcga ataaagaaca
    18421 ggccattgtg atgaatgttc agcgccagcc cggtgctaac attatctcca ccgccgacag
    18481 catccggcag atgctgccac agctcaccga gagtctgccg aaatcggtga aggtgacggt
    18541 actttctgac cgtaccacca atatccgcgc atccgtcgat gatactcagt ttgaattgat
    18601 gatggctatc gcgctggtag tcatgattat ctacctgttt ttgcgcaata ttccggcgac
    18661 catcattccc ggcgttgcag tgccgctgtc gttaatcggc accttcgcgg tcatggtgtt
    18721 tctcgatttt tcaatcaata acctgacgct gatggcgtta actatcgcca ccgggttcgt
    18781 ggtcgatgac gccatcgtgg tgatcgaaaa tatttcccgc tatatcgaaa aaggcgaaaa
    18841 accgttggcg gcggcgctca aaggcgcagg tgaaatcggc tttaccatta tctcgctgac
    18901 cttctcgctg attgcggtgt tgatcccact gctgtttatg ggcgatatcg tcgggcgact
    18961 gttccgcgaa tttgctatta ccctggcggt agcgatttta atctcagccg tggtgtcgct
    19021 aaccctgaca ccgatgatgt gcgcacggat gctcagccag gagtcgttgc gtaaacagaa
    19081 ccgcttctcc cgtgcctcgg aaaaaatgtt cgacaggata atcgccgcct atggtcgtgg
    19141 actggcgaaa gtgctgaatc atccgtggct gaccttaagc gtggcgctca gcacgctgct
    19201 gcttagcgtt ctattgtggg tgttcattcc gaaaggtttc ttcccggtac aggataacgg
    19261 cattattcag ggcactttgc aggcaccgca atccagctcc tttgccaata tggcccagcg
    19321 gcaacgccag gtcgcggacg tgattttgca ggatccggca gtgcaaagtc tgacctcatt
    19381 tgttggcgtt gatggcacta acccgtcgct gaacagtgcg cgtttgcaga tcaacctcaa
    19441 accgttggat gaacgtgatg atcgggtgca aaaagtcatc gcccgtctgc aaacggcggt
    19501 ggataaagtg ccgggcgtcg atctcttcct gcaaccaacg caggacctga ccatcgatac
    19561 tcaggtcagc cgcacccagt accagtttac cttgcaggcc acgtcactgg atgcgctcag
    19621 tacctgggtg ccacagttga tggaaaaact ccagcagctg ccacagattt ctgatgtctc
    19681 cagcgactgg caggacaaag ggctggtggc gtatgtcaat gttgatcgcg acagcgccag
    19741 ccgtctgggg atcagcatgg cggatgtcga taacgccctg tacaacgcgt ttggtcagcg
    19801 actgatttcc actatttata ctcaggctaa ccagtatcgt gtagtgctgg agcacaatac
    19861 cgaaaatacc ccaggcctcg cggcgctgga taccattcgc ctgaccagca gcgacggcgg
    19921 cgtggtgccg ctaagctcaa ttgccaaaat tgagcagcgt tttgcgccgc tttccatcaa
    19981 ccatctggat cagttcccgg taacgaccat ctcctttaac gtgccggata actattcgct
    20041 gggcgatgcg gtgcaggcaa ttatggacac cgagaagacg ctgaatctgc cggtggatat
    20101 caccacgcaa ttccagggca gcaccctcgc cttccagtcg gcgctgggca gcactgtctg
    20161 gctgattgtc gcggcggtgg tggcgatgta tatcgtgctc ggcattctgt acgagagctt
    20221 tattcacccg atcaccattc tctcgacgct acccacggca ggtgttggcg ccctgctggc
    20281 gttaatgatt gccggtagcg aactggatgt gattgcgatt atcggcatta ttttgctgat
    20341 cggtatcgtg aagaagaacg ccatcatgat gatcgacttc gcgctggctg ctgagcgcga
    20401 gcaaggcatg tcgccgcgcg atgcaatcta ccaggcttgt ctgttgcgtt ttcgtccgat
    20461 cctgatgacc accctggcgg ctctgcttgg cgcgctgccg ctgatgttga gtaccggggt
    20521 cggcgcggaa ctacgtcgtc cgttaggtat cggcatggtc ggcggcctga ttgtcagcca
    20581 ggtgctgacg ctgttcacca cgccggtgat ttatctgctg tttgaccgcc tggcattgtg
    20641 gaccaaaagt cgctttgctc gccatgaaga ggaggcgtaa gtgaagtttt ttgccctctt
    20701 catttaccgc ccggtggcga cgattttact gtcggtcgcc attaccctgt gcggcatact
    20761 gggtttccgt atgctgccgg tcgccccgct gccgcaggtc gattttccgg tgattatggt
    20821 cagcgcctcg ctgcccggtg cgtcgccgga gacaatggcg tcttccgttg ccacgccgct
    20881 ggagcgctca cttgggcgca ttgccggggt cagtgaaatg acctccagca gttcgctcgg
    20941 cagcacgcgt attattttgc agtttgattt tgaccgggat atcaacggtg cggcgcgtga
    21001 tgtgcaggcg gcgatcaacg ccgcacaaag tctgctgccc agtgggatgc ccagccgccc
    21061 gacttatcgc aaagcgaacc cgtcggatgc gccaattatg atcctcacgc taacctccga
    21121 tacttattcg cagggtgaac tgtacgattt cgcctcgacg cagctggctc cgacgatttc
    21181 gcaaatcgac ggtgtcggtg atgtcgatgt cggcggcagc tcgctgcccg ccgtgcgcgt
    21241 cgggctgaac ccgcaggcgc tgtttaatca gggcgtgtcg ctggacgacg tacgcactgc
    21301 catcagcaat gccaacgtgc gtaaaccgca gggggcgctg gaagatggca ctcaccgctg
    21361 gcagatccag accaacgatg aactgaaaac cgccgctgaa tatcagccgc tgattattca
    21421 ctacaacaac ggcggcgcgg ttcgtctggg cgatgtggcg acggtgaccg actcagtgca
    21481 ggatgtgcgc aacgccggga tgaccaacgc caaaccggct attttactga tgatccgcaa
    21541 attgccggaa gccaatatta tccagacggt agacagcatc cgggcaaaat taccggagtt
    21601 gcaggaaacc attccggcgg cgattgatct gcaaattgcc caggatcgct cccccaccat
    21661 tcgcgcctcg ctggaagaag tcgagcaaac gctgattatc tcggtggcgc tggtgattct
    21721 ggtggtcttt ttattcctgc gctcgggtcg cgccactatt attcccgccg ttgcggtgcc
    21781 ggtttcgctg attggtacgt ttgcggcgat gtacctgtgc ggattcagtc tcaataacct
    21841 ttcgttaatg gcgctcacca tcgctactgg tttcgtggtg gatgacgcca tcgtggtgct
    21901 ggaaaacatt gcacgtcatc tggaagcggg aatgaaaccg ttgcaagccg cactgcaagg
    21961 tactcgcgaa gtcggtttta cggtgctgtc gatgagtctg tcgctggtgg cggtgttcct
    22021 gccgctgctg ttgatgggcg gattgccggg ccgactgtta cgcgagtttg ccgtgacgct
    22081 ttctgtcgcc attggtattt cgttgctggt ttctctgaca ttaacgccaa tgatgtgtgg
    22141 ctggatgctg aaagccagca agccgcgcga gcaaaagcga ctgcgtggtt ttggtcgcat
    22201 gttggtagcc ctgcaacaag gctatggtaa gtcactgaaa tgggtgctca atcatacccg
    22261 tctggtgggc atggtgctgc ttggcaccat tgctttgaat atctggctat atatctcgat
    22321 tccgaaaacc ttcttcccgg agcaggacac tggcgtgttg atgggcggga tacaggcgga
    22381 tcagagtatt tcgtttcagg cgatgcgcgg taagttgcag gatttcatga aaattatccg
    22441 tgacgatccg gcagtggata atgtcaccgg ctttaccggc ggttcgcgag tgaacagcgg
    22501 gatgatgttt atcaccctca agccacgcga cgaacgcagc gaaacagcgc agcaaattat
    22561 tgaccgtcta cgcgtgaagc tggcgaaaga accgggggcg aatctgttcc tgatggcggt
    22621 acaggatatt cgcgttggcg gacgtcagtc gaacgccagc taccagtaca cgttgttatc
    22681 cgacgacctg gcggcactgc gcgaatggga gccgaaaatc cgcaaaaaac tggcgacatt
    22741 gccggaactg gcggacgtga actccgatca gcaggataac ggcgcggaga tgaatctggt
    22801 ttacgatcgc gacaccatgg cacggctggg aatcgacgta caggccgcca acagcctgtt
    22861 aaataacgcc ttcggtcagc ggcaaatctc gaccatttac cagccgatga accagtataa
    22921 agtggtgatg gaagtggatc cgcgctatac ccaggacatc agcgcgctgg aaaaaatgtt
    22981 cgtaatcaat aacgaaggaa aagcgatccc gctgtcgtat ttcgctaaat ggcaaccggc
    23041 gaatgcccca ctatcggtga atcatcaggg attatcggca gcctcgacca tctcgttcaa
    23101 cctgccgacc ggaaaatcgc tctcggacgc cagtgcggcg atcgatcgcg caatgaccca
    23161 gcttggtgtg ccttcgacgg tgcgcggcag ttttgccggc acggcgcagg tgttccagga
    23221 gacgatgaac tcacaggtga tcctgattat cgccgccatc gccacggtgt atatcgtgct
    23281 gggtatcctt tacgagagtt acgtacatcc gctgacgatt ctctccaccc tgccctcggc
    23341 gggcgttgga gcgctgctgg cgctggagct gttcaatgcc ccgttcagcc taatcgccct
    23401 gatagggatc atgctattaa tcggcattgt gaagaaaaac gccattatga tggtcgattt
    23461 tgcgcttgaa gcccaacggc acggtaacct gacgccgcag gaagctattt tccaggcctg
    23521 tctgctgcgt tttcgcccga ttatgatgac taccctggcg gcgctgtttg gcgcgctgcc
    23581 gctggtgttg tcgggcggtg acggctcgga gctgcggcaa cccttgggga tcaccattgt
    23641 cggcggtctg gtgatgagtc agctcctgac gctgtatacc acgccggtgg tatatctctt
    23701 tttcgaccgt ctgcggctgc gtttttcgcg taaatctaaa caaacggtaa ccgagtaaat
    23761 gacagatctt cccgacagca cccgctggca attgtggatt gtggctttcg gcttctttat
    23821 gcagtcgctg gacaccacca tcgtaaacac cgcccttccc tcaatggcgc aaagcctcgg
    23881 ggaaagtccg ttgcatatgc acatggtcat tgtctcttat gtgctgaccg tggcggtgat
    23941 gctgcccgcc agcggctggc tggcggacaa agtcggcgtg cgcaatattt tctttaccgc
    24001 tatcgtgctg tttactctcg gttcactgtt ttgcgcgctt tccggcacgc tgaacgaact
    24061 gttgctggca cgcgcgttac agggcgttgg cggtgcgatg atggtgccgg tcggcaggtt
    24121 gacggtgatg aaaatcgtac cgcgcgagca atatatggcg gcgatgacct ttgtcacgtt
    24181 acccggtcag gtcggtccgc tgctcggtcc ggcgctcggc ggtctgctgg tggagtacgc
    24241 gtcgtggcac tggatctttt tgatcaacat tccggtgggg attatcggtg cgatcgccac
    24301 attgatgtta atgccgaact acaccatgca gacgcggcgc tttgatcttt ccggcttttt
    24361 attgctggcg gttggcatgg cggtattaac cctggcgctg gacggcagta aaggtacagg
    24421 tttatcgccg ctggcgatta ctggcctggt cgcagttggt gtggtggcac tggtgcttta
    24481 tctgctgcac gccagaaata acaaccgcgc tcttttcagt ctgaaactgt tccgcactcg
    24541 taccttttcg ctgggcctgg cggggagctt tgccggacgt atcggtagcg gcatgttgcc
    24601 ctttatgacg ccggttttcc tgcaaattgg ccttggcttc tcaccgtttc atgccggact
    24661 gatgatgatc ccgatggtgc ttggcagcat ggggatgaag cggattgttg tacaggtggt
    24721 gaatcgcttt ggttatcgtc gggtactggt agcgaccacg ctgggtctgt cgctggtcac
    24781 cctgttgttt atgactaccg ctctgttggg ctggtactac gttttgccgt tcgtcctgtt
    24841 tttacaaggg atggtcaact cgacccgttt ctcctccatg aacaccctga cgctgaaaga
    24901 tctcccggac aatctggcga gcagcggcaa cagtctgctg tcgatgatta tgcaattgtc
    24961 gatgagtatc ggcgtcacca tcgccgggct gttgctggga ctttttggtt cgcagcatgt
    25021 cagcgtcgac agcagcacca cacaaaccgt ctttatgtac acctggctta gcatggcgtt
    25081 tatcatcgcc cttccggcgt tcatctttgc cagagtgccg aacgatacgc atcaaaatgt
    25141 agctatttcg cggcgaaaaa ggagcgcgca atgaagttct ggcgacctgg tattaccggc
    25201 aaactgtttc tggcgatttt cgccacctgc attgtcttgc tgatcagtat gcactgggcg
    25261 gtgcgtatca gttttgagcg tggctttatt gattacatca agcatggtaa tgaacagcga
    25321 ttacaactgt taagtgatgc gcttggcgag cagtatgcgc agcatggcaa ctggcgcttc
    25381 ctgcgcaaca atgatcgctt tgtctttcag atcctgcgtt catttgaaca cgataattcg
    25441 gaagataaac ccggcccggg tatgccacca cacggctggc gtactcagtt ctgggtggtt
    25501 gatcaaaaca acaaggtgct ggttggtccg cgagcgccga ttccacctga cggtacacgg
    25561 cgacccattc tggtcaacgg tgcggaagtg ggcgcggtga ttgcctcccc tgttgagcgg
    25621 ctgacccgca atactgatat caatttcgat aaacaacagc ggcaaaccag ctggctgatt
    25681 gtcgccctgg caacgttact cgcggcactc gctacttttc tgctggcgcg cggtttgctg
    25741 gcaccggtaa aacgacttgt cgatggcacg cacaaactgg cggcgggcga tttcactacc
    25801 cgcgtaacgc ccaccagtga agatgaactg ggcaaactgg cgcaagactt caaccagctc
    25861 gccagcacgc tggagaaaaa ccagcaaatg cggcgcgatt ttatggccga tatttctcac
    25921 gaactgcgta cgccattagc ggtgctgcgc ggtgaactgg aagccattca ggatggcgtg
    25981 cgcaaattca cgccggagac ggtggcgtct ttacaggcgg aggtcggtac actgaccaaa
    26041 ctggttgacg atcttcatca gttgtcgatg tctgatgaag gcgctctcgc ctatcaaaaa
    26101 gcaccggtag atttgatccc actgctggaa gtggcaggcg gcgcatttcg cgaacgattt
    26161 gccagccgtg gcctgaaact gcaattttcc ctgccagaca gtatcaccgt atttggcgat
    26221 cgcgaccgtt taatgcagtt attcaataac ttactggaaa acagcctgcg ctacactgac
    26281 agcggcggca gcctgaaaat ctctgccgag cagcacgaca aaacggtgcg cctgaccttt
    26341 gccgacagtg cgccaggtgt cagtgacgat cagctacaaa aattgtttga acgtttttat
    26401 cgcaccgaag gttcccgcaa ccgcgccagc ggcggttccg ggctggggct ggcgatttgc
    26461 ctgaacattg ttgaagcaca taatggccgc attattgccg cccattcgcc ttttggcggg
    26521 gtaagcatta cagtagagtt accgctggaa cgggatttac agagagaagt atgaccgagt
    26581 taccaatcga cgaaaacaca ccgcgtattt tgatcgtgga agatgaaccg aagctgggac
    26641 agttgctcat tgattatctg cgtgctgcga gctatgcgcc gacgcttatc agccacggcg
    26701 atcaggtact gtcgtacgtg cgccagacgc cgccggatct gatcctgttg gatctgatgc
    26761 tccctggcac cgatggcctg acgctgtgcc gggaaattcg tcgtttttct gacattccta
    26821 tcgtgatggt gacagcaaaa atcgaagaga tcgaccgcct gctagggctg gagattggcg
    26881 cagatgacta catttgcaag ccgtacagcc cacgggaagt ggtagcgcgt gtcaaaacca
    26941 ttttgcgccg ttgcaaaccg cagcgtgagt tgcagcaaca ggatgctgaa agcccgttga
    27001 ttatcgacga aggtcgtttt caggcttcat ggcgcggtaa aatgcttgac ctgacgcctg
    27061 cggaatttcg tctgctgaaa acgctctctc atgaaccagg aaaagtgttc tcccgcgagc
    27121 aattgctcaa tcatctttat gacgactacc gcgtagtaac cgaccgtacc atcgacagcc
    27181 acattaaaaa cctgcgccgc aagctggaat ctctcgacgc cgaacagtca tttatccgcg
    27241 ccgtttacgg cgtcggttac cgctgggaag ccgacgcctg ccgcatcgtt tagttttagc
    27301 gacattactt tgttagccgg agatggcatt cagaccagat ccggcaacat tatcccacgc
    27361 atggtcagca aactgacatt atctctccgc cctggcaggc atcagaaaat cgctcatact
    27421 ttaatcggta aacagcacct ttagatgctg ttttcgatac gcaatttcaa tcaaggagtc
    27481 attatggctg gttggtttga actcagtaag agcagtgata gtcagttccg gtttgtgcta
    27541 aaagcgggca atggtgagac gatcctcacc agcgagctgt atacctcaaa agcctctgcg
    27601 gaaaagggca tcgcgtcggt gcgtagcaac agcccgcaag aagaacgcta tgagaaaaaa
    27661 acggcaagca acggcaaatt ctatttcaat ctgaaagccg ccaatcatca aattatcggc
    27721 tccagccaga tgtatgccac cgcgcaatct cgtgaaaccg gaattgcctc cgttaaagcc
    27781 aatggcacaa gccagacggt gaaagacaat acgtaattac gatgccgggc gtgggttgtt
    27841 ggcgtccggc gctttaccag atccccccta cagcgctaca atgcccgccc ttaaagtggg
    27901 ggcactcccc taaccgcttc atcaggtgaa gcggatctga cctgtcatca gaacgagaga
    27961 attatgttta aaccggaact cctttccccg gcgggaacgc tgaaaaatat gcgttacgct
    28021 ttcgcttatg gcgcagatgc tgtttatgcg ggccagccgc gttactccct gcgtgtgcgc
    28081 aacaacgaat tcaaccacga aaaccttcag ctcggcatca atgaagccca cgcgctgggg
    28141 aaaaagtttt atgtcgtggt caacattgca ccgcacaacg ccaagctgaa aacctttatc
    28201 cgtgacctga aaccggtggt ggaaatgggg ccggatgcgc tgattatgtc cgatccaggg
    28261 ctgattatgc tggtgcgtga gcacttccct gaaatgccga tccatctctc ggtacaggct
    28321 aacgccgtga actgggcgac ggtgaaattc tggcagcaaa tgggactgac ccgcgtgatc
    28381 ctctctcgcg agctgtcgct ggaagagatt gaagagatcc gcaatcaggt gccggatatg
    28441 gagatcgaga tcttcgttca cggcgcactg tgcatggcct actccggtcg ctgcctgctc
    28501 tctggctata tcaacaagcg cgatccgaac cagggtacct gcaccaacgc ctgccgctgg
    28561 gagtacaacg tccaggaagg gaaagaagat aacgttggca acatcgtaca caagtacgag
    28621 ccgattccgg tgcaaaatgt tgagccgacg ctgggtatcg gcgcgccaac cgacaaagtg
    28681 tttatgatcg aagaagccca gcgtccgggc gagtatatga ccgcgtttga agatgagcac
    28741 ggcacttaca tcatgaactc gaaagatctg cgcgccatcg cccatgtaga acgcctgacc
    28801 aaaatgggcg tgcattcgct gaaaatcgaa ggccgtacta aatctttcta ctattgcgca
    28861 cgcaccgcac aggtttatcg taaagctatc gatgatgccg ctgcgggcaa accgttcgat
    28921 accagcctgc tggaaactct ggaaggtctg gcgcatcgtg gctataccga aggtttcctg
    28981 cgtcgtcata ctcacgacga ttatcagaac tacgaatacg gttattcagt ttctgaccgc
    29041 cagcagtttg ttggtgagtt taccggtgag cgcaaggggg acctcgcggc ggtagcggtg
    29101 aaaaataaat tctccgttgg cgacagcctt gagctgatga cgccgcaagg caacattaac
    29161 tttacccttg agcacatgga aaacgccaaa ggcgaagcta tgccggtagc accaggcgat
    29221 ggttatactg tgtggctccc ggtgccgcag gatcttgagc taaattacgc gctgctgatg
    29281 cgtaatttct ccggggaaac tacgcgtaac ccccacggta agtgattaat ttcgattatt
    29341 tttcccggat ggaaaattct tagaaaccga tcacatacag ctgcatttat taaggttatc
    29401 atccgtttcg ctgaaaaaca taacccataa aatgctagct gtaccaggaa ccacctcctt
    29461 agcctgtgta atctccctta cacgggctta ttttttacgc gtaatacaat gaaataaaag
    29521 gatttatttc tggtcacgtc cacatattga ccacatcgac aaaaaaagcc cctcaactga
    29581 ggggctttct gtttgtaatt acatccacat aatttgctgc cctgacggca acgggtgcgg
    29641 tcttacggcg tggacttctc ccggcttcac gatgtatcgc tgtaccgact cataagtgat
    29701 gaacgtggcg ctgcaattca cgttctgaca ctggtgataa cgctcttttg tcgtgtcagt
    29761 gatatagcga cttgtacgcg catgtgcggc atgctggcat aaaggacaat gaaacatcgc
    29821 gagcacctct tccggttttg ttgatggtgc cattttagtt aatttatcct tataaaacaa
    29881 acagataaaa taaaaacatc attcatcatc ttctgtttcg tattccacat cagaaagcct
    29941 gacctcaagc tctaaggacg tcgtgaagcc gctattattc agaaaatgtg tcaccttagt
    30001 gatggtccag tcctgctcgt ctatgacgcg cttaaagcct gacactctga ccggtgtttc
    30061 cgtgtaaata tctgcacgac cggtagccag gatgatggag aactccgcaa cgccccgttg
    30121 cagtttgtcc cacttcgcct gagcagcacg catggcctgt gctttcgtgg catataccgt
    30181 ggtcagggca aaaacgttgt cagcctcacc ggccatgtat tcaccttcgc gcgcttccgg
    30241 tacttttggc gctttcttct gcctgaccgg tttcgctttc gggtgctcca gtgcgcgcag
    30301 gtgtttctct ttctttttgc gtttcagttt taccttctgc ttttgcggtt tcgggtcttt
    30361 ggtgtgtaac cactttgccg ttacaccggt ataggctcca cggtcagcaa tcgcaaaatg
    30421 atggcggtcg ccgtcgctgc gggttatggt gacctgcggg atttttttac cgctggccgt
    30481 caccccctgc cccgctttga gaaacaacag ttttcccatt tttaccgaga cctcaccgcc
    30541 gttgcgttca gcaaggcggg tcaggaattt tgcatcagac tcctgcgact ggtcgatgtg
    30601 cgggatttta attccggcca gtgacggcgc gacactggct tccagcctgt tacgggaggc
    30661 tattgcctca acaatcgcac cgagcgtggt gtcatgccag gagtcttcac ggcgggaatt
    30721 gagcgtcccg cgaaaatctg cactccgggc gcggatggtg accacatccg gcgcgccccg
    30781 gtgttcaacc tcatcaacgg taaatttccc tttgcatacc agggcaaaac ctttccagcc
    30841 gatatacacc gtcaggacag cgccacgaat cggcagcccg acctgcccgt cggcatcgtt
    30901 cagttcaata tcaagctggt cagcctcaaa gccccggtta tccgtcagag tcatgctcat
    30961 cagacggtcg ctgatattgc cggtaatatc cctgctgtcg agcatcagca tgtaatccgg
    31021 cgtcagcgta ctgcctgcat caaatgtcag tgcatccagc attatcccgc ccccgtcata
    31081 cctgtgaatt tagtcgccat actgccagcc ttaccgatga gcgattccgc ctgtttaccg
    31141 atatcgccat aaagcgcggc cagtggttcg tcaacgcggg tgagcgacag cgtaaaatca
    31201 atttttcggg gtgtgccgtc tgcaaagaaa atactccctg tttcactcac cctgctgatg
    31261 acatacatgc cgtaaatcat gccggtgcca tccagcaacg gccacgcccg accttcctct
    31321 gccatcagcc tgagcgtggt catcgtcagc ttgccgccgg tcagttcggg ataaagcaca
    31381 ccggccagcg tcatgttttc ctcgccaacg ccgagaaact ggtaggcatc ccgtttaccg
    31441 atacgggaat ttgacggcca gcgataatct gattcacgct gcatggtctg gtgtggcagc
    31501 gtctggcgca taaaaacaaa catacctaac gcgagcatca tttttcgtca cctccttaac
    31561 cgtcatgcat catgctggca cgggcgcgcg cacgtttatc ccgctcgtat ttttcgagcg
    31621 catcctgtaa ctggcggtca agctgtgtcc ccggcgcagt accacccgtc aggctgatgt
    31681 gatattcgtt tttactctgg tccacataag agcggccagc cggtgccgtg accggctgat
    31741 aagcctgata gcctgcataa gagctggtcg ccggaatata accaccggtg ccatacgtgg
    31801 cggcatgagt tctggcggcg gtctggtcaa gtgtgtctga ctctttgttg ataacaccga
    31861 gtttttccag tacccagtca ataccactgc gcagtttgtt gaacgcatta agcggcagca
    31921 tcaacgcgtc agccagtgcc tgcccgaaca tgacgcccgt gtcacggcaa cggttcaggg
    31981 tgtcctgggt ggctttgacc ggggcaatca gttttttaaa ccactgccac gcggcctgta
    32041 acttttcacc cagccagtca aacagcggct taagtggcgt gaacagttcc cccaccggcg
    32101 caaatgcagc tttcagccct tcaaccacac cgccaaagaa tgcgctgaca ggctcccagt
    32161 atttacggat aagcaacgcc ccggcgacaa ttgcagccac cacggccaca accggccagc
    32221 taatcgcccc gatggccgtc ataacagcac tgccaaccgt cgtgaagatt gcccccattg
    32281 cgcctgctgc cgcgatgatg gcattaatgc cggtgataac cggccaggct acaagaccaa
    32341 ttgcaccgat gatgccaata agcgccagtg cgccaccgac aataaggccg atggttgacg
    32401 ccagtgattt gtttttctgg atccagccgt cgagttttaa cacatacttt gtggccgtct
    32461 gcgtgagatt acgcagtgcg ccttcctgtt ggtcaaacag gtcagtcccc accgcctcat
    32521 aagcggactg aaactcctta aagtcaccgc cgaggttgtc ctgcatgata tttaccagct
    32581 ctgcggtctt cccgtctgag gctttaaacg cagcggtcag tttgtccagc tttccggttg
    32641 aggcggcagt catcagcacg gcggcggctg aactggcctc ctccccgaaa atggttttca
    32701 tgtattcagc ctgctgggca gtaccgagcc ggtttttctc aaaactggcc tgcatttctt
    32761 tcagaatggt aaatattggt cgggtattcc ccttgctgtc tgaggttttc actccaagct
    32821 ctttgagtgc atcccatgct tttcccgtcg gtgcctgcag gcgacttaac acggcacggc
    32881 ttcccgtccc cgccattgag cctgtgattt ttgcatcatg cagcgccccg accattgcgg
    32941 cggtttcttc aatgctgaca ccggcatttt ttgccacagg tgcggcatag gtcagcgcat
    33001 cgctcatacc gtcaaaatcg gcggcggttt tgttcatcgt catggagaga acatccccga
    33061 tatgagcaac cttatcgttt gaaagctgaa aggcagattt catccccatc agcagggcgg
    33121 cgttttcttc catcgtgcga cggttcgcca gcgccatatt cagcgtgacc ggcgttgccg
    33181 cctgaatggc atcaacatcc ccacccgctt tcgcgatgat aatctgtgca ccggccgcat
    33241 catccgccga ggcggcggta ttgtcgccga gctggcgcgc ctgcttgcgt agcgcggcca
    33301 tttcggcgga gtcttttgcc acaccgagca cggcctgtaa ttctgagttt ttctgcgcaa
    33361 attcataacc gggcatcagc agcttaacac cggccatcgt tcccgccgcc gcaatcccca
    33421 caccggcagc gcccactgag gccatatttc cggccagctc ctttcctgcc tgatagcgct
    33481 gttttactgc gttaagtttt gcctgttgcg cactgacacg cgccagcgcg tcacgctgcc
    33541 ggttaagctg tgcggtggtt tcactgatac ggtttttcag ccccagctca tcatgtgcaa
    33601 gattgcgggt attaattccc acagcggcca gttcccgctg ctggcgttta acggaatccg
    33661 tcaggcggtt atatttcgcc tgtaagtcct ccgccgcacg ctttgcggat tccagcactt
    33721 tcgcctgagc acgggtcgga cgttcggtgt ttttaaactg tgtggcaagg gcttcggcct
    33781 cctgccgtgc cttttcaagt gcatgaccag tcacggcgag ctgtgcgctg gtcttgcgga
    33841 atccctcaat acgggatgcc tgcccgttca gctcgcgcag tgatttttgt gtttcccgga
    33901 tatcccccga cagcgactta ctcgctgtgc ggatggattt aaacgggcgg gatgcctggt
    33961 caacagccct gagcaatacc tgtaatttta cattgttact cattcgtgtt tccgcttcgc
    34021 ctgagcgcct tttcgcgcca tgtgatgagt tcggtcaggc tcatgggata cagttctgat
    34081 ggcggccagt gaaataccac tgccacatcc gccatcaggt catcgaccga gagatttttc
    34141 ggaaacgtca ctgcaccgag ttcggcgaca aaaaaccgac caccttaccg gccagcgcca
    34201 caaggtcagg cagttccagc gcggcgacct cctgttcggt cagcatcggt gccgtcatgc
    34261 gcggcagcac tttaatcagt gcatcgactt cggagttcgc gaccgcagcc agactgacac
    34321 cgcgcagcgt cccggcattg ggtttcatca gcgtgacctg ttcgataacc tgctcaccac
    34381 gcttgaccgg attgtccagg gtaatcacat tttctttgtt catggttttc tcacttctga
    34441 atcagggtta accggtcagc ctggctgacc ggatgaaaat cacaggccga tattgcggcg
    34501 gtgttgctcc agccggtcga cgccgttcac cttctcaatc atgttgatgg tgtcgatttc
    34561 gaccagctcc ttaccgtcca tcgtcagccg gaaataggtg cagaccacgg agattttcga
    34621 ctcggtgtct tctccctgtt taccctcgcc ggtgtcgatt tctttctgac ggccacgcat
    34681 gaccacctcg acggccaccg tttcgccggt atcgtcgcgc tggtaagagc ctgcaaaacg
    34741 aatcggtacg gcatccacac cggttgcggc gtaaagctcc cagataaccg aatccgggaa
    34801 gccaccgagc gaccactcca ttgacagcgc atcgtcatca aggccgaggt ctaccggtgc
    34861 gctgccattc atccccgcac cgcgatagtt ttcgagctta cgggtcagtt ttggcagcgt
    34921 gacggacttc gcgacgccct gatagctgta gccgttcaga aagacgttca ttaacttgag
    34981 tttgcgcggc attgccatcg gtcaggctcc ttaattgctg ttaaccgagg tgaccagatt
    35041 tgccaggtat ttatcggtga tacgctggcg cagggtcagg ttttcaagag gaggtaccgg
    35101 tgtatagtcg tagtcgatat acagttttcc ggccttgagg gtttccgcat cgctggattc
    35161 ttcgctgaac cagcaggtcg catccacgat atagccgttt gttttcagct cacggaattt
    35221 ggcattgatg ccgtcaacga tgtcgcgaat cagcgttgcg gtgatgggct tgtccaccgc
    35281 ccacatgtgc gcctcagcca tcgtgtcggc cagcacctgc gcggtgcggg tgtagttttc
    35341 aaagaggaac agcgggtcat cagtgcaggt acggttaccc cagaagcgga aaccgtcgcg
    35401 gcgaatcagc gtagtgacgc ctgactcgtt aagcaggtca gcatcggtgc cggactcctg
    35461 caaatcccag aatacagatg cgctgatgcc gttaacaccg tttaccccga cgttggacag
    35521 cgttttatgc cagccctgct cctggtcgat tttagcgcgc agacccagcg cacgggcggt
    35581 ggcatacgcg gtggcggtgg tgctgctgac cgtatcccat gcgaggaaat ccggccagat
    35641 gaccatcagc tcacgctggc tgaaattctg gcggtaggct ttcacctcgg aaatggtttt
    35701 acagccccat gcgctgatat acccgaaagc gcgcagcttc tgacagactg atgccagtgc
    35761 aacagccacc tctttggtgt ccagtcccgg cacgccgaga atacgcggtt taacaccggt
    35821 taccgactct gccgccagca gggctttcag tccggtatac tgaccgtttt cgtcagtggt
    35881 gccgatgata ttggaaacgg tctgcgcgag tttcgtttct tcgtcgtcgc cggtgccgtc
    35941 ttcaacacgc acgacaacgg taaccggttt tgactggtca gcgatggcct gcaacgatgc
    36001 cgccagcgtg ccttttttac cggcctttgc aattgcgctc tgcacattgg taatcagcac
    36061 aggtttattg agggggaagg tttccgcatc cgcatcgctg gccgtgcaga ccatgccgac
    36121 aatggcggtg gatacggtgg aaatgacgcg ggtgccgtcg ttaatctcca gcacctgcac
    36181 gccgtgatga taatcactca tccgtttaac tccgtggtta atgggtgagt gatattttca
    36241 gttgtgccgg agatgtcagg ctatttgtcc cggttggcta agggatgaca caatttattc
    36301 tttgtcgctg atgaggggaa ttttttatag agcgtggaca ggccaatatc aaaaatcagc
    36361 gccacacgtt gacgagattc ccccgcagcc agcaaccgtc cgacctgctc ccactcactc
    36421 gtggtgagtt tcggtcgtct gccaccaata cgacctttgg ctctggccgc ttccagtccg
    36481 gcgcgtgtcc gctcgacaat gagttctcgc tccatttctg ccagggcccc catcacatga
    36541 aagaaaaagc gccccatcgg cgtgctggta tcaatagcat ccgtcaggct gcgaaaatta
    36601 acgccgcgtt cgcgcagttc ctcaaccaga atgaccagat gccgcatact acgccccagc
    36661 ctgtccagct tccagactac cagagtgtca cctgctgata atgttctgag cagttttttc
    36721 agtcccggtc ggtcggactt cgtgccgctg attttatcct caaaaatcag ctcacatcct
    36781 gcacactcca gcgcattacg ctgcaattcc gtgttctggt catttgttga cacgcgtaca
    36841 tagccaataa gcatgagcat ctccctgaat aaaaaccgga gatgatgcca gttacccgtt
    36901 atttctgcat tttcataaac gttggtttgg gagaaggttc ggcattaccc gttggtgtgc
    36961 ctgttccgtg gccttcagcc actccgccga caggctggct gaaatgcaac ggggcggctt
    37021 tttctgctga agaatacccg gaactggcaa aggtttaccc gacaaataaa ttgcctgatt
    37081 tacgtggtga gtttattcgt ggctgggatg acggacgtgg agtggataac ggaaggggat
    37141 tattgacgct tcaggacggt gcgattgtca gtcataacca ctattgggga atctggactt
    37201 cacgaactaa cgaccagact ctggaaagtt ttacaggcac cacgatttta aaacaaatca
    37261 cgcccctgtc tccggccatt aacttcgata attacccaat tcccaacccg gctattacag
    37321 agggtggtgt tgttgcggca acgactaaac ctgcaggtgc gaatgaaaca cgcccacgaa
    37381 atgtcgcttt taactatatt gtgagggctg cataatgaat aacgcagaat taaacagtga
    37441 attaattgcc actatggcag gagaaattac tgtttataac tttgatgtca tgagtcggga
    37501 atatatttca gcttcaactg aatatcttgc tattggtgtc ggcattccgg catattcctg
    37561 tttagatgcc ccaggcgcat acaaagctgg ttatgcaatc tgccgttctg cagattttaa
    37621 ctcatgggaa tatgtgccag accatcgcgg tgaaatcgtc tttagcaccg aaacaggaga
    37681 atcaaaagaa atcaccgctc cgggtgatta ccctgataat acaaccacta tcgccccgtt
    37741 aacgccatac gataaatggg atggtgagaa atgggtgacg gataccgaag cacagcatag
    37801 cgccgcagta gaagcggcag aaacacaacg ccagtcgctg attgatactg caatggcttc
    37861 cattagtctg attcagctga aattacaggc cggacggaag ctgacgcagg cagaaaccaa
    37921 ccgacttaac acggtgctgg attacattga cgcggtgacg gcaacagata ccagcaccgc
    37981 gccggatgtc atctggcctg aactgccgga ggcgtaggcc attcaatatc tggcgcactg
    38041 gcggtatcga ccagctccag tgcgtccaga taatccagcc acaaattata ttgtgccagt
    38101 tcctcacctt tcagacgacc aatagcggct ttaccgggcc attgcttact gttcatgtat
    38161 tcgttggcct ggttaattag tagctgtctt tctgattcag taatttcaat aag
//
LOCUS       DAZKIP010000031        38053 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZKIP010000031 DAZKIP010000000
VERSION     DAZKIP010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.031"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            144..755
                     /gene="ruvA"
                     /locus_tag="ACHOUK_001851"
     CDS             144..755
                     /gene="ruvA"
                     /locus_tag="ACHOUK_001851"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFP9897314.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            764..1774
                     /gene="ruvB"
                     /locus_tag="ACHOUK_001852"
     CDS             764..1774
                     /gene="ruvB"
                     /locus_tag="ACHOUK_001852"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFP9897315.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(2020..2805)
                     /gene="znuB"
                     /locus_tag="ACHOUK_001853"
     CDS             complement(2020..2805)
                     /gene="znuB"
                     /locus_tag="ACHOUK_001853"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFP9897316.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTDGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(2802..3557)
                     /gene="znuC"
                     /locus_tag="ACHOUK_001854"
     CDS             complement(2802..3557)
                     /gene="znuC"
                     /locus_tag="ACHOUK_001854"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFP9897317.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3636..4568
                     /gene="znuA"
                     /locus_tag="ACHOUK_001855"
     CDS             3636..4568
                     /gene="znuA"
                     /locus_tag="ACHOUK_001855"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFP9897318.1"
                     /translation="MLHKKTLLFAALFAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSINGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4584..5906
                     /gene="mepM"
                     /locus_tag="ACHOUK_001856"
     CDS             4584..5906
                     /gene="mepM"
                     /locus_tag="ACHOUK_001856"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFP9897319.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            6026..6997
                     /gene="lpxM"
                     /locus_tag="ACHOUK_001857"
                     /gene_synonym="msbB"
     CDS             6026..6997
                     /gene="lpxM"
                     /locus_tag="ACHOUK_001857"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310592.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFP9897320.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRIAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(7128..8570)
                     /gene="pyk"
                     /locus_tag="ACHOUK_001858"
     CDS             complement(7128..8570)
                     /gene="pyk"
                     /locus_tag="ACHOUK_001858"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFP9897321.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGINYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(8698..9567)
                     /gene="hexR"
                     /locus_tag="ACHOUK_001859"
                     /gene_synonym="yebK"
     CDS             complement(8698..9567)
                     /gene="hexR"
                     /locus_tag="ACHOUK_001859"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFP9897322.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            9905..11380
                     /gene="zwf"
                     /locus_tag="ACHOUK_001860"
     CDS             9905..11380
                     /gene="zwf"
                     /locus_tag="ACHOUK_001860"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFP9897323.1"
                     /translation="MAVTQTAQVCDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            11615..13426
                     /gene="edd"
                     /locus_tag="ACHOUK_001861"
     CDS             11615..13426
                     /gene="edd"
                     /locus_tag="ACHOUK_001861"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFP9897324.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            13463..14104
                     /gene="kdgA"
                     /locus_tag="ACHOUK_001862"
                     /gene_synonym="eda"
     CDS             13463..14104
                     /gene="kdgA"
                     /locus_tag="ACHOUK_001862"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFP9897325.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(14160..15338)
                     /gene="purT"
                     /locus_tag="ACHOUK_001863"
     CDS             complement(14160..15338)
                     /gene="purT"
                     /locus_tag="ACHOUK_001863"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFP9897326.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYCESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            15472..15762
                     /gene="yebG"
                     /locus_tag="ACHOUK_001864"
     CDS             15472..15762
                     /gene="yebG"
                     /locus_tag="ACHOUK_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFP9897327.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            15829..16185
                     /gene="yebF"
                     /locus_tag="ACHOUK_001865"
     CDS             15829..16185
                     /gene="yebF"
                     /locus_tag="ACHOUK_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFP9897328.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            16512..17171
                     /gene="yebE"
                     /locus_tag="ACHOUK_001866"
     CDS             16512..17171
                     /gene="yebE"
                     /locus_tag="ACHOUK_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF533 domain-containing protein"
                     /protein_id="HFP9897329.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLIGGGAVAGTVLWNKYKDKIRAAHQDEPQFGSQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            17380..19440
                     /gene="ptrB"
                     /locus_tag="ACHOUK_001867"
     CDS             17380..19440
                     /gene="ptrB"
                     /locus_tag="ACHOUK_001867"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFP9897330.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRNHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDLQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     RPAD"
     gene            complement(19437..20099)
                     /gene="exoX"
                     /locus_tag="ACHOUK_001868"
     CDS             complement(19437..20099)
                     /gene="exoX"
                     /locus_tag="ACHOUK_001868"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFP9897331.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVHPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(20123..20779)
                     /gene="yobB"
                     /locus_tag="ACHOUK_001869"
     CDS             complement(20123..20779)
                     /gene="yobB"
                     /locus_tag="ACHOUK_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFP9897332.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEHGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            complement(20881..21111)
                     /gene="holE"
                     /locus_tag="ACHOUK_001870"
     CDS             complement(20881..21111)
                     /gene="holE"
                     /locus_tag="ACHOUK_001870"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFP9897333.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            21250..21624
                     /gene="yobA"
                     /locus_tag="ACHOUK_001871"
     CDS             21250..21624
                     /gene="yobA"
                     /locus_tag="ACHOUK_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFP9897334.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKRLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            21628..22500
                     /gene="copD"
                     /locus_tag="ACHOUK_001872"
     CDS             21628..22500
                     /gene="copD"
                     /locus_tag="ACHOUK_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFP9897335.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLTKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWKDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLLIISLLATLEPF"
     gene            22513..22854
                     /gene="yebY"
                     /locus_tag="ACHOUK_001873"
     CDS             22513..22854
                     /gene="yebY"
                     /locus_tag="ACHOUK_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFP9897336.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDVAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            23250..23906
                     /gene="pphA"
                     /locus_tag="ACHOUK_001874"
     CDS             23250..23906
                     /gene="pphA"
                     /locus_tag="ACHOUK_001874"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFP9897337.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLSERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTFVQLQ
                     "
     gene            complement(23907..24098)
                     /gene="yebW"
                     /locus_tag="ACHOUK_001875"
     CDS             complement(23907..24098)
                     /gene="yebW"
                     /locus_tag="ACHOUK_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFP9897338.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(24203..24439)
                     /gene="yebV"
                     /locus_tag="ACHOUK_001876"
     CDS             complement(24203..24439)
                     /gene="yebV"
                     /locus_tag="ACHOUK_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFP9897339.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(24557..25996)
                     /gene="rsmF"
                     /locus_tag="ACHOUK_001877"
     CDS             complement(24557..25996)
                     /gene="rsmF"
                     /locus_tag="ACHOUK_001877"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFP9897340.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKTSVADFLRLTAPYGWTLTPIPWCEEGFWIERDDEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAALPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRSGGTLVYSTCTLNREENESVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPTPKYKVGNFPFSPVKD
                     REAAQIRQAAASVGLNWDGNLRLWQRDKEVWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNENAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(26077..28710)
                     /gene="yebT"
                     /locus_tag="ACHOUK_001878"
     CDS             complement(26077..28710)
                     /gene="yebT"
                     /locus_tag="ACHOUK_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFP9897341.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTNNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEACSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(28679..29962)
                     /gene="yebS"
                     /locus_tag="ACHOUK_001879"
     CDS             complement(28679..29962)
                     /gene="yebS"
                     /locus_tag="ACHOUK_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFP9897342.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVTSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            30092..30589
                     /gene="msrC"
                     /locus_tag="ACHOUK_001880"
     CDS             30092..30589
                     /gene="msrC"
                     /locus_tag="ACHOUK_001880"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFP9897343.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            30686..31384
                     /gene="proQ"
                     /locus_tag="ACHOUK_001881"
     CDS             30686..31384
                     /gene="proQ"
                     /locus_tag="ACHOUK_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFP9897344.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTAKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            31404..33452
                     /gene="prc"
                     /locus_tag="ACHOUK_001882"
     CDS             31404..33452
                     /gene="prc"
                     /locus_tag="ACHOUK_001882"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFP9897345.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            33644..34525
                     /gene="htpX"
                     /locus_tag="ACHOUK_001883"
     CDS             33644..34525
                     /gene="htpX"
                     /locus_tag="ACHOUK_001883"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFP9897346.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(34571..35944)
                     /gene="yebQ"
                     /locus_tag="ACHOUK_001884"
     CDS             complement(34571..35944)
                     /gene="yebQ"
                     /locus_tag="ACHOUK_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9897347.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQILTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACISGLRI
                     TQPRSRA"
     gene            36121..36912
                     /gene="kdgR"
                     /locus_tag="ACHOUK_001885"
     CDS             36121..36912
                     /gene="kdgR"
                     /locus_tag="ACHOUK_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFP9897348.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(37056..37295)
                     /gene="yobH"
                     /locus_tag="ACHOUK_001886"
     CDS             complement(37056..37295)
                     /gene="yobH"
                     /locus_tag="ACHOUK_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFP9897349.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAELGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            37455..37598
                     /gene="mgrB"
                     /locus_tag="ACHOUK_001887"
     CDS             37455..37598
                     /gene="mgrB"
                     /locus_tag="ACHOUK_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFP9897350.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            37673..37960
                     /gene="yebO"
                     /locus_tag="ACHOUK_001888"
     CDS             37673..37960
                     /gene="yebO"
                     /locus_tag="ACHOUK_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFP9897351.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVEDEDIIRLVAER"
BASE COUNT         9744 a         9372 c         9816 g         9121 t
ORIGIN      
        1 tataacctgt agaataaatt atactgtgcc atttttcagt tcatcgagac acctcgcaag
       61 tttttttcat ccttcgctgg atatctatcc agcatttttt tatcatacag cattatcttt
      121 gattcattac gcaggagcgt catgtgatag gcagactcag aggcatcatc attgaaaaac
      181 aacccccgct ggtgttaatt gaagtgggcg gcgtaggcta tgaagtgcat atgccgatga
      241 cctgttttta tgaactccct gaagcgggtc aggaagcgat cgttttcacc cactttgtgg
      301 tgcgtgaaga cgcgcaactg ctgtacggtt ttaacaataa acaagagcgc acgttgttca
      361 aagagttgat caaaaccaac ggcgtcggcc cgaagttggc gctggcgatc ctctccggaa
      421 tgtcagcgca gcagttcgtt aatgccgttg agcgtgaaga agtgggggca ctggtgaaac
      481 tgccgggtat tggcaaaaaa accgccgaac gcttgattgt tgaaatgaaa gaccgattta
      541 aaggtttgca tggcgatctc tttacgccag ccgccgacct ggtactcacg tctcctgcca
      601 gcccggcgac cgacgatgct gaacaagaag cggttgccgc gctggtggcg ctgggctata
      661 aaccacaaga agcaagccgc atggtgagca aaatcgctcg ccctgacgcc agcagtgaaa
      721 cattaattcg cgaagcccta cgcgccgcgt tatgaggtaa aggatgattg aagcagaccg
      781 tctgatttct gccggtacca ctttgccgga agatgtagca gatcgcgcca ttcgccctaa
      841 attactggaa gagtatgttg gtcagccgca ggttcgttca cagatggaga ttttcatcaa
      901 agcagcgaaa ctgcgcggcg atgccctcga tcatttgttg atttttggtc ctccggggtt
      961 gggtaaaact acgcttgcca acattgtcgc caatgaaatg ggcgttaatt tacgcacgac
     1021 ttctggtccg gtgctggaaa aggcgggcga tttggctgcg atgctcacta accttgaacc
     1081 gcatgacgtg ctgtttattg atgagatcca ccgtttatcg ccagttgttg aagaagtgct
     1141 gtacccggca atggaagact accaactgga tatcatgatt ggtgaaggtc cggcggcacg
     1201 ctccattaaa attgatttgc cgccgtttac cctgattggt gcaaccacgc gcgcaggttc
     1261 gctgacatca ccgttgcgcg accgttttgg tattgtgcaa cgtctggagt tttatcaggt
     1321 gccggatctg caatatatcg tcagtcgcag cgcacgcttt atggggcttg agatgagtga
     1381 tgacggcgcg ctggaagttg ctcgtcgcgc tcgcggtacg ccgcgcattg ccaaccgtct
     1441 gctgcgtcga gtgcgtgatt tcgccgaagt gaagcacgat ggcaccatct cggcagatat
     1501 cgctgctcag gcgctggata tgttgaatgt cgatgctgaa ggtttcgatt atatggaccg
     1561 caaattgttg ctggcggtaa tcgataagtt ctttggtggg ccggtaggtc tggataacct
     1621 tgcggcagcc attggcgaag aacgtgaaac cattgaggat gtgctggaac cttatttgat
     1681 tcagcaaggc tttttgcagc gtacaccgcg tgggcgtatg gcgacgacgc gggcgtggaa
     1741 tcactttggc ataacgccgc cagaaatgcc gtaagtcgga ttggtcgctc gcaccgcatc
     1801 cgacattggc agtgtgcctg atgcgacgct attcgcgtct tatcaggcat ataccagaga
     1861 tagcgaaccg taggtcggaa aaggcgctcg cgccgcatcc gacattggca gtgtgcctga
     1921 tgcgacgctg ttcgcgtctt accaggccta ccagagatag cgaaccgtag gtcggataag
     1981 gcgctcgcgc cgcatccgac aaatgtgttc agcgatagat tagctggcct gctttttcat
     2041 catactgata ataaataaca gtgccgcaca tagcaccacc gacggacccg ccggcgtatc
     2101 gtaaaacgcg gaaaaggtta aaccgtcagt tactgccacc atccccacca aaacagcgac
     2161 accagccatc tgttccggcg tacgggcaaa gcgacgcgca gtagctgcag gaataatcag
     2221 caacgaagta ataatcaacg caccgacaaa tttcatcgct acaccaatcg tcaatgccgt
     2281 caccagcatc aacaacaatt tcactcgctg taatttcaca ccatcaacaa acgccagatc
     2341 cgggctaatc gtcatcgaca gcaaattgcg ccattgccag aacaaaatag ccaccacgat
     2401 gaccacgcca attgcaatag agatgagatc ttctggcgtc actgccagta aatcaccgaa
     2461 caggtaagcc atcaaatcaa cacgaatgtt agacatcaga ctaacgacca ccaggcccag
     2521 cgacagggca ctgtgcgcca taatccctaa taacgtgtcg atcgccagct gtggacgctt
     2581 ctccagccat accagaccgc ccgccagcag cagcgtaacg gcaatcaccg catagaatgg
     2641 attcacgtcc agcaacaaac caaacgcgac accaagtaat gaggcatgag ccagcgtatc
     2701 accgaaataa gacatacgac gccagactac aaacgaaccc agcggacccg cggcacaggc
     2761 gagcatgatc ccggctaacc aaccgggaaa taataattca atcatgagcg atcatttccc
     2821 cgacgcaaaa caattcgtcc ctgtaaatcg tgacgatgat tatgatgatg gcgatagata
     2881 cccagttgtt cagcaccacg aggaccaaac attgaaataa actccggatg cagggaaaca
     2941 acttccggtg tgccggaaca acaaatgtga tgattcaggc acagcacttc atcggttttt
     3001 gccatcacca gatgaagatc gtgagaaacc attaaaacgc cacaatccag ttcacgacgc
     3061 agttggtcaa taaggtcata taacgccacc tggccattta catccacgcc ttgagtgggt
     3121 tcatccagca ctaataattg cgggcgattt aacaatgctc gcgctaacag tacacgctgc
     3181 gtttcgccac cagagagctt ttgcatcggt gcgttaatca ggtgcccggc ctggacacgt
     3241 ttcagtgcag gcaaaatatc ttctttatgt gtaccagggc gtaagcgtaa aaaacggttt
     3301 acggtcagtg gcaacgtggt gtcgagatac agcttctgcg gtacatagcc gatgcgcagt
     3361 tttccgttgc gcttgataac cccttcatcg ggtgttacca gcccgagcac tacccgtacc
     3421 agtgtcgact tacctgcgcc atttggccca agtaaagtca aaatttttcc aggtttaagt
     3481 tccagcgaca catcagagag gacgcggcgt tggccaaaag aaaccgagac attttccagg
     3541 gaaaccagac ttgtcatgtt aattttagtc ttgcagtagt catgaaatgt tataatatca
     3601 cacttctcat attcattacg atgattggtc gcattatgtt acataaaaaa acgcttcttt
     3661 tcgcagcatt attcgccgct ctctggggcg gtgcaacaca ggccgcagat gccgccgttg
     3721 tcgcttcgct taaacccgtt gggttcatcg cttctgccat tgctgatggg gtaacagaaa
     3781 cggaggtttt acttcctgac ggcgcttcag aacatgatta ttcactgcgt ccatcggatg
     3841 taaaacgctt acagaacgcg gacttagtcg tttgggttgg cccggagatg gaagcgttta
     3901 tgcaaaaacc ggtaagcaaa ttaccagaag cgaagcaggt aacgattgcg cagcttgaag
     3961 atgtgaaacc gctactgatg aaaagtatta acggcgatga tgatgatcac gaccacgcgg
     4021 aaaaaagtga cgaagatcac catcacggcg atttcaacat gcatctttgg ctttccccag
     4081 agatagcgcg ggctacagcg gttgcaatcc atggaaaatt agtggaactt atgccgcaaa
     4141 gtcgagccaa acttgacgcc aacctgaagg attttgaggc acaattagcc tcaaccgaaa
     4201 cgcaggttgg taacgagctc gcgccgctca aggggaaagg ttatttcgtt tttcacgatg
     4261 cttacggcta tttcgaaaaa cagttcggac tgacaccgct tggtcatttt accgttaacc
     4321 ctgagattca acctggcgcg cagcgtttac atgaaataag aacacagttg gttgagcaaa
     4381 aagcaacctg cgtttttgct gagccacagt tcaggccagc ggtcgttgag agcgtcgcac
     4441 gagggacatc cgttcgtatg ggaacgttgg atcccttggg gacgaatatc aaactgggta
     4501 aaacaagcta ttcagaattc ctgagtcaat tagccaacca gtatgcgagc tgcctgaaag
     4561 gagattaatg aggaagtgat tacgtgcaac agatagcccg ctctgtcgcc ctggcgttta
     4621 ataatttacc gcgaccacac cgcgttatgt tggggtcgct caccgttctt actctggccg
     4681 tcgctgtctg gcggccctat gtttatcacc gcgatgccac gccaattgtc aaaaccattg
     4741 agctggaaca gaacgaaatt cgttcgctct tacctgaagc cagtgagccg attgatcaag
     4801 ctgcacaaga agatgaagcc attccccagg acgaactgga tgacaaaatc gccggtgaag
     4861 cgggcgtgca tgaatatgtt gtttccactg gcgatacgct aagcagcatt ctcaatcagt
     4921 atggtattga tatgggtgat atcacccaac tggctgcggc cgacaaagaa ttgcgtaacc
     4981 tgaaaatcgg tcaacaactc tcctggacat taaccgcgga cggcgaactg cagcgcctca
     5041 cctgggaagt gtctcgtcgt gaaacccgaa cctatgaccg tactgccgct aacggtttta
     5101 aaatgaccag cgaaatgcag caaggagagt gggttaacaa tctgctgaaa ggtaccgtcg
     5161 gggggagctt tgttgccagc gccagaaacg ccggtttgac cagcgccgaa gtgagcgcag
     5221 tgattaaagc catgcagtgg caaatggatt tccgcaaact gaaaaaaggc gatgaatttg
     5281 cggtattaat gtctcgagaa atgcttgatg gtaaacgtga gcaaagccag ctgctgggcg
     5341 tacgtttgcg ttcagaaggt aaagattatt acgctatccg cgctgaagat ggcaaattct
     5401 acgatcgtaa cggtactggt ctggcgaaag gattcttgcg attcccgacg gcgaaacagt
     5461 tccgtatctc gtctaacttt aacccgcgtc gtactaatcc ggtgaccggt cgcgttgcac
     5521 cacacagagg tgttgatttc gccatgccgc agggtacgcc agtgctttca gtgggtgacg
     5581 gtgaagtggt ggttgccaaa cgcagtggcg cagcaggtta ttatgtggct attcgtcatg
     5641 gtcgcagcta caccacgcgt tatatgcact tgcgcaagat tctggtgaaa ccggggcaga
     5701 aggtgaaacg tggcgaccgt atcgcgcttt ccggtaatac cggacgttca accgggccgc
     5761 atctgcacta tgaagtatgg ataaaccagc aggccgtaaa cccgctgacg gcaaaactgc
     5821 cgcgtaccga agggctgacc ggctccgatc gtcgcgaatt cctggcgcag gccaaagaga
     5881 ttgtgccgca gctacggttt gattaattaa catccattcg cagccggtac gcagtcagta
     5941 ccggcttttt ttatttggtg cggggcaagt tgcgccgcta cactatcacc agattgattt
     6001 ttgccttatc cgaaactgga aaagcatgga aacgaaaaaa aataatagcg aatacattcc
     6061 tgagtttgat aaatcctttc gccacccgcg ctactgggga gcatggctgg gcgtagcagc
     6121 gatggcgggt atcgctttaa cgccgccaaa gttccgtgat cccattctgg cacggctggg
     6181 acgtattgcc ggacgactgg gaaaaagctc acgccgtcgt gcgttaatca atctgtcgct
     6241 ctgctttcca gaacgtagtg aagctgaacg cgaagcgatt gttgatgaga tgtttgccac
     6301 cgcgccgcaa gcgatggcaa tgatggctga gttggcaata cgcgggccgg agaaaattca
     6361 gccgcgcgtt gactggcaag ggctggagat catcgaagag atgcggcgta ataacgagaa
     6421 agttatcttt ctagtgccgc acggttgggc cgtcgatatt cctgccatgc tgatggcctc
     6481 gcaagggcag aaaatggcag cgatgttcca taatcagggc aacccggttt ttgattatgt
     6541 ctggaacacg gtgcgtcgtc gctttggcgg tcgtctgcat gcgagaaatg acggtattaa
     6601 accattcatc cagtcggtac gtcaggggta ctggggatat tatttacccg atcaggatca
     6661 tggcccagag cacagcgaat ttgtggattt ctttgccacc tataaagcga cgttgcccgc
     6721 gattggtcgt ttgatgaaag tgtgccgtgc gcgcgttgta ccgctgtttc cgatttatga
     6781 tggcaagacg catcgtctga cgattcaggt gcgcccaccg atggatgatc tgttagaggc
     6841 ggatgatcat acgattgcgc ggcggatgaa tgaagaagtc gagatttttg ttggtccgcg
     6901 accagaacaa tacacctgga tactaaaatt gctgaaaact cgcaaaccgg gcgaaatcca
     6961 gccgtataag cgcaaagatc tttatcccat caaataaaaa agcctctcgc gaggagaggc
     7021 cttcgcctga tgataagttc aagtttgctt cagaatattc gaaatctgtt gaactatcat
     7081 tgaactgtag gccggatgtg gcgttttcgc cgcatccggc aacgtactta ctctaccgtt
     7141 aaaatacgcg tggtattagt agaacccacg gtactcatca cgtcgccctg ggtgacaatc
     7201 accaggtcac cagacatcaa gtaaccttta tcgcgcagca gattaaccgc ttcgctggca
     7261 gctgctacgc cgtcattagc gctatcaaag tgcaccggcg taacgccacg atagagagca
     7321 gtcaggttca gcgtacgttc atggcgcgac atggcgaaaa ttggtagacc agagctgata
     7381 cgggaggtca tcagcgcggt acgacccgat tcggtcatgg taatgatcgc cgtaacgcct
     7441 ttcaggtggt tagctgcgta cattgctgac atggcaatag cttcttccac attgtcgaac
     7501 tgaacgtcca gacggtgttt agaaacgttg atgctcggga ttttttccgc acccaggcaa
     7561 acgcgcgcca tggctgcaac agtttctgac ggatactgcc cagcggcagt ttctgcagac
     7621 agcatcacag cgtcagtacc atccagaacg gcgtttgcta cgtccatgac ttctgcacgc
     7681 gtcggcatcg ggttagtaat cattgactcc atcatctggg tcgccgtgat taccgctcgg
     7741 tttagctgac gcgcacgacg gatcaacgct ttctgaatgc cgaccagttc cgggtcgcca
     7801 atttccacac cgaggtcgcc acgtgcaacc attaccacgt cagaggcgag gatgatgtca
     7861 tccattgcat cctggctgca aacggcttcc gcacgttcaa ccttggcaac aattttcgca
     7921 tcacatcctg catcgcgtgc caggcgacgg gcatagttca gatcttcgcc acagcgtggg
     7981 aaggagacag ccaggtaatc tacgccaatc aacgccgcag tcttaatgtc tgctttgtct
     8041 ttttcggtca gcgcttcagc cgacaaaccg ccgccaagtt tgttgatacc tttattgttg
     8101 gagaggggac caccgacggt gacttcggtg aacactttca tgccctgaac ttccagtact
     8161 tttaactgga cgcgaccatc gtccagcagc aggatgtcac caggcacgac gtcagcaggc
     8221 aggcctttgt agttgatacc gactttttct ttgtcgcctt cacctttacc caggttggcg
     8281 tcgagcagga atttatcccc aatattgagg aaaactttgc cttctttaaa ggtggataca
     8341 cggattttgg gcccctggag gtcacccaga atagccacat gacgccccag ttttgcggca
     8401 atctcacgaa ctttatccgc gcgcattttg tgatcttcag gcgagccgtg agaaaagttc
     8461 atacgtacaa cgttggcacc cgccgcgata actttttcaa gattattatc gcgatctgtt
     8521 gctgggccta acgtggtaac gatttttgtt ctgcgaagcc ttctggacat gtaatactcc
     8581 gttgactgaa acaaccaggt gttgcttgaa catgaaatcc gaatgaaata acgccgcgat
     8641 ggcgataaaa taatctcatt cggatgtcgt ataagattag aacagtgaca gtcgctttta
     8701 gcgatcgtca cttaaattaa gtaactgctt atcaaaacgc gattccttca gcgcttcctt
     8761 gacccgcttc aagttatctc tgaattttgc accgcgtcgc aaagtaaatc ctgtcgccag
     8821 cacatctatc acggtcagct gtgcaagtcg agaaaccatg ggcatataaa tgtcagtatc
     8881 ttccggtacg tcgagggtaa ttgccagcgt tgcttcccgg gcgagcgggg tacccgcaga
     8941 ggtgagggca atcaccatag cgtcgttttc gcgtgccagc tgcgccagct cgaccagatt
     9001 ttttgttctt ccggtgtgag aaatcagcac caccacgtct ccgtcgctac aattcataca
     9061 actcatgcgt tgcagcacaa tatcatcgga gtacaccacc ggaacattaa aacgaaagaa
     9121 cttattcatc gcatcgtggg caacggcggc tgaagagcct aatccgaaaa aggcgatttt
     9181 ttttgcctga gtgagcaagt cgacggcgcg gttgatggca gatttatcca gtgaatgacg
     9241 gacatgatcg agcgttgcca ttgcggactc aaatattttc cctgtgtatg attcaacgct
     9301 gtcatcttca ttgacgttgc gattaacata gggagtgcca ttcgccagac tctgtgccag
     9361 atgaagttta aaatcaggaa aaccgcgcgt gtccatgctg cgacagaaac gattcaccgt
     9421 cggttcgcta acattggctt ccagtgccag agcagcaata ctcgaatgga tcgcgttatc
     9481 gggcgaagcc agaatgacct cggcaacttt gcgctctgat ttgctcaaat gttccagctg
     9541 agactggatt ttttccagca tattcatgat gtaaagagac tcacgggtaa tgacgatttc
     9601 cgcactgaaa gaaatcgaaa tgcagttttg tcagatatta cgcctgtgtg ctgtgttaat
     9661 gacaaaagca gataaaaaag ttgttatttt ttttcataac atgatcagtg tcagattttt
     9721 acccaatgga aaacgatgat ttttttatca gttttgccgc actttgcgcg cttttaccgt
     9781 aatcgcacgg gtggataagc gtttacagtt ttcgcaagct cgtaaaagca gtacagtgca
     9841 ccgtaagaaa attacaagta taccctggct taagtaccgg gttagttaac ttaaggagaa
     9901 tgacatggcg gtaacgcaaa cagcccaggt ctgtgacttg gtcattttcg gcgcgaaagg
     9961 cgaccttgcg cgtcgtaaat tgctgccttc cctgtatcaa ctggaaaaag ccggtcagct
    10021 caacccggac acccggatta tcggcgtagg gcgtgctgac tgggataaag cggcatatac
    10081 caaagttgtc cgcgaggcgc tcgaaacttt catgaaagaa accattgatg aaggtttatg
    10141 ggacaccctg agcgcacgtc tggatttttg taatctcgat gtcaatgaca ctgctgcatt
    10201 cagccgtctc ggcgcgatgc tggatcaaaa aaatcgtatc accattaact actttgccat
    10261 gccgcccagc acttttggcg caatttgcaa agggcttggc gaggcaaaac tgaatgctaa
    10321 accggcacgc gtagtcatgg agaaaccgct ggggacgtcg ctggcgacct cgcaggaaat
    10381 caatgatcag gttggcgaat acttcgagga gtgccaggtt taccgtatcg accactatct
    10441 tggtaaagaa acggtgctga acctgttggc gctgcgtttt gctaactccc tgtttgtgaa
    10501 taactgggac aatcgcacca ttgatcatgt tgagattacc gtggcagaag aagtggggat
    10561 cgaagggcgc tggggctatt ttgataaagc cggtcagatg cgcgacatga tccagaacca
    10621 cctgctgcaa attctttgca tgattgcgat gtctccgccg tctgacctga gcgcagacag
    10681 catccgcgat gaaaaagtga aagtactgaa gtctctgcgc cgcatcgacc gctccaacgt
    10741 acgcgaaaaa accgtacgcg ggcaatatac tgcgggcttc gcccagggca aaaaagtgcc
    10801 gggatatctg gaagaagagg gcgcgaacaa gagcagcaat acagaaacct tcgtggcgat
    10861 ccgcgtcgac attgataact ggcgctgggc cggtgtgcca ttctacctgc gtactggtaa
    10921 acgtctgccg accaaatgtt ctgaagtcgt ggtctatttc aaaacacctg aactgaatct
    10981 gtttaaagag tcgtggcagg atctgccgca gaataaactg actatccgtc tgcaacctga
    11041 tgaaggcgtg gatatccagg tactgaataa agttcctggc cttgaccaca aacataacct
    11101 gcaaatcacc aagctggatc tgagctattc agaaaccttt aatcagacgc atctggcgga
    11161 tgcctatgaa cgtttgctgc tggaaaccat gcgtggtatt caggcactgt ttgtacgtcg
    11221 cgatgaagtg gaagaagcct ggaaatgggt agactccatt actgaggcgt gggcgatgga
    11281 caatgatgcg ccgaaaccgt atcaggccgg aacctgggga cccgttgcct cggtggcgat
    11341 gattacccgt gatggtcgtt cctggaatga gtttgagtaa tatctgcgct tatcctttat
    11401 ggttatttta ccggtaacat gatcttgcac agattgtaga acaattttta cactttcagg
    11461 cctcgtgcgg attcacccac gaggcttttt ttattacact gactgaaacg tttttgccct
    11521 atgagctccg gttacaggcg tttcactcat aaatcctctg aatgaaacgc gttgtgaatc
    11581 atcctgctct gacaactcaa tttcaggagc ctttatgaat ccacaattgt tacgcgtaac
    11641 aaatcgaatc attgaacgtt cgcgcgagac tcgctctgct tatctcgccc ggatagaaca
    11701 agcgaaaacg tcgaccgttc atcgttcgca gttggcatgc ggtaacctgg cacacggttt
    11761 cgctgcctgc cagccagaag acaaagcctc tttgaaaagc atgttgcgta acaatatcgc
    11821 catcatcacc tcctataacg acatgctctc cgctcaccag ccttatgaac actatccaga
    11881 aatcattcgt aaagccctgc atgaagcgaa tgcggttggt caggttgcgg gcggtgttcc
    11941 ggcgatgtgt gatggtgtca cccaggggca ggatggaatg gaattgtcgc tgctaagccg
    12001 cgaagtgata gcgatgtctg cggctgtcgg gctgtcccat aacatgtttg atggtgctct
    12061 gttcctcggg gtgtgcgaca agattgtccc gggtctgacg atggcagccc tgtcgtttgg
    12121 tcatttgcct gcggtgtttg tgccgtctgg accgatggca agcggtttgc caaataaaga
    12181 aaaagtgcgt attcgccagc tttatgccga aggtaaagtg gaccgcatgg ccttactgga
    12241 gtcagaagcc gcgtcttacc atgcgccggg aacatgtact ttctacggta ctgccaacac
    12301 caaccagatg gtggtggagt ttatggggat gcagttgcca ggctcttctt ttgttcatcc
    12361 ggattctccg ctgcgcgatg ctttgaccgc tgccgctgcg cgtcaggtta cacgcatgac
    12421 cggtaatggt aatgaatgga tgccgatcgg taagatgatc gatgagaaag tggtggtgaa
    12481 cggtatcgtt gcactgctgg cgaccggtgg ttccactaac cacaccatgc acctggtggc
    12541 gatggcgcgc gcggccggta ttcagattaa ctgggatgac ttctctgacc tttctgatgt
    12601 tgtaccgctg atggcacgtc tctacccgaa cggtccggcc gatattaacc acttccaggc
    12661 ggcaggtggc gtaccggttc tggtgcgtga actgctcaaa gcaggcctgc tgcatgaaga
    12721 tgtcaatacg gtggcaggct ttggtctgtc tcgttatacc cttgaaccat ggctgaataa
    12781 tggtgaactg gactggcggg aaggggcgga aaaatcactc gacagcaatg tgatcgcttc
    12841 cttcgaacaa cctttctctc atcatggtgg gacaaaagtg ttaagcggta acctgggccg
    12901 tgcggttatg aaaacctctg ccgtgccggt tgagaaccag gtgattgaag cgccagcggt
    12961 tgtttttgaa agccagcatg acgttatgcc ggcctttgaa gcgggtttgc tggaccgcga
    13021 ttgtgtcgtt gttgtccgtc atcaggggcc aaaagcgaac ggaatgccag aattacataa
    13081 actcatgccg ccacttggtg tattattgga ccggtgtttc aaaattgcgt tagttaccga
    13141 tggacgactc tccggcgctt caggtaaagt gccgtcagct atccacgtaa caccagaagc
    13201 ttacgatggc gggctgctgg caaaagtgcg cgacggggac atcattcgtg tgaatggaca
    13261 gacaggcgaa ctgacgctgc tggtagacga agcggaattg gctgctcgcg aaccacacat
    13321 tcctgacctg agcgcgtcac gcgtgggaac aggacgtgaa ttattcagcg ccttgcgtga
    13381 aaaactgtcc ggtgccgaac agggcgcaac ctgtatcact ttttaagacg acaaatttgt
    13441 aatcaggcga gagaaaactc tgatgaaaaa ctggaaaaca agtgcagaat caatcctgac
    13501 caccggcccg gttgtaccgg ttatcgtggt aaaaaaactg gaacacgcgg tgccgatggc
    13561 aaaagcgttg gttgctggtg gggtgcgcgt tctggaagtg actctgcgta ccgagtgtgc
    13621 agttgacgct atccgtgcta tcgccaaaga agtgcctgaa gcgattgtgg gtgccggtac
    13681 ggtgctgaat ccacagcagc tggcagaagt cactgaagcg ggtgcacagt tcgcaattag
    13741 cccgggtctg accgagccgc tgctgaaagc tgctaccgaa gggactattc cgctgattcc
    13801 ggggatcagc actgtttccg aactgatgct gggtatggac tacggtttga aagagttcaa
    13861 attcttcccg gctgaagcta acggcggcgt gaaagccctg caggcgatcg cgggtccgtt
    13921 ctcccaggtc cgtttctgcc cgacgggtgg tatttctccg gctaactacc gtgactacct
    13981 ggcgctgaaa agcgtgctgt gcatcggtgg ttcctggctg gttccggcag atgcgctgga
    14041 agcgggcgat tacgaccgca ttactaagct ggcgcgtgaa gctgtagaag gcgctaagct
    14101 gtaaaagtca aaatgcccga tcgaggatcg ggcatttttg tagcgtttat ttgccgggtt
    14161 taaccctgta cttttacctg tccggcggcg tgcttcgcgc gttcaatggc gtcaacaaca
    14221 ctctctgcag tagccagtgc cacccccaga cgacggctgc catcaatttc cggcttacca
    14281 aataaacgaa tctgcaaatc tgcgcctacg gcattctgca cattatcaaa cgtgacattc
    14341 tgactggtca gttgtggcag aataacggca gaagctgcag gaccatactg acggatcccg
    14401 ccaaccggaa gtccgaggaa ggcacgtaca tgcagggcaa actctgagag atcttgagaa
    14461 attaacgtca ccatcccggt atcatgtgga cgaggggaga cctcactgaa aatcacctca
    14521 tcaccacaga caaacagctc gacaccaaac agaccataac cgcccagtgc cagcaccact
    14581 ttacgggcaa tctcctgcgc acgttcaagg gcaagcgggc tcatttgctg tggttgccag
    14641 gattcacagt agtcgccatc ttcctggcga tgacctactg gtgcacagaa atggacgcca
    14701 tccaccgcgc tgacggttag cagggtaatt tcgaagtcaa acttaacgac gccttcaaca
    14761 attacgcggc ccgctccggc gcgaccgcct tgctgagcgt attcccatgc ctgagcaagt
    14821 tgctctgcag agcgaataaa cgtctgcccc ttgccggaag agctcatcac cggttttaca
    14881 atgcagggat agccaatggc ggcaaccgcc tcgcggaaaa ggctttcgct atcggcaaaa
    14941 cgataagtgg aagtgggcag ctgcagctct tctgccgcca ggcgacggat accctcgcga
    15001 ttcatcgtta atttcgttgc gcgagcgcag gggacaacat tcagtccttc ctcttcaagt
    15061 tggatcagca tatcggtggc aatagcttcg atctccggca cgatataatg tggtttttcc
    15121 agttcaacca cacggcgtaa tgcatcacca tcaagcatat taatgacatg ggagcgatgc
    15181 gcgacatgca tggctggtgc gtcggcatag cgatcgacgg caatcacctc tacgccgaga
    15241 cgctgacact cgattgccac ttctttaccc agttcaccgg agcctaataa catcacgcga
    15301 gttgctgccg gacgcagcgc agtgcctaat aacgtcataa cgaatatccc ctgattaatt
    15361 ccgcgcgcag tataaacgaa aacgtttgcg tgtgtcttta ttgctggtgt tgatctctca
    15421 accgaaaaga aatatactgt atagaatcac agttattatg agaggctgca aatggcggtt
    15481 gaagttaaat acgtagtcat tcgtgagggc gaagagaaaa tgtcgtttac cagcaaaaag
    15541 gaagccgatg cttatgacaa aatgcttgat acggcggatc tgcttgacac ctggctgaca
    15601 aattctccag tgcaaatgga agacgagcaa cgtgaagccc tttcgctatg gctggcagaa
    15661 caaaaagatg tgctgagcac cattctgaaa accggcaaat taccgtcccc acaggtcgtc
    15721 ggtgctgaat ccgaagagga agatgcctca cacgcggcct gaagcaactt gcgctcctga
    15781 caatttgtac catgctttct cctgaatcat tgtgctatgg agaaaaacat gaaaaaaaga
    15841 ggggcgtttt tagggctgtt gttggtttct gcctgcgcat cagttttcgc tgccaataat
    15901 gaaaccagca agtcggtcac tttcccaaag tgtgaaggtc tggatgctgc cggaattgcc
    15961 gcgagcgtaa aacgtgatta tcaacaaaat cgcgtggcgc gctgggcaga tgatcaaaaa
    16021 attgtcggtc aggccgatcc cgtggcatgg gtcagtttgc aggacattca gggtaaagat
    16081 gataaatggt cagtaccgct aaccgtacgt ggtaaaagtg ccgatattca ttaccaggtc
    16141 agcgtggact gcaaagcggg aatggcggaa tatcagcggc gttaatgggt tctgataatt
    16201 cgattgaccg ttggcggatg cggcgcgagt gccttgtccg ccccagaacg caccacaatc
    16261 gtaggcctga gaagacgcaa cagcgtcgca tcgggcaatc agcatcaaac aatgccactc
    16321 aaaacgacaa actactttca cctaaaatac ccttatcacc tgatatacag taaatacctt
    16381 acctggcgcg aatttctttg aataacggcc taccgccccg cttatttgcg catcttttgt
    16441 ctcgatatac attttctgac atcctcatcc tctatgctac aggcactaaa aatggttatt
    16501 gaggagcaaa tatggctaac tggttaaatc aactgcaatc cctgcttggg caaagcagtt
    16561 cttctacctc ctcgtctgcg gatcagggat tgggcaaact tttagtgccg ggcgcattag
    16621 gcggactggc tggactgctg gtcgcaaata aatcagcacg taaacttctt acgaaatatg
    16681 gcaccaacgc gttgctgatt ggcggcggag cggtagcggg tacggtgctg tggaataaat
    16741 acaaagataa aattcgcgcg gcgcatcagg acgaaccgca gtttggctcg caaagtacgc
    16801 cgctggatga gcgtacggaa cgtttgatcc ttgcgctagt ctttgccgct aaaagtgatg
    16861 gtcatattga tgccaaagaa cgtgcggcaa tcgaccagca attgcgtgaa gccggcgtgg
    16921 aagagcaggg gcgggtactc attgagcagg caatcgaaca accgctggat ccacaacgcc
    16981 tggctaccgg ggtccgcaat gaagaagagg cgctggaaat ctatttcctg agctgcgcgg
    17041 ctattgatat tgaccatttt atggaacgca gttacctgaa tgcactgggt gacgcgctga
    17101 aaatccccca ggatgttcgt gaaggcattg aacgcgatct cgaacagcaa aaacgcactt
    17161 tggcggaata attccgctct ctggatagcc tgaagttgac tatttcgggc tatccagaac
    17221 agcacagcca tttgaacagg ctgattttct ctgtctgcat aacattcgat attcgcgcca
    17281 ttacctgtca tcatctaagc aatgactgcc ctgtttcgct tgcatccccg gtgagttttg
    17341 ccacccttat aagatgtttc aaccagaaag aacaataaca tgctaccaaa agccgcccgc
    17401 attccccacg ccatgacgct tcatggcgat acgcgcatcg ataattacta ctggctgcgg
    17461 gacgatacgc gttctcagcc ggaagtcctg gactacctgc aacaagaaaa tagttacggt
    17521 catcgggtga tggcctcaca acaagccttg caggatcgca tcttaaagga aatcatcgac
    17581 cgcattccgc aacgagaagt ttctgcgccc tacatcaaaa atggctaccg ctaccggcat
    17641 atttatgaac caggctgtga atatgctatc taccagcgtc aatcggcgtt cagtgaagag
    17701 tgggacgagt gggaaatatt gctcgatgcc aacaagcgcg cggctcatag tgagttttat
    17761 tcgatgggcg gaatggcgat tacgcccgat aacaccatta tggcgctggc agaagatttt
    17821 ctttcccgac gccagtacgg cattcgtttt cgtaatctgg aaacaggtaa ctggtacccg
    17881 gaactgctgg ataacgttga acccagcttt gtctgggcaa atgactcctg gactttctac
    17941 tatgttcgca agcatccggt gacgctgctg ccttatcagg tctggcgtca cgccatcggt
    18001 acgccagcat cgcaagataa actgatttac gaagaaaaag acgataccta ttacgtcagc
    18061 ctgcataaaa cgacctcaaa gcactatgta gtcattcatt tggccagcgc caccaccagt
    18121 gaagttcgcc tgctggacgc ggaaatggca gatgccgagc cgtttgtttt tctgccgcgc
    18181 cgcaaagatc acgaatacag ccttgatcac taccagcatc gtttttatct gcgttccaac
    18241 cgccacggca aaaactttgg cttataccgt acccgtatgc gtgatgagca acagtgggaa
    18301 gagttaattc cgccacgcga aaacatcatg ctggaagggt ttacgctgtt taccgactgg
    18361 ctggtggttg aagagcgtca gcgcgggtta accagtttgc gccaaattaa ccgcaagacc
    18421 cgggaagtca ttggtattgc ctttgatgat ccagcctatg tgacctggat tgcctacaat
    18481 ccagaacctg aaaccgcgcg attgcgttat ggttattctt ccatgaccac accagacact
    18541 ttgtttgaac tggatatgga taccggtgag cgtcgtgtat taaaacaaac agaagttcct
    18601 ggttttgatg cggcgaatta ccgcagtgaa cacctgtgga tagtcgcccg tgatggtgtc
    18661 gaagttccgg tttcgttggt ctaccatcgc aatcattttc gcaaaggaca caacccgctg
    18721 ctggtgtatg gctacggttc ttacggcgca agtattgatg ccgatttcag ttttagccgc
    18781 ttgagtttgt tagatcgtgg ctttgtctac gccattgtcc atgttcgcgg cggcggtgag
    18841 ctggggcaac aatggtacga agacggtaaa tttctgaaga agaaaaatac gtttaatgat
    18901 tatcttgatg cctgcgatgc attgttaaaa ctgggctatg gctctccttc gctttgttat
    18961 gcgatgggcg ggagtgcggg gggcatgttg atgggcgttg caattaatca acgcccggaa
    19021 ttattccacg gcgttatcgc ccaggtaccg tttgttgatg ttgtaacaac gatgcttgat
    19081 gaatcaattc ctcttaccac tggtgagttt gaagagtggg ggaatccgca ggatctgcaa
    19141 tattacgagt atatgaaaag ctacagcccg tatgacaacg tcaccgcaca ggcttatcca
    19201 catttactgg taacgaccgg tttacacgat tctcaggtgc aatattggga accggcaaaa
    19261 tgggtcgcta agttgcgcga gctgaaaacc gataaccatc ttttattgct ctgtaccgac
    19321 atggactcag gccatggcgg taaatctggt cgctttaaat cgtacgaagg cgtagcgatg
    19381 gaatatgctt ttctggtcgc gctggcgcag ggaacattac ccgctaggcc tgcggattag
    19441 gtattttcca gataatgttt cagtgttaaa cgcagctccg ggctcatgct gtccaggtta
    19501 ttaaacaacc agcgcagata gcccggatcg cgttcggcaa cgtcggaaac cgctttgcca
    19561 cggtatttgc caaaggtgaa ggtcgtcatc aacgacggac gtccggttat atcggccatc
    19621 tgttctgccg tccagccgga ggtgttcata atatcgataa gcaacgcggc ggtgatataa
    19681 cagtcataca acgcgcggtg atgatgcagg cccggcggcg tctgtacatt gagcttccgt
    19741 gttttatata acgccatatt gctgtacttg atccctggcc acaaacgacg ggccagtttc
    19801 atagtgcaaa tccactcacc gggcatctca ggcagtacgc ggcggtcaaa gctggcgtta
    19861 tgcgcgacat accattcact accgtaatag tgtgggatca catcttcaat ccacggttta
    19921 tcggcgacca tggcttcggt gatgcgatga atcgccatcg cttgtggact aataggacga
    19981 tcggggtgca ccaggtggct catggggttg acgattttcc cgtcaatgac atcaacagag
    20041 gcaatctcaa cgatccctcc ctgcaaaccg caggtttctg tatcgataat gcgcaacatg
    20101 gcatgctcca ggccgaaaaa gcctagcgta atggaatgat atcgccttgc caaccctgtg
    20161 atgaacgctg accgactaac aataatgaac cacgatcggc gcgaacgatg agacgaccat
    20221 gttcatccca tagtgcactg ttaccacggg catttgccat cagcacagca atcgagtact
    20281 gatgtgaaaa aaattgtaag cgacgggccg atgccagcag gtcgggttct cccagacatt
    20341 gccctgtcgt aaacagtgaa caggtcgggt ccatatccat accttgcggt tgttcatcga
    20401 ccaccgtgat ggttctggaa cgacggccca gacatgcacc atgactctga tggtagatcc
    20461 caggcgtttt tcgccacggt gcgaacaccg caattccacg aataaagcga tcgttatatt
    20521 caacgggaag gccggcaatg atggtcattc gccaggtcgt tgcggcataa caaagcgggt
    20581 ctaacagtga gagatcgggt ggggcaggaa gggcacgtcg cgaataatca caccccagta
    20641 aagaaagcga tggaaaaact aacagctgac attgttgcct ggcggcggcc ctcacaaact
    20701 ccagatgatg agctacctgt tcggtgagtg aggttttacg aggctcatat tgcgctgctg
    20761 caactttcca gaacgacata atgacttcct tttcaaaatg actgcaaatt atgggtgcgg
    20821 tttagaaaat actcataatt tagcaggcgt tatgtaagaa agtgtaactc tgtataagtt
    20881 ttatttaagt ttgggctcgt aaggtaaacg tgacagattg accgaagcca aacggtgggc
    20941 aataagccgc tcgcgaaacc agctgcgcaa atgttcaggc tgttcacgtt caaccgcttc
    21001 agcgatcact ggcatattgt agcgttcttt aaatgccacc ccggccgccg ccaaatcgac
    21061 attcacttta tccatttctg tttgatccag tttagccaga ttcttcagca ttcttaatct
    21121 cctcatcatt cgcgggcaaa agttactaag agtgagcctg gatggcaagc cggaattgat
    21181 gatcctcgac tcagacatta gacaaggtta tgctctgtaa aaaagttatt acaaaaagga
    21241 atagataata tggcttcaac tgcacgctcc cttcgctacg cgctggcaat cctgaccact
    21301 tcgttggtga caccttccgt ctgggcacat gcgcatttaa cgcatcagta tcctgcggca
    21361 aacgcgcaag tgacagctgc accgcaggca atcactttaa acttctcgga aggtgttgaa
    21421 acaggattca gtggtgcaaa aatcacgggg ccaaaaaacg aaaatattaa aacattaccg
    21481 gcgaagcgaa atgagcagga ccaaaagcga ctgattgttc cactggccga ttcgctgaaa
    21541 cccggaacct acaccgttga ctggcatgtt gtttcggtgg acgggcataa aacgaaagga
    21601 cactacacct ttagcgtgaa ataaatcatg ctggcgttta cctggatcgc gctgcgattt
    21661 atccatttca cctcgctgat gctggtcttt ggcttcgcaa tgtacggtgc ctggctggca
    21721 cccttaacga ttcgtcgctt gctgacgaag cgttttctgc gtttacaaca gcacgctgcc
    21781 gtctggagtt tgatcagtgc cactgcaatg ctcgccgttc agggcgggtt gatgggaacg
    21841 ggatggaaag atgtattttc gccaaacatt tggcaggctg ttttgcaaac gcagtttggt
    21901 ggcgtctggt tatggcaaat tgttctcgcc ctcgtaacgt tgatcgtcgc ccttatgcaa
    21961 ccgcggaata tgccacgttt gctgtttatg ctcactaccg cgcaatttat tctgctggcg
    22021 ggagtggggc atgcgacgct gaatgaaggg gtaacggcga aaatccatca gactaatcac
    22081 gcgattcacc tgatttgtgc ggcggcgtgg tttgggggat tactgccggt gctctggtgt
    22141 atgcaactca tcaaaggccg ttggcgacac caggctattc aggcgctgat gcgtttttcc
    22201 tggtgcgggc attttgcggt gatcggcgta ctggcaagcg gcgtgcttaa tgcattgttg
    22261 attaccggat ttccccccac gcttacgact tactggggcc aactcttatt gctcaaagcc
    22321 atcctggtga tgattatggt ggtcattgcg ctggctaatc gttatgttct tgtaccgcgc
    22381 atgaggcagg atgaagatcg tgcggcaccc tggtttgtgt ggatgactaa actcgaatgg
    22441 gcgataggtg cggtagtgct gctgatcatc agcctgcttg cgactctcga acctttttga
    22501 tggattacga aaatgatgaa aaaaagtatt ctggcgtttc tgttactcac cagttctgca
    22561 gcggcgctgg ctgcaccgca ggtgattacc gtcagccgtt ttgaagtggg taaagacaaa
    22621 tgggcgttta atcgcgaaga ggtgatgctg acttgccgac cgggtaatgc tttgtatgtc
    22681 atcaacccaa gtaccctcgt gcagtatcct ttaaacgatg tcgcacagaa ggaagttgcc
    22741 agtgggaaga ctaaagccca acccatttcg gtgattcaga ttgatgatcc caacaatccc
    22801 ggcgaaaaaa tgagtctggc accgtttata gaacgagctg aaaaactctg ttaattacct
    22861 aaaatagcct tttgatttcc aataaaaaaa ccgcctcagt tctttcacca gaacgggcgg
    22921 tttttaacat ttaagctgat gaccaccgcg ctttttattg accattttgc acgcaaactg
    22981 gaaaacctgg cgtcgtcatc tattcttaaa gggcaaggca actaagcctg cattaatgcc
    23041 aacttttagc gcacggctct ctcccaagag ccatttccct ggaccgaata caggaatcgt
    23101 gttcggtctc tttttatctg ttaaaagcca gaagcatttc cttcgctgac tttatagtca
    23161 accataacac acactctact gtctgagtcc agcgtttttt aacattcttg ttaagattat
    23221 gtgatcttta gcgcgggagg aaaatattga tgaaacagcc tgcgcccgtt tatcagagaa
    23281 ttgcgggtca tcaatggcga catatctggc tttctggcga tatacacggt tgtcttgagc
    23341 agttgcgccg caaattatgg cattgtcgtt ttgatccgtg gcgagattta cttatctcag
    23401 tgggagacgt tatcgatcgt gggccgcaaa gtttacgttg tctgcagtta ctggaacaac
    23461 attgggttcg tgcggtaaga ggcaatcatg aacagatggc gatggatgcg ctggcatccc
    23521 agcagatgtc tttgtggttg atgaatggcg gcgactggtt tattgcgctg gcagataatc
    23581 aacagaaaca agcgaaaacg gcgctggaaa aatgtcagca tttgcccttt attcttgaag
    23641 tacacagccg caccggcaaa catgttattg ctcatgccga ttatccagat gatgtttatg
    23701 aatggcaaaa ggacgttgat ttgcatcagg tcttgtggag ccgctcgcga ttaagtgaac
    23761 gccaaaaagg gcagggaatt acaggtgctg atcatttctg gtttggtcat acaccgttgc
    23821 gccatcgcgt ggatattggc aacctgcatt atattgatac cggcgctgtc tttgggggcg
    23881 aactgacttt cgtgcaattg caataattaa aaatcaccat actcctgtgc tggtcgccag
    23941 aaaccatcta taaaatcctc aatcggaaaa caaccgccat gacggatccg ttgatcgctc
    24001 atagaataaa gacactgctg ttccgtgttg tagacatcca caacaatatc ttcacaaccg
    24061 ccatccaggt agcaaacaaa aagtaccagc gcgaacattt catccccgaa gtgtggtgcc
    24121 gtaccgttaa gtttaggaga gattttacaa cgggggaata accaggacaa ataacccgcc
    24181 atgccgacgg gttctttttg gatcaggcaa gacgcataat ccaggcatca gattgttgat
    24241 cgtaacgggt acggtaaagg acagagtgtt cgccatttag cagggcaggg atactggttt
    24301 cagcatccca ggcatccagt tgcatacata aatcggggtc tgatttacaa ggaatggtta
    24361 acgttcgatc acccggcgat tcaccgtgta attcgccgtc gtcgatttca aaagcgccta
    24421 tgcgcacact ggttttcatc ttcgtgctct ccatttagct gccggttgtg gtacgaccag
    24481 tccgatcgca ccatttttca gcctagttga ggccgccgaa tgtgccagtc aaaaagtgcg
    24541 ctttttttgt gcgctgtcat gcgttactgg taaaaagttt cccgtcccgc accagttcac
    24601 gcgggtagct gtttttcagt cgcgaaccaa tccgtttggc taaaccaatc ggctgatgct
    24661 ggaaagtaac caacacgtca tccgccactg gcgcggcttg cgggtaaaca tcgcgcccgc
    24721 gataccactc ctccgcttct tgcggtgtca gttcaaaagc gttctcgttg tcgggggagg
    24781 caagggcaat aaccgcttca tgctgccagc gataaccttt gttgtgcgtt tcggcaagtt
    24841 taatccccag gcgagaaaat cggactttac cgatcagggc ttcaatgcct accggaaaca
    24901 accacacttc tttgtcgcgc tgccagagtc gcaggtttcc atcccagttt aagccaacac
    24961 ttgcagccgc ctgacgaatt tgtgcggctt cgcgatcttt caccgggctg aacgggaaat
    25021 tgcccacttt gtattttggg gtgggtaagg cgggaatggc ctgagtttta cgcagacgag
    25081 caacgaagaa gccttcgcag tcgtaaattt gtgggaaaac atgcaaaaag ccttcttcgg
    25141 tcagcgcttt gtttgcacca gggaagagat cgccaagtgg taaaaactct actgcgtcgg
    25201 ggtaagtctc tttaagccac aggcaaacgg attcgttttc ttcccggttt aaggtacagg
    25261 tcgagtaaac cagtgtaccg ccagagcgta atgcatgaaa ggcactgtcg atcagttccc
    25321 gttgggtagc tgcgatttcc tgattgcttt ctggtgacca attttttagc gcatcgggat
    25381 ctttacgcac cacgccttcg ccggagcagg gagcgtccag caaaatggca tcgaacattt
    25441 ctggcaatgc cgcaccaaac acgcggccat caaaatgtgt gagcgcaaca ttactgatac
    25501 cacagcggct gatattggca tgtaacactt ttacccgact ggcggaaaac tcattggcaa
    25561 ggattgcccc ttcgttattc atccgcgcgg caatttgcgt cgttttggag cctggcgcag
    25621 cagcgacatc catcacccgc tgtggtgcat taccgtcagc aaacaaggcg gcgacgggca
    25681 acattgaact ggcttcctga atataaaaca ggccacttaa atgctcggcg gtactaccca
    25741 atggcaatgc atcttcatcg tcgcgttcaa tccagaaacc ttcttcacac cacggaattg
    25801 gcgtaagcgt ccagccataa ggagcggtta atcgcaggaa atcagcaacg gaggttttca
    25861 gcgtattaac gcgaatgctg cggcgcaacg ggcgctgaca ggcggcaaga aaatcatcaa
    25921 aagagagcgt cgaaggcatt gcttcgcgca tttgcgtcag aaaggcgtcc gggaaataaa
    25981 cggtgtgttg ggccacgagc atataccact ggcaaaaaaa acaggcgcgc agtttagcat
    26041 aaacgctccg gcgcaggcac gccggagcag tgggcattat ttgggaagcg cagttcccca
    26101 ttcacgccac tctttcggtt cactttcctg caacaggaag tgcttgcctt cctgggcttt
    26161 cggtgccagt ggcgtacccg gaggcgtggc gaaggcgatg ccgccacgga taaactggtt
    26221 aaaggtgccg gttttcacta cgccgcccgt cagaccaaag tccagactgt aacccgatgc
    26281 caaccagaag acggaattgt tacgcaccag gtgttgatag cgtttactga tgcgcatcgc
    26341 aatcatcacg cgatctgaca atgtccccag cgtcattccg gtaaccgtac cgacttccag
    26401 accacggaac agcacaggcg taccgatgcc taacgaacag gcttccggcg cttcaacaat
    26461 aatgcttaaa ccatccaggt aacgcgaatc agtaatggtg gcctcttgta attcaaagtc
    26521 gcgacgagga ttgccacggc ctggttcgac gttgatatac ggctggagga tagtatcaag
    26581 atgctcaacg ccagctgccg aaatttgcgg tgtgaccact gagaagcgcg taccaccgcg
    26641 agcaaaggtc tggacatatt ccggatagag caccgccttt gcctgtactt cattgcgcgc
    26701 ggtaatcaga tccagcgtct ggatttgccc gatatcaata ccgagatagc gaattggcat
    26761 cccgaccgcc agttttccgg catcgaaagc atgaagcgta atctgcccgc caaccgcacg
    26821 ggccgctgtt tcggaagcat acagaatacg cttatcgcct ttacgctgac tggcgctggc
    26881 accgctgagg ttatcgaagc taatggctcc ctttaatgct ctggagagtg gggatgcctg
    26941 tacggtcagg ccactaccat tcagctgaac tttcgccccg ccttctgccc agaacacgct
    27001 attgttggtc agaaggttgc gatactccgg cttaatatgc agatcgatat caaacgcgtt
    27061 agctcgcgga cgcacggtaa taacttcacc aacttcaaat ttacggtaca gcactaccga
    27121 tcctgcctgc acatccggca gcgtctctgc actcaaactc acggttgtgg tgggtaaatc
    27181 gctaaggctg ttctccagcg ctttttccag attggcatac agtggatagc tggctttcat
    27241 ctcaccttta tcgcccggca gaatacgtat cccgccattg atccattctg aggcgctggc
    27301 accgagaaac tcaacgccat ccagccccac cttcacatcg acacggctgt tgacgacaaa
    27361 tttgctatcg ccttttacca gttcgcgatg ctgaggctca atggcgacgg taaaggtaac
    27421 gcctttgctg gtgagtttac gatcaataac ctggcctacc tgcacgccgt gaagaatgag
    27481 cggctgacca gcatcaatac cgtaactttc cggtgctgtc agggtcagtg tcagaacatc
    27541 aggttcatgc agcagtgctt tttcgcctgg cacaacaacg aactctttgc gtggctcgcc
    27601 atcgccaggc accaactcaa aggtttttcc ggtcagcagg gcgctgagat tggcatcgct
    27661 aagggataat ttcgggttgc gtaattcgat gcgggtattt tcccgtaaca gcgtaacgac
    27721 gctgggatca acggtcattt ctccggtgac tttaccacca ggatttaaat ccagtttagt
    27781 cagctgtccg acttccagcc cctgatacat taacggcgtc gagtcggcgg ttaatccggc
    27841 cccactcggc agttccagtt ttattattac gccacgctgg ctgtgggcca gatcttcata
    27901 cagaccaaag gtatcttccg cctcggcagg tttcgactct tctggtgaat cgaaggcaat
    27961 cgcaccgtta accagtgccg ccagactttc cagtttcacc ttcgcgccac tgatactgac
    28021 gttggcatca acgcctgaaa cgttccagaa acggctacct tttttcacca ggtcggtaaa
    28081 acgccgctcg atcaggacat caatcaccac gccttgttta ttgggattga tggcatagtc
    28141 gtagactttt cccaccggga tcttgcggaa atagaccaat gaaccgctgt tcagcgaacc
    28201 gagatcggga gcttgcaggt ggatcatcag atcgccgtta tccagccgat acttcggttg
    28261 ggtatcgagc gcgacaaagt gatcctgctc tttaccttta cctggcatca tgccgatata
    28321 gttaccaccg acgagggcgt ccagcccgga gacacctgcc aacgatgctt ttggcgtcac
    28381 cagccagaac tgagtctctt cgcgcagcgc atctttcata tcggacttga tgctgacctt
    28441 gacttcaatc ttacgaagat cgtcgctgag gctgatatcc tgcactgttc cgacttcaac
    28501 gccctgataa cgaacaggcg tacggcccgg gacaatacca tccgccgaca taaagtcgat
    28561 ggtgacggta ttaccccggt cctgataact gtcccaaatc agccaaccgg caatcattag
    28621 cgcgatgaac ggcagcagcc agaaaggtga gatacggcgt ttatttttaa tctgcgcttc
    28681 agtcgtcgaa gcgggcgttt cctgactcat gtgcatccca aagtaagcgg ctgtccagcc
    28741 attccacagc aagaatagtc aaaattaccg ctgcgccgaa ataaaacgca gccggtccca
    28801 tagtaaaagc gaggatctga tcgcgattaa tcagcgacat ggttaaagat atgacaaaca
    28861 ggtcgagcat cgaccagcga ccaatccagg tcaccatccg cagtaacaga atgcgtgtgc
    28921 gtaaaccttg ctggcattta aaatgaatgc tcaacagtaa agtgaacatg acgatcactt
    28981 tagtaaacgg taccagaata ctggcgataa acacgattcc tgcaaccgca atgttgctac
    29041 ttgccagcga cataattccg gaaagaattg tatcttcctg ccgtcctccg ttcagataaa
    29101 tgatagaaat aggcaacagg ttggcaggta acaataaaac gattgacgct aacagcgccg
    29161 cccagcattt ttgcagacta tgacggcgac gcaggcgtag cggaatatga caacgcgggc
    29221 agcgaccacg ctgatcagga tagccggtaa aatggcaccc aagacagaca cgaagtttct
    29281 cgtccctacg cgtagcgggg cgctgcggat aaaatcgctc ccacagctcc tcgacattaa
    29341 gatgtgacaa cgtcaccgtc gttaaaatca ccaacgccac aaaagagaac aagccgacac
    29401 ccgcctggat atgggcataa tcctgtacct ttatagaagc aacgccaatg ccgaccaggt
    29461 agatatcgag cattacccac tctttaagtc gctcaagcat cagcagtacc gggcgtagat
    29521 tcattcccag tcggttacca aaccataaat aagctatgga ggtcaccaga atgagcgggg
    29581 caccaataac gcagaaaaag accatcgacc ccgttatcgc atcgccctgt ttggtcattt
    29641 gccagatgcc ttgcataacg ttggcgtcaa tacgaatgcc taacagccag atatgcaaca
    29701 gcggttcgcc ccaggcaaac ggcatcagca acagcatggt gaaggccatt gccgccaggc
    29761 gcgttagcga ccagtcgcgc ccgtcacgaa tttttgcctg acagcgcgga caataggcac
    29821 tttgatgaga atttatctcg ggcaggctaa acagcatgtc acattgcggg caacgttggt
    29881 aatcaccacg cggcagttcc tcgccgattg ccctcactgt tatctttttt gtcggcgtaa
    29941 tttgtggtgt gttaagagcc atgacgtgcc aaatgtaata agaattaaca ttaatcttaa
    30001 cccatgatgg gaatgatctt gagcattaac gcatttaatg cttattttaa taggctggca
    30061 gttccaggta agacaaccaa gtgattatat aatgaacaaa acagaatttt acgcggattt
    30121 aaatcgcgac tttaacgcgc tgatggcggg agaaaccagt tttctggcaa cgcttgcgaa
    30181 caccagtgcg ttgttatatg agcgtctcac tgacgtaaac tgggcaggtt tttatttgct
    30241 tgaggacgat acactggtgc tcggaccatt tcagggcaaa attgcctgtg tccggatacc
    30301 cgtcgggcgt ggcgtgtgcg gcactgcggt tgcccgcaat caagtgcagc gtatcgagga
    30361 tgttcatgcg tttgacgggc atattgcctg tgatgcggcg agtaattctg aaattgttct
    30421 gccgctggtg gtgaaaaatc agattattgg tgttctcgac atcgatagta ccgtcttcgg
    30481 tcgctttaca gacgaggacg agcaaggctt acgtcagctt gtggcacagc ttgaaaaagt
    30541 gcttgcaacg acggattaca aaaaattctt tgcgagcgtc gcaggataat caacggataa
    30601 cgtagcattt actgatagcg tcattataat gacgcctgtt catgcctgcg cttgttggct
    30661 acgtccgttg taatcaggaa atttcatgga aaatcaacct aagttgaata gcagtaaaga
    30721 agtaatcgcg tttctggccg aacgttttcc ccactgtttc agtgcggaag gtgaagcgcg
    30781 tccgctgaaa atcggtattt ttcaggattt ggtcgatcgt gttgccgggg aaatgaacct
    30841 gagcaaaacg caattacgat ccgctttacg tctctacact tcgagctggc gttaccttta
    30901 cggtgttaaa cccggcgcaa cgcgtgtcga tcttgacggc aacccatgcg gtgagctgga
    30961 cgagcaacat gtagagcatg ctcgcaagca gcttgaagaa gcgaaagcgc gtgttcaggc
    31021 acagcgtgct gaacagcaag cgaaaaaacg cgaagctgcc gcagctgctg gtgagaaaga
    31081 agacgcaccg cgtcgcgaac gcaagccacg tccgaccacg ccacgccgca aagaaggcgc
    31141 tgaacgtaaa cctcgtgcgc aaaagccggt agagaaagcg ccaaaaacag caaaagcacc
    31201 tcgcgaagaa cagcacaccc cagtttctga catttcagct ctgactgtcg gacaagccct
    31261 gaaggtgaaa gcgggtcaaa acgcgatgga tgccaccgta ttagaaatca ccaaagacgg
    31321 cgtccgcgtc cagctgaatt cgggtatgtc tttgattgtg cgcgcagaac acctggtgtt
    31381 ctgaaacgga ggccgggcca ggcatgaaca tgttttttag gcttaccgcg ttagctggcc
    31441 tgcttgcaat agcaggccag accttcgctg tagaagatat cacgcgtgct gatcaaattc
    31501 cggtattaaa ggaagagacg cagcatgcga cggtgagtga gcgcgtaacg tcgcgcttca
    31561 cccgttctca ttatcgccag ttcgacctcg atcaggcatt ttcggccaaa atctttgacc
    31621 gctacctgaa tctgctcgat tacagccaca acgtgctgct ggcaagcgat gttgaacagt
    31681 tcgcgaaaaa gaaaaccgag ttaggcgatg aactgcgttc aggcaaactc gacgttttct
    31741 acgatctcta caatctggcg caaaagcgtc gttttgaacg ttaccaatac gctttgtcgg
    31801 tactggaaaa gccgatggat ttcaccggca acgacactta taaccttgac cgcagcaaag
    31861 cgccctggcc gaaaaacgag gctgagttga acgcgctgtg ggacagtaaa gtcaaattcg
    31921 acgagttaag cctgaagctg gcaggaaaaa cggataaaga aattcgtgaa accctgactc
    31981 gccgctacaa atttgccatt cgtcgtctgg cgcaaaccaa cagcgaagat gttttctcgc
    32041 tggcaatgac ggcgtttgcg cgtgaaatcg acccgcatac caactatctt tccccgcgta
    32101 ataccgaaca gttcaacact gaaatgagtt tgtcgctgga aggtattggc gcagtgctgc
    32161 aaatggatga tgactacacc gttatcaatt cgatggtggc aggtggtccg gcagcgaaga
    32221 gtaaagctat cagcgttggt gacaaaattg tcggtgttgg tcaaacaggc aagccgatgg
    32281 ttgacgtgat tggctggcgt cttgatgatg tggttgcctt aattaaaggg ccgaagggca
    32341 gtaaagttcg tctggaaatt ttacctgctg gtaaagggac caagacccgc actgtaacat
    32401 tgacccgtga acgtattcgt ctcgaagacc gcgcggttaa aatgtcggtg aagaccgtcg
    32461 gtaaagagaa agtcggcgtg ctggatattc ctggcttcta tgtgggtttg acagacgatg
    32521 tcaaagtgca actgcagaaa ctggaaaaac agaatgtcag cagtgtgatc atcgatctgc
    32581 gtagcaatgg tggtggggcg ctgaccgaag cggtatcgct ctcaggtctg tttattcctg
    32641 cgggtcctat tgttcaggtc cgtgataaca acggtaaagt tcgtgaagac agcgataccg
    32701 acggacaggt tttctataaa ggcccgctgg tggtactggt tgaccgcttc agtgcttcgg
    32761 cttcagaaat ctttgccgcg gcaatgcagg attacggtcg tgcactggtt gtgggtgaac
    32821 cgacgtttgg taaaggcacc gttcagcaat accgttcatt gaaccgtatt tacgatcaga
    32881 tgttacgtcc tgaatggcca gcgctgggtt ctgtgcagta cacgatccag aaattctatc
    32941 gcgttaacgg cggcagtacg caacgtaaag gcgtaacgcc agacatcatc atgccgacgg
    33001 gtaatgaaga aacggaaacg ggtgagaaat tcgaagataa cgcgctgccg tgggatagca
    33061 ttgatgccgc gacttatgtg aaatcaggcg atttaacggc ctttgaaccg gagctactga
    33121 aggaacataa tgcgcgtatc gcgaaagatc ctgagttcca gaacatcatg aaggatatcg
    33181 cgcgcttcaa cgctatgaag gacaagcgca atatcgtttc tctgaattac gctgtgcgtg
    33241 agaaagagaa taatgaagat gatgcgacgc gtttggcgcg tttgaacgaa cgctttaaac
    33301 gcgaaggtaa accggagttg aagaaactgg atgatctacc gaaagattac caggagccgg
    33361 atccttatct ggatgagacg gtgaatatcg cactcgatct ggcgaagctt gaaaaagcca
    33421 gacccgcgga acaacccgca cccgtcaagt aatatcaatc aggcacaaga aattgtgcct
    33481 gattttttaa cagcggcaag atgccgtaaa tcagatgcta caaaatgtaa agttgtgtct
    33541 ttctggtgac ttacgcacta tccagacttg aaaatagtcg cgtaacccat acgatgtggg
    33601 tatcgcatat tgcgttttgt taaactgagg taaaaagaaa attatgatgc gaatcgcgct
    33661 cttcctgcta acgaacctgg ccgtaatggt cgttttcggg ctggtactga gcctgacagg
    33721 gatacagtcg agcagcgttc aggggctgat gatcatggcc ttgctgttcg gttttggtgg
    33781 ttccttcgtt tcgcttctga tgtccaaatg gatggcatta cgatctgttg gcggggaagt
    33841 gatcgagcaa ccgcgtaacg aaagggaacg ttggctggtc aatactgtag caacccaggc
    33901 tcgtcaggcg gggatcgcta tgccgcaagt ggctatctac catgcgccgg acatcaacgc
    33961 ttttgcaacc ggtgcgcgcc gtgatgcctc tctggttgct gtcagcaccg gtttgctgca
    34021 gaacatgagc ccggatgaag ccgaggcggt aattgctcac gaaatcagcc acatcgccaa
    34081 tggtgatatg gtcaccatga cgctgattca gggcgtggtg aacaccttcg ttatctttat
    34141 ttcccgtatc ctggcgcagc ttgccgcggg ttttatgggc ggaaatcgtg atgaaggtga
    34201 agagagcaac ggcaacccgc tgatctactt tgcggttgca acggttctgg aactggtgtt
    34261 tggtattctg gcgagcatta tcaccatgtg gttctcgcgt catcgtgaat tccatgctga
    34321 tgccggttcg gcaaaactgg ttggtcgcga gaaaatgatt gccgcgctgc agcgcctgaa
    34381 aaccagctat gaaccgcaag aagcaaccag catgatggct ttctgcatta acggtaagtc
    34441 gaaatcgctc agtgagttgt ttatgactca cccgccgctg gataaacgta ttgaagctct
    34501 gcgtacgggt gaatacctga agtaatatga agtaaatact aaaaagcgcg tcctgctcga
    34561 cgcgcttttt ttatgccctg gatcttggct gagtgatacg taacccactg atacaggcag
    34621 caatcactgc cagaatagcc gcagccatca gcgagacgtg tgtaccatta tctccaaact
    34681 gatttagcat cagcgccacc agcgccgcgc cgctactctg acccagtaga cgagccgttc
    34741 ctaacatgcc actggctcca ccgctacgtt cgcgaggcgc ggaggtaata atggtgtggt
    34801 tattgggtga ctggaacaag ccaaatccag cgccacataa gatcatcggc cagataatat
    34861 tgatatccgc aggtgacgcg ggcagcagaa ccagggaaaa aagccccgca gccatgatga
    34921 ataatcctaa agcacccagc aatcctgcat gtacgcgttc aatcaaatag cctgccaacg
    34981 gagccatcac catcgttgct aacggccacg gtgtcagaag taaacctgtt tcgacttcac
    35041 tacgcccgag cacggtttgc aggtaaaagg gcagggaaac cattgccagc atttgtgcgc
    35101 agaaagagca aacagatgtg caaatagaaa gtgaaaacag cgggatacgc agtaaatcca
    35161 ccggtagcag cggtactgga agagaaagct ggcggcgaat aaagaaaata ccaacgacaa
    35221 ccattacaac cagttccgca gcaattaacg tcagcgattg cccctgagcg aaaccactca
    35281 acgcagtgat aagcaggccg aaggttaacg cgttcatcac ggcgctgggc aggtcgaaac
    35341 ggggtttact ggcgcgagaa ccattgggtg gcagaaaacg catcgccaga agcagggcga
    35401 taatacccaa cggtacgttg attaaaaata accatttcca ggatgcgatg gagaggattg
    35461 ctgcagcaat tgtcggcccg gcagcagaag agacggcaac aataaacgag tttatgccca
    35521 tccctctacc cagaaaacgt tgtggataga tcaggcggat aagggcggta ttaacgctca
    35581 tcaacgctgc accgccgaaa ccttgtatga cacgcgcaag ggtgagtatt tgcagcgaat
    35641 cagaaagggc gcagaacagt gaagacaaca gaaaaacgac cagaccgcat ttataaatac
    35701 gtcgatagcc aaacatatca cccagaaacg aaaacgagag cagggagatg acaatggcga
    35761 tttgataggc gttcactacc cagatggaac tggctggcgt ggcatgaagg tccgtggcga
    35821 ttgttggcag ggcgacgttg gcgattgcgc cgtcaaggac agccatcgaa ataccaatca
    35881 caatggttaa tatcgcaccg tatcgctggg gcaatggcag gccgtcggcc tgaacttttg
    35941 gcataggaat tttaaatctt tggtgaataa tcaacagggg tatgattcta gcactgatat
    36001 tcacggtgga tgtcgcagaa ttgtaactaa ccggcaaaag atggattgcg accaccctgg
    36061 tgcgaaatta taataaaaaa cagttctgat ttttataaaa cactcgcaat gaggtgataa
    36121 atggctaacg cagatctgga taaacagcct gattctgtat cttccgtgct aaaagttttt
    36181 ggcattttgc aggcgctggg tgaagagcgc gaaataggga taaccgagct gtcgcagcgc
    36241 gtcatgatgt caaaaagcac cgtttatcgc tttttacaga ccatgaaaac cttaggttat
    36301 gtggcgcagg aaggggagtc ggagaaatat tccctgaccc tgaaattgtt tgaactgggc
    36361 gctcgcgcgt tacaaaacgt cgatttaatt cgtagcgcag atatccagat gcgtgagatc
    36421 tcccgcctga ccaaagaaac tatccacctc ggcgcactgg acgaagacag tattgtttac
    36481 attcacaaaa ttgactctat gtacaatttg cgcatgtatt cacggattgg gcgtcgtaat
    36541 ccactgtaca gcaccgcgat tggtaaggta ctgctggcat ggcgcgatcg cgatgaagtg
    36601 aagcaaattc ttgagggcgt ggagtataaa cgcagtaccg agcggaccat caccagtaca
    36661 gaagcgttat taccggttct ggaccaggtg cgcgagcagg ggtatggcga agataacgaa
    36721 gagcaggaag aagggctgcg atgcattgcg gttccggtat ttgatcgctt tggcgtggtc
    36781 attgccggtt tgagcatctc cttcccgacg ttgcgtttct ctgaagagcg tttacaggaa
    36841 tatgtcgcaa tgttgcatac cgcagcgcgc aaaatttctg cccaaatggg ttatcacgac
    36901 tatccgttct gatgagagta agaacctgtc ggaatatcaa acagacaggt tctttattta
    36961 gcatgagaaa aataaagttg aaggtggcgt tagattaaac gcagttgata taagaatatt
    37021 ttactcatga gtgagaatct ggttatttat tacacttaac cattatcgac cacgatattg
    37081 cttttgcgta acagcgggca atctgttatc cccaaaaaac cacttttagt gtgcaagtat
    37141 tgtaccgtgc tggtgcctct ggcagtcaga taggtacatt gcaaacctaa ttctgcggca
    37201 ttctctttgc ttccaatcaa aacgccatat ccgctgagta ataatcctat ccataccagt
    37261 gctatcagca taactgtgcg aatgatgaat cgcattacaa cctcttctct tttttatgtt
    37321 cgcttaatcg tagcggcaat atgcgctgaa gcaagcgact cattccgaaa aagcacgaat
    37381 atcgacatag ttagacgctg tttaactaac gcatggtagt ttaatgacat aaggtaggtg
    37441 aaacggagat tggagtgaaa aagtttcgat gggtcgtttt ggttgtcgtg gtgttggctt
    37501 gcttgctgct ttgggcgcag gtattcaaca tgatgtgcga tcaggatgta caatttttca
    37561 gcggaatttg tgccattaac cagtttatcc cgtggtgata tcattttttc agcactagat
    37621 tttcttcctc tccagcagtg gtagaatagc gcctctactc agaggtggtg aaatgaacga
    37681 agttgtaaat tcaggcgtga tgaacattgc gtctttggtt gtatcggtgg tggttcttct
    37741 tatcgggctc atcttgtggt tttttattaa tcgtgccagt tctcggacta acgaacagat
    37801 tgaactgctt gaggcgttgc tggatcagca aaaacgtcaa aatgcactgt tacgtcgttt
    37861 gtgcgaagca aacgaaccag agaaagcaga taaaaagact attgagagtc aaaaatcggt
    37921 tgaagacgaa gatattattc gcctggtcgc cgaacgataa tccttcctca atcttgagta
    37981 accccgtcgt tgaagggtta ctcaattcat cctgattata cgcactgtca tggtgcaact
    38041 gcttcaaatt aaa
//
LOCUS       DAZKIP010000032        37781 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZKIP010000032 DAZKIP010000000
VERSION     DAZKIP010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37781
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.032"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..804
                     /gene="ytfI"
                     /locus_tag="ACHOUK_001889"
     CDS             <1..804
                     /gene="ytfI"
                     /locus_tag="ACHOUK_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtfI"
                     /protein_id="HFP9897352.1"
                     /translation="NYDSLLTLREFFRCATQIDKSSYQVLSSKNETVNAMDKFLISFS
                     LKDNGAEYTMTLRGSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYAWDILQKKQ
                     SDIPKRIKGYIHQAIGDVSLGYALLDDIVSKLKRGKFELQGPGGGIKQCDGWYIYEKI
                     IDDNFAIVIESLGFALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQ
                     VDAIVSANLAADSDITKEKICEKYKSTIEAFKKEQLALPVLVRRKNSET"
     gene            complement(867..1421)
                     /gene="ytfJ"
                     /locus_tag="ACHOUK_001890"
     CDS             complement(867..1421)
                     /gene="ytfJ"
                     /locus_tag="ACHOUK_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFP9897353.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            1746..1952
                     /gene="ytfK"
                     /locus_tag="ACHOUK_001891"
     CDS             1746..1952
                     /gene="ytfK"
                     /locus_tag="ACHOUK_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFP9897354.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(2031..3374)
                     /gene="paeA"
                     /locus_tag="ACHOUK_001892"
     CDS             complement(2031..3374)
                     /gene="paeA"
                     /locus_tag="ACHOUK_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFP9897355.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(3697..4335)
                     /gene="msrA"
                     /locus_tag="ACHOUK_001893"
     CDS             complement(3697..4335)
                     /gene="msrA"
                     /locus_tag="ACHOUK_001893"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFP9897356.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLHGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            4541..6274
                     /gene="tamA"
                     /locus_tag="ACHOUK_001894"
     CDS             4541..6274
                     /gene="tamA"
                     /locus_tag="ACHOUK_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFP9897357.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            6271..10050
                     /gene="tamB"
                     /locus_tag="ACHOUK_001895"
     CDS             6271..10050
                     /gene="tamB"
                     /locus_tag="ACHOUK_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFP9897358.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGILNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQIYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGLEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELTVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTLVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTEEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            10053..10394
                     /gene="ytfP"
                     /locus_tag="ACHOUK_001896"
     CDS             10053..10394
                     /gene="ytfP"
                     /locus_tag="ACHOUK_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFP9897359.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            10606..10857
                     /gene="chpS"
                     /locus_tag="ACHOUK_001897"
     CDS             10606..10857
                     /gene="chpS"
                     /locus_tag="ACHOUK_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HFP9897360.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEVQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            10851..11201
                     /gene="chpB"
                     /locus_tag="ACHOUK_001898"
     CDS             10851..11201
                     /gene="chpB"
                     /locus_tag="ACHOUK_001898"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HFP9897361.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(11281..11811)
                     /gene="ppa"
                     /locus_tag="ACHOUK_001899"
     CDS             complement(11281..11811)
                     /gene="ppa"
                     /locus_tag="ACHOUK_001899"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFP9897362.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            12121..13077
                     /gene="ytfQ"
                     /locus_tag="ACHOUK_001900"
     CDS             12121..13077
                     /gene="ytfQ"
                     /locus_tag="ACHOUK_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFP9897363.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            13217..14719
                     /gene="ytfR"
                     /locus_tag="ACHOUK_001901"
     CDS             13217..14719
                     /gene="ytfR"
                     /locus_tag="ACHOUK_001901"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFP9897364.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFDLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETCELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQNLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            14733..15755
                     /gene="ytfT"
                     /locus_tag="ACHOUK_001902"
     CDS             14733..15755
                     /gene="ytfT"
                     /locus_tag="ACHOUK_001902"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFP9897365.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIIT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLILFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            15742..16737
                     /gene="yjfF"
                     /locus_tag="ACHOUK_001903"
     CDS             15742..16737
                     /gene="yjfF"
                     /locus_tag="ACHOUK_001903"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFP9897366.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPVYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(16770..17768)
                     /gene="fbp"
                     /locus_tag="ACHOUK_001904"
     CDS             complement(16770..17768)
                     /gene="fbp"
                     /locus_tag="ACHOUK_001904"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFP9897367.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYSTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFICEFPDA"
     gene            17944..19317
                     /gene="mpl"
                     /locus_tag="ACHOUK_001905"
     CDS             17944..19317
                     /gene="mpl"
                     /locus_tag="ACHOUK_001905"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFP9897368.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(19473..20024)
                     /gene="yjgA"
                     /locus_tag="ACHOUK_001906"
     CDS             complement(19473..20024)
                     /gene="yjgA"
                     /locus_tag="ACHOUK_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFP9897369.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            20118..21470
                     /gene="pmbA"
                     /locus_tag="ACHOUK_001907"
     CDS             20118..21470
                     /gene="pmbA"
                     /locus_tag="ACHOUK_001907"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFP9897370.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            21654..22040
                     /gene="cybC"
                     /locus_tag="ACHOUK_001908"
     CDS             21654..22040
                     /gene="cybC"
                     /locus_tag="ACHOUK_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFP9897371.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNSYHKKYR"
     gene            complement(22085..22549)
                     /gene="nrdG"
                     /locus_tag="ACHOUK_001909"
     CDS             complement(22085..22549)
                     /gene="nrdG"
                     /locus_tag="ACHOUK_001909"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFP9897372.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(22739..24877)
                     /gene="nrdD"
                     /locus_tag="ACHOUK_001910"
     CDS             complement(22739..24877)
                     /gene="nrdD"
                     /locus_tag="ACHOUK_001910"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFP9897373.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDISEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(25271..26926)
                     /gene="treC"
                     /locus_tag="ACHOUK_001911"
     CDS             complement(25271..26926)
                     /gene="treC"
                     /locus_tag="ACHOUK_001911"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFP9897374.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPDDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVALNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DESSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            complement(26976..28397)
                     /gene="treB"
                     /locus_tag="ACHOUK_001912"
     CDS             complement(26976..28397)
                     /gene="treB"
                     /locus_tag="ACHOUK_001912"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFP9897375.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(28516..29463)
                     /gene="treR"
                     /locus_tag="ACHOUK_001913"
     CDS             complement(28516..29463)
                     /gene="treR"
                     /locus_tag="ACHOUK_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFP9897376.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPANLS"
     gene            29648..29701
                     /gene="mgtL"
                     /locus_tag="ACHOUK_001914"
     CDS             29648..29701
                     /gene="mgtL"
                     /locus_tag="ACHOUK_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFP9897377.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            29842..32538
                     /gene="mgtA"
                     /locus_tag="ACHOUK_001915"
     CDS             29842..32538
                     /gene="mgtA"
                     /locus_tag="ACHOUK_001915"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFP9897378.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(32744..33130)
                     /gene="ridA"
                     /locus_tag="ACHOUK_001916"
     CDS             complement(32744..33130)
                     /gene="ridA"
                     /locus_tag="ACHOUK_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFP9897379.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(33203..33664)
                     /gene="pyrI"
                     /locus_tag="ACHOUK_001917"
     CDS             complement(33203..33664)
                     /gene="pyrI"
                     /locus_tag="ACHOUK_001917"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFP9897380.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(33677..34612)
                     /gene="pyrB"
                     /locus_tag="ACHOUK_001918"
     CDS             complement(33677..34612)
                     /gene="pyrB"
                     /locus_tag="ACHOUK_001918"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFP9897381.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(34616..34750)
                     /gene="pyrL"
                     /locus_tag="ACHOUK_001919"
     CDS             complement(34616..34750)
                     /gene="pyrL"
                     /locus_tag="ACHOUK_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFP9897382.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(35031..35426)
                     /gene="yjgH"
                     /locus_tag="ACHOUK_001920"
     CDS             complement(35031..35426)
                     /gene="yjgH"
                     /locus_tag="ACHOUK_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFP9897383.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPTWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(35557..36270)
                     /gene="bdcA"
                     /locus_tag="ACHOUK_001921"
     CDS             complement(35557..36270)
                     /gene="bdcA"
                     /locus_tag="ACHOUK_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9897384.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            36341..36934
                     /gene="bdcR"
                     /locus_tag="ACHOUK_001922"
     CDS             36341..36934
                     /gene="bdcR"
                     /locus_tag="ACHOUK_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9897385.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLVDILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARLHPQSAQCVTDFM
                     STVMSGLSAKAREGHSLEQLCATAALAGEAIKTILKE"
     gene            37079..37531
                     /gene="tabA"
                     /locus_tag="ACHOUK_001923"
     CDS             37079..37531
                     /gene="tabA"
                     /locus_tag="ACHOUK_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFP9897386.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            37654..>37781
                     /locus_tag="ACHOUK_001924"
     CDS             37654..>37781
                     /locus_tag="ACHOUK_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9897387.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNNFARNAGG"
BASE COUNT         9126 a         9369 c        10140 g         9146 t
ORIGIN      
        1 aattatgata gcctgttaac tttgagggag ttctttcgtt gcgcaactca gattgataaa
       61 agtagttatc aggtgctttc atcaaaaaat gaaactgtta acgctatgga taagtttctt
      121 atcagtttca gccttaaaga taacggtgcc gaatatacaa tgacattgcg aggcagtgga
      181 tttgaatacg aagaaatccc cataacaata aatgagtata actcattcat ggatttcaag
      241 aaccgtgaat ttccgttaga acaaaacaga cgattgtatg cctgggatat tctgcagaaa
      301 aaacagtctg acataccgaa aaggattaaa ggttatattc accaggcgat tggtgacgtg
      361 tctttaggtt atgctttatt ggatgatata gtaagcaaat taaaacgggg taaatttgaa
      421 ctacaagggc caggaggtgg aattaaacag tgcgatggat ggtatattta cgaaaaaata
      481 attgatgata attttgccat agtcatagaa tctttgggct ttgcgcttaa aatctatgga
      541 ggggatgaac gttttcgtaa tggttcctct gttgtcttgg aggatgaaga ttactctctt
      601 atttataatt ttctggttaa tgcgggttgc caacaagtcg aattagccga acaagtggat
      661 gcgatagtaa gtgcgaatct ggccgcagat agtgatatta ccaaagaaaa gatttgcgag
      721 aaatataaat cgaccattga ggcgtttaaa aaagagcaac tagcgttacc agtattagtt
      781 cggcgtaaga actcagagac ttaattcccc tatcccatag ataacgatag gggaaaatta
      841 tttttgcaga atttatgtta ttgccattat ttgagcaact tttgcagcag gtccatcact
      901 tgctgcacct cttcctgagt gagcgccccg tctttggccc attgcacgcg accgtcttta
      961 tccagcacca ctacagcgga actttcttca tccagctgcc aggcaccgcg cgcgacgcca
     1021 ttgctatcga caataaactg cgaccaggga taaagctttt tattactctc cagactgctg
     1081 cgcacaaaca tgccggaacc cggaatcgcg tcgtcggtgt taacaatggt ggtggtctgg
     1141 taacgatcgt gcggtaactt cgctgattta atcgcttcaa tcagcgtcgc gtttttctct
     1201 tttgcagagg tgcgaccagc aatatgttgc agtactcgca cttttcccac taactgcgcg
     1261 ctgttccagg ttttgtagct aaactgatct ttatcaagca ccaactcgcc ccgatcggta
     1321 atgccaatcg gcggcactcg ctgaccggtt tcgaactgat gtgcggaagc catcatcggc
     1381 aacagcaggc aggtgagtgc cagaatcttg cgtagggtca tggtgtttcc ttcttatgat
     1441 atgcaggtga tccgaccact tgggtctgag ttttaatcat atgtgctatt tatcgaattt
     1501 cccgcaagtg tgatgccagt ttgcggtcaa gcgcacaaat catatgaaaa atgaatgctt
     1561 atactgaaga ccgcgcttcg gtaaaaagat aattctgaat aattgtaacc tttaggtaaa
     1621 aaaagttata cgcggtggaa acattgcccg gatagtctat agtcactaag cattaaaatt
     1681 tgcgcctcat aatagttggg ccgattgtgg caccgcacag gcgtaatact cagcaggaga
     1741 taacaatgaa aattttccaa cggtacaacc cacttcaggt ggcgaagtac gtaaagatcc
     1801 tgttccgtgg acgattatac atcaaggacg ttggcgcttt tgaattcgat aagggtaaga
     1861 ttcttatccc aaaagtgaag gataagctgc atttgtcagt gatgtccgaa gttaaccgtc
     1921 aggttatgcg tctgcaaaca gagatggctt aaccaaagtg ctatgcagta aaaagtgcta
     1981 tgcagtaata agacggctcc tgattcagga gccgttgatg tttatggggg ttacgcgacg
     2041 ctttcttctt tatctttagc gtcaggcagt tttggcgaaa gggctgtggc cttgctgtcg
     2101 atccgggtca ccagcagctg gtcgatgcgg tagttatcga tatccaccac ttcaaatttg
     2161 tagccggcga atttcaccga atcggtgcgt ttcgggatct tacgcagcat aaacatcata
     2221 aagccgccga tggtttcgta gttgcccgac tgcgggaact cgtcaatatc cagcacgcgc
     2281 atgacgtcgt caatcggagt gccgccgtca atcagccatg agttctcatc acgggcgaca
     2341 atctgctctt ccagtccctg accgaccaga tcgcccatca gcgtggtcat cacgtcgttg
     2401 agggtgatga tccccactac cagcgcgtac tcgttcatga tcaccgcgaa gtcttctcct
     2461 gcggttttaa aactttccaa cgcctctgaa agggttaacg tatccggcac aatcagcgtg
     2521 ttgcgaattt gtacgccgct gttcagtgcc aggctttggt tagccagcac gcggttcagc
     2581 aggtctttag aatcgacata gccgatgatg tggtcaatat cttcattaca gacgaggaac
     2641 ttagagtgcg gatgttccgc caccttattt ttcagacttt gctcatcttc gtggagatca
     2701 aaccaaatca cgttttcacg cggtgtcatt gaagacggaa cggtacggga ttccagctca
     2761 aagacgtttt caatcagctc gtgttcctgt ttacgtaaca cgcccgccag cgcaccggct
     2821 tccactaccg cgtagatgtc atcagaagtg atgtcatctt tacgtaccat tggcagtttg
     2881 aaaatacgga agattatgtt cgccaggccg ttgaagaacc acaccagcgg ggtgcaaacg
     2941 tacaggcaga agcgcatcgg gttgatgata cgcaaagcca cagcttctgg cgcaatcata
     3001 ccgatgcgtt tcggggttaa atccgcaaac aggataaaca tgccagtcac taacgagaaa
     3061 gagagaataa agctcagttg ctcagagagc tctgccgaca tatagcggga gaacaggctg
     3121 tgaaaagctg gagaaaatgc cgcatcaccg acgataccgc cgagaatcgc cactgcgttc
     3181 agaccgattt ggaccacggt aaagaacatg ccgggatttt cctgcatatt cagaacgcgt
     3241 tgggcattta tattgccttc atcagccagc agtttaagtt tgattttgcg tgaggcggca
     3301 agtgagatct cagacatcga gaagaacgca cttacagcga tcaagcagag tatgactaaa
     3361 atactgttta acatatctta tccgaccttt caggccagat cctcggaagg ggaagttgat
     3421 taatttgtgt gaaatacaca ttgaaagccg attcgaagaa agtgaaccgg cattttcagt
     3481 ggctagtata gcgtaaggta ctgtaaagcc gccagagggt taaaattcag acagctgaaa
     3541 aatgcaaaac tgcctgatgc gctatgccga tcaggcctac gcagtccttg caatatattg
     3601 aatttggacg attttgtagg cctggcaagg cgtttacgcc gcatccggca taaacaaaac
     3661 gcactttatc aacaatctac atttgtaccc gtaacgctat gcttccggtg gcagacagac
     3721 gccaattccg ccaattccac agtaaccgta cgggttttta tgcagatatt gctggtggtc
     3781 atcttcggca taataaaacg gtgtggcgtt agcgatttcc gtggtgatgt gacgatcgtc
     3841 atcggcggca agcatcgccg cctgaaaacg ttccagactg gcgcgagctg cggcatcctg
     3901 ttctggggtc agcggataaa tcgctgaacg atactgcgtg ccgtggtcat tgccctgacg
     3961 cattccctgg gcgggatcgt gattctccca aaatacctgt agcaactgct catagctgat
     4021 gacggaagga tcgtaaacaa tgcgtaccgc ttcggcatga ccagtatcac cggagcacac
     4081 ttcccgataa gtcggatttg gcgtatagcc gccggtataa cctgcggcgg tgctgtaaac
     4141 gccgtgtaac tgccagaaca gacgctccac accccagaaa caacccatcg caaaaatggc
     4201 aatctccatt ccgtcaggta cattggtcat tgagtgaccg ttaaccgcat gcagcgtggc
     4261 tacgggcatc ggggtgttac gtccaggcag ggcatcggcg ggggaaacca gatgcttttt
     4321 atcaaataaa ctcatggtgt cgctctcccg aaaatcggtc attggggtta aggttgtaac
     4381 aagaggcgta tttgcacaca ataaccaccg tgaataggtc taaagtaaaa cataagaaat
     4441 atttggggtt tagtctgctt tttaatccat attactggat ttttgttaag ccgtttaacg
     4501 gcgttccagg ggcaggaaaa aaggatattc aggagaaaat gtgcgctata tccgacagtt
     4561 atgctgtgta agcttactct gcttaagcgg atctgccgtc gccgcgaacg tccgtctaca
     4621 ggtcgagggg ttatcgggac agctggaaaa gaacgttcgt gcgcagcttt ctacgattga
     4681 aagtgatgaa gtgacgccag accgtcgctt tcgcgcacgc gtcgatgatg ctatccgcga
     4741 aggtctgaaa gcgctgggtt attaccagcc gaccattgaa tttgatctcc gtccaccgcc
     4801 aaagaaaggg cggcaggtat tgatcgccaa agtcacgcca ggcgtgccgg tgttaattgg
     4861 cggcaccgat gtggtattgc gcgggggcgc gcggaccgat aaagactatt tgaaattgct
     4921 cgatactcgc ccggctattg gcacggtact gaaccagggc gattatgaaa atttcaaaaa
     4981 gtccttaacc agcattgcgt tgcgtaaagg ttatttcgat agcgaattta ccaaagcgca
     5041 gctgggcatt gcgctcggcc tgcataaagc cttctgggat attgattata acagtggcga
     5101 acgttaccgc tttgggcatg tgacctttga aggatcacaa atccgcgatg aatacctgca
     5161 aaatctggtg ccgtttaaag agggcgatga gtacgaatcg aaagatctgg cagaactgaa
     5221 ccgccgactt tctgctaccg gctggtttaa ctcggtggtg gtggctccac aatttgataa
     5281 agcgcgcgaa acgaaagtat taccattgac gggcgtggtt tcgccgcgaa cagaaaacac
     5341 tatcgaaacc ggggtcggtt actctacgga cgtgggaccg cgcgtgaaag cgacgtggaa
     5401 aaaaccgtgg atgaactcat acggtcacag tctgaccacc agtaccagta tttccgcgcc
     5461 ggaacagacc ctcgacttca gctataaaat gccgctgctg aagaatccac tggaacaata
     5521 ttatttggtg cagggcggtt ttaagcgcac tgacctgaac gataccgagt ctgactccac
     5581 tacgctggtg gcttctcgct actgggatct ctccagcggc tggcagcgtg ccattaacct
     5641 gcgctggagt ctcgaccact ttactcaggg tgaaattacc aacaccacga tgctgtttta
     5701 tcctggggtg atgattagcc gcacgcgttc tcgtggtggc ctgatgccaa cctggggcga
     5761 ctcgcaacgc tactctatcg actactccaa cacggcctgg ggttcagatg tcgatttctc
     5821 cgttttccag gcgcagaacg tctggatccg cacactgtac gatcgccatc gttttgtgac
     5881 acgcggcacg ctgggctgga ttgaaaccgg tgatttcgac aaagtaccgc cggatctgcg
     5941 tttcttcgcc gggggcgatc gcagtattcg cggctacaaa tacaaatcta tcgccccgaa
     6001 atacgctaac ggtgacctga aaggggcctc gaagttgata accggatcgc tggaatacca
     6061 gtacaacgtg accggaaaat ggtggggcgc ggtgtttgtc gatagtggcg aagcggtaag
     6121 cgatattcgc cgcagcgact ttaaaaccgg taccggggtc ggcgtacgct gggaatcgcc
     6181 ggtcgggcca atcaaactcg attttgccgt accggtcgcg gataaagacg aacacgggtt
     6241 acagttttac atcggtctgg ggccagaatt atgagtttat ggaaaaaaat cagcctcggc
     6301 gtggttatcg ttatcttact gttgctggga tcggtggcgt ttctggtggg caccaccagc
     6361 ggcctgcatc tggtatttaa agcggcggat cgctgggtgc caggactgga tattggcaag
     6421 gtcaccggcg gctggcgcga tctcaccttg tctgacgttc gttatgagca gccaggcgtg
     6481 gcggtaaaag cgggtaatct gcatctggcg gtcggtcttg agtgcctgtg gaacagcagt
     6541 gtttgtatta atgacttggc gctgaaagac attcaggtca acatcgacag taaaaaaatg
     6601 cctccttctg aacaggttga agaagaggaa gatagcggtc cgctggatct ctccacgccg
     6661 tatcccatca ccctgacacg ggtggcactg gacaacgtca acatcaagat tgatgacacc
     6721 acggtatcgg tgatggactt cacctccggc ctgaactggc aggagaaaac cctgaccctg
     6781 aaaccgacgt cgctgaaagg cctgctgatt gctctgccga aagtggcgga agtggcgcag
     6841 gaagaagtgg tcgaaccgaa aattgaaaat ccgcagccgg aggaaaagcc tctcggcgaa
     6901 acactcaaag atctcttttc tcgcccggta ttgccggaaa tgaccgacgt gcatctgcca
     6961 ctcaacctga acattgaaga gtttaaggga gagcaactgc gcgtgacggg cgatacggac
     7021 attaccgtgc gcaccatgct gctgaaagtg agcagcattg acggcaatac taaactggac
     7081 gccctggata tcgattccaa tcaagggatc ctcaacgcca gcggcacggc gcagctgtca
     7141 gacaactggc cggtggatat caccctcaac agcacactga acgtggagcc gttgaaaggt
     7201 gaaaaggtga agctgaaagt gggcggcgcg ctgcgcgaac agctggagat tggcgttaat
     7261 ctttccggtc cggtggatat ggatttacgc gcccagacgc gactggcgga agccggattg
     7321 ccgctcaacg tggaagtgaa cagcaaacag atttactggc cgttcactgg tgagaagcag
     7381 tatcaggcgg atgatctgaa actgaaactt accggcaaaa tgaccgatta cacgctctct
     7441 atgcgtacgg cagtgaaggg actggagatc ccgccagcca ccattaccct tgatgccaaa
     7501 ggtaatgaac agcaggtcaa tctcgacaaa ctcaccgtcg cggcgctgga agggaaaact
     7561 gaactcaagg cgttgcttga ctggcagcag gccattagtt ggcgcggtga gctaacgctt
     7621 aacggcatta acaccgccaa agagatccca gagtggccgt cgaaactcaa tggcttgatt
     7681 aaaacccgcg gtagcctgta cggcggcacc tggcagatgg aggtgccaga actgaagctt
     7741 accggtaacg tcaaacagaa caaagtgaac gttgacggca cgctgaaagg caacagttat
     7801 atgcagtgga tgatcccagg gcttcatctg gaactggggc caaacagtgc cgaagtgaaa
     7861 ggcgagctgg gggtaaaaga tctcaatctt gatgccacca tcaacgcgcc ggggctggat
     7921 aacgcgctgc cggggcttgg cggtacagcg aaagggctgg tgaaagttcg cggcacggtg
     7981 gaagcgccac aactactggc ggacatcacc gcgcgcggcc tgcgctggca ggagctaacc
     8041 gtggcgcagg ttcgcgtgga aggcgatatc aaatccaccg atcagattgc gggtaaactc
     8101 gacgtgcgcg ttgagcaaat ttcacagccg gatgtaaata tcaacctcgt caccctgaat
     8161 gccaaaggca gcgaaaaaca gcacgagtta cagttgcgga ttcagggcga gccggtttcc
     8221 gggcagctta atctggcagg aagttttgat cgcaaagaag aacgctggaa gggaactctt
     8281 agcaataccc gcttccagac gctggtcggc ccgtggtcgc tgacccgcga tattgcgctg
     8341 gattaccgca ataaggagca aaaaatcagc atcgggccac actgctggct taacccgaat
     8401 gcggaactgt gcgtgccgca aactatcgat gcgggggccg aagggcgtgc ggtggtgaat
     8461 ctcaaccgct tcgaccttgc catgctgaaa ccgtttatgc cagaaaccac tcaggccagc
     8521 ggtatcttca cgggtaaagc ggacgttgcc tgggacacca ccgaagaggg gctgccgcag
     8581 ggcagtatca ccctttcggg gcgtaacgtg caggtaacgc aaaccgtcaa cgatgcggcg
     8641 ctgcccgtgg cgtttcagac actgaatctg acggcggaat tgcgtaacaa ccgtgccgaa
     8701 ttgggctgga ccatccgcct gaccaataac ggccagtttg atggacaggt gcaggtgacc
     8761 gatccgcaag gccgccgtaa tcttggtggc aacgtcaata tccgtaactt caaccttgcg
     8821 atgataaacc ccatctttac ccgtggggaa aaagcagcgg ggatggtgag tgccaacttg
     8881 cgtctgggtg gtgatgtgca aagcccgcag ttgtttggtc agcttcaggt tacgggtgtg
     8941 gatatcgatg gcaactttat gccgtttgat atgcaaccga gccagcttgc ggtcaacttt
     9001 aacggtatgc gctcgacgct tgccggtaca gtacgaaccc agcagggtga aatctacctg
     9061 aacggtgatg ccgactggag ccaaattgaa aactggcggg cgcgggtaac ggcgaaaggc
     9121 agtaaagtgc ggatcaccgt gccgccgatg gtacgaatgg atgtatcgcc agatgttgta
     9181 ttcgaggcta caccaaacct gtttaccctc gatggtcgcg tggatgtccc ttgggcgcgc
     9241 atcgtggtgc acgatctgcc ggaaagcgca gtaggcgtct ccagcgatgt ggtgatgctt
     9301 aacgataacc tgcaaccgga agagccgaaa acggcgtcga ttccgattaa cagtaacctg
     9361 attgtccacg ttggcaacaa tgtgcgcatt gacgcctttg gcctgaaagc gcggctgacg
     9421 ggcgatctta acgtcgttca ggacaaacaa gggctgggcc tgaacgggca gatcaacatc
     9481 cctgaagggc gcttccatgc ctatggtcag gatctgattg tgcgtaaagg tgagttactg
     9541 ttctctggcc cgccggatca accgtatctt aatattgaag ctattcgtaa cccggatgct
     9601 acagaagacg acgtaatcgc cggagttcgc gtcactggtc tggcggacga accgaaagcg
     9661 gagatcttct ctgacccggc gatgtcgcaa caagctgcct tgtcttattt gctacgtgga
     9721 caagggctgg agagcgatca gagcgacagt gcggcaatga cctcgatgct gattggtctg
     9781 ggggttgcgc aaagtggcca gattgtaggt aaaatcggcg agacgtttgg cgtaagcaat
     9841 ttagcgctcg acacccaggg agtaggcgac tcctcccagg tagtggtcag cggctatgta
     9901 ttgccaggtc tgcaagtgaa atacggcgtg ggtatatttg actctatagc aacactcacg
     9961 ttacgttatc gcctgatgcc taagctatat ctggaagccg tgtctggtgt agaccaggca
    10021 ctggatttgc tctatcagtt cgagttttag caatgcgaat atttgtctac ggcagtttac
    10081 gccacaaaca aggcaacagt cactggatga ccaatgccca gttactgggc gatttcagta
    10141 tcgataacta ccagttgtat agcctgggcc actatccagg cgcagttccg gggaacggaa
    10201 cggtacacgg tgaagtttat cgtattgaca acgccacgct ggccgaactt gatgccttgc
    10261 gcaccagggg cggtgaatac gcgcgccagt tgattcagac gccgtacggg agtgcatgga
    10321 tgtacgttta tcaacgaccc gtcgatggat taaagctaat tgaaagcggc gactggttag
    10381 acagggataa gtaaccctat gcatacgcca ccttcgggtg gcgttgtttt ttgcgagacg
    10441 actcgcattc tgttttgtaa ttccctcacc ttttgctttt ctctccgagc cgctttccat
    10501 atctattaac gcataaaaaa ctctgctggc attcacaaat gcgcaggggt aaaacgtttc
    10561 ctgtagcacc gtgagttata ctttgtataa cttaaggagg tgcagatgcg tattaccata
    10621 aaaagatggg ggaacagtgc aggtatggtc attcccaata tcgtaatgaa agaacttaac
    10681 ttacagccgg ggcagagcgt ggaagtgcag gtgagcaaca accaactgat tctgacaccc
    10741 atctccaggc gctactcgct tgatgaactg ctggcacagt gtgacatgaa cgccgcggaa
    10801 cttagcgagc aggatgtctg gggtaaatcc acccctgcgg gtgacgaaat atggtaaaga
    10861 aaagtgaatt tgaacgggga gacattgtgc tggttggctt tgatccagca agcggccatg
    10921 aacagcaagg tgctggtcga cctgcgcttg tgctctccgt tcaagccttt aatcaactgg
    10981 gaatgacgct ggtggccccc attacgcagg gtggaaattt tgcccgttat gccggattta
    11041 gcgttccttt acattgcgaa gaaggcgatg tgcacggcgt ggtgctggtt aatcaggtgc
    11101 ggatgatgga tctacgcgcc cggctggcaa agcgtattgg tctggctgcg gatgaggtgg
    11161 tggaagaggc gttattacgc ttgcaggcgg tggtggaata aggtgtgttt atttatcgcg
    11221 ggcataaaaa aacccttact aaccgaagcc cggcgttcag ggttattacg ccagaagaac
    11281 ttatttattc tttgcgcgct cgaaggaggc aacgatttca gctttagcgg cttctgcgtt
    11341 ttcccaacct tcaactttca cccacttgcc tttttcgagg tctttgtagt gctcgaagaa
    11401 gtgagcgatt tgcgctttca gcagttcagg cagatcgtta acgtctttaa tgtgatcgta
    11461 ttctttgctc agcttgctgt gcggaaccgc aaccagtttc gcatcttcac cggcttcgtc
    11521 ggtcattttc agaacgccaa ccggacggca acggatcaca gaacccggct gcagcgggta
    11581 cggagttggg accagtacgt caactgggtc accgtccaga gacagggtgt ggttgatgta
    11641 accgtagttg cacggataga acatcgcggt ggacatgaag cggtcaacga acagtgcgcc
    11701 gctctctttg tcgatttcgt atttgatcgg atctgcgtta gccgggatct caataacaac
    11761 gtagatgtct tccggcagat ctttacccgc agggacgttg agtaagctca tgtctgtttc
    11821 ctttaaaaat atgtggcaaa caagtgccga gtattatagc caactcgcgc cgaatgtctt
    11881 cgcttgtttt cgtcttcatt ttcctttttc gccagtttta agacggtatg cagagcagga
    11941 aaatccataa ccgtagccgc atttttcata gtgagatgaa agcgattaca aacttgtgat
    12001 taacgtttta tttacttttt tgaagtgtga tgtaacgcaa tccgttacat aacgaattgt
    12061 ctatagtttt ttcgcgaaca tcttttcacc aataataatt actccgacga ggacaaccct
    12121 atgtggaaac gcttacttgt agtctctgca gtctcggcag ccatgtcgtc tatggcgttg
    12181 gccgctccat taaccgtagg attttcgcag gtcggatcgg aatccggctg gcgcgccgca
    12241 gaaaccaatg tggcgaaaag tgaggccgaa aaacgcggaa ttacgctgaa aattgccgat
    12301 ggtcagcaaa agcaggaaaa ccagattaaa gcggtacgtt ccttcgttgc acaaggggtg
    12361 gatgcgatct ttatcgctcc ggtggtcgcg acaggttggg aaccggtatt aaaagaggcg
    12421 aaagatgccg aaatcccggt cttcttgctc gaccgttcca tcgatgtgaa agacaaatct
    12481 ctctatatga ccaccgttac tgccgacaac atcctcgaag gtaagttgat cggtgactgg
    12541 ctggtaaaag aagtgaatgg caaaccatgc aacgtggtgg agctgcaggg caccgttggg
    12601 gccagcgtcg ccattgaccg taagaaaggc tttgccgaag ccattaagaa tgcgccaaat
    12661 atcaaaatca tccgctcgca gtcaggtgac ttcacccgca gtaaaggcaa agaagtcatg
    12721 gagagcttta tcaaagcgga aaacaacggc aaaaacatct gcatggttta cgcccataac
    12781 gacgacatgg tgattggtgc aattcaggca attaaagaag cgggcctgaa accgggcaaa
    12841 gatatcctca cgggttccat tgacggtgta ccggacatct acaaagcgat gatggatggc
    12901 gaagcgaacg ccagtgttga actgacgccg aacatggcgg gacccgcttt cgatgcgctg
    12961 gagaaataca aaaaagacgg caccatgcct gaaaagctga cgttaaccaa atccaccctt
    13021 tacctgcctg ataccgcaaa agaagagtta gagaagaaga aaaatatggg gtattgaggg
    13081 ttgctatgcc tgatgccgat tcgtaggccg gataaggcgc tcgcgccgca tccggcgatg
    13141 gtgcactgaa gcctgatgcg acgcttaccg cgtcttatca tgcctactgg gagcacgctt
    13201 tacaccgggg gaaaccatga cgaccgacca acaccaggag atcctccgca ccgaaggatt
    13261 aagtaaattt ttccccggcg tcaaagcgtt agacaacgtt gatttcagcc tgcgccgtgg
    13321 cgaaatcatg gcgctgctcg gtgaaaacgg ggcgggaaaa tcaacgctaa tcaaagcatt
    13381 aactggtgta taccacgctg atcgcggcac catctggctg gaaggccagg ctatctcacc
    13441 gaaaaatacc gcccacgcgc aacaactcgg cattggcacc gtctatcagg aagtcaacct
    13501 gctacccaat atgtcggtcg ctgataatct atttataggc cgcgaaccca aacgcttcgg
    13561 ccttctacgc cgcaaagaga tggaaaagcg cgccaccgaa ctgatggcat cttacggttt
    13621 ctccctcgac gtgcgcgaac cgctcaaccg cttttcagtc gcgatgcagc aaatcgtcgc
    13681 tatttgccgg gctatcgatc tctctgccaa agtgctgatc ctcgatgaac ccaccgccag
    13741 tctcgacacc caggaagtgg agttactgtt tgacctgatg cgtcagttgc gcgatcgcgg
    13801 cgtcagcctg atctttgtca ctcactttct cgatcaggtc tatcaggtca gcgatcggat
    13861 caccgtctta cgcaacggca gtttcgtagg ctgtcgggaa acgtgcgagc taccgcagat
    13921 cgaactggta aaaatgatgc tggggcgcga gctggatacc cacgcgctac agcgtgccgg
    13981 gcgaacattg ttgagcgaca aacccgttgc cgcgttcaaa aattacggca aaaaaggaac
    14041 gatcgcaccg tttgatctcg aagtacgccc cggcgagatc gtcggtctgg ctggattgct
    14101 gggatcagga cgtaccgaaa ccgccgaagt gatcttcggt atcaaacctg ctgacagcgg
    14161 cacggcgttg atcaaaggca aaccgcaaaa cctgcgatcg ccacatcagg cttcggtact
    14221 tggcattggc ttctgcccgg aagacaggaa aaccgatggc atcatcgctg ccgcctcggt
    14281 gcgggaaaat atcatcctcg ctctccaggc ccagcgcggc tggctacgtc ccatttcccg
    14341 caaagaacag caagagattg ccgaacgctt tatccgccag cttggcattc gcacaccttc
    14401 aactgaacaa ccgattgaat ttctctccgg cggcaatcag caaaaagtgt tgctttcacg
    14461 ttggctactg acccgaccgc aatttctgat cctcgatgag ccaacccgcg gcattgatgt
    14521 tggtgcccac gccgagatca tccgcctgat tgaaacgcta tgcgccgatg gtctggcgct
    14581 gctggtgatc tcctccgaac tggaagaact ggtgggctat gccgaccggg tgatcatcat
    14641 gcgcgatcgc aaacaggtgg cggagatccc gctggcagag ctttccgttc cggcgatcat
    14701 gaatgccatt gcggcgtaag gagaacagtg tgatgcctca atctctcccg gacactacgc
    14761 cgccaaaaag gcgctttcgc tggccaacgg gaatgccgca gctggcggca ctgttgctgg
    14821 tgctgctggt cgatagcctg gtggccccgc atttctggca ggtggtgctt caggatgggc
    14881 gtttgttcgg tagccctatc gacattctta atcgcgccgc ccccgttgcg ctactggcga
    14941 ttggtatgac gctggtgatc gcaacaggtg ggattgatct ctccgtaggg gcggtgatgg
    15001 ctatcgccgg agccacaacg gctgcgatga cggtcgcggg attcagcctg ccgattgttt
    15061 tgttaagcgc cctgggcacc ggcatcctgg cgggattgtg gaacggcata ctggtagcga
    15121 tcctcaaaat tcagccgttt gtcgccactc tgatcctgat ggtcgccggg cgcggcgtgg
    15181 cgcaactgat cacctccgga cagatcatca cgtttaactc gccggatctc tcatggttcg
    15241 gcagtggatc gctgttgttc ctgccaacgc cggtcattat cgcggtgctg acgcttatcc
    15301 tgttctggct gttgacccgc aaaacggcgc tggggatgtt tatcgaagcc gttggtatca
    15361 acattcgggc ggcaaaaaat gccggggtaa acacgcggat catcgtcatg cttacctacg
    15421 tgttgagcgg gctgtgtgcg gcgattgcgg gcattatcgt ggcggcggat attcgcggtg
    15481 ccgatgccaa caacgccgga ttatggctgg agctggacgc cattctcgcg gtggtgattg
    15541 gcggtggttc gctgatgggc ggacgtttta acctactgct ttcggtggtg ggggcgctga
    15601 ttattcaggg gatgaacacc ggaattttgc tttcgggctt tccgccagag atgaaccagg
    15661 tggtgaaagc ggtggtggtg ctttgcgtgc tgattgtcca gtcgcaacgc tttatcagtc
    15721 tgattaaagg agtgcgtagc cgtgataaaa cgtaatttgc cactgatgat caccatcggc
    15781 gtctttgtgc tgggttatct ctactgcctg acccagtttc ccggttttgc ttccacaaga
    15841 gtgatctgca atatcctgac cgataatgcc tttcttggga taatcgccgt tggcatgacc
    15901 tttgtgatcc tctccggtgg gatcgatctc tccgttggtt cggtgatcgc ctttaccggc
    15961 gtgtttctgg caaaagtgat tggcgatttc ggcctctcgc cgttgctggc atttccgctg
    16021 gtgctggtaa tgggctgtgc ctttggcgca tttatggggc ttttgatcga cgccctgaag
    16081 atcccggcat ttatcattac tttggcagga atgttctttt tgcgcggcgt cagctacctc
    16141 gtttcggaag agtcgatccc gataaaccat cctgtttatg acacgctctc aagccttgca
    16201 tggaaaattc ctggcggcgg tcgcttaagt gcgatggggc tgctgatgct ggcggtggtg
    16261 gttatcggca tattcctcgc gcatcgtacc cgctttggta atcaggtgta cgccattggc
    16321 ggcaatgcga cgtcggcaaa cctgatgggg atttccactc gcagcacaac tattcgcatt
    16381 tatatgctct ctaccggact ggcgacactg gcggggattg tcttctcgat ttatacccag
    16441 gccggatacg cgctggcggg cgtaggtgtg gaactggacg ctatcgcctc agtggtgatt
    16501 ggcggtacgc ttttgagcgg tggcgttgga acggtattag ggacgctttt tggcgtggcg
    16561 attcagggac tgattcagac ttacataaac tttgatggta cgctgagctc atggtggacg
    16621 aaaatcgcca tcggtatttt gttgtttatt tttatagcat tacagcgtgg attaacggtg
    16681 ctgtgggaga atcgtcagag ttcgcctgtg acaagagtca acattgcgca gcaataaaat
    16741 cgcctctccg tgtggagagg cgcaggagat tacgcgtccg ggaactcaca gataaagcgt
    16801 tcgacatctt caaccatatg gtcgttgccg acaaagaatg aacggcgctg gtgcagggtt
    16861 tccgggataa tatccagaat acgctctttg ccatcgctcg ctttaccgcc cgcttgttcc
    16921 gccaggaatg ccatcgggtt gcactcatac agcaaacgca gtttgccgtc cgggtggctg
    16981 gcggtgcttg ggtagagata aataccgcct ttcagtaggt tacggtggaa atccgcaacc
    17041 agtgagccga tataacgtga ggtatacggg cggttggtgg atttatcttc ttcctggcag
    17101 aatttaatgt acttcttcac cccgttcgga aacttaatgt agtttccttc gttgatggag
    17161 taggttttgc ctttctccgg gaagcgcatc cgttcctggc acaggcagaa aacgccgagc
    17221 gaaggatcgt aagtaaaagc gtgaacaccg catccggtac tgtaaaccag catggtagag
    17281 gagccgtata ccacgtaacc tgccgcaacc tgtttgttac caggctggag gaaatcttct
    17341 tccgttaccg gcgtgccgac gggcgtaact cggcggtaga tggagaaaat ggtaccgaca
    17401 gagacgttaa catcgatgtt ggacgagcca tccagtgggt ccatcagcac cacgtatttt
    17461 gcatgttcac agccttcaaa gacaacaatc tcatcttctt cttcagaggc aatgcctgca
    17521 acgatatcgc gtgctttcag tgcggctttc agtttttcat tagcgaacaa gtcgagtttt
    17581 tgctgaacct cgccctgcac gttctcagca ccgctggcac ccaggatatc aaccagtcct
    17641 gctttgttga tatcgcgatg gataatcttg gcgcccagtt ttattgccga cagcaaagca
    17701 gtgagctcac cggtagcatg agaaaactcg tgctgctttt cgacaataaa ttcacctaac
    17761 gttttcataa aactttccct gcaatgttta tggagtaaag cgaccgcaac aatcttaaca
    17821 aataatctca atgttgcgct caggtgaatc gcgccagcaa attacggatt atcctgaaat
    17881 gcgtttctca cttgcccgac atatgcgtaa aatgagcggc agattaaaaa aggatagtga
    17941 cgcatgcgca ttcatatttt aggaatttgt ggcacgttta tgggcggtct ggcgatgctg
    18001 gcgcgccagt taggccatga agtaacgggt tcggacgcca atgtgtatcc gccgatgagc
    18061 accttacttg agaagcaagg cattgagctg attcagggtt acgatgccag ccagctcgac
    18121 ccgcagccgg atctggtgat tattggcaac gccatgaccc gtggaaatcc gtgtgtggaa
    18181 gcggtactgg aaaaaaacat cccttatatg tcaggtccac agtggctgca cgattttgtg
    18241 ctgcgcgacc gctgggtgct ggccgttgcc ggtacacacg gcaaaaccac caccgcggga
    18301 atggcgactt ggattctgga acagtgtggt tacaaaccgg gctttgtaat cggcggtgtg
    18361 ccggggaact ttgaggtttc ggcgcgtctg ggcgaaagcg acttctttgt tatcgaagcg
    18421 gatgagtacg actgcgcctt cttcgacaaa cgctctaaat ttgtccatta ctgcccgcgt
    18481 acgctgatcc tcaacaacct tgagttcgat cacgccgata tctttgacga cctgaaagcg
    18541 atccagaaac agttccacca tctggtgcgt atcgttccgg ggcagggtcg tattatctgg
    18601 ccggaaaatg acatcaacct gaaacagacc atggcgatgg gctgctggag cgagcaggag
    18661 ctggtgggtg agcagggtca ctggcaggcg aaaaagctga ccaccgatgc ttccgaatgg
    18721 gaagttttgc tggatggcga aaaagtgggc gaagtgaaat ggtcgctggt aggcgaacat
    18781 aatatgcaca atggcctgat ggcgattgca gcggctcgcc atgttggtgt agcgccggca
    18841 gatgccgcta acgcgctggg ttcgtttatt aacgctcgcc gccgtctgga gttgcgtggt
    18901 gaagcgaatg gcgtcacggt atatgacgat tttgcccatc atccgacggc gattctggca
    18961 acgctggcgg cgctgcgtgg caaagttggt ggtacggcgc gcattattgc tgtgctggag
    19021 ccgcgctcga ataccatgaa aatggggatc tgcaaagacg atctggcacc ttcattaggt
    19081 cgtgccgatg aagtcttcct gctgcaaccg gcgcatattc cgtggcaggt ggcagaagtg
    19141 gcagaagcct gcgttcagcc tgcacactgg agtggcgatg tggatacgct ggcagatatg
    19201 gtggtgaaaa ccgctcagcc tggcgaccat attctggtga tgagcaacgg cggttttggt
    19261 gggatccatc agaaactact ggatggtctg gcgaagaagg cggaagctgc gcagtaattc
    19321 ggcctcagcc tgagatagtg tgcgtaggcc ggataaggcg ttcacgccgc atccggcatt
    19381 tgagcatagt gcctgatgcg acgcttgatg cgtcttatca ggcctacagg tgcaccgcat
    19441 ccggcaaacc atactcacat caacaacgaa aattaccctt cattctccgc cagttcgcgc
    19501 agatactgga aaatctggcg tgcggatttc ggcggcttat tcccttcttt ctctttcttc
    19561 gcgttgcgga tcagagtacg cagttgctga cgatcggcat cgggccacag atttaatacc
    19621 tcagcgatgg cgtcatcacc ttgatcgatc agacgatcgc gcaggttttc cagtttatgg
    19681 aacagcacca cctgctggtt gtgacggttt ttcagcttat ccagcgcctg acggatcggt
    19741 tctacatcgc gctggcgcag catcttaccg atgagctgca actggcggcg gcgaccttcc
    19801 atcttaatac gctgggcgag ctcaatagcc gcgcgcagat ccgcatctaa cgggatctta
    19861 tccagcgcgt ttttccccag atcaacaatt tccgcgccaa ggcgttttag ctcctcggca
    19921 tcacgtttaa tttcactttt actgacccag ataatttcat cgtcttcgtc ttcgatgtca
    19981 tcaccgggaa cgtcgtcgag ccagtcttcg ggctgcttag tcatctcagg ctccttaaaa
    20041 aaagaggcta atgttaccag ttaagatgcg cactgaaaaa cggttctctg ttagacttca
    20101 gagaaactct ctacattatg gcacttgcaa tgaaagtaat ctctcaagtt gaagcgcagc
    20161 gcaagattct ggaagaagca gtttccactg cgctggagtt ggcctcaggc aaatcggacg
    20221 gtgcggaagt tgccgtcagc aagaccaccg gcattagcgt aagcacgcgt tatggtgaag
    20281 tggagaatgt tgaattcaat agcgatggcg cgctggggat cactgtttat caccagaacc
    20341 gcaaaggtag cgcatcatcc accgatttaa gcccgcaggc cattgcccgt actgtacagg
    20401 cggcgctgga tattgcccgt tatacctcgc cagatccctg tgccggcgtg gcagacaaag
    20461 agctgctggc ctttgacgca ccggatctcg acttgttcca ccctgcggaa gtttccccgg
    20521 atgaagccat tgaactggcg gcccgcgcag aacaggcggc attgcaggcg gacaaacgca
    20581 tcaccaatac cgaaggtggc agctttaaca gccactacgg tgtcaaagtt tttggcaaca
    20641 gccacggcat gttgcagggt tactgctcaa cgcgtcattc gctctccagc tgtgtaattg
    20701 ccgaagaaaa tggcgatatg gagcgtgatt acgcctacac cattggccgc gcgatgagcg
    20761 atttgcagac gccagagtgg gtcggggccg attgcgctcg ccgtacttta tcgcgtctgt
    20821 caccgcgtaa actctccacc atgaaagcgc cggtcatttt tgccaatgaa gtggcaaccg
    20881 ggctttttgg gcatctggtg ggggcgatag cgggtggatc ggtttaccgt aaatctacct
    20941 tcctgctgga ttcgctgggt aaacaaattc tgccggactg gctgaccatt gaagagcatc
    21001 cgcatctgct gaaagggctg gcatcgacgc catttgacag cgaaggtgtg cgcaccgagc
    21061 gtcgcgatat tattaaagat ggcatcctga ctcagtggct gctgaccagc tactcggcgc
    21121 ggaaactggg gctgaaaagc accggacatg cgggcggtat tcacaactgg cggattgccg
    21181 gacaaggtct aagcttcgag cagatgctca aagagatggg caccgggctg gtggtgacgg
    21241 aattgatggg ccagggcgtg agtgcaatta ccggtgatta ttcccgtggt gcggcgggct
    21301 tctgggtaga aaacggcgaa attcagtatc cggttagcga aatcaccatc gcaggtaatt
    21361 taaaagatat gtggcgcaat attgtcaccg tcggtaacga tattgaaaca cgcagtaata
    21421 tacagtgtgg ttctgtgctg ttgccggaga tgaaaatcgc cggacagtaa aaaaactggc
    21481 gcgacctgcc gcgctataaa ctaaaaaatt acacaaaatc attcgcactg catcgagacg
    21541 gaaagtgaat gagggccggg gagcgtactc gcagtacgtg acccggtcga atgagcgtag
    21601 tcgtcgatga ggcagtgtga aggatgaagt gtaaataaaa aaggaagtga gcaatgcgaa
    21661 aaagcctgtt agctattctt gcagtctcct cgttggtatt cagttcggcg tcgtttgctg
    21721 ctgatcttga agacaatatg gaaaccctca acgacaattt aaaagtggtc gaaaaagccg
    21781 ataacgcggc gcaagtcaaa gacgcgttaa cgaagatgcg cgccgcagcg ctggatgcgc
    21841 aaaaagcaac gccgccgaag ctcgaagata aatcaccgga cagcccggaa atgaaagatt
    21901 tccgccacgg tttcgacatt ctggtcggtc agattgacga cgcgctgaag ctggcaaatg
    21961 aaggtaaagt aaaagaagcg caggctgctg cagagcaact gaaaacgacc cgcaattcat
    22021 atcacaagaa gtatcgttaa ttcctcattt ccctgttgcc tgcactcagg taacagggaa
    22081 cttatcatcg caaatgatgc accacctgat tgctgctgcc gcgccagatc agggaagggt
    22141 cttttaaatc ctgcacaaat ttgccgtcga ccagcacgtt aatcaaatca acaacctgca
    22201 tttgcgcagc gttgagttcg tcgagtttat agcctgtcca cacccagatg tctttacccg
    22261 gacactcggc gcggatgcgt tgtaccagtt tcagaatatc cggcacgttt tgcggatgca
    22321 gcggatcgcc gccggagagg gaaataccct ggcgtttgat acgagtgtca ttcagatcgt
    22381 taatgatctg gtcttccatt gctttagtaa atggctgacc ggaatttacc cgccatgtgc
    22441 ttttgttata gcaaccgggg cattcatgaa cacacccgga gacaaacagg gtgcagcgag
    22501 tgccgggacc gttgacgatg tcgacaggat agtactgatg ataattcatt atttcgcctg
    22561 gcttatcagt ccgcgtgtag gctgtatcag gcaatcaacg cctgatgcga cgctgtcgcg
    22621 tcttatcagg cctacagccg ttgccaaatg taggccggat aaggcgttta cgccgcatcc
    22681 ggcaatcaac gcgggatgcg acgctttcac atcacatccg gcatttgctt agtgcagatc
    22741 aacctatctg cccatttccc aaatgtttaa cgcggcgctt aacttcttcc tgcttgccgg
    22801 cgttaaacgg acgtgcatcc gggctaccta aatatccgca cacgcggcga gttaccgaca
    22861 cacgggaggc gtcatggtta ccacatttcg ggcaggtgaa gcctttgctg gtgcactcga
    22921 actcaccggt aaagccacac tcgtagcact catcaatcgg tgtattggtg ccgtaatacg
    22981 gtacatgctg atagctgtaa tcccagacat cttccagcgc cttcaggttg tgctgaatgt
    23041 ttggatactc gccgtagcaa atgaaaccac cgttcgccag cggcgggtaa ggcgcttcaa
    23101 agtcgatctt gtcgtacggg ttcaccttct tctccacatc gaggtggaaa ctgttggtgt
    23161 agtaaccttt atcggtcacg cccggcacca cgccaaactc agcagtatcg agacggcaga
    23221 agcggtcgca caggttttca ctcggcgtgc tgtagagact gaaaccataa cccgtttctt
    23281 ctttccactg atccactgcc tgacgcagac gttcaacaat ggcgatacct ttcgcgcgga
    23341 gctgctcgtt gtcgtaaaca tgttcgccgc cgaacagcgc gttaatggtt tcgtggatgc
    23401 cgatgtaacc cagcgaaata gacgcacgac cgtttttgaa gatttcagaa atatcatcgt
    23461 cagcattgag acgcacgcca caagcacctt ccatatagag gatcggggcc acgcgcgctt
    23521 tcacgccttc gagacgagcg atacgggtca tcagagcctt acgtgccagc accagacgtt
    23581 catccagcag cttccagaag gtggcttcat cgccttttgc ttccagagca atacgcggca
    23641 ggttcaggct gatcacgccg aggttgttac gaccatcgtg gatctgctcg ccgttttcat
    23701 tttcccacac gccgaggaag ctgcggcagc ccatcggggt tttaaacgaa ccggtgactt
    23761 tcactacctg atcgtagttc aggatatccg gatacatgcg cttgcttgcg cactccagcg
    23821 ccagctgttt gatgtcgtag ttcggatcgc cttttttatg gttcagacca tcgcgaatcg
    23881 caaacaccag tttcgggaac accgcagttt tacggttttt accgaggcct gcgatacggt
    23941 tacgcaggat agattcctga atcaggcgcg actcccagct ggtgcccaga ccaaaaccaa
    24001 aggttacaaa cggcgtctga ccgttggcgg tgtgcagggt gtttacttcg tactccagcg
    24061 actggaaggc atcgtagcac tctttaatgg tacgagagtt agcgtagcct tcggcgtccg
    24121 ggatgttcca ctcttctgcg gtcttgcgat gtttgttgta gctggcggtg acaaacggtg
    24181 ccagcacttc atcgatacgg ttaatggtgg tgccgccata aatatggctg gcaacctgag
    24241 caataatctg cgcagttacc gcggttgccg tagagatcga cttcggcggt tcaatctcgg
    24301 cgttccccat cttaaagccc tgggtcagca tgcctttcag gtcgatcaac atgcagttga
    24361 acatcgggaa aaacggtgag taatcgagat cgtgatagtg aatatcgcca cgctcatgtg
    24421 cctgcaccac gtcacgcggc agcaggtgct gacgtgcata gtgtttagcc acgatcccgg
    24481 ccagcaggtc gcgctgggtt ggaatcacct tgctgtcttt gttggcgttt tcgttgagta
    24541 acgaggcgtt ggtctgctcg accagaccac ggatctcctg attcaggcga ccgcgttttt
    24601 cacgttcaat gtcgcgatcg tgacggtact cgatgtaagc acgagccagt tgtttgtatg
    24661 gacctgacat cagctggttt tcaacagcgg tctggatctc attgatatcc acctggttgc
    24721 ggccctgcat ctgctcgctg acaactgcag caacagtggc gcaataatcg gcatcatcga
    24781 cttccgctgc tttagctgca cgcagaatcg cttctttgat gcgctctgat ttaaacggca
    24841 ctttgcagcc gtctcgtttc atcacatgcg gtgtcatgat cgctccatat ttttaagaac
    24901 aggttatcca cagaaattgg gaaaggcgtt ttccggtttt ttcgtttcgt tttccggtgc
    24961 tttccgcaat cgccatccgc ttatccacaa cgccgcacct ttttcgtggg gagctgtagt
    25021 agcaattata gtcgattgat acaacatatt gggttgggac gcattttaaa gtctatatat
    25081 agtgctttgc atcaaggatg tttgaacttt ttttgatgta gctcaaagta aaaagcagag
    25141 cgtacggatg acgggcgcta cagcgttatg taaatttttt aatgaatttg ctggttgaaa
    25201 aatcaacaaa aacaacatgc tgacagacaa aaccccggaa tgaccggcat taccggggct
    25261 tagggaagat ttacttctgt aaccaccaga cagcctcaaa aggccgtaaa gtcatggcac
    25321 agggttgtgg tgaggcttct tcgtagttat gcatcacaag ctgccagttg ccgcgcattt
    25381 gccctggctg ccagggttgg atctcacggc taaggttggc aatgaccagc aaggtttgcc
    25441 ccttccattc acggcgatag caccacaata cagggctgtt tggcagcaga tcctggtaat
    25501 tgccccatgt caggatggct tcctgcttac gcagtgcgat taacttttgg taggtgtaaa
    25561 acaccgagga ctcatcggcc agcgcggctt ctacgttgat ttgttgatag ttatcgccca
    25621 ggccaatcca cggttcgcca gccgtaaacc cggcattatc gccgttgctc cattgcatgg
    25681 gcgtgcggct gttgtcacgg gatttactgg cgaggattgc caataactcg tcggcatcac
    25741 gcccatcgtt gcgcagctcg gcaaacatat tgaggctctc tacgtcgcga tagtcagtaa
    25801 tgcgcgtgaa atgcgggttg gtcatgccaa tctcttcgcc ctggtagata tacggcgttc
    25861 cctgcatgcc atgcagcacc atcgccagca tttttgccgc aggcacgcgg tattcacctt
    25921 catcaccaaa gcgagaaaca atgcgcggct gatcgtggtt acaccagaac aaggcattca
    25981 atgctacgtt gtgcattcct tgttgccagt ggcggaacaa tgttttcaac gccacaaagt
    26041 caggtttagc cagcgtccat ttttcaccac cgggataatc gaccttcagg tgatgaaaat
    26101 taaaggtcat cgacaattca ctgcctgtca gtgccgcgta tcgctggcaa tgctcaaggc
    26161 tggtggagga catttcacct acggtcatta acccgcgtgg cgtaaacaca tcgcggttca
    26221 tctcgtgcaa aaactcgtgt gctcgtggcc cgtcggtgta gaagcgacgc ccgtcgccat
    26281 ccaggtcatc agggaaacgc gggtctttgg agatcagatt caccacatcc aggcgcaacc
    26341 cgtcgacccc acgatcggcc cagaactcac agactttttt cagctctgcg cgtaccgctg
    26401 gattctccca gttgagatcc gcctgttctg gtgcaaagag atgcaaatag tactgttcgc
    26461 tttccgcatg ccagcgccac gcactaccgc caaattttga acgccagttg ttcggtggcg
    26521 tttctggttc gccatcgcgc cagatataaa actggcggta agggctttct ttgttcagcg
    26581 cctcgcgaaa ccaggcatgt tgggtagagg tatggttaaa caccatatcg agaatgatac
    26641 gaatcccgcg cgattttgcc tgcgtcacca gttcgtcaaa atcgtccagc gtgccgtagg
    26701 tgggatcaat cgccgtatag ttcgctacat cgtaaccgtt atcgacctgc ggagagacat
    26761 aaaagggggt tagccagatg gcatcaacgc ccagtttatg cagatagtcc aggcgttgga
    26821 taacgccacg taaatcgccg gtgccgctac ccgtggtgtc ctgaaaactc tttggataaa
    26881 tctggtagat aacgccgttt tgccaccagt ggggaagatt agtcattacg ttattcctgc
    26941 aaatgcgagg gggcgcaatt gcgccccgaa gaaaattaaa caatgtccag cgtgcccagg
    27001 cggtatttcc gctgatagat aaacgaggtg agtacaatcg ggatgatgat ggcgatagcc
    27061 attgccagcg caaacacctg ccagtagctc ggttgaatcg agagaattcc cggcaggccg
    27121 cctacgccga tgccattcgc cataacgccg ttcaggccgc atagcaatcc tgccagacca
    27181 gaaccaatca tcgcgcacag catcgggaag cgatatttca ggttgatgcc gtacattgca
    27241 ggctcagtga ccccaagcca ggcggagatc gccgcaggca cggagatctc gcgttcattg
    27301 tgcttgcggc tggaaatgat aatgcctatc acggcggagc cctgagcgat attcgacagc
    27361 gcaatcagcg gccacactgg cgtaccaccc atgctttgaa tcatctgcaa atcaatagca
    27421 agcgtggtct ggtgtacacc ggtgatcacc agcggggcgt acaggaagcc aaacaatgcc
    27481 gcgccaatcg gagcaaagct gccggtcatc aggtgacgta ccgcaaaggc aacgccatcg
    27541 ccaatcatgc gaccaaacgg accaatcagc gcatgggcga ggaacaccgc gaggatcagc
    27601 gaacagacgg gcaccaccac cagatagagg taatccggca cgatgcgttt aaggcgagtt
    27661 tcaataacgc ccagcgccag cccggctaac agtgccggga tcacctgcgc ctgatagccc
    27721 actttggcga tgctgaacat gccaaagtcc cacacttccg gcagctgctg cccgagcaga
    27781 taagcgttca tcagttgggg ggaaaccagt gtcacgccaa gcacgatacc aaggatcggc
    27841 gtgccgccca tttttttcac cgctgaccag caaatcccga ccggcaggta gaagaagatc
    27901 gcttcaccga tcaaccacag aaaatcgtag atcgtttgca gggaagggta catttgcgcc
    27961 agcgtttgac cgttgctcat gggcaaatcg ccgatcacat tgcgaaaacc gaggatcaaa
    28021 ccgccgctaa tcaacgcggg cagcaacggg aagaagatct ctgcgaaatg agagatcaac
    28081 tgctcatgcc atttcatatt ctgccgggcg gcttttttta cctgctcttt atcaacctgc
    28141 gcctgtccgg ttgacgctat cagtgcttga tagtaatcac ccacgttggt gccaatcacc
    28201 acctgaaatt gcccggcatt ggtgaaacag cctttcacca tggggagttg ctcaatttct
    28261 ttcggtctgg cattggccgg ttggttgagg acaaagcgca ggcgagtaat acagtggctc
    28321 accgtcgcaa tattgccgcg cccgccgacc agttcaatca accgatcgat atccgtttgg
    28381 tttattttgc tcatcataaa gccccatggc agatgacatt tttggttggc tgcagaatat
    28441 attgcgcgga aaaatttaaa aacgggaacg ttcccgaaac gcagcgaaga tcacaattta
    28501 tcgttcagga aacgatcagg acaggttggc ggggatgatg atttgttgcg gttcgctgcg
    28561 tccggttacc tgcgcgatca actggcaagc cgcctggcgt ccagcttcgg cgtaaccagg
    28621 atctacggtg acgatctccg gatgaaggaa tttcattaac ggcgtattac cgacgctcgc
    28681 cagttgcaag gtgtcgatgc gttgctcttg caggtattta cttgcgccaa gtgccagcgt
    28741 gtcggttgcg cacagtaagg cggtagtttc aggcgtaatc acttttgcta cgttctcata
    28801 gccttgcttc atagcaagcc ctggcagggc ggcaacggga tgcagtttat gcgctttgca
    28861 gaacgccagg taggcttcgt gacgtcgctt accggttgtc acgtcactgt gcggcacgcc
    28921 gagataactg atattacgat gcccctggtc atacagccgt tgcatcagga tcttgattgc
    28981 cccttcgtcg tcataacaga ccgaagcaaa gccttttgcg tcacgcgcca gcagaaccag
    29041 cgatgactgc cagtgggcta acatttcttc tgttatgcca gtaaaaccga acagcactac
    29101 gccgtcgata ttacgccgtt tcagcacccc caaatgttcg gcaactaatt gcggggaaaa
    29161 ctgactttcc atcatgattg ggtcgtaacc ttgttcatag aacgctggca gcatggtttg
    29221 aacggcgaga ttttctgaca acgaatccag acgggtaaca atgatggcga ccactttatc
    29281 gctttgccca cgcatagcgc gcgcagaacg ggaaggggaa aatccatgct gattcatcac
    29341 tgcttcaaca cgctcgcggg tgcgctggct cacgccgctt tcgttattca gcacccggga
    29401 aactgtagat ttccccacgc cgcttaagcg cgcgatgtct ttgatggtca gccgattttg
    29461 catcctgttg tcctgtaacg tgttgtttaa ttatttgagc ctaactttac ccgtgcattc
    29521 agcaatgggt aaagtctggt ttatcgttgg tttagttgcc agcaggtatt atatcgccat
    29581 agatgctacg aatattattg gattctcctt attatttgcg gcgctttttt cacttaccgg
    29641 aggttatatg gaacctgatc ccacgcctct ccctcgacgg agattaaaac ttttccggta
    29701 agcccgtctt ttcacggcgt taccggatgc gtaaggccgt gacgttttaa cgtccctgct
    29761 cagctttatt accttcaggt aaggcttcgc cacgcctgaa gacatttctg tactgtttca
    29821 gacagtgcgg agggactcct tatgtttaaa gaaattttta cccggctcat tcgccattta
    29881 ccttcccgtc tggttcatcg tgatccattg cctggcgcgc agcagacagt gaatacggtg
    29941 gtcccgccgt ccttaagtgc gcattgcctg aaaatggcgg tgatgcccga agaagaattg
    30001 tggaaaacgt tcgacaccca tccggaaggg ttaaatcagg cggaagtgga atctgcccgc
    30061 gaacaacatg gtgaaaataa attacccgca caacaaccgt cgccgtggtg ggtacattta
    30121 tgggtctgct atcgcaaccc ctttaatatt ttactcacca ttctcggcgc tatttcttac
    30181 gccacggaag atttatttgc cgcgggcgtt atcgcgctaa tggtcgctat ttcaacgttg
    30241 ctgaacttta ttcaggaagc acgttccact aaagcggcag atgccctgaa agcgatggtc
    30301 agcaataccg cgacggtgct gcgcgtaatt aacgacaaag gcgaaaatgg ctggctggag
    30361 atcccgatcg accagctggt gcccggcgat attataaaac tggcggcggg agatatgatc
    30421 ccggcagatt tacgtatctt gcaggcgcgg gatctgttcg tcgctcaggc gtcgttaacc
    30481 ggtgagtctc tgcccgtgga aaaagccgct accactcgcc agccggagca cagcaatccg
    30541 ctggagtgcg acacgctgtg ttttatgggc accaccgtgg tgagcggcac ggcacaagca
    30601 atggtgattg ctacaggtgc caatacctgg tttggtcaac tggcggggcg tgttagtgag
    30661 caggaaagcg agccgaatgc ctttcagcaa gggatcagcc gcgtcagtat gctgctgatt
    30721 cgctttatgc tggtgatggc accggtggtg ctgttaatca atggctacac caaaggcgac
    30781 tggtgggaag cggcgctgtt tgcgctttcg gtcgcggtag gcctaacgcc ggaaatgttg
    30841 ccgatgattg ttacctcgac gctggcgcgc ggcgcagtaa agctgtcgaa acagaaagtg
    30901 atcgtcaaac atctggatgc tattcagaac tttggcgcaa tggatattct gtgcactgat
    30961 aaaaccggca ccctgacaca ggataaaatt gtgctggaga atcataccga tatctccggt
    31021 aaaaccagcg aacgcgtgct gcatagcgcg tggttgaata gtcattatca gaccggactt
    31081 aaaaacctgc tcgatacagc ggtgctcgaa ggtacggatg aagagtcagc gcgctcgctg
    31141 gccagtcgtt ggcaaaaaat cgatgagatt ccgtttgatt tcgagcgtcg ccggatgtcg
    31201 gtggtagtgg cagaaaatac cgagcaccat cagctggttt gcaaaggtgc attgcaggaa
    31261 atcctcaatg tgtgttcgca ggtgcgtcac aatggcgaga ttgtgccgct cgatgacatc
    31321 atgctgcgta agattaagcg ggttactgat acgctgaatc gtcaggggct gcgcgtggtt
    31381 gcggtggcga cgaaatacct gccagcgcgt gaaggagatt accagcgggc ggatgaatcc
    31441 gacctgatcc tcgaaggata tattgctttc cttgatccgc caaaagagac aaccgctccg
    31501 gcactgaagg cattaaaagc gagtggaatt accgtaaaaa tccttactgg cgacagtgag
    31561 ttagttgcag cgaaagtgtg ccatgaagtg gggctggacg cgggagaggt ggtcattggt
    31621 agtgatattg aaacgctatc tgacgacgaa ctggcaaatc ttgcacagcg cactacgctg
    31681 tttgcccgcc tgacgccgat gcataaagaa cgcattgtga ccttactgaa gcgcgaaggg
    31741 catgtggttg gctttatggg cgatggtatt aatgatgcgc ccgccttacg cgctgcggat
    31801 atcggcattt ctgtggacgg cgcggtagat attgcccgtg aagcggctga tatcatcctg
    31861 ctggaaaaaa gcctgatggt gctggaagag ggggttattg agggacgtcg cactttcgcc
    31921 aacatgctga aatacatcaa aatgacggcg agttctaact tcggtaatgt gttcagcgtg
    31981 ctggtggcga gtgctttctt gcccttcctg ccgatgttgc cgttacactt gcttattcag
    32041 aacctgctgt acgatgtgtc acaggtggcg atcccgtttg ataacgtcga cgacgagcaa
    32101 attcaaaagc cgcagcgttg gaatccggcg gatctggggc gctttatgat cttcttcgga
    32161 ccgatcagct cgatcttcga tattctgacg ttttgcctga tgtggtgggt attccatgcc
    32221 aacacgccgg aaacgcaaac gctgttccag tcgggatggt ttgtggtggg cttactgtcg
    32281 caaacgctga ttgtgcatat gatccgcacc cgccgtgtgc cgtttattca gagctgtgca
    32341 tcgtggccgt taatgatcat gaccgtgatc gtgatgattg tcgggatcgc attgccgttt
    32401 tcaccgctgg ccagttatct gcaattacag gcgctgccgt taagctattt cccgtggctg
    32461 gttgcgattc tggcagggta tatgacatta acccagttgg tgaaagggtt ctatagccgt
    32521 cgttacggct ggcaataaag aataaactgg gcacgatagc cccgattcag attgcagaca
    32581 aagtaagctt tgttcatgcc ggatgcggcg taaatgcctt atccggccaa caaattcaat
    32641 aaattgcagg aatcacgtag gcctgataag cgtagcgcat caggcgattt tgcgtttgtc
    32701 atcagactta atccgggcat gatagcccgg atttccatca agattagcga cgaacagcga
    32761 tcgcttcgat ctcaatcttc acgtctttcg gcagacgggc aacttcaacg caagaacgtg
    32821 ccgggaaggt ggcgttgtgt tcggtgaaga aggcttcgta agtggcgttt acggttgcga
    32881 agtcgttcag atcttttaca aacacggtag ttttaacgat gtcgcccact ttcagaccag
    32941 cggcttcgac gatcgctttt acgttatcca gcgactgacg tgcctgtgca gcgacgtctg
    33001 ccggtacttc gcccgttttc ggatttaccg ggatctgacc ggaagtgatg atcatattgc
    33061 ccagatcaac gccctgtacg taaggaccga tagctgccgg tgcattttcc gtcgcgatag
    33121 ttttgctcat gatttctcct ttattacagc ggtaaaaagt ctggctcatt atagggagcc
    33181 agacttttat taccaaccgc aattaattgg ccagcaccac attatgggaa aactcttttt
    33241 cacagtattt gcatttgagc gcgatatcat tggcgcgttt tcgcacggca aagctggaag
    33301 aaaccggttc ggcatggctg atacagttgc tgttcgggca gaccagcaca ttgtcgatgc
    33361 gctccggcag acttgggcgc gatttaccca ccacttcata gttgtcgata cggttaaccg
    33421 tggcttgcgg cgcatacaat gccagttgat ctacctggtc ttcactcaaa aaggtatttt
    33481 cgattttgat cagatctttg cggcccatct cgccagaagg caggttcaga ccgatggtga
    33541 tgcgctgatc cgtttcggtc agcttgaaca gactcaacag cttaaaaccg atctgggcgg
    33601 ggatatggtc aattaccgtg ccgcgtttaa tagcttcaac ctgcaattta ttatcgtgtg
    33661 tcatctctat ttccccttac agtaccagat cgcgattcag aaccagtgcc agtaacgcct
    33721 ggcgagcgaa aatcccgttg cctgcctgct ggaagtacca ggcgtgtggc gttttatcaa
    33781 catccgtcgc aatctcatca acacgcggca gcggatgaag tactttcata ttggctttgg
    33841 cgttgtggag atcgctggcg cgaagaacaa actgcgcttt cacgttggcg tactcggacg
    33901 ggtccagacg ctctttttgc acgcgggtca tgtacaggat gtcaacttcc gccatcactt
    33961 cttcaataga gctgtgcaga ctccatgcga tccctttttc atcaagcata tccagaatgt
    34021 attgcggcat tgccagcgcg tccggcgcga tgaagtaaaa acggttgccg tcgaacttcg
    34081 ctaacgcctg agtcagggag tgaacggtgc ggccatattt caggtcacca accattgcga
    34141 cgtggagatt gtccagacgc ccctgggttt cctgaatggt gaataagtcc agcaaggttt
    34201 gcgtcggatg ttggttggag ccatcaccgg cattcagtac cggtacattg ccggaaaact
    34261 cggtggccag gcgcgccgca ccttcctgcg gatgacgcat cactatcgca tcgacgtaag
    34321 tgctgataac cgaaatggta tcggccagcg tttcgccctt tttacccagt gatgtattgg
    34381 cgctgtcgga gaagcccacc acgctggcac ccaggcggtg catggaagtt tcgaaagaga
    34441 ggcgggtacg ggtagaggct tcgaagaaac agctggcaat gactttgtgc ttcaacagct
    34501 ctggttgcgg gtttgctttc agtttcgccg ctgtcgccag caccagatta aggtcatcgc
    34561 gactaaggtc gtttatggaa atgatatgtt tctgatatag cggattagcc atcttttatc
    34621 tcctgacgcc tgggcaaaaa aaagcccctc gattgagggg ctgggaatgg gtgatcaacg
    34681 ggaagaaaaa cggcaggcca gcgtcttttt tcagacgcgg taagacaaaa tgtcgaacac
    34741 actgaaccat acatcctccc ggcaaattgt ccggcattat actcatcgtc agaagcggtg
    34801 caagcatttg atgcaatatt ttgtcagcgc aaacggttta tttgaattat aagtcaaaat
    34861 atatgcattt ttatttactg tgattctgga ggggaactgt cgttcggtat caataattgc
    34921 agacatttcc tgcttttttc tttttcactt tcacgcaatc agatatgcat tttattccat
    34981 tcatccgact tatagggcgg agtgtgaaaa cgaacggcta acactattgc ttacagctca
    35041 gggatgcgcg ctatcacttt aatttcaaaa tcaaagcctg ccagccatgt aacgcccacc
    35101 gccgtccagg ttggataagg tggcgcgcta aatatttcat ttttcaccgt catgatgtct
    35161 tcaaattgtt tttctggatc ggtatgaaag ctcgtaacat caatgatatc gtcaaaagtg
    35221 catcccgcag ctgccagggt cgcatgcaaa ttatcaaatg ccagtctgac ttgttgctga
    35281 aaatcgggtt ctggtgttcc gtcctctcga cttcctactt gcccggaaac aaacagcaaa
    35341 tcgccggaac gaatagccgc agaataacga tgttctgcat acagtgaatg tcggccagca
    35401 gggaaaacag cggttctttc taccatttgg ttatcctcaa gatttacgac atgaacagaa
    35461 gatttatatt taccaggagc cgcttttagc ggacgacgtg agtaaacaaa acccagacat
    35521 catgggaaat ggctgggctt aattgagcgt agtcggttat gcgccaaacg cgccatcaat
    35581 ggtatgcatc gcgccggtaa caaaactggc ttctggccct gctaaccatg cgaccatacc
    35641 agcgacctct tccggttgcc catgtctttt gatagccata aaactatgca acatatcgcg
    35701 cattggcccg ttggcgggat tagcgtcggt atcaattggc cctggctgga cgacgttgat
    35761 ggtgatccca cgcggtccaa aatcacgggc cagcccgcgc gccatgcctt gtagggcaga
    35821 tttactggcg gcataagcag ccatgcctgc aacaggcata cgatcgccat tcacggagcc
    35881 gatgattaag atgcgcccac cttcgggcat ctgccgggcg gcttcaacag aggcatggta
    35941 aggagcatga atattgattt tgaaaaggcg atcaatatcg tcggcattta attccagggc
    36001 atcgccaaag acgccaatac ctgcatttac caccaggata tccaatgcgc cgctcttacg
    36061 aacgacatca atgacagcgt ctctgtcagc actatctgta aatactgctg tcgctcccgt
    36121 ctcttgtgcc aggcgttcag cggcatcttt cgaccccgca taggtgaatc gtacattggc
    36181 cccatcggtg acgaaacgac gtacgatagc ggcaccgatt ccacgactgc caccgaggat
    36241 gagaactgtc ttacctgtaa aagcgcccat aaggactcct tgatttatta tgtaacatgc
    36301 attacaaaac tgttttaact ttctgtcaac aagttttgta atggtcacta aaaaacaatc
    36361 tcgcgttcca ggtcgtccca gacgtttcgc tcctgagcag gcagtctctg cggcaaaagt
    36421 gctttttcac caaaaaggtt tcgatgctgt cagtgttgct gaagttactg attatcttgg
    36481 tattaacccc ccgagcctct acgcggcttt tggcagtaag gctgggttat ttagccgtgt
    36541 actcaatgaa tatgtcggta cggaagctat tccgcttgtc gatattcttc gtgatgatcg
    36601 tccggtaggg gagtgcctgg ctgaggtatt aaaagaagcg gcgcgcagat atagccaaaa
    36661 cggcggctgc gctggctgta tggttcttga aggcattcat agtcatgatc cacaagcgcg
    36721 tgatattgcc gttcaatatt atcacgccgc agaaacggcc atttatgact atatcgccag
    36781 gctgcatcca caaagcgcac aatgtgtgac tgattttatg agtaccgtga tgtcagggct
    36841 ttctgcgaag gcacgagagg ggcactccct cgaacaactc tgtgcaactg ctgcactggc
    36901 gggggaagcg ataaaaacta ttctcaagga gtgatgctgg ccttgatccg aaaggcggga
    36961 acgcgcctgc cgataagttg tgataagaca ataattcacg cgttaaggct ggctgaattg
    37021 atcatctttt cgtataacgg tggaaatgaa acattgtttt aattaaggag tggaaaagat
    37081 gatcatcgga aatattcata atcttcagcc gtggctaccc caggagttac gccaggcgat
    37141 tgagcatatc aaagcacacg ttacggcaga aacgccaaag ggcaagcacg atatcgaagg
    37201 taatcgtctg ttttatctta tctcggaaga tatgaccgag ccgtacgaag ctcgccgtgc
    37261 ggagtaccat gcccgctatc tcgacattca gattgtgtta aaaggtcagg aaggcatgac
    37321 cttcagcaca caaccggcag gtacgccgga taccgactgg ttagctgata aagacatcgc
    37381 atttttgccg gaaggcgttg atgagaaaac agttatcctt aatgaaggtg attttgttgt
    37441 gttttatccg ggggaagtgc ataaaccgct gtgcgcagtg ggtgcaccag cccaggttcg
    37501 caaagcagta gtgaagatgc tgatggcgtg atgacttttc gccgtaaata actccaggtt
    37561 tacggcgagt ttgtgaaaag agcgtttttt gatatttttt tgtgagtaaa atttgtaatg
    37621 cttagacgtt cttattaact caaggagttc gtcatgagca aaatatcaga tttgaattat
    37681 tctcaacaca ttacattagc cgacaatttt aaacaaaaaa gtgaagtttt aaatacctgg
    37741 cgtgttggaa tgaataattt tgcccgtaat gccggggggc a
//
LOCUS       DAZKIP010000033        37420 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZKIP010000033 DAZKIP010000000
VERSION     DAZKIP010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.033"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            219..1592
                     /gene="aroP"
                     /locus_tag="ACHOUK_001925"
     CDS             219..1592
                     /gene="aroP"
                     /locus_tag="ACHOUK_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFP9897388.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVTVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAVVLVIMLMTPGMAISVYLIPVWLVVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1635..2489)
                     /gene="ampE"
                     /locus_tag="ACHOUK_001926"
     CDS             complement(1635..2489)
                     /gene="ampE"
                     /locus_tag="ACHOUK_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFP9897389.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFRTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2486..3037)
                     /gene="ampD"
                     /locus_tag="ACHOUK_001927"
     CDS             complement(2486..3037)
                     /gene="ampD"
                     /locus_tag="ACHOUK_001927"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFP9897390.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            3125..4018
                     /gene="nadC"
                     /locus_tag="ACHOUK_001928"
     CDS             3125..4018
                     /gene="nadC"
                     /locus_tag="ACHOUK_001928"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFP9897391.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4221..4661
                     /gene="ppdD"
                     /locus_tag="ACHOUK_001929"
     CDS             4221..4661
                     /gene="ppdD"
                     /locus_tag="ACHOUK_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFP9897392.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            4671..6056
                     /gene="gspE"
                     /locus_tag="ACHOUK_001930"
     CDS             4671..6056
                     /gene="gspE"
                     /locus_tag="ACHOUK_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFP9897393.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLAGFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQKLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPVNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6046..7248
                     /gene="hofC"
                     /locus_tag="ACHOUK_001931"
     CDS             6046..7248
                     /gene="hofC"
                     /locus_tag="ACHOUK_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFP9897394.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQRAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGIMLTIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNIAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7283..8326)
                     /gene="guaC"
                     /locus_tag="ACHOUK_001932"
     CDS             complement(7283..8326)
                     /gene="guaC"
                     /locus_tag="ACHOUK_001932"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFP9897395.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIIS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(8482..8526)
                     /locus_tag="ACHOUK_001933"
     CDS             complement(8482..8526)
                     /locus_tag="ACHOUK_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9897396.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8551..9171
                     /gene="coaE"
                     /locus_tag="ACHOUK_001934"
     CDS             8551..9171
                     /gene="coaE"
                     /locus_tag="ACHOUK_001934"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFP9897397.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9171..9914
                     /gene="zapD"
                     /locus_tag="ACHOUK_001935"
     CDS             9171..9914
                     /gene="zapD"
                     /locus_tag="ACHOUK_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFP9897398.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDCQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            9924..10121
                     /gene="yacG"
                     /locus_tag="ACHOUK_001936"
     CDS             9924..10121
                     /gene="yacG"
                     /locus_tag="ACHOUK_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFP9897399.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10212..10610)
                     /gene="mutT"
                     /locus_tag="ACHOUK_001937"
     CDS             complement(10212..10610)
                     /gene="mutT"
                     /locus_tag="ACHOUK_001937"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFP9897400.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     KWMSLVGLNADDFPPANEPVIAKLKRVYVG"
     gene            complement(10670..13375)
                     /gene="secA"
                     /locus_tag="ACHOUK_001938"
     CDS             complement(10670..13375)
                     /gene="secA"
                     /locus_tag="ACHOUK_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFP9897401.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13437..13949)
                     /gene="secM"
                     /locus_tag="ACHOUK_001939"
     CDS             complement(13437..13949)
                     /gene="secM"
                     /locus_tag="ACHOUK_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFP9897402.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
     gene            complement(14180..15097)
                     /gene="lpxC"
                     /locus_tag="ACHOUK_001940"
     CDS             complement(14180..15097)
                     /gene="lpxC"
                     /locus_tag="ACHOUK_001940"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFP9897403.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15198..16349)
                     /gene="ftsZ"
                     /locus_tag="ACHOUK_001941"
     CDS             complement(15198..16349)
                     /gene="ftsZ"
                     /locus_tag="ACHOUK_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFP9897404.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16410..17672)
                     /gene="ftsA"
                     /locus_tag="ACHOUK_001942"
     CDS             complement(16410..17672)
                     /gene="ftsA"
                     /locus_tag="ACHOUK_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFP9897405.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17669..18499)
                     /gene="ftsQ"
                     /locus_tag="ACHOUK_001943"
     CDS             complement(17669..18499)
                     /gene="ftsQ"
                     /locus_tag="ACHOUK_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFP9897406.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18501..19421)
                     /gene="ddlB"
                     /locus_tag="ACHOUK_001944"
     CDS             complement(18501..19421)
                     /gene="ddlB"
                     /locus_tag="ACHOUK_001944"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFP9897407.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRVEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSVRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19414..20889)
                     /gene="murC"
                     /locus_tag="ACHOUK_001945"
     CDS             complement(19414..20889)
                     /gene="murC"
                     /locus_tag="ACHOUK_001945"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFP9897408.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(20943..22010)
                     /gene="murG"
                     /locus_tag="ACHOUK_001946"
     CDS             complement(20943..22010)
                     /gene="murG"
                     /locus_tag="ACHOUK_001946"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFP9897409.1"
                     /translation="MSAQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(22007..23251)
                     /gene="ftsW"
                     /locus_tag="ACHOUK_001947"
     CDS             complement(22007..23251)
                     /gene="ftsW"
                     /locus_tag="ACHOUK_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFP9897410.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23251..24567)
                     /gene="murD"
                     /locus_tag="ACHOUK_001948"
     CDS             complement(23251..24567)
                     /gene="murD"
                     /locus_tag="ACHOUK_001948"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFP9897411.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGVQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24570..25652)
                     /gene="mraY"
                     /locus_tag="ACHOUK_001949"
     CDS             complement(24570..25652)
                     /gene="mraY"
                     /locus_tag="ACHOUK_001949"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFP9897412.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLVGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25646..27004)
                     /gene="murF"
                     /locus_tag="ACHOUK_001950"
     CDS             complement(25646..27004)
                     /gene="murF"
                     /locus_tag="ACHOUK_001950"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFP9897413.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIVWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFAEKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(27001..28488)
                     /gene="murE"
                     /locus_tag="ACHOUK_001951"
     CDS             complement(27001..28488)
                     /gene="murE"
                     /locus_tag="ACHOUK_001951"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFP9897414.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKGEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQDATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEDRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28475..30241)
                     /gene="ftsI"
                     /locus_tag="ACHOUK_001952"
     CDS             complement(28475..30241)
                     /gene="ftsI"
                     /locus_tag="ACHOUK_001952"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFP9897415.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30257..30622)
                     /gene="ftsL"
                     /locus_tag="ACHOUK_001953"
     CDS             complement(30257..30622)
                     /gene="ftsL"
                     /locus_tag="ACHOUK_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFP9897416.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30619..31560)
                     /gene="rsmH"
                     /locus_tag="ACHOUK_001954"
     CDS             complement(30619..31560)
                     /gene="rsmH"
                     /locus_tag="ACHOUK_001954"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFP9897417.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31562..32020)
                     /gene="mraZ"
                     /locus_tag="ACHOUK_001955"
     CDS             complement(31562..32020)
                     /gene="mraZ"
                     /locus_tag="ACHOUK_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFP9897418.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(32622..33626)
                     /gene="cra"
                     /locus_tag="ACHOUK_001956"
     CDS             complement(32622..33626)
                     /gene="cra"
                     /locus_tag="ACHOUK_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFP9897419.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(33708..33794)
                     /locus_tag="ACHOUK_001957"
     CDS             complement(33708..33794)
                     /locus_tag="ACHOUK_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFP9897420.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(33806..34297)
                     /gene="ilvN"
                     /locus_tag="ACHOUK_001958"
     CDS             complement(33806..34297)
                     /gene="ilvN"
                     /locus_tag="ACHOUK_001958"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFP9897421.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLYKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(34300..36024)
                     /gene="ilvI"
                     /locus_tag="ACHOUK_001959"
     CDS             complement(34300..36024)
                     /gene="ilvI"
                     /locus_tag="ACHOUK_001959"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFP9897422.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGIG
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGGAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPQELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            complement(36342..37286)
                     /gene="leuO"
                     /locus_tag="ACHOUK_001960"
     CDS             complement(36342..37286)
                     /gene="leuO"
                     /locus_tag="ACHOUK_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308107.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFP9897423.1"
                     /translation="MPEVQTDHPETAEFSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKLNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         9048 a        10429 c         9123 g         8820 t
ORIGIN      
        1 ctgagcttta caaacggttt ctttttaagc aactcatctt caaccatgca taaagcgggt
       61 gcattcgctg ccgcatacca ttattcttga tctgacggaa gtctttttgt aactattcaa
      121 acttctttga tgtaaacaaa ttaatacaac aaacggaatt gcaaacttac acacgcatca
      181 ctgcgtagat caaaaaaaca atcaccgcac gaggtttcat gatggaaggt caacagcacg
      241 gcgagcagct aaagcgcggc cttaaaaacc gccatattca gcttatcgcg ctgggtggcg
      301 cgatagggac agggttattc ctgggtagcg cctccgtaat acagtccgca gggccaggga
      361 ttatcctggg ttacgccatt gctggtttta tcgcctttct gatcatgcgt cagctgggtg
      421 aaatggtggt cgaagaacct gtcgcaggct cctttagcca ctttgcttat aaatactggg
      481 gcagctttgc tggcttcgct tctggctgga actactgggt actgtacgtt ttagttgcca
      541 tggcagagct gactgctgtg ggtaaataca ttcagttctg gtatccggaa atcccaacct
      601 gggtttctgc cgccgtgttc tttgtggtga ttaacgccat caacctgacc aacgtaacag
      661 tgtttggtga gatggagttc tggtttgcca ttatcaaagt tattgcggta gtagcgatga
      721 tcatcttcgg cggctggctg ctgttcagtg gtaacggcgg tccgcaggca agcgttagca
      781 acctgtggga tcagggcggt ttcctgccgc acggcttcac cgggctggtg atgatgatgg
      841 cgattatcat gttctcgttc ggtggtctgg aactggtggg gatcaccgca gcagaagctg
      901 ataacccgga gcaaagtatc ccgaaagcaa ctaaccaggt tatctaccgc atcctgattt
      961 tctatattgg ttcgttagcc gttctgctct cactgatgcc gtggacccgc gttaccgccg
     1021 ataccagtcc gtttgtgctg atcttccacg agttaggcga tacctttgtg gcgaatgcgc
     1081 tgaacatcgt ggtactgact gcggcgctct ccgtgtacaa cagctgcgta tattgcaaca
     1141 gccgtatgct gtttggtctg gcacaacagg gtaacgcgcc aaaagcgctg gcgtctgtcg
     1201 ataaacgcgg cgtaccggtt aacaccattc tggtgtctgc gctggttaca gcattgtgcg
     1261 tattgattaa ctatcttgct ccggaatccg catttggcct gttaatggca ctggtggtat
     1321 ccgcactggt gatcaactgg gcgatgatca gtctggcgca tatgaagttc cgtcgcgcca
     1381 agcaggaaca aggcgtggta actcgcttcc ctgctctgct ttatccgctg ggtaactgga
     1441 tctgcctgct gtttatggcg gtggtactgg tgattatgct gatgacccca ggaatggcga
     1501 tttcggtata cctgatcccg gtatggctgg tggtgttagg tatcggctat ctgtttaaag
     1561 agaaaaccgc aaaagccgta aaagcacatt aatctctcta cgccctcacc cgtacagggt
     1621 gagggcaata atctttacac caacgccccg taaatcgtca gtagcgcaat caccaccacg
     1681 accacgaacg aggttttctt cgccattgaa accgctgcct tcggcgtctc caccttatcg
     1741 acatgcggtt cacgcgccag agagaactgc gccagacgcg ttaatacctg atattgcgaa
     1801 gtacggaaat cacccagcga agcaaaccag gccggtaacg ctttctcacc atgaccgatc
     1861 aaggcatata ccacacctgc aagacgaacc ggcacccaat ccagtacatg aagcactgca
     1921 tcaatgccgg actgtaaacg atgatgcggc gtctggtatc gcgccagcca gtattgccat
     1981 gcacgcaaaa acgcataccc catcagcgta acgggtcccc aggttccccc cacaatcagc
     2041 cagaacagcg gtgcaagata aaaacgaaag ttaatccaca gcaatgcatt ttgcagctca
     2101 cgcaaatact cacgttcgtc gcagcctgcc gggacgccgt gaatcatggt gagttcgcca
     2161 gccatcgtgg cacgggcatg gctatcatta cgtgaagcag ctgtcagata agcatgataa
     2221 tgaagacgaa ctttacctgc gccaatacac agcaaaccaa tcagcagcca caccagcagc
     2281 gtgggaacgt tgaacaatac tccctgcaat gcgcgtaaca gtaaaaaagt cacgcccatc
     2341 gcaataatgg tcatgcctaa cgtgcgcccg agagaaaaat gtttcacccg ccgaaagaac
     2401 gcttcaagac gatgatcaag ctgccagtgc tcgcccaact taaacaggcg ctcgaaaatt
     2461 aacaccagta aggttgtaaa tagcgtcatg ttgtctcctt gctgaccagc gcacgaaacc
     2521 gtgcccaatc aaatgcagga ccgggatcgg ttttccgatc cggcgcaata tcacaatggc
     2581 ccgtcatgtt attagcgata tccggatagc aatcaatcag tgcccgcgta accgccgcaa
     2641 gctgttgata ctgcgcatcg gtatacgcca gcgtatcggt gccttcaagc tcaatcccaa
     2701 tagaaaaatc attgcagcgt tcgcgcccct gatactgaga gactcccgca tgccatgcac
     2761 gtttatcgaa aggaacatac tggactattt caccatcacg gcgaatcaaa cagtgagcgg
     2821 agacgcgcaa atgggcgatc tcagcaaaga aaggatgtgc ctgcggatca atagttccag
     2881 tgaataatgc gtcgatccac ggaccgccaa actcgcctgg cggcaggcta atattgtgca
     2941 ccaccagcag ggtgggtgtt tcgtcatccg ggcggcaatc gtaatgtggt gagggaacgc
     3001 ggcgcgcgcc aaccagccac ccctgttcta acaacatgca ggatctcctt atatgtggtg
     3061 ctaatacccg gttcagagta gcatgtttct accttatgat tcgttagcta tctggagttt
     3121 taacatgccg cctcgccgct ataaccctga cacccgacgt gacgagctgc tggaacgcat
     3181 taatctcgat atccccggcg cggtggccca ggcgctgcgg gaagatttag gcggaacagt
     3241 cgatgccaac aatgacatta cggcaaaact tttaccggaa aattctcgct ctcatgccac
     3301 ggtgatcacc cgcgagaatg gcgtattttg cggcaaacgc tgggttgaag aggtgtttat
     3361 tcaactggca ggcgacgatg tcaccataat ctggcatgtg gatgacggcg atgtcatcaa
     3421 cgccaatcaa cccttgttcg aacttgaagg cccatcccgc gtgctgttaa cgggcgaacg
     3481 cactgcgctt aattttgtgc aaaccctttc aggagttgcc agtaaggtac gccactatgt
     3541 cgaattactg gaaggcacca acacgcagtt gttggatacg cgtaaaacct tacccggtct
     3601 gcgttcagcg ctgaaatacg cggtactttg cggcggcgga gcgaatcacc gtctggggct
     3661 ttctgatgcc ttcctgatca aagaaaacca tattattgcc tccggctcag tgcgccaggc
     3721 ggtcgaaaaa gcgtcctggc tgcacccgga tgcgccagta gaagtcgaag tagagaatct
     3781 ggaagaactt gatgaagccc tgaaagcagg agccgatatc atcatgctgg ataacttcga
     3841 aacagaacag atgcgcgaag ccgtcaaacg caccaacggc aaggcgctac tggaagtgtc
     3901 tggcaacgtc actgacaaaa cactgcgcga atttgccgaa acgggcgtgg actttatctc
     3961 cgtcggtgcg ctaactaagc acgtacaggc actcgacctt tcaatgcgtt ttcgctagtt
     4021 gctgacagat ttatccgctc catagtgggc ggataatctc cccttcccca accttcttcg
     4081 taacgcctcg caaatttctt gtctttcgct gcaacgaaat aaaaaacctc ggaagcagct
     4141 ttcccgtttc gttttttgcc cctcgcctgt gctgatggcg tttgccaaag tagcaccaac
     4201 caaatcaagg agcgaaacag atggacaagc aacgcggttt tacacttatc gaactgatgg
     4261 tggttattgg catcattgcc attttaagcg ccattggtat tcccgcttat caaaactacc
     4321 tgcgcaaagc cgcactcacc gacatgctac aaacctttgt gccttaccgt accgccgtag
     4381 agttgtgcgc gctggaacat ggtggattag atacctgcga cggtggcagc aatggcattc
     4441 cctcgcctac caccacccgc tatgtttcag ccatgagtgt ggcaaagggc gtggtgtcgc
     4501 tgaccgggca agaaagtctc aatgggctaa gcgtcgtcat gacgccaggt tgggataacg
     4561 caaacggcgt caccggctgg gcgcgcaact gcaatattca aagtgacagc gcattgcagc
     4621 aagcctgcga agatgtcttc cgctttgatg acgccaacta aggagcggca atgaatattc
     4681 cacagctcac ggccctgtgc ctgcgttatc agggagtctt gctggatgcc agcgaagaag
     4741 tggttcatgt tgcggtggtc gatgccccct cacatgagtt gctggacgca ttgcatttcg
     4801 ctaccaccaa acgtattgag atcacctgct ggacgcgcca acaaatggaa ggtcacgcca
     4861 gtcgcacaca acagacattg cccgtagctg ttcaggagaa gcatcagccc aaagcagagt
     4921 tgctgactcg aacgttacaa tctgcgctgg aacaacgcgc gtctgatatt catatcgaac
     4981 cagcggacaa tgcctaccgc atccgcttgc gtatcgacgg cgtattgcat cctttaccgg
     5041 atgtttcacc ggatgccgga gtcgcattaa ccgccagatt aaaagtgctg ggaaacctgg
     5101 atattgcgga acatcgcctg ccgcaggacg ggcaattcac tgtcgaactg gcaggaaacg
     5161 ccgtctcatt tcgtattgcg accttaccat gtcggggtgg tgaaaaggtg gtattaaggt
     5221 tgttacagca ggtgagccag gcactggatg ttaacacgct gggaatgcag ccgttacaac
     5281 tggcgggctt tgctcatgcc ttgcaacaac cacagggact ggtgctggta actggcccta
     5341 ccggcagcgg caaaacggtc acgctttata gtgccctgca aaagctgaat accgctgaca
     5401 ttaatatttg tagcgtcgaa gatccggttg agatccccat agccggacta aaccagacgc
     5461 aaatccatcc gcgtgccgga ctcacctttc agggcgtttt gcgtgcgtta ttgcgccagg
     5521 atcctgacgt catcatgatc ggagagatcc gcgatggcga aacggcagaa attgccatta
     5581 aagccgcgca aaccggtcac ctggtgttgt ctaccctaca cactaattcc acctgcgaaa
     5641 cgctggtacg tttacagcaa atgggggtcg cccgctggat gctatcatcg gcgcttacgc
     5701 tggtaatagc ccagcgtctg gtacgcaaac tttgcccaca ttgtcgccgg cagcaagggg
     5761 agcccatcca cattccagtc aatgtatggc cgtcgccgct gccccactgg caagcacccg
     5821 gttgtgtaca ttgctaccac ggtttttatg gtcgcactgc cttatttgaa gttctgccca
     5881 taacaccggt catacgtcag cttatttccg ctaataccga cgttgaatcg ctggaaacgc
     5941 acgcccgaca ggcgggtatg cgaacgcttt ttgaaaacgg ctgcctggcc gtggagcaag
     6001 gcttaaccac ctttgaagag ttaatccgcg tattggggat gccgcatggc gagtaagcaa
     6061 ctctggcgct ggcatggcat aaccggcgac ggcaatgcgc aagatgggat gctatgggca
     6121 gagagccgta ctttactgct tatggcacta cagcaacaga tggttacccc actaagcctg
     6181 aagcgaattg ctatcaattc tgcgcagtgg cgaggagata aaagcgcgga agtcattcat
     6241 caactggcga cgctacttaa agccgggtta acgctttctg aagggctggc actgctggcg
     6301 gaacagcatc ccagtaagca atggcaagcg ttgctgcaat ctctggcaca cgatctcgaa
     6361 cagggcattg ccttttccaa tgccttatta ccctggtcag aggtatttcc gccgctctat
     6421 caggcgatga tccgcacggg tgaactgacc ggtaagctgg atgaatgctg ctttgaactg
     6481 gcgcgtcagc aaagagccca gcgtcagttg accgacaaag taaaatcagc gttacgttat
     6541 cccatcatca ttttagcgat ggcaatcatg gtggttgtgg caatgctgca ttttgttctg
     6601 ccggagtttg ccgctatcta taagaccttc aacaccccac taccggcact aacgcagggg
     6661 atcatgacgc tggcagactt tagtggcgaa tggggctggc tgctggtgtt gttcggcatt
     6721 atgctgacga tagccaataa gttgctgatg cgccgcccga cctggcttat cgcgcggcag
     6781 aaattgctgt tacgcatccc gattatgggg tcactgatgc ggggacaaaa actcacgcag
     6841 atctttacga ttctggcgct gactcaaagt gcaggcatta cttttttaca gggcgtagag
     6901 agcgtcagag aaacaatgcg ctgcccgtac tgggtgcaac ttctgacaca aatccagcac
     6961 gatatcagta acggtcatcc catctggctg gcgttaaaaa atgccggtga gtttagcccg
     7021 ctctgtttgc aattagtgag aacgggagag gcctccggct cgctggatct catgttagac
     7081 aacatcgccc atcatcatcg ggataacaca atggcgctgg cggataacct agcagcctta
     7141 ctggaaccgg cgttgctgat cataacggga ggaattatcg gtacgctggt ggtggcgatg
     7201 tatctgccaa ttttccattt aggcgatgcg atgagtggga tgggataacg ctggcgtgtt
     7261 gctccacacc agcgttggaa gattacaggt tgttgaagat gcggttttct tgttcctgca
     7321 cacggataaa tgtggtgcgc ttggtcagtt ctttcaggcg tgaagcccca acgtatgtac
     7381 aagctgaacg caggccgccc aaaatatcac gcgcggtatt ttcaaccggg cctcgcagcg
     7441 gcagcttaac ggttttacct tctgctgcgc gatattcagc aacgccgcca acgtgacgtt
     7501 tcatcgcaga ctcggagctc atgccgtaga acagcataaa tttctcgccg ttctcctcaa
     7561 cgatgcgacc gccgctctct tcgtggcccg ccagcatgcc gccaagcatg acgaaatcgg
     7621 caccgccacc aaaggctttc gccacatcgc ccggcgtggt gcagccacca tcgctgatga
     7681 tcattccgcc cagaccgtgc gcagcatcgg cacattcgat taccgcagaa agttgcggat
     7741 aaccgacgcc tgttttgacg cgagttgtac aaacagaacc tgggccaatg ccaactttaa
     7801 cgatatcggc acctgagagg ataagctcct cacacatttc accagtcact acgttaccag
     7861 cacaaatggt tttggtcggc cacgcttcac gcgctttcgc aacgaactgc acgaagtgtt
     7921 cggaataacc attcgccacg tcaatacaaa cgaagtttaa tgccgggttc aggtcgagaa
     7981 tctgtttagt tttttcgaaa tccgcatcag acgtaccggt agaaaccatc acatgtttca
     8041 gcacatcagc ggaagaattg ttgataaacg cttgccactc ttcgacagaa tagtgtttat
     8101 gcacagcagt caaaatatca aaagaagcca gcgcagaggc catagaaaat gtgcctacgg
     8161 tgtccatatt tgcggcgata atcggcacgc cggaccagct ctgacctgaa tgtttgaagg
     8221 tgaattgacg ttccagttca acatcggaac ggcttttaag agtggagcgt ttagggcgga
     8281 tgagaacgtc tttaaaacct aacttcagat cttcttcaat acgcatgtgc ggattcctgg
     8341 ggttaatggc tataagggta agagcgcaac tccagtgacg ctatcatacg cactaatcaa
     8401 tgcggcgcaa gactgcgaaa ttgtcttttt ttacgctaca atcccataaa tttacctggt
     8461 aaatagtgag cgaacgtttc atcaatttcg ctttcgtgcg ctcattttta acgttttgat
     8521 aatcatgatg aattcccggg aatataaatt atgaggtata tagttgcctt aacgggaggc
     8581 attggcagtg gcaagagtac cgttgccaat gcgtttgctg atctcggaat taacgtcatt
     8641 gatgccgata ttattgcgcg tcaggtggtt gaaccaggtg cacctgcgct acatgccatt
     8701 gctgatcact ttggcgctaa tatgattgct gctgatggaa cattgcagcg ccgggccttg
     8761 cgcgagcgga tcttcgccaa cccggaagag aaaaactggc ttaacgccct gctgcatccg
     8821 ctgattcagc aagagacgca acaccagatc cagcaagcta cttcccccta tgtactgtgg
     8881 gttgtgccat tgctggtaga aaactcactg tataaaaaag cgaatcgagt gctggtggtg
     8941 gatgtcagcc cagaaacgca acttaagcgc accatgcagc gcgatgatgt aactcgcgag
     9001 catgtcgaac aaatccttgc tgctcaggca acgcgcgaag cccgccttgc cgtggcagat
     9061 gacgtcattg ataataacgg cgcaccggat gctatcgcat cggatgttgc ccgcctgcac
     9121 gcacactatt tgcagcttgc gtcgcagttt gtctcacagg aaaaaccgta atgcagaccc
     9181 aggtcctttt tgaacatcca ctaaatgaaa aaatgcgtac atggctgcgc attgagtttt
     9241 tgattcagca actcaccgtt aatttaccca tcgttgacca cgctggcgcg ctgcatttct
     9301 tccgtaatgt tagtgaatta ctggatgttt tcgagcgcgg cgaagtccgc actgagctgt
     9361 tgaaagaact tgaccggcag caacgtaaac tccagacctg gattggcgtg cctggcgtgg
     9421 accagagccg tattgaagca ttaattcagc agttaaaagc ggcggggagc gtattaattt
     9481 ccgcgccgcg tatcgggcaa tttctgcgtg aagatcgttt gattgctctg gtgcgtcagc
     9541 gactgagcat cccaggcggc tgttgcagct ttgatttacc tacattgcac atttggctgc
     9601 atctacccca ggcgcagcgc gactgccagg tagaaacctg gattgccagc ctgaacccgc
     9661 tcacccaggc acttaccatg gtgctggatt taattcgcca gtcggccccc ttccgtaaac
     9721 aaaccagcct gaatggtttt tatcaggata acggtggcga tgccgacttg ctgcgcctga
     9781 atctgtcgct cgattcacag ctttatccgc aaatttccgg acataagagc cgttttgcca
     9841 ttcgttttat gccgctggac actgaaaacg gacaggtacc ggaacgtctg gatttcgaac
     9901 tggcctgttg ctaaggagta aaaatgtcag aaactattac ggtgaattgc ccaacctgcg
     9961 ggaaaacggt ggtgtggggt gaaatcagcc cgtttcggcc attttgctcc aaacgttgtc
    10021 agctgatcga cctcggagaa tgggctgctg aagaaaaacg aatccccagc agtggcgatc
    10081 tttccgaaag cgatgactgg agcgaagagc caaagcagtg acatttgtct gatgccgcac
    10141 gtaggcctga taagacgcgg acagcgtcgc atcaggcatc ttgtgcaaat gtcggatgcg
    10201 gcgtgaacgc cttatccgac atacacacgc ttaagcttcg caattaccgg ttcattggct
    10261 ggcggaaaat catcggcatt aagaccgact agcgacatcc acttaccggg ttgcccttct
    10321 ttaccccacg gctccccttc ccagctttcg accagccaga accacagagt tatatgcctg
    10381 tccgggaatt catattccag tttttcaaat agcgaaaaat gttgtggggt aatcccgact
    10441 tcttcctgaa gttcacgcac caccgcctgt tccggcgttt cacccatttc aattttaccg
    10501 ccgggaaact ccagtttatt cgccatgtgc gcatctgctg cgcgacgcgt tataaagatt
    10561 tcattgttct cgttgcgaat aatacctacc gcaatttgca gctttttcat tgtcttaaac
    10621 ctataaaaaa ggcgcagaat cctgcgcctt ttacttcaac agttagcttt tattgcaggc
    10681 ggccatggca ctgcttgtat tttttaccag aaccgcacgg gcaaggatcg ttacgtccta
    10741 ctttgcgctc accggtttgc gccgccagtg cagctgcggc tgcagagtcg tcatcctgat
    10801 ggctaagctg ctgcatttgc gctaaacgct cggcttccat acgacgctgt tgttccagct
    10861 cctcaacctc ttcaggcata cgtacctgaa ctttgctcag cgtactgata acttcatatt
    10921 tcaacgactc cagcatcgct gcaaacatgg agaacgattc acgtttgtat tcctgcttcg
    10981 gatctttctg tgcatagcca cgcaggtgga taccctgacg cagatagtcc atcgctgcca
    11041 ggtgctcttt ccacagggag tcaagcgttt gcagcatgac gcctttctcg aagtgacgca
    11101 tcatctcagc accaaccact tcttctttac gctgatacac ttcgatggac tgcgccagaa
    11161 tgcgctcacg cagcgtctct tcatgcagtt ctggttcttt atccagccac tcagcaattg
    11221 gtaaatcgag gtcgaaatcg ttcttcagac gttcctgcag ccccggaata tcccacattt
    11281 cttccagcga ctgtggcgga atgtaggcat caatggtcgc tttgaacaca tcttcacgaa
    11341 tgctgttaat ggtttcgctc acatcgctga catccagcaa ttcgttacgc tgggagtaaa
    11401 tggcgcgacg ctgatcgtta gccacgtcat catattccag cagttgctta cgaatgtcga
    11461 agttacgact ttcaacttta cgctgggcgt tggcaatcgc tttagtcacc cacgggtgtt
    11521 caatggcttc gcctggcttc atacccagtt tacgcatcat gccggatact cggtcggaag
    11581 caaaaatacg catcagcgca tcttccatcg acaggtagaa acgggaagaa ccagcatccc
    11641 cctgacgacc agaacgaccg cgcaactggt tatcgatacg acgggattcg tgacgctcgg
    11701 taccgatgat atgcaggcca cctgctgcca gtaccgcatc gtgacgtacc tgccagtcgg
    11761 ctttaatttt ttcaatttgc tctgcggtcg gattttccag cgcggcaact tctgcctgcc
    11821 agctaccacc gagcacaata tctgtaccac gacccgccat gttggtcgcg atagtcaccg
    11881 cagccggata acctgcctga gcaacaatcg ccgcttcgtt ggcgtggaat ttggcgttca
    11941 ggacattgtg cttaataccg gctttggtca gttcgtttga caccagctcc gatttttcga
    12001 tggagatagt acccaccagc accggctggc ctttcgcagt acgttctttg atatcttcaa
    12061 tgatcgcctg aattttttcc gcttcagtca tgtagaccag gtccggcaga tctttacgaa
    12121 tcattggacg gttggtcgga acaacgacgg tatccagttt gtagatggag ctaaattcga
    12181 aagcttcggt atcagcagta ccggtcatcc ccgccagttt ttcatacaga cggaagtagt
    12241 tctggaaggt gatcgaagcc agcgtttggt tttcgttctg gatctgcaca ccttctttcg
    12301 cttccacagc ctggtgcaga ccatcggacc agcgacggcc ctgcatggta cgaccggtgt
    12361 gttcgtcaac gatgataact tcaccatctt taacgatgta gtcgacgtca cgggtaaaca
    12421 gcgcatgagc gcgcagcgcc gccgttacgt ggtgcatcag catgatgttg gccggagagt
    12481 acagagactc cccttcatcc atgatgccct ctttcaccag cagttcttca atcagcacca
    12541 gaccacgttc ggtcaggttc acctggcgag atttttcgtc caccgagaag tggccttcgc
    12601 cctggaaggt ttcggagtct tctttttcct gacggatcag gtgcggaata attttattca
    12661 cgcgtttata catttccgag ctgtcttctg ccgggccgga aatgatcagc ggtgtacgcg
    12721 cttcatcgat caggatggag tccacttcgt ccaccagcgc atagtgcagt ttacgctgta
    12781 cacgttcttc agggctgaac gccatattgt cgcgcaggta gtcaaagccg tattcgttgt
    12841 tcgtaccgta agtgatgtca gctgcgtaag cttcgcgctt tgccggtgct ggcatgcccg
    12901 gcaggttgat accgacagtc aggccaagga attcaaacag cggacggttg ttttcggcgt
    12961 cacgttgcgc caggtagtcg ttgacggtaa ctacatgcac gcctttaccg gttagtgcgt
    13021 tcagataagc aggcagcgtt gcggtcaggg ttttaccttc accggtacgc atttcggcga
    13081 tgcagcgttc gttaagaacc ataccgccga gtaactgaac gtcgaagtga cgcataccaa
    13141 agacgcgctt acttgcctca cgtaccacgg cgaaagcttc cgggatcaga ttttccagca
    13201 cttcgccttt ttccagacgt gcacgaaact ctgcggtttt ccctttcagt tcttcgtcgg
    13261 agagtttttc catctccggt tccatggcat tgatgatgtt gaccactttg cgcatccggc
    13321 gcagggtgcg atcgttacga ctaccgaaaa ctttagttaa taatttgatt agcataataa
    13381 aatctcaaac gccccgcgtt gcggagttaa taaaatgaag taaaggttta ttgttgttag
    13441 ctgaggcgtt gagggccagc acggatgcct tgcgcctggc ttatccagac gggcgtgctg
    13501 aattttgctt gtggggtaaa atgcgcataa tcaatgcgat aacccttttc agacggcgtg
    13561 ccttcctggg tcagcagcgc gctgagcgta tccagtaatg caagatgttg cgcctgaaga
    13621 ggcaaagatt cttcagcaac gggcagcgtt tgcggtgcca ttgcgaagga aagatgacgg
    13681 attaccgtgc gaatggcatg ttgatgccag taatctacgg aatagttcga attcgggcgg
    13741 cgcgtgttcg cttccagcaa ggccaattga ccaaagttaa ctttggctga aggctcgtgg
    13801 ttgcgggttg tcgcttttgc gggcgcgttt ggttcggcgg cgttgctgag cgcaggcaaa
    13861 cctaaactcg ccgcaaccat ccctaataag agatgcggcc agaagtagcg tttaccaaac
    13921 tgtcgccagc gcgtcagtat tccactcacg ttattgccat cccgatcgta ttcaaacgta
    13981 tttagcgcac aaatcttatc attaaagcct gaagtccaca gcacgaatga agataagttg
    14041 tccgtaaaag tgcttaagaa aacagcgttc gcaccgtttt ttatagctct tataaaattc
    14101 aaaaaaaaac gactggttca cctggccgga gagagtgcca gatttgccag tcgaatttta
    14161 tacgacagta taaatgtcgt tatgccagta cggctgaagg cgctttgaag gccaacggca
    14221 gttctgcgtc gtcctggaag gtcacatatt cccaggcttc ctgtttcgcc aggacagcct
    14281 gcagcagttt gttattcagt gcatgaccgg atttataagc ggtaaatgca ccaataatat
    14341 tgtgaccaca catgaacaag tcaccgatcg catcgagcat tttgtgacgc acaaattcgt
    14401 cttcaaaacg caggccgtct tcgttcagta cgcgataatc gtcaacaacg atggcacaat
    14461 cgaagctgcc gcccaggcac aaaccacggg actgcagata ttcgatatca cgcatgaaac
    14521 cgaacgtacg cgcacggctg atctggcgca taaacgcatc agcggagaag ttcatcgcat
    14581 agcgctggtt gctggaatca atagccggat ggttaaaatc gatggtgaaa tccagcgaaa
    14641 aaccattgta cggcttaaat tcagcccact tatcgccatc ttcgacacga acagtctctt
    14701 tgatgcgaac aaattttttg gcgcagttca actcgtcgat accggcgtca agcagcaggt
    14761 atacaaacgg agcggcgctg ccgtccatga tcgggatttc cggcgcgtta acttcgataa
    14821 caatgttatc gatgcccaag cccgcgagag cagcattgag gtgctctacg gttgaaatcc
    14881 gtacatcatg ctcgttgacc agacacgtac agagcatggt atcacgcaca gatttggcat
    14941 cggccgggaa atctaccggt ggattcaagt cggtgcgacg atagatgacc ccggtgttgg
    15001 ccggcgcagg gcgtaacgtc agggtgactt tcttgccggt atgtaaaccg acacccgtcg
    15061 cctgaacgat acgtttaagt gtcctttgtt tgatcatcgt attatctcgc caaattacct
    15121 atccaaccga agtgtactat acattcggcg ggccagttta gcacaaagag cctcgaaacc
    15181 caaattccag tcaattctta atcagcttgc ttacgcagga atgctgggat atccagataa
    15241 tccggctctt tcgcagtttg cggcgcattg tcattcacga ctttagcaac cggcttctgc
    15301 tcctgggtca gcggagccat cccatgctgc tggtagcgat ccatcactgg ctgctgaacc
    15361 tgcttattgg tcaccagagt gatttcagga cgtttgtcca tgccgatacc tgtcgcaaca
    15421 acggttacgc gcagctcgtc attcatatcc gggtcaagag aagtaccgat aaccacagtc
    15481 gcgttgtcgg aagcaaatgc acggatggtg ttacctaccg tttcgaactc atccagacgc
    15541 aggtcgaagc ccgctgtgat gttaaccagc acgccgcgtg cgccagacag gtcgatatct
    15601 tccagcagcg gagaagagat agccatttca gcagcttctt ccgcacggtc ttcaccgctc
    15661 gccacgccag aacccatcat tgcgtagccc atttcagaca ttacggtgcg tacgtctgca
    15721 aagtccacgt tcatcaaacc cggacgagta atcagttcgg cgatgccttg cacagcgcct
    15781 ttgagtacat cgttcgctgc gccaaacgca tccagcaggg agataccgcg gcccagaact
    15841 ttcagcagtt tgtcgttcgg gatagtgatc agagagtcca catgcttgga cagttcagtg
    15901 atcccctgct ccgcgaatgc catacgcttc ttgccttcaa agttgaaggg cttagtgacg
    15961 acagcaacgg tcaggatacc caaatctttt gccacttcag cgacgactgg tgctgcacct
    16021 gtaccggtac caccacccat acccgcagca ataaagacca tgtctgcacc ttccagcgcc
    16081 gcacgcagtg catcgcgatc ctcatctgcc gcattgcggc caacttctgg attagcgcca
    16141 gcgcccagtc ctttggtgat accgctaccg atttgaatcg tctgtccaac cgctgtttta
    16201 cgcagcgctt gtgcatcggt atttaccgcg aagaattcaa caccttcaat gcgctcgcgc
    16261 accatgtgtt caacagcatt accgccgccg ccgccgacgc cgatgacttt aatcaccgcg
    16321 tcattggtaa gttccattgg ttcaaacata gtttctctcc gatttgtgcc tgtcgcctga
    16381 ggccgtaatc atcgtcggcc tcataaaaat taaaactctt ttcgcagcca actattgagt
    16441 cgcttgatcc acgagccaac tgatgctgta acacgttttt ctacttcagc ttcaccgtta
    16501 agatgtgact ctttcccata gtgaagcaat cccaccgccg tcgaataata cggctcctga
    16561 gcataatccg ttaaaccggt aatgttcagc ggcgcgccga tacgcacttg cgtatgaaac
    16621 acgcgctgag cacaggctgc aagaccttcg atctgcgctg cgccaccggt taatacaatg
    16681 cctgccgcca ggtgatgttt aaccccttgt tggcgaagct tttcctgcaa ctgcaatatc
    16741 tcttcgttga ccaggttgag cagctcggta tagcgcggct cgatcacctc tgccagggtc
    16801 tgacgttgca gactccgcgg cggacgaccg cctacgctcg gcacttccac gctctcatct
    16861 tttccaacga tggaacccag cgcacaaccg tggcgaactt taatcgcttc ggcgtcgctt
    16921 ggcggcgtgc caaaggcgta agcgatatca ctggtcacga cattgccagc ataaggaatt
    16981 accttagtgt ggcgcaatgc cccaccggta taaacggcga tatccattgt accaccaccg
    17041 atatcgacga cgcagacacc cagttcacgt tcatcttccg tcaataccga ataacttgat
    17101 gccagaccgg caaatatcag ttggtcaact ttcagcccac aacgttcaac cgctttgacg
    17161 atgtttttcg ccatatcgtt gtggcatgtg atcaggtgca cttttgcctg catccgcacg
    17221 cccgaaagcc ctaccggatt cttgatccct tcctgatagt caatcgcata ctcttgcggg
    17281 atcacatgca gcacacgatg ctcatcgcgt acacgcaccg atttcgcggt atggacgacg
    17341 ttttccacat cttcttgcgt tacttcttct tcagaaatag gcaccatacc aatttcattc
    17401 tggcagctga tgtgcttacc agaaagcgcc agatataccg aagagatctg acaatctgcc
    17461 atcaattctg cctggtcaat ggcacgttgt acgcacttga ccacggattc gaggtcgttc
    17521 accccgcctt tatccatacc acgcgacggg cagctgccca cgccaatgat attgaccata
    17581 ccgtcgggca gaacttcccc tactaaagcg gcaaccttcg cggtaccaat ctccagtcct
    17641 actaccagtt ttctgtccgt cgccttgatc attgttgttc tgcctgtgcc tgattttgtt
    17701 gctgagtaga ttcctctggc ggcaagggcg cccagcctac tgccgctcca gagtcataac
    17761 gcaaatcaac gtagctaatc cgtttgccat cggtttgcgc ctgctgctgt agaaccggat
    17821 aaagttctac aaagcgagcc aaacgtttca tcgtatcgcc ccggccaaga ttgagcttaa
    17881 tatcgttatt cagcgtcaac tgccaggaac gccgcgcggt catcgccgct tccttcagag
    17941 taaatctgtc ctttgccagc atctgcccca tttcgcgata gccctgcaac acttcattgg
    18001 cgctgccttc cgggccatac agcattggaa gcacctgctt gctggtgcga tctggcggca
    18061 cgctgaaggt atttccttcc gcgtctacca tgtgttgatc attccaccgc gcaatcggca
    18121 catattcaac cagatgaatc ttcaattcat caggccactg ctttctgacg ctcacctgct
    18181 taatccacgg cagacgttgt tctatttgcg tctggatgat gttgacatcc tgggtcataa
    18241 aggtacccgg ctcacccaat gccaggatcg actgccggat atcgtcatta cgcgtgtaat
    18301 ggcgttcacc ggtcaacacc agctttgaga gcggcaggcg ttgcgcatct tccatccagc
    18361 ccaacacgac ccagccgctc accaacactg tcgttaaaac ggtcagcagg aaaaggatcc
    18421 ccgccagacg cgttccatta ttgcggcgag aagaaacctc ttcttcgctg tttcgcgtgt
    18481 tcagagcagc ctgcgacata ttagtccgcc agttccagaa ttcgtactac caactgcgag
    18541 aagctcatac ctgcctgacg tgccgccatc ggcaccaggc tgtggctggt catacccggt
    18601 gaggtattgg cttccagcag ataaaactgt ccatcgctgt ccagcataac gtcaatacgt
    18661 ccccatcctt tgcaacctaa cgtcgtccat gctttcagca ctaatgcctg caaattggcc
    18721 tcttgtgacg cttccagacc tgcggggcag aaatactgtg tctcatcaga gagatacttc
    18781 gcctcataat catagaaggt tccggacggt tgaatacgta ctgacggtaa aatttcttca
    18841 ccgagtatcg caaccgtgaa ctccggccca cttagccatt tttcaatcaa tacttcttca
    18901 tcgtgctgaa atgccaatct taatgcatct tgtagagcat tttctgctac tacttttgac
    18961 attcccacac tggaaccttc gcggctcggc ttaacgataa ccggcaaacc cagagcagaa
    19021 atttctgcta actgcttatc actcaggcct ttttcaaact ctacgcgggt taacgctacc
    19081 cacggcgcga ccggtaaacc ggcaccttgc cacagaagtt tgctgcgtag tttatccatt
    19141 gaaagcgcag atgccatcac tccgctgccg gtataaggca agcccatcag ctcgagcatc
    19201 ccctgcagcg taccatcttc accgccgcgg ccgtgtagcg cgataaacac tttctgaaag
    19261 cccatcgact tcagttgcgt cacgtcaact tctttcgggt cgacaggata tgcgtcaata
    19321 ccgccttcac gcagcccggc taacaccgct gcgccagaat tcagagaaac ttcccgctca
    19381 gcggaagtcc cgcccaacag gaccgcgatt ttatcagtca tgttgttctt cctccggagt
    19441 ttgcggcttc agtttgattt cagctaaaga acgggcaatt tttccgatat taccagcccc
    19501 ctgaacgaga atcaggtcgt taccggttaa taccggtgcc agcatctcgg ctacctgcgc
    19561 agggtccggc accagaatgg gatctatttt cccacgtcca cgaattgtgc gacacagcga
    19621 acggctgtcc gcacccggaa ttggcgcttc gccagccgga tacacttcca gcatcaacag
    19681 ggtatcaacc tgcgtcagca cattggcgaa atcatcatac aggtcgcgtg tacgggtaaa
    19741 acggtgcggc tgaaacagca ttaccaggtt tttatccggc cagcctgcgc gcgccgcttt
    19801 aatggtggcg tccacttccg tcgggtggtg gccgtagtca tcgaccagca ttgccgtacc
    19861 gcttttacca ttcactggct ccagcgggaa ttcaccgagg aaatcaaaac ggcgaccagt
    19921 cccctggaag ctctccagcg cacgcaaaat agcctcgtcg tcaatgccct cttccgtagc
    19981 aaccgcaacc gcagctgcgg cgttcagcgc gttatgacgg cctggcgcat tcagggtgac
    20041 gcgcatcggc tctttgtcct ggcgcagcag cgtaaagtgc ccctgcgggc caatctgctg
    20101 ataatcttct acacgcacgt cggcatcttc gctgaagccg taagttgtgg tctgacgtcc
    20161 cactcgcggt aacaattcgc ggatcaccgg atcatcaaca cacatcaccg cacgaccgta
    20221 aaacggcagg ttgtgcagaa aattaataaa agtctgtttt aaattctcaa agtcgccctg
    20281 gtaggtatcc atgtggtcgg cttcgatatt ggtgacaatc gccaccatcg gttgcagatg
    20341 caggaacgat gcatcactct catctgcttc ggcaatcagg taccgaccat gccccaaacg
    20401 cgcatgaacc cccgccgctt ttaccagccc gccgttaacg aaggttgggt cgagccccgc
    20461 ttctgcgtag atgctggaga ccatcgcggt agttgtcgtt ttgccgtgcg ttcctgcaat
    20521 ggcgatgcca tgacgaaaac gcattaactc agccagcatt tcggcacgac ggatcaccgg
    20581 aatacgcgct tcatgagctg cgacaatttc cgggttatcg gcagaaatgg cgctggaaac
    20641 aacgaccacg ctggcatcac gtacgttttc cgggcgatgg ttgaaataaa tcgtcgctcc
    20701 cagattcatt aactgctgcg tgaccggatt tggcgctaaa tcggaaccac tgatctgata
    20761 accttcattg gccagaactt cggcaatacc gcccataccg gcaccaccga tgccgacaaa
    20821 atgtatgtgc cgaacgcgac gcatttcggg cacgatggaa cgcagttttg ccaattgttg
    20881 tgtattcatt ctttacgcca ttaacttctt aaattcatac gatgcaaaag gcatcgctac
    20941 aattacgccc gggcagcccg actcacttca tttgccactc gctcggtggc atccggaatg
    21001 gatgcagcgc gggcgcgttc tgccatggtt aataaggttt ctcgcgacca cccggccagg
    21061 gtgttggcga cagcatccac gctaagctgt ggctgctcga taattttggc tgcgcccgct
    21121 ttttccagcg gtagcgcatt ccagtattgc tggcggtctt tatgttgaaa cggcacaaac
    21181 aacgccggta gtcctgccgc ggcgatttca ctcaccgtta aagcaccgga gcggcaaacg
    21241 acgacatccg cccacgcata cgccgccgcc atatcatcaa taaattcagt cactttatgc
    21301 tgcggttgcc ccgcttcggc atacgcctgt tcaacggatt gttgcgaacc tttgccgctc
    21361 tgatgccaga tagtgactga atcacccagt ttcgcagcaa cctgcggcat tgtctggtta
    21421 agaatgcgtg cgccctggga accacccact accagcacac gaaccggacc ttcacgtcca
    21481 gccaaacgtt gctgcggcaa cggtagcgcc aacacatcgg tacgcaccgg gttacccacc
    21541 acttccgcat tggggaaagc acctggaaac gcctgcatca ctttggtggc aatcttcgcc
    21601 agccatttat tggttaagcc cgcaataccg ttttgttcat gaagtacaac cggaatgcct
    21661 aacgaccacg cggccagacc accaggacct gatacgtagc cgcccatacc gagcaccacg
    21721 tcaggtttgt acgctttcat aatcgcccgc gcctgacgcc aggcattgaa gatacgcagc
    21781 ggcgcagcta tcagtgcttt tatacctttt ccacgcagac cagagatacg aatgaaatca
    21841 atttcgatgc catgttttgg cactaagtcc gcttccatac ggtcggcagt ccccagccag
    21901 cgaacttgcc aaccctgagc cattagatgg tgcgcaaccg ccagtcccgg gaatacatgt
    21961 ccaccggttc cgcctgccat caccattaat cgctttcctt gagcactcat cgtgaacctc
    22021 gtacaaacgc ctgcgctttc tccagacgcg tttcataatc aatacgcaac agcatcatga
    22081 tggctgtcga cataatcagt aagctcgaac caccgtaact gatcagcggc aatgtcagac
    22141 ctttggtcgg taacatcccc gccgccgcgc ctacgttaac cagcgcctgg aagctaaacc
    22201 agatgccaat agaacaggcg agaaaaccgg aaaaacggtg gtcaatttct aatgctttac
    22261 ggccaatcga catcgcgcga aaagcgacga agaataccat taaaagtgcc agcaccacac
    22321 cgacataccc cagttcttcg ccgataatgg cgaaaataaa gtcagtgtgc gcttccggca
    22381 gatactccag tttttgtaca gagttaccta aaccttgtcc ccacagttcg ccgcgaccaa
    22441 acgccatcag cgattgcgtt aactgatagc cgctgccaaa gggatcttcc cacgggttcc
    22501 agaatgcggt aacacggcgg atacggtacg gttcggcgag tatcagcaac acaaccgctg
    22561 aaatgcccat accgataatg gcaatgaact gccacaattt cgctcccgcc aggaacaaca
    22621 tcgccagcgt agtcacaaac aacaccacca ccgtaccaag gtctggctgt gccagcagta
    22681 acactgccaa caccagaatc acgcccatcg gtttcaggaa gccgcgcagg ttattacgta
    22741 cttcgtcacc tttacgcacc agatagttgg cgatatagca aaacagcgac agttttgtca
    22801 gctcagcagg ctggatacgc agcaaaccga gatcgatcca acgcgatgcc cctttaaccg
    22861 agctccccac taccagcacg atcatcagca ggatgataga tccgagcagc atcgtggcac
    22921 tgtagcgttg ccagaactcc atcggcagac gcagcgtaat gatcgccaga ataaacgcca
    22981 aaatcagata gacaccatca cgcttcgcga agaagaacgg atcgttggtt aagcgttgcc
    23041 ctatgggcat tgacgccgag gtcaccatga taaagccaat cgccgcgagg ccgaaggtca
    23101 gccacagtaa ggtgcgatcg tacatgatca ggctgtcggt atctttttcc cgcgagccca
    23161 tcacccagcc ctttagcgcc gtggagatcc agaccaggat actgaatcct ggcaggcgcg
    23221 gcattttcag gcgagggaga gataaacgca tcaacctaac tccttcgcca gacgggcaaa
    23281 ctcattgcct cgttgttcaa agttcttgaa ctgatcaagg ctggcacagg ccggggagag
    23341 tagaaccata tcgcccggct gaacacgcgg agccagcaag cgcatcgcct gttccatagt
    23401 ttcggtttgt tctgccactt ccgggcgtag cgccgccagc tgcacgccgt cacgaccgaa
    23461 acaatacaga cgtacgttat cgccattcag gtaacgcgcc agtgggctaa agtccgccga
    23521 tttaccatcg ccgcccagca acaaatgcag cgtgccgtct acgtgcaggc cattcagcgc
    23581 cgcttccgta ctgccgacgt tggtcgcttt cgaatcgtta atccaacgta cgccgttatg
    23641 ctccagcaca acttcaaagc gatgcggcag accagtgaat gtggttaacg ctttcaggct
    23701 gctggcacgc ggtaaccctg cggcatctgc cagcgccagc gccgccagcg cattggtgta
    23761 gttatgctgc ccggaaagtt tcatttcttt cacgttcagc actttctcgc ctttcacccg
    23821 cagccaggtt tcgccctgct gatgattcag gtgatagtca cccatgttga cgccaaagct
    23881 gacgcagcgt tcatccgcac cgcgaatcgg cattgttaag gcatcatcag cattgacgac
    23941 acaaactttc gcattttcgt aaatacgcaa ttttgccgca cggtattgct gtaaaccaaa
    24001 cggatagcga tccatatgat cttcagtcac gttcagaatg gtcgctgcta ccgcctgtaa
    24061 gctggaggtg gtttccagct ggaagctcga cagttccagt acgtacagtt cgcactcatc
    24121 atccagcagc atcaacgcgg gcaggccaat attgccaccc acaccaacgt taacccccgc
    24181 cgctttcgcc atttcaccca ctagcgtggt gaccgtgctt ttgccgttag aaccggtaat
    24241 cgccacaatc ggtgcttgtg cttcgcgaca gaacagctcg atatcgccaa cgatttcgat
    24301 tccggcatca gcggcagcgc ttaaggatgg atgcgccagt gcaataccgg gactggcgac
    24361 aatcagatct gccgccatca gccattcatc attcagactg cccgtgtggc gttctacggc
    24421 ttcgggtaat ttatccaggc caggcggtgt catacgcgta tccataacgc gcggcgtcac
    24481 accgcgagcg aggaaaaagt ccacgcagga aagcccggtg aggcccaggc cgataatgac
    24541 aacattttta ccctggtaat cagccatgat taacgtacct tcagcgttgc cagaccaatc
    24601 aggaccagca tcagcgaaat aatccagaaa cgcacaatga cgcgcggttc cggccagcct
    24661 ttcagttcat agtggtgatg aatcggtgcc atgcggaaaa tacgttgtcc gcgcagttta
    24721 aaggagccga cctgcaggat gacggaaagc gtttctacca cgaacacgcc ccccataatc
    24781 accagcagga attcctgacg cagcagtacg gcgataatgc ctaacgcacc acctaacgcc
    24841 agcgaaccta catcgcccat aaagacctgc gccggatagg tgttaaacca caggaagccc
    24901 agccctgccc cgactatcgc ggtacagaca ataaccagtt ccccggcgtg tcgcagatat
    24961 ggtatgtgta aatagctggc aaagttcatg ttgccggtcg cccacgccac cagcgcaaaa
    25021 ccaccggcga caaatacggt cggcataatt gccagaccgt caagaccatc ggtcaggttt
    25081 accgcgttgc cagtacccac aatgacgaag taagccagca gaatgtagaa cagccccagt
    25141 tgcggcatca catctttaaa gaatgggacc accagctgcg ttgcgggcgt gtctttgcca
    25201 acaaggtaca gcgcgaaggc gacacccagc gcaatgaccg acatccagaa atacttccaa
    25261 cgagcgatca accctttggt gtctttacgc actactttgc gataatcatc aacaaagcca
    25321 ataacaccgt aacctaccag caccaccaac acgcaccaga cgtacggatt ggacgggtaa
    25381 gcccacagca gtacggagat cacaatcgcc gtcaggatca taatcccgcc catggtcggc
    25441 gtaccgcgct tgctgaagtg tgattcagga ccgtcgttac gcaccacctg accaaaggaa
    25501 agtttttgca aatgagcaat catacgcggg cccatccaca atgagatgaa cagcgcggtc
    25561 agcaggctga cgatggcgcg aaacgtcaga taggaaaaga cgttaaagcc ggaataatat
    25621 ttgaccaaat gttcggccag ccaaactaac atgtcccatt ctcctgtaaa gcgcgtacta
    25681 cctcttccat ggcggcacta cgtgaaccct taactaaaat cgtaattacc tgttgctcag
    25741 caatcagtga tttaagacgc gtaattaacg cagttttctc agcaaaatgt tcgccaacgc
    25801 cgctggcggt gctgatagca tggctttgtt tacccacgct taacacgcgg tcaataccag
    25861 cagcttttgc cgcctcgccc acctgtacat ggcaggcttc gctttcagcg cccagttccg
    25921 ccatatcgcc caccaccagc acgcggtagc ccggcatttc agccagtacc tgaacagcgg
    25981 cggtcattga accgacattg gcgttgtagg agtcgtcgag cagcaactgg ttttctgcca
    26041 gttgaatggg gaacaaacgg cctggaacag ctttcagatt tgccagcccc gctttgatag
    26101 catcaagcgt tgcgcccacg gacatggaga gcgcagcggc tgccagcgca ttcgcaatat
    26161 tgtgacgccc cggcaacggc agcagaacat ccacgctacc ggttggggtt tgcagggtaa
    26221 attccgtacc gtgcgaagtc acatggatat tggtggcggt gaaatcgctg ttggcggcat
    26281 taggtgagaa acgccacact ttgcgtgagc caattacgct ctgccagttc agccagtcgt
    26341 tgttgtcggc gttcataatg gcgataccgt tttccggcag gccgctaaag atttcacctt
    26401 tcgctttcgc gacacccgca agcgagccaa aaccttccag atgcgccgct gccaggttgt
    26461 tgaccagcgc agcttccggg cgagtcagac tcacagtcca gacaatttcg ccctgatggt
    26521 tcgcgccaag ttcaataact gcgtaatcgt attccggcgt taagcgcaac agcgtcatcg
    26581 gtacaccgat gtcgttgttg agattgcctg ccgtataaag cgtgttgccg cactggctta
    26641 aaatcgccgc cgtcatctct ttaacggagg ttttgccgga ggaacctgtc agagcaacca
    26701 cgcgcgccgg aacttgctgg cgaacccatg cagccagttc accaaacgcc agacgcgtat
    26761 ccttgacgat taactgcggc aggtcgatat ccagcggacg gctaaccagt agtgcgcctg
    26821 cgccgccagc tttcgcctgg tcggcaaaat catgagcatc gaaacgttcg cctttcaggg
    26881 caacaaacag gcagcccggc gtcagttttc gggtatcagt ggttacagca tcaagggtga
    26941 tatctgcacc ttgcagttca ccgttgagaa tgtcggtaag ttggctaagg gttacgctaa
    27001 tcatgcaatc acccccagca gacgcgccac cgtgacgcga tcggagtagt ccagacgctg
    27061 attgccaaca atctggtaat cttcatggcc tttgcccgcg accagcacca catcgttctc
    27121 tttagcctgc ataacggcgc aagtcaccgc ttcagcacgg tcttccatca ctttggcatg
    27181 tccggcatct aacattcccg ccagaatatc gttgatgatg gcacgcggct cttcggtacg
    27241 cggattatcg tccgtcacca ccgccacatc agcaaactct tcggcaattg cgcccatcag
    27301 tggacgctta cctttatcgc gatcgccacc acagccaaag acacaccaca gcttgcccgc
    27361 acagtgcaga cgcgccgcct gtaaggcttt ttccagtgca tccggcgtat gcgcgtaatc
    27421 caccaccacc gtcggtttgc ctggcgcagt gaacacttcc atacgtccgc aaaccggttg
    27481 cagacgagca gcggttttca gcagatcagc cagtggatag cccagcgcca gcagcgtcgc
    27541 cagcgcgagc agcaggttgc tgacgttaaa agcgcccatc agatggcttt caatttcgcc
    27601 atcgccccaa cttgagctaa agcgaatagt cgcaccgctg tcgtgatagt tcacttcggt
    27661 cgctttcaac cagcgtccgt gacagttcgg attgatatga tcttccattg ataccgcaac
    27721 tgcatccggc agttttgcca gccagcggcg gcctacttca tcgtcggcat taataatcgc
    27781 ctgaccgcaa tgatgttcag aataaagcag ccatttcgca gcttcgtagt gttccatatc
    27841 accatgataa tcaaggtgat cgcggcttaa gttagtaaag accgacgccg caaatttcaa
    27901 tgccgccaca cggtgctgta ccagcccgtg ggaggaaact tccattgcgc aaaaagtcgc
    27961 gtcctgatcc accagccccg ccagctcatg ctgaacatca actgccgaac cggtggtatt
    28021 ttctgtcggg atcactttcc ccagcagacc gttaccaacg gtgcccatta ccgcgctggt
    28081 ttcgccaagc agttggctcc actgcgccag cagctgggtc gtcgtggttt tgccgttggt
    28141 gcccgttacg cccacgagac gtaaattgtc agagggttca tggtaaaagc ggcccgccag
    28201 tgcagataaa cgctcgttga gctggctgag atagatgacc ggtacgccgt gcatttcacg
    28261 gatttcaccg tcggtcgcct cacctttcgc ctctgcaata atggcagcca caccttgcgc
    28321 tatcgcctgc gggatatatc gacgcccgtc cgcctgatga cctactacag ctacaaagag
    28381 atcgcccgcc gcagccacac ggctgtcgag tgtcatctct cgcagtgctc gcgaaggtgc
    28441 gtctggcacc cacggagcaa gaaggtcgcg caaattacga tctgccacct gtcccctcgc
    28501 cttgattaat cacaaattca tttttatcgc ccgttgtcag cgcatccggc tcgatgttca
    28561 tggttcgcaa tacgccgccc atgatggcac caaagaccgg cgcggaaacg gcgccgccgt
    28621 agtatttacc cgcctgcgga tcgttgataa caacaaccag cgcgaagcgc ggctgactcg
    28681 caggcgcaac acctgcggtg taagcaatat atttattgat gtagcgaccg tccggcccga
    28741 cctttttcgc ggtaccggtt ttaatggcaa tacgatagcc tttaatcgcc gccttcacgc
    28801 cgccgccacc cggtagcgcc acgctttcca tcatatgcac cacagtgcgg acaatagatt
    28861 ccgggaagac acgttcaccg ggaaccgggg ggtcaacttt ggtaatcgac agtgggcgat
    28921 aaatgccgta gctgccgata gttgcgtaga ctcgcgctaa ctgtaacgga gttaccatta
    28981 gcccgtagcc gaaagagaag gtggccctct ctatgtcaga ccaccgttgt ttttgaggat
    29041 ataagccact gcgttctccg accaacccca aattggtcgc ttttcccagt ccaaaacgtg
    29101 agtaagtatc tactaacgct gaggacggca tcgctaacgc cagcttggaa acaccgacgt
    29161 tactcgactt ctgtaatact ccggtcaggg ttaattcgct gtagcgtgcc acgtctttga
    29221 tttcgtggcc gttaattcga taaggaatgg tattgagtac cgagttttcc cgcaccacgc
    29281 cacgttgcaa cgcggtcatc accaccatcg gtttaaccgt tgaacccggt tcaaacacgt
    29341 cggtgatggt acggttacgc atcgcctctt tcggcgtgcc gctcagattg ttagggttgt
    29401 atgacgggct gttagccatc gccagcactt caccggtgtt gacatccacc agcacggcgc
    29461 taccggactc agccttgtta aaggccaccg cgttgttcag ttcgcgataa accagcgcct
    29521 gcaggcgttc atcaatactc agcgccaggt tgtgcgcagc ctggctgtca gtagaagaaa
    29581 tatcttcaat tacgcgacca tagcggtctt tacgcacaat gcgctcaccc ggctgcccgg
    29641 taagccattt atcgaaactc ttctcaacgc cctcaatacc ttgactatcg acgttagtaa
    29701 agccgatgag gtgagcagtc acttcgccgg atggataata acggcgagat tcttcacgca
    29761 gatgaattcc cggcagtttc agttttttga tgtagtccgc catgtcaggg tttacctgac
    29821 gcgccagata aataaagcgc cctttcgggt tggcgttaat gcgggcagaa agctgatcca
    29881 gcggaatatt gagcgcgtta gccagcgcct tccagcggtc accgacgctg ataccgccag
    29941 cgtcatgcac ttctttcggg tcagcccaaa tcgcttttac cggcacgctc accgctaacg
    30001 ggcgaccaga acggtcagta atcatgccgc gggaggtgga aacttgctga acgcgaagag
    30061 aacgcatgtc gccctctttc accagcatat ccggggagat aacttgtaac cacgctacgc
    30121 gtccgagcag aaaagccagc gccaggagaa tacagccgca taacaacgca aaacgccaac
    30181 tgataaagtt ggcatgttct tcctgacgtt ttggtttctg cgttttcgcc gctgctttca
    30241 tgcgtcgcgt ttatccttat ttttgcacta cgatattttc ttgtgacgga tcaacatgct
    30301 gcatttgcag cttttccgtg gcgatccttt ccacccggct atggtcgccg agcgcattct
    30361 cttcaaggat caggttgcgc cattcaatgt ctaaagcatc tcgctccagc accagttgtt
    30421 cgcgctgagc ggtcagtaaa cgggtatggt gcgccgtggt taccacggtc accgccgtca
    30481 aaataatgca aatgaacagg cagagtggca gcttcccaaa tcgcaaaaga tcgtcaccga
    30541 taacaccagg caatgcatgg cgctcgtggc ttcccatcga tcctttaact ttgcttagag
    30601 cttctgtcac tctgctgatc atgcgttcgt cctctctgca atacgcagaa ctgaactacg
    30661 ggcacgagga ttctcagcca cctcttcttc gcccggcatt aatttgccta gtgctcgcag
    30721 ctgacggcca cccagttttt tgagctgctc ttcagtcatc ggtaaccctg ccggaacttg
    30781 cggaccgcgg ctgttttcac gcataaaacg tttcacaata cggtcttcca gcgagtggaa
    30841 gctgatgatc gaaagccgcc cacccggggc cagcacgttg agcgagcttt ttagcgcctg
    30901 ctctatctcc tccagttcac tgtttaccca aatgcgcacc gcctggaagg tacgggtcgc
    30961 gggatgttta aacttatctt tcaccggcgt tgcagcagcc acgacttccg ccagttcttt
    31021 ggtgcgggtc atcggctgtt cgcggttacg ctcgacaatg gctcgggcaa tgcgtttggc
    31081 aaaacgctct tcaccatagg ttttcaatac ccaggcgata tcggcttctt ctgcggtttg
    31141 tagccattca gcggctgact gcccacgggt tgggtccata cgcatgtcca gcggaccatc
    31201 gcgcataaag gaaaagccgc gttcagcatc atcaagttgc ggtgaagaga caccaagatc
    31261 gaggagaatg ccgtcgatct tgccgataag atcgcgctcg gcaacgtatt cgcccagcgc
    31321 ggaaaaaggt ccgtggatga tggagaagcg cggatcatca atagtcttcg caacggcgat
    31381 agcctgcggg tcgcgatcga tcgccagcaa acgcccctct tcgccaagct gcgagaggat
    31441 cagacgtgag tgaccaccgc gaccaaaagt cccatcaatg tagatgccat caggacggat
    31501 attgaggcca ttaacggctt catccagcag caccgtagta tgtttatagt tttccatcat
    31561 tttatagaga caagtcctgc agtcgctccg ataagtctcc ggtagccaac tgctctgcgt
    31621 cgatatcttc cttgacctgt tgatgccagg ttgtttcatc ccacagctca aacttgttga
    31681 actgtccaac cagcatcact tcttttgtca gcccggcatg ttgccgcagt actggcgcga
    31741 ttaacaatcg acctgcgcca tccatctgac attcgctggc atgacctaac agtaggcgct
    31801 gcacacggcg ctcaaccggg ttcatgctcg acagacgcga taatttttgc tcgataattt
    31861 cccattcagg cagggggtaa agcagcaggc acgggtgatg aatgtcaatg gtgcaaacca
    31921 tttgaccggc agcgttctca agcagctgtt cccgataacg ggtaggcact gataagcgcc
    31981 ctttgctgtc gagattgact aacgttgctc cccggaacat gccagcctca ccccttattc
    32041 ccactttgcc ccacaatttc ccactgaaag gagtttacgg agcggaggaa aagcttgtca
    32101 agccagtcac aaggcatacc gaggtaaaaa acccgatgtt tacggctaat atcagcgttg
    32161 tttattttac gtccaacgtg cgaagcaaat taacgttatg aatagctgta agaaaaaaac
    32221 cctcatcgtc atcaccgttt taaaatgact cactattttc cacagcaaat ataaagtgtc
    32281 agtttgcgac gcgagcggca ttttaggaca tatcttcccc ggttaacagt ccctgttgcg
    32341 tctgtgtagc gcttgatgcc agcgggtttg cgcaaaagca aagaaaagtg cttgttaaat
    32401 cggcaattca tctctgcatg taacaaaata atgcaaaaac gcgctgtcga tatcacttaa
    32461 ttattgtgaa tttcagctgg tgagttaatt aactggcggg tttaaaagca tttatcaata
    32521 atagtcagga atagtcttat ttactttaag gatattgatg tccagtcccg tactctacgc
    32581 gccagagtga aattcacctg gcgcgtattt ttgttcgcag cttagctacg gctgagcaca
    32641 ccgcggcgat agagattacg tttaatgcgc gttaaaccag gttttggctt acgcggttcg
    32701 tccaggcttg ccaggacaat ctccagcaca cgctctgcga catcgcggtg acgttgagcc
    32761 actgccagca ccgggcactg taagaagtcg agcagttcgt tatcgccaaa ggtggcaatt
    32821 gccaggtcag aaggcagttt accatcgcga cgtagcgtga catccatcac cccttgcaac
    32881 aacgcaaacg acgttgtgaa cagcgcctgc ggcatcggat gcgtttccag ccatttttcg
    32941 aataactggg cagccgcctc ccgctcatag ctgttggcat acaggaaatg cacttcgcgc
    33001 ggatcatctt tccaggcagt acggaaacct tgttcacgca ggaagctgac agaaagctcc
    33061 ggcagcgcgc caagataaag caccgtctcg gcgggaaact tacgtaactc ttccgccagc
    33121 atttcggcat catcctgatc ggcaccaacc acgctggtaa agtgttcacg atcgagggcg
    33181 cggtccagcg cgacaatcgg gaacgggtcg ttagcccagc gttgataaaa aggatgctca
    33241 ggaggcaacg acgtcgaaac aataatggca tcgacctgac gctgtaaaag gtgctcaatg
    33301 cagcgcattt cgttgtctgg ctgatcttct gagcaggcaa tcagcagttg ataaccccgt
    33361 tgccgcgcct ggcgttcaag atagttagcg atgcgggtat agctggtgtt ctccagatcg
    33421 gggatcacaa gaccaataga acgtgtgcgt ccagcacgaa gcccagctgc cacggcgttc
    33481 gggtggtaat tgtgctcacg caccacagcc atgacttttt caacggtttt gtcactcaca
    33541 cggtattgct tcgctttgcc gttaataaca tagcttgcag tggtccgcga cactcccgcc
    33601 agccgagcga tttcatccag tttcacaatt gccccttgcg taaaatgtaa aaaccatagc
    33661 cattgtactg gtatgggtta aattcattaa catctaagcg cagaaaatta actgcggcaa
    33721 ctgcttttat tccaggttcc cactgatttc gcaaaaaaag cccaacgtga catgttgggc
    33781 tgtaaattgc gcattgagat cattctcaac gcattatttt atcgccgcgc gaaagtccga
    33841 ccacaccaga gcgagcaact tccacaattt tcgccacatc gcgaatcgat gctaaaaatg
    33901 catcaagctt atcgctggtg cctgctaatt gaacggtata aagcgagggt gtgacatcaa
    33961 taatttgccc acggaatatt tccgtattac gtttaacttc gtcacgcccg taaccgctgg
    34021 cctgaatttt caccagcatg atttccctct caacatgcgc gccctgcccc aactcactca
    34081 cgcgcaagac atcgaccagc ttgtataatt gcttttcgat ctgctcaagc actttttcat
    34141 cgcccacggt ctggatggtc atacgcgata atgtcggatc gtcggttggc gcaacggtca
    34201 ggctttcaat gttgtagcca cgctgggaaa aaaggccaat cacgcgggat aacgcgcctg
    34261 attcattttc gagtaagact gataatatcc ggcgcataat caggttctct ccgttttgct
    34321 taaccacatt tcatccattc cgcccccgcg aatctgcatc gggtagacgt gctcactgcc
    34381 atcgacggtg acatcaacaa ataccaggcg attattacgc acctgttcaa gcgcctcgct
    34441 aagtttgctt tccagctctt gcggatgaga gatctggatc ccgacgtgac cataggcttc
    34501 cgccagacgg acgaagtctg gtaaagactg catataagat tgtgaatgac gaccggaata
    34561 gatcatgtcc tgccactgct tcaccatccc caaatagcgg ttattaagat taaccaccag
    34621 caccggcagt tcatattgca acgctgtaga cagttcctga atgttcatct gaatactgcc
    34681 gtcgccagtg acgcaaacca cggtttcttc tggcagcgcc attttgacgc ccagtgccgc
    34741 aggtaaacca aagcccatag tgccgaggcc accggagttg atccaacgac ggggtttgtc
    34801 gaatggataa taaagcgcag caaacatctg gtgctgcccg acatcggacg taacgtaagc
    34861 gccccccttc gtcaaccgcc aaagagtctc gatcaccgcc tgcggtttaa tcttttcact
    34921 gtgagtgtca tatttcaggc actgacgagc gcgccactgt tcaatctgct gccaccagtc
    34981 gcggatctca tccagcggtt gatgggcgga ttcttgcgac aagagttcaa gcatttgttc
    35041 gaggacctgg cgagcatccc ccacaatcgg aatatccgca gtcacggttt tagaaatgga
    35101 agtaggatca atatcgatat gcagaacagt ggcatttggg cagtactttg ccagattgtt
    35161 agtggtgcga tcgtcaaagc ggacaccgac agcaaaaatc acatccgcgt tatgcatcgt
    35221 catattggct tcgtaggtac cgtgcattcc cagcatgccc agtgcctgac gatgcgttgc
    35281 cggaaacgct cccagcccca tcaatgagga aacaacgggc agatttaacg cctccaccgt
    35341 ttctttcagc tgttgatggc agcccgccgt gattgcccca ccgcctacgt agacaaccgg
    35401 tttttttgcc gccaccagcg tttgcagagc gcgcttaatt tgccctttat gtccggtagt
    35461 agtgggattg taagaacgca tactgaccga ctccggccag acatagggta atttgttcgc
    35521 cggattaaga atatctttcg gtaaatcaac gaccactggt cctgggcgac cacttgccgc
    35581 cagccagaaa gcctttttca gcacctgcgg aatgtcttcc gtttgcttaa ccagaaaact
    35641 gtgtttaacc accggtcgcg aaatccccac catgtcgcac tcctgaaagg catcgtaacc
    35701 tatcaacgag gtcgctacct gcccggaaag gacaactaat ggaatggaat ccatataagc
    35761 ggtggcgatg ccggtaatcg cattggtcgc ccctggaccc gacgttacca gcacgacgcc
    35821 gacttccccg gtcgcgcgcg ccagaccatc ggccatatgc actgccgcct gctcatgacg
    35881 aactaaaaca tgaccgatac cacccacggt atgcaatgca tcgtaaatat caaggactgc
    35941 gcctccggga taaccgaata cttgtttaac gccctgatcg ataagcgatc ggacgaccat
    36001 ctcggctcca gacaacatct ccatggcctg cctcactgtt tgacggaaaa aatgtgtaaa
    36061 acacattaac cgtccgatct tgcttaagca attggcagcc agagacataa acgccgggta
    36121 ataagaataa acaggaggaa aggtgaaaag aaacgataaa atcctccatt acgaaggata
    36181 aaaatgataa taaatcggct aaatcccacc acttacagca atttctctat ttttttaatt
    36241 aatcctaaaa tagagagccc acacattcag aataaaattc tacactcaat aaaaaatgca
    36301 gaataaacca gacattcatg tctgacctat tctgcattca gttagcgttt gcaaattgag
    36361 actaattgct cttccatcca ctgatggcct ttatcacgcc cggcagcttc atgccaggag
    36421 agataacagg ttctgctatt tagttttaac ggcagcggta atacctgtaa ttctaaggat
    36481 tcagcgaact cttcagccag ccaacgcggc gcaatagcga ccaaatgcgt ttgcgacacc
    36541 acgctaagta cgctcatcat tgccatgccc tgatacgcga tactggcttg cttatctacc
    36601 gtgtcatacc aaggttgact aaatgacgcg aaacgatcga gcgaaaccgc cgcatgttgt
    36661 tcgttataaa catcatgttt cagtaacggg cccttaattg ttgggtgatt tttgctggct
    36721 accagcacca tttcatcttt aaataatggc acgctggtaa attcaggacg atggaagtct
    36781 tcataactaa tcacaaactc cgtttcctga taacgcagct gatgttcagt gttctgattt
    36841 aatgaagact tgaacataac atgtatattt ggcgcaatct gctcaatgtg attataaatc
    36901 tgcgaggtca gaatactgtc taacgggctg caaacacaaa gatgaaatac acgttcactg
    36961 ctcgcgggtt caaaacctga accaggcaat tcattttgta ctagttgcaa tgcctgacga
    37021 actgaaccaa aaagttgaaa tgcgcgagca gtcggttgaa taccacggcc ataacgaaca
    37081 aaaagctcgt cattaaacat caccttcagg cgtgcaacag cgttactgac cgcaggttgc
    37141 gacattccca gaacatgagc agcacgggta atgttttgct cctgcatcac ggcatcgaaa
    37201 acggttaata agttgagatc gaccatgcgt agctgtggct tgctgaactc cgccgtctct
    37261 ggatgatctg tttgtacctc tggcatactt aactccactg tcacacttaa ctccctttcc
    37321 cttattggaa tgcagagaaa taatcctcga gaatatgatt tatcatgcat ataaaataag
    37381 aaaaaggaaa atgagtaaaa ttaggaaaac ataaagatac
//
LOCUS       DAZKIP010000034        37144 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZKIP010000034 DAZKIP010000000
VERSION     DAZKIP010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37144
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.034"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(67..486)
                     /locus_tag="ACHOUK_001961"
                     /pseudo
     CDS             complement(67..486)
                     /locus_tag="ACHOUK_001961"
                     /inference="COORDINATES: protein motif:HMM:NF025085.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(480..623)
                     /locus_tag="ACHOUK_001962"
     CDS             complement(480..623)
                     /locus_tag="ACHOUK_001962"
                     /inference="COORDINATES: protein motif:HMM:NF025085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9897424.1"
                     /translation="MNAFKQLHQSKDEVFERGVINVFRGPNGITKPIPPVNSAVKLSS
                     TTW"
     gene            complement(684..1025)
                     /locus_tag="ACHOUK_001963"
     CDS             complement(684..1025)
                     /locus_tag="ACHOUK_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9897425.1"
                     /translation="MSGIIISRPEVDTGHTDVICSTSIRHVVTVRNAALQQTETLIRQ
                     LAEISVLTAADIGGKTALDRAMKQDFHCGCWLMEKPETAMKAITRNLDCEIWRDLMQR
                     SGMLSLMDAQG"
     gene            complement(1110..1307)
                     /locus_tag="ACHOUK_001964"
     CDS             complement(1110..1307)
                     /locus_tag="ACHOUK_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFP9897426.1"
                     /translation="MTSLTPEAALDILIAWLQDNIDSESGIIFDNDEDKTDSAALLPC
                     IEQVREDVRTLRQLQLLQQNR"
     gene            complement(1319..1810)
                     /locus_tag="ACHOUK_001965"
     CDS             complement(1319..1810)
                     /locus_tag="ACHOUK_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312567.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFP9897427.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDIDGVNLAELINMVCDNGY
                     SLRVVDESDRTSADCTPPFTALTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGNGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAGCLPGLPT
                     FNW"
     gene            complement(1807..2181)
                     /locus_tag="ACHOUK_001966"
     CDS             complement(1807..2181)
                     /locus_tag="ACHOUK_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFP9897428.1"
                     /translation="MKTLPDTHVREASRCPSPITIWQTLLGRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEAK"
     gene            complement(2271..2423)
                     /locus_tag="ACHOUK_001967"
                     /pseudo
     CDS             complement(2271..2423)
                     /locus_tag="ACHOUK_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0051495 - positive regulation of
                     cytoskeleton organization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
     gene            complement(2432..3648)
                     /locus_tag="ACHOUK_001968"
     CDS             complement(join(2432..3340,3343..3648))
                     /locus_tag="ACHOUK_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611429.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEc31 family transposase"
                     /protein_id="HFP9897429.1"
                     /translation="MISSPQHKTGDLMNKKTKRTFTPEFRLECAQLIVDKGYSYRQAS
                     EAMNVGSTTLESWVRQLRRERQGIAPSATPITPDQQRIRELEKQVRRLEEHNTILKKA
                     NRALDVRLAERFTIVARLSDSHSVVSLCSALEIHRSSYQYWRKRRDTVNPARVRLCSE
                     IRRAWNQSRGSAGARTLAEMLTQNGVPMSRYRAGRLMKYLNLSSCQPGKHQYKNARQE
                     HTCLPNLLERQFAVPEPDRVWCGDITYIWAGNRWCYLAVVMDLFARRVIGWSLSANAD
                     TALISSALRMAYEVRGQPRDVMFHSDQGSQYTGLKYQQLLWRYRIKQSVSRRGNCWDN
                     SPMERFFRSLKTEWVPTDGYTGKDVARQQISSYILNYYNSVRPHHYNGGLTPEESENR
                     YHFYCKTVASIT"
     gene            complement(3747..4928)
                     /locus_tag="ACHOUK_001969"
     CDS             complement(3747..4928)
                     /locus_tag="ACHOUK_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9897430.1"
                     /translation="MALTDVKVKTAKPKERPYKLADGGGMYLLINANGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKILAAGGDPGEAKKEEKIALQMSLKNTFEA
                     VAREWHQTKADRWSLRYRDEIIDTFEKDIFPYIGKLPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLTD
                     LAGYTGSIITKTATQIIMLTGVRTQELRFAHWEDIDFEAKLWEIPAEVMKMKRPHIVP
                     PSEQVIALFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHAQYLDGRREMMQWYADYIDSLSEL
                     A"
     gene            complement(5124..5218)
                     /locus_tag="ACHOUK_001970"
     tRNA            complement(5124..5218)
                     /locus_tag="ACHOUK_001970"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5182..5184),aa:Sec,seq:tca)
     gene            5511..6893
                     /gene="yicJ"
                     /locus_tag="ACHOUK_001971"
     CDS             5511..6893
                     /gene="yicJ"
                     /locus_tag="ACHOUK_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFP9897431.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDNFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLCGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            6903..9221
                     /gene="yicI"
                     /locus_tag="ACHOUK_001972"
     CDS             6903..9221
                     /gene="yicI"
                     /locus_tag="ACHOUK_001972"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFP9897432.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAGRNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTALAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFSEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPVYVRDNTLLALGNNEQRPDYAW
                     HEGTAFQLFNLQDGHEAICEVPAADGSVLFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     IVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(9274..10983)
                     /gene="yicH"
                     /locus_tag="ACHOUK_001973"
     CDS             complement(9274..10983)
                     /gene="yicH"
                     /locus_tag="ACHOUK_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFP9897433.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTTLDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSSEVTSTE
                     PVQ"
     gene            complement(11104..12495)
                     /gene="xanP"
                     /locus_tag="ACHOUK_001974"
     CDS             complement(11104..12495)
                     /gene="xanP"
                     /locus_tag="ACHOUK_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFP9897434.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            12775..13980
                     /gene="gltS"
                     /locus_tag="ACHOUK_001975"
     CDS             12775..13980
                     /gene="gltS"
                     /locus_tag="ACHOUK_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFP9897435.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            13983..14849
                     /locus_tag="ACHOUK_001976"
     CDS             13983..14849
                     /locus_tag="ACHOUK_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9897436.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISEWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIRSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(14834..16915)
                     /gene="recG"
                     /locus_tag="ACHOUK_001977"
     CDS             complement(14834..16915)
                     /gene="recG"
                     /locus_tag="ACHOUK_001977"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFP9897437.1"
                     /translation="MTGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(16921..17610)
                     /gene="trmH"
                     /locus_tag="ACHOUK_001978"
     CDS             complement(16921..17610)
                     /gene="trmH"
                     /locus_tag="ACHOUK_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFP9897438.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(17617..19725)
                     /gene="spoT"
                     /locus_tag="ACHOUK_001979"
     CDS             complement(17617..19725)
                     /gene="spoT"
                     /locus_tag="ACHOUK_001979"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFP9897439.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(19744..20019)
                     /gene="rpoZ"
                     /locus_tag="ACHOUK_001980"
     CDS             complement(19744..20019)
                     /gene="rpoZ"
                     /locus_tag="ACHOUK_001980"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFP9897440.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(20074..20697)
                     /gene="gmk"
                     /locus_tag="ACHOUK_001981"
     CDS             complement(20074..20697)
                     /gene="gmk"
                     /locus_tag="ACHOUK_001981"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFP9897441.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRDQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            20955..22637
                     /gene="ligB"
                     /locus_tag="ACHOUK_001982"
     CDS             20955..22637
                     /gene="ligB"
                     /locus_tag="ACHOUK_001982"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFP9897442.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEIFRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAKVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHRFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(22634..23251)
                     /gene="yicG"
                     /locus_tag="ACHOUK_001983"
     CDS             complement(22634..23251)
                     /gene="yicG"
                     /locus_tag="ACHOUK_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFP9897443.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(23543..24367)
                     /gene="dinD"
                     /locus_tag="ACHOUK_001984"
     CDS             complement(23543..24367)
                     /gene="dinD"
                     /locus_tag="ACHOUK_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFP9897444.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAAVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            complement(24588..25451)
                     /gene="yicC"
                     /locus_tag="ACHOUK_001985"
     CDS             complement(24588..25451)
                     /gene="yicC"
                     /locus_tag="ACHOUK_001985"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFP9897445.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            25578..26294
                     /gene="rph"
                     /locus_tag="ACHOUK_001986"
     CDS             25578..26294
                     /gene="rph"
                     /locus_tag="ACHOUK_001986"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFP9897446.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            26360..27001
                     /gene="pyrE"
                     /locus_tag="ACHOUK_001987"
     CDS             26360..27001
                     /gene="pyrE"
                     /locus_tag="ACHOUK_001987"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFP9897447.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(27038..27634)
                     /gene="slmA"
                     /locus_tag="ACHOUK_001988"
     CDS             complement(27038..27634)
                     /gene="slmA"
                     /locus_tag="ACHOUK_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFP9897448.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(27741..28199)
                     /gene="dut"
                     /locus_tag="ACHOUK_001989"
     CDS             complement(27741..28199)
                     /gene="dut"
                     /locus_tag="ACHOUK_001989"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFP9897449.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(28177..29397)
                     /gene="coaBC"
                     /locus_tag="ACHOUK_001990"
     CDS             complement(28177..29397)
                     /gene="coaBC"
                     /locus_tag="ACHOUK_001990"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFP9897450.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            29569..30237
                     /gene="radC"
                     /locus_tag="ACHOUK_001991"
     CDS             29569..30237
                     /gene="radC"
                     /locus_tag="ACHOUK_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFP9897451.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            30454..30690
                     /gene="rpmB"
                     /locus_tag="ACHOUK_001992"
     CDS             30454..30690
                     /gene="rpmB"
                     /locus_tag="ACHOUK_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFP9897452.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            30711..30878
                     /gene="rpmG"
                     /locus_tag="ACHOUK_001993"
     CDS             30711..30878
                     /gene="rpmG"
                     /locus_tag="ACHOUK_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFP9897453.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            30976..31785
                     /gene="mutM"
                     /locus_tag="ACHOUK_001994"
     CDS             30976..31785
                     /gene="mutM"
                     /locus_tag="ACHOUK_001994"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFP9897454.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(31824..32303)
                     /gene="coaD"
                     /locus_tag="ACHOUK_001995"
     CDS             complement(31824..32303)
                     /gene="coaD"
                     /locus_tag="ACHOUK_001995"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFP9897455.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(32311..33588)
                     /gene="waaA"
                     /locus_tag="ACHOUK_001996"
     CDS             complement(32311..33588)
                     /gene="waaA"
                     /locus_tag="ACHOUK_001996"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFP9897456.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            34001..35059
                     /gene="rfaQ"
                     /locus_tag="ACHOUK_001997"
     CDS             34001..35059
                     /gene="rfaQ"
                     /locus_tag="ACHOUK_001997"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFP9897457.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            35056..36180
                     /gene="waaG"
                     /locus_tag="ACHOUK_001998"
     CDS             35056..36180
                     /gene="waaG"
                     /locus_tag="ACHOUK_001998"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP9897458.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            36173..36970
                     /gene="rfaP"
                     /locus_tag="ACHOUK_001999"
     CDS             36173..36970
                     /gene="rfaP"
                     /locus_tag="ACHOUK_001999"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFP9897459.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVHDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT         8739 a         9216 c         9856 g         9333 t
ORIGIN      
        1 tttgttgacg tcgggagagt tcagaagagg aagtttggcg gagatcacag gagtcgaata
       61 tgattattaa tgcattgatt ttaaatgatt aatgcgtgtt ggataaatga ttgaccccca
      121 taataacccc caaatgactt tacatgaggg aatgaggcta aaatacacaa ttgaatcaat
      181 tcttagagat ttttgtgaag gtaacattcc taggtagtgt ctggcaataa tatcattaag
      241 cctgtcaacc agctctggct ttctgaatgt gatgtgtgcg ggcccttctt aaagtattta
      301 cttgtcttca tagcgctctt taccctgaac ggactgtagg tggtcatcca gattaatggt
      361 gatattccct cggttgttgg ggagcggttt accgctgagc agatgcaaca ttctttccag
      421 gtcagctaag cgatctgcct gcattccact gataagatga aatctccatc tgtcccatct
      481 caccaagttg ttgacgataa ttttactgcc gaatttacag ggggaattgg ttttgtaatt
      541 ccgttcggcc ctctgaagac gttgatcacc ccacgctcaa acacttcatc cttactctgg
      601 tgcagttgtt tgaatgcatt catgatattc gcctcgctga tttccggaat attatcgtac
      661 tccagtgagc ggtaccatgt atcctagccc tgtgcatcca tcagactcag catcccggag
      721 cgttgcatca ggtcacgcca gatttcgcag tcgaggttgc gggtgatggc tttcattgcg
      781 gtttcaggtt tctccatcag ccagcagccg cagtgaaaat cctgtttcat cgcccggtcc
      841 agggcggttt tgccgccaat gtctgctgct gtcaggactg agatttctgc cagttgccgg
      901 ataagcgttt cggtttgttg cagtgctgca ttccgtaccg tcacaacgtg tcggatactg
      961 gtggagcaga taacgtccgt atgcccggtg tccacttccg gacgggagat tatgatgcct
     1021 gacattatcg gttttccttt tggtttataa acgaaaacgc cagccctgtt caggctggcg
     1081 tcagtggaat gaagtatggt gagtgagact caccggttct gttgcagaag ctgcagttgg
     1141 cgcagggtac ggacatcttc cctgacctgt tcgatacagg gcaacaatgc tgccgaatcc
     1201 gttttatcct catcgttgtc gaagataatt ccggattcgc tgtcgatatt gtcctgcagc
     1261 cacgcaatca gaatatccag tgctgcttcc ggggttaatg atgtcatgtt gttcctagtt
     1321 accaattaaa agtgggtaaa cctggcagac atccggcact ggcatccaga tgaatgagac
     1381 tgacaccata acgccggatg agtgtgacga ccagacgacg gaacgttttt gacagaccca
     1441 ggcgtttaag ccgcagaacc gggtacgacc acgcatcggt acgcaacaga tagccgttac
     1501 cggtaaaatg aatccattct gattcaccga agtcactggt ctggtgtgac agcgagtaca
     1561 gccaggcgtt gtccgtttcc gtgatatgtg cggtactgca gcgtatgccg gtaagggcag
     1621 taaatggtgg cgtgcagtct gctgaggtcc ggtcagattc atcaacaaca cgaagggagt
     1681 agccgttatc gcagaccatg ttaatcagtt cagcgagatt aacaccatca atgtcaacga
     1741 caattcgccc catattcagt gcctgtacgt taacgctgtc ggcttccagt gtcagggcca
     1801 gtttcatcat ttcgcctccg gatgcttacc cagggtaatg ttatttaccg ttctgtaatt
     1861 gtcgcgggtc atcaggccgg tcgccctgcg agcccggagg atatcgatgc tgtttattaa
     1921 ctgagagcgg gtacaggcgc tgaatcccgg ctggtcggta cgcaccagca cgtatttttc
     1981 cacgagaaag ttcaccgcat cacacagtga aatacctgcc tcaatatgct gctcaatcac
     2041 acgttcatca gcgaacggtg tgtcattcag cgtgaggccg tagtgctggt ccagcagacg
     2101 gccaagcagt gtctgccaga tggtgatggg agacgggcag cgcgatgcct cccgtacgtg
     2161 tgtatcgggt aatgttttca tactaaagtt tttcctgata tgcagagatg agaaatgggg
     2221 aagtggcgtg gtgaaaacac caggccggag cagaaggtta ttctgatgat ttactttttc
     2281 gtttcgggcg tcggataaac agccatataa acgtaacctc gactgccgag ggtatcggct
     2341 ttgcaggtca gcccttttgc atacagcgtg acggtatgct gatggcgggg attcagctca
     2401 ccgctggtga gcatgagttc cagtgtagtg gtcaagtaat actggccacg gttttacagt
     2461 aaaaatgata tctgttctct gactcttccg gcgtcagccc tccgttataa tggtgaggcc
     2521 tgacgctatt gtaataattc agaatataac tgctgatttg ctgccgggcc acgtctttgc
     2581 ctgtgtagcc atcggttggc acccattctg ttttcagact gcggaagaag cgttccattg
     2641 gactgttatc ccagcagttt ccccgtcggc tgacactttg ctttatcctg taacgccaga
     2701 gaagttgttg atatttcagt cctgtatatt gacttccctg gtcgctatgg aacatgacgt
     2761 cccgcggctg accacgcacc tcatacgcca tccgcagggc actgcttatc agggcagtat
     2821 cggcattcgc tgacaggctc cagccgataa ccctgcgggc aaaaagatcc atgacgaccg
     2881 ccagatagca ccagcgattt cctgcccaga tatacgtaat atctccgcac cataccctat
     2941 ctggctcggg cacagcgaac tggcgctcaa gcagattcgg caggcaggta tgttcctgac
     3001 gagcattttt gtactgatgt tttccgggct gacaactgct caggttcaga tatttcatca
     3061 gacgcccggc acggtaacgg ctcatcggga cgccgttttg ggtcagcatt tcagccagcg
     3121 tgcgcgcccc cgcagagccc ctactttggt tccacgcccg gcgtatttcg ctgcacaacc
     3181 tgactcgcgc cggattaacc gtatcgcgtc gttttcgcca gtactggtaa ctgctgcggt
     3241 gtatttccag agcagaacag aggctgacaa ctgagtggct gtcactcagt ctggcaacta
     3301 tcgtgaaccg ttcagcgagt cggacatcaa gagcgcggtt gtagcctttt ttaatatcgt
     3361 attgtgttcc tccaggcggc gaacctgctt ttccagttcg cggatacgtt gctggtctgg
     3421 agtaataggt gtggcagagg gcgcaatccc ctgacgctct cgcctgagct ggcgcaccca
     3481 actctcaagc gtggtagaac cgacattcat cgcttcactg gcttgtcgat atgagtagcc
     3541 cttatcaaca attagctgtg cacattccag cctgaattca ggggtgaaag tacgtttggt
     3601 tttcttgttc attaagtcac ctgttttgtg ttgtggtgag gatatcacct ttaatcaggt
     3661 ggccaaattt actgtgccac tacaaccatc cgcctgttgg ggctggatgg ttattcatta
     3721 tttttgtgtc accgaacttt cattcatcag gccagctcag aaagtgaatc aatgtagtca
     3781 gcgtaccatt gcatcatttc acggcgacca tcgagatact gggcgtggtt ataggtaccc
     3841 ctgatggagt ttttatccac atgagccaac tgcatttcaa tccaggcaga attaaagcct
     3901 tgttcgtgca gaattgtgct catggtatgc ctgaaaccgt gccctgtgag tcttccctta
     3961 taccccagca attcaatgac ctggttaatg ctctccttac taattggctt gcgaggatcg
     4021 ttcctaccga taaagaccag aggatgatgt tttgagattg gttcaagttg cttaaatagc
     4081 gcaataacct gctcagaggg cggcacaata tgaggccgtt tcatcttcat tacttctgcc
     4141 gggatctccc ataattttgc ctcaaaatca atatcctccc aatgcgcgaa acgcaactct
     4201 tgcgttcgta caccggttaa cataatgatc tgagtagctg tcttagtgat gatacttccg
     4261 gtataacccg ccagatcagt aaggaagtgg ggaagttcat tggcagtaag aaagggaaaa
     4321 tgtacttttt tcggtgtagc taaagcactg gcaagatcgg gagcagggtt atagtcggct
     4381 cgaccagtaa caattgcata gcgaaacacc tcaccacaac gttggcggac cttgcgcatt
     4441 ttctccagtg ctccacgttt ctccatctta cgtagtgctt ccaatagctc catcggcttg
     4501 atttcggcaa taggtagctt gccgatgtag gggaagatat ctttctcaaa ggtgtcgatt
     4561 atttcatcgc ggtagcgcag tgaccagcga tcggctttcg tctgatgcca ctcgcgagca
     4621 acggcctcga aagtgttttt caaactcatt tgcagcgcta ttttttcttc tttttttgcc
     4681 tcacccggat cgccaccagc cgcaagtatt ttcctggctt cactgcgttt ctcacgtgcg
     4741 tcggcaagcg ttacatcggg gtatacgcca atagacagca tcttttcttt accggcgaag
     4801 cggtacttca tacgccagta tttggaacca ttagcgttaa tcagcagata catacctccg
     4861 ccatcagcca gcttataggg cctttctttt ggcttcgcgg ttttcacttt aacatcggtt
     4921 agcgccatga catcctccag cttttgatga atccatcctc ctgggggaat ttccgccagt
     4981 aaccaacctg atacccccaa tgcaccccca caaacaactt gatatgggtt aaagtcgttt
     5041 gacctcagga tacaaagaaa acctgatata taagggatta taaggggctt tgttgatgtc
     5101 gggagagttc aggagaggca aactggcgga agatcacagg agtcgaacct gcccgggacc
     5161 gctggcggcc ccaactggat ttgaagtcca gccgcctcac cggagacgac gatcttccgc
     5221 gcctcgattg ctacatggag gcggggcgca ttatagctac ttccttgagt ttctacatcc
     5281 cccagatcag ttttttccgc ctaatacacg cagcgtatta tcacgaaagc attataattc
     5341 atataaaact catattttac aatgatatat gagcggaatt aacgctacca cgatcacata
     5401 aaacaagaat cataaattaa taaccagata tcggaatatt cgctctcaca gggatggttt
     5461 acaaaatgcg ttatcggtct cagcacccct atagcatcaa ggaaaagcag atgaagagtg
     5521 aagtgttgtc cgttaaagag aaaattggtt atggcatggg agacgccgcc agccacatta
     5581 ttttcgataa cgtaatgtta tatatgatgt tcttttatac cgataatttt ggcatacctg
     5641 ccggatttgt cggaaccatg tttttggtcg ctcgtgcact ggatgcgatt tccgatcctt
     5701 gcatggggtt gttggccgat cgaacgcgct ctcgctgggg taaatttcgt ccgtgggtac
     5761 tgtgtggcgc actgccattt gggatcgtct gtgtactggc ctatagcacg ccagatctca
     5821 gtatgaacgg caaaatgatc tatgcagcaa ttacttacac cctacttacc ttactttata
     5881 ccgtcgtcaa tatcccttac tgcgcattgg gtggtgtaat caccaatgac ccgactcagc
     5941 gtatctcgct gcaatcctgg cgttttgtgc tggcgaccgc gggaggcatg ctctctactg
     6001 ttctgatgat gccactggtt aatttaattg gcggtgataa taaaccactc ggtttccagg
     6061 gcggtatcgc ggtcctttcc gtggtggcat tcatgatgct ggcattctgt ttcttcacca
     6121 ctaaagaacg cgttgaagca ccacctacaa cgacgtctat gcgggaagat ttacgtgata
     6181 tctggcaaaa cgaccagtgg cggattgtcg gtttactaac cattttcaat atcctggcgg
     6241 tgtgcgtccg cggtggagcg atgatgtatt acgtcacatg gattttgggt acgccggaag
     6301 tgtttgtcgc ttttctcacc acttattgcg tgggtaacct gattggttcc gcactggcaa
     6361 aaccgctgac cgactggaaa tgtaaagtca ctatcttctg gtggacgaac gccctgctgg
     6421 cagtgattag cctcgcgatg ttctttgttc ctatgcaggc cagcatcact atgtttgtct
     6481 tcatcttcgt gattggtgta ttgcatcaac tggtgacacc tatccagtgg gtaatgatgt
     6541 ccgataccgt cgactacggc gagtggtgca atggtaaacg cctgaccggg atcagttttg
     6601 ctggcacgct gtttgtgctc aaactggggt tggccttcgg cggcgctctt atcggctgga
     6661 tgctggctta tggcggatat gatgcggcag aaaaagcgca gaacagcgcc acgattagca
     6721 tcattattgc gctattcacg attgttccgg cgatctgtta tttgctgagc gcgattatcg
     6781 ctaaacgcta ctactcactc acgacgcaca atctgaaaac cgttatggaa cagctggccc
     6841 agggcaaacg ccgttgccag caacaattca cctctcaaga agtgcagaac taaggaacgg
     6901 caatgaaaat tagcgacgga aactggttga ttcaacctgg cctcaatttg attcacccgc
     6961 ttcaggtgtt cgaggttgaa cagcagggta atgaaatggt ggtctatgct gccccccgtg
     7021 atgtgcgtga acgaacctgg cagcttgata cgcctttatt tacgctgcgc tttttctccc
     7081 cacaggaagg tattgtcggt gtacggattg agcattttca gggggcgctg aataacggtc
     7141 ctcattatcc gctcaatatt ttgcaggacg tgaaggtcac aatcgaaaac acagaacgtt
     7201 acgctgagtt taaaagtggc aacttaagcg cgcgtgtcag caaaggtgag ttctggtcac
     7261 tggattttct gcgcaacggc gaacgtatta ccggtagtca ggtgaaaaat aatggctacg
     7321 tgcaggacac gaataatcaa cgaaattata tgtttgagcg gctggatctt ggcgttggcg
     7381 aaacagttta cggtctggga gagcgcttta ctgccctggt gcgcaatggc cagacggtag
     7441 agacctggaa ccgggacggc ggcacaagta ctgaacaggc gtataaaaat atcccgttct
     7501 atatgactaa ccgtggctat ggggtactgg tcaatcatcc tcaatgcgtc tcttttgaag
     7561 tgggatcgga gaaagtctcc aaagtgcagt tcagcgttga gagtgaatat ctcgaatact
     7621 ttgttatcga cggcccgacg ccgaaagcgg tacttgatcg ttatacccgt tttactggtc
     7681 gtccggcgct gccgcccgcg tggtccttcg gcctgtggct aaccacttca tttaccacca
     7741 actacgacga agcgacggta aacagcttta tcgatggtat ggcgggacgc aatctgccac
     7801 tgcatgtttt tcactttgac tgtttctgga tgaaagcctt ccagtggtgc gattttgagt
     7861 gggacccgct gactttcccg gacccggaag ggatgatccg ccgcctgaaa gcgaaagggc
     7921 taaaaatctg cgtctggatt aacccctata tcgggcaaaa atcccctgtc tttaaagagt
     7981 tacaagagaa aggctattta ctcaaacgcc cggacggttc gttatggcag tgggataaat
     8041 ggcagccagg tctggcgatt tatgacttta ccaatccgga tgcctgcaaa tggtacgccg
     8101 acaaactgaa aggtctggtc gcgatgggcg ttgattgctt taagaccgac tttggcgaac
     8161 gtattccaat cgatgttcag tggtttgacg gttccgatcc gcagaaaatg cataaccatt
     8221 atgcgtacat ctacaacgaa ctggtgtgga acgtgctcaa ggacaccgtt ggcgaggaag
     8281 aagccgtctt gtttgctcgc tcggcctccg ttggtgcgca gaaattcccg gtacactggg
     8341 gtggtgactg ttacgctaac tacgaatcaa tggcggaaag cctgcgcggt ggtttgtcta
     8401 ttggcctttc aggttttggt ttctggagcc acgatatcgg cggctttgag aataccgctc
     8461 tggcgcacgt ttacaaacgc tggtgcgcgt ttggtttgct ctccagccat agccgtttac
     8521 atggcagcaa atcttatcgt gtgccgtggg cctacgatga tgagtcctgt gatgtggtgc
     8581 gcttcttcac gcaactgaaa tgccgcatga tgccgtatct gtatcgtgaa gctgctcgtg
     8641 cgaacgcgcg gggtacgccg atgatgcggg ccatgatgat ggagttcccg gacgatccgg
     8701 cttgtgatta ccttgaccgt caatacatgt taggcgacaa cgtgatggtt gctccggtgt
     8761 tcagtgaagc gggcgatgtg cagttctact tgccggaagg tcgctggaca catctgtggc
     8821 acaacgatga actcgatggt agtcgctggc ataaacagca gcacagcttc ctgagtctgc
     8881 ccgtttatgt gcgtgataac accctactgg cgctgggcaa caacgagcaa cgtcccgatt
     8941 acgcgtggca cgaaggcacg gcattccagc tctttaatct acaagacggg catgaagcca
     9001 tctgtgaagt gcccgctgct gacggttccg ttcttttcac cctgaaagcg gcgcgtactg
     9061 gcaacacgat tactgtgact ggtgcgggcg aggcgaagaa ctggacactg tgcttgcgca
     9121 atattgtgaa agtaaatggt ctgcaaggtg gttcgcaggc tgaaagtgag caggggctgg
     9181 tggtgacgcc tcaagggaat gcgctgacaa ttacgttgta atctattgat ggaatgccgg
     9241 gtacgtctga gtcgtacccg gcgagattga tttttactgc accggctctg tcgatgtaac
     9301 ttcgctactg gaaacgacgc ccgcattcat ggtttgagtc acttgctgct ttgcggcgtt
     9361 cacggcatgg agttgcccgc taaccgtagg tttcagcggg acattggcct gaatatcgcc
     9421 actggcggta agctgaatat tgccgtcgcc agtcaacggt aattcaggcc atccccattg
     9481 ttgcaaaata ttcaccggca caccgcgtcc attcaggctg atatgggtct gacgttgtgg
     9541 cgtttgtgaa acactggcag tggcttccag aatgcctttt tcagtaaatg cactcagttc
     9601 gctgatattc accgtgctgc tgttggcggt cagcgccagc gacgggcgac gaacatcaac
     9661 acgattgaat gtcgcggcgg cggcattcag attcgccgag ccactccaga cgccccattt
     9721 atgatcggta accagcgtca ggttggcacc gtaaccatcg agcgtggtga gctgccacgg
     9781 gaagtcaggg tcgatatcaa tgatcagatt gcggctggcg ctaaatctct tcagttgcag
     9841 gctgtttaac caaccgggtg tcgtttccat ccacaactgt tgccagtttt tcggcaaggt
     9901 atattccagc ccggcaattg ccgcatcatc aaggatcaac gttttcccgt cacgcagcca
     9961 gttccctgac gttctgacca tacccccttc ccagcggctg gtgaactggc gcagcgctac
    10021 gccctgcggg gaaaattccg cgtttataat cgggtcaaat aaatgcagcg aaccataaat
    10081 gaactcgcta gcgttcatcg acagtttgcc ttcttgtgtc tgccagtcat ctttactgaa
    10141 ggtcatgttg cgcaagctga gatcgaggtc ggtcaccgcc cagtccggac cttgcaaacg
    10201 agcatcgatc acttccaggc gaccaatctg caacgacggg acagagcgta atggcgcaaa
    10261 gaagtcggtt agcgattttt cgctttgtag acggatatcc gccatgcgca gattttccac
    10321 ttgccagctg ccgtcggcgt tacgctgcgc gtttccggtt aatgtcccgc gggcgatgtc
    10381 ggcacccagg ttagtcagcg taacgcgatc gttatcaata ctgccttcaa tcagtacatt
    10441 ggtggcagga acatcattga gcgaaagcga tccggcacta aactgaatct gcgccttcgt
    10501 acccagcact ttaccggctt ctggtgacca cggaaccacg ccgccattta cccgctgcgc
    10561 gctcagtttc cattcgctat tcgggctatt aaacgccata tcacgcagtt gcagacgatc
    10621 ggctttgaac ggtagcggcg cggtctggtc ggtgagattc agcgtcccgt tttccagcag
    10681 gatggtatcg acatggcgtg gttcggtcag ttgccgactg cttagcgcaa tgtcgacact
    10741 ttttgccacc agggtcgcgg gctgaccatc acgaccaaac gtgacgttct ccagcacgat
    10801 atgagatggc gcggaaaaac ggtgatccat cgccccgaag gccagatgat agtcgctatt
    10861 ctcggaaacc catgcgctga tatgttctgc tccccagcgg gtttgcagaa gaaaatagag
    10921 gccagcgatc accactaaca gagcgatgag aatgtagaga agcagcttcc caataaattt
    10981 catggtcttc catcccgtga aatgcacata agggagttat gcacgattta cgcgcaatcc
    11041 tcaaggcggg aatggtgaaa gaggtgagac tgcggcggta attatcttac cgccgccagt
    11101 gaattactgt ttttctggtg ggaaaatcag attcagcacg atggcagtaa taccgcccgc
    11161 ggcgatcccg gaggagagca ggtttttcag ccattcaggg gcaaactgca aaatcagcgg
    11221 ctgctgagac acgcccagac caaccgccag cgacagcgcg ataatcagaa tcgcccgacg
    11281 gttcagcggc tcacgagaaa cgatacgcac accggaggcg gcgatggtgc caaacattac
    11341 aagcgttgcg ccgcccagaa ccggttctgg aatgtgttgt acaaaaccgc tcactgccgg
    11401 gaacagaccc agcacgatca acatcagcgc gacgacaaaa ccgacatagc ggctggcaac
    11461 accagtcaac tggatcactc cgttgttttg cccgaagcag gagttcggga aggtgttaaa
    11521 caccgccgaa acaaacgagt tcaggccgtt tgccagcacg ccgcctttca ggcgtttcat
    11581 gtacagcgga ccggacactg gctgttcgga aacgtcagag gtcgccgtga tatcgccaat
    11641 ggtttccagc gaagtgatca taaagaccag catcagcggc agcagcagac tccattcaat
    11701 gccaagaccg taatagagcg gcgttggcac cataatcagt tcttgcgtca tcggttcgtt
    11761 gctttctggc aacatgccca taaaccacgc cagcgcatat ccggccgcca tcgcaatcac
    11821 cagtgaggcc acgcgtaagt aagggttacg ttgacggtta agcaggataa ttaaggctaa
    11881 gaccacgcct gccagcagca gatttttcgg tgcgccgaag gtgttatcgc tcatggctgc
    11941 gtaaccgccg ccaatggacg ttaacccaac ctgaattagc gacaggccga taatcatcac
    12001 cacaacgcca gaaaccagcg gcgtaataat gcggcgcgcc agatgcagaa cgcgggagat
    12061 caccatctcg gtgcaacttg ccagcatcaa cgtgccgaac aaagccgcca tcatggtagg
    12121 aacatcagca ccaccggttt tcagcgcggt accgcccata atcagcgggg caacaaagtt
    12181 gaagctggtg ccctgaatag acaacagccc ggagccaacc ggaccccagg ccttaatttg
    12241 aataatcgat gccacaccgg aggcaaacag cgacatacta ataatgtgtt gcgtgtcttg
    12301 tgccggtaaa cccagcgcct ggcagattaa tagcgctggc gtgatcaccg caacgaacat
    12361 cgccagcaga tgctgacagg cggcaaacag ggtttgagga agcggcggac gatcttcaag
    12421 acggtaaatc agttcgctgt tttgagtctg cgcaaccggt tgcgcatttt ctgactcgag
    12481 ggtggaaaca gacatcgaaa acaatcccgt ggtagcaaag cgggcatttt agctgaccga
    12541 aagagaaaag caaacgtttg ccacatcctc atctaaatat ttaaatgaga taaatgcact
    12601 tttttatata acttttggta tttttctgct taaaatccat gccatttgtt cattaacagc
    12661 tgagaatttg acccaggaaa gcataactcg atacccgtca tagttcatga agttgccact
    12721 gcgcgatgaa gtatgacgag tatgaaagag tgatgcggat acaaaggagt aactatgttt
    12781 catctcgata ctttagcaac gcttgttgcc gcaacgctga cgttgttgct cgggcgtaag
    12841 ttggtccatt ccgtctcctt tttgaagaaa tacaccatac cggaacctgt tgcgggtggt
    12901 ttgttggtgg cgctggcgct actagtactg aaaaaaagca tgggctggga agtcaacttt
    12961 gatatgtccc tgcgcgatcc gttaatgctg gctttcttcg ccaccattgg cctgaacgcc
    13021 aacattgcca gtttgcgtgc cggtgggcgt gtggttggca tcttcttgat tgtggttgtt
    13081 ggtctgttgg tgatgcaaaa tgccattggc attggtatgg ctagcctgtt agggcttgat
    13141 ccgctgatgg ggctgttggc cggttctatt acgctttccg gcggtcacgg tacgggcgct
    13201 gcgtggagta aattgttcat tgaacgttat ggcttcacca atgcgacgga agtggcgatg
    13261 gcctgtgcaa cgttcggtct ggtgctgggc ggcttgattg gcggtccggt ggcgcgctat
    13321 ctggtgaaac actccaccac gccgaacggt attccggatg accaggaagt cccgacggcg
    13381 tttgaaaagc cggatgtggg ccgcatgatc acctcgctgg tgctgattga aactatcgcg
    13441 ctgattgcta tctgcctgac ggtggggaaa attgttgcgc aacttttggc tggcactgct
    13501 tttgaactgc cgaccttcgt ctgtgtactg tttgttggcg tgattctgag caacggtctg
    13561 tcaatgatgg gcttttaccg cgtctttgag cgtgcggtat ccgtgctggg taacgtaagc
    13621 ctgtcgttgt tcctggcgat ggcgctgatg gggctgaaac tgtgggagct ggcttcgctg
    13681 gcgctgccga tgctggcgat tctggtggta cagaccatct tcatggcgtt gtatgccatc
    13741 ttcgttacct ggcgcatgat gggcaaaaac tacgatgcgg cagtgctggc tgcgggtcac
    13801 tgtggttttg gcctcggtgc aacgccaacg gcaatcgcca acatgcaggc gatcactgaa
    13861 cgctttggcc cgtcgcacat ggcgtttttg gtggtgccga tggtcggtgc gttctttatc
    13921 gatatcgtca atgcgctggt gattaagctg tatttaatgt tgccgatttt tgccggttaa
    13981 aaatgaaaaa gcgtcggaag gaaccggaaa cgctacgtga acactgtcgc catatttttg
    14041 gtgatgagcc gccagtgctc tgtgtatggg aaacggaatt tgattacgca gatgccgaac
    14101 tcaaggcgct ggcggctaaa gagtggcagc agatcagcga atgggatttg agtgcttact
    14161 atgtactcaa tctcgtttat aacgaaccga tgcaaataga gttgtttcgc tatctgtttc
    14221 cgctctgcct ggcgcaatgg catgaaaccg ttttagctgg cggttatggt gatcacttcg
    14281 aagaatcctt aatgaaggca ttgtgtcggc cctatctctg gcaggagatg atgaatgctt
    14341 cccaacgcca gcaggtacgg caatttttac tggacaccgc gttgcagcgt atggataacg
    14401 agcgtggttt caataacgtt ctttgctggc tggcggtgtt caatactctg ggtggggccg
    14461 cgccgttaat tcgttctctc tggtcgcgct ggtgggcgct ggatacgcca ggaaaagcgg
    14521 tgtgtgctat tcagtatgcc gcgcatctaa tttatcccat agaagccaat ccgttatggt
    14581 cgcaagaatg gataggctgg gggcatccat taggccataa agatggctgg tcctctgata
    14641 atcgcgcttt tcttcgccaa atgttaacac ctgaaatgat tgtggctggc gtacaggcag
    14701 cggctgaaat tctgcgtggt gaaccggaag gcgcaatggc ggcgcgaatt gcacaggatg
    14761 cgtatgaggc aatggatatc ctcactatcc agattgaaga tttactgcgt gacttgtctt
    14821 gtgatgagtc tggtcatgcg ttggagtaac gttccgtctc cggcatccag cgttctatca
    14881 gggcttttgc ttgttgtggg taacgttcgt gaatatgtcg cgccaggcgc tgaacttccg
    14941 ggatcatcgc ctgatcgcgc agtaaatccg ccactttaaa ctcagcatta cccgtctgac
    15001 gcgtacctaa cagttcaccc ggtccgcgaa tctctaagtc tttctgtgca atcacaaaac
    15061 cgtcgttgct gtcgcgcagc acttgcagcc gaatttgcgc ggttttggag agcggcgttt
    15121 tgtagagcag cacgcagtga gaagccaccg caccacgacc tacacgcccg cgaagctggt
    15181 gtaactgcgc cagacccaga cgctcagggt tttcgataat catcaggctg gcgttaggca
    15241 catcaacacc gacttcaata acggtagtgg caactagcag atgtagctca ccttgtttaa
    15301 acgacgccat caccgcctgt ttctcggctg gtttcatccg cccgtgtacc aggccaacat
    15361 tcaattctgg tagcgccagt ttcaactctt cccaggtagc ttccgccgcc tgcgcttcca
    15421 gtaactctga ctcttcaatc aacgtacaaa cccagtacgc ctgacgacct tcagttatgc
    15481 aggcgtggcg cacgcggtca atgatgtcgg tacggcgggt atcaggaata gcgaccgtag
    15541 tcactggcgt gcggcctggc ggcagctcat ctatcaccga ggtatcgaga tcggcatacg
    15601 ccgtcattgc cagcgtgcgg gggatcggcg tggcggtcat gatcaactga tgcggatgga
    15661 agccttgctg ctggcctttc tcccataacg ccagacgctg atgcacgcca aagcgatgtt
    15721 gttcgtcgat aatcaccagc gccagaccgt taaactgcac ctgttcctgg aagatggcgt
    15781 gcgtaccaac aatcatctgc acctgaccgc tggcgatggc ttcctgctga gacagtcgcg
    15841 ctttgccttt ctgcttaccg gcgagccagc ccacttcgat accgagcggt tcaaaccagt
    15901 tgcggaagtt attggcgtgc tgttcggcga gtaattcggt tggtgccatc aatgccacct
    15961 gtttgccgtg ggcaatcgca cgcagcgcgg cgagggcggc gaccagcgtt ttaccggaac
    16021 ctacatcgcc ctgtaccagg cgcatcatcg gcacatccag cgccatatcg cgctcgatct
    16081 ccgccactac gcgtgcctgt gcgcccgttg gcttgaacgg taaggctgcg aggagtttat
    16141 ttttcagcgc atcattggcg ctcagcggct gggcatgaaa acgctgtgct ccggcacgta
    16201 aggctaacat gctgaggttg tgcgccagca gttcttccag aatcagacga cgttgcgctg
    16261 gatgctgccc ggtttccaga tcgctaagct gtagcgtcgg tggcgggcgg tgcaaagtgc
    16321 gcaacgcttc cggtagcgtc atcattcctt gtgacagttc cggcggcagg agttcttcaa
    16381 tggcgcaggt gtcgagcaga tccagcgcct ggtcggttaa tttacgcagc gtggcctgct
    16441 ttacgccttc cgttgttgga taaaccggcg tgagcgtttc ctgtaattct ggcgtgctga
    16501 gatcgccctg cacgcggtat tccgggtgga tcatctccgc accatattta ccgcgctttg
    16561 cttcgccata agccagtaca cggcggcccg tcgccaggct atttttcatt gccgcgctga
    16621 aattgaaaaa gcgcatggtg aggatgccgg aaccgtcgct gatctggcag gtcatcatcc
    16681 gccgaccgcc gaaggagata ttgcagttca gcacttcgcc ttccaccgtg gcataaacgc
    16741 ccggcagtag ttctccgatg gggtagagat gggtgcgatc ttcgtagcgc aggggaaggt
    16801 gtaagagtag atcctgcacg gtatgcaggt tgattttcgc cagtttgtta ctaagtgctg
    16861 cgccaacgcc cgttagggaa ctgagtggga cagcatctaa caggcgacct gtcatggcac
    16921 ttaccctgca gcctgcatag tagcccacca gtcggcatca gcttcgatct cgccttgctg
    16981 attgacgtgg ggataaggca ggcctttgcg ttttgcgact ttcgccagca ccggatagcc
    17041 gccttcaaac aacaggcgtt gttgctctgc ttccggcaac atgctgtttt cacgcaggta
    17101 catgcctgca ttttgccgct gacgctgggc ttcgtaaaga atgagggctg aggcaacgga
    17161 aacattcagc gactgcacca tgccgatcat cggaatgatg atgtcctgat ccgccagggc
    17221 caatgcttcc tgcgtgatgc ccgttttctc ctgtcccatc aaaatgcagg tcgggcgagt
    17281 gtaatcaatt tcgcggaaat cgacagcgtt atcagaaaga tgggttgcca gaatctgcat
    17341 gccctggcct ttgagatgag cgacggcatc gccaatggtg cggtgtgttt tcacctgtac
    17401 ccagctgtta ctacccgccg ctgccgaagc catggtgcgc atgcggctac caggccagac
    17461 ggcgtgaact tcatgtacgc caacggcatc tgcggtacga ataatcgcag aaacgttatg
    17521 aggtttgtgg acctgctcca tgcagacggt cagatcaggc tgccgcctgg cgagcatttc
    17581 gcagatgcgt gcataacgtg ttgggttcat aaaacattaa tttcggtttc gggtgacttt
    17641 aatcacgtct ggcatcacgc ggattttgcg catgatattc gccagatgca cacggtcacg
    17701 agcggtcaga cgaataaagg cgctgtagac gcgaccatct ttctcttccg tattcaaact
    17761 ttgaatattc gaggtcgtgg tgttaattgc cgccgtcagg tttgccagcg caccctgatg
    17821 attgaacatc tccaccttga tttcggtgat gaactcctgc gccgtctctt tatcccattc
    17881 cacagccata aacttctctg gctctttctg gtagccacgg atgttacggc aggattcatg
    17941 gtggatcacc agacctttac cggggctgac gtgggcgata atcgggtcgc caggaatggg
    18001 gcggcagcat ttcgcaaagg tgatcagcac gccatcagcg cctttgatcg gcagatgccc
    18061 gtggctttga gtcgccggtg gaatggaggc gtccccatgt tgtagatttt tcgcgaccac
    18121 cacgctcatt gcgttaccaa ggccgatttc tgccagcaga tcgtcaagcg ttgccagctt
    18181 catgcgatcc agctcgcgct gaatattttc ctgcgggatt tcattgagct tacggctacc
    18241 acccaaagca tggttgagca gacgacggcc caggcttaca gaatcatcac gcttgaggtt
    18301 tttcagcaac tgacgaattt tggcgcgcgc tttcgagcta acgacaaagt tcagccaagc
    18361 ggcattcggg cgagcgcccg gagcggtaat gatttcaacg gtctgaccgc tagtaagcgg
    18421 ctgcgacagc gggtaaggct ggcggtcgac gcgtgcgccc acgcaggcat ggccgatatc
    18481 ggtatgcact gcataagcga agtcgacggg cgttgcaccg gcaggcagct cgacaatgcg
    18541 cccttccggt gtgaaaacgt aaatctcatc cgggaagaga tcggatttaa cgctctcgat
    18601 aaattcaaac gaactaccgg cgctctgttg cagctccagc aggctttgca tccagcgctg
    18661 ggcgcggatt tgtgcggtag tactggtttc gccgtgctct ttataagccc agtgcgcggc
    18721 aacacccatc tccgccatct gatccatatc ttcggtacgg atctggacct caaccggcac
    18781 gccgtgcggg ccaatcatcg aggtgtgcaa cgactgatag ccgttcgctt ttggaatggc
    18841 gatatagtct ttcacgcggc ccggacgcgg cttgtacagg ctgtgcatct ggcccagcac
    18901 gcgataacag gtgtcagaat cattgacgat cacgcggaaa gcgtagatgt ccatgattga
    18961 gtgaaaacgc tgctctttga gcaccatttt acagtaaatc gaataaagat gcttttcgcg
    19021 accactgacg cggcacggta ttcccgcttc ctgcaaacgc ccttcgattt cagaaagaat
    19081 cttctggatc atctctttac ggttgccgcg cgcggctttc accacttctt tgattacgcg
    19141 ataacggttg ggatacagcg cctcaaaacc cagctcttcg agttcggttt taatgtggtg
    19201 gatacctaaa cggtgcgcca gcgggctgta aatttcgaga gtttcacggg cgatgcggcg
    19261 acgtttgtcc gggcgaagtg agcccagcgt gcgcatgttg tgggtacggt cggcaagttt
    19321 gatgaggatg acgcggatat cctgcaccat cgccataatc atcttgcgaa agttttcggc
    19381 ctgcgcctct ttcttatcgc ggaacttgag tttatcaagt ttcgacaccc cctctaccag
    19441 ctcggcgacg cttttaccaa aaagctgttc catatcctgg taggtggcgg gagtatcttc
    19501 aatcacgtca tgcagcagcg ccgccatcag cgtttcatag tcgagtttca tctcggccag
    19561 aatgcaggca accgctaccg ggtgcgtgat atagggttca ccgcttgaac gtgtttgccc
    19621 ctcgtgagca tcacgtgcaa cgagatacgc ctgccgcaga cgcttgattt ggtcttccgg
    19681 caggtaggtt tgaatcagtt gattcaggct ttcaaacaga tacaagggcg acccgctttg
    19741 tgattaacga cgaccttcag caatagcggt aacggcttgt aattcagcgg cttcctgctc
    19801 ttgctgttcc tggcgttcgc gaacgtcgag gatctggttg ttgatcagac cttcttcgat
    19861 ttcgcgcagc gcgattacag tggttttatc gttttcttcc ggtaccagcg gatcctttcc
    19921 gcctacctgc atctgacgag cgcgacgcgc ggcgaccagt accaggtcaa aacggttacc
    19981 aattttctct acagcgtcct gaacagttac gcgtgccata cttaaaaagc tccacaggtg
    20041 aagaaatgac tgggcatgat actgaaatca ggttcagtct gccaacaatt tgctgattaa
    20101 agcgtcatga cgctgctttt ggcggctcat gcgcagacgt tcggcgcgaa taatggtctt
    20161 caaatcggtc aacgcggtat cgaagtcatc attcacaatc aggtaatcat attcggcgta
    20221 atggctcatt tctgcaacag cttgcgccat acgctttgca atgacctctt cgctgtcctg
    20281 atcgcgaccg cgtagacggc ggtccagttc aattttagac ggcggtaaaa taaagatact
    20341 ccgcgcgtgc ggcatcttct ggcgaatttg ctgcgcgccc tgccagtcga tatcgagaaa
    20401 aacatcgaca ccggtcgcca gtacttgctc aatggcctca cgcgaagtgc catagtaatt
    20461 accaaaaact tctgcgtgtt cgaggaacgc atctctgcta atcatttctt taaattcatc
    20521 atgattaaca aagaaataat gttcaccgtg gacttcacca ggacgcggtt ggcgtgtggt
    20581 gtgtgaaaca gaaacctggg tgtcatacaa cggttgggtt tttaataaag cctgaatcag
    20641 gctggattta cccgcgccac tgggggcaga aacaatataa agcgtgcctt gagccatgag
    20701 tatctttcgt atgtgattag cgaaataaag cctacatacg agcttattat acacggcgcg
    20761 gccatgtgac gtagcccttg ctcacacttt ttgcgcaaag ttgttgcgtt ttgcgtagct
    20821 gtttccgcat ttgcctgtga tttactgcaa taaagacgat tctcaggaag cgtcctcgca
    20881 gactccgttt ttgccactcg gctttgcctt caccacgttg tatatctgcc agcctgacta
    20941 cagcggaggt gaagatgaaa gtatggatgg cgatattaat aagtatcttg tgctggcaat
    21001 catctgtgtg ggcggtctgt ccggcctggt cgccagccag agcacaggaa gaaattttcc
    21061 gcctgcaaca gcaaataaaa cagtgggacg atgactactg gaaggaagga aaaagtgagg
    21121 tggaagacgg tgtttacgat cagttaagcg cccgtcttac gcagtggcaa cgctgctttg
    21181 tgagcgagcc ccgcgacgtg atgatgccgc ctttaaatgg tgcggttatg catccggttg
    21241 cccatacggg tgtacgcaaa atggcggata aaaacgcatt aagtctgtgg atgcgagaac
    21301 gtagcgatct ttgggtgcag ccaaaagttg atggcgttgc ggtaaccctg gtttatcggg
    21361 acgggaaact gaacaaagca atcagtcgcg gtaatggcct gaaaggcgag gactggacgc
    21421 agaaagttag cttaatttcc gctgtgccgc aaaccgttag cgggccttta gccaacagta
    21481 cgcttcaggg ggaaatattt ctccagcgcg aggggcatat ccaacaacaa atggggggaa
    21541 taaatgcccg cgcaaaagtt gctggcctga tgatgcgcca ggacgatagc gacacgctga
    21601 attctctggg cgtttttgtc tgggcatggc cggatggacc gcagttaatg accgatcgtt
    21661 taaaggagct ggctaccgca ggttttactc taacgcagag gtatacccgt gcggttaaaa
    21721 atgctgatga ggttgcgcgc gtacgcaatg agtggtggaa agcgaaatta cccttcgtca
    21781 ccgatggcgt ggttgtgcga ggggcgaaag aacccgaatc ccgccattgg ctaccaggcc
    21841 aggcagaatg gctggtggcc tggaaatatc aacctgtagc tcaggttgcc aaagtgaagg
    21901 cgattcagtt tgcggtgggt aagagcggta aaatatcggt ggttgcgtca ctcgcacctg
    21961 tcatgctgga tgataaaaaa gtccagcggg tgaatattgg ttccgtcagg cgctggcagg
    22021 agtgggatat tgcgcctggt gatcagatcc tcgtcagcct tgccggtcag ggtattcctc
    22081 gcattgatga tgtggtctgg cgcggtgcag aacgtacaaa accgacacca ccagaaaacc
    22141 gctttaactc gttgacctgc tactttgctt ctgatgtttg tcaggaacag ttcatttcac
    22201 gcttagtctg gctggggtca aaacaggttc tggggctgga tggcattggt gaggccggct
    22261 ggcgcgcgct gcatcagact catcgctttg agcatatctt ttcctggctt ttattaacgc
    22321 cagagcaatt acagaacacg ccggggatcg cgaaaagtaa aagtgcgcag ctatggcatc
    22381 ggtttaatct ggctcgtaag cagcctttta ctcgctgggt gatggcaatg ggaataccgc
    22441 taacccgggc ggcgcttaat gccagtgatg aacggtcctg gtcgcaactt ttatttagca
    22501 cggagcagtt ctggcagcag ctgccgggga ctggatcggg gcgcgccaga caggttattg
    22561 aatggaagga aaatgcgcaa atcaagaagc tgggcagttg gctggctgcc cagcagatca
    22621 caggttttga accttagtgt ccttcgtggc tgtagtaaaa gaccggtaat cccagcttaa
    22681 ggcgtagcgc cagtaagcgg gcaaaaaagc cgaataccag ggtggagata attaccacat
    22741 catggttaga aacatagtgt tgcagcgcga tgtacagcac ggcggaggca aatgagacac
    22801 cggcatacaa ctctttctgg aataccagtg ggatacgttt acagaacata tcgcgcaaaa
    22861 cgccgccaaa cacgccggtg gttaccgccg cgacaacggc aataattggg ccgtgcccca
    22921 tatccagtgc aacctgtgcg ccgatgatag aaaagaccac cagtccaagc gcatcgagca
    22981 ccagaaacac tttgcgcagg taaggcatta caggagcaac gatagtggtg agtactgcgg
    23041 cggtagcgac gataatcaca tattccgggt gtttgaccca gccgagcgga tagtggccca
    23101 gcagaatatc gcgcactgac cctccgccaa ttgcggtggc ggtcgcaata ataattacgc
    23161 caaatgtatc catgcggcgt cgcccggccg ccagagcccc cgtcatggct tcggcagtaa
    23221 tgccaaccaa atacaaaatg tgtaacagca tggttctctc cctaaaaatc tgtctggcag
    23281 ggtagcgatt tgtgcgtatt gtcacgattg agatttttta aggtgagagg aaattgataa
    23341 cttgtagtta tgtgaaatcg ctattttctg tagcgtagat gcattcttct gacttcttgt
    23401 ctgagaaaat gcataagaaa aaataggtta aaattgaggg gtggaaaaga aaggtgcaat
    23461 gtttagccat tgtgttcacc gcacctggac tttggcttac gcatcgggta tctcaatcaa
    23521 caaaaagagt ttttagtttc tgctatttct tctctgtaat ttttacactg ttttccaact
    23581 gcttgatgct gacctgcggg gtcggcaact cctcaggcat ggttccgcca agttcctgaa
    23641 tggtttgcct caccttgcta cccacatcaa agtgtgtggt attagcttgc tgttttgaat
    23701 tcacgccatc ccgcttgagt ttttcttctg tttgggtagc tcgaaagaga ttagccgcca
    23761 gttctgttga acccatatga tccaggatct tctgactctt tttcagcccc ttcagttggt
    23821 ggatggcttt ctgatctaat ccaccataca gcccctggta accatgattc tgaaatatgg
    23881 cgaaatcagt agctgtcgct accgcagctt gctgtgctgc ctcaaccaat tgtttgttat
    23941 gttctttcaa ctcatttctc agaaacagac gcttttcgtc ttcacgaagt tgcttaaatg
    24001 cttcatcgtc agcaagctcc tgccgtcggg tctggatagc aaaataagtc tgccctgccg
    24061 caatgaccgg tttcgcaggg tcgccgtttt gtactaccag gtaacaggca tagcgggaga
    24121 gatgaacatc ttcaagttct cgttgagccc ctgaacctaa tacaaccatt ttggtggttt
    24181 caacgaaatg gtcagatgca gcttgattgc tggcttcgca cgcttgagtc gcgcgggcca
    24241 gtaccttttg aaaattgcgc cagtcccgat aatccagtaa cggtgccaat tctctagctg
    24301 accaaaattc ttgcccttca gtgccataat gccgtatttc ttcaaaaggt tgatgatgtt
    24361 cgttcattgc ccactccatg tgatatcgat tgcaaagaaa gtaaatctgt aactgtattt
    24421 atatacagtt gtgagtatct cacgttctca gtgtgctggg caaaaaaaca tcacaaaatc
    24481 tggagcaggc acgcaaaaca gaattgaagt tgcggggaag aaaaatgccg ctctgtttga
    24541 gtgggcggca tttgtgaagg ctgaaaagta agaattggcg ttacgagtta ttcaatgttc
    24601 tgaatctgct cgcgcatctg ctcaatcaac actttcagct caatcgcgga gtttgtcact
    24661 tcggcattga tagacttcga cgcaagagtg ttcgactcgc ggttgaattc ctgcatcata
    24721 aagtccagac gacgaccaac ggcttctttt ttcttcagaa tgttgtaggt ctctttgaca
    24781 tgcgcttcca ggcggtccag ttcttcggca acgtcaattc gttgtgccag cagaaccagc
    24841 tcctgctcca gacggttgtt ttccaactga acctgagcat cttccagctt cgcgaccaga
    24901 cgctcacgct gccattgcag gatttccggc atatgggcgc ggactttgac cacttcggcg
    24961 gtgacgcctt ccagacgctg ctcgatcaat gctttcagtg cctgaccttc ggtttcgcgc
    25021 gcgacaataa agtcgtccag cgtaccatcc agcgccgcga gaatttcagc ggcaatggcg
    25081 tcaagatcct gctcctgggc tgccatcacg cccggccagc gtagaatatc aaccgggttg
    25141 atttcccctt cgtcactctg catttttacc cagttcgcgg cagttaccag ctgtttagcc
    25201 agtttttcgt tgaggatcag ctcaccttgc gcgctaacat ctggctcata gcgcagggta
    25261 cattccactt taccgcgcgt caggcgagaa cgaatacgct cgcgaacgac aggttcaagg
    25321 ctacggaact gctccggcag acgaaagtaa gtttccagat aacgctggtt taccgagcgc
    25381 atttcccagg ttgcgctccc ccattcaccc ttgatttcac gccgggcgta ggcggtcata
    25441 ctgcggatca tagacgttcc tgtttataaa aggagaggtg gaaggattat agccatcgat
    25501 gccttgtcag gataggaata accgccggaa gtccgtataa tgcgcagcca catttgtttc
    25561 aagccggaga tttcaatatg cgtccagcag gccgtagcaa taatcaggtg cgtcccgtta
    25621 ccctgactcg taactataca aaacatgcag aaggctcggt gctggtcgaa tttggcgata
    25681 ccaaagtgtt gtgtaccgcc tctattgaag aaggcgtgcc gcgcttcctg aaaggtcagg
    25741 gccagggctg gatcaccgca gagtacggca tgctgccacg ttctacccac acccgtaacg
    25801 ctcgtgaagc ggcgaaaggt aagcagggtg gacgcacaat ggaaatccag cgtctgatcg
    25861 cccgtgctct tcgcgcggca gtagatttga aagcgctggg tgagttcacc attacgctgg
    25921 actgcgacgt gcttcaggct gatggtggca cgcgtaccgc gtcgattacg ggtgcctgcg
    25981 tggcgctggc agatgcgcta cagaagctgg tggaaaacgg caagctgaaa accaacccga
    26041 tgaaagggat ggtagccgca gtttctgtcg gaattgtgaa cggcgaagcg gtttgcgatc
    26101 tggaatacgt tgaagactct gccgcagaga ccgacatgaa cgtagtgatg accgaagacg
    26161 ggcgcatcat tgaagtgcag gggacggcag aaggcgagcc gttcacccat gaagagctac
    26221 tcaccttgtt ggctctggcc cgagggggaa tcgaatccat tgtagcgacg cagaaggcgg
    26281 cgctggcaaa ctgattttta aggcgactga tgagtcgcct tttttttgtc tgtagaaaag
    26341 taagatgagg agcgaaggca tgaaaccata tcagcgccag tttattgaat ttgcgcttgg
    26401 caagcaggtg ttaaagtttg gcgagtttac gttgaagtcc gggcgcaaaa gcccctattt
    26461 cttcaacgcc gggctgttta ataccgggcg cgatctggca ctgttaggcc gtttttacgc
    26521 tgaagcgttg gtggattccg gccttgagtt cgatctgctg tttggccctg cttacaaagg
    26581 gatcccgatt gccaccacta ccgccgtggc gctggcggag catcatgacc ttgacctgcc
    26641 gtactgcttt aaccgcaaag aggcaaaaga ccacggtgaa ggcggcaatc tggttggtag
    26701 cgcgttacaa ggacgcgtaa tgctggtaga tgatgtgatc accgccggaa cggcgattcg
    26761 cgaatcgatg gagattattc aggctaatgg cgcgacgctt gctggcgtgt tgatttcgct
    26821 tgatcgtcag gaacgggggc gcggcgagat ttcggcgatt caggaagttg agcgtgatta
    26881 caactgcaaa gtgatctcta tcatcaccct gaaagacctg attgcttacc tggaagagaa
    26941 gccggaaatg gcggaacatc tggcggcggt taaggcctat cgcgaagagt ttggcgttta
    27001 aagaaactcg ccggatgaaa agtcatccgg cgtcatatta ctgcaactgt gccgcaatta
    27061 gcggccagcg ggcgtcaaaa tcatccgtcg ggcggtattt aaattcgctg cggacaaaac
    27121 gtgacagcat accttcacag aaggctagga tctggcttgc cagcagggtt tcatcggtgg
    27181 tgtaaccttc accctcacgc attctctttt cacgcaatac ctgacgcagc tgcgcttcaa
    27241 tacgctcgaa cagctggttg atgcgccctt gcaggcgatc ctgttcaaac attagcgcat
    27301 gaccagtgag gatgcgggtc aggccaggat tacgctcacc aaaaccgaga agcagcaaca
    27361 caatcagacg caggcgcgct gtggtgtctt tctcatcttt cagaatcaga ttgatgcgag
    27421 taatcaggct atcttcgata aactcaatca ggctatcgaa catgcgggtc ttactgggga
    27481 agtggcgata cagtgccgct tcggaaacgc cgacagaggc ggccagtttt gccgtcgtga
    27541 tacgttggct tccatcgctg gattccagca tcagcgccag agactgaagt atttcctcgc
    27601 gacggttcct tttcgcagtt tgtttttctg ccatgttaca aaatacccct gaaaataagc
    27661 acttgccagg cggcacccac gctatgaccg caaacgaaat gtttacggct atgttatgac
    27721 gttattcgga tgcgtatgtg ttactgacga ccagagtgac caaagccgcc ttcaccgcgg
    27781 tcggtggcgt cgaaatcttc caccagatta aattcagcct gtactaccgg aacaaaaatc
    27841 atctgggcga tacgttcgcc aggttgaatg gtgaagctgt cctgaccacg gttccacacg
    27901 gaaatcatca actggccctg atagtcggaa tcgatcaatc ctaccaggtt accaagcacg
    27961 ataccgtgct tatgtcccaa tccggagcgc ggcagcatca ttgccgccag tgaaggatcg
    28021 gcaatatgaa tcgccagccc ggtcggaacc agcgtagtgt cacccggagc cagttctacg
    28081 gcgtcgtcga gacaggcacg caggtcaagt ccggcagagc cagaggtggc ataagtcggg
    28141 agcggaaatt ccttcccaac gcgcgggtcc agaatcttaa cgtcgatttt tttcatcata
    28201 acgggtcacg atctcgtcga gtaataattg gccaaggagc tctttgcgct caagcggtaa
    28261 gactttatct ccgtcctgcc agaaaaggtg taatgcgttg ttgtcgctgt taaatccttg
    28321 agttggctgg gaaacatcgt tcgcgcagat cagatcaagg tttttacgga tacgtttttg
    28381 ccgggcgtat tcttccacat tatttgtttc ggcggcaaat ccaacgacgt agggtcgatg
    28441 gtcttttagt gcggcaacgc ctgcgacgat atcggggttt ttaaccattt ttattgttaa
    28501 ttcatcaccc tgcgtggcct gctttttgat tttctctggg gccacggtag ctgcgcgata
    28561 atccgccacg gcggcgcagc cgataaaaat attttgctgc tgtacagaag cattcacggc
    28621 ggcttccatt tccagcgcgg tcatcacatc aacacgttta acaaacggtg gcgtcggtag
    28681 tgaaaccgga cctgatacca gcgtgacgtt cgcgccacga cgggcagcgg cggcggcgat
    28741 agcaaaaccc atcttgccgg agctgtgatt agagatataa cgcacgggat cgagcggttc
    28801 acgcgtcggg ccggcggtaa tcataatgtt cagatgtttc aggtcgttga cgggcgaaaa
    28861 atgcgctacc gccatatcca caatggttaa cggatcgagc attcgcccag gaccgatatc
    28921 accacaagcc tgactgccac tgtctggccc ccagatgagc aaaccacggg aagcaagcac
    28981 ctctaaatta tgctgcgtgg cagcggcacg gtacatctgc tggttcatgg cggggagcac
    29041 ggctacaggc gcaggtgtag ccagacaaat cgtcgatacc aggtcattcg ccattccggc
    29101 agcaacacgg gcaatcaaat ctgccgtggc aggggcgaga atcactaaat cagcccattt
    29161 acccagctca atatggccca tagcggcttc ggctgccggg tccagcaaac tgtcggaaac
    29221 gggataacca gaaaccgcct gcaagctaag tggggtgata aaggcttttg ccgcttcggt
    29281 catggctacg cggacgtcgg ccccgcgatc gcgcaaacga cgcaccagtt caggggtttt
    29341 ataggcagca ataccgccgc taacgccgag aacgattttt ttaccggcca ggctcatgat
    29401 gatttttcct gttgggtgac accagaagtt ggcgatttta tcacaatact tttgttgtcg
    29461 tgccttcacc tgagattcac tttgcgaggc gctttccagg attgaaaact ggccgtcgat
    29521 ttaacggaac ggctatgaca ggatgcgagc accacaaagg aggtgaaggt gaaaaacaat
    29581 gcacagctgt tgatgccgcg cgaaaaaatg ctgaagtttg gtattagcgc cttaactgat
    29641 gtcgagctgc tggcgctatt tctgcgtacc ggaacgcgcg gtaaagatgt attaaccctg
    29701 gcaaaagaga tgctggagaa tttcggctct ctttatggct tgttaacctc tgaatatgaa
    29761 caatttagtg gtgttcatgg aattggcgta gcgaaatttg cccagttaaa ggggattgct
    29821 gaactggcgc gacgttacta caacgtgcgg atgcgtgaag aaagcccttt actcagcccg
    29881 gagatgacgc gggaattttt acaaagccag ctcacgggtg aggagcggga gatctttatg
    29941 gtgatctttc tcgactccca acaccgggtt ataacgcata gccgtctttt ttccggcacg
    30001 ctaaaccatg ttgaagtcca tcctcgggaa attatccgcg aagcgataaa aataaacgcc
    30061 tcggcgctga tccttgcaca taatcaccct tcgggttgtg ctgaacccag taaagcggat
    30121 aaactcatta ctgaacggat aataaagagt tgtcagttca tggatttacg cgtgctcgac
    30181 catatcgtga ttgggcgtgg agagtatgtt tcttttgccg aacgcggctg gatttaaccc
    30241 gctatgcgcg atccttcggg atctttgtct gttcgggact tgagcacatc gctgagtcag
    30301 cgtatactac gccacctttg agaatctcgg gtttggcatt tgggcctggc aatcgagagt
    30361 tcacatagaa ctgcgatgac cgggctgtaa agcctgacga ggcgccaata ccccatacga
    30421 agctcgagct aatttgattt ttggagaata gacatgtccc gagtctgcca agttactggc
    30481 aagcgtccgg tgaccggtaa caaccgttcc cacgcactga acgcgactaa acgccgtttc
    30541 ctgccgaacc tgcactctca ccgtttctgg gttgagagcg agaagcgttt tgtcaccctg
    30601 cgcgtatctg ctaaaggtat gcgtgtaatc gataaaaaag gcatcgatac agttctggct
    30661 gaactgcgtg cccgtggcga aaagtactaa gtacttagag gaaataaatc atggctaaag
    30721 gtattcgtga gaaaatcaag ctggtttctt ctgctggtac tggtcacttc tataccacta
    30781 cgaagaacaa acgtactaag ccggaaaaac tggaactgaa aaaattcgat ccagttgttc
    30841 gccagcacgt gatctacaaa gaagcgaaaa tcaaataatt ctcgctttga tgtaacaaaa
    30901 aacctcgctc cggcggggtt ttttgttatc tgcttgcccc catattgact gcatctgttc
    30961 attcctggag atgctatgcc tgaattaccc gaagttgaaa ccagccgccg cggcatagaa
    31021 ccgcatctcg ttggtgcaac cattcttcat gcagtggtgc gcaacggacg cttgcgctgg
    31081 ccggtttcag aagagatcta ccgtttaagc gaccaaccag tgcttagcgt gcagcggcgg
    31141 gctaaatatc tgctgctgga gctgcctgag ggctggatta tcattcattt agggatgtct
    31201 ggcagcctgc gcatccttcc agaagaactt ccccctgaaa agcatgacca tgtggatttg
    31261 gtgatgagca acggcaaagt gctgcgctac accgatccgc gccgctttgg tgcctggctg
    31321 tggaccaaag agctggaagg gcataatgtg ctggcccatc ttggaccgga gccgcttagc
    31381 gacgatttca atggtgagta tctgcatcag aagtgcgcga agaaaaaaac ggcgattaaa
    31441 ccgtggctga tggataacaa gctggtggta ggggtaggga atatctatgc cagcgaatca
    31501 ctgtttgcgg cggggatcca tccggatcgg ctggcgtcat cactgtcgct ggcagagtgt
    31561 gaattgttag ctcgggtgat taaagcggtg ttgctgcgtt cgattgagca gggtggtaca
    31621 acgctgaaag attttctgca aagtgatggt aaaccgggct atttcgctca ggaattgcag
    31681 gtttacgggc gaaaaggtga gccgtgtcgg gtgtgcggta cgccgattgt ggcgactaaa
    31741 catgcgcagc gggcaacgtt ttattgtcgg cagtgccaga agtaattcat gcgcgccgga
    31801 tggcatacca tccggcataa acgctacgct aacttcgcca tcagcgcctg atggacattc
    31861 tccggcagga aatgggtgac atcgccctga tggcgcgcca cctctttcac caacgatgaa
    31921 gagataaacg accactcttt cgacggcatc agaaacacac tttccagttc cggcattaag
    31981 tggcgattca tatgcgccag ctgcatttca tattcaaaat ctgccaccgc acgcaggcca
    32041 cgaatcagca ccgtagcgtg ttgattacgg gcgaagttcg ccatcaaatc actaaacccg
    32101 accacttcca cgttccccag atgcgcggtt gcctgctgtg ccagtgccac acgctcttcc
    32161 agggtaaaca tcggtttttt actggggctg gcggcaatcg ccagaataac gtgatcgaac
    32221 atctgcgtgg cgcgcgtcac gatatcgata tgaccattgg taatgggatc gaaagtaccc
    32281 ggataaatcg cccgtttttg cataacaacc tcaatgcgtt ttcggtggca ggtaaggttc
    32341 cagcagttga agcagacgct gtagcgcgcc ctggttttga tacagtactt caacggcatg
    32401 acggccatag aaactacggt aatcggcgtc ggtgagtaaa gaggaaacct cttttgcaag
    32461 cgtagtggca tcggtaacgg taatcagccc gcttgcctgc tccagccgcg cgcaaatgtc
    32521 tttaaagtta aaagtatgcg gccccatcaa taccggaatt gcgtgtgcgg cagcttccag
    32581 cggattatgc ccaccacgtt caaccagtga accgccaaca aaggcgagat cggcaatgcc
    32641 gtacagtaac atcaactcgc ccatcgtatc gccaaccaca acctgcgtgc tggtggaggg
    32701 gacttcccct gaagagcgtg tgatatagct tagtccagcc tggcggacaa ggttaatcgc
    32761 atccgggaag cgttctggat gacggggtac caggatgagc aataaattcg ggaattgctg
    32821 taataaagcc tgatgtgcgg caatgaccac gctctcttca ccttcgtgag tgctggtggc
    32881 aatccatacc gggcggtgtg gtgcccactg gcggcgcagc gtcacggctt tagcagccaa
    32941 ctgcggcgtt acagaaatat cgaatttcag gctaccggta acggttacct ggttattttt
    33001 cgcgcccagc gccacaaaac gtgcaccatc ttcttcattt tgggcggcaa tcagcgtaat
    33061 acgacgcagc aagcgacgga cgaatttacc cagtttggca taacctgcgg ccgagcgggc
    33121 agagagtcgc gcgttagcga tcaccagcgg aattttacgt ttatgtagcg ccgcaatcag
    33181 gttaggccat agttcggttt ccataatcaa caccagttta gggtcgactt tattcaggaa
    33241 acggttgagt gcatcgggca gatcatacgg cagataaacg tgctgaacat ccttcccgaa
    33301 agccgattgt acgcgctccg aaccggttgg cgtcatggtt gttacggtaa taggtaaatc
    33361 aggataacga tgacgcagcg cgcgcaccaa cgggatcgcc gccagagttt caccgacgga
    33421 gacggagtgc agcataatgc cgcctggttt tagcggatgg cggtaaaaac cgtaacgttc
    33481 accccagcgt tttcgatagg ccggagcctt acgtccgcgc acccagagcc gtatccagat
    33541 cagcggctga ataaggtaga gaagggcggt gtaaagcaat tcgagcatag taaatagctg
    33601 acttatggat gtgctgggga ttctatgtat ttagctgtgg ctttaccatt acttttcccg
    33661 tttttgactt aaatagcaat ttacccgcca attcttgctg aacatctata ctttttttgt
    33721 atttttatgc gattcattga tttctgctgt acgtgcagga aggaatcgca gagcaacagt
    33781 gaaagaatat ggttgctaat gattttattt gattgatttc tttattgaaa atcaacaatg
    33841 tgcttaaaaa tcattcgggt cggattgccg cgaaagaagt gatacactag cgcgtcaata
    33901 tcagtgcggt atcagtattc aggtagctgt tgagcctggg gcggtagcgt gctttttctg
    33961 cttcacctta atcggataat ctcaacaaaa agagttactt gtggataagc catttcgaag
    34021 aattttgctc attaagatgc gttttcatgg ggatatgtta ttaactactc ccgtcattag
    34081 ttcgctgaaa aaaaattacc ctgacgcaaa aatcgatgtg ctgctttatc aggacaccat
    34141 cccgatcctg tctgaaaatc cagagattaa cgcgctctac ggcataaaaa ataaaaaagc
    34201 aaaagcctca gaaaaaattg ccaacttttt tcatctcatc aaggtattac gtgccaataa
    34261 gtatgacctt atcgtcaatc ttaccgatca atggatggtt gctatactgg ttcgcttatt
    34321 aaatgcccgt gtgaaaattt cccaggatta tcatcatcgg cagtctgctt tttggcgtaa
    34381 aagtttcacc catttggtgc cgttgcaggg tggaaatgtg gtggaaagta acttatccgt
    34441 gctgacacca ttgggacttg attcgttggt gaagcagaca accatgagtt acccgcctgc
    34501 aagctggaaa cgtatgcgtc gcgaacttga tcacgctggt gttggacaaa attatgtggt
    34561 tatccaacct acggcgcggc aaatcttcaa atgctgggac aacgccaagt tttccgctgt
    34621 gattgatgcc ttacatgctc gtggttatga agtcgttctg acgtccggcc cagataaaga
    34681 cgatctggcc tgcgtcaatg aaattgcgca aggatgccag acgccaccag taacggcgct
    34741 ggctggaaag gtgaccttcc cggaacttgg tgcgttaatc gatcatgcgc agctgtttat
    34801 tggcgttgat tccgcaccgg cgcatattgc cgctgcagtt aatacgccgc tgatatcgct
    34861 gtttggtgcg acagaccata ttttctggcg tccttggtca aataacatga ttcaattctg
    34921 ggcgggagat taccgggaaa tgccaacgcg cgatcagcgt gaccgaaatg agatgtatct
    34981 ttcggttatt ccggcggcag atgtcattgc tgctgtcgat aaattactgc cctcctccac
    35041 gacaggtacg tcgttatgat cgttgctttt tgtttatata aatattttcc ctttggcggt
    35101 ttgcagcgcg attttatgcg tattgctcag acagtcgccg cccgaggtca tcatgttcgg
    35161 gtttataccc agtcgtggga aggcgaatgc cctgatgtat ttgaactgat caaagtgccg
    35221 gttaaatcgc ataccaatca cgggcgcaat gcggagtatt ttgcctgggt gcaaaaacat
    35281 ttacgcgaac atcccgtcga taaagtcgtt ggattcaaca aaatgccggg gctggacgtt
    35341 tattatgccg ctgatgtttg ttatgccgag aaagtcgcgc aggaaaaagg ctttttctat
    35401 cacctgacgt cacgttatcg ccattatgcc gcctttgagc gggcaacctt cgaacagggc
    35461 aagccgacac agctgctgat gctgacagat aagcaaatcg ccgatttcca gaaacattat
    35521 cagactgaag cggagcgttt tcatattcta ccaccgggga tttatcctga tcgtaaatat
    35581 agccagcagc cagctaatag ccgtgaaatc ttccgtaaga agaatggaat aaccgaacaa
    35641 caatatttat tgttgcaggt cggttcagac ttcacgcgta aaggtgtcga tcgttccatt
    35701 gaagcacttg cttcgttacc ggattcgctg cgccacaaca cattgctata tgttgttgga
    35761 caggataaac cgcgaaaatt tgaggcgctg gcagaaaaac gcggcgtgcg cagtaatgtt
    35821 cacttcttct cggggcgcaa cgatgtctcg gaattaatgg cggcggcgga tttattactg
    35881 catcctgcct accaggaagc agcgggaatt gtgctactgg aagcgattac tgcaggatta
    35941 ccggtactaa caacagccgt ttgtggctat gcgcattata ttgtcgacgc caattgcggc
    36001 gaggctattg ctgagccatt ccgccaggaa acattgaatg agattttacg caaagcgtta
    36061 acgcaatctt cattgcgcca ggcctgggcg gaaaatgcgc gacattatgc tgatacacaa
    36121 gatttataca gtctgccaga gaaagcggcg gatatcataa cgggtggtct ggatggttga
    36181 acttaaagag ccgtttgcca cgttatggcg cggtaaagat ccttttgagg aagttaaaac
    36241 cttgcagggt gaggtttttc gtgaactgga aactcgccgc actctgcgat ttgaaatggc
    36301 gggcaaaagc tattttctca aatggcatcg cggcacgacc ctgaaagaga taatcaaaaa
    36361 tttactctca ttgcggatgc cagtattagg cgcagaccgc gaatggaatg cgattcatcg
    36421 actgcgggat gtcggcgttg atactatgta tggggtggca tttggcgaaa aaggcatgaa
    36481 tccgctgacc agaacttcat ttattattac cgaagatctg acaccaacca taagtcttga
    36541 agattactgt gctgactggg cgactaaccc tccagatgtt cgcgtaaagc gtatgcttat
    36601 taagcgtgtc gcgacgatgg tgcacgatat gcatgctgcg ggcattaacc accgtgactg
    36661 ttatatctgt catttcctgc tgcacttgcc tttttccggt aaggaagagg agttaaaaat
    36721 ttcggtaatt gacctgcacc gggcgcagct tcgcacgcgc gttccacgtc gttggcggga
    36781 taaagatctt attgggcttt atttttcttc gatgaatatc ggcctgactc agcgggatat
    36841 ctggcggttt atgaaagtgt attttgccgc cccgcttaaa gacattctca agcaggaaca
    36901 aggactgctg tcgcaagcag aagcaaaagc cacaaaaatc agggaaagaa cgattcgaaa
    36961 atcgttgtaa cccgtaactt atttttgcca aaattttgga tacagaataa atatgtctca
    37021 actcaatgat agtgacatca tcctttttga gtataatttt cattatcaaa atataagatc
    37081 taaaaatact cttgatatag catttggtat tgacagaaat tttctttttg gatgtggtgt
    37141 agcc
//
LOCUS       DAZKIP010000035        36247 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZKIP010000035 DAZKIP010000000
VERSION     DAZKIP010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.035"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            84..1766
                     /gene="ydeN"
                     /locus_tag="ACHOUK_002000"
     CDS             84..1766
                     /gene="ydeN"
                     /locus_tag="ACHOUK_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFP9897460.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQVPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPEQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1818..2975
                     /gene="ydeM"
                     /locus_tag="ACHOUK_002001"
     CDS             1818..2975
                     /gene="ydeM"
                     /locus_tag="ACHOUK_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFP9897461.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKLGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNDETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2941..3063)
                     /locus_tag="ACHOUK_002002"
     CDS             complement(2941..3063)
                     /locus_tag="ACHOUK_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9897462.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3266..4951
                     /gene="yddA"
                     /locus_tag="ACHOUK_002003"
     CDS             3266..4951
                     /gene="yddA"
                     /locus_tag="ACHOUK_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFP9897463.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGMLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
     gene            4989..7361
                     /gene="yddB"
                     /locus_tag="ACHOUK_002004"
     CDS             4989..7361
                     /gene="yddB"
                     /locus_tag="ACHOUK_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFP9897464.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKFKRFNADDSSVKLGYRTTRSDWLTSHINENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGMAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHISQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7418..10201
                     /gene="pqqL"
                     /locus_tag="ACHOUK_002005"
     CDS             7418..10201
                     /gene="pqqL"
                     /locus_tag="ACHOUK_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFP9897465.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLLQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMEELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNEVAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLTKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            10563..11963
                     /gene="gadB"
                     /locus_tag="ACHOUK_002006"
     CDS             10563..11963
                     /gene="gadB"
                     /locus_tag="ACHOUK_002006"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFP9897466.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
     gene            12119..13654
                     /gene="gadC"
                     /locus_tag="ACHOUK_002007"
     CDS             12119..13654
                     /gene="gadC"
                     /locus_tag="ACHOUK_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFP9897467.1"
                     /translation="MATLVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            13785..15104
                     /gene="digH"
                     /locus_tag="ACHOUK_002008"
     CDS             13785..15104
                     /gene="digH"
                     /locus_tag="ACHOUK_002008"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFP9897468.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            15481..16863
                     /gene="dosC"
                     /locus_tag="ACHOUK_002009"
     CDS             15481..16863
                     /gene="dosC"
                     /locus_tag="ACHOUK_002009"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFP9897469.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPPIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            16888..19277
                     /gene="dosP"
                     /locus_tag="ACHOUK_002010"
                     /gene_synonym="pdeO"
                     /pseudo
     CDS             16888..19277
                     /gene="dosP"
                     /locus_tag="ACHOUK_002010"
                     /gene_synonym="pdeO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
     gene            19535..20116
                     /gene="ddpX"
                     /locus_tag="ACHOUK_002011"
     CDS             19535..20116
                     /gene="ddpX"
                     /locus_tag="ACHOUK_002011"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFP9897470.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHVS"
     gene            20130..21680
                     /gene="ddpA"
                     /locus_tag="ACHOUK_002012"
     CDS             20130..21680
                     /gene="ddpA"
                     /locus_tag="ACHOUK_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9897471.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASNDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            21682..22704
                     /gene="ddpB"
                     /locus_tag="ACHOUK_002013"
     CDS             21682..22704
                     /gene="ddpB"
                     /locus_tag="ACHOUK_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9897472.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPRGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            22701..23597
                     /gene="ddpC"
                     /locus_tag="ACHOUK_002014"
     CDS             22701..23597
                     /gene="ddpC"
                     /locus_tag="ACHOUK_002014"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFP9897473.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            23594..24580
                     /gene="ddpD"
                     /locus_tag="ACHOUK_002015"
     CDS             23594..24580
                     /gene="ddpD"
                     /locus_tag="ACHOUK_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9897474.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDTVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARDSGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            24573..25499
                     /gene="ddpF"
                     /locus_tag="ACHOUK_002016"
     CDS             24573..25499
                     /gene="ddpF"
                     /locus_tag="ACHOUK_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFP9897475.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMMFQDPLSSLNPRLPVWRI
                     ITEPLWIAKHSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGEAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     RERCPLATSGCEVRQSLTTRADGREIRCWRAL"
     gene            complement(25555..25986)
                     /gene="osmC"
                     /locus_tag="ACHOUK_002017"
     CDS             complement(25555..25986)
                     /gene="osmC"
                     /locus_tag="ACHOUK_002017"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFP9897476.1"
                     /translation="MTIHKKGQAHWESDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            26331..26546
                     /gene="bdm"
                     /locus_tag="ACHOUK_002018"
     CDS             26331..26546
                     /gene="bdm"
                     /locus_tag="ACHOUK_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFP9897477.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            26648..26785
                     /gene="sra"
                     /locus_tag="ACHOUK_002019"
     CDS             26648..26785
                     /gene="sra"
                     /locus_tag="ACHOUK_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFP9897478.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            26942..28639
                     /gene="maeA"
                     /locus_tag="ACHOUK_002020"
     CDS             26942..28639
                     /gene="maeA"
                     /locus_tag="ACHOUK_002020"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFP9897479.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDRDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            28773..29783
                     /gene="adhP"
                     /locus_tag="ACHOUK_002021"
     CDS             28773..29783
                     /gene="adhP"
                     /locus_tag="ACHOUK_002021"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFP9897480.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            29929..30213
                     /gene="yddM"
                     /locus_tag="ACHOUK_002022"
     CDS             29929..30213
                     /gene="yddM"
                     /locus_tag="ACHOUK_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFP9897481.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAAKTVDVSRLRRLVAQ"
     gene            complement(30441..31094)
                     /gene="fdnI"
                     /locus_tag="ACHOUK_002023"
     CDS             complement(30441..31094)
                     /gene="fdnI"
                     /locus_tag="ACHOUK_002023"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFP9897482.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(31087..31971)
                     /gene="fdnH"
                     /locus_tag="ACHOUK_002024"
     CDS             complement(31087..31971)
                     /gene="fdnH"
                     /locus_tag="ACHOUK_002024"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFP9897483.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQSGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(31984..35031)
                     /gene="fdnG"
                     /locus_tag="ACHOUK_002025"
     CDS             complement(31984..35031)
                     /gene="fdnG"
                     /locus_tag="ACHOUK_002025"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(34444..34446),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFP9897484.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKEHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            35263..36144
                     /gene="yddG"
                     /locus_tag="ACHOUK_002026"
     CDS             35263..36144
                     /gene="yddG"
                     /locus_tag="ACHOUK_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFP9897485.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         9381 a         8885 c         8821 g         9160 t
ORIGIN      
        1 tatgtgacta acttcactta catccactta tttctcttcg taaaattact ttggaattaa
       61 gtacaataag aagaggaaca tttatgaagt ctgcattaaa gaaaagtgtc gtaagtacct
      121 cgatatcttt gatactggca tctggtatgg ctgcatttgc tgctcatgcg gcagatgatg
      181 taaagctgaa agcaaccaaa acaaacgttg ctttctcaga ctttacgccg actgaataca
      241 gtaccaaagg aaagccaaat attatcgtac tgaccatgga tgatcttggt tatggacaag
      301 ttccttttga taagggatct tttgacccaa aaacaatgga aaatcgtgaa gttgtcgata
      361 cctacaaaat agggatagat aaagccattg aagctgcgca aaaatcaacg ccgacgctcc
      421 tttcattaat ggatgaaggc gtacgtttta ctaacggcta tgtggcgcac ggtgtttccg
      481 gcccctcccg cgccgcaata atgaccggtc gagcgcccgc ccgctttggt gtctattcca
      541 atactgatgc tcaggatggt attccgctaa cagaaacttt cttgcctgaa ttattccaga
      601 atcatggtta ttacactgca gcagtaggta aatggcactt gtcaaaaatc agtaatgtgc
      661 cggtaccgga agataaacaa acacgtgact atcatgacaa cttcaccaca ttttctgcgg
      721 aagaatggca accgcaaaac cgtggctttg attactttat gggattccac gctgcaggaa
      781 cggcatatta caactcccct tcactgttca agaatcgtga acgtgtcccc gcaaaaggtt
      841 atatcagcga tcagttaacc gatgaggcaa ttggcgttgt tgatcgtgcc aaaacacttg
      901 accagccttt tatgctttac ctggcttata atgctccgca cctgccaaat gataatcctg
      961 caccggagca atatcagaag caatttaata ccggtagtca aacagcagat aactactacg
     1021 cttccgttta ttctgttgat cagggtgtaa aacgcattct cgaacaactg aagaaaaacg
     1081 gacagtatga caatacaatt attctcttta cctccgataa tggtgcggtt atcgatggtc
     1141 ctctgccgct gaacggggcg caaaaaggct ataagagtca gacctatcct ggcggtactc
     1201 acaccccaat gtttatgtgg tggaaaggaa aacttcaacc cggtaattat gacaagctga
     1261 tttccgcaat ggatttctac ccgacagctc ttgatgcagc cgatatcagc attccaaaag
     1321 accttaagct ggatggcgtt tccttgctgc cctggttgca agataagaaa caaggcgagc
     1381 cacataaaaa tctgacctgg ataacctctt attctcactg gtttgatgag gaaaatattc
     1441 cattctggga taactaccac aaatttgtcc gtcatcagtc agacgattac ccgcataacc
     1501 ccaacactga ggacttaagc caattctctt atacggtgag aaataacgat tattcgcttg
     1561 tctatacagt agaaaacaat cagttaggtc tctacaaact gacggatcta cagcaaaaag
     1621 ataaccttgc cgccgccaat ccgcaggtcg ttaaagagat gcaaggcgtg gtaagagagt
     1681 ttatcgacag cagccagcca ccgcttagcg aggtaaatca ggagaagttt aacaatatca
     1741 agaaagcact aagcgaagcg aaataactaa accttcatgc ggcggatttt tccgccgcct
     1801 tattgagcga gatagcgatg cacgttacag ccaagccctc cagttttcaa tgtaatctca
     1861 aatgtgatta ctgtttttac cttgaaaaag agtcgcagtt tactcatgaa aaatggatgg
     1921 atgacagcac tctgaaagag ttcatcaaac aatatatcgc agcgtctggc aatcaggtct
     1981 attttacctg gcaaggcggt gaacccactc tggctggcct ggattttttc cgtaaagtta
     2041 ttcactatca acaacgctat gcaggccaaa aacgtatttt taatgcatta caaacgaatg
     2101 gcattttatt gaataatgaa tggtgtgcct ttctcaaaga acatgagttt ctggttggta
     2161 tctcgatcga tggaccccag gagctacatg accgttacag acgcagtaat tcaggtaacg
     2221 gtacttttgc aaaagtgata gcagccatcg agcgtctgaa atcatatcaa gtagagttta
     2281 atacgttaac cgtcattaat aacgttaatg tccattaccc tcttgaggtt tatcattttt
     2341 taaaatctat cggcagtaaa catatgcaat ttatcgaatt gctcgaaacc gggacgccga
     2401 atattgattt cagtggtcat agtgagaaca cattccgtat cattgatttt tctgtgcctc
     2461 ccacggctta tggcaagttt atgtcaacca tttttatgca atgggttaaa aacgatgtgg
     2521 gtgaaatttt catccgtcag tttgaaagct ttgtcagccg ttttttgggg aatgggcata
     2581 ccagttgtat tttccaggag tcctgcaagg ataatctggt tgttgaaagt aatggagata
     2641 tttacgaatg cgatcatttt gtctatccac agtacaaact tggaaacatt aataaatctg
     2701 aacttaaaac gatgaacagt gtacaactga cagcgcaaaa aaaacggatt tcagcgaaat
     2761 gtcagcaatg tgcatataaa cctatctgca atggcggttg ccctaagcat cgtattacta
     2821 aagtaaacga tgagactgtt tcctattttt gcgaaggtta taaaatcctt ttttcaacca
     2881 tggtacctta tatgaacgcc atggtggagt tagccaagaa cagagtaccg ctttaccaca
     2941 ttatggatgt tgcaaaacaa atggagaata attaataacg ctctctttta gctccattct
     3001 tagcctcctt cttatgacct taacaaggca cccgacataa attcctgtcg ggtgtttcgt
     3061 catcatcatc ttcccttgcg aaaatcttgg cttacgtggt atgcgtcagg gcattagcaa
     3121 tacccttgat tatcaatagc ataaccataa ccactgaaaa ttaaaaattt ctatatattt
     3181 catattgtta aatgatactt aaataaattt catcagtaaa tcattgccat agataatcat
     3241 tggatggcac aatcaatttt cgctaatgat aaccattccc attacgtttt gtatgttgat
     3301 agcaaagtat ctctgtctgc tgaagccctt ttggctgcgt aagaataata aaacttcagt
     3361 gttgttaatc attattatcc tcgccatgat ccttggcgtg gttaaaattc aggtctggtt
     3421 aaacgactgg aataatgatt ttttcaatgc gctaagccag aaagaaaccg ataagctttg
     3481 gcagcttgtc ctttggttcc ctgccctgct ggggatcttt gtattaatat ccgtaaacaa
     3541 aacatggtta attaaactac tgactattcg ctggcgcgaa tggttaacag attactacct
     3601 taatcgctgg tttgcagata aaaactacta cttcacgcag atctacggcg agcataaaaa
     3661 tacagataac cctgaccaac gtatcgctga agacattctc ttactgatca gcaaaacgct
     3721 cagtctttca tttggcttca tccagtcact tagcatgctg atcaccttta ccgttattct
     3781 ttggcaaagc gcgggtatgc tctcattcac tgttggtgga acagaatgga atatccaggg
     3841 gtatatggtc tataccgttg tgctcattgt gatcggtgga actttattta cccataaggt
     3901 gggtaaacgt attcgtccgc ttaatgtgga aaaacaacgt agcgaagcga catttcgaac
     3961 taatcttgtg cagcataata agcaggcaga actgattgcg ctaagcaatg ctgagtctct
     4021 acaacgccag gagttgagcg acaattttca taccattaaa gagaactggc atcgtttaat
     4081 gaatcgtcag cggtggcttg attactggca gaatatttat tcgcgctcgc ttagcgttct
     4141 tccctacttt ctgttattgc cgcagtttat tagcgggcag atcaatctgg gcggactgat
     4201 gaaatcgcgc caggcattta tgctggtatc gaacaatcta agctggttta tttataaata
     4261 tgatgaactt gctgaactgg ctgcggttat cgatcgcttg tatgagttcc atcaactcac
     4321 cgaacagcgc cctacgaata agcctaaaaa ttgccaacat gcggtacaag tggctgacgc
     4381 gagtattcgt actcctgata ataagatcat attagagaac ctgaactttc atgtttcgcc
     4441 aggcaaatgg ctattactga aaggttactc tggtgcggga aaaaccacac tgcttaaaac
     4501 attatcccac tgctggccgt ggtttaaagg tgatatttct tctcctgccg acagttggta
     4561 tgtgtcacaa acaccgttaa tcaaaaccgg cttactgaaa gagattattt gtaaagcact
     4621 tcccctgccc gtagacgata aatcattgag cgaagtactg catcaggttg gtcttgggaa
     4681 attggctgcg cgtattcatg accacgatcg ctggggagat attctttcca gcggcgaaaa
     4741 acaacgtatc gccctggcac gattaatttt acgacgtccg aaatggatat ttcttgacga
     4801 aactacctct catcttgagg aacaagaggc tatccgctta ctgcgtctag tgcgtgaaaa
     4861 actacccaca agcggcgtca ttatggttac acatcaaccc ggtgtctgga acctggccga
     4921 tgatatttgt gacattagcg cggttttata aaaacaataa aagccctgct gtacggagat
     4981 ataaataaat gaagcgagtt cttattcctg gcgtcatttt atgtggcgct gatgtggcgc
     5041 aggccgtcga tgacaaaaac atgtacatgc atttttttga agagatgacg gtctatgctc
     5101 ctgtccctgt acccgtaaac ggcaacacgc attacaccag tgaaagcatc gagcgtttac
     5161 cgaccgggaa tggcaatatc agcgatctgc tgagaaccaa ccctgcggta cgcatggatt
     5221 caacgcaaag tacctcgttg aaccagggag atattcgccc ggagaaaatc tctattcacg
     5281 gtgcgtcgcc ctatcagaat gcctatttga ttgacggtat tagtgccact aataacctga
     5341 acccagcgaa tgagtccgat gccagtagtg caaccaatat tagcgggatg tcacaggggt
     5401 attatcttga tgtcagctta ctggacaatg tgacgcttta tgacagtttt gtgccggttg
     5461 aattcggtcg cttcaatggc ggggtaattg atgcaaagtt caaacgcttc aacgctgatg
     5521 atagcagcgt gaaactgggt tatcgcacta cgcgttcgga ctggttaaca tcgcatatca
     5581 atgagaataa caagagcgca tttaatcaag gctcttcagg aagtacttat tactccccag
     5641 attttaaaaa gaacttttat accttgtcgt ttaatcagga actcgctgat aactttggcg
     5701 ttaccgccgg tttatcgcgc cgccagtctg atatcacccg cgcggattat gtttcgaatg
     5761 acggcattgt cgccggtcgg gcacagtata aaaacgttat cgatactgca ttgagcaaat
     5821 ttacctggtt tgccagcgac cgctttaccc acgatttaac cttaaaatat accggctcca
     5881 gccgtgatta taataccagc accttcccgc agtctgatcg cgaaatgggt aataaatcct
     5941 atggtatggc atgggatatg gatacgcagc tcgcatgggc caaactacgt accaccgttg
     6001 gttgggatca tattagtgat tatacccgtc acgatcatga catctggtac accgaacttt
     6061 catgtacata tggtgatatt acagggcgtt gcacccgtgg cggattagga cacatttccc
     6121 aggctgtaga taattacacc ttcaaaacac gcctggactg gcaaaaattc gccgtgggta
     6181 atgtttcgca tcaaccctac ttcggcgcgg aatacatcta ttccgatgcg tggactgaac
     6241 gccataacca gtctgaatcc tatgtgatta atgctgccgg aaagaaaact aaccatacca
     6301 tttaccataa aggtaaaggc agcctgggaa ttgacaacta cacactgtat atggcggatc
     6361 gcattagctg gcgtaatgtg tcattaatgc ccggcgtgcg gtatgactat gacaactatc
     6421 tgtcaaacca caatatctcc ccgcgcttta tgacggaatg ggatattttt gctgatcaaa
     6481 cctcaatgat tacagcaggt tataaccgtt actatggcgg gaatattctt gatatgggat
     6541 tacgtgatat ccgcaatagc tggacggaat cggtatcagg taataaaacc ctgacgcgtt
     6601 atcaggattt gaaaacgcct tataacgatg aactggcaat gggattgcag caaaaaatcg
     6661 gtaagaacgt tattgcgcgc gcaaactatg tttaccgtga agcgcatgat caaatcagca
     6721 aaagcagtcg taccgacagc gcgactaaaa ccaccattac tgaatataac aacgacggca
     6781 aaaccaaaac gcattcgttc aacctcagtt ttgaactggc cgaacccctg catatcagcc
     6841 aggtagatat taacccgcaa attgtcttta gctatatcaa gagcaagggc aacttgtcgt
     6901 taaacaatgg ttatgaggag agcaataccg gtgataacca ggtggtttat aacggtaatc
     6961 ttgtctctta cgatagcgtt ccagtggcag attttaataa cccattaaag atctccttaa
     7021 acatggattt cacgcatcaa ccgagcgggt tggtgtgggc gaatacgctg gcctggcaag
     7081 aagcgcgtaa agctcgcatt atcctgggta agacaaatgc gcaatacatc agcgaatatt
     7141 cagattacaa gcagtatgtt gacgaaaaac tggatagcag cctgacctgg gacacccgct
     7201 tgtcctggac gccacaattt ctgaaacaac aaaacctgac gatcagtgcc gatattctca
     7261 atgtactgga tagcaaaacc gcggttgata caacgaatac cggtgtggcg acctacgcca
     7321 gtggccgtac tttctggctt gatgtcagca tgaaatttta atagttcgga ttttacttaa
     7381 ccgggtaaat tcgcccggtt ttcgcatgga gataattatg agaaacctct gtttcttact
     7441 gacgttagtg gcaactctgt tgctccccgg gcggctgatt gccgccgcct taccgcagga
     7501 tgaaaagtta attaccgggc aactggacaa tggcttgcga tatatgattt atccgcatgc
     7561 tcatccaaag gatcaggtaa atttatggct gcaaattcat accggttcat tgcaggaaga
     7621 agacaatgag cgcggcgtgg ctcattttgt agaacatatg atgtttaacg gcacaaaaac
     7681 atggccgggt aataaagtca tcgaaacatt tgagtcaatg ggcctgcgtt ttggtcgcga
     7741 tgttaatgcc tataccagct atgacgaaac ggtgtatcag gtgagtttgc caactacgca
     7801 gaaacaaaat ctgcaacaag tgatggcaat cttcagtgaa tggagtaatg ccgcaacctt
     7861 tgaaaaactc gaagtagacg ctgaacgcgg cgtaattact gaggaatggc gtgcccatca
     7921 ggatgcgaaa tggcgcacct ctcaggcgcg ccgccctttc ctgctggcaa atacccgtaa
     7981 tttagaccgt gaacctatcg gcctgatgga tactgtcgcc acggtcacac cggcacaatt
     8041 gctccaattt tatcaacgct ggtatcaacc aaataatatg acctttatcg tggttggcga
     8101 tatcgacagt aaagaagcgc tggcgctgat aaaggataat ttaagtaagc ttccggctaa
     8161 caaagcagct gaaaatcgcg tctggccgac aaaagccgaa aaccacctgc gctttaatat
     8221 cattaatgat aaagaaaacc gggtgaacgg catcgcactc tattatcgcc tgccaatggt
     8281 acaagtgaac gatgagcaaa gctttatcga acaagctgaa tggagcatgt tagttcagct
     8341 gttcaatcaa cgtctgcagg aacgtataca gtcgggcgag ttgaagacta tttctggcgg
     8401 cactgcgcgc agcgttaaaa ttgcacccga ttatcagtcg ctgtttttcc gtgtaaatgc
     8461 acgagacgat aatatgcagg atgctgcgaa tgcattaatg gaagagttgg caaccattga
     8521 tcagcatggt ttttctgctg aagaactcga tgatgtcaaa tctacccgcc tcacctggct
     8581 gaaaaatgcg gttgatcagc aagctgaacg tgatttacgt atgctgacca gtcgcctggc
     8641 atccagctca ttaaataata cgccgttctt gtcgccggaa gagacatatc aactttcgaa
     8701 acgtctgtgg cagcaaatta ccgtgcaaag tctggcggaa aaatggcagc agttaagaaa
     8761 gaaccaggac gcattttggg agcaaatggt aaacaatgag gttgccgcca aaaaagcatt
     8821 gtctcctgcg gctatcctgg cgctggaaaa ggagtacgcc aacaaaaagc tggcggctta
     8881 catcttccca ggcagaaatt tatcgttaac agtagacgct gacccacagg cggaaattag
     8941 cagcaaagaa acgctggctg agaatctgac atcattaaca ctttccaatg gtgcaagggt
     9001 tattctggca aaatccgcgg gtgaagagca aaagctacaa attattgccg tatcgaataa
     9061 aggcgattta agtttccctg cgcagcaaaa atcacttatc gcgctggcaa ataaagcagt
     9121 tagcggaagc ggcgttggcg aactctcctc ttccagcctg aaacgctgga gtgcggaaaa
     9181 ctcggtaacc atgagcagta aagtcagtgg catgaatacg ttgctctccg ttagcgcgcg
     9241 gactaataac cctgaacctg gttttcagtt gattaaccag cgaatcaccc acagcacgat
     9301 taacgacaat atttgggcat cgctacaaaa tgctcaaatt caggcattga aaacgctcga
     9361 ccagcgtcca gcggagaaat tcgcccagca gatgtatgag acgcgctatg ctgatgaccg
     9421 cacgaaatta ctgcaagaaa atcagattgc acagtttact gccgcagatg cgctggctgc
     9481 cgatcgccag ttgttttcat ctccagcgga tatcacgttt gtcattgtcg gtaatgtcgc
     9541 agaagacaaa ctcgtggcgt taattacgcg ttacttagga tcaatcaaac actctgattc
     9601 gccattagcc gcaggtaaac cattaactcg cgcgacggac aacgcatcgg ttactgtaaa
     9661 ggaacaaaat gaacctgtgg cacaggtttc acagtggaag cgttatgatt cccggacacc
     9721 tgttaatctg gcgacgcgta tggcgctcga tgcttttaac gtcgcactgg caaaagatct
     9781 acgcgttaat attcgtgaac aggcatctgg ggcatacagc gtttcttctc gcctctcggt
     9841 tgatcctcag gctaaagata tcagtcattt gctggctttt acttgtcaac cagaacgaca
     9901 tgatgaactg ttaacgttag cgaatgaagt gatggttaag cgtttgacta aagggatcag
     9961 tgagcaagaa ctgaacgaat accagcaaaa cgttcagcgc agcctcgata tccaacagcg
    10021 tagcgttcaa caattagcga acactattgt aaatagtctt attcaatatg acgatcctgc
    10081 agcatggact gagcaggagc aattgttgaa acaaatgacg gtagagaatg ttaacactgc
    10141 cgttaaacaa tatctttctc atccggtaaa tacttatacc ggagtattat tgccaaaata
    10201 ataacagccc cgtcaacaca tcgttggcgg ggattttagc aatattcgct atttttatgt
    10261 aataatttta taaatgcgtt caaaataata accgcgtact aatagtgata ttttaaggtc
    10321 tgatttttac gtgataattc aggaggcaca gaatacgcat aaaaataaca gcataaaaca
    10381 ccttactacc acccaagagc tccctactat attgtttttc aatgaaaaaa tattattcac
    10441 gcaatatctc accataaata acattaggat tttgttattt aaacacgagt cctttgcact
    10501 tgcttacttt atcgataaat cctacttttt taatgcgatc caatcatttt aaggagttta
    10561 aaatggataa gaagcaagta acggatttaa ggtcggaact actcgattca cgttttggtg
    10621 cgaagtctat ttccactatc gcagaatcaa aacgttttcc gctgcacgaa atgcgcgacg
    10681 atgtcgcatt tcagattatc aatgacgaat tatatcttga tggcaacgct cgtcagaacc
    10741 tggccacttt ctgccagacc tgggacgacg aaaacgtcca caagttgatg gatttatcca
    10801 ttaacaaaaa ctggatcgac aaagaagaat atccgcaatc tgcagccatc gacctgcgtt
    10861 gcgtaaatat ggttgccgat ctgtggcatg cgcctgcgcc gaaaaatggt caggccgttg
    10921 gcaccaacac cattggttct tccgaggcct gtatgctcgg cgggatggcg atgaaatggc
    10981 gttggcgcaa gcgtatggaa gctgcaggca aaccaacgga taaaccaaac ctggtgtgcg
    11041 gcccggtgca aatctgctgg cataaattcg cccgctactg ggatgtggag ctgcgtgaga
    11101 tccctatgcg ccccggtcag ttgtttatgg atccgaaacg catgattgaa gcctgcgacg
    11161 aaaataccat cggcgtggtg ccgactttcg gcgtgaccta caccggtaac tatgagttcc
    11221 cgcaaccgct gcacgatgcg ctggataaat tccaggccga caccggtatc gacatcgaca
    11281 tgcacatcga cgccgccagt ggtggcttcc tggcaccgtt cgtcgccccg gatatcgtct
    11341 gggacttccg cctgccgcgt gtgaaatcga tcagtgcttc aggccacaaa ttcggtctgg
    11401 ctccgctggg ctgcggctgg gttatctggc gtgatgaaga agcgctgccg caggaactgg
    11461 tgttcaacgt tgactacctc ggtggtcaga ttggtacttt cgccatcaac ttctcccgcc
    11521 cggcgggtca ggtaattgca cagtactatg aattcctgcg cctcggtcgt gaaggctata
    11581 ccaaagtaca gaacgcctct taccaggttg ccgcttatct ggcggatgaa atcgccaaac
    11641 tggggccgta tgagttcatc tgtaccggtc gcccggacga aggcattccg gcggtttgct
    11701 tcaaactgaa agatggtgaa gatccgggat acaccctgta tgacctctct gaacgtctgc
    11761 gtctgcgcgg ctggcaggtt ccggccttca ctctcggcgg tgaagccacc gacatcgtgg
    11821 tgatgcgcat tatgtgtcgt cgcggcttcg aaatggactt tgctgaactg ttgctggaag
    11881 actacaaagc ctccctgaaa tatctcagtg atcacccgaa actgcagggt attgcccaac
    11941 agaacagctt taaacatacc tgataacgtt taacggtaac ggtgtcccga aacgaacccg
    12001 tttcgggaca atttccaaag tctgttcact ggcattagca acggaaaata ttgttctgaa
    12061 tacgcttcag aacaaaacag gtgcggttcc gacaggaata ccgttttagg gggataatat
    12121 ggctacatta gtacagacag gtaaagctaa gcagctcaca ttactcggct tttttgccat
    12181 aacggcatcg atggtaatgg ctgtttatga ataccctacc ttcgcaacat cgggcttttc
    12241 attagtcttc ttcctgctat taggcgggat tttatggttt attcccgtgg gactttgtgc
    12301 tgcggaaatg gccaccgtcg acggctggga agaaggtggt gtcttcgcct gggtatcaaa
    12361 tactctgggg ccgagatggg gatttgcagc gatctcattt ggctatctgc aaatcgccat
    12421 tggttttatt ccgatgctct atttcgtgtt aggggcactc tcctacatcc tgaaatggcc
    12481 agcgctgaat gaagacccca ttaccaaaac tattgcagca ctcatcattc tttgggcgct
    12541 ggcattaacg cagtttggtg gcacgaaata cacggcgcga attgctaaag ttggcttctt
    12601 cgccggtatc ctgttacctg catttatttt gattgcatta gcggctattt atctgcactc
    12661 cggtgccccc gttgctatcg aaatggattc gaagaccttc ttccctgact tctctaaagt
    12721 gggcaccctg gttgtatttg ttgccttcat tttgagttat atgggcgtag aagcttccgc
    12781 aacccacgtc aatgaaatga gtaacccagg gcgcgactat ccactggcta tgttactgct
    12841 gatggtggcg gcaatctgct taagctctgt tggcggtttg tctattgcga tggtcattcc
    12901 gggtaatgaa atcaacctct ccgcaggggt aatgcaaacc tttaccgttc tgatgtccca
    12961 tgtggcaccg gaaattgagt ggacggttcg cgtgatctcc gcactgctgt tgctgggtgt
    13021 tctggcggaa atcgcctcct ggattgttgg tccttctcgc gggatgtatg tcacagcgca
    13081 gaaaaacctg ctgccagcgg cattcgctaa aatgaacaaa aatggcgtac cggtaacgct
    13141 ggtcatttcg cagctggtga ttacttctat cgcgttgatc atcctcacca ataccggtgg
    13201 cggtaacaac atgtccttcc tgatcgcact ggcgctgacg gtggtgattt atctgtgtgc
    13261 ttatttcatg ctgtttattg gctacattgt gttggttctt aaacatcctg acttaaaacg
    13321 cacatttaat atccctggtg gtaaaggggt gaaactggtc gtggcaattg tcggtctgct
    13381 gacttcaatt atggcgttta ttgtttcctt cctgccgccg gataacatcc agggtgactc
    13441 taccgatatg tatgttgaat tactggtcgt tagtttcctg gtggtacttg ccctgccctt
    13501 tattctctat gctgttcatg atcgtaaagg caaagcgaat accggcgtca ctctggagcc
    13561 aatcaacagt cagaacgcac caaaaggtca cttcttcctg cacccgcgtg cacgttcacc
    13621 acactatatt gtgatgaatg acaagaaaca ctaactatgt ctgaatggtt ataagataag
    13681 ggagcgataa ttcatcgctc cctttttcgt gcttgctgtc gtattgactt aaccgggata
    13741 agtacgagaa tgagcgcaca tctgtttacc ggaaactagc acatatggat atctgctccc
    13801 gaaacaagaa attaacgatt agaagaccag cgatactggt tgcactggca cttttactgt
    13861 gtagttgtaa aagcacgcct ccagagtcca tggtgacacc accagcgggt tcaaagccac
    13921 cagccacgac gcaacaatcg tcacaaccga tgcgtggcat ctggctggcc acggtgtctc
    13981 gcctcgactg gccaccggtt tcctcggtta acattagtaa ccccaccagc cgggcccgtg
    14041 tacaacaaca ggcgatgatc gacaaactgg atcatttgca acgtctcggt ataaacacgg
    14101 tctttttcca ggtcaagccg gacggtaccg ccctgtggcc atcgaaaatt ttgccgtggt
    14161 ccgatcttat gaccggtaag attggtgaaa atccgggtta cgatccgctg caatttatgc
    14221 tcgacgaagc ccacaagcgt gggatgaaag tacacgcctg gtttaacccc tatcgcgtat
    14281 cggttaatac gaagcccggt actatcaggg aactgaatag cactctgtct caacaaccgg
    14341 cgagcgtcta tgtgcaacac cgtgactgga tcagaacgtc tggcgatcgc tttgtcctcg
    14401 acccgggcat ccctgaggtt caggactgga tcacatcaat agtcgcagaa gtggtttccc
    14461 gctatccggt agatggcgtg cagtttgacg actatttcta tactgagtca ccgggttcac
    14521 ggctaaatga taacgaaacg taccgtaaat acggaggcgc atttgcgtca aaagcagact
    14581 ggcggcgcaa caatactcag cagttaattg caaaggtatc gcacaccatt aaaagcatta
    14641 agccgggagt cgaatttggt gttagcccgg caggcgtgtg gcgtaaccga tcacacgatc
    14701 cgctcggttc cgatacccga ggcgcggcag cctatgacga atcctacgct gacactcgtc
    14761 gatgggtgga acaaggattg ctggattaca ttgctcccca aatttactgg ccgttctcac
    14821 ggagtgccgc gcgttatgac gtgttggcaa aatggtgggc ggatgtcgtt aaaccgacca
    14881 ggacccgcct gtatatcggt atcgccttct ataaagtggg tgaaccttca aagatagagc
    14941 cagactggat gattaacggc ggcgtaccgg agctgaaaaa gcagctcgat cttaacgatg
    15001 cggtaccaga aattagcggc accatcttgt tccgtgagga ctatctgaat aaaccgcaga
    15061 ctcaacaagc ggtcagctat ctgcaaagtc gttggggcag ttaaactccc attcttactt
    15121 tgtaacaagc cggtgcttta cccactggct tgttaccctc ttgaactatc ccaccagcaa
    15181 tcatgcttac taaagtcaaa ttaaccacgc aattaaccac gcttagttaa acagccataa
    15241 atcccctaat aaaaatgtat gaatatgaaa aaccatacga tattcgcagt caccgaaaaa
    15301 taattacgat gggctatact cttctgaaga acagtagcgt agattgaaac tgtcctaagt
    15361 cgcttatttc ttttcagtat atcttcatat ttcaggagaa tatggtgcag ttagtcgatc
    15421 tcgcacgtcg cgtttcattt tgattccgtc tgatttccca gccttataag ggtgtgaatt
    15481 atggagatgt attttaaaag aatgaaagat gagtggaccg gacttgtcga gcaggcagat
    15541 ccgcccattc gtgctaaagc cgcggaaatt gccgttgcgc atgctcatta tctgagtatc
    15601 gagttttatc gaattgtccg catcgacccg catgccgaag aattcttgag taatgaacaa
    15661 gttgagcggc agttgaaaag tgcgatggaa cgctggatta ttaacgtgct ttctgcccag
    15721 gttgacgatg tcgaaaggct aatacaaatc cagcataccg tcgcggaagt gcatgcccgc
    15781 ataggaattc cggtagaaat tgtcgagatg gggtttcggg tgctgaaaaa gatcctctat
    15841 ccggtaatct tctcttcgga ttattctgcc gcagaaaaac ttcaggtcta ccatttctcg
    15901 attaacagta ttgatatcgc tatggaagtg atgacccgcg cgtttacctt tagtgacagt
    15961 agtgcctcaa aagaagatga aaactatcgt atcttctcgt tactggaaaa cgccgaagaa
    16021 gaaaaagaac ggcaaatagc ctcaatactt tcatgggaaa tagatattat ctataaaatc
    16081 ctgctggatt ctgatttagg cagtagtttg cctttaagcc aggctgattt tggcctgtgg
    16141 tttaaccata aaggtcgaca ttatttcagc ggcattgctg aagtgggcca tatctctcgt
    16201 ctgattcagg atttcgacgg tattttcaat caaaccatgc gtaacaccag gaatttgaat
    16261 aacagaagtc tgcgggtgaa atttttatta cagataagaa ataccgtatc gcaaattatt
    16321 accttgctgc gtgaattgtt tgaagaagta tcgcgccacg aagtcggtat ggatgtactg
    16381 acgaaattac ttaaccgccg tttcctgccg actatcttca aacgcgaaat tgcccatgcc
    16441 aaccggaccg gtacaccgct gtcagtgctg attattgacg ttgataaatt caaagagatc
    16501 aacgatacgt ggggccataa cactggcgat gaaattctgc gtaaagtctc tcaggccttt
    16561 tatgacaacg tccgcagtag tgattatgtt ttccgctacg ggggcgatga atttatcatt
    16621 gttttgactg aagcttctga aaacgaaacg ttacgtaccg cagaacgtat tcgcagtcgg
    16681 gtggagaaaa ccaaactgaa agccgcaaac ggcgaagata ttgccctctc actttccatc
    16741 ggtgccgcca tgtttaatgg tcatcctgac tatgagcgcc tcattcaaat agccgatgaa
    16801 gctctgtata tcgccaaaag acgaggtaga aaccgtgttg aactctggaa agccagtctt
    16861 tagatgcgcc aggatgcaga ggtaatcatg aagctaaccg atgcggataa tgccgccgat
    16921 ggcatttttt tccccgccct tgagcaaaat atgatgggtg cggtgttaat taacgaaaat
    16981 gatgaagtga tgtttttcaa ccccgccgca gagaagctct gggggtacaa acgtgaagaa
    17041 gtcattggca ataacattga tatgctgatt ccgcgggatt tgcgtcctgc gcatcctgaa
    17101 tacattcgtc acaaccgtga aggcggtaaa gcgcgcgtcg aggggatgag tcgggagctg
    17161 cagctggaga aaaaagacgg cagtaaaatc tggacccgtt ttgcgctatc gaaagtgagc
    17221 gccgagggga aagtttatta cctggcgctg gtacgggatg ccagcgtaga aatggcgcaa
    17281 aaagaacaga ccaggcaatt gattattgcc gttgaccatc tcgaccgacc ggtgattgtc
    17341 ctcgatccgg aacgccatat tgtgcagtgc aatcgcgcat ttaccgaaat gtttggttac
    17401 tgcattagcg aagccagcgg tatgcagcct gatacactcc tgaacattcc tgaattccct
    17461 gccgataacc gcattcgttt acaacagttg ctttggaaaa ccgcccgcga tcaggacgaa
    17521 tttctgctgt tgacgcgcac cggtgaaaaa atctggatta aagcttctat cagcccggtt
    17581 tatgacgtgc tcgcgcatct gcagaacctg gtaatgacct tctcggatat caccgaagaa
    17641 cggcaaatcc gccagcttga aggcaatatt ctcgccgcca tgtgcagcag cccgccattt
    17701 catgaaatgg gggaaatcat ttgtcgtaac atcgaatctg ttctcaacga atcgcatgtt
    17761 tcgctgttcg cactgcgcaa cgggatgccg atacactggg cgtcatcttc gcacggtgca
    17821 gaagttcaaa atgcgcaaag ctggtcagcg accattcgtc agcgtgatgg cgcgcctgcg
    17881 gggatcctgc aaattaaaac ctcgtcagga gcagaaacca gcgcctttat cgaacgcgtg
    17941 gcagatatca gccagcatat ggccgcgctg gcgctggaac aggaaaaaag ccgtcagcat
    18001 attgaacaac tcatccaatt tgatccgatg accggtctgc caaatcgcaa taacctgcac
    18061 aattacctcg atgacctggt cgacaaagcc gtctctccag tatcggtgtt gaccatattc
    18121 aggatgtgat tgatagcctt ggctatgcgt gggccgatca ggcattgctg gaagtggtca
    18181 atcgctttcg tgaaaaactc aaaccggatc agtatctctg tcgtatcgaa ggtacgcagt
    18241 ttgtcctcgt gagcctggaa aacgacgtca gtaacattac ccagatcgcc gatgagctac
    18301 ggaatgtggt cagcaagccg ataatgattg acgataaccc cttcccgctt accttgagta
    18361 ttggcatcag ctacgacgtg ggtaaaaatc gcgattacct gctctccact gcccataacg
    18421 cgatggatta tattcgcaag aatggcggta acggctggca gttcttcagc ccggcgatga
    18481 acgaaatggt aaaagagcgt ttggttttag gcgcagcgct gaaagaagcg attagcaata
    18541 accaactgaa actggtttac cagccgcaaa tcttcgcaga aacgggtgaa ctctacggca
    18601 tcgaagccct tgctcgctgg cacgatcccc agcatggtca tgtgccccct tcacggttta
    18661 ttcctctcgc agaagagatt ggtgaaatcg aaaatattgg gcgctgggtc atcgcggaag
    18721 cttgccgtca gttagcagaa tggcgtagcc agaatattca tatcccggcg ttatccgtga
    18781 acttgtcggc gctgcacttt cgcagtaatc agctgcctaa tcaggtgtct gatgcaatgc
    18841 aagcctgggg tattgacggc caccagctga cggtagaaat cacggaaagc atgatgatgg
    18901 aacacgatac cgaaatcttt aagcgcattc agatcctgcg tgatatgggc gtgggtttat
    18961 cggtagatga ttttggcacg ggcttttccg gattatcccg cttagtcagt cttccggtaa
    19021 cggaaatcaa aattgacaaa agttttgtcg atcgttgtct gacggaaaaa cgcatcctgg
    19081 ccttacttga agccattacc agcattgggc aaagcctcaa tttaaccgtc gtggcggaag
    19141 gcgtcgaaac caaagagcaa tttgagatgc tacgaaagat ccactgtcgc gttattcagg
    19201 gatatttctt ttcccgcccc ctacccgccg aagaaattcc aggctggatg agcagcgtgt
    19261 taccgctgaa aatctgacaa attcctctct cccgcactcg cgggttttca tttaacgtga
    19321 cactgtcact taaatactgt gatttcagct acgattccgg gagattcctt ccttaacttc
    19381 ccctcttgca ccaaaactgc actacgctgc acaaatgttg atcaaaagtt aaaaaatata
    19441 actttaagcc agcattctgg cgcttatccc ggcatggcat gagatctgca taagcggaaa
    19501 gcgcagcaat ttttgtctta tacaggaacc gtttatgtcg gataccaccg aactggttga
    19561 tttagccgtg atcttccctg atctggagat cgaattgaaa tacgcctgcg ctgataacat
    19621 cacaggtaaa gctatttacc agcaagcgcg ttgtctgtta cacaaggatg cgattaccgc
    19681 gctggcgaaa agtatcagta tcgcccagct atcaggctta caactggtga tttacgatgc
    19741 gtatcgccca caacaagcac aggcgatgtt gtggcaagcc tgcccagacc cgcaatatgt
    19801 tgttgatgtg acggtcggtt ctaatcacag ccgtggcacg gcgatcgacc tgacgcttcg
    19861 tgatgagcac gggaacatcc tggatatggg cgcagggttc gatgaaatgc acgagcgctc
    19921 ccatgcctat cacccttccg tcccgcccgc cgctcagcgc aatcgcctgt tgctgaatgc
    19981 gataatgact ggtggcggtt ttgtcggtat ctccagcgaa tggtggcact tcgaattacc
    20041 ccaggcagcg agttaccctc tgctcgctga tcaattcacc tgttttatat cccctggcac
    20101 acagcacgtc agttaatcag gaaactgcca tgaagagatc gatatcgttt cgtcccacat
    20161 tgctcgcgct cgtccttgcc acaactttcc cggttgcgca cgccgccgta ccgaaagata
    20221 tgctggtgat tggtaaggcc gccgatccac aaaccctcga cccggcggta acaatagata
    20281 ataacgactg gacagtgacc tacccgtctt atcagcgact ggttcagtac aaaacggacg
    20341 gtgataaagg ctcaaccgac gttgaaggcg atctggcaag tagctggaaa gcgtctaacg
    20401 atcaaaaaga gtggacgttc accctgaaag ataatgctaa atttgccgat ggcacacctg
    20461 tcactgccga agcagtaaaa ctctcttttg agcggttact aaaaatcggc caggggccag
    20521 cagaagcatt tcccaaagat ttaaagattg atgctcccga cgaacatacg gtgaagttta
    20581 cccttagcca gccatttgca ccgttcctct acacgctggc gaatgacggt gcatccatta
    20641 tcaatccggc ggtattaaag gaacatgcgg cggatgatgc ccgcggcttc ctcgcgcaaa
    20701 ataccgccgg ttccggacca tttatgctga aaagctggca aaaaggtcag caattagttc
    20761 tggtgccaaa tccgcattac cccggcaata aaccgaactt taagcgagta tcggtaaaaa
    20821 ttatcggtga aagtgcctcc cgtcgcctgc agctctcccg tggcgacatt gacattgccg
    20881 atgcgctgcc ggtggatcaa ctcaacgccc tgaagcagga aaacaaagtc aatgtggcag
    20941 agtatccgtc actgcgcgtc acctatctgt atctgaataa cagcaaagcg ccacttaatc
    21001 aggcggatct gcggcgggcc atttcctggt ctaccgatta tcagggcatg gttaacggca
    21061 ttctgagtgg taacggaaaa caaatgcgcg gcccgattcc ggaaggcatg tggggctacg
    21121 atgcgacggc aatgcaatac aaccatgacg aaacgaaagc caaagccgaa tgggataaag
    21181 tgacgagcaa acccaccagc ctgacgtttc tctactccga taacgatccg aactgggaac
    21241 cgatcgctct ggcaacacaa tccagcctca acaagctggg catcaatgtg aagctggaaa
    21301 agctggcgaa cgccaccatg cgcgacagag tgggtaaagg tgattacgac attgcgattg
    21361 gcaactggag tccggatttt gccgacccgt atatgtttat gaattactgg tttgagtcag
    21421 acaaaaaagg tctgccgggt aaccgctcgt tctatgaaaa cagtgaggtc gataagttac
    21481 tgcgcaatgc gcttgcgacc accgaccaga cgcagcgtac ccgagactac cagcaggcac
    21541 agaaaatcgt cattgatgac gctgcttatg tgtacctgtt ccagaaaaac taccaactgg
    21601 cgatgaacaa agaggtgaaa ggctttgtgt tcaatcccat gctggaacag gtcttcaata
    21661 tcaataccat gagtaaataa cgtgaccttc tggagtattt tacgccaacg ctgctggggg
    21721 ctggtgctcg tggtggcggg cgtctgcgtg attacgttta ttatctcgca tctgatcccc
    21781 ggcgatccgg cgcggttact ggcgggtgac cgcgccagcg atgctatcgt ggaaaatatt
    21841 cgccagcaac tgggactgga ccagccactg tatgtacagt tttaccgcta cgtcagcgac
    21901 ctgtttcatg gtgacctggg aacatccatt cgtactgggc gtccggtgct ggaagagttg
    21961 cgtatatttt tcccggcgac gctggagctg gctttttgtg ccctgctgct ggcactcttg
    22021 attggcatcc cgcggggcat actctctgca gtctggcgaa atcgctggct ggatcatctg
    22081 gtgcgaataa tggcgattac cggaatctcc acacctgcgt tctggcttgg gctgggcgtc
    22141 atcgtgctgt tttatggtca tctgcaaata cttcccggcg gcggaaggct tgatgactgg
    22201 ctggatccac caacgcacgt taccggcttt tatctgctcg atgcgttgct tgaaggcaac
    22261 ggtgaagtct tcttcaatgc gttgcaacat ctcatcttac cggcattaac gctggcgttt
    22321 gttcacctgg ggattgtcgc ccgccagatc cgctcagcga tgctggaaca attgagtgaa
    22381 gactacattc gtaccgcccg ggccagcggc ttgcccggct ggtatatcgt tctatgttat
    22441 gcgctaccca atgcgttgat cccttcgatt accgtattgg ggctggcgct gggcgatttg
    22501 ttgtatggcg cagtgctcac cgaaaccgtt tttgcctggc ccggaatggg ggcatgggta
    22561 gtgacatcaa tacaggcgct cgacttcccg gcagtgatgg gctttgccgt cgtggtttca
    22621 tttgcttatg tgctggtcaa cctggtggtg gatttgctct atttgtggat tgatccgcgt
    22681 atcggacgtg gaggtggtga atgatgctaa gcgaggaaac gtccgccgta cgcccacaaa
    22741 aacaaacgcg atttaacggt gcaaaactgg tatggatgct gaaaggcagt ccgctcaccg
    22801 tgaccggcgc agtcatcatt gtattaatgc tactgatgat gattttttca ccgtggctgg
    22861 cgacgcatga tcccaacgcc attgatttaa ccgcccgcct tttgccgcct tctgcggcgc
    22921 actggtttgg caccgatgaa gtgggacgcg atctgttcag ccgcgtactg gtcggcagtc
    22981 agcaatcaat tctcgccgga ttagtcgtgg ttgccattgc gggtatgatt ggttcgctac
    23041 tcggatgtct atccggtgtg cttggcggac gcgcagacgc catcatcatg cgcatcatgg
    23101 acattatgct gtcgattcct tcgttggtac tgacaatggc actggctgcc gctctcgggc
    23161 cgagtttgtt taacgccatg ctggctattg ctattgtgcg aattcccttt tatgtgcgcc
    23221 tggcgcgggg gcaagcatta gttgtacgcc agtataccta tgttcaggcg gcgaaaacct
    23281 ttggcgcgtc tcgttggcat ctgatcaact ggcatatttt acgtaactcc ctaccgccgc
    23341 tgatcgtgca ggcatcgctg gatatcggta gcgcgatttt aatggccgcc acgttgggat
    23401 ttattggcct gggtgctcaa caaccgagtg ctgaatgggg cgcgatggta gcaaatggtc
    23461 gcaactatgt actcgatcag tggtggtatt gcgcatttcc gggggcagcg attttgctta
    23521 ccgccgtcgg gtttaatctc tttggtgatg gtattcgcga tctgcttgac ccgaaagcag
    23581 gaggaaagca gtcatgaccc aacccgttct ggacattcaa caactgcatt tgagtttccc
    23641 cggttttaac ggcgacgttc acgcgctcaa caatgtgtcc ttgcagatta accgcggtga
    23701 aattgtcggt ctggtgggag aatccggctc aggtaaatca gtcaccgcaa tgctgattat
    23761 gcgtctgcta ccgacgggca gttattgcgt acatcggggg cagatttcac tgctgggaga
    23821 cgatgttctt aacgcgcggg aaaaacagct tcgtcagtgg cgcggcgcac gagtggcgat
    23881 gatctttcag gaaccgatga ccgccctcaa tccgacacgt cgaataggtc ttcagatgat
    23941 ggacgtgatc cgccatcatc aaccaataag tcgtcgggaa gccagagcta aagcgattgc
    24001 cctgctggaa gagatgcaaa tcccggatac cgtggaagtt atgtcgcgct atccgtttga
    24061 gctttcaggt ggtatgcgcc agcgggtaat gattgcgctg gcattctcct gcgaaccgca
    24121 attgattatt gccgacgaac cgactacggc gctggacgtc acggtacagt tgcaggtact
    24181 gcgtttgctt aaacataaag ctcgcgacag tggaactgcg gtactgttca tcagccatga
    24241 tatggccgtg gtgtcgcaac tgtgcgatag cgtttacgtg atgtatgccg gaagcgtgat
    24301 tgagagcggc gtgacggcag acgttatcca tcatccccgg catccgtata ccattggttt
    24361 gctgcaatgc gcaccagaac atggagtacc acgccagcca ttacccgcca ttccagggac
    24421 ggtaccaaat ctcacccatt tgcctgacgg ctgcgctttt cgcgatcgtt gctatgcggc
    24481 aggtgcacag tgtgaaaacg tcccggcgct gacagcgtgt ggtgacaaca accagcgctg
    24541 cgcctgttgg tatccgcagc aggaggtcat aagtgtctga cacgttatta acgttacgcg
    24601 acgtccatat caatttcccg gcgcgtaaaa actggcttgg taaaactacg gaacatgttc
    24661 atgccattaa tggtattgat ttacagatcc gccgtggtga aaccttaggg atcgtcggcg
    24721 agtcaggctg cggcaaaagc accctcgcac agcttttaat gggtatgctg caaccgagcc
    24781 acgggcagta catccgttct ggctcacaac gcattatgca gatgatgttt caggacccgc
    24841 tctcttcgct taatccgcgc ttaccggtgt ggcgcatcat cacagaaccg ctctggatag
    24901 ctaagcatag tagtgaacaa cagcggcgag cgttggcaga ggagctggct gtgcaggtgg
    24961 gtattcgtcc ggagtatctc gaccgcctgc ctcatgcgtt ctccggcggg cagcggcaac
    25021 gcatcgccat tgccagagca ctctcttcgc agcctgacgt gattgtgctt gatgagccaa
    25081 cctctgcgct ggatatctcc gtgcaggcgc agatcctcaa tttactggta acgctacagg
    25141 aaaatcacgg gctgacttat gtgctgattt cacacaatgt ctcggtgata cgtcatatga
    25201 gcgatcgggt ggcggtgatg tatctcgggc agattgtgga attaggggaa gcgcaacagg
    25261 tgctaacggc tccggcacat ccatacaccc gattattgct ggattccctc cccgccattg
    25321 ataaaccact ggaggaagaa tgggcattac gtaaaacgga tctgccagga aaccgcacgt
    25381 tgccacaggg ctgttttttc cgcgaacgtt gcccccttgc aaccagcgga tgcgaagtcc
    25441 ggcaatcatt aacgacgcgg gctgacggac gtgagatccg ttgctggcgg gcgctgtaga
    25501 gatgctaaca acgcatcagg cattcatgcg cctgacgcgt catccggcaa tgctttacga
    25561 tttcaactgg taatccagcg taatttccgc tttcagtacc tgagagaccg ggcatcctgc
    25621 ttttgctttc tggatgatgc cgtcaaaggt agaggcatca atacccggca ccgcaacttc
    25681 actcttcagt gcgattttcg taatcgcaaa accggcatcc actttatcca gcgacacatc
    25741 ggcggtggta tcaatcgatg ttggcgtgaa tcccgcttcc cccagcatta atgaaagcgc
    25801 cattgagaaa catgcggcat gcgctgcgcc aatcagttct tcagggttgg ttcctttttc
    25861 gccttcaaaa cgcgtgttaa atccatacgg ctgttggttc agcacgccac tctcggtgga
    25921 tactgttccc ttcccgcgtt tgatatcgct ttcccagtgt gcctgacctt tcttatggat
    25981 tgtcattgtt gctctcctgt gggcttaaaa aggaaattat agactacgtg agaaaccgca
    26041 cgaggataag caggatattc cgaataaaat acggcaaaaa tattacttct gcacttaaat
    26101 taaattccca caatagataa ccggaaatat atatctcaag agagattgca tcaatattaa
    26161 tcaactcatc acaataataa tacctatcaa tatcatgggg ttaaaatata aaaatcatca
    26221 accaggacta atcttaacaa cgaagcggca aatatttgcc atgctgaatg tgctttataa
    26281 atctgcgatc cgtagcagac actataaata cacagacacg gagaatcact atgtttactt
    26341 attatcaggc agaaaattca acagcagaac ccgctctggt taatgccatt gagcaaggcc
    26401 ttcgggcaga gcacggtgtt gtcactgaag atgacattct catggagctg accaaatggg
    26461 tggaagcttc tgataacgac atcctcagtg atatctacca gcaaacgatc aattatgtgg
    26521 tcagtggcca gcaccctacg ctttaagatg ctatgcttga tcggcaacct aatttagggg
    26581 tttagcacgt gtttcttcgc tacggcgatg ttgtccttaa aactagcaac aggattgagg
    26641 agttaaaatg aaatcgaacc gtcaggcacg tcatattctt ggactggacc ataaaatatc
    26701 taaccagcgc aaaatagtta ccgaaggtga caaatccagc gtggtaaata acccaaccgg
    26761 cagaaaacgc cccgctgaaa agtaattcat aaccatcagt cctcaatgac gattaaacac
    26821 cattgcctgc gcaatggtgt ttttgttttt atctgcttta tacttggggc cgacgccctg
    26881 gcggtaaagc aaagacgata aaagcccccc agggatggat attcaaaaaa gagtgagtga
    26941 catggaacca aaaacaaaaa aacagcgttc gctttatatc ccttacgctg gccctgtact
    27001 gctggaattt ccgttgttga ataaaggcag tgccttcagc atggaagaac gccgtaactt
    27061 caacctgctg gggttactgc cggaagtggt cgaaaccatc gaagaacaag cggaacgagc
    27121 atggatccag tatcagggat tcaaaaccga aatcgacaaa cacatctacc tgcgtaacat
    27181 ccaggacacc aacgaaaccc tcttctaccg tctggtaaac aatcatcttg atgagatgat
    27241 gcctgttatt tataccccaa ccgtcggcgc agcctgtgag cgtttttctg agatctaccg
    27301 ccgttcacgc ggcgtgttta tctcttacca gaaccgccac aatatggacg atattctgca
    27361 aaacgtgccg aaccataata ttaaagtgat tgtggtgact gacggtgaac gtattctggg
    27421 gcttggtgac cagggcatcg gcgggatggg cattccgatc ggtaaactgt cgctctatac
    27481 cgcctgtggc ggcatcagcc cggcgtatac ccttccggtg gtgctggatg tcggaacgaa
    27541 caaccaacag ctgcttaacg atccgctgta tatgggctgg cgtaatccgc gtatcactga
    27601 cgacgaatac tatgaattcg ttgatgaatt tatccaggct gtgaaacaac gctggccaga
    27661 cgtgctgttg cagtttgaag actttgctca aaaaaatgcg atgccgttac ttaaccgcta
    27721 tcgcaatgaa atttgttcct ttaacgatga cattcagggc accgcggcgg taacagtcgg
    27781 caccctgatc gcagcaagcc gcgcggcagg tggtcagtta agcgagaaaa aaatcgtctt
    27841 ccttggcgca ggttcagcgg gatgcggcat tgccgaaatg atcatcgccc agacccagcg
    27901 cgaaggatta agcgaggaag cggcgcggca gaaagtcttt atggtcgatc gctttggcct
    27961 gctgaccgac aagatgccga acctgctgcc tttccagacc aaactggtgc agaagcgcga
    28021 aaacctcagt gacagggata ccgacagcga tgtgctgtca ctgctggatg tggtgcgcaa
    28081 tgtaaaacca gatattctga tcggcgtctc aggacagacc gggctgttta cggaagagat
    28141 catccgtgag atgcataaac actgtccgcg tccgatcgtg atgccgctgt ccaacccgac
    28201 gtcacgcgtg gaagccacac cgcaggacat tatcgcctgg actgaaggta acgcgctggt
    28261 cgccacgggc agtccgttta atccagtggt atggaaagat aaaatctacc ctatcgccca
    28321 gtgtaacaac gcctttattt tcccgggcat cgggctgggt gttattgctt ccggcgcgtc
    28381 acgtatcacc gatgagatgc tgatgtcggc aagtgaaacg cttgcccagt attcgccgct
    28441 ggtcctgaac ggcgaaggtc tggtactacc ggaactaaaa gatattcaga aagtctcccg
    28501 cgcaattgcg tttgcggttg gcaaaatggc gcagcagcaa ggcgtggcgg tgaaaacgtc
    28561 tgccgaagct ttgcaacaag ccattgacga taatttctgg caagccgaat accgcgacta
    28621 ccgccgtacc tccatctaag cctgcgcccg gtagtgaagg ctaccgggct atttccctct
    28681 ccctttttca gatctcatcc atactgggta gtggcgaata aatctcattt gcctcacctg
    28741 ctatgcagaa catcatccga aaaggaggaa ctatgaaggc tgcagttgtt acgaaggatc
    28801 atcatgttga cgttacggat aaaacactgc gctcactgaa acatggcgaa gccctgctga
    28861 aaatggagtg ttgtggtgta tgtcataccg atcttcatgt taagaatggc gattttggtg
    28921 acaaaaccgg cgtaattctg ggccatgaag ggattggtgt ggtggcagaa gtgggtccag
    28981 gtgtcacctc attaaaacca ggcgatcgtg ccagcgtggc gtggttctac gaaggatgcg
    29041 gtcattgcga atactgtaac agtggtaacg aaacgctctg ccgttcagtt aaaaatgccg
    29101 gatacagcgt tgatggcggg atggcggaag agtgcatcgt ggtcgccgat tacgcggtaa
    29161 aagtgccaga tggtctggac tcggcggcgg ccagcagcat tacctgtgcg ggggtcacca
    29221 cctacaaagc cgttaagctg tcaaaaattc gtcccgggca gtggattgct atctacggtc
    29281 ttggcggtct gggtaacctc gccctgcaat acgcgaagaa tgtctttaac gcgaaagtga
    29341 tcgccattga tgtcaatgat gagcagttaa aactggcaac cgaaatgggt gcagatttag
    29401 cgattaactc acgcaccgaa gacgccgcca aaattgtgca ggagaaaacc ggtggcgctc
    29461 acgctgcggt ggtaacagca gtagctaaag ctgcgtttaa ctcggcagtt gatgctgtcc
    29521 gtgcaggcgg tcgtgttgtg gctgtcggtc tgccgccgga gtctatgagc ctggatatcc
    29581 cacgtcttgt gctggatggc attgaggtgg tcggttcgct ggtcggcacg cgccaggatc
    29641 taactgaagc cttccagttt gccgccgaag gtaaagtggt gccgaaagtc gccctgcgtc
    29701 cgttagcgga catcaacacc atctttaccg agatggaaga aggcaaaatc cgtggccgta
    29761 tggtgattga tttccgccgc taagaggcct ttgctgcgac tcccatgttc gggtcgcagc
    29821 atcgcacact ctccaacatg aaatggctaa atggattatt gttgcgtagg aaggaacaat
    29881 aatgatagtg ttcccagaga aggaacgcta ctgagagtaa agacactaat gaaaatggcc
    29941 aatcatcccc gcccggggga cattattcag gaatcactgg acgaacttaa tgtcagcctg
    30001 cgcgagtttg ccagagcaat ggaaattgct ccctcaacgg caagccgatt gctgaccgga
    30061 aaagcagctt tgacgccaga aatggcaatt aaactttccg tggtgatcgg cagttcgccg
    30121 caaatgtggc tgaatctgca aaacgcctgg agtctggcag aggctgcaaa aacggtggat
    30181 gtgtcgcgat tacgccgtct ggttgcgcaa taaaaaatgg cgccttttac agcgccattt
    30241 gaaactgatg acaaacgcaa cattgcctga tgcgctacgc ttatcaggcc tacacgttca
    30301 ctgcaatcta ttggatttga acggtttgta ggccggataa ggcgttcacg ccgcatccgg
    30361 catgaacaaa gcctacgttg tctacaatat gaaaatggcg ccttttacag cgccattttc
    30421 agcttaaagt tcaaaagaga ttatatccct tcttcactct cttttttcgc ttctgccttc
    30481 tcgatttcac gataccagcg cggatggtgt ttcttcgccc agcgacggct caccttccct
    30541 tcgatcatcc ctttaatcga tcctttcacc cagaatgcca tatacatatg gatcaggatg
    30601 gcgtggatca ggatgatacc cgcagccgcg tggatcagca ggctgtagcg aacaacctgc
    30661 atcgggaagt actgcgcaaa gtacggacgc cagataatca ccccggtcac cagcagcacg
    30721 aaaatcatgc tcatgatcga ccagaacatc atcttttgcc cggcgttgta cttaccgaca
    30781 tccgccactt tatgctcatt gcctttcaat acttcgacaa tgtttaacag ccacggaata
    30841 tctttcttat ccgggatgtt gtgatgcaca aaacgcacaa acataaacat cagtgcgacg
    30901 aaaatcgcaa tgccgaagaa tgggtgcaaa atgcgtccca tctgcggcgt accgaaggtt
    30961 tgcgtcagcc attgcagcgt cgggaagaag aacgaaatcc cggacagcgc caccaggaag
    31021 aagcaaatca ccacggtcca gtgacaggcg cgatcaataa atttggtgcg cacaatcatt
    31081 ttcgacttac tcatgatgat cctcctcgtc atcgtccact tccttattcg ggccaatacc
    31141 gatgtagtgg aaaatcaacc ctgcaaaagt ggcaataaag ccagccgctg ccagcggttt
    31201 caacgcgcct ttccacaggc ttaccgaggt gtcgatcttc ggatctttcg gcagaccgtg
    31261 atacagctcc ggctgatcgg cgtgatgcag cacgtacata acgtgcgtac caccgacccc
    31321 ttccgggttg tagacgccag catgttcgta accacgcgct ttcagtttcg ccacgcgctg
    31381 ttccgccagc tccagcatct ccttcttggt gccgaagtgg atagccccgg tcggacaggt
    31441 tttcacacaa gccggttcct ggccgacgct gacgcgatcg acgcagagcg tgcatttata
    31501 tacccggtta tcctctttgt tgaggcgcgg aatattaaac ggacacccgg caatgcagta
    31561 accacagccg atgcagtttt ccgactggaa atcgacaatc ccgttagcgt actgaatgat
    31621 tgcaccggca gacgggcacg ccttcaggca gcccggatct tcacagtgca tacagccatc
    31681 tttacggatc agccactcca gcttgccgct ctgttcggtt tcgctaaagc gcatcaccgt
    31741 ccaggacttg gcgctcagat cggcggggtt atcgtaaacc ccgacgcagt gccccacttc
    31801 atcacggatg tcgttccact ccgaacacgc cacctgacag gctttacagc cgatacaggt
    31861 ggaaacgtcg ataagttttg cgacttctgc tttgtaatca cgcacctgag aaggcggcgt
    31921 gatggagtta gttgcggacc ttttgataat gtcctgcgtt tccatagcca tctgttcgcc
    31981 cccttacgcc ttctcgatgt tgactaagaa cgctttatat tccggcgttt gcgagtttgc
    32041 atcaccgaca ttcggcgtca gagtgttagc tatataacct ttacgcgcga caccctcaaa
    32101 gccccagtgg attggaatac ccaccgtttc aacctgctga ccatttacat tcagcggttt
    32161 cagacgacgc gttaccacag ccaccgcgcg gataaagcca cgcttgcttg agacagtgac
    32221 acgatcgcca ttattaatgc ctttcgccgc cgccagcgtt tcgctgattt ccacaaactg
    32281 ttccggctga gcaattgcgt tgagcaacgc gtgcttggtc caggtgtgga agtgctcggt
    32341 cagacgatag gtcgtaccca catacgggaa ctgctctttt ttacccatcc gcagcgcgtc
    32401 ttgttcatac agacgaacca ccgggttaga aaccacgttc ggatgcagcg ggttagttcc
    32461 cagcggcgtt tcaattggct cgtagtgttc cgggaacgga ccttccgcca ttttgttgat
    32521 ggcaaacagg cgtcccatcc cttccggctg catgataaac ggcccggttg gcgtacccgg
    32581 tgcggcattg ccgaagtcag ggatatcgtt acccgtccac ttgctgccgt tccactggat
    32641 cagcatccgt ttcggatccc acggtttacc gttgatatct gccgaagcac ggttgtagag
    32701 tacgcgacgg ttaagcggcc acgcccaggc ccatcccagc gtattcccca gaccggacgg
    32761 gtcggagtta tcgcggttag ccatctggtt gccctgctct gtccagctac cggtgtagat
    32821 ccagcaagaa gatgcggttg taccgtcatc acgcagatgc gcaaagctac tcagcaactg
    32881 acctttcttc gcaatcagca cgccattagc atcatagaga tcttccagcg catagccgtt
    32941 gttctcttta gctacttcgt cagattgcgg ttcgtgcggc tgcttgtagt tccagctcat
    33001 cttcatcagc ggttctacgc ctttaccacc ttcagcctgg tacagctcgc gcagatgatg
    33061 gtagataccc gccagaattt cgccgtcgtt acgcgcttcg cccggcgcgt cctgaccttt
    33121 ccagtgccac tgcaaccagc gaccggagtt agcgatagaa ccatcttctt ctgcaaagca
    33181 ggtcgaaggc agacggaata cttcagtctg aatagacgcc ggatcgacat cgttcgactc
    33241 gccgtggttc tgccagaagg tagaggtttc agtcaccagc ggatcgataa ccaccatgta
    33301 cttcagcttg ctcaggcagc tcaccacttt gtttttgtcc gggaaggacg caaccgggtt
    33361 aaagccctgg cagaaataac cggtgacttt gccttcatcc atcatgttga aatacttgat
    33421 gacgtcgtag gtctggtccc acttcggcag ccagtcatag ccccagttgt tctctttctg
    33481 cgcggcatcg ccatagaaag atttcatcag gctaacgaag aacttcggat agttgctcca
    33541 gtagttcacc tgaccagcca gcgtcgcttt cggcgtgttc gcttccagat acgactgcaa
    33601 atcaacctgt ttttctgacg gcagcgtcag ataacctggc aggctggtag agagcagacc
    33661 taagtcagtc agaccctgaa tgttggagtg accacgcaat gcattcacgc cgccaccggc
    33721 catacccatg ttaccgagca gcaactggat catcgccata gtacggatgt tctgcgcacc
    33781 cacggtgtgc tgcgtccagc ccagcgcgta caggaaggtg gttgtgcgat ccggtgcgct
    33841 ggtggaggcc agcacttcac acactttcag gaagtcggct tttggcgtac cacagatgtt
    33901 ttcaacaacg tccggcgtgt agcgggaaac gtgctctttc agcaggttcc acacacagcg
    33961 cggatgagtc agtgtttcat cgcgttttgc atagccgttt tcatcgaact gatagttcca
    34021 ggacgactta tcgtactggc gtttttcagc gtcgtagccg ctgaacagac cgtcttcgaa
    34081 agcaaaatca tcacgcacca gcaggctggc gttggtgtaa tgcttaacgt attcggcgtt
    34141 gattttgttg ttttcgatca ggtagcgcaa aacgccagac aggaacgtaa tgtccgtacc
    34201 ggaacgaata ggcgcgtaga tatccgccac agaagcggta cgcgtaaaac ggggatcgac
    34261 aacgatcaag gttgcatcgt tgttgttttt cgcttccatc gcccagcgga aaccgacggg
    34321 atgcgcttca gcagcgttac cgcccattac cattacgacg ttagcgtttt tgatatccac
    34381 ccagtggttg gtcatcgcac cgcgaccaaa tgttggagca agacttgcta ccgttggtcc
    34441 gtgtcagacg cgcgcctggt tgtctaccgc cagcatcccg agggagcggg caaatttctg
    34501 ggtcagcatc ccggtttcgt tgctggcacc ggaagcacac agcataccgg tagaaagcca
    34561 acggtttacc gttacgccct gctcgttctt ttcaataaag ttagcgtcac ggtcagcttt
    34621 catcagcttc gcaatacggg agaatgcttc ttcccagcta atgcgctgcc atttgtcaga
    34681 acctggcgca cgatattccg ggtagcgcag acggttttcg ctgttgacgt aatccagcaa
    34741 accggcccct ttcgggcaca gcgccccacg gcttaccgga tgatccgggt caccttcaat
    34801 gtgataaatc gcttctctgg cgtttttcgc gccatcaccc aggctataca tcaatagccc
    34861 gcaacctacg gaacagtatg tgcaggtgtt acggatctct ttagcgcgta ataatttgta
    34921 gtttcgcgcc tgagccagtg cttgcttcgg ggcaaagccc aatgccgcta ctgttgttcc
    34981 agccataccg cccgcgcaga ttttaaaaaa ttgtctgcga ctgacgtcca ttgctttcct
    35041 cttttttcag gggtattact gcgcgaggaa actaacagta aaattctgaa tctttttgcg
    35101 cgaaatcaaa aagaacacgt gtttgccaca aaataatccg cctgcgcaaa ttgacctacc
    35161 tcaatagcgg tagaaaaacg caccactgcc tgacaggcca gttaaaaaaa tgctataaaa
    35221 ttcagcttaa tttttaacgg caagagagac aaaacagcga gcatgacacg acaaaaagca
    35281 acgctcatag ggctaatagc gatcgtcctg tggagcacga tggtaggatt gattcgcggt
    35341 gtcagtgagg ggctcggccc ggtcggtggc gcagctgcta tctattcatt aagcgggctg
    35401 ctgttaatct tcacggttgg atttccgcgt attcggcaaa tcccgaaagg ctatttactc
    35461 gccgggagtc tgttattcgt cagctatgaa atctgtctgg cgctttcctt agggtatgcg
    35521 gcgacccgtc atcaggcgat tgaagtgggt atggtgaact atctgtggcc cagcctgaca
    35581 attctctttg ccattctgtt taatggtcag aaaaccaact ggctgattgt accaggatta
    35641 ttattagccc tcgtcggcgt ctgctgggtg ttaggcggtg acaatgggtt acattatgat
    35701 gaaatcatca ataatatcac caccagccca ttgagttatt tcctggcgtt cattggtgcg
    35761 tttatctggg cagcctactg cacagtaacg aataaatacg cacgcggatt taacggaatt
    35821 accgtttttg tcctgctaac gggagcaagt ctgtgggttt actattttct cacgccacaa
    35881 ccagaaatgg tatttagcac gccagtcatg attaaactca tctctgcggc atttacctta
    35941 ggatttgctt atgctgcatg gaatgtcggt atactgcatg gcaacgtcac cataatggcg
    36001 gtaggttcgt attttacgcc cgtactttcc tcagcgcttg cagccgtgct gctcagcgcc
    36061 ccgctgtcgt tctcgttctg gcaaggcgcg ctgatggtct gcggcggttc cctgctctgc
    36121 tggctggcga cacgtcgtgg ctaaaataat tgccgggtct acggcccggc aattaatcag
    36181 ataaaccccg acaatattaa aaaataaatt tcaaatacca gtaattcacc ttatatctca
    36241 tttcgac
//
LOCUS       DAZKIP010000036        35485 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZKIP010000036 DAZKIP010000000
VERSION     DAZKIP010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.036"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            156..695
                     /gene="elfA"
                     /locus_tag="ACHOUK_002027"
     CDS             156..695
                     /gene="elfA"
                     /locus_tag="ACHOUK_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9897486.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGSVTFNGKVIAPACT
                     LVAATKDSVVTLPDVSATKLQTNGQVSGVQTDVPIELKDCDTTVTKNATFTFNGTADT
                     TQITAFANQASSDAATNVALQMYMNDGTTAIKPDTETGNILLQDGDQTLTFKVDYIAT
                     GKATSGNVNAVTNFHINYY"
     gene            778..1479
                     /gene="elfD"
                     /locus_tag="ACHOUK_002028"
     CDS             778..1479
                     /gene="elfD"
                     /locus_tag="ACHOUK_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP9897487.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSTWAAGKGGVGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKLVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            1504..4103
                     /gene="elfC"
                     /locus_tag="ACHOUK_002029"
                     /pseudo
     CDS             1504..4103
                     /gene="elfC"
                     /locus_tag="ACHOUK_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            4094..5164
                     /gene="elfG"
                     /locus_tag="ACHOUK_002030"
     CDS             4094..5164
                     /gene="elfG"
                     /locus_tag="ACHOUK_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9897488.1"
                     /translation="MQIIFGEKCVSLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTTGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            5192..5719
                     /gene="ycbU"
                     /locus_tag="ACHOUK_002031"
     CDS             5192..5719
                     /gene="ycbU"
                     /locus_tag="ACHOUK_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9897489.1"
                     /translation="MFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDVATKSA
                     LQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAPTLLKL
                     TDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKSTKAGAT
                     GGNATAVLYFDLVYQ"
     gene            5727..6242
                     /gene="ycbV"
                     /locus_tag="ACHOUK_002032"
     CDS             5727..6242
                     /gene="ycbV"
                     /locus_tag="ACHOUK_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9897490.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFSHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            6208..6944
                     /gene="ycbF"
                     /locus_tag="ACHOUK_002033"
                     /pseudo
     CDS             6208..6944
                     /gene="ycbF"
                     /locus_tag="ACHOUK_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            7055..8065
                     /gene="pyrD"
                     /locus_tag="ACHOUK_002034"
     CDS             7055..8065
                     /gene="pyrD"
                     /locus_tag="ACHOUK_002034"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFP9897491.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            8239..8781
                     /gene="zapC"
                     /locus_tag="ACHOUK_002035"
     CDS             8239..8781
                     /gene="zapC"
                     /locus_tag="ACHOUK_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFP9897492.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTNEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8778..9887)
                     /gene="ycbX"
                     /locus_tag="ACHOUK_002036"
     CDS             complement(8778..9887)
                     /gene="ycbX"
                     /locus_tag="ACHOUK_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFP9897493.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDSSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PEAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILTTAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            10131..12239
                     /gene="rlmKL"
                     /locus_tag="ACHOUK_002037"
     CDS             10131..12239
                     /gene="rlmKL"
                     /locus_tag="ACHOUK_002037"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFP9897494.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKEPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            12251..14158
                     /gene="uup"
                     /locus_tag="ACHOUK_002038"
     CDS             12251..14158
                     /gene="uup"
                     /locus_tag="ACHOUK_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9897495.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKNEEPAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            14288..15541
                     /gene="pqiA"
                     /locus_tag="ACHOUK_002039"
     CDS             14288..15541
                     /gene="pqiA"
                     /locus_tag="ACHOUK_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFP9897496.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCGTKGYVRRRNSLQWTLALLVTSIILYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            15546..17186
                     /gene="pqiB"
                     /locus_tag="ACHOUK_002040"
     CDS             15546..17186
                     /gene="pqiB"
                     /locus_tag="ACHOUK_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFP9897497.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     VLDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            17183..17746
                     /gene="pqiC"
                     /locus_tag="ACHOUK_002041"
     CDS             17183..17746
                     /gene="pqiC"
                     /locus_tag="ACHOUK_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFP9897498.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVAVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            18002..18169
                     /gene="rmf"
                     /locus_tag="ACHOUK_002042"
     CDS             18002..18169
                     /gene="rmf"
                     /locus_tag="ACHOUK_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFP9897499.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(18239..18757)
                     /gene="fabA"
                     /locus_tag="ACHOUK_002043"
     CDS             complement(18239..18757)
                     /gene="fabA"
                     /locus_tag="ACHOUK_002043"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFP9897500.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18826..20586)
                     /gene="ycbZ"
                     /locus_tag="ACHOUK_002044"
     CDS             complement(18826..20586)
                     /gene="ycbZ"
                     /locus_tag="ACHOUK_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9897501.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20772..21224
                     /gene="matP"
                     /locus_tag="ACHOUK_002045"
     CDS             20772..21224
                     /gene="matP"
                     /locus_tag="ACHOUK_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFP9897502.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(21300..22340)
                     /gene="ompA"
                     /locus_tag="ACHOUK_002046"
     CDS             complement(21300..22340)
                     /gene="ompA"
                     /locus_tag="ACHOUK_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFP9897503.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(22697..23206)
                     /gene="sulA"
                     /locus_tag="ACHOUK_002047"
     CDS             complement(22697..23206)
                     /gene="sulA"
                     /locus_tag="ACHOUK_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFP9897504.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            23425..24054
                     /gene="sxy"
                     /locus_tag="ACHOUK_002048"
     CDS             23425..24054
                     /gene="sxy"
                     /locus_tag="ACHOUK_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFP9897505.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKYPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(24017..26179)
                     /gene="yccS"
                     /locus_tag="ACHOUK_002049"
     CDS             complement(24017..26179)
                     /gene="yccS"
                     /locus_tag="ACHOUK_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFP9897506.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(26189..26635)
                     /gene="yccF"
                     /locus_tag="ACHOUK_002050"
     CDS             complement(26189..26635)
                     /gene="yccF"
                     /locus_tag="ACHOUK_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFP9897507.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIALIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26758..28812
                     /gene="helD"
                     /locus_tag="ACHOUK_002051"
     CDS             26758..28812
                     /gene="helD"
                     /locus_tag="ACHOUK_002051"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFP9897508.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVLIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28844..29302)
                     /gene="mgsA"
                     /locus_tag="ACHOUK_002052"
     CDS             complement(28844..29302)
                     /gene="mgsA"
                     /locus_tag="ACHOUK_002052"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFP9897509.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(29398..30060)
                     /gene="yccT"
                     /locus_tag="ACHOUK_002053"
     CDS             complement(29398..30060)
                     /gene="yccT"
                     /locus_tag="ACHOUK_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFP9897510.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            30233..30646
                     /gene="yccU"
                     /locus_tag="ACHOUK_002054"
     CDS             30233..30646
                     /gene="yccU"
                     /locus_tag="ACHOUK_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFP9897511.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(30691..31008)
                     /gene="hspQ"
                     /locus_tag="ACHOUK_002055"
     CDS             complement(30691..31008)
                     /gene="hspQ"
                     /locus_tag="ACHOUK_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFP9897512.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(31066..32256)
                     /gene="rlmI"
                     /locus_tag="ACHOUK_002056"
     CDS             complement(31066..32256)
                     /gene="rlmI"
                     /locus_tag="ACHOUK_002056"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFP9897513.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            32351..32629
                     /gene="yccX"
                     /locus_tag="ACHOUK_002057"
     CDS             32351..32629
                     /gene="yccX"
                     /locus_tag="ACHOUK_002057"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFP9897514.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(32626..32955)
                     /gene="tusE"
                     /locus_tag="ACHOUK_002058"
     CDS             complement(32626..32955)
                     /gene="tusE"
                     /locus_tag="ACHOUK_002058"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFP9897515.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(33046..33705)
                     /gene="yccA"
                     /locus_tag="ACHOUK_002059"
     CDS             complement(33046..33705)
                     /gene="yccA"
                     /locus_tag="ACHOUK_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFP9897516.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKMANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(34113..35132)
                     /locus_tag="ACHOUK_002060"
     CDS             complement(34113..35132)
                     /locus_tag="ACHOUK_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9897517.1"
                     /translation="MGRPRKNKKDNVLPPRVRSNGYSYVWKPEGSTRSIGLGRVRKTS
                     VAKVWQNYELEKAKLHNIMTVAKLWHMFMDSPAFTELAPRTQKDYRQHQKALLMVFGK
                     VLADNVKTEQVRIFMDKRGLESKTQANHELASLSRVYGWGYERGYVKNNPCKGVRKFS
                     LKARTVYITDEQYAAIYAEAIPQLRIAMEISYLCAARLGDVLELKWQDIMDKGIYIEQ
                     NKTGTKQIKEWSPRLRTAIQLARNVSSCTCEYVINTTKGGKVIAKTLNNWWNQAKRAA
                     EQKVGVPFGCNFHDIKAKGISDYEGSSRDKQIFSGHKTENQVLIYDRKTKITPTLDLP
                     LVVSK"
     gene            complement(35110..35352)
                     /locus_tag="ACHOUK_002061"
     CDS             complement(35110..35352)
                     /locus_tag="ACHOUK_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFP9897518.1"
                     /translation="MYELTLSPAEIKEITKYERYTKQQHQLRLHGIPFVIGPKNEPIV
                     LRRDIPHGLTTMPKTSELVSAEPDFEALNNGKTKKK"
BASE COUNT         9154 a         8422 c         9179 g         8730 t
ORIGIN      
        1 gtaatattta aatattcatg aaatctataa attaaagatt tgtcacttat tggatttagt
       61 atgcttgatt taaatggcgg acaattattg ttattattaa gtctaatgtc atttaaaggc
      121 attatcatat ttacgaagag atcaggatga taacgatgaa aaaaagtgta ttgacggcgt
      181 ttataactgt ggtatgtgca acgtccagcg ttatggctgc tgatgataat gctatcacgg
      241 atggctcagt aacatttaat ggtaaagtta ttgctccagc ttgtaccctg gtagctgcga
      301 cgaaagattc cgtggtgact ttgccagatg ttagtgccac gaagttgcaa accaatggtc
      361 aggtttctgg cgtgcaaact gatgtgccaa ttgaattaaa agattgtgat actaccgtaa
      421 caaaaaatgc aacgttcacc tttaatggca ctgcggatac tactcagatt acagcgtttg
      481 ctaaccaggc ctcatctgat gctgctacaa acgtggccct gcaaatgtat atgaatgatg
      541 gtacaacggc catcaagcca gacacagaaa ccgggaacat tttgttgcaa gatggagatc
      601 agacgttgac ttttaaagtt gattatatcg ctacggggaa agcgacttct ggtaatgtga
      661 atgcggtaac aaatttccat attaactatt attaatagaa ctcattaatt gttttattaa
      721 ttagtacccc tccagtgtta tggaggggat attcatattt tttaagagtg actatttatg
      781 aaaacttgca taacaaaggg aattgtgacc gtaagtttaa cggcaatatt actcagctgt
      841 tcgtccacat gggcggcagg taaaggcggg gttggacttg cagcaacacg tcttgtttat
      901 tcggaaggtg aagagcaaat ttcactgggt gtacgtaaca ccagtccgga tgttccttat
      961 ctgattcagt catgggtgat gaccccagat aataaaaaat cagcggactt tattattaca
     1021 ccaccgctat ttgtgctgaa tccggcaaac gagaatctgt tacgcattat gtacattggc
     1081 gcgccgttgg cgaaagacag agaaactctt ttcttcacta gcgtacgggc agtcccttca
     1141 acaacgaagc gggaagaagg caataccctg aagattgcca cacaaagcgt catcaaactt
     1201 ttctggcgac caaaaggttt agcgtatccc ttaggcgagg ctccggcgaa actgcgttgc
     1261 acttcgtcag ctgacatggt tacggtcagt aacccaacac cttatttcat taccctgaca
     1321 gacctgaaaa taggtggaaa gttagttaaa aatcaaatga tttccccctt tgataaatac
     1381 caattctctc tgccaaaggg tgccaaaaat agcagcgtaa cgtatcgaac catcaatgac
     1441 tacggggcgg aaacgccgca actcaactgt aaatcgtaat tcgtcttcag ttaagagagc
     1501 gagatgtata gaactcaccg acaacacagc ctgttaagct ctggtggagt gccatcgttt
     1561 attggtgggc tggtggtgtt tgtgtcggca gcgttcaatg cacaagctga aacctggttc
     1621 gatccagcct ttttcaaaga tgatccctca atggtggccg atttgtctcg tttcgaaaaa
     1681 ggacaaaaaa taacgccagg ggtttatcgt gtcgatattg ttctgaatca gacaattgta
     1741 gatacgcgca acgtcaattt tgttgagata acgccagaga aggggattgc cgcctgtttg
     1801 acgactgaaa gcctggatgc aatgggcgtg aatactgatg catttccggc ttttaaacaa
     1861 ctggacaaac aagcgtgtgc gccattggcg gagattattc cggatgccag cgtaactttt
     1921 aatgtgaata aactccgtct ggaaatttca gtaccgcaaa ttgccatcaa aagtaatgct
     1981 cgtggttatg tccccctgaa cgttgggatg aagggatcaa cgcgctatta ctgggatatt
     2041 catttagcgg ggctaacagt attcatagca gcgcagacag tgattctggc gacagttatt
     2101 ttctgaattt aaacagtggc gttaatttag gcccatggag attgcgcaac aattcaacat
     2161 ggagtcgtag tagtggccaa accgcagaat ggaagaatct cagcagctat ttgcaacggg
     2221 cggttattcc tctgaaaggc gaactgaccg taggtgatga ttatactgca ggcgattttt
     2281 tcgatagtgt cagctttcgt ggtgtgcagc tggcgtcaga tgacaacatg ctgccagaca
     2341 gcctgaaagg ggttgcgcct gtggtgcgtg gtatcgccaa aagcaatgcc cagataacga
     2401 ttaagcaaaa tggttacacc atttaccaaa cttatgtatc gcctggtgct tttgaaatta
     2461 gtgatatcta ttccacgtcg tcgagcggtg atttgttagt tgaaatcaaa gaagcggacg
     2521 gcagcgtcaa tagctacagc gtaccctttt ccagcgtgcc attactccag cgtcaggggc
     2581 gaatcaaata cgcggtgaca ctggcgaaat acagaaccaa tagtaatgaa cagcaggaga
     2641 gcaaatttgc ccaggccacg ttgcagtggg gcggaccgtg gggaacgacc tggtatggtg
     2701 gtggacaata tgctgaatat taccgtgccg ccatgtttgg tctgggattt aacctcggcg
     2761 atttcggagc aatttcgttc gatgtgaccc aggcgaagag tacgctggca gaccaaagcg
     2821 aacataaagg tcagtcatat cgttttctgt atgccaaaac gctcaaccaa ttgggtacta
     2881 actttcaatt gatgggctat cgctattcga cgtcgggttt ctacaccctt tccgacacca
     2941 tgtataaaca tatggatggc tacgaattta atgacggtga tgatgaagat acgccgatgt
     3001 ggtcgcgtta ttacaatttg ttttacacca aacgtggcaa actgcaggtc aatatctccc
     3061 agcaattaag cgagtacggt tcgttttatt taagtggtag ccagcaaact tactggcata
     3121 ccgatcaaca ggatcggcta ttacagtttg gctataacac gcaaattaaa gatctctcgc
     3181 tggggatctc ctggaactac agtaagtccc gtggccaacc tgatgctgat caggtgtttg
     3241 cacttaattt ttccctgcca ctcaatctgt tgctctccag aagtaatgat agctatacca
     3301 gcaaaaaaaa ttacgcctgg atgacctcta acaccagtat cgataacgaa gggcacacta
     3361 cacaaaacct gggtttaacg gagacactac tcgatgacgg taacctgagc tacagcgtgc
     3421 aacagggata taacagcgag gggaaaacgg ctaatggcag cgccagcatg gactacaaag
     3481 gggtgtttgc agatgcccga gtgggctaca actacagcga taacggcagt caacaacaac
     3541 tgaactacgc tctttcaggc agtttagttg cccattcaca gggcattacc ctggggcaat
     3601 cactggggga aactaacgtt ctgattgcag caccaggcgc agagaatact cgtgtggcga
     3661 acagcaccgg gctgaaaact gactggcgcg gatataccgt tgttccttat gccacttctt
     3721 atcgtgaaaa tcgaatcgca cttgatgcgg cgtcgttaaa acgtaacgtg gatcttgaaa
     3781 atgcagtagt caacgtggtt cccaccaaag gggcgttggt tctggcggag ttcaatgccc
     3841 atgcgggagc aagggtatta atgaaaacat caaagcaggg tatatcgctg cgttttggcg
     3901 cgatagcgac gctggacggc gtacagacta atagcggcat aattgatgat gatggctcgc
     3961 tctatatggc gggtttaccg gcgaagggaa caataaccgt gcgctggggc gaagctcccg
     4021 atcaaatttg tcatatcagt tacgagctta ccgaacaaca aattaactct gcgattacgc
     4081 gaatggatgc catatgcaga taatctttgg agaaaaatgc gtgtcattac tacgactatt
     4141 ttttgccgcc gtcttaatgc tatggtgcgc tcaaaccgct gcttatagcg ggcagtgtca
     4201 taccactcag gggaatccgt atattggcgt caattttggc gttaaaaccc tggaggaaga
     4261 agaaaatacg actggggtag taaaagacaa attttatcag tggaacgaat cgaatgatta
     4321 ttatgtttcc tgtgattgcg ataaagacaa tgtcagaagt ggccgatggg cattcgccgc
     4381 ggattcaccg ttagtctatt taggcgacaa ctggtacaaa attaatgact atcttgccgc
     4441 caaagtttta ttgcaggtta aaggcagttc tcctacagcg gttcctttcg aaaacgtggg
     4501 gactggggca gatacccggt ggcatatttg tgaccccggc ggtcaacgtt taggcggcca
     4561 gggagctagc ggtaatagcg gtagcttttc cctgaaaata ttgcagccgt tcgttggttc
     4621 ggtcgtcatt cctcctatgg cgctggcgcg attatttgaa tgctacaaca tacccgcagg
     4681 tgattcctgc acgactacag gcacaccggt tttagtgtat tacctgtctg gtactatcaa
     4741 ttcacttggc tcatgttccg tcaatgccgg agaaacaatc gaggtcgatc tgggcgacgt
     4801 atttgcggct aactttcgtg ttgtagggca taagcctctt ggggccagaa cggcagaact
     4861 tgcaattcca gtcaggtgta acacgggaaa cgcggggtta gttaacgtca acctgagtct
     4921 gacggcaacc acagacccca gctatcccca ggcgattaag acgtcacgtc ctggcgtggg
     4981 cgtggtggtg accgatagcc agaacaacat tatttcccct gctggtggaa cattaccgct
     5041 ctctattcct gatgatgcag acagtatcgc gcgaatgaat gtctatccag tcagcacgac
     5101 aggtgtacca ccagaaaccg ggcgatttga agccacggca acggtgagaa taaattttga
     5161 ttaacgcgtg aacgtatgaa gaaaaaaaac gatatttcag tgcgttatct tgttctttag
     5221 cattcttaac atccatgtcg ggatggctgg gcctgaacaa gttagtatgc atatttatgg
     5281 gaatgtggtc gatcagggct gtgatgtcgc caccaaaagt gcattacaaa atattcatat
     5341 tggtgatttt aatatcagtg attttcaggc cgcgaatacc gtaagcactg ctgctgattt
     5401 gaatatcgat atcaccggtt gtgccgctgg tattactggc gcggacgtcc tttttagcgg
     5461 cgaggctgac acccttgcgc cgacactgct caaactaact gacacaggcg gaagcggtgg
     5521 tatggcaacg gggattgccg tgcaaattct tgatgcgcaa agtcagcaag aaatcccgct
     5581 caatcaggtc cagcctctta cgcccttaaa agccggggat aacacactca aatatcaact
     5641 tcgttataag tccacaaagg cgggagcaac gggcggtaat gcgacggcgg ttctctattt
     5701 tgatctggtt taccagtgaa ggagtgatgt tgaaacggat aatctggatt ctgttcttac
     5761 tgggattaac gggggggtgt gagctatttg cccatgatgg cacggtcaac attagcggat
     5821 cgtttcgccg taatacatgc gtgctggcac aggatagcaa gcaaatcaat gtgcagttag
     5881 gcgatgtctc actgacacgt ttttctcatg ggaattatgg cccggagaaa tctttcatca
     5941 ttaatttgca ggattgcgga acagatgtca gcacggttga cgtcaccttt tcaggaaccc
     6001 ccgacggagt gcagagcgaa atgttgtcca ttgaaagtgg tacggatgca gccagcgggc
     6061 tggcaattgc gattctggat gatgcgaaga tattgattcc gcttaaccag gcgagtaaag
     6121 actacagcct acatagcggt aaggtgccat taacttttta tgcgcaattg cgacctgtca
     6181 atagcgatgt gcagtcgggt aaagtgaatg ccagcgcgac atttgtactt cattatgact
     6241 aatacgtgga acagattggc gctcttgatt ttcgccgttt tatcgttgct ggtggcgggt
     6301 gaattgcagg caggagtggt ggtcggcgga acgcgattta tctttccggc agacagagaa
     6361 tcgatatcta ttttactgac taataccagt caggaatcct ggcttattaa cagtaaaatc
     6421 aaccgcccaa cgcgttgggc cgggggtgaa gcgtcgacag tgccagcacc attactggcc
     6481 gctccgccac ttattctcct gaagcccggt acgacaggca cgttgcgctt gctgagaacg
     6541 gaaagcgaca tcttgcctgt ggatcgcgaa acgctatttg agttaagcat tgccagcgtg
     6601 ccatccggca aagttgaaaa tcagagcgta aaagtggcga tgcgctcggt atttaaactg
     6661 ttctggcgac ccgaaggttg ccgggcgacc cgctggaagc ttatcaacaa ttacgctgga
     6721 cacggaagtc gcagggtgta caactcacta acccaacgcc ttattacatt aacctgattc
     6781 aggtgagcgt aaatggtaaa gcgttaagta atgtgggagt tgtgccgcct aaaagccagc
     6841 gtcagacaag ctggtgtcag gcaatagcac cgtgtcatgt tgcctggcgg gcgataaatg
     6901 attacggcgg gttgagtgca aagaaggagc aaaatctgcc ctgaaacagg ttcggaaaac
     6961 gtttgcgttt tttttgccgc aggtcaattc ccttttggtc cgaactcgca cataatacgc
     7021 ccccggtttg cacaccggga atccaggaga gttcatgtac taccccttcg ttcgtaaagc
     7081 ccttttccag ctcgatccag agcgcgctca tgagtttact tttcagcaat tacgccgcat
     7141 taccggaaca ccgtttgaag cactggtgcg gcagaaagtg cctgcaaaac ctgttaactg
     7201 catgggcctg acgtttaaaa atccgcttgg tctggcagcc ggtcttgaca aagacgggga
     7261 gtgcattgat gcgttaggcg cgatgggatt tggatcgata gagatcggta ccgtcacgcc
     7321 acgtccacag ccaggtaatg acaagccgcg tctctttcgt ctggtagatg ccgaaggttt
     7381 gatcaaccgt atgggcttta ataatcttgg cgttgataac ctcgtagaga acgtaaaaaa
     7441 agcccattat gacggcgtcc tgggtattaa catcggcaaa aataaagata cgccagtaga
     7501 gcagggcaaa gatgactatc tgatttgtat ggaaaaaatc tatgcctatg cgggatatat
     7561 cgccatcaat atttcatcgc caaatacccc aggattacgc acactgcaat atggtgaagc
     7621 gctggatgat ctcttaactg cgattaaaaa taaacaaaat gatttgcaag cgatgcacca
     7681 taaatatgtg ccgatcgcag tgaagatcgc gccggatctt tctgaagaag aattgatcca
     7741 ggttgccgat agtttagttc gccataatat tgatggcgtt attgcaacca ataccacact
     7801 cgatcgttct ctggttcagg gaatgaaaaa ttgcgatcaa accggtggct taagtggtcg
     7861 tccgcttcag ttaaaaagca ccgaaattat tcgccgcttg tcactggaat taaacggtcg
     7921 cttaccgatc atcggtgttg gcggcatcga ctcggttatc gctgcgcgtg aaaagattgc
     7981 tgcgggtgcc tcactggtgc aaatttattc tggttttatt tttaaaggtc cgccgctgat
     8041 taaagaaatc gttacccata tctaattatt tcttcgaatt cctctttata agataatcag
     8101 ggctttattt tcagccctgg ttgttttata ttcattgctg ttgcttattt agacattttg
     8161 tacttttatt gttgaggtta ttaagcgaag cgacaatgga ttgtgttgct gcggttttat
     8221 agggtagggg agaggcagat gcgaattaaa ccagacgata actggcgttg gtattacgat
     8281 gaagagcacg atcgtatgat gctcgattta gccaatggta tgctatttcg ctcacgtttt
     8341 gcgcgcaaaa tgttgactcc agatgccttt tctcccgcag gcttttgcgt tgacgacgcc
     8401 gcgctctatt tctcttttga agaaaagtgc cgcgatttta atttatctaa agaacaaaaa
     8461 gccgaactgg tgctgaatgc actggtagca attcgctatc tcaaaccgca aatgccgaaa
     8521 agctggcatt ttgtttccca tggtgaaatg tgggtgccca tgccgggcga tgcggcctgc
     8581 gtttggttga gcgacactaa cgagcaagtg aatttactgg tcgttgaatc tggcgagaat
     8641 gccgcgctat gcctgctggc acaaccctgc gttgttattg cgggtcgcgc gatgcagttg
     8701 ggcgacgcca ttaaaatcat gaacgacagg ctgaaaccgc aggttaatgt tgacagcttc
     8761 agcctcgaac aggcagtcta acgcgccaac ttaagtgcag tcttcggtac acagctacag
     8821 caaagaatgg tgccatcatc gcccattgct gattttttca gcggcgtgac ttcgccttct
     8881 aaaagctgaa cacggcaact tccacaaatg cccgcgcggc aagaataagg gatacgaatt
     8941 ccctgatttt ctaattgctc cagcaacacc tgttggttat ttccacgaaa tgcctgtccc
     9001 tgccagtcaa tatctacatt ggcgtccggt tgttgcgtga tgttggcagt atcatcagcg
     9061 gcagctgcgc cgtaaatttt tgccggagcc gttgtcagaa tttccacctc atcgccaacg
     9121 cgaatcacgc cgctattacg ggcaattaaa ttctgaccaa aatcgacatc gccgttatcc
     9181 tgggcagtgc ggaaagattg caatgttttt aatggttcgc ctgccggatg tttttgccct
     9241 ttttctgggc tgacggtggt gaaaatacag cggctacaag gtttaaccac atcaaacacc
     9301 acatcaccaa tgcgaatcac tttccaacta tcttcttccc aggctgacgc gccggaaacc
     9361 accagattgg ggcggaattg ctccattttt acactggccg gacaacgttg ttggagatca
     9421 cgtaacgagg cttcgttagc aagaaggtaa ggatagccat cagcaaatga caggggtaca
     9481 gtgttgtggc gtttcacgcg ccgggtcatt tgtggcccca cccagcgtaa ttgcacttcg
     9541 cgggagaaaa atccacttaa ccatttgttg atcgcttctg gcgcaattcg cgcggtaaaa
     9601 tgtgtgcccc aaacttcggt tggtgcgtct tgtgtggcga aatcagcaaa acgaacatat
     9661 gcgctactgc tatctggtgc ggtgagatgc aagccatcat gcacgggtga aggagtaaat
     9721 cgtaccatct ggggaaactg gcgagcggtg ataaacgtac cgtcaggttc cgtgatcata
     9781 aagatgcgat cgaaggccag accactgaca tctgccagag catgtgtaag accaatgccg
     9841 cgcatcgatt taacaggatg aataaaaagc cgggttaatg tcgccacggt gcggtcctca
     9901 aatgaaaata agccctcaac tttatgacat gacgcgctta ttagctataa tgcgcaacaa
     9961 ttttcttagc gcctgtccca ttaggctatt ttatttgtca gtttggccct gggttgtgct
    10021 cgaaatcctc acgtacttgt gtacgctccg gtttctccgc gctgtccatg tccagactga
    10081 ctgcaacaat tacgcctact gcggtaggct cttagagtaa aagtgacgat atgaattctc
    10141 tgtttgccag tacggcccgt gggctggaag agctgttaaa aactgaactg gaaaacctgg
    10201 gggccgttga atgccaggtg gttcagggtg gggtccattt caagggcgac acacggcttg
    10261 tttaccagag cctgatgtgg agccgcctgg cctcgcgtat tatgttgccg ctgggcgagt
    10321 gtaaggttta cagcgattta gacctctatc tcggtgttca ggcgatcaac tggacagaga
    10381 tgtttaatcc tggcgcgacc ttcgctgtcc acttcagtgg tttgaatgac actatccgca
    10441 acagtcagta cggcgcgatg aaagtgaaag acgcgatcgt cgatgctttc acgcggaaaa
    10501 atctgccgcg tccaaatgtt gatcgcgatg cgccggatat ccgcgttaac gtctggctgc
    10561 ataaagaaac cgccagtatc gcccttgatc tcagtggtga tggtttacat ctgcgtggct
    10621 atcgcgatcg tgctggtatt gcgccgatca aagaaaccct ggcagccgct attgtgatgc
    10681 gatccggctg gcagccagga acaccgttgc tcgatccgat gtgtggttcc ggtacgttgc
    10741 tgattgaagc ggcgatgctg gcgaccgatc gcgcaccagg cttgcaccgt gggcgttggg
    10801 gctttagcgg ctgggcgcag catgatgaag ctatctggca ggaagtgaaa gcggaagcgc
    10861 aaactcgcgc ccgtaaaggc ctggctgagt atagctctca tttttacggt tcggacagcg
    10921 acgctcgggt gattcaacgt gcacgcacta acgcccgtct tgcggggatt ggtgaactga
    10981 tcacctttga ggtgaaagat gtcgcgcaac tgaccaatcc gctgccgaaa gagccgtacg
    11041 gcacagtgtt aagcaacccg ccatacgggg aacgtctgga cagcgaaccg gcgctgattg
    11101 cgctgcatag cctgctgggt cggatcatga aaaaccagtt tggtggctgg aatctctctt
    11161 tgtttagtgc ctcgccggat ctgctaagct gcttgcagct gcgtgcagac aaacagtaca
    11221 aggcgaaaaa cggcccgctg gactgcgtac agaaaaatta ccacgttgcc gaaagcactc
    11281 cagacagcaa accggcgatg gtagcggaag actacgccaa ccgcctgcgt aagaacctca
    11341 aaaaattcga gaagtgggct cgtcaggaag ggattgaatg ttaccgcctg tatgacgccg
    11401 atctgccgga atataacgtt gccgttgacc gttatgccga ctgggtggtg gtgcaggagt
    11461 atgcgccgcc aaaaactatt gatgcgcaca aagcgcgtca gcgtctgttc gatattatcg
    11521 ctgcaaccat ttcggtactg gggattgcgc caaacaaact ggtgctgaaa acccgtgaac
    11581 gtcagaaggg caaaaatcag taccagaaac tgggcgagaa gggcgagttt ctggaagtta
    11641 ccgaatataa cgctcacttg tgggtgaacc tgacggatta tctcgatact ggtctgttcc
    11701 tcgatcaccg tatcgcccgt cgtatgctcg gccagatgag caaaggcaaa gatttcctca
    11761 acctgttctc ttataccggc agcgccaccg tgcatgcggg attaggcggt gcacgcagca
    11821 ccaccaccgt ggatatgtcg cgtacttatc tggagtgggc agaacgcaac ctgcgtctga
    11881 atggcctaac cgggcgtgcg catcgcctga ttcaggccga ttgcctggcg tggctgcgtg
    11941 aggcaaatga acagttcgat ctgatcttta tcgatccgcc aaccttctct aactcaaaac
    12001 gaatggaaga tgcgtttgat gttcagcgcg atcatctggc gctgatgaaa gatttgaaac
    12061 gtctgctgcg tgcaggtggg acgatcatgt tctcgaacaa caaacgtggc ttccgtatgg
    12121 atctcgacgg cctggcaaaa ctgggactga aagcacaaga aattacgcaa aaaacgctct
    12181 cccaggattt cgcccgtaac cgccagatcc acaactgctg gctgattacc gcagcctgaa
    12241 aggaatagta atgtcattaa tcagtatgca tggcgcatgg ctgtcgttca gcgacgcgcc
    12301 gcttctcgat aacgcagaac tgcatatcga agataacgaa cgtgtttgtc tggtgggccg
    12361 caacggcgca ggcaaatcga cgttaatgaa aatcctcaac cgtgaacaag ggctggatga
    12421 cggtcgcatt atttacgagc aggatttgat tgtagcgcgt ctgcaacagg atccgccgcg
    12481 taacgttgag ggtagtgttt atgatttcgt tgccgaaggc attgaagaac aagcggaata
    12541 cctgaaacgc tatcacgata tttcgcgcct ggtgatgaac gacccgagcg agaaaaatct
    12601 caacgaactg gcgaaggttc aggaacagct ggatcaccac aacctgtggc agctggaaaa
    12661 ccgcatcaac gaagtgctgg cgcaactggg gttagatcct aacgttgcgc tgtcgtcgct
    12721 ttccggcggc tggttgcgta aagcggcatt aggacgcgcg ctggtgagca atccgcgcgt
    12781 attgctgctg gacgaaccga ctaaccacct ggatatcgaa accatcgact ggctggaagg
    12841 gttcctcaaa accttcaatg ggacgattat tttcatctcc cacgaccgtt cgtttatccg
    12901 caatatggcg acgcgcattg ttgatctcga tcgcggcaag ctggtgacct atccgggcaa
    12961 ttacgatcag tacctgctgg aaaaagaaga agccctgcgc gtggaagaac tacaaaatgc
    13021 cgagtttgat cgcaaactgg cgcaggaaga agtgtggatt cgccagggga tcaaagcacg
    13081 ccgtacccgt aatgaaggcc gcgtacgcgc cctgaaagcg atgcgtcgcg agcgtggcga
    13141 acgtcgcgaa gtgatgggta ccgcaaagat gcaggtggaa gaggccagcc gctccggtaa
    13201 gatcgttttc gaaatggaag acgtttgcta ccaggttgac ggaaagcaac tggtgaaaga
    13261 tttttctgcc caggttctac gtggcgacaa aattgccctg attgggccga atggctgcgg
    13321 caaaaccacg ctgctgaaac tgatgctcgg tcagcttcaa gcggacagcg ggcgtattca
    13381 cgttggcacc aaactggaag tggcttattt cgatcagcac cgcgcggaac tggatcccga
    13441 taaaacggtg atggataacc ttgccgaagg taagcaagag gtgatggtta acggcaagcc
    13501 acgccacgtt ttgggctatt tgcaggactt cctgttccat ccgaaacggg cgatgacgcc
    13561 agtacgtgcg ctttctggcg gtgagcggaa ccgcttgctg ctggcgcgtt tgttcctcaa
    13621 accaagcaac ttattgattc ttgacgaacc gaccaacgat cttgatgtcg aaacgctgga
    13681 actgctggaa gaactgatcg acagctacca gggcacggta ttgctggtaa gccacgatcg
    13741 tcagtttgtc gataacaccg ttacagaatg ctggatcttc gaaggcggcg gtaaaattgg
    13801 tcgttatgtc ggcggttatc atgatgcccg tggtcagcaa gagcagtatg tggcgctcaa
    13861 acagcctgcg gtgaaaaaaa acgaagaacc cgccgcgcca aaagcagaaa ctgtaaaacg
    13921 cagcagtagc aaactaagct ataaattgca gcgcgaactg gagcagctac cgcaattgct
    13981 cgaagatctg gaggcgaagc tggaagccct acagacgcag gtggcggatg cttccttctt
    14041 cagtcagccg catgagcaga cacaaaaagt gctcgccgat atggctgctg cagagcagga
    14101 gctggagcaa gcctttgaac gctgggagta tcttgaagcg ttaaaaaatg gtggctgatc
    14161 gcaaaatagt cgatttaaac agagacagct gacaacggta aaattgcctg atgaactggg
    14221 tttatcaggc atgaaaacaa acactttgtt agtcatctgt aaaatagcgc atcattaagg
    14281 agtaccaatg tgcgaacatc atcatgccgc gaagcacatc ctgtgctcgc agtgtgacat
    14341 gctggtggcg ttaccgcgcc ttgagcatgg tcagaaagcg gcatgtcccc ggtgtggcac
    14401 aacgttaacc gtggcgtggg atgccccccg gcagcgtccg accgcctatg cgttggctgc
    14461 actgttcatg ctgttgctgt ccaacttgtt tccttttgtg aatatgaacg ttgcaggagt
    14521 taccagtgaa attacattac tggaaattcc cggcgtgctt ttttctgagg actacgccag
    14581 cctcggcacc tttttcctgt tgtttgtgca actggttccc gcgttttgtc tgataaccat
    14641 tctgttactg gtgaatcgcg cggaattacc ggtccgttta aaagagcaac tggcacgggt
    14701 gctttttcaa ctcaaaacct ggggaatggc ggagattttc ctcgcgggtg tgctggtcag
    14761 tttcgttaaa ctgatggctt acggcagcat tggcgtaggc agcagctttc tcccctggtg
    14821 tttattttgt gtcctgcaac tgcgcgcttt tcagtgcgtt gatcgtcgct ggttatggga
    14881 cgatatcgcc ccgatgccag aactgcgcca gccgctaaaa ccaggcgtca cggggatacg
    14941 tcaggggctg cgttcgtgct cctgttgtac ggcaatcctt cctgctgatg aacccgtgtg
    15001 cccgcgctgt ggtactaaag ggtacgttcg acgtagaaac agcctgcaat ggacactcgc
    15061 gctgcttgtt acgtccatca tactgtatct tccggctaat attttgccca tcatggtgac
    15121 ggatttatta ggctcgaaga tgccatcgac gattctcgct ggggtcatcc tgttatggag
    15181 cgaaggctct tatcccgtcg ctgcggtgat ctttctggcc agtattatgg tgccaacgtt
    15241 aaagatgatc gccatcgcgt ggctgtgttg ggatgccaaa gggcatggca agcgcgacag
    15301 tgaaagaatg catttgattt atgaagttgt tgagtttgta ggccgctggt cgatgattga
    15361 cgttttcgtt atcgcggtgc tctcggcgct ggtgcgtatg ggaggtttaa tgagtattta
    15421 tccggcaatg ggtgcattaa tgtttgcttt agtcgtcata atgacaatgt tttctgctat
    15481 gacgtttgac ccgcgtttgt cgtgggatcg tcaacctgaa tcagagcatg aggagtcctg
    15541 acagtatgga atctaataat ggggaagcca aaatccagaa agtgaagaac tggtctcccg
    15601 tgtggatatt tcctatcgtc acggcgctca ttggggcctg ggttcttttt tatcattaca
    15661 gccatcaggg accggaagtg accctgatca ccgcgaatgc ggaaggaatt gaaggtggca
    15721 aaaccaccat taaaagccgt agcgttgacg tcggcgtggt tgaaagcgcc acactggctg
    15781 atgatttgac acacgttgaa atcaaagcgc ggctgaattc cggtatggaa aaattgctgc
    15841 ataaagatac cgtcttttgg gtggtgaaac cgcagattgg tcgagaaggg attagcggtc
    15901 tgggaaccct gctgtctggg gtttatatcg aactgcagcc aggcgcgaaa ggcagcaaaa
    15961 tggataaata cgatttgctg gactcgccac cgttggcccc gcctgatgcg aaaggtatcc
    16021 gtgtggttct cgatagcaaa aaagccgggc agctctcgcc aggagatccg gtgctgttcc
    16081 gtggctatcg ggtaggttcg gttgaaacca gcaccttcga tacgcaaaaa cgcaatatca
    16141 gttatcaact gttcatcaat gcaccttatg accgactggt gaccagcaat gttcgcttct
    16201 ggaaagatag tggcattgcg gttgatctga cgtcagcggg aatgcgtgtg gagatgggct
    16261 cattgacaac gctgctgagt ggcggtgtca gctttgatgt gccggaaggt ctggatttag
    16321 ggcagccagt ggcaccgaaa acagctttcg ttttgtatga tgatcagaag agcattcagg
    16381 attcgttgta caccgatcac attgattatc tgatgttctt taaagattcg gtacgcggtc
    16441 tgcaaccggg agctccggta gaattccggg gtattcgcct cggtaccgta agcaaagtgc
    16501 cattctttgc gccgaatatg cgtcagacat ttaacgatga ttaccgtatt ccggtgctga
    16561 ttcgtatcga gccagagcgg ctgaaaatgc agcttggcga aaatgcggat gttgttgagc
    16621 accttggcga attgttgaaa cgtggtttac gcggatcgct gaaaaccgga aacctggtca
    16681 ctggcgcact gtatgttgat ctcgatttct atccaaatac gcctgcaata accggtattc
    16741 gtgaatttaa tggttatcag attatcccga ccgttagcgg cggcctggcg caaatccagc
    16801 aacgactgat ggaagcgttg gataagatca acaaactgcc attgaatccg atgattgaac
    16861 aggcaaccag tacgctttct gaaagtcagc gcacaatgaa aaacctgcaa acgacgctgg
    16921 atagcatgaa caagatcctc gccagccagt cgatgcagca gttgccgacg gatatgcagt
    16981 caacgttgcg tgaattgaat cgcagcatgc agggcttcca gcccggctcc gcagcctaca
    17041 acaagatggt ggcggatatg cagcgccttg atcaggtgtt gcgagaactg caaccggtgc
    17101 tgaaaacgct caatgagaag agtaacgcgc tggtatttga agcgaaggac aaaaaagatc
    17161 cagagccgaa gagggcgaaa caatgaaaaa gtggctagtg acgattgcag cactgtggct
    17221 ggccggatgc agctccggcg aaattaataa aaactattac cagttacctg tggtgcagag
    17281 cggtacacaa agtaccgcca gccagggcaa tcgcctgtta tgggtagagc aggtcgctgt
    17341 acctgactat ctggcgggga atggtgtggt ttatcaaacc agtgatgtga agtatgtgat
    17401 tgccaacaac aacttgtggg ccagcccgtt ggatcaacag ttgcgcaaca ccctggtagc
    17461 caacctgagc acgcaactgc ccggctgggt ggttgcctcc cagcctctgg gaagcgccca
    17521 ggacacgctc aatgttaccg taacggagtt taacggtcgc tatgatggca aggtcattgt
    17581 cagtggtgag tggctgttga accaccaggg acaactgatc aaacgtccgt tccgtctgga
    17641 aggagtgcaa actcaggatg gttacgatga gatggttaaa gtgctggccg gtgtctggag
    17701 tcaggaagcc gcttctattg cacaagagat aaagcgtcta ccttaattat aaagatttgt
    17761 aaatataacc gtctccggta tgttgcctga ggcggttttt ttgtttctaa cgtgcggaaa
    17821 aatttgttcc tcttcacatt ttttgtacaa ccgacatgcc cgtgtagctc acaaatatga
    17881 cagtggcgtg aattttgcgc attgacggca gttatgattc gcggtattgc ttaactgtga
    17941 ttgcacattt agtaatcact gttttctttt ccaccagaaa ccagtatgag ggaaacgagg
    18001 catgaagaga caaaaacgag atcgcctgga acgggcacat caacgtggtt atcaggccgg
    18061 catcgccgga cgctcaaaag aaatgtgtcc ctatcagacg ctgaatcaaa ggtcacaatg
    18121 gctgggaggc tggcgagaag ccatggcgga cagggtagta atggcctgat tctgtctctt
    18181 taaaaagaaa cctccgcatt gcggaggttt cgccttttga tactctgtct gattataatc
    18241 agaaggcaga cgtatcctgg aacagaccga ctttcaggtc gctggcggta tagatcagac
    18301 gaccatcaac cagcacttcg ccatccgcca ggcccataat cagacgacgg ttaacaatgc
    18361 gtttaaagtg aatacggtag gtcacttttt tcgctgtcgg cagtacctga ccagtgaatt
    18421 tcacttcgcc aacgcccagc gcgcggcctt taccttcgcc gcccagccag ccgaggtaga
    18481 accctaccag ctgccacatt gcgtccaggc ccaggcatcc cggcataacc ggatcgccaa
    18541 taaagtggca tccgaagaac cacagatccg gattgatatc cagttctgct tcaacatacc
    18601 ctttgtcgaa gttaccaccc gtttcggtca ttttgaccac acggtccatc atcagcatgt
    18661 tcggtgctgg caattgcggg cctttagcgc caaacagttc accgcgacca gaggcaagaa
    18721 ggtcttcttt tgtataggat tcgcgtttat ctaccatgtt ctctgtaagc cttattttat
    18781 tgaagcacgc aggatagcta acacgtgtac gctgaacaag tccgatcagt tcggaataaa
    18841 ccagttcagc caacgtaatg gccatggaaa acggtgacgt ccttcctgtt gcgatgcttg
    18901 cgcgatacgt tcctggatgg tttgcatcag cgtcgtttgg ccttcgccat cccacaccag
    18961 atttaataat aacggcagtg cgtcagtcac atcgtctact gcccagatgg tgaatttgcc
    19021 ttcttctacc gctttcacca gttcactgtg aagacttaaa tggcgaacgt tagctgtggg
    19081 gataatgaca ccttgtttcc cggttaactc acgttgctgg caaatagcaa agaagccttc
    19141 gattttctca tttaaaccac cgaccggctg ggcgcgaccg aactgatcga ctgaacctgt
    19201 gatagcgata ctctgattca ccggcacatc ggcgagggcg cttatcaggg cgcagagttc
    19261 agccatcgag gcactatctc catcaacttc actgtatgac tgctcaaatg tcagcgatgc
    19321 tgagaagggg atctgttgct caagctgtag ttccgacatc aggaacgctt gcatgatcat
    19381 catccctttc gcatggatat tgccgccaag ctccgctttg cgttcgatgt cggtgaattc
    19441 accatcgcca atatgcacaa cgcagctaat gcgagaaggt tcgccaaaag cgcgtggatg
    19501 acccggaaat tcaatgaccg aaagggcgtt aatttgcccg atgcgttcgc cttcggtttc
    19561 aatcaggatt tgctcctgaa ggatctcatc ctgcatacgt tcagcgagga aaccttcgcg
    19621 ccattcacgc tgctgcagca ttaagtttag ctgctcgccg gagaaggtgt cgccatcaca
    19681 cagggaggcg acctctttac actggcggag gatccactgc gggctaagcg gaagtgtttc
    19741 ttgttcaccg gtgtagcgtg ctgcttcgcg gataagtacc ggccaggcat ccgctcccgg
    19801 ggcaggtaag tgattatgtc tggcggtaaa tgtcacccag cgacaccact gggttactga
    19861 ctccgcatcg acaatctgca gagtatcttc aaattcgcta taaatagcct gctctgaaag
    19921 ctctggctcc atctcctgga aatcagccaa tgattcgcgt tcgcctacca gaatgacttt
    19981 cagcttcaat ggcatcgaag gcacagagac ggggagaggg cgcgactcat caaacgcaac
    20041 ccagtcaaaa cgctcgcggt taacgatatt tttcagccgc atccacagca gaggttgcgc
    20101 cagcagtgta cgcaaagaga tgatgagaat accgccattt gcctgatgca ccagaccagg
    20161 ctgcagggta atgtcgccat taaactggcg caggcagcca aagagttgct ccgcttctac
    20221 ccagtcggca gcgacaactt gcgttaaagt cgcaaaatta tcatctgcac tcactgcgtg
    20281 acgtaagcgg atggagtggc cggaaacctc atagtgaccg cccaccagtt ggcctgcatc
    20341 gctttgtagc gtacgcgcgg cattggcaat aagattcaga tactcagact cttccggggc
    20401 cttcgccagc ataaaagagg aggatgctcg cgtatgcagc aactgctcca gcgcaaattg
    20461 cagccgcggt tgagtatcac tgaataaagg atcgttttcg tcaatcaaat gtggctgagc
    20521 aaatatttcc tgatagctat cggtatcagg aaccaggtca cgccatgcaa gtttcgtaat
    20581 ggtcaaagtt gatgtttttt agtctgttgt caaagccgcg attataccgt aaccggcact
    20641 acagcacacg tagaaagcac cgacaatact cctggcatgg gcgttaaagc tcacaggatg
    20701 gagattcttt tcttcactgg cctaaaaagc tgatattctg taaagagtta cacggtaaca
    20761 ttgagatcgc tatgaaatat caacaacttg aaaatcttga aagcggttgg aaatggaagt
    20821 atctggtgaa gaagcatcgc gaaggggagt taatcacccg ttacatagaa gccagtgccg
    20881 cccaggaagc cgtcgatgtg ttgctctcgc tggaaaatga acccgtgctg gtaaatggct
    20941 ggattgacaa gcatatgaat ccggaactgg tcaatcgcat gaagcaaacc attcgggcaa
    21001 gacgaaagcg ccattttaat gcggaacacc agcatacgcg caaaaaatcg atcgatctgg
    21061 aatttattgt ctggcaacgt ctggctggtc ttgcgcagcg tcgcggtaaa acgctttctg
    21121 aaacgattgt tcagctgatt gaagatgcgg aaaacaaaga gaaatacgcg aataaaatgt
    21181 cttctctgaa gcaggatctg caggcattgc tgggtaagga ataactgacg aaagtcagtt
    21241 caatttacta aaggcaaaaa aaaccccgca gcagcggggt ttttctacca gacgagaact
    21301 taagcttgcg gctgagttac aacgtctttg atacctttaa cttcgatctc tacgcgacga
    21361 tccggagcca ggcagtcgat cagtgcagca cgctgtttca cgttgtcaca ggtgttgcca
    21421 gtaaccgggt tggattcgcc cataccacgt gcggagatct tgtctgccgg gatacctttg
    21481 gagatcaggt aatcaacaac agactgagca cggcgctcgg acagaccctg gttgtaagcg
    21541 tcagaaccga tgcggtcggt gtaacccaga acaactacgg aaccgtcttt cggatccagg
    21601 ttgctcagct ggctgtacag ctgatccaga gcagcctgac cttccggttt cagggttgct
    21661 ttgttgaagt tgaacagaac gtcagacttc agagtgaagt gcttggtctg tacttccggt
    21721 gccggagctg gagccggagc aactactgga gctgcttcgc cctgaccgaa acggtaggaa
    21781 acacccaggc tcagcatgcc gttgtccgga cgagtgccga tggtgtgtgc gtcaccgatg
    21841 ttgttggtcc actggtattc cagacgggta gcgatttcag gagtgatcgc gtactcaaca
    21901 ccgccagcga agaccggaga aacgccggtg tcgtggtttt taccataaac gttggattta
    21961 gtgtctgcac gccataccat accacccaga cgagtgtaga tgtccaggtc gtcagtgatt
    22021 gggtaaccca gtttagcggt cagttgaacg ccctgagctt tgtatgcacc gttttcaacg
    22081 ctgcctttgt acggcatacg acctaaccag tcgtaaccca tttcaaagcc aacatacggg
    22141 ttaacctggt aaccaccaaa agcaccagcg cccagttggt tttcatgggt cgggccattg
    22201 ttgttgatga aaccagtgtc atggtactgg gaccagccca gtttagcacc agtgtaccag
    22261 gtgttatctt tcggagcggc ctgcgctacg gtagcgaaac cagccagtgc cactgcaatc
    22321 gcgatagctg tctttttcat tttttgcgcc tcgttatcat ccaaaatacg ccatgaatat
    22381 ctccaacgag ataacacggt taaatccttc accgggggat ctgctcaata ttaactctac
    22441 cgatatcttc ggcttatgcc gagcacccct ggcgatgtaa agtctacaac gtagttgaaa
    22501 acttacaagt gtgaactccg tcaggcatat gaaaaaaaag tcttgtataa ggtatgttta
    22561 atcttttttg tcagcgacaa tttacagaag agaatcgcgg aaaccgcttc agacaagcct
    22621 ccgcaaggaa aattagtcac gactgaaagc attggctggg cgacaaaaaa agttccagga
    22681 ttaatcctaa atttacttaa tgatacaaat tagagtgaat ttttagcccg gaaagttgtc
    22741 tcgtggcgtg agaggatgcg cttaccggac gcataataaa ccccatagcg ttaccttcat
    22801 ttgccgcatc aacaagttca gcatgctctt cttcagtcaa atcatctgcc aaccaaccga
    22861 tcaccacact gtaattgccc gtgcgtaaag cgcgaaccat tgactccaca gtgtggcaag
    22921 gggagagctg gctaatctgc attactttcg ttaagggtag cccagatgcc tgaacccatt
    22981 cccgactcag tttttgttgc ggtgttaacc agagttgcca gcgcgattgc tgaccgagtt
    23041 gctgtaacaa tggcaacagt agaagttgcg tcatcatggg ctgatcttcg cgatagacaa
    23101 cttcactgat aagcccggct gtagtgtttt ccgtagagac acgcgcaatt ttacttgctg
    23161 cggatgagaa cgacgaagaa cgatgtgcat agcctgaagt gtacataatc aatccagccc
    23221 ctgtgagtta ctgtatggat gtacagtaca tccagtgaca acaaagatca accctatttt
    23281 cggaaagagc ctcgcaaatt ttgtcgttgg tgacgggaaa acataaatta atcttgccgc
    23341 ttaagaataa gttgcctatt ttcgtagtta acggatccgt taatgtgaat cattctttta
    23401 tgttatgatt ttaaaaggaa ttttatgaaa agcctctcct ataagcggat ctataaatca
    23461 caagaatacc tggcaacgtt gggcacaatt gaataccgat cattgtttgg cagttacagc
    23521 ctgaccgttg acgacacggt gtttgcgatg gtttctgatg gtgagttgta tcttcgggct
    23581 tgtgagcaaa gtgcacagta ctgtgtaaaa tatccgcctg tctggctgac atataaaaag
    23641 tgtggccgat ccgttaccct caattactat cgggttgatg aaagtctatg gcgaaatcaa
    23701 ctgaagctgg tgcgtctgtc gaaatattct ctcgatgcag cgctgaaaga gaaaagcacg
    23761 cgcaataccc gggaaagact gaaagatttg cccaatatgt cttttcatct ggaagcgatt
    23821 ctcggggagg tggggattaa ggatgtacgg gcgttacgta tacttggggc aaaaatgtgt
    23881 tggttgcgac tgcggcagca aaacagtctg gtgacagaaa agattctgtt tatgcttgaa
    23941 ggtgccatta tcggcattca tgaagctgcg ctcccggtgg cacgccgcca ggagcttgca
    24001 gaatgggctg actctcttac gccgaaacag gagtttcctg cggaacttga gtaatctggc
    24061 gttgcagacg accaatctca ggcagtaatg caatcaataa tccaacttgt tgtacgacca
    24121 gaggttcttt gctgtctgcc cgtggttcaa gttgctgcat ccgctgtttc aggctagcta
    24181 atgcctcatt gacgcgttct tcatcagcag gttgatgatg taacgcgtca tcaacatagc
    24241 aaactgcgtc atcaagaaac gccagaattt caggattagt taactgctcc cggtgagcac
    24301 cgagggctga gatatagctg gtaaacgtat ggttaaggca cagcaaccga aacgcggctt
    24361 cgcgaatttg cggggtaacg ttcggctcgc tggacatatt tgataccacc gacgccagct
    24421 cagcatcacg gttgtgtgca tcgcggcggg caatacgata cgccagacgg ttatcacgcc
    24481 cctgatggta ttgctccagt atggcatcga gataccgaca gttggcctct gtggcgcgtt
    24541 cgagcatgcg cggcagattg cgaaactgcc agtcaggcca gatgtagctc actgccgccc
    24601 acgcaatggc acaaccaatc agcgtatcga ttacgcgagg taacgctact tcaaaacctt
    24661 cacccagtaa gttaaaacac agtagcacca aaagtgtgat gaacatcgtt gcatgagcgt
    24721 attgcacgtt acggaaggca aaaaagagca cgccggtaat aaccagcagc accagctgcc
    24781 cttccagtga tggcacaaac cacagcacag gaatgccaat ggcgatacct accagcgtac
    24841 caataatcct taacttcagg cggtggcgcg tggcgttata gtttggctgg cagacaaaca
    24901 aacttgtcag caagatccaa tacccgtgat gcattccggt tatctgaatg atggcgtagc
    24961 cgaagcacaa caccagcgac attcttaccg catgacggaa gagggcggat tccggcgtga
    25021 agtgacggct aagacgcagc cagatatcac tcaacccgtg cgggctgtca tcagcgagct
    25081 cattttcgtc attattatgg ggtagtgcct gggcctgttc tgattcaatt gttgccagtt
    25141 gggcatcaat ggcgcgtaaa ttgttcagca aaaatcccag tgttttgagt aaatcggcgg
    25201 gtgcgccgtt atcgcgcatc cgctccagcg cagcatcaat atgcgtaaaa gcgcgctcaa
    25261 aatgcggatc atgttgataa ggctgacgca acaaaataca gcgtgacagt tgctggcacg
    25321 cctggccctg catcgacatc agccgctgaa aacggaacag cacgtcgctg tggcgaaaat
    25381 gttcacgcaa tgtttgatac tgaatatgag aagagctggc acgctcgtga atatcctgtg
    25441 cgacaaagta ataatgcagc gtgcgacgcg ttccccgttg accacgatcg ccacgtaagc
    25501 gggtcagcag cgagagtttc gtctgattca atgtcgccat cagcagaccg ttggcgagag
    25561 ccaaatcgta cagcggtgcc tggctttcat cttcaatatc aggatcaaac atgcgcgact
    25621 tgagctcaag ataacgcgcc agttgttcat agcaacgcgc caggttgtcc tgcagcgggc
    25681 ggaccgggaa cagcagatga ccaataagtg tcaggacgtt gtaccagacg gcaccggcca
    25741 gcagatacat cggctgctga taccagtgct catacagtga tgttcccaac atagtgtaaa
    25801 tggcgatcag caatgcaccg aaggcaattg ttgcatagcg ttgacccaga ccgccgagca
    25861 aaatgaagcc gctggtagag agcgttaagc caatcgcaaa tagccagggc cagggaaaca
    25921 gcaattctac tgaggccgag gcgataaaaa agcagaacag cgtaatgatg aggttacgca
    25981 aacgtcccgc cagtcggtca tcgagatcgg tcagcgccgc tgccaccatc cccagcgtta
    26041 gcggaatcgt cagttttaca tcacccagcc accacggaaa cgctgtggtt ccacaaagcg
    26101 caataaaaat acgcgcgtaa tacagccagg cgctgttcca ggtatagcgt ttgagcaaag
    26161 gacttagcat aaaggccata ttagtggatt attcaaaacg acgacgtgca ttggcttcac
    26221 gcgcagcttg cgctgtttct accgatacca cgcgacgacc aaccggccat agtgcaatag
    26281 cggcaatttt aaagttcgca atgccgacag gaatgccaat gattgaaata cattgtgcga
    26341 tgcccgttgc aatgtgcatc aggcataacc accagccaaa gaaaatcagc cagaaaatat
    26401 tcaataccgt accgccagta ttcagcagca catttttgcc agccgggttc agttcatcga
    26461 catgaatagc ttcattgcca taaggcacca gagacagttt agtgatctcc cagcaggatc
    26521 gtgtcagcgg taaggtaaaa atcagcgcaa tactgaccag agtcgccaac agccagccca
    26581 gagtggtggc aaatccgcca agcacaaagt tcagaatgtt caaaacggta cgcataaaac
    26641 ctcgctttac tgtggttttc agtaatgggc ggcaattgta acgttttttt gggctggagc
    26701 acgttttctc tgacggttac actgataaga aataatttcg tgtggatcta cagagtcatg
    26761 gaactgaaag cgacaacgct tggaaaacgt ctggcacagc acccttacga tcgggcggtg
    26821 atcctcaatg ccgggattaa agtctccggc gatcgccacg aataccttat tcctttcaat
    26881 caattactgg cgattcactg taagcgcggt ctggtatggg gcgagctgga atttgtactg
    26941 ccggacgaaa aagtggtgcg tctgcacggt accgaatggg gcgagacgca gcgtttttac
    27001 catcatcttg atgctcactg gcggcggtgg agtggcgaga tgagcgaaat tgcgtctggt
    27061 gttttacgcc agcaactgga tttgattgcc acgcgcactg gagaaaataa atggctgacg
    27121 cgtgagcaaa cctctggcgt tcagcaacaa atccgccagg ctttgtcggc gttgccgttg
    27181 ccggttaacc gactggaaga attcgataac tgccgtgagg cgtggcgtaa atgtcaggcc
    27241 tggttgaaag atattgaaag cgctcggttg cagcataacc aggcgtatac cgaagccatg
    27301 cttaccgagt atgcggattt tttccgccag gtcgagtctt caccgctgaa tccggcgcag
    27361 gcccgggcag tcgttaatgg cgagcattct ctgttagtgc tggcaggtgc aggaagcgga
    27421 aaaacgtcgg tgctggtggc ccgtgcaggc tggttgctgg cgcgtggtga agcgtcccct
    27481 gagcaaattt tattgctggc gtttggtcgc aaagccgctg aagagatgga cgagcgtatt
    27541 cgcgaacggc tacataccga agacattacc gcacgcacgt ttcatgcgct ggcgctgcat
    27601 attattcagc agggcagcaa aaaagttccg atagtcagca aactggaaaa tgataccgct
    27661 gcccgtcatg aactctttat tgctgagtgg cgcaagcaat gcagcgaaaa gaaagcgcag
    27721 gcgaagggct ggcggcaatg gctgacggaa gaaatgcagt ggtcagtgcc agaaggtaac
    27781 ttctgggatg atgaaaaatt acagcgtcgt cttgcctcac gcctcgatcg ttgggtaagt
    27841 ctgatgcgga tgcacggtgg tgcacaggca gaaatgattg ccagtgcacc cgaagagatt
    27901 cgcgatctgt tcagtaaacg tatcaagttg atggccccgt tattaaaagc ctggaaaggt
    27961 gcgctgaagg cagaaaacgc tgtcgatttt tcgggcctta ttcatcaggc gattgtgatt
    28021 ctggagaaag gtcgctttat cagcccgtgg aagcatattc tggttgatga atttcaggat
    28081 atctcgccgc agcgggcagc gttgttagcg gcattacgca agcaaaacag tcagacgacg
    28141 ttgttcgccg ttggtgatga ctggcaggcg atttaccgat tcagcggtgc gcaaatgtcg
    28201 ctcaccaccg ctttccatga aaactttggt gaaggcgaac gctgtgattt agacacgact
    28261 taccgtttta acagtcgtat cggtgaggtg gcaaaccggt ttattcagca gaacccaggc
    28321 cagctgaaaa agccgctaaa cagcttaacc aatggagaca aaaaagccgt cacgttattg
    28381 gatgagagtc aacttgacgc tttgctggat aagctctctg gttatgccaa accggaagag
    28441 cgcattctga tcctggcgcg ttaccatcac atgaggcctg ccagcctgga aaaagcggca
    28501 acacgctggc cgaagttgca aatcgacttt atgaccattc atgccagcaa agggcaacag
    28561 gcggattacg tcctcatcgt tggcttgcag gagggaagtg atggttttcc ggctgcggcg
    28621 cgggagtcga ttatggaaga ggcgctactg ccaccggttg aggatttccc ggacgctgaa
    28681 gaacggcggt taatgtacgt ggcgctgacc cgggcacgtc atcgggtatg ggcactgttt
    28741 aacaaagaga atccctctcc ctttgtggaa atactgaaaa atctggatgt gccggtggcg
    28801 agaaaaccgt aagaaacagg tggcgtttgc cacctgtgca atattacttc agacggtccg
    28861 cgagataacg ctgataatcg gggatcagaa tatcgaccgc gtcgttgaaa tgcggcgact
    28921 ggataatgaa gtctgccgtt gccacgttgg tggcgaccgg aatgttccat accgtcgcca
    28981 gacgcagcaa ggctttcacg tcaggatcgt gcggcacggc atttagtgga tcccagaaga
    29041 aaatcaatac atcaattttc ccttctgaga tcaatgcgcc aacctgctgg tcacccccca
    29101 ttgggccact caacatcgcg ttgacgttca tgccggtcgc gcgggaaatt aagttaccgg
    29161 tagtgcctgt tgcatacagt acgtgttgtt ccagtaacgg ttgatgccgt tccacccagc
    29221 tcatcagcat ttgtttgcag tgatcgtgtg ccaccagcgc aatatgtttc cgcgcaggta
    29281 aagtgcgagt cgtcagttcc ataatgtaca tccgtagtta actttcctac agattactgt
    29341 aagcacttat cgctgcaaga taaagaccga aaaagcctgc gcacaggcac aaaaatctca
    29401 ggaagatggt tgtttttccg cccattgcag gaaagtattt cgcgtttgtg ggtcagccag
    29461 tttaaaccaa tacttcagcc gttgttctgt gagcacctga gactgcggtg gaatagcatc
    29521 cagctcggaa acacccgaaa gcagcgtact gtcatctgcc atcatcgtgg caaattgcgg
    29581 tagtgaagcg cgtttcgcgg atttgttata gcgttcaacc tcgacctcat aatcaatcgt
    29641 ttttgccgtt gaggtaatgg cgagaatatc cagctttacc ggaatcggcg tcgcatcgcc
    29701 atccaacaat tcaaggcgcg gcgcggcatc aaaatggtta gcttcccgct cattctccag
    29761 gcgaggcagg cgaaaattga cctggttgat gagctgggta ttaaaactga ccaccagcgg
    29821 tggggagata tacagccgtt cttcactatt ggaaagatga atcgtcttct caacgcgaaa
    29881 cactaactga tgcggtccat tatccagttc aatgctgtcc gcaccgcgca gcagagagct
    29941 ggaaaccttc ttgccgtcga gtaccagaag atcgacatcg gttgaaagcc gcagcgtggt
    30001 ggcaaaaacg gataccggca aacataatgc aatcaaggtg gtcacgatgc cggttttcat
    30061 aggtagctcc tgtcaaaaga ccgcccgaat aataatgtgt caaaattttt ctttaatcat
    30121 acttactaac atatagacat atttcccgct tttgctctca ttcattcgta ttagctgcat
    30181 ggttggcatg tcggctttgt cgtacacttt gtgaaacagt ctggaggaaa taatgaaaga
    30241 aaccgatatt gcaggcattt taacgtccac tcacaccatt gcgctggtgg gggcaagcga
    30301 caaacccgat cgtcccagct atcgcgtgat gaaatatcta ctcgaccagg ggtatcacgt
    30361 cattccggtt tcgccaaaag ttgccggcaa aacgctgctg ggccagcagg ggtacggcac
    30421 gctggcagac gttccggaaa aagtcgatat ggtcgatgtt ttccgtaatt cagaagcggc
    30481 gtggggcgta gcgcaggaag ctatcgccat tggcgcgaaa accctctgga tgcaactggg
    30541 ggtgattaat gagcaagcgg cagtactggc acgggatgcc gggttgaatg tggtgatgga
    30601 tcgctgcccg gctattgaga tccctcgcct gggcctggcc aaataaaaaa tccccggaag
    30661 gcaaaaacct tccggggatc tgttcaggga ttagttacgc agacgcgggg cctggagttg
    30721 tttgcggatg gtctgcgcca gctcatccat ggatggctgt tcaggatgtt catcctgcaa
    30781 ttcgctactt agctgggctt cagccaggta ggtatgaacc ggtaggccgt tatcgtcctc
    30841 catcaccacg tgataccacg gagcggcgcg aagctcgtca ttcaccgcca gctcatcagg
    30901 cgacggttca gagagcgaat aaaccgggtc gatatccacg accactccga gataacctaa
    30961 cagggaatgg cggacctgct ggccgatacc gaatttgctg gcaatcatag tcacctcccg
    31021 ggaaatcttt acctttgata taagggtaaa attccacatt tcaagttaca tgacgcgaca
    31081 ggcaaaccct ttcagatata gcccttccgg ataggtagcg atcaccggat gatcggccgc
    31141 ctgacggaac tgctctataa attgtacatc acgaccggca tcaattgcgg catccgcgat
    31201 gattttctga aataaatcgc tggtcatcag accggaacag gagaaagtca ggagaatacc
    31261 gccttcattc agcagctgaa tcgccagcat gttgatatct ttatagccac gacacgcgcc
    31321 catcaactgg cttttattct caacaaactt cggcgggtcc atcacgataa cgtcaaattt
    31381 ttcaccgcga tcgcgatagg tacgcagcaa tttaaagaca tcatcacgga caaactcagc
    31441 cttgctcaga tccagtttgt tcagctcaac gttctgccgt gcaatatcca gtgcttcctg
    31501 ggaggtatca acgctgacaa cctggctgca accgcccatc agtgccgata cggcgaaacc
    31561 accggtatag gagaaacagt tcagtacgcg tttattttca acgtagcggc gggtagccag
    31621 gcggctgtct cgctggtcca ggtagtagcc cgttttgtgt ccgtgctgta tgtccaccag
    31681 cagcttcatg ccatgttctt caatcggcag cagggcaggc ggcaactcac cggtgacgag
    31741 gccctgggtc agctccatcc cttctttttt acgtaccgca acatcgctgc gatcgtaaat
    31801 cgcacattcc gggtacagcg tttgcagggc actaattaat gccgcgcgct gatattctgc
    31861 gccagcactc agcagttgca gcaccagaaa attaccgaaa cgatcgatag taatacccgg
    31921 caggccatca gattctccgg cgattaaacg atagctgtcg aggccatctt tttgcgccag
    31981 ccagtcacgc catttttgtg cttgttgcaa acggcgggaa aaaaaagcaa tgtcgataga
    32041 ctcagacggg tcaaacgtcc agacgcgcgc ccggatttgc gaagctggcg aataagcgcc
    32101 gcgtgctaac cattttccct gatgatcaac aatatcgatg gtttcaccga ggctggcttt
    32161 accttccata cgggcaacgg ccccggaaaa gacccacgga tggcgacgaa gtaatgattt
    32221 ttcgcgccct ttggctaaca ctaaacgtac actcataatt tacttttctg tcgatgcaaa
    32281 gaaatgggcg tcattgtccg gagttttcca tggatttgca acgcgtccat taaggataag
    32341 gagagcagca atgtcgaaag tctgcataat tgcctgggtt tacgggcgag ttcagggcgt
    32401 aggatttcgc tacaccacac agtacgaagc gaaaagactg ggcttaaccg ggtacgccaa
    32461 aaatcttgac gatggcagcg tggaagtggt tgcctgcggt gaagaagggc aggtagaaaa
    32521 attaatgcag tggttaaaga gtggcggccc gcgttctgcg cgtgtagaac gggtgctcag
    32581 cgagccgcat catccctcgg gggaattaac cgattttcgt atccgttaaa tacattttac
    32641 cggttttggc aggccagcaa ttttggtggc ttgcttagcc ggacctttcg gaaacagtcg
    32701 gtataaatag cggctgttac ctttttcttc gccaaattta ttcgccatcg cttttaccag
    32761 catacgaatc gccggagaag tattgaattc cagatagaaa tcacgcacaa aacgcaccac
    32821 ttcccagtgt tctggcgaca gcgaaatccc ttcgttctct gcaatcacca ctgccagcgg
    32881 ctcactccac tggctgcttt ctttgagata gccttcggta tccgtttcta tctctttacc
    32941 ttcgaagatc agcataatta ttactacctt aatcagactg ccggcagtgt aacaaaaaac
    33001 aaagccccgc ataaagcgag gctatgaaag tgttagcggg tgagattaat cgcggctggc
    33061 gaagcccaga atgctcagca ggctgacgaa gatgttgtac agcgaaacat acaggctaac
    33121 cgtggcacga atatagttcg tttcaccgcc atgaatgatg ttgctggttt caaacaaaat
    33181 agcgccagag gagatcaaaa tgaagaccgc gctgatcgcc agatgcagag caggcagctg
    33241 caggaagata ttagcaacca taccaatcag caccaccaca atacccgcca tcagcatacc
    33301 gccgaggaac gacatatctt tgcgggtggt cagcacatat gcagagcagc agaagaacac
    33361 taacgccgtt ccgcccagtg ccatagcgat tacgtcaccc attccggcag acagataggt
    33421 attcagaatt ggtccgagga tataacccag aaaaccggta aaggcgaatg cggagataat
    33481 cccggtcggc ttattcgcca ttttataggt caggaacatc aaaccataca tacccaccag
    33541 cgtcagaatc agacccggag atggcagcat cagcaccgta ctggcagttg cggtaatcgc
    33601 cgaaaaggcc agcgtcaggc tcagcagaaa ataggtatta cgcagcactt tatgggtgct
    33661 aagcagtgat gtacggtcat gtgaagaact aacaatacga tccattgagt cactctctat
    33721 gacagatgta attaattaag cagcataatg ataatgcgta agggcaccca gtagttttac
    33781 ccatctttac gcatttgatc tggaacaggt ttaacagcgg attatcaggt cattaagcaa
    33841 atataacgcc ctgagaattt cggcaggcaa aagaaaaagg ggttagcatt tagctaaccc
    33901 cttatcttat ctggcggaag cgcagagatt cgaactctgg aaccctttcg ggtcgccggt
    33961 tttcaagacc gaagaaaaac aaataaaaat cagtgtaata cgtgaaattg ctgaaaagtt
    34021 aatcaaattt aacaccaaga aaaatcaagg tattaggaag ttgctcatag ctagtatacc
    34081 aagtcttttt gccagaaatt tttctggatt gtctacttgc taaccacgag cggcaaatcc
    34141 agtgttggtg tgatttttgt tttacgatcg taaatcaaca cctgattttc tgttttatgc
    34201 ccgctgaaaa tttgtttgtc gcgactgctg ccttcgtaat ctgagatccc cttggctttt
    34261 atgtcgtgaa aattgcaccc gaacggaacg ccaacttttt gctcggctgc gcgtttagcc
    34321 tgattccacc agttattcag cgttttagct atgactttcc cgcctttggt tgtattgatc
    34381 acatattcgc atgtacagga agatacattt cgggctaact ggatcgctgt acgtaatcgc
    34441 ggtgaccatt ccttgatttg tttggtgccg gttttgtttt gctcaatgta gatcccttta
    34501 tccataatat cctgccattt caactcaagc acatcaccga gtcttgccgc acagagatag
    34561 gaaatctcca ttgcaatgcg taactgtgga attgcttccg catatatcgc cgcatactgt
    34621 tcatcggtga tgtaaacagt gcgggcttta agagagaatt ttctgactcc tttgcatggg
    34681 ttattcttca catacccacg ctcatatccc cacccgtata ctcgactcag gcttgccagt
    34741 tcatgatttg cctgggtctt gctctcaagc cctcgtttat ccatgaaaat tcttacctgc
    34801 tcagttttga cattatcagc aagcactttt ccgaatacca tcagcaacgc cttctgatgt
    34861 tgtcgataat ctttttgggt tcggggggcc agttctgtaa atgcagggga gtccataaac
    34921 atgtgccaca atttagctac ggtcattatg ttgtggagtt ttgctttttc cagttcataa
    34981 ttttgccaga ctttagctac gctggttttc cgcactcttc ctagccctat acttcttgta
    35041 cttccttcgg gtttccacac gtaactgtaa ccattcgatc taacccgcgg tggcagtaca
    35101 ttatcttttt tattttttct tggtcttccc attgttcagc gcctcaaaat cgggttcagc
    35161 agaaaccagt tcagatgttt ttggcatcgt tgtcagaccg tgtggaatat ccctgcggag
    35221 aactatgggt tcgtttttag ggccgattac aaatgggatg ccgtgcagtc ttaactggtg
    35281 ttgctgtttt gtgtatcgct cgtatttcgt gatctcttta atctctgctg gagatagagt
    35341 taattcgtac atgtggtcac gtgcctttac agcatgaccg ccgccactat agctggtggg
    35401 cggcgatatg ggttgaacat caaaaatcag cctgactcgg gagcagtttt tgccagatgg
    35461 ctgaaacgta tttcgcctgg taacg
//
LOCUS       DAZKIP010000037        35067 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZKIP010000037 DAZKIP010000000
VERSION     DAZKIP010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35067
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.037"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(146..1087)
                     /gene="fabY"
                     /locus_tag="ACHOUK_002062"
                     /gene_synonym="yiiD"
     CDS             complement(146..1087)
                     /gene="fabY"
                     /locus_tag="ACHOUK_002062"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFP9897519.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1132..1569)
                     /gene="dtd"
                     /locus_tag="ACHOUK_002063"
     CDS             complement(1132..1569)
                     /gene="dtd"
                     /locus_tag="ACHOUK_002063"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFP9897520.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(1566..2438)
                     /gene="yihY"
                     /locus_tag="ACHOUK_002064"
     CDS             complement(1566..2438)
                     /gene="yihY"
                     /locus_tag="ACHOUK_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFP9897521.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2432..3031)
                     /gene="yihX"
                     /locus_tag="ACHOUK_002065"
     CDS             complement(2432..3031)
                     /gene="yihX"
                     /locus_tag="ACHOUK_002065"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFP9897522.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGTWSDLTRIPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3130..3915)
                     /gene="csqR"
                     /locus_tag="ACHOUK_002066"
     CDS             complement(3130..3915)
                     /gene="csqR"
                     /locus_tag="ACHOUK_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9897523.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(3949..4845)
                     /gene="yihV"
                     /locus_tag="ACHOUK_002067"
     CDS             complement(3949..4845)
                     /gene="yihV"
                     /locus_tag="ACHOUK_002067"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HFP9897524.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYKQAKSSQSAIMVDAK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVVLATGGDLAESVRFASGVAASKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5013..5909
                     /gene="yihU"
                     /locus_tag="ACHOUK_002068"
     CDS             5013..5909
                     /gene="yihU"
                     /locus_tag="ACHOUK_002068"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFP9897525.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRSVLFGENGVCESLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFNMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            5933..6811
                     /gene="yihT"
                     /locus_tag="ACHOUK_002069"
     CDS             5933..6811
                     /gene="yihT"
                     /locus_tag="ACHOUK_002069"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HFP9897526.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKILSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLSASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            6827..8068
                     /gene="yihS"
                     /locus_tag="ACHOUK_002070"
     CDS             6827..8068
                     /gene="yihS"
                     /locus_tag="ACHOUK_002070"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HFP9897527.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWDYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGLAPAVAAGLLDINAK"
     gene            8135..9037
                     /gene="yihR"
                     /locus_tag="ACHOUK_002071"
     CDS             8135..9037
                     /gene="yihR"
                     /locus_tag="ACHOUK_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HFP9897528.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHGSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNARTGLSVEIASQNIGTVTAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHANPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEITITHPQQALSVSLCSDQPWVQIYSGEKLQRQGLAVEPMSCPPNAFNS
                     GLDLLLLEPGKTHRLFFNIYGQHN"
     gene            9098..9973
                     /locus_tag="ACHOUK_002072"
     CDS             9098..9973
                     /locus_tag="ACHOUK_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFP9897529.1"
                     /translation="MDFFTLFSPDGVMLETNVVCIVIALMFLYTFVGICAGFGGGLTT
                     MPLVTLMLPVKMATPLSVIVGTATALYATWLSRKETDWRSAMVLIIFSFLGIPVGLYA
                     LSYLPDHFMKVGLGGFLILYSFYSMFIPRLPIYDRSWIAAPIGAVAGALGAAFSTNGP
                     PMVIYGMLRNLGPAAFRGTLNAFFTANNIAVIGGLATSGILTVSIFKLVLFCIPTMIL
                     GSLVGQYVHKRISVKVFRVMVFLLLIASGSMLIKGALGIPAAAALLPPFTLLVILQLV
                     FGKKVAAIRQAEASK"
     gene            10081..12117
                     /gene="yihQ"
                     /locus_tag="ACHOUK_002073"
     CDS             10081..12117
                     /gene="yihQ"
                     /locus_tag="ACHOUK_002073"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HFP9897530.1"
                     /translation="MDTPRPQLPDFEFHQNNDSFTLHFRQRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNKEGVQFLAYI
                     NPYVASDKDLCEEAAKRGYLAKDVAGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGISAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAQTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGETFHGGEITVEAPIGKPPVFYRADSEWAALFASLKNI"
     gene            12163..13548
                     /gene="yihP"
                     /locus_tag="ACHOUK_002074"
     CDS             12163..13548
                     /gene="yihP"
                     /locus_tag="ACHOUK_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9897531.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFIGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPANPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFSGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            13591..14994
                     /gene="yihO"
                     /locus_tag="ACHOUK_002075"
     CDS             13591..14994
                     /gene="yihO"
                     /locus_tag="ACHOUK_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9897532.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLSIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFSLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPLTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCI
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEEKTASVTL"
     gene            15061..15753
                     /gene="ompL"
                     /locus_tag="ACHOUK_002076"
     CDS             15061..15753
                     /gene="ompL"
                     /locus_tag="ACHOUK_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HFP9897533.1"
                     /translation="MKNINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFTPWFNLTVRNRFNHNNYSSTDLNGDLDNNDSYEIGNYWNFIITDKF
                     SYTFEPHYFYNINDFNSGNGTKHHWEITNTFRYRINEHWLPYIELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(15844..17109)
                     /gene="yihN"
                     /locus_tag="ACHOUK_002077"
     CDS             complement(15844..17109)
                     /gene="yihN"
                     /locus_tag="ACHOUK_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9897534.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGITIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLKD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(17211..18191)
                     /gene="yihM"
                     /locus_tag="ACHOUK_002078"
     CDS             complement(17211..18191)
                     /gene="yihM"
                     /locus_tag="ACHOUK_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFP9897535.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVREAYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKELLTHLICLGDEEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPEKHLLDERLRKEKEDAINQINHVRNVLQQIKQEASHLLNH"
     gene            complement(18199..18909)
                     /gene="yihL"
                     /locus_tag="ACHOUK_002079"
     CDS             complement(18199..18909)
                     /gene="yihL"
                     /locus_tag="ACHOUK_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9897536.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(19126..20949)
                     /gene="typA"
                     /locus_tag="ACHOUK_002080"
     CDS             complement(19126..20949)
                     /gene="typA"
                     /locus_tag="ACHOUK_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFP9897537.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            21322..22731
                     /gene="glnA"
                     /locus_tag="ACHOUK_002081"
     CDS             21322..22731
                     /gene="glnA"
                     /locus_tag="ACHOUK_002081"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFP9897538.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            22905..23954
                     /gene="glnL"
                     /locus_tag="ACHOUK_002082"
     CDS             22905..23954
                     /gene="glnL"
                     /locus_tag="ACHOUK_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFP9897539.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            23966..25375
                     /gene="glnG"
                     /locus_tag="ACHOUK_002083"
     CDS             23966..25375
                     /gene="glnG"
                     /locus_tag="ACHOUK_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFP9897540.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            25487..25597
                     /gene="yshB"
                     /locus_tag="ACHOUK_002084"
     CDS             25487..25597
                     /gene="yshB"
                     /locus_tag="ACHOUK_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFP9897541.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(25787..27160)
                     /gene="hemN"
                     /locus_tag="ACHOUK_002085"
     CDS             complement(25787..27160)
                     /gene="hemN"
                     /locus_tag="ACHOUK_002085"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFP9897542.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(27349..27855)
                     /gene="yihI"
                     /locus_tag="ACHOUK_002086"
     CDS             complement(27349..27855)
                     /gene="yihI"
                     /locus_tag="ACHOUK_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFP9897543.1"
                     /translation="MKPSSPSRSKGHAKARRKTREELNQEARDRKRQKKRRGHAPGSR
                     AAGGNNTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELLE
                     TDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQED
                     MMRLLRGN"
     gene            complement(27974..28140)
                     /locus_tag="ACHOUK_002087"
                     /pseudo
     CDS             complement(27974..28140)
                     /locus_tag="ACHOUK_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            28437..29069
                     /gene="yihA"
                     /locus_tag="ACHOUK_002088"
     CDS             28437..29069
                     /gene="yihA"
                     /locus_tag="ACHOUK_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFP9897544.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFNEMQPVEETQDGE"
     gene            complement(29451..32237)
                     /gene="polA"
                     /locus_tag="ACHOUK_002089"
     CDS             complement(29451..32237)
                     /gene="polA"
                     /locus_tag="ACHOUK_002089"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFP9897545.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHRDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            32601..33533
                     /gene="yihG"
                     /locus_tag="ACHOUK_002090"
     CDS             32601..33533
                     /gene="yihG"
                     /locus_tag="ACHOUK_002090"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFP9897546.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            complement(33574..35004)
                     /gene="yihF"
                     /locus_tag="ACHOUK_002091"
     CDS             complement(33574..35004)
                     /gene="yihF"
                     /locus_tag="ACHOUK_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFP9897547.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYQNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDSSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQQSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         8532 a         8955 c         8570 g         9010 t
ORIGIN      
        1 tgctcctgaa cagtgatgta gcaagacacg tatggagcca acgaagaata aaaatttata
       61 gagttcttat tcaatataac cgaaatagcc aaggactaat ataaatgacc tgaatgagag
      121 aggcagcacc gcccctctcc atcagctact cttcttcgtt cccgccctct tcatacgggc
      181 caaatggctt cgcgggcaga acgatatacg tgccttcaaa cactgcaccc ggcgtctcgt
      241 cgccaaagat ttcgacctgc atctgcaccc gtgcttttcg tccgcgcgcc agacggtcga
      301 gatcgccgct taaggcacca aggtcggcta ccgcatgggg tttaccgcta atcggtttgc
      361 tgtagcggat atgcgcatcc gccagaataa tcgttccgcc gaggtggcgt tcgcgcagca
      421 tcagccagat aagcccccaa ccggtgagcg tcgccagtga gaataaactc ccggcaaaca
      481 gcgtatggtg cggattctga ttgccggttt ctggcatggt agtgataaat ttttgcccgg
      541 tatattgctg aatgcgcacg cccatttttt cactaagcgg gatatgttcg taccacgcct
      601 gttgcagctg cgcgcaccag tcgccgcgat gcaaaatatc atccagagtg gcgacgggct
      661 taatcatcaa aaaatggcga atcggcgtgg tggttggcgt ggtgatttct ccctgattaa
      721 caaaccccag cttggcgaaa aactccaccg cgtcttcacg ggcgctacag gtcacgcgct
      781 taacgccttc ctgacgcgcc accgactcca gggtcatcgc catcagcgtg cctaaccctt
      841 tgtcctgcac gtcgggatga acggccataa agcgaatgga cgcttcattg tcggcattaa
      901 tatacagtcg gcctaccgcc accagattac cctgctcgtc gacgaccatc tggtgatgcg
      961 ccatcgcatc ccacgcgtcg cgttccgaac cttttggttg atgcaggggc ttacgcaaca
     1021 tttcccagcg aaactgatag taacgctcta attcttcttc tgtttgtgga acccgaaggt
     1081 gatacatagc gatactctct cttgtttccc gtgacaaccc tggaagctgg ctcatacctg
     1141 caaccagaat gtcacggggc catcattgac cagcgatacc tgcatatccg cagcgaagcg
     1201 tcctgtttgc gtgttcatct cttgctgacg gcagcgttcg acgaaatagt catataacgc
     1261 ctctgcgcga tccggtgatg cacctttgga gaaacttggg cgcatccccc gttcggtatc
     1321 tgcggcgagg gtaaactggg aaaccaccag cacactgccg cccgcctgtt gcacgttgag
     1381 attcatcttg ccttcggcat cgctaaagat gcggtagccg agcacacgct cgcacagacg
     1441 gtttgctttc tgttcgtcgt catccttttc gacacccaat aacaccaaaa gtcccgcacc
     1501 aatttcgccc gtcacttctc cctccacggt gacgctggca cgggttacgc gttgaattaa
     1561 tgcaatcatg gttcgtcgtc ttcttcttgt tcagctgctt gttttagttt gcggtattcc
     1621 ccgagagtga cagtaatttc cgcgccaagc aagacgatac accatgtcca gtagacccaa
     1681 acaaagagaa tggggatcac cgccagcaca ccgtaaatga gctgatatga cgggaacatg
     1741 gtgatataaa gcgcgaaacc tttctttcct gcttcgaaca ggagtgcagc aacaaacgcg
     1801 ccgacaatcg cgtcgcggtt aggtacgcgg atggtaggaa caatgctgta cagcaaccag
     1861 aaggagatcc acgacaacag caacggaaaa atacgcagca cgttgtcgat gacagtattg
     1921 agatcgctcg cccagcgcag ggagagcaaa taggaactga tcgccagact ggcccctgcc
     1981 agcagcggcc ccagcgttaa aatcatccag tacacggcaa acgagtaaat tttgggtcgc
     2041 gctcgtttac tgcgccagat ggtattcaac gcgctatcga tggagtacat caacaataac
     2101 gcggtgacga tcagcccgca cgccccaacg gcggtcatct tgttggaatt ggcaacaaat
     2161 tgttcgatat accgctgaat aacatcgcca gtagcaggca gaaagttggc aaaaataaag
     2221 tgacgtaact gaatgctgac gtcggaaaac atgggaaaag cggcgaaaag cgcaaaaaca
     2281 acggcaacca gcggcactaa tgagagcaac gacacatagg caaggttacc tgccagggtt
     2341 gtcatattgt cctcatcaat gcgttgccag agtagtttta gccaggccca tagtggacgg
     2401 gtacggtgcc tggctttgtc ctgaatggtt tttagcataa caccttcgcg aaatagtccg
     2461 ggatggtggt tttatctttc accagaatac tggtaatgcc cagctgatta gctccttcta
     2521 tattatcggc gttatcgtcg aaaaagaccg tatcgctggg tgaaaaacct tccgcctgca
     2581 aaacatgctg gtaaattcga gcttcaggtt tgcgcatccc cagatcttgc gacagataga
     2641 tatggtcagc agcatcacga atttccgggt attcttccgg ccagaaggtg gtatgcaggc
     2701 ggttagtatt ggaaagcacc accacgcgat gcccctgctc acgcagttta tgcatgatgg
     2761 cgatcacttc cgggcgcagc gcaacaaaca ccgcctgcca gccgtgagag aactgctcgt
     2821 agcttagcgg tagagccatc tcatgacaca gcgcctctgc gaacgcttcg tcgctaattt
     2881 ccccacgctc atgctgatga aacgcctccc ccatatgaaa actcttctta agcgtagcca
     2941 gcggaatacg cgttaaatcg ctccaggttc ccagcacacg gttaaagtcg atatcgacaa
     3001 tcacattacc taaatcaaag atatagagca tttttgcctc ctttcgcgcc acaagaaatt
     3061 aactgtagcg ggaaaggatg ggtttggcta tgtggcaagg tatgaagcca ggtcaacagt
     3121 cacaaaaatc tacgcgtctt cctggggaag gatgatttca atttggctgt cttgtaagcg
     3181 tgattttagc gcagcgggcg gcagctcgtc ggtaaagagc gcggtgacct gtgccacgtt
     3241 accaatttca accgccgccg aagcatgata cttagtgtga tcggcaacca gcaggatatt
     3301 tctcgcgtgc gccatcatcg ttttcaccac gttagcttcg tttacatcaa actccatcaa
     3361 cgcgccatcg ctctcaatcg ccccaacgct tgttaccaga taatcagcac gaaaatcggc
     3421 cacaaaggac gccgctgaag ggccaatgat cccgctatta tgagagcgca acgtaccgcc
     3481 gggtaccatc acttcaaagc gcgggttgtg gtaaagaata tgcgccacac gcaggctgtt
     3541 ggtgattatc cgcaaatgat tatggttaag taacgcccgg gcaacatgct caacggtcgt
     3601 accaatggtg ataaatattg ttgaaccatc aggaatatag tctgccaccg cttcggcaat
     3661 cgcttttttt tcctcggttt gcgaaacctc acgctgctcg aacgccgtat taacgacgct
     3721 ggaagcccga cccgcgccac cgtgatggcg cgtaatcagg ccttgctcgc ttaatttacg
     3781 aatatcccgg cgcaccgtct gcgtggagac atccagcaga tttgccagct catcaatatt
     3841 catataccca cgctcggcga tcagcatgag gagttggtcg tgccgcgggt taccggttag
     3901 ttcggtaagg ctcatgaaaa ttcctcgaaa aaccatcacc ctggcatttt atacaaaaag
     3961 tgacaaaaaa gatcgggttt gatcacagtc agggatcccg gctcgtccac cgggacgcgt
     4021 gcatttcgac gccgctacac cgctggcgaa gcggactgac tccgctaaat ccccacctgt
     4081 tgccagcacc accgccaatg cgccgtgaaa aacatcaccc gcacctgtgg tatctaccac
     4141 atcaactttg aaggccggtt gatgttgacg cccgccattt tccagccagt cacagcctgc
     4201 gctgccctgg gtcacataga catgaccatt tgtgagcgtt tgtgcctgtt ttagcgcact
     4261 ggccatctct ttcacgcccg ttaagcgcgc cagacccggt tctgaaaagg ccgcatgatc
     4321 gcttaatgcc accagctcgc tgatatcctg cggcgtaata tcaccgtcca gaacggtcat
     4381 cacgcccgcc tgccgggcca gagtgaaggc ttttttagcg ccgtcgtgcc agcgtacatc
     4441 tgccagcaca acatcccact gagagaaatc aatttcctcc aaccactctg cgtcaggcag
     4501 caggtccggg ctggggtagt taatgattat ccgctcgcct ttggcatcca ccatgatggc
     4561 ggattgcgac gatttcgcct gtttataccg tttggtgtag cgggtgttaa cgccccagga
     4621 ttccagctct gccagcaggc tgttgccggt gtcgtcatca ccgacgcgcc caataaaatc
     4681 aacctgcgct cccagtcttg ccgccgcaac cgctgccgtc gccgctggcc cgccaccaac
     4741 ttccgtataa tttctcgcca cgtatttacc gctctccgtc ggtaaccctt ccacgtaata
     4801 gatgcgatcc atcacggtta tacctacaca agcaacacga atcatggtca ttccttaaac
     4861 attttgggat gcgttcattt taatttcacc aattgtttaa aaagtgacgg ctgtcaattt
     4921 tttgactata aattacaaat acgatcaaaa acagacaatc aaagcgtgtc ataaatgaca
     4981 aaaagtgaca tcactgtatt caggagaagg ttatggcagc aatcgcgttt atcggtttag
     5041 gacaaatggg ttcgccaatg gcgagcaatt tattgcagca agggcaccaa cttcgcgtct
     5101 ttgatgtgaa tgccgaggct gtgcggcatc tggtagacaa aggcgcgact cccgccgcca
     5161 acccggcgca ggccgctaaa gatgccgaat ttatcattac catgttgccg aatggcgatc
     5221 tggtgcgcag cgtgttgttc ggtgaaaacg gcgtttgcga aagcttatct accgatgcgc
     5281 tggtcattga tatgtcgacc atccatccgc tgcaaaccga taaattgatt gccgatatgc
     5341 aagccaaagg cttcaacatg atggatgttc cggtaggccg tacttctgca aatgccatta
     5401 ccggtactct gttactgctg gctggcggca ccgctgaaca agttgaacgt gccacgccga
     5461 tcctgatggc gatgggcagt gagttgatca acgcaggcgg tccgggcatg gggatccgcg
     5521 ttaagctcat caacaactat atgagcatcg cgctcaatgc gctttcggca gaagctgccg
     5581 ttttgtgcga agccctgaat cttcccttcg atgttgccgt caaagtgatg agcggtaccg
     5641 ccgccggtaa aggccacttc accacttcct ggccgaacaa agtcctcagc ggggatcttt
     5701 ctcccgcctt catgatcgat cttgcccata aggatcttgg catcgccctt gatgtcgcca
     5761 accagctgca tgtgccaatg ccgctggggg ccgcctcacg ggaggtttat agccaggcgc
     5821 gcgcagcggg tcgcggtcgc caggactggt ccgccattct ggaacaggtc cgtgtcagtg
     5881 ccgggatgac tgccaaagta aaaatgtaac gactggataa aggaataaat gaatgaataa
     5941 gtacaccatc aacgacatta cgcgcgcatc gggcggtttt gccatgctgg cggtcgatca
     6001 gcgcgaagcc atgcgcatga tgtttgccgc ggctggggca cccgctccgg tagccgatag
     6061 cgttttaact gatttcaaag ttaacgcggc gaagatcctc tcgccttatg cctcggcgat
     6121 tctggtagat caacaattct gttaccgcca ggtggttgag caaaacgcga ttgccaaaag
     6181 ttgcgccatg attgtcgccg ccgatgagtt cattcctggc aacggtattc cggtagatag
     6241 cgtggtcatt gaccgcaaaa tcaatccgct acagatcaaa caagacggtg gcaaagcctt
     6301 aaaactgctg gtgctgtggc gtagtgatga agatgcgcag caacgcctgg atatggtgaa
     6361 agagtttaac gaactgtgcc actcacacgg cctggtaagc attattgagc cagtcgtccg
     6421 cccaccacgt cgtggcgata aattcgatcg cgaacaagcg atcatcgatg ccgccaaaga
     6481 gctgggcgac agtggcgctg acctctacaa agttgaaatg ccgctttacg gtaaaggtcc
     6541 gcaacaagag cttctcagtg cttcacaacg tctgaatgac catatcaata tgccgtgggt
     6601 gatcctctct tccggtgtcg acgaaaaact gttcccgcgt gccgtacgcg tggcaatgac
     6661 agcgggtgca tcgggcttcc tggctggtcg tgctgtctgg gcatcggtcg tcggtttacc
     6721 agacaacgag ctgatgctgc gtgacgtttg cgcaccgaaa ttacaacaac ttggcgatat
     6781 cgtcgacgaa atgatggcta aacgccgtta aaatggggat acaaaaatga aatggtttaa
     6841 cactttaagt cacaaccgtt ggctggaaca ggaaaccgat cgcatctttg attttggtaa
     6901 aaattccgta gtaccgaccg gatttggctg gttaggcaat aaagggcaaa tcaaagaaga
     6961 gatgggcacc catctgtgga tcacagctcg tatgttgcac gtttattccg ttgctgcggc
     7021 gatgggtcga cctggcgctt actcgttggt tgatcacggt attaaagcca tgaacggcgc
     7081 actgcgcgat aaaaaatatg gcggctggta tgcctgtgtg aatgacgagg gcgtggtgga
     7141 tgcctccaaa cagggctatc aacatttctt tgctctgctg ggtgctgcca gcgccgtcac
     7201 aacgggtcac ccggaagcgc gcaagctgct cgattacacc attgaaatta tcgagaaata
     7261 cttctggagc gaagaagagc agatgtgcct ggagtcctgg gacgaagcct tcagcaaaac
     7321 cgaagagtac cgcggcggca atgccaatat gcacgcagtg gaagctttct taattgttta
     7381 tgacgtaacg catgacaaaa aatggctgga tcgcgcgatt cgcgtggctt ccgtgattat
     7441 ccacgacgtc gcaagaaata atcattatcg cgttaacgaa cacttcgata cccagtggaa
     7501 tccgctgccg gattacaaca aagataaccc ggcgcaccgc ttccgcgcgt tcggaggtac
     7561 accaggccac tggatcgaat ggggccgttt aatgctgcac atccacgcgg ccctggaagc
     7621 ccgttgcgaa caaccgccag catggctgct agaagatgcc aaaggtctgt ttaacgccac
     7681 cgtgcgtgat gcctgggcac ccgatggtgc ggacgggatt gtttataccg ttgactggga
     7741 aggaaaaccg gtggtccgcg aacgtgtgcg ttggcctatc gtcgaagcga tgggtacggc
     7801 ctacgcgctc tacaccgtca ccggcgatcg ccagtacgaa acctggtatc agacatggtg
     7861 ggactactgc atcaaatacc tgatggacta cgaaaatggt tcatggtggc aggagctgga
     7921 tgcggacaat aaggtcacca ccaaagtctg ggacggcaaa caggatattt atcacctgct
     7981 gcattgcctg gtgatcccgc gtatcccgtt agcgccaggc ctggctccgg cagttgcggc
     8041 gggtctgctg gatattaacg cgaaataact gactaaaaaa acgacatatc aggctgcacg
     8101 ccgggtatgt cgttattcaa taaaggtacc tcgtatgcaa atcaccaaca tgcattgcag
     8161 tggacagacc gtttcacttg ccgcggggga ttatcacgcg accattgtga ccgtcggtgc
     8221 aggtctggct gaattaacct ttcagggctg tcatttagtt attccacata aacccgaaga
     8281 gatgccgctg gcacatctgg gcaaagtcct tattccgtgg ccgaaccgga tcgccaacgg
     8341 ctgttatcgc tatcaggggc aggaatatca gttacccatt aatgaacatg gttcaaaagc
     8401 cgctattcat gggctgctcg cctggcgtga ctggcaaatc agcgaattaa ccgccaccag
     8461 cgttaccctg acagcttttc tgccgcccag ttacggctat ccgtttatgc tggcttcgca
     8521 ggttgtttat tcgctcaatg cacgcacggg tctttccgtt gagatcgcaa gtcagaatat
     8581 tggtactgtg accgcaccct atggtgtggg tattcatcct tatttaacct gcaacttaac
     8641 atctgttgat gaatatctgt tccagcttcc ggcaaaccag gtttatgccg tcgatgagca
     8701 cgctaatccg acaacgcttc accacgttga tgagctggat ttaaatttca cgcaggcgaa
     8761 aaaaatagcc gcgacaaaaa tcgaccacac ctttaaaacc gctaatgatc tgtgggaaat
     8821 aaccattacc catccacaac aggcgctttc ggttagcctt tgttccgatc aaccctgggt
     8881 acaaatttat agcggtgaaa aattacagcg tcagggactg gcagttgaac ctatgagttg
     8941 cccgcctaac gcctttaatt caggtctcga cttattatta ctggaaccag ggaaaacgca
     9001 ccgcctgttt tttaatattt acgggcaaca caattaattt tattaagaaa agaacgagat
     9061 cattgtttag agttgtattt acacaacagg aaatattatg gactttttta cgttattttc
     9121 accggatggg gtcatgctgg aaacaaacgt ggtatgtatc gttatcgcgt taatgtttct
     9181 ttataccttt gtggggatat gcgcgggatt tggcggtggt ttaaccacta tgccgctcgt
     9241 cacgttaatg ctccccgtaa aaatggcaac accactctcg gtaattgtcg gcaccgcgac
     9301 cgccttgtac gccacctggc tgtcacgtaa agaaacggat tggcgatcgg caatggtact
     9361 gattattttc tcgtttctgg gaatacccgt cggtttatat gcactgtcgt atttacccga
     9421 ccattttatg aaagtgggct taggcggatt ccttatcctg tactcctttt acagtatgtt
     9481 tattccacgc ctgccgattt atgaccgtag ctggatcgcc gcacctatcg gtgcagtagc
     9541 aggcgcatta ggcgcagctt tctcaaccaa cggaccaccg atggttatct acggtatgct
     9601 gcgtaactta ggcccggcgg catttcgtgg cactctgaac gctttcttca ccgcaaacaa
     9661 catcgcggtg atcggcgggc tggcgaccag cggaattttg actgtttcga tctttaaact
     9721 ggtgctgttt tgtattccca ccatgatcct cggttcactg gttggacaat atgtgcataa
     9781 acgtatttca gtcaaagtgt tccgtgtgat ggtcttttta ttgctgattg cctccggttc
     9841 catgttgata aaaggcgcgc tgggaattcc cgccgctgcc gcacttctac ctccgtttac
     9901 cttgttagtt atactgcaac tggtatttgg taaaaaagtg gcggcaatcc gtcaggctga
     9961 agcaagtaaa taatcactta tgcgggttta aatttatatc cgcacacatt ctttaattaa
    10021 ttaaacctgt aataacagga tggcatgaaa tattagcttt atactctatg gagattattt
    10081 atggatacgc cacgcccaca attaccagat tttgaatttc atcagaataa cgacagtttt
    10141 accctacatt ttcgacaacg tcttatttta acccatagca aagataatcc ttgtttatgg
    10201 attggctcag gtatagcgga catcgatatg ttccgcggca atttcagcat taaagataaa
    10261 ctacaggaga aaattgcgct taccgacgcc atcgtcagcc agtcaccgga tggttggtta
    10321 atccatttca gtcgtggttc tgacattagc gccacgctga atatctctgc cgacgatcag
    10381 ggccgtttat tgctggaact acaaaacgac aaccttaacc acaaccgcat ctggctgcgc
    10441 cttgccgctc aaccagagga ccatatctac ggctgcggcg aacagttttc ctacttcgat
    10501 ctgcgcggca aaccgttccc gctatggacc agtgaacaag gcgttggtcg caacaaacaa
    10561 acctatgtca cctggcaggc cgactgcaaa gaaaatgcgg gcggcgacta ttactggact
    10621 ttcttcccac agcctacgtt tgtcagcacg cagaagtatt attgccatgt tgataacagt
    10681 tgctatatga acttcgactt tagtgccccg gaataccatg aactggcgct gtgggaagac
    10741 aaagcaacgc tgcgttttga atgtgctgac acatacatct ccctgctgga aaaattaacc
    10801 gccctgctgg gacgccagcc agaactgccc gactggattt atgacggagt aacgctcggc
    10861 attcagggcg ggacggaagt gtgccagaag aaactggaca ccatgcgtaa cgcgggcgtg
    10921 aaggtcaacg gcatctgggc gcaggactgg tccggtattc gtatgacctc ttttggcaaa
    10981 cgcgtgatgt ggaactggaa gtggaacagc gaaaactacc cgcaactgga ttcgcgtatc
    11041 aagcagtgga acaaagaggg cgtgcagttc cttgcctata tcaacccgta tgttgccagt
    11101 gataaagatc tctgcgaaga ggcggcaaag cgcggttatc tggcaaaaga tgtcgccggt
    11161 ggcgactatc tggtggagtt tggcgagttc tacggcggcg ttgtcgatct caccaatccg
    11221 gaagcctacg cctggttcaa ggaagttatc aaaaagaaca tgattgaact cggctgcggc
    11281 ggctggatgg ctgacttcgg cgagtatctg cccaccgaca cgtacctgca taacggcatc
    11341 agcgcggaaa ttatgcataa cgcctggccc gcgctgtggg cgaaatgtaa ctacgaagcc
    11401 cttgaagaaa cgggcaagct cggcgagatc ctcttcttta tgcgcgccgg ttctaccggt
    11461 agccagaaat actccaccat gatgtgggcg ggtgaccaga acgtggactg gagtctggac
    11521 gatggcctgg cgtcggttgt cccggcggcg ctatctctgg caatgaccgg acatggcctg
    11581 catcacagcg acattggcgg ttataccacc ctgtttgaga tgaagcgcag caaagagctg
    11641 ctgctgcgct ggtgcgattt cagcgccttc acgccgatga tgcgcaccca cgaaggtaac
    11701 cgtcctggcg acaactggca gtttgacggc gacgcggaaa ccatcgccca tttcgcccgt
    11761 atgaccaccg tcttcaccac cctgaaacct tacctgaaag aggccgtcgc gctgaatgcg
    11821 aagtccggcc tgccggttat gcgcccgctg ttcctgcatt acgaagacga cgcgcagacc
    11881 tattccctga aatatcagta cctgttaggt cgcgacattc tggttgctcc ggtacacgaa
    11941 gaaggtcgca gcgactggac gctctatctg ccagaggata actgggtcca cgcctggacc
    12001 ggagaaacct tccacggtgg agaaataact gttgaagcgc ccatcggcaa gccgccggtc
    12061 ttttatcgcg ccgacagcga atgggctgca ctgttcgcct cgttaaaaaa catctaatca
    12121 tgcccgcccg tggggaaact cacgggcaaa tggagaataa cgatgagtca catcacaacg
    12181 gaagatccgg caactttgcg cctgcccttt aaagagaaac tctcttacgg tatcggcgat
    12241 ctggcctcta acatcctgct ggatattggt acgctttatc ttttgaagtt ttataccgac
    12301 gttctggggc tacctggcac ctatggcggc attatctttt tgatctcgaa attctttacc
    12361 gcctttaccg atatgggaac cggcatcatg ttggattccc ggcgtaagat tggcccgaaa
    12421 ggtaagttcc gccctttcat tttgtacgcg tcattcccgg tcaccttatt ggcaatcgct
    12481 aactttatcg gcacaccgtt cgatgtcact ggtaaaacgg tgatggccac tattctgttt
    12541 atgctctacg ggctgttttt cagcatgatg aactgctcct atggcgcgat ggtgcctgct
    12601 attaccaaaa accccaacga gcgcgcatca ctggcggcat ggcgtcaggg aggcgctacg
    12661 ctgggcctgc tgctgtgcac ggtgggattc gtgccggtta tgaatcttat cgaaggtaat
    12721 cagcaacttg gctatatctt cgccgccacg ctgttttcac tgttcggcct gctgtttatg
    12781 tggatctgct actcgggcgt gaaagagcgt tatgtcgaaa cccaaccagc caatccggcg
    12841 caaaagcctg gcctgctgca atctttccgc gcaattgccg gtaaccgccc actgttcatt
    12901 ctgtgcattg ccaacctctg cactttaggg gcgtttaacg tcaagctcgc catccaggtc
    12961 tattacaccc agtacgtact taacgatccc atcctgttgt catatatggg atttttcagc
    13021 atgggctgta ttttcatcgg tgtgttcctg atgcctggcg cagtcaggcg ttttggtaag
    13081 aagaaggtct atatcggcgg cctactgatt tgggtgctgg gcgatctgct caactatttc
    13141 ttcagcggcg gttcggtcag cttcgtggcg ttctcctgcc tggcattctt cggctcagcg
    13201 tttgttaaca gcctgaactg ggcgctggtt tccgacaccg tcgagtacgg cgagtggcgt
    13261 accggcgttc gttcggaagg aacggtctac accggcttca ccttctttcg caaagtgtct
    13321 caggcgctgg ctggtttctt ccccggctgg atgctgacgc aaatcggtta tgtgccgaac
    13381 gtggcgcagg ctgaccacac cattgaaggg ttgcgccagc tgatcttcat ctacccaagc
    13441 gcactggcgg tagtcaccat tgtggcgatg ggctgcttct acagcctgaa cgagaagatg
    13501 tatgtccgca ttgttgaaga aatagaagcc cgtaaacgca cggcgtaata ataataaaca
    13561 atgccctgcg gggcgttata aggagtgatt atgtctgacc ataatccact gacattaaaa
    13621 ctgaatctgc gggaaaaaat cgcctatggt atgggcgacg ttggttcgaa tttaatgctt
    13681 agcatcggta ctctgtatct cctcaaattt tataccgatg aattagggat gccagcttac
    13741 tatggcggga ttatctttct ggtggcgaag ttttttaccg cgtttaccga tatgctcacc
    13801 ggatttttac tcgattcgcg gaaaaatatt gggcctaaag gcaaatttcg tccctttatt
    13861 ttatacgcag cggttccggc ggcgttaatt gcaacgctcc agtttatcgc caccactttt
    13921 agtttgccgg ttaaaacgac gattgccacc gcgctgttta tgatgtttgg actttcatac
    13981 agtctgatga actgctcgta tggtgcgatg atcccggcca ttactaaaaa cccgaatgag
    14041 cgcgcgcagc tcgcggctta ccgccagggt ggtgcaacca tagggctatt gatttgtacc
    14101 gtggcgttta ttccgttgca gtcgcttttt tctgactcaa ccgtcggtta tgcctgtgcg
    14161 gcactgatgt tctccattgg tggctttatt tttatgatgc tgtgctacag aggcgtcaaa
    14221 gagcattatg tggacacagc accaaccgga cataaagcca gtattctcaa atctttttgc
    14281 gcgatatttc ggaatccgcc attgctggtt ttatgcattg ctaacttgtg taccctggcg
    14341 gcatttaata tcaaactggc gattcaggtc tattacaccc agtatgtgct gaatgatatt
    14401 aatttattgt cgtggatggg attcttcagt atgggatgca tcctcgtcgg cgtattactg
    14461 gtgccattaa ctgtaaaatg ttttggtaaa aaacaggttt atttagctgg catggtgcta
    14521 tgggcggttg gtgatatcct gaattatttc tggggaagta actctttcac tttcgtcatg
    14581 ttctcttgta tcgccttttt tggcacggcc tttgtaaaca gcctgaactg ggcactggtg
    14641 ccagataccg tagattacgg tgaatggaaa accggtattc gtgccgaagg ttctgtttat
    14701 accggttata ccttctttcg taaaatttct gccgcacttg ctggcttctt gccaggcatt
    14761 atgctgacgc aaatcggtta tgttcccaac atcgcgcaaa gtgatgcgac attacagggt
    14821 ttgcgtcagc tcatttttat ctggccttgt gcactggcaa ttattgctgc attgacgatg
    14881 ggattctttt acacactcaa tgaaaaacgg tttgcgttga ttattgagga aatcaaccaa
    14941 cgcaaaaata aagagatggc gacagaagaa aaaacggctt ccgtaacgtt ataaatgtca
    15001 atagccgcta tttccatcct ggtgggtggc ggcctcccta cgtttaaaaa tggacttatt
    15061 atgaaaaata ttaatgcaat aattttatta tcttctctga cctctgcctc agtattcgct
    15121 ggcgcatacg tcgaaaacag ggaggcatat aatctcgcct ccgatcaggg cgaggttatg
    15181 ctacgtgtgg gttataactt cgatatgggc gcgggtatta tgttaaccaa tacctacacc
    15241 tttcagcgag aagatgaact aaaacatgga tataacgaaa tcgaaggttg gtatccgtta
    15301 tttaaaccaa ccgataaatt aaccatccag cccggtggct taattaatga taagagtatc
    15361 ggttcgggtg gtgctgttta tctggatgtt aactacaaat ttactccgtg gtttaacctg
    15421 acagtgcgca accgttttaa ccataataac tacagctcaa ccgatttaaa cggtgatctg
    15481 gataataatg acagctacga gattggcaac tactggaact ttattattac cgataagttc
    15541 tcatatacct ttgagccgca ttatttttat aacattaatg atttcaatag cggcaatggc
    15601 acgaagcatc actgggaaat cactaacacc ttccgttacc gcattaacga gcactggctg
    15661 ccctatattg aattgcgttg gttagatcgc aacgtggaac cgtaccaccg cgagcaaaac
    15721 cagattcgta tcgggacgaa gtatttcttc tgatgtgaaa ctgccggatg cggcgtaaac
    15781 gccttatccg gcctacggtt gatacggttt tcgttggccg gattaagtac atccggccat
    15841 taattaagct tcagccgcca gcatctcttt cttacgttgg ctaatacgct taaccagcat
    15901 tacggaaacc accgcaccac agaatgcgaa gcaggccata atgccaaaca cgattttgta
    15961 gccaataatc cccggattta aatccagaat gtagccatac agactgaagc agaacatcgc
    16021 cggggcgtaa ccaataaagc tacccaacgc catcgccgcg cctgttttat tttcagcaat
    16081 ttttgcttcg ccgataggtg caaaaaatac ggctcgctgt gtaaagacta tcgcgccaaa
    16141 gcccagcgta catgccatcc ctaaatagac cggcatactt tcgtgcggca gcataatcaa
    16201 cagtacgagc gcagcggtac tgatgataaa ggtgtagcat agatatttac tcggcgattt
    16261 caggatttta tctgaaatca tgccaccaat cggtccacca atcattttca gacagtattg
    16321 gttaatgatg ccgtaagccc ccaccagcgc aacgggcaat agatagatgt ttttcaggaa
    16381 tggaatgaag aatgtcaggc cacagtaaac cgcgtaaacg aagaagacgt tgaaagcgat
    16441 aagccagata gttttgtctt tcagcaccga ggtcatactg gtgttttgcg atgccccgtc
    16501 ttcttttttc acctcaacgg acggtgcctc ttctttgtca ttcaggacaa agaaaatagt
    16561 aatgcctacg gcaatcacaa taagggaata gaaccagatg cctgctttaa aacctaataa
    16621 gccactgcca aaccaggtaa atactgccaa cgcagaaaat gccaccacgg tatcgacaat
    16681 gccacgccct gtttcgaaga agccaaacaa ccgaccttgt tgttcgctat tgcccaatcg
    16741 acttaccgac ttgagcaaga ccggccagtt catcatgtcg caagtaacgc caaatagcgc
    16801 ccagacaaag aggatgcccc aataccccgg cattgtcgtc aaataaacac ccagtaatcc
    16861 tgtcgcaatg agtgagaatg acatggtgta tctgcgcggt agtttatcgg caaaataaat
    16921 agacagaaaa aagcccactg tggtaacaaa tgagtttacc gacatagcat taccaatttg
    16981 accattggtc aaatggaaat attcctgcat cgggatataa aacgcatctt tcagcgacgg
    17041 taatttataa attgtaccgc cacacagtgt taatagacta aataacgccc atttcttttt
    17101 cgtgagcata acatccccta agcgatttta aaaatcactg ttttaaaggc aataagatac
    17161 cgaccattct tatggaatgg ttttattaac tgcgtatgag ttaaaacttg ttagtggttc
    17221 agaagatggc tcgcctcttg tttgatttgt tgtagtacgt tacgcacatg atttatctga
    17281 ttaattgcat cttctttttc tttacgtaac cgctcatcca gcaaatgctt ttctggcaat
    17341 ggtgtttcac tcatctggcg ataagggatc cacggattat catcttcgtc ttcaaagcgg
    17401 aacgctggag cataataatc aacctcttct tccaggccat atgccgtcac ctgcggttct
    17461 tcatcgccga ggcagataag gtgtgttaat aactctttga acggcatatc tccctgacct
    17521 gaaatacagg ctttatgacc caggccacct tgttctttaa cgatcaaggc atctttgata
    17581 tggacctggg taatatgcgg tgccatcgtt tttaaggcgt ctatgggatg ctcatttgca
    17641 ttgatcatat tcgcaaaatc aaacagtaag gaaagagact ccatctcact ttctttgacc
    17701 agcgacacca actcatgact ttttaaatct tcatgctgtt cgatcgtaaa agtcaggccg
    17761 ctatcctgat acgcttcccg cacataggca atgtcgttag cgatgatcga taatacgtcg
    17821 cgcaaattac cttcataacg tggataaaaa cgtacggacg atgccccagt tttcaacgcg
    17881 atggcgacgg cttcgtcaat agatgcctta tctgaggcgc tggtttcaat atgaatatca
    17941 aggttcagac ggcgggcttt atccccaaag gcagagagtt ctttatcgtc catattacca
    18001 agagaaaaac gctcgccatc cagaacatgg attttgacgc cacgtagatt attttcactt
    18061 gcgatatcaa gtaaatcagc aggcaacact ctttccagcc gcatatttaa atgaaaggca
    18121 taagcatgta aataaagagg aagagtgtcg acacgttgta gaatctttct tgcgttattt
    18181 attgtcacca taaaacacct acagattaat gatgaggtct acggcctcaa atcgccaata
    18241 ttcaatatct atctggacaa tattgccttg ctggtcggcg cgatgctttt caattaagat
    18301 cgctggcata cccgcagaac cgccaagata cttgctgata tcgcccggca ttctgacggg
    18361 tttgaaaatc aattttttta ctaccgtctc tttttggtag cacttttccc agactgcaga
    18421 aaatgagtgg ttttcaagtt gttcaataaa acccggagca acttcaggat taatatatgt
    18481 ttcgtgatag aaaactttat gtccttccag cgccccccag ccggtgatgc gatagagttc
    18541 agttgatggt gtcactgcaa tcagtggcgc gagcgtctcg ggaatatcgc tggtacggtt
    18601 tttctcggta aacccccagg aaggctctcg cccttgctca attgcggccc gctgaaagct
    18661 cgccgacagc tccggactgt aattaaatcg cggctgggtc acgaaccacc ccttacgatc
    18721 cttacggaag attttggatt ctgcctcgag gttcaacaac gcctggcgca gcgtcatacg
    18781 tttaatgccc agcagttctc ccagctctct ttctgaaggg agttttccac ccggcgtggt
    18841 gatgccatct ttcaaccacc gatccagttt ctcttttgca ttttctacgg tggattgatt
    18901 ctccgccatt gtttaatcct tttggtttaa accagatgaa gcatcttacg cttacgccaa
    18961 aaaagaaagc aattaacggc gtatgacttt gcagataatt gtgacaaagc tctctattga
    19021 cgtaaccaca gacttataag ggagatactc gcattggtgt gcgctcacga caggcaaaaa
    19081 aaaacctgcc atcgctggca ggttttttat gactaaaaaa cgaaattaat cgtctttcgg
    19141 tgcgcggttg gcgcgacgac gatcgttttc cgtcaggtga cgtttacgaa tacggataga
    19201 ggtcggagtc acttctacca gttcgtcatc atcgatgaac tccagagctt gttccagagt
    19261 catgcggata ggcggaacca gaacaacggc ttcgtcagta ccggaagcac gcatgttggt
    19321 cagtttctta ccggtcaggc agtttacagt caggtcgtta gagcggctat gaataccgat
    19381 aatctgacct tcgtaaactt ctgcaccgtg accgaggaac agcttaccgc gatcctgcaa
    19441 accgaacagc gcgaacgcga ccgctttacc ctgaccgtta gagatcagta cgccgttctg
    19501 acgctgaccc acttcacccg gacgcacgtc gtcgtagtgg ctgaaggtgg agtacagcag
    19561 acccgtaccg gaagtcatgg tcatgaactc agaacggaag ccaatcagac cacggcttgg
    19621 gatcacgtag tcgagacgta cgcggccttt accgtctgga ttcatgtttt tcaggtcgcc
    19681 tttacgttcg cccagcgcct gcattacaga accctgatgc tgttcttcaa cgtccagagt
    19741 cacgttttca tacggctctt gtttacgacc gtcgatttca cggaagataa ctttcggacg
    19801 ggataccgcc agttcgaaac cttcacgacg catgttttcg atcagaacag acaggtgcag
    19861 ttcgccacga ccagaaacgc ggaacgcatc ggcgtcttcg gtttcttcta cgcgcagcgc
    19921 aacgttgtgt accagttctt tgttcagacg atccaggatc tgacgagacg ttacgaactt
    19981 accttcttta ccgcagaacg gcgaggtgtt aacgcagaag aacatagaaa cggtcggctc
    20041 atcaacggag agtgccggca gcgcttcaac gttttgcgtg tcgcaaacgg tgtcagaaat
    20101 gttcagttcg cccaggcccg tgatcgccac gatatcgcca gcttccgcca gatcggtttc
    20161 gatacgttcc agaccgaggt ggcccagcac tttaccaact ttcgcgttgc gggttttgcc
    20221 ttcgctatcg atgatagtaa cctgctggtt cggcttcact ttaccgcgct tgatgcggcc
    20281 aatgccgata acgccaacat agctgttgta atcgagctga gaaatctgca tctggaacgg
    20341 accgtcaagg tcaacgtccg gcgcaggaac gtggtcaaca atcgcctggt acagcggggt
    20401 catgtcttcc gccatatctt cgtggtccag acccgcgata ccgttcagcg cagaagcgta
    20461 aacgatcggg aagtccagct gctcgtcggt cgcgtcgagg ttaacgaaca gatcgaatac
    20521 ctgatccaca acccaatcag gacgcgcgcc agggcggtca actttgttga taacaacaat
    20581 cggcttcagg ccgtaagcaa acgctttttt ggttacgaag cgcgtttgcg gcatcgggcc
    20641 gtcaaatgcg tcaaccacca gcagcactga gtctaccatg gacattacac gttcaacttc
    20701 accaccgaag tcggcgtgcc ccggggtatc aacgatgttg atacggtaat cattccattt
    20761 gatagcggtg tttttcgcga ggatggtaat cccacgctct ttctccaaat cgttggagtc
    20821 catcacgcgc tcttgggttt cggcacgaga gtcgaacgta ccggattgtt ggagcagctt
    20881 gtctaccagg gtggttttac catggtctac gtgcgcgatg atggcgatat tacgcaattt
    20941 ttcgatcaca actttgcctc aggcattaga aatagcgcgt tattgtacac ggattaatcg
    21001 cactacaaaa caggatcaca aacatcctcc gcaaacaagt attgcagagt ccctttgtga
    21061 tcgctttcac ggagcataaa aagggttatc caaaggtcat tgcaccaata tggtgcttaa
    21121 tgtttccatt gaagcactat attggtgcaa cattcacatc atggtgcagc ccttttgcac
    21181 gatgttaagc atgataacgc cttttagggg caatttaaaa gttggcacag ttttcgcttt
    21241 atctttttta cggcgacacg gccaaaataa ttgcagattt cgttaccacg acgacaatga
    21301 ccaatccagg agagttaaag tatgtccgct gaacacgtac tgacgatgct gaacgagcac
    21361 gaagtgaagt ttgttgattt gcgcttcacc gataccaaag gtaaagaaca gcacgtcact
    21421 atccctgctc atcaggtgaa tgctgaattc ttcgaagaag gcaaaatgtt tgacggctcc
    21481 tcgattggcg gctggaaagg cattaacgaa tccgacatgg tgctgatgcc agacgcatcc
    21541 accgcagtga ttgacccgtt cttcgccgac tccaccctga ttatccgttg cgacatcctt
    21601 gaacctggca ccctgcaagg ctatgaccgt gacccgcgct ctattgcgaa gcgcgccgaa
    21661 gattacctgc gttccactgg cattgccgac accgtactgt tcgggccaga acctgaattc
    21721 ttcctgttcg atgacatccg tttcggatca tctatctccg gttcccacgt tgctatcgac
    21781 gatatcgaag gcgcatggaa ctcctccacc caatacgaag gtggtaacaa aggtcaccgt
    21841 ccggcagtga aaggcggtta cttcccggtt ccgccggtag actctgctca ggatattcgt
    21901 tctgaaatgt gtctggtgat ggaacagatg ggtctggtgg ttgaagccca tcaccacgaa
    21961 gtagcgactg ctggtcagaa cgaagtggct acccgcttca ataccatgac caaaaaagct
    22021 gacgaaattc agatctacaa atatgttgtg cacaacgtag cgcaccgctt cggtaaaacc
    22081 gcgaccttta tgccaaaacc gatgttcggt gataacggct ccggtatgca ctgccacatg
    22141 tcgctgtcta aaaacggcgt taacctgttc gcaggcgaca aatacgcagg tctgtctgag
    22201 caggcgctgt actacattgg cggcgtaatc aaacacgcta aagcgattaa cgccctggca
    22261 aacccgacca ccaactctta taagcgtctg gtcccgggct acgaagctcc ggtaatgctg
    22321 gcttactctg cacgcaaccg ttctgcgtct atccgtattc cggtggtttc ttctccgaaa
    22381 gcacgtcgta tcgaagtacg tttcccggat ccggcggcta acccgtacct gtgctttgct
    22441 gccctgctga tggccggtct tgatggcatc aagaacaaga tccatccggg cgaagccatg
    22501 gacaaaaacc tgtatgacct gccgccggaa gaagcgaaag agatcccaca ggttgcaggc
    22561 tctctggaag aagcactgaa cgagctggat ctggaccgcg agttcctgaa agccggtggc
    22621 gtgttcacgg atgaagcgat cgatgcgtac atcgctctgc gtcgcgaaga agatgaccgc
    22681 gtgcgtatga ctccgcatcc ggtagagttt gagctgtact acagcgtcta atagttgaag
    22741 ttgtactacc cggcgcaaca acgccgggat ttagttgccg tggaaacttt cagcccatct
    22801 ctgcatgggc ttttttctcc gtcaattctc tgatgcttcg cgctttttat ccgtaaaaag
    22861 ctataatgca ctaaaatggt gcaacctgtt caggagactg ctttatggca acaggcacgc
    22921 agcccgatgc tgggcagatc ctcaactcgc tgattaacag tattttgtta atcgatgaca
    22981 acctggcgat ccattacgcc aaccccgccg cgcaacaact gctcgcccaa agctcccgca
    23041 aattgtttgg tacgccgtta ccggaactgt tgagctactt ctcattaaat attgagctga
    23101 tgcaagaaag tctggaggcg gggcaaggtt ttaccgataa cgaagtgacg ctggtcatcg
    23161 acggtcgctc gcatatcctt tctgtgacgg cccagcgtat gccggacggc atgatcctgc
    23221 tggagatggc gccgatggat aaccagcgcc gcttaagcca ggaacagcta caacatgctc
    23281 aacaagttgc tgcccgtgat ttagtgcgtg gcctggcaca tgagattaaa aatccgcttg
    23341 gcggtttacg tggcgcggcg cagctactca gcaaagcatt acctgacccg tcactgctgg
    23401 aatataccaa agtgattatc gaacaggcgg accggctgcg aaatctggtc gaccgtctgt
    23461 tggggccgca gctgcccggt acgcgcgtta ccgaaagtat tcacaaagta gctgaacgcg
    23521 tggtaacgct ggtgtcgatg gaactgccgg acaacgtgcg gttgattcgt gattacgatc
    23581 ccagcctgcc ggaactggcg cacgacccgg atcaaattga acaggtcttg ctgaatattg
    23641 tgcgcaatgc gctacaggcg ctggggccgg aaggcggtga aatcattctg cgtacccgca
    23701 ccgcgtttca actgacctta cacggcgaac gctatcggct ggcggcgcgg attgatgtgg
    23761 aagataacgg gccaggcatt ccgcctcatt tgcaggatac gctgttttac ccgatggtca
    23821 gcggccgcga aggtggcacc gggcttggct tatccatcgc tcgtaatttg attgatcagc
    23881 attcaggcaa aattgaattt accagttggc cagggcatac cgagttctcg gtttacctgc
    23941 ctatcaggaa ataaaggtga cgtttatgca acgagggata gtctgggtag tcgatgacga
    24001 tagttccatc cgttgggtgc ttgaacgtgc gctcgctgga gcgggtttaa cctgtacaac
    24061 atttgagaac ggcgcggagg tactggaggc gctggcgagc aaaacgccgg atgtgctgct
    24121 ttcagatatc cgtatgccgg gaatggacgg gctggcactg ctcaagcaga ttaaacagcg
    24181 ccatccgatg cttccggtca tcattatgac cgcacattcc gatctggatg ctgccgtcag
    24241 cgcctatcaa caaggggcgt ttgattatct gcccaaaccg tttgatatcg acgaagccgt
    24301 ggcgctggtt gagcgcgcca tcagtcatta ccaggaacag cagcagccgc gtaatgttca
    24361 gcttaacggc ccaacgaccg atatcatcgg cgaagcgcca gccatgcagg acgtgttccg
    24421 tattatcggt cggctttcgc gttcttctat tagcgtgctg attaacggcg aatccggcac
    24481 cggtaaagaa ctggtcgctc atgccctgca tcgccacagt ccgcgagcca aagcgccatt
    24541 tatcgctctg aatatggcgg ctatcccgaa ggatttgatc gaatcagaac tgtttggtca
    24601 cgagaaaggc gcgtttaccg gcgcgaatac cattcgtcag gggcgttttg aacaggctga
    24661 tggcggtaca ttatttctcg atgaaattgg cgatatgccg ctggatgtgc agacgcgttt
    24721 gctgcgcgtg ctggcagacg gtcagtttta ccgcgttggc ggctatgcgc cggtgaaagt
    24781 ggatgtgcgg attatcgctg ccactcacca gaatctcgaa cagcgggtgc aggaaggtaa
    24841 gttccgtgag gatctgttcc accgcctgaa cgttatccgc gttcatctgc cgccgctgcg
    24901 tgagcgtcgg gaagatattc cccgcctggc gcgccatttt ttacaggttg ccgcgcgaga
    24961 actgggcgta gaagcgaagt tgctgcatcc ggaaaccgaa gccgcgttga cgcgtctggc
    25021 gtggccaggc aacgtgcgcc agctggaaaa cacctgccgc tggctaacgg taatggctgc
    25081 cggacaggaa gtgttgattc aggatttgcc tggcgaactg tttgaatcaa cggttgcgga
    25141 gagtacttcg caaatgcaac cggacagttg ggcgacgctt cttgcgcagt gggcagacag
    25201 agcgctacgt tccggtcatc aaaatctgct ttccgaagcg cagccagagc tggagcggac
    25261 gttactgacg accgcgttgc gacatacgca ggggcataaa caggaggcgg cgcggctact
    25321 cggctggggc cgcaacaccc tgacgcgtaa gttaaaagag ctggggatgg agtgattcac
    25381 ggcttgtgtg taaagattga ttattgagcg caaattgctg gtattttgcg ctttactgtt
    25441 ccgataagtt cagtatgatc ttgcccggaa aacggggaga gtcattatgc tggaatcaat
    25501 aattaatctg gtatcgagtg gcgcagttga cagccacaca ccgcaaactg ctgttgctgc
    25561 cgtgctgtgt gccgcgatga ttgggctgtt tagttgagtt tcagattgct gatgaagtgc
    25621 tctttgttca tgccagatgc tgcgtgaacg ccttatccgg cctacaaaag caatattttc
    25681 aacaggtttt gtaggcctga taagcgtagc gcatcaggca atcttgttct cagcacattg
    25741 atggcgcttt cgttttctac tttgtaaacg aagcgccatt cactatttaa atcacccgag
    25801 agaactgctg catccgcgct ttctggcgca gataggtatc aaagcacatg caaatgttgc
    25861 ggatcagcaa gcgacctttc gccgtcacct gtattccctt ctcatccaca tccaccagcc
    25921 catcttttgc taacggggcg agcagcttga gatcttccgc aaagtaatca gcgaagtgca
    25981 aatcccactg tttctcaata ggggcgtaat ccagacggaa gttgcagatg agcgacttaa
    26041 tcacatcgcg gcgaatacag tcatcacgcg ttagcgcaat accacgccac agcgcattgc
    26101 cttgttcatc cacttgctga tagtactgct tcaactcttt ctggttctgc gcgtagcagt
    26161 cgccaatcat gctgatggcg gaaacgccca tccccagcag atcggtatcg ccctgagtgg
    26221 tgtagccctg gaaattacga tgcagcacgc cttcacgctg ggccaccgcc agctcgtcat
    26281 ccggacgggc aaagtgatcc ataccgataa actgatagcc cgattgcgtc aggaaggcga
    26341 tggtttcctg caggatatcg agtttttgct gcggactcgg caggtcagca tctttgattt
    26401 tgcgctgagc agcaaaaatg gtcggcagat gcgcatagtt aaagacgctc agacggtcag
    26461 ggtttagctc cgccacgcgt ttcagggtaa aggcgaaact ttccggcgtc tgtttcggca
    26521 ggccgtaaat caggtcgatg ttggtggagg taaagccaat ctcacgcgca tggttaagca
    26581 gcgcaaagat aaactcttca tcctgctcgc ggttaaccag acgttgcact tctttgttga
    26641 agtcctgcac gcccatactc aggcgattaa agccctcggc gcgtaaatga tcgagtacat
    26701 ccagttcgat ttcccgcgga tcgacttcga tcgaaatctc cgcatcggca ttgaactgga
    26761 agttttcgcg cagcagcttc atcaggcggc tgatttgcgc tttattaaga tacgtcggcg
    26821 ttccgccgcc ccagtgcagc tggctgacgt gacgcccggc gaacagcggt gcacgatgga
    26881 cgatttcttg ctccagcgcg tccagatact gatcggcctt gtgctgctgg cgagtaacaa
    26941 tcttattgca gccgcagaag taacaaagct tatggcagaa cgggatatgt acgtagagag
    27001 ataatggacg ctcaggatag cgcgccacgg cttgtaaaaa cgcctgttcg ccgaagtctt
    27061 ctgaaaactc cagcgcggtc gggtacgagg tgtatcgtgg cccggaatag ttatatttct
    27121 ggatcagggc cagatcccag tcgatttgct gtacagacat gctcactcct tccgatggcg
    27181 tctctggcgg ctacggcgta ccggcggatg cccgtttcgg gtcatcagcc ggttgcgcag
    27241 ccacttttgt cgccgcgaca accgctgtag tttaacgaat aaccacacca gataacatat
    27301 aaccggaagg gttatcagca gaacgggaag gcccacggcg gcaatccgtt agttgccccg
    27361 cagcagacgc atcatgtctt cttgcttctc gtcttcttcc tcttcttcgt catcgtcata
    27421 agagaggccg agtttctgca tcaactcatc aatacgatcc agtttagcat ccacccagga
    27481 ttgctcttcg gcactcaggg tttcgcctgc ttccagacgt tccagcagcg catccagacg
    27541 ctcatccgtt tctagtaatt ccaactccgc ctgcggtgaa agcataggtt tctcactctt
    27601 cggtttgtgc tgtttggtga ctttttcagt cacgcccaat ggaatagggg ttttactgcc
    27661 aatacgtgga tctttcggtg cgttctggcc tttgctgcct gacgtgttgt ttccgcccgc
    27721 tgcgcggctg cccggcgcat gaccacgacg ttttttctga cgcttgcggt cacgagcttc
    27781 ctgattcagc tcttcgcgcg ttttgcgacg cgcttttgca tgacctttgc tgcgtgatgg
    27841 tgaagatggt ttcataatgg ttcgtctttt agccgtttta ttcagtatag atttgcggcg
    27901 gaatctaaca gaaagcaagc aaagaaaaaa ggcgacagat tactctgtcg ccttttttcc
    27961 tgactcataa cccttaacgg gtcttacaat ccttgcaggc cgccaacaca ccctgttttt
    28021 ccattagccg gaacaatccg tgttgattcc atttccgttt aattacgtct ccggacgttt
    28081 gtcttcctga caatcctctg tcttcgcctc ctggcgctcc tgaccctcat cctgagtcat
    28141 tgttcctgtt agcgtcctcg ctctatgggt gctactttac gcctttgctt taaacaaaca
    28201 atcaaccgcc atcagtattt gcacattttc agagttttat ataaaagcaa ttaactgatt
    28261 tatatagctt atttgacatg catcgcagaa ttttctctgc tatttcgcat aaagactatc
    28321 ggcaatgtct cacaaagcgt atggctttta cttacaacgc tcacgccgaa aaatccgcct
    28381 tttattatgg ttcaggtata atcgccgcaa agctttgact gacggagacg accgctttga
    28441 ctaatttgaa ttatcaacag acgcattttg tgatgagtgc gcctgatatt cgccacctac
    28501 cttccgatac cggaattgaa gtggcttttg caggccgttc caacgcaggt aaatccagcg
    28561 cgctgaacac gctgactaac cagaaaagcc tggctcgtac ctcaaaaacc cctggacgca
    28621 cccagcttat caacctgttt gaagtggctg acggcaagcg tctggttgac ttgcctgggt
    28681 acggttatgc ggaagtcccg gaagagatga agcgcaaatg gcagcgcgcg ctcggcgagt
    28741 acctcgaaaa acgtcagagc ctacaaggtc tggtagtgct aatggatatt cgccatccgc
    28801 tgaaagattt ggatcagcag atgattgagt gggcggtaga cagcaatatc gccgtgctgg
    28861 tactgctgac caaagcggac aaactggcaa gcggcgcacg taaagcgcaa ttgaatatgg
    28921 tgcgtgaagc ggtgctggcg tttaacggtg atgtgcaggt tgaaacgttt tcttcgctga
    28981 agaaacaagg cgtggacaag ctgcggcaga aactggatac ctggtttaac gagatgcagc
    29041 ctgtagaaga aacgcaggac ggcgaataat tttcttacct taatgcttat gccggatgtg
    29101 gcgtatccgg cccataaatt caatagatta caattctacc gttggcctga taaacatggt
    29161 gcatcaggca ttacggatct ttcctttcgc ccaataaaaa acgccccagt cattactgac
    29221 tggggcggct aaaatattca gccaaatccg attacgtgaa gtaaaaggtc tgaaagatag
    29281 aacatcttac ctctgtaccc tacgcgacta actttactct tttttgttca gttcagaaag
    29341 cactttttgt aatttttttt cagttgttgc ataggaattt ctaatagcca tcacaaaacg
    29401 tgacagctta tgttgcttac ttacgaaaaa agggcatgtt caggcgaatc ttagtgcgcc
    29461 tgatcccagt tttcgccact ccccacttcc accagcaacg gcacatccag acgggtacag
    29521 ttttccatca gttgatgaat ctgcttcgca acggcatcga catcatctct atgaacttca
    29581 aataccagtt catcgtgtac ctgcatgatc atacgtacac gcggttgctc agcctgtaac
    29641 cacgcatcaa cggcaatcat cgcccgtttg ataatgtcgg cggcggttcc ctgcattggc
    29701 gcgttaatgg ctgcacgttc agccgctgca cgacgagcac cattgctgga tttgatatcc
    29761 ggcagataca gacggcgtcc gtccagcgtt tcaacgtagc cctgctcttt cgcctgagca
    29821 cgggtgcgtt ccatatactc cagcacgcca gggtagcgtt cgaagtaaag gtccatgtac
    29881 ttctgcgctt ctttacgtgg aatgttcaat tgccgcgcca gaccgaaagc actcatgcca
    29941 taaatcagac caaagttgat cgctttcgcg ctacggcgtt gctcgctggt gacggtttcc
    30001 agtggcaaac caaacacttc tgccgccgtt gcccggtgga tatcttttcc ttccgcgaat
    30061 gcggtcagca agcctttgtc acgcgagaga tgcgccataa tgcgcagttc aatctgcgag
    30121 tagtccgctg agacaatcac ataatcctct ggcgcaataa acgcctggcg gatacgacga
    30181 ccttcttcgt tacgcaccgg aatgttttgc aggttaggat cggttgacga taaacgtccc
    30241 gttgcagtta cagcctggtg ataagaggta tgcacacgcc cggttttcgg gttgatcatc
    30301 agcggcagct tgtcggtgta agtcgatttc agcttcgcca gaccacgata ctccagaatc
    30361 acttttggca acggatagtc cagcgccagt tcttccagta cctcttccga cgttgacggc
    30421 gcgccacccg gcgttttctt cagcggttta atgccctgtt tttcaaacag aatggtttgt
    30481 aactgcttgg tggaagaaag gttaaattcc tcacctgcaa tttcatgcgc tttcttttcc
    30541 agctcagcca gacgaagggt gagctcttca gaatgattgt gcagcacttt cggatcgatc
    30601 ttcacaccgt tacgttcaat gcgtgaaagt actggcacca gcggcatttc gatattctcg
    30661 aagacgttca acggcccttt gtgtttttgc agatccggcc acattttcag atgcaactgc
    30721 aaggtgacat cagcatcttc ggcggcgtaa cgtccggcct cttcgagggc aatctggtta
    30781 aaagtcagtt gatttttgcc tttacccgca atctcttcaa aagtgatggt tttgtgcttc
    30841 aaccaacgtt ccgcgaggct gtccatatcg tgacgcccgg caacgctatt gagaatgtag
    30901 gactccagca tggtatcaaa cgcaatccca cgcagctcaa tgccgtaatt cgccagaata
    30961 ccgcgatcgt atttcaggtt ttgcccgacc ttcagcgcct tttcatcttc cagcagcggt
    31021 tttagcaact cgagtgcacg ctcgcgagag atttgatcgg gcgcatcaag ataatcatga
    31081 gcaaccggaa tatatgccgc tacgcctggc tcgatagcaa aagaaagccc gaccaggtta
    31141 gctgagatgt tatcaaggct gtcggtttcg gtatcaaatg caaataccgg cgctttttcc
    31201 agcttcgcaa tccacgcttt cagtgtttct tcatcaagga tggtgacgta gttgtcataa
    31261 gaaatcaccg ttgccgtcac ttctggtgct tcgtctgcaa cactggtttc ctgtggcttc
    31321 gcggctggtt ttgccccttt ggcctgtaac catttgcccg cttcgacatc agcagtccag
    31381 cgtttgaact catacttttt gaacagcccc aacaactctt ccgctgccgg ttgctgcact
    31441 tccagttgtt cacaggtcag ctccagttca acgtcggttt taatcgtcgc cagctggtat
    31501 gagagataag caacttcttt gttttgctcg agcttcgctg ccattgtttt cgcgccacgg
    31561 aagctcaacc cagcaatttt ttctggctcg gcatacagcg tatccagccc gccaagacct
    31621 tgcagcaatg cctgcgcggt tttttcaccg acgcccggta cgccaggaat gttatcagag
    31681 gagtcaccca tcagcgccag gaaatcgatg atcagttctg gcggcacgcc gtacttattc
    31741 accacctctt ccggtccgag gatggtattc gtcatggtgt tgataagcgt aatatttggc
    31801 gtcaccagct gcgccatatc tttatcgcca gtgctgatca gcaccggacg cccggctttt
    31861 tcggcttcgc gcgccagagt accgataacg tcgtccgctt ctacgccaga aaccgccagc
    31921 agcggcagtc ccatcgcttt aaccatcgcg tgcaagggtt cgatttgtgc tcgcagatcg
    31981 tccggcattg gcgggcgatg tgatttgtaa tgttcaaaca gttcatcacg aaaggttttt
    32041 cccttggcgt caaagaccac cgctgcatgc gtcggtttat attgcatgat cagactgcgc
    32101 agcatgttga ggacaccata catcgcaccg gtcggctcgc ctgcgctgtt agtcagcggg
    32161 ggaaacgcgt gatatgcgcg ataaagataa gatgaaccat ctacaaggat aagtggattt
    32221 tgggggatct gaaccataat gtccgtgcct gtttatcaga ttatgggtaa aggatgccac
    32281 agacaggata aaaacatgag tttttcaccg cttttcgtga aaatcttttg tagatcttct
    32341 ggatcgctcg caaatcgtca tgtggataac tttgtgcata aattatttac atcgctttta
    32401 atttattatc atgcttttta cagcatgatg tttgtctttg tttttcatgc agttaaaaat
    32461 aaatccatcc ctaataattg agataatggg attttttcta atgtggatag atatgaatta
    32521 tttttctcct taaggatcat ccgttatttg ggtcgttttc tgctaaaatc tgcgccttgc
    32581 gcttctttag cgcactgtct atggctaacc ttttgaataa attcattatg acgagaatac
    32641 tcgctgcgat aacccttttg ctgagtatcg tattgaccat tttggtcact attttttgtt
    32701 ccgtcccgat catcattgcc gggattgtaa aactattgct acctgtgcca gtcatttggc
    32761 gaaaggtttc gcgtttttgt gattttatga tgtattgctg gtgtgaaggt ctggcggtat
    32821 tactgcatct gaacccacac ctgcaatggg aagttcacgg gctggaaggg ttaagtaaga
    32881 agaactggta tctgcttatt tgtaaccacc gtagttgggc agatattgtc gtactgtgcg
    32941 tgctgtttcg taagcacatt ccgatgaata aatatttcct caagcaacag ctggcctggg
    33001 tgccatttct tggcctggcg tgctgggcgc tggatatgcc gtttatgaag cgttattccc
    33061 gcgcttattt attacgtcat ccggagcgac gtggtaaaga tgttgaaacc actcggcgtt
    33121 cttgtgaaaa gtttcgcctg catcccacca ccattgtcaa tttcgttgaa ggctcccgtt
    33181 tcacgcaaga aaaacatcag caaacccact ccactttcca aaacttgttg ccaccaaagg
    33241 cggcaggcat tgcgatggcg ctaaatgtac tgggtaaaca attcgataaa ctgcttaatg
    33301 ttacgttgtg ttatccggac aataaccgtc agccgttctt cgatatgtta agcggtaaac
    33361 tgacgcggat tgtcgtacat gtggatttac agcctattgc cgatgagtta catggcgact
    33421 acatcaatga caaaagcttt aagcgccatt ttcagcaatg gctgaactca ttgtggcagg
    33481 aaaaagaccg acttctgacg tcgttgatgt catcacagcg gcaggacaag taaattttgc
    33541 tcgcctctga atgagcagag gcgagtgagt attttaatga acaaaacgtc cggcacgaga
    33601 cataaattct tcttcgctca tctcctgtcc gttaaaaaca actttacccg gtgtataacg
    33661 cagctgcagc gaggcggtat tgttgtcaat cgtgattaac tgaaacatct gacctaatgt
    33721 catcatcccg ctgatttgtt tatcagcctg cttttgcgct ttttccgcat ccattccttc
    33781 agataaatta agctgttttg cggtttctgt gacgacatta agcggtaact tcacatcaaa
    33841 attgagcgat ttgatatctt tgtttgtgga tgatgaagat ttggctgggt cggcaatact
    33901 gatatccaga ttggcattta gttcgccgag tgcgttcttc catcttaccg gttgtttaat
    33961 ggtcggctca ctttgttgta ataacggtaa atactctttg aacaatgcag cattcacttc
    34021 ttgcagagca acttcatcgt ttgctaattc agggtgtgca gcaagctgct tctgcatcgc
    34081 aatgttatac tggatgataa attggcgcac cgctgaggga tcaatagatt cagcaatcaa
    34141 tgaccactca ccactaccga gttgctgatt cccttttgtt aacttatcaa gttcgtaggt
    34201 cagattgaca ttgatataat ctttgtcttt aacgcgatcc agcctggttt ttgcaacgaa
    34261 agcatcgatt tgggcaacgt cttccccgtg attgataatg ttaatctgat taaatttcga
    34321 ctcgctttca ccaaccggaa atttcttttc ttctaatcgc gccagagaat caatggtaaa
    34381 actttttgcc gttactttgt tcattggtga taattgcaga acaatatctt cgactttgcc
    34441 ttcaatatcc aggttagaca atgaactact atcaccatta aaagtaaact ggccctcacc
    34501 aaaagaaaac tttccatact ctgcaggggc aacatctaat gtcgtagaga atgagccacc
    34561 aaacgcgaat cgcaatgtcg cttccacggg cgatttattt ttcgcggcga taaatagcgg
    34621 ttgtgtcagt tcattattca ctaagttcac ttttgccgct gccagtgctg ggatgacatt
    34681 accatgcatt aacattgtga tgggcaacgg accgtgctca acatccacat caaatacaac
    34741 tttctggcct ggcttgatat tgagatcggg tgcaccgtta tcgaatgtca tttgcatttg
    34801 aaaacgggaa ttaaaaaagc ctttgtcaaa attttgataa cttaacgtca tatcgtaggc
    34861 atgttcacct ttctttttag catcgttaaa atctttaata aatttttcga caccaggctg
    34921 aatttgcgta cctgtgtacc atgtgccacc accccagatt acggctaacg caacaataac
    34981 acctgtagct gacttacgta tcatataaat attccctgat aaaacagcaa aactttgaat
    35041 atccacttat gctgaaataa tgtccag
//
LOCUS       DAZKIP010000038        34664 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZKIP010000038 DAZKIP010000000
VERSION     DAZKIP010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.038"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            1..668
                     /locus_tag="ACHOUK_002092"
                     /pseudo
     CDS             1..668
                     /locus_tag="ACHOUK_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228226.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="toxin"
     gene            complement(818..2182)
                     /gene="mnmE"
                     /locus_tag="ACHOUK_002093"
     CDS             complement(818..2182)
                     /gene="mnmE"
                     /locus_tag="ACHOUK_002093"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFP9897548.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSAKTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            complement(2288..3934)
                     /gene="yidC"
                     /locus_tag="ACHOUK_002094"
     CDS             complement(2288..3934)
                     /gene="yidC"
                     /locus_tag="ACHOUK_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFP9897549.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            complement(3937..4194)
                     /gene="yidD"
                     /locus_tag="ACHOUK_002095"
     CDS             complement(3937..4194)
                     /gene="yidD"
                     /locus_tag="ACHOUK_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFP9897550.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFNTREH"
     gene            complement(4158..4517)
                     /gene="rnpA"
                     /locus_tag="ACHOUK_002096"
     CDS             complement(4158..4517)
                     /gene="rnpA"
                     /locus_tag="ACHOUK_002096"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFP9897551.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVANLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            complement(4534..4674)
                     /gene="rpmH"
                     /locus_tag="ACHOUK_002097"
     CDS             complement(4534..4674)
                     /gene="rpmH"
                     /locus_tag="ACHOUK_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFP9897552.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            complement(4904..4984)
                     /locus_tag="ACHOUK_002098"
     CDS             complement(4904..4984)
                     /locus_tag="ACHOUK_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9897553.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            5281..6684
                     /gene="dnaA"
                     /locus_tag="ACHOUK_002099"
     CDS             5281..6684
                     /gene="dnaA"
                     /locus_tag="ACHOUK_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFP9897554.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKSVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            6689..7789
                     /gene="dnaN"
                     /locus_tag="ACHOUK_002100"
     CDS             6689..7789
                     /gene="dnaN"
                     /locus_tag="ACHOUK_002100"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFP9897555.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            7789..8862
                     /gene="recF"
                     /locus_tag="ACHOUK_002101"
     CDS             7789..8862
                     /gene="recF"
                     /locus_tag="ACHOUK_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFP9897556.1"
                     /translation="MSLTRLLIRDFRNIETADLALCPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            8891..11305
                     /gene="gyrB"
                     /locus_tag="ACHOUK_002102"
     CDS             8891..11305
                     /gene="gyrB"
                     /locus_tag="ACHOUK_002102"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFP9897557.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(11329..11427)
                     /locus_tag="ACHOUK_002103"
                     /pseudo
     CDS             complement(11329..11427)
                     /locus_tag="ACHOUK_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11545..11943
                     /gene="yidB"
                     /locus_tag="ACHOUK_002104"
     CDS             11545..11943
                     /gene="yidB"
                     /locus_tag="ACHOUK_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFP9897558.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQPVSGEQLESALGTNAVSDLGQKLGVDTSTASCLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            12058..12870
                     /gene="yidA"
                     /locus_tag="ACHOUK_002105"
     CDS             12058..12870
                     /gene="yidA"
                     /locus_tag="ACHOUK_002105"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFP9897559.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGNTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(12916..13572)
                     /gene="yidX"
                     /locus_tag="ACHOUK_002106"
     CDS             complement(12916..13572)
                     /gene="yidX"
                     /locus_tag="ACHOUK_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HFP9897560.1"
                     /translation="MKLNFKGFFKAASLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVANDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            13708..13821
                     /locus_tag="ACHOUK_002107"
                     /pseudo
     CDS             13708..13821
                     /locus_tag="ACHOUK_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13818..14882)
                     /gene="cbrA"
                     /locus_tag="ACHOUK_002108"
     CDS             complement(13818..14882)
                     /gene="cbrA"
                     /locus_tag="ACHOUK_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFP9897561.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRYLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRSVLLKQPEKINAAYWHATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGMAHIPQLKD"
     gene            14983..16197
                     /gene="yidR"
                     /locus_tag="ACHOUK_002109"
     CDS             14983..16197
                     /gene="yidR"
                     /locus_tag="ACHOUK_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFP9897562.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRVSQGAHVGVVTVHPKSEKYVFIHGPENPHETWHYDFHHRRGVIVE
                     GGKMNNLDAMDITVPYTSGALRGGSHVHVFSPNGEMVSFTYNDHVMHQFDSALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGIVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLGNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            complement(16199..16531)
                     /gene="yidQ"
                     /locus_tag="ACHOUK_002110"
     CDS             complement(16199..16531)
                     /gene="yidQ"
                     /locus_tag="ACHOUK_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFP9897563.1"
                     /translation="MIRNVLLAFMICSGMALLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     misc_feature    16771..16843
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            16837..17250
                     /gene="ibpA"
                     /locus_tag="ACHOUK_002111"
     CDS             16837..17250
                     /gene="ibpA"
                     /locus_tag="ACHOUK_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFP9897564.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            17362..17790
                     /gene="ibpB"
                     /locus_tag="ACHOUK_002112"
     CDS             17362..17790
                     /gene="ibpB"
                     /locus_tag="ACHOUK_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFP9897565.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            17986..19647
                     /gene="yidE"
                     /locus_tag="ACHOUK_002113"
     CDS             17986..19647
                     /gene="yidE"
                     /locus_tag="ACHOUK_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFP9897566.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(19644..20360)
                     /gene="yidP"
                     /locus_tag="ACHOUK_002114"
     CDS             complement(19644..20360)
                     /gene="yidP"
                     /locus_tag="ACHOUK_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9897567.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPDKS"
     gene            20656..22272
                     /locus_tag="ACHOUK_002115"
     CDS             20656..22272
                     /locus_tag="ACHOUK_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9897568.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            22272..23594
                     /locus_tag="ACHOUK_002116"
     CDS             22272..23594
                     /locus_tag="ACHOUK_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HFP9897569.1"
                     /translation="MTKFSVVVAGGGSTFTPGIVLMLLANQDRFPLRALKFYDNDGSR
                     QEVIAEACKVILKEKAPDIAFSYTTDPEVAFSDVDFVMAHIRVGKYPMRELDEKIPLR
                     HGVVGQETCGPGGIAYGMRSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLR
                     PNAKILNICDMPIGIESRMAQIVGLQDRKQMRVRYYGLNHFGWWTSIEDLQGNDLMHQ
                     LRQYVSKHGYVPPQQDTHTEASWNDTYAKARDVQALDPDTLPNTYLKYYLFPDYVVQH
                     SNPEHTRANEVMEHREKQVFDACRAITAAGNSAAGKLEIDEHASYIVDLAAAIAFNTQ
                     ERMLLIVPNNGAIHNFDDEAMVEIPCLVGHNGPEPLVVGDIPQFQKGLMSQQVAVEKL
                     VVDAWEQRSYQHLWQAITLSKTVPSASVAKAILDELLEANKAYWPELR"
     gene            complement(23591..24484)
                     /gene="yidL"
                     /locus_tag="ACHOUK_002117"
     CDS             complement(23591..24484)
                     /gene="yidL"
                     /locus_tag="ACHOUK_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP9897570.1"
                     /translation="MNGKLQTSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPIGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            24651..26366
                     /gene="yidK"
                     /locus_tag="ACHOUK_002118"
     CDS             24651..26366
                     /gene="yidK"
                     /locus_tag="ACHOUK_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFP9897571.1"
                     /translation="MNSLQILSFVGFTLLVAIITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRMIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFLRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            26363..27856
                     /gene="yidJ"
                     /locus_tag="ACHOUK_002119"
     CDS             26363..27856
                     /gene="yidJ"
                     /locus_tag="ACHOUK_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFP9897572.1"
                     /translation="MKRPNFLFIMTDTQAPNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYELGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALKPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDAHFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDAQPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(27903..28352)
                     /gene="yidI"
                     /locus_tag="ACHOUK_002120"
     CDS             complement(27903..28352)
                     /gene="yidI"
                     /locus_tag="ACHOUK_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFP9897573.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVERKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGARVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            28461..28808
                     /gene="yidH"
                     /locus_tag="ACHOUK_002121"
     CDS             28461..28808
                     /gene="yidH"
                     /locus_tag="ACHOUK_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFP9897574.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            28798..29160
                     /gene="yidG"
                     /locus_tag="ACHOUK_002122"
     CDS             28798..29160
                     /gene="yidG"
                     /locus_tag="ACHOUK_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFP9897575.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            29157..29654
                     /gene="yidF"
                     /locus_tag="ACHOUK_002123"
     CDS             29157..29654
                     /gene="yidF"
                     /locus_tag="ACHOUK_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFP9897576.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDMTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRGCQACDWHLLCPHAREETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(29662..30846)
                     /gene="emrD"
                     /locus_tag="ACHOUK_002124"
     CDS             complement(29662..30846)
                     /gene="emrD"
                     /locus_tag="ACHOUK_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFP9897577.1"
                     /translation="MKRHRNINLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            30928..31002
                     /gene="ysdE"
                     /locus_tag="ACHOUK_002125"
     CDS             30928..31002
                     /gene="ysdE"
                     /locus_tag="ACHOUK_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFP9897578.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            complement(31126..31215)
                     /gene="tisB"
                     /locus_tag="ACHOUK_002126"
     CDS             complement(31126..31215)
                     /gene="tisB"
                     /locus_tag="ACHOUK_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFP9897579.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            31780..31878
                     /gene="ivbL"
                     /locus_tag="ACHOUK_002127"
     CDS             31780..31878
                     /gene="ivbL"
                     /locus_tag="ACHOUK_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFP9897580.1"
                     /translation="MATSMLNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            31984..33672
                     /gene="ilvB"
                     /locus_tag="ACHOUK_002128"
     CDS             31984..33672
                     /gene="ilvB"
                     /locus_tag="ACHOUK_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFP9897581.1"
                     /translation="MASSGTSSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYKQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            33676..33966
                     /gene="ilvN"
                     /locus_tag="ACHOUK_002129"
     CDS             33676..33966
                     /gene="ilvN"
                     /locus_tag="ACHOUK_002129"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFP9897582.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            34229..>34664
                     /locus_tag="ACHOUK_002130"
     CDS             34229..>34664
                     /locus_tag="ACHOUK_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9897583.1"
                     /translation="MASPINSGIMMANLCPSTFLDKNRNVAAELDIKNNEKKYSPGSN
                     FAKWMLQEIKRLILNIMSGSRSINTDILDYFHPMPGTENNGNRTWVAATGEDEYIEIK
                     QTGDKSFNITLVGRDKPSRKEIPYSGVAVATIIKSLSEKTSAL"
BASE COUNT         8390 a         8748 c         9153 g         8373 t
ORIGIN      
        1 tatattgcta gccaatataa aagccaaaat gtttaaaaat gaattaaata aagaagatat
       61 attgaaactg atggcagcaa gagaaaaagt ggcgggagca tcagacaaat ggacgaaggc
      121 atcaggctta tattctgcga tagtgaaagg gcatacgaag attgttgctg cctggatgga
      181 gacagctgaa gtgatagcca gccattatga aaatgataaa gatgtagtga gagaactcct
      241 gtcgctgagc agaaataatg cagtttgctc tttgtatgtt gccagctata agacaatgag
      301 taagcaggtc attgatgtat atctgaatgc ggcgattcgc ctggcgttgc aacacgggtt
      361 cactttcgat gagattttgg agcagtttac ccgtgacttt gatgggaagt cattctctct
      421 tgcggtagag aaagcggatg atatatatgg gtctctggct gaaaatattc aaaattgtgg
      481 ttggtgaaaa tgaaaattat cttaaagatg tgatgatgca actggaagcg aagaataatg
      541 aaggcaaata tgtaataagc aaggcaaatg gcaatcctgt atttaaagaa ctattctgga
      601 aagcaataga cgaatttaac tttccgcaag aggagcttaa tcggctaaag caatatcgtt
      661 ctctgtaaag acaggactgc aagatgttga aagtcgctcc agccagtagt aaaggcagtt
      721 ttggtgcagg atgctcataa aaaaacaagc gctgttccgt tctgatagtt agtttccccc
      781 agaacggaac agtgcccttt atatcaattt agacgactta cttaccaata cagaagctgg
      841 agaaaatccg ccccagcaaa tcatcagagg taaattcccc ggtgatttcg cttaagttct
      901 gctgtgccag gcgcaactct tccgccagca gttcgcccgc ccaggcaccc aacagttgcg
      961 ctttaccttg ctgcaaatgc tccgctgcct gttccagcgc ctgaaggtgg cgacgacgcg
     1021 ccaggaagcc gccttccatg ttggtgtcaa agcccatgct ctgtttgaga tggttacgca
     1081 gcacgtccac gccttcgcca gtctttgccg agagacgaat caacgcatga ccgttcactt
     1141 cactcattcc cagcgtttcg ccagtgatat cggctttatt gcgtaccacg gtgatcggca
     1201 gtttcgcggg cagacgggcg ataaattccg gccagatctc cgccggatca acggcgtctg
     1261 tcgtggtgcc atcgaccata aacagcacgc ggtcggcttg ttcaatttct tgccacgcgc
     1321 gctcgatacc aatacgttcc acttcatcgc tggcttcacg tagcccggcg gtgtcgatga
     1381 tatgcagcgg cattccgtca atgtggatat gctcacgcaa cacgtcacgc gtggttccgg
     1441 cgatatcggt tacgattgct gcttcacggc cagccagcgc gtttaacagg ctcgatttac
     1501 cggcattagg acgtccggca atcaccacct tcatcccttc gcgcaacaaa ctaccctgac
     1561 gtgcttcagc acgcactgca tcgagatcgg caatcacgtc attaagctgg gcttcaattt
     1621 ttccgtcgga gaggaaatcg atctcttcat cggggaaatc aattgccgct tcgacgtaaa
     1681 tgcgcaagtg ggtgagggct tccaccagat ggttgacccg tgcggagaat gcgccttgca
     1741 gcgagttaag tgccgaacgg gctgcctgtt ccgaactggc atcgataaga tcggcaatcg
     1801 cctcggcctg ggctaagtcg agtttatcgt taagaaacgc gcgttcggaa aactcaccag
     1861 ggcgagcaat ccgcaggccg ggaatggtca gaatgcgttt taacagcagg tcgagaatca
     1921 ccggaccgcc atgaccctgc agttccagca catcttcgcc ggtgaacgag ttcgggccag
     1981 ggaaccatag cgcaatcccc tgatcgagca cgctgccatc ggcgtcttta aacggaagat
     2041 aatcggcgta gcgcggctta ggcagtttac ccagcacggt ttcggcaact tcacgggctt
     2101 tgaagccgga gatgcgcagg atgccaacgc cgccacgtcc cggaggcgtg gcctgggcta
     2161 cgatagtgtc attatcgctc atgatgttcc tgttgctttg tgtggcggat acgcggtgct
     2221 tatccgccct acgaaaagaa aaaaggcggt caactgaccg cccttatttt agcgaaaact
     2281 caccgaatca ggattttttc ttctcgcggc tatgcaggcc acgtttttcc agaccacggt
     2341 aaatcagctg ctgctgaata atggttacca ggttgctgac gatatagtac agcaccagac
     2401 ctgacgggaa ccacaggaag aacacggtga agatgaccgg cataaaggtc atgatcttct
     2461 gctgcatcgg gtcggtcaca gtggtcggcg acatcttctg aatgaagaac atcgttacgc
     2521 ccatcaggat cggcaggatg tagtacgggt cctgtgcaga caggtcgtgg atccacagtg
     2581 caaacggtgc ctgacgcagt tcaacggaac ccatcagcat gtagtacaac gccaggaaga
     2641 ttggcatctg gatcagcagc gggaagcagc cgcccagcgg gttaaccttc tcagctttgt
     2701 acagcgccat catttcctgg ctgatacgct gtttgtcatc gcccagacgc tcacgcattg
     2761 cctgaatctt cggctgcagc atacgcatct tcgccatgga ggtgtactgc gctttggtca
     2821 gcgggtacat gatgccacga acgataaagg tgatgatgat aatggagaag ccccagttac
     2881 ccacaaagct atggatccat ttcagcagtt tgaacagcgg ctgagagatg aaccacaacc
     2941 aaccgtaatc aacggtcaga tccaggtgcg gagcaacagc tgccattttg tcctggattt
     3001 ccgggccaac ccacagggtg ctgttcatcg cgccagtctg accaggctga accagtaccg
     3061 gctgagattt atagccgata gcggcgatgc cgttacccag attagcagta tagaagttgt
     3121 tggtaccgtc gttatgcggg atccacgccg tcgcgaaata ctgttgcagc attgccaccc
     3181 aaccaccttt cgaagagatg ttcaggtttt cgttatcggc aatggtatcg aacttgtatt
     3241 tctcgtactt ctcgtcaggc gtggagtacg ccgcgccacg gaaggtgtgc agtgcgaagt
     3301 tgctgcttcc ggtatcgaga tgcggtggca gagtgatgga ttgcttcaac tgaccaaagg
     3361 tggagatttc cagcggtttc tcgccagcgt tctgcacgtt gtagttgacg ttgacagcgt
     3421 aatcaccccg tttcaggaca aacgttttgg taaacgtgtt gcctgccgcg tcggtatacg
     3481 tcatcggcac ctgcagttcg ttttgacctt cagccagcac ataagcgtct ttttcaacgt
     3541 tatacagcgg acgcgggccg ttagccgggt tatctgggcc atcacgaccg gtcagaccgc
     3601 tctgtgcctg ataaataaac tgcggtgaag tttccagcag ctggaacggc tgggtagagt
     3661 tcagctcttt cgggtaagca ggcagcagag cttgctcaac atcaccacca cgggtgttga
     3721 tggtcagatc aagcacgtcg gtcttaaccg agatcagttt cccctggcca ctggccggta
     3781 cgccctggtc ggcggcgcta cccgctgcgg tggtcgttgt ctgcgtggtc tgttgggcct
     3841 gaggttgcgg gtttttatcc tgctcccagg cttgccagat catgaaagac acgaacagca
     3901 aagcgatgac taaaagattg cgttgcgaat ccatcgttag tgttctctgg tattaaatgg
     3961 tccgggcggg acgggatcgt caccaccagg gtgtaaaggg tggcatttta atacgcgttt
     4021 caccgtcaac caactgcctt ttatcactcc aaacctgcgc aatgcctcaa ttccgtagct
     4081 tgaacaggtt ggagtgaaac gacaatgcgg cccgagtagc ggactaatca ggcgttgata
     4141 gacccgaatg agggctatca ggacccgcga gccaggcgac agtggcggcg ccataatttt
     4201 tccaacgctt ccgagagagc acggttatcg aggttggcaa cccctttttt cgccaccacc
     4261 acgaaatcca tagccgggag ttcatgttgg cgcagacgga agctttcacg cgtcagacgt
     4321 ttaatccgat tgcgttcatg ggcgcgtcga acgtttttct tggcgactgt aagaccgata
     4381 cggggatgcc ccagcgaatt caggcggccg agaatggtaa tttgcggcgt gccagcccgt
     4441 tgtggctgct ggaagacgaa tgtgaattga ctgggagtta acaagcgtaa ctccctggga
     4501 aatgcgagct taaccactca ggggttagct ttattactta gaaacggtca gacgagcgcg
     4561 gcctttagca cgacgacgtg ccagaacctg acgaccattt ttagtagcca tacgagcacg
     4621 gaagccgtga gaacggttgc gcttcagtac agacggttga aaagtgcgtt tcatggcgat
     4681 ttctacctaa acttgaataa attcaatggc tttattggat atccgccgaa aaatgaaacg
     4741 atggacaccg aagccatggg tgattaaaga ggccggattg taataattgt acactccgga
     4801 gtcaattctc tttccttatt taccgcgctt ttccgcacct tttcgcaggg aaaacgtacg
     4861 gcctcacacc agtgaaaacc agcatggcgc gccgggtgga ggattatacg ggctgatggg
     4921 taaagcgcaa ggatcgtcct ggatctttat tagatcgatt aagccaattt ttgtctatgg
     4981 tcattaaatt ttccaatatg cggcgtaaat cgtgcccgcc tcgcggcagg atcgtttaca
     5041 cttagcgagt tctggatcat cctgtggata aatcgggaaa atctgtgaga aacagaagat
     5101 ctcttgcgca gtttaggcta tgatccgcgg tcccgatcgt tttgcaggat cttgatcggg
     5161 catataaccg cagacagcgg ttcgtgcgtc accctcaagc agggtctttt cgacgtacgt
     5221 caacaatcat gaatgtttca gccttagtca ttatcgactt ttgttcgagt ggagtccgcc
     5281 gtgtcacttt cgctttggca gcagtgtctt gcccgattgc aggatgagtt accagccaca
     5341 gaattcagta tgtggatacg cccattgcag gcggaactga gcgataacac gctggccctg
     5401 tacgcgccaa accgttttgt cctcgattgg gtacgggaca agtaccttaa taatatcaat
     5461 ggactgctaa ccagtttctg cggagcggat gccccacagc tgcgttttga agtcggcacc
     5521 aaatcggtga cgcaaacgcc acaagcggca gtgacgagca acgtcgcggc ccctgcacag
     5581 gtggcgcaaa cgcagccgca acgtgctgcg ccttctacgc gctcaggttg ggataacgtc
     5641 ccggccccgg cagaaccgac ctatcgttct aacgtaaacg tcaaacacac gtttgataac
     5701 ttcgttgaag gtaaatctaa ccaactggcg cgcgcggcgg ctcgccaggt ggcagataac
     5761 cctggcggtg cctataaccc gttgttcctt tatggcggca cgggtctggg taaaactcac
     5821 ctgctgcatg cggtgggtaa cggcattatg gcgcgcaagc cgaatgccaa agtggtttat
     5881 atgcactccg agcgctttgt tcaggacatg gttaaagccc tgcaaaacaa cgcgatcgaa
     5941 gagtttaaac gctactaccg ttccgtagat gcactgctga tcgacgatat tcagtttttt
     6001 gctaataaag aacgatctca ggaagagttt ttccacacct tcaacgccct gctggaaggt
     6061 aatcaacaga tcattctcac ctcggatcgc tatccgaaag agatcaacgg cgttgaggat
     6121 cgtttgaaat cccgcttcgg ctgggggctg actgtggcga tcgaaccgcc agagttagaa
     6181 acccgcgtgg cgatcctgat gaaaaaggcc gacgaaaatg acattcgttt gccaggcgaa
     6241 gtggcgttct ttatcgccaa gcgtctacga tctaacgtac gtgagctgga aggcgcgctg
     6301 aaccgcgtca ttgccaacgc caactttacc ggacgggcga tcactatcga cttcgtgcgt
     6361 gaggcgctgc gcgacttgct ggcattgcag gaaaaactgg tcaccatcga caatattcag
     6421 aagacggtgg cggagtacta caagatcaaa gtcgcggatc tcctttccaa gcgtcgatcc
     6481 cgctcggtgg ctcgtccgcg tcagatggcg atggcgctgg cgaaagagct gactaaccac
     6541 agtctgccag agattggcga tgcgtttggt ggtcgtgacc acactacggt gcttcatgcc
     6601 tgccggaaga tcgagcagct gcgtgaagag agccacgata tcaaagaaga tttttcaaat
     6661 ttaatcagaa cattgtcatc gtaaacctat gaaatttacc gtagaacgtg agcatttatt
     6721 aaaaccgcta caacaggtga gcggtccgtt aggtggtcgt cctacgctac cgattctcgg
     6781 taatctgctg ttacaggttg ctgacggtac gttgtcgctg accggtactg atctcgagat
     6841 ggaaatggtg gcacgcgttg cgctggttca gccacacgag ccaggagcga cgaccgttcc
     6901 ggcgcgcaaa ttctttgata tctgccgtgg tctgcctgaa ggcgcggaaa ttgccgtgca
     6961 gctggaaggt gagcggatgc tggtgcgctc cgggcgtagc cgtttttcgc tgtctaccct
     7021 gccagcggcg gatttcccga acctcgatga ctggcagagt gaagtcgaat ttaccctgcc
     7081 gcaggcgacg atgaagcgtc tgattgaagc gacccagttt tctatggcgc atcaggacgt
     7141 tcgctattac ttaaacggta tgctgtttga aaccgaaggt gaagaactgc gcaccgtagc
     7201 gaccgacggc caccgtctgg cggtctgttc aatgccaatt ggtcaatctt tgccaagcca
     7261 ttcggtgatc gtgccgcgta aaggcgtgat tgaactgatg cgtatgctcg acggcggcga
     7321 taatccgctg cgtgtgcaga ttggcagcaa caatattcgc gcccacgttg gcgactttat
     7381 cttcacctct aaactggtgg atggtcgctt cccggattac cgccgcgttc tgccgaagaa
     7441 tccggacaaa catctggaag caggctgcga tctcctcaag caggcgtttg ctcgcgcggc
     7501 aattctctct aacgagaaat tccgcggtgt gcgcctgtat gtcagcgaaa accagctgaa
     7561 aatcaccgcc aacaacccgg aacaggaaga agcggaagag atcctcgacg ttacctatag
     7621 cggtgcggag atggaaatcg gcttcaacgt cagctatgtg ctggatgttc tgaacgcgct
     7681 gaaatgcgaa aacgtccgca tgatgctgac cgattcggtt tccagcgtgc agattgaaga
     7741 tgcagccagc cagagcgcgg cttatgttgt catgccaatg agactgtaat gtccctcacc
     7801 cgcttgttga tccgcgattt ccgcaacatt gaaaccgcgg atctcgcttt atgtcccggc
     7861 tttaactttc tggtaggtgc caacggcagt ggcaaaacca gcgtgctgga agccatctat
     7921 acgctcggcc atggtcgggc gtttcgcagt ttgcagattg gtcgcgtcat tcgccacgag
     7981 caggaggcgt ttgttctcca cgggcgatta cagggcgaag agcgcgagac ggcgattggc
     8041 ttaaccaaag acaaacaggg cgacagcaaa gtccgcatcg acggtactga cgggcataaa
     8101 gtcgcggaac tggcgcacct gatgccaatg cagctgataa cgccagaagg gtttacttta
     8161 ctcaacggcg gccccaaata cagaagagca ttcctcgact ggggatgctt tcacaacgaa
     8221 cccggatttt tcaccgcctg gagcaatctc aagcgattgc tcaagcagcg caatgcggcg
     8281 ctgcgccagg tgacacgtta cgaacagcta cgcccgtggg ataaagaact gatcccgctg
     8341 gcggagcaaa tcagtacctg gcgcgcggag tatagcgccg gtatcgcggc cgatatggct
     8401 gatacctgta agcaatttct ccctgagttt tctctgactt tctctttcca gcgcggctgg
     8461 gagaaagaga cagaatatgc tgaggtgctg gaacgtaatt ttgaacgcga tcgccagcta
     8521 acctacaccg cgcatggccc gcataaagcg gacttacgca ttcgcgccga cggtgcgccg
     8581 gtggaagata ccttatcgcg tgggcagctt aagctgttga tgtgcgcctt acgtctggcg
     8641 caaggagagt tcctcacccg tgaaagcggg cggcggtgtc tctacctgat agatgatttt
     8701 gcctctgagc ttgatgatga gcgtcgcggg ctgcttgcca gccgcttaaa agctacgcaa
     8761 tcgcaggtct ttgtcagcgc gatcagtgct gaacacgtta tagacatgtc ggacgaaaat
     8821 tcgaagatgt ttaccgtgga aaagggtaaa ataacggatt aacccaagta taaatgagcg
     8881 agaaacgttg atgtcgaatt cttatgactc ctccagtatc aaagtcctga aagggctgga
     8941 tgcggtgcgt aagcgcccgg gtatgtatat cggcgacacg gatgacggca ccggtctgca
     9001 ccacatggta ttcgaggtgg tagataacgc tatcgacgaa gcgctcgcgg gtcactgtaa
     9061 agaaattatc gtcaccattc acgccgataa ctctgtctct gtacaggatg acgggcgcgg
     9121 cattccgacc ggtattcacc cggaagaggg cgtatcggcg gcggaagtga tcatgaccgt
     9181 tctgcacgca ggcggtaaat ttgacgataa ctcctataaa gtgtccggcg gtctgcacgg
     9241 cgttggtgtt tcggtagtaa acgccctgtc gcaaaaactg gagctggtta ttcagcgcga
     9301 gggtaaaatt caccgtcaga tctacgaaca cggtgtaccg caggcaccgc tggcggttac
     9361 cggcgagact gaaaaaaccg gcaccatggt gcgtttctgg cccagcctcg aaaccttcac
     9421 caatgtgacc gagttcgaat atgaaattct ggcgaaacgt ctgcgtgagt tgtcgttcct
     9481 caactccggc gtttccattc gtctgcgcga caagcgtgac ggcaaagaag accacttcca
     9541 ctatgaaggc ggcatcaaag cgttcgttga atatctgaac aagaacaaaa cgccgatcca
     9601 cccaaatatc ttctacttct ccaccgaaaa agacggtatc ggcgttgaag tggcgctgca
     9661 gtggaacgat ggcttccagg aaaacatcta ctgctttacc aacaacattc cgcagcgtga
     9721 cggcggtact cacctggcag gcttccgtgc ggcgatgacc cgtaccctga atgcctacat
     9781 ggacaaagaa ggctacagca aaaaagccaa agtcagcgcc accggtgacg atgcgcgtga
     9841 aggcctgatt gcggtcgttt ccgtaaaagt gccggacccg aaattctcct cccagaccaa
     9901 agacaaactg gtttcttctg aggtgaaatc ggcggttgaa cagcagatga acgaactgtt
     9961 ggcggaatac ctgctggaaa acccaactga cgcgaaaatc gtggttggca aaattatcga
    10021 tgctgcccgt gcccgtgaag cggcgcgtcg cgcgcgtgaa atgacccgcc gtaaaggtgc
    10081 gctcgactta gcgggcctgc cgggcaaact ggcagactgc caggaacgcg atccggcgct
    10141 ttccgaactg taccttgtgg aaggggactc cgcgggcggc tctgcgaagc aggggcgtaa
    10201 ccgcaagaac caggcgattc tgccgctgaa gggtaaaatc ctcaacgtcg agaaagcgcg
    10261 cttcgataag atgctctctt ctcaggaagt ggcgacgctt atcaccgcgc ttggttgtgg
    10321 tatcggtcgt gacgagtaca acccggacaa actgcgttat cacagcatca tcatcatgac
    10381 cgatgcggac gtcgacggct cgcacattcg tacgctgctg ttgaccttct tctatcgtca
    10441 gatgccggaa attgttgaac gcggtcacgt ctacatcgct cagccgccgc tgtacaaagt
    10501 gaagaaaggc aagcaggaac agtacattaa agacgacgaa gcgatggatc agtaccagat
    10561 ctctatcgcg ctggacggcg caacgctgca caccaacgcc agcgcaccgg cattggctgg
    10621 cgaagcgtta gagaaactgg tgtctgagta caacgcgacg cagaaaatga tcaaccgcat
    10681 ggagcgtcgt tatccgaaag caatgctgaa agagcttatc tatcagccga cgctgacgga
    10741 agccgacctc tctgatgagc agaccgttac ccggtgggtg aacgcgctgg tcagcgaact
    10801 gaacgacaaa gaacagcacg gcagccagtg gaagtttgat gtccacacca atgccgaaca
    10861 aaacctgttc gagccgattg ttcgcgtgcg tacccacggt gtggatactg actatccgct
    10921 ggatcacgag tttatcactg gtggcgaata tcgtcgtatc tgcacgctgg gtgagaaact
    10981 gcgtggcttg ctggaagaag atgcgtttat cgaacgtggc gagcgtcgtc agccggtagc
    11041 cagcttcgag caggcgctgg actggctggt gaaagagtcc cgtcgcggcc tctccatcca
    11101 gcgttataaa ggtctgggcg agatgaaccc ggaacagctg tgggaaacca ccatggaccc
    11161 ggaaagccgt cgtatgctgc gcgttaccgt taaggatgcg attgccgctg accagttgtt
    11221 caccacgctg atgggcgacg ccgttgaacc gcgccgtgcg tttattgaag agaacgccct
    11281 gaaagcggcg aatattgata tttaatggcg ttaacaatgc gagcgtgcct gatgcgctac
    11341 gcttatcagg cctacgagac tcctgcaata tattgaattt gcatgttttt gtaggccgga
    11401 taaggcgttc acgccgcatc cggcaagaag ctataagaaa agggcggaga tcatctccgc
    11461 cctttttatt tctgcaatcc gcaattatca tcccgcagtt ctatactttt tgggtcaaca
    11521 cgtaacgctt tacaggtgat tatcatgggg ctttttgatg aagttgtcgg cgcctttctg
    11581 aaaggcgatg cggggaaata tcaggcgatt ttaagttggg ttgaggagca gggtggcatt
    11641 caggtgctgc tggaaaaact gcaaagtggc ggcttagggg ccattctctc aacctggctg
    11701 agtaatcaac agggcaatca accagttagc ggcgagcaac tggaatcagc actcggcaca
    11761 aacgcggtat ccgatcttgg acaaaaactt ggcgtggata ccagtacggc ttcctgttta
    11821 ctggcagaac aattgccgaa gattattgat gcgctctcac cgcaaggtga agtgtcacca
    11881 caagccaata acgatctgct ttccgcaggc atggaactgc tgaaagggaa actcttccgc
    11941 taagcaaatg gggagcatgc aacccgcgtt gcatccccct tttccctcgg gtgttgtact
    12001 gatttttgag cggaatcgcg ttagcatggg tcaggaacca atctacctgg ggaactcatg
    12061 gctatcaaac tcattgctat cgatatggat ggcacccttc tgctgcccga tcacaccatt
    12121 tcacctgccg ttaaaaatgc gattgccgca gctcgcgccc gtggcgtgaa tgtcgtgcta
    12181 acgacgggtc gcccttatgc aggtgttcac aattacctga aagagctgca tatggaacag
    12241 ccgggcgact actgcattac ttataacggc gcgctggtac agaaggccgc tgatggtaac
    12301 accgtggcgc aaactgctct cagctatgac gactatcgtt tcctggaaaa actctctcgc
    12361 gaagtcggtt ctcatttcca cgccctggac cgcaccacgc tgtacaccgc caaccgtgat
    12421 atcagctact acacggtgca tgaatccttc gttgccacca ttccgctggt gttctgcgaa
    12481 gcggagaaaa tggaccccaa tacccagttc ctgaaagtga tgatgattga tgaacccgcc
    12541 atcctcgacc aggctatcgc gcgtattccg caggaagtga aagagaaata taccgtgctg
    12601 aaaagtgcgc cgtacttcct cgaaatcctt gataaacgcg ttaacaaagg tacgggtgtg
    12661 aaatcactgg ccgacgtgtt aggtattaaa ccggaagaaa tcatggcgat tggcgatcag
    12721 gaaaacgaca tcgcaatgat tgaatatgca ggcgtcggtg tggcaatgga taatgctatt
    12781 ccttcggtga aagaagtggc gaactttgtc accaaatcca accttgaaga tggcgtggcg
    12841 tttgctattg agaagtatgt gctgaattaa tctgtgggcg ggcaaacagc ataaacgcgt
    12901 ttgcccgctt actgattatt ggtttactgc atatacaacc agaaattgca gcggcgcagt
    12961 aatgatgtcc agcgtaacag tcactggcag taatgctgcg gaaacaccat ccggaatgcc
    13021 tctgggtgaa tagtattcat aaaatcgcac tttaaatgga tggtagaacg ccatcacctt
    13081 tgagtaatcc atgtttttat ttttctcatg gattgtgcct tttaggtttt ccagtgacct
    13141 ggtacagtgt tgaacaccac aggcaaaacc ataatgcgtt gccagtgcct tttcttcttc
    13201 gttattccag tagtagtaaa gcgatattgt gccggtaaat ttctttttcc ccgcatttaa
    13261 aacaaatctt gcgtcattgg caacctgaat attgtgccgg ttaagtgctg gatcattgag
    13321 catcttaacg atgtcatcgg caccatcagt gataaggtaa tccagcgatt cccctacaaa
    13381 aacatagcct tttgttccat tactatcttt tgcctgcgat agcccagtga tggtgtctga
    13441 ctgcgattga tactttcctg aactaccctt tgcggtatgg gaaaccagag catacgagat
    13501 acagcctgaa agcatcagcg ccagtgggaa taaactggca gccttaaaaa atcccttaaa
    13561 attcaacttc atgattaatc ctttaaacga gtagctaagc cgttgaaagc atactcttct
    13621 ttccctcctg tttagcgcct gatctccctt ttcagatttc tgcccgatat aagacctttt
    13681 cttatgaggt gtttttttgt ggactaatat ctgttgcaag gctatgggtt cgcacctgca
    13741 ctggtttata tctcctccgt cgcgttgatt ggcgcgctct cttacatcct gctggtgggc
    13801 gatgtgacgc gcgttggcta atctttcaac tgtggaatat gcgccatacc actgcgcata
    13861 atccactttc ttaaagccgg tgcggtcagg catcggcttt ttactatctt gccgaagagt
    13921 tttaaacgca gtttgcgggt ggcgtgccag taggctgcgt tgatcttctc tggctgcttc
    13981 agtaacaccg aacgcagaat ctctgcgcta tccagcgcat agcttatccc ctccagcgag
    14041 ctggcgctga taaatcccgc cgcttcacca atcagaaagg cgttgtcctt accgcagaca
    14101 aaatcctgcc agcgtgaggg aaatagcacc gtgcattttt cgctttttac cgccttacca
    14161 aactggaatt gaaaggcgct cattttctct ttcagcgtcg tgaaacgcgt ctgaccgtct
    14221 ttcattggat aggcaccgcc aaagataaaa taaccgtctt tgctgatact ccatgagtaa
    14281 cagtcagtta tcgcattatc aaagatgcat gagtagaacg gcaccggatg tttctctgcg
    14341 aaccactgct ggatagcgac atatttacga atctgatggt ccgggtagag atatcgccgc
    14401 accatcgagt tggcaccatc tgcaccgacc agatagcggg cagtaatatg ctgctcccaa
    14461 ccgtctgcac gaaaaatgac atgccattta tcatcctcac gccagatttt ccggcacagg
    14521 ctatcgtgat aaacctcaac gctggcgggg atcagcgatt tcatccacaa gtcgaaagca
    14581 tggcgattaa tattgatata gcttcgctgg tagttacgcg tgagcgatgc ggcgacgtcg
    14641 acagttttga cgctgaaaat ctgtggattg gcgatcacat cgacaggaag cgtcagtcca
    14701 tcgcgaataa aagatcgctg cgcgtccggt gccagcagac cgccacaagg cttgctgaaa
    14761 ccttcagtac catgctggtg ctttttatcc agcgcgatca cctgcatttt gccggctaac
    14821 tttcgtgcca acgccgaccc tgccgggccg aggccaataa tcgccacgtc gaaatgttcc
    14881 atcttttcac tcacattcat cacgatggca aaagactatt gtctgaaagt gaagtccttg
    14941 tgaagtatga tggcgggaac cgttttgtat caggaccgcg ccatgaaaca gataaccttt
    15001 gctccccgta atcacctgct caccaatacc aatacctgga cgcccgacag ccagtggctg
    15061 gtatttgacg tgcgtccttc tggcgcgtcg tttaccggcg agaccattga gcgtgtgaat
    15121 atccataccg gcgaggtcga ggttatctat cgcgtgtcac agggcgcgca cgtcggcgtg
    15181 gtgaccgttc atccaaagtc agagaaatat gtctttatcc acggacctga aaatcctcat
    15241 gaaacatggc attacgattt ccatcaccgt cgcggcgtga ttgttgaagg cggcaagatg
    15301 aacaatctcg atgcaatgga tattaccgtt ccgtacacat caggggcgct gcgtggcggc
    15361 agccatgtgc atgtctttag cccgaacggt gaaatggtga gttttaccta taacgaccat
    15421 gtaatgcatc agttcgattc ggcgctggat ttgcgaaacg tcggggttgc tgcaccgttt
    15481 ggcccggtca acgtacaaaa gcagcatccg cgtgaataca gcggtagcca ctggtgcgtg
    15541 ctggtgagta aaaccacgcc cacgccgcag cctggcagcg atgaaatcaa tcgtgcttat
    15601 gaagaaggat gggtaggaaa tcacgcgctg gcgtttattg gcgacacact ttcgccaaag
    15661 ggcgagaaag tgccggagct gtttatcgtt gagttaccgc aagatgaagc tggctggaaa
    15721 gcagcaggtg atgcgccgtt aagtggaacg gaaacaaccc tgcccgcgcc accgcgtggc
    15781 atcgtgcagc gacgtttaac ctttacccac catcgggctt atccggggtt agtcaacgtc
    15841 ccgcgccact gggtgcgctg taatccgcag ggtacgcaaa tcgcgttttt aatgcgagat
    15901 gataacggca ttgtgcaact gtggcttatc tcgccacagg gcggcgagcc gcgccagtta
    15961 acccataaca aaacggatat tcagtctgca tttaactggc atccgtcagg agaatggttg
    16021 ggctttgtgc tgggtaatcg aattgcttgt gcccatgcgc aaagcggcga ggttgagtat
    16081 ttaaccgaaa accacgccaa tccaccttct gcggacgccg tggtcttctc gccggatggt
    16141 cagtggctgg cgtggatgga aggcggccag ctgtggatca ccgaaactga tcgctaaatc
    16201 agttatcagg cattctggcc ggtggaataa ccgcattggt ggcttgcgcg ttggcttcgc
    16261 ttttttcaac gcgtgagcgc accgagctgt ctttgcggaa cacatcccac ggtaataaaa
    16321 gcgtgtccat cacagcggta aacggcatat cgagaatcgc cagcgactta gtgccccagt
    16381 tggtttcatc atcgccaatc atggtggcgc tggcgcgggt gccgggatac gttccttctt
    16441 tccctccggt atgggacatc acgctggagc atcctccgag taatgccatt ccgctgcata
    16501 tcatgaacgc taacaacaca tttcttatca taataaattc atctgttgat cgtgggtgtt
    16561 ggcctgatga gttatagcga tcccttgctg aaaataacat catcattacg tcgctctgtg
    16621 gcggctatcg cactttaacg tttcgtgctg ccccctcagt ctatgcaata gaccataaac
    16681 tgcaaaaaaa agtccgctga taaggcttga aaagttcatt tccagaccca tttttacatc
    16741 gtagccgatg aggacgcgcc tgatgggtgt tctggctacc tgacctgtcc attgtggaag
    16801 gtcttacatt ctcgctgatt tcaggagcta ttgattatgc gtaactttga tttatccccg
    16861 ctttaccgtt ctgctattgg atttgaccgt ttgtttaacc acttagaaaa caaccagagc
    16921 cagagtaatg gcggctaccc tccgtataac gttgaactgg tagacgaaaa ccattaccgc
    16981 attgctatcg ctgtggctgg ttttgctgag agcgaactgg aaattaccgc ccaggataat
    17041 ctgctggtgg tgaaaggtgc tcacgccgac gaacaaaaag agcgcaccta tctgtaccag
    17101 ggcatcgctg aacgcaactt tgaacgcaaa ttccagttag ctgagaacat tcatgttcgt
    17161 ggtgctaacc tagtaaatgg tttgctgtat atcgatctcg aacgcgtgat tccggaagcg
    17221 aaaaaaccgc gccgtatcga aatcaactaa tttcctaagg ccgccttgcg cggcctgaca
    17281 tctccatgct cgccgtcagg gagcatatgc gaatcttcgg atttgcaggt acttactcgc
    17341 ttcttagaag gagaaatgac tatgcgtaac ttcgatttat ccccactgat gcgtcaatgg
    17401 atcggttttg acaaactggc caacgcactg caaaacgccg gtgaaagtca gagcttcccg
    17461 ccgtacaaca ttgagaaaag cgacgataac cactaccgca ttacccttgc gctggcaggt
    17521 ttccgtcagg aagatttaga gattcaactg gaaggtacgc gcctgagcgt aaaaggcacg
    17581 ccggagcagc caaaagaaga gaaaaaatgg ctgcatcaag ggcttatgaa tcagccattt
    17641 agcctgagct ttacgctggc tgaaaatatg gaagtctctg gcgcaacctt cgtaaacggt
    17701 ttactgcata ttgatttaat tcgtaatgag cctgaaccca tcgcagcgca gcgtatcgct
    17761 atcagcgaac gtcccgcgtt aaatagctaa ctagctattc tctttgcccc gccattctgg
    17821 tggggctttt tttgtctttc ttcttcaaca atgcgttaaa tttcgactgt ttaagatatt
    17881 tcggcacgtt ctttcccgtg ataatgtgcg ctataaggca aatctgcttc accgattcag
    17941 ccataatccg tagcaattaa aatgttatct caagagaaat ggacgatgag tgatatagca
    18001 ttaacggtca gtattttggc tttggtggca gtcgtcggtt tgtttatcgg caacgtcaaa
    18061 tttcgcggca taggattagg tattggcggc gtgctgtttg gtgggatcat cgtcggccat
    18121 tttgtttctc aggcggggat gacattaagt agcgatatgc tgcatgttat tcaggaattt
    18181 ggcctgatcc tgttcgttta taccatcggg attcaggtag ggccgggctt ctttgcctca
    18241 ttgcgcgtct ccggattacg cctcaacctg tttgctgttc tgatcgtcat catcggtggt
    18301 ctggttaccg ccatcctgca taaactgttt gatattccac tgccggtagt gctggggatt
    18361 ttctccggtg cggtaaccaa tacgccagcg ctgggggcag ggcagcagat tttgcgcgac
    18421 ctgggtacac caatggaaat ggtcgatcag atggggatga gttacgcgat ggcgtatcca
    18481 ttcggcattt gcgggatatt gttcaccatg tggatgttgc gggttatttt ccgcgtcaat
    18541 gtcgagacag aagcccagca gcacgagtct tcacgcacca atggcggcgc gctgatcaag
    18601 actatcaata ttcgcgttga gaaccctaac ctgcatgatt tagccattaa agatgtaccg
    18661 attctcaacg gcgacaaaat tatctgctcg cgtctgaaac gcgaagaaac cctaaaagtt
    18721 ccttcgccag ataccattat ccaactgggc gatttgctgc atctggtggg tcagccagcg
    18781 gatttacata atgcgcaact ggtgattggt caggaggtcg atacttcgct gtccacgaaa
    18841 ggcactgatt tgcgcgtcga gcgtgtggtg gtcaccaatg aaaacgtgct tggtaagcgt
    18901 attcgcgacc tgcattttaa agaacgctat gacgttgtta tctcgcgcct gaaccgtgcc
    18961 ggggtcgaac tggtcgccag tggcgatatc agcctgcagt tcggcgatat cctcaacctg
    19021 gtggggcgtc cgtccgcaat tgatgccgtt gccaatgtgc tggggaatgc gcagcaaaaa
    19081 ctgcaacagg ttcagatgct gccagtgttt attggcatcg ggcttggcgt attgttaggt
    19141 tctattcccg tctttgtgcc aggattcccg gccgcgttga aactggggct ggcgggcggt
    19201 ccgctgatta tggcgttgat cctcgggcgt atcggcagta tcggcaagct gtactggttt
    19261 atgccgccaa gtgccaacct cgcgctgcgg gaactgggga tcgtgctgtt cctctcggtc
    19321 gttggtctga aatctggtgg ggattttgtg aataccctgg tcaatggcga agggctaagc
    19381 tggattggtt atggtgccct gatcaccgcc gttccgctga ttactgttgg cattctggcg
    19441 cggatgttag ccaaaatgaa ttacctgacc atgtgcggga tgctggcagg ttccatgacc
    19501 gatcctccgg cgctggcgtt tgctaataat cttcatccaa ccagcggtgc ggcggcgctc
    19561 tcttacgcca ctgtctatcc gttagtgatg ttcctgcgca ttatcacccc ccaattactg
    19621 gcggtgctct tctggagtat cggttaactc ttgtccgggt ggagtcgccg taaatggtac
    19681 tccacctggt attcgctggc atttctgaac atcactgaat aattcaaaaa ctccccgctc
    19741 tcgctatatg acaacgaggt gatccgcagc agtggcgtgt gttccgctac cttcatttgc
    19801 cgcgccagca gtctatcggc gagcaccggc gtcaggcttt cataattacc gccaatcaaa
    19861 atcccgcact cttgttcaat atattcaaac ttcgaccctt ccagatgttg cagcgaaagg
    19921 ttacggaaca gttttaccgg catatagctg tcttccagca tcagcggttt cccttccaca
    19981 aaacgaacgc gacgggagaa gtagatctgc tcgttgattt gaatccgcaa ctggctggca
    20041 attgccggag gcgcaggcat gatttcaaaa atcagcacct ggctggtgac tgtttttccc
    20101 tgccgtttta acacctccac cagcccggtc aggctggcgg tttgatgcag cacatctttg
    20161 cgcaccaggt aagtgccgct gccgtggcgg cggaccacca gcccccacgc taccagcagg
    20221 tcaatcgctt tacggatggt catccgcgat accgcaaact cttccgccag ctttttttca
    20281 ccgggaagaa ggctgtttag cgtgaaatcc gcggaattaa gtcgaattct taaccgctcc
    20341 gcaatgcttt tgtagatcat ctctagatct cttttttgcc tgcaagcccg ggtaatgcgc
    20401 ccacaagttc tgcttaatca attgaaaaaa catctttttt ttaaagatgt gttcgatgct
    20461 gtgtaccagt gctcacagag atctactttt tcgcgaaaaa gtagatctct accgcccaac
    20521 gaaaagcatg aaagcgatca cgaatcccat tgggtcgtca tgttctgccg atcgcatctt
    20581 cctatcctcg ctccaggcct gccgcataac caatcaggct tcctacttac agaattgaga
    20641 aaagaggatg tggaaatgct cagtcaaatt caacgctttg gcggcgcgat gttcacgcca
    20701 gtgctgctgt ttcccttcgc cgggattgtg gtgggtcttg ccatcttgct gcaaaacccg
    20761 atgtttgtcg gggaatcact gaccgatccg aacagtttat tcgcgcaaat cgtacacatt
    20821 attgaagagg gcggttggac ggtattccgt aatatgccgc tgatttttgc tgtcggttta
    20881 cccattggcc ttgctaagca agcgcagggg cgtgcttgtc tggcggtgat ggtgagtttc
    20941 ctgacctgga actatttcat caatgcgatg ggaatgacct ggggaagcta cttcggcgtc
    21001 gatttcactc aggacgcggt ggcaggtagc ggtctgacaa tgatggccgg gattaaaacc
    21061 ctcgatacca gcattatcgg cgcaattatc atttccggca ttgtgacggc gctgcataac
    21121 cgtctgttcg ataaaaaact gccggttttt ctcggcattt tccaggggac gtcttatgtg
    21181 gtgattatcg ccttcctggt gatgatcccc tgtgcctggc tgacattgct cggctggcca
    21241 aaagtacaaa tggggattga atctctgcaa gcgttcctgc gttcggcggg tgcgcttggg
    21301 gtgtgggttt acaccttcct cgaacgtatt ctgatcccaa ccggtttaca ccacttcatc
    21361 tacggaccgt ttatctttgg tccggcagct gttgaaggcg gaattcagat gtactgggcg
    21421 cagcatctgc aagagttcag tttgagcgcc gagccgctga aatcgttgtt cccggaagga
    21481 ggttttgccc tgcacggtaa ctcaaaaatc tttggtgccg tgggcatttc tttagcgatg
    21541 tacttcactg ccgcaccgga aaatcgggta aaagtggcgg gtttgctgat tcccgcaacc
    21601 ttaaccgcca tgctggtggg aattaccgaa ccgctggaat ttaccttcct gttcatttca
    21661 ccgttgctgt ttgcggtaca cgctgtgctt gcggcctcaa tgtcgaccgt gatgtatctc
    21721 tttggtgtgg tgggcaacat gggcggaggt ctgattgacc aggttttacc gcaaaactgg
    21781 atcccgatgt tcagcaacca cgcggatatg atgctgaccc aaatcgccat tgggttgtgc
    21841 tttaccctgc tgtacttcgt ggttttccgc accctgattc tgcaattcaa catgtgcacg
    21901 ccgggacgtg aagatgcgga agtgaaactc tactcaaaag ccgaatacaa agcctcgcga
    21961 ggccaaacca ccgcggcaga gccaaaaaaa gagctggatc aggctgccgg tatcctgcaa
    22021 gccctgggcg gggtcggcaa tatctccagc attaacaatt gtgcgacgcg tctacgcatt
    22081 gcactgcatg acatgtcaca aacgctggat gacgaagtct ttaaaaagct gggagcgcac
    22141 ggcgtcttcc gtagtggcga tgccattcag gtgatcattg gtctgcatgt atcccagctg
    22201 cgtgaacagc tcgatagctt aattaattct catcaatcag cagaaaatgt tgccattacg
    22261 gaggcagtat aatgaccaaa ttctcagtgg ttgtcgcagg cggtggaagc acctttacgc
    22321 caggcatcgt gttgatgctc ctggcgaatc aggaccgttt cccgcttcgt gcactgaaat
    22381 tttatgataa cgatggttcg cggcaggaag tgattgccga agcctgtaaa gtcatcctta
    22441 aagaaaaagc gccggatatt gcgtttagtt acaccaccga tcctgaagtg gcgttcagcg
    22501 acgttgattt tgtcatggcg cacatccgcg tcggcaaata cccgatgcgc gaactggatg
    22561 aaaaaatccc gctgcgccac ggcgttgttg gtcaggaaac ttgcggaccc ggcggaatag
    22621 cgtacggcat gcgttccatt ggcggcgtcc tggaactggt ggattatatg gaaaaatatt
    22681 caccaaacgc ctggatgctc aactactcca acccggcagc aattgttgca gaagccacgc
    22741 gtcgtctgcg cccgaatgcg aaaatcctca acatctgtga catgccaatc ggtattgaaa
    22801 gccggatggc gcaaattgtt gggctgcaag atcgcaaaca gatgcgcgtg cgctactacg
    22861 gcctgaacca ctttggctgg tggacatcaa ttgaagattt gcagggcaac gacctgatgc
    22921 accagctgcg gcaatatgtc tctaagcacg gttatgttcc accgcagcaa gatacacata
    22981 ctgaagcgag ctggaacgac acctatgcaa aagcgcggga tgtccaggca ctggacccgg
    23041 atacattgcc gaacacctat ctgaaatatt atctcttccc ggattatgtc gttcagcatt
    23101 ccaaccctga acatactcgc gcgaatgagg taatggaaca tcgcgagaaa caggttttcg
    23161 atgcttgccg cgccattacg gcggcaggaa attcagcggc gggcaagctg gaaattgacg
    23221 aacatgcgtc atacatcgtc gatctggcgg cggcaattgc cttcaacact caggagcgga
    23281 tgttgctgat tgtgcctaac aacggggcaa tccataactt tgatgatgaa gctatggtcg
    23341 agatcccgtg tctggtcggg cacaacggac cagaaccact ggtggtcggc gatatcccgc
    23401 agtttcagaa agggttaatg agtcagcaag tggcggtgga aaaactggtc gtggacgcct
    23461 gggaacagcg ttcatatcag cacctgtggc aggcgattac gttgtcgaaa acggtaccga
    23521 gcgcctcggt cgccaaagct attctggatg agttgctgga ggccaacaaa gcgtactggc
    23581 cagagttacg ttaattgtgt ggactggcat ccgttgggtg ctggtccgta ttctttagca
    23641 cggctgaagg cgcataacca aatttatgtt taaaggcttt gctgaaatga aaggagtcga
    23701 agaagttgag cgtatcagcg acttcgccaa ccgagtttcc ttgttgcttt aatagcgata
    23761 gcgccagatc cagacgcgca tcgaggtaat attctttcgg cgtctttccg gtatagcgta
    23821 aaaacagacg acgcaaccag gcttcgctac aggggatcgt ggcagccata tcggctacgc
    23881 tccagcgttg ttgcagactg gcatgtaaag tggcaattaa tttttcaatt tgccgccgtt
    23941 gtggatcttt tttaccgtct gcgtacataa gacaaatcca ctgataaata attttcgtca
    24001 gaaaagcgac tgccagatta ttttttattg cttctggtga agttattaac tcagcaactt
    24061 cggtgagttc ctgattataa atttcgccat tgtaaataac gctttgctga ccgatgggaa
    24121 tatccatcat actggtgggg gtaaattcca tccagtattg ttcccagact aaaccttcac
    24181 agtgataaga gtgaatatcc attggcttta aaaatataat acagttgcca tgcagtgtta
    24241 tttgttcgcc attctttaaa agaatcttcc cacatccttg tacggtatac accgcaaccc
    24301 atgagttggc gataagcggt ttcttcttgt cccgtggcca gatcacccgg taatgctcgt
    24361 cggcgtaggt atgccagagc gagatcagag aaattccgct ggagatcaca ttttcgttaa
    24421 tcaataaagg aatattgtat ggagtttcgt tttttacatc tgaagtttgc aattttccat
    24481 tcattttaat atccctccgg cgaattatgc tcacaacctg aacttaacag aaaacaatcc
    24541 gttcagggat acgacgtttg tattttaaga atctgactgc ctgacccgac gcattttacc
    24601 tctccctata ttcatgcgtc cgggacataa acataaataa gggctatgag atgaattcgt
    24661 tacaaatctt gagttttgtc ggttttacgc tgctggtggc gatcatcacc tggtggaagg
    24721 ttcgcaaaac agataccgga tcgcaacaag gctattttct tgccggacgt tcactaaaag
    24781 cgccggttat tgccgcttcg ttaatgttaa ccaacctttc cacggaacaa ctggttggtc
    24841 tttccgggca ggcctacaaa agcggcatgt cggtgatggg ctgggaagtg acttcagcgg
    24901 tgacgctgat cttcctcgcg ctaatctttt taccgcgcta tctgaagcgc ggcattgcca
    24961 ccatccccga ttttctggag gaacgttatg ataaaacgac gcgtatgatc atcgacttct
    25021 gcttcctcat tgccaccggc gtctgctttc tgccgattgt tctctactcc ggcgcgttgg
    25081 cgctcaacag cctgtttcac gtcggggaat cgctacagat ttctcacggt gcggctatct
    25141 ggctactggt aattttgctt ggtctggcgg gaattttgta tgcggtgatc ggcggactgc
    25201 gcgcaatggc agtggcggac tccatcaacg gtattgggct ggttatcggc gggttgatgg
    25261 tgccggtatt tggcttgatc gcgatgggca agggcagctt tatgcagggc attgagcaaa
    25321 ttaccaccgt tcacgccgag aaattaaact cagtcggtgg cccgaccgat cccttgccga
    25381 ttggcgcggc atttaccggt ttaattctgg tgaacacctt ttactggtgt acaaatcagg
    25441 gcatcgtgca acgcacgctg gcgtcaaaaa gcctggcgga agggcaaaag ggggcgctgt
    25501 taacggcggt gctgaaaatg ctcgacccgc tggtactggt gctgccaggg ttgattgcgt
    25561 ttcatctgta tcaggatcta ccgaaagccg acatggccta cccgacgctg gtcaataacg
    25621 ttctgccagt gccactggtg ggtttcttcg gcgcggtgtt atttggtgca gtgatcagta
    25681 ccttcaacgg ctttctgaat agcgccagta cgttattcag tatgggtatt taccgtcgca
    25741 tcattaacca gaatgccgag ccgcagcagc tggtcaccgt cgggcgcaaa tttggtttct
    25801 ttatcgctat cgtttcggtg ctggtagcgc cgtggatcgc caacgcgccg caggggctgt
    25861 atagctggat gaaacagctc aacggcattt acaacgtgcc gctggttacc atcatcatta
    25921 tgggcttttt cttcctgcgc atcccggcgc tggcggcaaa agtggcgatg gggattggca
    25981 taatcagcta catcaccatc aactatctgg tgaagttcga cttccatttc ctctatgtgc
    26041 tggcctgtac gttctgcatc aacgtggtcg tgatgctggt gatcggtttt atcaaaccgc
    26101 gcgccacgcc gttcaccttc aaagatgcgt ttgcggtgga catgaaaccg tggaaaaacg
    26161 tcaagatcgc gtcaattggc atcctgttcg cgatgattgg cgtctatgcc gggctggctg
    26221 aattcggcgg ctacggtacg cgctggttag cgatgatcag ttatttcatt gccgccgtag
    26281 tgattgtcta cctgattttt gacagctggc ggcatcgtca cgacccagcc gtaaccttta
    26341 ctcccgacgc gaaggatagc ctatgaaacg ccccaatttt ctgttcatca tgaccgatac
    26401 ccaggccccc aatatggtcg gttgctatag cggtaaaccg ctgaatacgc aaaatattga
    26461 tagtctggcg gcggaaggta ttcgctttaa ttccgcctac acctgttcac cggtttgtac
    26521 accggcgcgc gccgggctgt ttaccggtat ctacgctaac cagtccgggc cgtggaccaa
    26581 caacgtcgcg ccgggtaaaa acatctccac tatggggcgc tactttaagg atgccggcta
    26641 tcacacctgt tacatcggca aatggcatct cgacggtcat gactatttcg gcactggcga
    26701 gtgtccgccg gagtgggacg ctgattactg gttcgatggg gcgaactatc ttagcgaact
    26761 gacggaaaaa gagattagcc tgtggcgcaa tggcctaaac agcgtcgaag atttacaggc
    26821 gaaccatatt gacgaaacct tcacctgggc gcaccgcatc agcaatcgag cggtggattt
    26881 tctgcaacag cccgcgcgcg ccgaggaacc cttcctgatg gtggtttcgt atgatgagcc
    26941 gcatcacccg ttcacctgtc cggtggagta tttagagaaa tacgctgatt tttactacga
    27001 gctgggcgag aaagcacagg atgacctggc gaacaaacca gaacatcacc gcttatgggc
    27061 gcaggcgatg ccatcgccag tcggtgatga cgggctttat caccatccgc tctattttgc
    27121 ctgtaatgac tttgttgatg accaaatcgg acgggtcatc aatgccttaa agccagagca
    27181 acgtgaaaat acgtgggtta tttatacctc cgatcacggc gaaatgatgg gcgcacataa
    27241 gctgatcagt aaaggggcgg cgatgtatga cgacatcacc cgcattccgc tgatcatccg
    27301 ttcgccgcaa ggggagcggc gacaggtcga tacgccagtc agtcatatcg atttactgcc
    27361 gacaatgatg gcgctggcag atattgaaaa accagagatt ctgccggggg aaaatatcct
    27421 tgcagtgaaa gagccacgcg gcgtgatggt ggaatttaac cgctacgaga ttgagcatga
    27481 cagctttggc ggttttattc cggtgcgttg ctgggtgacg gatgacttta aactggtgct
    27541 caacctcttc accagtgatg aactttacga tcgccgtaat gatccaaatg aaatgcataa
    27601 cctgatcgat gatgcccatt ttgccgacgt tcgcagcaaa atgcatgatg ccttattgga
    27661 ttatatggac aaaattcgcg atccgttccg cagttaccaa tggagcctgc gtccgtggcg
    27721 taaagatgca cagccgcgct ggatgggggc atttcgtcca cgcccacaag atggttattc
    27781 gccagtggta cgcgactatg acaccggcct gccgacacaa ggggtgaagg tggaagagaa
    27841 aaaacagaag ttctgacgat aatgcccgct gatgaacacc cggcgggcat tattcaggct
    27901 gcttaaacca gtgaaccgcc agtcaggaag gaaaagataa ggaatatcag caaaatgctg
    27961 cacacgatgc cgataccaaa aagcaaggta tggaaagcca gcgtcccgcc gccaaaaacc
    28021 cgcgcgccgc gaatgcccca gcgattttcc ttacgcatac cgccaataaa tgcgcaaagt
    28081 agcgcagcaa tggccagtgc gataccgctt tgatcgagga ttttatcgac atccacgtcc
    28141 tcttttctct ccacggtggt aattttttcg ccttttattc cggcgagaag acctctttta
    28201 atttcagccg ttttgtccgc cacgatgctt tcaagcgttg gcggtggtgc ggaaaaaggg
    28261 ccgaatgaaa agtggataat ccccatcatt aaagcgatgg caccgaatag catgcccaat
    28321 gaactgattt tattttgcgc tatgataccc atctgacctc cttgtagcgg atggcaaaag
    28381 catatcagga aacgggcgga cgaaattctt aaaaaaaacc gctaaaggta gagcgttagt
    28441 gaaattgcaa aggagcaaca atgaaaattt cccgcctcgg agaagcgcct gattaccgct
    28501 tctcgctggc taatgagcgt acttttctgg cgtggatccg tactgcacta ggatttctgg
    28561 cggcgggggt tgggcttgat caacttgcgc cagatttcgc cacgcccgtc attcgcgaac
    28621 tgctggcgtt gttgttgtgt ctgttttccg gcgggttagc gatgtatggc tacttgcgct
    28681 ggttacgcaa tgaaaaagcg atgcgtctga aagaggacct gccctacacc aacagcttat
    28741 taatcatcag cttaatattg atggtcgtag cagtgatcgt tatgggactg gtgttgtatg
    28801 ccggatagcc gcaaagccag acgcattgcc gacccagggc tgcagccgga gcgtacatca
    28861 ctggcgtggt ttcgtaccat gctgggctac ggcgcgttga tggcgttggc tatcaaacac
    28921 aactggcacc aggcgggcat gttattctgg atttcgattg gcatcctcgc catcgtggcg
    28981 ctgatcctct ggcactacac tcgtaatcgc aatttaatgg atgtcacgaa tagcgatttt
    29041 tcccaatttc acgtagtccg tgacaaattt ttgatctccc tcgcggtgtt atctctcgca
    29101 atactgtttg ctgtaacgca tatacatcaa cttatcgtat ttattgagag agtcgcatga
    29161 caggaagtca ggtcattgat gctgaagagg acaggcataa actggttgta gagtacaagg
    29221 atgctctgca acctgccgat ttttatcaca atttcaaaca acggggcatc cgctccgtac
    29281 agcttattcc ccatcttgaa tttgatgacc ggggcgatat gacggccgct tcggtgacag
    29341 cagagctgtg ggggaaattt ttaattgccc tgtttgagtg ttgggtgcgg gcagatatca
    29401 gtcgtatctc gattgagctt tttgacgcca cattacaaaa atggtgtggc agcgaaaacc
    29461 cgcaaccgcg ccgtggctgc caggcgtgcg actggcatct gctgtgtccg cacgctcggg
    29521 aggagacgcc agacagcgtg ctttgtgcgg gctatcaggc gttttattct tactctgcgc
    29581 cgcatatgcg ggtgatgcgt gatctgataa agcagcatcg ttctcccatg gagctgatga
    29641 cgatgctgcg gtgacgtgag cttaaacggg ctgcccctga tgcgacatcc gcgtcgccag
    29701 cggtagccag cacagcacga tcaacaatcc cattaaggtc atcaacaacc ccaggctgcc
    29761 ctgaccggtt tgcggcaaca tcgcagagag cgacgccagc acgccggaac caatgttttg
    29821 tagaccgccg accagcgcgc cagccgtgcc cgccaggaaa gggaacggct ccatcgcgcc
    29881 gctggtcgcc agcggaaaca gcatcccggc accgaaaaag aacagcgcgg cgggaacgag
    29941 cagcgtccag acattcatca cgccaaacca gtcggggatc cacatcagta agccagccag
    30001 caggcagcag ataaccgact gccacatcaa cgttgagaag cgtttattag gacgtccggc
    30061 aaaccatgcc ccaaaaaatg ccgccggaat cggcagaata aacaaaatac tgaccgtcat
    30121 actgctcagc cctaacaccg cgcccatcag cacgccggag caggcttcaa aggcggcaat
    30181 cccggccaga ccgccaatca gcatcagcaa ataacagtta aaaccgctgt taccgaaaag
    30241 cgttttataa ctggtaagca ggcgcgtgcg cggtgcgtcg accggacgcg tttccggcat
    30301 ccagcgggcc atactgaagg tcacaccggc acaaagtacc aacaaaaaga gataacaggc
    30361 gcgccagttc cacatcgtat ccagcagacc gccgattagc ggtgcgagca acggactgac
    30421 aagaattccc atgtttaaca ggctgttagc gtggcgcaac tgtgtccgtt catataaatc
    30481 acgcggcaaa gtacgcgcca ttacgccgcc aacgccggtg cccatcccct gcatcgcgct
    30541 ggcggcaatc aatactgtca aactggaggt cgtgaccgcg accagcgttg ccagcataaa
    30601 aatggacatt ccgacgagga tcaccggtcg gcgacccaca cggtcggaaa tcgggccata
    30661 aaacagctgt gagacaccgt aagtcagcag ataagcgccc attacgctct gcaccgcccc
    30721 ttcacgaacg ttgagatcgc gcgccatatc ggcaatagct ggaatataaa tggtttgcgc
    30781 catctgaccg acggccacga gtaataccaa catcaataac aaattgatgt ttctatgcct
    30841 tttcattatc acggatgctt ttataaaaat aattagaata agtcactacg cattcccata
    30901 aatgcgggag aggaatctat catattgata aatgttagcc agatatacgc caggaatggg
    30961 gaattgttta gcggcagaat atgtaaacaa aagcggcaat aaatgttgcc gggagggatg
    31021 gtaggtcgga taagatgcgt cagcatcgca tccgacacca acccacacgc taaatataaa
    31081 atgggagcgg ttacccgctc ccctttagtg cgacttgaat ctgaattact tcaggtattt
    31141 cagaacagca tcaagcagtt gcagtgctgc aacaatgagt ttgaggataa gaatggcgat
    31201 atccaccagg ctcatacgcg tctcctgtgg ttcaggagca ctgccagctg acgtaccttt
    31261 ccgctgcctg ttgccagctg ttgtgcacgc ggtatctcaa ggagaaacgt tgtgctcgtt
    31321 gacataacac agtgtgctca cgcggggtta aggcgctgac ggcaccaccc gtttcagcca
    31381 ggacttagtg cgccgggtaa cgatgcggcc cccgcataac acattgcgta cagtgatatt
    31441 atagaagctt gctgtataaa taaacagtaa tatttggaca aaacgcaaac tgtgatcgat
    31501 actcaactat gcactaaata cgtcaaaatt cgtgccgaaa ttgcgcgttt tgcgcggaac
    31561 acgtatactt tctgtgttga cataatacag tgtgctttgc ggttaccagc cgcgggcggc
    31621 tgacgaaacc tcgctccggc ggggtttttt gttacctgca gtacagtaca aaacgtgatc
    31681 aacccctcaa ttttcccttg ctgaaaaatt ttccattgtc tcccctgtaa agctgtgctt
    31741 gtataaatat tgttaaacac aaaaccaaca aggtccccaa tggctacttc catgctcaac
    31801 gcaaaactac taccaactgc gccatccgcc gcagtggtcg tcgtgcgtgt ggtggtggtc
    31861 gtcggcaatg cgccgtaggg actggaacaa cacacgattc caaaaccccg ccggcgcaaa
    31921 ccgggcgggg tttttcgttt aagcacctcc cggaaagtcg gcccagaaga aaaggactgg
    31981 agcatggcaa gttcgggcac atcatcgacg cgtaagcgct ttaccggcgc agaatttatc
    32041 gttcatttcc tggaacagca gggcattaag attgtgacgg gcattccggg cggttctatc
    32101 ctgcctgttt acgatgcctt aagccaaagc acgcaaatcc gccatattct ggctcgccat
    32161 gaacagggcg cggggtttat cgctcaggga atggcgcgca ccgacggtaa accggcggtc
    32221 tgtatggcct gtagcggacc gggggcgact aacctggtga ccgccattgc tgatgcgcgg
    32281 ctggactcca tcccgctgat ttgcatcact ggtcaggttc ccgcctcaat gatcggcact
    32341 gacgccttcc aggaagtcga cacctacggc atctctatcc ccatcaccaa acacaactat
    32401 ctggtcagac atatcgaaga actcccgcag gtcatgagcg atgccttccg tattgcgcaa
    32461 tcaggccgcc ctggcccggt gtggatagac attcctaagg atgtgcaaac ggccgttttt
    32521 gagattgaag cacagcccgc ggtggcagaa aaagccgtcg cgcccgcctt tagcgaagaa
    32581 agcattcgtg acgcagcggc aatgattaat gctgccaaac gcccggtgct ttatctgggc
    32641 ggtggtgtga tcaatgcgcc tgcgagagtg cgtgaactgg cggagaaagc gcagctgcct
    32701 accacaatga ctttaatggc gctgggcatg ttgccaaaag cgcatccgtt gtcgctgggt
    32761 atgttgggga tgcacggtgt gcgcagcacc aactatattt tgcaggaggc ggatttgttg
    32821 attgtgctcg gtgcgcgttt tgatgaccgg gcgattggca aaaccgagca gttctgcccg
    32881 aatgccaaaa ttattcatgt cgatatcgac cgcgcagagc tgggtaaaat caagcagccg
    32941 cacgtagcga ttcaggcgga tgttgatgac gtgctggcgc agctgatccc gcaggtggaa
    33001 gcgcaaccgc gtgcagagtg gcaccagttg gtagcggatt tgcagcgcga attcccgtgt
    33061 ccaatcccga aagcgtgcga tccgttaagc cattacggcc tgatcaacgc cgttgccgcc
    33121 tgtgtcgatg acaatgcgat tatcaccacc gatgtgggtc agcatcagat gtggacggcg
    33181 caggcttatc cgctcaatcg cccacgccag tggctgacct ccggcgggct gggcacgatg
    33241 ggttttggcc tgcctgcggc gattggcgcg gcgctggcga acccggatcg caaagtgttg
    33301 tgtttctccg gcgatggcag cctgatgatg aatattcagg agatggcgac cgccagcgaa
    33361 aaccagctgg atgttaaaat cattctgatg aacaacgaag cgctggggct ggtgcatcag
    33421 caacagagtc tgttctacaa gcagggcgtt tttgccgcta cctatccggg aaaaatcaac
    33481 tttatgcaga ttgccgccgg attcggcctc gaaacctgtg atttgaataa cgaagccgat
    33541 ccgcaggctg cattgcagga aatcatcaat cgccctggtc cggcgctgat ccatgtgcgc
    33601 attgatgccg aagaaaaagt gtacccgatg gtgccgccag gtgcggcgaa tacagaaatg
    33661 gtgggggaat aagccatgca aaacacaact catgacaacg tgattctgga actcaccgtt
    33721 cgcaaccatc cgggcgtaat gacccacgtt tgtggccttt ttgcccgccg cgcttttaac
    33781 gttgaaggca ttctttgtct gccgattcag gacagcgaca aaagccatat ctggctactg
    33841 gtcaatgacg accagcgtct ggagcagatg ataagccaaa tcgataagct ggaagatgtg
    33901 gtgaaagtgc agcgtaatca gtccgatccg acgatgttta acaagattgc agtgtttttt
    33961 cagtaaggag cgacattaaa ccgctcgggg gagttactga tgagcgattt ttgatagatc
    34021 cgtgcagata tatagggtga aattgtgcca gagaccatct caggcacaat ttattagccc
    34081 aggacgttaa tttaagtcgc agcaccggta acctgggggg gacagttagg gctaaaattt
    34141 tcagcgatgg tgttaaattc tgattgctgt ctataccagt ggtcatgacc agcattctat
    34201 taattaatgg ttcatcgtga ggtatattat ggcaagtcct ataaacagcg gaataatgat
    34261 ggcaaatctc tgcccttcca cctttttgga taaaaataga aatgtcgctg ctgagcttga
    34321 tattaaaaat aacgagaaaa aatattcacc tggcagtaat ttcgcaaaat ggatgttgca
    34381 ggaaataaag cgcttaatac taaacatcat gtccggttct cggagtatta acaccgatat
    34441 tcttgactat ttccacccta tgccaggcac ggaaaataat ggtaatcgta cctgggtggc
    34501 ggcaactgga gaggatgaat atatcgaaat caaacaaaca ggtgataagt cttttaatat
    34561 tacactggtt ggcagagata aaccttcacg aaaagaaatt ccatattcag gagtggctgt
    34621 agcgactatt attaaatcgt tgtctgaaaa aacttctgcg ttag
//
LOCUS       DAZKIP010000039        34319 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZKIP010000039 DAZKIP010000000
VERSION     DAZKIP010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.039"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(119..794)
                     /locus_tag="ACHOUK_002131"
                     /pseudo
     CDS             complement(119..794)
                     /locus_tag="ACHOUK_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(939..1547)
                     /gene="yiiX"
                     /locus_tag="ACHOUK_002132"
     CDS             complement(939..1547)
                     /gene="yiiX"
                     /locus_tag="ACHOUK_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFP9897584.1"
                     /translation="MKIRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(1607..1924)
                     /gene="metJ"
                     /locus_tag="ACHOUK_002133"
     CDS             complement(1607..1924)
                     /gene="metJ"
                     /locus_tag="ACHOUK_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFP9897585.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            2201..3361
                     /gene="metB"
                     /locus_tag="ACHOUK_002134"
     CDS             2201..3361
                     /gene="metB"
                     /locus_tag="ACHOUK_002134"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFP9897586.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAENPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAKAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            3364..5796
                     /gene="metL"
                     /locus_tag="ACHOUK_002135"
     CDS             3364..5796
                     /gene="metL"
                     /locus_tag="ACHOUK_002135"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFP9897587.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPTSQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(5935..6069)
                     /locus_tag="ACHOUK_002136"
     CDS             complement(5935..6069)
                     /locus_tag="ACHOUK_002136"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9897588.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            6145..7035
                     /gene="metF"
                     /locus_tag="ACHOUK_002137"
     CDS             6145..7035
                     /gene="metF"
                     /locus_tag="ACHOUK_002137"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFP9897589.1"
                     /translation="MNFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            7364..9544
                     /gene="katG"
                     /locus_tag="ACHOUK_002138"
     CDS             7364..9544
                     /gene="katG"
                     /locus_tag="ACHOUK_002138"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFP9897590.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            9638..10543
                     /gene="yijE"
                     /locus_tag="ACHOUK_002139"
     CDS             9638..10543
                     /gene="yijE"
                     /locus_tag="ACHOUK_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFP9897591.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAAVFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKRI"
     gene            complement(10570..11187)
                     /gene="yijF"
                     /locus_tag="ACHOUK_002140"
     CDS             complement(10570..11187)
                     /gene="yijF"
                     /locus_tag="ACHOUK_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFP9897592.1"
                     /translation="MKASLALVSLLTAFTSYSLKSPAIPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYVQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(11461..12564)
                     /gene="gldA"
                     /locus_tag="ACHOUK_002141"
     CDS             complement(11461..12564)
                     /gene="gldA"
                     /locus_tag="ACHOUK_002141"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFP9897593.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENSPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(12575..13237)
                     /gene="fsa"
                     /locus_tag="ACHOUK_002142"
     CDS             complement(12575..13237)
                     /gene="fsa"
                     /locus_tag="ACHOUK_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFP9897594.1"
                     /translation="MELYLDTANVAEVERLAHIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(13249..15750)
                     /gene="ptsP"
                     /locus_tag="ACHOUK_002143"
     CDS             complement(13249..15750)
                     /gene="ptsP"
                     /locus_tag="ACHOUK_002143"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFP9897595.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFSRSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            16059..17138
                     /gene="frwC"
                     /locus_tag="ACHOUK_002144"
     CDS             16059..17138
                     /gene="frwC"
                     /locus_tag="ACHOUK_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFP9897596.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            17153..17473
                     /gene="frwB"
                     /locus_tag="ACHOUK_002145"
     CDS             17153..17473
                     /gene="frwB"
                     /locus_tag="ACHOUK_002145"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFP9897597.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            17524..19821
                     /gene="pflD"
                     /locus_tag="ACHOUK_002146"
     CDS             17524..19821
                     /gene="pflD"
                     /locus_tag="ACHOUK_002146"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFP9897598.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELGQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAASCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFDQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVGNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            19787..20665
                     /gene="pflC"
                     /locus_tag="ACHOUK_002147"
     CDS             19787..20665
                     /gene="pflC"
                     /locus_tag="ACHOUK_002147"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312908.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFP9897599.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            20667..21008
                     /gene="frwD"
                     /locus_tag="ACHOUK_002148"
     CDS             20667..21008
                     /gene="frwD"
                     /locus_tag="ACHOUK_002148"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFP9897600.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCLLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQQVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(20995..21846)
                     /gene="yijO"
                     /locus_tag="ACHOUK_002149"
     CDS             complement(20995..21846)
                     /gene="yijO"
                     /locus_tag="ACHOUK_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9897601.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(22061..23794)
                     /gene="eptC"
                     /locus_tag="ACHOUK_002150"
     CDS             complement(22061..23794)
                     /gene="eptC"
                     /locus_tag="ACHOUK_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFP9897602.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNMFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELNALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKENTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(23977..26628)
                     /gene="ppc"
                     /locus_tag="ACHOUK_002151"
     CDS             complement(23977..26628)
                     /gene="ppc"
                     /locus_tag="ACHOUK_002151"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFP9897603.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKTLWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(26980..28131)
                     /gene="argE"
                     /locus_tag="ACHOUK_002152"
     CDS             complement(26980..28131)
                     /gene="argE"
                     /locus_tag="ACHOUK_002152"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFP9897604.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASTGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICAWCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            28285..29289
                     /gene="argC"
                     /locus_tag="ACHOUK_002153"
     CDS             28285..29289
                     /gene="argC"
                     /locus_tag="ACHOUK_002153"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFP9897605.1"
                     /translation="MLNTLIVGASGYAGAELVTCVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLDAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            29297..30073
                     /gene="argB"
                     /locus_tag="ACHOUK_002154"
     CDS             29297..30073
                     /gene="argB"
                     /locus_tag="ACHOUK_002154"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFP9897606.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            30134..31507
                     /gene="argH"
                     /locus_tag="ACHOUK_002155"
     CDS             30134..31507
                     /gene="argH"
                     /locus_tag="ACHOUK_002155"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFP9897607.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEELPLTELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FARARLG"
     gene            31774..32691
                     /gene="oxyR"
                     /locus_tag="ACHOUK_002156"
     CDS             31774..32691
                     /gene="oxyR"
                     /locus_tag="ACHOUK_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFP9897608.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(32674..34074)
                     /gene="sthA"
                     /locus_tag="ACHOUK_002157"
     CDS             complement(32674..34074)
                     /gene="sthA"
                     /locus_tag="ACHOUK_002157"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFP9897609.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
BASE COUNT         7765 a         8658 c         9568 g         8328 t
ORIGIN      
        1 gtcatttgag aaaaaagagc agaatgaggt aaaaatcttt ggctgtgtat tcagtctggt
       61 aatcatccaa ggaaagctca tcagaagggt gatgactcca attagtagaa catatatttt
      121 cgataaaagc accgatgact ttttcatgaa gttctacaga actcgagaaa cagatggttc
      181 tgcgggccag acgtttgatt cgggttctca gggtcagatt gttacgctca atgtgctgag
      241 taaaaatctt accagtcaga tgcttctcct ttggtaactc tctagcgtaa ctaccccatt
      301 cgtcagtggt caacatgccg atggtaaaag ggtaagcagc gccagcaatt cacgacaggt
      361 ttcatcagtc cgggggccaa aagtgtaggc cagaacacca ccagttttcg tgttgtacac
      421 gtaccagagc cagtgctgtc gcgctttact gccgacaaag ctccattgct gactaagttt
      481 gcagatgagt gccacatcag catgagcgac cggggatgac gttattcttc gtggcgagag
      541 ttttttaaag ttcggatgac tgtattaatg ccgattttca gcgtacgggc agtatagcga
      601 accccggcac cgttgaaagc catttcagcg atttggtctt taactcccgg tttacggggc
      661 tcgtaacagt aactgagctg aaatacccgg tgacagtcac ggccgcggaa tcgatcatgt
      721 cttttggggt tctgcccgcg acggtaaacc tgaacggact ggcaacgggg acaatgaagg
      781 ttaactgtga ccatgaaaca tccttaaaag cgagtattat atccttaatt caactaattg
      841 gaggcatcac cgtttaccgg agcgacagct tgcggaaagg tcgtgcaact acaaagcaac
      901 aaacagacta cggcgggaat tatttttttc atcatccatt accacgaaaa cagcggccat
      961 tctttggcaa ccgtggtgag ttgtggcgca tcgaaaacgg cttgtggtga gaccaccgtt
     1021 tcctcaagcg gaatattttt gccgtagcgt tctttgagct tcgcctggac cagcgggttg
     1081 ctgagatcaa actctttcaa cttctgctgt tcgcccacac gcattcccag cgcattctga
     1141 taaaccttcc acaccacttc cgaacagtac tggcgatcgt cgctccatga gaagctgaaa
     1201 tcgtacggtt tgccgagata acgtttcgcc gtttgcgcca gtttttgctg ttgttcaaca
     1261 ctcagtccgc cttcaacgcg gcgaacaacg tatttgccct tttcaccatg ggcaatccac
     1321 tgcttgagcg gggtgtattt caccgggccg accgcttcaa aaacgtaggg ctttttgtta
     1381 cgcatcacca gcataccggt gtggctgtaa tcggaatggg tcgccagttg gatcgcttta
     1441 ctttgcgatg agcgagagat ttgaaagatg atgtcgccag tttgcggctg ccaggcaaag
     1501 gcaggcacag aaaccagcag gctgaggatc agtagcctga ttttcatcgt taaacgtcct
     1561 tgtatgaaaa aagcggctgg ttaactccgc gccgctcttt tccgctttag tattcccacg
     1621 tctccgggtt aatccccatc tcacgcatga tctcttttgc cgcttccggg atttcgtcgc
     1681 tgcgctcttt acgcagatcg gcatcatccg gcaaaggttg cccggtaaag gcatgcagaa
     1741 acgcttcgca cagcagctcg ctgttggtag cgtgacgcag gttgttcacc tgacgacgcg
     1801 tgcgttcatc ggtgaggatt tttaacacct taagaggaat ggaaaccgta atctttttga
     1861 cttgttcact cttcttgccg tgctcagcgt atgggctgat atattcgccg ctccattcag
     1921 ccatgagata cttaatcctc ttcgtcaata aattgagacc agaccacaca atttatgtgg
     1981 gtactgaccg taaacccgca tagtttaccg tacaggcgtt accgtgacat cgtgtaatgc
     2041 acctgtcggc gtgataatgc atataatttt aacggctatt tgggatttgc tcaatctata
     2101 cgcaaagaag tttagatgtc cagatgtatt gacgtccatt aacacagtgt ttactctggt
     2161 gcctgacatt tcaccgacca agcccaggga acttcatcac atgacgcgta aacaggccac
     2221 catcgcagtg cgtagcgggt taaatgacga cgaacagtat ggttgcgttg tccctcccat
     2281 ccatctttcc agcacctata actttaccgg atttaatgaa ccgcgcgcgc atgattactc
     2341 gcgtcgcggc aacccaacgc gcgatgtggt tcagcgtgcg ctggcagaac tggaaggcgg
     2401 tgctggtgca gtacttacta ataccggcat gtccgcgatt catctggtaa cgaccgtctt
     2461 tttgaaacct ggcgatctgc tggttgcgcc gcacgactgc tacggcggta gctatcgcct
     2521 gttcgacagc ctggcgaaac gcggttgcta tcgcgtgttg tttgttgatc aaggcgatga
     2581 acaggcatta cgggcagcgc tggcagaaaa ccccaaactg gtactggtag aaagcccaag
     2641 taatccattg ttacgcgtcg tggatattgc gaaaatctgc catctggcaa gggaagtcgg
     2701 ggcggtgagc gtggtggata acaccttctt aagcccggca ttacaaaatc cgctggcatt
     2761 aggtgccgat ctggtgttgc attcatgtac gaaatatctg aacggtcact ctgacgtggt
     2821 ggctggcgtg gtgattgcta aagacccgga cgttgtcact gaacttgcct ggtgggcaaa
     2881 caatattggc gtgacgggcg gcgcgtttga cagctatctg ctgctacgcg ggttgcgaac
     2941 gctggtgccg cgtatggagc tggcgcagcg caacgcgaag gcgattgtga aatatctgca
     3001 aacccagccg ttggtgaaaa aactgtatca cccgtcgctg ccggaaaatc aggggcatga
     3061 aattgccgcg cgtcagcaaa aaggctttgg cgcaatgttg agttttgaac tggatggcga
     3121 tgagcagacg ctgcgccgtt tcctgggcgg gctgtcgtta tttacgctgg cggaatcatt
     3181 agggggagtg gaaagtttaa tctctcacgc cgcaaccatg acacatgcag gcatggcacc
     3241 agaagcgcgt gctgccgccg ggatctccga gacgctgctg cgtatctcca ccggtattga
     3301 agatggcgaa gatttaattg ccgacctgga aaatggcttc cgggctgcaa acaaggggta
     3361 aaaatgagtg tgattgcgca ggcaggggcg aaaggtcgtc agctgcataa atttggtggc
     3421 agtagtctgg ctgatgtgaa gtgttatttg cgtgtcgcgg gcattatggc ggagtactct
     3481 cagcctgacg atatgatggt ggtttccgcc gctggtagca ccactaacca gttgattaac
     3541 tggttgaaac taagccagac cgatcgtctc tctgcgcatc aggttcaaca aacgctgcgt
     3601 cgctatcagt gcgatctgat tagcggtctg ctacccgccg aagaagccga tagcctcatt
     3661 agcgcttttg tcagcgatct tgagcgcctg gcggcgctgc tcgacagcgg tattaacgac
     3721 gcagtgtatg cggaagtggt gggccacggc gaagtgtggt cggcgcgcct gatgtctgcg
     3781 gtgcttaatc aacaagggct gccagcggcc tggcttgatg cccgcgagtt tttacgcgct
     3841 gaacgcgctg cgcaaccgca ggttgatgaa gggctttctt acccgttgct gcaacagcta
     3901 ctggtacaac atccgggtaa acgtctggtg gtgaccgggt ttatcagccg taacaacgcc
     3961 ggtgaaacgg tgctgctggg gcgtaacggt tccgactatt ccgcgacaca aatcggtgcg
     4021 ctggcgggtg tttctcgtgt aaccatctgg agcgacgtcg ccggggtata cagtgccgat
     4081 ccgcgtaaag tgaaagatgc ctgtctgctg ccattgctgc gtctggatga agccagcgaa
     4141 ctggcgcgtc tggcggctcc cgttcttcac gcccgtactt tacagccagt atccggtagc
     4201 gaaatcgacc tgcagctgcg ctgtagctac acgccggatc aaggctccac gcgcattgaa
     4261 cgcgtgctgg cctccggtac tggtgcgcgt attgtcacca gccacgatga tgtctgcttg
     4321 attgagtttc aggtgcccac cagccaggat ttcaaactgg cgcataaaga gatcgaccag
     4381 atcctgaaac gtgctcaggt gcgcccactg gcggttggcg tgcataacga tcgccagttg
     4441 ctgcaatttt gctacacctc agaagtggcc gacagtgcgc tgaaaatcct cgacgaagcg
     4501 ggattacctg gcgaactgcg cctgcgtcag gggctggcgc tggtggcgat ggtcggggcg
     4561 ggtgtcaccc gtaacccgct gcattgccac cgcttctggc agcaactgaa aggccagccg
     4621 gtcgagttta cctggcagtc cgatgacggc atcagcctgg tggcagtact gcgcaccggc
     4681 ccgaccgaaa gcctgattca gggcctgcat cagtccgtct tccgcgcaga aaaacgcatc
     4741 ggcctggtat tgttcggcaa aggcaatatc ggttcccgct ggctggaact gttcgcccgc
     4801 gagcagagca cgctttcggc gcgtaccggc tttgaatttg tgctggcagg cgtggtggac
     4861 agccgccgca gtctgttgag ttatgacggg ctggacgcca gccgcgcgtt agccttcttc
     4921 aacgatgaag cggttgagca ggatgaagag tcgttgttcc tgtggatgcg cgcccatccg
     4981 tatgatgatt tagtggtgct ggacgttacc gccagccagc agctggctga ccagtatctt
     5041 gatttcgcca gccacggttt ccacgtcatc agtgccaaca aactggcggg agcaagcgac
     5101 agcaataaat atcgccagat ccacgacgcc ttcgaaaaaa ccgggcgtca ctggctatac
     5161 aacgccaccg tcggtgcggg cttgccgatc aaccataccg tgcgcgatct gatcgacagc
     5221 ggcgatacta ttttgtcgat cagtgggatc ttctccggca cgctctcctg gctgttcctg
     5281 caattcgacg gtagcgtgcc gtttaccgag ctggtggatc aggcgtggca gcagggctta
     5341 accgagcctg atccgcgtga cgacctctcc ggcaaagacg taatgcgcaa gctggtgatt
     5401 ctggcgcgtg aagcgggtta caacattgaa ccggaccagg tacgtgtgga atcgctggtg
     5461 cctgctcatt gcgaaggcgg cagcatcgac catttctttg aaaatggcga tgaacttaac
     5521 gagcagatgg tgcaacggct ggaagcggcc cgcgaaatgg ggctggtgct gcgctacgtg
     5581 gcgcgtttcg atgccaacgg caaagcgcgt gtgggcgtgg aggcggtgcg tgaagaccat
     5641 ccgctggcat cactgctacc gtgcgataac gtctttgcca tcgaaagccg ctggtatcgc
     5701 gacaacccgc tggtgatccg tgggcctggc gcggggcgcg acgtcaccgc cggggcgatt
     5761 cagtcggata tcaaccggct ggcacagttg ttgtaatttc agaaatttaa taatgcccgg
     5821 tactcatgtt ttcgggttta tggtttctaa tgaaatatat tgaattatca taggattagg
     5881 ccggatgaag cgtttacgac gaatccggca agaagcaata agtacatggt tagtttatat
     5941 ttgcagtccg gtttgctttg cataccggat tttctttttc ttaccatcct gaagtttttt
     6001 catcttccct gatttttcct caccatcatt ggtcattttt cggttgacgc ccttcggctt
     6061 ttccttcatc tttacatctg gacgtctaaa cggatggatg tgcacaacac aacatataac
     6121 tacaagcgat tgatgaggta aggtatgaac ttttttcacg ccagccagcg ggatgccctg
     6181 aatcagagcc tggcagaagt ccaggggcag attaacgttt cgttcgagtt tttcccgccg
     6241 cgtaccagtg aaatggagca gaccctgtgg aactccatcg atcgccttag cagcctgaaa
     6301 ccgaagtttg tatcggtgac ctatggcgcg aactccggcg agcgcgaccg tacgcacagc
     6361 attattaaag gcattaaaga tcgcactggt ctggaagcgg caccgcatct tacttgcatt
     6421 gatgcgacgc ccgacgagtt gcgcaccatt gcacgcgact actggaataa cggtattcgt
     6481 catatcgtgg cgctgcgtgg cgatctgccg ccgggaagtg gtaagccaga aatgtatgct
     6541 tctgacctgg tgacgctatt aaaagaagtg gcagatttcg atatctccgt ggcggcgtat
     6601 ccggaagttc acccggaagc aaaaagcgcc caggcggatt tgcttaacct gaaacgcaaa
     6661 gtcgatgcag gagccaaccg cgcgattact cagttcttct ttgatgtcga aagctacctg
     6721 cgttttcgtg accgctgtgt gtcggcgggc attgatgtgg aaatcattcc gggcatattg
     6781 ccggtatcta actttaaaca ggcgaagaaa tttgccgaca tgaccaacgt gcgtattccg
     6841 gcgtggatgg cgcaaatgtt tgacgggctg gatgacgatg ccgaaacccg caagctggtg
     6901 ggcgcgaata ttgccatgga tatggtgaag attttaagcc gtgaaggagt gaaagatttc
     6961 cacttctata cgcttaaccg cgctgaaatg agttacgcga tttgccatac gctgggggtt
     7021 cgacctggtt tataaatagt gtggctttta tgaaaatcac acagtgatca caaattttaa
     7081 acagagcaca aaatgctgcc tcgaaatgag ggcgggaaaa taaggttatc agccttgttt
     7141 tctccctcat tacttgaagg atatgaagct aaaacccttt tttataaagc atttgtccga
     7201 attcggacat aatcaaaaaa gcttaattac gatcaatttg atctacatct ctttaaccaa
     7261 caatatgtaa gatctcaact atcgcatccg tggattaatt caattataac ttctctctaa
     7321 cgctgtgtat cgtaacggta acactgtaga ggggagcaca ttgatgagca cgtcagacga
     7381 tatccataac acgacagcca ctggcaaatg cccgttccat cagggcggtc acgaccagag
     7441 cgcgggggcg ggcacaacca ctcgcgactg gtggccaaat caactccgtg ttgacctgtt
     7501 aaaccaacat tctaatcgtt ctaacccgct gggtgaggac tttgactacc gcaaagaatt
     7561 cagcaaatta gattactacg gcctgaaaaa agatctgaaa gccctgctga cagaatctca
     7621 gccgtggtgg ccagccgact ggggcagcta cgccggtctg tttattcgta tggcctggca
     7681 cggcgcgggg acttaccgat caatcgatgg acgcggtggc gcgggtcgtg gtcagcaacg
     7741 ttttgcaccg ctgaactcct ggccggataa cgtaagcctc gataaagcgc gtcgcttgtt
     7801 gtggccaatc aaacagaaat atggtcagaa aatctcctgg gccgacctgt ttatcctcgc
     7861 gggtaacgtg gcgctggaaa actccggctt ccgtaccttc ggttttggtg ccggtcgtga
     7921 agacgtctgg gaaccggatc tggatgttaa ctggggtgat gaaaaagcct ggctgactca
     7981 ccgtcatccg gaagcgctgg cgaaagcacc gctgggtgca accgagatgg gtctgattta
     8041 cgttaacccg gaaggcccgg atcacagcgg cgaaccgctt tctgcggcag cagctatccg
     8101 cgcgaccttc ggcaacatgg gtatgaacga cgaagaaacc gtggcgctga ttgcgggtgg
     8161 tcatacgctg ggtaaaaccc acggtgctgg tccgacatca aatgtaggtc ctgatccaga
     8221 agctgcgccg attgaagaac aaggtttagg ttgggcgagc acttacggca gcggcgttgg
     8281 cgcagatgcc attacctctg gtctggaagt ggtctggacc cagacgccga cccagtggag
     8341 caactatttc ttcgagaacc tgttcaagta tgagtgggta cagacccgca gtccggctgg
     8401 cgcaatccag ttcgaagcgg tagacgcacc ggaaattatc ccggatccgt tcgatccgtc
     8461 gaagaaacgt aaaccgacga tgctggtgac cgacctgacg ctgcgttttg atcctgagtt
     8521 cgagaagatc tctcgtcgtt tcctcaacga tccgcaggcg ttcaacgaag cctttgcccg
     8581 tgcctggttc aaactgacgc acagggatat ggggccgaaa tctcgttaca tcgggccgga
     8641 agtaccgaaa gaagatctga tctggcaaga tccgctgccg cagccgatct acaacccgac
     8701 cgagcaggac attatcgatc tgaaattcgc gattgcggat tctggtctgt ctgttagtga
     8761 gctggtatcg gtggcctggg cttctgcttc taccttccgt ggtggcgaca aacgcggtgg
     8821 tgccaacggt gcgcgtctgg cattaatgcc gcagcgcgac tgggatgtga acgccgcagc
     8881 cgttcgtgct ctgcctgttc tggagaaaat tcagaaagag tctggtaaag cctcgctggc
     8941 ggatatcatc gtgctggcag gtgtggttgg cgttgagaaa gccgcaagcg ccgctggttt
     9001 gagcattcat gtaccgtttg cgccgggtcg cgttgatgcg cgtcaggatc agactgacat
     9061 tgagatgttc gaactgctgg agccaattgc tgacggtttc cgtaactatc gcgctcgtct
     9121 ggatgtttcc accaccgagt cactgctgat tgataaagcc cagcaactga ccctgaccgc
     9181 gccggaaatg accgcgctgg tgggcgggat gcgtgtactg ggtgccaact tcgatggcag
     9241 caaaaacggc gtcttcactg accgcgttgg cgtattgagc aatgacttct tcgtgaactt
     9301 gctggatatg cgttacgagt ggaaagcgac cgacgaatcg aaagagctgt tcgaaggccg
     9361 tgaccgcgaa accggcgaag tgaaatacac tgccagccgt gcggatctgg tatttggttc
     9421 taactctgtc ctgcgtgcgg tggctgaagt ttacgccagc agcgatgctc acgagaagtt
     9481 tgttaaagac ttcgtggcgg catgggtgaa agtgatgaac ctcgaccgtt tcgacctgct
     9541 gtaatctgac cttcttcagc gactgccttt caggcagtcg ctgaagtttc tttaccggcg
     9601 tatagtgtcc acaggaaaac tacacactgg atctctcatg tctgccgcag gaaagagcaa
     9661 cccactggca atcagtggcc tggttgtgct cacacttatc tggagttata gctggatttt
     9721 catgaagcaa gtcaccagct acatcggtgc cttcgacttt accgccttac gctgcatttt
     9781 cggcgctctc gttttattca tcgtcctttt attacgtggt cgcggaatgc gcccgacacc
     9841 gtttaaatac accttagcca ttgccctgtt acaaacctgc gggatggttg gcctggcgca
     9901 gtgggcgctg gtcagcggcg gagcggggaa ggtggcgatc ctgagctata ccatgccgtt
     9961 ctgggtggtg attttcgccg cggtgtttct cggtgaacgc ctgcgacgtg ggcaatattt
    10021 cgcgattctg attgccgctt tcggcttatt tttggtgttg cagccgtggc aactcgattt
    10081 ctcttcgatg aaaagtgcca tgctggcaat cctctccggt gttagttggg gggcgagcgc
    10141 gattgttgct aaacgtctgt atgcccgtca tccgcgcgtg gatttattgt cgttaacatc
    10201 ctggcagatg ctctatgcgg cgctggtgat gagtgtggtc gctttactgg tgccgcaacg
    10261 tgaaattgac tggcagccca ctgtgttctg ggctctggcc tacagtgcga ttctggcgac
    10321 ggcgttggcg tggagcttat ggctgtttgt gctgaaaaac ttgcctgcca gtattgccag
    10381 cttaagcaca ctggccgttc ccgtttgcgg cgtactcttt tcctggtggc tgctcggtga
    10441 gaatccgggg gccgttgaag gtagcggtat tgtgctgatt gtgctggcac tggcgctggt
    10501 gagccgtaag aaaaaagaag ccgttagtgt aaaaaggatc tgaatttttt cttcatgtgg
    10561 ggcgatcgct tatttaacaa aataacgata atgccccacc atgcgccagc taaacagcac
    10621 atcttcttcc tgcgcacctg cgccaatgtt atgtatcacc agcggcgtac cgtcgcgggc
    10681 gaagccatct gaaaccaccc caatatgtgc cagcccgtta tctaatcgcc aggagacaat
    10741 atcgcccgct tgatagtcac tggggttctt gctgatgggg cgtgttttat cgtggcgggt
    10801 aaaccaggtt tccagattag gcacccgacg gtgatcgatg ttgctgtccg ggcgctttaa
    10861 ctgccatttt tgcgggtact cggcaaaatt cttcgccata tcttcgtgca ccagtttctg
    10921 caaatcgacc ttctggctgc gcaatgcacg gatcaccaca tcggaacata ccccgcgttc
    10981 ttgcgggaca tcaccgccag gataggtaag ctgcacatac gccggatcgt aaaatagcgt
    11041 gctgccgatt tgctgtctgg caccgtctgc gatggcaagg ttggtattgg cctggatttg
    11101 taccacggtt ggtggaatgg cgggagattt taaggagtag ctggtaaatg ccgttagcag
    11161 gctgaccagc gccagtgaag ctttcatctg tttcgtccgt gaaaataaga gtcacagatt
    11221 cgaccttccg ggctaacgat tgttctgtag agaaaatgtc cagaagcgga taaaaaattc
    11281 ctgaacaacg tgggagtttc agattactgg cacagagtgc cggatgcggc gtgaacgtct
    11341 tatctggcct aaaaagcacg caaattcaat atattgcaga gattgcgtag gcctgataag
    11401 cgtagcgcat caggcaattt tgcgttcaaa ctcccggaca agccgggagt ttggagtagg
    11461 ttattcccac tcttgcagga aacgctgacc gtactggtcg gctaccagca gagcggcgta
    11521 aacctgatct ggcgtcgcgc cgccaggcat gttgtggatg gtttcacctt ctgcacatgc
    11581 cgcttctgcc acaattcgca ttttcgccgg gacatcttct ttaatatcca gttgagcgag
    11641 agttattggc aaacctaccg catggctaag cgcagctacg gtttcgattt cctcaaccgg
    11701 cgagttttcc agtaccagct gcgtcagtgt accaaatgcc actttttcac cgtgataata
    11761 gtgatgggca tcaggaatgg cagtcaggcc attatgcacc gcgtgcgctg ccgccagacc
    11821 accgctttca aagccaacac cgcttaaata agtgttcgct tcaatcacgc gttccagtgc
    11881 cggagtcact acatgctgtt cggcggcgag catcgctttt tcgccttctt ccagcaaggt
    11941 gttgtagcac agttcagcca gcgccagcgc cgcctgagtg cacttgccgc ccgccatggt
    12001 ggtcgcaccg ctacgagagc aggcacgcgc ttcaaaccag gtcgccagcg catcgccgat
    12061 acccgccgct aacagacgcg caggtgcgcc cgcgacgatt ttagtatcga caatcaccat
    12121 attcgggtta tttggcagca gcagatagcg gtcaaattca ccttcgtcag tgtagataac
    12181 ggaaagagcg ctacaaggtg cgtcggttga agcaatggtc ggggcaatgg cgaccggaac
    12241 acccatgaaa tgtgccagtg ctttggctgt atcgagggtt tttccgccac cgataccgag
    12301 aattgcgcca cactgcgcag tctccgcgat gccacgcaga cggtcgatct cattttgcga
    12361 acattcaccg ccaaacggcg caatctctac taccagtcca gcatctttaa agcttttctc
    12421 gacagtggat tgagcaaaac ctaaaacaaa tttgtcaccc accactaacc agcgttctgc
    12481 cagcggcttc aggtattcgc ccagacgatt aatcacatca gcgccctgga tgtatttacc
    12541 cggtgattga ataatgcggt ccataattgc tcctttagag atgagtagtg ccaaatgcgg
    12601 cattccagtc gtgttcgaac ttctctatag ctgactctac cgcaggggtg ttgagcattt
    12661 gttgcgctac atctaagggc agggtgatgg attcacatcc tgccagtaaa cagtccagcg
    12721 cctgacgcgg cgttttaaag ctggccgcca gcaccatgct ttctggtgcg tgcatttcta
    12781 acagcgcttg cagctcctga accgtacgaa tgccgtcccc gccctgggca tccacgcggt
    12841 taacatacgg agcaacgtat tttgcccctg ccagtgcggc taataaccct tgtgcggcgc
    12901 tatatacagc agtgccaagt gtagtaatac cctctttttt cagtatttta attgctgcca
    12961 gaccttcgga agtcaccggg attttcacca caatgcccgg aatggcatcg cgcagacgct
    13021 tcgcttcttc caccatcccc tgcgcgtcgc ggctcatggt ctgagcaaac agaatgccct
    13081 catcaccaat cgctttttgc agacgcggca gcacttccca tatggactcc ttgctggcag
    13141 cgataatgct cgggttagtt gtcaccccgg caatcgggaa tatgtgtgcc agacgttcga
    13201 cttctgcgac gttagcggtg tccagataca gttccatgat gtttcctttt acagttccag
    13261 ttcatgttgc agcaggctgg cgatagcgtc ggcagaggcg gcgttaacca gcgcgttacg
    13321 gaattcttcg tgcatgatgc ggcgagcgag gcgcgagaaa atgcgcatat gctgatcgcc
    13381 cgcagcgtgt ttgttcaggg ttaacataat gatgaattgc gcttcatcat cgccccagcg
    13441 caccggcgct tgcagacgcg ctacgctgat ggtggattgc tcaatgtgtt ctgatttgct
    13501 gtggggaatg gcaaaactaa agcccagacc ggtagagaaa acggcctcgc gcgcccacaa
    13561 gtcggcttcc agtttacgcg gatagcggca gcggcccgcc agcagcaggt tatcggtcat
    13621 gcctttgagc acttcttctt tgctgcgcca gtcgctttcc agtgtgatgc actcggcggt
    13681 gaccagcggt gcgtcttgtt gggtcatgcg gaattgcgcc agcaggtgtt ccacttccag
    13741 cgaagtacgg caggccattg cctggttgag caacttgcgg cactcacggc tatcaagttg
    13801 cgccatccgc gctttcgccg ccggaattga tggtgcgctc atgctgagtt catccagccc
    13861 taagccgacc agcaacggca gcacggaacc tttcgctccc agctcaccgc acagaccaat
    13921 ccatttgccc tggcgatgca ccgcttgcac ggcgtaatcg agcgcccgca agaatgccgg
    13981 attcaggctg ttgtagtgac gagtaacctt agcgttatcg cgatccactg ccagcagata
    14041 ttgcgtcagg tcattactac caatactaaa gaaatcaatc tcttcgcagc attgatcgat
    14101 gatgaacatc accgacggca cttccagcat gatgccgagc tggatcttct catcaaacgg
    14161 aatgtgttcg ttacgcagtt gctgtttggc ttccgccagt ttttctttca cccataagat
    14221 ctcttccatt gaggagatca tcgggatcat gattttcagg ctgccgtgag cagaggcacg
    14281 gaggatcgac cgtagttgtg tggtgaacag cgaggcgtat tcttcataaa tacgcaccgc
    14341 gcgataaccg aggaacgggt tagcttctgc cggaatgttc agataatcaa cgggtttgtc
    14401 accgccaata tccatagtgc gcacaataat gctgcgtccg ttggcggact ccagcgcttg
    14461 acaaaaaatg ttgtacaact cgctttcgcc cggtgcgctg gtgcgatcca tatagagcat
    14521 ttcagtgcgg aacaaaccga cgccttccgc accattgccg aatgcggcct gcgcttccac
    14581 ggagtgagcg atgttagcgg caatttcaat gcggataccg tcagcggtac gggcttgttg
    14641 ggtcagccag acacgctgtt gctcacgcag ggcgtcctgt acgcgggctt cttgctgata
    14701 ataacgagct acggcttccc ctggctcaac cacaatcgcc ccggcgttgc cgtcgatata
    14761 aatcgtttga tgctgccacg gagtaagggc atcaatatct acaccaacca gtgtcggaat
    14821 gttgaacgaa cgggcaagaa ttacggtatg tgaggtggtg ccgccacttt tcagtaacag
    14881 acctttgagg tgatttttat ccagttcgag gaactggctg ggggttagtt catcagccat
    14941 acaaatggcg ggctgcgtca gtttgcccgg tgccgggaag cgttgctcac cgtagatttg
    15001 ctgaagcagc tggaagcaga catcgcgtac gtccagggca cgttcttgca ggtagctgct
    15061 gctggaacgg gaaaactctt cacaaaagtg attcgcactg gcgacaatcg cttcggcgca
    15121 gcttaatccg gcgctgacgc ctgccagtaa atgttcgtgc agggaagtat cgccagccag
    15181 ggatcggtgc gcttccagaa tcgcgctggt ggcaccgtcg ctatccagca gacgaaactc
    15241 aatgtttttc agtaccagcg tcaggccgtt ttccagtgcg gattgctcgg cgtcaacgtc
    15301 tttggctgcg ggaagattac ccagcgcatt gagatctaaa gaagagatcg gcgtcagaat
    15361 gccgcctgcg ctaccgctgc acacggtgcg ggcgcggata atttgcggat tcagattggt
    15421 cagtgaaacc ggcagtggtt ccagttcgtc agatttaact tccgccagcg gcgcatcgca
    15481 gtgggggaat tcatcgcgca gccattggct taaacgctgg tgcgcttcct gttcgtcggc
    15541 cccggaaatc agtaactggc agttatcgcc cgccagcgtg tcggtgccaa tcagcgcaag
    15601 ggcgcttttg gcgttgccct tgcggtcagt gcgcaggtta tgccactcaa tttgtgatga
    15661 aaaagtatta cacagcgttt caacgtggct tgccggacgt gcatgtacgc cgttaggtag
    15721 ctcacaaata aattccacaa tcagggccat tgcctctctc ccggtacggt tttctgttta
    15781 caggataaga gcgcacggca acggcctgcc atgtgacaaa tctgccaaaa gctggacaaa
    15841 tgtaatgtaa ccgtcaattt gcgacgcgtc tcacaagacg ccgttttgcg gcatgcttcc
    15901 ggattatcgc aagttatgag gcggatcgca tttttgtact gatattacaa aagtccagta
    15961 aatggccttt ttatccactg ttttctccgc ctgcgattgc ctattgtttc ctccatcaaa
    16021 aatatggaca tgggctttcg caagcccggg agcagcttat gaatgagttg gtgcagatcc
    16081 tgaaaaatac ccgtcagcat ttaatgacgg gcgtttcaca catgattccc ttcgtggtat
    16141 cgggcggtat tttgctggcg gtttccgtca tgttgtatgg caaaggcgca gtgccggatg
    16201 ccgtagccga tccgaatctg aaaaaactgt ttgatatcgg cgttgcaggt ttgacgctga
    16261 tggtgccttt cctcgccgct tacatcggtt actccattgc agagcgttct gcgctggctc
    16321 cgtgcgctat cggtgcctgg gttggtaaca gctttggtgc gggcttcttt ggtgcactga
    16381 tcgccgggat tatcggcggc atcgtggtgc attacctgaa gaaaattccg gtgcataaag
    16441 ttctgcgctc ggtgatgccc atcttcatca ttccgatcgt cggcacactg attaccgcag
    16501 gcatcatgat gtggggtctg ggcgagcctg taggggcgtt gaccaacagc ctgactcagt
    16561 ggcttcaggg gatgcagcag ggcagcattg ttatgctggc ggtgatcatg ggtctgatgc
    16621 tggcgttcga tatgggcggt ccggttaaca aagtggccta tgccttcatg ctgatttgcg
    16681 ttgctcaggg tgtttatacc gtggtggcca ttgctgccgt gggtatttgt atcccgccgc
    16741 tggggatggg gctggcgacg ctgattggtc gtaaaaattt ctccgcagaa gagcgcgaaa
    16801 ccggtaaagc ggcgctggtg atggggtgcg ttggggttac tgaaggggcg attcctttcg
    16861 ccgcagccga tccgttacgt gtaattcctt ccatcatggt tggttcagtt tgtggtgcag
    16921 taactgcggc gctggtcggt gcgcagtgct atgcaggctg gggtggtctg attgtgctgc
    16981 cggtggttga aggtaagttg ggttatatcg cagcagtggc tgtcggggcg gttgtgacgg
    17041 cagtctgcgt gaacgtgctg aaaagtctgg cgcgtaaaaa tggatcttct actgatgaaa
    17101 aagaagacga cctggatttg gattttgaaa ttaattaatt gaggaacgag ccatgacgaa
    17161 aattattgca gtaaccgcgt gtccatctgg tgttgcccat acttatatgg ctgcagaggc
    17221 gctggaaagt gccgcgaaag cgaaaggctg ggaagtgaaa gtagaaaccc agggctcgat
    17281 tggtctggaa aacgaactga ctgcggaaga cgtggcgagc gccgatatgg tgattctgac
    17341 taaagatatc ggcatcaagt ttgaagagcg ttttgctggc aagaccatcg tgcgcgtcaa
    17401 catcagcgat gcggtaaaac gtgctgatgc cattatgagc aaaattgaag ctcacctggc
    17461 gcaaaccgct taagttcttc tcccgctcgc aagggcgggt tcgctttccc acaggagttc
    17521 ctcatgacga atcgtatctc tcgcctcaaa actgcactgt ttgccaatac ccgtgaaatc
    17581 tcgctggagc gtgcgctgct ttataccgcc agccatcggc aaaccgaagg cgaaccggtg
    17641 atattgcgcc gggcgaaagc aacagcgtat atccttgaac atgttgaaat ttcgattcgt
    17701 gatgaagaac tgattgccgg taaccgcacc gtaaaaccgc gcgccgggat tatgtcgccg
    17761 gaaatggacc cttactggct gctgaaagag ctgggtcaat tcccgacgcg tccgcaggac
    17821 cgttttgcta tcagcgaaga agataaacgt atctaccgcg aagagttgtt cccgtactgg
    17881 gaaaaacgtt cgatgaaaga tttcatcaac gggcagatga cggatgaagt aaaagccgcg
    17941 actagcacgc agattttcag catcaaccag acggataaag gccaggggca cattattatt
    18001 gattacccgc gcctgctgaa tcacgggctg ggggagctgg tggcacagat gcagcaacat
    18061 tgtcagcaac agccggagaa tcacttttat caggcagcgc tgttactgct cgaagcctcg
    18121 cagaaacaca ttttgcgtta cgccgaactg gcggaaacga tggcggcaag ctgcactgat
    18181 gcccagcgtc gcgaagaact gttgactatt gcggagatct cccgccataa cgccgaacat
    18241 aaaccgcaga cgttctggca ggcgtgccag ttattctggt acatgaacat cattctgcaa
    18301 tacgaatcca acgccagttc gctgtcttta gggcgcttcg accagtatat gttgccgttc
    18361 tatcaggcat ctttaaccca gggcgaagat ccggcgttcc tgaaagaact gctcgaatct
    18421 ttatgggtga aatgcaacga cattgtgctg ttgcgctcca ccagcagcgc gcgttatttc
    18481 gcaggtttcc cgactggcta taccgcactg ctcggcgggt taaccgagaa cggacgtagc
    18541 gcggtgaacg tgctttcgtt cctctgcctc gatgcctatc aaagcgtaca attaccgcaa
    18601 ccgaacctag gggtgcgcac taacgccttg atcgacacgc cgttcctgat gaaaaccgca
    18661 gaaaccattc gcctcggcac cggtattccg caaatcttta acgatgaagt ggtggtgcca
    18721 gcgttcctca accgtggcgt ttcgctggaa gatgcgcgcg actattccgt agtgggctgt
    18781 gtggaattat ctattcccgg cagaacctac ggcttgcatg acatcgcgat gtttaatctg
    18841 ctgaaagtga tggaaatctg cctgcatgaa aatgaaggca atgctgcgct gacttatgaa
    18901 ggtttactgg agcagatccg cgccaagatc agccactaca tcaccctgat ggttgagggc
    18961 agcaatattt gtgatatcgg ccatcgcgac tgggcacctg taccgctgct ctcgtctttt
    19021 atcagcgatt gtctggaaaa aggtcgcgat attaccgatg gcggcgcgcg ttataacttc
    19081 tccggcgtac agggaattgg tatcgccaac ctgagcgatt ctctccatgc gttgaaaggg
    19141 atggtttttg atcaacagcg tttaagtttt gacgaattgc tgtcggtatt aaaagccaac
    19201 tttgcaacgc cagaaggcga aaaagtccgc gctcgcttaa ttaaccgctt tgagaaatac
    19261 ggtaacgata tcgacgaggt gggtaacatt agcgccgaac tgttgcgcca ctactgcaaa
    19321 gaagtggaaa aataccagaa cccgcgcggc ggttacttca cgccgggatc gtataccgtt
    19381 tctgctcacg tcccgttggg atcggtggtt ggcgcgacgc cagacggtcg ttttgccgga
    19441 gaacagttgg cagacggcgg cttgtcaccc atgctgggcc aggacgcaca agggccaaca
    19501 gcggtactga agtcagtcag taagctcgat aacacgctgc tgtctaacgg tacgttgctg
    19561 aacgtgaaat tcactccggc gaccctggaa ggtgaagcgg gattacgcaa actggccgac
    19621 ttcttacggg cgtttaccca gcttaagttg cagcatattc agtttaacgt ggtgaacgcc
    19681 gacacgttgc gggaagcgca acagcgccca caagattatg ccgggctggt ggtgcgcgtt
    19741 gccggataca gcgccttctt tgtcgaactg tcgaaggaga tccaggatga catcatccgc
    19801 cggacagcgc atcagctgta acgttgtgga aacgcgccgc aatgatgtgg cgcgcatttt
    19861 caacattcag cgttattcac tgaatgacgg cgagggcatt cgtacggtgg tcttttttaa
    19921 aggctgtccg catctttgcc cgtggtgtgc taatccggag tcgatctccg gcaaaatcca
    19981 gacggtacgc agagaggcga aatgtctgca ctgtgcgaaa tgtttgcgtg atgcggatga
    20041 atgcccctcc ggggcgtttg aacggattgg tcgcgatatc agccttgacg cgctggaacg
    20101 ggaagtgatg aaagatgaca ttttctttcg cacgtccggc ggcggcgtca cgctttctgg
    20161 cggcgaagtg ttaatgcagg cggagtttgc tacccgtttt ttacagcggc tgcggttgtg
    20221 gggcgtgtca tgcgccattg aaactgccgg agacgcgcca gccagcaagc tgttaccgct
    20281 ggcgaaattg tgcgatgaag tgttgttcga tttaaaaatt atggacgcga ctcaggcgcg
    20341 ggatgtggtg aagatgaacc tgccacgcgt gctggagaat ctgcgtttgc tggtgagtga
    20401 gggcgtcaac gtgatcccgc gtttaccgct gatccctggt ttcacgctca gccgggagaa
    20461 tatgcagcag gcgctggatg tactgatccc gctgaaaatc aggcagatcc atctgttacc
    20521 gtttcatcag tacggcgaac cgaaataccg cctgctgggg aaaacatggt cgatgaaaga
    20581 ggtgcctgcg ccgtcgtcag ccgatgtgac aacgatgcgc gaaatggcag aacgggccgg
    20641 atttcaggtt accgtgggag gttaaaatgg catacctggt ggcagtaacc gcctgcgtaa
    20701 gcggcgtggc gcatacatat atggcggcgg aacggctgga aaagttgtgc ctgttagaga
    20761 agtggggagt cagcattgaa actcagggcg cgctgggaac ggagaatcgt ttagcggacg
    20821 aggatatccg tcgggcggat gttgctctgt tgattacgga tatcgagctt gccggtgccg
    20881 agcgatttga acattgccgc tatgtgcaat gcagcatcta cgcattcctg cgtgagccgc
    20941 agcaggtaat gagcgcggtg cgcaaagtgc tttctgcgcc gcagcaaacc catcttattc
    21001 tggagtagtc ggtttttctg tcagttggct gtggtactgc cggcgatatt ccgacggcga
    21061 gcgttcggtg tttttacgaa acagacggca gaagtagttg ctgtcgacaa agccgcaggc
    21121 gtgcgccact tcttttacct tcaggtcata gcctttcaat aacgtcttag cgtgctccag
    21181 tcgcgtgtga ttcaggtatt cgttaaaacc aatggccccc gttttttgaa acaggtgcga
    21241 gagatagttt ggtgaaatat agaatgcctg tgcgacagat tcgcgggtaa gcgcggaggc
    21301 atagcgttcg tcgatataat cgcgaatagc ttcgaatagt gcctggctgc gtgaggcggt
    21361 ctggatttgg ctgccaagca aatcgcggca gtggctaagc aggctggcga cgataagcct
    21421 tgccgtttgc tgctcctgcg gctgcatttg catttcattg agcgtttgta gcagaaaaga
    21481 acctatgcgt gggccgcgtc gggcgacgtg ttgcttcgcc agattttgat attgtttgcc
    21541 atcccattgc acgacgctga agccgagttg ctgtttgcca aacagcacgc taaaggtagt
    21601 agcaggggct tgccattgtg gaaaattcca gccaccagcg gcaacgtaca gcacatcgcc
    21661 gggaactaac gttgctccag cgccggtatc aacaaactca ccttccagca caatttccag
    21721 ccgtggaaaa tcgacctgat acgcaagatc gggcaccggg ccgttactgc tggcaaagta
    21781 aatctggcgc agggacagcg ggccgttgat caggcgggag agcagatagc tgacgtcgtg
    21841 atacatgtta ttcccttagg tatggagacg ggaaaacagt aactggcatt attaaaaaag
    21901 gccacctgaa aagtgtggcc tgaggggagt tcgatgtaaa cattgcggca gttgtattgc
    21961 cggatgtggc gtaaacgcct tatccagtct accgccgcga tctgtaggcc ggataagacg
    22021 cgtcaagcgt cgcatccggc aaatagcgcc tggctgataa ttactgatta cctacctggt
    22081 cgccatacgg cagcgtgtcg taatcgatca gcgcgttttt cttgtacggg ttaccaatcc
    22141 agcgggtatt ttctttgaac tgcggattca ccactgaacg ggttggatcg taaccgtcgt
    22201 aagataagcc cgctaaatct gaccaggtgt ggatcagttc cgcaaggctg tatttacgat
    22261 caacatcctg cgagaaatca cgcggatgag tcgcttgcca tttttccgac gtccacagca
    22321 ggaacggaat ggtgtacata tgacgcgtcg ggttgtcctc attacgcccc tgggttttat
    22381 gaggcggcgt gtcgtaaacc tcttcaccgt ggtcagagaa gtaaaccagg aaaccgttcg
    22441 ggtctgctgc tttaaagtct ttaatcaggc tggcaaccac atgatcgtta tacaggttag
    22501 cgttgtcata atcgttatat gactccagct cttccgcgtt taatcccggc ggaacatgat
    22561 cggtattgcc atcaaacttg ccctgatttt ccgggtagcg gtatttgtat ttgatatgcg
    22621 tacccagcag atgaacgatg atcagtttct tcggcgcagg gtccttcagc acttcctgga
    22681 acggcttcag cacgttagtg tcgtattcac gcgcactctg cgtacgttgc tggttcatgt
    22741 agtactgctt gtcggtctgg cgcgaaaata ccgtcagcat ggtattgcgg gcggtcatcg
    22801 tctgctggtt ggtgatccag aaggttttat aacccgcctg tttcatcatg ttcatcagcg
    22861 acggctgcgt cagatacaga tccgggttct tttcattggc aaaggtcagc gcctgttgca
    22921 ggatttcaat ggtgtacgga cgagacgtaa ctacgttatt aaacacggtc agattcggat
    22981 cggttttatg cagcgcattc agctccggcg tggtttcacg cggataaccg tacagactca
    23041 tgcgtccacg ctgggttgac tcgccaatca ccagcactaa ggtgcgcggt tcgttacccg
    23101 attcatcttt gaaattagcc agcggcggca aggcattatt ttcattcagt agctttgtta
    23161 gcgagtttag ttgctgacga tactgataat agccggtcag gaattgccac ggtgcggcag
    23221 gctccatacg cgaggccagg ttatccaacg ttttctcaaa cggcttgttt ttgataaaca
    23281 tattcatggc gatcggatgc agaatcaagc cataaagcag ggcaaaagag acaacataac
    23341 gccacggctt tggaatatag accgggcgca ggcgtgtcca cagcagaact gccaccgccg
    23401 tataggccag cgcgataagc acaattttca ggctgaaata ctggcttaaa tactcgctgg
    23461 cttcgttggt gttggtttcg aacatcacaa acagaacgct ctgcgagaac tcctgaccgt
    23521 agatgacgta gtagcacagc gccgccagag aggccgccca tagcaccacg ccgatcactg
    23581 cggcaataat tttgatccgc ttcggaaaga ggaataccgg aattaaccac agcgagctga
    23641 ataacagcga gtcgcgaatg ccgttggtgc cactataacc actgatgtaa ataatggcct
    23701 gtagcagagt agagaaaaac caaaagtaga gcaatgccca acccagggct ttccagctaa
    23761 aaagaggttt agcctggact tctgtggaat gcatagtaag aacctgtctt gaaaaaaata
    23821 tcgccgaatt taccgacaat tccttaagga tatctgaagg gatatccaga atttgaataa
    23881 aatgcagaca gaaatatatt gaaaacgagg gtgttagaac agaagtattt cagaaaaccc
    23941 tcgcgcaaaa gcacgagggt ttgcaggaga ggaagattag ccggtattac gcatacctgc
    24001 cgcaatcccg gcaatagtga ccattaacgc ctgctcgacg cgaggatccg gttcctggcc
    24061 ttctttttct gcctggcggg agcggtgcag caactcggct tgcaatacgt tcagcgggtc
    24121 ggtgtaaata ttccgtagct gaatagactc tgcaatccac ggcagatcgg ccatcagatg
    24181 ggaatcgttg gcaatcgcca gcaccacttt gatgtcttct tcttgcagat tgcgtaactc
    24241 tttacctaac ggccacagtg ttttgtctac caggcgttgg tcatagtatt ccgccagcca
    24301 caggtctgct ttggcgaaga ccatctccag catgccgaga cgcgtcgaga agaatggcca
    24361 atcgcggcac atagcctcca gctcgctctg tttgccatct tcgaccactt tttgcagcgc
    24421 cgtacctgca cccagccagg cggggagcat cagacggttt tgcgtccagg cgaagatcca
    24481 cggaatggcg cgtagtgact cgacgccgcc ggttgggcga cgtttcgccg gacgtgaacc
    24541 caacggcagt ttgcccagtt cctgttccgg cgtagcggag cggaagtaag gcacaaaatc
    24601 tttgttttca cgtacgtagc cacggtagag atcgcaggag atgactgata gttcatccat
    24661 aatgcgacgc cagctctctt tcggctcagg tggtggcagc aggttggctt ccagtatcgc
    24721 cccggtgtaa agcgacaggc tgctgacggt gatttctggc agaccatatt taaagcggat
    24781 catctcgccc tgttcagtca cgcgcaggcc acctttcagg cttcctggcg gttgtgacag
    24841 cagcgccgca tgagcaggtg cgccgccgcg accaatggaa ccgccgcgac cgtggaacaa
    24901 cgtcagctca atacccgctt tttcgcaggt tttgattaat gcatcctgtg cctgatattg
    24961 cgcccaggac gctgccatca cgcccgcatc ttttgctgag tcggaatagc caatcatcac
    25021 catctgtttg ccctgaatca ggccgcgata ccagtcgata ttcagcaact gagtcatgac
    25081 atcgttggcg ttgttcaggt catcgagagt ttcaaacagc ggagcaaccg gcatcgcaaa
    25141 cccgataccc gcttctttca gcagcaggtg gacagccagc acgtcggacg gcgttttcgc
    25201 catcgagatc acgtaggcgg caatggagcc ttgcggtgct tcggcaatca cctggcaggt
    25261 atcgagcact tcgcgcgttt cggcgcttgg ttgccagttg cgcggcagaa gcggacgttt
    25321 ggagttcagt tcgcggatca ggaacgcctg tttgtcggcc tctgaccagc tttcgtagtc
    25381 gccgataccg aggtagcggg tcagctcgcc cagcgcttcg gtatgacgcg tgctctcctg
    25441 acggatatca atacggacca gcggtacgcc gaaacatttc acgcggcgca gggtgtcgag
    25501 cagatcgccg ttggcgataa tacccatgcc acacgcctga agtgactggt agcaagcgta
    25561 gagcggttcc cacagttctt cgttttgtgt cagcaggcct tctggttttg gcagttcttc
    25621 gcctttcagg cgcgcttcca gccatgcctg tgtcgccatc aggcgagaac gcaggttttt
    25681 catcagatag cgatacggtt ctgcggcacc ttcttcgcca accagcgcca gcagttcagg
    25741 ggtcgcttca accatcgaca gttcagaaac cagaacctga atatctttca ggaacagatc
    25801 ggtggctttc cagcggctga gcagcaggac gtggcgggtg atatcggcag tgacgttcgg
    25861 gttgccgtcg cggtcaccgc ccatccacga ggtaaaacgt accggaacaa attcgacggg
    25921 cagtttgtag ccgaggttct cttccagttg ttcgttcagt tcgcgcaggt aattcggtac
    25981 gccttgccac aggctgtttt ctactacggc aaagccccat ttggcttcat ctaccgggct
    26041 tggacgcagc ttacggattt catcggtatg ccatgactgg gcgatcaact ggcgcaggcg
    26101 acgcatcagc tggttgtgtt cgtagtcagc gatatctttg ttatcgagct gttttaagca
    26161 ggcgttcact tccaccattt tgtggatcag tgtacgacgg gtaatttcgg ttgggtgagc
    26221 cgtgaggacc agttccagcg acagcgattc cactgctttt ttgatggtgt cttcgctcag
    26281 ttccggctgg tttttcagtt tacgcagggt gcgggcgatc acttccgggt tgctggcagc
    26341 ttcgcctttc ggcgaaatgc tgtggtattg ctcggcggtg ttggccaggt tcaggaactg
    26401 actaaacgca cgcgcaacgg gcagcagctc gtcgttcgac aaattttgta aggtggtgag
    26461 taactcctgg cggttagcat cattgccagc gcgtgaagat ttggacaact tacggatagt
    26521 ttctacgcgt tcaagaatgt gttctcccaa cgcatccttg atggtttctc ccagcacttt
    26581 gccgagcata ctgacattac tacgcaatgc ggaatattgt tcgttcatat taccccagac
    26641 accccatctt atcgtttgat agccctgtat ccttcacgtc gcattggcgc gaatatgctc
    26701 gggctttgct tttcgtcgtc ttttataaag ccacgtaaaa gcggtgacgt caaatgctgc
    26761 gaaatcgctt cagcaaacga ataaatagca ggaatttacg tcattaaatt cacgacgctt
    26821 taaataagcg taacttatgg gaatgttaaa aaatcgcctc aagtaacacc aaaggtgtag
    26881 gtcggataag atgtgcaagc atcgcatccg acattattgc ggcactggag tttggcaaca
    26941 gtgccggatg cggcgcaagc gccttattcg gcctacgttt taatgccagc aaaaatggtg
    27001 aatgacctgg gttatcagtt cgcgggtggg cttgataaac cgtgtttcca gatattcatc
    27061 aggctgatga gcctgattaa ttgagccagg ccccagaacc agcgtcgggc ataacgtttg
    27121 aataaacggc gcttcggtac agtaattcac cacttcggtt tttgctccga gcaatttctc
    27181 aaccacttca accagttgat gattcggtgg gcattcatag ccagggatcg gcggatgcag
    27241 ctcgtcgacc gtcagacgac ccggccagcg ttcgctcacc ggagccaatg catcgttgag
    27301 caaaccatta agttcattga gtgtcatgcc aggcagcgga cgaatatcca tatgcaactc
    27361 acaccaagcg caaatacggt tagaagcgtc gccaccgtga atatgcccga ggttgagcgt
    27421 agggtatggc acggtaaacg cttcgtagtg ataacgttct ttcaggttat cgcgcaattg
    27481 caaaatatgc ccgatggcgt cgtgcattag ttctatagcg ttaactccgc gtgctggatc
    27541 gctggagtgc cccgactggc cctgaatacg gatggcgtta gagatatgac ctttatgtgc
    27601 gcgtaccggt tgtagtgacg tcggttcgcc aatgatggcg caatccgggc gcagggcggt
    27661 agtttcggca aaataacgcg ctccggccat actggtttct tcatcagcag tcgccagaat
    27721 gtagagcggt tttttcagtt tcgtgacgtc gacatcgcgt agcgcatcaa ggataaacgc
    27781 aaaaaagcct ttcatgtcgg cggtgcctaa gccgtaaagc ttgccgtcat gctccgtcag
    27841 tgtaaacgga tcgcgcgtcc agcgaccgtc atcaaatggc accgtatcgg tatgccccgc
    27901 cagcaacaag ccgccagccc cctgtccggt acttgccagc atattaaatt tgttgcgagt
    27961 tcctggtaca ggctgcactt ccacattgaa gcctaaatct ttaaaccagt ccgccagcag
    28021 agtgattaaa tctgcattgc tttgatcgag tgcctcttcc gtggcgctta ttgaaggtgt
    28081 ggcaatcaga gcacggtaaa tctcgataaa tggcggtaat ttgtttttca ttgttgacac
    28141 acctctggtc atgatagtat caatattcat gcagtattta tgaataaaaa tacactaacg
    28201 ttgagcgtaa taaaacccac cagccgtaag gtgaatgttt tacgtttaac ctggcaacca
    28261 gacataagaa ggtgaatagc cccgatgttg aatacgctga ttgtgggtgc cagcggctac
    28321 gctggcgcag agctagtgac ctgtgtaaat cgccatccgc atatgaacat aaccgctttg
    28381 actgtttcag cgcaaagcaa tgatgcggga aagttaatct ccgatttgca tccgcagcta
    28441 aaaggcatcg ttgatctgcc gttgcagccg atgtcggata tcagcgagtt tagccctggg
    28501 gtggacgtgg tgtttctcgc caccgcccat gaagttagcc acgatttagc gccgcagttt
    28561 cttgacgcgg gctgcgtggt gttcgacctt tccggcgcgt ttcgtgttaa cgacgccacc
    28621 ttctatgaaa aatattacgg ctttacccat caatacccgg aactgctgga acaggcagcc
    28681 tacggtctgg cggagtggtg cggtaataaa ttaaaagaag cgaatttgat tgcggtgcca
    28741 ggctgttatc cgacggcagc acagttggcg ctgaaaccgt tgattgatgc cgatcttctt
    28801 gacctcaatc agtggccggt gatcaacgcc accagcggcg tgagcggtgc aggacgtaag
    28861 gcagcgattt caaatagttt ttgtgaagtt agcctgcaac cgtatggcgt ctttacccat
    28921 cgccatcaac cagagatcgc cacacacctc ggtgctgacg ttatcttcac cccgcatctg
    28981 ggcaatttcc cgcgcggcat tctcgaaacc attacctgcc gcctgaaacc gggtgtgacc
    29041 caggcgcaag tggcgcaagc gttacagcag gcgtatgccc ataaaccgct ggtgcggctg
    29101 tatgacaaag gcgttccggc gctgaaaaat gtcgttgggc taccattttg cgatatcggg
    29161 tttgccgttc agggcgaaca tctgattatt gtggcgaccg aagacaactt actgaaaggc
    29221 gcggcggcac aagcggtaca gtgcgccaat attcgtttcg gctatgcgga aacgcagtct
    29281 cttatttaag ggtgcaatga tgaatccatt aattatcaaa ctgggtggcg tactgctgga
    29341 tagtgaagag gcgctggaac gtctgtttag cgcactggtg aattatcgtg agtcacatca
    29401 gcgtccgctg gtgattgtgc acggcggcgg ttgcgtggtg gatgagctga tgaaagggct
    29461 gaatctgccg gtgaaaaaga aaaatggcct gcgggtgacg cctgctgatc agattgacat
    29521 tatcaccgga gcactggcgg gaacggcaaa taaaaccctg ttggcatggg cgaagaaaca
    29581 tcagattgcg gccgtaggtt tgtttctcgg tgacggcgac agcgtcaaag tgacccagct
    29641 tgatgaagag ttaggtcatg ttggactggc gcagccaggt tcgcctaagc ttatcaactc
    29701 cttgctggag aacggttatc tgccggtggt cagctccatt ggcgtaacag acgaagggca
    29761 actgatgaac gtcaatgccg accaggcggc aacggcgctg gcggcaacgc tgggcgcgga
    29821 tctgattttg ctctccgacg tcagtggcat tctcgacggc aaagggcaac gcattgccga
    29881 aatgaccgcc gcgaaagcag aacaactgat tgagcagggc attattactg acggcatgat
    29941 agtgaaagtg aacgcggcgc tggatgctgc gcgcacgttg ggccgtccgg tagatatcgc
    30001 ctcctggcgt catgcggagc agcttccggc actgtttaac ggtatgccga tgggtacgcg
    30061 gattttagct taagttttgt tggccggagg cgcagctttc cggcattgaa tttcaaaata
    30121 aggaaacaga gttatggcac tttggggcgg gcgttttacc caggcagcag atcaacggtt
    30181 caaacaattc aacgactcac tgcgctttga ttaccgtctg gcggagcagg atattgttgg
    30241 ctctgtggcc tggtccaaag ccctggtcac ggtcggcgtg ttaaccgcag aagagcaggc
    30301 gcaactggaa gaggcgctga acgtgctgct ggaagatgtt cgcgccaggc cacaacaaat
    30361 ccttgaaagc gacgccgaag atatccatag ctgggtggaa ggcaaactga tcgacaaagt
    30421 gggccagtta ggcaaaaagc tgcataccgg gcgtagtcgt aatgatcagg tagcgactga
    30481 cctgaaactg tggtgcaaag ataccgttag tgagttactg acggctaacc ggcagctgca
    30541 atcggcgctg gtggaaaccg cacaaaacaa tcaggacgcg gtcatgccag gttacactca
    30601 cctgcaacgc gcccagccgg tgacgttcgc gcactggtgc ctggcctatg ttgagatgct
    30661 ggcgcgtgat gaaagccgtt tgcaggatgc gcttaagcgt ctggatgtca gcccgttagg
    30721 ttgtggcgcg ctggcgggaa cggcttatga aatcgaccgt gaacagttag cgggctggct
    30781 gggatttgct tcagcaaccc gtaacagtct cgacagcgtt tctgaccgtg accacgtgct
    30841 ggaactgctt tctgctgccg ctatcggcat ggtgcatctg tcgcgttttg ctgaagatct
    30901 gattttcttt aacaccggcg aagcggggtt tgtggagctt tctgaccgcg tgacttccgg
    30961 ttcatcatta atgccgcaga agaaaaaccc ggatgcgctg gagctgattc gcggtaaatg
    31021 cggtcgggtg cagggcgcgt taaccggcat gatgatgacg ctgaaaggtt tgccgctggc
    31081 ttacaacaaa gatatgcagg aagacaaaga aggtctgttc gacgcgctcg atacctggtt
    31141 ggactgcctg catatggcgg cgctggtgct ggacggcatt caggtgaaac gcccgcgttg
    31201 ccaggaagcg gcgcaacagg gttacgcgaa cgccactgaa ctggcggatt atctggtggc
    31261 gaaaggtgtg ccgttccgcg aggcacacca tattgtgggt gaagctgtgg tggaagccat
    31321 tcgtcagggc aaaccgctgg aggagctgcc gctcaccgag ttgcagaaat ttagtccggt
    31381 gattggtgaa gatgtctatc cgattctgtc gctgcaatcg tgcctcgaca agcgtgcggc
    31441 aaaaggcggc gtctcaccac agcaggtggc gcaggcgatt gcttttgcaa gggcgcggtt
    31501 ggggtaagaa catttatatg tacaaatttg agcctggctt atcgccgggc ttttttatgg
    31561 caaaaaaaag cggatcctgg agatccgcaa aagttcacgt tggctttagt tattcgagtt
    31621 gagaaactct cgaaacgggc agtgacttca agggttaaaa gaggtgccgc tccgtttctg
    31681 tgagcaatta tcagtcagaa tgcttgatag ggataatcgt tcattgctat tctacctatc
    31741 gccatgaact atcgtggcga tggaggatgg ataatgaata ttcgtgatct tgagtacctg
    31801 gtggcattgg ctgaacaccg ccattttcgg cgtgcggcag attcctgcca cgttagccag
    31861 ccgacgctta gcgggcaaat tcgtaagctg gaagatgagc tgggcgtgat gttgctggag
    31921 cggaccagcc gtaaagtgtt gttcacccag gcgggaatgc tgctggtgga tcaggcgcgt
    31981 accgtgctgc gtgaggtgaa agtccttaaa gagatggcaa gccagcaggg cgagacgatg
    32041 tccggaccgc tgcatattgg tttgattccc acggttggac cgtacctgct accgcatatt
    32101 atcccgatgc tgcaccagac ctttccaaag ctggaaatgt atctgcatga agcacagacc
    32161 caccagttac tggcgcaact ggacagcggc aaactcgatt gcgtgatcct cgcgctggtg
    32221 aaagagagcg aagcattcat tgaagtgccg ttgtttgatg agccaatgtt gctggctatc
    32281 tatgaagacc acccgtgggc gaaccgcgaa tgcgtaccga tggccgatct ggcaggggaa
    32341 aaactgctga tgctggaaga tggtcactgt ttgcgcgatc aggcaatggg tttttgcttt
    32401 gaagccgggg cggatgaaga tacacacttc cgcgcgacca gcctggagac gctgcgcaac
    32461 atggtggcgg caggtagcgg gatcacttta ctgccagcgc tggctgtgcc gccggagcgc
    32521 aaacgcgatg gggttgttta tctgccgtgc attaagccgg aaccacgccg cactattggc
    32581 ctggtttatc gtcctggctc accgctgcgc agccgctatg agcagctggc agaggccatc
    32641 cgcgcaagaa tggatggcca tttcgataaa gtgttaaaac aggcggttta aaccgtttaa
    32701 cgcagctacc cgataggctt ccgccatcgt cgggtagtta aaggtggtgt tgacgaagta
    32761 ctcaatagtg ttgccgccac ctttctgttc cataatcgcc tgaccgatat gaataatttc
    32821 ggcagcgcgc tcgccaaagc agtgaatgcc cagaatctct tttgtttccc gatggaacaa
    32881 aattttcagc gtgcccacgt tcatgccgac gatttgtgca cgtgccagat gtttgaactg
    32941 ggcgcggccc acttcatatg gcactttcat cgcggtcagc tgctgttcgg ttttgcccac
    33001 agagctgatt tccgggatgg tgtaaatgcc ggtagggata tcttcaatca gatgcgcggt
    33061 ggcttcgcct tttaccagcg cctgcgcggc aatgcgcccc tggtcatagg ccgctgatgc
    33121 caggctcgga taaccaatca cgtcgcccac cgcgtaaacg tgcggctgtg cggtctgata
    33181 catgctgttg accttcagct gtccgcgact gtcagtttcc agcccaatgt tctgtaacgc
    33241 cagcgaatcg gtattaccgg tacgaccgtt ggcatagagc aggcagtcag ctttcagttt
    33301 tttacccgac ttcagatgca tgatcacacc gtcgtcacag ccttcgatct tctcgtactc
    33361 ttcgttgtga cgaatcacta cgccactgtt ccagaagtga taggagagag aatctgacat
    33421 ctcttgatcg agaaatgcca gcaggcgatc gcgggtgttg atcagatcca cttttacatc
    33481 cataccgcgg aagatcgacg catattcaca gccgatcact ccagcaccat agataagtac
    33541 atggcgcggt tcgtggtgca tgctgagaat tgagtcgctg tcgtaaatgc gtggatgggt
    33601 gaaatcaaca tctgttggat gatatggacg agagccgcag gcaataacaa atttttcagc
    33661 ggttagtgtt tcaacgctgc cgtccgggca atccagcgcc aacgtatgct cgtcaacaaa
    33721 gcgagcgttc ccctgcaata tttcacagtg attacgttcg taaaatccct gacgcatgcg
    33781 cgtttgttga ttaatcacgt tatcggcatg gttaaggata tcggcaaaag aagagcggag
    33841 cagtcgggaa tggtcgctgt atagtgggtt ttgattgaat tctataatgc ggctgacggc
    33901 gtgacggaga gctttcgacg ggatggtgcc ccagtgggtg caaccgccgc caacattttg
    33961 ataacgctcg ataactgcga cgcgcgcacc ttgcttaacc aggcccattg cagcgccttc
    34021 gccgccgggg ccggaaccta ttactatggc atcgtaatcg taggaatgtg gcatggtagg
    34081 gcttacctgt tcttatacat aaaagcaaca gaatggtaac attttatcgc gggtaagcca
    34141 attgatcccc gtcattaatc tggctatagc gtgagcggcc tttgctttgt ctgcctcctt
    34201 cttaacccat ttattcacta ttaatcgctt tttattatgg ctgatagttt tatcttgccg
    34261 aagcgtgctt ttttgttatt gtctttgtga aatcttctta taaaacaaac atataaata
//
LOCUS       DAZKIP010000040        33582 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZKIP010000040 DAZKIP010000000
VERSION     DAZKIP010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.040"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(22..639)
                     /gene="yncG"
                     /locus_tag="ACHOUK_002158"
     CDS             complement(22..639)
                     /gene="yncG"
                     /locus_tag="ACHOUK_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFP9897610.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQVPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWASDAPEQLKKNVIEYRKSLYVWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNATNISAIADAVCQLPTLQEILKRNEII"
     gene            906..2405
                     /gene="ansP"
                     /locus_tag="ACHOUK_002159"
     CDS             906..2405
                     /gene="ansP"
                     /locus_tag="ACHOUK_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFP9897611.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(2520..3581)
                     /gene="yncE"
                     /locus_tag="ACHOUK_002160"
     CDS             complement(2520..3581)
                     /gene="yncE"
                     /locus_tag="ACHOUK_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFP9897612.1"
                     /translation="MHLRHLFSLRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKDSVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            3823..5925
                     /gene="pqqU"
                     /locus_tag="ACHOUK_002161"
     CDS             3823..5925
                     /gene="pqqU"
                     /locus_tag="ACHOUK_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFP9897613.1"
                     /translation="MKIVFVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDDASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSSRDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGSVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGMEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGRNYGVGVNIAWRFE"
     gene            complement(5961..6626)
                     /gene="mcbR"
                     /locus_tag="ACHOUK_002162"
     CDS             complement(5961..6626)
                     /gene="mcbR"
                     /locus_tag="ACHOUK_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFP9897614.1"
                     /translation="MPGTEKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(6824..7861)
                     /gene="curA"
                     /locus_tag="ACHOUK_002163"
     CDS             complement(6824..7861)
                     /gene="curA"
                     /locus_tag="ACHOUK_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFP9897615.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQSGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQREMGQWVKEDKIHYREEITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            8042..8560
                     /gene="mddA"
                     /locus_tag="ACHOUK_002164"
     CDS             8042..8560
                     /gene="mddA"
                     /locus_tag="ACHOUK_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFP9897616.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            8557..9006
                     /gene="ydcZ"
                     /locus_tag="ACHOUK_002165"
     CDS             8557..9006
                     /gene="ydcZ"
                     /locus_tag="ACHOUK_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFP9897617.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNSLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(9007..9240)
                     /gene="ydcY"
                     /locus_tag="ACHOUK_002166"
     CDS             complement(9007..9240)
                     /gene="ydcY"
                     /locus_tag="ACHOUK_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFP9897618.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(9326..9499)
                     /gene="ortT"
                     /locus_tag="ACHOUK_002167"
     CDS             complement(9326..9499)
                     /gene="ortT"
                     /locus_tag="ACHOUK_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFP9897619.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            9694..9789
                     /gene="yncL"
                     /locus_tag="ACHOUK_002168"
     CDS             9694..9789
                     /gene="yncL"
                     /locus_tag="ACHOUK_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFP9897620.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(9886..11310)
                     /gene="patD"
                     /locus_tag="ACHOUK_002169"
     CDS             complement(9886..11310)
                     /gene="patD"
                     /locus_tag="ACHOUK_002169"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFP9897621.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTVACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(11332..12126)
                     /gene="ydcV"
                     /locus_tag="ACHOUK_002170"
     CDS             complement(11332..12126)
                     /gene="ydcV"
                     /locus_tag="ACHOUK_002170"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9897622.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLMALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(12116..13057)
                     /gene="ydcU"
                     /locus_tag="ACHOUK_002171"
     CDS             complement(12116..13057)
                     /gene="ydcU"
                     /locus_tag="ACHOUK_002171"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9897623.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            complement(13058..14071)
                     /gene="ydcT"
                     /locus_tag="ACHOUK_002172"
     CDS             complement(13058..14071)
                     /gene="ydcT"
                     /locus_tag="ACHOUK_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9897624.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFICVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            complement(14089..15234)
                     /gene="ydcS"
                     /locus_tag="ACHOUK_002173"
     CDS             complement(14089..15234)
                     /gene="ydcS"
                     /locus_tag="ACHOUK_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFP9897625.1"
                     /translation="MSKTFARSSLCALSMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLQDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRDQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQTNALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASSL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            complement(15479..16885)
                     /gene="ydcR"
                     /locus_tag="ACHOUK_002174"
     CDS             complement(15479..16885)
                     /gene="ydcR"
                     /locus_tag="ACHOUK_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFP9897626.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKVPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQEYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWLWG
                     EREEQAVKQLGKLIQERL"
     gene            complement(16964..17380)
                     /gene="hicB"
                     /locus_tag="ACHOUK_002175"
     CDS             complement(16964..17380)
                     /gene="hicB"
                     /locus_tag="ACHOUK_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFP9897627.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            complement(17426..17602)
                     /gene="hicA"
                     /locus_tag="ACHOUK_002176"
     CDS             complement(17426..17602)
                     /gene="hicA"
                     /locus_tag="ACHOUK_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HFP9897628.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIK
                     EPLRKAILKQLGLS"
     gene            17824..18054
                     /gene="yncJ"
                     /locus_tag="ACHOUK_002177"
     CDS             17824..18054
                     /gene="yncJ"
                     /locus_tag="ACHOUK_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFP9897629.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            complement(18146..20107)
                     /gene="rlhA"
                     /locus_tag="ACHOUK_002178"
     CDS             complement(18146..20107)
                     /gene="rlhA"
                     /locus_tag="ACHOUK_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFP9897630.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVVKVAKDHLDV
                     AVTEPLANGDGLNVLIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(20180..20716)
                     /gene="sutR"
                     /locus_tag="ACHOUK_002179"
     CDS             complement(20180..20716)
                     /gene="sutR"
                     /locus_tag="ACHOUK_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310064.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFP9897631.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVIISLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            20769..21980
                     /gene="ydcO"
                     /locus_tag="ACHOUK_002180"
     CDS             20769..21980
                     /gene="ydcO"
                     /locus_tag="ACHOUK_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFP9897632.1"
                     /translation="MLRCLYAITHEVTMRLFSVPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLLAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNR"
     gene            complement(22015..22683)
                     /gene="ydcL"
                     /locus_tag="ACHOUK_002181"
     CDS             complement(22015..22683)
                     /gene="ydcL"
                     /locus_tag="ACHOUK_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFP9897633.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(22987..23580)
                     /gene="tehB"
                     /locus_tag="ACHOUK_002182"
     CDS             complement(22987..23580)
                     /gene="tehB"
                     /locus_tag="ACHOUK_002182"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFP9897634.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGEYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWEMVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(23577..24569)
                     /gene="tehA"
                     /locus_tag="ACHOUK_002183"
     CDS             complement(23577..24569)
                     /gene="tehA"
                     /locus_tag="ACHOUK_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFP9897635.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTLALLMQGKLLVRTERAVLMKAEDKE"
     gene            24693..25673
                     /gene="ydcK"
                     /locus_tag="ACHOUK_002184"
     CDS             24693..25673
                     /gene="ydcK"
                     /locus_tag="ACHOUK_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFP9897636.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     INQGAYISDSVTIHDSLVCGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVIRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            complement(25668..26204)
                     /gene="rimL"
                     /locus_tag="ACHOUK_002185"
                     /pseudo
     CDS             complement(25668..26204)
                     /gene="rimL"
                     /locus_tag="ACHOUK_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
     gene            complement(26267..26491)
                     /gene="ydcH"
                     /locus_tag="ACHOUK_002186"
     CDS             complement(26267..26491)
                     /gene="ydcH"
                     /locus_tag="ACHOUK_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFP9897637.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDDRGY
                     NAEVVRMKKQKLQLKDEMLKILQHESVKEV"
     gene            complement(26631..28286)
                     /gene="opgD"
                     /locus_tag="ACHOUK_002187"
                     /gene_synonym="mdoD"
     CDS             complement(26631..28286)
                     /gene="opgD"
                     /locus_tag="ACHOUK_002187"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFP9897638.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(28511..29854)
                     /gene="ydcJ"
                     /locus_tag="ACHOUK_002188"
     CDS             complement(28511..29854)
                     /gene="ydcJ"
                     /locus_tag="ACHOUK_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9897639.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYDQRGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQARSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            30071..30994
                     /gene="ydcI"
                     /locus_tag="ACHOUK_002189"
     CDS             30071..30994
                     /gene="ydcI"
                     /locus_tag="ACHOUK_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9897640.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGPNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(31032..32672)
                     /gene="trg"
                     /locus_tag="ACHOUK_002190"
     CDS             complement(31032..32672)
                     /gene="trg"
                     /locus_tag="ACHOUK_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFP9897641.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRTLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHYA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLNKQSVLAEPRGAGEPVSFAPV"
     gene            33071..33220
                     /gene="hokB"
                     /locus_tag="ACHOUK_002191"
     CDS             33071..33220
                     /gene="hokB"
                     /locus_tag="ACHOUK_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFP9897642.1"
                     /translation="MKRNPLVVCLLIICITILTFTLLTRQTLYELRFRDSDKEVAALM
                     ACTSR"
     gene            complement(33198..33266)
                     /gene="yncP"
                     /locus_tag="ACHOUK_002192"
     CDS             complement(33198..33266)
                     /gene="yncP"
                     /locus_tag="ACHOUK_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFP9897643.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(33292..33465)
                     /locus_tag="ACHOUK_002193"
     CDS             complement(33292..33465)
                     /locus_tag="ACHOUK_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9897644.1"
                     /translation="MKKLALILFMGALVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
BASE COUNT         8302 a         8596 c         8121 g         8563 t
ORIGIN      
        1 cataatcttc tggtatccca ctcaaataat ttcattcctt tttagtattt cttgtaacgt
       61 tggtaactga catactgcat cagcaatcgc actgatattc gtggcattat cctgaaacca
      121 ttcatgccca ggcccccatg tgcgcatagt gcaaagataa cagtccacca gcgttagttg
      181 ctcaccaaac gcataaggtt cagcggtcaa ttgtgaattc agccagacat aaagcgattt
      241 acgatattca ataacattct tctttaactg ttcaggtgcg tcagatgccc aacgctcagg
      301 gtaatcggcg aaagtgaacg ttggatagac attagcaacc agccagacca ataaccgttg
      361 aaactgctga cgttcggcac gcccaaccgg tggcgcaagg tccgggcgac gatcgagaac
      421 catcaatgca atcgccgctg tctccgtcat aatctcgtca ttttccagtg ccagtgtcgg
      481 aacctgacac aacgggttca gggttttcaa taactcgcgt gaagttccct cgtggtcaaa
      541 accactgaca tcaacgaact gataaggaat atcagccagt gttagcatca gctcactgat
      601 tgtagagccc catccgggta cgccatacac cttaatcata tgcccctcac aatcttttaa
      661 agattaaatg taggacagta caaaggttaa ccagcaaaag gatttgtcat tttttcgtca
      721 gttgtattaa tccccttcag gattgtaagg ggatgttttg catcgtttat cgataacggg
      781 taggatgctg cgccgatagt tttataccca taaagaataa tggtgataac tatcatcgcc
      841 aggatgaata aacattgttc atggcaactt atatgacttt ttcattaaag caatcaggga
      901 gagcaatgag taaacacgac accgacactt cagatcaaca cgccgcgaaa cgccgctggc
      961 ttaatgccca cgaagagggg tatcacaaag cgatgggcaa tcgccaggta cagatgatcg
     1021 ccattggcgg cgcgattggc accggcttgt ttttaggtgc aggagcccga ctgcaaatgg
     1081 cgggtccagc actggcactg gtttatttaa tttgtggctt gttttcgttt tttattctgc
     1141 gtgcattggg tgagctggtg ctacaccgcc cttccagtgg cagttttgtc tcttatgccc
     1201 gtgagttttt gggtgagaaa gccgcttatg ttgctggctg gatgtacttc atcaactggg
     1261 cgatgacggg gattgttgat attaccgccg tcgctctgta tatgcattac tggggtgcgt
     1321 ttggcggcgt gccgcagtgg gtctttgcgc tcgctgcact taccatcgtt ggcaccatga
     1381 atatgatcgg tgtgaaatgg tttgcggaga tggagttctg gtttgcgctt attaaagtgc
     1441 tcgccattgt gacctttttg gtcgtgggta cggtgttcct cggtagtggt cagccgctgg
     1501 atggcaacac cactggcttt catttaatca ccgataatgg cggcttcttc ccccacggtt
     1561 tgctgcctgc gctggtgttg attcagggcg tagtgtttgc ttttgcctcc attgaaatgg
     1621 tgggtacagc tgccggagaa tgtaaagatc cgcagaccat ggtgcctaaa gcgattaaca
     1681 gtgtgatttg gcgtattggc ctgttttacg tcggctccgt ggtgttgctg gttatgttat
     1741 tgccgtggag cgcgtatcag gcggggcaaa gtccgttcgt gacgtttttc tctaaactgg
     1801 gtgtgccata tatcggcagc attatgaaca ttgtggtgct gaccgctgcc ctctccagcc
     1861 tgaattcagg tctgtactgc accggacgta ttctgcgctc aatggcgatg ggcggttccg
     1921 caccgagttt tatggcgaaa atgagtcgtc agcatgtgcc gtatgccggg attctggcga
     1981 cactggttgt gtatgtcgtc ggcgtattcc tcaactatct ggtgccgtcg cgcgtatttg
     2041 agattgtgtt gaacttcgcg tcgctgggaa tcatcgcttc atgggcgttt atcatcgtgt
     2101 gccagatgcg cctgcgtaaa gcgattaaag aaggcaaagc agcggatgtc agttttaaac
     2161 tgcctggcgc gcccttcact tcctggctga cattactgtt tttactgagt gtccttgtgc
     2221 tgatggcgtt cgattacccg aacgggactt atactatcgc ggcgctgccg attatcggta
     2281 ttctgctggt tataggctgg tttggtgtgc gcaaacgcgt tgctgaaatt cacagcactg
     2341 cgccagtcgt cgaggaagat gaagaaaaac aggaaattgt gtttaagcct gaaacggcga
     2401 gctaattgca aaattgcccg gctacgtgcc gggcaaattt agattactga cgaaggtgtg
     2461 ccgcatgcgg cgtgatcaaa gtcaggcaag tctctccgtt atcaccctcg ccatatctat
     2521 tacagcgcaa tacgaatcac atcgtctggc tgggtagctt cctgctgttt agtggatttt
     2581 tgtttcacac tgacatacag cgttttgcca tcggcagaca gcgccaggct gttcggatga
     2641 gtcggcgtat cgaacgtttt caccacttta tagcttttcg cgtcaatcac actgacttta
     2701 cctgcctgac gatgcgttac gtaggcttca ttacgcgctg ggttaaacag cacagccagt
     2761 gattccggtg ccgcaacctt cgccagaata ttgccattac gggtatcgac cactaacact
     2821 tctgccgctt tagaatcggt gataaatgca cgctgcctgg cggtatcaag gctgatgttg
     2881 ataaagaagt gctctttgcc gtcatccagc agctttttac ggctgaggat tttattgtcg
     2941 gcggtgtcga tggtaatcaa ttcgccgtca gcgttagtgg tgtaaagacg tttgcctttg
     3001 ctatccagtg ccagaccggt actcatttta ccggtgttct ggatggcggt tttcagtttg
     3061 atattctcgc catcaacgac ccaaatcacg ctatctttac caataccact gatgtaaacg
     3121 gtgttcgtgg catcatcagc taccagctca cgcggttgca gcgggcgcac ctcttccgtg
     3181 cgcttacgat catccagcac cagacggcct ttcacctcgc cagttttggc atctatcgcc
     3241 gtgaccgcgc tgtttacggt gttaccaaac cacaacgtct gagtcgtgtt attgatggtg
     3301 gcaccaaacg gcttgagatc gttatggatc gcctgcgtca cttccagagt gaccggatca
     3361 agacgataaa ccacgccgcc tttatccagt ttgcggcttt gcgaagtggc gagccacagc
     3421 gcgttttctt gctggctata agccatttcg taggcacctt tacctaccgc tttacgcagc
     3481 atttcttctg cggcctgcgt actgaatgat gaagcaacaa gcaatgaacc taacagtaat
     3541 gaaccacgca ggcgcaatga aaacagatga cgtaaatgca tgacgactcc ctttgatgaa
     3601 ccgatatttt tgctgcttca caatgcttcg ctaagcgtaa tcatcgttac gctcttgtta
     3661 taaatgggaa tggtaatcat tatttactca aatgtggagc atttcttgcc gttacgacta
     3721 caattctctc aattaattaa cgtatcgtgc cgtttcgttt tccataaatt tacaatttca
     3781 ttaccatgac ggtgttttcc cattcttaac ctggcttttt tcatgaagat tgttttcgtt
     3841 cgacagaccg ttttgcccgc cctgcttgtc ctttcccccg ttgtttttgc cgctgatgaa
     3901 cagaccatga ttgtcagtgc cgcgccgcag gtggtttcag aactggatac ccctgcagca
     3961 gtaagcgtgg tggatggcga ggagatgcgc ctggcaacac cgcgcattaa cttgtccgaa
     4021 tcactgaccg gcgtgcctgg tttgcaggta caaaaccggc agaactatgc gcaagattta
     4081 cagctgtcga ttcgtggatt tggctcccgc tccacttacg gaattcgcgg tattcgcctg
     4141 tatgtggacg gtattcccgc taccatgccc gacgggcaag ggcaaacatc caacatcgat
     4201 ttaagcagtg tgcaaaatgt ggaagtgctg cgtggcccct tctctgccct gtatggcaac
     4261 gcgtctggcg gtgtaatgaa tgtcaccacc cagaccgggc aacagccacc aaccattgaa
     4321 gccagtagtt actacggcag ttttggcagc tggcgctatg ggctgaaagc aacgggcgca
     4381 acgggagacg gcacacagcc tggcgatgtt gattacaccg tctcaaccac gcgttttacg
     4441 acccacggct atcgtgacca tagtggcgca cagaaaaatt tagctaatgc caaactgggc
     4501 gtacgcattg atgacgccag caaattaagc ctgattttca atagcgtgga tatcaaagca
     4561 gatgacccag gtgggctaac caaagcagaa tggaaagcga atccgcaaca agcgcctcgt
     4621 gcagaacagt acgacacgcg aaaaaccatc aagcaaactc aggctgggtt gcgctatgaa
     4681 cgtagcctga gttcgcggga tgatatgagt gtgatgatgt atgccggaga gcgagaaacg
     4741 acccagtacc agtcaatacc catggcacca caacttaacc cgtcacatgc gggcagcgtg
     4801 attaccctgc aacgccatta ccagggaata gacagccgct ggacacaccg tggtgaactg
     4861 ggcgttccgg tcacgttcac taccggcctg aactacgaaa acatgagtga aaaccgcaag
     4921 ggctacaata acttccgcct gaatagcggc atgccggagt acgggcaaaa aggtgagttg
     4981 cgtcgcgacg aacgcaatct gatgtggaac atcgatccct atttacagac gcagtggcag
     5041 ctgagcgaaa aactgtcgct ggatgctggc gtgcgctaca gctccgtgtg gtttgattcc
     5101 aacgaccatt acgttactcc gggtaacggc gatgacagcg gtgatgccag ttatcacaaa
     5161 tggctaccag ccggatcgtt aaaatatgca atgaccgatg cctggaatat ctatctggca
     5221 gccgggcgtg gttttgaaac gccgacgatt aatgagctgt cttatcgcgc tgatgggcaa
     5281 agcggtatga actttggttt aaaaccatcc accaacgata caattgagat cggcagtaaa
     5341 acgcgtattg gtgatgggct gcttagtctc gcattgttcc agaccgacac cgatgatgaa
     5401 attgttgtcg atagcagtag cggtgggcgt acgacttaca aaaatgctgg aaagacccgt
     5461 cgtcaaggcg ctgaactggc atgggatcaa cgtttcgcgg gagattttcg cgtaaacgcg
     5521 tcctggacct ggcttgatgc gacctatcgc agcaatgttt gcaacgaaca ggattgtaac
     5581 ggtaatcgga tgccagggat cgcccgtaat atgggctttg cgtcgatagg ttatgtaccg
     5641 gaagatggtt ggtatgcagg catggaagcg cgttatatgg gcgatattat ggcagatgat
     5701 gaaaatacgg ccaaagcgcc gtcttatact ctcgtcggct tattcaccgg gtataaatac
     5761 aattaccaca atttaactgt ggatttgttt ggtcgtgtcg ataatttatt cgataaagaa
     5821 tacgttggtt ctgtcattgt caatgagtca aacgggcgat attacgaacc tgcgcccggg
     5881 cgaaattatg gtgtcggcgt gaatatcgca tggcgatttg agtaattacc ttgatgcccg
     5941 gtatttgccg ggcatttact ttaacgattg tattgctggt ataaaatagc aatattttgt
     6001 tgcataattt cagcaagaca atgtctgcat ccctctttgt cttttgcttt taacgcagcg
     6061 agtaacagat gatagttttc tatatgctcc cgtaattccg ctggattaat cgcttcatag
     6121 agataatgta aaccaggtcc catcctgacc cacagttgct caatcatctc acacaggatg
     6181 ggcatatttg agcgatgata aatcgctaag cgaaatagcc tgtttacatt aatgatttgt
     6241 tccatgtcgc ccttttcttg cgcctgctgt aatttctcga gcaattcctg gagttccgcc
     6301 aggtcttgcg gggtgaggtt ttcaacagcc agagcaactg ccattaattc cagttcgtag
     6361 cggatccgat tgatttcgtc taattgacgt ttccccactt ccggaactgt aaatgcttgc
     6421 gctggtgcga cagaaagcgc attcaccgaa accagacgta ataatgcttc acgcacaggt
     6481 gtaatactca tacctaattg ctccgccaga tttttagtaa tcaggcgtgc gccaggtttt
     6541 agtgcgccaa tactaagctg atgtttcagg tcgttctcaa cctgcagagt caaactgaca
     6601 tgtttcattt tttccgttcc aggcatttta attccttcgc gtgttttctg tccaaccctg
     6661 cctgcatggc agaaggaatt cacctggtgt attaaagtga ataatcataa accactcacc
     6721 tttgttgtta ttagcagcag aacaatgatg tggttagtta attaaagctg aaaataagct
     6781 ttttgcctgg atttttatgg cggcttacgc cgccgttgaa actttaatca tcacccgcca
     6841 cgcggatcac cactttgccg aagtttttac ccttcagcag gccgataaac gtctgtggcg
     6901 cattctctaa accgtcagta atttcttcgc ggtagtggat tttatcctct ttcacccatt
     6961 gccccatctc cctctgaaac tcatggatgc ggtgaccata atcctgagcg ataataaaac
     7021 cttgcaagcg aatacgtttt ttcagcactg tagccatcaa cagaggtaaa cgatccggac
     7081 cgggtggtag ctctgtagcg ttatagctgc tcactaatcc gcagacggga atgcgcgcag
     7141 atgtattaag taacggtagc accgcatcga ataccttacc gcccacgttt tcataataga
     7201 tatcaatacc ttttgggcac gctttcgcca gttgttcggc aaaatcatcc gcgtggtgat
     7261 caagacaaac atcgaagcct aacacctcgg tagcatggcg gcatttttcc gcgccaccgg
     7321 ctacccccac cactctgcaa cctttaagtt tgccgatttg ccccaccgtc gcccccactg
     7381 gtcctgtcgc cgcagctacc accaacgttt cgccctcttt aggctgaccg atatccagta
     7441 ggcccatata agcggtaaag cctggcatcc ctagcacacc cagcgaccac gatggatttt
     7501 gcggatgatc gccaagtttc accagatcat caccactgga tatgtcatag tcttgccatc
     7561 cactgtagcc cagcacccag tcgccagact gataatcagg atgattcgac tccacgacac
     7621 ggctcaccgt accgccgacc atcacgccgc caatatcaac cggcggtgaa taagatggct
     7681 catcgctcat acgtccacgc atatacgggt ccagggacaa ataaactgtg cgcagtaaca
     7741 cctgtccttc acccggtgtg gcgacatcat cttcttcaag acggaaattc tccggaaccg
     7801 gtgcgccatg tggacgcgag gccagaaccc aacgtcgatt acgctgcttt tgttgcccca
     7861 taatgctctc ctgtgctttt ggcatgaatc catcaatagt agcaggctcg caggattggt
     7921 tttgtttgca gccgtcaccc cccctctgca acacgcaata atttcatcga ggaatccgtt
     7981 gcttcgggct gttttcgata gtattgataa catcatcatc attattttat tgaggccaga
     8041 catgtccatc cgttttgccc gcaaagccga ctgtgctgcc attgcggaaa tttataacca
     8101 cgccgtgttg tatacggcgg ctatctggaa tgaccaaacg gtggatgcag ataaccgcat
     8161 tgcctggttt gaagcgcgga ccatagcagg ttatccggtg ctggtaagtg aagaagatgg
     8221 cgtagtgacg ggatatgcct catttggcga ctggcgtagt ttcgatggtt ttcgccatac
     8281 cgtggaacat tcggtttatg tccatcccga tcatcagggc aaaggtctgg ggcgtaaatt
     8341 gttaagccga ttgattgatg aagcgcgaga ttgcgggaag catgtcatgg tcgccgggat
     8401 cgaatcgcaa aatcaggcct cgctgcatct gcaccagtcg ctgggatttg tcgtcaccgc
     8461 acaaatgccg caggtgggca ctaaatttgg gcgctggctg gatctgacat ttatgcagtt
     8521 gcaactcgac gaacgcactg aaccggacgc gattggatga atcagtcgct cacccttgcc
     8581 tttttgattg ccgccggaat tggtctggta gtccagaact cgctgatggt gcgcatcacc
     8641 cagacctcct ctaccattct catcgccatg ttgctgaact cactggtggg gattgtgctc
     8701 tttgtttcta ttttatggtt taaacaaggt atggcggggt ttggcgaact ggtgtccagc
     8761 gtgcgctggt ggacacttat acctggcttg ctgggatcgt tttttgtctt cgccagtatc
     8821 agcggatatc agaatgtggg agcggcgaca actattgcag tactggtcgc cagccagctg
     8881 attggcggtc tggttctgga tatcttccgc agtcatggcg taccactacg ggcgttgttc
     8941 ggcccaattt gtggcgcaat tttacttgtg gttggcgcct ggttggtagc cagacgctca
     9001 ttttaatcac aggatggtgc cgcctttggt taactgttca tggcgcgctt ccatatcttc
     9061 tttatgcttg cgaccgtgat gagcaatagc ggtgcgcaaa cgctgctgct gggtgtagcg
     9121 atcttcccga cttaactccg cgtcatcgct cagggcgatt aataattcgt tcatatgggc
     9181 aatgacgctc tcttcaatcg cggcatccac gcgcgcgatg acttcatcca gatgcgacat
     9241 actttctcct tagccacttg tgaaatttat cgattcatcc aaatgatata agtctgcgtg
     9301 gtgccgtcaa gccagtgagt aagatttaaa acagtgaaaa caacagcgcc accgggaaac
     9361 tcatcggcca tgttgccccc accagaaagg cgcttaagaa acggatgcgt ttcttatcgt
     9421 gagaaagaaa ccaagtgata agaaatgcga ttgctgccat aaccgcataa aaaacaagca
     9481 tgtgttgata gagagacatg tacgggcgtc cagccagatg aataaaacgc gcgcaaatat
     9541 gcgtcatttt ttgacgtgca tcaagttttt taaccggttt gagaaaaaaa catcaaacag
     9601 caacttgccc ttatccatct tcagaatgag tattattgcg gattttctta acccgtacta
     9661 tacacatagg ggcaatgata aaaggtggca aaaatgaatg tttccagtag aactgtagta
     9721 ctgataaatg tctttgctgc tgttggtttg tttactctta tctctatgag atttggctgg
     9781 tttatttgat gtagtgattt tgcacgcagc acgtactgtc agagaaatca gtgctctgac
     9841 agtacgcaag atcatctcat actcttacga tctgcaatat tatccttaat gtttaaccat
     9901 gacgtggcgg acgacggtgt aatcctccag cccataaagt gacatatcct tgccgtaacc
     9961 agaaagtttc tgcccaccgt gcggcatttc acttaccagc atgaaatggg tattgaccca
    10021 ggtacaacca tattgcagcc gtgcactgac gcgatgcgcc ctgcccacat ctttcgtcca
    10081 taccgaagat gcaagtccgt actggctgtc attcgcccag tttaccacct gttcttcgtt
    10141 gtcgaagagc gtaacactca ctactggacc aaatacctct ttttgcacga tggcatcgtc
    10201 ctgtaatgcg ccagccagca gcgtcggcgc atagtaatag ccattaccct tgcgcttttc
    10261 accgccagtg atcactttga tgtgccctgt cgctttcgcc tcttctactg ccttgctgac
    10321 gcgttcgaga tgcgccagcg agcttaaagg tccaagctcc gtagactcgt catccggcgc
    10381 accagatttt aacgttgcca ctgcagcacc gagtttttcc accagcgtat cgtaaatgcc
    10441 tttttgcgcg tagatccgac aagccacagt acaatcctgt ccggcattgt aatagccaaa
    10501 tgtacgtaca ccttcgacca ctgcttcaat atccgcatca tcaaaaacaa tcactggcgc
    10561 tttgccacca agttccatat gagtacgctt aatggagggc gcggtatggc tgatgatgtg
    10621 ctcgccggtg gcgatagagc ccgtcagcga caccatccgc actttgggat gaccggtcag
    10681 cggatcgccc accgttttgc ctctgccaaa cagtacgtta atcacgcctg ccgggaagat
    10741 atctttcgcc agctctgcca acttcaacgc ggtcagcggg gtaatttctg atggtttaag
    10801 cactacgcag ttccctgccg ccagcgccgg agccagtttc cacgctgcca tcatcagcgg
    10861 ataattccac ggtgcgatag aagccacgac ccccaacgga tcgcgacgga tcatcgaagt
    10921 atgaccttca agatattcac ctgccgccag accattcaga cagcgcgccg cacccgcgaa
    10981 aaagcgaaaa acatcgacaa tcgccgggat ttcatcattg aacgcactat gcagcggttt
    11041 gccacaatta cgggactcca gttcggcaaa aacctgacca ttttcttcga taacatcagc
    11101 cagtttcagc agacattccg cacgcacttt cggcgtggtt tgcccccatt cggcaaatgc
    11161 tgcatctgcc gcgcgcacag cagcatcgac ctgctctgcg gatgcctcgg caatctccag
    11221 taaaacgtcc cccgttgccg gattatagac aggctgtttt tccccttcgc cgctaaccag
    11281 ttctccgtta atcagtaact tatgttgcat agcatattcc tgtatcagtg gttattgacc
    11341 attgtcgcct tcgcgggtta gccaccaggc tcccaggatc ggcaaggttg ttaccaacat
    11401 aaccagcagt gccaccacgt tagttaccgg tacatcacgc ggtcgcccaa gctgattgag
    11461 caaccacaac ggtaacgttc gttcatgacc tgccgtaaag gtcgtaacga tgatttcatc
    11521 gaacgacaag gcaaacgcca gcattcctcc tgccagtaac gccgaactga gattcggcaa
    11581 cactacgtag cggaaggttt gccagccatt ggccccaaga tccattgacg cctcaaccag
    11641 actccatgag gtgcggcgaa aacgggcgat gacattgtta aacaccacaa ctacacaaaa
    11701 agtcgcatga ccgaccacga tggtgaaaaa tcccggctcc agattgatgg ttttaaaggc
    11761 ggttaataac gccagaccag tgacaatgcc cggcagcgca atgggcagca gcagtaacag
    11821 cgaaatggcg tttttgccga aaaagtctcg tcgccacagc gcggcagctg ccagcgtccc
    11881 taacaccagc gccattaatg tcgccagcgc cgccacttta agtgacagtg tcacggcatc
    11941 aagaatatca ctacgctgtg ctgccacgct aaaccagcgc agcgtcaggc cctgcggtgg
    12001 aaaactaaac gccgcatctt cagtgttaaa cgcataggcg gcgattatca ggatgggaaa
    12061 atgtaggaaa acaacgccgc cccaggccgc cagtttgagg aaaaacggtg cgcgttcaga
    12121 gtgcatcaaa cgctcccaga cgtttcacga acgccaggta cagtgcgata agaacaatcg
    12181 gcaccagggt gaatgccgcc gccatcggca tattgccaat cgccccctgc tgggaataaa
    12241 ccatattgcc gataaaatat cctggcgggc caaccagctg cgggacgata aaatcgccca
    12301 gtgtgagtga gaaggtaaag atagagccag cggcaatacc cgggatcgcc agcggcaaca
    12361 ccacatagcg aaaggtttgt cgtggacgtg cgccgagatc agctgacgcc tgcaacaatg
    12421 acggcggcaa acgctcaagc gccgcctgca cgggcaggat catgaacggc agccagatat
    12481 agagaaacac cagaaagcgc cccagcccgg aagttgacag cgtattgccg cccaccgcag
    12541 gtaatgtaag gaacgctgtc agcagtggtt ccagcccaag atgttgtaaa aaccactgag
    12601 ccacgccatc ttttgccagc aataacgtcc aggcataggc tttaacaatg tagctcgccc
    12661 acatcggcaa cattaccgca atataaaaaa acgctttcat tttcccgctg gtatagcgcg
    12721 ccatatacca cgccattgga aaagccagaa tggcgctggc gatagtgact gccacagcca
    12781 tagtcagcgt gcggagaatg atgtcgtaat tcgccggatt aaacagcgca cggatgttcg
    12841 ccagcgtcag ttccggcgtt accgacatgg tgaaatcgtc aaaggtataa aagccctgcc
    12901 ataacagcgt tagcagcgag ccgaaataga caatgccaaa ccacattagc gggccaagca
    12961 gcagcaaaaa cagccccagc cccggattcc gccagaagaa accggacacc ttacccagac
    13021 ctggacgtga aggtgattgc aatacgttca tcgccattca cctctcctca accagcggca
    13081 ccatcacatc acgcgaccag gaaaccatca cctgttgtcc gggcgtgagc gtggcaggca
    13141 gttcttcgcc tgtcatattg gcctgactca ccagcagttt ttcaccgccg ttcaatttca
    13201 gttcaaaacg agttgccgcg ccctgatatt gcaccgcctg gatcgtgcca ttggcctgca
    13261 gttcaccagg ggtgttgagg cggatatgtt ccggccgcag ggcgaagctt cccgtcatgc
    13321 cacaaagttt ctctgccatc agtccatcaa aaacattcga tgtaccaacg aacccggcaa
    13381 caaacggcgt gcgcgggcgc atatagagat cgcgcgggga atcgacctgc tcaatgcgtc
    13441 cattattgaa aaccgccaca cgatcggaca tcgataacgc ttcgccctga tcgtgggtaa
    13501 cgcagataaa agtgataccg agagactgtt gcagtttttt cagctcaagc tgcatctgct
    13561 cacgcaattt gagatccagt gcgccgagcg gttcatccaa cagcaatacg cgcggttcat
    13621 tcaccaatgc tctggcgata gcaacccgct ggcgctgacc accagaaagt tgtgacggtt
    13681 tacgttgctg tacaaacccc aacgccactt tctccagcgc ctcttgcgcc attgcgtgcc
    13741 gctgcttttt attcacgcct ttgaccatca gcccataggc gacattgtca agaatcgaca
    13801 tatgcggaaa tagcgcgtag tcctgaaaga cagtattcac gtcccgctcc cacggtggca
    13861 gattgctggc gggtttacca aagatagaga tagccccgcc ggaaagctgt tcgaagccag
    13921 caatcaggcg caggcaggtg gttttgccgg agccggacgg ccccagcata gagaagaact
    13981 caccatcttt tatcgcaata ctgacgccat ctactgcgcg cacgtcaccg tacaaccgcg
    14041 agacgttgtc aaactccact gcgtacgtca taaagcaccc cagcgaagtt agcgaccgcc
    14101 cataatggca atgtaatcct gcgtccagcg actgtaggga acaaacttgc ccccttctgc
    14161 tataggcgtt ttccagaagg ctattttatc gaaatagtta aaaccgtttg tttcacaacc
    14221 tttctcgcct aataacgaac tggctttaca cccttccggc acgactggta acgagccaaa
    14281 ccaggccgcc acatcgccct gcacttttgg cgttaatgac cagttcatcc atttgtaggc
    14341 gcaaaccgga tgtttcgctt cgctatgtag catggtggtg tcagcccaac cggtaacgcc
    14401 ctccttcggg aaaacggtag cgacaggctg accttcggct ttcagggcgt tggtctgata
    14461 gggccaggca ctggaagcga ccacaccctc gtttttgaaa tcgctcattt gcacggtagt
    14521 gtcatgccag tagcgatgga tcaaactatg ttgatcacgc agcactttca gcaccgcctg
    14581 gtactgttct tcggtgagtt gatacggatc gctgatgccc aactgcggct gagtggcttt
    14641 aacgaacaac gcagcgtccg caatgtagat agggccatca taagcctgaa cgcggccttt
    14701 attgctcttg ccgtcctgca gattttgctc aacaaaaacc acttgccagc tatccggcgg
    14761 cgtcgggaag gttttagtgt tgtacatcag caggttcggc ccccattggt aaggtgtgcc
    14821 gtaaactttg ccgccaacgt taaaccagtc gcctttaacc acgcgcggat caagcgtttt
    14881 ccagttggga atcaaggcgg tattaatcgg ctgcacgcgt ttacccataa tcaaacgcag
    14941 cgaggcatcg ccggatgccg taaccagatc gtaaccccct ttggtcatca gactgaccat
    15001 ctcatcggaa gtcgcggcgg ttttaacatt caccgcgcag cctgtctctt tttcgaattg
    15061 cgttacccag tcgtattgtt tatcagtttg tccgcgttcg atatatcccg gccaggcgat
    15121 aatatccagt cgcccttccg gtttatctaa attggtaggc ggttcggcgg cgtgagcggt
    15181 cattattgtc atgctgagcg cacacaggct gctgcgggca aatgtcttgc tcataaggtc
    15241 ttactcctgt cgtaattaat tgttcagccg ccgtgccgta aaaatatctc cttgtttaac
    15301 catagacggc ggcttgcagg cacaccttcg cccctgagaa aatttaatca ggttgtgagc
    15361 taccgcgcat tatgccgtct gtaaggttat cttctggagt atagacaagg agttaggaaa
    15421 tatgactgct attcgaaatt catattaccc aatctaagag gaattaaatt aaacgctatt
    15481 acagccgttc ttgaataagt ttgcctaatt gttttaccgc ctgttcttca cgttctcccc
    15541 acagccacgc ggtattaaaa cgaaaaaaac gtgaccagtt ttcaccggta gaaaacattt
    15601 tacctggcgc aatactgata tgatgcgtca gtgccgccag gctcaattcg ccggcatcta
    15661 acggctcggg gagctccagc cagagaaaat aaccgctgtc attatgatga attttcactt
    15721 ccgcaggcag ataacgcagc agtgcctgcc aggcacgttg tttacgttcc gcaagctggc
    15781 gacgcaggcg acgaagatgg gcgtcgtatc ggcgcgtgga aaggtaatcc accagcgcaa
    15841 gctgcatcgg tgagctggtg gaaagcgtac tcatcagctg caagcgttga attttacgtg
    15901 catgttttcc ggcggcgacc caaccaatac gaaaaccagg caccagacat ttcgaaaacg
    15961 aagagcaatg caaaacgcca tcgtggcgat cccacgcttt cgcaggcagc ggtttttccc
    16021 gtccaaaata aagttcgctg taaacgtcat cttcaatcag cgttacgttg tactgattga
    16081 gcaacgccac cagttgtgct tttttttgcg gcgttaaggt aaatccgagt ggattctggc
    16141 tattagtcat cagccagcac gctttcaccg gatactcctg caacgccagt tccagcgcct
    16201 gaagatcgat cccttcttta acatccgtcg ccaccgataa cgccttcagc cgtagccgct
    16261 ccagcgcctg caacgcaccg tagaaacaag gattctctac tatcacccaa tcgcccggtt
    16321 cagttaccgc ttgcaaactg aggtttaatg cctctaacgc cccggcagtg atgacaattt
    16381 catcaggaga aatggtgatc ccctgtaagg catagcgacg ggcaatagcc tgacgcagtt
    16441 ctgcgtttcc tggcggcaag ttttcaatca cgctcatcgc cgtggcggtt ttgcttacct
    16501 gcgccagcga gcggtttagt tgttggaggg ggaaaagtcg cgggtcggga aaggccgagg
    16561 caaacggaac gaccgacgga tcgcgactgg cctgcaacat atcaaaaata taagtgttga
    16621 tatcgactgc ttcatctcga gtgactggaa tgactggcac tttcggcatt tttattgcct
    16681 gtggcgcaac gtaataaccc gactgcggtc gtgcgataat atatccctga ctttcgagca
    16741 actgataggc atggctgaca gtcataaagc tcatgccaga aagcgccacc tggtcacgca
    16801 acgaaggtaa acgatcgccg ggttgccaga tacccgacgc aatctgctcg cgtaattgtt
    16861 ctgcaagctg ctggtatttt ttcattatcg aatcgtaatt atgtgcagat gattcggcag
    16921 tctatatcag ttgttaaaaa tgacaacttt tcgttaactg taattaaacc atcaccagcg
    16981 ataactcttt gccaagcgcc tttgccgcga gctggacggc gtcgattttt gtcgcatgat
    17041 gcaagttaaa taggcgagta atctcttgtt taggtttgcc aattcgcctg gctaactctt
    17101 gctgagtgat ttctgactgt aaaaaagcat ttaacagcaa taccttagag gcgacgctca
    17161 aaggtacttc aataaagtga tcatgactat ttaatggcga aggtaaaggg ataagctcgt
    17221 tatcttcaaa ataaaaatca aatgcggtca gtaaagcatc ttttgccgct tccatcgctt
    17281 cagcgacagt ttcgccctgc gtcaacgctt cagggatatc cacaaaagaa accatataac
    17341 cgccttccgg cgcgggtgta agagtgacgg gataacgcat aatttcgact gtctctcgca
    17401 tgtcattaac ctttttaatt ggcgattaac tcaaaccgag ttgtttcagg attgctttac
    17461 gcaatggttc tttaatctca tcgcaggggt gacgcggcat gacactgcgc ctcccatgaa
    17521 acctgagttt caaatggttg ctgccattcg ctacatcgac gccctgagat tcgagccaac
    17581 gtctgaactc gctttgtttc acatcgcctc cctgcggctg agtatatcaa caaagtaaac
    17641 attagtgttt acctccgtgt gtaatttgct cgaaaatagc actaaatcgc cgcaacggca
    17701 gaatgcaatg caaaatcagg aaggaagatt ccagaatttc tttgcgctaa tttgcaaaac
    17761 tgaataataa cctcaagaaa cgatcggcat catagcggca aaaggtaaga aaaaggtggc
    17821 aggatgttta cgaaggcgtt atcggttgtc ttattaacgt gtgcgctgtt ctcaggacaa
    17881 ctcatggcag ggcacaaagg acatgaattt gtgtgggtaa agaatgtgga tcatcagctg
    17941 cgtcatgaag cggacagcga tgaattgcgt gctgtggcgg aagagtcggc ggaaggtttg
    18001 cgcgagcatt tttactggca aaaatcgcgc aaaccagaag cgggacaacg ttgagtcgtt
    18061 tgatgccgga tgcggcgtaa cgccttaccc ggcaggcaat atcatgcaga ttcaataagt
    18121 tgtgagtaac cagaaaactg gcgttttacc cttttcgctt cggcaatgtt ttcagcagct
    18181 catccggact tacggatgcc actacgcttc ccggtaacgg cattttcagt atgtgatttt
    18241 tgattttgcc aatgacgtgc atctcgcatg ggcggcaatc aaactttagc gttaatactt
    18301 catcgccgtt aaccagttgc attggcgtcg ccttccagct tttgatattg cctttcgcct
    18361 gtttcgggca cagattaaag gcaaagcgca gacaatgctt ggtgatcatc accgggactt
    18421 cgcccttctc ttcatgtgcc tcatacgccg cgtcaatcag ctgcacacca tagcgatgat
    18481 aaaattcacg cgctttctgg ttgtaaacgt tcgcgaggaa actcagatgt gtttgcggat
    18541 aaaccggcgc aggatcagca accggtttac ggctgccgcg ctggtaactg gcaagacgcg
    18601 cagcatccag catgtcggca gcttcacggc ggaactggtt taacagactg tttggtacaa
    18661 acagcgcccc cggcaaatta atttgcacat cgcgggcgta atagagggtt tgccccagtt
    18721 ttgccagacc atccttcaga ttgttcattg ctttttctgc gttattggct tcgtcgaact
    18781 gcccatccag cgtatgcgtt atgctgaccc cctcttcact ggtgagagtc agaatcaatt
    18841 gttcctgcca accgcccagt tcaatgtcta ccgccacccg acgttcgcta gaggtttttg
    18901 tcagtgcctg ctgccagtta tgatcaagat tacggtttag tgggtgatgc gggcgaattt
    18961 tgtgcaaatc tgctggcatt tcattgggcc agacgcggta ctgattttct ccggttttct
    19021 cgaccgtatt ggcacgaaaa ccgacgactt cacgtttaat caacacgttc aggccatcgc
    19081 catttgccag tggctcggta acggcaacat cgagatgatc tttcgccact ttcactactt
    19141 cacctaccgg caggccgata aatttcggcg aatcgaacgc gccaatatcg cctttacggg
    19201 cattcacaaa ataatctgtg ctaccacggt ggaaagtctt ttccgtcgat ggaacaaaga
    19261 aatgttcagt acgacctgat gaagcgcgcg ccagatcgcc acgttcttca ataatggcat
    19321 caagcatctg gcgataatgg gcggtgatat tcttcacgta gctcatatct ttgtaacgcc
    19381 cttcaatctt gaaggaacgt acgccagcat caatcagcgc gccgaggttg gcagtctgat
    19441 cgttatcttt catcgacagc agatgttttt cataggaaac cacccgcccc tgatcgtctt
    19501 tcaatgtgta tggcaaacgg cacgcttgcg agcaatcgcc acggttggcg ctacgccctg
    19561 tttgcgcatg agaaatgtag cactgacccg aataggccac gcacagtgcg ccatgaataa
    19621 agaattcaat ggtcgcgtcc gtggcctggt gaatcgcgcg gatctgatca agattcagct
    19681 ctcgcgccag cacaatctgc gtgaagccaa catcagagag gaacttcgct ttttctactg
    19741 tacgaatgtc gcactgcgta ctggcgtgca gttcaatcgg cggaatatca agttccagaa
    19801 tccccatatc ctgaacaatc agcgcatcga caccggtctg gtagaggtca gtaatcagcc
    19861 gttgcgcggg ttccagctca tcatcatgca aaatggtgtt aagcgtgacg aaaatttttg
    19921 caccataacg atgggcaaac ggcaccagct cggcaatatc tttcaggcta ttactggcat
    19981 tatgacgggc accaaaacca gggccgccga tataaacagc atcggcaccg tgcaaaatag
    20041 cttcgcgggc aatggcggca tcgcgtgccg ggcttaacag ttcaagtcga tgagaagata
    20101 cggtcacatt cttatccagt agctgaaaaa tggcggctat tttagccaca agcgcgacgt
    20161 tgcgaaatag ttttcccgct taactgcgcg ggtaatggat gagggaatga aaatgtacgg
    20221 tttgctcccc accattacga tagatatgcg tgacgtctgc agcaaatcga acgccttccc
    20281 cacaattaag ggtctgccat tcaccatcaa cgcacagatc aagttgtcca tcaatgacga
    20341 cgacatgttc gattaccccc ttctcatggg gcgtcgattc gcttattgca ccgggtgcca
    20401 tctgaatcga gaagtgttca aagcagagtt gtggatcata aggaaacagc gaaatgatca
    20461 ccatcgcctg ctgttgtgga tcataaactg aaggcgtagc ggactgtggc ggagaaataa
    20521 atgtcgaaaa tggcacgttc aacccagtgg caattttcca taatgtcgcg accgtcgggc
    20581 tggactcatt acgctcaatc tgtcctaaca ttgctttaga aacgcccgtc gcttctgcca
    20641 gtcgcgaaag actccagccg cgctgctgac gtagttgttt aagtgtggtg gataaaaagc
    20701 gagcgagatt ttccatgcga ctcctccggc aaaacggaag tttatcactt gtgcgttata
    20761 acggacaaat gctacggtgc ctgtacgcta taacgcacga ggtgactatg cgtctgtttt
    20821 ctgttcctcc acccacgcta ctggcggggt ttctggcggt attaattggc tacgccagtt
    20881 cagcggcaat aatctggcaa gcagcgattg tcgccggagc caccactgca caaatctctg
    20941 gctggatgac ggcgctgggg ctggcaatgg gcgtcagtac gctgactctg acattatggt
    21001 atcgcgtacc tgttctcacc gcatggtcaa cgcctggcgc ggctttgttg gtcaccggat
    21061 tgcagggact aacacttaac gaagccatcg gcgtttttat tgtcaccaac gcgttaatag
    21121 tcctctgcgg cataacggga ctctttgctc gtctgatgcg cattattccg cactcgcttg
    21181 cggcagcaat gcttgccggg attttattac gctttggttt acaggcgttt gccagtctgg
    21241 acggtcaatt tacgttgtgt ggaagtatgt tgctggtatg gctggcaacc aaggccgttg
    21301 cgccgcgcta tgcggtaatt gccgcgatga ttattgggat cgtgatcgtt atcgcgcaag
    21361 gtgacgttgt cacaactgat gttgtcttta aacccgttct ccccacttat attacccctg
    21421 atttttcgtt tgctcacagc ctgagcgttg cactccccct ttttctggtg acgatggcat
    21481 cgcaaaacgc accgggtatc gcagcaatga aagcagccgg gtattcggct cctgtttcgc
    21541 cgttgattgt gtttactgga ttgctggcac tggttttttc ccctttcggc gtttattccg
    21601 tcggtattgc ggcaatcacc gcggctattt gccaaagccc ggaagcgcat ccggataaag
    21661 atcaacgttg gctggcagct gccgttgcag gcattttcta tttgctcgca ggtctgtttg
    21721 gtagtgccat taccgggatg atggctgccc tgcccgtaag ttggatccag atgctggcag
    21781 gtctggcgct gttgagtacc attggcggca gtttgtatca ggcgctgcat aatgagcgtg
    21841 agcgcgacgc ggcggtggtg gcatttctgg taacggcaag tggattgacg ctggtcggga
    21901 ttggttctgc gttttgggga ttaattgccg gaggcgtttg ttacgtggtg ttgaatttaa
    21961 tcgctgacag aaaccgataa taaaaagcgc catctaacga tggcgcgtct ggaactactt
    22021 tttgttaacg tcaaacatgg tggcatcggt cgccatgtca tcaataactt gtttaatatt
    22081 ttcgaaagcc attggcgtac tttcgttatt caggtcttta ccttcgccct gacgaacgac
    22141 tttgataacc ggtttattag tcgctgcatc aatcagctca ccttcaaaat acaagcgagt
    22201 atccatggta cggtggcctg tagccatttg cgtccccgca accactaatg caacaggaac
    22261 cacttcatag aattgcagcc cttctttgct ggtatctaca ccggtaatgg ccccacggaa
    22321 aatcagacta cgcggccccg cggtggtaac cagtggttta cgctgcgcta tcgcttcttt
    22381 catttcggtg ttggtatagt tcaaaatttt atccagaact ttctgcccta cctgggtcga
    22441 aggtttcggt accggataat aagtgattgg gttccagacg atgctgtcat atttgctttg
    22501 atcaaaactc gggtctaccc aacgtaaaac cggtttacct gtagccgagg ttgtttcttt
    22561 taaatcagag taattgttta aaaaaccaga atatttatct ggctgggtga ttttagaggc
    22621 acaaccagac agagccaaca agccgcataa agctgcaact ttcgcaaatg atgtggtacg
    22681 catgaatagt ttcctgagat gattatatta ctttttaagt tatagcaaaa agcgccgctt
    22741 cgtgttgtgg caaaaaaagt gttggtgaaa ataaattttt ttagcccctt ttcagacgac
    22801 atagtcaatg ccgcaaaagg ggctaagtca atcatcagta gttaaatttc agtaagttaa
    22861 gctgctaaaa aacttcgact actgtgatta atttcacatc ccttcgataa aaaatatgct
    22921 taccgaaaag aaggacagta aaccatctca gcgtggggaa ttttcacgcg ctttgttctt
    22981 accgggtcat tttttacgtg ccagcatcgt ggcgaaacgc agtttaatac gattaccgtt
    23041 ggcgtcggtg cggtgcagct cgccgacgtc ttcattgtat ttcaccatct cccagccttc
    23101 gtaatatcga cgtaattctc cctctttgaa ggcaaacggg aagccgacgg tacatggata
    23161 atcagcggta tccatcgccg ccacaatcag gttgtaaccg ccaggtttag tgcaacgttg
    23221 catattggca atcagccctg ggatggtttt agcctcaagg aacatcagca ccacagtcga
    23281 aagaataaaa tcgtactctc catcaaatgt gaggttattc agatcaacga cccgggtgtg
    23341 taaattatcc agattttcaa tggatttaat gcgctcgacg ttggcgatac tcatggcatt
    23401 tttatcccat gcgtcaacat cataaccatt ggctgccagg taaagactgt tacgaccatt
    23461 gccacagccc agatccagcg ttttacccgg tttaaccact ttcaccgctt ccagtacttc
    23521 agagtgtgtg cgggttaatt catatttatc agtaaaataa ttttcgtcac gaatgatcat
    23581 tctttgtcct ctgctttcat taaaacggcg cgctcggttc tgactaacaa ttttccctgc
    23641 atcagaagcg ccaaagtacg gatgagcagt attgcaataa taaaattggt aaagataaac
    23701 agcggcaccg ccagcgtatg gaaaaatcca ttatcgctgc cactccccag atgcaaaccg
    23761 gtggttgcca gtgcagatac gccgaacgag aaactccaga atgaagcatt aaatggctgg
    23821 gagagatacc atggcatcag acgtagcata aacagcagtt gcagcagtcc ataaccgaaa
    23881 agcattttcg ccagcgtgtc accctcgccg ccgttgacgc tcagccaggc actacaggcc
    23941 accagcgcag gagcgagctg aatgccgagt gatgtccgca gtgccgtggg taattctccc
    24001 gaactacgca gacgctgcaa gatcaccggt tcaaggctta gccatgagaa aacgcctgcg
    24061 cctaaaaaca ccagaccggc gtcggtgtag cccaacgcac cacaggccat tgcgctgata
    24121 aagttgttgg caactgtcgg cagatacagt ccaggcgtag tagcttcttc agggtgagat
    24181 ccgcgccata atcccgcagt ttgccaggcg gcataagcca actgaaccac gacaccaaaa
    24241 ctgaacaggc acaccgccag tgggcgaaac cacggaacaa aaccaatcgc caccagcatc
    24301 gtcgttgcag gaaacaaact cacaaaactg ctcagcactg gatggcgaac ttccgccagc
    24361 acgctatgcg gaaagcgtat gagtcgggta ataaatgcgc tagtcaataa tccccagatg
    24421 atcatcgcca gaatcaccag cccatcccct aaccagtggc tgacctgcca aacctggctg
    24481 gcatagcgcc aggcaaatcc catccctatc gtccccaaca caataccaaa gtagcctgcc
    24541 ggcaaattga gcactttatc gctctgcatc ttcttactca ttttgtttaa tttataaagt
    24601 atatttgaaa tgcattttat ggaattttct cgagcatagc cagagccgca gaatttgcta
    24661 cggttatagt aagtcacctg tgggaaaacg ctatgcgcaa atatcgtcta agtgaagaac
    24721 agcgagcctt tagttatcag gaagatggca ctaaaaaaaa tgtgttatta cggcagatta
    24781 tcgccattag cgattttaac gatgtgatag caggaaccgc tggcggctgg atcgatcgcg
    24841 aaacggtgtt agcacaggaa ggaaattgct ggatttacga ccagaatgct attgcgttcg
    24901 gcggaacggt gatttccggt aatacgcgca tcaccggcac cagcgtactg tggggagagg
    24961 tttacgcaac ggataatgtc tggatcgata atagtgaaat caaccagggt gcatatataa
    25021 gtgacagtgt caccattcat gactcactgg tttgcgggca atgtagaata tttggtcacg
    25081 ccttaattga tcaacattct atgatcgtcg ccgcacaagg cttaacgccc gaccatcaac
    25141 tcctgctcca aatttatgat cgcgcaaggg ttagcgcctc gcgtattgtc caccaggcac
    25201 aaatttatgg cgatgccgtc atccggtatg cttttattga acatcgcgcc gaagtttttg
    25261 attttgccag tattgaaggt aatgaagaga acaacgtctg gctatgcgat tgcgctaaag
    25321 tctacggtca cgcgcaggtg aaagctggca tagaagaaga tgccattcca acgattcatt
    25381 acagttcgca ggtggcggaa tatgccattg tggaaggtaa ttgtgtgtta aaacatcatg
    25441 tcttgattgg cggaaatgcc gtggtacgtg gcggaccgat tctgctcgac gagcacgttg
    25501 taatacaagg tgagagccgt attacaggtg cggtgataat tgaaaaccat gtggagttaa
    25561 ccgaccacgc cgtcgttgaa gcctttgatg gcgacacggt tcatgtccgt ggtccaaaag
    25621 tcattaacgg cgaggagcgc attacgcgaa cgccgctggc agggttatta tgaatcgata
    25681 atacgcgcgt ataagttcac atcatcatag gcatcattca ggaactcagc ctgtttcagg
    25741 caaccttcaa ggataaaacc attgcgcaaa gcgacctggt tgctttgcgg attgtccacc
    25801 cgacatttga tcacgaagcg tctaagttca ccagactgag cgtaatgatg aatcagtgcc
    25861 tgcagcgcct gagaaatgat cccctgcccc tgatgagatt cgtccagtca gtagccgatt
    25921 tcagcggttt tattcagtgg ttcaatacga ttaaacgaga taacgccgat aagttcatct
    25981 tctttgaaaa tcatgaacat tttggcatag ccgcgttgat gcaacatcac attaccctgc
    26041 accgtttttc gcgtgtcctc ttcaatctga acgaactgcg gccagtttag cgactgctgt
    26101 aaccaggttt tatttttaca gattaactga taaagaggag tgacgtgatt ttctgcaaca
    26161 gcatgtaatt caagtgattc gcttactttt atcgtttcag tcatcttatc tccagtcaat
    26221 ccatgcagtt gttaaaaaag ccgcctggct ggcggcttag gaaagtttac acctctttga
    26281 cgctctcatg ctgcaggatt ttgagcatct catcttttaa ctgtagcttt tgttttttca
    26341 tgcggaccac ttccgcattg taccctcgat cgtcggaacc ttcctttctg gcaatttcat
    26401 gatcaagttt attgtgttta tcgaacaagg acataaagcg aggattttcg tttttcagac
    26461 gggatattaa atctcgatat tctggaaaca tacgcactcc ctgttagtgg ctgacaaggt
    26521 gtatctgaaa tacacaccat cagattactc atttaactta gggaaaaatg cgagcgggat
    26581 cgtgtttaac cagacggcac ctcccgaaga aggtgccgaa gaaaaaacga ttaactcatc
    26641 acgcggtcgt caacatactg acgtttatcc ggcgctggcg ggaaatactg atacagccat
    26701 gtttcactga tagcgtcgcc ctgacaacgc agatacatcc gcatatcgac cggatcagtg
    26761 gagtccgaag tcggatacca gtcaaactga atacgataac catcaatggg ttcaatatag
    26821 agaatttcga tttgcttcgc ttccccactg gaaagcgtaa tcaccggctc aatgcctttt
    26881 ggtgcggcag ctttcagatc accaccaacg aaatcgacgg caaaacgacg cgcccatttt
    26941 tcgggatagt gttcacctgg tgcccaacct tccgggaaac cgcccatgcc ggtacgcgtc
    27001 gccataacgc gcgctaatgg gcaatgaaca ggcggttgcg cactccagta cagacgatac
    27061 tggaatgcaa actcatcacc cgcctttaca gctttttctg gctgccagaa gcagacaatg
    27121 ttatccagcg tttcgcccgt tgttgggatt tccatcaggc cgatggtgcc cttaccccac
    27181 ttgttacgcg gttccaccca cagacttggg cgtttgttat accagcccat aatgtcctga
    27241 taatgggaga aatcacgatc cagttgcaat aaaccaaacc ctttcgggtt gttgtcggtg
    27301 taagcattga actgcaattt ttgcggatta ttcagcggac ggcaaatcca ctcgccgttg
    27361 ccccgccaca tggacagacg atcagagtca tgaatttgcg gatgaatggt gtcgcacatc
    27421 cgacgttcat tagtaccgca gctgaacata ctggtcatcg gcgcaatgcc cagctgttta
    27481 atgtctttgc gcgcatacag gtgattttcc acatccataa tcacctgact tttctcacaa
    27541 tggatagtga acttataggc accagtaatg ctggcgctat cgagcaacgc ataaacggta
    27601 aaggtagttg cccccggttt taccgtatca aaccagaagg cggtaaagtc ggggaactct
    27661 tctttactgt cggtgtaagt gtcaatcgcc aggccgcggg ccgacaaacc gtattgatat
    27721 gtgtcatcaa cggcgcggaa ataactcgct ccgaggaatg atactacatc acggcgcgcc
    27781 agttcggggg ctttaaacac gcgaaaaccg gcaaagccga gatcgctttg cccttctaat
    27841 tgtttggtat caacacctgc atcgttgtat ttgaacaact ccgggcgaaa gtgaatttca
    27901 cgcgccagat gtgttgctgg atctacagaa aacatacgaa cgcggcgacg gaatcccatt
    27961 cccatatgga agaactgagc gtccagttga cggttctcaa cgttatgcca gagcgatttt
    28021 tcggcgtcgt attgaatact gttataagcc tgcggcgtca ttgtcgccag cgtgtcaggt
    28081 aacggacgag gcgcaccacg ccacgctgtt tgcgctaagt cgtgcgccat tgactgtaga
    28141 atggagaagt caaaacgctg ggtttgcccg tcggcaatat cagaatctgc cgcgaatgcc
    28201 gcctgagaaa aaagagaagc aatgccgctg gtaccgcaca cggcggccat agccattgaa
    28261 cctttaataa atcgtctacg atccatacct gaaagtgagt ccttctggtg ttgtgaatgg
    28321 tcccgcgctg gtcgttttca gcggcaagaa agtgcttatt tatgaccgct cactctagac
    28381 aatatttatt aataatccaa ttgttgaact gagagaatat gcataaatcg gcaatttttt
    28441 tatgtcgatg ggatgagcct gattcgtttg taaaatcagg caaatttttg caaaggcgga
    28501 tggtcagaat ttaaagcaaa ccacagcgac gtttactgcg ttcttccgct tcctgataaa
    28561 gctggaactc atccaaaacc ggacaaccca acgcctgctc aaatgcttcg cgactggcat
    28621 taccgtggct gcgtgcctgc gtttcattac ccagatttga ctggaagatc cccgccgcgc
    28681 taacgggcaa gaaatcttca taggtaatgg gttgcgccgc gacccaacca cgttcaatta
    28741 agggctgtgg atcgtctccg ggatgaatcg cctgacgatg cgcctcacct gaaggcgtca
    28801 gacggtaccg gaaccatgcc agcccttgct gacgcattaa aaactcactg tcaggaaaag
    28861 tgcggaaggt ttcctgtaaa tgcatttggt gagtgagatt atcctgcccg gttccagcgt
    28921 tacgcagaag atcatcatac agttgtcgac ctttcggcgt taatgccacg ccacgctgct
    28981 caatttcacc aaagcgcgca gtatgcgtgc cctgtttctg ccccgcaaac aacaccgtct
    29041 cttccagtgc tttaaagctg gtctggcgta gtaaaatcgg tacctcgcgg cgcggcggcc
    29101 cttcgatcag aatttttggt tcaattccgc attcaggcat catcgactgc acccggtcaa
    29161 tatccagcgt acgtggcgtc agatggttga tatggcatcc aggaaaacag accacatcag
    29221 caatcaaccg atgttcgttg tgcaaggcgc gataggtttc ttcatctacc gttgctgact
    29281 ggtgccaacg gaacgtttcc agggcctcct gcacaaactc ctgtgcctgt gtttcgttaa
    29341 aaccgccccg ctggtcatac tcctctaaca gttgtcgaca acgtggggtg aagatatcgc
    29401 gctgacgcag aatctccgcc gctttctggc gcaaaatttc gttctcgata agctcaaggc
    29461 ggagtaagga ggtaaaaacg cggaagggat tacgcgccag agaagcatca tcaatgggcc
    29521 gaaatgctgt cgaatgtacc ggcacccctg cctgcgagag atcgtaatag ctcaccgggt
    29581 acatccccat aatggcaaac atccgccgaa gagtagcaag ctcttgtgca gtcccaacgc
    29641 gaatcgcccc atgacgttca acattcagtc gcgccagctc gtctgcattt accatttttt
    29701 cgtgcagttg aggattgttt tccagcacag ccagattcac atcagctacc agttccagca
    29761 gcgtgccata ttgcggaact tcttgctggt acatggctga cattgcctgc gaaaactgtt
    29821 cccgaatctc atccgccgtg atgctgttcg ccatagtgtc tgcctccagt ggtcagtaat
    29881 ctggagtgta ggtaaccgca ttcactctgg cgggaagaat ttacaaactg tgatctcgcc
    29941 gcgaaaacat caatattatc cattttgctg taacataatt gctttaattg ttaataatat
    30001 tttgcaatca agttatcata atcaaacaac ttcacttgtc agcgacaccg cttcgttttt
    30061 aacatcgctt atggaaaaaa atagtctgtt tagtcagcgc atccgtttgc gccaccttca
    30121 tacattcgta gctgtcgcac aacaaggaac tttggggcgc gcggctgaaa cccttaattt
    30181 gagtcaacct gcgctctcta agacattaaa tgaactggag cagctgacgg gcgctcgctt
    30241 gtttgagcgt ggtcgtcagg gggcgcaact taccttaccc ggcgaacaat ttttaacgca
    30301 tgcagtcaga gtacttgacg ccatcaacac tgccggacag tcgcttcatc gtaaagaagg
    30361 tcctaataat gatgtcgtca gggttggtgc actacctact gcggcactgg ggatattacc
    30421 ttcggttata ggtcagtttc atcagcaaca aaaagagacg accttgcaag ttgcgacaat
    30481 gagtaaccct atgattctgg cgggtttgaa aaccggggaa atcgatatcg gcattggtcg
    30541 gatgtcagat cctgaactga tgaccgggct taattacgaa ctgctgtttc ttgaatcgct
    30601 gaagctggtt gtccgcccta atcatccgct acttcaggag aacgtaacgc taagccgggt
    30661 gctggaatgg ccggtcgttg tatcaccaga aggcactgcg ccacgccagc attcagatgc
    30721 attagtacag agccagggat gtaaaattcc ttcgggttgt atcgaaacgc tgtctgcttc
    30781 gctatctcgt caacttacgg ttgaatacga ctacgtgtgg tttgtccctt ctggcgcggt
    30841 aaaagacgac ctgcgtcatg ccacgctggt ggccctgcct gttccgggac atggtgcagg
    30901 cgaaccgatt ggaatactga cccgcgtaga tgcgacgttc tcttctggtt gccagttgat
    30961 gattaacgct attcgaaaat caatgccgtt ctgaaaggtg aagggatctg tcgatccctc
    31021 ctttaacatt ttcacaccgg agcgaaacta actggttcac ccgctccgcg aggttctgcc
    31081 aacacagatt gtttgttcag acggaatacg tccaccgcct cagttaatcg tgccgcctgt
    31141 tcttcaaggg acaccgccgc cgctgaggcc tcttctacca gagaagcatt ctgttgcgtc
    31201 accttatcca tttcagaaat cgcctggcta acctgcgtta tgcccctact ttgctcatcc
    31261 gaggcggcgg caatttcctg catgatatgt gtgacactcg cgacggcatc aacaatagtg
    31321 ctcatggttt tcccggccgt tgccacctca tccgatccca ggtcaattaa cctgactgat
    31381 tcactgatca agccttcaat ttcttttgcc gcctgggcgc tgcggcttgc gagtgtccgt
    31441 acttcgctgg cgacaacggc aaatccacgc ccttgctcac ccgctcgcgc agcttcaacg
    31501 gcagcattca gtgccagaat attcgtctgg aaagcaatac tgttgatgac ggcggtgatc
    31561 tcagaaattt tcttcgaact cgtggagata gcgcccatcg tttttactac accggaaacc
    31621 gtctgcccgc catcgctggc tttaatagaa gcctcttgcg ccagtttgct ggcataatgc
    31681 gcgttatccg cattctgttt caccgtcgca gtgagttgct ccatgctggc ggcagtttgt
    31741 tcgatagccg ccgcttgttc ttcggtgcga gatgacaggt ccgcattgcc agctgaaatt
    31801 tcgctggtgc cacgataaat ctcttccgca ccctgtcgaa cagtccctac ggtcatcccc
    31861 agtgaatgct gcatttgctg taaatgacga cttaagcgac cgatttcatt acgacccgcc
    31921 ggttcatcct tcatcgtcag atcgccacta gcgatttttt caatccgttg tgcggcatgt
    31981 tgcagtggac gaatgacgat ccgacgtagc accataaatg ttatcagcgt catgaccagg
    32041 gcaagcacaa acgcaccaat catgaacatc ccgcccaggc gggtgcgctg atgggccagt
    32101 tccgccagtt ggttggctct ggtgctacgt atcttaacgg ctttgttcaa aatatcggta
    32161 taagcattat ccagcgttcg gatctgctca ctttcatgat tgataatcgc ttcaaacatg
    32221 ccatttttgg catatttcaa cataggttgc atacccgtga tataagcctg aaagcgttga
    32281 tttaattcag tgtcgagggc ttcatcagca ggtgttttca ccggccgatt ctgataagca
    32341 cgataacctt gctgcgactg tttaatctcc gattcggctt gcgcaatatt tcgcttcatt
    32401 gcttccattt ccgcaatacg actcgcagcc ccggcctgaa tcatattgat ccgggctgaa
    32461 cgcaaaaaat ctgaactgtt cgctaacccg gtccgaatct caatctccgc agtaacatca
    32521 cgctgatcgc gatcggcctg ccagaggaaa tagccagcca gggctgaact taatgcgaat
    32581 agaactaaga taccgcctag aatgcaggca aataacggaa ccaacctgat gtgatgcaaa
    32641 aaacctaatc gctgtgaggg agttgtattc attaccgttg tctctcgtcc aggtttactc
    32701 ctgatagaaa gtcatcggca tttgacggta attacttacg actaaaagcc tctttgcgtc
    32761 acatttttac aacaattatt gcagagaaga ttaatgccat tggaatgaag agagtaacga
    32821 atggttaaac tggctaaccc gaatgacgaa aaattctgga aaacagcacg caatttcgtc
    32881 aggtgattat tttcgtccct aaggaaacca cataggatgc tcacgggtgc ctgagacttt
    32941 ctgttttcag gctaaccggg aatcagaaag acgaaggccc cgaccgatat ttctatcaac
    33001 cgaggccaac gaatgaacca agcaattcag atgttagcct cttacccgcc atccggcaag
    33061 gagaaaggct atgaagcgca accctctggt ggtgtgtctg ctcattatct gcattacgat
    33121 tctgacattc acactcctga cccgacaaac gctctacgaa ctgcggttcc gggacagtga
    33181 taaggaggtt gctgcgctca tggcctgcac gtccaggtaa gggcaagcgc ggggattttc
    33241 cccgcgcatc tgactagcag attcattttc tcatggcacc ccttttacac ttcagttagt
    33301 gcatgttttt ttcgatgcac taatagaacc atcattacag acaaatttgc cgcctgccgt
    33361 acagtgtgag atcccccctt tcgaaccaga acagggtttg cgcccggcat cggcataaaa
    33421 ggaaacaagc gctcccataa acaaaataag tgccagtttt ttcatataac ctccatggtt
    33481 tatcgaatct tatgttcgat ttgaacgtta tacgctggtt tattttgata ccgttgatga
    33541 gatcaaagat cgggtgaatt gattaaaaat aagccagatg ga
//
LOCUS       DAZKIP010000041        32387 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZKIP010000041 DAZKIP010000000
VERSION     DAZKIP010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.041"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..183)
                     /locus_tag="ACHOUK_002194"
     CDS             complement(<1..183)
                     /locus_tag="ACHOUK_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9897645.1"
                     /translation="MGNIHGISCTANNYLKQAWNDIKNEYEKNKTYSITLFENTLVCF
                     MRLYKEIRRQQAEDYIP"
     gene            443..1363
                     /gene="cbpA"
                     /locus_tag="ACHOUK_002195"
     CDS             443..1363
                     /gene="cbpA"
                     /locus_tag="ACHOUK_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFP9897646.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFSRQFQHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            1363..1668
                     /gene="cbpM"
                     /locus_tag="ACHOUK_002196"
     CDS             1363..1668
                     /gene="cbpM"
                     /locus_tag="ACHOUK_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFP9897647.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(1822..2421)
                     /gene="torD"
                     /locus_tag="ACHOUK_002197"
     CDS             complement(1822..2421)
                     /gene="torD"
                     /locus_tag="ACHOUK_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFP9897648.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVDELENRVATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPTDHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LRQWLPEFVARCHQYDSFGFYAALSQLLLVLVEGDHQNR"
     gene            complement(2418..4964)
                     /gene="torA"
                     /locus_tag="ACHOUK_002198"
     CDS             complement(2418..4964)
                     /gene="torA"
                     /locus_tag="ACHOUK_002198"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFP9897649.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTSRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQTYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWHDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKCNGTVEQVTAFNGPVEMVAQCEYVPASQVKL"
     gene            complement(4964..6136)
                     /gene="torC"
                     /locus_tag="ACHOUK_002199"
     CDS             complement(4964..6136)
                     /gene="torC"
                     /locus_tag="ACHOUK_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFP9897650.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRAGANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            6266..6958
                     /gene="torR"
                     /locus_tag="ACHOUK_002200"
     CDS             6266..6958
                     /gene="torR"
                     /locus_tag="ACHOUK_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFP9897651.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPYTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            complement(6931..7959)
                     /gene="torT"
                     /locus_tag="ACHOUK_002201"
     CDS             complement(6931..7959)
                     /gene="torT"
                     /locus_tag="ACHOUK_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFP9897652.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            8042..10786
                     /gene="torS"
                     /locus_tag="ACHOUK_002202"
     CDS             8042..10786
                     /gene="torS"
                     /locus_tag="ACHOUK_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFP9897653.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQRLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDVPTALMGDPRRIRQVI
                     TNLLSNALRFTDEGQIVLRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFIQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAIGNAAQALETLQNSEPFAAALVDFDLPDVN
                     GITLARQLARQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQANNDLPLDVSQLNEDAQLMGTEKIHEWLALFTQHALPLLDEIDIARATQDSEKIK
                     RAAHQLKSSCSSLGMRSASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLNKKDLNAI
                     "
     gene            10858..11931
                     /gene="yccM"
                     /locus_tag="ACHOUK_002203"
     CDS             10858..11931
                     /gene="yccM"
                     /locus_tag="ACHOUK_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFP9897654.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWHNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETAGSTIFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGALGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFTVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(11980..12114)
                     /locus_tag="ACHOUK_002204"
     CDS             complement(11980..12114)
                     /locus_tag="ACHOUK_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFP9897655.1"
                     /translation="MADFIAQKIAELNKNTGKEVSEIRFTAREKMTGLESYDVKIKIM
                     "
     gene            complement(12142..12372)
                     /gene="ymcE"
                     /locus_tag="ACHOUK_002205"
     CDS             complement(12142..12372)
                     /gene="ymcE"
                     /locus_tag="ACHOUK_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFP9897656.1"
                     /translation="MHRWISQNNIRLPCGAFFISVLFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            complement(12347..12535)
                     /gene="ymcF"
                     /locus_tag="ACHOUK_002206"
     CDS             complement(12347..12535)
                     /gene="ymcF"
                     /locus_tag="ACHOUK_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP9897657.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            complement(12546..12758)
                     /gene="cspG"
                     /locus_tag="ACHOUK_002207"
     CDS             complement(12546..12758)
                     /gene="cspG"
                     /locus_tag="ACHOUK_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFP9897658.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            13044..13256
                     /gene="cspH"
                     /locus_tag="ACHOUK_002208"
     CDS             13044..13256
                     /gene="cspH"
                     /locus_tag="ACHOUK_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFP9897659.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            13698..14003
                     /locus_tag="ACHOUK_002209"
     CDS             13698..14003
                     /locus_tag="ACHOUK_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9897660.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            14110..14754
                     /gene="gfcB"
                     /locus_tag="ACHOUK_002210"
     CDS             14110..14754
                     /gene="gfcB"
                     /locus_tag="ACHOUK_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFP9897661.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLHIAGRDV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            14751..15497
                     /gene="gfcC"
                     /locus_tag="ACHOUK_002211"
     CDS             14751..15497
                     /gene="gfcC"
                     /locus_tag="ACHOUK_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFP9897662.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VAQLVTQPQLRDRLWWPGALLTDSAVKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGNYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVIVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            15497..17593
                     /gene="gfcD"
                     /locus_tag="ACHOUK_002212"
     CDS             15497..17593
                     /gene="gfcD"
                     /locus_tag="ACHOUK_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFP9897663.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEPLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            17639..18778
                     /gene="gfcE"
                     /locus_tag="ACHOUK_002213"
     CDS             17639..18778
                     /gene="gfcE"
                     /locus_tag="ACHOUK_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFP9897664.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            18766..19212
                     /gene="etp"
                     /locus_tag="ACHOUK_002214"
                     /gene_synonym="yccY"
     CDS             18766..19212
                     /gene="etp"
                     /locus_tag="ACHOUK_002214"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFP9897665.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            19232..21412
                     /gene="etk"
                     /locus_tag="ACHOUK_002215"
     CDS             19232..21412
                     /gene="etk"
                     /locus_tag="ACHOUK_002215"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFP9897666.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDMVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(21532..22836)
                     /gene="appA"
                     /locus_tag="ACHOUK_002216"
     CDS             complement(21532..22836)
                     /gene="appA"
                     /locus_tag="ACHOUK_002216"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFP9897667.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPEPELKLESVVIVSRHGVR
                     APTKATQLMQDVTPDAWPNWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLTKKGCP
                     QPGQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLD
                     NANVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQAL
                     PSELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNA
                     QFYLLQRTPEVARSRATPLLDLIMIALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANL
                     GGALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSL
                     NTPPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(22916..23008)
                     /gene="cbdX"
                     /locus_tag="ACHOUK_002217"
     CDS             complement(22916..23008)
                     /gene="cbdX"
                     /locus_tag="ACHOUK_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFP9897668.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(23021..24157)
                     /gene="appB"
                     /locus_tag="ACHOUK_002218"
     CDS             complement(23021..24157)
                     /gene="appB"
                     /locus_tag="ACHOUK_002218"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFP9897669.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARDDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLSTKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(24169..25713)
                     /gene="appC"
                     /locus_tag="ACHOUK_002219"
     CDS             complement(24169..25713)
                     /gene="appC"
                     /locus_tag="ACHOUK_002219"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFP9897670.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPVLLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(25847..26704)
                     /gene="hyaF"
                     /locus_tag="ACHOUK_002220"
     CDS             complement(25847..26704)
                     /gene="hyaF"
                     /locus_tag="ACHOUK_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFP9897671.1"
                     /translation="MSETFFHLLGPRTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSQRQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(26701..27099)
                     /gene="hyaE"
                     /locus_tag="ACHOUK_002221"
     CDS             complement(26701..27099)
                     /gene="hyaE"
                     /locus_tag="ACHOUK_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFP9897672.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            complement(27096..27683)
                     /gene="hyaD"
                     /locus_tag="ACHOUK_002222"
     CDS             complement(27096..27683)
                     /gene="hyaD"
                     /locus_tag="ACHOUK_002222"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFP9897673.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPESVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            complement(27680..28387)
                     /gene="hyaC"
                     /locus_tag="ACHOUK_002223"
     CDS             complement(27680..28387)
                     /gene="hyaC"
                     /locus_tag="ACHOUK_002223"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFP9897674.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            complement(28406..30199)
                     /gene="hyaB"
                     /locus_tag="ACHOUK_002224"
     CDS             complement(28406..30199)
                     /gene="hyaB"
                     /locus_tag="ACHOUK_002224"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFP9897675.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPTTWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(30196..31314)
                     /gene="hyaA"
                     /locus_tag="ACHOUK_002225"
     CDS             complement(30196..31314)
                     /gene="hyaA"
                     /locus_tag="ACHOUK_002225"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFP9897676.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATSIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            31740..31827
                     /locus_tag="ACHOUK_002226"
     tRNA            31740..31827
                     /locus_tag="ACHOUK_002226"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31774..31776,aa:Ser,seq:tga)
BASE COUNT         7916 a         8528 c         8253 g         7690 t
ORIGIN      
        1 tggaatgtag tcttcagctt gttgacgtct gatttcttta tacaaccgca taaaacacac
       61 cagtgtgttt tcaaaaagcg tgattgaata tgttttattt ttttcgtact cattttttat
      121 atcgttccag gcttgcttca aataattatt tgcagtgcaa ctaattccat gtatattacc
      181 catatatagc gcctataaaa tttaataaat aataacgcac tagtaaaact taaagtgcct
      241 gtttcaacta tcaaaaatcg ctcacccttt ttcacctgtt taaaatatgt tcagcgcccc
      301 accttgaggc gacctcctct ccgcgatgat ttcaataaca tattctgtgt tggcatatga
      361 aattttgagg attaccctac acttatagga gttaccttac aggggttcct tcaatttgtg
      421 ttgatttacg cgagataacg ctatggaatt aaaggattat tacgccatca tgggcgtgaa
      481 accgacggac gatctcaaga caatcaagac cgcctatcgt cgacttgccc gcaaatacca
      541 tcctgatgtc agcaaagaac cggatgccga agcccgcttc aaagaggtcg ctgaagcctg
      601 ggaagtgtta agtgatgaac aacgtcgcgc tgagtatgat cagatgtggc aacatcgcaa
      661 cgatccgcaa tttagccgtc agttccagca tggcgacggt cagagtttta acgccgaaga
      721 ttttgacgat atcttctcgt caattttcgg tcagcatgcc cgccagagcc gtcaacgccc
      781 cgccgcacgc ggccacgata ttgaaatcga agtggcggta ttcctcgaag aaacgcttac
      841 tgagcataag cgtaccatca gctataacct gccggtttat aacgcctttg gcatgatcga
      901 acaggaaatc ccgaaaacgc tgaatgtgaa gatcccggca ggcgtcggca atggtcaacg
      961 catccgcctg aaaggccagg ggacgccggg cgaaaacggc ggtccaaatg gcgatttgtg
     1021 gctggtgatt catattgcgc cacatccgct gtttgatatt gtcggccagg atctggaaat
     1081 tgtggtgccg gttagcccgt gggaagcggc gctgggtgct aaagtcaccg ttccaacact
     1141 gaaagaaagc attttgctga ctatcccgcc tggcagccag gccgggcaac gattgcgcgt
     1201 taaaggcaaa ggtctggtga gcaaaaaaca gaccggcgat ctgtatgcgg tactgaaaat
     1261 cgtgatgccg ccgaaaccgg atgaaaacac tgccgcgctg tggcagcaac tggcagacgc
     1321 ccagtcgtct tttgatccac gtaaagattg ggggaaagca taatggctaa tgttacggtg
     1381 acttttacta ttaccgaatt ttgcctgcat accggcatct ctgaagagga attgaatgaa
     1441 attgtcggtt tgggggtggt tgaaccgcgt gagatccagg aaacaacctg ggtatttgac
     1501 gaccatgccg ccatcgtggt gcaacgcgcg gtgcgcctgc gtcatgaact ggctctggac
     1561 tggccgggga tcgcggtggc gctgacgtta atggatgata ttgcgcacct gaagcaggaa
     1621 aaccgcctgc tgcgccagcg gctttcccgg tttgtagctc atccgtgatg tgtgtttgcc
     1681 ggatgtgatg acgcatctta gcctgcctgc aaagagtact tttcctgtgg gccggataag
     1741 gcgtgttcac gccgcatccg gcggcggtgc cctaatgcct gatgcgacgc taaacgcgtc
     1801 ttatcaggct aacaaacgac gttatctgtt ttggtggtcg ccctcaacta acaccagcaa
     1861 taactggctt agtgccgcgt aaaaaccaaa gctgtcatac tgatggcaac gcgcaacaaa
     1921 ctctggtaac cattgccgca acgccgtgag tgttttttgc cgtaaactgt cgattcttcg
     1981 cgcaggaacc gtcccctctc ccagcgaaaa atgcagatga ctcaacaact cgagatagat
     2041 cgccagatga tctgtcggtt cgttgaaatt gccgctggtt tccatccctg cctcaactaa
     2101 caagcgttta atctcttgct cgtcctgttt gtaggccgat gcatacggca gcgccgcttg
     2161 tttgtcagtc atcagaaaca ggccgcaaaa gtcagcggcc agttccagac gggcatcgtc
     2221 acgtactgtc agagtggcaa cacggttttc cagctcatcc accgccgcag tgagcggcgg
     2281 ttcgcttttc agcaacgaaa accattcagc catctgcgca ctggcgattt gcgtcagttg
     2341 ttcatcgtcc agttcacggg agaacaactg cgccagccag gcgtaaacac aggctatctg
     2401 ttgtgctgtc agcgtggtca taatttcacc tgcgacgcgg gaacatattc gcactgcgcc
     2461 accatctcca cggggccgtt aaacgccgtt acctgctcca ctgttccgtt gcacttctca
     2521 atttccacca gcgtagtgtg cgcactggtc gcctgcgcca gctgcgaagt accgatgtca
     2581 atggtcaaca cgttggggtt accgtatttg cacagcgcac caggctcgcc gcctttatct
     2641 ggatcgtgcc atgccccttc gtgaattcgc gccacgccgg gtgcatagcg gtcagaaact
     2701 actgcccctg ccaacacctg accgcgagcg ttaaagacgc gtaccacatc accgttacga
     2761 ataccgcgcg cgctggcatc ctgcgggtta atgaacactg gctctttacc cgctaccgta
     2821 tattgctgac gcagagtttc cgactcacat aactgcgagt gaagtcggaa atccggatgc
     2881 acagattgca gatgcaacgg atacgtctgc gagccgggcc caccgtggga gcgttcgatt
     2941 ttctcaaacc acatcggatg cccctgacaa tcgtcgtagt tcatatcggc gatggttttc
     3001 gaatagatct caatcaggcc actcggcgtg cccagcggtt cgagatctgg atcttcgcgg
     3061 aatgcctggt ggcgaacaaa catctgcgga tggtcaaact cgacgtactc tttgttattc
     3121 cagaagtcat caaacgctgg caaatgaacg ccgcgtcctt tgccttgctg aacaccttcc
     3181 tgccagatgc gtttcagcca gcccatttcg tccagccctt cggtaaaggc ttcttcgcga
     3241 ttaaagcggc ggcacagctc gcggaaaata tcaaagtcgt tgcgcgcctc gaactgcggc
     3301 ggcacgacct gtttcatggc gataatgcca cggttggagt ggttgccgta ctggtcgaga
     3361 tcgttacgct caaactgcgt ggtcgcaggc agcacgatat cggcaaagcg gcaggttgag
     3421 gtccactggt tatctatggc gataaccgtt tccagtttgc gccagccttc aataatgcgg
     3481 ttgatctgct gatggcgatg gaaggggtta gttccggcaa aaatacacat tttcagcggc
     3541 ggcagtttta ccgatttacc gttccagttg attactttcc ccggttcgag gatcgcatcg
     3601 ataaaacggg caatcggaat ggtgctgctg tagcctttgt aatcactgtt gtcgtgaaca
     3661 ggcggaatcg acgtagagcc ggagaaacca ctcagaataa cgcctttacg ccccggcgtg
     3721 cctgcgccgt tatagtgcca gccaaaacca aagccaccac ctggcaggcc aatttgcccc
     3781 agcatcgccg ccagaaccac aatcatccac gcccactgtt caccgtgctg catacgctgt
     3841 acgcaccagc cagcaataat ctgcgttctg ttcgccgcca tctgccgcgc cagtccacga
     3901 atggtttcgg catcaatgcc ggtcagtttt tcagcccatg cggcatcttt cggctgaccg
     3961 tctttctcac ccagcagata cggcaggaac tgctcaaaac ccacacagta gttagcgagg
     4021 aagtttttgt cgtacaggtt ttcactgtac aacgtatgcg ccagcgccag ttgcagcggc
     4081 acgtcagttt gcgggttaac cgcaatgtgc ttcacatgct cgcgccccag atactcatgg
     4141 gtggatgtaa caaccggatc gatgctaatg acctcaattt caccggcggc gactttcgct
     4201 ttcaactgcg cgtaatattc ataaacatcg tgatccgggc accaccagtt cgcttgctgg
     4261 tttttcagca aatcggagcc ccacagcaca atggttttgc tgttctgcaa taccagcggc
     4321 caggaggttt gctgttcata cacttccatc gaaccgacta cgcgcggcag gatcacctgc
     4381 gcagcaccag tagagtaatc tccgcccgta ccaacgctat taccatgcaa ggcaatagct
     4441 ttcgccagca tccccgaagc gttatggaac atccccgtcg attgccaacc actggcggtc
     4501 agcaaggcac tcggcccgtg agttttctgt acgcgttcca gttcttcata gaacatgtcg
     4561 agggcttcat cccagctcac acgcacaaaa cggttatcac cgcgctggga ggtgtcgctc
     4621 agatggcgct tacgcagcca gtccacgcgt accatcggat aacgaatacg cgccgcgttg
     4681 tgtacatgat ccggcaatcc ggcaatcatt ttcgacggat atttatccag ttcgaacggt
     4741 tttgccgcca caaagcgacc atccttcacc gtcgcgcgga tagcccccca gtgcgacccg
     4801 gtaagaatgc cctctttcga gatgacagcc tcagtcgccg cttgcgccgc agtcgcacgg
     4861 cgcgacgtta acaatgacgg ccccagcatc ccggcgacgg ttaagccgcc gagttgtgcc
     4921 agaaaacgcc gacgtgatgc ctgaaagaga tcgttattgt tcattatttt tcttccttct
     4981 tatcgccgtg agccttacct gcggtgtcag acgcattcat ttgcagatat ttcaacaagg
     5041 tgcgttcttc acgtttatcg aggctggtaa agccgatcat gccgttgagc gtgccaatcc
     5101 agccgttagc gtcaaagtga gagatttccg gtgcgccgtg gcactggtta caggtgccgt
     5161 tgtacaacga atccgcataa gcccagatcg gtttgatatc gttcaccatg tcacctttct
     5221 tcatccacgc ggtggcctgc aacttgctcc actcggtatt ggtgtcggca acggtggttt
     5281 tctccagcgt ttttacctgc tgctgcacat caccacgaat cgaggcaaca aagatgcgtt
     5341 tacctgggaa ttgggtgagt acacgctgac gtccggcgct ttctgtccag ccggtaattt
     5401 caatttgcag ccagtcgccg tcacgtttaa ggactttcac ttccgaggca ggcagcagag
     5461 aaccagaggc ttctttatcg cctttcgccg cataaattgg cttaatatca atagagtaca
     5521 gcgtgtcacc actgtcatta gcaccggcgc gcagctcatc gaactgctta cggaagccgc
     5581 tactcatatc cggtaactgg tgggcaatac ctttatgaca gtcgatgcag gattgattat
     5641 ctttcgctgc caccttcatc tgacgtgccg cttcaggatg ctgcttcgca tgatccatcg
     5701 catcgtagtt atggcaggag cgacaggttg ccgagttgtt ttctttcatt cgcgcccatt
     5761 cacgctcggc aagttccgcg cgtttggctt caaatttttc aggtgtatca atggagtggg
     5821 caataaaggt ctggtagata tcgttgctcg cttccagttt gcgcttcacc atgcctggaa
     5881 tatccggcgg gatatgacag tcatggcatt cagctcgcac gccggaggcg ttctggaaat
     5941 gcaccgactg tttatattct tcatacactg gttgcatact gtggcaactg acacaaaatt
     6001 cggttgtgct ggtgactttg atcccaacgt gtggcaatac aatcagcgca atgccaatca
     6061 caatcccaat agcgaccagc gccagtaccg accaacgagc actgggtcgg cgtagcgcgt
     6121 tccagagttt ccgcataata gcccctgtaa aattatggtt tagtgaagcg atcttaatga
     6181 gtaaatatga acagcggcac tggtcaggat gaacggctta cggcagaata tgaacagata
     6241 tgaacagaat gagtaaaacc ctctgatgcc acatcacatt gttattgttg aagatgagcc
     6301 ggttacccag gcgcgattac aatcctactt cactcaggag gggtataccg tttccgttac
     6361 ggcgagcggt gccgggctac gggaaattat gcagaatcag ccggtggatt taattctgct
     6421 ggatatcaac ttgcccgatg aaaatggcct gatgttaacc cgcgccctgc gagaacgctc
     6481 aacggtgggg attattctgg ttaccggacg cagcgatcgg attgaccgta ttgttgggct
     6541 ggaaatgggc gcagacgatt acgtcaccaa accgctggaa ctgcgcgaac tggtagtacg
     6601 ggtaaaaaat ctgctctggc gaatcgacct cgcgcgacaa gctcaaccgt acactcagga
     6661 caactgctat cgctttgcgg gttattgcct gaatgtgtcg cgccatacgc tggagcggga
     6721 tggtgagccg attaaactga cccgcgcaga gtatgaaatg ttggtggcat ttgtgaccaa
     6781 tccgggcgaa attctcagcc gtgaacgtct gctgcgtatg ctctctgcgc gtcgggtgga
     6841 aaaccctgac ctgcgcaccg tcgatgtgtt aattcgtcgt ttacgccata aactcagcgc
     6901 ggatttactg gtgacgcaac atggcgaagg ttatttctta gccgctgatg tgtgctgata
     6961 aaaatagacc ggacgaaatc ccccaggcga cagcgagcgg cggatatgtt cgcggtcggc
     7021 atttttcggc gtcagaacta aaatcggtgg gctgacatta tcagacaccg attgcccctg
     7081 taattgcctg atggcctgct caactgccag ttccccctgc cagaccattt gatcgctggc
     7141 agccataatc actcttcccc gcttcagccc gcgatacacc tgatgtgaaa gataaaacga
     7201 caccacggta agcggcgttt tcaggttacg cccttccccc attgccgcct ctgccgcaat
     7261 ggccgttccg gcaacgacgt cgatttctgg atggcgttcc agcatctcct gcaacaggtt
     7321 acgctggatt tcaatatcgt tatcaccaag cgcaatatca acaatacgca ccgggcttcc
     7381 ggcaatggct gcgcgaaaac cctcgaccat ctccttactg cccccggcgt tatcgggtcc
     7441 gggcatcaac agcacattca gtggtttacc gtgcgcccat tgcaccagat atcgcccagg
     7501 ttgatagccc atctgaaacc agggcacacc aacgcggctt ttcacctggg gagcatcaat
     7561 agcatttacc agctcgatca ccggcagatt tgctacttgc ttttgcagat cgggaaatga
     7621 cgtcgtgcta ctaccgagca aaatggcctc tgcgccccac tgtttacact ggtcgatttg
     7681 tgcttgctgg gtagccaact ggctgtagcc gcctgcctcc agcactttta aatccacacc
     7741 gtagcggcga gcagcctcct gcataccata gttcaacgat aaccaatatg aatctttcag
     7801 gctgggataa agcgcgcaca gtttccatac gcgtttggct tttagcggca tagaggcttg
     7861 caccgtgaaa tgctgcgcat catgccagcg caacaggtta tcagccgaaa atgccggcaa
     7921 catgaaaagg gaaagaagta aaaatagcag cacgcgcatg atagcctcat caataataag
     7981 gctttatgct agatgcattc cgctttgcga ctcaacattt ttcaccttaa gtgcaccgac
     8041 cgtgaattta accctgaccc gaagactctg gatgggcttt gccctgatgg cgctgttaac
     8101 cctgaccagt accctggtgg gatggtacaa cctgcgcttt atcagccagg tggaaaaaga
     8161 caatactcag gcattgattc ctaccatgaa tatggcgcgc cagttgagcg aagccagcgc
     8221 ctgggaactt ttcgccgcgc agaacctgac cagtgccgat aacgaaaaga tgtggcaggc
     8281 gcaggggcga atgctcaccg cacaaagcct gaagattaat gcgttgctgc aagcgttacg
     8341 ggaacaaggt tttgatacca ccgctattga acaacaggag caggagatct cccgttcgtt
     8401 acgtcagcaa ggggaactgg tggggcggcg tctgcaactg cgccagcaac aacagcgact
     8461 cagtcagcag atagtcgctg ccgccgatga gatcgcacgc ctggcgcaag gtcaggcgaa
     8521 taatgcggca acctccgccg gagcgaccca ggccgggatt tacgatttga tcgaacaaga
     8581 tcagcgtcag gctgctgaaa gtgcactcga tcggctgatt gatatcgatc ttgagtatgt
     8641 taaccagatg aatgaactgc gccttagcgc tctgcgggtg cagcaaatgg tgatgaatct
     8701 ggggctggag cagatccaga aaaatgcgcc aacgctggaa aagcagctca ataatgcggt
     8761 gaaaattctg caacgtcggc aaatacgcat tgaagatccg ggagttcgtg ctcaggtcgc
     8821 aacaacgtta actaccgtta gccaatatag cgatttgctg gcgctgttcc agcaggacag
     8881 tgaaatcagc aatcacctac aaactctcgc acaaaataac atcgcccagt tcgcgcagtt
     8941 tagcagcgaa gtcagtcagc tggtcgacac cattgagctg cgtaatcagc acggactggc
     9001 gcatctggaa aaagccagtg cacgcgggca atacagcctg ttattgctgg ggatggtttc
     9061 actttgcgca ctgattctga tcctctggcg cgtggtttat cgctcagtca cgcgtccact
     9121 tgccgaacaa acgcaggcgc tgcaacggct gctggacggt gatattgact cccctttccc
     9181 ggaaaccgct ggcgtacggg agctggacac catcgggcgg ctgatggatg cgtttcgcag
     9241 cagtgttcat gcactgaatc gccaccgcga acagctggcg gcgcaggtca aagcgcgtac
     9301 agctgaattg caggaactgg tgatagaaca ccgacaagca cgggcggaag cagaaaaagc
     9361 cagccaggca aaatcggcgt ttctggcggc gatgagccat gagatccgca caccgctgta
     9421 cggtattctc ggcaccgcac agctactggc agataacccc gcacttaacg cccagcgtga
     9481 tgatttgcgg gcaattaccg actccggcga atcgttgctg accatcctca acgatattct
     9541 cgattattcc gctatcgaag caggtggcaa gaatgtttcg gtcagcgatg aaccctttga
     9601 accgcgaccg ctgctggaaa gtaccctgca attaatgagt ggacgggtga aaggtcgccc
     9661 gattcgcctg gcaacggcaa ttgccgacga tgtaccgact gcgttaatgg gcgatccgcg
     9721 gcgtattcgt caggtcatta ccaacctgtt gagcaacgcc ctgcgtttta ctgacgaagg
     9781 gcagatcgtt ttacgtagcc gtactgatgg tgagcaatgg ctggtcgaag tggaagacag
     9841 cggctgcggt attgatcccg cgaaactggc agaaatcttc cagccattta ttcaggtaag
     9901 tggcaaacgc ggcggcaccg ggctggggct gactatcagt agccgtctgg cccaggcgat
     9961 gggcggcgaa ctgagcgcca ccagcacgcc ggaggttgga agctgttttt gtttacgctt
    10021 gccgttacgt gttgccacgg cacccgtgcc aaaaacagtc aatcaggcgg tgcgtcttga
    10081 cggtttacgt ttgctgttaa ttgaagataa cccgctaacc cagcgaatta ccgttgagat
    10141 gctgaacacc agtggtgcgc aggttgttgc tattggcaat gccgcgcagg ctttagagac
    10201 actgcaaaat agcgaaccgt ttgctgccgc actggtggat tttgatctgc cggatgtcaa
    10261 cggcattacg cttgcccgac aactggcacg acaatatccg tcgctggttt tgattggctt
    10321 tagcgcccat gtcattgacg aaacgctgcg ccagcgtacc agttcactat ttcgcgggat
    10381 tatccctaaa ccggtgccgc gtgaagtgct cggtcagtta ctggcgcact atctccaact
    10441 gcaagccaat aacgatctac cactggatgt atcgcaactc aatgaagacg ctcagttaat
    10501 ggggacggag aagatccacg aatggctggc attatttaca caacatgccc tgccgcttct
    10561 cgatgaaatc gacattgccc gcgccactca ggatagcgag aaaataaagc gtgccgcgca
    10621 tcagctaaaa agcagttgct cgagtctggg aatgcgtagc gccagccagc tctgcgcaca
    10681 actggagcag cagccattat ctgcccccct tccacacgaa gaaattacac gcagtgttgc
    10741 cgctctggaa gcatggttga ataagaaaga cctgaacgcg atttgaatca atatcagcca
    10801 gttaagaata actagaatag tggcatctgg tatgcaactt aacggactgg caaaatcatg
    10861 gcagagaaaa aaagaacccg ctggcagcgg cggccaggca cgacgggcgg caaattaccg
    10921 tggaatgact ggcacaatgc cacgacctgg cgtaaagcga cgcaattatt gctgttggca
    10981 atgaatattt atattgccat cacgttctgg tattgggtgc gctattacga aacggcaggt
    11041 agtacgatat ttgtcgccag accgggaggt atcgaaggct ggctaccgat tgccggtctg
    11101 atgaatctga aatatagcct tgcaacaggt cagttaccgt ccgtccacgc cgccgcgatg
    11161 ctgttattgg tcgcttttat cgtcatcagt ctattactca aaaaggcctt ttgctcatgg
    11221 ttatgcccgg ttggtacgct ttctgaatta atcggcgctc tcggtaacaa actgtttggt
    11281 cggcaatgtg tccttccccg ctggctggat attcctctgc gcggtgtaaa gtatttgctg
    11341 ttgagttttt ttctctatat cgcgttattg atgcctgctc aggcgattca ctattttatg
    11401 ttgtcgccct acagcgtggt gatggacgtt aaaatgctcg atttctttcg tcatatgggg
    11461 accgcgacat taatcagcgt gaccgttttg ctgattgcca gcctgtttat tcgccatgcc
    11521 tggtgtcgtt atctttgccc atatggcgcg ctgatgggcg tggtttcgct attatcgccg
    11581 tttaagattc gtcgcaatgc cgaaagttgt atcgattgtg gcaaatgcgc aaaaaattgc
    11641 ccatcacgga tcccggtcga taaattaatt caggtacgaa cagtggaatg taccggctgt
    11701 atgacttgcg tagagtcatg tccggtagcc tcaacattga ccttttcact gcaaaaacct
    11761 gcggcaaata aaaaagcctt tgcgttgtct ggctggttaa tgacgctact ggttctgggg
    11821 attatgttta cggtgattgg ttacgcaatg tatgcaggag tatggcaaag cccggtgccg
    11881 gaggaattgt accgacgctt aattccgcaa gcgccaatga ttggtcacta aattagcaac
    11941 aagcggaaca tcacctcgca cggggtgatg ttcacaaaat cacattattt tgattttgac
    12001 atcataactt tcaagcccgg tcattttttc tcgcgcggtg aagcgaattt cagagacttc
    12061 tttccctgta ttcttgttca gctcggcgat tttttgcgcg ataaagtcgg cgatttccgt
    12121 ttcggcttgt tttttaactc ttcaatattc actgttaacc tcttctggtc ttaaaagaca
    12181 gcaacacgcc agtaaggtgt tggttcccgg taccctggtc aggtaagaaa tgttataagc
    12241 catctccccg aaagattcaa taatcaacag gttatcgctt actatacata cagcgttgaa
    12301 aaaaaataag acagatataa aaaaggctcc acaagggagc ctgatattat tttgtgaaat
    12361 ccagcggtgc atggtcagtg cgtatttgca gcgcgacgtt atcaaaggtg atcatcgtcg
    12421 atttacaaaa aatacactta gcgcccaaag ggtttcgctc cgtcacatcg aatgccgatg
    12481 tgcggtactg tgaaccatga caacatggac agcgaaaatg gagatgttga gtaataacgg
    12541 caaccttaga gcgtaactac gttcgctgcc gcggggccac gttgaccctg ctcaatagaa
    12601 aattcaactt tctgattttc gttcagcgtg cggaattcat tgctctggat ggcggtgaaa
    12661 tggacgaaaa cgtctttgct gccatcatca ggagtgataa agccaaaacc tttatctgcg
    12721 ttaaaccatt ttactaaacc agtcatttta ttagacatat tttacttcct tcattttggg
    12781 cctttcggca aacagggctt taatacagaa actacttagt gttcaggaga gagactcaaa
    12841 agaaggggat aatgcctgat aatgagaact gctttagtaa actactttgt atgctgtctg
    12901 tctttccaac cgacgcagct attaacgcat gataattgag attaagcaaa ttttatttta
    12961 gccgtccggc ggtcttaaat atcgctggct ttattttatt aaccccgtat agtgcagcgc
    13021 agtattttca gtaaggaatt tgtttgtccc gtaaaatgac aggaattgtc aaaacctttg
    13081 atcgcaaaag cggcaaagga ttcattatcc cctccgacgg tcgtaaagaa gttcaggtcc
    13141 atatttccgc attcactccc cgcgacgcag aagtgcttat accaggatta cgcgtggaat
    13201 tttgccgcgt aaatggcctg cgaggaccaa cagcggcaaa tgtttatctc tcttaatatg
    13261 acgctgcttg tttaaagctg actggtttta ttggcatagt tcaatagtca acaccatgca
    13321 ttataacaca agttactttt tcaccggaaa actaatccat ttaaccgaat atctctacaa
    13381 ctaatagtta taaatgccac ttaacagcga gttatagtac cacttgaaac tatattgcca
    13441 acaatagtta atagctttct taacaatttt cttatcttta atttgtattt tgctaagatt
    13501 tatcttatgc tcacaggtcg tatttccaga ggggaatagt taacaatctt atatttccct
    13561 ctgcgcgcct ttctcacccg ctgtatatag tgcaagtggt atgaggaaag ggagatttca
    13621 caaggatgtt atactcaaag tagacaatcc tgatctcaag cgtacgtatt gtccatgcga
    13681 tgaataaagg aaaagttatg aaacacaagc tttctgcaat ccttatggcc ttcatgttga
    13741 cgacgccagc tgcgtttgct gcccctgaag cagcaaacgg gaccgaggca acgacgggta
    13801 ctactggcac cacaacgacc actaccggtg caaccacgac ggctgctacc actggtggcg
    13861 tggctgctgg cgctgtcggc acggcgacag ttgtcggtgt ggcaacggct gttggtgtgg
    13921 cgacgttggc agtagttgca gcaaatgaca gtggcgatgg cggttctcat aacacctcaa
    13981 ccaccacgtc cactacgcgc taattctgct taaacgcgtc gaaattaaag ttaaataata
    14041 cccggttgcg atattcgtac cgggctttag agatttgcat ttttttaatt aacctttcaa
    14101 ggattaaagg tgcgccctct tattttatcg attttcgcac tatttcttgc gggatgtacg
    14161 cacagccagc aaagtatggt cgatacattt cgcgccagcc ttttcgataa tcaggatatc
    14221 accgtagcgg atcagcagat ccaggcgttg ccttattcca cgatgtattt acgccttaat
    14281 gaagggcaac gaatctttgt ggtactggga tatatagaac aagaacaaag caaatggtta
    14341 tcccaggata acgccatgct ggttacccac aatggacgtc ttttaaagac cgtcaaactt
    14401 aataataatc tgctggaagt gacaaattcc gggcaggacc ctctgcgtaa cgcgctggca
    14461 ataaaagacg gcagccgctg gacgcgcgat attctctgga gtgaagacaa ccattttcgc
    14521 tctgcgaccc tgagttccac tttttccttt gctggattag agacgctgca tattgcgggt
    14581 cgcgatgtgc tgtgtaatgt ctggcaggaa gaggtgactt ccacgctacc agaaaaacag
    14641 tggcaaaaca cattctgggt cgattcggct actggccagg ttcgtcaaag tcggcaaatg
    14701 ttaggcgcag gggttattcc cgtagaaatg acgtttctta aacccgcacc atgaataaat
    14761 tacagtcgta tttcattgcc agcgtacttt acgtaatgac accccatgcc tttgcgcaag
    14821 gaacggtgac tatttatctg cctggcgaac aacagacact ttccgttggc cccgtggaga
    14881 atgttgccca gttagtgaca caaccgcaac tgcgcgatcg cctctggtgg cctggtgccc
    14941 tgctgaccga ctccgcggtg aaagctaaag cgcttaaaga ctaccaacat gtcatggcac
    15001 agcttgcttc ctgggaagca gaagctgacg acgatgtggc ggcaacgatt aaatccgtgc
    15061 gccagcagtt actcaatctc aatattactg gtcgcttacc ggtcaaactg gaccctgatt
    15121 ttgtacgagt ggatgagaac agcaatcccc cgctggtcgg gaactatacg ctctataccg
    15181 tacagcgccc ggtgactatt actcttctgg gtgcggtttc tggggctggg caattgccct
    15241 ggcaggcagg ccgtagcgtt accgattatc tgcaagatca cccccgtctt gcaggcgcgg
    15301 acaagaataa cgttatcgtg attacgcctg agggtgagac tgtcgtcgcc cctgttgccc
    15361 tgtggaataa aagacatgtg gaaccaccgc ctggcagcca gctttggtta ggcttttctg
    15421 cgcatgtact gcccgaaaaa tatgctgatt taaatgacca gattgtgtct gttttaacgc
    15481 aacgtgttcc tgactgatga agaagaattc ttatctttta agctgcctgg ccattgccgt
    15541 ctccagtgcc tgtcatgctg aagtattaac ctacccggat ccgctgggtt cgtcgcaatc
    15601 ggactttggc ggcacaggat tgttgcagat gccaaatgcg cgcatcgcac cggaaggtga
    15661 attcagcgtc aactaccggg ataacgatca ataccgtttc tactccacct ccgtggcgct
    15721 gttcccatgg ctggaaggca ccattcgtta tacggatgtg cgcacacgta aatatagcca
    15781 gtgggaagat ttcagcggcg atcagtcata caaagacaaa tcattcgatt ttaaacttcg
    15841 cctgtgggaa gaaggttact ggctaccgca agtggcgttt ggtaaacgtg atattgctgg
    15901 tacgggtctg tttgacggtg agtatctggt ggccagcaag caagcggggc catttgattt
    15961 caccctcggg atggcatggg gctacgccgg taatgcgggc aatattacca acccgttttg
    16021 ccgggtgagc gataaatatt gtcatcgcgc agagtctcac gatgcgggcg atatcagctt
    16081 tagcgatatc tttcgtggcc cggcttccat ctttggcggc attgagtatc aaacgccgtg
    16141 gaatcccctg cgtctgaaac tcgaatacga cggcaacaat taccagaatg atttcgctgg
    16201 caaactgcct caggcaagcc atttcaacgt cggtgcagtt tatcgcgctg ccagctgggc
    16261 agacctcaac ctgagttatg aacgcggtaa cacgttgatg tttggtttca ctttacggac
    16321 caatttcaac gatctgcgcc ctgccctgcg cgatacgcca aaaccggcat atcaacctgc
    16381 gcctgaatct gaaggattgc agtacaccac ggtagcaaac caacttaccg ccctgaagta
    16441 taacgcgggc tttgacgcgc cagaaattca gctacgcgat aagacgctgt atatgtctgg
    16501 tcagcaatac aaataccgtg actctcgtga agcggtcgat cgtgccaacc ggattctggt
    16561 gaataacctg ccgcaaggcg ttgagaagat tagcgtgacg caaaagcgcg agcatatggc
    16621 gatggtgact accgaaaccg acgtagccag cctgcgcaaa cagctggcag gtacagcgcc
    16681 tggtcaatca gagccactgc aacaacaacg tgttgaagct gaagatcttt ctgcctttgg
    16741 tcggggctac cgtattcgtg aagatcgctt tagctactct ttcaacccaa cactttcaca
    16801 gtcgctgggc ggcccggaag atttctatat gttccagctg gggctgatgt ccagcgcccg
    16861 ctactggttt accgaccacc tgctgcttga tggcggtatt ttcaccaata tttacaacaa
    16921 ctacgacaag tttaagtctt cgctgttgcc cgcggactct accctgcccc gcgtgcgcac
    16981 gcatatccgt gattacgttc gcaatgacgt ttatctcaac aacttgcaag cgaattactt
    17041 tgccgactta ggcaatggtt tctatggcca ggtgtatggc ggttatctgg aaacgatgta
    17101 tgccggtgtc ggttccgagc tgctttatcg cccgctagat gccagctggg cgctgggtgt
    17161 ggacgttaac tacgtgaagc agcgtgactg ggacaacatg atgcgcttca ccgattattc
    17221 cacgccaact ggtttcgtga cggcttactg gaacccgccg acgctcaatg gcgtactgat
    17281 gaaacttagc gttgggcaat atctggcaaa agataaaggg gcaacgatcg acgtcgccaa
    17341 acgctttgac agcggcgtgg cggtaggggt atgggcggca atcagtaacg tatctaaaga
    17401 tgactacggc gaaggcggct ttagtaaagg tttttatatc tcgattccgt tcgacttgat
    17461 gaccattgga cctaaccgca accgcgcggt ggtttcgtgg acaccattga cgcgtgatgg
    17521 tggacaaatg ctgtcacgca aataccagct ctatccaatg acggcagagc gagaagtacc
    17581 ggttggacaa taacctgacg taattcataa cattacattg actgataaat ataaaccaat
    17641 gaaaaagaac atttttaagt tttcggtact gacattggct gttctgtctt tgacagcatg
    17701 tacccttgtt cctggtcaga atttatcgac cagtaacaag gatgtcattg aactacctga
    17761 caatcagtat gacctggaca aaatggtcaa tatctatccc gtcacgccag gattgattga
    17821 tcagttgcgc gccaaaccga ttatgtcgca ggctaacccg gagctggagc agcaaatcgc
    17881 gaactatgaa tatcgtatcg gcattggtga tgtgttgatg gttacggtct gggatcaccc
    17941 ggaactaacc acccccgcag ggcaataccg tagcgccagt gataccggca actgggttaa
    18001 cgccgatggt gcgattttct acccctatat tggtcgcctg aaagtggccg ggaaaacact
    18061 tacccaggta cgcaacgaga ttacagcgcg tctggattca gtcattgaaa gcccgcaagt
    18121 cgatgtcagc gtcgcggcgt tccgctcgca aaaagcgtac gtcaccggtg aagtcagcaa
    18181 atcgggtcag cagccgatta ccaatattcc tctgaccatc atggatgcga ttaacgccgc
    18241 gggcggtctg accgcagatg cagactggcg caatgtggtg ctcacccaga atggcgtaaa
    18301 gaccaaagtg aacctctatg cgctgatgca gcgtggcgac ttgcgccaga acaaactgct
    18361 gcacccggga gatattttat ttattccgcg caacgatgat ctgaaagtct tcgtgatggg
    18421 tgaagtgggt aaacagagca cgctgaaaat ggaccgcagc ggtatgaccc tggcggaagc
    18481 gctgggtaac gctgagggta tgaatcagga tgtcgctgat gcgacgggga tttttgttat
    18541 tcgtgcaacg caaaacaagc agaatggcaa aatcgccaat atttatcagc tgaatgcgaa
    18601 ggacgcatcg gcaatgatac tcggaacgga attccagctt gaaccttacg acattgttta
    18661 tgtcactacc gcgcctctgg ctcgttggaa ccgtgtgatt tccctgctgg taccgacgat
    18721 ttccggggtg catgacctga cagagacttc acggtggatt caaacatggc ccaactaaaa
    18781 tttaactcaa tcctggtggt ttgtaccggc aacatttgcc gttcgcccat tggcgagcgc
    18841 ttgctgcgta aacgtctgcc gggcgtaaaa gtcaaatcgg caggtgttca tggtctggta
    18901 aaacaccctg cagatgcgac agcggcagat gttgccgcta atcatggcgt atctcttgaa
    18961 ggacatgccg gacgtaagct cactgctgag atggcacgaa attacgatct gattctggca
    19021 atggagtcgg aacatattgc tcaggttacc gcaatcgctc ccgaggtccg cgggaagaca
    19081 atgctgtttg ggcaatggct ggaacagaaa gagatcccgg atccctatcg taaaagtcag
    19141 gatgcatttg aacatgtcta cggaatgttg gagcgcgcca gtcaggaatg ggcgaagcgc
    19201 ctcagccggt aatttgagtt tataaaatac gatgacaact aaaaatatga atacgccacc
    19261 aggcagcact caggaaaatg agatcgatct gcttcgtctg gtcggcgagt tatgggatca
    19321 ccgtaagttt attatcagcg tgaccgcgtt attcacgctg atcgctgtcg cttactcgct
    19381 gttaagcaca ccaatttatc aggcagatac tctggtccag gttgagcaaa aacagggcaa
    19441 cgccattctc agcggcctga gtgatatgat ccctaactca tcgcccgagt ctgcaccgga
    19501 gatccaactg ctgcaatcgc gcatgattct cggtaaaacc attgctgaac tgaatctgcg
    19561 cgacatggtt gagcagaagt attttccgat tgtgggtcgc ggctgggcga gattaaccaa
    19621 agagaaacca ggtgagctgg cgatcagctg gatgcatatt ccacaactga atggtcagga
    19681 tcagcaactg acactcacgg ttggggaaaa cggccactat acgctggaag gtgaagagtt
    19741 caccgtcaat ggtatggtcg gccagcgtct ggaaaaagat ggcgttgcgc tgactatcgc
    19801 ggatattaag gccaaaccag gaacacagtt tgtcctgagc caacgtaccg aactggaagc
    19861 gattaacgcg ttgcaggaaa cctttaccgt tagcgaacgc agtaaagaaa gcgggatgct
    19921 ggaacttacc atgactggtg atgatcccca gttgattact cgtattctga acagcatcgc
    19981 taacaactat ttgcaacaga atatcgctcg ccaggcggcg caggattcac aaagccttga
    20041 attcttacag cgccagttgc cagaagtgcg cagcgagctg gaccaggcgg aagaaaaact
    20101 caacgtttat cgccagcagc gcgattcggt tgaccttaac ctggaagcca aagccgttct
    20161 tgagcagatt gtgaacgttg ataatcaact caatgagctg accttccgcg aggcagagat
    20221 ctcccagctg tataagaaag atcacccaac ttatcgtgcg ctgctggaaa aacgccagac
    20281 gctggagcaa gaacgcaaac gcctgaataa gcgggtatcg gctatgcctt ccacccaaca
    20341 ggaagtgttg cgtttaagtc gtgacgtaga agcgggccgt gcggtatatc tgcaattact
    20401 taaccgccag caggagttga gtatttcgaa atccagtgcc attggtaacg tgcggattat
    20461 cgacccggca gtcactcagc cgcagccagt gaaaccgaaa aaagcgttga atgtggtgct
    20521 tggttttatt cttggcctgt ttatttcggt gggtgccgtg ctggcgcgtg cgatgttgcg
    20581 tcgtggtgta gaagccccgg aacaactgga agagcacggc atcagcgttt atgccaccat
    20641 cccgatgtcc gagtggctgg ataaacgtac ccgtctgcgt aagaaaaatt tattttctaa
    20701 tcagcagcgc catcgtacta aaaatatccc cttcctggcg gtggataacc cggcggattc
    20761 tgctgtggaa gccgtacgtg cgctacgaac cagtctgcat ttcgctatga tggagactga
    20821 gaataacatt ctgatgatca ccggtgcgac gccagacagt ggtaaaacgt ttgtcagttc
    20881 aactctggca gcggtgatcg cccagtccga tcaaaaagtg ttatttattg atgccgactt
    20941 acgccgtggt tattcgcata acctgtttac cgtgagtaat gaacatggct tgtcggaata
    21001 tctggcaggt aaagatgagc tcaacaaagt gatccagcat tttggcaaag gaggctttga
    21061 tgtgattact cgcggtcagg tgccacctaa cccatctgaa ctgctgatgc gcgatcggat
    21121 gcgtcaatta ctggaatggg cgaacgacca ttacgatctg gtgattgttg atacgccgcc
    21181 gatgctggcg gtgagcgatg ctgcggtcgt ggggcgctct gtcggcacca gcctgctggt
    21241 tgcgcgtttt ggcttgaaca ccgccaaaga ggtgagcttg tcaatgcagc gtctggaaca
    21301 ggctggcgtt aatattaaag gcgcaatcct caatggtgtg attaaacgcg ccagcaccgc
    21361 ttacagttac ggctataact attacggtta tagttactcc gagaaagagt aaaactgaag
    21421 gtaaagtgac cggatgcggc gtgaacgcct tttccggtct acgcattaga cagttcttcg
    21481 tttgtcatca gcctcagagc attcaggtag ctgaatgctc ttttttatac attacaaact
    21541 gcacgccggt atgcgtgctt cattcacgat ttgcgtaaaa ccggccaacg aacacatgcc
    21601 ctgcgcattt cgctcttcac atcctgccag ggtcagtttc acctctccgg gcggcgtatt
    21661 taatgacagc ggagttttat cacgcatctg ctgtaaagtc tggaagacca gcgaaacctg
    21721 aatccactgg ctgttatcgc ttagccgacg ccagcgttca aacaccagtt caccacctgg
    21781 cggcgtgtta tccggctgac ctggaagcgt ccagttgagc tccagtgcgc cgccgagatt
    21841 tgccagatta gtatcgtgtc cggcgataaa cagcactgaa gtgggtaatg tcacaccata
    21901 cgcctgtttt tgcggtggat ggggagtcaa cgctatcatg atcaaatcca acaacggggt
    21961 ggcgcggctg cgggcaacct ctggcgtgcg ttgtagtaaa taaaattgcg cgttatgcaa
    22021 acttagcaac gtgttccact ggtgtgaatc agtgatcctt ccccaccccg gctccggcat
    22081 tccctgtgcc tgttgcagga gaaatatttc cgtcagcatt gatgcgaggc ttaccgcacc
    22141 ggttaatgag acattgtcgg cgctcacctt gagttccgat ggtaatgcct gcgttaatga
    22201 acagctttcg tcctgtttct cacggttaag gcacaagttt gattgcggaa aattaagcac
    22261 ccgttccagt tcgcgaaacg ccgtttgccg atgcccggta aagtcagcaa ttgaccctcc
    22321 tgccctgctg aggatcgcgt cagtcacgtt cgcgttatcc agttggcaaa cgccagtttt
    22381 tagaggatta aataacggat cgggactgga cgtatctgcc tgggtatgta cggttattgc
    22441 acagtcaggt gccagcccgg cggcgaaggc ttcgcctgtt ttacgggtac gctcgtcgac
    22501 atcagcaata atcgcgacct gaccaggctg cgggcagccc tttttcgtca gcaatccgtc
    22561 ggctaccaga cgctggcgtt ggtaatgtcc gagataagcg attagctcac caccgcgcgg
    22621 tgtcagccaa cccagtttta ccggccagtt tggccatgcg tctggggtga catcctgcat
    22681 cagttgcgtg gccttggttg gggcacgcac accatgacga ctgacaatca ccacactttc
    22741 cagcttcagc tccggctccg gctcactctg agcgaatgca gattgcgggg ttaacggaat
    22801 cagaagagat aaaaatggga tcaagatcgc tttcatcgat atgtttccgc ttttcatatc
    22861 cgacattatt gattgcctga tacgacgcta atgcgccgca tcagacttac gttcgttaac
    22921 ttttcaatcg cgggtccagc cataccaaca caagggtgga gagcgaacac atcaacaaaa
    22981 tgccgacgaa ccaaagtaaa taccacattg ttcctgctcc ttagtacagc tcgttttcgt
    23041 tacggcggag agtttctgtc gtcatgcgcc cccacatttt gtagtagctc cagagagtgt
    23101 agagcaacac aatgggcaaa aatatcagca cgattaccaa cataatactc agcgtcagct
    23161 gactggaagt gctgtcccac aacgtcaggc tggagattgg actcacgctt gacggcatga
    23221 caaaggggaa cagcgtgatg cctgccgtga aaatcacgcc aaattgcatc aatgacgcca
    23281 tcaaaaatcc ccaacccggg cggccacgat aaatcgccat caccgtcagc aatgggcaga
    23341 agaaccccag cagcgggaag atccacaaaa cgggcgactc gacaaaatta ttcatccagg
    23401 caccaggtag cactgccacc agtttcatta acggattgga aggaccgtta gcatcctggg
    23461 cgagcagtac aaagccatca ataccgaccc acagccagta acccgccagc aaaaagcaca
    23521 gcatcaccaa cagtgcagcg cgtttagtcg acagctgtga acgcagatga atcacaccaa
    23581 cagttttcag ttgtaaccag acgccacctt gcaaaatcac catcccaagg ctgagcaatc
    23641 cgcacaataa agggaatggc gtcagcagtt gccagaagct gccgagatac tccacgcgta
    23701 attgcggagt gaaggcaaac ggcacgccga gcaacaagtt gccgaaggcg ataccgaaga
    23761 ctaccggcgg caccagactg ccgatgacca gaccggcatc ccacatttta cgccagcgtg
    23821 catcggcgat ttttccgcga taatcaaagg ccagcgggcg gaagaacagc gagcacagca
    23881 ccaggatcat cgccacataa aagccggaaa acgccgctgc atacactctg ggccaggcgg
    23941 caaataatgc cccacctgcg aggatcaacc agacctgatt gccttcccag tgcgcaccaa
    24001 cgctattaat cactatccgg cgttcatcat catcacgcgc caccagcggc agcaaacagc
    24061 caatccccat gtcaaatccg tcggagatca taaagaccac caggatcacg ccaatcagca
    24121 gccaccagat gaagcgcaat gtttcataat caaacatgat tttctccttt acccctgttg
    24181 ctgcgtcggt tgttcactct gcatcgcgct cggccccaga cgggcatatt tctgcatcag
    24241 gtagacttcg gcgattaaga acagggtgta aagccctacg atcatgatca gtgagaaagc
    24301 cagttgtcct gtggttaaag cggagtgcgc ggagtatgtc ggtaagatgt cctgtatcgc
    24361 ccacggctga cgaccaaact cggtcataaa ccacccggct tcaatcgcaa tccacggcaa
    24421 cggcaaactc cagagcgcca ttttcagcac ccagcgatgc tggtcgattt tgccacgcag
    24481 cgtctggaca agcgcaatca gcatcaccag tagcagcagg gaaccacagc ccaccatgat
    24541 gcggaaactc cagaataccg gcgcaacctg aggtatcgcg ccacgcatcg ccgcctggta
    24601 ctgtgcggct gtgacatgat tcatatccgg cgcatagcgg gagagcaaca tgccgtagcc
    24661 caggtcgcct tccagctccc ggaatgcctg caacacatgc ggctcacgat tgccttgcga
    24721 tatttcctgc attaacagcc aggccatacg tccgcgttgc aagcgtgggt aggtttcagc
    24781 catcaaattc ttcagacccg gcacgggttt atctaatgag tgagtggcga ggatccctaa
    24841 cagcacggga attttgatgg caaaggcgtt acgctcttga tcctgttccg gccaggcaac
    24901 cacatggaac ggtgcaggag caggttccgt ttgccactcc ccttccatcg ccgccagttt
    24961 taccggttgt acttgcgcga cttcatacgc agaactgtct ccgagttgca gggtaccgat
    25021 aatcgccaga gtaccgaaga cggaaccgat ggcaaacgag cgtaatgcga catcgcgctc
    25081 ccgtccgcgc agtaaatacc aggcgctgat cgccataata aacatggccc cggtcacgta
    25141 gcccgccatt acggtgtgca caaatttcac ctggctgacc gggttaaaga ccagctcgct
    25201 gaagctggtc atctccatac gcagggtgtc gatatcaaaa tgcgcaccgg tcgggtattg
    25261 catccaaccg ttggcattca atatccacaa tgcagagaga tttgaaccga acgccaccag
    25321 ccacgtcacc agcaggtgct ggtatttatt cagccgctgc cagccgaaga agaaaagccc
    25381 gacaaaggtg gattcgagga agaaggccat taatgcttcc atcgccagcg gtgcgccaaa
    25441 aatatcgccc acatagttgg aatagaatga ccagttagta ccaaactgaa actccatggt
    25501 caggccggta gccacgccaa gagcaaagtt gataccgaag agcttacccc agaagcgcgt
    25561 catatcgcgg tagattgttt tgccggtgac cacgtaaatg gtttccatga tagccagcaa
    25621 aaaaatcagc cccagggtaa ggggtacaaa taaaaagtga tacagcgcgg tcagagcaaa
    25681 ctgccagcgc gataaatcaa tgacatccca catgcgcact cctgtaggcg aagagagaaa
    25741 aataacccgt taatggtaga tctccctctt tatcctgaaa ctctacgacc agaggtattc
    25801 gccgattgcg atattcataa gcctgaaagc gatacttacc gtctttttac gtcggtgcag
    25861 cttccgccag ccactgacat acctcactaa gccgctgcgc agagtcgacc aaatcttctg
    25921 gcgctgccag caccacttcc ggtattgggc agatttcata actttccagt aacgggcctt
    25981 ttaaggtgtc cgtacagcgt aaatgccaga catggcgtaa ccccgtggcg ttgatatagc
    26041 tctcgccata gccaatggta cgaatctgaa tatttcccgg cccacagaga cgtgagagaa
    26101 aaagccgatc agcctcgctg atgggtaatt gcgtcagatt aatgctgtgc ggctgcgcgt
    26161 cggggttacg tacatgcgcc agtaactcat gcgccaacgg taggccattc atcaggccat
    26221 caatgggcgg cggtaacagc gaatctgtcg gcaagagatt ttgcgttgcc gcctgccaca
    26281 acgccagcgg cgcgcagcca gcctccagtt tgtcctccag caacttttcg ccgcgacgtc
    26341 tgcgcacccg ccataatccg cagaagatcg cctcctgtat ttcactttcg ctgtcgtcag
    26401 cctgctgaat gcgcactgac acttcacctt ccccgagaag cgtattgata aagtgacgat
    26461 cgtcggcgtt gagatttaac agatcgactg ccagcacctc gcccaacggc ggctggcgtt
    26521 gacttagttg atgttgtaac tcgtttaaca gcgcaatcac ctgcgggctg tgagcacatt
    26581 gctccagggc cgccatactc ggttcatcat tcacctgaca ggtgatcggc agtggattca
    26641 tgctgaaact gtcatcgttc ggttgcgttc ttggccccag cagatggaaa aaagtttcgc
    26701 tcatgaggca cgctcctgct gtggttcgac aagcccacgc atcaggttta tcagttccgc
    26761 ccacgggtga ataccattca gcacgccgcg atagtttccg ccggtaaaca ctaatgtggc
    26821 gggaaagcga aagacgccaa agcgatctcc gatggcttcg ctctgttcaa ggtcagcaat
    26881 cgccacctgc catgtgtagt cgggaaactc gcgcagtaat tcgccaatca ttaccggatt
    26941 atcgctgacc tctggcgtgc gtttcgggtc actgcttaat aacaccacgc cgtctggcgc
    27001 ttgcgtaagc cagtcgtcaa gacggaattc actaaccggc gtccagccgc gcgccagcat
    27061 tcgttgccac aacgcatcaa atggcgtgtc gttgctcatc cctgctcctc cagtgtcatc
    27121 ctgtactggc gcaagcgaac gccttcgtaa ttttccatcg acagacagtc ataattgaga
    27181 cagcgcgatt cattagccgg ttgcggcaca attccccatg cagccagctg cgccagcgcc
    27241 gcctgttccg ctgcgggcag ttgctcccgt gccagttcgc tcaggctgcc gccgtagtcg
    27301 tcgagcattg cgggttgcag accgacgagg gcaatatgtg ctggcagatg tccgcggata
    27361 tctgccagcg ccagcacttc ggagaaactg ttctggtgca ggctcatttt cttcgcgctg
    27421 agataagccg gaatacgttc tccggcatag gttcgcagcg ttcctggttc cagcccgtag
    27481 tcaatggcat cgagaatcaa cagatggctg gcgctttcga cataccccag caagttcagt
    27541 ccctgagtac cgccatcgac aatctccaca gactcgggcc agtggtaatg ggcatacaac
    27601 cgttccgcca cccgcacgcc gaaaccttca tcggcccaca gcaggttgcc cagccccatt
    27661 accaccacgc gttgctcgct catgaacgct ccttgttact tattttgcca aatttgtggc
    27721 tacggtagcc gttgaccatg gtggagatca ccgtgtcgtc ggacatgatg tcttcacgca
    27781 gcgccatata gacatgaccg atcacaaacg cgccaatcag ccacatcccc agccgatgcc
    27841 agctgtgaat gtccattgag ttgcctcccg tccagtagaa aaattccacc acataacgga
    27901 acggcgcaaa aatagcgtac tggctgtgtt cgctgtacag cgcaaagccg gtgatgatca
    27961 taaagaccga catcaggaaa tagccaaaca tcgccgcctg ggcgatgggg ttatggccta
    28021 tatcggcact cggacgtttt gccagaaaca gataccagcg gatttcatac cacacgccct
    28081 gccaccagct tttacgccat actggcacga taaacagctc gcgggagtat cgattgccaa
    28141 caaaagccca gtagatccgc atcagcaaga ccacggtaaa aaccatcccg gcgctgaagt
    28201 gaattaacct gatgtagccc atatagaaca gatacgtcgc ctcgccgctg acggaaggta
    28261 gcggtttgcc gataaagtaa ccggtgacca tcaacaccgc catgcataac accgtcagcc
    28321 agtgccagat acgcactggc gcttcaaaga catagtggct gacaacgttg tcgcttttct
    28381 gttgcatgat gattctcctt cgctgttaac gcacctgcac ggagatcagc tcgctaccgt
    28441 cgtcgcccag cacgtgtgtt gaacaggcga ggcacggatc aaagctgtgc agagtacgca
    28501 ggatctccag cggttgctcg gggatcgcca ttttggtgtt catcagcgcc gcttcataag
    28561 cgccaatctg ccctttggga tcgcgcgggc tggcgttcca ggtggtcggc accacgcact
    28621 ggtagagatc aatcttgcca tcgcgaatgg cggcccagtg gcctaacgcc ccgcgcggcg
    28681 cttcggtaaa accgacacca cggcactctg tcggccaggt tgtgggttcc catttttccg
    28741 tggaggcggt ggcaagattg ccgtttttca ggttagtcat cagcttgttg aagaaatact
    28801 gcaacttacc tgcggcccac tgtgcttcgt gcgcgcggca caaaatgcgg cccaacgttg
    28861 actggatacc ggaaagcggc aggttcagcg ccgacatcat gcgatcgacc gactcaacgg
    28921 tcgcagcatc gcctttgtga taagcgatta acgtgcgtgc cagcggcccc acttccatcg
    28981 cgttaccgcg ccagcgcggc gctttgatcc acgagtagcg ttcctgttca ttcagctgct
    29041 gaatgttggt atcgctgcct ttgacatcgc cggggttgta ccacgggtcg gtgatgccat
    29101 cgaacggatg acgcccgacc tgatcgttgg gataacgata ccaggcgtga tcgacaaact
    29161 cctgcacctg ctgcggatca accaaatcca ctggcagcac attgttgaag tcgccgttaa
    29221 tcaccgcgcc gccaggcatc agcagacttt tctcaccaaa gtcgttggca atatccggga
    29281 atgcgccgta gctgaggaca catttatcag aaagacccgt gccgatttcg ctccacggtt
    29341 tgttgaactg accgatggct aaggcgtcgg ggatcatcac gttgttaatg aagtctgccg
    29401 tgcgggtgat aatcgactgc accaggttca ggcgttccat attgaccgcc ccgaccgcgc
    29461 cgctttcgtc aatgttaatg gcgcaaggca tcccgccgac aatccagttt ggatgcgggt
    29521 ttttaccgcc aaagaccgcg tggattttga caatttcacg ctggaaatcg agagcttcga
    29581 gatagtgggc aaagcccatc aggttggctt ctggcggcaa cttgtactgc gggtgccccc
    29641 agtagccatt gcggaagatc cccaactgcc cgccttcaac gaatttcttc aggcggtttt
    29701 gtacgtcgaa gaaatagcca ggggatgatt tcggccaaga ggagagactt tgcgccagtt
    29761 cggaggtttt ccgcgggtcg gctttcagcg catccaacac atcgatccag tccatcccgg
    29821 caagctgata gaagtgcacc agatgatcgt ggcaccagag cgttgccagc ataatgttgc
    29881 ggatgatatt ggcgttgtcc ggcactttaa taccgatggc atcttcgatg gcgtaaaccg
    29941 aagccagagc gtgtacgcca gtacagacgc cgcagatacg ttcaacgaac gcccacgcat
    30001 cgcgcgggtc gcgcccttgc aggatgatct ccagcccgcg aaacatggtg ccgcaggaga
    30061 cggcattggt gattacattc tgatcgttaa tattcacttc gcagcgcatg tggccttcaa
    30121 tgcgcgtaat cgggtcgacc accaggcggc gtccggcatt attgatggtg tatccctgag
    30181 tttcgtactg agtgctcatg cctgtttatc ctcattgcct ggctgatgtt cggtttctgt
    30241 aggttgctga ttatgacgtc tgcgctggtc aacggcgctg gcgactgcgt gcacaccaac
    30301 agccgctgcc accacaccaa gcgcggttaa accgacggta tcggcggtgg aatgagtacc
    30361 catttgtgga atatcgacca cgcggctgta gaacgaaccg cgatcccaga aaccattttc
    30421 cgcacagccc aggcagccgt gaccagactg gattgggaaa gaaacgccat cattccagcg
    30481 tgtggaggaa caggcgttat aggtggtagg ccctttgcag cccattttgt acaggcaata
    30541 acctttgcgg gcagcgtcat catcccaact ctggacgaac tctccggcgt cgaagtgagc
    30601 gcggcgatag catttatcgt ggatacgctg accataaaac atcagtggac gacccattct
    30661 gtcgacatct ggcaagcgat caaaggtcac catgtaagta atgatggcgc tcatcacatc
    30721 cgggatcggc gggcagccag gtactttgat aatgggtttg tcggtgatga ctttgtcgat
    30781 agacgttgcc tgtgtcggat taggtcgcgc ggcctgcacg cagccccagg acgcacaggt
    30841 tccccaggcg ataatcgcgc tggctccggc agcggcacgt ttgagtttct caataaacgg
    30901 tcgaccgctg ctgatacaga acatcccctg ctcgcccagc ggcggattac cttctactgc
    30961 gaggatatat ttgccattgt attgcgtgat gatgtcttca aagacttctt ccgcctgggt
    31021 tccggcggca gccatcaaag tatcgtcgta atcgagggaa atcagggaaa ggatgacgtc
    31081 ctttgccagc gggtgagcgg agcggataaa agattcggta cagcaggtgc attccagacc
    31141 gtggatccat accaccggaa tgcgcggttt gttctccagc gcccaggcaa tctttggtgc
    31201 cattcccgcg cctaatccca gcgacgtggc agccagacta caatatttga gaaagctgcg
    31261 ccgggtaacg ccctgacgcc gcatggcctg gtaaaatgtt tcctcgttat tcatatcgca
    31321 cgtctcttct ccttgcgaca ccggcaggac accgggcgct tatggtcggc aagcgacgtc
    31381 cctgtagcaa ggcaaaccat cgcttcacgt gagatgctga aaacgaaagc tcatccttct
    31441 gcactggcgc acgtcgccag aaagtattgt taataaagcg tagtgaaact tttgcacaaa
    31501 acaatacaaa ctgtgtggat ttatctttta gcgataaaaa tggacctatt tttctttttg
    31561 ccgggcggag ggatgtttag ccagttgcta aacgagtaaa agagaaggaa tttatggtga
    31621 tgaaatgatc aatctgcttt tatttcacgc tatcaatgca cttttggctg tttttcaggc
    31681 aaacaaacag attaggggtt tacacgccgc atcgggatgt ttatagtgcg cgtcattccg
    31741 gaagtgtggc cgagcggttg aaggcaccgg tcttgaaaac cggcgacccg aaagggttcc
    31801 agagttcgaa tctctgcgct tccgccaaat aatcaagggg ttaccaaatg gtagcccctt
    31861 tgcttttttg gctcttggta taaacttggt ataccaattg gtatattttt attcggatga
    31921 gattatcggt catgagcgga acctcaggtt cctcgtctga tgccgctctt gcaacacgtt
    31981 acgctgccga gtatttttgt aaaacatgga ctgctccagg cctgaaccag gcagaaggat
    32041 acaaagccat ctcagatctg tcgcatcact attttcgtgc agaaggctct agccctcccc
    32101 aatctttttt gtgacaccct gtttcactga acatgaagac actcaccttg tccctgatat
    32161 tcattagcat catcctgatt atttaacttc tctatttagg gaagcccgtc tgatacaaag
    32221 cttctattcc tcgttaaata ttctccattc tggcgccaag ccctatctat agctgattga
    32281 aaacaactct aaaaacgtaa taatatcaaa tagtttattc ctacagccga tcattttgag
    32341 taaaatattg acgagaattt cacaaaaggt agatttcatg taaacgg
//
LOCUS       DAZKIP010000042        32287 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZKIP010000042 DAZKIP010000000
VERSION     DAZKIP010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.042"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            124..423
                     /locus_tag="ACHOUK_002227"
     CDS             124..423
                     /locus_tag="ACHOUK_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9897677.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILVLFILSLTT
                     TLNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGGFPVTNIVLFLILIFFKKR"
     gene            complement(470..1357)
                     /gene="rarD"
                     /locus_tag="ACHOUK_002228"
     CDS             complement(470..1357)
                     /gene="rarD"
                     /locus_tag="ACHOUK_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFP9897678.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1409..1876)
                     /gene="yigI"
                     /locus_tag="ACHOUK_002229"
     CDS             complement(1409..1876)
                     /gene="yigI"
                     /locus_tag="ACHOUK_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFP9897679.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            2041..2910
                     /gene="pldA"
                     /locus_tag="ACHOUK_002230"
     CDS             2041..2910
                     /gene="pldA"
                     /locus_tag="ACHOUK_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFP9897680.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            3043..4872
                     /gene="recQ"
                     /locus_tag="ACHOUK_002231"
     CDS             3043..4872
                     /gene="recQ"
                     /locus_tag="ACHOUK_002231"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFP9897681.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4936..5556
                     /gene="rhtC"
                     /locus_tag="ACHOUK_002232"
     CDS             4936..5556
                     /gene="rhtC"
                     /locus_tag="ACHOUK_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFP9897682.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5618..6238)
                     /gene="rhtB"
                     /locus_tag="ACHOUK_002233"
     CDS             complement(5618..6238)
                     /gene="rhtB"
                     /locus_tag="ACHOUK_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFP9897683.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLDVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6349..7371
                     /gene="pldB"
                     /locus_tag="ACHOUK_002234"
     CDS             6349..7371
                     /gene="pldB"
                     /locus_tag="ACHOUK_002234"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFP9897684.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     IHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7379..8179
                     /gene="yigL"
                     /locus_tag="ACHOUK_002235"
     CDS             7379..8179
                     /gene="yigL"
                     /locus_tag="ACHOUK_002235"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFP9897685.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            8255..9154
                     /gene="bioP"
                     /locus_tag="ACHOUK_002236"
     CDS             8255..9154
                     /gene="bioP"
                     /locus_tag="ACHOUK_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFP9897686.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(9042..9995)
                     /gene="metR"
                     /locus_tag="ACHOUK_002237"
     CDS             complement(9042..9995)
                     /gene="metR"
                     /locus_tag="ACHOUK_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFP9897687.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            10231..12492
                     /gene="metE"
                     /locus_tag="ACHOUK_002238"
     CDS             10231..12492
                     /gene="metE"
                     /locus_tag="ACHOUK_002238"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFP9897688.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPARHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLDEVDEALALGHKVKPV
                     LLGPITWLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKCGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYVQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSMTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12532..13347)
                     /gene="ysgA"
                     /locus_tag="ACHOUK_002239"
     CDS             complement(12532..13347)
                     /gene="ysgA"
                     /locus_tag="ACHOUK_002239"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFP9897689.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGLDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAASAEDGWQRMLEWFKQYGGKKSL"
     gene            13609..14370
                     /gene="udp"
                     /locus_tag="ACHOUK_002240"
     CDS             13609..14370
                     /gene="udp"
                     /locus_tag="ACHOUK_002240"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFP9897690.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            14511..15938
                     /gene="rmuC"
                     /locus_tag="ACHOUK_002241"
     CDS             14511..15938
                     /gene="rmuC"
                     /locus_tag="ACHOUK_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFP9897691.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            16033..16788
                     /gene="ubiE"
                     /locus_tag="ACHOUK_002242"
     CDS             16033..16788
                     /gene="ubiE"
                     /locus_tag="ACHOUK_002242"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFP9897692.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16802..17407
                     /gene="ubiJ"
                     /locus_tag="ACHOUK_002243"
     CDS             16802..17407
                     /gene="ubiJ"
                     /locus_tag="ACHOUK_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFP9897693.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALAGLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALIKRLEKLEAK"
     gene            17404..19044
                     /gene="ubiB"
                     /locus_tag="ACHOUK_002244"
     CDS             17404..19044
                     /gene="ubiB"
                     /locus_tag="ACHOUK_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFP9897694.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            19123..19392
                     /gene="tatA"
                     /locus_tag="ACHOUK_002245"
     CDS             19123..19392
                     /gene="tatA"
                     /locus_tag="ACHOUK_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFP9897695.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            19396..19911
                     /gene="tatB"
                     /locus_tag="ACHOUK_002246"
     CDS             19396..19911
                     /gene="tatB"
                     /locus_tag="ACHOUK_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFP9897696.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19914..20690
                     /gene="tatC"
                     /locus_tag="ACHOUK_002247"
     CDS             19914..20690
                     /gene="tatC"
                     /locus_tag="ACHOUK_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFP9897697.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20732..21514
                     /gene="tatD"
                     /locus_tag="ACHOUK_002248"
     CDS             20732..21514
                     /gene="tatD"
                     /locus_tag="ACHOUK_002248"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFP9897698.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(21511..21999)
                     /gene="rfaH"
                     /locus_tag="ACHOUK_002249"
     CDS             complement(21511..21999)
                     /gene="rfaH"
                     /locus_tag="ACHOUK_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFP9897699.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            22166..23659
                     /gene="ubiD"
                     /locus_tag="ACHOUK_002250"
     CDS             22166..23659
                     /gene="ubiD"
                     /locus_tag="ACHOUK_002250"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFP9897700.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPIS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGEMAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23705..24406
                     /gene="fre"
                     /locus_tag="ACHOUK_002251"
     CDS             23705..24406
                     /gene="fre"
                     /locus_tag="ACHOUK_002251"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFP9897701.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24598..25761)
                     /gene="fadA"
                     /locus_tag="ACHOUK_002252"
     CDS             complement(24598..25761)
                     /gene="fadA"
                     /locus_tag="ACHOUK_002252"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFP9897702.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSVSDIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25771..27960)
                     /gene="fadB"
                     /locus_tag="ACHOUK_002253"
     CDS             complement(25771..27960)
                     /gene="fadB"
                     /locus_tag="ACHOUK_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFP9897703.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVIMKDINDKSLALGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFER
                     VDVVVEAIVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWHYKEDSKGKPKKEEDAAVDDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            28150..29481
                     /gene="pepQ"
                     /locus_tag="ACHOUK_002254"
     CDS             28150..29481
                     /gene="pepQ"
                     /locus_tag="ACHOUK_002254"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFP9897704.1"
                     /translation="MESLASLYKNHIATLQERTRDALTRFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQASEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            29481..30095
                     /gene="yigZ"
                     /locus_tag="ACHOUK_002255"
     CDS             29481..30095
                     /gene="yigZ"
                     /locus_tag="ACHOUK_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFP9897705.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITLLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            30134..31585
                     /gene="trkH"
                     /locus_tag="ACHOUK_002256"
     CDS             30134..31585
                     /gene="trkH"
                     /locus_tag="ACHOUK_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFP9897706.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31597..32142
                     /gene="hemG"
                     /locus_tag="ACHOUK_002257"
     CDS             31597..32142
                     /gene="hemG"
                     /locus_tag="ACHOUK_002257"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFP9897707.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7519 a         8145 c         8808 g         7815 t
ORIGIN      
        1 attgataact atacagtagt aatttagagt gtgtaagttt ttattgatgg ggtatatttt
       61 aattaaatat aattggaatg tatttttatt ctataaacaa tatcttgggt gaatggattc
      121 tatatgggaa atggtaactt ttggcagttg ttcttgggta aagaacctta cagcaaaaaa
      181 tctgattttg ttaaggcatg gttattgtgg tgggctatta ccatccttgt tctatttatt
      241 ttaagcctga ctacgacatt aaatttaaga agtataggta tagggttaag ttttgggttt
      301 tgtcaaatcc ttggtagata tacgatgtct ctaggatgga gtaagtactg gggagttttg
      361 gggggatttc ctgtaacaaa tattgttctt tttcttatct taattttttt caaaaaaaga
      421 taaataagtc agtgggtagg ctctttaaac ggcctaccca tgttaattct taactttttc
      481 tacgctgcgt ataaatcgca tccatcacaa aaattgccag cgccacccaa ataaaggtga
      541 aagtcaccat cttatcggca cccggttttt caccataaaa cgtcacagcc agcaggaaca
      601 tcagcgtcgg gccaatgtac tggaaaaagc ctaacgttga gagacgaagg cgcgtggcag
      661 cggcggtaaa acacaacagc ggtacggtag tgacaatacc ggcggcgatc agcagtaaat
      721 tcagcgacat cgggttttgc cccatatggc tggttgagct gtcggcaata gcaaacaggt
      781 aaatcgccgc cacgggcagc agccacatgg tttcaattaa catgccggtt tgtgcttcta
      841 ccgcaatctt cttgcgcacc aggccgtaga aggcaaaact aaatgccagc cccagcgcga
      901 taataggtag cgaaccaaaa gtccacagct ggactaacac gccacatatc gccagaatca
      961 ccgccagcca ttgcatccgg cggaatcgct cgccgaggaa aatcatcccc agcacaatgt
     1021 tcaccagcgg gttaataaag taaccaaggc tcgcttccag catatggtga ttgttcaccg
     1081 cccagataaa cagtagccag ttgccaccaa tcagcacggc agagactgcc agcataaaaa
     1141 ttttctgtgg cgtctgaatc agcgttttta aataggacca ctggcggcaa atgctcatca
     1201 gcaccaccat aaagaaaaac gaccagatca cgcgatgcgt caggatctca tcggcgggca
     1261 cgtagtaaat caacttgaag tacgctgggg ctatacccca aataaaataa gcggcaagag
     1321 cgagtaatac gccctgccgc gtttgttttg catccatcgg gaatactcat ttttaattgg
     1381 taacagcagt ttacctgctt ttatgtcttc aacctaccat ataggtggcg gtggcactgg
     1441 caatataaag ctgttcttca ttgtgtaatt caacgcgggc gacggcgact ttattgcctg
     1501 cacgcaacag gctactggta gcagtaaaac gctcgcctct gcctgggcgc agataatcga
     1561 cgcgaagatc aatggtcccc atccgcgata gccgctggcg tagttcatct tcactgatgg
     1621 tttcgtggcg ggttaaggta cttcctacgc acaccagacc ggcggcgaca tccagcgccg
     1681 acgcaatgac cccgccgtgc aaaatgcttt gcgcccagtt gcccaccatc attggctgat
     1741 ttttaaaggc cagctgtgcg aactcttttt cgtaacgctc cagttccatc cccaatgcgc
     1801 ggttaaatgg catgtgataa acaaacatct cacccactaa tttcagggct tgttcagcgg
     1861 tcagtacggc agacatatca tccttacact tcattggtta atgaaatgtt gattttatga
     1921 ttctttgttg ttggtttcta ctttaggaag ggataactaa cggcaatgga gttaagtatg
     1981 taaaataccc cgcagaaaaa cattcacctt atcaataatt cgttacggag aacacgaccg
     2041 atgcggactc tgcagggctg gttgttgccg gtgtttatgt tgcctatggc agtatatgca
     2101 caagaggcaa cggtgaaaga ggtgcatgac gcgccagcgg tgcgtggcag tattatcgcc
     2161 aatatgctgc aggagcatga caatccgttc acgctctatc cttatgacac caactacctc
     2221 atctacaccc aaaccagcga tctgaacaaa gaagcgattg ccagttacga ctgggcggaa
     2281 aatgcgcgta aggatgaagt aaagtttcag ttgagcctgg catttccgct gtggcgtggg
     2341 attttaggcc cgaactcggt gttgggtgcg tcttatacgc aaaaatcctg gtggcaactg
     2401 tccaatagcg aagagtcttc accgtttcgt gaaaccaact acgaaccgca attgttcctc
     2461 ggttttgcca ccgattaccg ttttgcaggt tggacgctgc gcgatgtgga gatggggtat
     2521 aaccacgact ctaacgggcg ttcagacccg acctcccgca gctggaaccg cctttatact
     2581 cgcctgatgg cagaaaacgg taactggctg gtagaagtga agccgtggta tgtggtgggt
     2641 aatactgacg ataacccgga tatcaccaaa tatatgggtt actaccagct taaaatcggc
     2701 tatcacctcg gtgatgcggt gctcagtgcg aaaggacagt acaactggaa caccggctac
     2761 ggcggcgcgg agttaggctt aagttacccg atcaccaaac atgtgcgcct ttatactcag
     2821 gtttacagcg gctatggcga atcgctcatc gactataact tcaaccagac ccgtgtcggt
     2881 gtgggggtta tgctaaacga tttgttttga tgaacggttg agtggttggc aaatctggaa
     2941 tccagcatcc aggattaccc tctcagagac taaaagcatt gcagtttctc gcgcaggcgc
     3001 tgaaaatagc gcctgttttt atttcaggca atcggggtga atgtggcgca ggcggaagtg
     3061 ttgaatctgg agtccggagc taaacaggtt ttacaagaaa cctttggcta ccaacagttt
     3121 cgccccggcc aggaagaaat tatcgacact gtgctttccg gtcgcgattg cctggtcgtc
     3181 atgcccaccg gtggcggaaa atccctttgc tatcaaattc ctgccttatt gctaaacggc
     3241 cttaccgtgg ttgtttcacc gctgatttcg ttgatgaaag atcaggtgga tcaactgcaa
     3301 gccaacggcg tggcggcggc gtgccttaac tcgacgcaaa cccgcgaaca gcaacttgaa
     3361 gtgatgacag gctgccgcac cgggcaaatt cgtctgcttt atatcgcccc ggaacgcctg
     3421 atgctggata actttcttga gcatctggcg cactggaatc cggtgttatt agccgttgat
     3481 gaagcgcact gtatctccca atggggccac gatttccgcc cggaatatgc cgcgctcggt
     3541 cagttgcgcc agcggttccc gacgctgccg tttatggcgc tgaccgccac agccgacgac
     3601 accacgcgcc aggatatcgt gcgcctgctg gggctgaacg atccgctgat tcaaatcagc
     3661 agttttgacc gtccgaatat tcgctacatg ctgatggaga aattcaaacc gctcgatcag
     3721 ttgatgcgct acgtgcagga acagcgcggt aagtccggca ttatctactg caacagccgg
     3781 gcgaaagtag aagacaccgc tgcgcgcctg caaagcaagg gtattagcgc ggcggcctat
     3841 catgccgggc tggaaaataa tgtccgcgcc gacgtgcagg agaaattcca gcgcgatgac
     3901 ctgcaaattg tggtggcgac ggtggcgttc ggcatgggca tcaataaacc aaacgttcgc
     3961 ttcgtggtcc actttgatat tccgcgcaat atcgaatcct attatcagga aaccggtcgc
     4021 gccgggcgtg atggcctgcc tgcggaagcg atgttgtttt acgatccagc tgatatggcg
     4081 tggctgcgcc gttgtctgga agagaagccg caggggcagt tgcaggatat cgagcgccac
     4141 aaactcaatg cgatgggcgc gtttgccgaa gcgcaaactt gccgtcgtct ggtattgctg
     4201 aactattttg gcgaagggcg tcaggagccg tgcgggaact gcgatatctg cctcgatccg
     4261 ccgaaacagt acgacggttc aaccgatgct cagattgccc tttccaccat tggtcgtgtg
     4321 aatcagcggt ttgggatggg ttatgtggtg gaagtgattc gtggtgctaa taaccagcgt
     4381 atccgcgact atggtcatga caaactgaaa gtctatggca tgggccgtga taaaagccat
     4441 gaacattggg tgagcgtgat ccgccagctg attcacctcg gcctggtgac gcaaaatatt
     4501 gcccagcatt ctgccctaca actgacagag gccgcgcgcc cggtgctgcg cggcgaatcc
     4561 tctttacaac ttgccgtgcc gcgtatcgtg gcgctcaaac cgaaagcgat gcagaaatcg
     4621 ttcggcggca actatgatcg caaactgttc gccaaattac gcaaactgcg taaatcgatt
     4681 gccgatgaaa gtaatgtccc gccgtacgtg gtgtttaacg acgcaacctt gattgagatg
     4741 gctgaacaga tgccgatcac cgccagcgaa atgctcagcg ttaacggcgt tgggatgcgc
     4801 aagctggaac gctttggtaa accgtttatg gcgctgattc gtgcgcatgt cgatggcgac
     4861 gacgaagagt agtcagcagc ataaaaaagt gccaggatga agactccgta aacgtttccc
     4921 ccgcgagtca aatgtatgtt gatgttattt ctcaccgtcg ccatggtgca cattgtggcg
     4981 cttatgagtc ccggtcccga tttctttttt gtctctcaga ccgctgtcag tcgttcccgt
     5041 aaagaagcga tgatgggcgt gctgggtatt acctgcggcg ttatggtttg ggcagggatt
     5101 gcgttgcttg gcctgcattt gattatcgaa aaaatggcct ggctgcatac gctgattatg
     5161 gttggcggtg gcctgtatct ctgctggatg ggttaccaga tgctgcgcgg cgcgctgaaa
     5221 aaagaggcgg tttctgcacc tgcgccacag gtcgagctgg cgaaaagtgg gcgcagtttc
     5281 ctgaaaggtt tactgaccaa tctcgctaat ccgaaagcga ttatctactt tggctcggtg
     5341 ttctcattgt ttgtcggtga taacgttggc actaccgcgc gctggggcat tttcgcgttg
     5401 attattgtcg aaacgctggc gtggtttact gtggtggcca gcctgtttgc cctgccgcaa
     5461 atgcgccgtg gttatcaacg tctggcgaag tggattgatg gttttgccgg ggcgttattt
     5521 gccggatttg gcattcattt gattatttcg cggtaatgcc agacgcgtct tcagagtaag
     5581 tcggataagg cgtttacgcc gcatccgaca ttatttttca cgcatgcctc gccgatgcta
     5641 acagcgctcc caccagcata aacaacgagc cgaaaatctt attcagcgcc ttcatctgct
     5701 ttggtccttt aatccataga gcaatccgtt gagcaagggt ggcgtaaccg atcatcacaa
     5761 taatatcgac cacaatagtg gtgacatcga gcacgatata ctgcatcagt tgcggctgtt
     5821 gcggcatgat gaattgcgga aatagcgccg ccagaaacac aatacttttg ggattggtga
     5881 gattcacaaa aactgcgcgc tggaacaaat gtcgacgcga ttgagtagag gccagcgatt
     5941 taagatcaat tgcgccagcg gcgcgccatt gctgtattcc cagccaaatc aagtaagccg
     6001 cgcctgccca cttcaacact tcaaacgcaa tcactgagcg ggaaaatagc gtccccaacc
     6061 ccacgccaac cagcacaata tgaatcgcca gtccggtctg aagcccggca atagacgcca
     6121 ccgcgccgcg ataaccgtgg ttgagcgagg tggtcatagt gttgattgca ccagagcctg
     6181 gcgacaggct taaaatgatt gatgtcagta ggtaggcaaa ccaccattct aaggtcatga
     6241 tgaactcccg gtgtgtctat ttttgtgcca caatacgcta ctgtcgcagc gttgtgtcag
     6301 gcacgctaaa aaaaacgatt ttacgtggtt taagaggcag attacccgat gtttcagcag
     6361 caaaaagact gggaaacaag agaaaacgcg tttgctgctt ttaccatggg accgctgact
     6421 gatttctggc gtcagcgtga tgaagcagag tttactggtg tggatgacat tccggtgcgc
     6481 tttgtccgtt ttcgcgcaca gcaccatgac cgggtggtag tcatctgccc ggggcgtatt
     6541 gagagttacg taaaatatgc ggaactggcc tacgatttgt tccatttggg gtttgatgtc
     6601 ttaatcatcg accatcgcgg gcagggacgt tccggtcgcc tgttagccga tccgcatctc
     6661 gggcatgtta atcgctttaa tgattatgtt gatgatctgg cggcattctg gcaacaggag
     6721 gtccagcccg gtccgtggcg taaacgctat atactggcac attcgatggg cggtgcgatc
     6781 tccacattat ttctgcaacg ccatccaggt gtatgtgacg ccattgcgct aactgcgcca
     6841 atgtttggga tcgtgattcg tatgccgtca tttatggcac ggcagatcct caactgggcc
     6901 gaagcgcatc cacgtttccg tgatggctat gcaataggca ccgggcgctg gcgcgcgttg
     6961 ccgtttgcta tcaacgtact gacccacagc agacagcgat atcgacgtaa cttacgcttc
     7021 tatgctgatg acccaacgat tcgcgtcggt gggccgacct accattgggt acgcgaaagt
     7081 attctggctg gcgaacaggt gttagccggt gcgggtgatg acgccacgcc aacgcttctc
     7141 ttgcaggctg aagaggaacg cgtggtggat aaccgcattc atgaccgttt ttgtgaactc
     7201 cgcaccgccg cgggccatcc tgtcgaaggg ggacgaccgc tggtaattaa aggtgcgtac
     7261 catgagatcc tttttgaaaa ggacgcaatg cgctcagttg cgctccacgc catcgttgat
     7321 tttttcaaca ggcataactc acccagcgga aaccgctcta cagaggttta aatttcttat
     7381 gtaccaggtt gttgcgtctg atttagatgg cacgttactt tctcccgacc atacattatc
     7441 cccttacgcc aaagaaaccc tgaagctgct caccgcgcgc ggcatcaact ttgtgtttgc
     7501 gaccggtcgt caccacgttg atgtggggca aattcgcgat aatctggaga ttaagtctta
     7561 catgattacc tccaatggtg cgcgcgttca cgatctggat ggtaatctga tttttgctca
     7621 taacctggat cgcgacattg ccagcgatct gtttggcgta gtcaacgaca atccggacat
     7681 cattactaac gtttatcgcg acgacgaatg gtttatgaat cgccatcgcc cggaagagat
     7741 gcgctttttt aaagaagcgg tgttcaaata tgcgctgtat gagcctggat tactggagcc
     7801 ggaaggcgtc agcaaagtgt tcttcacctg cgattcccat gaacaactgc tgccgctgga
     7861 gcaggcgatt aacgctcgtt ggggcgatcg cgtcaacgtc agtttctcta ccttaacctg
     7921 tctggaagtg atggcgggcg gcgtttcaaa aggccatgcg ctggaagcgg tggcgaagaa
     7981 actgggctac agcctgaagg attgtattgc gtttggtgac gggatgaacg atgccgaaat
     8041 gctgtcgatg gcggggaaag gctgcattat gggcagtgcg caccagcgtc tgaaagacct
     8101 tcatcccgag ctggaagtga ttggtactaa tgccgacgac gcggtgccgc attatctgcg
     8161 taaactctat ttatcgtaat cgttctttat ttggtcagtt gtcaacctga tacttcgcta
     8221 caatggatac tcgttaatca aagagttttc cattgtggcg ctacttatca tcaccacgat
     8281 tctgtgggcc ttctccttta gcttttatgg cgagtacctt gcggggcacg tcgatagcta
     8341 ttttgcggtg ctggtgcgcg ttggcctggc ggcactcgtt tttctgccgt ttctgcgtac
     8401 ccgtggcaat agcctgaaaa cagtcggcct gtatatgctg gtgggcgcga tgcagctcgg
     8461 cgtgatgtat atgctgagtt tccgcgctta tctctacctg acggtttccg aactgctgct
     8521 gttcaccgta ctgacgccgc tctacatcac gctgatttat gacatcatga gtaagcgccg
     8581 tctgcgctgg ggatatgctt ttagtgcctt gttggcggtg attggtgccg ggattattcg
     8641 ctatgatcag gtcaccgacc atttctggac tggcttgttg ctggtgcaac tctccaatat
     8701 cacttttgcc atcggcatgg tgggttacaa acgcctgatg gaaactcgcc cgatgccgca
     8761 gcataacgcc tttgcgtggt tctatcttgg cgcgttcctg gtggcggtaa ttgcatggtt
     8821 cttgctggga aatgcgcaga aaatgccgca aaccacgttg caatggggca ttctggtgtt
     8881 tcttggcgtg gtggcttccg ggattggcta ctttatgtgg aactacggcg caacccaagt
     8941 agacgccgga acgctgggca ttatgaataa tatgcacgtt ccggcagggc tgctggttaa
     9001 cctggctatc tggcaccaac agccgcactg gccaacgttt attacaggcg cgctggtgat
     9061 cctggcctca ctgtgggtgc atcgtaagtg ggtcgctccg cgctcttcac aaacggcaga
     9121 tgatcgcagg cgtgattgcg cgctgagcga ataaacgctt ccgtaactgg ctgacgctgc
     9181 tcgccatcgc gcacggcggc gtacagtcgg ctccacaagc cttcgcccag ggttttggtc
     9241 accaccagac cctggcgctc aaaactctct actacccaat gtggtagcgc ggcaataccc
     9301 atccgcgcgg caaccatctg aatcaacaat aaggtgttat cgacgctttt cagcgacggg
     9361 ctaacgcctg cgggctggag aaaatgccgc cagacatcca gtcggctacg ctgcaccgga
     9421 taaatcaaca gcgtttcgct ggcgagatct tccggcgtga ttcgcgtttt cgccgccagt
     9481 ggatggtcag gtgctaacac cagacgcact tcatagtcga acatcggcga ataatgcagg
     9541 ccactacgcg gcagaatatc ggaggtcatc accagatcca gctcgccctg ctgcaacgca
     9601 ggctgcgggt caaacgttac gcccgattta aaatccatct ccacctgcgg ccagttctta
     9661 tggaaatttt ctaatgcggg cgtcagccac tgaatacagc tatggcactc aatggcaatg
     9721 cgcaggcgcg tctgttgcgg ttcattgcag gcttgcagcg cctggctaat ttgcggcagc
     9781 acctggtttg ccagttgcaa caggatttcc ccctgtggag taaagcgtag cggctggctc
     9841 ttacgcacaa atagccggaa gccaaggcgt tgttccagat cgctaaactg gtgagacagg
     9901 gcggattgcg tttgatgcaa cgtcgccgca gcggctgcga gagagccgca gttccgcaac
     9961 gcttgtagcg ttttcaggtg ttttacttcg atcatgaaag tccttcactt cggcatgaat
    10021 aatttgcgct tgaggaatat acagtaaccg ccaattatgg atgtgtaaac atctggacgg
    10081 ctaaaatcct tcgtctttta aatttatggt gcgttggctg cgtttcttca ccccggtcac
    10141 ttacttcagt aagctcccgg ggatgaataa acttgccgcc ttccctaaat tcaaaatcca
    10201 taggatttac atataattag aggaagaaaa atgacaatat tgaatcacac cctcggtttc
    10261 cctcgcgttg gcctgcgtcg cgagctgaaa aaagcgcaag agagttattg ggcggggaac
    10321 tccacgcgtg aagaactgct ggcggtaggg cgtgaattgc gtgctcgtca ctgggatcaa
    10381 caaaagcaag cgggtatcga cctgctgccg gtgggcgatt ttgcctggta cgatcatgta
    10441 ctgaccacca gtctgctgct gggtaacgtt ccggcgcgtc atcagaacaa agatggttcg
    10501 gtagatatcg acaccctgtt ccgtattggt cgtggacgtg cgccgactgg cgaacctgcg
    10561 gcggcagcgg aaatgaccaa atggtttaac accaactatc actacatggt gccggagttc
    10621 gttaaaggcc aacagttcaa actgacctgg acgcagctgc tggacgaagt ggacgaggcg
    10681 ctggcgctgg gccacaaggt gaaacctgtg ctgctggggc cgattacctg gctgtggctg
    10741 gggaaagtga aaggtgaaca gtttgaccgc ctgagcctgc tgaacgacat tctgccggtt
    10801 tatcagcaag tgctggcaga actggcgaaa tgtggcatcg agtgggtaca gattgatgaa
    10861 cccgcgctgg tactggaact accacaggcg tggctggacg catacaaacc cgcttacgac
    10921 gcgctccagg gacaggtgaa actgctgctg accacctatt ttgaaggcgt aacgccaaat
    10981 ctcgacacga ttactgcgct gcctgttcag gggctgcatg tggaccttgt acatggtaaa
    11041 gatgacgttg ttgaactgca caagcgcctg ccttctgact ggctgctgtc tgcgggtctg
    11101 atcaatggtc gtaacgtctg gcgcgccgat cttactgaga aatatgtgca aattaaggac
    11161 attgtcggca aacgtgattt gtgggtggca tcttcctgct cgttgctgca cagccccatc
    11221 gacctgagcg tggaaacgcg tcttgatgca gaagtgaaaa gctggtttgc cttcgcccta
    11281 caaaaatgcc atgaactggc actgctgcgc gatgcgctga acagtggtga cacggcagct
    11341 ctggcagagt ggagcgcccc aattcaggcg cgtcgtcact ctactcgtgt acataatccg
    11401 gcggtagaaa agcgtctggc ggcgatcacc gcccaggaca gccagcgtgc gaatgtctat
    11461 gaagtgcgtg ctgaagccca gcgtgcgcgt tttaaactgc cagcgtggcc gaccaccacg
    11521 attggttcct tcccgcaaac cacggaaatt cgtaccctgc gtctggattt caaaaagggc
    11581 aatctcgacg ccaataacta ccgcacgggc attgcggaac atatcaggca ggccattgtt
    11641 gaacaggaac gtttgggact ggatgtgctg gtacatggtg aggccgagcg taatgacatg
    11701 gtggaatact ttggcgagca cctcgacggg tttgtcttta cgcaaaacgg ttgggtacag
    11761 agctacggtt cccgctgcgt gaagccaccg attgttattg gtgacgttag tcgcccggca
    11821 ccgattaccg tggagtgggc gaagtatgcg caatctatga ctgataaacc ggtgaaaggg
    11881 atgctgactg gcccggtgac cattctctgc tggtcgttcc cgcgtgaaga tgtcagccgt
    11941 gaaaccatcg ccaaacaaat tgcgctggcg ctgcgtgatg aagtggcgga cctggaagcc
    12001 gcaggaattg gcatcatcca gattgatgaa ccggcgctgc gcgaaggttt accgctgcgt
    12061 cgtagcgact gggatgcata tctccagtgg ggcgtggagg ccttccgtat caacgccgcc
    12121 gtggcgaaag atgacacaca aatccacact cacatgtgtt attgcgagtt caacgacatc
    12181 atggattcga ttgcggcact ggacgcagac gtcatcacca tcgaaacctc gcgttctgac
    12241 atggagttgc tggagtcgtt cgaagagttt gattatccga atgaaatcgg tccaggcgtc
    12301 tatgacattc actcgccaaa cgtaccgagc gtggaatgga ttgaagcctt gctgaagaaa
    12361 gcggcaaaac gcattccggc agagcgcctg tgggtcaacc cggactgtgg cctgaaaacg
    12421 cgcggctggc cagaaacccg cgcggcactg gcgaacatgg tgcaggcggc gcagaacttg
    12481 cgtcgggggt aaaatccaaa ccgggtggta ataccacccg gtcttttctc attacagcga
    12541 cttcttccca ccatactgct taaaccattc cagcatacgc tgccagccat cttctgcaga
    12601 tgcggcatga tagctcgggc gataatcagc gttgaatgca tgcccggcgt ccgggtacac
    12661 gataatctct gctttcgcat tagcagcccg cagcgcctgg cgcatggttt caacgctctc
    12721 ctgcggaata ctgttatcca gaccgccata taagccgaga accggcgcgt taagatcggt
    12781 tgcgatatca acaggttgtt tcggtgaatt cagcgactta tcgcccgtca gtttgccgta
    12841 ccacgccact gcggctttta gctgtggatt atgcgcggca tacagccaag tgatacgtcc
    12901 accccagcag aatccggtga tcattaaacg atgaacatcg ccgccgttgc gggaagccca
    12961 actggcgaca tgatcgagat cggccagcac ctgcgagtca ggcacttttg ctaccagacc
    13021 gctaagcaac gtggggatat cggcaaaatc attcggatcg ccttcgcgga agtaaagttc
    13081 aggtgcgata gccagatacc cctccagcgc cagacggcga caaatgtcgc ggatatgttc
    13141 atgcacgcca aaaatttcct gcactacaat gaccactggc agtgggccat cgctttgctt
    13201 tggtctggca tggtaagcag gcatgttatc cccttgtgaa gggatagagg tgaagcccgc
    13261 cacaatcgcg tcgtccgggg tctgaacgat ggtcgaagcg agaggcgatg cagcaggtgc
    13321 aaatccagat tgttgtgttg ttgccatggt attctccgta cccttataaa aatgttgcgc
    13381 aatgttaact atagtcagca tgcaacaaat cacattgcct gaatcggctc atcttttatg
    13441 cagtcctgca gaatgaagag tgatttatgt gatttgcatc acctttggtg ggtaaattta
    13501 tgcaacgcat ttgcgtcatg gtgatgagta tcacgaaaaa atgttaaacc cttcggtaaa
    13561 gtgtcttttt gcttcttctg actaaaccga ttcacagagg agttgtatat gtccaagtct
    13621 gatgtttttc atctcggcct cactaaaaac gatttacaag gggctacgct tgccatcgtc
    13681 cctggcgacc cggatcgtgt ggaaaagatc gccgcgctga tggataagcc ggttaagctg
    13741 gcatctcacc gcgaattcac tacctggcgt gcagagctgg atggtaaacc tgttatcgtc
    13801 tgctctaccg gtatcggcgg cccgtctacc tctattgctg ttgaagagct ggcacagctg
    13861 ggcattcgca ccttcctgcg tatcggtaca acgggcgcta ttcagccgca tattaatgtg
    13921 ggtgatgtcc tggttaccac ggcgtctgtc cgtctggatg gcgcgagcct gcacttcgca
    13981 ccgctggaat tcccggctgt cgctgatttc gaatgtacga ctgcgctggt tgaagctgcg
    14041 aaatccattg gcgcgacaac tcacgttggc gtgacagctt cttctgatac cttctaccca
    14101 ggtcaggaac gttacgatac ttactctggt cgcgtagttc gtcactttaa aggttctatg
    14161 gaagagtggc aggcgatggg cgtaatgaac tatgaaatgg aatctgcaac cctgctgacc
    14221 atgtgtgcaa gtcagggcct gcgtgccggt atggtagcgg gtgttatcgt taaccgcacc
    14281 cagcaagaga tcccgaatgc tgagacgatg aaacaaactg aaagccaagc ggtgaaaatc
    14341 gtggtggaag cggcgcgtcg tctgctgtaa ttctcttctc ctgtctgaag gccgacgcgt
    14401 tcggcctttt gtatttttgc gtagcgcctc gcaggaaatg cctttccaac tggacgtttg
    14461 tacagcacaa ttctattttg tgcgggtaag ttgttgcgtc aggaggcgtt gtggatttct
    14521 caatcatggt ttacgcagtt attgcgttgg tgggtgtggc aattggctgg ctgtttgcca
    14581 gttatcaaca tgcgcagcaa aaagccgagc aattagctga acgtgaagag atggtcgcgg
    14641 agttaagcgc ggcaaaacaa caaattaccc aaagcgagca ctggcgtgca gagtgcgagt
    14701 tactcaataa cgaagtgcgc agcctgcaaa gtattaacac ctctctggag gccgatctgc
    14761 gtgaagtaac cacgcggatg gaagccgcac agcaacatgc tgacgataaa attcgccaga
    14821 tgattaacag cgagcagcgc ctcagtgagc agtttgaaaa cctcgccaac cgtatttttg
    14881 agcacagcaa tcgccgggtt gatgagcaaa accgtcagag tctgaacagc ctgttgtcgc
    14941 cgctacgtga acaactggac ggtttccgcc gtcaggttca ggacagcttc ggtaaagaag
    15001 cacaagaacg ccataccctg acccacgaaa ttcgcaatct ccagcaactc aacgcgcaaa
    15061 tggcccagga agcgatcaac ctgacgcgcg cgctgaaagg cgacaataaa acccagggca
    15121 actggggcga ggtagtattg acgcgggtgc tggaggcttc cggtctgcgt gaagggtatg
    15181 aatatgaaac ccaggtcagc atcgaaaatg acgcccgctc gcggatgcag ccggatgtca
    15241 tcgtgcggct gccgcaggga aaagatgtgg tgatcgacgc caaaatgacg ctggtcgcct
    15301 atgaacgcta ttttaacgcc gaagacgact acacccgcga aagcgcgcta caggaacata
    15361 tcgcgtcggt gcgtaaccat atccgtttgc tgggacgcaa agattatcaa cagctgccgg
    15421 ggctgcgaac tctggattac gtgctgatgt ttattcccgt tgaacccgct tttttactgg
    15481 cgcttgaccg ccagccggag ctgatcaccg aagcgttgaa aaacaatatc atgctggtta
    15541 gcccgactac gctgctggtg gcgctgcgca ctatcgccaa cctgtggcgt tatgagcatc
    15601 aaagccgcaa cgcccagcaa atcgccgatc gtgccagcaa gctgtacgac aagatgcgtt
    15661 tgttcatcga tgacatgtcc gcgattggtc aaagtctcga caaagcgcag gataactatc
    15721 ggcaggcaat gaaaaaactc tcttcagggc gcggaaatgt gctggcgcag gcagaagcgt
    15781 ttcgcggttt aggagtagaa attaaacgcg agattaatcc ggatttggct gaacaggcgg
    15841 tgagccagga tgaagagtat cgacttcggt cggttccgga gcagccgaat gatgaagctt
    15901 atcaacgcga tgatgaatat aatcagcagt cgcgctagcc cattgggagt agttaagccg
    15961 ggtagaaatc tagggcatcg acgcccaatc tgttacactt ctggaacaat tttttgatga
    16021 gcaggcattg agatggtgga taagtcacaa gaaacgacgc actttggttt tcagaccgtc
    16081 gcgaaggaac aaaaagcgga tatggtcgcc cacgttttcc attctgtggc atcaaaatac
    16141 gatgtcatga atgatttgat gtcatttggt attcatcgtt tgtggaagcg attcacgatt
    16201 gattgcagcg gcgtacgccg tgggcagacc gtgctggatc tggctggtgg caccggcgac
    16261 ctgacagcga aattctcccg cctggtcgga gaaactggca aagtggtcct tgctgatatc
    16321 aatgaatcca tgctcaaaat gggccgcgag aagctgcgta atatcggtgt gattggcaac
    16381 gttgagtatg ttcaggcgaa cgctgaggcg ctgccgttcc cggataacac ctttgattgc
    16441 atcaccattt cgtttggtct gcgtaacgtc accgacaaag ataaagcact gcgttcaatg
    16501 tatcgcgtgc tgaaacccgg cggccgcctg ctggtgcttg agttctcgaa gccaattatc
    16561 gagccgctga gcaaagccta tgatgcatac tccttccatg tgctgccgcg tattggctca
    16621 ctggtcgcga acgacgccga cagctaccgt tatctggcag aatccatccg tatgcatccc
    16681 gatcaggata ccctgaaagc catgatgcag gatgccggat tcgaaagtgt cgactactac
    16741 aatctgacgg caggggttgt ggcgctgcat cgtggttata agttctgaca ggagaccgga
    16801 aatgcctttt aaacctttag tgacggcagg aattgaaagt ctgctcaaca ccttcctgta
    16861 tcgctcaccc gcgctgaaaa cggcccgctc gcgtctgttg ggcaaagtgc tgcgcgtgga
    16921 ggtaaaaggc ttttcgacgt cattgattct ggtgttcagc gaacgccagg ttgatgtact
    16981 gggcgaatgg gcaggcgatg ctgactgcac cgttatcgcc tacgccagtg tgttgccgaa
    17041 acttcgcgat cgccagcagc ttgccgccct gattcgcagt ggtgagctgg aagtgcaggg
    17101 cgatattcag gtggtgcaaa acttcgttgc gctggcaggt ctggcagagt tcgaccctgc
    17161 ggaactgctg gccccttata ccggtgatat cgccgctgaa ggaatcagca aagccatgcg
    17221 cggaggcgca aagttcctgc atcacggcat taagcgccag caacgttatg tggcggaagc
    17281 cattactgaa gagtggcgta tggcacccgg tccgcttgaa gtggcctggt ttgcggaaga
    17341 gacggctgcc gtcgagcgtg ctgttgatgc cctgatcaaa cggctggaaa aactggaggc
    17401 taaatgacgc caggtgaagt acggcgccta tatttcatca ttcgcacttt tttaagctac
    17461 ggacttgatg aactgatccc caaaatgcgt atcaccctgc cgctacggct atggcgatac
    17521 tcattattct ggatgccaaa tcggcataaa gacaaacttt taggtgagcg actacgactg
    17581 gccctgcaag aactggggcc ggtatggatc aagttcgggc aaatgttatc aacccgccgc
    17641 gatctttttc cgccgcatat tgccgatcag ctggcgttat tgcaggacaa agttgctccg
    17701 tttgatggca agctggcgaa gcagcagatt gaagctgcaa tgggcggctt gccggtagaa
    17761 gcgtggtttg acgattttga aatcaagcca ctggcttctg cttctatcgc ccaggttcat
    17821 accgcgcgat tgaaatcgaa tggtaaagag gtggtgatta aagtcatccg cccggatatt
    17881 ttgccggtca ttaaagcaga cctgaaactt atctaccgtc tggctcgctg ggtgccgcgt
    17941 ttgctgccgg atggtcgccg tctacgccca accgaagtgg tgcgcgagta cgaaaagacc
    18001 ttgattgatg aactgaattt gctgcgggaa tctgccaacg ccattcagct tcggcgaaat
    18061 tttgaagaca gcccgatgct ctacatcccg gaggtttacc ctgactattg tagtgaaggg
    18121 atgatggtga tggagcgtat ttacggcatt ccggtgtctg atgttgcggc gctggagaaa
    18181 aacggcacca acatgaaatt gctggcggaa cgcggcgtgc aggtgttctt cactcaggtc
    18241 tttcgcgaca gctttttcca tgccgatatg caccctggca acatcttcgt aagctatgaa
    18301 cacccggaaa acccgaaata tatcggcatt gattgcggga ttgttggctc gctaaacaaa
    18361 gaagataaac gctatctggc agaaaacttt attgccttct ttaatcgcga ctatcgcaaa
    18421 gtggcagagc tacacgtcga ttctggctgg gtgccaccag ataccaacgt tgaagagttc
    18481 gaatttgcta ttcgtacggt ctgtgaacct atctttgaga aaccgctggc cgaaatttcg
    18541 tttggacatg tactgttaaa tctgtttaat acggcgcgtc gcttcaatat ggaagtgcag
    18601 ccgcaactgg tgttactcca gaaaaccctg ctctacgtcg aaggggtagg acgccagctt
    18661 tatccgcaac tcgatttatg gaaaacggcg aagcctttcc tggagtcgtg gattaaagat
    18721 caggtcggta ttcctgcgct ggtgagagca tttaaagaaa aagcgccgtt ctgggtcgaa
    18781 aaaatgccag aactgcctga attggtttac gacagtttgc gccagggcaa gtatttacag
    18841 cacagtgttg ataagattgc ccgcgagctt cagtcaaatc atgtacgtca gggacaatcg
    18901 cgttattttc tcggaattgg cgctacgtta gtattaagtg gcacattctt gttggtcagc
    18961 cgacctgaat gggggctgat gcccggctgg ttaatggcag gtggtctgat cgcctggttt
    19021 gtcggttggc gcaaaacacg ctgatttttt catcgctcaa ggcgggccgt gtaacgtata
    19081 atgcggcttt gtttaatcat catctaccac agaggaacat gtatgggtgg tatcagtatt
    19141 tggcagttat tgattattgc cgtcatcgtt gtactgcttt ttggcaccaa aaagctcggc
    19201 tccatcggtt ccgatcttgg tgcgtcgatc aaaggcttta aaaaagcaat gagcgatgat
    19261 gaaccaaagc aggataaaac cagtcaggat gctgatttta ctgcgaaaac tatcgccgat
    19321 aagcaggcgg atacgaatca ggaacaggct aaaacagaag acgcgaagcg ccacgataaa
    19381 gagcaggtgt aatccgtgtt tgatatcggt tttagcgaac tgctattggt gttcatcatc
    19441 ggcctcgtcg ttctggggcc gcaacgactg cctgtggcgg taaaaacggt agcgggctgg
    19501 attcgcgcgt tgcgttcact ggcgacaacg gtgcagaacg aactgaccca ggagttaaaa
    19561 ctccaggagt ttcaggacag tctgaaaaag gttgaaaagg cgagcctcac taacctgacg
    19621 cccgaactga aagcgtcgat ggatgaacta cgccaggccg cggagtcgat gaagcgttcc
    19681 tacgttgcaa acgatcctga aaaggcgagc gatgaagcgc acaccatcca taacccggtg
    19741 gtgaaagata atgaagctgc gcatgagggt gtaacgcctg ccgctgcaca aacgcaggcc
    19801 agttcgccgg aacagaagcc agaaaccacg ccagagccgg tggtaaaacc tgctgcggac
    19861 gctgaaccga aaaccgctgc accttcccct tcgtcgagtg ataaaccgta aacatgtctg
    19921 tagaagatac tcaaccgctt atcacgcatc tgattgagct gcgtaagcgt ctgctgaact
    19981 gcattatcgc ggtgatcgtg atattcctgt gtctggtcta tttcgccaat gacatctatc
    20041 acctggtatc cgcgccattg atcaagcagt tgccgcaagg ttcaacgatg atcgccaccg
    20101 acgtggcctc gccgttcttt acgccgatca agctgacctt tatggtgtcg ctgattctgt
    20161 cagcgccggt gattctctat caggtgtggg catttatcgc cccagcgctg tataagcatg
    20221 aacgtcgcct ggtggtgccg ctgctggttt ccagctctct gctattttat atcggcatgg
    20281 cgttcgccta ctttgtggtc tttccgctgg catttggctt ccttgccaat accgcgccgg
    20341 aaggggtgca ggtatccacc gacatcgcca gctatttaag cttcgttatg gcgctgttta
    20401 tggcgtttgg tgtctccttt gaagtgccgg tggcaattgt gctgctgtgc tggatgggga
    20461 ttacctcgcc agaagactta cgcaaaaaac gcccgtatgt gctggttggt gcattcgttg
    20521 tcgggatgtt gctgacgccg ccggatgtct tctcgcaaac gctgttggcg atcccgatgt
    20581 actgtctgtt tgaaatcggt gtcttcttct cacgctttta cgttggtaaa gggcgaaatc
    20641 gggaagagga aaacgacgct gaagcagaaa gcgaaaaaac tgaagaataa attcaaccgc
    20701 ccgtcagggc ggttgtcata tggagtacag gatgtttgat atcggcgtta atttgaccag
    20761 ttcgcaattt gcgaaagacc gtgatgatgt tgtagcgcgc gcttttgacg cgggagttaa
    20821 tgggctactc atcaccggca ctaacctgcg tgaaagccag caggcgcaaa agctggcgcg
    20881 tcagtattcg tcctgttggt caacggcggg cgtacatcct cacgacagca gccagtggca
    20941 agctgcgact gaagaagcga ttattgagct ggccgcgcag ccagaagtgg tggcgattgg
    21001 tgaatgtggt ctcgacttta accgcaactt ttcgacgccg gaagagcagg aacgcgcttt
    21061 tgttgcccag ctacgcattg ccgcagattt aaacatgccg gtatttatgc actgtcgcga
    21121 tgcccacgag cggtttatga cattactgga gccgtggctg gataaactgc ctggtgcggt
    21181 tcttcattgc tttaccggca cacgcgaaga gatgcaggcg tgcgtggcgc atggaattta
    21241 tatcggcatt accggttggg tttgcgatga acgacgcgga ctggagctgc gggaactttt
    21301 gccgttgatt ccggcggaaa aattactgat cgaaactgat gcgccgtatc tgctccctcg
    21361 cgatctcacg ccaaagccat catcccggcg caacgagcca gcccatctgc cccatatttt
    21421 gcaacgtatt gcgcactggc gtggagaaga tgccgcatgg ctggctgcca ccacggatgc
    21481 taatgtcaaa acactgtttg ggattgcgtt ttagagtttg cggaactcgg tgttcttcac
    21541 actgtgctta atctctttat taataagatt aagcaatagc atggagcgag cctcaccatc
    21601 gggttcggtg aaaatggcct gaaagccttc gaacgcgcct tcggtaataa tcaccttatc
    21661 acccggataa ggggttgccg gatcgacaat gtctttcggt ttatataccg atagctgatg
    21721 aatcaccgcc gatgggacta tcgctggcga cgcgccaaag cgcacgaagt ggctgacacc
    21781 gcgggtcgcg ttgatagtcg tggtatgaat cacttctgga tcaaattcca caaacaggta
    21841 gttggggaac aatggctcac tgactgcagt acgtttgcca cgcacgattt tttccagggt
    21901 gatcatcggt gccaggcaat tcacagcctg tctttcgagg tgttcctggg cacgttgaag
    21961 ttgcccgcgc ttgcagtaca gtaaatacca ggattgcata atgactctta tccgtttaat
    22021 cggggcgcaa ggatagcaaa agctttacgc taagttaatt atattccccg gtttgcgtta
    22081 taccgtcaga gttcacgcta atttaacaaa tttacagcat cgcaaagatg aacgccgtat
    22141 aatgggcgca gattaagagg ctacaatgga cgccatgaaa tataacgatt tacgcgactt
    22201 cttgacgttg cttgaacagc agggtgagct aaaacgtatc acgctcccgg tggacccgca
    22261 tctggaaatc actgaaattg ctgaccgcac gctgcgtgct ggtgggcctg cgctgttgtt
    22321 tgaaaaccct aaagggtact caatgccggt gctgtgcaac ttgttcggta cgccaaagcg
    22381 cgtagcgatg ggtatgggcc aggaagatgt ttcagcactg cgtgaagtcg gtaaattatt
    22441 agcatttctg aaagaaccag agccgccaaa aggttttcgc gatctgtttg ataagctgcc
    22501 gcagtttaag caggtgttaa acatgccgac aaagcgactg cgcggtgcac cctgccaaca
    22561 aaaaatcgtc tctggcgatg acgtcgatct caaccgtatt cccattatga cctgttggcc
    22621 ggaagatgcc gcgccgctga ttacatgggg gctaaccgtt acacgtggcc ctcataaaga
    22681 gcgacagaat ctgggcattt atcgccagca actgattggt aaaaacaagc tgattatgcg
    22741 ttggctgtcg catcgcggcg gcgcgctgga ttatcaggag tggtgtgcgg cgcatccagg
    22801 tgaacgtttc ccgatctctg tggcgttggg cgctgatccg gcaaccattc tcggtgcagt
    22861 cacaccagta ccagatactt tgtcggaata cgcctttgcc ggattgctac gtggcaccaa
    22921 aaccgaagta gtgaagtgta tttccaatga tctcgaagtg cccgccagtg cggagattgt
    22981 gctggaaggg tatatcgaac aaggcgaaat ggcgccagaa ggaccgtatg gtgaccacac
    23041 tggttactat aacgaagtcg atagtttccc ggtatttacc gtgacgcata ttacccagcg
    23101 tgaagatgcg atttaccatt ccacctatac cgggcgtccg ccagatgaac ccgcggtact
    23161 gggagtggcg ttgaacgaag tatttgttcc cattctgcaa aagcagttcc cggaaattgt
    23221 cgatttttac ctgccgccgg aaggctgctc ttatcgcctg gcggtagtga caatcaaaaa
    23281 acagtacgcc ggacacgcga agcgcgtcat gatgggcgtc tggtcgttct tacgccagtt
    23341 tatgtacact aaatttgtga tcgtttgcga tgatgacgtt aacgcacgcg actggaacga
    23401 tgtgatttgg gcgattacca cccgtatgga cccagcgcgg gatactgttc tggtagaaaa
    23461 tacgcctatt gattatctgg attttgcctc gcctgtctcc gggctgggtt caaaaatggg
    23521 gctggatgcc acgaataaat ggccggggga aacccagcgt gaatggggac gtcccatcaa
    23581 aaaagatcca gatgttgtcg cacatattga cgccatctgg gatgaactgg ctatttttaa
    23641 caacggtaaa agcgcctgat gcgcgtttgt tttgccctat ttatcgatcc gacagagaaa
    23701 gcgcatgaca actttaagct gtaaagtgac ctcggtagaa gctatcacgg ataccgtata
    23761 tcgtgtccgc atcgtgccag acgcggcctt ttcttttcgt gctggtcagt atttgatggt
    23821 agtgatggat gagcgcgaca aacgtccgtt ctcaatggcc tcgacgccgg atgaaaaagg
    23881 gtttatcgag ctgcatattg gcgcttctga aatcaacctt tacgcgaaag cagtcatgga
    23941 ccgcatcctc aaagatcatc aaatcgtggt cgatattccc cacggagaag cgtggctgcg
    24001 cgatgatgaa gagcgtccga tgattttgat tgcgggcggc accgggttct cttatgcccg
    24061 ctcgattttg ctgacagcgt tggcgcgtaa cccaaaccgt gatatcacca tttactgggg
    24121 cgggcgtgaa gagcagcatc tgtatgatct ctgcgagctt gaggcgcttt cgttgaagca
    24181 tcctggtctg caagtggtgc cggtggttga acaaccggaa gcgggctggc gtgggcgtac
    24241 tggcaccgtg ttaacggcgg tattgcagga tcacggtacg ctggcagagc atgatatcta
    24301 tattgccgga cgttttgaga tggcgaaaat tgcccgtgac ctgttttgca gtgagcgtaa
    24361 tgcgcgggaa gatcgcctgt ttggcgatgc gtttgcattt atctgagata taaaaaaacc
    24421 cgcccctgac aggcgggaag aacggcaact aaaactttat tcagtggcat ttagatctat
    24481 gtcgcatccg gcattcggca cacgatgcct gatgcgacgc tggcgcgtct tatcaggcct
    24541 acattggtgc cggatcggta gaccggataa ggcgttcacg ccgcatccgg caagtggtta
    24601 aacccgctca aacaccgtcg caataccctg acccagaccg atacacatcg tcgccagacc
    24661 aaactgaacg tctttgtgtt ccatcagatt cagcagcgtg gtgctgatac gcgcaccgga
    24721 acaacccagc ggatgaccca gcgcgatcgc gccaccgttg aggttgatct tctcgtcaat
    24781 ctgctcaatt agtcccagat ctttaataca tggcaggatc tgcgcggcaa aggcttcatt
    24841 catttcaaac acgccgatat cactgacaga aagccccgct tttttcagcg ccagcttcga
    24901 ggccggaacc gggccgtaac ccatgatcga tgggtcacaa ccaacgaccg ccatcgaacg
    24961 cacacgagcg cgcggcttaa gacctaattc atgggcgcgg ctttcactca tcaccagcat
    25021 ggcagctgcg ccatcggaaa gtgcagaaga tgtgcccgcc gttaccgtac cgtttactgg
    25081 atcaaacgcc ggacgcagcg tggcgagggc ttccacggtg gtttccgggc gaatcacttc
    25141 gtcgtaatta aactgcttca ggacgccgtc ggcatcgtga ccaccagttg ggatgatttc
    25201 atttttaaat gcgcccgact gcgtggcggc ccaggcgcgg gcgtgtgacc gcgcggcaaa
    25261 ggcatcctgc atttcacggc tgataccgtg catacgcgcc agcatttctg ccgttaagcc
    25321 catcatgccc gccgctttgg cgacattgcg gctcaggcca gggtgaaaat cgacgccgtg
    25381 actcatcggc acatggccca tatgctccac gccgccaacc agacatgcct gcgcatcgcc
    25441 ggtcatgatc attcgtgctg cgtcatgcag tgcctgcatg gatgaaccac acaagcgatt
    25501 gacagtaacc gccgggacag agtgcggcac ttctgccagc agcgccgcgt tacgggcgat
    25561 attaaaaccc tgctccagcg tctgctgcac acacccccag taaatatcgt cgagggccgc
    25621 cgcttccagc gccgggttac gcgccagcag gctacgcatt aaatgagcgg agagatcttc
    25681 tgcacgcacg ttacgaaaag caccgccctt cgaacggccc atcggggtgc gaattgcatc
    25741 gacaatgaca acctgttcca ttgtgactcc ttaagccgtt ttcaggtcgc caaccggacg
    25801 ggctggctca accgggggat agtacggttc gttatgacgc gctttattac gcagaccttc
    25861 cggcacttcg tacagcgggc cgaggtgctg atattgctgt gccatatcga ggtattttgc
    25921 gctgccgagg gtgtccagcc agcggaacgc gccgccgtgg aacggaggga agcccaggcc
    25981 gtagaccagc gccatatccg cttctgccgg agtggcgata atgccttctt ccagacagcg
    26041 caccacttcg ttgaccatcg ggatcatcat gcgggcgata atctcttctt cgctgaaatc
    26101 gcgcttcggc tggctcactt ctgccagcag gtcgtcaaca gcggcgtctt cttctttctt
    26161 cggcttacct ttgctgtctt ctttataatg ccagaaaccg aggccgttct tctgaccaaa
    26221 gcggttggca tcaaacagcg cgtcgatggc atcgcgataa tctttctgca tccgctgtgg
    26281 gaagcccgct gccatgactg cctgggcgtg atgcgcggtg tcaatgccca caacgtccag
    26341 cagatatgcc ggacccatag gccagccaaa ctgtttttcc atcactttgt cgatcttgcg
    26401 gaaatccgcg ccgtcgcgca gcaactggct gaaaccggcg aaatacggga acagcacgcg
    26461 gttgacgaag aagccggggc aatcgttaac cacaatcggc gttttaccca tcttgctcgc
    26521 ccaggcgacg actttcgcaa tagtttcgtc ggagcttttc tcgccgcgaa taatttccac
    26581 caacggcatt cggtggaccg ggttaaagaa gtgcatcccg cagaagtttt ccggacgttc
    26641 cagcgcgttg gccagttcgc tgataggaat ggttgaagtg ttagacgcca gcacggtatc
    26701 cgggcgtact ttctgctcgg tttctgccag cacggctttt ttcactttcg ggttttcaac
    26761 aatcgcttct accacaacat ccacgcgctc aaatccggcg tagtcgagcg ttgggtggat
    26821 cgtggagatc acgccagcca gtttcagacc atcgatcttg ccgcgctcaa gctgcttgtt
    26881 cagtagtttc gcggcttcag tcatgccgag ggctaacgac ttgtcgttaa tatctttcat
    26941 aatcaccggc acgcctttcc acgcagactg gtaagcgatg ccgccaccca taatgcctgc
    27001 gcccagcacg gcggcctgtt tcggggtttc aacgtctttg gtgagtttct tcgctttgcc
    27061 ttttacatat tgatcgttaa ggaaaatgcc gaccagtgcg cgagcttcgt tggtatgcgc
    27121 cagcgggaca aagcttttgt tttctaggtt taaggcttct tcacgaccaa aacgagccgc
    27181 agcttcaatg gtttttactg cggtgatggg ggccgggtaa tgttttcctg ccgtttgtgc
    27241 gaccatccct ttagcgatgg tgaagctcat ggtggcttca atcttgctca gttttagcgg
    27301 ttccagcttc ggctgacgtt tcgctttcca gtcgaggtcg ccgttaatgg cctggcgtaa
    27361 aatcgccatc gccccttcaa tcagtttttc tgctttgact acgccatcca ccagaccgat
    27421 tttcagcgcc tgatccgcgc cgacatcttt accggcggca atgatttcca gcgcactgtc
    27481 agcgcccagc atacgtggca tacgtacaga accgccaaag cccggcatga tgcccagttt
    27541 ggtttccggc agaccgatgc gcagatccgg cgtcgccaga cggtaatcgg tcgccagcac
    27601 gcattcgcag ccaccgccca gcgcatagcc attgacggca gcaatggtcg gcaccggcag
    27661 atcttccagg cgattaaaca cgctattggc aaagtgcagc cactgactta actgttcttc
    27721 aggaacgagg aacagggaca aaaattcggt gatatcagca ccgacgataa aggctgcttt
    27781 gttcgaacgc agcagcagcc cttttagatc tgattgctgt tccagcacgc cgatggcctc
    27841 gccgaggctg gcgacggtcg cagtgtcgag tttattaact gaacctgggg catcaaatac
    27901 cagttcggca atgccatctt ccagccagtc aaggtacagg gtgtcgcctt tgtaaagcat
    27961 gtcagtctcc tgaatccgca aggtgatctg gtcgtaccag atgcatcgaa gtgtgtattt
    28021 tgtgttaaaa atatgcaaat aaaagattaa agaaatgccg atctgatcac gctcggcaga
    28081 aatcacgctc tggatgaacg atgtgctaag atgcggagac ttaaggtcaa aaaaacagaa
    28141 gggtaaaaaa tggaatcact ggcctcgctc tataaaaatc atatagctac attgcaggaa
    28201 cgaactcgcg atgcgctgac gcgcttcaag ctggatgcgt tacttattca ctccggcgaa
    28261 ctgttcaacg tttttctcga cgatcatccc tatccgttta aagtgaaccc gcaattcaaa
    28321 gcgtgggtgc cggtaactca ggtgccaaac tgctggctgc tggtggatgg cgtgaataag
    28381 ccgaaactgt ggttctatct gccggttgat tactggcaca acgtcgaacc gctgccgacc
    28441 tccttctgga ctgaagatgt agaagtgatc gcgctgccga aagccgatgg cattggtagt
    28501 ctgttgcctg ctgcgcgcgg caatatcggt tatatcggtc cggtgccgga acgtgcgctg
    28561 caactgggta ttgaggccag caatatcaac ccgaaagggg ttatcgacta cctgcattac
    28621 taccgctcct tcaaaaccga gtacgagctg gcctgtatgc gtgaagcgca gaaaatggcg
    28681 gtcaacggtc atcgcgcggc agaagaagcg ttccgttctg gcatgagcga gttcgatatc
    28741 aatattgcct atctgactgc gaccggtcat cgtgataccg acgtacctta cagcaacatt
    28801 gtggcactca acgaacacgc tgcggtgctg cattacacca aactggatca tcaggcgtcg
    28861 gaagagatgc gcagcttcct gctggatgcc ggggccgaat ataacggcta tgccgctgac
    28921 ctgacccgta cctggtcggc aaaaagtgac aacgattacg cacagctggt gaaagacgta
    28981 aatgatgaac aactggcgct gatcgcgacc atgaaagctg gcgttagcta tgtggattac
    29041 cacatccagt tccatcagcg catcgccaaa ttgctgcgta aacatcaaat catcaccgat
    29101 atgagtgaag aggcgatggt cgaaaacgat cttaccgggc cgtttatgcc gcatggtatc
    29161 ggccatccgc tgggcctgca ggtgcatgac gtcgccggtt ttatgcagga tgatagcggt
    29221 acgcacctcg cggcaccggc aaaatatccg tacctgcgct gcacccgtat tctccagccg
    29281 ggcatggtgt taaccatcga accgggtatc tacttcattg aatcgctgct ggcaccgtgg
    29341 cgtgaagggc agttcagcaa gcacttcaac tggcagaaaa ttgaagcact gaaaccgttc
    29401 ggcggcattc gtatcgaaga caacgtggtg atccacgaaa ataacgtgga aaacatgacc
    29461 cgggatctga aactggcgtg atggaaagct ggttaattcc tgcggcaccg gtcacggtcg
    29521 ttgaagagat caaaaaaagc cgcttcatta cgctgttggc gcataccgat ggcgttgagg
    29581 cggcgaaagc gtttgttgaa tcggtgcggg cagaacaccc cgatgcccgc caccattgcg
    29641 tggcgtgggt ggcgggtgcg ccggatgatt ctcaacagct gggtttctct gacgacgggg
    29701 agccggcggg aacggcaggt aaaccgatgc tcgcccagct aatgggcagc ggcgttgggg
    29761 aaattaccgc tgtggtggtg cgctactacg gcggcatatt gcttggcacc ggtgggttag
    29821 tgaaagcgta tggcggcggc gtgaatcagg cgctgcgcca gctaacgacc caacgcaaga
    29881 cgccattaac cgaatatact ttgcaatgtg aatatagtca gttaaccggc attgaagcgt
    29941 tgctggggca gtgtgacggc aaaattatca acagtgatta tcaggcattc gttctgctgc
    30001 gggtggcgct tccggcggcg aaagtggctg aattttccgc gaagctggcg gattttagcc
    30061 gtggttcatt gcaattgtta gcgattgaag aataatcccc acttcgtttt gcagaactaa
    30121 ggaagcggca gagatgcatt ttcgcgccat tacccgaatc gttggactac tggtcatctt
    30181 attttcaggg accatgatta tccctgggct ggtagcactc atctaccggg atggagcggg
    30241 ccgcgctttt acccagacct tttttgtcgc cctcgccatt ggctctatgc tgtggtggcc
    30301 gaaccgcaaa gagaagggcg aactgaaatc ccgtgagggg tttctgatag tggtgctgtt
    30361 ctggaccgtg ctgggtagtg tcggtgcgct cccttttatc ttctcggaaa gcccgaacct
    30421 cacgattacc gatgcgtttt ttgaatcttt ctctggcctg accaccaccg gggccactac
    30481 gctggtgggg ctggattcgc tccctcatgc catcctcttt tatcgccaga tgctgcaatg
    30541 gtttggcggg atggggatca tcgtgttagc ggttgcgata ctgcctatcc tcggcgtggg
    30601 tgggatgcag ctctatcgcg cagaaatgcc cggcccgctg aaagataaca aaatgcgccc
    30661 gcgaattgcg gaaacggcga aaaccctgtg gttgatttat gtcttgctga ccgtcgcctg
    30721 tgcgctggcg ttgtggtttg ccggaatgga tgcctttgat gccatcggcc atagctttgc
    30781 gactatcgct attggcggct tctcgacaca tgatgccagt atcggttatt tcgatagccc
    30841 gactattaac actatcattg ctatcttcct gctgatctcc ggctgtaact acggtctgca
    30901 cttttcactg ttaagtgggc gtagtctgaa ggtttattgg cgcgatccgg aatttcgcat
    30961 gtttatcggc gtacagttta cgctggtggt tatttgtacc ctcgtactgt ggtttcataa
    31021 tgtctacagt tcggcgctga tgacaattaa ccaggcgttt ttccaggtgg tgtcgatggc
    31081 gacaaccgcc gggtttacga ctgacagcat tgcccgctgg ccgctctttt tgccggtact
    31141 gcttttatgt tcagctttta tcggcggttg tgccgggtca acgggcggtg gcctgaaagt
    31201 gatccgcatc ctgctgctgt ttaagcaggg gaaccgtgaa ctgaaacgac tggtgcatcc
    31261 gaacgccgtc tatagcatta agctggggaa tcgcgcactg ccggaacgta tcctcgaagc
    31321 cgtttgggga tttttctccg cctatgcatt ggtgtttatt gtcagtatgc tggcgattat
    31381 cgccacgggc gtggatgact tttctgcctt tgcctcggtt gttgcgacat tgaataacct
    31441 ggggccaggg cttggcgtgg ttgctgataa ctttaccagt atgaacccgg tggctaaatg
    31501 gatcctgatt gccaacatgc tgtttggtcg tctcgaggtc tttacattgc tggtgctctt
    31561 taccccgact ttctggcgtg aatgatggag taatacgtga aaacattaat tcttttctca
    31621 acaagggacg gacaaacgcg cgagattgcc tcctacttgg cttcggaact gaaagaactg
    31681 gggatccagg cggatgtcgc caatgtgcac cgcattgaag aaccacagtg ggaaaactac
    31741 gaccgtgtgg tcattggtgc ttctattcgc tatggtcact accactctgc gttccaggaa
    31801 tttgtcaaaa agcatgcgac gcggctgaat tcgatgccga gcgcgtttta ctccgtaaac
    31861 ctggtggcgc gtaaaccgga gaagcgcact ccacagacca acagctacgc gcggaagttt
    31921 ctgatgaact cgcaatggcg tcccgatcgc tgcgcggtca ttgccggggc gctgcgttac
    31981 ccacgttatc gctggtacga ccgttttatg atcaagctga ttatgaagat gtcaggcggt
    32041 gaaacggata cgcgcaaaga agttgtctat accgattggg agcaggtggc gaatttcgcc
    32101 cgagaaatcg cccatttaac cgacaaaccg acgctgaaat aagcataaag aataaaaaat
    32161 gcgcggtcag aaaattattt taaatttccc cttgtcaggc cggaataact ccctataatg
    32221 cgccaccact gacacggaac aacggcaaac acgccgccgg gtcagcggga ttctcctgag
    32281 aattccg
//
LOCUS       DAZKIP010000043        32141 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZKIP010000043 DAZKIP010000000
VERSION     DAZKIP010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.043"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(63..1823)
                     /gene="ycaO"
                     /locus_tag="ACHOUK_002258"
     CDS             complement(63..1823)
                     /gene="ycaO"
                     /locus_tag="ACHOUK_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFP9897708.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDERLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            1953..2645
                     /locus_tag="ACHOUK_002259"
     CDS             1953..2645
                     /locus_tag="ACHOUK_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFP9897709.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKSGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            2844..3932
                     /gene="serC"
                     /locus_tag="ACHOUK_002260"
     CDS             2844..3932
                     /gene="serC"
                     /locus_tag="ACHOUK_002260"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFP9897710.1"
                     /translation="MAQIFNFSSGPAMLPVEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NVACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            4003..5286
                     /gene="aroA"
                     /locus_tag="ACHOUK_002261"
     CDS             4003..5286
                     /gene="aroA"
                     /locus_tag="ACHOUK_002261"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFP9897711.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            5455..6219
                     /gene="ycaL"
                     /locus_tag="ACHOUK_002262"
     CDS             5455..6219
                     /gene="ycaL"
                     /locus_tag="ACHOUK_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFP9897712.1"
                     /translation="MKNTKLLLAIVTSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            6392..7075
                     /gene="cmk"
                     /locus_tag="ACHOUK_002263"
     CDS             6392..7075
                     /gene="cmk"
                     /locus_tag="ACHOUK_002263"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFP9897713.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            7186..8859
                     /gene="rpsA"
                     /locus_tag="ACHOUK_002264"
     CDS             7186..8859
                     /gene="rpsA"
                     /locus_tag="ACHOUK_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFP9897714.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            9019..9303
                     /gene="ihfB"
                     /locus_tag="ACHOUK_002265"
     CDS             9019..9303
                     /gene="ihfB"
                     /locus_tag="ACHOUK_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFP9897715.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            9510..11774
                     /locus_tag="ACHOUK_002266"
     CDS             9510..11774
                     /locus_tag="ACHOUK_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFP9897716.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILLAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GQRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVVILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILDQGLWFLADRSLALLFLGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSAHWPLQGRNDKGNNHSCVVK
                     VDDGTNSILLTGDIEAPAEQKMLSRYWQQVQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYTWLDTPHQGQVTVDFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            11811..13559
                     /gene="msbA"
                     /locus_tag="ACHOUK_002267"
     CDS             11811..13559
                     /gene="msbA"
                     /locus_tag="ACHOUK_002267"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFP9897717.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            13556..14542
                     /gene="lpxK"
                     /locus_tag="ACHOUK_002268"
     CDS             13556..14542
                     /gene="lpxK"
                     /locus_tag="ACHOUK_002268"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFP9897718.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     STGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLAQLTSLASGN"
     gene            14579..15811
                     /gene="ycaQ"
                     /locus_tag="ACHOUK_002269"
     CDS             14579..15811
                     /gene="ycaQ"
                     /locus_tag="ACHOUK_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFP9897719.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEYPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     QGIFREQWLADYYRLKRPALAAWREARAEQRQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLGHC
                     PQGLFTDCRTGWEIDPVA"
     gene            15863..16045
                     /gene="ycaR"
                     /locus_tag="ACHOUK_002270"
     CDS             15863..16045
                     /gene="ycaR"
                     /locus_tag="ACHOUK_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFP9897720.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            16042..16788
                     /gene="kdsB"
                     /locus_tag="ACHOUK_002271"
     CDS             16042..16788
                     /gene="kdsB"
                     /locus_tag="ACHOUK_002271"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFP9897721.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKGLETVGDNFLRHLGIYGYRAGFIRRYVTWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAHEVPGTGVDTPEDLERVRAEMR"
     gene            16942..17835
                     /locus_tag="ACHOUK_002272"
     CDS             16942..17835
                     /locus_tag="ACHOUK_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFP9897722.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(17812..18591)
                     /gene="elyC"
                     /locus_tag="ACHOUK_002273"
     CDS             complement(17812..18591)
                     /gene="elyC"
                     /locus_tag="ACHOUK_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFP9897723.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGSSGEPRQE"
     gene            18727..19512
                     /gene="cmoM"
                     /locus_tag="ACHOUK_002274"
     CDS             18727..19512
                     /gene="cmoM"
                     /locus_tag="ACHOUK_002274"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFP9897724.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPTQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            19509..20831
                     /gene="mukF"
                     /locus_tag="ACHOUK_002275"
     CDS             19509..20831
                     /gene="mukF"
                     /locus_tag="ACHOUK_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFP9897725.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALSYANADRLLDMRDEEMALR
                     DEEVTGELPPDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            20812..21516
                     /gene="mukE"
                     /locus_tag="ACHOUK_002276"
     CDS             20812..21516
                     /gene="mukE"
                     /locus_tag="ACHOUK_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFP9897726.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDIDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            21516..25976
                     /gene="mukB"
                     /locus_tag="ACHOUK_002277"
     CDS             21516..25976
                     /gene="mukB"
                     /locus_tag="ACHOUK_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFP9897727.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            26237..28084
                     /gene="ldtD"
                     /locus_tag="ACHOUK_002278"
     CDS             26237..28084
                     /gene="ldtD"
                     /locus_tag="ACHOUK_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFP9897728.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGILPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQQALDKGQLPTFVAGLAPQHPQYA
                     VMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            28265..28813
                     /locus_tag="ACHOUK_002279"
     CDS             28265..28813
                     /locus_tag="ACHOUK_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFP9897729.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            28840..29487
                     /gene="gloC"
                     /locus_tag="ACHOUK_002280"
     CDS             28840..29487
                     /gene="gloC"
                     /locus_tag="ACHOUK_002280"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFP9897730.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDA
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(29538..30728)
                     /gene="aspC"
                     /locus_tag="ACHOUK_002281"
     CDS             complement(29538..30728)
                     /gene="aspC"
                     /locus_tag="ACHOUK_002281"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFP9897731.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRIAADFLAKNTSVKRVWVSNPSWPNHKSVFNSADLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(30913..32001)
                     /gene="ompF"
                     /locus_tag="ACHOUK_002282"
     CDS             complement(30913..32001)
                     /gene="ompF"
                     /locus_tag="ACHOUK_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFP9897732.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQLLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNISGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         7814 a         7833 c         8641 g         7853 t
ORIGIN      
        1 aaaacgcccc gtaatataac tcagacgaat cattaagcct acattacgta ggctatttga
       61 ttttattttg cccagaatgc tgctttggcg cgctgcagct tttcgtaggc cttcaacagc
      121 gactgatgtg cagcaaacgc gtgcagatcg ctatctactg gttgcaagcc ataaaacgcc
      181 gcttcgccgc tcattgccgc actggcggct tccacggcat ctgcgccgta catgcgaaca
      241 aacgcattca gatattgcag cggctggcga tcttcttcct gcgccagtaa taacaacgtt
      301 tgcaggcagc gataatagtt ggcgcgttcc gggctgaata ccgatgagtt aaactccatc
      361 gtccattcgg tccagaccag agcctgttcc agatcgccac cagccagcgc cagcatggct
      421 tttaattcac caatacgcag ggtgtaccaa ccgttatccg acccggtcgc cagacccaac
      481 agctcacgca cgcgggtaaa gtcatcaaaa ccttcttcat ccagttgctc gatgaggttc
      541 aggtaatctt ctttttccca ctcgctgcct ggtagcgaaa gaatcgtttc acgtaaatgg
      601 ctgcccatac tgttattcgc gagccacaga tcttcagccg gataaatatc ggacatgcca
      661 ggcacgataa tacggcaagc ataaacgccc agatgctcgt aatcggcaat ataaacttct
      721 ttatcttctt tgttgaagat agccatcagc gtagcaaact cttcttccgt ggtgccggag
      781 aaattccagt ccacaaacgg ataatcggca tcctgcttga acaggtccca ggagattaaa
      841 ccgctggaat cgataaagtg cgtttcgagg ttggtatgtt cagcgacttc ttcatcatcg
      901 aaggttggcg gagtaaacac atccaaatct ttcaggccac gaccttgcag cagctcggtc
      961 acggtacgtt ccagtgctac gccaaaatca ggatgcgcac cgaaagaggc aaagcaggtg
     1021 ccgttagcag gattaaacag taccacgcaa atcaccggat actggccgcc aagggaacca
     1081 tcatatgcga agatcgggaa accttctgct tccagtgttt cgatcgcttc aaccaccgct
     1141 gggtaacgcg ccagcacgtc tgccgggatt tccggcaagc tgatgctttc agcaataatg
     1201 cggtttttca cgtagcgttc gaaaacttcg gacaacccct gaacgcgtgc ttcgttacgg
     1261 gtattgccag cagacatacc gttagaaacg tacaggttac caatgatatt catcggaata
     1321 taaacggtct gattatcaga ctgacgtgta aacggcagac cgcaaatacc acgatcttcg
     1381 ttaccggatt gtaggtcaat cagcatactg ccggtcaatt cattctccgg atcgtaaaat
     1441 gcgcgcagac gttcatcgag cagcccttct ggcacatcgt cattttcggt cagtgggaac
     1501 catttttcgt tgggataatg cacaaatgga ccgttggcga tggtttcgcc cagccagaaa
     1561 tcagcaaaaa agtagttggt tgagagacgc tcgaaatatt caccgagtgc agaagccagc
     1621 gccgctttct tggttgcgcc tttaccgttg gtaaaacaca gtgcgcactc tttgtcgcga
     1681 atatgtacag accagacgtt aggcacggga ttcagccagg aggcctcttc aatctgaaag
     1741 ccgaggtctg aaagtttttg ctggaagcga gcgatggaat cttccagtgc ggcatctttg
     1801 ccggggataa atgtttgcgt catgaaaatc actttagtcg tacggaaagc gcgcaataat
     1861 acgggtttta tctcaaaggc gctatcaccg ccgccatgcc gatgaactgt tgactatgct
     1921 tttagcggat aaaccacgtt aagagcataa aaatgaaggc gttcgatctc caccgtatgg
     1981 catttgataa agtgcctttt gatttccttg gcgaagttgc actacgtagt ctttatacct
     2041 ttgtactggt ctttttgttc ctcaaaatga ccggaagacg cggtgtgcgg cagatgtcgc
     2101 tgtttgaagt tttaatcatt ctgacgctgg gatcagcggc gggagatgtg gcgttttatg
     2161 atgatgtgcc gatggtcccg gtacttatcg tctttattac tctggcgttg ttataccgcc
     2221 tggtaatgtg gttgatggcg cacagtgaaa aactggaaga tcttctggaa ggcaagccag
     2281 ttgtcattat tgaagatggc gagctggcct ggtcgaaact caataactcc aacatgacgg
     2341 aatttgagtt ctttatggag ctacgattgc gtggcgtgga gcagctgggg caggtacgtc
     2401 tggcgattct cgaaaccaac gggcaaatca gtgtctattt ctttgaagat gacaaggtga
     2461 aatcgggttt acttatttta cccagtgatt gtacgcagcg ttacaaagtg gtgccggagt
     2521 cggcggacta tgcctgtatt cgttgtagtg aaatcattca tatgaaagcg ggggaaaaac
     2581 aattatgtcc gcgctgtgca aatccagaat ggacgaaggc aagtcgggca aaacgggtga
     2641 cctgacagta aaaacatcgg ctttttgcta ataatccgag agattctttt gtgtgatgca
     2701 agccacattt ttgccctcaa cggttttact cattgcgatg tgtgtcactg aatgataaaa
     2761 ccgatagcca caggaataat gtattacctg tggtcgcaat cgattgaccg cgggttaata
     2821 gcaacgcaac gtggtgaggg gaaatggctc aaatcttcaa ttttagttct ggtccggcaa
     2881 tgctaccggt agaggtgctt aaacaggctc aacaggaact gcgcgactgg aacggtcttg
     2941 gtacgtcggt gatggaagtg agtcaccgtg gcaaagagtt cattcaggtt gcagaggaag
     3001 ccgagaagga ttttcgcgat cttcttaatg tcccctccaa ctacaaggta ttattctgcc
     3061 atggcggtgg tcgcggtcag tttgctgcgg taccgctgaa tattctcggt gataaaacca
     3121 ccgcagatta tgttgatgcc ggttactggg cggcaagtgc cattaaagaa gcgaaaaaat
     3181 actgcacgcc taatgtcttt gacgccaaag tgactgttga tggtctgcgc gcggttaagc
     3241 caatgcgtga atggcaactc tctgataatg ctgcttatat gcattactgc ccgaatgaaa
     3301 ccatcgacgg tatcgccatc gacgaaacgc cagacttcgg caaagatgtg gtggtcgccg
     3361 ccgacttctc ttcaaccatt ctttcccgtc cgattgacgt cagccgttat ggtgtaattt
     3421 acgctggcgc gcagaaaaat atcggcccgg caggcctgac aatcgtcatc gtccgtgaag
     3481 atttgctggg caaagcgaat gtcgcgtgtc cgtcgattct ggattattcc atcctcaacg
     3541 ataacggctc catgtttaac acgccgccga catttgcctg gtatctatct ggtctggtct
     3601 ttaaatggct gaaagcgaac ggcggtgtag ctgaaatgga taaaatcaat cagcaaaaag
     3661 cagaactgct atatggggtg attgataaca gcgatttcta ccgcaatgac gtggcgaaag
     3721 ctaaccgttc gcggatgaac gtgccgttcc agttggcgga cagtgcgctt gacaaattgt
     3781 tccttgaaga gtcttttgct gcaggccttc atgcactgaa aggtcaccgt gtggtcggcg
     3841 gaatgcgcgc gtctatttat aatgccatgc cgctggaagg cgttaaagcg ctgacagact
     3901 tcatggttga gttcgaacgc cgtcacggtt aatgccgaaa ttttgcttaa tccccacagc
     3961 cagcctgtgg ggtttttatt tctgttgtag agagttgagt tcatggaatc cctgacgtta
     4021 caacccatcg ctcgtgtcga tggcactatt aatctgcccg gttccaagag cgtttctaac
     4081 cgcgctttat tgctggcggc attagcacac ggcaaaacag tattaaccaa tctgctggat
     4141 agcgatgacg tgcgccatat gctgaatgca ttaacagcgt taggggtaag ctatacgctt
     4201 tcagccgatc gtacacgttg cgaaattatc ggtaacggcg gtccattaca cgcagaaggt
     4261 gccctggagt tgttcctcgg taacgccgga acggcaatgc gtccgctggc ggcagctctt
     4321 tgtctgggta gcaatgatat tgtgctgacc ggtgagccgc gtatgaaaga gcgcccgatt
     4381 ggtcatctgg tggatgcgct gcgcctgggc ggggcgaaga tcacttacct ggaacaagaa
     4441 aattatccgc cgttgcgttt acagggcggc tttactggcg gcaacgttga cgttgatggc
     4501 tccgtttcca gccaattcct caccgcactg ttaatgactg cgcctcttgc gccggaagat
     4561 acggtgattc gtattaaagg cgatctggtt tctaaacctt atatcgacat cacactcaat
     4621 ctgatgaaga cgtttggtgt tgaaattgaa aatcagcact atcaacaatt tgtcgtaaaa
     4681 ggcgggcagt cttatcagtc tccgggtact tatttggtcg aaggcgatgc atcttcggct
     4741 tcttactttc tggcagcagc agcaatcaaa ggcggcactg taaaagtgac cggtattgga
     4801 cgtaacagta tgcagggtga tattcgcttt gctgatgtgc tggaaaaaat gggcgcgacc
     4861 atttgctggg gcgatgatta tatttcctgc acgcgtggtg aactgaacgc tattgatatg
     4921 gatatgaacc atattcctga tgcggcgatg accattgcta cggcggcgtt atttgcaaaa
     4981 ggcaccacca cgctgcgcaa tatctataac tggcgtgtta aagaaaccga tcgcctgttt
     5041 gcgatggcaa cagaactgcg taaagtcggt gcggaagtag aagaggggca cgattacatt
     5101 cgtatcactc caccggaaaa actgaatttt gccgagatcg cgacatacaa tgatcaccgg
     5161 atggcgatgt gtttctcgct ggtggcgttg tcagatacac cagtgacgat tcttgatccc
     5221 aaatgtacgg ccaaaacatt tccggattat ttcgagcagc tggcgcggat tagccaggca
     5281 gcctgaatga ataacgtcca ataaatagcc aaaactttct ttatcaaaac gtcggcacat
     5341 tgccggcgtt ttttttcgga ccttgtgagt cattttgatt aatggtagcg tcgcttgtca
     5401 atgtaagttg ttgatacata atgtttatat atgattaatc aacggatgat tcacatgaag
     5461 aatactaaat tactgctggc gattgtgacc tctgcagcat tactgacggg gtgtcaaaat
     5521 acccacggta ttgataccaa tatggctatc agctccggtt taaatgccta taaagcagca
     5581 acgttaagcg atgccgatgc aaaagcgatt gccaatcagg gctgtgccga aatggacagc
     5641 ggcaatcaag tcgcaagtaa atccagcaag tacggtaaac gtctggcaaa aatcgccaaa
     5701 gcattgggta acaatattaa tggcacgccg gtcaactata aggtttatat gaccagcgac
     5761 gtcaacgcat gggcgatggc gaacggctgt gttcgtgttt acagtggcct gatggacatg
     5821 atgaacgata acgaaattga aggcgttctg ggccatgaac tgggccacgt cgcgttgggt
     5881 cactcgctgg ctgaaatgaa agcttcttat gcgatcgttg ccgcacgcga tgccatttca
     5941 gctaccagcg gtgtggcttc ccagctttcc cgctcacaat tgggtgatat cgcagaaggc
     6001 gctatcaatg cgaaatactc ccgcgataaa gagtccgaag cagatgattt ctcctttgat
     6061 ctgttgaaga aacgtggcat cagcacccag gggctggttg gcagctttga aaaactggct
     6121 agcctggatg gcggtcgcac ccagtccatg tttgactctc acccaccatc aacagagcgt
     6181 gcgcaacaca tccgtgatcg tatcgcctct ggtaagtaaa tcattgtcat ctttcgggct
     6241 ggtcttctgc cagcccgcta taattgcgca ataaatcccc atctgaatac agacaaaact
     6301 ggtttttgca cacaacgtta acgatttgtg gcgtcggcgc gtataatgcg cgcggttatg
     6361 ttaacggtac gcctgtttta aggagataaa gatgacggca attgccccgg ttattaccat
     6421 tgatggccca agcggtgcag ggaaaggcac cttgtgtaag gctatggcgg aagcgttgca
     6481 atggcatctg ctggattcgg gtgcaattta tcgcgtactg gcactagcgg cattacatca
     6541 ccatgttgat gttgcatcgg aagatgcgct ggtgccgctg gcatcccatc tggatgtacg
     6601 ttttgtgtca accaatggca atctggaagt gatcctcgaa ggggaagatg tcagcggcga
     6661 aattcgtact caggaagtgg cgaatgcagc ttcacaagtc gcggcattcc cacgcgttcg
     6721 tgaagcatta ttgcgtcgcc aacgcgcgtt tcgcgaatta ccaggcctga ttgccgatgg
     6781 ccgcgacatg ggaacggtgg tattccctga tgcgccagtg aaaattttcc ttgacgcctc
     6841 ctcggaagaa cgtgcgcatc gccgcatgct acagttgcag gagaagggct ttagtgttaa
     6901 ctttgagcgc cttttggccg agatcaaaga acgcgacgac cgcgatcgta accgagcggt
     6961 agcgccactg gttccggcag ccgatgcttt agtgttggat tccaccacct taagcattga
     7021 gcaagtgatt gaaaaagcgc tacaatacgc gcgccagaaa ttggctctcg cataagcgac
     7081 cgaatttgca gtacccccgt tgcaatggaa tgacagcggg tatgttaaac aaccccatcc
     7141 ggcatggagc caggtggacg ttaaatataa acctgaagat taaacatgac tgaatctttt
     7201 gctcaactct ttgaagagtc cttaaaagaa atcgaaaccc gcccgggttc tatcgttcgt
     7261 ggcgttgttg ttgctatcga caaagacgta gtactggttg acgctggtct gaaatctgag
     7321 tccgccatcc cggctgagca gttcaaaaac gcccagggcg agctggaaat ccaggtaggt
     7381 gacgaagttg acgttgctct ggacgcagta gaagacggct tcggtgaaac tctgctgtcc
     7441 cgtgagaaag ctaaacgtca cgaagcctgg atcacgctgg aaaaagctta cgaagatgct
     7501 gaaactgtta ccggtgttat caacggcaaa gttaagggcg gcttcactgt tgagctgaac
     7561 ggtattcgtg cgttcctgcc aggttctctg gtagacgttc gtccggtgcg tgacactctg
     7621 cacctggaag gcaaagagct tgaattcaaa gtaatcaagc tggatcagaa gcgcaacaac
     7681 gttgttgttt ctcgtcgtgc cgttatcgaa tccgaaaaca gcgcagagcg cgatcagctg
     7741 ctggaaaacc tgcaggaagg catggaagtt aaaggtatcg ttaagaacct cactgactac
     7801 ggtgcattcg ttgatctggg cggcgttgac ggcctgctgc acatcactga catggcctgg
     7861 aaacgcgtta agcatccgag cgaaatcgtc aacgtgggcg acgaaatcac tgttaaagtg
     7921 ctgaagttcg accgcgaacg tacccgtgta tccctgggcc tgaaacagct gggcgaagat
     7981 ccgtgggtag ctatcgctaa acgttatccg gaaggtacca aactgactgg tcgcgtgacc
     8041 aacctgaccg actacggctg cttcgttgaa atcgaagaag gcgttgaagg cctggtacac
     8101 gtttccgaaa tggattggac caacaaaaac atccacccgt ccaaagttgt taacgttggc
     8161 gatgtagtgg aagttatggt tctggatatc gacgaagaac gtcgtcgtat ctccctgggt
     8221 ctgaaacagt gcaaagctaa cccgtggcag cagttcgcgg aaacccacaa caagggcgac
     8281 cgtgttgaag gtaaaatcaa gtctatcact gacttcggta tcttcatcgg cctggacggc
     8341 ggcatcgacg gcctggttca cctgtctgac atctcctgga acgttgcagg cgaagaagca
     8401 gttcgtgaat acaaaaaagg cgacgaaatc gctgcagttg ttctgcaggt tgacgcagaa
     8461 cgtgaacgta tctccctggg cgttaaacag ctcgcagaag atccgttcaa caactgggtt
     8521 gctctgaaca agaaaggcgc tatcgtaacc ggtaaagtaa ctgcagttga cgctaaaggc
     8581 gcaaccgtag aactggctga cggcgttgaa ggttacctgc gtgcttctga agcatcccgt
     8641 gaccgcgttg aagacgctac cctggttctg agcgttggcg acgaagttga agctaaattc
     8701 accggcgttg atcgtaaaaa ccgtgcaatc agcctgtctg ttcgtgcgaa agacgaagct
     8761 gacgagaaag atgcaatcgc aactgttaac aaacaggaag atgcaaactt ctccaacaac
     8821 gcaatggctg aagctttcaa agcagctaaa ggcgagtaat tctctgactc ttcgggattt
     8881 ttattctgaa gtttgttgag tttacttgac agattgcagg tttcgtcctg taatcaagca
     8941 ctaagggcgg ctacggccgc ccttaatcaa tgcagcaaca gcagccgctt aatttgcctt
     9001 taaggaaccg gaggaatcat gaccaagtca gaattgatag aaagacttgc cacccagcaa
     9061 tcgcacattc ccgccaagac ggttgaagat gcagtaaaag agatgctgga gcatatggcc
     9121 tcgactcttg cgcagggcga gcgtattgaa atccgcggtt tcggcagttt ctctttgcac
     9181 taccgcgcac cacgtaccgg acgtaatccg aagactggcg ataaagtaga actggaagga
     9241 aaatacgttc ctcactttaa acctggtaaa gaactgcgcg atcgcgccaa tatttacggt
     9301 taagttttta ctcaaacttg aacgagagaa aagcacctgt cgggtgcttt tttcatttat
     9361 ctaatctgga actggaagct gcctcgcaga gttttgagca gttttcaccc tttcgttaaa
     9421 ttcttctgaa tgtgcctcgg ggaacgcaaa attcccacca gacaaccgct caacaaagtt
     9481 gcacactttc cataaacagg gaggggtgca tgaaaataac gacagtcggc gtatgcataa
     9541 tttgcggaat ttttccgttg ctgattttgc cccaattgcc tgggacatta acccttgcgt
     9601 ttctgactct cttcgcctgc gtactggcat ttatccctgt taaaaccgtc cgttatatcg
     9661 cgctgacgtt gctgtttttc gtttggggca tattatcagc aaagcaaatt ttgttggcag
     9721 gagaaacctt aactggcgcg acgcaggatg caattgttga gatcactgct actgacggca
     9781 tgaccactca ttacggtcaa attacttatc tacaaggtca acgtatattc cctgcgccag
     9841 gccttgtgct gtatggcgaa tatcttccgc aagcggtttg tgccggacaa ctgtggtcaa
     9901 tgaaactcaa agttcgtgcc gttcatggtc aacttaatga tggcggcttt gatagccagc
     9961 gttatgccat tgcccagcat cagccgctca ccggccgctt tctgcaggca agtgttattg
    10021 aaccgaattg tagcctgcgt gcacagtatc tggcgtcact acaaacaacg ctgcaaccct
    10081 atccgtggaa tgcggttatt cttggtttag gtatggggga acggttatcc gtccctaaag
    10141 aaatcaaaaa tatcatgcgt gatactggaa cggcgcattt aatggcgata tcgggattgc
    10201 acatcgcttt tgcggcgttg ctggctgccg gactcattcg cagtggacaa atttttctgc
    10261 ctgggcgctg gatccactgg caaatgccat taattggcgg aatctgctgt gctgcttttt
    10321 atgcctggct gacgggaatg caacctcctg cattgcgtac cgttgtggcg cttgctacgt
    10381 ggggtatgct taagttaagt gggcgacagt ggagtggctg ggatgtatgg atatgttgtc
    10441 tggcggcaat tttgctgatg gatcctgttg tcattctctc gcaaagttta tggctctctg
    10501 ccgctgcggt cgcggcattg atattttggt atcagtggtt tccctgtcct gagtggcaac
    10561 tgccgccggt attgcgtgca gttgtttccc tcatccatct gcaactggga atcacacttc
    10621 tgcttatgcc cgtgcaaatc gtcatttttc atggcattag tctgacctcg tttattgcaa
    10681 atctattagc aattcccttg gtgacattta tcacggttcc gttgatcctc gccgcgatgg
    10741 ttgtgcattt aagcgggccg ttaatcctgg atcaagggtt atggtttctt gccgaccggt
    10801 ctttggcttt acttttcttg gggttaaaga gtttgccaga agggtggatc aacattgctg
    10861 aacgttggca atggctatca ttttccccat ggttcttact ggtggtatgg cgattaaacg
    10921 cctggcgaac gttgccagca atgtgtgtgg ctgtaggctt gctgatgtgc tggccgctgt
    10981 ggcaaaaacc tcgacctgac gagtggcaag tgtacatgct tgatgtcggg caagggctgg
    11041 caatggtgat agccagaaac ggcaaagcga ttctctatga cacgggactg gcctggcctg
    11101 aaggggatag tgggcaacaa ctgattatcc cctggctcca ctggcataat cttgaaccgg
    11161 aaggcgttat cctgagtcat gaacatctgg atcaccgggg agggctggac tcaatattgc
    11221 atacatggcc gatgttatgg atcagaagtc cgttaaactg ggaacatcat cagccctgtg
    11281 tgcgtggcga agcgtggcaa tggcaaggat tgcgtttcag cgcgcactgg cctttacaag
    11341 gtcgcaacga taaaggaaat aaccattcct gtgtggttaa ggttgatgac gggacgaata
    11401 gcattcttct aaccggtgat attgaagccc cagctgaaca aaagatgcta agccgttact
    11461 ggcagcaagt gcaggcaaca ttgcttcagg tacctcacca tggcagtaat acctcatcat
    11521 cattgccatt aattcagcga gtgaatggaa aagtggcact cgcatcggca tcgcgctata
    11581 acgcatggcg actgccctct aacaaagtta agcatcgcta tcaacaacaa ggttatacgt
    11641 ggcttgatac tcctcatcag gggcaagtaa cggtcgattt ttcagcgcaa ggctggcgga
    11701 ttagcagcct cagggagcaa attttacctc gttggtatca tcagtggttt ggcgtgccag
    11761 tggataacgg gtagaatatg cggctatttc aacaaatgct ggttttttga atgcataacg
    11821 acaaagatct ctctacgtgg cagacattcc gccgactgtg gccaaccatt gcgcctttta
    11881 aagcgggtct gatcgtggcg ggcgtagcgt taatcctcaa cgcagccagc gataccttca
    11941 tgttatcgct ccttaagcca cttcttgatg atggctttgg taaaacagat cgctccgtgc
    12001 tggtgtggat gccgctggtg gtgatcgggc tgatgatttt gcgtggtatc accagctatg
    12061 tctccagcta ctgtatctcc tgggtatcag gaaaggtggt aatgaccatg cgtcgccgcc
    12121 tgtttggtca catgatggga atgccggttt cattctttga caaacagtca acgggtacgc
    12181 tgttgtcacg tatcacctac gattccgaac aggttgcttc ttcctcttcc ggcgcactga
    12241 ttactgttgt gcgtgaaggt gcgtcgatca tcggcctgtt catcatgatg ttctattaca
    12301 gttggcaatt gtcgatcatt ttgattgtgc tggcaccgat tgtttcgatt gcgattcgcg
    12361 tagtatcgaa gcgttttcgc aacatcagta aaaacatgca gaacaccatg gggcaggtga
    12421 ccaccagcgc tgaacaaatg ctgaaaggcc ataaagaagt attgattttc ggtggtcagg
    12481 aagtggaaac gaaacgcttc gataaagtca gcaaccgaat gcgtcttcag gggatgaaaa
    12541 tggtttcagc ctcttccatc tctgatccga tcattcagct gatcgcctct ttggcgctgg
    12601 cgtttgttct gtatgcggcg agcttcccaa gtgtcatgga tagcctgact gccggtacga
    12661 ttaccgttgt tttctcttca atgattgcac tgatgcgtcc gctgaaatcg ctgactaacg
    12721 ttaacgccca gttccagcgc ggtatggcgg cttgtcagac actgtttacc attctggaca
    12781 gtgagcagga gaaagacgaa ggtaagcgcg tgatcgagcg tgcgactggc gacgtggaat
    12841 tccgcaacgt cacctttact tatccgggac gtgacgtacc cgcattgcgt aacatcaacc
    12901 tgaaaattcc ggcagggaaa acggtcgctc tggttggacg ctctggttca ggtaaatcaa
    12961 ccatcgccag cctgatcacg cgtttttacg atattgatga aggcgaaatc ctgatggatg
    13021 gtcacgatct gcgcgagtat accctggcgt cgttgcgtaa ccaggttgct ctggtgtcgc
    13081 agaatgtcca tctgtttaac gatacggttg ctaacaacat tgcttacgca cgaactgaac
    13141 agtacagccg tgagcaaatt gaagaagcgg cgcgtatggc ctacgccatg gacttcatca
    13201 ataagatgga taacggtctc gatacagtga ttggtgaaaa cggcgtactg ctctctggcg
    13261 gtcagcgtca gcgtattgct atcgctcgag ccttgttgcg tgatagcccg attctgattc
    13321 tggatgaagc tacctcggct ctggataccg aatccgaacg tgcgattcag gcggcactgg
    13381 atgagttgca gaaaaaccgt acctctctgg tgattgccca ccgcttgtct accattgaaa
    13441 aggcagacga aatcgtggtc gtcgaggatg gtgtcattgt ggaacgcggt acgcataacg
    13501 atttgcttga gcaccgcggc gtttacgcgc aacttcacaa aatgcagttt ggccaatgat
    13561 cgaaaaaatc tggtctggtg aatccccttt gtggcggcta ttgctgccac tctcctggtt
    13621 gtatggcctg gtgagtggcg cgatccgtct ttgctataaa ctaaaactga agcgcgcctg
    13681 gcgtgccccc gtaccggttg tcgtggttgg taatctcacc gcaggcggca acggaaaaac
    13741 cccggtcgtt gtctggctgg tggaacagtt gcaacagcgc ggtattcgcg tgggggtcgt
    13801 atcgcgggga tatggtggta aggctgaatc ttatccgctg ttattgtcgg cagataccac
    13861 cacagcacag gcgggtgatg aacctgtgtt gatttatcaa cgcactgatg cgcctgttgc
    13921 ggtttctccc gttcgttctg atgcggtaaa agccattctg gcgcaacacc ctgatgtgca
    13981 gatcatcgta accgacgacg gtttacagca ttaccgtctg gcgcgtgatg tggaaattgt
    14041 cgttattgat ggtgtgcgtc gctttggcaa tggctggtgg ttgccggcgg ggccaatgcg
    14101 tgagcgagcg gggcgcttaa agtcagttga tgcggtaatc gtcaacggcg gtgtcccccg
    14161 cagcggtgaa atccccatgc atctgctgcc gggtcaggcg gtgaatttac gtaccggtac
    14221 gcgttgtgac gttgctcagc ttgaacatgt ggtggcgatg gcagggattg ggcatccgcc
    14281 gcgctttttt gccacgctga agatgtgcgg cgtacaaccg gaaaaatgtg taccgctggc
    14341 cgatcatcag tctttgaacc atgcggatgt cagcgcgttg gtaagcaccg ggcaaacgct
    14401 ggtaatgact gaaaaagatg cggtgaaatg ccgggccttt gcagaagaaa attggtggta
    14461 tttgcccgtt gacgcacagc tttcaggtga tgaaccagcg aaactgcttg cgcaactaac
    14521 ctcgctggct tctggcaact agttacgccg cggcagcgtt cgattgatgg agtcatgaat
    14581 gtcgctgccg cacctctccc ttgctgatgc gcgtaatctt caccttgccg cgcaaggcct
    14641 gttaaacaaa ccccgccgtc gagcgtcgtt ggaggatatt ccggcaacga tctcccgcat
    14701 gtccttgctg caaatcgata ccatcaatat tgttgcccgt agcccatatc tggtgctttt
    14761 cagtcgtctg ggaaattatc ctgcccagtg gctggatgag tctctggcgc gtggcgaatt
    14821 aatggaatac tgggcgcatg aagcctgctt tatgccacgc agtgactttc gtcttattcg
    14881 ccaccgcatg ctggcacctg aaaaaatggg ctggaaatac aaagacgcct ggatgcagga
    14941 acatgaggcg gaaattgcac agttaattca gcatattcat gataaggggc cggtacgttc
    15001 agccgatttt gagtatcctc gtaaaggtgc aagcggctgg tgggaatgga agccgcataa
    15061 acggcatctg gaaggtttat ttactgccgg aaaggtgatg gtgattgaac ggcgcaactt
    15121 ccagcgcgtt tatgatttaa cccaccgtgt catgcctgac tgggatgatg agcgcgatct
    15181 cgtttcgcaa acagaagcag aaatcatcat gctggataac agtgcgcgta gccagggaat
    15241 attccgcgaa cagtggctgg cagattacta tcggctgaaa cgtccggcac tggcagcgtg
    15301 gcgcgaagcg agggctgaac agcggcaaat cattgctgtg catgttgaaa aattgggcaa
    15361 tctttggctg catgctgatt tgctgccgct actcgagcga gcgctggccg gaaagctcac
    15421 tgcaacgcac agcgcggtac tttcgccttt tgatcctgtt gtctgggatc gcaaacgcgc
    15481 agagcagctt tttgatttta gctaccggct ggagtgctat accccagcgc cgaaacgcca
    15541 gtatggctat tttgttctgc cgttattaca tcgtgggcaa ttagttgggc gaatggatgc
    15601 caaaatgcat cgccagacag gcatccttga agttatctct ctgtggttac aggaaggtat
    15661 taaaccaacg acaacgctgc aaaaagggtt acgtcaggcg attactgatt tcgctaactg
    15721 gcagcaggca acgcgggtga cattaggaca ctgcccgcaa ggtctcttta cggattgccg
    15781 caccggctgg gaaatagacc ccgtcgcata aatgattatg ataagattta tgcattcata
    15841 acccgatcca tctggaggaa ctatggatca tcgtctgctt gaaatcattg cctgcccggt
    15901 ttgcaacgga aaactttggt ataaccagga aaaacaagaa cttatttgca aactggacaa
    15961 cctggctttc ccgctgcgcg atggcattcc agtgttgctg gaaacggaag cccgcgtgct
    16021 gactgctgat gagagtaaat catgagtttt gtggtcatta ttcccgcgcg ctacgcgtcc
    16081 acgcgtctgc ccggtaaacc attggttgat attaacggca aacccatgat tgttcatgtt
    16141 cttgaacgcg cgcgcgaatc aggtgccgag cgcatcatcg tggcaaccga tcatgaggat
    16201 gtcgcccgcg ccgttgaagc cgctggcggt gaagtatgta tgacgcgcgc cgatcaccag
    16261 tcagggacag aacgcttggc ggaagttgtc gaaaaatgcg cgttcagcga cgacacggtg
    16321 atcgttaatg tgcagggtga tgaaccgatg atccctgcga caatcattcg tcaggttgct
    16381 gataacctcg ctcagcgtca ggtgggtatg gcgactctgg cggtgccaat ccacaatgcg
    16441 gaagaagcgt ttaacccgaa tgcggtgaaa gtggttctcg acgctgaagg gtatgcactg
    16501 tacttctcac gcgctaccat tccgtgggat cgtgatcgtt ttgcaaaagg ccttgaaacc
    16561 gttggcgata acttcctgcg tcatcttggt atttatggct accgtgcagg ctttatccgt
    16621 cgttacgtca cctggcagcc aagcccgtta gaacacatcg aaatgttaga gcagcttcgt
    16681 gttttgtggt acggcgaaaa aatccatgtt gctgtcgcgc atgaagttcc tggcacaggt
    16741 gtggataccc ctgaagatct cgagcgcgtt cgcgctgaaa tgcgctaatt tcacttcacg
    16801 acacttcagc caattttggg aggagtgtcg taccgttacg attttcctca atttttcttt
    16861 tcaacaattg atctcattca ggtgacatct tttatattgg cgctcattat gaaagcagta
    16921 gcttttatga gggtaatctg aatggaacag ctgcgtgccg aattaagcca tttactgggc
    16981 gaaaaactca gtcgtattga gtgcgtcaat gaaaaagcgg atacggcgtt gtgggctttg
    17041 tatgacagcc agggaaaccc aatgccgcta atggcaagaa gctttagtac gcccggaaaa
    17101 gcccgacaac tggcatggaa aaccaccatg ctggcaagaa gtgggactgt ccgtatgccg
    17161 actatttatg gtgtgatgac gcatgaagaa caccccggcc ctgatgtcct gctactggag
    17221 cggatgcgtg gtgtttcggt agaggcacca gcccgaacac cagaacgttg ggaacagctc
    17281 aaagaccaaa tcgttgaagc cttactggcc tggcaccgtc aggacagtcg cggttgcgtc
    17341 ggcgcggtcg acaatactca ggaaaatttc tggccctcat ggtaccggca acatgttgaa
    17401 gtgctatgga ccacgctcaa tcagttcaat aacaccggtc tgacgatgca ggataagcgg
    17461 atcctgtttc gcactcgcga atgtctcccg gcattatttg aaggttttaa cgacaattgt
    17521 gtgctgattc acggtaactt ctgtttacgc agcatgttga aagattcgcg cagcgatcag
    17581 ttactggcga tggtcgggcc gggactaatg ctttgggcac cgcgagaata cgaactgttc
    17641 cgactaatgg ataattctct ggcggaagat ttgctctgga gttacctgca acgcgcgcca
    17701 gtggcggagt cgttcatctg gcggcgttgg ttgtatgtgt tatgggatga agttgcgcaa
    17761 ctggttaata ccggacgatt tagtcggcgc aacttcgatc tggcatcaaa atcactcttg
    17821 ccgtggctcg cctgacgaac cttttagcca ctgccagatg cggccaagcg tttcatagcc
    17881 aacacgatcg ctatgcatca accagaccgg agaagggatt gcccgttccc acgggttaag
    17941 cggcgagtcg atggccaact gattcgccgg ggctggtagt ggatttaacc cttcctgctg
    18001 aaaaaagatc atcgcccgcg gcagatgtga tgcggaggtc accagcagga aaggggcgtc
    18061 accaatcgcc tgtttcactg ccgcagcttc ttcttcggta tcttttggca aatccagggt
    18121 gataatttgc tcgcgcggta cgcccagcga ttgcgcaact ctggcaccta cttccgctgt
    18181 actcaccgta ttggtttttg ctacgcctcc cgtgaagatc agttttgaac ccggattttc
    18241 gcgccataaa cggataccct cattcaggcg tggcagactg ttattgatta aattagagct
    18301 cggtgcccac tgcgggttcc aggtataacc acctccgagc accacgatat agtccacttt
    18361 ttgcgaatta ttccatgtcg gataggtgct ttcgatggga cgcagcaggc ggtcggctac
    18421 cggttgcaaa ctcaataaca agagtgccag ccacccaatg ctaataaaaa tcttcccggt
    18481 tttttgaaaa cggctaaacc acagtagcgc caggccagcg ccaataatta acaacatcag
    18541 cggaagtggc agcaacatat tgccaatcac ttttttcagt gtaaaaagca tcctttctgg
    18601 ttcctttttt aaccatatag caagggatcg ccagcgatat tacaccagac gggttcattc
    18661 tcgccgtggc tgtgacaaaa tagcgtcttt atcagaaaac gtcttagcca accggtggag
    18721 aaagcaatgc aggatcgcaa ttttgatgat attgcggaaa agttttcccg taacatttac
    18781 ggcaccacca aagggcagct tcgacaggct attctgtggc aggatctcga tcgcgtgctg
    18841 gcggaaatgg gtccgcaaaa actgcgtgtg ctggatgcgg gcggtggaga agggcagacc
    18901 gcaatcaaaa tggccgagcg cgggcatcag gtcattttat gcgatctttc tgcgcagatg
    18961 atcgaccgcg caaaacaggc ggcagaagca aaaggtgtga gcgacaacat gcaatttata
    19021 cattgcgccg ctcaggatgt tgcttcgcat ttggaaacgc ccgttgatct gatattgttc
    19081 catgcggtgc tcgagtgggt ggctgatccc cgcagcgtat tgcagaccct gtggtcagtg
    19141 ttgcgtccag gcggcgtgtt gtcgttaatg ttctacaatg cgcatggttt gttgatgcat
    19201 aacatggtcg ccgggaattt tgattacgtg caggcgggaa tgccgaaaaa gaaaaaacgg
    19261 acgctttcgc cagattatcc acgcgacccg acgcaggttt atctgtggct ggaagaagct
    19321 ggttggcaaa ttatgggtaa gacaggcgtt cgcgtgtttc atgattatct gcgcgagaag
    19381 caccagcagc gcgactgcta tgaagcatta cttgaattag aaacgcgtta ttgccgtcag
    19441 gaaccgtata ttaccctggg gcgttatatt catgtcaccg cgcgcaaacc gcagagcaag
    19501 gataaagtat gagtgaattt tcccagacag tccccgaact ggttgcctgg gccagaaaaa
    19561 atgacttctc catttcgctg ccggtagacc gactctcttt tctgctggca gttgccacgc
    19621 tgaacggcga gcgtctggat ggtgagatga gtgaaggcga gctggtggat gcattccgcc
    19681 atgtgagtga tgcgtttgag caaaccagcg aaaccatcgg cgtgcgcgcc aacaacgcga
    19741 tcaacgacat ggtgcgtcaa cgtctgctga accgctttac cagcgagcag gcagaaggga
    19801 acgcaattta ccgtctgacg ccgctcggca tcggcattac tgactattac atccgtcagc
    19861 gcgagttttc tacgctgcgc ctttctatgc agttgtcgat tgtggcgggt gagctcaaac
    19921 gcgcagcaga tgccgccgaa gagggcggtg atgaatttca ctggcaccgt aatgtctatg
    19981 cgccactgaa atattcggta gcagaaattt tcgacagtat cgacctgacg caacgtctga
    20041 tggacgaaca gcagcagcag gtgaaggacg atatcgccca gttgctgaac aaagactggc
    20101 gggcggcgat ttccagctgt gaattgttgc tttcggaaac ttccggaacg ctgcgtgaat
    20161 tgcaggatac actggaagcg gcaggcgaca aattgcaggc taatctgttg cgcattcagg
    20221 atgcgacgat gactcatgac gatctgcatt tcgtcgatcg tctggtgttc gatctgcaga
    20281 gcaaactcga ccgtattatc agttggggcc agcaatccat cgatttgtgg attggctacg
    20341 accgccacgt acataaattt attcgtaccg cgatcgatat ggataaaaac cgcgtctttg
    20401 ctcagcggtt acgtcagtcg gtacaaacct attttgatga gccgtgggcg ctaagttatg
    20461 ccaatgccga tcgtctgctg gatatgcgtg acgaagagat ggcactgcgc gatgaagaag
    20521 tgactgggga acttccgcca gatttggaat acgaagagtt taacgagatc cgcgaacagc
    20581 tggcggcgat catcgaagaa caacttgccg tgtacaaaac cagacaagtg ccgctggatc
    20641 ttggtctggt ggtacgcgaa tatctgtcac agtatccgcg tgcacgtcac tttgacgttg
    20701 cgcgcattgt tattgatcag gcggtacgtc ttggcgtagc gcaagcagat ttcaccggac
    20761 tgccagcgaa atggcagccg attaatgatt acggagccaa ggtacaggcg catgtcatcg
    20821 acaaatattg aacaagtgat gccggttaag ctggcgcagg cgctggcgaa tccgttattt
    20881 ccggcgctgg acagcgcctt acgttcaggc cgccatattg gcctcgacga actggataat
    20941 catgcattcc tgatggattt tcaggaatat ctggaagagt tttacgcgcg ttataacgtt
    21001 gagcttattc gtgcaccgga agggttcttc tatttacgtc cacgttccac cacgctgatc
    21061 cctcgttccg tcttgtcgga actggatatg atggtcggga aaatcctctg ttatctctat
    21121 ctcagcccgg aacggctggc gaatgagggg attttcaccc agcaggaact gtacgacgaa
    21181 ctgctcaccc tggccgatga agcgaaactg ctgaaactgg tgaacaaccg ttcaaccggt
    21241 tcagacattg accgtcagaa gttgcaggaa aaagtacgtt cttcgctcaa ccgtctgcgt
    21301 cgtttaggta tggtgtggtt tatgggccac gacagcagca agttccgcat taccgaatcg
    21361 gtgttccgct tcggagccga tgtgcgtgct ggcgacgatc cccgtgaagc acagcgtcgc
    21421 ctgattcgtg atggcgaagc aatgccgatt gaaaatcatc tgcaactcaa cgatgaaacc
    21481 gaagagaatc agccagatag cggagaggaa gaataatgat tgaacgcggt aaatttcgct
    21541 cactgacgct gattaactgg aacggcttct ttgcccgaac ttttgacctt gacgagctgg
    21601 tcacgacgct ttctggcggt aacggggcgg gtaaatccac caccatggcg gcgttcgtta
    21661 cggcgctgat ccccgacctg accctgctgc atttccgtaa cactacggaa gccggggcca
    21721 ccagcggttc gcgcgataaa ggtctgcacg gtaagctgaa agcgggtgtc tgttattcga
    21781 tgctcgacac cattaactcg cgccaccagc gcgtggtggt cggtgtgcgt ctgcaacagg
    21841 ttgccggacg cgatcgtaaa gtggatatca aaccatttgc cattcaggga ctgccgatgt
    21901 cggtgcagcc gacacagctg gtgaccgaaa ctctgaacga acgccaggcg cgcgtgctgc
    21961 cgcttaacga gctgaaagac aagctcgagg cgatggaagg cgtgcagttt aaacagttca
    22021 actccattac cgattaccac tcgctgatgt tcgatctggg catcattgcg cgtcgtctgc
    22081 gctccgcatc tgaccgtagc aaattctatc gtctgatcga agcttcgctg tatggcggga
    22141 tctccagtgc cattacccgt tctctgcgcg actacctgtt gccggaaaac agcggcgtgc
    22201 gtaaagcgtt ccaggacatg gaagcggcgc tgcgtgaaaa ccgcatgacg ctggaagcga
    22261 ttcgtgtcac ccagtcggac cgtgacctgt ttaagcatct gatcagcgaa gctaccaact
    22321 acgtggcggc ggactacatg cgtcacgcca acgagcgccg tgtccatctc gataaggccc
    22381 tggagtttcg tcgcgaacta catacttcgc gtcagcaact ggcggcggag cagtacaaac
    22441 acgtcgatat ggcgcgtgag ctggcagagc acaacggtgc cgaaggcgat ctggaagcgg
    22501 attatcaggc ggccagcgat cacctgaacc tggtgcaaac cgcactgcgt cagcaggaga
    22561 agatcgaacg ctacgaagcg gatctcgatg agctgcagat ccgtctggaa gagcaaaacg
    22621 aagtggtggc agaagccatc gaacgccagg aagagaacga ggctcgcgcg gaagctgccg
    22681 aactggaagt ggacgagctg aaaagccagc ttgctgacta ccagcaggcg ctggacgtcc
    22741 agcaaacgcg cgcgatccag tacaaccagg cgattgctgc gcttaatcgt gccaaagaac
    22801 tgtgccatct gccggactta accgccgaca gcgccgccga atggctggaa accttccagg
    22861 cgaaagagct ggaagcgact gaaaaaatgc tctctcttga gcagaaaatg agcatggcgc
    22921 aaaccgcgca cagccagttt gagcaggctt atcagctggt ggtggcaatc aacggcccgc
    22981 tggcgcgtaa cgaggcgtgg gatgtcgctc gcgaactatt gcgcgaaggg gtcgatcagc
    23041 gtcacctggc agagcaggtt cagccgttgc ggatgcgctt aagcgaactg gaacagcgtc
    23101 tgcgcgaaca gcaagaagct gagcgtctgc tggcagattt ctgcaaacgt cagggcaaga
    23161 attttgacat tgacgagctg gaagccctgc atcaggagct ggaagcacgc attgcctctc
    23221 tttccgatag cgtgtctaac gcccgtgaag agcggatggc actgcgccag gagcaggaac
    23281 agctgcagtc tcgcattcag agtttgatgc agcgtgcacc ggtttggctg gcagcgcaaa
    23341 acagtctcaa ccagttgagc gaacagtgcg gcgaagagtt cacctccagc caggacgtca
    23401 ctgaatatct gcaacagttg ctggagcgtg agcgcgaggc cattgttgaa cgcgatgaag
    23461 taggcgcgcg caaaaacgcc gtcgatgaag agatcgaacg tttaagccag cctggcggct
    23521 ctgaagatca gcgtctgaac gcgctagcgg agcgttttgg tggtgtgctg ctgtcagaaa
    23581 tttatgacga cgttagcctg gaagatgcgc cgtacttctc agcgctgtat ggcccgtcac
    23641 gccacgccat cgtggtgcca gatctgtcac aggtaaccga acacctggaa ggtttgaccg
    23701 attgcccgga agatctctat ttgatcgaag gggatccgca gtcattcgat gacagcgtgt
    23761 tcagcgttga tgagctggaa aaagcggtag tggtgaaaat cgccgatcgt cagtggcgtt
    23821 attcacgttt cccggaagtg ccgctgtttg gtcgtgctgc gcgtgaaagc cgtattgaaa
    23881 gcctccatgc cgagcgtgaa gtgctttccg aacgcttcgc cacgctctcc tttgatgtac
    23941 agaaaaccca gcgtctgcat caggcgttca gccgctttat cggcagtcat ctggcggtgg
    24001 cgtttgagtc tgacccggaa gcagaaatcc gtcaactgaa cagccgtcgc gtcgaactgg
    24061 agcgggcgtt gagtaatcat gaaaatgata accagcagca gcgtattcag tttgagcagg
    24121 cgaaagaagg cgttacggcg ctgaaccgca ttctgccgcg tctcaacctg ttggctgatg
    24181 acagcctggc ggatcgcgtc gatgaaatcc gcgaacgtct ggatgaagcc caggaagccg
    24241 cgcgttttgt tcagcagttt ggcaatcaac tggcgaaact ggaaccgatc gtttcggtat
    24301 tgcagagcga cccggaacag ttcgaacagt taaaagaaga ttacgcgtac tctcagcaga
    24361 tgcagcgcga tgcccgtcag caggcgtttg ccctgacgga agtggtgcag cgtcgtgcgc
    24421 actttagcta ctctgactcg gcagaaatgc ttagcggtaa cagcgatctc aacgaaaaac
    24481 tgcgtgaacg tctggaacag gcggaagcgg agcgtacccg cgctcgcgaa gcgttgcgcg
    24541 gtcacgcagc gcagttgagt cagtacaacc aggtgctggc ttcgctgaaa agttcttacg
    24601 acaccaaaaa agagctactc aatgatctgc aacgtgaatt gcaggatatc ggcgtgcgtg
    24661 ctgatagcgg ggcagaagag cgggcacgta ttcgccgtga cgagctgcat gcacaactga
    24721 gcaataaccg ttcgcgccgc aatcaactgg aaaaagcgct caccttctgc gaagcggaga
    24781 tggataacct gacccgcaaa ctgcgcaagc tggagcgtga ttactttgag atgcgcgagc
    24841 aggtagtgac cgcgaaagcg ggctggtgtg cagtcatgcg catggtgaaa gataacggcg
    24901 ttgagcgccg cttacaccgt cgtgagctgg cttatctctc cgctgatgat ttgcgttcca
    24961 tgtcggataa ggcgttaggt gcgctgcgtc tggcggtggc ggataacgaa catctgcgcg
    25021 acgtgctgcg catgtcggaa gatcccaaac gtccggagcg taaaattcag ttcttcgtgg
    25081 cggtttatca gcatctgcgt gaacgtattc gtcaggatat tattcgtacc gatgatccgg
    25141 tggaagctat cgaacagatg gagatcgaac ttagccgtct gaccgaagag ttaacctccc
    25201 gtgaacagaa actggcgatc agttcccgca gcgtggcgaa catcattcgc aaaaccattc
    25261 agcgcgagca gaaccgtatc cgtatgctca accaggggtt gcagaacgta tcgtttggtc
    25321 aggtgaacag cgtgcgcctc aacgtgaacg tgcgtgaaac gcacgccatg ctgctggatg
    25381 tgctctctga acagcatgag cagcatcagg atctgtttaa cagcaaccgt ctgaccttct
    25441 cggaagcgct ggcgaaattg tatcagcgtc ttaatccgca gattgatatg gggcagcgca
    25501 cgccgcagac cattggcgaa gaactgctgg attaccgcaa ctatctggaa atggaagttg
    25561 aggttaaccg tggttccgat ggctggttgc gcgcagagtc tggtgcattg tcgaccggtg
    25621 aggcgattgg taccggtatg tcgattctgg tgatggtggt acaaagctgg gaagatgaat
    25681 ctcgccgcct gcgcggtaaa gatatctctc cttgccgcct gctgttcctc gatgaagcag
    25741 cgcgactgga tgctcgttct atcgccacgc tgtttgaatt gtgtgagcgt ttgcaaatgc
    25801 agctcatcat cgcagcgccg gaaaatatca gcccggagaa aggcaccacc tataaactgg
    25861 tgcgtaaagt cttccagaat accgaacatg ttcatgtcgt cggcctgcga ggatttgcgc
    25921 cgcaactccc tgaaacgctt ccaggaagtg acgaagcgcc ttctcaggcg agttaaaatt
    25981 aaggcggcag caatgccgcc ttttcttttt ccgaaaactc cgtttctgca ctaaaaaagt
    26041 ggcacattac ggcgcgcttt tctttaaact tctttacatt aggttatgta aaaacgtatc
    26101 ggcgtttata tactgaagat aagcctgatg agtaacaggc ttgctcgtca tactttcgtg
    26161 agtattggcg ttgtacaggc aagtcgtaaa ataacagcct ggctattcag agtatgataa
    26221 aaacaggggg caagggatgt tgcttaatat gatgtgtggt cgtcggctgt cggcaatcag
    26281 tttgtgcctg gccgtaacat tcgctccact gttcaatgcg caggccgatg agcctgaagt
    26341 aatccctggc gacagcccgg tggctgtcag tgaacagggc gaggcactgc cgcaggcgca
    26401 agccacggca ataatggcgg ggatcctgcc attgcctgaa ggtgcggcag aaaaagcccg
    26461 cacgcaaatc gaatctcaat tacccgcagg ttacaagccg gtttatctta accagcttca
    26521 actgttgtat gccgcacgcg atatgcaacc catgtgggaa aaccgtgatg ctgttaaagc
    26581 cttccagcaa cagctggcag aggtggcgat tgccggtttc cagccgcagt ttaataaatg
    26641 ggtagagtta ctgaccgatc ctggtgttaa cgggatggca cgcgacgtgg tgctctctga
    26701 tgcgatgatg ggctatctcc atttcattgc aaatattccg gtcaaaggca ctcgctggct
    26761 atatagcagt aaaccttatg cgcttgcaac gccgccgctt tcggtgatta accaatggca
    26821 gcaggcgctg gataaaggtc aattgcctac gtttgttgca ggactggcac cgcagcatcc
    26881 gcaatatgcg gtgatgcatg aatcgttact ggccttactc agtgacacca aaccgtggcc
    26941 ccaactgacc ggcaaagcaa cgttgcgccc agggcagtgg agtaacgacg taccggcgtt
    27001 gcgtgaaata ttgcaacgca caggcatgtt ggacgggggg ccgaaaatta ctctacctgg
    27061 cgatgacacg ccaactgacg cggtagtcag cccatccgct gttactgttg aaacagcaga
    27121 aactaagccg atggataagc aaacgacgtc tcgtagtaaa cctgcgcctg ccgttcgcgc
    27181 cgcctacgat aatgaactgg tggaagccgt taaacgtttt caggcatggc aaggattggg
    27241 ggcagatggt gctattggcc cggcaacgcg tgactggtta aacgtaacgc ccgcccagcg
    27301 tgctggcgtg ttggctctca acatccagcg attgcgcttg ctgccaacag agctttctac
    27361 cgggatcatg gttaacattc cggcctattc gctggtctac tatcagaacg gcaatcaggt
    27421 gctggattcg cgagtcattg tcggtcgccc cgatcgcaaa acgccgatga tgagcagtgc
    27481 ccttaacaat gtagtggtaa acccgccgtg gaacgtacct ccaactctgg cacgcaaaga
    27541 tattctgccg aaagtgcgca acgatccggg atatctcgaa agccatggct atacggtgat
    27601 gcgcggctgg aacagcagag aagcgattga cccatggcag gttgactggt ctacaatcac
    27661 ggcctcgaat ttaccgtttc gcttccaaca ggctccaggc ccacggaact cgctggggcg
    27721 ctataaattc aatatgccga gttcagaggc catttatttg catgacacgc cgaaccacaa
    27781 tctgttcaag cgtgatacac gcgcattgag ctcaggctgt gtacgagtga ataaagcttc
    27841 cgatctggcg aatatgctgt tgcaggatgc aggctggaat gacaaacgta tttctgatgc
    27901 gctgaagcag ggtgatacac gttacgtcaa tattcggcag tcgattccgg tgaatctcta
    27961 ctacctgacg gcctttgttg gtgcagatgg tcgtacccag tatcgtacag atatttacaa
    28021 ttatgatctg cctgcgcgat ccagctcgca aatcgtatcg aaagcggaac aattaatcag
    28081 gtaaatgaag tagttcgggg aaatcagttg tcgtaataat tagcatgatt gggggcaatt
    28141 ctctgcagcc cccgtcactg ctggggttga gtcatcttga cgtctgcttt acgggcggtt
    28201 aaggtgcctc ttgtgcgcca gaagtgcata taaacgataa cattgacctg tagacttgat
    28261 tatcatggac aaattcgacg ctaatcgccg caaattgctg gcgcttggtg gcgttgcact
    28321 cggtgccgcc atcctgccga cccctgcgtt tgcaacactc tctaccccac gcccgcgcat
    28381 tttgacactc aataatcttc ataccggaga gtcaatcaaa gcggagtttt tcgatggcag
    28441 aggctatatt caggaagaat tggcaaaact taaccatttt ttccgcgatt accgcgcgaa
    28501 caaaataaag tccatcgacc caggattatt cgaccagttg tatcgcctgc aagggttatt
    28561 aggcacccgc aaaccggtgc aactcatttc cggttatcgt tccattgata ccaacaatga
    28621 actacgcgcc cgcagccgtg gagtagcgaa gaaaagctat cacactaaag gccaggcgat
    28681 ggatttccat attgaaggta tcgcgttaag caatattcgc aaagccgcgt tatctatgcg
    28741 cgcaggtggt gtaggatatt acccacgtag taactttgtg catattgata ccgggccagc
    28801 acggcactgg tagtaattgc ttaacgaaac aggggcagta tgaactatcg tattattccg
    28861 gtcaccgcat tctcccagaa ctgttcattg atctggtgtg aacaaacccg cctggccgca
    28921 ctggtcgatc ctggcggcga tgcggaaaaa atcaaacagg aagttgacgc cagcggcctg
    28981 acactgatgc agatcctgct gacgcatggt catctggacc acgttggcgc agcggcggaa
    29041 ctggcgcaac attacggcgt gccggttttt ggcccggaaa aagaagatga gttctggctg
    29101 caaggcttgc ctgcgcaaag tcgtatgttt ggtctggaag agtgccagcc gctgacgcca
    29161 gatcgttggc tgaacgaagg cgataccatc agcataggga atgtgacttt acaggtgtta
    29221 cattgccctg ggcatacgcc gggtcatgtc gtgttttttg atgatcgggc aaagctgctg
    29281 atttctggcg atgttatttt caaaggcgga gtagggcgca gtgacttccc gcgtggcgat
    29341 cataatcaac tgatttcttc aatcaaagat aaattgctgc cactgggtga tgacgtgaca
    29401 tttattccgg gtcacggacc attatccaca cttggttatg aacgcctgca taatcccttc
    29461 ctgcaagacg aaatgcccgt ctggtaaggc acacataaaa aagcccgctt ttcagcgggc
    29521 ttcattgttt ttaacgctta cagcactgcc acaatcgctt cgcacagcgg agccatgtta
    29581 tctggtgtca tcccggccac gtttacgcga ccagaagcaa cagcatacac gccaaactct
    29641 tcgcgcagac gcagcacttg ttcttttgtc aggccactga aggagaacat gccgttctgt
    29701 ttgatgataa agctgaagtc gcggtttgcg cctttttcct gcagcgtatt gacgaacaac
    29761 tgacgcatac gctgaatacg ctggcgcata tcagtcagct cttgttccca aatcgcacgt
    29821 aacgcatcgt tgctcaggat ggtggcaaca acagaagcgc cgtgtgctgg tgggttagag
    29881 tagttagcgc gaatcgccgc tttcatttgg ctgaatgcgc gatcaacggt ttcactgtcg
    29941 gcagcaacca gagtacaagc gccaacacgc tcgttgtaca ggccaaagtt tttagagtag
    30001 gaactggcaa caatcagctc tttatgcata gccgcgaaag cgcgcagtcc ttcagcatct
    30061 tcttccagac cacgggcaaa accctggtaa gcgaagtcaa acagcggtaa ccagcctttc
    30121 tcaacggaga gttgtgccag tgtctgccat tgttccagcg tagggtcgat accggttggg
    30181 ttgtggcagc agccatggaa cagcactacg tcgccagcct gagcttcgtt caggctgtta
    30241 atcagtgcat cgaagtcaag ggtgtggttt tccgcatcat aataagcgta ttcacgaact
    30301 tccagatctg cagagttaaa gacgctctta tggttcggcc agcttgggtt gctcacccac
    30361 actcgcttaa cgctggtatt ttttgccagg aaatcggcag ctatgcgtag tgcgccagtg
    30421 ccacccggag tctgtgccgt gcgagcacgt ttgtcattga tcagggcgct acctttacca
    30481 aacagcagtt cctgagtgca gcgaccaaat tcagggatgc cgtcaatgcc gaggtaattt
    30541 ttggtggttt cattttcgag cagatactgt tcagcctttt tcacgctggt cagtaccggg
    30601 gttttgcccg tctcatcttt atagacacca atcccgaggt taattttgcc gggacgttca
    30661 tcggcacgaa acagatcggc caggcccaga atcgggtcgg caggagcggc ggtaatgttc
    30721 tcaaacatga cgaggttcca ttatggttac agaagggaag tccgctatca gggtaacggg
    30781 agatttacaa aattccaact attactgatg aaaacgcagg ctgtttttgc aagacgtgag
    30841 attgctctgg aaggcataaa aaaaacagga ccaaagtcct gttttttcgg catttaacaa
    30901 agaggtgtgc tattagaact ggtaaacgat acccacagca acggtgtcgt ctgaacctac
    30961 gcccagtttg ttgtcagaat cgatctggtt gatgatgtag tcaacatagg tggacatgtt
    31021 tttgttgaag tagtaggttg cgcccacttc aaagtagttc accagatcaa catcaccgat
    31081 accttctacg tctttcgctt tagatttggt gtaagcgatg gacggacgca gaccgaaatc
    31141 gaactggtat tgcgcaacta acagaacgtc ttgcgttttg ttggcgaagc cgctgatgtt
    31201 tgtaaattta ttagtgatcg gcgtagcgtt acgcgtttca ccgtagttcg ctgccaggta
    31261 gatgttgttc gcatcatact tcagaccagt agcccactgt tcagcttttt taccgttgcc
    31321 aagaagttga gcttcttgca ggttggtacg gtcagctgca ccataagcac caacgatacc
    31381 aaagccttcg tattcgtagc tgatagaacc gccaacaccg tcgccgttag agcggcgtgc
    31441 agtgtcacgc tcgtttttac ccaggtactg aacagcgaag ttcaggccat caaccagacc
    31501 aaagaagttg gagttacgat aggtagcaac gccgccaaca cgaccaacga agaagtcatc
    31561 gctgtatgca gtatcaccac caaattctgg cagcatatcg gtgtaaccca gtgcatcata
    31621 aaccacaccg tagttacggc cgtaatcgaa agaaccaacg tcagcgtatt taagacccgc
    31681 gaatgccaga cgcgttttgt taccagtttg agcgtcagcg ccttcagagt tgttaccctg
    31741 gaagttatat tcccactgac cataaccggt cagatcggaa ttgatttgag tttccccttt
    31801 aaaaccaaga cgggcatagg tcatgtcgcc attgccaccg taactgtttt caccgttacc
    31861 cttggagaaa taatgcagac cgacagcttt accgtacaga tctactttgt tgccatcttt
    31921 gttatagatt tctgcagcgt ttgcagtacc tgctactaac agagcaggga cgatcactgc
    31981 cagaatattg cgcttcatca ttatttatta ccctcatggt tttttttatg acacctgcca
    32041 ctgccgtcaa taatttctgt caataaaatt ttacggaact attgatgaga gtttggtgtc
    32101 tttatgtgtc tgcaggcatc tttccattca aactaacgtt t
//
LOCUS       DAZKIP010000044        31986 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZKIP010000044 DAZKIP010000000
VERSION     DAZKIP010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.044"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..234)
                     /locus_tag="ACHOUK_002283"
     CDS             complement(<1..234)
                     /locus_tag="ACHOUK_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9897733.1"
                     /translation="MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVI
                     KKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSK"
     gene            complement(460..1785)
                     /gene="rimO"
                     /locus_tag="ACHOUK_002284"
     CDS             complement(460..1785)
                     /gene="rimO"
                     /locus_tag="ACHOUK_002284"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFP9897734.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            1998..2381
                     /gene="bssR"
                     /locus_tag="ACHOUK_002285"
     CDS             1998..2381
                     /gene="bssR"
                     /locus_tag="ACHOUK_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFP9897735.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            2492..3607
                     /gene="yliI"
                     /locus_tag="ACHOUK_002286"
     CDS             2492..3607
                     /gene="yliI"
                     /locus_tag="ACHOUK_002286"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFP9897736.1"
                     /translation="MHRQFFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(3604..4230)
                     /gene="gstB"
                     /locus_tag="ACHOUK_002287"
     CDS             complement(3604..4230)
                     /gene="gstB"
                     /locus_tag="ACHOUK_002287"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFP9897737.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            4476..5678
                     /gene="dacC"
                     /locus_tag="ACHOUK_002288"
     CDS             4476..5678
                     /gene="dacC"
                     /locus_tag="ACHOUK_002288"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFP9897738.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            complement(5725..6483)
                     /gene="deoR"
                     /locus_tag="ACHOUK_002289"
     CDS             complement(5725..6483)
                     /gene="deoR"
                     /locus_tag="ACHOUK_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFP9897739.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(6541..7137)
                     /gene="ybjG"
                     /locus_tag="ACHOUK_002290"
     CDS             complement(6541..7137)
                     /gene="ybjG"
                     /locus_tag="ACHOUK_002290"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFP9897740.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLLVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            7422..8659
                     /gene="mdfA"
                     /locus_tag="ACHOUK_002291"
                     /pseudo
     CDS             7422..8659
                     /gene="mdfA"
                     /locus_tag="ACHOUK_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            complement(8700..8984)
                     /gene="ybjH"
                     /locus_tag="ACHOUK_002292"
     CDS             complement(8700..8984)
                     /gene="ybjH"
                     /locus_tag="ACHOUK_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFP9897741.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYHPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(9070..9885)
                     /gene="ybjI"
                     /locus_tag="ACHOUK_002293"
     CDS             complement(9070..9885)
                     /gene="ybjI"
                     /locus_tag="ACHOUK_002293"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFP9897742.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKMVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKTLHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGNAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(9885..11093)
                     /locus_tag="ACHOUK_002294"
     CDS             complement(9885..11093)
                     /locus_tag="ACHOUK_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9897743.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALVGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSMVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            11177..11713
                     /gene="rcdA"
                     /locus_tag="ACHOUK_002295"
     CDS             11177..11713
                     /gene="rcdA"
                     /locus_tag="ACHOUK_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFP9897744.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(11888..13573)
                     /locus_tag="ACHOUK_002296"
     CDS             complement(11888..13573)
                     /locus_tag="ACHOUK_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFP9897745.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            13843..14220
                     /locus_tag="ACHOUK_002297"
     CDS             13843..14220
                     /locus_tag="ACHOUK_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFP9897746.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(14250..14507)
                     /gene="grxA"
                     /locus_tag="ACHOUK_002298"
     CDS             complement(14250..14507)
                     /gene="grxA"
                     /locus_tag="ACHOUK_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFP9897747.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            14667..14954
                     /locus_tag="ACHOUK_002299"
     CDS             14667..14954
                     /locus_tag="ACHOUK_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFP9897748.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            14938..15660
                     /gene="nfsA"
                     /locus_tag="ACHOUK_002300"
     CDS             14938..15660
                     /gene="nfsA"
                     /locus_tag="ACHOUK_002300"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFP9897749.1"
                     /translation="MTPTIELTCGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPSSILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            15721..16623
                     /gene="rimK"
                     /locus_tag="ACHOUK_002301"
     CDS             15721..16623
                     /gene="rimK"
                     /locus_tag="ACHOUK_002301"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFP9897750.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            16711..17187
                     /locus_tag="ACHOUK_002302"
     CDS             16711..17187
                     /locus_tag="ACHOUK_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFP9897751.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            17538..18650
                     /gene="potF"
                     /locus_tag="ACHOUK_002303"
     CDS             17538..18650
                     /gene="potF"
                     /locus_tag="ACHOUK_002303"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFP9897752.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQAPKIDRVRTRAWTKVKSGK"
     gene            18745..19878
                     /gene="potG"
                     /locus_tag="ACHOUK_002304"
     CDS             18745..19878
                     /gene="potG"
                     /locus_tag="ACHOUK_002304"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFP9897753.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLYSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            19888..20841
                     /gene="potH"
                     /locus_tag="ACHOUK_002305"
     CDS             19888..20841
                     /gene="potH"
                     /locus_tag="ACHOUK_002305"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFP9897754.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNFGNFLQLTGDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            20838..21683
                     /gene="potI"
                     /locus_tag="ACHOUK_002306"
     CDS             20838..21683
                     /gene="potI"
                     /locus_tag="ACHOUK_002306"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFP9897755.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAVISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     CARRG"
     gene            21743..22231
                     /locus_tag="ACHOUK_002307"
     CDS             21743..22231
                     /locus_tag="ACHOUK_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFP9897756.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            22272..23399
                     /gene="rlmC"
                     /locus_tag="ACHOUK_002308"
     CDS             22272..23399
                     /gene="rlmC"
                     /locus_tag="ACHOUK_002308"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFP9897757.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQA
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALVERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIALEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKM"
     gene            23679..25022
                     /locus_tag="ACHOUK_002309"
     CDS             23679..25022
                     /locus_tag="ACHOUK_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000399308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP9897758.1"
                     /translation="MEGVPTMFAKFIDVIQTFLTEPAILIGLLVGIGYALDKKSPIKI
                     ITGMVSAMVGLMMVLFGGFQFSATFKPVAEAVSKAYGVHGYLMDSYAMKAATQIALGD
                     NFGYVGYVFVLAFFTNLLLVLFGRYTGAKGIFLTGNTGVSHSQAVLWLIVFWLGFSWT
                     TSIIIAGILTGVFWAFSTTLIVKPIAKVTKDAGFTIAHNQMLGLWFFSKFAHKFGDPE
                     KHDAENLKLPGWLAIFNHNVTAIAIVMTLFVGGFLLSTGIDNVQLMAKGKPWYIYIIN
                     LGLQFSMYMVILLQGVRMMVGEINGSFKGWQDRFIPNAIPAVDVAALLPFSPNAATLG
                     FVFCTFGTIFSMGILLLVHSPIMVLPGFVPLFFSGGPIGVLANRMGGYRSVIICTFLL
                     GIIQTFGTVWAIPLTGLAENGVGWTGIFDWATVWPAICEVLKFIAATFHLGPYAG"
     gene            25044..25364
                     /locus_tag="ACHOUK_002310"
     CDS             25044..25364
                     /locus_tag="ACHOUK_002310"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9897759.1"
                     /translation="MKDTINILFVCGYGVGSSVMLQTVVKKALTKYDFKVEMEHTAAG
                     EVGGFTDWADIFAISKKLLDVVSLDPKQGQYLIPIENIMDGETIGQQIYEVVQQHFPH
                     LINQ"
     gene            25376..26857
                     /locus_tag="ACHOUK_002311"
     CDS             25376..26857
                     /locus_tag="ACHOUK_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HFP9897760.1"
                     /translation="MRAIILLFDSLNKRYLPPYGDALTKAPNFQRLAAHAATFENSYV
                     GSMPCMPARRELHTGRCNFLHREWGPLEPFDDSMPELLKKAGIYTHLISDHLHYWEDG
                     GGNYHNRYSSWEIVRGQEGDHWHASVAQPPIPEVLRVPQKQTGGGVSGLWRHDWANRE
                     YIQQEADFPQTKVFDAGCAFIHKNHAEDNWLLQIETFDPHEPFHTTEEYLSLYEDNWD
                     GPHYDWPRGRVQESDEAVEHIRCRYRSLVSMCDRNLGRILDLMDEHDLWRDTMLIVGT
                     DHGFLLGEHGWWAKNQMPYYNEVANNPLFIWNPRSGVKGERRQALVQMIDWAPTLYDF
                     FQQPVPPDVQGQPLAKTVSHDEPVRSSAMFGVFSGHANVTDGRYVYMRAALPGREDDI
                     ANYTLMSCKMNSRYPVDEMRALSLAPPFRFTKGLQVLRIPAQEKYKGLNQFGHLLFDL
                     QNDPQQLHPIHDDVIESRMIALLIQLMKDNDAPPEQFQRLGLA"
     gene            complement(26908..27639)
                     /gene="artJ"
                     /locus_tag="ACHOUK_002312"
     CDS             complement(26908..27639)
                     /gene="artJ"
                     /locus_tag="ACHOUK_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFP9897761.1"
                     /translation="MKKLVLAALLASCAFGASAAEKINFGVSATYPPFESMDANNQIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPDRSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNSRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQQISDQWFPQ"
     gene            complement(27930..28598)
                     /gene="artM"
                     /locus_tag="ACHOUK_002313"
     CDS             complement(27930..28598)
                     /gene="artM"
                     /locus_tag="ACHOUK_002313"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFP9897762.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(28598..29314)
                     /gene="artQ"
                     /locus_tag="ACHOUK_002314"
     CDS             complement(28598..29314)
                     /gene="artQ"
                     /locus_tag="ACHOUK_002314"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFP9897763.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGIVEIPVQMQIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPMGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(29321..30052)
                     /gene="artI"
                     /locus_tag="ACHOUK_002315"
     CDS             complement(29321..30052)
                     /gene="artI"
                     /locus_tag="ACHOUK_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFP9897764.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITAEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(30070..30798)
                     /gene="artP"
                     /locus_tag="ACHOUK_002316"
     CDS             complement(30070..30798)
                     /gene="artP"
                     /locus_tag="ACHOUK_002316"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFP9897765.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(31016..31531)
                     /locus_tag="ACHOUK_002317"
     CDS             complement(31016..31531)
                     /locus_tag="ACHOUK_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP9897766.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYHIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
BASE COUNT         7839 a         7775 c         8260 g         8112 t
ORIGIN      
        1 cttggaccaa ccatcataaa aaaacacatg atttgctgat ttaaaaaaga taattgttct
       61 ggctccacca ctctttcctt gttggagggg taaccgtttt ttgatgacac cgctacctaa
      121 atcagcctcc catatgcctt gcatcacttc ttcagcggca gccatgagaa ctttatcgtg
      181 aataccaagt tttttcgtgc tttggctgaa tcgccttgcc ttatacacac ccatacatcc
      241 atccttgtgg taatgtttac tatagtaacc attacgctta tttcaagtaa ttgcgatcaa
      301 aactttaatt tggtgtgaaa aagtgagagt tgcctcgagt acgattcatt tttctcgatt
      361 tatgcgtcag gggattttgt gcgtaagggt aggacatcgg gaagggtagt agaggcagat
      421 cgaagaacaa tatccttgtt agatctgcct ttagattaat taaacccggc taccccacaa
      481 atcgtactca tcggcgtgct cgactttcac acgcaggata tcacccggct taacgttggt
      541 ttcaccattg agataaaccg caccgtcgat ttccggtgca tctgccatgc tgcgaccaat
      601 cgcgccttct tcgtccactt cgtcgataat caccagaatt tcacggccca ctttctcttg
      661 caggcgctcg gcggaaatct gctgctgcaa ctgcatgaaa cggttccagc gttcttcttt
      721 cacttcttcc ggaacctggt caggcagggc attggcgtct gcaccttcaa ccgggctgta
      781 tttaaagcag ccaacgcgat ccagacgcgc ttctttcagg aagtcgagta gcatctggaa
      841 atcttcttcc gtctcgccag ggaagccgac aataaaggtt gagcgtaggg tcagttccgg
      901 gcagatttcg cgccactgtt tgatgcgcgc cagttggcga tctacagaac ccggacgctt
      961 catcagtttg agaatgcgcg ggctggcgtg ctgcaacgga atgtccagat acggcaggat
     1021 tttgccttct gccatcagtg ggatgacgtc gtccacatgc ggataagggt aaacgtagtg
     1081 cagacgtgtc cagatcccca gtttcgataa ctgttcgcac aggctgacca tgctggtttt
     1141 taccggctcg ccgttgtgga agccagtacg atgtttaaca tcaacgccat aggcggaagt
     1201 atcctgcgag atcaccagaa tctctttaac gcccgcatct accagacgtt tcgcttcact
     1261 taatacttcg ccaatcggac ggctcaccag gtcgccgcgc atagacggaa taatgcagaa
     1321 ggtgcagcgg tgattacagc cttcagaaat tttcagatag gcataatgac gcggcgtcag
     1381 tttgacacct tgttctggca ccaggctcag gaatgggttg tgtttcggtt ttggcacgta
     1441 gtgatgaacg tgctccagaa cctgctcata gctatgaggc ccggtgattt ccagcacttt
     1501 cgggtggact tcgcggatct gatcttcttt tgcccccaga caaccggtca caattacctt
     1561 gccgttttca ttcaacgctt caccaatggc ttccagtgat tcttgtaccg cgctgtcaat
     1621 aaagccgcag gtgttgacga tcaccatgtc cgcatcgtca tagctcggta ccacgtcata
     1681 accttcagtg cggagttcgg tgagaatacg ctctgaatcg acaaggtttt tcggacagcc
     1741 aagggaaaca aagccgattt tcggctgggg agttactttg ctcatagctt aaaaaatatt
     1801 cagttacagg aaaggtcagg gcagggattc tacagagttc tggataaaat ttgtatcgca
     1861 atctcattcg ctggcggagg cgaaggaaat gtaaattttg ttaattcggc gtgaagaatt
     1921 gatcctggac agcattatgc tcaaaaaata gccatactat ttaattgcaa caaggctgga
     1981 aagaggagga tcgaagtatg ttcgttgaca gacagcgaat cgatctgctg aaccggttga
     2041 tcgacgcacg cgttgacctc gccgcatacg tgcaactgag gaaggcaaaa ggatacatgt
     2101 ccgtcagcga aagcaatcat ctacgagata acttttttaa actgaatcgc gaactgcacg
     2161 ataaatcgct gcggttgaat cttcatctgg atcaggaaga gtggagtgct cttcatcatg
     2221 ctgaagaagc attagcgaca gccgcagtat gtttgatgag tgggcaccat gattgcccga
     2281 ctgttattac cgtcaacgcc gataagcttg aaaattgtct gatgagctta acgctgagta
     2341 tccagagcct gcagaagcac gccatgcttg agaaggcctg aaaactaagg gggagaaagc
     2401 gtctccccct tcatgtttaa gtttttgtaa aaatgaattt gttatctcct ccactgacta
     2461 cgctttaagc cagagtcaat ccggaggcgt tatgcatcga caattctttt tccttgtgcc
     2521 ccttatttgt ctttcttccg ctctctgggc ggctcctgca acggtaaatg tcgaagtact
     2581 gcaagacaaa ctcgaccatc cctgggcact ggccttttta cccgataacc acggtatgtt
     2641 aatcactctg cgcggcggcg agttgcgtca ctggcaagca ggaaaaggat tatctgcgcc
     2701 gctttccgga gttccggacg tttgggcgca cgggcagggc ggcctgctgg acgtggtttt
     2761 agcgcctgat tttgctcagt ctcgccgcat ctggttaagt tattccgaag ttggcgatga
     2821 tggcaaagcc ggaactgctg tggggtatgg ccgcttaagt gatgatctct caaaagtgac
     2881 cgactttcgc accgtctttc gccagatgcc aaaactgtct accggcaacc attttggcgg
     2941 gcggctggta ttcgacggta aaggttatct ttttattgct ctgggcgaaa acaatcagcg
     3001 cccgacggcg caggatctgg ataaattaca gggcaaactg gtgcgtctga ccgaccaggg
     3061 cgaaatcccg gatgataatc cttttataaa ggaatccggt gcgcgcgccg agatctggtc
     3121 ttatggcatt cgtaatccgc aaggaatggc gatgaatccg tggagtaatg cactgtggct
     3181 gaatgaacat ggcccgcgcg gtggtgatga aattaatatc ccgcaaaaag gcaaaaacta
     3241 cggctggccg ctggcaacct ggggaatcaa ctattcaggc tttaagatac cggaagcgaa
     3301 aggggagatc gtcgccggga ccgagcaacc tgttttttac tggaaagatt cgcccgccgt
     3361 gagcggcatg gccttctata acagcgataa attcccccag tggcagcaaa aattatttat
     3421 tggcgcgctg aaagataaag atgtcattgt gatgagcgtc aacggcgaca aagtaacaga
     3481 agatggccgt attttaacgg acagagggca gcgaattcgt gatgttcgca ctggacccga
     3541 cggttattta tacgttctca ccgacgagtc cagtggggaa ttacttaaag ttagcccacg
     3601 caattagcta acgggaatca tcaccacttt gcgcactgcg gggcgttcag tgagttgctg
     3661 ataccagcgt tgcagatttg gacgcggtgt ccaggtcagg ccaacattaa acaaattgta
     3721 gatgaacggt gcgatagcga tatcgcccac accaaactcg tcgccagaga accattttac
     3781 ttttgccagt tccgcatcga gcagggcaaa cagggcgtcg cactctttgc aactggcatc
     3841 aatggcggcc tgatcgcgct cttccggtgg tgttctgact aatcccatca ggatcccgcg
     3901 atgagcatta ctgagtgtct ggtttgccca gtccatccat ttttctgctt ccgcacgacg
     3961 tgccggtgag tcgatccaca ggcgtttttg cccgtactgt gccgccagat agcggacaat
     4021 ggcgtttgat tcccaaagaa tgagatcact ttcgtcgtca cgcaacaacg gcaccagccc
     4081 gttagggttc atcgccagaa aatcagcatc gtgatttatc ccaaactcac ggcccgcgag
     4141 aatttgctca taaggtagtt ccagttcttc gagcgtcagc aatacttttt ttacgttggt
     4201 tgaattattc cgaccccaca gcgtaatcat actcacccct tttccgacag gcagccacag
     4261 gctgaacagg actccgatgg tgaagtaaag ttaactttta agcaacagct ggcaaaaaaa
     4321 tgacaccaga agcagcgaag cggcaggata ttgcataaac tttaaaaact ttaccaactt
     4381 acggtttctt taagtttgtg tgtgcgttat taatcaccga acttatcata cggcgatata
     4441 acgtattttt ttgaatggat actcgggtgg catttatgac gcaatactcc tctctccttc
     4501 gtggtcttgc agcgggttct gcatttttat tcctttttgc cccaacggca ttcgcggccg
     4561 aacaaaccgt tgaagcgccg agcgtggatg cgcgtgcatg gattttaatg gattacgcca
     4621 gcggtaaagt gctggcagaa ggcaatgcgg atgagaaact ggatcccgcg agcctgacta
     4681 aaatcatgac cagctatgtg gttgggcagg cgcttaaggc cgataagatt aaactcaccg
     4741 atatggtgac ggtcggtaaa gatgcctggg cgacgggaaa tccggcactg cgtggttcat
     4801 cggtaatgtt cctcaaaccg ggcgatcagg tttcggtggc agacttgaac aaaggtgtga
     4861 ttatccagtc cggtaatgac gcctgtattg cgctggccga ttacgttgcc gggagccagg
     4921 agtcatttat tggtttgatg aatggttatg ccaaaaaact ggggctgacc aacactacct
     4981 tccagacggt gcacggtctg gatgcgccgg ggcagttcag taccgcgcgc gatatggcat
     5041 tgctgggtaa agcattgatc cacgatgtgc cggaagagta cgccattcat aaagagaaag
     5101 agttcacctt caacaaaatt cgtcagccta accgtaaccg tctgctgtgg agcagcaatc
     5161 tgaatgttga tggcatgaag acaggaacca cggcaggcgc gggatataat ctggttgctt
     5221 cggctaccca gggcgatatg cgtttaatct ccgtagtgct gggggcgaaa accgaccgta
     5281 tccgttttaa tgagtctgag aaattattga cctggggttt ccgcttcttt gaaaccgtga
     5341 cgccaattaa acctgatgcc acctttgtga ctcagcgcgt ctggtttggt gataagagcg
     5401 aagtgaatct cggggcaggc gaagcgggct cagtgaccat accgcgtggg cagctgaaaa
     5461 acctgaaggc gagctacacg ttaacggaac cgcagcttac cgcaccgctg aaaaaaggtc
     5521 aggttgtcgg gaccattgat ttccagctta acggtaaatc cattgagcag cgtccgctga
     5581 tcgtgatgga aaatgtggaa gagggcggat tctttggtcg gatgtgggat ttcgtgatga
     5641 tgaaattcca tcagtggttc ggcagctggt tctcttaatc ttctgataac cggatggcgc
     5701 gaaacgtcat ccggttatac gtcattaata catcaactta atacgctgcg tctgcgcgta
     5761 cttcacatac tcatcttccg ggcaacaatc gctcaccaca atatcaaagc gtttcaggtc
     5821 acccatgcgc gccggacgca ccttgccaaa tttactgtgg tcaacaacca gcacatgctt
     5881 ttgcgccatc gacatggccc agtgttttac cggcaactct tcaagattaa aacaggtagc
     5941 gcctttactg acatgcacgc ccgccgcaga ataaaaagcg atatccgggc aaaaattatt
     6001 cagcgtttgc tgaaaatcga tgggtttgaa aatggcgttg ctggcgtgaa attcaccacc
     6061 gcacagaaat gcgcggcaat ggggtttttc tttcagcgcc agaaaggtat ttagcgaata
     6121 acaaacggcg gtaaaaggga tttcattatc aatcgcttca ataatccacg gcgtggtggt
     6181 gccacagtca aaaaagaggg tctgatcggg ttctaccagt gtcgcagcca gttttgccgc
     6241 ccggcgtttt tcttccacca ggcgggattt ttgatcgctt aacaggtaat ggctggcgct
     6301 gcgcggttcc agaacaatat agccgccgag caaaacgacg ggcgcactgt ggttgttcag
     6361 atcgcgacga atcgtcatct ccgaaacccc aagcagggcg gcggcgtctt taagatgtaa
     6421 cttatcgctg cgttttaatt cttgcagcag ctgcccgata cgctcttcgc gacgtgtttc
     6481 cataatccct ctgaatagtt attgaagcga gccgctcaat actacacttt ttagcagaga
     6541 tcagtcacgc acccagcctt tgcggatcgg taatgcaaaa cagaagcgat accacgattg
     6601 cagacgttga tagagtttat gccccatcgc ttgccagata atctgggcac taaggcagcc
     6661 aatcatacct gccagcaatc caccgagcat atccagcggc cagtggacgc caagataaac
     6721 gcgcgaccag gcaatgacga cggccagcac cattaaaagt gagccggacc acaggcgatg
     6781 ccagcataaa aatgccagtg caaaggtgaa aatcaccgta ccgtgatcgc tcgggaatga
     6841 gtcatccgcc gcatgatgca ggaagttata gccgatattt tcgacaaacg gtcggtcgtg
     6901 cggaaaaaga tgtcccatcg tccaggacac caacaggctg accgccagcg cgatagcgat
     6961 ttttatcacc agttgccgtt gtgctgtaag cccccacaac caaagtaccg cggccagcag
     7021 cggcaccacg gtaatcaaat ctttagcaat aaaaatcgcc aacgagatca tccacggagc
     7081 cgagtctggc gtcgcgttaa taagagagaa tagagagaga ttcaaatttt ccagcataac
     7141 ttcccgacgc aaagtgatta aaagggagcc aatacaggca agtcgttgag aataaagtgc
     7201 aggttaaact gggtaaagcg gcatcgtctt atttccctca agcggcctgt ttacggtggg
     7261 tgattgtaac gggcataggt taaataaaac ttaaagaaag cgtagctata ctcgcaataa
     7321 tgtaagaatg tgcttaaccg tggtttcagc tacaaaattc gctttctcgt tagctgcgct
     7381 tttattaaac tctgcgcgat tattattggc gaagaaattg catgcaaaat aaattagctt
     7441 ccggtgccag gcttggacgt caggcgttac ttttccctct ctgtctggtg ctttacgaat
     7501 tttcaaccta tatcggcaac gatatgattc aacccggtat gttggccgtg gtggaacaat
     7561 atcaggcggg cattgattgg gttcctactt cgatgaccgc gtatctggcg ggcgggatgt
     7621 ttttacaatg gctcctgggg ccgttgtcgg atcgtattgg tcgccgtccg gtgatgctgg
     7681 cgggtgtggt gtggtgtggt ttattgtcac ctgtctggca atattactgg cgcaaaacat
     7741 tgaacaattc accctgttgc gcttcttgca gggcataagc ctctgtttca ttggcgctgt
     7801 gggatacgcc gcaattcagg aatccttcga agaggcggtt tgtatcaaga tcaccgcgct
     7861 gatggcgaac gtggcgctga ttgctccgct acttggtcca ctggtgggcg cggcgtggat
     7921 ccatgtgctg ccctgggaag ggatgtttgt cttgtttgcc gcattggcag cgatctcctt
     7981 tttcggtctg caactagcca tgcctgaaac cgccacgcgt ataggcgaga aactgtcgct
     8041 gaaagaactc ggtcgtgact ataagctggt gctgaagaac ggtcgctttg tggcgggggc
     8101 gctggcgctg ggattcgtta gcctgccgtt gctggcgtgg atcgctcagt cgccgattat
     8161 catcattacc ggcgagcagt tgagcagcta tgaatatggt ttgctgcaag tgcctatttt
     8221 cggggcgtta attgcgggta acttgctgtt agcgcgtctg acctcgcgcc gcaccgtacg
     8281 ttcgctgatt attatgggcg gctggccgat tatgattggt ctgttggtcg ctgctgcggc
     8341 aacggttatc tcatcgcacg cgtatttatg gatgaccgcc gggttaagta tttatgcttt
     8401 cggtattggt ctggcgaatg cgggactggt gcgattaacc ctgtttgcca gcgatatgag
     8461 taaaggtacg gtttctgcgg cgatgggaat gctgcaaatg ctgatcttta ccgtcggtat
     8521 tgaaatcagc aaacatgcct ggctgaacgg gggcaacgga ctgtttaatc tcttcaacct
     8581 tgtcaacgga attttgtggc tgtcgctgat ggttatcttt ttaaaagata aacagatggg
     8641 aaattctcac gaagggtaaa aaaatgcctg attgctttgt gcgatcaggc attctcgaat
     8701 taatggtgat ggtcgtcaat ctggtgttcg ataaccatcc cttcacctac gctggcaaga
     8761 tggcgaacat aaggatgcgg gtggtaagcc ggagctggcg caggagccac atagacggtt
     8821 tgcggcacag tcgcgacgct gaccgcttgt ggaacgctga ctgaatcagg aaccacaacc
     8881 actttgtatc cactcggcac atcgacggtg acactttgcg ccatcgccac accagtaaag
     8941 cccattaaaa gcgcgcccaa cagtagacaa tttttcataa tcatttcgcc tgaaataatg
     9001 cagcgggtta atttccgcga attatgcagc cttcattcag gtattgtgtt gccgggcgtg
     9061 ctgccgtttt tattggtcaa atggcgcttc gtgtttaaga actttatcga tcacatccag
     9121 tacgccttca cggttattgg agcctgcccg gtattttgcc gctgcgacga ccgcgttgcc
     9181 ggcattttcc attgcaaaac taaagcctgc ctgacgcagc atctcaatat cgttaccgcc
     9241 atcgccaaag accaccactt cgctgtcgtc tattccccat aatttctgca gttggcgaag
     9301 gccattggct ttatgtacgc cggggataat cagatcgatg ctgccgttgc cggtgtggac
     9361 cggcaccata atatcgccga tggcctcatg taatgttttt tgtacttgtg gaatcagttc
     9421 atcggaaaga ttcaggccaa acttaaagaa gatatcttct aagttgtcaa agttatcgac
     9481 gtattccaga cggtgataat acatttccgc caccattttc atggcatcgt catatttttt
     9541 gagcgtatag gcgctatttt ttccgcaggc aataatttcc acttccgggc gcgtcagcaa
     9601 atgttccacg acagtagtaa acgcatcctt cgacagctcg ccattaaaaa catctttgcc
     9661 ttcactcact acccagccgc cgttttcagc cacaaaggca atttcattag caatttcagg
     9721 gaagaaagag atcaactgat aatattgatt cccgctggcg accacaaagc gaatgccttg
     9781 tgctttcatt tgctgatact gagccataaa ccgctcacgg ttataggttt tttgatcgct
     9841 taagaaagta ccatccatgt ctaccgcaat taatttaatg ctcatcaact attctccatc
     9901 gccgtctgcg ttttggtatc gggtttggcg acggctttcg cgacaatagc cgcgagaata
     9961 accagcgcca gtacaaccag cattgcacta cgtaatccat aatgttcgcc gagatagccc
    10021 agcagcggcg gcccgacgag gaaagccaga taaccggtcg ttgctaccat actgacgcgg
    10081 gttggtgcat cggggccggt atcgctggcg gcagaaatgg tcagcgggaa gcccagcgag
    10141 gcacccagtc cccagagtac aacagacacc ccagcgaccc aggcgctatc gacaaaaata
    10201 atcagcccaa tacccaacgc ccccattagc gcactggccc gaaccacggc aacgcgactg
    10261 taacggtcga tgaaccaacc gccagtgaag cgtccaacgg tcatccccag ggtaaaaccg
    10321 gcataaatca gcgagccgga agtggggcta aaaccgtgac cgtcaaccat taataagggt
    10381 aaccagtcgt tggcagaacc ttcggcaaag gccatcgcca gcaccacaac accaatcagc
    10441 agcaactgga tatcgcgata aaaaggtacg cctttttcgc catgctgggt gccatcggca
    10501 gcatttttgc ccgtaccgtc agggattgcc tgaatggcga tatatatagg cgcgatgcct
    10561 accagcgccg ccaataaaat gtgcaccgtt gccggaacgc caaaggccgt cagtgccatc
    10621 ccgacaccag cgcctgccag cgtgcccagg ctataaaaac cgtgcatcat cggcaaaacc
    10681 gttttattca tttctcgctc aacggcggca ccttcaacgt ttatcgccac ttccgcagaa
    10741 ccaaaacttg ccccaaagac gccgagacca acggcaaaga gcaggggcga tgtcagccag
    10801 agtgccagac ttaatatcat catcccgacc aatgcgcagg acatggtgac caggatgaca
    10861 ttacgtgtcc caaagcgttt cactaaccac gccgagcaga gaataccgct catcgaaccg
    10921 atcgacagac caaagagaac accgcccatt tcagcgatcg agacagagag aatatcgcgg
    10981 atagcaggcg tacgggttgc ccaggacgcc attaacaggc ccggcaaaaa gaagaacata
    11041 aacagcgccc aggttcggcg tttcaatgca ttacgtgaag aatttacggt catagatcac
    11101 gtcaaaataa gaagagggaa gacaacatta gcaaggttgt gtacatttgt acacaattgc
    11161 agacagagga aatgacatgc gtcgcgctaa cgatccgcaa cggcgagaaa aaattatcca
    11221 ggccacgctg gaggcggtga aactttacgg aatacatgct gttacgcacc gcaaaattgc
    11281 tacccttgcc ggggtaccgt tgggatcgat gacctactat ttttcaggaa ttgatgagtt
    11341 gttgctggag gcgttcagca gttttactga gatcatgtcc cggcaatatc aggcattttt
    11401 tagcgatgtt agtgatgctc aaggcgcatg ccaggctatc accgatatga tctacagctc
    11461 acaggttgca acgccggata acatggagct gatgtaccag ctctacgcgc tggctagccg
    11521 aaaaccgcta ttaaaaacgg taatgcaaaa ctggatgcag cgcagtcagc aaacgctcga
    11581 acaatggttt gaacccggaa ccgcccgcgc gcttgatgcg tttattgagg ggatgacgct
    11641 gcattttgtc accgaccgta agccgctatc gcgcgaggag attttgagga tggttgagag
    11701 ggttgcaggg tagtagataa atttcaggca acaaaaaacc catcaacctt gaaccgaaat
    11761 ggcggggttg atgggctcca caaaatgggg acatcaaaga aaagcagtgg cactaattaa
    11821 gactgatgcc ctgcggaaaa gttctgcggt tgtgcaaaaa aatttcattt tcagggcaac
    11881 ttcagtttta tcctaatcct ggccatacca tgacgatgat tgtccctgcc agcgtcagca
    11941 ggacgttggc gattgcgtag gtgcccgcat agcccagcgc cgggatgtta ctgcgagctg
    12001 tatcactgat gatctccatt gccggcgcgc aggtgcgtgc gcccatcatt gcgccgaaca
    12061 acagtgcgcg gttcattcgc aatacataag caccgaacaa gaaacagata accacgggca
    12121 caagactgac aatcaatccg gcaatcaaca tctgaccgcc aatcgcgccc agaccgttat
    12181 taataccgct accggcgctc agaccaacgc ctgccataaa caccatcaag ccgaactctt
    12241 tcaccatgct taatgcccct tgcggaatgt aaccgaaggt cgggtggtta gcacgcataa
    12301 agcccagcat aattccggcg aataacaacc cggcagcgtt ccccatgccg aaactgaatg
    12361 tgctgaactg gaaggtgatc atcccgatca tcagcccaat aacaaagaag gcgcagaatg
    12421 ccagcaggtc agtgacctgg ctgtgaatcg agataaagcc gatgcgatcg gcgatggttt
    12481 ttacgcggcg ggcatcgccg ctgacttgta aaacgtcacc tttgttaagc acgacgttgt
    12541 catcaatcgg catctcaatc tggctacgaa tgacgcggtt aaggaagcaa ccgtgatcgg
    12601 tcaacttcag ttgtgcgaga cgtttaccta cagcgttatg gtttttaacg accacttctt
    12661 cagtgacgat acgcatgtcg agaaggtcac gatcgaaaac ttctttaccg ttacggaagc
    12721 tgggatcgag tcgggcatgg gcgtcgggat agcctaccaa cgctatttca tcgcccattt
    12781 gtagcacggc atcaccgtct ggatttgcca gaatcccgtt acgtcgaata cgttcaatgt
    12841 agcagccggt ttgtcgataa atacccagtt cacgcagatt tttgccgtcg gtccaggcca
    12901 ccagctccgg gccgacgcga taggcgcgga tcaccggtaa ataaacctta cggttggcat
    12961 cagtgtccag gccacgttcg cgggcgattt gctgggcgct ggtctgtaag tcctgatgct
    13021 gcaatttcgg caagtaacgc gcaccaacaa tcaaactcac cagaccgatt aaataggtta
    13081 aggcataccc gaggctcaga ttatccagtg ccagtgagag ctgcctgctt tccataccgg
    13141 aatgacgcag tgtatcgcca gcaccgacca gaaccggtgt cgacgtcata gagcctgcta
    13201 acataccggc cgtcaggcca atatcccagc caaacagctt acctaaccct aaggcgatca
    13261 ccagcgcact gccaaccatc accagtgcta acattaggta atttttccca tcgcgaaaaa
    13321 aaatggaaaa aaagttcggt ccggcttcga ccccgacgca gaaaataaac agcataaagc
    13381 caagattaag cgcatcggtg ttaatgctga aatgttgttg gcctaataac agcgatacga
    13441 ctaaaacgcc aatggaatta cccagttgga tcgaaccaag tcgtaacttt ccgagacata
    13501 gcccaagcgc gaggaccaca aataataaca gaatgtaatt cccatttaac aattcggcga
    13561 cgtttatatt cacggaggct aacttcttgt ttactagtaa gctgttgaaa gaaatggtaa
    13621 tttacgataa tgttttttac cagaattcag ggcgcagatt cattcagcgc acctaaacga
    13681 tagtaaagta acaatatatt ttactagtgt aatcacatta ggtatcaacg gctatatgaa
    13741 ttgcgttggc ctatattagc atggaatgcg aagcggcttt atcttactga acgccacact
    13801 ggcgaaaaat gtgttcgata gacgcagtgt caggaggaac gagtgaaaca taaacaacgt
    13861 tgggcggggg caatctgctg ttttgtcctc ttcattgtgg tgtgcctttt tctggcgacg
    13921 cacatgaaag gcgcttttcg ggctgccggg catcctgaaa tcggcttgct gtttttcatt
    13981 cttcctggag cagttgccag tttcttttca cagcgtagag aagtcctgaa acctctattt
    14041 ggcgcaatgc tggcggcacc ctgttcaatg cttattatgc ggctgttttt ttcaccgacc
    14101 cgctcattct ggcaagagct ggcatggtta ctaagcgcgg tgttctggtg tgcgctgggg
    14161 gcactgtgtt tcttatttat cagtagcttg tttaaaccac agcacagaaa aaatcagtaa
    14221 agccctcaac gcgagggctt gtcagacgat caggcgtcca gattctcttt cacccatgca
    14281 gcaaaatcgg tatagccgcc gatatgttgc tgatcgacaa aaatctgcgg cacggtttct
    14341 acgggtttac ctgccttttg ttgtagatct tctttagtga tcccttccgc acgaatatct
    14401 acatactgat actgaaaatc atcgcgttca ttgctcaatt tctcagccag atcttttgca
    14461 cgcacacagt aagggcaacc cggacgacca aaaataacgg tttgcattat ttctctcctc
    14521 atagatttat gcctgtaatg atcacgctaa aatgtattcg ctgaaagtag gtttaacctg
    14581 ttgcattaat tgctaaaagc tataactgtt aaacacaata cagtgaaaag ttttagactg
    14641 aaggttcact ttgcagaggg aagcgtatgc gcgcgatcgg taaattgcct aaaagcgtgt
    14701 tgatactgga atttatcgga atgatgttgc tggcggtggc gctgctgtcg gtaagcgact
    14761 ccctgtcgct gcctgagcca ttttctcggc cagaagtgca gattctgatg atttttctcg
    14821 gtgttttgct catgcttccc gctgcggtgg tggttattct tcaggtggca aaacgtcttg
    14881 ccccacagct gatgaaccgt ccaccgcagt attcacgttc agaaagagaa aaagataatg
    14941 acgccaacca ttgaacttac ttgtggccat cgctccattc gccatttcac tgatgaaccc
    15001 atttccgagg cgcagcgtga ggcgattatt aacagcgccc gtgcgacgtc cagttccagt
    15061 tttttgcagt gcagtagcat tattcgcatt accgacaaag cgttacgtga agaactggtg
    15121 acgctgaccg gcgggcaaaa acacgtagcg caagcggcgg agttctgggt gttctgtgcc
    15181 gactttaacc gccatttaca gatctgtccg gatgctcagc ttggcctggc ggaacaattg
    15241 ctgctcggtg tcgttgatac ggcaatgatg gcgcagaatg cattaaccgc agcggaatcg
    15301 ctgggattgg gcggggtata tatcggcggc ctgcgcaata atattgaagc ggtgacgaaa
    15361 ctgctgaaat taccgcagca tgttctgccg ctgtttgggc tgtgccttgg ctggcctgcg
    15421 gataatccgg atcttaagcc gcgtttaccg tcctccattt tggtgcatga aaacagctat
    15481 caaccgctgg ataaagacgc actggcgcag tatgacgaac aactggctga atattacctc
    15541 acccgtggca gcaataatcg ccgggatacc tggagcgatc atatccgccg aacaatcatt
    15601 aaagaaagcc gcccatttat tctggattat ttgcacaaac agggttgggc gacgcgctaa
    15661 aaccgccacg ccgatgtatg atacgcgggc ttttgaccag gtctgacaga gaggtgcagg
    15721 gtgaaaattg ccatattgtc ccgggatgga acgctctatt cgtgtaagcg gctgcgtgaa
    15781 gccgctatac agcgcggtca cctggttgaa attcttgatc cgctttcttg ctacatgaac
    15841 ataaatcctg cggcgtcttc tattcactac aaaggccgca agttacccca ttttgacgca
    15901 gtgatcccgc gtattggcac cgccattacc ttttatggga cggcggcact gcgccagttc
    15961 gagatgctgg ggagctatcc gctcaatgag tcggtcgcca ttgcccgggc gcgtgacaaa
    16021 ttgcgttcca tgcaactgct ggcgcgtcag ggcatcgacc tgcctgtcac gggcattgcg
    16081 cattcgccgg atgataccag cgatttaatc gacatggtcg gtggtgcgcc gctggtggtc
    16141 aagttggttg aaggcacgca gggaattggc gtcgtgctgg cggagacgcg tcaggcggcg
    16201 gaaagcgtga ttgacgcttt ccgcggtctg aacgcgcata ttctggtgca ggaatatatc
    16261 aaagaggcgc aagggtgcga tatccgctgt ctggttgttg gcgatgaagt ggtcgctgcg
    16321 attgaacggc gggcgaaaga gggcgatttt cgttccaatt tgcatcgtgg cggcgcggca
    16381 agcgtcgcca gtatcacacc acaggagcgt gaaatcgcga taaaagccgc gcgaacgatg
    16441 gcgctggacg ttgctggtgt ggatattctg cgtgctaatc gcgggccgtt ggtgatggaa
    16501 gtgaatgcgt cgccggggct ggaaggaata gaaaaaacca ccggtatcga catcgcgggt
    16561 aaaatgatcc gctggatcga acgccacgct acgacagaat attgcctgaa aacgggtggt
    16621 tagtcgcaat cacattactg atcatggttt tgcctgctct ttttgcgtaa gctgtgccgg
    16681 tctttttatc gaaagaggtt gtacaaaatt atgacatcgc tggtcgttcc tggtctggat
    16741 acgctgcgtc aatggctcga tgacctgggg atgagttttt ttgaatgtga taactgtcag
    16801 gctctgcatc tgccccatat gcagaatttc gacggtgtct ttgatgccaa aatcgatctg
    16861 atcgataaca cgatcctgtt ttctgccatg gcggaagtcc gaccttcagc cgtattgccg
    16921 ctggcggcgg atttatctgc catcaatgcc agttcgctga ccgtgaaagc atttcttgat
    16981 atgcaggatg ataatctgcc aaagctggtg gtttgccagt ctttatccgt tatgcagggc
    17041 gtaacctatg agcagtttgc atggttcgtg cgtcagagcg aagagcagat ttcgatggtc
    17101 attcttgaag ctaatgccca tcaactgctg ttaccgactg acgatgaagg gcaaaacaac
    17161 gttaccgaaa actatttcct ccactgataa ctcctttcga gcacgcagtc gctggtgcag
    17221 tggctgcgcg ctgcaaaatt atctgctgtt tttaaccttt tcttaaagat tatttcactt
    17281 ctcttgtgtc gatttggctt tatcacatag agcaaatacg cataaaaatt tgttaaatac
    17341 cgttttttaa tccgagctat agtctcaaac cctggctaaa gttattcttg cgatgctttt
    17401 atatagcgag cagtgctggc cgggagagag ttctcttttc ttacaccgcg ccgataaaaa
    17461 atatgcacgt ttattgcata tctttcagtg tgacaacttt tgttcgtttg ttaacgaact
    17521 ttcagaagga aagagatatg accgccttaa ataaaaaatg gctttcgggt ctggttgcgg
    17581 gtgctctgat ggccgtctct gtcggcacgc tcgcggctga acaaaaaaca ctccacattt
    17641 ataactggtc tgattatatc gcccccgaca cggtggctaa ctttgaaaaa gaaaccggta
    17701 ttaaagtcgt ctacgatgtt ttcgactcta acgaagtact ggaaggcaaa ttaatggccg
    17761 ggagcaccgg ctttgacctg gtggtgccgt cagcaagctt tcttgaacgc cagctgacag
    17821 cgggagtttt ccagccgctg gataaaagca aattgccgga gtggaagaat ctcgacccgg
    17881 aactgctgaa gttggtcgcc aaacacgatc ccgacaataa atttgctatg ccctatatgt
    17941 gggcgacgac cgggattggc tataacgtcg ataaagtcaa agcggtgctg ggcgaaaacg
    18001 cgcccgtcga tagctgggac ttgatcctca agcctgaaaa tctggaaaaa ctgaaaagct
    18061 gcggtgtatc tttcctcgat gcgccagaag aagtttttgc taccgtgctg aattatctcg
    18121 gcaaagatcc caacagcact aaagcggatg attacaccgg accggctacc gatctgctgt
    18181 taaagctgcg cccgaacatt cgttatttcc attcatctca atatattaac gacctggcaa
    18241 acggcgatat ttgcgtcgct atcggctggg caggtgatgt ctggcaggcg tcaaaccgcg
    18301 cgaaggaagc gaagaatggc gtgaatgtct cgttctcgat tccaaaagaa ggggcgatgg
    18361 cgttctttga tgtattcgcc atgcctgcgg atgccaaaaa taaagacgaa gcctatcagt
    18421 tcctgaatta cctgctgcgt cctgatgtgg tggcacatat ctctgaccat gtgttctatg
    18481 ctaacgccaa taaagcagcc acgccgctgg tgagtgcgga agtccgtgat aacccgggta
    18541 tttatccgcc tgcggatgtc cgcgcgaagt tgttcactct gaaagtacag gctccgaaaa
    18601 tcgaccgtgt gcgcacccgc gcgtggacca aagtgaagag cggaaaataa tccgcagcct
    18661 tagatgccgg gcgggagcgc cactccctcc ggcaattcgc accaacatgg tgcgcttgca
    18721 cacatttaat gccggagagc agccgtgaat gacgctatcc ctcgccccca ggcgaaaacc
    18781 cgtaaggcgc tgaccccgct attagaaatc cgcaatctga ccaaatccta cgatggtcag
    18841 catgcggttg atgatgtcag cctgaccatc tacaaaggtg aaatctttgc gctgctgggc
    18901 gcatccggct gtggcaagtc cacgctgctg cgtatgctgg caggtttcga acaaccttca
    18961 gccgggcaga ttatgcttga tggcgtcgat ttgtcacagg tgccgcctta cctgcgcccc
    19021 atcaatatga tgtttcagtc ttacgcgctg tttccgcata tgaccgtgga acagaacatc
    19081 gcttttggcc tgaaacagga caaactaccg aaagcggaaa ttgccagccg ggtcaatgag
    19141 atgctcggcc ttgtgcatat gcaggagttc gccaaacgca aaccgcatca gctttccggt
    19201 ggtcagcgac agcgtgtggc actggcccga agccttgcca agcgcccgaa actattactg
    19261 ctcgacgagc cgatgggcgc gctggataaa aagctgcgtg accggatgca gcttgaagtg
    19321 gtggatattc tggaacgcgt cggtgtgact tgtgtgatgg tcacccacga tcaggaagag
    19381 gcgatgacca tggcggggcg tatcgccatt atgaatcgtg ggaaatttgt ccagattggc
    19441 gaaccggaag agatctacga gcatccgact acccgctata gcgccgaatt tattggctcg
    19501 gttaatgtct ttgaaggtgt actcaaagag cgtcaggaag atggtctggt gctttattcg
    19561 ccggggctgg tgcatccact gaaagtcgat gcggatgctt cagtggtcga taacgtgccg
    19621 gtacacgtag cgctgcgtcc ggaaaaaatc atgctttgcg aagagccgcc cgccaatggt
    19681 tgtaacttcg cggtggggga ggtgatacac attgcctatc tcggcgatct ttcggtgtat
    19741 cacgttcgtc tgaaaagtgg gcagatgatt agcgcccagc tacaaaacgc ccatcgtcat
    19801 cgtaaagggt taccgacctg gggcgacgaa gtgcgtttgt gctgggaagt ggacagctgt
    19861 gtggtgctga cggtttaagg agcaaagatg agtacacttg aacctgctgc ccagtcgaaa
    19921 ccgccgggcg gatttaagct gtggttgtcg cagttgcaaa tgaagcatgg gcgcaaactg
    19981 gtcattgcgt tgccgtatat ctggttgatc ttgctgtttc tgctgccatt tctgattgtc
    20041 tttaaaataa gcctggcgga gatggcgcgc gctattccac cttataccga actgatggag
    20101 tgggctgacg ggcaactgtc catcaccctt aatttcggta atttcctgca actgaccggc
    20161 gatccgctct atttcgatgc ttatctccag tcgttacagg tggcggcgat ttcgacattt
    20221 tgctgtttac tgatcggcta tccgctggcg tgggcggtgg cgcacagtaa gccttcgacc
    20281 cgtaatatct tattactact ggtgatcctg ccgtcgtgga cctcgtttct gattcgtgtc
    20341 tacgcctgga tgggaatatt aaaaaacaac ggtgtgctga ataattttct gctgtggctg
    20401 ggggttatcg atcaaccgct gaccattctt cataccaatc tggcagttta tattggcatt
    20461 gtttacgcct acgtgccgtt tatggtgctg ccgatttata ccgcgttgat tcgtattgat
    20521 tattcgctgg tggaagcagc gctggatctc ggtgcacgac cgctgaaaac gttctttact
    20581 gtgatcgtgc cgctgactaa aggtgggatt attgccggat cgatgctggt gtttatcccg
    20641 gctgtgggcg agtttgtgat cccggaactg ctcggtggcc cggacagcat catgatcggg
    20701 cgcgtgctat ggcaagagtt tttcaataac cgcgactggc cggtggcctc ggcggtagcg
    20761 atcatcatgt tgctgctgct aattgtgccg ataatgtggt ttcacaaaca ccagcaaaaa
    20821 agcgtgggag aacacggatg aataatttac cggtagttcg ttcgccctgg cggattgtga
    20881 ttttgctgtt gggcttcacc tttctctacg cgccaatgct gatgctggtt atctattcgt
    20941 ttaacagctc gaagctggtg acggtgtggg ccggctggtc aacgcgctgg tatggcgaat
    21001 tattgcgcga tgacgcgatg atgagtgcgg ttggtttaag tctgacaatt gcggcctgtg
    21061 cggcgacggc ggcggcgatc ctcggaacta ttgcggcggt ggtgctggtg cgctttggca
    21121 ggtttcgcgg atcaaatggc tttgccttta tgatcaccgc gccgctggta atgccagatg
    21181 tcatcacggg cttgtcgctg ttgttattat tcgtcgcgct tgctcatgcc attggctggc
    21241 ctgcggaccg cggtatgctt accatctggc tggcgcatgt cactttctgt acggcttatg
    21301 tggcagtcgt tatttcgtcg cgtcttcggg aactggatcg ctcgatagaa gaagcagcga
    21361 tggatctcgg tgcgacgccg ctgaaagtgt ttttcgtcat tacgctaccg atgatcatgc
    21421 ccgcggtcat ttctggctgg ttactggctt ttactttgtc gcttgatgat ctggtgatcg
    21481 ccagctttgt atccgggccg ggagccacca cgttaccgat gctggtcttt tccagcgtgc
    21541 ggatgggggt gaatccggaa atcaacgccc tagcaacatt gatcctcggc gcggtcggaa
    21601 ttgtcggatt tatcgcctgg tatctgatgg ctcgcgcaga aaaacagcgg atacgcgaca
    21661 tccagtgtgc aagacgtggc tgaagacact aaaatttgcc aacctggcta cataatgccg
    21721 cgtatgtcgc ggcattgttt tcatggaaga cgaaacgttg ggatttttta agaaaacatc
    21781 ttcatctcat gctcgcctga atgtgcctgc gcttgtgcag gtggcggcgc tcgccattat
    21841 tatgatccgt ggcctcgacg tgctgatgat tttcaatacg ctgggcgtgc gcggtattgg
    21901 cgagttcatt catcgcagcg tacaaacctg gagtttaacg ctggtctttt taagcagtct
    21961 ggtgctggtt ttcattgaga tctggtgtgc gttttcactg gtgaaagggc gtcgctgggc
    22021 gcgctggctg tatctgctga cacaaatcac tgccgcaagt tacttgtggg cggcttcgct
    22081 ggggtacggt tatccggagc tgttcagcat tcccggtgaa tcaaaacgtg aaatcttcca
    22141 tagcctgatg ctgcagaagc tgccggatat gctcatcctg atgctgctgt tcgttccctc
    22201 gaccagtcgg cggttcttcc agttgcaata atgtgtataa tcgtcgcccc tgatgatgtg
    22261 aaggtcaatg tatgcagtgc gcactttacg acgcgggtcg ctgtcgttcc tgtcagtgga
    22321 taacgcagcc gattccagag caactctccg ctaaaaccgc cgatcttaaa aatctgctcg
    22381 ccgactttcc ggttgaggaa tggtgcgcgc cggtgtcagg cccggaacaa gggtttcgta
    22441 ataaagccaa aatggtggtg agtggtagcg ttgaaaaacc actgctcggt atgctgcatc
    22501 gcgacggcac accggaagac ctttgtgact gcccgcttta tcctgcctca tttgcgcccg
    22561 tttttgcggc gctaaaaccg tttatcgccc gagcggggtt aacgccctac aacgtggcgc
    22621 gtaagcgtgg cgaactgaaa tacattctgc tgactgaaag ccagagcgat ggcggcatga
    22681 tgctgcgctt tgtactgcgt tctgaaacca aactagcaca actgcgtaag gcattgccgt
    22741 ggttacaggc acaattgccg cagctaaaag ttatcaccgt caatattcag ccggtacata
    22801 tggcgattat ggaaggggag acggagatct acctgaccga acaacaggcg ctggtagagc
    22861 gttttaacga tgtgccgctg tggatccgtc cgcaaagttt cttccagact aatccggcgg
    22921 tcgccagcca gctgtacgct accgcgcgcg actgggtacg acagctgccg gttaaacata
    22981 tgtgggatct gttctgcggt gtggggggct ttggtttaca ctgcgcgacg cctgacatgc
    23041 agttaaccgg gatcgaaatt gcgctagagg ccattgcctg tgcaaagcag tcagccgctg
    23101 aactgggctt aacgcgtctg caatttcagg cgctggactc cactcagttt gccaccgctc
    23161 agggggaggt gccggagctg gtgctggtta acccgccgcg ccgcggcatt ggtaaaccgc
    23221 tgtgtgatta tctctcaacg atggcaccgc gttttattat ctactccagc tgtaacgccc
    23281 aaaccatggc gaaagatatc cgcgaactgc caggttaccg catcgaacgg gtacagcttt
    23341 ttgatatgtt cccgcacacc gcgcactatg aagtgctgac gctgctggta aaaatgtaaa
    23401 tacatcattc aaatcaatat cacctcggca tacatccatc caaaataata atcgtgatcg
    23461 atcgattaat aataaccgat cgatcacggt tatatattgc tctatttttg caaatcaaat
    23521 cacaaataat caaataagat ctttaattga actgttttga ttactacagt gcacttaccg
    23581 aatacataca gaggcattgc ttagcgaaac acaaaatttc ctgagacatt attaacgtca
    23641 gcagaaggtt catgcattga caacggaaat gtacgcgtat ggagggcgtt ccgacgatgt
    23701 ttgccaaatt catagatgtt attcaaacgt ttttaactga accggcaatt ttaattggtt
    23761 tgctggtggg tattggttat gcccttgata agaaatcgcc aataaaaatt attaccggca
    23821 tggtcagcgc catggtggga ttaatgatgg tgttatttgg tggtttccag ttttccgcaa
    23881 catttaaacc ggtagcagaa gccgtaagta aagcctatgg tgttcacgga tatttaatgg
    23941 attcctacgc aatgaaagcg gcaacgcaaa ttgcattggg ggataatttt ggttatgtcg
    24001 ggtatgtatt tgtactggcg ttttttacca acctgctgct ggtattattt ggtcggtata
    24061 ctggtgccaa agggatattt ttgacaggga ataccggtgt ttcacattct caggcggtat
    24121 tatggttgat tgtcttttgg ctgggtttta gctggactac atccattatt attgccggaa
    24181 tattaacggg tgtattctgg gcgttctcca ccacgctcat tgttaagccc attgccaaag
    24241 ttaccaaaga tgccggtttt accattgctc ataaccagat gttgggattg tggtttttct
    24301 ccaaattcgc ccataaattt ggtgaccctg aaaagcacga tgctgaaaac ctgaaacttc
    24361 ccgggtggct ggcgattttt aaccataacg taacggctat cgccattgtg atgacgctgt
    24421 ttgtcggtgg tttcttgcta tcaacgggta tcgataatgt ccagttaatg gcgaaaggca
    24481 agccctggta catctatatc atcaaccttg gcttacagtt ctccatgtat atggtcattc
    24541 tgctgcaggg tgtgcgcatg atggtgggcg aaattaacgg ttcgtttaaa ggctggcaag
    24601 atcgctttat tcctaacgct attcctgctg tcgatgttgc agcgctgtta cctttttcac
    24661 ccaatgccgc aacgttaggc ttcgtcttct gtacttttgg caccattttt tcgatgggga
    24721 tcttattact ggttcacagc ccaattatgg tattgcctgg ctttgtacct ctgtttttct
    24781 ctggcggtcc aattggcgta ttagcaaacc gcatgggcgg ataccgttcc gtaattatct
    24841 gtacgttctt gctgggtatt attcagacct tcggtacggt gtgggctatt ccgttaaccg
    24901 ggcttgctga aaatggcgtc ggctggacag gaatatttga ctgggcaacc gtatggcctg
    24961 ctatttgtga agttctgaaa tttatcgctg caacattcca tcttggtcct tacgcgggtt
    25021 aattaaatat taagggttta aaaatgaaag atacaattaa tattctgttt gtctgtggtt
    25081 acggtgtagg cagtagcgtt atgttacaaa cggtcgttaa aaaagcgctg accaaatatg
    25141 atttcaaagt tgaaatggaa cataccgccg caggtgaagt cggcggattt accgactggg
    25201 cggatatttt tgctatttcc aaaaaattac tcgatgtggt tagtcttgac ccgaaacaag
    25261 ggcaatattt gattccgata gagaatatta tggacggtga aactattggt cagcaaattt
    25321 atgaagtggt tcagcaacat tttccgcatt taattaatca ataaggtagg gcgtaatgag
    25381 agcgattatt ctgctgttcg atagtctgaa taaacgttat ctgccacctt atggcgatgc
    25441 gttaaccaaa gcgcctaatt tccaacgtct ggcggctcat gccgccacct ttgaaaacag
    25501 ttacgtcggc agtatgccgt gtatgcctgc ccggcgggag ttgcataccg ggcgatgtaa
    25561 cttcctgcat cgggagtggg ggccgttaga accgtttgac gattccatgc cggagctgct
    25621 taaaaaggcg gggatctata cccacctgat aagcgatcat ttgcattact gggaagatgg
    25681 cggcgggaat tatcataatc gctacagttc gtgggagatt gtgcgcggac aggaaggcga
    25741 tcactggcat gcaagcgttg cgcaaccgcc tattcccgag gtgctacggg tgccgcaaaa
    25801 gcagaccggt ggcggtgttt ctggtctgtg gcgccatgac tgggcaaacc gggaatatat
    25861 tcagcaggaa gcggattttc cacaaactaa agtgtttgat gcaggctgtg cctttatcca
    25921 caaaaatcac gcggaagata actggttatt gcagatcgaa acgtttgatc cgcacgagcc
    25981 gtttcacaca acggaagagt atctttcctt gtatgaagat aactgggatg gaccgcatta
    26041 tgactggccg cgtggccggg tgcaggaaag cgacgaggcc gtggagcaca tccgttgccg
    26101 gtatcgttcg ctggtgtcga tgtgcgatcg caacctgggg cgcattctcg acctgatgga
    26161 tgagcacgat ctgtggcgag ataccatgct gattgtcggc actgaccacg gctttttact
    26221 cggtgagcat ggctggtggg cgaaaaacca gatgccttat tacaacgaag ttgccaataa
    26281 tccgttgttt atctggaacc cgcgcagtgg tgtaaaagga gagcggcgac aggcactggt
    26341 acaaatgatc gactgggcgc caacgttgta tgactttttt caacagccag tgccgcccga
    26401 tgtgcagggg caaccgctgg cgaaaacggt cagtcacgat gaaccagtac gcagctcggc
    26461 gatgtttggt gttttcagtg gtcatgctaa cgtaactgat gggcgttatg tgtatatgcg
    26521 tgcagcgctg ccggggcgtg aggatgatat tgccaactac acgttgatgt cctgcaaaat
    26581 gaacagccgc tatccggtgg atgagatgcg ggctttatcg ctggccccac cgtttcgttt
    26641 taccaaaggg ttacaggtat tacgcatccc ggcacaggaa aaatataagg ggttgaatca
    26701 gtttggtcat ttgctgtttg atctgcaaaa cgatccgcag cagctacatc cgattcatga
    26761 tgatgtgatc gagtcccgga tgatcgcgtt gctgattcag ttgatgaaag ataatgatgc
    26821 gccgccagag cagtttcagc gcctgggatt agcgtagaga agtgattttg ccggagtggg
    26881 tgttcgccca ctccggcaaa tgaataatta ttgcgggaac cactgatcgc tgatttgttg
    26941 atatgtaccg tcagctttaa ttgctgccag cgcgttattc agtttttcca gcagagcttt
    27001 gttatccgga cgtacagcga tacccagacc ggtgccaaag tattgcggat cggtcacttt
    27061 ttcagtagca acgcccagtt gcggattggt tttcagccat tcattaacca ccgcagtgtc
    27121 accaaatacg ccatcaatac ggctattttt cagatcgata aaggcatttt gataactgtc
    27181 ataggagacc gttttcactt ccgggtgctg atcctgaata tatttctggt gcgtagtacc
    27241 gttttccatc ccaatgcgtt tgcctttcag gtcggcaaac gttttgtagg tatctttttt
    27301 ggcaatcacg acggctgagt tttcgtaata gggcgtggtg aacgatacct gcttgctacg
    27361 atctggtgtg atatccatac cggagattac ggcgtcatat ttcctgaatt tcagtgacgg
    27421 gatcaggctg tcgaacgcgt gattggtaaa agtacattct gcctgcatct gtttgcataa
    27481 cgcttttgct aggtcgatat caaagccgac gatctggtta ttggcatcca tcgactcgaa
    27541 cggcggatag gtcgctgata cgccaaaatt gattttctct gcggcagaag cgccgaacgc
    27601 acaggatgcc agtaatgcgg caagaactaa ctttttcatg atggaactcc cgtctgtcaa
    27661 tcttatgatt attggccgtg tctgcggcat gggataacaa tgccatcaag tgaatttata
    27721 tgcaataaat atgattaaat aatttgcatg aaataaaaaa agacggacaa cttagtgagt
    27781 tgtccgtctt tattataaga atttatgcac tatgtaggcc ggataaggcg tccacgccgc
    27841 atccggcaca ggcaccgtgc tgatgtctga tgcgacgctg gcgcgtctta tcagacctac
    27901 aaaacccccg gcgaatgtac gcagccacat taattccttc gctcaaacgc cagcgctttg
    27961 cgctcgatca gacgcatcat cagcgtcagc aggccgttaa cgaccagata aataatccct
    28021 gccgcgccga acaccatgac atcgtaggtg cgtccgtaca acaactggct gtatcccatc
    28081 acttccatca gcgtaatggt gtaagccaga gaggtacttt tgaacaccag caccacttcg
    28141 ttggaataag aagagagcga gcgtttaaag gcatacggca gcaggatcgc cagcgtatct
    28201 tttttgctca ttcccagggc gctacaggac tgccactgac cttccgggat cgcacgaatt
    28261 gcaccgtaaa acagctgcgt ggtatacgcc gcactgttca gcgacaacgc aatcagcgca
    28321 cataaccacg gttctgacaa caaatgccac agtgccggat actcctgcaa cgtcgggaac
    28381 tgacccggcc cgtaataaat caggaagatc tgcaccagca gcggtgtacc ggtaaacagc
    28441 gtgatataac cccgcaccag ccacaccagc accggcgttt tcagcgtcag gatgatggta
    28501 aaaatcaacg ccagaatcag tgccacaatc agcgaggcaa cggttagcgt caggctggtg
    28561 tgcagccctt tcatcagttc gggtaaatac tcaaacatta gctgggcctc cgctcaaaac
    28621 gtgtcgcgcg caggtcaatg cgtttgagaa tgtactgact gagcagggtg atcaccaggt
    28681 aaatcgccgc cgccacaatg taccaggtaa atggttcctg ggtacgagta gcgatgcttt
    28741 tggtttgcag cattaaatca ttcacactaa tcaaactgac cagcgcggta tctttcagca
    28801 gcaccagcca ctggttaccg aggccaggca gcgcatgacg ccacatctgc ggcatcacca
    28861 gacggaaaaa gatagccgat ttcgacagcc ccagcgcctg gccggattcc cactgaccca
    28921 tcggcaccgc tttcaacgcg ccgcgcagcg tttgcgaggc ataggcggca tacagcagtg
    28981 acagagcgat gacaccacaa aggaacgggc taacgtcgaa gttttcaatc tgcatctgca
    29041 ccgggatctc tacaatccca agattgatag tgaagccatc cgaaagtgtc agcagcagct
    29101 gcgaggagcc aaaatagata aacagcacca ccagaatttc tggcaggcca cgcagaatgg
    29161 ttaccagcgc tgaacctgcc cacgcgacag gacgccattt cgcagactcc cataccgcaa
    29221 agaacatcgc cagcgccagc ccgacaatca atgcacaaac ggcaaggccg acggtcatcc
    29281 cggcggcgct tgctaaagga aaaaattcat tcatcaggaa ttacttctgg aaccatttgt
    29341 tgtagatggt ttcgtaagtg ccatctttct tcactttttc cagcgcagtg ttgagtttct
    29401 gctgcagctc agtgttgccc tgacgtaccg cgatgccgag gccagtaccg aagtaatctt
    29461 tatcggtcac tttatcgccc actgccgcca gtttcgggtt atctttcagc cattcagtta
    29521 ccactgcggt gtcaccaaat acgctgtcga tacgaccgtt ttgcagatcc agttttgcgt
    29581 tctggtagct gtcatacggc acggtggtga tttccgggtg cttatccata atgaatttct
    29641 ggtgagtcgt accattctgt acgccgactt ttttgccttt cagctgatca acgctggtgt
    29701 atttaccctg ctgacccaca aacagggcag agttgtcata gtacggcgtg gtaaacagta
    29761 cctgcttttc acgttccgcg gtgatatcca tcccggccat caccgcttcc acgcgacgga
    29821 atttcaggct tgggatcagg ctgtcaaatg cctggttaga gaaagtacag gtcgcatcaa
    29881 tctctttaca cagcgcctgt gccaggtcga cgtcaaaacc aacgatctgg ttgtttgcat
    29941 caatcgattc aaacggagga taggaggctt cggtagcaaa acgaatggtt tcggcagctg
    30001 tggcggaaag actaaaacct gcaattaacg cggcaatcag aacttttttc attgttgtta
    30061 tcccgaatct tagtgagaga gatagttttt aaatgcttcg gtttgcggct cggtaaagca
    30121 gctcgcgtcg ccttgttcta cgatatgacc gttttccata tacaccactc ggctggcggt
    30181 tttacgcgcc acttcaactt cgtgggtgac gatcacctgg gtaatattcg tttctgccag
    30241 ctcacgaatg atgctgacga tttgtgccgt aatttccggg tccagtgcgg cggtcggttc
    30301 atcaaacagc agtacctgcg gttccatcat taacgcacgg gcaatagcaa cacgctgctg
    30361 ctgaccacca gaaagatgca gcgggtaacg atcgctataa ggtttgagac gcagacgttc
    30421 cagcagtttt tctgcacggg ccagcgcctg atctttactc aaccccagta cacggcaggg
    30481 cgcttcaatc aggttttgct gcacggtcag atgcggccac aggttgtatt gctgaaacac
    30541 catgccaacg ttacgacgca aatcgcgaat cgctttgtct gagggtgttt tggtgaaatc
    30601 gaaatggttg cctgcaatgt tgagcgtacc ggagcgcggc atctcaagca gattgagtac
    30661 acgcagcagc gagcttttac ccgcgccgct ggggccaagt aacaccagcg tttcgccctg
    30721 tgggcaatcc agcgtgatat cgaacagcgc ctgatgcgcg ccgtagaagc aattaatgcc
    30781 gtttaattga atactcattg acactcgtat actggcagtc tgatagctat tgaggtcgaa
    30841 gatagtacct ttgacagaat aattatgcaa tatttctgct ttaaaagtta aaagcaaagc
    30901 gcattattca ataaacatag cacaaaataa cgggggcggt ggtcggcgag cataaatgtc
    30961 ggcattcctc acgaaatgcc ggacaattta cggggtttat tggttgatca aggcgttagc
    31021 gattctcgat ggactgacgg agcgtacccg ccgtggcatg aacgctaccg cctaagtagc
    31081 gcacatcgtc gatcacccaa cactggcctt cctgaatcat taacacttca tcctgccagc
    31141 cctggtcacc ctgcttgaga tccacgcgca atggaatgtt acgggcatca cgattaggga
    31201 tagtcgatgc actggcaacg tgggcgctgt ctggcaaggt ggttcgactg gagaacggat
    31261 cactgcttaa caattcacga tgactattat cgcggctggc atcgctaagt agggttgcca
    31321 gtttgtcact caaatagggg cgcagggcgg taatgtcatt gctgcgatgt tgaatgtggt
    31381 agtcatagaa ttgttgggcc acgctgtcag gccccccctg gacacaggaa ccggtgcggg
    31441 gaccattatc tttataagct ggcgtgacgg tggtacaggc actcagcagc agtgcacatg
    31501 gaataagtag agatagtgaa cggtagcgca taatcatttc cttattagct tgctgtctgc
    31561 gtcacactac gaccaaacgc tatcagatta tttaatacat tgtttttaat acaattctga
    31621 aacaatctga acacctgatt gggtttgagt ccattgctgg cgacccactc aaggttgctc
    31681 gtagctgact tacttaaatt acccaaacaa tagacagtgt aaaccatttg tgaggttgag
    31741 cgtgttatat ggctgaaatg atcatttaag gtaaatatga tagttctgaa atggtggtat
    31801 aaatcctcct gtagaggtta agagcatctg caagaagcca gtttcacatt tataacgcgt
    31861 gataaatgaa cgcggttatt gtctaatcat tacaaatttt tcacatgcga taagagatcc
    31921 tgctattaag atgattgcca aaattacgtt gtatttttga aaatgagcga aatttgatag
    31981 tcccag
//
LOCUS       DAZKIP010000045        31757 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZKIP010000045 DAZKIP010000000
VERSION     DAZKIP010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.045"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(59..910)
                     /locus_tag="ACHOUK_002318"
     CDS             complement(59..910)
                     /locus_tag="ACHOUK_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorothioated DNA-binding restriction
                     endonuclease"
                     /protein_id="HFP9897767.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLNGHPR
                     LFDYGTEIYEPLHSLLERFGPQRSQYRPDMPFWRLQGDGIWQLHNAERCSTAGSSRQP
                     PVKELNEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QTRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALEAAHIKWKQHGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGIVERLFWDFDGKTIALPQVRKNYPY
                     EGFVGER"
     gene            complement(984..1226)
                     /locus_tag="ACHOUK_002319"
                     /pseudo
     CDS             complement(984..1226)
                     /locus_tag="ACHOUK_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid mutarotase"
     gene            complement(1451..2554)
                     /gene="zorE"
                     /locus_tag="ACHOUK_002320"
     CDS             complement(1451..2554)
                     /gene="zorE"
                     /locus_tag="ACHOUK_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II Zorya anti-phage system protein ZorE"
                     /protein_id="HFP9897768.1"
                     /translation="MKLSIDISELIQLGKKMLPEGVDFFLDESPIDFDPIDIELSTGK
                     EVSIEDLDPGSGLISYHGRQVLLYIRDHSGRYDAAIVDGEKGKRFHIAWCRTLDEMRH
                     KNRFERYHATNRIDGLFEIDDGSGRSQDVDLRVCMNCLERLNYKGSIDKQRKREIFKS
                     FSLNEFFSDYSTCFRHMPKGIYDKTNSGYVENWKEISKEIREKANYVCNDCGVNLSTA
                     KNLCHVHHKNGIKYDNHHENLLVLCKDCHRKQPLHEGIFVTQAEMAIIQRLRSQQGLL
                     KAESWNEIYDLTDPSVHGDINMMQHKGFQPPVPGLDLPNSEHEIIATVEAAWPGLKIA
                     VNLTPAEVEGWRIYTVGELVKEIQTGAFTPAKL"
     gene            complement(2558..3265)
                     /gene="zorB2"
                     /locus_tag="ACHOUK_002321"
     CDS             complement(2558..3265)
                     /gene="zorB2"
                     /locus_tag="ACHOUK_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II Zorya anti-phage system protein ZorB2"
                     /protein_id="HFP9897769.1"
                     /translation="MDKIIGKQLPKKDQDNEHWVSMSDLMAGLMMVFMFISIAYMHYV
                     RIEKEKIKEVAVAYENAQLQLYNALDIEFAKDLQDWDAEIDKQTLEVRFKSPDVLFGL
                     GSTELKPKFKLILDDFFPRYLKVLDNYQEHITEVRIEGHTSTDWTGTTNPDIAYFNNM
                     ALSQGRTRAVLQYVYDIKNIATHQQWVKSKFAAVGYSSAHPILDKTGKEDPNRSRRVT
                     FKVVTNAELQIRKIIQE"
     gene            complement(3272..4924)
                     /gene="zorA2"
                     /locus_tag="ACHOUK_002322"
     CDS             complement(3272..4924)
                     /gene="zorA2"
                     /locus_tag="ACHOUK_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II Zorya anti-phage system protein ZorA2"
                     /protein_id="HFP9897770.1"
                     /translation="MLAQLFEQLFQSIDSTLITNIFIWAVIFVFLSAWWCDKKNIHSK
                     FREYAPTLMGALGILGTFIGIIIGLLNFNTESIDTSIPVLLGGLKTAFITSIVGMFFA
                     ILFNGMDAFFFANKRSALAENNPESVTPEHIYHELKEQNQTLTKLVSGINGDSEGSLI
                     AQIKLLRTEISDSSQAQLANHTHFSNKLWEQLEQFADLMAKGATEQIIDALRQVIIDF
                     NQNLTEQFGENFKALDASVKKLVEWQGNYKTQIEQMSEQYQQSVESLVETKTAVAGIW
                     EECKEIPLAMSELREVLQVNQHQISELSRHLETFVAIRDKATTVLPEIQNKMAEVGEL
                     LKSGAANVSASLEQTSQQILLNADSMRVALDEGTEGFRQSVTQTQQAFASMAHDVSNS
                     SETLTSTLGETITEMKQSGEEFLKSLESHSKELHRNMEQNTTNVIDMFSKTGEKINHQ
                     LSSNADNMFDSIQTSFDKASAGLTSQVRESIEKFALSINEQLHAFEQATEREMNREMQ
                     SLGNALLSISKGFVGNYEKLIKDYQIVMGQLQALISANKHRG"
     gene            5136..8495
                     /locus_tag="ACHOUK_002323"
     CDS             5136..8495
                     /locus_tag="ACHOUK_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4851/ECs_5259 family protein"
                     /protein_id="HFP9897771.1"
                     /translation="MLVKTPVNPLLQWLNMFFSRRSLSGADGRALYAYRCTDTEYESL
                     AELLRTYAPRSYPRTIFISYSDVLFSIYAAEFIRRTHTVGHPKWDTILDSINWKVPYV
                     HRQKLVNDGIRYWKRKIRNLGQASGYLHTLACEGGLPIRMIENESGYLITYFRRIYQA
                     LRGQSSQYPAAKIAQELGDTIPVTMQNELVYEIAGEFCETLCRLLSEHPPHSSDPVSA
                     LRKLSPDWHLQLPLVLPEANAAEIVRRLLSQSSEIRSASSLQVERIWVDVDDSWYCDA
                     RFRFPATMRTEQLTSLFECHIQPEQTRLIISGKWKNGGARLAMLSRYEQQDWRVELLP
                     IAMQKLSGADAMAEISLSLHEGPILLGHTIPKGGYELTEELPWVFEAMNESESQLKLV
                     GMGSVSSRLNALFISLPKNSHLDISGEGEFDIPRLLKNSERSLTKISGVFSVVLHDGA
                     VCTIRTQQLYDSAIEYYIKSTEVELVKSDYPVHRAWPKIGWKKDLQYGIVPEKELFWR
                     SIRSGNNAWYSVASEMPKGQIEVRRIVNDEVLFSGKVVVLPADFDINIIPESAQQGII
                     MLSGITDTRIDKYSNNEKVTLKSDYSQNECAIYYNSSLMLENTVDLRVSWKDGSNLKL
                     LLPKPVSGGRFVTNDGSVHFDGVASIAHLHGIDAELLTISCAGRGYLNIELLDENPVA
                     EKFRYLHADLPLLSGRNDKLQQISLYENYNLLNAMLACAWNSNSTLCVDFYSDRFGKD
                     KATLNIKRYDGSFIEHDQGLLVDIKNSVVFPANRIDELVVDAISLKNPGLHISLLKKD
                     EFAYDLSALNVQDSPWLIVGKLDGTARIAPVIKWMLPVLQTNDLLLNALCEADPEQRK
                     KNFNELIFEIDNNPLQNYCCLLTEYIKKYKMNNGLSLLDLDLFRGISSNYRVVVQLLI
                     SSCLSGDSDTIYDIQEELPFSWGWIPVSIWKDVFQKCWTYLEKQINDKTLALHILQPF
                     IAFMNHRAHIDRRLAPIANMLLTYSESLPTGCDVLPTVSREQFNEAKQMLLRNPDSFG
                     RISIFPKELWSSAITPELKSVFNKLWIEDKYHSRLEKRFNLMLVAALLTQKDNNLIHQ
                     LSALFEFHYQQAPQQLGVIYQYYFEQAGVCH"
     gene            8495..14809
                     /locus_tag="ACHOUK_002324"
     CDS             8495..14809
                     /locus_tag="ACHOUK_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP9897772.1"
                     /translation="MDVQQLHYAPLLNQLHQRAVRSVVSQLALRSKSLTHYLESQYFQ
                     PPGQEGSLLADPVFESTFGWMPADETMEDLRDKLISSPLVDALSKAPVPFRHQLTAWR
                     TILEDKKSLLVSSGTGSGKTECFMVPVLEDLIRQQKSTRQSLTGTQAIFLYPLNALIN
                     SQQERLREWTRGFKGKIRFALYNGETRHTKYEIQEDQLKVPEQALSREAIYEAPPSIM
                     VTNTTMLEYMLIRRKDAPIIEKSQGMLKYIVLDEAHSYIGSQAAELALLLRRVMQAFN
                     VGPGTDKPVQIIATSATIGEDSPEGNKELAKFVADLAGVTERDVKVVRGYRQIPRVSE
                     SLIRHEYPTSLTSLKSLSPQDLYQQLCHYRVAQQLRQALTHPGRQAVRLSELLNVARR
                     TWPDINHRELLQLLDLMARSREGELAFTPLRMHGFIRTLAGLWACSNKQCSHKAHELN
                     QSDWPFGQVWFEQRQYCDCGAPVFEVLRCSGCGSAYLSAKEEMRGDGTNWLVAQPAAA
                     EVDEFALDVDVYSEDEDNDELDNNSQFDRLIASDGEKYIISLEETTAGKIDSEAQKHY
                     EINLLRPESRGEGRNNSFACVCCGDTQRKNNPLFRPLRLGAPFFLNEIIPTLLEFSPL
                     PQTREEQLGPFNGRRLLTFTDSRQGTARISARLQQDADKATLRALCYQELANITEPSA
                     KLSPAHCALLEKVISQFSVLPLKNSIDALKKAMAANEELSPTDIQALKIIEPMVSSLS
                     TEHAEAMQILQANSGNQQKKTMSWQTLSEQLVGSVDLADRMRGSFKELSGLDLTKQQF
                     ADFCLYSEFGRRPKNAWTLESLGLVAIHYPFIDKVTTCPQDWKTLLPDPDKQLAEWRN
                     LLKITLDFFIRENSAVFYENEQYPRWMGARFPVKMLQGPDKKNKGQKRDQLWPQIRDR
                     HYNRVIKLLIAVFPAIQPEQAHWKSLVNHLMIEVWDAIRPCLRQFESGYQLDIKQQAE
                     FYSPKQVWRCPYTRRALDVTLLGYSPYLPGSKEIAPEKAVLIEMPELPVRHWRLSGGG
                     EIAREERLEWLESNALIQHAREEGLWSTRSDRLALKDSWYRLEEHSAQRTPEQNQFNE
                     KQFKSGKVNVLNCSTTMEMGVDIGGMSLVAMNNVPPAPANYLQRAGRAGRRGESASAA
                     ITLCKNTAHGMEVFKDPLWAFNTTASAPRVRFGSSSIVQRHVNALVLGLFLRAEVPDA
                     TKLSCKWFFEGDESQCLRFLHWLNHQADQLADKLKRLTQGTVLMSLTATQLLTRTQAM
                     MQQVDIRWRSQLAILLENIEALKADNSAWEETPAGKAIAYQLRDYRGAYLFSKLISEA
                     FLPGHGFPVGVVNFNYLTADELEKRRAIKATQADPNEGGESFSRRIEKLPSRDLPTAL
                     REYAPGADVVLGGKVYRSSGIMLGKVLASGQELSGDHHIPWFWHCRKCGAGATSTTHP
                     VECSHCKADIQQLDVKRYLQPVGFATDIRYQAHNDVSMPAQLPWKDPRVLVPSSVWVS
                     LPDAGLGRYRFSHSGELFHFSEGEFGHGYAICLSCGRAESQTQPQRTPENLKNPEREN
                     THYLLRGGSNDRQGSNKLCHGHVHKDLWLGYSSRTDMVELQLNDDNGLLIRDEVAARS
                     LAVALREGLAHKLGIENTELGVTTQQARDINGYTGYSIFIYDNNAGGAGYAVQLIDHW
                     ADVFNYARKLLDCSCDKFCHHCLLSYDSQHYVNRLDRHHALTLLTNVRLQRLNLAPEY
                     QYFGDGSRVETNPLSLRIAQCLNSEIYDSCSLVLAGPQEQWDFAQWPLFKELLQFASS
                     GGNVELLVATPLANLTDSSRHQLSALAAMPGGRLQVKSIATAQLMQGKGRWLAQVTRE
                     GQSQQWAADDSATVAPGELWGQSASSPVVTLKGTSGKTFSGQTLSAEDLLPALPTGAV
                     RINLCEQLDGPLEGFGSRFWSLVTQQHAGWKQAFTRHKEITHVEYSDRYLNSPFTARL
                     LGEILTELVEQGMAERASLTVCVKKLDYNSRQHDALYNAWLNEEDRQQVVTTLLEEGY
                     LGPAWPGAISWLTGDNQSTEHGRELTVTFSDGSQHYVLLDMGLSYWRCIEDTFFDFAL
                     RVPQQVERLANTRARAVAPGNDLRSYIIAG"
     gene            15033..17891
                     /locus_tag="ACHOUK_002325"
     CDS             15033..17891
                     /locus_tag="ACHOUK_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP9897773.1"
                     /translation="MNKQNYAPGMRVVIRDAEWRIRRADDSGDGGYLLTCDGISELVR
                     GKEGLFLTKLEQKVEILDPAKTHLVEDESANYQAAQLYIESQLRQRVPTDSKVHFGHL
                     AAMDSMPFQLDPTRMALAQPRQRILIADAVGLGKTLEAGILVSELIRRGRGKRILVLA
                     VKSMLTQFQKEFWSRFAIPLTRLDSAGLQKVRNRIPTNHNPFHYFDKTIISIDTLKQD
                     IEYRHHLENAWWDIIVIDEAHNVAERGTSSLRSKLAKLLAGRSDTLIMLSATPHDGKA
                     ESFASLMNMLDPTAIANPKEYEYADFADKNLVVRRFKKDVKDQMSGEFPERNIVKLTR
                     LASGAEEEAYRRLVESQFRDDDDEQAQSNKGRLFKITLEKALFSSPMACASVVANRLK
                     RLESRKDHNSQSQINELESLLLALNNIDASQFSKYQLLLDTIRKDLAWKANNTEDRLV
                     IFTESIKTLEFLEQQLRADLKLKDDQIATLRGDQGDTVLMETVEAFGKTQSPLRLLVC
                     SDVASEGINLHHLSHKMIHFDIPWSLMVFQQRNGRIDRYGQKHQPQIRYLLTEASEPQ
                     INGDMRVLEVLINKDEQAQKNIGDSSEFTGKFTQEEEEEQVAEFMMQDDGASLFDQLL
                     NSNVSESAEHDLFGEICSAVSSDASMVTETDTSLFASEQAYCERALGYLKASGQTIQY
                     ETLPDNTLSLVAPEELRRRFNQLPPEIAPENWQLYLSQDKTVITDAIARARGEQHAWP
                     DVQYLWQINPVVQWLDDKISSAFGRHQAPVIRLPYLLEPDEDHFILSGLFPNRKSHPM
                     VNPWIVVSFNRESLIGSQPFAEFLQRHPQLSNKLTNSGGKDRNHQRQQDLLEAAIAHA
                     REVFIHDRNAFETHINQQLNEHLQKLDVLRGRQLSQLELDFADNKQQLSVKQSRKEQR
                     QREIEHNFDSYIEWIEDTMTTEKEPYIQVIAVITGAEG"
     gene            17894..22828
                     /locus_tag="ACHOUK_002326"
     CDS             17894..22828
                     /locus_tag="ACHOUK_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9897774.1"
                     /translation="MALVGINNENEFYSNHYLGEVFTSDIRDVLEPWIAQENAAREAE
                     RAAREQGKDVEPGYRAPWNQFNSLATEFFRKLAEHEKQRQIPQRLADQRNRWQPLLKA
                     LGYEITPQIQMLDDDTPLPVLARYNSTDGSPWLWIVEAHDQEEGTLDPLALSLLTAQF
                     PADTDKHKHDSLRKKANGEYRSWQDLLSTAVFTQNEPPRFVLLLGNRQLLLLDRTKWA
                     QNRLLRFDFEEILSRRETDTLKATAVLLHKDSLLPGSGAPYLDSLDDNSHKHAFGVSE
                     DLKYALRESIELLGNEAMHYLIDRGLANYTGNRAVDPDELSRECLRYMYRLLFLFYIE
                     ARPELGYAPMTAKTYLQGYSLETLRDLEMIPLTSEEDRNGRYFHDSLNMLFKLVREGY
                     NGGVKMQSDLESGDRITIHSHQFSVPRLESHLFDANNTRILNRVVFRNETLQQIIQAM
                     SLSRPAKGRFNRRGRISYRQLGINQLGAVYEALLSYRGFFASEDLYEVKKAGEEFNEL
                     ETGYFVSKDEISKYHEDEKVYEKDGSLRIHRKGSFIYRMAGRDREKSASYYTPEVLTR
                     SLVKYALKELFKEQIDPISDPHAKADAILNLTVCEPAMGSAAFLNEAINQLAEAYLFH
                     KQQAEGRRIPQDRYTQELQRVKMYIADNNVFGVDLNPVAVELAEVSLWLNAISGDAFV
                     PWFGYQLHCGNSLVGARRQVFNKSELTYKKAKDPSWLNSEPVELAMNTPREETQIFHF
                     LLPDGGMANYSDKTVKQRYPDDFKALDSWRKEFIKSFAGHEIADVQRISEKVEALWNT
                     YRQQLKAERLKTADSYPVWPAENSEQTRSSLSSKDETFSGRLEDNSAYQKLRWVMDYW
                     CALWFWPIDKADELPDRGTWLFEIETLLDGIVITEKVTEVAEHTTGDLFAEEGLLREE
                     SSLFSVAGRLKTEVLFRHLPRLAIVDALRKQHRFFHWDLEFCDLFAERGGFDLMLGNP
                     PWLKVEWQEAGVLGDYEPEFVLRKLSASKLATLRIDTFNQIPALEAAWRSEYEGCEGM
                     QNFLNAQQNYPVLRGVQTNLYKCFLPQAWRLGAQKGVAGFLHPEGIYDDPKGGQLRAA
                     VYPRLRAHFQFQNELNLFVEVDHHAKFSSNIYSASPSTVGFEHISNLYAPQTIDACFE
                     HSGSGDIPGLKDEIESEGKLKVVWNTSGHRSRLISIATHELELFARLYDSEGTPAWQA
                     RLPALHAKQLVAVLEKFANQPNRLGDLQGQYFSTVMFDETYAQRDGTILRQTQFPQDS
                     SQWVLSGPHFFVGTPFYKTPRENCTLNSDYDCLDLLTLPDDYLPRTNYIPACDAQEYA
                     KRTPCVTWTELAEDEPKKVTDYYRLAIRAMLAQSGERTLISAIYPPEISHMNAVRSYC
                     YSSQNLLLEHSGMCFSLPFDFICKSTGKANLHQMLDGFSYVLFNPRQKALLYCLVLSL
                     NSVNDVYAGLWQSCYTPDFNTQRWSRDLPQLPQDFFAKLTPEWQRNCALRSDYSRRQA
                     LVEIDVLVAQALGLTLEELLTIYRVQFPVMRQYEADTWYDQNGRIIFTPSKGLVGVGL
                     PRTARKADLKNGFVFNVDSPEWTGGDCTDQAIGWDDVKHLKTGTVSVTFDDYTRSDEG
                     ERRTVTWQAPFIKPDREDDYKVAWAFFAQDKESA"
     gene            22828..29169
                     /locus_tag="ACHOUK_002327"
     CDS             22828..29169
                     /locus_tag="ACHOUK_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP9897775.1"
                     /translation="MLPSVVSRQVADSVAAFLRAAFPLNSPLFNGENNDNVSMLEQFL
                     AQPETLLKGPYLSAQLPFRKSDLPLNFFPNLTLPFPPHAHQARAFQRLGSDAPQPTLV
                     ATGTGSGKTECFMFPLLNHCAGASQAGVKAIIIYPMNALATDQASRFAKTIASDPQLH
                     GKVTVGLFVGDSEIEPSKKMSAEKVITCKHTLRESPPDILLTNYKMLDYLLMRPGDQP
                     LWRYNQPGSLRYLVVDELHTFDGAQGSDLACLVRRLKHHIGVDNQRFACVGTSATVGD
                     ELGQLLDYAKTIFDQPFTDDAVIREDRYTAAEYLQNYAIAYSQYPGPEVASALDPQSY
                     ATPVAYLNGQIPLWFPDSDLQLPDDLESDDGREKRIALGSLLRRHSVMHVLLHDLQGG
                     ILSERECLENLQVMLAESADHARRVLQSILALIAQARLEVPETEHDRQKRLQAKKARP
                     VLPFVQLRSQLWLREMRRLVASVAKTPRLVFADDVAVEDREHYLPVIHCRDCHATGWA
                     SLRHGQSVQLETDLDGIYRQFFEKGRSIVLAFPDNNEKAVSGAHQKLCPTCLKLNRQS
                     SVQCGHCGHTELLQVLIPDMLKERKDELEFANECPYCNSKQGISILGSRAASLSAVMI
                     HQLYGSQFNEHKKLITFSDSVQDAAHRASFFTARTWPLMIRGQIASVLSEKETLPLDA
                     FAARVCQQTRERSPDDAHFAANVIAPNMQWLNDYQTLKNEGKLAENSDLPELVSLRLD
                     WEVYSEFTLRARIGRTLEATGYAVAGVDKDRFNPAITALHQQLCEELGDEMSGVSVEQ
                     VAHLALGVIWYQRQAGAVMHPAFESYRRDGTTFMMTQKERTSRYMPSIGPKTRKPAYA
                     SFEKINGFHRLIGAKSNPSWYQHWINRTLSNGNNLFISSVAETVLRRLFTALKIAGVV
                     KDFDTKGREAWGLVPSALVVSRDVVQLNCSCCREVVRAPADQGWQWRNAPCLSLRCEG
                     RYQEVDNTATWRWENMDIARVQGAEHTGLLSREDREVTEKSFYRGNQPWNINLLSATP
                     TLEMGIDVGDLSTVLLCSVPPAQANYLQRIGRAGRKDGNALNITVAEGNPHDQFFFEE
                     PLEMMQGQVQAPGVFLNATAILERQLAAFCMDNWVKTGVPESAICKNVKQMLDELEFG
                     RKSGFPYNLLRYIEQHHAEIAAQFTAIFPDLAAETRQQLLSYLQGAPGQRSLVQRIEE
                     ALKLLVEDRKSLRSRIDKLKRSIDKLENAPRDQNFDSDMRELTSERQALMALVNQINN
                     KQTLNFLTDEGLLPNYAFPEAGITLRSVLWRRKEGGEAREYQNTTFEYERPASTALAE
                     LAPLNNFYAGGHKVEIEQIDLKVSEPENWRICSHCNYSENIDQTGDQHKYCPKCGTPG
                     WADAGQKTTLLKLRQVYARASARDSQISDESDSREPAFFQRQLLVSFEKEDVSAAYAI
                     EEGEVPFGFEFLSKVTLRDINFGKMADDANELMIAGEAKKRTGFKVCLGCGMVQRPRD
                     HEPRHDLSCKYRTEPEKAKFEDYLYLYRQLESEALRILLPVSSYSNDRVVEASLGAAI
                     QLGLKHYFKGNVDHLKGVVYREPENEGESWRQYLVIYDTVPGGTGSLKELMRTPDNLL
                     KLLELAYQAIVECSCNNDTHKDGCYRCVYAYRDRGRMKYVSRDQARLLLAKILQARDS
                     IRVIDSIKNISLEAMMGSELEKRFISCLQENKNLIVSRSYAHQGAGWIINTRSEPMMS
                     WHLKAQVDLGAKEAVGIPCRPDYVLYPLMQSDAIKPVAIFLDGFAFHKNSVAEDVQKR
                     QAIRDSGNFWVWTVTWADLKEPGLKHVQDVLGLGHNPDMKQPKFYNLFHDTNFAALEA
                     SFRERNSFALLLDYLADPGGRTQLWQRMAAAHAWVWLDVKKSQDAGTKQKYAYEMQEN
                     APAWRLAELLPDEPFVFGGLLDSCNSSQQFIELASVLPQQAIKPTTSVAQMRSWLRLH
                     ICFDDRYTQDDGYEAGLNGFWRLVNLLQFLPDMTFTSRKAVHLPQESVDTKVSATPEA
                     VPETDESWAEIIEFGLLSAEEIALLQSHSLSAPTLGYELQNDEGEIIAEADLAWPSQK
                     WALIIDNQEFIPLFTTRGWHVAFGPIDENTLQHLSGGDK"
     gene            29166..31280
                     /locus_tag="ACHOUK_002328"
     CDS             29166..31280
                     /locus_tag="ACHOUK_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFP9897776.1"
                     /translation="MTVQTPKVALSDGFLGAFARIPKAQQKKVQEFISKFRQDPTSNG
                     LNYEKIHDARSKNVHSVRIDQTYRGIVLKPEQGALYMLMWVDKHDEAYDWARRHDCSI
                     HPVTGAIQVIDISYIKPAAETVVDKPKLFAAYSAEQILALGVPPVFIDQVMALTDEAG
                     LNQLESIMPAEAWEPLHWLAEGLDYQEVLEEFNDHRDEPVDTNDFMEAIERSKRRFHV
                     VENEQELLQMLNAPLEKWRVFLHPSQRKLVESPANGPVRVLGGAGTGKTVVAMHRARW
                     LSQRLADKPGKKVLFTTFTRNLAADIRANLQRLCTREEMARIDVVNIDAWMSDQLKRH
                     GYDFRVVYDSDEGRRKCWNYALQQAPAELGLPDNFYAEEWQRVVQPNAVYTREEYLKV
                     SRVGRGTALSRIQRAKIWPVFEEFRAQMARAKLREMGDAMHEAIVLFKEKQVQLPYSS
                     IVVDEAQDIGAPAFTLIRSLVPESPNDLFIVGDGHQRIYRNKVVLGQCGINVRGRRSK
                     KLKINYRTTEETRQFAVGLLTGVKVDNLDGEADTSNDYLSLLHGEKPMITHAADFKEE
                     AATLVKQIQALLANQVRSQDICITARTKHACDRYASALNDAGIETFNLGNDSGDSDAR
                     PGVRVATMHRIKGLEFQYVFLVGINEGVVPEIKALASDDPVEQRDALFNERALLHVAA
                     TRAVKGLFVSSSGVPSPLLVAD"
BASE COUNT         8317 a         7215 c         7940 g         8285 t
ORIGIN      
        1 catcatatct ctgatgcagg agcagagaat ggctataaat tattagtaaa cagaatgtct
       61 atctctcacc cacaaacccc tcataagggt aatttttacg cacctgcggc aacgcaatag
      121 ttttcccgtc aaaatcccag aataatctct caacgatccc tccgccgttt accgcatccg
      181 agaccagtac ccgcatattt tcatccaggc caatcgatcc tttatcaaag gctttatggt
      241 gtattgcgca aagcgccaga ccattgggga tttcacaagg gccgccgtgc tgtttccatt
      301 ttatatgcgc cgcttccagc gcgacggtgg tgtcatcgtg gcgcatatta aagccacata
      361 ttgcgcactg gtagttataa gcgcgtaata catttttgcg aaataacggg tcgcgctgtt
      421 tacgggtttg ctggagatca aaacccagct cgtcagcgat ttcttcctga atactctcgg
      481 tgaagtgcgc ttcgagtatc tgttgcgcca gagtattgat gagtttttta ttgccggtta
      541 ccagggcgta gtgctgttca tcgaaaccgc ctgcaacgtg gtattcgttc agttccttca
      601 ctggcggttg tcggctgctc cctgctgttg agcaacgctc ggcattgtgt aattgccaga
      661 ttccgtcgcc ttgtaatcgc cagaacggca tatcaggtcg gtactgtgaa cgctgtggcc
      721 caaaacgttc cagtaaactg tgcagaggtt cgtagatttc cgtgccataa tcgaaaaggc
      781 gcggatgtcc attcaggtat cccgctaata cgtatagcag taacaatggt ttatgcggcg
      841 cgcgctgttc acccttgtgc cagattttta tgttggcaat tgcctgctgt agcgatttac
      901 tggaagccat tgatatccca ggcagtgaaa taattgcaat catgctcgca aattttctgc
      961 tgttcaagcc tgcaagtgaa gatccattgc tgtgttgagg gatcaaaaga caacaaaaac
     1021 ttattgaaga aataatcttc tgcttttttg tcgaaataat gggcattgat cttgtctata
     1081 gctgctgaat cttttccagc ctcgttgaga tcttcaaaat agccattgaa gatgttctgg
     1141 ttaacaccgc cagtaacata agccttgccg ttgtgtacaa aagtcacatt acccgccatg
     1201 cccatcggcg cgtgcgacat caatttattg ctttattcat tgtgactgtc ttctgtctac
     1261 caagctttaa ccttcaccca tatgagggaa gttttaagta tgaggcgaac catagcatat
     1321 gcttttatac ctgttacacc ataaaaatca gacaagttat gccataaact acttttgtaa
     1381 tttgagccac aaatcaggga aattttattt atcttccgtg aaagtcttaa ctttcacgga
     1441 gttttagaat ttacaatttt gctggcgtaa aggctcctgt ttgtatttct ttaaccagct
     1501 cacccacggt atatattctc catccttcga cttcggcggg agtaaggtta actgcaattt
     1561 taaggcctgg ccatgcagct tctacggttg caataatttc atgttctgag tttggaagat
     1621 ctaacccagg aacaggaggt tgaaagcctt tatgttgcat catattaata tcaccatgca
     1681 ctgatggatc agtcaggtca tatatttcat tccaggattc tgcttttaat aacccttgtt
     1741 gggaacgtaa acgttgaatg atagccatct ctgcttgggt aacgaatata ccttcgtgga
     1801 ggggctgttt tcgatggcaa tccttgcaca gaacaagaag gttttcatgg tgattatcat
     1861 atttgatgcc atttttatga tggacatggc acaagttttt ggcggttgat aaattcacgc
     1921 cacaatcatt acaaacataa tttgcctttt ctcgtatttc tttagatatt tccttccagt
     1981 tttcgacata cccactattt gttttgtcat agataccctt aggcatatga cgaaaacaag
     2041 tactataatc tgaaaaaaac tcatttaatg agaatgattt aaaaatctct ctttttcgtt
     2101 gtttatcaat acttccttta taattaagtc gttcgaggca attcatacat acccgtaaat
     2161 caacatcctg gctccgacct gaaccatcat caatttcgaa taaaccatct atgcggttag
     2221 ttgcatgata cctttcaaat cgatttttat ggcgcatttc atcaagagtt ctgcaccagg
     2281 caatatgaaa acgttttcct ttttcgccat ctacgatagc cgcatcataa cgccctgaat
     2341 ggtcccgaat atataaaaga acctggcggc catgataaga gataagcccg ctaccagggt
     2401 caagatcttc gatactaact tctttacccg tggataactc aatatctata ggatcaaagt
     2461 caatagggga ttcatccaga aaaaaatcga ctccttctgg taacattttc ttccctaatt
     2521 gaataagttc tgaaatgtcg atagataatt tcatctctta ctcctgaata atctttctaa
     2581 tctgcaactc ggcatttgtt acaactttga aggtgacacg acgagagcga ttaggatcct
     2641 ccttgccggt tttatcaaga atgggatgtg cagatgaata acctactgcg gcaaatttac
     2701 ttttaaccca ttgttggtgt gtcgcgatat tttttatgtc ataaacgtat tgtaatactg
     2761 cacgtgtacg accttgcgat agtgccatat tattaaaata agcaatatca ggattcgttg
     2821 ttcctgtcca gtcagtactt gtgtgacctt caatgcggac ttcagtaata tgttcctgat
     2881 aattatctag aacttttagg tagcgaggaa agaagtcgtc aagaatgagt ttaaactttg
     2941 gttttagctc tgtgcttcct aagccaaata aaacatccgg tgatttaaat cgaacctcca
     3001 gagtctgttt atcgatctct gcatcccagt cttgtaaatc ctttgcaaac tcaatatcca
     3061 gagcattata aagctgtaac tgagcattct cgtaggctac ggcaacttct ttaatttttt
     3121 ctttttcaat acgtacgtag tgcatataag caatagatat gaacataaaa accatcatca
     3181 gcccagccat taggtccgac atggataccc aatgttcatt atcttgatct tttttaggta
     3241 attgtttccc tataatctta tccatgatcg attacccgcg atgtttatta gcagaaatta
     3301 atgcttgtaa ctgccccata actatttggt aatctttaat aagtttttca tagttaccga
     3361 caaaaccttt gctgattgaa agcagagcat tacctaatga ttgcatttca cggttcattt
     3421 cacgttcagt tgcttgctca aaagcatgta actgctcgtt gatggataga gcaaattttt
     3481 caattgattc tctgacttga gaagtcagcc ctgcacttgc cttatcaaat gatgtctgga
     3541 ttgaatcaaa catattatcg gcattactgg atagttgatg gttaatcttt tcaccagtct
     3601 tactgaacat atcaatcaca ttcgtcgtat tttgttccat atttctatgc aattctttcg
     3661 agtgcgactc tagtgatttc aggaattctt caccactttg tttcatttca gtaattgttt
     3721 cacctaacgt gctggttagg gtttcggagg aattgctgac atcatgtgcc atcgaggcaa
     3781 atgcttgttg tgtttgggta accgattgtc tgaatccttc ggtaccttca tccagggcaa
     3841 cgcgcattga atctgcatta agaagtattt gctggctggt ttgctcaaga gatgcactaa
     3901 catttgcagc tccggatttc agcagttcac ccacttcagc cattttgttc tgtatttcag
     3961 gtaatacggt tgtagcttta tcgcggatgg cgacaaaggt ttctaaatgg cgggagagtt
     4021 cgctgatttg atgttggttc acctgaagca cttcacgcag ttcagacata gccagaggaa
     4081 tttctttaca ttcttcccaa atccctgcaa ccgcagtttt tgtttcaacc agggactcga
     4141 cactttgttg atattgttct gacatctgct caatttgcgt tttataattt ccctgccact
     4201 caacaagttt ttttacagag gcatcaagag ctttaaagtt ttcaccaaac tgttcagtta
     4261 aattttgatt aaaatcaata atgacttgtc gcaaagcatc aataatttgt tctgtagcac
     4321 cttttgccat tagatctgca aattgttcaa gttgttccca aagcttatta ctgaaatgag
     4381 tgtgattagc taattgtgcc tgcgaggaat cgctaatctc agtacgtagt aattttattt
     4441 gagcaataag agaaccttca ctatcaccgt taatacccga gactaattta gtcagagtct
     4501 ggttctgctc ttttaattca tgatagatat gttcaggtgt aacagattca gggttatttt
     4561 ctgctaacgc acttcgttta ttggcaaaaa agaaagcatc cattccatta aataaaatgg
     4621 caaaaaacat acctacaatg cttgtaatga atgctgtttt taggccacct aataatacgg
     4681 ggatgctggt atcaatactt tcggtattaa aattgagtaa accaataata ataccaatga
     4741 aagtacccag aatacctaat gcccccatta aggttggagc atattctcta aacttactat
     4801 gtatattttt tttgtcacac caccacgctg ataaaaatac gaatataaca gcccagatga
     4861 aaatattggt gatcagtgta gagtctatcg attgaaacaa ctgctcaaaa agctgcgcta
     4921 acattttgat acctgtgtaa ataatggata gttgtaatcg tttaaaaaat aaacaaaaca
     4981 cacataattt atatggttaa gttcggtgta gcgctcatga ttgtaacaag ttttttcgag
     5041 atataaatga tttgtctcat ttttagcgag ttcatcgggt tgagcttttt tatgatgata
     5101 gtaacctgtt acgaattgta tcaaaggaag gcaggttgct tgtgaaaact ccagttaacc
     5161 cacttttgca atggcttaac atgtttttta gtcgccgttc attatccgga gctgatggac
     5221 gggcgttata tgcttaccgt tgtactgata cagagtacga aagtttggca gaactactgc
     5281 gtacatatgc cccccgtagt tatccaagaa cgatattcat ttcctatagc gatgttctat
     5341 ttagcatata tgccgcagag tttatccgcc ggactcatac ggtcggacac cctaaatggg
     5401 acacaatttt agattctatt aactggaaag ttccgtatgt tcatcgacag aagctggtta
     5461 atgatggcat tcgctactgg aaaagaaaga taagaaacct gggacaagct tcgggttatc
     5521 tgcatacact ggcttgtgaa ggtggtttgc ctatccgcat gattgaaaat gagagcggtt
     5581 atttgattac ctatttcaga aggatatatc aggcgcttcg tgggcaatca tctcaatatc
     5641 ctgccgctaa aattgcacaa gaattaggcg atacgattcc tgtcacaatg caaaacgaac
     5701 tggtctatga aatagccggt gaattctgcg agactctctg ccgattactt agtgagcatc
     5761 ctcctcatag cagtgatccc gtttctgcat tacgtaagct ctctccagac tggcatcttc
     5821 aacttccact cgttctcccc gaagcgaatg ctgcagagat tgtaaggcga ttactttctc
     5881 aatcttctga aatacgcagt gcaagtagtc ttcaggttga aagaatctgg gtcgatgttg
     5941 atgacagttg gtattgcgat gcccggttcc gattcccggc cacaatgcgt acagaacagt
     6001 taacctcttt gtttgaatgc catattcagc cggagcagac ccgactcatc atttcaggaa
     6061 aatggaaaaa tggcggggca aggttggcaa tgctaagccg ttatgagcag caagattggc
     6121 gggtcgagtt attacctatt gcgatgcaaa aactctctgg tgcagatgca atggccgaaa
     6181 tctcattgtc gcttcatgaa ggtccaattc tgttaggtca cacaattcca aaaggcggtt
     6241 atgaacttac tgaagaactt ccctgggttt ttgaagcgat gaatgagagt gaatcgcagc
     6301 taaaacttgt tggtatgggg tctgtgagtt ccaggctaaa tgctctgttt atttcgctac
     6361 cgaaaaatag ccatttagat attagtggag aaggtgagtt tgatatccca aggttgctga
     6421 aaaacagtga acgcagctta actaaaataa gtggagtatt tagcgttgtt ttacatgatg
     6481 gtgctgtttg tacaattcgc acccagcaac tttatgattc tgcgattgaa tattatatta
     6541 aatcgacaga agttgaactg gttaaatcgg attatcctgt ccatcgagcc tggccgaaga
     6601 tcggttggaa aaaggatctg caatatggca ttgtaccgga gaaagaactt ttttggcgtt
     6661 ccattcgttc aggtaataat gcctggtatt ctgtcgcatc agaaatgcca aaaggacaga
     6721 tagaagtccg gcgaatagtt aatgatgagg ttttatttag cggtaaagtt gtagttttac
     6781 cagcagactt cgatattaat attatacctg agagtgctca gcaaggcatt attatgcttt
     6841 ctggtataac ggatactaga attgataaat attcaaacaa tgaaaaagta acacttaagt
     6901 ccgattattc acaaaacgag tgtgctattt attataattc atcgctgatg ctggaaaata
     6961 ccgttgattt acgggtctcc tggaaagatg gttctaatct taaactatta ttacctaagc
     7021 cggttagcgg tggccgattt gtaactaatg atggttctgt tcattttgat ggtgtggcat
     7081 ctatagcaca tttgcatgga atagatgctg agttattaac catatcatgt gctggaagag
     7141 gatatcttaa tatcgagtta ttggatgaaa atccagtagc tgaaaaattc cgctatttac
     7201 atgccgacct tcctctttta tcaggacgca atgacaaatt acaacaaatt tcactttatg
     7261 agaactataa tttgctaaat gctatgctgg catgtgcatg gaacagcaat agtacattat
     7321 gtgttgattt ttactctgac agatttggaa aggataaagc aacactcaat attaaacgct
     7381 acgatggtag ttttattgaa cacgatcaag ggttactggt tgatataaaa aattctgttg
     7441 tttttcctgc aaatagaata gacgaactgg ttgttgatgc tatttctctt aagaatcctg
     7501 gcttacacat atcattgtta aaaaaagatg agtttgctta tgatctttca gctctgaatg
     7561 ttcaggatag tccatggtta attgtgggaa aacttgatgg tacagctcgc attgcaccag
     7621 taattaaatg gatgctacct gtattgcaga caaatgattt attactaaat gctctatgcg
     7681 aagcagaccc agaacagcgc aaaaaaaatt ttaatgagct aatttttgaa atagataaca
     7741 acccattgca aaattattgc tgtttattaa cagagtatat taagaaatac aaaatgaata
     7801 atggcttatc cttgctggat ctggacttgt tcagaggtat ttcgagtaat taccgcgtgg
     7861 ttgttcaatt gttaatatca tcatgtcttt ctggtgatag cgatacgatt tatgatatac
     7921 aggaagaatt acccttttca tggggatgga ttcccgtttc aatctggaaa gatgttttcc
     7981 aaaaatgttg gacttatctg gagaaacaga ttaacgataa aacattagca ttacatatat
     8041 tgcaaccctt tattgctttt atgaaccatc gtgcacatat cgatcgtcgg ctggctccga
     8101 ttgcgaatat gttacttaca tatagtgaga gcctaccaac cggttgtgat gtattgccaa
     8161 ctgttagtcg tgagcagttt aatgaagcta aacagatgct attaaggaac cccgacagct
     8221 ttgggcgtat cagtatcttc cctaaagaac tttggtctag tgctattact ccagagttaa
     8281 aatctgtttt taataagctt tggattgaag ataaatatca ctcacggctt gaaaaacgtt
     8341 ttaatttgat gttagtcgca gcgctgttaa cccaaaaaga taataacctg atacatcaac
     8401 tgtctgcgct ttttgaattt cactatcagc aagccccaca gcaattaggg gtaatctatc
     8461 aatattattt tgaacaagca ggagtatgtc attgatggac gttcagcaac tgcattatgc
     8521 gccacttcta aatcagcttc atcagcgtgc ggtgaggtcg gtggtaagtc agttggcatt
     8581 gcggagcaag tcgttgactc attatctgga atcacaatat tttcagcctc ccggtcagga
     8641 ggggtcactg cttgcagacc cggtattcga aagtacattc ggctggatgc ctgcggatga
     8701 aacaatggag gatttgcggg acaagctgat ctccagcccg ctggttgatg cattgagcaa
     8761 agcgccagtg ccatttcgtc atcaacttac tgcgtggcga acgattctgg aagacaaaaa
     8821 atccttgtta gtgagcagcg gtacgggatc cgggaaaaca gaatgcttta tggtaccggt
     8881 tctggaagat cttattcgtc agcaaaaatc gacccgccag tcactaacag ggacacaagc
     8941 tattttcttg tatccgttaa atgcattaat taacagccaa caggagcggc ttcgcgaatg
     9001 gacgcgcgga tttaagggga aaatacgctt cgcgctatat aatggcgaaa cccggcatac
     9061 caaatacgaa attcaggaag atcagttgaa agtacctgaa caagctttga gtcgggaagc
     9121 catttatgaa gcaccacctt ccatcatggt gacgaatacc accatgctgg aatatatgct
     9181 gattcgtcgt aaagatgccc caatcatcga aaagtctcag gggatgttaa agtatatcgt
     9241 cctggatgaa gcccatagtt atattggttc tcaggctgct gagttagcgc tgcttttacg
     9301 tcgtgttatg caggctttca atgttggacc cggcacagat aaacctgtgc agattatcgc
     9361 gacctctgcc actattggtg aagactctcc tgaaggtaat aaggagcttg ctaaatttgt
     9421 cgctgatctc gcgggcgtta cagaacgtga tgtcaaagtg gttcgcggtt atcgacaaat
     9481 accgcgggta tcggagtcgc tgattcgtca tgaatacccc acatctttaa cttctcttaa
     9541 atccctgagc cctcaggatc tttaccaaca gctttgtcat taccgtgtgg cccagcaatt
     9601 gcgtcaggcg ttaactcatc ccggacggca ggcagtacgt ttgagtgagt tgctcaatgt
     9661 tgccagaaga acctggccag atattaatca ccgtgagcta ctgcaactcc tcgatctaat
     9721 ggcaagatcg cgagaaggtg aactggcttt tacgccatta cgtatgcatg ggtttatcag
     9781 aacactggcg ggtctatggg catgttcaaa caaacaatgc tcacataaag cacatgaatt
     9841 aaatcagagt gactggccat ttggtcaggt atggtttgag cagcgccaat attgtgactg
     9901 tggagcgccg gtatttgaag ttttacgttg ctccgggtgt ggttcagctt atttgtctgc
     9961 caaagaggag atgcgcggtg atggaacgaa ctggttggta gcgcaaccag ccgctgccga
    10021 agtggatgag tttgctcttg atgtagatgt ttattctgaa gatgaagaca acgacgaatt
    10081 agacaataac tctcaatttg atcgtctgat tgcgagcgat ggtgaaaaat atatcatttc
    10141 actggaagaa acgacggcgg ggaaaattga tagtgaagcg caaaagcact atgaaataaa
    10201 cctgttaaga ccggaatctc gaggtgaggg acgcaataac tcctttgctt gtgtttgctg
    10261 tggtgacact caacggaaaa ataatccttt attccgtccg cttcggctcg gcgcgccctt
    10321 ttttttaaat gaaattattc ctactctgct ggagttttct ccattaccgc aaacaagaga
    10381 agagcagtta ggaccgttta atggtcgacg cttgctcacc tttacggatt cgcgtcaggg
    10441 taccgctcgc atttcagccc gcttacagca ggatgccgat aaagcgacgt tgcgagctct
    10501 ttgttaccag gagctggcaa atattacaga gccttcagca aaactcagcc cggcacactg
    10561 tgcactgtta gagaaagtta ttagccagtt ttctgtcctc cctttaaaaa attccatcga
    10621 cgcgctgaaa aaagcgatgg ctgcgaatga ggagcttagc ccgacggaca ttcaggcact
    10681 taaaattatt gagccgatgg tgagttcctt aagcacggag catgctgaag cgatgcaaat
    10741 acttcaggct aactctggca atcagcagaa gaaaaccatg tcatggcaaa cgctgtctga
    10801 acagctggtt ggcagtgtgg accttgccga tagaatgaga ggcagtttta aagaattgtc
    10861 tgggcttgat cttactaaac aacagtttgc agatttttgt ttatacagcg agtttggtcg
    10921 ccgccctaaa aatgcctgga ccctcgaaag tctgggtctg gtagccattc actatccatt
    10981 cattgataaa gttacaacct gcccgcagga ctggaagaca ttattacccg atcccgataa
    11041 gcaattagcg gaatggcgta atttattgaa gattactctt gatttcttta tccgtgaaaa
    11101 cagtgccgtt ttttacgaaa acgagcaata ccctcgctgg atgggggcac gtttcccggt
    11161 aaaaatgctc caggggccag ataaaaaaaa caaagggcag aaacgtgatc aactttggcc
    11221 acaaatacgc gatcgccatt ataaccgggt cattaagcta ttaatcgcag tctttccggc
    11281 aatacaacct gagcaggctc actggaaatc gttagttaac cacttaatga ttgaggtctg
    11341 ggatgcaatt cgaccctgct tacgtcagtt tgagtcgggt tatcagctgg atattaaaca
    11401 acaagctgaa ttttacagtc caaagcaagt gtggcgttgc ccctatactc gacgtgcgtt
    11461 agacgtcacg ttgctgggat actctccgta tttgccgggt agcaaagaaa ttgcacctga
    11521 aaaagcagtg ttgattgaaa tgcctgagct tcctgtacgt cactggcgtt tgtctggtgg
    11581 tggagaaatc gcccgagaag aacgtctgga gtggctggaa agtaatgcgc tgattcaaca
    11641 tgcccgcgag gaaggattat ggtctactcg cagcgaccga ctggccctga aagatagctg
    11701 gtatcgtctg gaggaacact ctgcgcagag aacgcctgag cagaaccagt ttaatgaaaa
    11761 acaatttaag tcaggcaaag ttaatgtgct gaactgttcc acgaccatgg agatgggggt
    11821 ggatattggt ggtatgagtt tggtggcaat gaacaacgtt cctccagcgc cggcaaatta
    11881 tttgcaacgt gcaggtcgcg cggggcgtcg tggagagagt gcatctgctg ccattacgtt
    11941 atgtaaaaac acggcgcatg gaatggaggt attcaaagac cccctctggg cgttcaacac
    12001 tactgccagt gcgccacggg ttcgttttgg gagcagtagt attgttcaac gacatgttaa
    12061 tgccctggtt ttaggtctgt ttttacgagc tgaagttcct gatgcgacaa aactgagttg
    12121 caaatggttc tttgaaggtg atgagagcca gtgtcttcgt tttctccatt ggttaaatca
    12181 ccaggccgat cagctggcgg ataaacttaa acgccttact cagggaaccg tactgatgtc
    12241 attgacggca acccagctct tgacccgcac acaagcaatg atgcagcagg ttgatatccg
    12301 ctggagaagc caacttgcga tactgttgga aaatatcgaa gcgttgaagg cggataacag
    12361 cgcgtgggaa gaaacaccag caggtaaagc catcgcttat cagcttcgtg attatcgcgg
    12421 tgcatatctg ttttctaaac tcattagtga agcgttcctg ccggggcatg gtttcccggt
    12481 tggcgtagtc aatttcaatt atctgacggc agatgagctg gaaaaacgtc gcgcaataaa
    12541 agccactcag gctgacccta atgaaggggg agaaagcttt tctcgtcgta ttgaaaaatt
    12601 gcccagccgt gatttgccta cagcattacg cgagtatgcg cctggtgcag atgtggtatt
    12661 gggcggaaaa gtatatcgtt cctcgggcat tatgcttggc aaggtgcttg ccagcgggca
    12721 ggagctgtca ggcgatcatc atattccgtg gttctggcat tgccgtaaat gtggtgcggg
    12781 ggcaaccagt actacccatc ctgtcgaatg tagtcactgc aaagcggata tacagcaact
    12841 tgatgtgaag cgttatttac agcctgttgg ttttgcgaca gatattcgtt atcaggcaca
    12901 taatgatgta tccatgcctg cacaacttcc ctggaaagat ccacgtgttc ttgtaccgtc
    12961 ttcggtgtgg gtttctttac ccgatgctgg gctaggacgt taccgtttta gccatagtgg
    13021 ggaattgttc cacttcagtg aaggggaatt tgggcacggc tacgcaattt gcctctcatg
    13081 tggtcgggca gaatctcaaa ctcaacccca gcgtacccct gaaaatctaa agaacccaga
    13141 gagggaaaat acgcattacc tgttgcgagg gggcagtaac gatcgtcagg ggagcaataa
    13201 attgtgccac ggtcatgtgc ataaagatct gtggttggga tatagcagtc gtacagatat
    13261 ggttgagctg caactcaatg atgataatgg cctgttgatc cgtgatgagg ttgccgcgcg
    13321 ttcccttgcg gtcgctttgc gtgaagggtt agcgcataaa ttgggtatcg aaaatactga
    13381 gctgggcgtg acgacgcagc aagcgcgcga tatcaatggc tataccggat attccatctt
    13441 catttacgac aataatgcgg gtggtgcagg ttatgcagtg caattgatcg accattgggc
    13501 tgatgtcttt aattacgcca gaaaattgct cgattgcagt tgcgataaat tctgtcacca
    13561 ctgcttgctc agctacgaca gtcagcatta tgtcaacaga ttagatcgtc accatgcatt
    13621 aaccctgctt accaatgtac ggttgcagcg acttaacctc gcgccagaat atcagtactt
    13681 cggcgatggt agccgggttg aaactaaccc tctgagttta cgtattgcgc aatgcctgaa
    13741 cagcgagatt tatgactcct gctcgctggt tcttgcgggg ccgcaggaac agtgggactt
    13801 tgcccaatgg ccattattta aagagttgct ccagtttgcc tcctccggtg ggaatgttga
    13861 gttgctggtg gccacgccgc tggcaaactt aactgatagc tccagacatc agctttccgc
    13921 gttggcggcg atgccgggag gtcgcttaca agtgaaatcc attgccactg cgcagctcat
    13981 gcaaggaaag gggcgctggc ttgcgcaggt gactcgcgaa gggcaaagcc aacagtgggc
    14041 cgcagacgac agcgctaccg ttgcacctgg tgaattatgg ggacaaagtg cgtcttcacc
    14101 agtagtgaca cttaaaggaa cgtccggaaa aaccttctcg ggtcagacct taagtgcaga
    14161 agatttactg cccgccttac ccaccggggc ggtgcgcatc aatctctgtg aacaactgga
    14221 cgggccgctg gaaggttttg gttcgcgttt ctggtcgtta gtcacgcaac agcacgctgg
    14281 ctggaagcag gcatttaccc gccacaaaga gataacgcat gttgagtaca gcgatcgtta
    14341 tcttaatagc ccattcacgg cgcgtttgtt aggtgaaatc ttaaccgagc tggttgaaca
    14401 gggaatggcg gaacgcgcca gcctcactgt ttgcgtcaaa aaattggatt acaactctcg
    14461 ccaacacgac gctctctata acgcgtggct taatgaggaa gatcgtcagc aggttgtgac
    14521 cactttactt gaagaaggat atctggggcc tgcgtggccg ggagcaatta gctggttaac
    14581 gggggataac cagagtacgg agcatggacg agaactgaca gtcaccttca gcgatggcag
    14641 tcagcattat gtcctgctcg acatggggct tagctactgg cgctgtattg aagatacctt
    14701 ctttgatttt gccctgcgag taccgcagca agttgaacgg ctggcaaaca cgcgagcgcg
    14761 agctgttgcc ccgggtaatg accttaggag ttatattatc gctggataaa taattgctcc
    14821 cctggggata tcttccaggg gagttgaccc agctcacttt ttaataattt ttgtttgaaa
    14881 atcatgcgca atttatgcgc attccctccc attttgtcac tttgccgggg gccttcatga
    14941 tatctttaag gccctctttt ttatcgcagt gtgccgcgcc acatttttca ggatgacgtt
    15001 accgccactg ccccaaatag acagaaaaac cgatgaataa gcaaaattat gcacctggta
    15061 tgcgggtggt tattcgtgat gccgaatggc gtattcgccg ggcggatgac agtggtgatg
    15121 gtgggtattt gctgacctgc gatggtattt cagagcttgt gcgcggtaaa gaagggttat
    15181 ttctgaccaa actggaacaa aaagtagaga tcctggatcc cgcaaaaacg catttggtgg
    15241 aagatgaatc cgccaattat caagcggcac agttgtatat tgaaagtcag ttacgccaac
    15301 gcgtaccgac agacagtaaa gtccattttg gtcatctggc ggcgatggat tccatgccgt
    15361 ttcagctcga tcctacgcgg atggcgctgg cacaaccgcg tcagcggata ctcattgccg
    15421 acgctgttgg tctgggtaaa acgctggaag ccggtattct ggtttcggaa ctgatccgtc
    15481 gtgggcgcgg aaaacgtatt ctggttctgg cggttaaatc gatgctgaca caattccaga
    15541 aggagttctg gagccgtttt gcgatcccat tgacccgcct ggattctgct ggtttacaaa
    15601 aggtgcgtaa ccgcatccca accaaccaca acccgttcca ctattttgac aagaccatta
    15661 tctccatcga tacgctgaaa caagacattg aatatcgtca ccatctggaa aatgcctggt
    15721 gggatattat tgtcattgat gaggcccata acgtcgcaga acgtggaacc agttcgttac
    15781 gcagtaaact ggcaaaactg ctcgccgggc gcagcgatac gctgattatg ctttcagcaa
    15841 caccgcatga tggtaaggct gaaagttttg ccagcctgat gaacatgctt gacccgacgg
    15901 cgatcgccaa cccgaaagag tatgaatacg ccgattttgc cgataaaaat ctggttgtcc
    15961 gtcgcttcaa aaaagacgtg aaagatcaga tgtccggcga gttccccgag cgcaatattg
    16021 tcaaattgac tcgcctggca tccggtgccg aagaagaagc ataccgtcgt ctggttgaga
    16081 gccagttccg tgatgatgac gatgaacagg cccagtctaa caaaggtcgt ctgttcaaga
    16141 taaccctcga aaaagcgcta ttttccagcc cgatggcgtg tgccagcgtc gtggcgaatc
    16201 gtctgaagcg tcttgagagc cgtaaagatc ataatagcca gagccagatc aacgaactgg
    16261 agtcattgct gttggcgctg aataacattg atgccagcca gtttagcaaa taccagttgc
    16321 tgctcgacac catccgaaaa gacctcgcct ggaaagccaa taacacggaa gatcgcctgg
    16381 tgatcttcac cgaaagtatt aaaacgctgg agttcctcga acagcaactg cgagcggatc
    16441 tgaaattgaa agatgaccag atcgccacgc tgcgcggcga tcagggcgat acggtattga
    16501 tggaaaccgt agaagccttt ggtaaaacgc agtcgccgtt gcgtcttctg gtttgttcgg
    16561 atgtggcatc tgaagggatc aacctgcatc accttagcca caaaatgatt cactttgata
    16621 ttccgtggtc gttgatggta ttccagcaac gtaacgggcg tattgaccgc tatgggcaaa
    16681 aacatcagcc ccaaatccgc tatctgctta ccgaggccag cgaaccacag atcaatggcg
    16741 atatgcgcgt actggaagtg ttgatcaaca aagacgaaca ggcgcagaag aacattgggg
    16801 acagttcgga gtttaccggc aaatttaccc aggaagagga agaagagcag gttgcagagt
    16861 tcatgatgca ggatgatggt gccagcctgt ttgatcaact gctgaacagc aacgtctcgg
    16921 aaagcgccga acacgatctg tttggcgaaa tatgcagtgc ggtttccagc gatgcctcaa
    16981 tggtcaccga aacagacacc agcttgtttg ccagcgaaca ggcgtactgc gaaagagcat
    17041 tgggttacct gaaagccagt ggtcaaacta tccagtatga aaccttgcct gataatacct
    17101 tgtcgctggt ggcaccggag gagttacgcc gccgcttcaa ccagctaccg cctgaaattg
    17161 ccccggagaa ctggcagctc tatttaagtc aggataaaac cgtcatcacc gacgcgattg
    17221 cgcgcgctcg cggtgagcaa catgcctggc ccgatgtgca gtatctctgg caaatcaacc
    17281 cggtagtgca gtggctggac gataaaatct cttcggcttt tggtcgtcat caggctccgg
    17341 ttatccggct gccatacctg cttgaacccg atgaagatca cttcattctt tccgggttat
    17401 tcccgaaccg taaatcacat ccgatggtga acccgtggat agtggtgagc tttaaccgtg
    17461 aatcgctaat cggcagtcag cctttcgctg agtttttaca acgtcatccg cagttgagta
    17521 acaagctgac taacagcggc ggtaaagatc gtaatcacca acgccagcag gatttactgg
    17581 aagcggctat tgctcatgcc cgggaggtat tcatccatga tcggaatgcg tttgaaacgc
    17641 acattaacca gcaactgaat gagcatctgc aaaagctgga cgttttgcgt gggcggcagt
    17701 tgagccaact tgagctggat tttgccgata acaaacagca attgtcagtc aagcagagcc
    17761 gtaaagagca gagacaacgc gaaatcgaac acaattttga cagctatatc gaatggattg
    17821 aggacaccat gacgactgaa aaagaaccct acattcaggt aattgctgtt atcaccggag
    17881 cggagggtta atcatggcgc tggtcggtat taataacgaa aacgaatttt actctaacca
    17941 ctatttgggt gaggttttca ccagtgatat ccgcgatgtg ctggaaccct ggatagccca
    18001 ggaaaatgca gcgcgtgaag cggagcgtgc cgctcgtgaa cagggcaaag acgtggagcc
    18061 gggataccgc gctccgtgga accagtttaa cagtctggcg actgagtttt tccgcaaact
    18121 tgccgagcac gaaaaacagc gtcagatccc gcagcgtctg gccgatcaac gtaatcgctg
    18181 gcagccattg ttaaaggcgc tgggctacga aattacgcca cagatccaaa tgctggatga
    18241 cgatacaccg ctgccggtac tggcgcgtta caacagcact gacggtagcc cgtggctgtg
    18301 gattgttgaa gcacacgatc aggaagaagg aacgctggat ccgctggcgc tctccttact
    18361 gaccgcgcaa ttcccggcgg ataccgacaa acataagcac gacagcctgc gcaaaaaagc
    18421 caacggtgaa tatcgcagct ggcaggattt gctctctacg gcggtcttca cccaaaatga
    18481 accgccgcgt tttgtgctgc tgctcggtaa ccgtcagcta ttgttgttgg accgtactaa
    18541 gtgggcgcaa aaccgtctgc tacgttttga ttttgaagag attttaagcc gtcgtgaaac
    18601 ggatacgctg aaagcgactg cggtgttgtt acataaagat tctctgctgc cgggcagtgg
    18661 ggcaccttat cttgactcgc tggatgacaa ttcgcacaaa catgcgtttg gtgtttcgga
    18721 agatctgaaa tatgccctgc gcgaaagcat tgagttgctg ggcaacgaag cgatgcatta
    18781 tctgatcgac cgtggcctgg caaactatac aggtaatcgt gcggtggacc cggatgaact
    18841 gagccgcgaa tgtctgcgtt acatgtaccg cctgctgttc ctgttctaca ttgaagcgcg
    18901 cccggagctg ggttatgcgc caatgaccgc caaaacctat ctgcaaggtt acagcctgga
    18961 aacgttgcgc gatctggaga tgatcccgct gaccagcgaa gaagatcgca acgggcgcta
    19021 cttccacgac agcctgaata tgctgtttaa actggtgcgc gaaggctaca acggcggcgt
    19081 gaaaatgcag agtgacctgg agagcggcga ccggatcacc atccatagtc atcagttcag
    19141 cgtcccgcgt ctggaaagtc atctgtttga tgccaacaac acgcgcattc ttaaccgcgt
    19201 ggtattccgt aacgaaaccc tgcaacagat tatccaggcg atgtcgttaa gccgcccggc
    19261 caaagggcgc tttaaccgcc gcggacgtat ttcttatcgc cagttgggta tcaaccagtt
    19321 gggtgcggtg tatgaggcgc tgctctccta tcgcggattc ttcgccagcg aagatctcta
    19381 cgaggtgaag aaagccgggg aagagtttaa cgagctggag acgggttact tcgtcagtaa
    19441 ggatgagatt agcaaatacc acgaagacga gaaggtctac gagaaagacg gcagtctgcg
    19501 cattcaccgc aaaggcagct ttatctaccg tatggccggg cgcgaccgtg agaaatctgc
    19561 ctcttattac accccggaag tgctgacccg ctcactggtt aaatatgccc tgaaagaact
    19621 gtttaaggag cagattgatc ccattagcga tccgcacgcc aaagctgatg ccatcttaaa
    19681 cctcaccgtg tgcgaaccgg cgatgggcag cgcggcgttc cttaacgaag ccatcaacca
    19741 gctggcggaa gcgtatctgt tccacaagca gcaggcggaa ggtcgccgta ttccgcagga
    19801 tcgttacacc caggagttac agcgggtgaa aatgtacatt gccgacaaca acgttttcgg
    19861 cgtggactta aacccggtgg cggtggaact ggcggaagtg tcgctgtggc tgaacgccat
    19921 tagtggcgat gcgtttgtac cgtggtttgg ttaccagctg cactgcggta actcgctggt
    19981 gggcgcgcgc cgtcaggtgt tcaacaagag cgaactgacc tacaaaaaag ccaaagatcc
    20041 gagctggctt aacagcgagc cggtcgaact ggcgatgaac acgccgcgtg aagagacgca
    20101 gattttccac ttcctgctgc ccgacggcgg tatggctaac tacagcgata aaactgttaa
    20161 gcagcgttat ccggatgact tcaaagcgct ggacagctgg cgcaaagagt ttattaaaag
    20221 ctttgccggg catgagattg ctgatgtgca gcgtatcagc gaaaaggtgg aagcactgtg
    20281 gaacacctat cgccagcaac ttaaagcaga acgtctgaaa accgccgaca gctacccggt
    20341 gtggccggca gaaaacagcg agcagacgcg ttcttcgctg agcagtaaag atgaaacctt
    20401 tagcggtcgt cttgaagata acagcgccta ccagaagctg cgttgggtaa tggactactg
    20461 gtgcgcgctg tggttctggc cgatcgacaa agccgatgag ctaccggatc gcggcacctg
    20521 gttgtttgag attgaaaccc tgcttgacgg gattgtaatc acggaaaaag tcactgaagt
    20581 tgcggagcac accaccggcg atctgtttgc cgaagaaggc ctgctgcgag aagagtcttc
    20641 gctgttttct gttgctggtc gtctgaaaac cgaggtgttg ttccgtcatt tgccgcgtct
    20701 ggcgattgtc gatgccctga gaaagcagca ccgtttcttc cactgggatc tggagttctg
    20761 cgacctgttt gccgagcgcg gcggttttga cctgatgctc ggaaacccgc cgtggctgaa
    20821 agtggaatgg caggaagcgg gcgtgctggg tgattacgag ccggaatttg tgctgcgtaa
    20881 gctgagcgcc tcgaagctgg caacgttgcg tattgatacc tttaaccaga tcccggcgct
    20941 ggaagcggcc tggcgcagcg agtatgaagg ctgtgaaggg atgcaaaact tcctgaatgc
    21001 gcagcagaac tacccggtac tgcgcggggt gcagaccaac ttgtataaat gctttctgcc
    21061 gcaggcctgg cgattagggg cgcagaaagg cgtggcaggt ttcctgcacc cggaagggat
    21121 ttatgatgac ccgaaaggcg ggcaattacg tgcggcggta tatccgaggc tgagggcgca
    21181 ttttcagttt cagaatgagt taaatttgtt tgttgaagtt gatcaccatg cgaagtttag
    21241 cagcaatatt tattctgcta gccctagcac agtgggattt gaacatatat ctaatttgta
    21301 tgctccgcaa actattgatg catgttttga acattctggc agtggggaca ttcccggtct
    21361 caaagacgag attgagagcg agggaaaatt aaaagttgta tggaacacat ctggccaccg
    21421 ttctcgatta ataagtatcg ccactcatga gctagaatta tttgctcgtc tatatgacag
    21481 cgaaggaacg ccagcctggc aggcacgttt gccagcctta catgctaaac aacttgttgc
    21541 tgtactggaa aagtttgcta atcagccgaa tagattaggt gatttgcagg ggcagtattt
    21601 ttcaacggtt atgttcgatg aaacatatgc tcagagggat gggacaattt tacggcagac
    21661 tcaattccct caagattcat cacaatgggt actgtctggc cctcatttct ttgttgggac
    21721 gccgttctac aagactccgc gcgaaaactg tacgcttaac agcgattatg actgcctgga
    21781 cttgctaact ctgcctgacg actatctgcc gcgcactaac tacattccgg catgtgatgc
    21841 acaggagtat gcaaaacgta ctccatgcgt tacatggact gaactggctg aagatgaacc
    21901 gaagaaggta acagattatt atcgcttagc tatcagagcc atgttggctc aatcggggga
    21961 acgtacacta attagtgcta tttatccgcc agaaataagt cacatgaacg cagtacgttc
    22021 ttactgctat agctcacaga atctgttact cgaacattca ggtatgtgtt tttctttacc
    22081 ttttgatttt atttgtaaat ctactggcaa ggcaaactta catcagatgc ttgatggttt
    22141 ctcatacgta ttattcaatc cgagacaaaa ggcattatta tactgcttag tattatcatt
    22201 aaattctgta aatgatgtat atgctggcct ttggcaatcc tgctacaccc cagacttcaa
    22261 cacccagcgt tggagccgcg atctcccgca gctcccccag gatttcttcg ccaaactgac
    22321 cccagagtgg cagcgtaact gcgctttacg ctctgactac agtcgtcgtc aggcgctggt
    22381 ggaaatcgac gtattggtgg cgcaggcgct ggggttaact ctcgaagagc tgcttaccat
    22441 ttatcgcgtt cagttcccgg tgatgcgcca gtacgaagcg gatacctggt acgatcaaaa
    22501 cggtcgcatt atctttaccc caagcaaagg gctggtgggc gttggcttgc ctcgcaccgc
    22561 gcgtaaagct gacctgaaaa acggctttgt ctttaacgtc gacagcccgg agtggaccgg
    22621 cggtgactgc accgatcaag ctatcggttg ggatgatgtc aaacatctta aaaccggtac
    22681 cgtcagcgtc acctttgatg attatacccg cagcgacgaa ggtgagcgcc gtaccgtcac
    22741 ctggcaggct ccgtttatca agccagatcg cgaagatgac tacaaagtgg cctgggcgtt
    22801 ctttgcacaa gataaggaga gcgcctgatg ttgccgtccg ttgtcagtcg gcaggtcgcc
    22861 gacagcgttg ccgccttttt acgggcggcg ttcccgctaa acagcccgct gtttaacggt
    22921 gagaataacg ataatgtctc aatgctggag caatttctgg cccagccgga aacgctgctg
    22981 aaaggaccat acctttccgc ccagttgccg tttcgtaaaa gtgacttacc gctgaatttt
    23041 ttccctaatc tgacgctgcc gttcccgccg cacgcgcatc aggcccgggc atttcagcgt
    23101 ctgggaagcg acgcgccaca gccgacgctg gtcgcaaccg gtaccggctc cggtaaaaca
    23161 gaatgtttta tgttcccgct gctcaaccac tgcgcgggcg cgtcacaagc cggggttaaa
    23221 gcgattatca tctacccgat gaacgccctg gcaaccgatc aggccagccg tttcgccaaa
    23281 accatcgcca gcgatccaca actgcatggc aaggtgaccg tcggcctgtt tgtcggcgac
    23341 agcgaaatcg aacccagcaa aaaaatgtcg gcagaaaagg tcattacctg taagcacacg
    23401 ctgcgcgaaa gcccgccgga catcctgttg accaactaca aaatgctcga ctacctgctg
    23461 atgcgccctg gcgatcagcc gctctggcgt tacaaccagc ccggttcatt gcgttacctg
    23521 gtcgtggatg aattgcatac gtttgatggc gcgcaagggt cggatctggc ctgtctggtg
    23581 cgacgtttga agcatcacat aggtgtggat aaccaacgat tcgcctgcgt cgggacatca
    23641 gctaccgtcg gcgacgaact ggggcaactg ctcgattacg caaaaacaat ctttgaccag
    23701 ccgtttaccg atgacgccgt tattcgcgaa gatcgctaca cggcagcgga gtacttgcag
    23761 aattacgcca ttgcgtacag tcagtatccg gggccggagg tcgcttcggc cctggacccg
    23821 cagagttacg ctaccccggt agcgtatctt aacgggcaaa tccctttgtg gttccccgac
    23881 agcgacttgc aactcccgga cgatctggag agcgacgacg ggcgtgaaaa gcgtattgcg
    23941 ctggggtcac tgttacgccg ccacagcgtg atgcatgtct tactccatga tctgcaaggc
    24001 gggatcctct ctgaacgaga gtgtctggaa aatctccagg tcatgctggc agaaagcgcc
    24061 gaccatgccc gacgcgtgct gcaaagcatt ctggcactga tcgcgcaggc acgtctcgaa
    24121 gtaccggaaa cggaacacga tcggcaaaaa cgtctccagg cgaagaaagc gcgtccggtg
    24181 ctgccgtttg tgcaactgcg ctcccagttg tggcttcgtg agatgcgtcg tctggtcgcc
    24241 agcgtggcga aaacgccccg actggtgttt gccgatgatg tggcggtgga agatcgcgaa
    24301 cactatttgc cggttatcca ctgccgtgac tgccacgcaa ctggctgggc cagcctgcgt
    24361 catgggcaaa gcgttcagtt ggaaacagac ctcgacggca tttatcgcca gttctttgaa
    24421 aaagggcgct ccatcgtcct tgctttcccg gataacaacg aaaaagcggt cagtggcgca
    24481 caccagaaac tgtgtcctac ctgtctgaaa ctgaaccgac agtccagcgt ccagtgtgga
    24541 cattgcggtc atacggaact gttgcaggta ttgatcccgg atatgctcaa agagcgtaag
    24601 gacgagctgg agtttgctaa tgaatgcccg tactgcaaca gcaaacaggg gatttcgatt
    24661 ctggggtcac gggctgccag tctgtcggcg gtgatgattc accagcttta tggcagccag
    24721 ttcaacgaac ataaaaagct gattacgttt tctgactcgg tacaagacgc cgcgcatcgt
    24781 gccagcttct ttactgcccg tacctggccg ctgatgattc gtgggcaaat cgccagtgtt
    24841 cttagcgaaa aagagacatt accgctggat gcgttcgcgg cgcgtgtttg ccaacaaacg
    24901 cgtgaacgtt caccggacga tgctcatttt gcagcaaacg tcatcgcacc aaatatgcag
    24961 tggctgaatg attatcagac gctgaaaaat gaaggcaaac tggcggagaa ctccgatctg
    25021 ccggagctgg tttccctgcg cctcgactgg gaggtgtaca gcgaatttac cctgcgcgcg
    25081 cgtatcggac gtacgctgga agccaccggt tatgccgttg ccggagtgga taaagatcgc
    25141 ttcaatccgg caattaccgc cctgcatcaa cagctttgtg aagagctggg tgatgagatg
    25201 tctggtgtga gcgtcgagca ggtagcgcac ctggcgctgg gggttatctg gtatcaacgc
    25261 caggccgggg ccgttatgca tccggcgttc gaaagttatc gccgtgatgg cacgaccttt
    25321 atgatgacgc aaaaagagcg tacctcgcgc tatatgcctt ctatcggtcc gaaaacgcgt
    25381 aaaccggctt atgcgtcgtt tgaaaaaatt aatggtttcc atcgcctgat tggcgcgaag
    25441 tctaacccca gttggtatca gcattggatc aatcgcacgc tgagtaacgg caataatctg
    25501 tttatcagta gcgttgcgga aacagtgtta cgacgcctgt ttacggcgtt gaaaatagcc
    25561 ggagtagtaa aagattttga caccaaaggg cgtgaagcct ggggactggt gccttcggcg
    25621 ctggtggtga gccgtgacgt cgttcagctt aattgctcat gttgccgtga ggttgtccgg
    25681 gctccggccg atcaaggctg gcagtggcgt aatgcgcctt gtctctcttt acgttgtgaa
    25741 ggtcgttacc aggaagtcga caatacggcg acctggcgtt gggagaacat ggacatcgcg
    25801 cgcgtacagg gcgcagaaca cacggggctg cttagccgtg aagatcgcga agtcaccgaa
    25861 aaatcgttct accgcggcaa ccaaccgtgg aatatcaacc tgttgtcggc aacaccgacg
    25921 ctggagatgg gtattgacgt tggggatctt tccaccgttt tactctgttc tgtcccacct
    25981 gctcaggcca actacttgca gcgtattggt cgcgccggtc gtaaagatgg taacgcgtta
    26041 aacattaccg tggcagaagg gaacccgcac gatcagtttt tctttgaaga accactggag
    26101 atgatgcagg gccaggtgca ggctcctggc gtcttcctta atgcaacggc tattctcgaa
    26161 cgccagctgg cagctttttg tatggataac tgggtgaaaa ccggtgtacc agaatccgct
    26221 atctgtaaaa acgtcaaaca gatgctggat gagctggagt tcgggcgtaa atcgggtttt
    26281 ccgtataact tactgcgata tatcgaacag catcatgcgg agattgctgc gcaatttacg
    26341 gcaatcttcc ccgatcttgc cgcagaaact cgccaacagc tgttgtctta tcttcagggc
    26401 gcgccaggac aacgctcgct ggtgcagcgt atcgaagaag cgttaaaact gctggtggaa
    26461 gatcgcaaat ctttgcgctc acgcatcgat aaactcaagc gcagtatcga taaactggag
    26521 aacgcgccgc gcgatcaaaa ctttgatagc gatatgcgcg agttaacttc ggaacggcag
    26581 gcgctgatgg cgctggtcaa tcaaatcaac aataagcaaa cgctgaattt cctcacggat
    26641 gaaggtctgc tgcctaacta cgccttcccg gaagccggta ttacgttacg ttctgtgctc
    26701 tggcggcgta aagaaggcgg ggaagcacgt gaatatcaaa acaccacttt cgaatatgaa
    26761 cgcccggcat caacggcgct ggctgagctg gctccgctga ataatttcta tgccggtggg
    26821 cataaagttg aaattgagca gatagacctg aaggtctctg agccggagaa ctggcgtatc
    26881 tgtagccact gtaactacag cgaaaatatc gaccagacgg gcgaccagca taaatattgc
    26941 ccgaaatgtg gcacaccggg gtgggccgat gccggacaaa aaaccacgct gctgaaactt
    27001 cgccaggttt atgcccgcgc cagcgcacgt gatagccaga tcagcgacga gagcgacagc
    27061 cgtgaacctg ctttcttcca gcgtcagcta ttggttagtt tcgaaaaaga agatgtatcg
    27121 gcggcatacg cgattgagga aggcgaagtt ccgtttggtt ttgaattttt aagcaaagtc
    27181 accctgcggg atatcaactt cggcaaaatg gcggatgatg ccaatgagtt gatgattgcc
    27241 ggagaagcga agaagcgaac cgggtttaaa gtttgcctcg ggtgcggcat ggtgcagcgt
    27301 cctcgcgatc atgaaccacg tcatgatttg agctgcaaat atcgcacgga accggaaaag
    27361 gcgaagttcg aagattatct ctacctctat cgccagctgg agtcagaagc gttgcgtatt
    27421 ctgctcccgg taagcagtta cagtaatgac cgtgtcgtgg aggcttctct tggggcggcg
    27481 atccagctcg gtcttaaaca ttacttcaag gggaatgtcg atcacctgaa aggcgttgtg
    27541 taccgcgagc cggaaaatga aggcgaatcc tggcggcagt atctggtgat ttacgatacc
    27601 gtcccggggg ggactggttc tcttaaagaa ctgatgcgca ctcccgacaa tttgctgaaa
    27661 ttacttgaac tggcctatca ggcaatagtg gaatgcagtt gcaacaatga tacgcataaa
    27721 gatggttgtt atcgttgcgt ttacgcttac cgtgaccgtg ggcgcatgaa gtatgtctcc
    27781 cgcgatcagg cgcgtctctt gctggcgaaa attttgcagg caagagattc gatacgggta
    27841 attgattcca tcaaaaacat ttccctcgaa gccatgatgg gcagtgagct ggaaaaacgg
    27901 tttattagct gcctgcaaga aaataaaaac ctgattgtca gtcgcagtta cgcgcatcag
    27961 ggcgctgggt ggattattaa cacccgcagc gaaccgatga tgagctggca tcttaaagct
    28021 caggtggatt taggcgctaa agaggcggtg gggatccctt gtcgcccgga ttacgtgcta
    28081 tatccgctta tgcagtctga tgccattaaa ccggtggcta ttttccttga tggctttgcg
    28141 ttccacaaaa atagcgtcgc agaggatgtg caaaaacgcc aggcgattcg ggatagcggt
    28201 aatttctggg tgtggacagt cacgtgggcc gatctcaaag agccagggct taaacatgtt
    28261 caggatgtcc tgggacttgg gcataacccg gatatgaaac agcctaagtt ttataacctg
    28321 tttcatgata ccaactttgc ggcccttgaa gcgtcgttcc gggaacgcaa cagttttgca
    28381 ctgctgctgg attatctcgc cgatccgggc ggaagaacgc agttatggca gagaatggct
    28441 gctgcacatg cctgggtgtg gttggatgtg aagaagtcac aggacgcggg aacgaaacaa
    28501 aagtatgcct atgaaatgca ggaaaacgcg ccagcttggc ggctagcaga gcttttgcca
    28561 gacgaaccgt ttgtctttgg tggtttactc gatagctgta actcatcgca gcaatttatt
    28621 gagctggctt cggtgttacc acaacaagcc attaaaccga cgaccagcgt cgcacaaatg
    28681 cgcagctggc tgcgtttgca tatctgtttt gacgatcgct atacgcagga tgacggttat
    28741 gaagcgggac tcaatggctt ctggcgactg gtaaacctgt tacagttcct gccggatatg
    28801 acctttacca gccgtaaagc cgtgcattta ccgcaggaaa gcgttgacac taaagtttcg
    28861 gcaacgccag aggcagtacc agagactgat gagagttggg ctgagattat tgagtttggc
    28921 ttattaagcg ctgaagagat cgcgttgctt cagtctcatt ctctctctgc gccgactctc
    28981 ggctacgaat tgcaaaatga tgaaggtgaa attattgcgg aagccgatct cgcctggcca
    29041 tcacaaaaat gggcattgat tattgataat caagaattta ttccattatt cacaaccagg
    29101 ggttggcacg ttgcattcgg gccaattgac gaaaatacat tgcaacatct gtccggaggt
    29161 gacaaatgac ggtgcaaacg cccaaggtgg cactttctga tggttttctg ggcgcattcg
    29221 cgcggatccc aaaggcacag caaaaaaagg tgcaggaatt catctccaaa ttccgtcagg
    29281 acccgaccag taatggactt aactatgaaa agatccacga tgcgcgcagt aaaaatgtcc
    29341 attcggtgcg aatcgatcaa acttatcgcg gcattgttct gaaacctgaa cagggtgccc
    29401 tgtatatgct gatgtgggtg gataaacacg acgaagcata tgactgggcg cggcggcatg
    29461 attgttcaat tcacccggtt accggcgcga ttcaggtcat tgatattagc tatatcaaac
    29521 cggcagcgga aacagtggtt gataaaccga aactcttcgc tgcttacagc gcggaacaga
    29581 ttctggctct gggtgtgccg cctgtcttta ttgatcaggt gatggcgctt accgatgaag
    29641 ccgggcttaa tcaacttgaa agcataatgc cagcagaagc ctgggaacct ctgcactggc
    29701 tggcagaagg gctggattac caggaagtgc tggaagagtt taacgatcat cgtgatgagc
    29761 ctgttgatac caacgacttt atggaggcga ttgaacgaag taaacgtcgc ttccatgtcg
    29821 ttgaaaatga acaggagctg ttgcagatgc ttaatgcgcc tttagaaaaa tggcgtgtat
    29881 ttctgcatcc gagccagcga aaattggtgg agtcgcccgc taatggtccg gtacgagtac
    29941 tgggcggtgc aggtacaggg aaaacggttg ttgcgatgca ccgtgcgcgg tggttatctc
    30001 agcgtctggc agataaaccc ggtaagaaag tacttttcac gacctttacg cgtaacctgg
    30061 cggcagatat tcgcgctaat ctccagcggt tgtgtacgcg agaagagatg gcgcgtattg
    30121 acgtcgttaa cattgatgcc tggatgagcg accaactaaa acgccacggt tatgatttcc
    30181 gcgtggtata cgacagcgat gaagggcgac gcaaatgctg gaactatgcc ttgcagcagg
    30241 cacctgccga actggggcta cctgataatt tctatgctga agagtggcaa cgcgttgtcc
    30301 agccaaatgc ggtttacacc cgggaggaat acctcaaagt cagccgggtt ggacgtggta
    30361 cagcgttatc ccgtattcaa cgggcgaaaa tctggccagt ctttgaagaa ttcagagcac
    30421 aaatggcgcg tgccaaactg cgcgaaatgg gcgatgccat gcatgaggct attgttctgt
    30481 ttaaagaaaa gcaggtccaa ctaccttata gcagtattgt ggtcgatgaa gctcaagata
    30541 tcggtgcccc ggcatttact cttattcgca gcctggttcc cgagtcgccc aacgatctgt
    30601 ttattgtcgg ggatgggcat cagcgtattt atcgcaataa ggtcgttctt ggtcagtgtg
    30661 gtattaacgt ccgtggtcgt cgcagtaaaa aattgaagat taactatcgg accacagaag
    30721 agacgcgtca atttgctgtt gggctgttaa ctggtgtgaa ggttgataac ctcgatggcg
    30781 aagcggatac cagtaatgat tatttgtcat tactgcacgg ggaaaagccc atgattacgc
    30841 atgcggcaga tttcaaagaa gaggctgcaa cgctcgtcaa gcaaattcag gcattgctgg
    30901 cgaatcaggt tcgttctcag gatatctgca ttactgcgcg tactaaacat gcttgtgacc
    30961 gctacgcatc agcactgaat gacgccggaa ttgagacatt taatttaggc aatgatagcg
    31021 gtgacagcga tgcacgccct ggcgtgcggg ttgcaacgat gcaccgtatt aaagggcttg
    31081 agttccagta tgtgttcctg gtggggatta acgaaggcgt tgtgccggaa ataaaagcat
    31141 tagcatccga tgatcccgtt gaacagcgtg atgcattatt taatgagcgc gcgttactgc
    31201 atgtggctgc aacccgtgcc gtaaaagggc tgtttgtttc atcatctggt gtccctagtc
    31261 cgctattagt ggctgactga taatatgtcc aaaacaggcc aggagccgaa acgctcctgg
    31321 tctatataac cgttctggca actggcgata tttatattgc ttagctccca acagccagac
    31381 aatatcaact tagaaattat attcgcttat gcggtttgca tattgcacag tacaacctgt
    31441 tttctctata tctaacgtaa cgtcaggatg tggatgattt ttggatgggg tgacacactg
    31501 gacaaatata gctggcacga aaaggctggc atttccagct gaccaatagt ctctggaacc
    31561 atgatgatga taaaataaat atcttaagtt ttaaacgtat gatgccagga ttaccagaat
    31621 tataaacgaa tatattagtt cttttctatt tgttcaggag gggtataaaa attgcaattg
    31681 ttaatagtat cgtcaggacg ataaaaagat aatcatgaca aaggcaattg aactaaaatg
    31741 attgacattt tacaggt
//
LOCUS       DAZKIP010000046        31590 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZKIP010000046 DAZKIP010000000
VERSION     DAZKIP010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.046"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            247..1815
                     /gene="cydA"
                     /locus_tag="ACHOUK_002329"
     CDS             247..1815
                     /gene="cydA"
                     /locus_tag="ACHOUK_002329"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFP9897777.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            1831..2970
                     /gene="cydB"
                     /locus_tag="ACHOUK_002330"
     CDS             1831..2970
                     /gene="cydB"
                     /locus_tag="ACHOUK_002330"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFP9897778.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            2985..3098
                     /gene="cydX"
                     /locus_tag="ACHOUK_002331"
     CDS             2985..3098
                     /gene="cydX"
                     /locus_tag="ACHOUK_002331"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFP9897779.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            3098..3391
                     /gene="ybgE"
                     /locus_tag="ACHOUK_002332"
     CDS             3098..3391
                     /gene="ybgE"
                     /locus_tag="ACHOUK_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFP9897780.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            3541..3945
                     /gene="ybgC"
                     /locus_tag="ACHOUK_002333"
     CDS             3541..3945
                     /gene="ybgC"
                     /locus_tag="ACHOUK_002333"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFP9897781.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            3942..4634
                     /gene="tolQ"
                     /locus_tag="ACHOUK_002334"
     CDS             3942..4634
                     /gene="tolQ"
                     /locus_tag="ACHOUK_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFP9897782.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            4638..5066
                     /gene="tolR"
                     /locus_tag="ACHOUK_002335"
     CDS             4638..5066
                     /gene="tolR"
                     /locus_tag="ACHOUK_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFP9897783.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            5131..6345
                     /gene="tolA"
                     /locus_tag="ACHOUK_002336"
     CDS             5131..6345
                     /gene="tolA"
                     /locus_tag="ACHOUK_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFP9897784.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADDKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAAAEKAAA
                     DKKAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAAAAKAAAEKAAAAKAAAE
                     ADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAGQIKSAIESK
                     FYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKPPSQAVY
                     EVFKNAPLDFKP"
     gene            6478..7770
                     /gene="tolB"
                     /locus_tag="ACHOUK_002337"
     CDS             6478..7770
                     /gene="tolB"
                     /locus_tag="ACHOUK_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFP9897785.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            7805..8326
                     /gene="pal"
                     /locus_tag="ACHOUK_002338"
     CDS             7805..8326
                     /gene="pal"
                     /locus_tag="ACHOUK_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFP9897786.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            8336..9127
                     /gene="cpoB"
                     /locus_tag="ACHOUK_002339"
     CDS             8336..9127
                     /gene="cpoB"
                     /locus_tag="ACHOUK_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFP9897787.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVIQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSGDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            9292..9367
                     /locus_tag="ACHOUK_002340"
     tRNA            9292..9367
                     /locus_tag="ACHOUK_002340"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9325..9327,aa:Lys,seq:ttt)
     gene            9403..9478
                     /locus_tag="ACHOUK_002341"
     tRNA            9403..9478
                     /locus_tag="ACHOUK_002341"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9436..9438,aa:Val,seq:tac)
     gene            9481..9556
                     /locus_tag="ACHOUK_002342"
     tRNA            9481..9556
                     /locus_tag="ACHOUK_002342"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9514..9516,aa:Lys,seq:ttt)
     gene            9608..9683
                     /locus_tag="ACHOUK_002343"
     tRNA            9608..9683
                     /locus_tag="ACHOUK_002343"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9641..9643,aa:Val,seq:tac)
     gene            9687..9762
                     /locus_tag="ACHOUK_002344"
     tRNA            9687..9762
                     /locus_tag="ACHOUK_002344"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9720..9722,aa:Lys,seq:ttt)
     gene            9909..9984
                     /locus_tag="ACHOUK_002345"
     tRNA            9909..9984
                     /locus_tag="ACHOUK_002345"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9942..9944,aa:Lys,seq:ttt)
     gene            10262..11305
                     /gene="nadA"
                     /locus_tag="ACHOUK_002346"
     CDS             10262..11305
                     /gene="nadA"
                     /locus_tag="ACHOUK_002346"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFP9897788.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKKTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNYEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            11343..12062
                     /gene="pnuC"
                     /locus_tag="ACHOUK_002347"
     CDS             11343..12062
                     /gene="pnuC"
                     /locus_tag="ACHOUK_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFP9897789.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVIIMQALGLQVAMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(12059..13000)
                     /gene="zitB"
                     /locus_tag="ACHOUK_002348"
     CDS             complement(12059..13000)
                     /gene="zitB"
                     /locus_tag="ACHOUK_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFP9897790.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(13114..13494)
                     /gene="ybgS"
                     /locus_tag="ACHOUK_002349"
     CDS             complement(13114..13494)
                     /gene="ybgS"
                     /locus_tag="ACHOUK_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFP9897791.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHPDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            13810..14862
                     /gene="aroG"
                     /locus_tag="ACHOUK_002350"
     CDS             13810..14862
                     /gene="aroG"
                     /locus_tag="ACHOUK_002350"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFP9897792.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            complement(15028..15780)
                     /gene="gpmA"
                     /locus_tag="ACHOUK_002351"
     CDS             complement(15028..15780)
                     /gene="gpmA"
                     /locus_tag="ACHOUK_002351"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFP9897793.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(15982..17022)
                     /gene="galM"
                     /locus_tag="ACHOUK_002352"
     CDS             complement(15982..17022)
                     /gene="galM"
                     /locus_tag="ACHOUK_002352"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFP9897794.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGKQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNYLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLHPGEE
                     YSSLTEYQFIAE"
     gene            complement(17016..18164)
                     /gene="galK"
                     /locus_tag="ACHOUK_002353"
     CDS             complement(17016..18164)
                     /gene="galK"
                     /locus_tag="ACHOUK_002353"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFP9897795.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            complement(18168..19214)
                     /gene="galT"
                     /locus_tag="ACHOUK_002354"
     CDS             complement(18168..19214)
                     /gene="galT"
                     /locus_tag="ACHOUK_002354"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFP9897796.1"
                     /translation="MTQFNPVDHPHRRYNQLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            complement(19224..20240)
                     /gene="galE"
                     /locus_tag="ACHOUK_002355"
     CDS             complement(19224..20240)
                     /gene="galE"
                     /locus_tag="ACHOUK_002355"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFP9897797.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            complement(20502..21974)
                     /gene="modF"
                     /locus_tag="ACHOUK_002356"
     CDS             complement(20502..21974)
                     /gene="modF"
                     /locus_tag="ACHOUK_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFP9897798.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITPLLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYHYALTKIN"
     gene            complement(22042..22830)
                     /gene="modE"
                     /locus_tag="ACHOUK_002357"
     CDS             complement(22042..22830)
                     /gene="modE"
                     /locus_tag="ACHOUK_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFP9897799.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            22959..23108
                     /gene="acrZ"
                     /locus_tag="ACHOUK_002358"
     CDS             22959..23108
                     /gene="acrZ"
                     /locus_tag="ACHOUK_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFP9897800.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            23275..24048
                     /gene="modA"
                     /locus_tag="ACHOUK_002359"
     CDS             23275..24048
                     /gene="modA"
                     /locus_tag="ACHOUK_002359"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9897801.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            24048..24737
                     /gene="modB"
                     /locus_tag="ACHOUK_002360"
     CDS             24048..24737
                     /gene="modB"
                     /locus_tag="ACHOUK_002360"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFP9897802.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            24740..25798
                     /gene="modC"
                     /locus_tag="ACHOUK_002361"
     CDS             24740..25798
                     /gene="modC"
                     /locus_tag="ACHOUK_002361"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFP9897803.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(25799..26617)
                     /gene="ybhA"
                     /locus_tag="ACHOUK_002362"
     CDS             complement(25799..26617)
                     /gene="ybhA"
                     /locus_tag="ACHOUK_002362"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFP9897804.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDEMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            26772..27767
                     /gene="pgl"
                     /locus_tag="ACHOUK_002363"
     CDS             26772..27767
                     /gene="pgl"
                     /locus_tag="ACHOUK_002363"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFP9897805.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNIDHRGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(27808..28761)
                     /gene="ybhD"
                     /locus_tag="ACHOUK_002364"
     CDS             complement(27808..28761)
                     /gene="ybhD"
                     /locus_tag="ACHOUK_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9897806.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            28945..29997
                     /gene="ybhH"
                     /locus_tag="ACHOUK_002365"
     CDS             28945..29997
                     /gene="ybhH"
                     /locus_tag="ACHOUK_002365"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFP9897807.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEKRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGGLDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            30073..31506
                     /gene="ybhI"
                     /locus_tag="ACHOUK_002366"
     CDS             30073..31506
                     /gene="ybhI"
                     /locus_tag="ACHOUK_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFP9897808.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESSVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
BASE COUNT         7555 a         7708 c         8339 g         7988 t
ORIGIN      
        1 tgtcgaaaaa tgcaaatttg ctcaataaaa acctgtttat tgtaaggatt ttgcggcgta
       61 atataattac gggatcaatt tggtttattc attaacatat ttgtaacttt tttatcattg
      121 tcttttcctc ttttgatgaa atggcaacga tagaggcgag caaataattt tgtattgggg
      181 catgcgtgtg accctttcta acggggttca ctctcggagt cttcatgcga tgagcaagga
      241 gtcatgatgt tagatatagt cgaactgtcg cgcttacagt ttgccttgac cgcgatgtac
      301 cacttccttt ttgtgccact gacgctcggt atggcgttcc tgctggccat tatggaaacg
      361 gtctacgtcc tttccggcaa acagatttat aaagatatga ccaagttctg gggcaagttg
      421 tttggtatca acttcgctct gggtgtggct accggtctga ccatggagtt ccagttcggg
      481 actaactggt cttactattc ccactatgta ggggatatct tcggtgcgcc gctggcaatc
      541 gaaggtctga tggccttctt cctcgaatcc acctttgtag gtctgttctt cttcggttgg
      601 gatcgtctgg gtaaagttca gcatatgtgt gtcacctggc tggtggcgct cggttcaaac
      661 ctgtccgcac tgtggattct ggttgcgaac ggctggatgc aaaacccaat cgcgtccgat
      721 ttcaactttg aaactatgcg tatggagatg gtgagcttct ccgagctggt gcttaacccg
      781 gttgctcagg tgaaattcgt tcacactgta gcgtctggtt atgtgactgg cgcgatgttc
      841 atcctcggta tcagcgcatg gtatatgctg aaaggtcgtg acttcgcctt cgctaaacgc
      901 tcctttgcta tcgctgccag cttcggtatg gctgccgttc tgtctgttat tgttctgggt
      961 gatgaatccg gctacgaaat gggcgacgtg cagaaaacca aactggctgc tattgaagct
     1021 gagtgggaaa cgcaacctgc gcctgctgcc tttactctgt tcggcattcc tgatcaggaa
     1081 gaggagacga acaaatttgc gatccagatc ccttacgcgc tgggcataat tgcaacgcgt
     1141 tccgtggata ctccagttat cggcctgaaa gaactgatgg tgcagcatga agaacgcatt
     1201 cgtaacggga tgaaggcgta ctctctgctc gaacaactgc gttctggttc taccgaccag
     1261 gcggttcgtg accagttcaa tagcatgaag aaggacctcg gttacggtct gctgctgaaa
     1321 cgctatacgc caaacgtggc tgatgcgact gaagcgcaga ttcaacaggc aaccaaagac
     1381 tccattccgc gtgtagcgcc gctgtacttc gcgttccgta tcatggtggc gtgtggcttc
     1441 ctgctgctgg caatcatcgc gctctctttc tggagtgtca tccgcaaccg cattggcgag
     1501 aaaaaatggc tcctgcgcgc cgcgctgtac ggtattccgc tgccgtggat tgctgtagaa
     1561 gcgggctggt tcgtggctga atatggccgc caaccgtggg ctatcggtga agtgctgccg
     1621 acagctgtgg cgaactcgtc actgaccgca ggcgatctca tcttctcaat ggtgctgatt
     1681 tgcggcctgt ataccctgtt cctggtggca gaattgttct taatgttcaa gtttgcacgc
     1741 ctcggcccaa gcagcctgaa aaccggtcgc tatcactttg agcagtcttc cacgactact
     1801 cagccggcac gctaagacag gagtcgtcaa atgatcgatt atgaagtatt gcgttttatc
     1861 tggtggctgc tggttggcgt tctgctgatt ggttttgcag tcactgatgg tttcgacatg
     1921 ggggtgggca tgctcacccg tttcctcggt cgtaacgaca ccgagcgtcg aattatgatt
     1981 aactccatcg caccacactg ggacggtaac caggtttggc tgatcaccgc aggcggcgca
     2041 ctgtttgctg cctggccgat ggtctatgcc gctgcgttct ccggcttcta tgtggcgatg
     2101 atcctcgtgc tggcgtcttt gttcttccgt ccggtcggtt ttgactaccg ctccaagatt
     2161 gaagaaaccc gctggcgtaa catgtgggac tggggcatct tcattggtag cttcgttccg
     2221 ccgttggtga ttggtgtagc gttcggtaac ctgttgcagg gcgtaccgtt caacgttgat
     2281 gaatatctgc gtctgtacta caccggtaac ttcttccagc tgcttaaccc gttcggcctg
     2341 ctggcaggcg tggtgagcgt agggatgatc attactcagg gcgcaaccta tctgcaaatg
     2401 cgtaccgtgg gcgaactgca cctgcgtacc cgtgcaacgg ctcaggtggc ggcactggtg
     2461 acactggtct gtttcgcact ggctggcgta tgggtgatgt acggtatcga tggttatgtc
     2521 gtgaaatcga caatggacca ttacgcagcc tctaacccgc tgaataaaga agtggttcgt
     2581 gaagctggcg catggctggt taacttcaac aacacgccaa ttctgtgggc tattccggca
     2641 ctgggtgtgg ttctgccgct gctgaccatc ctgactgcac gtatggataa agccgcgtgg
     2701 gcgtttgtgt tctcctccct gacgctggcc tgcatcatcc tgactgccgg tatcgcaatg
     2761 ttcccgtttg tgatgccgtc cagcaccatg atgaacgcaa gtctgacaat gtgggatgca
     2821 acttccagcc agctgacact taacgtcatg acctgggttg cggtggttct ggtaccgatc
     2881 attctgctct acaccgcctg gtgttactgg aaaatgttcg gtcgtatcac caaagaagat
     2941 attgaacgta acacccactc tctgtactaa gtaaggagct aaaaatgtgg tatttcgcat
     3001 ggattctggg aacgcttctt gcctgttcgt ttggggtaat caccgcgctg gcgcttgagc
     3061 acgtcgaatc aggcaaagcc ggtcaagaag acatctgatg agtaagatta tcgcaacctt
     3121 gtatgcggta atggacaagc gccccctgcg ggcgctttcc ttcgtgatgg cgcttctgtt
     3181 agcaggatgt atgttttggg acccatctcg tttcgccgcg aagaccagtg aactggaaat
     3241 ctggcatggt ttattgctga tgtgggccgt ctgtgctggt gtgattcacg gcgtgggctt
     3301 tcgtccgcag aaggttcttt ggcaagggat tttttgccca ttgcttgccg atattgttct
     3361 cattgtcggg ctgattttct tcttctttta aatcagaatt ctctttaaaa aattatgggc
     3421 cgtgccaggc ccataaattt ttacgctccc ttaacttgcc ctcattccca aacctcaatc
     3481 gcgcgcgtat agtagcagcg tttaaaagct ctaacttttg ttgcattacc gggatgtaaa
     3541 gtgaatacaa cgctgtttcg atggccggtt cgcgtctact atgaagatac cgatgccggt
     3601 ggtgtggtgt accacgccag ttacgtcgct ttttatgaaa gagcacgcac agagatgctg
     3661 cgtcatcatc acttcagtca acaggcgctg atggctgaac gcgttgcctt tgtggtacgt
     3721 aaaatgacgg tggaatatta cgcacctgcg cggctcgacg atatgctcga aatacagact
     3781 gaaataacat caatgcgtgg cacctctttg gttttcacgc aacgtattgt caacgccgag
     3841 aatactttgc tgaatgaagc agaggttctg gttgtttgcg ttgacccact caaaatgaag
     3901 cctcgtgcgc ttcccaagtc tattgtcgcg gagtttaagc agtgactgac atgaatatcc
     3961 ttgatttgtt cctgaaggct agccttctgg ttaaacttat catgttgatt ttgattggtt
     4021 tttcaatcgc atcctgggcc attattatcc agcggacccg tattcttaac gcagcggcgc
     4081 gcgaagccga agcgtttgaa gataaattct ggtctggaat cgaactctct cgcctctatc
     4141 aagagagcca ggggaaacgg gataatctga ctggttcgga acaaatcttt tacagcgggt
     4201 tcaaagaatt tgtgcgcctg catcgtgcca atagccatgc gccggaagcc gtagtggaag
     4261 gggcgtcgcg tgcgatgcgt atttccatga accgcgaact tgaaaatctg gaaacgcaca
     4321 ttccgttcct cggtacggtt ggctccatca gcccgtatat tggtctgttt ggtacggtct
     4381 gggggatcat gcacgccttt atcgccctcg gggcggtaaa acaagcaaca ctgcaaatgg
     4441 ttgcgcccgg tatcgcagaa gcgttgattg cgacggcaat tggtctgttc gccgctattc
     4501 cggcggtaat ggcttataac cgtctcaacc aacgtgtaaa caaactggaa ctgaattacg
     4561 acaactttat ggaagagttt accgcgattc tgcaccgcca ggcgtttacc gttagcgaga
     4621 gcaacaaggg gtaagccatg gccagagcgc gtggacgagg tcgtcgcgat ctcaagtccg
     4681 aaatcaacat tgtaccgttg ctggacgtac tgctggtgct gttgctgatc tttatggcga
     4741 cagcgcccat catcacccag agcgtggagg tcgatttgcc agacgctact gaatcacagg
     4801 cggtgagcag taacgataat ccgccagtga ttgttgaagt gtctggtatt ggtcagtaca
     4861 ccgtggtggt tgagaaagat cgcctggagc gtttaccacc agagcaggtg gtggcggaag
     4921 tgtccagccg tttcaaggcc aacccgaaaa cggtctttct gatcggtggc gcaaaagatg
     4981 tgccttacga tgaaataatt aaagcactga acttgttaca tagtgcgggt gtgaaatcgg
     5041 ttggtttaat gacgcagcct atctaaacat ctgcgtttcc cttgcttgaa agagagcggg
     5101 taacaggcga acagtttttg gaaaccgaga gtgtcaaagg caaccgaaca aaacgacaag
     5161 ctcaagcggg cgataattat ttcagcagtg ctgcatgtca tcttatttgc ggcgctgatc
     5221 tggagttcgt tcgatgagaa tatagaagct tcagctggag gcggcggtgg ttcgtccatc
     5281 gacgctgtca tggttgattc aggtgcggta gttgagcagt acaaacgcat gcaaagccag
     5341 gaatcaagcg cgaagcgttc tgatgagcag cgcaagatga aggaacagca ggctgctgaa
     5401 gaactgcgtg agaaacaagc ggctgaacag gaacgcctga agcaacttga gaaagagcgg
     5461 ttagctgctc aggaacagaa aaagcaggct gaagaagccg caaaacaggc cgagttaaag
     5521 cagaagcaag cggaagaggc ggcagcgaaa gcggcggcag atgctaaagc gaaggccgaa
     5581 gcggatgata aagctgcgga agaagcagcg aagaaagcgg ctgcagacgc gaagaaaaaa
     5641 gcagaagcag aagccgccaa agccgcagcc gaagcgcaga aaaaagccga ggcagcagct
     5701 gcggcgctga agaagaaagc ggaagcggca gaagcagctg cagctgaagc aagaaagaaa
     5761 gcggcagcag agaaagctgc agccgacaaa aaagcagcag agaaagctgc agccgacaaa
     5821 aaagcagcag aaaaagcggc tgctgaaaag gcagcagctg ataagaaagc agcggcagaa
     5881 aaagccgccg cagacaaaaa agcggcagct gcaaaagcag cagctgaaaa agccgctgca
     5941 gcaaaagctg ccgcggaggc agatgatatt ttcggtgagc taagctctgg taagaatgca
     6001 ccgaaaacgg ggggaggggc gaaagggaac aatgcttcgc ctgccgggag tggtaatact
     6061 aaaaacaatg gcgcatcagg ggccgatatc aataactatg ccgggcagat taaatctgct
     6121 atcgaaagta agttctatga cgcatcgtcc tatgcaggca aaacctgtac gctgcgcata
     6181 aaactggcac ccgatggtat gttactggat atcaaacctg aaggtggcga tcccgcactt
     6241 tgtcaggctg cgttggcagc agctaaactt gcgaagatcc cgaaaccacc aagccaggca
     6301 gtatatgaag tgttcaaaaa cgcgccattg gacttcaaac cgtaatcgcg atgttgactg
     6361 ttccgacggt caacatcagg caccggttgc cacggggttc tggtagtttt gtgtatttta
     6421 gtttgttaac attctgctaa attatcgtgg gccatcggtc cagataaggg agatatgatg
     6481 aagcaggcat tacgagtagc atttggtttt ctcatactgt gggcatcagt tctgcatgct
     6541 gaagtccgca ttgtgatcga cagcggtgta gattccggtc gtcctattgg agttgttcct
     6601 ttccagtggg cgggccctgg tgcggcacct gaagatattg gcggcatcgt tgctgccgac
     6661 ttgcgtaaca gcggtaaatt taatccgtta gatcgcgctc gtctgccaca gcagccgggt
     6721 agtgcgcagg aagtacaacc agctgcatgg tccgcgctgg gtattgacgc tgtagttgtc
     6781 ggtcaggtca ctccgaatcc ggatggttct tacaatgttg cttatcaact tgttgacact
     6841 ggcggcgcac cgggtactgt acttgctcag aactcgtaca aagtgaacaa gcagtggctg
     6901 cgttatgctg gtcataccgc cagtgatgaa gtgtttgaaa aactgaccgg tattaaaggt
     6961 gcgttccgta cccgtattgc ctacgttgtt cagaccaacg gcggtcagtt cccgtatgaa
     7021 ctgcgcgtat ctgactatga cggttacaac cagtttgtcg ttcaccgttc accacagccg
     7081 ctgatgtccc cggcgtggtc accagacggt tctaaactgg cttatgtgac cttcgaaagc
     7141 ggtcgttccg cgctggttat acaaacgctg gcaaacggcg ctgtacgtca ggtggcttca
     7201 ttcccgcgtc acaacggtgc gcctgcattc tcgccagacg gcagcaaact ggcattcgcc
     7261 ttgtcgaaaa ccggtagcct gaacctgtac gtaatggatt tggcttctgg tcagatccgc
     7321 caggtgactg atggtcgcag taacaatacc gaaccgacct ggttcccgga cagccagaac
     7381 ctggcattta cttctgacca ggccggtcgt ccacaggttt ataaagtgaa tatcaacggc
     7441 ggtgcgccac aacgtattac ctgggaaggt tcgcagaacc aggatgcgga tgtcagcagc
     7501 gacggtaaat ttatggtaat ggtcagctcc aatggtgggc agcagcacat tgccaaacaa
     7561 gatctggcaa cgggaggcgt acaagttctg tcgtccacgt tcctggatga aacgccaagt
     7621 ctggcaccta acggcactat ggtaatctac agctcttctc aggggatggg atccgtgctg
     7681 aatttggttt ctacagatgg gcgtttcaaa gcgcgtcttc cggcaactga tggacaggtc
     7741 aaattccctg cctggtcgcc gtatctgtga taataattaa ttgaatagta aaggaatcat
     7801 tgaaatgcaa ctgaacaaag tgctgaaagg gctgatgatt gctctgcctg ttatggcaat
     7861 tgcggcatgt tcttccaaca agaacgccag caatgacggc agcgaaggca tgctgggtgc
     7921 cggcactggt atggatgcga acggcggcaa cggcaacatg tcttccgaag agcaggctcg
     7981 tctgcaaatg caacagctgc agcagaacaa catcgtttac ttcgatctgg acaagtacga
     8041 tatccgttct gacttcgctc aaatgctgga tgcacatgca aacttcctgc gtagcaaccc
     8101 gtcttacaaa gtcaccgtag aaggtcacgc ggacgaacgt ggtactccgg aatacaacat
     8161 ctccctgggt gaacgtcgtg cgaacgccgt taagatgtac ctgcagggta aaggcgtttc
     8221 tgcagaccag atctccatcg tttcttacgg taaagaaaaa cctgcagtac tgggtcatga
     8281 cgaagcggca tactccaaaa accgtcgtgc ggtactggtt tactaagaga attgcatgag
     8341 cagtaacttc agacatcaac tattgagtct gtcgttactg gttggtatag cggccccctg
     8401 ggccgctttt gctcaggcac caatcagtag tgtcggctca ggctcggtcg aagaccgcgt
     8461 cattcaactt gagcgtattt ctaatgctca cagccagctt ttaacccaac tccagcaaca
     8521 actctctgat aatcaatccg atattgattc cctgcgtggt cagattcagg aaaatcagta
     8581 tcaactgaat caggtcgtgg agcggcaaaa gcagatcctg ttgcagatcg acagcctcag
     8641 cagcggtggt gcagcggcgc aatcaaccag cggtgatcaa agcggtgcgg cggcatcaac
     8701 gacgccgaca gctgatgctg gtactgcgaa tgctggcgcg ccggtgaaaa gcggtgatgc
     8761 aaacacggat tacaatgcag ctattgcgct ggtgcaggat aaatcccgcc aggatgacgc
     8821 aatggtggca tttcagaatt tcatcaaaaa ttaccctgat tcaacttacc tgccaaacgc
     8881 caattattgg ctgggtcagt taaactacaa caagggtaaa aaagatgatg cggcgtacta
     8941 ttttgcttcg gtagtgaaaa actatccgaa gtcaccaaag gctgcagatg cgatgtttaa
     9001 agtcggcgtc atcatgcagg acaaaggtga caccgcaaaa gcgaaagccg tataccagca
     9061 ggttatcagt aaataccctg gtactgatgg cgctaaacag gcacaaaaac gtctgaacgc
     9121 gatgtaatgc ataacacacg accagaagtc gcattatttc tggtcgtgtc gtgcgaatca
     9181 taagcagttg agtgatctac atcgaaattt ttgttgcgct caagtctgaa atcagtaata
     9241 tatgccgccg ttgccacggg atatcaaaca aaccgaaagc aacgaaaaag tgggtcgtta
     9301 gctcagttgg tagagcagtt gacttttaat caattggtcg caggttcgaa tcctgcacga
     9361 cccaccacta acctagttag ttgtagtatc cagcgtaata tcgggtgatt agctcagctg
     9421 ggagagcacc tcccttacaa ggagggggtc ggcggttcga tcccgtcatc acccaccacc
     9481 gggtcgttag ctcagttggt agagcagttg acttttaatc aattggtcgc aggttcgaat
     9541 cctgcacgac ccaccactaa ttacggtggg ttcggtggaa gtagtttgta gtatccagcg
     9601 cagtatcggg tgattagctc agctgggaga gcacctccct tacaaggagg gggtcggcgg
     9661 ttcgatcccg tcatcaccca ccactcgggt cgttagctca gttggtagag cagttgactt
     9721 ttaatcaatt ggtcgcaggt tcgaatcctg cacgacccac caattttaac atcaaactca
     9781 gatgttaagc gtgaaggata acgttgcgtc agcaacggcc cgtagggcaa gcgaagcgag
     9841 tcatcctgca cgacccacca attttaaaga ttggttccga gtagaaatct ttcaggtaac
     9901 acccgtatgg gtcgttagct cagttggtag agcagttgac ttttaatcaa ttggtcgcag
     9961 gttcgaatcc tgcacgaccc accaatgtaa aaaagcgccc taaaggcgct tttttgctat
    10021 tcaggcatct ctcaatttca attcttgacc ctgatgacat actccgcgct tgctgcgtaa
    10081 acaactttac gtcggaggaa taagacgcaa agtgtggcat tcggcatctc atacgtaatt
    10141 cgagtgactt ttccccacca ttcgactatc ttgtttagca tataaaacaa attacaccga
    10201 taacagcgaa tatcacgcta atgtcggttt taacgttaag cctgtaaaac gagatggtaa
    10261 gatgagcgtg atgtttgatc cagacacggc gatttatcct ttccccccga agccgacgcc
    10321 gttaagcatt gatgaaaaag cgtattaccg cgagaagata aaacgtctgc taaaagaacg
    10381 taatgcggtg atggttgccc actactatac cgatcccgaa attcaacaac tggcagaaga
    10441 aaccggtggc tgtatttctg attctctgga aatggcgcgc ttcggtgcaa agcatcccgc
    10501 ttctacgttg ttagtcgctg gggtgagatt tatgggagaa accgccaaaa ttctcagtcc
    10561 ggaaaaaaca attctgatgc cgacacttca ggctgagtgt tcactggatc tcggctgccc
    10621 tgttgaagaa tttaacgcat tttgcgatgc ccatcccgat cgtactgtcg tcgtctacgc
    10681 caacacttct gctgcggtaa aagcgcgggc agattgggtg gtaacttcaa gcattgccgt
    10741 cgaacttatt gatcatctgg atagtttggg tgaaaaaatc atctgggcac ccgacaaaca
    10801 tctggggcgt tacgtgcaaa aaaagacggg cgcagacatt ttatgctggc agggtgcctg
    10861 cattgtgcat gatgagttta agactcaggc gttaacccgc ttgcaagaag aatacccgga
    10921 tgctgccata ctggtgcatc cagaatcacc acaagctatt gtcgatatgg cggatgcggt
    10981 cggttccacc agtcaactga tcgctgctgc gaaaacattg ccacatcaga ggcttattgt
    11041 ggcaaccgat cggggtattt tctacaaaat gcagcaggcg gtgccagata aagagttact
    11101 ggaagcacca accgcgggtg agggcgcaac ctgccgcagc tgcgcgcatt gtccgtggat
    11161 ggccatgaat ggcctccagg ccatcgctga ggcattagaa caggaaggaa gcaattacga
    11221 ggttcatgtt gatgaaaggc tgcgagagag ggcgctggtg ccgctcaatc gtatgctgga
    11281 ttttgcggct acactacgtg gataacgaat aataaggcgt aacgttacgc tttgggggaa
    11341 agatggattt ttttagtgtg cagaatatcc tggtacatat accaatagga gcaggcggtt
    11401 atgatctctc atggatcgaa gcggtaggca caatcgccgg gttgctgtgt attggccttg
    11461 ccagtctgga gaagatcagc aactacttct ttggcctgat caacgtcacc ttgtttggca
    11521 ttattttctt tcagattcag ctgtatgcca gcctgctatt acaggtgttt ttctttgccg
    11581 cgaatattta cggctggtat gcgtggtcgc gacaaaccag tcagaacgag gcggagttaa
    11641 aaattcgctg gttgccattg ccgaaggcac tcagctggtt ggcggtttgc gttgtttcga
    11701 ttggtctgat gacggtattt atcaatccgg tgtttgcatt tttgacccgc gtggcagtca
    11761 tcatcatgca agcattagga ttacaggttg cgatgcctga actacaaccg gacgctttcc
    11821 cgttctggga ttcttgcatg atggtgttat ctatcgtggc aatgattctg atgactcgta
    11881 agtatgtgga aaactggctg ttgtgggtga ttattaacgt gattagcgtc gttatttttg
    11941 cacttcaggg cgtttacgcc atgtctctgg agtacatcat cctgaccttt attgcgctca
    12001 acggcagccg gatgtggatc aatagcgcac gcgaaagagg ctcacgcgcg ctgtcccatt
    12061 aatggtgatg atgtgaatgg ccagacaccc cttcattcag gtggcagtct ggcccatgac
    12121 aaggttgata ttccatctga atggtggcgt gctcaatctg atagtgatcc atcaggtagt
    12181 gttggatctg atccaacaag gcatcgtgat cgtgtggcgg gatcacctgc acatgcaacg
    12241 tcatcaccgg cttctcgcct accatccata catgcacatg gtgcacattg cggacttccg
    12301 ggatttcccg gcacatacga cgcttcagtt cagcgatatc cagcgatacc ggtgcaccct
    12361 caagtaattc attcacgcta tctttcaaca gtcgccacgc gctacgcaga accagaagcg
    12421 acaccagtat cgagagaatg gggtcagcag gtgtccagcc ggtccagata ataatcaacg
    12481 cggcaataat agccccaacc gaacccagca ggtccccgag cacatgcagt gccgctgctc
    12541 gtacgttgag gtttttctct tcgctgccgt gatgaagtaa ccagaaagaa agtatatttg
    12601 ccagcaaccc agccacggca attgccatca tcatgccgcc ctcgaccgga cgcggcgtgc
    12661 ggaagcgttc tatcgcctcc cagacaatca aaatggtaat taccacgagg gcgatggcat
    12721 tcacaaacgc cgcgagcgtg gtcaatctta gccaaccgaa agtgtggcga atggtgggag
    12781 gacgacgtga aaattgcacg gcgagtaggg cgaaaagcag ggcggcagta tcggtcaaca
    12841 tatgacccgc atcggccagc aaagccagag aaccggaaag aaaaccacca atgacttcta
    12901 ccagcataaa cccggcagtc acgccgaaag catacaacaa gcgacgagca ttattatctt
    12961 ctggcaggtg tgaggacgtg tgtgagtgtg agtgcgccat gacgagtcat cccttaatta
    13021 ttctcactta actttatgac atcatactgc ttttagaagg gaaaaattaa aagggagaga
    13081 ctccgctctc ccattattgg ctattttgca gggttactgc gtggtaccgt cggttttggt
    13141 atcgacatca ttattgatgc catcaccggt ttgtaccttt ttattgatat ccgggcagcg
    13201 accatctttg cacatggtgt tcttgtgctc ttcatctttg gtcatgccgt cattgttcat
    13261 tgaagaacca tccggatgca gcatagtgcc gccagaaccg gtatttaccc cgttattgtc
    13321 gacgttattt ggcgcgacat tttcacgggc gtcaggggct acctggcccg catcagctgc
    13381 ggcgtttgcc tggccgttat tggtttgcgc tccgctatcg gcagccagtg cggcaccgct
    13441 ggcaaggctt agagtggcag tcagaaatag tgtggccagt tttgtcattt tcataggatg
    13501 ctcctgttat ggtcgttatg tcggataacc tcttccaaca gtgcatttgc aggtgaatat
    13561 aaggcattgg tttaagattt cagccaggca aagaaatcca gcagagaatc ttgaaataat
    13621 taacaaacaa aggagttaca gttagaaatt gtaggagaga tctcgttttt cgcgacaatc
    13681 tggcgttttt cttgctaatt ccaggattaa tccgttcata gtgtaaaacc ccgtttacac
    13741 attctgacgg aagatataga ttggaagtat tgcattcact aagataagta tggcaacact
    13801 ggaacagaca tgaattatca gaacgacgat ttacgcatca aagaaatcaa agagttactt
    13861 cctcctgtcg cattgctgga aaaattcccc gctactgaaa atgccgcgaa tacggttgcc
    13921 catgcccgaa aagcgatcca taagatcctg aaaggtaatg atgatcgcct gttggtggtg
    13981 attggcccgt gctcaattca tgatcctgtc gcggctaaag agtatgccac tcgcttgctg
    14041 gcgctgcgtg aagagctgaa agatgagctg gaaatcgtaa tgcgcgtcta ttttgaaaag
    14101 ccgcgtacta cggtgggctg gaaagggctg attaacgatc cgcatatgga taacagcttc
    14161 cagatcaacg acggtctgcg tatagcccgt aaattgctgc ttgatattaa cgacagcggt
    14221 ctgccagcgg cgggtgaatt cctcgatatg atcactcctc agtatctcgc tgacctgatg
    14281 agctggggcg caattggtgc acgtaccacg gaatcgcagg tgcaccgcga actggcgtct
    14341 ggtctttctt gtccggtagg ttttaaaaat ggcacagacg gtacgattaa agtggctatc
    14401 gatgccatta atgccgccgg tgcgccgcac tgcttcctgt ccgtaactaa atgggggcat
    14461 tcggcgattg tgaataccag cggtaacggc gattgccata tcattctgcg cggcggtaaa
    14521 gagcctaact acagcgcgaa gcacgttgct gaagtgaaag aagggctgaa caaagcaggt
    14581 ctgccagctc aggtgatgat cgatttcagc catgctaatt cgtccaaaca attcaaaaag
    14641 cagatggatg tttgtgctga cgtttgccag cagattgccg gtggcgaaaa ggccattatc
    14701 ggtgtgatgg tggaaagtca tctggtggaa ggcaatcaga gcctggagag cggggagccg
    14761 ctggcctatg gtaagagcat caccgatgcc tgcattggct gggaagatac cgatgctctg
    14821 ttacgtcaac tggcgaacgc agtgaaagcg cgtcgcgggt aaagtttaat tgtcggatgc
    14881 gccgtcagag tggcgtatcc gatgaatcac cgtagccctg acaagacgcg cagcgtcgca
    14941 tcaggcaatg tgctccattg ttagcgacga caaacaaaaa agccgactca cttgcagtcg
    15001 gctttctcat ttttaaacga atgacgttta cttcgcttta ccctggttcg caacggctgc
    15061 tgctttcgcc gcgatctcgt cagcattacc cagataatag cgtttcagcg gtttgaagtt
    15121 ctcgtcaaac tcataaacca gcggcacgcc ggtcgggata ttcagctcaa gaatctcttc
    15181 ttcgctcatg ttatcgagat atttcaccag cgcacgtaac gagttaccgt gtgcagcgat
    15241 gattacgcgc tcaccgctct tcatacgcgg cagaatggtt tcattccagt aagggatcac
    15301 gcggtcaatg gtcagcgcca ggctttccgt cagcggcagt tctttctcgc tcagtttcgc
    15361 gtaacgcgga tcgtgacccg gataacgctc atcatcttta gtcagttccg gcggagtcac
    15421 tgcaaaacca cgacgccact gtttcacctg ctcgtcgcca tatttttcag cagtttccgc
    15481 tttgttcaga ccctgcaacg caccgtagtg acgttcgttc agtttccagg atttctcaac
    15541 gggcagccat gcctgatcca gttcgtccag cacattccac agggtatgga tagcgcgttt
    15601 cagcacagag gtgtaagcaa agtcaaagct gtaaccttcc tctttcagca gcttacctgc
    15661 tgcttttgct tcgcttacgc ctttctcaga cagatccacg tcgtaccaac cggtgaaacg
    15721 gttttctttg ttccactgac tttcgccatg acgaaccaga accagcttag ttacagccat
    15781 atacttactc ctcaaatcat cttttaatga taataattct cattatattg ccgtgacgaa
    15841 gcaacagcaa tgcttacgca taaccatagc gaaaatagtg gcgcagtgta aggttgttgt
    15901 gaatattgag ttgcaaatat gtcggtgttt gctggtgatt tgaacaatat gagataaagc
    15961 cctcatgacg agggcttaac attactcagc aataaactga tattccgtca ggctggaata
    16021 ctcttcacca ggatgcagga aacagtccgg ttgcggccat tcagggtggt tcgggctgtc
    16081 cggcagaaac tcgctttcca acgccagccc ttgccagtcg gcgtaaggtt cggttccccg
    16141 cgacggtgtg ccgccgaggt agttgccgga gtagaattgc agagccggag cggtggtgta
    16201 gaccttcagc tgcaattttt catctgctga ccagacatgc gccgccactt tcttgccatc
    16261 gcctttggcc tgtaacaaga atgcgtgatc gtaacctttc actttgcgct gatcgtcgtc
    16321 ggcaagaaac tcactggcga tgattttggc gctgcggaaa tcaaaagacg ttccggcgac
    16381 agatttcagg ccgtcgtgcg gaataccgcc ttcatcaacc ggcagatatt cgtccgccag
    16441 aatctgcaac ttgtgattgc gcacgtcaga ctgcttgccg tcaagattga aatagacgtg
    16501 attagtcata ttcaccgggc aaggtttatc aactgtggcg cgataagtaa tggagatacg
    16561 gttatcgtcg gtcagacgat attgcaccgt cgcgccgaga ttacccggga agccctgatc
    16621 gccatcatct gaactcaggg caaacagcac ctgacgatcg ttctggttca caatctgcca
    16681 gcgacgtttg tcgaaccctt ccgggccgcc gtgcagctgg ttaacgccct gacttggcga
    16741 aagcgtcacg gtttcaccgt caaaggtata acgactattg gcgatgcggt tggcataacg
    16801 accaatagag gcacccagaa acgcggcctg atcctgatag cattccgggc tggcacagcc
    16861 gagcagcgcc tcgcggacgc tgccatcgga aagcggaata cgggctgaaa gtaaagtcgc
    16921 accccagtcc atcagcgtga ctaccatccc tgcgttgtta cgcaaagtta acagtcggta
    16981 cggctgacca tcgggtgcca gtgcgggagt ttcgttcagc actgtcctgc tccttgtgat
    17041 ggtttacaaa cgtaaaaagt ctctttaata cctgtttttg cttcatattg ttctgcgaca
    17101 gcttgctgta cggcaggcac cagctcttcc gggatcagcg cgacgataca gccgccaaat
    17161 ccgccgccgg tcatgcgtac gccacctttg tcgccaatca cagctttgac gatttctacc
    17221 agagtgtcaa tttgcggcac ggtgatttcg aaatcatcgc gcatagaggc atgagactcc
    17281 gccatcaact cgcccatacg tttcaggtcg ccttgctcca gcgcgctggc agcttcaacg
    17341 gtgcgggcgt tttcagtcag gatatgacgc acgcgtttcg ccacgattgg gtccagctca
    17401 tgtgcaacag cgttgaactc ttcaatggtg acatcgcgca gagctggctg ctggaagaaa
    17461 cgcgcaccgg tttcgcactg ttcacgacgg gtgttgtatt cgctgccaac cagggtacgt
    17521 ttgaagttac tgttgatgat gacgacagcc acacctttcg gcatggaaac tgctttggtc
    17581 cccagtgagc gacagtcaat cagcaaggca tgatctttct tgccgagtgc ggaaattagc
    17641 tgatccatga tcccgcagtt acagccaaca aactggtttt ctgcttcctg accgttaagc
    17701 gcgatttgtg cgccgtccag cggcagatga taaagctgct gcaatacggt tccgaccgcg
    17761 acttccagtg aagcggaaga acttaacccg gcaccctgcg gcacattgcc gctgatcacc
    17821 atgtccacac cgccgaagct gttgttacgc agttgcagat gtttcaccac gccacgaacg
    17881 tagttcgccc attgatagtt ttcatgcgcg acaatgggcg catcaaggga aaactcatca
    17941 agctgatttt cataatcggc tgccattacg cgaactttac ggtcatcgcg tggcgcacag
    18001 ctgatcacgg tttgataatc aatcgcgcag ggcagaacga aaccgtcgtt gtagtcggtg
    18061 tgttcaccaa tcaaattcac gcggccaggc gcctgaatgg tatgagtggc agggtagcca
    18121 aatgcgttgg caaacagaga ttgtgttttt tctttcagac tcatttctta cactccggat
    18181 tcgcgaaaat ggatatcgct gactgcgcgc aaacgctctg ctgcctgttc tgcggtcagg
    18241 tctcgctggg tttctgccag catttcataa ccaaccataa atttacgtac ggtggcggag
    18301 cgcaacagag gcggataaaa gtgcgcgtgc agctgccagt gttgattctc ttcgccatta
    18361 aacggtgcgc cgtgccagcc catagagtag gggaaggagc actggaagag gttgtcataa
    18421 cgactggtca gctttttcaa cgccagtgcc aaatcgctgc gctgggcgtc ggtcaaatcg
    18481 gtgatccgca aaacgtgggc tttgggcaga agtagcgttt cgaacggcca ggcagcccag
    18541 taaggcacaa ctgctaacca gtgttcggtt tcgacaacgg tacggctacc gtctgccagc
    18601 tcgcgctgaa cataatccac cagcattggc gatttctgct cggcaaaata ttctttttgc
    18661 aggcggtctt cgcgctcagc ttcgttaggc aggaagctat ttgcccaaat ctgtccgtgc
    18721 ggatgcgggt tagagcagcc catcgccgca cctttgtttt caaagacctg cacccacggg
    18781 tatgttttcc ccagttctgc ggtttgctcc tgccaggttt tgacgatttc cgtcaatgcc
    18841 gcaacgctca gttctggcag cgttttactg tgatccggtg aaaagcagat cacccggctg
    18901 gtaccgcgcg cgctctggca acgcatcaaa ggatcgttgc tttctggcgc atctggcgtg
    18961 tcagacatca aggccgcaaa gtcattagtg aaaacgtaag tcccggtgta atcggggttt
    19021 ttatcgcctg tcacccgcac attacctgcg cagaggaagc aatctggatc gtgcgcaggt
    19081 aacacctgtt tggctggcgt ttcctgcgcc ccctgccagg ggcgcttagc gcggtgcggt
    19141 gaaaccagaa tccattgccc ggtgagctgg ttgtagcggc gatgtggatg atcaacggga
    19201 ttaaattgcg tcatggtcgt tccttaatcg ggatatccct gtggatggcg tgactgccag
    19261 tgccaggtgt cctgcgccat ttcatcgagt gtgcgcgtta cgcgccagtt cagttcacgg
    19321 tcggctttgc tggcgtccgc ccagtaggcc ggaaggtcgc cctcgcgacg cggtgcaaaa
    19381 tgatagttaa ccggtttgcc gcaggctttg ctgaaggcat taaccacgtc cagcacgctg
    19441 ctgcctacgc cagcaccgag gttgtagatg tgtacgcctg gcttgttcgc cagtttttcc
    19501 atcgccacga cgtgaccgtc cgccagatcc attacgtgga tgtaatcgcg tacgccagta
    19561 ccgtcttcgg tcggataatc gttaccaaaa atcgccagcg agtcgcgacg gcctacagca
    19621 acctgggcga tgtatggcat aaggttattc ggaatgcctt gcggatcttc gcccatatcg
    19681 cccgacggat gtgcgccaac cgggttgaag tagcgcagca gggcaatgct ccagtccggc
    19741 tgggcttttt gcagatcggt gaggatctgt tccaccatca gcttgctttt gccgtaaggg
    19801 ctttgcggtg tgccggtcgg gaagctttca acgtatggaa ttttgggctg atcgccataa
    19861 acggtggcgg aggagctaaa aataaagttt ttgacgttag cggcgcgcat ggcgctaatc
    19921 aggcgcagag taccgttgac attgttgtcg taatattcca gcggtttttg taccgattcg
    19981 ccaacggctt tcagcccggc gaagtggatc acggtgtcga tagcgtgatc gtgcaggatc
    20041 tcggtcatca acgcttcgtt acggatatcg ccttcaacaa acgtcggatg tttgccgcct
    20101 aaacgctcga taacaggcag tacgctgcgc ttactgttac agaggttatc aagaatgatg
    20161 acatcatgac cgttttgcag taattgcaca caggtatgac ttccaatgta accgctacca
    20221 ccagtgacca gaactctcat aattcgctcc attaggctta tggtatgaaa taaccatagc
    20281 ataacaaaga tgcgaaaagt gtgacatgga ataaattagt ggaatcgttt acacaagaat
    20341 ttagccgttt tttatgcgcg attaagtgat tataaaacag agggattgtg aatgattgcg
    20401 ctttttatct gaaaaaaaga cgcagtttca tgcctggatg cgtctgaccg tgtgccggat
    20461 aaagcgttta cgccgcatcc ggcacagtca gcattaccga cttagtttat ttttgtcagc
    20521 gcataatgat agaggccacc gtccggcacg aactccagac gatgggtaat acaagccggc
    20581 gcatcttcag catggtgcga aacaaacaac aattgcgttt caccttcgct aatcagcaca
    20641 tcaacaaaac ggcgaataag ctggcgattg agcggatcaa gcccctgcaa tggttcgtcg
    20701 agaataagca acgtcggatg tttcaccagt gcgcggacga tcagcgccag acgctgctgt
    20761 ccccaggaaa gactatggaa cggagcgtca gccgtgcgtt tatcaatgcc gagaatatcc
    20821 agccactgct gcaccagttt ttgctggcga tccgaaacgg cctgataaat gccaatcgaa
    20881 tcaaaatagc cagaaagaat cacattacgc acagtagtac tgacccggta atccagatgc
    20941 aaactactgc tgacgtaacc gatatgcttt ttgatatccc agatggtttc gccgctgcca
    21001 cgacgtcgtc cgaaaagcgt caaatcgttg ctgtaacctt gcggatgatc gccagtaatc
    21061 aggcttaata acgtcgattt tcccgcgcca tttggcccga caatttgcca gtgttcacct
    21121 ggattcacct gccagctaag gttattaaga atggggcgat cgttataaga aaccacgcca
    21181 ttattcagca caatgcgcgg ttcgttggcg ggtaaggcgt gacgtgctga aggttcatcc
    21241 ggctccggca gttgcacacc ttcaagctgt tcactatgcg ccagttgcgc gacgagtgct
    21301 tgttggagca gttcctcttt agcgccagtt tccgctaacg tgcaatccgc cagcacgcca
    21361 gcaaactgga caaactccgg gatctcatcg aagcgattga gcaccagtac cagagtaata
    21421 ccggactgat gtaacgaggc gagtcgctca gccagctgct gacgtgaggc aacatccagg
    21481 ccatcgaacg gctcatcaag aatcaacaag tcaggctccg acatcagcgc ctgacacagc
    21541 agggtttttc gcgtctcgcc agtggaaagg tatttaaagc gtcggtcgag gaggggggta
    21601 ataccgaact gctgtgccag ttgcatgcag cgcggtgcat cctttacttc atcctgaata
    21661 atctcagccg tagtgcgtcc ggtgtcatct tcgccagggc cgagcatatc ggtgttattc
    21721 cgctgccatt cgtcgctgac gagcttttgc aattgctcga aggagagacg agtgatgtgg
    21781 gaaaactggc tttgccgttc acctttcaaa agcggaagtt cccccgccag cgcgcgggcc
    21841 agggccgatt tcccgcttcc attcgaaccg acaaacgccc aactatcacc cgcgtttaac
    21901 gttagctgag gcaattgcag cgtttttgtg tcgctaagac gaaacgtgcc ttgcaaaatt
    21961 tgcaacgatg acattttata tcccattttg tgcagcgatt actgacaggg atacgtgttt
    22021 cataacaaat tgtcaacacg cttagcacag tgtggcgata atcacgctgt cggcattaaa
    22081 gtaggccgtg acattctgtc cttgctgaag agaagtcgct tcatttaccg gcactgtggc
    22141 gcacagtgtt tgcccgtccg gtagcgccat taatacttcg cactgctctg cgccgcgctc
    22201 aatatgacta ataatacccg gtaattggtt gtcagcgttt tgcgcgaccg cttcgtcctg
    22261 agtaatgcct acccacggcg cttttagcaa tatcaacact tctttgcctt catccagccc
    22321 cagacgcgcg ccgctttgtg cggtaattgc gactttcagg cgtgtttttc catcagccag
    22381 taagacatca acatgctgtt gaacgtcatc atgatcgcgg gcggtgatgg taccgaacca
    22441 ctggttacgg gcgctggttt ggagtgaaaa acgtgagatc gcggccagca ggctgttcag
    22501 cggcagggcg tcatcgtcac ttaacacatc aaaggctttt tgctggattt gcgccagtaa
    22561 gtcatagagc tgaatcagtc gctgaccata gcgggtcagt actgcgccgc cgccaccttt
    22621 accgcctgtt gcgcgctcga ccagaatatg ctcacttaac tgattcatct cgttaatggc
    22681 atcccaggcg cttttatagc tgataccggc atctttcgct ccctggctaa tggaaccgga
    22741 aagcgcaatg tgttttagta gcgaaatgcg gcgcgggtcg gcgaataatt tttgttggag
    22801 cttaagggtg agaaggattt cggcctgcat aacaatgtcc tggcaaaagt cttattgtga
    22861 cggaaaacga acgccacgca aagctgaccg cacaaaaggg gagtgctttt ctgtgcttag
    22921 cggttagaat agtctcatga ctatatctgg agttgaccat gttagagtta ttaaaaagtc
    22981 tggtattcgc cgtaatcatg gtacctgtcg tgatggccat catcctgggt ctgatttacg
    23041 gtcttggtga agtattcaac atcttttctg gtgttggtaa aaaagaccag cccggacaaa
    23101 atcattgatt ccctgaatgc ccgcttagtc gggcattttc tttttctcaa cttcctgctt
    23161 ttcctgccga tattttttct tatctacctc acaaaggtta gcaataactg ctgggaaaat
    23221 tccgagttag tcgttatatt gtcgcctaca taacgttaca ttaaggggtt accaatggct
    23281 cgtaaatggt tgaacttgtt tgctggggcg gcactctctt tcgctgttgc tggcaatgca
    23341 ctggcagatg aagggaaaat cacggtgttc gccgccgcat cactgactaa cgcaatgcag
    23401 gacattgcta cgcagtataa aaaagagaaa ggcgtggatg tggtttcttc tttcgcttcg
    23461 tcatctactc tcgcccgtca gattgaagcg ggtgcccctg cggatctgtt tatttctgcc
    23521 gatcagaaat ggatggatta tgcggttgat aaaaaagcga tcgatacagc tacgcgtcag
    23581 acactgctcg gcaatagcct ggtcgttgta gcaccgaaag ccagcgagca gaaagatttt
    23641 accatcgaca gcaaaaccaa ctggacttca ctgctgaatg gcggtcgcct ggcggttggc
    23701 gatccggaac atgttcccgc tggcatttat gcaaaagaag cactgcaaaa actgggcgca
    23761 tgggatacgc tctccccgaa actggctccg gcggaagatg ttcgtggcgc gctggcgttg
    23821 gtcgaacgca atgaagcgcc gttgggcatt gtttacggtt ctgacgcagt tgctagcaaa
    23881 ggggtaaaag tggttgccac cttcccggaa gattcacata aaaaagtgga atatccggtt
    23941 gctgttgtgg aagggcataa caatgcgaca gtgaaagcat tttatgatta tctgaaggga
    24001 ccgcaggcag cggaaatctt taaacgttac ggatttacaa ccaagtaatg atactgaccg
    24061 atccagaatg gcaggcagtt ttattaagcc tgaaagtttc ttccctggct gtgctgttta
    24121 gcctgccgtt tgggatcttt tttgcctggt tactggtgcg ttgcacgttt ccgggcaaag
    24181 ctctgctcga cagcgtactg catctaccgc tggtgttacc gcccgtggtc gtcggttact
    24241 tattattagt ttcgatgggg cggcgcggat ttatcggtga acgtctgtat gactggtttg
    24301 gtattacctt cgcctttagc tggcgcggcg cggttctcgc tgccgccgtc atgtcgtttc
    24361 cgctgatggt gcgggcaatt cgtctggcgc tggaaggggt tgatgtcaaa ctggaacagg
    24421 ccgcaagaac actgggggcc gggcgctggc gcgttttctt tactatcacg ttaccgctga
    24481 ccttaccggg aattattgtt ggtacggtac tggcttttgc tcgttctctc ggtgaatttg
    24541 gcgcaaccat cacctttgtg tcgaacattc ctggtgaaac gcgaaccatt ccttctgcca
    24601 tgtataccct gattcagacc cccggcgggg aaagtggcgc agcgagactg tgcattattt
    24661 ctattgcgct ggcgatgatc tccctgttga tttcagaatg gctggccaga atcagccgtg
    24721 aacgggcggg gcgctaatca tgctggaact gaatttttcc cagacgttgg gcaaccattg
    24781 cctgactatt aatgaaacgc tgcccgccaa tggcatcact gctatctttg gcgtctccgg
    24841 tgccggaaaa acttcgctga ttaacgccat cagtggactg acgcgcccgc aaaaagggag
    24901 gattgtcctc aatggtcggg tactaaatga tgccgaaaaa ggtatctgcc tgacgccgga
    24961 aaagcgtcgc gttggctatg tttttcagga tgcgcggctg ttcccgcatt acaaagtgcg
    25021 tggcaatctg cgctacggca tggcgaaaag catggtcgat cagttcgata agctggtggc
    25081 gcttttaggc attgaaccgt tgcttgaccg tttaccaggc agcctgtccg gaggcgaaaa
    25141 acagcgcgta gcgattgggc gggctttgct gacggcacca gaattgctgt tgctggatga
    25201 accgctggcg tcactggata ttccgcgtaa acgcgaactg ttgccttatc tgcaacgtct
    25261 gacgcgggaa atcaacatcc cgatgttgta tgtcagccat tcgctggatg agatcctcca
    25321 tctggcagac agagtgatgg tactggaaaa cggtcaggtg aaagcctttg gcgcgctgga
    25381 agaagtctgg ggtagtagcg tgatgaatcc gtggctgccg aaagagcaac aaagtagcat
    25441 tctgaaagtg acggtacttg agcatcatcc gcattacgcg atgaccgcgc tggcgctggg
    25501 cgatcagcat ttgtgggtca ataagctgga cgaaccgctg caagctgcgc tacgtatccg
    25561 cattcaggct tccgatgttt ctctggtttt acaaccgccg cagcaaacca gcattcgtaa
    25621 cgtattgcgg gcaaaagttg ttaatagtta tgacgacaac ggccaggtgg aagtggaact
    25681 ggaagtcggc ggtaaaacgc tgtgggcgcg tatcagcccg tgggccaggg atgaactggc
    25741 gatcaaacct ggcctgtggc tgtacgcgca aattaaaagt gtgtcgataa ccgcctgatt
    25801 aaatcaggtg gctataaatg aactgggcaa tgctgtcggt ggtgttatca ccaatcacaa
    25861 tgttggcgcg cgcttttacc gcgtcatcgg cgttgcccat cgccacgcct gtaccagcgg
    25921 cctccagcat actgatatca ttaaagttat cgccgaacgc cacgacgttt tccatcgacc
    25981 agccttgcgc ctcaacccat ttcgtcaaac gtttaccttt gctgttgccg ccgcgtgcaa
    26041 tatcaacctg atcgtgccag gaccattcac actccagtcc cagttcatgt tcgacatgct
    26101 taccaaaatg ctgcaattgc ggcaggtcat cgtgcgtgag ggcgaacttc catacggcgt
    26161 taacttgttg cgccgtttca gccagagaag cgacttgtgt gaaagtcgga cgctgttccg
    26221 gcggcagggt ttgcgcccag ttagacgtgc gaatgacatg cccggtcggg tgctcataaa
    26281 ccatctcgtc atcgacatac atcagaccgt gaatgtggtg ttcattcagc atctcaatga
    26341 gttgtagggc tttattaacg ggcattgggt ccgcttccag cacggttttt gcatgataat
    26401 catacaaata ggtgccatta cagcaaatag caggtgtatc cagcgccagc gcctgataaa
    26461 aaggatgaat agcgacgtga tggcgacctg tgacgatgat taattgataa cctgcttcgc
    26521 gagcgcgggc cagggcttct atcgatgaag gtagcagggt ctttttcggg gtcaataagg
    26581 tgccgtctaa gtcgagagca atcacgcgtg tggtcatggc gtattccaga ttaaggttaa
    26641 gaattttctt ctgcgcgaat ggtacaccga taccactatc ggacaaaatt ctgcgtttta
    26701 attcagcatt caccgccaaa agcgactaat tttagctgtt acagtcagtt gctaaatgca
    26761 aaggagcatt catgaagcaa acagtttata tcgccagccc tgagagccag caaattcacg
    26821 tctggaacct gaatcatgaa ggcgcactga cgctgacaca ggttgtcgat gtgccggggc
    26881 aggtgcagcc gatggtggtc agcccggaca aacgttatct ctatgttggt gttcgccctg
    26941 agtttcgcgt cctggcgtat cgtatcgccc cggacgatgg cgcactgacc tttgccgcag
    27001 agtctgcgct gccgggtagt ccgacgcata tttccaccga tcaccagggg cagtttgtct
    27061 ttgtaggttc ttacaatgcg ggtaacgtga gcgtaacgcg tctggaagat ggcctgccag
    27121 ttggcgtcgt cgatgtggtc gaggggctgg acggttgcca ttccgccaat atctcaccgg
    27181 acaaccgtac gctgtgggtt ccggcattaa agcaggatcg catttgcctg tttacggtca
    27241 gcgatgatgg tcatctggtg gcgcaggacc ctgcggaagt gactaccgtt gaaggggccg
    27301 gcccgcgtca tatggtattc catccaaacg aacaatatgc atattgcgtc aatgagttaa
    27361 acagctcagt ggatgtctgg gaactgaaag atccgcacgg caatatcgaa tgtgtccaga
    27421 cgctggatat gatgccggaa aacttctccg acacccgttg ggcggctgat attcatatta
    27481 ccccggatgg tcgtcactta tatgcctgtg accgtaccgc cagcctgatt actgtcttca
    27541 gcgtttcgga agatggcagc gtgttgagta aagagggctt ccagccaact gaaacccagc
    27601 cgcgcggctt taatatcgat caccgtggta agtatctgat tgccgccggg caaaaatcgc
    27661 accacatctc ggtatacgaa attgttggtg agcaggggct actgcatgaa aaaggccgct
    27721 acgcggtcgg gcagggacca atgtgggtgg tggttaacgc acactaaccg ctgatttacc
    27781 cggcgcagtc tctcctgcgc cggtgtatta acctatctcc tgtaacgcat gtctctggcg
    27841 ttcgacgata ttggtccaca aattgtcttt atcgtcagtc cacaaattaa tcagcaaggc
    27901 aaaaaagcgt tctgctgccg gagaaagcac ggcatctttg cggcgaataa tccccaatgt
    27961 ccgacgtata acgggttcca ccagtgggat accaataata gaagaatagg gcgcatgggg
    28021 cattgccaga ccaggaagcg ccgaaattcc cagtcccgcc tctaccagtc ctaatgacgt
    28081 cgaaagatga cgcacttcgt aaaaccagtc cagcttccag ggcttgtcgg ctagctgttg
    28141 ttctatcaac aatcggttgc cactggagga acgtacgcca atcattttgt aacccactaa
    28201 ctcctgccat tctacaagct gctttttagc caatggatgg tcacgccgac aggccagcac
    28261 gaacggctcg ttgaccagtg gggtaaaatc aatggatgaa tttgtgacgt tgttcatgtt
    28321 tatgccaaag tctgattcgt tacacagcac cgattccatg caattattcg tgccttgttc
    28381 cagaatccgc actttaatat tgggatacag ctcattaaat ttaccgattg ccagcggtaa
    28441 aaaataaaat actgcggtcg gaatacacgc taacgtcacc ataccacgat gataagcatt
    28501 catatcacga atattaaaaa gcgtttcatc gaatttcttt attaattccc gcgcctctgg
    28561 gagcaacctt tttcctgctt ttgttaacgt aaccttgcga gttgtacgct caaatagttg
    28621 aatatgtaaa tcttcctcca tcttttttat tcttcgtgtt aatgcaggtt gcgtaatatt
    28681 gagtaattta gcggcattgt taaatgaact ggactctgcc agtatgacaa atgccttcat
    28741 acttgataat tcatgcttca ttattactcc gggaaatgga agcgacgatt ttgggtggct
    28801 ggccgttaaa aattttaact gcatttagcc aacttaaatt aatgaaaaaa tgttattaat
    28861 cgttgagcta aagtcattag agatgctttg cccttaatgt aaccatatcg caataagtta
    28921 tgtttttaaa ttgagggcat tattatgaaa aaaataccct gcgtgatgat gcgaggtgga
    28981 acctcgaggg gcgcgttcct gttagcggaa catttacccg aagatcaaac gcagcgcgat
    29041 aaaatattga tggcaattat gggttccggt aacgatctgg aaattgacgg tattggcggc
    29101 ggtaatccac tgaccagtaa agtcgccatt attagccgtt ccagcgatcc gcgtgctgat
    29161 gttgattatc tgtttgctca agttatcgtc catgagaaac gtgtcgatac cacgcctaac
    29221 tgcggcaata tgctgtctgg cgttggggca ttcgccattg aaaatggttt gattgcagcg
    29281 acttcgccag ttacccgcgt acgtatccgc aacgtcaata cgggtacgtt catcgaagct
    29341 gatgtgcaaa cgccaaatgg tgttgtcgag tacgagggta gcgccagaat tgacggcgta
    29401 ccgggtactg ccgcaccggt tgcgctcact ttcctgaatg ccgctggaac gaaaaccgga
    29461 aaagttttcc cgactgataa tcagattgat tattttgacg atgtcccggt gacctgtatc
    29521 gatatggcga tgccagtcgt cattattccg gctgaatatc tgggaaaaac aggctatgaa
    29581 ttaccggcgg aactggatgc cgacaaagca ttattagccc gcattgaatc tatccgtcta
    29641 caagcgggta aagcaatggg cttaggcgat gtcagtaata tggttatccc taaacctgtg
    29701 cttatttctc cagcgcagaa aggcggggca attaatgtgc gttattttat gccgcattct
    29761 tgccatcgcg cgctggcgat aaccggtgct attgctattt ccagtagttg tgcattggaa
    29821 ggtaccgtca cccgacaaat cgtcccttct gtaggatacg gcaatatcaa tattgaacac
    29881 cccagtggtg ggctcgacgt tcatttaagt aatgaaggtc aggatgccac gacgttacgc
    29941 gcatctgtta ttcggacaac cagaaaaata ttttccggtg aagtttatct tccctgaaaa
    30001 gattcgttgt caggataagg acaatcaata aaggacttct gtatgagtca tacagaaaga
    30061 acaggatttt aaatgaataa gaaatcgtta tggaagctaa ttctgatatt agcgatccca
    30121 tgtattattg gctttatgcc agctccggca ggattaagcg aactggcgtg ggtgcttttt
    30181 ggtatttacc tggcggccat tgtggggctg gttatcaagc ctttcccgga acctgtcgta
    30241 ctgttaattg ccgttgctgc ctccatggtg gtggtcggta acttatccga cggtgcgttt
    30301 aagaccaccg ccgtattaag cggttactct tcaggtacta cctggctggt gttctcggcg
    30361 tttaccttaa gcgccgcatt tgtgaccacc ggtttaggta aacgtattgc ctatctgctg
    30421 attggtaaaa tcggtaacac cacgctgggt ctgggttacg ttacggtatt cctcgatctg
    30481 gtattggctc cggcaacacc gtctaacacc gcgcgtgcgg gcggcattgt gttaccgatc
    30541 atcaacagcg tggcggtggc tttggggtcc gaaccggaaa aaagtccgcg tcgtgtcgga
    30601 cattacctga tgatgtccat ttacatggtc accaaaacca ccagctatat gttctttacc
    30661 gcaatggcgg ggaacattct ggcgctgaaa atgatcaacg acattctgca cctgcaaatt
    30721 agctggggtg gatgggcgct ggccgccgga ttgccgggca tcattatgct gctggtcacc
    30781 ccgctggtga tttacaccat gtatccacca gaaattaaga aggtggataa caaaaccatc
    30841 gctaaagcgg gccttgccga actgggaccg atgaaaatcc gcgaaaaaat gctgctcggt
    30901 gtctttgtgc tggcgctgct gggctggatt ttcagtaagt ctctgggggt tgatgaatct
    30961 tcagtggcta ttgttgttat ggcgaccatg ctgctgctgg gtatcgttac ctgggaagac
    31021 gtggttaaaa ataaaggcgg ctggaatacc ttaatctggt acggcggtat tatcggctta
    31081 agctccttat tatcgaaagt taaatttttc gaatggttag ctgaagtctt taaaaataac
    31141 ctggcatttg atggtcacgg taacgttgct ttcttcgtta ttattttcct cagcatcatc
    31201 gtgcgttatt tcttcgcttc cggtagtgcc tatatcgttg ctatgttacc ggtatttgcc
    31261 atgctggcga acgtctccgg cgcaccgtta atgttaaccg cgctggcact gttgttctcc
    31321 aactcctatg gcggcatggt tactcactat ggcggcgcgg caggtccggt catctttggc
    31381 gtgggttata acgatattaa atcctggtgg ttggtcggtg cggtactgac gatattaacc
    31441 ttcctggtgc atatcaccct cggcgtgtgg tggtggaata tgctgatcgg ctggaacatg
    31501 ctgtaaatat actcgtcata cttcaagttg catgtgctgc gtctgcgttc gctcacccca
    31561 gtcacttact tatgtaagct cctggggatt
//
LOCUS       DAZKIP010000047        31184 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZKIP010000047 DAZKIP010000000
VERSION     DAZKIP010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.047"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..599
                     /locus_tag="ACHOUK_002367"
     CDS             <1..599
                     /locus_tag="ACHOUK_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFP9897809.1"
                     /translation="DSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVG
                     EEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGT
                     IKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDN
                     IKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            829..1212
                     /gene="secE"
                     /locus_tag="ACHOUK_002368"
     CDS             829..1212
                     /gene="secE"
                     /locus_tag="ACHOUK_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFP9897810.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1214..1759
                     /gene="nusG"
                     /locus_tag="ACHOUK_002369"
     CDS             1214..1759
                     /gene="nusG"
                     /locus_tag="ACHOUK_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFP9897811.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1918..2346
                     /gene="rplK"
                     /locus_tag="ACHOUK_002370"
     CDS             1918..2346
                     /gene="rplK"
                     /locus_tag="ACHOUK_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFP9897812.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2350..3054
                     /gene="rplA"
                     /locus_tag="ACHOUK_002371"
     CDS             2350..3054
                     /gene="rplA"
                     /locus_tag="ACHOUK_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFP9897813.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3346..3843
                     /gene="rplJ"
                     /locus_tag="ACHOUK_002372"
     CDS             3346..3843
                     /gene="rplJ"
                     /locus_tag="ACHOUK_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFP9897814.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3910..4275
                     /gene="rplL"
                     /locus_tag="ACHOUK_002373"
     CDS             3910..4275
                     /gene="rplL"
                     /locus_tag="ACHOUK_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFP9897815.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4595..8623
                     /gene="rpoB"
                     /locus_tag="ACHOUK_002374"
     CDS             4595..8623
                     /gene="rpoB"
                     /locus_tag="ACHOUK_002374"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFP9897816.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8700..12923
                     /gene="rpoC"
                     /locus_tag="ACHOUK_002375"
     CDS             8700..12923
                     /gene="rpoC"
                     /locus_tag="ACHOUK_002375"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFP9897817.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            13136..13579
                     /locus_tag="ACHOUK_002376"
     CDS             13136..13579
                     /locus_tag="ACHOUK_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase growth adaptation protein"
                     /protein_id="HFP9897818.1"
                     /translation="MKQEVEKWRPFKHPDGDVRDLSFLDAHQVIYVQHHEDKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKLSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVVFRAFSEIFHHCLKLIEK"
     gene            complement(14014..15147)
                     /gene="thiH"
                     /locus_tag="ACHOUK_002377"
     CDS             complement(14014..15147)
                     /gene="thiH"
                     /locus_tag="ACHOUK_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFP9897819.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDSWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDERRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(15144..15914)
                     /gene="thiG"
                     /locus_tag="ACHOUK_002378"
     CDS             complement(15144..15914)
                     /gene="thiG"
                     /locus_tag="ACHOUK_002378"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFP9897820.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLAPQSGPGSRSYFAHATSPLTGFLEASV"
     gene            complement(15916..16116)
                     /gene="thiS"
                     /locus_tag="ACHOUK_002379"
     CDS             complement(15916..16116)
                     /gene="thiS"
                     /locus_tag="ACHOUK_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFP9897821.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLVQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(16100..16855)
                     /gene="thiF"
                     /locus_tag="ACHOUK_002380"
     CDS             complement(16100..16855)
                     /gene="thiF"
                     /locus_tag="ACHOUK_002380"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFP9897822.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWGQGCYRCLWPENQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16848..17483)
                     /gene="thiE"
                     /locus_tag="ACHOUK_002381"
     CDS             complement(16848..17483)
                     /gene="thiE"
                     /locus_tag="ACHOUK_002381"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFP9897823.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLSDYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17483..19378)
                     /gene="thiC"
                     /locus_tag="ACHOUK_002382"
     CDS             complement(17483..19378)
                     /gene="thiC"
                     /locus_tag="ACHOUK_002382"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFP9897824.1"
                     /translation="MSATKLTRREQRAQAQHFIDTLEGTAFPNSKRIYITGTQPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGIADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19459..19559)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19611..20087)
                     /gene="rsd"
                     /locus_tag="ACHOUK_002383"
     CDS             complement(19611..20087)
                     /gene="rsd"
                     /locus_tag="ACHOUK_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFP9897825.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            20182..20955
                     /gene="nudC"
                     /locus_tag="ACHOUK_002384"
     CDS             20182..20955
                     /gene="nudC"
                     /locus_tag="ACHOUK_002384"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFP9897826.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRYDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKHLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20995..22059
                     /gene="hemE"
                     /locus_tag="ACHOUK_002385"
     CDS             20995..22059
                     /gene="hemE"
                     /locus_tag="ACHOUK_002385"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFP9897827.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVALYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            22069..22740
                     /gene="nfi"
                     /locus_tag="ACHOUK_002386"
     CDS             22069..22740
                     /gene="nfi"
                     /locus_tag="ACHOUK_002386"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFP9897828.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22783..23373
                     /gene="yjaG"
                     /locus_tag="ACHOUK_002387"
     CDS             22783..23373
                     /gene="yjaG"
                     /locus_tag="ACHOUK_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFP9897829.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREADESNIGIIFQQ"
     gene            23560..23832
                     /gene="hupA"
                     /locus_tag="ACHOUK_002388"
     CDS             23560..23832
                     /gene="hupA"
                     /locus_tag="ACHOUK_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFP9897830.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23845..24540
                     /gene="yjaH"
                     /locus_tag="ACHOUK_002389"
     CDS             23845..24540
                     /gene="yjaH"
                     /locus_tag="ACHOUK_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFP9897831.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24542..24967)
                     /gene="zraP"
                     /locus_tag="ACHOUK_002390"
     CDS             complement(24542..24967)
                     /gene="zraP"
                     /locus_tag="ACHOUK_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFP9897832.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            25205..26581
                     /gene="zraS"
                     /locus_tag="ACHOUK_002391"
     CDS             25205..26581
                     /gene="zraS"
                     /locus_tag="ACHOUK_002391"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFP9897833.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEVWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANCREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASLEGKGARFTLWLPVN
                     ITRKDPQG"
     gene            26578..27903
                     /gene="zraR"
                     /locus_tag="ACHOUK_002392"
     CDS             26578..27903
                     /gene="zraR"
                     /locus_tag="ACHOUK_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFP9897834.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VRERVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHIIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMLVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LAQSLDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27900..29189)
                     /gene="purD"
                     /locus_tag="ACHOUK_002393"
     CDS             complement(27900..29189)
                     /gene="purD"
                     /locus_tag="ACHOUK_002393"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFP9897835.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPTLQ
                     NVAIGVTDIPALLDFAQNEKVDLTIVGPEAPLVKGVVDTFRAAGMKIFGPTAGAAQLE
                     GSKAFTKDFLARHNIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDDVHQRTMERIIWPTVKGMASEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVEDGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(29201..30790)
                     /gene="purH"
                     /locus_tag="ACHOUK_002394"
     CDS             complement(29201..30790)
                     /gene="purH"
                     /locus_tag="ACHOUK_002394"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFP9897836.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTKRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7321 a         8074 c         8537 g         7252 t
ORIGIN      
        1 tggattccta cattccggaa ccagagcgtg cgattgacaa gccgttcctg ctgccgatcg
       61 aagacgtatt ctccatctcc ggtcgtggta ccgttgttac cggtcgtgta gaacgcggta
      121 tcatcaaagt tggtgaagaa gttgaaatcg ttggtatcaa agagactcag aagtctacct
      181 gtactggcgt tgaaatgttc cgcaaactgc tggacgaagg ccgtgctggt gagaacgtag
      241 gtgttctgct gcgtggtatc aaacgtgaag aaatcgaacg tggtcaggta ctggctaagc
      301 cgggcaccat caagccgcac accaagttcg aatctgaagt gtacattctg tccaaagatg
      361 aaggcggccg tcatactccg ttcttcaaag gctaccgtcc gcagttctac ttccgtacta
      421 ctgacgtgac tggtaccatc gaactgccgg aaggcgtaga gatggtaatg ccgggcgaca
      481 acatcaaaat ggttgttacc ctgatccacc cgatcgcgat ggacgacggt ctgcgtttcg
      541 caatccgtga aggcggccgt accgttggcg cgggcgttgt agcaaaagtt ctgagctaat
      601 tgccgataac atttgacgca atgcgcacta aaagggcatc atttgatgcc ctttttgcac
      661 gctttcgtac cagaacctgg ctcatcagtg attttctttg tcataatcat tgctgagaca
      721 ggctctgttg agggcgtata atccgaaaag ctaatacgcg tttcgatttg gtttgcctcg
      781 cgatcgcggg gtgaaaatgt ttgtagaaaa cttctgacag gttggtttat gagtgcgaat
      841 accgaagctc aaggaagcgg gcgcggcctg gaagcgatga agtgggtcgt tgtggtggca
      901 ttgctcttgg tggcgattgt cggcaactat ctttatcgcg acattatgct gccgctgcgt
      961 gcgctggccg tagtaattct gattgctgca gcgggtggtg tcgcgctgtt aacgacaaaa
     1021 ggtaaagcta ccgttgcttt tgcccgtgaa gcgcgtaccg aagtccgtaa ggtcatttgg
     1081 ccgactcgcc aggaaacatt gcacaccacg ctgattgtgg ctgcggttac cgcagtaatg
     1141 tcactgatcc tgtggggact ggatggtatt ctggttcgcc tggtatcctt tatcactggc
     1201 ctgaggttct gagatgtctg aagctcctaa aaagcgctgg tacgtcgttc aggcgttttc
     1261 cggttttgaa ggccgcgtag caacgtcgct gcgtgagcat atcaaattac acaacatgga
     1321 agatttgttt ggtgaagtca tggtaccaac cgaagaagtg gttgaaatcc gtggcggtca
     1381 gcgtcgcaaa agcgaacgta aattcttccc tggctacgtc ctcgttcaga tggtgatgaa
     1441 cgacgcgagc tggcacctgg tgcgcagcgt accgcgtgtg atgggcttca tcggcggtac
     1501 ttccgatcgt cctgcgccaa tcagcgataa agaagtcgat gcgattatga accgcctgca
     1561 gcaggttggt gataagccgc gtccgaaaac gctgtttgaa ccgggtgaaa tggtccgtgt
     1621 taatgatggt ccgttcgctg acttcaacgg tgttgttgaa gaagtggatt acgagaaatc
     1681 tcgtctgaaa gtgtctgttt ctatcttcgg tcgtgcgacc ccggtagagc tggacttcag
     1741 ccaggttgaa aaagcctaac ccagcgatca aaaaagcggc gatttaatcg ttgcacaagg
     1801 cgcgagattg gaatacaatt tcgcgccttt tgtttttatg ggtcttgccc gtaaaacgat
     1861 tttttatatc acggggagcc tctcagaggc gttattaccc aacttgagga atttataatg
     1921 gctaagaaag tacaagccta tgtcaagctg caggttgcag ctggtatggc taacccgagt
     1981 ccgccagtag gtccggctct gggtcagcag ggcgtaaaca tcatggaatt ctgcaaagcg
     2041 ttcaacgcaa aaactgattc catcgaaaaa ggtctgccga ttccggtagt aatcaccgtt
     2101 tacgctgacc gttctttcac tttcgttacc aagactccgc cggcagcagt tctgctgaaa
     2161 aaagcggctg gtatcaagtc tggttccggt aagccgaaca aagacaaagt gggtaaaatt
     2221 tcccgcgctc agctgcagga aatcgcgcag accaaagctg ccgacatgac tggtgccgac
     2281 attgaagcga tgactcgctc catcgaaggt actgcacgtt ccatgggcct ggtagtggag
     2341 gactaagaaa tggctaaact gaccaagcgc atgcgtgtta tccgcgagaa agttgatgca
     2401 accaaacagt acgacatcaa cgaagctatc gctctgctga aagagctggc gactgctaaa
     2461 ttcgtagaaa gcgtggacgt agctgttaac ctcggcatcg acgctcgtaa atctgaccag
     2521 aacgtacgtg gtgcaactgt actgccgcac ggtactggcc gttccgttcg cgtagccgta
     2581 tttacccagg gtgcaaacgc tgaagctgct aaagctgcag gtgctgaact ggtaggtatg
     2641 gaagatctgg ctgaccagat caagaaaggc gaaatgaact ttgacgttgt tattgcttct
     2701 ccggatgcaa tgcgcgttgt tggccagctg ggccaggttc tgggtccgcg cggcctgatg
     2761 ccaaacccga aagtgggtac tgtaacgccg aacgttgctg aagcggttaa aaacgctaaa
     2821 gctggccagg ttcgttaccg taacgacaaa aacggcatca tccacaccac catcggtaaa
     2881 gtggactttg acgctgacaa actgaaagaa aacctggaag ctctgctggt tgcgctgaaa
     2941 aaagcaaaac cgactcaggc gaaaggcgtg tacatcaaga aagttagcat ctccaccacc
     3001 atgggtgcag gtgttgcagt tgaccaggct ggcctgagcg cttctgtaaa ctaatgcctt
     3061 tacgtgggcg gtgattttgt ctacaatctt acccccacgt atgctaacga aagttagcag
     3121 ctaaaaagat ttgttcgttg gagcctggcc tatccaggcc tccgtcgaag accgcaggag
     3181 tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga acgctaagat tattctttta
     3241 tattctggct tgtttctgct caccgtaatt aagacgctct ctccgtttgg aggagtgaag
     3301 tgagttccag agattttctc tggcaaacat ccaggagcaa agctaatggc tttaaatctt
     3361 caagacaaac aagcgattgt tgctgaagtc agcgaagtag ccaaaggcgc gctgtctgca
     3421 gtagttgcgg attcccgtgg cgtaactgta gataaaatga ctgaactgcg taaagcaggt
     3481 cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc tgctgcgccg tgctgttgaa
     3541 ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc cgaccctgat tgcatactct
     3601 atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt tcgcgaaagc gaatgcaaaa
     3661 tttgaggtca aagccgctgc ctttgaaggt gagctgatcc cggcgtctca gatcgaccgc
     3721 ctggcaactc tgccgaccta cgaagaagca attgcacgcc tgatggcaac catgaaagaa
     3781 gcttcggctg gcaaactggt tcgtactctg gctgctgtac gcgatgcgaa agaagctgct
     3841 taatcgcagt tatcttttta acgcattcgc ttacgtataa acttattctg atattcagga
     3901 acaatttaaa tgtctatcac taaagatcaa atcattgaag cagttgcagc tatgtctgta
     3961 atggacgttg tagaactgat ctctgcaatg gaagaaaaat tcggtgtttc cgctgctgct
     4021 gctgtagctg tagctgctgg cccggttgaa gctgctgaag aaaaaactga attcgacgta
     4081 attctgaaag ctgctggcgc taacaaagtt gctgttatca aagcagtacg tggcgcaact
     4141 ggcctgggtc tgaaagaagc taaagacctg gtagaatctg caccggctgc tctgaaagaa
     4201 ggcgtgagca aagacgacgc agaagcactg aaaaaagctc tggaagaagc tggcgctgaa
     4261 gttgaagtta aataagccaa cccttccggt tgcagcctga gaaatcaggc tgatggctgg
     4321 tgacttttta gtcaccagcc tttttgcgct gtaaggcacc agtagcgttt cacactgttt
     4381 gactactgct gtgcctttca atgcttgttt ctatcgacga cttaatatac tgcgacagga
     4441 cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt gatagcaaca ggcattgcgg
     4501 aaagtgttcc attttccggt caacaaaata gtgttgcaca aactgtccgc tcaatggaca
     4561 gatgggtcga cttgtcagcg agctgaggaa ccctatggtt tactcctata ccgagaaaaa
     4621 acgtattcgt aaggattttg gtaaacgtcc acaagttctg gatgtacctt atctcctttc
     4681 tatccagctt gactcgtttc agaaatttat cgagcaagat cctgaagggc agtatggtct
     4741 ggaagctgct ttccgttccg tattcccgat tcagagctac agcggtaatt ccgagctgca
     4801 atacgtcagc taccgccttg gcgaaccggt gtttgacgtc caggaatgtc aaatccgtgg
     4861 cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg gtgatctatg agcgcgaagc
     4921 gccggaaggc accgtaaaag acattaaaga acaagaagtc tacatgggcg aaattccgct
     4981 catgacagac aacggtacct ttgttatcaa cggtactgag cgtgttatcg tttcccagct
     5041 gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt aaaacccact cttcgggtaa
     5101 agtgctgtat aacgcgcgca tcatccctta ccgtggttcc tggctggact tcgaattcga
     5161 tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt aaactgcctg cgaccatcat
     5221 tctgcgtgcc ctgaactaca ccacagagca gatcctcgac ctgttctttg aaaaagttat
     5281 ctttgaaatc cgtgataaca agctgcagat ggaactggtg ccggaacgcc tgcgtggtga
     5341 aaccgcatcc tttgacatcg aagctaacgg taaagtgtac gtagaaaaag gccgccgtat
     5401 cactgcgcgc cacattcgcc agctggaaaa agacgacgtc aaactgatcg aagtcccggt
     5461 tgagtacatc gcaggtaaag tggttgctaa agactatatt gatgagtcta ccggcgagct
     5521 gatctgcgca gcgaacatgg agctgagcct ggatctgctg gctaagctga gccagtctgg
     5581 tcacaagcgt atcgaaacgc tgttcaccaa tgatctggat cacggcccgt atatctctga
     5641 aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca ctggtagaaa tctaccgcat
     5701 gatgcgccct ggcgagccgc cgactcgtga agcagcggaa agcctgttcg agaacctgtt
     5761 cttctccgaa gaccgttatg acctgtctgc ggttggtcgt atgaagttca accgttctct
     5821 gctgcgcgaa gaaatcgaag gttctggtat cctgagcaaa gacgacatca ttgatgttat
     5881 gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc gatgatatcg accacctcgg
     5941 caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac cagttccgcg ttggcctggt
     6001 acgtgtagag cgtgcggtga aagagcgtct gtctctgggc gatctggata ccctgatgcc
     6061 tcaggatatg atcaacgcca agccgatttc cgcagcagtg aaagagttct tcggttccag
     6121 ccagctgtct cagtttatgg accagaacaa cccgctgtct gagattacgc acaaacgtcg
     6181 tatctccgca ctcggcccag gcggtctgac ccgtgaacgt gcaggcttcg aagttcgaga
     6241 cgtacacccg actcactacg gtcgcgtatg tccaatcgaa acccctgaag gtccgaacat
     6301 cggtctgatc aactctctgt ccgtgtacgc acagactaac gaatacggct tccttgagac
     6361 tccgtatcgt aaagtgactg acggtgttgt aactgacgaa attcactacc tgtctgctat
     6421 cgaagaaggc aactacgtta tcgcccaggc gaactccaac ctggatgaag aaggccactt
     6481 cgtagaagac ctggtaacct gccgtagcaa aggcgaatcc agcttgttca gccgtgacca
     6541 ggttgactac atggacgtat ccacccagca ggtggtatcc gtcggtgcgt ccctgatccc
     6601 gttcctggaa cacgatgacg ccaaccgtgc attgatgggt gcgaacatgc aacgtcaggc
     6661 cgttccgact ctgcgtgctg ataagccgct ggttggtact ggtatggaac gtgctgttgc
     6721 cgttgactcc ggtgtaactg cggttgctaa acgtggtggt gtcgttcagt acgtggatgc
     6781 ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat ccgggtgaag caggtatcga
     6841 catctacaac ctgaccaaat acacccgttc taaccagaac acctgtatta accagatgcc
     6901 gtgtgtgtct ctgggtgaac cggttgaacg tggcgacgtg ctggcagacg gtccgtccac
     6961 cgacctcggt gaactggcgc ttggtcagaa catgcgcgta gcgttcatgc cgtggaatgg
     7021 ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt gttcaggaag accgtttcac
     7081 caccatccac attcaggaac tggcgtgtgt gtcccgtgac accaagctgg ggccagaaga
     7141 gatcaccgct gacatcccga acgtgggtga agctgcgctc tccaaactgg atgaatccgg
     7201 tatcgtttat attggtgcgg aagtgaccgg tggcgacatt ctggttggta aggttacgcc
     7261 gaaaggtgaa actcagctga ccccagaaga aaaactgctg cgtgcgatct tcggtgagaa
     7321 agcgtctgac gttaaagact cttctctgcg cgtaccaaac ggtgtatccg gtacggttat
     7381 cgacgttcag gtctttactc gcgatggcgt agaaaaagac aaacgtgcgc tggaaatcga
     7441 agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa gaactgcaga tcctcgaagc
     7501 gggtctgttc agccgtatcc gtgctgtgct ggtagccggt ggcgttgaag ctgagaagct
     7561 cgacaaattg ccgcgcgatc gctggctgga gctgggcctg accgacgaag agaaacaaaa
     7621 tcagctggaa cagctggctg agcagtatga cgaactgaaa cacgagttcg agaagaaact
     7681 cgaagcgaaa cgccgcaaaa tcacccaggg cgacgatctg gcaccgggcg tgctgaagat
     7741 tgttaaggta tatctggcgg ttaaacgccg tatccagcct ggtgacaaga tggcaggtcg
     7801 tcacggtaac aagggtgtaa tttctaagat caacccgatc gaagatatgc cttacgatga
     7861 aaacggtacg ccggtagaca tcgtactgaa cccgctgggc gtaccgtctc gtatgaacat
     7921 cggtcagatc ctcgaaaccc acttgggtat ggctgcgaaa ggtatcggcg acaagatcaa
     7981 cgccatgctg aaacagcagc aggaagtcgc gaaactgcgt gaattcatcc agcgtgcgta
     8041 cgatctgggc gctgacgttc gtcagaaagt tgacctgagt accttcagcg atgaagaagt
     8101 tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc gcaacgccgg tgttcgacgg
     8161 tgcgaaagaa gcagaaatta aagagctgct gaaacttggc gacctgccga cttctggtca
     8221 gatccgcctg tacgacggcc gcactggtga acagttcgaa cgtccggtaa ccgttggtta
     8281 catgtacatg ctgaaactga accacctggt cgacgacaag atgcacgcgc gttccaccgg
     8341 ttcttacagc ctggttactc agcagccgct gggtggtaag gcacagttcg gtggtcagcg
     8401 tttcggggag atggaagtgt gggcgctgga agcatacggc gcagcataca ccctgcagga
     8461 aatgctcacc gttaagtctg atgacgtgaa cggtcgtact aagatgtata aaaacatcgt
     8521 ggacggcaac catcagatgg agccgggcat gccagaatcc ttcaacgtat tgttgaaaga
     8581 gattcgttcg ctgggtatca acatcgaact ggaagacgag taattctcgc tcaaacaggt
     8641 cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa ctccgacggg agcaaatccg
     8701 tgaaagattt attaaagttt ctgaaagcgc agactaaaac cgaagagttt gatgcgatca
     8761 aaattgctct ggcttcgcca gacatgatcc gttcatggtc tttcggtgaa gttaaaaagc
     8821 cggaaaccat caactaccgt acgttcaaac cagaacgtga cggccttttc tgcgcccgta
     8881 tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa gtacaagcgc ctgaaacacc
     8941 gtggcgtcat ctgtgagaag tgtggcgttg aagtgaccca gactaaagta cgccgtgagc
     9001 gtatgggcca catcgaactg gcttccccga ctgcgcacat ctggttcctg aaatcgctgc
     9061 cgtcccgtat cggcctgctg ctcgatatgc cgctgcgcga tatcgaacgc gtactgtact
     9121 ttgaatccta tgtggttatc gaaggcggta tgaccaacct ggagcgtcag cagatcctga
     9181 ctgaagagca gtatctggac gcgctggaag agttcggtga cgaattcgac gcgaaaatgg
     9241 gtgcggaagc aatccaggcc ctgctgaaga gcatggatct ggagcaagag tgcgaacagc
     9301 tgcgtgaaga gctgaacgaa accaactccg aaaccaagcg taaaaagctg accaagcgta
     9361 tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc agagtggatg atcctgaccg
     9421 ttctgccggt actgccgcca gatctgcgtc cgctggttcc gctggatggt ggtcgtttcg
     9481 cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa ccgtaacaac cgtctgaaac
     9541 gtctgctgga tctggctgcg ccggacatca tcgtacgtaa cgaaaaacgt atgctgcagg
     9601 aagcggtaga cgccctgctg gataacggtc gtcgcggtcg tgcgatcacc ggttctaaca
     9661 agcgtcctct gaaatctttg gccgacatga tcaaaggtaa acagggtcgt ttccgtcaga
     9721 acctgctcgg taagcgtgtt gactactccg gtcgttctgt aatcaccgtg ggtccatacc
     9781 tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct ggagctgttc aaaccgttca
     9841 tctacggcaa gctggaactg cgtggtcttg ctaccaccat taaagctgcg aagaaaatgg
     9901 ttgagcgcga agaagctgtc gtttgggata tcctggacga agttatccgc gaacacccgg
     9961 tactgctgaa ccgtgcaccg actctgcacc gtctgggtat ccaggcattt gaaccggtac
    10021 tgatcgaagg taaagctatc cagctgcacc cgctggtttg tgcggcatat aacgccgact
    10081 tcgatggtga ccagatggct gttcacgtac cgctgacgct ggaagcccag ctggaagcgc
    10141 gtgcgctgat gatgtctacc aacaacatcc tgtccccggc gaacggcgaa ccaatcatcg
    10201 ttccgtctca ggacgttgta ctgggtctgt actacatgac ccgtgactgt gttaacgcca
    10261 aaggcgaagg catggtgctg actggcccga aagaagcaga acgtctgtat cgctctggtc
    10321 tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga gtatgaaaaa gatgctaacg
    10381 gtgaattagt agcgaaaacc agcctgaaag acacgactgt tggccgtgcc attctgtgga
    10441 tgattgtacc gaaaggtctg ccttactcca tcgtcaacca ggcgctgggt aaaaaagcaa
    10501 tctccaaaat gctgaacacc tgctaccgca ttctcggtct gaaaccgacc gttatttttg
    10561 cggaccagat catgtacacc ggctttgcat atgcagcgcg ttctggtgca tctgttggta
    10621 tcgatgacat ggtcatcccg gagaagaaac acgaaatcat ctccgaggca gaagcagaag
    10681 ttgctgaaat tcaggagcag ttccagtctg gtctggtaac tgcgggcgaa cgctacaaca
    10741 aagttatcga tatctgggct gcggcgaacg atcgtgtatc caaagcgatg atggataacc
    10801 tgcaaactga aaccgttatt aaccgtgacg gtcaggaaga gaagcaggtt tccttcaaca
    10861 gcatctacat gatggccgac tccggtgcgc gtggttctgc ggcacagatt cgtcagcttg
    10921 ctggtatgcg tggtctgatg gcgaagccgg atggctccat catcgaaacg ccaatcaccg
    10981 cgaacttccg tgaaggtctg aacgtactcc agtacttcat ctccacccac ggtgctcgta
    11041 aaggtctggc ggataccgca ctgaaaactg cgaactccgg ttacctgact cgtcgtctgg
    11101 ttgacgtggc gcaggacctg gtggttaccg aagacgattg tggtacccat gaaggtatca
    11161 tgatgactcc ggttatcgag ggtggtgacg ttaaagagcc gctgcgcgat cgcgtattgg
    11221 gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc tgatatcctc gttccgcgca
    11281 acacgctgct gcacgaacag tggtgtgacc tgctggaaga gaactctgtc gacgcggtta
    11341 aagtacgttc tgttgtatct tgtgacaccg actttggtgt atgtgcgcac tgctacggtc
    11401 gtgacctggc gcgtggccac atcatcaaca agggtgaagc aatcggtgtt atcgcggcac
    11461 agtccatcgg tgaaccgggt acacagctga ccatgcgtac gttccacatc ggtggtgcgg
    11521 catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa caaaggtagc atcaagctca
    11581 gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt tatcacttcc cgtaacaccg
    11641 aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag ctacaaagta ccttacggtg
    11701 cggtactggc aaaaggcgat ggcgaacagg ttgctggcgg cgaaaccgtt gcaaactggg
    11761 acccgcacac catgccggtt atcaccgaag taagcggttt tgtacgcttt actgacatga
    11821 tcgacggcca gaccattact cgtcagaccg acgaactgac cggtctgtct tcgctggtgg
    11881 ttctggattc cgcagaacgt accgcaggtg gtaaagatct gcgtccggca ctgaaaatcg
    11941 ttgatgctca gggtaacgac gttctgatcc caggtaccga tatgcctgcg cagtacttcc
    12001 tgccgggtaa agcgattgtt cagctggaag atggcgtaca gatcagctct ggtgacaccc
    12061 tggcgcgtat tccgcaggaa tccggcggta ccaaggacat caccggtggt ctgccacgcg
    12121 ttgcggacct gttcgaagca cgtcgtccga aagagccggc aatcctggct gaaatcagcg
    12181 gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg tctggttatc accccggtag
    12241 acggtagcga tccgtacgaa gagatgattc cgaaatggcg tcagctcaac gtgttcgaag
    12301 gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc ggaagcgccg cacgacattc
    12361 tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt taacgaagta caggacgtat
    12421 accgtctgca gggcgttaag attaacgata aacacatcga agttatcgtt cgtcagatgc
    12481 tgcgtaaagc taccatcgtt aacgcgggca gctccgactt cctggaaggc gaacaggttg
    12541 aatactctcg cgtcaagatc gcaaaccgcg aactggaagc gaacggcaaa gtgggtgcaa
    12601 cttactcccg cgatctgctg ggtatcacca aagcgtctct ggcaaccgag tccttcatct
    12661 ccgcggcatc gttccaggag accactcgcg tgctgaccga agcagccgtt gcgggcaaac
    12721 gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg tcgtctgatc ccggcaggta
    12781 ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc tgcgggtgaa gctccggctg
    12841 caccgcaggt gactgcagaa gacgcatctg ccagcctggc agaactgctg aacgcaggtc
    12901 tgggcggttc tgataacgag taatcgttaa tccgcaaata acgtaaaaac ccgcttcggc
    12961 gggttttttt atggggggag tttagggaaa gagcatttgt cagaatattt aaggaatttc
    13021 tgaatactca taatcaatgt agagatgact aatatcctga aactgactga actaattgag
    13081 tcaaactcgg caaggattcg atactattcc tgtgtagctt tcttaaggaa cgagaatgaa
    13141 acaggaagtg gagaagtggc gaccttttaa acatccggat ggcgacgttc gtgatttatc
    13201 gtttcttgat gctcatcagg ttatctacgt tcagcatcat gaggacaagg agcctttaga
    13261 gtatcgcttt tgggttacct actctcttca ctgcttcaca aaagattatg aacatcagac
    13321 gaacgaagaa aaactatcgt taatgtacca cgcgcctaaa gaatctcgtc ccttctgcca
    13381 gcaccgttat aacttagcgc gcacacactt aaaaagaact attttggcgc tgccagaaag
    13441 caacgttatt catgccgggt atggtagcta tgccgtgatt gaggtggact tagacggagg
    13501 agataaggca ttttactttg ttgtgttcag ggctttcagt gaaatttttc accattgcct
    13561 aaaacttatt gagaaataag cagcttcctc agccctcaaa ataacaaaac ccaccttaag
    13621 gtgggtttcg ccagagaatt atctctggta ttcagaacgc cattaccgga ctttgccttg
    13681 accttgcgat aatcgcaggt tgcgggatgt ctgaatttct tcagtctgct gcatcctgga
    13741 agatgagaac atgtgttctt attttcgtct ctatcatagt tgagtattta ctctcttaca
    13801 atcagatctc tttcattgct caacaggcga tggcttcaga ctttgcatta cggaattttt
    13861 aagaaaggca gggcgaaacg aggaagaagc ttttcgccct ggacaacatt cctgctgacg
    13921 gcactaccat aaaaaaacac aacgctttca acaaccttgc gtttttcatc ttccgtagcg
    13981 gctcggaata aattttcgtt cttacattcc gtctcatagt ctttgcgagg cgcgtcccag
    14041 atagctgtcc cagtctttcc ataccggctg caaaccctga gcggttaacg cggcagcaac
    14101 cgcttccggt ctgcgctcgt cgtgcggtga gaactgttcc agctcggggt gattgtcggc
    14161 atagccacct ggctgcgttt tcgaaaaagc gctgacgtta ttaattgcca gcggaataac
    14221 gcgatcgcga aaccacggtg attcccgcgt ggagagtgac agttcaatct ccggtgcaag
    14281 cagccggaag gcgcagatgg tttgcactaa ctggcgttca tccataatcg acgcaggctc
    14341 aatgccgcca gtacatggac gcaggcgtgg gaaggagaca gagtagcggc tttgccagta
    14401 atgctgttgc agccatagca aatgttctgc aaccatatag cagtcaacgc gccagctgtc
    14461 ggaaaggcca attagcgcgc cgaggcctat cttatcaatc cccgcacggc ccagccgatc
    14521 cggcgtttcc agccgccaga agaagtcctg ttttttgcct ttcagatgat ggcgggcata
    14581 agtcgcctcg tgatatgtct cctgataaac catcacgcca tccagaccaa gttgctttaa
    14641 ctcggcgtat tccgtctccg ccagcggctg tacttccatc tgtagtgaag agaactgttc
    14701 acgcagggca gggagatgac gacgaaagta atccatcccc actttcgcct gatgttcacc
    14761 ggtgactaaa agcagatgtt caaagcccat ctcccgtata gcggcgcttt ccctggcaat
    14821 atccgcttca tccagcgttt tgcgcttgat gcggttgctc atagagaatc cgcagtacgt
    14881 gcagtcgtta gcgcaaagat tggaaagata aagcgggacg tagaaactaa ctgtgttgcc
    14941 aaatcgctga cgggtcagac gctgcgcccg ttgggccagt tgttccagat agccactggc
    15001 ggctggcgat aacagcgcca tcatatcgtc gcgggtgagt tgcgaggcat ttagcgcccg
    15061 ctctacgtca gcagccgttt tgccgttgat acgcaggcgg atgtcgtccc agtccagttg
    15121 tcgccagcga tcgctgaagg ttttcatacc gatgcctcca gaaatccggt cagcgggctg
    15181 gtggcatgag caaaataact gcggctgccc ggtccggact gaggtgccag caggcctgct
    15241 tctactgcca gacgaaatgc cttcgccatg ttgacgggat cgtccgcgac ggcaatcgcc
    15301 gtattcacta acaccgcgtc ggcccccatt tccagcgcct gcgcggcatg gctgggaacg
    15361 ccgatgccag catcgacaac caccggaact gtggcctgct ggataataat ctccagcatg
    15421 gcgcgggttt ccagcccctg attcgagcca atcggcgcgc cgagcggcat caccgctgca
    15481 cagccgactt cttccagacg tttacacaat accggatcgg ccccgcagta aggcagcacg
    15541 acaaatccct gttgtaccag cgtttcggcg gctttcaggg tttcgatggg atcgggcaac
    15601 agccagcggg cgtcagggtg aatctccaat tttaaccagt ttgtgcctaa cgcttcacga
    15661 gccagatggg cggcgaaaat ggcttcttcc gctgttttcg ccccggatgt atttggcagc
    15721 agggtcacac ccgccgcgat aagcggttcg aggatagcgt cgttgtgctg gcgcaagtcg
    15781 acacgtttca tcgccagcgt caccagctgg ctgccggaag cgcggattgc ctctaccatc
    15841 agttgtgaag aggcgaattt cccggtgccg gtaaacagat gtgaatcaaa cgttttgtcc
    15901 gcaatacgta acatctcaac cccctgcaat aacctgaaaa agtaggatct ggtcgccatc
    15961 ctgcacgata tgttgcgtcc actgctcacg cgggacgatt tgctgattaa tcgccagagc
    16021 cgcgcccgct tgtcgttgaa cgagttgctc cagtagttcg tgaacagttt gcccggcggc
    16081 gcactgcatc ggttgatcgt taaacaggat ctgcattgct tcctccgcat accgggcaac
    16141 cactggcgcg gcgcaacgcc aggctgcgcc actggctcga cttaccgtcg aacagtcgga
    16201 gttctcccgc aggcgtctct ataccgctta ataacttaat ggcttccagt gcctgcaaag
    16261 tgcccataac cccgaccacc gggccaacca cgcccgccgt gcggcagttg cgttctggct
    16321 cctggttttc tggccacagg cagcggtaac aaccctgccc ccagggcggc gtcagtacca
    16381 tcaactgacc gccaaatccg accgcgctgg cggtgataag cggcgtgttg agtgccacgc
    16441 aggcggcatt aatctcctgg cgagtcgcca tattgtcggt acagtcgagc accacatcgg
    16501 cttgtgcaac cgcatctttt aacgcctcac ccgttaaccg ttgttgtaat gctgtcagtt
    16561 gaatatcggg attcaactgt gtcagtcgct gatggctgac ctgcgatttc gggcgatcga
    16621 tatcttcagt ggtaaagagg atttgtcgtt gcagattgct taaatgcaca tcgtcgtcat
    16681 ctgccagtac cagcgtcccg acgccagcgc ccgccaggta tagcgcagca ggtgtaccca
    16741 gcccgccaag accgataatc agcacctggc tgtcgagcag tttttgctgc ccgtcaagag
    16801 cgatatcgtc gagcaggatt tggcggctat aacgcataaa atcacggtca ttcatcgcca
    16861 actcctgcaa tttccagcaa ctgtgccgtt gccaaacgcc agtctgcggc ttgagtaatg
    16921 gcgctgacga cggcgatact gccgacaccc gttgctatca ccgcaggcgc gcgtgccaga
    16981 ctgataccgc caatcgccac ggtgggataa tccgacagtc gctcaacatg ccgtgccagc
    17041 tgttccagcc cctgcggtgc agaaggcatc tgtttggttt gcgtcgggaa cacatgtccc
    17101 agcgcgatat aagaggggcg tgctgccagc gcgacgtcga tttccatatc gtcatgggtc
    17161 gaaacgccca gccgcaggcc tgccgcgcgg atggcattga gatcggtggc ttgcaaatct
    17221 tcctgcccca aatggacgcc atacgcctga tgcttgatcg cgagccgcca gtaatcgttg
    17281 ataaacaatc gcgcgttata gcggcggccc agcgcaattg ccgccacgac atcggcttcc
    17341 acctcttcat cgcgccgatc tttgatgcgt agctggagag tacgtacgcc tgcatccaac
    17401 agacgttcga tccactgtac gctgtccacc accgggtaca gtcctaaacg aaaaggtaca
    17461 ggaggaaaat caggctgata catcacgctt cctccttacg caggtagatt tctccgcctc
    17521 tggcacggaa gttctccgac atatccgcta ttcccatttc aatagtttgc gtggcggcgt
    17581 aatcacgcac ttcctggctg attttcatcg agcagaattt cggcccacac atggagcaaa
    17641 aatgggcgac tttacctgac tcttgcggca gggtttcatc gtgataagcg cgggcggtaa
    17701 acgggtcgag ggccagatta aactggtctt cccagcgaaa ttcgaagcgg gctttcgaca
    17761 tggcgttatc gcgaatttgt gcgcccggat gccctttcgc caggtcagcg gcgtgggcgg
    17821 caatcttata ggtgataagc ccctgcttaa catcttcttt attaggcaga cccagatgct
    17881 cttttggcgt tacgtaacag agcatcgcgc agccaaacca gccaatcatc gccgcaccaa
    17941 tccccgacgt gaagtggtca tagcccggcg caatatcggt agttagcggc cccagagtgt
    18001 aaaacggcgc ttcgtggcag tgctctaact cctcggtcat attgcggcgg atcatctgca
    18061 tcggcacgtg gcctgggcct tcaatcatca cctgcacatc atattcccag gcgatttttg
    18121 tcagttcgcc cagcgtatgc agctcggcaa actgcgcttc atcgttggcg tcctgaatag
    18181 aaccggggcg cagaccgtcg cccagcgaca gcgaaacgtc ataagcggca cagatttcac
    18241 aaatttcgcg gaagtgttga tagaggaaat tttcctgatg atgggagagg caccatttcg
    18301 ccataatcga accgccgcgc gagacgatac cggtcaggcg tttcgcggtc atcggcacat
    18361 agcgcagcag cacccccgca tggatagtga agtaatccac accttgctcg gcctgttcca
    18421 gcagcgtgtc gcggaacgct tcccaggtaa gatcttcggc gatcccatta accttctcca
    18481 gcgcctggta gatcggcact gtaccaatcg gcaccgggct gttacgcaaa atccactcgc
    18541 gggtttcgtg aatatagcga ccggtggaga gatccatcac cgtatccgct ccccagcgcg
    18601 tggaccatac cagcttttcc acttcttctt cgatggaaga ggtgaccgcc gagttgccga
    18661 tattggcgtt aacttttacc aggaaattgc gaccaataat catcggctcc gattccggat
    18721 gattaatgtt ggccgggata atcgcacgtc cggcagcaac ttcatcacgg acaaattccg
    18781 cagtgatatt ttccggcaga cgtgcgccaa agctcattcc cggatgctgg tggcgtaaaa
    18841 cttcgctacg gatgcgctcg cggcccatat tctcgcggat ggcgatgaat tccatttccg
    18901 gtgtgatgat gccctggcgg gcgtagtgca gttgtgtgac gcggcgtcct gctttggcgc
    18961 gttttggcgt taatacgccg ctaaaacgca gctcgtcgag gccatcatct gccagccgcg
    19021 ctttagtgta atcggaactg cgcacggtaa gttcttcggt atcgccgcgc gcatcgatcc
    19081 acggctggcg tagttttgcc agcccttgct gcacgttaat ggcgatctgc ggatcgccat
    19141 acgggccgga ggtgtcgtag accggaatcg cttcgttttc ttcgtactgc ggctgttctt
    19201 tgctaccgcc aattagcgtc gggctaagct ggatctcacg catcggcacg cgcacgccgg
    19261 gttgtgtgcc agtgatataa atgcgttttg agttgggaaa ggcggtgcct tccagggtgt
    19321 cgataaaatg ttgggcctgg gcgcgttgtt cgcgacgggt cagttttgtt gcagacatag
    19381 ctcattccaa aaagttaagg acgtggcttg tcagacgacg gatgaagcaa gagacgatcg
    19441 ccgcaaaggc gatgcgatag cagattaact cttgttccct tcgcaggtat tagcctgatc
    19501 aggttccgcg gatcccgaat aaacggtctc agccagttaa ggcactccga caagaaatta
    19561 gccccacaaa cggggcgttg aatgtaaatt acgcgttaac agcgcagaac tcaagcagga
    19621 tgtttgacgc gggcggcgtc aagcaccagc agaatcagct tatcttccag cacaaagcgc
    19681 gcttccagcg cctcgccgat gtcagataaa acctgttgaa actcaaggta attatcatga
    19741 tcgatagcgg tttccaggct ggaatcgtag taatccataa tctgttgggt attggcttcg
    19801 agttgcggcc aaatcttcgc ggcgcgcgcg agttgcccgt tgccttccag cttatgaaga
    19861 atgcgctcat aaatactgaa atgtccggca gacaagtaat cgaccaggct ctgacaaaaa
    19921 tcatcaaggg ctttttcgtt tagcctcatg tacgattctt tgccaggctt aatgccaacc
    19981 agattgtagt aagccacgag cagatgctta cgtacatgta gccagcgatc aaccagtttg
    20041 ttacttcctc tgacgcgctc cgtcaggtta tcgagctggt taagcatgat tgactccgca
    20101 agtttgtatt caaaaactgc tcagtgagaa atgtaaaaac catgttaaac atgccagtga
    20161 tgcaaaggta gtgcaagagc tatggatcgt ataattgaaa aattagatca cggctggtgg
    20221 gtcgtcagcc atgaacaaaa attatggttg ccgaagggag aattgccata tggcgaagcg
    20281 gcaaatttcg atcttgtggg tcagcgcgca ctacaaatcg gcgaatggca gggggaacct
    20341 gtttggttaa tacaacagca gcggcgttac gatatggggt cggtacgtca ggtcattgat
    20401 ctcgatgttg ggctgtttca actggccgga cgtggcgtac aactggcgga gttttaccga
    20461 tcgcataaat actgtggtta ctgcgggcat gaaatgtatc caagcaaaac cgaatgggcg
    20521 atgctgtgca gccattgccg tgagcgttac tacccgcaaa tcgccccctg cattattgtt
    20581 gccatccgtc gcgatgattc gatcctcctc gcccagcata cccgccatcg taacggtgtc
    20641 catacggtac ttgccggatt cgtcgaagtg ggcgaaaccc tcgagcaggc ggtcgcgcgg
    20701 gaagtgatgg aagagagcgg aattaaagtt aaacacttgc gttatgtgac ttcccagccg
    20761 tggccgtttc ctcagtcttt aatgaccgcg tttatggcgg aatatgacag cggcgacatc
    20821 gtgatcgacc cgaaagaatt gctcgaggcg aactggtatc gctatgacga tttgccgtta
    20881 ctcccgccgc ccggcaccgt agcgcgccgt ctgatagaag atacggtggc gatgtgtcgg
    20941 gcagagtatg agtgatgata cactgaccgc ctgacgcact aaggaacagc caaaatgacc
    21001 gaacttaaaa acgatcgtta tctgcgggcg ctgctgcgcc agcccgttga tgtcactcct
    21061 gtatggatga tgcgccaggc gggtcgctat ctaccggaat ataaagccac gcgcgcccag
    21121 gcgggcgatt ttatgtcgct gtgcaaaaac gccgagctgg cgtgtgaagt gaccttgcaa
    21181 ccgctgcgtc gctacccgct ggatgcggcg atcctctttt ccgatatcct caccgtcccg
    21241 gacgcgatgg ggttagggct ctattttgaa gccggagaag gtccgcgttt tacctcgcca
    21301 gtcacctgca aagctgacgt cgataaactg ccaattccgg acccggaaga tgaactgggg
    21361 tacgtgatga acgcggtgcg taccattcgt cgcgaactga aaggcgaagt gccgctgatt
    21421 ggtttttccg gcagcccgtg gacgctggcg acctacatgg tggaaggcgg cagcagcaaa
    21481 gccttcaccg tgatcaaaaa aatgatgtat gccgatccgc aggcgctgca cgctctactc
    21541 gataaactgg cgaaaagcgt cgctttgtat ctgaatgcgc agattaaagc cggtgctcag
    21601 gctgtgatga ttttcgacac ctggggcggc gtgcttaccg ggcgcgatta tcaacagttc
    21661 tcgctttatt acatgcataa aattgttgat ggtttactgc gtgaaaacga cggtcgccgc
    21721 gtaccggtca cgctgtttac caaaggcggc ggacagtggc tggaagccat ggcagaaacc
    21781 ggttgcgatg cgctgggcct cgactggaca acggacatcg ccgatgcgcg ccgccgtgtg
    21841 ggcaataaag tcgcgttgca gggtaatatg gatccgtcga tgctgtacgc gccgcctgcc
    21901 cgcattgaag aagaagtagc gactatactt gcaggtttcg gtcacggcga aggtcatgtc
    21961 tttaaccttg gtcacggcat tcatcaggat gtgccgccag aacatgctgg cgtgttcgtg
    22021 gaggcagtgc atcgactgtc tgaacagtat caccgctaag gagcgattat ggatctcgcg
    22081 tcattacgcg ctcaacaaat cgaactggct tcttctgtga tccgcgagga tcgactcgat
    22141 aaggatccgc cggatctgat cgccggagcc gatgtcgggt ttgagcaggg cggagaagtg
    22201 acgcgagcgg cgatggtgct gctgaaatac ccctcgcttg agctggtgga gtataaagtg
    22261 gcccgcatcg ctaccaccat gccttacatt ccaggttttc tttccttccg cgaatatcct
    22321 gcgctgctgg cagcgtggga gatgctgtca caaaagccgg atttagtgtt tgtcgatggt
    22381 catgggatct cgcatcctcg ccgtcttggc gtcgccagcc attttggttt gctggtggat
    22441 gtgccgacca ttggcgtagc gaaaaaacga ctatgcggta aattcgaacc gctctccagc
    22501 gaaccgggcg cgctggcccc actgatggat aaaggcgagc agctggcctg ggtctggcgc
    22561 agcaaagcgc gctgtaaccc gctatttatc gctactggtc atcgggtcag cgtggacagc
    22621 gcgctggcgt gggtacaacg ctgcatgaaa ggctatcgtc tgccggagcc aacgcgctgg
    22681 gcggacgcgg tggcctcgga acgtccggcg ttcgtgcgct atacagcaaa tcagccctaa
    22741 ttcaggtaaa ctgcggataa tttccgtatt tgagaactca acatgttaca aaacccaatt
    22801 catctgcgtc tggaacgcct ggaaagctgg cagcatgtca ctttcatggc ttgcttatgc
    22861 gaacgcatgt accccaatta cgccatgttc tgccagcaaa ccggttttgg tgatgggcaa
    22921 atttaccgtc gtattctcga tctcatctgg gaaacgctga ccgttaaaga tgcaaaagta
    22981 aatttcgaca gccaactgga gaaatttgaa gaagcgattc cttcagccga cgatttcgat
    23041 ctgtacggcg tttatccggc aatcgatgcc tgcgtggcgt taagtgaact ggtccattcg
    23101 cgtttgagtg gtgaaacgct cgaacacgcg gtggaagtga gtaagacctc catcacgacc
    23161 gttgcgatgc tggaaatgac tcaggctggt cgcgaaatga gcgatgaaga gctcaaagaa
    23221 aacccagctg tagagcaaga atgggacatt cagtgggaaa tattccgact tttagctgag
    23281 tgcgaagaac gcgatatcga gctgataaaa ggccttaggg cagacctgcg tgaggcggat
    23341 gagagcaata ttggtataat ttttcagcaa taagaccaga aaacgtgatt taacgcctga
    23401 tttgtcgtac ctggagtctt ccctttcgcc ccccgtctgg tctacatttg gggggcgaaa
    23461 aaaagtggct atcggtgcgt gtatgcagga gagtgctatt ctggcatttc cgtcgcactc
    23521 gatgcttagc aagcgataaa cacattgtaa ggataactta tgaacaagac tcaactgatt
    23581 gatgtaattg cagagaaagc agaactgtcc aaaacccagg ctaaagctgc tctggagtcc
    23641 actctggctg caattactga gtctctgaaa gaaggcgatg ctgtacaact ggttggtttc
    23701 ggtaccttca aagtgaacca ccgcgctgag cgtactggcc gcaacccgca gaccggtaaa
    23761 gaaatcaaaa ttgccgcagc taacgtaccg gcatttgttt ctggcaaggc actgaaagac
    23821 gcagttaagt aagattgcgt ggcagtgaac agttttaacg aaggggtggt ttcacccctt
    23881 ttgtctttct ggcgtcgatc attgatgctg gctggcgctc tgtttctcac tgcttgtagt
    23941 cacaactctt cacttcctcc ctttaccgcc agtggatttg ctgaagacca gggcgcggta
    24001 cgcatctggc gaaaagacag cggcgataat gtgcatcttc ttgccgtgtt tagcccgtgg
    24061 cgcagtggcg ataccacgac gcgagagtat cgctggcagg gcgataacct cacgctcatc
    24121 aatatcaatg tttacagcaa acccccggtg aatattcgcg cgcgttttga cgatcgcggt
    24181 gatctgagct ttatgcaacg tgaatccgat ggggaaaagc agcagctttc taacgaccaa
    24241 atcgatttat accgttatcg ggctgatcag atccgccaga ttagcgatgc cttacgtcag
    24301 gggagagtcg tgctgcgcca ggggcgctgg catgcgatgg aacagaccgt gaccacctgc
    24361 gaagggcaaa ccattaaacc tgatttagat tcgcaggcaa tagcgcatat cgagcgccgc
    24421 cagagccgct cttctgttga tgtcagcgtg gcatggctgg aagcgcccga aggttcgcaa
    24481 ttactgttag tggcaaactc tgatttctgt cgctggcaac ccaacgagaa aacgttctga
    24541 tttaccagtg gcccataccc atatgaccgc caccaccgca gccgccgtag cccatgccca
    24601 ttccggctcc gcgcggaata cccgcttcag ccatcgcaat atcccgtttc acccgtaact
    24661 catctaacga ctgacgcaaa ttctccatct ctttggcgac cgcgttaatt ttgctgctat
    24721 ccggtgggtt cgcggctaac agggcattgt attcataacg cttcgtcacc agttgctgtt
    24781 gcagtgcgct gctttgagcg taaaagtcat tatggatttt ctgccacgct gtctgttgtt
    24841 cgctggtcaa aggcgcggca ttttgctgcc acataccgtg tccgccgtga gcaaatgcag
    24901 atgtcgatcc catcgccatt gctgaaagcg ccatcattac cagggcaatt ttcgtgttcc
    24961 gtttcatggt taatcctcca gtggttgtct tacttcgggt attgcatctt ccgtgccaac
    25021 gatgaaacgc tgatatgacg ggtaatctgg catgataaac gagtaaaaat gactcgcctg
    25081 ctgcgggtag cgagtcattt ttactcattg aaacttaagc ctttgtgtta cagcgcaggg
    25141 taagcgctga taaaagatgg catgatttct gctgtcagaa agggatgagc aggcaaagaa
    25201 gaagatgcgt tttatgcaac gttctaaaga ctccttagct aaatggttaa gcgcgatcct
    25261 ccccgtggtc attgttgggc tggtagggtt gtttgcggtg acggtgattc gcgattatgg
    25321 ccgcgagact gccgccgcca gacaaacgct gctggaaaaa ggcagtgtac ttattcgtgc
    25381 tctcgaatcc ggctcgcgcg tcggcatggg gatgcgcatg catcatgcgc agcagcaggc
    25441 cttactggaa gaaatggccg ggcagcctgg agtacgttgg tttgcggtca cggatgaaca
    25501 aggaacaatc gtgatgcata gcaactccgg catggtggga aaacagcttt attccccgca
    25561 ggaaatgcag cagttacatc cgggagatga agaagtgtgg cggcggatcg atagcgcaga
    25621 cggcgagcct gttctggaaa tttatcgcca gtttcaaccg atgtttgctg ctggaatgca
    25681 ccggatgcgc catatgcagc agtatgccgc gacaccacaa gcaattttca tcgctttcga
    25741 cgccagtaat attgtgagtg ccgaagatcg tgagcagaga aacaccctga ttatcctctt
    25801 cgccctggcg acggtcttgc tggcaagcgt attgtcattc ttctggtatc gccgctatct
    25861 gcgctcgcgc cagcttctac aagatgaaat gaagcgcaaa gagaagctgg tggcgctggg
    25921 gcatcttgcg gcaggcgttg cccacgaaat tcgtaacccg ctttcctcga ttaaagggct
    25981 ggcaaaatac tttgccgagc gcgcgccagc agggggagaa gcgcatcaac tggcgcaggt
    26041 gatggcgaaa gaagctgacc gtttaaaccg cgtggtaagc gagctgctgg aactggttaa
    26101 gccaacgcat ctggctttgc aggcggtgga tctcaacacg ctgattaacc actcattaca
    26161 gctggtaagc caggatgcaa actgccggga gatccagtta cgctttaccg ccaacgacac
    26221 gttgccggaa attcaggccg atccggacag gctgactcag gtcctgttga atctttatct
    26281 caatgctatt caggcgattg gtcagcatgg cgtgattagc gtgacggcca gcgaaagcgg
    26341 cgcgggcgtg aaaatcagcg ttaccgacag cggtaaggga attgcggcag atcagcttga
    26401 agccatcttc accccgtact tcaccaccaa agccgaaggc accggactgg ggctggcggt
    26461 cgtgcataat attgttgaac aacacggtgg tacaattcag gtcgcaagcc tggagggaaa
    26521 aggcgcaagg ttcaccctct ggcttccggt caatattacg cgtaaggacc cacaaggatg
    26581 acgcacgata atatcgatat tctggtggtg gatgatgaca ttagccactg cactattttg
    26641 caggctttgc tgcgcggctg gggctataac gtcgcgctgg cgaacagcgg gcgacaggcg
    26701 ctggagcagg tgcgggagcg ggtttttgat cttgtgcttt gcgatgtgcg aatggcggag
    26761 atggacggca ttgccacgct aaaagagatc aaagcgttaa atccggcgat tccggtactg
    26821 attatgaccg catactctag cgtcgagacg gcggtagagg cgttgaaaac cggggcgctg
    26881 gattatctca tcaagccgct ggatttcgat aacctgcagg cgacgctgga aaaggcgctc
    26941 gcgcatacgc acattattga tgctgaaacg cctgcggtga ctgccagcca gttcggtatg
    27001 gtcggtaaaa gcccggcgat gcaacacctg ctcagtgaaa tcgccctcgt cgcgccatcg
    27061 gaagccacgg tgctgatcca cggcgattcc ggcaccggta aagagctggt cgccagggcg
    27121 attcacgcca gtagcgcacg tagcgaaaaa ccgctggtaa cgctcaactg tgcggcactc
    27181 aacgaatcct tgctggaatc tgaattgttc ggtcacgaaa aaggggcgtt tactggagcc
    27241 gataaacgcc gggaagggcg ctttgttgag gcggacggcg gcacgttgtt tctcgatgaa
    27301 attggcgata tctcgccgat gatgctggtg cgtctgctgc gtgcgattca ggagcgcgaa
    27361 gttcagcgtg tcggtagcaa ccagactatt tcggttgatg tccggctgat tgccgcgacc
    27421 catcgcgatc ttgccgcaga ggtgaatgcc gggcgttttc gccaggatct ctactatcgc
    27481 ctgaacgtgg tggcgattga agtaccatcg ctgcggcaac ggcgggaaga tattcctctg
    27541 ctggctggcc attttctgca gcgctttgct gaacgtaatc gcaaggcggt aaaaggcttt
    27601 acgccccagg cgatggatct gttgattcat tacgactggc cgggaaatat tcgtgaactg
    27661 gaaaatgcgg tggaacgggc ggtggtgctg ctgactgggg aatatatttc cgaacgcgag
    27721 ctgccgctgg cgattgccag tacgccgatc ccgctggcac aaagcctgga tattcagccg
    27781 ctggtggaag tcgaaaaaga agtgattctg gcggcactgg agaaaacggg cggcaacaaa
    27841 accgaagccg cccgtcagtt agggatcacg cgcaaaacgc tgttggcaaa attgtcgcgt
    27901 tagttctgct cgcgttcgat agcgcgccag ccgatatctt tccggcagaa gcagtcgtcc
    27961 cagtggatat cggtcattaa ggcataggcg cgtttctgtg cttctgctac ggtatgaccc
    28021 agcgcggtga cgcacagtac gcgcccgccg ctggttacca cctgctcgtc atccgccagt
    28081 tttgtgcccg cgtggaacac tttgccgtct tccacttctt ccagcggcag gccgtggatc
    28141 acgtcaccgg tgcggtaatc gcccggatat ccacccgcag ccatcaccac gccgagagaa
    28201 gcacgttcat cccactctga cgttttctcg tctagtttgc cttcacaggc cgccaggcag
    28261 agttcaacaa gatcggactt catgcgcagc ataatcggct gggtttctgg atcgccaaag
    28321 cggcagttaa attcgataac cttcggattg ccctgtttgt cgatcatcag gcccgcgtag
    28381 agaaaaccgg tgtaggtgtt gccttccgac gccatgcctt tcacggttgg ccagatgata
    28441 cgttccatcg tgcgctgatg aacgtcatct gtcactaccg gcgcaggaga gtaagcgccc
    28501 attccgcccg tgttcggccc ggtatcttta tcgcctacgc gtttgtgatc ctggctggtg
    28561 gccatcggca gcacatgctc gccgtccacc atcacgataa agctcgcttc ttcgccgtcg
    28621 aggaactctt cgataacgat gcgatgaccc gcgtcgccaa acgcgttgcc cgccagcata
    28681 tcgtgaacag ccgcttccgc ttcttccagc gtcatcgcca cgataacgcc tttcccggca
    28741 gccagaccgt ccgctttaat gacgattggc gcgcctttct cacgcagata cgccagcgca
    28801 ggttctacct cggtgaagtt ctgatattcc gccgtaggaa tgttatggcg ggccaggaaa
    28861 tctttagtaa acgctttcga gccttccagc tgtgcggcgc ctgcggttgg gccgaagatt
    28921 ttcatcccgg cggcgcggaa ggtatcgacc acgcctttca ccagcggcgc ttccgggccg
    28981 acgatggtta gatctacctt ttcgttttgc gcgaaatcca gcagcgccgg gatatcggtc
    29041 acgccgatag cgacgttttg cagcgtgggt tccagcgctg tgcctgcgtt acccggagca
    29101 acaaaaacag tctcaaccag cggcgactgg gccgctttcc aggccagcgc gtgctcgcgc
    29161 ccgccgttac caatcactaa tactttcatc tgtcgctcca ttaatggcgg aagtggcgca
    29221 tatcggtgaa gagcatcgca ataccgtgct cgtcggcggc ggcaatcact tcgtcatcac
    29281 ggatggaacc gccaggctgg attacgcagg tcacgcccgc agcggcggcg gcatcaatac
    29341 cgtcgcggaa cgggaagaac gcgtcagaag ccatcgagga acctttcact tccaggcctt
    29401 catcggcagc tttaataccg gcgattttcg cggagtacac gcggctcatc tggcccgcgc
    29461 caataccgat ggtcatattg tttttggcat agacgatagc attggatttc acgaacttcg
    29521 ccactttcca gcagaacagc gcatcacgca gttcctgttc ggtcggctga cgtttggtga
    29581 cgacgcgcag ttcttccgca ccgaccatgc ccagatcgcg atcctgaacc agcagaccgc
    29641 cgttcacgcg tttgaaatca agacccggaa cacgctcgcc ccactgaccg caggtcagaa
    29701 cgcgtacatt ctgtttggcg gcggtgattt tcagggcttc ttcgctggcg gacggcgcaa
    29761 taatcacttc aacaaactga cgagaaatga tggcctgtgc ggtttccgca tccagctcgc
    29821 ggttaaaggc aatgatgccg ccgaatgcgg aggtcgggtc ggttttgtac gcgcgatcgt
    29881 aagcatcaag aattgaattg ccgatagcca cgccgcaagg gttggcgtgc ttcacaatca
    29941 cacatgccgg ctcggcgaac tctttcacgc actccagcgc cgcatcggta tcggcgatgt
    30001 tgttataaga gagggcttta ccctgaacct gggttgcggt agcaacggag gcttctttca
    30061 cattctcttc tatatagaag gcagcctgct ggtggctgtt ctcgccgtaa cgcatatcct
    30121 gcttcttaat gaagttcagg ttcagcgtgc gtgggaagcg accggcggct tctttgcttt
    30181 caccgtgata agccggaacc atgctgccga agtagttggc aatcatgctg tcgtaggcgg
    30241 cagtgtgttc gaaggctttg atggcgaggt cgaaacgggt tgcaagcgtc agcgatcctt
    30301 cgttgtcatc catctcttta ataatggcgt cgtagtcgct gctcttcacc acgattgcga
    30361 catctttatg gttcttggcg gcggagcgca ccatcgttgg gccgccgata tcgatgttct
    30421 caaccgcatc ttccagcgag caaccttcac gggccacggt ctgggcgaac ggatacaggt
    30481 taacaaccac catatcgata ggctggatct gatgttcttc cataatggca tcgtcctggc
    30541 cgcgacggcc cagaatgcca ccatgtactt tcggatgcag ggtcttcacg cgtccatcca
    30601 tcatctccgg gaaaccggtg taatcggaaa cttcggttac cggcagacct ttttctgcta
    30661 acagacgggc agtgccccct gtagacagca gctccacacc gcgtgcggaa agtgcctggg
    30721 cgaattcgac gataccggct ttgtcagaaa cactgagcag agcgcggcgg actggacgac
    30781 gttgttgcat ggtaaatccc ctggatttga ctattacaga gagcgttagc tgaatttttc
    30841 gcgaaaaact cagctaacgc ccctaacggg gcatccttat ttttcgcccg cattgtaacg
    30901 aaaacgtttg cgcaacgctc gcgaattttt ttaattcgat ggcgatcacg attttgcccc
    30961 cgattagcgt gggagaaata gagaaataac gctgatgatt gtggataact ctgtgcgtaa
    31021 aaaggtataa agcggggttt tgctggggaa tgcagcagtc agtcattttt ctgcaatttt
    31081 tctattgcgg cctgcggaga actccctata atgcgcctcc atcgacacgg cggatgtgaa
    31141 tcacttcaca caaacagccg gttcggttga agagaaaaat tctg
//
LOCUS       DAZKIP010000048        30457 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZKIP010000048 DAZKIP010000000
VERSION     DAZKIP010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.048"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..295)
                     /locus_tag="ACHOUK_002395"
     CDS             complement(<1..295)
                     /locus_tag="ACHOUK_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HFP9897837.1"
                     /translation="MDCSKCNGYATMLLNMVQGSDPVNLLELHGFLEHFAYYVSFGKF
                     NAGHQRYNAFKKFVSEISEISANDINMTIKTGQSRHENVISINMNDAIPRDEKG"
     gene            complement(903..2270)
                     /gene="rhlE"
                     /locus_tag="ACHOUK_002396"
     CDS             complement(903..2270)
                     /gene="rhlE"
                     /locus_tag="ACHOUK_002396"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFP9897838.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRK
                     PAAAQ"
     gene            2499..3170
                     /gene="cecR"
                     /locus_tag="ACHOUK_002397"
     CDS             2499..3170
                     /gene="cecR"
                     /locus_tag="ACHOUK_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFP9897839.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     YRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            3173..4168
                     /gene="hlyD"
                     /locus_tag="ACHOUK_002398"
     CDS             3173..4168
                     /gene="hlyD"
                     /locus_tag="ACHOUK_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFP9897840.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            4161..5897
                     /gene="ybhF"
                     /locus_tag="ACHOUK_002399"
     CDS             4161..5897
                     /gene="ybhF"
                     /locus_tag="ACHOUK_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP9897841.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            5890..7023
                     /gene="ybhS"
                     /locus_tag="ACHOUK_002400"
     CDS             5890..7023
                     /gene="ybhS"
                     /locus_tag="ACHOUK_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9897842.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            7034..8140
                     /gene="ybhR"
                     /locus_tag="ACHOUK_002401"
     CDS             7034..8140
                     /gene="ybhR"
                     /locus_tag="ACHOUK_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9897843.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(8102..8512)
                     /gene="ybhQ"
                     /locus_tag="ACHOUK_002402"
     CDS             complement(8102..8512)
                     /gene="ybhQ"
                     /locus_tag="ACHOUK_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFP9897844.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            8645..9406
                     /gene="ybhP"
                     /locus_tag="ACHOUK_002403"
     CDS             8645..9406
                     /gene="ybhP"
                     /locus_tag="ACHOUK_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFP9897845.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            9403..10644
                     /gene="clsB"
                     /locus_tag="ACHOUK_002404"
     CDS             9403..10644
                     /gene="clsB"
                     /locus_tag="ACHOUK_002404"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFP9897846.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            10644..11600
                     /gene="ybhN"
                     /locus_tag="ACHOUK_002405"
     CDS             10644..11600
                     /gene="ybhN"
                     /locus_tag="ACHOUK_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFP9897847.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHVPAGIGVLEAVFIALLAGEHTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(11636..12349)
                     /gene="ybhM"
                     /locus_tag="ACHOUK_002406"
     CDS             complement(11636..12349)
                     /gene="ybhM"
                     /locus_tag="ACHOUK_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFP9897848.1"
                     /translation="MESYLQNSNKLDFQHETRILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLIKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIVVFSTAGAMFLISMLAGLLFNIDPGSHRFMIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWSGIILHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(12557..13261)
                     /gene="ybhL"
                     /locus_tag="ACHOUK_002407"
     CDS             complement(12557..13261)
                     /gene="ybhL"
                     /locus_tag="ACHOUK_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFP9897849.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDASNLRKYSILCALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(13397..13849)
                     /gene="moaE"
                     /locus_tag="ACHOUK_002408"
     CDS             complement(13397..13849)
                     /gene="moaE"
                     /locus_tag="ACHOUK_002408"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFP9897850.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFIGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(13851..14096)
                     /gene="moaD"
                     /locus_tag="ACHOUK_002409"
     CDS             complement(13851..14096)
                     /gene="moaD"
                     /locus_tag="ACHOUK_002409"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFP9897851.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(14089..14574)
                     /gene="moaC"
                     /locus_tag="ACHOUK_002410"
     CDS             complement(14089..14574)
                     /gene="moaC"
                     /locus_tag="ACHOUK_002410"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFP9897852.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEVD
                     D"
     gene            complement(14577..15089)
                     /gene="moaB"
                     /locus_tag="ACHOUK_002411"
     CDS             complement(14577..15089)
                     /gene="moaB"
                     /locus_tag="ACHOUK_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFP9897853.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(15111..16100)
                     /gene="moaA"
                     /locus_tag="ACHOUK_002412"
     CDS             complement(15111..16100)
                     /gene="moaA"
                     /locus_tag="ACHOUK_002412"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFP9897854.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(16088..16232)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            16497..17405
                     /gene="yvcK"
                     /locus_tag="ACHOUK_002413"
     CDS             16497..17405
                     /gene="yvcK"
                     /locus_tag="ACHOUK_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFP9897855.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDAHLIPMSEYPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHSALEKALQALG"
     gene            complement(17596..19617)
                     /gene="uvrB"
                     /locus_tag="ACHOUK_002414"
     CDS             complement(17596..19617)
                     /gene="uvrB"
                     /locus_tag="ACHOUK_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFP9897856.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(20196..20873)
                     /gene="bioD"
                     /locus_tag="ACHOUK_002415"
     CDS             complement(20196..20873)
                     /gene="bioD"
                     /locus_tag="ACHOUK_002415"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFP9897857.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRSIESSVMSSG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(20866..21621)
                     /gene="bioC"
                     /locus_tag="ACHOUK_002416"
     CDS             complement(20866..21621)
                     /gene="bioC"
                     /locus_tag="ACHOUK_002416"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFP9897858.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRQSADVLLAMLPQRKYP
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHRIQPITLWFDDALSAMRSLKGIGATHLHEGRDQRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIVRE"
     gene            complement(21608..22762)
                     /gene="bioF"
                     /locus_tag="ACHOUK_002417"
     CDS             complement(21608..22762)
                     /gene="bioF"
                     /locus_tag="ACHOUK_002417"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFP9897859.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFVHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     TCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARNLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPSGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(22759..23799)
                     /gene="bioB"
                     /locus_tag="ACHOUK_002418"
     CDS             complement(22759..23799)
                     /gene="bioB"
                     /locus_tag="ACHOUK_002418"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFP9897860.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMSYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            23886..25175
                     /gene="bioA"
                     /locus_tag="ACHOUK_002419"
     CDS             23886..25175
                     /gene="bioA"
                     /locus_tag="ACHOUK_002419"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFP9897861.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQHFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            25234..25710
                     /gene="ybhB"
                     /locus_tag="ACHOUK_002420"
     CDS             25234..25710
                     /gene="ybhB"
                     /locus_tag="ACHOUK_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFP9897862.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            26028..26120
                     /locus_tag="ACHOUK_002421"
                     /pseudo
     CDS             26028..26120
                     /locus_tag="ACHOUK_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(26217..26915)
                     /gene="nleD"
                     /locus_tag="ACHOUK_002422"
     CDS             complement(26217..26915)
                     /gene="nleD"
                     /locus_tag="ACHOUK_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector zinc metalloprotease NleD"
                     /protein_id="HFP9897863.1"
                     /translation="MRPTSLNLVLHQSSRSSSMSDTDIESLVKASSVQWIKNNPQLRF
                     QGTDHNIYQQIEAALDKIGSTETGRVLLNAIESISRLKSETVVIHLNSSRLGVMAHRD
                     IDAENHRGTGSDFHCNLNAVEYPCGEGISVVDFHATIVFHELLHVFHNLNGERLKVES
                     SRAESQKYSPLLLEEARTVGLGAFSEEVLSENKFHEEIGMPRRTSYPHDSALIHDDNT
                     VSLGFQQVRLHPLL"
     gene            complement(26977..27098)
                     /locus_tag="ACHOUK_002423"
                     /pseudo
     CDS             complement(26977..27098)
                     /locus_tag="ACHOUK_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(27146..28027)
                     /gene="nleH1-1"
                     /locus_tag="ACHOUK_002424"
     CDS             complement(27146..28027)
                     /gene="nleH1-1"
                     /locus_tag="ACHOUK_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector kinase
                     NleH1-1"
                     /protein_id="HFP9897864.1"
                     /translation="MLSPYSVNLGCSWNSLTRNLTSPDNRVLSSVRDAAVHSDNGAQV
                     KVGNRTYRVVATDNKFCVTRESHSGCFTNLLHRLGWPKGEISRKIEVMLNASPVSAAM
                     ERGIVHSNRPDLPPVDYAPPELPSVDYNRLSVPGNVIGKGGNAVVYEDAEDATKVLKM
                     FTTSQSNEEVTSEVRCFNQYYGAGSAEKIYGNNGDIIGIRMDKINGESLLNISSLPAQ
                     AEHAIYDMFDRLEQKGILFVDTTETNVLYDRAKNEFNPIDISSYNVSDRSWSESQIMQ
                     SYHGGKQDLISVVLSKI"
     gene            complement(28196..28351)
                     /locus_tag="ACHOUK_002425"
                     /pseudo
     CDS             complement(28196..28351)
                     /locus_tag="ACHOUK_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            28630..28734
                     /locus_tag="ACHOUK_002426"
                     /pseudo
     CDS             28630..28734
                     /locus_tag="ACHOUK_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(28854..29873)
                     /gene="nleC"
                     /locus_tag="ACHOUK_002427"
     CDS             complement(28854..29873)
                     /gene="nleC"
                     /locus_tag="ACHOUK_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector zinc
                     metalloprotease NleC"
                     /protein_id="HFP9897865.1"
                     /translation="MICRVLDINMKIPSLQSNFNFSAPAGYSAPIAPNRAENAYADYV
                     LDIGKRIPLSAADLSNVYESVIRAVHDSRSRLIDQHTVDMIGNTVLDALSRSQTFRDA
                     VSYGIHNEKVHIGCIKYRNEYELNEESSVKIDDIQSLTCNELYEYDVGQEPIFPICEA
                     GENDNEEPYVSFSVAPDTDSYEMPSWQEGLIHEIIHHVTGSSDPSGDSNIELGPTEIL
                     ARRVAQELGWSVPDFKGYAEPEREAHLRLRNLNALRQAAMRHEENERAFFERLGTISD
                     RYEASPDFTEYSAVSNIGYGFIQQHDFPGLAINDNLQDANQIQLYHGAPYIFTFGDVD
                     KHNQQ"
     gene            complement(29907..>30457)
                     /gene="nleB2"
                     /locus_tag="ACHOUK_002428"
     CDS             complement(29907..>30457)
                     /gene="nleB2"
                     /locus_tag="ACHOUK_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB2"
                     /protein_id="HFP9897866.1"
                     /translation="DKRDEYLLNLLEKELREISKAQDSLISMYAKKRNHAWFDFFRNL
                     ALLKAGEIFRCTYNTKNHGISFGEGCIYLDMDMILTGKLGTIYAPDGISMHVDRRNDS
                     VNIENSAIIVNRSNHPALLEGLSFMHSKVDAHPYYDGLGKGVKKYFNFTPLHNYNHFC
                     DFIEFNHPNIIMNTSQYTCSSW"
BASE COUNT         7442 a         7921 c         7508 g         7586 t
ORIGIN      
        1 tccctttctc atcacgagga attgcatcat tcatatttat agagatcaca ttttcatgtc
       61 tggattgccc ggttttaatc gtcatattga tatcatttgc gctgatctcc gaaatctcac
      121 tgacgaattt tttaaatgca ttatagcgct ggtggccagc attaaatttg ccaaatgaaa
      181 cataatatgc gaaatgttca aggaaaccat gtaattccag taaattaacc gggtcagaac
      241 cctgcaccat atttaataac atcgtcgcat aaccattgca ttttgaacaa tccatacatc
      301 gctcctttgc acttgaatga tctgcgaatc ataagcctgg caaaatgggg agtcttacag
      361 aaatcgctca tttttaactt ttcgtactgc aaattcagga tcaacctggt tatgaaagtt
      421 tgctgctgta cggctctaaa cagactggca atcaatcttt ctatcaataa gctgaaatga
      481 gcgatttttg atagtttatt agaactacta aagatatacg gtaatgaaac gactgcacac
      541 tttattttgc ttagggagat agtcggatac actatagaca atgaaaataa taaaatcagg
      601 gacagaatca agctaatact gaatcacata tctcataata atttaccaga tgatgaacaa
      661 actctttatg caatagcatg tttattccag attttcccag aatttgttta tgaatatcaa
      721 ccaatgttat taacaataat tcaaattgca aggagaaata atttcattgc cattttagat
      781 acactagata gatcataaag caaatttatt aaaacaataa aatcagacat gtcatgcatg
      841 tctgaccgtg ttacctgaat aatgggcaaa agcctgtgat actcttttgc ccttacatgg
      901 tattactgcg cagcggcagg tttacgcgga cggcgacggc gttgttgttc gcctgccggt
      961 ttggcatcgc caagacgacg agatggtttt tctgcaggct tcgcgctagc tgatttcgca
     1021 ccaccttctc cacggcgtgg ttgctgttga ccgcgaccac caccaccttg cccacgaccg
     1081 ccgccgccac gttgctggcg accgttctgg atcggttcgg ctttgattga cgggtccggc
     1141 tcataacccg gaatcgcaat gcgcgggatc tcttttttca gcagtttttc gatatcacgc
     1201 agcagtttgt gttcatcaac acacaccagc gacaatgctt cgccagttgc cgccgcacgg
     1261 ccagtacgcc cgatacggtg gacataatct tccggtacgt ttggcagttc atagttgacg
     1321 acgtgcggca actcttcgat atccagaccg cgcgcagcga tgtcagttgc caccagtaca
     1381 cgaatatcgc ccgatttaaa atcggccagc gcacgggtac gcgcaccttg cgatttatta
     1441 ccgtggatcg ccgcactacg gatgccatct ttattaagct gttcagcgag atggttagcg
     1501 ccatgtttgg tacgggtaaa caccagcacc tgctgccagt tccctttccc aatcatgtgc
     1561 gacagcaatt cgcgcttgcg tttcttatca acaaagtgaa catgctgggt cacctgatca
     1621 gacgcggtat tgcggcgtgc cacttctatt tccagcgggt tgtgcaacag tttttccgcc
     1681 agggctttaa tatcgtcaga gaaggtcgcg gagaacaaca ggttctggcg cttcgcaggt
     1741 agttttgtta acacgcgacg gatatcgtgg ataaagccca tgtcgagcat gcggtcagct
     1801 tcatcgagga cgaggatttc aacctgatcc agcttcactg cattctgatg ttccaggtcc
     1861 agcaaacgtc ccggcgttgc caccagcaca tcaacgccgc cgcgcaactt catcatttgt
     1921 gggttaatgc tgacaccgcc aaacaccacc agcgaacgaa tgttcaggta tttgctgtaa
     1981 tcacggacgt tttcgccaat ctgcgccgcc agttcacggg tcggggtaag aatgagcgca
     2041 cgtaccggac gacgcccttt ggcgtgcggc tggcgagtga tcaggtgttg caacagcggc
     2101 agcgtaaagc ccgctgtttt gccggtgccg gtctgggcgc tagccatcag gtcgcggcct
     2161 tccagcaccg cagggatcgc ctgctgctga ataggggtgg gttcacggta accctgctcg
     2221 gcaacggcgc gcaggatatc agggcttaaa cccaaagaat cgaaagacat aactactccg
     2281 aaccgccccg accgttaccg gtgtagtttt cagggagata ctgaaaagga aaatgacaaa
     2341 aaccacaatg tcatgaggga gcggagtgta gcagtttttg tgacgcagcg cataaattat
     2401 ccctgtgact ggcgtagcta aaattcttag ccatactgga caactcccat atggagtcat
     2461 aatcttaatc aatcatttga ttaagaatgg actgtgcgat gaataatcct gccatgacaa
     2521 tcaagggtga acaggcgaaa aaacagctga ttgctgccgc actggcgcag tttggtgaat
     2581 atggaatgaa cgccaccacc cgcgagatag ccgcccaggc cgggcagaat atcgctgcca
     2641 tcacctacta cttcggttcg aaagaagatt tgtacctcgc ctgcgcccag tggattgccg
     2701 attttattgg cgagcagttc cgtccgcatg ccgaggaagc cgaacgcttg ttcgcccaac
     2761 cacagcctga tcgggctgcc atccgtgaac tgatccttcg cgcctacagg aacatgatta
     2821 agctgctcac ccaggatgac accgtcaacc tcagcaagtt tatctcccgt gagcagctct
     2881 ctcccacggc agcctaccac ctggtgcacg aacaggtgat tagtccgcta cacagccacc
     2941 tgacacgcct gattgccgcc tggaccggct gcgacgccaa tgacacccgc atgatccttc
     3001 atacccatgc gttgattggt gagattctgg cgttccgtct tggcaaagaa acaattctgt
     3061 tacgtaccgg ctggaccgcg ttcgatgaag aaaagaccga actgatcaac cagacggtga
     3121 cctgtcatat cgacctgatt ttgcaaggat tatcgcaaag gagtttgtag tgatgaaaaa
     3181 acctgtcgtg atcggactgg cggtagtggt acttgccgcc gtggttgccg gaggctactg
     3241 gtggtatcaa agccgccagg ataacggcct gacgctgtat ggcaacgtgg atattcgtac
     3301 ggtgaatctt agtttccgtg ttggggggcg cgttgaatcg ctggcggtgg acgaaggtga
     3361 tgctatcaaa gcgggccagg tgctgggcga actggatcac aagccgtatg agattgccct
     3421 gatgcaggcg aaagcgggcg tctcggtggc acaggcgcag tatgacctga tgcttgccgg
     3481 gtatcgcgat gaagaaatcg ctcaggccgc cgcagcggtg aaacaggcgc aagccgccta
     3541 tgactatgcg cagaacttct ataaccgcca gcaaggactg tggaaaagcc gcaccatttc
     3601 ggcaaatgac ctggaaaatg cccgctcctc gcgcgaccag gcacaggcaa cgctgaaatc
     3661 agcacaggat aaattgcgtc agtaccgttc cggtaaccgt gaacaggaca tcgctcaggc
     3721 gaaagccagc ctcgaacagg cgcaggcgca actggcgcag gcggagctga atttacagga
     3781 ctcaacgttg atagcaccgt ctgatggcac gctgttaacg cgcgcggtgg agccaggcac
     3841 ggttctcaat gaaggtggca cggtgtttac cgtttcacta acgcgtccgg tgtgggtgcg
     3901 tgcttatgtt gatgaacgga atcttgacca ggcccagccg gggcgcaaag tgctgcttta
     3961 taccgatggt cgcccggaca agccctatca cgggcagatt ggtttcgttt cgccgactgc
     4021 tgaatttacc ccgaaaaccg tcgaaacacc ggatctgcgt accgacctcg tctatcgcct
     4081 gcgtattgtg gtgaccgacg ccgatgatgc gttacgccag ggaatgccag tgacggtaca
     4141 attcggtgac gaggcaggac atgaatgatg ccgttatcac gctgaacggc ctggaaaaac
     4201 gctttccggg catggacaag cccgccgtcg cgccgctcga ttgcaccatt catgccggtt
     4261 atgtgacggg gctggtaggg ccagacggtg cgggtaaaac cacgctgatg cggatgctgg
     4321 cgggattact gaaacccgac agcggcaatg ccacggtgat tggctttgat ccgatcaaaa
     4381 acgacggcgc gctgcacgcc gtgctcggct atatgccgca gaaattcggc ctgtatgaag
     4441 atctgacggt gatggagaac ctcaatctgt acgcggattt gcgcagcgtc accggcgagg
     4501 cacggaagca gacttttgct cgcctgctgg agtttacgtc tcttggcccg tttaccggac
     4561 gcctggcggg caagctctcc ggtgggatga aacaaaaact cggcctggcc tgtaccctgg
     4621 tgggcgaacc gaaagtgttg ctgctcgatg aacccggcgt cggcgttgac cctatctcac
     4681 ggcgcgaact gtggcagatg gtgcatgagc tggcgggcga agggatgtta atcctctgga
     4741 gtacctcgta tctcgacgaa gccgagcagt gccgtgacgt gttactgatg aacgaaggcg
     4801 agttgctgta tcagggagaa ccaaaagccc tgacacaaac catggccgga cgcagctttc
     4861 tgatgaccag tccacacgag ggcaaccgca aactgttgca acgcgccttg aaactgccgc
     4921 aggtcagcga cggcatgatt caggggaaat cggtacgtct gatcctcaaa aaagaggcca
     4981 caccagacga tattcgccat gccgacggga tgccggaaat caacatcaac gaaactacgc
     5041 cgcgttttga agatgcgttt attgatttgc tgggcggtgc cggaacctca gaatcgccgc
     5101 tgggtgcaat attgcatacg gtagaaggca cacccggcga gacggtgatc gaagcgaaag
     5161 aactgaccaa gaaatttggt gattttgccg ccaccgatca cgtcaacttt gccgttaaac
     5221 gcggggagat ttttggtttg ctggggccaa acggcgcggg caaatcgacc acctttaaga
     5281 tgatgtgcgg tttgctggtg ccgacttccg gccaggcgct ggtgctgggg atggatctga
     5341 aagagagttc cggtaaagcg cgccagcatc tcggctatat ggcgcaaaaa ttttcgctct
     5401 acggcaacct gacggtcgaa cagaatttac gctttttctc tggtgtgtat ggcttacgcg
     5461 gtcgggcgca gaacgaaaaa atctcccgta tgagcgaggc atttggcctg aaaagtattg
     5521 cctcacacgc caccgatgaa ctgccattag gttttaaaca gcggctggcg ctggcctgtt
     5581 cgctgatgca tgaaccggat attctgtttc tcgacgaacc gacttccggc gttgaccccc
     5641 tcacccgccg tgaattctgg ctgcacatca acagcatggt agagaaaggc gtcacggtga
     5701 tggtcaccac ccactttatg gatgaagcgg aatattgcga ccgcatcggc ctggtgtacc
     5761 gcgggaaatt aatcgccagc ggcacgccgg acgatttgaa agcacagtcg gctaacgatg
     5821 agcaacccga tcccaccatg gagcaagcct ttattcagtt gatccacgac tgggataagg
     5881 agcatagcaa tgagtaaccc gatcctttcc tggcgtcgcg tgcgggcgct gtgcgtcaaa
     5941 gagacgcggc agatcgttcg cgatccgagt agctggctga ttgcggtagt gatcccgctg
     6001 ttgctgctgt ttatttttgg ttatggcatt aacctcgact ccagcaagct gcgggtcggg
     6061 attttactgg aacagcgtag cgaagcagcg ctggatttca cccacaccat gaccggttcg
     6121 ccctacatcg acgccaccat cagcgataac cgtcaggaac tgatcgccaa aatgcaggcg
     6181 gggaaaattc gcggtctggt ggttattccc gtggatttcg ccgaacagat ggagcgcgcc
     6241 aacgctaccg caccgattca ggtgatcacc gacggcagtg agccgaatac cgctaacttt
     6301 gtgcagggat atgtcgaagg gatctggcag atctggcaaa tgcagcgggc ggaggataac
     6361 gggcagacct ttgagccgct tattgatgta caaacccgct actggtttaa cccggcggcg
     6421 attagccagc actttattat ccccggtgcg gtgaccatca tcatgacggt catcggcgcg
     6481 attctcacct cgctggtggt ggcgcgagaa tgggaacgcg gcactatgga ggctctgctc
     6541 tctacggaga ttacccgcac ggaactgctg ctgtgtaagc tgatccctta ttactttctc
     6601 gggatgctgg cgatgttgct gtgtatgctg gtgtcggtgt ttattctcgg cgtgccgtat
     6661 cgcggttcgc tgctgattct gttttttatc tccagcctgt ttttactcag taccctgggg
     6721 atggggctgc tgatttccac gattacccgc aaccagttca atgccgctca ggtcgccctg
     6781 aacgccgcgt tcctgccgtc gattatgctt tccggcttta tttttcagat cgacagtatg
     6841 cccgcggtga tccgcgcggt gacgtacatt attcccgcac gttatttcgt cagcaccctg
     6901 caaagcctgt tcctcgccgg gaatattcca gtggtgctgg tggtgaacgt actgtttttg
     6961 atcgcttcgg cggtgatgtt tatcggcctg acgtggctga aaaccaaacg tcggctggat
     7021 tagggagaag agcatgtttc atcgcttatg gacgttaatc cgcaaagagt tgcagtcgtt
     7081 gctgcgcgaa ccacaaaccc gcgcgattct gattttaccg gtgctgattc aggtgattct
     7141 gttcccgttc gccgccacgc tggaagtgac caacgccacc atcgccatct acgatgaaga
     7201 taacggcgag cattcggtgg agctgaccca acgttttgcc cgcgccagcg cctttacaca
     7261 tgtgctgctg ctgaaaagcc cacaggagat ccgcccgacc atcgacacac aaaaggcgtt
     7321 actactggtg cgtttcccgg ctgacttctc gcgcaaactg gataccttcc agaccgcgcc
     7381 gttgcagttg atcctcgacg ggcgtaactc caacagtgcg caaattgccg ccaactacct
     7441 gcaacagatc gtcaaaaatt atcagcagga gctgctggaa ggaaaaccga aacctaacaa
     7501 cagcgagctg gtggtacgca actggtataa cccgaatctc gactacaaat ggtttgtggt
     7561 gccgtcgctg atcgccatga tcaccactat tggcgtaatg atcgtcactt cactttccgt
     7621 cgcccgcgaa cgtgaacaag gtacgctcga tcagctactg gtttcgccgc tcaccacctg
     7681 gcagatcttc atcggcaaag ccgtaccggc gttaattgtc gccaccttcc aggccaccat
     7741 tgtgctggcg attggtatct gggcgtatca aatccccttc gccggatcgc tggcgctgtt
     7801 ctactttacg atggtgattt atggtttatc gctggtggga ttcggtctgt tgatttcatc
     7861 actctgttca acacaacagc aggcgtttat cggcgtgttt gtctttatga tgcccgccat
     7921 tctcctttcc ggttacgttt cgccggtgga aaacatgcca gtatggctgc aaaacctgac
     7981 gtggattaat cctattcgcc actttacgga cattaccaag cagatttatt tgaaggatgc
     8041 gagtctggat attgtgtgga atagtttgtg gccgctactg gtgataacgg ccacgacagg
     8101 gtcagcggcg tacgcgatgt ttagacgtaa ggtgatgtaa cttcttatct ttcgccagca
     8161 aagacactac cgccgggccg gcaaggattg ccagccctgc aattgccagc aaaaagttgt
     8221 tttccagtac gcgggacaac agccacagca caatcagcgc tgcgccaaaa taccaggtcg
     8281 tggtcagctc ttccagcacg tctgccacct cacgccagcg ttgctcgggg tggcgctgaa
     8341 acaccagcat catcactacc gtgacacaat acagtgcgca taatcccacg ccgacgtgaa
     8401 ccagagtctt actcatccag cccaccacca gcagcgccac taccagtaaa tgcaacataa
     8461 tctgccagca acttagaccc gttgcgacac gaacacgttg ttgccacttc atggcttctc
     8521 tcctgaagaa tttatctcta cttactcaga gtaccgaact ttacggaaaa ttccgtaaga
     8581 ccgcaccttt ttgtgacgcc gactacacta tttcaatgag ataagaggca aagacaggaa
     8641 aattatgccc gatcaaacac aacaattttc gttcaaggtg ctcaccatca atattcacaa
     8701 aggctttacc gcgtttaacc gacgcttcat tttgccggaa cttcgcgatg ccgtgcgtac
     8761 cgtcagcgcc gatattgttt gcctgcagga agtgatgggc gcgcacgaag ttcatccgct
     8821 gcatgtggaa aactggcccg atacctcgca ctacgagttt ctcgccgaca ctatgtggag
     8881 cgattttgcc tacggtcgca atgccgtata cccggaaggg catcacggca acgccgtact
     8941 gtcgcgttat cccattgaac attatgagaa tcgcgatgtt tcggtcgatg gtgcggaaaa
     9001 gcgcggcgtg ctctactgcc gcattgtgcc gccgatgacc ggaaaagcga ttcatgtgat
     9061 gtgcgtacat ctgggcctgc gtgaggcgca ccgtcaggcg cagcttgcga tgctcgccga
     9121 atgggtgaat gagctaccgg acggcgaacc ggtattggtg gcgggtgatt tcaatgactg
     9181 gcggcaaaaa gctaatcatc cgttaaaagt gcaggccgga ctggatgaga tttttacccg
     9241 cgcccacgga cgcccggcgc gcacgtttcc ggtgcaattt cctctactac gactggacag
     9301 gatctacgtc aaaaatgcca gcgccagcgc gccaaccgcg ttgccgctgc ggacatggcg
     9361 acacctttct gatcatgccc ctttaagtgc ggagattcat ttatgaaatg tagctggcgc
     9421 gaaggcaata agatccagtt gctggaaaac ggcgagcaat attatcccgc ggtgtttaag
     9481 gcgattggcg aggcacaaga acgcatcatt cttgaaacgt ttatctggtt tgaggatgac
     9541 gtcggcaaac aactgcatgc ggcactactg gcagcagcgc aacgcggggt taaagcggaa
     9601 gtcttgctgg atggctacgg ttcgccggat ctcagcgatg agtttgtcaa tgaactgacg
     9661 gcagctggcg tagtgttccg ctactacgat ccccgccctc gcctttttgg tatgcgcacc
     9721 aatgtgtttc gccggatgca tcgcaaaatt gtggtaatcg acgcgcgtat cgcctttatt
     9781 ggcgggctga attactccgc cgagcatatg tccagctacg gtccagaggc taaacaggat
     9841 tacgcggtac gccttgaagg gccgattgtc gaagatattc tccagtttga gctggaaaac
     9901 ctgcctggac agagcgcggc acgacgctgg tggcgacgtc atcacaaagc ggaagagaac
     9961 cgccagccgg gagaagcgca ggtattgctg gtctggcgcg ataacgaaga acatcgcgat
    10021 gatattgaac gtcactatct gaaaatgctc actcaggcgc ggcgagaagt cattatcgcc
    10081 aacgcctact tcttccccgg ctatcgattt ttacacgcct tgcgtaaagc ggcacggcgc
    10141 ggggtgcgga tcaaactgat cattcagggc gaaccggata tgccgattgt cagagtcggt
    10201 gcgcgcttgc tgtataacta tctggttaaa ggcggcgttc aggtgtttga gtaccgccgc
    10261 cgcccgctcc acggcaaagt ggcattgatg gacgatcact gggcgacagt agggtccagt
    10321 aatctcgatc cgctcagttt gtcactgaat ctcgaagcaa atgtcatcat ccacgatcgt
    10381 cattttaacc agacgctgcg cgataatctg aacggcatta ttgccgcaga ttgtcagcag
    10441 gtcgatgaga ccatgctgcc gaaacgcacc tggtggaacc taaccaaaag cgtgctggcg
    10501 ttccactttt tacgccactt cccggcgctg gttggctggc ttccggcaca cacgccacgt
    10561 ctggcgcagg ttgatcctcc cgcacaaccg acaatggaaa cgcaggatcg ggtagaaact
    10621 gaaaacacgg gggtaaaacc ctgatgagta aatcacaccc gcgctggcgc ttagcaaaga
    10681 agatcctcac ctggctgttt tttatcgcgg tgattgtgtt gctggtggtc tacgccaaaa
    10741 aagtggactg ggaagaggtc tggaaggtca tccgcgacta caatcgcgtt gcgctgctta
    10801 gtgcggtcgg gctggtggtc gtcagctatc tgatttacgg ctgctatgac ctgctcgccc
    10861 gcttttactg cggtcacaaa ctggcgaagc gccaggtgat gctggtgtcg tttatctgct
    10921 acgccttcaa cctgacgctc agtacctggg tcggcggcat tggtatgcgc tatcgtttgt
    10981 actcccgtct gggattgccg ggcagcacta ttacgcggat tttctcgctc agtattacca
    11041 ccaactggct gggctatatt ttactggcag ggattatctt taccgcaggc gtggtggagt
    11101 tgccggacca ctggtatgtc gatcaaacta cgctgcgtat tctcggcatt ggcttactga
    11161 tgattatcgc ggtttatttg tggttttgcg ctttcgcgaa gcaccgccat atgaccatca
    11221 aagggcaaaa gctggtgctg ccttcgtgga aattcgccct cgcccagatg ctgatttcca
    11281 gcgttaactg gatggtcatg ggggcgatta tctggctgtt acttggtcaa agcgtgaact
    11341 atttctttgt actgggcgtg ttgctggtta gcagtattgc cggcgtcatc gtgcatgttc
    11401 cggcggggat tggtgtgctg gaagcggtgt ttatcgcgct actggctggg gagcatacat
    11461 ccaaaggcac aattatcgcc gccctactcg cttaccgtgt gctgtattac tttatcccgc
    11521 tgctgctggc gctgatctgt tacctgttgc tggaaagcca ggcgaagaag ctacgggcga
    11581 aaaatgaagc ggcgatgtga agtttagtta agttctttag tatgtgcatt tacgactaat
    11641 gaaaaaaacg cgtatgcctt tgccagacaa gcgttatagc tatcgcggca agtatcccaa
    11701 agaagccgat aaaatagtaa tagagtgtta atgcacctgt aaaagcgcaa cgaaccggac
    11761 tccacaactc ttcactatgg catttccccg ccaattcaag gagcttattt cgtccatgca
    11821 agataatgcc tgaccataac acaatcatta agcaacttat tacccaaatg ggccgttcac
    11881 tcattaatgc ggcattcacg atgattacca gggccaaccc tgtcaacgtc atcataatca
    11941 taaaacggtg agaaccagga tcaatattaa ataataaacc ggccagcata ctgattaaaa
    12001 acatcgcgcc cgcagtgctg aacacgacga taatggcata tacaggaact aaacttgcaa
    12061 cgacaatcgc caccaaagag agtaaaaaca gatatattac gcctgcgatg gcggcattta
    12121 tcttgttgat atccttaatt aagccataca tacaaagtaa taataaccct atggcgacat
    12181 acattggtgg tgaatcatat ttggttgccg taatttcgag atacttagcc ccccaggcca
    12241 gccctgcggt tgccaccaaa cctaaagcgg taatgagcca tataccgttt aatatcctgg
    12301 tctcgtgctg aaaatctaat ttattgctgt tttgcaagta tgattccatt cttttagttg
    12361 tccttataga aaaatattaa tattcatcat gaataaattt aaacccacgc atacaaaata
    12421 cttttgtatt tatggatacg gaaaactctt ccgctcggcc ataaggcgta ttttatacct
    12481 ggtacttgtt aagtgtcgtt tttagtaaga aaaaaaatcc cgacacatgg ccgggatatt
    12541 gatggtgaaa aaagaattaa cgacgattgc caaaaattcg cagcaacatc aggaacaggt
    12601 tgatgaagtc cagatacaag gttaatgcgc aaagaatgga atatttgcgc aggttcgacg
    12661 cgtcgcgggt atctatctgc tcacccatat ttttcagttt ctgggtgtca tacgccgtca
    12721 atccgacaaa gacaatcacg ccgatgtagg tgactgccca catcaacgct tcgcttttca
    12781 gccagaagtt gaccagcgat gccaacacaa tgccgattaa cgccataaac agcatattgc
    12841 caaagccact taaatcgcgc ttcgtggtgt aaccgtacag gctcattgcg ccgaacatcc
    12901 cggcagtaac gacgaaggta ctggcgatag aagcagcggt ataaacaatg aaaatactgg
    12961 aaagcgtaag acccgtcagc gccgaataaa gcataaagag catcgtcgtt acacctgcgc
    13021 tcagcttttg aatcatcgct gataacacaa taaccaatgc taattgcgcg atgatcagac
    13081 cgattaaaaa gacacggtta gtgaacaaca gctccatcac ggccgcggaa ttagccgcat
    13141 accaggcaac aaatgcggtc agcaataagc caacggtcat ccagccatag acttgcgcca
    13201 tataagtctg caagccagcc cggggttgta caattgaatc agaacgtggg aatctgtcca
    13261 tgacgatctc ctgaagatat aaggaatatc ttaaggatac tgcaaaatga tgaggctgtg
    13321 catcgacgca gcgtaaacgc atgtactgag cggtgaaatt gccggacgca gcggtgcctt
    13381 atccggctaa caaaaactac cagcgttttg ccgcctgctg atcgctctcc cgagcttcga
    13441 cccagcggtc gccctccggc gtggcttcgc gcttccagaa cggtgcgcgg gttttgagat
    13501 aatccataat aaactgcccg gcttcaaacg cactgctgcg atgcgcactg gtgacaccga
    13561 taaaaacgat ttcatcgccc ggccataatt ccccgatgcg gtgaatcacc gtgacgcgcc
    13621 ccagcggcca gcggttacgc gcttcatcga caatttctgc cagtgctttt tcagtcatcc
    13681 ccggatagtg ttcgagggtt aatgctttga cgctgtcgcc caggttatgg ttgcgcacct
    13741 taccggtaaa ggtgactacc gcaccgtctt cgtcacgctc cgccagccac gggtactctt
    13801 ctcctacgct gaacggctgc ggaccaacaa caattttggt ttctgccatc ttaacctccg
    13861 gttaccggcg ggaagaaagc tacttcgtcg ccgtcagtca gcggatggtc aaaactcacc
    13921 agcgtctggt tgacggcagc cagtaatttg ccatcttcca gcgccagcgc ccagcgatcg
    13981 ctctgcgcag ccaggtgctg gcgtaacgct tcaacggttg ggaaatccgc agccacttcg
    14041 gttgcatctg ttcctaccaa ctcgcgcacc tgggcgaaaa aaagaacttt aatcatccac
    14101 ttccacctta aagtcacccg acttgccgcc gctcttcgcc agcaaacgta ccggaccaat
    14161 caccatatct ttttgcaccg ctttgcacat gtcataaatg gtcagcgccg ccacggaggc
    14221 cgcggttaac gcttccattt cgacaccggt tttcccggtc aggcggcata aggtttctat
    14281 acgcacccga ttgtgctccg gctcggcctg taaattgact tcaactttgc tgagcatcag
    14341 cggatgacac agcgggatca gatcccaggt gcgttttgcc gcctgaatac cggcaatacg
    14401 cgcagtggca aatacgtcgc ctttgtggtg gcgaccatca ataatcatcg ccagcgtctc
    14461 gctgcgcatg gtgacaaagg cttccgcccg cgcttcacgc acggtttccg ctttggcgga
    14521 gacatccacc atgtgcgctt cgccagcggc gttgatatgg gtcagttgcg acatacttat
    14581 ttcttcaaat gtggatggaa attacacgga cgcgtacggg catccagctg cggcgcgatg
    14641 atattttccc atgcggtacg gcacgctttg gtcgaacccg gcatggcgaa aatcagcgtt
    14701 ttgttagcga cgcccgctac cgcacgagat tgcaacgtgg aagtgccaat ctcttcaaac
    14761 gacaacatac ggaacacttc accaaaacct tcaacttcac ggtcgaacaa cggcagcaat
    14821 gcttcgggag cctgatcacc ttccgtcagg ccagtccccc ccgtaatcaa caccacttgt
    14881 acatcgtcgc tggcgatcca cgcagatacc tgagcgcgaa tagcgtagcg gttttctttc
    14941 acaatggctt tatcgacaac gtgatggccc gcttcttgcg ccgaatcgcg caaatagtga
    15001 ccggaggtat cgtcttcttc accgcgacga ttagaaaccg taagaatagc aatacgggtc
    15061 gggataaatt cagtgcttac ctgactcatc tgatctctcc ttttgacgtt ttagccgcca
    15121 atgtacgata agttttgcgt aataccggtg ttgttttgat gcaggaaatg ggtctgtttc
    15181 ttctcccgca gcgccgctga aatacgcgct tccagcgcct gttgctgggt atcgtcttcc
    15241 agcagatcgc gcaggttaac gccgccttca ccaaacaggc agagatggag tttaccaatg
    15301 gaggaaacgc gcagacggtt gcaagtggcg cagaagtctt tttcatacgg catgataagg
    15361 ccaatctctc cggcgtaatc cggatgacaa aagacttgcg cgggaccgtc gctgcgttga
    15421 cgtaattggt ggatccagcc gcgacgcagt agctcgtcac gcagaacctg accagagatg
    15481 tgatgcttac ggaaaagctc gctgccctcg cccgtttcca tcagttcgat gaaacgcagc
    15541 tggatagggc gatgctggat ccagttcaga aaggtgtcga gctggtgatg attcacatca
    15601 cgcatcagca cggtattgac tttgaccttc tcaaaaccgg cctcaaatgc agcatcgatc
    15661 cctgccatca cctgattgaa tttatcctgg ccggtaatgg catgaaactg gcgggcgtcc
    15721 agactgtcga cgctgacgtt aatgccagta agtcccgcat cgcgccagtt cgccacatcg
    15781 cgttccagac ggtaaccatt ggtggtgacc gctatctggc gaatagcgtc gttttctcgc
    15841 acagcggcga tgatatcggt aaagtcgcgg cgtaaagacg gctcaccgcc tgtcaggcgc
    15901 actttttcgg tgcccagact ggcgaaggcg cgcgtaaccc ggcgaatttc atcgacggtc
    15961 agaaagcctt tattggtgac gccgctcggt ttgtagccat ccggcaggca gtaggtgcaa
    16021 cgaaagttac acacatcggt aatcgacagg cgcaagtagt aaaacttacg cgcaaatgca
    16081 tcagtcagtt gtgaagccat gtacaccttt ccagatacgg gaggcgaagt catttcttcc
    16141 ttcgccctgg tggcaatctt tacgagcatt gtcacggcca aagcaccata tcagttgacc
    16201 caggtgcaga ggctagagtg tttagtggtt aagccgatac tagcgtgtaa atgagatttt
    16261 taccatccac actttcgcta tgtaatcatg tatatagcgt cctgatcgtg ctatttcgct
    16321 acagaataag ggaaaatggc ggttttactt tgatatacat catcagggag gcatagcgtg
    16381 catcgtcctt tagaggtaga agaaaatagt ttgtatcctc aatattggca ggttaattgc
    16441 tgtttccccg caatttgcgc tactgtagcg cggataatct gactccagga aactatatgc
    16501 gcaatcgtac gctggctgat cttgatcgtg tcgttgctct cggcggaggg catggactgg
    16561 gacgcgttct ctcatcactt tcgtctttgg gttctcgttt aacgggtatc gtcaccacca
    16621 cggataatgg tggctcgacg gggcgtattc gccgttcaga aggcggcatt gcctggggcg
    16681 atatgcgcaa ctgccttaac cagctgataa ctgaacccag cgtcgcttca gcaatgtttg
    16741 aatatcgttt cggcggtaat ggcgaacttt ccgggcacaa ccttggaaac ctgatgttga
    16801 aggcactgga tcaccttagc gtgcgacctc ttgaagcgat caatctgatt cgaaatctgc
    16861 tgaaagtgga cgcacatttg attccgatgt cggagtatcc tgttgatctg atggcgattg
    16921 acgatcaggg gcatgaagtt tacggcgagg tcaatatcga ccagttaact acgccgattc
    16981 aagagttatt gttaacgcct aatgtacccg caacgcgtga ggcggttcac gctatcaatg
    17041 aagcggatct catcattatt gggcctggca gtttttacac cagcctgatg ccaattctgc
    17101 tgctgaagga aatcgcgcag gcattacgcc gcacgccagc gccgatggtc tatatcggca
    17161 atctggggcg tgagttgagt ttacctgcgg ccaatttgaa gctggaaagc aagctggcaa
    17221 ttatggagca gtatgttggt aaaaaggtca ttgatgcggt catcgtcggg ccaaaagtgg
    17281 atgtctcggc ggtgaaagag cggattgtga ttcaggaggt gctggaggcc agcgatattc
    17341 cgtatcgtca tgaccgccag ttgttacata gcgcactgga aaaggcgtta caggctttag
    17401 gttaacggaa atccgaagga aaattccggc ttcctaatga agacaaagtg cgcgttgttt
    17461 atgacggatg cggcgtgaac gccttatccg gcctacaaac cgcgctaatt caatgtattg
    17521 cagaatcatg taggcctgat aagcgtagca catcaggcaa ttttgctttt gtcatctgtc
    17581 ttcttcgtta tcctgtcacg aagccgcgat aaacaattca cgcagctgat gcaactggtc
    17641 acgaatttgt gccgcttctt cgaactccag attttgcgcg tgttgcatca ttaacccttc
    17701 cagctcatgg attttctgtt gcaacgcttt aggcgacata tccatcggca cattatccgg
    17761 ctcaacaatc ggtcgcgatt ttcctctgcc cttcgctttg gttttagcaa tgttctgccc
    17821 caacgccagg atatcgacca ctttcttgtt caagccttgc ggcgtaatgc cgtgttcctc
    17881 gttgtacttc tgctgtttct cgcggcgacg ttcggtttcg ccaatcgctt tcgccattga
    17941 cggggtgatc ttatcgccgt agagaatcgc tttaccgtta acgttacgcg ccgcacgacc
    18001 aatagtctgg atcaacgaac gttcagaacg caggaagcct tctttgtcgg cgtcgaggat
    18061 cgccaccagc gaaacctccg gcatatccag accttcgcgc agtaagttga tccccaccag
    18121 cacgtcgaac tcgcccagac gcaagtcgcg gataatctcc atacgttcga cggtgtcgat
    18181 atctgagtga agataacgca cgcgctcacc gtgttcttcg agatattcgg taagatcttc
    18241 cgccattcgc ttggtcagtg tggtgaccag tacgcgttcg ttaattgccg ctcgctgacg
    18301 aatctccgaa agaagatcat caacctgtgt cgccactggc cgcacttcga taatcggatc
    18361 aagcaatccg gttggacgca ccacctgatc caccacatcg ccgccggatt tttccagctc
    18421 ataattacct ggcgtcgccg aaacatagat ggtttgcggc gctaatgctt cgaactcttc
    18481 aaatttcagc ggacggttat ccagcgctga tggcaggcgg aagccgtact ccaccagtgt
    18541 ctctttacgc gcccggtcac cgcgatacat gccgccaatt tgtggaatgg tgacgtgaga
    18601 ttcatcgacc accagcagcc catcggcagg caggtaatca aacagcgtcg gcggtggctc
    18661 acccggtcca cgaccggaga ggaagcgcga gtagttttca atccccgaac agtagcccag
    18721 ctcgttcatc atctccagat caaactgggt acgctgggtc agccgctgct cttccagcag
    18781 tttgttgttt tccaatagca ctttgcgtct ggcggccagc tcttctttga tctcctccat
    18841 cgcctgtacg atgcgctcgc gcggtgtgac gtagtgcgtt ttcgggtaga tggtaaaacg
    18901 tggaatggtg gaaacaatct gcccggttag cgggtcaaat aacgacaatc gttccacttc
    18961 ctcgtcaaac agttccacgc gaagtgcaat gtcatccgat tctgccggga agatatcaat
    19021 cacctcgcca cgaacacgga aagtaccacg ctggaatgct tgatcattac gagcgtattg
    19081 cagctccgcc agtcggcgca gaatcgcgcg ctgatcgata atcataccga ccgtgagatg
    19141 gagcatcatc ttgagatata aatcaggatc gcccagacca taaatcgcgg aaacagacgc
    19201 gaccacaaca acatcacgcc gctccagcat cgctttggtg gcggacaaac gcatctgttc
    19261 gatatgttcg tttaccgagg catctttctc aatgaaggtg tcggaactcg gtacataggc
    19321 ttctggctga tagtagtcgt agtaggagac gaaatattcc accgcgtttt ccgggaagaa
    19381 ctctttcatt tcgccataca gctgggccgc cagcgttttg ttgggcgcaa gtaccatggt
    19441 agggcgctga aggtcagcaa tgacattggc aatggtgaag gttttcccgg aaccagtcac
    19501 accaagtaac gtctggtgcg ccaggccatc ttccagcccc tcttcgagac gtcgaatcgc
    19561 ctctggctga tcgccagaag gtttaaaagc ggaattcagt ttgaacggtt tactcatgag
    19621 tcgctacctg aaggagttgg gcgggcaggt atgtaatttt actcgtcgta cttaattatg
    19681 ccaacaaatt atactggata aaaaaacagt tcatcaccat aatatttctg ttacagcgta
    19741 aactccgctg tcaatcatga gtaaaattta ctctgtggcg agataaaact cggaccttac
    19801 cgggttatcc ccaaagcaac ggctttttta acatttgtca agatgagtga tgtcagtttt
    19861 atggcaagag atgcctgttc agtgactgcc attgcaatta tctaaccagt taaaaaataa
    19921 aagatatttc tttgagccgt ctttaacgcc aattcgcgtg caagccgcgt attctctcgc
    19981 ttgcctcgtg ttttctaact ctaatacaca tggttatcca caggaatagt ggataactgt
    20041 ctgcagcccc tatcccccgc cgcctgggca attcccacat tcgccgcatg atgcggaagt
    20101 aaaatttttt tcgtgattat tttagaattt aattggttaa attgcagtca atcgaagacg
    20161 cgatctcgct cgcaatttaa ccaaatacag gatggctaca acaaggcaag gtttatgtac
    20221 tttccggttg ccgcattttc tggattttct gcaagccagg ggatctctcc cagcagcggc
    20281 gcgggaatca tgcgggtgag cgtggtcata tattcagcgt gacgttttcc cggaggcgtg
    20341 acatcgttcg ccacccaacc cgccagagtc agcccggcgt gttgtatcgc ctgtgccgtc
    20401 aacatcgcgt gattaataca gccaagtttc acgccaacca ccagtattac cggcagttgt
    20461 tcctgtgtta cccaatccgc aaaagtgaaa gtgtcagaaa gcggcgtaaa ccagccgcca
    20521 gcgccttcca ctaacaccca gtcagcctgt tgttcaagcg cgcgtaatcc ggagctcatt
    20581 accgatgatt ctatcgacct gccctcttgc gcgctgatga tgtgcggtga ggtgggttct
    20641 gcgaaggtgt aaggatttac cgttgcgtaa tccagctgca ggctgctgtt gcgctgtaac
    20701 gccagcgcgt cactattacg taaaccctcc ggggtctttt cgctgccaga ggcgaccggt
    20761 ttataacctg ccgtccgata gcctgctgcc tttgcggctt gtaaaagtgc acaactggcg
    20821 acagttttcc ccacttcggt atccgttccg gtgacaaaat aacgtttact cacgaacaat
    20881 cactcccaaa aaaagatgat acgtcagagg atatcgcccc tgttgttgcg gccaggccag
    20941 ttgcaatcgc tgcaactgtg aacgcgttaa tattcgctgg tcgcgccctt catgaagatg
    21001 cgtggcaccg atgcctttca gcgaacgcat ggcactgagc gcatcatcaa accatagtgt
    21061 gatgggctga atacgatgtt gataatgcac gccgttcagc gactgttcga tttcatctgg
    21121 cggtaaaaag cgattagcat gcgggcgctc gtccaccgcc tgccacgcct gatgcagttc
    21181 gggtaacgat ccctgcacca gcgtggtaaa cgcgaccacg cctccggggc gcaccacccg
    21241 atacagctcg cggagtgccg ttgataaatt accgcaccac tgcaccgcga gattgctcca
    21301 tgcaagatcg aacgtcgcag tcgctaacgg cagggattcg atatctcccg ccagataatg
    21361 gtctgcagca tctttctggc gggcttgcgc aagcattggc ggcgagagat ctaacgccgt
    21421 cacctgcgcg tgacgctctc gccagtggcg gctcatccag ccaggcccac accccgcgtc
    21481 cagtacgtgg gggtatttac gctgtgggag cattgccagt aagacatcag cactctggcg
    21541 ctgtaactct gcgtgttgct catagtgcgc ggctgcccga ccaaatgccg ctgcaatggc
    21601 ttgtttatta accgttgcca tgcagcacct ccagcagacg gtcgatatcc tgcatttcat
    21661 gcgcggcggt tagcgttaag cgcagtcgcg cagtaccaga gggtacggtt ggcgggcgaa
    21721 tcgccgtgac ccagcagcct tgctggcgca gtttttctgc cagttgtaac gcacggctgt
    21781 tatcaccgac gatcaatggc tggatggcgc tgcatgaatc agcaagcgta aacggcaaat
    21841 cctgtactcc ggcacgaaaa cgcgtaatga gtgccgccag tttttcgcgc cgtgcatcac
    21901 cctcatcact gcgaatgacc gccagtgacg cacgtaatgc ctgcgcctga gcgggcggca
    21961 tactggtgct gtagataaga ttgcgggcga attgcagcag ataatccgcc accgtactgg
    22021 agcaaagcac cgcagccccg ctgacgccaa atcctttgcc aaaagtcact accagcaatt
    22081 ctggttttac cttttgcaac cagcaggtgc cgcgcccctg ctccccgata acgcccgtgc
    22141 cgtgggcatc atcgaccatc aaccagccat tgtgctgttg cgttacctgc tggatttccg
    22201 ccagtggcgc actatcgccg tccatgctga acacgccttc tgtcaccacc agttgctgcc
    22261 ccggacaggg ggaagcaagc agtcgcgcca gatgagtgac atcgttatga acaaaacggc
    22321 gaagctgcga cgggcttaaa ctggcagcct ccagcaatga ggcatggcta agccggtcag
    22381 cgacaatgcg gtcctctttt gccatcatcg cggcaatgac cgcctgatta gcggcaaaac
    22441 cagagataaa tagcagtgcc cgcgaatagc ccagccactc ggccagttct tcttccagcg
    22501 cctgatgcgc aacgctataa ccgctgacgt gaccggaacc gccgctaccg acgccaaatt
    22561 gctccgcacc ctgcttccag gcacggataa tttgcggatg atggcttaaa cctaaataat
    22621 cgttactgga aaagttcaga tactggcgat cgtccgccac cagccagcgt ccggctcctt
    22681 gcgccactgg ataacggcga cgcagggcat cggcagcacg ccgcgcatcg agcgccgcgt
    22741 tgattttctc ctgccagctc atagtgctgc cgcgttgtaa tattcgtcag tgtccggggt
    22801 catcagtgcc tgctccagac gctgctgttg ttcgttatcc cctgccagca cggcagtttg
    22861 ctgcggattt agccccagtt tgcgaaacag ttgcaggtct ttatcttctt ccggattggg
    22921 cgtggttagc agtttgcaac cgtagaaaat cgagtttgcg cctgccataa agcacatcgc
    22981 ctgagtctgt tcgttcatct gctcgcgtcc ggcagaaagg cgcacgtaag aggtcggcat
    23041 catgatccgc gctacggcaa tggtgcgaat aaaatcaaag gcatcgacat catcgttatc
    23101 tgccagtgga gtacctttca ccttcaccag catgttgatt ggcacgcttt ccggcggcgt
    23161 cggcaggttt gccagttgca gtaataatcc ggcgcgatct tttaccgttt cgcctaaacc
    23221 cacaatgcca cctgagcaga ctttgatccc ggcatcgcgc actttttcca gcgtatcaag
    23281 gcgttcctga taagtacgtg tggtgatgat attgccgtaa aactccggcg aggtgtcgag
    23341 gttatggttg tagtaatcca gcccggcatt cgcaaggcgc tgcgcctgag attcactcaa
    23401 cgtacccagc gtcatacacg cctccagccc catcgctttt actccctgca ccatttgttc
    23461 caggtatgac atatcgcgtt cgtggggatt cttccacgcc gcgcccatac agaagcgcgt
    23521 cgatcctgcc gctttcgctt tgcgcgccga ctccagcacc tgttcaactt ccatcaaccg
    23581 ctcggcttcc agcccggttt tgtagcgcga gctttgcggg cagtatttgc aatcttccgg
    23641 acaagctccg gtcttaatcg acagcaaggt gctgacctgc acctgacgag gatcgaaatg
    23701 ctggcgatgc acctgctgcg cttcaaacag cagatccagc aacggttttt caaataattc
    23761 tgtgacttgc gacaatgtcc agcgtgggcg gtgagccatg gggcttctcc aaaacgtgtt
    23821 ttttgttgtt aattcggtgt agacttgtaa acctaaatct tttcaatttg gtttacaagt
    23881 cgattatgac aacggacgat cttgcctttg accaacgcca tatctggcac ccatacacat
    23941 ccatgacctc cccgctaccg gtttatccgg tggcgagcgc cgaaggttgc gagctaattt
    24001 tgtctgacgg cagacgcctg gttgacggta tgtcgtcctg gtgggcggcg atccacggtt
    24061 acaatcaccc gcagcttaat gcggcgatga agtcgcaaat tgatgccatg tcgcatgtga
    24121 tgtttggcgg tatcacccat gcgccagcca ttgagctgtg ccgcaaactg gtggcaatga
    24181 cgccgcaacc gctggaatgc gtttttctcg cggactccgg ttccgtagcg gtggaagtgg
    24241 cgatgaaaat ggcgttgcag tactggcaag ccaaaggcga agcgcgccag cattttctga
    24301 ccttccgcaa tggttatcat ggcgatacct ttggcgcgat gtcggtgtgc gatccggaca
    24361 actcaatgca cagcctgtgg aaaggctatc tgccagaaaa cttgtttgct cccgccccgc
    24421 aaagccgcat ggatggtgaa tgggatgagc gcgatatggt gggctttgcc cgcctgatgg
    24481 cggcgcatcg tcatgaaatc gcagcggtga tcattgaacc gattgtccag ggcgcaggcg
    24541 ggatgcgcat gtaccatccg gaatggttaa aacgaatccg caaaatgtgc gatcgcgaag
    24601 gtatcttgct gattgccgac gagatcgcca ccggatttgg tcgtaccggc aaactgtttg
    24661 cctgtgaaca tgcagaaatc gcgccggaca ttttgtgcct cggtaaagcc ttaaccggcg
    24721 gcacaatgac cctttccgcc acactcacca cgcgcgaggt tgcagaaacc atcagtaacg
    24781 gcgaagccgg ctgctttatg cacgggccaa cttttatggg caatccgctg gcctgtgcgg
    24841 ctgcaaacgc cagcctggcg attctcgaat ctggcgactg gcagcaacag gtggcggcta
    24901 ttgaagtaca gctgcgcgag caacttgccc ccgcccgtga tgccgaaatg gttgccgatg
    24961 tgcgcgtgct gggggccatt ggcgtagtcg aaacaactcg tccggtgaat atggcggcat
    25021 tgcaaaaatt ctttgtcgaa cagggtgtct ggatccggcc ttttggcaaa ctgatttacc
    25081 tgatgccgcc ctatattatt ctcccgcaac agttgcagcg tctgaccgca gcggttaacc
    25141 gcgcggtaca ggatgaaaca tttttttgcc aataacggga agtcagcgtg agagtttctg
    25201 gctacacttt ctgcaaacta gaaaggaggg tttatgaaac tcatcagtaa cgatctgcgc
    25261 gatggcgata agttgccgca tcgtcatgtc tttaacggca tgggttacga tggcgataat
    25321 atttcaccgc atctggcgtg ggatgatgtt cctgcgggaa cgaaaagttt tgttgtcacc
    25381 tgctacgacc cggatgcgcc aaccggctcc ggctggtggc actgggtagt tgttaattta
    25441 cccgctgata cccgcgtatt accgcaaggg tttggctctg gtctggtagc aatgccagac
    25501 ggcgttttgc agacgcgtac cgattttggt aaaaccggat acgatggcgc agcgccgccg
    25561 aaaggcgaaa cccatcgcta catttttacc gttcacgcgc tggatgtaga gcgtattgat
    25621 gtcgatgaag gagccagcgg cgcgatggtc gggtttaacg ttcatttcca ctctctggca
    25681 agcgcatcga ttaccgcgat gtttagttaa tcactctgcc agatggcgca atgctatctg
    25741 gtatcactta aaggtattaa aaacaacttt ttgtcttttt accttcccgt ttcgctcaag
    25801 ttagtataaa aaagctgaac gcgaaacagc aaaacccaat aatatcaata tattacaatg
    25861 catttagtct aaaaaataga ctgcataatg ctacaaaaca caacatatcc agtcactatg
    25921 aatcaactac ttagatggta ttagtgacct gtaacagagc attagcgcaa ggtgattttt
    25981 gtcttcttgc gctaattttt tgtcaaacac attacgtcgt ttgctgaatt tacaaccatg
    26041 ctcagtatct ctataagcgc agagaaatga tgcagtggtg ggtgggctgg cttgatgaaa
    26101 aggtggggta ctccacctga accactatcg gaatagcaca aagccttgca atccagtgca
    26161 aagttttgtg tgcctccgtt tgccccacca atatatattc agcacaagaa acacagctaa
    26221 agcaatggat gcagtcttac ctgttggaat cccagactca ctgtattgtc atcatgaata
    26281 agagctgagt cgtgcgggta ggaggttcta cggggcatcc caatctcttc gtggaattta
    26341 ttttctgaaa gcacctcctc tgaaaaagcc cccaacccaa cagtcctggc ttcttcgagt
    26401 aaaagtggag agtatttttg tgattctgct cgggaactct caactttcaa acgctcccca
    26461 tttaaattgt ggaaaacatg gagcaactca tgaaaaacaa tagtcgcatg aaagtccacc
    26521 acgctaatcc cctccccaca gggatattca actgcattca gattacagtg aaaatcggaa
    26581 ccagtccccc gatggttctc agcatctata tctctatgtg ccataactcc tagtctggaa
    26641 gagttgaggt gtattaccac tgtttctgat ttaagtcggg atattgattc aatagcattc
    26701 aggagtacac gccctgtctc tgtagagcca atcttatcga gtgctgcttc aatctgctga
    26761 tatatattat gatcagtccc ctggaaacga agttgcggat tattttttat ccattgaacg
    26821 ctcgatgctt ttacaagaga ctcgatatct gtatctgaca ttgagctcga ccttgatgac
    26881 tgatgtaata ccaagttgag ggacgtaggg cgcatcagga ctcctgagaa tgctacttat
    26941 tagcagcctg tttaccactc tgtgaaaatg atttttttaa aagtgaccgt caaggcggtc
    27001 accaaacccg ataataaagc ggatcatagc catttgccag tcctcagcga cattgcccat
    27061 ttccgtgatc cgccaagaat tgccaaccac acaatttgtt accaactcca tccatgcaac
    27121 aaatccacta cactggataa aattactaaa ttttacttaa taccacacta ataagatctt
    27181 gctttccgcc atgataagat tgcattattt gactttcact ccatgaacgg tcggaaacat
    27241 tataagatga tatatctatt ggattaaact cattcttcgc gcggtcatat aagacatttg
    27301 tctctgttgt atcgacaaaa agaattcctt tttgctccag tctatcaaac atatcgtaaa
    27361 tagcatgctc agcctgtgct ggcaaggacg aaatatttaa aagcgattct ccatttattt
    27421 tatccattct aataccaata atatcaccat tattgccata tattttttct gcactcccgg
    27481 caccataata ttggttgaag caacgaactt cgcttgtcac ctcttcattg ctttgagatg
    27541 tagtaaacat cttcaggact tttgttgcat cctcagcatc ttcatatact acagcgttcc
    27601 cccctttgcc aataacatta ccaggtactg acaacctgtt atagtccaca ctcggtaact
    27661 ctggcggtgc ataatcaaca ggaggtaaat caggtctgtt cgaatgaaca atgcctcttt
    27721 ccatagcagc gctcactggt gatgcattca gcatgacctc aattttcctg ctaatctccc
    27781 ccttaggcca tcccagcctg tgcaacagat tagtaaaaca accactatga ctttctcttg
    27841 taacgcaaaa cttattatcg gtggcaacaa cacgatatgt tctgttgcca acctttactt
    27901 gcgccccatt atcagaatga acggcagcat cccttacaga ggataaaaca cgattatcag
    27961 gcgaagtcag gtttctggtt aaagaattcc atgaacatcc caaatttaca gaatatggcg
    28021 ataacataca tttcaacctt caaaataaac ccatctaaat ttccttaaca aacatccccc
    28081 taacatggca acaaaaatta gaaatgaact cttgcttttt gtatctggca actaattcat
    28141 ccttccagca tcccatcaaa cgaacgttta cgaactataa cgcaggtatt accggtcact
    28201 cgatatctgg agatacagac gtatcaacat gattgagcgg catacgatac tgcttccatg
    28261 ccgccagtaa cgatgtctgc tcatccgtcg caatatctaa atctgcagca tcctgaagtg
    28321 gtgcgatata ctcactgatt acaagtacca gaccgataga ttaaaaaaaa cacaataaca
    28381 cgctgcattt aaaacaaaaa attttaaata tgatttaata caagctttaa gcctttaatt
    28441 atcagtttta tttattttcc agcaattgat taatcaattg tttatataca aattattcct
    28501 tttctttctc tcacgcaaaa ttcacaaaat attgttgctg caatggacct gtacaatagt
    28561 gaatcacggt acttttctgt gcacaacgta ctgcagtttc tttacgattt atagctgtac
    28621 tgttgaatta tgtacaattt gaatccatgc atgacgtgtg atgcctattg tgcatttttc
    28681 cgagcctctt tttactgggc cgaagccgat gatgctgata gtctcgttcc ggccatgttc
    28741 acttactaca gtaaaattag gggaattgcc tttacataca atgttgcacg attacccatc
    28801 atcaactccg ttaaaatgta tgaatagtaa ccttatgtca ctgcaaagac gaatcattgc
    28861 tgattgtgtt tgtccacatc cccaaatgta aaaatataag gggcgccatg atacagttgg
    28921 atctgatttg catcctgtaa attatcgttg atagccaatc caggaaaatc atgttgctgg
    28981 ataaatccgt atcctatgtt agacacagcg gaatactctg tgaaatcagg actcgcctca
    29041 tatcggtcac tgatcgtacc cagcctttcg aagaaagccc tctcattctc ttcatgcctc
    29101 atggcagcct gtcgaagggc attcaggtta cgtagcctaa gatgagcttc acgttctggc
    29161 tctgcatatc ctttgaagtc gggaacactc catcccagtt cttgagcgac acgacgtgcg
    29221 agaatctcgg tgggtcctaa ctctatatta ctatctccag atggatcgct agatccagta
    29281 acatgatgaa taatctcgtg aatcagtcct tcctgccacg atggcatctc ataagagtca
    29341 gtatctggcg caacactaaa actgacataa ggctcttcgt tatcgttttc tcctgcttcg
    29401 caaatgggga aaattggctc ttgcccgaca tcatattcat ataattcgtt acaggttagt
    29461 gattgaatat catcaatttt gacagaagat tcttcgttaa gctcgtattc gtttctgtat
    29521 ttaatgcaac caatgtgtac cttctcatta tgaatgccat agcttacggc atcacgaaat
    29581 gtctgtgatc ggctcaaagc atcaagtaca gtgttgccga tcatatcgac tgtatgctga
    29641 tcgataagcc tgctacggct gtcatggacg gcgcgtatta cactttcgta tacgttgctt
    29701 aaatctgctg cggaaagtgg tattcgctta cctatatcca aaacgtaatc cgcataggca
    29761 ttttcagcac gattaggagc aatgggagca gagtatcctg ccggggcgga aaagttgaag
    29821 ttggactgta atgagggaat tttcatgttt atatctaata ccctgcaaat catttattac
    29881 aacaaaataa actatatcaa attcatttac catgaactgc atgtatactg acttgtgttc
    29941 atgattatat tagggtggtt aaactcaata aagtcacaaa aatgattata attatgtaat
    30001 ggtgtaaaat taaaatattt cttaactcct ttccccaaac catcataata tggatgagca
    30061 tctactttac tatgcataaa agaaagtccc tcaagtagag caggatgatt actacggtta
    30121 acaattattg cactattttc aatatttaca ctatcattac gacgatccac atgcattgaa
    30181 attccatcag gagcatatat tgtaccaagc ttacctgtaa gtatcatatc catatcaaga
    30241 tagatacacc cctccccgaa tgaaataccg tgattctttg tattatatgt gcacctgaat
    30301 atctctcctg cttttaataa ggctaaattt ctgaagaaat caaaccatgc atgatttctt
    30361 ttctttgcat acatagaaat caaagaatcc tgcgcctttg aaatttccct cagctctttc
    30421 tctaacagat tcaacaagta ttcatctctt ttgtctt
//
LOCUS       DAZKIP010000049        29806 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZKIP010000049 DAZKIP010000000
VERSION     DAZKIP010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.049"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            76..1674
                     /gene="rtcR"
                     /locus_tag="ACHOUK_002429"
     CDS             76..1674
                     /gene="rtcR"
                     /locus_tag="ACHOUK_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFP9897867.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCICQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSLYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVADGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFASSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(1656..2414)
                     /locus_tag="ACHOUK_002430"
     CDS             complement(1656..2414)
                     /locus_tag="ACHOUK_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFP9897868.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDTPPPVSVMQVLTDHHIQLELC"
     gene            complement(2431..3261)
                     /gene="glpG"
                     /locus_tag="ACHOUK_002431"
     CDS             complement(2431..3261)
                     /gene="glpG"
                     /locus_tag="ACHOUK_002431"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFP9897869.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3306..3632)
                     /gene="glpE"
                     /locus_tag="ACHOUK_002432"
     CDS             complement(3306..3632)
                     /gene="glpE"
                     /locus_tag="ACHOUK_002432"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFP9897870.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRNNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3822..5327
                     /gene="glpD"
                     /locus_tag="ACHOUK_002433"
     CDS             3822..5327
                     /gene="glpD"
                     /locus_tag="ACHOUK_002433"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFP9897871.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAVSDLGEDFGHEFYETE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(5542..6144)
                     /locus_tag="ACHOUK_002434"
     CDS             complement(5542..6144)
                     /locus_tag="ACHOUK_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9897872.1"
                     /translation="MMNIIRLYIYPLFLGFVIVPLLVWPTVVALAACVITLSFLGEIL
                     FSIPLMHRRISLLQLQLWLSAEYALFFCAMVGVGWQFARRTPPELKSRLHCWLVFAPV
                     YFWLLLWNIIFYVAPAQIALLENMRSFFLTVVWLPLNFSPFWSQQWIDFVGPISAQLG
                     FALGYYCQWRSKNRSQIRKWSEWGTCLSFVTLGMMAWALR"
     gene            complement(6144..7136)
                     /locus_tag="ACHOUK_002435"
     CDS             complement(6144..7136)
                     /locus_tag="ACHOUK_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP9897873.1"
                     /translation="MNKFTGVLLLGTALLAGCVDREGYYNSVREEESHGLTSLRGQPA
                     LRYSDDWSRWPRVYGATALYPLYASAYYKLVPEPKDKDRTSLAWQAYGLQQTRTAEAY
                     DSLIKGSATVIFVAQPSEGQKKRAEEAGVKLKYTAFAREAFVFIVDINNPVNSLSEHQ
                     VKDIFSGKTSRWNKVGGSDEHIKVWQRPEDSGSQTIMKGLVMQDTPMLPAKKSTVIDL
                     MGGLITEVADYQNTPSSIGYTFHYYVTRMNDNMLKMRKQIKLLAINGVAPTEENIRNG
                     TYPYIVDAYMVTRENPTPETQKFVDWFISQQGQQLVEDVGYVPLYEASPESSGQ"
     gene            complement(7162..8670)
                     /locus_tag="ACHOUK_002436"
     CDS             complement(7162..8670)
                     /locus_tag="ACHOUK_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP9897874.1"
                     /translation="MMPNRKWILTSLIMTFFGIPILAQFLAAVVAMLGVGLTGIIEVC
                     NILITPTIYLLLNVFMLTLGAIIIFFSGRVWAGDSAPENREIAVWRQCFFLLPALLTL
                     VGWIITLHLADYQFRQMGAGWLANLMLPWLGVFLVSLVGGEYWWMVIIPVGAHISFSL
                     GYAWPTRYPLSGTSGLRCRNLLLFLLLLLGIVAGYQAHLYKQQNPGVGVRENIDIRAW
                     RPDKLNNRLTPLRGKPQIQFRQNWPRIDGATAAYPIYASAFYALSVIPEDFHVWEYLE
                     NSRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKKVRMISP
                     QETEVASVMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIRLLAINGITPTAENI
                     RNGKYAYIVDAFMVTRENTTSETQKLVEWFLTPQGQSLVEDVGYVPLYLTME"
     gene            complement(8795..11242)
                     /gene="glgP"
                     /locus_tag="ACHOUK_002437"
     CDS             complement(8795..11242)
                     /gene="glgP"
                     /locus_tag="ACHOUK_002437"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFP9897875.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADLDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(11261..12694)
                     /gene="glgA"
                     /locus_tag="ACHOUK_002438"
     CDS             complement(11261..12694)
                     /gene="glgA"
                     /locus_tag="ACHOUK_002438"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFP9897876.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(12694..13989)
                     /gene="glgC"
                     /locus_tag="ACHOUK_002439"
     CDS             complement(12694..13989)
                     /gene="glgC"
                     /locus_tag="ACHOUK_002439"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFP9897877.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(14007..15980)
                     /gene="glgX"
                     /locus_tag="ACHOUK_002440"
     CDS             complement(14007..15980)
                     /gene="glgX"
                     /locus_tag="ACHOUK_002440"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFP9897878.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGVGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(15977..18163)
                     /gene="glgB"
                     /locus_tag="ACHOUK_002441"
     CDS             complement(15977..18163)
                     /gene="glgB"
                     /locus_tag="ACHOUK_002441"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFP9897879.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPLNEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(18436..19539)
                     /gene="asd"
                     /locus_tag="ACHOUK_002442"
     CDS             complement(18436..19539)
                     /gene="asd"
                     /locus_tag="ACHOUK_002442"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFP9897880.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            19731..20324
                     /gene="yhgN"
                     /locus_tag="ACHOUK_002443"
     CDS             19731..20324
                     /gene="yhgN"
                     /locus_tag="ACHOUK_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFP9897881.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(20370..21665)
                     /locus_tag="ACHOUK_002444"
     CDS             complement(20370..21665)
                     /locus_tag="ACHOUK_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFP9897882.1"
                     /translation="MDADSSNSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASKYKPLSNSEWGKFALWLKHQRISPAELLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAMEALKHQWLPVQVKPNQDMQSANSRLVENGASWSAEQAENVTIR
                     LPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAYTPVDFYQLFVAELAILAKESIS
                     IERLASCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(21572..23667)
                     /locus_tag="ACHOUK_002445"
                     /pseudo
     CDS             complement(21572..23667)
                     /locus_tag="ACHOUK_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
     gene            complement(23807..25147)
                     /gene="gntU"
                     /locus_tag="ACHOUK_002446"
     CDS             complement(23807..25147)
                     /gene="gntU"
                     /locus_tag="ACHOUK_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFP9897883.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHLFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(25151..25678)
                     /gene="gntK"
                     /locus_tag="ACHOUK_002447"
     CDS             complement(25151..25678)
                     /gene="gntK"
                     /locus_tag="ACHOUK_002447"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFP9897884.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(25817..26812)
                     /gene="gntR"
                     /locus_tag="ACHOUK_002448"
     CDS             complement(25817..26812)
                     /gene="gntR"
                     /locus_tag="ACHOUK_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFP9897885.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(27036..27731)
                     /gene="yhhW"
                     /locus_tag="ACHOUK_002449"
     CDS             complement(27036..27731)
                     /gene="yhhW"
                     /locus_tag="ACHOUK_002449"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFP9897886.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASISDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(27854..28891)
                     /locus_tag="ACHOUK_002450"
     CDS             complement(27854..28891)
                     /locus_tag="ACHOUK_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFP9897887.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLNEVLNDPDVKLVVVCTHADSHFEYAKRAMEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            29225..29713
                     /gene="yhhY"
                     /locus_tag="ACHOUK_002451"
     CDS             29225..29713
                     /gene="yhhY"
                     /locus_tag="ACHOUK_002451"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFP9897888.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYYNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDARWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYFMAR
                     VK"
BASE COUNT         7418 a         8039 c         7314 g         7035 t
ORIGIN      
        1 acgtccgtaa acggagattt cccgcaaagc caatttaccg ataatgaaat atcgtctttt
       61 ataaggatat ctaagatgcg taaaacagtg gcttttggct ttgtcggaac agtgctggat
      121 tatgccgggc gcggcagtca gcgctggtcg aaatggcgtc cgtcactctg tatatgccag
      181 caagaatcgt tagtcatcga tcgactggaa ttattgcatg acgcccgctc gcgctcgcta
      241 tttgaaacgc ttaaacgcga tatcgccagc gtttcgccag aaaccgaagt ggtgagcgtt
      301 gagattgaac tgcataaccc gtgggatttc gaagaggttt acgcctgcct gcatgatttc
      361 gcccgtggtt acgagtttca gccagaaaaa gaagactatt taattcacat caccaccggc
      421 acccacgtcg cgcagatttg ctggtttctg ctggctgaag cgcgttacct gcccgcccgg
      481 ttgatacaat cttcacctcc gcgcaaaaaa gaacagccgc gcggcgcagg tgaagtgacg
      541 attatcgatc tcgatttaag cctttataac gccatcgcca gccgctttgc cgaggaacgc
      601 cagcaaacgc ttgattttct taagtccggt attgccacgc gtaaccccca cttcaaccgc
      661 atgattgagc agattgaaaa agtagcgatt aaatcccgcg caccgatcct gctaaacggc
      721 ccaaccggtg cgggcaagtc atttctggcg cgacgtatat ttgaattaaa acaggcgcgg
      781 catcagttta gcggcgcgtt tgtggaagtg aactgcgcaa ccctgcgcgg cgataccgcc
      841 atgtcgacgc tgtttggtca tgtgaaaggc gcgtttaccg gggcacggga atctcgtgaa
      901 ggattattac gcagcgccaa cggcggaatg ttgtttcttg atgagattgg cgaactgggc
      961 gcagacgaac aggcaatgct gctgaaagcc attgaagaaa aaacctttta tccgtttggc
     1021 agcgatcgcc aggtgagcag tgattttcag cttatcgccg gaacggtgcg tgatttgcgc
     1081 cagctggttg ccgatggcaa attccgcgaa gatctatacg cgcggatcaa tctctggacc
     1141 ttcaccctgc cggggctacg ccagcgtcag gaagatattg aacctaacct ggattatgaa
     1201 gtggagcgcc acgcttcact caccggcgac agcgtgcgtt ttaacaccga agcgcggcgc
     1261 gcctggctgg cctttgcgac atctccccag gcaacatggc gcggtaactt tcgcgagctt
     1321 tctgccagcg tcacgcgaat ggcaaccttt gcctctagcg gacgcattac tctggacgtg
     1381 gttgaagatg agataaaccg tctgcgctat aactggcagg agagccgccc ctccgcgctt
     1441 acggcgttgc tgggcgcaga ggcagaaaac atcgatctct tcgaccgtat gcaactggaa
     1501 cacgttatcg caatctgccg ccaggcaaag tcgctttccg cagccggacg ccagcttttt
     1561 gacgtttcgc gccagggcaa agccagcgtc aatgacgcgg atcggctacg caaatacctg
     1621 gcgcgttttg gtctgacgtg ggaagccgtg caggatcagc acagctccag ttgaatatgg
     1681 tggtccgtca gcacctgcat cacgcttact ggcggcgggg tgtcggtgta gacggcatct
     1741 accatgctga tgctacccat attgaccatc gcgttacggc caaatttcga gtgatcgaca
     1801 accagcataa cgtggcgcga gttctcaata atggcgcgtt tggtgcgaac ttcgtggtaa
     1861 tcgaactcca gcagcgagcc gtcgctatcg atgccgctta tccccagaat gccgaaatca
     1921 aggcggaact gggagataaa atcgagcgtc gcttcgccaa tgatcccgcc atcgcggctg
     1981 cgtaattcgc caccggcgag aataatacgg aaatcctctt ttaccatcag cgtgttagca
     2041 acgttaagat tgttggtgac aatgcgcaaa ttgctgtgat tgagcagcgc gtgcgccacc
     2101 gcttccggcg tggtgccgat atcgataaac agcgtcgagc cattggggat ttgctccgcc
     2161 actttgcggg cgatgcgctc tttttcttcg gtctgggtgg ctttacgatc gtgccacggc
     2221 gtgttaaccg aactggaagg cagcgccgca ccgccatgat ggcgcaggat caggttttgc
     2281 tccgccagct cattgaggtc gcggcgaata gtctgcgggc tgacggagaa gtgctccacc
     2341 agctcctcgg tactgacata accctgctgt ttaaccagtt cgataatacc gttgtgacgt
     2401 tgtgtttgtt tcatttctaa atccctggaa ttattttcgt tttcgcgcat tgagcgaatc
     2461 aacaaaagcc atcgctaaac ccacggctaa cccggcgatg tgtgctccgt tcgccatcga
     2521 catcccaaac aaatcaaacc atccggcgac aatccagatc agcgcaaaga taattaaccc
     2581 acgttgcagg taaatgccac tttgcggatc gcgttcgcca cgtagccaga cgtagcccat
     2641 cagcgcatac accacgccag aaagcccgcc aaaccacggc ccgctgaatt tttgctgcac
     2701 atagccgctt aacagggcgc tgataagcgt aatgacaatt agcttaccgc taccgaggcg
     2761 tttttccacc gcaccgccga gataccacca ccagagcagg ttaaagagga tatgcatcag
     2821 cgagaagtgc attaacgcgt gggtgaagta acgccagaac tcaaatttca gtgctggatc
     2881 gaatggccaa gccagccata acatcacttc ctgatcgccg agaatttgca tggcaataaa
     2941 caccaccacg caggcgatca tcatcaccca ggtcaccgga cctgcgcgtt cacgcaaggc
     3001 ggcaaagaaa ggataacggc gataatgcag gccactgccg gtatggcctg cctgccagct
     3061 cgccgccaga taacgcggat ctgccgggtt ttcgagaaaa cgcgccagct ccgcccgtac
     3121 gcgctcggcc tgggactcat ccgccagcca gacatcgctt tggttatgtt gttgaatcgt
     3181 gaggataaca ccctgcgtcg ccatgtaatc aacaaacgcc tgcgccacgc gggggttagc
     3241 aaaagaggta atcatcaaca tcgttgctgt cgcttattcc acacaaaagg ggacagtata
     3301 aagcgttacg cgccgtacgc cacctctgcg ggaaactgac gttgccaggc ttcaaagccg
     3361 ccgtcaatgc tatagaccac atcgtagccc tgttgcagca gatactgcgc cgcgcctttg
     3421 ctgctattgc cgtgataaca catcaccatc accggagtgt caaagtcgtt attacgcata
     3481 aaagcgccca gcgtgtcgtt ggttaaatgg aaagcctgca ccgcatgtcc cattgcgaaa
     3541 ctctgtggat cgcgaatatc gaccagcacc gcctcttttt cctgcaactt ctggtgcgcg
     3601 tcggcaacgt taatacattc gaactgatcc atgcgtctct ctttctttac aaacaagtgg
     3661 gcaaatttac cgcacagttt acgtcgaagc ggcagataaa cgccataatg ttatacatat
     3721 cactctaaaa tgttttttca atgttaccta aagcgcgatt ctttgctaat atgttcgata
     3781 acgaacattt atgaacttta acgaaagtga atgagggcag catggaaacc aaagatctga
     3841 ttgtgatagg tggcggcatc aatggtgctg gtatcgcggc agacgccgct ggacgcggtt
     3901 tatccgtgct gatgctggag gcgcaggatc tcgcttgcgc gacctcttcc gccagttcaa
     3961 aactcattca cggtggcctg cgctaccttg agcactatga attccgcctg gtcagcgagg
     4021 cgctggctga acgtgaagtg ctgctgaaaa tggccccgca tatcgccttc ccgatgcgtt
     4081 ttcgcctgcc acatcgtccg catctgcgcc cggcgtggat gattcgcatt ggtctgttta
     4141 tgtacgatca tctgggtaaa cgcaccagcc tgccgggatc aactggtttg cgttttggcg
     4201 caaattcagt gttaaaaccg gaaattaagc gcggattcga atattccgac tgttgggtag
     4261 acgatgcccg tctggtgctc gccaacgccc agatggtggt gcgtaaaggc ggcgaagtgc
     4321 ttactcgtac tcgtgctact tctgctcgtc gcgaaaacgg cctgtggatt gtggaagcgg
     4381 aagatatcga taccggcaaa aaatatagct ggcaagcgcg cggcctggtt aacgccaccg
     4441 gcccgtgggt gaaacagttc ttcgacgacg ggatgcatct gccttcgcct tatggcattc
     4501 gcctgatcaa aggcagccat attgtggtgc cgcgcgtgca tacccaaaag caggcctaca
     4561 ttctgcaaaa cgaagataaa cgtattgtgt tcgtgatccc gtggatggac gagttttcca
     4621 tcatcggcac caccgatgtg gaatacaaag gcgatccgaa agcagtgaag attgaagaga
     4681 gtgaaatcaa ttacctgctg aaagtgtata acgcgcactt taaaaaacag cttagccgtg
     4741 acgatatcgt ctggacctac tccggtgtgc gtccgctgtg tgatgatgag tccgactcgc
     4801 cgcaggctat tacccgtgat tacacccttg atattcatga tgaaaatggc aaagcaccgc
     4861 tgctgtcggt attcggcggt aagctgacca cctaccgaaa actggcggaa catgcgctgg
     4921 aaaaactaac gccgtattat cagggtattg gcccggcatg gacgaaagag agcgtgctac
     4981 cgggtggcgc catcgagggc gaccgcgacg attatgccgc tcgcttacgc cgccgttatc
     5041 cgttcctgac cgaatcgctg gcacgtcatt acgcgcgtac ttatggcagc aacagcgagc
     5101 tgctgctcgg caatgcggga gcggtaagcg atcttgggga agatttcggt catgagttct
     5161 acgaaacgga gctgaaatat ctggtcgacc acgagtgggt ccgccgcgcc gacgatgccc
     5221 tgtggcgtcg cacaaaacaa ggcatgtggc tgaatgcgga tcaacaatct cgtgtgagtc
     5281 agtggctggt ggaatatacg cagcagaagt tgtcgctggc gtcgtaaatt aacgtaaggt
     5341 gatcaggtca gatttcaatc tggcctgaga ctgatgacaa acacaaaatt gcctgatgcg
     5401 ctacgcttat caggcctacg tggtttatgc aatatgttga atttgcatga ttttgtaggc
     5461 ctgataaggc gttcacgccg catcaggcat gaacaaagcg ccacgttgtc aacaatctga
     5521 cctacttttt gattcaggga attaccgcaa agcccacgcc atcataccca acgtaacaaa
     5581 acttaagcac gttccccact cactccactt ccgtatctgg cttctatttt tgctacgcca
     5641 ctggcaataa tatcccaacg caaaaccaag ctgtgcactt atcggaccga caaagtcgat
     5701 ccactgttga gaccaaaatg gcgaaaaatt cagtgggagc cagacaactg tcagaaagaa
     5761 actgcgcata ttttccagca aagcaatttg cgctggcgcg acataaaaaa tgatattcca
     5821 caggagcaac cagaaataaa ctggtgcaaa taccagccaa caatgcagtc tgcttttcaa
     5881 ttcaggtggc gttctgcgcg caaattgcca accaacgccc accattgcac agaagaaaag
     5941 cgcatattca gccgataacc atagttgtaa ttgcagaagg gaaatacgcc tgtgcatgag
     6001 cgggattgag aacagtattt ctcccagaaa actcagcgtg attacacagg cagccagcgc
     6061 aaccaccgtc ggccacacca acaacggtac aataacaaag ccgagaaata acggataaat
     6121 ataaagtctg attatattca tcattattgt cctgatgatt cgggggatgc ttcatacagc
     6181 ggcacatacc ccacatcctc taccaactgt tgcccctgct gacttataaa ccagtcaaca
     6241 aatttctgcg tttccggcgt gggattttca cgcgtcacca tataggcatc cacaatgtat
     6301 ggataagtgc cgttgcggat attttcctcg gtaggcgcaa cgccatttat agccaaaagt
     6361 ttaatctgct tgcgcatttt gagcatattg tcattcatac gagtgacgta atagtggaag
     6421 gtgtacccaa tggaagatgg cgtgttttga tagtcggcaa cttcagtaat taaaccgccc
     6481 ataagatcaa tcacagtgga ttttttagct ggcagcattg gggtgtcttg cataaccaac
     6541 cccttcataa tcgtttggct tccagaatct tcagggcgct gccagacttt tatatgttcg
     6601 tcactaccac ctactttatt ccagcggcta gttttgccgc taaaaatatc tttaacctgg
     6661 tgctcagaga gagaatttac cgggttatta atatcaacga taaagacaaa ggcttcgcgg
     6721 gcgaaagcgg tatatttcag tttaacaccc gcttcttctg cacgtttttt ctgtccttcc
     6781 gacggttgtg caacaaaaat aaccgtcgcg gaacctttaa tcagactatc gtaggcttca
     6841 gctgttcggg tttgctgcaa accatacgcc tgccaggcca gcgaggttcg atccttatct
     6901 tttggctcgg gtactaattt ataatacgcg gaggcataca gcgggtataa ggctgtagcg
     6961 ccgtacactc tcggccatct tgaccaatca tcgctgtaac gtaatgcagg ttgcccccgc
     7021 agagacgtca gtccatggct ctcttcttcc ctgacgctgt tatagtaccc ttcccggtcg
     7081 acacatcccg ccagtaacgc cgtgcctaat agtaaaacac cggtgaattt attcatctcg
     7141 ctgattcctt tctctcgagc gttattccat tgttagatac agcggcacat atcccacatc
     7201 ttctaccaga ctctgcccct gcggcgttaa aaaccattcg accagttttt gtgtttctga
     7261 cgttgtattt tctctcgtca ccataaatgc atcgacgatg tacgcatatt tgccgttgcg
     7321 aatgttttcc gccgtcggtg taataccgtt aatcgctagc aatcttatat ttttatcagc
     7381 attcatttgc gtcgcgtaat agcggaaggt atagcctatt gcgttgtttg tattacggta
     7441 ttcggcaacg actttaatca ttccctccat cacgcttgcc acttccgttt cctgcggcga
     7501 gatcatgcgg acttttttca tgacctgtga ttgcatcact gtctggctgc cagagtcttc
     7561 cgggcgctgc caggtctgga tctcctgatc gttaccgcca acagtacgcc aattggtaat
     7621 tgcaccactg aaaatgtcac gcacctgttg ttcagtcagg gaattaaccg gattatccgc
     7681 attgacgatg aaaacaaatg cttcacgggc aaatggcgtg tatagcaaag tgacgcccga
     7741 ttcctcagcg cgttttttct gcccgccgga gggttgcgcc acaaaaataa tatcggcatc
     7801 acctttaaca atccggttat atgcatcggg ggtacgagag ttctccagat attcccaaac
     7861 gtgaaaatcc tctggtatta cacttaatgc ataaaatgca gaagcataaa ttgggtacgc
     7921 agccgtggcg ccatcgattc gcggccagtt ttgcctaaac tgaatttgcg gtttgccacg
     7981 cagcggcgtc agtcgattat tgagtttatc gggtcgccag gccctgatat caatattttc
     8041 gcgtacaccg acaccaggat tttgctgctt atataaatgg gcctgatacc cggcgacaat
     8101 accaagtaag agaagtagaa acaggagtaa gttacggcaa cgtagtccgg acgtgccgga
     8161 taaaggatat ctggtcggcc aggcgtatcc cagcgaaaaa ctgatatgcg ccccaacggg
     8221 aataatgacc atccaccagt actcaccacc cactaatgag actaaaaaaa cgcccaacca
     8281 gggaagcata aggtttgcca accaaccagc tcccatctgg cgaaattgat aatctgccag
     8341 atgtagcgtg attatccagc caaccagggt taatagcgcg ggtaagagaa aaaagcattg
     8401 ccgccagacg gctatttctc tgttttctgg cgcgctatca ccggcccaca cgcgaccaga
     8461 gaaaaatatt attatcgccc ccagcgtcag cataaagacg ttgagtagaa gataaattgt
     8521 tggcgtgata aggatattac aaacttcaat aataccggta agcccgacac ccagcatggc
     8581 aacaaccgct gccaaaaatt gcgccagtat ggggatgccg aaaaaagtca ttatcagcga
     8641 ggtcaaaatc cattttctat ttggcatcat tctgtccatt cttcctgaat gacggaatta
     8701 tactccccga gaaccctcgc cccctctgga cacttttccg aaataaaggc gaaaaaaacg
     8761 ggaccttgtg gccccgttct gttttttagc gaacttacaa tctcaccgga tcgatatgcc
     8821 agatatgatc agcgtactct ttgatagtac ggtcagaaga gaagtagccc atattggcaa
     8881 tgttcagcat cgcttttgcg gtccactctt cctgaagctc gtagagttca tcgactttat
     8941 cctgacaatc gacatagctg cgataatccg ccagtacctg gtagtgatcg ccgaagttga
     9001 tcagcgaatc aaccagatcg cgatagcgac ccggatcttc cggactgaat acaccgctgc
     9061 cgatttgcgt cagcacctga tgcagctcct catctttctc gtagtattca cgcggtttgt
     9121 agccctgacg acgcagttct tccacttctt ccgctgtgtt accaaaaata aagatattgt
     9181 cagcaccgac atgatccagc atctcgacat tcgcaccgtc caacgtaccg atagtcagcg
     9241 caccgttaag cgcaaacttc atgttactag tgccggaagc ttccgtccct gccagcgaaa
     9301 tctgttcaga cagatctgcc gccggaatga tcaactgcgc caggctaacg ctgtagttcg
     9361 ggatgaacac gactttcagc ttatcgccaa tctgcggatc gttgttgatc actttcgcta
     9421 cgtcattgat caaatggata atatgcttcg ccatgtaata ggccgaagcc gccttaccgc
     9481 caaaaatatt cacgcgcggt acccacttcg catccaggtc ggccttgatg cggttatagc
     9541 gggtaatcac atgcaacaca ttcatcaatt gacgtttgta ttcgtgaata cgtttgattt
     9601 gcacatcgaa caacgccttt ggattcacca ccacattcag ctgctgggcg atatactccg
     9661 ccagacgctt tttattctcc agcttcgcct gatgcacagc gtgattaacc attgggaaat
     9721 cacagtgttg ttgcagctca ttaagcaggc taaggtcggt gcgccagtta cggcccaggt
     9781 gttcgtccag cacggctgaa agcgatgggt tcgctaccgc cagccagcga cgcggcgtca
     9841 caccgttggt gacgttggtg aaacgacccg ggaagatttt cgcaaagtcg gcaaacaacg
     9901 attgcaccat cagattagag tgcagttccg atacaccgtt aactttgtgg ctcacaacaa
     9961 ccgccagcca ggccatacgc acacgacgac cgttggattc atcaatgatc gacgcccgtc
    10021 ccagcagatc ggtatcgttc ggatactgtt cctgcaaggt tttcaggaaa tagtcgttga
    10081 tttcaaagat gatctgcagg tgacgcggca gaattttacc cagcatatca accggccagg
    10141 tttccagcgc ctcgctcatc agcgtgtggt tagtgtagga gaagacctga caacacacct
    10201 caaacgcgtc gtcccagcta aattggtgct catcgatcag cagacgcatc atctcaggaa
    10261 tcgacagtac cggatgggta tcattgagat gaatcgcgat tttatccgcc aggttatcgt
    10321 aggttttatg caactgataa tggcggctta aaatgtcctg aatggtcgag gaaaccagga
    10381 agtattcctg acgcaggcgc agctcacgcc cggagtaggt ggagtcatcc ggatacagta
    10441 cgcgagatac gttctcggag tggtttttat cttccactgc cgcgaagtag tcaccctggt
    10501 tgaatttacc gaggttaatt tcgctactgg cttgcgcact ccacaaacgc agcgtgttgg
    10561 tcgcgtcggt gtcgtaacca gggattatct gatcgtaagc gactcccaga atctcttcgg
    10621 tttcaatcca gcgcgttttt ttaccttcct gctgaatgcg accaccaaaa cggactttat
    10681 agcgcgtgtt gtggcgtttg aattcccacg ggttaccata ttccagccag tagtcaggcg
    10741 actctttctg gctaccgtta acgatgttct gcttgaacat accgtagtca tagcggatgc
    10801 cgtaaccgcg ccccggcaac cctaacgtcg ccagagaatc gaggaagcaa gccgccagac
    10861 gtcccaggcc accgttaccg aggcccgggt cattttcttc atcaatcagc tcttcgagat
    10921 ttaaccccat cgcttccagt gcgccctgta catcttcgta aattcctagc gacaacatgg
    10981 cgttggagag cgtacggcca atcaaaaact ccatcgacag gtagtaaacc tgacgagttt
    11041 cttgcgacaa ctgggcacgg tttgaacgta accagcgctc cacgagacga tcgcgcacag
    11101 caaataacgt tgcgttcagc cattcatgtt tattggcgac gaccgggtcc tttccaatcg
    11161 taaacatcag cttgtaagcg atagagtgct taagagcttc tacgctaagc gtgggcgatg
    11221 aatatgtaaa cggagcattc atataggcgt ttcctgaaaa ctatttcaag cgatagtaaa
    11281 gctcacggta cgacttcgcc gcgacctgcc agctaaaatc cattgccata gcctgacgtt
    11341 gcacaaaccg ccacagagaa ggacgggacc acagtacaaa agcacgtcga atagcccgta
    11401 acagcgacca ggcattacta tcttcaaaga caaacccact ggcgacgcca tctgcaaggt
    11461 tctcaagaga acagtcagaa accgtatcag caagcccacc ggtgcgccgc actaacggca
    11521 gcgtaccgta cttcaatcca taaagttgcg ttaagccgca cggttcaaaa cggctgggca
    11581 ccagaatgac gtccgcaccg cccataatgc gatgcgaaaa tgcttcgtga tagccaatct
    11641 gaacgcccac ctggccgggg tattccgctg ccgccgcaag gaaaccttcc tgcagcaccg
    11701 gatcgcccgc gccgagtagc gccagctgcc cgccctgctc cagaagaccc ggtaaggcct
    11761 ccagcaccag gtcgaggcct ttctggctgg tcagacggct caccactgca aaaagcggca
    11821 ctttatcgtc aaccttaagc cccattgcga tttgtaactg gcgcttattt tccgctttat
    11881 cttccaacgt atcgcgggtg taacgcgagg ccaatagtaa gtccgtctct ggactccaga
    11941 ttttctcgtc cacgccgttc agtacgccgg aaagacgccc ttcacgatga cgctgttgca
    12001 acaaaccttc cataccgtag gcaaactgcg gttcggtgat ctcgcgagcg taggttggac
    12061 tgaccgccgt aatgtgatcg gcatagtaca gaccggcctt caggaaagag atttgtccgt
    12121 tgaattccag cccatgaata ttaaagaatg accatggcaa ttggatgtca ttcatgtgat
    12181 gtgcataaaa catgccttga taggccaggt tgtgcacagt aaacaccgac ttcgccggac
    12241 gcccgcgcgc cgccagatac gcaggcgcaa ggcctgcatg ccagtcgtgc gcatgcacca
    12301 catcaggacg ccagaatggg tcaagcccgc tggccatttc tgccccaacc caccccagca
    12361 gcgcaaaacg caatacgttg tcggtatagg caaataagtt ggtatcgtga tacgggcttc
    12421 ccggacgatc atagagatgc ggcgcgtcaa tcaggtatat gccaaccccg ttgtaatgac
    12481 cgaacaacag cgtgatatgc ccggcgaagg tatcacgacg ggatactacc tgcgcatcgg
    12541 tcacgccacg gcgaatatcg ggaaatgcag gcaacagtac gcgagcgtca acgccgtctg
    12601 cgatttgtgc tgcgggtaat gccccaataa catcagccag accgccggtt ttaagcagcg
    12661 ggaacatctc tgaacataca tgtaaaacct gcattatcgc tcctgtttat gccctaactt
    12721 ccgtagcatt tcgcgcgtta ccagcacgat gccttcttct gaacgataga aacgacgtgc
    12781 atcttcctct gcgttttcac caatcaccat gccttccgga ataacacaag cacgatcgat
    12841 gacgcagcgg cgcagacggc acgagcgacc tacccatact tccggtaaca atacggcgga
    12901 atcaatgttg cagaatgaat tcacgcgaac gcgcgagaac agaacggact gcaccaccac
    12961 cgaaccggag atcacacaac cgccggaaac cagtgagtta agggtcatcc cgtggctacc
    13021 ggagcgatcc tgcacgaatt tcgctggcgg taatgattca ttgtaggtgc gaattggcca
    13081 attgcgatcg tacatatcca gttccggcac cacagaggcc agatcgaggt tcgctttcca
    13141 gtaagcttcc agcgtaccca catcgcgcca gtacggctcg gcatccggat cggattgtac
    13201 gcaagagagc gggaacgggt gcgcataggc cagaccggct tcggtgatct tgggaatcaa
    13261 atctttgcca aagtcgtggc tggagttctc atcgcgatcg tcttcttcca gcagttcata
    13321 cagatagtcg gcgtcaaaga cgtagatacc catactcgcc agagatttgc tcggatcgtt
    13381 cggcattgac ggcgggttag caggtttttc aacgaattcg ataattttat cgttctcatc
    13441 aaccgccata acgccaaatg cggaggcttc ttcaatcggt actggcatac aagcaacggt
    13501 gcaacgtgcg cctttttcga cgtgatcgat aagcatacgc gagtagtctt gcttgtagat
    13561 atggtcgccc gccaggatca ccacgtattc cgctttataa cggcgaataa tatcgaggtt
    13621 ttgggtgacc gcatctgcgg tgccgcgata ccagttttcc cctttcattc tctgctgtgc
    13681 tggcagcaga tcgacaaact cgttcatttc ttcattgaag aatgaccagc cgcgctgaat
    13741 gtgctgcacc agagtgtggg actggtactg ggtgatcacg cccatacgac ggatcccgga
    13801 gttgatgcag ttagacagcg caaagtcgat aatgcggaac ttaccgccga agtgtacggc
    13861 cggttttgct cgcttattgg ttaaatcctt caggcgggta ccacgtcctc ccgccagtat
    13921 cagggcaaca gatttcaatg gcagctggcg cgccaacatt aagtgatcgt tcttctctaa
    13981 actaaccatg actaactcct tttttatcat ctctggaaca cacacaatcc gtgtgcaggt
    14041 ccctgccaga cagccgtaat cactgggtta tcctctccag cgaatggggg aatggcgtgc
    14101 cactccccag caggtaaaac aatctctgtt acctcaagcg tggcgttaat tgcgatcaaa
    14161 aagcgatccg agagcagaat ttgcagctgt ttcggcccgt tttgccactc atccgtgctt
    14221 aaaggttgag catatcgatt tagccaacgg acattgccgt cgccttcttc ccaccagcga
    14281 ttctccacca aagcgggaat acgcttgcgc agatggatta acgcggcggt aaatgcggtt
    14341 aaaccactgc ttgcctgcga ccagtccaac caggttaatt ggttatcctg acagtaggca
    14401 ttgttattgc catgctggct gtgaccatgt tcgtcaccgg ccagtaacat cggcgtaccc
    14461 tgggagagca acaacgttgt taacagggcg tgaatgctgt cgcgccgccg ttcaaccagg
    14521 tcaagggtac cgcctacccc ttctttacca tgattgttac tgtaattgtt gttggtcccg
    14581 tcgcgatttt cttctccgtt tgcttcattg tgtttatggt tgaaacaaac gcagtcgcga
    14641 agcgtaaaac cgtcatgcgc ggtgacgaga ttaatcgcgg cactcggcag acgaccatta
    14701 cgtttaaaaa catcgctgga ggcagcaaaa cgcccggcaa acgcccccag aggcaaatca
    14761 taatgtagcc agaaacgacg ggcagcatcg cggaaatgat cgttccactc ggcaaacagc
    14821 ggcgggaaat ttcccacctg ataaccacca ggagcgatat cccacggttc agcaattaac
    14881 ttcacctgcg agagcaccgg gcagttctgg atagcggtaa acaacggcgc atcctgacgg
    14941 aactctggcg tacggcccat gactgccgcc agatcaaagc ggaaaccatc gacgtggcag
    15001 gtttctaccc aataacgcag gcaggcgctg gcataatcca ccaccgccgg gtgactcaaa
    15061 ttgagcgtgt tgccacaacc ggtccagttg tgataatcgc cgtcttctct tatccaataa
    15121 tagctacggt tgtcgatccc acgcagcgag aataacgggc cgtcgaggtc cagttccgca
    15181 ctatggttga gcacgatatc aagaatgact tcgatacccg ctttatgcag tgctttgatt
    15241 gcatcgcgaa actcatgcag cgccgtttct ggcgagcagg catacgccgg atgcagcgca
    15301 aacatcgcca ccgggttgta accccagtag ttacttagcc ccatgcgttg cagacgtggt
    15361 tcactggcaa actgcgccac tggcagcagt tccagcgcgg taatgcccaa ttgtttcaaa
    15421 tagttgatca tcaccggatg cccgagggct ttataagtgc cacggatctc gaccgggatc
    15481 tccgggtgca ggtacgttaa tcctttaaca tgggcttcat aaatgatggt gctgccccac
    15541 ggcgtgcgcg gcggggcatc atcttcccag tcatagtgat caaccaccac tacgcatttc
    15601 ggcgcaatgg cggcgttgtc gcgatagtca ggttcattat gaccggcgtg cagcagcggg
    15661 ttatctttaa actccccgtc aatttgccgc gcgcaaggat caatcaacaa cttcgccggg
    15721 ttaaagcgat gcccctcggc gggttgccag gggccatgaa cgcgataacc ataacgcaaa
    15781 cccgggcgcg catccggcag ataaccgtgc caaatgtcgc cactgtgccc tggcaagtca
    15841 tagcgatgtt cctggccatt ggcgtcaaag acacacagtt ctacccgctc ggcatgagcg
    15901 gagaaaagtg tgaagttgac gccctgaccg tcgtaatgcg cgccgagggg agcgggtttg
    15961 ccaatggcga gttgtgtcat tctgcctccc gaaccagcca gatagtggcc agcggtggta
    16021 gcgtcaggct tagtgaatgc tgacgaccgt ggctggcaat ctcatcgctg tgtaccgtgc
    16081 cgccattacc tgcattactg ccgtgatagt gcatggaatc ggtattgagg atctcacgcc
    16141 atttgcctgg ctgattaatg ccgaagcgat aatcatgacg cggcaccggc gtaaagttac
    16201 tggcaacgat gatttcgtta ccctctttat cgcgacgcac aaagatcagc accgagcgtt
    16261 ctttgtcatc caccaccagc cattcaaagc cgtacgggtc aaaatccagt tcatgcatcg
    16321 ctttatggtg gcggtaggtg aggttcagat cgcgcaccag acgctggaca ccgtggtgcc
    16381 agttatcgcc gccttccaac agatgccagt cgaggctggc gtcatggttc cactcgcggc
    16441 cctgggcaaa ttcgttcccc atgaacagca gtttcttgcc cggaaatgcc cacatccagc
    16501 catagtaagc gcgcaggtta gcgaatttct gccatgcgtc gcccggcatg cggtcgagaa
    16561 tcgatttttt accgtggacc acttcatcat gcgacaacgg caggacgaag ttttcagtgt
    16621 agttgtagag catcccgaag gtcagtttat cgtgatgata ctgacgataa atcgggtcga
    16681 gcttcatgta gtccagggtg tcatgcatcc agccgaggtt ccacttgtac cagaagccca
    16741 gaccgcccat atcctgcgga cgagaaacgc cagggaaatc ggtagactcc tcagccattg
    16801 tcaccgcacc ggaaacctgc tcaccaagaa tacggttggt attacgcaag aattcaatcg
    16861 cttcaagatt ctcgcgcccg ccaaattcgt tcgggatcca ctccccctct ttacggctgt
    16921 agtcacgata aatcatcgac gccaccgcat cgacgcgcag cgcatcaata ccaaaacgtt
    16981 caatccagta aagcgcgtta ccgacgagga agttactgac ttcacggcga ccatagttgt
    17041 aaatcagcgt gttccagtcc tgatgatagc cttcgcgcgg atcgctgtgt tcatacaagt
    17101 tcgtaccatc aaattcggca agcgcaaagt catcagtcgg gaagtggcct ggcacccagt
    17161 cgagaatcac gttcagacca gctgcgtgtg cagcatcaat gaaataacgg aagtcgtcgc
    17221 gagtaccaaa acggcgggtt ggtgcataca ggccggttgg ctgataaccc caactgccat
    17281 cgaagggatg ctcgttaagg ggcagtagtt cgaggtgggt aaagcccatc catttagcat
    17341 aaggcaccag ttgatcggcc agctcgcggt agctcaacca gaaattgttg tcggtgtggc
    17401 gacgccagga gcctaggtga acttcataaa tagagattgg cgcatcaaac tgattcgctt
    17461 ttttgcgctc ttcagtctgt acaacctttt ccggcagccc gcaaataaga gacgcggttt
    17521 ccgggcgcat ttgcgcttcg aaggcataag ggtcggactt cagacgcaag ttgccattgg
    17581 catcaatcat ctcgtattta tagagctgac cgttatgcgc cccagggata aacagttccc
    17641 agatgccgct ctctttacgc aggcgcatcg ggtgacggcg accgtcccag tagttgaatt
    17701 gcccaaccac cgagacccga cgggcgtttg gagcccagac agagaaacgc gtacctgtga
    17761 cgccatccat agtatctgca tgcgcgccta aagtttcata cgggcgcagg tgagtacctt
    17821 cagataatag ccaggcatcc atttcctgga ttagcggacc aaaacggtaa ggatcatcaa
    17881 tcaggttttg ctgcccatgc cagacaacag ccaactgata gcggaaaaaa ttcttacgtc
    17941 gcggaatgac gccactaaag aatccccgtg agtcgagaca ctccagtttt gcgagtttgc
    18001 gcccggtttt cggttcaatc acccacacat cggtagcgtc gggtaaaagg gcacggactt
    18061 ccagtcccgc ggtggtttta tgcattccca gtacggaaaa aggatccgca aaatggcctg
    18121 caattagcgc gttaatcacg tctctatcga tacgatcgga catgcttgtc ttcctgtttt
    18181 attgtgtcac cccatccaat tggatttttg acttctggtt gtgacatttt tttgacctga
    18241 acggcgcagc actctgtgca tcctctctgc gtcgtcctca cttcaggtaa ggctgtgaat
    18301 actcatgtat tcagccaccc ttaaagaata gccaatgctc tatttaactc ccggtaaatc
    18361 atgaaacatc tgcgcttact cctgtattac gcactaacag gggcggcatc gcgccccaga
    18421 tttaatgaat aaagattacg ccagttgacg aagcatccga cgcagcggct ccgcggcccc
    18481 ccacagcagc tggtcgccca cggtaaaggc tgacaggaac tctggtccca tattcagctt
    18541 acgcaggcgg cctaccggcg tggtcagcgt gccggtaacg gcagctgggg ttagctcacg
    18601 catagtgatt tcccgatcgt tcggaacgac tttcgcccac ggattgtgcg cagccagcag
    18661 ttcttccacg gtcggaatcg acacatcttt tttcaattta atagtgaatg cctggctgtg
    18721 gcagcgcaat gccccgacac gcacacataa accatctacc ggaattacgg aagatgtgtt
    18781 gaggatcttg ttggtttccg cctgcccttt ccactcttcg cggctctgac cgttatcgag
    18841 ctgtttgtcg atccacggaa tcaggctacc cgccagcggc acgccaaagt tatccaccgg
    18901 cagctcaccg ctacgggtta aggttgtgac tttgcgttcg atatcgagaa tagcagagga
    18961 cggggtcgcg agttcatctg ctacatggcc atacagatgg cccatctggg ttaataactc
    19021 acgcatatgc cgcgcaccac cgccggaagc ggcctggtag gttgcaacgg acacccaatc
    19081 aacaagatca ttggcgaata aaccacccaa cgacatcaac atcaggctta cggtacagtt
    19141 accgccaaca aaagtcctga tgccattatt taatccgtcg gtaatgacgt cctgattgac
    19201 ggggtcaaga atgatgatgg cgtcatcttt catgcgcaga gacgatgctg cgtcaatcca
    19261 gtaaccctgc catccgcttt cacgaagctt tggatagatt tcgttggtat aatcgccgcc
    19321 ctgacaggtc acaatgatat cgagggcctt tagcgcctcc agatcaaagg catcctgaag
    19381 tgtgccagtg gttccgccaa aagacggcgc agcctgacca agctgagaag tagaaaagaa
    19441 gacagggcga atggcgtcga agtcgcgctc ttcaaccatg cgttgcatga gaacggagcc
    19501 gaccataccg cgccagccga taaaaccaac atttttcata agcgtttttt cctgcaaaga
    19561 tgtgtgctgt ataaatgtgc cggtctcctc ttggcacatc tttcaccata caaaaagcag
    19621 ccaaagtcgc aagtgaaatt aatcaatgat agcgaagcca tcagtaatgc gacttatcct
    19681 gctttgttag cacgcagaaa gtccgcggca attatcaggg aatttgagtt atgaatgaaa
    19741 tcatttctgc agcagtttta ttgatcctga ttatggatcc gctcggaaac ctacctattt
    19801 tcatgtccgt actgaaacat actgaaccga aaagacggcg ggcaatcatg gtgcgagagt
    19861 tgcttattgc tctcctggtg atgctggtgt tcctgtttgc gggtgagaaa attctggcat
    19921 ttctcagcct gcgagcagaa accgtctcca tttctggcgg catcattctg tttctaattg
    19981 ccattaagat gattttcccc agcgcttcag gaaatagcag cgggcttccg gcaggtgaag
    20041 agccatttat cgtgccgctg gcaattccgc tggtcgccgg gccaactatt ctcgccacgc
    20101 tgatgttgtt gtctcatcag tacccgaatc agatgggaca tctggtgatt gctctgctgc
    20161 tggcctgggg cggcaccttt gtcatcctgc tacagtcttc gctattttta cgtctgctgg
    20221 gcgagaaagg ggtgaacgca cttgaacgcc tgatgggatt gattctggtg atgatggcaa
    20281 cccagatgtt cctcgacggc attcgaatgt ggatgaaggg gtaaggttca aacaggtgct
    20341 gcacacaacc agacgcggca cctgttttat tacctgaact gataatgacc ttcgcccaat
    20401 cggataaccc ttccctcttc ttctgcgcgg ttcagccaca cactaatttg ttcgatggtt
    20461 aaaccagtac aagacgccag cctttcaata cttatcgatt cctttgcaag gatcgccagt
    20521 tccgccacaa agagttgata aaaatcgaca ggcgtatacg cgggcattac tgcgtaattt
    20581 gcgtcatctt catgcagcga aaacagttct ggttgagcgt tacggagtgc tctgtcatac
    20641 atcagcccag gaacgtctgg cagtctgatc gtcacattct cagcctgttc agcactccat
    20701 gacgccccat tttctactaa tcgtgaattg gctgattgca tatcctgatt tggtttaacc
    20761 tgcacaggca gccactgatg ttttaatgcc tccatcgccc cggttatcgt tccaccggtc
    20821 attcccgcac gtacaaccat tgcgctttca gcaaggcaat aaatatattt atttcgcgcc
    20881 atcgcattgc cgacggtgaa acgtacctct gggtaaaacg gcgaaatcaa caccaggttg
    20941 cctgctataa gcccttcacg ccatttcact aacgtactcg tttttaacaa gctatcagct
    21001 aatacgccaa cggcagttcc cccggcctcc agtgccgacg ccattgcaca ttcatcaata
    21061 cctcgcgcac caccagagat aacgcaaatc ccctgttggg ccagtttcgc ggccagttgt
    21121 tgggtatagc gcaaatcgtc agtcggagca tctcgcgagc caacaatcgc cataccttcc
    21181 gcttgcagta atgctttatt cccgcagcca aataaaacgg gaggtgcatc cgttcgcaat
    21241 cggtttttca agcgaacggg ataatcagca tctccgcggg ttaagatcca taaaccggcg
    21301 cgttgccact tatctaccgc caacgccaga ctatgaccac gcgccagcaa gccaagaata
    21361 cgctcctgag aaatacgcgg atcgctccag cctgtaagtt tctcttgcgg ttgcggcacc
    21421 agaagctcgg cgggactgat acgttggtgc ttcagccata atgcaaactt cccccattca
    21481 ctattactaa gaggtttata cttactcgcc gccgcgcgag aaaaatcgct ggttagcagg
    21541 agagttgctt gtgcattggc tgaaagattc atcaattttt taccgaggta gaagcaaggg
    21601 caagaggata aaccgtcggg caacctgcct ggcgtaacag tgctgttaga actgtcagcg
    21661 tccatgcaga atccacgata tcgtcaacca gcaacgccgg accaggcatc aaaggagggg
    21721 taatcacaaa cgccccgtcg agattttgac actggtggaa acggttttgc tgcattttct
    21781 gcggtggatt gtccacgact ttttcaatgg catcaataaa aggtaagcca agtttcgccg
    21841 ccagtcgccg ggcaaaatca ggaaccaggt cgaggtgctt taatgaagga acgcagcata
    21901 cccaggttgg ttcaggatgc ggattccagc gttggcgaac catttccgca catgcctcca
    21961 ccagttcatc actgaatcgc cccgtttttt tctcctgtgc tacctgcttt ccccacccgg
    22021 aatatcccca acgagaaagg attcgccccg tagatccttg taaactcgca ggcaagttcc
    22081 ctttaaatcc gtattgcgta aaagccccag ctgccacctg tttattgaga tttaatgaca
    22141 ggtcagcgtg tttaataaat aagtttgccg catggagtaa gccgctatca atgtcgggcg
    22201 acaacaacgg atgctgaaga caactgctac atttaccaca atgttcggca cctggctcat
    22261 cgagtgcgcg gcgcagaaat tgcattttgc actccttggt cgtgatatag ctttcaacgt
    22321 ctgccagctc actttttctc tgattcatca aatggttaat tcgttcatgg tcaggagaaa
    22381 aactgacaat tgttctgcgc cataatttct cggtatacac cacaggcgat ggattttcag
    22441 ctaccaataa tttcagtgct ttttctattt gcccataacg gagattcgtc cgttgttcaa
    22501 tgcctcgtaa ggtaagaccg tcattcacgc taagtacgtt gagtatttca tgaatttgcg
    22561 cctccgcagg aaaggcgctt tcacggaaga atttatgaat agcacggtct tcactgccgc
    22621 aaagcagtat gccaacagca gaatcgatac cgcgaccggc acgccccacc tgttagtagt
    22681 aaccaactat cgaacctggc atctggtaat gaataacaaa ccccaaatcc ggtttgtcaa
    22741 agcccattcc cagcgccgtt gtcgcaacca gcactttaat tttatttgcc agcaacgcct
    22801 gctccagata ttccctggca gtattagaat cttccgcgcc ttcacaagtc acgccgctgt
    22861 agtaagcaaa tgcactgata ccatttttcc ttaaccactc ggcgacaagt tcggcatcgc
    22921 gagtagtcag ggtataaaca attcccgatt tggaaaactg agggataacc gttgctaacc
    22981 atgccagacg ggatgagtgt tcacctaaga ctaaggcatc aagcgccaaa ctttcgcgag
    23041 ccagcgttcc acgctgaatc acaatgtcac ccaattgctg acggatatcc tcaacgacac
    23101 ggttattcgc tgtcgcggtt gtacccagaa caggggtatt cgcaggtagt tgcgcaaaat
    23161 atctaatata cgtcggtaat ccgggcgaaa atcgtggccc caatcagaga tgcagtgcgc
    23221 ctcgtcgacc acaagcaagc caatatgatc ggctatagga tataaaaccg tttcgataaa
    23281 gtcctggttt gccaaacgtt ccggggaaac cagcaagcaa tcgacctcac cttgcagcaa
    23341 cttatcgcta atgcgctgcc attcttctct gtttgtagaa ttcaacgttt cagccgtaat
    23401 acctaaccgt tcagcggcgg caacctggtt acgcatcagg gcaagcaaag gagagatgat
    23461 aattgtcggg ccagcgccgc gatcgcggaa gattttactg gcgatgaagt acacagcact
    23521 tttcccccag ccagtgcgtt ggacaaccag cagttttcgc cgttggttca ccaccgcgtc
    23581 tatggcttcc cattgccctt ccctgaaagt agcggtggta tttcctaaca tccgctgtaa
    23641 aagcaattca gctgcgtgct tttccatctt tatctcctgc gatctttagg cagtgattag
    23701 aacggattat ttgcaggtaa atttgcgaga gagatcaaaa agatgaagag aggagaaaag
    23761 agaaatgaat tactgcgagc aagctcgcag taatttgtcg cagtaattaa ctcaacagct
    23821 ggaacgcaat catcccaacg atggcaccga cagtgccgag gatggtttcc atcatggtcc
    23881 aggttttcag cgtttcggct tcggtcgcgc cggtaaattt accgaacaac cagaaaccag
    23941 cgtcgttaac gtggctgaca acaatcgaac caccggcgat acaaatcgac agcgccgcca
    24001 tttgcgcacc ggagtagttc agttgttcaa taaccggcat caccagtccc accgccgtta
    24061 aacaggcaac ggttgcagaa ccctgaatga tgcgcactgc agcggccagc acgaagcagg
    24121 tgatggcaat cggcaggccc atgccggtta acgcttcgcc cagtgccgga cctacgccag
    24181 agtcaaccag cacctgtttg aacacgccgc ccgcaccaat caccagcaga atgatccccg
    24241 ccggttgcag cgcgtgaccg caaatctcca tcactttgtc ttttggcatg ccctgacgca
    24301 ttgccaggcc ataaatcgct accagacaag caaccagaat cgcggtaaac agatgaccaa
    24361 taaactcgaa ccattcgtaa gcggtagagc cttccggcac aaaacgcgcg gcaatggttt
    24421 tcagccctac cagcaccagc ggcagcagga tcagcgacag gctgaatccg aaggatggca
    24481 ttttgccttc gccgagatgc ggttcgctga tgtcgtcagg aatatgcagc tcaacgtaac
    24541 ggctgatgaa gttaccccac agcggcccgg caataatcat tcccggaatt gccgcacaca
    24601 ggccaatcag gatcatccag ccaaaatcgg cgttcatctg cgatgccagc agcattggcg
    24661 ctggccctgg caccaggaac gccgccgcgg ccgccacgcc tgcgaataat gggattacca
    24721 gcttcaccag gttcgtaccg gtgtggcgcg ccattgagaa agcaacgcta atcagcagaa
    24781 caatcgccac ttcaaagaac agcggtagcg cacagaccag ccccgcaagg ccgatggcat
    24841 aatgcgcgcg gctgtgaccg aaggatttaa gcattttgac ggcaatctga tcgactgcgc
    24901 cggtttcatg taagatcttg ccaaacatag ctcccagggc gacaaccacc gccaggaagc
    24961 cgagggtgcc tcccatccct ttttccatcg tcgctgcgat tttatcgagc ggcataccgg
    25021 aaaaaaggcc agcccccatg gacaccacca ttaaagccag gaaagcgtgc atacgcgcct
    25081 tcatgactaa aaacagcagc agtaaaacag accctactgc tgttaaaaca agcgttaatg
    25141 tagtcactac ttatttgcct tttttaataa cctcaatggt gcttgccaca acaccttcca
    25201 gcggttgatc gatatccacc accagtacat cggtttcgtc cgcacccggc tcctgcagcg
    25261 tttcaaactg cgtcaccaac atttgggttt taaagaaatg gcctttgcgc gctttcaggc
    25321 ggctttcaat cacatcaaaa tcgcctttca ggtagatgaa agagagattc ggattacctt
    25381 cacgcagcaa gtcacgatag tgttttttca atgcagaaca gacgatcagc gacactttat
    25441 tagtgcgctg catagcaaac gcggcgtcgt tcagcgcctg caaccacggt ttgcgatcgt
    25501 cgtcattcag cggttcgcca gacgccattt tttcgatatt gcgccgtgga tggaggaaat
    25561 cgccatcaag aaacgcggca tgaagttgat gcgccacttc acttgcgacc gcagatttgc
    25621 cgctgcccga tacgcccatc aagacgtaaa tgtggtgatc atggttagtc gtgctcaaag
    25681 tggtgccccc acaatacaag aattaaaaat gttacgggta actgttatcg gtaacattgt
    25741 ccagccggac aatatcagaa gcaatatcca tccgtgcctt aagtgtataa gtgtgagcta
    25801 cttcaaattt gtgggcttaa atagatccgc ccggtgacaa ggtgaaacct aaatctaaca
    25861 ttttcggtgt cacagattcg ccacgaatac gcgccagcag gcgttcagcg ccaatactgc
    25921 ccatccgctc acgcggcgtc agcacgctcg caagtcgtgg ctccatcacc tgaccaatgt
    25981 catgaccgtg gaaaccggca atcgccatat cgtcaggaac ttttaacccc agacgctgac
    26041 attcaaacgc cgcgccgacc gccagatcat cattagtaca gaacacgcca tccagctgcg
    26101 gatattcccg ccgtgcctgg cgaatcagtt caataccgga agagtaagaa gaagattgct
    26161 caaccatcac gctatatggc accaggcctg catccagcat cgcctgttcg tatcccttct
    26221 gtttgatgat agtacgttcg tcgagacgtg cgccgagata ggcaatgtgg cgatgcccgc
    26281 gagcaataat ggctgtggtc atctggcgtg ccgcttcaaa gttatcaaaa ccgacggcga
    26341 tatcgagaca cggcgactgg ctgtccatca actccaccac cgggatcccc gccacttcaa
    26401 tcatctttaa ggtgcgcggc gtgtgggtac gttcggtgag gatcagtccg tcgatattcc
    26461 atgagagcat tgattcgaga cgttcttgct ccatttccgg tttataaccg tagtgggcca
    26521 gcatggtctg ataaccgtgc gcgtcggtga cgctttcgat tccgcgtaat acttccgcga
    26581 aaacctggtt ggtgagagaa ggtaacagga cgccaatcgc ccggctggtg gcgttagaga
    26641 ggatatcggg cgcacgattg ggaatatagc ccagttcatc aagagccgcg gcaatcttgc
    26701 cgcgtagagc gacggaaacc tgctccgggt tgcgtaaaaa acggctgacc gtcattttgg
    26761 tcacgcctac acggtcagcc acatcctgaa gtacgggtct tttctttttc atcgtcctga
    26821 aggtacaaaa gagatagatt ctcttagttt aacacggacg catcagaacc ttcccgggga
    26881 aaacagtgct tgtttacaat tattagatat acactaaatc aattgcatcg cattgtgcta
    26941 acgacgctgc aaaaccccgg aagcgtacat cagcacgcga ccggagcgag cgaggaagcc
    27001 tgcaaaaagg caggcttcaa agatgccgtt gagctttaaa ccggcggcag atcgaacagt
    27061 aacacttcgc tgtcgctatc cgcatggatg gagattgcct gctcatccca gattgccaga
    27121 ccatcgctga tcgaggcttt cacgccgtta atggtgacat tgcctttcac cacctggatc
    27181 cagacgcggc gttcagcggc aatctgatgc accgactgct catctttcag caacgcccag
    27241 cggtacagtt ccatatcctg atgcactttc aatgaaccat cacgcgcatc tggcgaaagc
    27301 accagttgtt tgccctgcac tgcatcgaag cgacgctgtt cataacgcgg cgtaatgccg
    27361 ttttcttccg gcatgatcca gatctgatac aggtgcagac gctcggtgct gcttgggttg
    27421 tactctgagt gacgaatacc cgtacccgca ctcataatct ggaactcgcc cgccggaacc
    27481 tgctctttat tgcccatgct gtcctggtgc tcaacagtac cttccagcac gtaggtcaaa
    27541 atttccatat ctttatgcgg gtgagtgccg aagccctgcc ctgcttcaat cacgtcgtcg
    27601 ttaatcacgc gcagcgcgga gaagcccata aagttcggat cgtaatagtt ggcaaaagag
    27661 aaagtatgcc aggagtccag ccagccatga tttgcatgac cacgttcatt tgctttgcgt
    27721 aagtagatca tttgtattcg ccccctgaat gatttcgatg ggcttagtgt ggacccgatc
    27781 tgcctgggat gatagagggt gaaaattgac ccctctgttc aaaaaaattg aactattcga
    27841 ggggccatca aacttacttc gcgagagtca ctgtggaggg agaggcttgc tcgaatccac
    27901 gttcaagtat ttccagattc gtaagaactt cagattcctt gacgtaattt ggcgcaccgt
    27961 gggtgatggt ttgatacaac gcatcataaa cgcgcccgta atcgcccatc tccggcttca
    28021 tctcttctct gaccgtcacg ccctcgtcat tgacatactc cagcacaccg accgaatcat
    28081 ccgctgcgaa tcccggttcg cccggcataa tattagcctt caggctggtt tcctgctggt
    28141 cgataccata tttaataaac gaacctttct taccgtgaac gataaatttc ggataatcga
    28201 ttttcaccag atggctggtt ttgacgatgg cttttagatc gccataaaac agttgcgctt
    28261 cgaaagtatc gtcaggattg gctttattgc gcaggctgcg gatgtcataa gcgacgtgat
    28321 ccgggcgacc gaacagagaa ataatctggt ccatcgtatg cacaccaagg ccatagaacg
    28381 cgccatcctg cggcagccca ggtttggttt ctgccaccgg gcggtaatag tcaaaatggc
    28441 tttccacttc gacaatctct cccagcttgc cgctttcaat cgcttttttc gccgtcagga
    28501 agcaggaatc aaagcgacga ttctgatatg gcgtgacggt cagccctttg cttttcgcca
    28561 gtgcaaacag ctctttcgcc tgcgcaagtg tcggagtgaa cggtttttcg accagcacat
    28621 ttttcccggc ttccatcgcg cgcttcgcgt actcgaagtg gctgtccgca tgggtgcaga
    28681 caacaaccag cttaacatcg ggatcgttta gcacttcgtt gagatcgctg gtgaaatgga
    28741 tatgagagta aatgggagct tgttcttccg gctttgcatg gcggcgaaaa atatgcgcga
    28801 catgccagct atccttgcgg ttaagtacat acggcagatg gtaacgggtg gtgcttttgc
    28861 cgaagccaat aaaggcgcag ttgatgacca tgatttcgtc ctttttaagg tggttattta
    28921 caccttagcg caaagcggac gtggttccta ctggagtgcg cataacgaac acaagcactt
    28981 cccgaggata aattgagaac gaaagatcaa aaaaaaagcc agcacccggc tggctaagta
    29041 atactggaag caatgtgagc aatgtcgtgc tttcaggttc tccgcgaggg tcttcctgat
    29101 cgcgagacaa taataatcat tctcattcgc acttgtccaa cactttttgc aaaaaaatgc
    29161 atttgactcg cattcgaaag tcaatgatgt tgaaagggac atttacccca aagaggacaa
    29221 aggaatgagt gagatagtga tacgccatgc tgaaacacgg gattacgagg ccatcaggca
    29281 gattcacgcc cagccggagg tgtattacaa cacactacag gtgcctcatc cttccgatca
    29341 tatgtggcag gagcgactcg ccgatcgtcc cggcatcaag caactcgtcg cctgtattga
    29401 tggagacgtc gtgggccatc tcaccattga cgtgcaacag cgcccacgcc gcagtcatgt
    29461 tgccgatttt ggtatctgtg tcgacgcccg ctggaagaat cgcggcgtcg ccagcgccct
    29521 gatgcgagag atgattgaaa tgtgcgacaa ctggttgcgg gtagatcgta ttgagctaac
    29581 ggtgttcgtc gataacgcgc cagcaattaa ggtctataaa aaattcggct ttgagattga
    29641 agggactggc aagaagtacg cattacgtaa tggtgaatat gttgatgcgt attttatggc
    29701 gcgggtgaag taaagaatat tctcaaattg cctgttacca atgtgaataa ttagttttgt
    29761 tcatctaatt tgagtttttc cttatattac caccatcatt ttgaaa
//
LOCUS       DAZKIP010000050        28271 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZKIP010000050 DAZKIP010000000
VERSION     DAZKIP010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.050"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(98..1495)
                     /gene="ccp"
                     /locus_tag="ACHOUK_002452"
     CDS             complement(98..1495)
                     /gene="ccp"
                     /locus_tag="ACHOUK_002452"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFP9897889.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLDVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1900..3549
                     /locus_tag="ACHOUK_002453"
     CDS             1900..3549
                     /locus_tag="ACHOUK_002453"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFP9897890.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(3600..4202)
                     /locus_tag="ACHOUK_002454"
     CDS             complement(3600..4202)
                     /locus_tag="ACHOUK_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9897891.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGIMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            4722..5621
                     /locus_tag="ACHOUK_002455"
     CDS             4722..5621
                     /locus_tag="ACHOUK_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9897892.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLNELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPHLSEFLYQHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQNPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            5670..6683
                     /gene="yhjD"
                     /locus_tag="ACHOUK_002456"
     CDS             5670..6683
                     /gene="yhjD"
                     /locus_tag="ACHOUK_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFP9897893.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            7095..8417
                     /locus_tag="ACHOUK_002457"
     CDS             7095..8417
                     /locus_tag="ACHOUK_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9897894.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLSAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(8599..10659)
                     /locus_tag="ACHOUK_002458"
     CDS             complement(8599..10659)
                     /locus_tag="ACHOUK_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFP9897895.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGIAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(10729..11496)
                     /gene="pdeH"
                     /locus_tag="ACHOUK_002459"
     CDS             complement(10729..11496)
                     /gene="pdeH"
                     /locus_tag="ACHOUK_002459"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFP9897896.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTAVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            11728..12657
                     /gene="kdgK"
                     /locus_tag="ACHOUK_002460"
     CDS             11728..12657
                     /gene="kdgK"
                     /locus_tag="ACHOUK_002460"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFP9897897.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESDQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(12753..14249)
                     /locus_tag="ACHOUK_002461"
     CDS             complement(12753..14249)
                     /locus_tag="ACHOUK_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFP9897898.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(14470..15756)
                     /gene="dctA"
                     /locus_tag="ACHOUK_002462"
     CDS             complement(14470..15756)
                     /gene="dctA"
                     /locus_tag="ACHOUK_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFP9897899.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDALNNRAPDGKTHELSS"
     gene            complement(15939..17927)
                     /gene="hmsP"
                     /locus_tag="ACHOUK_002463"
     CDS             complement(15939..17927)
                     /gene="hmsP"
                     /locus_tag="ACHOUK_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFP9897900.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVHEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSISVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSVLQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(18009..21482)
                     /gene="bcsC"
                     /locus_tag="ACHOUK_002464"
                     /pseudo
     CDS             complement(18009..21482)
                     /gene="bcsC"
                     /locus_tag="ACHOUK_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
     gene            complement(21464..22570)
                     /gene="bcsZ"
                     /locus_tag="ACHOUK_002465"
     CDS             complement(21464..22570)
                     /gene="bcsZ"
                     /locus_tag="ACHOUK_002465"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFP9897901.1"
                     /translation="MNVLRSGLVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(22577..24934)
                     /gene="bcsB"
                     /locus_tag="ACHOUK_002466"
     CDS             complement(22577..24934)
                     /gene="bcsB"
                     /locus_tag="ACHOUK_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFP9897902.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPSPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAVLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(24945..27569)
                     /gene="bcsA"
                     /locus_tag="ACHOUK_002467"
     CDS             complement(24945..27569)
                     /gene="bcsA"
                     /locus_tag="ACHOUK_002467"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HFP9897903.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPRPLDPVRYLIQTCWLLIGASRKETPK
                     PRRRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTF
                     SLILALICVTQPFNPLAQFIFLMLLWGGALIVRRMPGRFSALMLIVLSLTVSCRYIWW
                     RYTSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPS
                     VDIFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIA
                     RTTHEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTVGWFLKEKQLAMMQTPH
                     HFFSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGG
                     IAVETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFR
                     LDNPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIAL
                     FVLPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAK
                     GGLVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLI
                     VLGGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQ
                     ILEGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTW
                     ALWQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPR
                     RPERSETAQPSDQALAQQ"
     gene            complement(27566..28271)
                     /gene="bcsQ"
                     /locus_tag="ACHOUK_002468"
                     /pseudo
     CDS             complement(27566..28271)
                     /gene="bcsQ"
                     /locus_tag="ACHOUK_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
BASE COUNT         6673 a         7828 c         7119 g         6651 t
ORIGIN      
        1 ttttataata taaacattta atatgataat attaaaaaca gactttatta tcgtaataaa
       61 aatcgcccaa acattcctgt tcgggcgatc aaattaatta ttgtttatcc tgcatatacg
      121 gcgtgtaaac cccgttcaga ctgtgcagga aagctacgat atcatccaca tcctcctgcg
      181 gcagctcttt gcctacctga tagcgcaaca tcagtttcac cgccccgtcc agcgtcggca
      241 cgtcaccgcg atggaagtac ggtgccgtta aagcaacgtt acgtaaaccg ggtactttct
      301 gacgcaattt atcacgctct tctttagtca cattcatacg accaatatcc gccgccgtaa
      361 tttccccaaa gttaaagtct tttttcagcc ccaacggttc gaaggaacgt ccgccgagaa
      421 taattccacc atgacaagtt gcacatttat tatctttaaa taattgatag ccttttttct
      481 gttgcgccgt cagcgcattt tcatccccac gcaaccattt atcaaatggg gaatccggcg
      541 taattaatgt tttctcaaat tcagcaatgg catcagtaat attttcgcca ctgaaacctt
      601 gcggatagac gtcgaggaac tgcgctttaa gctgcggatc tttttccagc ttagcaataa
      661 tttcgtccca ggattttgac gccatttcaa tcgggttcaa cggcggtcca ccagcctgat
      721 cctgcaatgt tgccgcacga ccatcccaga actgctcaac gttaaatact gagttaaata
      781 ccgtcggcgc gttaatcggc ccaactgcgc caccaacacc aatcgatgtt tttctgccat
      841 cgacgccccc cgcattcaac gcatggcaat gagcgcatga aatggtgcta tcagccgata
      901 aacggggatc gtgatacagc gcaaagccca acgccacttt ttgcgcatcg gtaggcagtt
      961 tttgcgggat gggctgcacc ggttcattgc gatgctccgg agcagtatca ttgctggcgt
     1021 aatattccgc gcgctgtttt gcaatccagg ccagtatttc cgcccgctct tcatcactca
     1081 ccttacccgc ccagtgtagc gcggtataac gcgttggcgg catagtttca tactgcatca
     1141 cccattcaat cttattcaga tcgctttgcg aaacgggttt atcagccagc agagcagcac
     1201 gcacggcctc gaggttaaaa gatttatatc caagcttaat gtcgtaatcc atcaactgtt
     1261 tcgcgccagg aatataataa taggcgggta attctgccga aggcgtgtgg caatagtcgc
     1321 atcctttttc gcggagaaag cctaaaacct tattattttc actgacagca gatgcctgaa
     1381 catcggcctg tttactgcgt ttattatcgt ggtaccacac ataaccagat aaccctaaat
     1441 agcaaatcgc gacgccagcc aggccgatcg cggtaatacg tgagaccatt ttcattattc
     1501 ttccctcacg gtggtgagtt attgtgaccc agaaaatttt ccagaatgct gcaaacgtct
     1561 ctgcgagaca aagccatcat aaaaaacagg gagatacgct ttttgatagg aatcaatcaa
     1621 ttacatgaat ataaactatc aataagatag cctgtgccta ttacccagat caggaatggc
     1681 atatcatgat tgcggcaaga gcaggattga atgttctgaa aatgaaaaac aacacgaaat
     1741 ctgtattttt ataaattcca ttgatattag tgcgttatat tttttgacag gttaataaac
     1801 gtgatccacc gcacgctttg tcgcccacca ggcggagcga atgactaccc ttaaagaaaa
     1861 gcccgataat tagcgacgaa tttcggaggt tggatcctta tgctcaatca gaaaattcaa
     1921 aaccctaatc cagacgaact gatgatcgag gtcgatctct gctatgaact ggacccgtat
     1981 gaattaaaac tggatgagat gatcgaggca gaaccggaac ccgagatgat tgaagggctg
     2041 cccgcctctg atgcgctgac gcctgccgat cgctatctcg aactgttcga gcatgttcag
     2101 tcggcgaaaa ttttccccga cagtaaaacc tttcccgact gcgcacccaa aatggacccg
     2161 ctggatattt taatccgcta ccgtaaagtg cgccgtcatc gtgattttga cttgcgcaag
     2221 tttgttgaaa atcacttctg gctgccggag gtctactcca gcgagtatgt atcggacccg
     2281 caaaattccc tgaaagagca tatcgaccag ctgtggccgg tgctaacccg cgaaccacag
     2341 gatcacattc cgtggtcttc tctgctggcg ctgccgcagt catatattgt cccgggcggc
     2401 cgttttagcg aaacctacta ttgggattcc tatttcacca tgctggggct ggcggaaagt
     2461 ggtcgggaag atttgctgaa atgcatggcc gataacttcg cctggatgat cgaaaactac
     2521 ggtcacatcc ccaacggcaa ccgcacctat tatttgagcc gctcgcaacc accggttttt
     2581 gcgctgatgg tggagttgtt tgaagaagat ggtgtacgcg gtgcgcgccg ctatctcgac
     2641 cacctgaaaa tggaatatgc cttctggatg gacggtgcag aatcgttgat ccctaatcag
     2701 gcctatcgcc atgttgtgcg gatgccggac ggatcgctgc tcaaccgtta ctgggacgat
     2761 cgcgacacgc cgcgtgacga atcctggctt gaggacgttg aaaccgcgaa acattctggt
     2821 cgcccgccca acgaggtgta ccgcgattta cgcgcggggg cggcctccgg ttgggattac
     2881 tcttcccgtt ggctccgtga tactggtcgt ctggcgagca ttcgtaccac ccagttcatc
     2941 cccatcgatc tgaatgcctt cctgtttaaa ctggagagcg ccatcgccaa catctcggcg
     3001 ctgaaaggtg agaaagagac agaagcactg ttccgccaga aggccagtgc ccgtcgcgat
     3061 gcggtaaacc gttacctctg ggatgatgaa aacggcatct accgcgatta cgactggcga
     3121 cgcgaacaac tggcgctgtt ttccgctgcc gccattgtgc cgctctatgt cggtatggcg
     3181 aaccatgaac aggccgatcg tctggcaaac gccgtgcgca gtcggttact gacacctggc
     3241 gggattctgg caagcgagta cgaaaccggt gaacagtggg ataaacccaa cggctgggca
     3301 ccgttacaat ggatggcaat tcagggattt aaaatgtatg gcgatgacct tctgggcgat
     3361 gaaatcgcgc gcagctggct gaagacggtg aatcagtttt atctggaaca gcacaaactg
     3421 atcgaaaaat accatattgc cgatggtgtt ccccgcgaag gcggcggtgg cgagtatccg
     3481 ttgcaggatg ggtttggctg gactaacggt gtggtacgcc gtttaattgg tttgtacggc
     3541 gaaccataat atttttacag ccagccgcta acttcctgct ggctgtaaaa ttatcctctt
     3601 caggaggaga tatttaacat cattgccgcc tgggtgcgat ttttcacttc cagacggcga
     3661 tacagggatt ccagatgcgc ttttaccgtt ccggtactga tattcagcgc tctgccgatc
     3721 tccttatttg attcgcccgc cgctaacatg gttaaaatct cccgctggcg ggcgcttaac
     3781 gatttgagat ctttaatgtc cttttccggc gtcgtccgcc agtctccagg cagaaacatt
     3841 atccccatcg ccgcactatt taccgccaac gcaaatgtct cgacggttga atcacgaggc
     3901 acaatggcca gcacattaaa atggataact tcctgtaacc accgtttatt gcagtccgtc
     3961 gccgtaatta acaccttaac ctcaggaaat tgcaccacgg ttttttgcag caaccagtag
     4021 caaaactcac catcctgatc gccatcgagc ataactaagg cttcagggta actttccagc
     4081 ttttgccata actcgtctgc ctgactggcc ccctgaatac tcactcctgg aatacgctgc
     4141 tgtaaactga ttttcattcc atgaataaat attgactgcc tgtcaaacat gactatttgc
     4201 ataactgaat ctccacctga atacgttaaa aagacttaag tagtggaagg gtattacccg
     4261 cgagaaaaaa taagaattcg ccatttggcg gtggccattc tacagagatg gcgcgtagaa
     4321 aatagttacc gatataaata gttacagcta aacgcctgaa attacatgtc gagggcacta
     4381 tttaaaacaa ttttgaggat ttccttatat tggtggttag tacgcatgca attaaaaatg
     4441 aaattccgcg accacaagcc aaaataacaa atggcaatga gacaaaaata agcacaaata
     4501 gccaatatgt cctctattca ctttaaaggg aatcgctgaa aaatacgctc tgtttaaggg
     4561 gattcacctt tctcagaaag ctattccgcc cttttcctgc tgagaaatcg ccacattcgt
     4621 catgacaaca ttgtgaaacc cggcattaga tgttagaaaa aataataaac aatgcgatat
     4681 gcgcgttact gtttcgctgt gaacaacaat cggtcaaaga aatggataaa attcacgcaa
     4741 tgcagttgtt catcaaagtc gcggagctgg aaagtttttc ccgcgcagcg gatttctttg
     4801 ctttgccaaa aggaagtgtt tcgcgccaga tacaggcact ggaacatcaa cttggcaccc
     4861 agcttctcca gcgcaccacg cgacgggtca aactcacgcc agaaggcatg acctattatc
     4921 aacgagcaaa agatgtgttg agtaatctca acgaactgga cggtctgttt cagcaggatg
     4981 ccaccagtat cagcggtaaa ttacgcatcg acatcccgcc aggaatcgca aaaagcctgt
     5041 tactgccgca cctgtcggaa tttctctatc agcatccggg aattgagctg gaactgagta
     5101 gccatgaccg tccggtagat attcttcatg atggttttga ttgcgtgata cgcactggcg
     5161 cgttaccgga agatggcgtt atcgcccgtc ccctcggcaa actgaccatg gtcaactgtg
     5221 ccagtccgca ctatctgacg cgctttggtt atccgcaaaa ccccgacgat ctcacttcac
     5281 acgcaatagt gcgttataca ccgcacctgg gtgtacatcc gttaggtttt gaggttgcca
     5341 gcgttaatgg cgtccagtgg tttaagtctg gcggcatgtt gacggtaaac agtagcgaaa
     5401 actatctcgc cgccggtctt gccggtctgg ggattattca gatcccgcgc attgccgtgc
     5461 gcgaagccct gcgtgccggg cggcttattg aagtgttgcc aggctaccgt gccgagccgc
     5521 tctccctttc gctggtttat ccgcagcgtc gggagctttc ccggcgtgta aacctgttta
     5581 tgcagtggct ggctggcgta atgaaagagt acctggactg accgactata ctttttaaga
     5641 atgaccacga cagaaggaca aaagagcgga tgacgcagga aaacgagatc aaacgtccca
     5701 cccaggatct ggagcacgag ccgattaagc agttggataa tagcgagaaa ggcggcaaag
     5761 ttagccaggc gctggaaacc gtcaccacca ctgccgaaaa agtccaacgc caaccggtta
     5821 ttgcgcacct gattcgcgcg acagaacgct ttaacgatcg gctgggtaac cagtttggtg
     5881 cggctatcac ctatttctca tttttgtcga tgatcccgat tttgatggtg tcgtttgccg
     5941 cagggggctt tgtgctggcc tcccatccga tgttgctaca ggatatcttc gacaaaattc
     6001 tgcaaaacat cagcgatccg acgctagccg ctacgttgaa aaacaccatc aacaccgccg
     6061 ttcagcagcg tacgactgta gggcttgtcg gcctggcggt ggcgctttat tccggcatca
     6121 actggatggg taacctgcgt gaagcgattc gtgcccagtc gcgcgatgtc tgggaacgct
     6181 cgccgcaaga tcaggagaag ttctgggtaa aatatctgcg tgattttatt tcgctgattg
     6241 gtttgttgat tgcgctgatt gtgacgcttt cgatcacctc ggttgccggt tcggcgcagc
     6301 aaatgattat tagcgccctg cacctgaaca gcattgagtg gctgaaaccg acgtggcggt
     6361 tgattggcct ggcgatttcc atcttcgcca actatctgct tttcttctgg atcttctggc
     6421 ggttgccgcg ccaccgtccg cgcaaaaaag ccctgattcg cggaacattt ctggctgcta
     6481 ttggttttga agtgattaaa atcgtgatga cctacaccct gccatcgttg atgaaatccc
     6541 cctctggcgc agcatttggt tccgtgctgg ggctgatggc atttttctac ttcttcgccc
     6601 gtttgacgct gttttgcgcg gcgtggattg ccaccgccga atataaagac gacccgcgaa
     6661 tgccggggaa aacgcagcct taaaataggc cggatgcagt tcaaaacgcc ttatccggca
     6721 tacgaattta ttcggcctgg ctccccgtag gccgaataag atgcgccagc atcgcatccg
     6781 gctataatgc gcacataacc tcttgaaact ctttcccaga gcctcttcag ccatctattc
     6841 aggagcaaac aatttcatgc caactcataa ccccagcata taaatccagt tggtaacttt
     6901 tatttaacct gaaaccagtt ttatccacta tttataaaat tatgtgaagc atttcataga
     6961 agaaaaatca ctggcctaaa cattatcccc tttttgcctg gtttttgacc atttccgcga
     7021 tttgttacac attgaaatat cacttttgct gtgcgtaata tggctattcg ttagccaaaa
     7081 aataagaaaa gattatgcaa gcaacagcca caacactcga ccacgagcaa gaatacacgc
     7141 cgatcaactc gcgtaataaa gtccttgtcg cctctctcat tggcacggcc attgagttct
     7201 tcgactttta catttacgcc actgcggccg ttattgtgtt tccgcatatc ttcttcccgc
     7261 agggcgatcc tacggcagca acgctacagt cgctcgccac cttcgccatc gccttcgtcg
     7321 cgcgccccat tggctctgcc gtttttggtc attttggcga tcgcgttggg cgtaaagcga
     7381 cgctggtcgc ctcgttgcta acgatgggga tttcgaccgt ggtgattggt ctgctgccag
     7441 gctatgccac gattggtatt ttcgccccgc tgctgctggc gctggctcga tttggtcagg
     7501 gtctgggctt aggtggtgaa tggggcggcg cggcgctgct ggcgactgaa aacgccccac
     7561 cgcgcaaacg tgcattgtac ggctccttcc cgcagctggg cgcaccgatt ggcttcttct
     7621 ttgccaacgg cactttcttg ctgctttcct ggctactgac cgacgagcag tttatgagct
     7681 ggggctggcg tgtgccattt atcttctcgg cggtgctggt cattatcggc ctgtatgttc
     7741 gcgtgtcgct gcatgagtcg ccggtgtttg agaaagtcgc taaagcgaaa aaacaggtga
     7801 agatcccgct gggtacgctg ctgaccaaac atgttcgcgt aaccgtactg ggtacgttca
     7861 ttatgctggc aacctatacg ctgttttaca tcatgacggt ctactctatg acctttagta
     7921 ccgccgccgc gccagttggg cttggcctgc cgcgtaacga agtgttgtgg atgttgatga
     7981 tggcagttat tggttttggc gtgatggtgc cagtcgctgg attactggct gatgcctttg
     8041 gtcgccgtaa aagcatggta atcatcacca cgctgatcat cctgttcgcg ctgttcgcct
     8101 ttaacccact gctcggttct ggcaacccga ttctggtttt tgccttcctg ctgctggggt
     8161 taagtctgat gggtctgacc ttcgggccaa tgggtgcgct gttaccagag ctgtttccga
     8221 cagaagtgcg ttacaccgga gcatcgttct cttacaacgt agcgtcgatt ctcggggctt
     8281 ccgttgcgcc atatatcgca gcctggttgc agactaacta cgggctaagt gcggtggggt
     8341 tatatctggc ggcgatggct ggcttgacgt taatcgccct gctgctgacc catgagacgc
     8401 gacatcagtc gttgtaataa gagtgaaaag cctgatgcgc tacgcttatc aggcctgaat
     8461 ttccatacaa tatattgaat tcgcaaggat ttgtaggccg gataaggcgt tcacgccgca
     8521 tccggcatga acaaagcaca ctttgccaac aatctaaatc tgtttaattg ccggatgtca
     8581 gacatccggc aattatcttc acttcttcat ctgcgacaaa atcgtccggc actgattagc
     8641 ctccccttcg gaaggtgaga tcagtgccag caacgctgcc gctggcgtaa ccagcgttgc
     8701 cagtgccgca gcaacagcac cacgggcaat caacggcccg gctttcaccc cagcctgagg
     8761 atttttaaac gtcccacgca catacagcgg cgaacgcagt gtgataatcc gaataccttt
     8821 actctccgga tcaatagtca aatccagctg ttccgaagca aaacttgccg tgccggtaac
     8881 gttaatcaac gcgttctcag tatcgaaagc aaaaatctgc gggcgtgcca cgccgttggc
     8941 aatattcaga ttcgccgccg cgcagttcac ccgcacctca tcgtcaccaa atatcgcacc
     9001 gacaatgtag ttgccgacat tcagcccaac aatctccatc aggttgcggc tcaccagccc
     9061 gtcattcatc aacagtttca ggttgccgtt actgttgcct aaaagtgccg ccaccgagtt
     9121 accgctaccg cgcagttccg cgtcaccgtt catttccccc agcgtcttct gcatcagttc
     9181 cacatcgggc atcagttctt tcagtttcag tcgacgagcc tgaatatctg cccgcccctg
     9241 catcggcttt ttatcgcctt ccagatgaat attcgccgca atgctgccac ccgcaatgcc
     9301 aaatttcagc ggttgcaggc gcaagtcagc atttttgagg atgatatgag tagaaagatc
     9361 gctaatcggc aggctactgc catgctcaat gcgccgccct ttgaagcgaa catcggcatc
     9421 cataacgtcc catttatcgg tttcgaagcg gtcataaggc agcactttgc ccgcaggctg
     9481 aacgcttttt tcgcccttct tctgttcaga ccgtttcgac ttttctgccc ctttcccgga
     9541 atcaacgcca atcaacggtc ccaggtccgc cagccgcaat tgccgcgact cgacatcacc
     9601 ttccagtttt ggtcgtggct tgccggtggt gtagaccaga gaaccgtgga tatcgctatc
     9661 gccaattcgc ccattaaagc cgcgataatc aaagaccgac gatttttcag tgtcgatttt
     9721 cgctaccagc cgaccatccg tttcaaacgg cggggtatcg ggcagcagaa cgcccgtcag
     9781 ttcatagaga tcacccagtg aatcgccaga aaatttaagc cgtaaatcga caccgcccat
     9841 cttcattggg tcattcacga cgccatcaaa agcaacacgg gtattacctg aacggaaatc
     9901 agcctgtacc ggaaacggcg tcccttcgcc acgcagcgcc agcataccgc ctatttttcc
     9961 cgtacccgtg agcggttcac cgttataacg tccctgcgcc ttcaggccaa aaacgtaatc
    10021 gcccaccttt tctttatccg ctttaccttt cgatccagta acttcgctga acggcagcgg
    10081 cttgcctaag ggatcaacaa aaatctccag atccgctttg cttactttgt catcaatggc
    10141 gatccgccct tgatcgaaaa gaatattatc cagccgaaac gaccatgccg acggctttgc
    10201 attcgcgtct ttgttatcat cgttggcgag attaaacgtc cagttattgt tcttttcaga
    10261 gaggcgaatc aggcgcgcgt cgggcttttc gagcttgatc cacggcagcc agaccgtttt
    10321 ggtcagcagc gccagcgggg ccagcgttgc ttctacgcgt ggcaaatgca ccatcgtgac
    10381 ttcgggaata tccggtggat tgccaagaat gatgtcttcc gcgtgtacat ggggccacgg
    10441 cacccagctg cgccagccag tttcttgctt ttgccgctcc cacaccacgc ccagatcgcc
    10501 acggatagcg aacggacgat tcaactccgc agagactttc tggttgatgg tcggtttgag
    10561 tcgattccag tcaaaagttg caatcaaaat gatcgccacg acaatcaaca acaagaaagc
    10621 ccctgaaatc gcagcggtta ttttgcctgc cttgctcatc gttcgcttcc tcatattctt
    10681 cctgtgccag tcctaaagat agtccagcca ggcggaaaat gaggcagctt atagcgccag
    10741 aaccgccgta ttcagcgttt ctatcggtgc cgggcgtgaa agaaaccagc cttgtgcggc
    10801 gaatgcgggc gagttctgta catcacgcca ctcttccggc gtttctacgc cctcgacaat
    10861 caccccgcga caatagcgat tcattagatg taaaagctga gaaaagagtg tgcgtccttc
    10921 cggcgactga cgcagcatca caaacagttc tcgcgcgatt ttgatgtagt cataacgcac
    10981 ttcacttagt gcagagaaat ttgccatccc ggtaccaaaa tcatccagcc acagcgggcc
    11041 aaattcacac atcgaggcaa aggttgaatc tttcggcaga cggatatgct ccaccagttc
    11101 gaaacgcagc cagggaagac gctcaatctg gcgcaggatt tttggttgct gacgcagggc
    11161 gatgagcgta ggaccatcaa tattgaccga tgccagcagg ccgtgctcta taaagaagtc
    11221 ggctttttgc gccagcaaat caatctgctc tttcacaacc tccatccgat ggctgacggt
    11281 gatttcagta aaatagcgat ccggcggcag gcgttgcgaa gggttcaagg gatgcgtgac
    11341 cgccgttaat agctccacgg ccattaaccg cccgcatgtt tgatagatcg gctgccaggt
    11401 gtaagcacgc tcacactgca accaaaaacg ccgttcctgc aagctctcga tgcttgcttc
    11461 agggttgctt attcgctgga taacctgcct tatcatcaaa gatgtcctgt gtttaaaagt
    11521 gatgcagccc ggactcgaca aagattatcg gcgcgcaagg gcagaacttt accgcctggc
    11581 cggggtaaat ttccccactc cacaacggaa tctttcagga atacgttctg gctcaaaaaa
    11641 ataatggaac actgttttaa tatggttgac cagcaaacca ccacagcgca aactaacgct
    11701 aattttttac agatcaggtt cacgactatg tccaaaaaga ttgccgtgat tggcgaatgc
    11761 atgattgagc tttccgagaa aggcgcggac gttaagcgcg gtttcggcgg cgataccctg
    11821 aacacttccg tctatatcgc ccgtcaggtc gatcctgcgg cattaaccgt tcattacgta
    11881 acggcgctgg gaacggacag ttttagccag cagatgctgg acgcctggca cggcgagaac
    11941 gttgatactt ccctgaccca acggatggaa aaccgtctgc cgggccttta ctacattgaa
    12001 accgacagca ccggcgaacg tacgttctac tactggcgta acgaagccgc cgccaaattc
    12061 tggctggaga gcgatcaatc cgcggcgatt tgcgaagagc tggcgaattt cgattatctc
    12121 tacctgagcg ggattagcct ggcgatctta agcccgacca gccgcgaaaa gctgctttcc
    12181 ctgctgcgcg aatgccgcgc caacggcgga aaagtgattt tcgacaataa ctatcgtccg
    12241 cgcctgtggg ccagcaaaga agagacacag caggtgtacc aacaaatgct ggaatgcacg
    12301 gatatcgcct tcctgacgct ggacgatgaa gacgcgctgt ggggtcaaca gccggtggaa
    12361 gacgtcattg cgcgcaccca taacgcgggc gtgaaagaag tggtggtgaa acgcggggcg
    12421 gattcttgcc tggtgtccat tgctggcgaa gggttagtgg atgttccggc ggtgaaactg
    12481 ccgaaagaaa aagtgatcga taccaccgca gctggcgact ctttcagtgc cggttatctg
    12541 gcggtacgtt tgacgggcgg cagtgcggaa aacgcagcga aacgcgggca cctgaccgca
    12601 agtaccgtta ttcagtatcg cggcgcgatt atcccgcgtg aggcgatgcc agcgtaaacg
    12661 cagcatatgc aaaaggctgg ataaagagtt acccctctat ccagctcatc cataaatacc
    12721 tgatggttgt gctaactcat caccatcagg cattactgtg caggtggaat atccgtcact
    12781 tcaggatgta cgtcatccgt ggcgacagag gtggtcgcag cggcggtaga tggggccatg
    12841 atttgatccc aggccgcctg caacgctttc atgttaaatt ccggctcgcc tttcggctgc
    12901 agcagtatta acgccatatc attcgacaac tgctgacgca gatcctgatt taacatctcc
    12961 acagtcaggc tgttaaggaa atcctgccgc agtttctgat actgctccgg cgcgatatcg
    13021 acgacctgat tttgcaacga acgcatccgc tggcccatca gaatatcggt atcagcccgt
    13081 gcataggcgg caaacagttt ctgcagctcc agtttctttt gcgccactaa cgcattgaac
    13141 tcttcttccg gcagaccttt atcgcgaacc ttcgccagtt cacgcgccac cagattaagg
    13201 ttgctgttca gcttgtcgtt tggtgattcg atgttgatgg cacactgcgc acgcagatac
    13261 agcacacggc agtcaaatcc aagaccgatg tctttgctgt tactggcgct taacgcttgc
    13321 tgaacatgcc agaacagcgc ctcacgggcc aggtccgcac gccagtagcg cagcagtgcg
    13381 gctgattcac gaatcggctg ccacggcgta tcccacatga tagataaccg gtcctgacgc
    13441 accgcgtcag tcataatact caccgcttcc gcacgcagcg gagaaagcgt cggcaccgga
    13501 gccggtgttt cacgtttgcc tttcagttcg ccaaacgttt tgttgatttg gtcgacgacc
    13561 gagcgcgcat ccacgtttcc caccaccagc agcgtcattg catccggggt gtaccatttc
    13621 tgatagaaat ctttaatttt ttccgcttca acgggttgtt tcagcggatc ggcaggatcg
    13681 tgacctaaca aggttgaccc tttcagacga tagcgccacc agccctcttt agtatcggca
    13741 ggccaggttg ccaccatgtc ctgactttgc agcgcgtggt tgatggtttc tggcgtgata
    13801 gtcagtttgc cagtggcatt tgccagataa gagagcgcct ctttcagcaa atcgttacgg
    13861 ttattgggca aactcagatt aaacagcgtg gtgtcataag agacaattac cggcggcatc
    13921 gggcgtttag ggtcgatccc ctgctgccac aatgaacgcg cctgtgctgc gtcaaggcca
    13981 ccgctttgcg ttagcgcaat acgagggatg gcgtgactgt aaccgctctg ttgtgtactt
    14041 tcggcgagcg aaccggtatt aaccagcagg cgaatttcaa cacgatcgct gggacgctgg
    14101 ggggtggtca gcacttgcca ctgtaaaccg ttggaaagcg tcccctgttg ccatgctgga
    14161 tcaggctgga gcgcatctgc ctgcacatag ccagcagtgg ccatcatcag caaaccgccc
    14221 gctaaaagtc gaatttttgt gccctgcatg tgaacccctg atcaactatc ctggtaataa
    14281 aaaaactgcc cgttgtcggt cagtctaaat gacgttaaaa acacttcgtg ttagaccgca
    14341 agaacatgaa aatgtcacgg aagaagtgaa ataaacctga actcacccag gtggtaagtt
    14401 caggcacagg ggtcaattat gcgcaaacac ccgcactcgg ggaagggagt gcgggcataa
    14461 gtgatgagat taagaggata attcgtgcgt tttgccatcc ggcgcacgat tattcagcgc
    14521 atcgtccagt tttttgtggt ccagttcttt cacccactta gcaacgacaa tggtcgctac
    14581 gccgttaccg accaggttag tcagcgcacg agcttctgac ataaagcggt cgataccgag
    14641 gatcagcgcc agacccgcta ccggcaaatg gcccaccgca gagagcgtcg ccgccagcac
    14701 gataaagcca ctacccgtta cccctgccgc ccctttagaa gaaagcagca acacgattaa
    14761 cagcgtgatt tggtggacga tatccatctg actgttagtg gcctgggcga taaacaccgc
    14821 cgccattgtc aggtatatcg atgtgccatc aaggttaaac gagtagcctg tcgggatgac
    14881 cagccccacc accgatttac ggcagccgag tttctccatc ttgtcgagca tacgcggcag
    14941 cgccgactcg gaagatgaag tccccagtac aatcagcagt tcttcacgga tgtagcggat
    15001 aaatttgaag atactgaaac cagtcgcttt agcgattgaa cccaatacca gcaccacaaa
    15061 caggatacag gtaatgtaga aacagataat cagctgcccc agttgcacca gtgtgccgac
    15121 gccgtattta ccgatggtaa acgccattgc cccgaacgca ccaataggtg ccagacgcat
    15181 gatcatattg atgatgccga agatgacctg cgagaaactt tcgatgacgt taaaaatcag
    15241 ttggcctttg ctgcccagac ggtggagcgc aaaaccaaac agtacggcaa acagcagcac
    15301 ctgcagaatg ttaccgctgg caaatgcgcc aatgacgctc gccgggatga catccataat
    15361 gaaggcgaca atgccctggt ctttcgcctg atcggcgtaa accgctaccg ctttcgcatc
    15421 aagcgttgcc ggatcgacgt tcattccggc accaggctgc acgacgttaa cgatgataag
    15481 accaataatc agcgcgatgg tactgacaat ttcaaagtaa agcagtgcga ctgcgccggt
    15541 acgaccgacc gccttcatgc tttccatgcc cgcaatgccc gttacgacgg tacaaaagat
    15601 gacaggagcg atgatcatct taatgagctt aacgaagccg tcgccaagcg gtttcatttg
    15661 ctcgcctatt tcaggataga aatggccaag gagaatacca atggctatcg ctgtcaggac
    15721 ctgaaagtaa aggcttttaa acagagaggt tttcataggg tgtcctttag taaaaccaca
    15781 ggtcttgtaa ggttatgggg tacctgcggc cttaaaataa cacccagaca acatcacaga
    15841 aatgtacctg gatcataatt gaaacaaaaa ggttaaaaag tttgagctgg ctcgcacaaa
    15901 ccagcacttt ttaaagtttt gtaatcagtt tggggtagct acttttcttc caggtaactc
    15961 tcttcgaaga tttcaatagg gagtggacga gcaaaaagga agccctgggc aataccaacg
    16021 cccgcttttg ccagccagtc gcgttgtgct tcagtctcca cgccttcggc aatcatttgt
    16081 aagttcaggc tctgcgccag catgataatt gcagcaatca tgctgctatc ttccggcaag
    16141 ccttcaacaa acattttgtc gattttcaga acgtcgattg gcaacgattt catatgctgc
    16201 agctgacgca gccctgcgta gcccatgccg aaatcatcca gcgccacccg aactccggca
    16261 ttacgcagcg gacggaggat tgccaccgca gcatgagggt cgtcaatacg tcggctttct
    16321 gtcacttcca gaatcagtgt tcccggctga atgcgatagc gggttaacag ttccagcata
    16381 tccgccacca tattcgggtg catcagttgc agcacagaga ggtttaccga caagggcagc
    16441 ataatgccgc gctcttgcca ggctgcaagc aatcgacagg actcttccag cacccagtga
    16501 ccgacggtaa ccatcagccc acagcactca atgcgatcga ttaagccatc cggcaggtcc
    16561 caactgccat ccggttgctg gatacgcagt aacacttccg cactgaccag tttaccgctg
    16621 gtcatctcga cctgtggctg taaccaaata gcaaactgat gattttccag tgcattaagg
    16681 atatcgctct cttccgtcaa ccgcttctgg gcggcttcca tctgctgcgg atcaaagaac
    16741 tgaatctgat tcttgccttt atggcgagcg gtaaatgccg cagaaatagc gcgactgtaa
    16801 agctgttcgg cggtgagatc gccgtagaac atcgccacgc taatgctaca gtgcggacgg
    16861 agttgaatac gttcaatcgg caggcgctcg ctcatgatag tgagcacttg ctgacccaaa
    16921 gtgattgcgt gccacggttc atgtacaccg ttggcaatga cagcaaagtc ataaccgcta
    16981 atctgcgcga ggatcatacg tggcgacagt accgatttga gtttttccac cagcgtcagc
    17041 agcagaattt ctcgttgcgc ctctttcagc acgcccgcag tatcacgcag agtttcacag
    17101 gtgatgatca tcagcgcggt ggtttgttta cgcgcgacaa cctgctccag catctccatc
    17161 agcaaggctt tgttcggcaa atccgacacc gggaagcgca tcgcattttc gttctgttct
    17221 tcataatggc gctgcagcaa ttgctggttg aggttgtaac tgcgcaccaa cataccgatt
    17281 tcatcgtcct gatgcagacg cggtaatgcc agttggtgac caacaagctc ctgggctggg
    17341 atggcgttaa gttcgcgagc aatattgcgt aacggatgca aaatcaggcg gttaatgcac
    17401 cagctaatcg ccaccgtcaa tattaacgac aaaagtaagt aaatggtcac taacgttgag
    17461 agggtgctca tcacgaactt atacatacgg aaggaatccg cctgtagcac cagataggca
    17521 attggctgcg gatttgccgg acgttcgagc gagtagacgc ccagcgagat ttgaaccggt
    17581 agctcaaaca ggcgagtaac cattaccggc accggacgct ctggaataaa acttttacgc
    17641 agcgcctgga actggttagg cagcactaca tcggcacggc tgaccacgcc tgccggttta
    17701 atgctggcaa gaatagcttc cgcttcggga atatcacctt tcaaaatggc tgaagataaa
    17761 ggttcgcgga cagagcgagc aatgctttcc agttgcgtag ccgtgttata gcgattctgc
    17821 tggaccagat ggaacagcaa aacggtgcaa aaaataaaaa cgaacaccag gacaacggct
    17881 gccaccattg ccatctgctt gattgttaac gagcgactta cgcgcaaatt gtctcccccc
    17941 ggaaatccca gattgccgcc cgagtatacc cgatcgcagc ggcattaaga gaggcgctat
    18001 ctgaaaactt accagtcggc gtaaggtatc agcggctgcg gcggtaaatc catgtcaccc
    18061 tgccatccgg cggcggaata acgtacgtag agcaggaaat ggctgggggc gtaatctttc
    18121 gcctgctgga tatcaattgc cgtgccgaca aaccagttgg aagtaacacg tcgttcaagt
    18181 aatgcccgcg ccgtatagcc gaagccctga ctgctgccgc catcgttgga ttgccgcgca
    18241 gcttcttcct gccagtcggt cgggatcaga ttcatcagcg gataacgcgg catggttttg
    18301 gtgcgtgaat gcgaccacga gccagacgcc cccagctccc acgaccagtt ttccgtgcgc
    18361 tcccgccaca tcaccggtat ggcaaacaac aggtattcct gcgggctgta gtaaccgccc
    18421 taaccgagtg agtagccact caggtctttg tcgtaatgcc agatcatgtt gttcaggccg
    18481 attgtgacgc ggcgattgtt ctggttgatg accttatagt aatagcccgt catccagcgc
    18541 acacgccagt tatcttcgac atttttaccg gttaactggt cgccactaag cgatgcccag
    18601 acgccgtttg cttcaccttt atcgtagctc aggcttagcc ccacgccgtc ggcacgcacg
    18661 ccgccccatt ttttcccggt attgctcggg gagtcttttt gcccaccaaa ggccagcaaa
    18721 gaactggaga tgggacgacg gtgggcgtta acggtgtaac ccagcggccc gatatcatcg
    18781 ctgtaactga tgccgccgac cacatccacc acgttgaagc ccatcggcgt ggtgccgata
    18841 tcccagctcc agacgtcatt tcgccagccg accgccacgc tggcaccgga atccgactgg
    18901 ctgcggttgc cgctacagtc ccgtaatgta caggtgcccc agttgtcatc ccatttgcca
    18961 tcggcattag tggagaaact gccgacgttc atattgacga aatcactgcg aaagaacatc
    19021 cgcccgtcag aatacggcgc atccacctgc aacatggtag tgtgcgcttt cagatcggag
    19081 taaccaccgg tgccgctcga accccaataa tcatgctcga gggtgacgtt aagatcctgc
    19141 tggcgataga ggtccgccgc atcgctgcgc acgccgcgtt tcagccagtc atctttctcg
    19201 tcgttacggg tcagtcgggt aaaggtgtcg ttatcctgcg gacgcgtcgt agtcacaccg
    19261 gatgcgacca tggcgtcttt gtaggtttcc agcgcctgct ttggatcgcc cgcctgcgct
    19321 tcaaacttcg caccgtcgcg cagcaccatc gcgctttcca tcgacggtgg ctgagatttt
    19381 gcctgcggga tcaacttatt aaacgtctgc tgcgccgctg cggtatctcc aagctgcgcc
    19441 tgcgccagcg ccacgcgccg ctgtgtgttc agcgaggcgt tatcagtagc gggcagtttc
    19501 gccagctggc tacgtgccgc cgctgtgtca ccggcagcaa tatccacttc cgtcagacca
    19561 agaatggcat cggcgttagt tggctcccgc gtcaggacat tctgatatgc agcgcgggcg
    19621 gcggtgtaat cacgtcgctg cagcgcccag tcagccagcg tgaggtcaat acgcgtggaa
    19681 ggtggttgct gacgcagcat cgcttccgct tcagcttctt tgccgctttc tcgcaggcga
    19741 ttagcggttt ccagcacctg atcgctttgc agtcgattaa ccagctcctg aatattgctg
    19801 ctccactgcg cacgcggcag gctgttgata tgcgccagcg ccgctctgtc ctggtcatga
    19861 ccagagaggt acagcccgta agcgtaaacc tgctccgggt cgttcggctt ctgctgcgcc
    19921 agattgcgca ttaacgtatc ggcctggctg cgttgtccgg cctgccagag atcctgcgaa
    19981 agtcggtaag taatccatac gctgccgggg tccagcgcca gtcgttgccg ctgaagtgct
    20041 gccgcctgcg cccatttgcc ctggttttcc agtgcctctg cctgctgtgc cagacggtcg
    20101 ttttgcaggc tgcgttcgat atcatcaatg ctacgccgct gactggcaga gagtgaggcg
    20161 ataaacgctt cagctttttc tggcgattgc tggcggtaaa tatttgccag cccgcgcacg
    20221 gcgttagtgt tgccgctgtc catacgcaag gtctgctgat aataacgttc ggcggcggga
    20281 taatcttttc gcgccatcgc cacatcgccc agccccagca ctgcataact gtcggtgtta
    20341 tcgacattac gcgcctgctg gaacaggcgt tctgcccggt caggattatt ggctttcagc
    20401 gcagcatcgc cctgctggat cgccagccag tagcggttta ctttcagcag actgttccat
    20461 ttgtcgttgt tgctgctgtg cggatccagt gcgagggctt tttccagatt cgccactgca
    20521 ttggcgcgat cgcctttctg tgaatatgcc tggcccagcg cccccagagc ttcactgtct
    20581 ttcgggtttg cccgcactgc ctgttgtagt tcgggaatgg ctttacccgc cataccagag
    20641 tccaccgccg ctaaaccttg cgcacgagcg cggaaagcag gatcggccag ctgtttttgc
    20701 tgttctgcca gttgcgattg cgcagccgcc acgctatcgc catcactaaa gatagaaaga
    20761 tattttttca gcgccgacac actggcatca ctgacgggca tgtctttaat ctgcccgtac
    20821 cagattttag aggccccttc gcgcccggcg ttcgatttcg ccatctgttc caggacggca
    20881 aaaccttcgt cacggcgatc gctactaaac agcaatagcg ccagattgtt ttgcaggccc
    20941 gtattgcccg gtgcatccgc attgatgcgt tttaactgat taatcgcttc gccacggcga
    21001 gccggaattt tcgccaccgt actccagtac tcgacagcaa tgtcaccttc cggcggcgca
    21061 ccgttgaaca gtttgttgta actcgccaca gcttcttccg catgaccggt agtcgcctgc
    21121 aatcttgcct gttgcagtgc ctgacgacca tccggcgtgg aaagtagcat cgtagtccgc
    21181 gacgatttat acgcatttga actcggcgct aactgcgaca gccgatcgag ctgtttttgc
    21241 gcgccatcaa tatcgccctg acgtaacaaa gaacggaaac gggcggcaac gacgtccggg
    21301 ttattcggat caataagttc cagccgatac aacgactgct gcaccaggtc ttcacgatgg
    21361 gtcgcttcgc ctaaccgaac ttgctccagc aactgttgct gagcggttgg tgctgcctcg
    21421 accatcggca tgacggccag accgagggaa agcgtgaata tgtttagtgt gaatttgcgc
    21481 attcctggcc ccagtcaggt aataactcac cttttgtcga gaagcggaaa cggtgttgat
    21541 cccagccttg tccaaacagg gtcagcacat agttgtaata ggcatcgctg ccgggaaagt
    21601 tatcggccac gcgctggcgc tgaacggcct gcgcatcacg gttttgtaaa aagggcagca
    21661 tggcggcaga aaaaccgacc ggtcctttac cctgcgcttt ccccgtagcc acatccactt
    21721 tttccggcgg ataaccgttt ttctcagtga atgtcgccat cggtttaaac cggttgagca
    21781 tccgcgcttt ctgcggatcg ctgtcaggca tcatgcctac ccacatgtaa acgcggatag
    21841 cgtcgtagct gctgatcaat gttttttcgg cttttagctg ccagcctttg tctttctcat
    21901 agcgcaccca gtctggcgaa aagcctttcg gggcggtttc cagcaataaa cgttgattgg
    21961 tttcgcgcag cgtagtccac ggcgcgccaa agcgggtgaa atactgcgcc agcgtcggcg
    22021 gcaggtagct ggggttaaaa cgccagctgt tatcctcagc aaaacccact ttgcccggta
    22081 acaacatgga acccagccca ggcaccgtca ccacttcctc ccgcgcaata cgttttagca
    22141 acgcgctgcc gatgtcggta taacgctgct ctttccacaa acgccccgcc tccagcaacg
    22201 accaagccat ccagacatca ccatcggagg ccgaattgct gtccagcact tcccacttac
    22261 tgttctcttt cttgccccac agccaggcgg gcaaacgttc ttttaaagaa ccctgagcga
    22321 gattgttctg cgtccagtcg agaatattat cgaaagctgc acggtcgtta gccgccaggg
    22381 caaagaacat gccgtaactt tgcccttcag aggtggtgat tttgcgcgcg tcgctggggt
    22441 cgatgacgcg cccttcctga ctgatgtaat cctttttaaa ctgctcccag gcaggccagg
    22501 tacaggctgc ctgaacacta aaggcagcca acagcagcat cgtcacgagt ccactacgca
    22561 acacattcat cttcaattac tcgttatccg ggttaagacg acgacggctg ataatacgca
    22621 gcaaacgcca cagtacccat gccagcaata tcacactgat agccgccagc accgccagca
    22681 ccgccagcac cgccagcaga atcggatggt ttgccagcgc ataccacaag cgctcgaacc
    22741 acggcagatg acctacgtaa taaacgtcgc caacacgtag gctgttgata ccggactcgc
    22801 ggatcaccgc gaccgaaccg aacatggtgg cgcgtttgcc gctatcgttc accgcatcgt
    22861 taagcatttc ataaccgcgt gggctatctg ccagcagcgc aatcacgctg cgctggtcgt
    22921 tatacggcga ctggaagcca atcaccgccg ccatcgcacc ggaagaggtc agcgttgact
    22981 gggtttctgc cgcgcgatcg ctctcgtccg gcacaatgcc ggggaacggg gtctggcgca
    23041 tcggtgtttt cacccagctt tcggtcgcct gcaccaatag gtcgatctgc ttatcgtctt
    23101 tcagtttgtc cgggatacca ccgatgatca tgatgtcggc atctttgccc tgaatggtgc
    23161 tgccatcatc ggtcaccgtc agattaatcg ccgggaagcc cgtctgtgcg ccgataaaac
    23221 caacagtatt cagcaacgtt tccatctgtg cttcgttagg cgttttcggc atcacggtga
    23281 tggtttgcga cagatccgcc atccggctga acgggaagcc cgcgttagca aaggcgcgta
    23341 gatccggcat cgggatgaag tggtaatact tcgagaagtc gatggtggag tcgtcaccaa
    23401 tcaccacatg attctgcacc ggctggaagg taatacagtt atccaccgaa ccgccaggca
    23461 tcgggttcat atactcaaag tcgaagcgca gctggttggt cgcgcccagt ttcagcgccg
    23521 gaatagagac atccgttttg ccatccagca aaccttgtaa taccggaatc cgcagcagca
    23581 ggcggttcgc ctcctgtttg ctgctcaggt tgaaggattg caggaactgg ttattcaggc
    23641 tgatatccat ccgcgaactg tctttcaccg gcggcatggt gtagcggtaa ttaatatcca
    23701 tatcaatgcc ggtactgcgc atcaggtaga gatctggcgg caggtttagc gaaacattaa
    23761 tcgctgctgg ctcaagaccg ctggattgta actgttcttc ataggttttc agttcaccaa
    23821 aggtgaccgg acgatcggta cgcacccagt tcggcgcatc gtacggctta cgcggtagca
    23881 gcggtttcac ttcattcact accacgcttt caccacggaa cagaatgtta ccctgagcga
    23941 tacctttcgc tgcctgcaac aggtctttgt cgtcacgacc aaacaccacc agcagtttga
    24001 cgtaaggatt ctgcggatgg ttaatcatct caatcaccgg ggcttttacc gccggatgat
    24061 cgcgcaggaa gtccggccgt ttgtcgttgg ttgcaaagac aatggcatta cgatccggca
    24121 gttggttata gagcaccggg aagttctgcc cacgccagcc agaacgcgaa ccaaaccacg
    24181 aggcgacaat ggcagatgct tgttgcagcc caacatccgg cgcacccgca aagaccatcg
    24241 gcaaggtgtt ggtgcggtta tcacgcgggt caaagaatgg caccgggaag tgtgacaggt
    24301 cattcttcac attcagggtc tgataggtca gatccagtcc actgctccgc ccaacatcca
    24361 gccaaagcgt ggtgctggcc gggttttcgc acacgtcctg ataatggccg acaaactcca
    24421 gccgcacacg gttgaagtcg gtaataaaca gtggattaat gggcatttgc gccagcgttt
    24481 ttttacccaa ctgttctttg gtcactggca gcacgcccat cagctcatca ttgagataaa
    24541 cctttaactg cgactggaca ggcagtaacg atggcgatgg cgtgtattcg aggttgagca
    24601 tcgccttcgt caccacttca tcgctgcgca taccaaactc aatgctgccg ttcgggttaa
    24661 tgccacgtag caccatgctg cccggcgacg gcgcaatttg cgcaaaggtc agcttcacat
    24721 cacgcgaagg accgttctgc gccacgactg gcgcatcagc gccctgcacg cccggcatca
    24781 cttgccccac ctgcggattt tgttccgtct gggcggctac agctggctca gcattgatca
    24841 gtggttgcgt tgctggcgtc gcctgcgtca tgaaagaggg gaacgcactc atccccatag
    24901 ccactgcaca aatccagaat agttttcttt tcatcgcgtt atcatcattg ttgagccaaa
    24961 gcctgatccg atggttgtgc cgtttcgctc cgctccgggc ggcgcggaat aaacgataca
    25021 acccaggaaa ccagagaagt cagcacacgg aatatgccct tcaccgaaga aggcgcaaac
    25081 tccgccagat ggcggtagcc acggaagccg agcttcagaa tatccagcag actttccagc
    25141 ggcttatctt ccgggtagct gtcctgccag agcgcccatg tatccgcacg ggcaaacgta
    25201 cactgcacaa aatcgatatg ttgctgggtg gtgagcggca ttaatttcag cccaacttca
    25261 ttacccatca cgcgcgccac ctgggtcggg aagacgtatt cctgctgacc gcgtttaagc
    25321 aacagattca ctttctgccc ttccagaatc tgcgcctgac cgttgatctt gatccccaaa
    25381 ccaccgtcgg agaaatcctg aacggtacac gagaagaggt gaccatcttc gcgggcaatt
    25441 gccgcgggca tcgtcatctc cacgcggtgc gatcggcgta cctgtttgct ttctaccgat
    25501 accgcaactg cgccgccaag aacaatcagg ttgtagaata cccacaccat actgacgacc
    25561 acggtgagca tctcggttgg cgggccatag aagtagcgcc agatgcctac cgcaacgccc
    25621 accaggttga gcagaacaag gaagatgtag ggccgcgaga tcacccagtc gacgtactct
    25681 tcttccacca gtccaccttt ggcggtgacg ttaaatttgc ctttgtgcgg gttaatcagc
    25741 gccaccagcg tcggtggtgc gatataccac gccagcaccg tttcgtagat ttcactccag
    25801 aaagagtggc gatatttgcc ctggatcttg gagttggtca ggctggcatg gatcatatgc
    25861 ggcagcacga acagggcgat catcaacgct ggcgcataga tgatgtaggc atgaagcagc
    25921 aggaacgcca gcggcgcagt caggaagatc agccgtggaa tgcccgacaa gaagtggaac
    25981 atggcgttga cgtagcacag ccgctgagca aacttcagcc ctttaccggt gagcgggtta
    26041 tcgagacgga agatttgcac catcccgcgc gcccagcgaa tacgctgacc gatatgcgcc
    26101 gacagacttt cggtcgccag ccccgccgcc tgcggaatac gcatatacgc ggaggtatag
    26161 ccacgacggt gcaaacgcag agaagtatgc gcatcttcag tcacagtttc gacagcaatg
    26221 ccgccaattt catccagcgg cttacggcga atcaccgcac aggaaccgca gaagaaagtg
    26281 gcgtcccaca tatcgttgcc atcctgcacc agaccataga acagcgtgcc ttcgttcggt
    26341 gttttacgaa aacgccccag gttgcgttca aacgggtccg gcgagaagaa atggtgtggg
    26401 gtctgcatca tcgccagctg tttctctttc aggaaccagc ccacggtcat ttgcaggaac
    26461 gatcgcgttg gtacgtggtc gcagtcgaaa atcgacacga actcgccttt ggcatatttc
    26521 agcgcattgt tgatgttgcc cgctttcgca tgttcatgag tggtacgggc gatatacttc
    26581 acccccacgt tttgcgcaaa ctggcgaaac tcttccctgc cgccatcatc gaggatccag
    26641 atgttcagct tgtctttcgg ccagtcgata cccagcgagg cgtaaatggt atttttcacc
    26701 acgttgagat cttcgttgta agtcgggaca aagatatcca ccgacggcca cagcgacata
    26761 tctttcggca atggcaccgg ctgacgattc agcggccata ctacctggaa gtagccgagc
    26821 accagcacaa tccacgcgta cgtttcagcg aagagcagaa taagcccgca caccaggctg
    26881 accggatcgt cccagttcag cgtagaggta tatcgccacc agatataacg gcaagaaaca
    26941 gtcagcgaca gcacaatcaa cattagcgct gagaagcgcc ccggcatccg ccgtacgatc
    27001 agcgctcccc cccacagcag catcaggaag ataaactgcg ccagcgggtt aaacggctga
    27061 gtaacgcaga ttaacgccag aatcagcgag aaggtgacga tgataccgag gatcaaccgc
    27121 cgcgccccgg cactcaaatg accgagctct tttttctcat caagatgctg tgttttatgg
    27181 ctaacgcgct caggcagctc gttcatccat tgatggtaac gtccacgaat attctgcaga
    27241 cctgaaaatg cccgcctgcg cggtttcggc gtttctttgc gcgatgcacc gatcagtaac
    27301 cagcatgttt gaatgagata acggaccggg tccagcggac gcggacgcgg acgcgaggcg
    27361 ttgatatgcg gatacaggtt tttatgttct gcgcgaatac gctgccagcg cgggtgctcc
    27421 agtggaataa aaatccaggc caggatcatc cagaaacagc cgagcgtcgc gctgaaagcc
    27481 gacgcaccgt gacgacgata atcgcgataa cgcccgataa gccgcgcgtt gaccggcggg
    27541 ataagcaacc accgggtcag gatactcatg atgcactccc gactggcgtt ttcagcccgg
    27601 aatagttcaa caggcaccag ttcgccagcg tcaatatctc ttcagccgcc agcgcatcac
    27661 tgcgatattc acctactggt tgcttagccg ccaggcattc agccatcgct tcatcacgat
    27721 gaatgagcat cggcagtaat cggcgctggc tttgcaacca aagctggtaa atatcgtcct
    27781 gaacctgact gccaatacgg aagttattaa tcaaaatatg tgcgccatcc ggcagcgctt
    27841 gctgatgcag tcggatatgg cagttggcat ccacattgac gattgccagc gagtgatcgc
    27901 acaaactcag cagctggtgg gttatctgcg aggcatcacg cggtaagtcg attaaaatcc
    27961 actggtaacg cccgctggct tttagttgct gtaagccgga gcaaatatcg ctcagccggg
    28021 tttgccagtg ctgtggattt tcttgttctt caatggataa ctgaccaaaa ggcagcaaat
    28081 cgagctgcga ggtgtagcgc aacccagcgt cgcgccagtc ctagccatcc agcatagctc
    28141 tggcccagcc ctgacggtgg gtaaaatcaa cattaaatga caggcgcaac aagttgtccg
    28201 ggcaggcatc gaccaccagg acattttctc ccaacatttg taatgaccag gctaatgcgg
    28261 cggtgatggt t
//
LOCUS       DAZKIP010000051        28018 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZKIP010000051 DAZKIP010000000
VERSION     DAZKIP010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.051"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(33..314)
                     /locus_tag="ACHOUK_002469"
     CDS             complement(33..314)
                     /locus_tag="ACHOUK_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2574 family protein"
                     /protein_id="HFP9897904.1"
                     /translation="MNKYWLSGAVFLAYGLASPAFSSDTTTLTINGRISSPTCSMDVV
                     NNHLQQRCGQLLQRVETNYRASSTAKGVTTEVVAVGNNDKRKIVLNRYD"
     gene            complement(576..1685)
                     /gene="apbC"
                     /locus_tag="ACHOUK_002470"
     CDS             complement(576..1685)
                     /gene="apbC"
                     /locus_tag="ACHOUK_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFP9897905.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1817..3850
                     /gene="metG"
                     /locus_tag="ACHOUK_002471"
     CDS             1817..3850
                     /gene="metG"
                     /locus_tag="ACHOUK_002471"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFP9897906.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYTRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3991..4719
                     /locus_tag="ACHOUK_002472"
     CDS             3991..4719
                     /locus_tag="ACHOUK_002472"
                     /inference="COORDINATES: protein motif:HMM:NF017243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HFP9897907.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHINWGKVGTNGQSQIKSFAD
                     VAAAEKAKLKLIAEKTKKGYVENASANVHIPPITKATPEVETSPESKNQRPWLADDAV
                     IPVTDDINRFAFPHRSRPREINYLRKDGEIWKRIADNTRAYDPDNNYRSYPENWQQAF
                     AELQRRILDNQQTGSAQSDAALLWSFWNSYSADELVDDLVIRCGLESAVEIVLFALQL
                     KYKPVKGAVTTIIPPNHEAESLPS"
     gene            6676..7800
                     /locus_tag="ACHOUK_002473"
                     /pseudo
     CDS             6676..7800
                     /locus_tag="ACHOUK_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            7813..10798
                     /locus_tag="ACHOUK_002474"
                     /pseudo
     CDS             7813..10798
                     /locus_tag="ACHOUK_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            10808..14437
                     /gene="yehI"
                     /locus_tag="ACHOUK_002475"
     CDS             10808..14437
                     /gene="yehI"
                     /locus_tag="ACHOUK_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HFP9897908.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRRWPGEPVSVITGSLIQ
                     TLGDELLQQAGQKENITWNYDKCSLEWQSAIQQAINLTGEHKPSIPALTMAALICIAQ
                     NDSQQLLDEIVQQEGLEYATDVVISRQCIARRYESDSLVVTLQYQDEDYGYGYGSATY
                     NDFDLRLRKHLSLAEESCWQRCADKLIAALPGIPKIRRPFIALILPEKPEIANELASL
                     ESSRSSLHSKEWLKVVATDNTAVKKLERYWGLDVFSDREASYMSQENRFGYAACASLL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPGYPIIEDKKLPAQQKAREEYWRTLLQTLMASQPQLAAE
                     VMSWLSTQARAVLKSYLSALPKPVIDGTDNSNLPEILVSPPWRSKKKMTAPRLDLARL
                     ELTPQVYWQPGERERLAATESARYFSTESLAQRMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPEAQLAEQALRSGHIEALINIWEND
                     NYSRYRPEKSVWNLYLLAQLPREMALTFWLRINEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTATALIPL
                     VFTKSSDKSEAALLALRLLYEHGHGELLQTVANRWQRTDVWPALEHLLKQGPMEIYPA
                     RIPKAPDFWHPQMWSRPRLITNNQPVTDDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQGKSARA
                     VSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRLV
                     PTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPEA
                     LARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWGV
                     YPANEPRRLLNAFRVAAEGGFCNAQDEPIDLPADALIGIAHPLEMTAEMRSEFAQLFA
                     DYEIMPPFRQLARRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAFV
                     YDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYRDNKSVTFAELDVFNLSEALSAP
                     DVIFH"
     gene            14499..14816
                     /gene="yehK"
                     /locus_tag="ACHOUK_002476"
     CDS             14499..14816
                     /gene="yehK"
                     /locus_tag="ACHOUK_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HFP9897909.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRSTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     EIE"
     gene            16057..17145
                     /gene="yehL"
                     /locus_tag="ACHOUK_002477"
     CDS             16057..17145
                     /gene="yehL"
                     /locus_tag="ACHOUK_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFP9897910.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            17156..19435
                     /gene="yehM"
                     /locus_tag="ACHOUK_002478"
                     /pseudo
     CDS             17156..19435
                     /gene="yehM"
                     /locus_tag="ACHOUK_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
     gene            19428..20564
                     /gene="yehP"
                     /locus_tag="ACHOUK_002479"
     CDS             19428..20564
                     /gene="yehP"
                     /locus_tag="ACHOUK_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFP9897911.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSSIPLARDFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRHSEQWQLV
                     LLVDLSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDMAQALVNVGAQIAAMTPGELATWLAENLQS"
     gene            20561..22564
                     /gene="yehQ"
                     /locus_tag="ACHOUK_002480"
     CDS             20561..22564
                     /gene="yehQ"
                     /locus_tag="ACHOUK_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFP9897912.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCTCGASGMCRHRVMLVLSYQRLCATTRPTEKEE
                     EWQPAIWLEELATLPDATRKRAQVLVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVEAKTQQAEFTHLIWQMRSEHVTSSDDPFASEEG
                     NACRQYVQQLSQALWLGGISQPLIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWRMLSAPLRQPGIVALR
                     EYLRQRPPSCIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAVERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVASLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAIGQAELLANDLTA
                     VGFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPLLQQQG"
     gene            22689..23150
                     /gene="yehR"
                     /locus_tag="ACHOUK_002481"
     CDS             22689..23150
                     /gene="yehR"
                     /locus_tag="ACHOUK_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFP9897913.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(23192..23662)
                     /gene="yehS"
                     /locus_tag="ACHOUK_002482"
     CDS             complement(23192..23662)
                     /gene="yehS"
                     /locus_tag="ACHOUK_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFP9897914.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(23709..24428)
                     /gene="btsR"
                     /locus_tag="ACHOUK_002483"
                     /gene_synonym="yehT"
     CDS             complement(23709..24428)
                     /gene="btsR"
                     /locus_tag="ACHOUK_002483"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFP9897915.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(24425..26110)
                     /gene="btsS"
                     /locus_tag="ACHOUK_002484"
     CDS             complement(24425..26110)
                     /gene="btsS"
                     /locus_tag="ACHOUK_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFP9897916.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYLELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISVRREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            26332..26397
                     /locus_tag="ACHOUK_002485"
                     /pseudo
     CDS             26332..26397
                     /locus_tag="ACHOUK_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            26625..26873
                     /locus_tag="ACHOUK_002486"
     CDS             26625..26873
                     /locus_tag="ACHOUK_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFP9897917.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWKSADEWFVH"
     gene            complement(26989..27141)
                     /locus_tag="ACHOUK_002487"
                     /pseudo
     CDS             complement(26989..27141)
                     /locus_tag="ACHOUK_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885624.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(27375..27965)
                     /gene="espM2"
                     /locus_tag="ACHOUK_002488"
     CDS             complement(27375..27965)
                     /gene="espM2"
                     /locus_tag="ACHOUK_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector guanine nucleotide exchange factor
                     EspM2"
                     /protein_id="HFP9897918.1"
                     /translation="MPMNTTGMSFSSFGISCHRENSFRNSFRGKNDEVVKCSIGERTI
                     SFSVRKFSGNILETVRRQSTKDIDEWIKDERIVYPSRVINQEIDNYCFQKNAKISTEE
                     RQRVFFLVSQENQLTLDVKAAQSSINHVIMGSASFGKKMDALCDGMSRDVKNRTSDTI
                     ANLLADKFYQKHIDSDIDIVKLRNDIPDYLMRAIQG"
BASE COUNT         7227 a         7103 c         6849 g         6839 t
ORIGIN      
        1 tttttaaata tgcttaaaac ggtgttggca aattaatcgt agcgatttaa cacgattttg
       61 cgtttgtcgt tattacccac agctaccact tctgtcgtaa cgccttttgc agtagaagaa
      121 gcacgatagt tggtttcaac tcgttgtaac aactgcccac aacgttgctg aagatgatta
      181 ttaacaacat ccatactgca tgtaggggaa ctaattctgc cattgatggt taatgttgta
      241 gtatcagaag agaacgcggg tgatgctagt ccataagcaa gaaaaacagc tcctgacaac
      301 caatacttat tcattgaacg ttatccctgt agtaaaggtt atgccgggta gattgagtgc
      361 ataacacact atagctgtac atccgctaca cagccacgag ggatgataaa gagacagtgg
      421 ttgcacaatc aatcgccttt gtacagtttc ggactaagat ccactgaata ttcgcagtat
      481 taaataagtg ttcattaatg cggcaaaaac aaatacaccg catcaatata acatctctgc
      541 ggtgtatgaa gaataaccag aatggttaat aggcgttaga ccgcgcggaa ggaaatctcg
      601 cctggaatga cttcaccctg ccagtagagc tgagctgcaa cgcggtcagc cagctggcgg
      661 tagatagcgg taaattcgct ctccggacgg ctgataaccg ttggcgtgcc tttatcgaga
      721 tcctcacgta aggagatgtg taacggcatc tgccccagca gctgtgtatg gtatttttca
      781 gccagtttct ctgcaccacc ggtaccgaaa atcggttcat ggtgaccaca gttactgcaa
      841 atatgcacac tcatgttctc gacaataccg agcaccggaa cctcgacttt ctcgaacatc
      901 acaatgcctt ttttcgcatc gatcaacgcg atgtcctgcg gggtggtgac cacgactgca
      961 ccagttactg gaatgttttg cgccagcgtc aattgaatgt caccggtgcc tggcggcata
     1021 tcaagcacca gatagtcgag atccggccac aaggtttcct gcaacatctg cattaacgcc
     1081 ttgctcgcca tcggtccacg ccacaccatc gcattgtcgt cagtgaccag ataaccaatt
     1141 gaattggttg ccaggccatg agacataatc ggtgccatat gggtaccgtc cggcgaagtt
     1201 ggacgctgat tttctgcgcc cagcatggtg ggaattgatg ggccgtagat atcggcgtcc
     1261 agaatgccga ctttcgcccc ttccgccgcc agtgccagcg ccaggtttac cgccgtggag
     1321 gatttaccca cgccgccttt accggaactg accgcaatga tgttcttcac gccattaata
     1381 cccggctgat tcttcacgcg ttttaaggtg gcgatattgt gcgacagttt ccagtcgata
     1441 gctttcgccc cggtgatacg cagcagttcg gcgctacatt gctcttttaa ctcttcaaac
     1501 gcgctatgcc agacgaatgg cataaccagt tccacatgca acgtgtcgtc catccaggcg
     1561 acatggtgca acgctttcag cgtggtgagg ttatgcttca gggttgggtg ctgaaaattg
     1621 gccagtgtcc cggcgaccat tgcgcgcaag gcttctggtg atttggcctg ggattgttcg
     1681 ttcatcccga ctcctttttt gtatagataa accatcagct gatagtttac ctgaagaata
     1741 tagagaagta cttacttaac attttcccat ttggtactat ctaacccctt ttcactatta
     1801 agaagtaatg cctactatga ctcaagtcgc gaagaaaatt ctggtgacgt gcgcactgcc
     1861 gtacgctaac ggctcaatcc acctcggcca tatgctggag cacatccagg ctgatgtctg
     1921 ggtccgttac cagcgaatgc gcggccacga ggtcaacttc atctgcgccg acgatgccca
     1981 cggtacaccg atcatgctga aagctcagca gcttggtatc accccggagc agatgattgg
     2041 cgaaatgagt caggagcatc agactgattt cgcaggcttt aacatcagct atgacaacta
     2101 tcactcgacg cacagcgaag agaaccgcca gttgtctgag cttatctata ctcgcttgaa
     2161 agaaaacggt tttattaaaa accgcaccat ctctcagctg tacgatccgg aaaaaggcat
     2221 gttcctgccg gaccgttttg tgaaaggcac ctgcccgaaa tgtaaatccc cggatcaata
     2281 cggcgataac tgcgaagtct gcggcgcgac ctacagcccg actgaactga tcgagccgaa
     2341 atcggtggtt tctggcgcta ccccggtaat gcgtgattct gaacacttct tctttgatct
     2401 gccctctttc agcgaaatgt tgcaggcatg gacccgcagc ggtgcgttgc aggagcaggt
     2461 ggcaaataaa atgcaggagt ggtttgaatc cggtctgcaa cagtgggata tctcccgcga
     2521 cgcgccgtac ttcggttttg aaattccgaa cgcgccgggt aaatatttct acgtctggct
     2581 ggatgcgccg attggctata tgggttcttt caagaatctg tgcgacaagc gcggcgacag
     2641 cgtaagcttc gatgaatact ggaagaaaga ctccaccgcc gagctgtacc acttcatcgg
     2701 taaagatatt gtttacttcc acagtctgtt ctggccagca atgctggaag gcagcaactt
     2761 ccgcaagccg accaacctgt ttgttcatgg ctatgtgacg gtgaacggcg cgaagatgtc
     2821 taagtctcgc ggtaccttta ttaaagccag cacctggctg aatcattttg acgccgacag
     2881 cctgcgttac tactacactg cgaaactctc ttcgcgcatt gatgatatcg atctcaacct
     2941 ggaagatttc gttcagcgcg tgaatgccga tatcgttaac aaagtggtta acctggcctc
     3001 tcgtaatgcg ggctttatca acaagcgttt tgacggcgtg ctggcaagcg aactggctga
     3061 cccgcagttg tacaaaacct tcactgatgc cgctgaagtg attggtgaag cgtgggaaag
     3121 ccgcgaattt ggtaaagcag tgcgcgaaat catggcgctg gctgatctgg ctaaccgcta
     3181 tgtcgatgaa caggctccgt gggtggtggc aaaacaggaa ggccgcgatg ctgacctgca
     3241 ggcgatttgc tcaatgggca tcaacctgtt ccgcgtgctg atgacttacc tgaagccggt
     3301 actgccgaaa ctgaccgagc gcgcagaagc atttctcaat acagaactga cctgggatgg
     3361 tatccagcaa ccgctgctgg gccacaaagt gaatccgttc aaggcgctgt ataaccgcat
     3421 cgatatgaag caggttgaag cactggtgga agcgtctaaa gaagaagtga aagcagctgc
     3481 cgcgccagtt actggcccac tggcagatga tccgattcag gaaaccatca cctttgacga
     3541 cttcgccaaa gttgacctgc gcgtggcgct gattgaaaac gcagagtttg ttgaaggttc
     3601 tgacaaactg ctacgcctga cgctggatct cggcggtgaa aaacgcaatg tcttctccgg
     3661 cattcgttct gcttacccag atccgcaggc actgattggt cgtcacacca ttatggtggc
     3721 taacctggca ccacgtaaaa tgcgcttcgg tatctctgaa ggcatggtga tggctgccgg
     3781 tcctggcggg aaagatattt tcctgctaag cccggatgcc ggtgctaaac cgggtcatca
     3841 ggtgaaataa tcccccttca aggcgctgca tcgacagcgc cttttcttta taaattccta
     3901 aagttgtttt cttacgattt tgtctctctc taacccgtat aaatactggt agcatctgca
     3961 ttcaactgga taaaattaca gggatgcaga atgagacact ttatctatca ggacgaaaaa
     4021 tcacataaat tctgggcggt tgagcagcag ggaaacgagc tgcatatcaa ctggggcaaa
     4081 gtcggcacta acgggcaaag ccagataaaa agttttgcgg atgttgcagc agcggaaaaa
     4141 gcgaaactga agcttatcgc ggagaaaacg aaaaaaggct atgtggaaaa tgcttcagca
     4201 aacgtgcata tcccccccat caccaaagcc actcctgaag ttgaaacttc ccctgagagt
     4261 aaaaaccaac gcccgtggct ggctgatgat gcagttattc cggtaactga cgatattaac
     4321 cgatttgctt ttcctcatcg ctctcgcccc agagagatca attatctgcg caaagacggt
     4381 gagatatgga agcgtattgc agataacacc agggcatatg atcccgacaa caattaccgc
     4441 tcttatcctg aaaactggca gcaggctttt gctgagttac aaaggcgaat tctggataat
     4501 caacagacag ggagtgcgca atctgatgca gcattgctct ggagtttttg gaactcttac
     4561 tctgcagatg aactggtcga tgacttagtc attcgctgtg gtctggaaag cgccgttgaa
     4621 atcgtccttt ttgcccttca actcaaatat aaaccagtaa aaggcgcagt aaccaccatc
     4681 attccaccta atcacgaagc agaatcgcta cctagttagc atcaacgtct atgtcatcat
     4741 ctttcactcg cttcagaaga tgagtggcaa cgctgtgtag ataaagtact caccgccatt
     4801 ccgacgctat cccccgcacg gcaacctttt gcggcgttac tgctccctga acgcccggat
     4861 atcgcccatg ctatagatct acgctatgca gaccaaaacg tcccggcgat gacctggtta
     4921 agactggtga taaccgatga tactgctctg agtacagtgg aaaaatacga cttcccaccg
     4981 ttgtatcgtg attttcgtaa ttttcgcgct tatctggcaa tgttgttggc aaataatggg
     5041 gtgcgcggtg taagccggat acttcttgag tttacggaag accattctga caatcccact
     5101 tatctcttcg aacgcatcag cgagactgaa aatcttgtca aatggctatg gaaaacgaat
     5161 cacccggatg cgattcaaat tctgatcctc ggtgtaattg gtaagaaaaa gcacctggaa
     5221 tacttaagca aggccagtca aaagcatccc gctgcggcta ttgcggctta tgctactttg
     5281 ctggcaatac atgaagataa agagtggcgt aaagcgcttg tcaaactgat taccgccacg
     5341 ccagagttag tatgcgacgt cattccctgg gttaatgcta aagcggcagg catcttatct
     5401 gaatgtcgtc cgcagtcagt cgctgaagaa tgtgaatatg ccactgtgga tatgttgccg
     5461 gagctgcttg tttcgccgcc ctggatgacc aaagagaaaa agaaaaatac gcctgtgttt
     5521 gatcttccag tgcttccagt tccttctgtt tctgatgtca ctccagagat taccaaaaaa
     5581 ctgacccgta cttaccttgt cacccacttc cagcaaatag cccaacagca ggcgacaaaa
     5641 caaacattgt ttaccgacct gccgccgatc aaaaaagcaa gctgggagaa acacttaata
     5701 ccgcttacac cggagcaaca aatactgtgg catcttggtt ttgaaaaatg gcgggagagc
     5761 ggagaaaaaa tatacgaaaa aattccggcg ccacaaagcg ccgttgatgc cctgctacgc
     5821 ttcgattttc cagcactcaa tgctgaattt gtgcattatc ataacaatgc atacaaaagc
     5881 tggaatctca ttgccctctg ttacctgcct ggtcaacagg ccatttcttt tcttaaccag
     5941 atagtcaaag aagataacta ctctggtgag ggaaatattc tggcaatttt tggtagcgcc
     6001 gccatacctg catttatggc ctgcctgcaa cgtgatccac gacgtctgtg cttcttcccc
     6061 tttttcctcg gtgtcagtga actggcctta ccgatggcgc aacaattaca gaaaaaaatg
     6121 tcttatgaag atgcgcgaaa ctggctaact gattatccac gccatgccgc cgccgggctg
     6181 cttcctgtgg ccctgggcaa aaaaggaaaa gatcgtgatt gtgcccgcca ggcgcttcgt
     6241 ctgctggtga atttaaacca gcgagaaacg attgaggaaa tcgcgcaagg atataatcaa
     6301 cctgatgttt tagctgcgct ggcaacattg ttcgatagcg acccactgga agaatatccc
     6361 gctaaaatcg ccccactgcc cggcttttat cagttcacct tgtggcgcag accacggctt
     6421 aaaagtaaca acctgcctct gtcagatgac gctatgcgcc acctcggcac tatgctgagc
     6481 tttcctcgcg acattaccgc ctacgctggg ctggatatca ttagagaaat cttcacccgc
     6541 gaatcactgg ctgaatttgg ctgggatctg tatcccgcct ggacagaagc tggcgcaccc
     6601 gcaaaagaaa actgggcatt tacttcgctt gggattttag gcaatgacga caccgcgcgt
     6661 aaattaaccc cgcttatccg cgcctggcct ggggaatccc agcataaacg ggcggtgtcc
     6721 gggctggatg tattagctga tattggcagc gatgttgcgc taatgctgct taatggcatc
     6781 gcgcgaaaaa ttaaattcaa agcattacag gaacatgccc gcgagaaaat caacatagtt
     6841 gctgaaaacc gtgggctgac tatggctgaa ctggaagacc gcctggctcc agatttaggg
     6901 cttgatagca gtggctcgct gatactggat ttcggccccc gcaaattcac cgttggtttt
     6961 gacgaaacct taaaacctgt agtgtgcgat gcaaacggca aagtcctgaa agatttacct
     7021 aagccaaacc agagcgatga aaaaactcag gcaactgacg cggttaatct cttcaaacag
     7081 ttgaaaaaag atgtacgcgc catagccagc cagcagattg atcgtctgga acaggctatg
     7141 tgccagcgca gacgctggac ggcagagcag ttccgcctgt ttctggtgga gcatccgctg
     7201 gtacgtcact taacccgacg gctgttatgg ggggtttata acgatgaaaa cgcccttatc
     7261 acctgttttc gcgtggcaga agacagcact tacagcgatg cgcaggatga gttattcacg
     7321 ctgccagcag gaaacatcgg tattccgcat gtgctggaaa tatcccctga atccgccgct
     7381 gcattcaggc aaatttacgc tgattacgaa ctgcttcccc ctttccaaca gctcgagcga
     7441 ggtagctatc accttgctga taatgaacgt aatactcacg aactgacacg ctggcaagga
     7501 cggctttgcc aggccggacg cattgtcggg ctggaacgca gaggctggca acgtctggaa
     7561 gaaagcggca gcgtttatgc gatgcgcaaa acaacccctc atggcgatct cgaacttgaa
     7621 acggaacctt tttcattaat ttatggtgaa acggggtatg gcgaccagca cccggttgaa
     7681 agcgtaaaaa tcacctcgcc agatgatcgc tacggtaaac aatcctcact cactttctcc
     7741 atgctcgacg acatcaccgc cagcgagctg attaacgaca ttgaatcact gtttgattaa
     7801 aaggatttaa caatgaaaac ctatatttat caggacgaaa aatcacataa attctgggcg
     7861 gtggagcagc agggaaacga gctgcatatt aactggggta aagtcggtac caacgggcaa
     7921 agtcaggtaa aaagctttgc agatactgcg gcggcggaaa aagcagcact gaagctgatt
     7981 gcggagaagg tgaagaaggg gtatgtggag caagtagacg ctaacgctca ctcacatgat
     8041 gcctcagcaa agaaagtcct ggtcacagag gaaaaagcct caataaatcg ccaggcagca
     8101 actaatggtt taccctggct ggcagacaac gatcccatta ttcttccccc gaatattgcc
     8161 cgccatgccc tcagccaccg cctttggccg ggagagccag taaaaaaacc aaccaaaccc
     8221 gagaaacaaa atttaatttc ccgattggca aatcgtactt atcattatta taaaaatctg
     8281 actttcgatt acagctcatg ctctgatgaa tgggctgatg cgattagcca ggcaatcagc
     8341 ttaattaagg cccaaaagac tgagataccc gcgcgtacta tggcggtact tacttttctg
     8401 gaaatatgtt tttgtcaggg ctggaatgat gaaacctcat ccgagttgct ggatgaaatt
     8461 gtaagcacct tcggtatcga atacgcaact gaaactgtca tcttctggtg gcaaattgat
     8521 ttcgattttg actataaaga tgaacatttg actttcttta ttaattatgc agatagcagc
     8581 aaggacagtt atagaaggaa taacgacaaa tttagtttac gtctgcgtaa acatttatca
     8641 ctagcagaag aagaagtatg gcaaaattgc attgcaaaat tactcgttgc gttaccagat
     8701 atatcactct atcgccaacc gttaattgcc attttaatgc cagaaatacc cgaagtggcc
     8761 catgagatcg tacatcgttt acaccaggtc gcagatgttc cacaacttga aatgttgaaa
     8821 ctggtagcga cagatccatt cacgctcgaa atactggaga actatcaata cattgatgta
     8881 tttaacacct atggtgccag ttggtcagct acggtattac gggagcaagg tatagcggca
     8941 ctggctcgcc tgactcccta tgctgaagaa gataattgcg gtgacgtact gaaatgtatt
     9001 aatcatccgt tggccattac gaataaaaag tgccgaaaat tttccttctg caacgatagt
     9061 ggcattagcc gaagcattgg taaaaaaaga tgataaacgc aaacatacat tgctggtttc
     9121 attacttaat gcaaatcctt tatttgtcga aaaattagtc atcggattat cgtcgcaagc
     9181 ggcaaattta ttgcaacaat gtcagcaatt gctgaatagt cgcaatgaag attttgcaaa
     9241 ctcagaaatt ctaccaatgg tactagtctc gccgccctgg gaaactcaaa ataaaaataa
     9301 cgacaacagt cctgtgggta aatacccggc gaaaatcccc cctttaccgt tgttttatca
     9361 acctccatta tggactcgcc cgaaactcaa aaatagcagc aaggtactgc cagatagcgc
     9421 actccagcac ctcggtgaaa tgctccgctt ccctcaggaa gaggccctgt atccgggatt
     9481 attgcaggtg aaagatgcct gtaccgccga ctcactggct gaatttgcct gggatctgtt
     9541 taccgcctgg ctgactgcgg gcgcaccgtc gaaagagagt tgggcgttca ctgcgttagg
     9601 cgttcttggt aacgatgaca ccgcccgcaa actgacgcca ttaatacgcg cctggcctgg
     9661 tgaatcccag cataaacgcg ccaccgttgg gttggatatt ctcgccgcta tcggtagtga
     9721 tatcgccctg atgcagctta acggtattgc ccagaaactg aaattcaaag cattacagga
     9781 gcgggcaaaa gaaaaaattg ccggcattgc cgaaagccgc gaactcacgg tggcagagct
     9841 tgaagatcgg ttagcaccgg atctcggtct ggatgataac ggttcgttgc tgctggattt
     9901 cggctcacgc cagttcaccg tcagctttga tgaaacctta aaaccgtttg tgcgtgatgt
     9961 ttctggcagc cgtctgaaag acctgcccaa accaaacaaa agcgatgatg aatcacaggc
    10021 gaacgatgct gttaaccgct acaaactgct gaaaaaagat gcgcgtaccg tcgccgccca
    10081 gcaggtagca aggttggaat ccgccatgtg cctgcgtcgc cgctggtcgc cagaaaactt
    10141 tcagctcttc ctggttgaac atccactggt acgccactta acccgccgtc tgatttgggg
    10201 cgtttatagc accgaaaacc agctactgac gtgcttccgc gtggcggaag ataacagcta
    10261 cagcaccgct gacgatgatc ttttcaccct gccggaaggc gatatctctg ttggcattcc
    10321 tcacgttctg gaaatatcac cgacggatgc taccgccttt ggtcagcttt ttgccgacta
    10381 tgaactgcta ccaccgttcc gtcagcttga ccgtaacagt tacgccctga cagaagccga
    10441 gcgcaacgcc agcgaactga cccgctgggc aggcagaaaa tgcccgagcg gtcgggtcat
    10501 ggggctggcg aataaaggct ggataaaggg caccccgcag gatggaggct ggatcggctg
    10561 gatgatcaaa cctctgggcc gctggtcgtt aatcatggaa atcgatgaag gttttgcggt
    10621 aggcatgtcg ccagccgaac tgagtgccga gcaaatctta agcaagctgt ggctatggga
    10681 aggcaacgca gaaagctatg gctgggggag taattcaaca caggaagcgc agttctccgt
    10741 actcgatgcc atcaccgcca gcgagctaat taacgatatt gaagccctgt ttgaataagg
    10801 aaagagcatg gacaaggaat taccgtggct ggcggataac gcccaactgg aactgaaata
    10861 taaaaaaggc aaaacgccgc tcagtcatcg tcgctggccg ggcgaaccag tgtccgttat
    10921 cactggaagt ctcatccaga cattgggaga tgaattacta caacaagcag gccagaagga
    10981 aaatatcacc tggaattatg ataaatgctc actggagtgg cagtccgcca tccagcaggc
    11041 aatcaatttg actggcgagc ataaaccgtc aatcccagcc cttaccatgg cggctctgat
    11101 ctgtatcgcg caaaatgaca gccagcagtt acttgatgaa atcgtgcaac aagaggggct
    11161 ggaatatgcg acggatgtag tgatatcgcg tcagtgcatt gcgcggcgtt atgagagtga
    11221 ctcactggtc gtcactttgc agtatcagga cgaggactat ggctacggtt atggctccgc
    11281 gacctataac gatttcgatc tccgtttgcg taagcatctg tcgctggcag aggaaagttg
    11341 ctggcagcgt tgtgccgata aactcattgc cgcactgcca ggaataccca aaattcgccg
    11401 tccttttatt gcgctgatcc tcccggaaaa acctgaaata gccaatgaac tcgccagtct
    11461 tgaaagctcc cggtcaagtc tccattcaaa ggagtggtta aaggtcgttg ctactgataa
    11521 tacggcggta aaaaaactcg aacgatactg gggcctggac gtatttagcg atcgcgaagc
    11581 cagctacatg tcacaagaaa accgcttcgg ctacgcggcc tgcgcctccc ttttgcgcga
    11641 acaaggactg gcagccgttc cgcgcctggc aatgtatgct cataaagaag attgcggcag
    11701 tctgctggta caaattaacc atccgcaagt catccgcacc ttgctgttag ttgctgataa
    11761 aaacaaaccc agcctgcaac gtgtggctaa atacagtaaa aacttccccc atgcgacgct
    11821 cgccgcactg gcagaactgc tggcgttaaa agaaccacca gcccgccctg gttatccaat
    11881 catcgaagac aaaaagctgc ctgcacagca aaaagctcga gaagaatact ggcgtacgct
    11941 gttacaaacg ctgatggcat cgcagccaca actggcagca gaagtgatgt cgtggttaag
    12001 tactcaagcc agggcagtgc tgaagagtta tttatcggca ctgccaaaac cggttattga
    12061 tggcaccgat aacagcaatc tgcctgaaat cctcgtttca ccaccgtggc gtagtaagaa
    12121 aaaaatgaca gctccacgtc ttgatttggc acggctcgaa ttaactccgc aagtttactg
    12181 gcaaccaggc gaacgagaga ggcttgccgc cactgagtct gcccgttatt tcagcacgga
    12241 atctcttgcg caacgcatgg aacaaaaaag tgggcgagtt gtattacagg aactgggttt
    12301 tggggatgat gtatggctgt ttttgaatta tatactccct ggaaaactgg atgctgcacg
    12361 caattcactc attgttcagt ggcattacta ccaggggcgg gttgaagaga tcctgaatgg
    12421 ctggaactcc ccggaagcac aattagcaga acaggcgctc cgcagcggtc acatagaagc
    12481 gttaattaac atatgggaaa atgacaacta ctcacgttat cgtccggaaa agagtgtctg
    12541 gaacctgtat ttattggcac agttgccgcg tgagatggct ttgactttct ggctgcgtat
    12601 caatgagaaa aagcatctgt tcgcgggaga ggactatttt ctcagtatcc tcggattgga
    12661 tgcgctacca ggtctgctgt tggctttttc acatcgtcca aaagaaacat ttccgttaat
    12721 tttaaatttc ggcgcaacag aactggccct gcccgttgcc cgcgtctggc gacgtttcgc
    12781 ggcgcagcgt gatctggctc gccagtggat tttacaatgg ccggaacata cggctactgc
    12841 acttatccct cttgtcttca ccaaatctag cgataaaagc gaagctgcat tacttgccct
    12901 gcgtttactt tacgaacatg ggcatggcga attgctacaa actgtagcca atcgttggca
    12961 gcgtacagat gtatggcctg ccctggagca tttactgaaa cagggtccca tggaaattta
    13021 cccggcacgc attccaaaag cccctgattt ctggcatccg caaatgtggt ccaggccgcg
    13081 ccttatcact aataatcaac ctgttaccga tgacgctctg gaaattatcg gcgaaatgct
    13141 gcgctttacc caggggggac gtttttatag cgggctggaa caactgaaaa cgttctgcca
    13201 gccacaaacg ctggcagctt ttgcctggga tctcttcact gcgtggcaac aagctggtgc
    13261 ccccgcaaaa gacaactggg catttctggc gttaagtctc tttggtgacg aaagcacggc
    13321 acgggatcta acaacacaga tcctcgcctg gccacaaggc aaatctgccc gtgctgtcag
    13381 cggcctgaac atccttaccc tgatgaataa tgatatggcg ctgatacagc tgcatcatat
    13441 atcgcaacgg gctaaatccc gccccttacg tgataacgcg gcggaatttc ttcaggtagt
    13501 cgcagaaaat cgcgggctaa gccaggaaga gctagcggac agattagtcc caaccctggg
    13561 ccttgatgat ccgcaggcgt tgagttttga ttttggtccc cggcagttta ccgttcgctt
    13621 cgatgaaaac ctcaacccgg ttatctttga tcagcaaaac gttcgccaga aaagcgttcc
    13681 ccggttgcgc gccgatgacg atcaactgaa agcgcccgag gcactggccc gactaaaagg
    13741 gctaaaaaaa gatgctactc aggtgagcaa aaacctgctc ccgcgtcttg aagctgccct
    13801 acgtaccacc cgacgctggt cgctggcaga ttttcattct ctgtttgtta atcatccctt
    13861 tacccgtctg gttacccagc gattaatatg gggggtttat ccggcaaatg aaccgcgtcg
    13921 tttactcaac gcctttcgtg tggccgcaga gggggggttc tgcaatgcgc aagatgagcc
    13981 aattgacctg cctgcggacg ctctgattgg cattgcccac ccgttagaaa tgacagcaga
    14041 aatgcgcagt gaatttgcac agctttttgc cgattacgaa attatgccgc cttttcgcca
    14101 gttggcgcgc cgcacggtgc tgctcacacc tgatgagtca accagtaaca gcctgactcg
    14161 ctgggaaggt aaatccgcta ccgttgggca acttatggga atgcgataca aaggctggga
    14221 gtcaggttat gaggacgcat ttgtctatga cctgggtgag taccggctgg tccttaagtt
    14281 ttcacccggt tttaaccact acaatgttga tagcaaagcg ttaatgagct tccgttctct
    14341 tcgagtgtac cgtgacaata aatccgtcac ttttgccgaa cttgatgtgt ttaatttgag
    14401 tgaggcgtta agcgcacctg acgtcatttt ccattaaaaa cacagcgggc agtgatgcaa
    14461 ctgcccgtta tcaacacgac ttaccagcgg ggaaagcgat gatcgtgcaa aaagagctgg
    14521 ttgctattta cgattatgag gtccctgtac ctgaagatcc gttttccttc agacttgaga
    14581 tccataaatg ctctgaatta tttacaggtt ccgtctatcg actggagcga ttccggctac
    14641 gttcaacatt tcatcaacgt gatcgagaag atgctgaccc gctaataaat gatgcgttga
    14701 tttatataag agatgagtgt attgatgagc ggaaattacg aggtgaatca cctgaaactg
    14761 taatagcaat ttttaatcgt gaactacaga atatattcaa ccaagaaata gaataatata
    14821 ctctagataa ttaaagtcgc agcacatgca acttgaagta tgacgagtat agctttctta
    14881 aagggggaaa cggtattccc ccttagatgc gtttcaattt gcccgttact tcaacaaatc
    14941 aatcaaccgt aacagagcca ctaataactc cagtacggca atgagcacgg ttaacttttg
    15001 tgtcagcgtg tccatccgct ctccttatta aggagcgcaa caccagcacc ttacccttac
    15061 actgcctgtc gatactggtt tacgtatgtt ggtacgaaac gttgctctcc ggatcagggc
    15121 accgcctgcg gcaccacccg tccggcagcc aggacttaaa attggtccga tccggcacaa
    15181 cctcccaacg ggcaggatta taaatctcac tcactgaatt gcaatggagt gtggatatgg
    15241 ttataattta ttcgttggta cgaaatgttg cacaatcagc aagttagcgt cactgtagta
    15301 aataaaagaa cctgcctcac cagcaggttt ttttattttc tgcgatctac tttccagata
    15361 tttttttgcc aatagcccac cataaatatt cacatcccgg tcaaatggtg cgttgaatat
    15421 aaaatcgatg aatttataaa taaaaatgag ttatatgatg aattaataaa aacagtaatc
    15481 attttattaa agggggaagc ggttttcccc cttagatgcg tttcaatttg cccgttactt
    15541 caacaaatca atcaaccgta acagagctac taatagctcc agtacagcaa tgagcacggt
    15601 taacttttgt gtcagcgagt ccatccgctc tccttattaa ggagcgcaac accagcacct
    15661 tacccttaca ctgcctgtcg atactggttt acgtatgttg gaccacgatg ttgcactccg
    15721 gatcagggca ccacctgcag caccccccgt ccggtagcca ggacttgaaa taggttcgat
    15781 ccggcgcaac gtcccaacag tcaggattat aaatctcatt atcttaattg caacggggtc
    15841 cagccgtggt tataattcgt tcgttggaac acgatgttgc acaggctgtg gtgttaggcc
    15901 tgaaaatagt aaataaaaga acctgcctca ccagcaggtt tttttattta ctgtgatctg
    15961 cttcccagat atttttcgct caaacaacta atgcgccaaa catttattgc gcgtaaaata
    16021 tcgtttattt cattaataca tttcagggat gaatatatgt caccgcagaa taatcatctg
    16081 cagcgtccgc ctgctgctgt gttatacgcc gatgaactgg caaaattaaa acaaaatgat
    16141 aacgcacctt gcccgcccgg ttggcagtta agtttgcctg cggcccgtgc ttttatcctt
    16201 ggcgatagcg cgcaaaatat tagccgtaaa gtggtgatta gtccctccgc tgtcgaacgt
    16261 atgttagtca ctcttgccac cggacgcggt ttgatgttgg tgggggaacc tggcaccgca
    16321 aaatctctcc tgtctgaatt actggcaacc tctattagcg gcgatgccgg gttaaccatc
    16381 cagggcggcg catctactac cgaagatcaa atcaagtacg gctggaacta cgccctgctt
    16441 atcaatcatg gtccatcaac ggaagcgctg gtccctgccc cgctttacca gggaatgcgc
    16501 gacggcaaaa tcgtccgttt tgaagagata acgcgtacac cgctggaagt gcaagactgt
    16561 ctgttaggta tgctttctga cagggtgatg acggtgccgg aactcactgg tgaagccagt
    16621 cagctctatg cgcgagaagg ctttaatatt attgccactg ccaatacccg cgatcgcggc
    16681 gtcaatgaaa tgagtgctgc gctaaagcgt cgcttcgatt ttgaaactgt ctttccgatt
    16741 atggatttcg cccaggagct ggagcttgtc gccagtgctt cggcgcgttt gctggcccat
    16801 agcggtattc cgcataaagt gcctgacgcg gtactggagt tactggtccg caccttccgc
    16861 gatctgcgtg ccaacggcga aaagaaaacg tcaatggata cactaacagc gattatgtcc
    16921 accgctgaag ccgtgaacgt ggcccacgct gtgggcgtcc gcgcctggtt tttagcgaat
    16981 cgcgcggggg agcctgccga cctggtggac tgtattgccg gaaccatcgt caaagataac
    17041 gaagaagatc gcgctcgtct gcgccgttac ttcgaacagc gtgtcgccac acataaagaa
    17101 gctcactggc aggcttatta tcaagcccgc caccgcctgc cgtgaggaaa gatgcatgag
    17161 cgagccgtta attgtcggca tccggcatca tagtccggcc tgcgcccgac tggtgaaatc
    17221 gttaatcgaa agccagcgac cacgatacgt gttgattgaa ggcccggctg attttaatga
    17281 ccgggtagat gaactgtttt tagcccacca gcttccggtg gctatttaca gttattgcca
    17341 gtatcaggac ggtgctgctc ctgggcgcgg cgcctggacg ccatttgccg aattttcgcc
    17401 ggagtggcag gcgctacaag ccgcacgtca tattcaggca caaacttact ttatcgattt
    17461 gccttgctgg gcgcagagtg aagaagagga cgattcgcct gatacgcaag aggaaagcca
    17521 ggccttgctg ctgcgtgcca cccgcatgga taacagcgat accctgtggg atcacttgtt
    17581 cgaagatgaa agccagcaaa ctgcattacc ctctgcactg gcgcactatt ttgcccaact
    17641 gcggggcgat ttccccggcg atgcactcaa tcgtcagcgc gaagccttta tggctcgctg
    17701 gattgcatgg gcagtgcagc aaaataatgg cgacgtgtta gtcgtctgcg gtggctggca
    17761 cgctccggca ctggcaaaaa tgtggcgcga atgcccgcag gacattaaca agccagaatt
    17821 gccctcgctg gcagatgccg ttacaggttg ttatctcaca ccctacagtg aaaagcgcct
    17881 tgatgtgctg gcaggatacc tttcaggaat gcctgccccg gtctggcaaa gctggtgctg
    17941 gcagtggggc ttacagcagg cgggagaaca actgctaaaa acggttctta cccgtttgcg
    18001 ccaacaccac ttgcctgctt cgaccgcgga tatggctgcc gctcatctgc atgcgatggc
    18061 gctggcacag ttgcgcggat ataaactacc gttacgcact gactggctgg atgccatagc
    18121 aggctcgctg attaaagaag ccctgaacgc gccgttgccg tggagctatc gcggcgttat
    18181 tcatcccgat accgatccga ttctgctaac gttgatagac acattagcgg gtgacggatt
    18241 cggtaaactt gccccttcta cgccacaacc gcctctgcca aaagatgtca cctgcgaact
    18301 ggaacgtacc gcaatctctc tttcggcgga gcttacctta aatcgcttta accccaatgg
    18361 actagcgcaa agtcaggtgt tacatcggct ggcaatactg gagatcccag ggattgtacg
    18421 ccagcaggga agcacactga cacttgcagg caacggtgaa gaacgctgga aattaacccg
    18481 cccgcttagc cagcatgcgg cattgattga ggccgcctgc tttggtgcca cactccagga
    18541 agccgcacgc cataaattag aagccgatat gctggacgcg ggtggaatcg gcagtatcac
    18601 cacatgtctt agccaggcgg cgttagcggg tctggcgtcc ttcagtcaac aattactgga
    18661 gcaactcaca ttattaatcg cctaggaaaa tcaatttgcc gaaatgggcc cggcgctgga
    18721 agtgctatat gccttatggc ggctggatga aattagcggt atgcaaggtg cgcagatatc
    18781 acaaacgacg ttatgcgcgg ctatcgatcg cacgctgtgg ctgtgtgaat ctaacggcag
    18841 accggatgaa aaggagtttc acgctcacct gcatagctgg caagcgcttt gccatattct
    18901 gcgcgatcta catagcggcg ttaatttacc cggcgtttct ctttctgcgg cggtagcctt
    18961 actggagcga cgcagtcagg caatccatgc cccggcgctg gatcgcggcg cggctcttgg
    19021 cgcactgatg cgtctggaac atcccaacgc tagtgccgaa gcggcgctga cgatgctggc
    19081 gcagttatcc ccggcacaat ccggcgaagc gctgcacggt ttgctggcgc tggcccgcca
    19141 tcaactggcc tgtcagccgg catttatcgc cggtttcagc agtcatttaa atcaaccaag
    19201 tgatgccgat tttatcaatg ccctgcccga tttacgcgcg gcgatggcct ggctaccgcc
    19261 acgagaacgc gggacactgg cgcatcaggt gcttgaacat tatcaactgg cgcaacttcc
    19321 cgtttcggcg ctgcaaatgc cgttgcattg cccaccacaa gccattgcac atcatcaaca
    19381 actcgaacag caggcactgg catcgctgca aaactgggga gttttccatg tctgaactga
    19441 acgatcttct gaccacccgt gagctacaac gctggcgatt aattcttggc gaagcggcag
    19501 aaacgacgct ttgtgggctg gatgacaacg cccggcagat agaccacgcg ctggagtggc
    19561 tgtatgggcg cgatcctgaa cggctccagc gtggtgaacg ctccggtgga ttaggtggct
    19621 caaatctcac cacccctgag tggatcaaca gtattcacac gctgtttccg caacaggtga
    19681 ttgagcggct ggaaagcgat gccgtactgc gctacggcat tgaagatgtg gtgacgaatc
    19741 tcgacgtgct ggaacgtatg cagccttctg aaagcctgct acgcgccgtt ttgcacacca
    19801 aacatctgat gaaccccgaa gtactggctg ccgcccgccg gatagtgcgc caggttgttg
    19861 aagaaattat ggctcgactg gcaaaggaag ttcgtcaggc tttttctggt gtccgcgatc
    19921 gccgtcgccg ctcatctatt ccactggcgc gagactttga tttcaaaagt actcttcgcg
    19981 ccaatctgca acactggcac ccgcaacacg gcaagttgta tatcgaatcc ccccgcttta
    20041 acagccgaat taagcgccac agtgaacaat ggcaactggt cttactggtt gatctaagcg
    20101 gatcgatggt cgattcggtg atccactctg cggtaatggc ggcctgtttg tggcagttac
    20161 ccggcattcg tacccatctg gtggcgtttg acaccagtgt cgttgatctc acggcagacg
    20221 ttgccgatcc ggtagagtta ttaatgaaag tacagttggg cggcgggacc aatatcgcca
    20281 gtgccgtgga gtatgggcgg caacttattg aacaaccagc aaaaagcgtc attatcctcg
    20341 tgagtgattt ttatgaaggg ggttcatcat cattgctgac gcatcaggtg aaaaagtgtg
    20401 tccagagcgg catcaaagtg ctggggctgg cagcgctcga cagcaccgca acgccttgct
    20461 atgaccgcga tatggcccag gcgctggtta atgttggcgc acaaatagcc gcaatgacac
    20521 cgggtgaact ggctacctgg cttgcggaga atttgcagtc atgaattcac tacgtccgga
    20581 attattagaa ctcacgccgc aggccctgac ggcgttaagc aatgccggtt ttgttaagcg
    20641 cagccttaag gaactggaaa atggcaacgt cccggaaatc agccatgaga acggcgcttt
    20701 aatcgccacc ttcagtgacg gtgtccgtac ccagctggcg aacggccagg cactgaaaga
    20761 ggctcagtgc acctgcgggg ctagcggcat gtgccgtcat cgcgtgatgc tggtgttaag
    20821 ttatcagcga ctttgtgcca ccacccggcc cacggaaaaa gaagaagagt ggcagcctgc
    20881 aatctggctg gaagaactgg ctaccctgcc cgatgccacc cgcaaacgtg cacaggtgct
    20941 ggtcgctaaa ggcatcacca ttgagttgtt ctgtgcgcct ggcgaaattc cctcagcccg
    21001 cttaccgatg agtgatgtgc gtttttattc ccgcagcagc attcgcttcg cccgctgtga
    21061 ttgtattgaa ggcacacttt gcgaacatgt cgtactggct gtgcaggcct tcgtcgaggc
    21121 caaaactcag caagcggaat ttactcattt aatctggcag atgcgcagcg aacacgtcac
    21181 atcatctgac gacccgtttg ccagcgaaga aggcaacgcg tgtcgtcaat atgttcagca
    21241 attaagccag gcattatggc ttggcggcat cagccagccg cttattcact acgaggccgc
    21301 tttcagccgc gcgcagcaag cggcggaacg ctgcaactgg cgctgggtga gtgaatcgct
    21361 acggcaactg cgcgccagcg ttgatgcctt ccacgcccgc gccagccact atcatgcagg
    21421 agaatgctta cgtcagcttg cggcattaaa cagtcgatta aattgcgcac aagagatggc
    21481 ccggcgcgac agtgttggtg aagttcctcc tgtgccgtgg cgcacggtcg ttggctctgg
    21541 cattgccgga gaagcaaagc ttgatcatct gcggctggtg tctttaggta tgcgttgctg
    21601 gcaggatatt gagcattatg gtttacgcat ctggtttacc gatcccgaca ccggcagtat
    21661 tttgcacctt tcgcgcagtt ggccgcgaag tgaacaggaa aactcaccgg cagctacgcg
    21721 tcggctgttt agttttcagg ctggcgcact ggcgggtggg caaattgttt cacaagcagc
    21781 aaaacgcagt gccgatggcg agctgctgtt agctacccgc aaccgcttaa gcagcgttgt
    21841 gccgctgtcg cctgatgcct ggcgaatgtt gagcgcgccg ttacgccagc cgggcattgt
    21901 ggctttgcgg gaatatttac gccagcgtcc cccctcctgc atacggcctc ttaatcaggt
    21961 cgataactta tttattctgc cggtcgctga gtgtatttcg ctcggttggg atagcagccg
    22021 ccagacgctg gatgcgcagg taatcagcgg cgaaggtgaa gataatctgc tgacgttatc
    22081 actgccagcc tcagccagcg cgccttatgc cgttgaacgc atggcggcgc ttttgcaaca
    22141 aacagacgac cccgtgtgtc tggtttctgg ctttgtcagt tttgttgatg ggcaattgac
    22201 actggaacca caggtaatga tgacaaagac tcgtgcctgg gcgctggacg cagaaactgc
    22261 gcctgtggtg gcatcactac cttctgccag cgttctgcct gtgccgtcta ctgctcatca
    22321 gttgctgatg cgctgccagg cattacttat tcaactgctc cataacggct ggcgctatca
    22381 ggaacagagt gctattggtc aggcagagtt gctggcgaat gacctcaccg cggtgggttt
    22441 ttatcggctg gcacatgtat tgggacaatt tcgtaataca gaaagcgagg cacgggtaga
    22501 agcaatgaat aacggtgttt tgctgtgcga acaattattc cccctgcttc agcaacaagg
    22561 atgaaatagt gctttttact aagagttcta ctccagttcc ggactgctca cgccacggta
    22621 ttaggcatat cctatatagc ccctggtgag agtcaccagt tccttgatta aataaaatgg
    22681 agttttacat gaaggctttc aataagctgt tttcccttgt tgttgcatct gttctggttt
    22741 tttctcttgc tggctgcggt gacaaagaag aatcgaagaa attcagcgcc aatctgaacg
    22801 gcactgaaat tgccattacc tatgtctaca aaggtgacaa ggtgcttaag caatcttctg
    22861 aaaccaaaat tcaatttgcc tccattggtg ctaccaccaa agaagatgct gccaagacac
    22921 ttgagccgtt aagcgccaaa tacaaaaaca tcgcgggtgt tgaagaaaaa ttaacctata
    22981 ccgataccta cgcgcaggaa aacgtgacta tcgatatgga aaaagtggat tttaaagccc
    23041 tgcagggtat ttcaggaatc aacgtttctg ctgaagatgc caaaaaaggt atcactatgg
    23101 cgcaaatgga actggtgatg aaagccgctg gttttaaaga agtgaaataa tctgtcggcg
    23161 gccatgtttg cgcatggccg ccatacccgt cttagctttt cttcacatgc tggcgcgctg
    23221 ccagtccacg cagaaaataa cgtaaaaatt gatcgccgca ttcgcggaag tttttatgat
    23281 ccggtgcacg catcatcgcc gtaatttccg gcatcgaaac gcggaactgc tgttcggtga
    23341 ggatcgccag aatgtcatcg gttttcagcg aaaacgcgat gcgtaatttt ttcagcacga
    23401 tgttgttatt aatgcgacgt tccggctcca gtgccggagc agactcatcc ttgccgcgtt
    23461 tttcataaat caggccattg aggaatgacg acaaaacaat gtccggacaa cgctgaaaac
    23521 cctcttcgtc ttctttacgt agccagacgg cgatctgttc cgcggtggct tcgacattac
    23581 ccagcgccag tatacgcacc aggtcattat tattggcttt caaaatgtag cgcacgctgc
    23641 gcagaatatc gttacttagc atgaggcctt caggtgttga tgaggcaaaa agccatttta
    23701 gcagtctttt acaggccaat cgcctctttt aagcttttca gataacggcg gctgaccggc
    23761 acggttaagc cattacgcaa aatcaactcg gcctgaccgt tatcttccag acgaatctcc
    23821 tgtaaatgcg cgaggttaac cagatactga cgatggcagc gcagtagtgg tgtacgactt
    23881 tccagggtac gtaatgtcaa ttcggtaaag ccctctttcc cttcgtggct ggtaacgtag
    23941 acaccgctca tccgactgct gacaaatgcc acatctttca tttgcagcaa ataaatccga
    24001 ctatgccccg tacaagggat aaatttcagc gcctgttgat tttccggtaa cagcgaaaca
    24061 tcctgcttgc tgcgctcctg acgcaatcgc gccagcgttt tctccagtcg cgcttcatca
    24121 attggcttca gcagatagtc aaaggcatgt tcttcaaagg ctttaatagc gtattcgtca
    24181 aacgcggtga gaaaaacaat atacggacga tgttccgggt cgagcatccc caccatttcc
    24241 agaccactga tgcgcggcat ctggatatcg agaaacagca catccgggcg cagtttatgc
    24301 accgcgccga tcccttccac ggcgtttgaa cactcaccaa cgatttcaat atcgctctgc
    24361 tcctgcaaaa atacgcgcag gttctcccgt gctaacggtt catcatcgac aattaagact
    24421 ttaatcatgc ctcgtccctc catggtagtc gtaacgttat tcgggtgtaa ctatcaggct
    24481 cacaggcgac gcttatccca tagtcatcgc caaaccgttc acgtaaacgc ttatccacca
    24541 gattcatccc cagcccactg gcattggtta ccggttgata caaaccggca ttgtcttcga
    24601 tctccagcat caaatgttgc ccctcacgtc ggacgctgat tgccactcgc cctgtatcca
    24661 gcagttgtga tgtcccatgt ttaatggcgt tttccactat cggttgcagg gtaaacgcgg
    24721 gcaattgctg ctgggataat tcttgcggaa tagcaatgtt gacctgcaac cgcgactgga
    24781 agcgcgcctt ttcaatttgc agataagcat tcacatgttc aatttcgtcg gcgagagtaa
    24841 caaactccga aggccgcttt aagtttttgc ggaaaaaagt ggaaagatac tgcaccagct
    24901 ggctggcctg ttcgctgtcg cgacggatca ccgctttaat ggtgttaagc gcattaaaca
    24961 aaaaatgggg gttcacctgg gcgtgaagca gtttgatctc tgactgggtg agcatcgctt
    25021 tttgccgctc atattgcccg gcgaggatct gcgccgaaag cagttgcgca atcccctcgc
    25081 ccagcgtgcg gttgattgaa ctaaataaac ggtttttggc ttcatacaat ttgatggtgc
    25141 ccatcacccg ctgattttca ccacgcaacg gaattaccag cgtcgacccc agtttgcatt
    25201 gcggatgcaa agagcaacga taaggtactt cgttgccatc agcgtagacc acttcaccgg
    25261 tttcaatcgc ttttaaagta taagttgaag aaatcggttt gccgggtaaa tggtggtcgt
    25321 caccaattcc ggtaaaggcc agcaatttct ctcgatcggt aatcgcgact gcaccaatat
    25381 ccagctccag atacagcacc tgagccactt tcatgctgtt cacttcgtta aacccctggc
    25441 gcaaaatgcc ttccgtcgag gctgccactt tcagcgcagt ggcagaaaaa gccgaagtgt
    25501 atttttcaaa catcgcgcgt ttatcgagca atatacgcat aaacagcgcc gcgccgacgg
    25561 tattggtgac catcattggc gcagcaatat tactcaccag acgcaccgca tcttcataag
    25621 gtcgggcgat cgccaggatg atcagcattt gcaccatttc agcgacgaac gtgacggcac
    25681 cagcggtaat ggggttaaag actttatcag tgcgcccgcg acggatcagg atgctgtgta
    25741 ccaggccgcc gagcaatcct tcaacgatgg tcgagatcat gcaacttagc gcggtcatgc
    25801 cccccatcga atatcgatgt aacccgccgg tcaggccaac cagcccaccg acgaccggac
    25861 cgccgagtaa gccgcccatt accgcgccta tcgcacgggt attggcaata gaatcgtcaa
    25921 tgtgcaaccc aaaccaggtg cccatgatgc agaagatgga aaagacgatg tagcagagaa
    25981 atttatgcgg cagacgaacc gtgacctgca ttaacggtat gaataatggc gttttactca
    26041 ttaaccacgc aatgactaaa aaaacgcaca tctgctgaag cagcagcaac accagattaa
    26101 aatcgtacat acccgcaaac cacacatccc tttaaaacgc gtaacataca ttgcctgcgt
    26161 ttaactttct ttgaactctt gcagaaaaat gagaattcgt gagtacgatc actcaaaatc
    26221 gcctggcaaa aataaaatca ccctataaat gcacaaaaaa cgggcaaaac tacctggttc
    26281 gcaaaactgc gtctaaagtt aaaccgggac ctcgcgagca agggtgagac gatggcgctt
    26341 tacacaattg gtgaagtggc gttgctttgt gatattaacc ctgtcacgtt acgcgcgtga
    26401 cgcaacattt ttaaaaatcg acgaagatcg tccaggagcg ccgtttttca agggttggat
    26461 agatgattcg tttaccatgt cgatgaactt cgacgaattt cgccgataaa aagtgtccca
    26521 tacatgcccc attgatgccc cataaacaat atcaggaatt gacactggtt atccatacag
    26581 ataaaaataa cacctgtaca taactacagt tacaggaggg tgttatgcgt gttgagatct
    26641 gtatcgctaa agaaaaaatc actaaaatgc caaacggtgc tgtggatgcg ttaaaggaag
    26701 aattaacccg acgcatcagt aaacgttatg acgatgtaga ggtgatcgta aaagccacca
    26761 gcaacgatgg cctttctgtt acgcgcaccg ccgataaaga ttcagctaaa acttttgttc
    26821 aggagactct gaaagatacc tggaagtctg ctgacgagtg gtttgttcac taattaacac
    26881 gtaaaatcgg taacggctgg aaatcattca atactcgcac tatcgaaagt tcaccagcca
    26941 accgcagcac gtcctgcata cgtcgtgtct gcggtttttc tttttcgctt acattgtgtc
    27001 tggttcttcc ggccactcaa tatcaggtgc agttgatgta tcaacacggt tcagcaacac
    27061 ccgatacttt ttctaggctt ccagcaatga ggtttcttcc tccgttgcaa tttccagatc
    27121 tgcagcatcc tgaagtgggg cgcaccacgc aaacatcaca aaatattgtc gctgcgatga
    27181 acctgtacaa taaataatga atcacggtac gtttctgtgt ataacgtact gtagttttct
    27241 ttacgattta tagctgtact ggtgagccat gagtaatctg aatccatgca tggcgtgtgg
    27301 tgccgttgtg cattttctgc gtctctttta ctggtccgaa gccgatgatg ctgaagcagt
    27361 cctctcctct agcctcatcc ctgtatagca cgcatcaaat aatctgggat atcatttcgt
    27421 agttttacaa tatcaatatc cgaatcgata tgtttctggt aaaacttgtc cgcaagcaag
    27481 tttgctatgg tatctgatgt acgatttttt acatcccggc tcataccatc acaaagagca
    27541 tccattttct tgccaaaaga agcactcccc attattacat gattaataga actttgtgct
    27601 gccttaacat caagggttag ttgattctcc tggctcacaa gaaaaaagac tctttgcctc
    27661 tcttcagtgg aaatttttgc atttttttga aaacagtaat tatcaatctc ctggttgatc
    27721 acccttgagg gatatactat ccgttcatct tttatccact catcaatatc tttagtactc
    27781 tgccttctca ccgtttccag tatattgccg ctaaatttgc gaacagaaaa gctaatagtc
    27841 cgttctccaa ttgaacattt tacgacctca tcattcttcc ctcggaaaga gttcctgaag
    27901 gagttctctc tatggcaact tataccaaaa gagctgaaag acatacctgt agtattcatc
    27961 ggcattataa ttccttttta taaaaaagtg tttacaatat tgcatacttg cgtttatt
//
LOCUS       DAZKIP010000052        27277 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZKIP010000052 DAZKIP010000000
VERSION     DAZKIP010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.052"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            132..1178
                     /locus_tag="ACHOUK_002489"
     CDS             132..1178
                     /locus_tag="ACHOUK_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFP9897919.1"
                     /translation="MKTVNVALLALIISATSSPVVLAGDTIEAAATELSAINSGMSQS
                     EIEQKITRFLERTDNSPAAYTYLTEHHYIPSETPDTTQTPTVQTDPDAGQKTVAATGD
                     VQTTARYQSMINARQSAVTDAQQTQITEQQAQIVATQKTLAATGDTQKTLAATGDTQN
                     TAHYQEMINARLAAQNEANQRTATEQGQKMNALTTDVAVQQQNERTQYDKQMQSLAQE
                     SAQAHEQIDSLSQDVTQTHQQLTNTQKRVADNSQQINTLNNHFSSLKNEVDDNRKEAN
                     AGTASAIAIASQPQVKTGDVMMVSAGAGTFNGESAVSVGTSFNAGTHTVLKAGISADT
                     QSDFGAGVGVGYSF"
     gene            1263..1685
                     /locus_tag="ACHOUK_002490"
     CDS             1263..1685
                     /locus_tag="ACHOUK_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP9897920.1"
                     /translation="MMNSSIKSFSLLAVILLAGCSSPTSRIADCQAQGVSHDTCYLAE
                     QQRQAAILSASEAQAFKNAEAAQHAQAAKKAIYKGFGMTFRMSSKNFAYLNDSLCAID
                     EDNKDATVYQSGLYNVIVYHHTGKVALMKEGQFVGYLK"
     gene            1682..1885
                     /locus_tag="ACHOUK_002491"
     CDS             1682..1885
                     /locus_tag="ACHOUK_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9897921.1"
                     /translation="MKEQRKIPLTHIMIIGAFIFAFLQVVLLASLVHAVNVNNEIQEG
                     LFQSGRIMVESLQHILSVQTGIH"
     gene            complement(1889..2296)
                     /gene="cueR"
                     /locus_tag="ACHOUK_002492"
     CDS             complement(1889..2296)
                     /gene="cueR"
                     /locus_tag="ACHOUK_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFP9897922.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     NQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(2293..3468)
                     /locus_tag="ACHOUK_002493"
     CDS             complement(2293..3468)
                     /locus_tag="ACHOUK_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HFP9897923.1"
                     /translation="MNGQLQYNRGLTEPRLPRSALAVRVTAVMLLCFLGWAWYFQLDE
                     VTTGSGTVEPSGREQVVQSLEGGILYHLDVKVGDIVEQGQPLAQLNRTKTESDVQEAM
                     SRLYAALATSARLRAEVSNKPLVFPDELNKFPELIESETALYNTRRDGLNKATTGLTQ
                     GISLVNRELAMTQPLVKQGAASSVEVLRLQRQANELENKLSDVRTQYYVQAREELAKA
                     NAEVETQRSVIRGREDSLTRLNFTAPVRGIVQDIDVTTVGGVIAPGGKLMTIVPLDEQ
                     LLIEAKISPRDVAFIHPGQKSLVKITAYDYSIYGGLPGEVAVISPDTVQDEVRRDVYY
                     YRVYIRTFSNHLENKSKQQFPIFPGMVATVDIRTGKKSVLDYLLKPFNKAQEALRER"
     gene            complement(3465..5627)
                     /locus_tag="ACHOUK_002494"
     CDS             complement(3465..5627)
                     /locus_tag="ACHOUK_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HFP9897924.1"
                     /translation="MKKNAQSVEAWLEAMIAVARYYRLDFSQENVRATVNWERDSKRE
                     ELLTDMARQLGMGLRLVEFSADSLNPWRLPLIAVFDNQQIGVITRRDNHDNISVQFSG
                     DEGLETTLNVADIEDKIVELALLRPLSAIPDARVDDYIRPYQANWFWSLSLKDWRRYG
                     DIMLASLVANVLALAAMIFSMQVYDRVVPAQSYPTLWVLFAGVMMAILFEFCMRMVRT
                     HLSDVIGKRADLRISDRVFGHALRLKNNVRSKSTGSFISQIRELESVRELITSTTIGA
                     VADLPFFLLFVFILWMIGGWLVLVVLLALPLLVIPGLLVQRPLARLANEGMRESAVRN
                     ATLVEAVQSIEDIKLLRAEQRFQNQWNHTNDVASSISMKQRFLTGLLLTWTQEVQSIV
                     YVVVLLVGCFMVMNGDMTTGALVGTTILASRTIAPLSQISGVLSRWQQAKVARNGLDE
                     LMKRPVDQPEHGKLVHKAVLHGNYQFSNAVFYYDEEEKIADVAIGKLNIQAGEKIAIL
                     GRNGAGKSTLLQMLAGMRIAQQGQVLLDNISIGQLDPADLRRDMGLLSQTGRLFFGSL
                     RENLTMGMPEASDEDIERALTLSGALPFVQKQKNGLNYMIQEGGFGLSGGQRQTLLLA
                     RLLISQPNIVLLDEPSASLDEMAEAYLIEQLKQWIGHRTLIIATHRTAMLQLVDRIIV
                     MDQGRIVMDGAKEAILREQGEPTARRVVLQEKNKGSAA"
     gene            complement(5631..21368)
                     /locus_tag="ACHOUK_002495"
     CDS             complement(5631..21368)
                     /locus_tag="ACHOUK_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFP9897925.1"
                     /translation="MDINTIATDDIINASEKGADLQISGTSNQPAGTTITLALNGQNY
                     TATTDAAGNWSTTVPASAVGALGEASYTVTANVTDSAGNSNSASHNVQVNTALPGVTI
                     NPVATDDIINAAESGNAQTISGQVTGAAAGDTVTVTLGGKTYTATVQGNLSWSVDVPA
                     ADIQAIGNGNLTVNASVTNGVGNTGSGSRDITIDANLPGLRVDTVAGDDVVNSIEHAQ
                     ALVITGSSSGLAAGAALTVVINTVTYAATVLADGTWSVGVPAADVSNWPAGTVNITVS
                     GTNTAGTTSTITHPVTVDLAAVAISINTVSGDDVINAAEKGADLTLSGSTSGVEVGQT
                     VTVTFGGKTYTATVAGDGSWTTTVPAADLSVLRDGDATVQASVSTINGNTASATHAYS
                     VDATAPTLAINTIATDDILNAAEAGNPLTISGSSTAEAGQTVTVTLNGVTYSGSVQAD
                     GSWSVSLPTADLSNLTASQYTVSASVSDKAGNPASANHGLAVDLTVPVLTINTVSGDD
                     IINAAEHGQALVISGSSTGGEAGDVITVTLNSKTYTTMLDASGNWSVGVPAADVTALG
                     SGPQTITAAITDAAGNSDDASRTVTVNLAAPTIGINTIATDDVIKATEKGADLQITGT
                     SNQPAGTTITVTLNGQNYTATTDSNGNWSATVPASAVSALGEANYTVTANVTDTAGNS
                     NSASHNVLVNSALPAVTINAVATDDIINAAESGNAQTISGQVTGAAQGDTVTVTLGGN
                     TYTATVQSNLSWSVDVPAADIQALGNGDLTVNASVTNGVGNTGSGSRDITIDANLPGL
                     RVDTVAGDDVINSIEHNQALVITGSSSGLTAGTALTVEINNVTYGATVLADGTWSLGV
                     PAVDVSNWPAGTVNITVSGTNSAGTTSTITHPVTVDLAGVAITINTLSGDDVINAVEK
                     GETLVVSGSTSGVEAGQTVTVTFGGKNYTTTVEANGSWTVNVPPADLAALPDGAGNVQ
                     ASVSNINGNSAQADRAYSVDATAPLVTINTIASDDILNVSEAGAGITISGTTTAQAGQ
                     TLTVTLNNNTYQTTVLADGTWSVNVPAADLSGLTASSYTVTATVSDKAGNPASADHAL
                     VVDITAPDLTINTVAGDDIINAIEHGQALVVSGTSTGAAAGDVVTVTLNGKNYTTTLD
                     ASGNWSVGIPAADVTALATGSQTITASLSDRAGNSDSTTHDVTVDLSGPTLTINTVSG
                     DDIINAAEIVVAQTISGQVTGTAVAGNTVIVTIGGNQYNATVQSDLSWSVSVPANVLQ
                     ALGNGELTISASLTNSANNTGTATHDIVIDANLPGLRVDTVAGDDVINSIEHTQALVI
                     TGSSSGLAAGAALTVVINSVTYGATVLADGSWSVGVPVADVTNWPAGTVNIAVSGTNT
                     AGTTTSISHPVTVDLAAVAITINTLSTDDVINAAEKGSDLQLSGTTSGVEAGQTITVI
                     FGGKSYTTTVAADNTWGLTIPAVDVATLPDGAANVQASVSNVAGNSTQATHAYSVDAT
                     APSVTINTIATDDILNAAEAGSALTISGTSTAEAGQTVTVTLNGVNYSGNVQADGSWS
                     VSVPTGDLASLTASSYTVNASVSDKARNSASATHNLTVDLAAPVVTINTVAGDDIINA
                     TEHGQAQIISGSATGATTGNTVSVTIGTTTYTTVLDANGNWSIGVPASVISALAQGDV
                     TITATVTDSAGNSGTASHTVTVALGAPVLAINTIAVDDIINAAEKGADLAITGTSNQP
                     AGTQITVTLNGQNYTTTADASGNWSVTVPASRVSALGEATYTVTAAATDADGNSGSAS
                     HNVQVNTALPGVTINVVATDDIINAAEAGVEQTISGQVTGAAAGDTVTVTLGGATYTA
                     TVQANLSWSVDVPASALQELGNGELTISASVTNSVGNTGNGTREITIDANLPGLRVDT
                     VAGDDVVNIIEHGQALVITGSSSGLAAGSNVTLTINGQTYVAAVLADGTWSVGVPAVD
                     VSAWPAGSVTIAASGSTSAGNPVSVTHPVTVDLSAVAVSINAITADDVINAAEKGAAL
                     TLSGSTSGVEAGQTVTVTFGGKTYSATVAANGSWSTSVPAADMAALRDGDASAQASVS
                     NVNGNSATTTHAYSVDASAPTVTINTIAGDDILNAAEAGAALTITGSSTAEAGQTVTV
                     TLNGTNYTGTVQTDGSWSVSVPSADLSTLTASNYTVNAAVSDKAGNPASVNHNLTVDT
                     SVPVVTINTVAGDDVINATEHAQAQIISGSATGAATGSTVTVTIGTNTFTTVLDASGN
                     WSVGVPASVVSALANGTVTINASVTDAGGNSGSATHQVTVNTGLPTITFNAISGDNIL
                     NADEKGQPLTISGGSTGLATGAQVTVTLNGHNYSATTDASGNWTLTVPVSDLAALGQA
                     NYTVSASATSAAGNTASSQANLLVDSGLPDVTINTVAGDDIINAAEAGADQTISGVVT
                     RAAAGDTVTVTLGGNTYTATVQSNLSWSVSVPTADLQALGNGDLTITASVTNANGNTG
                     SGTRDITIDANLPGLRVDTVAGDDIVNSIEHGQALVITGGSSGLNAGAVLTVTINSVA
                     YSATVQADGSWSVGIPAANVSAWPAGPLTVEVDGQSSANNPVSVSHPFTVDLTAVAIS
                     INTVASDDVINAAEKGTNLTLSGSTSGIESGQTVTVTFGGKTYTASVAANGSWSVNVP
                     AADLATLPEGAANVQASVSSASGNSASATHAYSVDASAPTLTINTIASDDILNAAEAG
                     SPLTISGTSTAETGQTVTVTLNGATYTGTVQADGSWSVSVPTSALGALNASNYTVSAT
                     VNDKAGNPGSASHNLAVDTTAPVLTINTVAGDDIINDAEHAQALVISGTSSGGEAGDV
                     VSVVLNGKTYTTTLDASGNWSVGVPAADVTALGSGAQTITASVSDRAGNSDDASRTVT
                     VSLSAPVISINTIAGDDVINATEKGSDLALSGTSDQPAGTAITVTLNGQNYSATTDAS
                     GNWSVTVPASAVSALGEATYSVTASVTNAQGNSSTASHNVQVNTALPGITINPVATDD
                     IINASEAGSAQTISGQVTGAAAGSTVTVELGGKTYTATVQADLSWNVSVPAADWQALG
                     NGELTVNASVTNAVGNTGSGTRDITIDASLPGLRVDTVAGDDVVNIIEHAQAQVITGS
                     SSGFAAGTALTVVINNQTYAATVLANGSWSVGVPATDVSNWPAGTLNITVSGANSAGT
                     QTSITHPLTVDLTAVAISMNSITSDDAINAAEKGAALTLSGSTSGVEAGQTVTVTFGG
                     KTYTTTVAANGSWSTTVPAADLAALRDGDASAQVRVTNVNGNSATATHEYSVDSAAPT
                     VTINTIASDNIINASEAAAGVTVSGTSTAQTGQTLTVTLNGTNYQTTVQTDGSWSLTL
                     PASDLTALANNGYTLTATVSDLAGNLGSASKGVTVDTTAPVISFNTVAGDDVINNVEH
                     IQAQIISGTATGAVAGDRLVVTIAGQQYVTSTDASGNWSVGVPASVISGLADGTVTIS
                     ATITDSAGNSSTQTHNVQVNTAAVSLSVSTISGDNLINAAEAGSALTLSGTGTNFATG
                     TVVTVLLNGKGYSATIQSNGSWSVNVPAADVAALSDGTSYTVSASAQDSAGNSATASR
                     SVAVDLTAPVISINTVSTDDRLNAAEQQQPLTLNGSTSAEVGQTVTVTFGGKTYTATV
                     AANGTWALNVPAVDLAALGQGAQTITASVNDRAGNPGQATHALTVDTVAPTVTIATVA
                     GDDIINNAEQLAGQTISGTTTAEVGQTVTVTFNGQTWSATVGSGGSWSVFIPAQQFAG
                     LSDGSYTISATVSDQAGNPGSASRGVTLNGDVPTVTINTFAGDDVVNAAEHGSSLVIS
                     GTTTAPVGQTLTLTLNGKTYTTTVQTGGSWSYTLGSADVTALADGNAYVINASVSNAI
                     GNTGSSNHTITVDLSAPAMGINIDSLQADTGLSASDFITSVSPVVVNGSLTAALASNE
                     TAQISIDGGTTWTTLTVTGTTWRYNDSRTLTDGNYLYQVRVIDAAGNVGATDSQNVVI
                     DTTAPDPAVKTIAISAITTDMGLITNDFVTSDTTLAVSGTLGATLSAGEFAQISLDGG
                     VTWTTLTVVGTSWSYADGHTLTDGTWNYTVRVVDLAGNVGQTATQNVVVDTTSPEAAK
                     SITITGISDDTGTSSSDFITSDTTLTVRGVLGAALGANEFAQISTDNGATWVNVTVAA
                     DSLNWSYVDGRTLTNGTTTWQVRVVDLAGNVGATSSQSALIDTVNPAQVLTIASISTD
                     TGSSATDFITSDTMLTLTGSLGAGLASGEVAQISLDSGATWTTLTTNGTQWTYTDSRT
                     LTDGSYVYQVRVLDLAGNTGPVVSKTVVVDTINPTATPTIVSYTDDVGQRQGTLSSSQ
                     ATDDTTPLLNGVLSAPLASGEVVYLYRNGLLLGAVTMVGALNWTYSDSGLVSGAYTYS
                     ARVVDLAGNITSSSDFVLTVDTSIPTTLAQITSQTTRDTTPIISGVITAALASGQYVE
                     VVINGKTYTSEPGGAVVVDPAHNTWYVQLPDTDALTVSATAYTVTAQVKSSAGNGNNA
                     NISNGTVTVNAAIDYTPTWTTASKTTAWGLTYGLDSHGMWTVLANQQVMQSTDPLTWS
                     KTALTLYQSGNNYATSSIADYDRNGTGDLFITRDDYGTGYINGFTNNGDGTFSSAIQV
                     TVGTLTWYGSIVAFDKEGDGYLDFWIGDAGGPDSNTFLWNNAGTLVGNSTTSNSGGSA
                     TVGGAVTGYLSLNEGSGVDLNNDGRIDLVQHTYNLNNYYTLSSLINQGNGTFVWGQNT
                     TNTFLSGAGSGAMSSSVSMTWADFDGDGDMDLFLPASQGRANYGSLLFNTNGVLGSPV
                     AVGATATTYASQFSLAVDWNHDGLMDIARIAQTGQSYLYTNVSNASNWTQSALGGSQS
                     GTTSGVAAMDYDWDGAVDVLVSKQSGSVFLSRNTNTVSYGTSLHLRITDPNGINVYYG
                     NTVKLYNSAGVLVATQIINPQSGMGVNDTSALVNFYGLNAGETYNAVLIKSTGTTASN
                     IDQTVNTSWGGLQATDATHAYDLSAEAGTASNNGKFVGTGYNDTFFATAGTDTYDGSG
                     GWVYSSGTGTWLANGGMDVVDFRLSTVGVTANLSSTAAQATGFNTSTFTNIEGISGSN
                     FNDILTGSSGDNQLEGRGGNDTLNIGNGGHDTLLYKLLNASDATGGNGSDVVNGFTVG
                     TWEGTADTDRIDIRELLQGSGYTGNGKASYVNGVATLDAQAGNIGDFVKVTQSGSDTI
                     VQIDRDGTGGTFATTNVVTLTGVHTDLATLLANHQLMVV"
     gene            complement(21405..25790)
                     /locus_tag="ACHOUK_002496"
     CDS             complement(21405..25790)
                     /locus_tag="ACHOUK_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFP9897926.1"
                     /translation="MSLIIDVISRKTSVKQTLINPGDVTVVIYEPSVVQVHAQASAVA
                     RYVREGNDLLIYMQDGTVIRCNGYFLQAANTAEQSELVFADGQQLTHITFADTAAGGL
                     APVELTAQTTAIESIAPFLDTVAQTSAFPWGWLAGAAVGGGALGALLASGGDGDSKTE
                     VINNPTPPAEPGNATPSFLVTDNQGDQRGILATNDITDDTTPTFSGSGQAGATIQIKD
                     SNGNTIASTQVDNNGHWSVSLPTQSAGEHTWSVVQIVGSTITDAGSITLTIDNSQASV
                     QVATTAGDNIINASEQAAGFTLSGTSSHLAQGTELTVTLNGKTYTTSVGANGAWSVQV
                     PTADAQALGEGNQAVLVSGKDATGNTVTGAQLLTVDTQPPTLAINTIAQDNIISAAEH
                     NVALVLSGTSNAEAGQTVTLTVNGKSHTATVGSDGTWQVTLPATEVQALAEGNYAVNA
                     SVSDRAGNTTSHSANFTVDTSAPVVSVNTVAGDDILNNAEQAVAQIISGQVSGASPGD
                     TVTVKLGTHVLTGIVLADGSWNVALDPAVTRTLDRGANTIFVTVTDAAGNTGAASRAI
                     TLVGVSPLITINTVSGDDIISGAEKGAPLTLTGSTQQAETGQTVTVTLAGQSFTTTVQ
                     ADGSWSLTVPAAAMGNLPDGAVAITASVTDLSGNTGNTSRTITVDSQAPALSIDPLTA
                     DNIINAAESGQDLPITGTTDAQPGQTVTVTLNGQTYQGVVQPDGTWSVTVPAANVGAL
                     ADGNATVTASVNDVAGNPSSVSRVALVDATPPVVTINPVATDNVINTPEHAQAQIISG
                     TVTGAQAGDIVTVTLNNVDYTTVVDGSGNWSLGVPASVVSGLADGSYPVSVSVTDKAG
                     NTGSQSLTVTVNTAAPLIGINSIAGDDVINASEKGADLQITGTSDQPVNTAITVTLNG
                     QNYTTTTDASGNWSVTVPASAVTALGQANYTVTAAVTSDIGNSATASHNVLVDSALPG
                     VTINPVATDDIINAAEAGVAQTISGQVTGAEDGDTVTITLGGNTYTATVGSNLTWSVD
                     VPAADIQALGNGDLTVNASVTNQNGNTGSGTRDITIDANLPGLRVDTVAGDDVVNIIE
                     HGQALVVTGSSSGLAESTPLTVTINNVEYTTAVQADGSWSVGVTAAQVSAWPAGTVNI
                     AVSGESSAGNSVSITHPVTVDLTPAAITINTIATDDVINAAEKGADLTLSGTTTNVEP
                     GQTVTVTFGGKNYTASVASDGSWTATVPAADLASLPEGSASALASVSNINGNSASAVH
                     NYSVDSSAPTIIINTVASDNIVNASEADAGVTVSGSTTAEAGQIVTITLNSPTVQTYQ
                     ATVQADGSWSINIPAADLEALTDGSHTLTATVNDKAGNPASTTHNLAVDLTVPVLTIN
                     TIAGDDIINATEHGQALVISGSSTGGEAGDVVTVTLNSKTYTTTLDASGNWSVGVPAA
                     DVTALGSGPQTVTATVTDAAGNSDN"
     gene            complement(25893..27248)
                     /locus_tag="ACHOUK_002497"
     CDS             complement(25893..27248)
                     /locus_tag="ACHOUK_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HFP9897927.1"
                     /translation="MARFQFKNRKNNGLIFFISFMVMGEAAIAAPLPQWANAPAVTPV
                     AQLSLQESILRAFARNPGVTQQAAQIGIGEAQIDEAKSAWYPHVGLTGNAGPSRQTDS
                     SGRLDNNVSYGITLTQLVYDFGKTNNDINLQTAARDSYRFKLMATLTDVAEKTATAYM
                     EVSRYQALCDAAQRNIHSLENVYNMAALRANAGLNSSSDELQAQTRIAGMRSTLEQYQ
                     AQMASAKAQLAVLTGVQPEAIAAPPAELAEQPVSLKNIDYQSIPLVLAAENLRQSAQY
                     GVEKTKAQYWPTLSIQGGKTRYQTSDRSYWDDQLQLNVNAPLYQGGAVSAQVQQAEGQ
                     QKISASQVEQAKLDVLQRASVAYANWTGARGREEAGLAQSESAHKTRDVYQNEYKLGK
                     RSLNDLLTVEQDVFQAQSAEINANYDGWVAAVNYAAAVNNLIPLAGIKQGLYNDLPDL
                     K"
BASE COUNT         5090 a         8035 c         7905 g         6247 t
ORIGIN      
        1 aaatgcgctt tacatcacac aaatggcggc gtagatttcg attaaattgc aacgcagttt
       61 atttcttaaa acaatattat ttgtttctta tagaaacatt aatacgactt attttgaaca
      121 agagaaaatg aatgaaaact gtaaacgtag ctttactggc actcataatt tcagcaacat
      181 ccagccctgt tgttttagct ggtgatacca ttgaagcggc ggcaacagag ctttcagcca
      241 ttaactctgg catgtcgcaa tcggagattg agcagaagat tacccgcttt ttagaacgca
      301 cagacaacag ccccgctgcg tatacctatt tgactgaaca tcactacatc ccttctgaaa
      361 cacctgatac cactcagact cccactgtcc agacagatcc tgacgcagga caaaaaaccg
      421 ttgccgctac aggtgatgta cagacaactg cccgttatca gagcatgatc aacgcccgac
      481 agtctgcggt aactgacgcc cagcaaacgc aaattacaga gcaacaggcg cagatcgtag
      541 ccacacaaaa aacgctcgcc gcgactggag atacgcaaaa aacgctcgcc gcgactggag
      601 atacgcaaaa taccgcgcat tatcaggaaa tgattaatgc cagactggcg gctcaaaatg
      661 aggctaatca gcgcaccgcc actgaacaag ggcagaaaat gaatgcgctg acaaccgatg
      721 tggcagtaca acagcaaaat gaaaggactc aatacgataa acaaatgcaa agtctggcgc
      781 aggagtctgc ccaggcacat gaacaaattg acagcctgtc acaagacgta acccaaacgc
      841 accaacagtt aaccaacacc caaaaacggg ttgcagataa cagccagcaa attaacacgc
      901 tcaataacca tttcagttcg ctaaaaaacg aagttgatga caatcgtaaa gaagccaatg
      961 cgggaactgc atctgccatc gctatcgcct cacaaccaca ggttaaaacc ggtgacgtga
     1021 tgatggtgtc agcgggagcg ggaaccttca acggtgaatc tgcggtgtct gtcggaacat
     1081 catttaatgc cggaacgcat acggtactta aagccggtat ttctgcggat acacaatctg
     1141 atttcggcgc aggtgtcggc gtgggatatt cgttctaata tttcaatcct caatataaat
     1201 aagagcaagg aagcttgccg ggttcacctc ttcattaatt tgtacattat ttaaggttaa
     1261 caatgatgaa tagctccatt aaatcgtttt ccctgctggc ggttatatta ctggctggct
     1321 gtagttcacc cacttcccgc atcgcagatt gccaggcgca gggcgtcagt catgacacct
     1381 gttacctcgc agaacagcag cgtcaggcgg ctattttaag tgcatccgag gcacaggcat
     1441 ttaaaaatgc agaagccgca caacacgccc aggcggcaaa gaaagccatt tataaaggat
     1501 ttggcatgac ctttagaatg agcagtaaaa actttgctta tctcaatgat tcattatgtg
     1561 caattgatga agacaataaa gatgccactg tttatcagtc aggtctatat aacgtcattg
     1621 tttatcatca cacaggaaaa gtcgccttaa tgaaagaagg ccagtttgtg ggttatttaa
     1681 aatgaaggag caaaggaaaa tacccctgac gcatattatg attatcggtg cgtttatttt
     1741 tgccttcttg caagtagtat tattagcctc cctggttcac gctgtgaatg ttaacaacga
     1801 aatccaggaa ggcttatttc agtcggggcg cattatggta gaaagtttgc agcatattct
     1861 ttcggtgcaa acggggattc actgattttc accccgcccg atgatgacag cagccggaga
     1921 gattttcgat aatcgggcag tcggcgctgt catcaccggg gcaggcattc gccagcgcca
     1981 gtagctggtt gcgcatagat tgcagttctt cgatatgccg ttcaatctcc gccaccttct
     2041 ccagcgtgcg acgtttgacg tcggcactgt gacgctgcgg gtcgttaaac agattcacca
     2101 gctcgccgct ctcttccagg ttaaagccca cctggcgcgc ctggcgcagt aaggtcaatt
     2161 cgttgagatg ctgctgcgtg taggttcgat aaccattttc gctgcgcatc ggcggcgtca
     2221 ccagcccctt ctcttcatag aagcgaatgg ctttgctggt caggccggta atttttgcta
     2281 catcgctaat gttcatcgtt cgcgcaacgc ctcctgtgct ttgttaaagg gtttcaacag
     2341 ataatccagc acgctttttt tgccggtgcg gatatcgacc gtcgccacca taccggggaa
     2401 tatcggaaac tgctgtttgc ttttgttttc cagatggttg ctgaaagtgc gaatgtacac
     2461 ccgatagtaa taaacatcgc gccgcacttc atcctgcacg gtgtctggtg atatcaccgc
     2521 cacttctccc ggcaggccgc cgtagatgga gtaatcgtag gcagttattt tcaccagcga
     2581 tttttgcccc gggtggataa acgccacatc gcgcggggag atttttgcct caatcagcag
     2641 ttgctcatcc agcggcacga tggtcatcag tttaccaccg ggggcgatga cgccgccaac
     2701 cgtggtgaca tcaatatcct gaacaatgcc tcgcaccggc gcggtaaagt tgagcctggt
     2761 gagagagtct tcccgtccac ggatcaccga acgctgggtt tccacttccg cattggcttt
     2821 tgctaactct tcgcgcgcct gaacgtagta ttgcgtgcgc acgtcgctga gtttgttttc
     2881 cagttcattg gcctggcgtt gcaggcgcag tacttcgacg ctgctcgctg ccccctgttt
     2941 taccagcggc tgggtcatcg ccagttcgcg attgaccagg ctaatcccct gcgtcagccc
     3001 ggtggtggct ttgttcaacc cgtcgcggcg agtgttgtac aacgccgttt ccgactcgat
     3061 aagttcgggg aatttattca gttcgtcagg gaagaccagc ggtttattgc tcacttccgc
     3121 ccgcagccgc gccgaggttg ccagcgccgc gtacagccgc gacatcgctt cctggacgtc
     3181 ggattccgtt ttggtgcggt taagctgcgc cagcggctgg ccctgttcga caatatcgcc
     3241 aactttcacg tcgagatgat acagaattcc gccttccagc gactgcacca cctgttcgcg
     3301 cccggaaggt tctaccgtgc cgctgccggt ggtgacttca tcaagctgga agtaccacgc
     3361 ccagccgaga aaacagagca gcatcacagc ggtaacgcgt accgccagcg ccgaacgcgg
     3421 caggcggggt tcagtcagac cacggttata ttgcagttgt ccgttcatgc cgcagacccc
     3481 ttattcttct cctgcaacac cacccgtcgc gctgtgggct cgccctgttc acgcaaaatg
     3541 gcctctttcg cgccgtccat cacaatccgc ccctgatcca tcacgattat cctatccacc
     3601 agttgcagca tcgccgtgcg gtgagtggcg ataatcagcg tgcgatgccc aatccactgt
     3661 ttcagttgtt cgattaaata cgcttctgcc atttcgtcca gtgaggcgct gggttcgtcg
     3721 agcaggacga tattcggctg agagatgagt agccgcgcca gcagcagcgt ttgccgctga
     3781 ccaccagaga ggccaaatcc cccttcctga atcatgtagt tgaggccgtt tttctgcttc
     3841 tgcacaaagg gcagcgctcc gctgagggtt aacgcgcgtt cgatatcttc atcgctggct
     3901 tccggcatcc ccatggtgag gttttcccgc agcgaaccaa agaacagtcg cccggtctgg
     3961 ctgagtagcc ccatgtctcg ccgcagatct gccggatcaa gctgcccgat actgatgtta
     4021 tccagcaaca cctgcccctg ctgggcgata cgcattccgg cgagcatttg cagtagcgtc
     4081 gatttacccg cgccgttgcg cccgagaatg gcgattttct caccagcctg aatgttgagt
     4141 ttgccaatcg ctacatcggc aatcttctct tcttcgtcgt aataaaacac cgcgttgctg
     4201 aactgataat taccgtgcag caccgcttta tgcaccagtt tgccgtgttc cggctgatcc
     4261 accgggcgct tcatcagttc atcaaggccg ttacgtgcta ctttcgcctg ctgccagcgc
     4321 gagagcacgc cggatatctg cgacagcggc gcaatggtgc gcgaagccag gatcgtcgtc
     4381 ccgaccagcg cgccggtggt catgtcgccg ttcatcacca taaagcagcc caccagcagc
     4441 accaccacgt agacgatgga ctgcacttcc tgggtccagg tgagcagcag cccggtgaga
     4501 aaacgttgct tcatgctgat ggacgaggcc acgtcgttgg tgtggttcca ctgattctga
     4561 aaacgctgtt cggcgcgcag cagtttaata tcttcaatcg actgcacggc ttccaccagc
     4621 gtagcgttgc gtaccgctga ctcgcgcatt ccttcgttcg ccagccgcgc cagcgggcgt
     4681 tgcaccagca ggccggggat caccagcaac ggcagcgcca gcaaaaccac cagcaccagc
     4741 cagccgccga tcatccacaa aataaagaca aacagcagga agaatggcag gtccgccacc
     4801 gcgccgatgg tggtggacgt aataagctcc cgcactgatt ccagttcgcg gatctgcgag
     4861 ataaacgatc cggtggattt cgatcgcacg ttgtttttca gccgtagcgc atgaccaaaa
     4921 acgcgatccg aaatacgcag gtctgcccgc tttccgatca catcagatag atgcgtacgc
     4981 accatgcgca tacagaactc aaacaggatc gccatcatca ccccggcgaa aagcacccat
     5041 agcgtcgggt acgattgcgc cggaaccacc cgatcgtaaa cctgcatcga gaaaatcatc
     5101 gccgccagcg ccagcacgtt cgccaccagt gacgccagca taatatcgcc gtagcgccgc
     5161 cagtctttta gcgaaagact ccagaaccag ttcgcctgat acgggcgaat atagtcatcc
     5221 acccgcgcgt cagggatggc gctgagaggg cgcagcagcg ccagttcgac gattttatct
     5281 tcaatatccg cgacgttaag tgtagtttcc agcccttcgt caccgctgaa ctgaacgctg
     5341 atattgtcat gattgtcgcg acgggtgatc acgccaatct gctgattatc aaacaccgcg
     5401 atcagcggca gacgccaggg gttaagcgaa tcggcggaaa actccaccaa ccgcaacccc
     5461 atccctaact gccgcgccat gtcggtgagt aactcctccc gcttactgtc gcgctcccag
     5521 ttgacggtcg cgcgcacatt ctcctgagaa aaatcgagcc gataatagcg cgccacggcg
     5581 atcatcgcct ccagccaggc ttccacggac tgggcatttt ttttcattat ctacaccacc
     5641 atcaactgat gattcgccag caaggtggcg aggtcggtgt gcacgcccgt cagcgtgacc
     5701 acgttagttg tcgcaaaagt gccgcccgtg ccgtcgcggt cgatctgcac gatggtgtcg
     5761 ctgccgctct gggtgacttt gacaaagtca ccgatgtttc cggcctgcgc atccagcgtt
     5821 gccacgccgt tgacgtagct ggctttgccg ttgccggtgt agccgctgcc ctgtaacagt
     5881 tcacgaatat caatgcgatc ggtgtcggcg gtgccttccc atgtccctac cgtaaagcca
     5941 ttcaccacgt ctgagccgtt gccgccagtg gcgtcgctgg cgttgagcag tttatagagc
     6001 aaggtgtcgt ggccgccgtt gccgatgttg agcgtgtcgt tgccgccgcg cccttccagt
     6061 tggttatcgc cgctgctgcc ggtcagaatg tcgttaaaat tcgagccgga aatgccttcg
     6121 atattggtaa atgtcgaggt gttaaaaccg gtggcctgcg cggcagtgct gcttaagtta
     6181 gccgtcacgc ccaccgtcga aagccggaaa tcaaccacgt ccatcccgcc gttcgccagc
     6241 cacgttccgg tgccggagct gtacacccag ccgccggaac cgtcgtaagt gtcggtgcct
     6301 gcggtggcga agaaggtgtc gttatagccg gtgccgacga acttgccgtt gttgctcgcg
     6361 gtacccgctt cagcgctgag atcgtaagcg tgagtggcat cggtggcctg taaaccgccc
     6421 cagctggtgt tgaccgtctg gtcgatattg ctggcggtgg tgccggtgga tttgatcagc
     6481 accgcgttgt aggtttctcc ggcattcagc ccgtagaagt tgaccagcgc cgaggtgtcg
     6541 ttaaccccca tacccgactg cgggttgatg atttgcgtgg cgaccagcac tcccgccgag
     6601 ttgtacagct tcacggtatt gccgtaatag acgttaatgc cgttgggatc ggtgatgcgc
     6661 aggtgtagcg aagtgccgta gctcaccgtg ttggtgttgc ggctcaggaa cacgctgccc
     6721 gactgtttgg acaccagcac atccaccgcg ccgtcccagt cgtagtccat tgccgccacg
     6781 ccgctggtgg taccgctctg gctgccgccg agggccgatt gcgtccagtt gctggcgttg
     6841 ctgacgttag tataaagata cgactgcccg gtctgggcga tacgggcgat atccatcagg
     6901 ccgtcgtggt tccagtccac cgccaggcta aactggctgg cgtaggtggt tgccgttgcg
     6961 cccaccgcca ccgggctacc cagtacgccg ttggtgttga ataacagcga gccgtagtta
     7021 gctcttccct ggctggcggg caggaagaga tccatatcgc cgtcaccatc gaaatcggcc
     7081 caggtcatgg aaacgctgct gctcatagcg ccactgcccg ccccgctcag gaaggtattg
     7141 gtggtgttct gcccccagac aaacgtccca ttcccctggt tgatgagcga agacagcgtg
     7201 taatagttgt tcaggttata ggtgtgctga accaggtcga tcctgccgtc attgttcaga
     7261 tcgacgccag aaccttcgtt gagcgaaaga taccccgtca ccgccccgcc caccgtggcg
     7321 ctaccgccgc tgttcgacgt ggtggagttg cctaccagcg tgcctgcgtt gttccacagg
     7381 aaggtgttgg agtccggccc gccagcgtca ccaatccaga agtcgagata gccgtcgccc
     7441 tctttatcaa atgccacaat cgagccgtac cacgtcaggg tgccgacggt gacctgaata
     7501 gcgctggaaa aggtgccatc gccgttattg gtaaagccgt taatatagcc cgtaccgtag
     7561 tcatcacggg tgataaacag atcgcccgtg ccgttacggt cgtaatcggc aatggagctg
     7621 gtggcgtagt tgttgccgct ctgatacagc gtcagcgcgg tcttcgacca ggtgagtggg
     7681 tcagtcgatt gcattacctg ctggtttgcc agcaccgtcc acatcccgtg cgagtcgagg
     7741 ccgtaggtca gcccccaggc ggtggttttg ctggcggtag tccaggtcgg tgtgtaatca
     7801 atcgccgcgt taaccgtcac cgtgccgttg ctaatattgg cgttattgcc gttacccgcc
     7861 gaacttttta cctgcgcagt aacggtatag gcggtcgcgg aaactgtcag cgcatcggta
     7921 tccggcaact gtacatacca ggtgttgtgc gccggatcga ccactaccgc gccgcccggt
     7981 tcagaggtgt aggttttgcc gttgatcacc acttcaacat actgcccact ggcaagcgcg
     8041 gcggtgatca ccccgctaat aatcggcgta gtatcgcgcg tggtctggct ggtgatttgc
     8101 gccagcgtgg tcggaataga ggtatcgacc gtcaggacaa aatcactgga ggaggtgata
     8161 ttccccgcca aatccaccac tcgcgcgctg taggtatagg caccgctcac cagcccgctg
     8221 tcgctgtagg tccagttcag agcgccgacc atcgtcaccg cccctaacag cagcccgtta
     8281 cggtagaggt aaaccacttc accgctggca agcggcgcgg aaagtacacc gttcagcagc
     8341 ggcgtagtgt cgtcggtggc ctgcgaactg cttaatgtcc cctgccgctg cccgacatca
     8401 tcggtatacg acacaatcgt tggtgtggcg gtggggttaa tcgtatcgac caccaccgtt
     8461 ttcgacacca ccgggccggt gttccccgcc agatccagca cccgcacctg ataaacgtag
     8521 ctgccgtcgg tcagcgtgcg gctgtcggtg taagtccact gtgtaccgtt ggtggtgagc
     8581 gttgtccagg tcgcgccgct atcaaggcta atctgcgcca cttcgccgct ggcaagcccc
     8641 gcccccagcg aaccggtcag cgtgagcatg gtgtcgctgg tgataaagtc agttgccgaa
     8701 ctccccgtgt cggtgctgat gctggcgatg gtgagcacct gcgccgggtt aacggtatcg
     8761 atcagcgccg actggctgct cgttgcgcca acgttgcccg ccagatcgac cacccgcacc
     8821 tgccaggtgg tggtgccgtt ggtgagggtt cgtccgtcaa cgtaactcca gttcaggctg
     8881 tctgcggcga cggtcacgtt cacccaggtt gcgccgttgt cggtactgat ttgcgcgaac
     8941 tcattagcgc cgagcgccgc gcccaatacg ccgcgcacgg taagcgtggt gtcgctggta
     9001 ataaaatcgc tgctgctggt tccggtgtca tcgctgatac cggtaatggt gatacttttc
     9061 gccgcttccg ggctggtggt gtcgaccacc acgttttgcg ttgcggtctg cccaacgttc
     9121 cccgccagat ccaccacccg caccgtgtag ttccaggtgc cgtcagtaag cgtgtgacca
     9181 tcggcatagc tccagctggt gccaacgacg gttaacgtag tccaggtgac gccgccgtcg
     9241 aggctgattt gcgcgaactc accggcagaa agcgtcgccc ccagcgtgcc gctcaccgca
     9301 agcgtcgtgt cgctggtgac aaaatcgtta gtgatcaagc ccatatcggt ggtgatcgcg
     9361 ctgatggcga tggttttcac cgcgggatct ggcgcggtag tgtcgatcac cacattctgg
     9421 ctgtcggtcg cgccaacgtt gcccgctgcg tcaatcaccc gcacctgata gagatagttg
     9481 ccatcggtca gcgtgcggct gtcgttatag cgccaggtcg tgccggtaac ggtaagcgtg
     9541 gtccaggttg tgccgccgtc gatacttatt tgcgccgtct cgttactggc aagcgcggcg
     9601 gtgagcgagc cgttgaccac taccggactg acgctggtga taaagtcgct ggcgctaagg
     9661 ccagtgtcgg cttgcaggga atcgatatta atgcccatcg ccggagcgct gagatcgacg
     9721 gtaatggtgt gattactgct accggtgttg ccaatggcat tgctcaccga ggcgttaatc
     9781 acgtaggcgt tgccgtccgc cagcgcggtg acatcggcgc tgccgagggt gtagctccag
     9841 ctaccgcccg tctgcaccgt ggtggtgtag gttttgccgt ttaaggtcag ggtcagcgtc
     9901 tgcccgacgg gcgcggtggt ggtgccgctg atcaccagcg atgagccgtg ttccgctgca
     9961 ttcaccacat cgtcgcccgc aaaggtgttg atggtgacag taggtacatc gccgttcagc
    10021 gtcacgccac ggctggcgct gccggggttt ccggcctgat cgctaaccgt cgcgctaatg
    10081 gtgtagctgc cgtcgcttaa tccggcaaac tgctgcgccg gaataaacac cgaccagctt
    10141 ccgccgctgc caaccgttgc gctccaggtt tgcccgttaa aggtaacggt caccgtctgg
    10201 cccacttcgg cggtggtggt gccgctaatg gtctgcccgg caagctgctc ggcgttgttg
    10261 ataatatcgt cacccgccac cgtggcgatg gtgaccgttg gcgcaacggt atcgacggtc
    10321 agggcgtgcg tggcctgtcc ggggttaccg gcgcgatcgt tcacgctggc ggtaatggtc
    10381 tgcgcgccct gcccgagcgc ggccagatcc accgcaggca cgttcagcgc ccaggtacca
    10441 ttggcggcaa ccgtggcggt ataggttttg ccgccaaagg tgacggtgac cgtctgcccc
    10501 acttccgcgc tggtcgagcc gttaagggtt aacggctgct gctgttcggc ggcgttgagg
    10561 cggtcgtccg tcgaaacggt gttaatgctg ataaccggcg cggtgagatc caccgccacg
    10621 ctgcgcgagg ccgtggcgct gtttccggca ctgtcctgag cggaggcgct aaccgtgtag
    10681 ctggtgccat cactgagtgc cgcaacatcc gccgcaggca cgttcacgct ccagctcccg
    10741 ttgctctgaa tggtggcgct gtagcctttg ccgttaagca acacggtcac cactgtacct
    10801 gtcgcgaaat tagtgccagt gccgctcagg gtcagcgcac tgcccgcttc ggcggcgtta
    10861 ataaggttat cgccgctgat agtgctgacc gagagcgaca ctgccgccgt gttcacctgt
    10921 acgttgtgcg tctgcgtgct gctgttgcct gcgctatcgg taatggtggc gctgatggtc
    10981 accgtgccat ccgcaaggcc ggaaatcacg ctggcaggca cgccgacgct ccagttgccg
    11041 ctggcatcgg tgctggtgac atactgctgc ccggcgatgg tcaccaccag gcggtcgccc
    11101 gccaccgcgc ccgtggcggt gccgctgata atctgcgcct gaatgtgttc gacgttgtta
    11161 atcacatcat cgcccgccac ggtgttaaaa ctgatcaccg gcgcagtggt atcgacggtc
    11221 acgcctttgc tggcgctgcc aaggttaccc gccagatcgc tgaccgtggc ggtcagggtg
    11281 tagccgttat tagcaagtgc ggttaagtcg ctggcgggca gcgttaaact ccagctgccg
    11341 tctgtctgca ccgtggtctg gtagttagtg ccgttaagcg tgacggtgag cgtctgcccg
    11401 gtttgcgcgg tgctggtgcc ggacaccgtt acgcccgccg ccgcttcgct ggcgttgatg
    11461 atgttatcgc tggcaatggt gttgatggtc accgttggcg cggcgctatc gacgctgtat
    11521 tcgtgcgttg ctgtggcgct gttgccgttg acgttagtca cccgcacctg ggcgctggca
    11581 tcgccatcac gcagggccgc caaatccgcc gccggaacgg tggtgctcca gctaccgttt
    11641 gccgccaccg tggtggtgta ggttttgccg ccaaaggtga cggtgacggt ttgccccgct
    11701 tcgacgccgg acgtgctgcc ggagagcgtt aacgccgcgc ctttttcggc ggcgttaatc
    11761 gcatcatcgc tggtgatgct gttcatgctg atggcgacgg cggtgagatc aacagtcagc
    11821 ggatgggtaa tgctggtttg cgttccggca ctgttcgccc cgctaacggt aatattcagc
    11881 gttcccgcag gccagttact gacatccgtc gcaggaacgc cgacgctcca gctaccgttc
    11941 gccaacaccg tggcggcgta agtttggttg ttaatcacca ccgtcaacgc cgtgcctgcg
    12001 gcaaagccgg agctgctgcc ggtgatcacc tgcgcctgag cgtgttcgat gatattgacc
    12061 acatcatcac ccgcgacggt atccacccgc aggccaggca gactggcatc gatggtgata
    12121 tcgcgcgtgc cgctaccggt gttgccaacg gcgttggtca ccgaggcatt aaccgttaac
    12181 tcaccgttac ccagagcctg ccagtcggcg gcaggtacgc tcacattcca gcttaaatca
    12241 gcctggacgg tggcggtgta agttttaccg cccagttcaa cggtgacggt gctgcccgcc
    12301 gccgcaccgg ttacctggcc gctgatagtt tgcgcgctgc ctgcctctga agcgttgata
    12361 atatcgtccg tcgccaccgg attaatggtg atgcccggca gcgcggtatt aacctgcacg
    12421 ttatggctgg cggtgctgct gttaccctga gcattggtga cgctcgccgt cacgctgtag
    12481 gtcgcttcgc ccagcgcact gaccgccgag gcaggcacgg taacactcca gttgccggag
    12541 gcatccgtgg tggcgctgta gttttgtccg ttcagggtga cggtgatcgc cgtacccgca
    12601 ggctgatcgc tggtgccaga aagcgccaga tcagatcctt tttccgtcgc gttgatcaca
    12661 tcatcgcccg cgatggtgtt gatgctaatc actggcgcgc tgaggctgac ggtcacggtg
    12721 cggctggcgt cgtcgctgtt gcctgcccga tcgctgacgc tggcggtgat ggtctgcgca
    12781 ccgctaccca gcgccgtaac atctgccgcc ggaacgccaa cgctccagtt gccggaggca
    12841 tccagggtgg tggtgtaggt tttgccgttg agcacaacgc tcaccacatc gcccgcttcc
    12901 ccgccgctac tggtgccgga gatcaccagc gcctgcgcat gttcggcatc gttgatgatg
    12961 tcatcgcccg ccacggtgtt aatggtgaga accggcgcgg tggtgtctac cgccagatta
    13021 tggctggcgc tgccggggtt gcccgctttg tcattgaccg tggcgctgac ggtgtaattg
    13081 cttgcgttga gcgcgcccag ggctgaagtg ggaacgctga cgctccagct accgtccgcc
    13141 tgcacagtgc cggtgtaggt tgcgccgtta agggtgacgg tcaccgtctg cccggtttcg
    13201 gcggtgctgg tgccgctgat ggtgagcggg cttccggctt ctgcggcgtt aaggatatcg
    13261 tcgctggcga tggtgttaat ggtgagcgtc ggcgcgctgg cgtcaacgct atacgcatgg
    13321 gtcgccgagg cactgttacc gctcgcgctg ctaacgctgg cctgcacatt cgccgcgccc
    13381 tctggcagag ttgccagatc tgccgccgga acgtttacac tccagctccc gttggcggcg
    13441 acgcttgcag tgtaggtttt accgccaaaa gtgacggtga cggtttgccc gctctcaatc
    13501 ccgctggtac tgccggaaag agtcagattg gtgccttttt ctgcggcgtt aatcacgtcg
    13561 tcgctggcaa cggtgttgat gctgattgcc accgccgtta aatcgacggt gaacggatgg
    13621 ctgacgctga ctgggttatt ggcgctgctt tgcccgtcta cctccacggt taacggcccc
    13681 gcaggccagg cgctgacgtt tgccgccgga atgccaacgc tccagcttcc gtccgcctgc
    13741 accgtggcgg aatacgccac actgttgatg gtaaccgtca gcacagcacc tgcattcagg
    13801 ccgctactgc cgccggtgat caccagcgcc tgcccgtgct cgatgctgtt gacgatatca
    13861 tcgcccgcca cggtatctac gcgcagcccc ggcaggttgg catcaatggt gatatcccgc
    13921 gtgccgctcc cggtgttgcc attagcgttg gtgaccgagg cggtaatggt caaatcacca
    13981 ttgcccaacg cctggagatc ggctgtcgga acgctgacgc tccagcttaa gttgctctgt
    14041 accgtagcgg tgtaagtgtt cccgcccagc gtcacggtga ccgtatcgcc agcggcggcg
    14101 cgagtcacca ccccgctgat ggtttgatcg gcccccgctt cggcggcgtt gataatatcg
    14161 tcgcctgcca cggtgttgat ggtgacgtcc ggcaggccgc tgtcgaccag taaattcgcc
    14221 tggctgctgg cggtgttgcc tgctgcactg gtggcgctgg cgctgaccgt atagttggcc
    14281 tgacctaatg ccgccagatc gctcaccggc acggttaagg tccagttgcc cgatgcgtcg
    14341 gtggtggcgc tgtagttgtg accgttgagc gtgacggtga cctgcgcgcc cgtcgccagc
    14401 cccgtactgc cgccgctgat ggtcaacggc tggccttttt catcggcgtt caggatgtta
    14461 tcgccgctga tggcgttaaa ggtaatggtc ggcagcccgg tattgaccgt cacctgatgg
    14521 gtagcgctgc cgctgtttcc tccggcatcg gtgacgctgg cattgatggt caccgtgcca
    14581 ttcgccagtg ccgagacgac gcttgccgga acgccgacgc tccagttgcc gctggcatcc
    14641 agcaccgtgg taaaggtatt tgtgccgata gtcaccgtca cggtgctacc ggttgccgct
    14701 ccagtggcgg agccgctgat gatctgcgcc tgggcgtgtt ccgtcgcgtt gatcacatca
    14761 tcgcctgcca ccgtgttgat ggtgacgacc ggaacggacg tatccaccgt caggttgtga
    14821 ttaaccgagg ccgggtttcc ggctttgtcg ctcaccgccg cgttcacggt gtagttgctg
    14881 gcggtcaggg tgcttaagtc ggctgacggt acgctgacgc tccagctgcc gtccgtctgt
    14941 acggtgccgg tgtagtttgt gccattgagc gtgacggtca ccgtctgccc cgcttccgcc
    15001 gtgctgctgc cggtgatggt cagagccgct ccggcttcgg cggcgttaag aatatcatcg
    15061 cccgcaatgg tattaatggt caccgttggc gcgctggcat caacgctgta agcgtgggtc
    15121 gtggtggcgc tgttgccgtt aacattgctg acgctggcct gtgcgctggc atcgccatca
    15181 cgcagagccg ccatatctgc cgccggaacc gaggtgctcc aggaaccatt cgcagccacc
    15241 gtggcgctgt aagttttgcc gccaaaggtg acggtaaccg tttgtccggc ttcaacgcca
    15301 gaggtgctgc cggagagcgt taacgccgcg cctttttcgg cagcgttgat cacatcatcg
    15361 gcggtaatgg cgttgatgct caccgccacc gccgagagat cgaccgtcac cggatgcgta
    15421 acgcttaccg gatttccggc agaggtgcta ccgctcgccg caatcgtcac cgatcccgca
    15481 ggccaggcgc tgacatccac cgccggaacg ccgacgctcc aggtgccatc cgccagcacc
    15541 gccgcaacat aggtttgccc gttaatggtc agcgtgacgt tgctgcccgc cgccagaccg
    15601 gagctgctgc cggtaatcac cagcgcctgc ccgtgctcga taatattaac cacatcatcg
    15661 cccgccacgg tgtcgacccg cagaccgggg agattcgcgt ctatggtgat ttcgcgcgtg
    15721 ccgttaccag tattgcctac gctgttcgtc accgaagccg aaatggtcag ttcgccgttg
    15781 cccaactcct gtagcgcgga ggccggaaca tcgacgctcc agcttaaatt cgcctgcacc
    15841 gtggcagtgt aagtcgcccc gccgagcgtg acggtcaccg tgtcgcctgc cgccgcaccc
    15901 gtcacctgcc cgctgatggt ctgttccacg cccgcttcgg cggcgttaat aatatcgtcc
    15961 gttgccacca cgttaatggt gacgcccggc agcgcggtat taacctgtac gttatggctg
    16021 gcggaaccgc tgttgccatc ggcgtcagtg gcggctgcgg tcaccgtgta ggtggcttca
    16081 ccgagggcgc tcacccgtga cgccggaacg gtcacgctcc agttaccgga agcatcggca
    16141 gtggtggtgt aattttgccc gttgagcgta acggtaatct gcgtgcccgc aggctggttg
    16201 ctggtgccgg taatcgccag atccgcgcct ttctccgcgg cgttgatgat gtcatcgacg
    16261 gcaatggtgt taatggcgag caccggagcg ccgagcgcca cggtgacagt gtgcgaggcc
    16321 gtgccgctgt tgcctgcgga gtcggtgacc gtagcggtaa tggtcacatc gccctgcgcc
    16381 agcgcggaaa tcacgctggc aggcacgccg atgctccagt tgccgttggc gtccagcacg
    16441 gtggtatagg tggtcgtgcc aatcgtcacg gaaaccgtat taccggtagt cgcgcccgtt
    16501 gccgagccgc tgatgatctg cgcctgtccg tgttccgtgg cgttaatgat gtcatcgccc
    16561 gccaccgtgt tgatggtgac gaccggagcg gcaaggtcca ccgtcagatt atgcgttgcc
    16621 gaagccgaat ttctggcttt gtcgctgacc gaggcgttaa cggtatacga gctggcggta
    16681 aggctagcta aatcgccggt tggtacgctg acgctccagc tcccgtctgc ctgcacattg
    16741 ccgctgtaat taacgccgtt tagcgttacg gtcaccgtct gcccggcttc cgccgtgctg
    16801 gtgccgctga tggtcagcgc cgatcccgct tcggcggcgt taagaatatc gtccgtggcg
    16861 atggtgttga tggtaacgga gggcgcagtg gcatcaacgc tgtaagcatg cgttgcctgg
    16921 gtgctgttcc ctgcgacatt gctgacgctg gcctggacgt ttgctgcgcc gtctggcaag
    16981 gttgcaacat cgacggcagg gatcgtcagc ccccaggtat tatccgcggc aaccgtggtg
    17041 gtgtagcttt tgccgccgaa gataacggtg atggtttgcc ccgcttccac gccggaggtg
    17101 gtgccggaga gctgcaaatc agagcctttc tcggcggcgt taatcacatc gtcagtggaa
    17161 agggtgttga tggtgatcgc cacggcagcg agatcgaccg tgaccggatg gctaatgctg
    17221 gttgtggttc cggcggtgtt ggtgccagag acggcaatat tgaccgtccc cgcaggccag
    17281 tttgtgacat ctgccaccgg aacaccaacg ctccagctac catccgctaa aaccgttgcg
    17341 ccgtaggtga cgctattgat aaccaccgtc aacgccgcgc ccgccgccag tccgctactg
    17401 ctaccggtga tcaccaacgc ctgagtgtgc tcgatgctgt taatcacatc atcgcctgcc
    17461 acggtatcga cgcgcagacc tggcaggttg gcatctatca cgatatcgtg cgtcgcggtg
    17521 ccggtattat ttgcggaatt ggtcaacgag gcgctgatgg tcagttcacc gttacccagc
    17581 gcctgcaaaa cgttcgccgg tacgctgacg ctccagctta aatctgactg cacggtggcg
    17641 ttatattgat tgccgccaat ggtgacaatc accgtattcc cggcaaccgc cgttcccgtg
    17701 acctgaccgc tgatggtctg cgccacaacg atttcagcgg cgttgataat gtcatcgccg
    17761 gagacggtgt taatggtcag cgtcgggccg ctaagatcaa cggtcacatc gtgagtcgtg
    17821 ctgtcgctgt tgcccgcgcg atcgctcagg ctggcggtga tggtctggct accggtcgcc
    17881 agcgccgtga catccgccgc cggaatgccc acgctccagt taccggaggc atccagcgtg
    17941 gtggtgtagt ttttaccgtt cagcgtgacg gttaccacat cacccgccgc cgcgcccgta
    18001 ctggtgccgc tgaccaccaa cgcctgacca tgttcgatgg cgttgataat gtcatcgccc
    18061 gcgacggtgt taatggtgag atccggcgca gtgatatcta ccaccagcgc gtggtcggcg
    18121 cttgccgggt tacccgcttt gtcgctcacc gtggcggtca cggtgtaact gctggcggtt
    18181 aatccgctta aatccgctgc cggaacattc acgctccagg tgccatccgc cagaacggtg
    18241 gtctggtacg tgttgttatt gagcgtgacg gtgagcgtct gcccggcctg cgccgtggta
    18301 gtgccgctga tggtgatccc cgcgccagct tcgctcacgt taaggatatc gtcgctggcg
    18361 atggtgttga tggtcacaag cggcgcggtg gcatcaacgc tatacgcgcg atcggcctgg
    18421 gcgctgttgc cgttaatatt actgacactc gcctgcacgt tgcccgcgcc gtccggtagc
    18481 gcagcgagat cggcaggcgg cacattcacc gtccagctac cgttagcttc cactgtggtg
    18541 gtgtaatttt tgccgccaaa ggtgacggtc accgtctgcc cggcttcgac accgctggtg
    18601 ctgccgctta cgaccagcgt ttcgcctttt tcgacggcgt tgatcacatc atcaccagag
    18661 agagtgttaa tggtgatggc gaccccagcc agatcgacgg taaccggatg ggtaatggtg
    18721 gaggttgttc cggcactgtt ggtgccgctt accgtgatat tcaccgtacc cgctggccag
    18781 ttgctgacgt ctaccgccgg aacaccgagg ctccacgtgc cgtcggctaa taccgtcgcg
    18841 ccataagtaa cgttgttaat ctcgaccgtt aacgccgttc ccgccgttaa tccgctgctg
    18901 ctgccggtga tcaccagcgc ctgattgtgc tcaatgctgt taataacatc atcgcccgcc
    18961 acggtatcga cccgcaggcc aggcagattg gcgtcgatcg tgatatcgcg cgagccgctg
    19021 ccggtgttgc cgacgccatt ggtgaccgag gcattaaccg tcaggtcgcc gttgcccagc
    19081 gcctgaatat ccgccgccgg aacgtccaca ctccagctta aattagactg caccgtggcg
    19141 gtgtaggtgt tgccgcccag cgtaacggta accgtatccc cctgcgctgc gcccgtcacc
    19201 tgcccgctga tggtttgcgc atttcccgat tcggcagcgt taataatatc gtcagtcgcc
    19261 accgcgttaa tggtaacggc aggcaaggcg ctgttgacca gcacattatg actggcggaa
    19321 ttactgttgc ctgccgtatc agtgacgttt gccgttaccg tgtagttggc ttcacccaat
    19381 gcgctaaccg ctgacgctgg caccgtggcg ctccagttgc cgttgctatc ggtagtagcg
    19441 gtgtaatttt gcccgttcag cgtcaccgta atggtggtgc ccgcaggctg attactggtg
    19501 ccggtgattt gcaggtccgc gcctttttcc gtggctttaa tcacgtcatc ggtggcgatg
    19561 gtgttgatac caatggttgg cgcggcgaga ttcacggtga ccgtgcggct ggcgtcatcg
    19621 ctgttgcctg ccgcgtcggt aattgccgca gtgatagttt gcgggccgct gccaagcgca
    19681 gtgacgtcag ccgccggaac gccgacgctc cagttgccgg aagcgtccag catggtggtg
    19741 taggttttac tgtttagtgt gacggtgatg acatcacccg cttcgccgcc agtgctggag
    19801 ccggagatca ccagcgcctg tccgtgttcg gcggcgttaa taatgtcatc gccggagacg
    19861 gtgttgatgg tcagcaccgg cacggtgaga tccaccgcca gcccgtggtt agcggacgcc
    19921 gggttacccg ctttatcgct taccgaggca ctaacggtgt actggctggc ggtcagattg
    19981 ctgagatccg ccgtcggtaa gctgacgctc cagctgccgt ccgcctggac ggagccgctg
    20041 taagtcacac cattaagcgt gacggttacc gtctgccccg cttcggcggt gctgctaccg
    20101 ctgatggtta acggattgcc cgcctcggca gcgttcagaa tatcgtcggt ggcgatggtg
    20161 ttaatggcaa gcgtcggggc cgtggcatcg acgctgtagg cgtgggttgc cgaagccgtg
    20221 ttgccgttaa tagtgctgac gctggcctgc acggtggcgt cgccgtcgcg taacacgctg
    20281 agatcggcgg cgggtacggt ggttgtccag ctaccatcgc ccgctacggt agcggtgtag
    20341 gttttcccgc caaaggtaac ggtgaccgtt tgccccactt ccacgccgga ggtgctgccg
    20401 gaaagggtta aatctgcccc tttttcggcg gcgttaatca catcgtcgcc ggaaacggtg
    20461 ttaatggaaa tcgccaccgc cgccagatcg acggtgaccg gatgggtgat ggtggatgtt
    20521 gttccggctg tgttagtgcc acttaccgtg atattcaccg tacccgcagg ccagttactc
    20581 acgtctgccg ccggaacacc aacgctccat gtgccatcgg ctaatactgt tgcagcgtaa
    20641 gtgaccgtgt taatcaccac cgtcagcgcc gcgcccgccg ccagcccgct gctgctacca
    20701 gttatcacca gcgcctgagc gtgctcgatg ctattgacca catcatcgcc cgccacggtg
    20761 tcaacgcgca gacctggcag gttggcgtcg atagtaatat ctcgcgaacc gctgccagta
    20821 ttgccaacgc cgttggtcac cgaagcgtta accgtcagat tgccattgcc gatggcctga
    20881 atatccgccg ccggaacgtc cacgctccag cttaaattcc cctgcacggt agcggtgtaa
    20941 gttttcccgc caagcgttac ggtaaccgta tcgcccgccg ccgcccccgt cacctggccg
    21001 ctgatggttt gcgcattgcc cgattcggcg gcgttaataa tatcgtcggt cgcaactggg
    21061 ttaatggtga cgccaggcag cgcggtattg acctgcacgt tatggctggc ggaattgctg
    21121 ttgcctgcgc tgtcggtgac gttcgccgtc accgtgtagc tggcttcgcc cagcgcacct
    21181 actgcggaag ctggcaccgt agtgctccag ttgcctgcag cgtccgtcgt cgcggtgtaa
    21241 ttttgcccgt taagcgccag cgtaatcgtg gtgcctgctg gctgattact ggtgccggaa
    21301 atttgcagat ccgcgccttt ttcgctggcg ttaattatgt catcggtggc gatggtgttg
    21361 atgtcaatgg ttggtgcggt gaggttaacg gtgaccgtgt gtgtctaatt gtcgctgttg
    21421 cctgccgcat cggtaaccgt ggcggtgaca gtttgcgggc cgctgccaag cgccgtgaca
    21481 tccgccgccg gaacgccgac gctccagttg ccggaggcgt ccagggtggt ggtgtaggtt
    21541 ttactgttta gcgtgacggt gacgacatcc cccgcttctc cgccggtgct ggaaccggaa
    21601 atcaccagcg cctgcccgtg ttcggtggcg ttaataatgt catcgcccgc aatggtgttg
    21661 atggtcagca ccggaacggt gagatccacc gccagattat gcgtggtgct cgccggattg
    21721 cccgctttgt cattgaccgt ggcggtcagg gtgtggctgc catcggtcaa tgcctcaaga
    21781 tctgccgccg gaatattgat gctccagctg ccgtccgcct gcaccgttgc ctgataggtc
    21841 tgcacggtcg ggctgttaag cgttatcgta acaatctgcc ccgcttcggc ggtggtactg
    21901 ccgctcaccg tcacgcccgc atcggcttcg ctggcgttga cgatattgtc gctggcgacg
    21961 gtattgataa tgatggttgg cgcgctgctg tcgacgctgt agttgtgcac cgccgaggcg
    22021 ctattgccgt tgatattgct gacgctggcc agtgcggagg cgctgccctc gggtaatgac
    22081 gccagatcgg cggcgggtac ggtggcagtc cagctaccat cgctcgctac gctggcagtg
    22141 taatttttcc cgccaaaggt gacggtgacg gtttgaccgg gttctacgtt agtggtggtg
    22201 ccggaaaggg ttaaatcagc gcctttttct gcggcgttaa tcacatcgtc cgtggcgatg
    22261 gtgttgatgg tgatcgctgc cggagtgaga tccaccgtca ccggatgcgt aatgctcacc
    22321 gagtttccgg cgctactttc ccctgaaacg gcaatattaa ccgtccccgc aggccaggcg
    22381 ctaacctgcg ccgccgtgac gcccacgctc cagctaccat cggcctgcac cgcagtggtg
    22441 tattccacat tattaatcgt aacggtaagc ggcgtgcttt cagccagccc cgagctgctg
    22501 ccggtgacca ccagcgcctg cccgtgctcg atgatattga ccacatcatc gcccgccacc
    22561 gtatcgaccc gcaggccggg cagattggcg tcgatggtga tatcccgcgt gccgctgccg
    22621 gtgttgccgt tttgattggt gactgaggca ttaaccgtta aatcgccatt tcccagcgcc
    22681 tgaatatctg ccgctggaac gtccacgctc caggtgagat tgctgcccac cgtcgccgta
    22741 taagtattac cacccaacgt aatagttacc gtgtcgccat cttccgcgcc agtcacctgc
    22801 ccgctgatgg tttgcgccac gcccgcttcg gcggcgttaa taatatcgtc ggttgccacc
    22861 ggattaatgg tcacaccggg cagcgcgctg tcgaccagca cgttatggct ggcagtggcg
    22921 ctgttgccga tatcgctggt caccgccgcc gttaccgtat agttggcctg gcctaatgct
    22981 gtaaccgccg atgccggaac ggtgacgctc cagttgccgg aggcgtcggt cgtggtggtg
    23041 taattttgcc cgttcagcgt cacggtgatg gcggtgttaa caggctgatc gctggtgccg
    23101 gtaatctgga gatcggcccc tttttcgctg gcgttaatca catcatcgcc cgcaatgctg
    23161 ttaatgccga taaggggcgc ggcggtattg accgtgacgg tcaatgactg gctgcccgtg
    23221 tttccggctt tgtcggttac cgagacgctg acaggataac tgccgtccgc cagcccactg
    23281 accaccgagg ccggaacgcc cagactccag ttgccggaac catccaccac cgtggtgtaa
    23341 tccacattat tcagcgtcac ggtgacgata tcgcccgcct gagcgccagt aaccgtgccg
    23401 ctgatgattt gcgcctgagc atgttccggc gtgttgatga cgttatcggt cgccaccgga
    23461 ttaatggtta ccaccggcgg cgtggcatcc accagcgcca cgcgtgaaac gctgctcgga
    23521 ttaccggcga catcgttcac gctggcggtg accgtagcgt tgccgtcagc cagtgcgccc
    23581 acgttggcgg cgggcacagt cacgctccag gtgccgtctg gctgcacgac gccctgatac
    23641 gtctgcccat ttaacgtaac ggtcaccgtc tgccccggct gagcgtcggt ggtgccggtg
    23701 atgggcagat cctgcccgct ttcggcggcg ttaatgatgt tatcagcggt cagtggatca
    23761 atgcttaagg ccggggcctg gctatcgacg gtaatggtgc gggaagtgtt gccggtattg
    23821 ccgctgagat ccgtcacaga agcggtaatc gccaccgcgc cgtcaggcag atttcccatc
    23881 gcggcggcag gtaccgtcag actccaggag ccatcggcct gcacggtagt ggtaaaactc
    23941 tgtccagcca gggttactgt gacggtttgt cctgtctcag cctgttgagt gctaccggta
    24001 agggtcagtg gcgcaccttt ttctgcgcca ctgataatgt catcgccgga gacggtgtta
    24061 atggtgatca acggagaaac accgaccagc gtgattgctc gagacgccgc gccagtattt
    24121 cctgcagcat ctgtcacggt gacgaaaatc gtattggctc cgcgatccag cgtgcgggtt
    24181 accgctgggt ccagcgccac attccagctg ccatctgcca gcacgatgcc cgtcaggaca
    24241 tgagtgccca atttcaccgt taccgtatcg cctggagaag caccgctgac ttgtccggag
    24301 atgatctgcg cgacggcctg ctcggcatta ttaagaatat cgtcgcccgc cacggtatta
    24361 acactgacca cgggtgctga ggtgtctacc gtgaaattcg cgctgtggct ggtggtgttc
    24421 cctgcccgat cgctgacact ggcattgaca gcgtaattac cctccgccag tgcctggact
    24481 tccgtggcag gcagcgtcac ttgccaggtt ccgtcgctac cgacggttgc tgtatggctt
    24541 ttcccgttga cggtcagtgt tacggtttgc cccgcttctg cattcgacgt gccgctcagt
    24601 accagcgcga cgttatgttc cgcagcactg ataatgttgt cctgagcgat ggtgttgatg
    24661 gcaagcgttg gcggttgggt atcgaccgtt agtagctgcg cgccggtgac cgtattgcct
    24721 gtggcgtctt tcccactgac cagcaccgcc tgattccctt cgcctaacgc ctgtgcatcg
    24781 gcggtcggca cctgcacgct ccaggcaccg ttagcgccta cgctggtcgt gtaggttttg
    24841 ccgtttagcg taacggtgag ttctgttccc tgcgccagat ggctactggt gccagaaagc
    24901 gtaaacccgg cggcctgttc gctggcgtta ataatgttat cgcctgcggt ggtggcaacc
    24961 tgcacgctgg cctgactatt gtcgatggtt aacgttatcg aaccggcgtc agtgatggta
    25021 ctgccgacaa tttgcaccac tgaccaggta tgttcacctg cactttgcgt gggtaacgag
    25081 acactccagt gaccgttgtt gtctacctga gtactggcaa tagtattgcc gttactgtct
    25141 ttaatctgaa tagtcgcccc cgcctgcccg ctgccgctaa aggttggcgt ggtgtcatcg
    25201 gtgatgtcat tggtggctag aatgccgcgc tgatcgccct gattatcggt aactaaaaat
    25261 gatggtgtgg cgttgccagg ctcagcaggt ggcgtagggt tattaatcac ttctgttttc
    25321 gagtcgccat cgccaccgct tgccagcagt gcaccaagcg cgccaccacc taccgccgcc
    25381 cccgccagcc aaccccacgg gaaggcgctg gtctgagcaa cggtatcaag aaatggcgca
    25441 atgctttcaa tcgcagtggt ctgggcagtc agttctacgg gggctaatcc acccgcagca
    25501 gtatcggcaa aggtgatatg ggttagctgt tgaccatcgg caaacaccag ttccgattgt
    25561 tcagctgtat tcgccgcttg caggaaataa ccgttgcagc ggatcaccgt gccgtcctgc
    25621 atatagatca gcaggtcatt tccttcacgg acgtaacgcg caacggcaga ggcctgagca
    25681 tgaacctgca ccacggaagg ctcataaata acaaccgtga cgtcgccagg attaatcagc
    25741 gtttgtttga cggatgtttt acgcgaaata acatcaataa tcaggctcat aatggcattc
    25801 ctgataaatg gcgcacatcc ttagcgagtc catttatcta aataagacaa aggtaatcac
    25861 ttaacaacgg cccactatta ataatggcag gattatttca agtcgggtaa gtcgttgtat
    25921 aagccctgtt taattcccgc taatggaata aggttattca ccgcagcggc ataatttacg
    25981 gcggcgaccc agccatcata attggcattt atttcagccg attgcgcctg aaagacatct
    26041 tgttcgacgg ttaatagatc attcagactg cgtttaccca atttatattc attttggtac
    26101 acatctcgcg ttttgtgcgc actttcggat tgcgctaaac cggcttcttc acgaccccgt
    26161 gcgccggtcc agttcgcata tgccacagac gctcgttgca gcacatccag ttttgcctgt
    26221 tcgacctgcg aggcagagat tttttgttgc ccttcggcct gttgcacctg ggcagaaacc
    26281 gcgccgccct gatacagcgg cgcgttaacg ttcagttgta gctgatcatc ccaatacgag
    26341 cggtcgctgg tctggtagcg cgttttaccc ccctgaatac tgagcgttgg ccagtattgc
    26401 gctttcgttt tttccacgcc gtactgtgct gactggcgta agttttctgc cgccagcacc
    26461 agcgggatgg actggtaatc aatattcttc agcgataccg gctgctcggc taattcagca
    26521 ggtggcgcgg ctatcgcctc cggctgtacg ccagtgagca ccgccagttg cgctttggcg
    26581 ctcgccatct gcgcctgata ttgctccagc gttgagcgca ttccggcaat acgcgtctgg
    26641 gcctgtaatt catccgacga tgagttcagg cctgcgttag cgcgcaatgc cgccatgttg
    26701 tagacgtttt ccagcgagtg aatattgcgt tgagccgcat cgcacaaagc ctgataacga
    26761 ctgacttcca tgtaggcagt cgccgttttc tctgcaacat cggttaaggt tgccatcaat
    26821 ttaaagcggt agctgtcacg ggcggcagtt tgcagattga tatcgttgtt agttttacca
    26881 aagtcataca ccagttgtgt cagggttatg ccatacgaaa cgttgttatc aagcctgccg
    26941 ctggagtccg tttgtcggga cggccccgcg ttgccggtta atccaacgtg cggataccag
    27001 gcacttttgg cttcatcaat ttgcgcttcg ccaataccta tctgcgccgc ctgttgagtg
    27061 acaccgggat ttcgcgcaaa ggcgcgtaat atactttcct gtaaagataa ttgggcaact
    27121 ggcgttaccg ctggggcatt cgcccattgt ggcagcggtg cagcaattgc agcttctccc
    27181 atcaccataa aacttataaa aaaaataagt ccattatttt tacggttttt aaactggaat
    27241 cgcgccatag ataagtcact tccttccagg tcattat
//
LOCUS       DAZKIP010000053        27149 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZKIP010000053 DAZKIP010000000
VERSION     DAZKIP010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.053"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(246..464)
                     /gene="infA"
                     /locus_tag="ACHOUK_002498"
     CDS             complement(246..464)
                     /gene="infA"
                     /locus_tag="ACHOUK_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFP9897928.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(749..1453)
                     /gene="aat"
                     /locus_tag="ACHOUK_002499"
     CDS             complement(749..1453)
                     /gene="aat"
                     /locus_tag="ACHOUK_002499"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFP9897929.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRNYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(1495..3216)
                     /gene="cydC"
                     /locus_tag="ACHOUK_002500"
     CDS             complement(1495..3216)
                     /gene="cydC"
                     /locus_tag="ACHOUK_002500"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFP9897930.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDS
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAVIL
                     RRVGLEKQLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(3217..4983)
                     /gene="cydD"
                     /locus_tag="ACHOUK_002501"
     CDS             complement(3217..4983)
                     /gene="cydD"
                     /locus_tag="ACHOUK_002501"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFP9897931.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGETELASTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(5106..6071)
                     /gene="trxB"
                     /locus_tag="ACHOUK_002502"
     CDS             complement(5106..6071)
                     /gene="trxB"
                     /locus_tag="ACHOUK_002502"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFP9897932.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            6616..7110
                     /gene="lrp"
                     /locus_tag="ACHOUK_002503"
     CDS             6616..7110
                     /gene="lrp"
                     /locus_tag="ACHOUK_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFP9897933.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            7245..11273
                     /gene="ftsK"
                     /locus_tag="ACHOUK_002504"
     CDS             7245..11273
                     /gene="ftsK"
                     /locus_tag="ACHOUK_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFP9897934.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDENIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLSPPPFD"
     gene            11428..12039
                     /gene="lolA"
                     /locus_tag="ACHOUK_002505"
     CDS             11428..12039
                     /gene="lolA"
                     /locus_tag="ACHOUK_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFP9897935.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            12050..13393
                     /gene="rarA"
                     /locus_tag="ACHOUK_002506"
                     /gene_synonym="mgsA"
     CDS             12050..13393
                     /gene="rarA"
                     /locus_tag="ACHOUK_002506"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFP9897936.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            13484..14776
                     /gene="serS"
                     /locus_tag="ACHOUK_002507"
     CDS             13484..14776
                     /gene="serS"
                     /locus_tag="ACHOUK_002507"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFP9897937.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            15015..17459
                     /gene="dmsA"
                     /locus_tag="ACHOUK_002508"
     CDS             15015..17459
                     /gene="dmsA"
                     /locus_tag="ACHOUK_002508"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFP9897938.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSGEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGIHNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            17470..18087
                     /gene="dmsB"
                     /locus_tag="ACHOUK_002509"
     CDS             17470..18087
                     /gene="dmsB"
                     /locus_tag="ACHOUK_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFP9897939.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLMPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            18089..18952
                     /gene="dmsC"
                     /locus_tag="ACHOUK_002510"
     CDS             18089..18952
                     /gene="dmsC"
                     /locus_tag="ACHOUK_002510"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFP9897940.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(18988..19614)
                     /gene="ycaC"
                     /locus_tag="ACHOUK_002511"
     CDS             complement(18988..19614)
                     /gene="ycaC"
                     /locus_tag="ACHOUK_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFP9897941.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            19929..21077
                     /gene="ycaD"
                     /locus_tag="ACHOUK_002512"
     CDS             19929..21077
                     /gene="ycaD"
                     /locus_tag="ACHOUK_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9897942.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            21287..22717
                     /gene="ycaM"
                     /locus_tag="ACHOUK_002513"
     CDS             21287..22717
                     /gene="ycaM"
                     /locus_tag="ACHOUK_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP9897943.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYTPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(22718..22828)
                     /locus_tag="ACHOUK_002514"
                     /pseudo
     CDS             complement(22718..22828)
                     /locus_tag="ACHOUK_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(22927..23667)
                     /gene="pflA"
                     /locus_tag="ACHOUK_002515"
     CDS             complement(22927..23667)
                     /gene="pflA"
                     /locus_tag="ACHOUK_002515"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFP9897944.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(23859..26141)
                     /gene="pflB"
                     /locus_tag="ACHOUK_002516"
     CDS             complement(23859..26141)
                     /gene="pflB"
                     /locus_tag="ACHOUK_002516"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFP9897945.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(26196..27053)
                     /gene="focA"
                     /locus_tag="ACHOUK_002517"
     CDS             complement(26196..27053)
                     /gene="focA"
                     /locus_tag="ACHOUK_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFP9897946.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         6669 a         6737 c         7137 g         6606 t
ORIGIN      
        1 ttgctgccaa acctcatggg tggcaacggg gcgctactat agggagttgg agtaaaacgg
       61 tcaagaagaa ttttaatgat aattattgtt tgctcatact gtaaacaact tgtgcagtat
      121 atctacatcg agacaagtta tggacttata cttccaaagt acttcatgca tatcacaaaa
      181 taaaaaggcc ggttaaaccg accttttact cgttctttct cttcgcccat caggcggtaa
      241 aacaatcagc gactacggaa gacaatgcgg cctttgctca ggtcgtacgg ggtcagttca
      301 acagtcactt tgtcgcccgt caggatgcgg atgtagtttt tgcgcatttt accggagatg
      361 tgtgcagtaa ccacgtgacc gttttctaac tctacgcgga acatggtatt aggcaacgtt
      421 tcaagaacgg taccttgcat ttcaatattg tcttctttgg ccatctaatc ctctggggta
      481 tcactaccgt aatttgaacc ggcaagataa tgccgaagtt ctgtaaataa gtaaagattt
      541 gcgcgctaaa tcgcaacaaa caggttcggc acattactcc gaaaacacac ggctaagccg
      601 caccaaaagc gcaacgtata agggagcggt gagataaacg atgggcgtta cctgacgcga
      661 aaaattcctt atcggcagcg gggtaatgag cgtaaccaac tctgcgaccg caattataac
      721 actctgggga gaaatgtgcc gaaaacattc attcttgtgg tgaaaacaag catcgtggta
      781 cccagaaatt attcggcaat cgtccgaggc gcatttgatt gagataatta aggtaattcc
      841 ggcggggaat ttcgcaggca ccaagcgatg ctgtgtgatc gttaaggacc tggcagtcga
      901 taagcttacc gccatgaccg ataaattcct cacagaatac cagaagcgcc gttttagacg
      961 cattttccat ccggctgaac atggactcgc cacaaaatag cgttccctgg gccacgccgt
     1021 acataccgcc gacaagctca tcttcacgcc agacttcaat ggagtgggca tgaccgagtt
     1081 cgtgaaggcg atggtaggct tcgaccacgc cacgcgtgat ccaggttcct tcttcgcgat
     1141 cgctggcaca gccttcaatg acctgaccaa aagcgtaatt catcgtgaca cgatagggcg
     1201 agcgtttatg aaatcgcttc atactacggc tgatatgcag tgattctggc catagcaccg
     1261 cgcggggatc gggcgaccac cagaggatgg ggtcgcctgg agaaaaccac ggaaaaatac
     1321 cacgctggta agccattaac aggcgcgcag ggctaagatc gcccccaagt gccagcaggc
     1381 cgttaggctc acgtaatgcg ccttccgggg aagggaaggc tattgaatgg cgagaaagct
     1441 gaaccaggcg catgaccgca aaactccacg caagtcggat cgttcaataa tagcttacaa
     1501 accctgcttg aactggtaat aacgcccctg tctggcaagc agttctgcgt gagtaccttg
     1561 ctcaataatt tgcccgttgt ccatcactat tatttgttgg aaacgagaga gtccgcgaag
     1621 tcgatgggtg accattaaca ccgttttctc acgcatcatt tctgcaagca attcaaggat
     1681 ctggctttcg gttgtggcat ctaagccttc ggtaggttca tccagcaaca ccagtggcgc
     1741 atcatgtaac agcgcacggg cgatagccag acggcgcagt tcaccaccgg agagctggcg
     1801 tccgccttca cctaaccaac tgttgagacc tgcatcctcg agctgcttct ccagaccaac
     1861 gcgacgtaag atcaccgcca gagcctcatc actactgcca ggcgaggcga gtaacagatt
     1921 atcacgcagc gtggcgctaa acagatgcac tcgctgagga acaacgctga tggtctgtcg
     1981 tagagccgct tcattcaggc tggctatggg gctatcgtta agcaaaatct cgccctgttg
     2041 cgggtcccat gcgcgggtca gcagttgtaa cagtgttgat ttgccgcatc cggttcgccc
     2101 gagaatcgct atatgttccc cggcgtttac ctgaagagaa atccctttaa gtgcctgttg
     2161 ggattgctcc ggataagtga actgaacatc ccgtaatgtc agcgaaacgc gatcggcaac
     2221 acgagtttgg gtatcaggga aggtgacctc cggtttttga tccgttaagt cagagatacg
     2281 tacggcagag gcaatgactt gccccagatg ctgaaatgca cccgttactg gtgccaatgc
     2341 ttcaaacgcg gctaacgcgc aaaagacaaa cagggcaatt aacgcgccgg gttgagcatt
     2401 gccgccaacg ccgccagacg ccatccacag catcaggatc accgctaacg cgccaatgag
     2461 cagcattatc gcttgcgaca atgcggtcag ttcagattga cggcgttgcg cttccagcca
     2521 ttgaatttct gtattctcta gttgcgtgcg ataacgatcg ctggcaccaa aaatggtcag
     2581 ctcagcttgc ccttgcagcc aggccgtcag ttgttggcga tactgtccgc gaagatgagt
     2641 cagattttgc ccggtgcttt ttcccgcacg ataaaacagc ggtggcatca ggaaaagcgt
     2701 cagtaacata atgccgccca gcgtaaaggc gagggtggaa tcaaggaaac ttaacccgat
     2761 tgtcaccacc ataatcacca caaaagcgcc caccagcggc gagataacgc gcaggtaaag
     2821 atgatcgagc gtatcaacat ccgccaccac gcggttgagc aattcgccct gacgatagcg
     2881 cgccagtccg gcaggggaga ggggcaacaa tttgctgaag gtgtaaatgc gcagatgctg
     2941 caacacgcgg aaagtcgcgt cgtgacttac cagacgttca aaataacgcc cggcagtacg
     3001 ggtgattgct gcaccacgca cgcccgcagc gggtagcata tagttgaagc tgtacagtcc
     3061 ggcaaccccc gcaaccgctg aggccgagag gaaccagccg gaaagtgtca acagaccgat
     3121 actggcgagc agcgtcacaa ttgccagcac aataccaaga cttaacatcc atttatgacg
     3181 tttatacagt gccagatagg gtagcaaagc gcgcatttaa atctcctcct gacgatgggc
     3241 cagtaatgtg gcgaatgggc caccagccac acttaattcc gcgtaacgtc cttgctcaat
     3301 aatccggcca tcctgcataa cccaaatgac atcccagtca gcaagatctt ctaactggtg
     3361 ggtgaccatt aacgttgtct ggcgcagaga ggcggcattc agcgcctcca ttacgcgctg
     3421 ttcactgtga gcatcaaggc tggcagcggg ttcatccaac agtaatagcg aacagggatt
     3481 tagtaacgca cgggccaccg ccacgcgctg cgcctgcccc acggaaaggc gggcagcctg
     3541 gtcgccaaca ggcgtatcaa cgccttgtgg caggagcggt agaaactcgc tgacccaggc
     3601 gttatccagc gctgcttgta attcttgttc gctggcatca ggtcgcgcca gtagtacgtt
     3661 atcccgtaat gttgctgccg gtaattgtgg gttttgacca acccaggaga gatgtttacg
     3721 ccatgattct ggtgataaat cgcgtaattc tatcccgttg attcgtaatg atccctgata
     3781 tgagagaaaa ccagaaagcg cgttcagcag tgagctttta cctgaaccgc tgcgaccaac
     3841 caacactgca cgttggcctg ctggcaaagt aaagttcagc ggtccggcca gcgttttacc
     3901 ttccggcgac gtgataaaca gatcctcggc ctcaatggtc agcggatcgg ttgatgctaa
     3961 ttccgtctca ccgcgctgcg gatgggcgag cggggtttcc ataaacgttt tcagactgtc
     4021 agctgcacca acagcctggg ctttagcatg ataaaacgta ccgagatcgc gtaatggctg
     4081 gaaaaactct ggcgcaagga tcagggccag aaaacccgca gccagcgtca cgccagtatc
     4141 gtagtgacca aaatccagct cgccgagata ggaaaaacca aagtagaccg ccaccagagc
     4201 aattgacagc gaggtaaaaa attcgagaat gccggaggat aaaaacgcca gccgtagcac
     4261 ttccattgtc cgttggcgga aatcttccga agcagaacga atactttcaa tttcagcttc
     4321 accacgacca aaaatacgca atgtttccat gccgcgcagg cgatcgagga aatgcccact
     4381 taagcgagca agagcgagaa agttacgtcg gttagcatcg gcagccccca tcccaaccag
     4441 cgccataaac aacggaatta acggtgcagt gcccagcaga atgagcgccg cagcccagtt
     4501 agaggggaag atcgccacca caatcagcaa cggcaccgac actgccagcg ccatttgcgg
     4561 caggtagcgt gcatagtaat catgcatatc gtcaatttgc tcgagtacca gcgtcgccca
     4621 gctccccgca ggtttaccct gaatccacgc tggccctgct tgttgcagac ggtcgagaac
     4681 ctgacggcgg atggcaaagc ggatatgctg cccggcgtga taacccaccc gttcgcgtaa
     4741 ccagaccacc catgcgcgca gtacaaaggt cagaaccagt aacgtaaagg gaagcagcag
     4801 ggcttcacgg ggaatattct ccataatcat atgttgcaga attcgcgcca tgaaccatgc
     4861 ctgggcaatg atcaatatgc cgctcacaaa gcccagcaga cgagaaatat tcagccaacg
     4921 ttgggagatg acgctttgct gttttaacca gcgggttaac tctttttgac gagatttatt
     4981 cattgcacgc ttagcaggtg agttatcaga attatttgca gagcaatgtt acaacgggga
     5041 aaaaataaag gcgacccata gtcgcatggt gtcgccttct ttacttttgt tactgatttg
     5101 taaaattatt ttgcgtcagc taaaccatcg aggtagcgtt ccgcatcaag tgctgccatg
     5161 cagcctgtac cagcagatgt aatggcctgg cgataaatgt gatccatcac gtcgcctgcg
     5221 gcaaagacgc caggaatgct ggtctgggtg gcattaccat gaatacccga ctgcactttg
     5281 atgtagccgt tttccagttc cagctgccct tcgaaaatgg cagtattcgg gctgtgaccg
     5341 atagcaacaa acagaccggc aacgtcgagt gactcgatgt tatcgctgtt ttgcgtatcg
     5401 cgcagacgaa cgccagtgac acccatttga tcgccggtca cttcttccag cgtacggttg
     5461 gtgtgcagaa tgatgttgcc gttctccact ttatccatca gacgcttaat gaggattttt
     5521 tccgcgcgga aaccgtcacg gcggtgaatc agatgcactt ccgaagcgat gttagacaga
     5581 tacagcgcct cttcaaccgc ggtattgccg ccgccgatga ccgcaacttt ctggttgcga
     5641 tagaagaaac cgtcgcaggt tgcacaagca gaaactccac ggcctttaaa ggcttcttca
     5701 gagggcaggc cgagatagcg tgcagaagct ccggtggcaa taatcagcgc gtcgcaagtg
     5761 tattcgccgt tatcgccatt cagacggaac ggacggtttt gcagatccac cttgttgata
     5821 tgatcaaaaa tgatctcagt ttcaaacttg gtggcatgtt cgtgcatgcg ctccattaat
     5881 aacggaccgg tcagatcgtt tggatcgcca ggccagtttt ccacttccgt ggtggtggtc
     5941 agttggccgc ctttttccat gccggtaatc agcacaggtt gcaggttggc gcgcgccgca
     6001 tagacagcag cggtgtatcc cgccgggcct gaacccagga taagcagttt actgtgtttg
     6061 gtcgtgccca tgagatcccc atagttgttg gcagacaatg ggcaggattg tagggaattt
     6121 acagacgtaa aaaaagagta tgacgatttt gttaacaatt tgtgcaatcg gcagcatcga
     6181 taagcaggtc aaattctccc gtcattatca cctctgctac ttaaatttcc cgctttataa
     6241 gccgattaaa tgatgaataa acgcccctgt taatgaatat ctggcatgtt gtactaaaaa
     6301 tcgatgtttt gctttgacaa tcccctggtg ttttgcgaaa acattcgagg aagaaaaaaa
     6361 acagtattct tatatgcgca taaccatgca tgtaaatacc atgtttaccg tgctagtgaa
     6421 atctacgtat ggcgtggaca gacgccattc gtgatgtcga tagctgccac aaggcaacgg
     6481 tcttctcacc gtagacccag gcattgcgcg ccgtgaatct tcatgatttc ggtctatcgt
     6541 gacgggtagc gactctgaac agtgatgttt cagggtcaga caggagtagg gaaggaatac
     6601 agagagacaa taataatggt agatagcaag aagcgccctg gcaaagatct cgaccgtatc
     6661 gatcgtaaca ttcttaatga gttgcaaaag gatgggcgta tttctaacgt cgagctttct
     6721 aaacgtgtgg gactttcccc aacgccgtgc cttgagcgtg tgcgtcggct ggaaagacaa
     6781 gggtttattc agggctatac ggcgctgctt aacccccatt atctggatgc atcacttctg
     6841 gtattcgttg agattactct gaatcgtggc gcaccggatg tgtttgaaca attcaatacc
     6901 gctgtacaaa aacttgaaga aattcaggag tgtcatttag tatctggtga tttcgactac
     6961 ctgttgaaaa cacgcgtgcc ggatatgtca gcctaccgta agttgctggg ggaaaccctg
     7021 ctgcgtctgc ctggcgtcaa tgacacacgg acatacgttg ttatggaaga agtcaagcag
     7081 agtaatcgtc tggttattaa gacgcgctaa cacggaacag gtgcaaaatc ggcgtatttt
     7141 gattacactc ctgttaatcc atacagcaac agtactgggg taacctggta ctgttgtccg
     7201 ttttagcatc gggcaggaaa agcctgtaac ctggagagcc tttcttgagc caggaataca
     7261 ctgaagacaa agaagtcaca ttgacaaagt taagtagcgg ccgccgcctt ctggaggcgt
     7321 tgctgatcct tattgtcctg tttgccgtct ggttgatggc tgccttacta agctttaacc
     7381 cttcggaccc cagctggtcg caaacggcct ggcatgaacc tatccataat ttaggtggga
     7441 tgcccggtgc gtggttggca gatacgctgt tctttatttt tggcgtgatg gcttacacca
     7501 ttcccgtcat tattgtcggc ggttgttggt ttgcctggcg tcatcagtcc agcgacgaaa
     7561 acattgatta ttttgccgtt tcgctacgca tcattggcgt tttggcgctc atccttacct
     7621 cctgtggtct ggcggcaatc aacgctgacg atatctggta ttttgcctcc ggtggcgtca
     7681 ttggcagctt actaagcact acgctacaac cactgctaca cagtagcggg ggaactattg
     7741 cgctgctctg cgtttgggca gcgggcctga cgttgttcac cggttggtca tgggtgacca
     7801 ttgctgaaaa actcggcggc tggattttaa acattctcac cttcgccagt aatcgtaccc
     7861 gtcgcgatga tacctgggtc gatgaagatg agtatgaaga cgacgaagag tatgaagatg
     7921 aaaaccacgg caaacagcat gaatcacgcc gtgcccgtat tcttcgcggc gcgctagcgc
     7981 gtcgtaaacg gttggcggaa aaattcatta atccgatggg gcggcaaaca gacgctgcgt
     8041 tgttctccgg taagcggatg gatgatgacg aagagattac ctacactgca cgcggtgtgg
     8101 ctgccgaccc ggacgacgtc ctattttcgg gcaatcgtgc aacgcagcca gaatatgacg
     8161 aatacgatcc attattaaac ggtgcgccaa ttaccgaacc tgtcgctgta gcagctgctg
     8221 ctaccacggc gacacaaagc tgggctgcgc cggttgaacc tgtgactcag acgccgcctg
     8281 ttgcctctgt tgatgttcca ccttcgcaac ctacagtagc ctggcagcct gtaccgggtc
     8341 cacaaacggg agagccggtt attgctcctg caccggaagg ttacccacag cagtcacaat
     8401 atgcgcagcc tgcagtgcaa tataatgagc cgctgcaaca accagtacag ccgcagcagc
     8461 cgtattatgc acctgcagct gaacaacctg cgcaacagcc gtattatgcc cctgcgccag
     8521 aacaaccggt ggcaggtaac gcctggcaag ccgaagagca gcaatccact tttgctccac
     8581 agtctacata ccagactgag caaacttatc agcagccagc cgctcaggag ccgttgtacc
     8641 aacagccgca acccgttgaa cagcagcctg ttgtggagcc tgaacccgtt gtagaagaga
     8701 caaaacccgc gcgtccgccg ctttactact ttgaagaagt tgaagagaag cgagcccgtg
     8761 aacgtgaaca acttgcggcc tggtatcaac cgattccaga accggttaaa gaaccagaac
     8821 cgatcaaatc ttcgctgaaa gcaccttctg ttgcagcagt acctccagta gaagccgctg
     8881 ccgctgtttc tccgctggca tctggcgtga aaaaagcgac actggcgacg ggggctgccg
     8941 caaccgttgc cgcgccagtc ttcagtctgg caaatagcgg tggaccgcgt ccgcaggtca
     9001 aagaggggat tggcccgcag ttgccacgac cgaaacgtat ccgcgtgcca actcgtcgtg
     9061 aattggcgtc ttacggtatt aagctgccct cacagcgtgc ggcggaagaa aaagcccgtg
     9121 aagcccagcg caatcagtac gattctggcg atcagtacaa cgatgatgaa atcgatgcga
     9181 tgcagcagga tgaactggca cgtcagttcg cccagacaca gcagcaacgc tatggcgaac
     9241 agtatcaaca tgatgtgccc gtaaatgcag aagatgcaga tgctgcggca gaggctgaac
     9301 tggctcgtca gtttgcgcaa actcaacaac aacgttattc cggcgaacaa ccggctgggg
     9361 cgaatccgtt ctcgctggat gattttgaat tttcgccaat gaaagcgttg ctggatgatg
     9421 gtccacacga accgctgttt acgccaattg ttgaacctgt acagcagccg caacaaccgg
     9481 ttgcaccgca gcagcagtat cagcagccac aacagccggt tgcgccacag ccgcagtatc
     9541 agcagccaca acagcaggtt gcgccgcagc cacaatatca gcagccgcaa caaccggttg
     9601 cgccacagca gcaatatcag cagccgcaac aaccagttgc gccgcagccg cagtatcaac
     9661 agccgcaaca accggttgca ccgcagccgc agtatcagca gccacaacag ccagttgcgc
     9721 cacagccgca ggatactctg cttcatccgc tgttgatgcg taatggcgac agccgtccgt
     9781 tgcataaacc gacgacgccg ctgccttctc tggatttgct gacaccgccg ccgagcgaag
     9841 tggagccggt agataccttt gcgcttgaac aaatggctcg cctggtggaa gcgcgtctgg
     9901 ctgatttccg tattaaagcc gatgtcgtca attactctcc ggggccggtt atcactcgct
     9961 ttgaacttaa cctggcaccg ggcgtaaaag cggcgcgcat ttctaacttg tcacgggacc
    10021 ttgctcgttc actttcgacg gtggcggtgc gtgtcgttga agttattcct ggcaaaccct
    10081 atgtaggtct ggagttaccg aataaaaaac gacaaaccgt ttatctgcgc gaagtattgg
    10141 ataacgccaa attccgcgat aatccatcgc cattaaccgt ggtgctgggt aaagatatcg
    10201 ccggtgagcc ggtggttgcc gatctggcga aaatgccgca cttgttagtt gcggggacta
    10261 ccggttccgg taaatctgtc ggtgtgaacg cgatgatcct gagcatgctt tataaagcac
    10321 agccagaaga tgtgcgtttc atcatgatcg acccgaaaat gctggagctt tcggtgtatg
    10381 aaggcattcc gcacctgtta acggaagtcg ttactgatat gaaagatgcc gccaacgcgc
    10441 tgcgctggtg tgttaacgag atggagcgtc gttataaact gatgtctgcg ctgggtgtgc
    10501 gtaatctggc gggttataac gaaaaaattg ctgaagccga tcgcatgatg cgtccgattc
    10561 cagacccgta ctggaagccg ggtgacagta tggatgccca gcatccggtg ctgaaaaaag
    10621 aaccatacat tgtggtgttg gtggacgaat ttgccgacct gatgatgaca gtaggtaaaa
    10681 aagtggaaga gctgatagca cgtctggcgc aaaaagcccg tgccgcgggt atccacctcg
    10741 tactggcaac tcagcgtcca tcggttgatg ttattactgg tctgattaaa gcgaatattc
    10801 cgacccgtat cgcctttacc gtatccagta agattgactc acgtaccatt cttgatcagg
    10861 ctggcgcgga atcactgctg ggtatggggg atatgctcta ctctgggccg aactccacgt
    10921 tgccggtacg tgtccatggt gcttttgttc gcgatcagga agttcatgcc gtggtgcagg
    10981 actggaaagc gcgtggtcgc ccacagtatg ttgatggcat cacctccgac agcgaaagcg
    11041 aaggtggtgc gggtggtttc gatggcgctg aagaactgga tccgttgttc gatcaggcgg
    11101 tgcagtttgt cactgaaaaa cgcaaagcgt caatttctgg cgtacaacgt cagttccgca
    11161 ttggttataa ccgtgcagcg cgtattatcg aacagatgga agcgcagggg attgtcagcg
    11221 aacaggggca caacggtaat cgtgaagtgc tgtccccacc gccgtttgac taattaatgc
    11281 attgccggat aaggcgcggt agcgtcgcat ccggcactct atcaactgaa aattcagtat
    11341 tttcttcttt cctcaagctg attattagcc tggaatagag agtagaggga actcccgatc
    11401 gggagtgacg taatttgagg aataatgatg aaaaaaattg ccatcacctg tgcattactc
    11461 tcaagcttag tagcaagcag cgtttgggct gatgccgcaa gcgatctgaa aagccgcctg
    11521 gataaagtca gcagcttcca cgccagcttc acacaaaaag tgactgacgg tagcggcgcg
    11581 gcggtgcagg aaggtcaggg cgatctgtgg gtgaaacgtc caaacttatt caactggcat
    11641 atgacacaac ctgatgaaag cattctggtt tctgacggta aaacactgtg gttctataac
    11701 ccgttcgttg agcaagctac ggcaacctgg ctgaaagatg ccaccggtaa tacgccgttt
    11761 atgctgattg cccgcaacca gtccagcgac tggcagcagt acaatatcaa acagaatggc
    11821 gatgactttg tcctgacgcc gaaagccagc aatggcaatc tgaagcagtt caccattaac
    11881 gtgggacgtg atggcacaat ccatcagttt agcgcggtgg agcaggacga tcagcgcagc
    11941 agttatcaac tgaaatccca gcaaaatggg gctgtggatg cagcgaaatt taccttcacc
    12001 ccgccgcaag gcgtcacggt agatgatcaa cgtaagtaga ggcacctgag tgagcaatct
    12061 gtcgctcgat ttttcggata atacttttca acctctggcc gcgcgtatgc ggccagaaaa
    12121 tttagcacag tatatcggtc agcaacattt gctggctgcg gggaagccgt tgccgcgcgc
    12181 tatcgaagcc gggcatttac attctatgat cctctggggg ccaccgggta ccggcaaaac
    12241 aaccctcgct gaagtgattg cccgctatgc gaacgctgat gtggaacgta tttctgccgt
    12301 cacctctggt gtgaaagaga ttcgcgaggc gatcgagcgc gcccggcaaa accgcaatgc
    12361 aggtcgccgc actattcttt ttgttgacga agttcaccgt ttcaacaaaa gccagcagga
    12421 tgcatttctg ccacatattg aagacggcac catcactttt attggcgcaa ccactgaaaa
    12481 cccgtcgttt gagcttaatt cggcactgct ttcccgtgcc cgtgtttatc tgttgaaatc
    12541 cctgagtaca gaggatattg agcaagtact aactcaggcg atggaagaca aaacccgtgg
    12601 ctatggtggt caggatattg ttctgccaga tgaaacacga cgtgccattg cagaactggt
    12661 gaatggcgac gcgcgccggg cgttaaatac gctggaaatg atggcggata tggccgaagt
    12721 cgatgatagc ggtaagcggg tcctgaagcc tgaattactg accgaaatcg ccggtgaacg
    12781 tagcgcccgc tttgataaca aaggcgatcg cttttacgat ctgatttccg cactgcataa
    12841 gtcggtacgt ggtagcgcac ccgatgcggc gctgtactgg tatgcgcgaa ttattaccgc
    12901 tggtggcgat ccgttatatg tcgcgcgtcg ctgtctggcg attgcgtctg aagacgtcgg
    12961 taatgccgat ccacgggcga tgcaggtggc aattgcggcc tgggattgct ttactcgcgt
    13021 tggcccggcg gaaggtgaac gcgccattgc tcaggcgatt gtttacctgg cctgcgcgcc
    13081 aaaaagcaac gctgtctaca ctgcgtttaa agccgcgctg gccgatgctc gcgaacgccc
    13141 ggattatgac gtgccggttc atttgcgtaa tgcgccgacg aaattaatga aggaaatggg
    13201 ctacgggcag gaatatcgtt acgctcatga tgaagcaaac gcttatgctg ccggtgaggt
    13261 ttacttcccg ccggaaatag cacaaacacg ctattatttc ccgacaaaca ggggccttga
    13321 aggcaagatt ggcgaaaagc tcgcctggct ggctgaacag gatcaaaata gccccataaa
    13381 acgctaccgt taatgttatc gttgcggtaa tgttgttact gtatccctgt ggtcgcaggc
    13441 tgtggccaca tctcccattt aattcgataa gcacaggata agcatgctcg atcccaatct
    13501 gctgcgtaat gagccagacg cagtcgctga aaaactggca cgccggggct ttaagctgga
    13561 tgtagataag ctgggcgctc ttgaagagcg tcgtaaagta ttgcaggtca aaacggaaaa
    13621 cctgcaagcg gagcgtaact cccgatcgaa atccattggc caggcgaaag cgcgcgggga
    13681 agatatcgag cctttacgtc tggaagtgaa caaactgggc gaagagctgg atgcagcaaa
    13741 agccgagctg gatgctttac aggctgaaat tcgcgatatc gcgctgacca tccctaacct
    13801 gcctgcagat gaagtgccgg taggtaaaga cgaaaatgac aacgttgaag tcagccgctg
    13861 gggtaccccg cgtgagtttg actttgaagt tcgtgaccac gtgacgctgg gtgaaatgca
    13921 ctctggcctc gactttgcag ctgcagttaa gctgactggt tcccgctttg tggtaatgaa
    13981 agggcagatt gctcgcatgc accgcgcact gtcgcagttt atgctggatc tgcataccga
    14041 acagcatggc tacagtgaga actatgttcc gtacctggtt aaccaggaca cgctgtacgg
    14101 tacgggtcag ctgccgaaat ttgctggcga tctgttccat actcgtccgc tggaagaaga
    14161 agcagacacc agtaactatg cgctgatccc aactgcagaa gttccgctga ctaacctggt
    14221 gcgcggtgaa atcatcgatg aagatgatct gccaattaag atgaccgccc acaccccatg
    14281 cttccgttct gaagccggtt catatggtcg tgacacccgt ggtctgatcc gtatgcacca
    14341 gttcgacaaa gttgaaatgg tgcagatcgt gcgcccggaa gactcaatgg cggcgctgga
    14401 agagatgact ggtcatgcag aaaaagtcct gcagttgctg ggcctgccgt accgtaaaat
    14461 catcctttgc actggcgaca tgggctttgg cgcttgcaaa acttacgacc tggaagtatg
    14521 gatcccggca cagaacacct accgtgagat ctcttcctgc tccaacgttt gggatttcca
    14581 ggcacgtcgt atgcaggcac gttgccgcag caagtcggac aagaaaaccc gtctggttca
    14641 taccctgaac ggttctggtc tggctgttgg tcgtacgctg gttgcagtaa tggaaaacta
    14701 tcagcaagct gatggtcgta ttgaagtacc agaagttctg cgtccgtata tgaacggact
    14761 ggaatatatt ggctaatacc caatttttct gaatctaaaa agcgcctacg ggcgcttttt
    14821 ttgtctccct ttgataccga acaataatta ctcctcactt acacgtaata ctactttcga
    14881 gtgaaaatct acctatctct ttgattttca aattattcga tgtatacaag cctatatagc
    14941 gatctgctat agaaataatt acacaatacg gtttgttact ggaatcaatc gtgagcaagc
    15001 ttgagtgagc cattatgaaa acgaaaatcc ctgatgcggt attggctgct gaggtgagtc
    15061 gccgtggttt ggtaaaaacg acagcgatcg gcggcctggc aatggccagc agcgcattaa
    15121 cattaccttt tagtcggatt gcgcacgctg tcgatagcgc cattccaaca aaatcaggcg
    15181 aaaaggttat ctggagcgcc tgtacagtta actgtggtag tcgctgcccg ctacgtatgc
    15241 acgtcgtgga cggtgaaatc aaatatgtcg aaacggacaa taccggcgat gacaattacg
    15301 acggcctgca ccaggttcgc gcctgcctgc gtgggcgttc catgcgtcgc cgtgtctaca
    15361 atccggaccg cctgaaatat ccgatgaaac gagtcggggc gcgcggtgaa ggcaaattcg
    15421 agcgcattag ctgggaagaa gcctacgaca tcatcgcgac caatatgcag cgcctgatca
    15481 aagagtacgg caacgaatcc atctatctga actatggcac cggtacgctg ggtggcacca
    15541 tgacccgctc ctggccgccg ggaaataccc tggtcgcgcg gctgatgaac tgctgcggcg
    15601 gctatctgaa ccattacggc gactactcct ccgcgcaaat tgcggaaggt ctgaactata
    15661 cctacggcgg ctgggcagat ggcaacagcc cgtcggatat cgaaaacagt aagctggtag
    15721 tgctgtttgg taataaccct ggcgaaacgc gaatgagtgg cggtggggtg acttactatc
    15781 ttgaacaggc acgccagaaa tctaatgccc gcatgatcat catcgatccg cgctataccg
    15841 acaccggtgc cgggcgcgaa gatgagtgga tccctattcg tccgggaaca gatgccgcac
    15901 tggttaacgg tctggcgtac gtcatgatca ctgaaaacct ggtggatcag gcattcctcg
    15961 ataaatattg cgttggctac gatgagaaaa ccctgccagc cagtgcgccg aaaaatggcc
    16021 actataaagc ttatattctg ggtgaagggc cagatggcgt ggctaaaaca ccggaatggg
    16081 cctcgcaaat caccggtgtt ccggcagaca aaatcatcaa actggctcgt gaaatcggca
    16141 gtaccaaacc ggcgtttatc agccagggat ggggcccgca gcgtcacgct aacggtgaaa
    16201 tcgcaacccg tgctatctcg atgctggcga ttctgaccgg taacgttggt attaatggag
    16261 ggaacagcgg cgcgcgtgaa ggttcataca gtttaccgtt tgtccgtatg ccgaccttgg
    16321 aaaacccgat ccagaccagc atttcgatgt ttatgtggac cgatgccatt gaacgtggcc
    16381 cggaaatgac ggcgctacgt gatggtgtgc gcgggaaaga taagctggat gtgccgatca
    16441 aaatgatctg gaactatgcc ggtaactgcc tgattaacca gcattctgaa atcaaccgta
    16501 cccatgaaat ccttcaggat gataagaagt gcgagctgat tgtggttatc gactgccaca
    16561 tgacctcatc ggcgaaatat gctgacatcc tgctgcctga ctgcaccgct tccgaacaga
    16621 tggactttgc actggatgca tcctgcggga atatgtctta cgtgattttc aacgatcagg
    16681 tgattaaacc gcgctttgaa tgtaagacca tctatgaaat gaccagcgaa ctggcaaaac
    16741 gtcttggcgt tgagcaacag tttactgaag gccgtaccca ggaagagtgg atgcggcatc
    16801 tgtatgccca gtcgcgggaa gcgattcctg aactgccaac gtttgaagag ttccgcaagc
    16861 aggggatctt taaaaagcgc gacccacaag ggcatcacgt tgcttataaa gccttccgtg
    16921 aagatccgca ggcaaatcca ctgaccacgc catcgggtaa aattgagatt tattcgcagg
    16981 cgctggctga cattgccgct acctgggaat tgccagaagg cgatgtgatc gatccactgc
    17041 cgatctacac gccgggcttt gaaagttatc aggatccgct gaacaaacag tatccgctgc
    17101 agcttacagg tttccactat aaatctcgcg ttcactcaac ttacggcaac gttgatgtgc
    17161 tgaaagcggc ttgccgtcag gaaatgtgga tcaacccgct tgatgcccaa aaacgcggta
    17221 tccacaacgg cgataaagtc aggatcttta acgatcgtgg tgaggttcat attgaggcga
    17281 aagtgacgcc acgaatgatg ccgggtgtgg tcgcactggg tgaaggtgcc tggtatgacc
    17341 cggatgcaaa acgtgtcgat aagggtggtt gtattaacgt actgaccact caacgtccgt
    17401 ctcctctcgc taaggggaat ccgtcacata caaaccttgt tcaggttgaa aaggtgtaag
    17461 gagtaaccga tgacaaccca gtatggattt tttattgatt ccagccgttg caccggttgc
    17521 aaaacctgcg agctggcttg taaagactac aaagatttga tgccagaagt cagcttccgc
    17581 cgcatttatg aatatgctgg tggcgactgg caggaagata acggtgtctg gcaccagaac
    17641 gtgtttgcct actatctgtc gatttcgtgc aaccactgcg aagatccggc ttgtactaaa
    17701 gtctgcccga gcggtgcgat gcataaacgt gaagatggtt ttgttgtggt cgatgaagat
    17761 gtgtgcattg gctgccgcta ctgccatatg gcttgcccgt atggcgcacc gcaatataac
    17821 gaaacgaaag gccatatgac caaatgcgat ggttgttatg accgtgttgc ggagggtaaa
    17881 aagccgatct gtgttgaatc ttgtccgctg cgggcgctgg atttcggccc tatcgacgag
    17941 ctgcgtaaaa aacatggcga tctggcggcc gttgcgccgt tgccgcgagc tcactttacc
    18001 aaaccgaata ttgtgatcaa acccaatgcc aatagccgcc cgaccgggga taccactggc
    18061 tatctggcaa acccgaagga ggtgtgagat gggaagtgga tggcatgaat ggccgctgat
    18121 gatcttcacg gtcttcgggc aatgtgtggc gggtggtttt atcgttctgg ctttggcgct
    18181 gcttaaaggc gacctgcgag cagaagccca gcagcgtgtt atcgcctgca tgtttggctt
    18241 atgggtgctg atgggcattg gctttatcgc ctctatgctc catcttggtt caccgatgcg
    18301 cgcctttaac tcgctcaacc gggtaggggc ttcagcactc agtaacgaaa tcgccagcgg
    18361 ttcgatcttt tttgctgtag gcggcatcgg ctggttgctg gcaatgctga aaaagctgtc
    18421 acctgcattg cgtacgctat ggctggtggt gacgatggtt cttggcgtcg tctttgtctg
    18481 gatgatggtg cgtgtgtata acagcattga taccgttccg acctggtaca gcatctggac
    18541 gccgatgggc ttcttcctga cgatgttcat gggcggcccg ttactgggtt acctgttgct
    18601 gagtctggct ggcgtcgatg gctgggcgat gcgtctgctg ccagccattt ctgtactggc
    18661 actggtggtg agtggcgtga tgtcagtgat gcagggcaca gagctggcaa ctattcatag
    18721 ttctgtgcag caggccgcag cgctggtgcc ggactatggt gcgctgatgt cctggcgaat
    18781 cgtgcttttg gccgttgccc tgtgcttgtg gattgcacca cagctaaaag gttatcagcc
    18841 tgcggttccg ctactatccg tctcattcat tctgcttctg gcaggggaat taatcggtcg
    18901 cggcgtattc tatggtttgc atatgaccgt ggggatggcc gtcgcaagct aatcagtaac
    18961 aaccggggtt tcggccccgg ttctctttta tttctgcttc gttaacgtgt cataactggt
    19021 catcaaatta cgataatcag gaatgtggtt agagaacaac gtcgccagtc cttcaatatc
    19081 attacgccag tcacggtgca gctcgcaagc cacgccgaac catgtcatca attgcgcacc
    19141 tgcttgcgac atgcgatccc atgccgaatg ccgggtaatt tcattaaagg tccctgacgc
    19201 gtcagtcaca acaaagacat caaacccttc ttcaatggct gaaagcgccg ggaatgccac
    19261 gcaaacttcg gttaccacac cggcaataat taactgtttt ttacctgtcg ctttgactgc
    19321 ttttacaaaa tcttcgttat cccaggcgtt aatattgcca gggcgagcaa tgtaaggtgc
    19381 atcggggaat tgtgctttca gctctggaac taatgggccg ttaggaccgg tttcaaaact
    19441 ggtggtaaga atagttggta agttgaaata cttagccagg tcacccagcg ccagcacgtt
    19501 atttttaaac ttatcgggtt cgatatcccg tacaagggaa agtaaaccag cctggtgatc
    19561 aacaagcaaa acggcagcat catttttatc aagacgaaca tacggtttgg tcatcctctt
    19621 ctcctttcga gagtcagtgg tacgtctgaa atcggtcaga cgcgatgcat tgctctgaaa
    19681 gcatagacgg gaaatatgag tttgctgtga ccatgaaatt tttcgactga acgaagcgta
    19741 tgaaatttgt gttagatcaa taaaaacaat cagatgagat taatcatctc ttcagcatta
    19801 gcgttatgaa tgagtaatat aaatcgataa taccgccgct gcgatatgcg cgtattttca
    19861 cccattgaca atgttttttg cggtggcatg atgcgcatga aatttgaact tcctcacggt
    19921 tttaattcat gtccacgtat acccggcctg tcatgctttt gctgtctggc ctgcttttgt
    19981 tgactctggc gatcgcggtg ttaaatacac tcgtgccgct ttggctcgcc caggaacaca
    20041 tgtccacatg gcaggtaggc gttgtcagct catcctattt taccggcaac cttgtcggta
    20101 cattgctgac agggtatgtc attaaacgca ttggctttaa ccgcagctat tatctggcct
    20161 ccttcatttt tgccgctggc tgtgccggcc ttggcctgat gattggtttc tggagctggt
    20221 tggcgtggcg ttttgtcgcg ggcgtcggct gtgccatgat ttgggtagtc gttgagagcg
    20281 cgctgatgtg cagtgggacg tcacgtaacc gtgggcgttt gcttgctgcg tatatgatgg
    20341 tttattacgt gggaacattt ttaggccagt tactggtcag caaagtctca accgagctga
    20401 tgagcgtatt gccgtgggtt acaggtttga cgttggcagg gatcttaccg ctgttgttta
    20461 cgcgtgtcct gaatcagcag gctgaaaacc atgattcgac gtcaattacg tcaatgctaa
    20521 aactccgtca ggcgcggctt ggcgtgaatg gctgcattat ctcaggaatc gtgctgggat
    20581 ctctatatgg cctgatgccg ctgtacctca atcacaaagg ggtgagcaat gccagcattg
    20641 gtttctggat ggcggtactg gtcagtgcgg gtatcctcgg acaatggccg attggacgtc
    20701 tggcggataa gtttggtcga ctgctggtgt tgcgtgttca ggtctttgtc gtcattctcg
    20761 gcagtatcgc gatgcttagc caggcggcga tggccccagc gttattcatc ctcggtgccg
    20821 ctggctttac gctatatccg gtggcgatgg cctgggcttg tgagaaagtt gaacatcatc
    20881 aactggtggc gatgaaccag gccttactgt tgagctatac cgtgggaagt ctgcttggcc
    20941 cgtcatttac cgctatgcta atgcagaatt tctccgataa tttattgttt atcatgatcg
    21001 ccagcgtatc gtttatctat ttgctgatgc tgcttcgcaa cgccggtcat acgccgaaac
    21061 ccgttgctca cgtgtaaatg aattcaagca aagtgtgaac ttactgtttc acactctgct
    21121 tttttgtttc ttctatctga cttgcttttt tccaaatttt attcgtttaa aaataaaatg
    21181 tgcagcaggt tataattttg catttcgcta tttccgcact tcttatttgc cgcgcataat
    21241 ccctcgtttt acagatgccc ctttaatttt ggcgaaggat ttgtctatgg ctgggaatgt
    21301 tcaggaaaaa cagctgcgat ggtacaacat tgcgctgatg tcttttatca ctgtctgggg
    21361 ttttggcaac gttgttaata actatgccaa ccaggggctg gtggttgttt tttcatgggt
    21421 gtttatcttt gcgctctatt tcacacctta tgcactaatt gttggtcagt taggctcgac
    21481 cttcaaagat gggaagggcg gggtcagtac ctggattaaa cacacgatgg gacccggatt
    21541 ggcttacctc gccgcgtgga cctactgggt ggtgcatatt ccctatctgg cacaaaaacc
    21601 ccaggcaata ctgattgcgc tcggttgggc gatgaaaggc gacggttcgc taatcaaaga
    21661 atattcagtc gtagcgttac aggggttaac gctggtgctg tttatcttct ttatgtgggt
    21721 tgcttcacgc ggtatgaaat cgctgaaaat cgtcggttct gtggcaggga ttgcgatgtt
    21781 tgttatgtcg ctcctgtatg tggcgatggc ggtaaccgcg cctgcgatta ctgaagtgca
    21841 tattgcgacc acaaacatta cctgggaaac gttcattcct catatcgact ttacctacat
    21901 taccactatt tcaatgctgg ttttcgcggt tggcggagct gagaagattt ctccttacgt
    21961 taatcaaacg cgcaacccag gaaaagaatt tccaaaaggg atgttatgcc tggcggtgat
    22021 ggttgcggtt tgtgccattc tgggctcact ggcgatgggg atgatgtttg actcgcgtaa
    22081 tatcccggat gacttaatga ctaacggtca gtattacgcc tttcagaagc tgggcgagta
    22141 ttacaacatg ggtaatactt taatggtgat ttacgccatt gcgaataccc tgggacaagt
    22201 agctgcgctg gtattctcga ttgatgcccc gcttaaagtg ctattaggcg atgctgacag
    22261 caaatatatt ccagccagtt tatgtcgtac caacgcttct ggtacgcccg ttaatggcta
    22321 ttttctgacc ctggtgctgg tggcgatcct gattatgctg ccgactctcg gcatcggtga
    22381 tatgaacaat ctctataaat ggctgttgaa tcttaactcg gtagtgatgc cgctgcgtta
    22441 tttatgggta tttgttgcat ttattgcagt cgttcgcctg gcgcagaaat atacaccaga
    22501 gtatgtcttt attcgtaata agcctctggc aatgaccgtc gggatttggt gttttgcctt
    22561 tactgccttt gcctgtttga cggggatctt cccgaaaatg gaagccttca ctgcagagtg
    22621 gaccttccag ttggcgctga atgttgcaac gccgtttgtg ctggtaggat taggactgat
    22681 attcccgctg ctggcgcgta aagcgaatag taaataatta ttgtggtcgg tgacgctgac
    22741 tctggcagat ctgacccgtt tttacataat cgagaaatgc gcgcaagccg caggacatat
    22801 actggcggtt tgggtaatac atttggaagc tgtgctgtaa gggcgttaga aatagagctc
    22861 tgaattattt atgcagcgcg gaataaaaaa gcggcaactc aataaagttg ccgctttacg
    22921 gggaaattag aacattacct tatgaccgta ctgctcaaga atacctttca cgcgttccat
    22981 ggtttctttc ttcggtggtt taacaccatc gagtttgtat tcttcaccca tcgccaccca
    23041 tttgtgtttg cccagttcgt ggtaggggag aagttcgatt ttctcaacgt tacccatatc
    23101 acgggtaaat tcaccgaggc gatgcgctga atcgtcatcg tcagaccagc ctgggacaac
    23161 aacgtagcgg atccacacct tcacattttt attcgccaga tatttggcga actccagcgt
    23221 gcggtggttg gaaactccaa ccagattttg gtggatctcg tcgttcatct gtttgagatc
    23281 gagcattacc aggtcggtta cttccagcag ttcatcaatc accggatcgt aacgacgaac
    23341 aaaaccgttg gtgtccagac aggtatgaat gccttctttt ttgcaggcgc ggaaccagtc
    23401 acgaacaaac tcagcttgca ggattgcttc accgccggat gcggtaacgc cgccgccgga
    23461 agcgttcata aagtggcgat aggtcaccac ttccttcatc aaatcttcaa cggtaacttc
    23521 tttaccgcca tgcgtgtccc aggtgtcgcg gttatgacaa tacaggcagc gcatcaggca
    23581 gccctggaaa aaggtgataa agcgaatacc tgggccgtct acggttccac aggattcaaa
    23641 ggagtgaatg cgaccaataa ctgacattgc ggtgtttctc cagatgtggc ccatctgagg
    23701 ccgtgttggt gcgcagctcg aaggctacgt cgagtctgtt ttggcagtca ccttaaagta
    23761 tagatagctg acaaaaaagg ctcacgtgct aaaaaaggcc ccactttcgt ggagccttta
    23821 ttgtacgctt tttactgtac gatttcagtc aaatctaatt acatagattg agtgaaggta
    23881 cgagtaataa cgtcctgctg ctgttcttta gtcagcgagt tgaaacgtac tgcgtagcca
    23941 gatacacgga tggtcagctg cggatatttt tccggatttt ccatcgcgtc gagcagcatt
    24001 tcacggttca tcacgttaac gttcaggtgc tgaccacctt cgatggatgc ttcgtggtgg
    24061 aagtaaccat ccatcagacc agccaggttg gtcttacgaa cttcgtcgtc tttacccagt
    24121 gcgttcggaa cgatagagaa ggtgtaggag ataccatctt tagcgtaagc aaacggcagt
    24181 ttagcaacgg aagtcagaga cgctacagca cctttctggt cacgaccgtg catcgggtta
    24241 gcacccggtc cgaacggcgc gccagcacga cgaccgtctg gggtgttacc cgttttctta
    24301 ccatacacaa cgttagaagt gatggtcaga acagactgag tcgggatagc gtcacggtag
    24361 gtgtgcagtt tctgaatttt cttcatgaaa cgttctacca ggtcaacagc caggtcatct
    24421 acacgcggat cgttgttacc aaactgcggg tattcgcctt cgatttcgaa gtcgatagcc
    24481 agaccgtctt cgtcacgaat cggtttaact ttcgcatatt tgattgcaga cagggagtca
    24541 gcagcaacgg acagaccagc gataccacac gccatggtgc ggataacgtc acggtcgtgc
    24601 agcgccatca gagaggcttc gtagctgtac ttgtcgtgca tgtagtgaat gatgttcagt
    24661 gcagtgatgt actgtttagc cagccagtcc atgaagtgat ccatgcgctc catcacttca
    24721 tcgtagttca ggagatcgcc tttgatcggt tcagacttcg gaccaacctg cattttcagt
    24781 ttttcgtcaa cgccgccgtt gattgcgtac agcatggttt tcgccaggtt tgcacgcgca
    24841 ccgaagaact gcatttgttt accaacgatc atcgggctta cgcagcaagc gatagcgtag
    24901 tcatcgttgt tgaagtccgg acgcatcagg tcatcgttct catactgcag agaagaggtg
    24961 tcgatggaca ctttagcggc gaatttcttg aagttcagcg gcagtttttc agaccacaga
    25021 atggtcatgt tcggttccgg agacggaccc atggtgtaca gggtgttcag gaaacggaag
    25081 ctgtttttgg taaccagggt acgaccgtcg aggcccatac caccgataga ttcggttgcc
    25141 cagatcgggt cgccagagaa cagttcatcg tactccggag tacgcaggaa gcgaaccata
    25201 cgcagtttca tgaccaggtg gtcaaccatt tcctgcgctt cttgttcggt gatcttgcca
    25261 gctttcaggt cacgttcgat gtacacatcc aggaaggtgg aggtacgacc gaaggacatt
    25321 gcagcaccgt tctgagactt aacagcagcc aggtagccga agtaagtcca ctggatagct
    25381 tcctgagcgt tggtagccgg accagagatg tcgtagccgt atttcgcagc catttctttc
    25441 atctgaccca gagcgcggtg ctgttcagcg atttcttcgc gcagacggat agtctgttcc
    25501 aggtttacgc cgttttccag atcagcctgc agagaggtga actgagcgta tttgtctttc
    25561 atcaggtagt cgataccgta cagcgcaacg cgacggtagt caccgatgat acggccacgg
    25621 ccataagcat ctggcagacc ggtcagaaca ccggatttac ggcaacgcag gatgtccgga
    25681 gtgtaaacgt cgaacacgcc ctggttgtga gttttacggt attcagtgaa gatttttttg
    25741 atcatcgggt ccagttcgcg gttgtacgct ttgcaggaac cttcgatcat tttgatacca
    25801 ccgaacggga taagagcacg tttcagcgga gcttcagtct gcagaccaac aactttttcc
    25861 aacgctttgt tgatgtagcc agcgtcgtga gaggtgatgg tggaagcaac agcggtgtcg
    25921 aagtcaactg gcgcgtgagt gcggttttcc agtttaacgc cttccattac tttgtcccac
    25981 agggtggtgg tcgcttcagt agcgccagcc aggaaggact cgtcaccctc gtacggagtg
    26041 tagtttttct gaatgaagtc acggacgttt acttcattct gccagtcacc tttggtaaaa
    26101 ccttcccagg ctgtggctaa cttttcatta agctcggaca tgtaacacct accttcttaa
    26161 gtggattttt tatttactgc gtacttcgac aaccattaat ggtgatcgtt ttcacgcagg
    26221 taaatgaccc agtatgtcaa cccaaccaac aaaccaccgc cgataatatt accgatcgta
    26281 accggaatca ggttatcagt gatgaagttc atcacggtca ggtgagaaaa attttccggt
    26341 gcagaaccga cagcggtcca gaattccgga gatgcgaagt cgcggattac aatacccatc
    26401 gggatcataa acatgtttgc gatactgtgc tcaaaaccgc tggcaacaaa catcgcgacc
    26461 ggcagcacca tgataaacgc tttgtccatc aggctgcggc cagaataact catccatact
    26521 gccagacata ccatcaggtt tgccaggata ccaaggcaga cggcctcaat aaaagtatgg
    26581 tgcactttgt ggtcggcggt ttgtaggacg tttagtcccc attgaccatt tgcggtcata
    26641 tactcgccgg aaagccacat taaaagtaca aacagcagtg cgccgaccag gttgccaaaa
    26701 tagacattta gccagttttt agccaactga ccccaggtga tgcgcccact cgccttagca
    26761 acaacaatca acactgtgga agtaaagaga tcggctccgc agacaacaca aagaatcagt
    26821 cccagagaga agcaaatgcc gccaaccagt tttgccatgc cgaagggcat tgtgcctgtg
    26881 ccagtggttg ctgtgatata gaagacgaat gcgattgaga tgaaaacacc ggcggtaatc
    26941 gccagataga aagtcttaag cggatgtttc gttgctttat agacacccgc ctcttcggcc
    27001 actttggcca ttgcagcagg aagtaaaaga tcaaaagggt tgtcagcttt cacactaact
    27061 ctctctttat taagtcggcg acgagatact aacaaagcat tatagatgag aaattgatat
    27121 agatcatatc tcgcctggct tataggccc
//
LOCUS       DAZKIP010000054        27021 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZKIP010000054 DAZKIP010000000
VERSION     DAZKIP010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27021
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.054"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(152..1816)
                     /gene="glnS"
                     /locus_tag="ACHOUK_002518"
     CDS             complement(152..1816)
                     /gene="glnS"
                     /locus_tag="ACHOUK_002518"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFP9897947.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRSD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(2019..3965)
                     /gene="nagE"
                     /locus_tag="ACHOUK_002519"
     CDS             complement(2019..3965)
                     /gene="nagE"
                     /locus_tag="ACHOUK_002519"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFP9897948.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4298..5098
                     /gene="nagB"
                     /locus_tag="ACHOUK_002520"
     CDS             4298..5098
                     /gene="nagB"
                     /locus_tag="ACHOUK_002520"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFP9897949.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5158..6306
                     /gene="nagA"
                     /locus_tag="ACHOUK_002521"
     CDS             5158..6306
                     /gene="nagA"
                     /locus_tag="ACHOUK_002521"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFP9897950.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6315..7535
                     /gene="nagC"
                     /locus_tag="ACHOUK_002522"
                     /gene_synonym="nagR"
     CDS             6315..7535
                     /gene="nagC"
                     /locus_tag="ACHOUK_002522"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFP9897951.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7583..8335
                     /gene="nagD"
                     /locus_tag="ACHOUK_002523"
     CDS             7583..8335
                     /gene="nagD"
                     /locus_tag="ACHOUK_002523"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFP9897952.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8592..10256
                     /gene="asnB"
                     /locus_tag="ACHOUK_002524"
     CDS             8592..10256
                     /gene="asnB"
                     /locus_tag="ACHOUK_002524"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFP9897953.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDKA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10636..10712
                     /locus_tag="ACHOUK_002525"
     tRNA            10636..10712
                     /locus_tag="ACHOUK_002525"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10670..10672,aa:Met,seq:cat)
     gene            10723..10807
                     /locus_tag="ACHOUK_002526"
     tRNA            10723..10807
                     /locus_tag="ACHOUK_002526"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10757..10759,aa:Leu,seq:tag)
     gene            10831..10905
                     /locus_tag="ACHOUK_002527"
     tRNA            10831..10905
                     /locus_tag="ACHOUK_002527"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10863..10865,aa:Gln,seq:ttg)
     gene            10940..11014
                     /locus_tag="ACHOUK_002528"
     tRNA            10940..11014
                     /locus_tag="ACHOUK_002528"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10972..10974,aa:Gln,seq:ttg)
     gene            11030..11106
                     /locus_tag="ACHOUK_002529"
     tRNA            11030..11106
                     /locus_tag="ACHOUK_002529"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11064..11066,aa:Met,seq:cat)
     gene            11155..11229
                     /locus_tag="ACHOUK_002530"
     tRNA            11155..11229
                     /locus_tag="ACHOUK_002530"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11187..11189,aa:Gln,seq:ctg)
     gene            11267..11341
                     /locus_tag="ACHOUK_002531"
     tRNA            11267..11341
                     /locus_tag="ACHOUK_002531"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11299..11301,aa:Gln,seq:ctg)
     gene            complement(11495..12670)
                     /gene="ubiF"
                     /locus_tag="ACHOUK_002532"
     CDS             complement(11495..12670)
                     /gene="ubiF"
                     /locus_tag="ACHOUK_002532"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFP9897954.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFTVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQVEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            12816..14240
                     /gene="miaB"
                     /locus_tag="ACHOUK_002533"
     CDS             12816..14240
                     /gene="miaB"
                     /locus_tag="ACHOUK_002533"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFP9897955.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14393..15433
                     /locus_tag="ACHOUK_002534"
     CDS             14393..15433
                     /locus_tag="ACHOUK_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFP9897956.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            15430..15897
                     /gene="ybeY"
                     /locus_tag="ACHOUK_002535"
     CDS             15430..15897
                     /gene="ybeY"
                     /locus_tag="ACHOUK_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFP9897957.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQMVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            15987..16865
                     /gene="corC"
                     /locus_tag="ACHOUK_002536"
     CDS             15987..16865
                     /gene="corC"
                     /locus_tag="ACHOUK_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFP9897958.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            16890..18428
                     /gene="lnt"
                     /locus_tag="ACHOUK_002537"
     CDS             16890..18428
                     /gene="lnt"
                     /locus_tag="ACHOUK_002537"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFP9897959.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIRWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            18825..19733
                     /gene="gltI"
                     /locus_tag="ACHOUK_002538"
     CDS             18825..19733
                     /gene="gltI"
                     /locus_tag="ACHOUK_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFP9897960.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            19903..20643
                     /gene="gltJ"
                     /locus_tag="ACHOUK_002539"
     CDS             19903..20643
                     /gene="gltJ"
                     /locus_tag="ACHOUK_002539"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFP9897961.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQASKLLDYSAHAWESFTAITLA
                     YVFINAFIMLVMTLVERKVRLPGNMGGK"
     gene            20643..21317
                     /gene="gltK"
                     /locus_tag="ACHOUK_002540"
     CDS             20643..21317
                     /gene="gltK"
                     /locus_tag="ACHOUK_002540"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFP9897962.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            21317..22042
                     /gene="gltL"
                     /locus_tag="ACHOUK_002541"
     CDS             21317..22042
                     /gene="gltL"
                     /locus_tag="ACHOUK_002541"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFP9897963.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            22160..23095
                     /gene="rihA"
                     /locus_tag="ACHOUK_002542"
     CDS             22160..23095
                     /gene="rihA"
                     /locus_tag="ACHOUK_002542"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFP9897964.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            23179..24849
                     /gene="hscC"
                     /locus_tag="ACHOUK_002543"
     CDS             23179..24849
                     /gene="hscC"
                     /locus_tag="ACHOUK_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFP9897965.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDEVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPIHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKRTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRQASVYLAIEIP"
     gene            complement(24909..26360)
                     /gene="djlC"
                     /locus_tag="ACHOUK_002544"
                     /pseudo
     CDS             complement(24909..26360)
                     /gene="djlC"
                     /locus_tag="ACHOUK_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
     gene            complement(26357..27019)
                     /locus_tag="ACHOUK_002545"
     CDS             complement(26357..27019)
                     /locus_tag="ACHOUK_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFP9897966.1"
                     /translation="MEILNQGCKPAHDSPKMYTGIKEFDLSSSWGINNRDDLIQTIYQ
                     MTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFVAQTAMCCGEGGIKAW
                     DYVRMGFLSRVGVLNKWLTEDESLWLQSRVYERAHHYYHSWMHYFSAYSLGRLYWQSS
                     QCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQPLTVQPECPVTLKDVS
                     DL"
BASE COUNT         6625 a         7022 c         6870 g         6504 t
ORIGIN      
        1 cggcctacaa aagcacgcaa actcaatata ttgcagagat catgtaggcc tgataagcgt
       61 agcgcatcag gcaatttagc gtttgaaact gtttgtctgt tccttgccgg atgcgacgta
      121 aacgccccat ccggcatagc gaaacttaaa attactcgcc tactttcgcc caggtatcac
      181 gcagcccaac ggtgcggtta aataccggtt tttccgccgt agaatggcgg ctatcgaggc
      241 agaagtaacc ttcacgctca aactggaatg ctttacccgc aaccgcatct ttcagcgacg
      301 gttcagcaaa gccctgtttg atcaccagcg attccgggtt aatcaccgac aggaaatcat
      361 ccgcagcacc tgggttcggc acgctgaaca gacggtcata caaacggatt tcaaccggca
      421 gcgcatgtgc cgcgctcacc cagtgaataa cacctttgac tttacgacca tctgccggat
      481 ctttgcttaa ggtatcggcg tcataagtac agaagatggt ggtgatatta ccttcggcat
      541 ctttctcgac gcgttctgcc ttaatcacat aagcattacg cagacgcact tctttaccca
      601 gcaccagacg tttgtactgc ttgttagctt cttcgcggaa atcggagcga tcaatccaaa
      661 tctcaccgct aaacggcacc tgacggctcc ccatttccgg tttgttcgga tggttcggca
      721 tggtaaccat ttcgccttcg ccctgatagt tttcgataac cagtttcacc ggatcgataa
      781 ccgccattgc gcgcggcgca ttttcgttga gatcttcacg gatgcaggat tccagcgacg
      841 ccatctcaat ggtgttgtcc tgcttggtca cgccgatgcg tttgcagaac tcacgaatag
      901 aagccgcagt gtaaccacga cgacgcagac cggaaatggt cggcatacgc gggtcatccc
      961 agccttcaac gtgcttgtcg gtcaccagca agttcagctt acgcttggac atcacagtgt
     1021 attccagatt caggcgcgag aattcgtact ggcgcgggtg aacaggaatg gtgatgttgt
     1081 ccagtaccca gtcgtacaga cgacggttat cctggaactc aagcgtacac agagagtgcg
     1141 taataccttc cagggcatcg ctgatgcagt gggtgaagtc gtacatcggg tagatgcacc
     1201 acttgttgcc agtctggtgg tgttcagcaa atttaatgcg gtacagcacc ggatcgcgca
     1261 tcacgataaa cggtgaagcc atgtcgattt tcgcacgcag gcaggcttta ccttcttcaa
     1321 aaccgccggt acgcattttc tcgaacagcg ccaggttctc ttcaacgcta cggtcgcggt
     1381 acgggctgtt tttaccaggc tgcgtcaggg tgccgcggta ttcgcggatc tgttccggcg
     1441 tcagttcatc aacgtacgcc aggcctttat tgatcagttc gatcgcatag gcgtggagct
     1501 gatcaaaata atcggaggag taacggacgt taccagacca gtgaaaacct aaccactcaa
     1561 cgtcgttttt gatcgaatcg acatactcga tatcttcttt taccgggtta gtgtcgtcga
     1621 aacgcaggtt gcactggcct ttatagtcct gggcgatccc gaagttcagg cagatagatt
     1681 tcgcatggcc aatatgcaga tagccattcg gctccggcgg gaaacgggtg tgtactgtgg
     1741 tgtgcttacc actggccaga tcttcatcga tgatctgacg gataaagtta gtcgggcggg
     1801 cttctgcctc actcatcgtg gattcctcaa agcgtaaaca acgtataacg gcgtatgatc
     1861 ttataagcgg gacaggctga caactgttag ttagtgaaaa tcgaaaatct cactgagatt
     1921 atggggatga ttgatgaaaa aaaaagcggc agagaagatc tccaccgctt gtcattgttg
     1981 gatgcgacgc tcaagcgtcg catcaggcat aaagcagatt actttttgat ttcatacagc
     2041 ggtgtttgac ccgccacaac atggccctga gctttaatga tcaagccact gaaatcgtcg
     2101 atattgctgc aaaccaccgg gctaatcatc gaacgggcgt tagcgttcag gtaatccaga
     2161 tccatttcca gaatcggttg ccctgcgctt acctgcgcgc cctcttccac cagacgttta
     2221 aagcctttac cttccagcgc tacggtatcg atacccatgt gaacgacaat ctccgcgcct
     2281 ttttcggttt ccaggcagaa cgcgtggttg gtgttgaaga ttttcacgat tgttcctgcg
     2341 gctggtgata cgacgatttt atctgtcggt ttcaccgcca caccgtcacc caccgctttg
     2401 ctggcgaatg cttcgtcagg aacctgatcc agtgccacga catcaccggt aatcggcgat
     2461 accagctccg cgatagatac cgcgtttggt acagcctgcg gttttgctac aggagcggca
     2521 gttgccggag ttgcttcagc agaggcagct gctaccgggc cacgggcaac gactttcttc
     2581 atcgcatcgc cgatggattc tgctttcgcg ccaacaatca cctgaatagt ctgtttgttc
     2641 agttttacta ccccagaagc ccccagacgt ttacacatcg tatcgttaac gcgggcagag
     2701 tcagccacgg taaggcgcag acgggtgata caggcgtcaa ttgctttcag gttgtcagtg
     2761 ccgccaaccg cagcaatata gttggtcgcc agttgattga ggccttcttc agtgttgctg
     2821 ttggcttctt cagtaacgat ctcgtcttct ttatcttcac gacccggcgt tttcaggttg
     2881 aacatgcgga taaccaaact gaacaccacg aagtagatag cgaagaagac aacacccata
     2941 accagcagca tccagacgtt ctggctggcg gccggcaggt tatacatcaa cgcgtagtcg
     3001 atagcccccg cagagaaaga gaagccggca tggataccca gcagcgttgc cacaaacagg
     3061 ctgataccgg tcagcagtgc gtgcaggagg tacagcagcg gagcaaggaa catgaacagg
     3121 aattccagcg gctcagtcac accggtcagg aacgcagtaa cagcaacaga aagcagcatc
     3181 ccgccaacca tcggacgacg ctctttcggt gctgcgaagt acatcgccag cgccgcaccc
     3241 ggcaggccga acatcatgat cgggaagaag ccggacatga acatccccgc ggtgccgtca
     3301 ccagcgtaga aacggttgat gtcgccgtgg aaaaccgtac ccgccgcgtt ggtgaattca
     3361 ccaatctgga accaggcgat ggtgttcagc acctgatgca gaccggttgg gatcaacaga
     3421 cggttgatga aaccaaagat accggaaccc agcgcgcccg cagaaacgat ccactcgccg
     3481 cctgcatgga tagcgtgctg taccggcggc cagacgtaac caaaaatggc cgccagcacc
     3541 agacagaaga agccggtggc gatcggcaca aagcgtttgc cgccgaagaa gctcaggaag
     3601 tccggcagtt taatatcgga ccaacggtta taggctgcgc caccaaccag accggtaatg
     3661 atacccgcca gtacacccat gttaatttct gggttgatgg tcaccatcgc tttggttaac
     3721 acaaagtaac ctaccgcccc cgccagcgcc gccgcacctg cgctgtcttt cgaccagctg
     3781 gatgccacac cgatggcgaa gattaatgcg aggttatcaa aaatcgcacc gcccgcctgg
     3841 gcaataaacg caacgttaag taaatctggc tgaccgaatc gcagcaacag tgccgccacc
     3901 ggcagcaccg cgatagggag ctgtaacgcc ctaccgagtc gctggaaaaa acctaaaata
     3961 ttcatcttat tccccctacg agaaccctat ttggctcgtt taaagccgta tttttattgt
     4021 gctgcaaatt gtactgccga tgttctgtaa tcagattgtt agatcatctg ccacagagtg
     4081 tgtgaaaatt taattcgtat cgcaaattaa acgagtgtct tttgtgagtt ttgtcaccaa
     4141 atatcgttat catcactccc ttttactggc taaaccagaa aacttatttt atcattcaaa
     4201 aaatcaggtc ggattgacgc ctgtctgcgc aaatccaggt tacgcttaaa gatgcctaat
     4261 ccgccaacgg cttacatttt acttattgag gtgaataatg agactgatcc ccctgactac
     4321 cgctgaacag gtcggcaaat gggctgctcg ccatatcgtc aatcgtatca atgcgttcaa
     4381 accgactgct gatcgtccgt ttgttctggg cctgccaact ggcggcacgc caatgaccac
     4441 ctataaagcg ttagtcgaaa tgcataaagc aggccaggtc agctttaagc acgttgtcac
     4501 cttcaacatg gacgaatatg tcggtctgcc gaaagagcat ccggaaagct actacagctt
     4561 tatgcaccgt aatttcttcg atcacgttga tattccagca gaaaacatca accttctcaa
     4621 cggcaacgcc ccggatatcg acgccgagtg ccgccagtat gaagaaaaaa tccgttctta
     4681 cggaaaaatt catctgttta tgggcggtgt aggtaacgac ggtcatattg catttaacga
     4741 accggcgtct tctctggctt ctcgtactcg tatcaaaacc ctgactcatg acactcgcgt
     4801 cgcgaactct cgtttctttg ataacgatgt caatcaggtg ccaaaatatg ccctgactgt
     4861 cggtgttggt acactgctgg atgccgaaga agtgatgatt ctggtgctgg gtagccagaa
     4921 agcactggcg ctgcaggccg cagttgaagg ttgcgtgaac catatgtgga ccatcagctg
     4981 tctgcaactg catccgaaag ccatcatggt gtgcgatgag ccttccacca tggagctgaa
     5041 agttaagact ttaagatatt tcaatgaatt agaagcagaa aatatcaaag gtctgtaatt
     5101 gttatccctg ccctggctcc ttgctcaggg caatattttt taaaatcggg ggtcagaatg
     5161 tatgcattaa cccagggccg gatctttacc ggccacgaat ttcttgatga ccacgcggtt
     5221 gttatcgctg atggcctgat taaaagcgtc tgtccggtag cggaactgcc gccagagatc
     5281 gaacaacgtt cactgaacgg ggccattctc tcccccggtt ttatcgatgt gcagttaaac
     5341 ggctgcggcg gcgtacagtt taacgacacc gctgaagcgg tcagcgtgga aacgctggaa
     5401 atcatgcaga aagccaatga gaaatcaggc tgtactaact atctgccgac gcttatcacc
     5461 accagcgatg agctgatgaa acagggcgtg cgcgttatgc gcgagtacct ggcaaaacat
     5521 ccgaatcagg cgttaggtct gcatctggaa ggcccgtggc tgaatctggt gaaaaaaggt
     5581 acccataatc cgaattttgt gcgtaagcct gatgccgcgc tggtcgattt cctgtgtgaa
     5641 aacgccgacg tcattaccaa agtgaccctg gcaccggaaa tggttcctgc ggaagtcatc
     5701 agcaaactgg caaatgccgg gattgtggtt tctgccggtc actccaacgc gacgttgaaa
     5761 gaagcgaaag ccggtttccg cgcggggatt acctttgcca cccatctgta caacgcgatg
     5821 ccgtatatta ccggtcgtga accgggcctg gcgggcgcga tcctcgatga agctgacatt
     5881 tattgcggta ttatcgctga tggcctgcat gttgattacg ccaacattcg taatgctaaa
     5941 cgccttaaag gcgacaaact gtgtctggtt accgacgcca ccgcgccagc aggtgccaac
     6001 attgaacagt tcatttttgc gggtaaaaca atatactacc gtaacggact ttgtgtggat
     6061 gagaacggta cgttaagcgg ttcatcctta accatgattg aaggcgtgcg taatctggtc
     6121 gaacattgcg gtatcgcact ggatgaagtg ctgcgtatgg cgacgctcta tccggcgcgt
     6181 gcgattggcg ttgagaaacg tctcggcaca ctcgccgcag gtaaagtagc caacctgacc
     6241 gcattcacac ctgattttaa aatcaccaag accatcgtta acggtaacga ggtcgtaact
     6301 caataagaga aagtatgaca ccaggcggac aagctcagat aggtaatgtt gatctcgtaa
     6361 aacagcttaa cagcgcggcg gtttatcgcc tgattgacca gtacgggcca atctcgcgga
     6421 ttcagattgc cgagcaaagc cagcttgccc ccgccagcgt aaccaaaatt acgcgtcagc
     6481 ttatcgaacg cgggctgatc aaagaagttg atcagcaggc ctccaccggg ggccgccgcg
     6541 ctatctctat cgtcaccgaa acccgcaatt tccacgcaat cggcgtacgg cttggtcgtc
     6601 acgacgccac catcaccctg tttgatctca gcagcaaagt gctggcagaa gaacattacc
     6661 cgctgccgga acgtacccag cagacgctgg aacatgccct gctgaatgcc attgctcagt
     6721 ttattgatag ctaccagcgc aaactgcgcg agctgatcgc catttccgtt atcctgccag
     6781 gacttgttga cccggacagc ggcaaaattc attacatgcc gcatattcag gtagaaaact
     6841 gggggctggt agaagcactg gaagagcgtt ttaaagtgac ctgtttcgtt ggtcacgata
     6901 tccgtagtct ggcgctggcg gagcactact tcggtgcaag tcaggattgc gaagactcca
     6961 ttctggtgcg tgtccatcgc ggaaccgggg ccgggattat ctctaacggg cgcattttta
     7021 ttggccgcaa cggcaacgtc ggtgaaattg gccatattca ggtcgaaccg ctgggtgaac
     7081 gctgccactg cggcaacttt ggctgcctgg aaactatcgc tgccaacgcc gctattgaac
     7141 aacgggtgtt gaatctgtta aagcagggct accagagccg cgtgccgctg gacgactgca
     7201 ccatcaaaac tatctgcaaa gccgcgaaca aaggcgatag cctggcctcg gaagtgattg
     7261 agtatgtcgg tcgtcatctg ggcaaaacca tcgccattgc tatcaactta tttaatccgc
     7321 aaaaaattgt tattgccggt gaaatcaccg aagccgataa agtgctgctc cctgctattg
     7381 aaagctgcat taatactcag gcgctgaagg cgtttcgtac taatctgccg gtggtacgtt
     7441 ctgagctaga ccaccgctcg gcaatcggtg cttttgcgct ggtaaaacgc gccatgctca
     7501 acggtatttt gctccagcat ttgctggaaa attaatgtgc ttttatagtg gcgcttattg
     7561 ttgtcaatat tctgggtagt ccatgaccat taaaaatgta atttgcgata tcgacggcgt
     7621 gctgatgcac gataacgtcg ccgtaccggg tgcagcggaa tttttgcacg ggattatgga
     7681 taaaggcctg ccgctggtgt tgctgaccaa ctatccttcg cagaccgggc aagatctggc
     7741 gaaccgcttt gccactgccg gtgtcgatgt acctgacagc gtgttttata cctctgcgat
     7801 ggcgaccgcc gatttcctgc gtcgccagga aggcaagaaa gcgtatgttg tgggcgaagg
     7861 cgcactgatt catgaactgt acaaagccgg tttcactatt accgatgtga accctgattt
     7921 tgtgattgtt ggcgaaacgc gttcctacaa ctgggacatg atgcataaag cggcctattt
     7981 cgtcgctaac ggtgcacgct ttatcgccac caacccggac acccacgggc gcggttttta
     8041 tccggcctgt ggcgcgttgt gtgcagggat tgagaaaatc tccgggcgca aaccgttcta
     8101 tgttggtaag ccgagtccgt ggattatccg cgcggcatta aacaaaatgc aggcacattc
     8161 ggaagaaacg gtgattgtcg gcgataacct gcgtaccgat attctggccg gcttccaggc
     8221 aggtctggag acgattctgg tgctttctgg tgtttcgtcg ctcgacgata tcgacagtat
     8281 gcctttccgt cccagctgga tttacccgtc agtcgctgaa atcgacgtta tctgaatagc
     8341 aaccacgtct caggacgtgg tttcttattt ttacccatca ttaaaatcat cccattcaat
     8401 aaaacccgcc aaaaattatc gatccatcaa taaatacatc accgcaccct cccctttttc
     8461 acaaatcagc aatcaccatt acgtttttta ttttttcacc gccaaatcgc ttgcgcacag
     8521 tgctgctttg cggcattttt ttaaacaagc aaacacaaca agcaacaaat accaggttaa
     8581 cggagaaggt tatgtgttca atttttggcg tattcgatat caaaacagac gcagttgagc
     8641 tgcgtaagaa agcactcgag ctgtcacgcc tgatgcgtca tcgtggcccg gactggtccg
     8701 gtatttatgc cagcgataaa gccattctcg ctcacgaacg tctgtcaatt gttgacgtta
     8761 acgcgggggc gcaacctctc tacaaccaac aaaaaaccca cgtactggcg gtaaacggtg
     8821 aaatctacaa tcaccaggca ttgcgcgctg aatatggcga tcgttatcag ttccagacag
     8881 ggtctgactg tgaagtgatc ctcgcgctgt atcaggaaaa agggccggaa tttctcgacg
     8941 acttgcaggg catgtttgcc tttgccctgt acgacagcga aaaagatgcc tacctgattg
     9001 gtcgcgacca tctggggatc atcccactgt atatgggcta tgacgaacac ggtcagctgt
     9061 atgtggcctc agaaatgaaa gccctggtgc cagtttgccg cacgattaaa gagttcccgg
     9121 cggggagcta tttgtggagt caggacggcg aaattcgttc ttattatcat cgcgactggt
     9181 tcgactttga tgcagtgaaa gataacgtga ccgacaaaaa cgagctgcgt caggctctgg
     9241 aagattccgt taaaagccat ctgatgtctg atgtgcctta cggtgtgctg ctttctggtg
     9301 gtctggattc ctcaattatt tccgctatca ccaagaaata cgcagcccgt cgcgtggaag
     9361 atcaggaacg ctctgaagcc tggtggccgc agttacactc ctttgctgta ggtctgccgg
     9421 gttcaccgga tctgaaagca gcccaggaag tggcaaacca tctgggcact gtgcatcatg
     9481 aaattcactt cactgtacag gaaggtctgg acgccatccg cgacgtgatt taccacatcg
     9541 aaacctatga tgtgaccact attcgcgctt caacaccgat gtatttaatg tcgcgtaaga
     9601 tcaaagcgat gggcattaaa atggtgctgt ccggtgaagg ttctgatgaa gtgttcggcg
     9661 gttatcttta cttccacaaa gcaccgaatg ccaaagaact gcatgaagag acggtgcgta
     9721 aactgctggc cctgcatatg tatgactgcg cgcgtgccaa caaagcgatg tcagcctggg
     9781 gcgtggaagc acgcgttccg ttcctcgaca agaaattcct cgacgtggcg atgcgcatta
     9841 acccgcagga taaaatgtgc ggtaacggca agatggaaaa acacatcctg cgtgaatgtt
     9901 ttgagtcata cctgcccgca agcgtggcct ggcggcagaa agagcagttc tccgatggcg
     9961 tcggttacag ttggatcgac accctgaaag aagtggcggc gcagcaggtt tctgatcaac
    10021 agctggaaac tgcccgcttc cgcttcccgt acaacacacc gacctcaaaa gaagcgtatc
    10081 tgtaccggga gatctttgaa gaactattcc cgcttccgag cgccgctgag tgcgtgccgg
    10141 gcggtccttc cgtcgcgtgt tcttccgcta aagcgatcga atgggatgaa gcgttcaaga
    10201 aaatggacga tccgtctggt cgcgcggttg gtgttcacca gtcggcgtat aagtaagaaa
    10261 tgcgaaacag ccccggtgaa atccggggct gtttcagtta ttgaggtgct tgttgattct
    10321 gatatgcagt aaggaaatgt tccgggtttg ccacatcact gtttgacaac tcatcactgt
    10381 aggccagtat ttcatagatt aatttcccac cactattgtt aaattccgaa tttcatcctg
    10441 tatgacaaaa gaatggctgt agggtgggaa aaaacacaaa gtaaacaata attgacgaat
    10501 atagcgccac gctgttcgca acctaaccaa acagtcactt tcgagcaatt ttccttgaaa
    10561 aagaggttga cgctgcaagg ctctatacgc ataatgcgcc ccgcaacgcc gataaggtat
    10621 cgcgaaaaaa aagatggcta cgtagctcag ttggttagag cacatcactc ataatgatgg
    10681 ggtcacaggt tcgaatcccg tcgtagccac catctttttt ttgcgggagt ggcgaaattg
    10741 gtagacgcac cagatttagg ttctggcgcc gcaaggtgtg cgagttcaag tctcgcctcc
    10801 cgcaccattc accagaaagc gttgtacgga tggggtatcg ccaagcggta aggcaccggt
    10861 ttttgatacc ggcattccct ggttcgaatc caggtacccc agccatcttc tttgagtaag
    10921 cggttcaccg cccggttatt ggggtatcgc caagcggtaa ggcaccggtt tttgataccg
    10981 gcattccctg gttcgaatcc aggtacccca gccatcgaag aaacaatctg gctacgtagc
    11041 tcagttggtt agagcacatc actcataatg atggggtcac aggttcgaat cccgtcgtag
    11101 ccaccaaatt ctgaatgtgt cgaatacgtt cggcaaattc aaaaaccaat ttgttggggt
    11161 atcgccaagc ggtaaggcac cggattctga ttccggcatt ccgaggttcg aatcctcgta
    11221 ccccagccaa tttattcaag acgcttacct tgtaagtgca cccagttggg gtatcgccaa
    11281 gcggtaaggc accggattct gattccggca ttccgaggtt cgaatcctcg taccccagcc
    11341 acattaaaaa agctcgcttc ggcgagcttt ttgcttttct gcgttcactc aatgtcgaat
    11401 gcgatgttga cacgtcttat ccttcaatgt cggatgcgac gctgccgcgt cttatccgac
    11461 ctacggttgg cacgcatccg gcaatgttgt aaggctacaa ccctaacgca tatttcagcg
    11521 cctgacgttt caacacgcca gcacgctccg ccgccatcaa accaagatta cgcacaaaac
    11581 gcagcggcgg cagattatta ctgaatccgg cataaaacag atccataccg ctctgcataa
    11641 tgaagttatc cgccatgcgc cgcatctggt aacgcttgag gacaggataa ctggcccacg
    11701 cttcgccgta gctgcgggca ttcaccagaa catcaatcag ggcatcgaca tcacgataac
    11761 caagattcac cccctgcccc gccagcggat ggatggtgtg cgcggcatca cccaccagcg
    11821 caagccccgg ctgcacatac tgcaacgcat ggcggcgtgt cagcggaaac gcaccagcgg
    11881 caagcggtgt aacgtaaccc agacgcgacg ggaaatgctt cgcgatttcc acctgtaact
    11941 gcgccatatt catattctgc aactggcgaa tgcgcgccgg agagtcgtac cacaccagcg
    12001 atgcccagtt atcaaacagc ggcagaaacg cgcgcggtcc atccggggta aattgctgcc
    12061 aggtgctatc gccgggatcg ttctcgcact ggacgctaat caacatgcac gactgcgcat
    12121 actgccatgc atgaacgcca attcccgcca tctgccgcac ctgcgaattt gcgccgtcgg
    12181 caccaatcac cagcttcgcg cgaatcactt caccgccttt cagctccagc tcctgaagat
    12241 cattatctcg atgtaatgca atcagcgagg ttggcacacg taacgttact ttcggatgcg
    12301 cttccagcgc ctgccacaat gcctgttgca ggacagtgtt ttctaccata tagccaagca
    12361 acggcaactt taattcggct gcgtcaaata ccacatgcgc cgtttcccac tcccacgttt
    12421 ccagtctgcg gtaaggatgg caacgcatag cctgtactgc atcccagacc cctaaccctt
    12481 taagcaatga taccgaagcc gcgctgatcg ccgagatccg cacgtccggt tggctatcag
    12541 cgacaaacgg tgcaggttct gcgtgctcga tcaccgttac cgtaaatccg tgctgtgcca
    12601 gccccagcgc cagtgcgccg ccgaccattc ctccgccgac aatggcaatt tccgttggtt
    12661 gatttgtcat ggtcaatcat cctgttagca atatgcttta agtttacagg atttttaccc
    12721 ccaagggctg ataacgctca tactggtcac aacggcagca aagcattaca ctatgcgccc
    12781 tgcattcctg gctactattt cgcaagagca agtcgatgac caaaaaactc catattaaaa
    12841 cctggggctg tcagatgaac gagtacgatt catcgaagat ggccgatctg ctggatgcca
    12901 cccacggcta tcaactgacc gacgtggcgg aagaagcgga tgtgctgctg ctgaacacct
    12961 gctcaatccg cgagaaggct caggaaaaag tcttccatca gttgggtcgc tggaaactgt
    13021 taaaagagaa gaatccagac ctgattatcg gcgtcggtgg ctgcgtggca tcgcaagaag
    13081 gcgagcacat tcgccagcgc gcccactatg tcgatattat ttttgggccg caaacgctgc
    13141 accgtctgcc ggagatgatc aactccgtgc gcggcgaccg cagcccggtt gtagatatca
    13201 gcttcccgga aatcgagaag tttgaccgtc taccggaacc gcgcgccgaa gggccgaccg
    13261 cgtttgtctc catcatggaa ggctgcaata aatattgcac ctactgcgtg gtgccttaca
    13321 cccgtggtga agaggtaagc cgtccgtctg acgatattct gtttgagatt gcccagcttg
    13381 cggctcaggg cgtgcgtgaa gtcaacctgc tcggtcagaa cgtgaacgcc tggcgtggtg
    13441 aaaactacga cggcaccacc ggatcgtttg ccgatctgtt gcgtctggtt gctgcgatcg
    13501 acgggatcga tcgtattcgc tttaccacca gccatccgat cgaattcacc gacgatatca
    13561 tcgaagtgta tcgcgacacg ccggagctgg tgagcttcct gcatctgccg gtacagagcg
    13621 gttccgatcg cattctgaac ctgatgggac gtacccatac ggcgctggag tacaaagcga
    13681 tcatccgtaa actgcgtgcg gcgcgtccag atattcagat cagttctgac ttcatcgtcg
    13741 gcttccctgg cgaaaccacc gaagacttcg agaaaacgat gaagctgatt gccgacgtca
    13801 atttcgacat gagctacagc tttatcttct ctgcacgtcc gggtacacca gccgccgata
    13861 tggttgatga tgttccggaa gaagagaaga agcagcgtct gtatattctg caagaacgta
    13921 tcaatcagca agcgatggcg tggagccgcc ggatgctcgg caccacccag cgtattctgg
    13981 tagaaggtac atcgcgtaag agcatcatgg agctttccgg gcgtacggaa aataaccgcg
    14041 tggtcaactt cgaaggcacg ccggatatga tcggtaaatt cgtcgatgta gaaattaccg
    14101 acgtctaccc gaactctctg cgcggtaaag tggtgcgtac tgaagatgaa atgggtctgc
    14161 gcgtggcaga aacaccagaa tcggtgattg cccgtactcg caaagaaaac gaccttggcg
    14221 tgggttatta tcagccgtaa ttctcaggcc cgccgttccg gtgggccttg ttttttctct
    14281 ctttatcccc atctttatcg caatgcttgc gcctttttcc tgtggcgtga ataatttcct
    14341 tataggccgg tgaatgttca gcttccggcg gcatacagat aagaggaacg gtttgaacat
    14401 agacactcgc gaaatcaccc tggagccagc agacaacgcg cgtctgttga gcctgtgcgg
    14461 cccgtttgat gacaacatca agcagctcga acgccgtctc ggcatcgaga tcaatcgccg
    14521 cgataaccac tttaaactga ccggccgtcc gatttgcgtc accgctgcgg cagacattct
    14581 gcgtagcctg tatgtcgata ctgccccgat gcgcggtcag attcaggata tcgaaccgga
    14641 acagatccac cttgcgatta aagaagcacg ggtactggag caaagcgcgg agagcgtgcc
    14701 ggagtacggc aaagcggtca atatcaaaac caaacgcggc gtaattaagc cgcgcacgcc
    14761 aaaccaggcg cagtacatcg ccaatattct cgaccatgac attaccttcg gcgttggccc
    14821 ggcgggtacg ggtaaaacct acctggcagt ggctgcggca gttgatgccc tggagcgtca
    14881 ggaaattcgc cgtattctgc tgactcgtcc ggcagtagaa gccggtgaga aactgggctt
    14941 cctgcctggc gatttaagcc agaaagtaga cccgtatctg cgcccgctgt acgacgcgct
    15001 gtttgaaatg ctgggttttg agaaagtcga gaaactgatt gagcgcaacg ttattgaagt
    15061 cgcaccgctg gcctatatgc gtggtcgtac gctgaacgac gcatttatca ttctcgatga
    15121 gagccagaac actaccatcg aacagatgaa gatgttcctg acccgtatcg gttttaactc
    15181 aaaagcggtt atcaccggcg acgtcacaca gatcgacctg ccgcgtaata ctaaatcagg
    15241 cttacgtcat gccatcgaag tgctggccga tgtcgaagag atcagcttta acttcttcca
    15301 cagcgaagac gtggttcgtc acccggtggt ggcgcgtatc gttaacgcct atgaagcctg
    15361 ggaagaagcc gaacaaaaac gtaaagcggc gctggcggca gaacgcaagc gcgaagaaca
    15421 ggaacaaaaa tgagtcaggt gatcctcgat ttacaactgg catgtgaaga taactccgga
    15481 ttgccggaag agagccagtt tcaaacatgg ctgaatgcgg tgatcccgca gtttcaggaa
    15541 gagtcggaag tgacgattcg cgtggtcgat accgccgaaa gccacagtct caatctgacc
    15601 tatcgcggta aggataagcc gaccaacgtg ctctccttcc cgtttgaagt gccgcctggc
    15661 atggagatgt cgctactggg cgatctggtt atctgccgtc agatggttga gaaggaagct
    15721 caggagcaag gcaaaccact ggaggcgcac tgggcgcata tggtggtgca cggcagtctg
    15781 catttgttag gttacgatca catcgaagat gacgaagcag aagaaatgga agccctcgaa
    15841 acagagatta tgcttgctct gggctatgag gatccgtaca ttgccgagaa agaataattt
    15901 gccagccatt tgactggcag acgtccccgc cgttggtgat taacgacggc gttttgatta
    15961 actaacatga gaacccatac gacgccatga gcgacgacaa ttcacacagt agtgacacga
    16021 taagcaacaa gaagggattt ttctccctgt tactcagcca acttttccac ggtgaaccga
    16081 aaaaccgtga cgaactgctg gcgctgatcc gtgattccgg gcagaacgac cttatcgacg
    16141 aagatacgcg cgatatgctc gaaggggtga tggacatcgc agaccaacgc gtccgcgaca
    16201 tcatgatccc ccgctcccag atgattaccc tgaaacgcaa ccagacgctg gacgaatgcc
    16261 ttgatgtcat catcgagtcc gcccactcac gtttcccggt gattagcgaa gacaaagatc
    16321 acattgaagg gattctgatg gcgaaagact tgctgccgtt tatgcgcagc gatgctgaag
    16381 ccttcagcat ggacaaagtg ttacgtcagg cggttgtcgt tcctgaaagt aagcgcgtag
    16441 accggatgct gaaagagttt cgctctcagc gttaccacat ggcgatcgtt attgacgaat
    16501 tcggtggggt ttccggtctg gtaaccattg aagacatcct ggaactgatt gttggtgaga
    16561 ttgaagacga atatgacgaa gaagatgata tcgacttccg tcagctgagt cgtcatacct
    16621 ggaccgtgcg cgcactggct tccattgaag acttcaacga agcgttcggc acccacttta
    16681 gcgatgaaga agtcgacact atcggtggtc tggtgatgca ggcatttggg catcttccgg
    16741 cacgtggcga aaccatcgac atcgacggtt accagttcaa agtggcgatg gccgacagtc
    16801 ggcgtattat tcaggttcat gtcaaaatcc cggatgactc accccagccg aagctggatg
    16861 aataaaaccg aaactggata gataactaca tggcttttgc ctcattaatt gaacgccagc
    16921 gcattcgcct gctgctggcg ttattattcg gtgcctgcgg aacgctggcc ttctctcctt
    16981 acgacgtctg gcctgcggcg attatttcgc tgatggggct tcaggcgttg acctttaacc
    17041 gccgtccact ccagtctgcc gctattggct tttgctgggg atttggcctc tttggcagcg
    17101 gtattaactg ggtctatgtc agcatcgcga cctttggcgg aatgcctggc ccggttaaca
    17161 tcttcctggt ggtactgctg gcggcgtatt tgtcgctgta taccggactg tttgctggcg
    17221 tgctgtcgcg cctgtggccg aaaaccacct ggctgcgcgt ggcgattgcc gcccctgctc
    17281 tctggcaagt gaccgagttt ctgcgcggtt gggtactgac aggcttcccg tggttacagt
    17341 tcggctatag ccagattgat ggcccgttaa aagggctggc accgctaatg ggcgtggaag
    17401 ccattaactt cctgcttatg atggttagcg gcctgctggc actggcgtta gtcaaacgca
    17461 actggcgtcc gctggtggtg gccgtcgtgc tgtttgccct tcccttcccg ctgcgttaca
    17521 tccggtggtt taccccacaa ccggagaaaa ccattcaggt ttcgatggtt cagggcgata
    17581 ttccgcaatc gctgaaatgg gacgaaggcc agcttcttaa tacgctgaag atttactaca
    17641 acgcaacggc accgctgatg ggcaaatcat cgttgattat ctggccggag tcggcgataa
    17701 ccgatctgga aattaatcag caaccgttcc tcaaagcact ggacggtgag ttgcgtgata
    17761 aaggtagctc gctggtgacc gggattgttg acgcgcgtct taataagcag aaccgttacg
    17821 atacctacaa caccatcatc acactgggta aaggtgcacc gtacagctac gaatcagccg
    17881 atcgctataa caaaaaccat ctggtgccgt ttggcgagtt tgtcccgctg gagtcgattc
    17941 tgcgtccgtt agcaccgttc tttgatctgc cgatgtcgtc gttcagccgt gggccatata
    18001 tccagccgcc gctgtcggca aatggtattg agcttactgc ggctatttgc tacgagatca
    18061 ttctcggcga gcaagtgcgc gataacttcc gcccggatac cgactatctg ctgactatct
    18121 ccaacgatgc gtggtttggt aagtctattg gtccatggca gcacttccag atggcgcgaa
    18181 tgcgtgcgct ggagctggcg cgcccactgt tgcgcagcac caacaacggc attacggcgg
    18241 tgattggccc gcagggtgag attcaggcga tgatcccgca gttcacccgc gaggtgttaa
    18301 ccactaacgt gacgccgacc accggactca caccatacgc acgtaccggc aactggccgc
    18361 tgtgggtgct gacggcactg tttggttttg ctgctgtgtt gatgagtctg cgtcagcgac
    18421 gtaaataatc ccttcttatc gtgccggaat acatcccata tttcggcacg ctcaccggat
    18481 gcggcatcag tgcattatcc ggataacaat attctcctat ccaactttgg cacatctatt
    18541 gctttgttat acaaggcaaa gctttaacca gcatcagtac aacccggttg caccacagca
    18601 atccaccggt agcaccaaaa gagtgcattt taagaatttt gcttcggaat ggtgcggcgc
    18661 acctcccaaa tataaacaat aaaacagcaa tatctttaca tttagccaaa ctaaatgtta
    18721 cctaacaggc acaacactac acaataaagt tgcagatgat aacaacacaa acactcacaa
    18781 cgggtatcca tgcgttctta acgcagaaga taaaggagtt ggatatgcaa ttacgtaaac
    18841 ctgccacagc aatcctggcc ctggcgcttt ccgcaggact ggcacaggca gatgacgccg
    18901 ccccggcagc gggcagtacg ctggacaaaa tcgccaaaaa cggcgtgatt gtcgtcggtc
    18961 accgtgaatc ttcagtacct ttctcttatt acgacaatca gcaaaaagtg gtgggttatt
    19021 cgcaggatta ctccaacgcc attgttgaag cagtgaaaaa gaaactcaac aaaccggact
    19081 tgcaggtgaa actgattccg attacctcac aaaaccgtat tccgctgctg caaaacggca
    19141 cttttgattt tgaatgtggt tctaccacca acaacgtcga acgccaaaaa caggcggctt
    19201 tctctgacac tattttcgtg gtcggtacgc gcctgttgac caaaaagggc ggcgatatca
    19261 aagattttgc cgacctgaaa ggcaaagccg tagtcgtcac ttccggcact acctctgaag
    19321 ttttgctcaa caaactgaat gaagagcaaa aaatgaatat gcgcatcatc agcgccaaag
    19381 atcacggtga ctctttccgt accctggaaa gcggtcgtgc cgttgccttt atgatggatg
    19441 atgctctgct ggccggtgaa cgtgcgaaag cgaagaaacc agacaactgg gatatcgtcg
    19501 gcaagccgca gtctcaggaa gcctatggtt gcatgttgcg taaagacgat ccgcagttca
    19561 aaaagctgat ggatgacact atcgctcagg tgcagacctc cggtgaagcg gaaaaatggt
    19621 ttgataaatg gttcaaaaat cccattccgc cgaaaaacct gaacatgaat ttcgaactgt
    19681 cagacgaaat gaaagcactg ttcaaagaac cgaatgacaa agcactgaac taattacaag
    19741 aaccaggggc ggaaattcta gccctctcga ttgttacgta gcacggacag actatacgcc
    19801 tgatggtcgt tccccatcgg gcctgaaaac cgcaatacgc tgggtaacaa tcttcgaggg
    19861 tagcagttaa cgctgctacc ctcttttttc tggagtagat ttatgtctat agactggaac
    19921 tggggtattt ttttacaaca agccccgttc ggcaacacca cctatcttgg ttggatctgg
    19981 agtggttttc aggtgacgat cgctttgtcg atctgcgcct ggattatcgc tttcctcgtc
    20041 ggttcatttt tcggtatttt acgtaccgtt ccaaaccgtt ttctctccgg tctgggtacg
    20101 ttgtatgtcg aactgttccg caacgtgccg ttgattgtgc aattctttac ctggtatctg
    20161 gtgatcccgg agctgctgcc ggagaaaatc ggcatgtggt ttaaggccga gctggatccc
    20221 aatattcagt ttttcctttc atccatgctc tgcctggggc tgtttaccgc cgcccgtgtt
    20281 tgcgaacagg tacgcgccgc gattcagtcg ctgccgcgtg ggcagaagaa tgccgcgctg
    20341 gctatgggtc tgacgctgcc gcaggcttac cgctacgtac tgctgcctaa tgcttatcgc
    20401 gttatcgtcc cgccgatgac ctcagagatg atgaacctgg tgaaaaactc cgccatcgcc
    20461 tcgaccatcg gtctggtaga tatggcagcg caggcgagta aattgctcga ttactcggct
    20521 cacgcctggg agtcgtttac cgccattacg ctagcgtatg tgtttattaa cgcttttatc
    20581 atgctggtga tgacgctggt tgaacgtaaa gttcgcctgc ctggcaacat ggggggcaaa
    20641 taatgtacga gtttgactgg agttccattg tcccttccct gccatatctg ctcgacgggc
    20701 tggtgatcac gctgaaaatc accgtcacgg cagtcgtgat tggtattttg tggggcacca
    20761 tgctggcggt gatgcgcttg tccagctttg cgcccgtagc gtggtttgcc aaagcctacg
    20821 ttaacgtatt ccgctcgatt cctttagtta tggttttgct gtggttttac ctgatcgtgc
    20881 cgggttttct gcaaaacgtg ctgggattat caccaaaaaa tgatatccgt ttaatttcgg
    20941 cgatggtggc gttttcgatg tttgaagcgg cctactattc agagattatc cgtgccggta
    21001 ttcaaagtat ttctcgtggt cagtcgagcg ccgcgctggc gttgggaatg actcactggc
    21061 agtcgatgaa actgattatt ctgccgcagg cgttccgcgc gatggtgccg ctgctgctca
    21121 ctcagggcat cgtactgttc caggatacct cgctggtgta tgtgttatct ctggccgatt
    21181 tcttccgtac cgcctcaacc attggtgagc gtgatggcac gcaggttgag atgatcctgt
    21241 ttgccggatt tgtttatttc gttattagcc ttagcgcgtc gttgttggtc agctacttga
    21301 aaagaaggac agcataatga ttaccctgaa aaatgtttca aaatggtatg gtcactttca
    21361 ggtgctgacc gactgctcaa ccgaagtgaa aaaaggcgaa gtggtggtgg tttgcggccc
    21421 gtctggttcc ggcaaatcaa cgctgattaa aaccgtcaac ggcctcgaac cagtgcagca
    21481 aggtgaaatc accgtcgatg gtatcgtggt taacgacaag aaaaccgatc tggcaaagct
    21541 gcgttctcgc gtcgggatgg tattccaaca tttcgagctg ttccctcatc tgtcgattat
    21601 cgaaaacctg accctggcgc aggtgaaagt gcttaaacgc gataaagcac cggctcgtga
    21661 aaaagcattg aaattgctgg agcgcgtggg actttccgca catgccaata aatttccggc
    21721 gcagctttcc ggcggccagc agcagcgtgt ggcaattgct cgcgcattgt gtatggatcc
    21781 tattgcgatg ctgttcgacg aacccacatc ggcactagac ccggagatga tcaacgaagt
    21841 actggacgtg atggtggaac tggcgaacga ggggatgacc atgatggtgg tgacccacga
    21901 aatgggcttt gcccgtaaag tggcgaatcg ggtgatcttt atggatgagg gtaaaattgt
    21961 tgaagactcg ccgaaagatg cgttcttcga tgatccgaaa tcggaccgcg caaaagactt
    22021 cctcgcgaaa atcctgcatt aatcactctg gcgcgcatac ccaagtgcgc gccatctcac
    22081 gctttatcgt ctcgcccctc gtatctgtta catcctggcg ttacccttgt cgcaaagaag
    22141 cacacaacaa ggagcaacaa tggcactgcc aattctgtta gattgcgacc caggtcatga
    22201 tgacgctatc gcaatagttc tcgccctcgc ctcaccagag cttgatgtca aagcaattac
    22261 gtcttccgcc ggaaaccaga caccagaaaa aaccttacgc aatgttctgc gtatgctgac
    22321 cttgcttaat cgcaccgata ttccggtggc aggcggcgcg gtaaaaccgt taatgcgtga
    22381 gttgattatc gcggacaatg tgcacggcga aagcggtctc gacggcccgg cattaccgga
    22441 accgacgttc gcaccgcaaa actgtacggc ggtagagctg atggcgaaaa cgctgcgtga
    22501 aagtgcggaa cctgtcacga ttgtgtctac cggaccgcaa actaacgttg ccttgctgct
    22561 caatagccat ccggaactgc atagcaaaat tgcccgtatc gtgattatgg gtggcgctat
    22621 gggactgggt aactggacgc ctgcggctga atttaatatt tacgttgacc cggaagcggc
    22681 agaaattgtc ttccagtcag ggatcccggt ggtgatggcc ggtctggatg ttactcacaa
    22741 agcacaaatc cacgttgaag acactgagcg tttccgcgcg attggcaacc ctgtttcaac
    22801 cattgttgcc gaactgctgg atttcttcct cgaatatcat aaagacgaaa aatggggctt
    22861 tgtcggcgca ccgctacatg acccatgcac catcgcctgg ctgttaaaac cggagttgtt
    22921 taccactgtt gagcgctggg ttggcgtgga aacacagggg aaatataccc agggtatgac
    22981 ggttgttgat tattattatc tgactggcaa taaaccgaat gccactgtaa tggtcgatgt
    23041 tgatcgtcag ggctttgttg atttactggc tgatcgtctg aaattttacg cttaacacac
    23101 gtcattgatt aagatcacca cgcgttacct gggtggtctt aatctgttta tctctttttc
    23161 tttcaagata taaatgcaat ggataatgca gaactcgcca ttggtatcga tctcggtact
    23221 accaatagtt taattgccgt ctggaaagac ggtgccgcgc aattaattcc aaataagttc
    23281 ggtgaatatt taacaccatc cataattagc atggatgaaa ataatcatat tttagtcgga
    23341 aaaccggctg tatcacggcg tacttcgcat ccggataaaa cggcagcgtt atttaaacgt
    23401 gcaatgggca gtaataccaa ctggcggtta ggcagcgaca catttaacgc gccagaactg
    23461 tcctctttgg tattacgctc attaaaagaa gatgccgaag aatttctgca acgtccgatt
    23521 aaagatgtgg tgatctctgt tccggcttat ttcagcgatg aacaacgcaa gcatacccgt
    23581 ttagcagcgg agttagccgg tttaaatgcg gtacgcttaa ttaatgaacc cacagcagct
    23641 gcgatggcgt atggcctgca tacccaacaa aatacccgtt cgctggtgtt tgatctcggt
    23701 ggcggcacgt ttgacgtcac ggtgcttgag tacgccacgc cggtgattga agttcacgcc
    23761 tccgctggcg acaacttcct cggtggcgaa gattttaccc atatgctggt cgatgaggtt
    23821 ttaaaacgcg cggatgtcgc caggaccacg cttaacgaga gtgaactggc agccttgtat
    23881 gcctgtgtgg aagcggcaaa atgtagcaat caatcgccaa tacacattcg ctggcagtac
    23941 caggatgaaa cgcgggaatg cgaattttac gagaacgaac tggaagattt gtggttgccg
    24001 ctgctcaatc gcttgcgagt gccgattgaa caggcgttgc gcgatgcgcg tctgaagccg
    24061 agtcaaatcg acagtctggt gctggttggc ggcgcgtcac aaatgccgct ggtgcagcga
    24121 atcgccgtgc gtctgtttgg caaattaccg tatcaaagtt acgatccgag caccattgtc
    24181 gcgctgggcg cagcaatcca ggcagcctgc cgcttacgca gtgaagatat tgaagaagtg
    24241 atcctcactg atatttgccc ttactcgttg ggcgttgagg ttaaccgcca gggcgtttcc
    24301 ggcattttct cgccgattat tgaacgaaac accactgtgc ccgtgtcgcg tgtagaaact
    24361 tattcaacca tgcacccgga acaggattct attacggtta acgtctatca gggagaaaac
    24421 cacaaagtta aaaacaacat tctggtggaa tccttcgatg tgccgttgaa gagaaccggg
    24481 gcttatcagt cgattgatat tcgctttagt tatgatatca acggtttgct tgaagttgac
    24541 gtgcttctgg aagacggcag cgttaagtcc agagtgatta accacagccc ggtaacattg
    24601 agcgcgcagc agattgaaga gagtcggacg cggttatccg cattgaaaat ttatccgcgc
    24661 gatatgctca tcaaccgcac ctttaaggcc aaactggaag agttgtgggc gcgggcgctg
    24721 ggtgacgagc gagaagagat tggccgggtg atcaccgatt ttgatgcggc gctgcagtca
    24781 aacgatatgg cccgcgtcga tgaggtcagg cggcaggcga gcgtttattt agccattgag
    24841 atcccataat cacggctgcc ggatgcggcg taaatgctgc atccggcatc ggcgcaaaaa
    24901 acacatcgtc aactaaacaa catcgcgatt ttgccgagag cataacctat caccagcacc
    24961 gttaagctga ttttgataat gctggtaatg ctctgtggtt tttcatcctc atcatttttt
    25021 tcttcatggt tttcaggtgt tggctcatcg acatcttcat cctccggctc gagttttact
    25081 ttcgtcaaca gcatccgggt gataatggcc tctaatggat gagcatctcc gccttccagc
    25141 cttgtattaa gacgggccag catctcttgt ttcggaaacg cttcaagtac tacctgttcc
    25201 gctacccgat acaaatccag taacggtgaa tcttcttctg tctcgctacc gactctcgat
    25261 ttccaccacg cccatgcttt gcgtttgaag aatagcgact caataaacga ttgacctaac
    25321 ggcgatagtt ttccgctatg taaaatttcc gcccataaca gacgcgtccg ggcgctctca
    25381 ccaggaacat atttcaacgg tttaccttgc gcatctacca tgctccgcgc ttcgataacc
    25441 atcactaata acgcgaaata atccgggcaa tgttgtgcgc tccactgcaa caacaaatta
    25501 tcggcttgtg tagatgggta acttccccag tacgcgcaga gatcggtaag caggctttcc
    25561 ccctcgccgc agtacaaacg ttgtgcacac tggtaatacc gggcatcttc gctttctggt
    25621 tcttccattt gccactgttc ggctacagga atgagttccg ccatacctgt ttgcaccgag
    25681 ctgtaccagc gcaacagttt gcgatgtaac tttgcatcat caggaatgaa ccacggacct
    25741 tccatcgcca aaaacgcagt gacatagtca ggatggtaac ggcagcaacg ttccagcgcg
    25801 aagtaatact caacagtctg gttctgcaac gcaacgggat ataccagaag gccaaggaaa
    25861 tcgaaacaat caccagcctg aatggcctca agaaaagcct ccctttctta ctcatctatt
    25921 ccttcgctgt cattaaacga atgccagttc aaacgctctg ccagcaaaga agcacaattt
    25981 aatgaaagat atcgcgcttc tatagcgatt gcacacagcg gccagcgtaa ttgatcgaca
    26041 tcctccatgt tccaggtatt taattgctga ataaatttct gccaggcgga gggctgaaaa
    26101 cgatcacttt ctgaatccag taatgccctg aatacacgta gtatttcatg ctcagtggca
    26161 tcatcttttt cttcatcatc tgcttcctcg acaggattta ccgccagtcg cagggcctct
    26221 tcataggcct ggcgtaatgc tttaaaccct tgcggatcgg tttcgggatg acataacggt
    26281 aagcgagcaa gataagcctg gcggataatg tctatttgcg tcgtgctttc aatttcgagg
    26341 atttgccaac atgttttcat aggtcgctca catccttaag cgttaccggg cattcaggct
    26401 ggacagttaa tggctgccag ggaagcgtgg catagaaacg atcgcttccc gcagccagct
    26461 cttcgaacat ccgactcccg gcgctatcgt atttgtacag ggttagcgcc tcgcgtagcg
    26521 aagcgttgtc ttcgcattga gaagattgcc agtagagacg ccctagtgaa taggcggaaa
    26581 aatagtgcat ccagctgtga tagtaatggt gagctcgttc atagacgcgc gattgcagcc
    26641 ataaactctc gtcttccgtc agccacttgt tgagcacacc gacccgtgac agaaacccca
    26701 ttcgaacata atcccaagct ttgattcccc cttccccaca acacatagcg gtttgggcaa
    26761 cgaattgggt ataaatcaac cctctctccg agccaccagc aattaatgct ttccactctt
    26821 caggggaaga gcgatgccat gtcagatata agccagctaa atcattggca tgaccatcat
    26881 cagtcatctg ataaatagtc tgaattaaat catcacgatt atttattccc caggatgatg
    26941 agagatcgaa ttcttttatc ccggtataca tttttggcga gtcatgcgcg ggtttgcaac
    27001 cctgattaag tatttccatg g
//
LOCUS       DAZKIP010000055        26187 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZKIP010000055 DAZKIP010000000
VERSION     DAZKIP010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26187
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.055"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(160..1023)
                     /gene="potB"
                     /locus_tag="ACHOUK_002546"
     CDS             complement(160..1023)
                     /gene="potB"
                     /locus_tag="ACHOUK_002546"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFP9897967.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLARDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKATEIDN"
     gene            complement(1007..2143)
                     /gene="potA"
                     /locus_tag="ACHOUK_002547"
     CDS             complement(1007..2143)
                     /gene="potA"
                     /locus_tag="ACHOUK_002547"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFP9897968.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            2393..3619
                     /gene="pepT"
                     /locus_tag="ACHOUK_002548"
     CDS             2393..3619
                     /gene="pepT"
                     /locus_tag="ACHOUK_002548"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFP9897969.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            complement(3668..4789)
                     /gene="roxA"
                     /locus_tag="ACHOUK_002549"
                     /gene_synonym="ycfD"
     CDS             complement(3668..4789)
                     /gene="roxA"
                     /locus_tag="ACHOUK_002549"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFP9897970.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYTNGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            5038..6267
                     /locus_tag="ACHOUK_002550"
     CDS             5038..6267
                     /locus_tag="ACHOUK_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9897971.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFITQLVEQNRHTGLLLGEVKQEASVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            6632..6820
                     /locus_tag="ACHOUK_002551"
     CDS             6632..6820
                     /locus_tag="ACHOUK_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9897972.1"
                     /translation="MLSTDRFIREKECEKLTGLSRTCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            6878..7432
                     /locus_tag="ACHOUK_002552"
                     /pseudo
     CDS             6878..7432
                     /locus_tag="ACHOUK_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
     gene            7625..7822
                     /locus_tag="ACHOUK_002553"
     CDS             7625..7822
                     /locus_tag="ACHOUK_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFP9897973.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARN
                     RCPSGCVAVFAARIRQEVRHD"
     gene            7815..8027
                     /locus_tag="ACHOUK_002554"
     CDS             7815..8027
                     /locus_tag="ACHOUK_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9897974.1"
                     /translation="MINLSLTDLNRIQFREKFTGQLLVNVDKGRVVCNYHLPDEAIVA
                     TRESLQEITKRAGMINTNIQGMLYAQ"
     gene            8017..8481
                     /locus_tag="ACHOUK_002555"
     CDS             8017..8481
                     /locus_tag="ACHOUK_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9897975.1"
                     /translation="MHNKTTPDAAAEAIKTLMHALIDISVIADRAHKHATSETEYAGA
                     FVPHSLAVMQFSADTALNEAKAILIADCENGGVMRDDRFNSLKQEFSGVPDDAADALS
                     SMPELIRAAFFLLSTREYKSTGLDVLNIAADYAEYVAEARYRRKFPEDVSHA"
     gene            8474..8707
                     /locus_tag="ACHOUK_002556"
     CDS             8474..8707
                     /locus_tag="ACHOUK_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9897976.1"
                     /translation="MRDIYHQTIDRAFLALSHSENMMEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            8713..9012
                     /locus_tag="ACHOUK_002557"
     CDS             8713..9012
                     /locus_tag="ACHOUK_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP9897977.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVEERRVTY
                     RREGYEYDCVMPVYQFRRDFSLVQTAPHNVPTSNARARANIQKLKTMVNGFRGKK"
     gene            9009..10409
                     /locus_tag="ACHOUK_002558"
     CDS             9009..10409
                     /locus_tag="ACHOUK_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HFP9897978.1"
                     /translation="MKSAPNLKKQPYDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVVLADDQLDELAELRIIDEGRYCVRLYKAGHIRPSNINAIAHKLAAAGVTDANYY
                     PEGMHSHMRENWREYLERVRGKEPAEEKNHQRKTTLPMSVGSTGYDTQLDYVVKGIIP
                     AVSLCSIYGASGSYKSFLAGSWACHVATGRQWGGRRVAHGAVLYVVGEGGIGVPRRVK
                     AWEVVHDEQVKNLYLVNRPIFPAAPLDVDEMVIAARQVERETGKPVRMIILDTLARCF
                     GGNDENDSRDMGAFIRGCDELKRRTGATVLVVHHSGKDETKGARGSSAFRASLDAEYR
                     IRREDAGSEALVISCTKMKDAEELKEAAYDLRVVELFTDADGELITSLVVVDDPRPPV
                     ELERIEEAGNKTENHTALWGCIRSRTQNGDKCTIPLLRDDMKKLGYEMKNFRRWLYKL
                     EKDGVIRIDGDDVAPL"
     gene            10610..10861
                     /locus_tag="ACHOUK_002559"
     CDS             10610..10861
                     /locus_tag="ACHOUK_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9897979.1"
                     /translation="MTQKRRDRTEPKYKALDMTEHTLKVAIRTIDRHTREGYAKEHPD
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKTGEVEL"
     gene            10858..11268
                     /locus_tag="ACHOUK_002560"
     CDS             10858..11268
                     /locus_tag="ACHOUK_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP9897980.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVIAFGKQAGFLAKHQKGDVASVSGTMQVSQWTGQNGETRQGWQVIADSVISA
                     RAARPGGNRRKTTGTQGNQPPAGGDDPYGDDISF"
     gene            11279..11551
                     /locus_tag="ACHOUK_002561"
     CDS             11279..11551
                     /locus_tag="ACHOUK_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP9897981.1"
                     /translation="MVHDRIAEELEAKGFYRRAAARWGEVMQLVETDKERHHITMRRL
                     ECSRKAQRAPEPPDNFGDLKKAVDRTYAEMGIDGAGDEIWRNYQDS"
     gene            11508..11636
                     /locus_tag="ACHOUK_002562"
     CDS             11508..11636
                     /locus_tag="ACHOUK_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001301569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFP9897982.1"
                     /translation="MVLVMKYGAITRTANQTAGEIRLFLHQLKRYGALPGFRHGQA"
     gene            11678..11902
                     /locus_tag="ACHOUK_002563"
     CDS             11678..11902
                     /locus_tag="ACHOUK_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9897983.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMSINDYRAFLETGALIEKDQHG
                     FVRCALSGEMLAVNPEQIDALIEFLKEIRD"
     gene            12154..12360
                     /locus_tag="ACHOUK_002564"
     CDS             12154..12360
                     /locus_tag="ACHOUK_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9897984.1"
                     /translation="MAEEKKSGVSVYISPEIVEVLKQRHKKNYEAGVAAGLDPLMTPE
                     PSIGSLVRSYLLAALGMHKNYGGE"
     gene            12360..13415
                     /locus_tag="ACHOUK_002565"
     CDS             12360..13415
                     /locus_tag="ACHOUK_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFP9897985.1"
                     /translation="MAGKATALNTNQLFMYLNRGDIADFKFSPLFTTLFFPNVATFST
                     QNIMLDTLDIEEVTMSAFCSPMVGSQVQRDKGYETSTIKPGYMKPKHEIDPTKTIMRM
                     AGEDPAQLNDPTYRRMRLITGNMRRQINAIKARVEWLAVNAVTTGKNIIEGEGIERYE
                     IDWKIPENCIIEQAKGKKWSEQDKDMHDPIYDIELYADQAGCPANVMIMGAEVWRTLR
                     SFKKFRELYDLSRGSESAAELACKNLGEVVSFKGYLGDLALIVYSGKYTDSDGTEKYF
                     LEPDLLVLGNTNNKGLVAYGAIMDQEAVRTGATQNMFYPKNWIEDGDPAIEYVQTHSA
                     PQPVPADIRKFVTVKIG"
     gene            13428..13763
                     /locus_tag="ACHOUK_002566"
     CDS             13428..13763
                     /locus_tag="ACHOUK_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFP9897986.1"
                     /translation="MDTPYIELFAGSQQVSTTLVHFAADAGVIQEFTPLMLADNGEFK
                     PWDGQESGKAVYLTSYPVDTSKQKSAQCYKTGIFNIAAVNWPESVDTDAKKCAAFAGS
                     GVSVQPLAR"
     gene            13776..14189
                     /locus_tag="ACHOUK_002567"
     CDS             13776..14189
                     /locus_tag="ACHOUK_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HFP9897987.1"
                     /translation="MATNESIMTLPLASKFKVEARAMADRGLSTYEAVYQLNKLEEQD
                     KPCADAIMALHEHKDYQPLLRAMANVPCIDVGTAKSILSMTIEQERPKVAPELTAAFE
                     NFMDMHSPQAVSAGMAYDGRNQGDDGDIDRILKTI"
     gene            14395..14937
                     /locus_tag="ACHOUK_002568"
     CDS             14395..14937
                     /locus_tag="ACHOUK_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFP9897988.1"
                     /translation="MKSHLMNKKNMAKSCRVSATAFDKWGVTPVERKGREAFYDVASV
                     IENRVNNAISQLINDKGEIDDDELLRVRIRLLTAQAEAQELKNDRDRGDVIDTEFCLY
                     ALSKLASQISSIMDSLPLTMQRSFPQITPAMLDRLKREVVKACNASARVADNLPQILA
                     DYLKETTGNVPEKLLQKKGE"
     gene            15193..15474
                     /locus_tag="ACHOUK_002569"
     CDS             15193..15474
                     /locus_tag="ACHOUK_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9897989.1"
                     /translation="MTEAELLGLIRRVAGISQQHDEQATQPDSVTAENYVRVVAEVMR
                     RDGIELNGVDMRNIRNRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(16075..17535)
                     /gene="phoQ"
                     /locus_tag="ACHOUK_002570"
     CDS             complement(16075..17535)
                     /gene="phoQ"
                     /locus_tag="ACHOUK_002570"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFP9897990.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMHGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITKQYEGKIVAGESMLGGARMEVIFGRQHSTPKDE"
     gene            complement(17535..18206)
                     /gene="phoP"
                     /locus_tag="ACHOUK_002571"
     CDS             complement(17535..18206)
                     /gene="phoP"
                     /locus_tag="ACHOUK_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFP9897991.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLICRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(18375..19745)
                     /gene="purB"
                     /locus_tag="ACHOUK_002572"
     CDS             complement(18375..19745)
                     /gene="purB"
                     /locus_tag="ACHOUK_002572"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFP9897992.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(19749..20390)
                     /gene="hflD"
                     /locus_tag="ACHOUK_002573"
     CDS             complement(19749..20390)
                     /gene="hflD"
                     /locus_tag="ACHOUK_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFP9897993.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLKRKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(20426..21532)
                     /gene="mnmA"
                     /locus_tag="ACHOUK_002574"
                     /gene_synonym="trmU"
     CDS             complement(20426..21532)
                     /gene="mnmA"
                     /locus_tag="ACHOUK_002574"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFP9897994.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILIVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPI"
     gene            complement(21586..22047)
                     /gene="nudJ"
                     /locus_tag="ACHOUK_002575"
     CDS             complement(21586..22047)
                     /gene="nudJ"
                     /locus_tag="ACHOUK_002575"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFP9897995.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(22057..22710)
                     /gene="rluE"
                     /locus_tag="ACHOUK_002576"
     CDS             complement(22057..22710)
                     /gene="rluE"
                     /locus_tag="ACHOUK_002576"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFP9897996.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREATE"
     gene            22882..24132
                     /gene="icd"
                     /locus_tag="ACHOUK_002577"
     CDS             22882..24132
                     /gene="icd"
                     /locus_tag="ACHOUK_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFP9897997.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYELAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            complement(24246..25388)
                     /locus_tag="ACHOUK_002578"
     CDS             complement(24246..25388)
                     /locus_tag="ACHOUK_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFP9897998.1"
                     /translation="MAASPRSHKISIPNLYCKLDKRTGKVYWQYKHPLSGRFHSLGTD
                     ENEAKQVATEANTIIAEQRTRQILSVNERLERMKGRRSDITVTEWLDKYNSIQEDRLQ
                     HNELRPNSYRQKGKPIRLFREHCGMQHLKDITALDIAEIIDAVKAEGHNRMAQVVRMV
                     LIDVFKEAQHAGHVPPGFNPAQATKQPRNRVNRQRLSLPEWQAIFDSVSRRQPYLKCG
                     MLLALVTGQRLSDICNLKFSDIWDDMLHITQEKTGSKLAIPLNLKCDALNITLREVIS
                     QCRDAVVSKYLVHYRHTTSQANRGDQVSANTLTTAFKKAREKCGIKWEQGTAPTFHEQ
                     RSLSERLYREQGLDTQKLLGHKSRKMTDRYNDDRGKDWIIVDIKTA"
     gene            complement(25378..25614)
                     /locus_tag="ACHOUK_002579"
     CDS             complement(25378..25614)
                     /locus_tag="ACHOUK_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFP9897999.1"
                     /translation="MSRLITLRDWAKEEFGDLAPSERVLKKYAQGKMMAPPAIKVGRY
                     WMIDRNSRFVGTLAEPQLPINANPKLQRIIADGC"
     gene            25754..26187
                     /locus_tag="ACHOUK_002580"
                     /pseudo
     CDS             25754..26187
                     /locus_tag="ACHOUK_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
BASE COUNT         6901 a         6209 c         6708 g         6369 t
ORIGIN      
        1 accatttgtt gtttttttat agcgatttgc taaaagggag aagaaaaaac gttctccaaa
       61 attagattgc aactgtttga ttttatggat agaggcatgc tgtttactag acagtaaaaa
      121 gcatgccaaa tttcctatta accttttgaa tataaagcac taattatcta tttctgttgc
      181 cttcttattc agcagacgag aagcgcgcca gtaaaccagc aacatcaggc ccattacgat
      241 agtcagcgta atgctggtgg ctgcaccaaa cggccagtca cgaatattga ggaactgtac
      301 cttgatgacg ttaccgatca gcaggttttt cgcaccgccc atcaggtcgg acacatagaa
      361 caggcccatc gctggcagca tcaccagcag acatccggca ataatccccg gcatcgtcag
      421 tggaataatg atgcggataa aagtctgtaa cttgctggca ccgagatcgc gcgccgcctc
      481 aagcaacggc ttatccagtt tttcgatact ggagtacagc ggcatcacca taaacggtag
      541 cagaatgtaa accagaccga taatcaccgc actgggcgtg aacatgatac gaattggtgt
      601 gtcgataacg cccagccaga gcaaaaactc gttgagatag cctttggtgc tgaggaaaat
      661 tttcagcccg tagatgcgaa tcagtgagtt ggtccagaag ggaacaatca gcagaaacag
      721 cagcagcgga cgcaccttgt gtggcagctt cgccagaaac caggcaaacg gatagcccag
      781 caccaggcag gcgagggtgg cgatcagcgc catattcagc gagtgcaata gcacttcaaa
      841 atagagcgga tcgagcagac gtgtgtagtt atccagcgta aagaccattt tgacaaaact
      901 ggcgtcgtcg cgggccaaaa agctggtgcc aatgatcatc aggttgggca gaaagacaaa
      961 caacacaagc caaccgacaa tagtgacaat cactacattc tggaacttac ttgtgttctt
     1021 catcagccag tacgacctcc cagctttcta cccaattaat ggccattttt tggtcgagag
     1081 agtggtcaaa gtcaggatcg tcttcattga agaattcgct gaccatcacc atcttgccat
     1141 tttccagttc aacaaccgac tccagcgtca tgcctttgta gttacgctcg cgaacgtaac
     1201 caatcagccc ttcagcgtgg ttgtcgtcgt taatctcttc aacacgtaag tcttccgggc
     1261 gcagcagaac atgcagtttt tgccccggtt caacggcgaa gttaacgtag atattacatt
     1321 cgcggccttc aacgttggcg cgtacgcgct gctcgtcgag acgttcgatg acagtggcgt
     1381 taaacatatt gatttcgcca atgaacccgg caacaaacag gtttttcggc tcttcgtaga
     1441 tttcacgcgg cgtgccgtct tgctcaatgc gaccatcgcg catcaccaca atcctgtctg
     1501 acatggtgag tgcttcttcc tgatcgtgag tcacgaagac gaatgtaatg ccaagcttac
     1561 gctgtaacgc tttcagctcg ttctgcattt gcttacgcag tttgtaatcc agcgctgaga
     1621 gcgactcatc cagcaacaac agacgaggct tgttaaccac cgcgcgagca atggcgacgc
     1681 gttgctgttg accaccagag agctgatgcg gtttacgttg agcgaaggtt tccagctgca
     1741 ccatccgcag ggcttccatc acgcggggcg taatttcagc agcgggggtt ttttgcatgc
     1801 gcaacccaaa ggccacattt tcgaacacgg tcatgtgggg gaaaagtgcg tagctttgga
     1861 aaacagtgtt cacatagcgg ttttccgccg gaacgtgggt gatgtcctcg ttatccaaca
     1921 tgatgcgtcc ggaatcaaca gtttccagac ctgcaatcag gcgaagaacg gttgttttac
     1981 cgcagccaga agggccaagc agcgtgagga actcgccatt gttgatagtc agatccagct
     2041 ggggaatgac ctctttacca tcaaagcatt tgcgaattcc cgccaattgc accagcggtg
     2101 aaagcgaact cggttgttta ttcaattttt tactctgtcc catgtaaacg caacggatgg
     2161 cttaccgatg cggggtttgt ggttaaccac cttggtgact cttaatgagg gcggtaattc
     2221 tacggcaaac cgcttgaatc gccaatcttt gttgtgaatt actggcttag ctttatattc
     2281 attaaggtaa tgctgataaa tattcccgct tgcaggggta aaagtgacct gacgcaatat
     2341 ttgtcttttc ttgcttctta ataatgttgt cacaaaaagt gagggtgact acatggataa
     2401 actacttgag cgatttttga actacgtgtc tctggatacc caatcaaaag caggggtgag
     2461 acaggttccc agcacggaag gccaatggaa gttattgcat ctgctgaaag agcagctcga
     2521 agagatgggg cttatcaatg tgaccttaag tgagaagggc actttgatgg cgacgttacc
     2581 ggctaacgtc cctggcgata tcccggcgat tggctttatt tctcatgtgg atacctcacc
     2641 ggattgcagc ggcaaaaatg tgaatccgca aattgttgaa aactatcgcg gtggcgatat
     2701 tgcgctgggt atcggcgatg aagttttatc accggttatg ttcccggtgc tgcatcagct
     2761 actgggtcag acgctgatta ccaccgatgg taaaaccttg ttaggtgccg atgacaaagc
     2821 aggtattgca gaaatcatga ccgcgctggc ggtattgcaa cagaaaaaca ttccgcatgg
     2881 tgatattcgc gtcgccttta ccccggatga agaagtgggc aaaggggcga aacattttga
     2941 tgttgatgcc ttcgatgccc gctgggctta cactgttgat ggtggtggcg taggcgaact
     3001 ggagtttgaa aacttcaacg ccgcatcggt caatatcaaa attgtcggta acaatgttca
     3061 tccgggcacg gcgaaaggag tgatggtaaa tgcgctgtcg ctggcggcac gtattcatgc
     3121 ggaagttccg gcggatgaaa gcccggaaat gacagaaggc tatgaaggtt tctatcacct
     3181 ggcgagcatg aaaggcaccg ttgaacgggc cgatatgcac tacatcatcc gtgatttcga
     3241 ccgtaaacag tttgaagcgc gtaaacgtaa aatgatggag atcgccaaaa aagtgggcaa
     3301 agggttacat cctgattgct acattgaatt ggtgattgaa gacagttact acaatatgcg
     3361 cgagaaagtg gttgagcatc cgcatattct cgatatcgcc cagcaggcga tgcgtgactg
     3421 cgatattgaa ccggaactga aaccgatccg cggcggtacc gacggcgcgc agttgtcgtt
     3481 tatgggatta ccgtgcccga acctgttcac tggcggttac aactatcatg gtaagcatga
     3541 gtttgtgact ctggaaggta tggaaaaagc ggtgcaggtg atcgtccgta ttgccgagtt
     3601 aacggcgcaa cggaagtaag cgaaaaggga tgcggcatgt gatgccgcat ccggcttaaa
     3661 tccaaactta cccttcgaag aaccaatacc cgctattgac cagcgccgcg agcatcgcga
     3721 ggaatgacgg atcttccagc gcatcgccaa aattctccgc agtcagcgca atgttgctgg
     3781 cgagtgcatc cagtgccgga cggtgcgggg aatcgatctt ctcaccattg gtatacacgt
     3841 cgtcgccaat gcgcaatacg cgcagaccac ccaggcgcac cagcacatca ccttgtttca
     3901 gcgcatcgta gatttcatct ggctgataag gcggctccgg cggcgcgata tccagttcat
     3961 gacgtgactg ggatataaac tcgccaaacc attgcttaaa gtgttccggc tggttgatca
     4021 attcgagcat catctcacgc agtttatcca tctcttgcgg cagaacgtcc gcaggatgag
     4081 cgcgaggtgg aacatcagga tcgctgtagt agttgccgcc cagttcgcgt tgcagcacat
     4141 aatcggcaaa tccgctaatt aattcccgcg tatttggcgc gcgaaaaccc acggaatagt
     4201 tcatcgcatt ttccagcgcg tagccttcat gcgggaatcc tggcggaata taaagaatat
     4261 cgccaggctc cagctcttca tcgatgatgg cttcgaacgg atcgacctgt aacagatccg
     4321 gatgtgggca gtgctgtttc atttgcagct tctcgcccac tcgccagcga cgacgtccgg
     4381 taccctgaat gataaacacg tcgtactgat cgagatgcgg gccgacgccg ccgccaggta
     4441 cagaaaaaga gatcatcaga tcatcaatac gccagtccgg tagttcacgg aacggtcgca
     4501 tcagcgcggc ggtcggctca tgccagtggt tcactgcttg cactaacaat gaccagttag
     4561 tttcaccgag atgatcgtag ctttcgaacg gaccgtggct gacctgccat tttccatcct
     4621 ggtgactgac cagtcgactg tcgacttcgc tttccatcgc cagacccgcc aactcgtctg
     4681 gagagatcgg gtcaataaaa ttattaaagc cgcgttttaa caccaccggg cgtttctgcc
     4741 agtgacgttc aagaaaatcg ggccagttaa gagtgagttg gtattccatg ttaagcatcc
     4801 acaggctggt atctgcaacc gattataacg gatgcttaac gtaatgcgtg aagtatggac
     4861 atattttatc aattagtgtt ttttgccgtt tcataatgtt tcagattgtt cattatattt
     4921 ttgattataa acagatttgt gattttcctt gttccagctt gtttcacgtt gtagcccttt
     4981 gtgcaacgat cggtgtagtt aatggtgtag ttaattgtga tgaccggaat caaaattatg
     5041 agcagagcac ttaacaaact gagcgataca cagctgagga aaatcaacgg cacacccgcc
     5101 caaaaaacag cctttcttaa tgacggtgga aacctgagcg tcaggcattc aaccagtggc
     5161 cttttaacct ggtatttcac ttacagggcc ggaacgggaa ggggggcacc accggaacgc
     5221 attaagctgg gaaattatcc tgatctgagc ctgaaatcag ccagggaaaa agccgcccag
     5281 tgtcgcgcat ggctggcaga ggggaaaaat ccacgtcatg agcttaatta caccgtacag
     5341 gaagcgttaa agccggtaac ggttggcgat gcgctcacct actggcttga gtcgtacgca
     5401 aaggaaaacc gcgtggatta tgccgccctg aaaaagcgcc ttaataatca cgtaatacag
     5461 cacattggtg ctatgccgct ggataaatgc gagctacggc actggctggc ctgttttgac
     5521 caggtggcaa agcgaacgcc tgttactgcc ggattcttgc tacagacgtg caaacaggcg
     5581 cttaagttct gccggaggcg gcgctatgca atcagcaacg ttcttgatga tatgagtgtg
     5641 gcggacgttg ggaaaaaacc ggatataagc gagcgtgtct taagcaccaa agaactgggc
     5701 gaattattgc aggcactgga caaaaaaata ttctccccct actacatcgc gttaatccgc
     5761 ctcctgattg tgttcggatg ccggacggta gaactgaggt tatcggagat cagcgagtgg
     5821 gattttaccg aaatgctctg gaccgttccg aaggagcaca gcaaaacgaa ggtagccata
     5881 ttccggccta taccggaagc aatactgccg ttcatcacgc agctggtgga gcagaacagg
     5941 cacacgggct tattgctggg ggaagtgaaa caggaggcca gcgtatcgca gtatggaaga
     6001 ttagcgcaca ggaggcttaa tcaccctcac tggtcactgc atgacatccg gcgcaccttt
     6061 acaaccatgc tgaacgattt aggcgttgat ccgcatgtcg tggagcagct tacaggccac
     6121 cagatgccag gaatgcagcg agtttataat cattcccgtt atctggatgc gaaacgcaat
     6181 gcgctggata tgtggacgga gcggttaggg atactggcgg gaacacatga aaacgtaacc
     6241 acgctaccag tagccagaag aaaataattt ttttcgtgtt ttttcagtat gcgcatactg
     6301 gatatgcgaa cagatacaac gtgcaacaat gagcaacaat gtggaacaac tacgaacaag
     6361 aggcgaaaaa gcgtacggat ttataagtag ctgattttta atgtgttact ggttttttat
     6421 acactagcga atcactcttt aaagagcgag aaaaaaggca tggaacaata ttttatgcct
     6481 ttgtattaac atgaatttaa aagattttat tcttactttt tcattttctg taaatcatac
     6541 atcctcagta tttgccattc atcactctag cgggaacaat acgacacaat aagacacctg
     6601 atgactcttt aagaaacgaa agggggcaat agtgttaagc actgatcggt ttatacgtga
     6661 aaaagaatgc gaaaaactaa ccggccttag ccgtacgtgt cgctaccgcc tggaaaaggc
     6721 cggacaattc ccatcacgtc gtaaacttgg cggtcgttcc gttggctggt ctttatccga
     6781 ggttctggcc tggaaggata gctgcaaggc agttcattaa tcacgctggc ggcacacagc
     6841 cgccacacat caatcatctg aacacagagc tataaccatg aagattgaat atacgccaga
     6901 acgtgggcgg ggattcgttc gccctggtga gactggaaaa ccacaaaatt ggggtttttc
     6961 aggcataaaa aaagcggccc cgaaatggag ccgcctttct gaacagataa cccgctgcgc
     7021 cttatgtgta tatgatccca aacataagca cggggatgat agccgctatc aggctggtgg
     7081 gcaatgctat cagtctggtt cagttcgttg ccatacctgc aatgggcgcg tttccctgta
     7141 ctctttaagg aattgctcaa gggcaaaagc acatggcgcg aatctttctg attcatgctc
     7201 tatctttctg cgccgtcttt tccgtgccgg tgataatgtt ttggtcaatt ctttatcggt
     7261 cattgtgttg tcctgcatag caatgcgccg taatacctca caccacggcg ctgatagttt
     7321 ttatcctttg ggttctatgc cgcgttgctg taattctttg cggagaacgc gtttaatcca
     7381 agtggctaaa gatgtgtcgc catcggcctt agcagcttct tctagctgcg ctctgaagtc
     7441 ttccggtaat cgcatttggt atggaggcga acgtttttct aatggtgttg acatggtaat
     7501 taccctttat tgctatacct acatggtaat taccattgta cttgccatta cacaaaaatc
     7561 aacgccccgc agtgctggga acacatacgg ggcgtctaac caacaacgta aactaggagc
     7621 cgttatggtt gccgtaaatc atataccaca ccttgtacac acacaaacgg cctttgtgtg
     7681 gcgttttctg gcactgagtg ccggagaatc tcaaatcatc cacgtaaccg cctggacgga
     7741 acgcgaagcg cgtaaccgtt gcccgtccgg ttgtgttgct gtatttgccg cccgtattcg
     7801 tcaggaggtg cgccatgatt aatctgtcac ttactgacct taaccgcatt cagtttcgtg
     7861 agaaattcac agggcagcta ctggtcaatg tggataaagg tcgcgtggtg tgtaattacc
     7921 acttaccaga tgaagcaatt gtcgccacaa gggaatcatt acaggaaatc acaaaacgtg
     7981 ccggaatgat taacacgaat attcagggga tgctttatgc acaataaaac cacaccggac
     8041 gcagccgccg aagccatcaa aacgctgatg cacgcgctga ttgatatttc tgttatcgcg
     8101 gacagggcac ataagcacgc caccagcgaa acagaatatg ccggtgcttt cgttcctcac
     8161 tcactggctg tcatgcaatt tagtgctgat acggcgctga atgaggccaa agctatcctg
     8221 attgctgatt gtgaaaatgg gggggttatg cgtgatgatc gttttaattc cctgaaacag
     8281 gaattttccg gcgttcctga tgatgcggct gatgcgcttt cgtcaatgcc agaacttatt
     8341 agagcggctt ttttcttact ttccacgaga gaatataaat caacggggct tgatgtactg
     8401 aatatcgccg ccgattatgc ggaatatgtg gcagaggcgc gttatagaag aaaatttcct
     8461 gaggatgtaa gccatgcgtg atatttacca ccaaacaata gaccgcgcat ttcttgcact
     8521 ttctcacagt gaaaacatga tggaaatatt gcgcatatgg cttgaaacac ttggcgacaa
     8581 tgaacgcgac aaacaaaaat caagaattgc cacggcatta ataacgcttc ttgagcctgt
     8641 aataatggaa ctgcaagaaa tagatctatt gcacgacaga tataaagaac agcacaccgg
     8701 agaataaaaa taatgaaact taaatattct ggcttaactg ccagtggcaa cactcaccct
     8761 aaatttacgc gcggtgatat ttaccgcgac cagtacggcg gcacggtaat gattaagggc
     8821 gtggaagaac ggcgcgtaac ctaccgccgt gaaggttacg aatatgattg cgtgatgcct
     8881 gtttatcagt tccggcgtga tttttctctg gtacagaccg cgccgcataa cgtgcccacc
     8941 agcaacgcca gggcacgggc aaacatccag aagctgaaaa ccatggttaa cggattcagg
     9001 ggcaagaaat gaaaagcgca ccgaacttaa aaaaacagcc ttacgacaag atgaccgaag
     9061 tcattatttt tgcgggtagt gatgcctggg cacatgcgaa acagtggcag gaacaggacg
     9121 ggcgactggc tggcgataat gtgcctcccg ttgtgctggc tgatgatcaa ctggatgaac
     9181 tggcagaact gagaatcatc gacgaggggc gctattgtgt ccggctgtac aaggcaggcc
     9241 acatcaggcc atcaaatatt aatgccattg cgcacaagct ggcggcggcg ggtgtaactg
     9301 atgcgaatta ttaccccgaa gggatgcaca gccatatgcg ggagaactgg cgcgaatacc
     9361 tggaacgggt gcgcgggaaa gagccggcgg aagaaaaaaa ccaccagcga aaaaccacgc
     9421 taccgatgag cgttggatct accggatacg acacgcaact ggattacgtg gttaagggga
     9481 ttattccggc ggtatcgcta tgcagcatat acggggctag cgggtcctat aaatcattcc
     9541 ttgccggatc gtgggcgtgc catgttgcca ctggtcgcca gtggggaggc cgcagggttg
     9601 cacatggtgc ggttctctat gtggttggtg aaggcggtat aggtgttccg cgtcgtgtaa
     9661 aagcctggga ggttgtgcac gatgagcagg tgaaaaatct gtatctggta aaccgcccca
     9721 tctttccggc tgccccgctt gatgttgatg aaatggttat cgctgcccgt caggtggagc
     9781 gggaaacggg taaacctgta cgcatgatta ttctggatac gctggcgcgt tgctttggtg
     9841 ggaatgatga aaatgattcc cgtgatatgg gggcgtttat ccgtggttgt gacgaactga
     9901 aacgacgcac aggggccacg gtgctggtgg ttcaccattc cggcaaggat gagacgaaag
     9961 gcgcgcgcgg ttccagtgca tttcgtgctt cgctggatgc tgaataccgg atacgcaggg
    10021 aggacgcagg aagcgaagcg ctggttatct catgcaccaa aatgaaggac gcggaggaac
    10081 tcaaagaagc cgcatatgac ttacgcgtgg tggagctttt taccgacgct gacggtgaat
    10141 taatcacgtc gctggtggtg gtggatgatc cgcgccctcc tgttgaactg gagcgcatcg
    10201 aggaggcagg gaacaagacg gaaaaccata ccgcgctatg ggggtgcatc cgttcacgca
    10261 cacagaacgg cgacaagtgc acgatcccgc tgttacgtga tgacatgaaa aagctggggt
    10321 atgaaatgaa aaacttccgg cgctggctgt acaagctgga aaaagatggg gttattcgta
    10381 tcgatgggga tgatgtagcg ccgctataaa agtgaggagc aaaagcgagg gggatagaaa
    10441 gagggccaaa attagcccgc tctccctcac ttttcgacct gtatacatcc tcaaaagtga
    10501 ggggtaaaaa aatacttatg aaacacacac atagaaaaac cgaaaatccc aactgcgacg
    10561 aagtgagacg cttgaaaaag tgaggcgaaa aagtgagagg ttgcgagaaa tgacccaaaa
    10621 acgcagagac agaacagagc caaaatataa agcgttagac atgactgagc acaccttaaa
    10681 ggtggcaatc agaacgatag accgccacac gcgggaagga tacgcgaagg aacatcccga
    10741 cctgataagc gcattcatga ccacggcggc ggcaaacttt gccacgctga cagaacggga
    10801 gattgccgaa gcggaacagg taacaaccat caacgttaaa accggagagg tggaattatg
    10861 acggcacaga tagccgctta cgggcggctg gtggacgacc cgcaggtaaa acagaccagc
    10921 aagggcacac cgatgacgct ggcgcgtatg gcggtatctt tgccatgcag ccaggcacag
    10981 gacggacagg cgacgttatg gttatcggtc atcgcatttg gcaaacaggc cggcttcctg
    11041 gctaaacatc aaaaaggcga tgttgccagc gtatccggca cgatgcaggt aagccagtgg
    11101 accggacaga acggagaaac gcggcagggc tggcaggtta tcgcagacag cgtaatcagt
    11161 gcccgcgcgg cacgtccagg cgggaacaga cgcaaaacca caggcacaca gggtaatcag
    11221 ccaccagcgg gaggcgatga cccttacggt gatgatattt cgttctgagg gggtgacgat
    11281 ggtacatgac cgcatagcgg aggaactgga ggcgaaaggc ttttaccgga gggcggcggc
    11341 gcgatggggt gaagtcatgc agctggtgga gacagacaag gaacggcatc acatcacgat
    11401 gcgacggctg gaatgttcaa ggaaggcaca gagggcaccg gagccgccgg ataatttcgg
    11461 agacctgaaa aaggcagtcg atcgcactta tgccgaaatg ggtatagatg gtgctggtga
    11521 tgaaatatgg cgcaattacc aggacagcta atcaaacagc cggagaaatc cggctttttt
    11581 tgcaccagtt gaaacggtat ggcgcattac cgggttttcg tcacggtcag gcatagttac
    11641 tatctgaaac aaacagacac aacagaggaa aaaaacaatg ccgatgaaat ttgatgagat
    11701 attaaaacag cgtgataaat accatgctga caacatggag acgatgagca tcaatgatta
    11761 ccgcgcattc ctggagacgg gcgcactgat tgaaaaggat cagcatggtt ttgtgagatg
    11821 tgctctatcc ggtgaaatgc tggcggtaaa tcctgaacag atagatgcat tgatagaatt
    11881 tctgaaagag atcagagact gagccagcac acagcacaca tagccggagc aatccggctt
    11941 ttttgcgccc aaaaaaagcc cgataaggtc agagggttct tatcgggctt ttgcatatga
    12001 ggtttttgga tgcactggcg ttcgtgatcg ggataatcat ttcataattt gcaacataac
    12061 tcaatatcat tgcataaaat gcaattctga ttataatcag gactggataa acatccagtt
    12121 atgatttttt tagtcgaaga ggaatttctt actatggctg aagagaaaaa aagcggtgtt
    12181 tcggtgtaca taagccccga aatcgtggag gtgctcaagc agcgccacaa aaaaaactat
    12241 gaagctggcg ttgcggctgg actggatccg ctgatgacgc cggagccgtc gataggttca
    12301 cttgtacgct cttatttact tgcggcgctt gggatgcata aaaattatgg gggtgaataa
    12361 tggcaggcaa agcaacggca cttaacacta accagctttt catgtacctg aatcgcgggg
    12421 atattgcgga ttttaaattc agccctctgt ttaccacgct gtttttcccg aacgtggcga
    12481 cattcagcac gcaaaacatc atgctggata ccctggacat tgaagaagtc accatgtcgg
    12541 cgttttgttc gcctatggtg ggtagccagg ttcagcgcga taaagggtac gaaaccagca
    12601 caatcaaacc tggctacatg aagccaaagc acgaaatcga tccaacgaaa accatcatgc
    12661 gcatggctgg agaagatccg gcacagctta acgaccctac ctatcgccgt atgcgcctga
    12721 ttactggcaa catgcgccgc cagataaacg ccattaaggc acgcgtggaa tggctggcgg
    12781 tgaatgcggt aacgaccgga aaaaacatca ttgagggcga aggcatagaa cgctatgaaa
    12841 tcgactggaa aataccggaa aactgcatca tagagcaggc caagggtaaa aaatggtccg
    12901 agcaggataa agacatgcac gacccaatct atgacatcga gctttatgct gatcaggctg
    12961 gttgccccgc aaacgtcatg attatgggcg ctgaggtatg gcgcacatta cgcagcttta
    13021 aaaaattccg tgagctgtac gatctttccc gtggttcaga atccgccgcc gaactggcct
    13081 gtaaaaacct gggcgaagtg gtgagcttta aaggctatct tggtgatctg gcccttatcg
    13141 tctattccgg caaatacact gacagcgatg gtaccgaaaa atatttcctt gagcctgatt
    13201 tgctggtcct gggtaacacc aacaataaag ggctggtggc ctatggtgcg ataatggatc
    13261 aggaagcggt aagaacgggc gcaacacaaa acatgtttta cccgaaaaac tggattgagg
    13321 acggcgatcc ggcgattgag tacgtgcaga cacacagtgc accgcagccg gtaccggcag
    13381 atattcgcaa atttgttacc gtcaaaattg gttaacgggg ggattctatg gacactccat
    13441 acattgagtt atttgcaggc agtcagcagg tatccacgac gttggtacat tttgccgctg
    13501 atgctggcgt tattcaggaa tttaccccgc tgatgctggc agacaatggc gagtttaagc
    13561 cgtgggatgg tcaggaatct ggcaaggctg tttatctgac ctcgtaccct gtggacacgt
    13621 caaagcagaa atcagcacag tgttacaaga cggggatatt caatattgcc gccgttaact
    13681 ggcctgagag cgtcgacacc gatgcgaaaa aatgcgccgc ctttgcgggt tccggcgtat
    13741 ccgttcagcc gctggcccga taagcagggg gaacgatggc aacgaatgaa agcatcatga
    13801 cgctaccgct ggcgagtaaa tttaaagtcg aagcgcgggc aatggctgac agaggtttat
    13861 caacctatga ggccgtatat caactaaaca aactggaaga gcaggacaag ccgtgcgctg
    13921 atgcgattat ggcgcttcat gagcataaag actatcagcc gctgttgcgt gcaatggcaa
    13981 acgtgccttg tatcgatgtt ggtacggcta aaagcatcct tagcatgacc atagagcagg
    14041 aacgtccgaa ggttgcgccg gaacttaccg cagcctttga aaactttatg gacatgcaca
    14101 gcccgcaagc cgtatcagct ggcatggcat acgatggcag aaaccagggc gatgacggcg
    14161 acatcgatcg catactgaaa accatctgag acaaggccgg agaaatccgg cttttttacg
    14221 ggtccttcct ggaattatgg cccgttacgg ggcggcgacc tcgcggattt tcgctattta
    14281 tgagcctttt cagggtggtg gtggtggttt tgttgtttga tctatcttta tgaatgaaaa
    14341 gggaaagatg caagcaatac accaacctga agcagtaatt aagttggtgt attaatgaaa
    14401 tctcatctga tgaacaaaaa aaacatggcg aaaagctgcc gtgtaagtgc gacagcgttc
    14461 gacaagtggg gagtaactcc cgttgagcgt aaaggccgtg aggcgtttta tgatgttgcc
    14521 agcgtgatag aaaatagggt taacaatgca attagccagc ttataaacga caaaggcgag
    14581 attgacgatg atgaactttt acgagtcagg atcagattac tgacagcaca ggcagaggcg
    14641 caggaactta aaaacgatcg cgatcgcggt gacgtgattg atactgagtt ttgcctgtac
    14701 gcgctttcaa agctggcgag tcagatttca tcaatcatgg acagcctccc gcttactatg
    14761 caaaggagct tcccacagat tacccccgcc atgctggaca ggcttaaaag ggaagtggtt
    14821 aaagcctgta atgccagtgc cagagttgcc gacaacctcc cacagatact ggctgattac
    14881 ttgaaagaaa caaccggaaa cgtaccggaa aagttgctac agaagaaagg cgagtaacag
    14941 acgcgcaatt attgaacaaa aatgagaaag gatctgataa tggcattttc tggctaaaat
    15001 gcctcgtgtc agatcctttc tttttttaat attttcattg ttaaacagtc tgttacagag
    15061 aagaagatcg gatctgactt ttgccggaaa attctcataa acagataaaa atcgcgaggt
    15121 cgccgccccg taacgggcca taattccagg aaggacccga cgacaccaga ctatcagagc
    15181 gatgggggca caatgacaga agccgaacta ctgggattaa tccgccgcgt cgccggaatc
    15241 agccagcagc atgacgaaca ggccacacag ccggacagcg tgaccgctga aaattatgtg
    15301 cgtgttgtag cggaagtgat gcgccgtgat ggtatagaac ttaatggcgt tgatatgcgc
    15361 aacatacgga accgcgttct tgaaatgctg gcctacaatc gccgcgttga actgtatcgg
    15421 gagaaagaaa aaataacgta ccactggaag aagccggaac ggctgcggcg gtaacttgct
    15481 gatattcccg ataacgcaaa attgcgctgg ctacatcctc acatctgagg actgttaatc
    15541 agaaggccgg aatatattca caaactacga aattttcgta gtttaattaa tggtcagatt
    15601 aatcgctaac ccgctgatat tttcggaaac ctcaatttga ggaagtcgga agcgtcaccc
    15661 ttcatattct gaatggtcat aatgaccacg cagaccatca aggcaaccac aacgaacatc
    15721 gttacggttg gagcttttac cccactgggg gaaaggtatt acgataatca taacacctac
    15781 cgaacaggta aaacccacca gcctgattaa cagttaaccg gaaaaaaaat ccggtagccc
    15841 cgttatgggg atccccatat cgacattaac gcccctcatg aattggtgcg cttccccctg
    15901 gaaagattac ccgcctgtat atttcttgtg tctatttgtt ccacgttgtt tcatacagtg
    15961 caccgaacgg tgtagttaat ggtgtagtca atttgctgtt tttagcactt tttgcagtgt
    16021 gggttgttta attaaatcaa tgggttgcct tgctgtggtg ataaaaggac atatttattc
    16081 atctttcggc gtagaatgct ggcgaccaaa aatcacctcc atccgcgcac cgccaagcat
    16141 gctctctccg gcgacgattt taccctcata ttgcttggtg atttcgcggg ctaccgccag
    16201 ccccacacct tgcccagggc gtaaagtatc aacccgttga ccacggtcga aaatgacctc
    16261 tcgcttgctt aatggaatac cggggccatc atcctcgacc acaatataga gatgctcgtc
    16321 ggtttgcctt gcagaaattt cgacaaactc gaggcaatat ttacaggcat tatccagtac
    16381 attgcccatc acctcgacaa aatcgttctg ctcaccgaca aagctgatct ctggcgaaat
    16441 atcgagagag atattgaccc ctttgcgttg atacactttg ttcagcgccg aggtgagatt
    16501 gtccagcagt ggggcgaccg gatgcagctc gcggctgagc aatgtcccgc cgtgcatact
    16561 ggcacgatgc aggtagtagc caatttgctg tgaaatgcgg ctgatttgct ccagcattac
    16621 cggttcagca tcactgacgc tcatcttttc actacgtaga gaacgcagcg tactttgcag
    16681 caccgccagt ggcgttttca gactatgggt caggtcggtg agcgttgtac gatatttgtc
    16741 gtaacgttcg cgttcacttt ttaacaatcg gttcaggttt cgtaccagac tggtcagttc
    16801 tcgcgttgtg gctggattga gcaattcgcg gttatgttct tccagttcgc ggacttcttt
    16861 tgccagggct tcgatggggc gtaaactcca ccaggcggcg acccacagca gcgggatcac
    16921 taacagcaga ttggctgaga gcacatagat aaaccagctc cagaccatat aggaactttt
    16981 tagctccacc ggaatggtat ccaccaccac aatggttaac tttggcatcc gcgatgttgc
    17041 cgggtagacg tttaccgcca ccgagtgggt catctccgcg tcgtcatcat cttcccgcac
    17101 ttcctgcaac tgttgctgta tcgaatgatc tccactcagc aagaggctgg tatcgttaac
    17161 atccgcttca atttcatgaa aaccattcga tttcagccag tcaggctgga tcatcttcat
    17221 cagccagggc acgtcacgtt gcgcccataa aagctgcccg ttctcatcat aaattagcgt
    17281 catggtgggg ctttgcttgt cgatattttc gggtaactcg acatgcaact tattgttttc
    17341 ccacttcgca agggtataga acagattgct ctcgccacgt aacagccgaa acgtagtttt
    17401 atcgaaactg acgctataac cgatcagcgc gaccattccg taggcaagcg aaagcaccag
    17461 tactaccgct gccgttgcca acagaaaacg tacccgcagc gagagcggga aaaaaagacg
    17521 cagtaatttt ttcatcagcg caattcgaac aaatagccct ggccgcgaac ggtagtaatc
    17581 acttcttgcg gatattgtgc ctgaattttt ttgcgcagac gtcccatcag tacatcaatg
    17641 gtatggcttt cccgcagctc cgcatccgga tagagttgta gcattaacga atctttgctg
    17701 accactttgc cattattgcg tatcaacgtt tccataatgg tgtattcgaa cgcggtcagt
    17761 ttgatcactt cgtcattaat agataattca cggcgagaga gatcaacctg aaacgggggg
    17821 agcgaaatga cctgtgaagc caggccgcta ttacgccgca ttaatgcctg cattcgcgcc
    17881 atcacctctt caatatgaaa cggtttagtc acgtaatcat cagcaccggc acttaatact
    17941 tcgactttgt cctgccagct ttcacgggcg gttaatacca gaatcggcag tgaaacatca
    18001 ttgctacgcc agcggcaaat cagtgacaga ccgtcctcgt ctggcaatcc gagatcgaca
    18061 atcgcaatat ccggtaaatg ttcattgaga taataatcgg cttctttggc atcttctgca
    18121 tcatcgacct gatgaccagc atcctgaatc tgaactttaa ggtggtgacg taacaacgca
    18181 ttgtcttcaa caaccagtac gcgcattttt atttctccct gtcttaatta ttaaaatagt
    18241 gtaacgcgat tatgtggtta tgggggtaaa cattaaataa accagcgggg gaggggaggt
    18301 aaagtgaaaa aataaaaagc ggataatctt aataagcagg ccggacagca tcgccatccg
    18361 gcactggtac gagtttattt cagctcatca accatggtga tggcgcgacc aatgtagtta
    18421 gccggcgtca tcgctttcag gcgggctttc tcttcttctg gcaacgccag accgtcgata
    18481 aactgcttca tgccttcggc gtcaacgcgc ttaccgcgag tcagctcttt cagcttctcg
    18541 tacggttttt cgatgccata gcgacgcata actgtctgga ttggttctgc cagcacttcc
    18601 cagttgtgat ccagctcatc cagcagatgg tcacggttca cttccagttt gctcacgcct
    18661 ttcagggtgg attgatacgc aatcagcgca taaccgatac ccacgccgag gttacgcagc
    18721 acggtggagt cggtcaggtc acgctgccag cgggaaaccg gcagtttgct tgccagatgc
    18781 tgcaataccg cgttggaaag gcccagattc ccttcggagt tttcgaagtc gatcgggtta
    18841 actttatgcg gcatggtgga agaaccaatc tcaccagcaa tggttttctg tttgaagtgg
    18901 ttaagggcga tataacccca gacgtcacgg tcaaagtcga tcagaatggt gttgaagcgc
    18961 gcaacgcaat caaacagttc ggcaatgtag tcgtgcggtt caatctgggt agtgtacggg
    19021 ttccattgaa tacccagcga ggtgacgaac tcttcgctga actgatgcca gtcaacttcc
    19081 gggtaagcgg cgatgtgggc gttatagtta ccgaccgcgc cgttgatttt gccgaggatc
    19141 tccacctggt taagctggcg gtactggcgc tccatacggt aggcgacgtt tgccatctct
    19201 ttaccgatgg ttgacggcgt ggctggctga ccgtgggtac gggacagcag cgggatatcg
    19261 cgatactgaa cggcgagatc tttaatgcca tcaattagtt gacgccagta cggcaggatc
    19321 acttcatcac gcgcggtttt cagcattaat gcgtgggaga ggttattgat atcttccgaa
    19381 gtacaggcaa agtggatgaa ttcagaaacc gcgtgcagtt ccgggatctc cgccactttt
    19441 tctttcagga aatactcaac cgctttaacg tcgtggttag tagtacgctc gatggttttg
    19501 atgcgtgcgg catcttcttc gctgaaattg gcgacaattg catcaaggta accgattgcg
    19561 tcggcagcaa aagcaggaac ttccttgatc gctgcgtgcg cggccagttt ttgcagccaa
    19621 cgtacttcaa cttgtacacg gaatttcagc aaaccatatt cgctgaaaat cccgcgcagc
    19681 gcgctgactt tatcgccgta gcgtccatcg acaggggaaa cggcggtcag tgaggataat
    19741 tccatagatc acaactccgg ggttaaatga gcaagaattt gttttgcctg agtggtcagg
    19801 cgattacgag aaaacatcag ttgcagacgt ccgccgccga cctggtgcca gagcacggcg
    19861 gcgcgaatgc ctgccagcag ggttgcgcga actttcgcct gcacttgtgg gctttgcagt
    19921 acagcagggg aaccggtgac ctgaatgcgc gggccaagcg ggctgatcac atcaacatag
    19981 atagcagcca tcgcgctcat cagcgtttcg gactgtaaat cgaagtgttc gagctggcgt
    20041 tgcaggccgt tgatccggtt gcccagagtg tcgagcgcac ctttcgctga ggagagtttg
    20101 cgcttaagca ccatcaggct gagtgtgtag cgggttaatt cggcgtttaa gccctggcga
    20161 ctgctggcat tgagcacgcc gagcagggtt tccagcccga cgcgcaggtt ggcttcgcta
    20221 ccgccaaaaa ccgccagcgt cgagctgggg ttcatatcaa taatactgtt gagtgagacg
    20281 tgtagcgcat cggcatcaca atgcccctgg tgagcgagtt gttgcaccag gcgtgccgac
    20341 tgacaaatac cggccagggc gagggtgatg tcatagtaat tctttgccac gttcactgct
    20401 tccttgtttt aagtaaagat aataatcaga tcggcagcgg cagacgctgc tcaataatac
    20461 cgccaccgag gcacacttca ccgttataga agacggcaga ctggcccggc gtcacggcgg
    20521 caacgggctc atcgaaaatc acttcaatgc gatcatcgtc cagcgccttg acggtgcagg
    20581 ggatatcggt ctggcgatag cgggttttta ccgtgcaacg catagtaccg gtgaatggtt
    20641 cacgatcgac ccagtgcagc tgctgggcaa tcagaccgac agacatcaga cgcgggtgtt
    20701 catggccctg cgcaacaatc agaatgttgt tttcgacgtc tttatcgacc acataccacg
    20761 gttcttcggt gccttctttg gtgccaccga tacccagacc tttgcgctga ccgagggtgt
    20821 gatacatcag cccctgatgc tcaccaattt catcgccatc gactgtaatg attttgcccg
    20881 gttgcgccgg gagataacgg ccaaggaact cgcggaattt acgttcgcca atgaagcaaa
    20941 tgccggtaga gtcttttttc ttcgcggtga ccagaccaag atcttcagca atcttacgca
    21001 cctgcggttt ttccagttcg ccgaccggga acaggctttg cgcaatctgc tcatggctga
    21061 gcgtataaag gaagtagctc tggtctttat tgctgtccag accacgcagc aggcggctct
    21121 tgccatcgac atctgcccga cgaacgtaat gaccggtagc gatataatcg gcacctaaat
    21181 cttcggcggc aaattcgagg aaggctttaa atttgatctc tttgttgcac agaatatccg
    21241 gattcggcgt gcgaccggct ttatattcgg caaggaacag ttcgaagacg ttgtcccagt
    21301 actcggcagc aaagttaacg gtgtgcagtt caatgccgag cttgtcgcag acagcctggg
    21361 catcagccag atccgctgcc gctgtgcaat attcctcacc gtcgtcttct tcccagttct
    21421 tcataaacag gccttcgacc tgatatccct gttgttgcaa cagccaggca gaaacggagg
    21481 aatcgacacc gccggacatg ccgacgatta ctttttttgc ggtttcagac attggatcac
    21541 tcacgacatt gaacttcaag gcggcgtatt ctatcacgca gccttttaga tgacaccctt
    21601 tgtaaaaggc cagttaaaat cgccaatcat ctccagcgga tagcgttgcc cgctttgata
    21661 acaacggata ctttccgcca ccagcggcga acgaagattt gacgcctgta aaatttcttc
    21721 ggcgctgacc caacgacagc agtcgatatc gctgtcatga ggctgggtcg ggcatatttg
    21781 ctcaagctca atggcaaaga ggaaacgcaa aaacggcgtt ttatctggcg caatccactg
    21841 atgcatacga ataaagtgtt gcggctgcgc gctgatgccg gtttcttccc acagctcacg
    21901 ggcggctgct tccactaagg tttcatcggc ttccagatgc ccggcaggtt ggttccataa
    21961 ggctttacca ttaatcgtct cttcaacgac taaaaatttc ccctctgcgt gcactacgca
    22021 agcaacggta acgtgcggtt taaacattgt gcatccttat tctgttgctt ctcgccattc
    22081 gccattggca aggttatcca aagagtaatc acccatcgca tagcgaatca gtcgcagcgt
    22141 ggggaaacca acatgggcgg tcatgcggcg cacctggcga ttacgtcctt catataaggt
    22201 gatcttcagc cagctggtgg gaatactttt gcgttcacga attggtggat tccgcggcca
    22261 taaccacgcg ggttcgtcaa ccagctccgc gcctgcgggc agggtagggc catcatttaa
    22321 ggttacgcca ttgcgcaagg cttcaagtgc gtcttgtgtg ggaatacctt ccacctgcac
    22381 ataatagatt tttccggtgc gtttgcccgg ctgggttaaa cgcgcctgca acgcgccgtt
    22441 attggtcagc accagtaacc cttcgctatc gcggtcaaga cgacctgctg cataaacgcc
    22501 ctgaaccggg atgaattctt ttaatgtttt gcgtccggct tcatcggtga actgcggaag
    22561 aacatcgtag ggtttattga acaggatcac acgcgtgggc tggttttcag gtttacgcct
    22621 ggtagaacgt tgtgagctga atcgcttaac ctggtgattt ctaaaagaag ttttttgcat
    22681 ggtattttca gagattatga attgccgcat tatagcctaa taacgcgcat ctttcatgac
    22741 ggcaaacaat agggtagtat tgacaagcca attacaaatc attaacaaaa aattgctcta
    22801 aagcatccgt atcgcaggac gcaaacgcat atgcaacgtg gtggcagacg agcaaaccag
    22861 tagcgctcga aggagaggtg aatggaaagt aaagtagttg ttccggcaca aggcaagaag
    22921 atcaccctgc aaaacggcaa actcaacgtt cctgaaaatc cgattatccc ttacattgaa
    22981 ggtgatggaa tcggtgtaga tgtaacccca gccatgctga aagtggtcga cgctgcagtc
    23041 gagaaagcct ataaaggcga gcgtaaaatc tcctggatgg aaatttacac cggtgaaaaa
    23101 tccacacagg tttatggtca ggacgtctgg ctgcctgctg aaacccttga tctgattcgt
    23161 gaatatcgcg ttgccattaa aggcccgctg accactccgg ttggtggcgg tattcgctct
    23221 ctgaacgttg ccctgcgcca ggaactggat ctctacatct gcctgcgtcc ggtacgttac
    23281 tatcagggca ctccaagccc ggttaaacac cctgaactga ccgatatggt tatcttccgt
    23341 gaaaactcgg aagacattta tgcgggtatc gaatggaaag ctgactctgc cgacgccgag
    23401 aaagtgatta aattcctgcg tgaagagatg ggcgtgaaga aaattcgctt cccggaacat
    23461 tgcggtatcg gtattaagcc gtgttctgaa gaaggcacca aacgtctggt tcgtgcagcg
    23521 atcgaatacg caattgctaa cgatcgtgac tctgtgactc tggtgcacaa aggcaacatc
    23581 atgaagttca ccgaaggcgc gtttaaagac tggggctacg agctggcgcg tgaagagttt
    23641 ggcggtgaac tgatcgacgg cggcccgtgg ctgaaagtta aaaacccgaa caccggcaaa
    23701 gagatcgtca ttaaagacgt gattgctgat gcattcctgc aacagatcct gctgcgtccg
    23761 gctgaatatg atgttatcgc ctgtatgaac ctgaacggtg actacatttc tgacgccctg
    23821 gcagcgcagg ttggcggtat cggtatcgcc cctggcgcaa acatcggtga cgaatgcgcc
    23881 ctgtttgaag ccacccacgg tactgcgccg aaatatgccg gtcaggacaa agttaaccca
    23941 ggttctatta ttctctctgc tgagatgatg ttacgccata tgggttggac tgaagcggct
    24001 gacctgattg ttaaaggtat ggaaggcgca atcaatgcca agaccgtaac ctatgacttc
    24061 gaacgtctga tggaaggcgc taaactgctg aaatgttcag agtttggtga cgcgatcatc
    24121 aagaatatgt aatcactaca tgtgttaaat attgaaacgg gcgtataaca cgcccgttgt
    24181 tttatgatga tttaaaatct tccccaaaac tttccccaaa acccttcccc aaaactggct
    24241 attttctatg ctgttttgat atctacgata atccagtctt taccacgatc atcattgtat
    24301 cggtcggtca tttttctgga tttatgacct aacaactttt gcgtatccag accctgttcc
    24361 cgatataacc gttctgacag agatcgctgc tcatgaaatg tgggcgcagt tccttgctcc
    24421 cattttatgc cacatttttc cctggccttt ttaaaagccg ttgtaagagt atttgcagac
    24481 acctggtctc ctctgtttgc ttgagaggta gtgtgacggt aatggaccag atatttacta
    24541 acaacagcat ccctgcactg agatataact tcacgaaggg taatattcag agcatcgcat
    24601 ttcaggttaa gcggaatagc aagttttgaa ccggtttttt cctgagtaat gtgcaacatg
    24661 tcgtcccaga tatcagagaa tttcaaattg cagatatcgc ttaaacgttg tccagtgaca
    24721 agagcaagta gcatcccgca ttttaaatag ggctgccgtc tgcttacgct gtcaaatatt
    24781 gcctgccatt cgggcagtga taatctttgg cggtttactc gatttcgcgg ttgttttgtt
    24841 gcctgcgctg ggttaaatcc tggcggaaca tgtcctgcgt gttgtgcttc tttgaagacg
    24901 tcgatcaaca ccattctcac gacttgcgcc atcctgttat gaccttcagc ctttacagca
    24961 tcaattattt cggcaatatc aagtgcggta atatccttga ggtgttgcat tccacaatgc
    25021 tcacggaaaa gacggatggg tttgcctttt tgccgatagg agttgggtct tagttcatta
    25081 tgttgcagcc tgtcctcctg gatagaatta tatttatcaa gccattctgt caccgtaatg
    25141 tctgagcgcc tgcctttcat tctttccaga cgctcattga cgcttaatat ttgtcgggta
    25201 cgttgttcag caataatggt atttgcttca gtagcaactt gttttgcttc attctcatca
    25261 gttcctaagc tatgaaaacg accggatagt ggatgtttgt attgccaata tacctttccg
    25321 gttcgcttat ctaatttgca atataaattg ggtatagaga ttttgtgaga tcgggggcta
    25381 gcagccatca gcgattatcc gttggagttt tgggtttgcg tttattggga gttgcggttc
    25441 tgcaagcgtt cctacaaaac gggaatttcg gtcaatcatc cagtagcgac caacttttat
    25501 agcgggtggg gccatcattt tcccttgcgc gtattttttc agaactcgct cacttggtgc
    25561 taagtcccca aattcttctt tagcccagtc ccgtaaagtg attagtcgag acatttgtcc
    25621 tcctcttagc tgctgaggga gtttgtgacc gatatatctg acatgatatt aagctcatgg
    25681 caggtacatc tcttgactgg tcatagagat aaatttaatg ctgagaaatg cagtattgaa
    25741 tttatcaatt tttcaccagc ttctggtctt tctggctgtc atgcgcaaaa catatggctt
    25801 taataaaaaa ctggattggg ttagcaacga gcaactttcc gaattaaccg ggatattgcc
    25861 gcacaagtgt tctgctgcaa aaagtgttct ggtaaagcgt gggattttta ttcagagcgg
    25921 gcggaatacc ggcattaata atgtggtcag tgaatggtca acattacccg aatcaggtaa
    25981 gaaaaataaa gtttacctga aagaggtaaa tttacctgaa tcaggtaaaa aaagtttacc
    26041 caaatcaggt aaaggcgttt acccgaatca ggtaaacaca aaagacaaac taacaaaaga
    26101 caatataaaa cctttttcgt ccgagaattc tggcgaatcc tctgaccaac cagaaaacga
    26161 tcttcctgtg gtgaaaccgg atgctgc
//
LOCUS       DAZKIP010000056        26176 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZKIP010000056 DAZKIP010000000
VERSION     DAZKIP010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26176
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.056"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..636
                     /locus_tag="ACHOUK_002581"
     CDS             <1..636
                     /locus_tag="ACHOUK_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFP9898000.1"
                     /translation="IITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFP
                     LKELNNPEHDSYAMSEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSI
                     IAEQKKESEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVVNEDDCKIRRG
                     NAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVFAGSGLS"
     gene            902..3139
                     /gene="nrfB"
                     /locus_tag="ACHOUK_002582"
     CDS             902..3139
                     /gene="nrfB"
                     /locus_tag="ACHOUK_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFP9898001.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            3126..6098
                     /gene="nfrA"
                     /locus_tag="ACHOUK_002583"
     CDS             3126..6098
                     /gene="nfrA"
                     /locus_tag="ACHOUK_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFP9898002.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSITTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAEALRLARLLYQQESANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARISQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAARQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRFFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            6099..6989
                     /locus_tag="ACHOUK_002584"
     CDS             6099..6989
                     /locus_tag="ACHOUK_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFP9898003.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAVPGVTPDGWYFSAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEHVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            7172..7933
                     /gene="envY"
                     /locus_tag="ACHOUK_002585"
     CDS             7172..7933
                     /gene="envY"
                     /locus_tag="ACHOUK_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFP9898004.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(7979..8055)
                     /locus_tag="ACHOUK_002586"
     tRNA            complement(7979..8055)
                     /locus_tag="ACHOUK_002586"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8019..8021),aa:Arg,seq:tct)
     gene            8298..8930
                     /gene="fimZ"
                     /locus_tag="ACHOUK_002587"
     CDS             8298..8930
                     /gene="fimZ"
                     /locus_tag="ACHOUK_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFP9898005.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
     gene            complement(8933..9448)
                     /gene="sfmF"
                     /locus_tag="ACHOUK_002588"
     CDS             complement(8933..9448)
                     /gene="sfmF"
                     /locus_tag="ACHOUK_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFP9898006.1"
                     /translation="MRRVFFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALLPFSLRLEGCPPGSVAILFTGTPASDANLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIFMINYN"
     gene            complement(9459..10466)
                     /gene="fimH"
                     /locus_tag="ACHOUK_002589"
     CDS             complement(9459..10466)
                     /gene="fimH"
                     /locus_tag="ACHOUK_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFP9898007.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            complement(10479..13088)
                     /locus_tag="ACHOUK_002590"
     CDS             complement(10479..13088)
                     /locus_tag="ACHOUK_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFP9898008.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTFVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGVRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQAAYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGGKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            complement(13119..13811)
                     /gene="fimC"
                     /locus_tag="ACHOUK_002591"
     CDS             complement(13119..13811)
                     /gene="fimC"
                     /locus_tag="ACHOUK_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFP9898009.1"
                     /translation="MMTKIKLLMLIIFYLIISASVHAAGGIALGATRIIYPADAKQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(14031..14573)
                     /gene="fimA"
                     /locus_tag="ACHOUK_002592"
     CDS             complement(14031..14573)
                     /gene="fimA"
                     /locus_tag="ACHOUK_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFP9898010.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            15053..15919
                     /gene="folD"
                     /locus_tag="ACHOUK_002593"
     CDS             15053..15919
                     /gene="folD"
                     /locus_tag="ACHOUK_002593"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFP9898011.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            15921..16133
                     /gene="ybcJ"
                     /locus_tag="ACHOUK_002594"
     CDS             15921..16133
                     /gene="ybcJ"
                     /locus_tag="ACHOUK_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFP9898012.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            16241..16762
                     /locus_tag="ACHOUK_002595"
     CDS             16241..16762
                     /locus_tag="ACHOUK_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP9898013.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FNFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(16798..18183)
                     /gene="cysS"
                     /locus_tag="ACHOUK_002596"
     CDS             complement(16798..18183)
                     /gene="cysS"
                     /locus_tag="ACHOUK_002596"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFP9898014.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKVEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            18357..18851
                     /gene="ppiB"
                     /locus_tag="ACHOUK_002597"
     CDS             18357..18851
                     /gene="ppiB"
                     /locus_tag="ACHOUK_002597"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFP9898015.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            18854..19576
                     /gene="lpxH"
                     /locus_tag="ACHOUK_002598"
     CDS             18854..19576
                     /gene="lpxH"
                     /locus_tag="ACHOUK_002598"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFP9898016.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRTKVHKPWLQTLFLALPLFVRKRIAVRMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            19694..20203
                     /gene="purE"
                     /locus_tag="ACHOUK_002599"
     CDS             19694..20203
                     /gene="purE"
                     /locus_tag="ACHOUK_002599"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFP9898017.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            20200..21267
                     /gene="purK"
                     /locus_tag="ACHOUK_002600"
     CDS             20200..21267
                     /gene="purK"
                     /locus_tag="ACHOUK_002600"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFP9898018.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAE
                     RSEWPAVFERLGELAIVKRRTGGYDGRGQWRLRADETEQLPAECYGECIVEQGINFSG
                     EVSLVGARCFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLSATLEA
                     LIPLLPPEYASGVMWAQSKFC"
     gene            complement(21406..22299)
                     /locus_tag="ACHOUK_002601"
     CDS             complement(21406..22299)
                     /locus_tag="ACHOUK_002601"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFP9898019.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVRTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTDDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGMPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(22296..23111)
                     /locus_tag="ACHOUK_002602"
     CDS             complement(22296..23111)
                     /locus_tag="ACHOUK_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFP9898020.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(23122..24381)
                     /locus_tag="ACHOUK_002603"
     CDS             complement(23122..24381)
                     /locus_tag="ACHOUK_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFP9898021.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGASGMEAAR
                     AVSEEMAEIYLERNMQLQIPSWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(24391..26058)
                     /locus_tag="ACHOUK_002604"
     CDS             complement(24391..26058)
                     /locus_tag="ACHOUK_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFP9898022.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSACQKLPDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIMPASSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDAHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPTLRNQLITDLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSPAAQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
BASE COUNT         6496 a         6889 c         6512 g         6279 t
ORIGIN      
        1 atcatcacaa ctgatgcgat gggttgccag aaagatattg cagagaagat acaaaaacag
       61 ggaggtgatt atttattcgc ggtaaaagga aaccaggggc ggctaaataa agcctttgag
      121 gaaaaatttc cgctgaaaga attaaataat ccagagcatg acagttacgc aatgagtgaa
      181 aagagtcacg gcagagaaga aatccgtctt catattgttt gcgatgtccc tgatgaactt
      241 attgatttca cgtttgaatg gaaagggctg aagaaattat gcgtggcagt ctcctttcgg
      301 tccataatag cagaacaaaa gaaagagtca gaaatgacgg tcagatatta tatcagttct
      361 gctgatttaa ccgcagagaa gttcgccaca gcaatccgaa accactggca cgtggagaat
      421 aagctgcact ggcgtctgga cgtggtagtg aatgaagacg actgcaaaat aagaagagga
      481 aatgcagcag aattattttc agggatacgg cacattgcta ttaatatttt gacgaatgat
      541 aaggtattca aggcagggtt aagacgtaag atgcgaaaag cagccatgga cagaaactat
      601 ctggcgtcag tctttgcggg gagcgggctt tcgtaatctt gccctgcctg ctcacctcca
      661 ggcacttcca ctacatcatg acctgatcgg tgccaccact cctcttcact atgacgttaa
      721 cctcccctcc ttgacaccac gcttttgtct acattctacg caaacggcaa cttttataac
      781 cagtgtaaaa ataacgtgcc gcaatagtca tacctattaa tggtaaaaag ctgtcacaat
      841 tcataaaaaa ccttaatata cgccacccta aacataacca gcgttaatgt aaggtttttg
      901 tgtggactgg cttcttgatg tttttgctac ctggctctat ggcttaaaag taatcgcgat
      961 aacgttagcg gtcatcatgt tcatcagcgg gctggacgat ttttttattg atgtcgtcta
     1021 ctgggtacgc cgcattaaac gcaagttgag tgtttatcgc cgctacccgc gaatgagtta
     1081 ccgcgaactg tataaaccag atgaaaaacc gttagcgatt atggttccgg cgtggaatga
     1141 aacgggcgtc atcggcaata tggccgagct ggcggcgacc acgctcgact acgaaaacta
     1201 tcatatcttt gttggcacct accccaacga cccggacact cagcgtgatg ttgacgaagt
     1261 gtgcgctcgc ttcccgaatg tgcataaggt agtctgcgcg cgtcctggcc ccaccagcaa
     1321 agccgactgt ctgaacaacg tgctggacgc catcacccag tttgagcgta gcgccaattt
     1381 cgcttttgct ggttttattc tgcatgacgc cgaagatgtg atttcaccga tggaattgcg
     1441 tctgttcaac tatctggtcg agcgtaaaga tctgattcag atcccggtgt atccgtttga
     1501 acgcgaatgg acgcacttca ccagcatgac ttacattgat gagttttcag agctgcatgg
     1561 caaagatgtt ccggtgcgtg aagccctcgc cggacaagtg cccagcgcag gcgtcggcac
     1621 ctgtttcagc cgccgcgccg tgaccgctct gttagctgac ggtgacggta ttgctttcga
     1681 cgtgcagagt ctgactgaag attacgacat tggcttccgc ctgaaagaaa aaggtatgac
     1741 ggaaattttt gtccgttttc cggtggtgga cgaagccaaa gaacgcgagc agcgtaaatt
     1801 tttacagcac gcacggacgt caaacatgat ctgcgtgcgc gaatatttcc ccgatacctt
     1861 ttcgactgcg gttcgacaaa aatctcgctg gatcatcggc attgttttcc agggctttaa
     1921 aacccataaa tggacctcca gcctgacgct gaactacttt ctctggcgcg accgcaaagg
     1981 ggcaatcagt aactttgtca gcttcctcgc aatgctggtg atgatccagc ttttgctgtt
     2041 gctggcgtat gaaagtttgt ggcccgatgc ctggcatttc ctttctattt ttagcggcag
     2101 cgcatggtta atgaccctgc tgtggctaaa ctttggcttg atggttaacc gcatcgtgca
     2161 gcgggtgatt ttcgtcactg gctactacgg cctgacgcag gggctactat ctgtcctgcg
     2221 tcttttctgg ggcaacctga ttaactttat ggccaactgg cgcgcgttaa aacaggtact
     2281 tcaacacggc gatccacgtc gcgtcgcgtg ggataaaaca acgcatgact tccccagcgt
     2341 cactggcgat acccgctcgt tgcgcccgtt aggtcaaatt ctgctggaaa atcaggtcat
     2401 cactgaagaa cagctcgata cagcactgcg taatcgcgtc gaaggtctac gcctgggcgg
     2461 ttcaatgctg atgcaggggc tgattagcgc cgagcagctg gcacaggcgc tggcagagca
     2521 aaacggcgtg gcgtgggaat ccatcgatgc ctggcagatc ccgtcctcgc tgattgccga
     2581 aatgccggcc tccgtggcgc tgcattatgc ggtactgccg ctgcgtctgg aaaatgatga
     2641 gttaattgtc ggcagtgaag atggtattga cccggtttcg ctggcggccc tgacgcgtaa
     2701 agtcggacgc aaagtgcgtt atgtcattgt tctgcgggga caaattgtca cggggttacg
     2761 tcactggtat gcacgccgac gcggtcacga tccgcgggca atgttgtaca atgcggttca
     2821 gcatcagtgg ctcacggaac agcaggccgg tgaaatctgg cggcaatatg tgccgcatca
     2881 gttcctgttc gccgaaatac tgaccacgct cggtcatatt aatcgttcag caattaacgt
     2941 gttgttattg cgccatgaac gcagttctct gccgctcggc aagtttttgg tcaccgaagg
     3001 cgttatcagc caggaaacgt tggatcgcgt cctgacaatt caacgcgaat tacaagtttc
     3061 gatgcaatca ctattactca aagcaggttt aaacacagaa caggttgcgc aactggagtc
     3121 cgaaaatgaa ggagaataac cttaatcgcg tcatcggatg gtctggttta ctgctgacgt
     3181 ctttattgag taccagcgca ctcgcagaca atatcggcac cagcgcagaa gagctggggc
     3241 tgagcgatta tcgccatttt gttatttatc cccgtctcga taaggcgctg aaggcacaga
     3301 aaaataacga cgaagcaacc gccatccgcg aatttgaata tatacaccag caggtgccgg
     3361 ataatattcc gctgacttta taccttgcgg aagcctatcg ccattttggt catgatgacc
     3421 gggcacggct gttgcttgag gatcaactga aacgtcaccc aggagatgcc cgacttgagc
     3481 gcagtctggc ggctattccg gttgaagtga aaagcattac aactgttgaa gaactgcttg
     3541 cccagcaaaa agcgtgcgat gctgcgccga ccctgcgttg tcgcagtgaa gtcgggcaga
     3601 atgccctgcg gctggcacag ttacctgtcg ccagagcgca actgaacgac gcgacgtttg
     3661 ctgcatcgcc ggaaggaaaa acgctgcgaa ccgatctgct gcaacgggca atctacctga
     3721 aacaatggtc ccaggcagat acgctataca atgaagcacg ccagcagaac acattaagcg
     3781 cggcagaacg ccgtcagtgg tttgacgtgc ttcttgccgg gcagctggac gatcggatcc
     3841 tggcactgca atcacagggg atcttcaccg atcctcagtc atatattact tacgcgaccg
     3901 cgctggctta tcgtggcgaa aaagcacgcc tccagcatta tctcattgaa aataagccgc
     3961 tgtttaccac ggacgcacaa gagaaaagtt ggctctatct gttatctaaa tacagcgcca
     4021 accccgttca ggcattggcg aattatacgg tgcagtttgc cgataaccgc cagtatgttg
     4081 ttggcgcgac gctaccggtg ctgttaaaag aaggtcagta cgacgcagcg caaaaactgc
     4141 tcgccaccct ccccgccaat gaaatgcttg aggagcgtta tgctgtcagc gtggcgaccc
     4201 gtaacaaggc tgaagctctg cgtctggcac gcttgctgta tcagcaagaa tcggcaaatc
     4261 ttacccgcct ggatcaacta acctggcaac tgatgcagaa cgagcagtca cgggaagctg
     4321 ccgatttatt gctgcaacgc tatcctttcc agggcgatgc gcgtatcagc cagactttaa
     4381 tggcgcgact ggcgtctctg ctggaaagtc atccttacct ggcaacgccg gcgaaggtgg
     4441 cgattttatc gaaaccctta ccgctggcgg agcaacgtca gtggcaaagt cagttgccgg
     4501 gtattgcaga taattgcccg gcaatagttc gcttgctggg cgatatgtcg ccttcctacg
     4561 atgccgccgc ctggaaccgt ctggcaaagt gttatcggga cacgctaccc ggtgtggcgt
     4621 tgtatgcatg gcttcaggcc gaacaacgac aaccgaacgc ctggcaacat cgtgcggtag
     4681 cctatcaggc gtatcaggtt gaggactacg ccaccgcact ggcggccagg cagaaaatca
     4741 gtcttcacga catgagcaat gaggatctgc ttgctgctgc caataccgcc caggcggcag
     4801 gaaatggtgc ggctcgcgat cgctggctac aacaggcaga acaacgtgga ctgggaagca
     4861 atgccctcta ctggtggctg catgcgcaac gttacattcc tggtcagccg gaactcgcac
     4921 tgaacgatct cacgcgctca atcaatattg cgccttctgc caacgcttac gttgcgcggg
     4981 cgacaattta tcgccaacgt cataatgtcc cggccgcggt gagtgatttg cgcgccgcgc
     5041 tggaactgga accgaataat agcaacaccc aggcagcgct tggttacgcc ttgtgggata
     5101 gcggtgatat cgcacagtcg cgggaaatgc tcgaacaggc gcataaaggg ctaccggacg
     5161 atccggcact gatccgacaa ctggcctatg tgaaccagcg tctggatgac atgcctgcga
     5221 cgcagcacta cgcccggctg gtgattgatg acattgataa tcaggcgctg ataaccccac
     5281 tgaccccaga gcaaaatcag caacgcttca atttccgccg tctgcatgag gaggtcggtc
     5341 gccgctggac gttcagtttc gattcttcca tcggcttgcg ttccggggca atgagtaccg
     5401 ctaacaataa tgttggcggc gcagcgccag ggaaaagcta tcgtagctac gggcaactgg
     5461 aagccgaata ccgcatcgga cgcaatatgc tgctggaagg cgacctgctc tcagtttata
     5521 gccgcttctt tgccgatacc ggagaaaacg gggtgatgat gccggtgaaa aatccgatgt
     5581 ccggcaccgg tctgcgctgg aagccgctgc gcgatcagat ctttttcctc gccgtcgaac
     5641 agcagttgcc gctgaacggg caaaatggcg catccgatac catgctgcgc gccagcgcct
     5701 cattctttaa tggcggcaaa tacagcgacg aatggcaccc gaacggttca ggctggtttg
     5761 cccaaaacct gtacctcgat gcggcgcaat atatccgcca ggatattcag gcgtggacgg
     5821 cagattatcg cgtcagctgg catcagaagg tagctaacgg acagactatt gagccttacg
     5881 ctcacgttca ggacaacggc tatcgtgata aaggcactca gggcgcgcag cttggcggtg
     5941 tcggggtccg ctggaatatc tggaccggcg agacgcacta cgacgcctgg ccgcacaaag
     6001 tcagtctcgg cgtcgagtat caacatacct ttaaggcgat taatcaacgt aacggagagc
     6061 gcaacaacgc gtttctcacc attggagtgc actggtaaat gcgtaagttc attttcgtat
     6121 tgctgacact gcttttggtc agcccttttt cctttgcgat gaaaggtatt atctggcaac
     6181 cacaaaaccg tgatagtcag gttaccgata cccagtggca ggggctgatg agtcagttac
     6241 gtttgcaagg cttcgatacc ctggttttgc aatggacccg ttacggcgat gcattcaccc
     6301 agccagaaca gcgcgcgtta ttgtttaagc gggccgcagc tgcgcaacag gctggcctga
     6361 agctgattgt cgggctgaac gccgatccgg aattttttat gcaccagaaa cagtcatccg
     6421 cggcgctgga aagctatctt aatcgcctgc tggctgccga tctccagcaa gccagattat
     6481 ggagtgccgt acctggcgta acgccggatg gctggtactt cagcgctgaa attgacgacc
     6541 tgaactggcg cagcgaagcc gcccgtcagc ctttgctaac atggttaaac aacgcgcagc
     6601 ggctgattag cgatgtttca gcaaaaccgg tttatatcag tagttttttc gccggaaaca
     6661 tgtcgccaga tggctatcgc caactgctgg aacacgttaa agcaaccggc gttaatgtct
     6721 gggtacagga tggcagcggc gtggataaac ttaccgctga acagcgtgaa cgttatctac
     6781 aggccagcgc cgattgccaa agctccgccc cagcaagcgg cattgtttat gaactgtttg
     6841 tcgccggcaa aggcaaaacc tttacagcga aaccgaaacc ggacgcagaa attgcctcgc
     6901 tgttagcgaa acgttcctct tgcggtaaag acactctcta tttctctctg cgctatttgc
     6961 ccgtcgcgca cggcattctc gagtattaaa tctcctccag gtaagtcggg tacgacctgg
     7021 cttacctctt tcgctctcgt cagaattcca tcaagattaa ttgctaaaaa gcgtgctaaa
     7081 gaaagaagat cgttatcggc gaacgggccg atcactcatg caggaagcat agatcctttg
     7141 agcggagttt ctgatatctg aggagtgcga aatgcaattg agcagcagtg aaccttgcgt
     7201 ggtgatcctg accgaaaaag aggtagaggt aagcgtcaat aaccatgcta cgtttaccct
     7261 tccgaaaaac tacctggccg ccttcgcgtg caacaataac gtcattgaac tctcaacgtt
     7321 aaatcacgta ttaatcaccc acatcaaccg taacatcatc aacgattatc tgttgttttt
     7381 aaataagaac ttaacctgtg taaagccctg gtcgcggctg gctaccccgg ttatcgcttg
     7441 tcatagccgt acaccggaag tgttccggct agccgccaac cacagcaagc agcaacccag
     7501 caaaccctgc gaagcggagt tgacgcgcgc attgcttttt accgtattgt ctaactttct
     7561 tgagcaatcg cggtttattg ccctactgat gtatatctta cgcagcagcg tccgcgacag
     7621 cgtctgccgc attattcaaa gcgatattca gcattactgg aatctgcgaa ttgtcgccag
     7681 ttcgctatgt ttaagcccca gcctgctcaa aaagaaatta aaaaacgaaa ataccagcta
     7741 tagccagatt gtcacagagt gtcgtatgcg ttacgccgta cagatgttat tgatggataa
     7801 caaaaatatc actcaggtgg cgcaattatg tggctatagc agcacgtcgt actttatctc
     7861 tgtttttaag gcgttttacg gcctgacacc gttgaattat ctcgccaaac agcgacaaaa
     7921 agtgatgtgg tgaagggcaa agcggaaacg gataagacgg gcataaataa agaagaaatg
     7981 gcgcgccctg caggattcga acctgcggcc cacgacttag aaggtcgttg ctctatccaa
     8041 ctgagctaag ggcgcgttga taccgcaatg cggtgtaatc gcgtgaatta tacggtcaac
     8101 ccttgctgag tcaatggctt ttgatctggt tgctgaacaa gtgaacgacc gcgtctgatt
     8161 ttctgattta tttcgctata gcggcaaaca aacgcacacc gctgcgcgtc tgaatcaaga
     8221 aaacccgtat tttcatgtat caaagtacaa tttaccgccc taacggaaaa ttgtccgctc
     8281 ctatgagact ggtaactatg aaaccaacgt cggtgatcat tatggatact catcctatca
     8341 tcagaatgtc tattgaagtt ctgttgcaaa aaaacagtga attgcagatt gtcctgaaaa
     8401 cggatgatta ccgcataacc atcgattatc tccgaacccg tcctgttgat ttaatcatta
     8461 tggatataga cttgcccgga acagacggtt ttaccttcct gaaaaggatc aaacaaatcc
     8521 agagcacagt gaaagtgtta tttttatcat cgaaatcaga atgcttttat gctggcagag
     8581 cgatacaagc tggtgctaac ggttttgtca gtaaatgcaa tgatcagaat gatatttttc
     8641 atgccgttca gatgatcctc tccggataca cgttttttcc cagcgaaacg cttaactata
     8701 taaaaagcaa taaatgtagt acgaatagtt caacggtcac tgtgctatct aatcgtgaag
     8761 tgaccatatt acgttatcta gttagcggat tatctaataa agaaattgcc gataagttat
     8821 tacttagcaa taaaacagtt agtgcgcata aatctaatat ttatggcaag ctaggtttgc
     8881 attcaattgt agagcttatc gactacgcca aattatacga attaatataa tattaattat
     8941 aattgatcat aaatatcgca tccgctttcg ccacacctgg ccgaacaccg ctggctaacg
     9001 ctcgataatt ggcaaaaaag tttagtgtga cattgccatt tgcatctact tcctcagtcg
     9061 ggctcgcctc ccccagtgcg agccgggagc gatcgctatt acgtaattcg atggcgacgg
     9121 tttgtgccat tgcaggatca tccagagcca gcaggttggc atcagatgcc ggcgttcccg
     9181 taaataaaat cgcaactgaa cccggaggac atccctccag ccgcaggcta aaagggagga
     9241 gtgccgtggt atcgccagcg ttcagtaatt gtgtcgtagg ccatctgcct aaatctaccg
     9301 tcttatcaat atccgctgtg tttacggtac aggagaaatc aacaacgtta ccgtgcaaat
     9361 tgatattaat cgttcctaaa gggtcagctg cccatccact ggaactccac agtaacccgc
     9421 agaaacagct aaagaatact cttctcattc tccgtacctc attgataatc gacgcgtaaa
     9481 taccccagcg cgctaaacgg cccttcggtc ggtttttgac cggtaatact gataggccag
     9541 gcgcgaagtg tgacattggc tgccgcagct gcatccagac ggaaaggaat aacgctattg
     9601 agatcgttag gcgtgatcgg cgtatcgttc tgatcggcga caataaaacc taaatcctga
     9661 ttgtccgaca ccatcgcctg accagaaacg gcactggctt ccagacgcat tgttaaataa
     9721 gcctgcgcag caacattcgt acatttgacc gcaatgctct tggtttgcgg catgacacca
     9781 gcaggccgat tacccggccc tgccgcacta aataacgatg cgccgatatc accaaaatca
     9841 aattcaacaa tctgcccggc atttaattcg cagttttgcg gtacttcaac ccggccacca
     9901 aaactaatgg tataaacagg tgtaaccaac ggatcgtaag gcgttgatgt cacatacacg
     9961 gtaaacatca cctgtctggg gatctccacc atgttaataa agggccgaat aacctttaaa
    10021 cgaaatatca aacgagaatc catcaccccg aacggaaggt tttgcgaaac gttaggatct
    10081 gttcccatac gaatataatt ttgcggggga aattgaatat ccatcacact atcaaccaga
    10141 ctcatcgcac ctaatagata atcatttaat tgcatatatt tataattatc gatagtttcc
    10201 tgaacaataa agttagtgac ataactacgg tatgtataat tcaccagcgt gccgggtgga
    10261 caaattgctg agacacctac ccagccggat ttttccggta aaacaacaat ctgccctggc
    10321 tggttattgg tgctattaaa gacatttgag agatcatagt gttcatcttc tgctacgcca
    10381 gtactatttg cacaaacagt agcccaggat atgtttgcca gacacaaaca tgccatcgcc
    10441 aataataatc tgcagattat cttcattgat aatttccttt atgagccagg atgtgtgcaa
    10501 actgccgaaa taacagtgac ggcttgctgt aagctttgct tcggcaatac atagtgcgca
    10561 atgcagcgtg agttcgcgcc gtctccccac tgaaccagta attcaccaga caatggcgca
    10621 ccacttaaat aaacttgccc gtcatcaccc accatactgg ttattccggt actgttttcc
    10681 cgtaccagtg atccaaacgg caccggtttt ccgccctggg taacggtaat gagcgcccgc
    10741 acgcctatgc gggtatcaaa attggcacga accaacgcgc cttgcgtcgg cacaacgctg
    10801 ctaatatttt tttcaacatc gatggaattc cccatcgtat tggtatcaag cgcgatacgg
    10861 ttataccgat aaaccgtggc atacggcatc accgcatagc cgcgccagtc ggttaaaatg
    10921 ccagtttgat tttcaatgcg tacaccgcct gcgccaggcg ctttaatcaa aacattggta
    10981 tcccctaaag gctggctcag cgttatgcca ttttcatgtc cgaccacacc gccagacagc
    11041 tgccagttaa cgtcatgttg atcgcggtcg tagttatacc cgacccccag cgtaccgtaa
    11101 gcggcctgcc agtttgccgt ggcgctgccc gtatacccat tattcgaggt atcaccctgg
    11161 cttacgtgat aactcaggtt gtggccttcc agtaaggtcc cacctacacc tgccagccag
    11221 ctattttgcc cgttgctgtt acggttggcg ttaaaggagg cataagcgcg gtcgagcgca
    11281 ttttcccggg tgtagcgacg tttggtcaga acattgaaag gcactgaaac attaagtccg
    11341 acaatacgtt cgttatcggg gatccctaca gattcattcc acgaaaatga gagtgaataa
    11401 ctgatgccaa cccagctgct ggtatacccc acctgatacc aggtatctga atccgaagta
    11461 ttccagtatt tttgatgggt accagagata taaagcgagc caaagtcatt aagtgattgt
    11521 gaaatattta actgcaaacg gtctttacgg ctaaagcgta aattgtggta attcacgatt
    11581 atcggttcat cccgatgctc tccgtcataa tcgtaatcat attcgtaccc ctccattcga
    11641 cgatacgcaa catcatcaag ggtataaaaa ccttgtgtcg aatagcggta acccattaac
    11701 tgaaaattgg tgccgaaggt gttcatcgat ttcgcataga ggaagcgaat ggaatccccc
    11761 tcatgacgac tgtcgtcggc taactgactg cgcgcatggg tcacatccag cgacactgcg
    11821 ccccagttcc cgaggttgcg ccccagccct aataaaaagg cggtgtaatt ggcagataat
    11881 tgcgtcccgc cgtaggcagt aaattcctgt ggtaaaccgc cgagtgccgt gccctgaaaa
    11941 aagaaaggcg atgattgctg actattaccg ctacgaaaat cgcccgccgt caggtcaaat
    12001 ttgaaacgcc cttcgcgttg taaaattggc actgttgaat acggaatagt gtaattctgc
    12061 tgattgccat cgcgttcgtc gatggtgacg tccagatcgc catttgaaga tgtcgggtgc
    12121 aaatctgtaa tttcaaaagc gccgggggaa acatagcttt gatagataat aaaaccattt
    12181 tgccgaatcg ttagctgggc cgccgtacgg gcaatcccac gtaccgttgg ggcaaaccct
    12241 tgctggctat caggatacat attatcggaa gaataaagcc gtacaccacg aaatccaaca
    12301 ccatcgaaaa tatcacttcc tgtattgccg tctcccatta ccagttcact tttcagcgga
    12361 ataatggcgc gctgtaccca ggtgccaata ttattccact gttctgaatg atatccattt
    12421 ccgcgaaaat agttccagga accattgttg cgtaaacgcc acgggccaat attaatcccg
    12481 ctgagctcac taaaaaaata gctatcgtta ccattacctc tgttaccggt gaaattataa
    12541 ttcaggagta aagcaggaat accttcatcc cactcttcag gcggaatgta accgtgagcg
    12601 ctactcaaca acgctatctg aggaatagtg atgttcaggc gcatcgctgc aaaatcaaag
    12661 ttaattgtgg catcaggtac agctttcagt aaattgatgc atttattgtt ttgctgctgg
    12721 gctaactcgg gaaatgcact gctattaagg ccaattcgtt caaggagtac ctggttaaaa
    12781 cagggcatta acccacctga tttatcacct gtattttctg ttgtcgattc aaatacgata
    12841 tcctgtgaac caatgaactc atcattacgc cagagatcca cccgatacac gcccgcaggt
    12901 tgatgattac ctctctcaaa gcgcgataaa tcagcaacga atgtgccatt ctctaataaa
    12961 aatgccgggt tgaaataact ttccgcatag cttaaaaagg agggtaaaat ggcaagagat
    13021 gaataacaaa ttccggccag acaccaggta tacctctgcg gaatatccgt agtagtgggt
    13081 attttcataa ttagctctga tattgtcaga tgattcggtt agtttaagtt cacttctctg
    13141 accggggtta ctgaaccata atcattaacg gtctgaaatt tcagctttcc ctgcactcct
    13201 gagggtaagg gaatttgtga attaactctg ggtgcagcca tagcattccc caacttttgg
    13261 ctgcccactt gtaagttaac cagggtgaca taaaaaggtg atggattatg aacattgata
    13321 tagttaccgg atcgcgaaaa cttgagtgta tccggcgctt ctgcgggtaa ttcttgtagt
    13381 tgaattggac ggagaaataa cttcatgcgc gataaaatcg ccagttgcaa aacattcctg
    13441 ccgttcaatg catttttatc taccgaagga atcgtcttaa cattcatcca gaacagagac
    13501 tcacgatctg ctgccagcgg tggaccggtg taaataatac gcaaagtatt ttcgcttttg
    13561 ggttcactaa caaatagtgg cggtgtaatg atgaatgact tttcttttac accgctgctg
    13621 ttttcaatcc acgaattgac cagaaaacgc tcattggtat ggctatttct aatccatacc
    13681 gcagtctgtt tagcatcagc gggataaata atacgcgtgg cacctaatgc gatccctccg
    13741 gcagcatgga cgctggccga aatgattaaa taaaatataa tgagcatcaa taactttatt
    13801 ttagtcatca tagttaatac gccttgtgta ttcctgacgg caaaaataag aaatataaag
    13861 aaatgattgc tttggtttct tacaacacat cattcccttt tcatttaata aagcggcatg
    13921 atacaaagtg aacatgattt cgatttacat tgccaccata ccgcttcaga caaccggtac
    13981 attactttgt tataagacgt gatatataat tcaaaacaac gtggttttga ttattcgtat
    14041 ctcataataa aagtcgcgtc agcatttgct tgccctgctg atgcacttgt accggtgcct
    14101 ttataacgtg cagaaaaatg aagaacgttg gtcccgggga tcaagttctg gttggttgag
    14161 aagctattcc catcaggttt gagaattgcg gatgtattat caagtatttc aataccgaca
    14221 cccgttgctg ttgttgtatt ggtactggat gcaatggcca ataaattatc attgattgca
    14281 tcagcctgac cagaaaatgc aacggcagca ttagcggcaa caacaggatc gcagtcgttc
    14341 aactgaatgg tgaatggaat taatgcagag gtattaccaa cagcattgaa aatatcggta
    14401 cgatattgac cgagtgtgac aacctggtct gccgagtcag tattcactga acaggcagca
    14461 ttcaccagtt caccttcaaa gtgaattgta ccgccatcta caacggcagc ataactaccc
    14521 gtagcggcga atagtgcggc agccagcgca gacgaaataa atcttaattt catatatatt
    14581 ccttcaatct catttatcga ctccacatcc gtatataacc gattacttta tttaagacac
    14641 tgatagtagt aaattccttt ttatcctcta agaatgtctt aattgaaaat atgcactcta
    14701 ttctaaaaaa tagagagccc cgttagatga atacttccgc gcaaaatata ttcaacacaa
    14761 atatagacct gaagcggtaa attaccaggc tgaaaattct ttttatattg tcaggtattt
    14821 cttaaattat cttaatcctt aaacaaggaa ataaatcagt tccagattta caacgccatc
    14881 atggacgaaa aatgaagctt tcaatctcag cgacctcagc gacagtgcgc ctaaccttcg
    14941 caagaggtcg cttcacgcga taaatctgaa acgaaacctg acagcgcgcc ccgcttctga
    15001 caaaataggc gcatcccctt cgacctacgt aacagatgga atcctctctc tgatggcagc
    15061 aaagattatt gacggtaaaa cgattgcgca gcaggtgcgc tctgaagttg ctcaaaaagt
    15121 tcaggcgcgt attgcagccg gactgcgggc accaggactg gccgttgtgc tggtgggtag
    15181 taaccctgca tcgcaaattt atgtcgcaag caaacgtaag gcttgtgaag aagtcgggtt
    15241 cgtctcccgc tcttatgacc tcccggaaac caccagcgaa gcggagctgc tggagcttat
    15301 cgatgcgctg aatgccgaca acaccatcga tggcattctg gttcaactgc cgttaccggc
    15361 gggtattgat aacgtcaaag tgctggaacg tatacacccg gacaaagacg tagacggttt
    15421 ccatccttat aacgtcggtc gtctgtgcca gcgcgcgccg cgtctgcgtc cctgcacccc
    15481 gcgcggtatt gtcacgctgc ttgagcgtta caacattgat accttcggcc tcaacgccgt
    15541 ggtgattggc gcatcgaata tcgttggccg cccgatgagc atggaactgt tgctcgcagg
    15601 ttgcaccacc accgtgactc accgtttcac taaaaatctg cgtcaccacg tagaaaatgc
    15661 cgatctgttg atcgttgccg tcggcaagcc gggctttatt cctggtgact ggatcaaaga
    15721 aggcgcaatt gtgattgatg tcggcatcaa ccgtctggaa aatggcaaag ttgtgggcga
    15781 cgtcgtgttt gaagacgcgg ctaaacgcgc ctcatacatt acgcctgttc cgggcggcgt
    15841 tggcccgatg acggttgcca cgctgattga aaacacgcta caggcgtgcg ttgaatatca
    15901 tgatccacag ggtgagtaac atggcgacat tttctttagg taaacatccg cacgttgagc
    15961 tgtgcgactt gctgaaactg gaaggctgga gcgaaagcgg cgcgcaggcg aaaatcgcga
    16021 ttgccgaagg ccaggtgaaa gtcgacggcg cggttgaaac gcgcaaacgc tgcaaaatcg
    16081 tcgccgggca gacagtgagt tttgcaggtc acagcgtaca ggttgttgcc tgatcccttt
    16141 cccggcctgt atcaacaggc cggttctctt ttcagacttt tgccctctac gccccgttca
    16201 tccatgctga tagcatcgac attttgccga agcgaccatc atgccaaccg ttattacgca
    16261 cgccgccgtt cccctatgca ttggtttagg gttgggttcg aaagtcatcc ctccgcgcct
    16321 gttatttgcc ggaatcatcc tggcgatgct gcccgatgcc gacgtcttgt cgtttaattt
    16381 tggcgttgct tacggcaatg tttttggtca tcgcgggttt acccattcgc tggtgtttgc
    16441 gtttgttgtc ccgctgttat gtgtgctgat tggacgtcga tggttcaggg ctgggctgat
    16501 tcgctgctgg ctgtttttaa ccgtctcgtt gctatcgcac agcttgctgg attcggtaac
    16561 cactggcggt aaaggggttg gctggctgtg gccgtggtca gatgaacgct ttttcgctcc
    16621 ctggcaggtg attaaagtcg cgccgtttgc attgtctcgt tacaccacag cgtacgggca
    16681 tcaggtgatt atttctgaat tgatgtgggt gtggttgccg gggatgctac tgatgggaat
    16741 gttgtggtgg cgcagacgat aaccggatgc gaaaactcgc atccggcaat agcgcaatta
    16801 cttacgacgc caggtggtcc cttgcgggcc atcttccagc acgatcccca tctcattaag
    16861 acggtcacgc gccgcatctg ccgccgccca gtctttcgct ttacgggcat ccagacgctg
    16921 ttgaattaac gcttcaatct cagccacttc gctgtcgtct gcctgcgcgc cgctttgcag
    16981 gaacgcttcc ggttcttgct ccagcaggcc caatacggcg gaaagtttac gcaggtgaga
    17041 tgccattgca ttcgccgctg ccatatcttc tactttcaga cggtttactt cacgcgccat
    17101 atcaaacagc acggaatagg cttccggggt gttgaaatcg tcgtccatcg cttcaataaa
    17161 gcgcgcttca aacgcttcgc caccggcagg cgcaacggtt ttatctgtgc cgcgcagcgc
    17221 agtgtagaga cgctccagcg ccgcacgcgc ctgcttcagg ttctcttcgc tatagttcag
    17281 ctggctgcga tagtggcccg acatcaggaa gtaacgcacg gtttccgcgt cgtagtattt
    17341 cagcacatcg cgcacggtaa agaagttacc cagcgatttg gacatcttct cgcggtcaac
    17401 catcaccatc cccgagtgca tccagtagtt cacatactga ccatcatggg cacaggtgga
    17461 ctgcgcgatt tcgttttcgt ggtgcgggaa catcaggtct gaaccgccgc cgtggatatc
    17521 aaagtggtta cccagctgct tgcagttcat tgccgaacat tcaatgtgcc agccaggacg
    17581 acccgcgccc cacggagacg gccagctcgg ttcgccctct ttcgacatct tccacagaac
    17641 gaagtccatt gggttgcgtt tgtcgtcgac cacgtcaacg cgcgcgcctg cctgcagctg
    17701 gtcgagatcc tgacgcgaca gcacgccata agttggatcg gtcgggacgt cgaacatcac
    17761 gtcgccgttg tccgccacat aagcgtgacc tttggcgatc agttgttcag tgagttcaat
    17821 aatttctgcg atatggtgcg tcgcgcgcgg ctccatatcc gggcgcagaa tgttcaacgc
    17881 atcaaaatct ttgtgcattt cggcgatcat gcgatccacc agcgccacaa agctttcgcc
    17941 attttcattg gcgcgtttga tgattttgtc gtcgatatcg gtaatgttgc gcacatactt
    18001 cagcttatag ccgaggaaac gcagatagcg cgcaaccacg tcaaaagcaa caaaggtacg
    18061 cccgtgaccg atatgacaga gatcgtaaac ggtgattcca cacacgtaca tgccgacttc
    18121 cccggcgtga ataggcttaa attcctcttt ttggcgtgtc agagtattga agatttttag
    18181 catcgaagat tccgtttaga catgtgtggg taattgagtt gcgtataata cccatatttc
    18241 ccgcgcgaat cagcatacat tgcgtgatga tccgatcgtg cggttatgct ataacaccac
    18301 cctatatatg acccgaactg ggttgaagca ccaatcaaac ggaacaggat gcaaaaatgg
    18361 ttactttcca caccaatcac ggcgatattg tcatcaaaac ttttgacgat aaagcacctg
    18421 aaacagttaa aaacttcctg gactactgcc gcgaaggttt ttacaacaac accattttcc
    18481 accgtgttat caacggcttt atgattcagg gcggcggttt tgaaccgggc atgaaacaaa
    18541 aagccaccaa agaaccgatc aaaaacgaag ccaacaacgg cctgaaaaat acccgtggta
    18601 cgctggcaat ggcccgtact caggctccgc actctgcaac tgcacagttc ttcatcaacg
    18661 tggttgataa cgacttcctg aacttctctg gcgaaagcct gcaaggttgg ggctactgcg
    18721 tgtttgctga agtggttgaa ggcatggacg tggtagacaa aatcaaaggt gttgcaaccg
    18781 gtcgtagcgg tatgcaccag gacgtgccaa aagaagacgt tatcattgaa agcgtgaccg
    18841 ttagcgagta atcgtggcga cactctttat tgcagatctt catctctgcg tggaagaacc
    18901 ggcgatcacc gccggttttc tgcgtttttt agcgggagaa gcccgcaagg ccgacgcgct
    18961 gtatattctt ggcgatctgt ttgaagcatg gattggcgac gacgatccca acccactcca
    19021 tcgccagatg gcggcagcga tcaaagcggt gtccgattcc ggcgttccct gttatttcat
    19081 tcatggcaac cgtgattttc tgctcggcaa acgctttgcc cgtgaaagtg gcatgacgtt
    19141 attgccggaa gaaaaagtgc tcgaacttta tggtcgtcgg gtgctgatta tgcatggcga
    19201 cacgctgtgc accgatgacg cgggttatca ggcttttcgc accaaagtcc acaaaccctg
    19261 gctgcaaacg ctattcctcg ctctgccgtt gtttgtgcgc aaacgcattg ccgtgcgaat
    19321 gcgcgcgaac agtaaagaag ccaacagcag taaatcgctg gcgatcatgg acgttaacca
    19381 aaacgcggta gtcagtgcga tggaaaaaca tcaggtgcaa tggctgatcc acgggcatac
    19441 ccatcgcccg gcggtgcatg aacttatcgc caatcagcaa cctgcttttc gcgtggtact
    19501 gggtgcctgg catacggaag gttcgatggt gaaagtcacg gcggatgacg ttgagctgat
    19561 tcattttccg ttttaaaaaa cccgcaactt tgctgatttc acagccacgc aaccgttttc
    19621 cttgctctct ttccgtgcta ttctctgtgc cctctaaagc cgagagttgt gcaccacagg
    19681 agttttaaga cgcatgtctt cccgcaataa tccggcgcgt gtcgccatcg tgatggggtc
    19741 caaaagcgac tgggctacca tgcagttcgc cgccgaaatc ttcgaaatcc tgaatgtccc
    19801 gcaccacgtt gaagtggttt ctgctcaccg cacccccgat aaactattca gcttcgccga
    19861 aagtgccgaa gagaacggtt atcaggtgat tattgcgggc gcaggcggcg cagcgcacct
    19921 gccaggcatg attgccgcca aaacgctggt gccagtgctg ggcgtgccag tacagagcgc
    19981 cgcactgagc ggtgtcgata gcctctactc catcgtacaa atgccacgcg gcattccggt
    20041 gggtacgctg gcgattggta aagctggcgc ggcaaacgcg gcattactgg cagcacaaat
    20101 tcttgcgact catgataaag aactgcacca gcgtctgaat gactggcgca aagcacaaac
    20161 tgatgaagtg ctggaaaacc cggacccgcg aggtgcggca tgaaacaggt ttgcgtcctc
    20221 ggtaacgggc agttaggccg tatgctgcgt caggcaggcg aaccgttagg cattgctgtc
    20281 tggccggtcg ggctggacgc tgaaccggcg gcggtgcctt ttcaacaaag cgtgattacc
    20341 gctgagatcg aacgctggcc ggaaacagca ttaacccgcg agctggcgcg ccatccggcc
    20401 tttgtgaacc gcgatgtgtt cccgattatt gctgaccgtc tgactcagaa gcagcttttc
    20461 gataagctcc acctgccgac tgcaccgtgg cagttacttg ccgaacgcag cgagtggcct
    20521 gcggtgtttg agcgtttagg tgagctggcg attgttaagc gtcgcactgg tggctatgac
    20581 ggacgcggtc aatggcgttt acgcgccgat gaaaccgagc agttaccggc tgaatgctac
    20641 ggcgaatgta ttgtcgagca gggcataaac ttctctggtg aagtgtcgct ggttggcgcg
    20701 cgctgctttg atggcagcac cgtgttttat ccgctgacgc ataacctgca tcaggacggt
    20761 attttgcgca ccagcgtcgc ttttccgcag gccaatgcgc agcagcaagc gcaagccgaa
    20821 gagatgctgt cggcgattat gcaggagctg ggctatgtgg gcgtgatggc gatggagtgt
    20881 tttgtcaccc cgcaaggtct gctgatcaac gaactggcac cgcgtgtgca taacagcggt
    20941 cactggacac aaaacggtgc cagcatcagc cagtttgagc tacatctgcg ggcgattacc
    21001 gatctgccgt taccgcaacc agtggtgaat aatccgtcgg tgatgatcaa tctgattggt
    21061 agcgatgtga attatgactg gctgaaactg ccgctggtgc atctgcactg gtacgacaaa
    21121 gaagtccgtc cgggccgtaa agtggggcat ctgaatttga ccgacagcga cacatcgcgt
    21181 ctgtccgcga cgctggaagc cttgatcccg ctgctgccgc cggaatatgc cagcggcgtg
    21241 atgtgggcgc agagtaagtt ctgttaacct ggtgctcatt tgattgccgg atgcggcgtg
    21301 aacgccttat ccggcctaca ccgatccggt acccattgta ggcctgataa gacgcgtcaa
    21361 gcgtcgcatc aggcatggtg cgccgattgc cggatgctct catcactaca gcgaaataca
    21421 ggtccccgct tcgcccgcca gcgtctcttc aattcgcgat aacgccccaa tccacgcggg
    21481 tttaccacgg cttctgacat aaccactcac cgccgttacc ttcggcccca tcgaaccatc
    21541 ggctttggca aatggcgcta actcatccgg tgtggcatgg cgaatggcac gttgctgcgg
    21601 cattccccag ttttcatata ccgcatcagc atcggtgagg atcaccagtc catcggcgtt
    21661 aatttgctcg gcgagcaacg cagcggcgag atctttatca atcactgctt cactccctgc
    21721 tccgtcatcc gtcacaggca caccgccgcc gccactgcaa atcaccacat gcccctcttt
    21781 gagcaacaac tcgatggctt cgctgtcgag aattttacgc ggttgcggag acgccaccac
    21841 ccggcgcaaa tatttaccat cacgtttcat ctgccagccg taagccgctt ccagtgcctc
    21901 ttgttcttct ggctgataaa ccggaccaat aaatttctct ggctgcaaaa acgccggatc
    21961 atcaggcgaa acttcaatgc gcgtccgcac cgtcgtgaca ggcggcatct gcggctgtgc
    22021 gctcaaactc tgcgccagca tatagccaat catcccctga ctttctgcca ccagcacatc
    22081 cagcggatac ggttctacct ctttccacgc cagattctgc aatgccagca gccccacctg
    22141 cggcccgttg ccgtgaacaa tcgccagccg ataagaacgg gccaggcgcg ccagcgcggg
    22201 tacagcactg gcgatattgc gatattgatt ttctgccgtc agcgcctcac cgcgctggag
    22261 taaggcgttg cccccaagag ctacaaccag tgttttcatg gttttccttg taataattgt
    22321 tggccaagcc agaaccccag cagtgtatct gcccctgacg tatgccccag cgccagcagc
    22381 gaatcaatcg caacagcggt acgtttcggg caactcagag catgaacaaa gtgcaggagt
    22441 ggcgaggcga aatatccttg cgcggcataa cgtaaataac tgacgctcac cgctgtggta
    22501 acgagttgca gattgtcgga acaggcaaaa aacggacgac cggagcgcgc atctaaagca
    22561 ccataatacc aggccgccag cagcattccg ctcagcgtgt catcatgact tggcgttaat
    22621 ccggggcctt tacccagcca gtgccgccag tcggtcttaa cgccattgag cgcggcctga
    22681 aaacagtgac gaaactggcg taactcagca ggcagcggat cgcttgccgc caacgccagt
    22741 ggcccgaaaa gcccggtttc ctccgcgcgt tgcatccatg cagctgcaag tggttgagga
    22801 tgcgcaggcg gcgtaatacg cagcaaacaa taacgctgtg gctgtttaac cgtgaaacgc
    22861 ccgaggcgaa tcccttgagc cacaacctgt ggtcgttcat tgccgcataa tccaccgcat
    22921 aacgcatcga attgcgcacg gcgaagcacc catcctccgg ggccgaaacc actaccctga
    22981 cgatgcagcg ttaacagttc gccgctttcc gtcatcaggt taatcgcccg ccgccacact
    23041 cccgctaacc gccaggactg gcgataattc ggtgcgctac tactggcaag cagaggatgg
    23101 atgatcgtca ttacgcgttc ctcaaccaat ccccatgctt tcagccagtg cttccagcgc
    23161 ctgttcaaag cacgccagcg gtgcccgcac ggtgcctgcg ccaatctgcc cgatccccgc
    23221 ctctttatgg gcgataccgg tattgatgag tggcgtaatg ccggtttcta ccacgcgacg
    23281 aatgtccagt cccaggcacg cgccctgaaa atcccagctt gggatctgca actgcatatt
    23341 gcgttcaagg taaatttccg ccatctcttc agataccgcc ctggccgctt ccatgccact
    23401 cgcaccgaca aagcgcgtta cgccaggcgc tgcgatcatt gccgcacctc cgataccaaa
    23461 ggtttcggta atcgcgctat cgcccatatc cgggttcgcc tgctcctgcg agaagccggt
    23521 gaaaaacaga ccttgcggag tgtttacagg cgcagtaaac cagcgttcgc ccagcccgct
    23581 tacccgaatc ccgaacatat tgccgttgcg ggtcattgcc gtgacgatgc tgcctgcgcg
    23641 gatcatcgcg ccagcatcca tcgccgcctt gcagtaagcc atcgcgaggt tgaggaagaa
    23701 ctgatcggtc acgctgagga aatccatcac ttccgcgatg tgctgtttat catgatcgag
    23761 gcgagcaatt tgtggggcca gcgcacgcat taacagtgct gaggaagcaa tattgcgttg
    23821 atggaactca tcgcccatcg taatgccctg cgccatcatc gcagtaagat cgataccgcg
    23881 ctccatgcgc cccagcgccg cgcttaatac tggcattaac acatcgcgca tccagcgatg
    23941 gcgagtcagg acatcttcgc cgtaagcgcc aaaacgcatc actttgccga taccttcgtt
    24001 gaggttgcag tacgcccggt tgccgtcggt cacgttctcg accaccagca tcggcatact
    24061 ggcagaagta ataccgccca ttggcccgac ggcattcaca tggtgacaag gaatgaagtt
    24121 cacttccccc tgctccagta ttgccagcgc ctgcgcttca tctttcgccc aaccttcgaa
    24181 cagacatgcg cccacgcacg cccctttcat gggtccggtc atctcctgcc agcgcattgg
    24241 cggcccggcg tgaagcagtg ttttgccctc gtttagttcg ctgataagtg aagaagccgg
    24301 ttgcacatcc agccagtgtg gacgagcgcg acgaatttgt tcgataaccg cagcattggc
    24361 ttgcgccact gatgtaaaca tgagaacccc ttattgcaaa cgttctaata aacgagccag
    24421 ttttttattg ccgccggcga ctggcgacca ttggtaatgc acaaccggtt tgctggcgct
    24481 ttgtagctcc agcgcaaaac tgcgtaatcc gatgttaatc acggcgacgt tttccagtaa
    24541 tgacggtgtg tgttgctgtg cggcaggcga gagcggatga attaacgctg ccgccagcaa
    24601 ggtggcttcc ggtagcgaac tcacgaccgc aatccccgca tcttccagcg tggcgatttg
    24661 ctgcgagcgg cattgcgggt cacgttcagt gcctgtcacc gtggcaatgg catacagtgg
    24721 ttgattgtct gaacgcgcag cacaggcttt ttgccaggcg cttaccagcg aggcggcagg
    24781 atcggcggtc gcaccgaagc caatcacgac atcaagcagc agcacacgca cttgcggttt
    24841 agcgccgaga tcggtaatta actggttgcg taaggttggg tcgatcatcg gatggggacg
    24901 cccgacggtg tagaaatcat cgccgaggtc aataatctgg tggccatcgg cgtccagcat
    24961 catgccgtga tgatgggcat catcggcttc cacgccaagg tgtccggcaa gtaatcccgc
    25021 cgcttcggca gccagcgtac cgccggtata caaaccgcaa ataaatccgc tgctggcagg
    25081 cattatcgcg ttacgtcgcg ccgtaacgcg tgaaagcagg caagccaggc gtgcggcctc
    25141 atccagcgag gaggcaaacc agacattctc gtcgcgggcc accgccgggg tataacctaa
    25201 aaacagcgcc accgtcggtt tgccggttgc tttcatggca ttaacaattt tcagacgcac
    25261 cgcttcggca ggtggttttg aaacaaatgc cagcacttcg cttttctcgt ctgcactgag
    25321 catttccagc gccgtcagcg cactgatgcc gcccacttca cggctgaggt cgcggccgcc
    25381 aaggccaatc gcgtgagtaa ttccctcccc tgccagcgca atctgcgagc acagctcctg
    25441 aatcccggta ccggaagcgc caatgacgcc aatgttgcct tccggcatca cgttagcaaa
    25501 agccagcggc gtgccggcaa tcatcgacgt accgcagtcc ggccccatca ccagcaagcc
    25561 tttttcccgc gcgcgggttt taagttggat ttcatcttcc agcgtgacgt tatcggagaa
    25621 catcatcacg ttgaggttgc gatccagcgc ctgattcgcc agctccgccg catactcgcc
    25681 agccactgaa atcagcgcca gattggcatc gggtaatttc tgacaggcac tgtcccagcg
    25741 acgcacctgc gtcaacgcct ggctgctgcc tgacccctgc gccagttgtt ttagcgcctc
    25801 ttcaagctgc tgcataatcg cctgcgcgat ccccgcatcc gccgcttcgc tacgaattgc
    25861 cacgcaaata tcgttcggcg tggcgttatt aaaatcgtca tgccagaaac ctgtggtatc
    25921 taataacgct ttattggcgg gcgtacccat cattacggaa acatcatcaa cattttctga
    25981 ttcgctgagt tttcgtgaaa taatcattaa actgaccgaa tcctgaaaac accctttttt
    26041 aataaaggcg tggatcataa caactcctta aaaattgcac ccatggcaat tgcacgagca
    26101 gtgttaatgt ggcgattacg gtatcgatga caacgagtaa ataaggtgat gaatatcaca
    26161 cagggattat taacaa
//
LOCUS       DAZKIP010000057        26143 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZKIP010000057 DAZKIP010000000
VERSION     DAZKIP010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26143
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.057"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(511..1182)
                     /gene="queE"
                     /locus_tag="ACHOUK_002605"
     CDS             complement(511..1182)
                     /gene="queE"
                     /locus_tag="ACHOUK_002605"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFP9898023.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     gene            1337..1957
                     /locus_tag="ACHOUK_002606"
     CDS             1337..1957
                     /locus_tag="ACHOUK_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HFP9898024.1"
                     /translation="MKRDLNTLRIFIVIIFIFNLSGCGYNDIQTYDEQVNASWSEVLN
                     QYQRRADLIPNLVASIKGYSSHERDVLEAVTLARSQANRASSDLQQTPGDEQKLQAWQ
                     QAQAQVTRTLGQLTIISERYPELKSQELYQNLMVQLEGSENRIAVARGRYIKAIEQYN
                     VTIRKFPAVLTAKVMDYTPKKNYLPDDVAAVSKAPTIDFSQNANAH"
     gene            1971..2645
                     /locus_tag="ACHOUK_002607"
     CDS             1971..2645
                     /locus_tag="ACHOUK_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFP9898025.1"
                     /translation="MRNFIFLMAFFCSSVFATQIPVPESPKYVNDLTGTLTNSEVNTL
                     TNQIKALTQKNYAQLVVLVVETTGDETIEQYATRVFDSWKPGDKDRDDGVLLLVAWQD
                     HTVRIEIGYGLEGIITDAQSGKIIRNSIIPAFKKGDLAGGLQKGISDIESRLTGNNLA
                     TITPTDHPLPFSGWWALLVWAIVLTFISARGYIKTLGVICFAAIVLAFVLPIAGFSGS
                     WGVLAT"
     gene            2879..4027
                     /locus_tag="ACHOUK_002608"
     CDS             2879..4027
                     /locus_tag="ACHOUK_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFP9898026.1"
                     /translation="MRIFLLLLMLCGFQVFAERLPSVTDLTGTLTTEEQTALTQQLQT
                     LEQQNHFQVAVLVTPTTGGKNIKLAASQRYGVYHWKPVGEKRYGEGILILVVWPEGLA
                     SMKIGHGLEEMLPPEQAAQIVRYHMQPEFEKNNLFAGLTGGIESIAQFTHIKANLSPL
                     DALANHLFANPQRSLPCLAWTLLMIVAIVVLWLFASRPRRGIWMISMITPMVWFFSFQ
                     DDVIIRRVSVVCFSLLLATTCKQRLAVVFNMCRVFPMMLTPKNKNAKVKKQKKTPQVR
                     ESGRNSLFVVMLIIIVGWCLVFASNKDIAFISTIIGLIDHFIGPITIAGIVLLIIVAK
                     FTGNLKLGSGEKSNRKTGNRNAHSRSSSRNSFRGGGGSSGGGGSSVRW"
     gene            4024..4938
                     /locus_tag="ACHOUK_002609"
     CDS             4024..4938
                     /locus_tag="ACHOUK_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311665.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFP9898027.1"
                     /translation="MIVMRLIFILFTLWCLPGLAQQIAVPELRQQVTDITGTLSTSEQ
                     QSLTQQLQDITHKTRAQVAVLVVPSTGDDSIEQYATRVFDSWKLGDKQRNDGILLLVA
                     WEDHAVRIEVGYGLEGVVTDLQAAKIIRDILIPAFKSDDLMGGLTLASENIGALLLNG
                     ELPEDRGDYYSINPPIPLSLAVIILLAVLSYFIVFTDPSNLPWITLTGAIYGMVFLYV
                     AEPGPWTNLIVACGMLTPFAIVPLVIFWLIVNKKLRAKYKKLSKDRASRKGSSSSSSG
                     GGSSGGGSGGGFSGGGGSSGGGGASGRW"
     gene            complement(4998..6296)
                     /gene="eno"
                     /locus_tag="ACHOUK_002610"
     CDS             complement(4998..6296)
                     /gene="eno"
                     /locus_tag="ACHOUK_002610"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFP9898028.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(6384..8021)
                     /gene="pyrG"
                     /locus_tag="ACHOUK_002611"
     CDS             complement(6384..8021)
                     /gene="pyrG"
                     /locus_tag="ACHOUK_002611"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFP9898029.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(8249..9040)
                     /gene="mazG"
                     /locus_tag="ACHOUK_002612"
     CDS             complement(8249..9040)
                     /gene="mazG"
                     /locus_tag="ACHOUK_002612"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFP9898030.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(9111..9446)
                     /gene="mazF"
                     /locus_tag="ACHOUK_002613"
     CDS             complement(9111..9446)
                     /gene="mazF"
                     /locus_tag="ACHOUK_002613"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFP9898031.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(9446..9694)
                     /gene="mazE"
                     /locus_tag="ACHOUK_002614"
     CDS             complement(9446..9694)
                     /gene="mazE"
                     /locus_tag="ACHOUK_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFP9898032.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(9772..12006)
                     /gene="relA"
                     /locus_tag="ACHOUK_002615"
     CDS             complement(9772..12006)
                     /gene="relA"
                     /locus_tag="ACHOUK_002615"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFP9898033.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(12054..13355)
                     /gene="rlmD"
                     /locus_tag="ACHOUK_002616"
     CDS             complement(12054..13355)
                     /gene="rlmD"
                     /locus_tag="ACHOUK_002616"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFP9898034.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLEVEPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNCLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            13412..16168
                     /gene="barA"
                     /locus_tag="ACHOUK_002617"
     CDS             13412..16168
                     /gene="barA"
                     /locus_tag="ACHOUK_002617"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFP9898035.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGCGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(16401..17741)
                     /gene="gudD"
                     /locus_tag="ACHOUK_002618"
     CDS             complement(16401..17741)
                     /gene="gudD"
                     /locus_tag="ACHOUK_002618"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFP9898036.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLLTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(17762..19102)
                     /gene="gudX"
                     /locus_tag="ACHOUK_002619"
                     /pseudo
     CDS             complement(17762..19102)
                     /gene="gudX"
                     /locus_tag="ACHOUK_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
     gene            complement(19104..20456)
                     /gene="gudP"
                     /locus_tag="ACHOUK_002620"
     CDS             complement(19104..20456)
                     /gene="gudP"
                     /locus_tag="ACHOUK_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFP9898037.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIAISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVVGQ
                     "
     gene            complement(20891..21340)
                     /gene="yqcA"
                     /locus_tag="ACHOUK_002621"
     CDS             complement(20891..21340)
                     /gene="yqcA"
                     /locus_tag="ACHOUK_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFP9898038.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(21358..22140)
                     /gene="truC"
                     /locus_tag="ACHOUK_002622"
     CDS             complement(21358..22140)
                     /gene="truC"
                     /locus_tag="ACHOUK_002622"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFP9898039.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGERSS"
     gene            complement(22140..22469)
                     /gene="yqcC"
                     /locus_tag="ACHOUK_002623"
     CDS             complement(22140..22469)
                     /gene="yqcC"
                     /locus_tag="ACHOUK_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFP9898040.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(23091..23636)
                     /gene="syd"
                     /locus_tag="ACHOUK_002624"
     CDS             complement(23091..23636)
                     /gene="syd"
                     /locus_tag="ACHOUK_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFP9898041.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLAPNLAEFLNQLKPLL"
     gene            23704..24552
                     /gene="queF"
                     /locus_tag="ACHOUK_002625"
     CDS             23704..24552
                     /gene="queF"
                     /locus_tag="ACHOUK_002625"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311681.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFP9898042.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFQGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGEVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            24664..26028
                     /gene="ppnN"
                     /locus_tag="ACHOUK_002626"
     CDS             24664..26028
                     /gene="ppnN"
                     /locus_tag="ACHOUK_002626"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFP9898043.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         6496 a         6810 c         6226 g         6611 t
ORIGIN      
        1 catgaggggt tacgtggata tgttgcttat tacgagtact gctaatataa aaacttgaga
       61 aagagataac gggttatatg gtggtttatc cccactggcg cggggaactc ctcgacttta
      121 attttttcgc tgcccgcttt tgcggtttat ccccgctggc gcggggaact ctctaaacat
      181 aacctattat taattaatga ttttttaagc cagtcacaat ctaccaactt tacagtatca
      241 cacaaacaac acatccatta tgttaaagag cacttaaccc attgattaaa aaggtaaata
      301 tttaaaataa ctctatacaa actaaaatct accaaacttt accgcaataa ttttcactcc
      361 agcgaaaaat taacgccaca gaatttgtag agaatgataa tgattagtcc gggataggca
      421 agatggatga gttttcagag acgacaaaga atataaatga gtagaagtct ttacaggttc
      481 cacccattta taacgcttat aaatgtttaa tcaggcaata tttaaatatt tatgggtttg
      541 catcgacaaa cgccaattac gcgcaatgca ggtttcaatg cacaaacgtg tggcatcatc
      601 cttttggcta atcggctgca gtgcaatgac tcgcggttta tcatcggtca gcgtcgccaa
      661 aagttcatcc agtgcttcaa tatcacgtac gcgtcccact ggatgcttga tttcgttggc
      721 tcgctccagt gcctgtgaca acacttcata gccgccgcgc atgttcagct ttggcgatac
      781 ggtaacccag gtattcggtg tacagcgtac ttcatgagta ccactggttt cgatctggca
      841 gctaaaaccg ttcttttcga gcaggtcagt cagtggcagc aaatcatgaa tgcaaggctc
      901 accacccgta atcaccacat gccgcgcggt atatccctgg cgaccaatga cagccagcaa
      961 gtcttcactg ctcgccgccc cccacttatc actctctttg gtcttcgcca gaatgctgaa
     1021 aagggagact tcccgatcct caagcttttc ccaggtgtgt ttggtgtcgc accaggcaca
     1081 gccaaccggg catccctgta aacgaataaa aatggcgggg acgccggtaa agtaaccctc
     1141 accttgcagg gtctggaaca tctcgttaat cgggtactgc atagcattct ctgtgaagtg
     1201 gataattgtt aattattgca gatcctgcca caacaatcat gtcttattaa cattctgtta
     1261 caggcaggtt tatggcaaaa agtggattag atcataaaga ataaaatgaa gcagagtatt
     1321 atgttttaag gaattaatta aaagggattt aaacacgctc agaatattca tcgtaattat
     1381 atttattttc aatttatcag gctgtggata taacgatatt caaacctatg atgagcaggt
     1441 taacgcctcc tggtctgagg tattgaatca atatcaacgt cgcgccgatc ttattcctaa
     1501 tcttgtcgcc agcattaaag gttattccag ccatgaacga gatgtactgg aagccgtaac
     1561 attggctcgt agccaggcta atcgcgccag tagcgatctt cagcaaactc cgggagatga
     1621 acaaaaatta caagcctggc aacaggctca agcgcaggtg actcgcactc tcggtcagtt
     1681 aacgataatt agtgagcgtt accctgagtt aaaatctcag gaactctatc aaaatttaat
     1741 ggtacagctt gaaggaagtg aaaatcggat tgccgttgcg cgaggaagat atattaaagc
     1801 cattgaacaa tataatgtca ctatccgcaa attccccgct gtattaaccg caaaggtcat
     1861 ggattacaca ccaaagaaaa attatttgcc ggatgatgtc gccgcagtaa gtaaagcccc
     1921 aacaatagat tttagccaga atgctaatgc tcattaagcg gggaagttct atgcgaaatt
     1981 ttatcttcct tatggctttc ttctgttcat ctgtgtttgc cacacaaatt ccagtacctg
     2041 aatcacccaa gtatgtgaat gacttaaccg gcacattaac aaacagcgaa gtaaacaccc
     2101 tgaccaacca gattaaagcc ctgacgcaaa aaaactacgc acagttggtc gtgttagtgg
     2161 ttgaaacaac aggcgatgaa accatcgagc agtacgcgac gcgggtattc gatagctgga
     2221 aaccgggtga taaagaccgc gatgatggcg ttctactgtt agtcgcctgg caggatcata
     2281 ccgtgcgtat tgaaatcggt tacggactgg aaggtattat tacggatgcg cagtccggga
     2341 aaattattcg caacagtatt attcctgcat ttaaaaaagg cgacctggct ggtggtttgc
     2401 aaaagggaat cagcgatatt gaaagtcgtc tgacggggaa taatttagca acgataacac
     2461 cgaccgatca ccctttgccc tttagcggtt ggtgggcatt gttagtctgg gcgatagtac
     2521 tgacgtttat ttctgccaga ggatatatca aaacgctggg agtaatttgc ttcgcagcta
     2581 ttgtactggc ctttgtttta cctatagcag gcttcagcgg aagctgggga gtactcgcaa
     2641 catgactctg cttcgccaca ccgttcctgg ctgtcgccat cgcttttacc cccttcggca
     2701 aaaaagttcg ggatttgatg cgtgatgcca accagccatc acgccataca agaagcaatt
     2761 cttcctccca ctattcatca tcgtcatcct cttcattcga caatgacaat tttagcggtg
     2821 gtggcggttc atctggtggc ggtggcgctt ccgggcgctg gtaacaaaag gaattgatat
     2881 gcgtattttt ctcttacttc tgatgctatg cggttttcag gtattcgctg aacgattgcc
     2941 ttcagtgact gacttaaccg ggacattaac gacagaagaa caaactgctt taactcaaca
     3001 attacagact ctcgagcagc aaaaccactt ccaggtggcc gtgttggtta cgccgaccac
     3061 agggggcaaa aacattaaac ttgcagcctc gcagagatac ggggtatatc actggaaacc
     3121 cgttggagag aagcgatatg gcgaaggtat cttaattctc gtcgtatggc cggaagggct
     3181 ggcgtcaatg aagattggac atggactgga agagatgttg ccgccggaac aagctgcgca
     3241 aattgttcgt taccatatgc agccagaatt cgaaaaaaac aatctctttg ctggattaac
     3301 gggcggaatt gaaagcatcg cacagtttac ccatatcaaa gcgaacctta gccctttgga
     3361 tgctttagcc aatcatctgt ttgctaatcc gcaacgttcg ctgccatgtc ttgcctggac
     3421 actgctgatg atcgttgcca tcgttgtcct ctggctattt gccagccgcc cacggcgtgg
     3481 catatggatg atatcgatga ttacccctat ggtctggttt ttctcctttc aggacgatgt
     3541 catcattcgg cgggtatcgg tcgtttgttt tagtttattg ttagcaacaa cctgcaagca
     3601 gcgtctcgcg gtagttttta atatgtgccg cgtatttcct atgatgctga ccccaaaaaa
     3661 taaaaacgct aaagtgaaaa aacaaaagaa gactccgcag gttcgtgaat caggacgaaa
     3721 ttcattgttt gtcgtgatgc tgataattat tgtgggatgg tgcctggtgt tcgccagtaa
     3781 taaagatatt gcattcatct caacaattat tgggttaatt gatcatttta tcggcccaat
     3841 aaccattgct ggcatcgttc tgctgatcat tgtggccaaa tttactggca atcttaagct
     3901 ggggtcagga gaaaaaagca acaggaaaac ggggaatcgt aatgctcatt ctcgctcttc
     3961 gtcgagaaat tctttccgcg gtggtggcgg ttcttcagga ggcggcggtt catccgtccg
     4021 ttggtgatcg taatgcgctt aatctttatt ctattcactt tgtggtgcct tcctgggctt
     4081 gcccagcaaa ttgctgtgcc ggaactgcgc caacaggtga ccgatattac tggtacgtta
     4141 agcacttctg aacaacaatc actgacacag caactgcagg atattacaca taaaacacgg
     4201 gcgcaggtcg ctgtattagt agtgccgtcc accggagacg attccattga acaatatgct
     4261 acaagggttt ttgatagctg gaaactggga gataagcaga gaaacgatgg tattttactt
     4321 ctggttgcct gggaggatca tgctgttcgt attgaggttg gctacgggct ggaaggggtg
     4381 gttaccgacc tgcaagcggc gaagattatc agggatatat tgatccctgc cttcaaaagt
     4441 gatgacctga tgggtggatt aacactggca agcgaaaata tcggcgcgct tctgttgaat
     4501 ggtgaattac cggaagacag gggggattat tacagtatca atccccctat tccattatcg
     4561 cttgctgtaa ttatattgct ggcggtactt tcatatttta ttgtttttac cgatccgtcg
     4621 aatttaccct ggatcactct cactggcgcc atttacggga tggtattcct ctatgttgcc
     4681 gagccaggac catggaccaa tttaatcgtt gcctgcggta tgttaacgcc ttttgcgatc
     4741 gtaccactgg ttattttttg gcttatcgtc aataaaaagc tacgcgccaa atacaaaaaa
     4801 ctcagtaaag atagagcttc aagaaaaggt tcttcttcat cctcttccgg tggcggctca
     4861 agtggcggag gctcaggcgg cggatttagc ggcgggggcg gttcttctgg cggtggcggc
     4921 gcgtccggtc gctggtaact caaccataaa aaacgccagc ccgaaggctg gcatttttaa
     4981 atcagataaa gtcagactta tgcctggcct ttgatctctt tacgaccgtt gtacggtgct
     5041 ttttcgccca gagcttcttc gatacgaatc agctggttgt atttagcaac gcggtcagaa
     5101 cggctcatag aaccagtttt gatctggcct gcagcagtac caacagccag gtcagcgatg
     5161 gtagcgtctt cagtttcgcc agaacggtga gagataactg cagtgtagcc agcgtctttc
     5221 gccatcttga ttgcagccag agtttcggtc agagaaccga tctggttgaa tttgatcagg
     5281 atggagttag cgataccttt ttcgatacct tctttcagga tcttggtgtt ggttacgaac
     5341 aggtcgtcac caaccagctg gattttgtcg cccagaactt tggtctggta tgcgaaacca
     5401 tcccagtcag attcgtccag accgtcttcg atggaaacga tcgggtactg tttggtcagt
     5461 tcttccagga agtgagtgaa ttcttcagag gtgaatgctt tgttgccttc gccagccaga
     5521 acgtatttac cgtctttgta gaattcagaa gctgcgcagt ccatcgccag agtgatgtct
     5581 ttgcccagtt cataaccagc agctttaaca gcttcagcga taacagccag agcttcagcg
     5641 ttggaaccca ggttcggcgc atagccacct tcgtcaccaa cagcagtgtt catgcctttc
     5701 gctttcagaa cttttgccag gtgatggaaa acttcagaac ccatgcggat ggcttctttc
     5761 acagttttcg cgccaaccgg ctgaatcatg aattcctgga tatcaacgtt gttgtcagcg
     5821 tgctcaccac cgttgatgat gttcatcatc ggaaccggca tggagtattt gcccggagtg
     5881 ccgttcagtt cagcgatgtg ctcgtacagc ggcatacctt tagcagctgc agcagctttg
     5941 gcgttagcca gagatacagc caggattgcg ttcgcgccga atttggattt gttttcggtg
     6001 ccgtccaggt cgatcatgat cttgtcaatg ccagcctgat ctttagcatc tttgccaatc
     6061 agcgcctgag cgatcgggcc gtttaccgca gcaacagctt tggttacgcc tttacccagg
     6121 aaacgggatt tgtcgccatc gcgcagttcc agagcttcac gggaaccagt agaagcacct
     6181 gacggagcag ctgccatacc gacgaaacca ccctccagat gtacttcggc ttcaacagtc
     6241 gggttaccac gggagtcgat gatttcacga ccgatgattt ttacgatttt ggacattagg
     6301 ttttcctcaa gtcactagtt aaactgaaac tccagacaaa caacgcgtac ccagggtacg
     6361 cgttgccgtt ctaacttttt tacttacttc gcctgacgtt tctggaactc gctggcggct
     6421 ttcacaaagc ctgcaaacag cgggtgacca tcacgtggag tagaagtaaa ctccggatgg
     6481 aactggcaag ccacgaacca cgggtgattc ggaacttcga tgatctcgac caactgatca
     6541 tccccggaac ggcccgcaac gcgcagacct gcatcttcaa tctgtttcaa cagcatgttg
     6601 ttgacttcgt aacggtgacg atgacgctca acaattgtcg gcgcattgta cagctggcga
     6661 accaggctat cgtcaaccaa ctggcactgc tgtgcgccga gacgcatggt accgccgaga
     6721 tcgctcttct cgctacgaac ttcaacgttg ccgttttcat cgcgccactc ggtaatcagc
     6781 gccacaaccg ggtacttaca gtctggcaca aattccgtag agttggcgtt ctccatgttg
     6841 gcaacatggc gagcgtaatc aattaacgcc acctgcatac ccaggcaaat gcccagataa
     6901 ggaatattgt tctcacgcgc aaaacgcgcg gtcgtaatca tgccttctac gccacgatag
     6961 ccgaaaccgc caggtacgag gattgcatcc agacctttaa ggatttcaac gccgcgcgtt
     7021 tcaacatctt gtgaatcgat cagtttgatg ttgacgctga cacgattctt cagcccaccg
     7081 tgtttcagtg cttcaatcac ggatttgtaa gcgtccggca gttcaatgta cttgccgacc
     7141 ataccaatgg tgacttcact taccggattc gcttcttcga agataacctg ttcccattcg
     7201 gacagatttg cttccgggca gtttaagctg aatcgtttac aaatataatc gtccagcccc
     7261 tgagatttca acaggcccgg aattttatag atggaatcga cgtctttcag agaaataacc
     7321 gctttttccg gaacattaca gaacaatgca atcttcgcac gttcgttcgc cggaacggcg
     7381 cgatctgaac gacaaatcag gatgtcaggc tggataccga tggagagcag ctcttttaca
     7441 gagtgctgag tcggtttggt tttgacttca ccagacgctg ccatgtacgg caccagcgtc
     7501 aggtgcataa acagagtgtg ctcacggcca atttcaacag ccatctggcg aatcgcttcg
     7561 aggaacggca gggattcgat atcacctact gtaccgccga tttcgaccag tactacgtca
     7621 tgaccttcgc caccttccag cacgcgctct ttgattgcgt tagtgatgtg cggaataacc
     7681 tgtacggttg cgccgaggta gtcaccgcgg cgttctttac gcagaacgtc agagtagata
     7741 cgacccgtgg tgaagttgtt gcggcggctc attttggtac ggatgaaacg ctcgtagtgc
     7801 cccaggtcca ggtcggtttc agcgccgtct tcagtaacga acacttcccc gtgttggatt
     7861 gggctcatag tacctggatc gacgttgatg tacggatcca gtttcatgat ggtcacattg
     7921 aggccacggg cttcaagaat ggctgcgagg gaggctgcgg caatgccttt acccagagag
     7981 gatacgaccc cgccggtcac aaaaatatag ttcgttgtca tgctgaacct gagaagttag
     8041 gttgaaaaga cgatggaata accaggacgg gaaagcagta tacccgaaca tgacctgtgc
     8101 cacaaacttt cattatccct cctcttcgcc agcgcactat tgaaatcagg agtgagaaaa
     8161 tagccccttt ggggtaaatg tttttgacgc aaatcaagcg cttgtcattt aaaaaatgac
     8221 acaaatggcg cttgaccgcg taattccctt agagatcaat ttcctgccgt tttacctgtt
     8281 gccagacttc ttccattgtt tcgaggtcaa cacctgtcat ttccagtcca cgcgcggcaa
     8341 caatacgctc cacttcgcga aaacgacgct cgaatttttc gttcgctttt tgcaatgcga
     8401 tttccgcttt tgtccctaaa tgacgagcca gattgaccgt ggcaaacagc aggtccccca
     8461 tttcctcctc cagtttagcc tggtcgacaa cagcctgccg cgcttcgtac atcacctcgt
     8521 cgatctcttc gtagacttta tcgactaccg gaccaagcgt cgtccaatcg aagccaacgt
     8581 tggcgcaacg tttctggatt ttttgcgcac gcattaaagc cggtaaacta cgaggaatat
     8641 cgtccagcgc cgaatgctgc gctttctgcg cgcgctcttc ggttttgatt tgctcccaac
     8701 gggcaagcac ttcactactg ttttcggcag aactgtcagc aaaaacatgc ggatggcgac
     8761 gctctaattt atcgctaatc gcagcgcaaa tatcattaaa gtcaaagcgc ccttcttcct
     8821 gagccatttg ggcgtaaaac accacctgga acagcagatc gccgagttca ccgcgcagat
     8881 cgtcaaaatc ttcacgggcg atggcgtcca gcacttcgta ggtttcttca agggtgtaag
     8941 gcgcaatggt ggcaaatgtc tgctctttat cccacgggca gccgttttcc gggtcgcgca
     9001 ggcgctgcat aatagtgagc aaacggtcga tttgattcat tagaatgtcc tgaaaattgc
     9061 gggtccgtca ggtggaaacc tgtgaccaga atagaagtga gttagtaaca ctacccaatc
     9121 agtacgttaa ttttggcttt aatgagttgt aattcctctg gggcaaccgt tcctttcttc
     9181 gttgctcctc ttgcccgcca ggcgatactt tttacctgat cagctaacgc tacgccatca
     9241 cgttcctgac cggataaaac aacttcgaac ggatatcctt ttgattgcgt tgtacaagga
     9301 acacacagac acatacctgt tttgttgttg tacatgaacg gactcaggac aacagccgga
     9361 cgatgtccgg cttgctcgct accttttgtc gggtcaaaat caacccaaat cagatcgccc
     9421 atatcgggta cgtatcggct taccattacc agacttcctt atctttcggc tctccccagt
     9481 cgatattctc gtggaggttt tccggcgtga tgtcgttgac cagttcagca agcgtaaata
     9541 cgggctcttt acgcactggc tcaataatta atttgccatc caccaggtca atcttcactt
     9601 catcatcaat attcagattg agcgcctgca ttaacgtagc cgggatccgc accgccggtg
     9661 aatttcccca acgctttacg ctactgtgga tcataaccct ttcctcaaac cactatcata
     9721 tgtagataca gtatatatca atctacattg tagatacgag caaatttcgg cctaactccc
     9781 gtgcaaccga cgcgcgtcga taacatccgg cacctggttg agtttaccca gcacgcgccc
     9841 cagcacttgc aggttgtaaa tctcaatggt catgtcgatg gtcgccagtt gctgtttggt
     9901 gtcgctacgg ctggcaacgc caagcacgtt caccttctcg ttggcgagaa tggtcgtgat
     9961 atcacgtaac aacccactac gatcattagc caccacgcgg accaccagcg aatatccggc
    10021 ggagtagctc tcaccccata ccgcgtcaac aatgcgttct ggcgcatggg agcgcagttc
    10081 cgccagttgt tcgcaatcgg cgcggtgtac tgaaataccg cgtccctggg taatgaagcc
    10141 gacaatctca tctccaggaa tcggctggca gcagcgcgcg atgtggtgca tcaggttacc
    10201 aacaccttca accactacac gaccgttatc tttactgcgg ttttgcggcg tgtagctttt
    10261 ttgctgaagc tgtttcagcg cggcggcgtc ctgctcttcg gcactcggct tattaaattg
    10321 cgattgcagg aagttcacca tctgattgag acggatatcc ccgccaccaa tcgccgccag
    10381 caactcgtcg acatcattga agttgtaacg cggcagcaga tgtttttctg cttctttcag
    10441 gctgatcccc agatgttcca gctcgtcgtc aaggatttgc cgcccagcca gaatgttttt
    10501 gtcacggtcc tgtttacgga accaggcgtg aattttcgaa cgcccacggc tggttgtgac
    10561 gtaaccgagg tttgggttta accagtcacg gctggggttc ggctgtttct gggtgataat
    10621 ttcaatctgg tcgcccattt gcagctggta ggtgaacggc acaatgcgcc cgccaatttt
    10681 tgccccgata cagcggtgtc cgacatcact gtggatgtgg taagcgaagt ccagcggcgt
    10741 tgatcccgca ggcaaatcaa cgacatcacc tttaggcgta aagacgtaca cccggtcgtc
    10801 aaagacctgg ctgcgtactt cgtcgagcat ttcgccggaa tcagccatct cttcctgcca
    10861 cgcaatcagt ttacgcagcc aggcaatccg gtcttcatgt cccgaacgtg cgccgccagc
    10921 agccgcgccc tctttatatt tccagtgcgc agcaacaccc aactctgcat cttcatgcat
    10981 ctgtttggtg cggatttgga tctcaacggt ttttccaccc ggccccagaa ccacggtatg
    11041 aatagactga taaccgtttg gtttcgggtt agcgacgtaa tcgtcaaact catccggcag
    11101 gtggcgatag tgagtgtgca ctatccccag tgcggcataa caatcctgta aacgctcggc
    11161 gacaatacgt accgcacgca catcaaacag ctcatcgaag gcgaggttct ttttctgcat
    11221 tttgcgccag atgctgtaga tgtgtttcgg acgaccatac acttcagctt taacgccttc
    11281 agctttcatc tcagcgcgca gatgaccgac aaactcttcg atatagtgtt cgcggtcgag
    11341 acggcgttca tgcaacagtt ttgcgatgcg tttgtattcg gtcgggtgga gataacggaa
    11401 gcagtaatct tccagctccc atttcagttg cccaatccca agacggtttg ccaacggcgc
    11461 gtagatattg gtgcactctt ttgccgccag tacgcgttca tcttccggcg catcttttac
    11521 ttcacgcaga tgagcaatac gctccgccag tttgatgacc acgcagcgaa aatcatcgac
    11581 catcgccaat aacatccggc gaacgttatc gacctgttcg gaggaaacag aatcagtgtg
    11641 cgtcgctttc agctggcgga tcgccgccat atcacgcacg ccgtgaataa ggttaacgac
    11701 cgacttaccg acgctctcac gcagcacatc ttcgctgact acgttggcat cagccagagg
    11761 gaacagcagc gccgcccgca gcgtgtcaat gtccatactt aatgtcgaga ggatctccac
    11821 catctcaaca ccacgccaca ataacagact ggcatccgga tgcccctgcg tctgttgcag
    11881 acaatacgcc caggtttcgg ctaagcactc acacgacttc tggctggtaa tacccagact
    11941 tgcgatccat ttttccggat caaattcacc agccttattg atatgtgcac ttcttaccgc
    12001 aaccatcgtc ctctccttta gggaccagac ctgccgaaat cggcaaatcg caactattta
    12061 acgcgcgaga aaagtaccat cgattccaga tgtcccgtgt gtgggaacat atccagcatc
    12121 gccagtcgcg caatggtata tcctgctttt aataacgctt cgctatcccg agccagcgtt
    12181 gcagggttac aggatacata aactatacga ataggttcca gttttataat ttgctgcata
    12241 acacctgcgg cacctgctcg cgccgggtcc agcaacactt tatcgaagcc gtttttcgcc
    12301 cacggctgct ttgtgacatc ttcttcaaga ttttcgtgat aaaacgtcac attctgtaag
    12361 cagttaagac gcgcattctg ctggcctttt tccaccagcg ccggaacacc ttctacaccc
    12421 accacactgg cagcttgtgt cgccaatggt agtgtaaagt tgcccatacc gcagaacaga
    12481 tccagtacgc gatcttcagg ttccacctcc agccattcca acgcacgcgc taccattttt
    12541 tggttcacac ccgcattaac ctgaataaaa tcgcgcgggc taaaagttaa gcgcaacccg
    12601 tttgagtcat accagggcat ctcaccagag acggtttcga gtatctcact atcgggggcg
    12661 agatacagat ccaggccttc agaatgcgaa aagcgttcca gtttttcgcg atctgccgaa
    12721 cttagcggtg cggtatggcg caaaatcatc agcgtgccgc tggttgcctg taccagttca
    12781 acatgaccaa gatggcgcat agcttgtaag ctgcccaggc atgccctgac tttgggcagc
    12841 aatgcttcaa gttggggcac taaaatggga cattgtttaa cgtcgacaat gtcactggag
    12901 cccgctttgc gaaaccccat ctgaagttgc tgtgttttcg gtaagtagtt cagacttaaa
    12961 cgcgcgcgac ggcgatagcc ccagggaaca tcggcgatca cttcagagac ttcgtgtttc
    13021 attaatcggg cgagtgccgc acttttgctt cgctgctgta aatccacgct ggcgtgttgt
    13081 tgctgacagc cgccgcatac gccaaaatga ggacagcgtg gcgtttcgcg ttccgggcta
    13141 tcgcttaacc ggcgtacgac tttagcgcgg gcatactgtt ttttatcctc agtaacagta
    13201 acttccgcgt tttcctgcgg caataatccg gggataaata gcgttttgcc gttatgtcgc
    13261 gccacgccct gaccaaaaga gtcgaggtcg ttgactgaaa cggttatgat ctgacgcgtc
    13321 gtcgtgcgtc gttttgcaga gtagaattgc gccattggcg agactttctc aatttaacag
    13381 tgtgacctta attgtcccat aacggaactc catgaccaac tacagcctgc gcgcacgcat
    13441 gatgattctg atcctggcac cgaccgtcct tattggttta ttgctgagta tctttttcgt
    13501 cgtgcatcgc tataacgact tgcagcgtca actggaagat gccggtgcca gcattattga
    13561 gccgcttgca gtttctactg aatatggcat gagcctgcaa aatcgcgaat ctatcggtca
    13621 gttaataagc gtactgcatc gtcgccattc cgatattgtt cgcgcgattt cggtttatga
    13681 tgaaaataac cgactctttg tcacctccaa ttttcatctt gatccctcat caatgcagct
    13741 cggcagcaac gtgccgtttc ctcgccagct cactgtcact cgtgacggcg atattatgat
    13801 cctccgcacg ccgattattt ctgagagtta ctcccccgac gaatcgccca gtagcgatgc
    13861 caaaaatagt caaaatatgt tgggatatat tgcgcttgag ctggatctta aatcggttcg
    13921 cttgcagcaa tataaagaga tctttatttc cagcgtgatg atgctgtttt gtatcggtat
    13981 tgcgcttatt tttggctggc gcttaatgcg cgatgtaacc ggtccgattc gcaacatggt
    14041 gaatactgtc gaccgcatcc gtcgcgggca actcgacagc cgagtggaag gatttatgct
    14101 cggcgagctg gatatgctga aaaacggtat caactcgatg gcaatgtcgc tggctgctta
    14161 tcacgaagag atgcagcaca atatcgacca ggcgacgtcc gatctgcgtg aaacgctgga
    14221 gcagatggaa attcagaacg ttgagttgga tctggcgaaa aagcgcgccc aggaagcggc
    14281 gcgtattaaa tccgagtttc tggcaaatat gtcacacgag ctgcgtacac cactgaatgg
    14341 tgttattggt tttacccgcc tgacgctgaa aacagaatta acaccaacgc agcgcgatca
    14401 cctgaatacg attgaacgtt cggcaaataa tttgctggca attattaatg atgttctcga
    14461 cttctcgaaa ctggaagcag gtaagctgat tctggaaagt attccattcc cactacgcag
    14521 cacgctggat gaagtcgtta ctctgctggc acattcttct cacgataaag ggttggaact
    14581 gacgctcaat attaaaagcg acgtgcctga taacgtgatc ggcgacccac tgcgattaca
    14641 gcaaatcatc actaacctgg tggggaatgc aattaaattc accgagaatg gcaacattga
    14701 tattctggta gaaaaacgtg cgctgagtaa taccaaagtg cagattgaag tgcagattcg
    14761 ggataccggc attggtattc ctgaacgcga tcaatcgcgc ttattccagg ccttccgaca
    14821 ggctgatgcc agtatttccc gccgtcatgg tggcaccggg ctggggctgg tgattacaca
    14881 aaaactggtt aatgaaatgg gcggcgatat ttcgttccat agccagccga atcgcggttc
    14941 aactttctgg ttccacatta atctcgatct gaacccgaac attattattg aagggccatc
    15001 cacccagtgc ctcgccggta aacgcctggc ctatgtcgaa ccaaactccg cagcagcgca
    15061 atgcacgctg gatattttaa gtgaaacgcc gctggaagtg gtttatagcc caacgttctc
    15121 cgcgctgcct cccgcgcatt acgacatgat gttgttaggc atcgcggtga ccttccgcga
    15181 gccgctaaca atgcaacatg agcgattagc gaaagcggta tcgatgaccg atttcctgat
    15241 gctggcactt ccttgccatg cacaagtcaa tgctgaaaaa ctcaagcaag atggtatcgg
    15301 cgcgtgtctg ctgaaaccat taacacctac gcgcctgttg cctgccctga cggaattttg
    15361 tcatcacaaa caaaacacgc ttttgcctgt aaccgatgaa agtaagctgg caatgacagt
    15421 catggcggtt gatgacaacc ccgctaacct gaaacttatc ggcgcattgc tggaagatat
    15481 ggtgcaacat gtggaacttt gcgatagcgg gcatcaggcg gttgaacggg cgaaacagat
    15541 gccgttcgat ttgatcttaa tggatattca aatgcctgac atggatggca ttcgggcctg
    15601 cgagctcatc caccagctcc cccatcagca acaaacgccg gttatcgcgg taacggcgca
    15661 tgcaatggcc gggcaaaaag agaagctgct tggcgcaggg atgagcgatt atctggcgaa
    15721 accgattgaa gaagagcgat tgcataattt gttgttgcgc tacaagcctg gctgcggtat
    15781 ttcgtctcgc gtcgtgacgc ccgaagtcaa cgaaattgtg gtgaacccga atgcgaccct
    15841 cgactggcaa ctggcactac gccaggcagc aggaaaaacc gatttagcgc gcgatatgct
    15901 gcaaatgtta ctcgatttcc tgcctgaagt tcgcaacaaa gttgaggaac agctggttgg
    15961 agaaaacccg gaaggcctgg ttgatttgat tcataaactg catggcagtt gcggctatag
    16021 cggtgtgccg cgtatgaaga atctctgcca acttatcgaa caacagctac gtagcggtac
    16081 taaagaagaa gatttggaac cggagctgct ggaactgttg gacgagatgg ataatgtcgc
    16141 gcgcgaagcc agcaaaattc tcgggtaacg gacgtcttgt cgagtagcaa tcggcttttt
    16201 atgacgctgt tttcagattg gtgacaaagt gcctgtgttt atgccggatg cggcgttaac
    16261 gccttatccg gcctacaaaa ccaattaaat tcaataaatt aaaaagcatg taggcctgat
    16321 aagcgtagcg catcaggcaa ttttgtgttt ggcatcagtc tcaaaccggc tccagataga
    16381 gccggttttg gttttttgtc ttaacgcacc atgcacgggc gcttgttatc gaacgtccag
    16441 ccaggaatca gatactgcat tcccatcgca tcgtcacgcg cgccaagccc gtgtttctga
    16501 tacagctcat gggctttcat cacttgatcc atatcgattt ctacacccag ccccggtttt
    16561 tctggcacct gtaccagccc gcctttgatc tcaaacggtt ctttggtcag gcgctgatta
    16621 ccttcctgcc agatccagtg cgtatcaata gcagtaattt tacccggtgc agcggcggca
    16681 acatgggtaa acatcgccag ggaaatatcg aagtggttgt tagagtgtga accccaggtc
    16741 aggccaaatt catggcacat ttgcgccaca cgtaccgaac cttgcattgt ccagaaatgc
    16801 ggatccgcca gcgggatatc aacggattgt aacgagagcg tatggcccat ctgccgccag
    16861 tcggtggcga tcatattggt tgcagtcagc aggccagtcg cgcgacggaa ctctgccatc
    16921 acttcacgcc cggagaaacc ttgctccgca ccacacggat cttctgcata agccagcgaa
    16981 cctttcaggt atttgccgat tttaatcgct tcgttcagcg accaggcacc gttaggatcg
    17041 agcgtaatac gcgcctgcgg gaagcgttgc gccagtgcca caatagactc ggcctcttct
    17101 tccccggcca gtacaccgcc tttcagtttg aaatcgttga agccatattt ttcatacgcc
    17161 gcttccgcca ggcgcaccac cgcatccggc gtcatcgctt cttcatgacg caggcgatac
    17221 cagtcgcatg agtcatccgg ctggctttga tacggcagag gtgtggcttt gcgattaccg
    17281 acgaagaaca gataaccgag catttcgact tcgctacgct gttgaccatc gcccagcagg
    17341 gatgccacgt taacccccag atgctgcccc agcagatcca gcattgccgc ttctatcccg
    17401 gtaactacat gaatagtggt acgcaaatca aatgtctgca aaccgcgccc gccggcatca
    17461 cgatcggcaa aagtattacg caccagcgtc agaacgtttt tgtattcacc cagcgtttta
    17521 cccactacca gcggaattgc atcttccagc gttttacgga ttttctcgcc gccgggaatt
    17581 tcccctacgc cagtgtgacc agaattatct ttgataatca caatattacg cgtgaagaac
    17641 ggtgcgtgtg caccactcag attcatcagc atactgtcat ggcccgccac cgggatgacc
    17701 tgcatttcag taacaacagg cgtcgtaaat tgagaactca tatttatgtc ctttttcaga
    17761 atcaatgacg gccgaaaacg ggacgtttac ggtcaaaggt ccagccgggg atcaggtact
    17821 gcatcgggcc tgcgtcgtta cgcgcaccgc caggcagacg tttataggcc tcatgtgcct
    17881 tttgtacctg ttcccagtcc agttccacgc ccagaccggg cgcatcagga acggcaattt
    17941 ttccgttttt aatctccagc ggattttggg tcaggcgaca atcgccctcc tgccaaatcc
    18001 agtgggtatc gatagcggta ggattacccg gtgccgccgc gcccacatgg gtaaacatcg
    18061 ccagagagat atcgaaatgg ttgttggaat ggcagcccca ggtcagcccc cagtcgtcgc
    18121 aaagctgcgc cactcggact gcaccggaaa gcgtccagaa gtgcggatcg gcaagtggaa
    18181 tatctaccgc attgagcatc accgcatgac ccatttcgcg ccagttggtg gcgatcatat
    18241 tagtcgcgac gggcaagccg gtcgcccgcc gaaactccgc catcacttca cgtccggaga
    18301 agccctgttc tgcgccgcat ggatcttcgg cataggtaag aacatcattc agccctttgc
    18361 acagagaaat ggcttcatca agcagccatg caccgttggg atcaacggta atccgcgcat
    18421 ccgggaagcg tttcttcaat gcacgaacag tgtcgatttc ttgctcgcca ggtaacacgc
    18481 cgcccttaag tttgaaatct ttaaagccgt agcgatcctg tgaggcttcc gccagacgca
    18541 caacggcttc gctgttcatc gctttctgat ggcgcaactg ataccactca tggttgcccg
    18601 gcgtattttc cagataagga agatcggttt tggtccgatc accgatataa aacagataac
    18661 cgaggacggt aatagcatcg cgttgcttgc ctggccctaa cagttcgcag accggaacat
    18721 tcagcgcctt acctagcagg tcaagcaagg cggcttccag cgccgccacg gcgttaacgc
    18781 gcaattcaaa ggtccaggcc cctttaccga aggtatcaaa atcggctgct tgattacctt
    18841 tatgcacctg ctgaaccact ttattcaggc gcgcaacttc ctagcccagc accatcggaa
    18901 tagcatcgac caatgtctga taaatcacct ctccgcctgg cgcttcacca atgccggtat
    18961 gcccggcgtt atcggtgagt accacaatat tgcgagtgaa atatgcgtta tgcgccccac
    19021 caatattaag caacatgctg tcgtacccgg cgaccggaat gactttcata tcagtaataa
    19081 caggactgga ttgtgtcgtc atcttattgc cccacgacag gtttcagctc gatacgcttg
    19141 atatcgccca ccagcaccag gtagctcagt accgcgatta aggcatgaac accaacataa
    19201 atcagcgccc cattaaacga gccagtcgtg ccaacgatat aaccaattgc gattggcgtg
    19261 acgatgccag aaatgttacc gaacatgttg aacaggccac cggaaagacc gctgatctct
    19321 tttggcgcgg tatctgccat tactgcccaa cccagcgccc caatgccctt accgaagaag
    19381 gccagcgcca taaagccgat gatcatccac tcaacgttga cgtagttgca gaacaccatc
    19441 accatcgaca gcaacatgcc cattacgatc ggtgttttac gcgcaatgtt cagcgatccc
    19501 gtgcggcgca tcagccagtc ggaaataatc ccacccagca caccaccgat aaaaccacaa
    19561 accgccggaa cggaagccac aaagcccgct ttcagaatcg acatcccacg cgcctgcacc
    19621 agataaaccg gaaaccaggt aatgaaaaag taagtcaggg cgttgataca gtactgaccg
    19681 atataaacgc cgatcatcat ccgtgacccg agcagttgtt tgatctgccc ccacttcacg
    19741 ctgaacggca ctttaacttt ggtgttttgc tgatccatat tgatcagcgc gccacccgcg
    19801 gcgatgtact ccagctcttt ctgatttacc cccggatgtt gatttggctc gtggatgact
    19861 ttcaaccaga taaagctgat ggcaatgccc agaccgccca taaagaagaa gacgtgtgac
    19921 cagcccactt catgcgtcag ccagcccata atcggcgcga agatcaccgt tgcgaagtat
    19981 tgagcggagt taaaaatcga caccgccgtt cccctttcct gcgccggaaa ccaggccgca
    20041 acaatgcgac tgttgccggg gaaagatggc gcttcagcaa gcccgaccag gaagcgcaga
    20101 gtaaacaggg caacgataat gccgaatcca ctaaagatat cgacgaagcc ttgcagcaag
    20161 gtaaacatcg accagataaa gatcgaccag aagtagacgc gttttgaacc aaaacgatcc
    20221 agcaaccaac caccagggat ctgcccgata acataagccc atgagaaggc agagaacaca
    20281 tagcccattc ccacgggatc aaggccgata tctttggcca tttccgaacc ggcaatagaa
    20341 agcgtagcgc ggtcgccgta gttgaaggat gtgacgataa acaacatcac cactatccag
    20401 taacgagcat tcgtgcgttt ttccacactg ctcgcagcct gacttaaaga actcattgtt
    20461 gcactcctga aaattcgcgt tagccacgct cactctggac tgcgacatcg ccaggaaatc
    20521 agaggtgacg tagggtgttt tttgccgttt ttataggtcg ttcgccgaat acggcgcgtg
    20581 tttatatctg gcaatagcag tataaaaagc gcgccatagc ggctcaccgt gcaacaacac
    20641 aacattaatg cgttcaatga ggcccgattt tggcattagc cctggacggt ggaatccact
    20701 tcacggaaat gaaaacaaga acaagaaagg aagggttaaa acgaagaaat aaagaagagt
    20761 atgaaatgga tcgcttgact ccaggcaaac gccagtaaaa atccgcgcta tgaagcagtt
    20821 tttactggca tttgcctgaa aagattcgat tcagcaccgc taaaacgaca tttaccgctc
    20881 gctgaacata tcaggacaac agcgtgcccc actgttcgac ccacggattt gattccgttt
    20941 ccggttccgg gttctcactg gcgtcaatca acagcatttc gccaacccgc tgagcgctct
    21001 gttcctgcag caaggcatcg aactgtttgc cgccattgca gaaattcaca taactactgt
    21061 cgccgagcgc aatcacgcca taacgcagat tcggctggaa gcccagacta tctttgattc
    21121 cctgaaagag cggcacaatg ctatcaggaa ggtcgccctg cccggtcgtg gacgtaacca
    21181 ccagaacata cttatcctga tagggcagcc agtcgcttaa ttcaggatct tcaaataccg
    21241 ttgctttgtg gccctgtgcg gtcagaattg cttctgcttc ttccgccact aacagcgaat
    21301 ttccgtacat ggtgccgaca aaaataccaa tttccgccat accgttttct ccctggatta
    21361 ggaacttctc tctccatcct gacccgatgg cgcactgaac tcaacccttt cattttcagg
    21421 aagcagaccg cgccagccaa attgtgataa cgcctgcatc caggtgtcgt ccaggcccgc
    21481 gtgaatagtc agcggctcac cagtaaaagg atgcgtcaat gacaactgac tggcatgcag
    21541 cattaaccgc tgaagaccaa aatgctcagc accgctgcga ttctggcgta aatcgccatg
    21601 tttgctatcg ccaataatcg gatgacgcaa atgggcaaga tgtcggcgaa gctgatgttt
    21661 gcgtccggtt ttcggctcca gttccaccag gccgtagcgc gtggtcgggt aacgtccggt
    21721 cgctaccggc atttctacgg tcgccagacc gcgataatgc gtcactgctg gctgcgggcc
    21781 tttatcttcg cgggcaaatt tatcagcgat tttgtccagt tcttccacca gtggataatc
    21841 cagcaccgct tcttccatca accagccgcg cacaatcgca tggtaacgtt tctggatttg
    21901 gtgctgttca aactgttgtg ccagcagccg tccggcctcg ctggataatc ccatcaacaa
    21961 cacaccagaa gtgggtcggt ccagacgatg agcagtaaaa acatgctggc ctatctggtc
    22021 acgcacggtt tgcatgacca ctactttctc gtcgcgatcc agccagctac ggtgaaccag
    22081 ccagccggag ggtttattta ccgcaaccag ccattcatcc tgatagagta tttccagcat
    22141 tagctcgcat catccgcaaa aagagcatcc agtttttcca actcagccag aataagcgcg
    22201 cgttgcggat ggtccgtcgc cagcgccatt tcataatagg gtgcaacggc aaaagcgccc
    22261 ggtaacggct ggttgttatt taacaaatcg tgcattcgcg ggatcagcac ccactgcaac
    22321 cactccagtg gttccatggt gtccataaag aacggttggg tactattaaa ttgatgcggc
    22381 tggggttcat cgtttcgcca gtgctgatgt tcacgcagta acgcttcaag cgcctgcaac
    22441 tggagacgaa cgcggtcatg agtggtcata aagcaacctc aataagaaaa actgccgcga
    22501 aggatagcag gaataaaaaa agggagcact gtattcacag tgctcccggt tcgtttcgca
    22561 gcattccagc tacttttgtt gctccctgct catccttgac aacttttcct ctggccatgc
    22621 ggccaatcgt tcatcctgaa ctattgcttc ctgctcacac caccccgatg tgatacttca
    22681 tcctgaagtg tcccaggcct tcctgaccca ccgaatcatc ctgaccggtt ctcattctcc
    22741 atcctggagg tgtcctttaa cgcgtcctgc gtcatcctct tcgcttcatc cagaagcctt
    22801 tccctgaaac accatcctgg tgtgtccttc agaagtgtca tcatcctgat gttcacttcg
    22861 ttgtctgact ccctgtcgac gaagatagaa tcgtcttttt cgcgaactct tacaaggcgc
    22921 ttacagacaa tgccttaagg aaaatttcat atgaaagtta ggaattaaca agcaacatgt
    22981 tgtttttaaa tgaaaataac gattcttatc tttttaagta ttcgcttatt tcctggcgat
    23041 ctcctacaga gtctgtaaga gatctctcac aaccagatgg ggtatttgga ttacagaaga
    23101 ggcttaagtt gattaaggaa ttccgcaaga tttggggcta aatgcgtccg tttacgtgtt
    23161 cccagcgtct ctttacacac ttcaccggag agattacaca ccgagatgac ctctaactct
    23221 tcttccagcg tcgcgataaa tagcgtgggt ggcagtttta gccttttctg agtcaccaga
    23281 tgaccaatca gattttcctg cacccgacgg aagtcgtctt cactccaggt ttgcagcaat
    23341 gtcagtttga tatcgccaaa ctgcgcgtgc atatccccgg caaactgagt cgtataaaag
    23401 gtatgaattg tgggttgtat cacaatatca aaagcgcgtt caaccgcgtt tacattttgt
    23461 tccccggtaa acggctgggg ttgccagtat acggcatctt cagtagttga aataatgcac
    23521 ggtgaaggaa cgccgtacag ttcctcactt aacggccagc ttttatgctc ttcatgccac
    23581 gcatcgcagt agcgtgcagt aaaatctttc agggcttgtg cggtcaaatc gtccactaat
    23641 ttctctcttc acttaaacca gatacacttg tcgtttagtt tatctggttt atgacggtga
    23701 aacatgtctt cttatgcaaa ccatcaggca cttgcgggcc tgactcttgg aaaatcaacc
    23761 gattaccggg atacctatga cgccagccta ctgcaaggcg ttccacgcag cctgaatcgc
    23821 gacccgctgg gtctgaaagc ggataacctg ccttttcaag gtacggatat ctggacgctg
    23881 tatgaacttt cctggctgaa tgcgaaaggt ttgccgcagg tcgctgtcgg tcatgttgaa
    23941 cttgattaca ccagcgtaaa tctgattgag tcgaagagtt ttaagctcta tctcaacagt
    24001 tttaaccaga cgcgttttaa taactgggat gaggtgcgcc agacgctgga gcgcgactta
    24061 agcacttgcg ctcagggtga ggttagcgtg gcgttatatc gtcttgatga actggaaggc
    24121 cagccgatag gtcatttcaa tggcacttgc attgatgacc aggatatcac tatcgataac
    24181 tatgaattca ctactgacta tctggagaat gccaccagtg gtgaaaaagt agtggaagag
    24241 acgctcgtca gccacctgct gaaatcaaac tgcctgatca cccatcaacc agattggggt
    24301 tcgatccaaa ttcagtatcg tggacgccaa attgacagag aaaaactgct gcgttacctg
    24361 gtctcattcc gtcatcacaa cgagttccac gaacagtgcg tggaacgcat ctttaatgac
    24421 ctgttacgct tctgccagcc agagaaattg agcgtttacg cacgttatac ccgtcgtggc
    24481 ggtctggaca ttaacccgtg gcgcagtaat agcgattttg tcccatcgac cacaagactg
    24541 gttcggcaat aaattttttc tcaattttgc gtgctggatt cacgcagaag gttgtgaaag
    24601 gtcatcaggc agggctattg taatcaaagg gaatgacgat attcgtccca taaggagttt
    24661 ttcttgatta cacatattag cccgcttggc tccatggata tgttgtcgca gctggaagtg
    24721 gatatgctta aacgcaccgc cagcagcgac ctctatcaac tgtttcgcaa ctgttcactt
    24781 gccgtactga actccggtag tttgaccgat aacagcaaag aattgctgtc tcgttttgaa
    24841 aatttcgata ttaacgtctt gcgccgtgaa cgcggcgtaa agctggaact gattaatccc
    24901 ccggaagagg cttttgtcga tgggcgaatt attcgcgctt tgcaggccaa cttgttcgcg
    24961 gttctgcgag acattctctt cgtttacggg caaatccata ataccgttcg ttttcccaac
    25021 ctgaatctcg acaactccgt ccacatcact aacctggtct tttccatctt gcgtaacgct
    25081 cgcgcgctgc atgtgggtga agcgccaaat atggtggtct gctggggcgg tcactcaatt
    25141 aacgaaaatg agtatttgta tgcccgtcgc gtcggaaacc agctgggcct gcgtgagctg
    25201 aatatctgca ccggctgtgg tccgggagcg atggaagcgc cgatgaaagg tgctgcggtc
    25261 ggacacgcgc agcagcgtta caaagacagt cgttttattg gtatgacaga gccgtcgatt
    25321 atcgccgctg aaccgcctaa cccgctggtc aacgaattga tcatcatgcc ggatatcgaa
    25381 aaacgtctgg aagcgtttgt ccgtatcgct cacggcatca ttatcttccc tggcggtgtg
    25441 ggtacggcag aagagttgct gtatttgctg ggaattttaa tgaacccggc caacaaagat
    25501 caggttttac cattgatcct caccggcccg aaagagagcg ccgactactt ccgcgtactg
    25561 gacgagtttg tcgtacatac gctgggcgaa aacgcgcgcc gccattaccg cataatcatt
    25621 gatgacgccg ctgaagtcgc ccgtcagatg aaaaaatcga tgccgctggt gaaagaaaat
    25681 cgccgtgata caggcgatgc ctacagcttt aactggtcaa tgcgcattgc gccagatttg
    25741 caaatgccat ttgagccgtc tcacgagaat atggctaatc tgaagcttta cccggatcaa
    25801 cctgttgaag tgctggctgc cgatctgcgc cgtgcgttct ccggtattgt ggcgggtaac
    25861 gtaaaagaag tcggtattcg cgccattgaa gagtttggtc cttacaaaat caacggcgat
    25921 aaagagatta tgcgtcgtat ggacgacctg ctacagggtt ttgttgccca gcatcgtatg
    25981 aagttgccag gctcagccta catcccttgc tacgaaatct gcacgtaatc tcccctcccc
    26041 cggtgacgtt tgttgccggg ttttctcgtt tttggtcact tactcatcaa ctcatttcat
    26101 ttgttatatg aatgtttctt accaccctca cgcgacaaat atc
//
LOCUS       DAZKIP010000058        26068 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZKIP010000058 DAZKIP010000000
VERSION     DAZKIP010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.058"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            198..392
                     /gene="bfd"
                     /locus_tag="ACHOUK_002627"
     CDS             198..392
                     /gene="bfd"
                     /locus_tag="ACHOUK_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFP9898044.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            465..941
                     /gene="bfr"
                     /locus_tag="ACHOUK_002628"
     CDS             465..941
                     /gene="bfr"
                     /locus_tag="ACHOUK_002628"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFP9898045.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVND"
     gene            complement(938..1405)
                     /locus_tag="ACHOUK_002629"
     CDS             complement(938..1405)
                     /locus_tag="ACHOUK_002629"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFP9898046.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            1784..2095
                     /gene="rpsJ"
                     /locus_tag="ACHOUK_002630"
     CDS             1784..2095
                     /gene="rpsJ"
                     /locus_tag="ACHOUK_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFP9898047.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2128..2757
                     /gene="rplC"
                     /locus_tag="ACHOUK_002631"
     CDS             2128..2757
                     /gene="rplC"
                     /locus_tag="ACHOUK_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFP9898048.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2768..3373
                     /gene="rplD"
                     /locus_tag="ACHOUK_002632"
     CDS             2768..3373
                     /gene="rplD"
                     /locus_tag="ACHOUK_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFP9898049.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3370..3672
                     /gene="rplW"
                     /locus_tag="ACHOUK_002633"
     CDS             3370..3672
                     /gene="rplW"
                     /locus_tag="ACHOUK_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFP9898050.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3690..4511
                     /gene="rplB"
                     /locus_tag="ACHOUK_002634"
     CDS             3690..4511
                     /gene="rplB"
                     /locus_tag="ACHOUK_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFP9898051.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4528..4806
                     /gene="rpsS"
                     /locus_tag="ACHOUK_002635"
     CDS             4528..4806
                     /gene="rpsS"
                     /locus_tag="ACHOUK_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFP9898052.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4821..5153
                     /gene="rplV"
                     /locus_tag="ACHOUK_002636"
     CDS             4821..5153
                     /gene="rplV"
                     /locus_tag="ACHOUK_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFP9898053.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5171..5872
                     /gene="rpsC"
                     /locus_tag="ACHOUK_002637"
     CDS             5171..5872
                     /gene="rpsC"
                     /locus_tag="ACHOUK_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFP9898054.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5885..6295
                     /gene="rplP"
                     /locus_tag="ACHOUK_002638"
     CDS             5885..6295
                     /gene="rplP"
                     /locus_tag="ACHOUK_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFP9898055.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6295..6486
                     /gene="rpmC"
                     /locus_tag="ACHOUK_002639"
     CDS             6295..6486
                     /gene="rpmC"
                     /locus_tag="ACHOUK_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFP9898056.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6486..6740
                     /gene="rpsQ"
                     /locus_tag="ACHOUK_002640"
     CDS             6486..6740
                     /gene="rpsQ"
                     /locus_tag="ACHOUK_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFP9898057.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6905..7276
                     /gene="rplN"
                     /locus_tag="ACHOUK_002641"
     CDS             6905..7276
                     /gene="rplN"
                     /locus_tag="ACHOUK_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFP9898058.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7287..7601
                     /gene="rplX"
                     /locus_tag="ACHOUK_002642"
     CDS             7287..7601
                     /gene="rplX"
                     /locus_tag="ACHOUK_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFP9898059.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7616..8155
                     /gene="rplE"
                     /locus_tag="ACHOUK_002643"
     CDS             7616..8155
                     /gene="rplE"
                     /locus_tag="ACHOUK_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFP9898060.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8170..8475
                     /gene="rpsN"
                     /locus_tag="ACHOUK_002644"
     CDS             8170..8475
                     /gene="rpsN"
                     /locus_tag="ACHOUK_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFP9898061.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8509..8901
                     /gene="rpsH"
                     /locus_tag="ACHOUK_002645"
     CDS             8509..8901
                     /gene="rpsH"
                     /locus_tag="ACHOUK_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFP9898062.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8914..9447
                     /gene="rplF"
                     /locus_tag="ACHOUK_002646"
     CDS             8914..9447
                     /gene="rplF"
                     /locus_tag="ACHOUK_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFP9898063.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9457..9810
                     /gene="rplR"
                     /locus_tag="ACHOUK_002647"
     CDS             9457..9810
                     /gene="rplR"
                     /locus_tag="ACHOUK_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFP9898064.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9825..10328
                     /gene="rpsE"
                     /locus_tag="ACHOUK_002648"
     CDS             9825..10328
                     /gene="rpsE"
                     /locus_tag="ACHOUK_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFP9898065.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10332..10511
                     /gene="rpmD"
                     /locus_tag="ACHOUK_002649"
     CDS             10332..10511
                     /gene="rpmD"
                     /locus_tag="ACHOUK_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFP9898066.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10515..10949
                     /gene="rplO"
                     /locus_tag="ACHOUK_002650"
     CDS             10515..10949
                     /gene="rplO"
                     /locus_tag="ACHOUK_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFP9898067.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10957..12288
                     /gene="secY"
                     /locus_tag="ACHOUK_002651"
     CDS             10957..12288
                     /gene="secY"
                     /locus_tag="ACHOUK_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFP9898068.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12320..12436
                     /gene="rpmJ"
                     /locus_tag="ACHOUK_002652"
     CDS             12320..12436
                     /gene="rpmJ"
                     /locus_tag="ACHOUK_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFP9898069.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12583..12939
                     /gene="rpsM"
                     /locus_tag="ACHOUK_002653"
     CDS             12583..12939
                     /gene="rpsM"
                     /locus_tag="ACHOUK_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFP9898070.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12956..13345
                     /gene="rpsK"
                     /locus_tag="ACHOUK_002654"
     CDS             12956..13345
                     /gene="rpsK"
                     /locus_tag="ACHOUK_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFP9898071.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13379..13999
                     /gene="rpsD"
                     /locus_tag="ACHOUK_002655"
     CDS             13379..13999
                     /gene="rpsD"
                     /locus_tag="ACHOUK_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFP9898072.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14025..15014
                     /gene="rpoA"
                     /locus_tag="ACHOUK_002656"
     CDS             14025..15014
                     /gene="rpoA"
                     /locus_tag="ACHOUK_002656"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFP9898073.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15055..15438
                     /gene="rplQ"
                     /locus_tag="ACHOUK_002657"
     CDS             15055..15438
                     /gene="rplQ"
                     /locus_tag="ACHOUK_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFP9898074.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15545..15913
                     /locus_tag="ACHOUK_002658"
     CDS             15545..15913
                     /locus_tag="ACHOUK_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFP9898075.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            15924..16349
                     /gene="zntR"
                     /locus_tag="ACHOUK_002659"
     CDS             15924..16349
                     /gene="zntR"
                     /locus_tag="ACHOUK_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFP9898076.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16405..16623
                     /gene="arfA"
                     /locus_tag="ACHOUK_002660"
     CDS             16405..16623
                     /gene="arfA"
                     /locus_tag="ACHOUK_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFP9898077.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(16620..17030)
                     /gene="mscL"
                     /locus_tag="ACHOUK_002661"
     CDS             complement(16620..17030)
                     /gene="mscL"
                     /locus_tag="ACHOUK_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFP9898078.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17160..18536)
                     /gene="trkA"
                     /locus_tag="ACHOUK_002662"
     CDS             complement(17160..18536)
                     /gene="trkA"
                     /locus_tag="ACHOUK_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFP9898079.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18558..19847)
                     /gene="rsmB"
                     /locus_tag="ACHOUK_002663"
     CDS             complement(18558..19847)
                     /gene="rsmB"
                     /locus_tag="ACHOUK_002663"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFP9898080.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKSMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNTSDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRINRTHHSRDTWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19893..20840)
                     /gene="fmt"
                     /locus_tag="ACHOUK_002664"
     CDS             complement(19893..20840)
                     /gene="fmt"
                     /locus_tag="ACHOUK_002664"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFP9898081.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWVEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(20855..21364)
                     /gene="def"
                     /locus_tag="ACHOUK_002665"
     CDS             complement(20855..21364)
                     /gene="def"
                     /locus_tag="ACHOUK_002665"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFP9898082.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21494..22618
                     /gene="dprA"
                     /locus_tag="ACHOUK_002666"
     CDS             21494..22618
                     /gene="dprA"
                     /locus_tag="ACHOUK_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFP9898083.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLARQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLAKHGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22590..23063
                     /gene="smg"
                     /locus_tag="ACHOUK_002667"
     CDS             22590..23063
                     /gene="smg"
                     /locus_tag="ACHOUK_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFP9898084.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23092..23634
                     /locus_tag="ACHOUK_002668"
     CDS             23092..23634
                     /locus_tag="ACHOUK_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFP9898085.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCINYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23639..24211
                     /gene="tsaC"
                     /locus_tag="ACHOUK_002669"
     CDS             23639..24211
                     /gene="tsaC"
                     /locus_tag="ACHOUK_002669"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFP9898086.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24216..25034
                     /gene="aroE"
                     /locus_tag="ACHOUK_002670"
     CDS             24216..25034
                     /gene="aroE"
                     /locus_tag="ACHOUK_002670"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFP9898087.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFV
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKPTADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25031..25288
                     /locus_tag="ACHOUK_002671"
     CDS             25031..25288
                     /locus_tag="ACHOUK_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFP9898088.1"
                     /translation="MNQAIQFPDREEWVENKKCVCFPALVNGMQLTCAISGDSLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25264..25818)
                     /locus_tag="ACHOUK_002672"
     CDS             complement(25264..25818)
                     /locus_tag="ACHOUK_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFP9898089.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6689 a         6178 c         6762 g         6439 t
ORIGIN      
        1 aaagttctgg gctaattaca cgttaattag ttttgaattg aaaagggcgc ttcggcgccc
       61 tttttgctgc ccgtaatttg agctcgaaat atttagtcgt aattttgctc gctgaactat
      121 ggcgcaaata aaactgtgct attgtaatca taactattct catttacact ttgcgcagaa
      181 tttgaacggg agcaatcatg tacgtttgtt tgtgtaatgg tgtaagtgac aaaaaaattc
      241 gccaggcagt ccgtcagttt catccgcaat ctttccaaca attgcgtaag tttattcctg
      301 tgggaaatca atgtggtaag tgcattcgcg ccgcgcgcga agtgatgcag gacgagttga
      361 tgcagatgcc agaatttaaa gagattgcct gaagcccacg cttatttttt gacatcccgc
      421 tagccccatc tacgctctaa tagtggaagc ggagggacta taaaatgaaa ggtgatgtta
      481 aaatcataaa ttatctcaat aaactattgg gaaatgagct tgtcgcaatc aatcagtatt
      541 ttctccatgc ccggatgttt aaaaactggg gactgatgcg tcttaatgac gtcgaatacc
      601 atgaatccat cgatgagatg aaacacgctg ataaatatat cgagcgtatt ttgtttttag
      661 aaggaatccc caacttacag gatttaggta agctagggat cggtgaagat gtcgaagaga
      721 tgctgcaatc cgacctgcga ttagagctgg aaggtgcgaa ggatctgcgt gaggctatcg
      781 cgtatgccga cagcgttcat gactatgtta gccgcgatat gatgatcgag atccttgccg
      841 atgaagaagg tcatatcgac tggctcgaaa ctgaactgga tctcatcggt aaaatcggcc
      901 tacaaaatta cctgcaatca caaattaaag ttaatgatta acggcctatc agcaaactat
      961 cccagcctat aacgaaaccc gcagccgcca gaaatggccc aaaaggcagc gggtttttta
     1021 atgattcttt tcctctcatg agcagaccaa taactacggc tccgcaagcg aatgaggcag
     1081 caaggaaaac cagtcgtggt aagaaggccc atgaatgcca ggcgccaagg gcggcgagaa
     1141 acttcacatc accatagcct aatccttctt tatggcgcag tatgcgatac ccccagtaaa
     1201 tgacagcgaa tgtgccgtaa ccaataattg ccccccataa cgcatcggct aaaccatcag
     1261 gatgacaaac ttgatagaac aaaagacctg accagagtaa cgggcacgta aatctgtcag
     1321 gtaacaagcc atgctttgca tcccagaaaa aaagtagggc tgataaacaa gcgtacaaaa
     1381 ttagaaaggg aagggtagtg tccataaaac atcctctgta attggagtat caccatcgaa
     1441 taactccgga tgctcagcaa cagagagagc gctgatttgt taacctgatt ccggtgaaaa
     1501 acgctatctt cacaacctta caaatttcgt ggaatgcagc tcgcaatctg cgcgttgcga
     1561 ttaatttctg cccgatgctt gcgtcaggct cagatttgcg tataatgcgc gggcttgtcg
     1621 tagttgacag caggttcaat ctgaacctca gttagccgaa tttggctacc taacaatgct
     1681 cccaatcggg gagctacgta agaacggtta cactctccca tcaatcgtaa tgggtctgag
     1741 gagtaatcat tttcgtttat aaaataattg gagctctggt ctcatgcaga accaaagaat
     1801 ccgtatccgc ctgaaagcgt ttgatcatcg tctgatcgat caagcaaccg cggaaatcgt
     1861 cgagactgcc aagcgcactg gtgcgcaggt ccgtggtccg atcccgctgc cgacacgcaa
     1921 agagcgcttc actgttctga tctccccgca cgtcaacaaa gacgcgcgcg atcagtacga
     1981 aatccgtact cacttgcgtc tggttgacat cgttgagcca accgagaaaa ccgttgatgc
     2041 tctgatgcgt ctggatctgg ctgccggtgt agacgtgcag atcagcctgg gttaatcagg
     2101 tcattgagcg attgagaggt tgaaacaatg attggtttag tcggtaaaaa agtgggtatg
     2161 acccgtatct tcacagaaga cggcgtttct atcccagtaa ccgtaatcga agttgaagca
     2221 aaccgcgtta ctcaggttaa agacctggct aacgatggct accgtgctat tcaggtgacc
     2281 accggtgcta aaaaagctaa ccgtgtgacc aagcctgaag ctggccactt cgctaaagct
     2341 ggcgtagaag ctggccgtgg tctgtgggaa ttccgcctgg ctgaaggcga agagttcact
     2401 gtaggtcaga gcattagcgt tgaactgttt gctgacgtta aaaaagttga cgtaactggc
     2461 acctctaaag gtaaaggttt cgcaggtacc gttaagcgct ggaacttccg tacccaagac
     2521 gctactcacg gtaactcctt gtctcaccgc gttccgggtt ctatcggtca gaaccagact
     2581 ccgggcaaag tgttcaaagg caagaaaatg gcaggtcaga tgggtaacga acgtgtaacc
     2641 gttcagagcc ttgacgtagt acgcgttgac gctgagcgca acctgctgct ggttaaaggt
     2701 gctgtcccgg gtgcaaccgg tagcgacctg atcgttaaac cagctgtgaa ggcgtaagga
     2761 gatagcaatg gaattagtat tgaaagacgc gcagagcgcg ctgactgttt ccgaaactac
     2821 cttcggtcgt gatttcaacg aagcgctggt tcaccaggtt gttgttgctt atgcagctgg
     2881 tgctcgtcag ggtactcgtg ctcagaagac tcgtgctgaa gtaactggtt ccggtaaaaa
     2941 accgtggcgc cagaaaggca ccggccgtgc gcgttctggt tctatcaaga gcccgatctg
     3001 gcgttctggt ggcgtgacct ttgctgctcg tccgcaggac cacagtcaaa aagttaacaa
     3061 gaagatgtac cgcggcgcgc tgaaaagcat cctgtccgaa ctggtacgtc aggatcgtct
     3121 gatcgttgtc gagaagttct ctgtagaagc gccgaaaact aagctgctgg cacagaaact
     3181 gaaagacatg gctctggaag atgtgctgat catcaccggt gagctggacg aaaacctgtt
     3241 cctggctgcg cgcaacctgc acaaggttga cgtacgcgat gcaactggta tcgacccggt
     3301 tagcctgatc gccttcgaca aagtcgtaat gactgctgat gctgttaagc aagttgagga
     3361 gatgctggca tgattcgtga agaacgtctg ctgaaggtgc tgcgtgcacc gcacgtttct
     3421 gaaaaagcgt ctactgcgat ggaaaaatcc aacaccatcg tactcaaagt tgctaaagac
     3481 gcgaccaaag cagaaatcaa agctgctgtg cagaaactgt ttgaagtcga agtcgaagtc
     3541 gttaacaccc tggtagttaa agggaaagtt aaacgtcacg gacagcgtat cggtcgtcgt
     3601 agcgactgga aaaaagctta cgtcaccctg aaagaaggcc agaatctgga cttcgttggc
     3661 ggcgctgagt aagtcggagg agtaatacaa tggcagttgt taaatgtaaa ccgacatctc
     3721 cgggtcgtcg ccacgtagtt aaagtggtta accctgagct gcacaagggc aaaccttttg
     3781 ctccgttgct ggaaaaaaac agcaaatccg gtggtcgtaa caacaatggc cgtatcacca
     3841 ctcgtcatat cggtggtggc cacaagcagg cttaccgtat tgttgacttc aaacgcaaca
     3901 aagacggtat cccggcagtt gttgaacgtc ttgagtacga tccgaaccgt tccgcgaaca
     3961 tcgcgctggt tctgtacaaa gacggtgaac gccgttacat cctggcccct aaaggcctga
     4021 aagctggcga ccagattcag tctggcgttg atgctgcaat caaaccaggc aacaccctgc
     4081 cgatgcgcaa catcccggtt ggttctactg ttcataacgt agaaatgaaa ccaggtaaag
     4141 gcggtcagct ggcacgttcc gctggtactt acgttcagat cgttgctcgc gacggtgctt
     4201 atgtcaccct gcgtctgcgt tctggtgaaa tgcgtaaagt cgaagcagac tgccgtgcaa
     4261 ctctgggcga agttggcaat gctgagcata tgctgcgcgt tctgggtaaa gcaggtgctg
     4321 cacgctggcg tggtgttcgt ccgaccgttc gcggtaccgc gatgaacccg gtagaccacc
     4381 cacatggtgg tggtgaaggt cgtaactttg gtaagcaccc ggtaactccg tggggcgttc
     4441 agaccaaagg taagaagacc cgcagcaaca agcgtactga taaattcatc gtacgtcgcc
     4501 gtagcaaata attttagagg ataagccatg ccacgttctc tcaagaaagg tccttttatt
     4561 gacctgcact tgctgaagaa ggtagagaaa gcggtggaaa gcggagacaa gaagcccctg
     4621 cgcacttggt cccgtcgttc aacgatcttt cctaacatga tcggtttgac catcgctgtc
     4681 cataatggtc gtcagcacgt tccggtattt gtaaccgacg aaatggtcgg tcacaaactg
     4741 ggtgaattcg caccgactcg tacttatcgc ggccacgctg ctgataaaaa agcgaagaag
     4801 aaataaggta ggaggaagag atggaaacta tcgctaaaca tcgccatgct cgttcttctg
     4861 ctcagaaggt tcgccttgtt gctgacctga ttcgcggtaa gaaagtgtcg caggctctgg
     4921 atattttgac ctacaccaac aagaaagcgg ctgtactggt caagaaagtt ctggaatctg
     4981 ccattgctaa cgctgaacac aacgatggcg ctgacattga cgatctgaaa gttacgaaaa
     5041 ttttcgtaga cgaaggcccg agcatgaagc gcattatgcc gcgtgcaaaa ggtcgtgcag
     5101 atcgcatcct gaagcgcacc agccacatca ctgtggttgt gtccgatcgc tgagactctg
     5161 gagactagca atgggtcaga aagtacatcc taatggtatt cgcctgggta ttgtaaaacc
     5221 atggaactct acctggtttg cgaacaccaa agaattcgct gacaacctgg acagcgattt
     5281 taaagtacgt cagtacctga ctaaggaact ggctaaagcg tccgtatctc gtatcgttat
     5341 cgagcgtccg gctaagagca tccgtgtaac cattcacact gctcgcccgg gtatcgttat
     5401 cggtaaaaaa ggtgaagacg tagaaaaact gcgtaaggtc gtagcggaca tcgctggcgt
     5461 tcctgcacag atcaacatcg ccgaagttcg taagcctgaa ctggacgcaa aactggttgc
     5521 tgacagcatc acttctcagc tggaacgtcg cgttatgttc cgtcgtgcta tgaagcgtgc
     5581 tgtacagaac gcaatgcgtc tgggcgctaa aggtattaaa gttgaagtta gcggccgtct
     5641 gggcggcgcg gaaatcgcac gtaccgaatg gtaccgcgaa ggtcgcgtac cgctgcacac
     5701 tctgcgtgct gacatcgact acaacacctc tgaagcgcac accacttacg gtgtaatcgg
     5761 cgttaaagtg tggatcttca aaggcgagat cctgggtggt atggctgctg ttgaacaacc
     5821 ggaaaaaccg gctgctcagc ctaaaaagca gcagcgtaaa ggccgtaaat aaggagcgtc
     5881 gctgatgtta caaccaaagc gtacaaaatt ccgtaaaatg cataaaggcc gtaaccgcgg
     5941 tctggcgcag ggtacggatg ttagcttcgg cagcttcggt ctgaaagctg ttggccgtgg
     6001 tcgtctgact gcccgtcaga tcgaagcagc acgtcgtgct atgacccgtg cagttaagcg
     6061 tcaaggtaag atctggatcc gtgtgttccc ggacaaaccg atcactgaaa agccgctggc
     6121 agtgcgtatg ggtaaaggta aaggtaacgt ggagtattgg gttgccttga ttcagccggg
     6181 taaagtcctg tatgaaatgg acggtgttcc ggaagagctg gcccgtgaag cattcaagct
     6241 ggcagcagcg aaactgccga ttaaaaccac ctttgtaact aagacggtga tgtaatgaaa
     6301 gcaaaagagc tgcgtgagaa gagcgttgaa gagctgaaca ccgagctgct gaacctgctg
     6361 cgtgagcagt tcaacctgcg tatgcaggct gcaagtggcc agctgcaaca gtctcacctg
     6421 ttgaagcaag tgcgtcgcga tgtcgcacgc gttaagactt tactgaacga gaaggcgggt
     6481 gcgtaatgac cgataaaatc cgtactctgc aaggtcgcgt tgttagcgac aaaatggaga
     6541 aatccattgt tgttgctatc gaacgttttg tgaaacaccc gatctacggt aaattcatca
     6601 agcgtacgac caaactgcac gtacatgacg agaacaacga atgcggtatc ggtgacgtgg
     6661 ttgaaatccg cgaatgccgt ccgctgtcca agactaaatc ctggacgctg gttcgcgttg
     6721 tagagaaagc ggttctgtaa tacagtacac tctctcgata cgaataaacg gctcagaaat
     6781 gagccgttta ttttttctac ccatatcctt gaagcggtgt tataatgccg cgccctcgat
     6841 atggggattt ttaacgacct gattttcggg tctcagtagt agttgacatt agcggagcac
     6901 taaaatgatc caagaacaga ctatgctgaa cgtcgccgac aactccggtg cacgtcgcgt
     6961 aatgtgtatc aaggttctgg gtggctcgca ccgtcgctac gcaggcgtag gcgacatcat
     7021 caagatcacc atcaaagaag caattccgcg tggtaaggtc aaaaaaggtg atgtgctgaa
     7081 ggcggtagtg gtgcgcacca agaagggtgt tcgtcgcccg gacggttctg tcattcgctt
     7141 cgatggtaat gcttgtgttc ttctgaacaa caacagcgag cagcctatcg gtacgcgtat
     7201 ttttgggccg gtaactcgtg agcttcgtag tgagaagttc atgaaaatta tctctctggc
     7261 accagaagta ctctaaggag cgaatcatgg cagcgaaaat ccgtcgtgat gacgaagtta
     7321 tcgtgttaac cggtaaagat aaaggtaaac gcggtaaagt taagaatgtc ctgtcttccg
     7381 gcaaggtcat tgttgaaggt atcaacctgg ttaagaaaca tcagaagccg gttccggccc
     7441 tgaaccaacc gggtggcatc gttgaaaaag aagccgctat tcaggtttcc aacgtagcaa
     7501 tcttcaatgc ggcaaccggc aaggctgacc gtgtaggctt tagattcgaa gacggcaaaa
     7561 aagtccgttt cttcaagtct aacagcgaaa ctatcaagta atttggagta gtacgatggc
     7621 gaaactgcat gattactaca aagacgaagt agttaaaaaa ctcatgactg agtttaacta
     7681 caattctgtc atgcaagtcc ctcgggtcga gaagatcacc ctgaacatgg gtgttggtga
     7741 agcgatcgct gacaaaaaac tgctggataa cgcagcagca gacctggcag caatctccgg
     7801 tcaaaaaccg ctgatcacca aagcacgcaa atctgttgca ggcttcaaaa tccgtcaggg
     7861 ctatccgatc ggctgtaaag taactctgcg tggcgaacgc atgtgggagt tctttgagcg
     7921 cctgatcact attgctgtac ctcgtatccg tgacttccgt ggcctgtccg ctaagtcttt
     7981 cgacggtcgt ggtaactaca gcatgggtgt ccgtgagcag atcatcttcc cagaaatcga
     8041 ctacgataaa gtcgaccgcg ttcgtggttt ggatattacc attaccacta ctgcgaaatc
     8101 tgacgaagaa ggccgcgctc tgctggctgc ctttgacttc ccgttccgca agtaaggtag
     8161 ggttactaaa tggctaagca atcaatgaaa gcacgcgaag taaaacgcgt agctttagct
     8221 gataaatact tcgcgaaacg cgctgaactg aaagcgatca tctctgatgt gaacgcttcc
     8281 gacgaagatc gttggaacgc tgttctcaag ctgcagactc tgccgcgtga ttccagcccg
     8341 tctcgtcagc gtaaccgctg ccgtcaaaca ggtcgtccgc atggtttcct gcggaagttc
     8401 gggttgagcc gtattaaggt ccgtgaagcc gctatgcgcg gtgaaatccc gggtctgaaa
     8461 aaggctagct ggtaattgtc accaattgaa tcacgggagt aaagacagat gagcatgcaa
     8521 gatccgatcg cggatatgct gacccgtatc cgtaacggtc aggccgcgaa caaagctgcg
     8581 gtcaccatgc cttcctccaa gctgaaagtg gcaatcgcca acgtgctgaa ggaagaaggt
     8641 tttattgaag attttaaagt tgaaggcgac accaagcctg aactggaact tactctgaag
     8701 tatttccagg gcaaagctgt tgtagaaagc attcagcgtg tcagccgccc aggtctgcgc
     8761 atctataaac gtaaagatga gctgccgaaa gttatggcgg gtctgggtat cgcagttgtt
     8821 tctacctcta aaggtgttat gactgatcgt gcagcgcgcc aggctggtct tggtggcgaa
     8881 attatctgct acgtagccta atcggaggaa aaaatgtctc gtgttgctaa agcaccggtc
     8941 gttgttcctg ccggcgttga cgtaaaaatc aacggtcagg ttattacgat caaaggtaaa
     9001 aacggcgagc tgactcgtac tctcaacgat gctgttgaag ttaaacatgc agataatacc
     9061 ctgaccttcg gtccgcgtga tggttacgca gacggttggg cacaggctgg taccgcgcgt
     9121 gccctgctga actcaatggt tatcggtgtt accgaaggct tcactaagaa gctgcagctg
     9181 gttggtgtag gttaccgtgc agcggttaaa ggcaatgtga ttaacctgtc tctgggtttc
     9241 tctcatcctg ttgaccatca gctgcctgcg ggtatcactg ctgaatgtcc gactcagact
     9301 gaaatcgtgc tgaaaggcgc tgataagcag gtgatcggcc aggttgcagc ggatctgcgc
     9361 gcctaccgtc gtcctgagcc ttataaaggc aagggtgttc gttacgccga cgaagtcgtg
     9421 cgtaccaaag aggctaagaa gaagtaaggt aacactatgg ataagaaatc tgctcgtatc
     9481 cgtcgtgcga cccgcgcacg ccgcaagctc caggagctgg gcgcaactcg cctggtggta
     9541 catcgtaccc cgcgtcacat ttacgcacag gtaattgcac cgaacggttc tgaagttctg
     9601 gtagctgctt ctactgtaga aaaagctatc gctgaacaac tgaagtacac cggtaacaaa
     9661 gacgcggctg cagctgtggg taaagctgtc gctgaacgcg ctctggaaaa aggcatcaaa
     9721 gatgtatcct ttgaccgttc cgggttccaa tatcatggtc gtgtccaggc actggcagat
     9781 gctgcccgtg aagctggcct tcagttctaa ggtagaggtg taagatggct cacatcgaaa
     9841 aacaagctgg cgaactgcag gaaaagctga tcgcggtaaa ccgcgtatct aaaaccgtta
     9901 aaggtggtcg tattttctcc ttcacagctc tgactgtagt tggcgatggt aacggtcgcg
     9961 ttggttttgg ttacggtaaa gcgcgtgaag ttccagcagc gatccagaaa gcgatggaaa
    10021 aagcccgtcg caatatgatt aacgtcgcgc tgaataacgg cactctgcaa caccctgtta
    10081 aaggtgttca cacgggttct cgcgtattca tgcagccggc ttctgaaggt accggtatca
    10141 tcgccggtgg tgcaatgcgc gccgttctgg aagtcgctgg ggttcataac gttctggcta
    10201 aagcatatgg ttccaccaac ccgatcaacg tggttcgtgc aactattgat ggcctggaaa
    10261 atatgaattc tccagaaatg gtcgctgcca agcgtggtaa atccgttgaa gaaattctgg
    10321 ggaaataaac catggcaaag actattaaaa ttactcaaac ccgcagtgca atcggtcgtc
    10381 tgccgaaaca caaggcaacg ctgcttggcc tgggtctgcg tcgtattggt cacaccgtag
    10441 agcgcgagga tactcctgct attcgcggta tgatcaacgc ggtttccttc atggttaaag
    10501 ttgaggagta agagatgcgt ttaaatactc tgtctccggc cgaaggctcc aaaaaggcgg
    10561 gtaaacgcct gggtcgtggt atcggttctg gcctcggtaa aaccggtggt cgtggtcaca
    10621 aaggtcagaa gtctcgttct ggcggtggcg tacgtcgcgg tttcgagggt ggtcagatgc
    10681 ctctgtaccg tcgtctgccg aaattcggct tcacttctcg taaagcagcg attacagccg
    10741 aagttcgtct gtctgacctg gctaaagtag aaggcggtgt agtagacctg aacacgctga
    10801 aagcggctaa cattatcggt atccagatcg agttcgcgaa agtgatcctg gctggcgaag
    10861 taacgactcc ggtaactgtt cgtggcctgc gtgttactaa aggcgctcgt gctgctatcg
    10921 aagctgctgg cggtaaaatc gaggaataag tagcagatgg ctaaacaacc gggattagat
    10981 tttcaaagtg ccaaaggtgg cttaggcgag ctgaaacgca gactgctgtt tgttatcggt
    11041 gcgctgattg tgttccgtat tggctctttt attccgatcc ctggtattga tgccgctgta
    11101 cttgccaaac tgcttgagca acagcgaggc accatcattg agatgtttaa catgttctct
    11161 ggtggtgctc tcagccgtgc ttctatcttt gctctgggga tcatgccgta tatttcggcg
    11221 tcgatcatta tccagctgct gacggtggtt cacccaacgt tggcagaaat taagaaagaa
    11281 ggggagtctg gtcgtcgtaa gatcagccag tacacccgtt acggtactct ggtgctggca
    11341 atattccagt cgatcggtat tgctaccggt ctgccgaata tgcctggtat gcaaggcctg
    11401 gtgattaacc cgggctttgc attctacttc accgctgttg taagtctggt cacaggaacg
    11461 atgttcctga tgtggttggg cgaacagatc actgaacgag gtatcggcaa cggtatttca
    11521 atcattatct tcgccggtat tgtcgcggga ctcccgccag ccattgccca tactatcgag
    11581 caagcgcgtc aaggcgacct gcacttcctc gtgttgctgt tggttgcagt attagtattt
    11641 gcagtgacgt tctttgttgt atttgttgag cgtggtcaac gccgcattgt ggtaaactac
    11701 gcgaaacgtc agcaaggtcg tcgtgtctat gctgcacaga gcacacattt accgctgaaa
    11761 gtgaatatgg cgggggtaat cccggcaatc ttcgcttcca gtattattct gttcccggcg
    11821 accatcgcgt catggttcgg gggcggtact ggttggaact ggctgacaac aatttcgctg
    11881 tatttgcagc ctgggcaacc gctttatgtg ttactctatg cgtctgcaat catcttcttc
    11941 tgtttcttct acacggcgtt ggttttcaac ccgcgtgaaa cagcagataa cctgaagaag
    12001 tccggtgcat ttgtaccagg aattcgtccg ggagagcaaa cggcgaagta tatcgataaa
    12061 gtaatgaccc gcctgaccct ggttggtgcg ctgtatatta cctttatctg cctgatcccg
    12121 gagttcatgc gtgatgcaat gaaagtaccg ttctacttcg gtgggacctc actgcttatc
    12181 gttgttgtcg tgattatgga ctttatggct caagtgcaaa ctctgatgat gtccagtcag
    12241 tatgagtctg cattgaagaa ggcgaacctg aaaggctacg gccgataatt ggtcgcccga
    12301 gaagttacgg agagtaaaaa tgaaagttcg tgcttccgtc aagaaattat gccgtaactg
    12361 caaaatcgtt aagcgtgatg gtgtcatccg tgtgatttgc agtgccgagc cgaagcataa
    12421 acagcgccaa ggctgatttt ttcgcatatt tttcttgcaa agttgggttg agctggctag
    12481 attagccagc caatcttttg tatgtctgtg cgtttccatt tgagtatcct gaaaacgggc
    12541 ttttcagcat ggaacgtaca tattaaatag taggagtgca tagtggcccg tatagcaggc
    12601 attaacattc ctgatcataa gcatgccgta atcgcattaa cttcgattta tggcgtcggc
    12661 aagacccgtt ctaaagccat cctggctgca gcgggtatcg ctgaagatgt taagatcagt
    12721 gagctgtctg aaggacaaat cgacacgctg cgtgacgaag ttgccaaatt tgtcgttgaa
    12781 ggtgatctgc gccgtgaaat cagcatgagc atcaagcgcc tgatggatct tggttgctat
    12841 cgcggtttgc gtcatcgtcg tggtctcccg gttcgcggtc agcgtaccaa gaccaacgca
    12901 cgtacccgta agggtccgcg caaaccgatc aagaaataat cggggtgatt gaataatggc
    12961 aaaggcacca attcgtgcac gtaaacgtgt aagaaaacaa gtctctgacg gcgtggctca
    13021 tatccatgct tctttcaaca acaccatcgt gactatcact gatcgtcagg gtaacgcgtt
    13081 gggttgggca acagccggtg gttccggttt ccgtggttct cgcaaatcca ctccgtttgc
    13141 agctcaggtt gcagcagagc gttgcgctga cgccgtgaaa gaatacggca tcaagaatct
    13201 ggaagttatg gttaaaggtc cgggtccagg ccgcgaatct actattcgtg ctctgaacgc
    13261 cgcaggtttc cgcatcacta acattactga tgtgactccg atccctcata acggttgtcg
    13321 tccgccgaaa aaacgccgcg tataacgcct cgttttccag gtttgttgga gaaagaaaat
    13381 ggcaagatat ttgggtccta agctcaagct gagccgtcgt gagggcaccg acttattcct
    13441 taagtctggc gttcgcgcga tcgataccaa gtgtaaaatt gaacaagctc ctggccagca
    13501 cggtgcgcgt aaaccgcgtc tgtctgacta tggtgtgcag ttgcgtgaaa agcaaaaagt
    13561 tcgccgtatc tatggtgtgc tggagcgtca gttccgtaac tactacaaag aagcagcacg
    13621 tctgaaaggc aacaccggtg aaaacctgtt ggctctgctg gaaggtcgtc tggacaacgt
    13681 tgtataccgt atgggcttcg gtgccactcg tgcagaagca cgtcagctgg ttagccataa
    13741 agcaattatg gtaaacggtc gtgttgttaa catcgcttct tatcaggtta gtccgaatga
    13801 cgttgtaagc attcgtgaga aagcgaagaa gcagtctcgc gtgaaagccg ctctggagct
    13861 ggctgagcag cgtgaaaagc caacctggct ggaagttgat gctggcaaga tggaaggtac
    13921 gtttaagcgt aagccggagc gttctgatct gtctgcggac attaacgaac acctgatcgt
    13981 cgagctttac tccaagtaaa gcttagtacc aaagagagga cacaatgcag ggttctgtga
    14041 cagagtttct aaaaccgcgc ctggttgata tcgagcaagt gagttcgacg cacgccaagg
    14101 tgacccttga gcctttagag cgtggctttg gccatactct gggtaacgca ctgcgccgta
    14161 ttctgctctc atcgatgccg ggttgcgcgg tgaccgaggt tgagattgat ggtgtactac
    14221 atgagtacag caccaaagaa ggcgttcagg aagatatcct ggaaatcctg ctcaacctga
    14281 aagggctggc ggtgagagtt cagggcaaag atgaagttat tcttaccttg aataaatctg
    14341 gcattggccc tgtgactgca gccgatatca cccacgacgg tgatgtcgaa atcgtcaagc
    14401 cgcagcacgt gatctgccac ctgaccgatg agaacgcgtc tattagcatg cgtatcaaag
    14461 ttcagcgcgg tcgtggttat gtgccggctt ctacccgaat tcattcggaa gaagatgagc
    14521 gcccaatcgg ccgtctgctg gtcgacgcat gctacagccc tgtggagcgt attgcctaca
    14581 atgttgaagc agcgcgtgta gaacagcgta ccgacctgga caagctggtc atcgaaatgg
    14641 aaaccaacgg cacaatcgat cctgaagagg cgattcgtcg tgcggcaacc attctggctg
    14701 aacaactgga agctttcgtt gacttacgtg atgtacgtca gcctgaagtg aaagaagaga
    14761 aaccagagtt cgatccgatc ctgctgcgcc ctgttgacga tctggaattg actgtccgct
    14821 ctgctaactg ccttaaagca gaagctatcc actatatcgg tgatctggta cagcgtaccg
    14881 aggttgagct ccttaaaacg cctaaccttg gtaaaaaatc tcttactgag attaaagacg
    14941 tgctggcttc ccgtggactg tctctgggca tgcgcctgga aaactggcca ccggcaagca
    15001 tcgctgacga gtaaccggat cacaggttaa ggttttactg agaaggataa ggtcatgcgc
    15061 catcgtaaga gtggtcgtca actgaaccgc aacagcagcc atcgccaggc tatgttccgc
    15121 aatatggcag gttcactggt tcgtcatgaa atcatcaaga cgactctgcc taaagcgaaa
    15181 gagctgcgcc gcgtagttga gccgctgatt actcttgcca agactgatag cgttgctaat
    15241 cgtcgtctgg cattcgcccg tactcgtgat aacgagatcg tggcaaaact gtttaacgaa
    15301 ctgggcccgc gtttcgcgag ccgtgccggt ggttacactc gtattctgaa gtgtggcttc
    15361 cgtgcaggcg acaacgcgcc gatggcttac atcgagctgg ttgatcgttc agagaaagca
    15421 gaagctgctg cagagtaatc tgaagcaacg taaaaaaacc cgccccggcg ggttttttta
    15481 tacccgtagc atccccactt atctacaata gctgtactct ttttgttcat cccctggagt
    15541 atttatgtgg ttacttgacc agtgggcaga gcgccatata gcagaagcgc aagcgaaagg
    15601 tgagtttgat aacctggcag gtagcggcga accattgata ctggatgatg attctcatgt
    15661 gccaccggaa ttacgtgcgg ggtatcgctt gctgaagaat gccggttgct taccgccaga
    15721 acttgagcaa cggagggaag caatccagct tctggatatc ctcaaaggta tccgtcacga
    15781 tgatccgcaa tatcaagagg ttagccgtcg attgtcatta ctggaattga agctgcgaca
    15841 agctggattg agtaccgatt ttttacgcgg cgattatgct gacaagttgt tgaataaaat
    15901 caacgataac tagtggagta tgtatgtatc gcattggtga gctggcaaaa atggcggaag
    15961 taacacccga cacgattcgt tattacgaaa agcagcagat gatggagcat gaagtgcgta
    16021 ccgaaggtgg gtttcgcctg tataccgaaa gcgatctcca gcgattgaaa tttatccgcc
    16081 atgccagaca actaggtttc agtctggagt cgatccgcga gttgctgtcg atccgtatcg
    16141 atcctgaaca ccatacctgt caggagtcaa aaggcattgt gcaggaaaga ttgcaggaag
    16201 tcgaagcacg gatagccgag ttgcagagta tgcagcgttc cttgcaacgc cttaacgatg
    16261 cctgttgtgg gaccgcccat agcagtgttt attgctcgat tcttgaagct cttgaacaag
    16321 gggcgagtgg cgttaagagt ggctgttgat tttttgcact ggcaggatta cactcacgcc
    16381 gttaaataac caactggagt ttttatgagc cgatatcagc atactaaagg gcagataaag
    16441 gataatgcga tagaagcatt actacatgat cccttattcc gacagcgcgt agagaaaaat
    16501 aagaagggga aaggcagtta catgcgaaaa ggaaaacatg gcaatcgggg gaactgggag
    16561 gccagtggca agaaagtaaa tcactttttt accactggtc ttctgctttc aggggcttgt
    16621 taagagcggt tattctgctc tttcagcaaa tcacgaattt ctgtcagtaa tacttcttct
    16681 ttagttggtg ctggtgcggc tgctggttct tcttttttcc gattcagttt gttgattagc
    16741 ttaatcgcca taaagatggc aaaggccaca atcagaaaat caaagacgtt ttgaatgaag
    16801 acaccgtaat gcatcacaac agcagggata tccccctgcg catcgcgtag cgtgacagca
    16861 aactgtttaa agtcgatccc gccaattaat aagcccagtg gtggcatgat gatatcggca
    16921 accagtgaag atacaatctt cccgaatgcc gcaccgataa tgacacccac cgccaaatcc
    16981 accacgttcc cgcgcatcgc aaattcgcga aattctttaa taatgctcat gttattctcc
    17041 ctatgaagcc gacaaccata agtctaacaa atgttaagcc attttcctgc caggaatgaa
    17101 tttaattaat ccttgataat caaagggaaa taataaggcg tcattagacg ccttattaat
    17161 tacaagaaga aagggcttgg ctggaagagt ctttcgacgt cggtaataaa ttttttatct
    17221 gtgaggaaca taattacgtg atcgccttgc tcaatgcgca gattgtcatt ggcaatcatc
    17281 acgtcgtttc cacgtaccac cgctccaata atcgttcctg gcggtagctt gatttcgtca
    17341 atgactctgc cgacaacgcg tgaggtgctt tcatcaccgt gagcaacggc ttcaatagct
    17401 tctgctacgc cgcggcgcaa tgaggaaaca ccaacaatat ctgcttttcg cacatggcta
    17461 agcaacgcag aaatagttgc ttgttgtggt gaaatcgcaa tatcgatcac gctcccctga
    17521 accagatcca cataagcgcg acgctggatc aataccatca cctttttcgc acccatacgt
    17581 ttggcaagca tggcggacat gatattggcc tcgtcatcgt tggtgacggc aataaacaga
    17641 tcaacttgat cgatatgttc ttcggccagc agttcttgat ccgacgcatc accaaaaaag
    17701 acgatcgtat tctgcaactt ttccgccagt tcggcagcgc gctgctgatt acgttcgatg
    17761 agtttaacgc tgtaatcttt ttccagacga cgcgccagcc ctgcaccgat attaccgcca
    17821 ccaaccagca tgatccgctt atacggtttt tccagtcgct gtaattcact catcaccgcg
    17881 cggatatgct gtgaagcggc aataaagaac acttcatcac cagcctcaac aatggtcgaa
    17941 ccttgcggac gaatggggcg atcgtggcgg aaaattgctg ccacacgagt atcgatatgt
    18001 ggcatatgtt cgcgcatggt cgaaagtgca ttaccaatca gcgggccgcc ataataggct
    18061 ttaaccacag cgaggctgac tttaccctca gcgaagttca ccacctgcaa tgcgccggga
    18121 tactcaatca gtcggtaaat attatcgata accaactgct ctggtgcgat cagatgatca
    18181 atcggtacag catctgaatg aaatagctta tcggcatcgc gtacgtagtc tggtgagcgg
    18241 atacgagcga tgcgattagg ggtgttgaaa agtgagtagg ctacctggca ggcaaccata
    18301 ttggtttcat ctgaactggt tacagcaacc agcatatcgg cgtcgtcggc acctgcctcc
    18361 cgcaatacgc gtggatgaga gccatgcccc tgcacgactc gcaggtcaaa tttatcctgc
    18421 aaggttcgca gacgctcacc gttggtatcg acaacagtga tatcgttgtt ctcgccaacc
    18481 aggttttccg ccagtgtgcc gccaacctgg ccggcaccca gaatgataat tttcatcagt
    18541 cgcgacccgt tatctcatca ctttttgatt agcttagcgt aaaagaagcc gtcgccctct
    18601 tcggcaccag gcagattttg tttacccggt tgctctggtg ttcctgtttc gcaaagttcg
    18661 gcatcagcgg tacgttgcaa aaaggcttta atctgcaggc tattctcttc cggtaacacc
    18721 gaacaggtgg catagaccag ggttccaccg gattttaaat gcggccaaat ggcgtcgaga
    18781 atttcagact gcaattgcgc gagttctggg atatcgcgat cgcgacgtaa ccatttaata
    18841 tccggatggc gacgaatcac accggttgct gaacaaggcg catctaataa aatgcgatca
    18901 aactgttgct cgccacacca ttgggaaggg taacggccat caccttgttt cacggttgcc
    18961 ttcataccaa ggcgttttaa attgtcgtaa acgcgagaga ggcgctgttc gtcaatatca
    19021 accgcaacaa cctgcgcttc tggtgccact tcaaggatat gcgttgtttt accgccgggg
    19081 gcggcacaaa gatccagaat gtgttcaccg ttttgtggcg caagccaggt catgcaacct
    19141 tgtgctgatg catcctgaac ggtaacccat ccgtcttcaa aaccaggtag cgcatgaaca
    19201 ggtgcaggtg tttccagccg tacagcatca gggtaatccg catgcgggaa acctttcatt
    19261 cctgcttcat ccagcaatgc aagccaggtg tcgcgggaat gatgcgtacg attaatacgc
    19321 agccacattg gcggacgctg gttattggct tcgacgatgg attgccactg ctctggatac
    19381 gctttttgca gacgcttcag caaccaggaa ggatgcagat aacgtgcatc actggtatta
    19441 aactcggcta ataactcttc ttgctgacgc tggaactggc gtaatacacc gttaatcaac
    19501 cctttaagtt gcggacgctt aattgcgata gcgccttcaa ccgtttcagc cagcgcagca
    19561 tgaggtggaa tgcgggtata aagcagttga tacaaaccaa ccataatcag gtaatgcacg
    19621 gtccgctgtt tgccggtcat cggacgggcc attgacttat taatcagcca gtctaactgc
    19681 gaaagcgtac gcagtacgcc aaagcacaac tcttgaagaa gtgctttgtc tttatcggaa
    19741 actttttgct ggagcggtgg cagaatgttg cttaatgatt gcccttgctc gacgacttgt
    19801 tcaacggcct gggccgccat gctacgtaaa ttacgttgtt ttttcataag tataaaaatg
    19861 cccggcaaga ccgggcttag aagagtggac tatcagacca gacggttgcc cggaacaaac
    19921 cattcccgac gagagttcag gaggtcttgc gcgctcatcg ctttcttacc cgcaggttgt
    19981 aacgagagca ggttcaggat gccatcacca gtcgcaacct gaatgccttg tttgttggct
    20041 tcaaggatcg ttcctggtgc agcgttggtt gccgtatcaa tgaccgatgc tttccagact
    20101 ttaaccggct gtccttcaat ttccacccag ctcattggcc atggattgaa agcgcgaatg
    20161 cagcgttcaa gctgtgctgc cgaaagtgac cagtcaatac gcgcttcttc tttactcaac
    20221 ttctcggcgt aagtgacaag agtttcgtcc tgaacttctg gtttcgccgt gccgtctgcc
    20281 aattgtttca acgtggtgat aagcccttgt gggccaagct ctgccagctt gtcgtacagc
    20341 gtaccactgg tatcttctgc agtaatcggg caggagagct tatagagcat gtcaccggtg
    20401 tctaaaccaa catccatttg cataatggtc acaccagttt ctgcatcacc cgcccatagt
    20461 gagcgttgga ttggtgcagc accgcgccag cgtggcagca gtgaaccatg aacgttgata
    20521 cagccaagac gcggcatctc cagcactgct ttaggcagaa ttaagccata ggcgacgaca
    20581 accataacat cagcctgtag atcggcgacc agttgctggt tttcttgtgg acgcagggaa
    20641 acaggttgaa aaacgggcag acctttttcc tcagccagaa ctttaaccgg gctgggcatc
    20701 agttttttac cgcgtcctgc cggtcggtct ggctgggtga acacgccaac gacgttatga
    20761 ccagaagaca acagcgcgtc gagatgacgc gctgcaaagt caggtgtacc cgcaaaaata
    20821 atacgtagtg attctgacac gttagttctt atccttaagc ccgggctttc agacgatcca
    20881 gtttttcaac tttctgacga atacgttgtt gtttcagcgg tgacagataa tccataaaca
    20941 gtttgccgac caggtgatcc atctcatgct gaatacagat ggctaacaga ccatctgcct
    21001 ccagttcaaa tggtttaccg tcgcggtcca gggcgcgaat tttaactttc tctgcgcgtg
    21061 gcactaaagc acgttgttca gggatcgaca ggcaaccttc ttcaatgcct gtttcgccgc
    21121 ttttttctaa aagctccgga ttgattaaca ccagccgttc gtcacggttt tccgaaacat
    21181 caataacaat gatacgttga tggatatcaa cctgggttgc cgccaggcca ataccttctt
    21241 ctgcgtacat cgtctcgaac atatcatcga cgatacgctg aatttctgca ttcacttctt
    21301 ctaccggttt agcaactttg cgaagccgct cgtccggaat atgtaacact tgcaaaactg
    21361 acataaatct ccagagatgt gttcaggagt tagaaagatt atttcttcta ttctagacaa
    21421 atccccctct gattgacagc atcactgacc aatcgcaaag attgctaagg ctgcttatgg
    21481 cagggagata aggatggtcg atacagaaat ttggctgcgt ttaatgagta tcagcagctt
    21541 gtacggcgat gatatggtcc gtatagctca ctggctggca agacagtcgc atattgatgc
    21601 ggttgtattg cagcaaacag ggcttacatt gcggcaggca caacgctttc tttcatttcc
    21661 gcggaagagt atcgaaagct cactttgttg gttggagcaa cccaaccatc atttaatccc
    21721 tgcggacagc gaattttatc ctcctcaact tctggcgacg acagattacc ccggtgcact
    21781 gtttgttgaa ggagaactgc acgcgctgca ttcatttcag cttgccgtag tggggagtcg
    21841 ggcgcattca tggtatggcg agcgatgggg acgattattt tgcgaaactc tggcgaagca
    21901 tggagtgaca attacgagtg gactggcgcg tggaatcgat ggtgtagcgc ataaagcagc
    21961 cttacaggta aatggcgtca gcattgctgt attggggaat ggacttaata ccattcatcc
    22021 ccgccgtcat gcccgactgg ctgccagtct gcttgaacag gggggcgctc tcgtctcgga
    22081 atttcccctc gatgttccac cccttgctta caatttccca cgaagaaatc gcattatcag
    22141 tggtctaagt aaaggtgtac tggtggtgga agcggctttg cgtagtggtt cgctggtgac
    22201 agcacgttgt gcgcttgagc aggggcgaga agtttttgcc ttgccaggtc caatagggaa
    22261 tccgggaagc gaagggcctc actggttaat aaaacaaggt gcgattcttg tgacggaacc
    22321 ggaagaaatt ctggaaaact tgcaatttgg attgcactgg ttgccagacg cccctgaaaa
    22381 ttcattttat tcaccagatc aggaagacgt ggcattgcca tttcctgagc tcctggctaa
    22441 cgtaggagat gaggtaacac ctgttgacgt cgtcgctgaa cgtgccggcc aacctgtgcc
    22501 agaggtagtt actcaactac tcgaactgga gttagcagga tggatcgcag ctgtacccgg
    22561 cggctatgtc cgattgagga gggcatgcca tgttcgacgt actaatgtat ttgtttgaaa
    22621 cctatattca cacagaagct gagttgcgtg tggatcaaga caaacttgaa caggatctta
    22681 ccgacgcagg atttgatcga gaagatatct acaatgccct gctttggctg gaaaagcttg
    22741 cggattatca ggaaggactg gcagaaccga tgcaactggc ctctgatcca ctctccatgc
    22801 gtatttatac ccctgaggag tgtgaaagac tggatgccag ctgtcgtggt tttctgcttt
    22861 tccttgagca aattcaggtg cttaaccttg aaacccgtga aatggtgata gagcgagtgc
    22921 tcgcgctgga taccgcagag ttcgacctgg aagacctgaa atgggtaatc ctgatggtgt
    22981 tgttcaatat tccgggctgc gaaaatgcgt accagcaaat ggaagaatta ctctttgaag
    23041 tgaatgaagg tatgctgcat taatcatttc tttaattcag cataagttgt tatggcgaaa
    23101 tcagcactgt tcacggtgcg taataatgag tcctgcccaa agtgcggggc tgaactggtt
    23161 attcgatccg ggaaacacgg tccgtttctt ggatgctcac agtatccggc gtgtgactac
    23221 gtccgtcctc tgaaatcttc agcggatgga catatcgtca aagttctgga ggggcaggtt
    23281 tgccctgcat gtggcgcaaa tctggtatta cgccagggac gctttggtat gtttattggt
    23341 tgcattaact accctgaatg cgaacatacc gaacttatcg ataaaccgga cgaaacagca
    23401 attacatgtc cccaatgccg gacgggccat ctggtccagc gccgctcccg ttatggcaaa
    23461 acatttcact cctgtgatcg ctacccggag tgtcaatttg ccattaactt caaacccata
    23521 gctggagaat gccctgagtg tcattatccg ctactcatcg aaaagaaaac cgcgcagggt
    23581 gtaaaacact tttgtgccag taaacaatgt ggaaagccgg tttcggcgga ataataacgt
    23641 gaataataac ctgcaaggag acgctatcgc agctgcgata gatgttctca atgaagaacg
    23701 tgtcatcgcc tatccaacgg aagccgtttt cggtgttggg tgcgatcctg atagcgaaac
    23761 agcagtgatg cgactgttgg agttaaaaca gcgtccggtt gataaggggc tgattttaat
    23821 cgcagcaaat tacgagcagc ttaaacccta tattgatgac accatgttga ctgacgtgca
    23881 gcgtgaaacc attttttccc gctggccagg tcctgtcacc tttgtctttc ccgcgcctgc
    23941 gacaacaccg cgctggttga cgggccgctt tgattcgctt gctgtacgag tcaccgacca
    24001 tccgttggtg gttgctttgt gccaggctta tggtaaaccg ctggtttcta ccagtgccaa
    24061 cttgagtgga ttgccacctt gtcgaacagt agacgaagtt cgcgcacaat ttggcgcggc
    24121 gttcccggtt gtgcctggtg aaacgggggg gcgtttaaat ccttcagaaa tccgcgatgc
    24181 cctgacgggt gaactgtttc gacaggggta acataatgga aacctatgct gtttttggta
    24241 atccgatagc ccacagcaaa tcgccattca ttcatcagca atttgctcag caactgaata
    24301 ttgaacatcc ctatgggcgc gtgttggcac ccatcaatga tttcgtcaac acactgaacg
    24361 ctttctttag tgctggtggt aaaggtgcga atgtgacggt gccttttaaa gaagaggctt
    24421 ttgccagagc ggatgagctt actgaacgcg cagcgttggc tggtgctgtt aacaccctca
    24481 agcggttaga agatggacgc ttgctgggtg acaataccga tggtgtaggc ttgttaagcg
    24541 atctggaacg tctgtctttt atccgtcctg gtttacgtat tctgctcatc ggcgcaggtg
    24601 gagcatctcg cggcgtacta ctgccacttc tttccctgga ctgtgcggtg acaatcacta
    24661 atcggacggt atcccgagcg gaagagttgg ctaaattgtt tgcgcacact ggcagtattc
    24721 aggcgttggg tatggacgaa ctggaaggtc atgagtttga tctcattatt aatgccacat
    24781 ccagtggcat cagtggtgat attccggcga tcccgtcatc gctcattcat ccaggcattt
    24841 attgctatga catgttctat cagaaaggaa aaactccttt tttggcatgg tgtgagcagc
    24901 gaggctcaaa gcctactgct gatggtttag gaatgctggt ggcacaggcg gctcatgcct
    24961 tccttctttg gcatggcgtt ctgcctgatg tagaaccagt tataaagcaa ttgcaggagg
    25021 aattgtccgc gtgaatcagg ccatccagtt tccggacaga gaagagtggg tcgagaataa
    25081 aaaatgtgta tgttttcccg ctctcgtgaa tggcatgcaa ctgacatgtg cgatctctgg
    25141 cgatagcctg gcgtatcgct ttacaggaga tacgccagag cagtggttag cgagttttcg
    25201 tcagcatcgc tgggacttgg aagaagaagc ggaaaactta attcaggaac aaagtgaaga
    25261 tgatcaaggc tgggtctggt taccctgatc cagatattcg tccttccatt tcacgtaatt
    25321 attcgcggaa tagcgtaacc cagccttctc ttcatcactt aacgggcgga tctgtttgac
    25381 gggactaccg agatacagat atccgctctc cagccgttta ttttgtggga ccagactacc
    25441 cgcaccaatc atcacatcat cttctactat tgcgccatca agtaaaattg agcccatccc
    25501 aaccaaaact cgattgccaa tggtgcagcc gtggagcatc accttgtgac caacagtgac
    25561 atcttcgcca atggttaatg ggttgccatc tgggttgtac gaggatttat gagtgacatg
    25621 caacatactg ccatcctgga tattggtgcg tgctccgatc tgtacataat gtacatctcc
    25681 acgaatcaca acgagcggcc agatccccac atcatcagcc agacgaacgt caccaatcac
    25741 gacactgcta tcgtcgatca ttacgcgctg accgatttgt ggaaaaagat cgcggtatgg
    25801 gcgtaaaaca tcagacatac ttacctcagc aataaatgat ttactaatga ctttgggggc
    25861 attattggcc ttgtgcaagt cttttagtat gcaaaaaagc accgttttgt gtgcgaatgc
    25921 agcaaaaagg gtgaaaaaac aacaaacaga aaaaaagatc aaaaaagtac ttgtgcaaaa
    25981 aattgggatc cctataatgc gcctccgttg agacgacaac gtgaaacact tcacaagatg
    26041 gtcgtaacaa cgaagagaaa aaatcctg
//
LOCUS       DAZKIP010000059        25641 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZKIP010000059 DAZKIP010000000
VERSION     DAZKIP010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.059"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            387..1526
                     /gene="wza"
                     /locus_tag="ACHOUK_002673"
     CDS             387..1526
                     /gene="wza"
                     /locus_tag="ACHOUK_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFP9898090.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            1532..1975
                     /gene="wzb"
                     /locus_tag="ACHOUK_002674"
     CDS             1532..1975
                     /gene="wzb"
                     /locus_tag="ACHOUK_002674"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFP9898091.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            1978..4140
                     /gene="wzc"
                     /locus_tag="ACHOUK_002675"
     CDS             1978..4140
                     /gene="wzc"
                     /locus_tag="ACHOUK_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFP9898092.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMEDQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQILKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            4233..5072
                     /gene="wcaA"
                     /locus_tag="ACHOUK_002676"
     CDS             4233..5072
                     /gene="wcaA"
                     /locus_tag="ACHOUK_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFP9898093.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            5075..5563
                     /gene="wcaB"
                     /locus_tag="ACHOUK_002677"
     CDS             5075..5563
                     /gene="wcaB"
                     /locus_tag="ACHOUK_002677"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFP9898094.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            5560..6777
                     /gene="wcaC"
                     /locus_tag="ACHOUK_002678"
     CDS             5560..6777
                     /gene="wcaC"
                     /locus_tag="ACHOUK_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFP9898095.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQALFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            6752..7969
                     /gene="wcaD"
                     /locus_tag="ACHOUK_002679"
     CDS             6752..7969
                     /gene="wcaD"
                     /locus_tag="ACHOUK_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFP9898096.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKITQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGICAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            7980..8726
                     /gene="wcaE"
                     /locus_tag="ACHOUK_002680"
     CDS             7980..8726
                     /gene="wcaE"
                     /locus_tag="ACHOUK_002680"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFP9898097.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAAYF
                     VRKLKMQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            8742..9290
                     /gene="wcaF"
                     /locus_tag="ACHOUK_002681"
     CDS             8742..9290
                     /gene="wcaF"
                     /locus_tag="ACHOUK_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFP9898098.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYVWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRKRVETE"
     gene            9319..10440
                     /gene="gmd"
                     /locus_tag="ACHOUK_002682"
     CDS             9319..10440
                     /gene="gmd"
                     /locus_tag="ACHOUK_002682"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFP9898099.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            10443..11408
                     /gene="fcl"
                     /locus_tag="ACHOUK_002683"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             10443..11408
                     /gene="fcl"
                     /locus_tag="ACHOUK_002683"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFP9898100.1"
                     /translation="MSRQRIFIAGHRGMVGSAIRRQLEQRGDVEQVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            11411..11890
                     /gene="gmm"
                     /locus_tag="ACHOUK_002684"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             11411..11890
                     /gene="gmm"
                     /locus_tag="ACHOUK_002684"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFP9898101.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            11887..13110
                     /gene="wcaI"
                     /locus_tag="ACHOUK_002685"
     CDS             11887..13110
                     /gene="wcaI"
                     /locus_tag="ACHOUK_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFP9898102.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            13113..14549
                     /gene="cpsB"
                     /locus_tag="ACHOUK_002686"
     CDS             13113..14549
                     /gene="cpsB"
                     /locus_tag="ACHOUK_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFP9898103.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPENDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEDAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            14742..16112
                     /gene="cpsG"
                     /locus_tag="ACHOUK_002687"
                     /gene_synonym="manB"
     CDS             14742..16112
                     /gene="cpsG"
                     /locus_tag="ACHOUK_002687"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFP9898104.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDARLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPSSGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            16167..17561
                     /gene="wcaJ"
                     /locus_tag="ACHOUK_002688"
     CDS             16167..17561
                     /gene="wcaJ"
                     /locus_tag="ACHOUK_002688"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFP9898105.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKLGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            17563..19041
                     /gene="wzxC"
                     /locus_tag="ACHOUK_002689"
     CDS             17563..19041
                     /gene="wzxC"
                     /locus_tag="ACHOUK_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFP9898106.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDLI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYVLGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            19221..20501
                     /gene="wcaK"
                     /locus_tag="ACHOUK_002690"
     CDS             19221..20501
                     /gene="wcaK"
                     /locus_tag="ACHOUK_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFP9898107.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQVYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGVMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLSEAVSRERQTGMQMVQSVLERIGEVK"
     gene            20498..21718
                     /gene="wcaL"
                     /locus_tag="ACHOUK_002691"
     CDS             20498..21718
                     /gene="wcaL"
                     /locus_tag="ACHOUK_002691"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFP9898108.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THVAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESW
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGVIHGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            21729..23123
                     /gene="wcaM"
                     /locus_tag="ACHOUK_002692"
     CDS             21729..23123
                     /gene="wcaM"
                     /locus_tag="ACHOUK_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFP9898109.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTI
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITQDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGNYLSIPQNF
                     RVNNIQLDNTHLAYKLRGLQISAGNAVSFVALTNIEMKRASLELHNKPQHLFLRNIKV
                     MQESSVGPALIMNFDMRKDVRGVFMAKEETLLSLANVHAVNEKGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            23281..24276
                     /locus_tag="ACHOUK_002693"
     CDS             23281..24276
                     /locus_tag="ACHOUK_002693"
                     /EC_number="5.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,
                     octacis-undecaprenol 4-epimerase"
                     /protein_id="HFP9898110.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            24519..25412
                     /gene="galF"
                     /locus_tag="ACHOUK_002694"
     CDS             24519..25412
                     /gene="galF"
                     /locus_tag="ACHOUK_002694"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFP9898111.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
BASE COUNT         6330 a         6352 c         6919 g         6040 t
ORIGIN      
        1 aattgttacc ttgcctgcta ttccgttagc tgtaacactt cctcctgcat tattggagag
       61 ccaatattca attaccataa tgtcacctta tattcggaag ttaatttccg caataaccct
      121 ttctcatcaa cgattgtatg cagagagggt ttgaaaaatt aagctaaatc agtgtattgg
      181 tagctaaaaa gccaggggcg gtagcgtgtc tggatgcctg aaagaccagt ctgaattatt
      241 ctgtcaatag cctgcggatg aaatcaattt ttttaggact gatgccagtt aaatttttat
      301 tcaattaact gcaaggtaaa tgtgcatggc gacattgcta ttaatagtgc acaggataat
      361 tactctgcca aagtgataaa taaacaatga tgaaatccaa aatgaaattg atgccattat
      421 tggtgtcagt aaccttgata agcggttgca cagtacttcc gggcagcaat atgtcgacga
      481 tgggcaaaga cgtcatcaaa cagcaggacg ctgatttcga tctcgacaaa atggtgaatg
      541 tttatccgct gaccccgcgc ctgattgacc aattacgccc acgcccgaat gtagcgcgcc
      601 ccaatatgac gctggaaagt gagatcgcga attaccagta tcgcgtcggg ccgggggacg
      661 ttcttaatgt caccgtctgg gatcacccgg aactcaccac gccagccggt cagtaccgca
      721 gctccagcga caccggcaac tgggtacagc ctgacggcac tatgttttac ccgtatatcg
      781 gcaaggttca cgtagtcggg aaaacgctcg ctgaaatccg cagtgatatt accgggcgct
      841 tagcgacgta catcgctgac ccgcaggtgg acgttaatat cgccgccttc cgctcacaaa
      901 aggcctatat ctcaggtcag gtgaataaat ctggtcaaca ggcgatcacc aacgtaccac
      961 tgaccattct cgacgccatc aacgccgcag gtggcctgac cgacaccgct gactggcgca
     1021 acgtggtgct aacacacaat ggtcgtgaag agcgaatttc tttgcaggcg ctgatgcaaa
     1081 acggcgacct caatcagaac cgactgcttt accccggcga tattctctac gtgccgcgta
     1141 atgatgatct gaaagtgttt gtgatgggtg aagtgaagaa acagagcacc ctgaaaatgg
     1201 actttagcgg catgaccctg accgaagccc tgggcaatgc tgaaggcatc gacatgacca
     1261 cctccaacgc cagcggcatc tttgtcattc gtccgctgaa aggcgagggc gggcgtaacg
     1321 gcaaaattgc caatatctac cagctggata tgtccgatgc cacgtcgctg gtgatggcga
     1381 cagaattccg cctgcaacct tatgacgtgg tgtatgtcac caccgccccg gtttcccgct
     1441 ggaaccgtct gatcaatcag ttgctgccaa ctattagcgg tgtccgttac atgacggata
     1501 cagccagcga cattcataac tggtaatcgt catgtttaac aacatcttag ttgtctgtgt
     1561 cggcaatatt tgccgttccc cgacggcgga acgcttactg caacgttatc acccggagct
     1621 gaaagtggag tccgctggac tcggcgcgct ggtcggtaag ggcgctgatc ctaccgctat
     1681 cagcgtcgcc gcagaacatc aactgtctct ggaaggccac tgtgcacgac aaatcagccg
     1741 ccgcctgtgt cgcaactatg acctgatttt gaccatggaa aagcgccata tcgaacgctt
     1801 atgcgagatg gcaccggaga tgcgcggcaa agtgatgctg tttggtcact gggataacga
     1861 atgtgaaatc cccgatccgt atcgcaaaag ccgggaaacg tttgcagcgg tgtacacatt
     1921 acttgaacgg tctgcccgcc agtgggcgca ggcattgaac gcagagcagg tataagaatg
     1981 acagaaaaag taaaacaaca tgccgctccg gtaacgggca gtgatgaaat cgatattggt
     2041 cgcctggtcg gcaccgtcat tgaagcgcgc tggtgggtga ttggcatcac cgctgtattc
     2101 gccctttgtg ccgtggttta caccttcttc gccacgccga tttatagtgc cgacgcactg
     2161 gtacaaatcg agcaaagcag cggcaattcg ttagtgcagg atatcggatc ggcgttagcc
     2221 aacaaaccgc ctgcatcgga cgccgagatc cagttgattc gttcgcgcct ggtgcttggt
     2281 aaaacggtgg atgatctcga cctcgatatt gcggtgagca aaaacacgtt cccgattttc
     2341 ggtgcgggct gggatcgcct gatgggacgc cagaacgaga cggtgaaagt gactaccttt
     2401 aaccgtccga aagagatgga ggatcaggtg tttacgctta atgtgctgga caacaaaaac
     2461 tacaccctga gcagcgatgg cggctttagc gcccgtgggc aagcgggcca gatactgaaa
     2521 aaagaaggcg tcacgctgat ggttgaagcc attcacgccc gcccgggcag tgagtttacc
     2581 gtcaccaaat actccacgct ggggatgatc aatcaactgc aaaacagcct gacggtaacg
     2641 gagaacggca aagacgcagg cgtactgagc ctgacttata ccggtgaaga tcgcgaacag
     2701 atccgcgaca ttcttaacag catcgcccgt aactatcagg aacaaaatat tgagcgcaaa
     2761 tcggcggaag cgtcgaaaag cctcgctttc ctcgcgcaac agttaccgga agtacgtagc
     2821 cgccttgatg ttgccgaaaa caaactgaat gccttccgtc aggataaaga ttctgttgat
     2881 ctgccgctgg aagcgaaagc ggtgctcgat tcgatggtga acatcgacgc ccagttgaac
     2941 gaactgacct ttaaagaggc ggaaatctcc aagctgtaca ccaaagttca ccccgcgtac
     3001 cgcacgctgc tggagaaacg tcaggcgctg gaagacgaaa aagccaaact taatggtcgc
     3061 gtaacggcga tgccgaaaac ccagcaggaa attgtccgtc tgacccgcga tgtcgagtct
     3121 ggtcagcagg tctatatgca actgctgaat aaagagcagg agctgaaaat caccgaggcc
     3181 agcaccgtcg gcgatgtgcg cattgttgac ccggcaatca ctcagcctgg tgtgctaaaa
     3241 ccgaagaaag ggctgattat ccttggggcg attatccttg gcctgatgct ctctatcgtg
     3301 ggggtgctgc tgcgctcgtt gtttaatcgc ggcatcgaaa gcccgcaggt gctggaagaa
     3361 cacggtatca gcgtctatgc cagcatcccg ctgtcggagt ggcagaaagc gcgcgatagc
     3421 gtcaaaacca tcaaagggat taaacgctat aaacagagcc agctactggc ggtggggaat
     3481 ccaaccgatc tggcgattga agccatccgc agccttcgta ccagtttgca cttcgcgatg
     3541 atgcaggcgc agaacaatgt gttgatgatg accggggtta gcccgtcaat cggtaaaacc
     3601 tttgtctgcg ccaacctggc ggcggtgatc agccagacca ataaacgcgt gttgttgatc
     3661 gactgcgata tgcgcaaagg ctacacccat gagctgttgg gcactaataa cgttaatggc
     3721 ctgtcggaaa ttctgattgg tcagggcgat attactacag ctgctaaacc gacctctatt
     3781 gccaaatttg acctgatccc gcgcggtcag gtaccgccaa atccttctga actgttgatg
     3841 agcgaacgct ttgccgaact ggtgaactgg gcgagtaaaa actacgacct ggtgttgatt
     3901 gatacgccgc cgattctggc agtgaccgat gcggcaattg ttggtcgtca tgtcggaacc
     3961 acgttaatgg tggcgcgtta tgcggtcaac acattgaaag aagtggaaac cagtctgagc
     4021 cgctttgagc aaaacggtat tccggtgaaa ggggtgattc tgaactccat cttccgccgc
     4081 gccagcgcgt atcaggatta tggctattac gaatacgaat ataagtcgga tgcgaaataa
     4141 cgaggcctgc attcgcaccg ccccgtaggc cggataaggc gctcacgccg catccggcaa
     4201 gcaaaccagc tcataagccg ggagtacaac ctatgaaaga caatccgctg atctcaatct
     4261 atatgccgac ctggaaccgc caacaactgg cgattcgggc gataaaatcg gtcttgcgcc
     4321 aggactacag caactgggag atgatcatcg tggatgattg ctccacctcc tgggaacaac
     4381 tgcaacagta cgtcaccgcc ctcaacgatc cgcgtattac ctacattcac aacgacatta
     4441 acagcggggc gtgcgcggta cgtaaccagg cgattatgct ggcgcaaggg gaatacatta
     4501 ccgggatcga tgacgatgat gaatggacgc ccaaccgcct gagcgtcttc ctcgcccata
     4561 aacagcaact ggtcactcac gcctttttgt acgctaacga ttacgtttgc cagggcgaag
     4621 tttattccca accggcaagc ttaccactgt atcccaaatc gccgtactca cgccgcctgt
     4681 tctataaacg caatatcatt ggcaatcagg tctttacctg ggcatggcgt ttcaaagagt
     4741 gtttgttcga taccgaactg aaagcggcac aggattacga catcttcctg cggatggtgg
     4801 tggagtatgg cgaaccgtgg aaagtggaag aggcgacgca gatcctgcat atcaatcacg
     4861 gtgaaatgca gatcacctcg tcaccgaaaa aattctccgg atacttccac ttttaccgca
     4921 agcacaaaga caaattcgac cgtgccagta aaaaatatca gctgtttacc ctctaccaga
     4981 tccgcaataa acgtatgacc tggcgcactt tgctgacgct gctgtcggtg cgtaacggca
     5041 aacgtctggc tgatggtatt cgggggcgct aaatatgctg gaagatctac gcgccaacag
     5101 ctggagttta cgcccgtgct gcatggttct tgcctatcgc gtcgctcatt tttgttcggt
     5161 gtggcgcaaa aagaacgttc tcaacaatct ttgggcggcc ccgctgctgg tgctgtatcg
     5221 cattatcacc gaatgctttt tcggttatga aatccaggct gccgcgacca ttggccgccg
     5281 ctttactatc catcacggtt acgccgtagt gatcaataaa aacgtggtag cgggggatga
     5341 tttcaccatt cgccacggcg tcactatcgg caatcgtggt gccgataaca tggcatgtcc
     5401 acacattggc aacggcgtcg aactcggtgc caacgtcatt attcttggtg atatcacgct
     5461 tggtaacaac gtcaccgtgg gcgcgggcag cgtggtgctc gactctgtcc cggacaacgc
     5521 gctggtggtg ggagaaaaag cgcgagtgaa ggtaattaaa tgaatattct gcaatttaat
     5581 gtgcgactgg cggaaggcgg ggcggcaggt gtggcgttag atctccacca gcgtgcgctg
     5641 caacaggggc tggcgtcaca ttttgtctac ggctacggca aaggcggcaa agagagcgtc
     5701 agccatcaga actatccgca ggtcatcaaa catacgccgc ggatgaccgc aatggcgaat
     5761 attgccctgt ttcgtctgtt taatcgcgat ctgtttggca atttcaatga gttatatcgc
     5821 accattactc gtacaccggg tccggtggtc ctgcattttc atgtgctgca cagctactgg
     5881 ctaaatctta agagcgtggt gcgcttttgc gaaaaggtga aaaaccacaa accggatgtc
     5941 actctggtct ggacgctgca cgaccactgg agcgttaccg ggcgctgcgc ctttaccgac
     6001 ggttgtgaag gctggaaaac gggctgccag aaatgcccga ccttaaataa ctatccgccg
     6061 gtgaagattg atcgggcgca ccagctggtg gcgggcaaac gccagttatt ccgtgagatg
     6121 ctggcgctgg gctgtcagtt tatttccccc agccagcatg tggctgacgc tttcaatagc
     6181 ctgtacggtc cagggcgttg ccggattatc aataacggta ttgatatggc aaccgaggcg
     6241 attctggcgg atttgcctcc ggtgcgcgaa actcagggca agccgaaaat cgcggtggta
     6301 gcgcatgacc tgcgttacga cggcaaaact aaccagcaac tggtacgcga gatgatggcg
     6361 ctgggcgaca aaatcgaact gcataccttt ggtaagttct cgccgttcac cgctggcaac
     6421 gtggttaatc acggctttga aactgacaag cgcaagttga tgagcgcgct caatcagatg
     6481 gatgcgctgg tgttcagttc tcgcgtcgat aactacccgc tgattttgtg tgaggcgtta
     6541 tcaattggcg tgccggtgat tgccacccat agcgacgcgg cgcgtgaagt gttgcaaaaa
     6601 tccggcggta aaaccgtcag cgaagaagag gtgctgcaac tggtccagtt aagcaaaccg
     6661 gaaatcgcgc aggcgttatt tggtaccacg ctggctgagt tcagccaacg cagccgcgcc
     6721 gcctacagtg gacaacagat gctggaggag tatgtcaact tctatcagaa tctgtagcta
     6781 cctgctgctg ccgctgatct acctgctggt taacgtcaaa atcacccagc ttggcgaaag
     6841 tttccccatc accatcgtca cttttttacc tgtcttgtta ctgctgtttt tagaacgcat
     6901 cagcgttaaa aaattgatga ttgccttagg gatttgcgcg ggactcacgg cgtttaacta
     6961 cctgtttggt cagtcgctgg atgccagtaa atacgtcact tcaactatgc tgtttgtcta
     7021 tatcgtgatc attattggta tggtgtggag tattcgcttt aaaacgattt cgccacacaa
     7081 ccatcgcaag atattacgtt tcttttatct ggtggtcggg ctggtggtgg cgctggcggc
     7141 ggtggagatg gcgcaaatta tcctcaccgg cggcagcagt attatggagt cgatttcgaa
     7201 atatctgatt tacagcaaca gctatgtgct gaatttcatc aaattcggcg gcaagcgcac
     7261 gacagcactt tatttcgaac cggcattttt cgctctggca ttaatctcaa tttggctcag
     7321 catcaaacag tttggtatca aaacgcctaa aacagatgct atgattctcg cagggataat
     7381 attatccgga tcgttttcag gggttatgac ctttatcctg ttttatttgc tggagtgggc
     7441 atttcaatat ctgaataaag aggcgattaa gaaaaagtta ccgttagcat tgatttctct
     7501 ggctgtattc ctggttggtg tggtaattgc atttccttat atttccaccc gtctgggcga
     7561 tttaggtacg gaaggatcgt catcgtatta tcgtattgtc ggtccgctgg tgatggtcgg
     7621 ttattctttg acccatattg acggtgtagt cagatttggc tcactttatg aatatgtcgc
     7681 atcattcgga atatttaacg gtgcggatgt cggaaaaacc atagacaatg gtttgtatct
     7741 gctgattatt tatttttctt ggttcgcggt gtttttatcg ctgtggtaca tggggaaagt
     7801 gataaaaatg atgatcaacg cttttggtga taaccgcaat tttcgcgtgc aattatatct
     7861 ttttactccg gtatcgctgt tttttaccgg ttcgatattt agcccggaat atgcattttt
     7921 aatcgtctgt ccgtttattt tgcgaaaagc gttaaatatt acgaggtaag aataagaaca
     7981 tgttgcttag cataatcact gtcgcgtttc gtaacctcga agggatagtc aaaacacatg
     8041 cctcgctggc gcatctggcg caggcggaag atatcagctt cgaatggatt gttgtcgatg
     8101 gcggttccaa tgacggcact cgtgagtacc tggaaaatct caatggtatc tataacctac
     8161 gctttgtcag cgagccagat aacggtatct acgacgccat gaacaagggc attgcgatgg
     8221 cacaaggcaa gttcgcgttg tttctcaact cgggcgatat ttttcatcag gatgccgcat
     8281 attttgtccg taagttaaaa atgcaaaaag ataacgtgat gatcaccggc gatgcgctgc
     8341 tggattttgg cgacgggcat aaaattaaac gtagcgccaa accgggctgg tatatttatc
     8401 acagcctgcc cgccagtcat caggcgatat ttttcccggt atccggtttg aaaaaatggc
     8461 gttatgacct ggaatataaa gtttcttctg attacgcgct ggcagccaaa atgtataaag
     8521 caggttatgc atttaaaaaa ctcaatggcc tggtgtctga attttctatg ggtggggtat
     8581 ctaccaccaa taatatggaa ttgtgtgctg acgcgaaaaa agtccaacgg caaatattac
     8641 atgtgcctgg cttttgggct gaattatcct ggcatttacg ccagcgtact acctcaaaga
     8701 cgaaagcctt atataacaaa agctgaatat aagggaaaac gatgcaggat ttaagcggtt
     8761 tctcggtgcc gaaagggttc cggggcggca acgctattaa agtgcaattg tggtgggcag
     8821 tacaggcaac aatatttgcc tggtcgccac aagtattgta tcgctggcgg gcatttttat
     8881 tacgtttatt cggcgcaaaa ataggaaaaa acgtagttat tcgtccgtca gtaaaaatta
     8941 cctatccgtg gaaattaacc ttaggtgatt acgtgtgggt cggcgatgac gtcaatttat
     9001 ataccctcgg tgaaataacc attggcgcac attcggtgat atcgcaaaaa agttatttat
     9061 gcaccgggag ccacgaccat gcaagtcaac atttcaccat taacgccacg cctattgtga
     9121 ttggcgagaa atgctggctg gcaaccgatg tttttgttgc cccaggcgtc acgattggcg
     9181 acggcaccgt cgtgggtgcg cgaagcagtg tttttaaatc gcttccggca aatgtggttt
     9241 gccgggggaa tcccgcagtg gtgatacgca aacgcgttga aactgaataa attcaaaaaa
     9301 aatacagagg aataagacat gtcaaaagtc gctctcatca ccggtgtaac cggacaagac
     9361 ggttcttacc tggcagagtt tctgctggaa aaaggttacg aggtgcatgg tattaagcgt
     9421 cgcgcatcgt cattcaacac cgagcgcgtg gatcacattt atcaggatcc gcacacctgc
     9481 aacccgaaat tccatctgca ttatggcgac ctgagtgata cctccaacct gacgcgtatt
     9541 ttgcgtgaag tgcagccgga tgaagtgtac aacctgggcg caatgagcca cgttgcggtt
     9601 tcttttgagt caccggaata taccgctgac gtcgacgcga tgggtacgct gcgcctgctg
     9661 gaggcgatcc gcttcctcgg tctggaaaag aaaacccgtt tctatcaggc ttccacctct
     9721 gaactgtatg gtctggtgca ggaaattccg cagaaagaga ccacgccgtt ctatccgcgc
     9781 tctccgtatg cggtcgccaa actgtatgcc tactggatca ccgtcaacta ccgtgaatcc
     9841 tacggcatgt atgcctgtaa cggtattctc ttcaaccatg aatccccgcg ccgcggcgaa
     9901 accttcgtta cccgcaaaat cacccgcgca atcgccaaca tcgcccaggg gctggagtcg
     9961 tgcctgtacc tcggcaatat ggattccctg cgtgactggg gccatgccaa agactacgta
    10021 aaaatgcagt ggatgatgct gcaacaggaa cagccagaag atttcgttat cgcgaccggc
    10081 gttcagtact ccgtacgtca gttcgtagaa atggcggcag cacagctggg catcaaactg
    10141 cgctttgaag gcacgggcgt tgaagagaag ggcattgtgg tttccgtcac cgggcatgac
    10201 gcgccgggtg ttaaaccggg tgatgtgatt atcgccgttg atccgcgtta cttccgtccg
    10261 gctgaagttg aaacgctgct cggcgacccg accaaagcgc acgaaaaact gggctggaaa
    10321 ccggaaatca ccctcagaga gatggtgtct gaaatggtgg ctaatgacct cgaagcggcg
    10381 aaaaaacact ctctgctgaa atctcacggc tacgacgtgg cgatcgcgct ggagtcataa
    10441 gtatgagtag acaacgcatt tttattgctg gccatcgcgg gatggtcggt tccgccatca
    10501 ggcggcagct cgaacagcgc ggtgatgtgg aacaggtatt acgcacccgc gacgagctga
    10561 acctgctgga cagccgcgcc gtgcatgatt tctttgccag cgaacgtatt gaccaggtct
    10621 atctggcggc cgcgaaagtg ggcggcattg ttgccaacaa cacgtatccg gcggatttca
    10681 tctatcagaa catgatgatt gagagcaaca tcattcacgc cgcgcatcag aacgacgtga
    10741 acaaactgct gtttctcgga tcgtcctgca tctacccgaa actggcaaaa cagccgatgg
    10801 cagaaagcga gttattgcag ggcacgctgg agccgaccaa cgagccttac gccattgcca
    10861 aaatcgccgg gatcaaactg tgcgaatcat acaaccgcca gtacggacgc gattaccgct
    10921 cagtcatgcc gaccaacctg tatggcccgc atgacaactt ccacccgagt aattcgcatg
    10981 tgatccctgc attgctgcgt cgcttccacg aggcgacggc acagaatgca ccggacgtgg
    11041 tggtgtgggg cagcggtacg ccgatgcgag aatttctgca cgtcgatgat atggcggcgg
    11101 cgagcattca tgtcatggag ctggcgcatg aagtctggct ggagaacacc cagccgatgc
    11161 tgtcgcacat taacgtcggc acgggcgttg actgcactat ccgcgagctg gcgcaaacca
    11221 tcgccaaagt ggtgggttac aaaggtcggg tggtttttga tgccagcaaa ccggatggta
    11281 cgccgcgcaa actgctggat gtgacgcgcc tgcatcagct tggctggtat cacgaaatct
    11341 cactggaagc ggggcttgcc agcacttacc agtggttcct tgagaatcaa gaccgctttc
    11401 gggggtaatg atgtttttac gtcaggaaga ctttgccacg gtagtgcgct ccacaccgct
    11461 tgtctctctc gactttattg tcgagaacag tcgcggcgag tttctgcttg gcaaaagaac
    11521 caaccgcccg gcgcagggtt actggtttgt gccgggaggg cgcgtgcaga aagacgaaac
    11581 gctggaagcc gcatttgagc ggctgacgat ggcggaactg gggctgcgtc tgccgataac
    11641 agcaggccag ttttacggtg tctggcagca cttttatgac gataacttct ccggcacgga
    11701 tttcaccact cactatgtgg tgctcggttt tcgcttcaga gtagcggaag aagaactgtt
    11761 actgccggat gagcagcatg acgattaccg ctggctgacg ccggacgcgc tgctcgccag
    11821 taacgatgtt cacgctaaca gccgcgccta ttttctcgct gagaagcgtg ccggagtacc
    11881 cggattatga aaatactggt ctacggcatt aactactcgc cggagttaac cggcatcggc
    11941 aaatacaccg gcgagatggt ggaatggctg gcggcgcaag gtcatgaggt gcgggttatt
    12001 accgcaccgc cttactaccc gcagtggcaa gtgggcgaga actattccgc ctggcggtac
    12061 aaacgagaag agggggccgc tacggtgtgg cgctgcccgc tgtacgtgcc aaaacagccg
    12121 agcaccctga aacgcctgtt gcatctcggc agttttgccg tcagcagttt tttcccgttg
    12181 atggcgcaac gtcgctggaa gccggatcgc attatcggcg tagtgccaac gctgttttgc
    12241 acgccgggaa tgcgcctgct ggcgaaactc tctggcgcgc gtaccgtgct gcatattcag
    12301 gattacgaag tagatgccat gctggggctg ggccttgccg gaaaaggcaa aggcggcaaa
    12361 gtggcacagc tggcaacggc gttcgaacgt agcggactgc ataacgtcga taacgtttcc
    12421 acgatttcgc gttcgatgat gaataaagcc atcgaaaaag gcgtggcggc ggaaaacgtc
    12481 atcttcttcc ccaactggtc ggaaatcgcc cgttttcagc atgttgcaga cgccgatgtt
    12541 gatgcccttc gtaaccagct tggcctgccg gataacaaaa aaatcattct ttactccggc
    12601 aatattggtg aaaagcaggg gctggaaaac gttattgaag cagccgatcg cctgcgcgat
    12661 gaaccgctga tttttgccat tgtcgggcag ggcggcggca aagcgcggct ggaaaaaatg
    12721 gcgcagcagc gtggactgcg caacatgcaa tttttcccgc tgcaatcgta tgacgcttta
    12781 cccgcactgc tgaagatggg cgattgccat ctggtggtgc aaaaacgcgg cgcggcagat
    12841 gccgtattgc cgtcgaaact gaccaatatt ctggcagtag gcggtaacgc ggtgattact
    12901 gctgaagccc acacagaact gggacagctt tgcgaaacct ttccgggcat tgcggtttgc
    12961 gtagaaccgg aatcggttga ggcgctggtg gcggggattc gtcaggcgct cctgctgccc
    13021 aaacacaaca cggtggcacg tgaatatgcc gaacgcacgc tcgataaaga gaacgtgtta
    13081 cgtcaattta taaatgatat tcggggataa ttatggcgca gtcgaaactc tatccagttg
    13141 tgatggcagg tggctccggt agccgcttat ggccgctttc ccgcgtactt tatcccaagc
    13201 agtttttatg cctgaaaggc gatctcacca tgctgcaaac caccatctgc cgcctgaacg
    13261 gcgtggagtg cgaaagccct gtggtgattt gcaatgagca gcaccgcttt attgttgcgg
    13321 aacaactgcg tcaactgaac aaactcactg agaacattat tctcgaaccg gcagggcgta
    13381 acacagcacc ggccattgcg ctggcggcgc tggcggcaaa acgtcatagc ccggagaacg
    13441 acccattaat gctggtgctg gcggcggatc atgtgattgc cgatgaagac gcattccgcg
    13501 ccgccgtgcg taacgctatg ccgtatgccg aagcgggcaa gctggtgacc ttcggcattg
    13561 tgccggatct acctgaaacc ggatatggct atattcgtcg cggtgaagtg tcggcgggtg
    13621 agcaggatac ggtggccttt gaagtggcgc agtttgtcga aaaaccaaat ctggaaaccg
    13681 cgcaggccta tgtggcaagc ggcgaatatt actggaacag cggtatgttc ctgttccgtg
    13741 ccggacgcta tctcgaagaa ctgaaaaaat atcgcccgga tattctcgac gcctgtgaaa
    13801 aagcgatgag cgccgtcgat ccggatctcg attttattcg cgtggatgaa gacgcgtttc
    13861 tggcctgccc ggaagagtcg gtggattacg cggtcatgga acgtacggca gatgccgttg
    13921 tggtgccgat ggatgcgggc tggagcgatg tcggctcctg gtcctcatta tgggagatca
    13981 gcgcccacac cgccgagggc aacgtttgcc acggcgatgt gattaatcac aaaactgaaa
    14041 acagctatgt gtatgccgaa tctggcctgg tcaccaccgt cggggtgaaa gatttggtgg
    14101 tagtgcagac caaagatgcg gtgctgattg ccgaccgtaa cgcggtgcag gatgtgaaaa
    14161 aagtggtcga gcagatcaaa gccgatggtc gccatgagca tcgggtgcat cgcgaagtct
    14221 atcgcccgtg gggcaaatat gactctatcg acgcgggcga ccgctaccag gtgaaacgca
    14281 tcaccgtgaa accgggcgaa ggcttgtcgg tacagatgca ccatcaccgc gcggaacact
    14341 gggtggttgt cgcgggaacg gcaaaagtca ccattgacgg tgatatcaaa ctgcttggtg
    14401 aaaacgagtc gatttatatt ccgctggggg cgacgcactg cctggaaaac ccggggaaaa
    14461 ttccgctcga tttaattgaa gtgcgctccg gctcttatct cgaagaggat gatgtggtgc
    14521 gcttcgcgga tcgctacgga cgggtgtaaa cgtcgcatca ggcgctgatt gtcggatgcg
    14581 gcgtgaacgc cttatccgac ctacggttcg gttttgtagg cctgataaga cgcggcagcg
    14641 tcgcatcagg caatgaatgc gaaaccgcgg tgtaaataac gacaaaaaca aaattggccg
    14701 tttcggtcag ggccaactat tgcctgaaaa agggtaacga tatgaaaaaa ttaacctgct
    14761 ttaaagccta tgatattcgc gggaaattag gcgaagaact gaatgaagat attgcctggc
    14821 gcattggtcg tgcctatggc gaatttctca aaccgaaaac cattgtgtta ggcggtgatg
    14881 cccgtctcac cagcgaaacc ttaaaactgg cgctggcaaa aggtttacag gatgcgggcg
    14941 ttgacgtgct ggatattggt atgtccggca ccgaagagat ctatttcgcc acgttccatc
    15001 tcggcgtgga tggcggcatt gaagttactg ccagccataa cccgatggat tacaacggca
    15061 tgaaactggt gcgcgaaggg gctcgcccga tcagcggcga taccggactg cgcgatgtcc
    15121 agcgtctggc agaagccaac gactttcctc ccgttgatga aaccaaacgc ggtcgctatc
    15181 agcaaatcaa tctgcgtgac gcttacgttg atcacctgtt cggttatatc aacgtcaaaa
    15241 accttacgcc gctcaagctg gtgatcaact ccgggaacgg cgcggcgggt ccggtggtgg
    15301 acgccatcga agcccgcttt aaagccctcg gcgcaccggt ggaattgatc aaagtgcaca
    15361 acacgcccga cggcaatttc cccaacggta ttcctaaccc gctgctgccg gaatgtcgcg
    15421 acgacacccg caatgcggtc attaaacacg gcgcggatat gggcattgct tttgatggcg
    15481 attttgaccg ctgtttcctg tttgacgaaa aagggcagtt tatcgagggc tactacattg
    15541 tcggtctgct ggcagaagcg ttcctcgaaa aaaatcccgg cgcgaagatc atccacgatc
    15601 cacgtctctc ttggaatacc gttgatgtgg tgactgccgc aggcggcacc ccggtaatgt
    15661 cgaaaaccgg acacgccttt attaaagaac ggatgcgcaa ggaagacgcc atctacggtg
    15721 gcgaaatgag cgcccaccat tacttccgtg attttgctta ctgcgacagc ggcatgatcc
    15781 cgtggctgct ggtcgccgaa ctggtgtgcc tgaaaggaaa gacgctgggc gaactggtgc
    15841 gcgaccggat ggcagcgttt ccgtcaagcg gtgagatcaa cagcaaactg gcgcaacccg
    15901 ttgaggcgat taaccgcgtg gaacagcatt ttagccgtga ggcgctggcg gtggatcgca
    15961 ccgatggcat cagcatgacc tttgccgact ggcgctttaa tctgcgctcc tccaacaccg
    16021 aaccggtggt gcggttgaat gtggaatcac gcggtgatgt gccgctgatg gaagcgcgaa
    16081 cgcgaactct gctgacgttg ctgaacgagt aatgtcggat cttcccttac cccattgcgg
    16141 gtaaggggct aataacagga acaacgatga caaatctaaa aaagcgcgag cgagcgaaaa
    16201 ccaatgcatc gttaatctct atggtgcaac gcttttcaga tatcaccatc atgtttgccg
    16261 gactatggct ggtttgcgaa gtgagcggac tgtcattcct ctacatgcac ctgttggtgg
    16321 cgctgattac gctggtggtg ttccagatgc tgggcggcat caccgatttt tatcgctcat
    16381 ggcgcggtgt tcgggcagcg acagaatttg ccctgttgct acaaaactgg accttaagcg
    16441 tgattttcag cgccggactg gtggcgttca acaatgattt cgacacgcaa ctgaaaatct
    16501 ggctggcgtg gtatgggctg accagtatcg gactggtggt ttgccgttca tgtattcgca
    16561 ttggggcggg ctggctgcgt aatcatggct ataacaagcg catggtcgcg gtggcggggg
    16621 atttagccgc cgggcaaatg ctgatggaga gcttccgtaa ccagccgtgg ttagggtttg
    16681 aagtggtggg cgtttaccac gacccgaaac tgggcggcgt ttctaacgac tgggcgggta
    16741 acctgcaaca gctggtcgag gatgctaaag caggcaagat tcataacgtc tatatcgcga
    16801 tgcaaatgtg cgacggcgcg cgagtgaaaa aactggtcca tcaactggcg gacaccacct
    16861 gttcggtgct gctgatcccc gatgtcttta ccttcaacat tctccattca cgtcttgaag
    16921 agatgaacgg cgttccggtg gtgccgcttt acgacacgcc gctttccggg gttaaccgcc
    16981 tgctcaaacg tgcggaagac attgtgctgg cgacgcttat cctgctgctg atctccccgg
    17041 tgctgtgctg cattgcgctg gcggtgaaac tcagttcacc tgggccggtt attttccgcc
    17101 agactcgcta cggcatggat ggcaagccga tcaaagtgtg gaagttccgt tccatgaaag
    17161 tgatggagaa cgacaaagtg gtgacccagg cgacgcagaa cgatccgcgc gtcaccaaag
    17221 tggggaactt tctgcgccgc acctcgctgg atgaattgcc gcagtttatc aatgtgctga
    17281 ccggggggat gtcgattgtc ggtccacgtc cgcacgcggt ggcgcataac gaacagtatc
    17341 gacagctcat tgaaggctac atgctgcgtc ataaggtgaa accgggcatt accggctggg
    17401 cgcagattaa cggctggcgc ggcgaaaccg acacgctgga gaaaatggaa aaacgcgtcg
    17461 agttcgacct tgagtacatc cgcgaatgga gcgtctggtt cgatatcaaa atcgttttcc
    17521 tgacggtatt caaaggattc gttaacaaag cggcatattg atatgagctt acgtgaaaaa
    17581 accatcagcg gcgcgaagtg gtcggcgatt gccacggtga tcatcatcgg cctcgggctg
    17641 gtgcagatga ccgtgctggc gcggattatc gacaaccacc agttcggttt gcttaccgtg
    17701 tcgctggtga tcatcgcgct ggcagatacg ctttctgact tcggtattgc taactcgatt
    17761 attcagcgaa aagaaatcag ccaccttgaa ctcaccacgc tgtactggct gaacgttggg
    17821 ctggggcttg tggtgtgtgt ggcggtgttt ttgttgagtg atctcattgg tgacgtgctg
    17881 aacaacccgg acctggcacc gttgattaaa acattgtcgc tggcgtttgt ggttatcccc
    17941 cacgggcaac agttccgcgc gttgatgcaa aaggagctgg agttcaacaa aatcggcatg
    18001 atcgaaacca gcgcggtact ggcgggcttc acttttacgg tggttagcgc ccatttctgg
    18061 ccgctggcga tgaccgcgat cctcggttat ctggtcaata gtgcggtgag aacgctgctg
    18121 tttggctact ttggccgcaa aatttatcgc cccggtctgc atttctcgct ggcgtctgtt
    18181 gcgccgaact tacgctttgg tgcctggctg acggcggaca gcatcatcaa ctatctcaat
    18241 accaaccttt caacgctggt gctggcgcgt attctcggcg cgggcgtggc agggggatac
    18301 aacctggcgt acaacgtggc cgttgtgccg ccgatgaagc tgaacccgat catcacccgt
    18361 gtgttgtttc cggcattcgc caaaattcag gacgataccg aaaagctgcg cgtcaacttc
    18421 tacaagctgc tgtcggtggt ggggattatc aactttccgg cgctgctcgg gctgatggtg
    18481 gtgtcaaata actttgtacc gctggtattt ggcgaaaagt ggaacagcat tattccggtg
    18541 ctgcaattgc tgtgtgtggt gggtctgctg cgctccgtag gtaacccgat tggttcgctg
    18601 ctgatggcga aagcgcgggt cgatatcagc tttaaattca acgtattcaa aacatttctg
    18661 tttattccgg cgattgttat tggtgggcag atggcgggcg caatcggcgt cacgctcggt
    18721 ttcctgctgg tgcagattat caacaccatt ctgagttact tcgtgatgat taaaccggtt
    18781 cttggttcca gttatcgcca gtacatcctg agtttgtggc tgccgtttta tctctcgctg
    18841 ccgacgctgg tggtcagtta tgtgctgggc attgtgctga aagggcaact ggcgctgggg
    18901 atgctgctgg cggtgcaaat agccgcgggc gtgctggcgt ttgtggtgat gattgtgctg
    18961 tcgcgccatc cgctggtggt ggaagtgaag cgccagtttt gtcgcagcga aaaaatgaaa
    19021 atgcttttac gggcggggtg agttgttgtt ttcggttccg acaacggttg ccgggtgcag
    19081 cgtaaaacac cttatccatc ctacggttct gtgctcgggt cttgtaggcc tgataagacg
    19141 cgccagcgtc gcatcaggca aaaaacggca acgaacacaa caaacacagc ctcacctggc
    19201 atcaaattta tgaggaaaaa atgaaattac ttattctggg caaccacact tgcggcaatc
    19261 gtggcgacag cgccatcttg cgcggcttac ttgatgccat caacatcctc aatccacata
    19321 ccgaagtgga cgtgatgagc cgctatccgg tcagttcttc ctggctgctc aaccgcccgg
    19381 taatgggtga tccgctgttc cttcaaatga aacaacacaa cagcgcggcg ggcgttgtcg
    19441 ggcgcgttaa aaaagtcctc cgtcgccgct atcagcacca ggtattgctc tcacgcgtca
    19501 ccgacactgg caagctgcgc aatatcgcca tcgcccaggg attcaccgac ttcgtgcgcc
    19561 tactgtcagg ttacgacgcc attattcagg tcggcggatc gttttttgtc gatctctacg
    19621 gcgtgccgca gtttgaacat gcactttgca catttatggc gaaaaagccg ctgtttatga
    19681 ttggtcacag cgtcggtccc ttccaggatg agcaatttaa ccaactggcg aactacgttt
    19741 ttggtcactg cgacgcgctg atcttgcgcg aatcggtcag cctcgatcta atgaaacgca
    19801 gcaatatcac cactgcaaaa gttgaacatg gcgtcgatac cgcgtggctg gtcgatcacc
    19861 acacagaaga cttcaccgcc agctatgccg tccaacactg gctggacgtt gccgcacaac
    19921 agaaaacggt ggcaattacc ctgcgcgaac tggcaccgtt cgacaaacgc ctcggcacca
    19981 ctcagcaagt gtatgaaaaa gcctttgccg gggtggtcaa tcgcattctc gacgaaggct
    20041 atcaggtcat tgcgctctcc acctgtacgg gtatcgacag ctataacaaa gacgaccgca
    20101 tggtggcgct caacctgcgc cagcacatca gcgatcctgc ccgttatcac gtagtgatgg
    20161 atgaacttaa cgatctggaa atgggcaaaa ttctcggtgc ctgtgaactc accgtcggta
    20221 cgcgcctgca ctccgccatt atctcaatga actttgctac tccggcgatt gccatcaact
    20281 atgaacataa atccgccggg gttatgcagc agctgggact accggagatg gcaattgata
    20341 tccgtcattt attagacggc agcctgcaag cgatggttgc ggatacctta ggccagcttc
    20401 cggcgctgaa cgcacgactt agcgaagccg tcagtcgtga gcgtcagacg ggaatgcaga
    20461 tggtgcagtc cgtactggaa cgcatcgggg aggtgaaatg aaggtcggct tcttcttatt
    20521 gaaatttccg ctgtcgtcag aaaccttcgt tcttaaccaa atcactgcgt ttattgatat
    20581 gggctttgaa gtggagattg tcgcgctgca aaaaggcgac acacaaaaca cccacgtggc
    20641 atggactaaa tacaaccttg ccgccagaac ccgctggtta caggacgaac ctacgggcaa
    20701 agtggcgaaa ctgcgccacc gagccagtca gaccttgcgc ggcattcatc gtaaaaatac
    20761 ctggcaggcg ctcaacctca aacgctatgg tgccgagtcg tggaacctga ttttgtctgc
    20821 catttgcggc caggtcgcaa caccgtttcg cgccgatgtg ttcatcgctc attttggccc
    20881 tgcgggggta accgcagcaa aactacgcga actgggtgtc attcacggca aaattgccac
    20941 tattttccac ggcattgata tttccagtcg ggaagtgctc aaccattaca ctcccgaata
    21001 tcaacaactg tttcgacgtg gcgacctgat gctaccgata agcgatctgt gggccggaag
    21061 gctgcaaaaa atgggctgcc cgagggaaaa aatcgccgta tcgcgcatgg gcgtagatat
    21121 gacgcgcttt agcccgcgtc ccgtgaaagc gcccgcaaca ccgctggaga ttatttccgt
    21181 cgcacgctta accgagaaaa aaggtctgca tgtggcgatt gaagcctgcc ggcagttgaa
    21241 agagcagggc gtggcatttc gctatcgcat cctcggcatt ggcccgtggg aacgacgcct
    21301 gcgcacgctc atcgaacaat atcaactgga agatgtggtg gagatgccgg gctttaaacc
    21361 gagccatgaa gtgaaagcga tgctcgacga cgcggatgtc ttcctgttgc catcggttac
    21421 aggtgcggat ggcgatatgg aaggcattcc ggtggcgctg atggaggcga tggcggtcgg
    21481 cattccggtg gtttctactc tgcatagcgg aataccggaa ctggtggagg ctgacaaatc
    21541 cggctggctg gtgcctgaga acgatgctcg cgcactggcg caacgactgg cggcgtttag
    21601 ccaactggac accgacgaat tggctccggt cgtcaaacgc gcgcgcgaaa aagttgaaca
    21661 cgattttaac cagcaggtga tcaatcgaga actcgccagc ttgctgcagg ctttatagag
    21721 gagaacgtat gccatttaaa aaactctccc gacgcacctt cctgacggca agctcggcgc
    21781 ttgccttcct tcatacccct ttcgctcgcg cacttcccgc ccgacaaagc gttaatatca
    21841 acgactacaa ccctcacgac tggatcgcct catttaaaca agccttcagc gaagggcaaa
    21901 cggttgtcgt gcctgctgga ttcgtttgtg acaatatcaa caccggcatc ttcattcctc
    21961 ctggcaaaac gttacacatc cttggaagcc tgcgcggcaa cggcagaggg cgatttgtct
    22021 tacaggacgg cagccaggtg acaggggagg agggcggcag tatgcataac atcaccctgg
    22081 atgtgcgtgg ttctgactgc accatcaaag ggctggcgat gagcggcttt ggtccggtaa
    22141 cgcagattta tatcggcggc aaaaacaaac gggtcatgcg caacctgacc atcgataacc
    22201 tgaccattag ccacgctaat tacgccatct tacgccaggg atttcataac cagattatcg
    22261 gtgccaacat caccaattgt aagttcagcg acttacaggg cgatgccatt gaatggaacg
    22321 tggcgattaa cgaccgcgat attttgatct ccgaccatgt catcgagcgc atcaactgta
    22381 ctaacggcaa aatcaactgg ggcattggca taggccttgc gggaagcact tacgataata
    22441 attacccgga agatcaggca gtgaaaaact ttgtcgtggc gaatatcacg ggatcggatt
    22501 gtcggcagtt gatacatgtt gaaaatggta aacattttgt tattcgtaat atcaaagccc
    22561 gcaatatcac gcaggatttc agtaagaaag caggtattga taacgccaca gtcgctattt
    22621 acggttgtga caatttcgtg attgataata ttgaaatgat taatagcgcc gggatgttaa
    22681 tcggctatgg ggtaattaaa ggcaattatc tctcgattcc gcaaaatttc cgagtgaata
    22741 atattcaact ggataacacc catcttgctt ataaattgcg cggcctccaa atctctgccg
    22801 ggaatgccgt ctcctttgtg gcactaacta acattgagat gaagcgtgcg tcgctggagt
    22861 tacacaacaa accgcaacat ctttttctgc gtaatatcaa agtgatgcag gaatcctctg
    22921 ttggacccgc attgattatg aacttcgaca tgcgcaaaga cgttcgaggc gtctttatgg
    22981 cgaaagaaga aacactgctg tctcttgcaa atgttcatgc ggtgaatgag aaaggacaaa
    23041 gctccgtcga tatcgacagg attaatcacc atattgttaa tgtggaaaag attaacttta
    23101 gattgccgga acggagagag tagatttgcg accattcata aacttactgg atataatgtt
    23161 ctaaaagatt ataatcctca cactaactgt taaagctggg gctgggcagc attgactact
    23221 cagggaaggt actcatcttt atcttgtgtt tcttacgtaa caaccagact aaagtgattt
    23281 atgaacgata acgttttgct cataggagct tccggattcg taggaacccg actacttgaa
    23341 acggcaattg ctgactttaa tatcaagaac ctggacaaac agcagagcca cttttatcca
    23401 gaaatcacac agattggtga tgttcgtgat caacaggcac tcgaccaggc gttagccggt
    23461 tttgacactg ttgtactact ggcagcggaa caccgcgatg acgtcagccc tacttctctc
    23521 tattatgatg tcaacgttca gggtacccgc aatgtgctgg cggccatgga aaaaaatggc
    23581 gttaaaaata tcatctttac cagttccgtt gctgtttatg gtttgaacaa acacaaccct
    23641 gacgaaaacc atccacacga ccctttcaac cactacggca aaagcaagtg gcaggcggag
    23701 gaagtgctgc gtgaatggta taacaaagca ccaacagaac gttcattaac tatcatccgt
    23761 cctaccgtta tcttcggtga acgcaaccgc ggtaacgtct ataacttgct gaaacagatc
    23821 gctggcggca agtttatgat ggtgggcgca gggactaact ataagtccat ggcttatgtt
    23881 ggaaacattg ttgagtttat caagtacaaa ctgaagaatg ttgccgcagg ttacgaggtt
    23941 tataactacg ttgataagcc agacctgaac atgaaccagt tggttgctga agttgaacaa
    24001 agcctgaaca aaaagatccc ttctatgcac ttgccttacc cactaggaat gctgggtgga
    24061 tattgctttg atatcctgag caaaattacg ggcaaaaaat acgctgtcag ctctgtgcgc
    24121 gtgaaaaaat tctgcgcaac aacacagttt gacgcaacga aagtgcattc ttcaggtttt
    24181 gtggcaccgt atacgctgtc gcaaggtctg gatcgaactc tgcagtatga attcgtccat
    24241 gccaaaaaag acgacataac gtttgtttct gagtaatgca gacgagtcag agaaaaaagg
    24301 accaggttgg actacgatta ttcggaaaga ggtactgatt aaattatcag tatgtggatt
    24361 tccgagtaat ccgagtaacc cagatgctga tggtgtgtag tgttatcgta aggtttaatc
    24421 aaagttatgc ggaactaagc tttggtatat agccataacg tttggctaac ctatggcaat
    24481 aatttacgca ctatcagcca cttagaggta ggaaaagaat gacgaattta aaagcagtta
    24541 taccggtagc aggtcttggg atgcatatgt tgcctgccac taaggcgatt cccaaagaga
    24601 tgctaccgat cgtcgacaag ccaatgattc agtacattgt tgacgagatt gtggctgcag
    24661 ggatcaaaga aatcctcctg gtaactcacg cgtccaagaa cgcagtcgaa aaccacttcg
    24721 acacctctta tgaattagaa tctctccttg aacagcgcgt gaagcgtcaa ctactggcgg
    24781 aagtacaatc tatctgtccg ccgggcgtga ccattatgaa cgtgcgtcag ggtgaacctt
    24841 taggtttagg ccactccatt ttatgtgcgc gacctgccat tggtgacaat ccatttgtcg
    24901 tggtgctgcc agacgttgtg atcgacgacg ccagcgccga cccgctacgc tacaaccttg
    24961 ctgccatgat tgcgcgcttc aatgaaacgg gacgcagcca ggtgctggca aaacgtatgc
    25021 cgggcgacct ctctgaatac tccgtcatcc agaccaaaga accgctggat cgtgaaggca
    25081 aagtcagccg cattgttgaa tttatcgaaa agccggatca gccgcagacg ctggactcag
    25141 acatcatggc cgttggtcga tatgtgcttt ctgccgatat ttggccggaa ctggaacgta
    25201 ctcagcctgg tgcatgggga cgtattcagc tgactgatgc cattgctgag ctggcaaaaa
    25261 aacaatccgt tgatgcaatg ctgatgactg gcgacagtta cgactgcggt aaaaaaatgg
    25321 gctatatgca ggcgtttgtg aagtatggac tacgcaactt gaaagaaggg gcgaagttcc
    25381 gcaaaggtat cgagaagctg ttaagcgaat aatgaaaatc tgaccgaatg taacggttga
    25441 taaggaaata atatcggcag tgaaaaacta tagatcaata aatatcactg ctgttgagct
    25501 tttataatta actatgctac caatgaataa atagtgtgca gatatgtatt ttttgtttgg
    25561 tagtcttgtt tctccctctg aataataaga taataaacgc ttggagatga tttttttaac
    25621 atggattatc atttcgtaca c
//
LOCUS       DAZKIP010000060        25325 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZKIP010000060 DAZKIP010000000
VERSION     DAZKIP010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.060"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            294..1118
                     /gene="bax"
                     /locus_tag="ACHOUK_002695"
     CDS             294..1118
                     /gene="bax"
                     /locus_tag="ACHOUK_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFP9898112.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTSRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            complement(1226..2404)
                     /gene="xylR"
                     /locus_tag="ACHOUK_002696"
     CDS             complement(1226..2404)
                     /gene="xylR"
                     /locus_tag="ACHOUK_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFP9898113.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(2482..3663)
                     /gene="xylH"
                     /locus_tag="ACHOUK_002697"
     CDS             complement(2482..3663)
                     /gene="xylH"
                     /locus_tag="ACHOUK_002697"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFP9898114.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            complement(3641..5182)
                     /gene="xylG"
                     /locus_tag="ACHOUK_002698"
     CDS             complement(3641..5182)
                     /gene="xylG"
                     /locus_tag="ACHOUK_002698"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFP9898115.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            complement(5260..6252)
                     /gene="xylF"
                     /locus_tag="ACHOUK_002699"
     CDS             complement(5260..6252)
                     /gene="xylF"
                     /locus_tag="ACHOUK_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9898116.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLQPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            6618..7940
                     /gene="xylA"
                     /locus_tag="ACHOUK_002700"
     CDS             6618..7940
                     /gene="xylA"
                     /locus_tag="ACHOUK_002700"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFP9898117.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAACMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            8012..9466
                     /gene="xylB"
                     /locus_tag="ACHOUK_002701"
     CDS             8012..9466
                     /gene="xylB"
                     /locus_tag="ACHOUK_002701"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFP9898118.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECALLEARVPLSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            9635..9976
                     /gene="yiaB"
                     /locus_tag="ACHOUK_002702"
     CDS             9635..9976
                     /gene="yiaB"
                     /locus_tag="ACHOUK_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFP9898119.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            10022..10459
                     /gene="yiaA"
                     /locus_tag="ACHOUK_002703"
     CDS             10022..10459
                     /gene="yiaA"
                     /locus_tag="ACHOUK_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFP9898120.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQRNIRDAGINPPKETQVTQEEYSE"
     gene            complement(10501..11496)
                     /gene="wecH"
                     /locus_tag="ACHOUK_002704"
     CDS             complement(10501..11496)
                     /gene="wecH"
                     /locus_tag="ACHOUK_002704"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFP9898121.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLFFYSAIALLYIALFTSINV
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRALGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYPYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            11671..11970
                     /gene="ysaB"
                     /locus_tag="ACHOUK_002705"
     CDS             11671..11970
                     /gene="ysaB"
                     /locus_tag="ACHOUK_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFP9898122.1"
                     /translation="MMMNSFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            12065..12976
                     /gene="glyQ"
                     /locus_tag="ACHOUK_002706"
     CDS             12065..12976
                     /gene="glyQ"
                     /locus_tag="ACHOUK_002706"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFP9898123.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            12986..15055
                     /gene="glyS"
                     /locus_tag="ACHOUK_002707"
     CDS             12986..15055
                     /gene="glyS"
                     /locus_tag="ACHOUK_002707"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFP9898124.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            15378..15530
                     /gene="hokA"
                     /locus_tag="ACHOUK_002708"
     CDS             15378..15530
                     /gene="hokA"
                     /locus_tag="ACHOUK_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFP9898125.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(15507..15578)
                     /locus_tag="ACHOUK_002709"
     CDS             complement(15507..15578)
                     /locus_tag="ACHOUK_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9898126.1"
                     /translation="MRNAVRKAGITFRRRLLLFQIAG"
     gene            complement(15718..15930)
                     /gene="cspA"
                     /locus_tag="ACHOUK_002710"
     CDS             complement(15718..15930)
                     /gene="cspA"
                     /locus_tag="ACHOUK_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFP9898127.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(16211..16501)
                     /gene="yiaG"
                     /locus_tag="ACHOUK_002711"
     CDS             complement(16211..16501)
                     /gene="yiaG"
                     /locus_tag="ACHOUK_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFP9898128.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            16935..17645
                     /gene="yiaF"
                     /locus_tag="ACHOUK_002712"
     CDS             16935..17645
                     /gene="yiaF"
                     /locus_tag="ACHOUK_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFP9898129.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(17695..18669)
                     /gene="ghrB"
                     /locus_tag="ACHOUK_002713"
     CDS             complement(17695..18669)
                     /gene="ghrB"
                     /locus_tag="ACHOUK_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFP9898130.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(18773..19432)
                     /gene="yiaD"
                     /locus_tag="ACHOUK_002714"
     CDS             complement(18773..19432)
                     /gene="yiaD"
                     /locus_tag="ACHOUK_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFP9898131.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            19585..21918
                     /gene="bisC"
                     /locus_tag="ACHOUK_002715"
     CDS             19585..21918
                     /gene="bisC"
                     /locus_tag="ACHOUK_002715"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFP9898132.1"
                     /translation="MTNSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVNAEKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRAAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFTEFWQANQL
                     IEMPENPDSERFIRFADFCRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLVP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGIQD
                     GDTVRLWNARGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(21887..22327)
                     /gene="yiaC"
                     /locus_tag="ACHOUK_002716"
     CDS             complement(21887..22327)
                     /gene="yiaC"
                     /locus_tag="ACHOUK_002716"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFP9898133.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKVNYWRECIPLVRDAY
                     LANAKNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYQAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(22324..22887)
                     /gene="tag"
                     /locus_tag="ACHOUK_002717"
     CDS             complement(22324..22887)
                     /gene="tag"
                     /locus_tag="ACHOUK_002717"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFP9898134.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDRKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            23045..23743
                     /gene="yhjY"
                     /locus_tag="ACHOUK_002718"
     CDS             23045..23743
                     /gene="yhjY"
                     /locus_tag="ACHOUK_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFP9898135.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            23972..25174
                     /gene="yhjX"
                     /locus_tag="ACHOUK_002719"
     CDS             23972..25174
                     /gene="yhjX"
                     /locus_tag="ACHOUK_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9898136.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTIASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVKFYVIFALLILSLALSTTIRQPEQ
                     KVLREAHI"
BASE COUNT         6301 a         6102 c         6410 g         6512 t
ORIGIN      
        1 ttcagacagt ttgccagcga taagccagac aaaactcatc cctgagcggt aagtccacag
       61 gatgagaagc aagggtgagc gatcgcgcgc aaaaaacggc tgaattttgc gataaacccc
      121 acattttctg cgatttagcg ccaatctgaa tcgttaactc gtgacatagt ttcagatttg
      181 gactattcct tggggtgctc ttgacagcta tttttgatat gatttgagat tcaactctca
      241 aatttgtgaa aaatataagg tgttggaatg attaaatccg accaggagac ctgatgattt
      301 tgactcccat acgacgatat ggggcgatga ttcttatgtt actcactctg gtgttttcga
      361 gtgaggtgtt agcgaagacg cacacaacaa cagcgagtca aaagtcccac ttaactaaag
      421 ctagtaataa acaggtaagc agtaaacaag agtattctcg caatagtgca aagagtagtt
      481 cacttcctga tttgcgaaaa tacccttccg gaacatcaag gaaaaaggcg tttctccgga
      541 ccgtaatgcc ttacattacc agccaaaatg cagccattac tgcagaacgt aactggctga
      601 tttcaaaaca gtatcagggc caatggtcac ctgctgagcg tgcgcgtctg aaagacatcg
      661 ccaaacgcta caaggtgaag tggtctggta atacgcgaaa aatcccgtgg aataccttgc
      721 ttgaacgcgt agacattatc cctaccagta tggtggcgac gatggctgca gcagaaagcg
      781 gttggggaac gtcgaagctg gcgcgcaaca acaacaatct tttcggcatg aaatgcatga
      841 aaggacgttg taccaatgcg ccaggtaaag tgaaagggta ctcacagttt agttctgtca
      901 aagaatcggt gagcgcctat gtcactaacc tcaatacgca tccggcttac tcttcgttcc
      961 gtaaatcgcg tgcgcagctg cgtaaagctg atcaggaagt gactgccaca gcgatgattc
     1021 acaagctgaa gggctactcg accaaaggga agagttataa caactacctg ttcgcaatgt
     1081 accaggataa ccaacggtta atcgcggcgc atatgtaatt gtatttcctc gagccttatc
     1141 cgacttgtcg gtcggataag gcttttgatc gcatcaggtg gttgtgctga gttacctgat
     1201 gcgacgccga caattctcat catcgctaca acatgacctc gctatttaca tcgcgatact
     1261 cttttggcgt cgtgtcatat gcttttttaa aaacagagta gaaatattgc agcgatggat
     1321 aaccgcacat ttgcgatatc tcattgatcg acaaggtggt tgaaatcagc agactgcgcg
     1381 ctttctccag cttctcagca tgaatcatgg catggatggt ttcacccacc tcttctttaa
     1441 aacgcttctc aagattggag cgcgagatcc cgaccgcatc gagtacctga tccactttaa
     1501 tccctttaca ggcgtgatta cgaatgtaat gcatggcctg aataacggcg ggatcggtca
     1561 gcgaacggta atctgttgag cgccgttcaa tgacgcgaac tggagggacc aaaatccgct
     1621 gtagcggcat ttcttcttta tctaataatc gatgcaacag ttttgccgcc tgatagccca
     1681 tttgccgcgc gccctgagcg accgaagaaa gggcgacacg cgacagatag cgggtcagtt
     1741 cttcgttatc gatgccaatc acgcataatt tttccggtac gggaatgtgt agatgttcac
     1801 atacttgcag aatatgccgc gcccgggcgt cagtaacggc aataatcccg gtttgcggtg
     1861 gcagtgtttg tagccagtct gccagccgat tttgcgcgtg ttgccagttc tctggtgcgg
     1921 tttctaaccc ctgataaacc actccgcgat acttttcttc ggcgacaagc tgacgaaatg
     1981 catattcgcg ttcagtggcc caacgtttgc cgcttgattc cggaagacca taaaaagcaa
     2041 agcggttaac gcctttctct tttaaatgca aaaatgcgct ttcaaccagc gcatagttat
     2101 cggtggcaat gtaatgaacg ggtgggtaac tttctgcaag gtgatacgag ccgccaaccc
     2161 caacaatggg gacgtcgaca tcagccagcg cttgctcgat ctgtttgtcg tcgaagtcgg
     2221 caatgacgcc atctcctaac cagtccttga ttttatcaat gcgggcgcgg aaatcttctt
     2281 caatgaaaat atcccattcc gattgtgacg cctgtaaata ttcccctacg ccttctacta
     2341 cctgccggtc ataggcttta ttggcattga acagtaatgt gatgcggtga cgtttagtaa
     2401 acatggttct tttcctgctg aatcatgcaa aaactcaaaa ccggtaatac gttaccggct
     2461 ttgagaaaat ttttatcaaa atcaagaacg gcgtttggtt gcggagtcca tccatactgc
     2521 cagcaacaga atcgcacctt taacgatata ctgccagaag gtcggtacat ccatcatact
     2581 cataccgtta tccagtgaag ccatgatgaa tgcccccatt accgctccag ccacgctccc
     2641 gacgccgcct gccagactgg tgccgccaat cacgcatgct gcaattgcgt ccagctcggc
     2701 gatattcccc gcagaaggtg aaccagcgcc aagtcgagaa ctgaggatta atccggcgat
     2761 ggctaccatt aatccgttaa tagcgaacac ggcaagtttg gtgcgttcaa cgttaattcc
     2821 ggagagacgt gctgcttcca gattgccgcc gatggcataa atgcgtcgtc caaatgctgt
     2881 ccgcgttgcc ataaacattc cgccgagtaa cagcaacgtc agcagcagaa caggagtagg
     2941 aacgccacgg taatcattca acagccagat tgcgcctaat acgatgatag cggttaaagc
     3001 ctggcgaccg actactgcgg tagaggctgg agactgcaaa cccaaagcct gacggcgcat
     3061 tcttccgcgc cattgccagc caacaaaagc cattaagcca agcgcgccaa tgatgaagcc
     3121 ggtgctggcg gggagatagc tttgcccaat ttgtgacatc gcggcgctgg tgggggatac
     3181 agtcgtgccg ttggtgatgc caatgagtat gccgcgaaat gccaacatgc ccgcgagagt
     3241 gacaataaat gaagggactt tgcggtacgc gacccaccat ccgttccagg caccgagaag
     3301 cagtcccaga accaacgtca caatgatggt aagtggcaaa ggccagccta gccagacgtc
     3361 acaaatcgcc gcgacgccac ctaatagccc catcattgag ccgacggaaa ggtcgatttc
     3421 agcagaaatt atgacgaaca ccattcctac cgcgaggatg ccggtaatcg cggtctggcg
     3481 taacaggttg gagacgttac gggcgcttaa gtaggcacca tcggtggtcc aggtaaagaa
     3541 cagcatgatt gcgatgatag ctgcaatcat cacgaagacc tgcaaattca gtgatttcag
     3601 cccggagaag ccaccggatg tcggtacggc caatttcact tcagacggat tgcttttcga
     3661 catgatgttc gctcctcaat gcggcttcca tcacctgctc ctgagtcagg ttatgattta
     3721 tcaggttggc ttttagtttc ccttcatgca ttactagtac acgatcgcta aggccgagca
     3781 cttcaggtaa ttcagaagag atgacaataa cggcaatacc ctgctggacg agttggttaa
     3841 ttaatttgta gatctcgtat ttcgcgccaa tatcgatacc cctggtgggt tcatcaagaa
     3901 tgagaatgcg cgggttaagt aacagacagc gagcgaggat cgctttttgc tgattgccgc
     3961 cgctcaaacg tccaatagca aggtcggggg acgacgtttt aactttgagt tgctggattg
     4021 attccagaat acatttttgc tctgccgcgt catcaagctg gctaatgcca ccggtaaatt
     4081 tattgagtgc ggcgagggta atatttttac caaccgccat taccggaacg atgccgtcgc
     4141 gctttctgtc ttcggggacc atcgcaatcc cctgggcgat ggcttgctga cagttacgaa
     4201 tatctacctg tttgccatca atataaattt ttccttccca ttgtccgggc cacacaccaa
     4261 acaggcactg aatggtctcg gtacgtccgg caccaacgag tccggcaata cccagtattt
     4321 cgccacgttt cagggaaaac gagacatcat taactcgttt aatatgacga ttaaccggat
     4381 gccatgccgt cagatgttca atacgtaata tttcatctcc ggtggtatgt ggttcattag
     4441 ggtaaagcgc ggttaactct cgcccgacca tcatggtgat aatatcgtct tcactcattc
     4501 cggcagcatc acgcgtacca atgtgctgtc cgtcgcgaat aacgcaaatc gtatcggaaa
     4561 tcgctttgac ttcgttgagt ttgtgcgaaa tataaataca ggcgataccg tgctgttgta
     4621 gatcgcgaat aatatccagt aaaaccgacg tttcctgctc agttaatgag gctgtcggtt
     4681 catcgagaat taacaagcgc acctgtttat taagtgcctt ggcaatttca accagttgtt
     4741 gttgcccaag ccctaaatcg ccaacgcggg tatcaggtga aatggataaa ctgacctgtg
     4801 cgagcagctt ctgacagcgt agcgtcatca ggtcataatc cataatgcca ttgtgggtta
     4861 tttcgttacc caggaagata ttttccagca cggtcaattc tttcaccagg gccaattcct
     4921 ggtgaatgat ggcgatacct ttgcgttcgg tatcgcggat gtgactcgcc tgaatctctt
     4981 ctcccgcaaa aataatttcg ccttcgtagg agccatgggg ataaatacca cacagcactt
     5041 tcatcagcgt tgatttacca gacccatttt ccccacagag tgagacgatt tcgccagcat
     5101 tcaaccgcaa gctgacgtta tcaatcgcct tcacactgcc gaaggttttg gtaatgttct
     5161 tcatttcaag tagataaggc atagcgactc cacctaagcc aattcattca cgcggcatgg
     5221 agagaaatca cgcccccgct ccgcgctggg gcgtaacgct tacagctcgc tctctttgtg
     5281 gaatccgtct ttaattaccg tatctttgat gttgttttta ttcacatcga tcggtgtcag
     5341 gaggcgggag gggacatctt tcaggccatt atttagtgaa gtatctgctt ttggctcctg
     5401 accattgccc aattcaacgg caatttctgc ggcagtattt gccagcaatg taataggttt
     5461 atataccgtc atagtttgcg taccggcagc aatacgttta atacctgcga gatccgcatc
     5521 ctggccagaa attgctactt tccctgataa accttgcgcg cttaatgcct gaattgcccc
     5581 acctgcggtg gcatcgtttg aggcaactac agcatcaatt ttgttattat tggcggttag
     5641 cgcgttttcc ataattttca atgcgttttc cggtaaccag ccatcaaccc attggtcacc
     5701 aacgacttta atttttccgg aatcaacgta aggttgtaac actttcattt gtccggcgcg
     5761 gaacagctta gcgttgttat ctaccggcga gccgcccatc aggaagtaat taccttgcgg
     5821 aacaatatcg accagggctt ttgcctgcag ttcaccgact ttttcgttat cgaaagaaat
     5881 ataaaaatcg atatccgcat cgttaatcat acggtcgtaa gctaatactt taatgccttc
     5941 ttgtttggct tcttttacaa cgttacttaa tacctgaccg ttatacggaa taatgacaag
     6001 aacatcgaca ccccggttta tcatgttttc aatctgcgac atttgtgttt cttcattgcc
     6061 atttgcagac tgtacaaata ctttcgcgcc gagagattct gcctttttca caaagatatc
     6121 tcgatctttt tgccagcgtt caagacggag atcatcaatc gccataccta ttttgacttc
     6181 tttggcgtgt gcagcaacgt tggtaagcag gagtgaggtg caaagggtga gtagaatgtt
     6241 ctttattttc atggtgtagg gccttctgta gttagaggac agctttaata agtaacaatc
     6301 accgcgataa acgtaaccaa tttttagcaa ctaaacaggg gaaaacaatt acagattttt
     6361 atctttcgat tacgattttt ggtttatttc ttgatttatg accgagatct tacttttgtt
     6421 gcgcaattgt acttattgca tttttctctt cgaggaatta cccagtttca tcattccatt
     6481 ttattttgcg agcgagcgca cacttgtgaa ttatctcaat agcagtgtga aataacataa
     6541 ttgagcaact gaaagggagt gcccaatatt acgacatcat ccatcacccg cggcattacc
     6601 tgattatgga gttcaatatg caagcctatt ttgaccagct cgatcgcgtt cgttatgaag
     6661 gctcaaaatc ctcaaacccg ttagcattcc gtcactacaa tcccgacgaa ctggtgttgg
     6721 gcaagcgtat ggaagagcac ttgcgatttg ccgcctgcta ctggcacacc ttctgctgga
     6781 acggggcgga tatgtttggt gtgggggcgt ttaatcgtcc gtggcagcag cctggtgagg
     6841 cactggcgtt ggcgaagcgt aaagcagatg tcgcatttga gtttttccac aagttacatg
     6901 tgccatttta ttgcttccac gatgtggatg tttctcctga gggcgcgtcg ttaaaagagt
     6961 acatcaataa ttttgcgcaa atggttgatg ttctggcagg caagcaagaa gagagcggcg
     7021 tgaagctgct gtggggaacc gctaactgct ttacaaaccc tcgctatggc gcgggtgcgg
     7081 cgacgaaccc agatccagaa gtcttcagct gggcggcaac gcaagttgtt acagcgatgg
     7141 aagcaaccca taaattgggt ggtgaaaact atgtcctgtg gggcggtcgt gaaggttacg
     7201 aaacgctgtt aaataccgat ttgcgtcagg agcgtgaaca actgggccgc tttatgcaaa
     7261 tggtggttga gcataaacat aaaatcggct tccagggcac gttgcttatc gaaccgaaac
     7321 cgcaagaacc gactaaacat caatatgatt acgatgctgc gacagtctat ggcttcctga
     7381 aacagtttgg tctggaaaaa gagattaaac tgaacattga agctaaccac gcgacgctgg
     7441 caggtcactc tttccatcat gaaatagcca ccgccattgc gcttggcctg ttcggttctg
     7501 tcgacgccaa ccgtggcgat gcgcaactgg gctgggacac cgaccagttc ccgaacagtg
     7561 tggaagagaa tgcgctggtg atgtatgaaa ttctcaaagc aggcggtttc accaccggtg
     7621 gtctgaactt cgatgccaaa gtacgtcgtc aaagtactga taaatatgat ctgttttacg
     7681 gtcatatcgg cgcgatggat acgatggcac tggcgctgaa aattgcagcg tgcatgattg
     7741 aagatggcga gctggataaa cgcatcgcgc agcgttattc cggctggaat agcgaattgg
     7801 gccagcaaat cctgaaaggc caaatgtcac tggcagattt agccaaatat gctcaggaac
     7861 ataatttgtc tccggtgcat cagagtggtc gccaggagca actggaaaat ctggtaaatc
     7921 attatctgtt cgacaaataa cggctaactg tgcagtccgt tggcccggtt atcggtagcg
     7981 ataccgggca tttttttaag gaacgatcga tatgtatatc gggatagatc ttggcacctc
     8041 gggcgtaaaa gttattttgc tcaacgagca gggtgaggtg gttgcttcgc aaacggaaaa
     8101 gctgaccgtt tcgcgcccgc atccactctg gtcggaacaa gacccggaac agtggtggca
     8161 ggcaactgat cgcgcaatga aagctctggg cgatcagcat tctctgcagg acgttaaagc
     8221 attgggtatt gccggccaga tgcacggagc aaccttactg gatgctcaac aacgggtatt
     8281 gcgccctgcc attttgtgga acgacgggcg ctgtgcgcaa gagtgcgctt tgctggaagc
     8341 gcgagttccg ctatcgcggg tgattaccgg caacctgatg atgcccggat ttactgcgcc
     8401 taaattgcta tgggttcagc ggcatgagcc ggagatattc cgtcaaatcg acaaagtatt
     8461 attaccgaaa gattacttgc gtctgcgtat gacgggggag tttgccagcg atatgtctga
     8521 cgcagctggc accatgtggc tggatgtcgc aaagcgtgac tggagtgacg tcatgctgca
     8581 ggcttgcgac ttatctcgtg accagatgcc cgcattatac gaaggcagcg aaattactgg
     8641 tgctttgtta cctgaagttg cgaaagcgtg gggtatggcg acggtgccag ttgtcgcagg
     8701 cggtggcgac aatgcagctg gtgcagttgg tgtgggaatg gttgatgcta atcaggcaat
     8761 gttatcgctg gggacgtcgg gggtctattt tgctgtcagc gaagggttct taagcaagcc
     8821 agaaagcgcc gtacacagct tttgccatgc gctaccgcag cgttggcatt taatgtctgt
     8881 aatgctgagt gcagcgtcgt gtctggattg ggccgcgaaa ttaaccggcc tgagcaatgt
     8941 cccagcttta atcgctgcag ctcaacaggc tgatgaaagt gccgagccag tttggtttct
     9001 gccttatctt tccggcgagc gtacaccaca caataatccc caggcgaaag gggttttctt
     9061 tggtttgact catcaacatg gccccaatga actggcgcga gcagtgctgg aaggcgtggg
     9121 ttatgcgctg gcagatggca tggatgtcgt gcatgcctgc ggcattaaac cgcaaagtgt
     9181 tacgttgatt ggtggcgggg cgcgtagtga gtactggcgt cagatgctgg cggatatcag
     9241 cggtcagcag ctcgattacc gtacgggggg ggatgtgggg ccagcactgg gcgcagcaag
     9301 gctggcgcag attgcggcga atccagagaa atcgctcatt gagttgttgc cgcaactacc
     9361 gttagaacag tcgcatctac cagatgcgca gcgttatgcc gcttatcagc cacgacgtga
     9421 aacattccgt cgcctctatc agcaacttct gccattaatg gcgtaaacgt tatcccctgc
     9481 ctgaccgggt gggggataat tcacatctat atatctcagc aattaattaa tatttagtat
     9541 gaatttattc tgaaaatcat ttgttaatgg catttttcag ttttgtcttt cgttggttac
     9601 tcgtaatgta tcgctggtag atatggagat cgttatgaaa acctcaaaaa ctgtggcaaa
     9661 actattattt gttgtcgggg cgctggttta tctggttggg ctatggatct catgcccatt
     9721 gttgagtgga aaaggctatt ttcttggcgt gttaatgaca gcaacttttg gcaactatgc
     9781 atatcttcgc gcagaaaaac tcgggcaact ggataatttt tttacccata tttgccagtt
     9841 agttgcgtta atcactatcg gtctcttgtt tatcggtgtt ttaaatgcac ctatcaatgc
     9901 ttatgaaatg gtgatctacc ccatcgcctt ttttgtctgc ttgtttggtc aaatgcgttt
     9961 gtttcgctcg gtatgagcaa cataaagctc ttacacattc aggaatgaaa ggaatactgt
    10021 gatggacaac aaaatatcaa cctattcacc ggcctttagt attgtgtcat ggatagctct
    10081 cgttggtggt atcgttacct atctgttagg gctatggaat gcagagatgc agttaaatga
    10141 aaaaggatat tattttgccg tactggtatt aggactgttt tctgcggcgt cttatcaaaa
    10201 gaccgttcgg gacaagtatg aaggcatacc gaccacttcc atttattata tgacctgcct
    10261 gactgtcttt attatctctg ttgcattact gatggtaggt ctgtggaatg cgacattatt
    10321 actcagcgaa aaaggttttt atggactggc tttcttctta agcttgtttg gtgcagtagc
    10381 ggtgcagaga aatattcgtg atgccggaat aaacccacca aaagaaacac aggttaccca
    10441 ggaagaatac agcgaataac tcacgtaagc ccggtcagtc caatgtgacc gggcttttac
    10501 ttaactcact aatctgtttc tgtcgattcg ttgtaccagc atagaaagta acaaactcgc
    10561 tgccaacgtc gcgcaaaaaa tccaaataat atccagtatt ggccaatttt taagctcaat
    10621 tccccgggtg cgcagcgcat ggataatcaa ggcgtggaat ccgtatatac ccaacgaatg
    10681 gcgggagatt aagccaagtc cgcgaatggt acgcgtatcc agcgtgtttt taaccagagt
    10741 caatagcgcg attgcgcaga taaaaaccat cggcccacag taaggatacc aggtatcggc
    10801 aaaatttccg cgccactgca attcatataa tgtcccgcga gagataataa aaactcccgt
    10861 cgcaaacagt gcggcgctca cccacgacag tgctttatgc tgtgtgtcca tcatccctaa
    10921 agcgcggccc aacatgccat acagaatgta gtaaaaagta tcgccattga tatataagtt
    10981 aattggcagc cattcaaaac cgtcgatttt ctgcggcacg gtgtttgggt tagcgatgat
    11041 accaatcacc accattagca ccagcaacat ttttccgccg acgttcttca cctgaatcag
    11101 cggtgaaacc agataaatca ccgcaatcgc gaagaaaaac cataagtggt aaaacactgg
    11161 cttttgcagc aggtttttca gcgctaactc cacattaatg gaggtaaaca gtgcaatgta
    11221 gagcagcgcg attgcgctat aaaaaaacag acataagccg atacgcaaga aatggcgcgg
    11281 ctgggcgctg cgttcgccaa aaaagagata gccggaaatc atgaaaaata gcggcacgct
    11341 gacacgagag gcagagttca gaacattggc gatatcccag gtgacggggc taacactatg
    11401 agcattggtc acataccagg tagtggtgtg aatcatcacc accattaaac acgctatccc
    11461 tcgcaggtta tcaatccagt aaattttggg ctgcatctgt gtctctgtat ctggttaaaa
    11521 aaagtctgac cgataaatca ttcagttggc gcactggaat aatctgagtt ttaacactac
    11581 agcttataga ggcttaagga aattcgtaag atatcagcca ctataccgat ataaataata
    11641 agactcacct gcaaaccaga cggtaattta atgatgatga actctttctt tccggcaatg
    11701 gcgcttatgg tgctagtggg ttgttctaca ccgtcacccg tgcagaaagc acaacgggta
    11761 aaggttgatc ctctgcgttc gttgaatatg gaagcgttat gcaaggatca ggcggcaaaa
    11821 cgttataaca ccggcgagca aaaaatcgac gtcaccgcct tcgaacagtt ccagggaagc
    11881 tatgaaatgc gcggttatac cttccgtaaa gagcagtttg tctgttcttt tgacgcagat
    11941 ggccattttt tgcatctttc catgcgttaa gccctgcttt ttcccgtttc atactgtata
    12001 tcttccatcc agcgggtata ctgatccctt cctttaaatc cacacgtatc cagcacgaaa
    12061 taatatgcaa aagtttgata ccaggacctt ccagggcttg atcctgacct tacaggatta
    12121 ctgggctcgc cagggctgca ccattgttca accattggac atggaagtcg gcgcgggaac
    12181 ctctcaccca atgacctgtc tgcgcgcgct ggggccagaa ccgatggcgg ctgcttatgt
    12241 tcagccttct cgtcgcccga ccgatggtcg ctacggcgaa aaccccaacc gtttacagca
    12301 ctactatcag ttccaggtgg tcattaagcc atcgccggac aatattcagg agctgtacct
    12361 tggttctctg aaagagctgg gcatggaccc gactatccac gatatccgtt tcgtggaaga
    12421 taactgggaa aacccgacgc tgggtgcctg gggactgggc tgggaagtgt ggctaaacgg
    12481 catggaagtg acgcagttca cttacttcca gcaggttggt ggtctggagt gtaaaccggt
    12541 taccggcgag atcacctacg gtctggaacg tctggccatg tacattcagg gcgtagacag
    12601 cgtttacgac ctggtctgga gcgacggccc gctgggtaaa accacctacg gcgacgtgtt
    12661 ccatcagaac gaagtggagc agtccactta caacttcgaa tacgcggatg tggacttcct
    12721 gttcacctgc tttgagcagt acgagaaaga agcgcagcag ctgctggcgc tggaaaatcc
    12781 gctgccgctg ccagcttacg agcgtattct gaaagccgcc cacagcttca acctgctgga
    12841 tgcgcgtaaa gccatctccg tcaccgagcg tcagcgctac attctgcgca ttcgcaccct
    12901 gaccaaagca gtggcagaag catattacgc ttcccgtgaa gccctaggct tcccgatgtg
    12961 caacaaagat aagtaagagg cggctatgtc tgagaaaact tttctggtgg aaatcggcac
    13021 tgaagagctg ccaccaaaag cactgcgcag cctggcagag tcctttgctg cgaactttac
    13081 tgcggagctg gataacgcag gcctcgcaca cggcaccgtt caatggtttg ctgctccgcg
    13141 tcgtctggcg ctgaaagtag ctaacctggc ggaagcgcaa ccggatcgtg aaatcgaaaa
    13201 acgcggcccg gcgattgccc aggcgttcga cgctgaaggc aaaccgagca aagcggcaga
    13261 aggttgggcg cgtggttgcg gtattaccgt tgaccaggct gagcgtctga ctaccgataa
    13321 aggcgaatgg ctgctgtatc gcgcccatgt gaagggcgaa agcactgaag cactgctgcc
    13381 gaatatggtt gcgacttcgc tggcgaaact gccgattccg aaactgatgc gctggggtgc
    13441 aagcgacgtg cacttcgtgc gtccggtgca caccgtgacc ctgctgctgg gcgacaaagt
    13501 cattcctgca accattctgg gcattcagtc cgatcgcgtg attcgcggcc accgctttat
    13561 gggcgagccg gaattcacca tcgataacgc cgatcagtat ccggaaattc tgcgtgagcg
    13621 tgggaaagtc atcgccgatt acgaagaacg taaggcgaag attaaagccg atgccgaaga
    13681 agcagcgcgt aagattggcg gtaacgctga cttaagcgaa agcctgctgg aagaagtggc
    13741 ttcgctggtg gagtggccgg tcgttctaac cgcgaaattt gaagagaaat tcctcgcggt
    13801 gccgtctgaa gcactggttt acaccatgaa aggtgaccag aagtacttcc cggtgtatgc
    13861 gaacgacggc aaactgctgc cgaactttat cttcgttgcc aacatcgaat cgaaagatcc
    13921 gcagcaaatt atctctggta acgagaaagt cgttcgtcca cgtctggctg atgccgagtt
    13981 cttcttcaac accgaccgta aaaaacgtct ggaagataac ctgccgcgcc tgcaaaccgt
    14041 gctgttccag caacagctgg gtacgctgcg tgacaagact gaccgcatcc aggcgctggc
    14101 tggctggatc gccgaacaga ttggcgctga cgttaaccac gcaacccgtg cgggcctgct
    14161 gtctaagtgt gacctgatga ccaacatggt cttcgagttc accgacaccc aaggcgttat
    14221 ggggatgcac tacgcgcgtc acgatggcga agcggaagac gtagccgtcg cgctgaatga
    14281 gcagtatcag ccgcgctttg ccggtgatga cctgccgtcc aacccggtag cttgtgcgct
    14341 ggcgattgct gataagatgg atactctggc gggtatcttc ggtatcggtc agcatccgaa
    14401 aggcgacaaa gacccgtttg cgctgcgtcg tgccgcactt ggcgtgctgc gaattatcgt
    14461 tgagaagaac ctcaaccttg atctgcaaac gctgaccgaa gaagcggtgc gtctgtatgg
    14521 cgataagctg actaatgcca acgtagttga tgatgttatc gactttatgc tcggtcgctt
    14581 ccgcgcctgg tatcaggacg aaggttatac cgttgacacc atccaggcgg tactggcgcg
    14641 tcgtccgact cgtccggctg atttcgatgc ccgcatgaaa gcggtatcgc acttccgtac
    14701 cctggaagca gctgcagcac tggcggcggc gaacaaacgt gtatctaaca tcctggcgaa
    14761 atctgacgaa gtgctgagcg atcgcgtgaa tgcctctact ctgaaagagc cggaagaaat
    14821 taaactggcg atgcaggttg tggtgctacg tgacaagctg gagccgtact ttgcggaagg
    14881 tcgttaccag gatgcgctgg tcgaactggc agagctgcgt gaaccagtcg atgccttctt
    14941 cgataaagtg atggtcatgg ttgatgacaa agaattgcgt atcaaccgtc tgaccatgct
    15001 ggagaaactg cgcgaactgt tcctgcgcgt tgcggatatt tcgctgttgc agtaataacg
    15061 ccgttattaa aaagcctgcc atctggcagg ctttttttat cgctaaataa tacagcaacc
    15121 tttaataatc ttctgctgaa taaagattat ctcatatatt aattttatga gattttttta
    15181 ggattatatc aaggagaaga aacaaactta ttaagctaga atagccacgg gtgcttgaga
    15241 ctgtttgtct caggtattca ccgaaaggca gacagagaaa agccccacct gactataaat
    15301 caaagtgagg cccctctatg tatagacacc ataaggttag cctcttactc gttggaaatc
    15361 aacacagggg gctgggaatg ccgcagaaat atagattgct ttctttaata gtgatttgtt
    15421 tcacgctttt atttttcacc tggatgataa gagattcact gtgtgaattg catattaaac
    15481 aggggagtta tgagctggcg gcatttttag cctgcaattt gaaagagtaa gagtcttcgg
    15541 cggaaagtta ttcccgcctt tcttacggcg ttgcgcattc tcattgcacc caaatttatt
    15601 tttcacaaaa ataatactag attttattac gcgatcgatt atttatttcc tgaaaaacaa
    15661 ataaaaaaat ccccgccaaa tggcagggat cttagattct gtgcttttaa gcagagatta
    15721 caggctggtt acgttgccag ctgccgggcc tttagcgccg ctttcgatgg tgaaggacac
    15781 tttctgacct tcgtccagag atttgtaacc atcgttctgg atagcagaga agtgtacgaa
    15841 cacgtcttta gagccatcgt caggagtgat gaagccgaag cctttgtcag cgttgaacca
    15901 ttttacgata ccagtcattt taccggacat agtgtattac ctttaataat taagtgtgcc
    15961 tttcggcgat atggcgtgct ttacagattt tgaagcgtta aaggaatgtg cactacgagg
    16021 ggtatcaacg ataactcttg aagggacttg ccttactaca ctgctttaat ggtctgtacg
    16081 tcaaaccgtt gatgtgcatt aagccacgca ttggcgggtg atgcaacaat tatttttcat
    16141 atttatgatt aatcggttgg aaaacggtcc tgtcatcagg accgtaaaca gcaataaagt
    16201 ggataaaagt ctattccatc aactgcttac ttaatgccgg gttggcttga atcaaacgca
    16261 tcaattttag ttcggcactt gaaggcttca cgcgtctgga ttcccattcc tttaccatgg
    16321 cgactgatac gcccaaaacc cgggcgaaat catcgatttt taatcctgtc ccttttcgta
    16381 actgctcaat ttcggtacag gacgttgttc tgtgcgtcag ggtaattttc tgcgtttcat
    16441 ctttaaaaac aatctgttcc aggctgctca acagctcatg cattggatct ttatattcca
    16501 ttgaaaactc ctcaaatcac actgcgggat cgtgaattgc atcgaagctc attaagaata
    16561 gtcgggaaaa gcagacacag cggggtgaag tcagggcatg ctctggctat gattcgattc
    16621 agccagtcat ttagcgtgac ttatagccac ctaacgatct gataaatgcc gatatatctc
    16681 taaccactcc tggtttgtaa agatttcgaa aatctaacct tctgcgaagg cgtaaatctc
    16741 tggctacaaa aaaggcaacg gcaatatcat tttttaacaa tgaaacaaca aattaacctt
    16801 gtttcatttt ctccatgatt ttaccaggaa gattacgtag aaagggtatc ctgcaagcct
    16861 gtcctggact atccttgtca cgtcagacac gcgtgtgtcg ttgtgcgctt tttttgggtg
    16921 aaaggagtaa gaaaatggcg acaggaaagt cctgctctcg ctggtttgcg cctcttgcgg
    16981 cgttattaat ggtagttagc ctgagtgggt gttttgataa agaaggcgat cagcgtaaag
    17041 cgtttatcga cttcctgcag aatacagtga tgcgtagcgg tgaacgtcta ccaaccctga
    17101 ctgccgatca gaaaaaacag tttggtcctt ttgtctctga ttacgcgatt ctgtatggtt
    17161 attctcagca ggtgaatcag gcgatggatt ccggtctgcg cccggttgta gacagcgtta
    17221 acgcaattcg cgtaccgcag gattacgtta cgcaaagcgg tccgctgcgt gaaatgaatg
    17281 gttcgctggg tgtgctggcg caacaactgc aaaatgcgaa gttgcaggcc gatgctgcgc
    17341 actctgcgct aaaacagagt gatgacctga aaccggtctt tgatcaggca ttcaccaaag
    17401 tggtaacgac gccagctgat gcattgcaac cgttgattcc ggcggcacaa actttcacgc
    17461 aacaactggt gatggttgga gactacattg cccaacaagg tactcaggtg agctttgtag
    17521 caaatggcat ccagttcccg acttcacagc aggcaagtga atataacaaa ctgattgcgc
    17581 cattaccggc acaacatcag gcgtttaatc aggcctggac tacagcagtt actgcaaccc
    17641 aataaagagt aaaagcccga gcgattatcg ggctttactc cacgcagtcg cggcttagtc
    17701 cgcgacgtgc ggattcacac agttcttctc aacctttcct tgtaacgcat caatcaaatt
    17761 atccacggca caggcggcca tgccataacg cgtctcatgg gtggcagatc caatatgcgg
    17821 tactgcgacg acgttggcca ttgagagcaa cggcgaatct acggaaagtg gctcttgttc
    17881 gaagacatcc agcccggcgg cgtgaatttc ccctttctgc aatgctgcga tcagtgcatt
    17941 ttcgtcaacc accgggccac gtccggcatt aatgaaaatg gcggaggatt tcattttggc
    18001 gaattgttct gcgccaaaca gatgatgcgt ctcatcagtt aacggcagga tcaggcaaac
    18061 gaaatctgac tcttgtaaca gtgtatccaa atcgcagtag cgggcgttga agcgttcttc
    18121 tgcttcttta tggtggcggc gcgcgttata gaggatgggc atgttgaagc caaagtgcgc
    18181 acgttgtgcc agcgccatac cgatccgtcc catcccgaca atgcccagtg ttttatggtg
    18241 aacgtcagtg ccgtaccagt ccgggcctat gctcgcggtc cattcgcctg cttttacccg
    18301 ttctgctacc tccacaaccc gacgagcggt agacaacacc agcgccatca gcgtatcggc
    18361 gacggtttct gttaagacgg ttggcgtgtg catcagcaga atttttcggg cggtaagcgc
    18421 atcgacatca aaattgtcat agccgacgga gatcgttgat gtggcacgca gtttcggcat
    18481 tttttccagc aatgcggcat caacattctc gtttgaaccc agtaaacctt cagcttcggc
    18541 aaaaattgct gcattttgtt cgacggtttg tgggctgagg tttgccacct ggtgaacggt
    18601 gaaatgctct tgcaggcgtt gcagtaaatc atcaggtaag gctttgtaga ggataacgga
    18661 cggcttcatg cttctctcca ttcactgata aggcgatcac cttagcctga aatatgatga
    18721 aggaaaactg aagaatgcca ggtgatttct gcatcacctg gcatgaaagg gattacagcg
    18781 ggcttaaggt aatttctaca cggcggtttt gcgccttacc ttctgcggtg ctgttgctgg
    18841 cgattgggtt agccgggcca aggccctgag tacggatgcg gctggcgtcc acgccctggg
    18901 tgatcaacgc gctggcaacg gaatccgcac gctgctggga gagacgcatg ttcagatcgt
    18961 gaccacccgt gctgtcagta taaccaatca cgttaaccgc cgttttcgga tactctttca
    19021 gcaccattgc cacgccggtc agggtgttag cgcccgccgg tttcagggtt gcgctgctgc
    19081 tgtcgaaggt cacattgttc ggcatattga ggataatgtt atccccgctg cgggttacgc
    19141 taacaccagt gccgcgcatt ttgtcacgca gcttcgcttc ctgcacatcc atgtaataac
    19201 ccacgccgcc gcccagagct gcgcctgctg ctgcgccaat cagcgcgcct ttaccgcgat
    19261 ctttcttcga agaagagagc gcaccaatac ccgcgcccac gagagagccc agacctgcgc
    19321 cgatagcaga tttacctgct tcgcgttcgc cggtgtaagg gttagttgtg cagccagata
    19381 ccgccagagc gccactcact acggcggcaa taagataaac acgtttcttc attgttaatc
    19441 cttaataacc tttttattct ttgccacggg ttccgtggcg ggagattatg ccgcgtgaac
    19501 atgaagatta ttcctgggaa tactcggaaa tttgtaagta atatttaact gctcaataca
    19561 tctaaccttt caggagtctt cggtttgacc aactcatcct cacgatattc cgttctgact
    19621 gccgcccatt gggggcccat gctggttgaa accgacggcg aaaccgtgtt tagctcgcgt
    19681 ggcgcgttag ccacaggaat ggaaaactcc ttgcagagcg cggttcgtga ccaggttcac
    19741 agcaatacgc gggtacgatt tccaatggtg cgcaaaggct ttctcgcgtc accggaaaac
    19801 ccgcaaggca ttcgtgggca ggatgaattt gttcgcgtga gttgggatga ggcgctggat
    19861 cttattcacc aacaacataa acgcattcgt gaggcttatg gtccggcatc gatttttgct
    19921 ggttcctacg gctggcgttc aaacggcgtg ctgcataagg cctcgacatt attacaacgc
    19981 tatatggcgc tggcaggcgg ttataccggg catctggggg attattcgac cggcgcggca
    20041 caggcgatca tgccgtatgt cgtgggtggt agtgaagttt atcaacagca gaccagttgg
    20101 ccgctggtgc tggaacatag cgatgtcgtg gtgctgtgga gtgctaaccc actcaatacg
    20161 ctgaaaattg cgtggaatgc atccgatgag caggggcttt cttacttttc tgcactgcgt
    20221 gacagcggga aaaagctgat ctgcattgat ccaatgcgat cggaaaccgt cgatttcttt
    20281 ggcgataaaa tggagtgggt ggcaccgcac atgggcactg atgttgcgct gatgctgggg
    20341 atcgcccata cgctggtgga aaatggttgg cacgacgaag cgtttctggc gcgttgcacc
    20401 accggttatg ccgtcttcgc ctcttatttg ctgggcgaga gtgacggaat agcgaaaacc
    20461 gccgaatggg cagcagagat ttgtggtgtt aacgcagaga aaatccgcga gctggcggct
    20521 attttccacc aaaataccac catgctgatg gctggctggg gaatgcagcg ccaacagttt
    20581 ggtgagcaaa aacactggat gatcgtcacg ctggcagcaa tgttggggca aatcggcaca
    20641 cccggcggcg gttttggtct ttcttaccat tttgccaatg gtggtaaccc cacgcggcgc
    20701 gctgcggtac tctcttccat gcagggcagc ttgccgggtg gctgcgatgc ggtagataaa
    20761 atccctgtcg cccgcattgt tgaagcactg gaaaaccccg gtggcgcata tcaacacaac
    20821 ggtatggacc gacatttccc ggatattcgt tttatctggt gggcgggcgg tgccaacttt
    20881 actcatcatc aggataccaa tcgcctgatc cgtgcctggc aaaaaccgga gctggtggtg
    20941 atctctgaat gcttctggac ggcggcggca aaacacgcgg atatcgttct gcctgcgact
    21001 acctcttttg agcgtaatga tctcaccatg accggtgatt acagtaatca gcatctggtg
    21061 ccgatgaagc aagtggtgcc gccacgctat gaagcgcgta atgattttga tgtttttgcc
    21121 gagttaagtg aacgctggga gaagggcggt tatgcacgtt ttacggaagg aaaaagtgag
    21181 ctgcaatggc tggaaacgtt ttataacgtt gcccggcagc gcggggcaag ccagcaggtt
    21241 gaattgccgc catttactga gttctggcaa gccaaccagt taattgagat gccggaaaac
    21301 ccggacagcg agcggtttat tcgcttcgct gatttttgcc gcgatccgca ggcgcatcca
    21361 ttaaaaaccg ccagcggtaa gattgaaatc ttctcgcagc gtattgccga ttacgcttat
    21421 ccggattgcc cggggcatcc aatgtggctg gtgccggacg aatggcaggg caatgccgaa
    21481 ccggaacagt tacaggtact ttctgctcat ccggcgcatc gtctgcacag ccagctgaat
    21541 tacagttctc tgcgcgaatt gtacgcggtg gcaaaccgtg agcctgtcac cattcatcct
    21601 gacgatgccc aggcgcgcgg catacaagat ggcgatactg ttcggctgtg gaacgcacgc
    21661 gggcaaattc ttgccggagc ggtcattagc gagggaatta aacctggcgt gatttgcatt
    21721 cacgaagggg catggccgga tctggattta accgctgacg gtatttgtaa aaacggcgca
    21781 gtgaacgtgc tgaccaaaga tctccccagc tcgcggctgg ggaatggctg tgcgggtaat
    21841 acggcgctgg catggctaga aaaatacaac ggtccggaac tgacacttac agcgtttgaa
    21901 ccaccggcca gctcataatc caggtgggta gttgggtttc atcctgccat gcgcaatcga
    21961 caatgtgaaa accctgcgcc tggtaaaaat ctatcgccgg ttgatttttt tgataaacct
    22021 ccagcatcag atggggatag cgctgctgca catactgcat cagcgcctta ccaataccgc
    22081 gtctgacggc cttcggtgcg acaaacattg ctgccagaaa tcggccttcc ataatgctga
    22141 caaaaccgag aagcttaccg tcttcttccc agacccagtt tttcgcattg gcaagatacg
    22201 catcccgcac cagcggaatg cactcgcgcc agtaattcac ttttataaag ggatgccccc
    22261 aggttgtact ttccagccac agttcgagga tcgcggggag ttctgaacgt tgcgcttccc
    22321 gaatcatggt ttatttcccg gatagcaaca gcagccaacc acatgatcat tcaccagccc
    22381 acatgcctgc ataaaggagt aacagattgt ggtaccgaca aacttaaaac cacgtttttt
    22441 cagtgcctta gatagggcgt cggaggcggg cgtagatgtg gggatttcgc tcaacgttgt
    22501 ggcttgtgtc acctgtggct gatgatttac aaacgaccag acaaagtcgg caaacggttc
    22561 gccgttctgt tccatttgca ggtacgcccg cgcattacca ataattgcct gaattttccc
    22621 gcgatggcgg ataatcccgg cgtcctgtac cagtctttcg acatcctctt cctgcattgc
    22681 ggcaaccttc accggatcga actgatgaaa gcaggcgcga tagttttcgc gttttttgag
    22741 gacggtgatc cacgataacc cagcctgctg cccttccagg cagatcattt cgaacagttt
    22801 tttcctgtca gtttcaggca cgccccactc attatcatgg taggcaatat aaagcgggtc
    22861 ctgactcacc cagccgcaac gttccatact ttccctcgct atgtacccga tcgtaaaaat
    22921 ttcagccgct tttcttgacg catcggtgaa aaagtcaata tttaatacag ggctactatg
    22981 cccgatatcc ttcatacaat gacaataata ttgccgaatt cggctcttcc ctggagtcgt
    23041 gttaatgatc ataaaaaaaa gcggtggtcg ctggcagcta agcctgctgg cgagtgtggt
    23101 aatcagtgcc ttttttctca acacagctta cgcctggcaa caagaatata tcgttgatac
    23161 gcaacccggg cattccacag agcgttacac ctgggatagt gatcatcaac ctgattacaa
    23221 cgatattttg tcgcaacgta ttcaaagtag ccaaagggcg ctgggactgg aagtcaatct
    23281 ggcggaagaa acccctgtgg atgtgaccag cagtatgagt atgggctgga attttccttt
    23341 atatgaacag gttacaactg gcccggtcgc ggcattacat tacgatggca caaccacctc
    23401 gatgtataac gagtttggcg acagtactac cacgctgacc gatccgttat ggcatgccag
    23461 cgtgagtacc ttaggctggc gtgttgactc ccggcttggc gatctccgac cctgggcgca
    23521 aatcagctat aaccagcaat ttggcgagaa tatctggaag gcgcaatcag gcctgagccg
    23581 gatgacggcg acaaaccaga acggcaactg gctggatgtc accgtaggcg ctgatatgtt
    23641 gctcaatcaa aatattgccg cctatgccgc gctatctcag gcagaaaata ccactaacaa
    23701 tagcgattat ctgtatacga tgggggttag tgccagattt taacgtaaca atcacaattg
    23761 aaaccattga ataacaatag ttgtggcgat agtgggtgct aacttaccaa ataataaatt
    23821 tggtgaataa ttgtcgcgtc attcattcct gaactaaggc atttcattcc gttctgatgg
    23881 catttcatgc cgtttttccc caggcataaa gtgcacttcg ttatggttgt cggcagagat
    23941 ttttcctttt tattactgca ggaatactgc catgacacct tcaaattatc agcgtacccg
    24001 ctggttgaca ctcatcggta ctatcattac ccagtttgcg ctggggtcgg tttatacctg
    24061 gagcctgttt aacggcgcgc tttccgccaa gctggatgcg ccggtaagcc aggtcgcttt
    24121 ctctttcggc ttgttaagtc tggggctggc aatatcgtct tctgttgcgg gcaaattgca
    24181 ggaacgtttt ggtgttaaac gcgtcaccat cgcttccggc attttgctgg gattagggtt
    24241 cttcctgaca gcgcattctg acaacctgat gatgctgtgg ttaagcgccg gtgtgctggt
    24301 cggtctggcg gatggcgcgg gttacctgct gacgctctct aactgcgtga aatggttccc
    24361 ggagcgtaaa ggtctgatct ccgcgttcgc tatcggttct tatggtctgg gtagcctggg
    24421 tttcaaattt atcgacacgc agctgctgga aacggtcggt ctggaaaaaa cctttgtgat
    24481 ttggggagcg attgcgctgg tgatgattgt tttcggcgca acgttaatga aagacgcacc
    24541 aaaacaggaa gtgaaaacca gcaatggtgt ggtggagaaa gattacacgc tggcagagtc
    24601 gatgcgtaaa ccgcagtact ggatgttagc ggtaatgttc ctgactgcct gcatgagcgg
    24661 cctgtacgtg attggggtag cgaaagatat cgcccaaagt ctggcacacc ttgatgtggt
    24721 ttctgcagcc aatgcagtca ctgttatttc catcgccaac ctttcaggtc gtctggtgct
    24781 gggcattctg tctgacaaaa tcgcccgtat ccgtgttatc accattggtc aggtaatttc
    24841 gctggtgggt atggcggccc tgctgtttgc accattgaat gcagtgacgt tctttgcagc
    24901 gattgcctgc gtggcattta actttggcgg cactattacc gtctttccgt cactggtcag
    24961 tgagttcttc ggcctcaata acctggcgaa aaactacggt gtgatttacc tcggtttcgg
    25021 tatcggtagc atttgtggtt cgattatcgc ctcactgttt ggcggcttct atgtgaagtt
    25081 ctacgtcatc tttgctctgc tgattctgtc attggcgctt tctacgacga ttcgtcagcc
    25141 agagcagaaa gtattgcgtg aggcgcatat ttagccagaa actacaggtt ttgaacgaaa
    25201 gggggaggaa atcctccttt tttattgcat tttaaagaat aaaaatgaaa atattttatc
    25261 ttattatttg cgtggtgaat gtttttgttt tgtttaattg atattttata tttatttatt
    25321 cctaa
//
LOCUS       DAZKIP010000061        24863 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZKIP010000061 DAZKIP010000000
VERSION     DAZKIP010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.061"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            269..772
                     /gene="ftnB"
                     /locus_tag="ACHOUK_002720"
     CDS             269..772
                     /gene="ftnB"
                     /locus_tag="ACHOUK_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFP9898137.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(852..1103)
                     /gene="yecJ"
                     /locus_tag="ACHOUK_002721"
     CDS             complement(852..1103)
                     /gene="yecJ"
                     /locus_tag="ACHOUK_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFP9898138.1"
                     /translation="MSQTLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1218..1304)
                     /gene="azuC"
                     /locus_tag="ACHOUK_002722"
     CDS             complement(1218..1304)
                     /gene="azuC"
                     /locus_tag="ACHOUK_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFP9898139.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1566..1889
                     /gene="yecR"
                     /locus_tag="ACHOUK_002723"
     CDS             1566..1889
                     /gene="yecR"
                     /locus_tag="ACHOUK_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFP9898140.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGISDRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            2060..2557
                     /gene="ftnA"
                     /locus_tag="ACHOUK_002724"
     CDS             2060..2557
                     /gene="ftnA"
                     /locus_tag="ACHOUK_002724"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFP9898141.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(2594..2833)
                     /gene="yecH"
                     /locus_tag="ACHOUK_002725"
     CDS             complement(2594..2833)
                     /gene="yecH"
                     /locus_tag="ACHOUK_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFP9898142.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            3025..4236
                     /gene="tyrP"
                     /locus_tag="ACHOUK_002726"
     CDS             3025..4236
                     /gene="tyrP"
                     /locus_tag="ACHOUK_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFP9898143.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(4298..4963)
                     /gene="yecA"
                     /locus_tag="ACHOUK_002727"
     CDS             complement(4298..4963)
                     /gene="yecA"
                     /locus_tag="ACHOUK_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFP9898144.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(5159..5245)
                     /locus_tag="ACHOUK_002728"
     tRNA            complement(5159..5245)
                     /locus_tag="ACHOUK_002728"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5209..5211),aa:Leu,seq:taa)
     gene            complement(5258..5331)
                     /locus_tag="ACHOUK_002729"
     tRNA            complement(5258..5331)
                     /locus_tag="ACHOUK_002729"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5297..5299),aa:Cys,seq:gca)
     gene            complement(5385..5460)
                     /locus_tag="ACHOUK_002730"
     tRNA            complement(5385..5460)
                     /locus_tag="ACHOUK_002730"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5425..5427),aa:Gly,seq:gcc)
     gene            complement(5612..6160)
                     /gene="pgsA"
                     /locus_tag="ACHOUK_002731"
     CDS             complement(5612..6160)
                     /gene="pgsA"
                     /locus_tag="ACHOUK_002731"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFP9898145.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(6217..8049)
                     /gene="uvrC"
                     /locus_tag="ACHOUK_002732"
     CDS             complement(6217..8049)
                     /gene="uvrC"
                     /locus_tag="ACHOUK_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFP9898146.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(8046..8702)
                     /gene="uvrY"
                     /locus_tag="ACHOUK_002733"
     CDS             complement(8046..8702)
                     /gene="uvrY"
                     /locus_tag="ACHOUK_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFP9898147.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            8844..8960
                     /locus_tag="ACHOUK_002734"
     CDS             8844..8960
                     /locus_tag="ACHOUK_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9898148.1"
                     /translation="MVTPVTNGLPDRFTVSPATKCDKNYLYSAKMKISPLNM"
     gene            8998..9174
                     /gene="yecU"
                     /locus_tag="ACHOUK_002735"
     CDS             8998..9174
                     /gene="yecU"
                     /locus_tag="ACHOUK_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFP9898149.1"
                     /translation="MIKIFIGHYINAFNSTVDGTLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            9161..9385
                     /gene="yecF"
                     /locus_tag="ACHOUK_002736"
     CDS             9161..9385
                     /gene="yecF"
                     /locus_tag="ACHOUK_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFP9898150.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEQQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(9453..10175)
                     /gene="sdiA"
                     /locus_tag="ACHOUK_002737"
     CDS             complement(9453..10175)
                     /gene="sdiA"
                     /locus_tag="ACHOUK_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFP9898151.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMETAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFNE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILRWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(10405..11157)
                     /gene="tcyN"
                     /locus_tag="ACHOUK_002738"
     CDS             complement(10405..11157)
                     /gene="tcyN"
                     /locus_tag="ACHOUK_002738"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFP9898152.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARKLLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPEQPRTRQFLEKFLLQ"
     gene            complement(11154..11822)
                     /gene="tcyL"
                     /locus_tag="ACHOUK_002739"
     CDS             complement(11154..11822)
                     /gene="tcyL"
                     /locus_tag="ACHOUK_002739"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFP9898153.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     TAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(11837..12823)
                     /gene="dcyD"
                     /locus_tag="ACHOUK_002740"
     CDS             complement(11837..12823)
                     /gene="dcyD"
                     /locus_tag="ACHOUK_002740"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFP9898154.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASVEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(12928..13728)
                     /gene="tcyJ"
                     /locus_tag="ACHOUK_002741"
     CDS             complement(12928..13728)
                     /gene="tcyJ"
                     /locus_tag="ACHOUK_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9898155.1"
                     /translation="MKLAHLGRQALMGVMAVALIAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(13816..14367)
                     /gene="fliZ"
                     /locus_tag="ACHOUK_002742"
     CDS             complement(13816..14367)
                     /gene="fliZ"
                     /locus_tag="ACHOUK_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFP9898156.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRISLVRDGKIV
                     NKIEISRLDTLLDENGWQTEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(14413..15132)
                     /gene="fliA"
                     /locus_tag="ACHOUK_002743"
     CDS             complement(14413..15132)
                     /gene="fliA"
                     /locus_tag="ACHOUK_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFP9898157.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(15452..17209)
                     /locus_tag="ACHOUK_002744"
     CDS             complement(15452..17209)
                     /locus_tag="ACHOUK_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HFP9898158.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQATTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGETITIDL
                     KKIDSDTLGLNGFNVNGKGTITNKAATVSDLTSAGAKLNTTTGLYDLKTENTLLTTDA
                     AFDKLGNGDKVTVGGVDYTYNAKSGDFTTTKSTAGTGVDAAAQAADSASKRDALAATL
                     HADVGKSVNGSYTTKDGTVSFETDSAGNITIGGSQAYVDDAGNLTTNNAGSAAKADMK
                     ALLKAASEGSDGASLTFNGTEYTIAKATPATTTPVAPLIPGGITYQATVSKDVVLSET
                     KAAAATSSITFNSGVLSKTIGFTAGESSDAAKSYVDDKGGITNVADYTVSYSVNKDNG
                     SVTVAGYASATDTNKDYAPAIGTAVNVNSAGKITTETTSAGSATTNPLAALDDAISSI
                     DKFRSSLGAIQNRLDSAVTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGN
                     SVLAKANQVPQQVLSLLQG"
     gene            17475..18872
                     /gene="fliD"
                     /locus_tag="ACHOUK_002745"
     CDS             17475..18872
                     /gene="fliD"
                     /locus_tag="ACHOUK_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFP9898159.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSSTTAFSATTAGNAIAGKYTISVTHLAQA
                     QTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNAKAG
                     VSASIINVGNGDYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEVSVA
                     AQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIKDWV
                     NAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVSSSS
                     YKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGSNLT
                     SWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSLNST
                     SSYLTQQFENNSNSK"
     gene            18897..19307
                     /gene="fliS"
                     /locus_tag="ACHOUK_002746"
     CDS             18897..19307
                     /gene="fliS"
                     /locus_tag="ACHOUK_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFP9898160.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVETLMRNIADAWKESLLSPSLIQDPV"
     gene            19307..19672
                     /gene="fliT"
                     /locus_tag="ACHOUK_002747"
     CDS             19307..19672
                     /gene="fliT"
                     /locus_tag="ACHOUK_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFP9898161.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVDPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            19750..21237
                     /gene="amyA"
                     /locus_tag="ACHOUK_002748"
     CDS             19750..21237
                     /gene="amyA"
                     /locus_tag="ACHOUK_002748"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFP9898162.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKVQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPNEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRNYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVL"
     gene            complement(21271..21684)
                     /gene="yedD"
                     /locus_tag="ACHOUK_002749"
     CDS             complement(21271..21684)
                     /gene="yedD"
                     /locus_tag="ACHOUK_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFP9898163.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            21871..23076
                     /gene="yedE"
                     /locus_tag="ACHOUK_002750"
     CDS             21871..23076
                     /gene="yedE"
                     /locus_tag="ACHOUK_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFP9898164.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVAAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            23073..23306
                     /gene="yedF"
                     /locus_tag="ACHOUK_002751"
     CDS             23073..23306
                     /gene="yedF"
                     /locus_tag="ACHOUK_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFP9898165.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            23415..24083
                     /gene="yedK"
                     /locus_tag="ACHOUK_002752"
     CDS             23415..24083
                     /gene="yedK"
                     /locus_tag="ACHOUK_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFP9898166.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAREWMRQEISGKEASEIAASGCVPANQFSWHPVSRAVGNVKNQGAEL
                     IQPV"
     gene            24194..24673
                     /gene="yedL"
                     /locus_tag="ACHOUK_002753"
     CDS             24194..24673
                     /gene="yedL"
                     /locus_tag="ACHOUK_002753"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9898167.1"
                     /translation="MFTIKTDDLTHPAVQALVAYHISGMLQQSPPESSHALDVQKLRN
                     PTVTFWSVWEGEQLAGIGALKLLDDKHGELKSMRTAPNYLRRGVASLILRHILQVAHD
                     RCLHRLSLETGTQAGFTACHQLYLKHGFVDCEPFADYQLDPHSRFLSLTLCEDNELL"
BASE COUNT         6177 a         6299 c         5959 g         6428 t
ORIGIN      
        1 gattaatatc aacaagatac aaaaagcact atcattaaaa ttcattgcag ttacattgat
       61 ttcatcaatg aaatgtaaaa atatataaac ttgatgattt aagcattttc ttatacccgt
      121 tcagacgtta ttcttatttc agatcatcgt cagaattgac tccacgatca catttcggac
      181 cggcagaaag gaattattct gcaaacagta attatggtgt tttgatttat cttgcacctc
      241 tccacttctg gatataagga tattaggtat ggcaaccgct ggaatgcttc tcaaactcaa
      301 ctctcaaatg aaccgcgagt tttacgcatc caatctctac cttcacctga gtaactggtg
      361 ttctgaacag agtctgaacg gcaccgccac tttccttcgc gcccaggcac agagtaatgt
      421 gacccaaatg atgcgcatgt ttaactttat gaagagtgtc ggcgctaccc ccatcgttaa
      481 agccattgat gttcccggtg aaaaactgaa ctctctggaa gaactgttcc aaaaaacgat
      541 ggaagaatac gagcaacgtt ctagtacgtt ggcacagtta gccgatgaag cgaaagaact
      601 gaatgatgat tcaaccgtca atttcctgcg cgatctggaa aaagaacagc agcatgatgg
      661 cctgttgctg caaaccattc ttgatgaagt gcgcagtgcg aaacttgcgg gtatgtgccc
      721 agtgcagacc gaccaacatg ttctgaatgt cgtgtcgcat cagctgcatt gatcatacat
      781 cgacgctaat gcattgcacc gatgaagttt ttgagaaacc gctgcctcat cttttttgaa
      841 gcagcggttt tttaatggga ttcgccttgt ggggtaaact tgagctcaat cagcgcgatg
      901 gctttttgga ttgcccgcat ggtgaccggg tctgcggcag cgggatgatg agtaaagtcg
      961 atattcttca gctgactgga cattttttca cgaacttcaa cgggcgcgat tacatcgaga
     1021 acatccagaa tttgtttgat aaccaactgg caagcaacca catcagaaac cagttcctga
     1081 tcggcattca gcgtctggga catcgtaaac tcctgatagc attttgaaag ccgttatagt
     1141 agcggcttca catcttcaac gatagtcaca tcccccctca tcaggacaca aaaaaacctg
     1201 ccggagcagg ttttttgtta tcggaacata ttgcctggcg gtacgtcttt gaacgtcttg
     1261 caatagttat tgaacatact tttcaggatt ttgcgcagtt tcatcgcggc actccgacca
     1321 tttgttatac aggtgttatt gtctttgcgc ttataatatg acaaccatca caaaaatcaa
     1381 tctttatgtg atacaaatca cataaatacc cctttaatgt tataaaaatg ataatcaaag
     1441 aacagccccc tatttctgac acctacagat ggcaagaaat agcgcctgcc aggcgtcttt
     1501 tccggccatt gtcgcagcgc tgtaacgcgt aaaatagtgc tttctcttac tcttctggct
     1561 ggaccatgag acttctgatt ctgactcttt cattaataac gctggcgggg tgtacggtta
     1621 ctcgccaggc ccatgtgagc gaagttgacg cggcaacagg tattgtacgg ttggtttatg
     1681 accaggcttt tttgcagcat gcccataccg atcgttatgt cagtcgcggg atctccgatc
     1741 gcgcatgcca acaggaaggc tatacccacg cgatcccctt tggtcagcca gtaggcaact
     1801 gtagcctttt tgcgggttcc ttatgtttga atacggaatt cactttgtca tatcagtgcc
     1861 atcattccgc cttccctgtc tttctctaaa tattttaatc agcgagggga tcttcgctga
     1921 ttaaagaaat agcggtaatg cgttttattc ccattcgtat ttttaataat tgaagtttat
     1981 attttacctt ttgcaaataa taaaataaca aattatagtg acgccacagc aacaaatata
     2041 acctttgtgg agcactatca tgctgaaacc agaaatgatt gaaaaactta atgagcagat
     2101 gaacctggaa ctgtactctt cactgcttta tcagcaaatg agcgcctggt gcagctatca
     2161 taccttcgaa ggtgctgccg cgttcctgcg ccgtcacgcc caggaagaga tgacgcatat
     2221 gcagcgtctg tttgattacc tgactgatac cggcaattta ccgcgtatta ataccgttga
     2281 atctccgttt gctgaatatt cctcacttga tgaattattc caggaaacct ataaacacga
     2341 acaattaatc acccagaaaa ttaacgaact ggctcatgct gcaatgacca atcaggacta
     2401 cccaacattt aatttcctgc agtggtatgt ttctgagcag catgaagaag agaaactgtt
     2461 caaatcgatt attgataaat taagcctggc aggcaaaagc ggcgaaggtc tgtattttat
     2521 cgacaaagaa ctctctaccc tcgacacaca aaactaatgc tctccggcgg caggtttctg
     2581 ccgccatttc aaatcagtga cggcaaatct tactctgatc ggtcgaaaaa ccttcttctg
     2641 aaggtataaa tttgcctttt gctgccagaa acgctaccag ctctcccgct gtcatccctt
     2701 ctgccgagca agtgtgaaaa cgtgcctgtt caccaaaacg cgctttaatc gcagcttcca
     2761 gactggcatg cgtatattgc tcgcctgatt caatcatcat atttaacact tcatgaccgt
     2821 gaatagagtc catcgttcct cctcaaaaaa aagcctagcg tagcgattgc cgcttatgaa
     2881 gactttgcgc cagcgcagga ctgaatgctt tttattgtac atttatgttt acaccatatg
     2941 taacgtcggt ttgacgaagc agccgttatg ccttaacctg cgccgcagat atcactcata
     3001 aagatcgtca ggacagaaga aagcgtgaaa aacagaaccc tgggaagtgt ttttatcgtg
     3061 gcgggaacca caattggcgc aggcatgctg gcaatgccgc tggctgcggc cggtgttggt
     3121 tttagcgtta cgttaatctt gttgattggg ctttgggcgt tgatgtgcta cacggcgcta
     3181 ttactgctgg aggtgtacca gcatgttccg gcagataccg gtctgggcac gctggcaaaa
     3241 cgctatctgg gacgctacgg tcaatggctg acgggcttca gtatgatgtt cttaatgtat
     3301 gctctgactg cggcatacat cagcggtgcc ggtgaattgt tggcctccag catcagcgac
     3361 tggacaggta tttcaatgtc ggcaaccgct ggcgtgttgt tgttcacttt tgttgccggt
     3421 ggcgtggttt gtgtcgggac atcgctggtc gatttattta accgttttct gttcagcgcc
     3481 aaaattattt ttctggtggt gatgctggta ttgctgctac cgcatattca caaagtgaat
     3541 cttttaaccc tgccgttgca acaggggctg gcactgtctg caatcccggt gatttttacg
     3601 tcgtttggtt ttcacggtag cgtgccgagt attgtcagct atatggatgg caacattcgt
     3661 aagctacgct gggtgtttat aaccggtagt gcgatccccc tggtggcata tattttctgg
     3721 caggtggcga cgcttggcag cattgattca acaaccttta tgggattgct ggctaatcat
     3781 gccggattaa acgggctgtt acaggcgtta cgcgaaatgg tggcctctcc gcatgttgag
     3841 ctggcagtgc atttatttgc tgatttagcc cttgccacgt catttctcgg cgttgcgtta
     3901 ggcttatttg attatctggc agatttgttt cagcgttcaa ataccgttgg tggacgattg
     3961 caaactggag caattacctt tctgccaccg ttggcgtttg cactgtttta tccacgagga
     4021 tttgtgatgg cgctgggtta cgccggtgtg gcgctggcgg tactggcatt gattatccct
     4081 tcgctgttga cctggcaaag cagaaagcac aatcctcaag cgggttaccg ggtcaaaggt
     4141 ggtcgtccgg cgctggtggt ggtgtttctc tgtggtattg ctgtgattgg cgtgcaattt
     4201 ttgattgcgg cagggttgtt accagaagtg gggtgattag atagcctcaa attccttatt
     4261 gggtgccaga attaacgctg acacccaatt tgacttctta atgcaggcag cactgcttaa
     4321 attttttacc actaccgcac gggcaaggat cgttacgccc cggtttctct tctgctttga
     4381 tcggttgctg aacagctttt tcctgcggat gcgccatcca gtacgcatgt agatcaagcg
     4441 ccgccagtcg aatggcatct acgctctctt caaacgcttc tggcgacatc ttctctaccc
     4501 gctcgaagtt ttcctcagta ccgtgcagcg ctatcgcctc cagcgctggt tttaacgaat
     4561 cgggcaatgt tgaccagtca gaaagtgcca cgccccgcat atagccaaag caccactcct
     4621 caacaatcgt cagctcgctg ccatcaactt ctcgcaagcc gaataacggc tcaaactgct
     4681 ccgggaattc gttcagacgc tctgcggtat cggccatatg ttgaaaagcc aggttcataa
     4741 agcgcgtcat ctctttctct gacgcccagc gcggcacata gtcagcccca ccccacacgg
     4801 caaccagcca ctgtgccggt tcaatctctt gcggagaact caacaccgcc gtcaataaac
     4861 cgtccagctc cgccacatca aggatggcgt ggtcagtgtt gtatttggtc agaatatcgt
     4921 ccagccattc caactcactt tcgtttaacg gtcccgtttt catacgcttt tccttgtgga
     4981 tctcaactcg ccagcaccta tcttacatgc cggtccgtat cagagatact ttttgagtgg
     5041 ctttgccggt gattaaaaat caaggagggt gtaacgacaa gttgcaggca caaaaaaacc
     5101 acccgaaggt ggtttcacga cactgcttat tgctttgatt ttattcttat ctttcccatg
     5161 gtacccggag cgggacttga acccgcacag cgcgaacgcc gagggatttt aaatcccttg
     5221 tgtctaccga ttccaccatc cgggctcggg aagaaagtgg aggcgcgttc cggagtcgaa
     5281 ccggactaga cggatttgca atccgctaca taaccgcttt gttaacgcgc caaattcttc
     5341 aggcctttca gccagacatc cgctgacgcc gatgtctttt aaactggagc gggaaacgag
     5401 actcgaactc gcgaccccga ccttggcaag gtcgtgctct accaactgag ctattcccgc
     5461 attcatcaag caatcagtta atcacttgat tttattatcg tctggcaatc agtgccgccg
     5521 ttcgatgcgt tgcattctac ttacctggcg cgatgagtca acgatatttt tcaccacttt
     5581 tgatcgtttg ctgaaaattg cgccgaaacg atcactgatc aagcaaatct gcacgcgcag
     5641 cgctcaaata ttgcaacatt gaccacagag tcagtaccgc agccacaaag aaaagtgcaa
     5701 taccggcgta ctcaacccaa atgttcggac gccacagcag ccatgccaac gccaccatct
     5761 gggcagtggt tttcactttc ccaatccagg agacagccac gctactgcgt ttacccaact
     5821 ccgccatcca ttcgcgtagc gcagaaataa taatttcacg ggcgatcatc gttgccgccg
     5881 gtaaggtcac ccaccagctg tggtaatgct cggttaccag caccatggcg atagccacga
     5941 gaactttatc tgctacaggg tcaaggaaag caccaaaccg ggtactctgg ttccagcggc
     6001 gtgccagaaa accatcgaac cagtcagtca ccgccgcgac gcagaaaatg agcgcggcgg
     6061 caaacggcga ccaagtgaca ggcagataaa agaccaatac aaagaatggg ataaggatga
     6121 cacggaacag tgtaagcaac gtagggatat taaattgcat aatgacgggt aactatctgt
     6181 tgtcagtaag attaccccta tgttgctaca gagacatcaa tgtttcaacg accagaagat
     6241 cttttctgcc agaccttgcg aaatacccgg cacttttgca atttcctcga cgctggcgtt
     6301 acgtaaacct tgcaaaccgc ccatatattt caacaacatt tgccggcgtt ttggcccgac
     6361 gccttcaatg gtttccaggg aactggtatt tttgaccttc gcccgttttt tacggtgccc
     6421 gccaatcgcg tgatcgtgtg attcatcgcg aatatgctgg ataacatgca gcgcgggaga
     6481 atctggcggc aaactgaatc cctcaccttc cggctcaaag aacagcgttt ccagcccagc
     6541 cttacgatct gctcctttgg caacaccaag tagcagcgga tgatttttat cccatgagac
     6601 atccagttcg gcgaagacat ttttcgcctg cgcaagctgg cctttgccgc cgtcgataag
     6661 tatcacatcc gggatcttac tgtcgtcgat ggctttacca taacgccgac gcagcacctg
     6721 attcatcgcc gcataatcat cgcccggcgt gatgccagta atgttatagc gccgatactc
     6781 cgcacgcagc gggccgttag catcaaacac cacacaggaa gcgacggttt gttcacccat
     6841 ggtatggctg atgtcaaagc actccatccg cttcacttcc ggcaatttca acacgctggc
     6901 aagtgctgta agccgctggt gaacggtaga ttgctgcgaa agtttgctgg ttaaggccgt
     6961 cgccgcattg gtgcgcgcga gtttcaggta acgcgcccta tcgccacgag gtttggtttg
     7021 aacattaatc ttgcgtcccg ccagttctga aagggaatcg gcgagcagcg ttttatcgct
     7081 aagattaaaa tcgagcagga tctcacccgg taaggtgcgc atctggctgc cttgtaaata
     7141 gaactggcct acgaaggttt ctaccacctc gctcagttcc gtaccgccag gcactttcgg
     7201 gaaatagctg cggctgccga gcactttgcc ctgacgaatg aacaatacgt ggacacaagc
     7261 catacccgca tcgaacgcca caccaataac gtcgaggtcg tcgccggtat tggaaacgaa
     7321 ttgtttttcg gtgacgcgtc gcaccgcctg aatttggtcg cgaatacgtg ccgcttcttc
     7381 aaactccaga ttctgactgg cagtttccat acggctaatg agttgcgtaa gcacctgatc
     7441 atctttgcca gacaaaaaca ggcgcacata ctcgacctgc tgagcgtatt cttcttcact
     7501 caccagtcct tcaacgcacg gtcccagaca gcgccctatc tgatattgca gacacggacg
     7561 cgagcgattg cgataaacac tattttcgca ctggcgaatg gggaaaatct tttgcagtag
     7621 cgccagtgtt tcacgtacgg catagccatt cgggaacggg ccgaaatatt cacctttggc
     7681 atgctttgca ccacgatgca tcgccagacg cgggtgggta tcgccactca ggaagataaa
     7741 aggatatgat ttatcatcgc gtagcaaaac gttgtaacgc ggctgataga gtttgatgta
     7801 gttgtgttcc agcaacagcg cttcggtttc tgtatgagta accgttacat caatttgctg
     7861 gatctgggcg accagcgctt cggttttgcg cgaagcgagg ttgctacgga aatagctgga
     7921 aagccgtttt ttcaggtctt tcgctttgcc gacatagata accgtaccac cagcatcgta
     7981 catgcgataa acgccaggct ggctggttac ggtttttaaa aacgcttttg cgtcaaactg
     8041 atcactcact gacttgataa tgtctccgca ttacacagac catggcgaat tgccaggtga
     8101 gtcagctcaa catcgccatg aatgtttagt ttactgaaca tacgatagcg gtagctgttc
     8161 accgttttcg gactgagatt gagctgttct gagatctcat tgaccttctg gcccttcgtg
     8221 atcatcagca taatctgcaa ttcacgttca gacaaactgg caaatgggct ttctgttttt
     8281 tctggttcga tctggcttaa tgccatttgt tgagcgatgt cagaagcaat gtaacgctgc
     8341 ccagaataga cagaacggat cgcactcacg acttcctgcg gagccgcgcc tttgctgagg
     8401 tagcccgcag caccggcctg catgactttc gctggtaaag ggttttctgt atggacggta
     8461 agcatgatga tttttacatc agctgtggaa cgcgcgattt tacgcgtcgc ctcaagaccg
     8521 ccaatgcccg gcatactcat gtccattagc accacgtcaa cggcatttgc ccggcaccac
     8581 ttaacggcgt cttcaccgca cgatgcctca ccgacgactt ttataccctt tatatcttcc
     8641 agaatgcgtc gtatccctgc gcgcaccagt tcgtggtcat caacaagtag aacgttgatc
     8701 aaaggaatat ctccagaaat agggataacg ctactgatag ttagtcattc gtatattagc
     8761 ggtttttatt gcaactttga aacgttaaaa atggtgggta ttcgattttt ctctcgtttt
     8821 tgtcaattcg attgtccaca gttatggtca cgcccgtcac caacggctta ccggatcgtt
     8881 tcacagtaag ccctgcgacg aaatgtgaca aaaattacct ttattcagca aaaatgaaaa
     8941 tcagcccgct gaatatgtaa cattaattaa cccgaacaat acgggcaaaa aacattcatg
     9001 ataaaaatat ttattggtca ttatattaac gcatttaata gcaccgttga cggcacgctc
     9061 aaaaaacaac cactgctatt tttagcaaag cttatggtat actccgccgc cttaacattt
     9121 ttcaccgcaa attttcattg caacatgacg aggaaaataa atgagtacgc ctgatttttc
     9181 tactgccgag aataatcaag aactggcaaa tgaagtctcc tgcctgaaag cgatgctgac
     9241 gctgatgctg caggcgatgg gacaagctga cgcgggccgc gtgatgttaa agatggaaca
     9301 acagcttgcg ctgatcgaag acgaaaccca ggctgcagta ttttccaaaa cggttaagca
     9361 aattaaacaa gcctaccgtc agtaatataa aaccggctga tagcgtgcct ttcagccggt
     9421 ttttgcatct ggcacgcagg acagaaaaga gatcaaatta agccagtagc ggccgcgtaa
     9481 caggcaacct gggtcttatt tggtgcatta atttttttct gcatgttttt ctggtggaaa
     9541 ttgaccgtat tctcagagat tgacaaaatc atcgctatct ctgctgatgt cttcccttcc
     9601 gccgtccacc tcagaatttc tttttcgcgc ttgctgaaat tcatctctgg cgtcatcact
     9661 atttcatcat ttaaacgcat cagagccatc agactttcgc gcaccagtaa ctgcattttt
     9721 aattgcagtt catcactaag aatgggtatt tcgcgcgcgc tgcaacggga aaaggacaaa
     9781 aagcccagcg cccggtttgg cagcattaaa tactgagtga caccgcggcg taaaccatgt
     9841 gcgcgcgcgg cttcccataa cggctgtgct tcgttgaata agtcatcatt ccacattaga
     9901 tggccctgac taaagttttc agggttcagc accggatcaa ttgcgagaaa gttttttgcc
     9961 tgataataac taacccacgc ctcagggtaa ttggtgtaga aagccacttt aggtcgagtg
    10021 aatggtaccg ggtggcggac acataacgaa tagtaatcgt actccagctg ctgagcctga
    10081 agctcaattt catggtagac ctcttctgcg gtctccatcc tctgaaaacg caacagcatc
    10141 gtgcgacgcc agctgaaaaa atccgtatcc tgcatagtaa accgcaacgc ccctgagagt
    10201 gagtatcatt tataatgata attcaaacta tcacataaat aagatttata tataatttat
    10261 attattcagg caatgaatta cttttgcaag ccatcgcatt ctcttatgtt attaatgagt
    10321 tatgctgatt ttttaagcag ttttatcagg cttgaaatgg cgttccgccc cgactgtcga
    10381 atcgtcgggg ctgatttttt cttattattg cagcagaaac ttctcgagga actggcgggt
    10441 gcgaggctgc tcgggatcgg caaataacgc ttttgcggcc ccctgctcga ctatccgccc
    10501 ctggtccata aaaatcgccc ggtccgcaac atcccgggca aagctcattt cgtgtgtcac
    10561 aatcaccatc gtgcgctttt cctgcgccag ctgacggatg gtgttcagga cttcgcccac
    10621 cagctctgga tccagcgctg acgttggctc gtcaaacaaa atcacctcag gacgcattgc
    10681 cagcgcacgc gcaatcgcaa cacgctgctg ttgaccgcca gacaaacgac gtggatagct
    10741 ggtttcttta ccggccagcc caactttcgc caacagcttg cgagcgcgcg ccgtggcctc
    10801 ttctttcggt tcgcctttga cgatcaccgg cccttcaata atgttctcca gcaccgtacg
    10861 atgcggaaac aaattaaagt tctggaagac aaacccgacg tgctgacgca actggcgaat
    10921 cagcgatttt tgctgactta atgaacgtgc agtatcaata gtgatatcgc caacggtgat
    10981 cgttcccgct tccggttgtt ccagcagatt tatgctacgt agcaatgtgg ttttgccgga
    11041 accactcgga ccgataattg ccaccacttc gccaggcttt acctcgaggt cgataccgtg
    11101 cagcaccgtc tgaccgtgga attttttcac taggttctta acttcaatgg cactcatttg
    11161 ggttctctct cctggcgatt aagttgattc tcaaaatggt tctgcaacgt cgataacaca
    11221 gtcgccatga tccagtagat gagcgaagcc gccagataca tggtgaatac ctccagcgta
    11281 cgcgaggtaa tcaactgcgc ctgacggaac agctccggca cctggattgt cgcggccagc
    11341 gacgtatctt ttaccagact gataaagctg ttcgagagcg gcggcaacgc cacccgcgcc
    11401 gcctgtggca aaatcgcgcg acgcatagtc tgccatggcg tcataccaat actggcagct
    11461 gcttcccact gacctttatc aatagaagaa atggcggcgc gaagcgtttc ggcagtatag
    11521 gcggcagtgt tgagcgacag accaatcatt gctgacggaa tgggatctaa ttcgatacca
    11581 aactgcggca ggccgtaata gatcataaac agttgtgcga tgagcggcgt accacgaaaa
    11641 atagagatat aaaaacgcgc cagccagcgc acgggccaga ttggcgacag gcgcatcagc
    11701 gcgagaataa accctagcag taaaccaaaa aacatgccgc cgatactgag ttgcagcgta
    11761 tacccagccc ctttcagcag gaacggcaag gaatcaataa ccagttgtat actttcttgc
    11821 atgagcgttc ccggagctaa acgtggggat gataggcgaa cagcgcaggc gcgccgccgg
    11881 tatgaataaa cagaatcggc ccttcatctt tgaagcgttt ctgactgata ccgtcaatca
    11941 gacccgccat cgcttttccg gtatacacag gatcaagcag aatgccttcc agccgcgcca
    12001 gcaatttcac tgcttccatg ccttcgtcgt tcggcacgcc gtagccaggt gcaaaatagt
    12061 catcccagag taaaatttcc actgatgcgg tcagctccag ttctttcgca atcgcctgtt
    12121 gtaggttaac cactttcggc aattgatcgg caacggaacg cgacacggtc acgccaatca
    12181 gttcgctttc aggcatcagg tgttccagcc caacagccag tccggcgtga gttccggcac
    12241 tgcccgatgc gactaccacc gatgaaatat taaccgcccc ttcacactgt tgcgcgattt
    12301 ccagcgcact ctccacataa cctagcgcgc caagagcatt agaaccgcca accggaatga
    12361 catacgggcg aaagccttgt gcttcgactc gcgtcgccag ctcttccagt tgggcattgg
    12421 gatcggtcag tgcgtcgcac atttcaatct gggtattgaa cagatccagc aacaagcgat
    12481 tgccgttggt taaatagttt tctgcggttg tgccaatagg attttccagc agcgccacgc
    12541 agtgcagacc gagtttggca gcgactgcgg cagtctgccg cacatggtta gactggatcg
    12601 ccccggcagt aatcagcgta tcggcacctt cacgcagagc atctgccgcg agaaattcta
    12661 gcttacgtaa tttattgccg cccattgcca tgggggtgac gtcatcccgt ttgatgaaaa
    12721 tttcccgtcc tagataatca gaaaagcgcg gcagatattc gagcggcgtt ggcgcgccga
    12781 taaactccag ccgtggaaaa cgggttaaat tatgcagtgg cataacagcc tccgatgtgt
    12841 gttgttgtga ttttctcatt atgcacgctg aaaacgcgta aataaaaaag gcgctagtga
    12901 aagcgccctt ttttgtcatt atgctgatta tttggtcaca tcagcaccaa accatttttc
    12961 ggaaagggct tgcaaagtgc cgtctttttg catttccgca attgcatcat tcactgcttt
    13021 cagcaggtct tcatttcctt tacgcagcgc cacgccagac tcctgacggg agaatgcttc
    13081 accggttact gccagcgtat cgttggtttt cttcaccaga tccagcgccg ccagacgatc
    13141 aacgaggatc gcatcgatac gccctacgcg cagatcctga tatttggtcg ggtcatcatc
    13201 ataggtacgc acatcgacgc cctgaacatt ctgccgcagc cactcttcat agttggtgcc
    13261 cagaccgacc cccacttttt tgcctttcag atcatcggct gttttaatgg tgccttcgtt
    13321 accttttttc accagcgcct gaataccaga aatggtgtac ggggttgaga aatcgtattt
    13381 tttcttgcgc tcatcagaaa tggtgacctg attaatcacc acatcaatac gtttagagtc
    13441 cagcgacgcc agcataccgt cccatttggt cggttttagt gacgcctcaa cgccaagatg
    13501 ttttgccagc tgttgggcaa attccacttc aaaaccggtt aatttgccgt catccccctg
    13561 aaaactgaac ggcggataag taccttccag ccctaccagc agcgtgccgc gctctttaac
    13621 tttattaagc agaccttcat ctgcaaaact tttaacgctc atgcccgcaa tcagcgctac
    13681 ggccatcaca cccatcaatg cctgacgtcc cagatgtgct aatttcatat tcaccccgaa
    13741 tgttgttatg tctgtttgca gtgtagagcc attgtttgta aacacaaaaa caactccgct
    13801 acatcttatt cttatttaat atatatcaga agcaggcagg ctggaggatt tctgcacaag
    13861 tccggtacag gtttggcgct gatagtgttg atacttccgt aacgcgatgc ggtaattgtt
    13921 ggtgctggtg gtgggcagcc acggggcgag gatttcatca aggaaaccgt cctgcagctg
    13981 atcgagggaa atattttgct cgtgtagatg attccccaaa cggcgcagac gaacgacata
    14041 ttcacgcacc gtaccagggc tcatttcagt ttgctcaaaa agaaattgct taaagccgat
    14101 aatatcgaaa aaatcactct gcgttttgca atgtaaatca ccgcaaaatc ggcacaatgc
    14161 cgcccatgat ttttgttccg tttgccagcc attttcatca agcagcgtgt ccaggcggga
    14221 aatctcgatt ttattcacta ttttgccgtc gcgaactaag ctaatgcgat cgagcaattt
    14281 acggcaatgc gcgcaatggg tctggctgtg tttaaagtct ttaagatagc ggcttaatgg
    14341 ccgtcttttc aggtgctgca ccatcattaa gaactcctgg tagtcaaagt taaagtgcgg
    14401 catttactga cgttataact tacccagttt agtgcgtaac cgtttaatag cctggctgtg
    14461 taactgactg acccgcgatt ccccgacctc cagcactgca ccaatctctt tgagattcag
    14521 ctcttcctgg taatagaggg ttaataccag tttttcgcgc tccggcaacg tttcgatagc
    14581 ttccatcacc cgctggcgca gattactgtc cagtagttgt tgtagcgggt tttctcgttg
    14641 atgatcatca gtaaccaatt cgatgctatc gccgtgctct tcgcgccact catcgtagga
    14701 gaagagctgg ctgttattgg tgtcgagcaa catttggcga taatcggcaa tatcgatccc
    14761 taaacgttcc gctacctcag tttccgtggc gttgcggcca agttcctgct ccagttgccc
    14821 tattgcctgt gccacttcac gcgcgttgcg tcgcacgctg cgcggcaccc agtcacggct
    14881 gcgaagttca tccagcatag cgccacggat acgctgcact gcgtaagttg taaatgccgt
    14941 tccttgtagg gcgtcatagc gttcgacggc attaagtaac ccaatgccgc ccgcctgtag
    15001 cagatcgtca agttccacgc tcgcgggcag tcgaacctgc aggcgcaatg cttcgtgacg
    15061 caccagcggg acataacgct gccacagcga gtgtttatcc attacacctt cagcggtata
    15121 gagtgaattc acgataaaca gccctgcgtt atatgagtta tcggcatgat tatccgtttc
    15181 tgcagggttt ttaatcggac gattagtggg tgaaatgagg ggttatttgg gggttacagg
    15241 taaattccag gcagaaaaaa ccccgccggt ggcggggaag cacgttgctg acaaattgcg
    15301 ctttatgttg ccggatgcgg cgtaaacgcc ttatccggcc tacaagaatg tgcaaattca
    15361 ataaattgca attcaacttg taggcctgat aagcgcagcg catcaggcaa tttggcgttg
    15421 ccgtcagtct cagttaatca ggttacaacg attaaccctg cagcagagac agaacctgct
    15481 gcggtacctg gttagctttt gccagcacgg agttaccggc ctgctgaatg atctgcgctt
    15541 tcgacatgtt ggacacttcg gtcgcatagt cggcgtcctg aatacgggac tgcgcttcgg
    15601 acaggttggt agtggtgttg ttcaggttgg tgactgcgga atccagacgg ttctggatag
    15661 cacccaggga agaacggaat ttgtcgatgg agctgattgc gtcgtccagg gcagcaagcg
    15721 ggttggtcgt tgcagaacca gcactggtag tctcagtagt gattttaccc gcggagttca
    15781 catttacagc agtaccaatt gctggagcat aatctttatt ggtatcagtc gctgaagcat
    15841 acccggcaac agtcacagag ccgttatcct tgttaacgct gtaagagact gtatagtcgg
    15901 caacgttagt gataccacct ttatcatcca cataagactt cgcagcatca ctggattcac
    15961 ccgcggtaaa cccaatagtt ttgctcagta caccggaatt aaaggtaatt gaagatgtcg
    16021 cggcagccgc tttggtttcg ctcaatacta catctttact cactgtagcc tgataagtaa
    16081 tcccaccagg gattaacgga gctactggag tggttgtcgc aggagttgct tttgcgatgg
    16141 tatattctgt gccattgaat gtcagagagg caccgtcact accttcgctc gctgctttga
    16201 gcagcgcttt catatcagct ttagctgcgc taccagcgtt gttagtcgtc aagttgcctg
    16261 catcgtctac gtatgcctgg cttccaccga tggtgatatt acctgctgaa tccgtttcga
    16321 aagaaacagt accatctttt gtggtgtaag aaccattaac agatttaccc acatcagcat
    16381 gaagggtggc agctaacgca tcacgttttg aagctgaatc agcagcctgc gccgcggcgt
    16441 ctacacccgt accagcagta gatttagtgg tagtaaaatc accagattta gcgttgtaag
    16501 tataatctac gccgccaact gtgactttat cgccattccc taatttatcg aatgcagcat
    16561 cggtagttaa caaggtattt tcggttttca gatcataaag acctgtcgtg gtgtttaact
    16621 tcgcgccagc agaagttaaa tcacttaccg ttgcagcttt gttggtaata gtacctttac
    16681 catttacgtt aaagccattc agacccagag tatcagaatc gatttttttc aggtcgatcg
    16741 tgatggtttc gccgtcattc gcaccaacct gaattttcat tgaaccgtct ttcgccagca
    16801 cgttcacgcc gttgaactgg gtctggccgg atacgcggtc aatttcatca agacgagatt
    16861 tgatttcgtc ctggatggag tccaggtcag aatcggagtt agtccctgta gtggcctgaa
    16921 ccgtcagttc acgaatacgc tgtaagttgt tgttgatttc ggacagcgcg ccttcggtgg
    16981 tctgcgcaac agaaataccg tcgttggcgt tacgggccgc ctgagtcagg cctttaatgt
    17041 tagaagtaaa acggttagca atcgcctgac ctgcggcgtc atccttcgcg ctgttaatac
    17101 gcaagccaga agacagacgc tcgatagaac tcgacagcgc agactggttc ttgttgatat
    17161 tattttgagt gatcagcgag aggctgttgg tattaatgac ttgtgccatg attcgttatc
    17221 ctatattgca agtcgttgat tacgtattgg gtttccaccc gtcggctcaa tcgccgtcaa
    17281 acctgttatc gtctgtcgta aaacaacctt tagaattttt ttcacaaaca gccatttttt
    17341 gttagtcgcc gaaatactct tttctctgcc ccttattccc gctattaaaa aaaacaatta
    17401 aacgtaaact ttgcgcaatt caggccgata accccggtat tcgttttacg tgtcgaatga
    17461 taaaaggaaa tcgcatggca agtatttcat cgctgggagt cgggtcaggt ctggatttaa
    17521 gttcaatcct tgatagcctc accgccgcgc aaaaagcgac gctaaccccc atttcaaatc
    17581 agcaatcgtc gtttaccgct aaacttagcg cctacggtac gctgaaaagc gcgctgacga
    17641 ctttccagac cgccaatact gcattgtcta aagccgatct tttttccgcc accagcagca
    17701 ccaccgcgtt cagtgccacc accgcgggta acgccatcgc cgggaaatac accatcagcg
    17761 tcacccatct ggcgcaggcg caaaccctga ccacgcgcac caccagagac gatacgaaaa
    17821 cggcgatcgc caccagcgac agtaaactca ccattcaaca aggcggcgac aaagatccga
    17881 ttaccattga tatcagcgcg gctaactcgt cgttaagcgg gatccgtgat gccatcaaca
    17941 acgcaaaagc aggtgttagc gcgagcatta ttaacgtggg taacggtgat tatcgtctgt
    18001 cagtcacatc aaatgacacc ggccttgaca atgcgatgac gctctcggtt agcggtgatg
    18061 atgcgctaca aagttttatg ggctatgacg ccagtgccag cagcaacggc atggaggtct
    18121 cggtcgccgc ccagaatgcg cagctgacgg tcaacaacgt cgccattgag aacagcagca
    18181 acaccatcag cgacgcgctg gaaaacatca ccctgaacct gaacgatgtc accacgggca
    18241 accagacgct aaccatcact caggacacct ccaaagcgca aacggcgatt aaagattggg
    18301 tgaatgccta taactcgcta atagatacct tcagcagcct gaccaaatac accgccgtag
    18361 atgcgggagc tgatagccag agttctagca atggcgcact gctcggcgac tccacgctgc
    18421 ggacgattca gacgcagttg aagtcgatgc tgagtaatac cgtcagttct tccagctata
    18481 aaacgttggc gcagattggt atcacgaccg atcccagcga tggcaaactg gaactggatg
    18541 ccgacaaact caccgctgca ctgaaaaaag atgccagcgg cgtaggtgca ttgattgttg
    18601 gcgatggtaa aaaaaccggc atcacgacca ccatcggcag caacctgacc agttggcttt
    18661 cgacaacggg cattattaaa gccgctaccg atggcgttag taagaccctg aataaattaa
    18721 ctaaagacta caacgccgcc agcgatcgca ttgacgcgca ggtcgcgcgc tacaaagaac
    18781 aatttaccca actggacgtt ttaatgacct cgttaaacag cacaagcagc tacttaacgc
    18841 agcagttcga aaacaacagt aattccaagt aatcattatt catcgggaga caggtcatgt
    18901 acgcggcaaa aggcacccaa gcctatgcac aaattggcgt cgaaagcgcc gtaatgagcg
    18961 ccagccagca gcagctggtc accatgctat ttgatggagt gctgagcgca ctggttagag
    19021 cgcgcctgtt tatgcaggac aacaatcagc aaggcaaagg cgtctctttg tcaaaagcga
    19081 tcaacatcat tgagaacgga ctgcgggtga gtcttgatga agagagcaaa gacgaactaa
    19141 cccaaaactt gattgctctt tatagctata tggtcaggcg tttgctgcaa gccaatttac
    19201 gcaacgatgt ctccgcagtc gaagaagtgg aaacattaat gcgcaatatt gccgatgcct
    19261 ggaaagagtc gttactctcc ccttctttga ttcaggaccc agtctgatga accatgcacc
    19321 gcatttatat ttcgcctggc aacaactcgt cgaaaaaagc cagctcatgt tacgcctggc
    19381 aacggaagaa caatgggacg aactcatcgc cagcgaaatg gcgtatgtta atgcggtgca
    19441 ggagattgca catttgactg aagaggttga cccgtccacc acgatgcagg agcagctccg
    19501 cccgatgctg cgcctgattc tcgacaacga aagcaaggta aagcagttat tacagattcg
    19561 gatggatgaa ctggcgaaac tggtcggtca gtcatcggtg caaaaatcgg tgttgagtgc
    19621 ctatggcgat cagggcggct ttgtgctggc tccgcaggat aacctctttt gaatctgaat
    19681 gagtcgatgg ctcgcgaata atccgattac ggctacgctt ctaatgttcc ccttgaatgg
    19741 agtcgaagaa tgcgtaatcc cacgctgtta caatgttttc actggtatta cccggaaggc
    19801 ggtaagctct ggcctgaatt ggccgagcgc gccgacggtt ttaatgatat tggtatcaat
    19861 atggtctggt tgccgcccgc ctataaaggc gcatcgggcg ggtattcggt cggctacgac
    19921 tcctatgatt tatttgattt aggtgagttt gatcagaaag gcagcatccc tactaaatat
    19981 ggcgataaag tacaactgct ggccgccatt gacgccctga aacgtaatga cattgcggtg
    20041 ctgttggacg tggtagtcaa ccacaaaatg ggcgcggatg aaaaagaagc tattcgcgtg
    20101 cagcgcgtta atgctgatga ccgtacgcaa attgatgaag aaatcattga atgcgaaggc
    20161 tggacgcgtt acaccttccc cgctcgtgcc gggcaatact cacagtttat ctgggatttc
    20221 aaatgtttta gcggtatcga ccatatcgaa aaccccaatg aagatggcat ttttaaaatt
    20281 gttaacgact acaccggcga aggctggaac gatcaggttg atgatgaact cggtaatttc
    20341 gattatctga tgggcgagaa tatcgatttt cgcaatcatg ccgtgacgga agagattaaa
    20401 tactgggcgc gctgggtgat ggaacaaacg caatgcgacg gttttcgtct tgatgcggtc
    20461 aaacatattc cagcctggtt ttataaagag tggatcgaac acgtacagga agttgcgcca
    20521 aagccgctgt ttattgtggc ggagtactgg tcgcatgaag ttgataagct gcaaacgtat
    20581 attgatcagg tggaaggcaa aaccatgctg tttgatgcgc cgctgcagat gaaattccat
    20641 gaagcatcgc gcatgggacg caactacgac atgacgcaga ttttcactgg gacgctggtg
    20701 gaagccgatc cttttcacgc cgtaaccctc gttgccaacc acgacaccca accgttgcaa
    20761 gccctcgaag caccggtcga accgtggttt aaaccgctgg cgtatgctct aattttgttg
    20821 cgggaaaatg gcgttccttc ggtgttctat ccggacctct acggtgcgca ttacgaagat
    20881 gtcggtggtg acgggcaaac ctatccgata gatatgccaa taatcgaaca gcttgatgag
    20941 ttaattctcg cccgtcagcg tttcgcccac ggtgtacaga cgttattttt cgaccatccg
    21001 aactgcattg cctttagccg cagtggcacc gacgaatatc ccggctgcgt ggtggtcatg
    21061 tcgaacggag atgatggcga aaaaaccatt catttaggag agaattacgg caataaaacc
    21121 tggcgtgatt ttcttgggaa ccggcaagag agtgtagtaa ccgacgaaaa cggcgaagcg
    21181 accttctttt gcaacggcgg cagcgttagc gtgtgggtta tcgaagaggt gctttaaatt
    21241 catccccggc ggcaagccgg ggagatttca ttacggcaat ggcgtcggta acggcgcttt
    21301 atccagcgca gcggcacact ctgccgtcgg gcgatccaca cgctccatcg tcataccatc
    21361 gtactcaatg gtgttaccgt cacgctcaac ctcgtacagc tcacgtttta cggtaacgtt
    21421 agtgagatca tcagacatca atgtcaactt gcccggcaca gcaatcaccc gctgccactg
    21481 acggcaatct agggtatcgc cctctttagt gacaatcagg ctgcctatcg cttccgggct
    21541 gaccagcgca cgttgcggcc ctttggtctg ccagtagccc gccagccagt ccggcgcggg
    21601 ggttttcacg acattgttat agttttcgac ttcggcacag cccgccagca acatgagcgc
    21661 gcctgcaatt gcgagttttt tcatcattcg tcctgcatga gaagaaaaga ggattgtggc
    21721 attaaagccc tgatgtcgcc agcctttcca ggttcagaga gaaatttgat ctaactatta
    21781 tttttgtgaa ttattacccc gaaaataaat ttgcatggtg tatgattcgc gactcttgtt
    21841 tcaaaccttc tgagttcaga ggctacattc atgtcatggc agcaattcaa acacgcctgg
    21901 ttgattaaat tctgggcgcc catccccgcg gtcatcgcgg cgggtattct ctccacttac
    21961 tattttggca tcactggcac cttttgggct gtcacgggtg aatttacccg ttggggcggt
    22021 cagctcctgc agctgttcgg cgtccatgct gaagagtggg gttattttaa aattatccat
    22081 ctggaaggat cgccattaac ccgcatcgac gggatgatga tcctcggtat gtttggcggc
    22141 tgctttgccg cagcgctgtg ggccaacaat gtcaaactac gcatgccgcg tagccgtatc
    22201 cgcattatgc aggccattat tggcggcatt atcgccggtt ttggcgcgcg tctggcaatg
    22261 ggctgtaacc tggcggcatt ctttaccggt attcctcagt tctcgctgca tgcctggttc
    22321 tttgccatcg ccactgccat tggttcatgg tttggcgcgc gctttaccct gctgcccatc
    22381 ttccgtattc ccgtgaaaat gcagaaagtt gctgccgcct caccgctgac gcaaaaaccg
    22441 gatcaggcgc ggcgtcgttt tcgtctcggg atgctggtct ttttcggcat gctgggctgg
    22501 gcgctgctca cagcgatgaa ccaacccaaa ctggggctgg caatgctgtt tggcgtcggc
    22561 tttggtttac tgattgaacg tgcgcaaatc tgctttactt cggcgttccg cgatatgtgg
    22621 atcaccggac gtacccatat ggcgaaagca atcattattg gtatggcggt gagtgccatc
    22681 gggatcttca gttacgtaca gttaggcgtt gaacccaaaa tcatgtgggc gggaccaaac
    22741 gcggtaattg gtggtttact gtttggtttt ggcatcgtgc tggctggcgg ctgcgaaacc
    22801 ggctggatgt accgcgcggt agaaggccag gtgcactact ggtgggtcgg tctgggcaat
    22861 gtgatcggct caacgattct ggcgtattac tgggatgatt tcgctccggc gctggccacc
    22921 gactgggaca aaatcaactt gctgaaaacc tttggtccga tggggggcct gctggtgaca
    22981 tatttgctgt tgtttgctgc gctgatgttg attatcggct gggaaaaacg cttcttccgc
    23041 cgtgcggcac cgcagactgc taaggagatc gcatgaaaaa tatcgttcct gattaccgcc
    23101 ttgatatggt gggtgaaccc tgcccttatc cggcagtcgc aacccttgag gcgatgccgc
    23161 agttaaaaaa aggggaaatc ctggaagtgg tgagcgactg tccgcagtcg atcaataata
    23221 ttccactgga tgcgcgtaat cacggctata cggtgctgga tattcagcaa gacgggccga
    23281 ccattcgtta tttaattcag aagtaatccc tcattcccgc tgtgacccac agcgggaaca
    23341 tcgttacctt tgtatcacat agattttacc cgccccctct cctattcttt tcagtatgat
    23401 ctcaggaggt ttctatgtgt ggacgctttg cccaatccca aacgcgtgaa gattaccttg
    23461 cgcttctcgc ggaagatatt gaacgcgata ttccctatga tcccgaaccc attggcagat
    23521 acaacgtcgc gccgggaacc aaagtcctac tgctcagtga acgtgatgaa caccttcatc
    23581 tggatccggt tttctgggga tatgctcccg gatggtggga taaaccgccg ctgattaacg
    23641 cccgcgtaga aactgcggca accagtcgta tgtttaaacc gctctggcaa catggtcggg
    23701 caatctgttt tgccgatggc tggtttgagt ggaaaaaaga aggcgacaaa aagcagcctt
    23761 attttatcta tcgtgctgat ggacaacctg tttttatggc agcgataggc agcacaccgt
    23821 tcgagcgcgg tgacgaagcc gaaggatttt tgattgtcac tgcagcagca gaccagggtc
    23881 tggtagatat tcatgaccgc cgcccgctgg ttctgtcgcc agaagctgcg cgggaatgga
    23941 tgcggcaaga gattagcggt aaagaagcct cagaaatcgc ggccagtggc tgtgttccag
    24001 caaaccagtt ctcctggcac ccggtatcgc gcgcggtggg taatgttaaa aaccagggtg
    24061 cggagttaat tcaacctgtt tgataagcct ggagattatt gatcaacaat actgcgtcat
    24121 aagtaatctc tattagacaa agatttcatt acctgttggc atattgcaaa aataacacca
    24181 atacggaatc gtcatgttca cgattaaaac agatgatctc acccatccag cagtgcaagc
    24241 attagtggct taccatattt ccggcatgct gcagcagtct ccccctgaaa gcagtcatgc
    24301 tttagacgtg caaaaattac gtaacccgac agtgacattc tggtcagtat gggaaggcga
    24361 acaactcgca ggaattggtg cgctgaagtt gctggatgat aaacatggcg aactgaaatc
    24421 aatgcggacc gcgccaaatt atttacgtcg gggtgtcgcc agtctgattt tacgccacat
    24481 tttgcaggtc gcccatgaca gatgccttca tcgcctgagt ttagaaacgg gtacacaggc
    24541 tggatttacg gcctgccatc aactttattt gaagcatggt ttcgttgatt gcgaaccgtt
    24601 tgccgattat caacttgatc cacacagtcg atttttgtca ttgacgctat gcgaagataa
    24661 tgagttgctt tgagccagac gcagcacatt cttgcattcg acgtgctgcg tctttattta
    24721 tcaccaacag gaaacgcctt gtccatagac gccccttcca catgcgtcac aagaaacctc
    24781 tattccagtg acacaattac gcctaattaa ttacatataa tatttaatta tgaattcctc
    24841 accatctatt acatgctttt taa
//
LOCUS       DAZKIP010000062        24807 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZKIP010000062 DAZKIP010000000
VERSION     DAZKIP010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.062"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(259..2832)
                     /gene="clpB"
                     /locus_tag="ACHOUK_002754"
     CDS             complement(259..2832)
                     /gene="clpB"
                     /locus_tag="ACHOUK_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFP9898168.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2962..3693)
                     /gene="yfiH"
                     /locus_tag="ACHOUK_002755"
     CDS             complement(2962..3693)
                     /gene="yfiH"
                     /locus_tag="ACHOUK_002755"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFP9898169.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLNLTGEPYASKRADASYSNTPGRVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAEASTAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3690..4670)
                     /gene="rluD"
                     /locus_tag="ACHOUK_002756"
     CDS             complement(3690..4670)
                     /gene="rluD"
                     /locus_tag="ACHOUK_002756"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFP9898170.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKLCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4805..5542
                     /gene="bamD"
                     /locus_tag="ACHOUK_002757"
     CDS             4805..5542
                     /gene="bamD"
                     /locus_tag="ACHOUK_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFP9898171.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5813..6154
                     /gene="raiA"
                     /locus_tag="ACHOUK_002758"
     CDS             5813..6154
                     /gene="raiA"
                     /locus_tag="ACHOUK_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFP9898172.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6257..6382
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6258..6305
                     /gene="pheL"
                     /locus_tag="ACHOUK_002759"
     CDS             6258..6305
                     /gene="pheL"
                     /locus_tag="ACHOUK_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFP9898173.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6404..7564
                     /gene="pheA"
                     /locus_tag="ACHOUK_002760"
     CDS             6404..7564
                     /gene="pheA"
                     /locus_tag="ACHOUK_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFP9898174.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7607..8728)
                     /gene="tyrA"
                     /locus_tag="ACHOUK_002761"
     CDS             complement(7607..8728)
                     /gene="tyrA"
                     /locus_tag="ACHOUK_002761"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFP9898175.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPALRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8739..9809)
                     /gene="aroF"
                     /locus_tag="ACHOUK_002762"
     CDS             complement(8739..9809)
                     /gene="aroF"
                     /locus_tag="ACHOUK_002762"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFP9898176.1"
                     /translation="MQKDALNNVHITDEQVLMTPAQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            10019..10384
                     /gene="yfiL"
                     /locus_tag="ACHOUK_002763"
     CDS             10019..10384
                     /gene="yfiL"
                     /locus_tag="ACHOUK_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFP9898177.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASAIRLN"
     gene            10534..11052
                     /gene="yfiR"
                     /locus_tag="ACHOUK_002764"
     CDS             10534..11052
                     /gene="yfiR"
                     /locus_tag="ACHOUK_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFP9898178.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            11042..12268
                     /gene="dgcN"
                     /locus_tag="ACHOUK_002765"
     CDS             11042..12268
                     /gene="dgcN"
                     /locus_tag="ACHOUK_002765"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFP9898179.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLP
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSAA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYGVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIAHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            12284..12766
                     /gene="yfiB"
                     /locus_tag="ACHOUK_002766"
     CDS             12284..12766
                     /gene="yfiB"
                     /locus_tag="ACHOUK_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFP9898180.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(12843..13190)
                     /gene="rplS"
                     /locus_tag="ACHOUK_002767"
     CDS             complement(12843..13190)
                     /gene="rplS"
                     /locus_tag="ACHOUK_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFP9898181.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(13232..13999)
                     /gene="trmD"
                     /locus_tag="ACHOUK_002768"
     CDS             complement(13232..13999)
                     /gene="trmD"
                     /locus_tag="ACHOUK_002768"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFP9898182.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(14030..14578)
                     /gene="rimM"
                     /locus_tag="ACHOUK_002769"
     CDS             complement(14030..14578)
                     /gene="rimM"
                     /locus_tag="ACHOUK_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFP9898183.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(14597..14845)
                     /gene="rpsP"
                     /locus_tag="ACHOUK_002770"
     CDS             complement(14597..14845)
                     /gene="rpsP"
                     /locus_tag="ACHOUK_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFP9898184.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(14982..16343)
                     /gene="ffh"
                     /locus_tag="ACHOUK_002771"
     CDS             complement(14982..16343)
                     /gene="ffh"
                     /locus_tag="ACHOUK_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFP9898185.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            16510..17301
                     /gene="ypjD"
                     /locus_tag="ACHOUK_002772"
     CDS             16510..17301
                     /gene="ypjD"
                     /locus_tag="ACHOUK_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFP9898186.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            17323..18609
                     /gene="yfjD"
                     /locus_tag="ACHOUK_002773"
     CDS             17323..18609
                     /gene="yfjD"
                     /locus_tag="ACHOUK_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFP9898187.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVVGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(18664..19257)
                     /gene="grpE"
                     /locus_tag="ACHOUK_002774"
     CDS             complement(18664..19257)
                     /gene="grpE"
                     /locus_tag="ACHOUK_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFP9898188.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            19380..20258
                     /gene="nadK"
                     /locus_tag="ACHOUK_002775"
     CDS             19380..20258
                     /gene="nadK"
                     /locus_tag="ACHOUK_002775"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFP9898189.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGATRTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            20344..22005
                     /gene="recN"
                     /locus_tag="ACHOUK_002776"
     CDS             20344..22005
                     /gene="recN"
                     /locus_tag="ACHOUK_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFP9898190.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IVEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPETLPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQHYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKTRLQELARLLGGSEVTRNTLANAKELLAA"
     gene            22154..22495
                     /gene="bamE"
                     /locus_tag="ACHOUK_002777"
     CDS             22154..22495
                     /gene="bamE"
                     /locus_tag="ACHOUK_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFP9898191.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(22557..22847)
                     /gene="yfjF"
                     /locus_tag="ACHOUK_002778"
     CDS             complement(22557..22847)
                     /gene="yfjF"
                     /locus_tag="ACHOUK_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFP9898192.1"
                     /translation="MPGKIAVEVAYALPKKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTENKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(22837..23274)
                     /gene="ratA"
                     /locus_tag="ACHOUK_002779"
     CDS             complement(22837..23274)
                     /gene="ratA"
                     /locus_tag="ACHOUK_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFP9898193.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            23445..23927
                     /gene="smpB"
                     /locus_tag="ACHOUK_002780"
     CDS             23445..23927
                     /gene="smpB"
                     /locus_tag="ACHOUK_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFP9898194.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            24142..24504
                     /gene="ssrA"
                     /locus_tag="ACHOUK_002781"
     tmRNA           24142..24504
                     /gene="ssrA"
                     /locus_tag="ACHOUK_002781"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         6319 a         6538 c         5818 g         6132 t
ORIGIN      
        1 atacgctttc ccgctacaga gtcaagcatt tatttttgct ttctctgccg gaattctcag
       61 gagaaccccg ctgacccggc ggcgtgtttg ccgttgttcc gtgtcagtgg tggcgcatta
      121 tagggagtta ttccggcctg acaagcgaaa aatataaaaa ctttatcgtt cgctcatttt
      181 tcaggcaaaa catcttaaat atagtctttt ccgtctaact tatagacaaa aacgagcccc
      241 gaagggctcg ttttatcatt actggacggc gacaatccgg tcttcattaa cttccaggcg
      301 aataacttta cccggaacca attcaccaga cagtatttgc tgtgccaacg ggttttcgat
      361 ctgctgctgg atagcacgtt tcaaaggacg tgcaccatag actggatcgt aaccgttctc
      421 gctcagcagt ttcagcgcct cgtcagaaat gtggatttca taaccacgtt cttccagacg
      481 tttgtacaga cgtttcaact gaatctgcgc aatcgaggca atgtgctgtt cgcccagcgg
      541 atggaagacc accacttcat cgatacggtt aatgaactcc ggacggaagt tatggcttac
      601 cacaccgagc accagctctt tcatgtgcgc ataatccagt tcaccgaagc gttcctgaat
      661 cagatcggaa ccgaggttag aggtcataat gacgaccgta ttacggaagt cgaccgttct
      721 cccttgcccg tcagtcagac gcccatcatc cagtacctgc aacagaatgt tgaagacatc
      781 cggatgcgct ttttccactt catccagcag gatgacggaa tacggacgac gacgcaccgc
      841 ttcggtcagg tagccacctt cttcataacc gacatatccc ggaggcgcac caaccagacg
      901 agacaccgag tgtttctcca taaactcgga catatcgata cggaccatcg cctcgtcgct
      961 atcaaacata aagttcgcca gcgccttaca aagctctgtt ttccccacac cagttgggcc
     1021 gaggaacagg aatgaaccaa tcgggcgatt tggatccgcc agccccgcac ggctacgacg
     1081 aatagcgtta gataccgcat caaccgcttc gttctgacca attacgcgat ggtgcagttc
     1141 ttgctccata cgcagcagtt tttcgcgctc gctttccatc atgcgagaaa ccggaatccc
     1201 cgtccaacgc gccagcactt cagcaatttc ggcgtcggtc actttattac gcaacagacg
     1261 catagttttg ccttcaagct gcgttgcggc ttccagttgc ttttccagtt ccgggatttt
     1321 gccgtattgc agttcagaca tccgcgccaa gtcacccaca cggcgagcct gttcaatagc
     1381 gattttcgcc tgttccagtt ccgctttaat ggtctgcgta ccagaaagcg atgccttctc
     1441 tgctttccac tcttcttcta actcggagta ctgacgttct ttgtcgctca gttcttcgtt
     1501 gagcatatcc agacgttttt tactggcttc atcagactct ttcattaacg cctgttgttc
     1561 cagtttgagc tggatgatac ggcgatcgag tcggtcgagt tcttctggtt ttgagtcaat
     1621 ctgcatacga atgctggatg ctgcttcatc gatcaggtcg atggctttat ccggcagctg
     1681 acggtcagca atgtagcgat gagacaacgt cgccgctgca acaattgccg ggtcagtaat
     1741 ttgcacatgg tggtgcaatt cgtaacgttc tttcaggcca cgcagaatcg caatggtatc
     1801 ttcaacagaa ggctcggcaa caaacacttt ctggaaacga cgttccagcg cagcatcttt
     1861 ttcgatgtac tggcgatatt cgtcaagcgt cgtggcacct acgcagtgca attcaccacg
     1921 cgccagcgcc ggtttcagca tgtttccggc gtccattgcg ccatcggctt tacccgcgcc
     1981 gaccatggta tgtaattcgt cgataaacag gatgacgttg ccttcctgtt tggcaagatc
     2041 gttaagcacg ccttttaaac gttcttcaaa ctcaccgcga tatttcgccc cagccaccag
     2101 cgcgcccata tccagcgcca gtacccggcg gcctttcaac ccttccggca cttcgccgtt
     2161 gataatacgc tgcgccagac cttcaacgat ggcagtttta ccgacgccgg gttcaccaat
     2221 cagtaccggg ttatttttag tacgacgttg cagcacctga atggtacggc gaatttcttc
     2281 atcacgacca atcaccggat cgagtttgcc ctgttcggct cgttcggtaa ggtcgatggt
     2341 atattttttc aaagcctgac gttggtcttc agcaccttga tcgttcacgc tttcacctcc
     2401 acgcatttgt tcaatcgctt gagtaatgtt ggcggtggtc gcccctgctg ctttcaggat
     2461 gtcggccagc gtgccgcgag actcaagtgc cgccagaacg aacagttctg acgagataaa
     2521 gttatcacca cgtttttgcg ccagcttgtc gcaaagatta agaacgcgca ccagatcctg
     2581 tgatggctgg acatcaccac cagtaccttc aacctgcggt aaacgattta atgcctgatt
     2641 gatatctgtg cgcaactggc cagcatttat gccagcggat gttaataaag gactaaccga
     2701 acccccttcc tgattcagca gggcgctcat taaatgaagt ggttcgataa attggttgtc
     2761 gtgcccaagt gcaagtgatt gggcatcggc aagagcaagc tggaatttat tagtaagacg
     2821 atccagacgc ataactcctc ccataacgga tcaaagttgc tactggagat taaatgaggt
     2881 catccctcaa ttattcaagg ttattgacca gattaatgtg aaaagaaaat cacgcgtacc
     2941 ggatcgtctt gattctttag gttatatcag ccaaatgaaa cttgccatac gaccggtggt
     3001 cttgtcgcga cgataagaga agaaagtctc attttccgta tatgtacaac ggtcgccgcc
     3061 gaaaatttgc tcaacaccca cgttcgccag acgctgccgg gcaagctgat aaatgtccgc
     3121 cagatactta tcaccatgct gaatgaaagc tgtactcgct tcggcgtcta ctgccataaa
     3181 cgcctcgcga acctccgccc ccacttcaaa cgcgcgtggg ccaattgccg gccctaacca
     3241 ggcgagaata ttttctggat tatcagcaaa acaggaaact gtctcttcca gcacgcctgc
     3301 gcacagtcca cgccagccag catgagcggc ggcgacttcc gttcccgctc gattgcaaaa
     3361 cagcacaggg aggcagtcgg cagtcatcac tgcgcaaacc ctgccgggcg tattgctata
     3421 agaggcatct gctcgttttg aggcataagg ttcgccagta agattaagca catctttgcc
     3481 gtgtacctgc tcaagccaga ccggtttaga aggcaaattg cccgcagcaa aaagtcgctt
     3541 gcgattctcc tcaacgtgat ccgggttatc gccacaatgg gcaccgaggt tgagcgagtc
     3601 atacgggggc aagctcacgc cgccgatacg agtagagcta caggccgcaa caccttttgg
     3661 cagcggccac tgcgggacaa tcagcttact cataaccagt ccacttcatc tttatgctct
     3721 tcgaaatcgg cgcgcatcac ctcaatcagc tccaccatat cttgtggaat aggcgcatgc
     3781 cattccattt cgataccgga gatcgggtga taaagacgca gcatggttgc atgaagcgcc
     3841 tggcggtcaa atttacgcag cgtggagata aacgcttccg aagcaccttt tggcgggcgc
     3901 ggacggccac cataaaccgg atcgcccacc agcggatgag tgatatgggc catatgcacg
     3961 cggatctggt gcgtacgtcc agtttccaga cgcaaccgca gacgcgtgtg cacacggaag
     4021 tgttccatga tgcgatagtg agtcaccgcc ggtttgccca tcggatgcac cgccatgtga
     4081 gtacgtttgg tcgggtggcg gctgattggt tcatctactg taccgccagc ggtcatatga
     4141 ccaattgcca ccgcttcata ctcacgagta atttcacgcc tttgcaaaga ttcgactaaa
     4201 cgcgtctgag ccggaacggt ttttgccaca accatcaggc cagtggtgtc tttatccaga
     4261 cgatggacga tgcctgcacg cggtacatca gcaatgggcg gatagtaatg aagtaacgca
     4321 ttcagtaccg tgccatccgg gttacccgcg ccaggatgta ccaccagatc gcgcggttta
     4381 ttaatgacga tgatatcttc atcttcatag acgatatcca gcgggatatc ctgcggttca
     4441 aaacgcgctt cttcttcaat ctcagcgttg atggcaacct gctcgccacc caatactttt
     4501 tctttcggct tatcacaaag tttgccgtta accagcacgc gctggtcgag gatccattct
     4561 tttattcgcg aacgtgaata atccgggaac atttcggcca aagcctgatc taagcgttga
     4621 ccgagttggt tttcggacac cgttgcagtg agctgtactc gttgtgccat atatactgct
     4681 tcttcgttta tcgttggggt tttacggctt tgccgtttaa tatagtgtgc tattgtagct
     4741 ggtcttaacc gggagcagga acagagaatc tcccgtatta cattttgagg aaagtcaaaa
     4801 cgtcatgacg cgcatgaaat atctggtggc agccgccaca ctaagcctgt ttttggcggg
     4861 ttgctcgggg tcaaaggaag aagtacctga taatccgcca aatgaaattt acgcgactgc
     4921 acaacaaaag ctgcaggacg gtaactggag acaggcaata acgcaactgg aagcgttaga
     4981 taatcgctat ccgtttggtc cgtattcgca gcaggtgcag ctggatctca tctacgccta
     5041 ctataaaaac gccgatttgc cgttagcgca ggctgccatc gatcgtttta ttcgccttaa
     5101 cccgacccat ccgaatatcg attatgtcat gtacatgcgt ggcctgacca atatggcgct
     5161 ggatgacagt gcgctgcaag ggttctttgg cgttgaccgt agcgatcgcg atcctcaaca
     5221 tgcacgagct gcgtttagtg acttttccaa actggtgcgc ggctatccaa acagtcagta
     5281 caccaccgat gccaccaaac gtctggtatt cctgaaagat cgtctggcga aatatgaata
     5341 ctccgtggcc gagtactata cagaacgtgg cgcatgggtt gccgtcgtta accgcgtaga
     5401 aggcatgttg cgcgactacc cggataccca ggctacgcgt gatgcgctgc cgctgatgga
     5461 aaatgcatac cgtcagatgc agatgaatgc gcaagctgaa aaagtagcga aaatcatcgc
     5521 cgcaaacagc agcaatacat aacagaaacc tgaaacacaa aacggcagcc cttgagctgc
     5581 cgtttgttta ttctgtcagt tgtgaaactg aagcgattta gtcactatcg atctcatcaa
     5641 atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc gatttctcac
     5701 aaaaaagatt cgttgacaaa aagtgacaaa attatgagat ttccatcaca cattttgaca
     5761 tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat ttatgacaat
     5821 gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg tcgcagaccg
     5881 tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca ttctgtccaa
     5941 agagccacaa gggtttgttg ctgacgccac aatcaataca cctaacggcg ttctggttgc
     6001 cagtggtaaa catgaagata tgtacaccgc aattaacgaa ttgatcaaca agctggaacg
     6061 gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat cggtgaaaga
     6121 cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg tatcgccaac
     6181 gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacgggtact gtactaaagt
     6241 cacttaagga aacaaacatg aaacacacac cgtttttctt cgcattcttt tttaccttcc
     6301 cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agaagaacaa taaggcctcc
     6361 caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat cggaaaaccc
     6421 gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag cattactggc
     6481 agagcggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc atcgcccggt
     6541 acgtgatatt gatcgtgaac gcgatttact ggaaagatta attacgctcg gtaaagcgca
     6601 ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag attccgtatt
     6661 aactcagcag gctttgctcc aacaacatct caataaaatt aatccgcact cagcacgcat
     6721 cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt atgctgcccg
     6781 tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt ttaatcaggt
     6841 ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct ccggtgccat
     6901 aaatgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg agatgacgtt
     6961 aactatcgac cattgtttgt tagtctccgg cacgactgat ttatccacca tcaatacggt
     7021 ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt atccgcactg
     7081 gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac aggcaaaatc
     7141 accgcatgtt gctgcgttag gaagcgaagc tggcggcact ttgtacggtt tgcaggtact
     7201 ggagcggatt gaagcgaatc agcgacaaaa cttcacccga tttgtggtgt tggcgcgtaa
     7261 agccattaac gtgtctgatc aggttccggc gaaaacgacg ttgttaatgg cgaccgggca
     7321 acaagccggt gcgctggttg aagcgttgct ggtactgcgc aaccacaatc tgattatgac
     7381 ccgtctggaa tcacgcccga ttcacggtaa tccatgggaa gagatgtttt atctggatat
     7441 tcaggccaat cttgaatcag cggaaatgca aaaagcattg aaagagttag gggaaatcac
     7501 ccgttcaatg aaggtattgg gctgttaccc tagtgagaac gtagtgcctg ttgatccaac
     7561 ctgatgaaaa ggtgccggat gatgtgaatc atccggcact ggattattac tggcgattgt
     7621 cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg taatcgccga
     7681 accagtgctc caccttgcgg aaactgtcaa taaacgcctg cttatcgccc tgctccagca
     7741 actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga ttacgctctg
     7801 acgacataat aatgtcggca taaagctgcg gatcctgagc gaacagtcgc ccgaccatcg
     7861 ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc tgaacatttt
     7921 cttctgccag gtgcagcccg taagcaaaag tagcaaagtg gcgcagtgcc tgaataaacg
     7981 ccatattctg atcgtgctcg acggcgctaa tacgatgcaa ccgagcgccc cagacctgaa
     8041 tttgctccag aaaccattgg tatgcttccg gtttacgtcc atcacaccag accacaactt
     8101 gctttgccag gctaccgctg tccggaccga acattgggtg taaccccagt accgggccat
     8161 cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga tcaaccagaa
     8221 tacaatcttt cggtaaaggc ggtaatttgc caataacttg ctcagtaacg tggattggca
     8281 cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc cagtcatgtt
     8341 gctccagaat ccgcacctga taacccgaga gtgtcagcat cttctcgaac agacgtccca
     8401 tctgaccgcc gccgccaacg ataactaccg ggcgtaacgc aggacaaagc gttttaaatc
     8461 ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca tcctcaatca
     8521 gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac atagatgcct
     8581 ctcgctccgg aacataaata ggcagtccaa agcggctttt cacctcgccc acttcagcaa
     8641 ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca tcaatttgat
     8701 cgcgtaatgc ggtcaattca gcaaccataa taaacctctt aagccacgcg agccgtcagc
     8761 tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc ccagctaatg
     8821 caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc ggaagactga
     8881 ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt gccatctttg
     8941 atttgagcaa ccacggattc tgccaccgca ggctgacggc gataatcttt attggaatta
     9001 ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat ctctttttca
     9061 cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag gatgacatgc
     9121 ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt aatgccaaca
     9181 aaacggtgcg gctgggcggc ggcgcgcata gcgttaattg ctgttgccag actgccgtcg
     9241 gtgccgtttt taaagccaac cggcatggaa agcccggagg ccatttcacg gtgagtttgc
     9301 gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag gtattgcggg
     9361 ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag ctcaagcagc
     9421 aatttacgcg cgatctgcag cccggcttct acatcaaaag agccatccat atggggatcg
     9481 ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac gcgcattacc
     9541 agatagaggc tatcgctgac ctctgcggca agggctttaa atcgacgagc atattccaga
     9601 gcagtttccg gatcatgaat ggaacaaggg ccacatacta ccagcagacg aggatcgcgc
     9661 ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc ttgttgcagg
     9721 cttaatggaa aagcggcctt cagttgtgcc ggagtcatta aaacctgttc gtcggtaata
     9781 tgtacgttat tcagcgcgtc tttttgcatg atggcgatcc tgtttatgct cgtttgcgat
     9841 agttgatcct cagcgaggat gacgtaacga taacacataa agtaaagttt tcaatccata
     9901 tttcgtacat ttttatttac ataagcaatt tattcgcgct ttcaaccctt gattctgtat
     9961 agataaattt acaccacctt tgaaaacaat ccgctatgct ttgaaaaagg agaaagaaat
    10021 gatgaaaaag tttatcgccc ccttgttggc tttactggtt agcggatgcc agattgatcc
    10081 ttatactcac gcgccaacct tgaccagcac cgactggtat gatgttggta tggaagatgc
    10141 gatatcgggc agcgccataa aagatgacga tgcatttagc gattcacagg cggatcgcgg
    10201 tctatacctt aaaggatatg ccgaaggaca aaagaaaact tgccagaccg attttactta
    10261 tgcccgagga ctttccggta aaagctttcc tgccagctgc aacaatgttg aaaatgccag
    10321 ccaactgcat gaagtatggc aaaaaggggc tgatgaaaat gccagcgcaa tacgattaaa
    10381 ttaattaaaa ccccacaaat aaattaaaca taagatttta ctcattatgt tttatttctc
    10441 acaatgatga cgaccgcatg aaataatgat attttaatga gcattctgaa aaaatatcga
    10501 tgcatttcga gcgaagatgg tggggatccc tgaatgcgtt tttctcaccg actgttctta
    10561 ctgctgattc tcctgctgac gggagcacca atactcgctc aggagccttc tgacgttgcg
    10621 aaaaacgtgc gtatgatggt ttccggtatt gtcagttaca cccgctggcc tgcgctatca
    10681 gggccaccaa agttatgtat cttttcatct tcacgtttta gtaccgccct acaagagaat
    10741 gccgcaacat ctttgcctta tctgcctgtc atcattcaca ctcaacaaga ggcgatgatt
    10801 tcaggctgca atggttttta ttttggcaac gagtcaccga cattccagat ggaattaacc
    10861 gaacaatatc cgtcaaaggc gttattatta attgccgaac aaaataccga gtgcattatt
    10921 ggtagcgcct tttgtctgat catccacaat aatgacgtca gatttgccgt aaacctggat
    10981 gccttatcgc gtagcggtgt aaaggtcaac ccggatgtct taatgctcgc acggaagaaa
    11041 aatgatggat aacgataatt ctcttaataa gcgccccacg tttaaaagag cattacgcaa
    11101 catcagtatg accagcatat ttatcactat gatgctgatc tggttgctgc tttccgtgac
    11161 ctcggtgctg cccctgaaac agtacgcgca aaaaaacctg gcactgacag cagcaacaat
    11221 gacttacagt ctggaagcag ctgtcgtttt tgccgatggc cctgcagcaa ctgaaacact
    11281 ggcagcgctg ggccagcaag ggcaattttc aactgcagaa gtacgtgata agcagcaaaa
    11341 tattctggca tcctggcatt acacccgtaa ggatccaggc gatactttca gcaatttcat
    11401 aagccactgg ctcttccctg cccccatcat tcagccgatt cgtcacaatg gtgaaaccat
    11461 tggcgaagta cgcttaaccg ctcgcgacag ttcaatcagc cattttatct ggttttcgct
    11521 cgccgtactg accggttgta ttctgctggc atcaggaatc gcaattaccc tcacccgcca
    11581 tttgcacaat ggcctggtgg aagcactgaa aaatatcacc gatgtcgtac atgatgtgcg
    11641 ttccaaccgc aatttttccc gacgagtttc ggaagaacgt atcgctgagt ttcaccgctt
    11701 cgctctcgac ttcaacagtc tgctggatga aatggaagag tggcagcttc gtttacaggc
    11761 caaaaatgcg cagcttctac gtaccgcgct acatgaccca ttaaccgggc tggctaaccg
    11821 cgcagcgttt cgtagcggca tcaatacgtt gatgaacaat tccgctgccc gaaaaacgtc
    11881 ggcgttacta tttcttgatg gcgataattt caaatatatc aatgatacct ggggtcatgc
    11941 gacgggcgat agagtcttga ttgaaatcgc aaaacggtta gctgaatttg gcgggctgcg
    12001 acataaagca taccgcctgg gcggcgatga attcgctatg gtgctctatg gtgtacagtc
    12061 ggaatctgaa gtgcagcaga tatgctcagc actgacacaa atctttaatc tcccgtttga
    12121 tcttcataat ggccatcaga ccaccatgac attaagcatt ggttacgcaa tgaccattgc
    12181 gcacgcttct gcggaaaaat tacaagagct ggccgatcac aatatgtatc aggccaaaca
    12241 ccagcgtgcc gaaaagctgg tgagataaca aggatatatc gatatgataa agcacctggt
    12301 agcacccctg attttcacct cactaatact gactggctgc cagtcccctc agggaaagtt
    12361 tactcctgag caagtcgccg ctatgcaatc ttatggattt actgaatccg ccggcgactg
    12421 gtcgctgggc ttatcagatg ccattctgtt cgcaaaaaat gactacaaat tgctcccgga
    12481 aagccagcaa cagatccaaa ccatggcagc caaattggcc tcgacagggc taacacatgc
    12541 ccgtatggat ggacacaccg ataactatgg tgaagacagt tacaacgaag gcttatcatt
    12601 gaaacgggcg aatgtcgtgg ccgatgcatg ggctatgggt ggacaaattc cacgcagcaa
    12661 tctcaccaca cagggtttag gaaaaaaata tcccatagcc agtaacaaga ccgcccaggg
    12721 ccgcgccgag aaccgccgcg tcgcagtggt gattactacc ccttaaaaca cgagcaaaca
    12781 gataaaaaaa gggccagcca attggccagc ccttcttaac aggatgtcgc ttaagcgaaa
    12841 tcttagttaa gacgctcttt gatacgagca gccttaccag tacgctcacg caggtagtac
    12901 agtttagctt tacgaacagc accacgacgt ttgacagaaa tgctgtcaac taccggagag
    12961 tgagtctgga agacacgctc aacgccttcg ccgttggaaa ttttacgaac agtgaatgca
    13021 gagtgcagac cgcggttacg aatagcgata accacgccct cgaatgcctg cagacgtttt
    13081 ttggaacctt caacaaccca tactttcact tccacggtat cacccggacg gaaggaaggt
    13141 acgtcctgct tcatctgctc ttgttcaagt tgcttaataa tgttgctcat aatttaatct
    13201 cttatcctgg gtaaactgat atctcggggg cttacgccat cccatcatgt ttatgttgct
    13261 gttgtgcgtg ttccgttttg aactccgcca gcaaccttgc ttgctcttca gtcagagcca
    13321 ggttttccag aagttcaggt cttctaagcc aggtacggcc cagcgactgt ttcaaacgcc
    13381 agcgacgtat ctcggcatgg ttgcccgaca gtaacactgg cggaacttcc atcccttcta
    13441 acacctcagg ccgcgtatag tgcgggcaat ccagcaatcc ttcagcaaag gaatcttccg
    13501 ttgccgaggc ttcatgtccc agtactcccg gaataaaccg ggaaacggag tcaatcagcg
    13561 tcattgctgg taactcacca ccactgagaa cgtaatcgcc gattgaccat tcttcgtcaa
    13621 tttcggtttg gatcacgcgc tcatctatac cttcgtagcg accgcacacc agaatcaatt
    13681 tttgattcgt tgccagttcg ctgacgcccg cttgatcaag cttgcgtccc tgtggtgaaa
    13741 gataaatcac ctttgcgcct tcacccgccg cggcttttgc tgcatgaatg gcgtcccgca
    13801 agggttgcac catcattaac atccccggtc cgccgccgta aggacgatcg tccacggtac
    13861 ggtgccggtc atgcgtgaaa tcgcgaggac tccagctctg gatgctcagc aggccatttt
    13921 taactgcccg gccagttacc ccgtaatcgg taattgcgcg gaacatttca ggaaacaggc
    13981 taattatgcc aatccacata gcgccgtctt ttaccgttta tccggtggtt taaaaaccag
    14041 gatcccaatc tacttcgatt gaacgagtag tgagatcgac tttcttgata acctgcccat
    14101 cgaggaacgg tacgagacgt tccttgatac caaacgcatc tttcaggttt gccttaatga
    14161 cgagaacgtc attggatccg gtttccatca tatcgacgac tttaccgaga tcgtagcctt
    14221 cagtggttac tacctggcag cccatcaggt ctttccagta gtagtcaccc tcttcaagct
    14281 gaggcagctg cgatgaatcc acgacaattt cacaattcgt cagcaggttc gccgcatcac
    14341 gatcgtcaac gcctttcagc ttgatgatca tgtcctgatt gtggtgcttc cagctttcca
    14401 gctggacttg ctgccactga cccgccttct ggataaacca gggctgatag tcaaaaatgc
    14461 tttcggcgtc ttcggtggaa gaaaacactc tgagccaccc acgaataccg taagacgaac
    14521 ccatttttcc caaaacgatg ggatcaacag gtgcttgcgc ggtgagttgt ttgctcatca
    14581 tgaccaccgt gacagattaa gctgctttgt ttacttcttt gatcagcgca gcaacgcgat
    14641 cagaaatagt tgcgccctgg ccaacccagt gagcgatgcg atccagatcc aggcgagtgc
    14701 cttcttcttt ttcgctagcg attgggttga agaaaccaac gcgctcgatg aagcgaccgt
    14761 tgcgtgcatt acggctgtca gcgacaacaa cctggtagaa cggacgcttt ttagcgccgt
    14821 gacgtgctaa acgaatagtt accataacat cctcttgtgt gaataaaaca accggacccc
    14881 atcgaggaac ggagtccggt gtcatattaa aagcccgaaa attttactga tttctggaga
    14941 aaatgcaatc agcagttaat aactctgtcg cataaggtca tttagcgacc aggaaatccc
    15001 ggcggcatca tccccttcat gcttctcatc atcttcgcca ttccgccctt cttcattttc
    15061 ttcatcatgc gctgcatgtc gtcgaactgt ttcagaagac ggttaacgtc ctgcacctgc
    15121 ataccgcaac cggcagcaat acggcgttta cgcgaacctt tgatgatttc tggcttagcg
    15181 cgctctttca tcgtcatcga gttgatgatg gcttccatac gcaccagcac tttatcgtcc
    15241 atctgtgact tgacgttatc cgggatctgc cccatgcccg gcagtttgcc catcaggctg
    15301 gccatgccgc ccatattttt catctggcgc agttgctcaa ggaagtcgtt gagatcgaag
    15361 ccgtcacctt ttttcagctt gctggctaat ttctctgcct gcgcgcggtc aactttgctt
    15421 tcgatatctt cgatcagcga cagtacgtcg cccatgccga gaatacgcga cgcgatgcgg
    15481 tccggatgga acggctccag cgcctcagtt ttctcgccaa caccgaggaa cttgatcggt
    15541 ttgccagtga tgtgacgaat agagagcgcc gcaccgccgc gggcatcgcc gtccactttg
    15601 gtcaacacta cgccggtaag cggtaacgct tcattgaatg cttttgccgt attggccgca
    15661 tcctgaccgg tcatggcgtc aaccacaaac agggtttcaa ccgggttaat cgacgcatgg
    15721 acttgtttga tctcgtccat catcgcttcg tcaacgtgca gacgaccagc ggtatccacc
    15781 agcagcacgt cgtagaattt cagtttggct tctttcagcg ccgcgttaac aatatcaacc
    15841 ggcttctgac caacatcaga agggaagaaa tcaacgccca cctgctctgc cagcgtctca
    15901 agctgtttga ttgccgccgg gcgatagacg tcagcagaaa ccaccagcac tttcttcttg
    15961 tgcttctcgc gcaggaactt accgagttta ccaacgctgg ttgttttacc ggcaccttgc
    16021 aggcctgcca tcagcacgac cgcaggcggt tgcgcagcca gattcagggt ctggttctct
    16081 tcgcccatcg ccgcaaccag ttcgttacgg acgattttga cgaactcctg ccccggcgtc
    16141 aggctcttat taacttcatg accaaccgct ttctctttta cgcgattgat aaactcacgc
    16201 actaccggca gagcgacgtc cgcctccagc agcgccatgc gcacttcgcg cagcgtatct
    16261 tttacgttgt cttcagtgag gcgtccacgg ccactgatat tgcgcagcgt gcgcgacaaa
    16321 cgatcggtta aattatcaaa cattgtctct cgcctggggt ggaaacggtt ggccgcaatc
    16381 gcgacacatc atcagtattt tgccgcagta taacatgaag gcgtctttgt tgttatgcaa
    16441 cggttggagc agcgttcacc tgacgctata ctgcttctct ttcttattgc tcaaactgtc
    16501 gacatcacta tgcccgtttt tgctctgctc gcgcttgtcg cctactccgt cagtcttgcg
    16561 ctgattgttc ccggtctgct gcaaaaaaac ggcggctggc ggcgcatggc tattatttct
    16621 gcggtcattg cgctggtctg ccacgcaatc gctctggaag cccgcatcct gcccgacggt
    16681 gatagcggac aaaacctcag cctgctgaac gttggttcat tggtcagttt gatgatctgt
    16741 acggtaatga ccattgtggc ttctcgcaat cgtggctggt tgctgctacc cattgtctat
    16801 gcctttgcgc ttatcaacct ggcgctggca accttcatgc ccaatgaata catcacccat
    16861 ctggaagcta cgcctgggat gctggtgcac attggcttat cgctcttttc ctatgccacg
    16921 ctaattatcg ccgccctgta cgcgctgcaa ctggcgtgga ttgattacca actgaagaac
    16981 aagaagctgg cgtttaacca ggaaatgccg ccattgatga gtatcgagcg taaaatgttc
    17041 cacatcacgc agattggcgt ggtgctgcta acgctcacgc tttgcactgg cctgttctac
    17101 atgcacaact tatttagcat ggaaaatatc gacaaggctg tgctctctat cgtggcgtgg
    17161 tttgtctata ttgtgctgct atggggacat tatcatgaag gatggcgtgg acgtcgcgtc
    17221 gtctggttta acgttgcggg tgcagtcatt ctgacactgg cctacttcgg cagccgaatt
    17281 gtccagcagt taatcagcta aacccagaaa aggagtttcc ccctggaaca catttctact
    17341 actacgttga tcattattct gatcatcatg gtggtcattt cagcctattt ttccgggtcc
    17401 gaaaccggaa tgatgaccct caaccgctat cgtctgcgac atatggcgaa acagggtaat
    17461 cgctcggcca aacgcgtcga aaaattgctg cgtaagccag accgcctgat aagcctggtg
    17521 ttaatcggca ataacctggt caatattctt gcctccgcgc tcggcactat tgttgggatg
    17581 cgtttgtacg gcgatgcggg cgtggcaatt gcgactggtg tgctgacttt tgtcgtactg
    17641 gtttttgctg aggtattgcc gaaaaccatt gccgcgctgt acccggaaaa agtcgcttat
    17701 ccgagtagtt ttctgctggc tcccctgcaa attttgatga tgccgctggt ctggttgctg
    17761 aatgctatca cccgtatgct gatgcgcatg atgggtatca aaaccgatat cgtggttagc
    17821 ggctctttga gcaaagaaga gttgcgcact atcgtgcacg aatcgcgctc acaaatttcc
    17881 cgtcgcaatc aggatatgct gctgtcggtg ctcgatctgg aaaaaatgac cgttgatgac
    17941 atcatggtgc cgcgcagtga aattatcggt attgatatca acgatgactg gaaatctatt
    18001 ctgcgccaac tctcccactc acctcacggg cgcatcgtgc tctaccgtga ttcgctggac
    18061 gacgccatca gtatgctgcg agtacgtgaa gcttggcggt tgatgtcgga gaaaaaagag
    18121 ttcaccaaag aaaccatgct gcgcgccgcg gacgagatct attttgtgcc ggaaggtacg
    18181 ccgctcagca cgcagttggt gaagtttcag cgcaacaaaa agaaagtcgg cctggtcgtc
    18241 aacgagtatg gagacattca ggggctggtg acggttgaag atattctgga agagattgtc
    18301 ggcgatttca ctacgtcgat gtcgccaaca cttgccgaag aagtcacgcc gcaaaacgac
    18361 ggttcggtga ttattgatgg cactgccaac gtgcgggaaa tcaacaaagc ctttaactgg
    18421 catctaccgg aagatgatgc ccgcacggtt aatggcgtca ttcttgaggc actggaagag
    18481 atccctgtcg taggcacccg cgtgcgtatt ggcgagtacg atatcgatat tctcgacgta
    18541 caggacaata tgattaagca ggtaaaagtt tttcctgtga aaccgctgcg cgagagtgtg
    18601 gcggagtaac gaaaacggcc cggcattcgc atgcagggcc gtgaattatt acgaaaacag
    18661 aaattacgct tttgctttcg ctacagttac catcgccgca cgaatcgtac gaccattcag
    18721 cgtataaccc ttctgcataa tgcccagtac gttacctggc gcaacgtcat cagattccac
    18781 cattgcgata gcctgatgca cattcgggtc cagcggcacg ttagtttcgg cgatcacttc
    18841 aacaccaaac ttacgcacaa catccagcat cgacttcagc gtcagctcaa tgccttcaac
    18901 catcgcagac atatccgggt tagctttatc agccacttcc agcgcacgat ccaggctatc
    18961 aatcaccggc agcaattcgt tgatgaattt ctccagcgcg aatttgtggg ctttttcaat
    19021 atccagttca gtacgacgac gcaggttttc catttcggct tttacacgca aaatgccgtc
    19081 acgttcacgg gtctgggctt cagccagctg agcttcgaga ttcgcaattt tttcatcgcg
    19141 cggatccacc tgctcagcag aagcttctgg ctcaactgcc tcaatctctt cgtgctgatc
    19201 catgataatt tcttccgggg cttgcccctc aggcgttttc tgttctttac tactcatgaa
    19261 tttctccgcg tttttttcgc attcatctcg ctaacttcgc ttattatggg gatcagtttc
    19321 agggtttcaa gggaagcact cacattgtca tcaatcttcg caacaaggac ctcggaaaaa
    19381 tgaataatca tttcaagtgt attggcattg tgggacaccc acggcaccct actgcactga
    19441 caacacatga aatgctctac cgctggctgt gcacaaaagg ttacgaggtc atcgttgagc
    19501 aacaaatcgc tcacgaactg caactgaaga atgtgaaaac tggcacgctc gcggagattg
    19561 ggcaactggc tgatctcgcg gtagtcgttg gtggcgacgg taatatgctg ggcgcgacgc
    19621 gcacgctcgc ccgttacgat attaaagtta ttggaatcaa ccgtggcaac ctgggtttcc
    19681 tgactgacct tgaccccgat aacgcccagc aacaattagc cgatgtgctg gaaggtcact
    19741 acatcagcga gaaacgtttt ttgctggaag cgcaagtctg tcagcaagat tgccaaaagc
    19801 gcatcagcac cgcgataaat gaagtggtac ttcatccagg caaagtggcg catatgatag
    19861 agttcgaagt gtatatcgac gagatctttg cgttttctca gcgatctgat gggctaatta
    19921 tttcgacgcc aacaggctcc accgcctatt ccctctctgc gggcggtcct attctgaccc
    19981 cctctctgga tgcgattacc ctggtgccca tgttcccgca tacgttgtca gcacgaccac
    20041 tggtcataaa cagcagcagc acgatccgtc tgcgtttttc gcatcgccgt aacgacctgg
    20101 aaatcagttg cgacagccag atagcactac cgattcagga aggtgaagat gtcctgattc
    20161 gtcgctgtga ttaccatctg aatctgattc atccgaaaga ttacagttat ttcaacacat
    20221 taagcaccaa gctcggctgg tcaaaaaaat tattctaatt ttacgccagc ctctttactg
    20281 tatataaaac cagtttatac tgtacacaat aacagtaatg gtttttcata caggaaaacg
    20341 actatgttgg cacaactgac catcagcaac tttgctatcg ttcgtgagct tgagattgat
    20401 tttcatagcg gcatgaccgt aataactggc gaaaccggtg cgggtaaatc tattgcaata
    20461 gatgccctcg gtctttgtct cggtggtcgc gctgaagccg acatggtgcg taccggcgct
    20521 gctcgcgctg acctgtgcgc ccgtttttct ctgaaagata cgccagcggc cctgcgctgg
    20581 ctggaagaaa accagcttga agacgggcat gaatgtttgc ttcgtcgcgt gatcagcagc
    20641 gatggtcgct cccgtggttt catcaacggt acagctgttc ctctgtcaca actgcgcgaa
    20701 ctgggtcagt tgctgattca gatccatggt cagcacgctc atcagttact caccaaaccc
    20761 gagcaccaaa aattcctgct tgatggctat gccaatgaaa cctctctact ccaggaaatg
    20821 accgcacgtt atcagttgtg gcatcaaagc tgccgtgacc tcgcgcatca tcaacagtta
    20881 agtcaggaac gcgctgcccg tgcggaactg ctgcaatacc aattaaaaga acttaacgaa
    20941 tttaatccgc agcccggaga gtttgagcaa atcgacgaag agtacaaacg tctggcgaac
    21001 agcggtcaat tgctgaccac cagccagaat gcattggcat taatggccga cggtgaagac
    21061 gcaaacctgc aaagtcagct ttacacggct aaacaactgg tgagcgaatt gattggcatg
    21121 gacagcaaac tgtccggcgt acttgatatg ctggaagaag ctaccatcca gattgttgaa
    21181 gccagcgacg aactgcgcca ctactgcgat cgtctggatc tcgatcccaa ccgactgttt
    21241 gaacttgaac agcgcatctc aaaacagatt tcgctggcac gtaaacatca cgtcagccct
    21301 gagacattgc cacagtatta ccagtcgcta ctggaagaac agcagcaact ggacgatcag
    21361 gccgactcac aagaaacgct cgcactggcg gtaacgaaac atcatcagca ggcactggaa
    21421 acggcgcgcg cattacacca gcaacgccag cattatgcag aagaacttgc acagctgatc
    21481 accgacagta tgcatgcgct ctcaatgccg catgggcagt ttacgattga tgtcaaattt
    21541 gacgagcatc acctgggcgc tgacggcgcc gatcgtattg agtttcgggt aaccaccaac
    21601 ccaggtcagc caatgcagcc tattgccaaa gtcgcatccg gtggtgaatt gtcccgcatc
    21661 gcattggcaa ttcaggtcat cacggcgcgt aaaatggaaa ccccggcact gatttttgat
    21721 gaagtggatg tagggattag cggtccaaca gcggcagttg tcggcaaact gctgcgtcaa
    21781 ctcggcgaat caactcaggt gatgtgtgtt acccacctgc cacaagtcgc gggatgtggt
    21841 catcaacact attttgtcag caaagaaacc gatggtgcga tgacagaaac gcatatgcaa
    21901 tccctgaata aaaaaacgcg gttacaagag ctggcgcgcc tgcttggtgg cagtgaagtc
    21961 acacgtaaca cactggcgaa tgcgaaagaa ctgcttgcgg cgtaaacttt tttcctgctt
    22021 cacggtcaga gtaaacagca aaacgccgta agaccggaaa gcaaaaggtt ttaaagtgat
    22081 gaaaggtcta ttatcatcgg catattacag atgagccacg tactgctcgg gcccgaaaag
    22141 gaatcaaatc actatgcgct gtaaaacgct gactgctgca gcagcagtac tattgatgtt
    22201 gaccgcaggc tgttccactc tggagcgagt ggtttaccgt cctgacatca accaggggaa
    22261 ctatctgacc gctaacgacg tatccaaaat acgtgttggc atgacgcaac aacaagttgc
    22321 gtacgcattg ggtacaccgc tgatgtccga tccatttggt acgaatacct ggttctatgt
    22381 cttccgccag caaccaggtc atgaaggtgt aacgcagcaa acgctgacgc taacctttaa
    22441 cagtagcggt gtgttgacca atattgataa caaacctgcg ctgagtggta actaataata
    22501 aagttgctct caaagacgtt aaaaaaggtg ctcaatgagc accttttttc tgtccgttat
    22561 ttattcgctg atttttctgc tcgttgcctg cgaagctctt tcggatcggc aatgagaggg
    22621 cgataaatct ccacccgatc gccatcatgc acactatcgc ttaattttgc cggacggctg
    22681 taaatgccga ctttgttttc agttaaatcg atatcggtac gcaattccag caagccactg
    22741 gcgcgaatag cttcttcaac cgtcgcgccc tcctgcagcg tcacgcgctg caggtactgc
    22801 ttcttaggta gcgcataagc cacctcaacg gcaattttac ctggcactgt aaacctcttt
    22861 cgcacgaacc gtaaaagcct ggaccatatt agacgccagc tctttaaaca cgcgaccaaa
    22921 agcgagttca atcaacttat tggtaaactc aaagtcgaga tgaaactcga tacgacacgc
    22981 ctcctggctc agcggcgtaa acttccatcc accaatcaat ttcttgaacg gcccatccac
    23041 cagattcata agaatacttt ggttactggt caactggttg cgggtagtaa acgttttgct
    23101 gatcccagcc ttagagacat ctaccgccgc agtcatctgc ccaggagtgg actccagaat
    23161 ccgacttccg gtacaacccg gcaaaaactg aggataagac tgaacgtcat tcactaactg
    23221 atacatttgc tccgcgctgt agggtaccag tgcggtccgg ctaatctgag gcataacaat
    23281 ttccatcaac aaaaatccaa caaataatat catttatcct gcagtaaaaa aaacgctatc
    23341 ccggcgctgg gtaacatccg gttcatgcta agatagagcc ttgtcccccg caggattgat
    23401 atggggtatt ttcgatttca gattaccgat gattcacgac gcttatgacg aagaaaaaag
    23461 cacataaacc tggttcagcg accatcgcgc ttaacaagcg cgcccgtcac gaatacttta
    23521 ttgaagaaga gttcgaagcg ggacttgccc tgcaaggctg ggaagttaaa tccctgcgcg
    23581 caggaaaagc caatattagc gacagctatg tccttctgcg cgacggagaa gcgttcctgt
    23641 ttggcgcaaa catcacgcca atggctgtgg cttctacgca tgtggtgtgc gatcctaccc
    23701 gtacccgcaa gttacttctc aaccagcgcg aacttgattc gttgtacggt cgcgtcaatc
    23761 gagaaggcta taccgtagtg gcgctctccc tgtactggaa aaatgcctgg tgcaaagtga
    23821 aaatcggcgt cgccaaaggt aagaaacagc acgataaacg ttcagatatc aaagaacgcg
    23881 aatggcaggt ggataaagcg cgtatcatga aaaacgccca ccgttaaacc tgcactccaa
    23941 ttcttgacct gttcctcacc gcgcctccct ctccggcggc gcgaatgaac atcttattgg
    24001 ctatcacatc cgacaaaaat gttgccatcc cattgcttaa tcgaataaaa atcaggctac
    24061 atgggtgcta aatctttaac gataacgcca ttgaggctgg tcatagcgct cataaatctg
    24121 gtatacttgc cgttacacat tggggctgat tctggattcg acgggatttg cgaaacccaa
    24181 ggtgcatgcc gaggggcggt tggcctcgta aaaagccgca aaaaatagtc gcaaacgacg
    24241 aaaactacgc tttagcagct taataacctg cttagagccc tctctcccta gcctccgctc
    24301 ttaggacggg gatcaagaga ggtcaaaccc aaaagagatc gcgtggaagc cctgcctggg
    24361 gttgaagcgt taaaacttaa tcaggctagt ttgttagtgg cgtgtccgtc cgcagctggc
    24421 aagcgaatgt aaagactgac taagcatgta gtaccgagga cgtaggaatt tcggacgcgg
    24481 gttcaactcc cgccagctcc accactttaa gaaggactac aaccggacag tagcaataaa
    24541 tacagccact tacggacaca agccagacag tagtcggact gaaaaaagac aaaaatatac
    24601 acgtgaaatg cacgtgcact tctaaaaaac cccagttatc accgactggg gtttttcaat
    24661 ttgtaactaa gggtaacaac ccccccgttt ttttcatctc actcaccttg atactgttta
    24721 tttgtacagt taaaaataat actgtataca aacacagtat agagggactt ttatgcgtat
    24781 tgagatctgc atagccaaag aaaaaat
//
LOCUS       DAZKIP010000063        24611 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZKIP010000063 DAZKIP010000000
VERSION     DAZKIP010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.063"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..134
                     /locus_tag="ACHOUK_002782"
     CDS             <1..134
                     /locus_tag="ACHOUK_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector EspY1"
                     /protein_id="HFP9898195.1"
                     /translation="DGATHLYDEVIIINKITPKKIDTEEVATKQSTAEQITDNAIIE"
     gene            complement(161..339)
                     /locus_tag="ACHOUK_002783"
                     /pseudo
     CDS             complement(161..339)
                     /locus_tag="ACHOUK_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
     gene            complement(552..1211)
                     /gene="rluA"
                     /locus_tag="ACHOUK_002784"
     CDS             complement(552..1211)
                     /gene="rluA"
                     /locus_tag="ACHOUK_002784"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFP9898196.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFRERVPKKQYVTRVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(1223..4129)
                     /gene="rapA"
                     /locus_tag="ACHOUK_002785"
     CDS             complement(1223..4129)
                     /gene="rapA"
                     /locus_tag="ACHOUK_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFP9898197.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            complement(4293..6644)
                     /gene="polB"
                     /locus_tag="ACHOUK_002786"
     CDS             complement(4293..6644)
                     /gene="polB"
                     /locus_tag="ACHOUK_002786"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFP9898198.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILRGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASALDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLVSSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEATKIG
                     RALVQHVNVWWAETLQKQQLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYFRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            complement(6719..7414)
                     /gene="araD"
                     /locus_tag="ACHOUK_002787"
     CDS             complement(6719..7414)
                     /gene="araD"
                     /locus_tag="ACHOUK_002787"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFP9898199.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSIMTADDMVVVSIETGEVVEGAKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            complement(7614..9116)
                     /gene="araA"
                     /locus_tag="ACHOUK_002788"
     CDS             complement(7614..9116)
                     /gene="araA"
                     /locus_tag="ACHOUK_002788"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFP9898200.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVKALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAAEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            complement(9127..10827)
                     /gene="araB"
                     /locus_tag="ACHOUK_002789"
     CDS             complement(9127..10827)
                     /gene="araB"
                     /locus_tag="ACHOUK_002789"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFP9898201.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCTTGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRSEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKEQIDASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            11166..12044
                     /gene="araC"
                     /locus_tag="ACHOUK_002790"
     CDS             11166..12044
                     /gene="araC"
                     /locus_tag="ACHOUK_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFP9898202.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            12130..12894
                     /gene="yabI"
                     /locus_tag="ACHOUK_002791"
     CDS             12130..12894
                     /gene="yabI"
                     /locus_tag="ACHOUK_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFP9898203.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLVRHPLMPVYIDILRKVVGV"
     gene            complement(12981..13679)
                     /gene="thiQ"
                     /locus_tag="ACHOUK_002792"
     CDS             complement(12981..13679)
                     /gene="thiQ"
                     /locus_tag="ACHOUK_002792"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFP9898204.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGALTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAVQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGMTNELL
                     SGKASASALLGITG"
     gene            complement(13663..15273)
                     /gene="thiP"
                     /locus_tag="ACHOUK_002793"
     CDS             complement(13663..15273)
                     /gene="thiP"
                     /locus_tag="ACHOUK_002793"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFP9898205.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGDWS
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVTALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTMLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFSVIEKIPGRNVKTD"
     gene            complement(15249..16232)
                     /gene="thiB"
                     /locus_tag="ACHOUK_002794"
     CDS             complement(15249..16232)
                     /gene="thiB"
                     /locus_tag="ACHOUK_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFP9898206.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPVVKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDIFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEKLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     regulatory      complement(16263..16362)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            16604..17173
                     /gene="espY2"
                     /locus_tag="ACHOUK_002795"
     CDS             16604..17173
                     /gene="espY2"
                     /locus_tag="ACHOUK_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspY2"
                     /protein_id="HFP9898207.1"
                     /translation="MKVRNPEQISIPASNTTKDPGLTNSQIIRMANLVKKTENMNIFE
                     ELWETLRNLFQSDKHSQTAARQILKDAFYFQNSDDYSKYFTGAVDGKARDKLTHWLIK
                     FNELKEYAKDPENMAAKASLSPEGTLCVSFFIGDEAIFTLELQLKKSTRTGGIDLSNA
                     YFNGVVICGIDCLEVDLSNAETNNSRWYD"
     gene            complement(17402..19060)
                     /gene="sgrR"
                     /locus_tag="ACHOUK_002796"
     CDS             complement(17402..19060)
                     /gene="sgrR"
                     /locus_tag="ACHOUK_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFP9898208.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     ITSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQVDAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDPE"
     gene            19149..19280
                     /gene="sgrT"
                     /locus_tag="ACHOUK_002797"
     CDS             19149..19280
                     /gene="sgrT"
                     /locus_tag="ACHOUK_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFP9898209.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWEGSHSDY"
     gene            complement(19388..19993)
                     /gene="leuD"
                     /locus_tag="ACHOUK_002798"
     CDS             complement(19388..19993)
                     /gene="leuD"
                     /locus_tag="ACHOUK_002798"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFP9898210.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFSQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMR"
     gene            complement(20004..21404)
                     /gene="leuC"
                     /locus_tag="ACHOUK_002799"
     CDS             complement(20004..21404)
                     /gene="leuC"
                     /locus_tag="ACHOUK_002799"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFP9898211.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGILL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            complement(21407..22498)
                     /gene="leuB"
                     /locus_tag="ACHOUK_002800"
     CDS             complement(21407..22498)
                     /gene="leuB"
                     /locus_tag="ACHOUK_002800"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFP9898212.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     IELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            complement(22498..24069)
                     /gene="leuA"
                     /locus_tag="ACHOUK_002801"
     CDS             complement(22498..24069)
                     /gene="leuA"
                     /locus_tag="ACHOUK_002801"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFP9898213.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRATEVEKE
                     LQRKAQHNENNKETV"
     gene            complement(24162..24251)
                     /gene="leuL"
                     /locus_tag="ACHOUK_002802"
     CDS             complement(24162..24251)
                     /gene="leuL"
                     /locus_tag="ACHOUK_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFP9898214.1"
                     /translation="MTHIVRFIGLLLLLNASSLRGRRVSGIQH"
BASE COUNT         5583 a         6610 c         6625 g         5793 t
ORIGIN      
        1 tggacggtgc tacccaccta tatgatgagg taattattat taataaaatc acccccaaaa
       61 aaattgatac tgaagaagtt gctactaaac aaagtactgc tgaacaaatt actgacaacg
      121 caattattga atgagccaga ataagctaag gttgaagggg ctggaacgcc ccttcaacct
      181 tagcagtagc gtgggatgat ttcacaatta gaaagacctg catgatgagc tagagaagag
      241 gctagtgacg caaggcgtcg tgtacgacgc ggatcaccga gatgggcatc gccaaccaga
      301 ctgctaatta gcccatgaat aacaatcaga aaggaccata acagacccga taaaatgaaa
      361 tataagagac ggtcaacggg tgaagaaaaa gttcaaaaat tcgctgtgga gcaggaaggg
      421 aattaccgaa tggaaagcgt agccacacgc aacaactgaa agcagtttgg cagaaacaaa
      481 aaatccccgg actcggggat ttatgtacaa gaggcagccc ttaggatgag ggtataaacg
      541 tacaggaaag gttaaaaatc cgctggcgct ttaaacgtca tactattgcc atacgccgga
      601 tgggtaatcg tcaacatctc tgcatgtagc aacaaacgcg gtgccatcgc tctcgcttct
      661 ggtgatgcat aaaaacgatc gccgagaatc ggatgcccca gcgccagcat atgcacacgc
      721 aattgatgcg aacgcccggt aatcggtttt aacaccactc ttgccgtgtt atccgccgca
      781 tactccacca cttcatattc cgtctgcgca ggtttacccg tttcgtaaca gactttctgt
      841 ttcgggcggt ttggccagtc gcaaatcagc ggcagatcca ccagaccttc tgcgggggat
      901 ggatgccccc agacgcgggt cacatactgc tttttcggca cgcgctcgcg gaactggcgt
      961 tttaactccc gctccgcggc tttggtcagc gccactacaa tcacgccgct ggtagccata
     1021 tccagacgat gcaccgattc tgcctgcgga taatctcgct gaatgcgcgt catcacgctg
     1081 tctttgtgct cttccagacg acccggcaca gacaacaaac cgctcggctt gttgaccacc
     1141 ataatatggt catcctgata caggataacc aaccagggtt cctgcggtgg attgtagttt
     1201 tccatcccca ttttcggctc cgttactgat gcgttacaac gatcaaacgc agggcatcca
     1261 gacgccaacc tgcctgatcc aggctttcca ttacctgctg acggttgctc tcaatggcgg
     1321 tcagttcgtc gtcacgaatg ttcgggttca ctgcacgcag agcttccaga cgagacagct
     1381 cggcagacag tttttcgtcg gcttcgttac gtgctgcatc aatcaatgca cgggcagatt
     1441 tctcgatctg cgcttcaccc agttgaagga tagcgtgaac atcctgctgc acggcgttaa
     1501 ccagtttgct gccggtgtga cggttaaccg cgttaagctg gcggttaaag gtttcaaact
     1561 ctacctgcgc cgccaggttg ttgccgtttt tatccagcag catacgtacc ggcgtcggtg
     1621 gcaggaagcg gttgagctgc aactgcttcg gagcctgggc ttcaaccaca taaatcagtt
     1681 ccaccaacag cgtacctacc ggcaacgctt tgttttttaa cagtgaaatc gtgctgctac
     1741 cggtatcgcc agaaagaatc agatccagac cgttgcggat cagcggatgt tcccaggtaa
     1801 taaactgcgc atcttcacgc gccagcgcca cttcacgatc aaaggtgatg gtgatgccat
     1861 cttccgacag gccagggaag tccggcacca gcatatgatc ggacggcgtc agcacgatca
     1921 tgttgtcgcc gcgatcgtcc tgattgatac cgataatatc gaacaggttc atggcgaagg
     1981 cgatcaggtt ggtatcgtca tcctgctctt caatgctttc tgccagtgcc tgggcttttt
     2041 cgccaccgtt ggagtggatt tccagcaggc ggtcacgacc ctgttccagc tgtgctttca
     2101 gcgcttcatg ttgctcgcgg cagtttttga tcagatcgtc aaagccttcg gtttgatccg
     2161 gtgaagccag atagttaatc agatcgttgt atacgctatc gtaaatagtg cgtccggtcg
     2221 ggcaggtgtg ctcaaatgca tccagacctt cgtgatacca gcgcaccagc accgactgag
     2281 cggttttctc cagataaggc acatggatct gaatatcgtg cgcttggccg atacgatcca
     2341 gacgaccaat acgctgctcc agtagatccg ggttgaatgg caggtcaaac atcaccatgt
     2401 ggctggcgaa ctggaagtta cgtccttcag aaccgatttc tgagcacagc agtacctgtg
     2461 cgccggtgtc ttcttcggca aaccaggcgg cagcgcggtc acgttcgata atcgacatac
     2521 cttcgtggaa caccgcagcg cgaatacctt cacgttcgcg cagtacttgc tccagttgca
     2581 gcgcagtggc agctttagcg cagatcacca gcactttctg agagcgatgg ctggtcaggt
     2641 agcccatcag ccactcaacg cgcggatcga agttccacca ggtggcgtta tcaccttcaa
     2701 attcctgata aatacgctcc gggtagagca tatcgcgggc gcggtcttcc gcacttttac
     2761 gtgcgcccat aatgccggag actttaatag ccgtctgata ctgcgtcggt agcggcagct
     2821 taatggtgtg cagctcgcgt ttcgggaagc ctttcacacc gttacgggtg ttacggaaca
     2881 gcacgcggct ggtgccgtgg cgatccatca gcatcgaaac cagctcctga cgggcgctct
     2941 gggcatcttc gctgtcgctg tttgctgctt gcaacagcgg ctcgatatcc tgctcgccga
     3001 tcatctcgcc aagcatgttc agttcgtcat tgctcagttt gttacctgcc agcagcatgg
     3061 caacggcgtc cgctactgga cgataatttt tctgctcttc aacgaattgc gcgaaatcgt
     3121 ggaaacggtt cgggtccagc agacgcagac gggcgaagtg gctttccatt cccagctgtt
     3181 ccggggttgc ggtcagcagc agaacgcccg gcacgtgctc tgccagttgt tcaatagcct
     3241 gatattcgcg gctcggcgcg tcttcgctcc acaccaggtg atgcgcttca tcgaccacca
     3301 gcaggtccca ctcggcttca cagagatgtt ccagacgctg tttgctacgg cgggcaaaat
     3361 ccagcgagca aatcaccagc tgttcggtgt cgaacgggtt gtaagcatcg tgctgagctt
     3421 cggcataacg ctcatcatca aacagcgcaa agcgcaggtt gaaacggcgc agcatttcta
     3481 ccagccactg atgctgtaag gtttccggga cgataatcag cacacgttca gcagcgccag
     3541 agagcagttg ctgatgaagg atcatcccgg cttcaatggt tttccctaaa cccacttcgt
     3601 cagccagcag gacgcgcggc gcgtggcggc ggccaacatc atgagcgatg ttgagctggt
     3661 gcgggatcag gctggtacgc tgaccgcgca ggccgctgta cggcatacgg aactgttcgc
     3721 tggaatactt acgcgcgcga taacgcagcg caaagcggtc catacggtca atctgcccgg
     3781 caaacaggcg gtcctgcggt ttgctgaaca ccagtttgct atcaaggaaa acttcacgca
     3841 gggctacgcc ggactcttca gtatccaggc gagtaccgat ataggtcagc aagccatttt
     3901 cttcttttac ttcttcgact tgcatctgcc agccgtcatg gctggtaatg gtatcaccag
     3961 ggttgaacat cacgcgggtc acgggggaat cactgcgtgc gtacagacgg ttttcaccag
     4021 tagatgggaa aagtaaagtg acagttcgcg catccaccgc gacaacggtt ccaagtccca
     4081 attcgctttc tgtatcgctg atccagcgtt gaccaagtgt aaaaggcata tgtgttcggc
     4141 tctatatctt taattgcagg caataaccac ccgctaccgt gcttatgagg tagtggtgtt
     4201 attcaggtcc aggaatggaa agggcgctat ggtactggat ggcaaagcat tcgtcacgca
     4261 tcaaaatggt atctggcgaa ctcttttttt gctcaaaata gcccaagttg cccggtcata
     4321 agtgtagcaa aattatcctc aataaaaggg agtattccct ccgccacggg ttgtagctgg
     4381 cgggtcagat agtgttcgta atccagtggt gaacgttggt agtccagcgg ctccgggccg
     4441 ttggtggtcc atacgtactt aatggtgccg cggttctgat attgcaaggg gcgaccgcgc
     4501 ttttggtttt cttcatcggc aagacgagcg gcgcgtacat gaggcggaac attacgctga
     4561 tattcgctca gcggacggcg aaggcgttta cggtaaacca gccgcgcatc cagttcaccc
     4621 gccatcagtt tgtcgatggt ttcgcggaag tactcctgat atggctcgtt gcggaagata
     4681 cgcaggtaaa gctcctgctg aaactgctgg gccagcggcg tccagtccgt acgcacggtt
     4741 tccagccctt taaacaccat ccgctgtttg tcgccctcct gaatcagccc ggcataacgc
     4801 tttttactgc cggtatcggc tccgcgaatg gttggcatca gaaaacggca gaaatgggtt
     4861 tcatactcca gttccagtgc gctggtcagc tgttgttttt gcagcgtttc tgcccaccag
     4921 acgttaacgt gctgcaccag tgcacgaccg attttcgtcg cttcttcttc cgaatgtgcg
     4981 cctttcagcc agacaaacgt tgagtcggta tcgccgtaga tcacgtcgta gccctgcgct
     5041 tcaatcaacg ctttggtttg ccgcatgatc tgatgaccac gcatggtgat cgacgatacc
     5101 agccgcggat cgaagaagcg gcaggcggtg gtgccgagca cgccataaaa ggcattcatg
     5161 atgattttca gcgcctgcga cagcggtttg ttaccctggc gtttggcttc atcgcgtccg
     5221 tgccagatgt tagtcacaat ctccggcagg caatgttttt ctcgcgagaa ccaggcatcg
     5281 agaaagcctt cggtgctgtg ctctggatca ggctgcgcca tgccttccac cagcccgacg
     5341 ggatcaatca gaaaggtgcg gatgatcgac gggtacaggc ttttgtagtc cagcaccagc
     5401 accgaatcat aaagcccagg tcgcgaatcc atcacgtagc cgccagggct ggcgtgcggc
     5461 ggcacttcgc cgagattagg cgcgacataa ccagcgcgat gcatacgggg aaaatagaga
     5521 tgaccaaacg ctgccaccga cccgccgtgt cgatccaccg gcaggccgtt caccgttgct
     5581 cgttcgagca aaaacggcat gatttcagtt ttatggaaga tctgcgtcac cagctcgcaa
     5641 tctttcaggt tataagttgc cagcgcaggt ttatcttcgg cgaaacggcg gtcaatttcg
     5701 tccattcgat cccacgggtt atcgatagat tttccttcgc ctaatagctc ctgagcgaca
     5761 gtttccagcg agaatgaaga gaaattccag aacgcggatt tcagcgcctc gataccgtcg
     5821 ataattagcc gacctttagc ctgggcaaaa aagacgccgt ttttaaagcc gtgctcgcgc
     5881 cactccagct cactgttatc gcgccccagc cgcagcggaa tacgataacg ctcggcatgt
     5941 ttttgcagca ttcgcagatc aaactgcacc acgttccaac cgatgatcac atcaggatcg
     6001 tagtttgcaa accaggcgtt gagtttttcc agtagctgcg ggcggctggc gacgtactcc
     6061 agttcaaaat cgagcgcgga ggcgtcgcca ttctctggtc ccagcatata aacgatgcgc
     6121 tgcccgcagc cctccaggcc gatgcagtac agctctccgt ggcgggtggt ttcaatatca
     6181 atagaaaccc acttgagcgg cggacgatag tcgggatgcg gtttcagacg ggcattaacg
     6241 atagcgccat tgtgcatatc accctcgacc cacaccggtg aggtgataaa ccgctccatc
     6301 agatagcgtt ctggcgggcg cacatcggcc tcgtagacgg taacgccacc ttcacgcagg
     6361 cgcttttcgt aattcatcaa ctggcgatgg gcgcgacagt aaaggccata caccggctgg
     6421 cggtgaaaat cctttaacgc cagcggcgtc aggcgaaagc cttgttcacc ccgcaaaata
     6481 tgctgagcgc ggggaacctg atcggcggga ataaacgcca cggactcttg cggtgcaagc
     6541 gtaacctgca acggcccgtt gtccgtcgcc agccagaagg agacctctgt tccttgcggg
     6601 gtatcccgcc agtgtcgggt taagataaaa cctgcctgcg ccacgctgaa aatccatcaa
     6661 aaaaccaggc ttgattatag cctggtttca tttgattggc tgtggtttta tacagtcatt
     6721 actgcccgta atatgccttc gcgccatgct tacgcagata gtgtttatcc agcagcgttt
     6781 gctgcatatc cggtaactgc ggcgctaact gacggcagaa tatccccata taagcgacct
     6841 cttccagcac gatggcgtta tgcaccgcat cttcggcatt tttgccccat gcaaatgggc
     6901 cgtgagaatg gaccagaacg ccgggcattt gcgctgcatc gataccctgt ttttcgaagg
     6961 tttctacgat gacgttaccg gtttcccact catattcgcc gttgatttct gcgtcggtca
     7021 ttttgcgggt gcagggaatg gtgccgtaga aatagtcggc gtgggtggtg ccggttgctg
     7081 gaatcgactg acccgcctgc gcccagatgg tggcgtggcg cgagtgcgtg tgcacaatgc
     7141 cgccaatgga cgggaatgcc tgatagagta gtcggtgagt tggcgtatcg gaggagggct
     7201 ttttcgcacc ttcaaccact tcaccggttt cgatgctgac cacgaccata tcgtcagcgg
     7261 tcatgatgct gtaatcgacg ccggaaggtt tgatcacaaa gacgccgcgc tcgcgatcaa
     7321 cggcgctgac gttgccccat gtgagcgtga ccaggttgtg ttttggcagc gccaggttgg
     7381 cttctaatac ctgacgtttg agatcttcta acatgttgac tccttcgtgc cggatgcgcg
     7441 ttgcttatcc ggcctacaaa atcgcagcgt gtaggccaga taagacgcgt cagcgtcgca
     7501 tcaggcgctg aatgccggat gcgcgttgct tatccggcct acaaaattgc agcgtgtagg
     7561 cctgataaga cgcgccagcg tcgcatcagg cgttacatac cggatgcggc tacttagcgg
     7621 cgaaacccgt aatacacttc gttccagcgc agcgcgtctt taaacgctgg caggcgggtg
     7681 tcgttatcaa tcaccgtgat ttcaatgtcg tgcatctcgg cgaactggcg catatcgttg
     7741 aggttcagcg catggctgaa gacggtatgg tgcgcgccac cagcgaggat ccacgcttcg
     7801 gaagcagttg gcagatccgg ttgcgctttc cacagcgcat tcgccaccgg cagtttcggc
     7861 agggagtgcg gtgttttcac cgtgtcgatg cagttaacca gtagacggta acgatcgccg
     7921 agatcaatca agctggcgac aatcgctgga ccggtttggg tattgaagat caggcgggca
     7981 ggatcgtcct taccaccaat accgagatgc tgaacgtcga ggatcggttt ctcttctgcg
     8041 gcgatcgacg ggcagacttc cagcatatgg gagccgagca ccaggtcatt gcctttctcg
     8101 aagtgatagg tgtagtcctc cataaaggag gtgccgccct gcagaccggt tgacatcacc
     8161 ttcatgatgc gaagcagggc ggcagttttc cagtcgcctt cgcccgcaaa gccgtaaccc
     8221 tgctgcatca gacgctgtac ggccagacca ggaagctgtt tcagaccgtg caaatcttca
     8281 aaggtggtgg tgaacgcgtg gaagccacct tgttccagga aacgtttcat ccccagctca
     8341 atacgcgccg cttccagcac gttctgtcgt ttttcgccgt ggatttgtgt ggcaggcgtc
     8401 atggtgtagc agctttcgta ctcatcgacc agcgcgttaa catcgccgtc gctgatggag
     8461 ttcaccacct gcaccagatc gccaaccgcc caggtattga cggagaaacc gaacttgatc
     8521 tgtgcggcaa ctttatcgcc atcggtgacc gccacttcac gcatgttatc gccaaatcgg
     8581 cagactttca gatgacgggt atcctgttta gataccgcct gacgcatcca ggagccgata
     8641 cgctcatggg cttgtttatc ctgccagtga ccggtaacca cggcatgttg ctgacgcata
     8701 cgcgcgccaa tgaagccgaa ctcgcgaccg ccgtgcgcgg tctggttcag gttcataaag
     8761 tccatatcga tgctgtccca cggcagcgcc gcgttgaact gggtgtggaa ttgcagcaac
     8821 ggtttgttga gcatggtcag gccgttgatc cacattttgg ccggggagaa ggtgtgtagc
     8881 cacaccacca gaccagcgca acgatcgtcg taattagcat cacggcaaat agcggtgatt
     8941 tcatccggcg tggtgcccag cggtttcagt accagtttgc agggcagttt cgcttccgta
     9001 ttcagcgctt tcacaacgtg ctcggcatgt tgggtgacct gacgcagggt ttccgggcca
     9061 tacagatgct ggctgccaat tacaaaccac acttcataat tatcaaaaat cgtcattatc
     9121 gtgtccttat agagtcggaa cggcctgggc agcctgtgcc ggggcggaag ttggaagata
     9181 gtgttgttcg gcgctcatcg cccattgctg atagcggcga taaagctgtt caaagcgttg
     9241 tgcctgttcg ctgcgcggtt gcagggtttt ctctaccgca ctggccattt tttgctgggc
     9301 tgatgggatg tctgcgtgca ctttcgcggc gacggcagca aaaatcgccg caccgagcgc
     9361 acagcattgg tcagaggcaa caatttgcag cgggcgattc agcacgtcgc agcaggcctg
     9421 cataatgacc tggtttttcc gcgcgatgcc gcccagcgcc atcacgttat tgacggcgat
     9481 cccctgatcg gtaaagcact ccatgattgc gcgtgcgcca aaggcggtgg cagcaatcaa
     9541 accgccgaac agcagcggag cgtcggtagc gaggttaaga tcggtaatca cccctttcag
     9601 gcgttggtta gcgttcggtg tgcggcggcc gttaaaccag tcgagcacca ccggcaggtg
     9661 atccagagac ggatttttgg cccatgcttc ggtcagcgcc ggaagcagtt gtttctggct
     9721 ggcgtcgatt tgctctttta gttccggatg ctgggcggca agctgttcca gcggccagcc
     9781 gagtacgcga ccgaaccagg cgtagatatc accaaacgcc gattggcctg cttccagacc
     9841 gataaatcca ggcaccacgc tgccatcaac ctgaccgcaa atacctttca ctgcccgctc
     9901 gccaacgctc tgtttgtcgg caatcagaat gtcgcaggtg gaagtaccga taacttttac
     9961 cagtgcgtta ggctgtgcgc ctgcaccaac tgcgcccata tggcagtcaa acgcgccgcc
    10021 ggaaatcacc acgctttcag gcaggccgag acgctgcgcc cattccgggc ataaggtgcc
    10081 caccggaata tcggcagtcc aggtatcagt gaacagcggg gaaggcaaat ggcgattgag
    10141 gatcggatcc agctcatcaa agaaactggc tggcggcagg ccgccccagc tttcgtgcca
    10201 tagcgactta tgcccggcgc tgcaacgtcc gcgacgaata tcctgcgggc gcgtggtacc
    10261 ggaaagcaga gctggcaccc agtcgcacag ctcaatccac gatgcggcag attgcgccac
    10321 ggcgctgtcc tggcgagtca catgcaggat ttttgcccag aaccattcgc tggaataaat
    10381 accgccaata tagcgggagt agtcaacatt gcccggcgcg tggcacaaac gggtaatctc
    10441 ttccgcttct tcaaccgcag tgtggtcttt ccacaatacg aacatcgcgt tcgggttttc
    10501 ggcaaactcc gagcgcagcg ccagcacgtt accgtcggca tcaatcggtg cgggcgtcga
    10561 gccggtactg tcaacgccaa tcccgaccac agctgcgcgc tgttcgacgc taagttctgc
    10621 aagcacggtt ttcagtgccg cttccattga ctcaatgtag tcacgcggat gatgacggaa
    10681 ctggttattc ggggcatcac aaaattgccc tttttgccaa cggggatacc actctacgct
    10741 ggtggcgatt tcttcaccgg tggtgcagtc caccgccaaa gctcgcacag aatcactgcc
    10801 aaaatcgagg ccaattgcaa tcgccatcgt ttcactccat ccaaaaaaac gggtatggag
    10861 aaacagtaga gagttgcgat aaaaagcgtc aggtaggatc cgctaatctt atggataaaa
    10921 atgctatggc atagcaaagt gtgacgccgt gcaaattatc aatgtggact tttctgccgt
    10981 gattatagac acttttgtta cgcgtttttg tcatggcttt ggtcccgctt tgttacagaa
    11041 tgcttttaat aagcggggtt accggttggg ttagcgagaa gagccagtaa aagacgcagt
    11101 gacggcaata tctgatgcaa tatggacaat tggtttcttc tctgaatggc gggagtatga
    11161 aaagtatggc tgaagcgcaa aatgatcccc tgctgccggg atactcgttt aacgcccatc
    11221 tggtggcggg tttaacgccg attgaggcca atggttatct cgattttttt atcgaccgac
    11281 cgctgggaat gaaaggttat attctcaatc tcaccattcg cggtcagggg gtggtgaaaa
    11341 atcagggacg agaatttgtc tgccgaccgg gtgatatttt gctgttcccg ccaggagaga
    11401 ttcatcacta cggtcgtcat ccggaggctc gcgaatggta tcaccagtgg gtttactttc
    11461 gtccgcgcgc ctactggcat gaatggctta actggccgtc aatatttgcc aatacgggtt
    11521 tctttcgccc ggatgaagcg caccagccgc atttcagcga cctgtttggg caaatcatta
    11581 acgccgggca aggggaaggg cgctattcgg agctgctggc gataaatctg cttgagcaat
    11641 tgttactgcg gcgcatggaa gcgattaacg agtcgctcca tccaccgatg gataatcggg
    11701 tacgcgaggc ttgtcagtac atcagcgatc acctggcaga cagcaatttt gatatcgcca
    11761 gcgtcgcaca gcatgtttgc ctgtcgccgt cgcgtctgtc acatcttttc cgccagcagt
    11821 tagggattag cgtcttaagc tggcgcgagg accaacgcat cagccaggcg aagctgcttt
    11881 tgagcactac ccggatgcct atcgccaccg tcggtcgcaa tgttggtttt gacgatcaac
    11941 tctatttctc gcgagtattt aaaaaatgca ccggggccag cccgagcgag ttccgtgccg
    12001 gttgtgaaga aaaagtgaat gatgtagccg tcaagttgtc ataattggta acgaatcaga
    12061 caattgacgg cttgacggag tagcataggg tttgcagaat ccctgcttcg tccatttgac
    12121 aggcacatta tgcaagcatt gctggaacac tttattaccc aatccaccgt gtattcattg
    12181 atggcggtgg tgttggtggc ctttctggag tcgctggcgc tggtcggttt gattctaccc
    12241 ggtacggtgc tgatggcggg gctgggagcg ctgattggca gcggcgagtt aagtttctgg
    12301 cacgcctggc tggcagggat cgtcggttgt ctgctgggcg actggatctc cttctggctg
    12361 ggctggcgtt ttaaaaagcc attgcatcgt tggtcatttc tgaagaagaa caaagcatta
    12421 ctcgataaaa ctgaacatgc gttgcatcaa cacagcatgt tcaccattct ggtcggtcgt
    12481 tttgttggcc cgacgcgtcc gctggtgcca atggtggcgg gaatgctgga tctcccggtg
    12541 gcaaaattta tcacgccgaa tattatcggc tgcctgctgt ggccaccgtt ttacttcctg
    12601 ccggggattc tggcgggtgc ggcgatcgat attccagccg gaatgcagag cggtgagttt
    12661 aaatggttgc tgctggcgac ggcggtattt ttgtgggttg gtggctggct gtgctggcgg
    12721 ttatggcgca gcggcaaagc aaccgatcgt ttgagtcatt atttgtcccg cggtcgtttg
    12781 ttgtggctga cgccgttgat ttctgccatc ggcgtggtgg cgctggtggt gttagttcgc
    12841 catccgttaa tgccggtgta tatcgatatt ttgcgtaaag tggttggagt ttagggcata
    12901 gtcttgtgtg gggcgcttga tgcggacaaa acgcacccgt aggtcggata aggcgttcac
    12961 gccgcatccg acagtgcata ttaacctgta attcccaaca gcgccgaagc actcgcctta
    13021 ccgctcaaca actcattggt cataccctgc caggcgattc gcccgtcggc gactactacc
    13081 gagcgcgtgg caatccgcgc cgcatcttcc acactgtgcg acaccatcaa tagcgtcatt
    13141 ttttgctgct ggcagctagt gctcaccagc gtcaacatct cctgacgtag cgccggatcg
    13201 agcgcagaga acggttcatc gagcaataaa atcggctgtt cgcgtaccag acaacgcgct
    13261 aacgccacgc gctgtcgctg accgccggaa agctcgcccg gtaaccgcgc cattaaatta
    13321 tcaatcccca tctgacgggc aatcgcgtgc attttctcct gctgtaccgc gttcagcttc
    13381 aatcctggat taagccccag cccgatgttc tgcgcgaccg tcaggtggct gaacaggttg
    13441 ttctcctgaa acagcatcga caccggacgg cgtgacggcg gcgtcgtggt gtgatcaacg
    13501 ccgtcgatag tcagcgcacc gctggctggt gtcagaaaac cggcgatcaa gttcagcagg
    13561 gtacttttac ccgcgccgct tggcccgagg atcgccacct gctcgccgcg ttctaccgtt
    13621 aagctaaaac gcatcggcaa atggtgataa agccaggtga tatcagtcag ttttaacatt
    13681 tcgccccggt attttttcaa tcacgctaaa cagcagaaaa cagagcagca gcagaattaa
    13741 cgcggtgacc gcgccgtcct ggctgcgata ggagccaatt tgctggtaga gataaaacgg
    13801 cagggtgcgg aaatcatcgt taccgaacaa cgccaccacg ccaaaatcac caatcgacag
    13861 cacgcaggca aaggccagcg cctgcgccag cgggcgtttc agggcgcgca gctccactac
    13921 ttttaagcgt gaccagcctt caatccccag cgactgacat aacatgctgt agcgggcggt
    13981 gatatcgcgc atcgggtttt ccagcacttt cagggcgtag gggatcgcca ttaacgcatt
    14041 ggtgaaaatc acaatgccgt cagcagattg tggcaggccg atagtgttgt tgagcagtaa
    14101 aaagaagccc gttgccagca caatccccgg catagcgagg atcaacatgc cgctcatctc
    14161 cagcgcctgg cccgccagca ttttttgccg cgcccgcagt tcgcgactgc tccatagcag
    14221 catcatggtc agcactacgc acaatacacc tgccgccagc gcaatacgca acgaggtcca
    14281 cagcgcctgc cacagcaccg gttgtgccag cacttccggc aactggcgat ttaccccatc
    14341 gacgatcacc gccagcaacg gtggcagcaa cagcagcagc gccagcacaa ttaacatcgt
    14401 gtcgcaaatg cggctatgca gacgatcgtc cgggtcgcgc cagccttgca gcagcgtggt
    14461 gccgggcgca atggccttac tcagtcgctg actcaacagc accagcccaa ggcaacacac
    14521 catctggatc agcgccagca tcgccgcgcg ggcaggatcg tagtcgtaac tcagcgcctg
    14581 atagattgcc agctcgatag tggttgcctg cggaccgccg cccagcgata acacggtggc
    14641 gaagctggcg aaacagagca taaagataag cgcggtaacc ggcgggattt gtcgccgtaa
    14701 ccacggccat tcgacgaagc ggaaaaaatg ccagccgcgc atcccaagct gggcggcaag
    14761 ctgacgttgt tcgccgggaa tgttttccag tgcctggagt aataagcggc tcgccatcgg
    14821 cagattaaaa aacacatggg ccagcaaaat gccttgcagg ccgtagggcg aaaaggtcca
    14881 ctccagaccg agcgattggc agagcgatgc cagccagccc tggcgaccat agacgctaag
    14941 aatgccgaaa acagcaacca gcaccgggag gatcaaggtc attgcgcaca gacgcaacag
    15001 cgccagccga cccggaaagc gcctgcgata gagcgcgcgg gcgaggaata tcgcgggtac
    15061 gacagagagt agcgccgaga gaaacgcctg ccagaaggag aagcgcacca catgccacag
    15121 gtagctgtcc tgccagactg ccgaccagtc accctgcggc gcgttccacc acagggcgag
    15181 aaacgccgcc agcgcaaccg ctaccaccag cgtggcggcg cttacacctg gaattaacca
    15241 gccgggaatt aacggctgac ggcgcgttgc cattcgctaa tccatgcctg acgttgtgcc
    15301 gccacttcgg ctggcgtgaa ctccagcgtg gttgcgggtt tggtcaattt ttcaaaaccg
    15361 gcaggcagcg tgacgtttgc caccggatac atccagttgc cggttgggat cgcattctgg
    15421 aaagccggag aaaccataaa ctggaggaat ttttgcgcca gctccggctg cttgctggca
    15481 gcggtgcgag cggcgacttc cacttgcaga tagtgacctt cgctgaagtt cgcggcggcg
    15541 tagttatctt tcttctcttc gagaatgtga taagccggag aggtggtgta actcagtacc
    15601 agatcgcttt caccttttaa aaacaggccg taggcttcgc tccagccttt ggtgaccgtg
    15661 accgttttct tcgccagttt ctgccaggct tgtggggcgt catcgccata gactttttgc
    15721 atccatagca acagacccag ccccggtgta ctggtgcgcg gatcctgata aatcacccgc
    15781 cagttttgat cgctctcaac cagttctttc aggctttgtg gcgggttttt cagtttgttc
    15841 ttgtcataaa cgaaggcgaa gtagccataa tcaaacggta cgaaaatgtc attattccag
    15901 ccgccgggaa cgttaacggc atccgctgcc acaccgcttt tggcaaacag tccggtttta
    15961 ctggcggcgt ctaacagatt gttatccagc cccagcacca catcggcttt actgtttttg
    16021 ccttccatcc gtagacggtt gagaagcgaa acgccatctt ccagcgccac cagtttcagt
    16081 tcgcaattac agtcggcttc aaaggctttt ttaaccaccg gaccaggccc ccagtcggcg
    16141 gcgaaggaat cgtaggtata aacagtcaga acgggtttag cgaaaacggg cgctgtgcac
    16201 agcaacagca ggggaagaca tttttttaac actttgcacc tcaaaaaaga gtggcaaagg
    16261 acttgagaag gagcctcaaa tcccttcgcc ggcgttatcc ggatcaggtt cgacgggtat
    16321 tttctcagcg cacgcgtccg cgtggcaccc cgttgagaac ggcgttagtg tagtgatttt
    16381 gttatcaacc agcaatccat agagaaggcg tattaaaaaa gccaatattc gttcagctat
    16441 caagagcgta tttgcatagc tggagagcgt tgatccgaaa gaatcccttc gctacagtca
    16501 aaataggtaa cgtaaccgtt tattgcgtga atgagcgatt tttgacagta ttcttattca
    16561 ggacgcttat tctgagtttt ttattcagag tggagctatc agcatgaaag taagaaaccc
    16621 agaacagatt agtatccctg ctagtaatac cactaaagat ccaggcctta ctaattctca
    16681 gattatcagg atggctaact tggtcaaaaa gaccgagaat atgaatatct ttgaagagct
    16741 atgggagacg ttacgcaatc tcttccagtc agataaacat tcccagactg cggcaaggca
    16801 aattctgaag gatgcatttt atttccagaa tagtgatgac tactcaaaat actttaccgg
    16861 ggctgtcgac gggaaggcgc gtgataagtt aactcactgg ctgataaaat ttaatgaact
    16921 caaagaatac gcaaaagatc cggaaaatat ggccgcgaag gcctcactct cgccagaagg
    16981 tacgttatgc gtcagttttt ttattggcga tgaggcgata tttactctgg agttgcagct
    17041 gaaaaagagc accaggaccg gggggattga tctgagtaat gcttacttta acggggttgt
    17101 catttgtggt attgactgtc tcgaggttga cctgagcaat gctgaaacga acaatagccg
    17161 ttggtatgac tgacctgcgc gaggttaacc tgaaaaagct gacctttccg gccagaacaa
    17221 ggctagactg cgccgttatt gattaactca gaatatgaaa gaggggctgg aatgcccctt
    17281 ctttaatctt atttgcgacg ctaatcagac gatgcataaa acagagaaaa tataaaagta
    17341 atgtatttta tcattccgtc tgaatataaa aaatgcgctc atgaccgcca aaaggcgcaa
    17401 gctactctgg atccggcggc gcaaaccacg ctgatttaaa atcaaaccag ccgagggtgt
    17461 tcatgcgcag gccgcgcata ctgcgttgtc cctgaatgat cagccagtgg tggataagtg
    17521 gcaccattgc tttgctggcg accagttgtt ggcaccagtt cgccaggttc atttcgccat
    17581 tgcgccagcg ggcggcgtcg acttgccagt cgattggaat acagtgttgg agcagcggta
    17641 cctcgcacag gtgcgcgaac agcgaaaaat ccagcggcag cgtaaagttg gcgctgttaa
    17701 gccagatatc gctctcgatc tctccttcat gccactgatc gtagctgatc tctttgattt
    17761 ccagtgtgac ctggtggctt gccagaatct gctgcatgat cccggcaatc acccgatgct
    17821 cactgtgatc ctgataaaag gtcagggtga ggctttccag accagccggt ttttcgctcg
    17881 ttatggtgcg ggcatgatgc caacggggga gcagtccata agccgggaac cacagttgct
    17941 ggtactgttc ctcagcgaaa tagaccagat tagttggaga aagcacatag cttacccagt
    18001 ccctgacttg ctgattcgcc ccgcgatggg tgcggctatc gaacagtaaa tagtagcaac
    18061 cttcctccag gcggctttca atctcttttt cctcgccctg tggacctttt agcatcagcc
    18121 ctccggctgg ctcgtcggca atttccggca gaacccagac gttaacttca tcgattaatg
    18181 cccggtaacc gaagaagtca tcgaatgcct gaattttcag ttgattggtg ctgttgcgaa
    18241 tcaccgcata cggaccggtg ccgatgggat ggctggcaaa gttactgagg gtttcccatt
    18301 cgcgcggcag gatcatcgcc ggaacttgtc ccagcagtaa cggcaaccag cgatccggct
    18361 gcgtgaggtg gatatccagc gtccagggcg tcggcgacac aatgtcagca atatgcgaat
    18421 agagcggcag cgtattgatt cgttttaaag aggcgatcac gtcgtccatt tccagttcac
    18481 gaccatgatg aaaatggact cctggacgca aaaagaaacg ccagtgaagc ggtgatattt
    18541 gctgccagtg gtgggcgatg tctgcttcca gttccccatt ttcctcattt atgcgcgtta
    18601 gcgaactgaa gatttgccgg gcgatatggg tttcggaacg gcgcaatgcg ctgccaggta
    18661 gcagattacg caacggacga tagtagagca cgcgcaggat gtgccgcccc tggcggaagc
    18721 tgcggcccag atgagaaacc agcatttgcc tcacagtcgc tttgtcgcca actaactgca
    18781 ctagttggtc gatacggtcc tgctccagca ggtcttccgc ccgctgttgc tgaagcgcca
    18841 gcccggtata gaggaatgtc agacgcgagc gtttaccacg cccgacttcc gcttcccacg
    18901 tcagccagcc gcgatcctgc atggtgttga gcagggtacg catatgacga cgcgagcagc
    18961 tcaataacgc tgccagttcg ttgagcgttg tgtcctgcga tttaccctcg cagcattgcc
    19021 acaggcggat gaactgttgt tgcagacgag cagatggcat aaaaggggaa ctcctgcgca
    19081 aaagacagca attttatttt ccctatatta agtcaataat tcctaacgat gaagcaagga
    19141 ggtgccccat tcgtcagttt tatcagcact attttaccgc gacagcgaag ttgtgctggt
    19201 tgcgttggtt aagcgtccca caacgattaa ccatgcttga agaactgatg cagtgggagg
    19261 ggagtcattc tgactactga tttgcggaca tcatgtgtga ctgagtattg gtgttaatcg
    19321 ccacgccagc agtgattatc tgctggcttt tttctttttg gcgattgagt atcggacggt
    19381 gtacaaacta acgcataaac gcgggttgct tcgcttcata agccgcaatg gcgtcgtcgt
    19441 gctgcaaggt aagcccaatg ctgtccagac cgttcatcat gcagtggcgg cggaaggcat
    19501 caatggtaaa gcgataggtt ttctcacccg ctttcacctc ttgcgcttcc aggtctacat
    19561 caaaatgaat ccccggattg gctttcacca gcgcaaacag ctcatccact tctgcgtcgc
    19621 ttaatttcac cggcagcagc tggttgttaa agctattgcc gtagaagatg tcagcaaaac
    19681 tcggcgcaat caccacctta aaaccgtagt cggtcaatgc ccagggcgcg tgctcacgcg
    19741 aggagccaca gccgaagttt tctcgtgcca gcaaaatgga agcgccctga tactgcgaga
    19801 agttcagcac gaagtccggg tttggctgtt ggcctttttc atccagaaaa cgccagtcgt
    19861 taaacagatg cgcgccaaaa ccagtacggg tcactttctg caaaaactgt ttcgggatga
    19921 ttgcatcggt atcgacattg gcggcatcca gcggaaccac caggcctgtg tgtttgataa
    19981 atttctctgc catggtgtgc tccttattta atgttgcgaa tgtcggcgaa atggccggtc
    20041 acagccgcgg cggcagccat tgccgggctg accagatgcg tgcgcccgcc gcgcccctga
    20101 cggccttcaa agttacggtt gctggtggag gcacaacgtt cgcccggatt cagacggtcg
    20161 ttgttcatcg ccagacacat tgagcagcca ggcaagcgcc attcaaaacc ggcttcaata
    20221 aagattttat ccagaccttc cgcttccgcc tgcgctttta ccggaccaga gccgggaacc
    20281 accagcgcct gtacgcctgg cgcgactttc cgccctttgg cgatttccgc cgccgcgcgt
    20341 aaatcttcaa tacgtgagtt ggtgcaggaa ccgataaaca ctttgtcgat agccacttcg
    20401 gtcagcagaa tacccggttt cagccccata taggccaacg ctttttctgc cgacgcgcgt
    20461 tcaaccggat cggcaaacga agccggatcg ggaatattgt cgttcacgga aatcacctgg
    20521 cctgggttag ttccccaggt gacctgcggt gaaatctctt ctgcctgcaa agtgacaacg
    20581 gtatcgaaag ttgcgccttc gtcggtttgc agggttttcc agtaggcaac ggcgtcgtcg
    20641 aaatctttgc ctttcggcgc atgcagacgg cctttgacat agttaaaggt ggtttcgtca
    20701 ggtgcaacca gaccggcttt cgcgcccatt tcgattgcca tattgcacag ggtcatccga
    20761 ccttccatgc ttaaatcacg gattgcttcg ccgcaaaact ccaccacatg cccggtgccg
    20821 cctgcgctac cggttttacc gataattgcc aacacgatat cttttgcggt aatgcccggc
    20881 gcggctttgc cctggacttc aattttcatg gtcttcgcac ggccctgttt cagggtttgc
    20941 gttgccagta cgtgttcaac ttcggaagtg ccgataccaa aggccagtgc gccaaacgcg
    21001 ccgtgggtgg cggtatgcga gtcgccgcag acaatggtca tccccggcaa ggtcacgccc
    21061 tgttccggcc ccattacgtg gacgatccct tgataggggt gattcaggtc atacagttcg
    21121 acgccaaatt ctttgcagtt tttgatcagc tcctgcatct ggatgcgcgc catttcaccg
    21181 caggcattaa tgtctttagt ctgggtagag acgttgtgat ccatggtggc gaaggttttg
    21241 cccggctgac gtaccgggcg accgtgggcg cgcagaccat cgaacgcctg cggtgaggtc
    21301 acttcatgca ccaggtggcg gtcgatatat aacagcgggg tttcgttttc ggcttcgtac
    21361 actacgtgag cgtcgaataa tttttcgtat aacgtcttag ccatgattac accccttctg
    21421 ccacatagcg ggcaatgata tcgcccattt catcggtact aacggcggca gcgccacggg
    21481 ctaaatcccc ggtgcgaatg ccttcttcta atgcgcggtt aatggcgcgt tcaatggcgg
    21541 aagccgcatc atcggcatcc aggctgtaac gcagcagcag tgccagcgac agaatttgcg
    21601 caatcgggtt ggcgatgttt ttacctgcga tatctggtgc cgagccgccc gccggttcat
    21661 acagtccaaa accttgctcg ttcaggctgg cggaaggcaa catccccatc gagccagtga
    21721 tcattgcgca ctcgtcagac agaatgtcgc caaacaggtt ggagcacagc agaacgtcaa
    21781 actgtgatgg atctttaatc agctgcatgg tggcgttgtc gatgtacata tgcgccagtt
    21841 cgatatccgg gtattccgtg gcgatctcgt tgacgatttc acgccataaa atagaggatt
    21901 gcagcacgtt ggctttatcg atcgacgtca ctttatggcg acgtttacga gcagattcaa
    21961 acgcgatgcg ggcaatacgc tcgatctcaa aacggtgata cacctcggta tcaaacgctt
    22021 tttcatattg tccgctacct tcgcggcctt ttggctgacc gaaatagatg ccgccggtca
    22081 gttcgcgcac gcacaggatg tcgaagccgt ttgcggcaat gtctgcacgc agcggacaga
    22141 atgcttccag cccctgatac agttttgccg gacgcaggtt gctgaataat ttgaagtgct
    22201 tacgcaaagg caacagcgcg ccgcgttctg gttgctggtc cggtggtaaa tgttcccatt
    22261 tcgggccgcc taccgagcca aacagcacgg catcggcttg ctcacaacct tcaaccgtcg
    22321 caggcggcag tggttgcccg tggttatcaa tggctgcgcc gcctacatcg taatggctgg
    22381 tagtgatgcg catcgcaaag cggttgcgca cggcatccag cactttcagc gcctgggtca
    22441 tcacttccgg accaatgcca tcccccggca atacggcaat atggtaattc ttcgacatca
    22501 cacggtttcc ttgttgtttt cgttgtgttg agctttgcgt tgcaactctt tttcgacttc
    22561 tgtggcacgc cagatattgt taagtacgtg caccatggct ttggcggagg actcgacaat
    22621 atcggtggcc aggccgacgc cgtggaagcg gcgaccgttg tagttggcga caatatccac
    22681 ctgacccagc gcatctttac cgtgaccttt ggcggtcagg ctgtatttca ccagttcgac
    22741 gttatagtca gtgatgcggt taatcgcctg atagacggca tcgaccggac cgttaccgtt
    22801 ggcggcttct gctttgactt cttcgccgca ggccagtttg acggcggcgg tggcgatatc
    22861 gttagagcca gactgcacgc tgaagtaatc cagacggaaa tgctccggct cttcttgctg
    22921 cttaccgatg aaggccagcg cctccagatc gtaatcaaac acctgacctt ttttgtccgc
    22981 cagcttgagg aaggcgtcgt acaaattgtc taaattatat tcactttctt tataccccat
    23041 ctcatccatg cgatgtttca ccgccgcacg ccccgaacga gaggtcagat tcagctggat
    23101 ttggttcaga ccaatagatt ctggtgtcat gatttcgtag ttttcgcggt ttttcagcac
    23161 gccatcctgg tggataccgg aggagtgtgc gaatgcgccg ctgccaacaa tggctttgtt
    23221 tgccgggatc ggcatattac aaatctggct aactaactgg ctggtgcgcc atatctcctg
    23281 gtgattaatg gcggtgtgga cgttgagaat atccttacga actttaatcg ccatgatgac
    23341 ttcttccagg gaacagttac cggcacgctc gccgatcccg ttcattgcac cttccacctg
    23401 gcgtgcaccg gcatgtaccg ccgccagtga gttgccgacc gccaggccca aatcgtcgtg
    23461 ggtatgtacg gaaataatgg ctttgtcgat gttaggcacg cgttcataca ggccgctaat
    23521 gattccggcg aactcaaacg gcatggtgta gcccacggtg tccggaatgt tgatggtggt
    23581 ggcaccggca ttaatcgccg cttcgaccac tcgcgccaga tcggcaatgg gtgtacgccc
    23641 ggcatcttcg caagaaaatt caacatcatc ggtgtaatta cgggcgcgtt tcaccatata
    23701 gatagcgcgt tcgatcacct cgtccagcgt gctgcgcagc ttggtggcga tgtgcattgg
    23761 cgaagtggca ataaaggtat gaatacggaa ggcttcggcg actttcaggg attcggccgc
    23821 cacgtcgata tctttctcca cgcagcgagc taacgcgcat acacggctgt ttttaacctg
    23881 gcgagcgatg gtttgcactg attcaaaatc acccggcgaa gagacgggga acccgacttc
    23941 catcacgtca acacccatac gctcaagggc cagcgcaatt tgcagttttt ctttcacact
    24001 caagcttgcc tgtaacgcct gttcaccgtc gcgcaatgtg gtatcgaaaa taatgacttg
    24061 ctggctcatg gtttgggtcc ttgtctcttt tagagcgcct cgcttcgggc ataaaaaaac
    24121 ccgcgcaatg gcgcgggttt tttgtttgac tgcgtgctgg cttaatgctg gatgccgctc
    24181 actcgtctac cgcgcaaaga agatgcgttt agtagtagta gtagaccgat aaagcgaacg
    24241 atgtgagtca ttaaatcagc tccagatgaa tgcgatatgc ttttagagtt actggataca
    24301 aaaacggatg tcaaccctga cgcaataaaa acgtcccgcc agcatgagtt ctgcatccgt
    24361 aagattagct aattgtgctg ctgtggttaa aacaagcgat attaatttct gcttaactac
    24421 cgatgctttt catcggttga catatttcag aataaatttt tgcatctaat ccacgaggaa
    24481 aaaggggaca aaatgcacgc gttgcaaaac ctatcctgat gatttgtatt gaattatatg
    24541 ttttgcggat tttttgatat tgatttggtg aatattattg atcaattaat gttaagaatt
    24601 aatgcattaa a
//
LOCUS       DAZKIP010000064        24049 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZKIP010000064 DAZKIP010000000
VERSION     DAZKIP010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.064"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            116..3868
                     /gene="yfaL"
                     /locus_tag="ACHOUK_002803"
     CDS             116..3868
                     /gene="yfaL"
                     /locus_tag="ACHOUK_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFP9898215.1"
                     /translation="MRVIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQVWSIADGQWLVFSDMTNNASGGAVFLQQGAEFTLSPENETGMTLFAN
                     NTVSGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAIDLRVTNA
                     VFRNNIANDGKGGAIYTINNDIYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSSAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQN
                     QAELNVGSTQQTFAHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLAGAQSMALTGDIVVDAGAVLSLEGDAADLAALQDDPQSIVLNGGMLDLSDFSTWQS
                     GTSYKDGLEVSGSSGTVIGSQDVVDLAGGNDMHIGGDGKDGVYVVIDAGDGQVSLAND
                     NQYLGTTQIASGTLMVSDNSQLGYTHYNRQVIFTDKPQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSA
                     VRVEEGTLQGDVADIFPYASSLWVGDGATFVTGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNCDGTLVNATDGVTLTGELNTNLETDSLTYLSNVTVNGNLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPSPPDPDPTPDPDPTQDPDPTPDPEPTPAYQPVLNAKVGGYLNNLRAANQAF
                     MMERRDHAGGDGQTLNLRVIGGDYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGTDGEW
                     MLGIVGGYSDNQGDSRSSMTGTRADNQNHGYAVGLTSSWFQHGKQKQGAWLDNWLQYA
                     WFSNDVSEHEDGVDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAA
                     NRARVSQSQGDDIQTRLGLHSEWRTAVHVIPTLDLNYYHDPHSTEIEEDASTISDDAV
                     KQRGEIKVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(4009..4731)
                     /gene="ubiG"
                     /locus_tag="ACHOUK_002804"
     CDS             complement(4009..4731)
                     /gene="ubiG"
                     /locus_tag="ACHOUK_002804"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFP9898216.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LSYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNTFKLGPGVDVNYMLHTQNK"
     gene            4878..7505
                     /gene="gyrA"
                     /locus_tag="ACHOUK_002805"
     CDS             4878..7505
                     /gene="gyrA"
                     /locus_tag="ACHOUK_002805"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFP9898217.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7713..9341
                     /gene="yfaA"
                     /locus_tag="ACHOUK_002806"
     CDS             7713..9341
                     /gene="yfaA"
                     /locus_tag="ACHOUK_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFP9898218.1"
                     /translation="MALLSAGVWALQYAGSGPEKTLSPLVVHNNLQIDLNEPDLFLDS
                     DSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIEGSIRRIVYEHDLTLKDKLFS
                     SLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPLLFAATSDSQLSKTEISSIKI
                     NSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFKDDQQDTEATAIASDLLSGKK
                     RWQASFGLEERAAEKTPVRQRIVASARLLGFGYQRLMPSFAGVRFEMGNDGWHSFVAL
                     NDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAEEMLSHISQENDKLNGALDGA
                     AGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNIGAHESKAPEGVLPVSQTQQG
                     EAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLAMQNKTLLFSLDDTLVNNALQ
                     TLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLTSLPKNLEPVFYNAAQTLLMP
                     KLDALSQQPRYVMKLAQMEPGAAWQWLPIIWQPL"
     gene            9338..9961
                     /gene="yfaT"
                     /locus_tag="ACHOUK_002807"
     CDS             9338..9961
                     /gene="yfaT"
                     /locus_tag="ACHOUK_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFP9898219.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            9985..14499
                     /locus_tag="ACHOUK_002808"
     CDS             9985..14499
                     /locus_tag="ACHOUK_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HFP9898220.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEESKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELQLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGKELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVDVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGDLPSGGKSFTVNFDKPGNYNLTLRDKDGLILAGLSHAVSGKGSM
                     SHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPSAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGDNALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKTEDFSEEDTRDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAVNSNQPLAHTVVLLNSGGDATQAAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPSVVLPAPAGAWAKHKLTGGGEYWRWVGQGVP
                     DILSFGDELSPQNVQVRWREAAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAGKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            14500..16149
                     /gene="yfaQ"
                     /locus_tag="ACHOUK_002809"
                     /pseudo
     CDS             14500..16149
                     /gene="yfaQ"
                     /locus_tag="ACHOUK_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
     gene            16154..16930
                     /gene="yfaP"
                     /locus_tag="ACHOUK_002810"
     CDS             16154..16930
                     /gene="yfaP"
                     /locus_tag="ACHOUK_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFP9898221.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            17205..20054
                     /gene="rcsC"
                     /locus_tag="ACHOUK_002811"
     CDS             17205..20054
                     /gene="rcsC"
                     /locus_tag="ACHOUK_002811"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFP9898222.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKADPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLMIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(20254..20904)
                     /gene="rcsB"
                     /locus_tag="ACHOUK_002812"
     CDS             complement(20254..20904)
                     /gene="rcsB"
                     /locus_tag="ACHOUK_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFP9898223.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(20921..23593)
                     /gene="rcsD"
                     /locus_tag="ACHOUK_002813"
     CDS             complement(20921..23593)
                     /gene="rcsD"
                     /locus_tag="ACHOUK_002813"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFP9898224.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         5882 a         5850 c         6547 g         5770 t
ORIGIN      
        1 aaataataac aaaatagcta ttgcatcggg ttggacaatt tacaaaagaa agataaccca
       61 tattgtataa tgcgtttaaa tatgccgtct tatataaaat gatggtcaga ttaatatgcg
      121 ggttatcttt ctacgcaagg agtatttatc tctactcccg tcaatgattg catctctttt
      181 ctctgctaac ggtgtcgcgg cggccattga tttatgccag ggatatgata tcaaagcgag
      241 ttgtcacgcc agcaggcaaa gcctttcagg cattacgcag gtctggagta ttgccgatgg
      301 gcaatggctg gttttttcgg atatgaccaa taatgccagc ggtggggccg tatttttgca
      361 acaaggagcg gaatttacat tatcaccaga aaatgaaact ggaatgactc tgtttgccaa
      421 taacaccgtt tcaggagaat ataataacgg cggggcaata tttgctaaag aaaactcaac
      481 gctgaatctt acggatgtta ttttttctgg taacgtcgca ggcggctatg gtggcgcaat
      541 ctattcttct ggtactaacg ataccggtgc catcgattta cgtgtcacta acgccgtgtt
      601 tcgcaataac atcgctaatg acggcaaagg tggtgcaatt tataccatca ataatgatat
      661 ctatttaagt gatgatgttt ttaacaataa ccaggcatat acatcaacaa gttacagtga
      721 tggcgatggc ggcgcaatcg atgtcacaga taataatagc gacagcaagc atccttcagg
      781 ttatacgata ataaataaca ctgcctttac aaataacact gccgaaggtt atggcggggc
      841 gatatatacc aatagcgcga cggctcccta tcttattgat atttctgttg atgacagcta
      901 cagccagaac ggaggcgtgt tagtcgatga gaacaatagc gcagcaggct atggagatgg
      961 tccttcctct gcggcgggtg gctttatgta tctcggctta agtgaagtta cctttgatat
     1021 tgccgacgga aaaacgctgg ttattggcaa tacagagaat gacggagctg ttgactctat
     1081 tgctggtacc gggttaatca ccaaaacagg ttccggcgat ctggtactta atgcagataa
     1141 caatgacttt actggtgaga tgcagattga aaacggtgaa gttaccctgg gccgcagcaa
     1201 ctccctgatg aatgtcggcg atacgcattg ccaggacgat ccgcaagact gctacggtct
     1261 gacgataggg agtattgata agtaccagaa tcaggcagag ctaaatgttg gctccaccca
     1321 acaaaccttt gcgcactcat tgacgggctt tcagaatggc actttaaata tcgatgctgg
     1381 tggcaatgtt actgttaatc aaggcagttt tgctggcacc atcgaaggtg ctggtcagct
     1441 caccattgcg caaaacggca gctatgtgct ggcgggggcg cagtcgatgg cgctaaccgg
     1501 cgatatagtg gtggatgctg gtgcggtgct ttcgctggaa ggcgacgcgg cagatcttgc
     1561 cgctctccag gacgatccgc agtcgatcgt gttaaacggc ggtatgctcg atctctctga
     1621 tttctccacc tggcagagcg gtacatcata caaagatggc cttgaagtca gtggcagcag
     1681 cggaacggtt atcggcagtc aggatgtggt agatcttgca ggcggaaacg atatgcatat
     1741 cggcggcgac gggaaagatg gcgtctacgt ggtgatcgat gcgggtgacg ggcaggtcag
     1801 cctggcaaat gacaatcaat acctcggcac aacgcaaatc gcttccggta cgctgatggt
     1861 gagcgacaac tcgcagcttg gatataccca ttataaccgc caggttatct ttaccgataa
     1921 gccacaagaa agcgtgatgg agattactgc caatgtcgat actcgctcta caacgactga
     1981 gcatgggcgt gatattgaaa tgcgcgccga cggtgaagtg gcagttgatg cgggggtaga
     2041 cacgcagtgg ggcgcactga tggctgacag cagcgggcag catcaggatg agggtagcac
     2101 attgactaaa acgggggcgg gtacactgga gctgaccgcc agcggtacaa cgcagtcagc
     2161 ggtgagagta gaagagggca cgctgcaagg tgatgttgcg gatatcttcc cttatgcttc
     2221 gtcgctatgg gtcggtgacg gggcaacgtt cgttactggc gcggatcagg atattcagtc
     2281 aattgatgct acttccagcg gcactatcga catcagcgat ggtacggttt tgcgcctgac
     2341 cgggcaggat acttccgtcg cccttaatgc ctcactgttt aactgcgatg ggacgctggt
     2401 gaatgccacc gatggtgtga cgttgacagg tgagcttaat accaaccttg aaactgacag
     2461 cctgacttat ctttccaacg tgacggttaa tggcaatctg accaatacgt ccggtgcggt
     2521 tagcctgcaa aatggcgtcg ctggcgatac gctgacggta aacggtgatt ataccggcgg
     2581 cggtacgcta ctgctcgata gcgaattaaa cggcgatgac tcggtaagcg atcaattggt
     2641 gatgaacggt aatactgctg gcaacacaac tgtggtggtt aactccatta cagggattgg
     2701 tgagccgaca tcgacaggca ttaaagtggt tgatttcgca gctgatccca cgcagtttca
     2761 aaacaatgcg cagttcagtc tggcaggcag cggctacgtc aatatgggag cgtatgacta
     2821 cacgctggtg gaagataaca acgactggta tctgcgatcg caagaagtaa cgccgccatc
     2881 gccacctgat ccagacccga ctcccgatcc tgatcccacg caggatcctg atccaacacc
     2941 cgacccggaa cctacgcctg cttaccagcc ggtgttgaat gccaaagttg gcggttatct
     3001 caataacctg cgggcggcaa atcaggcgtt tatgatggag cgacgcgatc acgcaggtgg
     3061 cgatggtcag acgctgaatt tacgtgttat cggcggagat tatcattaca cagcagcggg
     3121 gcaactggct caacatgaag acacttctac ggtgcaactt agcggcgatc tgtttagcgg
     3181 gcgctggggc acggatggcg agtggatgct tgggattgtt ggtggctaca gcgataacca
     3241 gggcgacagc cgctcgagta tgaccggaac tcgcgccgat aaccagaacc acggttatgc
     3301 ggttgggctg acctcaagct ggtttcagca cggtaagcag aagcaggggg cctggctgga
     3361 taactggttg cagtacgcgt ggtttagcaa tgatgtttct gaacatgaag atggcgtgga
     3421 tcattaccat tcgtcgggga ttatcgcctc gctggaagcg gggtatcagt ggttaccggg
     3481 gcgtggtgtg gtgattgaac cgcaggcgca ggtgatttat cagggcgtgc agcaggatga
     3541 ttttaccgcc gctaaccgtg cgcgcgtgtc acaatcgcag ggtgatgata ttcagacgcg
     3601 gctgggttta cacagcgaat ggcgtaccgc tgttcatgtc ataccaacat tagatctgaa
     3661 ttattatcac gatccccatt cgacggaaat tgaagaagat gccagcacta tcagtgacga
     3721 tgcggtgaag caacggggtg aaataaaagt gggagtcacg ggcaatatca gtcagcgagt
     3781 ttcgctgcgt ggtagcgtgg cgtggcagaa agggagtgat gattttgccc agacggcagg
     3841 gtttttgtcg atgacggtga aatggtaatc gctgtctgac catcactatt gctggatgcg
     3901 gcgtaaacgc ctaatcaggc ctacggagag catttgtagg cctgataaga cgcggcaagc
     3961 gtcgcatcag gcattggcgc ggttgccgga tgaatccaaa caacaacctc acttattctg
     4021 cgtgtgcagc atatagttca catccacgcc ggggccgagt ttaaaagtat tagtgatcgg
     4081 gttgtaatgt agcccggtta tatgtcgctc tttaaggctt gtctgatcta cccagcccag
     4141 cagttctgcc ggtttaataa acttcttcac atcatgtgta cctttaggca ccatgcgcaa
     4201 aatatattcc gcgccgacta ccgccatcag ccatgacttg ccgttgcggt taagcgtcga
     4261 gaaaaagaca tcgccgcccg gtttcaccag ttgcgcacag gctctgacca ctgactgcgg
     4321 atcagggacg tgctccagca tctccatgca ggtcaccaca tcatactgcc cggcatgttt
     4381 tgccgcgtgc tcttccacgg tttcctgcac gtaatccacc tgaatgccgc tttccagcgc
     4441 gtgcagcttt gccacctgca atggctcaaa gcccatatcc agaccggtca ccgtcgcgcc
     4501 ttcgcgcgcc atactctcgg ccagaatgcc gccgccacaa ccgacatcga gcaccttttt
     4561 gccaaataag ccgccagcac gctcggcaat atagctcaga cgcagcgggt taatgcggtg
     4621 cagcggtttg aactcacctt ccagatccca ccagcgggag gcgacggctt caaatttagc
     4681 gatctcttcg tggtctacgt tatggtttac cggcgatttt tcggcattca ttggcacttc
     4741 tactccgtaa ttggcaagac aaacgagtat atcaggcatt ggatgtgaat aaagcgtata
     4801 ggtttacctc aatctgcgcg gctgtgttat aatttgcgac ctttgaatcc gggatacagt
     4861 agagggatag cggttagatg agcgaccttg cgagagaaat tacaccggtc aacattgagg
     4921 aagagctgaa gagctcctat ctggattatg cgatgtcggt cattgttggc cgtgcgctgc
     4981 cagatgtccg agatggcctg aagccggtac accgtcgcgt actttacgcc atgaacgtac
     5041 taggcaatga ctggaacaaa gcctataaaa aatctgcccg tgtcgttggt gacgtaatcg
     5101 gtaaatacca tccccatggt gactcggcag tttatgacac gatcgtccgt atggcgcagc
     5161 cattctcgct gcgttacatg ctggtagacg gtcagggtaa cttcggttcc atcgacggcg
     5221 actctgcggc ggcaatgcgt tatacggaaa tccgtctggc gaaaattgcc catgaactga
     5281 tggccgatct cgaaaaagag acggtcgatt tcgttgataa ctatgacggc acggaaaaaa
     5341 ttcccgacgt catgccaacc aaaattccta acctgctggt gaacggttct tccggtatcg
     5401 ccgtaggtat ggcaaccaac atcccgccgc acaacctgac ggaagtcatc aacggttgtc
     5461 tggcgtatat cgatgatgaa gacatcagca ttgaagggct gatggaacac atcccggggc
     5521 cggacttccc gacggcggca atcattaacg gtcgtcgcgg tattgaagaa gcttaccgta
     5581 ccggtcgcgg caaggtatat atccgcgccc gtgctgaagt ggaagttgac gccaaaaccg
     5641 gacgtgaaac cattatcgtc cacgaaattc cgtatcaggt aaacaaagcg cgcctgatcg
     5701 agaagattgc ggaactggta aaagaaaaac gcgtggaagg catcagcgcg ctgcgtgacg
     5761 agtctgacaa agacggtatg cgcatcgtga ttgaagtgaa acgcgatgcg gtcggtgaag
     5821 ttgtgctcaa caacctctac tcccagaccc agttgcaggt ttctttcggt atcaacatgg
     5881 tggcattgca ccatggtcag ccaaagatca tgaacctgaa agacatcatc gcggcgtttg
     5941 ttcgtcaccg ccgtgaagtg gtgacccgtc gtactatttt cgaactgcgt aaagctcgcg
     6001 atcgtgctca tatccttgaa gcattagccg tggcgctggc gaacatcgac ccgatcatcg
     6061 aactgatccg tcatgcgccg acgcctgcag aagcgaaaac tgcgctggtg gctaatccgt
     6121 ggcagctggg caacgttgcc gcgatgctcg aacgtgctgg cgacgatgct gcgcgtccgg
     6181 aatggctgga gccagagttc ggcgtgcgtg atggtctgta ctacctgacc gaacagcaag
     6241 ctcaggcaat tctggatctg cgtttgcaga aactgaccgg ccttgagcac gaaaaactgc
     6301 tcgacgaata caaagagctg ctggatcaga tcgcggaact gctgcgtatt cttggtagcg
     6361 ccgatcgtct gatggaagtg atccgtgaag agctggagct ggttcgtgaa cagttcggtg
     6421 acaaacgtcg tactgaaatc accgccaaca gcgcagacat caacctggaa gatctgatca
     6481 cccaggaaga tgtggtcgtg acgctctctc accagggcta cgttaaatat cagccgcttt
     6541 ctgaatacga agcgcagcgt cgtggcggga aaggtaaatc tgccgcacgt attaaagaag
     6601 aagactttat cgaccgactg ctggtggcga acacgcacga ccatattctg tgcttctcca
     6661 gccgtggtcg cgtctattcg atgaaagttt accagttgcc ggaggccact cgtggcgcgc
     6721 gcggtcgtcc gatcgtcaac ctgctgccgc tggagcagga cgaacgtatc accgcgatcc
     6781 tgccggtgac cgagtttgaa gaaggcgtga aagtcttcat ggcgaccgct aacggtactg
     6841 tgaagaaaac cgtcctcacc gagttcaacc gtctgcgtac cgccggtaaa gtggcgatca
     6901 aactggttga aggcgatgag ctgatcggcg ttgacctgac cagcggcgaa gacgaagtaa
     6961 tgctgttctc cgccgaaggt aaagtggtgc gctttaaaga gtcttctgtc cgtgcgatgg
     7021 gctgcaacac caccggtgtt cgcggtattc gtttaggcga aggtgataaa gtcgtctctc
     7081 tgatcgtgcc tcgtggcgat ggcgcaatcc tcaccgcaac gcagaacggt tatggtaaac
     7141 gtaccgcagt ggcggaatac ccgactaagt cgcgtgcgac gaaaggggtt atctccatca
     7201 aggttaccga acgtaacggt ttagttgttg gcgcggtaca ggtagatgac tgcgaccaga
     7261 tcatgatgat caccgatgcc ggtacgctgg tacgtactcg cgtttcagaa atcagcatcg
     7321 tgggccgtaa cacccagggc gtgatcctca tccgtactgc ggaagatgaa aacgtagtgg
     7381 gtctgcaacg tgttgctgaa ccggttgacg aggaagatct ggataccatc gacggcagtg
     7441 ccgcggaagg ggacgatgaa atcgctccgg aagtggacgt tgacgacgag ccagaagaag
     7501 aataatttta cttcttcatg ccaaaaggga gccatctccc ttttttgaat tgaaaagtcc
     7561 aggctgcaaa gtctgggctt ttgtcgtatt agggcgcggt aaagtttggc tgtgtccgta
     7621 aaaaatggct ggctatacac aaggaatgtg gcaatgagtg gtgaaaaaaa gcgaaaggct
     7681 ggcggttcta tggtcttgta ggttttggcg caatagcact gctttccgct ggcgtctggg
     7741 cgttgcaata tgctggcagt gggccagaaa aaacgttgtc gccgctggtg gtgcacaata
     7801 atttgcaaat tgatctcaat gagccggacc tctttctcga cagcgattct ctaagccagc
     7861 ttcccaaaga tctcctcacc attccgtttc tccacgatgt tctgagtgaa gatttcgttt
     7921 tctattatca gaatcatgcc gatcgtctgg gcattgaagg cagcattcgt cgcattgtct
     7981 atgaacacga tctcacgctg aaagataagc tcttttcgtc actcttagat cagcccgcgc
     8041 aggcggcgct atggcacgat aaacaaggcc atctttcaca ttacatggtg ctgatccaac
     8101 gcagtggttt aagcaaactg ctggagccat tgttatttgc cgctaccagc gacagccagt
     8161 taagcaaaac ggaaatcagt agcatcaaga taaatagtga aactattcct gtttatcagt
     8221 tgcgctataa cggcaataac gccctgatgt tcgcgacata tcaggacaag atgctggtgt
     8281 tttccagcac ggatatgctg tttaaagatg atcagcagga taccgaagcc acggcgatcg
     8341 caagtgattt gttgagcggc aaaaaacgct ggcaagccag ctttggcctg gaagagcgtg
     8401 ctgccgaaaa aacgccagta cgccagcgca tcgtggccag cgccaggttg ctgggttttg
     8461 gctaccagcg gttaatgcct tcttttgctg gcgtacgctt cgaaatgggt aacgacggct
     8521 ggcacagttt tgtggcgtta aatgatgaat ccgccagcgt agatgccagt ttcgatttta
     8581 cgccggtctg gaacagtatg cctgccgggg ccagcttctg tgtggcggtg ccgtattcac
     8641 acggtattgc cgaagagatg ctttcgcaca tcagccagga aaacgacaag ttgaatgggg
     8701 cgttggacgg tgccgcgggg ctgtgctggt atgaagactc aaaattgcaa accccgctgt
     8761 ttgtcggtca gtttgatggc actgccgaac aggcgcaatt gccagggaaa ctgtttacgc
     8821 aaaatattgg tgcgcacgaa agcaaagcgc cagaaggtgt tttgccggta agccagactc
     8881 agcagggcga agcgcaaatc tggcgtcgcg aagtgagttc ccgatacggt cagtatccga
     8941 aagcgcaggc ggcgcaaccc gatcaattaa tgtcggatta ttttttccgc gtgtcgctgg
     9001 cgatgcaaaa caaaacgctg cttttctccc tcgatgacac gctggttaat aacgccctgc
     9061 aaacactgaa taaaacccgc ccggcaatgg tggatgtaat acccactgat ggcatcgttc
     9121 cgctctatat caatccacaa ggcgtggcga aactgctgcg taacgaaacg ttgaccagtc
     9181 tgccgaagaa tctcgaaccg gttttttata acgccgcaca aactttatta atgccgaagc
     9241 tggacgcttt atctcaacaa ccgcgttatg tcatgaagct ggcccagatg gaacccggtg
     9301 ccgcctggca gtggctaccc ataatctggc agccgctatg aggcacgggc tgctggcgct
     9361 gatttgctgg ctgtgttgtg ttgttgccca tagcgaaatg ctgaatgtcg aacaatccgg
     9421 gctgtttcgc gcctggtttg tacgcattgc acaagaacag ctccgccagg ggccaagtcc
     9481 acgctggtat cagcaggatt gtgcgggcct ggtgcgattt gcggcgaacg agacgctgaa
     9541 agttcacgac agtaaatggc taaaaagtaa cggtttatct agccagtatt tgccgccaga
     9601 gatgacgcta acacctgaac agcgtcaact ggcgcaaaac tggaatcagg ggaacgggaa
     9661 aaccggcccc tacgtgaccg cgattaattt gattcagtac aacagccagt ttattggcca
     9721 ggacataaac caggcgctgc ctggcgatat gatttttttc gatcagggcg atgcccagca
     9781 cttaatggtc tggatggggc gttacgtcat ctaccacacc ggaagcgcca cgaaaactga
     9841 caacggaatg cgcgcagtca gtctgcaaca acttatgaca tggaaggaca cccgatggat
     9901 acccaacgat tccaatccca atttcattgg catttatcgt ttaaattttc tggcgcgata
     9961 gcagcgtgct tgtccctgtc tcttgtggga acagggcttg ctaatgctga tgattcgctt
    10021 ccttccagta actatgcgcc gcccgccggg ggaacattct ttttgcttgc tgacagcagt
    10081 tttagcagca gtgaagagtc gaaagtgcga ctggaagcgc cggggcgtga ttatcggcgc
    10141 tatcagatgg aagagtacgg cggcgtggac gttcgcctgt atcgtattcc tgacccgatg
    10201 gcatttttgc gccagcagaa aaacctgcat cgcattgtgg tgcaaccgca atatctgggc
    10261 gacgggctga acaatacgct gacctggctg tgggataact ggtacggcaa atctcgccgc
    10321 gtgatgcagc gtactttctc ttctcagtca cggcagaatg tgactcaggc attacccgaa
    10381 ttacagctcg gcaatgccat tattaaacct tcccgttatg tacagaacaa ccagttttcc
    10441 ccgctgaaaa aatatcccct ggtggaacag ttccgttatc cactatggca ggctaaaccg
    10501 gtcgagccgc agcaaggggt aaaactggaa ggcgcatcca gcaatttcat ctcgccgcag
    10561 ccgggtaaca tttatattcc tctcggccaa caagagccgg gactgtacct cgtcgaggcg
    10621 atggttggtg ggtatcgggc gacgacggtg gtgtttgttt ccgataccgt ggcgcttagc
    10681 aaagtgtcag gcaaagagct tctggtgtgg accgcgggta aaaaacaggg tgaagcgaag
    10741 cccggctcag agatcttgtg gactgacggt cttggcgtga tgacccgcgg tgtgaccgat
    10801 gacagcggta ccttgcagtt acaacatata tcgccagaac gttcatacat tctgggtaag
    10861 gatgctgaag gcggcgtttt tgtctccgag aacttcttct acgaaagcga aatctacaac
    10921 acccgcttgt atatttttac cgatcgcccg ctatatcgcg caggcgatcg tgtcgatgtt
    10981 aaagtgatgg gccgcgagtt ccacgatccg ttgcattcat cccccatcgt cagcgccccg
    11041 gcgaagcttt cggtgctgga cgctaacggc agtctgttgc aaaccgtcga tgtcacgctg
    11101 gatgcgcgca atggcgggca gggaagtttc cgcctgccag aaaatgccgt tgccggaggt
    11161 tatgagttac gtcttgctta ccgcaatcag gtctatagca gcagttttcg cgtggcaaac
    11221 tacatcaagc cacatttcga gattggttta gctctcgaca aaaaagagtt caaaactggc
    11281 gaagcggtca gcggcaaact gcaacttctc tacccggatg gcgagccggt aaaaaatgcc
    11341 cgcgtgcagt taagtttgcg cgctcagcaa ttatcaatgg tcggtaacga tttgcgttat
    11401 gccggacgtt tccccgtgtc gctggaaggc agcgaaacgg tgtccgacgc cagcggtcat
    11461 gtggcgttaa atctccccgc cgccgataaa ccgagccgct atttgttaac cgtctccgcc
    11521 agtgacggcg cggcgtatcg cgtcaccacc accaaagaga tcctcattga acgcggcctg
    11581 gcgcattact ctttaagtac cgccgcacaa tacagtaata gcggcgagtc ggttgtgttc
    11641 cgttatgccg cgctggaatc ttcaaaacag gttcctgtta cgtatgaatg gttgcgtctc
    11701 gaagaccgca cgagccatag cggagatcta ccgtcaggcg gcaaatcctt taccgtcaat
    11761 ttcgataaac ctggcaacta caatctgacg ttacgcgata aagacggctt aattctcgcc
    11821 gggttaagcc atgccgtcag cggtaagggc agtatgtcgc atactggtac ggtagatatc
    11881 gtggcagata aaacgctgta ccagcctggc gaaaccgcga agatgctgat taccttcccg
    11941 gagccaattg atgaagcatt attgacgctg gaacgcgatc gcgttgaaca gcagtcgctg
    12001 ctatcgcatc cggcaaactg gctgacgtta caacgtttaa acgatactca atatgaagcc
    12061 cgtgttccag tgagcaattc ctttgcgcct aacatcactt tttcggtgct gtatacccgt
    12121 aacggtcagt acagttttca gaacgccggg atcaaagttg ccgttcccca gctggatatc
    12181 cgggtgaaaa cggacaaaac ccattaccag cctggtgaac tggtcaatgt cgaattaacc
    12241 tcgtcgctga aaggtaaacc tgtttctgcg cagctaacgg taggcgtggt cgatgaaatg
    12301 atctacgcgc tgcaaccaga aatcgcgccg aatatcggca aatttttcta tccgctgggg
    12361 cgtaacaatg tgcgtaccag ctccagtctg tcgtttatca gctacgacca ggcactctcc
    12421 agcgagccgg ttgcgcctgg cgcaactaac cgcagcgagc ggcgagtaaa aatgcttgaa
    12481 cgtccacggc gtgaagaggt ggataccgcg gcatggatgc cgtcactcac aaccgataaa
    12541 caaggcaaag cgtatttcac gttcctgatg cctgattcgt taacccgctg gcgtatcacc
    12601 gcgcgtggga tgaacggcga cgggctggtc gggcaggggc gtgcttatct gcgttcggaa
    12661 aaaaatctct acatgaagtg gagtatgcca acggtgtatc gcgtgggcga caaaccgtcg
    12721 gcaggactgt ttatcttcag tcagcaggat aacgaaccgg tggcgctggt gactaaattt
    12781 gcaggcgctg agatgcgcca gacgctgacg ctgcacaaag gggcgaatta tatttcgctg
    12841 gcgcagaaca ttcagcaatc tggcttgtta agcgcagaac tgcaacaaaa tgggcaagtg
    12901 caggacagca ttagcacaaa actgtctttt gtggataaca gctggcccgt tgaacagcag
    12961 aaaaatgtca tgctcggcgg tggcgataac gcgctgatgt tgcccgagca ggcgagcaat
    13021 atccggctac aaagtagtga aacgccgcag gagattttcc gcaacaatct tgatgcgtta
    13081 gtcgatgaac cgtggggggg ggtgatcaac accggtagcc gtctgatccc gctcagtctc
    13141 gcctggcgtt cgcttgccga tcatcaaagt gccgccgcta acgacattcg tcagatgatt
    13201 caggataacc gtctgcggct gatgcaactg gcggggcccg gagcgcgctt tacctggtgg
    13261 ggtgaagatg gcaatggtga cgccttcctt acggcatggg catggtacgc cgactggcag
    13321 gccagccagg cgctcggcgt aacgcaacaa ccggaatact ggcagcatat gctcgacagt
    13381 tatgccgagc aggcagataa catgccgtta ttgcatcggg cgctggtgct ggcgtgggca
    13441 caggagatga atctgccgtg caaaacgttg ttgaaagggt tggatgaagc tatcgcccgg
    13501 cgcggaacta aaactgaaga tttctctgag gaagacaccc gcgatatcaa tgacagcctg
    13561 atcctcgata caccggaatc tccactggca gatgcggtgg caaacgtctt aaccatgacg
    13621 ttgctgaaaa aagcgcagtt gaagtccacg gtgatgccac aggttcagca atatgcgtgg
    13681 gataaagcgg taaacagcaa tcagccgctg gcacacacgg ttgtgctgct caatagcggg
    13741 ggcgacgcta cccaggcggc tgctatttta agtggtttga ccgctgagca atctactatt
    13801 gagcgcgcgc tggccatgaa ctggctggcg aaatatatgg cgacaatgcc ttcggttgtg
    13861 ttgcctgcgc ctgcgggcgc atgggccaaa cataagttaa ctggaggggg cgaatactgg
    13921 cgttgggttg gtcagggcgt gccggacatt ctctcttttg gtgacgaatt atcgccgcaa
    13981 aatgtgcagg tccgctggcg tgaagcggca aaaacggctc aacaaagtaa cattccggtg
    14041 accgttgaac gccaattgta tcggcttatc cctggtgaag aagagatgag ctttactctg
    14101 caaccggtga ccagcaatga gattgacagc gatgcgctgt atctcgatga aattacgctt
    14161 accagcgagc aggatgcagt tctgcgctat ggtcaggtgg aagtaccgct cccgccggga
    14221 gccgacgttg agcgcacaac atggggcatt tcagtcaata aacccaacgc tggaaaacag
    14281 caggggcaat tgctggaaaa agcgcgaaat gaaatgggcg aactggccta tatggtgccg
    14341 gtgaaagaac tgacgggaac ggtcactttc cgccatttgc tgcgcttctc gcaaaaaggg
    14401 caattcgttc tgcctcctgc tcgttatgtg cgttcctatg cacctgcaca gcaaagtgtt
    14461 gcggcaggga gcgaatggac cgggatgcag gtgaaataag tgaactggcg cagaatcgtc
    14521 tggctattgg cgttggttac tctgccaacg cttgcggagg agacgccgtt acaactggcg
    14581 ctgcgcggtg cgcagcacga tcaactgtat cagctgtcgt catcaggcgt gaccaaagtc
    14641 tcagcattac ctgacacgct caccacgccg ttagggagtt tatggaaact gtatgtttac
    14701 gcctggctgg aggataccca tcaaccggag cagccctacc agtgtcgcgg aaactccccg
    14761 gaagaggttt attgttgtca ggctggggag agcataacgc gcgataccgc gctggtacgt
    14821 tcctgtgggc tctattttgc gccgcagcgg ttgcatattg gggcagatgt gtgggggcaa
    14881 tactggcaac agcgtcaggc acctgcgtgg ttagcgtctc tttctacgct gaagccagaa
    14941 acctcggtaa cggttaaatc gctgcttgat tcattagcga cgttacctgc gcaaaacaaa
    15001 gctcaggaag tcttactgga tgtggtgctg gacgaggcga aaatcggtgt cgcatctatg
    15061 ctcggtagtc gggtacgggt gaaaacctgg agctggtttg ccgatgataa acaggaaatc
    15121 cgtcagggtg gttttgctgg ctggttaacc gatggtacac cgctgtgggt gactggcagt
    15181 ggcaccagca aaacggtact gacccgttat gcgacggtgc ttaatcgggt tttgccagtg
    15241 ccgacgcaag ttgccagtgg gcaatgtgta gaggtggagc tatttgcgcg ctagccgctg
    15301 aagaaaatca cagcggagaa gagtacgacg gcagtaaaac ccggcgtact taatgggcgc
    15361 tatcgtgtga cgtttgccaa tggtaaccac attacgtttg tcagccacgg tgagacgacc
    15421 ttactgaccg aaaagggcaa actgaaattg cagtcgcatc tcgatcgtga agagtacgtt
    15481 gccagagttc tggatcgcga ggcgaaaagt acgccacctg aggcagcgaa agccatgact
    15541 gtggcgatac gcactttttt acagcaaaac gccaatcggg aaggcgattg cctgaccatc
    15601 cccgacagca gcgccacgca gcgtgtttct gcttcgccag ctaccacggg ggcgcgaacg
    15661 atgaccgtct ggacgcagga tctcatctat gcgggcgatc cggttcatta tcacggcagt
    15721 cgcgctaccg aagggacgct ttcctggcgg caagccatgg cccaggcggg gaagggagag
    15781 cgttacgacc agatactcgc gtttgcttat cccgacaaca gccttagccg ctggggagcg
    15841 ccacgcacaa cctgtcagtt attacccaaa gcgaaagcct ggctggcgaa aaaaatgccg
    15901 cagtggcggc gtatattaca aggcgagacg gggtacaacg aaccagacgt gtttgcggtc
    15961 tgtcgtttag tctcaggttt cccctatacc gatcggcagc agaaacggct gtttattcgt
    16021 aacttcttta cgcttcagga tcggctggat ttaacccatg agtatctgca cctggccttt
    16081 gatggttatc ccaccgggct tgatgagaac tatatcgaaa cgctgacccg acaattatta
    16141 atggactaat gctatgcgaa agatttttct tccgttgtta ctggtggcgc tttcgcctgt
    16201 cgctcacagt gaaggtgtgc aggaagtcga gattgacgca ccgctctctg gctggcatcc
    16261 ggcagagggc gaagatgcca gtttttcaca atcgatcaat tacccggcgt cgtccgtcaa
    16321 catggcagat gatcaaaata tctcagcgca gatccgcggc aaaataaaga attatgctgc
    16381 ggcgggtaaa gttcagcagg gccggctggt ggtcaacggt gccagtatgc cgcagagaat
    16441 tgaatccgat ggttcatttg cacgccctta tattttcact gaaggcagca acagcgtgca
    16501 ggtcatcagc ccggatgggc aaagccgaca aaaaatgcag ttttactcaa cgccgggtac
    16561 aggaacgatt cgtgcacgtt tacggctggt tctctcgtgg gatacggaca ataccgacct
    16621 cgatcttcac gttgtcacgc ccgatggcga acacgcctgg tacggtaaca ccgtgctgaa
    16681 aaacagtggt gcattggata tggatgtcac gacggggtac ggacccgaga ttttcgccat
    16741 gccagcacca gttcacgggc gctatcaggt gtatatcaac tactatggtg ggcgcagcga
    16801 aacggaatta accaccgccc aactgacgct gatcaccgat gaaggatcgg tcaatgagaa
    16861 acaggaaaca tttattgtac cgatgcgtaa tgctggcgag ctgacgctgg tgaaaagttt
    16921 tgactggtga tccatccctt cacacaacct ggctctttct tttttcagat aagactgctg
    16981 ggcgatctgc cgacgccagg atataaccat taacaagcgt cggcagattg gtcatttctt
    17041 atttttcgta ctgagaactg tccatttact ccggctttcc tggttaaggc aatggtttct
    17101 aagcgatacg cattattgca atggtgacag aacattgcgg taacacgctt ttaccgctac
    17161 cttaaccaca ctccatcggt cacctgaggc ggagcttcgc ccctttgaaa taccttgctt
    17221 cttttcgtac aaccctgaaa gcctcgcgct acatgttcag agcattggcg ttagtgctct
    17281 ggctgttgat tgctttttca tccgtttttt acatcgttaa tgcgttacat cagcgagaat
    17341 cggaaattcg tcaggaattt aatctgagtt ccgatcaggc tcagcgcttt attcaacgca
    17401 cctctgatgt gatgaaagag ctgaagtaca tcgccgaaaa tcgcttatcg gcagaaaacg
    17461 gtgtgctttc cccgcgtgga cgagaaacgc aggcggatgt gcctgcgttt gaaccgctgt
    17521 ttgccgactc cgattgttca gcaatgagta acacctggcg aggttctctg gagtcattgg
    17581 cgtggtttat gcgctactgg cgcgataatt tttctgcggc ttacgatctc aaccgggtat
    17641 ttttaatcgg cagcgataac ctctgcatgg ccaatttcgg tctgcgtgat atgccagtgg
    17701 aacgcgatac cgcgttgaaa gctttgcatg aacgcatcaa taaatatcga aatgcaccac
    17761 aagatgatag cggcagtaac ctctactgga tcagcgaagg tccgcgccct ggcgtcgggt
    17821 atttttacgc gttgacgcca gtttatctgg cgaaccggtt gcaggcgctt ttgggtgtcg
    17881 agcagaccat ccggatggag aactttttct taccgggtac gttgccgatg ggggttacca
    17941 ttcttgatga aaatggtcat accctgattt cgcttaccgg accagaaagc aaaattaagg
    18001 ccgatcctcg ctggatgcag gaacgctcct ggtttggcta tacggaaggg ttccgggagc
    18061 tggtgctgaa gaaaaatctg ccaccctcat cgctcagcat cgtgtattcg gtgccggttg
    18121 ataaggtgct ggaacgcatt cgcatgttga tccttaacgc aattttgctg aatgtgcttg
    18181 ccggagctgc attgtttact ctcgcgcgga tgtacgagcg acgtattttc attccggcgg
    18241 aaagcgacgc cctgcgactg gaagaacatg agcagttcaa tcgcaagatt gtcgcctccg
    18301 cgccagtggg tatctgcatt ttgcgtaccg ctgatggcgt caatatttta agtaacgaac
    18361 tggcgcatac ctatctcaat atgcttacgc atgaggaccg ccaacgactg acacaaatta
    18421 tctgtgggca gcaggtcaat tttgttgatg tcctgaccag caacaatacc aatctgcaaa
    18481 tcagcttcgt ccattcgcgc tatcgtaatg aaaacgtggc catttgtgtg ctggtggatg
    18541 tttcttcgcg cgtgaagatg gaagagtcgt tgcaggagat ggcacaagca gcggaacagg
    18601 cgagccagtc aaaatcgatg ttccttgcca ccgtcagtca tgagctgcga acgccgctgt
    18661 atggcattat cggtaacctg gatctgttgc aaaccaaaga gttaccgaaa ggcgtcgatc
    18721 gtctggtgac ggcaatgaac aactcttcca gcctgttgtt gaaaattatc agcgatattc
    18781 tcgatttctc gaagattgaa tcggaacagt tgatgatcga accgcgtgag ttttcaccgc
    18841 gtgaagtgat gaaccacatc accgccaact atttaccgct ggtggtacgc aagcagttag
    18901 gcttgtactg ctttattgaa ccggatgtgc cagtggcctt aaatggcgac ccgatgcgtt
    18961 tacagcaggt catctccaac ctgttgagta acgccataaa attcaccgat accggctgta
    19021 tagttttgca tgtccgcgct gatggcgatt atctctctat ccgtgttcgc gataccggcg
    19081 tggggattcc ggcgaaagaa gtggtgcgct tgtttgatcc cttcttccag gtcggaacgg
    19141 gcgtacagcg aaacttccag gggaccggtc tgggtctggc gatttgtgaa aaactgatca
    19201 gcatgatgga cggcgatatc tcggtagatt cagaaccggg aatgggcagc cagtttaccg
    19261 tgcgtattcc gttgtacggc gctcagtacc cgcagaaaaa aggcgtggaa gggttgagtg
    19321 gtaaacgctg ctggctggcg gtccgcaatg cgtcgctctg tcagttcctg gaaaccagtt
    19381 tgcagcgcag cggcatcgtc gtaacaacat acgaagggca ggaaccgact cccgaagatg
    19441 tgttaatcac tgacgaggta gtgagtaaaa aatggcaggg cagagcggta gtgaccttct
    19501 gtcgtcgaca tattggtatt ccgctggaga aagcgccagg ggagtgggta cacagtgtgg
    19561 ctgctccgca tgagctaccg gcattgttgg cgcgtattta tttgatcgag atggagagcg
    19621 acgatcctgc taacgctctg ccgtcgacgg acaaagcggt cagcgataat gacgatatga
    19681 tgattctggt cgtggatgat catccgatta accggcgttt gctggcagat cagttgggat
    19741 cgttgggcta tcaatgtaaa accgcgaatg atggcgtcga tgcgcttaat gtacttagca
    19801 agaatcatat tgatatcgtg cttagcgacg tcaacatgcc aaatatggat ggttaccgct
    19861 tgacacaacg cattcgtcag ttgggactga cgttgccggt aatcggagta acggctaatg
    19921 cgttggctga agagaagcag cggtgtctgg agtccggtat ggacagctgc ctgtcgaagc
    19981 cagtaacgct ggatgttata aaacagacgc tgacggtata tgccgagagg gtcaggaaat
    20041 cgcgggaatc gtaggacgga taaggcgttt acgccgcatc tggcattcag tgcaaatgcc
    20101 agatgcgacg ctgacgcgtc ttatctggcc tactttaatg taatctgttg aaataatggg
    20161 aatcgtagga cggataaggc gtttacgccg catccggcaa tcggtgcaaa tgccagataa
    20221 gacactaacg cgtcttatct ggcctacaga tgattagtct ttatctgccg gacttaacgt
    20281 tactgaagag agataattca gcagggcgat atcgttctcg acacccagct tcatcatcgc
    20341 agatttcttc tggctactga tggttttaat actgcggttc agctttttag cgatctcggt
    20401 caccaggaag ccttccgcaa acaggcgcag aacttcactc tcttttggcg agagacgttt
    20461 gtcaccgtaa cctccagcac tgattttttc caacaggcga gaaacgcttt ccggggtaaa
    20521 tttctttcct ttttgcagcg ctgcgagggc tttcggcaga tcggtcggtg caccttgttt
    20581 cagcacgatc ccttcgatat ccagatccaa taccgcacta agaatcgccg ggttgttgtt
    20641 catggtcaga acaataatcg acaggcttgg gaaatggcgc ttgatgtact taattaaggt
    20701 aatgccatcg ccgtacttat cgccaggcat ggagagatcg gtaatcaaca catgcgcatc
    20761 cagtttcggc aggttgttga tcagtgctgt agagtcttca aattcgccga caacattcac
    20821 ccactcaatt tgctcaagtg atttgcgaat accgaacaag actatcggat ggtcatcggc
    20881 aataattacg ttcatattgt tcatgtaata ggctaccttg ctacagcaag ctcttgacat
    20941 aactgtcaat gtcgctgatg tatttttcta ttcctggaac atccttctca cgaatcagat
    21001 gttccagcgt ttcacataac tgcttgccgg gtaccagatt tagcatggca aatacgcctt
    21061 taagacgatg agccgtttgg gctaacgcag caaagtcact ggttgctgct tcagtataca
    21121 gcctcttaac atcatccggt actgtgtcta caaagagcgc ataatagccg ctggcatgga
    21181 gttgcgcatt ttcatctccg cccagaggcg attctgtcac ctcttcctgc gccagttgca
    21241 cttcaattaa ttgtaagacc gcttcctgca tagcgttgct catattgaag ttgacgcaca
    21301 attgaccagg cccaatttcc cgtacgccag actcatcatc gcttaaaagc aagccagagg
    21361 cagtaagatt agacggatta tccgttaaaa agatatcata atcttgacta attaatcttt
    21421 catcgggtgt gatacaggtt gcaccccaat tttctaactg gcgagtgaca atattccgaa
    21481 tttctgccga agtaacatcc accattacgc agacatcatc cagtaaacgc tcttcttcct
    21541 cttcaacttc cgggtcagct gcgagcattt tgatatgcac agagtagcgt gtaccaagcc
    21601 catcccgcgt tttgatgttt aaatgaccgc ccagtttacg tgccagttga tcgctcagcc
    21661 agaatgccag cgggtccgcc ttgccatagc gatcgttttg ggtctggttg ataaacggga
    21721 agtgcaaatt atccatttca tgaatgctta cgccttctcc ggtgtccaga atgcggaacg
    21781 tcaggcggtc ttcggaggac tcatcctgat caacctcaag ggtgattttt cccaattgcg
    21841 ttgaggtcac ggcatattgc atcagtagca gcaaaatacg tcgtaatgca tcgcgatcgc
    21901 cgcggcgcat atcgtgtgct ttcagatgat tgttaatcag cagttgcaga cctttacgct
    21961 tgatggcagg caacactgaa ggcacaactt catcaattaa atcctgcacg gagaacagca
    22021 ccgtctcact tttccagcta tcgtccgcaa gcatgttcgc taactgtatt tcatcgacca
    22081 atcgcaccag cacatctgcc tgattcgcca gttgtttgct ttccggggcg ttgagtttag
    22141 ccgcgctctc cgccagggac tgtgcgggtt ctttcagcgc atcgccaatg tttttcataa
    22201 aggtcatccg cccctgctgg tttttctcat acagacgctg cgcctgcttg agtttcttgt
    22261 ttaccagcac ttcgcgatcc tgatcgcgaa taatgaaaat ttgtgtgcgc ggcgcaacct
    22321 ggctgcggaa catgcggatc tcatacagct cgttattgat cgtcgcctga ataatcccct
    22381 gatgctgttc cgccatggtg gtgatgtttt gcagattcaa atgcggcagc aaatgatcgg
    22441 caattttgtt acttatgaca gtgcggttcg attcctgatc gtgaaccagc aggccgagcg
    22501 gcagcagtga gactatctct tcattgattg cccgtaaaat gcgcaattcg ttattgaccg
    22561 ccgtgttggg tagactttct gtactgcgac tggagaaatg gcggaatgtg gtatagccaa
    22621 ataacgccag cgccagcaaa ccgatgttca gcagcagtgg cagcagaatg ttttgcaacg
    22681 tatccagcaa taaggtgcca taaggaacct gccagaccag gcgcatatcg gtagagttga
    22741 gtgccgagga gatttcaatc ttcgtactgt taaagtggat actgacgcta tccgttcctt
    22801 ctttctcatt atcattgttt cccgttgccg tcgcgtctgg ctcaaggcgg aaactgtcca
    22861 gcggcatacc cggtgggatc aaatcattaa tcggcagatc aaaagccacg accgttgcca
    22921 gatgtcccgg ctggttgaac gtggtacgca aggtaaagta atgaccgttc tgccaggcca
    22981 ggcggcgcat gttagaaaaa ctttcgcgtt catcgagggc gttggcctgt tgcaacatct
    23041 ctgcacgacg tgaatcaaca atgtcactga cggtcgattc tttaaatccg gaggtgagat
    23101 ctttgagggg cagggttgag atcagcacca gactgttatc ctgaccattc aggtaataca
    23161 ttgaccacgg tacattttct gcgccccaca atgtgtccag ataggtggac atccgttgag
    23221 tcatctcaag cgttgagttg tcgtgagagc caaagattaa cgcttccgtt ttgcggcgcg
    23281 gtttttccag atagtagaca tcctgtttca ggcgcgtctc ttgtaaacct tcgccggagg
    23341 agggggaggt cgtcgcggca atgttgtcgt agatctgcca ggtcacgtaa cgccaggtat
    23401 cgacgcgctt ttggatagcg tgggtaatgt cgacaatttg gtaactttta tctttcagcc
    23461 aggcgttaac ggcgctttgt accattacac ccatcgtcac cagtaacaca atgatcaaca
    23521 gtaaaaagaa gcgggtaatg ctccccggta ggagtgaaaa gcgggtcgtg gccgttgtct
    23581 ctttctgacg cattcgtgtt tatgacctgt taaaacttcg cgaagtgata gggcagtata
    23641 aagggtacag tgtgaaattc cagactctta cctgattgat gattctctat tctcccgtga
    23701 tatttatcat cctgcattgg gggaaataga tgtacaagcg ccattttgct gtacaaataa
    23761 cctacaaaaa gcccaacgaa aataattgca aataaacaaa gattgctgga aattatgctg
    23821 atgttaatta tttgtgaaat agttaacaag cgttatagtt tttctgtggt agcacagaat
    23881 aatgaaaagt gtgtaaagaa gggtaaaaaa aaccggatgc gaggcatccg gttgaaatag
    23941 gggtaaacag acattcagaa atgaatgacg gtaataaata aagttaatga tgatagcggt
    24001 agttattcta gttgcgagtg aaggttttgt tttgacattc agtgctgtc
//
LOCUS       DAZKIP010000065        23804 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZKIP010000065 DAZKIP010000000
VERSION     DAZKIP010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23804
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.065"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(76..1341)
                     /locus_tag="ACHOUK_002814"
     CDS             complement(76..1341)
                     /locus_tag="ACHOUK_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9898225.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALVVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQDVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANRERFISPFEYAKINNPLKQ"
     gene            complement(1539..1614)
                     /locus_tag="ACHOUK_002815"
     tRNA            complement(1539..1614)
                     /locus_tag="ACHOUK_002815"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1579..1581),aa:Phe,seq:gaa)
     gene            complement(1720..2427)
                     /gene="yqgA"
                     /locus_tag="ACHOUK_002816"
     CDS             complement(1720..2427)
                     /gene="yqgA"
                     /locus_tag="ACHOUK_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFP9898226.1"
                     /translation="MVIGPFINASAILLGGMLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            2580..2663
                     /locus_tag="ACHOUK_002817"
     CDS             2580..2663
                     /locus_tag="ACHOUK_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9898227.1"
                     /translation="MHAISLHSLAYRRFARNPSLFMLLLMR"
     gene            2825..4960
                     /gene="speC"
                     /locus_tag="ACHOUK_002818"
     CDS             2825..4960
                     /gene="speC"
                     /locus_tag="ACHOUK_002818"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFP9898228.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILTLLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENIFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGIDAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGDVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(5010..6266)
                     /gene="nupG"
                     /locus_tag="ACHOUK_002819"
     CDS             complement(5010..6266)
                     /gene="nupG"
                     /locus_tag="ACHOUK_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFP9898229.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6468..7547)
                     /gene="mltC"
                     /locus_tag="ACHOUK_002820"
     CDS             complement(6468..7547)
                     /gene="mltC"
                     /locus_tag="ACHOUK_002820"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFP9898230.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGGTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7612..7887)
                     /gene="yggX"
                     /locus_tag="ACHOUK_002821"
     CDS             complement(7612..7887)
                     /gene="yggX"
                     /locus_tag="ACHOUK_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFP9898231.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7915..8967)
                     /gene="mutY"
                     /locus_tag="ACHOUK_002822"
     CDS             complement(7915..8967)
                     /gene="mutY"
                     /locus_tag="ACHOUK_002822"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFP9898232.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            9128..9847
                     /gene="trmB"
                     /locus_tag="ACHOUK_002823"
     CDS             9128..9847
                     /gene="trmB"
                     /locus_tag="ACHOUK_002823"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFP9898233.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMIEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            9847..10173
                     /gene="yggL"
                     /locus_tag="ACHOUK_002824"
     CDS             9847..10173
                     /gene="yggL"
                     /locus_tag="ACHOUK_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFP9898234.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERNLDEVRTSEL
                     FDVWWD"
     gene            10357..11076
                     /gene="yggN"
                     /locus_tag="ACHOUK_002825"
     CDS             10357..11076
                     /gene="yggN"
                     /locus_tag="ACHOUK_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFP9898235.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            11252..12298
                     /gene="ansB"
                     /locus_tag="ACHOUK_002826"
     CDS             11252..12298
                     /gene="ansB"
                     /locus_tag="ACHOUK_002826"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFP9898236.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDDVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFIASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12415..13422
                     /gene="yggM"
                     /locus_tag="ACHOUK_002827"
     CDS             12415..13422
                     /gene="yggM"
                     /locus_tag="ACHOUK_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFP9898237.1"
                     /translation="MKKQWIVGTALLMLMTGNVWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWTVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLETALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREDAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWITLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(13574..14710)
                     /gene="hemW"
                     /locus_tag="ACHOUK_002828"
     CDS             complement(13574..14710)
                     /gene="hemW"
                     /locus_tag="ACHOUK_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFP9898238.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVKTIFIGGGTPSLLSGSAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEPAPRVEFSAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(14703..15296)
                     /gene="rdgB"
                     /locus_tag="ACHOUK_002829"
     CDS             complement(14703..15296)
                     /gene="rdgB"
                     /locus_tag="ACHOUK_002829"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFP9898239.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTELGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(15304..15594)
                     /gene="yggU"
                     /locus_tag="ACHOUK_002830"
     CDS             complement(15304..15594)
                     /gene="yggU"
                     /locus_tag="ACHOUK_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFP9898240.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEVAALIN"
     gene            complement(15591..16157)
                     /gene="yggT"
                     /locus_tag="ACHOUK_002831"
     CDS             complement(15591..16157)
                     /gene="yggT"
                     /locus_tag="ACHOUK_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFP9898241.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(16175..16879)
                     /gene="yggS"
                     /locus_tag="ACHOUK_002832"
     CDS             complement(16175..16879)
                     /gene="yggS"
                     /locus_tag="ACHOUK_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFP9898242.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            16897..17877
                     /gene="yggR"
                     /locus_tag="ACHOUK_002833"
     CDS             16897..17877
                     /gene="yggR"
                     /locus_tag="ACHOUK_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFP9898243.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLTENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGTPPILPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVGEGRL"
     gene            complement(18068..18484)
                     /gene="ruvX"
                     /locus_tag="ACHOUK_002834"
     CDS             complement(18068..18484)
                     /gene="ruvX"
                     /locus_tag="ACHOUK_002834"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFP9898244.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKIDSASAVIILESYFEQGY"
     gene            complement(18484..19047)
                     /gene="yqgE"
                     /locus_tag="ACHOUK_002835"
     CDS             complement(18484..19047)
                     /gene="yqgE"
                     /locus_tag="ACHOUK_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFP9898245.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(19159..20106)
                     /gene="gshB"
                     /locus_tag="ACHOUK_002836"
     CDS             complement(19159..20106)
                     /gene="gshB"
                     /locus_tag="ACHOUK_002836"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFP9898246.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQ"
     gene            complement(20119..20850)
                     /gene="rsmE"
                     /locus_tag="ACHOUK_002837"
     CDS             complement(20119..20850)
                     /gene="rsmE"
                     /locus_tag="ACHOUK_002837"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFP9898247.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(20930..21637)
                     /gene="endA"
                     /locus_tag="ACHOUK_002838"
     CDS             complement(20930..21637)
                     /gene="endA"
                     /locus_tag="ACHOUK_002838"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFP9898248.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDHYNLTLSRQQTQLFNAWDKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(21732..22229)
                     /gene="yggI"
                     /locus_tag="ACHOUK_002839"
     CDS             complement(21732..22229)
                     /gene="yggI"
                     /locus_tag="ACHOUK_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFP9898249.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYSCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(22306..23700)
                     /gene="galP"
                     /locus_tag="ACHOUK_002840"
     CDS             complement(22306..23700)
                     /gene="galP"
                     /locus_tag="ACHOUK_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFP9898250.1"
                     /translation="MPDAKKQGQSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAGLNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
BASE COUNT         5864 a         5985 c         6239 g         5716 t
ORIGIN      
        1 ctaataccgt tttgtctgtt tttttaacaa agaaaataat cctggagtag gttcccgggc
       61 atttgcccgg gatgattact gttttaatgg attattaatc tttgcatatt caaatggact
      121 gataaatctt tctctgtttg catccagaaa atcggcccac cactgtagca tcagtcgccg
      181 ttcttccaga tgttctgctt tatgaatgta agcagcacgt acaccgttac gttcctgatg
      241 gctcatctga cgttcaactg catcgcggga ccacaaacct gattctatta acgcactaca
      301 tgccatcgcc cggaatccat gaccacagac atcctgggtt gtgtcatacc ccataaccct
      361 cagggcttta tttacggtgt tttcactgat tggtttacgt gggtcatgtg ctcccgtaaa
      421 gatcagacca ttttcaccag cccatgtctg aagttctgcc agtatggcta caacctgttt
      481 gctgagtggc acaagatgtg gtgttcgcat cttggatccc cggtgtgaga attttacgcc
      541 tggcagaggt tcgcgttcgg ctggaatagt ccacatggca ttgtcaatat caatttcaga
      601 ccagcgggcg tatctgagtt cactggaacg aataaaaacc agtaaattca gcataacggc
      661 aagctgggtg acaggttgcc ccttgtaact gccaatacgc gacaaaagtt caggcaggcg
      721 agaaagatca agagcggggc gatgctggcg ttttaccgta gtcaatgcgc cagccatatc
      781 cagagcagga ttatatctga tgatgccaga ctgtacggca taacgcatta cggcagatat
      841 tctttgctgc agacgactgg cgatttcata aatttgttta gcttctgcag cacgaacagg
      901 gataagcagg tccggggtat tgagtgttgt gatatcccga ttgccgataa aggggaatac
      961 gtgtgtctcg aggcttttga gtactttatc gctgtgtgat tgtgcccatt gtttattact
     1021 ggcatgccag cgtcgtgcaa tatgttcgaa agagagttgt aaatcaggga ctactttttt
     1081 agctcttttc tgttcgcaag ggtcaacccc ctccgaaatc agttttcggg cctcatcccg
     1141 ttttgtacga gcttcggaga gagatacttc tggatacaca acaagtgcca gagtcttctc
     1201 tttacccaga atacgataac ggagacgcca gtaacgggaa ccattaggat gtaccagcag
     1261 gaacatgcca gccccgtcag tgagtttata agccttgtca gtgggctttg cagcccggat
     1321 ttttgcgtca gtcagtgcca tagttcagcc ttctgaatgc gttaaagagg gtacaaattc
     1381 tgtcgaactg gggtatgccc gcaattatac ccacacaatg aggttgatgt ggggagaatt
     1441 tggttgagtt cggtagaatt gatttggagg aagaacactt aaatcatggt gttctgaaac
     1501 gaaaaacgga cctccgtgga ggtccgttga tatgaatttg gtgcccggac tcggaatcga
     1561 accaaggaca cggggatttt caatcccctg ctctaccgac tgagctatcc gggcaacggg
     1621 gcgcattaaa cctgattcgc ctcgtctcgt caatcaaatt tcttcaattt actgcagact
     1681 gcacaatcta tcatcacttt gccgctattg cacacattgt caggcaaacc aggcggtcca
     1741 ggcggcggaa agcggcattg ccagaaggag tgctggaagc atgttgacca ccgggaacat
     1801 tttaatgcca cagatgcgta atccggttgc cagcagcaat aaaccgccta cggcgctgaa
     1861 gtctgccatc atcgacggtg tggtcagcgg taatatcagc gcggcagccc acgccagcgt
     1921 taactggatg atcagtaatg ggatactaat caccgatacc gcaataccca gcgagcaggc
     1981 gaagatcatc gccgtaaaga aatcaagaaa tgacttggcg attaaaatac tcggatcgcc
     2041 ggtcatccct tcgttcatcg ccccgaagat cccggtgccg ctggcgcaaa acaggacaat
     2101 aatcgcgaca taattctgaa taaaagattc atgcgctggc ttcttacgtg agtgacgaaa
     2161 cagattttgt gctttggcga ccgctgtatt gacgcctttc tccagcagac aaatttcgcc
     2221 gattaatgct ccgagtaagg ttgctaaaac catcgccgga aggttggcgc atttcaccac
     2281 cagtaaaata ccaatcccca gcgatgccaa accaaaaatt gatgtcatgg agacgcggat
     2341 acgttccggt aagcgttggc taagcagtgc accgagcatg ccacccagta atattgcact
     2401 tgcgttgata aaagggccga tgaccacaag agttcctgtt aactgtattg tttgattttc
     2461 ttattatgaa ctttttgcct tgttgatggc ttgtctggtc gcaaagtcct ggcttgcacg
     2521 ccttagcgaa aggtgccatg attgcgcgaa ttttctcctc tctgtacgga gtttgcccga
     2581 tgcacgccat ctccttacat tctctcgctt atcgccgttt cgcgcgaaac ccttctcttt
     2641 ttatgctact gctcatgcgg tgaagttaac gcacgctcac tgcaggacaa cagtaaaatc
     2701 agagcctttc tgcttttact gatgtctggc ggtcggagct ggtgaccagt ttgacccata
     2761 tctcatgggg cagggtttta caccttgtcc agtattctta cttccccgaa acgggtttgc
     2821 gcttatgaaa tcaatgaata ttgccgccag tagtgaactg gtatcccgac tttctactca
     2881 tcgccgcgtg gtggcgttgg gagatactga ttttacggac gtcgcggcag tcgtcattac
     2941 cgctgcggat agtcgcagtg gcattcttac gttgcttaag cgcaccggtt ttcatctacc
     3001 ggtgtttttg tattccgaac atgctgttga attacctgcg ggcgttacgg cggtaatcaa
     3061 cggcaacgag cagcagtggc tggagctgga atccgcagcc tgtcagtatg aagagaattt
     3121 gctgccaccg ttttatgaca cgctgacgca gtacgttgag atgggcaaca gcacctttgc
     3181 ttgccctgga catcaacatg gtgcgttctt taaaaagcat cctgccggac gccattttta
     3241 cgatttcttt ggtgagaaca tctttcgcgc cgatatgtgt aacgctgacg taaaattggg
     3301 cgatctgctt attcatgaag gatcggcgaa agatgcgcag aaattcgcag ccaaagtctt
     3361 tcatgccgat aaaacctatt ttgtgctgaa cggcacatcg gcagcgaata aagtggtgac
     3421 gaatgcgctg ttaacgcgtg gcgatctggt gctctttgac cgtaacaacc ataagtcgaa
     3481 tcatcacggc gcgttgattc aggcgggggc gacgccggtc tatctggaag cttcacgtaa
     3541 cccgtttggt ttcattggcg gtattgatgc gcactgtttt aatgaagagt atctgcgcca
     3601 gcaaattcgc gacgttgcgc cagaaaaagc cgacctgccg cgcccgtttc gcctggcgat
     3661 tattcagctg ggaacctatg acggcactgt ctataacgcc cgtcaggtga tcgataccgt
     3721 tgggcatctg tgtgattaca ttctgtttga ttccgcgtgg gtcggttatg aacaatttat
     3781 cccgatgatg gcggatagct cgccgctgct gttagaactt aacgaaaacg atccggggat
     3841 ctttgtgact cagtcggtgc acaaacagca ggcgggattc tcacagacgt cgcagatcca
     3901 taaaaaagat aaccatatcc gcggacaggc gcgtttttgc ccgcataagc ggttgaataa
     3961 cgcctttatg ctccatgctt ctaccagccc tttctatccg ctgtttgctg cactggatgt
     4021 taacgccaaa attcatgaag gggagagtgg gcgtcggctg tgggctgagt gcgttgagtt
     4081 gggtattgag tcgcgcaagg ctattcttgc gcgctgtaag ctgttccgcc cgtttatccc
     4141 gcccgttgtt gatggcaaat tgtggcagga ttatccgaca tcagtgttag ccagcgaccg
     4201 tcgttttttc agttttgagc cgggggcgaa gtggcacggc tttgaaggat atgccgcgga
     4261 tcagtatttt gttgatccgt gcaagctgtt actcactaca ccaggtatcg atgccgaaac
     4321 cggcgaatat agcgactttg gcgttccggc gacgattctg gcgcactatc tgcgtgagaa
     4381 cggcattgtg ccggagaagt gcgatctcaa ttccattctg ttcttattaa ctccggcgga
     4441 aagccacgag aagctggcac aactggtggc gatgctggct caatttgaac agcatattga
     4501 ggatgactcg ccgctggctg aggtgttgcc gagcgtttat aacaaatatc cggtgcgcta
     4561 tcgcgactac accctgcgcc agttgtgtca ggagatgcac gatctgtatg tcagtttcga
     4621 cgtcaaagac ctacaaaaag cgatgttccg ccagcagagt ttcccgtcag tggtgatgaa
     4681 cccccaggat gcgcatagcg cttatattcg cggtgacgtg gagttggtgc ggattcgtga
     4741 tgccgaaggg cgaattgcgg cagaaggggc gttgccttat ccacctggcg tgctttgcgt
     4801 ggtacccggg gaagtctggg gtggggcggt tcaacgttat ttccttgcac tggaagaagg
     4861 ggtgaatttg ttgccgggat tttcgccgga gctgcaaggt gtttatagcg aaaccgatgc
     4921 ggatggcatg aaacggttgt acggttatgt gttgaagtaa gaataaaaaa aacgggtcgc
     4981 cttctggcga cccgtttttc tttgcgtaat tagtggctaa ccgtctgtgt acctgtcggg
     5041 acacgaacgt gtttatattt gaacatcgcc atgaacgcga aggccagaac cacggagtaa
     5101 ccagcgaaaa tcaaccatac ggtctgccag tcggtaatac cgttttgggt gtacatctca
     5161 acaactttac cgctcacgat gccgccgagg atacagccga agccgttagt catcatcagg
     5221 aacatccctt gtgcactggc gcgaattgcc gggctaactt ctttttcgac aaacaccgaa
     5281 ccagagatgt tgaagaagtc gaacgcacag ccgtaaacaa tcatcgatag aaccagcagt
     5341 acggtgccga acggagtcgg gtcgccgtaa gcaaacagcg caaaacgcag gatccacgcc
     5401 acaatactga tcatcattac gttcttaata ccgtagcggc ttaagaagaa cgggatggtc
     5461 agaatgaaca gggtttcaga gatctgcgaa atcgacatga tgattgacgc atgctgcaca
     5521 ataaagctgc tggcaaacat cggatctttg tcgaagctgt gcaggaaggt attaccgaac
     5581 atgttggtaa tctgcagttc cgcgcccagc agcattgaga agatgaagaa gattgccata
     5641 cgcttgtttt taaacagcgc gaatgcatcg aggcccagca gggttgtcca gctctgattc
     5701 gcttgctgtt tagcaaccgg aatatgcggc agagtcaggg taaacagaac cagaatggcg
     5761 gaaagtgctg cgccaatata cagctgcatg tggcttaatt cgaagccaga caggctcacc
     5821 acccacattg ccatgataaa gccgatggtg ccccagatac ggattggcgg gaagtcagta
     5881 acgatatcca tcccggcatt ttgcaggcga tagtaagaga tggtgttgat taacccaagc
     5941 gttggcatat aagcaaacga gttaatcaat atcacaagga acatcgcctc cggcgtcgtg
     6001 acctgtgccg ccatgaacag cgtgatagcg ccaatggtgt ggcaaatggc atatacccat
     6061 ttcgcactta accatttgtc ggccacaatc cccagcagcg caggcataaa gaccgcggcg
     6121 atacccagtg agctataaac tgcgccaata gaagcaccgt caaacttcag ggtaacaaac
     6181 atataggaac cgagggtcgt cagccaactt ccccacagac agaactgcag aaaagagagg
     6241 attttcagct gcagcttaag attcatgtta atttcctcac atcgtgatgc ggatgagtgt
     6301 ttgaaaaagc acatttgacc gtgcgagatg atgggattct atcaacttcg cggactggtt
     6361 ttttgttacc tgtggcaaat aattgcaaac attcctaatt tgcaaaacga aattgtgata
     6421 tggataacat ttttgcccct gagcatcgtc aggggcggtt agtggaatta tcggcggcgg
     6481 taggattttt gcgcggtatt cactttataa agataacggc gagattccgc agagggatgg
     6541 cgggtcgtca gcgtttgata aacatcgccc ggcgtcatgg tgttaataat attagccgcc
     6601 tgaatcttat cattcgaaaa gactcgcagc acgctgcctg cgccgccgtt ataggcggtg
     6661 atgacggcat aacgccggga tgtagggtta tcaattccgc cgagataaac attgttcagc
     6721 atcgccagat acgcggtgcc ggtatcaata ttgctggcag gatcaaacaa gaaactgcgg
     6781 ctcggcgtgc cggatttccc ctgcgagcgg aacacatctt tcccggcagt atgttgtacc
     6841 acctgcatta accccagcgc atcggaacgg ctgaccgcat acgggttaaa ggaagattca
     6901 gtctgcataa ttgccagaat cagcgactca tcaacgccat atttccgtga cgcctggcgg
     6961 accatgccga gatatttgtg cgcacgttta tcaaggtggt tcggcaccat gttaatggtg
     7021 acgctgtaga tgatacgcag cccgttgctg cggctcttca gacggttttt cagcagataa
     7081 tccgcgaagt tgcttgcgcg accttcccag cgaatcggct gcccggtgtt gtccaccacc
     7141 tgaccgtaaa ggaaaggttc tttcgaaatt gtaatatcat caacgtcgga atagagatcg
     7201 accgaactcg gatcgtcacc catcagcaac gttttgataa ttgcccggcg caaatgcgcg
     7261 gcaggttctg tcccggcgat ggtttcgata gtaatcgtac cgtcatcgaa gttgatgtgg
     7321 ctgcgggtct gatattgatc ggtgtatttc acgtagtcct taggaccggc gatcaccacc
     7381 tctttgaatc cccagatgtt ctcaatattg tgggcaaatt gccccatcag aatatcaaaa
     7441 ccgttggtgt ctttgaccca ggcttcgtta taggtaccgc cttttttggt cgtcgaacag
     7501 gagatgagca acggcgcaat caaagccagc gcgagatatt ttttcatcat tccgggtgcg
     7561 tgttgtgttt gtttttatgc aagctgccgg aggcgtgctc cggcactgtt tttatttttt
     7621 atcttccggc gtatagccct cgatatgcac ctctttaccc tcgaacagga agttgaccat
     7681 ctcctgctcc agcagcttgc ggtgctcggc attcatcatg ttgagtttct tttcattaat
     7741 cagcatggtt tgtttgtgct gccactgcgc ccaggcttct ttggagatct cgttatagat
     7801 gcgttttccc agttcgccgg ggtacagctg aaaatcctga ccttctgctt cacgttgcag
     7861 gaaagtacaa aaaatcgttc tgctcataaa tcatcctctt tatcgactca cgcgctaaac
     7921 cggcgcgcca gtgcgtaact gctgtaacaa acgctccacg ggagccgcca ggccaactga
     7981 cggcggttgc gctaagttat accagagcgc attgccttca tccatgcagc cggtgaatga
     8041 cgacacggga agccacatag gcacaatatc taagtggaaa tggctgaagg tatgccgaaa
     8101 cgcggtcagc tgcgtcaggt tatcggcagc aatctgccgt tgcgccagcc actgccgcaa
     8161 actttcttcg tcggcaaact gcgggaaaca gtataaaccg ccccacaatc cgctcggcgg
     8221 acgctgcgcc agcaatactt catcttcgtg ctgtaacagg agaaagtagc cggtgcgctc
     8281 cggcagcgtc tgtttcggtt ttttgcccgg ataaagcgac cagctattgt tggtggcggc
     8341 aatacatccg ttttgtagcg gacagagcga acatttcggc ttcgaacgcg tgcaaatcat
     8401 cgcgcccaaa tccatcatcg cctgattaaa ccgttccacg ccgaccgcgg gcgtcacctg
     8461 ctcgcttaaa ctccataatt tattctcgac ctcttttttc ccaggccagc cgcttacagc
     8521 atagcagcgc gccagcaccc gtttgacgtt accgtcgaga atcggaaagt gcttacccag
     8581 agaaagcgat agaatcgcgc ctgcggtgga acgcccgacg cccggcagtg ctgcaacttc
     8641 ctcaaaggtt tccgggaatt taccgccgtg taaggtcgcc acttgttgtg ccgctttatg
     8701 cagattgcgc gcgcgggcgt aatagccaag ccccgtccac aagtggagaa cttcgtcgag
     8761 cggcgcattg gcgagatcgg tcaccgtcgg gaagcgcgcc ataaagcgtt caaaataggg
     8821 gataacggtc gcaacctgag tttgttgcaa catcacttct gagagccata ctttgtaggg
     8881 cgtcttgtca atttgccagg gcagagtttt tcgcccgtat ttatcgtacc agtccagaac
     8941 ctgggctgaa aattgcgacg cttgcatggt caccgaattc actgttgttg ggggcaagat
     9001 tgcagcacag cgacggcagg gtgtaaaccg gaactttccg cagccacggc cttatcatgc
     9061 ttgcatccgg caactaactt tggataatgc ccgttttcag aacactttca caagcgacta
     9121 aatctttatg aaaaacgacg tcatttcacc ggaatttgat gaaaacggcc gcccactgcg
     9181 ccgtatccgt agttttgtgc gccgccaggg gcgactgacc aaaggccagg aacatgcgct
     9241 ggaaaactac tggccggtga tgggcgttga gttcagcgaa gatatgctgg atttccccgc
     9301 gctttttggc cgtgaagcgc cggtgacgct tgagattggt tttggcatgg gggcgtcgct
     9361 ggtggcaatg gctaaagatc gccccgagca ggacttcctc ggcattgaag tgcattcacc
     9421 gggcgttggt gcgtgcctgg cttctgcgca tgaagagggc ttaagcaacc tgcgcgtgat
     9481 gtgtcacgat gcggttgaag tgctgcataa aatgattcct gacaattcat tgcgcatggt
     9541 gcagctcttt ttccctgacc cgtggcacaa agcgcgccat aataaacgcc gtatcgttca
     9601 ggtgccgttt gccgaactgg taaaaagcaa actgcagctg gggggcgtat tccatatggc
     9661 gaccgactgg gaaccttatg cggaacatat gattgaagtg atgtcttcta ttgacggtta
     9721 taaaaacctg tcagagagca atgattacgt accgcgtccg gcatcacgtc cggtgacgaa
     9781 atttgaacaa cgtggtcatc gtcttggtca cggagtatgg gacttaatgt tcgagagggt
     9841 gaaataatgg caaagaaccg tagccgtcgt ctgcgtaaaa aaatgcacat cgacgaattc
     9901 caggaattag gattttcggt ggcatggcga ttcccggaag gtacatcgga agaacagatt
     9961 gataaaaccg ttgatgattt tattaacgag gttatcgaac cgaacaaact ggcctttgac
    10021 ggcagcggtt atctggcctg ggaaggtctg atctgcatgc aggaaatcgg caaatgcacc
    10081 gaagaacatc aggcgattgt gcgtaagtgg ctggaagagc gcaacctgga tgaggtacgc
    10141 accagcgaac ttttcgacgt ttggtgggac taagaaagca tacgggcgat gacaaatgca
    10201 aaactgcctg atgcgctacg cttatcatgc ctacaaagat gcacgatcga gtaggcggga
    10261 taaggcgttt acgccgcatc cggcatggaa aacgcgtact ttgttatcaa tctggggcca
    10321 gcaaatgctg gcctgatttg ttcttgaggg aagactatga tgcgcaaaat gctgctggcg
    10381 gcagcacttt cagtgacggc aatgaccgct cacgccgact accagtgcag cgtcacgccg
    10441 cgtgacgatg tgattgtcag cccgcaaacc gtgcaggtga agggcgaaaa cggcaatctg
    10501 gtgatcacgc cagacggcaa cgtgatgtat aacggtaagc aatattccct gaatgccgcc
    10561 cagcgcgagc aggcgaagga ttatcaggct gaactacgta gcacgctgcc gtggattgat
    10621 gaaggcgcga aaagccgcgt cgagaaagcc cgtattgctc tggataaaat tatcgttcag
    10681 gagatgggcg aaagcagcaa aatgcgcagc cgtctgacca aacttgatgc gcagctgaaa
    10741 gagcagatga accgcattat cgaaacgcgc agcgatggcc tgacgttcca ctataaagcc
    10801 attgatcagg ttcgcgctga aggccagcaa ttagtgaatc aggcaatggg cggaatttta
    10861 caggacagca ttaatgaaat gggcgcgaaa gcggtgctga aaagcggcgg taacccatta
    10921 cagaatgtgc tgggaagcct gggggggctg caatcctcaa tccaaaccga gtggaaaaag
    10981 caggaaaaag atttccagca gtttggcaaa gatgtttgta gccgcgttgt gactctggaa
    11041 gatagccgca aagccctggt cgggaattta aaataatcct ctattttaag acggcataat
    11101 acttttttat gccgtttaat tcttcgtttt gttacctgcc tctaactttg tagatctcca
    11161 aaatatattc acgttgtaaa ttgtttaacg tcaaatttcc catacagagc taagggataa
    11221 tgcgtagcgt tcacgtaact ggaggaatga aatggagttt ttcaaaaaga cggcacttgc
    11281 cgcactggtt atgggtttta gtggtgcagc attggcatta cccaatatca ccattttagc
    11341 aaccggcggg accattgccg gtggtggtga ctccgcaacc aaatctaact acacagcggg
    11401 taaagttggc gtagaaaatc tggttaatgc ggtgccgcaa ctgaaggaca ttgcgaacgt
    11461 taaaggcgag caggtagtga atatcggctc ccaggacatg aacgatgatg tctggctgac
    11521 actggcgaaa aaaattaaca ccgactgcga taaaaccgac ggcttcgtca ttactcacgg
    11581 taccgacacg atggaagaaa ccgcttactt cctcgacctg acggtgaaat gcgacaaacc
    11641 ggtggtgatg gtcggcgcaa tgcgtccgtc cacgtctatg agcgcagacg gtccattcaa
    11701 cctgtataac gcggtagtga ccgcagctga taaagcctcc gctaatcgtg gcgtgctggt
    11761 ggtgatgaac gacaccgtac tggacggtcg cgatgtcacc aaaaccaaca ccaccgacgt
    11821 agcgaccttc aagtctgtta actacggtcc gctgggatac attcacaacg gtaagattga
    11881 ctaccaacgt accccggcac gtaagcacac cagcgacacg ccgttcgatg tctctaagct
    11941 gaatgaactg ccgaaagtcg gcattgttta taactacgct aacgcatccg atcttccggc
    12001 taaagccctg gtagatgcgg gctatgatgg catcgtgagc gctggtgtgg gtaacggcaa
    12061 cctgtataaa tccgtgtttg acacgctggc gaccgccgcg aaaaacggta ctgcagtcgt
    12121 gcgttcttcc cgcgtaccga cgggcgctac cactcaggat gccgaagtgg atgatgcgaa
    12181 atacggtttt attgcctctg gtacgctgaa cccgcaaaaa gcgcgcgtcc tgctgcaact
    12241 ggctctgacg caaaccaaag atccgcagca gatccagcag atcttcaatc agtactaatc
    12301 atttcgcccc ggtatcgcgc cggggctttt tcacttcaga cttacgtcca ttgccaattt
    12361 taattgccct aatgataatc accagaataa attattccgc gcgagggttt tcgggtgaaa
    12421 aagcaatgga ttgttggcac ggcgctgctt atgttgatga ctggtaatgt ctgggcagat
    12481 ggcgaaccgc caactgaaaa tatcttaaaa gatcaattca aaaagcagta tcacggcatt
    12541 ctcaagcttg atgccatcac cttaaaaaat cttgatgcta agggtaatca ggccacctgg
    12601 tcagcggaag gcgatgtctc ttccagtgac gatctctata cctgggtcgg tcagttggca
    12661 gattacgaac tgctcgaaca gacctggacg aaagataaac cggtaaaatt ctcggcgatg
    12721 ttaaccagta aaggaacgcc cgcgtctggc tggacggtga acttttactc ttttcaggcg
    12781 gcagctagcg atcgtggtcg ggtggtcgac gatatcaaaa cgaataataa atatctgatc
    12841 gtgaatagcg aagatttcaa ttatcgcttt agtcagcttg agactgcgtt gaatacccag
    12901 aagaactcga ttcctgcgct ggaaaaggaa gtgaaagcgc ttgataagca aatggtggcg
    12961 gcgcagaaag cagcggatgc atattgggga aaagatgcaa atggtaagca aatgacccgc
    13021 gaagatgcct ttaaaaaaat tcaccaacag cgtgatgagt ttaataaaca gaacgatagc
    13081 gaggcgttcg cggttaaata tgacaaagag gtttatcaac cggcgattgc ggcatgtcat
    13141 aaacagagtg aagagtgtta tgaagtgccg attcagcaga aacgagattt cgatatcaac
    13201 gaacaacggc gacagacttt tctgcaatca caaaaactta gccgtaaatt gcaggatgac
    13261 tggataacgc tcgaaaaagg gcaatatccg ttaacgatga aagtttcaga aataaacagt
    13321 aaaaaagtcg cgattttgat gaaaattgac gatatcaacc aggctaatga acgctggaaa
    13381 aaagataccg aacaactgcg gcgcaatggg gtgattaagt aagttttgat atagcgcacg
    13441 atttgtagca ggatgcggcg ttgaccccgc atccggcaat caacgtctga tgcgacgcta
    13501 gcgcgtctta tcagacctac aacagctgcc acatgtcggc tgaggtgctc acgccgcatc
    13561 cggcaataca agtttactca gccagaaaaa gctccagcag cgaatttaaa aacagcttcc
    13621 catgttccgt aatctgccag taatccgcac attcggtgag ataaccctgg gcaatcgcct
    13681 cgtctaactg tgggcgaatc acatcttcgc aaagcccggt atacgcacta aactccacgc
    13741 gcggcgcagg ttccagcaac cggaagcgat tcataaagaa ctcaaacggc ttatctgcgg
    13801 cttcgacatc acgctggctt tccagatacc ttccttgcat aaaaccacgc ggatgacgcg
    13861 ttttggtggt acgcagaatg cgcccatccg ggaaggtcac tttgccgtgc gcgccgcagc
    13921 caataccgat gtagtcacca aagcgccagt agttgagatt gtgctggcac tgataaccgg
    13981 gtttggcgta agcggaagtt tcatattgct gataacccgc tgcggttaat aactgatgcc
    14041 cctgttcgaa aatatcccac agcgcgtcgt cgtccggcag taccggcggg cgtgaaccaa
    14101 acagcgtatt aggttcgatg gtcagttgat accaggaaag atgcggcgga ttcagttcaa
    14161 tagcctggcg cagatcgcca agcgcctctt ccagcgattg atccggtagc ccatgcatca
    14221 aatcaaggtt aaagctacgt aaccctaaac cgctcgccag ctttgccgct cgtttcgctt
    14281 cttgcgggcc atgaatacgt cccagccgtt tcagcttttc ttcgctaaaa ctctgtaccc
    14341 caatagagat acgatttaca ccggcacgct gataatcaac aaagcgatcg gcttctaccg
    14401 taccggggtt cgcttccatg gtaatttctg catccgctgc cagcggcaaa cgcgcacgca
    14461 cgccgtccag cagcgtttgc atcgccgagc cggaaagcag gctcggcgta ccaccgccaa
    14521 taaagattgt ctttacttca cggccctgag cgtaagccac atcgttgtcc agatcgttaa
    14581 gcagatgctg aacgtaatcg tcgtgcggca cttctccttt caacgcgtga gagttgaaat
    14641 cgcagtacgg gcatttctgc acgcaccacg ggatgtgaat gtagagactc agcggcggta
    14701 atttaaccat tacgtaaagc gtccagcagc agtttcaatg cctgaccacg gtgggaaatg
    14761 gcgctctttt cttcgcgggt cagttcggca gcggttttcc cttcggaagg tacgaagaag
    14821 attggatcat aaccaaagcc accagtgccc gccggttcgc gagtaatcac acccggccag
    14881 ctgccatggc acaccagcgg ggtcggatct tctgcgtgac gcagatacac cagcacgcag
    14941 tggaaacgcg cctgacgttg gtcgtccggt acgtctttca gtgtttccag cagtttttgc
    15001 agattctttt gatcggtcgc gtcttcaccg gaataacgtg cggagtaaat acccggcgcg
    15061 ccgccaagca catctaccgc caggccagag tcgtcggcaa ttgccggtaa accggtcact
    15121 tttgccgcgt ggcgcgcttt cagaatcgcg ttttcgataa aggtcaggcc ggtttcttca
    15181 gcggaatcaa cgccgagttc tgtttgggcc acgatatcaa gaccgaagtc gctaagcagc
    15241 gacgccagct cacgcacttt accggcattg ccggttgcga ggacaacttt ttgcatagga
    15301 tacctaatta attaacgccg cgacttctgg cgggatttgt tgcggattaa tgattttaat
    15361 ttgtttgtgg cggccaagtt cgcctttttc aatcaccacc tggcttttgg caacccggaa
    15421 ttgcttaccg agaaacttca ccagatggct gttggcctga ccgtcaaccg gcggcgcggt
    15481 aatggcgact tttacctcgt cgccatgtaa accgacaata gaatcacggc tggctttcgg
    15541 ctgaatatag agccgtaaaa ccagaccgtc atcgttaact gttacggcac tcataacgcc
    15601 atccacagcc ccggcagcag catatttccg gtcgcctgta atacttctgc gacacccata
    15661 ttgatgacat acagcagcag aacgaggatc atcggcgaga aatcaatccc ccccattgcc
    15721 ggtagcaagc ggcgaatcgg gcgcagcagc ggatcggcca gctgaatcag cacgtattca
    15781 atcgggctac ggccctggct tacccagctc ataatcgcca tcaccagcag gacccagaaa
    15841 atcagcaggc cgatggtttt cagcagaatc agtaaaccgg caatccagat gattggcagg
    15901 aaggtcacca ctttaaacag cacgatggct ttgataaaac tgagaatata ggcaaccagc
    15961 agcgaggcgc tatcaatggg tcccattgcc ggaataacgc ggcgcagtgg cccgataatt
    16021 ggctgcgtta ccttcactac aaactgtgag aaggggttgt aaaaatcaca atgagcccac
    16081 tgcatccaga tgcgtaataa cagcaccatg gtatacagct caatgaccgt tgaaagcagg
    16141 aaagtcaacg tattcatggc gttcctttaa ttccttattt tttagagtaa tcacgcgcac
    16201 caaaaattgc agtgccgata cgaaccatcg tgctacctgc cgcaatggcg gcttccatat
    16261 cgtccgacat tcccagagag agcgtgtcga tatgcgggta gcgcgttttc agtccggcaa
    16321 atgctacagc catttggcgt gcaacttcaa actgccttac atattctgac tcaggcgcag
    16381 ggattgccat cagcccgcgc agacgtaaac gcggtagttc agcgaccgca gctgccagct
    16441 cgtccagttc tgccagttga atcccggact tactgttttc atcactaata ttaatttgaa
    16501 tcagtacgtt aagaggggga agttctgccg ggcgctgatc gttgaggcgg gtagcaatgc
    16561 gcaaacggtc gatggtatga caccagtcga aatgctctgc caccaggcgg cttttattag
    16621 actgcaacgg gccaataaaa tgccattcta atcctgttac gcccagttcc tgaaagtggc
    16681 gaattttatc taccccttcc tgaacgtagt tttcaccaaa ttgacgctgc ccggcatcaa
    16741 tggcttctgc gatggcgctc gcaggttttg ttttactgac tgcaagcagc gtaatttctt
    16801 ctggagaacg gccgcaacgc gttgcagccg ctgagatttt gtcccggacc tgtgccaggt
    16861 tatgcgcaat atcgttcatt ttccgaggat cttagtatga atatggaaga aattgtggcc
    16921 cttagtgtaa agcataacgt ctcggatcta cacctgtgca gcgcctggcc cgcacgatgg
    16981 cgcattcgcg ggctaatgga agctgcgccg tttgatgcgc cggacgtcga agagctactg
    17041 cgggagtggc tggatgacga tcagcgggca atattgctgg agaatggtca gctggatttt
    17101 gccgtgtcgc tgacggaaaa ccagcgattg cgtggcagcg cattcgcgca acggcatggc
    17161 atttcgctgg cgttacggct gttaccttcg cactgcccgc agctcgaaca gcttggcaca
    17221 ccaccgatat tgccggagtt actcaagagc gagaatggct tgattctggt gacgggggcg
    17281 acggggagtg gcaaatctac cacgctggcg gcgatggttg gttatctcaa tcaacatgct
    17341 gatgcgcata ttctgacgct ggaagatcct gtggaatatc tctatgccag ccagcgatgt
    17401 ttgatccagc agcgggaaat cggtttgcat tgtatgacgt tcgcatcggg attgcgggcc
    17461 gcattgcggg aagatcctga tgtgattttg ctcggagagc tgcgtgacag cgagacaatc
    17521 cgtctggcgc tgacggcggc agaaaccggg catctggtgc tggcaacctt acatacacgt
    17581 ggtgccgcgc aggcagttga gcgacttgtg gattcatttc cggcgcagga aaaagacccc
    17641 gtgcgtaatc aactggcagg tagtttacgg gcagtgctgt cacaaaagct ggaagtggat
    17701 aaacaggaag gacgcgtggc gctatttgaa ttgctgatta acacacccgc ggtggggaat
    17761 ttgattcgcg aagggaaaac ccaccagtta ccgcatgtta ttcaaactgg gcaacaggtg
    17821 gggatgataa cgtttcagca gagttatcag cagcgggtgg gggaagggcg tttgtgacgt
    17881 tgccatgccc gatgcgcaag cttatcgggc ctacaaaggc aataaacgtc tgatgcgacg
    17941 cttgcgcgtc ttatcagacc tacaaccgct gccacatgta ggccggataa ggcgttcacg
    18001 ccgcatccgg caataaggcg taaggcgcga tgatattatt tacactccag caggcgatta
    18061 aatctcctta atatccctgc tcgaaatagc tttcgagaat aataaccgca gaggcagagt
    18121 cgattttgcc tttgttgagc gcccgatagc cgccctgttc aaacagaccg gaacgtgcct
    18181 ccacggtgct aagacgctcg tcatggagct ttacttcaac accgaaacga ccatgaatac
    18241 ggttagcaaa tttacgcgct ctggcggtta acggctgttc ggtgccgtcc atattcagcg
    18301 gtaaaccgac gatgatttcg tctggctgcc actctttcag taaacgttcg atgaggttcc
    18361 agtccggcgt accgtcctgt gctttaattg caggcaaagg gcgagcggtg ccggtaatgc
    18421 gttggccgac cgctacgcca atgcttttgg tgccgaagtc gaaggcgagt aaggttccac
    18481 tcatcaggcg tgtcctgcca caccaggcat ggtgagaata tccacaccaa tcagttttgc
    18541 cgcctcgcgc cagcggtcgg caattggcgt tttgaacaga atattcagat ctgccggggc
    18601 ggttagccac gcgttatcga gaatttcttg ttccagttga cctttttccc aggaggcata
    18661 gcccagagcc accaatacgt cagacggttg tttatcggtg ccgagcgttt ccagcacatc
    18721 gcgggaagtg gtcatcactg tgttgtctga aatgcgaatg ctggaagcaa aattggaggg
    18781 cggagtatgc aaaataaacc cgcgatcttc agccagtggg ccgccgagca taaccggttt
    18841 atccagacgg attgattcat cacgcggctc cggcgtgatc ttcagctttt ccagaatccc
    18901 ttcaattttg agattttcca gcggcttgtt gacgatgatc cccattgcac cattggtatt
    18961 atgttcgcaa atgtagacca cggaacgacg gaaaatcgga tcctggagag caggcatggc
    19021 aataagaaag tgatgctgta aattcattgt cagaggttct gtttcctggt tcaaaaaagc
    19081 aacagcgccc agtatgcgtg gaaagaaggg cgctgtcact cagagtctca acgagatcct
    19141 tctcgctaag gtgggttact actgctgtaa acgtgcttcg atggcatcca ttaacattcc
    19201 ggtgatcgac accggaaact ctgcttcaat ctcacgaata caggttgggc tggtgacgtt
    19261 aatttcagtc agacggtcgc cgatgatatc cagaccaaca aaaatcagcc ctttttcttt
    19321 cagcgtcggc ccgatctgac gggcgatttt ccagtcactt tccgtcagcg gacgaggttc
    19381 accgcgacca ccggcagcca gattgccacg ggtttcgccc ccctgcggaa tacgcgccag
    19441 gcagtacggt actggttcgc cgtccaccac cagcacgcgt ttgtcgccat ctttaatggc
    19501 tggcagataa ttttgtgcca tgcagtagcg agtgccgtgt tcggtcaagg tttcggcaat
    19561 gacgccgagg tttggatcgc cttctttcac gcggaaaatc gacgcgccgc ccataccgtc
    19621 cagcggctta agaatgatgt cgctgtgttt ctcccagaac gcttttagct gcgctttatt
    19681 gcgcgtaacc agcgtttctg gcgttaagtc agagaaccag gcggtaaaca gtttctcgtt
    19741 acagtcgcgc aggctctgcg gcttgttaac gatcagcgtc cctttctctt cggcacgttc
    19801 cagaatatag gtcgcgtaga taaactcggt atcaaacggc gggtctttac gcatcaggat
    19861 cacatcgaga tcggccagcg gcagatcctg ttcaccgacg aatgaaaacc actcttcgta
    19921 gttctgtttc acgttcagcg tgcgggtatg ggcgcgggct tcgccattga tcagatacag
    19981 atcgcccatc tccatatagt gaagttcgta accacgacgc tgtgcttcca gcaacatagc
    20041 gaaactggaa tctttcttga tgttgatgtt tgcgatgggg tccatcacga tgccgagctt
    20101 gatcattatt cttctccgtt agcccaaatc gccaaatcgt acttgtagcg cggtaatggc
    20161 ggtgagcgca gttgtctctg tacgcaaaac gcgaggtccc aacaggatat cagtaaattg
    20221 atagcgggca gtcatggcaa tttcatctgc cgataaaccg ccttccgggc caatcagcag
    20281 gcggacgcgt tcaaccggta aaggcaacgt attgatgctg ttactggcgc gcgggtgaag
    20341 attcagtttc agtccttcat cctgctctgc acaccaggct tccagatcca tcgctggacg
    20401 gatttccggc acccggttac gaccacactg ctcacaggca gcaattgcaa tcttctgcca
    20461 ctgctggagc ttcttgttca gacgttcact atccagttta acgccgcagc gctcagaaaa
    20521 aagtggcgta atgaggctta caccgagttc gatcgatttc tggatcgtaa attccatttt
    20581 ttcaccacgc gacatcacct gaccgaggtg aatatgcagc ggagattcgc gatcgtcgat
    20641 ctggccttcc agcaccttca cttccacgct ttttttgctg gcgctggtaa tttcggcgtc
    20701 aaagacctgg ttgctgccgt caaacaattg caacgcctgt cccggcccca tacgcagtac
    20761 gcgcccaata tggttggcgg catcttcgca aagcgcgatg tgagaatggc tggtcagtgg
    20821 ttcaggatga taaatgcggg ggatacgcat agttaaaaat ccgcgtcgtc tccccacgcg
    20881 gttgtacgcg tggggtaggg gttaacaaaa agaatcccgc tagtgtaggt tagctctttc
    20941 gcgcctggca agcgcgttgc acatacgggt tatgattgcc ctgtaccttc gcgatgcgtt
    21001 catcgcgctc gcactcccag tcggtaaccg gatacatctt gtcccatgcg ttgaacagct
    21061 gcgtttgctg gcgagagagt gtcaggttgt aatggtcacg catatagaag taggtgcgcg
    21121 caatggcacc gcgtgcacgc gctggtggtt cggcagcttt ttctttgaaa tcgaccttca
    21181 tggcgcattg accgtactgg ccttcaccgc cattccactg gctgtacata aagttgccgc
    21241 gatcgccatt cacctcgccg actgacggtt gcaggttatg catatcgctt tccatcttgc
    21301 gatagaccgg atctttggcg caatttttac gtccaccgtc ctgccagcac tggcgctggt
    21361 gaccgaattg ccaggcggga acgacgtgtt cccactctac gcggctggcg cggttttcat
    21421 ttttgcgcac ctgatagccg cacgattgca gatcgacaac gccttttttg ccctgccagt
    21481 taattttaca tccgcaataa aacgtaccgg gcgcgtcagc gtggactttt accgccgcgg
    21541 ctttcgcctg agaaaaacta ttgatacctt cggccaacgc cgggccggaa aatgctgcgc
    21601 tcagtaccac cgcagcaata gacaaataac ggtacatcac ttactccgtg ttaaaacgag
    21661 ccagcaacgt agcgaaagtt gttttggtat gcaatcagcc agatcaggaa agttcctgat
    21721 agttcagatg gttattttgc gaccagttgt tcgccgcagt gaacacagcg atagacggct
    21781 tcgccacgaa ctacgcgatt atgacggcgt acggtaagct gatgttcctg acacttacag
    21841 ctgtagggga aggtgttgcg gcgcacggat tgcagttcga actgatgcgt acggcgggct
    21901 ggaacgccca gcacactttc catcatccac ttccactctt tgccatgtgg tgctacgcgg
    21961 ccaaaatgtt tccataccag caaatgtgcc agttcgtgcg gtaccacttc ttcaataaag
    22021 gcttcactgt tttccaacag caaaacggga ttaaggcgaa tttcatagct ttccagccag
    22081 gctgttccgg cagaggttcc gcgctgggtg taagagagtt ttggctccgg gtagttacgc
    22141 cccaatttca ggttggcctg ggcgagtttt tcccgcaggc gacgcataac ggcctgctgg
    22201 atggcgatag ggagacggga tgttttcata gcggcagagg atagagcgaa gaaaagatga
    22261 ctgcaagagg tggcttcctc cacgacggga ggaagcctgg ggagattaat cgtgagcgcc
    22321 gatttcgcgc agtttacgac ctttcatcag attacgttca atatgttcca gcgaaacgtg
    22381 tttggtttct ggcaccagcc acagtgtcag caggataaac aggacgttca gacccgcgta
    22441 aacccagaag gtattagcgt tgcccagcgt gttgagcatg gtcaggaacg ttgcgccaac
    22501 gatcatgttg gcaatccagt tggtggcggt ggagcaggtg atgccaaaat cgcggccttt
    22561 cagcggctga atttcggagc acagtaccca aatcagcgga ccggcactca tggcaaaacc
    22621 gacaataaac atcagcagca tggcgatggc gaaatactgc gccgacggag agtggatacc
    22681 gatatgcatc attgtaccga gtacgcccat accagcagcc atcaccagga agcccagcgt
    22741 tagcgttggt ttacgtcccc agcggtcaac aaggccgatt gcgataaagg tggcaagtac
    22801 gttggtcagg ccgacaatca ctgtccccca catttgctcg gtggtgttgg tataacccgc
    22861 cagttcgaag attttcggcg cgtaatacat gatgacgttc atcccggtga attgctgcat
    22921 tacctgtaac agtacgccaa ggaacaccgc gcggcggaag ttgctattct ctttaaacag
    22981 cgcccagcca ctctgtttaa cctgcaaact ttcacggatt tcatccagtt cgcgtttcgc
    23041 ttccgcgctg gtgtcacgca ggcgtagcag cacgcgttcg gcatcaacaa aacggcgttt
    23101 ggcggcaaac caacgtgggc tgtctggcag gaagaagaca ccaatcagca gcaaaattgc
    23161 cgggatgata atcacaccca gcatccagcg ccatgcaccg gtgtagctga aggcggtatc
    23221 agaaagataa gcaccgagga tcccgattgt gatcattaac tgatacatcg agatcatact
    23281 gccgcgaatt ttttccggcg caatttcaga gaggtacagt ggtgcggtat aagaggccac
    23341 acctaccgcc agccccagca gaacgcggga aagaatcagt acttcaacgt ttggcgcagc
    23401 cgcagagaac agcgaaccgg caacaaacaa aattgcgccg atcatcaggc tctttttgcg
    23461 cccgagttta aaggagagcc agccgctgcc caccgcaccg actgccgcac cgaacatcat
    23521 ggagcttacg acccattctt gcgtgtgcga agtaatctgg aattcatctg caataaacgg
    23581 cagtgcgcca gcaattacac cgatatccag gccaaagagt aatcccgcca gagcggcaag
    23641 gaagcagacg aaaaacgtca ttgccttgtt tgactgcccc tgttttttag cgtcaggcat
    23701 gatgccctcc aatatggtta ttttttattg tgaattaaga taggtgagta cgacgtaaaa
    23761 agatgtgaag caaatcacat cagtgtaatc gcttacacta atat
//
LOCUS       DAZKIP010000066        23800 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZKIP010000066 DAZKIP010000000
VERSION     DAZKIP010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23800
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.066"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            25..954
                     /gene="aaeR"
                     /locus_tag="ACHOUK_002841"
     CDS             25..954
                     /gene="aaeR"
                     /locus_tag="ACHOUK_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFP9898251.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            complement(1088..2533)
                     /gene="tldD"
                     /locus_tag="ACHOUK_002842"
     CDS             complement(1088..2533)
                     /gene="tldD"
                     /locus_tag="ACHOUK_002842"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFP9898252.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFVILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIARD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLIEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(2689..6489)
                     /gene="yhdP"
                     /locus_tag="ACHOUK_002843"
     CDS             complement(2689..6489)
                     /gene="yhdP"
                     /locus_tag="ACHOUK_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFP9898253.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKET
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLGIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPAAVNEALSGSVAWDGKVGIVLPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLPVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTQVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWSDAQIRCAECWFWGQKFGRIDSDITISGNTLTLTNGLI
                     DTGFSRLTADGEWINNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            complement(6557..8026)
                     /gene="rng"
                     /locus_tag="ACHOUK_002844"
     CDS             complement(6557..8026)
                     /gene="rng"
                     /locus_tag="ACHOUK_002844"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFP9898254.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            complement(8016..8609)
                     /gene="yhdE"
                     /locus_tag="ACHOUK_002845"
     CDS             complement(8016..8609)
                     /gene="yhdE"
                     /locus_tag="ACHOUK_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFP9898255.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKARAGVAQTAKDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSRHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYQAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            complement(8618..9106)
                     /gene="mreD"
                     /locus_tag="ACHOUK_002846"
     CDS             complement(8618..9106)
                     /gene="mreD"
                     /locus_tag="ACHOUK_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFP9898256.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            complement(9106..10209)
                     /gene="mreC"
                     /locus_tag="ACHOUK_002847"
     CDS             complement(9106..10209)
                     /gene="mreC"
                     /locus_tag="ACHOUK_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFP9898257.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSLQRATPPQSGAQPPARAPGGQ"
     gene            complement(10275..11318)
                     /gene="mreB"
                     /locus_tag="ACHOUK_002848"
     CDS             complement(10275..11318)
                     /gene="mreB"
                     /locus_tag="ACHOUK_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFP9898258.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            complement(11623..13563)
                     /gene="csrD"
                     /locus_tag="ACHOUK_002849"
     CDS             complement(11623..13563)
                     /gene="csrD"
                     /locus_tag="ACHOUK_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFP9898259.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            13715..14689
                     /locus_tag="ACHOUK_002850"
     CDS             13715..14689
                     /locus_tag="ACHOUK_002850"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFP9898260.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            15667..16137
                     /gene="accB"
                     /locus_tag="ACHOUK_002851"
     CDS             15667..16137
                     /gene="accB"
                     /locus_tag="ACHOUK_002851"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFP9898261.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            16148..17497
                     /gene="accC"
                     /locus_tag="ACHOUK_002852"
     CDS             16148..17497
                     /gene="accC"
                     /locus_tag="ACHOUK_002852"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFP9898262.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNSIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            17606..17848
                     /locus_tag="ACHOUK_002853"
     CDS             17606..17848
                     /locus_tag="ACHOUK_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFP9898263.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            17838..19289
                     /gene="panF"
                     /locus_tag="ACHOUK_002854"
     CDS             17838..19289
                     /gene="panF"
                     /locus_tag="ACHOUK_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFP9898264.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            19301..20182
                     /gene="prmA"
                     /locus_tag="ACHOUK_002855"
     CDS             19301..20182
                     /gene="prmA"
                     /locus_tag="ACHOUK_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFP9898265.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            20511..21476
                     /gene="dusB"
                     /locus_tag="ACHOUK_002856"
     CDS             20511..21476
                     /gene="dusB"
                     /locus_tag="ACHOUK_002856"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFP9898266.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            21502..21798
                     /gene="fis"
                     /locus_tag="ACHOUK_002857"
     CDS             21502..21798
                     /gene="fis"
                     /locus_tag="ACHOUK_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFP9898267.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            21883..22767
                     /gene="yhdJ"
                     /locus_tag="ACHOUK_002858"
     CDS             21883..22767
                     /gene="yhdJ"
                     /locus_tag="ACHOUK_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFP9898268.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLLEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLITK"
     gene            22851..23030
                     /locus_tag="ACHOUK_002859"
     CDS             22851..23030
                     /locus_tag="ACHOUK_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFP9898269.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(23033..23695)
                     /gene="acrS"
                     /locus_tag="ACHOUK_002860"
     CDS             complement(23033..23695)
                     /gene="acrS"
                     /locus_tag="ACHOUK_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFP9898270.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
BASE COUNT         5736 a         6347 c         5975 g         5742 t
ORIGIN      
        1 atcactttta agtcagagtg aataatggaa cgactaaaac gcatgtcggt gtttgccaaa
       61 gtagttgaat ttggctcttt taccgccgcc gccagacagc tacagatgag cgtttcgtcc
      121 atcagtcaga cggtgtcaaa actggaagat gagttgcagg taaagctgtt aaaccgtagc
      181 acacgcagca ttggcctgac cgaagccggt agaatttact accagggctg ccgtcgtatg
      241 cttcatgaag tgcaggatgt tcatgagcaa ctgtatgcct tcaataacac ccccatcggg
      301 acgctacgca ttggctgttc ttcaactatg gcacaaaatg ttctcgccgg gctgacagcc
      361 aaaatgctga aagaataccc aggtttgagc gtcaatctgg ttaccggaat tccagccccc
      421 gacctgattg ccgacggtct ggatgtggtg atccgcgtcg gcgcgttgca ggattccagc
      481 ctgttttccc gccgtcttgg cgcgatgcca atggtggtgt gcgccgcgaa aagctatctc
      541 acacaatacg gcataccgga aaaacccgcc gatttaagta gccattcatg gcttgaatac
      601 agcgtgcggc ccgacaatga atttgaactg attgcaccgg aagggatctc gactcgcctg
      661 atcccgcaag ggagatttgt gactaatgat ccgatgacgc tggtgcgctg gctaacggcg
      721 ggtgccggga tcgcctacgt gccgctgatg tgggtgatca acgagatcaa tcgtggggag
      781 ctggagatcc tgctgccgcg ttatcagtca gatccacgcc cggtttatgc gttatatacc
      841 gaaaaagata agctgccgct gaaggtacag gtcgtgatca actcgctgac ggattatttt
      901 gttgaggtcg gtaaattgtt tcaggagatg cacgggcgcg ggaaagagaa gtaattcata
      961 ttgtactgtt acgttgtaca aacctgtgcc aacgggttcc cctcacccta accctctccc
     1021 caaaggggcg aggggaccgt tcgtgcacga ggaaggatta attatccttc tgaaaatact
     1081 gaaattatta cgcagtaccg ccaacagtca ggttatcaac tttcagcgtt ggctggccca
     1141 cgccaaccgg caaactttgc ccttctttac cgcagacacc cacgccgtta tccagtttca
     1201 ggtcgttgcc aaccatcgaa atctgctgca tggtttcgat accggaacca atcaacgttg
     1261 cgcctttcac cggcttcgtt actttaccgt tttcaatcag atatgcttct gaagtggaga
     1321 aaacgaattt gccggaggtg atatccacct gaccgccacc aaagttcggt gcatagatac
     1381 cgtactcaac ggattcaata atttcctgcg gggtcgattt acccggcagc atataggtgt
     1441 tggtcatacg cggcatgggc agatgggcgt aggattcacg gcgaccgttg ccagtcggcg
     1501 tcatccccat caaacgcgcg ttgagtttat cctgcatgta gcctttcaga atgccgttct
     1561 caatcagcac gttgtactgg cctggcgtac cttcgtcatc aatcgccacc gaaccacggc
     1621 gatcgaccat cgtaccgtca tcaaccacgg tacacagttc tgaagccacc agctccccga
     1681 cctgtccact aaatactgaa gtgccacggc ggttgaagtc gccttccaga ccgtgaccta
     1741 ccgcttcatg caacagcacg cccggccaac ctgcgccaag tactaccggc atggtgcccg
     1801 ctggtgcagc aacggcagaa agattgacca gcgccatacg tactgcttct tttgcccatg
     1861 catccgcacg gacttcgccg tcgagatcgg caaggaagaa ctcataacca aaacgaccgc
     1921 cgccgccact ggcaccgcgt tcgcgtttgc catcttcttc gatcagaacg ctcacggaaa
     1981 gacgcaccag cggacggaca tccgccgcta gcgtgccgtc ggtggccgca accagaatta
     2041 attcatagac accactgagg ctggcagtca cttcctgtac gcgcttgtcc gcttcgcggg
     2101 caaccttatc gacgcgacgc aggatatcca gcttctcttc acggctcatg ctttgcagcg
     2161 gatctaccga ggtatacaac gggctatgct ctaccgcgcc cagcgtctgt actttgccat
     2221 caccgctatc acgggcgatg gtgcgcgccg cttgcgcact ctgttccagc gccagcaggc
     2281 tgatttggtc agcgtaagca aatccggttt tttcaccgct gattgcacgc acaccaacgc
     2341 cctgatcgat gttgtaagaa ccatctttaa taatgcggtc ttctaaaacc caggattcgt
     2401 gatagctcga ctgaaaatag agatcgccat aatcaaggcg acgttcggcc agttgaccga
     2461 ggatcacgaa caagtcctga tgtttcaggc cgttcgccgc tagcaattgt tcacttacca
     2521 ggttaagact catcgttttt gctactcgtt agttactgca gtagaggatt ttttacggct
     2581 gccggatgcg gcgtgaacgc cctatccagc ctacggttat gttctggctc gttggccttt
     2641 ggcaacgatt atcctatgag attggggcaa ttacgcgccc tcgtcaaatc attgcgcttt
     2701 ttctttacgc ggttggcgca acacttcgtt gatttgcgga tcgtccagcg gacccgaaat
     2761 gtgatagcgc aaaatggaga ctttgctcca cagcggcccc agcactttac tggcggcaaa
     2821 cactgccgcg ccaacaatgg ggttaaccgc aaaagccgca gccacgccca ctgtcgcaga
     2881 aatctctggt gcgacaaccg cttccatatt caggtcgcga cgtaccagat ttaccgaccc
     2941 tttcatggcg atatccgcct ccaggccatc caccagcgtg tcgtcggtgt gcataacgcc
     3001 gtctttaatc cacgcggtgc tgcgaatgga gtcaaaatag aacccttcgc caaaagtgtc
     3061 tctgaaatca aaacgcagct tacgcatcag ggcatctacg ctcaataagc gcagcaattg
     3121 cccggcatgt ccggtattga tttcggtaat ttcgccttta cccagttgag tatgaatgat
     3181 gccattcaac gtcgcctcat ctggctgcca cggtgcttta cgccagtgta aatcgtaatc
     3241 cacattaaat gacgactgcc gtatgggcgt cgtgacacca aaaaattctg cggcggcatc
     3301 aattttctgc ccgcgcagtt ttcctttcag cgaggtacgt tcattccccg gattgttaat
     3361 ccattcacca tcggcagtga gccgcgaaaa accagtatca atcagtccat tggtcagcgt
     3421 taatgtattg ccagaaatgg tgatatcact gtcaatgcgc ccgaattttt gcccccagaa
     3481 ccagcactct gcgcatcgta tttgggcgtc cgaccagcca cggaagttaa tgcgttccgt
     3541 tgtcgggaaa ggtgatgacg gcgttgaatc accacgagtt ttcgccacgc tcgggttgta
     3601 ataaagatat ttgatattcg ccagccacgg cgcgttatta cgcatcgcca gcgtggcgtt
     3661 gatttcacgc ccctgcgcct caacctgggt gccatttgcc gtcggttgcg aaacaatact
     3721 caggttattc cattgctgat ttcccagcga caacataggc gtacgtaacg ttatgtgttg
     3781 tgggaaactc gctgcaccac cgacactctc cgccgcacct ttctgaaaca gggccagcca
     3841 ctcggcacca ttcatcggcg gcatattgag ttcaacacca ctttgttccg gcaacggcgg
     3901 gagcgtttta ctgtctgccg cccaaatagc acggtcgagc gtcagctttt gaccgagcaa
     3961 ccagcggcta ttgaaatgat tatccgcacc agcctgtccg gttaattcaa agctgttgag
     4021 attgccatca accttaacgt ttaccggcag tggttcaccc gcaggtttgg ctaacggtga
     4081 aggtaagtga ctgctcacat tcttcaaatc gccgtttagc tctacgttat acgtcgcgcc
     4141 agcatgataa ggcagaacaa tgcccacttt accatcccac gccacgctgc cactcaatgc
     4201 ttcgttcacc gctgcaggca gaacgccggt tttcgccggt tgccagttac cgttgagatt
     4261 caccgcgacc tggtaggctt ttgccccttc tttggtggaa aaatccacgt tcaacggctg
     4321 attaaaccag cttgctgtca gtggttcact ttgcagatcg ccattgataa agctgaattt
     4381 accgctcaaa tttttcaggg tgctgtcgag tggtttgata aacagactgt tattacgcag
     4441 cgtcacttca cctttcgcgg ttaccagctc gccgttcagc gggatatcaa gatgtaagcg
     4501 agcattcaca tcgccgtcga gctggagttc ttgcagggtc gcacccagag aatctttcag
     4561 cggtgtctca tcaaagtaag ggccaacggc tttacccgga cctttaatgt cagcgtcaat
     4621 cagcagtttt tcttttgagt agtcagggat cactgcggtg agattactcg cgcgcacgcc
     4681 gcccagatta acgccatcgg ttttcatcca taaaccgtcg ttaataaagt ccagttcaat
     4741 accaaggtta gttaatgcag gccagtccgg ctggaaggca aacttcgcgt tgcgcagcgg
     4801 caccagcact tcaaactgac cttcgttgtg tttatagggg aagagttgcg gattgccacc
     4861 ataaaccagc gtcgcgttat ccgcttcacc gccctgaatc gcgccactta agtaatcaac
     4921 caggtcttta cccatcaagt tttccgggaa atagcgccag gcttgtgaac catcatcggt
     4981 actgatgcca gccagaatac ccagccaggg ttcatcgtta gcaggttgca ggtaacgaaa
     5041 accgccgcgc gcatggacgg ctttggcttt aacgtcaata ttacgcccat ccagctggaa
     5101 acctttgtca ttgttcagcc agcttatagt tgcctggccg tcggcgattt ccagtggcgc
     5161 acggaatacc gtttcgtaag gcatctttgc ctgcttcatc gacgcggtaa gcaaaccatt
     5221 ttcaacgctg ccggaaagcg tcccggagaa gtgttccgca cccggtaata atttccattg
     5281 cttccaggcc agatcgctcc acgatgcctg aaaacgggtc ttgtctgccg cctgaagcgg
     5341 gatatccagc gccagagtgt taatcttgcc gctcggttgt gtagagcgcc aaacatcacc
     5401 cagtgcaggt gaaagtttcg cggccagcgg gcgtatgccc tccaggcctg ccagctccag
     5461 attactggcg cgaatccgga gttcgtcact gcgtttattg tctttgccgc caacgtcctg
     5521 ttccggtatc caggccagcg tcaatgctcc gctcggccag ggtttgccgt ccatcgtgat
     5581 ccgtgtatcg ggaatagaga actgccatcc cggattttca cgcgtaatat gcgcggtcag
     5641 attatccacc gacagcgtat gcgtttcctt ctcgcccaac cagctggcac caccctgttt
     5701 cagccagacg tcaccgccgg ttacatcgcc tttgtcgatc gtcatccagc cttcaaggga
     5761 gaactgcgcc gtttccagcg caatattgtc ctgcatccat ttaccgagcc acggcttcag
     5821 gtcgatgtca tccgcctgga gccagacgcg accattgctt aacaacccct catcatcgcg
     5881 caaatccatg cgcacctgca tcacgccgtg ctgtccggta aggctggaga ggcttaccag
     5941 gccttccgca cggtgtcgac gtggatcgtt cagccaggtg agttgtggga tcgccagctc
     6001 ggcgcgctga ccggatggcg tcaggaaact gacttcactg tcgcggagat cgaaatggtc
     6061 aaattgacga agaaacagat cgctgatgtg actggcttcc agactgtcat cgctaccacc
     6121 gctggtgata ggagtgttgg tgcgaaagcg cagctgccag aaagtgaggt cgcgaaactg
     6181 ccagcgcata tgtaacaggc tctgccagac atccagcgcc agagtaacgc gtttaaccga
     6241 aaattcgccg ccatccttta gttctgcacg gatgtcgtgc gcttcaagcg tcgggccaaa
     6301 attctgccag ctggctgaga gctgactggc ttctaccggc atgccagtcg cggattctat
     6361 tttgttgagg atttccggac gccaggcgtc aagatgcggt aaagcaatac gcaggccgct
     6421 aaccagcagg gcagcgatca caacgagcgc ggctccagta agcagtaaaa tccccggcaa
     6481 tcgcctcacc cgtcactcct tgtctgctaa aaatgtgact caaaaaccct ttgccggatg
     6541 gcggcccagc atctgtttac atcattacga cgtcaaactg ctcctggtta tagagcggtt
     6601 caatttgtac tttaacctgt ttgccaacga aaatttccac ttccgccagc gagtgtgact
     6661 cttcgccttt caaggcttcg gctactgccg gagaagcata gaccaggaaa cggtcggagt
     6721 cgtaagcatg gtgtacacga acaatctcgc gcatgatttc atagcatacc gtttccacgg
     6781 ttttcaccgt tccgcgaccg tggcaggttg ggcattcgtt acacagtacg tgctcaatgc
     6841 tttcgcgggt gcgtttacgc gtcatctcca ccagccccag cgccgaaaaa ccattaacgc
     6901 tggttttcac ccggtctttg ctcaacgcct gctccagcga gtgcagcact cggcggcggt
     6961 gatcttcatt attcatatcg atgaaatcaa taatgataat cccgcccaga ttacgcaacc
     7021 gtaactggcg agcgatagcc tgcgtcgctt caatattggt attgaaaatg gtgtcgtcca
     7081 gattgcgatg accgacaaac gctccggtat tgatgtccac ggtggtcatc gcttcggtct
     7141 ggtcgataat cagataacca ccggatttca gttcaacttt gcgttccagc gctcgctgga
     7201 tttcgttttc gacatcaaag agatcgaaaa tcggctggcg tccggtgtaa tgctccagct
     7261 tgctggtcat ctcgggaatg tactccgagg tgaactcaag taacgcttcg taagtcaggc
     7321 gtgagtcaac gcgaatgcgg tccagttcgg catcggcgaa atcacgcaga acacgctgcg
     7381 ccaacgccag ttcgccgtac agctgataac gggtctgcgg gcgcttttta cgctccataa
     7441 ctttggtcca gacgcgtttc agataagcgg catcggaggc cagttcagcc tcgccaaccc
     7501 cttccgctgc ggtacggatg ataaacccgc cctgctcgtc gcaatactct gcgaccactt
     7561 ttttcaggcg ttcacgttct gattcgcttt caattcgttg ggaaacccca acgtgagaag
     7621 cccctggcat aaagaccaga tagcgagaag gcagcgtgat atcggtggtc aggcgcgccc
     7681 ccttagtgcc aagcggatct ttcaccacct gcaccatcag atcttgcccc tgacgaacca
     7741 gttccgagat gtcgcgcacc gtgaattgct tttgttcttc acccgccaca cattcggtgt
     7801 gcggcatgat gtcagatgca tgaagaaacg cggctttatc cagcccaata tctacaaaag
     7861 ccgcctgcat acccggaagt acacgactta cacgaccctt gtagatattg cctactattc
     7921 cgcgtcgcgc ctcacgttca atatgaattt cctgcagaat accgccatca atatacgcca
     7981 ctcgcgtttc cgaaggcgtt acgtttacta acaattcagc cgtcatgttt atcccttttc
     8041 tcacgcagtg cgttaaaatt acttaataat tcatacgttt caaccagcgg taagccgact
     8101 acggcttgat agctgccatt tatcttcctg acaaaacagc cacccagccc ctgaataccg
     8161 tatgcacctg ctttatctaa cggttcaccg ctggcgacat agcccgcgat gtcttcgtct
     8221 gttaacgttc tgaaagtcac atcggtgacc accaggcaat cgagaatgtg ccggctgtcg
     8281 gccaacgcca ctgccgtcat cacctgatgg gtctgacccg ataatttgcg caacatctgc
     8341 gccgcatgct ctgcgtcgcg cggtttctcc agcacttctc cgttcaggat aacgatagta
     8401 tccgcaccca gcaccgggag atccttcgcc gtttgcgcga cacctgcccg tgctttctcg
     8461 cgcgccagac gcacaacata ctgctgcgcg ctctcctgcg gctgacgctg ctcctcaatg
     8521 cccgtaacaa tacgttcaaa ggtcacgcca agttgcgcaa gtaactcctg acgacgcgga
     8581 gaaccggaag ctaaatacag agaagtcata gaaaccttta ttgcactgca aactgctgac
     8641 ggactttacg catcagcaag aaaatccacg gccagagcac cccattgact acactactcc
     8701 agaacacttc cggtctgaaa gagacgttaa tcactaaaaa ctctgcccag aaaacaataa
     8761 tatccaccac cagcgaaagc aacatgacga ccagcgcctg ctgccataat gcgaggttgc
     8821 ggaaaagctg gtatttcagc gccaccaggt aagcaatgat gctcatcgcc aatacgcgta
     8881 cgccaagcgt cgagccgctg atcagatcca gtatggcacc catcacaaaa cctgtgccca
     8941 catttacgcg atggggcaag gccaggatcc aatacaacaa gatgagtaac acccagtttg
     9001 gccggaaaac aatcaggtta tccggccagg gcatgatttg cagcaaaagc gcaatgagga
     9061 aagagagcca gattacccag cgtccctggc tacgatagct cgccactatt gccctcccgg
     9121 cgcacgcgca ggcggttgag caccactttg cggcggcgta gccctttgta gagagcgatt
     9181 agcagcgggc tgtgtgggcg cagcaggcgc agtagctgca tttcctgtcg tgggttgctg
     9241 cggagtcggc tgagcgatcc ccgttgccgg ttcaggtaac tttggcccca tcgcgtctgg
     9301 cgaaggcaat acctgcggca tcatctgcat cagacgttca ttagcaacac gatgcacctc
     9361 ttccggcgtc atcgggttag cgccgttacg atctgccccc cacagcagca gcagataacg
     9421 caaacgttgc agccctgcag tcggacgcgc ctgaatcaca gtataagcgc gctgggtatc
     9481 gagttttacg gaagagacaa ccgcgaccgg atagccttcc gggaaacgac cgcccagacc
     9541 ggaggtcacc agcacatcac caacacgaat atccgtattc gctggcagat gctcaagctg
     9601 caaatcatcc gtacaaccgt taccggctgc aattacgcgg atatcgttgc gcagcacctg
     9661 gattggcagc gcgtgggtcg catcacaaat cagcagcacg cgactggtca gtttagcgac
     9721 ggccaccacc tgaccaacaa cacctttgtc gctgatgacc ggctggcctt cataaacgcc
     9781 attaacgcta cctttatcga taacaacttg atcgctataa ggatcgttaa ccgtggagat
     9841 aacctgagtc accattttct gctcatcctg acgcagcggg gaacccagca gctcgcgcag
     9901 acgcgcgttc tcctgtttgt attgtccaag catcagcagt tcactgtttt tcagcaacag
     9961 ttcctgacgt aacgcccgat tttcaagttc tagttggtca cgcgaggcca gcgtctgcga
    10021 tacgccatcc agcaattcac gaggagcatt ggaaacaaag tagaaaggac tgacggcggt
    10081 atccatataa gtacggattt gactgaacgt ccccaggcgg ctgtcggcaa taataatgcc
    10141 gagcgccacc agcaccgcca gaataaggcg aatctgtagc gacgggccac ggctaaaaat
    10201 tggcttcata agttatgcgt attctcgtat cagaccaggc agggtaaaca gacgcttccc
    10261 ctgcctgcat ccgattactc ttcgctgaac aggtcgccgc cgtgcatgtc gatcatttcc
    10321 agcgctttgc cgccaccgcg cgccacacag gtcagcgggt cttcagcaac aacgactgga
    10381 atgccggttt cttccattaa caaacggtca aggttacgca gcagtgcgcc accaccggtg
    10441 agtaccatgc cgcgctcgga gatgtcggaa gccagttccg gcgggcactg ttccagtgca
    10501 accattaccg cgctcacaat accggtcagc ggttcctgca gtgcttcgag gatttcgttg
    10561 gagttcaggg taaaaccgcg tggaacacct tctgccaggt tacggccacg aacttcgatt
    10621 tcacggactt catcgcccgg ataagccgaa ccgatttcgt gcttgatacg ttctgcggtg
    10681 gcttcaccga tcagagaacc gtaattacga cgcacatagt tgatgatagc ttcgtcgaaa
    10741 cggtcaccac caatgcgtac ggaagaggag taaaccacgc cgttcaagga gataacagca
    10801 acttcagtgg taccaccacc gatatcaacc accatagaac cggtcgcttc agaaaccggc
    10861 aggccagcac caattgcggc agccatcggt tcttcaatca ggaagacttc acgggcacca
    10921 gcgccctgcg cggattcacg aattgcgcgg cgttcaacct gggtcgcgcc aaccggtaca
    10981 caaaccagaa cgcgcgggct tggacgcata aagctgttgc tgtgcacttg tttgatgaag
    11041 tgctggagca ttttttcagt cacgaagaag tcggcgataa cgccgtcttt cattgggcga
    11101 atggcagcaa tattgcccgg cgtacggccc agcatctgct tcgcgtcatg acctactgca
    11161 gctacgcttt tcggtgaacc ggcacgatcc tgacgaatgg ccaccacgga aggctcattc
    11221 aatacgatgc cttgtccttt tacataaatg agggtattcg cagtacccag gtcaatggac
    11281 aagtcattgg aaaacatgcc acgaaatttt ttcaacatac taagggataa tcctgaaagc
    11341 tggggcggaa aagaaaatcc gcttacttta ccaaccacac gcagcagcga caaggcgcaa
    11401 aaatcatctg ctacggtgaa aattagtgca gttcgtttcc tttgttacaa atctctgcct
    11461 gagtccagaa aggcttaatg catcagcagc attcctcgcc tgtttgcaac cgcgtaaggt
    11521 cattcatctg catatgtgct gcaacaatct ggcgagcaga caagcacact cccatgagac
    11581 gcagcgcgcg ttattctacg tgaaaacgga ttaaacggca ggttaaaccg agtatctttg
    11641 tgaatatttt ttcacgttag tatcaagtgg ctgtgaggac gcgaaaaaat ccccttgccc
    11701 gcctgtaaca ccgcgctgaa tcagggtctg ccactcgctt cgcgaacgca caccggtggc
    11761 gtaaacctgg gtgctggtcc ctgagcaggc ttccaccagg ctttgaacca gcagctggtt
    11821 ctccgttcgc ttctcaatgt ttctgaccag ccccggatgg agcttgagta actcaacatt
    11881 aagttctttg atccaactgg tgcttaccag cgtcaaacca gcctggttga cggctacccg
    11941 tacccctaaa gcattcacta aacgaataac aggttgtaaa cgactgatat gttgacctac
    12001 atcggcctct gcaagttcaa taattatgcg ttttcgttgt gatttttcac attgcattaa
    12061 cgtatcgcgc agccaacgct gaaaacgcgg gcgaatcagc gactcaacgg taacctgaat
    12121 cgccagattt tcctctggcc agtaacgcaa tagtggaata agacggctga tttgcagacg
    12181 gtcatactct tccgataagc caaactgcaa gaccatcggc atatactccg ccgagctaac
    12241 ctcttcatta ccatcaaaga tgcggcacat gagttcgcga tgatgaacct gaccttcgcg
    12301 agtaaccgcc ggtttttgat aaaggcgcgg gccgccgcga ctgagcattt gctcgataag
    12361 cgtacgccag cgaacattac cgcgtccttt ttcaggcaac gagtcatcgt aaatagccca
    12421 gctattgccg ccctgcaatc ccgcattacg cgtggcagac tctgcatgtt ccattacctg
    12481 ctcggtatcc tgaccactac gccaggcgca gataccaatg tggatcatat cgtcgcgatc
    12541 gagcatttta ttgttcggca aggtatcgac ggctttgatt aactgaccgg cgatgctctc
    12601 tgcctctttt aacgtccggt gcggtaacag cgcagcaaaa tcactgcggt ggtaacgcgc
    12661 cagcagtgcg ccagggtagc gcatcataaa tgtcgacagc agattcgtca gagtgaagaa
    12721 ctgttcttca acctggctgt gcccccaggt atcgctcaac atattgaaat ccggcagacg
    12781 aatcatcatc acgatcccgt gggtacctac tttctcctga tcttccagta acgttgctaa
    12841 ctgattatcg aaaaagagtc ggttattgag gccggttttc acgtcctgtg cggcataaga
    12901 gcggatcagc gtatcaagac ggctgtgttg ttcgcgtgcg ttctgaattt cacgaagcag
    12961 cgtatccagc gcactgctgg ttctgggcgg ccattcatag atggttccca acacattaga
    13021 gccacgctca ccgtttaaga tacgagtagc ccgggtttcc agcaattctt gcccggcaag
    13081 ttgccgttgt aaccagcgta ccgccagaaa gagcataaga atgataaagc caatcgcccc
    13141 tgtgagcggc gcggtggtca tcaacgaatg gaaatagttg cccatcggat cctgataaac
    13201 cagacgcaac gacatccccg gatgctttat caacggaacg ctcagttcac gaaacagatc
    13261 gctggtgcca accggacgat aactaccatt tctggccagg gtataaacct gcttatcacc
    13321 atggagcagg tctacacgaa cgatatctgc cgacatcatt aattcggtaa tttgtggcct
    13381 taatgcgctg aagtcattcg acacaaggtg ggtatcaatc gccgtcgcca ccgcctgaac
    13441 gcgatgacta aacttatact gaatggcgtt gtagaaactt agcgaacagc ccagcaaagt
    13501 cacaaaaatt gttaacccgg tgagcagcgt aacaaaggcc gaaaatttcg tcgttaatct
    13561 catccttgtg ttaactccga tagtgaggaa gcgggcatac tagcaaatca gatttatctc
    13621 gcaatttatt gcgcttcatc ggctttgctt ttccattagc gagtatagtc ttcagaaatt
    13681 attttccaat ccatcatgca catgaggacc acttatgcag gcgttacttt tagaacagca
    13741 ggacggcaaa actctcgcat cagtacagac tctggacgaa agtcgcctgc cggagggcga
    13801 tgtcacggtc gatgttcact ggtcgagcct gaactataaa gatgcgctgg cgattaccgg
    13861 taagggaaaa atcatccgta attttccgat gattcctggg atcgattttg ccggaactgt
    13921 acgcaccagc gaagatccgc gttttcatgc cggtcaggag gtgttactca ctggctgggg
    13981 cgttggtgaa aaccactggg gtgggctggc ggagcaggcg cgagtgaaag gtgactggct
    14041 ggttgccatg ccgcaagggc tggacgcgcg taaagcaatg attatcggta ctgccggttt
    14101 taccgccatg ctgtgtgtga tggcgctgga agatgccggt gttcgcccgc aggacgggga
    14161 gattgtcgtg acgggtgcca gtggtggcgt cggcagtacc gccgtggcgc tgctgcataa
    14221 gttgggttat caggtcgttg ccgtttccgg tcgcgaaagt acccatgaat atctgaaaag
    14281 tttaggtgcc agccgtattc tccctcgtga tgagtttgcc gaatcccgtc ctctggaaaa
    14341 acaagtctgg gctggggcaa ttgacactgt tggcgacaaa gtgctggcaa aagtgctggc
    14401 gcaaatgaat tacggcggct gcgtggcggc ctgtggtctg gcgggtggtt ttactctgcc
    14461 aaccacggtc atgccattta ttctgcgtaa tgtccgtttg caaggggtgg attcagtaat
    14521 gacgccacca gaacgccgcg cacaagcctg gcagcgactg gtcgccgatt taccggaatc
    14581 attctatacc caggcggcaa aagagatatc tctgtcagag gcaccgaact ttgccgaggc
    14641 catcattaat aaccagatcc agggtcgcac gctggtgaag gttaactaac catttagcaa
    14701 ggaataataa gagagggaac tcattttgaa atcattattc aatcggttaa cgggaaaagc
    14761 ggttagccgg acagctttcg tcgaacacct tggtcatgaa gttgtacaac atcatccaaa
    14821 ctggaaagtc atgatttcga ctgaccataa attgatgcgc attgatactc cactaaacag
    14881 ctattattga tacgcctccg tcgcctgtta ggtttatggt gctttgcctg ggcgacgcta
    14941 cgcttaaccc cttacttatt tctggtacca tgaggtgaat aatctgattt tgtttgacta
    15001 caaattaatc actcgaacct atttaatgct gagcattgtc aatcggttaa ttttgcgtgc
    15061 tttagcattc acatctatcc aggcgatgca gtgaaaattg ggtaatcccc agcaaccgct
    15121 gcgtaatgtc gtctatcttg tcgcgatcct ggcatcccta cattatttgt ggtctgtgac
    15181 gattatctca ttgcagcccc tcatcttcgc agggctggct ttacagcttt tcaccttacg
    15241 ttataagaag ttccgtcgat gatggcgcta atttcgtgaa ttgtgcggct tgttgcaaat
    15301 tacacggtgt tgaaggttat ttacatgtta gttgttgatt atcttccctg ataagaccag
    15361 tatttagctg ccaattgcta cgaaatcgtt ataatgtgcg acctcgtcct ccctgacgca
    15421 gtttttgcgc tgcggaaaag gtgacattgg cgcaacgaag gtatattttg ttttttgccg
    15481 gaggatagca gcagatcgct gcacaatgtc cgtcaagtct aacattgaca ctctggggca
    15541 aaatagaccg gcgtcccgac ctgctggaat ttatcgctat gcatacagct gtcggggcat
    15601 acgctttaca gacggcggtg aaacgcctgt cacaatcaca ctaaacaaag agtacggaac
    15661 ccactcatgg atattcgtaa gattaaaaaa ctgatcgagc tggttgaaga atcaggcatc
    15721 tccgaactgg aaatttctga aggcgaagag tcagtacgca ttagccgtgc agctcctgcc
    15781 gcaagtttcc ctgtgatgca acaagcttac gctgcaccaa tgatgcagca gccagctcaa
    15841 tctaacgcag ccgctccggc gaccgttcct tccatggaag cgccagcagc agcggaaatc
    15901 agtggtcaca tcgtacgttc cccgatggtt ggtactttct accgcacccc aagcccggat
    15961 gcaaaagcgt tcatcgaagt gggtcagaaa gtcaacgtgg gcgataccct gtgcatcgtt
    16021 gaagccatga aaatgatgaa ccagatcgaa gcggacaaat ccggtaccgt gaaagcaatt
    16081 ctggtcgaaa gtggacaacc ggtagaattt gacgagccgc tggtcgtcat cgagtaacga
    16141 ggcgaacatg ctggataaaa ttgttattgc caaccgcggc gagattgcat tgcgtattct
    16201 tcgtgcctgt aaagaactgg gcatcaagac tgtcgctgtg cactccagcg cggatcgcga
    16261 tctaaaacac gtattactgg cagatgaaac ggtctgtatt ggccctgctc cgtcagtaaa
    16321 aagttatctg aacatcccgg caatcatcag cgccgctgaa atcaccggcg cagtagcaat
    16381 ccatccgggt tacggcttcc tctccgagaa cgccaacttt gccgagcagg ttgaacgctc
    16441 cggctttatc ttcattggcc cgaaagcaga aaccattcgc ctgatgggcg acaaagtatc
    16501 cgcaatcgcg gcgatgaaaa aagcgggcgt cccttgcgta ccgggttctg acggcccgct
    16561 gggcgacgat atggataaaa accgtgccat tgctaaacgc attggttatc cggtgattat
    16621 caaagcctcc ggcggcggcg gtggtcgcgg tatgcgcgta gtgcgcggcg acgctgaact
    16681 ggcacaatcc atctccatga cccgtgcgga agcgaaagct gctttcagca acgatatggt
    16741 ttacatggag aaatacctgg aaaatcctcg ccacgtcgag attcaggtac tggctgacgg
    16801 tcagggcaac tctatctatc tggcggaacg tgactgctcc atgcagcgcc gccaccagaa
    16861 agtggtcgaa gaagcaccag caccgggcat taccccggaa ctgcgtcgct acatcggcga
    16921 acgttgcgct aaagcgtgtg ttgatatcgg ctatcgcggt gcaggtactt tcgagttcct
    16981 gttcgaaaac ggcgagttct atttcatcga aatgaacacc cgtattcagg tagaacaccc
    17041 ggttacagaa atgatcaccg gcgttgacct gatcaaagaa cagctgcgta tcgctgccgg
    17101 tcaaccgctg tcgatcaagc aagaagaagt tcacgttcgc ggccatgcgg tggaatgtcg
    17161 tatcaacgcc gaagatccga acaccttcct gccaagtccg ggcaaaatca cccgtttcca
    17221 cgcacctggc ggttttggcg tacgttggga gtctcatatc tacgcgggct acaccgtacc
    17281 gccgtactat gactcaatga tcggtaagct gatttgctac ggtgaaaacc gtgacgtggc
    17341 gattgcccgc atgaagaatg cgctgcagga gctgatcatc gacggtatca aaaccaacgt
    17401 tgatctgcag atccgcatca tgaatgacga gaacttccag catggtggca ctaacatcca
    17461 ctatctggag aaaaaactcg gtcttcagga aaaataagac tgcaaaagcg tcaaaaggcc
    17521 ggattttccg gcctttttta ttactgggga tcgacaacac ccataaggta caatcctcgc
    17581 tttcttcacc catcagggac aaaaaatgga cactcgtttt gttcaggccc ataaagaggc
    17641 gcgctgggcg ctggggctga cccttttgta tctggcagtt tggttagtag ccgcttactt
    17701 acctggcgtt gcccccggtt ttaccggctt tccgcgctgg tttgagatgg cctgcatcct
    17761 gacgccgctg ctgtttattg gactgtgctg ggcgatggtg aaatttatct atcgcgatat
    17821 cccgctggag gatgacgatg cagcttgaag taattctacc gctggtcgcc tatctggtgg
    17881 tggtgttcgg tatctcggtt tatgcgatgc gtaaacggag caccggcacc ttccttaatg
    17941 agtatttcct cggcagccgc tctatgggcg gtattgtgct ggcgatgacg ctcaccgcga
    18001 cttatatcag tgccagttcg tttatcggcg ggcctggagc tgcttacaaa tacggccttg
    18061 gctgggtatt gctggcgatg attcagcttc cggcagtctg gctttcactc ggtattctcg
    18121 gcaagaagtt tgcgattctt gcgcgccgct acaatgcagt gacgctgaac gatatgctgt
    18181 ttgcccgcta ccagagtcgt cttctggtgt ggctggcgag tttgagtttg ctggttgcgt
    18241 tcgttggtgc gatgaccgtg cagtttatcg gcggtgcgcg cctgctggaa accgcggcgg
    18301 gtattcctta tgaaaccggg ctgctgattt ttggtatcag cattgcgtta tataccgcct
    18361 ttggtggctt tcgcgccagc gtgctgaacg acaccatgca agggcttgtg atgctgattg
    18421 gcaccgttgt gctgcttatt ggcgtggtac atgccgctgg cggcttaagc aacgcagtac
    18481 agaccttgca aaccatcgat ccgcaactgg ttacgccaca aggcgctgac gatattctgt
    18541 cgcctgcctt tatgacgtcg ttctgggtac tggtgtgctt tggcgttata ggcctgccgc
    18601 atactgcggt gcgctgtatc tcttataaag acagcaaagc agtacaccgg gggatcatca
    18661 tcggtacgat tgtagtcgca atcctgatgt tcggtatgca cctagccgga gcgttaggtc
    18721 gtgcagtgat ccccgatctc accgtaccgg atctggtgat cccgacgtta atggtaaaag
    18781 tgctgccacc gtttgctgcc gggatctttc tggctgcacc tatggctgcg atcatgtcga
    18841 caattaacgc ccaattgcta caaagttccg ctacgatcat taaagatctc tatctgaata
    18901 tccgtccgga tcaaatgcaa aacgagacgc gtctgaagcg gatgtcggcg gtaattacgt
    18961 tagttctcgg cgcgttgctg ctgcttgccg cctggaagcc gccagaaatg atcatctggc
    19021 tgaatttgct ggccttcggt gggctggaag ccgttttcct gtggccgctg gtgctgggtc
    19081 tttactggga acgcgccaac gccaaaggcg cgctaagtgc gatgatcgtt ggcggcgtgc
    19141 tgtatgccgt actcgcgacg ctgaatattc agtacctggg cttccaccct atcgtgccct
    19201 cgttactact aagtttgctg gctttcctgg tcggaaaccg tttcggtaca tccgtcccgc
    19261 aagctaccgt tttgactact gataaataaa gagttttgcc atgccttgga tccaactgaa
    19321 actgaacacc accggcgcga acgcggaaga tcttagcgat gcgctgatgg aagcgggtgc
    19381 cgtttctatc acttttcagg atacccacga tacgccagta tttgagccgc tgccgggcga
    19441 aacgcgcctg tggggcgaca ccgatgtgat tggtctgttc gacgctgaaa ccgatatgaa
    19501 cgacgtggtg gcgattctgg aaaaccatcc gctgctcggc gcaggcttcg cgcataaaat
    19561 cgaacaacta gaagataaag actgggagcg cgaatggatg gataatttcc acccgatgcg
    19621 ctttggcgaa cgactgtgga tctgccctag ctggcgtgat gtgccggacg aaaacgccgt
    19681 caacgtgatg ttagatccag ggctggcgtt tggtacgggt acccatccaa ccacctctct
    19741 gtgcctgcaa tggctcgaca gcctcgattt aaccggtaaa acagtcatcg actttggctg
    19801 tggttccggc attctggcga tcgcggcgct gaaactgggt gcagcaaaag ccattggtat
    19861 tgatatcgat ccgcaggcga ttcaggccag ccgcgataac gccgaacgta atggcgtttc
    19921 tgaccgtctg gaactctact taccgaaaga tcagccagaa gaaatgaaag ccgacgtggt
    19981 ggtcgctaac atccttgcag gcccattacg tgaactggca ccgttaatta gcgtcctgcc
    20041 agtttcaggc ggcttactgg gcctttctgg tattctggca agccaggcgg agagcgtttg
    20101 cgaagcctat gccgacagct tcgctctgga cccggtcgtg gaaaaagaag agtggtgccg
    20161 tattaccggt cgtaagaatt aaccttcgca tcgccatagg gtgacgcggg gcgaagtgcg
    20221 agcaagctca caaaaggcac gtaaatttgc cgattattta cgcaaatttg cgtgccaaaa
    20281 ttttcattca taaagaaaaa ttgagaactt actcaaattt ctttgagtgt aaattttagt
    20341 cactattttc taatatgatg atttttatga gtaattatcg caccacgctc attttaaatg
    20401 caattctttg atccatctca gaggattggt caaagtttgg cctttcatct cgtgcaaaaa
    20461 atgcgtaata tacgccgcct tgcagtcaca gtatggtcat ttcttaactc atgcgcatcg
    20521 gacaatatca gctcagaaat cgcctgatcg cagcgcccat ggctggcatt acagacagac
    20581 cttttcggac gttgtgctac gagatgggag ccggattgac agtatccgag atgatgtctt
    20641 ctaacccaca ggtttgggaa agcgacaaat ctcgtttacg gatggtgcac attgatgaac
    20701 ccggtattcg caccgtgcaa attgctggta gcgatccgaa agaaatggca gatgcagcac
    20761 gtattaacgt ggaaagcggt gcccagatta ttgatatcaa tatgggttgc ccggctaaaa
    20821 aagtgaatcg caagctcgca ggttcagccc tcttgcagta cccggatgtc gttaaatcga
    20881 tccttaccga ggtcgtcaat gcagtggacg ttcctgttac cctgaagatt cgcaccggct
    20941 gggcaccgga acaccgtaac tgcgaagaga ttgcccaact ggctgaagac tgtggcattc
    21001 aggctctgac cattcatggc cgtacacgcg cctgtttgtt caatggagaa gctgagtacg
    21061 acagtattcg ggcagttaag cagaaagttt ccattccggt tatcgcgaat ggcgacatta
    21121 ctgacccgct taaagccaga gctgtgctcg actatacagg ggcggatgcc ctgatgatag
    21181 gccgcgcagc tcagggaaga ccctggatct ttcgggaaat ccagcattat ctggacactg
    21241 gggagttgct gcccccgctg cctttggcag aggttaagcg cttgctttgc gcgcacgttc
    21301 gggaactgca tgacttttat ggtccggcaa aagggtaccg aattgcacgt aaacacgttt
    21361 cctggtatct ccaggaacac gctccaaatg accagtttcg gcgcacattc aacgccattg
    21421 aggatgccag cgaacagctg gaggcgttgg aggcatactt cgaaaatttt gcgtaaacag
    21481 aaataaagag ctgacagaac tatgttcgaa caacgcgtaa attctgacgt actgaccgtt
    21541 tctaccgtta actctcagga tcaggtaacc caaaaacccc tgcgtgactc ggttaaacag
    21601 gcactgaaga actattttgc tcaactgaat ggtcaggatg tgaatgacct ctatgagctg
    21661 gtactggctg aagtagaaca gcccctgttg gacatggtga tgcaatacac ccgtggtaac
    21721 cagacccgtg ctgcgctgat gatgggcatc aaccgtggta cgctgcgtaa aaaattgaaa
    21781 aaatacggca tgaactaatt caggttagct aattgcttga ttaaaaggcg ctactcggca
    21841 tggggaagcg ccttttttat aggtgtcaca aagggagtga ccatgagaac aggatgtgaa
    21901 ccgacccggt ttggtaatga agctaagacc attattcacg gtgatgccct tgccgaactt
    21961 aaaaagctac ctactgaaag cgtcgatctg atctttgccg acccaccgta taacatcggt
    22021 aaaaattttg atggtctgat cgaagcctgg aaagaagatc tgtttatcga ctggctgtta
    22081 gaagtgattg cagagtgcca ccgcgttctg aaaaagcagg gcagcatgta catcatgaac
    22141 agtacggaaa acatgccctt tatcgatctc cagtgccgca agctttttac catcaaaagt
    22201 cgcatcgtct ggtcatatga cagttctgga gtgcaggcga aaaaacacta cggctccatg
    22261 tacgaaccca tcctgatgat ggtgaaagac gcaaagaact acacattcaa cggtgatgct
    22321 attctggtag aagccaaaac cggatcgcag cgcgcgttga tcgattatcg caaaaatcct
    22381 ccacagcctt acaatcatca aaaagtaccg ggtaacgtct gggattttcc gcgcgtgcgt
    22441 tatttgatgg atgaatatga aaaccacccg acgcaaaaac cggaagcctt actgaaacgc
    22501 attattctcg cctcttccaa cccaggcgat atcgttctcg acccgtttgc tggtagcttt
    22561 actaccggtg ccgtagccat cgccagcgga cgaaaattca ttggtattga gatcaacagc
    22621 gagtacatca aaatggggct tcgacggctg gatgtcgcgt cgcattactc tgcggaagaa
    22681 ctggcgaaag tgaaaaaaag aaagaccggc aacctgtcaa aacgaagccg gttaagcgaa
    22741 gttgaccccg atctcattac aaagtaaagg atgtgtaagc ctggtttgca gattattcat
    22801 ttcgtgtata tttcctgcca gacttggtta aacatgcaca ggctcaggta atgattcgca
    22861 agtattggtg gctcgtcgtt ttcgctgtct tcgtttttct gtttgatact ttactgatgc
    22921 agtggattga actgctggca acagaaacag acaaatgccg caatatgaac tcagttaatc
    22981 cactaaaact ggttaactgt gacgaactga attttcagga cagaatgtga atttacatga
    23041 cacttaattc attcgtttga tgaattaatt tcgttatgtt ttcatctggc atgaacattc
    23101 ttaatacgtt atcgaccaga gcgggggctt gtttataaag atcataaccc gccatattca
    23161 ttaaccagtt ttgaacaatt ccgctgaagg caccatcaat aataatcatc acaacatcta
    23221 aatcgaggtt atttgctaca caaccttgtt gctgacacgc ctgcaatact tcgcggagag
    23281 tctgcggatt aaagcccatc ttttcgcgta tcactccctc ggccagcatc tcatcattaa
    23341 attcacattt gtgatataag attttcagca acgcctgctg gcggggaatt ttggcaatat
    23401 attgcaagcc gacaatcaat ttttcacgca attgttgaaa cgggtcatgc tctaatccag
    23461 ccgtcaagtg ttcctggatt aactcccgca atgaaggctg ttgcaaccac atctcattaa
    23521 acagttgggt cttgttctcg aagtgccagt agatagcgcc acgcgtaacg ttagcggcat
    23581 cggcaatgtc gttgagcgtc gtcttgctta cgccatgttg cgcaaactgg gcgatggcag
    23641 tttcaatcag ttcttgccgg gtcttcagag cttcggcttt ggttcttttt gccatgatta
    23701 attattcagg aaataaatat cttcgacaca gagtgagaaa atagcgaagg ttaatctatc
    23761 acctaatgtg tatttatacg agaggctaac attgagttgc
//
LOCUS       DAZKIP010000067        23371 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZKIP010000067 DAZKIP010000000
VERSION     DAZKIP010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.067"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            405..685
                     /gene="drpB"
                     /locus_tag="ACHOUK_002861"
                     /pseudo
     CDS             405..685
                     /gene="drpB"
                     /locus_tag="ACHOUK_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
     gene            725..1420
                     /gene="yedJ"
                     /locus_tag="ACHOUK_002862"
     CDS             725..1420
                     /gene="yedJ"
                     /locus_tag="ACHOUK_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFP9898271.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQSSSILAAEETRRLLREEFVQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDAEDPLAQ
                     HRPLDDKRYALDHFQTKLLKLPQIMQTVRGKQLAQHNAHFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            1487..2905
                     /gene="dcm"
                     /locus_tag="ACHOUK_002863"
     CDS             1487..2905
                     /gene="dcm"
                     /locus_tag="ACHOUK_002863"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFP9898272.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            2886..3356
                     /gene="vsr"
                     /locus_tag="ACHOUK_002864"
     CDS             2886..3356
                     /gene="vsr"
                     /locus_tag="ACHOUK_002864"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFP9898273.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLKDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(3345..4265)
                     /gene="yedA"
                     /locus_tag="ACHOUK_002865"
     CDS             complement(3345..4265)
                     /gene="yedA"
                     /locus_tag="ACHOUK_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFP9898274.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            4438..5355
                     /gene="yedI"
                     /locus_tag="ACHOUK_002866"
     CDS             4438..5355
                     /gene="yedI"
                     /locus_tag="ACHOUK_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFP9898275.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQVH"
     gene            5434..5616
                     /gene="yodC"
                     /locus_tag="ACHOUK_002867"
     CDS             5434..5616
                     /gene="yodC"
                     /locus_tag="ACHOUK_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFP9898276.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            5787..7481
                     /gene="dgcQ"
                     /locus_tag="ACHOUK_002868"
     CDS             5787..7481
                     /gene="dgcQ"
                     /locus_tag="ACHOUK_002868"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFP9898277.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHIVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRDAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(7478..8293)
                     /gene="yedP"
                     /locus_tag="ACHOUK_002869"
     CDS             complement(7478..8293)
                     /gene="yedP"
                     /locus_tag="ACHOUK_002869"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFP9898278.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKMLGLQGLPLIAENGAVIQLAEQWQEIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(8591..8818)
                     /gene="yodD"
                     /locus_tag="ACHOUK_002870"
     CDS             complement(8591..8818)
                     /gene="yodD"
                     /locus_tag="ACHOUK_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFP9898279.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            8981..9169
                     /gene="dsrB"
                     /locus_tag="ACHOUK_002871"
     CDS             8981..9169
                     /gene="dsrB"
                     /locus_tag="ACHOUK_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFP9898280.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            complement(9213..9836)
                     /gene="rcsA"
                     /locus_tag="ACHOUK_002872"
     CDS             complement(9213..9836)
                     /gene="rcsA"
                     /locus_tag="ACHOUK_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFP9898281.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKLIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(10126..10911)
                     /gene="fliR"
                     /locus_tag="ACHOUK_002873"
     CDS             complement(10126..10911)
                     /gene="fliR"
                     /locus_tag="ACHOUK_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFP9898282.1"
                     /translation="MLQVTSEQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            complement(10919..11188)
                     /gene="fliQ"
                     /locus_tag="ACHOUK_002874"
     CDS             complement(10919..11188)
                     /gene="fliQ"
                     /locus_tag="ACHOUK_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFP9898283.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            complement(11198..11935)
                     /gene="fliP"
                     /locus_tag="ACHOUK_002875"
     CDS             complement(11198..11935)
                     /gene="fliP"
                     /locus_tag="ACHOUK_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFP9898284.1"
                     /translation="MRRLLSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            complement(11935..12300)
                     /gene="fliO"
                     /locus_tag="ACHOUK_002876"
     CDS             complement(11935..12300)
                     /gene="fliO"
                     /locus_tag="ACHOUK_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFP9898285.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            complement(12303..12716)
                     /gene="fliN"
                     /locus_tag="ACHOUK_002877"
     CDS             complement(12303..12716)
                     /gene="fliN"
                     /locus_tag="ACHOUK_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFP9898286.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            complement(12713..13717)
                     /gene="fliM"
                     /locus_tag="ACHOUK_002878"
     CDS             complement(12713..13717)
                     /gene="fliM"
                     /locus_tag="ACHOUK_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFP9898287.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            complement(13722..14186)
                     /gene="fliL"
                     /locus_tag="ACHOUK_002879"
     CDS             complement(13722..14186)
                     /gene="fliL"
                     /locus_tag="ACHOUK_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFP9898288.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            complement(14291..15418)
                     /gene="fliK"
                     /locus_tag="ACHOUK_002880"
     CDS             complement(14291..15418)
                     /gene="fliK"
                     /locus_tag="ACHOUK_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFP9898289.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLISDIVSDAQQANLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMALAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPQVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSSHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            complement(15415..15858)
                     /gene="fliJ"
                     /locus_tag="ACHOUK_002881"
     CDS             complement(15415..15858)
                     /gene="fliJ"
                     /locus_tag="ACHOUK_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFP9898290.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            complement(15877..17250)
                     /gene="fliI"
                     /locus_tag="ACHOUK_002882"
     CDS             complement(15877..17250)
                     /gene="fliI"
                     /locus_tag="ACHOUK_002882"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFP9898291.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            complement(17312..17998)
                     /gene="fliH"
                     /locus_tag="ACHOUK_002883"
     CDS             complement(17312..17998)
                     /gene="fliH"
                     /locus_tag="ACHOUK_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFP9898292.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            complement(17991..18986)
                     /gene="fliG"
                     /locus_tag="ACHOUK_002884"
     CDS             complement(17991..18986)
                     /gene="fliG"
                     /locus_tag="ACHOUK_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFP9898293.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            complement(18979..20636)
                     /gene="fliF"
                     /locus_tag="ACHOUK_002885"
                     /pseudo
     CDS             complement(18979..20636)
                     /gene="fliF"
                     /locus_tag="ACHOUK_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310704.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
     gene            20851..21165
                     /gene="fliE"
                     /locus_tag="ACHOUK_002886"
     CDS             20851..21165
                     /gene="fliE"
                     /locus_tag="ACHOUK_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFP9898294.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            complement(21190..21330)
                     /locus_tag="ACHOUK_002887"
                     /pseudo
     CDS             complement(21190..21330)
                     /locus_tag="ACHOUK_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003844207.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            21514..22653
                     /gene="espR4"
                     /locus_tag="ACHOUK_002888"
     CDS             21514..22653
                     /gene="espR4"
                     /locus_tag="ACHOUK_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector leucine-rich repeat protein EspR4"
                     /protein_id="HFP9898295.1"
                     /translation="MFPLNDLSLKTQSVQLNKITSNTESTIKQHELVSDDAIINELSS
                     ELVSCLGNDKFTPVSEDCNLLNMLSEFKLLREQCFRWGNYTLLFENYEAYDKTGSITI
                     EKNQGEGTLPIRHKLEFISTNIAELLDKLTKITDARLCKGFSDWASSVKEGGSNDLKE
                     NVDRALVRMFKCVKLHSNELDLSYLFLGSVPPLPDWIEMLSLVYNELDSIQVPESCKE
                     LELDFNNLTEFPQVPDGITLISVNNNLISHIDSFPPKAKKIFISHNKLSETPAIPDTA
                     KVFDCGYNKIQEIRYFPKNLKEARIGYNNIEVVPAIPGNLKILFMECNPIKEAFLMPW
                     TLTGICYEISQRKYIVTNPADYDKYSDMVKKHVIDGEELIIKYFM"
     gene            complement(22791..23120)
                     /locus_tag="ACHOUK_002889"
                     /pseudo
     CDS             complement(22791..23120)
                     /locus_tag="ACHOUK_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416442.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(23078..23288)
                     /locus_tag="ACHOUK_002890"
                     /pseudo
     CDS             complement(23078..23288)
                     /locus_tag="ACHOUK_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140296.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         5686 a         5865 c         6081 g         5739 t
ORIGIN      
        1 tggggggtat caatgattgt tgcgctaaac ggctggtgtg atcacgcgca cattaatgaa
       61 ggcataaaaa aaagccggca tcatgccggc ccaaaaaata taaacaagac gataaaaggc
      121 taataacgga agcatcatga cacagaataa taaaaaggta taattgtaat cagtgctaat
      181 atattgattg aatgaattaa taaagccgat ctcaaagata aataaaatcg ctcaaaacga
      241 tttttcctgt attcactgcc gttgcgcaaa aattatctat ttgttcaaaa aatgagtgag
      301 tcttgactgg ctcatccaat gtggaaaaat gtgactttta tcacgtaata gtactaagtc
      361 tgaattttcc gggttatctc aaaatggaat acggttcgac aaagatggaa gagagactct
      421 ctcgcagccc tggcggcaaa ctggctttgt gggcttttta tacatggtgt ggctatttcg
      481 tctgggcaat ggcgcgttac atctgggtga tgagccggat ccccgatgcc cccgtgtcgg
      541 gatttgaaag cgatttaggc tctaccgcgg gaaagtggtt agggcactgg tcggattttt
      601 atttatggct ctggtcgggg cattgttagg cagcattgcc tggtacacac gacctcgtcc
      661 cgcacgtagc agacgctatg aataaaaact gctaaggtgg cgactctttg gcaacggagc
      721 gcgtatggac ttacaacact ggcaggcaca gttcgaaaac tggttgaaaa accatcatca
      781 gcaccaggac gcggcccatg acgtgtgtca ttttcgccgc gtctgggcta cggcacagaa
      841 gctggcggca gatgatgacg tcgatatgct ggtgatttta accgcctgtt atttccacga
      901 tattgtgagc ctggcgaaaa atcatccgca acggcagagt tcttcaatcc tggcggcaga
      961 agagacgcgt cgcctgctcc gtgaagagtt cgtgcaattt ccggcggaga aaatcgaggc
     1021 cgtttgtcat gccattgccg ctcacagttt cagcgcgcaa atcgccccct taacaacgga
     1081 agctaaaatc gtccaggatg cggatcggct ggaagccctg ggggcgattg gcctggcgcg
     1141 cgtgtttgcc gtttcagggg cattgggcgt ggcgctgttt gatgccgaag atccgctcgc
     1201 acagcatcgc ccgcttgatg ataaacgcta cgcgctggat catttccaga ctaagttgtt
     1261 gaagctaccg caaatcatgc aaaccgtaag aggcaagcag ctggcgcagc acaacgcgca
     1321 ttttttagtc gagtttatgg cgaagctcag tgccgaactg gcgggggaga atgaaggtgt
     1381 cgatcacaag gtgatagatg cgttttcacc cgcgggctga gcgcgtggcc ctaaatggct
     1441 gtaattatgt taacctgtcg accatctcag atggccggtt aaatctatgc aggaaaatat
     1501 atcagtaacc gattcataca gcaccgggaa tgccgcacag gcaatgctgg agaaactgct
     1561 gcaaatttat gatgttaaaa cgctggtggc tcagcttaat ggtgtgggtg agaatcactg
     1621 gagcgcggca attttaaaac gtgcgctggc gaatgactcg gcatggcacc gtttaagtga
     1681 gaaagagttc gcccatctgc aaacgttgtt acccaaacca ccggcacatc atccgcatta
     1741 tgcgtttcgc tttatcgatc tatttgccgg aattggcggc atccgtcgcg gttttgaatc
     1801 gattggcgga cagtgcgtgt ttaccagcga atggaacaaa catgcggtac gcacttataa
     1861 agccaatcat tattgcgacc cggcgacgca tcattttaat gaagatatcc gcgacatcac
     1921 cctcagccat aaagaaggcg tgagtgatga ggctgcggcg gaacatattc gtcaacacat
     1981 tcctgaacac gatgttttac tggccggttt cccttgtcag ccattttcgc tggctggcgt
     2041 atcgaaaaag aactcgctcg ggcgggcgca cggttttgcc tgcgatactc agggcacgct
     2101 gttctttgat gtggtgcgca ttatcgacgc gcgtcgtccg gcgatgtttg tgctcgaaaa
     2161 cgtcaaaaac ctgaaaagcc acgaccaggg taaaacgttc cgcatcatca tgcagacgct
     2221 ggacgaactg ggctatgacg tggctgatgc agaagataac gggccggacg atccgaaaat
     2281 catcgatggc aaacattttc tgccgcagca ccgtgaacgc atcgtgctgg tgggttttcg
     2341 tcgcgatctt aatctgaaag ccgattttac tctgcgtgat atcagcgaat gtttccctgc
     2401 acagcgagtg acgctggcgc agctgttgga cccgatggtc gaggcgaaat atatcctgac
     2461 gccggtgctg tggaagtacc tctatcgata tgcgaaaaaa catcaggcgc gcggaaacgg
     2521 cttcggttat ggaatggttt atccgaacaa tccgcaaagc gtcacccgta cgctgtctgc
     2581 gcgttattac aaagatggcg cggaaatttt aatcgatcgc ggctgggata tggccacggg
     2641 tgagaaagac tttgacgatc cgctgaatca gcaacatcgt ccacgtcggt taacgcctcg
     2701 ggaatgcgcg cgcttaatgg gttttgaagc gccgggagaa gcgaaattcc gcattccggt
     2761 ttcggacact caggcctatc gccagttcgg taactcggtg gtcgtgccgg tctttgccgc
     2821 ggtggcaaaa ctgcttgagc caaaaatcaa acaggcggtg gcgttgcgtc agcaagaggc
     2881 acaacatggc cgacgttcac gataaggcca ctcgcagcaa aaatatgcgc gcgattgcca
     2941 cgcgtgatac ggcgattgag aagcgcctcg ccagtctgtt aaccgggcag ggcctggcgt
     3001 ttcgcgttca ggacgccagt ctgcccggac gcccggattt tgtcgttgat gaatatcgct
     3061 gcgtgatatt tacccatggc tgcttctggc atcatcatca ctgctatctg tttaaagtgc
     3121 ctgcgactcg aaccgagttc tggctggaga agataggtaa aaatgttgag cgcgatcgcc
     3181 gcgatatcag tcgcttgcag gaactcggct ggcgcgtatt gattgtctgg gagtgcgcgt
     3241 tacgtgggcg cgagaagctg aaggatgaag cgcttaccga gcgtctggaa gagtggatct
     3301 gcggcgaagg agccagcgcg cagatcgaca cgcaggggat acatttactc gcttgatgcg
     3361 tcctgaataa ctggagcaac tacgggtttt gccggaaaga gatattttcc caacgtgacc
     3421 agtaccaccg cgaagacaat tacgccgagc gccagccatt caatcttcga cagtgtttct
     3481 ccacccagtc ccgtacccag caagaccgcg accaccgggt taacgtaagc gtagctggtg
     3541 gcgagagccg gactgacatt acggattaaa tacatataag cgttgatggc gataatcgaa
     3601 ccaaacagcg ccagatagcc gaccgcaagg aagcctgaaa gggaagggag cgccgtcagt
     3661 ttttcacccg caatcatcga cgcgatcatt aacaccacgc ctgccgccag catctcaatc
     3721 gcacccgcca tcatccctac aggtaaggta atgcgcgagc cataaactga gccaaacgcc
     3781 cagctaatcg agccgattaa aatcagaatc gcgccccacg gattgccgct taaatttcca
     3841 ccgctattga gcatgatgat tccggcaagc ccaatggcaa tacccaccca ttccagtttg
     3901 cgcgttttaa tgccaaacag gcggctgaag cacagggtaa agaggggcac ggttgcaacc
     3961 actacggcgg cgatgccgga aggaacattt tgatgttcgg caaccgtcac catgccatta
     4021 ccgacagcca gcaataacag gccaatcagc gcggcattga gcagcggacg tagcgggggg
     4081 agtttgtgtc cgcgcagtag taaaaatgcc agcaataaaa taccggctgc caggaatcga
     4141 acacccgcca tcattaacgg aggccagctt tccacgccaa tccgaatgac aaaataggtt
     4201 gagccccaaa tgatatacaa cgcaaacagc gcgccaaaaa gcggtaacaa ctggcggaaa
     4261 cgcataatcc ctcacggtgg aaataaaaag gtggttcata gtaaacgtga aaatcattct
     4321 gctggcgaga gatataattg cacttgattg ttaaaaaaat gttgacctgt gaagcaagta
     4381 tcagagctgc cgtttttgct tcatacttac acccttcaac aataaaaatg agagggaatg
     4441 cttttggccg ggagtagttt actgacgttg ctcgatgata tcgccacact gctggacgat
     4501 atctccgtga tgggcaaact ggcggcgaag aaaaccgccg gtgtattagg ggatgactta
     4561 tcgctcaatg cgcaacaagt ttcaggcgtg cgggccaacc gggaacttcc cgtggtctgg
     4621 ggcgtggcga aaggatcgct gattaataaa gtgattctgg tgccactggc gctgatcatc
     4681 agtgcgttta tcccgtgggc gattacgcca ttgttgatga tcggtggcgc gtttctctgc
     4741 tttgaagggg tagagaaagt gctgcatatg ctggaggcgc gtaaacataa agaagatccg
     4801 gcgcagagcc agcagcgtct ggagaagctg gcggcgcagg atccgctgaa gtttgaaaag
     4861 gacaaaataa aaggggcgat tcgtaccgat tttatattgt ctgcggaaat cgtcgccatc
     4921 acgctgggga ttgtggccga agcgccgttg cttaatcagg tgctggtgct ttcaggcatc
     4981 gcgctggtag tgaccgtggg cgtctacggt ctggtagggg ttatcgttaa gattgatgac
     5041 ctggggtatt ggctggcgga aaaatccagc gcgctgatgc aggcattagg taaaggatta
     5101 ttgattatcg cgccctggct gatgaaagcg ttatcgattg tcggcacgct ggcgatgttc
     5161 ctcgtcggcg gcgggattgt ggtacatggt attgcgccgc tgcatcacgc cattgaacat
     5221 ttcgccgggc agcaaagtgc agtggtggcg atgatattac cgactgtttt aaatctgatt
     5281 cttggattta tcatcggcgg catcgtggtg ctgggagtga aagccgtagc gaaaatgcgc
     5341 ggtcaggtac attaatactc gcacaggcta ttatgcaaaa ttcgccatct tcaactaagg
     5401 tgaacaagtt tcactcgaaa aaaggaggcg agtatgagct ttatggttag tgaggaagtt
     5461 acggttaaag agggcggccc gcggatgatt gtcactggat actccagcgg tatggttgag
     5521 tgtcgttggt atgacggtta cggggtcaag cgggaagctt ttcatgaaac cgagcttgtt
     5581 ccaggggagg ggagtcgttc tgcggaagaa gtttgaaagg gtatcattaa gaccaggccg
     5641 gatcacatcc ggcctggtgt gccaatgcct gcaatgaatg atagtcgcgt cttatcatgc
     5701 caccattata aaattatcgc tgttttgcgg tacgctatcg ccaggttatg ccagaatcat
     5761 aaaaaagcag gtggggagtc gtcagggtgc agcacgagac aaaaatggaa aaccagagct
     5821 ggttgaaaaa actcgcacgc cgcctagggc ctggtcatat cgttaatctc tgctttatcg
     5881 tggtattgct tttttccacc ttgctgacct ggcgtgaagt agtggtgctg gaagatgcct
     5941 atatctccag ccagcgtaat catctggaaa acgtagccaa cgcgctcgat aagcatttgc
     6001 agtataacgt cgacaaactg atctttttgc gtaatggcat gcgcgaagct ctcgtagcgc
     6061 cactggattt cacctctctg cgtgatgctg taaccgagtt cgaacagcat cgcgacgagc
     6121 acgcctggca aatcgaactc aaccgacgac gcaccctgtc agtcaatggc gtatcggatg
     6181 cattagtcag cgaggggaat ctcctgtctc gcgaaaatga aagcctcgac aatgaaatta
     6241 ccgctgcact ggaagttggt tacttgctgc gactggcaca caactcctcg tcgatggtag
     6301 aacaggcgat gtatgtctcg cgtgccggat tttacgtttc gacgcagccg accttgttta
     6361 cgcgcaatgt cccgacgcgt tattacggct atgtcaccca accctggttt atcggccatt
     6421 cacaacgaga aaatcgtcac cgcgcggtac gctggttcac ttcgcaaccg gaacacgcca
     6481 gcaatactga accgcaggtt accgtcagtg ttccggtaga cagtaataac tactggtatg
     6541 gcgtgctggg gatgagtatt cccgtgcgta ctatgcagca atttttaaga aacgccatcg
     6601 ataaaaacct cgatggtgag tatcagctct atgacagtaa gctgagattt ttgacctctt
     6661 ccaatcctga ccatccaaca gggaatattt ttgatcctcg tgaactggcc ttgctggcgc
     6721 aggcaatgga acatgacacg cggggcggca ttcgtatgga cagtcgctat gttagctggg
     6781 aacgtctgga ccatttcgac ggtgtgctgg tgcgcgtcca tacgttaagc gaaggcgtgc
     6841 gcggcgattt cggcagtatc agcattgcat taaccctgct gtgggcgctc tttaccacca
     6901 tgttactcat ctcctggtat gtgattcgcc ggatggtcag caacatgtat gttctgcaaa
     6961 gctcgttgca gtggcaggcg tggcacgaca ccttaacgcg tttatataac cgtggcgcac
     7021 tgttcgaaaa agcccgtccg ctcgcgaaat tgtgtcagac gcaccaacat cctttttctg
     7081 tcattcaggt cgatcttgac cattttaaag cgattaatga ccgctttggt catcaggcgg
     7141 gcgaccgtgt tctttctcat gctgccggat taattagcag ttccttgcgt gcgcaggacg
     7201 ttgccgggcg ggtcggtggt gaggagtttt gtgtgattct gccaggcgcg agtctgacgg
     7261 aggctgcgga agtcgcagaa cgtattcgcc tgaagttaaa tgaaaaagag atgttgattg
     7321 ctaagagtac gacgatacgc atcagtgcct cgttgggggt aagtagcagc gaggaaaccg
     7381 gtgattatga ttttgaacaa ctccagtcac tggccgaccg tcggctttat ctcgctaaac
     7441 aggctgggcg caatcgggta ttcgcgagcg ataacgctta acgggcggag aaaaaatggt
     7501 ccagcccttc acgccatcct tccggtcctt cacgctgcgt tcgccagacg cgggccggat
     7561 cctcatcatg cagatgcacc ccttcacggt ttagcccttt cacaatcacc gcgtaatcca
     7621 ttacctccag taagggcgca tcgtttggcc catcgcccag gccaagtgtg gttgggcgtt
     7681 tgcctgacaa ttgttgatag gtcgcgataa tccagttggc agcctgatct tttccggcag
     7741 aggcatccag gacgtgccag aagcgcgcac cttgcataaa ctgtaagccc agttcgttca
     7801 gacgagcggt aaattgtgcc atacgctcgt cactgtcgcg ccagattagc gttaccgacg
     7861 cctcatgaag ctgcgtcagc gccgcctggc tacggcttaa tcccgtccat tcggcgatgg
     7921 ttgcatcgtc gacatcatca aaagtcgtga atttaaaatg ttctttctcg cgtagcgtat
     7981 ttaaaacctg gctgatttcg ccatggctaa tacctgagat gatgcgtgga aaaccgtcta
     8041 tctcctgcca ttgttcagca agctggatca ctgcgccatt ctctgcaatc agcggtaaac
     8101 cttgtagccc caacattttt tgcaagtaca gcatttccgc tgatgtttta ctgctacaga
     8161 gaatgacggg aacatttgct tcgcgtaaac ggctgagcca gggggctgcc ggttgccagt
     8221 cataactatg actgtccagc agggtgccat caagatcgct aaaaaccagt agtggttgtt
     8281 gaattgaaaa catagtcgcg cagtactccc cttaccagga tgattctcaa aacagcacaa
     8341 attatcaaag atgatttttt cgggagcgat cccgctgacg ccagagccac caaggggtga
     8401 cgtgcgtcac atttctattc ataagtagcg ttaatcattc atatagcgaa tattttcttg
     8461 tcagcgaaaa aaattgcgga taaggtgatg aacacatcag atttcctggt gtaacgaatt
     8521 ttttaagtgc ttcttgctta agcaagtttc atcccgaccc cctcagggtc gggatttttt
     8581 tattgtgcat tcaacgattc acttcagaga cgctgaagtc atgaatatcc agttcgaagg
     8641 catccgccaa ttcacgccat gtatgatatt cacgtccatc gacgctaacg tgttcagaat
     8701 cgttctggct gcgatgaact tcgctttcgc taatttcatt gatcgccgcc agcagggcat
     8761 cgacatcgac gctgacctca cgttttgcgg tatcgctgta ctcttttgcg gttttcatca
     8821 tctttcctcg caaccgttac tctgttgttg ccgccagtcg ttaagtccgc ctggcatctc
     8881 ttaagtatag accgtcgaga aaatcagcac tcgcgcgcgc ctggcgctgc cagaaaaacg
     8941 aaattgttct acactggcac aaagccacag gaggaaaacg atgaaggtga atgatcgggt
     9001 aacagtcaaa acggatggcg gtccgcgtcg tcctggcgtg gtactggcag ttgaggagtt
     9061 tagtgaaggc acaatgtacc tggtttcgct ggaagactac ccgctcggca tctggttctt
     9121 taatgaagca gggcatcagg acggtatctt tgtggagaaa gcagagtaat ctaattcagc
     9181 ctgactggtg ggaaaccacc agtcagaatg tgttagcgca tgttgacaaa aataccatta
     9241 gtcacattat ccgtcagtcg gacgacatgg tagataacct gtttattatg cgttttgatc
     9301 ttacgtttaa tattaccttt atgcgatgaa acggtcttgg ctttgatatt catttggtcg
     9361 gagatttgaa tggttccctg acctgccatc cacattcgca acatactcga ttcggttcgg
     9421 ctcaatgata acgtcggcat atttaaaaac gaggttatcg ttgtctcttt tttcagaata
     9481 tcgccaagga tatcgtcgag agattccggt ttaatcgatt tagaactgat caataaattt
     9541 tttctgacca atagatattc atcaaaatga acattggcaa ttgccataaa aacgataaat
     9601 aacgtattgg gatgttgatt aatgatgagc ttgatacgct gactgttaga agcatcgtgg
     9661 atgaaacagt cctcattaat aaacaccact gaagggcgct gtgaatcaca agctatggca
     9721 aggtcatcaa cggtttcaat gtcgttgatt tctctttttt taacccctct actcaacaga
     9781 tacccggtta aacctagtcg ggtgtaacta cataaatcca taataatcgt tgacatggca
     9841 taccctcact caatgcgtaa cgataattcc ccttacctga atatttcatc atgactaaac
     9901 ggaacaacat gggtcaccta atgcgccact ctcgcgattt ttcaggcgga cttactatcc
     9961 cgtaaagtgt tgtataattt gcctggaatt gtcttaaagt aaagtaaatg ttgcgatatg
    10021 tgagtgagct taaaacaaat atttcgctgc aggagtatcc tggaagatgt tcgtaggtat
    10081 tatttttcgg ataatcctta ggataacatg ataaacgtta cggaattata ttaatggcaa
    10141 ttcactaata atatcagcca gcaaattaaa aatttcactg aataaatgtt cgcaaaaagg
    10201 cgcaattaac ggcattaatg ccgccattaa agagatgccg acagtcagag ttaatggaaa
    10261 tccaataaca aaaatggata attgcggggc catacgatta agtaaaccta atgccagatt
    10321 cagtgtcagc agcagagtaa tgagcggtaa cgccagcatc agcccgttaa ggaaaatcaa
    10381 actccctgct ttggtgagtg ccagaaacgc attgctgttc aacggttcgc caccaatcgg
    10441 cagggtgtga aaggtatcga ccagcagtga aatcaaccat aaatgaccgt taaatgtcag
    10501 gaacagcagt aacgccagca tatccatgat acgcgctaaa acgggcatat taagatggct
    10561 ggccggatcg acaaacgtcg caaatgacag ccccatttgc agaccgataa tttcgccagc
    10621 ggttcgcaca gcggcaaagg caaattgcat ggtaaaacca agcgcaatgc cgatcaggat
    10681 ctgctgcacg gccagccaca gagcaaagaa cgaaaaaaca ggaacatcgt tggcaggtaa
    10741 tgatggggca atggcgaacg tgatcatcat tgccagaccc agttttaccc gtttcggtac
    10801 gctgcgttcg ctcagaatcg gcgcggtgga gatcagcgcc agcacgcgca gtaacggcca
    10861 gaagtacagg cttaaccagg atagccattg ttcgcttgtc acctgcaaca tagtacggct
    10921 acccgatgat atacggcagg ttagtgaaca aggtgcggac gtaatccagc aacagattga
    10981 gcatccacgg tccggcaata ataatggcga taaatacggc gatgattttc ggaataaacg
    11041 acagcgtcat ttcgttaatc tgcgtggcgg cctgcaaaat actgatgata aggcccgtga
    11101 ccaacgctac caacaatagc ggggcagcca gtgccagcgc gactttcatc gcttcagtcc
    11161 ccatcatcat gaccgattca ggtgtcattt tgcctctcta gctgtaaaag ctctgcgcca
    11221 gcgaaccgac cagcaattgc cagccatcca ccagtacaaa cagcatcagt ttaaagggca
    11281 gagcaatggt ggcgggggga accatcatca tccccaatgc catcaacacg ctggctatca
    11341 ccaggtcgat aatcaaaaaa gggatgaaaa tcgtgaagcc tatctgaaat gcggttttca
    11401 actcgctggt cacgtaggcc gggagcaaaa tgcgcatcgg cacggcttca ggtccctgca
    11461 acgggccggt attcgccagt ctggcaaaca accctaaatc tgcctcacgg gtctgacgca
    11521 gcataaactc acgcagcggc tgcgcccctt tttccagcgc ctcctgcatt gatattttct
    11581 cttcgctgaa tggctggtac gcatcgacat aaattttgtc gatcaccggt gacataataa
    11641 aaaaggtcaa aaacagtgcc agccccagca atacctggtt aggtggcgcg gagggtgttc
    11701 ccagcgcgtt acgcaataaa ccaaaaacaa tgatgatgcg ggtgaaactg gtcatcatca
    11761 gtaaaattgc cggaataaac gtcaacgagg tgatgaacac cagcgtctgc accgggagcg
    11821 accagctttg tccaccgcca ggcagcggct ggctggtgat acccggcagt tgcgcgaagg
    11881 cgaggggcgt aatcagccag agaaggacag gtgcgacaga caataaacga cgcatcagga
    11941 tctcccgcta cgcttaagca aatttttcat gaccgactga aaatcggtct gcggtatctc
    12001 ttctgttggt gctgaagggg gaagtttatg cagcagattg atttgccctg cggtaacgcc
    12061 gagcaccagc cgtgcatctt ccacatcgac caccacaacc cgttcacgcg cgcccagcga
    12121 agcactggcg ctaattttca gaccgttaac gccagtgcgt ttcggggcaa atcccaaccg
    12181 ttttaccagc caggcagcag cgaggatcag ggcaataatg gcgatgagtg cgccgctcac
    12241 ctgcagcagt ggcgcagcag aaaccggcgc ggaagattgc acagtagcgt ggttattcat
    12301 cactaacggc tcaggcggcg cattcgctca gacggagtaa tgatatcggt gatccgcacg
    12361 ccatatttat cggcaacgac caccacttcg ccctgggcga ttaaataacc gttgatcaga
    12421 atatccagtg gttcgcccgc cagaccgtcc agcgccacga cggacccttg cgtcagacgc
    12481 aacagctctt tgatggtcat tcgtgtgcgg cccagctcga cggtcagctt gaccggaata
    12541 tccataatca ggtcgatatc ctgcaacgtt ccgctgacat caccaccgcc aaattgctgg
    12601 aacaccgcgt cggcagcgct tttgctgctg gttgattttt gttcgctcaa cgcttcagcc
    12661 cacagatcgt ccattgcgcc gttgttgtca tcggccggat tattcatgtc actcatttgg
    12721 gctgttcctc gttcagagaa tttaaaatcg ggttaatcaa atgttctatc cgtaacgcat
    12781 actgaccgtt gagagtgccg tactgactgg tcagcaccgg gacgccgtca acatgggcga
    12841 tgatgcggtc gggtttttct atcggcagga cgtcaccggg tttcagtttt aaaatctgcg
    12901 acaggcgtag cgagatatcg gcaaagttgg cgaccagctc cagctgtgaa tgctgcacct
    12961 ggcgcaccag gttatcgcgc cagttctgat cttcattacg cgagttttcc agcggcgggt
    13021 taaccaacaa ttcccgtagc ggctcgatca tgctgaatgg caggcagata ttaaattcgc
    13081 cggtcaggtt gccaatctcc acatggaacg gcgtgttaac cacaatgtcg ttcggcgagg
    13141 tggtgatatt ggtaaatttc acctgcattt ccgaacgcac gtactcaact tccagcggat
    13201 taatcgcctt ccatgcgtcg ctatagcctt caagcgccag tttcaacatg cggttgatga
    13261 cgcgctgttc ggtatgggta aactcgcgac cttccacttt ggtcgggaag cgtccatcgc
    13321 cgccaaacag gttatccacg gcgataaaca ccagactcgg tgagaacacc accagcccag
    13381 tgccgcgcag cggttttaga tggataaggt tcaggttggt cggcaccggc aggttgcggg
    13441 caaattcatg gtacggctga atgcggatgg ccccgacggt tatatccggg ctacgacgca
    13501 gcaggttgaa cagccccata cgaaaatggc gggcaaagcg ctcattaatg atttccagcg
    13561 cctgcaaacg ttcgcgcaca acccgtcgtt gggtattcgg atcgtacgga cgaatgtcac
    13621 tttcgccgct aacactggct gtcggttcgt ctttgacttc gctgtcacca ttcaacagcg
    13681 catcaatttc agcttgagaa agaatactat cgcccatgtc gttatcgcag aataaaagcg
    13741 gtatacagca cgtcggtgac atcctgtttc ggttgcccgg caacaagcgg ggtggaaagt
    13801 gtggttttaa tctcggcaat caggtttttc ttgccttctt ctgtcgccag tacggcagca
    13861 tcctgacgcg aaaacagtaa cagcaaacgg ctacggactt ccggcaaata ctcactcagc
    13921 cgcgagcggg tagcttcatc tttcaggcgc agggttatgc cgatataaag cacgcgatcc
    13981 gcatcgccca gattgaccgt aaaggtatcc agcgcataga agaccgggga gggcacgacg
    14041 cgttgctgcg ctttgtcgtc agcagcaacc tgatgcgaat gccagtagct gtaacctgcg
    14101 ctggcacagg ccgcgagggt aatgaatacc agaatcggga tccaaagcga tcgcttgctt
    14161 ttcttgctta tcgcgtaatc agtcatgtgt tgcggtcttc ctgtgtcgct actgcttatc
    14221 ggctaattat cctgcgtctt gagcgcaaca aagcggggaa aagacgcgga ttacggtgct
    14281 acctctgacg ttaggcgaaa atatcaacgc cgctgttgcc tgttacgcgc ccttgtaaag
    14341 agacgggaac cggaagcgta tcgtcgtctt cccccgccag aggttcatgg tttactgtgc
    14401 gttggctttg ctgttgttgg gaagcggcct gctgctgacc actaaagctt tcgccactga
    14461 tgttgctttg ccctaactga atgccacttt cggccagctg cgtacgcagt accggcagcg
    14521 ctgcttccag ggcggcgcgt acatgctgat gcgatgaaac catctggatt tgcgcctggt
    14581 tatcatccac tttgagggag atttgcactt cacctaaatc ctgcgggtgc agacgcaact
    14641 ctgcactttg ttgcccctgg cgggtgaaca gcgaaatatg ctggcttaat gattgttgcc
    14701 attcgtgaga acccagcggt gcactcaaaa caggcgcggc gacggtgggc agtggctgtg
    14761 tctggtgtgg agtgattagc gggctggcgg cagcagtcac cggcgaaggc gtgctgatga
    14821 cttccgcttt actctgggct tctgctacct gcggtgttaa tggctgagct ggtgtgccgg
    14881 gggcatcatc aggctgtgct gttgtgagtt gcgcagaagt cagttttgtg aagagcgttg
    14941 gtttctctgc cggtaacacg gttgacggca catcagtcac tttgggcgta ttgtcaaaac
    15001 ccggcaacat cgcaaaaagg gcgctcaggc ttgcggtgac gtcttcattc agatcatccg
    15061 ctttttcgtc ttttgtcgta tttttgtcag ccaccgcagc caacgccatc gtctgagcgg
    15121 tggttaacgg tgttgatgtg gattgttcgt cgttgatgac aggcggtgtt tcatccacag
    15181 ggatcagtaa attagcttgt tgcgcgtcgg aaacaatatc gctgatcagc ggctcgcctt
    15241 ttgtcgtggg cttatctgtt gccaccagca actggggggc cgctttgtcg gtagttgtct
    15301 cgcctgctaa tgcttcgctc aacaacgcga gaaaatcttg tgcagcatcg ctggctttgc
    15361 cgccaggcaa tgtggtggtg tcaacgtcgg cggtaatcaa gggcgctaag cgaatcattc
    15421 aggtttcctc atggcggcgc gctgggcgaa ctcatccatc tttttctgat cgaggcggtt
    15481 ttctgcaagc agtgccgccg tggattgccg ttcctgcagt gtctgccagg cctgcaaacg
    15541 ttgtttcttt tctcgccagc tgttcagggc aatgtcaact ttctgcgtcc actgattaag
    15601 ttgctggcga tgctgagtaa tggctttttc cagcgtctgg ataaactgct gatagttgat
    15661 ccagcggttg ctggtcatcc cggcactcat atcgctgttg aggttattgc gatattcatt
    15721 ctgataatcg atcagcattt tgagctgttc ttccgcctgc tgacacccgc gacgcatttc
    15781 acccagcagg cgcgcggcat cctctacctc tttttctgcc agatctttca gggtcgccag
    15841 cgcgccatgt tctgccatct gccgttatct cctgggttat gacactgtcg ggaaaatacg
    15901 ctccagcccc tggagagacg cttcccagtc cgcgcgttca aaaatgcctt gttgcaaata
    15961 gccctccagc tgcggccaca gggcgatggc tttgtcgagc atcggatcgc tgcctttggc
    16021 atacgcgccg acgctaacca gatcgcggtt acgctgaaaa ctcgacaaca gctgtttgaa
    16081 ggtgcgcact cgcgcgtaat gttgctcact gatcaacgcc gtcattgcgc ggctgatcga
    16141 cgcttcaata tcgatagccg gatagtgccc ggcttccgcc agtcggcgag acagcacaat
    16201 gtgaccgtcg aggatcgccc gcgcggagtc tgcaatgggg tcctgctggt catcgccttc
    16261 agtaagcacg gtataaaacg cggtaatcga gccgccgccg ctaatgccat ttccggcacg
    16321 ctcgaccagt gccggtaatt tggcaaacac cgacggtgga taacctttgg tggcgggtgg
    16381 ttcgccaatc gccagcgcaa tttcacgctg ggccatcgcg tagcgggtga gggagtccat
    16441 aatcagcaac acatgctgac cacgatcgcg aaaatcttcg gcaatgcgcg tggcataggc
    16501 ggcaccttgc attcgcagga gcggagacac atccgccgga gcggcaatca ccactgatcg
    16561 tgcacgccct tcggcaccga ggatattctc aataaaatct tttacttcgc gcccacgttc
    16621 accaatcaaa cccacgacaa tgacatcggc gcgggtgtaa cgtgccatca tccccagcag
    16681 cacactttta ccaacgccgg acccggcaaa cagccccata cgctgcccac gcccaacggt
    16741 aagcagggca ttgattgggc gcacgccggt gtccagcaca tgttcaatcg gtgtacgttg
    16801 caacgggtta aatggcgggg taatcagcgc accggtttcc gtcgtatcgg gggagggcag
    16861 gccatcgagc ggtttaccgc tgccgtccag aacgcgacct aataacgccg gaccgagcgg
    16921 caactgcttg ccgctttgca gtccttcagc cgaaatgttt ttggcataaa cccgcgcgcc
    16981 gggcaggaca ccttccactt cctccagcgg cattaaaaac agccgttgac cgttaaagcc
    17041 aacgacttcg ctttctactt cgtgcgtttc gctgccgttc tggcgttcaa tgacacaggt
    17101 tgcgccgagc ggcaattgta atccggtggc ttccagcacc agcccggtag cgcgggttaa
    17161 tcgcccgtag cgacgtaccg caggcaactg cgccattttg gcttcaaagt tatccagcgt
    17221 ggttagccag cgagtcaggc gcgtggtcat tacaccactc ctggtgctgc cagacggcag
    17281 agttcttgcc agcgagtcag gcgcgtggtc attacaccac tcctggtgct gccagacggc
    17341 agagttcttg ccagcgagtg gcgacactgg cgtcgagatc gccttcatcg gcggagactt
    17401 tacagccgcc aggatggagg gtgggatcgc cccgcaagcg ccagccatgc aaacttaagg
    17461 tagcgccgag catatcatcc acacgttgca gatcatccgg gtgcacgcgc agctgtggtt
    17521 taccgctgaa taacggttct tgctgcaaca actgttggat ctgtttgatc agtgccgagt
    17581 tatccaccgt tggcgtctga ccgatgacct gacgtgccgc ctccagcgcc atctgcatca
    17641 ggcgcgacgc tatcacacta tcaagtgcat caagggtagt ttgaaattcg ctgaccagtt
    17701 gctgcatccg ggcatgaatt ggcgcttgtt gagacttcgc ctctgccaga ccttgctcca
    17761 gcccctgggc cagtccttcc tgatagccct gcttatgacc ttgctggcga ccttcggcaa
    17821 tacccgcctg ataaccttgc tcatgggcct gcatttgcag ttgcgccagt tgctgctcaa
    17881 ggctgggttc agcctcttca atgatggttt cttccgactc gaccatgggc acaaactctg
    17941 cctgtggtgg cgcgagatcg tccggcgtcc aggttttcca gggcagatta tcagacatag
    18001 gtatcctcgc cgctgccaat taccatctcg ccagtttcgg caaggcggcg cacaatcagc
    18061 agaatcgctt tctgttcgtt ttccacctgc gacagacgca ccggaccacg gttggcgaga
    18121 tcgtcgcgca gaatatcggc ggcacgctgc gacatattgc gcaggaattt ctcgcgcagt
    18181 ggctgctcgg cccctttcag cgcgatcaac agcgattcgg aatccacttc ctgcaacaga
    18241 cgctgaatgc tgcgatcgtc gacatccacc agattttcga acaggaacat ctcgtcgatg
    18301 attttctgcg ccagctcgcc gtcgaattca cgcacggcgg taataacggc ttcttcctgc
    18361 tgagttttca tcaggttgat aatttcggct gccgttctca cgccgcccat tttgctgcgc
    18421 ttgagattct gaccgtcgag caagccattc agtacttcgg tcagttccgc cagcgcggct
    18481 ggctgcacgc cgccaaaggt ggcgatacgc aacatcacgt cgtggcgcag acgttcatcg
    18541 aacaacgcca gaatatcggc ggcctgggcg cgcttcagat gcaccagaat ggtggcgata
    18601 atttgcggat gctcatcacg aatcagatcg gcggcgctct gcggctccat aaagttgagc
    18661 gtttcaatac cgctggcggt atcgcgagtt tcgagaatat cttccagcag gctggcggca
    18721 cgttcctcac ccagagcttt gaccaatacc gaacgcagat aatcgttggc gttgatattc
    18781 agtgcggcaa actgttcagc ttcttgctca aactccgcca gcacatcggt tagctgcttg
    18841 ttggagatct gcgtgacgtt cgccattgca gcgctcaggg tttgcacctc gcgctgggag
    18901 aggtgcttga acacttctgc cgcccggtct tcgccaatgg tcatcagcag gatgacgctt
    18961 ttatcggtgc ctgtcaggtt actcatgatc gttatttatc cactggcgaa tgaccagcgc
    19021 taccacgcgc ggatcgttat cagacatttc acggatacgc tggctcatga cttctgcccc
    19081 cagacgttgg ttagcgcgcc gttgttggag ttgttcgtct ttgctcaggc ggacttccac
    19141 cgcatcttcc acttcctcgc gggcctgcgc ctgttgctgt acagctttca tcgcctcagc
    19201 gcgacgtgtt agctgcgggc gtaccgcttt acgccacagc agccacgcca ccagcagtac
    19261 cagcaaccag cgaccggcag ccagtaactg atcgataaac gcttgctgtt gccagaacgg
    19321 cagttctccg ccgctttcgt cactactatt gaacggcgag ttaacgacat tgagcgagtc
    19381 accgcgtttt tcagaaaagc ccatcgcctc gcgggtcaga tcttcaattt gtttcatctg
    19441 ttcgttgctg agaggcaatg gtttaccgtc tggcaaggtt ttgtaattca ccactaccgc
    19501 gactgacaga cgttgcacat cgcccacgtt cattttggta tgacgaatag tgcgatcgac
    19561 ttcgtagtta ctggtttcat tccgctgtgt gctacgcggc ccactattgc tggtggtgct
    19621 cgcctgctgc tggcggttat tttgatttgc cggaggcgtg ctgattggcg cgttattcgc
    19681 aggagccggt tgattcgaca acgcccccgg tacgccgccc ggataaccgg aaccgctttg
    19741 ctcgctctca ttaagctggc gtgaacgaag cgccgcctga gattcatcac cattagggcg
    19801 atactgttct tccgtttgtt ctttactggc gaagtccagt tgcgccgtga cctgggcgtg
    19861 aatattaccg ttaccaacaa taggcgacag gattgcttca atacgccgct gaatacggcc
    19921 ttcgacatcg ctggcatatt tcaattgagc gtcattaaga tcgcgccagc tggtattgga
    19981 ctgggttaac agatgtccgc cctgatccac cagcgtgacg tttcccggcg gcagaccagc
    20041 aacggcgctg gaaaccagat gcacaatggc gctaatttgc ccttcatcga gtgcgcggcc
    20101 gggtaacaga tttaccgtca ccgatgcaga aggggatttt tgttcacgga cgaataaaga
    20161 cggtttcggc attgccagat gtacgcgcgc cccttttacc gggccgatag tttcgatggt
    20221 acgagaaagc tcgccttcca gcgcccgctg ataattcacc tgttcgctga actggctgat
    20281 accaaacttt tcctgatcga gtagttcgaa accgaccgcg ccgcctttgg gcaaaccttg
    20341 ttgcgccagg cgcaggcgca gctcgtgaac tttatctgcc ggaacttcaa tagcgccgct
    20401 ggcttcgctg aagcggtaag gaatattcat ttgcgtcagt tggctgacaa ttgcgccacc
    20461 atcctgatcg gaaagattgc tgaataatgt gcggtagtcg ggggctttcg cccacaggat
    20521 cagtgcgacc atgaccgcca ctgccgcgga accggcaaca atcaatggaa ttttcggatt
    20581 cgcacgcagg cgattaagcc accaagagat tttgtctggg ctgcagtcgc attcatcgcg
    20641 cacctcgtgg ctgattatgg acggcgttat tggcaaagtg gaacaagact cactcccggg
    20701 tcagcaaact cgaaaaattg acgctcccat tatttgtgct ttcgtttttt tctatccgcc
    20761 aataaacccg tttttttgtt gctatttagc gcctttgtct tattgactta ctggtaggct
    20821 ttgctaccag aaattatcca ggagatgaaa atgtcagcga tacaggggat tgaaggggtt
    20881 atcagccagt tacaggctac ggcgatgagt gcgcgtgcgc aggaatcact gccgcaaccg
    20941 accattagtt ttgccgggca gctgcacgcc gcgctcgatc gcataagcga tacacaaaca
    21001 gccgcccgca cgcaggcaga aaaattcact ctcggtgaac ccggcgtggc gttaaacgat
    21061 gtgatgaccg atatgcaaaa agcctcagtt tctatgcaaa tggggattca ggtgcgtaat
    21121 aagctggtgg cggcatatca ggaagtaatg agcatgcagg tataggttgt gtaacctgtt
    21181 gttaatgaca tccgatctca ccgcgtgggg catggatggg gcaaacttac tcattatctg
    21241 gtttgaaatg gatacctgat cctgaaagtt ttctgccatc cacgatccgt atacacgata
    21301 aaccccctga acatcagagt gtctcatttg acacatcacc acgtcggatg ttgttacgac
    21361 cacagacgca acagtaacct ctggataatt ttatctttgt tgatgttttt attcatgttc
    21421 cgggctatgg atatgagcga tttttgatag tggtaatttt attgtgtttt agaatgcgcc
    21481 ctgggcaata taatttatta aacaggagtg taaatgtttc cattaaatga tctgtcgtta
    21541 aaaactcagt cggttcaatt aaataaaatc acatcgaata ctgagtctac gataaaacaa
    21601 cacgagttag tatctgatga tgcaatcata aatgaattat caagtgagtt agtcagttgt
    21661 cttggaaatg ataagtttac accagttagt gaagactgca acttactgaa tatgctgtct
    21721 gaatttaagt tattgagaga gcaatgtttc aggtggggta attatactct attgtttgaa
    21781 aattatgagg cttatgataa gacggggtct atcacgatag aaaaaaatca gggggagggg
    21841 actttaccca ttcggcataa attagagttt atatcgacta atattgcaga gttgctggac
    21901 aagttaacga aaattacaga tgccagactt tgcaaaggct tcagtgactg ggctagttca
    21961 gtcaaagaag gcggatcgaa tgacctcaaa gaaaatgtag atagagcgtt ggtgagaatg
    22021 tttaaatgtg ttaagcttca cagtaatgaa cttgacttat catacctatt tttgggttct
    22081 gtgccgcctc ttccagattg gattgaaatg cttagccttg tttataatga acttgattca
    22141 atacaggttc ccgaatcatg caaagaatta gaactcgatt tcaataacct tacagaattt
    22201 ccacaagtac ctgatggaat taccctgatc tccgtaaata ataacctgat atcgcatatt
    22261 gactcatttc cgccaaaggc taagaaaatt tttattagtc acaataagct atcggaaaca
    22321 ccagcaatac cagacaccgc taaggttttt gattgtggct ataataagat acaagaaatt
    22381 cgatacttcc ccaaaaatct gaaagaagcg cgtattggat ataataatat tgaggttgtt
    22441 cctgcgatac ctggcaattt aaaaatactt tttatggaat gtaatcctat taaagaggca
    22501 tttttaatgc catggactct gacagggatc tgctatgaaa tatcgcagcg aaaatatatt
    22561 gttacgaatc cagccgatta tgataaatat tccgatatgg ttaaaaagca tgtaatagat
    22621 ggtgaggaac tcataattaa atattttatg taattaatta ggcgtaattg tgtcactgga
    22681 atagaggttt cttgtgacgc atgtggaagg agcgtctatg gacaaggcgt ttcctgctgg
    22741 tgataaataa aggcgcagca cgtcgaatgc aagaatgcgc tgcgtctggc tcatggcaat
    22801 tcattatctt cgcatagcgt caatgacaaa aatcgactgt gtggatcaaa ttgataatca
    22861 gcaaacggtt cgcaatcaac gaaaccatgc ttcaaataaa gttgatggca ggccgtaaat
    22921 ccagcctgtg tacccgtttc taagctcagg cgatgaaggc atctgtcatg ggcgacctgc
    22981 aaaatgtggc gtaaaatcag actggcgaca ccccgacgta aataatttgg cgcggtacgc
    23041 attgatttca gttcgccatg tttatcatcc agcaacttta gcgcacccat tcctgcgagt
    23101 tgttcgcctt cccatactga cctgttgacc tggcttgttt aatgacatcc gatctcaccg
    23161 cgtggggtta tggatggggc aaactcactc aatttctggt ttaaaatgga aacctgattc
    23221 tgaaagtttt ctgccaccca cgatccgtat acgcgataaa ccctctgatc atcagaatgt
    23281 ctcatttgac acatcaccac gtcggatgtt gttacgacca cagacgcaac agtaacctct
    23341 ggataactta atcttcgttc ttatttttat t
//
LOCUS       DAZKIP010000068        23361 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZKIP010000068 DAZKIP010000000
VERSION     DAZKIP010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.068"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..1225
                     /locus_tag="ACHOUK_002891"
     CDS             <1..1225
                     /locus_tag="ACHOUK_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFP9898296.1"
                     /translation="IPQMNYLMVVVALFFLNAVIFLFMLMKYFTNKQILPTLILSLAF
                     LSGLIYLVETIVIIHKPINGSTLIQTKSNDVSIFYIFRQLSFICLTSLALFCYGKDNI
                     LDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHTATWGINYTKI
                     LVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYISRGIE
                     VSSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDFCV
                     MLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGEVFGLLFTELNS
                     AQAKIIAERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRNISLVMTEADKALR
                     EAKSEGGNKVIIHHI"
     gene            1406..2896
                     /gene="dgcJ"
                     /locus_tag="ACHOUK_002892"
     CDS             1406..2896
                     /gene="dgcJ"
                     /locus_tag="ACHOUK_002892"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFP9898297.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSILQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKLINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            2939..3442
                     /gene="yeaK"
                     /locus_tag="ACHOUK_002893"
     CDS             2939..3442
                     /gene="yeaK"
                     /locus_tag="ACHOUK_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFP9898298.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGADFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(3443..3547)
                     /gene="yoaI"
                     /locus_tag="ACHOUK_002894"
     CDS             complement(3443..3547)
                     /gene="yoaI"
                     /locus_tag="ACHOUK_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFP9898299.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            3717..4163
                     /gene="yeaL"
                     /locus_tag="ACHOUK_002895"
     CDS             3717..4163
                     /gene="yeaL"
                     /locus_tag="ACHOUK_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFP9898300.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIATGLVSLIVGKQ"
     gene            complement(4120..4941)
                     /gene="nimR"
                     /locus_tag="ACHOUK_002896"
     CDS             complement(4120..4941)
                     /gene="nimR"
                     /locus_tag="ACHOUK_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFP9898301.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     YTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RAMVEMMAKEPVEWGALGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPWQ"
     gene            5038..6219
                     /gene="nimT"
                     /locus_tag="ACHOUK_002897"
     CDS             5038..6219
                     /gene="nimT"
                     /locus_tag="ACHOUK_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFP9898302.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6274..6621
                     /gene="yeaO"
                     /locus_tag="ACHOUK_002898"
     CDS             6274..6621
                     /gene="yeaO"
                     /locus_tag="ACHOUK_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFP9898303.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6643..6897)
                     /gene="yoaF"
                     /locus_tag="ACHOUK_002899"
     CDS             complement(6643..6897)
                     /gene="yoaF"
                     /locus_tag="ACHOUK_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFP9898304.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRLDHPAGEK"
     gene            7080..8105
                     /gene="dgcP"
                     /locus_tag="ACHOUK_002900"
     CDS             7080..8105
                     /gene="dgcP"
                     /locus_tag="ACHOUK_002900"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFP9898305.1"
                     /translation="MSDQIIARISQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNQKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHS"
     gene            8138..8236
                     /gene="yoaK"
                     /locus_tag="ACHOUK_002901"
     CDS             8138..8236
                     /gene="yoaK"
                     /locus_tag="ACHOUK_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFP9898306.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8239..8313
                     /gene="yoaJ"
                     /locus_tag="ACHOUK_002902"
     CDS             8239..8313
                     /gene="yoaJ"
                     /locus_tag="ACHOUK_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFP9898307.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8372..8620)
                     /gene="yeaQ"
                     /locus_tag="ACHOUK_002903"
     CDS             complement(8372..8620)
                     /gene="yeaQ"
                     /locus_tag="ACHOUK_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP9898308.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8767..8949)
                     /gene="yoaG"
                     /locus_tag="ACHOUK_002904"
     CDS             complement(8767..8949)
                     /gene="yoaG"
                     /locus_tag="ACHOUK_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFP9898309.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(8953..9312)
                     /gene="yeaR"
                     /locus_tag="ACHOUK_002905"
     CDS             complement(8953..9312)
                     /gene="yeaR"
                     /locus_tag="ACHOUK_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFP9898310.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(9485..10123)
                     /gene="leuE"
                     /locus_tag="ACHOUK_002906"
     CDS             complement(9485..10123)
                     /gene="leuE"
                     /locus_tag="ACHOUK_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFP9898311.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGMATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(10250..11173)
                     /gene="dmlR"
                     /locus_tag="ACHOUK_002907"
     CDS             complement(10250..11173)
                     /gene="dmlR"
                     /locus_tag="ACHOUK_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFP9898312.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            11276..12361
                     /gene="dmlA"
                     /locus_tag="ACHOUK_002908"
     CDS             11276..12361
                     /gene="dmlA"
                     /locus_tag="ACHOUK_002908"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFP9898313.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            12612..14222
                     /gene="yeaV"
                     /locus_tag="ACHOUK_002909"
     CDS             12612..14222
                     /gene="yeaV"
                     /locus_tag="ACHOUK_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFP9898314.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            14254..15378
                     /gene="yeaW"
                     /locus_tag="ACHOUK_002910"
     CDS             14254..15378
                     /gene="yeaW"
                     /locus_tag="ACHOUK_002910"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFP9898315.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            15434..16399
                     /gene="yeaX"
                     /locus_tag="ACHOUK_002911"
     CDS             15434..16399
                     /gene="yeaX"
                     /locus_tag="ACHOUK_002911"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFP9898316.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTPVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(16453..17568)
                     /gene="rnd"
                     /locus_tag="ACHOUK_002912"
     CDS             complement(16453..17568)
                     /gene="rnd"
                     /locus_tag="ACHOUK_002912"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFP9898317.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(17650..19335)
                     /gene="fadD"
                     /locus_tag="ACHOUK_002913"
     CDS             complement(17650..19335)
                     /gene="fadD"
                     /locus_tag="ACHOUK_002913"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFP9898318.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTLVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVSPGQPGELCVRGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(19540..20121)
                     /gene="yeaY"
                     /locus_tag="ACHOUK_002914"
     CDS             complement(19540..20121)
                     /gene="yeaY"
                     /locus_tag="ACHOUK_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFP9898319.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQATGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGHGGWGWYNPGPARVQTVVTE"
     gene            complement(20161..20856)
                     /gene="tsaB"
                     /locus_tag="ACHOUK_002915"
     CDS             complement(20161..20856)
                     /gene="tsaB"
                     /locus_tag="ACHOUK_002915"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFP9898320.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(20914..22824)
                     /gene="yoaA"
                     /locus_tag="ACHOUK_002916"
     CDS             complement(20914..22824)
                     /gene="yoaA"
                     /locus_tag="ACHOUK_002916"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFP9898321.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            22956..23300
                     /gene="yoaB"
                     /locus_tag="ACHOUK_002917"
     CDS             22956..23300
                     /gene="yoaB"
                     /locus_tag="ACHOUK_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFP9898322.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         5717 a         5956 c         5675 g         6013 t
ORIGIN      
        1 catcccacag atgaattacc tgatggttgt tgtggctttg tttttcctta acgccgtcat
       61 ttttcttttc atgttaatga aatatttcac taacaaacaa attttaccaa cactcatttt
      121 aagccttgca tttttaagtg gccttatcta tttagttgaa accattgtaa ttatccataa
      181 accaattaac ggcagtacac tgatccagac aaagtcgaat gatgtttcta ttttctatat
      241 tttccgccaa ctcagtttta tttgtttaac ctcgctggcg ctcttttgtt atggaaaaga
      301 caacatcctt gacaacaata agaaaaaaac tggcattctg ttgctggcgc tgatcccttt
      361 tttagttttt ccccttctgg cacacaatct gagcagttat aacgctgact attctttgta
      421 tgtcgtcgat tactgtccgg acaatcatac tgcgacctgg ggaatcaact atacaaaaat
      481 attggtttgt ctgtgggcat ttttactgtt ctttattatc atgcgtacac gattagccag
      541 cgaactatgg ccgttaatag cattattatg tctggcatcg ctatgctgca acttacttct
      601 actgactctg gatgagtata attataccat ctggtatatc agtcgcggga ttgaagtctc
      661 cagtaaactg tttgttgtgt cttttctgat ttataacatt tttcaggagc tgcaactctc
      721 cagcaaactg gcagttcatg atgtgctgac caatatttat aatcggcgct actttttcaa
      781 cagcgtagag tcattattgt cgcgacctgt tgttaaggac ttctgtgtca tgctggttga
      841 tattaatcag ttcaaacgca tcaatgccca atggggacat cgtgtgggtg ataaagtgct
      901 ggtttcaatt gtcgatatta tccagcaaag catccgcccc gatgatattt tagcgcgact
      961 ggagggtgag gtgtttggct tgctatttac cgaactcaat agtgcccagg caaaaatcat
     1021 tgcggaacgt atgcgtaaaa atgtcgaact cctgaccggc tttagtaaca gatatgatgt
     1081 tcctgaacaa atgaccatca gtattggcac agttttttca acgggtgaca cgcgtaatat
     1141 ctcgcttgtc atgacggaag cagataaagc cttacgcgaa gcgaaaagcg aggggggcaa
     1201 caaagtgatt attcatcata tttaagtgca aaaatattca gagccatgct ttttgcgtgg
     1261 cttttgcata caatttatta ctattacccc taaatttccc ctccccagcg gcgcagagat
     1321 gagtataatt agcgcccctg tgccaggccg caatcgaact ttatctggtt ttctcgtttc
     1381 actaaccgaa ggagtgccat ttatcatgaa attgcaccat agaatgctcc ggcattttat
     1441 cgccgcaagt gtcattgtgc tgacatcttc cttccttatt tttgaacttg tcgccagcga
     1501 cagagcaatg agtgcctatc tgcgctatat cgtgcagaaa gcagactcct cctttcttta
     1561 tgataagtat cagaatcaga gtattgccgc gcatgtgatg cgcgctctcg ctgctgagca
     1621 gtcggaagtg tcgccagaac agcggcgcgc catctgcgag gcttttgagt ctgccaataa
     1681 tacccatggc ttaaacctga ctgcccataa atacccgggc ttacgcggca cattacaaac
     1741 cgcatccact gactgcgaca caattgtgga agctgcagca ctattacccg cttttgatca
     1801 ggcagtggaa ggcaaccgcc accaggatga ttacggttca ggtcttggga tggccgaaga
     1861 gaaatttcac tattatctcg atctcaatga ccgctatgtc tatttttatg agccggttaa
     1921 tgttgaatac tttgcgatga ataactggtc catcctgcag tcaggaagta ttggcatcga
     1981 tcgcaaagat attgaaaagg tatttaccgg gcgtaccgta ttgtcgagca tttaccagga
     2041 tcagcgtact aaacagaacg tgatgagttt gctgacgccg gtgtatgttg cagggcagct
     2101 aaaagggatt gtgctgctgg atattaacaa aaacaatctg cggaatatct tttacactca
     2161 tgaccgccct ctcctctggc gttttctcaa tgtcacgcta accgatacag attcggggcg
     2221 cgacattatc atcaaccaga gcgaagataa tctgttccag tatgtcagtt acgtccatga
     2281 cttaccgggc ggcattcgtg tctcgttatc cattgatatt ctttacttta tcacgtcttc
     2341 gtggaaaagc gttctgttct ggattttgac ggcgttaatt ttgctgaata tggtgcggat
     2401 gcacttccgc ttataccaaa atgtgtcgcg agaaaatatt agtgatgcga tgactggact
     2461 gtataatcgc aaaattttaa cccctgaact ggagcagcgg ttgcagaaac tggtgcaatc
     2521 cggttcttcg gtgatgttta ttgcaattga catggacaag ttaaagctaa taaatgacac
     2581 cctcggtcat caggaagggg atttagcgat tacgttgtta gctcaggcga ttaaacaatc
     2641 gattcgtaaa agtgattatg ccatccgact cggtggcgat gaattctgca tcattcttgt
     2701 cgattcgacg ccgcaaattg cagcacaact gcctgaacgt atcgaaaaac gtctgcaaca
     2761 tatcgcgccg cagaaagaga tcggcttctc ttccggtatt tacgcgatga aagaaaacga
     2821 tacgttacat gatgcgtata aagcttccga tgagcgttta tatgtcaata agcagaacaa
     2881 aaacagccgt tcatgataac cttctgtggt tgtttgcttg taatctcagg agcgtgaaat
     2941 gactgaaatg gctaaaggaa gcgtgacgca tcagcggtta atcgctttat tatcacaaga
     3001 aggtgctgat ttccgcgtcg ttacccatga agcggtaggt aaatgtgaag cggtgtcaga
     3061 aattcgtggc accgctctag gccagggtgc taaggcactg gtatgtaaag tcaaaggcaa
     3121 tggcgtaaat caacatgttc tggcgatcct cgcagccgat caacaggccg atctgagcca
     3181 actcgccagc catataggcg gattacgcgc ttcgttggcc agcccggcgg aagtcgatga
     3241 actcacaggc tgtgtcttcg gcgcgatccc ccctttcagc ttccatccaa aactcaaact
     3301 ggttgccgac cctctactct tcgaacgatt tgatgaaatc gccttcaatg cagggatgct
     3361 ggataaatcc gttattttga aaaccgcgga ttatttgcgc attgcgcaac cagaactggt
     3421 caatttccgc cgcactgcgt aactagcctg tccgttcaat aagcagaacg gacaaaacca
     3481 gcacaacagc aatggcaaaa aacgatgacg tgataatcag tgtctcgacg aacatttgat
     3541 cgttcatagc atacccccgg gagatagctg ttttcacgtt attgttagcg tgcacaaatg
     3601 gcagtttgat gacagttcgc ctgatttttt atgcaaaaaa cgtaaagatt ttctacttcc
     3661 ttcctgcagc aagcgtaaag taagcaggct tattattttt tggcaaggaa accacgatgt
     3721 ttgatgtcac tttgctgatc ctgctcgggt tagctgcgct gggctttatc agtcataaca
     3781 ccactgtcgc cgtttcaatt ctggtgttga tcattgtccg cgtcacaccg ttaagcacct
     3841 ttttcccgtg gattgaaaaa caggggctaa gtatcgggat aatcattctg acgattggcg
     3901 ttatggcccc catcgccagc gggacgctac cgccctcaac cttgattcac tcgtttctga
     3961 actggaaatc gctggtggca attgcagtag gcgttattgt ctcctggctg ggtgggcgcg
     4021 gcgtgacgtt aatgggcagc cagccgcaac tggttgctgg gctgctggtg ggcactgttt
     4081 taggcgtagc gctgtttcgc ggcgtaccgg ttggcccgct tattgccacg ggtctggttt
     4141 cgctgattgt ggggaaacag tagttaatcc cgcaatatat cggcctggcg tttgccccag
     4201 gcctttctta aacatagtaa taaaggccgt tgttgaatca taccccagcg tatgagcaac
     4261 tttctgtaca gtatcgcctt tcactaatcc ctgaagcgcc ataatcagct gtaactgctg
     4321 tcgccactgg cgaaaactca accccgtctc ttttactatc agtcgggcaa gattgcgttc
     4381 actcatcgca aaaaagccag cccattgccc caatgccccc cactcgacgg gctctttcgc
     4441 catcatctcc accatcgcgc ggattttagg atgagaagaa acgggtaggt gcagctgttg
     4501 ttgtggctgc tgagggagtt catcaaacag gacctgaatt aatctgcggg tcatcggctc
     4561 agcacgctga gtcgtcgtcc tgtttgccag agttaaaatt aattcccggc ataagggcga
     4621 tattttcaat gtgtaacatg ttgtcggcat tgtcactgcg ctgggttcga taaacagaaa
     4681 acagagttca gcgttagctg tcacttgatt gctatgttcc accccgcccg gtatccaaac
     4741 cgcatattgt ggcggcacca tccacaaagc attttccacc gtacaggtaa ttgcaccatg
     4801 tagcgccagt atcagctgtc ctttgcggtg ctgatgtaca ggactggtta actcatccgt
     4861 tccggcgtga atacaaaatg cgaccgcggc ttcatggtgt cggtcgggtt catagccatt
     4921 gagattcaac ctgtgcatca ttttgtccga atttagcgat aatttgtcat tttagcttga
     4981 ttcaacataa caataaaaac ggtaaggtac agcctcgttt gtaacaatga gaagcatatg
     5041 acctgttcaa cttcattaag cggcaaaaac aggattgtcc ttatcgctgg cattctgatg
     5101 attgccacta cattacgcgt caccttcacc ggcgcagcac cgttactcga tacgattcgt
     5161 tccgcttact cgctgacgac tgcgcaaacc ggcttattga ccaccctgcc attattggcc
     5221 tttgcgctaa tctcacctct ggctgccccg gtggctcgac gttttggtat ggaacgtagc
     5281 ctgtttgccg cgttactttt gatctgtgca ggtatcgcaa ttcgctctct cccttcgcct
     5341 tacttattat ttggcggtac agcggtcatt ggcggtggga ttgcattagg taatgtctta
     5401 ctgccaggat taattaaacg cgatttccct cattccgtcg ccagacttac cggcgcatat
     5461 tccctgacaa tgggagctgc ggcggctctg ggatcggcta tggtagtgcc gctggctttg
     5521 aacggttttg gctggcaagg cgcgttgctc atgctgatgt gttttcctct gctggctctt
     5581 tttttatggc tgccacagtg gcgaagccaa caacatgcaa atttgagtac ttcacgcgcc
     5641 ttacatactc ggggtatctg gcgttcgccg cttgcctggc aggtaacatt gtttcttggg
     5701 atcaactcac tggtctatta cgtgattatt ggctggcttc cggcgatcct catcagtcac
     5761 ggctatagcg aagcacaggc gggttcactg catggtttgc tgcaactagc cacagcagct
     5821 cccggtttgc tgatcccact tttcttacat catgtgaaag accagcgtgg tattgcagcg
     5881 ttcgttgcct tgatgtgcgc agtgggcgcg gttgggctct gcttcatgcc agcgcacgcg
     5941 atcacctgga ctctgctttt cggttttggt tccggcgcaa caatgatact ggggttgacg
     6001 ttcattggtc tgcgggctag ttctgcgcat caggcggcgg cactctcggg gatggcacaa
     6061 tccgtcgggt atttgttggc agcctgtggg ccgccgctga tgggtaaaat acacgatgct
     6121 aacggtgact ggtccgtacc actaatgggt gttgcaatac tttcactact gatggcgatt
     6181 ttcggacttt gtgccgggag agacaaagaa attcgctaat atccggtact atagtgacgt
     6241 gacaaatcat gcgtgaaagg gagaacaaac acgatgaata ttcagtgcaa acgcgtttat
     6301 gatccggcag aacagagcga tggttatcgc gtactggtcg accgcctctg gccacgcggt
     6361 atcaaaaaaa ccgatttagc ccttgatgag tgggataaag aaatcacgcc gtcaacggaa
     6421 ctgcgcaaag cctttcacgg cgaagtcgtc gattttgcaa cctttcgcga gcaatatctt
     6481 gcagaactgg cacaacacga gcaagaagga aagcggctgg cggacatcgc caaaaaacag
     6541 ccgctgaccc tgctctactc agcaaaaaac accacgcaga accatgcgct ggtgctggcc
     6601 gactggctac gtagcttgtg attttagtac agcatccggc ggttattttt caccagccgg
     6661 atggtccagt cgccacaatg cccattcatc aatcgtttca ccgcccggta atttgcaatt
     6721 gttgctgacc ccttgcgctg tctgcactgg aatgagcgtc ccgcccttct gctggcaata
     6781 gaccgacgcc ggatttgcca taccaatctg cggcggttta ggtgcttctg gctgagaagg
     6841 ggttgaacaa ccagccagga ccagcaagca aggcagaaca aaactgataa ttttcattta
     6901 ttgatctcac atatgtatcc aagcttagag tatcgcggta tcgttttgtt ttgcagcact
     6961 atttttatta cattcactca aaacatatta cgtcttgttt catctttgtt gatgatgttt
     7021 tatcatgcct gcaaagatta aataatcagc atttacccgc cgtatcctgg agttgtttcg
     7081 tgtcagatca gattatcgcc cgcatctcgc aatcccttgc caaagaacag tcactggaaa
     7141 gtctggtccg acagcttctg gagatgctgg aaatggtcac tgatatggaa tcaacctacc
     7201 tgaccaaagt ggatgtcgaa gcgcgcctgc agcatattat gtttgcccgt aacagccaga
     7261 aaatgcacat cccggagaat tttaccgtct cgtgggatta ctcgttatgc aaacgcgcca
     7321 ttgatgaaaa ctgctttttc agcgatgaag tccctgaccg ttggggcgac tgtattgcgg
     7381 cacgcaatct tggcatcacc acatttctga gcacgccaat tcacttaccg gatggatcat
     7441 tctatggcac gctttgcgcc gccagcagtg agaagcgcca gtggagtgaa cgcgcggaac
     7501 aggttttaca gttattcgcc ggactgattg cacaatatat tcaaaaagag gcactggttg
     7561 aacagctgcg cgaagccaat gctgcgctga ttgcgcaatc gtataccgac tcgttaaccg
     7621 ggctaccgaa tcggcgggcg atttttgaaa atctgacgac gctgttttcc ctcgcccggc
     7681 atcttaacca aaagataatg atcgcgttta tcgatctgga taacttcaaa ttaatcaatg
     7741 atcgttttgg tcataatagt ggcgatctgt ttctcattca ggttggcgag cgccttaata
     7801 cgctccagca aaatggcgaa gttattggtc gtctcggcgg tgatgagttt ttggttgttt
     7861 cactaaacaa cgagaatgcg gatatttcgt cgctgcgaga acgcattcag cagcaaatac
     7921 gcggagaata tcacttaggt gatgttgatt tgtattatcc tggtgccagt cttggcatag
     7981 tagaagtcga tcctgaaacg accgatgcag acagtgccct acatgctgcc gatatcgcga
     8041 tgtatcagga gaaaaaacac aaacagaaaa caccttttgt cgcgcattca gcgccacatt
     8101 cctgaggcgt attcacatcc ttttgattgg tgataacatg cgaatcggta ttatttttcc
     8161 ggttgtaatc ttcattacag cggtcgtatt tttagcatgg ttttttattg gcggctatgc
     8221 tgccccggga gcataaagat gaaaaaaaca acgatcatta tgatgggtgt ggcgattatt
     8281 gtcgtactcg gcactgagct gggatggtgg taacgtcaca tctaaaaaat agcaaaggct
     8341 gcctgtgtgc agcctttgtg caatttacgc gttaactttt aatcttcctg tagataaata
     8401 gcacgacaat cgcaccaata acggcaacca cgaagctgcc aaaattgaag ccatcgactt
     8461 taccaaagcc aaacagcgtg ctgatccaac cgccgactac ggcaccgact atccccagca
     8521 ggatagtcat aaagaatcca ccaccatctt tacctggcat gatccacttc gccagaatac
     8581 cggcaataag cccaaaaata atccatgaca gaatgcccat tgtttcctca cttatctgtt
     8641 ttgcattagc gggttagtcg ctgataaaaa gcatagcaca acatcgggag ggcaagattt
     8701 gtgacgagca tcacggaggt ttttttgcga tggcgcagaa attgcgccat caacgatcag
     8761 tggtaattac caaccacaaa catcatgttc gttttccgtg tcataagaac gcacggtatt
     8821 caccagatct tttatcactt cagccgccac ttctggcacc agcaaagtca tcggcgtctc
     8881 tgtttcataa tcgacagaaa cgccattgct gttattggtg acggtcacgg tatacgttgc
     8941 tttgcccatg attcatttcc cgttatgaat gactttccgt tgttgcgcac cttccatcag
     9001 gacttcagga gccacgaaga agtcaatgtt gaaataagta tcgtcagtca tggcttcaat
     9061 gttgtgccac ttttctggag ggaacaccgc aaactgcccc gcttcgataa ggatcacctg
     9121 atcaggctct gcactgtgtt catcagcgta gccgagatat ttgaccgccc catgcataac
     9181 ggaaaggcgt gggtaaaccc ccgggcgcgt tcctttatca agatgacgtt cgaatattcc
     9241 ggcaggcgca gtttgtttat tccagaaagg cgttgagcgc gtatgaatat aattctgtgg
     9301 gatttgcagc atccttttcc ctccttcggt gaatgcgctg aaaacggttt attccagccg
     9361 tttcagggta cgcctgataa tttgcttttt aaataccatt tattggttac tttttagcac
     9421 catatcagag aaaaatcagg gaggattata gatgggaagc ccgcgacaac gcgggctgaa
     9481 agcatcagga ttgcagcgtc gccagtcggg cagcgaaacc cacgaacatc aaaccaatca
     9541 gtgagttgcc cactttagcc agtttctttt tggtacgtat gtactgcgtg acaaaagcac
     9601 cagatataat caggaagctc aaatagcaga aactcaccag ttccagcgtc gtcgccagaa
     9661 taaagaatga aattcccgta tgtggggcat taacatcgat aaactgtacg aaaaacgaca
     9721 catagaacaa aatggctttc ggattagtca ggctcaaaat taacgcgcgt ttaaaaatag
     9781 caccgtattg gggctcatcg gatttggcct cgttattttt acccttcagg gtcgcgtaaa
     9841 gaattttact ccccagatag agcaaataaa acgcaccaag ataacgtaca atgttgaata
     9901 atatcggggt ggtcttaatt aatgtcgcca ttccagccca tgccagaaac atcaataccg
     9961 catcgccaat aaatacaccg caggccgcaa gataaccgcc tttcataccg ctactgacgc
    10021 tatttttgag tacaaacagg gtatttggcc ctggcaccaa cacaataaaa atggccccaa
    10081 ccagataggt ccagtaattc agaaccccgt attcagcgaa cacattaacc tctttaatta
    10141 tctttcgatc atgcgcgatt aaaggtgaat atgctaacca atctgtagcg gcttagaaag
    10201 gagaaaatca ggttttaacc tgatatcaac ccgataattg aatcattaat caggcatgct
    10261 ccagtgaaaa attcgggtag tgctcggcaa aatactggcg taaaaattct accgttatcc
    10321 gcactttcgc tgacgtcgcc agccttgaaa cataaacggc ccagacgttc gctggctggt
    10381 aatattccgg tagcacttgc actaaatgac cactggcaat gttttcgcta acatcccacc
    10441 aggaacgcag agcaatcccc tgcccatcga gacaccactg atggacaatt tctccatgat
    10501 tagacgacag tgggccggtg actttaatcg catggtggcc ttctttattg cgtaattgcc
    10561 agacaccaaa aggatgatcg cgctctttaa tgaccaggca cggcaatgca gaaagatcgg
    10621 ttaaatgttt cggcgcgcca tgttgcgcaa taaattccgg cgacgcgcag agaatgcggt
    10681 aattggtcgc cagtttgcgg gcgattaaat taggggcgat gtcatcgcca atgcgaatat
    10741 cgagatcgac accttcattg accaaatcga ccagccggtc ttccacatca aaacgtaatt
    10801 ccagttgcgg atacgccttc gccagcgctg ataacgccgg agccaccacc tgtcgtccaa
    10861 aaccaaagct gctgataata cgcagcatcc cctgtggcac ctgacgcacg tcagaaagtt
    10921 cgtccatcat ctgaccgaca tcctgcaaaa tccgctgcgc ccattcataa atccgctctc
    10981 cttcttcggt aatggtgacg cggcgggtgg tgcggtgtaa caacaccacg tttagcgttt
    11041 gctccagcaa ggcgatgcgc ttgctgacga acgccggtga aacgcccagt tcttccgcca
    11101 cggcggcaaa accagcccgg cgagccacca gcataaagac gcgcaaatca ttcagcagcg
    11161 gtaaattatt catgattcgt gttttatgtt tcaccagtta cggggattaa ttccttttca
    11221 gtcaattata ggatggtgat gttgtcaatt ttgatggtca ggaagtgaga acccaatgat
    11281 gaaaacgatg cgtattgctg cgatcccggg agacgggatt ggcaaagaag tccttcctga
    11341 agggattcgc gtgttacagg ctgccgctga gcgctggggc ttcgccttga gttttgagca
    11401 aatggagtgg gcgagctgcg agtattacag ccatcacggt aaaatgatgc cggacgactg
    11461 gcatgagcaa cttagccgtt tcgacgccat ctattttggt gccgtcggct ggccggacac
    11521 cgttccggac catatttcgt tgtggggttc gctgctgaaa tttcgtcgtg aattcgacca
    11581 gtacgtcaac ctgcgcccgg ttcgtctctt tcctggcgtt ccctgcccgc tggcgggaaa
    11641 acagcctggc gacatcgatt tttacgtggt cagggaaaac accgaaggcg aatattcctc
    11701 gctcggcggt agagtgaatg aaggtacaga gcatgaagtc gtcattcagg aatcggtatt
    11761 tacgcgtcgt ggtgtcgatc gcattttgcg ttatgccttc gaacttgcgc aaagccgccc
    11821 acgtaagacg ctaacttctg ccactaaatc aaacggttta gccatcagca tgccgtactg
    11881 ggatgagcga gtggaagcaa tggccgagaa ttacccggag atccgctggg acaagcagca
    11941 tattgatatt ctctgcgcgc gttttgtgat gcagccggaa cgcttcgatg tggtggtggc
    12001 gtccaatttg tttggcgata tcctttccga tcttggcccg gcctgcaccg gcaccattgg
    12061 cattgcccca tccgccaacc tgaatccgga acgcactttc ccctcgctct tcgagcctgt
    12121 ccacggttcc gcgccggata tctacgggaa aaatattgct aaccctatcg ccacaatttg
    12181 ggccggggca atgatgctcg attttctcgg caatggcgat gagcgtttcc agcaagcgca
    12241 taacggtatt ctggcagcga ttgaagaagt gattgctcac gggccgaaaa caccggatat
    12301 gaaaggcagt gccaccacgc cacaggttgc cgacgcgatt tgcaaaatta ttttgcgtta
    12361 aggtcaaacc agtttatttg aaccgcgtca ctgacgcggt tttgttattc gttctttgca
    12421 gttaataacc tgcgtcattt caccttttat tgtttccgtt tcgtgtttta tggctttccg
    12481 tattcttaat tgtttaattt atgtaacatg caaataatca attcgttaaa cacgacatac
    12541 aactcattac cctgcaaata attacaactg gcaaaagcca gagacgggct tttatgccca
    12601 ggagatgact catgatgagc aatgttaaga aaaaagatgt gccgctgata agcatcagcc
    12661 tggtggccat tcttttcatc gcagctgcat taagcctttt cccacaacaa tcggccgacg
    12721 cggccaacgc aatatacact tttgttacgc gtacgttagg ttccgcagta caggtattgg
    12781 ttttgctggc aatgggacta gtgatttatt tagccaccag taaatacggc aatattcgtc
    12841 ttggcgaagg aaaaccggaa tacagcacgc tctcctggct gtttatgttt atttgtgccg
    12901 gtttaggttc ttctacgctt tattgggggg ttgctgaatg ggcctattat tatcaaacgc
    12961 ctggattaaa tatcgcaccg cgttcacaac aggcactcga atttagcgtc ccttactcct
    13021 tcttccactg gggcatcagc gcctgggcaa cttatacgct ggcctcatta atcatggctt
    13081 atcactttca tgtgcggaaa aacaaaggtc tgagtctttc cggcattatt gccgcgatta
    13141 ccggcgttcg cccgcaaggc ccatggggaa aactggttga tttgatgttc ctgatcgcca
    13201 ctgtcggcgc actgactatt tcccttgttg tcaccgcagc aacctttact cgtggacttt
    13261 ccgcactgac cggtttaccc gataacttca ccgtgcaggc atttgtgatc ctgctttccg
    13321 gcggcatttt ttgcctaagc tcatggattg gtatcaacaa cggtttgcaa cgtctgagca
    13381 aaatggttgg ctggggcgcg ttcctgctgc cattactggt gctgattgtc ggtccaaccg
    13441 aatttattac caacagcatc atcaatgcca tcggcctgac cacgcaaaac ttcctgcaaa
    13501 tgagcttatt caccgatccg cttggcgatg gttcatttac ccgcaactgg accgttttct
    13561 actggctgtg gtggatctca tacacccctg gcgtagcaat gtttgtcacc cgcgtttccc
    13621 gcggtcgtaa gattaaagaa gttatctggg gactgatcct cggcagcacc gtcggttgct
    13681 ggttcttctt cggcgtaatg gaaagctatg ccattcatca gtttatcaat ggcgtaatca
    13741 acgtcccaca ggtgctggaa actctgggcg gcgaaacagc tgtacagcaa gttctgatgt
    13801 cgttgccagc cggtaaattg ttcctcgccg catacctggg cgtgatgatt attttccttg
    13861 cctcgcatat ggatgcagtg gcctacacca tggcggcgac cagtacgcgt aatctccagg
    13921 aaggtgacga tcctgaccgt gggctgcgtc ttttctggtg cgtggtgatc actctgatcc
    13981 cgctttccat cttgtttacc ggtgcttcgc tggaaacgat gaaaaccacc gtcgtgctca
    14041 cagcccttcc cttcctcgtc attttactgg tgaaagtcgg cggatttatt cgctggctga
    14101 aacaggatta cgccgacatt ccggctcatc aagttgaaca ttatctcccg cagacaccgg
    14161 ttgaagccct ggaaaaaaca ccagtgctcc ctgcgggaac cgtattcaaa ggcgacaact
    14221 gagcgccatc atcctaacga taaaggtatc cctatgagca atctgagccc tgactttgta
    14281 ctacccgaaa atttttgcgc taacccgcaa gaggcgtgga ccattcctgc ccgtttttat
    14341 accgatcaga acgcctttga acacgaaaaa gagaacgtct tcgccaaaag ctggatttgc
    14401 gtcgctcaca gcagcgaact ggcgaatgcc aatgattatg tgacgcgtga gatcattggc
    14461 gaaagcatcg tgctggtacg cggtcgtgat aaggttttgc gcgcgttcta taacgtgtgt
    14521 ccgcaccgtg gtcatcagtt gttgagcggt gaaggaaaag caaaaaatgt gattacctgc
    14581 ccatatcacg catgggcatt caaactcgat ggcaatctgg cccatgcacg taactgcgaa
    14641 aacgtcgcca atttcgatag cgacaaagcg caactggttc cggtgcgtct ggaagaatat
    14701 gccggattcg tcttcatcaa catggacccc aacgccacca gcgtagaaga tcagttaccc
    14761 ggtctgggcg cgaaagtgct ggaagcctgc ccggaagtcc acgatctgaa actggcggcc
    14821 cgctttacca cccgcacgcc tgccaactgg aagaacattg tcgataacta tctcgagtgc
    14881 tatcactgtg gtccggcgca tccaggtttc tccgactccg tacaggttga tcgttactgg
    14941 cacaccatgc acggtaactg gacgctgcaa tacggtttcg ccaaaccgtc cgaacagtcg
    15001 tttaaatttg aagagggtac ggatgcggca ttccacggtt tctggctgtg gccgtgcacg
    15061 atgctgaatg tcaccccgat caaagggatg atgacggtca tttatgaatt cccggtggat
    15121 tctgaaacta ccctgcaaaa ctacgatatt tacttcacca atgaagagtt aaccgacgag
    15181 caaaaatcgc tgattgagtg gtatcgcgat gtgttccgtc cggaagattt acgtctggtt
    15241 gaaagcgtac agaaagggct gaaatcgcgt ggctatcgtg gtcaggggcg catcatggcc
    15301 gacagtagcg gtagcggcat ttccgaacat ggtatcgccc atttccataa tctgctggcg
    15361 caggtattta aggactaatg acatcggcgg cggtattttc cgccgccggg ctgatttttg
    15421 atggagtaca gcaatgtcag actatcaaat gtttgaagta caggtgagtc aggtggaacc
    15481 gcttaccgaa caggtgaaac gcttcacgcc ggtggcaacc gatggcaaac cgctgcctgc
    15541 gttcaccgga ggaagtcaca tcatagtgca gatgagcgat ggtgataacc agtacagtaa
    15601 tgcgtattca ctactgagtt caccgcataa cacctctagt tatcagattg ccgttcgact
    15661 ggaagaaaac tcgcgcggcg gttcccgctt tttgcatcag caggtaaaag tgggcgatcg
    15721 gttaacgatc tcaacgccta ataacctgtt tgcgctaatt ccctcagcca gaaagcatct
    15781 gtttatcgcg ggcggtattg gtatcacccc tttcctgtcg cacatggcag agttgcaaca
    15841 cagcgacgtc gactggcagc tacattactg ctcgcgaaat ccagaaagtt gcgcatttcg
    15901 tgatgagcta gtccagcatc cgcaggctga gaaagtccat ttgcatcatt catcaaccgg
    15961 aacacgactg gaattagcgc gattattggc ggatatcgaa cctggcacac acgtttatac
    16021 ctgtggcccc gaggcgctaa ttgaagcggt aagaagtgaa gctgcgcgtc tggacatcgc
    16081 tgccgatacg ctgcactttg agcaatttgc tatcgaagac aaaaccggcg atgcatttac
    16141 cctggtgctt gcccgttccg gaaaagagtt tgtggtgccg gaagagatga ctattttgca
    16201 ggttattgaa aataataaag ccgcgaaagt ggaatgttta tgtcgtgaag gggtatgcgg
    16261 aacctgcgaa acggcaatac tggaaggtga agctgaccat cgggatcagt attttagcga
    16321 tgaagagcgt gccagccagc aaagtatgtt gatctgttgt tcgcgtgcga agggtaaacg
    16381 cctggtgttg gatttgtagt ttgccgaagc cggatgtggc gctgagcgcg cccagtccgg
    16441 cttcggaaaa ttttactgcg gatattcctg caataaattg tgtaatgctt ccgccatcag
    16501 ctcaccacgc cagccggaaa tcagctccgg caaattgttc tgcggtttca gtttccagtg
    16561 ccagttcagc agttggttga tttgccgacg cgatgccagc aattcggcgc tgatcttgtg
    16621 cgtttcgctc acgtcagtaa tcagcgactt aatcgcttta aacgctttac gataacccgg
    16681 catgtccatc aggttaagca tcggctgcgg taaggcctct tccggcaatg tctgcgcttt
    16741 ttccaccagc gccagcagcg ttttaccgtg aaagcggatt tcgctaccgg ataaacccag
    16801 gctgtccagt tcgcctaaac ttcccggcat ataacgcgct accgaccaca aatgctcttc
    16861 acgcacgaca aagttcaccg ccagatcgcg ctctcgcgcc ttgcgcagtc gccagtcggc
    16921 taacagttgc agacaggcca gttggcgtgt gcgtaattgc caggcattgg tgatatcacg
    16981 ccaggcatct tccggcgcaa cgacttcctg acgacgcatt tgcatcaggc gacattcatc
    17041 cagcgccgct ggtagccagc cggaggcctc cgtttctacc ataagcttgg cggtgatcgg
    17101 taacagatac cagacatccg ccgctgcata ttcacactga cgttcggtca gcggtctggc
    17161 cagccagtcg gtgcgcgatt cactcttgtc cagcgtaacg ccggaatact cttccaccat
    17221 ggaagcgaaa ccccatgaca tcgggcgtcc gcagaaggca gcaaggattt gcgtgtcaat
    17281 caggggttgt ggtaattcgc caaagacatt gaggaacact tccagatctt cactgcctgc
    17341 atggagaaat tttgtgatgg acggatcgcg caggatcgct ttcagcggtg accagtcggt
    17401 gatcccgaga ggatcaatta gcgccagatg ctcgccatcg aaaagttgaa tcaaccccag
    17461 ctgcgggtaa taagtgcgcg tacgaacaaa ttcagtatcc agggctatcg ccggaaaggc
    17521 acggacggct tcacacaaag aagccagcgc atcgtccgtg gtaatcattt ggtaattcaa
    17581 attgttttct ctttagtggg cgtcaaaaaa aacgccggat taaccggcgt ctgacgactg
    17641 acttaacgct caggctttat tgtccacttt gccgcgcgct tcgtcacgta attctcgtcg
    17701 caaaattttt ccgacgttag atttcggtaa ctcatcacga aactccacca gcttcggtac
    17761 tttgtagccc gtgagctgac ggcggcaaaa ggtcaccagt gactcttcgg taagcgatgg
    17821 atcttttttc actacgaaga ttttcaccgc ttcaccactg gagccggaag gtacaccaac
    17881 agccgcgact tcctgtacgc caggatgctg catgacgaca tcttcaatct cgttgggata
    17941 gacgttaaaa ccggaaacca aaatcatgtc ttttttacga tcgacaatgc gcaggaatcc
    18001 ttcttcatcc attaccgcga tgtcgccagt gtgtaaccag ccgtttttga tgatttcatc
    18061 ggtagcatcc ggacgctgcc aatagcccag catcacctgt ggacctctga cgcaaagttc
    18121 acctggctga cctggcgata cttcattatc gtcgtcatcc accagtttgg cttccgtcga
    18181 cggcaccggc aaaccaatgc taccgctatg ataatcaata tcataggggt tcacgctgac
    18241 cagcggcgca cattcggtca ggccataacc ttccagcaga tactgtccgg tcagtttcac
    18301 ccagcgttct gccaccactt gctgaacagg catcccaccg cctgcagaaa ggtgcagact
    18361 ggagaaatcc agttgttgaa actctttatt gttcagcaac gcgttgaaca aggtgtttac
    18421 gccggtgata gcggtaaacg gatatttcgc cagctctttg accagccccg gaatatcccg
    18481 cgggttagtg ataagcaggt tctgcccacc cagttcgata aacagcaggc agttaatggt
    18541 cagtgcaaaa atgtgataca gcggcagcgc cgtcaccacc agctctttgc ccggatgcag
    18601 cagaggccca tacgtagcgt taacttgttc caggttcgcc agcatattgc ggtgagtcag
    18661 catcgcgcct ttcgccacac ctgtggtgcc gccagtgtat tgcaggaagg ctaaatcttc
    18721 tggcaccagt tcaggtttga catactgcat acggtagccg ttgtgcaggg cgctacgaaa
    18781 tgaaatggca tctggcagat gatatttcgg caccagacgt ttgatgtatt taacaacgaa
    18841 gttaaccagt gtgcctttcg ctgtggaaag ctgatcgccc atacgggtca ggataacgtg
    18901 ctgaactgcg gttttatcga ccactttttc cagcgtgtga gcaaagttgg acacaatgac
    18961 aatcgccgat gcgccgctat cgttaagctg atgctcaagc tcacgcgggg tatacaacgg
    19021 gttaacgttt acgacgatca tcccggcacg caaaatgcca aacagcgcca ccggatattg
    19081 caataaatta ggcatcatca acgcaacgcg atcgcctttc ttcagcccca acccttgttg
    19141 caaataagcg gcaaacgcgc gactgcgttc ttccagcttg cggaaggtca tcacctcccc
    19201 catattcaca aacgcaggct gatcggcgta gcgcgcgacc gactgctcaa acatatctac
    19261 cagagattga taacggtcag ggttgatctc cgtcggaacg tccgcgggat aacggttaag
    19321 ccaaaccttc ttcaattctt cacctctaaa atgcgtgttc gtcgtcatcg caaccccaaa
    19381 tgatatacat gccgttaaca taatattaac tcatcatacc agcttgataa ttacccaacg
    19441 aaaaggttgc gaagcgcgtc actatttatt tttatcttta ccgtaagaat gcagaaacag
    19501 cggaccagcc gctgtttctt tttctttaaa aacaagcaat tattcagtta caactgtttg
    19561 tactctcgcg gggccgggat tataccagcc ccatccgccg tggccatagg gccagccacg
    19621 accgccataa aaccagggat caatcggctg aggcggcata atcacctgct gggttaaatg
    19681 ccaacgtttg taacctgttg cctgcatcac cataaattta tagggcgtgt tgccgatttt
    19741 tccgtcaacc gcaccagtga ttggcccgac taccgtaacc agttgtccac ggaaatccac
    19801 cgggtccaga aaaccgttca catcggcata aatgcgaccg cgagaaggtt ctcccagcgt
    19861 cggtctggct ccgctgtcca gcggtacggt agcaatttcc aggcgggttt tcccttgctg
    19921 gttttgtacc gcaaccactt tgccaccaaa gcgtgcctcc tgaccaacgt acagctgcgg
    19981 cgcactcatc acccgaacta aatcttgttg cggcgtggga ctgctgcctt taatggcgtc
    20041 cggcacagtg acacaaccgc tcagcattag cgcaaacgta cctgccagta tgcctttgat
    20101 aacattcttt tgaaccgcca tgatgcgact cctttttttc tcagggcata ctcttaagat
    20161 tcattctttg cccggaagtt tcttccatgc gacgttgtta cgtaaataaa ccggttcggc
    20221 atgttccacc gccaccgttt taccctcggc aaacatctga caagcaatcg gcagcatatc
    20281 ttctgcagcg ggcagtaaaa cttctccgtc gcgcaaaacc agtccgctct ctttaccgag
    20341 atccggccag gcttgccagc ctgtacctac cgtcacccat tccccggaaa gctgttgcat
    20401 tcgttcatga acgagttcgg gtttgagtac ggcttcggtt tcttcaccgt gccagatacc
    20461 gttttcatca cgctgatatt cggcccagta aacttcgccc attcgcgcgt caatggctga
    20521 cagcacgcgt gttgcgccgt ttttgcgcca cgccccttgc gccatcgtca ttagcgtgga
    20581 gacgccaatc atcggtaatt ccgcgccaag cgccagccct tgcgcgatgc caataccaat
    20641 gcgcacgcct gtaaagctac cggggccgcg cccgtaagcc agagcgttaa tatcagtcag
    20701 ggaagttccg ctggtggtca ggatatcctg caccatcggt aagattcgtt gagtatgttc
    20761 acgagggcaa agctcaaaat gagcgttgac agtaccgtcg ttccacaggg cgacagagca
    20821 ggcctctgtc gcggtatcga tagccagaat tcgcatgggt cttcgtgctt agatcaataa
    20881 aaaggcgcgc atcataccat actccgtaac aaattacctg gaggatggta tcgcaaggaa
    20941 acgaaccgca cgggcaatgt cacgggtgcg tggcgcgggc ggcagactgg cgagaaacgt
    21001 cgcgccgtaa ggacgcatca ccagccgatt gtcacaaatt accaaaaccc cgcgatcgtc
    21061 ggcgtcgcga atcagtcgcc cgactccctg cttgagagta atcaccgcat ccggcagttg
    21121 tacttcatcg aacgggtcgc caccgcgcaa acggcaatct tccatgcgcg cttttaacaa
    21181 tggatcatca ggcgaggtaa acggcaattt gtcgataatc accaatgaca atgtatcgcc
    21241 acgcacgtcc accccctccc agaaactact ggttgccaca agaagcgcgt taccggcact
    21301 gacaaattgc tgcaacagtt gccctttgct ggtttccccc tgcaataata cgggaagcgt
    21361 catggtagcg cggaactgct cggccagatc gcgcatcatg gcgtgcgagg tacaaagcat
    21421 aaaacagcga ccgttgttag cttcgatgat cgggcgcagc atagccgcca gttgccgcgc
    21481 ggaacccggt tgattggttt gcggcaggtt gcgcggcaca cagagtaacg cctggcggct
    21541 gtaatcaaac gggcttggca atagcaacga ctccgcctgt tctatcccaa gccgcgaggt
    21601 gaaatgatgc agatcgtcgt ttaccgacag cgttgctgag gtgaagatcc agctaccggg
    21661 cttttgcgcc attaactctt tgaatttatc cgccacgctg agcggcgtga gagccagagt
    21721 aaaatggcgc gaagtgcatt cgtaccagta gctgtagccc ggctgattga tctcttttag
    21781 ccgcttcagc cgtgtgcgat acaacgtggc gcgctcaaat gccgcatcca gcaaggcgga
    21841 acgccccagt gacagtttcg ccacgtcata acaaagttcc agggtgtcat cgagcagtaa
    21901 aaatgcccgc tgaatttgcg gattagctaa cagctcacgc aggttaccgc gataacctgg
    21961 ttcaccgagt tgcagacgaa aatcctgcgc actctgggca agacgatcag cgcacttttg
    22021 taactgctgg gtgtctttta attcggtgcg gtaggcgatg gtgatgtctt ttgccaggtc
    22081 gagcagttgt cgactggaga gtgactgacc aaaatactgg ctggcaatgt ccggtagctg
    22141 gtgggcttcg tcgaagatca tgacgtccgc ttccgggatc aactcgccaa atccactctc
    22201 tttaaccacc atatccgcca gaaagagatg atggtttacc accaccacat cggcgtccat
    22261 cgctttttta cgtgctttga ccacaaagca atctttatac atcgggcagt cgctgccaag
    22321 acagttgtcg ttggtgctgg tgaccagcgg ccacgcctgc gaatcttccg ccacgctgac
    22381 gcaggtgctg atatcaccat cgactgtttg attagaccag gagcgcagca ggatcacatc
    22441 gcttaatatt tgtaccggaa gatcgccccc cgccagcgcc tgctgttcga gacgttcgag
    22501 gcagaggtag tttgagcgcc ctttcagcaa cgccacgttg cccgtgtatt tcaacgcctt
    22561 tgagaccgtc ggcaaatcgc ggctatagag ctgatcctgc aacgcttttg agccggtcga
    22621 gataatgact ttctttttcg cccgcagcgc aggggccagg taagcgtagg ttttgcccgt
    22681 accggttcct gcttccacca ccagcggctg gcctttttct atcgcctggg tgacggctac
    22741 cgccatctgt cgctgtggtt ctcgcggctt aaagcctggt atcgctttcg ccagctgacc
    22801 gtctggtgca aaatcgtccg tcacactacc ccctgttgat ttgaacaggg attatgtcag
    22861 gatgagggcg ctttcgccag ttgaagtggt gacggcgacc tcacattgtg gcagtctttg
    22921 cagcacgaaa atggaaggat aatgaggata aaatgatgac tatcgttcgt atcgatgctg
    22981 aagcccgctg gtctgatgta gtaatccaca acaacacgct ctactacact ggtgtaccgg
    23041 aaaacctcga cgccgatgcc tttgagcaaa ccgccaacac cctggcgcag attgacgccg
    23101 tgctggaaaa acagggcagc aataaatcga gcattctgga tgccaccatt ttcctggccg
    23161 ataaaaacga cttcgcggcg atgaataaag cgtgggatgc ctgggttgtc gcgggtcatg
    23221 cgccggtgcg ctgcacggta caagcgggtt tgatgaaccc gaagtataaa gttgaaatta
    23281 agattgtggc tgcggtgtaa gctttatcga agcaaaataa gtcagacgat aatttatcga
    23341 taatactggt cggttataca t
//
LOCUS       DAZKIP010000069        22737 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZKIP010000069 DAZKIP010000000
VERSION     DAZKIP010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22737
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.069"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            361..765
                     /gene="sdhC"
                     /locus_tag="ACHOUK_002918"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             361..765
                     /gene="sdhC"
                     /locus_tag="ACHOUK_002918"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFP9898323.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            759..1106
                     /gene="sdhD"
                     /locus_tag="ACHOUK_002919"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             759..1106
                     /gene="sdhD"
                     /locus_tag="ACHOUK_002919"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFP9898324.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFCASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            1106..2872
                     /gene="sdhA"
                     /locus_tag="ACHOUK_002920"
     CDS             1106..2872
                     /gene="sdhA"
                     /locus_tag="ACHOUK_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFP9898325.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            2888..3604
                     /gene="sdhB"
                     /locus_tag="ACHOUK_002921"
     CDS             2888..3604
                     /gene="sdhB"
                     /locus_tag="ACHOUK_002921"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFP9898326.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            3905..6706
                     /gene="sucA"
                     /locus_tag="ACHOUK_002922"
     CDS             3905..6706
                     /gene="sucA"
                     /locus_tag="ACHOUK_002922"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFP9898327.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            6721..7938
                     /gene="odhB"
                     /locus_tag="ACHOUK_002923"
     CDS             6721..7938
                     /gene="odhB"
                     /locus_tag="ACHOUK_002923"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFP9898328.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            8032..9198
                     /gene="sucC"
                     /locus_tag="ACHOUK_002924"
     CDS             8032..9198
                     /gene="sucC"
                     /locus_tag="ACHOUK_002924"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFP9898329.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            9198..10067
                     /gene="sucD"
                     /locus_tag="ACHOUK_002925"
     CDS             9198..10067
                     /gene="sucD"
                     /locus_tag="ACHOUK_002925"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFP9898330.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            10734..11639
                     /locus_tag="ACHOUK_002926"
     CDS             10734..11639
                     /locus_tag="ACHOUK_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9898331.1"
                     /translation="MRGKIPKTELLVTFEVVARHESYTRAAEELALTQSAVFRQVSAL
                     EEFLRTPLFSHSKKRIFLNDTGKYYLGIVKETLNKLERDTNTIMTWQPTVQVIELAVN
                     PTFSTHWLIPNLHEFTKLHPDIIVNIHSLANNGDFLNREYDAVIMRENFCAPWAEVEY
                     LFEEEILPVCSGSLLAMSDQKLSVAELLTELPLLHQSTRITGWEEWFALSGVSSPLVN
                     NGPRFDLLSMLIAAVRSNLGVALLPRFAIQHDLDSGDMVIPCDVPIRTGNRFIMTWQE
                     EKSDSPHLQQFREWLLAKSVVPQEM"
     gene            complement(11673..12275)
                     /gene="cobO"
                     /locus_tag="ACHOUK_002927"
     CDS             complement(11673..12275)
                     /gene="cobO"
                     /locus_tag="ACHOUK_002927"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308783.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFP9898332.1"
                     /translation="MEARISTERHVQRQQKLKEQVDTRVAAAIEKKGILIVFTGNGKG
                     KSTAAFGTVTRAVGHGKTVGVAQYIKGQWDNGEYNLLQPLGVEFHIMGTGFTWETQNR
                     QADIDAAKEVWSESKRMLADKRYDLVVLDELTYMLAYHYLDTEEVIASLQNRPAQQSV
                     IVTGRGCHSQILKMADTVSEIRPVKHAFDNGIQAQPGIDW"
     gene            complement(12285..13937)
                     /locus_tag="ACHOUK_002928"
     CDS             complement(12285..13937)
                     /locus_tag="ACHOUK_002928"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFP9898333.1"
                     /translation="MSKPFIWQEPFLQNKDGTEYTLISDQHITVTELDGEEVIKIAPE
                     ALTLLARQAFYEASFFLRSAHLQQVASILNDQQASSNDKYVALQLLRNAEVSAKGVLP
                     NCQDTGTATIVASKGQQIWTGGNDAEALSKGIYSTFQENNLRFSQNAPLDMYTEVNTQ
                     TNLPAQIDISAVAGDEYHFLCVNKGGGSANKAALYQETKSLLQPEKLTAFLIEKMKSL
                     GTAACPPYHIAFVVGGLSADQTLKIAKLASTKYYDNLPTSGNEQGQAFRDIELEKVLL
                     EASQQFGIGAQFGGKYFAHDIRVIRLPRHGGSCPIAMALSCSADRNIKAKINKHGIWL
                     EKLEHNPGQYIPASLREENHAQHVQLDLNRPLRDVMQDLARLPVGTRVSLSGPIVVAR
                     DIAHAKIKARLDSGEPMPEYLKHHIVYYAGPAKTPENMACGSLGPTTGGRMDGYIDTF
                     QAAGGSLVMLSKGNRSQQVTDACHKHGGFNLGSIGGAAALLAQEYVKSLRCLEYPELG
                     MEAVWMMEVENLPAFILVDDKGNNFFSQFEQQHRCASCPAGH"
     gene            complement(14045..15325)
                     /locus_tag="ACHOUK_002929"
     CDS             complement(14045..15325)
                     /locus_tag="ACHOUK_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308785.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HFP9898334.1"
                     /translation="MKKISLTTMILLALVLGMIIGVVLNNTASPETAKLYAQEISIFT
                     TIFLRLIKMIIAPLVVSTLVVGIAKMGDAKALGRIFSKTLFLFICASLLSIALGLITV
                     NFFMPGTGINFVAHGAETTGVVAAEPFTLKVFISHAFPTSIVDAMAHNEILQIVVFSI
                     FLGCSLTAIGEKGSAIVHALDSLAHAMLKLTGYVMLFAPLTVFAAISALIAERGLAVM
                     VSAGIFMGEFYFTMLLLWVLLIGLAIVYVGPCIRRLTRALSEPALLAFTTSSSEAAFP
                     GTLEKLEQFGVSPKIASFVLPIGYSFNLVGSMAYCSFATVFIAQACNIHLSIGEQITM
                     LLILMLTSKGMAGVPRASMVVIAATLNQFNIPEAGLILLMGVDPFLDMGRSATNVMSN
                     AMGAAMVSRWEGEHFGEGCRGKALKPNESNVALP"
     gene            complement(15486..15803)
                     /locus_tag="ACHOUK_002930"
     CDS             complement(15486..15803)
                     /locus_tag="ACHOUK_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HFP9898335.1"
                     /translation="MKQSIVSLAQVIRSKNAGPYELVLDILFKTKENYERVKSSGQLT
                     PELIARLYHVEPDFIHRIVWFDPSNAVKIVMPRDIISGNVGDNDVYGAQQHAPLLNMS
                     FDL"
     gene            complement(15800..17170)
                     /locus_tag="ACHOUK_002931"
     CDS             complement(15800..17170)
                     /locus_tag="ACHOUK_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HFP9898336.1"
                     /translation="MARSFKVLSPTAILGYGFPEESFRKAMAESPDLIAVDAGSSDPG
                     PHYLGAGKPFTDRAGVKRDLRYMITAGVQNNIPVVIGTAGGSGAAPHLEWCREIIHEI
                     AREEHLSFSMALIPADVDKAIVHQALDNGKITALDFVPPLTHDAIDESTYIVAQMGIE
                     PFQRALKERAQVVLGGRAYDPACFAALPIMQGFDEGLALHCGKILECAAIAATPGSGS
                     DCAMGIIDDNGFTLKTFNPKRKFTETSAAAHTLYEKSDPYFLPGPGGVLNLKGCTFKA
                     VNDGEVYVSGSKHEETPYALKLEGARRVGFRCLTIAGTRDPIMIAGIDKIIDEVKTSV
                     SRNLSLDDDSIRINFHLYGKNGVMGDHEPMQTAGHELGIVLDVVAPTQEIANSVCSLV
                     RSTMLHYGYENRIATAGNLAFPFSPSDIQGGPVYEFSIYHLIEANDALRFDFHIEQVT
                     PEGVQA"
     gene            complement(17174..18415)
                     /locus_tag="ACHOUK_002932"
     CDS             complement(17174..18415)
                     /locus_tag="ACHOUK_002932"
                     /EC_number="4.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050096 - methylaspartate ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate ammonia-lyase"
                     /protein_id="HFP9898337.1"
                     /translation="MKIKQALFTAGYSSFYFDDQQAIKNGAGHDGFFYTGEPVTQGFN
                     AVRQAGECVSVQLILENGAVAVGDCTAVQYSGAGGRDPLFLAEHFIPFLNDHIKPLLV
                     GRDVDAFLPNARFFDKLRIDGNLLHTAVRYGLSQALLDATALATGRLKTEVVCDEWQL
                     PRVAESIPLFGQSGDDRYIAVDKMILKGIDVLPHALINNVEEKLGFKGEKLREYVRWL
                     SDRILSKRTSARYHPTLHIDVYGTIGLIFDMDPLRCAQYIASLEKEAQGLPLYIEGPV
                     DAGNKPDQIRLLTAITKELTRLGSGVKIVADEWCNTYQDIVDFTDAASCHMVQIKTPD
                     LGSIHNIVDAVLYCNSHSMEAYQGGTCNETDVSARTCVHVALAARPMRMLVKPGMGFD
                     EGLDIVFNEMNRTIALLQAKD"
     gene            complement(18415..19860)
                     /locus_tag="ACHOUK_002933"
     CDS             complement(18415..19860)
                     /locus_tag="ACHOUK_002933"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit E"
                     /protein_id="HFP9898338.1"
                     /translation="MELRNKKLTHDEFMTERQQVLKTWETGKDVENFEDGVKYQQTIP
                     EHKRFSLALLKADKEGKTLSQPRAGVALMDEHIELLKTLQEECDLLPSTIDAYTRLNR
                     YEEAAVGIKKSIEAGTSKLNGLPVVNHGVAACRRLTETLQKPLQIRHGTPDARLLAEI
                     SMASGFTSYEGGGISYNIPYAKRVTLEKSIRDWQYCDRLMGMYEEHGIRINREPFGPL
                     TGTLIPPFISHSIAIIEGLLALEQGVKSITVGYGQVGSLTQDVAAIQSLRELAHEYFQ
                     SYGYTDYELSTVFHQWMGGFPEDESKAFAIISWGAAVAGMSGATKVITKSPHEAWGIP
                     TAAANIQGLKASRQMLNMVNEQKFPPCPAVELEIELIKSEVRAVLNKVFELGNGDIAR
                     GTVLAFEAGVLDVPFAPAACNAGKILPVRDNTGAIRVLEAGAVPLPKDILDLHHDYVA
                     ERARCEGRQPTFQMVVDDINAVSHSKLIGRP"
     gene            complement(19879..21267)
                     /gene="glmL"
                     /locus_tag="ACHOUK_002934"
     CDS             complement(19879..21267)
                     /gene="glmL"
                     /locus_tag="ACHOUK_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase accessory protein GlmL"
                     /protein_id="HFP9898339.1"
                     /translation="MQIVSVDIGSTWTKAALFTREGDALTLVNHVLTPTTTHHLAKGF
                     FSSLNQVLNVDNALPLLSSGEVALKYSSSAKGGLAVAAMGLVPSITLETAKVTAHSAG
                     AKIAQYYAYKLNRRDIQALEETQPDILLFTGGTDGGEESYGLNNARVLAESKLDCAII
                     YAGNRDIQDEVQEILGHKDLTIVDNVLPDLDHPNPVAARQAICDIFLKRIVKGKGLDV
                     IVDKTGEEPMPTPWTVFELVKAISNVDHSWKEFMLIDMGGATTDVYSACANTLSPDTV
                     LHGVPEPFVKRTVEGDLGMRVSAVVVGESAKELVNVNFAQQPAQLDAFYHYLRHLVAH
                     PDYLPANAEEKYFDTVLAGLCVGYATERHAGTKKEVCTCVGNVDLQMGRDLTTVRKVV
                     GSGGWLSRASQFDMHHWLKYRELNDDGKRILLPTEFEYYRDSRGLLPLLANVARVDPL
                     AAARTSIQCLTL"
     gene            complement(21267..21716)
                     /gene="glmS"
                     /locus_tag="ACHOUK_002935"
     CDS             complement(21267..21716)
                     /gene="glmS"
                     /locus_tag="ACHOUK_002935"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit S"
                     /protein_id="HFP9898340.1"
                     /translation="MKKATLVIGVIGADCHAVGNKVLDRVFSNHDFRVINLGVMVSQD
                     EYIDAAIETGADAIVVSSIYGHGDIDCLGMRERCIERGLGDILLYVGGNLVVGKHDFA
                     DVETKFKEMGFDRVFAPSHDLEDVCQLMAHDINQRHDVDTRILEEAI"
     gene            complement(22264..22686)
                     /locus_tag="ACHOUK_002936"
     CDS             complement(22264..22686)
                     /locus_tag="ACHOUK_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9898341.1"
                     /translation="MIPSQISFNTLPVNATYASETTVDTQKFSDILYSAGCSLKDMAY
                     LSRCLASIHPTLAKNIYETENLSDQKLLHIDCRSTNEIKINIFFGQQREGLIEINSDT
                     VIFSILSSLIDSAISKFQHQIPVNGSVNRELLYEDYTD"
BASE COUNT         5465 a         5672 c         5944 g         5656 t
ORIGIN      
        1 gtttgtaaca actttgttga atgattgtca aattagatga ttaaaaatta aataaatgtt
       61 gttatcgtga cctggatcac tgttcaggat aaaacccgac aaactatatg taggttaatt
      121 gtaatgattt tgtgaacggc ctatactgcc gccaggtctc cggaacaccc tgcaatcccg
      181 agccacccag cgttgtaacg tgtcgttttc gcatctggaa gcagtgtttt gcatgacgcg
      241 cagttataga aaggacgctg tctgacccgc aagcagaccg gaggaaggaa atcccgacgt
      301 ctccaggtaa cagaaagtta acctctgtgc ccgtagtccc cagggaataa taagaacagc
      361 atgtgggcgt tattcatgat aagaaatgtg aaaaaacaaa gacctgttaa tctggaccta
      421 cagaccatcc ggttccccat cacggcgata gcgtccattc tccatcgcgt ttccggtgtg
      481 atcacctttg ttgcagtggg tatcctgctg tggcttctgg gtaccagcct ctcttcccct
      541 gaaggtttcg agcaagcttc cgcgattatg ggcagcttct tcgtcaaatt tatcatgtgg
      601 ggcatcctta ccgctctggc gtatcacgtc gtcgtaggta ttcgccacat gatgatggat
      661 tttggctatc tggaagaaac attcgaagcg ggtaaacgct ccgccaaaat ctcctttgtt
      721 attactgtcg tgctttcact tctcgcagga gtcctcgtat ggtaagcaac gcctccgcat
      781 taggacgcaa tggcgtacat gatttcatcc tcgttcgcgc taccgctatc gtcctgacgc
      841 tctacatcat ttatatggtc ggttttttcg ccaccagtgg cgagctgaca tatgaagtct
      901 ggatcggttt ctgcgcctct gcgttcacca aagtgttcac cctgctggcg ctgttttcta
      961 tcttgatcca tgcctggatc ggcatgtggc aggtgttgac cgactacgtt aaaccgctgg
     1021 ctttgcgcct gatgctgcaa ctggtgattg tcgttgcact ggtggtttac gtgatttatg
     1081 gattcgttgt ggtgtggggt gtgtgatgaa attgccagtc agagaatttg atgcagttgt
     1141 gattggtgcc ggcggcgcag gtatgcgcgc ggcgctgcaa atttcccaga gcggccagac
     1201 ctgtgcgctg ctctctaaag tcttcccgac ccgttcccat accgtttctg cgcaaggcgg
     1261 cattaccgtt gcgctgggta atacccatga agataactgg gaatggcata tgtacgacac
     1321 cgtgaaaggg tcggactata tcggtgacca ggacgcgatt gaatatatgt gtaaaaccgg
     1381 gccggaagcg attctggaac tcgaacacat gggcctgccg ttctcgcgtc tcgatgatgg
     1441 tcgtatctat caacgtccgt ttggcggtca gtcgaaaaac ttcggcggcg agcaggcggc
     1501 acgcactgcg gcagcggctg accgtaccgg tcacgcactg ttgcacacgc tttatcagca
     1561 gaacctgaaa aaccacacca ccattttctc cgagtggtat gcgctggatc tggtgaaaaa
     1621 ccaggatggc gcggtggtgg gttgtaccgc actgtgcatc gaaaccggtg aagtggttta
     1681 ttttaaagcc cgcgctaccg tgctggcgac tggcggagca gggcgtattt atcagtctac
     1741 cactaacgcc cacattaaca ccggcgacgg cgtcggcatg gctatccgcg caggcgtacc
     1801 ggtgcaggat atggaaatgt ggcaattcca cccgaccggc attgccggtg cgggcgtact
     1861 ggtcaccgaa ggttgccgtg gtgaaggcgg ttatctgctg aacaaacatg gcgaacgctt
     1921 tatggagcgt tatgcgccga acgccaaaga cctggcgggc cgtgatgtgg tggcgcgttc
     1981 aatcatgatt gaaatccgtg aaggtcgcgg ctgtgatggt ccgtgggggc cacacgcgaa
     2041 actgaaactc gatcacctgg gtaaagaagt tctcgaatcc cgtctgccgg gtatcctgga
     2101 gctttcccgt accttcgctc acgttgatcc ggtgaaagag ccgattccgg ttatcccaac
     2161 ctgtcactac atgatgggcg gtattccgac caaagttacc ggtcaggcgc tgactgtgaa
     2221 tgagaaaggc gaagatgtgg ttgttccggg gctgttcgcg gtaggtgaaa ttgcctgcgt
     2281 atcggtacac ggtgctaacc gtctgggcgg caactcgctg ctggacctgg tggtctttgg
     2341 tcgcgcagcg ggtctgcatc tgcaagagtc tatcgccgag cagggcgcat tgcgcgatgc
     2401 cagcgagtct gatgttgaag catctttgga tcgtctgaac cgctggaaca ataatcgtaa
     2461 cggtgaagat ccggtggcga ttcgtaaagc gctgcaagaa tgtatgcagc ataacttctc
     2521 ggtcttccgt gaaggtgatg cgatggctaa agggcttgag cagttgaaag tgatccgcga
     2581 gcgtctgaaa aatgcccgtc tggatgacac ttccagcgag ttcaacaccc agcgcgttga
     2641 gtgcctggaa ctggataacc tgatggaaac ggcgtatgca acggctgttt ctgccaactt
     2701 ccgtaccgaa agccgtggcg cacatagccg cttcgacttc ccggatcgtg atgatgaaaa
     2761 ctggctgtgc cactccctgt atctgccaga gtcggaatcc atgacgcgcc gaagcgtcaa
     2821 catggaaccg aaactgcgcc cggcattccc gccgaagatt cgtacttact aatgcggaga
     2881 caggaaaatg agactcgagt tttcaattta tcgctataac ccggatgttg atgatgctcc
     2941 gcgtatgcag gattacaccc tggaagcgga agaaggtcgc gacatgatgc tgctggatgc
     3001 gcttatccag ctgaaagaga aagatcccag cctgtcgttc cgccgctcct gccgtgaagg
     3061 tgtgtgcggt tccgacggtc tgaacatgaa cggcaagaat ggtctggcct gtattacccc
     3121 gatttcggca ctcaaccagc cgggcaagaa gattgtgatt cgcccgctgc caggtttacc
     3181 ggtgatccgc gatttggtgg tagacatggg acaattctat gcgcaatatg agaaaattaa
     3241 gccttacctg ttgaataatg gacaaaatcc gccagctcgc gagcatttac agatgccaga
     3301 gcagcgcgaa aaactcgacg ggctgtatga atgtattctc tgcgcatgtt gttcaacctc
     3361 ttgtccgtct ttctggtgga atcccgataa gtttatcggc ccggcaggct tgttagcggc
     3421 atatcgtttc ctgatcgata gccgtgatac cgagactgac agccgcctcg acggtttgag
     3481 cgatgcattc agtgtattcc gctgtcatag catcatgaac tgcgtcagtg tatgtccgaa
     3541 ggggctgaac ccgacgcgcg ccatcggcca tatcaagtcg atgttgttgc aacgtaatgc
     3601 gtaaaccgta ggcctgataa gacgcgcaag cgtcgcatca ggcaaccagt gccggatgcg
     3661 gcgtgaacgc cttatccggc ctacaagtca ttacccgtag gcctgataag cgcagcgcat
     3721 caggcgtaac aaagaaatgc aggaaatctt taaaaactgc ccctgacact aagacagttt
     3781 ttaaaggttc cttcgcgagc cactacgtag acaagagctc gcaagtgaac cccggcacgc
     3841 acatcactgt gcgtggtagt atccacggcg aagtaagcat aaaaaagatg cttaagggat
     3901 cacgatgcag aacagcgctt tgaaagcctg gttggactct tcttacctct ctggcgcaaa
     3961 ccagagctgg atagaacagc tctatgaaga cttcttaacc gatcctgact cggttgacgc
     4021 taactggcgt tcgacgttcc agcagttacc tggtacggga gtcaaaccgg atcaattcca
     4081 ctctcaaacg cgtgaatatt tccgccgcct ggcgaaagac gcttcacgtt actcttcaac
     4141 gatctccgac cctgacacca atgtgaagca ggtaaaagtc ctgcagctca ttaacgcata
     4201 ccgcttccgt ggtcaccagc atgcgaatct cgatccgctg ggactgtggc agcaagataa
     4261 agtggccgat ctggatccgt ctttccacga tctgaccgaa gcagacttcc aggagacctt
     4321 caacgtcggt tcatttgcca gcggcaaaga aaccatgaaa ctcggcgagc tgctggaagc
     4381 cctcaagcaa acctactgcg gcccgattgg tgccgagtat atgcacatta ccagcaccga
     4441 agaaaaacgc tggatccaac agcgtatcga gtctggtcgc gcgactttca atagcgaaga
     4501 gaaaaaacgc ttcctaagcg aactgaccgc cgctgaaggc cttgaacgtt acctcggtgc
     4561 aaaattccct ggcgcaaaac gcttctcgct ggaaggcggt gacgcgttaa tcccgatgct
     4621 taaagagatg attcgccacg ctggcaacag cggcacccgc gaagtggttc tcgggatggc
     4681 gcaccgtggt cgtctgaacg tgctggtgaa cgtgctgggt aaaaaaccgc aagacttgtt
     4741 cgacgagttt gccggtaaac ataaagaaca cctcggcacg ggtgacgtga aataccacat
     4801 gggcttctcg tctgacttcc agaccgatgg cggcctggta catctggcgc tggcgtttaa
     4861 cccgtctcac cttgagattg tcagcccggt cgttatcggt tctgttcgtg cccgtctgga
     4921 cagacttgat gagccgagca gcaacaaagt gctgccaatc accattcacg gtgacgccgc
     4981 agtgaccggg cagggcgtgg ttcaggaaac tctgaacatg tcgaaagcgc gtggttatga
     5041 agttggcggt acggtacgta tcgttatcaa caaccaggtt ggcttcacca cctctaaccc
     5101 gctggatgcc cgttcaacgc cgtactgtac tgatatcggt aagatggttc aggcaccgat
     5161 ttttcacgtt aacgcggatg atccggaagc cgttgccttt gtgacccgtc tggcgctcga
     5221 tttccgtaac acctttaaac gtgatgtctt catcgacctg gtgtgctacc gccgtcacgg
     5281 ccacaacgaa gccgacgagc cgagcgcaac ccagccgctg atgtatcaga aaatcaaaaa
     5341 acatccgacg ccgcgcaaaa tctatgctga caagctggag caggaaaaag tggcgacgct
     5401 ggaagatgcc accgagatgg ttaacctgta ccgcgatgcg ctggatgctg gcgattgcgt
     5461 agtggcagag tggcgtccga tgaacatgca ctctttcacc tggtcgccgt acctcaacca
     5521 cgaatgggac gaagagtacc cgaataaagt tgagatgaag cgcctgcagg agctggcgaa
     5581 acgcatcagc acggtgccgg aagcggttga aatgcagtct cgcgttgcca aaatttatgg
     5641 cgatcgccag gcgatggctg ccggtgagaa actgttcgac tggggcggcg cggaaaacct
     5701 cgcttacgcc acgctggttg acgaaggcat tccggttcgc ctgtcgggtg aagactccgg
     5761 tcgcggtacc ttcttccacc gccacgcggt gatccacaac cagtctaacg gttccactta
     5821 cacgccgctg caacacatcc ataacggcca gggcgcgttc cgtgtctggg actccgtact
     5881 ttctgaagaa gcagtgctgg cgtttgaata cggttatgcc accgcagaac cacgcactct
     5941 gactatctgg gaagctcagt tcggtgactt cgccaacggt gcgcaggtgg ttatcgacca
     6001 gttcatctcc tctggcgaac agaaatgggg ccggatgtgt ggtctggtga tgttgctgcc
     6061 gcacggttac gaagggcagg ggccggagca ctcctccgcg cgtctggaac gttatctgca
     6121 actttgtgct gagcaaaaca tgcaggtgtg cgtaccgtct accccggcac aggtttacca
     6181 catgctgcgt cgtcaggcgc tgcgcgggat gcgtcgtccg ctggtcgtga tgtcgccgaa
     6241 atccctgctg cgtcatccgc tggcggtttc cagcctcgaa gaactggcga acggcacctt
     6301 cctgccagcc atcggtgaaa tcgacgagct tgatccgaag ggcgtgaagc gcgtagtgat
     6361 gtgttctggt aaggtttatt acgacctgct ggaacagcgt cgtaagaaca atcaacacga
     6421 tgtcgccatt gtgcgtatcg agcaactcta cccgttcccg cataaagcga tgcaggaagt
     6481 gttgcagcag tttgctcacg tcaaggattt tgtctggtgc caggaagagc cgctcaacca
     6541 gggcgcatgg tactgcagcc agcatcattt ccgtgaagtg attccgtttg gggcttctct
     6601 gcgttatgca ggccgcccgg cctccgcctc tccggcggta gggtatatgt ccgttcacca
     6661 gaaacagcaa caagatctgg ttaatgacgc gctgaacgtc gaataaataa aggatacaca
     6721 atgagtagcg tagatattct ggtccctgac ctgcctgaat ccgtagccga tgccaccgtc
     6781 gcaacctggc ataaaaaacc cggtgacgca gtcgtacgtg atgaagtgct ggtagaaatc
     6841 gaaactgaca aagtggtact ggaagtaccg gcatcagcag acggcattct ggatgcggtt
     6901 ctggaagatg aaggtacaac ggtaacgtct cgtcagatcc ttggtcgcct gcgtgaaggc
     6961 aacagcgccg gtaaagaaac cagcgccaaa tctgaagaga aagcgtccac tccggcgcaa
     7021 cgccagcagg cgtctctgga agagcaaaac aacgatgcgt taagcccggc gatccgtcgc
     7081 ctgctggctg aacataacct cgacgccagc gccattaaag gcaccggtgt gggtggtcgt
     7141 ctgacccgtg aagatgtgga aaaacatctg gcgaaagctc cggcgaaaga gtctgcaccg
     7201 gcagcggctg ctccggcggc gcaaccggct ctggctgcac gtagcgaaaa acgtgtcccg
     7261 atgactcgcc tgcgtaagcg tgtggcagag cgtctgctgg aagcgaaaaa ctccaccgcc
     7321 atgctgacca cgttcaacga agtcaacatg aagccgatta tggatctgcg taagcagtac
     7381 ggtgaagcgt ttgaaaaacg ccacggcatc cgtctgggct ttatgtcctt ctacgtgaaa
     7441 gcggtggttg aagccctgaa acgttacccg gaagtgaatg cttctatcga cggcgatgac
     7501 gtggtttacc acaactattt cgacgtcagc atggcggttt ctacgccgcg cggcctggtg
     7561 acgccggtac tgcgtgatgt cgataccctc ggcatggcag acatcgagaa gaaaatcaaa
     7621 gagctggcag ttaaaggccg tgacggcaag ctgacggttg aagatctgac cggtggtaac
     7681 ttcaccatca ccaacggtgg tgtgttcggt tccctgatgt ctacgccgat catcaacccg
     7741 ccgcagagcg caattctggg tatgcacgct atcaaagatc gtccgatggc tgtgaatggt
     7801 caggttgaga tcctgccgat gatgtacctg gcgctgtcct acgatcaccg tctgatcgat
     7861 ggtcgcgaat ccgtgggctt cctggtaacg atcaaagagt tgctggaaga tccgacgcgt
     7921 ctgctgctgg acgtgtagta gtttaagttt cacctgcact gtagaccgac gtggataggg
     7981 tgtcggtcta cggtttaaaa gataacgatt actgaaggat ggacagaaca catgaactta
     8041 catgaatatc aggcaaaaca actgtttgcc cgctatggct taccagcacc ggtgggttat
     8101 gcctgtacta ctccgcgcga agcagaagaa gccgcttcaa aaatcggtgc cggtccgtgg
     8161 gtagtgaaat gtcaggttca cgctggtggc cgcggtaaag cgggcggtgt gaaagttgta
     8221 aacagcaaag aagacatccg tgcttttgca gaaaactggc tgggcaagcg tctggtaacg
     8281 tatcaaacag atgccaatgg ccaaccggtt aaccagattc tggttgaagc agcgaccgat
     8341 atcgctaaag agctgtatct cggtgccgtt gtagaccgta gttcacgtcg cgtggtcttt
     8401 atggcctcca ccgaaggcgg cgtggaaatc gaaaaagtgg cggaagaaac tccgcacctg
     8461 atccacaaag ttgcgcttga tccgctgact ggcccgatgc cgtatcaggg acgcgagctg
     8521 gcgttcaaac tgggtctgga aggtaaactg gttcagcagt tcaccaaaat cttcatgggc
     8581 ctggcgacca ttttcctgga gcgcgacctg gcgctgatcg aaatcaaccc gctggtcatc
     8641 accaaacagg gcgatctgat ttgcctcgac ggcaaactgg gcgctgatgg caacgccctg
     8701 ttccgccagc ctgatctgcg cgaaatgcgt gaccagtcgc aggaagatcc gcgtgaagca
     8761 caggctgcac agtgggaact gaactacgtt gcgctggacg gtaacatcgg ttgtatggtt
     8821 aacggcgcag gcctggcgat gggtacgatg gacatcgtta aactgcacgg cggcgaaccg
     8881 gctaacttcc tggacgttgg cggcggcgca accaaagaac gtgtaaccga agcgttcaaa
     8941 atcatcctct ctgacgacaa agtgaaagcc gttctggtta acatcttcgg cggtatcgtt
     9001 cgttgcgacc tgatcgctga cggtatcatc ggtgcggtag cggaagtggg tgttaacgta
     9061 ccggtcgtgg tacgtctgga aggtaacaac gccgaactcg gcgcgaagaa actggctgac
     9121 agcggcctga atattattgc agcaaaaggt ctgacggatg cagctcagca ggttgttgcc
     9181 gcagtggagg ggaaataatg tccattttaa ttgataaaaa caccaaggtt atctgccagg
     9241 gctttaccgg tagccagggg actttccact cagaacaggc cattgcatac ggcactaaaa
     9301 tggttggcgg cgtaacccca ggtaaaggcg gtactaccca cctcggcctg ccggtgttca
     9361 acaccgtgcg tgaagctgtt gctgctactg gcgctaccgc ttctgttatc tacgtaccag
     9421 caccgttctg caaagactcc attctggaag ccatcgacgc aggcatcaaa ctgattatca
     9481 ccatcactga aggcatcccg acgctggata tgctgaccgt gaaagtgaag ctggatgaag
     9541 caggcgttcg tatgatcggc ccgaactgcc caggcgttat cactccgggt gaatgcaaaa
     9601 tcggtattca gcctggtcac attcacaaac cgggtaaagt gggtatcgtt tcccgttccg
     9661 gtacactgac ctatgaagcg gttaaacaga ccacggatta cggtttcggt cagtcgacct
     9721 gtgtcggtat cggcggtgac ccgatccctg gctctaactt tatcgacatt ctcgaaatgt
     9781 tcgaaaaaga tccgcagacc gaagcgatcg tgatgatcgg tgagatcggc ggtagcgctg
     9841 aagaagaagc agctgcgtac atcaaagagc acgttaccaa gccagttgtg ggttacatcg
     9901 ctggtgtgac tgcgccgaaa ggcaaacgta tgggccacgc gggtgccatc attgccggtg
     9961 ggaaagggac tgcggatgag aaattcgctg ctctggaagc cgcaggcgtg aaaaccgttc
    10021 gcagcctggc ggatatcggt gaagcactga aaactgttct gaaataaata tctgtaataa
    10081 gaaatagccc tcgccgcttc cctctacagg aatggcgaag ggctgtcggt ttcgacatgg
    10141 ttggccatcg tatgatggcc ttttttatgc cttaaacggt taaccacggt cgaaatctga
    10201 ctgcaggctt gactgcatga ccacagccac tgtggattgc cgcaaccgac cgcaagccac
    10261 gaataagaac gacggcgaat ttcgaatcgt tacctgatgc actggcttcc cagcgtttga
    10321 ccgtagcgtt aagtaaatca acaaaagcga tgttcaccgc ttttcgtcaa gtcgtgccat
    10381 ttgtcagcca ggatcgggtc tttaccccgg atattgcagc atcgcaaaaa ttcttagagg
    10441 gatatgctat aaatcaaaaa taaaatcttt gttgattgtt tatcataata tccacagata
    10501 tgataaatga tatcaattta ataattttca cttaatgtga ttccggataa ttcgaaaggc
    10561 gctgtgcttt tcggattatt attatctgat taagatattt gtcacgccag ccttactggt
    10621 ttataaatgt caatcatgat tcttataaat tatttattgc tgctttggtt accattactt
    10681 cgtaataagt aatgtatttt tgatggttta aactattttt aagggacggc attatgcgag
    10741 ggaaaatacc taaaacggaa ctgctggtga cttttgaggt cgtcgcacgt catgaaagtt
    10801 ataccagagc ggcagaggag ttagcgttaa cccaaagtgc agtattcaga caagtaagtg
    10861 cgctggagga atttttacgt actccgttat tttcacactc taaaaaacgc atttttctta
    10921 atgatacagg gaagtattac ctggggatag taaaagaaac attaaataag ctggaacgcg
    10981 ataccaatac cattatgacc tggcagccta cggttcaggt tattgaactt gccgttaatc
    11041 ctacttttag tacccactgg ttaatcccaa atttacatga attcacaaag ttacatcccg
    11101 atattattgt taatattcat tctttagcta ataacggcga ttttcttaat cgggagtacg
    11161 atgctgtcat tatgcgcgaa aatttttgcg cgccctgggc ggaagtagaa tatctctttg
    11221 aagaggagat tttgccggta tgcagcggca gtttgcttgc aatgtccgat caaaaactgt
    11281 cagtggcaga actgcttact gagttaccac ttctgcatca aagcacccgt attactggct
    11341 gggaagagtg gtttgcttta tctggcgtta gcagtccgct ggtcaacaac ggaccacgtt
    11401 tcgatttgct ttcaatgctg attgccgccg tgcgctctaa tctcggcgtg gcgctgttac
    11461 cacgtttcgc tattcaacat gatttggata gtggcgatat ggtgattccc tgtgatgtgc
    11521 ctattcgtac cggtaatcgt tttattatga cgtggcagga agagaagtct gactcaccgc
    11581 acttgcagca atttcgtgaa tggttgctgg cgaaatcggt cgtaccgcag gagatgtaaa
    11641 acctgcgcct ggcgggatgc caggcgcagt tattaccagt cgatacccgg ctgggcctga
    11701 atgccgttat caaaagcatg tttgacgggg cggatttcgc tgaccgtatc tgccattttt
    11761 aatatctgac tgtggcaacc gcgtccggtg acaatgacac tttgttgtgc cgggcggttt
    11821 tgcagagaag cgatcacttc ttcggtatcc agataatggt aagccagcat ataggtcagc
    11881 tcatcaagca ccactaagtc gtaacgcttg tcagccagca tccgtttgct ttccgaccag
    11941 acctctttcg ccgcgtcgat atccgcttgc cggttctgtg tctcccaggt aaaaccggtt
    12001 cccataatat gaaactcaac ccccagcggc tggagaaggt tgtattcgcc gttgtcccat
    12061 tgccccttga tgtactgcgc aacccccacc gttttgccat gacccaccgc gcgcgtcaca
    12121 gtaccaaacg cggcagtgga tttccctttg ccgttgccgg tgaaaacaat caagatccct
    12181 tttttctcta tcgccgccgc gacgcgcgta tcgacctgtt ccttcaattt ctgttggcgc
    12241 tgcacatgcc gttcagttga aattcgcgct tccattcatt ttccttaatg tcccgcaggg
    12301 caactggcgc agcggtgttg ctgctcaaac tgactaaaga aattattgcc tttatcatct
    12361 accaggatga atgcgggcag attttccact tccatcatcc acaccgcttc catacccagc
    12421 tccgggtatt ccaggcaacg cagacttttg acatattctt gcgccagtaa cgcagctgca
    12481 ccgccaatac ttcccagatt gaagccgcca tgtttgtgac aggcatcggt gacctgctgg
    12541 ctgcggttac ctttcgataa catcactaga ctgccgccag ccgcctggaa ggtatcgata
    12601 taaccgtcca ttcgaccgcc cgtggttgga cccagtgaac cgcaggccat gttctccggt
    12661 gttttcgccg ggccagcgta atagacaata tgatgcttca ggtactccgg catcggttcg
    12721 ccgctatcca gccgcgcttt tattttggcg tgggcgatat cgcgcgccac tacgatggga
    12781 ccacttaacg acacgcgggt gcctactggt aatctggcga gatcctgcat cacgtcacgc
    12841 aacggacggt ttagatccag ttgtacatgt tgcgcgtgat tttcttcccg cagtgaggct
    12901 ggaatatatt gccccggatt gtgttcgagt ttttccagcc agataccgtg tttgttaatt
    12961 tttgctttga tattgcgatc ggcagaacag gagagcgcca tagctatcgg gcaagagcca
    13021 ccgtgacgcg gcagacgaat gacgcgaata tcatgagcga aatatttacc cccaaattgc
    13081 gcaccaatac caaattgctg actggcctcg agcaacactt tttccagctc aatatcacgg
    13141 aatgcttgtc cctgctcgtt tccgctggtg ggcaaattgt cgtaatattt cgttgaggcc
    13201 agcttggcaa tttttaaggt ttgatcggct gaaagccctc cgacgacaaa ggcgatgtgg
    13261 taaggcgggc aggcagcggt gcccagcgat ttcatttttt cgattaaaaa tgcggtcagt
    13321 ttttctggtt gcaataagga tttggtttcc tgataaagcg cggctttatt ggcggagccg
    13381 ccccctttat taacacagag aaaatggtac tcatcaccag cgacagcgct gatatcaatc
    13441 tgcgccggga gattggtttg cgtattgact tcggtataca tatccagcgg cgcattttgt
    13501 gaaaagcgca gattattttc ctggaaagta ctgtaaatgc ctttacttaa ggcttcggcg
    13561 tcgttacctc cggtccatat ctgttgccct ttactggcaa caatagttgc ggtgccggta
    13621 tcctgacagt tgggtaatac cccttttgct gagacttcag cattacgtaa taactgcaac
    13681 gcaacgtatt tatcattact gctggcctgc tgatcgttaa gaatgctggc gacctgttgc
    13741 aaatgagcgg aacgtaagaa aaaagaggct tcataaaatg cctgccgggc aagcagcgtt
    13801 aatgcttcag gagcgatttt tatcacttct tcgccgtcta attccgttac tgtaatatgt
    13861 tgatcgctaa ttaatgtata ttctgtaccg tctttatttt gtagaaacgg ttcttgccag
    13921 ataaatggtt tggacatatt tcacttttca ccttaaacgt tagtgttact taattgccgt
    13981 ctaacttcat gatccggctt ataaaaatgc gtagtcattg gcttctgatt gcttaagatc
    14041 cccctcaggg cagagcaacg ttcgattcat tgggttttaa tgctttaccc cgacagccct
    14101 cgccgaaatg ttcgccttcc caccgactca ccatcgcagc gcccattgcg ttgctcatga
    14161 cgtttgtcgc ggaacgcccc atatcaagga acggatcaac gcccatcagc aagatcagcc
    14221 ccgcttccgg aatattgaac tggttgagcg tggcggcgat aaccaccatt gaggcgcgtg
    14281 gtacgccagc cattcctttc gaggtcaaca tcaggatcaa cagcatggtg atttgctcac
    14341 cgatggataa atggatattg caggcctggg cgatgaaaac tgtggcgaag gagcagtagg
    14401 ccattgatcc aacgagatta aatgagtagc caatgggtaa gacaaagctg gcaattttgg
    14461 gggaaacgcc aaattgctcc agtttttcaa gcgttcccgg aaaagccgct tcagaactgg
    14521 atgtggtaaa tgccagcagg gcgggttccg aaagggcgcg ggtcaggcgt ctgatgcagg
    14581 ggccgacata aacgatggcc agaccgataa gcagtaccca aagtaataac atggtgaaat
    14641 aaaattcacc cataaagatc ccggcgctca ccataactgc cagtcctcgt tcagcaatca
    14701 atgctgaaat agcagcgaat acggtcaggg gagcgaagag catgacgtag ccagtgagct
    14761 ttaacatggc atgtgccagc gaatctaagg cgtgaacgat ggcgctgcct ttctcgccaa
    14821 tcgccgtcag gctacagccg aggaaaattg agaacaccac gatttgcaaa atttcattgt
    14881 gcgccatggc atcgacaatg ctggtgggga aagcatgcga aataaatact tttagcgtaa
    14941 agggttctgc cgcgaccact ccggtggttt cggcaccgtg tgcaacaaaa ttaattcctg
    15001 tacctggcat gaagaaattt accgttatca agcctaaggc aattgacagc aatgaggcgc
    15061 aaataaataa aaagagtgtt ttagaaaaaa tacgaccaag ggctttggca tcgcccattt
    15121 tagcaatacc taccaccagg gtagagacca ctaacggagc gataatcatt tttatcagtc
    15181 gtaagaaaat cgtcgtgaat atcgatattt cttgcgcata gagttttgcg gtttccggtg
    15241 aagcagtgtt attgagcact acgccgataa tcattccaag taccagcgcc aaaagaatca
    15301 tcgtggttaa acttattttc ttcatttttt cctccatcag gtgcacggcg ataaatcaga
    15361 atcaggagaa gtagatgcct gaatagagat tcaggcatct acctgctatt acaaccttac
    15421 aaatagctga agtgttgaac tgcaataagt gacgggatgt cagttggcta aaagtattcg
    15481 gatttttaca aatcgaaact catatttaat aaaggtgcat gttgctgtgc accatagaca
    15541 tcattgtcac ctacattccc ggagataata tctcgtggca tgacgatttt tactgcgtta
    15601 gaaggatcaa accagacaat acgatgaata aagtcaggtt caacatgata taagcgtgca
    15661 atcagttctg gggttaattg ccctgatgat ttaactcgct catagttttc tttcgtttta
    15721 aataaaatat ctaaaaccag ttcgtaaggt ccggcatttt tcgaacgaat gacttgcgcc
    15781 agtgagacaa ttgactgttt catgcctgaa ctccttctgg cgtcacctgt tcaatatgga
    15841 aatcaaaacg cagagcgtcg ttggcttcaa tcaggtgata tatggaaaat tcgtataccg
    15901 gcccgccctg gatatcagaa ggggagaacg ggaacgccag attacctgcg gtagcgatgc
    15961 ggttttcata gccgtagtgc agcatggtag agcgcaccag cgagcaaacg ctgttggcaa
    16021 tttcctgggt cggtgcaact acatcaagga caatccccag ctcatgcccg gcagtttgca
    16081 tcggttcatg gtctcccatc accccgtttt tgccgtacag gtggaaattg atgcgaatgc
    16141 tgtcatcgtc gagcgacagg ttacgcgaaa cgctggtttt gacttcatcg atgattttat
    16201 cgatcccggc gatcataatc gggtcgcgcg ttccggcgat ggtcagacag cggaagccca
    16261 cccgtcgtgc accttccagt ttcagggcat acggcgtttc ttcatgctta gaaccgctga
    16321 cgtagacttc gccgtcattt actgctttaa atgtgcagcc tttcaggttc aacacgccgc
    16381 caggtcctgg caggaagtag ggatcggatt tctcatacag tgtgtgtgca gctgctgacg
    16441 tttcggtaaa tttacgcttc ggattaaatg ttttcagcgt aaagccgttg tcatcaatga
    16501 tgcccatcgc acagtcagag cctgagccgg gcgttgcggc gattgccgcg cattcaagga
    16561 ttttcccaca atgcagcgcc agaccttcat caaagccttg catgatcggg agcgcagcaa
    16621 agcaggccgg gtcgtaagcg cgcccgccca gcaccacttg cgcgcgttct ttcagcgccc
    16681 gctggaaggg ttcgatgccc atttgcgcga cgatatacgt actttcgtca atcgcgtcgt
    16741 gggttaacgg cgggacaaaa tccagcgccg tgattttgcc gttatccagc gcctggtgga
    16801 cgatggcttt gtcaacgtct gccgggatca acgccatcga gaacgagagg tgctcttccc
    16861 gggcaatctc atgaattatt tctcggcacc actccaggtg tggtgcggca ccagaaccgc
    16921 cagccgttcc gatcaccacc ggtatattat tttgcacgcc tgcggtgatc atatagcgca
    16981 gatcgcgctt gacccctgcg cgatcggtaa acggtttgcc tgccccgagg tagtgggggc
    17041 ctggatcgga agagcctgca tcaacggcga tcagatcggg cgattctgcc atcgctttgc
    17101 ggaagctctc ttccgggaat ccatacccga ggattgccgt tggcgataag actttaaaag
    17161 aacgagccat ttgttaatcc ttagcctgca acagcgcgat agtacgattc atttcgttga
    17221 agacgatatc gaggccttcg tcaaagccca tccctggttt taccagcata cgcatggggc
    17281 gagcggcaag ggcgacgtgg acacaggtgc gggcactgac atcagtttca ttgcaggtgc
    17341 cgccctggta cgcttccatg ctgtggctgt tgcagtaaag aaccgcatcg acgatgttgt
    17401 gaatactgcc cagatccggg gttttgattt gcaccatgtg acaactggca gcatcagtga
    17461 aatcaacaat atcctggtag gtgttacacc attcatcggc cacaattttc acgccggaac
    17521 cgaggcgcgt cagctctttg gtaatcgcgg tcagcaggcg aatttgatcg ggcttgttac
    17581 cggcatcgac cggcccttcg atgtagagcg gcaggccttg cgcttctttt tccaggctgg
    17641 cgatgtattg cgcacagcga agcggatcca tatcgaagat cagaccgata gtgccgtata
    17701 catcgatgtg cagggtaggg tggtagcgtg cgctggtgcg cttgcttaga atgcgatccg
    17761 acaaccagcg gacatattcg cgcagttttt cacctttaaa gcccagcttc tcttcgacgt
    17821 tattaatcag cgcatggggc agcacgtcga tgcctttaag gatcatctta tcgacggcga
    17881 tatatcgatc gtcgccgctc tgaccaaata atggaatgga ttccgccacg cgtggcaact
    17941 gccattcatc acagaccact tcagttttca gacggccggt tgccagcgcg gtagcatcaa
    18001 gcagcgcctg tgataatccg tagcgcacgg cggtatgcag caagttgccg tcaatacgca
    18061 atttgtcgaa gaaacgggca ttcggcagga aagcatccac atcgcggcct accagtaatg
    18121 gcttgatatg gtcgttgagg aacggaataa agtgctctgc gaggaacagt ggatcgcgac
    18181 cgcctgcccc ggaatactgt acggcagtac agtcacctac ggcgaccgcg ccgttttcca
    18241 gaatcaactg taccgatacg cactccccgg cctgacgtac ggcgttaaac ccctgtgtta
    18301 ctggctcccc ggtataaaaa aagccgtcat gacccgctcc gttttttatc gcctgctggt
    18361 catcgaaata gaatgaggag tagccagcgg tgaacagagc ctgttttatt ttcattatgg
    18421 tcttcctatt aatttactgt gggatacagc attgatgtca tctacaacca tctggaatgt
    18481 cggttggcgt ccttcacagc gtgcgcgttc cgcaacgtaa tcgtggtgga ggtcgagaat
    18541 atcttttggc aatggaacgg ctccggcttc cagtacgcgt atcgcgccgg tgttgtcacg
    18601 gaccggaaga attttgcccg cgttgcaggc ggctggcgca aacggcacat ccagtacgcc
    18661 cgcttcaaat gccagcaccg ttccgcgcgc aatatcacca ttacccagtt cgaacacttt
    18721 attcagtacc gcgcgaacct cgctcttaat cagttcaatt tccagttcaa ccgcagggca
    18781 cggcgggaat ttctgctcat tgaccatgtt gagcatttgg cgggaggctt tcagcccctg
    18841 aatgttggca gcggcagtcg ggatacccca ggcttcgtgc ggacttttgg tgatgacttt
    18901 ggtggcacca gacatccccg cgacggctgc gccccaggag ataatagcga acgctttcga
    18961 ttcgtcttcc ggaaagccgc ccatccactg gtgaaacacg gtactcaatt catagtcggt
    19021 atagccatag ctctgaaaat attcgtgtgc aagttcacgc agagactgga tcgccgcaac
    19081 atcctgcgtc aggctgccga cctggccata accaacggtg atagatttca caccctgttc
    19141 cagcgccagc aagccttcga ttatcgcgat agagtgcgag ataaacggcg ggatcagcgt
    19201 acccgttagt gggccaaacg gttcgcggtt gatgcggatc ccgtgttctt catacatccc
    19261 cattaaacgg tcgcagtact gccagtcgcg gatcgatttt tccagcgtta cgcgtttggc
    19321 atacggaatg ttgtaggaaa tgccaccgcc ttcataactg gtaaagccgc tggccattga
    19381 gatttcagcc agcagacgcg catctggcgt gccgtggcgg atctgtagcg gtttttgcag
    19441 ggtttcggtg aggcgacggc aagctgcgac accatggtta actaccggta aaccgttcag
    19501 tttagaggtt ccggcttcga tggatttttt aatccctaca gccgcttctt cgtaccgatt
    19561 cagacgggta tatgcatcga tggtgcttgg cagcagatcg cactcttctt gtagtgtttt
    19621 cagcagttcg atgtgttcgt ccatcagcgc cacacctgcg cgcggttggc taagggtttt
    19681 accttccttg tctgctttca gcagcgcaag agagaaacgt ttatgctcag ggatggtttg
    19741 ttggtattta acgccatctt caaagttttc aacatccttg ccggtttccc aggttttcag
    19801 tacctgttgc cgctcggtca tgaattcgtc atgggttaat ttcttatttc gaagttccat
    19861 tgattgcagc cttaattgtt atagggttaa gcattgaata ctggtgcgcg cggcggccag
    19921 cggatcgact ctggcgacgt ttgccagcag tggtaatagc ccccgggaat cgcggtaata
    19981 ttcaaattcg gtcggtaaga gaatgcgttt accgtcgtcg tttaactcgc ggtatttcaa
    20041 ccagtgatgc atatcgaact gactggcacg cgagagccat ccgccggatc cgacgacttt
    20101 acgcacggtt gtcaggtcac gtcccatctg taaatcgaca ttgccgacgc aggtgcagac
    20161 ctcttttttg gtgcccgcat gtctttcggt ggcataaccc acacacagtc cggccaggac
    20221 ggtatcgaaa tatttctctt ctgcatttgc gggtaagtag tcgggatgag cgactagatg
    20281 acggaggtaa tgataaaaag cgtccagctg tgcgggttgc tgagcaaaat tcacattgac
    20341 taactctttc gcactttcac caaccaccac tgccgagacg cgcattccca gatcgccttc
    20401 caccgtccgt tttacgaagg gttccggtac gccgtgtaaa acggtgtcgg gcgagagcgt
    20461 gttggcacag gcggaataaa catcggtggt tgcgccgccc atgtcgatca gcatgaactc
    20521 tttccagctg tgatcgacgt tactgattgc cttgaccagt tcaaagaccg tccatggtgt
    20581 cggcattggt tcttcgcctg ttttatcaac aatgacatcc agccctttcc ctttaacgat
    20641 gcgtttaagg aatatgtcgc aaatagcctg gcgtgcagct acagggttcg gatggtcaag
    20701 atcgggcaga acgttgtcga caatggtcag gtctttatgc ccgaggatct cctgcacctc
    20761 gtcctgaata tcccggtttc cggcataaat aatggcgcag tcgagtttgg attctgccag
    20821 cacacgggcg ttattcagtc cgtagctttc ttctccgccg tcggtgccgc cggtgaacaa
    20881 gagaatatcg ggctgcgttt cttccagtgc ctggatatca cggcggttca gcttgtaggc
    20941 gtaatattgc gcaattttag cacccgcaga gtgagcggtt actttcgcgg tttccagggt
    21001 gatagaaggc accaatccca ttgcggcaac cgccagccca cctttggcag aggaggaata
    21061 cttcaatgca acctcaccgc tgcttaacag ggggagggca ttatctacgt ttaacacctg
    21121 gttcaggctg gagaaaaacc ccttcgccag atgatgggtg gtggttgggg tcagtacgtg
    21181 gttaaccaac gttaacgcgt ccccctcccg ggtaaagagg gctgctttgg tccacgtcga
    21241 tccaatatcg acagagacga tttgcatcag atagcctctt caagaatacg agtatcaacg
    21301 tcatgacgtt ggttgatatc gtgggccatc agctggcaaa catcttctaa gtcatgactt
    21361 ggggcaaaga cgcgatcaaa tcccatctct ttgaatttgg tttccacgtc agcgaagtca
    21421 tgttttccga ccaccaggtt accgcccaca tagagcagga tgtcccccag tccacgctcg
    21481 atgcagcgtt cgcgcatgcc cagacagtcg atatcgccat gcccatagat agaggagacg
    21541 acaatcgcgt cagcgccagt ttcaatggca gcatcaatat attcgtcctg gctgaccatt
    21601 acgcccagat taatgactct gaagtcgtgg ttgctaaaaa cgcggtccag aactttattg
    21661 cctactgcat ggcagtccgc gccaataacg ccaatcacaa gtgttgcttt cttcatgggt
    21721 aatcctttgt aagacagaga gtaaaataag ttacacacgt cgtatttatg gccgttcata
    21781 acggcacagg tttaatatca caggagtgtc tttatctgac cgtaatgttg caattgatct
    21841 attttcaaaa tgtctttcgg gaaatgattg tttgaagtgt gtttttaaat ttccatgtta
    21901 attcagtgtg ttatgtcaac taatgtcatg agtgttaaat ttttaaaatt aacttgattt
    21961 aatgtattga gataattgtg atctacttct aatttgcatt tttgtgacgt ggttttagcc
    22021 atgagtaaat gcatttcata agatttaagt cgaatttcac tgcaattcat ctctgcttaa
    22081 tgtaaaaata atgttgaaaa taacaaatgg ttaatgattc atgcgaatta tgaccttagt
    22141 tagctctttt aatttttcgt aaaaaagaga ggtacatgaa ggtgctatca gtatgagtaa
    22201 cgttaatcaa aggttttatt acgtctggca ataacgcagg ccatttttcg cgacagggtg
    22261 gatttaatca gtataatcct catacaatag ttcacgatta actgaaccgt taacggggat
    22321 ttggtgttga aatttagaaa tggcggagtc aattaatgaa gacaatatcg aaaaaatgac
    22381 tgtgtcagaa ttaatttcaa ttaatccttc tcgttgttga ccaaaaaaga tatttatctt
    22441 gatttcattt gtactacggc aatcaatatg tagtaatttt tgatcgctga gattttctgt
    22501 ttcataaatg tttttggcga gagtcgggtg tattgatgcc agacatctgc tcagataagc
    22561 catatccttt agggaacacc ccgctgagta gaggatgtca gagaattttt gtgtgtcaac
    22621 tgttgtctct gaagcgtatg ttgcattgac tggaagggta ttgaaagaga tctgagatgg
    22681 tatcattatt taaatctttc cgtgagtaat aaaaattaat ccccaggcat tgtttag
//
LOCUS       DAZKIP010000070        22258 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZKIP010000070 DAZKIP010000000
VERSION     DAZKIP010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.070"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            158..826
                     /locus_tag="ACHOUK_002937"
     CDS             158..826
                     /locus_tag="ACHOUK_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFP9898342.1"
                     /translation="MFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNKIDLLE
                     KQQAQLKSQLETIQKQQTGIISSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKYFILNN
                     GSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEFARWQQ
                     LLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSAENRQK
                     RSKK"
     gene            870..5636
                     /gene="ehaG"
                     /locus_tag="ACHOUK_002938"
     CDS             870..5636
                     /gene="ehaG"
                     /locus_tag="ACHOUK_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HFP9898343.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIAIGAETEAAENATAIGNNAKAKGTNSMAMGFGSLADKVNTIALGNGSQALADNAIA
                     IGQGNKADGVDAIALGNGSQSRGLNTIALGTASNATGDKSLALGSNSSANGINSVALG
                     ADSIADLDNTVSVGNSSLKRKIVNVKNGAIKSDSYDAINGSQLYAISDSVAKRLGGGA
                     AVDVDDGTVTAPTYNLKNGSKNNVGAALAVLDENTLQWDQTKGKYSAAHGTSSPTASV
                     ITDVADGTISASSKDAVNGSQLKATNDDVEANTANIATNTSNIATNTANIATNTTNIT
                     NLTDSVGDLQADALLWNETKKAFSAAHGQDTTSKITNVKDADLTADSTDAVNGSQLKT
                     TNDAVATNTTNIANNTSNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGTET
                     TSKITNVKDGDLTTGSTDAVNGSQLKTTNDAVATNTTNIATNTTNISNLTETVTNLGE
                     DALKWDKDNGVFTAAHGNNTASKITNILDGTVTATSSDAINGSQLYDLSSNIATYFGG
                     NASVNTDGVFTGPTYKIGETNYYNVGDALAAINSSFSTSLGDALLWDATAGKFSAKHG
                     TNGDASVITDVADGEISDSSSDAVNGSQLHGVSSYVVDALGGGAEVNADGTITAPTYT
                     IANADYDNVGDALNAIDTTLDDALLWDADAGENGAFSAAHGKDKTASVITNVANGAIS
                     AASSDAINGSQLYTTNKYIADALGGDAEVNADGTITAPTYTIANAEYNNVGDALDALD
                     DNALLWDETANGGAGAYNASHDGKASIITNVANGSISEDSTDAVNGSQLNATNMMIEQ
                     NTQIINQLAGNTDATYIQENGAGINYVRTNDDGLAFNDASAQGVGATAIGYNSVAKGD
                     SSVAIGQGSYSDVDTGIALGSSSVSSRVIAKGSRDTSITENGVVIGYDTTDGELLGAL
                     SIGDDGKYRQIINVADGSEAHDAVTVRQLQNAIGAVATTPTKYFHANSTEEDSLAVGT
                     DSLAMGAKTIVNGDKGIGIGYGAYVDANALNGIAIGSNAQVIHVNSIAIGNGSTTTRG
                     AQTNYTAYNMDAPQNSVGEFSVGSADGQRQITNVAAGSADTDAVNVGQLKVTDAQVSQ
                     NTQSITNLDNRVTNLDSRVTNIENGIGDIVTTGSTKYFKTNTDGVDASAQGKDSVAIG
                     SGSIAAADNSVALGTGSVATEENTISVGSSTNQRRITNVAAGKNATDAVNVAQLKSSE
                     AGGVRYDTKADGSIDYSNITLGGGNGGTTRISNVSAGVNNNDVVNYAQLKQSVQETKQ
                     YTDQRMVEMDNKLSKTESKLSGGIASAMAMTGLPQAYTPGASMASIGGGTYNGESAVA
                     LGVSMVSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            6004..7659
                     /gene="lldP"
                     /locus_tag="ACHOUK_002939"
     CDS             6004..7659
                     /gene="lldP"
                     /locus_tag="ACHOUK_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFP9898344.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7659..8435
                     /gene="lldR"
                     /locus_tag="ACHOUK_002940"
     CDS             7659..8435
                     /gene="lldR"
                     /locus_tag="ACHOUK_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFP9898345.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTIRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8432..9622
                     /gene="lldD"
                     /locus_tag="ACHOUK_002941"
     CDS             8432..9622
                     /gene="lldD"
                     /locus_tag="ACHOUK_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFP9898346.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9808..10281
                     /gene="trmL"
                     /locus_tag="ACHOUK_002942"
     CDS             9808..10281
                     /gene="trmL"
                     /locus_tag="ACHOUK_002942"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFP9898347.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVNYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10334..11155)
                     /gene="cysE"
                     /locus_tag="ACHOUK_002943"
     CDS             complement(10334..11155)
                     /gene="cysE"
                     /locus_tag="ACHOUK_002943"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFP9898348.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11235..12254)
                     /gene="gpsA"
                     /locus_tag="ACHOUK_002944"
     CDS             complement(11235..12254)
                     /gene="gpsA"
                     /locus_tag="ACHOUK_002944"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFP9898349.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12254..12721)
                     /gene="secB"
                     /locus_tag="ACHOUK_002945"
     CDS             complement(12254..12721)
                     /gene="secB"
                     /locus_tag="ACHOUK_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFP9898350.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12784..13035)
                     /gene="grxC"
                     /locus_tag="ACHOUK_002946"
     CDS             complement(12784..13035)
                     /gene="grxC"
                     /locus_tag="ACHOUK_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFP9898351.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13177..13608)
                     /gene="yibN"
                     /locus_tag="ACHOUK_002947"
     CDS             complement(13177..13608)
                     /gene="yibN"
                     /locus_tag="ACHOUK_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFP9898352.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13853..15397
                     /gene="gpmM"
                     /locus_tag="ACHOUK_002948"
                     /gene_synonym="pgmI"
     CDS             13853..15397
                     /gene="gpmM"
                     /locus_tag="ACHOUK_002948"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFP9898353.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15431..16690
                     /gene="envC"
                     /locus_tag="ACHOUK_002949"
     CDS             15431..16690
                     /gene="envC"
                     /locus_tag="ACHOUK_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFP9898354.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16694..17653
                     /gene="yibQ"
                     /locus_tag="ACHOUK_002950"
     CDS             16694..17653
                     /gene="yibQ"
                     /locus_tag="ACHOUK_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFP9898355.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17640..18675)
                     /gene="waaH"
                     /locus_tag="ACHOUK_002951"
                     /pseudo
     CDS             complement(17640..18675)
                     /gene="waaH"
                     /locus_tag="ACHOUK_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
     gene            complement(18914..19939)
                     /gene="tdh"
                     /locus_tag="ACHOUK_002952"
     CDS             complement(18914..19939)
                     /gene="tdh"
                     /locus_tag="ACHOUK_002952"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFP9898356.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMCSGQSG
                     KVILSWD"
     gene            complement(19949..21145)
                     /gene="kbl"
                     /locus_tag="ACHOUK_002953"
     CDS             complement(19949..21145)
                     /gene="kbl"
                     /locus_tag="ACHOUK_002953"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFP9898357.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANVRQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(21420..22256)
                     /gene="yibB"
                     /locus_tag="ACHOUK_002954"
     CDS             complement(21420..22256)
                     /gene="yibB"
                     /locus_tag="ACHOUK_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HFP9898358.1"
                     /translation="MTAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLAALENEMI
                     IFTSPDLKPRVEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRLEPRQLKN
                     PEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLKIWDFPFD
                     ESKMHLFTIKKGLTVTSQQQVFDFMIGNHVYIIGGAIVGSQHKWKEFYKLVLESQKIT
                     LNNNIVDDDQGIFVMCYYKRPDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQALRIFLS
                     RK"
BASE COUNT         5681 a         5741 c         5614 g         5222 t
ORIGIN      
        1 tacattttta taatgaatgc ttaaaacaat atttaatcga cacacatcac tgtcattaat
       61 gtaaagcaac atagatcacc aataatacaa tggacaactc taccggcatt gatcgctggg
      121 cggaggaaat aaggattttt acatgaacta gaagaagatt tttttcagtg ctgtcactgt
      181 ttcagttctc tgcgcattaa cgggatgtga ctacattgaa gaaggcaagc cagaaagtag
      241 tttattgaaa caacaagaag aacataacaa taaaattgat ctacttgaaa aacaacaagc
      301 acagctaaag agtcaactgg aaactattca aaaacaacaa actggcataa taagcagtac
      361 aaagacttta actcacgtaa ttaaatccgt taaagatcaa caaaacactt ttatttttac
      421 cgagtttaac ccagcaaaaa cgaagtattt catcctgaac aatggttcgg tggctttagc
      481 gggtcgagtg ttatccattg acgccacaga gaatggcagt gttattcata tttcactggt
      541 caacttatta agtacaccta tctcaaatat tggttttaat gcgacatggg gtggtgaaaa
      601 acctgtcgat gccaaagagt ttgcccggtg gcaacaatta cttttcaaca catcaatgaa
      661 atccacattg aaattattac caggtcaatg gcaagacatt aatttgaccc tgaagggtgt
      721 atcgcccaat aacctgggat atctgaagtt agccatcaac atggaaaata ttcagttcga
      781 caatcttccc tctgctgaaa atcggcagaa aagaagcaaa aaataaatgc agttagtacg
      841 cttataacaa ggaatagcga gcatcattaa tgaacaaaat atttaaagtt atctggaacc
      901 ctgcgacagg gaattatact gttaccagcg aaacggcaaa aagccgtggc aagaaatctg
      961 ggcgcagtaa gctgttaatt tctgcgctgg ttgcgggtgg aatgttgtcg tcgtttgggg
     1021 cattggcgaa tgccgggaat gacaacggtc agggtgttga ttacggtagt ggatcagctg
     1081 gcgacggctg ggttgctata ggcaaagggg cgaaagcaaa tacttttatg aacaccagtg
     1141 gttccagtac tgctgtgggt tatgacgcta tagctgaagg ccaatatagc tctgccatcg
     1201 ggtcaaaaac ccatgcgatt ggtggtgcat caatggcctt tggggttagt gcaatatcag
     1261 aaggcgatag aagtatagca ctgggtgcct cttcgtattc attgggccaa tactcaatgg
     1321 ccctcggccg ttattcaaaa gcattgggta aattgtctat tgctatgggg gactcttcca
     1381 aagcggaagg agcaaacgcc attgccctgg gaaatgccac taaagctact gagattatga
     1441 gtattgctct tggcgacacc gccaatgcgt caaaagcgta ttcaatggcg ctgggagcaa
     1501 gtagcgtcgc atctgaagaa aacgctattg cgataggtgc tgagaccgaa gccgctgaaa
     1561 atgcaactgc tattggcaat aatgcgaagg caaaagggac taatagcatg gcaatggggt
     1621 tcggaagcct tgccgataaa gtcaatacta tcgcattagg aaatggcagc caggctctgg
     1681 cagataatgc aatcgccata ggccagggca acaaagctga tggcgtggat gccatcgctc
     1741 tgggtaatgg tagccagtcg agaggcttaa acaccattgc cttaggcaca gccagtaatg
     1801 caactggtga taagagtctt gcgcttggta gtaatagcag tgccaacggt attaactctg
     1861 tcgcgctggg cgcagattcc attgcggatt tagacaatac cgtctctgtc ggcaatagtt
     1921 cattaaaacg caagatcgtt aatgtgaaaa atggcgcgat caagtctgac agttacgatg
     1981 ccattaatgg ttcacagctt tatgccatta gcgactcggt agcaaaaagg cttggaggag
     2041 gggctgcagt agatgttgat gacggtactg ttacagcacc aacctacaat ttaaaaaatg
     2101 gtagcaaaaa taacgtaggg gctgcgctcg ctgtacttga tgaaaacacc ctgcaatggg
     2161 accaaaccaa aggcaaatac agcgctgctc atggtactag tagcccaact gccagcgtaa
     2221 tcaccgatgt tgcggatggc acgatttcag cctccagtaa ggatgcggtt aacggttccc
     2281 aactgaaagc taccaatgac gatgtcgaag ccaacaccgc caatatcgct actaatacca
     2341 gcaacattgc cacgaatacg gcaaatattg ccaccaatac caccaatatc accaacctga
     2401 cggattccgt tggtgacctt caggctgatg ccctgctctg gaacgaaact aaaaaggcat
     2461 tcagtgcagc tcacggccag gataccacca gcaaaatcac caacgttaaa gatgccgacc
     2521 tgacggctga cagcactgat gctgttaacg gctctcagct gaaaaccacc aacgatgctg
     2581 tggcgacgaa taccaccaat atcgccaata acacttccaa tattgccact aacaccacca
     2641 acatctctaa cctgactgag acggtgacta atcttggtga ggatgcgctg aaatgggata
     2701 aggacaatgg tgtattcacg gcagctcatg gcaccgagac caccagcaaa atcaccaacg
     2761 ttaaagatgg cgacctgacg actggcagca ccgatgccgt taacggctct cagctgaaaa
     2821 ccaccaacga tgccgtggcg acgaatacca ccaatatcgc cactaacacc accaacatct
     2881 ctaatctgac tgagacggtg actaatcttg gtgaggatgc gctgaaatgg gataaggaca
     2941 atggtgtctt cactgcagct catggcaaca ataccgccag caaaatcacc aatatcctgg
     3001 acggcacagt cactgcaacc agttccgatg ccattaacgg tagccagctt tatgacttaa
     3061 gcagcaatat cgccacctac ttcggcggca atgcttctgt gaatactgac ggtgtgttta
     3121 ccggtccaac ctacaaaatc ggtgaaacaa attattataa cgtcggcgat gcactggctg
     3181 cgattaactc ctcatttagc acgtctctcg gcgatgctct gctttgggat gccaccgcag
     3241 gtaaattcag tgccaaacac ggtactaatg gtgacgcaag cgtgatcact gatgtcgcag
     3301 atggtgaaat ttcagactcc agttctgacg cagtaaacgg ctcacaactc cacggcgtga
     3361 gcagttatgt tgttgatgcg ctggggggtg gtgccgaagt caatgcagac ggcaccatca
     3421 ctgcgccgac gtacaccatt gctaatgctg attacgataa tgtcggtgat gccctgaatg
     3481 ctatcgatac cactcttgac gacgctctgc tctgggatgc ggacgccggt gaaaatggtg
     3541 catttagcgc cgctcacgga aaagataaaa ctgccagtgt aatcactaac gtcgctaacg
     3601 gtgcaatctc tgctgccagc agcgacgcga ttaacggctc acaactctat accaccaata
     3661 agtacatcgc tgatgcgctg ggtggtgacg cagaagtcaa cgctgacggc accatcaccg
     3721 caccgactta caccattgcg aacgccgagt acaacaacgt cggtgacgcc ctggatgcgc
     3781 ttgatgataa cgccctgctg tgggatgaga ctgccaatgg cggtgctgga gcctacaatg
     3841 ccagccatga cggtaaagcc agcatcatca ctaatgtcgc taatggcagt attagtgagg
     3901 acagtaccga tgcagtgaac ggttctcagt tgaatgcgac gaatatgatg attgagcaga
     3961 acacccaaat tatcaatcag ctcgctggta acaccgacgc aacctatatc caagaaaacg
     4021 gtgcgggtat taactatgtg cgtactaacg acgacggctt agcgttcaac gacgccagcg
     4081 cacagggtgt tggcgctaca gctataggtt ataactctgt cgccaaaggc gatagcagcg
     4141 tagctattgg tcagggcagc tacagcgacg ttgatacggg tatcgccctg ggtagcagct
     4201 ctgtttccag ccgagtgatt gccaaaggct cccgtgacac cagcataacg gaaaatggcg
     4261 ttgttattgg ttacgacacc acggatggcg aactgctcgg tgcattgtct atcggtgatg
     4321 acggtaaata tcgtcaaatc atcaacgtag ccgatggttc cgaagcccat gacgccgtta
     4381 cggttcgtca attgcagaat gcgattggtg cggtcgcaac cacgccgact aaatacttcc
     4441 acgctaattc aacggaagaa gattcactgg cagtgggaac tgactcgctg gcaatgggtg
     4501 cgaaaaccat cgtgaatggc gataaaggta ttggtatcgg ttatggtgcc tacgtggacg
     4561 cgaatgcact taacggcatt gccattggta gcaatgcgca agtcattcat gtcaacagta
     4621 ttgcgatagg taatggttct acgaccactc gtggcgctca aaccaattat accgcctaca
     4681 acatggacgc accgcagaac tctgtcggtg aattctcagt cggtagtgcg gatggtcaac
     4741 gtcagatcac taacgtcgca gcaggttcgg ctgataccga tgcggtcaac gtgggtcagt
     4801 tgaaagtaac ggatgcgcag gtttcccaga atacccagag cattactaac ctggataatc
     4861 gggtaacgaa tcttgattca cgcgtcacca atatcgaaaa cggtattggc gatatcgtca
     4921 ccaccggtag caccaagtac ttcaagacca ataccgatgg tgtagatgcc agcgcgcagg
     4981 gtaaagatag cgtcgcgatt ggttccggct ccattgctgc cgctgacaac agcgtcgctc
     5041 tgggtacagg gtctgtggca accgaagaaa atacgatctc tgtaggttcc tctactaacc
     5101 aacgtcgtat caccaacgta gctgcaggta aaaatgctac cgatgctgtt aacgtggcac
     5161 agttgaagtc ttccgaagct ggcggtgtac gttacgacac caaagctgat ggttctatcg
     5221 actatagcaa tatcaccctc ggtggcggca acggcggtac gactcgtatc agcaacgtct
     5281 ccgctggcgt caacaacaac gacgtggtga attacgcgca gttgaagcaa agcgtgcagg
     5341 aaacgaagca atacaccgat cagcgaatgg ttgagatgga taacaaactg tctaaaactg
     5401 aaagcaagtt gagcggtggt atcgcttctg caatggcaat gaccggtctg ccgcaggctt
     5461 acactccagg tgccagcatg gcctctattg gtggcggtac ttacaacggt gaatcggcag
     5521 ttgctttagg tgtatcgatg gtgagcgcca atggtcgttg ggtctacaaa ttacaaggta
     5581 gtaccaatag ccagggtgaa tactccgccg cactcggtgc cggtattcag tggtaatgat
     5641 ccattaagtt agtgtgacta agggcaaacg ccaaattgcc tgatgcgtta cgcgcatcag
     5701 gcaagataag gcgctccggg tcaacaatct aagggcaatt ctctgatgag gattgccctt
     5761 ttctttacca gacatctccc cccacaagaa ttggccctac caattcttcg cttatctgac
     5821 ctctggttca caattcccca attaaaactc acatcaatat tgccaataca taacatttag
     5881 ttaaccattc attgtcatta tccctacaca acacaattgg cagtgccact tttacacaac
     5941 gtgtgacaag gagatgagca acagactcat tacacgatgt gcgtggactc caggagacct
     6001 gcaatgaatc tctggcaaca aaactacgat cccgccggga atatctggct ttccagtttg
     6061 atagcatcgc ttcccatcct gtttttcttc tttgcgctga ttaagctcaa actgaaagga
     6121 tacgtcgccg cctcatggac ggtagcaatc gcccttgccg tggctttgct gttctataaa
     6181 atgccggtcg ctaacgcgct ggcctcggtg gtttatggct tcttctatgg tttgtggccc
     6241 atcgcgtgga tcattattgc agcagtgttc gtctataaga tctcggtgaa aaccgggcag
     6301 tttgacatca ttcgctcgtc tattctttcg ataacccctg accagcgtct gcaaatgctg
     6361 atcgtcggtt tctgtttcgg cgctttcctt gaaggagccg caggctttgg cgcaccggta
     6421 gcaattaccg ccgcattgct ggtcggcctg ggttttaaac cgctgtacgc cgccggactg
     6481 tgcctgattg ttaacaccgc gccagtggca tttggcgcga tgggcattcc aatcctggtt
     6541 gccggacaag taacaggcat cgacagcttt gagattggcc agatggtggg gcggcagcta
     6601 ccgtttatga ccattatcgt gctgttctgg atcatggcga ttatggacgg ctggcgcggt
     6661 atcaaggaga cgtggcctgc ggtcgtggtt gcgggcggct cgtttgccat cgctcagtac
     6721 cttagctcta acttcattgg gccggagctg ccggacatta tctcttcgct ggtatcactg
     6781 ctctgcctga cgctgttcct caaacgctgg cagccagtgc gcgtattccg ttttggtgat
     6841 ttgggggcgt cacaggttga tatgacgcta gcccacaccg gttacactgc gggtcaggtg
     6901 ttacgtgcct ggacaccgtt cctgttcctg acagctaccg taacactgtg gagtatcccg
     6961 ccgtttaaag ccctgttcgc atcgggtggc gcgctgtatg agtgggtgat caacattccg
     7021 gtgccgtacc tcgataaact ggttgcccgt atgccgccag tggtcagcga ggctacagcc
     7081 tatgccgccg tgtttaagtt tgactggttc tctgccaccg gcaccgccat tctgtttgct
     7141 gcactgctct cgattgtctg gctgaagatg aaaccgtctg acgctatcag caccttcggc
     7201 agcacgctga aagaactggc tctacccatc tactccatcg gcatggtgct ggcgttcgcc
     7261 tttatctcga actattccgg actgtcatca acactggcac tggcactggc gcacaccggt
     7321 catgcattca ccttcttctc gccgttcctc ggctggctgg gggtattcct gaccgggtcg
     7381 gatacctcat ctaacgccct gttcgccgcg ctgcaagcca ccgcagcaca acaaattggc
     7441 gtctctgatc tgttgctggt tgccgccaat accaccggtg gcgtcaccgg taagatgatc
     7501 tccccgcaat ctatcgctat cgcctgtgcg gcggtaggcc tggtgggcaa agagtctgat
     7561 ttgttccgct ttactgtcaa acacagcctg atcttcacct gtatggtggg cgtgatcacc
     7621 acgcttcagg cttatgtctt aacgtggatg attccttaat gattgtttta cccagacgcc
     7681 tgtcagacga ggttgccgat cgtgtgcggg cgctgattga tgaaaaaaac ctggaagcgg
     7741 gcatgaagtt gcccgctgag cgccaactgg cgatgcaact cggcgtatca cgtaattcac
     7801 tacgcgaggc gctggcaaaa ctggtgagcg aaggcgtgct gctcagtcga cgcggcggcg
     7861 ggacgtttat tcgctggcgt catgacacat ggtcggagca aaacatcgtc cagccgctaa
     7921 aaacgctgat ggccgatgat ccggattaca gtttcgatat tctggaagcc cgctacgcca
     7981 ttgaagccag caccgcatgg catgcggcaa tgcgcgccac acctggcgac aaagaaaaga
     8041 ttcagctttg ctttgaagca acgctaagtg aagacccgga tatcgcctca caagcggacg
     8101 ttcgttttca tctggcgatt gccgaagcct cacataacat cgtgctgctg caaaccatac
     8161 gcggtttctt cgatgtcctg caatcctcag tgaagcatag ccgtcagcgg atgtatctgg
     8221 tgccaccggt tttttcacaa ctgaccgaac aacatcaggc tgtcattgac gccatttttg
     8281 ctggtgatgc cgacggggcg cgtaaagcaa tgatggcgca ccttagtttt gttcacacca
     8341 ccatgaaacg attcgatgaa gatcaggctc gccacgcacg gattacccgc ctgcccggtg
     8401 agcataatga gcattcgagg gagaaaaacg catgattatt tccgcagcca gcgattatcg
     8461 cgccgcagcg caacgcattc tgccgccgtt cctgttccac tatatggatg gtggtgcata
     8521 ttctgaatac acgctgcgcc gcaacgtgga agatttgtca gaagtggcac tgcgccagcg
     8581 tattctgaaa aacatgtctg acttaagcct ggaaacgacg ctgtttaatg agaaattgtc
     8641 gatgccggta gcactggcac cggtgggttt gtgtggcatg tatgcgcgtc gtggcgaagt
     8701 tcaggcagcc aaagcggcgg acgcgcatgg cattccgttt actctctcaa cggtttccgt
     8761 ttgcccgatt gaagaagtcg cgccagccat caagcgccca atgtggttcc agctttatgt
     8821 actgcgcgat cgcggcttta tgcgtaacgc gctggagcga gcaaaagcag cgggttgttc
     8881 gacgctggtt ttcaccgtgg atatgccgac accgggagcg cgttatcgtg atgcgcattc
     8941 tgggatgagc ggcccaaatg cggcaatgcg ccgctacttg caagcggtga cgcatccgca
     9001 atgggcgtgg gatgtgggcc tgaacggtcg tccgcatgat ttaggtaata tttcggctta
     9061 cctcggcaaa ccgaccggac tggaagatta catcggctgg ctggggaata acttcgatcc
     9121 gtccatctca tggaaagacc ttgagtggat ccgcgatttc tgggatggcc cgatggtgat
     9181 caaagggatc ctcgatccgg aagatgcgcg cgatgcagta cgttttggtg ctgatgggat
     9241 tgtagtttct aaccacggtg gccgccagct ggacggtgta ctctcttccg ctcgtgcgtt
     9301 gcccgctatt gcagatgcgg tgaaaggtga tatcgccatt ctggcggata gcggaattcg
     9361 taacgggctt gatgtcgtgc gtatgattgc gctcggtgcc gacaccgtac tgctgggtcg
     9421 tgctttcctg tatgcgctgg caacagcggg ccaggcgggt gtagctaacc tgctaaatct
     9481 gatcgagaaa gagatgaaag tggcaatgac gctgactggc gcgaaatcga tcagtgaaat
     9541 tacgcaagat tcgctggtgc aggggctggg taaagaattg cctacggcac tggctccgat
     9601 ggcgaaaggg aatgcggcat agtcgtttga gccccctcac cctaaccctc tccctcaggg
     9661 agtggggacc gttcggcgct gtatgtactc cctcactctg aaacgacacc gcactctttt
     9721 tttctccctc gcccctccgg ggtgagggga aaaggccgca ctgctcacca attttctgct
     9781 atcctgcccc cgcactaagg gggcagtatg ctaaacatcg tactttacga accagaaatt
     9841 ccgccaaata ctggcaatat catccgtctt tgcgctaata ccggctttcg tctgcatatc
     9901 atcgaaccga tgggatttgc ctgggacgat aagcgcctgc gccgcgcggg gctggactat
     9961 cacgagttta ccgccgttac gcgtcatcat gactttcgcg cgttcctcga agcagaaaat
    10021 ccccagcgcc tgtttgccct caccacgaaa ggtacgcctg ctcacagcgc cgtaaactat
    10081 caggatggcg attatctgat gttcggcccg gaaacacgcg gcctgccagc gagcattctt
    10141 gatgccctgc ccgctgaaca aaaaattcgc attccgatgg tgccggacag ccgcagcatg
    10201 aacctgtcca atgcggtgtc agttgtggtg tatgaagcct ggcggcagct gggatatccg
    10261 ggagcgttgt tgagagatta gttactgtat gccggataga tgattacatc gcatccggca
    10321 cgatcacagg acattagatc ccatccccat actcaaatgt atggttaata ccgttgaaat
    10381 gctggtccat atccattgat ggcttatcgc tgtctggttt accgacaata cgagccggaa
    10441 cgccagcggc ggttgtatgc ggcggcaccg gttgcagcac cacggaacct gcgccaatct
    10501 tcgcgccgcg accaacttca atattgccga ggattttcgc gcccgcgcca atcatcacac
    10561 cttcacgaat tttcgggtga cggtcaccac cagatttacc cgtaccgcca agcgtcacag
    10621 attgcagaat cgatacgtcg ttttcaatta ccgccgtttc acccacaacg atgcccgtgg
    10681 cgtggtcgag cataatgcca cggccaattt ttgctgccgg gtgaatatcg acctggaacg
    10741 tcacagaaac ctggttttgc agaaagattg ccaatgcgcg acgcccctga ttccacaacc
    10801 agtgaccgat gcgataggcc tgcaaggcat gaaaaccctt caggtataac aacggggttg
    10861 agtatttatc gactgccggg tcgcgggtac gcaccgcctg aatatcacag gccgcagagg
    10921 cgatcatttc cgggtcagcg gcgtaggctt cttccaccac ttcacggata gcaatagcag
    10981 gcataattgg cgatgacagc ttgttcgcca gcatgtagct cagtgcacta ccaaggtttt
    11041 cgtgcttgag tagcgtcgcg tggtaaaaac tggccagcat tggctcacag tccgccagcg
    11101 ttctggcttc ggctttaata ttgttccaga caatttccag ttcttcacac gacattgctt
    11161 actccacacg atgagataat gaccggcccg ttctgcgcgg gccgggtcat agcggtaaca
    11221 aaggttccct ggggttagtg gctgctgcgc tcgtccttgc gtgcacgacc taataacgtc
    11281 aatgctgcct cgcgcgcgtt ttttccgcaa tataatactt gataaatttc ctcggttatt
    11341 ggcatttcta cgccgaagcg atgcgccagt tcgcggactt ctttcgtatt gcggtagcct
    11401 tccaccacct gaccaatctt ctcctgcgcg ctttgtacat ccatgccctg accgagcatc
    11461 atgccaaaac ggcggttacg cgactggttg tcggtacaag taagcacaag atcgcccagc
    11521 cccgccatgc ccataaaggt ggcaggatcg gcacccagcg ccgcgccaag acgcgacatt
    11581 tcagccagcc cacgggtgat cagtgccgta cgcgcattcg caccaaaacc gataccgtcg
    11641 gacatccccg cgccaatggc aatgacgttt ttcaccgcac cgccaagctg cacgccaatg
    11701 aaatccggat tgctgtaaac gcggaaactt ttgccgcagt gcagcagctg ctggagatca
    11761 tcggcaaagg tctgatcggt cgaggccagc gaaatagctg tcggtaaacc tgccgccagt
    11821 tctttcgcaa acgttgggcc agagataacc gccagcggaa tttgatcgcc taaggcctca
    11881 cgcgccacgt cctgtaacag acgtccggtt tccgcttcca gccctttggt cgcccacacc
    11941 agacgcgcat caggacgcat cagcggttta atctggcgca gcacttcacc aaagacatgg
    12001 ctgggtacga cgacgagaat attacggctg gctgccagcg cagtggcgag atcgctttca
    12061 agatggagcg tatcgggaaa aggcacatcg gggagaaacg cggcgttaca gcggtcgcgt
    12121 tcaagcgttg cgatatgttc agggtcatgg ccccagagga caacctcgtg gccatttctt
    12181 gccagggtga tggcaagagc ggtgccgtac gagccggcac cgatcacagt cattgaagca
    12241 ttacgttggt tcatcaggca tcctgatgtt cttcagtacc ttcgccagcc tgctgctgca
    12301 aatagttcat gaacagcgca tcgaagttaa ccggcgcaag gttcagttgc gggaatgtac
    12361 cgcgggatac catgctggtg atgcactcac gagcatacgg gaacagaatg ttcgggcagt
    12421 atgcgcccag gcaatgtgcc atctgggtgc cttcgatacc cgcgatggag aaaataccgc
    12481 cctgctgaac ttcacacagg aacgcggttt cttcgcccag ggaggccgtt acggtaacac
    12541 gcagtaccac ttcgtatacg tcatctgcca gttgggaaga tgccgtatcc agatcaagtt
    12601 taacttctgg ttgccaatct ttctggaaaa cgtgcggcgc gttcggcgct tcgaaagaga
    12661 tatccttggt ataaatacgt tggatctgga aagtcatttc agtgttgttt tgttctgaca
    12721 tgtgtagaaa acccttaagt gttgtcctta aatactgcgc aatgtaatgc caacgttcac
    12781 acgttatttc agcaggggat ccagtccacc acgtgcatcc aatgcataca agtcatcaca
    12841 gccgccaatg tgctgtgcgt caataaaaat ctggggaacc gtggtgcgac cgctgcgttt
    12901 gatcatctct tcacgcttgg cggcattgcc atcgatcggc aactcctgaa aactcacgcc
    12961 cttgctgctc agcagtgctt ttgcacgatg gcaatacggg caggtttctt tggtatagat
    13021 ttcaacattg gccataattt agctcctgtt tttttaccct gaaatttcat gttgcagggc
    13081 ggctgcaaaa tggagtgtcc ccagaagctt ataacaataa gtcgctgggg gcctaattgc
    13141 agctaacggc ctgcatcatg aaagacgaca ggtaaattat ttgccgcgca ccaaaggcaa
    13201 gttttcgcca gcccagccag cgacgccttc tttcagcacg aatacttgcg caaaaccagc
    13261 tttcgtcagt gcgtttgcag gctcctggca ctgcatgcca gaaccgtcta ccacgataac
    13321 cggtttgtct ttgtgcttct caagctcacc aacattgttg gctttgattt cgctcggcaa
    13381 cagattgata gaacctgcga tatggccttt acggaagtca tcacgctgac gtaaatccac
    13441 aaccacagcg tcttctttgt tgatcagacg cgtagcttca ccacgagtaa tcaccttcac
    13501 tttcgaggtc aggctcttaa acgtagtcac aagaaccgcc accagtaacg cgatccaggc
    13561 gatactcagt atgggatgac ggccaacaaa ttgcataatt tcttgcatgg ggggtaacaa
    13621 ctcccgacgt agtgattaaa aaaaccagga aaggagtata cctgcgcggt gcggcaaata
    13681 cagccagcgc gttaactgga atgcaatttt gcggggcacg acgaaaaaaa agcgcaaatc
    13741 tggcatcggc ttgccgcgag cggtcgtata ttttgatctt cagaggctat tttatcgatt
    13801 cagctgtagt aaaattacgc aaattttgac tcttgagtat gaggttgtcg caatgtcggt
    13861 ttctaaaaaa cctatggtac tggtgattct ggatggctat ggctatcgtg aagaacagca
    13921 ggataacgcc atttttagtg ctaaaacccc ggtaatggat gcactgtggg ccaatcgtcc
    13981 gcataccctg atcgacgctt ccggtctgga agtcggtctg cctgaccgtc agatgggtaa
    14041 ctccgaagta ggccacgtta acctgggtgc cggccgcatc gtgtatcagg acctgactcg
    14101 tctggacgtt gaaatcaaag atcgcgcttt ctttgctaat ccagtgctga ctggcgcggt
    14161 agataaagcg aaaaacgcag gtaaagcagt acacattatg ggtctgctct ccgcaggcgg
    14221 cgtacacagc cacgaagatc acatcatggc gatggtagaa ctggcagctg aacgcggcgc
    14281 agaaaaaatc tacctgcacg cattccttga cggtcgcgac accccgccgc gcagtgctga
    14341 atcctcgctg aaaaaatttg aagagaaatt tgctgcgctg ggtaaaggcc gcgtagcgtc
    14401 catcattggt cgttactacg cgatggaccg cgataaccgt tgggatcgcg ttgaaaaagc
    14461 ctatgacctg ctgactctgg cgcagggtga gttccaggcc gatactgccg ttgctggttt
    14521 gcaggcagct tatgctcgcg acgaaaacga cgagttcgtg aaagcgaccg ttattcgtgc
    14581 agaaggtcag ccagatgcgg caatggaaga cggcgatgcg ctgattttca tgaacttccg
    14641 tgctgaccgc gcgcgcgaaa tcactcgtgc attcgtaaac gcagatttcg acggcttcgc
    14701 gcgtaagaaa gtggttaacg tcgatttcgt gatgctgacc gaatacgccg ctgacatcaa
    14761 aactgcggtt gcttacccac ccgcttccct ggttaacacc ttcggcgagt ggatggcgaa
    14821 aaacgacaaa actcagttgc gtatttccga aaccgaaaaa tatgcccacg ttactttctt
    14881 cttcaacggt ggcgtagaag agtcgttcaa aggcgaagat cgcattctga tcaactcacc
    14941 gaaagtggct acctacgatc tgcaaccgga aatgagctcc gcagagctga ccgaaaaact
    15001 ggttgcggcc atcaagagcg gcaaatacga caccattatc tgtaactatc cgaacggcga
    15061 catggtaggt cacaccgggg tgatggaagc ggcggttaaa gcggttgaag cactggatca
    15121 ctgcgtggaa gaagtcgcga aagcggttga gtccgtgggt ggacaactgc tgatcaccgc
    15181 tgaccacggt aacgctgaac agatgcgcga tccggcaacg ggtcaggcac acacggcaca
    15241 caccaacctg ccagttccgc tgatttacgt tggtgataag aacgtgaaag cggttgcagg
    15301 cggcaaactt tctgacatcg cgccgaccat gttgtcgctg atgggtatgg aaatcccgca
    15361 agagatgact ggtaagccgc tgttcatcgt ggaataatcc ctccccatga ggggaaaggc
    15421 gattaatacc atgacacggg ccgtgaaacc gcgcaggttt gcaatcaggc ccatcatcta
    15481 cgccagcgtt cttagcgctg gcgtattgtt gtgcgccttt tccgcccacg cggatgagcg
    15541 tgaccaactc aaatctattc aggccgatat cgccgcaaaa gagcgcgcgg tacgccaaaa
    15601 gcaacaacaa cgcgcaagcc tgctcgcaca attgaaaaag caggaagaag cgatctctga
    15661 agccacccgt aagctgcgcg aaacgcaaaa cacgctcaat caactcaata aacagattga
    15721 tgagatgaac gcgtcgattg ccaaactgga gcagcaaaaa gccgcccagg agcgcagcct
    15781 cgccgcacaa ctggatgccg cattccgtca gggcgagcat accggtattc agctgattct
    15841 cagcggtgaa gaaagccagc gtggacagcg tttacaggct tatttcggct atctcaacca
    15901 ggcgcgacaa gaaaccattg cccagttgaa gcaaacgcgt gaagaagtcg ccatgcagcg
    15961 tgctgaactg gaagagaaac agagcgagca acaaacgctg ttatatgagc agcgcgccca
    16021 acaggcgaaa ctgactcagg cgctgaacga gcgtaaaaag acgctggcag ggctggagtc
    16081 ttccatccag caaggtcagc aacagttgag cgagctgcgc gccaacgaat cccgtctgcg
    16141 taacagcatt gcccgtgcgg aagccgcggc gaaagcgcgt gcagaacgag aagcacgtga
    16201 ggcccaggcg gttcgcgacc gccagaaaga agcgacgcgc aaaggcacca cctacaaacc
    16261 gaccgaaagc gaaaaatcgc tgatgtcccg aactggtggc ctgggggcgc cgcgtggtca
    16321 ggcattctgg ccggttcgcg ggccgacgct gcatcgctat ggtgaacagc tacagggcga
    16381 actacgctgg aaaggaatgg ttatcggtgc ctctgaaggt actgaagtta aagcgattgc
    16441 cgatggtcgg gtgattctgg ctgactggct gcaaggttac ggtctggtgg tggtggttga
    16501 gcacggtaaa ggcgacatga gtctttacgg ctataatcag agcgcactgg tgagcgttgg
    16561 ttcgcaggtt cgcgcgggcc agccaattgc actggtgggc agcagtggcg gtcagggtcg
    16621 gccttcactc tatttcgaaa ttcgccgcca aggtcaggcg gtcaatccac agccgtggtt
    16681 gggaagataa gttttgtttc catttcgtcg taacgttctt gcatttgccg ctctgttggc
    16741 gctctcctcc cccgtacttg ctggcaaact tgccatcgtc attgatgatt ttgggtatcg
    16801 cccgcacaac gaaaaccagg tgctggcgat gccctccgct atctccgtcg ctgtattacc
    16861 cgattcaccg cacgccagag agatggcgac caaagcgcat aacagcgggc acgaagtgtt
    16921 gattcatctc ccgatggcac cactgagtaa acagccgctg gagaaaaata cgctgcgccc
    16981 ggagatgagc agcgacgaaa ttgagcgcat tattcgtagt gcggtcaata acgtgcccta
    17041 tgccgtgggg atcaacaacc acatgggtag caagatgacc tctaacctgt ttggtatgca
    17101 gaaagtgatg caggcactgg agcgttacaa tctttacttc ctcgacagcg taaccatcgg
    17161 taatacccag gcgatgcgcg ccgcgcaagg tactggcgtg aaggtgatca aacggaaggt
    17221 gttcctcgac gattcgcaga acgaagcgga catccgtgtg caatttaatc gcgcaattga
    17281 tctggcgcgt cgcaacggtt caaccattgc cattgggcat cctcaccctt caacggtacg
    17341 cgtgctacaa cagatggttt ataacctgcc gccagacatt acgctggtga aagccagcag
    17401 cttgctgaat gaaccgcagg ttgatacttc tacaccgccg aaaaacgctg tgcctgatgc
    17461 accgcgtaat ccgttccgtg gcgtgaagct gtgcaaaccg aagaaaccga tagaacctgt
    17521 ttatgctaat cgcttctttg aagtattaag cgaaagcatc agccagagca cgctgatcgt
    17581 ttacttccag catcagtggc aaggctgggg caaacagccc gaagcggcga agtttaacgc
    17641 tagcgcaaat taaaggcgct acgcgtaatg cgatggtgcg accgcgtttt tttcgcgcca
    17701 ttgccataag cggaaagacc acagtaacgc ctggtaaccg accttcacgc tgcgcacatt
    17761 ggtaatcagg cgcttataca taccggaagt aaatatctcg gcaatcatcc gttggcgggt
    17821 aagaatatcc ggctctttgc gcaccgcatg gcaaacacgc aatgcttcgt aagttatttg
    17881 ctgatgaaat tccggataaa tcataatttt gtcggcataa tttcgattta atttctccag
    17941 caggcgggta atcttaatat agtgacgttg ataattaagg tttttattcc cttgtctatg
    18001 caaccgactc actgacgtat tatgcagata atatttatat aatgattgct cggtatatcg
    18061 cgcacgcagc gcgttaaaca tgaattctgt tgtccagaca atatcctgat gatgtaatcc
    18121 ggcaataaat ttaatgttat ttttaacaat aacatcacgg cgataaaccc ccatccagac
    18181 aacgtgagtc caacggcgcg aagaaagccc catccgcagc cagtccgggc cggttaatac
    18241 accggttgag cgaaggcgat cgctggggat ggattgccag gtttctcccg tttcacgaaa
    18301 agaccagtca gcgttgcact gcgccacgtc gaggtcgtcc tctaacgcca tggtcatcag
    18361 cgtttcgtac atggtgggat agacctcatc gtcagcatcg acaaaagcga catatttgcc
    18421 cgtcgccacc tcgatcccac gattacgcgc taccgatgcg ccagcattcg cctgatgcaa
    18481 caaacgaacg tgcggatagt tttctgcgta atgctttgct atttcaacag aattatccgt
    18541 tgaaccatcg ttaataataa tgatttccag agcagtccag gtttgcgtaa ttaaagattc
    18601 catacaagtg cggaaatcat cgcccgcatt atataacgga ataataacac taagtttatt
    18661 ggtgctgttc atcataaatt accgacgacc tctgaagtta tcctgacgtt ttacaggctt
    18721 tgtattaaga aactattaag cctgtgagat attattttta caggtgagag agtcacttca
    18781 gggtagagag ataatgagaa cagctatata aaagatcagc gcaattacga actctttatt
    18841 acactgacag gattttacat atttaacaga ttatcctcag gcaaaagggc tggaatacca
    18901 gccctttttc gtgttaatcc cagctcagaa taactttccc ggactggccc gaacacatag
    18961 cgtcaaagcc cttctggaaa tcatcgatag agaaacgatg ggtaatgatc ggcgagagat
    19021 cgaggccaga ctgaatcagc gccgccatct tgtaccaggt ttcaaacatc tcacgaccgt
    19081 aaatgccttt aatgaacaag cctttaaaga tcactttggt ccagtcgata gacatgtcag
    19141 acggtggaat acccagcatc gcaatacgac cgccgtggtt catggtatcg agcatggtac
    19201 gaaacgctgg cggcgcgccg gacatttcca gaccgacatc aaagccttcg gtcatgccca
    19261 gttcagccat cacatcatta aggttttctt tcgcgacgtt aaccgcacgg gtgataccca
    19321 ttttgcgcgc cagctcaagg cggtattcgt taacatcggt gatcaccaca ttgcgtgcac
    19381 caacgtgttt cgccaccgcc gcagccataa tacctatcgg acctgcacca gaaaccagca
    19441 catcttcgcc caccagatcg aacgacagcg cggtatgcac ggcgttaccg aagggatcaa
    19501 aaatggaagc caggtcgtcg gagatattgt cggggatttt gaaagcgttg aacgccggaa
    19561 tcaccagata ttcggcgaag cagcccggac ggttaacacc gacaccaatg gtgttgcggc
    19621 acagatgggt acgaccgcca cgacagttgc gacagtgacc gcaggtgata tggccttcgc
    19681 cagaaacgcg atcgccgatt ttgaagcctt tcacttcctg accaatacct accacttcac
    19741 cgacatattc atggcctacg accatcggca ccgggatggt tttttgcgac cactcatccc
    19801 agttatagat gtgaacgtca gtcccgcaga tggctgtttt acggatttta atcagcagat
    19861 cgttatgccc gagttccggt acaggaacgt cggtcatcca gatgccctct tccgctttca
    19921 gtttggataa cgctttcatc tcacatcctc aggcgataac gcccagttgt ttaccaatac
    19981 gcgtgaatgc ttctactgca cgcgtaattt gctcaggggt atgcgccgca gacatctggg
    20041 tacgaatacg cgcctgacct ttcggaacga ccggatagaa gaaaccggtg acgtaaatgc
    20101 cctctttttg cagctcacgg gcaaatttct gcgccactac tgcatcgcca agcatgaccg
    20161 gaataatggc atgatcggct cccgccaggg taaagcccgc cgccgacatt tgctcacgga
    20221 actggcgcac gttcgcccac agacggtcac gcagttcgct gcccgcttcg accatctcca
    20281 gtactttgat ggacgcggca acaatggccg gtgccagcga gttggagaac aggtacggac
    20341 gagaacgctg gcgcagccac tcaaccacct ctttgcgcgc cgcggtataa ccaccagaag
    20401 ccccgcccag cgctttacca agcgtaccgg tgataatatc gacccggccc atcacatcgc
    20461 agtattcatg ggaaccacga ccattttcac cgacaaaacc gaccgcgtgg gagtcatcca
    20521 ccatcaccag ggcatcgtat ttatctgcca gatcgcaaac gcctttcagg ttggcaatca
    20581 cgccgtccat tgagaacaca ccatcggtgg caatcagcac atgacgcgca ccggcttcac
    20641 gcgcttcttt cagacgtgct tccagctcct gcatatcgtt gttggcatag cgatagcgtt
    20701 tagctttgca cagacgcaca ccatcaataa tagacgcgtg gttcagtgcg tcggagataa
    20761 tggcgtcttc cgcacccagc agcgtttcaa acagtccacc gttagcatca aagcaggaag
    20821 agtagagaat cgcatcttcc atccccagga atgccgccag tttttgttca agctctttat
    20881 ggctgtcctg agtgccgcaa ataaaacgca ccgaagccat gccgaaaccg tgagaatcca
    20941 ttcccgcctt tgctgccgca atcagatccg gatgattcgc cagcccgaga tagttgttgg
    21001 cacaaaagtt aatgacgtgg cttccatcag ccacagtgat atctgcttgc tgcgcagacg
    21061 taataatgcg ctcttcttta aacaaccctt ccgcccgtgc ggtttccaga tcgttggtta
    21121 actgctgata aaattctcca cgcattgcga ttctccagac agggcaaatt ccagcacata
    21181 ttacccaaac ttataggtcg cgacgagatg acgcgttaac tcttctgcaa aattcaggat
    21241 aaatgacgtg taacttgatg tctctcaaca cctacagata gtagaaaaac tgtacagttt
    21301 tggctgggca taatgatgat tgattttact gcattgatat aaaaagaaaa aatgatgcca
    21361 aagcatcatt tttcctatct cagttggtat caaaacgcta cccactaatg gagggaaacc
    21421 tattttcttg aaaggaaaat tctcagcgcc tgcatttttg cccctaaagt gttactcctg
    21481 aagcagcgaa acaaatcgaa ccattttcct ctccccagat agtttaggtt gaagagatcg
    21541 gggcgtttgt aatagcacat gacaaaaatg ccctggtcat catccacaat gttattattt
    21601 agtgttattt tttgactttc cagaactaat ttatagaatt ctttccattt atgctgtgaa
    21661 ccaacaattg cgcccccaat aatatataca tgattgccaa tcatgaaatc aaaaacctgt
    21721 tgttgagatg tgacagtaag cccttttttt atagtgaaaa gatgcatctt actttcatca
    21781 aatgggaaat cccaaatttt caatcctcga gttacatttg gcttacggca atagccaaaa
    21841 tctatccacg caacaagagg cgttttcact aagcccatgt tgatagcttt atttacaaaa
    21901 taggccttaa ggttacatac caaaacatac tctggtgacc agtactctgg gttttttaat
    21961 tgtcgaggct caagtctatt tgtaaacgac tcatcttttt gaattttttc gatacgactt
    22021 ctgatatatc taaatttctt ttttatatcg ataacgataa ccgttgttgg ttttccgttg
    22081 cgaatcgctt caacccttgg tttcaaatcg ggagaagtga aaataatcat ctcattttca
    22141 agtgcggcca gacgctcaaa gtaactaaaa taaacatcaa ctgaacgagc tagtttttca
    22201 cggaaccctt tattagcagt ccaatcccct ctaccaatgt caaaatatgc agtgataa
//
LOCUS       DAZKIP010000071        21397 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZKIP010000071 DAZKIP010000000
VERSION     DAZKIP010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.071"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            16..1530
                     /gene="caiT"
                     /locus_tag="ACHOUK_002955"
     CDS             16..1530
                     /gene="caiT"
                     /locus_tag="ACHOUK_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFP9898359.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFLWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1561..2703
                     /gene="caiA"
                     /locus_tag="ACHOUK_002956"
     CDS             1561..2703
                     /gene="caiA"
                     /locus_tag="ACHOUK_002956"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFP9898360.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            2832..4049
                     /gene="caiB"
                     /locus_tag="ACHOUK_002957"
     CDS             2832..4049
                     /gene="caiB"
                     /locus_tag="ACHOUK_002957"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFP9898361.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKARETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4123..5676
                     /gene="caiC"
                     /locus_tag="ACHOUK_002958"
     CDS             4123..5676
                     /gene="caiC"
                     /locus_tag="ACHOUK_002958"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFP9898362.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDTFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYDAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFIDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5785..6570
                     /gene="caiD"
                     /locus_tag="ACHOUK_002959"
     CDS             5785..6570
                     /gene="caiD"
                     /locus_tag="ACHOUK_002959"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFP9898363.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEESYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6576..7166
                     /gene="caiE"
                     /locus_tag="ACHOUK_002960"
     CDS             6576..7166
                     /gene="caiE"
                     /locus_tag="ACHOUK_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFP9898364.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYTDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7285..7680)
                     /gene="caiF"
                     /locus_tag="ACHOUK_002961"
     CDS             complement(7285..7680)
                     /gene="caiF"
                     /locus_tag="ACHOUK_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFP9898365.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(7941..11162)
                     /gene="carB"
                     /locus_tag="ACHOUK_002962"
     CDS             complement(7941..11162)
                     /gene="carB"
                     /locus_tag="ACHOUK_002962"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFP9898366.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11180..12328)
                     /gene="carA"
                     /locus_tag="ACHOUK_002963"
     CDS             complement(11180..12328)
                     /gene="carA"
                     /locus_tag="ACHOUK_002963"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFP9898367.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            complement(12784..13605)
                     /gene="dapB"
                     /locus_tag="ACHOUK_002964"
     CDS             complement(12784..13605)
                     /gene="dapB"
                     /locus_tag="ACHOUK_002964"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFP9898368.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKENGLFDMRDVLDLNNL"
     gene            complement(13772..14686)
                     /gene="rihC"
                     /locus_tag="ACHOUK_002965"
     CDS             complement(13772..14686)
                     /gene="rihC"
                     /locus_tag="ACHOUK_002965"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFP9898369.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLSTLPQLNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(14752..15702)
                     /gene="ispH"
                     /locus_tag="ACHOUK_002966"
                     /gene_synonym="lytB"
     CDS             complement(14752..15702)
                     /gene="ispH"
                     /locus_tag="ACHOUK_002966"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFP9898370.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(15704..16153)
                     /gene="fkpB"
                     /locus_tag="ACHOUK_002967"
     CDS             complement(15704..16153)
                     /gene="fkpB"
                     /locus_tag="ACHOUK_002967"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFP9898371.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(16241..16735)
                     /gene="lspA"
                     /locus_tag="ACHOUK_002968"
     CDS             complement(16241..16735)
                     /gene="lspA"
                     /locus_tag="ACHOUK_002968"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFP9898372.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVTLVVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(16735..19551)
                     /gene="ileS"
                     /locus_tag="ACHOUK_002969"
     CDS             complement(16735..19551)
                     /gene="ileS"
                     /locus_tag="ACHOUK_002969"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFP9898373.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPDEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFAHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYTEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(19594..20535)
                     /gene="ribF"
                     /locus_tag="ACHOUK_002970"
     CDS             complement(19594..20535)
                     /gene="ribF"
                     /locus_tag="ACHOUK_002970"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFP9898374.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(20543..20767)
                     /gene="yaaY"
                     /locus_tag="ACHOUK_002971"
     CDS             complement(20543..20767)
                     /gene="yaaY"
                     /locus_tag="ACHOUK_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFP9898375.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGSQFLPVICAMTMT
                     MKSHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            20870..21133
                     /gene="rpsT"
                     /locus_tag="ACHOUK_002972"
     CDS             20870..21133
                     /gene="rpsT"
                     /locus_tag="ACHOUK_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFP9898376.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT         5070 a         5751 c         5529 g         5047 t
ORIGIN      
        1 cagtggaatt aactcatgaa gaatgaaaag agaaaaacgg gaatagaacc gaaggttttc
       61 tttccgccat taataatcgt cggcatactt tgttggctta cagtcagaga tctggatgcg
      121 gcgaatgtcg ttattaatgc tgtattcagt tacgtcacca atgtatgggg atgggcattt
      181 gaatggtata tggtggtgat gcttttcggt tggttctggc tggtgtttgg cccgtatgcc
      241 aaaaagcgtt taggtaacga accacctgaa tttagtaccg ccagttggat ctttatgatg
      301 ttcgcctcct gtacgtctgc tgccgtactg ttctggggat cgattgagat ctactactac
      361 atctccaccc cgccgtttgg cttagaaccg aactcgacag gagcgaaaga gttggggctg
      421 gcttacagct tgttcctctg gggacctctg ccgtgggcca cttacagctt cctttcagtc
      481 gccttcgctt acttcttctt tgtccgcaaa atggaagtga ttcgccccag ctccaccctg
      541 gtgccgctgg taggtgaaaa acacgccaaa gggttgttcg gcactatcgt cgacaacttc
      601 tatctcgtcg ccttgatctt cgcgatgggt accagtctgg gccttgccac gccgctggtg
      661 accgagtgta tgcaatggtt gtttggcatt ccgcataccc tgcaactgga cgctatcatc
      721 attacctgct ggattatcct caacgccatt tgcgtcgcct gcggtctgca aaaaggggta
      781 cgtatcgcca gtgacgtgcg tagttacctg agcttcctga tgctgggttg ggtgttcatc
      841 gtcagcggtg ccagcttcat catgaactac ttcaccgatt cggtggggat gttgctgatg
      901 tatctgccgc gcatgttgtt ctataccgat cccatcgcta aaggcggctt cccgcagggc
      961 tggaccgtgt tctactgggc atggtgggtg atttacgcca tccagatgag tatcttcctc
     1021 gcccgcatct cccgtggtcg tactgtgcgt gaactgtgct tcggcatggt gatgggactg
     1081 acagcatcca cctggatcct gtggactgta ctcggtagta acactctgct gttgatagat
     1141 aaaaacatca tcaacattcc aaatctgatc gaacagtacg gtgtggcgcg cgccatcatc
     1201 gaaacctggg ccgctctgcc gctcagcacc gccaccatgt ggggcttctt catcctctgc
     1261 tttattgcca ccgttacgct ggttaacgcc tgctcttata ccctggcgat gtccacttgc
     1321 cgcgaagtac gcgatggtga agaaccaccg ctgctggtgc gtatcggttg gtcaattctg
     1381 gttggcatta tcggtattgt tttgctggcg ctcggcggcc tgaaaccgat tcaaactgcc
     1441 attatcgccg gaggatgccc gctgttcttc gtcaacatta tggtgacgct ctcctttatt
     1501 aaagacgcga aacagaactg gaaagattaa ttaaccccca aaatatcaag aggttgaaag
     1561 atggatttta atttaaatga tgagcaggaa ctgtttgtcg ccggtatccg cgaactgatg
     1621 gccagcgaaa actgggaggc ctattttgcc gagtgcgacc gtgacagcgt ctacccggaa
     1681 cgttttgtca aagcactggc ggatatgggt atcgacagtc tgctgatccc tgaagagcac
     1741 ggtggtctgg acgcggggtt tgttactctc gccgccgtgt ggatggagct gggacgtctg
     1801 ggggcaccaa cctatgtact gtaccagttg ccgggcgggt tcaatacctt cctgcgcgaa
     1861 ggcacacaag agcagatcga caagattatg gctttccgcg gcaccggtaa gcagatgtgg
     1921 aactcagcga ttaccgaacc gggcgcgggc tccgacgtgg gtagcctgaa aacgacttat
     1981 acccgtagaa atggtaagat ttatcttaat ggtagtaagt gttttattac cagtagcgcc
     2041 tacaccccgt acatcgtggt gatggcgcgc gacggggctt ctccggacaa acctgtctac
     2101 accgaatggt ttgttgatat gagcaaaccg ggcatcaaag tgaccaaact tgagaagctc
     2161 ggtctgcgta tggatagctg ctgtgaaatc acctttgacg atgtggaact ggacgagaaa
     2221 gacatgttcg gtcgggaagg taacggcttt aaccgcgtca aagaagagtt cgaccatgaa
     2281 cgtttcctgg tagccctgac caactacggt acggcgatgt gcgcctttga agatgcggcg
     2341 cgctacgcca accagcgcgt gcagtttggc gaggctattg gtcgtttcca gttgattcag
     2401 gaaaaattcg cccacatggc gatcaaatta aactccatga aaaacatgct gtatgaagca
     2461 gcgtggaaag cagacaacgg caccatcacc tctggcgatg cagcgatgtg caaatacttc
     2521 tgcgccaatg cggcatttga agtggtggat agcgcaatgc aggtgctggg cggtgtcggg
     2581 attgcgggca accaccgcat cagccgcttc tggcgtgacc tgcgtgtaga ccgcgtttcc
     2641 ggaggatctg acgaaatgca gatcctgacg ctgggtcgtg cggtgctgaa gcaataccgc
     2701 taagtccgtg cgctgcctga tgcgacgctg acgcgtctta tcaggcctac gggagcacga
     2761 atgtaggccg gataaggcgt ttacgccgca tccggcagtc atgcgctaca taaatttttc
     2821 aggagctaat aatggatcat ctacccatgc cgaaattcgg gccgttggcc ggattgcgcg
     2881 ttgtcttctc cggtatcgaa attgccgggc cgttcgccgg gcaaatgttc gcagaatggg
     2941 gtgcggaagt tatctggatc gagaacgtcg cctgggccga caccattcgc gttcaaccga
     3001 actacccgca actctcccgc cgcaatttgc acgcgctgtc gttaaatatt ttcaaagatg
     3061 aaggccgcga agcgtttctg aaattaatgg aaaccaccga tatcttcatc gaagccagta
     3121 aaggtccggc ctttgcccgt cgtggcatta ccgatgaagt actgtggcaa cacaacccga
     3181 aactggttat cgctcacctg tccggttttg gtcagtacgg caccgaggag tacaccaatc
     3241 ttccggccta taacaccatc gcccaggcct tcagtggtta cctgattcag aacggtgatg
     3301 ttgaccagcc aatgcctgcc ttcccgtata ccgccgatta cttttctggc ctgaccgcca
     3361 ccacggcggc gctggcagca ctgcataaag cgcgtgaaac cggtaaaggc gaaagtatcg
     3421 acatcgccat gtatgaagtg atgctgcgta tgggccagta cttcatgatg gattacttca
     3481 acggcggcga aatgtgcccg cgcatgagca aaggtaaaga tccctactat gccggttgcg
     3541 gtctgtataa atgtgccgac ggctacatcg tgatggaact ggtgggcatt acccaaattg
     3601 aagagtgctt taaagatatt ggcctcgcac atctactcgg tacgccggaa atcccggaag
     3661 gcactcagct tatccaccgt atcgaatgcc cttacggccc actggtagaa gaaaaactcg
     3721 atgcctggct ggcggcacat accatcgcag aagttaaaga acgctttgcc gaactgaata
     3781 tcgcctgtgc caaagtactg accgtaccgg aactggaaag caatccacag tatgttgccc
     3841 gtgaatcaat cactcagtgg caaacgatgg atggtcgcac ctgcaaaggg ccgaacatca
     3901 tgccgaaatt caaaaataac cccggacaaa tctggcgcgg aatgccctca catggcatgg
     3961 acacggctgc cattttgaaa aatatcggct acagcgaaaa cgacattcag gagttggtca
     4021 gcaaaggtct ggccaaagtt gaggactaaa cgcgtggcgt cagtcggctt gcctgactag
     4081 cgcccgccca ataacaaacg aacaaaaatg gatagaggtg caatggatat cattggcgga
     4141 caacatctac gtcaaatgtg ggacgatctg gcggacgttt acggtcataa aacggcgctg
     4201 atttgtgaat ccagcggcgg agtcgttaac cggtatagtt atcttgagtt aaatcaggag
     4261 attaaccgca cggcaaacct gttttatacg ctggggattc gcaaaggcga caaggttgca
     4321 ctacatctcg acaactgccc ggaatttatc ttttgctggt tcgggctggc aaaaattggc
     4381 gcgattatgg tgccgattaa cgcccgcctg ttacgcgagg aaagtgcgtg gatcctgcaa
     4441 aatagccagg cgtgcctgct ggtgaccagt gcgcaattct atcctatgta tcaacagatt
     4501 cagcaagaag atgccactca attgcggcac atttgcctga cagatgtggc acttcccgct
     4561 gatgatggcg tgagttcgtt tactcaactg aaaaatcaac aacctgccac cttgtgctat
     4621 gcaccgccgc tatcgactga cgatacggcg gaaattctct tcacctccgg caccacctcc
     4681 cgaccgaaag gtgtggtgat tacccattac aacctgcgct tcgctggata ttactccgcc
     4741 tggcagtgtg cactgcgtga cgatgacgtc tacctgacgg taatgcctgc gtttcatatc
     4801 gattgccagt gtactgcggc gatggcggcg ttttctgccg gggccacctt tgtgctggtc
     4861 gagaaataca gcgcccgcgc cttctgggga caggtacaga agtaccgcgc caccattacc
     4921 gaatgtattc cgatgatgat tcgtacgttg atggtgcagc cgccttcagc gaacgatcgg
     4981 caacaccgcc tgcgggaagt gatgttttat ctcaacttgt cggagcagga aaaagacaca
     5041 ttttgtgaac gcttcggtgt tcgcttgctg acgtcttatg ggatgacgga aaccattgtg
     5101 ggcattatcg gcgatcgccc tggcgataaa cgacgctggc cgtcgattgg tcgggcgggg
     5161 ttttgctacg acgcggagat ccgcgacgat cacaatcgcc cgctcccggc aggtgagatc
     5221 ggtgaaatct gtattaaagg cgtaccaggg aaaaccatct tcaaagagta ttttctcaac
     5281 ccgaaagcca ctgcaaaagt gctggaagct gatggctggc tgcataccgg cgataccgga
     5341 taccgcgacg aagaaggctt tttttatttc atcgatcgcc gctgcaatat gatcaaacgc
     5401 ggtggcgaga atgtctcctg cgtggagctg gaaaatatca tcgccaccca tccaaaaatt
     5461 caggatatcg tggttgtggg tattaaagat tcgattcgcg atgaagccat caaagcattt
     5521 gtggtgctga atgaaggtga aacattgagc gaagaggaat ttttccgctt ctgcgaacaa
     5581 aatatggcga aatttaaagt gccctcttat ctggagatca gaaaagatct gccacgtaat
     5641 tgctcgggga aaataattag aaagaatctg aaataaatga aacggcaggg aactaccctg
     5701 ccagctaata atcacgctct aaaacaatat gcattttttg cagggatgtt gtcgtccctg
     5761 aaaaagcaaa aatggagaaa aggaatgagt gaatcattac atctgacccg caatggatca
     5821 attctggaaa ttactcttga tcgcccaaaa gcgaatgcta ttgatgcaaa aaccagcttt
     5881 gaaatgggcg aagtatttct aaatttccgt gacgatccgc aattacgtgt cgccattatt
     5941 accggtgccg gagagaaatt cttttccgcg ggctgggatt taaaagcggc agcagaaggc
     6001 gaagcaccgg atgctgactt tggcccgggt ggttttgcgg gattaaccga aattttcaat
     6061 ctcgacaaac cggttatcgc agctgtgaac ggctacgcct ttggcggcgg tttcgaactg
     6121 gcgctggcgg cagattttat tgtttgtgcc gataacgcca gcttcgccct gccggaagcc
     6181 aaactgggca tcgttcctga cagcggcggt gtgctgcgtc tgccgaagat cctgccgcct
     6241 gccatcgtca atgaaatggt gatgaccggc agacgaatgg gcgcagaaga ggcgctgcgt
     6301 tgggggatag tcaaccgcgt ggttagccag gcggaactga tggataacgc ccgcgaactg
     6361 gctcagcagc tggttaacag cgccccgctg gcgattgcgg cgctgaaaga gatctaccgc
     6421 accaccagcg aaatgccggt agaagaatcg tatcgctata ttcgcagcgg cgtgttgaaa
     6481 cactatccat cggttctgca ttcggaagat gccattgaag ggccgctggc gtttgccgag
     6541 aagcgcgatc cggtgtggaa aggacgttaa caaccgtgag ctattacgcc tttgaggggt
     6601 taattccggt ggttcacccg acggcgtttg tccatcccag tgccgtcttg attggcgatg
     6661 tgattgtggg agccggtgtc tacatcggcc cactcgcctc actgcgtggt gactacgggc
     6721 ggttgatcgt gcaagcggga gccaatattc aggatggctg cattatgcat ggctacaccg
     6781 acactgacac catcgttggg gaaaacggcc atatcgggca cggagcgatc ctgcatggtt
     6841 gtgtgattgg tcgcgatgca ttggtcggga tgaacagcgt gattatggat ggcgcggtca
     6901 ttggcgaaga gagcattgtt gccgccatga gctttgtcaa agcaggcttt cacggcgaga
     6961 aacgccagtt gttgatgggt acgccagccc gcgctgtacg cagtgttagt gacgacgagt
     7021 tacactggaa acggttgaat accaaagagt atcaggatct tgtcgggcgc tgtcatgcat
     7081 cgttacatga aacacagcca ctgaggcaaa tggaggaaaa tcgcccccgt ttgcagggga
     7141 cgacggatgt gacgcctaaa cggtgatctt gccggatgcg gcgtaaacgc cttatccaac
     7201 tctgctggag agcaaccgta ggccggataa gatgcgccag catcgcatcc ggcgaccagg
     7261 tcgcacggta tcatcgaaat gacattaacg acgcatactc tttgacaaca tcatctgcca
     7321 cttctgctcg cggttaagtt ccgtgagcgg aacggcagga atacgcggtg tctttcttac
     7381 gccgactaat ttttcccgac tgttattgcg caggcgcgcg taaatttgct catcgatatc
     7441 gaccacttta accagtcgct gacactggca tccccgccct tccagcttat tagggatcat
     7501 cttaacttcg cagcttattt ctgtgacttc cgacagaata taagtcaggg tattaaccgc
     7561 cttgctgtgt tcaatatcga aatgaataga gatctctctt gctatcaccc accgattttc
     7621 agccatcatc cattcggcga ttaacaagta gagtggtttt tcaacatatc cttcacacat
     7681 acgaactcca gtttcacaaa ttaaaagtct ggctatgact cgttttgaag caattaaaca
     7741 aggccatatt aaaattgtgg atatatcgat agtatgcaac ttcaccgtcg ataagtgtga
     7801 gctaaatcct gcttatgtca tcaaaaatat cgattcccca aaagatttac ttaatgggca
     7861 aaaaaaacgg tcctcatcag agaaccgtat tagttattcg atcaaattag cggatgaaaa
     7921 atatccgcca tgacacgcta ttatttgatc tgtgcgtgca tttcctgcac cgaaattact
     7981 ttttcagtcg catcggcatt cagcgccatc gcggtagcga aaccaccgtt cagggtggtg
     8041 tcataatgca ctttatattg cagcgcactg cgacggatca cgcgggagtc ttcaatcgca
     8101 cgacggcctg aggtggtgtt gatgatgtag gtatattcgc cattcttgat acggtcctga
     8161 atgtgcggac ggccttcatg caccttgttt accagacgcg gattgatacc cgcttcgccc
     8221 agcacaatcg ccgtgccgtg ggtcgcatcc agctcgaagc cctgtttcag cagttttgcc
     8281 gccaggtcca ccacgcgttc tttatcgcct tcgcgcacgg aaagcagcgc acgaccgtgt
     8341 ttcttcatgg tggagttgct gcccagctgc gctttggcaa acgcttcagc gaaggtgcgg
     8401 cccacgccca tgacttcccc ggtagaacgc atttctggcc ctaacagcgg gtcaacgccc
     8461 gggaatttat tgaacggcag caccacttct ttcaccgagt agtacggcgg gataacttct
     8521 ttggttacgc cctgctcagc cagcgatttg ccagccatca cacgcgccgc cacttttgcc
     8581 agcggtacgc cggtggcttt ggagacgaac ggaacggtac gcgccgcacg cgggttaact
     8641 tcaatcaggt agacttcgtt gtttttcacc gcaaactgca cgttcatcag gccgcgcacc
     8701 tgcaattcga aggccagttt ctgcacctgc tggcgcatta catcctgaat ttcctgactt
     8761 aaggtgtacg ctggcagcga acatgcggag tcaccggagt gcacgcccgc ctgctcgatg
     8821 tgctccatga tgccgccaat cagcaccatt tcgccgtcgc agatggcatc cacgtcaact
     8881 tctactgcgt catcaaggaa atgatccagc aacactggcg catcgttaga cacgctaacc
     8941 gccgtctgga agtagcgacg caggtcagct tcgtcataga cgatttccat cgcccgaccg
     9001 ccgagaacgt aagacggacg caccaccagc gggtagccaa tctcttttgc cttctcaact
     9061 gccatttcaa tagcagtaac ggtggcgttc gccggttgtt tcagtttcag acggtcaacc
     9121 gcatgctgga agcgttcacg gtcttccgca cggtcgatag catccgggct ggtgccgata
     9181 accggtacgc cagcggcttc cagtgcacgc gccagtttca gcggggtctg accgccgtac
     9241 tgaacgataa cgcctttcgg cttctcaata cgcacgattt ccagcacatc ttccagagtt
     9301 accggctcga agtagaggcg atcggaagtg tcgtagtcgg tagagacggt ttccgggtta
     9361 cagttaacca taatggtttc gtaaccgtct tcacgcagcg ccagcgaggc gtgtacgcag
     9421 cagtagtcga attcgatacc ctgaccgata cggtttggac cgccgccaag caccatgatt
     9481 ttttcacggt cggtagacgg attcgcttcg cactcttctt cataagtgga gtacatgtaa
     9541 gcggtgtcgg tggcaaactc tgccgcacag gtatccacgc gcttgtagac cgggtgcagg
     9601 tcatattggt cacgcagctt acggatttcc gcttcgcgta cgcccgccag ttttgccaag
     9661 cgcgcatcgg caaagccttt gcgtttcagc tggcgcagga attcagcgtt caggccagtg
     9721 atgcccactt ccgccacttt ctcttccaga cgcaccagtt cttcaatctg taccaggaac
     9781 cagcggtcaa tgttagtcag gttgaagacg ccgtccacag acaggcccgc gcggaaagca
     9841 tcggcgatgt accagatacg ctctgcgcct gcgtctttca gttcgcgacg gattttggtt
     9901 aacgcttccg ggtcatccag gctcactttc gggtcgaatc cagtcgcacc gacttccagg
     9961 ccgcgcagcg ctttttgcag ggattcctgc tgcgtgcgac caatcgccat cacttcgcca
    10021 accgatttca tctgagtggt cagacggtcg ttagcaccgg cgaatttttc gaagttgaag
    10081 cgaggaattt tggtaaccac gtaatcgatg gacggctcga aggaggccgg agtacgtccg
    10141 ccagtgatgt cgttcatcag ttcatcgagg gtgtaaccca ccgccagttt cgccgccact
    10201 ttagcaatcg ggaaaccggt cgctttcgac gccagcgccg aagaacggga cacgcgtggg
    10261 ttcatttcga taacaatcag gcgaccgttt ttcgggttca ccgcaaactg gacattggaa
    10321 ccaccggttt caacgccgat ttcacgcagc accgccatcg aggcgttacg catgatttga
    10381 tattctttgt cggtcagcgt ttgggctggc gcgacagtga tggagtcacc ggtgtggatg
    10441 cccatcgcat caaagttttc gatagagcag acgatgatgc agttgtcgtt tttatcgcgc
    10501 accacttcca tctcgtactc tttccagccg atcagcgact catcaatcag caactctttg
    10561 gtcggagaga gatccagacc gcgggcgcaa atttcttcaa actcttcgcg gttataagcg
    10621 ataccgccgc cgctaccgcc catggtaaag gatgggcgaa taatgcacgg gaagcccacg
    10681 tcagcggcaa ccgccagcgc ttcttccatc gtatgtgcga taccggaacg cgcggtttcc
    10741 agaccaattt tcttcatcgc tacgtcgaaa cgacggcggt cttctgcttt atcaatcgca
    10801 tcggcagtgg caccaatcat ggtgacgccg aactcttcca acacgccctg acgttccagc
    10861 tccagcgcgc agttcagcgc cgtctgaccg cccatggttg gcagcaccgc gtccgggcgc
    10921 tctttttcaa taatcttacg taccacttcc cagtgaatcg gctcgatgta ggtcgcatcg
    10981 gccatttccg ggtcggtcat gatggtcgcc gggttggagt tcaccagaat gacgcggtaa
    11041 ccctcttcgc gcagggcttt acatgcttgc gcgccagagt agtcaaactc acacgcctga
    11101 ccgataacaa tcgggcccgc acccagaatc aggatacttt ttatatctgt acgttttggc
    11161 atggctcttt tactcctgat tacttagcgg ttttacggta ctgctcaatt aactcgataa
    11221 agtggtcgaa caacggcgcg gcgtcgtgtg gaccagggct ggcttcaggg tgaccctgga
    11281 agctgaacgc cggtttatcg gtgcgatgaa tgccctgtaa cgtaccgtcg aacagggatt
    11341 tatgcgtgac acgcaggttt gcaggtaatg ttgcttcatc caccgcaaaa ccgtggttct
    11401 gggcggtgat catcaccacg tttttctcca catctttaac cggatggttg ccgccgtggt
    11461 gaccaaattt cattttgaca gtcttcgcac cgctcgccag cgccagcagc tgatgaccga
    11521 ggcagatgcc gaagaccgga atatcggttt cgaggaattt ctggatggcg gtaatggcgt
    11581 aatcgcacgg ggccgggtcg ccaggaccgt tggagaggaa gatgccgtct ggattcattt
    11641 tcagcacatc ttccgcagaa gtttgcgccg gaacgatggt caggcgacag cctctatcca
    11701 ccagcatccg caggatgttg cgtttggcac caaaatcata agccacgacg tggaacggca
    11761 gctcgtcttc ttttttcgct tctggcaggc cgccggtcaa cgtccagctc ccttgtgtcc
    11821 agctataggc ttctgcggtg gtcacttctt ttgccagatc catgccattc agacctggga
    11881 acgcgcgggc tttttctaac gccagcgccg catccgggtt atcgcctgcg ataatgcagc
    11941 cattctgtgc gcctttctcg cgcagtaaac gcgtcagctt acgggtatcg atatcggcaa
    12001 tcgccacgat gttatggcgc ttcaggtaag aagagaggtc ttcggtatta cggaagttgc
    12061 tggcaatcag cggcaggtcg cgaatcacca gaccttgtgc atgtacctga gaagattctt
    12121 catcggcgtc attagtgccg acattgccaa tatggggata agtaagagta acgatttgac
    12181 gagaatagga aggatcagtg aggatttctt gataaccggt cattgaagta ttgaaaacga
    12241 cttccccaac cgccgaacct gttgccccta tggcccgacc gtgaaactgg gttccgtctt
    12301 ccagaaccaa tagcgctgac ttaatcaaaa caccctccag agaatattca ctcactttat
    12361 ttgcatatta attcacaatg atgacgtaaa tcaatgcaaa tctgcttacc gacgaatttc
    12421 tggcaaacgg cggcattctg gagatatggg cgctaaaagt caacttaatg gcaggatttt
    12481 ttatattatt ttgtggctca tgcatgtttt acaggcatta agcgacaaaa agatcatcta
    12541 acctgttctg gaaaacgctt gcgcagcaag aagattgcat gaaataaaaa cgagcaggca
    12601 aaagtggacc aaatggtcaa aatttacatt aagacaacaa aaatagaaga caaaacatgt
    12661 aatttttggc agttatagaa aaattaaaag cataacacca aaaataaaag ggctaaaaat
    12721 taaagccctt tataattaat aaattaaata tgttactttt gcatcatcat aaacattttg
    12781 tggttacaaa ttattgagat caagcacatc tcgcatatca aaaagaccat tttccttacc
    12841 actcaaccac aaagccgatc ttaccgcacc gttagcaaat gtcatgcggc tggacgcctt
    12901 atgggtgatc tccagacgct cgccaatatc ggcaaacatc gcggtatgtt cgccaacgat
    12961 gtcacctgca cgcacggtgg caaaaccaat ggtaccaggc acacgttcac cggtatggcc
    13021 ttcacgactg tagaccgcgc aatctttcag atctttatca agggcgtggg cgatcgcctc
    13081 tcccattgcc agtgcggtgc ctgacggcgc atcaacttta tgtctatgat gtgcttcaat
    13141 aatttcaata tcggtgtagt cacccatcac tttggctgct ttctccagca gcttaagcat
    13201 gacgttaacg ccaacgctaa aattggcggc aaagacaatc gcaatatcgg cggcggcgtc
    13261 acgaattgcc tgttttccgg cttcgtcaaa ccccgtagtg ccgatcacca tccctttgcc
    13321 atgctggcga caaaaagcga gatggttcag cgtaccttcc ggacgggtaa aatcgataaa
    13381 cacatcaaaa tcatctttta tcgcatcaag actgctttgc acggtaacgc ctgttttccc
    13441 tgcaccagcc agctcaccgg cgtcgctgcc cagtaaagaa gatccttcgc gctccagcgc
    13501 cgcgcccagc tgcacgcctt ctaatgccag tgccgcctga atcaactggc ggcccatacg
    13561 ccccccggct cccgcgatgg caacgcggat gtttgcatca tgcatagcta ttctcttttg
    13621 ttaatttgca tagaccgttt tcagagtaac cagcgcatcg ctacgccgcc agccaaaaac
    13681 gctgataatt agaaattaat gataaacagg gagggatatc agtgaaaggc atgagtcgat
    13741 gaatgactgc atgccgataa catgtgacag gttacggcac cagagccagc acctcagcca
    13801 cccactgttg gaagcctttt acattcagat ccaatgccac ctgtacattg gctggtttgc
    13861 ccaggcaacc gtcgatatca accaccgtcg tgcctgaggt aaattcgcct tgagtttcca
    13921 ctgccacaaa acagggtttg agagtgaaca ggtccgggcg caccagccag gcgatggcgc
    13981 agagatcgtg cattcgcaag ccgctttgca tactgccgct acggtagtgg ctaaacaggg
    14041 cgtgaagcat tttcccggta cggtttaact gcggcagtgt agagagatag tcaggagtta
    14101 atattgcctg attggtgaca tccaaaccgc acatgacgat ttcaatacca ctgcggaaga
    14161 cacaggcagc agcttctgga tcggcagcaa tattaaactc ggcgtttggc gtacagttgc
    14221 cgcgtccggc agaaccaccc atgatcacca gacggcgaat atacggttta cattccgggc
    14281 attgtgaaag taacagcgca atattggtta acgggccgat ggccaccagg gtaacgggct
    14341 ctggtgcacg catcagggca tcccgaatcg ccagaaacgc cggtatcccg agtggctttc
    14401 ggttgtgctc aacaaagtcg tagccagcca ttcccgattc gccgtgcaca gatgctgcat
    14461 cacgcggtgc gcgtaccagc ggcacagcgg ctccctgggc gagcggaatc tccgcattcc
    14521 agaaatgcag cagttgcaag gcgttacggg tagttttctc aaccgagaca ttacccgcga
    14581 cggtggtcat cagttgcagg tcgagttcgg gggcaaaaat cgcggcggca atggcgacgg
    14641 catcgtcaat gccggggtca gtatcgagga agataggtaa gcgcatgttt tctccataaa
    14701 aaatgccgat aacaagaccg gcattttcgc ataacttagg ctgctaatga cttaatcgac
    14761 ttcacgaata tcgacacgca gctctttcgg cacttcgaaa acaatatttt cttcacggcc
    14821 ttccagcgga atggcttcac caccacccag ctgctgcaaa cgtgccacca cattctgcac
    14881 cagaatatcc ggagccgatg cgcccgcagt cacgccgacg catttaacct ctttcaccca
    14941 ctcttcctgg atatctttcg catcgtcaat caaaaacgcg cgtttgccca tacgctgggc
    15001 cagctccgcc agacggttgg agttggagga gtttttcgaa ccgaccacca acacaacttc
    15061 cgcctgttct gccagggcgc gtaccgcttc ctgacggtta gtcgtggcgt agcagatgtc
    15121 atctttgcgc ggaccgacaa ttttcgggaa gcgtttacgc agcgcgtcga tcacatcaga
    15181 cgtgtcatct accgacagcg tggtttgggt cataaaggag agcttctctt cgtttttgac
    15241 cgtcagtttc cacacatcgt caggcgattc gaccagatac attccccctt cagggttgct
    15301 gtactgcccc atcgtccctt ccacttccgg gtgcccggcg tgaccgatga gaatagactc
    15361 ttcgccacga cggctggcgc gggcgacttc catatgcact ttggtcacca gcggacaggt
    15421 ggcgtcgaat accgtcaaat cacggctttt cgcttcgtta cgtaccgcct gagaaacacc
    15481 atgtgcggag aagatcagga tcgcgccgtc cggcacttcg ctgatctgct caataaagat
    15541 agctccacgc tcgcgcaggc tatcgaccac gtagcggtta tgcaccactt cgtgacggac
    15601 atatatcggt gcgccgtaaa tggcaagcgc gttttcaaca atgctgatag cgcggtctac
    15661 cccggcacaa aaaccacgtg ggttggccaa caggatctgc atgttacgcc tccagtgccg
    15721 gatcgatttc cagcacttca atatcaaaat gaacggtctg cccggccagc ggatggttga
    15781 aatcgacggt aatggagtcg ccgttaattt cgcggatcac gccaggcatc tcactgccat
    15841 ccattgcggt aaaaagcatt attgcgccaa tttctggctc gcctgcatcc ataaactcac
    15901 ggcgggagaa gtactgaatc aggtccggtg acggcacgcc aaacgccgca tctggctcca
    15961 gcgagaaagt ggttttatcg cccactttca gccccagcag gtgttgctcc agcccttcgg
    16021 aaagagaagc atcgcccagg cggaacagcg ccggtttacc gttgttgcgg gtagactcgg
    16081 cggtggtgcc atcgtcgagt tttagcgtga agtgcaccag gacggcgcta ttgctctgta
    16141 cagattcaga catgcaggtt tgctcttata ttttgaggat ttttgccgga tgcggcctac
    16201 gatttcgcag caatctgtag gccttatccg gcagggttta ttattgtttt ttcgctttag
    16261 aaggcaaaaa accttccagc acaatcagtg ccgcaccaac acagatggca gtatcggcga
    16321 ggttgaaggt ggcgaagtgc cagtcgccga cgtagaagtc gatcatatcg acaacgaagc
    16381 cgtgccacag gcggtcgaac aggttgccca gcgcgccgcc aataatcagc gcgtaggcga
    16441 tattgtttag cttctgcgtg gccttcgagc gatacatcat cacaaccagg gtaacgctaa
    16501 taccaatcgc aataccggca aagaaccaac gctgccagcc gccgctatcg gcaaggaaac
    16561 taaacgccgc gccatagtta cgcgcataat gcagattaag cgacgggaac agcgggaccg
    16621 tatcccccag agcaaagttc tggaggatca ggtatttgct gcccagatcg ataatcagca
    16681 cgactaccac cagccacagc cagcgtagcc ctgttgaaca gatcgattga ctcatcaggc
    16741 aaacttacgt ttttcaccgt caccggcgac gttgctgaca cagcggccgc agatttctgc
    16801 gtgttccgcc accttgccga catcctgggt gtagtgccag cagcgtgggc acttctcacc
    16861 ttcggcttta ctcaacgcga ctttcagccc tttgagcact tcgctctgct gagcatcagc
    16921 aggtgcgtcg ttatagtctg caacggtagc gccggaggtc aacaggacaa atcgtaattc
    16981 atcgcccaat gcggtcagtt tcgccgccag ttccggttct gtatacaagg ttactgccgc
    17041 ttccagcgag ccacccactt tcttgtcggc acgcgcctgc tcaatgactt tgttcacttc
    17101 gccacgcact ttcaacagct cgtcccagaa cgcatcgttc attgcttcac tgtctgccag
    17161 accaaacaga ccttcgtacc actcgccggt gaagacgtat ttttcacgtt cgcccggcag
    17221 gtagccccac acttcatcag cggtgaagga gaggattggt gccatccagc gaaccagcgc
    17281 ttctgcgatg tgatacagcg cagtctggca gctacgacgc gccacgctgt ccgctttggc
    17341 ggtgtactga cggtctttga tgatgtcgag gtagaaggaa cccatctcaa cggagcagaa
    17401 gcgcatcaga cgctgtacca cttcgtggaa atcgtatgct tcgtatgcct tgaggatgtc
    17461 ttcctgtgcc gctttcgcac aacctacggc ccagcgatcc agtactacca tctcttccgg
    17521 tttcaccata tcttttgccg gatcaaaacc gttcaggttt gccagcagga agcgcgcggt
    17581 gttacggata cgacgatagc tgtcggcagc acgtttcagg atctcgtcag aaacggccat
    17641 ttcaccggtg tagtcggttg atgccaccca cagacgcaga atatccgcgc ccagtttgtt
    17701 catcacatcc tgcggcgaca cggtgttgcc gatggattta gacatcttgc ggccctgacc
    17761 atctacggta aagccgtggg tcagcacctg acgatacggc gctttgccct tcatcgcggt
    17821 ggagatcatc agagaagaca tgaaccagcc acggtgttgg tcagaacctt ccagatacat
    17881 gtccgcagcg tgaccggcaa attccgggcg cacgtcaaca acagaagagt gagtagagcc
    17941 ggagtcaaac catacatcca gcgtatccgg cactttcaca tactgatcag cttcatcgcc
    18001 gaggatctct ttcgcatcaa gatcccacca cgcctggatg ccatcaactt caacgcgttt
    18061 agccacttct tccatcagtt cgagggtacg cggatgcagc tcttccgtgt ctttgtgcac
    18121 gaacagagac atcggtacgc cccaggtacg ctgacgggag atacaccagt caggacggtt
    18181 agcgaccatc gactcgatac gcgcctggcc ccagtccggg atccactgca cacctttgat
    18241 ctctttcaga gactgcgcgc gcagaccttt ctgatccata ctgacgaacc actgcggcgt
    18301 tgcgcggaag atgatcggcg ttttgtgacg ccagcagcac ggatagctgt gctgcatttt
    18361 ctcaacgtgc agcagcgcgc ctttttcctg cagcagcgca acgacgatgt cgttcgcttt
    18421 gaagacgttc acgccatcca gcgtcggata agtgcccggc agataagtgc cgtccgggcc
    18481 aaccgggtta gcggtttcca ggccgtattt ctgaccgatc acatagtcgt ccgggccgtg
    18541 gccaggcgcg gtgtgaacgg caccggtacc ggcatccagg gtaacgtgat cgccgaggat
    18601 tgccgggacg tcgaagccca taaacggatg ggcaaagcgc agcagctcaa gctccgcacc
    18661 ttttaccgtg ccgagaatgg tgtaatcggt cacgccgata cgctgcatta cgctttcaac
    18721 cagatctttc gccagaatca cggcctgacc gtcgatctgc accagcgcat agtcgaaatc
    18781 aggtgcaata gagattgcgc ggttcgcagg cagagtccac ggcgtagtgg tccagatcac
    18841 cagcgagatt gggccgttaa cgttgcttac gccaaatttc gctttcagcg catcctgatc
    18901 gaccgcctgg aaagcgacgt caatggacgg agaagttttg tcgtaatact caacttccgc
    18961 ttctgccagt gcagaacggc agtctacgca ccagtgcacc ggcttcgcgc ctttgtgcag
    19021 gtgaccattg ccgatgattt tgcccagcgc gcggatgata ttggcttcag ttttgaagtc
    19081 catggtcagg tacgggtgcg accagtcgcc cagcacgccc agacggataa agtctttgcg
    19141 ttgaccgtca acctgggtcg ccgcgtattc gcggcacttg gcgcggaact cggcggcggt
    19201 gaatttctca tccggcttac cgtattcttg ctctactttc agctcgatcg gcagaccgtg
    19261 gcagtcccag ccaggcacat acggcgagtc ataaccggaa agccctttgg acttcacgat
    19321 aatgtctttc agaatcttgt taaccgagtg accaatatga atgctgccat tcgcataagg
    19381 agggccatca tgcagaatga aggttttttt gcctttttta gccgcacgaa tgatgccgta
    19441 cagatcatca tcagtccaac gcgccagcat tcccggttcg cgcttggcga gatcgccacg
    19501 catcgggaac cctgtttccg gcaaattcag ggttgattta tagtcactca tcagattctc
    19561 ggttccgtgt ttcggtttga ttacataaca ggcttaagcc ggttttgtta gcccaaaaaa
    19621 ttcgcgggcg gttaattcat cacgcgcaat ctgcgctttc agttcgtcca gcgaagcaaa
    19681 tcgctgctca ttgcgtattt ttttacgcag cactacttgt atatggcgac cgtaaaggtc
    19741 cattgcaaca tctaacaaat gcacttccag ctgctggcga ataccggcaa ccgttgggcg
    19801 tgttccgatg tttgccacgc cgggtaacgg cttttcaccg aggcccagca cttctaccgc
    19861 ataaacccct ttcaccgggg aaacctgacg gcgtagcggt acattcgccg tcgggaaacc
    19921 tatagtgcgc cctaattcat caccgtggac tacacgcccg gagatggcaa acgggtgccc
    19981 cagtaaactt tctgccagag ccaaattgtc atccgcaagg gcctgacgca cggcggtgct
    20041 gctgatacgc acgccacctt cgcaaaaagt ttgcgtactg gtgatatcga agccgtattc
    20101 catgccagct ttctgtaata acaagaaatc gccttcccga ccagcgccaa agcggaaatc
    20161 atcccccacg gcgaggaatt ttacccgcaa atgcttcacc agaagatcgc tgatgaaatt
    20221 ttgcgcggtt aacgccgcga aacgcctgtc gaaacgtacg cacagcacgt aatcaacgcc
    20281 acactctgca aggtaacgta gtttttcccg cagtcgggtc agccgtgccg gggctttatc
    20341 ggtagcaaac agttccagtg gttgaggttc aaaaagcatc accatcaccg gtaagttgcg
    20401 cttgcgccct tcttcctgca agccctgtaa cagcgcgcga tgaccgcgat gcacgccgtc
    20461 gaaattacca atagtcagca cacacccttc ttgcggggcc tggctgagat tatgtatgcc
    20521 gcgtatcagc ttcatgtctg gctcaaaaca gtgaaaatcg tccgagtata ccttgtacag
    20581 cggtcaaggt taaccggcga ttgagtaccg aaatcagaaa gaagtggctt ttcattgtca
    20641 tggtcatggc gcaaatcacg ggaagaaact gactgcctgc tgcaattttt atcgcggaaa
    20701 agctgtattc acaccccgca agctggtaga atcctgcgcc atcactacgt aacgagtgcc
    20761 ggcacattaa cggcgcttat ttgcacaaat ccattgacaa aagaaggcta aaagggcata
    20821 ttcctcggcc tttgaattgt ccatatagaa cacatttggg agttggacct tggctaatat
    20881 caaatcagct aagaagcgcg ccattcagtc tgaaaaggct cgtaagcaca acgcaagccg
    20941 tcgctctatg atgcgtactt tcatcaagaa agtatacgca gctatcgaag ctggcgacaa
    21001 agctgctgca cagaaagcat ttaacgaaat gcaaccgatc gtggaccgtc aggctgctaa
    21061 aggtctgatc cacaaaaaca aagctgcacg tcataaggct aacctgactg cacagatcaa
    21121 caaactggct taattgccaa tttgctgaag ctttgtgaaa aagcccgcgc aagcgggttt
    21181 ttttatgcct gctgcttttg tgatgcgtga gagaacacac tgccggttgc cagatagtca
    21241 aaagagacat gccgttgcac tatctgaact cttcgtggct taagaatcgt agaaccatcg
    21301 gctgatacat tctgaacaag aatatctact caatctccag actcccctac aaagaatgtg
    21361 gatattgtga tacacattga ggtattaaac ttaagac
//
LOCUS       DAZKIP010000072        21347 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZKIP010000072 DAZKIP010000000
VERSION     DAZKIP010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.072"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            26..2056
                     /gene="malS"
                     /locus_tag="ACHOUK_002973"
     CDS             26..2056
                     /gene="malS"
                     /locus_tag="ACHOUK_002973"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFP9898377.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDASAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKAIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCIAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            2234..3487
                     /gene="avtA"
                     /locus_tag="ACHOUK_002974"
     CDS             2234..3487
                     /gene="avtA"
                     /locus_tag="ACHOUK_002974"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFP9898378.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEAQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(3639..4112)
                     /gene="ysaA"
                     /locus_tag="ACHOUK_002975"
     CDS             complement(3639..4112)
                     /gene="ysaA"
                     /locus_tag="ACHOUK_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFP9898379.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(4214..4354)
                     /locus_tag="ACHOUK_002976"
                     /pseudo
     CDS             complement(4214..4354)
                     /locus_tag="ACHOUK_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
     gene            complement(4356..5171)
                     /locus_tag="ACHOUK_002977"
     CDS             complement(4356..5171)
                     /locus_tag="ACHOUK_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9898380.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHSARRLESWEIIFVEKGRL
                     TIQEEERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDNDPRWLTHLS
                     VPQQTKVVDPQALVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADGAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFRKHTGLTPAAWKRRYSKEHINS"
     gene            5397..6797
                     /locus_tag="ACHOUK_002978"
     CDS             5397..6797
                     /locus_tag="ACHOUK_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9898381.1"
                     /translation="MTSTPITTADIAKGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCARINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVALFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSGPA
                     YQTLWLAVILGNGVILGFTLPLHFSLMAFADDYGEWKTHVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTASSLGGIAAMETLLPALFHLLLAGAIRFCKLNNP
                     MMSRIATDLRQRHVQP"
     gene            6808..8778
                     /locus_tag="ACHOUK_002979"
     CDS             6808..8778
                     /locus_tag="ACHOUK_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HFP9898382.1"
                     /translation="MNISEVDLHKLTVSDPFLGQYQQLVRDVVIPYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQEGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIASAQCEDGYLNTYFTVKAPEERWSNLAECHELYCAGHLIEAGVAFFQATGKRRLLG
                     VVCRLADHIDSVFGPDESKLHGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGA
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPLAQQQTAIGHAVRFVYLMTG
                     VAHLARLSHDDSKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFSSDYDLPNDTVYAE
                     SCASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKS
                     LKFNHIYDHVKPIRQRWFGCACCPPNIARVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIAVESPQPVRHTLALRLPDWCTQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEKADN
                     GESLHNLWLPTDAPFTTFEGKGLFSHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     TQTLTFIPWFSWANRGEGEMRIWVNEEKHCHP"
     gene            complement(8908..9648)
                     /gene="yiaT"
                     /locus_tag="ACHOUK_002980"
     CDS             complement(8908..9648)
                     /gene="yiaT"
                     /locus_tag="ACHOUK_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HFP9898383.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
     gene            9772..10746
                     /gene="yiaU"
                     /locus_tag="ACHOUK_002981"
     CDS             9772..10746
                     /gene="yiaU"
                     /locus_tag="ACHOUK_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9898384.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTLFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
     gene            complement(10743..11879)
                     /gene="yiaV"
                     /locus_tag="ACHOUK_002982"
     CDS             complement(10743..11879)
                     /gene="yiaV"
                     /locus_tag="ACHOUK_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFP9898385.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQKAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELNEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(11885..12208)
                     /gene="yiaW"
                     /locus_tag="ACHOUK_002983"
     CDS             complement(11885..12208)
                     /gene="yiaW"
                     /locus_tag="ACHOUK_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFP9898386.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQSHDSSVMQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            12391..12534
                     /locus_tag="ACHOUK_002984"
     CDS             12391..12534
                     /locus_tag="ACHOUK_002984"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9898387.1"
                     /translation="MNNFSKTSTMQIKRIKPSIIYRLLLIVLGIPMVIYGLVCPLTIE
                     TRD"
     gene            complement(12753..14291)
                     /gene="aldB"
                     /locus_tag="ACHOUK_002985"
     CDS             complement(12753..14291)
                     /gene="aldB"
                     /locus_tag="ACHOUK_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFP9898388.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKNRYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAEAWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(14399..15694)
                     /locus_tag="ACHOUK_002986"
     CDS             complement(14399..15694)
                     /locus_tag="ACHOUK_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFP9898389.1"
                     /translation="MAIKSPPTLIPLSHLSGEELQAHLRFNRVTDEKGRYLPFDELQY
                     RIKKGENVDVAWTLTRLARNAAIQRINYCNEAGEQAGFNITPVIAEACELVDKRATAL
                     ALKDQTERLRGAGAELSQLRLEEPITSSQLEGANTTTLVARKMLETGRSPRTEDEHMI
                     AGNARLMAEIPHLLAEPLTPALIRQLHAIGMGGINDAKYRPGEFRETDDVVIADYDGN
                     IVHQPPAAALLPERLEKVCQWLNSHEGYIHPLVRACILHFMLAHEHPFRDGNGRTSRA
                     LFYWYMLKSGYDVFKYISISRLLHAAPVKYAASYQYTESDGMDLTYFLEYQAGVIKRA
                     LQNWQQHIDEITQRSAKLDSVLFSSGVLKRLNPRQVTLLNVMLANPGKEYTVAEISAS
                     LGVSDNTVRADLRTIVKEGFAQEKKINDQQAVYFAHYPL"
     gene            complement(15824..16975)
                     /gene="yiaY"
                     /locus_tag="ACHOUK_002987"
     CDS             complement(15824..16975)
                     /gene="yiaY"
                     /locus_tag="ACHOUK_002987"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFP9898390.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLAANALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            complement(17164..19008)
                     /gene="selB"
                     /locus_tag="ACHOUK_002988"
     CDS             complement(17164..19008)
                     /gene="selB"
                     /locus_tag="ACHOUK_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFP9898391.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDPPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQCLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(19005..20396)
                     /gene="selA"
                     /locus_tag="ACHOUK_002989"
     CDS             complement(19005..20396)
                     /gene="selA"
                     /locus_tag="ACHOUK_002989"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFP9898392.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(20494..21102)
                     /gene="yibF"
                     /locus_tag="ACHOUK_002990"
     CDS             complement(20494..21102)
                     /gene="yibF"
                     /locus_tag="ACHOUK_002990"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFP9898393.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVTQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
BASE COUNT         5201 a         5732 c         5289 g         5125 t
ORIGIN      
        1 tcgcagcaat aaggactcat ccaccatgaa actcgccgcc tgttttctga cactccttcc
       61 tggcttcgcc gttgccgcca gctggacttc tccggggttc cctgccttta gcgaacaggg
      121 aacgggaaca tttgtcagcc acgcgcagtt gcccaaaggt acgcgtccac tcacgctaaa
      181 ttttgaccag cagtgctggc agcctgcaga tgcgataaaa ctcaatcaga tgctttccct
      241 gcaaccttgt agcaacacgc cgcctcaatg gcgattgttc agggacggca aatatacgct
      301 gcaaatagac acccgctccg gtacgccaac attgatgatt tccatccaga acgccgccga
      361 accggtagca aacctggtcc gtgaatgccc gaaatgggat ggattaccgc tcacgctgga
      421 tgtcagcgcc actttcccgg aaggagccgc cgtacgggat tattacagcc agcaaattgc
      481 gatagtgaag aacggtcaaa taacgttaca acccgctgct accagcaacg gtttactcct
      541 gctggaacgg gcagaaactg acgcctctgc ccctttcgac tggcataacg ccacggttta
      601 ctttgtgctg acagatcgtt tcgaaaacgg cgatcccagt aatgaccaga gttacggacg
      661 tcataaagac ggtatggcgg aaattggcac ttttcacggc ggcgatttac gcggcctgac
      721 caacaaactg gattacctcc agcagttggg cgttaatgct ttatggataa gcgccccatt
      781 tgagcaaatt cacggctggg tcggcggcgg tacaaaaggc gatttcccgc attatgccta
      841 ccacggttat tacacacagg actggacgaa tcttgatgcc aatatgggca acgaagccga
      901 tctacggacg ctggttgata gcgcacatca gcgcggtatt cgtattctct ttgatgtcgt
      961 gatgaaccac accggctatg ccacgctggc ggatatgcag gagtatcagt ttggcgcgtt
     1021 atatctttct ggtgacgaag tgaaaaaaac gctgggtgaa cgctggagcg actggaaacc
     1081 tgccgccggg caaacctggc atagctttaa cgattacatt aatttcagcg acaaaacagg
     1141 ctgggataaa tggtggggaa aaaactggat ccgtaccgat atcggcgatt acgacaatcc
     1201 tggattcgac gatctcacca tgtcgctagc ctttttgccg gatatcaaaa ccgaatcaac
     1261 taccgcttct ggtctgccgg tgttctataa aaacaaaacg gatacccacg ctaaagccat
     1321 cgacggcttt acccctcgcg attacttaac ccactggtta agtcagtggg tccgcgacta
     1381 tgggattgat ggttttcggg tcgataccgc caaacatgtt gagttgcccg cttggcagca
     1441 actgaaaacc gaagccagcg ccgcgcttcg cgaatggaaa aaagctaacc ccgacaaagc
     1501 attagatgac aaacctttct ggatgaccgg tgaagcctgg ggccacggcg tgatgcaaag
     1561 tgactactat cgccacggct tcgatgcgat gatcaatttc gattatcagg agcaggcggc
     1621 gaaagctgtc gattgtattg cgcagatgga tacgacctgg cagcaaatgg cggagaaatt
     1681 gcagggtttc aacgtgttga gctacctctc gtcgcatgat acccgtctgt tccgtgaagg
     1741 gggcgacaaa gcagcagagt tattactatt agcgccaggc gcggtacaaa tcttttatgg
     1801 cgatgaatcc tcgcgtccgt tcggtcctac aggttctgat ccgctgcaag gtacacgttc
     1861 ggatatgaac tggcaggatg ttagcggtaa atctgccgcc aacgtcgcgc actggcagaa
     1921 aatcagccag ttccgcgccc gccatcccgc aattggcgcg ggcaaacaaa cgacactttc
     1981 gctgaagcag ggctacggct ttgttcgtga gcatggcgac gataaagtgc tggtcatctg
     2041 ggctgggcaa cagtgacttt tccggcttcc cgttcgtcag tacctcggga agccgccaac
     2101 caggataaaa tgtcagccct aatcagcgtt gcaggataaa gcaccgctca ctcttcaaca
     2161 gaccgatttg caccccagca aatgtagcgt tattgttacc ttccttgcta cagagttcga
     2221 cagatatccc gctatgacat tctccctttt tggtgacaaa tttacccgcc actccggcat
     2281 tacgctgctg atggaagatc tgaacgacgg tttacgcacg cctggcgcga ttatgctcgg
     2341 cggcggtaat ccggcgcaga tcccggaaat gcaggactac ttccagacgc tactgaccga
     2401 catgctggaa agtggcaaag cgactgatgc actgtgcaat tacgacggtc cacaggggaa
     2461 aacggagcta ctcacactgc ttgccggaat gctgcgcgag aagttgggtt gggatatcga
     2521 agcacagaat attgcactaa caaacggcag ccagagcgcg tttttctact tatttaatct
     2581 gtttgccgga cgccgtgccg atggtcgggt caaaaaagtg ctgttcccgc tggcaccgga
     2641 atacattggc tacgctgacg ccggactgga agaagatctg tttgtctctg cgcgtccgaa
     2701 tattgaactg ctgccggaag gccagtttaa ataccacgtc gattttgagc atctgcatat
     2761 tggcgaagaa accgggatga tttgcgtctc ccggccgacg aatccaacag gcaatgtgat
     2821 tactgacgaa gagttgctga agcttgacgc gctggcgaat caacacggca ttccgctggt
     2881 gattgataac gcttatggcg tcccgttccc gggtatcatc ttcagtgaag cgcgcccgct
     2941 atggaatccg aatatcgtgc tgtgcatgag tctttccaag ctggggctgc ctggctcccg
     3001 ctgcggcatt atcatcgcca atgaaaaaat catcaccgcc atcaccaata tgaacggcat
     3061 tatcagcctg gcacctggcg gtattggtcc ggcgatgatg tgtgaaatga ttaagcgtaa
     3121 cgatctgctg cgcctgtctg aaacagtcat caaaccgttt tactaccagc gtgttcagga
     3181 aactatcgcc atcattcgcc gctatttacc ggaagatcgc tgcctaattc ataaaccaga
     3241 aggagccatt ttcctctggc tttggtttaa ggatttgccc attacgaccg agcagctcta
     3301 tcagcgcctg aaagcacgcg gcgtgctgat ggtgccgggg cacaacttct tcccagggct
     3361 ggacaaaccg tggccgcata cgcatcaatg tatgcgcatg aactacgtac cagagccgga
     3421 gaaaattgag gcgggggtga agattctggc ggaagagata gaaagagcct gggctgaaag
     3481 tcactaaaaa ttgcctgatg cgctacgctt atcaggccta catgatttct gcaatatatt
     3541 taatttgcaa gaatttgtgg gccggataag gcgtttacgc cgcatctggc ataaacaaag
     3601 cgcactattc cggcgttgag aaacgccgga aaaacgtttc aaacaggctg ctgccgtagc
     3661 cgatgccgct gcactttctc gacatccatg cactgcaacg cctttgtcgg gcaggcttca
     3721 acacaggccg gtcccgtctc ccgatgccag cacagatcgc acttaatcgc cctcgccttt
     3781 ttgcgcgaag aaacgacctc catcgcacca aacgggcaag ccagcataca gcttttacag
     3841 ccaatgcaac gtgtttgttc aacgaaaata tgcccatgtt cgcggcttat cgcgtcaaca
     3901 gggcagacat tcgcgcacgg tgcatcttca cactgatgac aggctactgc cgtggtccag
     3961 ctgtggtctt taatgacacg aatacgggaa ataaactcgt ctggtgacaa cgcagcgcaa
     4021 tcctgattct cctgatgcga cactgcgcaa gccacttcac aggtacggca accgatacat
     4081 ttcgtcgcat ccgcaataat aaaccggttc atgatcactc ctttgcacta aaacagcaga
     4141 gtgccagaga gcgcgtccct actgctttga tccaacgaag gaaatgttaa agttttggca
     4201 cttgtccaaa gcgttatgtg atcgcgccct ggtcgtccct gacagtaaat cccagttcat
     4261 tagaaatagc ctgcgcggtt tcacgcagtg gtttcaggag atttttctcc cccacctgtt
     4321 tcagacgtga tgtcgaaagc gaaatcgccc cggcactacg aattgatatg ttccttgctg
     4381 taacgccgct tccacgctgc tggcgttaaa ccggtgtgtt tgcgaaaaat ctgccggaaa
     4441 tagccaacgt cattaaaccc gcacaacgcc gccacttccg tgagcgatcg cgcttcgcta
     4501 attaacagct tttcggcttc gcgcacccgc tggcgatgga ttgcttctgt taacgtcaga
     4561 tgaaacaccc gccggtagac gcgcccaaga taatcggcat tgcagtacag ctcgcgggcc
     4621 agaagcgccg tagaaagcgg gcgatgatat tgcgtgcgga tcagtttctg cgctttccag
     4681 gcaagggtgg tgcctgcgcc atcggcctgt tcatcaagac gcgcgtcacg ggtgagctgc
     4741 tgcaaaatga gcagcacaat aaactccagc gccggagtgc gttgatgctt ttcctgttcg
     4801 cttaagaact ggcgaaacag ggccaccagc gcctgcggat cgacaacctt cgtttgttgc
     4861 ggcacgctaa gatgagtaag ccagcgaggg tcgttgtccg gcgctttaac ctcaaaatgc
     4921 agccagtaaa atttgagatc ggcgggaaaa tcctccaccc caacatgttg tcggttcggc
     4981 cagagtaata aactctctcc cgcatcgacc agaaatatcc gttcttcttc ctgaatcgtt
     5041 aatcgacctt tttcgacaaa gataatttcc catgactcca gccgccttgc agaatgcctc
     5101 cctacgccac gggaaataaa taatccacca ttttgcactt taatcggtaa cgtcatggat
     5161 aattcgagca ttgatatgta atttcccgct tattcattaa tgattaaaca cattaccttt
     5221 tgattattaa tcaaaaacat taatttcacc tgatccattt cacactttta aattcaggtc
     5281 ggaatcgtca ccttttttta ctcttttctc gccttgttga tattcccgtt cacggcagaa
     5341 ttaatcaggt catttcaata aaactaaaaa ttgctcccta ttcacgagga atatctatga
     5401 cctctacacc gattactacc gctgatatcg ctaaaggtaa aattgacgat gcgttatctg
     5461 tacgggaaaa aataggctac ggcctgggtg acgcaggcgg caccgtaata acttgcctga
     5521 tcatgaattt tctcaccttt ttctacaccg acgtttttgg attaactccg gcgctggttg
     5581 gcacgctgtt tattgcactg cgcgtgtttg atgccatctc cgacccggtg atgggcgtca
     5641 ttgccgaccg gacgcaaagc cgatgggggc gctttcgtcc gtggcagcta tggattgcca
     5701 ttcccatcgg cattatcggc atcctgacgt tcaccgtgcc agatgccagc atgggagtaa
     5761 aaatcgcctg ggcgttcggt acttacctgc tcctttcagt cggttatacc gccatcaacg
     5821 taccgtactg cgcgcggatc aacaccatga ccacccgcca caatgaagtg atctcctgcc
     5881 agtcctggcg attcgttctc tgcggcgtag cgggatttct ggtttcggta ggcttaccgt
     5941 ggatggtagc tctcttcggt cagggcaacg ctgcacgcgg ctatcaactg ggcgtcgggg
     6001 tattgtgcgc cattgccgtg gtgatgttcc tgtgctgttt cttctgggtt cgtgaacggg
     6061 tgccgctctc cacaatgggg aaatttaccc tgcgcgaaca tcttgccggg ctgcggaaca
     6121 acgaccaact gctgctgatg ctggtcatgt ctttcctgct gattaacgtc tttaacattc
     6181 gcggcggtgg gtatatgtac ttcattacct acgtcttaca aggcagcacg ggctacacgt
     6241 cgctgttctt caccatggtc accttcgcct ccattatcgg ctcggtgatt gtcagcccgt
     6301 taacgcggcg tttcgatacc gtcaaaattt attactacac caacctgctc ctcgctgcac
     6361 tggcggtgtt gatgtggttc ctgccctccg gcccggctta tcaaacgctg tggctggcgg
     6421 tgatcctcgg taatggcgtg attcttggct tcacattgcc actgcacttc tcattgatgg
     6481 cctttgccga tgactacggc gagtggaaaa cccacgtacg ttcttccggc atgaacttcg
     6541 ccttcaatct gtttttcatc aagctggcct gggcctccag cgccgggatc atcagcctgc
     6601 tgtttatttt tgtcgcctac cagcctggcg tggaaaacca gaccgccagt tcgcttggcg
     6661 ggatcgcggc gatggaaaca ttactgcctg cgctattcca cctgctgctg gcaggggcga
     6721 tccgcttttg caaactcaat aatcctatga tgtcacgcat agctaccgac ctgcgtcagc
     6781 gtcatgtaca gccttaagga gcacaagatg aacatttcgg aagtcgatct gcataaactg
     6841 acggtcagcg atccgttcct cggtcagtac caacaactgg tccgcgacgt ggtgattcct
     6901 tatcagtggg atgccttgaa cgatcgtatc ccagaagcgg aacccagcca tgcgattgaa
     6961 aactttcgca ttgccgccgg acttcaggag ggtgaatttt acgggatggt gtttcaggac
     7021 agcgacgtcg ccaaatggct ggaagcggta gcctggtcgc tgtgccagaa gccggacgcc
     7081 gaactggaaa aaaccgccga cgaggtaatc gaactgatcg cctccgccca atgcgaagac
     7141 ggctatctca atacttactt tacggtaaaa gcacccgaag aacgctggag caatctggcg
     7201 gagtgtcatg aactttactg cgcaggtcat ctgattgaag ccggagtcgc cttcttccag
     7261 gccacgggaa aacggcgctt gctgggggtc gtttgccgcc tggccgatca tatcgacagc
     7321 gtatttggtc cagatgaaag taagttacac ggttatcctg gtcacccgga aattgaactg
     7381 gcactaatgc gcctgtatga agtgactgaa gagccgcgct acctggcgct gacgaactat
     7441 tttgtcgaac agcgtggtgc gcaaccgcac tattacgacc aggaatatga aaagcgcggg
     7501 cagacatcgc actggcacac ctacggcccg gcgtggatgg tgaaagacaa agcctacagc
     7561 caggcacatt tgccccttgc gcaacagcaa accgccatcg gtcacgcggt acgttttgtc
     7621 tatctgatga ccggcgtcgc gcatctcgcg cgtttaagtc acgatgacag caagcgtcag
     7681 gactgcctgc ggctgtggaa caatatggcc cagcgtcagt tatatattac cggcggcatc
     7741 ggctcacaaa gcagcggcga agcgttcagc agcgattacg atctgccgaa tgacacggtt
     7801 tacgccgaaa gttgtgcttc catcggcctg atgatgttcg cccgacgaat gctggaaatg
     7861 gaaggcgaca gtcaatatgc cgatgtgatg gagcgcgcac tgtacaacac tgtgctcggc
     7921 ggcatggcat tggatggcaa acatttcttc tatgtgaatc cactggaagt acatccaaaa
     7981 tcgctgaaat tcaaccatat ctacgatcac gttaaaccga tccgccagcg ttggtttggc
     8041 tgcgcttgtt gtccgccaaa tatcgcccgc gtgctgacct cgattggtca ttatctctac
     8101 acgccgcgtg aagatgcgtt gtatatcaac atctacgcag gaaacagcat ggaagtgccg
     8161 gtagaaaatg gcacgctgcg cctgcgggtt agcgggaact atccgtggca ggagcaggtg
     8221 acgattgcgg ttgaatcgcc ccagccggta cgtcatacgc tggctttacg tctgccggac
     8281 tggtgcacac agccgcagat catattgaat ggggaagagg tcgagcagga tattcgtaaa
     8341 gggtatttgc acattacccg cgaatggcag gagggcgata cgctgaatct gactttgccg
     8401 atgccggtac gccgcgttta cggtaacccg ctggtgcgtc acgtcgccgg aaaagtggcg
     8461 attcagcgcg gcccgctggt gtattgcctg gaaaaggccg acaacggcga gtcactgcat
     8521 aatctgtggc tgcccaccga tgcgccattt acgacatttg aaggcaaggg attgtttagc
     8581 cataagatct taatccaggc accgggttac cggtatgaac agagcaatcc agagcagcaa
     8641 ccgctgtggc attacgacag cgcgccagcc aaacgccaga cgcaaactct gacctttatc
     8701 ccgtggttta gctgggccaa ccggggtgaa ggcgaaatgc ggatctgggt gaatgaggaa
     8761 aagcattgcc atccgtaggt tggataaggc actcgcgccg catccaacgt aaggagcaca
     8821 ttgcctgatg cgacgcttaa cacgtcttat caggcataca agtcggtgcg agagcgtagg
     8881 ccagccgcac cgacatccac cggtgaatta aaaacgccag ctcacgccgg tgacaaaggt
     8941 aaaactctca ttacgatcaa tcatcgggct gtcggtaatc tcttccggca gctcgctgta
     9001 tcctgcgctc gccatcaata cgacgtgctc tcctatcggg tattttgccg tcaggctgac
     9061 atagggcacc caggcatcct gcgcggaata acttgccaga ccgctacgac gggactcact
     9121 ctctgaaatg ccatagtaat agtcactgaa attctcgtca taatagagaa cgcccagcgc
     9181 aggtgtcagc gacagacgac ctatctgcat tttgtggaat accgatagct cccccaccca
     9241 gccgttgctg ttatccagaa cgtccgcagc tgcagaggct ttcacggttc cccaacgctc
     9301 gtggtgatac caggcaaccc ccgccatagc cgtactatca cgcttatcaa gctgttgcat
     9361 ggcataatcg tcattatcgg cagggtcaaa ttccagcggc atccaggatg cggtcaggct
     9421 aagttcattt ttttcacttt gcgacagaat aaaacctaac gtggtctgac gaacataaaa
     9481 agaatcacct tcataactaa tcagcggaat tgcctttgta ttttcattat aaccgcgata
     9541 aggcgattca ttataagccg caccagcacc aatggataat tcagaagcag ttgcgcttgc
     9601 cataaaaggc aacgcaaata acgccacaat attgcggtta attaacatgt ccggtattcc
     9661 attttaaaat aagataaaaa agagtcggca tagtaatgct tacaatttct ccggtgcaag
     9721 attcatattt atatagtaat atataaaatt atatataatt gggctgttgc gatgacaaaa
     9781 ttacaactta agtaccggga gttaaaaatt atctcggtaa tcgctgccag tgaaaatatc
     9841 agccatgccg cgactgtact tggcatcgca caggccaacg tcagcaaata tcttgctgat
     9901 tttgaatcaa aagtgggttt aaaagtcttt gaccgaacca cccgacaact gatgctcaca
     9961 ctttttggca ccgcgctatt accgtacatc aacgacatgc tggacagaaa cgagcagctt
    10021 aataatttta ttgcagatta taagcatgag aaacgtggtc gggtcaccat ctacgcccca
    10081 accggcataa ttacctattt atccaaacat gtaatcgata aaattaaaga tatcggtgat
    10141 atcaccttat cgctaaaaac ctgcaatctt gagcgtaatg ccttttatga aggcgtcgaa
    10201 tttcctgatg attgtgatgt tttaatcagc tatgccccac caaaagatga atcgctggtt
    10261 gccagtttta ttacccaata tgccgtaacc gcttatgcca gccagcgcta tcttgagaaa
    10321 catcctatta gccgtcctga cgaactcgaa catcactcct gtattttgat cgactcgatg
    10381 atgatcgacg acgcgaatat ctggcgcttc aacgtcgccg ggagtaaaga ggtgcgggat
    10441 tatcgcgtga agggaaatta cgtttgcgat aacacgcaat cggcgctgga gctggcacga
    10501 aatcacctgg ggattgtgtt tgcgccagac aaaagtgtgc agagcgatct gcaagacggc
    10561 acgctggtgc cctgctttca gcacccgtat gaatggtggc tggatctggt ggctatcttc
    10621 cgtaaacggg aataccagcc ctggcgggtc cagtatgtgc tggatgaaat gctgcgtgaa
    10681 attcgccacc agcttgctca gtcgcagcaa ctgcggcctg aacaagcggc ggaaagcgaa
    10741 gattaatgat cgagataaag gtaatgcacc cagctggtca tgcgtaataa cactttgcgc
    10801 atcaccgaga catggctgaa atgatcagag tagaccgcca cctgagcgta aataccgtct
    10861 ggtaaagcgc tcaaatcagt gtgctcattc agttcaatgg tcgcgataac gccatctgaa
    10921 cccggcgctg tgtttaacgt ctgtaaggtg ccggtcgact gataagctcc gccgggaaca
    10981 gctggactaa tggctgccag cttaccgctg aacactttgc ctggcagagc gttaaatacc
    11041 acttccgcat catcgccggg agccaggcgc agcaaggagt tctgacggaa ctgcgccacg
    11101 atttgtcgtt tctgatcggg tataaacacc atcaccggac gtagcggcag cgacgcggca
    11161 taggtacccg gacgaatcag cacttgggtc acataaccat cgctcggcgc acgcactatc
    11221 gtctgctcaa ggttatattt tgcttccgcg agctgcgctt tcaggctggc gatttgagaa
    11281 tgttcaccca acaccaggct atccagctgg ctctggatct gtttttgttc cgccgccgat
    11341 gactttacgg aggcttcctg cgccagataa ttttgccgtg ccacatcaat atcgcgttct
    11401 gaaaacgggt ttactttcgc ctggctgccg cgtgcgtaac gctggtactc cttagcgaat
    11461 ttatcccgcg tggcctttgc cttctgagta ttcgccgcca tctcatctaa ctctgcgccc
    11521 aacgcccgct gtttatgttc tgcggtaacg atatccgcca tcagccgatc cacccgcgcc
    11581 tgataacgcg tcgggtccag tcgaaatagc acctcacctt ttttaatcag cgtatttttc
    11641 ttatccgtca cttctatcac cacgcctgtc acctgtggga caacaggaat agaaataacc
    11701 gctttttgcg ctttaaaggt atacggatgg ttatagttca ttaacagaat tagaccgctg
    11761 acaataaata ttccacccag ggccgctgtg ggaatggtcc atttatttac gggaattttg
    11821 aagatcttaa acattgccca tgcaaaagcc acataggtca aaataatcag taaatccata
    11881 agtattactc ggcagaagag gatttgatgt cggcgagctg tttctccagc ccggcaatgc
    11941 gctgttgcag ttgcataacg gatgaatcat ggctttgcat tccccatccc cgctccgggc
    12001 ggtaaagcgt ggcccaaatc cacaaaaatg gccagataac atgcagcgta aaaagactca
    12061 cccaaccagc aacatgaatg gcgtcggcat gaggatgatt acgtttttta gcaatcaggt
    12121 agggaatatc atgtaaaata ataatcccat agaatatcac cagaaataca aaaataagca
    12181 ctcccagtgc aaaatagtcc aggaacatat ttccctcaga gaatataaaa aagaacaatt
    12241 aacgcatatt atgaaatgcc atgatgcaat gcaatttcct tatttatata acgacatatt
    12301 gttttttata tactttctga agtgtgctgc gtggcttttt aacaggtcga ggcttatcat
    12361 attgaaataa tggaactatc agccaaacta atgaataact tcagtaaaac atccacaatg
    12421 cagattaagc gtattaaacc ttcaataatc taccgtttat tgctgattgt cctcggaata
    12481 ccaatggtga tttacggtct ggtttgcccg ctcaccatcg aaacgcgaga ttaaactcct
    12541 ggcaaaacac gccccagaaa cgcacttttg ttggcgtagt ctgggttatt gcacacgtag
    12601 gtttaaaacg taaattgtaa caacgtgcgc tttgtttatg ccggatgcgg cgtgtacgcc
    12661 ttatccggcc tacggtcagg tccccatagg catgataaga cgcgtaagcg tcgcatcagg
    12721 caattaatac ccgatgcgac cagcttctta catcagaaca gccccaacgg tttatctgag
    12781 tagctcacta gcaggcactt ggtttgctgg taatgctcca gcatcatctt gtgggtttcg
    12841 cgaccgatac ctgattgttt gtagccacca aacgccgcat gtgccgggta agcatgataa
    12901 cagttggtcc acacgcgccc agcctgtatg ccgcgcccca tcttataggc cagattaccg
    12961 ttgcggctcc agacgcccgc gcccaggcca tattgcgtat cgttcgccag ctccagcgct
    13021 tcttccatcg ttttgaaggt agtcaccgcc agcaccgggc caaaaatctc ctcctggaac
    13081 acgcgcatat tgttctgacc aaacagaatc gtcggttcga ggtagtagcc gtctttcagt
    13141 tcaccttcca gcagcttgcg ccgcccgccg gtgagcacat cagcgccctc ttttttaccg
    13201 atatcaatgt agttgaggat ggtttccagt tgcccgtgag aaacctgcgc acccatttgc
    13261 gtcacgctgt cgagtgggtt accgctgcga atgctttcga cacggcggat ggcgcgttcc
    13321 ataaagcgtt cgtagattga ctcctgcact aaggcacgac tcggacaggt gcaaacttcg
    13381 ccctggttaa aggcaaacag tgcaaagcct tccagcgctt tatcgaaaaa ggcatcttct
    13441 tcatccatca catcagcaaa gaagatattt ggcgacttac cgcccagctc cagcgtcacc
    13501 ggaataatgt tttgcgtggc gtattgcata atttgttgac ccacttccgt tgagccggta
    13561 aacgccactt tggcgatgcg tttcgaggtc gccagatatt cgccaatttc cccacctgca
    13621 ccgttgacca cgttcaccac gcccggcggc agtaaatcac cgacgatttc cattagcagc
    13681 agtacagaaa gcggggtaag acgtgcgggt ttcagcacca cacagttgcc cgccgccagc
    13741 gcgggagcca ttttccagct cgccatcagc agcgggaagt tccacgggat aatctgcccc
    13801 accacgccta acggttcatg gaaatgatag gccacggttt cgctatcaac ttcactgatc
    13861 ccaccttcct gcgcccgaat acacgaggcg aaatagcgga aatggtcaat cgccagcggt
    13921 acatccgcag cactggtttc gcgaatgggt ttgccgttat cccaggcttc agctgtcgct
    13981 aacagctcga ggttttgttc cattcgatcg gcaatcttaa acagaatcgc cgcgcgatcc
    14041 tgcaccgagg tgtgcgccca tttatctttc actttgtgcg cagcatccag cgccagatcg
    14101 atgtctcgtt tgcccgaaga cgccacttcg cacagcagct gcccggtcac cggcgtcagg
    14161 ttctggtaat actcaccatc ggcaggggct acccattcgc cgccaataaa gttgtcatag
    14221 cggtttttta acttgagggg gaaaccatac tcgccgggct taatctgtgc tgaaggggga
    14281 ttattggtca tgatcgtctc cttgcggtgt gaggtataac aagggtagac gtggctggcg
    14341 aaatcttcgc cagtcggtaa cagcttttcg acggctatca cgaatttacg ggcaagggtt
    14401 acagcggata atgcgcgaag tacacggctt gctggtcatt aattttcttc tcttgcgcaa
    14461 acccttcttt aacaattgtc cgtaaatcag ctcgaaccgt attatcagag acgcccaggc
    14521 tcgcgctaat ttcagcaact gtgtactctt tccccggatt ggcgagcatc acattcagga
    14581 gcgttacctg gcgcggattc aggcgtttaa gcacaccaga gctaaacagc acactatcga
    14641 gttttgcgct tcgttgtgta atttcatcga tatgctgctg ccagttttgc agagcacgtt
    14701 taatgacacc cgcctgatat tcaaggaaat aagtcagatc cataccatct gattcagtgt
    14761 actggtagct ggcggcatat ttgaccggtg cagcatgaag caaacggctg atggaaatat
    14821 atttaaagac atcgtagccc gatttcagca tataccagta aaacagcgcc cgactggtgc
    14881 gcccgttgcc atcccgaaac ggatgttcat gggccagcat aaaatgcaga atgcaggcac
    14941 ggaccaacgg atgaatatac ccttcatggc tgtttaacca ctggcaaact ttttccagcc
    15001 gttccggtaa caatgccgca gcgggcggtt gatgcactat attgccatca tagtcggcga
    15061 tcacaacatc atctgtttca cgaaattctc ccggacgata tttcgcgtca ttgattccac
    15121 ccataccaat ggcatgtaac tggcgaataa gcgcaggtgt cagcggttct gcgagtaagt
    15181 gcggaatttc cgccatcagc cgggcatttc ccgcaatcat atgctcgtct tcagtgcgtg
    15241 gcgaccgtcc tgtttccagc atttttctcg ccaccagcgt tgtagtattc gcaccttcca
    15301 gctgggagct ggtgatcggc tcttccaggc gtaactggct taactcggct ccggccccgc
    15361 gtaaccgttc agtctgatct ttcagcgcca gcgcggtagc acgtttgtcg acaagctcgc
    15421 acgcttctgc aatcaccggg gtaatgttga atccggcctg ttcacccgct tcattgcaat
    15481 aattaattcg ttggatcgcc gcgttgcgcg ccagacgggt aagcgtccac gccacatcga
    15541 cgttttctcc ttttttgatg cggtactgca actcatcaaa aggcagataa cgtccttttt
    15601 catcggtaac ccggttaaag cgcaggtggg cctggagttc ctcacccgat aagtgtgata
    15661 aaggaatcag cgtgggaggt gatttgatag ccatttgctt atctcaaaaa agcgcaatat
    15721 tgagatagtg tatagcagga aagagaaact atctcaaaaa aagcaagttt tgagatagtt
    15781 gaaaaaatgg ctgacaattt gccagccgtt gtggaaatga tgattacatc gctgcgcgat
    15841 aaatcgccac aatttcttcg tgagttgcct ggatcgggtt agtaaagcca caggcatctt
    15901 tcagggcatt agccgccaga accgcgaaat cttcttcttt cacgttcagg tcgcgtaggc
    15961 ctgccgggat atccactttc ttcgccagtt cacggatggc gttaatgcag gcttcagcac
    16021 cttccgcgtc gtttttacct gtcacgttca cgcccattgc agcggcacag tcacgcagac
    16081 gtgcagcggc gactttgctg ttgaatacct gaacatgcgg cagcagaacg gcgttacata
    16141 caccgtgtgg caggttgtag aaaccgccca gctggtgcgc catcgcatga acataaccca
    16201 gagaagcatt attgaacgcc ataccggcga ggaactgggc ataagccatt gcttcacgcg
    16261 ctttcgcatt actgccatct tcaacggcta acggcaggtt ttcggcaatc atggtcacgg
    16321 ctttcagtgc acaagcgtca gtgatcggcg tggcggcaat agaaacatac gcttcgatag
    16381 cgtgcgttaa ggcatccata cccgttgcgg cggtcagtga cttcggcata ccaatcatca
    16441 gagaggagtc attgacagaa agcagcggag tgacatgttt atcaacaatc gccattttga
    16501 tatgacgcgc ttcgtcagtg atgatgcaga aacgggtcat ttcagaggcc gtacccgccg
    16561 tggtattgat ggcgatcatc ggcagctgcg gttttgcaga gcggtcaacg ccttcgtaat
    16621 cgcgaatatc gccgccattg gctgccacca gcgcaatacc ttttgcgcag tcgtgtggag
    16681 aaccaccgcc taaggagatc acgctatcgc aattattctc tttaagtaat ttcaaacctg
    16741 cggcgacgtt ttccgtggtg gggttaggtt gggtgccatc ataaataacg ctaaaaatat
    16801 tgcgttcttc cagtgctttt tgcacatcgc ccgccatacc taatttcgtt aacatactgt
    16861 cagtgacaat taaggtacgg gtaaatccat aatctgccat catattcatt gcatcagtca
    16921 atgaatcagc gccgatgaca ttcacagaag gaataaagaa agttgaagat gccataacac
    16981 actcctgaaa gtgtaaaaaa gggtgggcag cttactgagg attttcattc gaaaacatga
    17041 tcattattat taattatcaa aattaatcta ataaaaagca cattgtattc attaaacaat
    17101 gtgctttttt aaaagcataa gaaatttgta ttaatttatt ttaaaaatta attaatcatt
    17161 tccttatttt tccggaaata ataatgcgtc gcgcaataaa tgatcatttc cacgacgacg
    17221 cgtaaagcca atgcggtcaa aatattccag aatctgaatt gccagctttc ggcctacgcc
    17281 taagcgatcg cggaaatccg ccgcgcaggt tgaaccacac tcctgatcga gatcgcggat
    17341 catattggca aattcgacaa tccgatcgtt acggtaataa cgatctttga cgatcgcggt
    17401 aattattcct tgctgcgccg cctggcgtag cgtcaggcgc attgcctgct cgtcggtacc
    17461 cgtctctttt gccagatcac gcacccacca cggctcgtca ccaaacagcg gctctgcttt
    17521 ttgccagatg gcctgctgct cttcgctgaa gcccgctttg tgatccggca gatgcagcca
    17581 gccgtgatga ctgaggatgt cgccgctttc gcgcatcttt tcaatcagca acagtactag
    17641 cgcttcatct tccattggca acgccatacg tcgcagacgt tcgcgcccag gcccaggttc
    17701 atcgcgatgt tgctcatgat aagtcgctaa tgtgtcgaga attttccgtt gccagcgagc
    17761 ggcaaccggc gcattcaaca agctataacc agcctgaata tagccaggct gttgcagcag
    17821 ttcgcgcatc ccttcgccgt tgagctggcg cgcccaggcg aaatcaggaa gattaaccgc
    17881 gccgcgttgc aggtgaacgg ctaacgcttc gctatcattc tgtgccctgg cgagggaagc
    17941 caggcattgc agatactctg gtttacgttt accgcgacgc ggcgggttaa gcatcacgac
    18001 gcgcgctccg gccagcgtgt tgcgggcaga gatatcgcgc aataccaggc ggtcgttatc
    18061 tgccagccat aacggggggt cgaagaccag ttcagcaagg ttatcttcca gcagtgaaac
    18121 gcgtcccgtg acgtggctgg cggcgtggtg aatatgcagc ggctgccact gggtcagcgg
    18181 tgtatgggtt tgaagctcga caatcacccg tgtgaacggc tctggcggca catcggcaag
    18241 cagccagtcg ccacggttaa tctgctcttt ttccgcatca cccgcgatgt taagcgcgat
    18301 acgctgcccg gcattggcgg tttctgttgg ctggttttgc gcatgcagcg cacgtacacg
    18361 catcggttta tttacaccag tcagccagag tgaatcgcct accttcactt ccccgcttaa
    18421 cgccgtaccg gtgacgacca gcccggcacc ttttacggta aatgcgcggt caatcgcgag
    18481 gcggaaacta tgttggctgg cgtgctcgcg ttccggcaac tgaagcagat gctcgcgcag
    18541 ggcatcaatt ccccgacctt cggttgctgc ggtgataaac agttttgcct cagcaaaacc
    18601 gtattcccgc agaacctcct ttacctggcg ttcaacctca tcaacacgcg cttcgtctac
    18661 gcgatcggct ttggtcagcg ccactgtcag catcgggtta ccggtcagct gcaaaatcgc
    18721 cagatgctca cgggtctgtg ccatcacgcc gtcatcgcac gccaccacca acagcgcgtg
    18781 atcgatacca ccaacgcccg ccagcatgtt ggaaagaaac ttttcatgac cgggtacgtc
    18841 gataaaacca ggcacgcgac catccggctg cggccagtag gcatagccga gatcgatggt
    18901 catgccgcgc tttttttctt ccggcagacg gtcagcattt acgccagtaa tcgcctgcaa
    18961 taaggttgtt ttgccgtggt caacgtgtcc ggcagtcgca ataatcattt caacaacatc
    19021 tccaaaaacc gttgctcatc ttcaaggcag cgtaaatcca gccacaatcg tccgtcataa
    19081 atacgaccaa tcaccggcac tggcaattca cgccagcggg cggctaatga ctcaaggtgg
    19141 ctaccgcgtc catcatgggg tgtaaacgtt aatgccgcgc tcggcaggcg atcaaccggc
    19201 agcgaaccac tgccaatctg cgaaagacat ggcataacct gtaccgcaaa ctccgcgccg
    19261 taatgtgcgg caaggggagc ctgtaaacgt tgtgcctgga tttgaatgac ctctgcgctg
    19321 cgggtaagca ggcgcagggt cggtaatttt tcactcagag cttcagggtg taaataaaga
    19381 cgcaacgtgg cttccagcgc cgcgagggtc attttatccg cgcgtaatgc acgcttcagc
    19441 gggtggcttt gcaggcgggc gatcatctct tttttaccaa cgataatccc tgcctgcggc
    19501 ccgcctaaca acttgtcgcc ggagaagctc accagactga cgcccgccgc aatcaactcc
    19561 tgcggcattg gctctttcgg caaaccgtac tggctaagat cgaccagcga gccactgcct
    19621 aaatcagtca ctacgggaac atccagctct ttgccgagcg ccaccagttc cgcttcatct
    19681 atcgctttgg tgaacccctg aatgctgtag ttactggtat gtactttcat caacagtgcg
    19741 gtattttcat tcaccgcctg acgataatca ttcgcgtgcg tgcggttggt ggtccctact
    19801 tcgtgtaggg tgcagcctgc ctgacgcata acatcgggaa tacgaaacgc gccgccaatc
    19861 tccaccagtt cgccgcgaga taccaccacc tcttttccgc tggcagtggc cgccaacatc
    19921 aataacaccg ccgccgcatt gttattgacg atacaggcat cttccgcccc cgtaatacgg
    19981 cacagcagct gcgccagcgc ccgatcgcga tgtccgcgtc cggcgtcgtc cagatcgtac
    20041 tcgagggtca ctggcgaacg catagcctgc gcaacggctt ccaccgcggc ttccgcctgt
    20101 aaagctcgcc caaggttggt atgcagcacg gttcccgtca ggttgatcac cggacgcagc
    20161 gcgctctgcg cttctttcgt caaccgggca tcgacttctt gcgcccagtt ttcacaccac
    20221 gcaggcagcg tctggctgcc acgaatcact tctcgcgctt cgtcgagcat ctgacgcaac
    20281 aattccacca cgcgggtgtg accataagta tcacgcaaag aaaggaagga gctatcgcgc
    20341 aataagcgat caatagccgg aagttgacta tagagggaac gcgtttcggt tgtcatagga
    20401 aacctggctg atcaaggccc tctcacacgg agaagggcgt ttaacataac cacggattgt
    20461 aacgtgagat gggtcaggag gacatatcgc gcatcaagcc tttggcggtt cggtgcgagc
    20521 aaaactttcg cggctaaaca ggttttcgac cagtttgact aagtgtgggc ggtcgacaca
    20581 ccaacctggc gcaacgcggc ggaaattgag atatccgacg gcacaggcaa tggcgatagt
    20641 cgccagattg accgtatcgg ttttgagtgt gccatcgacc agatatcctt ccagcacatc
    20701 cagactgcgg ttgattttct cccgctggcg taacaattca tcttcagact gctgcgccgc
    20761 tggacgcgcc tgttcacgca ccgataccag cccggcatcc ataatgccat ccgccagtgc
    20821 ctcaattttg cgcacccgca acgactccag cggatcgcgc ggcaacatcg ccggagcgac
    20881 attcattaat tcaatatatt cagcgatgat cggcgaatca aaccagcatt cgccctcttc
    20941 ggtcaccagc accggcactt ttcctaacgg gttaaattgc gtcacgccgt tgtccgcgtt
    21001 atagggcagt tcattaataa attcgaaagt tatgcccttt tctaacaaca gaatagaaag
    21061 tttgcgtaca aacgggctgg tgtagctacc gacgagtttc atgccgagtc ctttgtgcga
    21121 ggaaaaatat cagtatggcc tggtgatggg aaaaagggca gaaaatgttg atggtttgtt
    21181 acttccaaat aaatcacata tttatcatgg tgatataaat attttcctaa ttgtttctat
    21241 ctgatggata tctcacttaa ggctttctta taaatctgta gggttttgcc tggaagcaga
    21301 caaataaccc gataaaacaa ggatgagcag atgagcggaa aaccggc
//
LOCUS       DAZKIP010000073        21295 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZKIP010000073 DAZKIP010000000
VERSION     DAZKIP010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.073"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(77..229)
                     /locus_tag="ACHOUK_002991"
                     /pseudo
     CDS             complement(77..229)
                     /locus_tag="ACHOUK_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(226..346)
                     /locus_tag="ACHOUK_002992"
                     /pseudo
     CDS             complement(226..346)
                     /locus_tag="ACHOUK_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(386..649)
                     /locus_tag="ACHOUK_002993"
                     /pseudo
     CDS             complement(386..649)
                     /locus_tag="ACHOUK_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            743..1092
                     /locus_tag="ACHOUK_002994"
                     /pseudo
     CDS             743..1092
                     /locus_tag="ACHOUK_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1729..2160)
                     /locus_tag="ACHOUK_002995"
     CDS             complement(1729..2160)
                     /locus_tag="ACHOUK_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity TolA C-terminal
                     domain-containing protein"
                     /protein_id="HFP9898394.1"
                     /translation="MVLRSIWVEEQYMGVKKIVILAILVSFVAGCAPLHPSNCHKTTA
                     LGSCSSGRWDDQDEWGEQARGIRDAINAKLDDPQKWKRKKCRLHMEFSRDGTALKIST
                     SNGDKAYCEAIKSAAHKAKFPAFNNPEVYRDFQKSGFDMRG"
     gene            complement(2228..4249)
                     /locus_tag="ACHOUK_002996"
                     /pseudo
     CDS             complement(2228..4249)
                     /locus_tag="ACHOUK_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4383..4508)
                     /locus_tag="ACHOUK_002997"
     CDS             complement(4383..4508)
                     /locus_tag="ACHOUK_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HFP9898395.1"
                     /translation="MLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK"
     gene            complement(4450..4599)
                     /locus_tag="ACHOUK_002998"
                     /pseudo
     CDS             complement(4450..4599)
                     /locus_tag="ACHOUK_002998"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5431 family protein"
     gene            complement(4773..5534)
                     /locus_tag="ACHOUK_002999"
     CDS             complement(4773..5534)
                     /locus_tag="ACHOUK_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFP9898396.1"
                     /translation="MSARSQALVPLSTEQQAAWRAVAETEKRRNQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELHGNRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFCVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWYARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEASLAAKE
                     AGHAVREMERWMVPNKLREVRDAATITRIARKQPE"
     gene            complement(5531..5965)
                     /gene="psiB"
                     /locus_tag="ACHOUK_003000"
     CDS             complement(5531..5965)
                     /gene="psiB"
                     /locus_tag="ACHOUK_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFP9898397.1"
                     /translation="MKTELTLNVLHTMNAQEYEDIRAAGSDARRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDTQGYSVNDIIHILMAEGGQV"
     gene            complement(6020..7978)
                     /locus_tag="ACHOUK_003001"
     CDS             complement(6020..7978)
                     /locus_tag="ACHOUK_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFP9898398.1"
                     /translation="MSVTESKAKTERKSSRKPAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELADSIKGVGLLQNLVVHALPGDRYGVAAGGRRLAALNMLA
                     ERDIIPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADTFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMEPVGFCREDAGTYRSLPEPEAVLTEAEE
                     ERLNELMARYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDVADNDYRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQSD
                     KSLALLAWTLCLNVFGSGAYSNPARIRLECEHYSLTSDAPSGKEGAAFMAMMAEKARL
                     AALLPDGWARDMTTFLSLSQEVLLSLLSFCTACSIHGVQTREYGHTSRSPLDTLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPRPQTDATERTDNLADAA"
     gene            complement(8044..8277)
                     /locus_tag="ACHOUK_003002"
     CDS             complement(8044..8277)
                     /locus_tag="ACHOUK_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFP9898399.1"
                     /translation="MSEYFRILQGLPDGPFTRKHAEAVAAQYRNVFIENDHGEQFRLV
                     VRNNGAMVWRTWNFEDGAGYWMNHVIRDFGIIK"
     gene            complement(8334..8786)
                     /locus_tag="ACHOUK_003003"
     CDS             complement(8334..8786)
                     /locus_tag="ACHOUK_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP9898400.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLVRAAGAQTQPEEGQQFSGQPQPQPPEGDDYGFSDDIPF"
     gene            complement(8812..9018)
                     /locus_tag="ACHOUK_003004"
     CDS             complement(8812..9018)
                     /locus_tag="ACHOUK_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP9898401.1"
                     /translation="MHIPQNFLPKSRQKVLRSAPGQSFPAVAVMPLAAKVLKKVMVNT
                     GGNKRQVPAKRQNRGSRNSKDDGG"
     gene            9088..9543
                     /locus_tag="ACHOUK_003005"
     CDS             9088..9543
                     /locus_tag="ACHOUK_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9898402.1"
                     /translation="MAPDTTTGRHPPQRREARFAKRRCPLHPGGLLPGCRERRPRRDR
                     GGATGTVAASPGFASGMEARQGGDSSAGSVHDSPPRQGDARKRHKQENNKTDSNQSSR
                     KKKNSSSSSSSSSSSSSSMNNNIISNRQMTGLKRQKPTAECPRNVRGGA"
     gene            complement(9629..10192)
                     /locus_tag="ACHOUK_003006"
     CDS             complement(9629..10192)
                     /locus_tag="ACHOUK_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFP9898403.1"
                     /translation="MHSQLKERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRVIINPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTDVPTMIIRLRA"
     gene            complement(10239..11600)
                     /locus_tag="ACHOUK_003007"
     CDS             complement(10239..11600)
                     /locus_tag="ACHOUK_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HFP9898404.1"
                     /translation="MTSAAITMTAPEAASPVQMYRATYSPDDNKLRLYAVSRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERGRSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLNYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            complement(11652..11882)
                     /locus_tag="ACHOUK_003008"
     CDS             complement(11652..11882)
                     /locus_tag="ACHOUK_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9898405.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            12399..12908
                     /locus_tag="ACHOUK_003009"
     CDS             12399..12908
                     /locus_tag="ACHOUK_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9898406.1"
                     /translation="MSRDPPQRREARFAKRRCPLHPGGLLPGCRERRPRRDRGCATGT
                     VAASQGFASGMEARQGGDSFAGSVHDSPPRQGDARKSHKQERNGKKRRKRKRKKKKNS
                     NRHGNHRKITSEKNIPDTSQTGRVTDDASAWPASLPAGVSLPGVFARADRRPPPPALY
                     HLFLLPCKM"
     gene            complement(12878..13069)
                     /locus_tag="ACHOUK_003010"
     CDS             complement(12878..13069)
                     /locus_tag="ACHOUK_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9898407.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(13066..13488)
                     /locus_tag="ACHOUK_003011"
     CDS             complement(13066..13488)
                     /locus_tag="ACHOUK_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFP9898408.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRHIAQDWNLSD
                     DELETVMQRLDDAFEYGADVSIVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            complement(13535..13960)
                     /locus_tag="ACHOUK_003012"
     CDS             complement(13535..13960)
                     /locus_tag="ACHOUK_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFP9898409.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADACGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            complement(14378..15154)
                     /locus_tag="ACHOUK_003013"
     CDS             complement(14378..15154)
                     /locus_tag="ACHOUK_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9898410.1"
                     /translation="MNENTTLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIY
                     VLLDAPRLATLLVNRHGGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLTEDEIRAVLAAVRDAVRSVSCRVAEDTRRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDV
                     LRIPDEPPRRWFDRGVLREVVREARNEIRSMADALAKIRK"
     gene            complement(15200..15754)
                     /locus_tag="ACHOUK_003014"
     CDS             complement(15200..15754)
                     /locus_tag="ACHOUK_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFP9898411.1"
                     /translation="MCLLNAWHARRCIAPIACSWRRIPKFWNWMRALKIEKETRMYGT
                     CETLCRELAAKYPGYTPLMLVIWSPEEIQALADGMDISLSDPEIRTVLARLEDIPEDQ
                     RIESGISSAAAMEIISNVSENRQVTVPAELLASLIQTAEQALWKREWAARDYGLAVPE
                     CVTRRQAVVNQARILLKNNTHENG"
     gene            complement(15648..15869)
                     /locus_tag="ACHOUK_003015"
     CDS             complement(15648..15869)
                     /locus_tag="ACHOUK_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9898412.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LARQTLYRANRLFMEAYTEILELDACFKD"
     gene            complement(15870..16553)
                     /locus_tag="ACHOUK_003016"
     CDS             complement(15870..16553)
                     /locus_tag="ACHOUK_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFP9898413.1"
                     /translation="MSRFIQGDCVRVMATIPGNGVDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKTYT
                     SKAAYVGYRHECAYILAKGRPALPQKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(16630..16935)
                     /locus_tag="ACHOUK_003017"
     CDS             complement(16630..16935)
                     /locus_tag="ACHOUK_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9898414.1"
                     /translation="MTETGGQPPVSFPVKDVAGLLFLLRRLTRRGRNAACGQCPASGR
                     ARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGIFSPSKRRRLRYSTVGLTRYRTR"
     gene            complement(16939..17841)
                     /locus_tag="ACHOUK_003018"
     CDS             complement(16939..17841)
                     /locus_tag="ACHOUK_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFP9898415.1"
                     /translation="MYFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QTTEDVRFEPCPGLTAAGRGVVSPENIAFTRWLTHLQDGVLLDEQNCLMLHELWLQSG
                     TGRRRWEELPDDARESITALFTPKRGDWCDIWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLSTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPCGYEVNISSQGDNFIQVDF
                     DTPWCQPESDVIAVLSRRFSCMLEHWYAGQGCNFCGWQRYERGELVDVLWGELEWSSP
                     TDDDELPEVTAPEWIVDKVAHYGG"
     gene            complement(18536..19507)
                     /locus_tag="ACHOUK_003019"
     CDS             complement(18536..19507)
                     /locus_tag="ACHOUK_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HFP9898416.1"
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGN
                     AITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQ
                     TPAFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSA
                     YERGQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSA
                     RSGDALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLS
                     SRHQFAPGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     gene            complement(19507..20673)
                     /gene="sopA"
                     /locus_tag="ACHOUK_003020"
     CDS             complement(19507..20673)
                     /gene="sopA"
                     /locus_tag="ACHOUK_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HFP9898417.1"
                     /translation="MKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRIGE
                     AADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRAE
                     DVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPT
                     DPHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQ
                     FFDMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRE
                     TDEVGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
BASE COUNT         4868 a         6210 c         5352 g         4865 t
ORIGIN      
        1 taatttttga ttagcgacaa cctaaaatgt attttgatgt tgttatataa attaatgtat
       61 tcggtggtgc atccaattag tagaacatgt gtttttcgat aaacgctccg atcacttttt
      121 cgtggatctc aactgagcgt gagaagcaga tggttttacg agtcaaccgc ttaatgcggg
      181 tgcgtagcgt cagattatta cgctcaatgc gttgggtgaa tattttgcca gcacgttatc
      241 accgaacatc tctcccccag tcggcgaacc ctttattgga cgtcagcaca atgctcgctt
      301 tttcatatcg acggttcagc aaccggaaga acagactgtg ttcctgcgta aagcgctgct
      361 ccggtgtctt cctgaactgg cgaagcaaca tggaacggcc accggtataa cccatcgctt
      421 tgatttccgc caggataact tcgccgttcc agacattctc agccagacgc atgtcgatat
      481 agtccatttc ggccattttg tggcgtgttt ttctggccgg aggttctggg tatttcaggt
      541 aacgtctgac cgttcgttca gagcaaccaa tctgagtcgc aatatcaaca atatacgcac
      601 cttgctggcg catttgcttt atcatgtaaa agtcctctct gctcagcatg ctgatgtcct
      661 ttctggtgtg agaacctcaa ggaaacaaca tgttgggtgg agcggacaat ccaaatggtg
      721 aattaccgtc ttatatcact ggcggataag gataagtaca cgtcggcagt tccgtcagca
      781 ctgcgacagc gttgtcctcg ttgcttttac ccggtcatgc acaacgcctg atggatgaac
      841 tgcgtgccca gggttaccat tttaaagtaa aaaccgtgac ggaaagcctg cgccgtcacg
      901 gactgagggc aaaagcttcc tggaatttca gtccggtcta ttaccgcgca cacagccagc
      961 ctgtgtcaga aaatctgctg gagcaggatt tttacgccag tggcccgaac cagaagtggg
     1021 cgggagacat cacatattta cgtacggatg aaggctggcc gtatctggct gtggtcacgt
     1081 gccattattg gctgatcaat gtcgtcacgc atgacggcac aggagccccc ggaacgttat
     1141 gctaccgcat gtcatttgca aaaatgacag atagatatac ttcaatttga atcttaacaa
     1201 ttagcatggg aatgttgtca atcctctgta tggagccacc acatattgca atttttgttc
     1261 tcttttaaaa tatattccac gagcgtcttg tgggaccttt tgctgttata ggggggcagt
     1321 ttggatatcg aaaattattg tacgttaagg ctgaatttca atcgcaaagc ataaccttac
     1381 ctttctgctt acttatgcag ttcaaagagt accaactctg caattttcgt tgccacagat
     1441 gcaatatcgt tactttccag tcaccatgcc gctgaaagcc cacaccatgc gataatccac
     1501 atcaagagcc gctttctgtc cagacccgct gttgtgaggt gtgaggtgtg aggtgtgagg
     1561 tgtgaggtag cctgagttta acggacaggc cgatgaagga cgccgttacc gggataatgt
     1621 aagaatcttt ttttgagtat gtcaacataa cctgaggcaa agtacctcag gttccagtaa
     1681 tcagccgacc tgacagtctt ctgttagccc tacagatatg tcgtgtagct aaccgcgcat
     1741 gtcaaagccg gatttttgaa aatctctata aacttccgga ttgttgaagg ccgggaattt
     1801 agctttatgg gctgctgact ttatcgcttc gcaataggct ttatcgccat tactggttga
     1861 tatttttaac gccgtgccat cccgggagaa ttccatatgc aacctgcatt ttttcctttt
     1921 ccatttttgc ggatcatcaa gtttagcatt aattgcatcc ctgattcccc gtgcctgctc
     1981 cccccattca tcctgatcat cccaacgtcc tgaactgcaa ctacccaggg ctgtagtttt
     2041 atggcaattt gaaggatgta atggcgcaca tcccgccaca aagctgacta aaatagccag
     2101 tataacgatt ttctttaccc ccatatattg ctcctcaacc catatagatc tcagtaccat
     2161 agctctgtca gagcaaagaa aatcttaact aatacaagtt aagattttta atatccactc
     2221 ctcatcccta cattccaggt gatggtggcg tccactcatt atcctgaact ggagcataag
     2281 atgcgccatg ccccccggat atgcttttct gttgcctgat atattcgtgg tagtactgtt
     2341 cactacgctg acgatgtaaa tccacatccg ggcggctgat tacaaagtgt ccatttccgg
     2401 ttattccagc tgcattatgc caggccacca tttccgcaaa tacatcatta aactgttgat
     2461 atgagccttc cggttcaaaa cccattcctg gctcacgatc aaacaataca ggagcaatca
     2521 gattcagctt atccaccagt tcatttctgt cctgatgatg gtagaacacc acacggtccc
     2581 cataatccac acagactaac tcgccattcc tccgatcacg aaaacgcaca ctgccatctt
     2641 tctgcagacg ggcttcgaga acgccagtgt cttcataaag tggagttcct cccggttcac
     2701 agagaatcac gcagcggtcg gcattcgttg ttcgtcgctt gtctttgcga cgatcacgat
     2761 aatcccagcc acgtaactgt gataatgcag ctctgtctcc ctgtaccgcc tgttgctcca
     2821 cccactgccg gtaggacaaa ggataccact taccctcctc aatcagtgac aaccgctctt
     2881 ccttcacact ctccttcagc ctgatcagcg cctgcatacg ctgcacttcc gcaatatggt
     2941 aatgcagctt acgcaattgt ggatcccgga actgcacacg gatatacgcc ttgcgccgac
     3001 ggcaggcagc atgaatttcc cgtaaccgtt ctccgtaacg taagtcgggt ttacgccagt
     3061 gctccttcca tgcaagataa cgcgcgcgca aatcttcccg cgcagctgca cgggcttcac
     3121 ggcgttcacg acgcagtaca ggatcgcgcc ggaagccggg ctccacttca tcgctggcag
     3181 caagttccgg attgtagcga cattcaggct tcacccgttc aaatatatct gcacctgcaa
     3241 tctcaaatgg ccctgcctgc ggctcagccc tggataacgt caaatccggg tggatactgc
     3301 tggccttcac tggtgtgagc tcatgattaa acgcatccac aatcacaaga ccatgatgct
     3361 gtttctgcaa cataagccct ttacgcgcaa acatctcatg gcacgcctgc cagctgaaat
     3421 tcccgcccgc gatcatatct tcaatcaacg actcacactg aacagttgtg tacccccaca
     3481 gactttcctt atcagaaaac agttcgcgct ttattgctcc ctgtggacct gtccccatcc
     3541 gcatacgata ccattcgtaa tcactgacag cctgagtacc aagattacgt ccccggaacc
     3601 gttctccggg tataacctga gtaaaaatat ccttcggtac aggctgccat ccaccatcca
     3661 gatcctgcat ataatccagc gtcattaacg gccacacact gtctgcacgt actggtgtct
     3721 gttgtttatc aaatccatca tgcagtacca gatgcccgtg ctggatcccc atccataatc
     3781 cagccgttgc cagagttctg tgcacatcca ggcaactttc cagctcacca tttctcgcca
     3841 tttccggtaa gcgttcggca aaatgacggc aggcgtactg tttcagactc tccgtttctg
     3901 ccactttttc cggccggata tcaacgttta tggcttccgg atcataacga ccgggtgtcc
     3961 ccacctgact gaaaatatct gtcggtacag gctgatattc ccccagtttc cgccccagtt
     4021 tttcagctgt ccataacggt ccgaacgaac tgagtgcaac accttcccgc gctctgtccc
     4081 agccatcctt caccaccagt tccccttcct gcatcgtgat atacagacca tcactggcca
     4141 gccgtttatg caaagataac cagtcctgtg cgggctcttc acgcagcccg gcaatcgaca
     4201 tctgagcaat atattccctg aacgtctgct ttttcccgcg tcgccaggct gttcatgccc
     4261 gtacaatgcc agcaaggaga aaggggccac cggcgaacca gcagcccctt tataaaggcg
     4321 cttcagtgca gtcagaccag catcagccct gaaaaggcgg gcctgcgccc gcctccaggt
     4381 tgctacttac cggattcgta agccatgaaa gccgccacct ccctgtatcc gtctctgtaa
     4441 cgaatctcgc acagcgattt tcgtgtcaga taagtgaata tcaacagtgt gagacacact
     4501 atcaacacac accagacaag agagctgcgt ggtagtttca tggccttctt ctccttgcgc
     4561 aaagcgcggt aagaggctat cctgatgttg tctaagcatg caggggcctc gtgggttaat
     4621 gaaaaattaa ctacggggct tttgtccttc tgccacacaa cacggtaaca aaccaccttc
     4681 acgtcatgag gcagaaagcc tcaagcgccg ggcacatcat agcccatata ccagcacgct
     4741 gaccacattc acttttcctg aaaataaccc gctcattcag gctgtttacg ggcaatccgt
     4801 gtgattgttg ccgcatcacg cacctcccgg agtttgttcg gcaccatcca ccgctccatc
     4861 tcacggacag catgaccggc ctcctttgct gccagactgg cttccgcgat aacctgccag
     4921 aatggcatat cctgccactg ccagcgttcc attccggcca gtgacggaaa acgctcgccc
     4981 cactgccaga acagggtttc caggtcatgc tctgccaccc gatcactcca gcgtgcatac
     5041 cagcttccga ctgtttccgg tgaatggaac gccagttcga tttcagcctg ggcgaccagc
     5101 gcctggtacg cacgggcttt ctgctctgcc tccctcgcct gctggcggct gtacttctgc
     5161 atgaccagcg cggatttatg gcgactgcgt tccctgacac aaaaccgcac cgacgggaac
     5221 aagcgttctg ctgcatcacc gggaagggga aggaggcaga cctcgccctg tgtttcaatc
     5281 agcacatcga tggcatacaa ccactgaaga cggtttccgt gcagttcttc tgcggtcagg
     5341 gacggcatga aaaatcgcag gtcagacagc gatatcctgc ggcttccgtt cagacagcgg
     5401 aaaaacgccc ccgcatacgg atattccgcc agcgtgtttc cctgattgcg gcgtttttcc
     5461 gtctctgcca ctgcccgcca tgcggcctgt tgctctgtgc tgaggggaac cagtgcctgt
     5521 gaacgtgcgc tcatacctga cctccttccg ccatcaggat atggatgatg tcgttaacgc
     5581 tgtatccctg cgtatccagt gacgccgcca gtgccagtga atggctgacg gcttccggtg
     5641 caaaccgccg ctgtacctga accaccgcaa acggctcacc accggcattc accagaacca
     5701 gcacccagac ctgggagaca tcgcccggtg aacataacgc caggtgaaaa cgttcgtggg
     5761 caggcgtgaa acggacctgg acggggaaaa agccgccaaa ctcgctgccg tactcgccgt
     5821 tcatcgtcca gttgtccggc gcatccagct cccgcatcac ggcgtgtgtc agctcacggc
     5881 gtgcatcact tccggcagcc cggatatctt catattcctg tgcgttcatg gtatgtaata
     5941 cattcagagt cagttcagtt ttcatcacgg tatctccctt gctgccgccc cgtctccggc
     6001 ggggcggtgt ggttgttcat caggcggcat cagccaggtt atcggtgcgt tcagtggcat
     6061 ccgtctgtgg gcgtggtgca cacatccagc caggcaccca gcggttgtct ttcatatggt
     6121 gctctgcatg ttcagccgca tcgcctttct tcatcttctc cgcgtcccgt gcggcaccgg
     6181 atagtcctgc ctcattcagg gctgcgataa tctgcggctt tttcaggtgt ccgaaaaagt
     6241 ttgctttcgt cggctgccac cagtcgcgca tgtgaaagcc gatggcgctc tccagcgtgt
     6301 ccagcggact gcgtgacgtg tgaccatact cacgggtctg gacaccgtgg atactgcacg
     6361 cggtgcagaa actgagcagt gataacagca cctcctgact gagtgacagg aacgtcgtca
     6421 tatcccgtgc ccatccatcc ggcagcaggg cggcaagacg ggctttttct gccatcatcg
     6481 ccatgaatgc ggcaccttcc ttccctgacg gcgcatcgct ggtcagcgaa taatgctcac
     6541 attccaggcg gattctggcg ggattgctgt acgctccact gccaaataca ttcagacaga
     6601 gtgtccatgc cagaagtgcc agggatttgt ctgattgctg catcagtgcc gcctggactg
     6661 ccagcgtgcg ctctgaggac attttcgtca gcagcggcag actgatttcc tccactgagg
     6721 ctttctcctg cacctgttcc gtgcggtaat cgttatcagc cacatcatcc tcactgcgca
     6781 actgcacacc acgctggaca cacacgttgc cataacgcca ggacaccacc acgccacttc
     6841 cggcacgcat ctccggcgtc cacgccctga ccttcgccat gcagcgcatc agcttcattt
     6901 cggcttccag caggtcggat tcctcacact ggttttccag cgcatcgtaa cgtgccatca
     6961 gttcgttcag gcgttcttct tctgcctccg tcagcaccgc ttccggctcc ggcagactgc
     7021 ggtatgttcc ggcatcctca cggcagaaac caacaggctc catacgtccg gcgcaccatt
     7081 cccagccttc ggcttcccga aggtgttcgg cgacagcctg gagtttttcc agcagggcgg
     7141 catcgagcgc cacgcagtcc acatagccac cctcgtcgtc actgaacaaa tcggtgcgca
     7201 gttcatctgg cgagaaggta tcagccccca cgaagcggaa tttactgttc cctgccaccg
     7261 ccacttcact ttcggtcacc agacgacgaa tggtctgtac ttccggttta ccgccccatc
     7321 ccgactgaca ggcggcttca aacacctgca cctgacgcgc ggtgtcgttc tccagcgcca
     7381 gcgcctgaca gtgctcggtg gtgatgcggt cttctgccag cgcatcgagg atgacaggcg
     7441 caaggtctgc cagtttcagc attcgctgaa cgtggcgggg cgaataaccc agcaaatcac
     7501 cgatttgtgc aggtgttttg ccttcctgcg ccattgcacg gaatccggca atctgttcgg
     7561 cagggtgcat atcccgacga tgaccgttct cggtcatcga tgcggcagtc gccagctcct
     7621 gcggaatgac cttcacacgg acaggccagt cagccggaat gatgtcacgc tctgccagca
     7681 tgttgagtgc tgccagtcgg cgaccacctg cggcgacacc gtaacggtca cctggcaggg
     7741 catgaacaac cagattctgt aacaggccga cacctttaat ggagtccgcc agttcgctga
     7801 cggactccgc agaatacggc accgtgcgca cattcagcgg tgacttaatc agcgaggcca
     7861 gcggcacgct cacttcctcc gtctgcgcca gcagggccga caggactgtt tcctgcgttt
     7921 ttgcaggttt acggctggat ttacgctccg tttttgcctt agactctgta actgacatag
     7981 ttagtacctc ccaaaacggt aataatgatt gtctgccgga cgcgttcccg tccggcacct
     8041 ctcttactta ataatcccga aatcacggat gacatggttc atccagtacc cggcaccgtc
     8101 ctcaaaattc caggtacgcc agaccattgc accgttatta cggacaacca ggcgaaactg
     8161 ttcgccgtgg tcattctcga taaagacgtt ccggtactgt gcggcaacgg cttcggcatg
     8221 tttgcgggta aaaggaccgt caggcagtcc ctgaagtatt ctgaaatatt cgctcatcct
     8281 catctctccg gtgatgcccc gcacaggacg gggcggttgt cacagtcagt cgttcagaac
     8341 gggatatcgt ctgaaaaccc gtaatcgtca ccctccggcg gttgcggctg aggctgaccg
     8401 ctgaactgtt gcccctcttc cggctgagtc tgagcacctg cggcacgtac cagcatctgc
     8461 atggtgcccg tggtcttaac aagaatttcg gtgacgtaac gggtgatacc gttatcctcc
     8521 cagctacggg tgcgaagctg accttcgatg tagacctgcg cgcccttgcg cagatattca
     8581 cctgccactt ccgcgagctt gccgaacagc accacgcgat gccattctgt ctgctcccgc
     8641 atctcccccg tctgtttgtc acgccagctt tctgacgtgg ccacctgcag gtttgccact
     8701 gcgcccccgt tggggatgta acggacttcc ggatcttttc ccaggcgtcc tacgaggatg
     8761 accttgttaa tgccacgaac tgccatgatg tgtctccttc tgttgatgtt ttcagccccc
     8821 atcatctttt gagtttcggg agccgcggtt ttgacgtttt gcggggacct gccttttgtt
     8881 ccctccggta ttcaccatca cctttttgag aaccttcgcc gcaaggggca tcactgccac
     8941 ggccggaaac gactgtccgg gggcagaccg caaaactttt tgcctgcttt tgggcaaaaa
     9001 gttttgcggg atgtgcattt cacccctgga ctggcggaga cggttgtggc tgtctttgcc
     9061 cccggcgaca ggggcttaaa caggtgaatg gcaccggaca caacaacggg caggcacccg
     9121 ccgcaacgac gtgaagcgcg gtttgcgaaa cggcgttgcc ccttacatcc cggcggcctt
     9181 ttgccgggat gccgggagcg caggccacgg cgcgacagag gcggcgcgac cggaacggtc
     9241 gctgcaagcc cgggtttcgc gtcagggatg gaagcccgcc agggcggaga cagctccgct
     9301 ggctccgttc acgacagccc gccccgccag ggggacgccc ggaaaaggca taagcaggag
     9361 aacaacaaaa cagacagtaa tcagagcagc agaaaaaaga agaacagtag cagtagcagt
     9421 agcagtagca gtagcagtag cagtagcatg aacaacaaca tcatcagtaa cagacaaatg
     9481 acaggtctga aaagacagaa gcccacagca gaatgtccgc gtaatgtaag gggcggcgcg
     9541 tgagcgcggc ccctttcagc gccaccggcg cgcggtcttt tcccggcgcg ggcagacggg
     9601 catttatcct cccgcctgcc cggagtcatc aggcacgcag acgaataatc atcgtcggaa
     9661 catcggtata agcaaacgtg ccgcgtggca gctcctcgcg gacgtcagaa aacggtaaca
     9721 gcttctcctg ctggcgcggc ccgttcagac agacggcgac cagcacgcca cccggacgca
     9781 acagggaaaa ggcccgcagg atatgccgga tatcctgccc gtggctgaac ggcggattaa
     9841 tgataacccg gctgtaatac tgcaccggct gccattccat gaagtcgcca cactgaaccc
     9901 tgacaccgtt aaaattttcc cgcagatacc ggaccagccc gctgttgatt tccaccgcat
     9961 cacacatggc ctccggtgcg gtatcccgaa tcgcccgcag tatggccccg gtgccggcag
    10021 agggttccag aatgtgatca cggttgctga tctccgccag cgtcaccagg cgatcacaga
    10081 ccggggcagg agtcacaaaa agctgagatt cagcccggat ttcagcaacc ggcgcggcgt
    10141 tatccagcct tgcgcgcatc agccggatac gttcttttaa ctgactgtgc atgtcattat
    10201 ctccgtcatc ccccggggat ttcccggggg gatatacctt atttctgtgg gatttccgta
    10261 attttcatgt cggtgatata cacattcacc agacggaaat tattgtccat tgtgcggcgg
    10321 tagcggtatg ccccgtggtc ttctgcttct gccacactgc gcacggcctt acagtcccgg
    10381 ggcagggcgg cccactgtgc ctttgtcatt tcccggaaac cttcccccgg atagttgact
    10441 accggcggac gcttcgcggc ctggctggcg acggcagcct cttctgccga tggtgctttg
    10501 tagtccgtga tgcgatcggg cgtcactttc atcgtgccgc tgtacccgag aaaactgtaa
    10561 ttcggcgttg tgactgaact caccgccccg ttgcttttgt tcacgcggat gatggtcagc
    10621 cactcgcccc ggctgaaaac ctgtccgccc ggctcaaaat cctgtgtccg ggtaaccacg
    10681 ccgccgcttt cgtccagcat ggcacgctca tagttcaggc ggttctgata gtggttaacc
    10741 cagcgttgct gatgctgaat ctgccgttca tgacagcgga tcgcaatttc gcgggcctgc
    10801 tccgtggtga tgatgtcatc atccagggcc gaccataacg accggctccc ttcatactgg
    10861 cttttttctg ccgggcgcgg atacgtatcc agcgggaaac aggcgctgat atggtcatga
    10921 ctgctgatga gttttgccat attcagatcc agcgattcag cccgccacat cgtcagatat
    10981 ttctgcgact gcgcgatggt cttttcagcc ttgcgcaaat cagcttcgat ttttttgata
    11041 cgacgccagc gaacgtccgg acgttcttta tacttcgcgt gaagcagtgc tgaccgcccc
    11101 cgttcttccc agtattccgc acgttcaaag agcatcacgg cacgtttcat gccgttttca
    11161 atacgctgcg catcacgacg ggcgcggcgt tcgctgtgat gccccacaag aatgggctga
    11221 ccgggcggga tcatcgcggc cagtctttcc acttcatcaa gtgcctgtgc ggattcactg
    11281 gcccgctttc cgctgtatcc ggtaaaccgc tccgcccgtg cttcctgacg ttcagcgagc
    11341 gtgctgtctt catcctcaat ctctccggca agtgagagga gcacgtcttc ccggcccggt
    11401 gtccaggccg gcgcgacaaa cagcgcctgt tttggtgccc agcgaaaacc ggcatcatgc
    11461 acttttttat acgtctccgg gtcaagacgt gacacggcat acaggcgcag tttgttgtca
    11521 tccggtgagt aggtcgcgcg gtacatctgc acagggctgg cggcttccgg ggcggtcatg
    11581 gtgattgctg ctgatgtcat aaaatgttct ccttccgtct gttgccgccc ctcgcggggc
    11641 ggctcacctc atcagttaaa agcgcgtttc agttcgagtg tgcggagcac ctgcccgaaa
    11701 gccgtgtttt tcatggcggg gatgtgtaca ccacgccccc ggtgaaaact ccgggctatg
    11761 tgtcgcccga aggctttcag cacggggtca atttccggag cggggatgtc ttcgcgtctg
    11821 aaacggggat gacggcgggg ctttacagcc gctgcggaag gtgaaacaat gcttacagtc
    11881 ataaatggat tccttatgac aaattggtta acaatgccgt caaaattggt cgcacaattc
    11941 ttacggcgtg gattaagcag gtccctgacc tgcttttttc atatctgcat ctgacgcttt
    12001 ccggtgtttt cctccttctg ttcctgcgtt ctcattacct gtcagccgga tgactgacag
    12061 aacacataaa tgcgtattgc cggagcctga aataaatcct gatttttaaa gaacatcacc
    12121 cgttgagttt cgggagccgc ggttttgacg tttcgcggga acctgctgct gtattccctg
    12181 tggtatgaac ttcacccgtt tgagaacctt cgccgcaagg ggcatcactg ccacggcctg
    12241 acacacctgt cctgggcaga acgcaaaatt ttttaccggg ttctgggtaa aaaattttgt
    12301 gggctgttca tttaccctgg acagtcagtg cggcaggttg tggctgtatc agctcccggc
    12361 gacagggact catacgggtg tgtggcaccc ggcaggcaat gagcagggac ccgccgcaac
    12421 gacgtgaagc gcggtttgcg aaacggcgtt gccccttaca tcccggcggc cttttgccgg
    12481 gatgccggga gcgcaggcca cggcgcgaca gaggctgcgc gaccggaacg gtcgctgcaa
    12541 gccagggttt cgcgtcaggg atggaagccc gccagggcgg agacagcttc gctggctccg
    12601 ttcacgacag cccgccccgc cagggggacg cccggaaaag ccataagcag gagaggaacg
    12661 gaaagaagag gaggaagagg aagaggaaaa agaagaagaa cagtaacagg cacggtaacc
    12721 acagaaaaat aacatcagaa aaaaacattc cggacacatc acaaacagga agggtaacgg
    12781 acgacgcgtc agcgtggccc gcttcccttc cggcaggcgt cagcctgcca ggggttttcg
    12841 cgcgggctga caggcggcct ccaccgccag ccctgtatca cctgtttctg ctgccctgta
    12901 agatgtagtg accgcccagg aaaacagcct gctgattcgc cacaaaatcg cagatttcat
    12961 caacaatctg cgcggtggtg agcggtttct gccccatttc acgacgtcgc gcattaatca
    13021 cggctttgta attgcgcaga atttcgcggg tttctgtgct gatattcatg acatgtgctc
    13081 cccgtccagc gcctgtaaca caacgtccat tgcttcgcgt tcctccctga cgaacgcatc
    13141 accgtcgccc ccgttctcac tcccgacggc atacaggcgc ttcatttcac tgcctgccag
    13201 cgccatcact ttttccagca tcaccgccgg aactgtcacc tgacggctgg cacgcttttc
    13261 ttccatcaac tcacgaacaa cgtcgtgaac aatgctgaca tccgcaccat attcaaaggc
    13321 atcgtccagc cgctgcatga cggtttccag ctcatcgtct gacaggttcc agtcctgggc
    13381 tatgtggcgc acatccccac gggtcagcac cacggcaaaa acgcattcac tgtccggcac
    13441 gtctgtatcc agtacatcgc ggatgatttc tttaacagta cagtacatgt tctcgtctcc
    13501 tttacagggg cggcattgcc gccccgtccg ttaatcagtc cagtgccgcg taaatcgcgt
    13561 tgcattcagg gtgaaattcg atgtgacgcc acaactgcgc atcgcgcatc cggtaaagct
    13621 gggtcagtcc tgcatcaccg ctgtcgtggt acagccacaa ctggcggtta atcacaaggg
    13681 aagtcagaat gatgccgcag gcatcagcac tcacggtacg gtcaaaccag tttgcaccgt
    13741 tcaccatgtg gaaacggtcg ccatccggca tcatgtaacc gccaccgtcc ggcaggcgga
    13801 tgaagtgcca gaagcctccc tgatattccg gcatcatttt ttgcgccagc gcgtacacat
    13861 acgcctcggc atacagaaaa tcgttgccaa acaggtaagg cagaaaggag agacggtcgc
    13921 actcttcaac attcagagtg acaggtttcg catattgcat gatgttttcc tccgttgttc
    13981 acatattccg cgacaccggg cggtgccgtg cctccagaaa agccgggcga taaggcgggg
    14041 atgcacaggg cgcaagggaa gccgcagcct gacgcacacg gctgacggga gttgcagggt
    14101 gaggccgcga aacctgagcg ggcgaacgac cggaaaatca cggcagacgg gaaggaggga
    14161 ccgggtgacc gcagcggcga agcgtaccct tgcatcaacg ccagcccggc taagatggag
    14221 gcacggcacc gcccgcacac gacgctgagg ccacataact gaaaaaccac aaggccatgc
    14281 cctgcatggc cgggtaaagc ggagccagcg gcgacttcct ttgtgctctg tccggggacc
    14341 ggcaggaaaa aggcggggat ttaccccgcc aacgtcatca tttccggatt tttgccaggg
    14401 catccgccat gctgcggatt tcgttccggg cttcccggac cacctcccgc agaacaccac
    14461 ggtcaaacca ccggcggggc ggttcatccg gtatacgcag cacatcgcag gccagcccac
    14521 tggacaggat atgttcaata caatcgctga ccagatacat ttccaccgcc gaaaaacgtg
    14581 cgccccggtt caggatggca tcgagcacca cgggcaggtt ttcgtcgtcc tcatcgagcc
    14641 agcaggggtg atcgctttcc ttcacgacca ggcgaaagcg gcgcgtctgg cgggtcagta
    14701 acgtctgacc ggtggtggtc agcgccgccc ggatacgccg cgtatcttct gccacccggc
    14761 aactgacact gcgtaccgca tcgcgcaccg ccgcaagtac agccctgatt tcgtcttcgg
    14821 tcagccattc accggcatac ggaaaagaca cctgcgttcc gtctgccgga agcaccggca
    14881 gcgactgcca ctgactgccg gacagaacaa gttccgctcc ggttccggtc agtttctgaa
    14941 tggcggaggc caggtgcggc ggcagtacgc cgccgtggcg gttaacgagt aacgtcgcca
    15001 gccggggcgc atccagcaga acataaatgc tgatccagcc cggatacttc gggttacgct
    15061 ggtcgacatc cgtctcttcc ggccagccct gcgccagcag caggttacgg gcgtgacgac
    15121 aaatcagtgc gttcagcgtt gtgttttcgt tcatgattaa tcgttctccc tgtatcgccg
    15181 cccgcaggcg gcgataacat cagccgtttt cgtgtgtgtt gtttttcagc aggatgcggg
    15241 cctgattaac caccgcctga cggcgggtaa cgcattccgg gacggcgaga ccgtaatcac
    15301 gggcggccca ttcacgtttc cacagtgcct gttcggcggt ctgaatcagg gacgccagca
    15361 gttcagccgg gacggtcacc tggcggtttt cgctcacgtt gctgataatc tccatcgccg
    15421 cggcagaaga aataccggac tcaattcgct ggtcttccgg gatgtcctcc aggcgcgcca
    15481 ggacggtcct gatttcagga tcggacaggg aaatatccat tccgtcagcg agggcctgaa
    15541 tctcttccgg ggaccagata accagcatca gcggtgtata tcccggatac tttgctgcca
    15601 gctcccggca tagcgtttcg catgttccgt acattctcgt ctccttctca atctttaaag
    15661 cacgcatcca gttccagaat ttcggtatac gcctccatga acaggcgatt ggcgcgatac
    15721 agcgtctggc gtgccaggcg ttcaacaagc acatcggaat caaagcgaca gcgatgctcc
    15781 atatcgttca gcctggcccg aaggtcacac atggtgttgg ccacctccgc cacgtcggcg
    15841 cgaagttttt catgtccggc atagttcatt tacgcagcct ccggcataaa ccagtcatca
    15901 tttgcggccc cctgctgcat ggcccgctgt acggcagcca gacgttgctg tccggcacgg
    15961 tgatactgtt caagcagctc gataccgata taccggcgtc cggactgaag ggcggccaca
    16021 caggttgagc cgctgcctgc aaacgggtcc agcacaatcg cgttcgggtg tgtgaagctc
    16081 tcaatcagtg gttgcaggct ggtaacaggc ttttccgtcg ggtgatgacg attgcctgaa
    16141 tatttccagc ccagcacgtc gggcaatggc ttctgaggca gcgccggacg cccttttgcc
    16201 aggatgtaag cgcattcgtg gcgatagccc acatatgcgg ccttcgaagt gtaggttttg
    16261 gtaaacacca ggtggccaac cacgctgaat cccgcatttt tccaggcggc cataaagcga
    16321 tcgacgcggt tccagccata gaagctcacc atcagcgcat cttttttcag cacgcggtac
    16381 atttcattac aggccggttg cagccattca tcggttttat cgccggcgat ggtacgcccc
    16441 tgacggtcac ggaaaccgac gagatatggc gggtcggtga ggatgaaatc gacaccattg
    16501 cccggaatcg tggccataac acgcacgcag tcaccctgga taaaacggga cataacagca
    16561 ctccttctct tacagagggc accgggaggg cgcccctgtg gcgcccgttc cccgcggcct
    16621 gtgggctgtt caccgcgtcc ggtaacgggt caggccgacc gtggaatatc gcagccgtcg
    16681 tctttttgac ggcgagaata tgccgcgaaa gcggcttgaa gggcgttcat cagaaaaggg
    16741 cgacgggctg gcgtacactg tcaccgatgc cagccgggag cggtaaaagc gcccgtcccg
    16801 tgcccggccg gacgcgggac actgaccaca ggcagcgttc ctgccgcgcc gcgtcagccg
    16861 gcgcagtaaa aagaggagcc cggcgacatc ctttaccgga aaagaaacgg gcggttgccc
    16921 gcccgtctcc gtcatacttc atccaccgta atgcgccacc ttgtcgacta tccactccgg
    16981 cgcggtgact tccggcagct catcatcatc tgtcggggaa gaccattcca gttcccccca
    17041 cagcacatca acaagctctc cacgctcata tcgctgccag ccacagaaat tgcagccctg
    17101 cccggcgtac cagtgctcca gcatgcaact gaaacggcgg cttaatactg caataacgtc
    17161 gctttccggc tgacaccacg gcgtgtcgaa atcaacctga atgaagttgt ctccctgact
    17221 gctgatattc acctcatacc cacaaggcca cttcacgcca tactgttccg tataccagtg
    17281 atatgcagaa ggaacaccgt tcagaacacc accgttaaag ccattcactt caacatccag
    17341 gcgagtggac agaaccgtca gcaggtcaaa cggcatggtt ttttccggca ggacgttgtc
    17401 acacagacgg ttccaccaca cagatacatc ctcgttactc cagatgtcgc accagtcacc
    17461 tctttttggg gtgaaaagag cggtaatgct ttccctggca tcatccggta attcttccca
    17521 gcgacgccgg ccagtaccgc tctgcagcca gagttcatgc agcatcaggc agttttgctc
    17581 atccagaagt acaccgtcct gcaggtgtgt cagccagcgg gtgaacgcga tattctccgg
    17641 cgatacaacg ccacgcccgg cagccgtcag tccggggcat ggttcaaacc gcacatcttc
    17701 agtggtctgc agaagtccgg cacttccggc cagaaacagc tgaatacctt catttgtggc
    17761 gcggcgataa agcggtgtga ctgcaccgct ggccagtcgt ttaatctcag cgatctgtgc
    17821 cggttcacca gaaaaataca tacgattcga gcaccagttt ggcatgggaa ttctcctctc
    17881 acatgcggcc tccatcaggc cgtgccgcac ctgtaaccgg gcgaccgcat acgaatgcag
    17941 gtgcggcccc gaagccgcag cgcgtggagg aggaacgacg aaacgtgacc gggtgaggag
    18001 gccggcgaca gcgacacact tgcatcggat gcagcccggt taacgtgccg gcacggcctg
    18061 ggtaaccagg tattttgtcc acataaccgt gcgcaaaatg ttgtggataa gcaggacaca
    18121 gcagcaatcc acagcaggca tacaaccgca caccgaggtt actccgttct acaggttacg
    18181 acgacatgtc aatacttgcc cttgacaggc attgatagaa tcgtagtctc acgctgatag
    18241 tctgatcgac aatacaagtg ggaccgtggt cccagaccga taatcagacc gacaacacga
    18301 gtgggaccgt ggtcccagac cgataatcag accgacaaca cgagtgggac cgtggtccca
    18361 gactaataat cagaccgacg atacgagtgg gaccgtggtc ccagtctgat tatcagaccg
    18421 acgatacaag tggaacagtg ggcccagaga gaatattcag gccagttatg ctttctggcc
    18481 tgtaacaaag gacattaagt aaagacagat aaacgtagac taaaacgtgg tcgcatcagg
    18541 gtgctggctt ttcaagttcc ttaagaatgg cctcaatttt ctctatacac tcagttggaa
    18601 cacgagacct gtccaggtta agcaccattt tatcgccctt atacaatact gtcgctccag
    18661 gagcaaactg atgtcgtgag cttaaactag ttcttgatgc agatgacgtt ttaagcacag
    18721 aagttaaaag agtgataact tcttcagctt caaatatcac cccagctttt ttctgctcat
    18781 gaaggttaga tgcctgctgc ttaagtaatt cctctttatc tgtaaaggct ttttgaagtg
    18841 catcacctga ccgggcagat agttcaccgg ggtgagaaaa aagagcaaca actgatttag
    18901 gcaatttggc tgtgttgata cagcgggtaa taatcttacg tgaaatattt tccgcatcag
    18961 ccagcgcaga aatatttcca gcaaattcat tctgcaatcg gcttgcataa cgctgaccac
    19021 gttcataagc acttgttggg cgataatcgt tacccaatct ggataatgca gccatctgct
    19081 catcatccag ctcgccaacc agaacacgat aatcactttc ggtaagtgca gcagctttac
    19141 gacggcgact cccatcggca atttctatga caccagatac tcttcgaccg aacgccggtg
    19201 tctgttgacc agtcagtaga aaagaaggga tgagatcatc cagtgcgtcc tcagtaagca
    19261 gctcctggtc acgttcatta cctgaccata cccgagaggt cttctcaaca ctatcacccc
    19321 ggagcacttc aagagtaaac ttcacatccc gaccacatac aggcaaagta atggcattac
    19381 cgcgagccat tactcctacg cgcgcaatta acgaatccac catcggggca gctggtgtcg
    19441 ataacgaagt atcttcaacc ggttgagtat tgagcgtatg ttttggaata acaggcgcac
    19501 gcttcattat ctaatctccc agcgtggttt aatcagacga tcgaaaattt cattgcagac
    19561 aggttcccaa atagaaagag catttctcca ggcaccagtt gaagagcgtt gatcaatggc
    19621 ctgttcaaaa acagttctca tccggatctg acctttacca acttcatccg tttcacgtac
    19681 aacatttttt agaaccatgc ttccccaggc atcccgaatt tgctcctcca tccacgggga
    19741 ctgagagcca ttactattgc tgtatttggt aagcaaaata cgtacatcag gctcgaaccc
    19801 tttaagatca acgttcttga gcagatcacg aagcatatcg aaaaactgca gtgcggaggt
    19861 gtagtcaaac aactcagcag gagtgggaac aatcagcaca tcagcagcac atacgacatt
    19921 aatcgtgccg atacccaggt taggcgcgct gtcaataact atgacatcat agtcatgagc
    19981 aacagtttca atggccagtc ggagcatcag gtgtggatcg gtgggcagtt taccttcatc
    20041 aaatttgccc attaactcag tttcaatacg gtgcagagcc agacaggaag gaataatgtc
    20101 aagccccggc cagcaagtgg gctttattgc ataagtgaca tcgtcctttt ccccaagata
    20161 gaaaggcagg agagtgtctt ctgcatgaat atgaagatct ggtacccatc cgtgatacat
    20221 tgaggctgtt ccctgggggt cgttaccttc cacgagcaaa acacgtagcc ccttcagagc
    20281 cagatcctga gcaagatgaa cagaaactga ggttttgtaa acgccacctt tatgggcagc
    20341 aaccccgatc accggtggaa atacgtcttc agcacgtcgc aatcgcgtac caaacacatc
    20401 acgcatatga ttaatttgtt caattgtata accaacacgt tgctcaaccc gtcctcgaat
    20461 ttccatatcc gggtgcggta gtcgccctgc tttctcggca tctctgatag cctgagaaga
    20521 aaccccaact aaatccgctg cttcacctat tctccagcgc cgggttattt tcctcgcttc
    20581 cgggctgtca tcattaaact gtgcaatggc gatagccttc gtcatttcat gaccagcgtt
    20641 tatgcactgg ttaagtgttt ccatgagttt cattctgaac atcctttaat cattgctttg
    20701 cattttttta ttaaatcttg caatttactg caaagcaaca acaaaatcgc aaagtcatca
    20761 aaaaaccgca aagttgttta aaataagagc aacactacaa aaggagataa gaagagcaca
    20821 tacctcagtc acttattatc actagcgctc gccgcagccg tgtaaccgag catagcgagc
    20881 gaactggcga ggaagcaaag aagaactgtt ctgtcagata gctcttacgc tcagcgcaag
    20941 aagaaatatc caccgtggga aaaactccag gtagaggtac acacgcggat agccaattca
    21001 gagtaataaa ctgtgataat caaccctcat caatgatgac gaactaaccc ccgatatcag
    21061 gtcacatgac gaagggaaag agaaggaaat caactgtgac aaactgccct caaatttggc
    21121 ttccttaaaa attacagttc aaaaagtatg agaaaatcca tgcaggctga aggaaacagc
    21181 aaaactgtga caaattaccc tcagtaggtc agaacaaatg tgacgaacca ccctcaaatc
    21241 tgtgacagat aaccctcaga ctatcctgtc gtcatggaag tgaaccgccc cgggt
//
LOCUS       DAZKIP010000074        20445 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZKIP010000074 DAZKIP010000000
VERSION     DAZKIP010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.074"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(210..956)
                     /gene="cutC"
                     /locus_tag="ACHOUK_003021"
     CDS             complement(210..956)
                     /gene="cutC"
                     /locus_tag="ACHOUK_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFP9898418.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MSRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(970..1536)
                     /gene="yecM"
                     /locus_tag="ACHOUK_003022"
     CDS             complement(970..1536)
                     /gene="yecM"
                     /locus_tag="ACHOUK_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9898419.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPR
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1752..3485
                     /gene="argS"
                     /locus_tag="ACHOUK_003023"
     CDS             1752..3485
                     /gene="argS"
                     /locus_tag="ACHOUK_003023"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFP9898420.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTHDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEIDEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3527..3571)
                     /locus_tag="ACHOUK_003024"
     CDS             complement(3527..3571)
                     /locus_tag="ACHOUK_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9898421.1"
                     /translation="MPIFRIHLDGNKKA"
     gene            3662..4150
                     /gene="yecT"
                     /locus_tag="ACHOUK_003025"
     CDS             3662..4150
                     /gene="yecT"
                     /locus_tag="ACHOUK_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFP9898422.1"
                     /translation="MFKFLVLTLGIISCQVYAEDTLIVNDHDISAIKDCWQKNSGDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKPDTEQLNLLKKSQRTWL
                     DFRDKECELILSNEDVQDLSNPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4270..4662)
                     /gene="flhe"
                     /locus_tag="ACHOUK_003026"
     CDS             complement(4270..4662)
                     /gene="flhe"
                     /locus_tag="ACHOUK_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFP9898423.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELDGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPSLKVQRNEVIVNYR"
     gene            complement(4662..6740)
                     /gene="flhA"
                     /locus_tag="ACHOUK_003027"
     CDS             complement(4662..6740)
                     /gene="flhA"
                     /locus_tag="ACHOUK_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFP9898424.1"
                     /translation="MSNLAAMLRLPTNLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNAVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6733..7881)
                     /gene="flhB"
                     /locus_tag="ACHOUK_003028"
     CDS             complement(6733..7881)
                     /gene="flhB"
                     /locus_tag="ACHOUK_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFP9898425.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIIKDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPHMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHVEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(8083..8727)
                     /gene="cheZ"
                     /locus_tag="ACHOUK_003029"
     CDS             complement(8083..8727)
                     /gene="cheZ"
                     /locus_tag="ACHOUK_003029"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFP9898426.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRKFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8738..9127)
                     /gene="cheY"
                     /locus_tag="ACHOUK_003030"
     CDS             complement(8738..9127)
                     /gene="cheY"
                     /locus_tag="ACHOUK_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFP9898427.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(9142..10191)
                     /gene="cheB"
                     /locus_tag="ACHOUK_003031"
     CDS             complement(9142..10191)
                     /gene="cheB"
                     /locus_tag="ACHOUK_003031"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFP9898428.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLNQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10194..11054)
                     /gene="cheR"
                     /locus_tag="ACHOUK_003032"
     CDS             complement(10194..11054)
                     /gene="cheR"
                     /locus_tag="ACHOUK_003032"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFP9898429.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFASLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(11073..12674)
                     /gene="tap"
                     /locus_tag="ACHOUK_003033"
     CDS             complement(11073..12674)
                     /gene="tap"
                     /locus_tag="ACHOUK_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFP9898430.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTTLNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEIIHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(12720..14381)
                     /gene="tar"
                     /locus_tag="ACHOUK_003034"
     CDS             complement(12720..14381)
                     /gene="tar"
                     /locus_tag="ACHOUK_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFP9898431.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVDAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDHVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(14524..15027)
                     /gene="cheW"
                     /locus_tag="ACHOUK_003035"
     CDS             complement(14524..15027)
                     /gene="cheW"
                     /locus_tag="ACHOUK_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFP9898432.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(15048..17012)
                     /gene="cheA"
                     /locus_tag="ACHOUK_003036"
     CDS             complement(15048..17012)
                     /gene="cheA"
                     /locus_tag="ACHOUK_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFP9898433.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKASEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(17017..17943)
                     /gene="motB"
                     /locus_tag="ACHOUK_003037"
     CDS             complement(17017..17943)
                     /gene="motB"
                     /locus_tag="ACHOUK_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFP9898434.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVCVPTMPSAEPR"
     gene            complement(17940..18827)
                     /gene="motA"
                     /locus_tag="ACHOUK_003038"
     CDS             complement(17940..18827)
                     /gene="motA"
                     /locus_tag="ACHOUK_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFP9898435.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRIIADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(18954..19532)
                     /gene="flhC"
                     /locus_tag="ACHOUK_003039"
     CDS             complement(18954..19532)
                     /gene="flhC"
                     /locus_tag="ACHOUK_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFP9898436.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(19535..19885)
                     /gene="flhD"
                     /locus_tag="ACHOUK_003040"
     CDS             complement(19535..19885)
                     /gene="flhD"
                     /locus_tag="ACHOUK_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFP9898437.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITLLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4950 a         5596 c         4945 g         4954 t
ORIGIN      
        1 ttgtcagttt tagcaattta aatcacataa ttaatgtgaa tatataaaat atttttagca
       61 caacgctatg tgcactgtca cccgctcaac agatcgcggt aatcgttcat tttttatact
      121 attcagggat aaaaaaaggc ccctgttgaa attgcagggg cctggtacga gcaagcatca
      181 tattgggcga catgatgcaa cggtaaaaat catttggcct gatggcgttc aatgattcct
      241 ttcatttcag caaccgccgc cccgtctacg acataacgcg aatactcgtc cgcgtgtgca
      301 tctgatgaca tggacaatcc ttgattacga taacgcatcg gtgaggcttg ccacgctccc
      361 gcggagctat ggacttccag cactccggca tcgaggaagt ggtgcaggtt ttctgcacgg
      421 actcctgctc cggccataat gattggagca tcacgatggg caataagttc cataattttt
      481 gataaacctt gcagtgcgtc tgatttttgc cctgatgtca gtacccgggc aatgcccaat
      541 tccgcaagat tattgagtgt atttagaggg ttagcgcaca tatcaaaggc gcgatgaaaa
      601 gtcactgcca gcggaccggc agcagccatt attttttcca ttcgtgacat atcgacatta
      661 ccgtcaacat cgagcacgcc cgtcacgagt ccaggaaaac ctaattcgcg gaccgtgcgc
      721 acatcctcaa gaatggcggc aaactcaccg tcgctgtagc aaaaatcacc accgcgtggg
      781 cgaattatcg gatgcacagg gatcgtcacc cgctggcgca cggatttcag tacacccaac
      841 gacggcgtta agcccccctc ttttggggct gcgcataatt caactctgtc tgcgccgttt
      901 tgctgcgccg ttagcgcgca ttccatgctg taacagcaaa tttccagtaa tgccattttt
      961 gctccttaat tacgccgact gctcgctggc aacaatctct tcaatggacc acggatgaaa
     1021 tttaatggtc gttttgccat cggtgaccgc cagcgtcgga ttcggtaatc gttcatgctc
     1081 accttttggc gaactggttt taactgagat ccccggtagg ctgagtcctt catcagaaag
     1141 caatgccagc gcacgggcat tcagcgtttc tggatcaccc ggcaaaacga tttcaatatg
     1201 ttcccaacct tcgtgcgggt aacgtttttc cctaggccac ggtagctcca caatagaaaa
     1261 ctgccagtgt gcaacctgta ccggttcatg caatttaaac agacaaatcg gtctgccatt
     1321 gatcatattt tctgacaaaa gctcgccaca ctgttcaaac ccgcgacgcc agcgctcagc
     1381 agtggcgttt tgatggcaac gcaaagaaat gtgatcggca gtcagcggag tgatattcaa
     1441 ccccagacgg cgggaaagtt catctaatgc gtgggtaaat cgcggtaaat ccgatgcaat
     1501 atcctgcagc tcgtcgatag attgccagtt cgccataatc actcttcgtc tttcagtaaa
     1561 agcgttaatt taccctgttg ccctgcgcca accaaccgct gatttcacgc cgcttctgat
     1621 gcaatagtga aaacggcaat acgccacgcg cacgttgctg acgaaaacag ccatttgcag
     1681 tatactcccg ccctaatttc tttaactggt gcgggcaatt tttgctcgct tcatcaatgt
     1741 aaggtattcc ggtgaatatt caggctcttc tctcagaaaa agtccgtcag gccatgattg
     1801 cggcaggcgc gcctgcggat tgcgaaccgc aggttcgtca gtcagcaaaa gttcagttcg
     1861 gcgactatca ggctaacggc atgatggcag ttgctaaaaa actgggtatg gcaccgcgac
     1921 aattagcaga gcaggtgctg actcatctgg atcttaacgg tatcgccagc aaagttgaga
     1981 tcgccggtcc aggctttatc aacattttcc ttgatccggc attcctggct gaacatgttc
     2041 agcaggcgct ggcgtccgat cgtctcggtg ttgctacgcc agaaaaacag accattgtgg
     2101 ttgactactc tgcgccaaac gtggcgaaag agatgcacgt cggtcacctg cgctctacca
     2161 ttattggtga cgcagcagtg cgtactctgg agttcctcgg tcacaaagtg attcgcgcaa
     2221 accacgtcgg cgactggggc actcagttcg gtatgctgat tgcatggctg gaaaagcagc
     2281 agcaggaaaa cgccggtgaa atggagctgg ctgaccttga aggtttctac cgcgatgcga
     2341 aaaagcatta cgacgaagat gaagagtttg ccgagcgtgc acgtaactac gtggtaaaac
     2401 tgcaaagcgg tgacgaatat ttccgcgaga tgtggcgcaa actggtcgac atcaccatga
     2461 cgcagaacca gatcacttac gatcgtctca acgtgacgct gacccatgat gacgtgatgg
     2521 gcgaaagcct ctacaaccca atgctgccag gaattgtggc ggatctcaaa gccaaaggtc
     2581 tggcagtaga aagcgaaggg gcgaccgtcg tattccttga tgagtttaaa aacaaggaag
     2641 gcgaaccgat gggcgtgatc attcagaaga aagatggcgg ctatctctac accaccactg
     2701 atatcgcctg tgcgaaatat cgttatgaaa cactgcatgc cgatcgcgtg ctgtattaca
     2761 tcgactcccg tcagcatcaa cacctgatgc aggcatgggc tatcgtccgt aaagcaggct
     2821 atgtaccgga atccgtaccg ctggaacacc acatgttcgg catgatgctg ggtaaagacg
     2881 gcaaaccgtt caaaacccgc gcgggtggta cagtgaaact ggccgatctg ctggatgaag
     2941 ccctggaacg tgcacgccgt ctggtggcag aaaagaaccc ggatatgcca gccgacgagc
     3001 tggaaaaact ggctaacgcg gttggtattg gtgcggtgaa atatgcggat ctctccaaaa
     3061 accgcaccac ggactacatc ttcgactggg acaacatgct ggcgtttgag ggtaataccg
     3121 cgccatacat gcagtatgca tacacgcgtg tattgtccgt gttccgtaaa gcagaaattg
     3181 acgaagagca actggctgca gctccggtta tcatccgtga agatcgtgaa gcgcaactgg
     3241 cagctcgcct gctgcagttt gaagaaaccc tcaccgtggt tgcccgtgaa ggcacgccgc
     3301 atgtaatgtg tgcttacctg tacgatctgg ccggtctgtt ctctggcttc tacgagcact
     3361 gcccgatcct cagcgcagaa aacgaagaag tgcgtaacag ccgtctgaaa ctggcacaac
     3421 tgacagcgaa gacgctgaag ctgggtctgg atacgctggg tattgagact gtagagcgta
     3481 tgtaatcgat ttttcgtgag agtgaagcct gatcaggttt agccgatcag gcttttttat
     3541 taccatctaa atgtattctg aaaataggca tgccattgtt ttctcgctgt tgagtaagag
     3601 gccagaagcg taatatccag ctccagggaa acggtaacgg ttcaatttaa ggaataccgc
     3661 agtgtttaaa tttcttgtat taacattagg cattatctct tgccaggttt acgcagaaga
     3721 tacgcttata gtaaatgatc atgacatttc agccatcaaa gattgttggc aaaaaaattc
     3781 aggtgatgat actgacatta acgtgatcaa atcatgtctg cgacaagaat acaatctcgt
     3841 cgatgcgcaa ttaaataaag cttatggtga agcttatcgt tatatagaac aagtgccacg
     3901 cacaggtgca aaaaaacctg atactgaaca acttaacttg cttaaaaaat cacaacgaac
     3961 ctggctggat tttagggaca aagaatgtga attaatcctt tcaaatgagg acgttcagga
     4021 tttaagtaac ccttattctg aatcagaatg gctatcatgt atgatcatac agaccaatac
     4081 gcgaactcgc cagttgcagc tataccgtaa ctctgaagat ttttatccaa gccctttgac
     4141 aagaggataa ttcacatatt tttggcatgt tttgttgcaa gctattcctg ataaatgatt
     4201 gcaacaaaac atccagcctt tttcactgag ttattaaaca tactcgcgag cgcgtaattt
     4261 ttttgtcctt cagcgataat tcacaatcac ttcattacgt tgtactttca gcgacggaat
     4321 taaccgccca ccgccaggca cttcccagat aaagcgcaac ggttctgctg ccgctatacc
     4381 ggaaaaggcc acggtggttc cgctctgccc atctaattcg acacaacgag attgcgaaca
     4441 caagcgaacc cgcagtcctg caggtgtcgg gccgataagc tgataccgcc acgccaccag
     4501 cgtcatcaat cctgaagcag gttgtcgcgt agaaagaggt gcagacgaca tcgactcacc
     4561 gcgatgattt agcgtaattc cgacactact tgcctgccac atcccctccc cggcggcttg
     4621 caccagcagc ggaaacaata atattgctaa taaggttctc atcatttacc gccaattgtc
     4681 gccgtcatgc ggatatgtcg gttatcagac agttccagat tcgacagcac cactaactgc
     4741 ggcaagctgc ggcgcagaaa gcgagacaat aatggtcgca gcgcgtggtt caccaataaa
     4801 actggcggcg cacccaacat ctcctgacgg gatagcgctt cctgagtttg cgccagtaaa
     4861 cgatccgcca gccctggctc cagtccgccc ccgccctgca acgcctgtag caacaaacgt
     4921 tccagcggtg tatcgaggcc aataacatgg acttcatctt tgccaggaaa ccactgctgg
     4981 gtaatcgccc gtcccaacgc cacgcgcacg acggcggtta attcatgtgg atcgctttgg
     5041 atgggcgcat gttccgccag cgtttcgaga atagtgcgca tatcgcgaat cggtactttt
     5101 tcatcgagga gattttgcag cactttatgc agtgtggtga gcgtgacgac gccagggacg
     5161 agatcttccg tcagctttgg catctcctgg gcgacgcgat ccagcagctg ttgcgcctcc
     5221 tgacgaccaa acagctctgc ggcatgctgg ctaatgaggt ggttaagatg cgttgctacc
     5281 accgtgctgg cctcaaccac tgtgtacccc tgaatctgcg cctgttcttt tagcgcactt
     5341 tcaatccaga tagcattcag gccaaatgcc ggatcgacgg tcgcctcacc aggtaacgtc
     5401 ccggcagcgg ttccagggtt aatagccagc cagcgccccg gataagcatc accactgcct
     5461 atctccacgc ctttcatcaa aatgcgatag cgggcaggtt gcagatccat attgtcgcga
     5521 atgtgcacca ctggcggcag aaatcccatc tcctgggcaa atttcttgcg gatactgcgt
     5581 atacggccca acaactcacc atcctgctgg aaatcgacca tcgggatcag tcgataaccc
     5641 acttccattc ccagagaatc ttccagttgt acatcgttcc acgtcgcttc gacaacggca
     5701 ttattctctg ccatttttac cggttttggt tcggcaggcg ctttttgttc gcgtccacgg
     5761 atccaccagg ccagccccag caatccggca gtgaacagca aaaataccag gttcggcatt
     5821 ccaggcacca ggccgagtaa accgagcacg gcagcgctta acaacataac gcttgggtta
     5881 ctgaaaagct gattcaccat ctgctcgcca acatcctgat cggtgctgac acgcgtaaca
     5941 atgacccccg cggcggtaga aatcaccaga gccgggattt gtgccaccag accgtcacca
     6001 atggtcaaga gcgtataact ttccgccgcg tgtcccatgc tcatgccatg ttgcagcacg
     6061 ccgaccagca acccgccgac aacgttaatg accatgatga ggatcccggc gatggcatcg
     6121 ccgcgaacaa acttacttgc cccgtccatt gagccgtaaa aatcggcttc ctgagtgact
     6181 tcggagcggc gttttttcgc ctcatcttca ccaatcaatc cggcgttaag gtcggcgtca
     6241 atcgccatct gcttacccgg cataccatcg agaacaaagc gcgcacccac ttctgcgata
     6301 cgcccggcac ctttggtaat gaccataaag ttgatgatca cgagaatgac aaacaccacg
     6361 ataccgatag cgaaattgcc accaacgagg aagtgaccga acgcttcaac caccttccct
     6421 gccgccgctg cgccggtatg cccttccatt aaaatgatac gggttgaagc cacgttaagt
     6481 gccagacgca acagcgtggt aaacaacaaa atggtcggaa acgcagcaaa ctcaagcgtg
     6541 cgctgggtaa acatcgccac cagcaacacc atgatcgaca aggcaatatt gaaggtaaac
     6601 aacaggtcga gtatgaatgc gggcagtggc agcaccatca tcgacaagat caacaggatc
     6661 aaaatcggtc cggcaaggat ctgccattgt gtcgatttca ggtttgtggg caggcgcagc
     6721 atcgcggcca gattactcat gggtcggttt ctcgttaata aaatccaggg cttccggcac
     6781 cggaagatga gtaggttgta cagggcgctg tccaccagcc agacgccagc gtttcagttg
     6841 ccagacccag gccagcactt ccgccaccgc ggcgtacagt tgacccggga tttgttgacc
     6901 aatctccaca tgtcgataca gcgctcgcgc cagcggcggc gcttcaagcg tcgggacgtt
     6961 attttcagcg ccaatttcac gaatgcgcag cgcgaccagc cctgcacctt tagcgaccac
     7021 tttcggtgcg ctcattttgt tttcgtcata ctgcaacgct accgaatagt gggtcgggtt
     7081 attgacaatg acatccgctt tcggcacatc ggccatcatc cgacgccgtg cggcagctcg
     7141 ctgcatctga cggatccgcc ctttaacatg ggggtcgcct tcgctttgtt tgaactcatc
     7201 acgaatatcc tgccgcgaca ttcgcagctt tttcaggtgg ctgaagattt ggaaaaagac
     7261 gtcaaatccc accattggaa tgacaccaag caccaccagc agtgcgcata gccctaccaa
     7321 atccatcgca ttacccatgg cggtaatcgg agactcggcc attaagcgca tcatatgcgg
     7381 ccagtgatgc cagagaaaaa accccgtcac gctgccaacc aggatggttt tcaaaattgc
     7441 tttaagcaac tccgcgccag tctgtgccga gaacatccgt tttatgcccg gtagcgggtt
     7501 gagtttggaa aacttcggct gcaaggattt tccgctaaat accagccccc ccagcataac
     7561 cggagaaatg agcgccacca gcaccacgcc gctaatcagc ggtagcagcg ccagcatggc
     7621 ttctctgatc agcagaataa tttgcccgag gatcagattc gggtctttga taatactgtg
     7681 atcaaaatgc agcccagcgg agagcatgcc cgacaatcga cgggccagcg acacaccgcc
     7741 aaaccagata acactaacgc ccaccagcaa aatcagcagt gaggtcagtt cacgggaacg
     7801 cgggatttgc ccctcttccc gcgctttttc tagtcggtga ggtgtggggg cttctgtttt
     7861 gtcgtcgctc tcgtcagaca cgtcgccaat cctggaaaga gtattaagcg taaatgatgc
     7921 cagagcgcaa agcgttcaat ggtttgagta aggggcaaaa caggcgggat ttagggcttt
     7981 tgctgccaca catcaagcat agtgtgcgtt tgtcagatgc ggcgtcatcg ccttatcaga
     8041 ccgcctgata tgacgtggtc acgccacatc aggcaataca aatcaaaatc caagactatc
     8101 caacaaatcg tccacctgat cctgactggc taccacaccg gctttgctgg tatcgacctg
     8161 aggtccatta agcaaactct ggttttcacg ttttggacgc gactcctgtt ccgggatgtt
     8221 ttccaacagc accatcagca actggcgttc gatctcctga atgacatcca tcatccgctt
     8281 aatgacctgc ccggtgagat cctgaaaatc ctgcgccatc atgatttcca gcagttgcgc
     8341 gttagtaaag ctggtatgcg cgggtacatc tgccagaaat ttccgcgtat ctgttaccag
     8401 ttcacgggcg tcggcaaggt caatcggatc ggcaaaccag tcatcccaac gttgggttaa
     8461 cgcttttgct gatttctcca tttgatcctg atgcggttgt gacgcctcaa cactgttcag
     8521 cgcccgctcc gcagcctggg cggtcatctg cacaacatag tacaaacgat cgcgcgcatc
     8581 ggggatggct tccgccgctt cggcaatggc ctgatccagt cccagttccc gcaaactgtc
     8641 gcgcagcata cgcgtcaggc tgccgatgcg cgcaatgata tcgccagctg aatgctcgtc
     8701 agcaggtttg attgatggtt gcatcatagt cgcatcctca catgcccagt ttctcaaaga
     8761 ttttgttgag tttttcctcc agcgtcgcgg cggtaaatgg cttcaccaca tagccactgg
     8821 cccccgcttg cgccgcagca atgatgttct ctttcttcgc ttctgcagtc accattaaca
     8881 ctggcaatgc cgacatcgcg ccatccgcac gaatcgtttt cagcaattcc aggccatcca
     8941 tgttgggcat gttccagtcg gagataacaa atccataacc gcctgcctgc aacttattga
     9001 gagcgtcgac gccatcttcc gcttcctcaa cattattgaa tcccagctct ttcagcaggt
     9061 tacgcactat gcgtcgcatg gtggaaaagt catccacaac caaaaattta agttctttat
     9121 ccgccatttc acactcctga tttaaatacg tatcgcctgt ccggcactaa tttttgccaa
     9181 catttgctgg cttacctggt taagatcgac cacttcgcag acaccaccca tattgatggc
     9241 ctcgcgcggc atgccgaaca ccacgcaact tgcttcgttt tgcgcaaggg tccatgcccc
     9301 agcctgacgc atcgccaaca ttcccgccgc gccgtcgttg cccataccgg tcaggatcac
     9361 cccaaccgca ttacgccccg cctgtttggc gacagaatgg aacaacacat ctaccgaagg
     9421 ccgatgacgg ttaaccgccg ggccatcgtg aattttgatt tggtaatttg cgccactacg
     9481 cgccagctcc atatgccgat cgcccggcgc aatataggca tgccccggca aaacacgttc
     9541 cccgtcttcg gcttctttaa ccccgatctg gcaaagctta ttaagtctgt cggcaaaaga
     9601 gcgggtgaaa ccgggcggca tatgctgggt aattaacagt gccgggctgg aaagcggcaa
     9661 cggttgcagt acgtgacgaa ttgcctcagt tccacccgtt gaagcaccaa tcgcaatcag
     9721 tttttcagaa ctcaacaacg gccctgcctt cagcgttgtc ggtgccgaca atggcttatg
     9781 tgctgcaagg ctcgcctttg ctgctgtacg caccttttca gcaatcattt cgctgtacgc
     9841 cagcattcct tcgcgaatac ccagttgcgg tttggtgaca aaatctattg cccccagctc
     9901 cagcgcgcgc agcgtgactt ctgatccttt gccggtcagg gaagaaacca tcacaacggg
     9961 cattggacgc aaacgcatta atttttcgag gaaatccagt ccgtccatcc gcggcatttc
    10021 aacatccagc gtcagcacat cgggattgaa tttcttaatc aagtcacgcg cgaccagcgg
    10081 atcaggcgcg gtcgccacca tttccatgtc gctatggctg ttgatgattt ctgtcatgat
    10141 ctggcgcatc agtgccgaat catcgacaga taacaccctg attttgctca tcgttaatcc
    10201 ttacttagcg catacaccgt ttgaccacgc agcgtgaagc ggcgctcgag gtggctaaag
    10261 ttttcagagt gacccgcaaa cagcaatccg tcgggtttaa ggagcggaac aaagcggcgc
    10321 aaaatctcct gctgggtagt ttgatcgaag tagatcatga cgttacgaca gaagatcgca
    10381 tcaaacggcc ccggcacggt gtactgtttc gccagtagat tcagcgaggc aaaatcaaca
    10441 tagttcgcca gctcctgacg cacgcgtacc agcccttcat gcggtcccgt ccctcgcatg
    10501 aaataccgtt gcagttgctg cggcgtcagg tttttcaact cttcatggcg atagataccg
    10561 cttctggctt tttccagcac ttcggtgtcg atatcactgg caaacacttt ccagcgtccg
    10621 ggcgcggtgc ccaatgtgtc agccagcgtc atcgcaatgc tgtacggctc ttcgccggtc
    10681 gaagccgccg cgctccatac gcgatactcg ccagaacggc gacgtgcgtg atccgcgagc
    10741 agagggaaat gatgtgcctc acggaaaaat gccgtcagat tcgtggtcag cgaattgata
    10801 aacgcctgcc actcaccgct gtgctgatta gattccagca agttcagata atgaccgaaa
    10861 tccgtcagtc ccagcgaacg caaacgacga accagtcggt tgtaaaccat gtcgcgttta
    10921 tggtcagcca gaacgatccc ggctcgttga tagatcaatt gacttatccg ccgaaaatgc
    10981 gcgtcggaaa gcgccaggcg ctcggtcatc tgtaacaata aagacgtttg cccacagggc
    11041 agagatgaag tcatagcgcc ttctcaatca cttcaggata ccactggcgc aatttgtaac
    11101 tgcgccgact catgtcgtgc tacttcatgt tcttcaaggg taaataccgc cacacgcgac
    11161 gaaagatggt cggcctgatt cgccagttgt tccgttgcca ccgccgcttc ttctaccagc
    11221 gaggcgttct gctgtgtcac ctgatccatc tggctgacag cctgcgcaac ctgctcaatc
    11281 ccccgccgtt gttcttccga ggccgaagca atttctccca taatgtcgtt cacgcgggtc
    11341 accgaactga caatatcggt catggtcgcg gcggcgttat tcaccagttt cgaaccctgc
    11401 tggacacgat tgactgactc ttcgatcagc cctttgatct cttttgccgc ctgggcgctt
    11461 cggctggcaa gattgcgcac ttctcctgcc actaccgcaa aaccacgccc ctgctctccg
    11521 gcgcgagccg cttctactgc cgcattcagg gccagaatat tggtctggaa agcaattccg
    11581 tcgataacgc tgataatgtc gccaattttt tgcgagctgg tggcgatctc ctgcatggtg
    11641 tgagtcatgg tactgacctg aacaccgccc gcctgcgccg ttgtcgcggc attttttgca
    11701 agttccgacg cctgtcgtgc gttatcggcg ttttgcccta ccgtggcggt taattgctcc
    11761 atactggcgg ccgtttgtgc cagcgatgcc gcctgctgtt cggtacgact tgagagatcg
    11821 ttattgcctg cgacaatctc cgcgatacca atgtgcatct cctggcttcc cttacgcaca
    11881 tcacttaccg tcccacgcaa agcctgctgc atggtcttca ggctggcaaa aatggcggtg
    11941 atctcattac gaccatatac cgcaatcgga cgcgccagat tacccgcagc aatgctgtca
    12001 aaatggctac cgataatggc cagtggttga acaatcatct tgcgcgtcca ccacagcgca
    12061 ctgctgatgt agatcgccgc aacaataatc atgctgataa acaccagcgc cgaaatctga
    12121 tagttacgct ggctttgcgc actggcggct tccagcacat ggatgatctc cagctgccag
    12181 gcctcaaagt taacgtcaaa cgcattctgt gatccctgca ccggcgcagt gaggaaatcc
    12241 gaaagttgat tactttcaag ccaggtcgcc tgatgttcga gatcgttgtg ccagcgggca
    12301 aaacttttct ccgtctcttt ttgcaatgcc ctgacgtgct cgttgcccgc agtcatcgcc
    12361 ataaaacttt taaacagcgt ggtggattgc gtcagactgg cacgcgccgt cgtcatcaac
    12421 gttttaatgt catccgccgg atagctaagc gctgtcagag tgcctgcttt gttcagcgtg
    12481 gtactggcct gtaacattac cgcccgagtt tgcgctaatg ccgcacgttg ctgattgctc
    12541 tgctcgacct gattcagtcg ttgcaaatcg tcgcgaaatg cccaaaaaga catgccgtta
    12601 ctgccaatct gcaagatccc gcagagaatc aaaattaaaa acagcgtggt cgaaattcga
    12661 atacgattaa acatcaacgc tccccatcag gcggcaatga ccgcgttagt aaatactcgt
    12721 caaaatgttt cccagtttgg atcttgttca gcaattcgca gtcgtggctg tgccggtggt
    12781 tgctcactgg caggacggga tggtgtttgc ggtttattgg tgagtgggct ggctgccaga
    12841 cgaaacgcgg aaaccgcttg cgttaaacga ctagcctgtt cttccagcgc agctgcagcg
    12901 gcagctgatt cctgcaccag cgatgcgttc tgttgcgtga cgtgatccat ttccgaaacc
    12961 gccaatgcga cttgatcaat gccacggctc tgttcatccg atgccgatgc aatctcgccc
    13021 ataatgtcag tcacgcgagt gacagcatcg acgatattgt tcattgtttc cccggcgctt
    13081 tcgaccagca ccgaaccggt atcaacgcgc gagacggagt cttcaatgag agctttgatc
    13141 tcttttgccg cctgggcgct acgactggca agattacgca cttcgcccgc caccacggca
    13201 aaaccacggc cctgttcacc cgctcgcgcg gcttcaacag cggcattcag cgcgaggata
    13261 ttagtctgga aggcaatacc gtcgattacg ctgataatgt cggcgatttt cttcgaacta
    13321 tcggcgatct catgcatcgt tttcactacg ccatccacca ctttgccgcc gtgctgggcg
    13381 gtgtcggagg cactttgcgc cagttgcgag gcctggcggg cgttatcggc gttttgcttc
    13441 actgtcgcgg tgagctgctc catgctggcg gcagtttctt ccagcgcgga tgcctgctgt
    13501 tccgtacggg aggaaagatc ggtgttgccc gccgcaattt cacgggtacc ggcatagatg
    13561 gcatctgaac cttcgcggac atgagtgacg gtgtcagtca aagagcgttg catatgtgaa
    13621 acgctctgcg ccaggtcgcc catttcactg cgcccgtcaa tggtcagggt attcgccagg
    13681 ttaccaccgg cgatttcgcg aatgtgagca ataatttttg ccagcggtgt aagcaacata
    13741 cggcgaatgc cataccacgc caccagcaga atcaatacca ccaccagcgc gataaccgcc
    13801 agttgccact gggcaaatcg gtaatcatct gcgttgtcag tgacgatatc gcgatacagt
    13861 ttttcactgc tgagggcgta ctgagcaaac gcttcgccca ttgcattttg cattccctgg
    13921 gttggctgag cgaaataagc tccagtattg ccataatcaa gataatcaat cagttcagtt
    13981 aacgctgtgt aatagttttt atatttttca tcaatattac gactggtagc gaccatttca
    14041 ggtaacggtg ccatgctttt gaatttttta taatgagtcg cggcctgggc caatgttttc
    14101 ctggcgctat cgagcaattc aactttggca ttactttgtt gattggagga atccatcatc
    14161 attcgcaccg ctgaacgact caggttaatg cgcgtttgca gcattaaatc ccaggttgac
    14221 gtcagctcgc cctgctgttc tcgtaattga ttggaaacca caaagctctt ctggctatgg
    14281 tgaagggaag aaaaaaacag actgccggaa ataagctgta acagtgcgaa tacccccagc
    14341 accattacca acagcgtgac tacgcggata cggttaatca taaggcacct tcctgaaaac
    14401 aagttgatct cgttatcggc aaggaagggg gaaactttat tgctgatgcc acccgccgcg
    14461 aaattgaaat aaaacccgat gcgcagatca tcgggttcat ttcaattgag gaaatcagga
    14521 gaattacgcc acttctgacg ccgcgctatc taacagcgcc atctcttcgc tgttcagcag
    14581 tttttcgatg ttcaccagaa tcaacatacg gtcgcccagt gcgcccagtc cagtgagata
    14641 ttctgttgaa agcgtcacgg caaactctgg tgccggacga atttgctccg ccgtcaatga
    14701 aagcacgtct gaaacgccgt caaccacgat gccgaccacc cgctgtccga gattcaggac
    14761 gataactacc gtgttgtcgt tatagtccac atccacctgg ctgaacttaa ttcgtaagtc
    14821 aacaatcggc acaataacgc cgcgcagatt cgtgacgcct ttgataaacg ctggcgtgtt
    14881 cgcaatccgt gttacctgat cgtagccacg gatctcctgc actttcagga tatcaatacc
    14941 gtactcttca tcaccaaggg taaataccag aaattcctgg cctgacggct cgctggccag
    15001 ctttgttaca ttcgtcatac cggtcatatt gttacctttt tactcattca ggcggcggtg
    15061 ttcgccatac gttgttcgcg gtttatcgcc tgcaaggcgg agacatcaac aatcagtgcc
    15121 acgctgccgt cgccaagaat ggtcgcagca gaaatgccgg ggactttgcg atagttactt
    15181 tcaaggtttt taactacaac ctgatgttga ccaattaatt gatccaccag caaggcgtag
    15241 cggcgaccgc cactttgtaa gatcaccaca attccctggg tagcttcggt tttcgcgccc
    15301 gcgacgttga acactttcca cagttcgacg atgggcagat attcaccccg cacttccagc
    15361 acccgctcgc cgccggccag tggatgcaga tcggcttcac ggggttgcag tgattccata
    15421 acagcattca gcggcagaat gaatacttca tccgcaacgc gtacggacat gccgtcgagg
    15481 atggccagcg tcagcggcag taaaatgcgg atcgtagtgc cagtaccctg cttcgactgg
    15541 atttcaacat gaccgcccat ctcctggata ttacgtttaa cgacgtccat gccgacgccg
    15601 cgcccggaga cgtcggtgac ctgctctgcc gtggagaagc ctggtgcaaa tatcagcatc
    15661 gcgacttcgt cgtcgctcat gttttcgctg acagtcaaac cttgcgaggc cgcttttgcc
    15721 agaattcgct cacggtttag ccccgcccca tcgtcggtca cttcaatgca aatgttgccg
    15781 ccctgatgtt cggcagacag aattaaattt ccgacgctgt ttttacctgc ggcgagccgt
    15841 ttttctggca gttcaatacc gtgatcgagg ctattgcgta ccaggtgggt cagcgggtcg
    15901 ataatgcgtt ctatcaggct cttgtcgagc tcggtggaac tgcccaccag cgtcagttct
    15961 acctgcttgc cgagttttcc cgccagatca cgcaccagcc ggggatagcg actaaagaca
    16021 tattccatcg gcatcatgcg aatcgacatc actgattcct gcaaatcacg ggcgttacgt
    16081 tgtaactgcc ccatgctggt aatcaaatca ccatgattaa ccgggtccag ttcgctggaa
    16141 cgctgggcaa gcatggactg ggtgataacc agctcgccga cgaggttaat taattgatca
    16201 accttttcta ccgctacacg gatgctggtg gattcactgc tacgcgtcgt tttttcccgc
    16261 tccacgcgac cggttggcgc ttgttcggct gccagtttaa gcactggtgg ggtggatatt
    16321 tttggcgaga cttctactgt ttcaaaggta atctgatcgg cttcaatcac aaaacagagt
    16381 accgctgtga tgtcatcttc ggcgatatcg cccggtaata ttgccgagag agaatccgcc
    16441 cctttcacca cgtcagttaa cgttgtcaga tgccccagct cttcttccag caggtcgact
    16501 tcgctggcct tcaggcgcga aaggataatt cgtcgcggca actgactgcg actctgctca
    16561 tcttgcggtt cacttttggc aaccacactt aatcgggtca ctgcggatgg cgtttcgcct
    16621 ttcgcttcta atgccagttg acgcaaggcc tggcagatat aatcgaagct ggcggcatcc
    16681 ggctcttgcg actgtttata agcgtcgagc tgttcttgca tgatgtcctt cgtttccaaa
    16741 aacagattga taatgtcggt gttgagttgc atctcacctc gtctggcttc atcgagcagg
    16801 ttttccatca gatgcgtggt ttcctgcaaa acgctgaagc caaaagttcc tgcccctcct
    16861 ttgatcgagt gggcagcccg aaagatggca ttcaattgtt cggcatctgg cgcttccggc
    16921 tgcaaaacca gcagatgctg ctccatatca gccaacagtt cgtccgcttc atcaaaaaat
    16981 gtctgataaa aatcgcttat atccatgctc acgctgtcac ctcggttcgg ctgatggcat
    17041 tgtgggaaca cagacctgtg gtgcaacctc aggtttttcc agggcgctta ctggctcatt
    17101 ctggctttcg gcgttttcat gcaaaatggc ctgttcggct tgtttgttca gtaccagcag
    17161 gctgatgcga cggttgacgg catcatcagg tccgcgatcg cttaagcgca tcgttgccgc
    17221 catgccgacc acacgtaaca ctttgccgct atccaaccct ccgaccatca gttcgcggcg
    17281 ggatgcattg gcccgatcgg cagaaagctc ccagttgctg taacctttct caccactggc
    17341 gtaggggaaa tcatcggtat gacccgaaag gctaatacgg ttgggaatac cattcagaac
    17401 gggcgcgatg gcacgcagaa tgtcgcgcat atagggttcg acgtcggcac tgccagttct
    17461 aaacatcggg cgattctggc tatcgatgat ctgaatacgt agaccttcct ggaccagatc
    17521 gattttgaga tgtggacgta acgcccgcag tttcggatcg gactctatca attggtcgag
    17581 atcgccacgc aatttccgca atcgactttg ctccatgcgt tttttcaact cttcgatgtt
    17641 cggctgctta ttcacttccc cctggctttg ggtgtaatca tcgccaccgc cgggaattgg
    17701 gctttcacta ttagaaatgc gatcgccgcc cgtaaccgca gtcgccagtg gagtccggaa
    17761 gtactccgca atctgaatca actcttttgg gctggagatg gagatcagcc acatcaccag
    17821 aaaaaaggcc atcatcgcgg tcataaagtc ggcataagca atcttccacg atccatgtgc
    17881 tgccccgtgg cttttggctt tgcgtcgttt gacgacaata atcggatgcg cttgattctt
    17941 catgcttcct cggttgtcgt ctgttgttgc ggatttttca ccgcacgcac atgctcttcc
    18001 agttcaataa acgacggacg ttcgctggaa tagagcgttt tgcgaccaaa ctcaacggcg
    18061 ataggcggtg cgtaaccgtt cagattagaa agcagtgtga ctttgacgca ctgcatcatt
    18121 ttgctggttt cggcgctttt ctgacgtaaa acactcgcta atggggaaat aaatccgtaa
    18181 gccaataaaa tgccgaggaa agtccccacc atcgcatgtg cgataagcgc ccccagctcg
    18241 gcggcaggac gatcggctga acctaacgcg tgaacgaccc ccattacagc cgcgacaata
    18301 ccaaacgccg gaagtgagtc cccgaccagc gccagactgt tcgccgggac ttctgcctcg
    18361 ctttcgtgcg tctcaatctc ttcatccatc agggcttcga tttcgaaggt gttcatgtga
    18421 ccgctgataa tcaggcgcag ataatcgacg ataaaatcaa gcatgacgct atccgcgatg
    18481 atgcgtgggt agctggcgaa gatctcgctc tcacggggat tttcaatatc acgttccagc
    18541 gaaaacatcc ccatctgccg cgatttcgcc atcaaccgat aaagcagagc cagcagatcc
    18601 atatacattg ctttggtgta tttggagcga cgaaacagca acggcagcgc tttcagcgtg
    18661 cctttaatcg cttttccatt attgccgaca ataaacgacc caatccctgc accggcaata
    18721 atcaccagtt cagcgggttg atagagtgct ccaaggcttc caccggtcat caaataaccg
    18781 ccgaaaactg taccgagaac aaccaggtaa cctaataaga taagcacgac atcatccttc
    18841 cactgttgac catgacagga tgttcagtcg tcaggcgtta acgcgcgatt ggggcaaaaa
    18901 aaagcagcgg tacgtcgtta ccgctgctgg aatgttgcgc cacaccgtat cagttaaaca
    18961 gcctgtactc tctgttcatc cagcagttgt gggataatat cggcaggatt ctgggaaagt
    19021 ttacgtcttt ttactgcccg ggatggcggt tgacataagc tgcaggcaaa gctgccaaca
    19081 ggctggtgag cgtgggtaat aaaattgccg ccgcagcagt tgcagctgga aagttgcagt
    19141 aatccacttt caacaaaccg caccaatgtc caggcacggg ttaatgccag cagcggtcct
    19201 tcttctgctt gcgggcactg ttcaaggtat aaacggtagg ctttgatcac cgcatcgacg
    19261 ccattacaca aaccggtttt cagtaaaaac tgccatgcat tacagaacat cgaagcatga
    19321 acgttttgtt cccaagtcat aaaccagtcg gttgagaatg gcagcatgcc tttcggcggt
    19381 gggcttccgc gcagttcttt ataaagtttt atcaggcgtc cgcgacttaa ctgtgtttcg
    19441 ctttccagca tctgcaaacg agcgcccagg gtgatcaatt ccattgccag ctgaatatcc
    19501 cgcgcttcct gaacaatgct tttttcactc atgatcaggc ccttttcttg cgcagcgctt
    19561 cttcaggctg attaacatca ttcagcaagc gtgttgagag catgatgccg gtatgaattt
    19621 gctggagatc gtcaacgcgg gaatcttgcg tcaacagagt aatcgtctgg tggctgtcaa
    19681 aacggaagtg acaaaccagc tgattggttt ctgccagctt aaccatttgc ggaagagtca
    19741 gtgccgctaa tgttgtcgcc atttcttcat ttatgccgag acgaaacata gcggacgctt
    19801 tgtcctgaac aatcagacgc tgtgcaagta gtaaatatga caagttgatg tcataaatgt
    19861 gtttcagcaa ctcggaggta tgcattattc ccacccagaa taaccaactt tatttttatg
    19921 cggtttcacc gcaccccgtg atgtcgccgg gaagccccgg taaaaaataa ttagcattag
    19981 aatagttgcg ataagctgca ataagcagag ccaccttttt ggtttaataa gtccttacaa
    20041 atagaagggg gtctttacac ttatctaaga tttttcctaa atcgacgcaa ctgtactcgt
    20101 cactacacgc acatacaacg gaggggggct gcgattttca ataatgtgtg atgcagatca
    20161 cacaaaacac tcaattactt aacataaatg tataagtcat acttttgttt tgggtgtatt
    20221 tcaatctgtt aaaaagtttt tcgctacgct agcaagcaaa aatgaaacag gaataatcga
    20281 aatgggatgt tgcgcacagt taaaataact caccgtaaat aatcatctgc tataaataat
    20341 cactttcatg caataccaga taagctattt ttaaacagac acttaccgca caacaaacta
    20401 attaaataca aagaaaggca acaatcaact taatgttaat aaaat
//
LOCUS       DAZKIP010000075        20183 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZKIP010000075 DAZKIP010000000
VERSION     DAZKIP010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20183
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.075"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            236..736
                     /gene="tssB"
                     /locus_tag="ACHOUK_003041"
     CDS             236..736
                     /gene="tssB"
                     /locus_tag="ACHOUK_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFP9898438.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSSLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDEMNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GQVSNK"
     gene            771..995
                     /locus_tag="ACHOUK_003042"
     CDS             771..995
                     /locus_tag="ACHOUK_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9898439.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1046..2437
                     /gene="tssC"
                     /locus_tag="ACHOUK_003043"
     CDS             1046..2437
                     /gene="tssC"
                     /locus_tag="ACHOUK_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFP9898440.1"
                     /translation="MSLQEEELVSSHAGQPEQESSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYYEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAK
                     VEVMDVEGEPGWY"
     gene            2528..2941
                     /gene="tssE"
                     /locus_tag="ACHOUK_003044"
     CDS             2528..2941
                     /gene="tssE"
                     /locus_tag="ACHOUK_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFP9898441.1"
                     /translation="MILDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2945..4795
                     /gene="tssF"
                     /locus_tag="ACHOUK_003045"
     CDS             2945..4795
                     /gene="tssF"
                     /locus_tag="ACHOUK_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFP9898442.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4759..5841
                     /gene="tssG"
                     /locus_tag="ACHOUK_003046"
     CDS             4759..5841
                     /gene="tssG"
                     /locus_tag="ACHOUK_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFP9898443.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTTFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5866..7146
                     /gene="tagH"
                     /locus_tag="ACHOUK_003047"
     CDS             5866..7146
                     /gene="tagH"
                     /locus_tag="ACHOUK_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFP9898444.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHAPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDHNDDISAAEMAQRHLAVTPLLRGLGGSLTVSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7143..7667
                     /gene="tssJ"
                     /locus_tag="ACHOUK_003048"
     CDS             7143..7667
                     /gene="tssJ"
                     /locus_tag="ACHOUK_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFP9898445.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLQAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPIKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7670..9001
                     /gene="tssK"
                     /locus_tag="ACHOUK_003049"
     CDS             7670..9001
                     /gene="tssK"
                     /locus_tag="ACHOUK_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFP9898446.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHRARRSQLHPEDFYLDLAELLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADVH
                     DSELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            9006..9767
                     /gene="icmH"
                     /locus_tag="ACHOUK_003050"
     CDS             9006..9767
                     /gene="icmH"
                     /locus_tag="ACHOUK_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFP9898447.1"
                     /translation="MDEGSLSLPPFTGYDEKSLRDYHLALHGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWLCFSLGFR
                     GRYKVAVQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9776..12553
                     /gene="tssH"
                     /locus_tag="ACHOUK_003051"
                     /gene_synonym="clpV"
     CDS             9776..12553
                     /gene="tssH"
                     /locus_tag="ACHOUK_003051"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFP9898448.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTLTDASDTLLARYAKNMTEDARNGRLAPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYERSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESESESESEQDNTGAVPADETDREQPEETAETVSPVQRLAQLT
                     AELDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLPKWLGDTIKG
                     QDLAIASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQYLT
                     TINMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLN
                     LFYQAFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEALYPVLADF
                     FKPALLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVVIEPEVTDEIMSR
                     VTRAENGARMLESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAVDGAFTADVEDAQN
                     DESVTKDETVL"
     gene            12550..13293
                     /gene="vasI"
                     /locus_tag="ACHOUK_003052"
     CDS             12550..13293
                     /gene="vasI"
                     /locus_tag="ACHOUK_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFP9898449.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDHLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADSRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13298..14710
                     /gene="tssA"
                     /locus_tag="ACHOUK_003053"
     CDS             13298..14710
                     /gene="tssA"
                     /locus_tag="ACHOUK_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFP9898450.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPDWEYIDGEIVK
                     LGSLAHAQLDIPELQRRGLQLLASESKDFRLLAHLLRTLQHAGDPLLALHLLTLYVEH
                     YWTVAAPQNMAHKKRFASQIIKRFETGIEGFSQNAATTQRDTLLGELAKLAQCWQSHN
                     VPELAQATDDLFALYQRTFNRAAPAPVPTPAASGSSPQTTVTSESGVTQPSAPAPQIA
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQSITSTPQAESDGRTPLAA
                     VSADMVADYQSRLASADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADTIR
                     DEVIRFLERLPQLTGLLFNDRTPFLSEQTKQWLAASPDGKVAPVAQIGEESQAARACF
                     AGQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLIQLAQQQYRMLLMIGSQMM
                     VSDWEPSLLTQLEQKFTAEQ"
     gene            14847..18281
                     /gene="tssM"
                     /locus_tag="ACHOUK_003054"
     CDS             14847..18281
                     /gene="tssM"
                     /locus_tag="ACHOUK_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFP9898451.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALVWLTVRVMKRLQLLEKQQKQQREEAIDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGTEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRHREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVTQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLMRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDYGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFLTRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQRQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KERDEALAEPDYQLLTRLGHEFAPENITLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLTDQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            18292..19701
                     /locus_tag="ACHOUK_003055"
     CDS             18292..19701
                     /locus_tag="ACHOUK_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFP9898452.1"
                     /translation="MNSNVLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAESDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQNTRKRLEPQLKSNTENAAQTTVRTLIYAPETQASSTPEAVVPPLPGLPEMK
                     VEVRSLTENPPQASVIKQGSTVRGFIAGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLDTSPVQPLETGMQMMRVADSRWPESLQQQQASTQ
                     WNEALKTRAQSSPQLRGWLQTRQDLHAFADLVMQREKEGLTLSYIKNVIWQAERGLGQ
                     ETPVESLLTQYQDARAQKQNTDALEKQINERLEGVLSRWLLLKNNTIPTIKKALNFNN
                     IHEYKGVLNGEFNLFNTKW"
     gene            19667..20146
                     /locus_tag="ACHOUK_003056"
     CDS             19667..20146
                     /locus_tag="ACHOUK_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFP9898453.1"
                     /translation="MANLIYLTLNGDNQGLISSGCSSQPSIGNKAQTAHIDQIMIYEF
                     MHGLNRDQNVNHHHLTIKKPIDKASPLLGKAICDNELLTCDFSFYRTNKFGINELFYK
                     IKLTGAKISDIHVSISHIVVDNSVQPEESVSFSYESIIWEHCSAGTSAYSLWEDRLF"
BASE COUNT         4767 a         5524 c         5554 g         4338 t
ORIGIN      
        1 aatacacctg acgcgaattg cacaggttca taaaatctat ttaacgcttg cagaataccc
       61 ctctttacga tcgataaaaa gtccttttct tatttatttt gaagcgtaaa atgcggggtg
      121 atgaaggagt ggtgatttta ttttcaccgc catcaagacg gcatttccga tattttttgc
      181 ggtacgaata tgccgtgttc atggcctgta cgagataaca tgacggatca cttttatgag
      241 caaaaaattt gaaggcagcg tcgcgcctcg cgaacgtatt aatatatcgt atgtcccaaa
      301 aacagatggc cagacggctg aagtggaact tccgcttaac atgctcgttg tcggcgatac
      361 cggaaatacg caggaaacat cctcactgga tgaacgtcag gcagtttccg ttaacaagca
      421 caatttcggt gcagtgatgg ctgaagcagc cattggcctc aattttaccg tccccgccac
      481 gctgaaggga agcacaacgg acgatgaaat gaatgtggcg ctgaatatca agtcactgga
      541 tgatttttca ccagatagcg tggcccggca ggttccggaa gtgaataaat tgctggaact
      601 gcgtgaagca ctgactgcac tgaaggggcc gatgggtaac cttccggcat tccgtaccca
      661 gctccaggca ttgctggaaa atgaagagtc ccgcgagcag ttgctcaaag aaatcggcca
      721 ggtgagcaat aaataaccac cactatttca ttaaacaggg ttatccaggc atgacggcac
      781 acaaaaatat ctccgggaca taccacctct ctgttgcaga cattctgcag gtggtttacc
      841 aggtatgttt cagcccgtcc gttgaaatca accaggatgg tgtggctgcg ctgattacaa
      901 cactggatcg ccgtatcagt gatctattgg atgagattat ccatttttgt gagtttcagc
      961 agagcgcgtc gcactggcag cgcgtacttc actgacggcg aaaagccggt ttcagaaaca
     1021 cagctaaaac agaaggattt tcagtatgtc tttacaggaa gaggaactcg tttccagtca
     1081 tgccgggcag cctgagcagg aatcgtcgct gcttgatcaa atcatggcac agacccgtat
     1141 tcagcccggt tcggaaggtt atgacgttgc ccgccagggt gtgacagcct ttattgccag
     1201 catcctgcaa tccactgcat ctgccgaacc ggtcaataaa ctggccgtgg acagcatgat
     1261 tgcagatatt gatgaacgca tcagccgcca gatggatgtc atcatccatg cgcctgcctt
     1321 tcagcaggtc gagtcgttct ggcgctcact gaaaaccatg gtggatcgcg ttgatttccg
     1381 tgaaaacatc aaggtcaacg tgcttcatgt caccaagcag gaacttctgg aagactttga
     1441 gtttgcgcca gaaattattc agtccggatt ttataagcac gtttattcgt ccggtttcgg
     1501 acagtttggc ggtgaaccca tcgctgccgt gctgggcgct tatgagttca aaaataccgc
     1561 gcctgacatg aaactgctgc aatacgtcag tgccgtgggc gcaatggcgc acgcaccgtt
     1621 cctgtcctcc gtttccccgg agtttatggg gctgaactcg tggaccgaac tgcctaatat
     1681 caaagatctt tatgccatct ttgaaggtcc ggcctacacc aaatggcgcg ccctgcgtga
     1741 ctcggaagat tcgcgctatt tagggcttac ggcaccccgc ttcctgcttc gccagcccta
     1801 ttcaccaaca gataaccctg ttaagaactt taactattac gaagatgtca gccagaacca
     1861 cgaagattat ctgtggggta atacggcctg gatgctggca tgcaatatcg cagacagttt
     1921 tgccaaatac cgctggtgtc caaacattat cggtccgcaa agcggcggcg cagtgaaaga
     1981 tctgccggtg catctgttcg aaacgatggg gcagattcag gccaaaatcc caaccgaggt
     2041 gctggtcacc gatcgccgcg aatttgaact ggctgaagag ggttttatca cccttaccat
     2101 gcgtaaagac tctgataatg cagccttttt ctctgcaaac tcggtacaaa aaccgaagca
     2161 ctttccggga aaagatgcag aaaccaacta taagctgggt acgcagcttc cttatctctt
     2221 catcatcaat cggttagcgc actacatcaa ggtgttgcag cgtgaacaac tggggtcatg
     2281 gaaagaaagg agcgacttag aaagagagct taatacctgg atccgtcagt atgttgccga
     2341 ccaggaaaac ccgcctgcag acgtgcgcag ccgcaaacct ctgcgcgctg ccaaagttga
     2401 ggttatggat gtagaaggcg aacccggctg gtactaggtt gcgctaagcg tgagacctca
     2461 ttttaaattt atgggggcaa attttgagct ttccctggtt ggccggttag acagggagta
     2521 acacaccatg atactggatc gggaaagaac cacaggttct ctttttgagc gtatggaagc
     2581 ctcttcggcc agaaaccggc agggaggcag tattcattcg ctcaggcaat ccattcgcca
     2641 gaacctaaga aacattctta atacccggtc cggtagttgc cggggtgcgc ctgagctggg
     2701 cattgatgaa ccggaagggg ctgaaaactt ccgcgaatcc atgagtcggg ccatcgagca
     2761 atgtatagaa cgctatgaac cccggatctc acacgctgaa gtgcaggcag tggtttcatc
     2821 cgcctccagc ccgctggata tgacgtttca tattacagcc tgggtgacat ttaacgaaac
     2881 gcatgaggtg cttgaatttg atatggcacc gaatggcagc cagcattacc gggtggactg
     2941 acaaatggaa tttgaagaac gctattttcg ggaagaactc gattacctgc gccagcttag
     3001 caagctgctg gcaacggaaa aaccccatct ggcccgcttc ctggccgaaa aagatgcgga
     3061 tccggatatt gaacgcctgc tggaaggggt ggcttttctt accggcaatc tccgccagaa
     3121 aattgaggat gaattcccag aactgacgca cgggcttatt aagatgctat ggcctaatta
     3181 cctgcgtccg gttccggcaa tgacccttat tgaatatacg ccggatatgg ataagtcttc
     3241 tgtaccggtg ttaatccccc gtaatgagca gtttacaacc aacgccgggg aaatcagagt
     3301 tgatgaagtg ctgccctctg atgctaaaaa ggaggagccg cctccctgta ccttcaccct
     3361 ctgccgggat atctggctgc tgcccgttcg cctggagcag attgaaaacc gcagtacgac
     3421 ccgtaatggt gttatcaaca tcaccttttc ggtcgcaccg ggaacggact tccgcacgct
     3481 ggatctgaac aaacttcgct tctggctcgg caatgacgac aactataccc gtgaccagct
     3541 ttatttatgg ttctgcgaat acttgcaggg tgccgacctg actgtgggtg aacagcatat
     3601 tcgcctgcct gagtttatgc taaaagctgt cggttttgag ccgcaggatg ccatgctgcc
     3661 ctggccgaaa aacgtccaca gcggctaccg gatccttcag gagtatttct gttaccccga
     3721 tgcgtttctc ttttttgatc tttgtggttg tccggctttg cctgacggat tgcaggcgga
     3781 attctttacc ctgcaactgc gtttttcgcg ccctttgccc gtggacatcc ggctgcgccg
     3841 cgattccctg cgcctgtatt gcgcacctgc cattaattta tttatccacc atgcagaagc
     3901 catcacgctg gacaaccggc gggcagacta tccgctggtt cccagccgcc attacccaca
     3961 acattacgat gtattttccg ttaacagtgt ggtgagccag gtccaggata tgttcaggaa
     4021 aaaagatctg gggcgtcctg tttcgacgca ggccgcgcgc cagtggccag cctttgaaag
     4081 tttcagccat cagatggaat acagccggaa gcgggaggtg gtgtactggc atcaccggac
     4141 caaaacatcc ctgttccatc gcggctttga tcataccctt gcctttatac atgctgatgg
     4201 cagttatccg tcagacgaat ctctgctcag taatgaagtg gtttcggtat cgctgacctg
     4261 taccaaccgt gagcttccgt cacaaattcg ttccggcgat atcaccggca caaccggtaa
     4321 aaatgcggct gttgcttcct ttcgcaacat tacccgcccg acgcaaccac tctggccggt
     4381 cattgatggc agcctgcact ggtccctgct ctccgccatg aacctgaatt atctgtcatt
     4441 actggatacg gacgcgctga agcaggtcat cgccaacttt gatcgccacg caatccacca
     4501 tccgcagacg gcgcggctgt cacaacaaaa gctggatgcc attgagcgtc tggagacccg
     4561 ccccgttgat cgcctgttta cgggtattcc cgtccgggga ctggcctcca cgctgtatct
     4621 gcacccggag ccgtttgtct gtgaagggga aatgtatctg ctcggtacgg tgctttcgca
     4681 ttttctgtcg ctgtacgcca gcgttaactc attccacatg ctgaccgttg tgaacacaga
     4741 aagccaggag acatggaaat ggacggaaag aatcgggcag catcctctta tctgagtccg
     4801 ggaaatcccc cggcagataa agagcagaat gatccgctgg cacaggtttt ccataatgcc
     4861 tgctcttaca atttttttgc gatggcggag ctgttgcacc gcctggcaaa gggtgaaaag
     4921 gggacgccag aattatccct gcgcgacgat ccggcacagg aaaccctgcg tttcagcgcc
     4981 gatgccagcc ttgccttccc gtgcagtgat atcagtgcgc tgaaacggga tacgtcaggg
     5041 gcattcagga tgaccactac ctttatgggg ttacagggga gccagtcgcc tctccccggt
     5101 tattacctcg atcacctggc ctggaaagcg gtacatgaac aaagtccggt aggtgatttt
     5161 ctggatatgt tcagccaccg cctgacccag tttgtctggc atatctggcg taagtaccgc
     5221 tatcacatca gttttcgtaa cggcggtgtg gatgcgttct cgcagcgcat gtactccctt
     5281 gtcgggctgg gccaccgcca gcttcgcgat aagctggcga ttaatcacag caaaatgctg
     5341 gcgtattccg ggatcctggc aaatccggga cgatccccgg aaattatctg tggcctggtg
     5401 tcccactgct ttgatttgtc agaagtgacg ctgcaaaact ggcaacgtcg caaggtggat
     5461 attgaaccgg atcagcaaaa cagcctgggg agctattctc ttaaaaatgg cgaaaaactg
     5521 gcgggaagat cggtactggg caactttgtt ctgggtacac gagtacccga tctcagcgga
     5581 aaattccagc tcagtattac cagcctgacc cgaaaacagt tcctctcttt tctgccgtcc
     5641 ggcgaaaact tcctgccact gacgacgttc gtgtccttca tcctgcgcga tcagcttgcc
     5701 tgggacttac atctggggct ggcgccggag caggtgggcg caatgcgcct gggcgataac
     5761 aaaagtgcgc tgctgggctg gaccagcttc ctcggcacac cggaagaacg accctcagtc
     5821 accatcaggg tacggtcgta aattatgata atcacggagc agaatatgcc ggaagagaaa
     5881 ctacagacgc tttcattgca ggtcattaac ggcagtgagc tggaaagcgg gcgggcggcg
     5941 cgctgtctgt tcacacagca gggaaatgtc ggccatgccc ccgaatgcca ctggtcggta
     6001 caggatcgtc agcagagcat tccggcccag gcttttaccg ttatcctgca cgatggcaca
     6061 ttttgtctac gcccgcagac ggcacaactg tggctgaatc aggcaaaagt cacagcaaca
     6121 tcagacctga tacagttgcg ccagggcgat gagatccaga tcggacggct gatggtgagg
     6181 gttcatctga accggggaga tattccccat tacgatgagg aaatggccac tcccgaaacc
     6241 atcgttacca atcgcgatat gctcacggat accctgctat caacggaggg tgcgccacac
     6301 tatccgggaa tgactcaccg gcaccagctt gcagacaccg tggtaaatgg tttttctgcc
     6361 gatccactcc aggcacttca gtccgaaagc ctgattacca cgggcgatcc gctttcaggc
     6421 attgcggctg tccggccatc ggcaccgctg tccgatccgg caagtaatgg ggggatcaat
     6481 actccgttta tggatctgcc gcccatttat gccagccctg gcgatcataa tgatgacatc
     6541 tctgcggcag aaatggcgca acgccacctt gcggtcaccc ccttactgcg cggtcttggc
     6601 ggctcgctta ccgtgagcaa ttccgacgat gcggatgatt ttctggagga ggccggacga
     6661 acgttacagg ccgcaataaa aggtctgctc gatttgcagc agcagcgtaa cagcctctca
     6721 gacaaacatt tgcgcccgct ggaagataac ccgctgcgcc tgaacatgga ttacgccacc
     6781 gcgctcgacg tgatgtttgc cgaaggtaaa agcccggtac atctggcggc tcccgccgcc
     6841 gtcagtgaaa gcctgcgcaa tgtccgccac cacgaagaag ctaaccgggc ggcgattgtg
     6901 gagtcgcttc gtgtcctgct ggatgctttc tcaccacaaa atctgctgcg ccgctttgtg
     6961 cagtaccgcc gcagccatga actgcgccag ccgctggatg atgccggagc atggcaaatg
     7021 tacagccatt attacgaaga actggcctcc gatcgccagc aggggtttga gatgctgttt
     7081 aacgaggtct acgcccaggt ctatgaccgg gtgcttcgtg aaaaacagcg ggagccggaa
     7141 gcatgaacag gaaggcgttt ctggcctgcg tgctgatgtg cgtcatatta accggctgcg
     7201 aaacggcgaa aaaaatcagc gaggtgatca aaaacccgga tatccaggtc gggagcctga
     7261 aggagcaacc ttcggaaata accgtcaccc tgctgactga accggacacc aataccaatg
     7321 cggagggtga atcagccgcc gtggatgtgc aactggtgta cctgactgac gactcaaaat
     7381 tgcaggcagc cgactatgac cagatagcca gtacgccgct accggatgtg ctgggtaaaa
     7441 actatatcga ccaccaggat ttcaacctgt tgccggacac catcaaaacc ctgccgccga
     7501 taaagctgga tgagaaaacg cagtttattg gtgtcgtggc ctatttctcc gatgaccagg
     7561 ccactgaatg gaaacaaatc gagacggtgg aaggaaccgg ccaccattac cgcctgctgg
     7621 tgcatgtacg tcaaagcagc atcgaaatga aaaaagagga tgaataatta tggcaaccac
     7681 ccgcaacaag gtgatgtggc aggaagggat gctgatgcgc ccacaccatt tccagcaaca
     7741 gcagcgttac aacgactacc tggataacca gcgtttccgg gccatgaatg atttatcctg
     7801 gggatttacc gaactcaccc tcaacaatga actgctggcg cagggtaaga tcatgattga
     7861 cagcgcgtca ggcacactgc ctgacggcac cgtcttttct atccccgacc aggacgcact
     7921 gcccgatccg ctgcacccgc aaaattttcc ggacgaaaga agccgcaata tttacctcgc
     7981 tctgccggtc gccagtgatg tgagaaatga aatcagcgac gggcggcgaa tcgggcgtta
     8041 ccggctgaat tatgccgatg tccgggatct gcattcagaa gaaggcgacg cgcgaacact
     8101 gacgctggga caactgacgc cgcgcattat gagcggtgca gaagatatga gtgcctatat
     8161 tacgctcccg ctttgccgta tcagcgatcg ccatgccgac ggctccctga cgctggatga
     8221 cgattttatc ccctcctgcc agaatattca ggtcagtaag aaactgcgtg tttatctcaa
     8281 agaggtacag ggggccattg gcggacgggc aagcgatctg gcaaaccgta ttggctctcc
     8341 ggcgcagagc ggcatcgcgg atgtggcgga atttatgatg ttgcagttgc ttaaccgtaa
     8401 ccagacccgg tttacccatc gcgcccgccg atcccagctc cacccggaag atttctacct
     8461 tgatcttgcc gagttgctgg gtgaactgat gacctttaca gagccgtcac gcctgccctg
     8521 cccgcttgat gtgtatgatc atcatgacct gaccaaaaca tttaaaacac tgttaccgga
     8581 agtcaaacgg gcgctgcata ccgtactgtc gccaagagcg gtcaatctgc cgctgcatct
     8641 gcgtgacggc atctggcagg ccgatgttca tgactcggaa ctgctacagt ctgccacctt
     8701 tgtactggcc gtggcagcaa atatgcctgt cgatcagatc cagcgtcagt ttatccagca
     8761 gtcgaaaatt tcctcgccgg aaaaaatccg caatatggtc agtgtgcaga tcccgggtat
     8821 tcctctgcgc gcactgatgg tggccccccg ccagcttccc taccattccg ggttcagcta
     8881 tttcgaactc gacaagagcg gccaggcctg gacagaaatg gctgccgccg gggccgttgc
     8941 actgcatgta tccggcagtt tcccggatct gaacatgcaa ctgtgggcga taagagggta
     9001 agacgatgga tgaggggtca ctgtctctgc caccatttac cggatacgac gaaaaaagcc
     9061 tgcgcgacta ccatctcgcg cttcatggca acagcctgaa tccgatgatc gacgcggcga
     9121 cgccgctact cggtatggta atgcgccttt ccaccatgaa cagccagacg atgccggagc
     9181 atctttttgc tcaggtggtg acagatgttc aggccgtgga gcaactgctt caggaacagg
     9241 gctacgaacc gggcgtgatt atctcgtttc gctacatcct ctgcacgttt attgatgaag
     9301 cggcgctcgg taacggctgg tcgaacaaga acgagtggat caagcagtca ttgctggtcc
     9361 atttccacaa cgaagcctgg ggtggcgaga aagttttcat cctgctggag cgcctgatcc
     9421 gtgaacccaa acgttaccag gatctgctgg aatttctctg gctctgtttt tctctgggat
     9481 ttcgcgggcg ctacaaagtg gcagtacagg atcaggggga atttgagcag atctaccgcc
     9541 gtctgtacca cgttctgcat aaactgcgcg gcgatgcccc tttcccgctg ttacaccagg
     9601 acaaaaaaac ccaaggcggg cgttatcagc tcatcagtcg cctgactgtt aaacatattt
     9661 tttgtggtgg cgttgtcgtg ctggcgttgt tttacctgtt ctatctgttg cggctggaca
     9721 gccagacgca ggacatcctg caccagctaa acaaattact tcgataagga cgtttatgat
     9781 ccagattgat cttcccacgc tggtaaaacg gctgaacctg ttctcccgcc aggcgctgga
     9841 gatggccgcc tcagaatgta tgagccagca ggcagcggaa attaccgtca gccatgtgct
     9901 tattcagatg ctcgccatgc cacgcagtga cctgcgggtt attacccgcc agggcgatat
     9961 tggcatggaa gagttgcgcc aggcgttgac ggtagagaac tacacaaccg cccgttctgc
    10021 ggacagctac ccggcgtttt ccccgatgct ggttgagtgg cttaaagagg gctggctgct
    10081 ggcgtcggct gagatgcagc acagcgaact gcgcggcggc gtgttgctgc tggccttgct
    10141 gcattcgccg ctgcgttata taccgcctgc tgccgcccgg ctgttgaccg gcattaaccg
    10201 tgaccgtctg caacaggact ttgtgcagtg gacacaggag tcggcggaat cagtcgtgcc
    10261 ggatgcagac ggtaaaggcg caggcacact gacggacgcc tctgacaccc tgcttgcccg
    10321 ctacgccaaa aacatgacag aagacgcccg taacggcagg ctagccccag tactgtgccg
    10381 cgaccacgaa atcgacctga tgatcgacat tctctgccgc cgccgtaaaa acaacccggt
    10441 ggtggtgggc gaagcaggcg tgggtaaaag cgcgctgatt gaagggctgg cgctgcgcat
    10501 cgtggcaggc caggtgccgg acaaactgaa aaacaccgat atcatgaccc ttgacctggg
    10561 cgcattgcag gccggggcat cggtgaaggg tgagtttgaa aaacgcttca aggggctgat
    10621 ggcggaggtc atttcctctc cggtgccggt cattctgttt atcgacgaag cgcataccct
    10681 gattggcgcg ggcaaccagc agggcgggct ggatatctcc aacctgctga aaccggcgct
    10741 ggcgcgtggc gaactgaaaa ccatcgccgc caccacctgg agcgagtaca aaaaatactt
    10801 cgagaaagac gccgccctgt cgcgccgctt ccagttagtg aaggtcagcg aacccaacgc
    10861 tgccgaagcc accattattc tgcgtggtct gtcggcggtc tatgaacggt ctcacggcgt
    10921 gctgattgac gatgatgcct tgcaggccgc agcaacgctg agcgaacgtt atctctccgg
    10981 gcgtcagtta ccggacaaag cgattgatgt gctggatacc gcctgcgccc gtgtggccat
    11041 caacctgtcg tcgccgccga agcaaatctc ggcgctgacc actctgagcc accagcagga
    11101 ggcggaaatt cgccagcttg agcgcgagct tcgcatcgga ctgcgtaccg acacatcacg
    11161 gatgaccgag gtgctggtgc agtatgatga aacgctgacg gcgctggatg aactggaagc
    11221 ggcttggcac cagcagcaga cgctggtccg ggagattatc gcgctgcgcc agcagttact
    11281 gggcgtggca gaggacgatg cggcgccgtt gccggacgca gatactgtgg aggatacgca
    11341 gccagagtca gagtcagagt cagagtcaga acaggataat accggtgccg taccggctga
    11401 tgagaccgac agagaacagc cggaagagac cgctgaaaca gtttccccgg tacagcggct
    11461 ggcccagctc actgccgaac tggacgccct gcataacgac cggttgctgg tctccccgca
    11521 cgtcgataaa aaacagattg cggcggtgat tgccgaatgg accggcgtgc cgctcaaccg
    11581 cctgtcgcag aatgaaatgt cggtcatcac cgacctgccg aaatggctgg gcgacaccat
    11641 caaaggccag gacctggcga ttgccagcct gcataagcat ctactgaccg cacgcgccga
    11701 cctgcgtcgt ccgggacgcc cgctcggtgc gttcctgctc gctggcccca gcggcgtggg
    11761 taaaaccgaa accgtcctgc aactggcaga gctgctctac ggcggtcgcc agtacctgac
    11821 caccatcaat atgtccgagt tccaggagaa acacaccgtt tcgcggctga ttggctcccc
    11881 tccgggctat gtcggctatg gcgaaggcgg cgtgctgact gaagccatcc gccagaaacc
    11941 ctactcggtg gtgctgctcg atgaagtgga aaaagcgcac ccggatgtcc tcaacctgtt
    12001 ctaccaggcg tttgacaagg gcgagatggc cgacggcgaa ggccgcctga ttgactgtaa
    12061 aaacatcgtc ttcttcctca cctccaacct cggttaccag gtgattgtcg aacacgcgga
    12121 tgacccggaa accatgcagg aagccctcta tccggtgctg gcggacttct ttaaacctgc
    12181 cctgctggcg cgaatggagg tggtgccgta cctgccgctg tcgaaagaga cgctcgccac
    12241 cattatcgcc gggaaactgg cccgtctgga taacgtgctg cgcagtcgct ttggtgcaga
    12301 agtggtcatt gaaccggaag tgacggacga aatcatgagc cgcgtcaccc gcgcggaaaa
    12361 cggcgcgagg atgctggaat ctgtcatcga tggcgacatg ctaccgccgc tctcgctgct
    12421 gctgttgcag aaaatggcgg ctaacacggc gattgcccgg attcggttgt cggcagtgga
    12481 cggcgcattt acggcagacg tggaagatgc tcagaacgac gagtccgtca caaaggatga
    12541 aacggtttta tgagcaggtt gcgcgttttc cggcagacag gatggtttat cgcgggcctg
    12601 atgacgggct tacccgccac tgccgcgccc gcagaagcat cgtcaatggc cggtgtggcg
    12661 gtcgccgtcg ccaccacgac cccgccggat gcgacagcca cgttacaggc catgcagtcc
    12721 tgtcggcggg agtccgcggc gctggagcgg cttgactgct atgaccacct gctggcaccg
    12781 ctgtccccgt cagggtttga cggggcgctg gtcaaagccg gttttgtggg cgaggcctgg
    12841 acccgcgcca cagagcagga aaaacgccgc gagggcaaca ccacagaact gctggtgacg
    12901 caggtgccgg gggagcgccc gacggtggtg ataaccaccc cggctatcgg ccatgttccg
    12961 ccgcgcccgg tgctgatgtt cagttgcgtg gacaatatca cccggatgca ggtggcgctg
    13021 atgcaccctc tggatgtcca tgatatcgcc gtcactctga atgccgacag ccgggcattg
    13081 cgcagccact ggtttgtgcg cgagaacggc accctgctgg agtccagccg ggggctgtcc
    13141 gggattgatg aaattaagca actgttcggc gcaaaaacgc tgacagtgga taccggcgca
    13201 gataacgccg caggaaagct gacctttaac attgacgggc tggcaagggc gattgccccc
    13261 ctgcgcgatg cctgccactg ggcgggagaa taaaaatatg gcaatggact tacgcgatcc
    13321 gaatgtctgg atatcgcacc tgctggaaaa cctgccggaa gaaaaactgg catcggcgct
    13381 gaaagatgac aacccggact gggagtatat cgacggcgaa atcgtcaagc tggggtctct
    13441 ggcccacgct cagcttgata ttcccgaact acaacgcagg gggctacagc ttctggcttc
    13501 tgaaagcaaa gacttcaggc tactggcaca cctgctcaga accctgcaac atgccggtga
    13561 tccactgctg gcactgcacc tgctaacgct atacgtggaa cattactgga ctgtggccgc
    13621 gccgcagaat atggcgcata aaaaacgctt tgccagccag atcattaaac gttttgaaac
    13681 gggtattgaa ggcttttcac aaaacgctgc cacaacgcag cgcgatactc tgctgggtga
    13741 gctggcgaaa ctggcgcagt gctggcagtc acataacgtc ccggaactgg cacaggctac
    13801 cgatgatctt tttgccctgt accagcgtac gtttaatcgt gcggctcctg ctccggtccc
    13861 cactccggcg gcctccggta gttcaccaca aaccaccgtc acgtctgaaa gcggcgtgac
    13921 gcaacccagt gctccggctc cccaaatcgc catcgacagt cacgacgaca aagcctggcg
    13981 cgacacgctg ttaaaagtgg cggctatttt atgtgaacgc cagccggact cgccgcaggg
    14041 ctatcgcctg cgccgccatg ccctgtggca atccatcacc agtacacccc aggcggaaag
    14101 cgatggacgt accccactgg ctgcggtctc tgccgatatg gtggcggatt accagtcccg
    14161 gcttgccagc gcggatatgg cgctgtggca acaggttgag aaaagcgtat tgctggctcc
    14221 ttactggctg gacggtcact gtctttctgc acagacggca ctgcgtctgg gttacaaaca
    14281 ggtggcagac accatccgcg atgaggtcat ccgcttcctt gagcgtctgc cccagcttac
    14341 cgggctgctg tttaatgacc gcacaccgtt tctcagtgag cagacgaaac aatggctggc
    14401 tgcttcgccc gacggcaaag ttgcaccggt tgcgcaaatc ggtgaggaat cgcaggcagc
    14461 cagagcctgt tttgctgggc agggtctgga ggcggcgctg cgatatctgg acatgctacc
    14521 cgaaggcgat ccccgcgatc agtttcaccg ccagtacctt gccgcacagt tgacggagga
    14581 ggcggggctg atacagcttg cgcagcaaca gtaccggatg ttgttgatga tagggagtca
    14641 gatgatggtg tctgactggg agccatcatt acttacgcag cttgaacaaa aattcacggc
    14701 agaacaataa aagagaacag gagtttttgt gttcagatta ccaacacccc gactactcag
    14761 cggactcaaa tcagccctgc gaccggcgat gccccggttt aaagtctccg ctttctggct
    14821 gctgatactg gcgtggattt ttctgcttgc tgatactggc gtggattttt ctgctggtat
    14881 ggatctggtg gaaaggcccg acgtggacgc tgtatgaaga acagtggctc aaaccactgg
    14941 cgaaccgctg gctggcaacg gcggcgtggg ggattattgc cctggtgtgg cttaccgtcc
    15001 gggtaatgaa gcgcctgcaa ctgcttgaaa aacagcaaaa acagcagcgc gaagaggcca
    15061 ttgatccgct cagtgtggag ctgaacgccc agcagcgtta ccttgaccgc tggctgctgc
    15121 gcctgcaacg ccatctcgac aaccgccgtt tcctgtggca gttaccgtgg tatatggtca
    15181 tcggcccggc gggcagtggc aaaacgacac tgctgcgcga ggggtttccg tcagacatta
    15241 tttatgcccc ggagggcgcg cgcggcacag aacaacgcct gtaccttacg ccccatgtcg
    15301 gtaaacaggc ggtgatcttt gatatcgacg gcacactctg cgctcccgct gatgcggata
    15361 tcctgcatcg ccgtctgtgg gaacatgccc tcggctggct gaaagaaaag cgcgcgcgcc
    15421 agccgctgaa cgggattatt ctgacactcg atttacccga tctgctgacc gcagacaaac
    15481 gccaccgcga gcatctgtta cagacgctgc gcagccgctt acaggatata cgccagcatc
    15541 ttcactgcca gttaccggtt tacgtggtac tgacccggct tgatttatta cagggttttg
    15601 ccgccctgtt ccagtccctg aacagacagg atcgcgatgc gattctgggc gtcacgttca
    15661 cccgccgtgc ccatgaaaat gatgactggc gaacagagtt gaatgctttc tggcagacat
    15721 gggtggatcg aatgaatctg gcgttgccgg atctgatggt cacccagact cacacccgcg
    15781 cgtctttatt cagtttttcc cgccagatgc agggaagccg tgaaccgctg gtgtcactgc
    15841 ttgagggtct gcttgacggt gaaaatatga acgtgatgct gcgtggtgtc tatctcacct
    15901 cttcgcttca gcgcgggcag atggatgata tattcaccca gtctgccgcc cgccagtacc
    15961 gactgggcaa taacccactg gcgtcctggc ctctggtgga caccgcgcct tatttcaccc
    16021 gcagcttgtt cccgcaggca ttactcgcag agcctaatct ggcaacggag agccgcgcct
    16081 ggctgatgcg ttcccgtcgc cgcctgacgg ttttctctgc caccggcggc gtggcggcac
    16141 tgctgctcat caccggctgg catcactatt acaacggtaa ctatcagtcc ggcatcaccg
    16201 tgcttaagca ggccaaagcc tttatggatg tgccgcctcc acagggggaa gatgactacg
    16261 gcaacctgca actgccgctg ctgaacccgg tacgcgatgc cacactggcc tatggcgact
    16321 ggggcgaccg cagccgtctg gccgatatgg gactgtacca gggacgacgt atcggacctt
    16381 atgtggaaca gacctatctg caactgctgg agcaacgtta cctgccctcg ctgtttaacg
    16441 ggctggttaa agcgatgaat gccgcgccgc cggagagtga agaaaaactc gcggtgctgc
    16501 gcgtgatgcg aatgctggag gacaaaagcg gacgtaacaa tgaggtggtg aagcagtata
    16561 tggcaaaacg ctggagcgaa aagttccacg gccagcgcga tatccaggca caactgatgt
    16621 cccatcttga ctacgcgctg gctcatactg actggcacgc agagcgtcag gcgggcgacg
    16681 gtgacgccat cagccgctgg acgccatatg acaagcccgt ggtatcagca cagaaagaac
    16741 tgagcaaact gcctgtctac cagcgggttt accagagcct gaaaacgcgg gcgctgggcg
    16801 ttcttcctgc cgacctcaat ctgcgtgacc aggtagggcc aacctttgac caggtgttta
    16861 catctgccga tgacaacaaa ctggttgttc cacagtttct tacccgttac ggcctgcaaa
    16921 gctattttgt aaaacagcgc gatgaactgg ttgaactgac ggcgatggat tcctgggtac
    16981 ttaaccttac ccgcagcgtg aaatacagtg acgccgaccg cgcggaaatc cagcgccagc
    17041 tcaccgagca gtatatcagc gactacaccg ccacctggcg ggccgggatg gacaatctga
    17101 atatccgcaa ttttgagtcc atcggacaac tgaccggggc gctggagcag gttatcagcg
    17161 gcgaccagcc tttgcagcgg gcgctgaccg tgctgcgtga caacacacag ccaggcgtct
    17221 tttctgaaaa actctctgcc aaagaacggg atgaggccct ggcagagccg gactaccagt
    17281 tactcacccg tctcgggcat gagttcgccc cggaaaacat taccctggca gtacagaaag
    17341 acaaagaaag cacgatgcag gccgtgtatc agcaactcac cgagttgcac cgctacctgc
    17401 tggcaatcca gaacgcgcct gtaccaggga aatcggcgct gaaagccgtg cagttacggc
    17461 ttgatcagaa cagcagcgat ccgatattcg ccacccgcca gatggcaaaa acgctgcctg
    17521 ctccgctcaa ccgctgggtt ggcagactga ctgaccaggc ctggcatgtg gtgatggtgg
    17581 aggctgtaca ttatatggaa gtggactggc gcgacagcgt ggtgaaaccg tttaacgagc
    17641 aactggcaaa taactatccg tttaatccgc gttctgcaca ggatgcctca ctggatgcct
    17701 tcgaacgctt ctttaaaccg gatggcatac tggatacctt ctaccagcag aacctgaagc
    17761 tgtttatcga taatgacctg agtctggagg atggcgataa caacgtcatt attcgcgaag
    17821 atattattgc gcaactggaa actgcgcaga aaatccgtga catcttcttc agcaaacaga
    17881 acggtctggg aacatccttt gccgtggaaa cggtatcgct ttcaggcaat aaacgccgca
    17941 gtgtactgaa ccttgacggt cagttagtcg attacagcca gggccgtaac tataccgccc
    18001 atctggtctg gcctaacaac atgcgcgaag gcaacgaaag taagctgacg ctcatcggca
    18061 ccagcggcaa cgcgccgcgc agtatcagct tcagcgggcc gtgggcgcag ttccgcctgt
    18121 tcggggccgg acaactgacc ggagtacagg atggcaactt taccgtgcgc tttagcgtgg
    18181 acggtggcgc gatgacctac cgtgtgcata ccgacacgga agataacccg ttcagcggtg
    18241 ggttgttcag ccagtttggt ctgtcagaca cactgtactg aggaggcagg gatgaatagt
    18301 aacgtactga cacaaactat cgttaccggc agtgacccgc gcgggctgcc ggaattcagc
    18361 gccatccgcg aggaaataaa caaagccagc cacccgtcac agcctgagct gaactggaaa
    18421 ctggtggagt cgctggcgct ggcgattttt aaagccaacg gtgtggattt acacaccgcc
    18481 acctactata cgcttgcccg gacacggaca cagggactgg cgggattctg cgaaggtgcg
    18541 gaactgctgg cggcaatggt aagccacgac tgggataagt tctggccgca gggcggcccg
    18601 gcgcgtactg aaatgctgga ctggtttaac tcccgaaccg gcaatattct gcgtcagcaa
    18661 atctcctttg cggaatccga cctaccactg atctaccgca cagagcgggc attgcagctt
    18721 atctgcgata agctccagca ggtggaactg aagcgcgttc cgcgcgtgga gaatctgctc
    18781 tattttatgc agaacacgcg taaacggctt gaaccccagc tgaagagtaa cactgagaac
    18841 gccgcacaga ccacggtcag aacgctgatt tatgccccgg aaacacaggc atcttccaca
    18901 ccagaagcgg tagtgcctcc cctgcccggc ctgcctgaga tgaaagtgga agtgcgcagt
    18961 ctgacagaga atcccccaca ggccagtgtg ataaagcaag gcagtacggt aagagggttt
    19021 atcgcaggga tcgcctgttc agtggctgtc gcctcagcat tgtggtggtg gcaggtctat
    19081 ccggtgcagc agcaactgtt acaggttaac gacaccgctc agggcgcagc aacggtgtgg
    19141 atggcctcac ctgaactcga aaactatgag cgcaggctgc aacaacttct tgatacctcc
    19201 ccggtacagc cgctggaaac cgggatgcag atgatgcgtg ttgccgacag tcgctggccg
    19261 gaaagcctgc aacagcaaca ggcctcgaca caatggaatg aggcactcaa aacccgcgca
    19321 cagagtagcc cgcagttgcg tggctggttg cagacccgcc aggacttaca tgcttttgca
    19381 gatctagtga tgcagcgcga gaaagaggga ctaacccttt cctatatcaa aaatgtcatc
    19441 tggcaggcgg agcggggact ggggcaggaa acacccgttg agtctctgtt gacgcagtac
    19501 caggatgccc gtgcgcagaa gcagaataca gatgcgctgg aaaaacaaat taatgagcga
    19561 ctcgaaggcg tgttaagccg ctggctgctg ctgaagaata atacgatacc gacgataaaa
    19621 aaagcattga atttcaataa cattcatgaa tacaaaggag ttttaaatgg cgaatttaat
    19681 ttatttaaca ctaaatggtg ataatcaagg gctcatttca agtggttgtt catcacaacc
    19741 atccattgga aataaagctc aaacagcaca cattgatcaa attatgattt atgaattcat
    19801 gcatggatta aatcgagatc aaaacgtcaa ccatcatcat ttgacaataa aaaaacctat
    19861 agataaagca tcacccttat taggcaaagc aatttgcgac aatgaattat tgacatgtga
    19921 tttttccttt tatcgtacaa ataaatttgg aattaatgaa ctgttctata aaatcaaatt
    19981 aactggagca aagataagcg acattcatgt ctctatatca catattgttg ttgataacag
    20041 cgttcaaccc gaagagtcag tttctttcag ttatgagtca attatatggg agcattgttc
    20101 cgctggcact agtgcataca gtttatggga agatcgtctt ttttaaaaac atatgggatt
    20161 attataatgg ctaacaatat ttt
//
LOCUS       DAZKIP010000076        19948 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZKIP010000076 DAZKIP010000000
VERSION     DAZKIP010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19948
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.076"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            50..574
                     /gene="lpfA1"
                     /locus_tag="ACHOUK_003057"
     CDS             50..574
                     /gene="lpfA1"
                     /locus_tag="ACHOUK_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA1"
                     /protein_id="HFP9898454.1"
                     /translation="MKKVVFALTALALTSGTVFAAESGDGTVKFTGEIVDSPCVLSVD
                     SQNQEVVLGQVQKSVFAAVGDKSPAKPFEIKLEDCDTTTMKKANVSFSGVGDADKSDL
                     ISVSTEAGAAKGVGIGIYDNSNTLVALNGGKASVDLSKGQTVLYFTANYVSTLATVTT
                     GYGNAQVDFNLSYE"
     gene            639..1325
                     /gene="lpfB"
                     /locus_tag="ACHOUK_003058"
     CDS             639..1325
                     /gene="lpfB"
                     /locus_tag="ACHOUK_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312457.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial biogenesis chaperone LpfB"
                     /protein_id="HFP9898455.1"
                     /translation="MKSKFVALALSLFLSQSVLAGGVGLSSTRVIYDGSKKEASLTVQ
                     NKSKTEEFLIQSWVDDAAGSKKTPFIITPPLFKLDPEKNNILRIVNITPGLPQDRESV
                     YWVNVKAIPSKSDDSENKNVLQIAVRTRIKLFYRPAGLKGDVKTAPNELRFTRNGNQL
                     RVDNPTVFNITFNQFFANDKEIEKAGMVPAKGALNITLPAGVGSVSKIKYNTINDFGS
                     SAEMITKNVD"
     gene            1354..3927
                     /gene="lpfC"
                     /locus_tag="ACHOUK_003059"
                     /pseudo
     CDS             1354..3927
                     /gene="lpfC"
                     /locus_tag="ACHOUK_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088424.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
     gene            3942..4997
                     /locus_tag="ACHOUK_003060"
     CDS             3942..4997
                     /locus_tag="ACHOUK_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9898456.1"
                     /translation="MKAAIALSLLGCVFGFSGKAFAGDAWGPCTPADGTTYHYNVDVD
                     VGIPDAAKNVAGTVLPDVLNWSNGQNVSLICECPDSYKNEKDTLVQGVSMLPPSGRTV
                     DSMKYYTLTEELEVATNIRISTSVYGFVPFKNQQALQTTGCNKVITTPYMGGAGLLSF
                     AITKPFIGDSVIPLTLIAELYASKTNKDYGTIPISSVSIQGRVTVTQDCEIKPGTVLD
                     VPFGEFPSSAFKNRQGQMPEGATEQEINLSFDCNNISDGIKVALRLEGATNADDPRAV
                     DMGNPDIGVLVKDSSGKILVPNDSSSTTLLNLSSLDSKTHRNAAIRLLALPISTTGKA
                     PKGGTFEGVTTIYLEME"
     gene            5002..5532
                     /gene="lpfE"
                     /locus_tag="ACHOUK_003061"
     CDS             5002..5532
                     /gene="lpfE"
                     /locus_tag="ACHOUK_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HFP9898457.1"
                     /translation="MKFKRLLHSGIASLSLVACGVNAATDLGPAGDIHFSITITTKAC
                     EMEKSDLEVDMGTMTLQKPAAVGTVLSKKDFTIELKECDGISKATVEMDSQSDSDDDS
                     MFALEAGGATGVALKIEDDKGTQQVPKGSSGTPIEWAIDGETTSLHYQASYVVVNTQA
                     TGGTANALVNFSITYE"
     gene            5787..7478
                     /gene="eptB"
                     /locus_tag="ACHOUK_003062"
     CDS             5787..7478
                     /gene="eptB"
                     /locus_tag="ACHOUK_003062"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312452.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFP9898458.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSASASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVNDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKAADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAAAN"
     gene            7570..7646
                     /locus_tag="ACHOUK_003063"
     tRNA            7570..7646
                     /locus_tag="ACHOUK_003063"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7604..7606,aa:Pro,seq:cgg)
     gene            8391..9998
                     /gene="dppA"
                     /locus_tag="ACHOUK_003064"
     CDS             8391..9998
                     /gene="dppA"
                     /locus_tag="ACHOUK_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFP9898459.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10149..11168
                     /gene="dppB"
                     /locus_tag="ACHOUK_003065"
     CDS             10149..11168
                     /gene="dppB"
                     /locus_tag="ACHOUK_003065"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFP9898460.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11178..12080
                     /gene="dppC"
                     /locus_tag="ACHOUK_003066"
     CDS             11178..12080
                     /gene="dppC"
                     /locus_tag="ACHOUK_003066"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFP9898461.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12091..13074
                     /gene="dppD"
                     /locus_tag="ACHOUK_003067"
     CDS             12091..13074
                     /gene="dppD"
                     /locus_tag="ACHOUK_003067"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFP9898462.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13071..14075
                     /gene="dppF"
                     /locus_tag="ACHOUK_003068"
     CDS             13071..14075
                     /gene="dppF"
                     /locus_tag="ACHOUK_003068"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFP9898463.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKIGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14105..15376)
                     /gene="yhjV"
                     /locus_tag="ACHOUK_003069"
     CDS             complement(14105..15376)
                     /gene="yhjV"
                     /locus_tag="ACHOUK_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFP9898464.1"
                     /translation="MQHNTLPKHDQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFTMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKTWLILLYGILLCLSPLLKLIE"
     gene            15852..15959
                     /gene="ldrD"
                     /locus_tag="ACHOUK_003070"
     CDS             15852..15959
                     /gene="ldrD"
                     /locus_tag="ACHOUK_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP9898465.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(16046..17725)
                     /gene="bcsG"
                     /locus_tag="ACHOUK_003071"
     CDS             complement(16046..17725)
                     /gene="bcsG"
                     /locus_tag="ACHOUK_003071"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFP9898466.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(17722..17913)
                     /gene="bcsF"
                     /locus_tag="ACHOUK_003072"
     CDS             complement(17722..17913)
                     /gene="bcsF"
                     /locus_tag="ACHOUK_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFP9898467.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(17910..19481)
                     /gene="bcsE"
                     /locus_tag="ACHOUK_003073"
     CDS             complement(17910..19481)
                     /gene="bcsE"
                     /locus_tag="ACHOUK_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFP9898468.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQYS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            19754..19942
                     /gene="bcsR"
                     /locus_tag="ACHOUK_003074"
     CDS             19754..19942
                     /gene="bcsR"
                     /locus_tag="ACHOUK_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFP9898469.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDILALKQAFSLPGIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
BASE COUNT         4995 a         4853 c         5260 g         4840 t
ORIGIN      
        1 aatacatcaa gattttcttt ttaatgtaat tttttaaatg gagtttttca tgaaaaaggt
       61 cgtttttgcc ttaaccgcac tggcgttaac ttctggcact gtattcgcag cagagtctgg
      121 tgacggcacg gttaaattta caggcgagat cgtggattca ccttgcgtac tgtccgttga
      181 ctctcagaac caggaagttg tgttgggtca ggtacaaaaa tctgtatttg ctgcggttgg
      241 tgacaaatcc ccggcaaaac ctttcgaaat caaactggaa gattgcgata ccaccacgat
      301 gaaaaaagcc aatgtgagct ttagtggtgt tggtgatgca gataaatctg atctgatttc
      361 tgttagcact gaagcgggcg cggccaaagg tgttggtatc ggtatttatg ataacagcaa
      421 taccctggtc gctcttaacg gtggtaaagc aagtgttgat ctgagtaaag gccagacggt
      481 tctctacttt accgctaact acgtttctac tctggcaacg gtaacgaccg gttacggcaa
      541 tgcgcaggtc gatttcaacc tgtcttacga gtaatcgtcc gttttacaaa tggcggcgta
      601 attgccgcca ttagtcgtca aagttcatgg atcgaatgat gaaaagtaaa tttgttgcgt
      661 tggctctctc tctgttcctt tcacagtcag ttctggcggg tggcgtcgga cttagctcta
      721 ctcgcgtaat ttatgatggc agtaagaaag aagcctcgct gaccgtacag aataaaagta
      781 agacggaaga gtttcttatc caatcctggg tggatgatgc cgcgggcagc aaaaaaacac
      841 cgtttattat tacgccccca ctattcaagc ttgatccaga aaagaacaat attttgcgta
      901 ttgtgaatat tacgcccggc ctgcctcagg atcgcgaatc ggtctactgg gttaatgtga
      961 aagccattcc atctaaaagt gacgacagcg aaaacaaaaa tgttttgcag attgccgttc
     1021 gcacccgaat caaacttttt tatcgcccgg caggtttaaa gggtgatgta aaaaccgcgc
     1081 caaatgaact gcgatttacg cgtaacggta atcagctccg cgttgataat ccgacagttt
     1141 ttaatattac ctttaatcaa ttttttgcga atgacaaaga aattgaaaaa gcaggcatgg
     1201 tacccgctaa aggggcatta aatatcacat tacccgccgg ggtaggtagc gtttctaaaa
     1261 ttaaatataa caccatcaat gactttggat cgtcagcgga aatgatcact aaaaatgttg
     1321 attgattaat atcttttcag gagaaatagc tcaatgtcac ggaagaccgt ttcacgtact
     1381 ttttcttcct tttctatctc tgtggtggcg gttgctgttg ccagcacgtt ttctgcacat
     1441 gccggaaagt ttaaccccaa attcctggag gacgttcagg gtgtgggcca gcatgtcgat
     1501 ttaacgatgt tcgaaaaagg ccaggaacag cagttacccg gcatctatcg tgtctctgtt
     1561 tacgtcaatg aacagagaat ggaaacccgc acactggaat ttaaagaggc cacagaagca
     1621 cagcgcaaag cgatggggga atcccttgtg ccttgcctta gccggacgca actggcagag
     1681 atgggcgtgc gcgttgaaag ctttccggcc ctcaatctgg tgtctgccga agcctgcgta
     1741 ccgtttgacg aaatcattcc cctggcctca agccatttcg acttcagtga acaaaagctg
     1801 gtattaagtt tccctcaggc ggcgatgcac caggtggcgc gcggcacggt gccggaatct
     1861 ttatgggacg aaggtatccc tgcgttgctg ctggattaca gttttagcgg gagcaacagt
     1921 gaatatgact cgacggggtc ttccagcagt tatgtcgatg ataatggcac tgttcatcat
     1981 gatgatggta aagatacgct aaagagcgac agctactacc tcaacttacg cagcgggctg
     2041 aaccttggcg cttggcggtt acggaattac agtacctgga gccattccgg tggcaaagcg
     2101 cagtgggata atatcggtac gtctttgagt agggccatca tccctttcaa agcgcaatta
     2161 acaatggggg atacggcgac cgcgggggac atttttgaca gcgttcagat gcgtggcgcg
     2221 atgttagcct ctgatgaaga gatgctgccc gacagccagc gaggctttgc acccatcgtt
     2281 cgagggatcg ccaaaagcaa cgccgaagtc agtattgaac aaaacggcta cgttatttat
     2341 cgcacctatg tgcagcctgg tgcctttgaa ataaacgatc tttaccccac tgcaaacagc
     2401 ggtgatctca cggtcattat taaagaagcg gatggcagtg aacaacgctt tatttagcca
     2461 ttctcatcgg tgccaatttt ccagcgtgaa ggccatctca aatatagctt tgccgcaggt
     2521 gaatatcagg ccgggaatta tgacagcgcc tcgccgcgtt tcgggcagct tgatctgatc
     2581 tacggtttac cgtgggggat gacggcctac ggcggcgtat taatctctaa taattacaat
     2641 gcatttacat tagggatagg gaaaaacttt ggttatatcg gggcgatttc cattgatgtg
     2701 acgcaggcta aaagcgaact gaataacgat cgcgatagcc agggacaatc ttatcgtttc
     2761 ttatattcca agagcttcga aagcggcacc gatttccgcc ttgcgggcta tcggtactct
     2821 accagcggtt tctatacctt ccaggaagcc accgatgtgc gcagtgacgc tgacagcgac
     2881 tataaccgtt atcacaagcg cagcgaaata cagggtaacc tgacgcagca attaggggcc
     2941 tatggctctg tttatttaaa tttaacgcag caggattact ggaacgacgc aggtaaacag
     3001 aacacggtat cggcgggtta caacggacgt attggcaagg tcagttacag tattgcatat
     3061 agctggaata aaagccctga atgggatgaa agcgatcgct tgtggtcttt caatatttcc
     3121 gttccactag gccgggcctg gagtaactat cgcgtcacga ccgaccagga tggtcgtacc
     3181 aatcaacagg ttggggtcag cggaacgctg cttgaggatc gcaacctgag ctacagtgtc
     3241 caggaaggct acgccagcaa cggtgtgggt aacagcggta acgctaacgt tggctatcag
     3301 ggtgggtccg gtaatgtcaa cgtaggctat agctacggga aagattaccg gcagctcaac
     3361 tacagcgttc gcggcggcgt gatagttcat agcgaaggcg tgacgctttc ccaaccgcta
     3421 ggcgaaacca tgacgctcat ctccgtaccc ggtgcgcgca atgcccgcgt ggtgaataac
     3481 ggcggcgttc aggttgactg gatgggtaac gcgatcgtgc cttatgccat gccgtatcgt
     3541 gaaaacgaaa tctcactgcg tagcgattcg ttgggtgacg atgttgacgt tgaaaatgcg
     3601 ttccagaaag tggtgccaac gcgtggagcg attgtcagag cgcgttttga tacccgcgtt
     3661 ggttaccgcg tattaatgac gctgcttcgt tccgcgggca gcccggtgcc ctttggagca
     3721 acggcaacgc taatcaccga taaacaaaac gaggtgagca gtatcgttgg tgaagaagga
     3781 cagctctata ttagcggaat gccagaggaa ggacgggtat tgattaaatg gggtaatgac
     3841 gcgtcgcagc aatgcgtggc gccttataaa ttatccctgg aattaaaaca gggcggaatt
     3901 attcctgttt cggccaattg ccagtaagca aggagagcga aatgaaagcg gcaatagcat
     3961 tatccttatt aggatgcgta ttcggatttt ctggcaaggc atttgccgga gatgcgtggg
     4021 ggccatgtac ccccgcggat ggcacgactt atcactataa tgtcgatgtg gatgttggta
     4081 tacctgatgc ggcaaaaaac gtcgcaggaa cggttttacc ggatgtcctt aactggtcta
     4141 atggacaaaa cgtatcgcta atttgtgaat gtcctgattc ttataagaat gaaaaagata
     4201 ctctagtgca gggggtgagt atgcttcccc cttctggccg tacggttgac agtatgaaat
     4261 attatacttt gacggaggaa ctggaggttg cgacgaacat tcgcatcagt accagtgtgt
     4321 atgggtttgt tcctttcaaa aaccagcagg cgctgcaaac aaccggatgc aacaaagtca
     4381 ttactacgcc ctatatgggc ggcgcaggtc tactttcctt tgctattact aaacccttta
     4441 tcggcgattc cgtcatccct ctgacgttaa ttgccgaact gtatgcctcg aaaacaaata
     4501 aagattacgg aacaataccc atatcttccg tatctattca ggggcgtgtc accgtaaccc
     4561 aggattgcga aattaaaccc ggcacggtgc tggatgtgcc atttggtgaa ttcccctcct
     4621 cggcgtttaa aaacaggcaa gggcaaatgc ctgaaggtgc gacagagcaa gaaattaacc
     4681 tcagcttcga ttgcaacaat atctctgatg gtatcaaggt tgcgttgcgc cttgaggggg
     4741 caaccaatgc tgacgatccc cgagcggtgg atatgggcaa ccccgatatt ggcgtactgg
     4801 tgaaagattc cagcggcaaa attttggtac cgaatgattc aagcagcacc acgttattaa
     4861 atttgagcag cctggattct aaaactcacc gcaatgccgc gattcgactg ctggctttac
     4921 cgattagcac gacgggtaaa gcgcctaaag gcgggacgtt tgaaggcgta acgaccatat
     4981 atcttgaaat ggagtgatgc catgaagttc aaacgattgc tgcatagcgg catcgccagt
     5041 ttgagtctgg ttgcctgcgg ggtgaatgcg gcgacggatc ttggcccggc aggggatatt
     5101 catttctcca tcactatcac cactaaagct tgcgagatgg aaaaaagcga tctcgaagtc
     5161 gatatgggaa caatgacgct gcaaaaacct gcggcagtcg gtacggtgtt gagcaagaaa
     5221 gatttcacca ttgaactcaa agagtgcgat gggatatcca aagcgaccgt tgagatggac
     5281 agtcagtcgg acagcgatga tgattccatg tttgcccttg aggctggtgg cgcaacgggt
     5341 gttgcgttga agatagagga cgataaagga acgcagcaag ttcccaaagg ctccagcgga
     5401 acgccgattg aatgggcgat tgatggcgaa accacgtcgc ttcactacca ggcgagttat
     5461 gtggtcgtca acactcaggc cactggtggc acagcgaatg cccttgtcaa tttttccatc
     5521 acctatgagt aagttactgg gccagcatcc tggcccaacc tatctctgaa tagtcgttaa
     5581 tccgttaaaa tggtctattc cggacttgca cttttgtaaa tttgtgcttc atgcacactc
     5641 tttccccaca ctttttccct ttgctgtggt ctacttattc gcgcgtgtag attttactta
     5701 tctgactacc tccgcacttt tcccctgctg ggcctgaaaa gccgctaagc agggtgttat
     5761 cacctgtttg tccagggttt gtttgcatga gatacatcaa atcgattaca cagcagaagc
     5821 tgagcttttt gcttgcaatc tatattggcc tttttatgaa tggcgcggtt ttttaccgcc
     5881 gcttcggcag ctatgcgcac gattttaccg tctggaaagg catttctgct gttgttgaac
     5941 tggccgccac cgtactggtg accttctttt tactacgtct tctttcgctg tttggccgcc
     6001 gcagctggcg tattctggca tcgctggttg tgctcttttc cgcaagtgcc agctattaca
     6061 tgaccttcct taatgtggtc attggttatg gcatcatcgc ttccgtcatg accaccgata
     6121 tcgacctgtc aaaagaagtt gttggtctga actttattct ctggttaatc gccgttagcg
     6181 cattgcctct tatccttatc tggaataacc gctgtcgcta caccttgctc cgacaactgc
     6241 gaaccccagg gcagcgtatt cgcagcctgg cggtcgtcgt actggcggga attatggttt
     6301 gggcaccgat tcgtttgctg gatatccagc agaagaaagt ggagagggcg accggcgttg
     6361 atttgccgag ttatggcggt gtcgtagcga actcttatct gccatcaaac tggctttctg
     6421 cgttggggct gtacgcctgg gcgcgggtcg atgaatcttc cgataataat tcattgctta
     6481 acccggcgaa gaaattcact tatcaggcac cgcaaaatgt taatgatact tatgtcgtgt
     6541 ttatcatcgg tgaaaccacg cgttgggacc atatgggcat tttcggctat gagcgtaata
     6601 ccacgccgaa gctggcccag gagaaaaatc tggcggcgtt ccgtggttac tcctgtgata
     6661 ccgcaaccaa actctctctg cgttgcatgt ttgtacgtca ggggggcgcg gaagataatc
     6721 cgcagcgcac attaaaagaa cagaacattt tcgcggtact gaagcagtta ggattcagtt
     6781 ctgacctcta cgctatgcag agcgaaatgt ggttctacag caatacgatg gcggacaata
     6841 ttgcttaccg tgaacagatt ggtgcggagc cacgtaaccg tggcaagccg gtagatgata
     6901 tgttgctggt agacgaaatg cagcaatcgc tggggcgcaa cccggatggt aagcatctaa
     6961 tcattctgca taccaaaggt tcgcacttta actacaccca gcgttacccg cgcagctttg
     7021 cgcagtggaa gccggaatgt attggtgttg atagcggatg taccaaagcg cagatgatca
     7081 actcctatga caactcggtg acctatgtgg atcactttat ctccagtgtg attgatcagg
     7141 ttcgcgataa gaaagcgatt gtgttctacg cagctgacca cggcgagtca attaatgaac
     7201 gcgagcacct gcacggcacg ccgcgtgaac tggcaccgcc ggagcagttc cgcgtaccga
     7261 tgatggtctg gatgtcagat aaatatctgg aaaatccggt caatgcgcag gcgtttgcgc
     7321 agctgaaaaa agcagccgac atgaaagtgc cacgccgtca cgtagagctg tacgacacca
     7381 tcatgggttg tcttggctat acttcaccgg atggtggaat taacgaaaac aacaactggt
     7441 gtcacatccc gcagacaaaa gaggcagcgg ctaactaaga ggcctgctga ctttctcgcc
     7501 gatcaaaagg cattttgcta ttaagggatt gacgagggcg tatctgcgca gtaagatgcg
     7561 ccccgcattc ggtgattggc gcagcctggt agcgcacttc gttcgggacg aaggggtcgg
     7621 aggttcgaat cctctatcac cgaccaaatt cgaaaagcct gctcaacgag caggcttttt
     7681 tgcatctgca gcccatgagg aagagaacct ccgggggcag ggaggttcga ctcgacgtag
     7741 gcctgataag acgcgtcagc gtcgcatcag gcaacggcac cgcacttatt gtcggatgcg
     7801 gcgtgaacgc cttattcgac ctacacgcca cacatttccc tgccaaattt gcgccccata
     7861 tctcactatc cattaacgga tttgtgacag aaaccattgt ctttttttat atttgcttaa
     7921 atcttttttt gcgttactta tggctggcgc tggcattttc cgctgtgcgt tttagcgttc
     7981 atggtattaa tcgctcagat atccaccttt tcctcagatt tttttcacta aatgcataaa
     8041 acttaatcac caaatgttgc taaatattaa gctgattgtt ctggtcatgt tggagtagca
     8101 caaatttctc tgctgcaaat agctgtttga agttttttta atctttgttt gtggattctc
     8161 accgttggtg taaatcccgg ttggctgtat tgacgttttc acattctgtt gacagattgt
     8221 aggtcacgag gggcatttta tggaggatcc gcactgttac actgatgtta attagtacgg
     8281 catccccacc tcataacgtt gacccgaccg ggcaaaaaac aaaaaaggtc aggcagcgac
     8341 aacccactgc aaagggttaa aacaacaaac atcacaattg gagcagaata atgcgtattt
     8401 ccttgaaaaa gtcagggatg ctgaagcttg gtctcagcct ggtggctatg accgtcgcag
     8461 caagtgttca ggctaaaact ctggtttatt gctcagaagg atctccggaa gggtttaacc
     8521 cgcagctgtt tacctccggt accacctatg acgcctcttc cgtaccgctt tataaccgcc
     8581 tagttgaatt taaaatcggc accaccgaag tgattccggg cctcgctgaa aagtgggaag
     8641 tcagcgaaga cggtaaaacc tataccttcc atctgcgtaa aggtgtgaag tggcacgaca
     8701 ataaagaatt caaaccgacg cgcgaaatga atgccgatga cgtggtgttc tcgttcgatc
     8761 gtcagaaaaa cgcgcaaaac ccgtaccata aagtttctgg cggcagctac gaatacttcg
     8821 aaggcatggg cttgccggag ctgatcagcg aagtgaaaaa ggtggacgac aacaccgttc
     8881 agtttgtgct gactcgcccg gaagcgccgt tcctcgctga cctggcaatg gacttcgcct
     8941 ctattctgtc aaaagaatat gctgatgcga tgatgaaagc cggtacaccg gaaaaactgg
     9001 acctcaaccc aatcggcacc ggtccgttcc agttacagca gtatcaaaaa gattcccgta
     9061 tccgctacaa agcgtttgat ggctactggg gcaccaaacc gcagatcgat acgctggttt
     9121 tctctattac ccctgacgct tccgtgcgtt acgctaaatt gcagaaaaat gaatgccagg
     9181 tgatgccgta cccgaacccg gcagatatcg cccgcatgaa gcaggataaa tctatcaacc
     9241 tgatggaaat gccggggctg aacgtcggtt atctctcgta taacgtgcag aaaaaaccac
     9301 tcgatgacgt gaaagttcgc caggctctga cctacgcggt gaacaaagac gcgatcatca
     9361 aagcggttta tcagggcgcg ggtgtatcag cgaaaaacct gatcccgcca accatgtggg
     9421 gctataacga cgacgttcag gactacacct acgatcctga aaaagcgaaa gccttgctga
     9481 aagaagcggg tctggaaaaa ggtttctcca tcgacctgtg ggcgatgccg gtacaacgtc
     9541 cgtataaccc gaacgctcgc cgcatggcgg agatgattca ggcagactgg gcgaaagtcg
     9601 gcgtgcaggc caaaattgtc acctacgaat ggggtgagta cctcaagcgt gcgaaagatg
     9661 gcgagcacca gacggtaatg atgggctgga ctggcgataa cggggatccg gataacttct
     9721 tcgccactct gttcagctgc gccgcctctg aacaaggctc caactactca aaatggtgct
     9781 acaaaccgtt tgaagatctg attcaaccgg cgcgtgctac cgacgaccat aacaaacgcg
     9841 ttgaactgta caagcaagcg caggtggtga tgcacgatca ggctccggca ctgatcatcg
     9901 ctcactccac cgtgtttgaa ccggtacgca aagaagttaa aggctatgtg gttgatccat
     9961 taggcaaaca tcacttcgaa aacgtctcta tcgaataatt aaaagccata cccggtggtg
    10021 ctgtgcctct gcgtgcagcg ccatccggca gcaatacttc attccctgcc cgacctgggc
    10081 gggaattgat ttgtgagcaa tacagacacg cagttccagg ctgcgggtca ctacagagaa
    10141 tccgggttat gttgcagttt attctccgac gtttgggact cgtcatcccc acgtttatcg
    10201 gtattaccct tctcacattt gcctttgtcc acatgatccc gggcgatcct gtgatgatca
    10261 tggcgggcga acgtgggatc tccccagagc gtcacgcgca gttgctggct gaactcggct
    10321 tagataaacc gatgtggcag cagtatctcc attacatttg gggcgttatg catggcgatc
    10381 taggcatttc aatgaaaagc cgaattccgg tatgggaaga gttcgtgccg cgctttcagg
    10441 ccacgctgga acttggcgtc tgcgcgatga tttttgcgac cgcggtcggc attccggttg
    10501 gtgtgctggc cgcggttaaa cgcggttcca ttttcgatca cacagcggtt ggcctggcgc
    10561 tgacaggtta ttcaatgcct atcttctggt ggggcatgat gctgatcatg ctggtttcgg
    10621 tgcactggaa cctgacaccc gtctccggtc gcgtgagcga tatggtgttc ctcgatgatt
    10681 ccaatccgtt aaccggtttt atgctgatcg acaccgccat ctggggtgaa gacggtaact
    10741 ttatcgatgc cgtcgcccat atgatcttgc ccgccattgt gctgggtact attccgctgg
    10801 cggtcattgt gcgtatgaca cgctcctcga tgctggaagt gctgggtgaa gattacatcc
    10861 gcaccgcgcg cgccaaaggg ttgacccgca tgcgggtgat tatcgtccat gcgctgcgta
    10921 acgcgatgct gccggtagtg accgttatcg gcctccaggt gggaacattg ctggcggggg
    10981 cgattctgac cgaaaccatc ttctcgtggc ccggtctggg gcgctggttg attgacgcac
    11041 tgcaacggcg cgattatccg gtggtgcagg gcggcgtatt gctggtggcg acgatgatta
    11101 tcctcgtcaa cctgctggtc gatctgctgt atggcgtggt gaacccgcgt attcgtcata
    11161 agaagtaagg ggtcatcatg tcacaggtta ctgaaaataa agtgattagc gcgccggtac
    11221 cgatgacccc tttacaggag ttctggcact attttaaacg caacaaaggc gcggtcgtcg
    11281 ggctggttta cgtcgtcatc gtgctgttta tcgcgatctt tgccaactgg attgctccct
    11341 ataacccggc ggaacagttc cgcgatgcac tgctcgcccc gccagcctgg caggaaggcg
    11401 gcagcatggc gcacttgctg ggtaccgatg atgtaggccg tgatgtgctg tcgcggctga
    11461 tgtacggcgc gcgcttgtcg ctgctggttg gctgtctggt ggtggtgtta tcgctgatta
    11521 tgggcgttat tctcggcctg atcgccggtt acttcggcgg cctggtcgat aacatcatta
    11581 tgcgcgtggt cgatatcatg ctggcgctgc caagtctgct gttggcactg gtgctggtgg
    11641 cgattttcgg cccgtcgatt ggtaacgccg cgctggcgct gacctttgtt gccttgccgc
    11701 actatgtgcg cttaacccgc gccgccgtgc tggtggaggt gaatcgcgat tacgtcaccg
    11761 cttctcgcgt ggcaggagcc ggggcgatgc gccagatgtt tattaacatc ttcccgaact
    11821 gccttgcacc gctgattgtt caggcgtcgc tcggtttctc taacgccatt ctcgatatgg
    11881 ctgctcttgg tttcctcggc atgggggcgc agccgccaac gccggagtgg ggcaccatgc
    11941 tctccgacgt gttgcagttc gcgcaaagcg cctggtgggt cgtgaccttc ccgggtctgg
    12001 cgatcctgct gacggtgctg gcatttaacc tgatgggtga cggtctgcgt gacgcgctcg
    12061 atcccaaact gaagcagtaa gaggttcgag atggcgttat taaatgtaga taaattatcg
    12121 gtgcatttcg gcgacgaaag cgcaccgttc cgcgccgtag accgcattag ctacagcgta
    12181 aaacagggcg aagtagtcgg gattgtgggt gagtctggct ccggtaagtc ggtcagttcg
    12241 ctggcgatta tggggctgat tgattatccg ggccgcgtga tggcggaaaa gctggagttt
    12301 aacggtcagg atttgcagcg tatctcggaa aaagagcgcc gcaacctggt gggtgccgaa
    12361 gtggcgatga tcttccagga cccgatgacc agccttaacc cgtgctacac cgtgggtttc
    12421 cagattatgg aagcgattaa ggtgcatcag ggcggtaata agagcactcg tcgtcagcgg
    12481 gcgattgact tgttgaatca ggtcggtatt cccgatccgg cttcgcgtct ggatgtttac
    12541 ccgcatcagc tttccggcgg catgagccag cgcgtgatga ttgcgatggc cattgcctgt
    12601 cggccaaaac tgctgattgc cgatgaacca accactgcgc tggacgtgac cattcaggcg
    12661 caaatcattg aactactgct ggagctacag cagaaagaga acatggcgct ggtgttaatt
    12721 acccatgacc tggcgctggt ggcggaagcg gcacataaaa tcatcgtgat gtatgccggt
    12781 caggtggtgg aaacgggcga tgcgcacgcc atcttccatg cgccgcgtca cccgtatact
    12841 caggcattgc tgcgtgcgct gccggaattt gctcaggaca aagaacgtct ggcatcgttg
    12901 cctggcgttg ttcccggcaa gtacgaccgc ccgaacggct gcttgcttaa cccgcgctgc
    12961 ccctatgcca ctgacagatg tcgcgctgaa gaaccggcgc tgaatatgct cgctgacggg
    13021 cgtcagtcca aatgccatta cccacttgat gatgccggga ggccgacact atgagtacgc
    13081 aagaggccac ctcgcaacaa ccgctgttgc aggctatcga cctgaaaaaa cattatccgg
    13141 tgaagaaagg tatgttcgct ccggaacgtc tggtgaaggc gctggacggc gtttcgttta
    13201 accttgaacg tggcaaaacg ctggcggtag taggtgaatc tggctgcggt aaatcgaccc
    13261 tcggtcggtt gctgacgatg attgaaacgc ccaccggcgg cgagctgtat taccaggggc
    13321 aggatctgct taagcacgat ccgcaggcgc agaagctgcg tcggcagaaa atccagattg
    13381 tcttccagaa cccttacggt tcgctgaatc cgcgtaaaaa aatcgggcaa attcttgaag
    13441 agccgctgct gatcaacacc agcttaagca aagaacagcg tcgggaaaaa gccctgtcga
    13501 tgatggcgaa agtcggcctg aaaaccgagc actatgaccg ctatccgcat atgttctccg
    13561 gcggtcagcg tcagcgtatc gccattgccc gaggtctgat gctcgacccg gacgtggtga
    13621 ttgccgatga accggtctcc gcgctggacg tctcggtgcg tgcacaggtg ctcaacctga
    13681 tgatggattt gcagcaggaa ctggggcttt cttatgtctt catctcccac gacctgtcgg
    13741 tggtggagca cattgctgat gaagtgatgg tgatgtacct gggccgctgc gtggagaagg
    13801 gaacgaaaga ccaaatcttc aataacccgc gccatccgta cactcaggcg ctactttccg
    13861 cgacgccgcg cctgaatccg gacgatcgcc gcgagcgcat caaactcacc ggtgaactgc
    13921 caagcccgct gaatccaccg ccgggctgcg cgtttaacgc ccgctgtcgt cggcgcttcg
    13981 gcccctgcac ccagttgcag ccgcagctaa aagactacgg cggtcaactg gtagcttgtt
    14041 ttgctgttga tcaggatgaa aatccgcagc gttaactcac aaaaccgggg ccatgcgctc
    14101 cggtttactc aatcagcttc aacagtggcg acaagcatag caaaatgccg taaagcagaa
    14161 tcagccaggt tttaaatccg cgaagttttt ccagttgtgc gactttatag atcaggaaaa
    14221 acggaatgag gcacgacaca atgccatata acgggcttcc caactgaaag aacaccagca
    14281 ccgatacacg aaacgaaacc caaatcgtca acgtaatgac gataaaagcg cagatcgcca
    14341 gagtcagcac gcgtgagtta attttcttgg tatcaataat tcggcttaac agattgagaa
    14401 taatgccttt aatggcctcg tggaaaccga gataaatgcc aaagaatgcg gtcagtacgg
    14461 caaagatatt cagcaccgta gaggtgatat gaatgatatg cccagggatc acctgcgcgg
    14521 ccagtgccag tgccgagata ttttgttcaa aggcagaaac ggcttcttcg tggctaattg
    14581 agaaggtaaa cgaaaaggca aaaaacagta tcaccgcgat gagcgtgata taactaattc
    14641 ggtgggtacg cagcgcgagc cgggttgcca gtactttatc cgcttcccgt ttacgatagg
    14701 caatattcat tgggttaagt acctgaataa atactgcaga aaagaagcaa aatggaatgg
    14761 taagcaggac atcgcgaaaa aagacggagg cttgcgggaa ggcggttata ttggcgaaat
    14821 tccagtgcgg gatcatcgta aaaccgaaca cgacaataat ccctactttg accaccacca
    14881 ttggcccaga aatcttaaac agtaatcgtt caccaccaga cgcaatcgct accagtacgg
    14941 cgaaaatagc gactttataa agtagagatt gtgaaagatc ggcatcggtt aaaccgaagg
    15001 ttttcaggta cgaggcgctg tcgaaaacca cggagagaga gtagataaaa atcccgtgga
    15061 taatcatcaa aaagtagata accccgagga aaattcccca gttcttcccc aggtaatgac
    15121 tgataatatc ggtgtagtca ttacaggaat cgctttcaga aagggttttt aaataaatgt
    15181 cctgcactac ccaggtggca ggataagcaa tgatcgctgc ggtaataaat acccaaatgc
    15241 ccttcaagcc aatttgtact ggcatcagca cggttccggc accaatcgcc atgcctatgc
    15301 ataataaaac ccagccgaag tcgtagcgtg taaacggcaa tttctgatcg tgtttcggta
    15361 gtgtgttgtg ctgcataaag tcctgtccag aaagaagtcg gcctcgcaaa aatgaataaa
    15421 cccagtttct cttgcgcaac aggtggagaa gtcgaatacg cagtatgtaa aaaatgaggc
    15481 aacagattca ctggggaggt cgcgaggagg cgcaattcta caaaattgcg catttcacta
    15541 ccagtctgga gtcagcccgg atatgaactg caactattgc aaattaagaa attaagctgg
    15601 aaggcatcac gcaatgagga agattgccga gtgctgacag taaaaagcaa tcgtaaaatt
    15661 agcggcaagc cggaagggtt ccggtgaggc gcaatgttgc gggggcttaa tccctggtgg
    15721 cattggttgc tggagagaga aaacccccgc acgttgcagg tatgcacctg acaacaccac
    15781 gggggctaat cttgaaccta gaccactcaa gaatagccgc gaaacgttgt cattacaaca
    15841 caggcggcta tatgacgctc gcagagctgg gcatggcctt ctggcatgat ttagcggctc
    15901 cggtcattgc tggcattctt gccagtatga tcgtgaactg gctgaacaag cggaagtaac
    15961 gtgtcatgcg ggcgtcaggc tgccgtaatg gcaatttgcg cccggaccag accgcagggg
    16021 ggaaactctg cggccttttt cgtgcttact gcgggtaagg cacccagtcg ccgccgttca
    16081 ggcgaacgta cggtttatcc tggtattgaa taactactgc atttgagttc tctgagaccg
    16141 gtgctgtttg tggcaaccca ctggtgagtt ttttccagtc aacattgtct tcggtgaaaa
    16201 tcttgccatc aagaacgcga accaccagat cggagatagc caggaagctg ctcggttggt
    16261 cgatgacaat cggtgccccc tgatgtggtg ccttcatgcc gaagaatttc accccaacgg
    16321 ggacgtcggt gatagacggg ctagggatat cacgtaggcc agatacctgc attctgtcgc
    16381 ccttcagcgc gccgccgtgt tccggcacca cgaccaccat cactttacga cccgatttct
    16441 ccagttcagt aaagaaggcg tccagttcat caaagaattt ctgcgcccgc gctttgtaat
    16501 ccgctgtttt gctgacgccc ggataatggt tgccgtcatg cagtggaagc gtgttgtaga
    16561 acgtggcact acggctgttt ttatcttttt cggtaacgtc cagccagcgg ttaagcacgg
    16621 cggtatcgtc ataaaccggc gaaccatcaa agcccagcaa aataaccggc agatttgttt
    16681 gatccatcaa ttcagtctgc atgccgccat tttcgcgaac ttctttcaaa aaaccgccga
    16741 actggccgtt atgtcccatc atcaggtgct gggtaaagcc cagtttcgac aggttatcaa
    16801 acagatagca gtcgttattt gccggttgat acagattagt gtgcgaagtc tgcccgcagc
    16861 tggcgcgcag taaacggatc gccgccgggc cactgtagga ggtggcggag ttaaagttct
    16921 tgaactcaat atcgaaatgc gaccacagtg gatgcgacat caacccagcg gcttctatat
    16981 ccgaccagga aagcgaacag atgttaatca ccagtagttc aaatggctga gcatcagcgg
    17041 gcagcgaaga cgggaaggtc gatttacgtt tcgcctccgc gttatagaaa ttattcagcc
    17101 aggcgttaag gttcgccgtt gttggcggtg cagtttgtgc gggcatatca cccactaccg
    17161 gtgcgccacc cgtcgccgca acggttgccg ctgcgttacc acccgtcgtt gttacagtgg
    17221 tcgtcggttg tccggctggc cacaaggaga aacttggtcc cgccagggta agtacgttca
    17281 gccatagcag tatggcaacc acaaaaacgg taatgcgaat ccattgtgac aggaataacc
    17341 aggccactaa taaaacaaaa atggccccaa tcatctgcca gttaataaag cgtgtgacaa
    17401 ggtcgattaa ataatcggta ctgaaccccg ccacctgcga accctggctc attatgcttt
    17461 ccgggccagg caaccaggtg tcatgccaga acaaagcaaa gccgatcggc agggcaatcc
    17521 agtggcgcaa gcgatgcagg ctgtagcgcg gaatgggcat cagcagaaac gcggcaaaca
    17581 ccaaattgag gagcggatgg aagttaagat atcccgccca caacaggccg aacttaacca
    17641 gaaaatagaa gttccagccg gaaaggccgc gccagtattg ccagagggaa gaaggcatgg
    17701 cggtattttg cgtaaattga gtcatttttt ggttgccctg gctttttccg tgcggcgtaa
    17761 cgtcccagcc ggtttaacat aacgaggttt agcaaagaac aaacgtaagg tgtcgcgaat
    17821 gcgtcgcaaa gagtgccgcg ccagatagcc cagcgggaaa aatatcagtg cgcaaacgac
    17881 aataatttca atgatatcgc tgatggtcat catgatgagc gctccacagc atcatctaac
    17941 agtcgcatgg gttctggtat tcgtcgccag tggcgaccat cgtgctcggc attgatgacc
    18001 ggtttagaac tttgcgttaa aggcagcggc atgccccatt gttctggggc aagcaggcgc
    18061 atctgcacca gctcggcact gatttgatca tcttcaaacc agaccatacg gtttgagaaa
    18121 atgtcgccag taggcaatgg gaaaatatga ttcaacgcgg tatccagatc gttaatccga
    18181 cagaatgaga gaaacagcac cagccgatta ccgccaatgg tcatgatatc gccggtacgg
    18241 ttagggcgac acagcgtcag ggcttgttca acgcggatac ccggtaccgg acgtagggca
    18301 accagaacgc ctttaccgtg ggcaggtaat agagggttat tcatcatgtt gttgacggca
    18361 ttacagaaca catcccactt ctggaaacca cgcagtttga gcggctgggt cattgacagc
    18421 aaggtagtga tatcttccgg cacatagcga ctaaacttct gcccttgcac gctttcgatc
    18481 atcgtcagac aacgggagag tggcgcattc cacggaataa ccatatttgc accgcaggcc
    18541 aataacaaac gttcatcggt ggcgcgtagg ctagcggtat tttcccgcac gaggattttc
    18601 atcgcactac cgcgctggcg acgcagggta tgaatgctgc gggccagtgg ctcgatttgc
    18661 gcattttgct gtaaagaaaa gaccaccgtc gccgcctgag cggtacgggc ttcattgaac
    18721 aggacttcgt tattgttgaa cagttgccag tgttccgata gcggcggcgc accttccagt
    18781 acagcaacat tactcagaat gcgtttttcg tcgctgcgtg gttggatctc cgcctcttcg
    18841 ctttgaacta atgtccagat accattttgt tgctgaacgc taagctgctg acgggcgctg
    18901 accccttttt cgttgcacca gaaggcaata tccagcaaat gttggtcgcc ctgaaaacgc
    18961 aaactggcaa gaccaaaaag tgaacggtat tcctcaagca acaatgaaaa ttgtttatcg
    19021 ttattatttc cgggattaat taccaaaagc gaacaatggt ttaacctgct ccatttattc
    19081 attttatcca accatgagcg cagccgctcg gcaggaatgt tttgccatgc gttatttgcg
    19141 caaacaagaa taaaaaggta attatgggga tcaatagaat actgcaaatc acgggccaaa
    19201 tagtatagac ctttttcatg atttagcatt gaaaataatt ttattttttc cggcccttga
    19261 gaatcatcta attgaaagat tttcgccgga tcgctgtcca tgctaatgac cgcgacgtgt
    19321 gcggtttcag cctgggatgc aattgtttga ttcgccagac tgatagcatc ttcatggcga
    19381 tcgacgttaa accaccagac gccgcctgct ggcatatgtc gcagctcatc ccataatgat
    19441 gagataccga tagagaatac agggtccaca atgtccctca tgtcaccatt ttgtctgatt
    19501 atcagtttac tagcgaaagc taagaaataa acctaccatt gaaattaaaa cttatcagat
    19561 ttagcatgtg aatcaattta gcaggtcaca agacgtgtga ttttagtctt ttcgttttta
    19621 actttttctg ttgcgtaacg gaaagtcaaa aagtgagcaa attcccgtct cgccgtaaac
    19681 aataaaccgg cgaaatgctc acggttagaa ttgtctcatg aacggtacgg ttatttcata
    19741 gggatcaagc aaaatgaata acaatgaacc agatactctg cctgatcccg cgataggcta
    19801 tatcttccag aatgatattt tggcgttaaa gcaggcattt tcactgcctg gtattgatta
    19861 tgccgatatt tcccaacgcg aacagttggc cgcggcatta aaacgctggc cgttgctggc
    19921 agagtttgcg caacaaaagt aggggatt
//
LOCUS       DAZKIP010000077        19862 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZKIP010000077 DAZKIP010000000
VERSION     DAZKIP010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19862
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.077"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            170..433
                     /gene="ykgM"
                     /locus_tag="ACHOUK_003075"
     CDS             170..433
                     /gene="ykgM"
                     /locus_tag="ACHOUK_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFP9898470.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            433..573
                     /gene="ykgO"
                     /locus_tag="ACHOUK_003076"
     CDS             433..573
                     /gene="ykgO"
                     /locus_tag="ACHOUK_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFP9898471.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(643..834)
                     /locus_tag="ACHOUK_003077"
     CDS             complement(643..834)
                     /locus_tag="ACHOUK_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9898472.1"
                     /translation="MQAASQREETEWRVQSKRGLMPAYRGEAGQQVNINIMEYSERNV
                     RQLTLNEQEDTSPGKLMLV"
     gene            1659..2201
                     /gene="ecpR"
                     /locus_tag="ACHOUK_003078"
                     /gene_synonym="matA"
     CDS             1659..2201
                     /gene="ecpR"
                     /locus_tag="ACHOUK_003078"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HFP9898473.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLTEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMLPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKLYKLVQ"
     gene            2276..2863
                     /gene="ecpA"
                     /locus_tag="ACHOUK_003079"
                     /gene_synonym="matB"
     CDS             2276..2863
                     /gene="ecpA"
                     /locus_tag="ACHOUK_003079"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HFP9898474.1"
                     /translation="MKKKVLAIALVTVFTGMGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            2921..3589
                     /gene="ecpB"
                     /locus_tag="ACHOUK_003080"
                     /gene_synonym="yagY"
     CDS             2921..3589
                     /gene="ecpB"
                     /locus_tag="ACHOUK_003080"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HFP9898475.1"
                     /translation="MKKHLLLLALLLSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGTLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            3615..6140
                     /gene="ecpC"
                     /locus_tag="ACHOUK_003081"
                     /gene_synonym="yagX"
     CDS             3615..6140
                     /gene="ecpC"
                     /locus_tag="ACHOUK_003081"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HFP9898476.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNFGIYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGTLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSSIGSI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGTFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYVNTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            6130..7773
                     /gene="ecpD"
                     /locus_tag="ACHOUK_003082"
                     /pseudo
     CDS             6130..7773
                     /gene="ecpD"
                     /locus_tag="ACHOUK_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308347.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
     gene            7742..8452
                     /gene="ecpE"
                     /locus_tag="ACHOUK_003083"
     CDS             7742..8452
                     /gene="ecpE"
                     /locus_tag="ACHOUK_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HFP9898477.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPQDNRERYYRVSFREVPTRNLTKRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDQVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPEDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            8765..9094
                     /gene="ykgJ"
                     /locus_tag="ACHOUK_003084"
     CDS             8765..9094
                     /gene="ykgJ"
                     /locus_tag="ACHOUK_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFP9898478.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(9342..9956)
                     /gene="yagU"
                     /locus_tag="ACHOUK_003085"
     CDS             complement(9342..9956)
                     /gene="yagU"
                     /locus_tag="ACHOUK_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFP9898479.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            10374..11063
                     /gene="paoA"
                     /locus_tag="ACHOUK_003086"
     CDS             10374..11063
                     /gene="paoA"
                     /locus_tag="ACHOUK_003086"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047770 - carboxylate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit PaoA"
                     /protein_id="HFP9898480.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAAAAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAVLKEIQDGIPSHVTVDLVSPPERTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            11060..12016
                     /gene="paoB"
                     /locus_tag="ACHOUK_003087"
     CDS             11060..12016
                     /gene="paoB"
                     /locus_tag="ACHOUK_003087"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HFP9898481.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLVAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYAFALVSVAAIIQPDGSGRVALGGVAHKPWRIEAADAQLS
                     QGAQAVYDALFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            12013..14211
                     /gene="paoC"
                     /locus_tag="ACHOUK_003088"
     CDS             12013..14211
                     /gene="paoC"
                     /locus_tag="ACHOUK_003088"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HFP9898482.1"
                     /translation="MKFDKPAGENPIDQLKVVGRPHDRIDGPLKTTGTARYAYEWHEE
                     SPNAAYGYIVGSAIAKGRLTALDTDAAQKAPGVLPVITASNAGALSKGDKNTARLLGG
                     PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVSQPPVDTPDK
                     NVGDFDGAFSSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKVMLPRPSIP
                     NNTTHRPATLQHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLYAGANRHT
                     GLRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDTQIDPADP
                     TRRFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNLLEKSGAR
                     VHLEPNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFPVSAGSGG
                     QWGANTSTSGVYAACVKLREMIASAVGFDPEQSQFADGKITNGTRSAILHEATAGGRL
                     TAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRILNPKTARS
                     QVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFLDDTDPIS
                     SPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
     gene            14221..15177
                     /gene="paoD"
                     /locus_tag="ACHOUK_003089"
     CDS             14221..15177
                     /gene="paoD"
                     /locus_tag="ACHOUK_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HFP9898483.1"
                     /translation="MSYPLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAVLV
                     TLVEIRGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGCDREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNRELPVLQAAREAKPFYLGALGSYRTHTLRLQKLHELGWSREETAQ
                     IRAPVGIFPKARNAHTLALSVLAEVASVRLHQEEDSCLPPSS"
     gene            complement(15356..16483)
                     /locus_tag="ACHOUK_003090"
     CDS             complement(15356..16483)
                     /locus_tag="ACHOUK_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9898484.1"
                     /translation="MTLCVFVLIASEFMPVSLLTPIARDLGVTEGLAGRGIAISGALA
                     VLTSLTLSTLAGKMNRKFLLLGMTVLMAVSGLIIALATSYLMYMVGRAMIGVAIGGFW
                     SMSAATAIRLVPQHQVTRALAIFNAGNALATVVAAPLGSYLGATVGWRGAFLCLVPMA
                     VVAFIWQCISLPSMDANKSHTSCGAVFRLFSRRMVVVGMIACGLFFMGQFALFTYVRP
                     FLESVTRVNSLGLSLILLIIGVAGFIGTLVVSIFLNRKFYPTLMAIPGLMAVIAIGLM
                     LTGHHVWMVSLLLGLWGMLATAAPTGWWTWIARTLPDNAEAGGGLMVAVIQLSIALGS
                     TAGGIVFDRLGWQSTFAMSSVLLLCAGVLTFVTARQKAGAL"
     gene            complement(16625..17683)
                     /locus_tag="ACHOUK_003091"
     CDS             complement(16625..17683)
                     /locus_tag="ACHOUK_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9898485.1"
                     /translation="MSYADSTNPNAPVSMTDKWDKTFAESQKVDHRKVSFPNRYGITL
                     VGDLYLPKDRGDRKLAAIAVSGPFGAVKEQSSGLYAQTLAERGFVTLAFDPSYTGESG
                     GYPRNVASPDINTEDFSAAVDFLGLQKEVDRNRIGLLGICGWGGMALNDAAMDTRVKA
                     VATSVMYDMSRAMGHGVGDGEDRYSTADRRAVLQYLNEQRWKDAQSGTFAHGGHDINV
                     DSNGKVSAGERILPETLPANPHPVLKEFFDYYRMPRGFHERSVNSTGAWTATMPLSFM
                     NMPLLSYASEITIPTLIVTGEKAHSRYFAEDAYKAVGSKDKELVVVPGANHVDLYDNV
                     AGKIPFAKFEQFFQTKLK"
     gene            17929..18831
                     /locus_tag="ACHOUK_003092"
     CDS             17929..18831
                     /locus_tag="ACHOUK_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9898486.1"
                     /translation="MKGNVSMAKRENYNELYLFMQVVREGSFTAAAKRLGLAQSGVSR
                     SVRELEERLGVQLLVRTTRKLSLTQAGEQLYQKTASGFEMLDLGLATLAHYRETPSGT
                     VRINASQHAIDKCLLPKLAVFKQRYPDIQLELINESRFVDIIEERFDAGVRLGPEVSQ
                     GMVAVRITPDMEMAIVGTPEHFRRYGFPQTPADLKAHPCIAYQFADGSVYQWELNQDD
                     KKITHQPEGQWAFSDSYMEAEAARLGLGLAYVPVELVADDLEHGKLIRVLQRYSLRME
                     GLFLYYPHRNVSPALRMVIDTLKI"
     gene            19068..19130
                     /locus_tag="ACHOUK_003093"
     CDS             19068..19130
                     /locus_tag="ACHOUK_003093"
                     /inference="COORDINATES: protein motif:HMM:NF041474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9898487.1"
                     /translation="MNTVVFVATVEYAYLSLHFY"
     gene            complement(19534..19812)
                     /locus_tag="ACHOUK_003094"
     CDS             complement(19534..19812)
                     /locus_tag="ACHOUK_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9898488.1"
                     /translation="MSLYIKLILSIVREISVNTICSLIVVVALSLLSFSSVAKTITAV
                     GSTINSTEKEISLQAEKQGKSYKILGAFFKNRVYMIAKLTPVSKNDAS"
BASE COUNT         5065 a         5201 c         5066 g         4530 t
ORIGIN      
        1 atcactcatc caggcgttta tgggagcgtc gccaatggct gttaacaatg ccagacttcc
       61 ccgttgagga aattccacat accactaatg gtcacaggga ttgggtgttg aaatgatccg
      121 gatgagcatg tatctttgtg gttatgttat aacataacag gtaaaaatga tgaagcccaa
      181 tatccatcct gagtatcgta ctgtggtgtt ccacgacacc agtgttgatg agtactttaa
      241 aatcggctcg actatcaaaa cagaccgtga gattgagctg gatggcgtaa cgtatccgta
      301 cgtaacaatt gatgtctctt cgaaatcgca cccgttctat acagggaagc tgagaacagt
      361 ggcatcagaa ggaaatgttg cacgattcac ccaacgtttt ggtcgttttg ttagcacgaa
      421 aaagggggcg tgatgaaagt ccttaactct ctgcgtaccg caaaagaacg ccatccagac
      481 tgtcagattg tgaagcgaaa aggacggcta tatgtgattt gtaaatctaa tccacgcttt
      541 aaggccgttc agggtcgtaa gaaaaaacgt tgatttaaaa ttcgacgaat taacgatatt
      601 tgtctgatta ataatcagac cggattaatg ttggtgtatt tattacacca gcattaattt
      661 tcctggggat gtatcttcct gttcatttaa ggtcaactgc ctgacgtttc tctccgaata
      721 ttccattata ttaatgttga cttgttgacc agcttcgccc ctgtatgctg gcatcaaccc
      781 tcttttagac tgaacacgcc actcagtttc ctccctttgc gacgcagcct gcatttacac
      841 tcaaactgtt aagatgataa atgtggtaaa tctgttggta ctgacataaa aacgtttacg
      901 ccacaggaac ggcctgatcc accggtaacc ccgtcgccga cgttcgagtg ccagttagag
      961 taacgcgcac agatgactga atgcagtgcc ctggcaaaga ggccatcgtt ccggtgacaa
     1021 ctggcagtct tcgtttaact tcacttaatc tggctcttag ggggcttacc ggacagatga
     1081 cgtacttaca cctgtttaat ttttcatcac ttattgggat gaacacccat aaccattttg
     1141 tgcggcatgt taatccatta aaacacctta ctgattggca aatcatcttt aattatgact
     1201 catgatagtt tcatattcta tttcttatca tttagacttg tttttttact agtccattac
     1261 acaacacatt aagactattc ctaacacctc agggcaaagt tcctggctaa tataaaatgc
     1321 aagtaagaat tgaacgttat attgccaata accttatgaa actgaatgtc tttttcttct
     1381 tatcaaaaaa gcaatatttt cattttttgt aaatattgac ttaaccatgg aattcatttt
     1441 ctgttcacat attgacactc atcaggaaaa aaacataaat ttaaacctaa tcgaaataat
     1501 taaaaactta atctcgttta acctatattg atatgtgcta cgtatcttat ttacttccga
     1561 tttactaaag aaactgaatg tacctgtaaa aattacaggt ttggaaagta gtgacatggc
     1621 aaaatgatta cagcagggac tatgaggttg aaaaccatat ggaatgtcaa aaccgttctg
     1681 ataaatacat ctggtctccc catgacgcct acttctataa aggactatct gaactgattg
     1741 tggatatcga cagattaatt tatctatcgc tggagaaaat cagaaaagat ttcgtgttta
     1801 tcaatctcaa tacggattct ttaactgaat ttataaaccg tgataatgag tggttatccg
     1861 cggtaaaggg gaaacaggtc gtattgattg cggccagaaa gtcagaagcc ttagcaaatt
     1921 attggtatta caacagcaat attaggggcg tggtatacgc tggactgagt cgtgatatta
     1981 gaaaagaact ggcctatgtg attaatggca ggttcctgag aaaagatatt aagaaagata
     2041 aaatcactga ccgggaaatg gaaattatcc gcatgacggc tcagggaatg ctgcctaaat
     2101 cgattgccag aattgaaaat tgtagtgtaa agacagtgta tacccatcgg cgtaatgcag
     2161 aggccaagct gtactcaaaa ttatataagt tggttcagta aactccaggc aagttagttt
     2221 taaaaaatga ctcactggga catcacgtcc tcaattcaac tcgggaagaa atacaatgaa
     2281 aaaaaaggtt ctggcaatag ctctggtaac ggtgtttacc ggcatgggtg tggcgcaggc
     2341 tgctgacgta acagctcagg ctgtagcgac ctggtcggca acagccaaaa aagacaccac
     2401 cagtaagctg gttgtgacgc cactcggtag cctggcgttc cagtatgccg aaggcattaa
     2461 aggttttaac tcacagaaag gtctatttga cgtggctatc gagggtgact caacggctac
     2521 cgcctttaaa ctgacctcac gtcttatcac caacacatta acccagttgg atacctcagg
     2581 ttccacactg aatgtgggcg tggattataa cggcgcggca gtcgaaaaaa ctggcgatac
     2641 cgtgatgatc gataccgcca acggcgtact gggcggcaac cttagcccgc tggcaaacgg
     2701 ttacaatgcc agcaatcgta ccaccgcaca ggatggtttc actttctcca tcatcagcgg
     2761 caccaccaat ggtaccaccg cagtaaccga ttacagcact ctaccggaag gcatctggag
     2821 cggcgacgtt agcgtacagt tcgacgcgac ctggaccagt taatctctct gatgtaccag
     2881 caggggtagc cccccagctt tattcctgga cggaattgtt atgaaaaagc accttctgct
     2941 tctcgctctg ctgttatccg gaatatctcc ggcccaggcg ctggatgtcg gcgatatatc
     3001 atcgtttatg aacagtgaca gcagcacgct gagcaaaacg atcaaaaaca gtaccgacag
     3061 tggtcgcctt atcaatatcc gtctcgaacg gctctcttca ccgcttgacg acgggcaggt
     3121 tatctcaatg gacaagccgg acgagttgct actcactccc gccagcttgc tgctacccgc
     3181 ccaagccagc gaagtgatcc gcttcttcta taagggacca gcagatgaaa aagagcgcta
     3241 ctaccgcatt gtctggtttg atcaggccct cagtgatgcg cagcgcgata atgccaaccg
     3301 cagcgctgtg gccactgctt ccgcccgcat cggcaccatt ctggtcgtcg ccccccgcca
     3361 ggcaaactac cactttcagt acgccaacgg caccctgaca aatacaggaa atgcgacgct
     3421 gcggatcctc gcctacggcc cctgcctgaa agccgccaat ggtaaagagt gtaaagagaa
     3481 ttactacctg atgccgggca agtcgcgtcg ttttacccgc gtggacacgg cggataacaa
     3541 aggacgggtt gcactttggc agggtgataa gttcattccc gtgaaataga tagctgtgca
     3601 gatggataac gacaatgcct ttacgacggt tctccccagg actgaaagcc cagtttgcct
     3661 tcggcatggt ctttttgttc gttcagcccg atgccagcgc tgctgacata agtgcgcagc
     3721 aaataggtgg ggtgattatt ccgcaggcct tcagtcaggc gcttcaggac ggcatgagcg
     3781 tcccgctcta tattcatctc gccggtagcc agggtcgcca ggacgatcag cgaatcggca
     3841 gcgcttttat ctggttggat gatggacagc tacgcatccg gaaaatacag ctggaagaga
     3901 gtgaagataa cgccagtgtc agcgaacaaa ctcgacagca gctgatggct ctggcgaacg
     3961 ccccgttcaa tgaggccctt accatccccc tgactgacaa cgcgcagctg gatctcagct
     4021 tgcgccaact gctgctgcag ctggtggtca agcgcgaagc gctgggcact gtactacgct
     4081 cacgtagcga agacatcggg cagtccagtg ttaacaccct cagcagtaat ctgagctata
     4141 acttcggcat ctataacaac cagttgcgta acggcgggag caacacatcc agctatctgt
     4201 cgctgaataa cgttactgca ctgcgcgaac atcatgtggt gctcgacggc tcgctgtacg
     4261 ggatcggtag cggtcaacag gacagtgaat tatataaagc gatgtatgaa cgcgattttg
     4321 ccggtcaccg atttgccggt ggaacgctcg acacctggaa cttgcagtcc ttagggccga
     4381 tgaccgccat ttcagcaggg aagatttacg gcctttcctg gggaaaccag gccagctcca
     4441 ccatcttcga cagcagccag tcagccacgc cagtgatcgc ctttttaccg gcggcgggtg
     4501 aagtacatct cacccgtgat gggcggttac taagcgttca gaacttcacc atgggcaatc
     4561 atgaagtgga tacccggggt ctaccatacg gtatttacga tgtggaagtt gaggtgatcg
     4621 ttaacggtcg cgtgatcagc aaacgcaccc agcgggtcaa taagctgttt agccgggggc
     4681 gcggcgtcgg tgcaccactg gcgtggcagg tatggggcgg tagctttcat atggatcgct
     4741 ggtcggaaaa cgggaaaaag acgcgaccag ctaaagagag ttggctggca ggtgcctcga
     4801 cctccggctc actgagtacg cttagctggg cggcaacggg atatggatac gataatcagg
     4861 cggtgggtga aacccgtctg acgctgccgc ttgggggagc gatcaacgtt aacctgcaaa
     4921 atatgctggc cagtgacagc tcatggagca gcatcggcag catcagcgcc actctaccgg
     4981 gaggctttag ttcgctgtgg gttaatcagg aaaaaacccg cattggcaat caattgcgac
     5041 gtagcgatgc cgacaaccgt gctatcggcg gcacactcaa cctgaactca ctgtggtcga
     5101 agctgggcac attcagcatc agctacaatg atgaccgccg ttacaacagc cattattaca
     5161 cggcagatta ctatcaaaat gtctacagcg gtacctttgg ttcgcttggc ctgcgggccg
     5221 gtattcagcg ctataacaac ggcgacagca acgccaatac agggaaatat atcgctctcg
     5281 atctctcgct accactgggc aactggttta gcgcagggat gactcatcaa aacggctaca
     5341 ccatggcaaa cctgtcagca cgcaagcagt ttgatgaagg gaccattcgc actgttggtg
     5401 ccaatctgtc acgagccatc tccggcgata ccggtgatga caaaactctc agtggtgggg
     5461 cgtatgcaca gttcgacgct cgctacgcca gcggaacgct gaacgtcaat agcgcggcgg
     5521 acggctacgt caataccaat ttaaccgcca atggcagcgt cggctggcag ggtaaaaaca
     5581 ttgctgccag cgggcggact gatggcaacg ctggggtgat attcaacacc gggctggagg
     5641 acgacggtca gatcagcgcc aaaatcaacg ggcggatttt cccgcttaac ggcaagcgta
     5701 actatctccc gctctctccc tatggaagat atgaggtgga gttacagaac agcaaaaact
     5761 cactcgacag ttacgatatc gtcagcggtc gcaaaagtca tctgactctc tatccaggca
     5821 atgtcgctgt cattgagcca gaggtgaagc agatggttac cgtctccggt cgtatccgtg
     5881 cggaagacgg cacactgctg gctaacgcac ggattaacaa ccatatcggc cgaacccgaa
     5941 ccgatgaaaa cggcgagttt gtcatggacg tggataagaa ataccccact atcgattttc
     6001 gctacagtgg caataaaacc tgcgaagtgg cactggaact caaccaggcg cgcggtgccg
     6061 tctgggtcgg tgatgtggtc tgcagcggcc tctcatcgtg ggcggcggtg acgcagacag
     6121 gagaagagaa tgagagttaa cctactgata gcgatgataa tctttgcgct aatctggcca
     6181 gtaactgcgc tcagagcggc agtgagcaaa acaacctggg cggatgcacc ggcacgcgag
     6241 tttgtgtttg tcgaaaacaa ctcagacgac aactttttcg tcactcctgg cggggcgctg
     6301 gatccgcgcc tgaccggtgc caaccgctgg accggtttaa aatacaatgg ttcaggaacc
     6361 atctatcagc aaagcctcgg ctacattgat aacggttaca acaccggcct ttataccaac
     6421 tggaagtttg atatgtggct ggaaaattca ccagtttcat ctcctttaac tggcttgcgc
     6481 tgcatcaact ggtacgctgg gtgtaatatg accaccagtc ttatcctgcc gcaaaccacc
     6541 gacaccagtg gattttatgg cgcgacggtg accagcggcg gcgcgaagtg gatgcacggc
     6601 atgttgtcag acgcgtttta ccagtatctg caacaaatgc ccgtcggcag cagctttaca
     6661 atgaccatca atgcctgcca gacctctgtg aactatgacg ccagcagcgg cgcacgctgt
     6721 aaggatcagg cctccggcaa ctggtatgtt cgcaacgtca cccatacgaa agcagcaaat
     6781 ctacggttga taaataccca ctcgctggcg gaagtattta tcaacagcga cggagtaccg
     6841 actctgggcg aagggaacgc cgactgccgg acgcaaacca tcggcagccg ttcaggatta
     6901 agttgtaaga tggttaacta taccctgcaa acaaacggac tcagcaacac ctcaatccat
     6961 atattcccgg cgatcgccaa ctcgtcgtta gcctcggccg tcggggcgta cgatatgcag
     7021 ttcagtctga atggcagttc atggaaaccg gtgagcaata ctgcctatta ctacaccttc
     7081 aacgagatga agagcgcaga ctcgatctat gttttcttct cgagcaactt ctttaagcag
     7141 atggtgaacc agggaatcag cgatatcaac accaaagatc tattcaactt tcgctttcag
     7201 aacaccacat caccggagtc tggctggtat gaattttcta cctccaacac gctgattatc
     7261 aaaccccgtg atttcagcat cagtattatc tccgatgaat atactcagac accgtcgcgg
     7321 gagggatatg tcggcagcgg cgagtcggca ctcgatttcg gctatatcgt aaccaccagc
     7381 ggtaaaacag ctgccgacga agtgctgatc aaggtgaccg gacccgcgca ggtgattggc
     7441 gggcgctcct attgtgtctt cagctccgat gacggtaagg cgaaagtacc gttcccggcg
     7501 acgctttcct ttattacccg caacggagct acaaaaacct acgatgccgg gtgcgatgat
     7561 agctggcggg atatgaccga tgcgctgtgg ttgaccacac cgtggactga tatctctggc
     7621 gaagtggggc agatggataa gaccacagtc aaattttcga ttccaatgga taacgccatt
     7681 tctctgcgta cggtagatga taacggctag tttggcgaag tcagcgcttc aggagaaatt
     7741 catgttcagg cgacgtggcg taacattaac taaggccctg ctgacagcgg tctgtatgct
     7801 ggcggcacct ttgacacagg cgatttcggt cggcaatctg actttttcac tgccgtccga
     7861 gactgacttt gtcagcaaac gtgtagtgaa taacaacaaa agcgcgcgga tataccgtat
     7921 tgccatcagt gctatcgata gcccgggcag cagtgaatta cgcacccgac cggtggatgg
     7981 tgaactgctt ttcgcccccc gccagctggc gttgcaggct ggtgagagcg agtattttaa
     8041 attttactat catgggccac aggataaccg cgagcgctac taccgggtct catttcgcga
     8101 ggtccccact cgtaacctga caaaacgcag ccctaccggc ggtgaggtta gcacggagcc
     8161 ggtggtggtg atggatacca ttctggtagt acgaccacgt caggttcagt ttaaatggtc
     8221 cttcgatcag gtgaccggaa cggtaagtaa caccggcaac acgtggttta agctactgat
     8281 taaaccagga tgtgattcga ccgaagagga aggcgatgcc tggtatctac gtccggaaga
     8341 cgtggttcat cagcctgagt tacgtcagcc ggggaatcac tatctggtct ataacgacaa
     8401 attcattaag attagcgatt cctgtccggc taagccccct tcggcggact gatacttatc
     8461 catcggtcga gggaagaatt ccacctcaga gactcaaaaa tcgtttaaat gatgtggaag
     8521 cgatcgtcaa tatcacctgt aacgataggc tttctcatat gcacaccaca gtgttcagga
     8581 gacacggaaa ggtaccaggc ggcctgctaa cgagtggttt cttcactaat taggacgtaa
     8641 aatcggtaac ggctggaaat cattcaatac tcacactatc gaaagtacac cagccaaccg
     8701 cgatacgtcc ctgcatacga cgtaccgcag ttctctttac gatttatagc tgtactggtg
     8761 aattatgagc aatctgaatc catgcatgac gtgtggtgcc tgttgtgcat ttttccgcgt
     8821 ctctttctac tgggccgaag ccgacgatgc tggcggcact attcccgcca ggctcactga
     8881 acaaatatcc ccttttcacc gatgcatgag cggaactaat cagaaaaacc cccgatgtat
     8941 tgcccttgca ggaaccccgg gcaaaaatgc ctgctgcacg atatataaaa atcgatcgtc
     9001 cacatgcaga gaattcgcca tgtctggtga gaacggagtc gtcaatgagg cttgcaatcg
     9061 tgcaagggct aaatacgggc tgacaccgtt ataaatatac aataattatt tgcactgccc
     9121 cgccagctgt aatagcgggg cttcgttttt caggggtaac aaaccccgat acttcttcta
     9181 tttgccagca acaatgcctc tcttctgtag cgttcttcgg atctacatca tcctgaagta
     9241 gcgcgatata ctcactgact atctgaatca gaatataaaa agcaatgttt ttaacctata
     9301 aaaatggcgc tgtatttgcg ccatttttat cattcaatgc attatctgtt tgagcctaaa
     9361 gggatctcag ggtctggctc atgagtaatt ctgtttcgta aatctctgcg aataatttca
     9421 atggaccaga accagactaa atgtccaaaa atttcagaaa cattctcata ccacgggaga
     9481 tcaaacagag gtggtgtcag tcccatgaga gggaatgaaa tcatatgaac aaaaagttgg
     9541 gctaaagcac ctgccagtaa gccctgccag agtttaattt ttggaaatac ttctgcgacc
     9601 acacaataac cgacagcaaa cactatcgaa aagataatgt gcgtaacacc aacccagtta
     9661 aagacatgcc cggcaaaggt ataaacagcc gcattgggat ctgtcagccc cagccagtct
     9721 cgaagaaaaa tatacggagg attgagaaaa ttacgcgagc aatcaatttg cccagcagcc
     9781 ctgattaatg attccgggcc acacgctgca ttaaacatat ccaccgggct acgtggcggc
     9841 aatggaactt cagcccccca cttcacaaat gcggaaacaa cgccagcaat cagcccaatg
     9901 aatgcagcaa gaccataacg tctgcggttc ggtggagttt gttcaaatat attcatatct
     9961 accctgcttg taccattatg ttatacacct ctttaggagt attcataaaa caaggtaaat
    10021 gtaaagaact gtattgtttt gtataacaag atggttgtct aatcaccaaa gaatataagc
    10081 accatcattt ctccctattt ttatattaga agtgacatag atttgcaggg tgatgcagag
    10141 ctgacaatca caggtttcct tatcggtttt tgcatcgtac aactaaagca ataaaccagc
    10201 tcccatcatc atcaacatcc cggcgaacat gaatttatta gccgggctca tagctgcatt
    10261 cgcgttttaa gaataatcct cctgctgtcg ccgactatgc ttaacgttta aaaaagcatc
    10321 agcactctcg caacgcactc ttattttccc ctttagaata ccggaggcct ggtatgagca
    10381 accaaggcga ataccccgaa gataatcggg ttgggaagca tgagccgcac gatttaagtt
    10441 tgacccgtcg cgatctgatt aaagtgagcg ccgcaacggc ggcggccgcc gtggtttatc
    10501 ctcattctac gcttgcggca agcgttccgg cagctacacc cgcgcctgag ataatgcccc
    10561 tgacacttaa ggtgaacggc aaaaccgagc agcttgaggt ggatacccga accacgctac
    10621 tggacgcttt gcgtgaaaat ctgcatttga tcggtaccaa gaaaggttgc gatcacggac
    10681 agtgcggagc ctgtaccgtg ctggtcaatg gtcgcaggct taatgcctgc ctgacgcttg
    10741 cagtcatgca tcagggggcc gagatcacca ccattgaagg cctgggttca ccagataatc
    10801 ttcaccccat gcaggcggcc tttatcaagc atgatggctt ccagtgcggc tactgcacct
    10861 ccgggcaaat ttgctcatca gtagcggtgc taaaagagat tcaggacggc attcccagtc
    10921 acgtcacggt cgatttggtt tcccctccag aaagaactgc cgatgagatc cgtgaacgta
    10981 tgagcggcaa catctgtcgc tgtggtgcat acgctaacat ccttgccgcc attgaagacg
    11041 ctgcggggga gataaaatca tgaaggcgtt tacctatgaa cgagtgaaca cccccgccga
    11101 ggcggcactt agcgctcagc gcgtacccgg cgcaaaattt atcgcgggcg ggaccaatct
    11161 gctggacctg atgaagctgg aaattgaaac gcccacccac cttatcgatg tgaacgggct
    11221 cgggctcgat aagatcgaag tgaccgacgc gggtgggctg cgcatcggcg cactggtacg
    11281 gaacaccgac ctggtggctc acgagcgcgt gcgtcgtgat tacgcggtac tctcccgcgc
    11341 cctgctcgct ggcgcgtctg gtcagttacg caatcaggca accacagcag gtaatctgct
    11401 ccagcgcacg cgctgcccct atttttacga caccaatcag ccctgcaata agcgcctgcc
    11461 cgggagcggc tgcgcggcgc ttgaaggctt tagccgtcag cacgcggtgg taggcgtaag
    11521 cgaagcctgc attgccaccc atccgagcga tatggcggtc gcaatgcggt tgctggatgc
    11581 ggtggtggaa accatcacgc cggagggaaa gactcgcagt atcacactgg ctgattttta
    11641 tcacccaccg gggaaaacgc cgcacattga aaccgccctg cttcccggtg agcttatcgt
    11701 tgcggtgacg ttacctccgc cgctcggcgg aaaacatatc taccgtaagg tgcgcgatcg
    11761 cgcctcctac gcctttgccc tggtatcggt cgcggcgatt attcagcctg acggcagcgg
    11821 gcgcgtcgcg ctgggcggag tagcacataa gccctggcgc attgaggctg cggatgctca
    11881 gctatcccag ggggcgcagg ccgtatatga cgcgctgttc gccagcgccc atcccaccgc
    11941 tgaaaacacc tttaaactcc tgttggcgaa gcgaacgctt gcctccgtac tggctgaagc
    12001 gagggcacaa gcatgaaatt tgataaaccc gcaggggaaa acccgatcga tcagctgaag
    12061 gttgtcggtc gtccccatga ccgcatcgac ggaccgctga aaactaccgg cacggcacgc
    12121 tacgcctacg aatggcatga agaatccccc aacgccgcct atggctatat cgtcggttcc
    12181 gccattgcca aaggacgcct caccgccctt gatacggacg ccgcgcaaaa agcgccgggc
    12241 gtactgcctg tcattaccgc cagtaacgcc ggggcactca gtaaaggcga caaaaacacc
    12301 gccaggctgt taggcggccc cactattgag cactatcatc aggccattgc tctggtagtg
    12361 gccgagacct tcgaacaggc gcgagcggcg gcctcgctgg tgcaggcgca ctatcgccgt
    12421 aataaaggag cttactccct ggcggacgaa aaacaggccg tcagtcagcc gccggtagac
    12481 acgcccgaca agaacgtcgg tgactttgac ggggctttct cctccgctgc ggtgaagatt
    12541 gatgctacct acacgacccc ggaccagagc catatggcga tggagccgca tgcctcgatg
    12601 gccgtctggg atggaaataa gcttactctc tggacctcaa atcagatgat tgactggtgc
    12661 cgcaccgatc tggcaaaaac gctgaaagtg cccgtggaga atgtgcgtat tatctccccg
    12721 tatatcggcg gagggtttgg cggcaagctg ttcctgagaa gcgatgcgct gctggcggcc
    12781 ctcgccgccc gagcggtgaa acgtccggtt aaagtgatgc tcccccgccc ctctattccc
    12841 aataacacca cgcaccgccc cgccaccctt cagcacttgc gtatcggtgc cgaccagagc
    12901 gggaaaatca ccgctatctc acatgaaagc tggtctggaa acctgcccgg cggcacgccg
    12961 gaaacggcgg tacagcaaag cgaattactc tacgccgggg caaaccgtca taccggcctg
    13021 cggctcgcca cgcttgattt gccggaaggg aacgccatgc gtgcgcccgg cgaagccccc
    13081 ggtctgatgg cgctcgaaat cgcgatcgac gaactggcgg aaaaagcggg catcgatccc
    13141 gtcgagtttc gcatcctgaa tgacactcag attgaccccg ccgacccgac gcgccgcttc
    13201 tctcgccgtc agcttatcga gtgcttgcgc accggagcgg ataaatttgg ttggaagcag
    13261 cgcaacgcca cacccggaca ggtgcgcgac ggggagtggc tagtcggcca cggcgtcgcg
    13321 gcgggctttc gcaataatct gctggaaaaa tcgggggctc gggttcacct cgaaccaaac
    13381 ggcaccgtta ccgtggaaac ggacatgacc gacattggca ccggcagcta caccattctg
    13441 gcccagacgg cagcggaaat gcttggcgta ccgctggagc aggttgcggt tcacctcggc
    13501 gattccagtt tcccggtttc tgcgggttct ggtggacaat ggggcgcgaa tacctccacc
    13561 tccggcgttt acgccgcctg tgtgaagctt cgcgaaatga ttgcctcggc agtcgggttt
    13621 gatcctgagc agtcgcagtt tgccgacggc aagattacca acggtacccg aagcgccata
    13681 ctacatgagg ccaccgcagg cggcagactg acagcggaag agagcattga attcggaaca
    13741 ctgagcaagg agtaccagca gtcgaccttt gccgggcatt ttgtggaggt cggcgtgcat
    13801 agcgcgacgg gagaagttcg ggtccggcgt atgctcgctg tgtgtgctgc aggacgcatc
    13861 ctgaatccga aaactgcacg cagccaagtc attggcgcaa tgactatggg catgggcgcg
    13921 gcactgatgg aggagctggc ggtggatgac cgtttgggct acttcgttaa tcacgatatg
    13981 gcggggtatg aggtgccggt ccatgcggat atcccaaaac aggaagtgat tttcctggat
    14041 gataccgacc ccatatcctc cccgatgaag gccaaaggtg tcggtgagct gggcctgtgc
    14101 ggcgtgagcg cggctatcgc caacgcggtg tataacgcca ccggtattcg ggtacgcgat
    14161 tatcccatca ctctggataa gctgctcgat aagctgccgg atgtggttta aggaggaaca
    14221 atgtcatacc cgctttttga taaagacgaa cactggcata agccagagca ggcgtttctc
    14281 accgatgacc accggaccat tctgcgcttc gccgtagagg cgctaatgtc cggtaaagga
    14341 gcggtgctgg tgacgctggt ggagatacgc ggcggcgcgg cgcgcccgct cggggcgcag
    14401 atggtggtgc gcgaagatgg tcgttactgc ggttttgtct ctggcggctg cgtggaggcc
    14461 gctgccgctt ttgaagcgct ggagatgatg ggctcaggct gcgatcgcga aattcgctat
    14521 ggcgaaggtt cgccgtggtt tgacatcgtt ctgccctgcg gcggtgggat cacgctgacg
    14581 ctccataaac tacgctcggc acagcctctg ctcgccgtgc tgaaccgcct ggaacagaga
    14641 aaaccggcgg ggctgcgcta cgatccgcaa gcacaatcgt tggtgtgcct gcccacgcaa
    14701 acccgaacgg gctggaatct caatggcttt gaggtggggt tcaggccatg cgtcaggctg
    14761 atgatttacg gacgttctct tgaggcgcag gcaaccgcga gtcttgcagc agccacaggc
    14821 tatgacagcc atatcttcga tctttttccg gcctcagcca gcgctcagat cgataccgat
    14881 acggcggtca ttttgctgtg ccatgatctc aaccgggagc tgccagtgtt gcaggccgcg
    14941 cgagaagcaa aaccctttta tctcggcgca ttgggcagct atcgaaccca cacgttacgt
    15001 ctgcaaaagc tccacgagct gggatggtcc agggaggaga cagcgcaaat ccgggcaccc
    15061 gtcgggatat tccccaaagc ccggaatgcg catacactgg ccctctccgt gctggcagaa
    15121 gtcgcctctg tacgtctcca tcaggaggag gattcatgcc tgcccccgtc gtcctgatcc
    15181 ttgcggccgg gcgtggaaag cgctttctca cctccggggg aaataccata aattaagaga
    15241 aaaatttgca gtagccgttt tagaaaatca cctaaatcgt ttaaaagagc gccctaccca
    15301 tagtggttgc tcatcgcctt gtgtaacttt ttcagcaacg cgcgccctcg ccggactaaa
    15361 gcgcaccggc cttctggcgt gctgtaacaa aggtcagtac gcccgcgcaa aggagcagca
    15421 cactactcat ggcaaacgta ctctgccagc caaggcggtc aaacacgatg cctcctgccg
    15481 ttgatccaag ggcgattgag agctgaatca ccgcgaccat gagcccgcct ccggcttcgg
    15541 cgttgtcagg caacgtacgc gcaatccatg tccaccatcc ggttggcgcg gcggtggcca
    15601 gcatgcccca gagtcctaac agcagcgaga ccatccagac gtgatgcccg gtaagcatca
    15661 gaccgatggc aatgacggcc atcagtccgg gtatggccat cagcgtgggg tagaattttc
    15721 tgttgagaaa tatcgaaaca accagcgtgc caataaaacc ggccacgccg ataatcagca
    15781 aaatcaacga caagcctaag gaattaaccc gtgtcaccga ctccagaaat gggcgcacat
    15841 aggtgaatag cgcaaactgc cccatgaaaa acaggccaca agctatcatg ccgaccacaa
    15901 ccatgcggcg gctaaataag cggaataccg cgccacatga cgtgtggctt ttattcgcat
    15961 ccatgctggg cagactaatg cactgccaga taaaggcgac aaccgccatg ggcaccaagc
    16021 acaagaaagc gcctcgccat ccgaccgtag cccccagata gcttcccagc ggcgcggcca
    16081 cgaccgtcgc cagcgcatta ccggcgttga aaatcgccag cgcacgcgtg acctgatgct
    16141 gcggcaccag acgaatcgcc gttgccgctg acatcgacca gaatccgcca atggccacac
    16201 caatcatcgc acgaccgacc atatacatca gataactggt agccagcgca ataataagcc
    16261 ccgatacggc catcagaacc gtcatcccca gcagcaggaa cttacgattc atctttccag
    16321 ctagcgttga aagcgtcagg ctggtcagaa ccgccagcgc accggagata gcaatccccc
    16381 gccctgccag cccctccgtc acgcccaggt cacgggcaat cggcgtgagc aggctgacgg
    16441 gcataaattc agaagcaatc agcacaaaca cgcacagggt catggcaaaa ataccgcccc
    16501 agtatgcgtg ctgatgatga ggtgatggtg tttggcttag tgtcgacata gtgaaatttc
    16561 acaaagatca ggataaaagc caccggccat agaccggtgg ctttgcatca attcagggga
    16621 ggttttattt cagtttggtc tggaaaaatt gttcgaactt cgcgaaaggt atttttccgg
    16681 ccacgttatc gtacaggtcc acgtgatttg cccctggtac caccaccaac tctttgtctt
    16741 tactgccaac cgccttgtaa gcatcttcag caaaatagcg tgaatgtgct ttctcaccgg
    16801 tcacaatcag cgtcggaatg gtgatttcac tggcgtagct caacagcggc atattcataa
    16861 atgacagcgg catcgttgcc gtccacgccc cggtagagtt aacggaacgt tcgtggaagc
    16921 cgcgcggcat gcgatagtag tcaaagaact ctttcagtac cggatgagga tttgccggca
    16981 gcgtttctgg cagaatgcgc tcgcccgcac tgaccttgcc gttgctatcg acattaatat
    17041 catgaccacc gtgagcgaac gttccgctct gcgcatcctt ccagcgctgt tcattcaaat
    17101 attgcagaac agcacgacgg tcggcggtag aataacggtc ttcgccatcc cccacaccat
    17161 ggcccatcgc ccggctcatg tcatacatca cgctggtagc aaccgcttta acgcgggtat
    17221 ccatcgccgc gtcgttcagc gccatgccgc cccagccaca aataccgagc agcccgatac
    17281 ggttgcgatc tacctctttt tgcagcccta agaaatccac cgccgcgctg aaatcttcag
    17341 tgttgatatc cggtgaggcg acatttcgcg gatagccgcc gctttccccg gtgtaagaag
    17401 gatcgaacgc cagggtaaca aaccctcgtt ccgccagcgt ctgcgcatac aagccgctgg
    17461 attgctcttt caccgcgcca aatggcccac tgaccgcaat cgccgccagc ttgcgatcgc
    17521 cacggtcttt aggcaggtaa agatcgccca ctaaggtgat gccatatcgg ttcgggaacg
    17581 agactttacg gtgatcgact ttctggctct cggcgaacgt tttatcccat ttatcggtca
    17641 tggacacagg ggcattagga ttggttgaat cagcataact cattgtcgtg actccactta
    17701 atgatgcgaa aagcagcatg accggcatcg cggtttttag cgttcgggtg aaggctttca
    17761 taaaaatccc cataaaaaac aacagagaga tacaggtttc cgacggacaa cgctgcggtg
    17821 ctttactttg atggggtcat tatagtgagc agaattagtg ctgattaggg ggcgattaat
    17881 gctaagatta ataccatatt gttataaatt aatgctgaga ttgccggcat gaaggggaat
    17941 gtttcaatgg caaaaaggga gaactacaac gagctatacc tgtttatgca ggtggtgcgg
    18001 gaaggcagct ttaccgcagc ggctaagcgg ctgggtcttg ctcagtcagg agtcagccgt
    18061 tctgttcgcg agcttgaaga aaggctgggc gtccagctgc tggtgcgcac cacgcgtaaa
    18121 ctgtcgctga cgcaagcagg cgagcagctc taccagaaga cggcatctgg atttgaaatg
    18181 ttagatcttg ggcttgccac gctggcgcac tatcgagaga cgccctccgg cacggtgcgt
    18241 atcaatgcca gccagcacgc gattgataaa tgcctgctgc caaagctggc ggtatttaaa
    18301 cagcgatatc ctgatatcca gcttgaactg ataaatgaga gccggttcgt cgatatcatc
    18361 gaggaaagat tcgatgcagg tgtccgcctg gggcctgaag tcagccaggg catggtcgcg
    18421 gtacgcatca cgcccgatat ggagatggca attgtgggga ctccggaaca ttttcgtcgc
    18481 tatggttttc cgcaaacccc ggcggattta aaggcgcatc cctgtatcgc ctatcagttt
    18541 gccgacggca gcgtatacca gtgggaactc aatcaggatg ataaaaaaat cactcatcaa
    18601 cccgaaggac aatgggcctt ttccgacagc tatatggaag cagaagccgc ccggctgggc
    18661 ctcgggttgg cctatgttcc tgttgaactg gtcgcggatg atttagaaca cggtaagctt
    18721 atcagagtgt tgcagcgtta cagcctgcga atggagggat tgtttctcta ttaccctcat
    18781 cgcaacgtct cccccgctct gcggatggtc attgatacat tgaaaatctg aacgttaaca
    18841 acgaccgact cttcactgtg caatattgtg tcaggtagtc actctgcgtt caattccagg
    18901 ggccgctttg agcgagaaag agcggactta tctgtacaag gggactatcc agcaatggag
    18961 atcatccaaa agaaaaaacc acctggctcc cctcgacaac cttaatccgg gcatttagcc
    19021 tcaatcaaca ttttcatatc tgtcaggtta cttcattatg aaaaccgatc aataccgttg
    19081 tttttgttgc aacagtagaa tacgcgtatt tatccctcca tttctattaa taacggcaat
    19141 aaactgttca tttcactgat atttaaaata tgcatccttt tcaagctgtt cagcggcgta
    19201 agaaaacgta ttaaatatga attcgacgat ttaaccattt ctgtctgatt ggtaatcatc
    19261 tgtgattaat gctggtctat tgattactcc agcattaacc gcttagggat gtattcttcc
    19321 tgttgatata cagccctcga aaaagagaga aaataaaact gtgtccaata ttactctacc
    19381 actccagacc gaagcatcgg tagaattagc agaacgactg gtacaataga ttgggatagc
    19441 gtcatttgtc aggtattcca ttgcctgaca aatgacaatt ttgagtcaat agacgtgctt
    19501 cttgatcatg tcttaaaaat tataccatga gccttatgaa gcatcatttt tactgactgg
    19561 tgttaacttt gctatcatat aaactctgtt cttaaaaaac gcgcccagaa ttttatatga
    19621 tttcccttgt ttttctgcct gtaaagaaat ttctttttca gtgctgttaa tggttgaacc
    19681 gacggcagta atcgttttgg cgacgctact gaatgataat aaagacagtg caaccacaac
    19741 aattaaagaa cagatagtat tcacgctaat ttccctgaca attgagagga tgagttttat
    19801 atataaagac attaaacatg agtcatgttt acttcctgtg aaaaataatc tttctgtaat
    19861 ta
//
LOCUS       DAZKIP010000078        19597 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZKIP010000078 DAZKIP010000000
VERSION     DAZKIP010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.078"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            1..234
                     /gene="yahH"
                     /locus_tag="ACHOUK_003095"
                     /pseudo
     CDS             1..234
                     /gene="yahH"
                     /locus_tag="ACHOUK_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="protein YahH"
     gene            284..1234
                     /gene="yahI"
                     /locus_tag="ACHOUK_003096"
     CDS             284..1234
                     /gene="yahI"
                     /locus_tag="ACHOUK_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFP9898489.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDELQKANPDWRFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTDAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPEKQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            1244..2626
                     /gene="yahJ"
                     /locus_tag="ACHOUK_003097"
     CDS             1244..2626
                     /gene="yahJ"
                     /locus_tag="ACHOUK_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFP9898490.1"
                     /translation="MKENNSRREFLSQSGKMVTAAALFGTSVPLAHAAVSGTTNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQNGKIVALRENKQHPDAT
                     LPHYDAGDKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELAHRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            2925..3335
                     /locus_tag="ACHOUK_003098"
     CDS             2925..3335
                     /locus_tag="ACHOUK_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFP9898491.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTAELARKEGEGDLSL
                     EYWRNEHRRFFQAEGSYSPEMDVIFEEYALIDVV"
     gene            3589..4572
                     /locus_tag="ACHOUK_003099"
     CDS             3589..4572
                     /locus_tag="ACHOUK_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9898492.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGKAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDVSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            4621..6105
                     /locus_tag="ACHOUK_003100"
     CDS             4621..6105
                     /locus_tag="ACHOUK_003100"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFP9898493.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLGFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYLNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQLVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSETKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            6098..7069
                     /locus_tag="ACHOUK_003101"
     CDS             6098..7069
                     /locus_tag="ACHOUK_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9898494.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGCIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            7066..8022
                     /locus_tag="ACHOUK_003102"
     CDS             7066..8022
                     /locus_tag="ACHOUK_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9898495.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVLPILATLGMMTLLKGINILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKAIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQARGMLGLDRVV"
     gene            8109..9158
                     /gene="yahK"
                     /locus_tag="ACHOUK_003103"
     CDS             8109..9158
                     /gene="yahK"
                     /locus_tag="ACHOUK_003103"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFP9898496.1"
                     /translation="MKIKAVGSYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            9401..10216
                     /gene="yahL"
                     /locus_tag="ACHOUK_003104"
     CDS             9401..10216
                     /gene="yahL"
                     /locus_tag="ACHOUK_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFP9898497.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFINSVRTDC
                     KCILLEHHMPLLRLFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNEGISNRVQELGDRYYPVNKHVKIRYTL"
     gene            10667..10876
                     /locus_tag="ACHOUK_003105"
     CDS             10667..10876
                     /locus_tag="ACHOUK_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9898498.1"
                     /translation="MPLLSSLQSGTLPLFGYSGRVRPMKKAHTGESGLKWEAKDSSKL
                     IGNKDHALRGLSSPMVGIRQIRLIT"
     gene            complement(10893..11564)
                     /gene="yahN"
                     /locus_tag="ACHOUK_003106"
     CDS             complement(10893..11564)
                     /gene="yahN"
                     /locus_tag="ACHOUK_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFP9898499.1"
                     /translation="MMQLVHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPINAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            11711..11986
                     /gene="yahO"
                     /locus_tag="ACHOUK_003107"
     CDS             11711..11986
                     /gene="yahO"
                     /locus_tag="ACHOUK_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HFP9898500.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTADIYKKK"
     gene            complement(12084..13670)
                     /gene="prpR"
                     /locus_tag="ACHOUK_003108"
     CDS             complement(12084..13670)
                     /gene="prpR"
                     /locus_tag="ACHOUK_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HFP9898501.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKIPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            13909..14799
                     /gene="prpB"
                     /locus_tag="ACHOUK_003109"
     CDS             13909..14799
                     /gene="prpB"
                     /locus_tag="ACHOUK_003109"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFP9898502.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYHYEEKLDDLFVRSQAK"
     gene            14955..16124
                     /gene="prpC"
                     /locus_tag="ACHOUK_003110"
     CDS             14955..16124
                     /gene="prpC"
                     /locus_tag="ACHOUK_003110"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFP9898503.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVESKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            16158..17609
                     /gene="prpD"
                     /locus_tag="ACHOUK_003111"
     CDS             16158..17609
                     /gene="prpD"
                     /locus_tag="ACHOUK_003111"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HFP9898504.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTGMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVSIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPVFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRARLEQMPVNEYLDLYVI"
     gene            17649..19535
                     /gene="prpE"
                     /locus_tag="ACHOUK_003112"
     CDS             17649..19535
                     /gene="prpE"
                     /locus_tag="ACHOUK_003112"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HFP9898505.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLNQIRQ
                     AMEE"
BASE COUNT         4756 a         4792 c         5313 g         4736 t
ORIGIN      
        1 acgaatggcg caagaatctg taggcctgat aagcgtagcg catcaggcat ttgtcaccat
       61 tgccggatgc ggcgtgaacg ccttatccgg cctacgaatg gcacgagaat tgtaggcctg
      121 ataagcgtag cgcatcaggc atttgtcacc attgccggat gcggcgtgaa cgccttatcc
      181 ggcctacgaa tggcacgaga atttgtaggc ctgataagcg tagcgcatca ggcagtctgg
      241 cgttggtcat aaccccatca ccctctgtag cggacataac aacatgaaag aacttgtggt
      301 cgttgccatt ggtggcaaca gcattatcaa agataacgcc agccagtcga ttgagcatca
      361 ggcggaggcg gtgaaagccg tggccgatac ggtgctggaa atgctggctt ccgattacga
      421 cattgtgctg acccacggta acgggccgca ggtcgggctg gatttacgcc gtgcggagat
      481 tgcccacgag cgcgaagggc tgcccttaac gccgctggcg aactgtgtgg cagatacgca
      541 gggcggcatt ggctacctga tccaacaggc gctgaacaac cggctggcgc gtcacggcga
      601 gaaaaaagcc gtcaccgtgg tgactcaggt ggaagtggat aaaaacgatc caggttttgc
      661 ccatcccacc aagcccattg gtgcattctt cagtgaaagc cagcgtgacg aattacaaaa
      721 ggcaaaccct gactggcgtt ttgttgaaga tgctggacgg ggctatcgcc gcgtggtcgc
      781 ctcgccggaa ccgaaacgta ttgtcgaagc acccgccatt aaggcgctga tccaacaagg
      841 ctttgtggtg attggcgcgg gcggcggcgg aattcctgtg gtgcgtactg acgcgggaga
      901 ttaccaaagc gtggacgcgg ttatcgacaa agatctctct accgcgctgc tggcccgtga
      961 aattcacgcc gacattctgg tgatcaccac cggcgtcgaa aaagtctgta tccactttgg
     1021 taaacccgag aagcaggcgc tggatcgggt ggatattgcc accatgactc gctatatgca
     1081 ggaaggacat ttcccgcccg gcagcatgtt gccaaaaatc atcgccagcc tgacgttcct
     1141 ggaacagggc ggtaaagaag tgattatcac cacgccggaa tgcctgccag cggcgctgcg
     1201 cggtgaaacg ggcacccaca ttattaaaac gtaaggacgt aagatgaaag aaaataatag
     1261 ccgccgtgaa tttctgagcc agagcggtaa gatggtcacc gccgccgcgc tgtttggtac
     1321 ctctgtgccg ctcgcccatg cggcggtctc tggcaccaca aactgcgaag cgaacaacac
     1381 catgaaaatc actgacccgc attactatct cgacaacgtg ctgctggaaa ccggttttga
     1441 ctacgaaaat ggcgtggcgg tgcagacccg cacggcgcgc cagaccgtgg agattcagaa
     1501 cggcaaaatt gtcgcgctgc gcgagaataa gcagcacccg gatgccacgc tgccgcacta
     1561 tgacgctggc gataagctga tgctgcccac cacccgcgac atgcatattc atctcgacaa
     1621 aacgttttac ggcgggccgt ggcgctcgct caatcgcccg gcaggcacca ccatccagga
     1681 catgatcaaa ctcgagcaga aaatgctgcc ggagctgcaa ccgtacactc aggagcgggc
     1741 agaaaaactg attgatttat tgcagtcgaa aggcaccacc attgcccgca gccactgcaa
     1801 tatcgaaccg gtttccggcc tgaaaaatct gcaaaatttg caggcggtgc tggcgcgacg
     1861 tcaggcgggc tttgagtgtg aaatcgtcgc cttcccgcag cacggtttgc tgctgtcgaa
     1921 atcggaaccc ttaatgcgtg aagcaatgca ggcgggtgcg cattacgtcg gcgggctgga
     1981 cccgaccagt gtcgatggcg cgatggaaaa atccctcgac accatgttcc agattgcgct
     2041 ggactacgac aaaggcgtcg atattcacct gcacgaaacc actccgtcgg gcgtggcagc
     2101 catcaattat atggttgaaa cggtagagaa aacgcctcaa ctgaaaggta agctgaccat
     2161 cagccacgcc tttgcgctgg ctacgctcaa cgagcaacag gtagatgaac tggcgcatcg
     2221 catggcggcg cagcaaattt ctatcgcctc gacggtgccg attggcacgc tgcatatgcc
     2281 gctcaaacag ttgcacgaca aaggcgtaaa agtgatgact ggcactgaca gcgttatcga
     2341 ccactggtcg ccttatggtc tgggcgacat gctggaaaaa gccaatctct acgcgcagct
     2401 ctatattcgt cctaacgaac agaacctctc ccgttcgctg tttctagcca ctggcgatgt
     2461 attgccgctg aatgaaaaag gcgagcgtgt atggccaaaa gcgcaggatg acgccagctt
     2521 tgtgctggtg gacgcctcct gttccgccga ggcggtggcg cgtatctcgc cgagaaccgc
     2581 aacgttccat aaagggcaac tggtgtgggg gagtgtggca ggttgatgcg catttgaaac
     2641 gcctgcaacg gtgaatagta agagatttaa gccccaggga gtggggcggg aatggtttgc
     2701 taatgaatga tcctgaactt tataatctcc cggcataatt tccaggggat tatttgagag
     2761 aaatacacaa aagtgagttt taacaaaggt atttatcagg ccataaatgt gacctgcttc
     2821 ggataaaaaa gattcagtat cgaaaaacca atgatactct tgctgatggt taccaacagt
     2881 aattaagatg tgatttgttt ttccgtaatc gcaaaaatat tgccatgacc ataaaagaaa
     2941 aattctcaca aaaatatccc catgcttcct tttgtacttt tggcgattca gccgcgttgg
     3001 ccgatcacct cgcaacgttg attgcgactg gtgttaaaac agcttcctgt ggttcgctgg
     3061 cgggatgtat tgaagacaac gcatttccga tgattggtga gtataagatt gtagaaaata
     3121 gccgtggtga gcctgtatgt gttatcagag tgattggcct gcatttactg cgcttttctg
     3181 atgtcaccgc ggagcttgcc cgtaaggaag gtgaaggtga cctcagtctt gaatattggc
     3241 gtaatgaaca ccgtcgtttt ttccaggcag aaggaagcta ttcaccagaa atggacgtta
     3301 tctttgaaga atatgccctt attgacgttg tgtagataac tatttatcta catgaaataa
     3361 gctcacaaac ataaaatatc gctaattttg tttattttgc tgttttttac gtgatgaatg
     3421 tcacacttat tattgctgcc cgttttgtcc atatttataa ccatattttt aatagctgcc
     3481 tgaaatagag ctgtttattc tgcataaaaa tattcgaaaa ttcttattct ctccagcgcg
     3541 ctaatttggg cgaacacttc ctgactaccc tgcaatgagg ctgaaatgat gaataaacgt
     3601 tttgttatca atatggtgtc ttcgctgtta ttaggcgctg cgctaatttc tgcgccgctc
     3661 caggcagcgg aaaaagtcgt ggtgaatatt tctaaagtcg atggtatgcc gtggtttaat
     3721 cgcatgggcg aaggtgtggt tgaggcgggt aaagcctttg gtgttaatgc ctcacaggtg
     3781 gggccttcaa gtactgatgc tccgcaacaa gttaaaatta ttgaggattt aatcgcccgt
     3841 aaggttaatg caattactat cgtgccaaac gatgccaacg tgctggaacc ggtattcaaa
     3901 aaagcgcgag atgccggaat tgtggttctg accaacgaat cccccggtca gccaagtgct
     3961 aactgggata tcgaaatcat tgataacgaa aaatttgccg ctgaatatgt tgagcatatg
     4021 gcgaaacgca tgggtggcaa gggcggctat gtgatttacg tgggtagttt gaccgtgccg
     4081 cagcataact tatgggctga cttgctggtg aaataccaga aagagcatta cccggatatg
     4141 catgaagtta cccgccgcat gccggttgcc gagagtgtgg atgtctcacg tcgtactaca
     4201 ctggacctga tgaaaaccta tcctgacttg aaagcggttg tctcctttgg ttcaaacggt
     4261 ccgattggcg cgggacgtgc ggtgaaagag aaacgggcga aaaataaagt cgcagtctat
     4321 ggcatgatga tcccttcaca ggcggcatca ctgattaaaa gtggtgatat caccgaaggg
     4381 attacttacg atccggcctc tgcaggttac gcactcgcag ccgtcgccag cacgttgctg
     4441 aagggtgaag agatcaagcc tggattagaa atgcagaatc tgggtaaggc cgacgtagat
     4501 atggataagc gaattatccg tttccataag gtattactgg tcaacaaaga caatatcgac
     4561 tcactttatt aatccatttc ttcggggcga aagccccgat tcttattgcc aggggatgtt
     4621 atggaaacct tcctttccct tcgtcatatc aacaaaacgt ttcatgccac gcgagcgctg
     4681 cgcgatgttt ctctcgactt tatgtcgggt gaagtgcact gccttgccgg gcaaaacggc
     4741 tgtggtaaat cgacgttgat taaaattatg tccggtgttt atcgcccgga tgagggagcg
     4801 gaaattacgc ttggtgggaa aaactggtca aagctgacac ccgccgcttc ggtggcgcag
     4861 gggattcagg tgatttatca ggacctctct ttatttccta acctgagtgt ctgggagaat
     4921 atcgccgtga atcactatca ccacggtctg tttgttaacc gccgtcgtct gcgtgaggtg
     4981 gcgcaggcgg caatgactag tattaacgtc acattgccac tggatacgct cgtttctgag
     5041 ctttcaattg cccgctgtca gctggtggca atttgccgtg cactggcgca ggacgcacgg
     5101 ttgatcgtga tggatgaacc aaccgcttcg ctgacccatc aggaagtgca agggttatta
     5161 caggtggtgc atcaattgcg tgaacgcggg atctgcgtgg tctttgtcag tcatcgtctg
     5221 gaagaagtga tggaagtttc cgaccgtatt tcagtgctga aagatggtga gctggtcggg
     5281 acttttccgg ctgcagagat gaccacaaaa caactcggtt tcctgatgac cggccaggag
     5341 tttgaatatc aggtgcgaga gttgtggcag ggaaaatcca gcaccccggt gctggaggtg
     5401 cgtaatttaa gtcgtcatgg ggaatatcta aatattaact tgcgggtgga agcgggcgaa
     5461 gtggtatcga ttgttggcct gcttggtgcg gggcgcacgg aattatgtct gagtctgttt
     5521 ggtatgaccc gaccagatgc tggcgagatc ctcatcaacg gccagctggt tacactgcat
     5581 agtaaccagg acgccatccg ccacggtatt ggttatgttt cggaagatcg catgtcgcgc
     5641 ggtttggtga tggcgcaatc tattgaagac aacatcatta gcactgtttt tcacaaggta
     5701 aaagatcgct tcggctttct gagtgaaact aaagtgcgtg acctggtgga caggctaatc
     5761 aaggcattga ccatcaaagt ctctgatcct catcttccgg tgaatacgct ttctggtggt
     5821 aacgcgcagc gagtatcgat cgccaaatgg ctggcgattg ggccacgact gttgattctc
     5881 gatagcccga cagtcggtgt tgatatcgcc aacaaagcag gaatatacgg cattatcagc
     5941 gatctggcgg ctcatggcat tgcggtattg atgatctgcg atgaaattga agaagcgtgg
     6001 tatcaaagcc accggatttt ggtaatgcaa aaaggtcaga tcacccatag cttcctgcct
     6061 gacagcagtt ctcaggccag gattgcggag gtggtaaatg gctgaactga aaaaacgcca
     6121 tgaattctgg ctggcattac tgattgtggt gctgtttgtt ggccttgcct ggcgcagcga
     6181 cgagtttctg acattcggta atttgtacga tctcgccaat aactatgcca tgttgactat
     6241 tctcgcctgt ggtttgtttg tggtgttgat ttccggtgga attgatattt cgtttccagc
     6301 aatgaccatc attgcgcaat acggcatggt gctgttgctg caaaaaattg gtggcaactt
     6361 cgctgtcgcg tttgcactgg caggctgcat cggcatttta cttggcttaa ttaacgcctt
     6421 actggttaat cgcctacggg tgccttctat catcatcact atctcgacgc tgaatatttt
     6481 ctatggcctg ctgttatggt tgagtaaagg tgtgtggctg tacgactttc cgccgtggtt
     6541 tgagcagggg gttatgttgt tcaagtacac cgatgctgat ggctatgact atggccttgg
     6601 tctgccgctg atcgccatga ttacggtggt gctgctgaca gcgtttatca tgaatttcac
     6661 cagtgtaggg cgcaaaattt atgcccttgg cgggaaccgc gaatcagcca gtcgcatcgg
     6721 ctttagcgtg ctgaaactgc aacttttcgt ctacggctat atgggattga tgtctggcgc
     6781 tgcgggtgta gtgcagtcgt ggacggtgat gactgtcgcc cccgattctc ttctgggtta
     6841 tgagctgaca gtactggctg cggtggtgct tggcggcact agtttgctcg gcgggcgcgg
     6901 cacgttaact ggtactttgc tcggcgtggt gttgttggca gtgatgcaaa acgggctaaa
     6961 tttattggga gtctcgtctt actggcaaac attgatcacc ggcatcatca tcgttgccag
     7021 cattagcgcc acggcgtgga gtcagcatca gaaccggagc ctgctatgaa aaaatcgtgg
     7081 cgtaataacg ttgagtttta cttgatcggc ctgctggtgc taactgttgc cgcgttcagc
     7141 attactatgc cggagatttt ctggtcgata agtaatttcc agtccgtcgc ttcgcaaatg
     7201 cccgtgctgg gcattctggc gttggctatg gcggtgacca tgctttgcgg tggcattaac
     7261 ttgtcaatta tagccactgc taacgcctgt tcactggtga tggcgtgggt ggcgactcag
     7321 tatccaccgg gtattgctac ggttgtcgct acgttactgg cgggcgctgg cgcggcggtg
     7381 ataatcggtt tatgtaacgg cgtattgatt gctggtattc gagtgttgcc aattcttgca
     7441 acgcttggga tgatgacgtt gctcaaaggg ataaatattc tggtgacagg tggtagcgcc
     7501 attgccaatt atccctcatg ggtgctgtgg ttgaatcatg cgcaatggtt cggtattccg
     7561 ctaccgatgt ggttgtttgc cgcagtggca ctggggctgt ggatattgct ggagaaaacg
     7621 ccgctcggta aagccattta cctgattggg tctaacgaac gagcaacact ttactccggc
     7681 attaacactc gtcgggtgct gatttgggtt tatgtcattt ccgccttgct ttgtgcggta
     7741 gcggcatttt tgatgatgtc gaaactcaac tcggctaagg cctcttatgg cgaatcgtac
     7801 ttgctggtgt caatcctcgc ggcagtactc ggcggggtta atccggacgg cggcagcggg
     7861 cggataattg gtatggtgct ggcgctgttt cttctgcaaa ttattgaaag cggcttcaat
     7921 attctcggta taagccccta cctgacgatg gccttatggg gaacgttgct gctctgtttc
     7981 attcaggctc gcggcatgtt ggggctggat cgggtggttt aaatattggc tatattcaat
     8041 ggacgcgttt tgccgcgatg acatatcagg cgtcgccaaa tacacatagc taatcaggag
     8101 taaacacaat gaagatcaaa gctgttggtt catattccgc taaacaacca cttgaaccga
     8161 tggatatcac ccggcgtgaa ccgggaccga atgatgtcaa aatcgaaatc gcttactgtg
     8221 gcgtctgcca ttccgatctc caccaggtcc gttccgagtg ggcggggacg gtttacccct
     8281 gcgtgccggg tcatgaaatt gtggggcgtg tggtagccgt tggtgatcag gtagaaaaac
     8341 atgcgccggg cgatttggtc ggtgtcggct gcattgtcga cagttgtaaa cattgcgaag
     8401 agtgtgaaga cgggctggaa aactactgtg atcacatgac cggcacctat aactcgccga
     8461 cgccggacga accgggccat actctgggcg gctactcaca acagatcgtc gttcatgagc
     8521 gatatgttct gcgtattcgt cacccgcaag agcagctggc ggcggtggct cctttgttgt
     8581 gtgcagggat caccacgtat tcgccgctac gtcactggca ggccgggccg ggtaaaaaag
     8641 tgggcgtggt cggcatcggc ggtctgggac atatggggat taagctggcc cacgcgatgg
     8701 gggcacatgt ggtggcattt accacttctg aggcaaaacg cgaagcggca aaagccctgg
     8761 gggccgatga agttgttaac tcacgcaatg ccgatgagat ggcggctcat ctgaagagtt
     8821 tcgatttcat tttgaataca gtagctgcgc cacataatct cgacgatttt accaccttgc
     8881 tgaagcgtga tggcaccatg acgctggttg gtgcgcctgc gacaccgcat aaatcgccgg
     8941 aagttttcaa cctgatcatg aaacgccgtg cgatagccgg ttctatgatt ggcggcattc
     9001 cagaaactca ggagatgctc gatttttgcg ccgaacatgg catcgtggct gatatagaga
     9061 tgattcgggc cgatcaaatt aatgaagcct atgagcgaat gctgcgtggt gatgtgaaat
     9121 atcgttttgt tatcgataat cgcacactaa cagactgaaa aaattaataa atacgctgtg
     9181 gtttaacata ttaacttcgc tctccactta actttttagt taaggagagc gaaataatat
     9241 caaagtagca gtaaaaccta taacgtaaat tcaaattgtt aaattaactc cctccagtac
     9301 ctaatacttc acacgttagt tatgagcgat ttctgatagt gcctggttta atcagagctt
     9361 tattatctgc gacgtttatt tttatttaag agagagcgcg atgatatcat taaaagctcc
     9421 gcacaataat ttgatgccat atacgcaaca aagcatactt aatacggtta aaaacaatca
     9481 gttaccagag gatatcaaaa gctccctggt ttcctgtgtc gatatattca aggttttgat
     9541 taaacaatat tatgattatc cttatgattg tcgtgatgat ttagtcgatg acgacaaact
     9601 cattcatctc atggctgccg tacgagactg tgagtggtca gacgataacg cactcaccat
     9661 aaatgtgcag tttaatgatt tccctggatt ttatgactgg atggattacc ctgatcaccc
     9721 ggttaagttt gtttttcgca tacttgaaaa tcaaaaaggg acagtttggg tatatgacca
     9781 ggatgatgcg tttcttgata ttaaagcaaa tgtgcaggct ggacgcttta ccgggcttaa
     9841 gaaattagtg cagtttatca attcagtgcg aacagattgc aaatgtattc ttcttgaaca
     9901 ccatatgcct ttgctgagac ttttccctaa agggaaagag tgtatgcatg tggaaaaatg
     9961 gcttcgcgaa atgtcgtcta ttccggagac agacgcgccc attaagcagg cgcttgcgca
    10021 tggattatta ttacacttaa aaaatatcta ccctgttttt cctgaatcac tggtgatgtt
    10081 attgctctct gttctggacg taaaaacata cagagatgat gcacgattaa atgaagggat
    10141 atctaataga gtccaggaat taggtgacag atattatcct gtaaataagc acgttaaaat
    10201 ccgctacact ctttagttca gaaagggttc cacctgcaag ttgttattct acctgagagt
    10261 gaatcgcaat gcaggtaatc atttcatctg tatatctgta tgccaactgg cattccatct
    10321 cttttttcgc cgatattttg ttgtgatcgg cgacacttcg cttaaaaaag caccagtagt
    10381 ggtttcgcag ccatgcggtg tataaaaaaa atgatctcat gcagatgttt tgtgaatgtg
    10441 tgggttgaca ttcatatgaa aaaaatcata attccatcat gtttgtgtgg ggtcttttct
    10501 gtatcttacg catcgcactc aagcctgaca gaaaatatgc tgtaaggctc atatcaaaaa
    10561 ccgccattag ctcatcagga agagcagacg acaaccagga ttgttgtatg gtacggggtt
    10621 cgaggccccg atggcggtcc aacttttcaa aactcttctg aattagatcc ccttattatc
    10681 atccctgcaa agtggaacat tgccattgtt tggctattca ggacgggtca gaccaatgaa
    10741 aaaagcccac acaggggaga gtgggctgaa atgggaagct aaagactcaa gtaaacttat
    10801 cggaaataag gaccacgcat tacgggggct atcatcgccg atggtcggga ttcggcaaat
    10861 caggttgatt acgtagagag taaattatct gctcaccgct gcgtcacccc ttcgtaaatc
    10921 aggcgtagcg cgaatacacc aataattgca ccaataaccc gactggcaac gcgttgcata
    10981 cgcccataag cacgacgcac agcgggcaaa gaaaacgcct gactaagaaa aactcgccag
    11041 ataattgatg cgagcacaat ccccgcccag gccattaaac gtgcccatgt tggtgtttcg
    11101 gcatttaatg ttactgagaa aatactaata aaaaataaaa cggtttgtgg gttagagaga
    11161 tcggtaatta atccacggcg aaaaaagaca taccaggggg cgttaatcgg ttgttgtagt
    11221 gtgctcattt gcggtgttga ctggcggcgc atgctgcacc acgcaaacca taagagataa
    11281 gcgccgccga cgattctgat aagcgaaaaa atctcctcac actgcgtaat tagcgttgca
    11341 agaccaaaca aacccaaccc ggaataaaat gcatcgccca gcgccacgcc cagtccggtc
    11401 agcactcctg cgcgtcgacc ggaagccagg ctggtttgta ctaccacaaa aagattggct
    11461 cccggattaa aaaaagtaat cacgaacagt cctacggtca ggtaaacggc atgcaaagga
    11521 tccatagtga tttcatccat aaataagtga actaactgca tcatatttct accaaaaata
    11581 atcgggtgcg agagagatca caaagtgtct tatttccggt tactggcgtt tatgccctga
    11641 ctgaactaat tattaatcaa cccaataatg tcggtgggtg atagtgtgat aacaactctg
    11701 gagccgtaat atgaaaataa tctctaaaat gttagtcggt gcgttagcgt ttgccgttac
    11761 caatgtctat gccgctgaat tgatgaccaa agcggaattt gaaaaagttg aatcgcagta
    11821 tgaaaaaata ggtgatattt caaccagtaa tgaaatgtcg actgcagatg caaaagaaga
    11881 tttaatcaaa aaagccgatg aaaaaggggc tgatgtgttg gtactgacct ccggtcaaac
    11941 tgacaataag atccacggca cggcggatat ttataagaag aagtaattct gaatcctatg
    12001 taaacatccc cgatgcgtaa gtttatcggt gatcatctat tgaaatttat gccggataaa
    12061 gcgttcgcgc tgcattcggc agttcagctt ttcagccgcc gccagaacgt cgtccggctg
    12121 atgcctaaat aattcgccgc tgctgtttta tcgccattaa atttctccag tgcctgttgt
    12181 ggtgtcagca agcgtggagc gggaattttc gccgactcgc gcgccagttc cggcagtagc
    12241 agctgcaaaa actgcggcgt taaatccggc gtcggttcca cacttaaaaa tagcgccagt
    12301 cgctccatca tattgcgcag ttcacgaata ttacctggcc agtcgtagtg caccagcacg
    12361 gtttcgcttg cctgtaatcc ctggcgtaat gcggcagaaa atggggcgga gagcgccgcc
    12421 agagacactt tcaaaaagct ttccgccagc ggaagaatat ctgccacccg ctcgcgcagt
    12481 ggtggcaatt gcagacgcaa aatactcagc cgataaaaca gatcacggcg aaactgccct
    12541 tgccgcatat cttcttccag attgcagtga gtggcgctaa tgacccgtac atctaccgga
    12601 acaggctgat gcccgccgac gcgggtgacc tctttttctt ccagcacccg taacaggcga
    12661 gtctgcaaag gtagcggcat ttcgccaatc tcatccagaa acagcgtgcc accgtgggca
    12721 atttcgaaca gcccggcgcg acctccgcgt cgcgagccgg taaacgcccc ttcctcatag
    12781 ccaaacagct ccgcttccag cagcgattcg gcaatcgccc cgcagttgac ggcaacaaac
    12841 ggatgcgact ttttgccctg tcgcgcatcg tggcgggcaa aatattcccg atgaattgcc
    12901 tgggccgcca gctctttgcc cgtccccgtt tccccctcaa tcaacaccgc cgcactggag
    12961 cgggcataca gcaaaatagt ctgccgcacc tgctccatct gtggtgattg accgagcata
    13021 tcgcccagca cgtaacgagt acgcagggcg ttgcgggtgg catcgtgagt gttatggcgt
    13081 aacgacatgc gcgtcatatc cagcgcatcg ctgaacgcct ggcgcacggt ggcggcggaa
    13141 tagataaaaa ttccggtcat tccggcttct tctgccagat cggtaatcag ccccgcgccg
    13201 accaccgctt cggtgccgtt agcttttagc tcgttaatct gcccgcgtgc gtcttcttcg
    13261 gtaatgtagc tacgttgatc gaggcgcaaa ttaaaggttt tttgaaacgc caccagtgcc
    13321 ggaatagttt cctgataagt gacaacgccg atagaagagg tgagttttcc ggcttttgcc
    13381 agtgcctgta acacatcgta gccgctcggt ttaatcaaaa taactggcac tgacaggcgg
    13441 cttttcaggt acgcgccgtt agagccagcg gcgatgatgg cgtcacagcg ttcgtttgcc
    13501 agtttcttgc ggatgtaggt cactgctttt tcaaagccaa gctgaatagg ggtaatgttc
    13561 gccaggtgat caaactcgag gctgatatcg cgaaacagct cgaataagcg cgttacagat
    13621 accgtccaga taaccggttt gtcgtcattc agccgtggtg gatgtgccat agcgcaccgc
    13681 aaagttaaga taccgaatat tgggtttagt cttgtttcat aattgttgca atgaaacgcg
    13741 gtgaaacatt gcctgaaacg ttaactgaaa cgcatatttg cggattagtt catgacttta
    13801 tctctaacaa attgaaatta aagatttaat tttattaagg caattgtggc acaccccttg
    13861 ctttgtcgtt atcaacgaaa ataacaagtt gataacaaag gatgggctat gtctctacac
    13921 tctccaggta aagcgtttcg cgctgcactg actaaagaaa atccattgca gattgttggc
    13981 accattaacg ccaaccatgc gctgttggcg cagcgtgccg gatatcaggc aatttatctc
    14041 tccggcggtg gcgtggcggc agggtcgctg gggctgcccg atctcggtat ttctactctt
    14101 gatgacgtgt tgacagatat tcgccgtatc accgacgttt gttcgctgcc gctgctggtg
    14161 gatgcggata tcggttttgg ttcttctgct tttaacgttg cgcgtacggt gaaatcgatg
    14221 attaaagccg gtgcggcggg attgcatatt gaagatcagg ttggtgcaaa acgctgtggt
    14281 catcgcccga acaaagcgat cgtctcgaaa gaggagatgg tggatcggat ccgcgcggcg
    14341 gtggatgcga aaaccgatcc tgattttgtg atcatggcgc gcaccgatgc gctggcggta
    14401 gaggggctgg atgcggcgat cgagcgtgcg caggcctatg ttgaagcggg tgccgagatg
    14461 ttgttcccgg aggcgattac cgaactcgcc atgtaccgcc agtttgccga tgcggtgcag
    14521 gtgccaatcc tcgccaacat taccgaattt ggcgccacgc cgctgtttac caccgacgaa
    14581 ttacgcagcg ctcatgtcgc gatggcgctg tacccgcttt cagcgtttcg cgccatgaac
    14641 cgcgccgccg aacatgtcta caacgtcctg cgccaggaag gcacgcagaa aagcgtcatc
    14701 gacaccatgc agacccgcaa cgagctgtac gaaagcatca actactacca ctacgaagag
    14761 aagctcgacg acctgtttgt ccgtagccag gcgaaataaa aaacgcccgt tggttgtatt
    14821 cgacaaccga tgcctgatgc gccgctgacg cgacttatca ggcctacggg gtgaactgta
    14881 ggtcggataa gacgcatggc gtcgcatccg acaacaatct cgaccctaca aatgataaca
    14941 atgacgagga caatatgagc gacacaacga tcctgcaaaa cagtacccat gtcattaaac
    15001 cgaaaaaatc tgtggcgctt tctggcgttc cggcgggcaa tacggcgctc tgcaccgtgg
    15061 gtaaaagtgg caatgacttg cattaccgtg gctacgatat tcttgatctg gcggaacatt
    15121 gcgaatttga agaagtggcg catctgctga tccacggcaa actgccgacc cgtgacgaac
    15181 tcgccgccta caaaacgaaa ctgaaagccc tgcgcggttt accggctaac gtgcgtaccg
    15241 tgctggaagc cttaccggcg gcgtcacacc cgatggatgt tatgcgcacc ggcgtttccg
    15301 cgctcggctg cacgctgcca gaaaaagagg ggcataccgt ctctggcgcg cgggatattg
    15361 ccgacaaact gctggcgtca cttagctcga ttcttctcta ctggtatcac tacagccaca
    15421 acggcgaacg catccagccg gaaaccgatg acgactccat cggtggtcac ttcctgcatc
    15481 tgctgcacgg cgaaaagccg tcgcaaagct gggaaaaggc gatgcatatt tcgctggtgc
    15541 tgtacgccga acacgagttt aacgcctcca cctttaccag ccgggtgatt gcgggcactg
    15601 gctctgatat gtattccgcg attattggcg cgattggtgc actgcgcggg ccgaagcacg
    15661 gcggggcgaa tgaagtgtcg ctggagatcc agcaacgcta cgaaacgccg gacgaagccg
    15721 aagccgatat ccgcaagcgg gtggaaagca aagaagtggt cattggtttt ggtcatccgg
    15781 tttataccat cgccgacccg cgccaccagg tgattaaacg tgtggcgaag cagctctcgc
    15841 aggaaggcgg ctcgctgaag atgtacaaca tcgccgatcg cctggaaacg gtgatgtggg
    15901 agagcaaaaa gatgttcccc aatctcgact ggttctccgc tgtttcctac aacatgatgg
    15961 gcgttcccac cgagatgttc acaccactgt ttgttatcgc ccgcgtcacc ggctgggcgg
    16021 cgcacattat cgaacaacgt caggacaaca aaattatccg tccttccgcc aattatgttg
    16081 gaccggaaga ccgccagttt gtcgcgctgg ataagcgcca gtaaacctct acgaataaca
    16141 ataaggaaac gtacccaatg tcagctcaaa tcaacaacat ccgcccggaa tttgatcgtg
    16201 aaatcgttga tatcgtcgat tacgtcatga actacgaaat cagctccaaa gtagcctacg
    16261 acaccgcaca ttactgtctg ctcgacacgc tcggctgcgg tcttgaagct ctcgaatacc
    16321 cagcctgtaa aaaactgctg gggccaattg tccccggcac cgtcgtacct aacggcgtgc
    16381 gcgtccccgg aactcagttc cagctcgacc ccgtccaggc ggcatttaac atcggcgcga
    16441 tgatccgctg gctcgatttc aacgatacct ggcttgcggc agagtggggc catccttccg
    16501 acaatctcgg cggcattctg gcaacggcgg actggctttc acgcaacgcg gtcgccagcg
    16561 gcaaagcgcc gttgaccatg aaacaggtgc tgaccggaat gattaaagcc catgaaattc
    16621 agggctgcat cgcgctggaa aactccttta accgcgtcgg cctcgaccac gttctgttag
    16681 tgaaagtggc ttccaccgcc gtggtcgccg aaatgcttgg cctgacccgc gaggaaattc
    16741 tcaacgctgt ttcgctggcg tgggtggacg gtcagtcgct gcgcacctat cgccatgcgc
    16801 cgaacaccgg cacgcgtaaa tcctgggcgg cgggcgatgc cacttcccgc gcggtacgtc
    16861 tggcactgat ggcgaaaacg ggcgaaatgg gttacccgtc agccctgact gcgcctgtgt
    16921 ggggcttcta cgacgtctcc tttaaaggtg aatcgttccg cttccagcgt ccgtacggtt
    16981 cttacgtgat ggaaaatgtg ctgttcaaaa tctccttccc ggcggagttc cactcccaga
    17041 cggcagttga agcagcgatg acgctctatg aacagatgca ggcagcaggc aaaacggcgg
    17101 cagatatcga aaaagtgtcc atccgcaccc acgaagcctg tattcgcatc atcgacaaaa
    17161 aggggccgct caataacccg gcagaccgcg accactgcat tcagtacatg gtggcgatcc
    17221 cactgctatt cgggcgctta acggcggcag attacgagga caacgttgcg caagataaac
    17281 gcattgacgc cctgcgcgag aagatcaatt gctttgaaga tccggtattt accgctgact
    17341 accacgaccc ggaaaaacgc gccatcgcca atgccattac ccttgagttc accgacggca
    17401 cacgatttga agaagtggtg gtggagtacc ccattggtca tgctcgccgc cgtcaggatg
    17461 gtattccgaa actggtcgat aaattcaaaa tcaatctcgc gcgccagttc ccgactcgcc
    17521 aacagcagcg cattctggag gtttctctcg acagagctcg cctggaacag atgccggtca
    17581 atgagtatct cgacctgtac gtcatttaag taaacggcgg taaggcgtaa gttcaacagg
    17641 agagcattat gtcttttagc gaattttatc agcgttcgat taacgaaccg gagcagttct
    17701 gggccgagca ggcccggcgt attgactggc agacgccctt tacgcaaacg ctcgatcaca
    17761 gcaatccgcc gtttgcccgt tggttttgtg aaggccgaac caacttgtgc cacaacgcta
    17821 tcgaccgctg gctggagaaa caaccagagg cgctggcgct gattgccgtc tcttcggaaa
    17881 cagaagaaga gcgcaccttt acctttcgcc agcttcatga cgaagtaaac gcggtggcgt
    17941 caatgctgcg ctcgctgggc gtgcagcgtg gcgatcgggt gctggtgtat atgccgatga
    18001 ttgccgaagc gcatattact ctgctggcct gcgcgcgcat tggcgctatt cactcggtgg
    18061 tgtttggggg atttgcctcg cacagcgtgg cggcgcgaat tgatgatgct aaaccggtgc
    18121 tgattgtctc ggctgatgcc ggagcgcgcg gtggcaaaat cattccctat aaaaaattgc
    18181 tcgacgatgc gataagtcag gcgcagcacc agccacgcca tgttttgctg gtggatcgcg
    18241 ggctggcgaa aatggcacgc gtcagcgggc gggatgtcga tttcgcgtcg ttgcgccatc
    18301 aacacatcgg cgcgcgggta ccggtggcgt ggctggaatc caacgaaacc tcctgcattc
    18361 tctacacctc cggcacgacc ggcaaaccta aaggcgtgca gcgtgacgtt ggcggatatg
    18421 cggtggcgct ggcgacctcg atggacacca tttttggcgg caaagcgggc ggtgtgttct
    18481 tttgtgcttc ggatatcggc tgggtggtgg ggcattcgta tatcgtctac gcgccgctgc
    18541 tggcggggat ggcgactatc gtttacgaag gattaccgac ctggccggac tgcggcgtgt
    18601 ggtggaaaat cgtcgagaaa tatcaggtta gccggatgtt ctcagcgccg accgccattc
    18661 gcgtgctgaa aaaattccct accgctgaaa ttcgcaaaca cgatctctcg tcgctggaag
    18721 tgctctatct ggctggagaa ccgctggacg agccgaccgc cagttgggtg agcaatacgc
    18781 tggatgtgcc ggtcatcgac aactactggc agaccgaatc cggctggccg attatggcga
    18841 ttgctcgcgg tctggacgac aggccgacgc gtctgggaag ccccggcgtg ccgatgtatg
    18901 gctataacgt gcagttgctt aatgaagtca ccggcgaacc gtgtggcgtc aacgagaaag
    18961 ggatgctggt ggtggaaggg ccgctgccgc cggggtgtat tcagaccatc tggggcgacg
    19021 acgaccgctt tgtgaagacg tactggtcgc tgttttcccg cccggtgtac gccacctttg
    19081 actggggcat ccgtgacgct gacggttatc actttattct cgggcgcact gacgatgtga
    19141 ttaacgttgc cgggcatcgg ctggggacgc gcgagattga agagagtatc tccagtcatc
    19201 cgggcgttgc cgaagtggcg gtggttgggg tgaaagatgc gctgaaaggg caggtggcgg
    19261 tggcgtttgt cattccgaaa gagagcgaca gtctggaaga tcgtgatgtg gcgcattcgc
    19321 aagagaaggc gattatggcg ctggtggaca gccagattgg caactttggc cgcccggcgc
    19381 acgtctggtt tgtctcgcaa ttgccaaaaa cgcgatccgg aaaaatgctg cgccgcacga
    19441 tccaggcgat ttgcgaagga cgcgatcctg gggatctgac gaccattgat gatcctgcgt
    19501 cgttgaatca gatccgccag gcgatggaag agtaaggtat tgtcggatgc gtcgcgtggc
    19561 gcatccgaca gttgtgcgca gatgcctgat gcgacgc
//
LOCUS       DAZKIP010000079        19272 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZKIP010000079 DAZKIP010000000
VERSION     DAZKIP010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.079"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            40..558
                     /locus_tag="ACHOUK_003113"
     CDS             40..558
                     /locus_tag="ACHOUK_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFP9898506.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            574..1113
                     /locus_tag="ACHOUK_003114"
     CDS             574..1113
                     /locus_tag="ACHOUK_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFP9898507.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1206..2783)
                     /gene="guaA"
                     /locus_tag="ACHOUK_003115"
     CDS             complement(1206..2783)
                     /gene="guaA"
                     /locus_tag="ACHOUK_003115"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFP9898508.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2852..4318)
                     /gene="guaB"
                     /locus_tag="ACHOUK_003116"
     CDS             complement(2852..4318)
                     /gene="guaB"
                     /locus_tag="ACHOUK_003116"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFP9898509.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4480..5850
                     /gene="xseA"
                     /locus_tag="ACHOUK_003117"
     CDS             4480..5850
                     /gene="xseA"
                     /locus_tag="ACHOUK_003117"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFP9898510.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDLQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQCNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGKVLKKVKQVKAGEMLTTRLEDGWVESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5847..6062)
                     /locus_tag="ACHOUK_003118"
     CDS             complement(5847..6062)
                     /locus_tag="ACHOUK_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFP9898511.1"
                     /translation="MELHCPQCQHVLDQDNGHAYCPSCGKVIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6131..7603)
                     /gene="der"
                     /locus_tag="ACHOUK_003119"
     CDS             complement(6131..7603)
                     /gene="der"
                     /locus_tag="ACHOUK_003119"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFP9898512.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7721..8899)
                     /gene="bamB"
                     /locus_tag="ACHOUK_003120"
     CDS             complement(7721..8899)
                     /gene="bamB"
                     /locus_tag="ACHOUK_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFP9898513.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPAVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8910..9530)
                     /gene="yfgM"
                     /locus_tag="ACHOUK_003121"
     CDS             complement(8910..9530)
                     /gene="yfgM"
                     /locus_tag="ACHOUK_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFP9898514.1"
                     /translation="MEIYENENDQVEAIKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9548..10822)
                     /gene="hisS"
                     /locus_tag="ACHOUK_003122"
     CDS             complement(9548..10822)
                     /gene="hisS"
                     /locus_tag="ACHOUK_003122"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFP9898515.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10933..12051)
                     /gene="ispG"
                     /locus_tag="ACHOUK_003123"
                     /gene_synonym="gcpE"
     CDS             complement(10933..12051)
                     /gene="ispG"
                     /locus_tag="ACHOUK_003123"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFP9898516.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12078..13091)
                     /gene="rodZ"
                     /locus_tag="ACHOUK_003124"
     CDS             complement(12078..13091)
                     /gene="rodZ"
                     /locus_tag="ACHOUK_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFP9898517.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPVVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTAATTPDGAAPLPTDQAGVTTPVADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13376..14530)
                     /locus_tag="ACHOUK_003125"
     CDS             complement(13376..14530)
                     /locus_tag="ACHOUK_003125"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFP9898518.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPAAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14680..15111)
                     /gene="ndk"
                     /locus_tag="ACHOUK_003126"
     CDS             complement(14680..15111)
                     /gene="ndk"
                     /locus_tag="ACHOUK_003126"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFP9898519.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15252..16106)
                     /locus_tag="ACHOUK_003127"
     CDS             complement(15252..16106)
                     /locus_tag="ACHOUK_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFP9898520.1"
                     /translation="MLSHNITIGAECARQRFRFATCHACVEACPAQALTLTEEGIAVD
                     AEQCIDCAVCQFICPQEAIRGVNSPLRYFCNHTLVAPFTTIAPTLEELLLWHKEQGIT
                     AIAIDLSQAKAWERALLLLNECLRQYGEEGWSVVSPHTSEVNVSRRRLFHVQRADVQM
                     TQIQPGLRRLHYLWPQISDARPMMDPQKCQLCGACWRACEQQVFSLNEGHLQINDALC
                     NGCQNCIAVCFHQAMTVELTILPAKIVNLHANRKVCKTCQKSFLTFQQNAQNCLYCQR
                     HRYGMRTP"
     gene            complement(16106..16927)
                     /locus_tag="ACHOUK_003128"
     CDS             complement(16106..16927)
                     /locus_tag="ACHOUK_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     family protein"
                     /protein_id="HFP9898521.1"
                     /translation="MHELPLMAFTLLLQASVGMTLWLAFLHRHILITSSARPVMRPPL
                     LLAFLAGAVGLLISTLHLGYPLNAMHALSHFSSSWLSREIIFGALYLALLGLSTLLVM
                     LRKGGWQLLLMLAAVVGIVDVFCMAQVYIHTSIITWQHYNTLIMFLGTVGILGSALVA
                     VFRISGILPQIDALRNGCVLVIALLVLLRLLVQPLWIGDLTANAMQIATLPHAPLAML
                     GQLKPILTLSWGISVIGMMFFAVGGCKKNIPAALFGSVMLVGSEVMLRFVFFSIG"
     gene            complement(16920..17549)
                     /locus_tag="ACHOUK_003129"
     CDS             complement(16920..17549)
                     /locus_tag="ACHOUK_003129"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex B
                     subunit"
                     /protein_id="HFP9898522.1"
                     /translation="MSQFKEFASVSDKQLGFFIDSSRCSGCKACQVACKDKNNLEVGR
                     RFRRVYEVNGGGFIATGEGGVSHNVFAYTLSVSCNHCADPICTKNCPTMAMHKRPGDG
                     IVRVNTDKCVGCGYCAWSCPYGAPQMNEQTGQMSKCDFCIDLQAKGEQPICVATCPLG
                     AIKFGPIDELREKYGDVSDVKGLPDSSITQPNLVIKPHQGAEKEASHHA"
     gene            complement(17546..>19272)
                     /locus_tag="ACHOUK_003130"
     CDS             complement(17546..>19272)
                     /locus_tag="ACHOUK_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
                     /protein_id="HFP9898523.1"
                     /translation="TLLDTKLVILWGHNPTETIFGHTNYYFQKMKQNGTRFIVVDPRY
                     SDTVSSLADQWIPLLPTTDNALMDAMMYVIISENLHDKTFIDTYTLGFDENSMPEGVP
                     ANESLVAYLFGAKDGIHKTPEWAEKITHVPAQSIRQLARDYATTKPAALIQGWGPQRH
                     ICGERTARGSTLLASITGNVGIKGGWAAGYGGSSNRKFCVGPDMPENPVQAKISIMNW
                     MQAADDASKVTPQDGLKGVDKLDSNIRLLFSLAGNYLANQNPDVHQAAKLLEDESKIE
                     FIVLSDLFMTPSAKYADVLLPETSFMERWNIGETWGTASYLILSEKLIEPDFERRTDY
                     DWLRDVAKKLGVEAEFSQGRDEKQWIEHIWEQTRLAMPDENLPDFATLQKTRRHLFKS
                     APHIAFEANIRDPQNNPFPTPSGKIEIFSKRLFDMQDPEIPALSHYVPAFEGPEDKLT
                     AKYPLQLITWKGKNRANSTQYANPWLQEVQTQKLWLNPQDAKQRGISEGDSVKIYNDR
                     GVSIIPVEITPRIIPGVVAMQAGAWWQPDAQGIDRGGCANVLSSTRITALAKGNSHQT
                     MLVEVEKA"
BASE COUNT         4761 a         5234 c         4690 g         4587 t
ORIGIN      
        1 taaatgtctt atttttcgta atgtgtataa caaggaatag tgatgaaatt taaaaaatgt
       61 cttctgcctg tggcaatgtt agcgtcattc actctggcag gatgccagtc aaatgctgac
      121 gatcatgccg ccgatgttta tcaaaccgat caactgaata ccaaacaaga aactaaaacc
      181 gttaatatta tttccattct tcccgcaaaa gttgccgtag acaactccca aaataaacgg
      241 aacgcacaag ccttcggcgc gcttattggc gcagtcgctg gcggtgttat cggccacaac
      301 gtcgggtctg gcagcaattc cggaacgacg gcaggtgcag ttggcggcgg agctgtaggc
      361 gcggcagcgg gttctatggt gaatgataaa accttagtgg aaggtgtttc tttaacgtat
      421 aaggaaggca ccaaagtgta tacctctact caggtgggta aagagtgcca gtttacgaca
      481 ggtttagccg ttgttattac cacgacgtat aacgaaacgc gtattcagcc aaataccaaa
      541 tgtcctgaaa agagctaata atcaggagga gtcatgaaga aagtttttct ttgcgccatc
      601 ttagcctcct taagctatcc ggctatcgcc tcatcattgc aggatcaact ctctgctgtc
      661 gcagaagcgg aacagcaagg taaaaatgaa gagcaaaggc agcatgacga atgggtcgcg
      721 gagcgcaaca gggaaatcca gcaagagaag caacgtcgcg caaatgccca ggctgccgct
      781 aacaaaagag cggcaacggc agcggcaaat aagaaagctc gtcaggataa actggacgcc
      841 gaagccactg cggacaaaaa acgcgatcaa agttatgaag atgagctacg cagcttagag
      901 attcagaaac aaaaactggc gctggcaaaa gaagaagccc gcgttaagcg agaaaacgaa
      961 tttatcgatc aggagctgaa gcacaaagcc gcgcaaactg atgtcgtgca atctgaagct
     1021 gacgccaaca gaaatatgac tgaaggcggt cgcgatctga tgaaaagcgt gggcaaagca
     1081 gaagagaata aatcggacag ctggtttaat taatcgatgt tagtaacttc aagcctataa
     1141 ttcttgaaga taaaaaaccc tctgtagtaa cagagggttt tgttcattca tagtgcaggg
     1201 tcaaatcatt cccactcaat ggtagctggt ggcttgccgc tgatgtcata caccacgcgg
     1261 gaaataccgt tcacttcatt gataatgcgg ttggaaacgc gaccgaggaa atcgtacggc
     1321 agatgcgccc agtgtgcggt cataaagtcg atggtttcga cagcacgcag agagacaacc
     1381 cagtcatact tacgaccatc gcccattacg ccaacggaac gtactggcag gaagacggtg
     1441 aacgcctggc tgactttgtc gtacaggtcc gctttacgca gttcttcaat gaagatggcg
     1501 tcagcacggc gcagcaggtc acagtactct ttcttcactt cacccagaac acgaacgcca
     1561 aggcctggtc ccgggaacgg gtgacggtac agcatgtcgt acggcaggcc cagctccaga
     1621 ccaatcttac gcacttcgtc tttgaacagc tctttcagcg gttcaaccag gcccatcttc
     1681 atctctttcg gcaggccgcc cacgttgtgg tgagatttga tgacgtgtgc tttaccggtt
     1741 gcagacgccg cagattcgat aacgtcaggg tagatggtgc cctgcgccag ccacttcacg
     1801 tcttccagtt tcagcgcttc ttcatcgaat acttcaacga aaacgcgacc gatgatttta
     1861 cgttttgctt ccggatcgtt ttcgccagcc agcgctgaca ggaagcgatc ttctgccggt
     1921 acgtgaacaa tgttaagacc aaagtgatcg ccaaacatat ccagaacctg ctctgcttcg
     1981 ttgagacgca gcaggccgtt gtcgacgaat acgcaagtca ggtttttacc gatagcgcgg
     2041 tgcagcagca ttgcggttac ggaggaatcc acaccaccag agaggccgag gatgacttta
     2101 tcgtcgccta cctgctcgcg gatgcgagct acagcatcgt cgataatttt cgctggcgtc
     2161 cacagggctt cacactggca gatatcacgc acaaaacgct ccagcatgcg cataccctgg
     2221 cgggtgtgag tcacttccgg gtggaactgt acgccataga agcgtttttc ttcgttagcc
     2281 ataatggcaa acgggcagct ttcggtgctg gctacggtga cgaagtcgga cgggatagcg
     2341 gtaactttat cgccgtggct catccagaca tcgagcagcg gtttaccgtc tgcggtcagc
     2401 gcatcttcga taccgcgaac cagtgcgctg tcgtttacga cttcaacctg cgcgtagcca
     2461 aattcacgtt cgttagaggc ttcaacgtga ccgcccaact gcattgccat ggtctgcatg
     2521 ccatagcaaa cgccgaatac cggtacgcct gcttcaaaga catactgcgg cgcacgcggg
     2581 ctgttttctt cagtagtact ttccgggccg ccggaaagaa taatgccgct tggattgaag
     2641 tcacgaattt gtgcttctgt cacatcccat gcccacagtt cgcagtaaac acccagctca
     2701 cgcacgcggc gcgcaaccag ttgagtgtac tgagaaccga agtccagaat gaggatgcga
     2761 tgcttatgaa tgttttccgt cattgacgct tattccgagg caagtgaaac agataatata
     2821 aatcgcccga catgaagtcg ggcgaagaga atcaggagcc cagacggtag ttcggggact
     2881 ctttagtaat ggtcacgtcg tgaacgtggc tttcctgaat gcccgcaccg ctgatacgta
     2941 caaactccgc tttagtacgc agttcgtcga tagtaccaca gccggtcaaa cccatacagg
     3001 agcgcaggcc gcccatctgc tggtgaatga tctctttcag gcgaccttta taagctacgc
     3061 gaccttcgat accttccggc accagtttgt cggcagcgtt atcgctctgg aaataacggt
     3121 cagaggaacc tttggacatc gcgcccaggg aacccatacc acggtaagat ttgtaagaac
     3181 ggccctggta gagttcgatt tcacccggag attcttcagt acccgccagc atggaaccta
     3241 ccatcaccgc gcttgcgcca gcggcgatag ctttggcgat gtcgccggag aagcgaatac
     3301 caccatctgc gataaccgga ataccggtgc cttccagggc ttcaactgcg tcagcaacgg
     3361 cagtgatctg cggaacacct acgccagtaa cgatacgagt cgtacagata gaaccagggc
     3421 cgataccgac tttaaccgcg ctgcaaccag cttctgccag agcgcgtgca cctgcagctg
     3481 ttgccacgtt gccgccgatg atttgcagat ccggatattt agcacgggtt tcacggatac
     3541 gctgcaaaac gccttctgag tgaccgtggg aggagtcgat cagcagaacg tcaacgccag
     3601 cggcaaccag cgcgtcaaca cgctcttcgt tacccgcacc tgcgccaact gcagcaccaa
     3661 cacgcagacg gccttgctcg tctttacagg cgttcggttt acgttccgct ttctggaaat
     3721 ctttcacggt gatcatgccg atcaggtgga attcgtcatc aaccaccagc gctttttcaa
     3781 cgcgtttttc gtgcattttt gccagcacca cttcacgggc ttcaccttca cgcacggtga
     3841 ccagacgctc tttcggcgtc atgtaaacgc taaccggctg gttcaggtcg gtaacaaaac
     3901 gcacgtcacg accggtgata atccccacca gttcgttttc ttcggtaacg accggatagc
     3961 ccgcaaaacc gttacgctcg gtcagttctt tcacttcgcg cagcgtcgtg gttggcagaa
     4021 cagtctgcgg atcagtcacc acaccagatt cgtgtttttt cacacggcga acttcttctg
     4081 cctggcgttc aatggacatg tttttgtgga taaagccgat accgccttcc tgagccagag
     4141 caatagccag gcgcgcttcc gttacggtat ccattgctgc ggaaagcata gggatattca
     4201 gacgaatagt tttcgtcagc tgggtgctga ggtcagcagt attcggcaga acggtagaat
     4261 gagcaggaac gaggagaacg tcgtcaaacg tcagagcttc tttagcgata cgtagcatgg
     4321 gcaatatctc gaccagagtg gttaataaat attgccgcgg cattatacag agcgtaaccg
     4381 attgcatcta cccctttttg caaaaaatgc ttgctatccc cgaagggcgg gttactatcg
     4441 actgaataac ctgctgattt agaatttgat ctcgctcaca tgttaccttc tcaatcccct
     4501 gcaattttta ccgttagtcg cctgaatcaa acggttcgtc tgctgcttga gcatgagatg
     4561 ggacaggttt ggatcagcgg cgaaatctct aatttcacac aaccggcttc cggtcactgg
     4621 tactttacac tcaaagacga caccgcccag gtacgctgcg cgatgttccg caacagcaac
     4681 cgccgggtga ccttccgccc acagcatggg caacaagttt tagttcgcgc caatattacg
     4741 ctctacgagc cgcgcggcga ctaccagata atcgttgaga gtatgcagcc ggccggtgaa
     4801 gggctgctgc aacagaagta cgaacagctc aaagcgaagt tgcaggctga aggattgttc
     4861 gatctgcaat acaaaaaacc acttccctcc cctgcgcatt gcgttggtgt gatcacctca
     4921 aaaaccggtg ctgcgctaca tgatattttg catgtgttaa aacgtcgcga tccttctctg
     4981 ccggtgatca tctaccccac cgccgttcag ggcgatgacg cgccggggca aattgttcgc
     5041 gccattgaac tggcgaatca gtgcaatgag tgcgacgtgt tgatcgttgg gcgcggcggc
     5101 ggttcgctgg aagatttatg gagttttaac gacgaacgcg tagcgcgggc gatttttgcc
     5161 agccgcattc cggtcgtcag cgccgtcggg catgagacgg atgtgaccat tgccgatttt
     5221 gttgccgatc tgcgtgcacc aacaccgtcg gctgccgccg aagtggtgag ccgtaatcag
     5281 caagagttac tgcgccaggt gcaatcgacc cgtcaacggc tggagatggc gatggattat
     5341 tatctcgcca accgcacacg tcgctttacg caaattcatc accgattaca gcaacagcat
     5401 ccgcagctcc ggctggcacg ccagcaaacc atgcttgaac gcctgcaaaa acggatgagc
     5461 tttgcgctgg aaaatcaact taagcgtgcc gggcaacagc agcagcgatt aacacagcgg
     5521 ctgaatcagc aaaatccaca gccgaagatt catcgcgcgc aaacgcgcat tcagcaactg
     5581 gaatatcgtt tagcagaaac cctgcgcgca cagcttagcg ccacgcgtga acgtttcggt
     5641 aatgcagtaa cgcacctcga agccgtaagc ccactgtcaa cgctggcgcg tggatacagc
     5701 gttactactg ctactgacgg caaggtactg aaaaaagtga agcaggttaa agcgggtgaa
     5761 atgctaacca cacgtctgga agacggctgg gtagaaagtg aagtaaaaaa catccagccg
     5821 gtaaaaaaat cgcgtaaaaa agtgcattaa gccagcacga attcaacgcg ttttttcgat
     5881 atcaagccat gcccgtgctg acagaagtaa tctaccgcac cgcaggcttt taacacctgc
     5941 aacggttgat ggcagtccgg gcaaagagct ttcatttcaa taactttccc gcaggatggg
     6001 caatacgcat ggccgttatc ctgatcaagc acatgctgac attgtggaca atgcagttcc
     6061 atatgaattc ctccggtagc cacccctcta cattcataga gggaatggca gataaaatac
     6121 ttacggataa ttatttattt ttcttgatgt gcttcatcag acgcttacgt ttacgcatct
     6181 gggttggcgt cagggtgtta cgcttgttcg catacgggtt ttccccttct ttgaactgaa
     6241 tacgaatcgg cgatcccatt acgtccagcg atttgcggaa gtagttcatc aagtaacgct
     6301 tgtaggaatc aggcaggtct ttcacctgat taccgtgaat caccacaatc ggcggattat
     6361 aaccaccggc gtgtgcatat ttcagcttca cacgacgacc gcgtaccagc ggcggttggt
     6421 gatcttcaac agccatcgtc atgatgcgcg tcagcataga ggtccccaca cgacgggtgg
     6481 agctgtcata cgcttcacgt actgattcaa acaagttacc aacaccactg ccgtgcaagg
     6541 cagagataaa gtgcacacga gcaaaatcga taaagcccag acggaagtcc agcgtttctt
     6601 tcacctgctc tttcacttcc tgactcaggc catcccactt attcaccaca atgacaagtg
     6661 agcgcccact attgagaata aagcccagca gcgagagatc ctgatcggaa ataccttcgc
     6721 gcgcatcaat cactaacatc accacgttgg cgtcttcaat ggcctgcaac gttttgatta
     6781 cggagaattt ctctacagca tcggtgattt tgccgcgttt acgtacgcca gcggtgtcaa
     6841 tgagcacata ctcacgtcca tcgcgttcca ttggaatgta gatactgtca cgcgtcgtac
     6901 caggcatgtc ataaacaaca acgcgctctt caccaagaat acggttagtg agtgtagact
     6961 tacctacgtt cggacgaccc acaatcgcca gtttgatcgg cagactttgc gggtcgaagt
     7021 cgtcttcctc ttcttcttcg ccgttctctt ccgcttcaaa ttgcgcccag tattcagcgt
     7081 cttcgtcgac ttcctcttgc ggtgcgagat cttccatcca cggcagtagc acatgctcca
     7141 gcagacttaa tacgccacga ccgtgagacg cggcgatcgg gtagatttca cctaaaccaa
     7201 gcgagtagaa atcaaccact gcctgatcgg gatccagacc gtcagttttg tttgccacca
     7261 ggaaggtcgg tttttcacgg gagcgcagat gtttggcaat cgcttcatct gccggcatca
     7321 ggcccgcgcg cgcatccacc ataaacagta cgacgtccgc ttcttcaatc gccagcagcg
     7381 actgttccgc catgcgggtt tctacaccgt cttctgtgcc atcaatcccg ccggtatcaa
     7441 tacagataaa ctcacggcct tcaatttccg cacgaccgta cttacggtca cgagtcagac
     7501 ccgggaaatc cgcaaccagc gcatctcggg tgcgagttag acggttaaat aacgtggatt
     7561 ttcctacgtt agggcgcccg acaagcgcga ccacaggtac catgtttaaa gcctcatttt
     7621 tataaatcat cagacaacgc acgctatatt cgcgtcgttg ttaaaaacag gaaaacggcc
     7681 cctgtccagg agccgttttc aaagtgaacg acagagacga ttaacgtgta atagagtaca
     7741 cggttccgtc ttttgcctgg atcagcagtt tgccgtcagc ggcaaccggt tcagtctgga
     7801 aaccggaact atcaactttt tgctgggcaa cgaaacgacc atcttcgacg ttaatccagt
     7861 gcaaataacc ttcactgtca ccgaccacca ggttgccatt atacagcacc ggagaagtca
     7921 gcaggcgatg cagcagatcg ctttgtgtcc acagcgtaac gccgccatca atggtcaacg
     7981 ccatcacccg gtcattttga tcgaccagat agatgcgatt gccgtcgacg atgaaatcat
     8041 tcaccgaacc cagttcgcgt ttccacataa tctgaccact gcgcagatca agcgccgtca
     8101 ggttaccatt ataggccagc gcgaaaacaa cgccgttaac aacggcggga gtcgtgtcaa
     8161 catcgctcag acggtcaatt tcggtagaac cggtcgcttg ggaaatacgc tgctgccaaa
     8221 tcatctggcc ctgttccatc agcactgcgc tgacgcgacc attatcgccc cccacgacgg
     8281 ccgcaccaaa agccgttgcc ggcgcagact cgccacgcaa agagagcgaa ggcatatcga
     8341 ggttaactgt ccatttgaca gcgccgtcag cttcgttcag cgcttgtaac tgaccgttac
     8401 tggtgtggat taacaccaga ccgtcgctga ccaccgggcg cgaaagtgct tcacccgcga
     8461 ctttagtttg ccatgccaca gtaccatcgc tggtattcag cgcgtaaacc tgcgcctttt
     8521 cgctgccaat gtagacatgt ccaccagaca cggtcacacc gccagaaagt aatgcaggct
     8581 ctttagagaa ccagccatct ttctcggcca ggctgacaga ccagatttct ttgccatcat
     8641 ccgcattcag cgcttttact aaaccagcgc ggtccgctgc atagacaacg ttgtccgcca
     8701 gtgccggatg aagattggaa tagaagttgc caatgccgct accaacggaa gtgctccacg
     8761 ccgtggtcgg cgtaaactgg ttttcaacgg ttggcaatgg ggacatcttt accacatctt
     8821 cttcgctgtt aaacagcgaa cagccgctta aaagggtaac ggaaagcagt cctggcagca
     8881 gtaatttacg caattgcatc gggtccctct cagatggaca aattattaat tttcatctgc
     8941 atcatttcgc tcagtgccgg agtaacatcg cttttcacgc ctgcttccca tgcactacgc
     9001 gcaccttgct tatcaccttt gctcagcaat gcttcaccac gcaggtcggc aacaatggca
     9061 gcccaccctt cacctttgat ggtatcaagg gttttcagcg cggcatcagc ctgcttgagc
     9121 tgtacctgaa cgcgagcaag acgcagattt atcacggctt tgagattttc atcgctcgtg
     9181 tctgccagcc cctgttgtaa ctgggcggca gctttctcta gttcattttt gtcaacaaat
     9241 tgctgcgcaa gttccaaaga agccagcgca ccataagtat ttttattttc agcagcaaat
     9301 ttttccgccg ccgggatgct atccggtttg ccttcgctca ctgcggtaac cgcattttga
     9361 taggcaagag aagcggagcg tgcagaatca acctgatggc tgttccagta gcgccagcca
     9421 atcagtgcgc caacgcccaa aatcacccca acagccagtg ctttgccatt ttcagcaaaa
     9481 aagcgtttaa tcgcttctac ctggtcgttt tcgttctcgt aaatttccac gctgtccttc
     9541 tccttcctta acccagtaac gtgcgcaaat gcgcggctac gctatcctgc gcaactgccg
     9601 tttgctcacc agagcgcaaa tccttcacta ctgctgtgcc gttagccact tcagactcac
     9661 ccagcaccac agcaacgcgg gcaccccatt tatcagcacg ggcaaactgt ttcttaaagt
     9721 tgccgccgcc gtggttggtc atcaatttca cgcccggtaa ttcatcacgc agacgctcag
     9781 ctaatgccat agccgcagat tgtgtatcag cacctgaagc caccaggtat atatcgacaa
     9841 caggatcggc tttaaattcc ggattaacgg cctgtactaa caatacaaga cgttcgaggc
     9901 ccatcgcaaa accgacagcc ggtgttgcac ggccgcccag ttgttccaca agaccgtcat
     9961 aacgaccgcc tgcacacacg gtgccctggg agccgagact gttagtcacc cactcgaaaa
    10021 cggtacggtt gtagtaatcc agaccacgca ccagacgctg gtttacggtg taagcgatcc
    10081 ccgcgctctc aagcagtttg cacagaccgg caaaatgctc acgagattcc tcgtccagat
    10141 aatcacctaa tgccggagcg tcgttgagaa gcgcctgcac ttccggattt tttgaatcca
    10201 gcacgcgcag cgggttagtg tacatgcggc gtttgcagtc ttcgtccagc ttttctttat
    10261 gctgctcaag gaatgccacc agcgcatcgc ggtaattggc gcgtgcttcc agcgaaccga
    10321 tagaattcag ctcaagagtt acgtgttcgg aaatacccag cgcgcgccac cagcgggcgg
    10381 tgagcataat cagttcagcg tcgatatccg gaccttgcag accgaaaact tcgcagccca
    10441 actgatggaa ctgacgataa cgccctttct gcggacgctc gtgacggaac atcggcccga
    10501 tataccacag acgctgttcc tgattgtaca gaagaccatg ctcgatgccg gcgcgtacac
    10561 agcccgccgt cccttcaggg cgcagagtca ggctgtcgcc attgcgatcc tcaaaggtgt
    10621 acatctcttt ttcaaccacg tcggtgactt caccaatcgc acgtttgaat agcggggtct
    10681 gctctacaat cggcaagcgg atttcactgt aaccgtagct gccgagcacg tttttcagtg
    10741 tgccttcaat gcgctgccag atggccgttt cgccaggcag gtaatcgttc atgccgcgaa
    10801 tggcttgaat gttttttgcc acgtttattc tctttctgaa tataaaaatg aaccctcaac
    10861 gcttccctca atgtttcggg agccatgcgg gttcaatcat acacgggaag cgaggcgctt
    10921 cccatcacgt tattattttt caacctgctg aacgtcaatt cgacgcgctt cgtccagctg
    10981 actggctttc gcacgaatgc gtgcctccag ctggtcgatc atatcgttgt tgtccagacg
    11041 gtctttgcgc acgccatctt catagagacc gcttttcttg ttgccgccgg tgacgccgag
    11101 tgtagaaacc agcgcctcac ccgggccatt caccacgcag ccgataatcg aaacgtccat
    11161 cggagtgatg atatcttcca ggcgttgctc cagcgcgtta actgtaccga taacatcaaa
    11221 ttcctgacgc gaacaggtcg gacaggcgat gaagttgata cctcgcgaac ggatacgaag
    11281 cgatttcaaa atatcgaaac cgactttgat ctcttcgacc ggatcggccg ccagtgatac
    11341 gcgcagcgtg tcgccgatgc cttcagacag cagcagacct aaaccaatgg cggattttac
    11401 tgccccgctg cgcgcgccac ccgcttcggt gatccccaga tgcagcggct gatcgatttg
    11461 ttttgccagc aaacgataag actcaacagc gaggaagacg tcagacgctt tcacgctgac
    11521 tttgaactga tcgaagttca ggcgatcgag atgatcaaca tggcgcatgg cggattccag
    11581 caacgcctgc ggcgtcggtt cgccatactt ttcttgcaga tctttttcca gcgatccggc
    11641 gttaacgcca atacggatcg gaatgttttt atcgcgcgca cagtcaacca ccatgcgaat
    11701 acgctcttca ttaccgatat tgccagggtt aatacgcaga caatcgacgc cgtattccgc
    11761 tactttcagc gcaatgcgat agtcgaagtg gatgtcagcc accagcggca cgttaacctg
    11821 ctgtttgatg agtttgaacg cttctgccgc gtccatcgtc ggtacggaga cacggacgat
    11881 atcagcgcca acgcgttcca gcgccttgat ttgattgacc gttgcttcga catctgtcgt
    11941 acgcgtattg gtcatggact gtacggcgat gggagcacca tcgccaatcg gcacattccc
    12001 aacgtaaata cgtgttgatt ttctacgttg aattggagcc tggttatgca tgaaaaatct
    12061 cccgcgttac ccgtctgtta ctgcgccggt gattgttcgg cattgagggt cagacgcgca
    12121 acctggttag ttctgataaa acgactcaga tcgacaggtt tcccttgata ctggatctgt
    12181 actgcggctg gcgcaccaat tttcagtttg tacggtgcct ggccggttaa gttcaaatta
    12241 ccgtctttac gctgcatacc gctaaacaat tttttaccgg tagcatcagt gacctccagc
    12301 cagcaatcgg cagtaaagtt catcaccagc gcattcggat cagccaccgg cgtggtcacg
    12361 ccagcctgat cggttggcaa cggcgcagca ccatctggcg ttgttgctgc cgttggtgcc
    12421 ggggtcgcgg cggtatcaac atttgcctgc gaaggcgaaa caaccgcatt ctgttgcgga
    12481 tcaacagctg gtgctggcgc agttacgaca ggtgtttgcg tgttggttgc ggtagtatcc
    12541 acagacgctg gcggcgtgga cgttgtagcc gggtctgtag ttgtcgacgt atttaacgga
    12601 acactctgcc cctgctcgct attactgctc agttccgccg aagattgatc ggccatagtg
    12661 gtgatctctt cctgctgagc tttgtggtct tgccaccacc aggcaccgct caggccgata
    12721 accacaaaca acaccagcca ggtgaaggtc atcagccagc cgtcgcgttt tttgcggcgt
    12781 ttaccgaggg aaaaactctg catcggcgca acttttgcag cccgtagtgg agcctgcttt
    12841 tccagccctg gcagcagttc ttcttctgga atatgtacca gacgcgcata agagcggata
    12901 tatccgcgca ggaatgttga agcaagatcg gcgggtgcct tatcttcttc aatgtcgcgt
    12961 accgtggaaa ccttcaggca aagtcgctcg gcaacggcct gctgactaag tcctagttgt
    13021 tcgcgagcat tacgcaggcg agcgccggta gtaagtgctt cattttggtc gtgcgtggct
    13081 tcagtattca ttcgctgcag gtacgtttaa atgagaattg agatgccggt gaaccatcat
    13141 gctcacccac accgcgaaac atccgttaag ttaaccgtta tcatacagta taagaccgtc
    13201 tgtgtgctcg tgacaaagcc cacattcact aaggctaact tactgttgca tcgttacata
    13261 ctgccttaaa gtcagcaaaa acgcaccgtt aatattgacc agacaattgc aacttaatac
    13321 ttcattcata aaacattacg ccacggtaca taaagtaacc gtggcgtaat ggctatcaga
    13381 ccgctttaat gtcgatggct tcaccctgca tccgtttacg cagggtacgt ttcgtacggt
    13441 cgataacatc gcccgccaac tgaccacagg cagcatcgat atcatcacca cgcgttttac
    13501 gcacaatagt ggtgaaaccg tagctcatca gcacttttga gaaacggtcg atacggctgt
    13561 tcgagctgcg tccatacggc gcagccggga acgggttcca cgggatcagg ttgatcttac
    13621 acggcgtatc tttcagcagt tccgccagtt ggtgcgcgtg ttcagtgccg tcgttaacgt
    13681 ggtcaagcat cacgtattca atagtgactc ggccctgatt ggcgttggat ttctccagat
    13741 aacggcgcac cgcagcaagg aacgtttcga tattgtactt tttgttgatc ggcacaattt
    13801 cgtcacgaat ttcgtcgttc ggcgcgtgca gggaaattgc cagcgcaaca tcgatcatat
    13861 cgcccagttt atccagcgct ggaactacac cggaagtgga aagcgtcacg cgacgtttag
    13921 acagaccaaa accgaaatca tcaagcatga tttccatcgc cggaacgacg ttgttcaggt
    13981 tgagcagcgg ctcgcccatg cccatcatca ctacgttagt gattgggcgc tgaccggtga
    14041 cttttgctgc gccgacgatt ttcgccgcac gccacacctg gccaataatt tccgataccc
    14101 gcaggttgcg gttaaagccc tgctgggcgg tggaacagaa tttacactcc agcgcacacc
    14161 ccacctgcga agagacgcag agcgtagcgc ggtcgtcttc cgggatgtac accgtttcga
    14221 cacgctggtc acccacggca atcgcccatt taatggtgcc gtcagatgaa cgctgttctt
    14281 caaccacttc cggtgcgcgg atctccgcca cctctttcag tttgccgcgc aacactttgt
    14341 tgatgtcggt catctcatca aaattgtcgc agcaatagtg atacatccac ttcatcacct
    14401 gatcggcgcg gaagggtttt tcacctaaat ctttaaaaaa ctcccgcatc tgctgacgat
    14461 tgagatccag caggttgatt tttccatctt tggtggtgac gttttcaggt gtgactaatt
    14521 gttcagacat atgctattcc ggcctcgtta ttacacgtta tggcccctgg agggttgaaa
    14581 aaagaaacgc cccggtgagc ggctgctcgt ccgggggcgc tgcattgtac aaattctggc
    14641 gcacggatgc cacgtttgca cgcggcattt acgaaattat taacgggtgc gcgggcacac
    14701 ttcgccttcg ccgaagaaat aagcgatttc gcgagcggca gattcgacgg aatcagaacc
    14761 gtgggtaccg ttttcggtca ggctgtcagc gtaatcagcg cgcagagtac cggccagtgc
    14821 gtttgccgga ttggtcgcgc ccagcagatc gcggtgacgc tgaacggcgt tttcaccttc
    14881 cagcacggaa accacgatcg ggccagaggt catgaattca accagaccat caaagaacgg
    14941 ttttccatcg tgttcagcat aaaagccacg tgcctgttca acggtcaggt gcagcatttt
    15001 ggtgccgaca attttgaacc ctgcagcttc aaagcgcgca aagatattac caatgacgtt
    15061 ttttgctacc gcgttcggtt tgataatgga aaaagtacgt tcaatagcca tttttacctc
    15121 tgtaaattgt tctgttgttg tctgtaccag cgtacgagat ggcgcggatt ataatgagca
    15181 acagagccgt tgactattga tgaaggtaac atttttttaa aataaaatga gttttagcaa
    15241 caactcgact tttatggtgt tctcatccca taacgatgac gttggcaata aagacaattt
    15301 tgcgcatttt gctgaaaagt aagaaaggat ttttgacagg ttttacagac tttgcgatta
    15361 gcatgaagat tcacaatttt tgccggtaga atcgtcaatt caacggtcat tgcctgatga
    15421 aaacaaacag caatacaatt ttggcatccg ttacacaagg catcatttat ttgtaaatgg
    15481 ccttcgttaa gcgaaaagac ctgttgctcg caggcccgcc agcatgcacc gcaaagctga
    15541 cacttttgag gatccatcat cgggcgagca tcactaatct ggggccataa ataatgtaac
    15601 cgacgtaatc ctggttgtat ttgtgtcatc tgaacatccg cacgctgcac atggaacaaa
    15661 cgacgccggg aaacattcac ctcactggta tgtggagata ccacagacca gccttcctcg
    15721 ccatattgcc gtaagcattc gtttaacagc aataatgcgc gctcccatgc cttcgcctga
    15781 ctcagatcaa tcgcgatcgc tgtgatcccc tgctctttat gccatagcaa aagttcttcc
    15841 agcgtcggcg caatagtggt gaaaggtgcg accagggtgt gattgcaaaa ataacgtaaa
    15901 ggggaattaa cgccacgaat agcctcttgc ggacaaataa actggcaaac cgcacaatca
    15961 atacattgtt ccgcgtcgac cgctatgccc tcttctgtca gcgttagcgc ctgtgccggg
    16021 caagcctcga cacaggcatg acatgttgca aaacgaaagc gttggcgcgc gcattccgcg
    16081 ccaatcgtta tgttatgact taacattagc cgatactaaa aaagacaaaa cgcagcataa
    16141 cttcactccc cactagcatt acgctaccga ataacgccgc cgggatattc tttttacaac
    16201 caccgacagc aaagaacatc atcccaatga cggaaattcc ccagcttagt gtcagtatgg
    16261 gttttaactg tcccagcatc gctaatggcg catgtggcaa tgtggctatc tgcatcgcat
    16321 ttgccgttaa atcccctatc cacaacggct gaaccaacaa acgcagcaga accaggagag
    16381 cgatcaccaa cacgcatcca ttacgcaggg catcaatctg cggcaaaatt ccgctaatac
    16441 gaaacactgc taccagcgct gaaccgagaa ttcccactgt acctaagaac ataatcagcg
    16501 tgttgtaatg ctgccaggtg ataatcgacg tatggatgta aacttgcgcc atgcagaaaa
    16561 catcaacaat cccgaccact gccgctagca taagcaggag ttgccagccg cctttgcgta
    16621 acatgaccag taatgtggat aaacccaata gcgccagata cagcgcacca aagataattt
    16681 cccgactcag ccatgaactg gaaaagtggc tcagcgcatg catcgcatta agtggataac
    16741 ccaggtgtaa ggtagaaatc agtaacccta cggcacctgc caaaaaagcc aacagtaatg
    16801 gcggccgcat aaccgggcgg gcgctggagg tgattaaaat atgacgatga agaaacgcca
    16861 gccacaacgt cataccgacc gatgcctgta acaacagcgt aaaagccatt aacggtaatt
    16921 catgcatgat gtgacgcctc cttttccgcg ccctgatgcg gtttaatcac cagatttggc
    16981 tgcgtaatcg acgaatcagg taaacctttc acatcactga catcgccata cttttcacgc
    17041 aactcatcaa tagggccgaa cttaatcgcc cccaacgggc acgttgccac gcaaatgggt
    17101 tgttctcctt tagcttgcag atcaatacag aaatcgcatt tcgacatctg cccggtctgc
    17161 tcattcattt gtggtgcgcc gtaaggacag gaccaggcgc agtaaccgca accgacgcac
    17221 ttatcagtat tcacgcgcac aataccgtct ccgggacgct tatgcatcgc catcgtcggg
    17281 caatttttgg tacagatcgg atccgcgcaa tggttacagg aaacagaaag ggtataagca
    17341 aagacattat gactcacacc gccctctcca gtggcgatga agccacctcc gttcacttca
    17401 tacacgcggc gaaaacgtcg cccaacctca aggttgtttt tgtctttaca ggccacctga
    17461 cacgctttac agcccgaaca acgcgaagag tcaataaaga accctaattg tttgtcgctg
    17521 acagatgcaa attctttaaa ttggctcatg ctttctcaac ctccaccagc attgtttgat
    17581 gcgaattccc tttcgccagc gccgtaatgc gggtcgaact cagtacattg gcacagcctc
    17641 cgcgatcgat accttgtgca tccggttgcc accatgcccc cgcctgcata gcaactacgc
    17701 cagggatgat acggggggtt atttccaccg ggataatgct cacaccacga tcgttataaa
    17761 tttttacgct atctccttcg ctaatcccgc gctgttttgc atcttgcgga ttcagccaga
    17821 gtttttgcgt ctgaacttct tgcagccacg ggttagcata ctgggtggaa ttagcacggt
    17881 ttttcccttt ccaggtgatg agctgcaacg ggtatttcgc cgtcagttta tcttctggcc
    17941 cttcaaaggc agggacatag tgcgaaagcg ccggaatttc tggatcttgc atatcaaaaa
    18001 gacgcttaga gaagatctca attttgccgg aaggcgtcgg gaaaggatta ttctgcggat
    18061 cgcgaatatt tgcttcgaaa gcgatgtgcg gcgcgctttt gaaaagatgt cgacgtgttt
    18121 tttgcagggt tgcgaagtcc ggcaaatttt catccggcat cgccagacga gtttgctccc
    18181 agatatgctc gatccattgc ttctcatcac gtccttgact gaactcggct tcgacaccca
    18241 gttttttcgc cacgtcacgc aaccagtcat aatcggtgcg acgttcaaaa tccggctcaa
    18301 tcagtttttc tgacagaata agatagctcg cagttcccca tgtttcgccg atattccagc
    18361 gttccataaa gctggtttcc ggcagcaaca catcggcata tttcgcgctc ggagtcatga
    18421 acagatcgct gagtacgatg aattctattt tagattcgtc ttcaaggagc ttcgcggcct
    18481 ggtggacgtc cgggttctgg ttcgccagat aattacctgc cagtgagaac agcaggcgga
    18541 tattactgtc gagtttatcg acacctttaa gcccatcttg cggcgtaact ttagaagcat
    18601 catccgccgc ctgcatccag ttcataatcg agattttcgc ttgtaccgga ttttctggca
    18661 tatctggtcc aacgcagaat ttacgattag aactaccgcc gtaacctgcc gcccagccac
    18721 ctttgatgcc gacgttaccg gtaatcgagg ccagtaaagt tgaacctcgg gcggtgcgtt
    18781 cgccgcaaat atgtcgctgt ggcccccatc cctgaatcaa cgctgcgggt tttgtagtgg
    18841 cgtaatcgcg tgccagttga cgaatggatt gtgctggaac gtgggtaatt ttttctgccc
    18901 attccggcgt tttgtgtatc ccatcttttg cgccaaaaag gtaggcaacc aacgattcgt
    18961 tggctggtac gccttccggc atggagtttt catcaaaacc gagagtatat gtgtcgataa
    19021 atgtcttatc gtggagattc tcgctgataa tgacgtacat catcgcgtcc atcaacgcat
    19081 tgtccgtggt cggcaacagc gggatccact gatcggcaag tgaagatacg gtgtcggaat
    19141 agcgcggatc caccacgata aaacgtgtac cgttttgctt cattttttga aaatagtaat
    19201 tggtgtgacc gaatatggtt tccgttgggt tatgccccca gagaatcacc agtttggtat
    19261 ccagaagagt at
//
LOCUS       DAZKIP010000080        19081 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZKIP010000080 DAZKIP010000000
VERSION     DAZKIP010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.080"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(177..1826)
                     /gene="pgi"
                     /locus_tag="ACHOUK_003131"
     CDS             complement(177..1826)
                     /gene="pgi"
                     /locus_tag="ACHOUK_003131"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFP9898524.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      2063..2259
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2351..3700
                     /gene="lysC"
                     /locus_tag="ACHOUK_003132"
     CDS             2351..3700
                     /gene="lysC"
                     /locus_tag="ACHOUK_003132"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFP9898525.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            3953..4225
                     /locus_tag="ACHOUK_003133"
     CDS             3953..4225
                     /locus_tag="ACHOUK_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFP9898526.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4226..5098)
                     /gene="rluF"
                     /locus_tag="ACHOUK_003134"
     CDS             complement(4226..5098)
                     /gene="rluF"
                     /locus_tag="ACHOUK_003134"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFP9898527.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            5446..6393
                     /locus_tag="ACHOUK_003135"
     CDS             5446..6393
                     /locus_tag="ACHOUK_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313031.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFP9898528.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTNISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGEMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            6463..7269
                     /locus_tag="ACHOUK_003136"
     CDS             6463..7269
                     /locus_tag="ACHOUK_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9898529.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEIADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            7279..7686
                     /locus_tag="ACHOUK_003137"
     CDS             7279..7686
                     /locus_tag="ACHOUK_003137"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFP9898530.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQNEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            7686..8180
                     /locus_tag="ACHOUK_003138"
     CDS             7686..8180
                     /locus_tag="ACHOUK_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFP9898531.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIYKINEQ
                     LIAN"
     gene            8246..9043
                     /locus_tag="ACHOUK_003139"
     CDS             8246..9043
                     /locus_tag="ACHOUK_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFP9898532.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLLAPASTALDQLDD"
     gene            9054..9878
                     /locus_tag="ACHOUK_003140"
     CDS             9054..9878
                     /locus_tag="ACHOUK_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP9898533.1"
                     /translation="MEQRKITLGDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPVKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            9930..11159
                     /gene="sorE"
                     /locus_tag="ACHOUK_003141"
     CDS             9930..11159
                     /gene="sorE"
                     /locus_tag="ACHOUK_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFP9898534.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRMLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            11542..12231
                     /gene="pepE"
                     /locus_tag="ACHOUK_003142"
     CDS             11542..12231
                     /gene="pepE"
                     /locus_tag="ACHOUK_003142"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFP9898535.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(12322..13953)
                     /locus_tag="ACHOUK_003143"
     CDS             complement(12322..13953)
                     /locus_tag="ACHOUK_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFP9898536.1"
                     /translation="MLTLLNLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLAMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMSSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(14173..17856)
                     /gene="metH"
                     /locus_tag="ACHOUK_003144"
     CDS             complement(14173..17856)
                     /gene="metH"
                     /locus_tag="ACHOUK_003144"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFP9898537.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWEVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDIANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            18056..18880
                     /gene="iclR"
                     /locus_tag="ACHOUK_003145"
     CDS             18056..18880
                     /gene="iclR"
                     /locus_tag="ACHOUK_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFP9898538.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         4495 a         4974 c         5015 g         4597 t
ORIGIN      
        1 gtttttaagg ttatttaatt aatcgtgcta tgcattaatt gccgaatacg atgtacatat
       61 cggcatcgac ctgtaggcct gataagacgc gtaaagcgtc gcatcaggca ccagttgccg
      121 gatgcggcgt aaacgcctta tccggcctac atagcggcat caggcatgaa cgatgattaa
      181 ccgcgccacg ctttatagcg gttaatcaga ccattggtcg agctatcgtg gctgctgatt
      241 tctttatcat ctttcagctc tggcagaata cggttcgcca gctgtttacc cagttccacg
      301 ccccactggt cgaaggtgaa gatgttcagg atcacgccct gagtaaagat tttgtgctca
      361 tacagcgcaa tcaacgcacc caggctgaac ggagtgattt cgcgcaacag gatagagttg
      421 gtcgggcggt taccttcgaa cactttgaac ggcaccacgt agtcaagcgt tgccggatct
      481 ttaccctgat cgcgatattc ctgctcaacc acttcgcggg atttaccaaa cgccagcgct
      541 tcggtctggg cgaagaagtt agacagcagt ttctggtggt gatcagagag cgggttatgg
      601 gtgatagccg gagcgatgaa atcgcacggt accattttgg ttccctggtg gatcagctgg
      661 tagaacgcgt gctgaccgtt agtgcctggt tcaccccaga taatcgggcc agtctggtaa
      721 tccacaacgt taccgttacg gtcaacatac ttaccgttgg actccatatt gccctgctgg
      781 aagtacgccg cgaaacggtg catatactgg tcatacggca gaatcgcttc agtttccgca
      841 ccaaagaaat tgttgtacca gatgccaatc agcgccaaca gtacaggcag gtttttctcg
      901 gcaggcgtgg tggagaaatg cttgtccatc gcgtgtgcgc cagaaagcag ttcaacgaag
      961 ttatcaaagc cgatggagag aacaatcgac aggccaatcg ctgaccacaa agagtaacga
     1021 ccgccgaccc agtcccagaa ctcgaacatg ttggcagtat caataccaaa ctcgccaacg
     1081 gctttggcat tggttgagag cgccgcaaag tgtttcgcaa cgtgtttttc atcacctgcc
     1141 gctttcagga accagtcacg cgcgctatgg gcgttggtca tggtttcctg agtggtgaag
     1201 gttttagatg ctaccaggaa cagcgtggtt tccgggttta cttttttcag cacttcagcg
     1261 atgtgagtcc catcgacgtt agaaacaaag tgcatgttca ggtgattttt gtacggacgc
     1321 agtgcttcgg tcaccatgta cgggccgagg tcagaaccgc cgatcccgat gttcactacg
     1381 tcagtgattg ctttgccggt ataacccttc cactcaccgg agataatcgc ttccgagaag
     1441 gttttcatct tctccagcac cgcgttgact tctggcataa catctttgcc atcaaccaga
     1501 atcggggtat tgctacgatt acgcagcgct acgtgcagca cggcgcggtt ttcagtgcgg
     1561 ttgatcttct cgccagagaa catcgactta atcgcgcccg ccagatcgca ctctttcgcc
     1621 agatcttgta atttcgccag cgtctcttca gtgatgcggt ttttggagta atccaccagc
     1681 atctgatcgt cgaaggttgc ggagaactta gaaaaacgat cgccgtcttt agcaaaaaga
     1741 tcggcgatcg taacgtcttt catttcatcg aagtgtttct gtagtgcctg ccaggcagcg
     1801 gtctgcgttg gattgatgtt tttcattagc aatactcttc tgattttgag aattgtgact
     1861 ttggaagatt gtagcgccag tcacagaaaa atgtgatggt tttagtgccg ttagcgtaat
     1921 gttgagtgta aacccttagc gcagtgaagc atttattagc tgaactactg accgccagga
     1981 gtggatgaaa aatccgcatg accccgtcgt tgacaaccgc cccgctcacc ctttatttat
     2041 aaatgtacta cctgcgctag cgcaggccag aagaggcgcg ttgcccaagt aacggtgttg
     2101 gaggagccag tcctgtgata acacctgagg gggtgcatcg ccgaggtgat tgaacggctg
     2161 gccacgttca tcatcggcta caggggctga atcccctggg ttgtcaccag aagcgttcgc
     2221 agtcgggcgt ttcgcaagtg gtggagcact tctgggtgaa aatagtagcg aagtatcgct
     2281 ctgcgcccac ccgtcttccg ctcttccctt gtgccaaggc tgaaaatgga tcccctgaca
     2341 cgaggtagtt atgtctgaaa ttgttgtctc caaatttggc ggtaccagcg tagctgattt
     2401 tgacgccatg aaccgcagcg ctgatattgt gctttctgat gccaacgtgc gtttagttgt
     2461 cctctcggct tctgctggta tcactaatct gctggtcgct ttagcggaag gactggaacc
     2521 tggcgagcga ttcgaaaaac tcgacgctat tcgcaacatc cagtttgcca ttctggaacg
     2581 tctgcgttac ccgaacgtta tccgtgaaga gattgaacgt ctgctggaga acattactgt
     2641 tctggcagaa gcggcggcgc tggcaacgtc tccggcgctg acagatgaac tggtcagcca
     2701 cggcgagctg atgtcgaccc tgctgtttgt cgagatcctg cgcgaacgcg atgttcaggc
     2761 acagtggttt gatgtacgta aagtgatgcg taccaacgac cgatttggtc gtgcagagcc
     2821 agatgtagcc gcgctggcgg aactggccgc gctgcagctg ctcccacgtc tcaatgaagg
     2881 cttagtgatc acccagggat ttatcggtag cgaaaataaa ggtcgtacaa cgacgcttgg
     2941 ccgtggaggc agcgattata cggcagcctt gctggcggag gctttacacg catctcgtgt
     3001 tgatatctgg accgacgtcc cgggcatcta caccaccgat ccacgcgtag tttccgcagc
     3061 aaaacgcatt gatgaaatcg cgtttgccga agcggcagag atggcaactt ttggtgcaaa
     3121 agtactgcat ccggcaacgt tgctacccgc agtacgcagc gatatcccag tctttgtcgg
     3181 ctccagcaaa gacccacgcg caggtggtac gctggtgtgc aataaaactg aaaatccgcc
     3241 gctgttccgc gcgctggcgc ttcgtcgcaa tcagactctg ctcactttgc acagcctgaa
     3301 tatgctgcat tctcgcggtt tcctcgcgga agttttcggc atcctcgcgc ggcataatat
     3361 ttcggtagac ttaatcacca cgtcagaagt gagcgtggca ttaacccttg ataccaccgg
     3421 ttcaacctcc actggcgata cgttgctgac gcaatctctg ctgatggagc tttccgcact
     3481 gtgccgggtg gaggtggaag aaggtctggc gctggtcgcg ttgattggca atgacctgtc
     3541 aaaagcctgc ggcgttggca aagaggtatt cggcgtactg gaaccgttca acattcgcat
     3601 gatttgttac ggcgcatcca gccataacct gtgcttcctg gtgcccggcg aagatgccga
     3661 gcaggtggtg caaaaactgc atagtaattt gtttgagtaa atactgtatg gcctggaagc
     3721 tatatttcgg gccgtattga ttttcttgtc actatgctca tcaataaacg agcctgtact
     3781 ctgttaacca gcgtctttat cggagaataa ttgcctttaa tttttttatc tgcatctcta
     3841 attaattatc gaaagagata aatagttaag agaaggcaaa atgaatatta tcagttctgc
     3901 tcgcaaacga attcccgtac actgaactct ctggctcact gagggcacca tcatggcact
     3961 cccccgtatt acccaaaaag agatgactga acgcgagcag cgtgaactta aaacactgct
     4021 ggatcgggcg cggattgcgc atggtcgcgt actgaccaac tccgaaacca acagcattaa
     4081 gaaagagtac atcgataaat taatggtcga gcgtgaggct gaagcgaaaa aagcccgcca
     4141 gttaaagaaa aagcaggctt ataaaccgga tcctgaagcg tcatttagct ggtcagcaaa
     4201 tacgtcgacg cgcggcaggc gctgattagc gccccttctt ttttcgcccc ggcgaggtaa
     4261 aacgcttacc gttggacgcc gggcgaccgc ctttttccgc cgttttttcc atcttaacga
     4321 ctggacgttt gatgcccgct gttttcggtt tggccttcgc tttaggtttt gcctcggaag
     4381 aggaattttc gatgagctta aagaggtcga ttaactcatc gtcggttaaa tcgcgccact
     4441 cccccagcgg aatgccgctt aagctgacgt tcataatgcg cgtacgttcc agctttttca
     4501 cttcatagcc gaaatgctcg cacatgcgcc ggatctgacg gttcagcccc tgcaccaggg
     4561 taatgcggaa gacaaacggc gcttcttttt tgactttgca ctttttagtc accgtcccga
     4621 ggatcggcac ccccgccccc ataccacgaa taaattcatc ggtaatcggt ttatcgaccg
     4681 tcaccagata ctctttctca tgatcgttac ccgcacggag gatcttattc accagatcgc
     4741 cgtgattggt gaggaaaatc agcccctggg agtctttatc caggcggcca atcgggaaca
     4801 cgcgtttgct gtggttaacg aaatcgacaa tgttatcgcg ctcgccatct tcggtggtgc
     4861 tgacaatgcc tacgggcttg ttcagggcga taagtaccaa atcttcggct tcccgaggct
     4921 caatcaactg accgtttact ttcacaacgt cgccgggttt cacctgatcg ccaatggtgg
     4981 ctcgcttgcc attaaggaac acattgcctt gctcgatata gcgatccgct tcgcggcgtg
     5041 agcaaattcc gctttcgctg atgtatttat ttaaacggac tgatgagtcg ggcagcataa
     5101 attctcctgt aaaacgcgga atataccgta cctcttagcg gggaaaaaca gggttatgcg
     5161 gcaacgtcga aattaaatgt gatctgccac gccttttatc acaaaagtgt ctccggaagt
     5221 acaatgcaaa tttgcgtggc aaaataagga taatcaacat attacgaatc gctgcacacc
     5281 tgtgctgata actactgcgc agagaaacaa atgtgcaaga gaaagcgcta tttctcccca
     5341 tgcaaagagt gaaaaatcgc tttgaaaggc catcatgatg gggttatatc agatcaagcg
     5401 gtgagaaagc gtatttgcac aaatgtgcag aaccaggagg cggcaatgga aaacagtgac
     5461 gatatccgtt tgattgtgaa gattgcccaa ctctattacg aacaggatat gacgcaggcg
     5521 caaatcgcgc gcgaactggg tatttaccgc accaacatca gccgcttgct taaacgaggc
     5581 cgcgatcagg gaattgtcac catcgccatc aactatgact acaacgaaaa tctctggctg
     5641 gagcagcaac tgaagcaaaa gtttggcctg aaagacgttg tggtggtgtc gggaaatgat
     5701 gaggatgaag agactcaact ggcgatgatg gggttacacg gcgcgcaact gctggatcgc
     5761 ttgctggagc ctggcgatat tgtcggtttt tcctggggtc gcgcggtgag cgcactggtt
     5821 gaaaacttgc cgcaggcggg gcaatcgcgg cagttaatct gcgtaccaat tattggcgga
     5881 ccgtccggta aactcgaaag ccgctatcac gtaaacacat taacctacag cgcggcagcg
     5941 aagctgaaag gggaatcgca tctcgcggat tttccggctc tactggataa cccattaatt
     6001 cgtaatggga tcatgcagtc tcagcacttt aaaaccatct ctgcctactg ggataatctg
     6061 gatgtcgccc tggtgggaat tggctcaccg gccattcgcg acggcgctaa ctggcatgcg
     6121 ttttatggtg gtgaagagag tgacgacctg aatgcccgcc aggttgctgg cgatatttgc
     6181 tcgcgctttt ttgatattca cggcgaaatg gttgaaacga atatgagcga aaaaacactc
     6241 tctatcgaaa tgaataaatt aaagcaggca cgatattcca ttggcattgc catgagtgaa
     6301 gaaaaataca gcggaattat tggtgcactg cgtggaaaat atattaattg tctggtaacg
     6361 aatagcagca cagctgaact attactgaaa taaaaatctt cccgatgttt atcgtaataa
     6421 aaattacggc gggtccctat tatctaaaat caggagtctg ttatgcaaac gtggttaaat
     6481 ttgcaggata aaatcattat cgtcaccggc ggcgcatccg gtattggtct ggcgattgta
     6541 gaggaattat tagcacaagg cgcaaacgta cagatggtag atattcacgg tggcgatgga
     6601 caatatgaag gtcataaagg ctatcagttt tggccgaccg atatttccag cgccaaagag
     6661 gtaaatcata cggtagcgga aattatccag cgttttggtc gcatcgacgg tctggtcaat
     6721 aacgccgggg tcaatttccc gcgtctgctg gtcgatgaga aagcgcctgc cgggcagtat
     6781 gaactcaacg aagctgcatt cgaaaaaatg gtcaatatca accagaaagg cgtttttctg
     6841 atgtcgcagg cggtggcgcg acagatggtc aaacaacatg atggcgtgat tgtgaatgtt
     6901 tcctcagaaa gtgggctgga aggctcagaa ggccaaagct gttacgccgc taccaaagcc
     6961 gcgctcaata gcttcacgcg ctcctggtcg aaagagctgg gtaaacacgg tatccgtgtg
     7021 gtcggtatcg cgccggggat tctggaaaaa accgggctac gcacgccgga atatgaagag
     7081 gcgctggcgt ggacgcgcaa tatcaccgtc gagcagctgc gtgaaggcta taccaaaaac
     7141 gccattccta ttgggcgcgc cggaagatta gcagaaatcg ctgattttgt ttgttatctg
     7201 ctgtctgaac gcgccagcta tatcaccgga gtaaccacta acattgcggg cggcaaaacg
     7261 cgcggctaag gaggctttat ggtcaacgcc attttttgtg cccacggcaa attagcttgc
     7321 gccatgctgg aatcggttca gatggtttac ggcaacgcca atgttgaagc cgttgaattt
     7381 gtgcccggtg aaaacgccgg agacatcgta gcaaagctgg aaaaattagt aagcattcac
     7441 aatcaaaatg agtggctgat tgccgtggat ttacagtgcg gcagcccgtg gaatgcggca
     7501 gcgatgcttg cgatgcgcaa tcctcgtctg cgggtaatca gcggactttc tctgccgctg
     7561 gcgctcgaac tggttgataa ccaggacagc atgaatgttg atgaactgtg tgaacacctg
     7621 acgcaaatcg caaagcaaac ctgcgttgtc tggaaacaac tggcaacgac agaggaggat
     7681 ttctgatgaa cattacgctc gcccgcattg atgaccgcct gatccacggt caggtcacta
     7741 ccgtctggtc gaaagtggct aacgcccagc gcattattat ctgcaatgac gaagtttata
     7801 acgatgaagt tcgccggaca ttattgcgtc aggctgcgcc accgggaatg aaggttaacg
     7861 tggtcaatat tgaaaaagcc gtcgcggttt atcacaaccc gcaatatcag gacgaaaccg
     7921 ttttttatct tttcactcgc ccacaggatg ccttagcgat ggttcgccag ggggtaaaaa
     7981 taggcaccct gaatattggc ggcatggcct ggcggcccgg taaaaaacag ttaaccaaag
     8041 cggtttcatt agatgatgat gatattaacg catttcatga gttaaataat ctaggcgtca
     8101 tactggattt acgcgtcgta gcgtcagatc cctcaatcaa tattatctac aaaataaacg
     8161 aacagttaat cgcaaattaa ataatagcgc ctgtgtacat atgctttaac aggcagggat
     8221 gacgacactt aaaaggtgac aaatcatgga aataagtacc ctacaaatca ttgctatatt
     8281 tcttttttcc tgtattgccg gaatgggcag cgtgctggat gaatttcaga ctcatcgccc
     8341 gttaattgcc tgtacggtga ttggtttaat tctcggtgat ttaaaaaccg gaataatact
     8401 cggtggtacg ctggaattga tagctctcgg ctggatgaac gtcggcgcgg cgcaatctcc
     8461 ggattctgca ctcgccagca taatctccgc cattctggtt atcgttggtc agcaaagcat
     8521 cgccaccgga atcgccatcg cgttgcctgt ggctgcggca ggccaggtgc tgacggtgtt
     8581 tgcccgtacc atcaccgtag tgttccagca cgcggcggat aaagcagcgg aagaagcacg
     8641 gtttcgcact ctcgatattc tgcatgtctc cgcgcttggc gtgcaggcgc tgcgcgtcgc
     8701 cattccggca ctgattgtct cgctgttcgt cagcgccgat atggtgagca atatgttaaa
     8761 cgccattccc gaatttgtga cccgaggtct gcaaattgct ggcggtttta tcgtggtagt
     8821 cggttacgcc atggtgctac gtatgatggg cgtgaaatat ttgatgcctt tctttttcct
     8881 cggtttcctc gcaggtggct acctcgatct cagtctgctg gcgttcggtg gcgtcggcgt
     8941 gatcatggcc ctggtctaca tccagctaaa tccacagtgg cgtaaagctg aacctcaact
     9001 cctggccccc gcttccaccg cccttgacca acttgacgat taacggagcc atcatggaac
     9061 agagaaaaat tacactcggc gatctggtga gcatgtttct gcgctccaac ctgcaacagg
     9121 cgtcctttaa ctttgaacgt attcacgggc tgggtttttg ctacgacatg atccccgcca
     9181 tcaagcgact ttacccagta aaagaggatc aggttgcggc gctcaggcga cacctggtgt
     9241 tctttaatac cacgccagcc gtatgtggcc cggtcatcgg cgtcactgcc gccatggaag
     9301 aggcgcgggc caacggcgcg gaaattgatg acggcaccat taacggcatc aaagtcggtc
     9361 tgatgggacc attggcaggg gttggcgatc cactggtctg gggaacgctg cgcccgatta
     9421 ccgccgcgct cggcgcatcg ctggcgctgt ctggcaacat tctcggcccg ctgctgttct
     9481 tctttatttt caacgcggtg cgtctggcga tgaagtggta tggcctacag ctcggctttc
     9541 gcaaaggggt gaatatcgtc agcgatatgg gcgggaatgt gctgcaaaaa ctcaccgaag
     9601 gcgcgtcgat tctcggactg tttgtgatgg gcgtgctggt caccaaatgg acgtcaatca
     9661 acgtaccgtt ggtggtttca caaacgcatg ccgccgatgg ctccaccgtc accatgaccg
     9721 tgcagaacat tctcgatcaa ctttgccctg gtttgctggc gctcggtctg acgctactga
     9781 tggttcgtct gctcaacaaa aaaattaacc cggtatggct gattttcgcc ctgtttggct
     9841 tagggattat cggcaatgcg ctgggcttcc tgtcctgatt cttcgccccg gcacgactgc
     9901 cggggccatc gttcaacatg aggtggttta tgaaaacaac agctctgcgt ctttatggta
     9961 aacgtgattt acgcctggaa acctttgacc ttcctgaaat gcaggaggat gaaatcctcg
    10021 cgacggtggt cactgacagc ctgtgcctct cttcctggaa agaggccaat ctgggtgaaa
    10081 accataaaaa agtacccgac gatgtggcga ccaacccaat catcatcggc cacgagtttt
    10141 gcggcgatat tctggccgtg ggtaaaaagt ggcagcacaa attccagccg ggtcagcgtt
    10201 atgtgattca ggccaacctg caactccccg accgcccgga ctgccccggc tactccttcc
    10261 cgtgggtagg cggcgaggcc acgcatgtgg ttattcccaa cgaggtcatg gaacaagatt
    10321 gcctgctggc atacgacggc gaaacctatt ttgaaggctc gctggttgaa ccgctttcct
    10381 gcgtgattgg cgcgttcaac gccaactatc atcttcagga aggtagttat aaccacacga
    10441 tggggattcg cccgcaaggg cgcatgctga tcctcggcgg caccggacca atgggactgt
    10501 tggcgattga ttatgcgcta catggacccg ttaacccgtc gctgctcgtc attaccgata
    10561 ccgacaacga taaattgagt tatgcgcgca agcactatcc atcagaaccg caaacactga
    10621 ttcattatct caatgccgcc gatgcagcat ttgatacgct aatggcgctg agtggcggtc
    10681 acggcttcga tgatattttc gtctttgtgc ctaatgaagg actggtgact ctcgcctctt
    10741 ccttgctggc gacagatggt tgcctgaatt tcttcgccgg accgcaggat aaacatttca
    10801 gcgcgccaat taatttctac gatgtgcatt atgcatttac ccactacgtg ggcacgtcag
    10861 gcggcaatac cgacgacatg cgcgcagcgg tcaaattgat tgaagagaaa aaagtgcagg
    10921 ccgcaaaagt ggtaacacat attcttgggc tgaatgccgc gggcgaaacc acgcttgaat
    10981 tgcctgccgt cggcggcggc aaaaagctgg tgtataccgg gaaatacctg ccgctgacgt
    11041 cactcacgca gattcaggat caagcactgg cggcgattct ggcgcgtcat caggggatct
    11101 ggtccggtga ggcggagcaa tatctgctca ctcatgcaga ggcaatttcc catgattaat
    11161 cgcgctactt aactctgcat gtcgctggct ggtcaccgcg gcaacttcgc gacctgatgt
    11221 gcggctgatt gctgaagcgg cccaatgtgc acgagaagga taaaaaaaca tcagcgcctt
    11281 aaggggcgct caaattggtg gcaaagtaca cattgtttat gccggatgcg gaataaagcc
    11341 ttattcggcc tacataatct cgaaaattca atatattgca gttatctggt aggcctggta
    11401 agcgtagcgc atcaggcaat gctgtgtttg tcatcagtct cagcgcccgg tgcaatacgt
    11461 gacatcgtga gccatgtcag taagtggtta aattcgcagt ggtagcatgc cttccgtgat
    11521 ttcttactaa ccggagaagt aatggaactg cttttattga gtaactcgac gctgccgggt
    11581 aaagcctggc tggaacatgc actgccgctg attgctgaac agttgcaggg tcgccgctca
    11641 gcggtgttta tccctttcgc tggcgtaacg cagacctggg atgattacac agcgaaaacg
    11701 gctgcggttc tcgctccgct gggtgtttct gtcaccggta ttcatagcgt tgtcgatccc
    11761 gttgccgcga ttgaaaatgc ggaaattgtg attgtcggcg gcggtaatac tttccagttg
    11821 ctgaaacagt gccgcgagcg cgggctgctg gctccaatta ctgacgtggt taaacgtggc
    11881 gcgctgtata ttggctggag cgcaggcgct aaccttgctt gcccaaccat tcgtaccacc
    11941 aacgatatgc cgattgtcga tccgcaaggt ttcgatgcgc taaatctgtt cccgttgcaa
    12001 atcaacccgc acttcaccaa cgcgctgccg gaaggccata aaggtgaaac ccgtgagcag
    12061 cgtattcgcg aactgctggt cgtcgcgcca gaactgacga ttattggtct accggaaggt
    12121 aactggatca cagtgagtaa aggtcacgca acgctgggtg gcccgaatac cacctatgtg
    12181 tttaaggccg gtgaagaagc ggttccgctg gaagctggtc accgttttta agtgaattgc
    12241 cggatgcatg atccggcatc ttgttcatag tgcctgatgc gacgcttgac gcgtcttatc
    12301 aggcctacag gattcacaat attaatactc gtcccgtccc tcatcttcat caggctgttc
    12361 cagcacactg taagccaccg aacaaaacag cgagttcaga cgctgcatat ctcccagtaa
    12421 gcctaaatgt agcgaactgg tttcaatgct ttgcacgttt tgctgatgca ggcgatcgac
    12481 gtgggcgtgg gaatagcggc gattaagaat gcgaaagcga tgcttgctgc ggcgcaaacg
    12541 acgagcgctg gtgacatcgc cagagaagaa caccgacatt gccagcttta aattactgag
    12601 caattgctca tagagcgcat ccagttcctt caagccatca agcgaaaatg ctcgccttgc
    12661 agccagcgat ttgtcggcaa tttcactact catgcgctcg acgatatcgg aggcctgttc
    12721 aaggttgagc gacatttcga tgatctccgc ccagcggcgc gactcttctt ctgccagttc
    12781 ctcttttggc atccgcgcca gatacagctt aatggcggta tagagaacgt tgatatcatc
    12841 tgccagctta cgcagctctt tctcctgcct tggctcgccg tgcatcactt tattcagccc
    12901 ttccatcatc tgttccatgg cgtcgccaat gcgcagggtt tcgcgcgcgg cgttcgccag
    12961 agcaagcgtg ggcgtatcca gcgcgctgac atccagatgt ttaggccgta gctgggtatc
    13021 cagttccggt tcatcgcgaa taatcgtttt gcaaaaccgt gccatcgggt caacaaatgg
    13081 cagcatggcc aggcaacgta caaggttgta gaagacgtgg aaatagatca ccagttccgc
    13141 ttttggcaat gacaacttcc ccattgtctc tgccagcaaa tggacaaacg gcaggataat
    13201 caggctaccc accagcttaa acagcagact acccagcgcg acacggcggg ctgcggcatt
    13261 ggcggcactg ttgttgagca tcgccagcag gccggaaccg aggttagcac caatcaccag
    13321 acagagcgcc acggggaagg agataatgcc tgcggcggtc agagtcgcgg tcagcagtac
    13381 agcagcaagg ctggagtagc tgataatggc gaacatcgcg ccaatcagcg catccagcag
    13441 aatatcgccg gtcagcgagg caaagatcac ctgaacgccg tttgcctggg tgatcggcgt
    13501 tacggcctgc acaatcaact ccagcgccag caaaatcagc ccaagaccaa taccgacgcg
    13561 gcccagttgc ccggcgcgtg actgtttgcg tccgaggaaa aaaatcacgc cgataaaaat
    13621 aagtaacggt gacagccagg ataagtcgaa ggtgagaata cgcgccatta gcgccgtccc
    13681 gacatctgcc cccagcacaa tgaccagagc cggtgcgagg gctaccagat cctgagcgac
    13741 gaacgaggtc accagcatgg tggtggcatt actgctctgt accagtgcgg taacgccgat
    13801 ccccgcgcaa aaggcgagcg gctttttttc gacgctccgg ctaaggacag tacgcaaacg
    13861 cgcgccgaag acgcgcatta cgccggttcg aacaatatga gtcccccaga ccagcagggc
    13921 gacggcagaa agcaggttaa gcagcgttaa cacgagttca ggttctcctt atcgttatat
    13981 gttcctgaag caattgaatg accaaaggac cggaaacacg ctgactggca tctttactgt
    14041 gccttttgac attcgttttt ccggctaaga ttattccagc ctgtttccag tataagtgtt
    14101 tcatattaat aaagagacag ggcactcatt gagcgccctg tttgttgtaa ggaaaagtga
    14161 cagatttgtg aatcagtccg cgtcataccc cagattcggt gccagccagc gctcaacatc
    14221 ggagacgctc atacctttac ggcgggcata gtcttcaacc tgatcgcgct gaatttgcgc
    14281 caccgcgtag tacttgctgt ccgggtggct gaaataccaa cccgaaaccg atgcaccggg
    14341 ccacatggcg aaagattctg tgagtttcat gccggtatgt ttttccactt ccagcagctc
    14401 ccagatggtg gctttttccg tatgttccgg gcaggccggg tagcccggtg ccggacggat
    14461 gccctggtag ttttcgcgga tcagttcttc gttgctgagg ttctcgttcg gcgcatagcc
    14521 ccagtagact ttacgcacac gctcatggag atactccgca aaggcttcgg ctaaacggtc
    14581 ggcaagcgct ttcaccatga ttttgttgta atcatcgtgc tgcgcttcaa aggcatcagc
    14641 cagtgcgtcc tcttccagcc cgccagtcac ggcaaatgcg ccgatgtaat ctgctttacc
    14701 agaaagcttc ggcgcaacga agtcagcgag acagtagtta gcgaatcccg ttttttcggt
    14761 ctgttgacgc agatggtggc tgacgttgat cacatgggtg cgcgtttcgt cacggtagat
    14821 ttcaatgtca tcgcccacac ggtttgccgg gaacaggccc accacgccac gcggattcag
    14881 cgttttctcg gcgcttaatt tatccagcat gtcgttggcg tctttaaaca gccgctgcgc
    14941 ctcaacgccc accacttcat cttccagaat gcgcggatac ttcccggcca gcgaccaggt
    15001 cataaagaac ggtgtccagt cgatgtaatt acgcagagtt tcgatgctgg cttcaacttc
    15061 ctgcacgccg agacggtgcg ccaccggcgg cgtgtaagcc tgccagtcaa aagcgaagtc
    15121 gttatcgcgc gccgcttcca gcgtgaccgg tggtgtgcgt ggtttcttgc gcccgtgctg
    15181 aatacgtacg gtttcgtact ccttgcgggt acgagcgaca aaatcatcgc gctgagtatc
    15241 ggaaagcagc gccgccacca caccaacggt gcgcgaggca ttctgcacat acaccgtcgg
    15301 gccgctgtag ttctgctcga ttttcaccgc cgtgtgcgct tttgaggtcg tcgcgccacc
    15361 gatcagtaac ggaatggtga agccctgacg ctccatctct ttcgccacgt taaccatctc
    15421 gtccagcgac ggcgtgataa gccccgaaag tccaatcaga tcagcattca cttctttagc
    15481 ggtacggaga attttttccg caggcaccat aacgccgaga tcgacaattt cgtagttgtt
    15541 acattgcagc accacaccaa cgatattttt accgatgtcg tggacgtcgc ccttcacggt
    15601 ggcgatcacc atcttgccgt tggttttgcc ctgctctttg ctggcttcaa taaacggttc
    15661 gaggtaggcc actgcctgct tcatcacacg cgccgatttg accacctgtg gcaggaacat
    15721 tttcccttcg ccgaacagat cgccgaccac gttcatgccg tccattagcg ggccttcaat
    15781 cacttcaatc gggcgcgtag cctgctggcg ggcttcttcg gtatcctgct cgataaactc
    15841 agtaatgcct ttgaccagcg agtattccag acgtttattc acttcccacg agcgccactc
    15901 cgcctgctgg gcgttagcga tgtcgtcggt tttgctgccg cgatatttct cggcaagctc
    15961 cagtaaacgc tcagtgccat catcgcgacg attaagaatc acatcttcca ccgcgtcgcg
    16021 cagttcagcg ggcaggtcgt cgtaaatcgc cagttgcccg gcgttgacga tccccatatc
    16081 catgccattg cgaatagcgt agtagaggaa caccgcgtga atggcttcgc gcaccggatc
    16141 gttgccacgg aatgagaaag aaacgttaga tacgccgccg gaaatcagcg cgtgcggcag
    16201 ttcgcgtttg atgtcttcgc aggcgccgat aaagtcctgc gcatagttgt tgtgctcttc
    16261 aatgccggtg gcgaccgcga agatgttcgg gtcgaagatg atatcttctg gcgggaagcc
    16321 aacctcttcg gtgaggattt tgtacgcccg acggcaaatc tcaattttcc gtgcgcgggt
    16381 atcggcctgc ccctgttcgt caaaggccat taccaccacc gctgcaccgt aacgacgcaa
    16441 cagtttcgca tgatggataa aggcgtcgac gccctctttc attgagatag agttaacaat
    16501 gcctttgccc tgaatacact tcagcccctt ttcgatgacc tcccattttg aggagtcgat
    16561 cataatcggc acgcgagcga tatccggttc accggcaatc agattgagaa aacgcaccat
    16621 cgccgcttcg gcatcgagca tcccttcatc catgttgata tcgataatct gcgcgccgtt
    16681 ttccacctgc tgacgcgcta catccagcgc ctcgctgtat ttctcttctt tgatcaggcg
    16741 cttgaactta gcggaaccgg tgacgttggt gcgttcaccc acgttcacaa acaggctatc
    16801 gtcgccaatg ttcagcggct ccaggccgga caaacggcag gctacgggaa tttcaggcag
    16861 tttgcgcggc gctaatcctt ctactgcacg actcatcgct gcaatatgtt gtggcgtggt
    16921 gccacagcag ccgccgacga tattgagaaa acccgcttgc gcccattcac gtatctgttt
    16981 tgccatcgtg tcggcgtcga gatcgtactc accaaaggcg ttgggtagcc cggcgttcgg
    17041 gtgcgcggtg acgtagcatt ccgcaatccg tgatagttcc tgcacgtact ggcgcagttc
    17101 atctggcccc agcgcgcagt tgaggccaaa agtcagggct tcggcatggc gcagggagtt
    17161 gtaaaatgct tcggtggtct gtccggagag cgtgcgcccg gaggcgtcgg tgatggtgcc
    17221 ggagatcata atcggcagct caacgcctaa cgcttcgaac tccgttttca ccgcaaagac
    17281 cgccgctttg gcgttaaggg tgtcgaaaac ggtttcaatc agaatcagat ccgcaccgcc
    17341 ttccactaat gctttggtgg attcgcgata ggccgccacc agctggtcaa aagtgatgtt
    17401 gcggaatgcc ggatcgttca cgtccggaga aatggacgcc gtgcggttgg ttgggccgag
    17461 aacgccggca acgtagcgcg gtttctccgg cgtgcgcgcg gtccactcgt cagcacaggc
    17521 gcgcgccagt ttcgccgccg caaagttgat ttccgccgac agggattcca tctggtaatc
    17581 cgccatcgca atggtcgtgg agttgaaggt gttggtttcg atgatatccg cgcccgcttc
    17641 aaagtaggcg ttgtggatag cggcgatcac ttccggtttg cttagcacca gcagatcgtt
    17701 attgcctttg aggtcgcatg gccagtcggc aaagcgttca ccacgaaaat cggcttcgtt
    17761 cagtcgatag ctctggatca tggtgcccat accgccgtcc agcaccagaa tacgttcatt
    17821 taactgcgca cgcagttgtt ccactttgct gctcacactt gctcccgaca cgctcaaccc
    17881 agatttaata aaaattcaac aaaccatact ggcataaacg catctgtggt aaaagcgacc
    17941 accacgcaac atgagatttg ttcaacatta actcatcgga tcagttcagc aactattgca
    18001 ttagctaaca ataaaaatga aaatgatttc cacgatacag aaaaaggaga ctgtcatggt
    18061 cgcacccatt cccgcgaaac gcggcagaaa acccgccgtt gccaccgcac cagcgactgg
    18121 acaggttcag tctttaacgc gtggcctgaa attactggag tggattgccg aatccaatgg
    18181 cagtgtggca ctcacggagc tggcgcaaca agccgggtta cccaattcca cgacccaccg
    18241 cctgctaacc acgatgcaac agcagggttt cgtgcgtcag gttggcgaac tgggacactg
    18301 ggcaatcggc gcacatgcct ttatggtcgg cagcagcttt ctccagagcc gtaatttgtt
    18361 agcaattgtt caccctatcc tgcgcaatct aatggaagag tctggcgaaa cggtcaatat
    18421 ggcggtgctt gatcaaagcg atcacgaagc gattattatc gaccaggtac agtgtacgca
    18481 tctgatgcga atgtccgcgc ctatcggcgg taaattgccg atgcacgctt ccggtgcggg
    18541 taaagccttt ttagcccaac tgagcgaaga acaggtgacg aagctgctgc accgcaaagg
    18601 gttacatgcc tatacccacg caacgctggt gtctcctgtg catttaaaag aagatctcgc
    18661 ccaaacgcgc aaacggggtt attcatttga cgatgaggaa catgcgctgg ggctacgttg
    18721 ccttgcagcg tgtattttcg atgagcaccg tgaaccgttt gccgcaattt ctatttccgg
    18781 accgatttca cgtattaccg atgaccgcgt gaccgagttt ggcgcgatgg tgattaaagc
    18841 ggcgaaggaa gtgacgctgg cgtacggtgg aatgcgctga ctttttctgg cgggcagagg
    18901 caatattctg cccgtcatac ctgagtggta atagaataag ggtgtctgtt aatcgcattg
    18961 acgtcaaaat aacttattgt catacacttc atacactttt attaactcag cattattttt
    19021 aaacatcaaa ccacttaatt attaacaaaa tcgcaagatc tcattccacc gaaactttta
    19081 c
//
LOCUS       DAZKIP010000081        19008 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZKIP010000081 DAZKIP010000000
VERSION     DAZKIP010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19008
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.081"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(66..431)
                     /gene="yhaH"
                     /locus_tag="ACHOUK_003146"
     CDS             complement(66..431)
                     /gene="yhaH"
                     /locus_tag="ACHOUK_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFP9898539.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            complement(723..1709)
                     /gene="yqjG"
                     /locus_tag="ACHOUK_003147"
     CDS             complement(723..1709)
                     /gene="yqjG"
                     /locus_tag="ACHOUK_003147"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFP9898540.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVEKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTRIISIGPWQDLDEPHGRDVRLG"
     gene            complement(1779..2171)
                     /gene="yqjF"
                     /locus_tag="ACHOUK_003148"
     CDS             complement(1779..2171)
                     /gene="yqjF"
                     /locus_tag="ACHOUK_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFP9898541.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITGYAGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            complement(2357..2656)
                     /gene="yqjK"
                     /locus_tag="ACHOUK_003149"
     CDS             complement(2357..2656)
                     /gene="yqjK"
                     /locus_tag="ACHOUK_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFP9898542.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            complement(2646..3050)
                     /gene="yqjE"
                     /locus_tag="ACHOUK_003150"
     CDS             complement(2646..3050)
                     /gene="yqjE"
                     /locus_tag="ACHOUK_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFP9898543.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            complement(3053..3358)
                     /gene="yqjD"
                     /locus_tag="ACHOUK_003151"
     CDS             complement(3053..3358)
                     /gene="yqjD"
                     /locus_tag="ACHOUK_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFP9898544.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            complement(3396..3764)
                     /gene="yqjC"
                     /locus_tag="ACHOUK_003152"
     CDS             complement(3396..3764)
                     /gene="yqjC"
                     /locus_tag="ACHOUK_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFP9898545.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            complement(3911..4294)
                     /gene="mzrA"
                     /locus_tag="ACHOUK_003153"
     CDS             complement(3911..4294)
                     /gene="mzrA"
                     /locus_tag="ACHOUK_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFP9898546.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPQGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            complement(4298..4960)
                     /gene="yqjA"
                     /locus_tag="ACHOUK_003154"
     CDS             complement(4298..4960)
                     /gene="yqjA"
                     /locus_tag="ACHOUK_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFP9898547.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            complement(4982..5065)
                     /gene="yqiM"
                     /locus_tag="ACHOUK_003155"
     CDS             complement(4982..5065)
                     /gene="yqiM"
                     /locus_tag="ACHOUK_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFP9898548.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            complement(5305..6081)
                     /gene="exuR"
                     /locus_tag="ACHOUK_003156"
     CDS             complement(5305..6081)
                     /gene="exuR"
                     /locus_tag="ACHOUK_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFP9898549.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            complement(6211..7509)
                     /gene="exuT"
                     /locus_tag="ACHOUK_003157"
     CDS             complement(6211..7509)
                     /gene="exuT"
                     /locus_tag="ACHOUK_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFP9898550.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            7992..9404
                     /gene="uxaC"
                     /locus_tag="ACHOUK_003158"
     CDS             7992..9404
                     /gene="uxaC"
                     /locus_tag="ACHOUK_003158"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFP9898551.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMSGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            9419..10906
                     /gene="uxaA"
                     /locus_tag="ACHOUK_003159"
     CDS             9419..10906
                     /gene="uxaA"
                     /locus_tag="ACHOUK_003159"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFP9898552.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVRLRQDVARGHKF
                     ALTNIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            10989..11540
                     /gene="ygjV"
                     /locus_tag="ACHOUK_003160"
     CDS             10989..11540
                     /gene="ygjV"
                     /locus_tag="ACHOUK_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFP9898553.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(11545..12789)
                     /gene="sstT"
                     /locus_tag="ACHOUK_003161"
     CDS             complement(11545..12789)
                     /gene="sstT"
                     /locus_tag="ACHOUK_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFP9898554.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(13188..14153)
                     /gene="alx"
                     /locus_tag="ACHOUK_003162"
     CDS             complement(13188..14153)
                     /gene="alx"
                     /locus_tag="ACHOUK_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFP9898555.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRVG"
     gene            complement(14437..15423)
                     /gene="ygjR"
                     /locus_tag="ACHOUK_003163"
     CDS             complement(14437..15423)
                     /gene="ygjR"
                     /locus_tag="ACHOUK_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9898556.1"
                     /translation="MIRFAVIGTNWITHQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     EFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(15502..16194)
                     /gene="ygjQ"
                     /locus_tag="ACHOUK_003164"
     CDS             complement(15502..16194)
                     /gene="ygjQ"
                     /locus_tag="ACHOUK_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFP9898557.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWSDVNVVPARNVGLLLGARPGNRYFTRRIDTAAELYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRANKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLEKGRGKIVRLREKLARVSAVIDAKIFNRQPKYLGPSVII
                     GPFSEHGCPAQK"
     gene            complement(16271..16774)
                     /gene="ygjP"
                     /locus_tag="ACHOUK_003165"
     CDS             complement(16271..16774)
                     /gene="ygjP"
                     /locus_tag="ACHOUK_003165"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFP9898558.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            16859..17995
                     /gene="rlmG"
                     /locus_tag="ACHOUK_003166"
     CDS             16859..17995
                     /gene="rlmG"
                     /locus_tag="ACHOUK_003166"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFP9898559.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTPQTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            18279..18593
                     /gene="higB"
                     /locus_tag="ACHOUK_003167"
     CDS             18279..18593
                     /gene="higB"
                     /locus_tag="ACHOUK_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HFP9898560.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            18590..19006
                     /gene="higA"
                     /locus_tag="ACHOUK_003168"
     CDS             18590..19006
                     /gene="higA"
                     /locus_tag="ACHOUK_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFP9898561.1"
                     /translation="MIAIADILHAGEKLTAVAPFLAGIQNEEQYIQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
BASE COUNT         4866 a         4918 c         4817 g         4407 t
ORIGIN      
        1 attattcatt catcaacatg atgataaaag ccggatgagt cagacatccg gcgagcagat
       61 ttgttttaag gttccagttt tggatcaggt ccaaagcggt tttcgcctgg cgtacccgcc
      121 tggcagttaa agacgatgat aataagccag ccgataaacg ggattaagaa tagcagtgcc
      181 caccacgccg agcggtcggt gtcgtgcagg cggcggaact gaactgccca ccacggtaaa
      241 aacaccagaa taccgtagat tgtcgtcagg atgccttcgc caccagcgcg ttgccagcct
      301 aacattttat ccagcagccc cagcacgaac gtaaagatga tgttgaccag aataaacatc
      361 cagtactctt tgcgccgcgc acgaccccgg aaaccgacat aattttttag tactttcaga
      421 taccagtcca tttttgcctc aattttgctc aatcacttgt ttttaaagca gcaaattaag
      481 aatagacgtg aatgaaatgg cagtaaatat tcgttcaagg aattaataaa gtgctgtgaa
      541 ggcgcgctaa gactgatgac aagcgcaaaa ttgccagatg cgctacgctt atctggcccg
      601 cgcggtcatt gcaacatatt gaatataaaa gttttgtagg tttgataagg cgttcatgcc
      661 gcatccgaca tgaacaacat gcacttagct aattatcaga ggcacctcgt aaggtgcttt
      721 ttttaaccta agcgaacatc tcgtccatgc ggttcatcga gatcctgcca cggaccaatt
      781 gaaataatcc gcgtagggtt gatggtctta tgactgcgga agtaatgatt acggatatga
      841 tcgaaattga ctgtttcggc aattcccggc atctggtaga tatcacgcag gaagccatac
      901 agattaaggt aatcgctgat gcggtgctta tcacacttga agtgggtcac atacactgga
      961 tcaaaacgga ccagcgtggt ccacaggcga atatcggctt cggttagctg gttgccggtc
     1021 aggtaacgat gctgacctaa aatctgttcc agtcgcgcca gcgattcaaa cactttctcc
     1081 accgcttcgt cgtaggcttg ctggctggtg gcaaaaccag ctttatacac gccgttgtta
     1141 acggtgtcat aaatccagcc gttaagttcg tcaattttcg tttgcagggc tggtgggtag
     1201 taatcacccg ctttcgcgcc cagcgcatca aacgcggtat taaacatgcg gatgatttct
     1261 gctgattcgt tgctgacgat ggtgtggttc tttttgtccc acagcacggg aacagtaact
     1321 cgtccgctgt agtgtggatc ggcgtggaga taaagctgat acaaaaattc atgttgatag
     1381 agcgtgtctc cggttgctcc cggaaaactg tcatcaaagg tccagccgtt ttccagcatc
     1441 agcgggttca ctacggaaac ggaaataaac ggttccagtc ctttgagttt gcgcatgatc
     1501 agcgtgcggt gcgcccacgg gcaggcgagt gaaacataga gatgataacg atctttctct
     1561 gcgataaaac cgcctgtgcc agtggggcca ggagcgccat cggcagtgag ccagttacga
     1621 aatgcggaag ctgaacgttg aaatttaccg ccggtagatt tggtatcgta ccaggtgtca
     1681 tgccagacgc cgtcaatcag ttgacccatt gttctctcct ccaatagcaa aaacgaggag
     1741 cttcctcctc gttttttatc gttaagtata gcgcgtgctt accacttttt attcagcagg
     1801 cggtcgatgc tatacgcgcc cggaccggta attgccagca gcaggaatcc gccagaaatt
     1861 gtcaggtttt tcatgaacat cagcgagttg acgccttcag caaagttgct gtgaaataaa
     1921 aatgccgtca gcagcgtaaa gcccgcagta aacagggctg tggtgcgagt caggaaaccg
     1981 aacaggattg ccagaccacc accaaactca agcagaatca ccagcggcag cataaaaccc
     2041 gggacgccca tcgcttccat atattgttgg gtacccgcgt aaccagtaat ttttccccag
     2101 cctgcggtaa taaacagaat cggcattaaa atgcgcgcta ccagtacacc aacatcttct
     2161 aattttttca tcatactctc cactgcgcca ctcaggctgc tgattgtttt tttgtgcaag
     2221 ggcgcggtat tagcgtcgtt gctgtcgatg gagagaatca taaacgtggt gaatgatgat
     2281 tgttagcaag gaaaactgtc aaaaatcttc aaaaaatttg atgggtaagg ccggaatggc
     2341 tccggccaga gggaagttaa ccgcgaagct gttgctgctt gagggtcgtt ttaaccagac
     2401 gccaggcgct ccatacgcca aaaccgcgtc tggcccagcg gaccagcata ttaggatggc
     2461 gaatcgtcca gatcgccatc acgctactgc caaccagcgc ccaggagcgc agacttagca
     2521 gcatattcca gcgacgatcg taagcgcctg ttgcctccag ccattcacga cgactggcag
     2581 aaagatccag ccgttgttgc tggatctggc taagcagttg cgccttacgt cgttcacgtt
     2641 cgactttact gctcacggga ctcctcctcg agcagctgcc gatcgtttgc cagctcatgg
     2701 cgtgtatggc gcagcaaggt ggatttacgc gatttacgta acgtccagat accgccaatc
     2761 agtgccagta gcagcaacac cacggtggtg gcaatcatcg cattcaggcg atattgcgga
     2821 tcaaccgccc aaataattag caccatcagg ctcataagac caaatgcagc gaaaagcatc
     2881 gtcaggccca gcatcagtaa aagttgaaag agattagctt tttcctcttc cagctcaacc
     2941 accgccagcc gcagacgtgt ttccaccatt tcaaccatga tggaaacaat tcgctgcccg
     3001 atgcccagaa cgcttttacc gggcccttgc gcgtgatgag tgtccgccat aattaacgac
     3061 gcgacagcag aacgccgagc actacaccga ttgcagcgcc aatgcccacg cccgtccatg
     3121 gattttcacg cacatactca tcggcacgcg ccgccgcgac acgggtttgt ttggcgatgg
     3181 catcaccggt ttcacccagg cgataacggc tctgtttcag tgcctgctcc gctttgctac
     3241 gaatcttact caactcttct ttcgacttct cgccagatga gctaagcacc tcttccagcg
     3301 tatcggaaag ggatttcaac tcagcacgca gatgttccgt agtgtgttct ttcgacatag
     3361 ttttctccag gtgagtaagt agtgactatt gtgagttagt agtcgcgcgc ttccagcttt
     3421 ttcagctctt cctgcgcttc tgccagtttc cgttcgcgtt tggcaatctt atcggcatcg
     3481 cctttttgct tcgcctcggc taaatcttgc tggcgttccg ccacctcatc tttctgcttt
     3541 gcgattttct tctgatgatc ggcacgcagc tggctatctg aacagttggc ccggacttca
     3601 ctcagggctt tattcagacc gtcaatacga ttctggtttt ggtgtttttc ggcatagctg
     3661 atctccttaa ggatattttg ctccttttcc tgacacaggg tagtggcata actaccggca
     3721 ctaagagcaa aaagagaaac agctaaagcg atgcggtatt tcattcttga accttccatt
     3781 gtgattcgga cctattacaa aggaacgatt gtccagacaa cgaagcggta gtgttaacca
     3841 ccgcctcatt aatcatagac agttacggtg aaatcaccaa agtgagtgat ctgtttcgga
     3901 caacaggaag ttatccgaag cgatgagagt tatcccgtag ccgggtcagc cactgcatag
     3961 cctgactgtt attgtcctgc tgcgcaatga tgtagccttg gggcaatgtt ctgtcgagga
     4021 ccgcttttgc agcggcgctc tggtcgctgg agtcaaaggt aattaacaga gtgtcgtttt
     4081 tgggggtgat acttttgaaa ggaatgccat gagcgtcaag gtgatgccag attgaaaaac
     4141 cgtctggcat cgttgtgcct tgatgaacgg cacgaatcgc cagcgtagac tcttgctggc
     4201 gaaccgcgga ccaggccagc aacagcgtgc cgaccagaag taaaacagcg ccggaccagg
     4261 ctagctggcg aagcgacatg cgaggtattt gcatccctta cccccgattt ccatatttct
     4321 ttttccataa tacgaccaga gaacctgcca ggccaaacac cagcagcacc accgggagca
     4381 gcatcaggca tgacatcagc tggtcctcgt actttaaaaa taccggcgtt ttgccgagca
     4441 tgtaacccag agttgtcagg atcaataccc acagcagacc gctcatccag ttgaaaaact
     4501 gaaagcgcgc gttattcagc cctgataacc cggcaatcgt cggcagcagt gttctgacaa
     4561 acgcaataaa gcgaccaatt aacagcgccg ataaaccgtg tttatgaaaa agatggtgtg
     4621 cgcgttgatg ataatgcgcg ggtaaatgag atagccagtt ttgtacggtg cgggtattgc
     4681 ccagccatcg cccctgaata tagctgaccc agcagccgag gctggcggca acggtcagca
     4741 gcagaatcgt ttgcggatag cccatcgcgc ctttcgcaat caacacgccg accaatacca
     4801 gtaaactgtc gcccggtaaa aaggccgccg gaagcaagcc gttttcaagg aacaaaatta
     4861 caaacaagac aaaatacaac atgccaatca tcgatggatt ggccagggtt tcaaaatcct
     4921 gcgcccacag ggcttgcagc aattgggtca aaagttccat tcattattcc tggtgaatcg
     4981 gttaacgcca cgctggtttt aaaagatgca ctaccgcaat gttattgctg ttttggtatg
     5041 ttctgcgggt ttgatacatc aacacagcct gaactattcc ctgttaaatc gtgagataag
     5101 aaagcattca aaaacataaa ttcaaattga atctaattgt aacaaaggtc aacgttgttc
     5161 gttatcaaaa ttacatgtcc gtggagtctg attttgctga atgcagggga gggtggcgag
     5221 aggatttaca caggctgaca ctttatactt ttcgcttacc gacgagacaa acaggcgcgt
     5281 cgcagcgcct gtcggacagg tatttcattt actgccactg gtggccgtat cgagatgcac
     5341 taccgggttt tcggcgaaca gatagcggtc ggcattgaac tcgaagtcgt cgctggtttc
     5401 gttgaataac atgatcttgg tgttttccag gtgctgccac attgccagct tagcagcatg
     5461 aggatctttg cgaatcagcg ccttgaggat ttgatcgtgg tcatcacacc agttatcgac
     5521 ggtacgggaa tcaatgtgtt cgtgcagttt tttccagtac gggttatggc tacgctgggt
     5581 ccacattttt tcaacgatag ccgccagggc ggagttctgc gtcgccaggg ctacctgaat
     5641 atggaactgc aaatcccact cggaatcacg gaagcattgt tcgccgcgcg cctgctcctg
     5701 gatggccatc aatttcatga tgtcctgttt cgttacctga gttgctgcga actcggcaat
     5761 attactttcg atgagttggc gagcctggag taactcaaac ggaccgtaat tggcgaattc
     5821 catattattg tccgcagcct gctgatggcg cggctggttg gaaaccacgt gaatacctga
     5881 acctttacgc acttcgacat agccttcaac ttccagcatg atgatggctt cacgaactac
     5941 cgtacggctg acgttctttt catcggcaat aaagcgctca gcaggcagtt tatcacccac
     6001 cagatagacg ccctgttcga tgcgctcttt caggtcagcg gcaagttgtt gatacaaacg
     6061 gcgtggttca gtgatttcca tatgcgctcc aggcatgata cggcagacta tatttgttat
     6121 accacttttg cgagtttagc tctactcgca gtgatgaaaa agccgcctga gaaggcggct
     6181 ttgaagcgaa actaaaactt acacgaagcg ttaatgttgc ggtgcgggat cgttatgtgt
     6241 ttcctgcgcc acctcaatcg ccggtttgtt ctgcaatacg gtccagataa ccagcgcacc
     6301 cagcaggtca aatactgcca gaactgcgaa cagcgggctg aagccgatgg tgtcagccag
     6361 tgcaccgaca accagcgcaa acagcgtact tgccagccat gcggacatcc cggttaaacc
     6421 gttcgccgtt gccacttcgt tacgaccaaa cacgtcggaa gagagcgtaa tcagcgcgcc
     6481 agacagtgcc tggtgggcaa aaccaccgat acacagcagc ataattgcga catacgggtt
     6541 ggtgaacagg ccgatcatac ccgggccaat catcagcact gcacccagcg ttacgaccat
     6601 cttacgggaa acgatcaggt tcacaccaaa ccaacgctgg aacagcggcg gcaggtaacc
     6661 accgaggata caaccgaggt cagcaaacag catcggcatc caggcgaaca tcgcgatctc
     6721 tttcaggtta aagccgtaaa ctttaaacat gaacagcggg atccacgcgt taaaagtacc
     6781 ccaggccggt tctgccagga aacgcggcag cgcgataccc cagaactgac ggttacgcag
     6841 gatctgacca acggacattt tcttcgccgt actcacctgg tgctgggctt cctgaccatt
     6901 aataatatag tcgcgttctt catcggtcag atgcttctgg tcgcgcggat gtttatagaa
     6961 aatcagccat gccatcgccc agataaagct caatgcaccg gagatgataa atgccatctg
     7021 ccagctgtgc atcacgattg cccataccac cagcggcggc gcaatcatcg caccaatcga
     7081 agaacctacg ttaaaatagc ctactgcgat ggaacgctct ttcgccggga accattcgga
     7141 gctggctttc agacccgccg ggatcatcgc tgcttccgcg gcaccgaccg caccacgagc
     7201 aacagccagg ccaccccagc tacctgccag cgcggttgca ccacagaaca cggcccacag
     7261 cacagcaaac attgcataac cgattttcgt acccagcaca tccagtacat aacctgctac
     7321 cggttgcatg accgtataag cagcagaata ggctgcgatg atataggaat actgttgggt
     7381 ggagatgttt aactcttcca tcagagttgg cgcagctgcc gccacagtgt tacgcgtcag
     7441 gtaaccaagc acggtgccga gcgtcaccag tgcgatcata taccaacgta accctttaat
     7501 tttacgcatg taaaacctca tcgttttgtt atatccgcag gaagcgcggc catcatcccg
     7561 ccagaggctg aagatgttag ttacgaccgg gtaaccgaaa aaaaaccggc acgccccgaa
     7621 cgttgccatg aaagtttcgt gctaattcgg cttccgtacc ggttaacccg attcatctaa
     7681 caaatgaatg aatcggcaat ttgcattccg tcgacttatg atttgcgacg gcagaaagat
     7741 aacttgtcat acaactttaa aaggtgagag ccatcacaaa tgtgcgaata tttgtaggga
     7801 cattacctga cgacagcgag gccagtactg gcgcggcctg cagagagatt taccactttg
     7861 agagtaattt ttttaactac gtttattgat ctaactcacg aaaatatctt cggactctgg
     7921 aaattggtgt gataactttg tcagcatcgc accataagca agctagctca ctcgttgaga
     7981 ggaagacgaa aatgactccg tttatgactg aagatttcct gttagatacc gaatttgccc
     8041 gccgtctgta tcacgactac gcaaaagacc agccgatttt cgattaccat tgccatttgc
     8101 cgccgcagca gattgcggaa gactatcgtt ttaaaaacct gtatgacatc tggctgaaag
     8161 gcgatcacta caaatggcgc gctatgcgta ccaacggtgt ggccgagcgt ctgtgtaccg
     8221 gtgatgcgtc tgaccgtgaa aaatttgacg cctgggcggc gactgttccg cacactatcg
     8281 gcaacccgtt ataccactgg acgcacctcg aactgcgtcg tccgtttggt atcactggca
     8341 aattgctttc tccgtcaact gccgatgaaa tctggaacga atgtaacgaa ctgctggcgc
     8401 aggataactt ctccgcgcgc ggcatcatgc agcagatgaa cgtgaaaatg gtcggcacca
     8461 ccgatgaccc gatcgattct ctggagcatc acgcagagat cgccaaagac ggctctttca
     8521 ccatcaaagt gctgccgagc tggcgtccgg acaaagcctt caacatcgaa caggcgacct
     8581 ttaacgacta catggcgaag ctgggcgaag tttccgatac cgacattcgc cgctttgctg
     8641 acctgcaaac tgccttgacc aaacgtctgg atcacttcgc cgctcacggc tgtaaagtgt
     8701 ctgaccatgc gctggacgtg gtgatgtttg ctgaagcgaa cgaagcggaa ttggacagca
     8761 ttctggcgcg ccgtctggct ggcgaaaccc tgagcgagca cgaagtggca cagttcaaaa
     8821 ctgcggtact ggtgttcctt ggtgccgaat atgcacgtcg cggctgggta cagcagtacc
     8881 acattggcgc gctgcgtaat aacaacctgc gtcagttcaa actgctgggg ccggatgtag
     8941 gctttgactc catcaacgac cgtccgatgg cagaagagtt gtctaagctg ctgagcaagc
     9001 agaacgaaga aaacctgctg ccgaaaacca ttctgtactg cctgaacccg cgcgataacg
     9061 aagtgctggg caccatgagc ggtaacttcc agggcgaagg tatgccgggc aaaatgcagt
     9121 tcggttccgg ctggtggttt aacgatcaga aagacggtat ggaacgtcag atgacccaac
     9181 tggcgcagct cggtttgctg agccgctttg tcggtatgct gactgacagc cgtagcttcc
     9241 tgtcatacac ccgtcacgaa tacttccgcc gcattctgtg ccagatgatc ggtcgctggg
     9301 tggaagcggg cgaagcaccg gcggacatca acctgctggg cgagatggtg aaaaatattt
     9361 gctttaacaa tgcgcgtgac tacttcgcca ttgaactgaa ctaaggtctg gggttgatat
     9421 gcaatacatc aagatccatg cgctggataa cgtcgcggtc gctttagcgg atttggctga
     9481 aggcacagaa gtcagtgtcg ataaccaaac tgttaggctg cgccaggatg ttgctcgtgg
     9541 acataaattt gcgttaacga atatcgcaaa aggggccaac gtcatcaaat atggcctgcc
     9601 gattggttat gcattggcgg atattgcggc gggggaacac gttcacgccc acaatacgcg
     9661 cacgaatctg agcgatctgg atcagtatcg ctatcaacct gattttcagg atctgcctgc
     9721 gcaagcggca gatcgtgaag tgcagatcta tcgtcgcgct aacggcgatg tcggggtgcg
     9781 taacgagctg tggatcctgc caaccgtggg ctgtgtcaac ggcatcgcgc ggcagatcca
     9841 gaaccgtttt ctgaaagaga ccaacaacgc tgaaggtacc gacggcgtgt tcctcttcag
     9901 ccacacctac ggctgctcac agctgggcga cgatcacatt aatacccgca ccatgctgca
     9961 aaacatggtg cgccacccga acgcaggcgc agtgctggtg attggtctgg gctgtgaaaa
    10021 caaccaggtt gccgcattcc gtgaaacgct gggcgatatc gatcctgaac gcgttcattt
    10081 catgatctgc caacagcagg atgatgagat cgaagccgga atcgagcatt tgcatcagct
    10141 gtataacgtg atgcgcaacg ataaacgcga gccaggcaaa ctcagcgaac tgaagtttgg
    10201 tctggagtgc ggtggttctg acggtctttc tggtattact gctaacccga tgctggggcg
    10261 tttctctgac tacgtgattg ctaacggcgg tactaccgta ctgaccgaag tgccggagat
    10321 gtttggcgca gagcagttgc tgatggacca ttgccgcgac gaagcaacgt ttgaaaaact
    10381 ggtcaccatg gtcaacgact tcaaacagta ctttattgcc catgaccagc cgatctatga
    10441 aaacccatcg ccggggaaca aagcgggcgg tatcaccacg ctggaagata aatcccttgg
    10501 ctgtacccag aaagcgggtt ccagcgtcgt ggttgacgtg ctgcgttacg gcgagcgtct
    10561 gaaaacgccg gggctgaact tgttaagtgc gccgggtaac gatgccgtag cgaccagcgc
    10621 cctggcgggt gcgggctgcc atatggtgct gttcagtact ggtcgtggca cgccatacgg
    10681 cggctttgta ccgacggtga aaatcgctac caacagtgaa ctggcggcga agaaaaaaca
    10741 ctggatcgac tttgacgcgg gtcagctgat ccacggtaaa gcgatgccgc agttgctgga
    10801 agaatttatc gataccatcg ttgagtttgc caacggtaaa caaacctgca acgagcgtaa
    10861 cgacttccgc gaactggcga tcttcaaaag cggcgtaacg ctataaagtc gaaatgagtt
    10921 gttaataaaa aacggcgttt cataatgtga agcgccgttt gcttttccat acctgataag
    10981 gatttatcat gaccgcgtat tggctggccc agggcgtggg tgtcatcgcc tttctgattg
    11041 gtatcacaac atttttcaat cgtgacgaac gtcgcttcaa aaagcagctt tcggtctata
    11101 gcgccgttat tggcgtacat ttttttcttc tgggcaccta tcccgctggt gccagtgcca
    11161 tccttaatgc cattcgtaca ttgattacct tacgcacgcg cagcttatgg gtaatggcga
    11221 tttttattgt gctgactggc ggaattggcc tcgcgaagtt ccatcatcct gtcgaactat
    11281 tgccggttat cggtacgatt gtcagtacct gggcgctgtt ccgctgtaaa gggctcacca
    11341 tgcgctgcgt gatgtggttt tcaacgtgtt gctgggtgat tcacaacttc tgggcggggt
    11401 cgataggcgg cacgatgatt gagggtagtt ttctgcttat gaatggcctg aatatcattc
    11461 gtttctggcg gatgcagaaa aggggaattg atccgtttaa agtagagaaa acccctcccg
    11521 ccatagacga aaggggttaa acaattaatt acgcagggcg ctatttgcca gacgatcgtc
    11581 ttccgcctgg caagctgccg cagtgaacag cacgtcagtt gaagagttca gcgcggtttc
    11641 gcaagagtcc tgcaatacgc cgataataaa gccgacggca accacctgca tggcgatatc
    11701 gttcgaaata ccgaacatat tacaggccag tgggatcagc agcaaagaac cccccgccac
    11761 gccggatgcg ccacaggcac acagagaagc cactacgctc aacagcagcg ctgtgggcag
    11821 atcgaccgga atacccagcg tattaaccgc agccagcgtc aacacggtaa tggtgattgc
    11881 tgcgcccgcc atattgatgg tggctcccag cggaatagaa acggaatagg tatcgcgatc
    11941 cagattcagc ttttcacaca gtgccatatt caccggaatg ttcgccgcag agctgcgggt
    12001 gaagaaggca tacacgccgc tttcgcgcag gcacagcagc accagcggga acgggttacg
    12061 acgaattttc caccacacca gcaatgggtt aaccaccagc gccaccagta acatacagcc
    12121 aaccagcacg accagcagtt gcgcgtagcc ccacagtgtg gagaaaccgg tggttgccag
    12181 ggtagaagaa accagaccaa aaataccaat cggtgcgaag cgaatgacca gtttcaccat
    12241 aaaggtgacg gcattcgaca tatcgttgac caggtttttg gtggtctcgt taccgtgacg
    12301 cagtgcgaaa ccgaggccaa ttgcccacac caggatcccg atgtagttac ctttcagcag
    12361 cgcgtcgatg gggttggaaa ccatgctcat taccagcccg cgcatcactt ctacaatgcc
    12421 tgacggtggc gaaatatcac ccgcgctact ggataagtgc agggtagaag ggaaggcgaa
    12481 gctgaaaacg actgcggcca gagcagcaga gaaggtaccc agcagataga ggaacaaaat
    12541 agggcggata ttggttttct gcccgtgctg gtggttagca atagatgcca tcaccagcat
    12601 caacaccagg atgggggcaa cggctttcag tgcgccgacg aacaaagtac ctaacagacc
    12661 aacagcttcc gccgcgggtt ttgagatcca tgccagaaga atccccagaa caaggccgac
    12721 caggatttgt tttaccaggc tgccatgagc cagacgccgg aatagccccg gtgaacgttg
    12781 cgtagtcatt tttcgatcct ttcattgtgt tgtcgcacat ccctggtgcg ttctgtcgac
    12841 gcatctttca ggatgtaaca aagtgtttgc aaggtcgagt ataaggaaag ttacagcttc
    12901 gggaagcgaa aaatgctgga ttttaaagag aggtcatatt ttgcaactta atggttacat
    12961 ttatttgaca taaaaataaa gccaacgtgc ggcttccaga ctgaggacta cgtcaaaaaa
    13021 ggactcggtc agtcccctcg ccccgtttgg ggagagggtt agggtgaggg gaacaggcca
    13081 gcatcggtgc gaaaaattac cctcaccccg accctctccc tggaagggag agggagaaaa
    13141 cggtgctcat gttgatgaca gggtacacgg cttaggcaga ttaaaaatca tccaacccgc
    13201 tgcttatcat gccgataatt cacccaggcg ttgataataa acgtcatcac caggatgcca
    13261 aacaccacgc ccagcgagac ggcgattgga atatggtaga agtcgacaat cagcatcttg
    13321 ataccgataa acaccagaat caccgccagg ccatatttga gcatcgagaa acgctctgct
    13381 acgcccgcca gcaggaaata catcgcacgc aggccgagga tcgcaaacag gtttgaggtc
    13441 agcacaatga acgggtcagt ggtcacggcg aagatagccg gaatgctatc cacggcaaaa
    13501 atcacgtcgc tcaattccac cagaatcagt accagcatca gcggtgtggc gtacagcaat
    13561 ccattcttac gcacaaagaa atgctcgttg tcgatggtgt cggtcatgcg caaatgaccg
    13621 cgtagccagc gcaccagcgg cttgtcgcca atgcctgatt catcttcatg ggcgagcgcc
    13681 atcttcacgc cggtaaacag caggaaggca ccgaagatat acaggatcca gtcgaactgc
    13741 gaaatcagcc agctgccagt gaagatcatg atggtacgca gaacaatcgc cccgagcacg
    13801 ccatacacca gcacgcggcg ttgtaatgcc gccggaacgg agaaatagct gaacaacatc
    13861 agccagacaa agacgttatc gaccgccagc gacttctcaa tcagataacc cgtgaggaag
    13921 gccagcgcct gtggatcggc aacggcgcga ccttcggttt gtaccagata ccaccagaaa
    13981 gcagcgttaa acagtaacga cagcgtcacc cagaccagcg accaggccgc agcctgtttc
    14041 atggtcatgg catgtgcccc acgacgtccc tgcaacaaca ggtcgatagc cagcataatg
    14101 gcgacaacaa cagcgaatcc gccccatagc aacggcgtgc cgacagtatt catagaagtt
    14161 ccttacacat aaaaaaaacg gccaacgtcg gaagacgtca gccgctgctt tttatgcata
    14221 gacctcgcct tccggcaagg tctcacttac aacaaaaaag gaatacgtct gcgtattcct
    14281 ttcgggttgc ccggtgaccg gatgtggttt ttcacacatc gtaatgacga tcgaccggca
    14341 atgaagttac tcccctttgc aggtaacaaa gtatgacaga ccattcagtc agtcaatggt
    14401 ctgttacata ccttttacac tgttttactt tggcaattat agttttacgc tatctgccgg
    14461 aaaaatcacc ccagtctggc ggcggatctc ggtcagcagt ttggctgtga tgcgactgac
    14521 cgccagccct ggatgatcca ccagatgctc atccaccagg gtagcgaaca gctctgcttc
    14581 gtagagcatg gtattaatat gctgcggctg ggtgagatcc tgcatctggc tgccacgcgg
    14641 cacgaagcac actttctggc attcagacag tttttcaatc accagcgatc ccgcttcgcc
    14701 ctgaatctcg cttgccagga cagaatcgct gacctttgag tgctgcaagg tgacgctgaa
    14761 atcaccgtaa tccatcacca ccacgccgtg ggcatctaca ccacttgcca gtaaactggc
    14821 ggttgcctgc acgcttttcg gctcaccaaa taacgctacc gccgacgcca ggcagtaaaa
    14881 gccgatatcc ataattgaac cgttagagaa tgccggatta aaggtgttgg gattctcacc
    14941 gtccaggtaa cgttgatacc gcgaggaata ctggcaatag ttgaaaaaaa ctttccgcag
    15001 tttgccgact ttcggcagcg cctggcgtaa cagatgaaag ttaggcaggc tggccgtttt
    15061 aaatgcctca aacagcacca cctgattttc acgcgcgcag gcaatggctg catccacttc
    15121 cgccagattc gacgccagtg gtttctcaca aatcacatga attttgtggc tcaggaaaag
    15181 ttgcgtctgg gaaaaatgca gggcgttcgg gctggcaata tacaccgcgt cgatagcgtc
    15241 actttccgcc attgcttcca gcgaagtaaa cagatgctcg acagaaaact cattggcgaa
    15301 gtgctgggcc tgttcaaggc tgcgggaata aacggcggtt aacttatatt taccgctttc
    15361 atgtgcggcc tccacgaact ggtgagttat ccagttcgta ccaatcacgg cgaaacgtat
    15421 cataacgcgt ggatactcca taaagggatt ctgtcgtcaa tgtagcacgc ctttttcatc
    15481 ttcgacgtgc gcggatacgc attatttctg cgccggacaa ccatgctcac taaacggacc
    15541 aataatgacc gacggcccga gatattttgg ctgacgattg aagattttcg cgtcaatcac
    15601 cgcgctgact ctggcgagtt tctctcgcag acggacaatc tttccgcgtc ctttttccag
    15661 gtctggtgcg ttaaacccga tagcatcgat gccgtattgt tttgccagcc agatggcgcg
    15721 ttggttatga aattcctgcg agataatggt gatatggttc tccccaaaca ctttattggc
    15781 acgcaccact gaatcaagcg ttgagaatcc ggcatagtca cagaagatca cttttgccgg
    15841 tacgcccttt gcgatcaaag cctgctgcat gcctgacgct tcatcgtaat ttttacgtcc
    15901 gttatcgcca ctgaccagca gccatttcac ttttccggcg tggtacaact ctgctgcggt
    15961 atcaatacgt cgggtaaagt agcgattacc cggcctggcc cccagcaata agcccacgtt
    16021 acgcgccgga acgacgttga cgtcgctcca ggtcagctgt ttgctggcgt tgaccattac
    16081 gcgatcggcg ataaaaatgg tcgctcctgc cacaagtaac agtaagcagg caatctttag
    16141 agtccggcga gaaaaacaaa tacggagaag aaggcgggca aatgcgcgca gcatggtcgt
    16201 tatccttaac gaaagggggg aggtcgattt taggcgctaa agaaagaaaa gataacgcct
    16261 gacatcattt tcagattttg tcctgcccaa gcgacaactg ggttagccac agtcgggtgt
    16321 cgaactcaag ctggtggtac tggggttcca tatgacagca caactggtaa aacgctttgt
    16381 tatgctcttt ctccttcagg tgcgccagct cgtgcaccac gatcatgcgc aaaaacggtt
    16441 ccggcgcgtt gcgaaacacg gtagcgacgc ggatctctgc ttttgctttc agcttgccgc
    16501 cctgcacacg agaaacagcg gtatgtaacc cgagcgcgtt ttttagcaca tggatcttat
    16561 tgtcatacat cactttattg atcggcgggg cattacgcag aaactgattt ttcagatcct
    16621 gagtatattg ccagagggct ttatcggtgg cgtagtcgtg tgttcccgga tagcgttttg
    16681 ccagcacatc gcccagacgc tgttcgttaa tcagtgttcg cacctgggaa agtagctgct
    16741 cgggataacc ctgaagataa gtaagattgc tcataaccgc ccacgaaaaa gagaaaaagg
    16801 gtatactcac gcaccctttt caggggcaat gtcgaaattt tatcattcag gagggccgat
    16861 gagccactta gacaacggtt tccgttcact gacactacaa cgttttccgg cgacggatga
    16921 cgttaacccg ctacaggcgt gggaagcggc ggatgaatat ttgctgcaac agttggacga
    16981 cacagaaatc cgcggcccgg tgttgatcct gaatgatgcc tttggtgcgt taagctgcgc
    17041 gctggcggaa cataagccgt acagcattgg cgactcatac atcagtgaac tggcgacgcg
    17101 cgagaattta cgcctcaacg ggattgatga gtcgagcgtg aagtttctcg acagcaccgc
    17161 cgactacccg caacagccgg gtgtggtgct gatcaaagtg ccgaaaacac tggcattgct
    17221 ggaacagcaa ctgcgtgcgc tgcgcaaagt ggtcacgcca caaacacgta ttattgccgg
    17281 tgccaaagcc cgtgacatcc acacttccac gctggaacta ttcgaaaaag tgctcggccc
    17341 gaccaccacc acgctggcat ggaagaaagc gcgcctgatt aactgcactt tcaatgaacc
    17401 accgctggcc gatgcgccgc agaccgttag ctggaagctg gaaggtactg actggactat
    17461 ccacaaccat gcgaatgtct tctcccgcac cgggctggat atcggcgcgc gcttctttat
    17521 gcaacatctg ccagagaatc tcgaagggga gattgtcgat ctcggttgcg gtaatggcgt
    17581 tattggtctg acgctgcttg ataaaaaccc gcaggcgaaa gtggtgtttg tcgatgaatc
    17641 gccgatggcg gttgcttcca gccgtttgaa cgttgaaacc aacatgccag aggcgttgga
    17701 tcgctgcgaa tttatgatca acaacgcgct ctccggcgtg gagcctttcc gctttaatgc
    17761 tgtgctctgc aacccgccgt ttcatcagca acatgcgctg accgataacg tcgcctggga
    17821 gatgttccac cacgcccgcc gctgcctgaa aatcaacggc gagctgtata tcgttgccaa
    17881 ccgtcacctg gattacttcc ataaactgaa gaaaattttc ggcaactgca ccaccatcgc
    17941 cacgaataat aaatttgtgg tgctgaaagc agtgaagctg gggcgtcgtc ggtaagattg
    18001 acagctacct gccagataag gcgtaaacgc cttatctggc atacgtttag cacattaccc
    18061 ccaggtcgga taagatgcgg caagcgtcgc atccggcaat gtctgcccag ttctggaatc
    18121 gcgctccata aaacaaaaaa tccctgtcac catcctctac attctcttgt ttagcgttgt
    18181 ttaacgttta ttcttccgtc acacagataa attcatccgt tgccaatctg tcaacggatg
    18241 ttatcatatt gccaatttgt caacggacgt gatgaagaat gcacctgata actcaaaaag
    18301 cattgaaaga tgctgcggaa aaatacccgc aacataaaac ggagttggtg gctctaggga
    18361 acacgattgc taagggatat ttcaaaaaac ctgagtcatt aaaagcagta ttcccatctc
    18421 tggataactt caaatatctg gataagcatt atgttttcaa tgttgggggc aatgaattac
    18481 gtgttgtagc aatggtcttt tttgaatcgc aaaagtgcta catacgtgaa gttatgacgc
    18541 ataaagaata cgattttttt accgctgttc atcgtactaa ggggaaaaaa tgattgcgat
    18601 tgccgacatc ttgcatgcag gagaaaagct aactgctgtg gcaccttttc tggcgggtat
    18661 tcagaacgag gaacaataca tccaggcgct ggaactggta gatcatctgc tgctcaacga
    18721 tcctgaaaac cccttactgg atctggtgtg tgccaaaata accgcgtggg aagaatcagc
    18781 gcccgaattt gcggaattta atgccatggc tcaagccatg cctggcggta tagccgtgat
    18841 tcgtaccctt atggatcaat atggtttaac cctttccgat ctgccggaaa ttggcagtaa
    18901 atctatggtg tcacgcgttt tgagcgggaa gaggaaatta acgctggaac acgctaaaaa
    18961 attggcaacg cgattcggca tttctcccgc cttgtttatt gattaacg
//
LOCUS       DAZKIP010000082        18848 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZKIP010000082 DAZKIP010000000
VERSION     DAZKIP010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.082"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            18..443
                     /gene="nudI"
                     /locus_tag="ACHOUK_003169"
     CDS             18..443
                     /gene="nudI"
                     /locus_tag="ACHOUK_003169"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFP9898562.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLAHYDLNVATRKTLRLKGLL"
     gene            complement(482..1084)
                     /gene="ais"
                     /locus_tag="ACHOUK_003170"
     CDS             complement(482..1084)
                     /gene="ais"
                     /locus_tag="ACHOUK_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFP9898563.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLGRLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            1392..2531
                     /gene="arnB"
                     /locus_tag="ACHOUK_003171"
     CDS             1392..2531
                     /gene="arnB"
                     /locus_tag="ACHOUK_003171"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFP9898564.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNHALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLQGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADINAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENIARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPSLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            2535..3503
                     /gene="arnC"
                     /locus_tag="ACHOUK_003172"
     CDS             2535..3503
                     /gene="arnC"
                     /locus_tag="ACHOUK_003172"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFP9898565.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHILVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREYGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            3503..5485
                     /gene="arnA"
                     /locus_tag="ACHOUK_003173"
     CDS             3503..5485
                     /gene="arnA"
                     /locus_tag="ACHOUK_003173"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFP9898566.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHHCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            5482..6372
                     /gene="arnD"
                     /locus_tag="ACHOUK_003174"
     CDS             5482..6372
                     /gene="arnD"
                     /locus_tag="ACHOUK_003174"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFP9898567.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKYHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            6372..8024
                     /gene="arnT"
                     /locus_tag="ACHOUK_003175"
     CDS             6372..8024
                     /gene="arnT"
                     /locus_tag="ACHOUK_003175"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFP9898568.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLTIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            8021..8356
                     /gene="arnE"
                     /locus_tag="ACHOUK_003176"
     CDS             8021..8356
                     /gene="arnE"
                     /locus_tag="ACHOUK_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFP9898569.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAISKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            8356..8742
                     /gene="arnF"
                     /locus_tag="ACHOUK_003177"
     CDS             8356..8742
                     /gene="arnF"
                     /locus_tag="ACHOUK_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFP9898570.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(8736..9002)
                     /gene="pmrD"
                     /locus_tag="ACHOUK_003178"
     CDS             complement(8736..9002)
                     /gene="pmrD"
                     /locus_tag="ACHOUK_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFP9898571.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASCYSPDEWERQCKVAGKTQ"
     gene            complement(9112..10467)
                     /gene="menE"
                     /locus_tag="ACHOUK_003179"
     CDS             complement(9112..10467)
                     /gene="menE"
                     /locus_tag="ACHOUK_003179"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFP9898572.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AVQGVVEDSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHVATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQTEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVMEYDHESVDLSEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(10464..11426)
                     /gene="menC"
                     /locus_tag="ACHOUK_003180"
     CDS             complement(10464..11426)
                     /gene="menC"
                     /locus_tag="ACHOUK_003180"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFP9898573.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRTAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(11426..12283)
                     /gene="menB"
                     /locus_tag="ACHOUK_003181"
     CDS             complement(11426..12283)
                     /gene="menB"
                     /locus_tag="ACHOUK_003181"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFP9898574.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(12298..13056)
                     /gene="menH"
                     /locus_tag="ACHOUK_003182"
     CDS             complement(12298..13056)
                     /gene="menH"
                     /locus_tag="ACHOUK_003182"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFP9898575.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCLEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILSFWLVGYSLGGRVAMMAACQGLAG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRSEPLTAVFADWYQQPVFASLNDDQ
                     RRELVVLRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(13053..14723)
                     /gene="menD"
                     /locus_tag="ACHOUK_003183"
     CDS             complement(13053..14723)
                     /gene="menD"
                     /locus_tag="ACHOUK_003183"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFP9898576.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIACRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETVLADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(14812..16107)
                     /gene="menF"
                     /locus_tag="ACHOUK_003184"
     CDS             complement(14812..16107)
                     /gene="menF"
                     /locus_tag="ACHOUK_003184"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFP9898577.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIAHHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(16186..16491)
                     /gene="elaB"
                     /locus_tag="ACHOUK_003185"
     CDS             complement(16186..16491)
                     /gene="elaB"
                     /locus_tag="ACHOUK_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFP9898578.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(16546..17007)
                     /gene="elaA"
                     /locus_tag="ACHOUK_003186"
     CDS             complement(16546..17007)
                     /gene="elaA"
                     /locus_tag="ACHOUK_003186"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9898579.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEIYEEDGIPHIGMAREVIQA"
     gene            17072..17989
                     /gene="rbn"
                     /locus_tag="ACHOUK_003187"
     CDS             17072..17989
                     /gene="rbn"
                     /locus_tag="ACHOUK_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFP9898580.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPSPLFQELKAGKTIMLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            18177..>18848
                     /locus_tag="ACHOUK_003188"
     CDS             18177..>18848
                     /locus_tag="ACHOUK_003188"
                     /EC_number="3.4.19.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElaD/SseL family deubiquitinase"
                     /protein_id="HFP9898581.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAEDILYHIVRNPTNDTLSIIKLIKNACLKLYNLAHTATK
                     HPLKSHDSDNLLFKKLFSPSKLMAIIGEDIPLISEKQSLSKVLLNDKNNELSDGTNFW
                     DKNRQLTTDEIACYLKKIAANAKNTQVNYPTDFYLPNSNSTYLEVALNDNIKSDPSWP
                     KEVQLF"
BASE COUNT         4551 a         4996 c         4699 g         4602 t
ORIGIN      
        1 ttataaggaa tacccctgtg cgacaacgga ctattgtatg ccctttgatt caaaatgatg
       61 gtgcttattt gctgtgtaaa atggccgacg atcgcggcgt tttccccggt caatgggcgc
      121 tttcgggtgg cggcgtggag cccggcgaac gaattgaaga ggctctacgc cgcgaaattc
      181 gcgaagaact gggagaacag ctgcttttga cagaaatcac gccctggacc ttcagcgatg
      241 atattcgcac caagacgtat gcagatggtc gcaaggaaga aatttatatg atttacctga
      301 tttttgattg cgtttctgcc aaccgagaag tgaaaataaa cgaagagttt caggactacg
      361 cgtgggtaaa acctgaagat ctggcgcact atgatttgaa tgtcgccacc cgaaaaacgt
      421 tacgtttgaa aggtcttctg taacaatgat gacagccatt actggctgtc agattgaaaa
      481 tttagtgatt aacgaattcc ccatccagat aaactttgcc tttttccaca tgcatgacta
      541 aaccatccag ataatcaggt ttaaatgtcg catcacgctt atctttagca atatatgtca
      601 ggcaatgatt atgggtgaaa ataacgatat ttttatcagg cgctttgctt tgtaagtcct
      661 taatcgcact ataaatctca ttaccgcact gaagaagtcg tttatctacc gtcaattttt
      721 tacccgctga aaaccaggtc gcggactgaa tggtccggac ggtattactg gaataaagat
      781 cgaaatcagg gatatcagca ctaaaagcgt tgcccagttc acgggcatcc tgggtacctt
      841 taaccgtaat acctgtttta tctgacaagc attgattggt tgaacgatcg caacgttcag
      901 catggcgaaa caaaacgaca accgggtgct gctgtgccag tctgcccagc gttttattgt
      961 cgatacgtgg caaaccattg ctactccagg cggcatgagt acccagtccg gcaattgcag
     1021 cgagcgccag taaaatgatg aaatattttt ttgacttcaa tgatgagcgg caaaaagcta
     1081 acacgtaaac tccacctata gacaagcgca accagacaat taccgtgaaa ttgagcgaca
     1141 tttctggcga taattagcag ttggtgtaat attaaaaatc ctacgatgct ggcaaaatgc
     1201 ctcaaaattt tgccaaatgc aaagcctaaa taagaaaaaa tataaaaatt tcaatattta
     1261 cgtctaatat tagtttctta aggttaagtt aatattctat ccttaaaatt tcgctctaaa
     1321 tggcaaaata tacacaacac tctttatagc aaatataagt ggacaggtat tcaatggcgg
     1381 aaggaaaagc aatgtcagaa tttttgcctt tttcgagacc agcaatgggc gtggaggaac
     1441 tcgctgcagt taaagaggtt ctcgaatccg gttggatcac aaccggtccg aagaatcacg
     1501 cgcttgaaca agccttttgc cagttgacgg gaaatcagca tgccatcgcg gtcagttcag
     1561 ccaccgccgg aatgcatatc acgctaatgg cgttggaaat tggcaagggc gatgaagtga
     1621 ttacgccttc cctgacctgg gtttcaaccc tcaatatgat ttccttgcag ggtgcaacgc
     1681 cggtaatggt ggatgtcgat cgcgatacgc tgatggtcac gcctgaagcg atcgaatcag
     1741 ccattacgcc acgcactaaa gccatcattc cggtgcatta tgccggtgcg ccagcagata
     1801 ttaacgccat tcgcgccatt ggcgaacgtt acggcatcgc agttatcgaa gatgctgccc
     1861 atgccgtagg tacgtattac aaagggcgac atattggcgc aaaaggtacc gctatttttt
     1921 catttcatgc cattaaaaat attacctgtg cggaaggagg cctgattgtt actgataatg
     1981 aaaatattgc ccgccagcta cggatgctga aatttcacgg cctgggtgtc gatgcctatg
     2041 acagacaaac ctggggccgt gcaccgcagg ctgaagtctt aacgccgggc tataagtaca
     2101 atctgaccga tattaacgcc gcgattgccc tgacacagtt agccaaatta gagcacctca
     2161 acacccgtcg gcgcgaaatt gcccagcaat atcagcaagc actggcagct ctcccctttc
     2221 agccattaag ccttcccgcc tggccgcacg ttcacgcctg gcatctgttt attattcgtg
     2281 tcgatgaaca acgttgtggt atcagtcgcg atgcgttgat ggaagcgtta aaagaaagag
     2341 gtattggtac cgggttacat ttccgcgccg ctcacacaca aaaatattat cgcgagcgtt
     2401 ttccctcgct gtcgttaccg aataccgaat ggaatagcga acgcatctgt tctttgccgc
     2461 tgttcccgga tatgactacc gccgatgccg accgtgtcat cacagccctt cagcaactcg
     2521 caggacaata agccatgttt gaaatccacc ctgttaagaa agtctcggtg gttattcccg
     2581 tttataacga gcaggaaagc ttaccggaat taatcaggcg caccaccaca gcctgtgaat
     2641 cgttggggaa agagtatgag atcctgctga ttgatgacgg cagtagcgat aattccgcgc
     2701 atatactggt cgaagcctca caagcggaga acagccatat tgtatctatt ttgcttaacc
     2761 gcaattacgg gcaacattca gcgattatgg cgggattcag tcacgttact ggcgacttaa
     2821 ttattaccct tgatgccgat ctccagaatc cgccagaaga aatcccccgc ctggtggcaa
     2881 aagccgatga aggttacgac gtggtaggga ctgtacgcca gaaccgccag gacagctggt
     2941 ttcgtaaaac agcttcgaag atgattaacc ggcttattca gcgcaccacc ggcaaagcga
     3001 tgggtgacta cggttgtatg ctgcgcgcct atcgccgtca tattgtcgat gcgatgttgc
     3061 actgccatga acgcagcacc tttatcccga ttctggcgaa tatcttcgcc cgccgtgcca
     3121 ttgaaattcc agtgcatcat gccgagcgtg agtatggtga atccaaatac agctttatgc
     3181 gcctgattaa tttgatgtac gacctagtga cctgccttac cacaacgccg ctacgtatgc
     3241 tgagtctgct cggcagcatt attgcgattg gaggttttag cattgcggtg ctgctggtga
     3301 ttttacgcct gaccttcgga ccacaatggg cggcagaagg cgtctttatg ctatttgccg
     3361 tgctgtttac ttttattggc gctcagttta tcggcatggg attactcggt gaatatatcg
     3421 gcaggatcta caccgatgtc cgcgcccgcc cccgctattt tgttcagcaa gttatccgtc
     3481 catccagcaa ggaaaatgaa taatgaaaac cgtcgttttt gcctaccacg atatgggatg
     3541 cctcggtatt gaagccctgc tggctgccgg ttacgaaatt agcgccattt ttacccatac
     3601 cgataatccc ggtgaaaaag ccttttatgg ttcggtggct cgtctggcgg cggaaagagg
     3661 cattccggtt tatgcgccgg ataacgttaa tcatccgctg tgggtggaac gcattgccca
     3721 actctcacca gaggtgattt tctcttttta ttatcgccat cttatttgcg acgagatttt
     3781 gcagctcgct cccgcaggtg catttaatct gcacggttcg ctgttaccaa aatatcgtgg
     3841 tcgcgcgccg ctgaactggg tgctggtgaa cggtgaaaca gaaactggcg ttacattgca
     3901 ccgaatggta aaacgtgccg atgccggggc cattgtggcg caactgcgcg ttgccattgc
     3961 gccagacgat atcgctatta cgctgcatca taaattgtgc catgccgcgc gccagctact
     4021 ggaacagaca ttacccgcca ttaaacacgg taatattctg gaaatcgccc agcgcgaaaa
     4081 cgaagccacc tgttttggtc gcagaacgcc ggatgacagt ttcctcgaat ggcataagcc
     4141 ggcatccgta ctgcacaaca tggtacgtgc cgttgccgat ccgtggccgg gtgccttcag
     4201 ctatgttggc aatcagaaat tcaccgtctg gtcgtcgcgt gttcatcctc atgccagcaa
     4261 agcacagccg gggagcgtga tttctgttgc gccactgctg attgcctgtg gcgatggcgc
     4321 gctggaaatc gtcaccggac aagcgggcga cggcattact atgcagggct cgcaattagc
     4381 gcagacgctg ggcctggtgc aaggttcacg cttgaatagc cagcctgcct gcaccgcccg
     4441 acgccgtacc cgggtactca tcctcggggt gaatggcttt attggcaacc atctgacaga
     4501 acgcctgctg cgcgaagatc attatgaagt ttacggtctg gatattggca gcgatgcgat
     4561 aagccgtttt ctgaatcatc cgcattttca ctttgtcgaa ggcgatatca gtattcattc
     4621 cgaatggatt gagtatcacg tcaaaaaatg tgatgtcgtc ttgccgctgg tggcgatagc
     4681 cacgccgatt gaatataccc gcaacccgct gcgcgtattt gaactcgatt ttgaagagaa
     4741 tctgcgcatt atccgctact gcgtgaagta ccgtaagcga atcatcttcc cgtcaacttc
     4801 agaagtttat gggatgtgta gcgataaata cttcgatgag gaccattcta atttaatcgt
     4861 cggcccggtg aataaaccac gctggattta ttcggtatca aaacaattac ttgatcgggt
     4921 gatctgggcc tatggcgaaa aagagggttt acagttcacc ctcttccgcc cgtttaactg
     4981 gatgggacca cgactggata accttaatgc ggcacgaatc ggcagctccc gcgcgattac
     5041 gcaactcatt ctcaatctgg tagaaggttc accgattaag ctgattgatg gcggaaaaca
     5101 aaaacgctgc tttactgata ttcgcgatgg tatcgaggcg ttataccgca ttatcgaaaa
     5161 tgcgggaaat cgctgcgacg gcgaaattat caacattggc aatcctgaga acgaagcgag
     5221 cattgaggaa ctgggcgaga tgctgctggc gagcttcgaa aaacatccgc tgcgccatca
     5281 tttcccaccg tttgcgggct ttcgcgttgt cgaaagtagc agctactacg gcaaaggata
     5341 tcaggacgta gagcatcgta aaccgagcat ccgcaatgcc caccactgcc tggactggga
     5401 gccgaaaatt gatatgcagg aaaccatcga cgaaacgctg gatttcttcc tgcgcaccgt
     5461 tgatcttacg gataaaccat catgaccaaa gtaggcttac gcattgatgt cgataccttt
     5521 cgtggcaccc gtgaaggcgt gccgcgtctg ctggaaatct tgagtaagca taatattcag
     5581 gccagcattt ttttcagcgt cggcccggac aatatgggcc gccatctctg gcgactggtg
     5641 aagccacagt ttttgtggaa gatgctgcgc tcaaacgcgg catcgcttta tggctgggat
     5701 attttactgg caggtacggc ctggccaggt aaagagattg gtcatgccaa tgccgatatc
     5761 attcgtgaag cggctaaata tcacgaagtc ggcctgcacg cctgggatca ccatgcctgg
     5821 caagcccgta gcggtaactg ggatcggcaa acaatgatcg acgatattgc acgcggcctt
     5881 cgcactctgg aagagattat cggtcaaccg gtaacctgtt ctgccgctgc gggctggcgt
     5941 gccgaccagc aggtgatcga agcaaaagaa gcgttccatt tgcgctacaa cagcgattgt
     6001 cgtggggcca tgccgttccg tccactgctc gaatcaggaa accctggcac tgcgcaaatt
     6061 ccggtgacct tacccacctg ggatgaagtg attggtcggg atgtgaaagc agaagatttt
     6121 aacggttggt tactcaaccg catcctgcga gataaaggca cgccggttta taccattcat
     6181 gcagaagttg aaggctgcgc ttatcagcat aattttgtgg atctcctcaa acgcgcagct
     6241 caggaaggcg tgacattttg ccctttaagc gaactgttat cagagacgtt gccgctcgga
     6301 caagttgttc gcggaaatat tgccggacgt gaaggctggc tgggttgcca acaaattgcg
     6361 ggtagtcgct gatgaaatcg gtacgttacc ttatcggcct cttcgcgttt attgcctgct
     6421 attacctgtt accgatcagc acgcgtctgc tatggcaacc cgatgaaacg cgttatgcgg
     6481 aaatcagtcg agaaatgctg gcatccggcg actggattgt gccccatctg ttagggctac
     6541 gttattttga aaaacccatt gccggatact ggattaacag cattgggcaa tggctatttg
     6601 gcgcgaataa ctttggtgtg cgggcaggcg ttatctttgc gaccctgtta actgccgcgc
     6661 tggtgacctg gtttactctg cgcttatggc gcgataaacg tctggctcta ctcgccacag
     6721 taatttatct ctcattgttt attgtctatg ccatcggcac ttatgccgtg ctcgatccgt
     6781 ttattgcctt ctggctggtg gcgggaatgt gcagcttctg gctggcaatg caggcacaga
     6841 cgtggaaagg caaaagcgca ggatttttac tgctgggaat cacctgcggc atgggggtga
     6901 tgaccaaagg ttttctcgcc cttgccgtgc cggtattaag cgtgctgcca tgggtagcga
     6961 cgcaaaaacg ctggaaagat ctctttattt acggctggct ggcggtcatc agttgcgtac
     7021 tgacggttct cccctgggga ctgacgatag cgcagcggga gcctgacttc tggcactatt
     7081 ttttctgggt tgagcatatt caacgctttg cactggatga tgcccaacat agagctccgt
     7141 tctggtacta cgtgccggtc atcattgccg gtagtctgcc gtggctggga ttactccccg
     7201 gtgcactata cacaggctgg aaaaaccgca agcattccgc aaccgtctat ttgttgagct
     7261 ggacgataat gccgctgctg tttttctccg ttgctaaggg taaattgccc acctatattc
     7321 tttcctgctt tgcacctttg gcaatgctga tggcgcatta cgctttgctg gcagcaaaaa
     7381 ataatcctct ggcgctgcgg attaatggct ggattaacat cgcttttggc gtcactggca
     7441 ttattgccac atttgtggtc tccccctggg gaccaatgaa cacgccggtg tggcaaacct
     7501 tcgagagcta taaagtcttt tgtgcctggt cgattttttc gctatgggca tttttcggct
     7561 ggtacacctt aacaaacgtc gaaaagacct ggtcttttgc cgcgctttgc ccgctggggc
     7621 tggcgttgct ggtaggattt tcaattcctg acagagtcat ggaaggaaaa catccgcaat
     7681 tttttgtcga gatgacacaa gaatcacttc agccaagccg ctatattctt actgatagcg
     7741 tcggtgttgc cgcaggtctg gcatggagtc tgcaacgcga tgacatcatc atgtatcgcc
     7801 agacaggtga gttgaaatac ggccttaatt atccggatgc gaaagggaga tttgtcagcg
     7861 gtgatgagtt cgcaaactgg cttaatcaac atcgtcagga ggggattatt acactcgtgc
     7921 tttcggttga ccgcgatgaa gatatcaaca gtctcgccat tccgcccgca gatgccatcg
     7981 atcgtcagga gcgtctggtg ctgattcagt atcgtcccaa atgatctggc taacattagt
     8041 ctttgccagc ttgcttagcg ttgccgggca gttgtgtcag aaacaggcaa cctgctttgt
     8101 ggcgataagc aaacggcgca aacatatcgt gctgtggctg ggactggcgc tggcttgtct
     8161 tggtcttgcc atggtgctct ggctgctggt cttgcagaac gtaccggtag gtattgccta
     8221 cccgatgtta agtctgaatt ttgtctgggt gacgctggct gcagtaaaac tgtggcacga
     8281 accggtatcg ccgcgtcact ggtgtggggt ggcgttcatt attggtggca ttgtgatcct
     8341 cgggagtacg gtgtaatggg cctgatatgg ggattattca gcgtcattat tgcctcagtt
     8401 gcgcaattaa gcctgggttt tgcggcgagt catctgccgc cgatgacgca cttatgggat
     8461 tttatcgcgg cgctgttagc ttttgggctg gatgcccgga tactgttact cgggctgctg
     8521 ggttatctgc tttccgtttt ttgttggtat aaaacactac ataaacttgc gctaagcaaa
     8581 gcctatgcct tgctcagtat gagttatgtg ctggtatgga ttgcttcaat ggtacttccc
     8641 ggctgggaag ggactttttc gctgaaagca ctactgggag tagcctgtat tatgagcggg
     8701 ttgatgctga ttttcctgcc cacgacaaaa caacgttact gagttttccc tgccacttta
     8761 cactgccgtt cccactcatc ggggctataa cagcttgcag agaggacttt caccgtatgc
     8821 agcttttcac gattaataca ataaagcgca ttctgcaaag gcgagagtaa atcccccact
     8881 ttcacggcga agtcactctt cacttcggcg atcattttta tcgcgccgcc agcatcgcag
     8941 agcataagta catgtctgtt atcttgttta ttgcagcacg attttttgac cagccattcc
     9001 attgtattat cctgtttgct aagagttttc atatcaattt acagattaac agataaactc
     9061 tttatcaacg gcaatggaat ggcagaactg agaagtgaaa cggagggtta attattgctg
     9121 acgttgcacc cactctttta gcgcctgacg tgaaatttta ataccgccgt ttttcagctc
     9181 cggcggcaaa gtcagccaac gtaccggttg ctgaaaacgg gccagcttat ctttcaccca
     9241 ttcactaaga tcaacgctct cgtggtcata ctccatcacc gccaccggtc gatgaccaaa
     9301 ctccttgtcg gcaacgggga cgataaacac ctgcaaaacc gcaggatgtg cagcaattac
     9361 gcgctcgact tcttccgtct gaataccctc tccgccactg aagaatagat tgtctaaacg
     9421 tccgacaatg gtcagcttgc cattatgcat ctcaccgcga tcgcgcgtag cgtaccagcc
     9481 ttcgtcatta accagtgaaa ccagttgccc gttacgccag taaccttctg ccatactggc
     9541 agcccgcagc cacacttcat tattaacgat tttcacttcc cgacccggca gcggcgaacc
     9601 aacgtctgcc aggccgtcgg cttctttcgc acacaccgtg gaggcaaact cggtcagacc
     9661 atagccgcaa aagcaacgaa tcccctgctc gcgcgcctgt tccgtcaact cgaccgggat
     9721 agccgcgccg ccaagtaaca ccgctttcag ggaaacggaa ctgcggttaa ccagcaaacg
     9781 ccagagttgt gttggcacca gtgaagcgtg agtacagcct gccagcattt gctccaatgg
     9841 ctgtttatca cgcaccgtca tccgcgcacc agcgtataac cagcgccaca taattccctg
     9901 accggagacg tgaaacagcg gtaaagagag caaccagtca tcgtgatcgc caaacggaat
     9961 cagcgataac accccttcgg cactggcaag atgggcctga taagtatgga cagcggcttt
    10021 cggcaacccg gtagaacccg aggtcaacgt cattgagcac agacgcgtcg gctgccacgt
    10081 agcgacatgt gcgccttcaa cccgctgaat gtgcagcgac gttaatgccg gaaacgtgtt
    10141 ttccccatcc ggcaccagcg caaattgcag cgtcagattg ggcagcaatt cttcaagcaa
    10201 cggttgcggc agctgagggt tcacgggcaa cacccgcgcc ccgcattgca gtaacgccag
    10261 ccaagcgagc agcgtttgcg gcgtattcca cgcccgcaac atcacgccgc tgtcctcaac
    10321 cacaccctgc accgcaaatc cggaggctaa ttcatcgacg cgagcacaaa gctcgcgcca
    10381 gttgagttgc tcgtcattaa gacgtaaggc gatggcttct ccccgcactt gccgccagtg
    10441 acgccacggc cagtcagaga agatcataac aaccgctcca gtgcatcaac ttccacgaca
    10501 ggcagcgtgc tacccggcca gcgacgtacc tgctgcgcct gcattagatc cagcgtgtcc
    10561 agccctggaa tggtgtccgg cgttaaccag gcagcaatcc gcgccagttg cgttaagcct
    10621 aagctcgatt caatggaaga actgatcact gccgtcagcc ccagcgcgtg cgccgcctgt
    10681 acctgctcgc gaactttctc cagactgccc gtgagcgtgg gtttgataac taccgcgcgc
    10741 acgccctctt cagccacaaa ggcaaaatcc ggctcgcgca agctttcatc ccaggcaatg
    10801 gcaatgccgg tttcacgggc aaacgctcgc gaatcatcgc gggttttgca cggctcttcg
    10861 agaaacgcga tgcggtggcg ataatccggg ttaacgtatt tggcaaactg ctgacctttc
    10921 agcggtgtcc aggcgcgatt tgcgtcaaga cgcaaatgca gatccggaat tgcctccagc
    10981 aacagattca ccaccatgcc gtcgcgcacc gcttcgtaca atcccacttt gaccttcgcc
    11041 actttctcgc ctggcatatc tgcaagtttg aggatcagat cgtccggatc gccattacac
    11101 agtggtgccg tacggtaatt ggccgcctgc gggagcgtat ctgccagctc cgccaatgca
    11161 caacttacgc caaaggcaac ggaaggcatc tgcggtaatt cgcaatcgcc tgccagccag
    11221 ttatttaccc aggcaagcag cacactttgc gcctcttccc aggtttcctg actgaagccc
    11281 ggcagtgggg agatctcccc ccacccttcg cgctcgccct cacgcaggca aacgtacagc
    11341 ccgtcgcggg tttttaaccg cctgtcgcgc agaaccaccc ccgcgtccat ggggatctgc
    11401 cagcggtata cctgcgcgct acgcattacg gattccgttt gaatttgctg aagtcaggct
    11461 gacgtttctg gttgaaggcg ttgcgacctt cctgaccttc ttccgtcatg tagaacagca
    11521 tggtggcgtt gcccgccagc tcctgcagac ctgcctgacc gtcacagtcg gcgttcagtg
    11581 cagctttcag gcagcgcagc gccatcggac tgttttgcag catttcacgg caccagcgaa
    11641 cggtttcttt ttccagatcc gccagcggta ccacggtgtt gacaaggccc atatccagcg
    11701 cctgttttgc gtcgtactga cggcacagga accagatttc acgcgctttt ttctgcccga
    11761 cgatacgagc catgtaggaa gcgccccagc cgccgtcgaa ggaaccgact ttcgggccag
    11821 tctgaccgaa gatggcatta tctgccgcga tagtcaggtc gcacatcatg tgcagaacgt
    11881 gaccaccgcc gatggaatag ccagccacca tcgcgacaac cggtttcgga caggtacgga
    11941 tctgacgctg gaagtccagc acgttcaggt gatgtacgcc ggaatcatct ttatagccgc
    12001 cgtaatcacc acgcactttc tggtcaccac cggagcagaa cgctttatcg cctgcgccag
    12061 tcagaatgat cacgccgacg ttatcgtcat aacgcgcatc tgccagtgcc tggatcatct
    12121 cttttaccgt cagaggacgg aaggcattgc gcacctgcgg acgattaatg gtgatttttg
    12181 cgataccgtc ggtggatttt tcataacgaa tgtcctcaaa gccttcggag cagtcgtgcc
    12241 attcaaccgg tgcgtaaagc attgcttcat caggataaat catattgtgt cctttagtca
    12301 gaaacgcaag atctgcgcca gacttgcgat tacgccagcg ggattttccc gatgcgcgtt
    12361 atgtccggcg cgaggaatga catggcagtc ggcagccagt tccgccgcca gggcgcggaa
    12421 tttgctgtca cgttcaccac ataaataata aaacgcaaat gtgcgggcgc taaggttagc
    12481 acgtaaatca ggctggacgg cgagagaagt cgcctccagc atggcggcaa gggttgcgcc
    12541 attattgttg ctgcgcagca ccaccagctc ccggcgttga tcgtcattga gcgaggcaaa
    12601 aacaggctgt tgataccagt cggcaaatac cgcggttaat ggttcggaac gaaaacgctg
    12661 cgcccattgg cgatcggaac gctgacgttc cgcgcgttgt tcagcatttt gcagccccgg
    12721 atgaccgcct tcgacaacaa ccccacaaag ccccgccagc ccctggcaag cggccatcat
    12781 cgccacccgt ccgccaagcg agtaccccac cagccagaag ctaaggatgt tgtaactaac
    12841 cagggtttta cgcagtaagt cggtgacatc atcaaatcca tcgacgctaa tcgccgccga
    12901 accaccgtga cctgggagat caacatacaa ccgtgagtag tcggcaaacg cctcgccgac
    12961 ttcctgccat tcgaggcaat cgccggaaaa accgtgcaaa aacaccagcc agggtaaacc
    13021 tggtttgccg tgttttgcct gcgcgtgcag gatcataaat ggcttacctg cgccagcagc
    13081 tgctggagcg tttgcgcgcc gtcggtgtcg ttaaccacca tttcaattac cgtggtggtc
    13141 ggagtacgcc aggcgtcggc aagtaccgtt tcaagttcct gccagttttg cggacgatga
    13201 tatttcagct caaacatcgc ggcagcgtgc tcaaaatgga cgttttgcgg catcagatag
    13261 aaacgctcgc gctcgctttt cggcgttggc agcagcgaga aaatttgccc gccgttgttg
    13321 ttcaccacaa ttaataccag cggcgcggaa acctggcgca ataacgccag cgcattcagg
    13381 tcgtaaagtg cggagagatc gcccacaatc gccagcgtcg gtttgccgct tgcccgctga
    13441 acgccggcgg cagtcgagag cagcccgtcg ataccgctgg ccccacggtt gctgtacacc
    13501 gggtaacctg ccggaagttg cgaaagcgca tcaatcagac gtaccaccag gctgttaccg
    13561 acaaacaatt gcccctgttc aggcagatag tcgctgatgc gatgcgccag ttgcgcttcg
    13621 ccaaacgcat cgcgacaggc aataaccgcc tgcattgcct gttccgccag gcgcgggatt
    13681 tcaacgcacc agggctggcg tttttctgcc ggatgcagtt ccagccagtc ggcaatattg
    13741 gcaattaagc ggcgtccacg atggtgtgcc ggatcaagcc gcccttcgat gtcatccaca
    13801 atccagtact cttcgggttc acagcttgcc tgccattgca ggagccgttt gcccgtcagg
    13861 ctgcttccca gttgcaccac aatttgcgcc tgttgcagtt cgctggtcgc tttggcattg
    13921 cctaaccaga gatcggcaca cggcagcggc tgtccggttt gcgacagcac gtcgccaatc
    13981 agcggccagc caagagtttg cgcccacagg gcaacttttt tgccctcttc cgcactcatg
    14041 cgcccggcaa ccaccacgcc gcgcttttgc cgccagaaga accagtcgcg ctgtttttcg
    14101 ctctcaagac gaggcgcttc acgcagccac ggcttatcat cctgccacca gtcacccaga
    14161 cgctgttgcc agctaagccc agtgtcgtcc atctcgccat acagcggttc agcaaacggg
    14221 cagttgatat ggaccccacc agcatgaagc gtaccgagag catggtcgat ggtagaaacc
    14281 agccaacgtg cggggatatc ctgggtcggg cgcggcagtg aaatactgtg cgtggggtga
    14341 gaggcgaaca ttcccggctg acgaatcgcc tgattcgcgc cgcagtcaat tagctccggc
    14401 gggcgatcgg cggttaacag gatcagtttt tctccggtta acccagcttc aatcagtgcc
    14461 ggatagagat ttgctaccgc cgtgccggag gtcacaatca ccgccaccgg ctgcttgctg
    14521 actttcgcca gccccagcgc cagatgcccc agtccacgct catcgaaatg ggtgtggtga
    14581 atgaatgcgg aattctccgc cgccgctaac gttaacggtg tagaacgcga gccaggggcg
    14641 atgcagatgt gtcttacgcc gtgacgcgtt aatgcttcca gaatgaccgc cgcccagcgt
    14701 cggttaaatg cgcttactga catgagtttg tccggtatca atattgcggc taagtataag
    14761 gagctacaaa aatagattat tgatatgaat cggtaatgat gcgactcatt actattccat
    14821 ttgtaataaa gtacgcagcc ctgccgcttt gttgtcgatt tcctgccact cttgctcggg
    14881 gtcggaacca cggacaatgc ccgcgccagc atatagccgc acgacattgc cgctgatttt
    14941 tgctgagcgc agggaaacgc agaattcgct ttgttgtaat gagagatagc ccgcagaacc
    15001 ggcgtaccat tctcgggtga acggttcgtg atgggcgata aactgtcgcg ccagatcgcg
    15061 cggtaagcca gcaactgctg ccgttggctg caactgatgt aaacagatca catcatccgc
    15121 tttgttgagt gaagtccaga tacagcggcg aagatgctgc actttacgca gacgcagtac
    15181 ctgcggcggt aaaacatcca gcgtctgggt atcggcctgt aatcgttggc agatatcttc
    15241 caccaccagc atgttctcgc gctggttttt atcatccgcc atcagccact ctcctaactg
    15301 ctgcgcctgc ttatcatcag gatgatttgc tactgttccc gccagcgctt cagtacgcag
    15361 cgctttgtca cgccgccgcc ataaccgttc cggtgaagag ccaagaaaag cattttcgcc
    15421 atcaaaggcc atgtaaaaat ggtagcaatt gagattcagt cgacgactgg cagccatcat
    15481 cgccgccgca ttgaccggac ttgcgaaatg caggtcagtt gcccgagcaa gcaccacttt
    15541 gtcgagctca ccttcggcga tggttttcgt tgccagttcg attaattgcg tccagcccgt
    15601 tttgtccggc cagtgttgtt ctcgcgtagt ggttaaatgt aacccaggca agggcttgat
    15661 actcaccagt gtggcgataa attctcttgc cttaatcgca tcgtgctgaa gggagctttc
    15721 gctgaatagc gtcagccgca gcgtggcttt accgccacag cgtcgccatt ccaggcgtgg
    15781 taaaagtaaa ttgccctgcg acgggtcaaa cgcattcagc ccccaaatgc gtaagtcggc
    15841 gtgttccggg tgctggcgaa gaaaacgttg tgcctggtcc aacgacgtaa aacgggtaat
    15901 cgcgcccagg acggcagctt cttcatcacc attacgttgt tgccagtaga attgcgggta
    15961 cgtttgctga ctggccagcc agctcaaggc atcaaaagcg tctttgagag ggaaaggaat
    16021 atcgataacc cgaatgccgg gtgtcgccgg aatctcttgc gacaaatggc gcagtagatt
    16081 ttccagcgcc gtagtaagtg attgcacgcg gacctctccc tgttaaaagc ctcacattat
    16141 acggggtact acaaaaaaaa tgcagtaccc cggtgtaggg aggttttaac ggcgtgccag
    16201 caacagtcct agtaccagcc caacggccgc acccacacca attccctgcc agggtttttc
    16261 gtggacgtag tcatcagcac gataaacagc ctgcttcgcc cgatagtaat aactgtctga
    16321 agcctggcta acccgttttt tcacatcatc cagcgctttt tctgcacgcg ctttcagctc
    16381 aacatatttc tgatcggcgg gatcgccaga ggagcggagc acctcttcca gtgtttcact
    16441 aagcagcgtc aggtcgtcat cgatacgtgt atcaccaaac tgattagaca ttctcgttct
    16501 ccatttgcgt aaaacctgtc actaactata gacaagggtt attgattacg cctgaattac
    16561 ctcgcgtgcc atcccaatgt gtgggatacc atcttcttca tagatctccg tcacagggat
    16621 aaaaccgaaa ctctggtaga agttttgcag atgcgcctgc gccccgaggt agacaggctt
    16681 atcaggccag tgatgcgtgc aagtgtcgag ggttttactc atcagttgct gccccacttt
    16741 ttcgccacgc aacgcttcac tgacaatcac ccgaccaata acgaccggct caagatcatc
    16801 atcacttttc agaatcctcg catatgccac cagttcatca tttttccaac cgagaatatg
    16861 gcgattatcc cccgtcaaat catcaccatc gatatcctga taagggcaat tttgctcgac
    16921 aacaaacacc gcgcaacgca gttgtaataa ggcataaagc tgggaaaccg ataattcaga
    16981 gtgatgcaga tcttgccatt caatcataat gctctccttt tagatgtatg ccttatacta
    17041 gccggaatat tttttgaaac gtgatgaact catggaatta atttttttag gtacttcggc
    17101 cggtgtgcca acccgcacgc gcaatgtcac ggcaatattg ctaaacctgc aacatccgac
    17161 ccagagcgga ctttggttgt ttgactgcgg tgaaggcacc cagcatcagc tactgcatac
    17221 cgcctttaac cctggaaaac tggacaagat ttttatcagt caccttcatg gcgatcatct
    17281 ttttggttta cccggcttgc tgtgcagtcg ttctatgtca ggcattatcc aacccttaac
    17341 gatttatggt ccgcaaggta tccgtgaatt tgtggagacc gcgctgcgga ttagcggctc
    17401 atggaccgat tatccgctgg aaattgtcga aattggcgct ggcgaaattc tcgatgatgg
    17461 cctgcgcaaa gtgaccgctt atccaatgga acacccactg gaatgctatg gctatcgtat
    17521 tgaagaacat gataaaccgg gtgcattaaa tgcccaggca ttaaaagctg ctggcgtacc
    17581 gcctagccca ctgtttcagg aattaaaagc gggcaaaaca atcatgctgg aagatggaag
    17641 gcagattaac ggcgcagatt acttagctgc tccagtgcca ggtaaagcgc tcgctatttt
    17701 cggcgatacc ggcccctgcg atgccgcact tgacctggct aaaggtgtcg atgtcatggt
    17761 gcacgaagcg acgctggata taaccatgga agccaaagcc aatagtcgcg gtcatagctc
    17821 tacacgccag gctgcgacac tggcccgtga ggctggagtc ggtaagctaa tcattaccca
    17881 cgtcagctcg cgctatgatg acaaaggttg tcagcacctg ttacgtgaat gcaggtcaat
    17941 tttcccggcg actgaactag cgaatgattt caccgtgttt aacgtttaac tctgcttatt
    18001 attaaaaaca gggcgaaact tgccctgtta tcgcaacccg cgctacatat atccactcac
    18061 acgggcatat tgagcgattt ttgatattta ttcatctcct tacgcactat attcagagca
    18121 ataagttgcc tctttttatc ttcattcagc acaacctttg caactaaaag gagcatatga
    18181 tggttacagt tgtcagcaat tattgtcaat tatctcaaac ccaactcagt cagacatttg
    18241 cagaaaaatt tactgtgacc gaggaattac tgcagtcttt aaaaaaaaca gcgttatccg
    18301 gagatgaaga aagcattgag ttactgcata atattgcgtt aggttatgat gaatttggga
    18361 aaaaagctga agatattctt taccatattg ttagaaaccc aacaaatgat accctatcga
    18421 ttatcaagct tataaaaaat gcctgtttaa aattatataa tttggcacat accgcaacca
    18481 aacaccccct caaatcacat gattcagata atctcctgtt caaaaaacta ttctcccctt
    18541 cgaaattaat ggcaattatc ggtgaggaca ttcctctcat atcagaaaaa cagtcacttt
    18601 caaaggtact tttaaatgat aagaataatg aactgagcga tgggacaaac ttctgggata
    18661 aaaatcgtca attaaccaca gatgaaatag cttgctatct taaaaagatc gccgccaatg
    18721 caaaaaatac tcaagtcaat tatcctactg atttctacct ccccaattcc aacagcactt
    18781 acctggaagt cgctctcaat gataatatta agagcgatcc atcatggccg aaagaagtcc
    18841 agttattc
//
LOCUS       DAZKIP010000083        18610 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZKIP010000083 DAZKIP010000000
VERSION     DAZKIP010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.083"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(7..630)
                     /gene="rsmG"
                     /locus_tag="ACHOUK_003189"
     CDS             complement(7..630)
                     /gene="rsmG"
                     /locus_tag="ACHOUK_003189"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFP9898582.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(694..2583)
                     /gene="mnmG"
                     /locus_tag="ACHOUK_003190"
     CDS             complement(694..2583)
                     /gene="mnmG"
                     /locus_tag="ACHOUK_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFP9898583.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSDDKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(2962..3405)
                     /gene="mioC"
                     /locus_tag="ACHOUK_003191"
     CDS             complement(2962..3405)
                     /gene="mioC"
                     /locus_tag="ACHOUK_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFP9898584.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(3495..3953)
                     /gene="asnC"
                     /locus_tag="ACHOUK_003192"
     CDS             complement(3495..3953)
                     /gene="asnC"
                     /locus_tag="ACHOUK_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFP9898585.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4105..5097
                     /gene="asnA"
                     /locus_tag="ACHOUK_003193"
     CDS             4105..5097
                     /gene="asnA"
                     /locus_tag="ACHOUK_003193"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFP9898586.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(5102..6553)
                     /gene="viaA"
                     /locus_tag="ACHOUK_003194"
     CDS             complement(5102..6553)
                     /gene="viaA"
                     /locus_tag="ACHOUK_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFP9898587.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(6547..8043)
                     /gene="ravA"
                     /locus_tag="ACHOUK_003195"
     CDS             complement(6547..8043)
                     /gene="ravA"
                     /locus_tag="ACHOUK_003195"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFP9898588.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPASLQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHNMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQRCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            8266..10134
                     /gene="kup"
                     /locus_tag="ACHOUK_003196"
     CDS             8266..10134
                     /gene="kup"
                     /locus_tag="ACHOUK_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFP9898589.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANQEVLYALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            10301..10720
                     /gene="rbsD"
                     /locus_tag="ACHOUK_003197"
     CDS             10301..10720
                     /gene="rbsD"
                     /locus_tag="ACHOUK_003197"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFP9898590.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKHHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            10728..12233
                     /gene="rbsA"
                     /locus_tag="ACHOUK_003198"
     CDS             10728..12233
                     /gene="rbsA"
                     /locus_tag="ACHOUK_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFP9898591.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFESCDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            12238..13203
                     /gene="rbsC"
                     /locus_tag="ACHOUK_003199"
     CDS             12238..13203
                     /gene="rbsC"
                     /locus_tag="ACHOUK_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFP9898592.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            13228..14118
                     /gene="rbsB"
                     /locus_tag="ACHOUK_003200"
     CDS             13228..14118
                     /gene="rbsB"
                     /locus_tag="ACHOUK_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFP9898593.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            14244..15173
                     /gene="rbsK"
                     /locus_tag="ACHOUK_003201"
     CDS             14244..15173
                     /gene="rbsK"
                     /locus_tag="ACHOUK_003201"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFP9898594.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            15177..16169
                     /gene="rbsR"
                     /locus_tag="ACHOUK_003202"
     CDS             15177..16169
                     /gene="rbsR"
                     /locus_tag="ACHOUK_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFP9898595.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(16135..17562)
                     /gene="mdtD"
                     /locus_tag="ACHOUK_003203"
     CDS             complement(16135..17562)
                     /gene="mdtD"
                     /locus_tag="ACHOUK_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFP9898596.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPILGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNHVPSESE"
     gene            complement(17585..18277)
                     /gene="yieP"
                     /locus_tag="ACHOUK_003204"
     CDS             complement(17585..18277)
                     /gene="yieP"
                     /locus_tag="ACHOUK_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFP9898597.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         4487 a         4612 c         4865 g         4646 t
ORIGIN      
        1 taaaaattaa attttatttg ctttaatcac caccagatga cgttcgccat ccagggctgg
       61 aacctgaagt ttaaccactg attcgacctg atattcttcg ggcaacaaag cgatttcatc
      121 ttccggcatt tgccctttca gcgcgtagaa acggccttgc tcaccaggaa gatggtggca
      181 ccagctcacc atatcgttca gagaggcaaa agcgcggcta attacgccat caaatggcgg
      241 ctctgaagga aactcttcta ccctgctctg tactggctca atattctcca gtttaagctc
      301 atgttgcacc tgacgaagga aacgcacgcg tttaccaagg ctatccaaca gagtgaaatg
      361 ggcttcagga cgcacgatag agagtggaat gccaggcagt ccaggaccgg tgccgacatc
      421 gataaaccgt tcaccttgca gatacggtgc caccacaatg ctatcgagaa tatggcgtac
      481 cagcatctca ttaggatcgc ggaccgaagt caggttgtac gctttgttcc atttatgcag
      541 catattcacg taggcaataa gctggttttt ctggtgatcg gtaagcgaaa tacctgcgtc
      601 tttcagcagt aaggagagtt tgttgagcac ggtgattacc tgttcttgat gcgttgcctg
      661 gtaagcgggg gcttaccagg catttttaat gcgttatgcg ctacgacgca gcataccctg
      721 ttttttcagc cacaccagca gaatggagat ggccgcaggc gtgacgccag aaatacgtga
      781 agcctggccg atagaggccg gtttgtgatc gttaagtttg gcgatcactt cgttagaaag
      841 accggatacc tggcggtaat ccagtgtcgc gggtagcaag gtgttctcgt tacgcagctg
      901 cttttcgatc tcatcttgct ggcgcgcgat ataaccttcg tatttaacct gaatctcaac
      961 ctgttccgcc gcctgttcgt ctgtcaacgc aggggcaaac ggcgtcagcg tggttaattt
     1021 ttcataagtc atttccggac gacgcagcag atcttcacca ctggcttcac gggaaagcgg
     1081 cgcagtcagg tgagcattca cttcggctgc agcttccgcc gacggggtta cccaggtcga
     1141 tttcagacgc tgacgctcac gctcgatatt ctcaagtttc tcgttaaagc gcgcccaacg
     1201 ttcgtcatcc accaggccca gttcacgacc gatttcagtc aaacgcagat ccgcattatc
     1261 ttcgcgcagc atcagacgat attctgcgcg cgaggtaaac atacgatacg gttctttggt
     1321 tcctaaagtg cacaggtcat caaccagtac gccgagatag gcctgagaac gcgccggagc
     1381 ccaaccttct ttgtcatcag acagacgggc agcgttaaga ccggccagca aaccttgcgc
     1441 agcggcttct tcgtaaccgg tagtgccgtt aatctgacca gcaaagaaca gcccctggat
     1501 aaacttgctc tccagcgtcg gtttcaggtc gcgaggatcg aagaagtcat actcaatggc
     1561 ataacccgga cgcacgatct tcgcgttttc catcccctgc atagagcgga cgatttgcat
     1621 ctgcacatcg aacggcaggc tggtggagat accgttcgga taaatttcgt tagaagtcag
     1681 cccttccggt tcaaggaaga tctgatgctg atttctgtcg gcgaagcgca tgactttgtc
     1741 ttcgatcgac gggcagtagc gtgggccgac accttcgatc acccctgcgt acattgggct
     1801 acgatcgagg ttactgcgga tcacatcatg ggttttctcg ttggtatggg tgatataaca
     1861 cggcacctgc tgtggatgct gggacgcatt gcccataaaa gagaataccg gcattgggtt
     1921 atcgccatgc tgttgcgcaa gcacgctaaa gtcgatggtt cgagcatcaa tacgcggtgg
     1981 tgtcccggtt ttcagacgac caacgcgcag cggaagttca cgcaaacggc gagaaagcgg
     2041 aatggacggc ggatcaccag cacggccacc gctgtagtta tccagaccga tatgaatttt
     2101 accgtcgagg aacgtcccaa cggtgagcac gacggctttg gcacggaact tcagtcccat
     2161 ttgggtaaca gcaccgacca cacgatcgtt ttcgacaata agatcttcaa ccgcctgctg
     2221 gaagatcatc aggttcggtt ggttctccag cgccgtacgt accgcctgac ggtagagcac
     2281 acgatccgcc tgagctcggg tagcgcgaac tgccggtccc ttgcttgcgt ttagtatcct
     2341 aaactggata cccgcctgat cgatcgcttt cgccatcaga ccgccgagtg catccacttc
     2401 ttttaccaga tgtcccttcc caataccgcc gatcgccggg ttgcagctca tctgccccag
     2461 agtgtcgata ttgtgtgtca aaagcagagt ctgttgaccc atacgcgccg cagccatcgc
     2521 ggcctcggtg cctgcatgac ccccgccaat gatgatgacg tcaaaaggat ccggataaaa
     2581 catggtgatt gcctcgcata acgcggtatg aaaatggatt gaagcccggg ccgtggattc
     2641 tactcaactt tgtcggcttg agaaagacct gggatcctgg gtattaaaaa gaagatctat
     2701 ttatttagag atctgttcta ttgttatctc ttattaggat cgcactgccc tgtggataac
     2761 aaggatccgg cttttaagat caacaacctg gaaaggatca ttaactgtga atgatcggtg
     2821 atcctggacc gtataagctg ggatcagaat gaggggttat acacaactca aaaactgaac
     2881 aacggttgtt ctttggataa ctaccggttg atccaagctt cctgacagag ttatccacag
     2941 tagatcgcac gatctgtata cttatttgag taaattaatc cacgatccca gccattcttc
     3001 tgccggatct tccggaatgt cgtgatcaag aatgttgatc ttcagtgttt cgcccgtctg
     3061 ttttgcaccg gaatttttga gttcagcctc gagtttatcg atagccccac aaaatgtgtc
     3121 atattcacga ctaccaatac cgattgcgcc aaagcggacc gcagaaagat cgggcttctg
     3181 ttcctgcaat gcttcataga aaggagaaag gttgtccgga atatctccgg caccgtgggt
     3241 ggagctgata accagccaga tccccgaggc agataaatct tccaacagcg gaccgtgcag
     3301 cgtttcggtg gtaaaacccg cctcttccag cttttcagcc aggtgttctg ctacatattc
     3361 ggcaccgccg agggtgctgc cgctgataag agttatatct gccataaacc gccaccttta
     3421 ttaagagtgg cgtattgtac gctgtgaacg cgttgggatc tacctgtgga aaagtatggg
     3481 attaaaaaag ccgatcaggg cttgatggta cgcatgatcg ggttctgcag gacgatcagt
     3541 gtctcggtgg actgaatttc atcaattgtt tggatcttgt tgataagtac atgctggaga
     3601 gcatcgatcg aacggcacat cacttttata aagatgctgt agtggccggt ggtgtagtac
     3661 gcctcggtca cttcatcgag gctttccagc tttgccagcg cggaagggta gtctttggcg
     3721 ctctttaata taatgccgat aaagcagcct acgtcataac cgagctgctt cgggctgaca
     3781 tcaatacgcg ccccggtaat gatccccgcc tgcttcattt tctctactcg aacgtgaatc
     3841 gtccccggac tgacgccaaa ttgtttcgcc agttcggcgt aagcggtgcg cgcattgccc
     3901 attaatgctt ccaggatgcc acggtccaga ttgtcgatca gataattttc cataggattt
     3961 tcttatgcgg attgatgatt cattctattt tagccttctt ttttaatgaa tcaaaagtga
     4021 gttaggcttt ttattgaatg attattgcat gtgtgtcggt ttttgttgct taatcataag
     4081 caacaggacg caggagtata aaaaatgaaa accgcttaca ttgccaaaca acgccaaatt
     4141 agcttcgtga aatctcactt ttctcgtcaa ctggaagaac gtctggggct gatcgaagtc
     4201 caggcgccga ttcttagccg tgtgggggat ggcacgcagg ataacttgtc gggctgtgaa
     4261 aaagcggtac aggtaaaagt gaaagctctg cctgatgccc agttcgaagt ggttcattca
     4321 ctggcgaagt ggaaacgtca gaccttaggg caacacgact tcagcgcggg cgaagggctg
     4381 tacacgcaca tgaaagccct tcgccccgat gaagaccgtc tttctccgtt gcactcggtc
     4441 tatgttgacc agtgggactg ggaacgcgta atgggcgacg gtgagcgtca attctcgact
     4501 ctgaaaagca cggtagaggc gatttgggcg ggaattaaag caaccgaagc tgcggttagc
     4561 gaagagtttg gcctggcacc gttcctgccg gatcagatcc acttcgtaca cagccaggag
     4621 ttactgtctc gttatccgga tcttgatgcc aaagggcgtg agcgggcgat agcgaaagat
     4681 cttggtgcgg tattccttgt cgggattggc ggcaagctga gcgatggtca tcgccacgac
     4741 gtgcgcgcac cggattatga tgactggagc accccgtcag agctgggcca tgcgggtctg
     4801 aacggcgata ttctggtgtg gaaccctgta ctggaagatg cgtttgagct ttcttccatg
     4861 gggatccgcg tggatgccga cacgttgaag catcagttgg cgctgaccgg tgatgaagat
     4921 cgtctgcagc tggaatggca tcaggcgttg ctgcgcggtg aaatgccgca gaccatcggc
     4981 ggcggtatcg gccagtctcg tttgaccatg ctgctgctgc aactgccgca tatcggccag
     5041 gtccagtgtg gagtatggcc agctgctgtt cgcgagagcg tcccttctct gctgtaataa
     5101 tttatcgccg ccagcgtctg agcaggcggc ttcgcatccc ggtatcaaag cgccagatat
     5161 gatcgaaaat gcgcatgatg ccgggtttgc cgtgtgccga catcgccacg gcatgaaagc
     5221 gatgctgatg tacccgctgc agctctttca ctttactcgt cacgtcgtca ggcaaccgct
     5281 gggcgataaa atcagaaatc accaccgcat cggcatcaaa ccattccctg ctttgcaagc
     5341 gttccataat ggcgcgaaaa caactggcaa gatcggtgcc gccacgaaac tgctggctta
     5401 aaaaacggat tgcttgttcg atgccttgtg ggcctgaaag ctcataacgg acgatctcgg
     5461 tggaaaatag cataatataa cagcgccggt tttcagcgag agcaatgcgc atcaaagcca
     5521 ggcagaacgc tttcgcacac tgttcattaa agccgcccat tgagccggaa gtatccacgc
     5581 agacaataaa cggtccgcgc ggctgttcgt cgtaatcttt atgcaccacc gggcgttcga
     5641 tcactttttc acgccacgac tcaccgtgca ggcgataggt gagcaactgt ttttccacca
     5701 gccgacggta aaactcatac tccagttccg ttatccctag tgtcgccagt tctggcggca
     5761 gaagacgtaa aatatcatcg ctttgttgca gaccatcaac ctgctcagga accgtcgccg
     5821 gttcgcgcac catggtgcgg aaggtttcca tctgcgcatc gttgcgcggt attgatttgg
     5881 cttcccggga acgccccagt tgttctgcca ggcgtttcag ttccggctgt tcgttaagaa
     5941 attcaccgta tttcacaatc aactgatagt cgccacgttt aagctgaccg gcgctcatat
     6001 cccacagacg accagctgcg gtattgttat ctgcgagaat cggttcaagt tgtccgctca
     6061 gcgtcatgcg ttcctgaact tcactcaaca gttgttcgcg ttcttcttct aatagctgtt
     6121 gatttaacgt tgttgcttgc acgatcagac ttaaacgcca gcgctggaga aaaagcgtgt
     6181 gtaacgctga agtgatcgtg ctgttagcat cgaccaactg tcgggcttgt tctgcccatg
     6241 gggaattcag acgatgcagt aagtctagga tctgtggtag ctgcacaata aactgcggcg
     6301 tggagaggag ctggctttgc tgatagcaca tcacctcttc ggtgagttcc ggcgggactc
     6361 gggcatcttt cagccgactg cgcagcgcct cacgccagcg gggaacatca tcagtgattg
     6421 ccgccttcag tcgtgggaat ttttcaaaga agactgccag ctgcggtgag gccagcagcg
     6481 cgatgatcat ctcttcgatc aatccctctt cgctgacggc cagcatcaca ttaagcgtat
     6541 ccagcgttag cattgttgtg cctggcgaat ctgtgcgccg acatcctgta ggctggcttc
     6601 aatgcgacct aaccagtcgc cagggataaa cagacatctt tgctgttcgc tgaataaagc
     6661 gtgttgcttg cgccagtcgc tttcaagctc ttccaattgt tgttttattt cacctggcag
     6721 accttcagcc gatgaaccgg gaagtgccag cgtactgcct tgtaaactta catcgcgtac
     6781 aacaagatgt tgggcgctat caacttccag attcagtttc tgggcaaagc cgataccgtt
     6841 cagtttgccg cgaatttcac cacctttgct cagccactgt tccagcgcgc tacgctcaaa
     6901 ggagatatga accacttcca tattatgcaa ttttaacggt ttttgcagca gcagagtcag
     6961 agtggaagca gtaacgttaa caggaagttg atactgctga cgacggctga aaatgccgcc
     7021 cagacgaatt accgttaagg ctgttttatc gctttgttgc tgctggagtt gcaggtgacg
     7081 ttgcacaatc gcgcccaggc gggtcaacat cccttgctgt tgccaggcat gaccggtcat
     7141 caatacatca atttgttgtt gtatcaaatt caggctttgc gcgtcatacc acaggcaatc
     7201 tttcagcaaa atgagatcta ccggggcaac agcactgcga ccgctaaaaa aggcgctggc
     7261 ctgcaataat cgaatcgctt ttttccagcg acgatccgag acataaggcg catccggtaa
     7321 tttatccagt tgctggcgca gcataaaaat cagctcaaat acatgatcgg gcagcgtaat
     7381 ttcaccaatc tctttttgcc agcgttcata ttcttcatct gtgacctgca aggaggctgg
     7441 aaccggattg tcattttcat cctgttgact ggtcagcatg gagcggaaat tcgctttatc
     7501 ctgcacttta tctaaccaca gacgaatcag catgcggtca tataacgctt ccagactgct
     7561 gtctgcttcc ggcagctcgt tggaggccgc caccagcagg cgcatcggga ttttttctac
     7621 gtgtgcaccg ttgcggaact ggcgctcgtt aatggcggtg agcagggtat taagaattgc
     7681 cggacccgct ttccagatct catccagaaa gacgatttct gcttccggca ggtaaccgct
     7741 ggttaaacgt tcatagcgcc cttcatcttt tagcgcctga atagaaaggg gaccaaaaac
     7801 ttcttccggc gtggagaagc gggtcatcag atattcaaac gcgcgggcat tctgaaaggc
     7861 gaattttaag cgccgggcga tcaaactttt ggcaatacct ggcgggccaa ggaggaacac
     7921 actttcacca cttaatgccg ctaataaaca caagcggatg gcgtggctac gttcataaag
     7981 ccccttttcc aacgaactgc tcaggcggga aattctttcc gctaataaat gagggtgagc
     8041 cataatgaag tggcgtcctt tcgtcaaaag ttctgcgtaa attgcgagta tagacgtttc
     8101 ttgctggtgg ctaaaatagt ctcaaagggg gggtattttt ctttgagcca ggttaatgtg
     8161 gccgcattta ggagtacgat tttgccgtta atcgtgcata ctgtgcgctt ttttgtgggc
     8221 caagggacta agcacacatt tcatatttca acgaaagact agtctatgag cactgataat
     8281 aagcaatcat tgcccgcgat taccctcgcg gcgattggag ttgtttacgg cgatattggt
     8341 accagcccgt tatatacact tcgtgaatgt ttgtccggcc agtttggttt tggcgttgaa
     8401 cgcgatgccg tgtttggctt tttatcgctg atcttctggc tgctaatctt tgtggtttcc
     8461 attaaatatc tcaccttcgt gatgcgggca gataacgccg gtgaaggggg gatcctgacg
     8521 ttgatgtcgc ttgccgggcg taatacgtcg gcgcgaacca catcaatgct ggtgattatg
     8581 gggctaatcg gcggcagctt tttctatggt gaagtcgtca taacacccgc tatttcggtg
     8641 atgtcagcca ttgaaggtct ggaaatcgtc gccccgcagc tggatacctg gatagttccc
     8701 ctctcaatta tcgttctcac tttattattt atgattcaaa agcatggcac ggctatggtc
     8761 ggtaagctgt ttgcgccgat catgctgacc tggtttttga ttctggcagg actggggtta
     8821 cgtagcatta ttgctaacca ggaagtgctg tatgcgctga atccgatgtg ggcggtgcat
     8881 ttcttccttg aatacaaaac ggtttctttt attgcattag gggcagtggt gctgtcgatt
     8941 acgggggtcg aggcgctgta tgctgatatg gggcactttg gtaagttccc tattcgcctg
     9001 gcgtggttca ccgtcgtatt accttccttg acccttaatt acttcggtca gggagcgctg
     9061 ttgttaaaga atccggaagc gattaagaac ccgttcttcc ttttggcacc ggactgggcg
     9121 ctgatcccgc tgctgatcat cgccgcactg gcgacggtaa tcgcctcgca ggcggttatc
     9181 tctggcgtct tttcattgac gcgtcaggcg gtacgtctgg gatatttgtc gccgatgcgc
     9241 attattcaca cctccgaaat ggagtcaggg caaatctata ttccgtttgt gaactggatg
     9301 ctctatgtcg cggtcgtgat tgtgattgtc agctttgagc actccagcaa cctggcggcg
     9361 gcgtacggga ttgcggtgac cggaaccatg gtgctgacgt ctattctctc gactaccgtg
     9421 gcacgtcaga actggcactg gaataagtat tttgttgcgc tgatcttgat tgctttcctt
     9481 tgtgtcgata ttccattgtt caccgctaac ctcgataaac tgctctccgg cggctggttg
     9541 ccattgagcc tcggtacggt gatgtttatc gtgatgacca cctggaagag tgagcgtttc
     9601 cgcttgctgc ggcggatgca tgaacatggt aactctctgg aagcgatgat tgcttcgctg
     9661 gagaaatcac cgcctgttcg cgtgcccggg accgcggtat atatgtcgcg tgcaatcaac
     9721 gtcattccct ttgcgctgat gcataacctt aaacataaca aggtattgca tgagcgggtg
     9781 attctgttaa ctctgcgcac cgaagacgct ccatatgtcc ataacgtccg tcgggtacag
     9841 attgaacaac tgtcgcccac tttctggcgc gtggtggcaa gttatggttg gcgagaaacg
     9901 ccaaacgtgg aagaagtttt ccaccgctgc ggtctggaag gattaagttg ccggatgatg
     9961 gaaacctcct tctttatgtc gcatgagtcg ttgatcctcg gcaaacgccc gtggtatttg
    10021 cgtctgcgcg gcaagctgta cttgctgctg caacgtaatg cgctgcgtgc accagatcaa
    10081 tttgaaatcc cgccaaacag ggttatcgaa ctgggtactc aggtcgaaat ctaacgccag
    10141 acgcctcctt tcttcataag ggggcgtttt tgttttcatg gttaatcacc atgtaaaacg
    10201 tttcgaggtt gatcacattt ccgtaacgtc acgatggttt tcccaactca gtcaggatta
    10261 aactgcgggt cagcgaaacg tttcgctgat ggagaaaaaa atgaaaaaag gcaccgttct
    10321 taattctgat atttcatcgg tgatctcccg tctgggacat accgatacgc tggtggtgtg
    10381 tgatgctggt ttacccatcc ccaaaagtac aacgcgtatc gatatggcat taacccaggg
    10441 tgtaccttct tttatgcagg tgctgggcgt cgtcacaaat gaaatgcagg tcgaggctgc
    10501 cattatcgcg gaagagatca aacaccataa tccgcaactc cacgaaacgt tgctcactca
    10561 ccttgagcag ctgcaaaaac accagggaaa taccattgaa attcgttaca ccacgcatga
    10621 acaattcaaa caacaaaccg cagaaagtca ggcggtaatt cgcagcggag aatgttctcc
    10681 gtatgcgaat atcattctct gtgctggcgt gacgttctga ggccgtcatg gaagcattac
    10741 ttcagcttaa aggcatcgat aaagccttcc cgggcgtaaa agccctctcg ggcgcagcgt
    10801 taaatgtcta tccgggccgc gtgatggcgc tggtgggcga aaacggcgcg ggtaaatcca
    10861 ccatgatgaa agtgcttact ggcatctata ctcgcgatgc cggtacgctt ttatggctgg
    10921 ggaaagaaac gacatttacc gggccgaaat cttcccagga agccgggatt gggattatcc
    10981 atcaggaact gaacctgatc ccgcagttaa ccattgccga aaacattttc ctcggtcgtg
    11041 agtttgttaa tcgctttggc aaaattgact ggaaaaccat gtatgccgaa gcggataaat
    11101 tgctggctaa acttaacctg cgctttaaaa gcgacaagct ggtgggcgat ctttccatcg
    11161 gtgaccagca aatggttgaa atagccaaag tgctgagctt tgagtcgaaa gtcatcatta
    11221 tggatgaacc gaccgatgcg ctgaccgata ccgaaaccga atccctgttc cgcgtcatcc
    11281 gcgagctgaa atcgcaaggc cgcggtattg tctatatctc ccaccgcatg aaagaaatct
    11341 tcgagagttg cgatgacgtt accgtttttc gtgacggaca atttattgct gagcgcgaag
    11401 tggcatcact gaccgaagat tcgctgattg agatgatggt gggtcgcaag ctggaagatc
    11461 aatatccgca cctggacaaa gcgccgggag atatccgcct gaaagtcgat aatctctgcg
    11521 gacctggcgt taacgatgtc tcttttactt tacgcaaagg cgaaattctt ggcgtctctg
    11581 gtttgatggg cgcaggtcgt accgaactga tgaaagtgct ctacggcgca ctaccgcgca
    11641 ccagcggtta cgtcaccctg gatgggcatg aagtcgttac ccgttcaccg caggatggcc
    11701 tggcaaacgg cattgtgtat atctccgaag accgtaaacg tgacggttta gtgttgggca
    11761 tgtcagtaaa agagaacatg tcgctgacag cgctgcgcta cttcagccgc gctggcggca
    11821 gtttgaagca tgccgatgaa cagcaggctg tgagtgattt tattcgcctg ttcaatgtga
    11881 aaacgccgtc gatggagcag gcaattggtc tgctttccgg tggcaatcag caaaaagtgg
    11941 cgattgcccg cggtctgatg acacgcccca aagtgttgat ccttgatgaa cctacccgtg
    12001 gcgtagatgt cggcgcgaaa aaagagatct atcaactgat taaccagttc aaagccgatg
    12061 gcttgagcat cattctggtg tcatcggaga tgccagaagt attaggcatg agcgatcgca
    12121 tcatcgtcat gcatgaaggg catctcagcg gggaatttac tcgtgagcag gccacccagg
    12181 aagtgttaat ggctgccgct gtgggcaagc ttaatcgcgt gaatcaggag taaaaaaatg
    12241 acaacccaga ctgtctctgg tcgccgttat ttcacgaaag cgtggctgat ggagcagaaa
    12301 tcgcttatcg ctctgctggt gctgatcgcg attgtctcga cgttaagccc gaactttttc
    12361 accatcaata acttattcaa tattctccag caaacctcag tgaacgccat tatggcggtc
    12421 gggatgacgc tggtgatcct gacgtcgggc atcgacttgt cggtaggttc tctgttggcg
    12481 ctaaccggcg cagttgctgc atctatcgtc ggcattgaag tcaatgcgct ggtggctgtc
    12541 gctgccgctc tcgcgttagg tgccgcaatt ggtgcggtaa ccggagtgat tgtagcgaaa
    12601 ggtcgcgtcc aggcgtttat cgctacgctg gttatgatgc ttttactgcg cggcgtgacc
    12661 atggtttata ccaacggtag cccagtgaat accggcttta ctgagaacgc cgatctgttt
    12721 ggctggtttg gtattggtcg tccgctgggc gtaccgacgc cagtctggat catggggatt
    12781 gtcttcctcg cggcctggta catgctgcat cacacgcgtc tggggcgtta catctacgcg
    12841 ctgggcggca acgaagcggc aacgcgtctt tctggtatca acgtcaataa aatcaaaatc
    12901 atcgtctatt ctctttgtgg tctgctggca tcactggccg ggatcattga agtggcgcgt
    12961 ctctcctccg cacaacccac ggcggggact ggctatgagc tggatgctat tgctgcggtg
    13021 gttctgggcg gtacgagtct ggcgggcgga aaaggtcgca ttgttgggac gttgatcggc
    13081 gcattaattc ttggcttcct taataatgga ttgaatttgt taggtgtttc ctcctattac
    13141 cagatgatcg tcaaagcggt ggtgattttg ctggcggtgc tggtagacaa caaaaagcag
    13201 taataacgac tacaggacat cttgaatatg aacatgaaaa aactggctac cctggtttcc
    13261 gctgttgcgc taagcgccac cgtcagtgcg aatgcgatgg caaaagacac catcgcgctg
    13321 gtggtctcca cgcttaacaa cccgttcttt gtatcgctga aagatggcgc gcagaaagag
    13381 gcggataaac ttggctataa cctggtggtg ctggactccc agaacaaccc ggcgaaagag
    13441 ctggcgaacg tgcaggactt aaccgttcgc ggcaccaaaa ttctgctgat taacccgacc
    13501 gactccgacg cagtaggtaa tgctgtgaag atggctaacc aggcgaacat cccggttatc
    13561 actcttgacc gccaggcaac gaaaggtgaa gtggtgagcc acattgcttc tgataacgta
    13621 ctgggtggca aaatcgccgg tgattacatc gcgaagaaag cgggtgaagg tgccaaagtt
    13681 atcgagctgc aaggcattgc tggtacatcc gcagcccgtg aacgtggcga aggcttccag
    13741 caggccgttg ctgctcacaa gtttaatgtt cttgccagcc agccagcaga ttttgatcgc
    13801 accaaaggtt tgaacgtaat gcagaacctg ttgaccgctc atccggatgt tcaggctgta
    13861 ttcgcgcaga atgatgaaat ggcgctgggc gcgctgcgcg cactgcaaac tgccggtaaa
    13921 tcggatgtga tggtcgtcgg atttgacggt acaccggatg gcgaaaaagc ggtgaatgat
    13981 ggcaaactag cagcgactat cgctcagcta cccgatcaga ttggcgcgaa aggcgttgaa
    14041 accgcagata aagtgctgaa aggcgagaaa gttcaggcta agtatccggt tgatctgaaa
    14101 ctggttgtta agcagtagtt ttaatcaggt tgtatgacct gatggtgaca taaatacgtc
    14161 atcgacagat gaacgtgtaa tataaagaaa agcagggcac gcgccaccct aacacggtgg
    14221 cgcattttat ggacatcccg aatatgcaaa acgcaggcag cctcgttgtt cttggcagca
    14281 ttaatgctga ccacattctt aatcttcaat cttttcctac tccaggcgaa accgtaaccg
    14341 gtaaccacta tcaggttgca tttggcggca aaggcgcgaa tcaggctgtg gctgctgggc
    14401 gtagcggtgc gaatatcgcg tttattgcct gtactggcga tgacagcatt ggcgagagcg
    14461 ttcgccagca gctcgccact gataacatcg atattactcc ggtcagcgtg atcaaaggcg
    14521 aatcaacagg tgtggcgctg atttttgtta atggcgaagg tgagaatgtc atcggtattc
    14581 atgccggcgc taatgctgcc ctttccccgg cgctggtgga agcgcaacgt gagcgtattg
    14641 ccaacgcgtc agcattatta atgcagctgg aatcaccact cgaaagtgtg atggcagcgg
    14701 cgaaaatcgc ccatcaaaat aagactatcg ttgcgcttaa cccggctccg gctcgcgaac
    14761 ttcctgacga actgctggcg ctggtggaca ttattacgcc aaacgaaacg gaagcagaaa
    14821 agctcaccgg tattcgtgtt gaaaatgatg aagatgcagc gaaggcggcg caggtactgc
    14881 atgaaaaagg tatccgtact gtactgatta ctttaggaag tcgtggtgta tgggctagcg
    14941 tgaatggtga aggtcagcgc gttcctggat tccgagtgca ggctgtcgat accattgctg
    15001 ccggagatac ctttaacggc gcgttaatca cggcgttgct ggaagaaaaa ccattgccag
    15061 aggcgattcg gtttgcccat gctgccgctg cgattgccgt aacacgtaaa ggcgcacaac
    15121 cttccgtacc gtggcgtgaa gagatcgacg catttttaga caggcagagg tgacgcttgg
    15181 ctacaatgaa agatgttgcc cgcctggcgg gcgtttctac ctcaacagtt tctcacgtta
    15241 tcaataaaga tcgcttcgtc agtgaagcga ttaccgccaa agttgaagcg gcgattaaag
    15301 aactcaatta cgcgccatca gctctggcgc gtagcctcaa actcaatcaa acacatacca
    15361 ttggcatgtt gatcactgcc agtaccaatc ctttctattc agaactggtg cgtggcgttg
    15421 aacgcagctg cttcgaacgc ggttatagtc tcgtcctttg caataccgaa ggcgatgaac
    15481 agcggatgaa tcgcaatctg gaaacgctga tgcaaaaacg cgttgatggc ttgctgttac
    15541 tgtgcaccga aacgcatcaa ccttcgcgtg aaatcatgca acgttatccg acagtgccta
    15601 ctgtgatgat ggactgggct ccgttcgatg gcgacagcga tcttattcag gataactcgt
    15661 tgctgggcgg agacttagca acgcaatatc tgatcgataa aggtcatacc cgtatcgcct
    15721 gtattaccgg cccgctggat aaaactccgg cgcgcctgcg gttggaaggt tatcgggcgg
    15781 cgatgaaacg tgcgggtctc aacattcctg atggctatga agtcactggt gattttgaat
    15841 ttaacggcgg gtttgacgct atgcgccaac tgctatcaca tccgctgcgt cctcaggccg
    15901 tctttaccgg aaatgacgct atggctgttg gcgtttacca ggcgttatat caggcagagt
    15961 tacaggttcc gcaggatatc gcggtgattg gctatgacga tatcgaactg gcaagcttta
    16021 tgacgccacc attaaccacg atccatcaac caaaagatga actgggggag ctggcgattg
    16081 atgtactcat ccatcggata acccagccga cccttcagca acaacgatta caacttactc
    16141 cgattctgat ggaacgtggt tcggcttaga tttacgctgt cttttgatca aattattacc
    16201 atcggttgtt ttcagaagca tgaacattgc tgctgaagca acagtaataa tgcccattgt
    16261 gataaacgta tagtggaatt gttcgacagt cgttgtgcct tccattcctt cataaacgcg
    16321 aaggacggcc gcacttacag ctacgcctaa actgatagac agttgctgcg tgaccgccag
    16381 aacactgtta ccgctgctgg cgttgtcatc ggtcagatcg gcaagtgtga tggtattcat
    16441 cgcggtaaac tgcgtcgaca tagccatccc taatataaac aacggcaaga tcagcatcca
    16501 tattgccatt gccggtgatt gcaaagagaa ctgagcgatc attagcccaa taatcaccgt
    16561 gatccccact aatgtatggc gatagcccag acgacgtaag acttgggtaa ccatcgattt
    16621 tgcaataatg gaacctaacg ctgtcggtgc catcatacag ccagcaataa acgcctgata
    16681 accaaatcct acctgtaaca ttaatggcat aaggaacggt acacagccgg tccccagacg
    16741 ggttgcaata ttgcctacga taccgatcga gaaagtgcgg gttttaaata aatctaatga
    16801 aattaatggg tttggcgtgc gtcgtgcatg gagaatatag agaagcagta acccgatgct
    16861 ggtgacaatt accgtcaagg caatccagct ggcgacaatc ttttccccga atagctctat
    16921 tccgcttgag aagagaacaa ggctgaggcc aaacagcaaa aagccagtga tatcgaatct
    16981 gcgtcgtgcg gtggtgaaat tgggcatatg tttgcgcgcg taaagaaggc ccgcaatacc
    17041 tatggggata ttgattaaaa atatccagtg ccaggttgcc caggtgacca gcacgccgcc
    17101 aagaatgggg cctaaaattg gccccaccag acccggcatg gcgacaaaat tcaatactgg
    17161 aagaagttca ttacgaggat aagcgcgcag taaggccagc cgagcaacag gcatcatcat
    17221 tgcgccgcct atcccctgaa taacccggaa gacaaccagc tgtggtagcg aattagaaag
    17281 tgcgcaggcc agagaaccca atgtgaacag actcacggca agggtaaaaa tgcgacgcgt
    17341 accgaagcga tcggctagcc atccgcttac cggaataagc atcgccaccg tcagcgtata
    17401 actgatgatg gctgattgca tcgcgagagg agaacgatta aggctatgag cgattgcggg
    17461 taaggcggta ttcagaatag tggcatcaag tgcctgcatg aagaaggcca tcgccgcgat
    17521 ccacggcaaa cccgccatac tgcgcttctt tttatcgctc attcaatgtc ctgttatcgg
    17581 gttatcactt atcaggtgag cgtagcagcg cctgacaagc tttaaatgcc gcgtcgccat
    17641 cgctttggat aatcgcatcg actatcgcct gatgcagatc cagctttatc actgtgtcgc
    17701 tggtaattga cgtgaagtaa gtgtgataaa ccgaatggaa tagcgaggcg aatgaggtca
    17761 aaaacggatt ggcgctcatt tcatagatat gctcatgcca ggccatatcg acttcgatcc
    17821 agcgttcacg gcgaaagttc tcttttaatg ccgccatttc ggccattaac gtattgagat
    17881 gcgctttctg ttccgcggtg ccaaccgttg ctgccagtag gcaagcttgt ggctccagac
    17941 agatacgcat aaccagaaag tgatcgatga cctgatgaaa gttctcttct gtcatccacc
    18001 aggtaagcaa ttcctgatca agaaaattcc agttcgattg tggcatgacc cgggtaccaa
    18061 ttcgcggtcg cggtaaaacc atcccttttg ccgttaacgt tttgaccgct tcgcgtaccg
    18121 ctgtacgact cactccaaat tgctcgccca gctcaatctc accgggcaaa atggtgccgg
    18181 gttcatattc accttttaag atccgttgcg ccagcttctc agccagaaca tacgaaaggt
    18241 ttttctgtgc agctaactgt tgtgcgctta aaggcattac taatcttcct ttttcttttt
    18301 attcctcctt agtatgccac caggaagtgt gattacggtt gcaaaaacgg caaattgctt
    18361 gttttatggc acattaacgg ggcttttgct gaaaaaatgc gcggtcagaa aattatttta
    18421 aatttcctct tgtcagaccg gaataactcc ctataatgcg ccaccactga cacggaacaa
    18481 cggcaagcac gccgccgggt cagcggggtt ctcctgagaa ttccggcaga gaaagcaaaa
    18541 ataaatgctt gactctgtag cgggaaagcg tattatgcac accccgcgcc gctgagaaaa
    18601 agcgaagcgg
//
LOCUS       DAZKIP010000084        18420 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZKIP010000084 DAZKIP010000000
VERSION     DAZKIP010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.084"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            35..652
                     /locus_tag="ACHOUK_003205"
                     /pseudo
     CDS             35..652
                     /locus_tag="ACHOUK_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            755..1258
                     /gene="yfbM"
                     /locus_tag="ACHOUK_003206"
     CDS             755..1258
                     /gene="yfbM"
                     /locus_tag="ACHOUK_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFP9898598.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPVKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKKFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1325..2782)
                     /gene="nuoN"
                     /locus_tag="ACHOUK_003207"
     CDS             complement(1325..2782)
                     /gene="nuoN"
                     /locus_tag="ACHOUK_003207"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFP9898599.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2789..4318)
                     /gene="nuoM"
                     /locus_tag="ACHOUK_003208"
     CDS             complement(2789..4318)
                     /gene="nuoM"
                     /locus_tag="ACHOUK_003208"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFP9898600.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4549..6390)
                     /gene="nuoL"
                     /locus_tag="ACHOUK_003209"
     CDS             complement(4549..6390)
                     /gene="nuoL"
                     /locus_tag="ACHOUK_003209"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFP9898601.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6387..6689)
                     /gene="nuoK"
                     /locus_tag="ACHOUK_003210"
     CDS             complement(6387..6689)
                     /gene="nuoK"
                     /locus_tag="ACHOUK_003210"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFP9898602.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6686..7240)
                     /gene="nuoJ"
                     /locus_tag="ACHOUK_003211"
     CDS             complement(6686..7240)
                     /gene="nuoJ"
                     /locus_tag="ACHOUK_003211"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFP9898603.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7252..7794)
                     /gene="nuoI"
                     /locus_tag="ACHOUK_003212"
     CDS             complement(7252..7794)
                     /gene="nuoI"
                     /locus_tag="ACHOUK_003212"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFP9898604.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7809..8786)
                     /gene="nuoH"
                     /locus_tag="ACHOUK_003213"
     CDS             complement(7809..8786)
                     /gene="nuoH"
                     /locus_tag="ACHOUK_003213"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFP9898605.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8783..11509)
                     /gene="nuoG"
                     /locus_tag="ACHOUK_003214"
     CDS             complement(8783..11509)
                     /gene="nuoG"
                     /locus_tag="ACHOUK_003214"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFP9898606.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSLE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQCRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11562..12899)
                     /gene="nuoF"
                     /locus_tag="ACHOUK_003215"
     CDS             complement(11562..12899)
                     /gene="nuoF"
                     /locus_tag="ACHOUK_003215"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFP9898607.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12896..13396)
                     /gene="nuoE"
                     /locus_tag="ACHOUK_003216"
     CDS             complement(12896..13396)
                     /gene="nuoE"
                     /locus_tag="ACHOUK_003216"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFP9898608.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHANLTPEAIPE
                     LLERYK"
     gene            complement(13399..15201)
                     /gene="nuoC"
                     /locus_tag="ACHOUK_003217"
     CDS             complement(13399..15201)
                     /gene="nuoC"
                     /locus_tag="ACHOUK_003217"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311197.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFP9898609.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPARVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15295..15957)
                     /gene="nuoB"
                     /locus_tag="ACHOUK_003218"
     CDS             complement(15295..15957)
                     /gene="nuoB"
                     /locus_tag="ACHOUK_003218"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFP9898610.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15973..16416)
                     /gene="nuoA"
                     /locus_tag="ACHOUK_003219"
     CDS             complement(15973..16416)
                     /gene="nuoA"
                     /locus_tag="ACHOUK_003219"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFP9898611.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17047..17985)
                     /gene="lrhA"
                     /locus_tag="ACHOUK_003220"
     CDS             complement(17047..17985)
                     /gene="lrhA"
                     /locus_tag="ACHOUK_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFP9898612.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPISAPEGDDSLLIERDIE"
BASE COUNT         4593 a         5124 c         4734 g         3969 t
ORIGIN      
        1 ataatttaca tatgaaaaaa attttccttt tgttatatta tttctgtttt cactccctct
       61 gattattttt taccaaccct gggtcactgc attatcgcca acgccccgac acgtaattcc
      121 tgagcaatta gaaaaaacag ttcgttatct tacacaaact gtgcatccac gtaatgccga
      181 caatattgat aatctgaata ggtccgtaga gtatataaaa aaaggtcttt gtcagtagcg
      241 gtgccagagt taccgcgcag ggcgtcccca attggctatt acgactctgc gcctggtagc
      301 caggattacc cttacccgca atgtcctggc tttatcccga tcggggggac tttattgcct
      361 ggtcggcaga atacaggata tcaacgccgt tcgtcaggta aaaacggcat tgttatcatc
      421 ccgggattta tctgtttatt ctatgaatgc cccatggttt attcacggta ttgatttctc
      481 tgaccacctt aattattggc aacacgatat tctcgccgta atgattactg acaccgcttt
      541 ttatcgtaat aaacaatacc acttgcccgg tgatacagca gacagattga attatcagaa
      601 aatggctcag atggtggatg gtgttataac tttgttacac aacagtaaat aataaaaatt
      661 tctcgaggcg ggtccagaaa cagcgacatg tacattttat tttccctcgt ctgttttaag
      721 atgcgaaaca taaaattcaa tggaggaaaa ataaatggga atgattggtt attttgcaga
      781 aattgactca gaaaagatta accaactgct tgagtctact gaaaaacctt tgatggataa
      841 tatacatgac acgctttcag gattacgccg actggatatt gataaacgct gggatttttt
      901 acactttggg ctgacaggca cctctgcgtt cgatccagtg aagaatgatc cgctgagtcg
      961 tgccgtgctt ggtgaacaca gcctcgaaga tggtatagat ggtttcctgg gattgacatg
     1021 gaatcaggag ttagccgcga caatcgaccg actggaatca ctggatcgaa gtgagctgcg
     1081 taaaaagttt tccattaagc gactcaatga aatggagatt tatccgggag tgaccttttc
     1141 agaagagtta gaggggcaac tctttgcttc gatcatgctt gatatggaaa agctgattag
     1201 cgcgtatcga cgtatgctgc gacaagggaa tcatgcgctg acggttattg ttgggtgatg
     1261 gtgtcagggc accaaaaaaa accgccgaac atgtcggcgg ctttctgact tacaaactga
     1321 tagcttacat cagcggcatt gctaaccgca caatactaat cagcggttgt ggccatacac
     1381 ccagcaccag taccaacagt gcagaaatca gcaccacgat accgcccgcg ctgtactgcc
     1441 agtttgacgg tgcatcgcga cccggttgtt ccggtgcgtg caggtacagg ctcaccgcca
     1501 cgcgcaggta gtagtagagg ccgattgccg aaccgacaac cacggcaccc accagccacc
     1561 acaggtgtgc ctggacacca accgccagca cgtagaactt accgataaag cccagcgtca
     1621 tcgggatacc ggccagagac agcatcatca ccgtcatcac cgccgcgagg atcggacgat
     1681 gccagaacag accgcggtaa gagaacaggg aatcagcatc cgggccacga tacgggctgg
     1741 acatcaggct gaccacgccg aacgcgccga ggctgctgaa cagataaccg gccaggtaaa
     1801 cccctaccgc ttccatcgac atctcgccag tttgcagcgc aatcagcgcc accagcagat
     1861 agccgaggtg agagatagat gagtaaccga gcagacgttt gatattggtc tggctcagcg
     1921 ccatcaggtt accgaagatg atggaggcaa aggcgataat cgccagcacc acccgaatcg
     1981 cttcgctgtc acccaccggt gcgtagagga acagacgcat caccacaccg aagatagcga
     2041 ttttgctcgc cgtcgccagg aaagtggaaa ccggcgcagg cgcgccctgg tatacgtctg
     2101 gcgtccacag gtggaacggc accagagaga gtttgaagcc gaggccaaca atcatcaggc
     2161 cgaaacctgc cagcaacagc ggctcgttta gcataccgtc gccgaggttt ttacccagcg
     2221 cgacaaacga caggtcgcca gactgcgcat acaccagcgc cataccgaac agcaggaaag
     2281 aagacgctgc ggcagaaagg atggtgtatt tgatactggc ttccagcgaa cgtttctggc
     2341 ggaaagcgta accgaccagg ccaaacagcg gcaaagagat cagttcaata ccgaggaaca
     2401 gagacgccag atggtttgca ttcgccagca ggatcccgcc cagcgcggca attaacacca
     2461 acaggtagaa ctcatccttg ttgtcgttat agccttcaag ccacgggtag gcgaaagtac
     2521 aggtggcgag gctcgccaac aataccagcc cggtgtaaag catggcgaaa ccatcaacgc
     2581 gcatcagcgg cgtaacgtcc atagcgcccg cctggccaac aaaccagagc gaaaccagcg
     2641 ccgcgttaag cccaataacc gagagcgtag cgttgaggaa atgattgcgt cgccacgcaa
     2701 tggagagcat cacaaccacc accgtcaagc cgacgatcag caacggtagc agtgcgatca
     2761 ggttttgtgg agttattgtc atggcgattt acggccttgt agtagtaacg gaattaacaa
     2821 accactgctg gatattgcca atcgcggagt gcgaggtatc cagaatcggc tgcggataga
     2881 agcccagcag taccagcagc accaccagca acaggatcat aaacagctca cgcagcgaca
     2941 tccctggcag ttcctggctg gcaatctggc ttttcgcttt accgaagtaa gcgcgatgta
     3001 acatcgccag cgaataaaca gatgcaaaga ccagcccaaa ggtagagata acggtaatca
     3061 ccgggacaac ctggaagctg ccgaacagaa tcataaattc gccgacgaag ttaccggtgc
     3121 ctggcatccc gagcgttgcc accgcaaaga acagtgacaa ggctggcagc catttcatct
     3181 tgctccacag accgcccatc atgcgcatgt cgcgggtatg gatacgttca taaagctgac
     3241 cacaaagaat aaacagaccc gccgctgaca agccgtgcgc aatcatctgg attaccgcgc
     3301 cctggtaggc caactggctg ccggtgtaga tagcaatcag tacgaagccc atgtgggaaa
     3361 ccgaggtgta ggcgatcaga cgtttgatat cggtctgggc gaaggccatc cacgcaccgt
     3421 agaagatgcc gataacaccc agccacatgg cgattggcgc gaactctgcc gacgcgtttg
     3481 ggaacagcgg tagggagaaa cgcagcagac cgtaagcggc ggttttcagc aagatccccg
     3541 cgaggtccac ggaaccggcg gtcggtgcct gtgagtgcgc atccggcagc cagccatgca
     3601 gcggaaccac cggcattttc accgcaaacg cgatgaagaa gcccagcatc aacagatatt
     3661 ccacaccact ggacattggc gtattcagca gctcttcata gttgaaggtc cagacgccgg
     3721 tcgcattgta gtgaacaaaa accagcgcca ggatggcgat caacatcacc agaccactcg
     3781 cctgggtgta aatgaagaac ttggttgccg ccgtgatacg cgttttacca tcagaggctt
     3841 tatgccccca cagtgcgatc aggaagtaca tcggcaccag catcatttcc cagaagaaga
     3901 agaacaggaa catgtcgatg gcaaggaaca cgccgataac gccgcccagg atccacatca
     3961 ggttgaggtg gaagaagccc tgatattttt cgatctcttt ccacgaacag agtaccgcca
     4021 gcacaccgag cagaccggtc agcacgacca tcagcagcga cagcccgtca atggcgagat
     4081 ggatagagat accaaaacgc gggatccacg gcatgtcgaa ttcagactgc cactgcggaa
     4141 ttccggcgga ttgcgtcagt gaataaccgc cctgcaacca cagttgcagc gacagcgcca
     4201 gcgtcaatcc catggtgatc agcgcgatcc agcgcggcac cttgacgcca aagcgttcgg
     4261 tctgccagca caggaagccg ccgataaagg ggattagtat taaccagggt aataacatag
     4321 ctaagtatgt cccttatatt tactcctgct caggctatct cactcgccag cccgaatccc
     4381 ggcagtgctc gcaatcctca cgtacttaag tacgctccgg ttgctgtgcg ctggcggtat
     4441 ccggtctgac ttcgccgatg acgcctgtac ttcaggaaca gttacctaaa atttattctt
     4501 agttcacaga cccggcgcaa cgccgggtat attcactcag ccaacaactc aacgcagtac
     4561 catcaacagt gccagcacca cgaccgcacc gatgctcatg gatgccacat accagcgcag
     4621 ataaccgttc tcgcttaaca gcaggccttt acctgcaaag cgggaaagga cagccgggat
     4681 gttcatcatt gagttcagcg gatcgcgttt cagcaaccag gcaataccca ggaacggctt
     4741 gacgaacact ttgtcgtaca gccagtcaaa gccccaggcg ttgtaccacc aggtgcccag
     4801 cagacggccc ggcgcactgt tggcgatgga ggtcaccaga gtacgtttac ccagccacag
     4861 ccaggctgcc agcagaatgc cgaccaccgc gaccacgcca gaggtaattt ccagggtcaa
     4921 catgctgccg tgcgccagtt ccgtcgtttg cggaagcacg ccctgcagcg gcggtacaat
     4981 cagtgcgcca acgaaggtgg aaaggatcag cagcacaatc agcggcaggc tgtgagttac
     5041 ccctttcacg gcgtgagcgt gaatttgttc ttttccgtgg aagacgatga aaatcatacg
     5101 gaaggtgtag agcgaggtca taaacgcacc gaccagacct gccaccatca gattgatatg
     5161 accattcgcc atcgcacccg cgaggatctc atccttactg aagaagcccg cagtgactag
     5221 cggcagtgcc gacagtgctg cgccgcccac caggaagcag agataaacca gcggaataga
     5281 tttacgcaga ccgcccatct tgaagatgtt ctgttcgtga tggcaggcga gaatgacgga
     5341 accggatgcc aggaacagca gcgctttaaa gaacgcgtgg gtcatcaagt gaaaaatcgc
     5401 cgcatcccat gcctgcacgc caagcgcgag gaacatgtag ccaatctggc tcatggtaga
     5461 gtaagcgaga acacgtttga tgtcggtctg taccagcgcg gcaaaaccgg ccagcagcag
     5521 cgtaaccgcc ccaacaatgc ccaccagatg cagaacttcc ggcgtcatca ggaacaggcc
     5581 gtgggtacgg gcgatcaggt agacacccgc ggttaccatc gttgcggcgt ggatcagcgc
     5641 ggagacaggc gtcgggcccg ccatcgcgtc ggcaagccat gtctgcaacg gcaactgcgc
     5701 agatttaccg accgcaccgc ccagcagcat cagcgtcgcc cacatcagca tgttattgcc
     5761 gtcagcaaag tgcgctggtg ccagttccac catttcgcgg aagttcaggg tgcccagttc
     5821 gttgtaaaga atgaacagcg cgaaagcgag gaacacgtca cccacacggg tcacgacgaa
     5881 cgctttcatt gccgctgcgc cattcttcgg atcggtgtaa tagaacccga tcagcagata
     5941 ggagcacagg cccacgcctt cccagccgag gtacatcagc agcaggttgt cggcaagcac
     6001 cagaaccacc atgctggcga taaacaggtt ggtgtaagcg aagaagcgag agtagccctc
     6061 ttcaccgcgc atataccagg aggcgtacat gtgaataagg aaacccacac cagtgaccac
     6121 cgagagcatg gtcagcgaca ggccgtccag caccaggtta aaaccgatgt taaagtcgcc
     6181 taccgacatc cacgtccaca gcggctggct gtatgcctgc tcgccgttag cgaagaagtc
     6241 aacgccaata aaggcagtta ccagcgccgc caggcccaca gagccaacgc cgacgatcgc
     6301 cgagacgttt tcagaccagc gcccacggga gaacgccagc aggacgaagc caatcaatgg
     6361 caaaataatg gttaaggcaa gcatgttcat ccgcgcatct cacttactga atcgatgttc
     6421 aggttctggc gacgacggtg aagttgcagc agcagcgcaa ggccgatact cgcttctgcc
     6481 gccgcgaggc tgatggcgag aatgtacatc acctgaccgt cggtctggcc ccagtaactt
     6541 ccggcgacca caaaggccag cgcggaagcg ttaatcatga tttccagacc aatcagcata
     6601 aacagcagat tgcgacggat aaccagaccg gttaagccaa gaacgaataa gattgccgcg
     6661 aggatcagtc catgttgtaa ggggatcatg cgtgctcctc cgtttttctt ttcgcgctgt
     6721 cgtctttacg attgctcagc acttcacccg cacgctcttc acgaccgacg tggaaggcca
     6781 caaccagacc cgcgagcagc agcatagaag ccagttccac cgccagtacg taaggcccga
     6841 acagcgtaat acccactgct ttagcactga ttggcgtacc gtcgatacct tgatcgttaa
     6901 caccgagaat ggcgtaaaca atcaccacca acatgatggc tgacaaaatt gccggaccaa
     6961 tccacacctg cggtttcagc cactggcgtt cttgttcgat ttctgaaccg cccaggttga
     7021 gcatcatcac cacgaacacg aacagcacca taatggcacc cgcgtagacg ataatttcca
     7081 gcgcaccggc gaagtaagcg cctagtgaga agaacacccc ggaaatcgcc agcagcgaaa
     7141 taatcaggta cagcagtgcg tgtaccggat tggtatgggt gatcactcgc aaggtcgcaa
     7201 gtatggctat caggccacag atataaaaag cgaactccat tgcccctctc cttacggtaa
     7261 caggctcttg acgtcgatag gcttggcttc gttctctgct tcgcccttat ctttgccgtc
     7321 gattgccata cctgccatcc ggtagaagtt atactccggg tatttgcccg gaccggagat
     7381 cagcagatcc tctttctcgt aaaccagatc ctggcgcttg tattccccca tttcgaaatc
     7441 cggggttaac tgaatcgccg tggtcggaca ggcttcttcg cacaaaccac agaaaatgca
     7501 gcgtgagaag ttgatgcgga aaaattccgg ataccagcga ccgtctttgg tttctgcttt
     7561 ttgcagcgag atacagccga ccgggcaggc taccgcgcag aggttacagg ctacgcaacg
     7621 ctcttcgccg tccgggtcgc gggtcagaac gatacgacca cgataacggg gcggcagata
     7681 gaccggctct tccgggtaca ttcgcgtttc gcgtttggcg aacgcgtgca ggccgatcat
     7741 ccagatacta cgaacctggg tgccgaaacc tactaacaat tcttttaagg tcatggtctt
     7801 attgcccctt attgcgcctg ccagagaatg acagccgccg ttaccagcaa gttgatcagc
     7861 gtcagcggca ggcagatttt ccagccgaag gacattacct ggtcataacg cggacgcggt
     7921 aacgacgcac gaatcaaaat gaacatcatc ataaagaacg cggttttcag cgcgaaccag
     7981 atgaatggcg gtaacaacgg gccttgccag ccaccgaaga acagcgtcac catcaatgca
     8041 gagatggtca caatcccgat gtactcaccc acgaagaaca gaccgaactt cataccggaa
     8101 tattcaatgt ggtaaccatc cgccagttcc tgctcggctt ccggctggtc aaacgggtga
     8161 cggtgacaca ccgccacgcc cgcgatggca aaggtaataa aaccaaagaa ttgcgggatg
     8221 acgttccaca catgcgcctg gctgttgacg atgtcggtca tgttgaatga accggcctgc
     8281 gccaccacgc ccatcaggga aagcccgagg aacacttcgt agctcagggt ctgcgcagaa
     8341 gcacgcatcg caccgagcag cgagtatttg ttgttacttg accagcccgc aaacagcacc
     8401 gcgtaaaccg ccagaccggc catcatcagg aagaacaaaa tcccgatgtt caggtcggca
     8461 accacccaac ccggactgac tggcacaatc gcaaaggcca gcagcagcga ggtaaaggca
     8521 atcatcggtg ccagggtaaa gatgacgcga tccgagaatt tcgggatcca gtcttcttta
     8581 aagaacattt tgatcatgtc cgcaaccagc tggagcgaac caccccagcc aacacggtta
     8641 ggtccgtaac ggttctggaa cagacccagc aggcgacgtt cgccaaagct catgaatgcc
     8701 ccgcaggtga caaccaccag caggatcacc accgctttga ggatggtcag caggatctca
     8761 atcagttccg gtgatatcca actcattgtt gtgcctcctt gagatcctca agatgcgcgc
     8821 cagccagcac cggagcaatg ccggacatac ccatcggcaa gcccacctgc cctgccgtca
     8881 gcccttcagc gatttcaacc ggcagagtga ccgtgttgcc atcgtaacta aaggagacgc
     8941 gtgtacctgc gtttacaccc aacttcgcgg catccgctgg gttgagtttg atgtacggct
     9001 gcggcatacg acactggaag accggagcac gctgtgacaa ttcatcgctg ccaaacaggt
     9061 ggtagtacgg cgcgatacgc catttcccgt cctgcggctg gaagcgtgcc ggtacgctgg
     9121 tgaagtaatc cagaccattt tcgctggttt caaacagacg cacacccgga tcgccaaagc
     9181 gcagtttgcc gcccacttcg tcctggaatt tgttccacgc ctgcggggag ttccagcccg
     9241 gagcccaggc aaacggcact tgcgaacggt gcgcggtcgg ctggttgtta ccttccatcg
     9301 agaaggtgaa catggtgtca atatcctgcg gctgacgcgg ctcatgaacg ctgatattgg
     9361 cgcgcatggc ggtacgaccg ctgtaacggt gcggttcacg ggccagtttc tgcccacgaa
     9421 tacggaatgt cgcatctggc gcagcatctt tgatacccgc cagttccggg attttcgcca
     9481 caacagcgtc aattacatgg tcgagctgcg tccagtccac ttcacggctc agcaaggtgc
     9541 tgtgcagcga gtgcagccag cgccaacttt ccagcatgac agttttgctg tcgtaataag
     9601 ccggatcgta aacctggaag aaacgttggg cgcgaccttc attattaatt accgtaccgt
     9661 cgctttcagc aaagctggca gcggaaagca ccagatgggc gttttccatg atcgctgtgc
     9721 gttgatggtc aaccaccatc accagcggcg ctttagccag cgcagcattc acgcgggtag
     9781 cagaagcgtg acgatgcaga tcgttttcca gcaccaccac cgcgtcggcg cgtccggttt
     9841 ccagttcggt taacgcttct tcaagcgatc caccgcccat aatgcccaac cccatgctgt
     9901 tgacggaacg ggcaatcatg gtgataccga cgtcagcgcc gcgacctttc agggctttcg
     9961 cgacgttagc cgcagcctga atcacctcta agctaccggc gttcgtcccg gagataatca
    10021 acggtttctt cgcacccgcc agtgcctgca cgatgacgtc gattttgctt tgcagctcag
    10081 gttcaatacc gtcaaccgct ggcgcagagt tatccagcgc atgggcgatg gcaaaaccta
    10141 aacgcgcctg atcttcaacc ggtgcgcggt aagtccacgc cgcgatatca tccagacggg
    10201 tgtcatcaac gttggtaaca aacagcggat gcttagcacg ttgaccgata ttgaggattg
    10261 ccgcaatctg ccagtcagcc actttctgtg ctgccgccat ttcgcgcgct ttacctttca
    10321 ccgcctggcg cactgccagc gcgacgcgcg cgccggtctg ggtaacgtct tcgcccagca
    10381 ccagtaccgc atcgtaagat tcgatttcgc gcagagccgg agtataaatg ccgccttcac
    10441 gcagcacttt cagcgccagt tgcagacgtt cctgctcacc gtgagcgata ccggtgtaga
    10501 agttttcttc gcccaccagt tcacgcagcg caaagttgct ttccacgctg gcacgcggag
    10561 aaccaatacc gatcactttc ttcgactgac gcagaatatc tgccgcgccc tgcattgcct
    10621 gttcggcgtt gagggtaatg aaatcatcgc cacgacgctg tactggctga cgcggacgat
    10681 ctttcaggtt gacgtaaccg taaccgaaac gaccacggtc gcagaggaag tagtggttta
    10741 ccgtaccgtt gtaacggttt tcgatacgac gcagttcgcc ataacgctcg ccggggctga
    10801 tgttacagcc gatggaacat tgctggcaga tgctcggcgc aaactgcata tcccatttac
    10861 ggttgtaacg ctcggagtgc gttttgtcgg taaatacgcc ggtcgggcaa atttcgacca
    10921 ggttaccgga aaattcgctt tccagcgtgc cgtcttccgg gcgaccgaag tagacgttgt
    10981 catgcgcgcc ataaacgccc agatctgtac cgtcagcgta atctttgtag taacgcacgc
    11041 agcggtagca ggcgatgcag cggttcattt cgtgagagat gaacggcccc aaatcctgat
    11101 tacggtgggt acgtttggtg aaacggtagc gacggaagct gtgtccggtc atcacggtca
    11161 tatcctgaag atggcagtta ccgccttctt cacataccgg acagtcgtgc gggtggttgg
    11221 tcattaacca ctcgaccaca ctttcacgga actgtttcgc ttcttcgtcg tcaatggaaa
    11281 taaaggtgcc atcggaagcc ggtgtcatac aggacatcac caggcgacca cgcgtgtctt
    11341 ccgcgttttg gtattgcttc accgcacact ggcggcaagc accgacgctt cccagcgccg
    11401 gatgccagca aaagtaagga atatcaaggc ccagagacag acaagcttcc agcaggttgt
    11461 ccgctccgtt gacctcgtat tctttgccgt ctacatgaat tgtagccatt agcatgcttc
    11521 cagttttctc agtcagagac tgagcgttaa tcgaaattcg gttaccagcg ctctttcagc
    11581 aggttcggct gaatcccatt aatcaaatgg gtattgctga acggctgttt gatccccgcc
    11641 tcaaattctt cgcggaaata tttgatggcg ctctgtaacg gctccacagc accaggtgcg
    11701 tgggcacaga aagttttacc cgggcctaag aatcgacaca gttgctcaag tgtttcgata
    11761 tcgcccggct gaccttcgcc acgctccagc gcacgcagaa ttttcacgct ccacggcaga
    11821 ccgtcgcggc acggcgtaca ccagccgcag gactcacggg cgaaaaactc ttccaggtta
    11881 cgcaccagcg acaccatgtt gatttcatgg tcaaccgcca tcgccagcgc cgtacccaga
    11941 cggctgcccg ctttaccgat actttcgaat tccatcggca ggtcaaggtg cgcttcggtc
    12001 aggaagtcgg tgcccgcgcc gcctggctgc caggctttga atttcagacc gtcacgcata
    12061 ccaccggcgt aatcttcgag gatctcgcgt gcggtggtgc cgaacggcag ttcccacagt
    12121 cccggatttt tcacccgacc ggagaagccc atcagcttgg tgccagcatc tttacttttc
    12181 gagatgttct gataccactc cacgccgtta gcgaggatcg ccggaacgtt acacagggtt
    12241 tcgacgttgt tgacacaggt cggtttgccc catacgccgg aggttgccgg gaaggggggt
    12301 ttcgagcgtg ggttagcacg acgcccttcc agggagttga ttaacgctgt ttcttccccg
    12361 cagatgtagc gccctgcccc ggtatggacg aacagttcga agtcgaaacc tgttcccata
    12421 atgtttttgc caagcaatcc cgcttcagtc gcttcggcaa tggcgcggcg cagatttact
    12481 gccgcttcaa tatattcgcc acgcaggaag atgtagccac ggtaagcttt cagcgcaaac
    12541 gcggagatga gcataccttc caccagcagg tgcggcagtt gctccatcaa caggcggtct
    12601 ttataggtgc ccggctccat ttcatcggcg ttacacagca ggtaacggat gttcatggat
    12661 tcgtctttcg gcatcaggct ccacttcaag ccagtcgaaa agcccgcgcc gccgcgccct
    12721 ttcagaccag cgtcttttac ctgattaacg atttcgtccg gagacagccc ggtcagcgcc
    12781 ttacgcgccc cttcgtaacc gtttttgctg cggtattcgt ccagccacac tggctgtttg
    12841 tcatcgcgca gacgccaggt cagcggatgc gtttcgggag tacggataat gtttttcatt
    12901 tataccgctc cagcagttca gggatcgctt ccggggtcag attcgcgtga gtgtcctcat
    12961 cgatcatcat gtttggccct ttatcacagt tccccaggca gcaagttggc agcagcgtaa
    13021 agcggccatc aaatgtcgtt tgccctggtt tgatgttcag ctttttctcg agcgccgcct
    13081 gaataccctg atacccattg atatgacaga ccacgctgtc gcaataacgg atcacatggc
    13141 ggccaaccgg ctggcggaag atctggctgt agaacgttgc cacgccttcg acgtcgcttg
    13201 ccggaatacc cagcacatcg gcgatcgcgt ggatcgcacc atccggcacc cagccacgct
    13261 gcttctgaac gattttcagc gcttcaatgg acgccgcacg cgggtcttcg tagtggtgca
    13321 tctcgtgctc gatcgcttca cgctctgccg cactcagctc aaaagcctcg gtttgtggtt
    13381 gttgattctc gtgcataatt agcggtccac atctgacata acaaaatcga tactgcccag
    13441 ataaacaatc aggtcagaca ccaggctgcc gcggatcgcc gccggaattt gctgcaaatg
    13501 cgcataactc ggcgtgcgga tacgagtgcg ataactcatg gtgctaccgt cgctggtcag
    13561 gtagtaactg ttgatccctt tggtcgcctc aatcatctgg aaggattcat tggcaggcat
    13621 caccggcccc cacgacactt gcaggaagtg ggtgatcagg gtttcgatat gttgcagcgt
    13681 gcgctctttc ggcggcggcg tggtcagcgg gtgatccgct ttgaacgggc cttccggcat
    13741 gttgttgagg cactgctcaa gaatgcgcag actctggcgc agctcttcca ctttaagcat
    13801 tacgcgggtg tagcagtcag aaacaccacc accaaccggg atttcaaagt cgaagttttc
    13861 atagccagaa taaggacgcg ccttacgcac gtcgaagtcg atcccggtag cacgcaggcc
    13921 cgcgccagtg gtgccccact ccagcgcctc tttcgcgcca taggcggcaa cgccctggga
    13981 acgacctttc agaatggtat tttgcagcgc cgctttctcg taagacgcca gacgtttcgg
    14041 catccagtcg aggaactcac gcagcaggcg atcccagccg cgcggcaggt cgtgcgctac
    14101 gccgccaata cggaaccacg ccgggtgcat acggaaaccc gtgatcgctt ccaccagatc
    14161 gtaaattttc tgacgatcgg taaaggcgaa gaacacgggc gtcattgcgc cgacgtcctg
    14221 aataaaggtc gagatataca gcaggtggct gttgatgcgg aacagttcgg agagcattac
    14281 gcgaatgacg ttaacgcgag ccggcacggt gatcccggcc agtttctcta ccgccagcac
    14341 gtaaggcatt tcgttaacgc agccgccgag gtattcgata cggtcggtat acggaatgta
    14401 gctgtgccag gactggcgtt cgcccatttt ctccgcacca cggtggtggt agccgatgtc
    14461 tggtacgcag tcgacaatct cttcgccgtc gagctgcaaa acgatacgga aagcaccgtg
    14521 cgccgacggg tggttcggac cgaggttgag gaacatgaag tcctcgtttt cggtgccgcg
    14581 cttcatcccc cactcttccg gtttgaaggt cagggcttcc atctccagat cctgtttggc
    14641 tttggtcagc tcaaacggcg agaattcggt agcgcgcgcc gggtaatctt tacgcagcgg
    14701 gtgacctttc caggtttgcg gcatcatgat gcggcgcagg ttcgggtgac cgtcgaatgt
    14761 aatgccaaac agatcccagg tttcacgctc ataccagtta gcgttcggga acagtttggt
    14821 gaaggtcggt acgtgcaggt cgttttctgc cagcgccacc ttcagcatga tgtcgcggtt
    14881 acgatcgata gaaatcagat ggtagaaaac ggaaaaatcc gcggcaggta acccttcgcg
    14941 gtgcgtgcgc agacgttcgt ccatgccgtg taagtcaaac agcatgacgt aaggtttcgg
    15001 cagtttcttt aagaaatcgc caacttccag taattgttca cgcttgatcc acacaacggg
    15061 aaccccggtg cgagtcgcct gaacagtaaa ggcatccggc ccaaaacggt tgcgcagttc
    15121 gccaattacc ggatcatcaa gatgatcgcg ggtctgccag gcgggttctt gcgcggttaa
    15181 gtcggtcata ttgttcacca ttgcaaatgg tccgtggtga ctgtcggcgc agcaagcttc
    15241 gcgaaataga tttaggaata agcgaagaaa aatccctttg ccgacaggcg caaattaaat
    15301 ctcgtcaggt gtacgcaggt tggttacggc aatgcgttca ccgcgcttgc gttcgcgctc
    15361 tgattgcata ttggcgcgat aaacgccctg atcgccaacc acccaggaga gcggacgacg
    15421 ttctttgccg atagattcct gcaacagcat cagtgcctgc atgtacgctt caggacgcgg
    15481 cgggcagccc gggatataca catcaaccgg gatgaattta tcgacgccct gcacaacgga
    15541 ataaatatcg tacataccac cagagttggc acaggcaccc attgagataa cccattttgg
    15601 ttccagcatc tggtcataca gacgctgaat aaccggtgcc attttggtaa agcaggttcc
    15661 tgcaaccacc atcaggtcag cctgacgcgg cgaagcacgc aatacttctg cgccaaaacg
    15721 cgccacgtca tgcaccgcgg taaacgaagt caccatctca acgtaacagc aggaaagacc
    15781 gaagttatac ggccaaattg agtttttacg accccagtta accatgtcat tgagcttgcc
    15841 cataaacacg tttttgttaa cttcttgctc cagagggtcg gttacgatct cctgcttttg
    15901 cagggggtaa cggtcgttct caccgttggg atctatgcgg gtgagcgtat aatccatctt
    15961 aatgcctcgc ggttagcgtt gacgattagc gatactgttc gtttccgggt tcatacgctc
    16021 gcggcgtgaa cgcgcgggcg tccagtccag cgcgccaata cgcaccagat aaaccagacc
    16081 tgccagtaac acaaaaataa aaattgcagc ttccacaaag cctacccagc cgctttcgcg
    16141 gatagaggtt gaccatgcga acagatacag cgcttcaacg tcgaagataa cgaagaacat
    16201 ggccaccaga taaaacttgg cggacaggcg taagcgggcg gagccgaccg agtcgatacc
    16261 ggattcaaac ggcacgtttt tcgacctcgc gcgtgcgcga ccgcctaaaa accaaccgcc
    16321 taccagcatc aggcaacata ggccaatggc aacgataaga aagatagcga atgcccagtg
    16381 atgagcgatg acttcagtgg atgttgacat actcattgct tactcatcaa aagtagcgcc
    16441 agattcactg ctcttcacgg cagatggacg ccacatcgat tcatggggag gaataaaaaa
    16501 aaccttacaa tcactgtaga aattctttta tacagctaat tgatgtggtc ttttactcct
    16561 ttctataacc ttttgtcaac tttaacaaaa gtttcttcac attagtttac ataatatcaa
    16621 caccattagc atttaatgcc ctttcacccc agatccttga cgactccagg ataattagat
    16681 gttgttgaat cgtgtccgtt gtgaagcaat ggaaaaaata cgggtctatt ttgacaggaa
    16741 tttgtgtcga ttcctccccc caaaagagag tattttcttg atctgtgaca cgcttttgtc
    16801 attccataac aaaaacgcag caacaaattt acgtattttt taacatcatt gtagcaggtg
    16861 atttttttca ggcgattatt tgtgcgtacg gggcgtgaat ctctggtgat tgaaaaatga
    16921 acagttttgt acgttctgca cgatgtgaag aaggttcttt actaaaaaaa agccgctggg
    16981 gtttaaaaca cccccagcgg ctcgtttttt acactattgt ctcaggaatt atctatcgtc
    17041 cgtcgattac tcgatatccc tttcaatcaa caatgaatca tccccttccg gagcagatat
    17101 tgggctgtat tgccacggat tatggtagct ctccatcgcc tgatagatca cctgggccag
    17161 ctcattatta ctggacgggt catagcacag caaatattcg gtatcaggta gcggcggtaa
    17221 gccatctact ccactcagca cacgcaagtc cggactcatc atctcgaccg gccttgccgt
    17281 cacgccaaga ccggctttca ctgccgcacg aacggccgga agcgtcgagg cgacataagc
    17341 cagtcgccag ggaatatctg ctttattaag cgtcgccagc accatatcgc gaaacggact
    17401 tggatcatcc agcaatacaa gagggatcgg ctcacctttt tgcaatacgt attccgctgc
    17461 gcagtaccag tgtgttggcg aggtacgcag gttcaatgcc ttaaacgctg aaggacgatg
    17521 ggtggttacc atcaaatcca cttcctggga ttccagcatt tctgccatat aggcattacg
    17581 tttaacgcgg acatccagcg ctaatttcgg ataaaccgaa ctcacgcgat ttaacaggaa
    17641 aggtaagatc gtatcggcag attcatctga agcaccgata gttaacacgc cctgaagatt
    17701 actgaacatt aatgatgagc aggcctcatc attaaaacgc aggattttcc tggcgtaacc
    17761 aagaagttga atgccatgtt cagttaacag tttgttgcga ccgtgacgag cgaacagttc
    17821 tttcccaacg agttgttcca gacgctgcat ttgctgactt acggcggact gagtacgaca
    17881 cacagcggca gctgcggcag caaaagtgtt cagatcggca acagcaacaa atgttctcag
    17941 cagatcgagg tcgaggttaa ttatcggacg atttgcactt atcatatatt atcacttact
    18001 ggcggctcat actgagctgg ttaatgctgt gcacacacaa acaagcaatt ccatttgtaa
    18061 tgtgcctccc tggcagtttc atccggaacc cggacgaaag taaaaatgca tatgagttgc
    18121 actaaaaaag cgactcacat tgttccgtta taatgcctga agtagatcac agaatatatc
    18181 ttcagggatc gcatatctat taagttactc actcttttct atttatgaca tgcgcgtgtt
    18241 tgtataaatg taaatgtgag tccttgttcc actctcgtgc agcatcgctg gtcatacgcg
    18301 aacacgtaca aacagcaatg gtgaggcggc atcaagagca gggatccgtc atttatgcga
    18361 gcattttacc ccaaaaacct tttatttata agggtcattg cgaattatct gatgcaaagt
//
LOCUS       DAZKIP010000085        18353 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZKIP010000085 DAZKIP010000000
VERSION     DAZKIP010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.085"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(323..2011)
                     /gene="betA"
                     /locus_tag="ACHOUK_003221"
     CDS             complement(323..2011)
                     /gene="betA"
                     /locus_tag="ACHOUK_003221"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFP9898613.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAM
                     DLDNWAKEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDCKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHDLPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKMADMIRGKEALPRSTAGYFVANGMPVRAKKMSRDLN"
     gene            complement(2025..3497)
                     /gene="betB"
                     /locus_tag="ACHOUK_003222"
     CDS             complement(2025..3497)
                     /gene="betB"
                     /locus_tag="ACHOUK_003222"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFP9898614.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPSLEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCNAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGLDNGAWVAPTVFTDCSDEMTIVREEIFGPV
                     MSILTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            complement(3511..4098)
                     /gene="betI"
                     /locus_tag="ACHOUK_003223"
     CDS             complement(3511..4098)
                     /gene="betI"
                     /locus_tag="ACHOUK_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFP9898615.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRHQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            4227..6260
                     /gene="betT"
                     /locus_tag="ACHOUK_003224"
     CDS             4227..6260
                     /gene="betT"
                     /locus_tag="ACHOUK_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HFP9898616.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            6834..10817
                     /gene="ehaA"
                     /locus_tag="ACHOUK_003225"
     CDS             6834..10817
                     /gene="ehaA"
                     /locus_tag="ACHOUK_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HFP9898617.1"
                     /translation="MNKIYRLKWNRSRNCWSVCSELGSRVKGKKSRAVLISAISLYSS
                     LVFADDVIVNQDKTIDFGKENQSIDYRITVTDNANLVINATDTSRPRLTLASGGGLDI
                     TGGKVTINGPLNFLLKGTGFLNVSNAGSELYADDLYESNSGMRHDRGYFNVSNGGKIH
                     VKGTSRLTYLQGNVSGEGSQVNSETFFMGVYGSYGGNQYLSVNNGGEVNARKQISLGY
                     YDQVSDTTLAVSEGGKISAPTISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKT
                     SEKKITLNHTDKDATISADIVSGSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGAL
                     GITQNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVELTGNNYAFSGYID
                     VASGAVAVISEDKNIGRAELDVDGKLQINANKDWVFDNDLEGRGIVEINMGNHEFSFD
                     EFAYTDWFQGSLAFQNTTFNLEKNAEFLQKGGITAGQGSLVTVGKGAHSISTLGFSGG
                     TVDFGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVRSVSRDIDSALSLTEVDD
                     GNSTIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTS
                     GVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQ
                     TVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNG
                     SADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSAN
                     VLVSHAQGLGSANVENNGTLALNNSAEKRAAASVNYALGGNLTNNGTLMTGMSGQQAG
                     NVLVVKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLN
                     GIELIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDP
                     MPNPEPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGN
                     TYYTDMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSD
                     RWHVGVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQ
                     YSWFDNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTW
                     MGVKADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLH
                     NSKDFSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAM
                     VGIKWQF"
     gene            10959..12047
                     /locus_tag="ACHOUK_003226"
     CDS             10959..12047
                     /locus_tag="ACHOUK_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFP9898618.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMAGVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(12089..13020)
                     /locus_tag="ACHOUK_003227"
                     /pseudo
     CDS             complement(12089..13020)
                     /locus_tag="ACHOUK_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            complement(13112..13619)
                     /locus_tag="ACHOUK_003228"
                     /pseudo
     CDS             complement(13112..13619)
                     /locus_tag="ACHOUK_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
     gene            13877..14482
                     /locus_tag="ACHOUK_003229"
     CDS             13877..14482
                     /locus_tag="ACHOUK_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFP9898619.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNGDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            14522..15385
                     /locus_tag="ACHOUK_003230"
     CDS             14522..15385
                     /locus_tag="ACHOUK_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFP9898620.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFRLIAWQQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVIDKQNI
                     ASAVASMMGLGIGLTPSADDYLTGLALILFISGHPAGKYKEEFYLGLQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            15375..16922
                     /gene="fdrA"
                     /locus_tag="ACHOUK_003231"
     CDS             15375..16922
                     /gene="fdrA"
                     /locus_tag="ACHOUK_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFP9898621.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRGETPVDEQGLQFARGTKEAALKAVMLSGVKQEHLDLHTLNQPLITDVRA
                     RLKPQQKYIRGLFCGGTLCDETMFAVMEKHSDVYSNIQPDPEFRLKDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            16922..18340
                     /locus_tag="ACHOUK_003232"
     CDS             16922..18340
                     /locus_tag="ACHOUK_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFP9898622.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQFDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPILRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVAQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAVDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
BASE COUNT         4453 a         4515 c         4991 g         4394 t
ORIGIN      
        1 tgtgtttaaa gctccacgtt gttttggtct gacttatttt tgtcctcgtt acaagaaccc
       61 ttttcgtata tgctaaccgt gagcgggagt gaacaagtca ccatagccag gaacactagc
      121 ggcgtaattc ttaagctgag tggcatttca cctgcggttt atcagtgcat tcatagtacc
      181 ttcatcagcg agattgaacg agtcagctta gaatttgctg gcgaggttgt cactatgaag
      241 catgagagtt acccctatgt tttatataac gattctgtcc ccatacctgg aacggtaact
      301 ctcaatctga tcggttcctg agtcagttca aatcacgact catttttttc gctcttactg
      361 gcatcccatt tgccacaaaa tatcccgccg tgctcctcgg caacgcttcc ttcccgcgaa
      421 tcatatccgc cattttctcg ccaatcataa tcgtcgtggc gttcaaattc ccggtgataa
      481 tctgcggcat aattgacgca tccaccacgc gcaggccttc cagcccatgc acgcggcctt
      541 cgccgtcaac cacggacatc tcgtcgtagc ccattttgca ggtaccgcac ggatggaagg
      601 cggtttcggc gtggttacgc acaaactcat cgagctgctc atccgtctga cattccgtgc
      661 cggggctgat ttcgcggcca cgatactgat ccagcgcggg ttgatgcatg atctcgcggg
      721 tgatgcgaat tgcgtcgcgg aattcctgcc agtcctgttc gtgcgacatg tagttaaaca
      781 gaattgccgg atgctggtgc gggtcgcggg atttaatccg cacatgccca cggcttggcg
      841 agcgcatcga gccgacgtgg cactggaagc cgtgctcttt cactgcattc gagccgttat
      901 agttaatcgc taccggcagg aagtgatact gaatattcgg ccacgcaaat tcctctcggc
      961 tgcgaataaa tccgcctgct tcaaagtggt tgctggcacc aacgccggtg ccgccaaaca
     1021 gccactccgc accgattttc ggctgattcc accactgcag ggcagggtag agggaaaccg
     1081 gttctttgca ctcatattgc agatacatct ccagatgatc ctgaagattt tcaccgacgc
     1141 cgggtaaatc atgcaccagc ggaatatcaa actcggccaa cagttcagcg ttgccgacgc
     1201 cagagcgttg caggatctgc ggtgaggcaa tcgcgcctgc acataacagc acttctttgt
     1261 tcgccgtcgc gcgggtcgga atggtgctgt cgccttccag ccactcgacg cccaccgcgc
     1321 gtttacagtc aaaaataatg tgatcggtca tggcgtgagt acgaatggtt aggtttgggc
     1381 gcgatttggc ctgatcgaga taaccgcgcg cggtgctggc gcggcggccc tgcggcgtga
     1441 cggtgcgatc catcgggccg aaaccttcct gctgataacc gttgagatcg tccgtgcgcg
     1501 ggtagcccgc ctgcacgccc gcttcaatca tcgcttcaaa cagcggattg acgccgggtt
     1561 tggaggtggt gacgctcacc gggccgtcgc cgccgtggta gtcgttctcg cccacgtcgc
     1621 gggtctcggc cttgcggtag tagggcaggc aatcgagata actccagttc tccagaccgg
     1681 gttcttttgc ccagttgtcg agatccatcg cgttgccacg gatatagcac atgccgttga
     1741 tcagcgacga tccacccagg cctttaccgc gtccgcactc catgcgacgg ttattcataa
     1801 acggttcagg ttccgtctcg taagcccagt tgtagcgttt accctgtagc gggaatgcca
     1861 gggcagcggg catctgggtg cggaagtcaa agcgatagtc cgggccgccc gcttcaagca
     1921 gcagcacgga ggtattcgga tcttcagtca gacgggtagc gagaacgttg ccggctgagc
     1981 cggcaccaat aatgatgtag tcaaattgca aataaacctc ctggttagaa tatggactgg
     2041 aatttagcca tctcaacctg gatggacttc acctgggtgt aactctggag cgtcatcacg
     2101 ccgttctcgc ggccaatacc ggagtgtttg tagccgccaa cgggcatctc tgccggggat
     2161 tcgccccagg tgttgatcca gcaaataccc gcttccagct gatgaatgac gcgatgcgcg
     2221 cggttcaggt ccgccgtcac gatacccgcc gccaggccgt agtcggtatc attggcgcgg
     2281 cgaatgactt cgtcttccga ctcgtaggtc agaatggaca tcaccggccc gaagatctct
     2341 tcacgcacga tggtcatctc gtcgctgcaa tcggtgaaca cggtcggtgc aacccatgcg
     2401 ccgttatcga ggccatcgcc tttcagtaca tcgccgccgc acagtacgcg cgcgccttcc
     2461 tctttgcctt tggcgatata gcgcagcacg ttatcgcgat gcgggaagct gaccagcggg
     2521 ccgaagttag tttgcggatc gaaaacgtcg cccgcgcgaa tgcgctcaac gcgcgccaga
     2581 attttctgct caaatgcggc gttgcatttc gccggaacga agacgcgggt gccattggta
     2641 cacacctgac cggagctgaa gaagtttgcc atcatggcga tatcggcggc gagatcgaga
     2701 tccgcatcgt cgaaaacgat cagcggtgat ttaccgccca gttccatggt cacttctttc
     2761 agggaagagg ctgccgagtt agccatcact tttttgccgc tggcgacgcc gccggtaaac
     2821 gacactttgg caatgcccgg atgctcggtc agatattgcc cggtctccgc gcccacgccc
     2881 ggcaacacgt taaatacacc gtccggcagg cccgcttcgc tgtaaatttc agccagcttt
     2941 aacgcggtaa gcggagtgac ttcgctcggt ttgaaaatca tcgcattacc cgccgccagc
     3001 gccggggcgg atttccacag ggcaatctgg atcgggtagt tccatgcgcc aatccctgcc
     3061 accacgccca gcggttcgcg gcgagtgtag acaaatgatg tctcacgcaa cgggatctgg
     3121 ctgccttcca gcgacgggat cagcccggcg tagtactcca gcacgtccgc accggtaacg
     3181 atatcgacgg ttgaggtttc cgaatacgct tttccggtgt cgagggtttc cagtttggcg
     3241 agttcatcat tgcgctcgcg cagaatatcg acggcccgac gcagaatacg cgagcgttcc
     3301 atagcggtca tcgccgccca gattttttgc ccctgttggg cactcttcac ggcgcgatcg
     3361 acatcctcgc gcccggcggc ctgcacggtc gccagcacgt taccgttggc cgggttaatg
     3421 gtctcgaagg tgcgaccgct ggtggcggag gtataaccac catgtatata aagctgctgt
     3481 tctgccattc gggacatcac gtctcctcgg ttaatcggtg ggtaaatgct gagtaataaa
     3541 gtgacgggtc agcgagtgtg cgagaggttt atccagcggt ttgccgctca gtgccgcgcg
     3601 cagccataat ccgtcaatca gcgcggcaag gccgtagccc gcttcctgtg cctggtggcg
     3661 cggcaattcg cggcgaaact cgctcaccag attcgacagc aagcggcggc tgctgacctg
     3721 ctgtaaacga tagagcatcg gctgatgcat actgctggcc cagaacgcca gccaggcttt
     3781 catcgccgca ctgctcacct gcgtttcatc gaagtttccg ccaacaatcg cctgtaatcg
     3841 ctgctctgca ctgccctgcg gaagtgcatg taatcgattc aaaaccgcgt cacgcagctg
     3901 actggtgata tcgcgcatgg ttgcttccag cagaccattt ttgtccctga aatagtggct
     3961 gatgatcccc gtagaaacgc ctgcacggcg ggcgatctgc gcgatcgttg catcgtgcat
     4021 gcccacttca tttattgctt ccagtgtggc gtcgatcagt tgtctgcgcc ggatcgactg
     4081 catccccaat ttgggcattt ttgccactcc attcatcagc gttgtttatc tattaaagcg
     4141 gttattgatt ggacgttcaa tataaaatgt gtcttaattg ttacgaattt gattttaaat
     4201 ggtaacaata acagtgggga tactggatga cagacctttc acacagcagg gaaaaggaca
     4261 aaatcaatcc ggtggtgttt tacacctccg ccggactgat tttgttgttt tccctgacaa
     4321 cgatcctgtt tcgcgacttc tcggccctgt ggattggccg cacgctggac tgggtttcta
     4381 aaaccttcgg ttggtactat ctgctggcgg caacgctcta tattgtcttt gtggtctgta
     4441 tcgcttgttc gcgttttggt tcggtgaagc tcgggccaga acaatccaaa ccggaattca
     4501 gcctgctgag ttgggcggcg atgctgtttg ctgccgggat cggtatcgat ctgatgttct
     4561 tttccgtagc cgaaccggta acgcagtata tgcagccgcc ggaaggcgcg ggacagacga
     4621 ttgaggccgc gcgtcaggcg atggtctgga cgctgtttca ctacggctta accggctggt
     4681 cgatgtatgc gctgatgggc atggcgctcg gatactttag ctatcgttat aatttgccgc
     4741 tcaccatccg ctcggcgctg tacccgatct tcggtaaacg gattaacggg ccgataggtc
     4801 actcagtgga tattgcagcg gtgatcggca ctatcttcgg tattgccact acgctcggta
     4861 tcggtgtggt gcagcttaac tatggcttga gcgtactgtt tgatattccc gattcgatgg
     4921 cggcaaaagc ggcactgatc gccttgtcgg tgataatcgc cactatctcg gtgacatccg
     4981 gtgtcgataa gggcattcgc gtgttatcgg agcttaatgt cgcgctggcg ctgggattga
     5041 tcctgttcgt gttgtttatg ggcgacactt cgttcctgct taatgcactg gtgctgaatg
     5101 ttggcgacta tgtgaatcgc tttatgggca tgacgctcaa cagttttgcc ttcgaccgcc
     5161 cggttgagtg gatgaataac tggacgctct tcttctgggc atggtgggtg gcatggtcgc
     5221 cgtttgtcgg cttgttcctg gcgcgtatct cgcgtgggcg taccattcgc cagttcgtgc
     5281 tgggcacgtt gattattccg tttaccttca cgctgttatg gctctcggtg ttcggcaata
     5341 gcgcgctgta tgaaatcatc cacggcggcg cggcatttgc cgaggaagcg atggtccatc
     5401 cggagcgcgg cttctacagc ctgctggcgc agtatccggc gtttaccttt agcgcctccg
     5461 tcgccaccat tactggcctg ctgttttatg tgacctcggc ggactccggg gcgctggtgc
     5521 tggggaattt cacctcgcag cttaaagata tcaacagcga cgcccccggc tggctgcgcg
     5581 tcttctggtc ggtggcgatt ggcctgctga cgctcggcat gctgatgacc aacgggatat
     5641 ccgcgctgca aaacaccacg gtgattatgg ggctgccgtt cagctttgtg atcttcttcg
     5701 tgatggcggg gttgtataaa tctctgaagg tagaagatta ccgccgtgaa agtgccaacc
     5761 gcgataccgc accgcgaccg ctggggcttc aggatcgcct gagctggaaa aaacgtctct
     5821 cgcgcctgat gaattatccg ggcacgcgtt acactaaaca gatgatggag acggtctgtt
     5881 acccggcaat ggaagaagtg gcgcaggagt tgcggttgcg cggcgcgtac gtggagctaa
     5941 aaagcctgcc gccggaagag ggacaacagt tggggcatct ggatttgttg gtgcatatgg
     6001 gcgaagagca aaactttgtc tatcagattt ggccgcagca atactcggtg ccgggcttta
     6061 cctaccgcgc acgtagcggg aaatcgacct actaccggct ggaaaccttc ctgttagaag
     6121 gcagccaggg caacgacctg atggactaca gcaaagagca ggtgatcacc gatattcttg
     6181 accagtacga gcggcacctt aactttattc atctccatcg tgaagcgccg ggccatagcg
     6241 tgatgttccc ggacgcgtga ttgttattgc ataaaaccgc gccatgtctg catatggcgc
     6301 gggaatcatt gactcgctgt aaaaaattat gcatatatta tatatgattg tcatgtcata
     6361 tcctgaaatt cctcttgtat cacttcatga ttttatattt tattaattaa cgattttatt
     6421 ttgtattagc tgaatggatt cagtcctata gggttatata tttattaata agacgattct
     6481 tatatttgga tacctaataa gagttaccac ttttaccggc tgtatattaa atgtatttct
     6541 cctgcttttg tttatatttt gagttgattt gagttcccgg atacatataa tgcgcaggta
     6601 ttagagactg ggggggaatg taatgataaa gttaagagtg ttaacttaaa ataagttttt
     6661 agtagtaact tgctatttta ttaccctctg aactacatac tcagatatgt ggttgccaat
     6721 gctatgcaaa atttttgaat ttttatatgc gatgaacagt aaccggaatg gcatttaatg
     6781 cgttgctgtt tatgcaatct tggttttatt tggatgtatt gctggagatt gtgatgaata
     6841 aaatatatcg gctaaagtgg aacaggtccc gtaactgttg gagcgtctgc tcggagctgg
     6901 ggagcagagt aaaaggaaaa aagtcccggg ctgttttaat tagcgcgata agtttatatt
     6961 catctctggt attcgccgat gatgtcatcg taaaccagga taaaactatt gattttggca
     7021 aagagaacca gagcatcgat taccgtatta cggtgacaga caatgccaat ctggtaatca
     7081 atgcgacaga tacttcccgt ccgcgtctga ctctcgcttc tggtggtggg ttggatatta
     7141 ccggaggaaa ggtaacaatc aatggcccac ttaacttttt gctgaaaggt acggggttcc
     7201 tgaatgtctc caatgctggc agcgagttat atgctgatga tttgtatgaa tcaaactcag
     7261 gcatgagaca cgatcgcggc tattttaatg tctccaacgg cggcaaaatc catgttaagg
     7321 gcaccagccg tctgacctat ttgcagggaa atgtcagtgg tgaaggtagc caggtaaatt
     7381 ccgaaacctt ctttatgggc gtttacggca gttacggtgg taatcagtac ctgtcagtta
     7441 ataacggcgg tgaagttaat gccaggaagc aaattagcct gggctattat gatcaagtct
     7501 ccgatacaac acttgctgtt tcggaaggtg gtaaaatttc tgcgcctact attagtttaa
     7561 gcaccaactc tgagttagcg ttaggggcac aggaaggaag cgcagcgaag gcagcaggga
     7621 ttattgatgc cgaaaaaatt gagtttgtgt gggcaaagac atccgagaag aaaatcacct
     7681 taaaccacac ggataaagac gcgactattt ccgcggatat tgtcagtggc agcgagggcc
     7741 tgggctatat caatgcgctc aatggcacga cttacttaac cggtgataac tctgccttta
     7801 gtggtaaagt caaaattgag caaaatggcg ctttagggat cacccaaaat ataggtacag
     7861 cagagatcaa caaccgcggg aaattacacc tgaaggctga cgatagcatg acctttgcca
     7921 ataagatctc tggcaacggt acaataagta tcgacagtgg gacggtggag ttgaccggca
     7981 ataactatgc attcagcgga tatattgatg ttgcttctgg tgctgtcgct gttatttctg
     8041 aagacaagaa tatcggtcgt gcagagctgg atgtcgatgg caaattgcaa attaatgcca
     8101 acaaagattg ggtatttgat aacgatcttg aaggtagagg cattgttgaa ataaacatgg
     8161 ggaatcacga attctccttc gatgagtttg cttatacaga ctggttccag ggttcactgg
     8221 cgttccagaa cacgacattt aatctggaaa agaatgctga gtttctgcag aaaggcggga
     8281 tcactgcggg tcagggaagc ctggtaacag tgggtaaggg cgctcactcc attagcactt
     8341 tgggattctc cggcggaacc gttgattttg gtgccctgac agcaggtgca cagatgacag
     8401 aagggacggt caacgttagt aaaacgctgg atttgcgcgg cgagggtgtg attcaggttt
     8461 ctgacagtga cgttgtccgc tcagtatctc gtgatattga ctctgcgtta tcgctcactg
     8521 aagtcgatga tggtaacagc accattaagt tggttgatgc gcaaggtgcg gaagttctgg
     8581 gcgatgcggg caatctgcaa ttgcaggata aaaatgggca aatcctctcc agcagcgccc
     8641 aacgtgatat tcagcagaat gggcaaaaag cggccgtcgg cacttacgac tatcgtctga
     8701 cgagtggggt aaacaatgac ggtctgtata ttggttacgg cctgacccag cttgatttac
     8761 acgctaccga cagcgatgct ctggtgctga gctctaacgg taaaagcgag aatgccgccg
     8821 atctcagcgc aaagattacc ggcagtggtg acctggcatt cagcagccag aagggtcaga
     8881 ccgtatcgct ttctaacaaa gacaacgact ataccggtgt taccgatctg cgcagtggga
     8941 cgcttttgtt gaataacgat aacgtgttgg gtaataccca tgaactgcgt ctggcggcag
     9001 agactgaact ggacatgaat ggtcacagcc agaccgtggg cacgctcaat ggcagcgccg
     9061 attcactgct gagcttaaat ggcggcagtc tgacggttac caacgggggc acttcaaccg
     9121 gttcgttaac ggggagcgga gagctgaata ttcagggcgg cacgctggac atcgcgggcg
     9181 ataacagcaa cctgacggcg aatgtgaaca ttgctaattc ggctaatgtc ctggtaagtc
     9241 atgcgcaggg attgggtagc gcaaacgttg agaacaacgg taccctggcg ttgaataata
     9301 gcgctgaaaa aagagcggct gcgtctgtga attacgccct gggcggcaat ctgaccaaca
     9361 acggtacgct gatgaccgga atgtcaggac agcaagctgg caatgtgtta gtggtgaagg
     9421 ggaactacca cggtaataac ggtcaactag taatgaatac ggtactgaat ggcgatgact
     9481 cagtaaccga taaattggtt gtcgagggcg atactagcgg cacgactgcc gttacggtga
     9541 ataacgctgg cggtacaggt gcgaaaaccc ttaacggtat cgaacttatc catgtagacg
     9601 gtaagtctga gggcgaattt gttcaggctg ggcgtatcgt tgcgggggcg tatgactaca
     9661 ctctcgcgcg tggacaaggg gcaaatagtg gtaactggta tctgaccagc ggcagtgatt
     9721 ctcctgaact gcagccggag ccagacccga tgccgaatcc agagccaaac ccgaatccag
     9781 agccgaaccc taacccgaca cctacgccgg gtccggatct gaatgtggat aatgacctgc
     9841 gaccggaggc gggtagctac attgcgaacc ttgcagcagc gaataccatg ttcaccacgc
     9901 gtctgcatga gcgtctgggt aatacgtact ataccgacat ggtgacgggt gagcagaaac
     9961 aaaccactat gtggatgcgc catgaaggtg gtcataataa atggcgtgat ggcagcggcc
    10021 agctgaaaac ccaaagcaat cgctatgttc tgcaactggg aggcgatgtc gcgcagtgga
    10081 gccaaaacgg cagcgaccgc tggcatgttg gggtcatggc gggatatggc aacagcgaca
    10141 gcaaaaccat ttcctcgcga accggttatc gtgcaaaagc gagtgtgaac ggatatagca
    10201 caggcctcta tgccacctgg tatgccgatg acgagtcgcg taatggcgcg tatctcgaca
    10261 gttgggcgca gtacagctgg tttgataaca cagtgaaagg ggatgactta caaagtgaat
    10321 cctataaatc aaaaggattt accgcttcac tggaagctgg atacaaacac aaattagctg
    10381 aatttaatgg cagccaggga acgcgtaatg aatggtatgt tcagccgcaa gcacaggtta
    10441 cctggatggg agtcaaagcc gataagcacc gcgaaagcaa cggaaccctc gttcatagca
    10501 acggtgatgg caatgttcaa acccgacttg gcgtaaaaac ctggctgaag agccaccata
    10561 aaatggatga cggtaaatcc cgcgagttcc agccgtttgt agaagtgaac tggctacata
    10621 acagtaagga tttcagcacc agtatggatg gcgtgtctgt cactcaggat ggagcccgaa
    10681 atattgctga gataaaaacc ggggtggaag gacagctaaa tgccaacctg aatgtctggg
    10741 ggaatgtggg cgttcaggtt gccgataggg gatataatga cacctctgca atggttggca
    10801 ttaagtggca attctgatct gaataatgag taaaaggaga gagagccgaa cgggagataa
    10861 ttcggctctc tgtgtcattt tcttgcagcc agctttatcc gtcagagtga agactcgcag
    10921 atttagctgg cgcacaagat ttaatcaagg tgtgttcaat ggcttcgcat gatttgagtg
    10981 tttttttgga agagttcggg gcaacggtga atttgacgtt gcctggtatc gtgtccgaaa
    11041 aagaacggct tttactcaag ctgctaatgg agggaatgtc ggttacagaa atctcacagt
    11101 acagaaatcg cagtgcaaag acaatttcac atcaaaagaa gcagctgtat gaaaaactag
    11161 gcatccagag tgatattacc ttctggcggg atatcttttt tcaatatcat ccgcaagtca
    11221 ttagtggcac gggaaataaa aataattttt atatccctga taaccgttgt caccatatcg
    11281 tcacgcctga agccatcagt ctggcgttgg aaaaccatga atttaaaccg tggatccaac
    11341 cggttttctg cgcgcagact ggggtactga cgggctgtga agtgcttgtc cgctgggaac
    11401 atccacaaac gggaatcatc ccgccggatc agtttattcc tctggcggag tcatccggtc
    11461 ttattgtcat aatgacccgc caactgatga aacagactgc ggatattctg atgccggtaa
    11521 aacatttgct gccggataat ttccatattg gtatcaacgt ctcggcgggt tgttttttgg
    11581 ccgcgggatt tgaaaaagaa tgcctgaacc tggtcaagaa attaggaaac gataaaatca
    11641 aactggttct cgagctaacg gaacgtaacc ctattccggt aacgccagaa gccagagcga
    11701 tatttgacag ccttcatcag cataacatta cctttgcgct ggatgacttt ggtacgggtt
    11761 atgcgaccta tcgttatctg caggcgtttc cggtcgattt tattaagatc gataagtcat
    11821 ttgtgcaaat ggcgggtgtc gacgaaatat ccggtcatat tgtggacaat attgtcgaac
    11881 tggcgcgtaa gcctggtctg agtatcgtgg cggaaggggt agaaacccag gagcaggcgg
    11941 atttaatgat cggtaaaggc gttcactttt tgcagggcta tttgtactct ccgccagtac
    12001 cgggtaataa atttatctct gaatgggtaa tgaaagcagg tggttgatgt aaaccgctat
    12061 tcacagcgca tcgggaggtt ggcagcgatt aattctcccg atgcagtaaa tcctgataga
    12121 tccccgttaa tatgccattt ggcgcaaatt gttttttaat ccactgcgtc acctgacccg
    12181 tagcagaatg ctgagtcgcc agtaacatgc gtgaatcctg gcgcgggtta tggatttgtc
    12241 gggtaaccag cagggattgc gccatcgcct cacggaccat gtaatctggc aaaaaaacaa
    12301 tcccttcacc taaaatctgg cactgacatt tggtgttgaa atctggcacc agaattgact
    12361 cctgcccgtg cagcaaccag cccacttttt tattaatcgt atgcgcggtg tcctccacca
    12421 taatattagg gtagagacgc agttggcttt cggcgatggt tccggcacaa aggctaacgg
    12481 gtgatccggg gcgatagcaa aagcccagcg aatcgcgcca atttcagtgt aatcaatacc
    12541 gccgccgtcc agcagcgtgt cgggtgcgcc gatagcgata tttgcctgat tattaataat
    12601 cgcatcccag acgccgttat acacttcggt ggtgacggta atctggcagg taggaaactg
    12661 ctttttcagc acctgtaata agcgtgcggt gtgtttgggc gtataaagca actgattaat
    12721 acaaatgcgc acacgcgctt ctatgccctg cgaaatagta tcgatgctgc gtttgatggc
    12781 gtgaaaatca ttcagcaggt cggtagcttt gcggaaaaaa taacgcccgg actccgttaa
    12841 ctcaatgctg cgggtactgc gagtgaacag caccacatcc agccctgttt ccattcgctt
    12901 gatggtgtag ctaatggcgg aagtggttaa acccaactct tctgcggctt tactgaaact
    12961 gccaaaacgc gcagcggtag taaatgccaa cagattctct tcggtaaaaa ttgagttcat
    13021 taaaccattc ccgccattat ctaatatgaa tttctccaag cataactgta gaggtatcaa
    13081 ttagagatgg gtaatcattt ttgaataact tttaataccc gtcgcgttta gtcgtcgctt
    13141 cgcttaattt ttgtccgcca tattctgaaa ttatccccac gcagttaccc gccagaaaac
    13201 ccagtaacgt caccgtccag ttcatcccgc tggcaaaaaa cagcgtcatg ccgagaaagc
    13261 caccgggaat aaatgacagt aaccagaaac gcccttgcca gaccaccacc gccgagaagg
    13321 gtacggtagt aattacgctc gcccagaacg cggacagtcc acttacagag gccagccaac
    13381 cggcagccag cgcggcgaaa aacgcccaca ccatcccgga ataattaacc gacagacttc
    13441 tgataaaccc cgatttaccg ccgcctgcgg cataaaaact gcaaaaggcg acgaagccaa
    13501 tggttcctaa taattcctaa taattcccat ccctgagaga gcccgacatg tgaagacact
    13561 aactgccaaa gccctgcaca aataccgacg gtgacacccg ttgccgtgag tccgttcatc
    13621 ttgttcccct ctggttagac atggacgaaa gctactctta catgtgcaga ctatagagaa
    13681 ataaaacctg attgagtgaa agggtatcgg gtcaaagaaa caaatattgc actaccgcac
    13741 actgcgaaaa gattgttgaa catcattcaa caaaaaggcg aaatacggcg aattaaattt
    13801 aagcgggttc ttttatcaga ttatcgttgc tattctcaat tcatcgaaaa cagccacaaa
    13861 aaccagggga atgtgaatga gtataaaaaa tctacctgcc gactatttat tggctgcaca
    13921 acagggtgat atcgataaag taaaaacctg ccttgcgctg ggtgtcgata ttaatacctg
    13981 cgatcgtcag gggaaaacgg caattacgct ggcaagttta tatcagcaat atgcttgcgt
    14041 tcaggcatta attgatgccg gagcggatat taataagcaa gatcatacct gtttaaatcc
    14101 ttttttaatt agctgtctga acggtgattt aacgctgcta cgaattattt taccggctaa
    14161 accagacctt aattgcgtca cccgttttgg cggtgtcggc ctgacgcctg cctgtgaaaa
    14221 aggccattta agtattgtaa aagagctttt ggcgcatacg gaaattaacg ttaaccagac
    14281 caaccatgtt ggctggacgc cactactgga agcgattgtg cttaacgatg gcgggattaa
    14341 acagcaggcg attgtgcagt tattgctgga acacggtgcc agcccgcatc tgaccgataa
    14401 atatggcaaa acgccactgg aactggcgcg ggaacggggc tttgaagaga ttgcgcagtt
    14461 actgattgcc gcaggtgcat aaaccgggag gcttgctatc aacacaccag aaaaacggtg
    14521 tgtgtgggcg ctaactgcgg atgcggattt tctggcgcag cgagggcaag gacaggttga
    14581 acaggtcttt gccagagcgg taaatatcgc actcccggct cgccagcagt tgctgacgct
    14641 gctttgtgaa gagtacgaca atgcgccaaa cagttgtcgg ttggcactca ctcactttga
    14701 tgatctgttc cggcatggtg ataaggttca gtttgacgat caaggtatta cggttggtca
    14761 acatcttcat atagagatga gtcgttgtcg gcgttggctg tccccaacct tgcaaatgac
    14821 cgctgtgaat tttcgcctta tcgcctggca acagtggcac gacatcattc atcagcacct
    14881 gggggaaaat gaaaccctgt ttaattatcg cggcgataat ccgttttatc aggcgttaaa
    14941 taaagaatta catattaaac gacgggcagt tatacaggca gtaatcgata aacaaaatat
    15001 cgcctcagcg gtcgccagta tgatgggctt agggattggc cttacgccat cagccgatga
    15061 ttatttaaca ggtctggcac ttattttatt tatttccggg catccggcgg gaaaatacaa
    15121 agaggaattt tatctcggct tgcaacgcgg cagaaataat accacgttat taagtgccat
    15181 aacgctggaa gccgcattac aacaacgctg ccgggaaaat attcatcgtt ttattcacaa
    15241 cattatttat gacatccctg ggaacgcaac tcaggcaata gaaaaaatta aacatattgg
    15301 ctccagttcc ggctgcgaca tgctgtatgg catggccgat ggttgtgcgc tgagccaaac
    15361 ctacggaggg aattatgtca gttaaaatag tcattaaacc gaatacctat tttgattctg
    15421 tctcgctgat gtctatctcc acgcgtgcaa ataaactcga cggcgtcgag caggcatttg
    15481 tggcgatggc gaccgaaatg aacaaaggtg tgctgaaaaa tttaggactg ctgacgccgg
    15541 agctggagca ggcgaaaaac ggcgacctga tgattgtcat caatggtaaa tcgggggcgg
    15601 acaacgagca gttgctggtg gagattgaag aactgttcaa caccaaagcg caaagcggct
    15661 cgcacgaggc gcgttacgcc actattgcca gcgccaaaaa gcatatcccg gaaagtaacc
    15721 tggcggtgat ttcggtcaac ggtctgtttg ccgctcgcga agcgcgtcag gcgctgcaaa
    15781 acgatctcaa cgtgatgctg ttttccgata acgtctcagt tgaagatgaa ctggcgctca
    15841 agcaactggc ccacgaaaaa gggctgctga tgatggggcc agactgcggc acggcgatta
    15901 tcaacggtgc ggcgctctgc tttggtaacg ccgtgcgtcg cggcaacatc ggtattgttg
    15961 gcgcatccgg caccggcagt caggagttga gcgtccgcat tcatgaattt ggcggcggcg
    16021 tttcgcaact gattggcacc ggcgggcgcg acctgagcga gaaaatcggc ggcctgatga
    16081 tgctcgacgc catcgggatg ctggaaaacg atccgcaaac tgaaatcatt gcgcttatct
    16141 ccaaaccgcc tgcgcctgcg gtggcccgca aagtgctgga acgtgcgcgc gcctgccgca
    16201 agccggtggt cgtttgcttc ctcggtcgtg gcgaaacgcc agtggatgag caggggctac
    16261 agtttgcccg cggcaccaaa gaggcagcgc taaaagcggt gatgctctcc ggcgtgaaac
    16321 aagaacatct cgacctgcat accctgaacc agccgttgat tactgacgtg cgcgctcgcc
    16381 tgaagccgca gcagaaatac attcgtggcc tgttctgcgg tggcacgctg tgcgatgaaa
    16441 cgatgttcgc ggtgatggaa aaacacagcg atgtctacag caacatccag cccgatccgg
    16501 aattccgcct gaaggatatc aaccgcagca tcaaacacac cttcctcgac tttggcgatg
    16561 acgacttcac caacggcaag ccgcacccga tgattgaccc taccaaccgc atcagccgct
    16621 tgatcgaaga ggcgcgcgat ccagaagtgg cggtgatcgt gatggatttt gtgctcggat
    16681 ttggatcgca tgaagatccg gtcggttcca ccatcgaggc gatcaaagaa gcgaaagcga
    16741 tcgccgctgc cgaaggacgc gagttgatca ttctcgccta tgtgctgggt accgatcttg
    16801 atacgccatc gttagaacaa caaagccaga tgctgcttga tgccggcgtg attctggcga
    16861 gcagcagcac caataccgga ttgctggcgc gtgaatttat ctgcaaaggg gaggaagcct
    16921 gatgagccag tcactgttta gccaaccatt gaacgttatt aacgtcggca tcgccatgtt
    16981 tagcgatgac ctgaaaaagc agcatgtaga agtgactcaa ttcgactgga cgccgccggg
    17041 gcagggcaat atgcaggtgg tgcaggcgct ggataacatt gccgattcgc cgctggcaga
    17101 caaaatcgcc gccgctaacc agcaggcgct ggagcgcatt atccagtcgc atccggtgct
    17161 gattggtttt gatcaggcga ttaacgtggt gccgggcatg acgccgaaaa ccattcttca
    17221 cgccgggccg ccgatcacct gggaaaaaat gtgcggcgcg atgaaaggcg cggtcaccgg
    17281 agcgctggtg ttcgaaggac tggcgaaaga tctcgacgag gcggctgaac tggcggcttc
    17341 cggggagatc accttctcgc cgtgtcacga gcacgactgc gtgggatcga tggcgggtgt
    17401 gacctcggcc tcgatgttta tgcacatcgt aaaaaacaaa acctacggca acatcgctta
    17461 taccaacatg agcgagcaga tggcgaagat tttgcgtatg ggcgctaacg accagagcgt
    17521 gattgaccgc ctgaactgga tgcgtgatgt gcagggacca atactgcgcg acgcgatgaa
    17581 aattatcggc gaaatcgatc tgcgcttaat gctggcgcag gcgctgcata tgggcgatga
    17641 gtgccataac cgcaataacg ccgggacgac gctgctgatt caggcgctga cgccggggat
    17701 tatccaggcg ggctattccg tcgcgcaaca gcgcgaagtg tttgagtttg ttgccagtag
    17761 cgattacttc tccggcccga cgtggatggc gatgtgtaaa gcggcgatgg atgcggcgca
    17821 cggcatcgaa tacagcaccg tcgttaccac catggcgcgt aacggcgtcg agttcggcct
    17881 gcgggtgagc ggcctgccgg ggcagtggtt taccggccca gcgcagcagg tcatcggccc
    17941 gatgtttgct ggttataagc cggaagattc ggggctggat atcggcgaca gcgccatcac
    18001 agaaacctac ggtattggcg ggtttgccat ggcgaccgcg cctgcaatcg tcgcgctggt
    18061 ggggggcacg gtggaagagg ctatcgattt ctcccgtcag atgcgcgaaa tcacccttgg
    18121 cgaaaacccc aacgtcacca ttccgctgct cggttttatg ggcgtgccgt cggcagtcga
    18181 catcacccgc gtgggtagca gcggcattct gccggtgatc aacaccgcca tcgcccataa
    18241 agatgcgggc gtcggcatga ttggcgcggg cattgtgcat ccaccattcg cctgcttcga
    18301 gaaagccatt cttggctggt gcgaacgtta cggcgtttga cctacctgtt cat
//
LOCUS       DAZKIP010000086        18316 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZKIP010000086 DAZKIP010000000
VERSION     DAZKIP010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.086"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(160..966)
                     /gene="trpA"
                     /locus_tag="ACHOUK_003233"
     CDS             complement(160..966)
                     /gene="trpA"
                     /locus_tag="ACHOUK_003233"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFP9898623.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADTLELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPKKMLAALKAFVQPMKAATRS"
     gene            complement(966..2159)
                     /gene="trpB"
                     /locus_tag="ACHOUK_003234"
     CDS             complement(966..2159)
                     /gene="trpB"
                     /locus_tag="ACHOUK_003234"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFP9898624.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(2171..3532)
                     /gene="trpCF"
                     /locus_tag="ACHOUK_003235"
     CDS             complement(2171..3532)
                     /gene="trpCF"
                     /locus_tag="ACHOUK_003235"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFP9898625.1"
                     /translation="MMQTVLAKIVADKAIWVEARKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVICDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLKMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFADGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVLNKAKVLSLVAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(3533..5128)
                     /gene="trpD"
                     /locus_tag="ACHOUK_003236"
     CDS             complement(3533..5128)
                     /gene="trpD"
                     /locus_tag="ACHOUK_003236"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFP9898626.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEHLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(5128..6690)
                     /gene="trpE"
                     /locus_tag="ACHOUK_003237"
     CDS             complement(5128..6690)
                     /gene="trpE"
                     /locus_tag="ACHOUK_003237"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFP9898627.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQSLSGNGEALLTLLDNALPAGVENEQLPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTETAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLESRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(6712..6805)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(6782..6826)
                     /gene="trpL"
                     /locus_tag="ACHOUK_003238"
     CDS             complement(6782..6826)
                     /gene="trpL"
                     /locus_tag="ACHOUK_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFP9898628.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            6964..7845
                     /gene="rnm"
                     /locus_tag="ACHOUK_003239"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             6964..7845
                     /gene="rnm"
                     /locus_tag="ACHOUK_003239"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFP9898629.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAHIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HDGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            7842..8462
                     /gene="yciO"
                     /locus_tag="ACHOUK_003240"
     CDS             7842..8462
                     /gene="yciO"
                     /locus_tag="ACHOUK_003240"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFP9898630.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            8490..10073
                     /locus_tag="ACHOUK_003241"
     CDS             8490..10073
                     /locus_tag="ACHOUK_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HFP9898631.1"
                     /translation="MAGKFRCILLLIVGLFFSSLSYAKNTEMPSYEEGISLFDVEATL
                     QPDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGIPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNHWPF
                     EIYKTRFSLKFPDIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESSDPFYREDFTVL
                     YRWPHSLLSNAPAPQTTNIFSHLLLPSTSSLLIWFPCLFLVCGWLYLWKRRPQFTSVD
                     VLRMQKFYLPRKKSSFYRPDTFLQWGGLAILAVILYGNLSPVGWAGMSLVGDMFIMIC
                     WLLPFLFCSLELLFARDDDKPCVNRVIITLFLPLICSGVAFYSLYINVGDVFFYWYMP
                     AGYFSAVFLTGYLTGMGYIFLPKFTQTGQQRYAHGEAIVNYLARKEAATHSGRRRKGE
                     TRKLDYALLGWAVSANLGREWVARITPSLTAAVRAPEIARSGVLFSLQMHLSLGANTS
                     LLGRSYSGGAAGGGGGGGW"
     gene            10286..11158
                     /gene="rluB"
                     /locus_tag="ACHOUK_003242"
     CDS             10286..11158
                     /gene="rluB"
                     /locus_tag="ACHOUK_003242"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFP9898632.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(11198..11788)
                     /gene="cobO"
                     /locus_tag="ACHOUK_003243"
     CDS             complement(11198..11788)
                     /gene="cobO"
                     /locus_tag="ACHOUK_003243"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFP9898633.1"
                     /translation="MSDERYQQRQQRVKEKVDARMAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDLVLLDELTYMVAYDYLPLEEVLQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(11785..12543)
                     /gene="yciK"
                     /locus_tag="ACHOUK_003244"
     CDS             complement(11785..12543)
                     /gene="yciK"
                     /locus_tag="ACHOUK_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFP9898634.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCRSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVIQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            12763..13812
                     /gene="sohB"
                     /locus_tag="ACHOUK_003245"
     CDS             12763..13812
                     /gene="sohB"
                     /locus_tag="ACHOUK_003245"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFP9898635.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVSTDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(13848..14099)
                     /gene="yciN"
                     /locus_tag="ACHOUK_003246"
     CDS             complement(13848..14099)
                     /gene="yciN"
                     /locus_tag="ACHOUK_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFP9898636.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            14479..17076
                     /gene="topA"
                     /locus_tag="ACHOUK_003247"
     CDS             14479..17076
                     /gene="topA"
                     /locus_tag="ACHOUK_003247"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFP9898637.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            17286..18260
                     /gene="cysB"
                     /locus_tag="ACHOUK_003248"
     CDS             17286..18260
                     /gene="cysB"
                     /locus_tag="ACHOUK_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFP9898638.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
BASE COUNT         4447 a         4554 c         4861 g         4454 t
ORIGIN      
        1 ttgtttgatt atattactgt tgggcggaaa aatgacgtaa gttgacgttc gacaggggta
       61 agcgaaacgg taaaaagata aatattaaat gaatttagga tttttccggc ttcgttaaag
      121 aaagttaaaa tgccgccagc ggaactggcg gctgtgggat taactgcgcg tcgccgcttt
      181 catcggttgt acaaaagctt tcagtgccgc cagcattttc tttggctcat taatatgttg
      241 ctcgatgatt ttaacaatcg cagaaccaga aatcgcgccc gcagctcctg catcaatcgc
      301 tgcttttacc tgatccgggg cggaaatacc aaatccctgc aatggaggtg ccgcgttgta
      361 ctctttcagc ttcgcaacca gatgattgag gggtaacgcg gcgcggtttt ctgcgccggt
      421 cacgcccgct cgtgacagca aatatgtgta accacgaccg taagaggcta tctggcgcag
      481 caggtcgtca tcggcatttg gcgggcagat gaagataggt gcgacattat gacgcaacgc
      541 ggcctggcgg aagggcgcgg actcttcaac tggcacatcg gcaaccagca ccgaatcgac
      601 gccgactttc tcgcactggg cataaaactc atcaatgcct ttgttaaaca ccaggttggc
      661 atacatcagc aggccaatgg gaatggtcgg gtgtttctgg cgaatgagtg ccagcatctc
      721 aaagcactgc gccggggtca cacctgccgc aaaggcacgc agtgtggcgt tttgaatcgt
      781 cgggccatct gccagtgggt cggagaaggg gatgcctaac tccagcgtgt cagcaccggc
      841 ttcaattagc gtatcgataa ttttcagcga ctgctcaatg cccggatcac cgagcgtgac
      901 gaaaggaacg aatgcgcctt ctttgcgctc cttcaactgg gcaaacagag attcgtagcg
      961 ttccatcaga tttcccctcg tgctttcaaa atatcgtgaa cggtgaagat gtctttatcg
     1021 ccgcgaccgg aaaggttaac caccagtagc tgctcttttt ccgggttttc gcgcatcatt
     1081 ttcagcgcat gggccagggc gtgggaggat tccagcgccg ggataatccc ttcgtgcagg
     1141 cacagcgttt tgaaggcttc cagtgcttca tcatcggtaa ttgacacata atcagcgcgt
     1201 ccagtgctgt taagatacgc gtgttgtggg ccgacggacg gaaaatcgag tccggcggag
     1261 atggagtaag attcttcaat ctgcccgtct tcggtttgca tcatcggcgc tttcataccg
     1321 aaatagatac ccacgcgacc atgttttagc ggtgcgccgt gctcgccagt ttcgataccg
     1381 tgaccaccag gctccacacc aatcaggccg acgttggttt cgttgatgaa atctgcaaac
     1441 atgccgatgg cattcgaacc gccgccaaca caggcgataa cggcatccgg caggcgacct
     1501 tctctttcca gaatctgtgc tttggtttct tcgccaatca tccgctgaaa ctcacggaca
     1561 atggtcggat aaggatgtgg gccagctgcg gtgcccagca tatagtgcgc ggtttcataa
     1621 ctgccggacc agtcgcgcag cgcctcgtta caggcatctt tcagcgtcgc ggaaccgcta
     1681 tgcaccggga tcacttccgc acccattaaa cgcatacgaa aaacgttagg cgactggcgt
     1741 tcaacgtctt tggcccccat ataaatacgg catttcaggc cgagcagggc gctggcaagg
     1801 gccgacgcca cgccatgctg accggccccg gtttcggcga tgatttcggt tttacccatc
     1861 cgcttcgcca gtaaagcctg accgagcacc tggttagttt tatgcgcgcc gccgtgcagc
     1921 aaatcttcgc gcttcagata cagcgtggtg ttcgtgccag ccgtaatgtt ctggcatttg
     1981 gtcagcgcgg ttggacgccc ggcatagttt ttcagcaggt cgttgaactg agcctgaaat
     2041 tcaggatctt tttgcgcgct gacaaaagct tcttccagct ggcgcagagc aggcatcagg
     2101 atttgtggca cgtacatgcc gccaaactca ccaaaatagg ggttaagtaa tgttgtcatt
     2161 gttcctttcc ttaatatgcg cgcagcgtct ggaaaaccga ggccaaaaga cgtgcgtctt
     2221 tgatgcccgg ttgcgactct acagcagaat taaaatcaag tccggcgcag ccggtttgtg
     2281 ccgcttccac gcagttatct gcgcctaagc cccccgccag cagaacgttg ccaagcgatt
     2341 gaccatttaa tagtgaccag tcgaaacgtt gcccgcttcc accctggccg ttgtctaaaa
     2401 catatttatc aacgtgctga agctcgcggg cgggcagggt ttcaccgacg cttaatgctt
     2461 tccagatggc aacgtgtgct ggcagagctt cacgcagcgt atcgatatac agctgatctt
     2521 cattaccatg cagttgcact gccaccagtg ataacacctt agctttgttc agcacatcgg
     2581 caatatcgtg attgcggaac acgccaacat actgcaacgg tgccgcagcc atcacttcct
     2641 gcgcctgttc aacgttgacg caacgcggtg atgtcgcaac aaaaatcaac ccaccgtaaa
     2701 tcgcgcccgc gtcgtacgct gctttagcat cttgcccacg cgtcaggcca catactttat
     2761 tctcacccag caacacccga cgcacggcgg cgtgcaaatc gtcatgggcc atcaacgccg
     2821 aaccaattag aaaaccgtca gcgaagtggc ttaactcgcg cacctgagcg taagtattga
     2881 tgcctgattc gctgattacc gtcacgttgt gccccagttt cggcgcaagc tcgcgggtac
     2941 ggttgagatc aatcgacaaa tcacgcagat cgcggttgtt gatgccaacg acctttgccc
     3001 ccaatgcaat ggcgcgctcc agttcctctt cattactgac ttcggtcagc acacccatct
     3061 tcagactgtg ggcgacggcg gcaagctggc gatattgttc gtcatccagt actgaaagca
     3121 ttaataagca ggcatcggcc tggtaatagc gcgccagata gatctggtaa gggtcgataa
     3181 tgaagtcttt acataaaatc ggctgcgggg cgatttggct gacgatgggg aggaaatcaa
     3241 agctcccctg aaaatatttc tcatcagtca gcaccgaaat tgccgaagcg taatgtttat
     3301 aaatggcggc aatgcgtgct ggatcgaaat catcacagat cacgcctttt gacggcgacg
     3361 ctttcttgca ctccagaata aacgccgtgc gcgcaccctg tagcgcatca taaaaatgtc
     3421 gcgtgctcgg ctgaacctca ttctgaaaac tggccagcgg ttgctgctgt ttgcgggctt
     3481 ctacccaaat cgccttgtct gcgacgattt tcgctaaaac ggtttgcatc atttaccctc
     3541 gtgccgccag tgcggtgact ctgtcgtaag cggaaccact gcgcagtacc tcaagaacgg
     3601 tttgcgcatt ggcttgcaga tcttcatggc catgcaggcg cattaacatg gcgacattcg
     3661 ccgcgacggc tgcttcatgg gcggcgtcgc ctttaccttg taacaagcgt gttaaaatgt
     3721 cacggttttc ttccggtgtt ccgcctgcca gttgctcctg gtggtaggga gtcaggccaa
     3781 aatcttcagc ggtcaattga tagctcttaa tttcgccgtc atgcagctcg gcaacgattg
     3841 tcggcgcgtg taatgaaact tcatccatcc cgccgctgtg caccaccgcc gcgcgttgat
     3901 accccagcac gcgtaaggtt tcggcaatcg gcagcaccag ttccggacta taaacaccaa
     3961 ttaacgccag cggcggatgc gccgggttaa tcaatggccc cagcacattg aacagggtgc
     4021 gggtttttag ttgctggcga accggcattg catggcgaaa tccggtgtga tatttcggtg
     4081 caaagaggaa acatacacct aactcatcca gcgcctggcg cgatttatcg gcgttcatat
     4141 caagattaat accgaacgcc gccagcagat ccgacgagcc agatttactg gagacgctac
     4201 ggttgccgtg tttcgccact ttcagcccac aggccgcggc gacaaacgca ctggcggtgg
     4261 aaatattgat gctgttgctg ccgtcaccgc cggtgccgac gatatcggca aacagataat
     4321 ccggacgcgg gaacggcgcg gcgttttcca gtagcgcggt tgctgctccg gcgatctcgt
     4381 tcgggtgctc gccgcgaatt ttcatgctca ccagcgccgc cgccaggtgt tccggtttca
     4441 gttcaccacg taccaccgct gaaaatagct gatgactttc ctgctggcta agggtctgcg
     4501 cctgatacag tttttccaga atcggttgca gcgtgttggt tggctctagt ttctgctgcg
     4561 cccaggccag cgtttgttcc agcaggcgag cgccctgggt ggtgagaatg gattccgggt
     4621 ggaactggaa tccgcaaacg cgatctgcat cgtgacgcac cgccatcacc atgccattaa
     4681 aatgggcgtt gatggttaaa ccggccggaa tattactgcc aaccagcgag tgataacgcg
     4741 ccaccggcag cgggtttgtt aatccggcaa acatcgcctg accgtcatgt tcaatgctcg
     4801 acgctttacc gtgaagaatt tcgcccgcct gaccgacata gcccccgtaa gcttcgacaa
     4861 tcgcctgatg accaaggcaa atgccaataa taggcagctt accgcgcagg cgggtaagca
     4921 gttcaggcat acaaccagct tcgctcggca caccgggtcc aggagaaagc atcagcaccg
     4981 gattgctcat cgtcgcaagg cgttcaatta aggtctgcgc cggaatatgg ttgcggtaaa
     5041 tcaccacgtt atgaccattg ctgcgcaact gatctgccag gttgtaagta aaagagtcga
     5101 tattatcgag cagcagaatg tcagccatta gaacgtctcc tgtgcatgat gcgcggtggc
     5161 aatagcgcgc agtacagcgc gggctttatt acgggtttcg tcggcttccg actgcggaac
     5221 agaatcaagg actacgccag caccggcttg cacggtagcg ataccgtttt ccaccagcgc
     5281 cgagcggatc acaatgcagg tgtcgagatc gccgtgcgcg gtgaaataac ctaccgcgcc
     5341 gccgtagctg ccgcggcgac gaccttcagc ctcggcaatt aactgcatag cgcgtacttt
     5401 cggcgcaccg cttaacgtcc ccatattcat acaggcgcgg taagcgtgca gggcgtcgag
     5461 atcgtggcgc agctcaccga ccacgcggga gaccaggtgc atcacgtaag agtaacggtc
     5521 aactttggtg aggtcggcga cgtagcggct gccgggggtg caaatgcgtg ccaggtcatt
     5581 acgggcgaga tccaccaaca tcagatgttc agaaagctct ttatgatcgg tacgcatttc
     5641 cagttcaata cggctttcaa ggtccctgtc gagcgagcca tcggcgcgac gaccgcgtgg
     5701 acgtgttccg gcaatcgggt agatctcaat ctggcggctg gtggcgtcat acttgagcga
     5761 actttccggc gacgcgccaa acagggtgaa atcattatcc tgcataaaaa acatgtacgg
     5821 gctgggatta ctctttttca gcacgtaata ggccgccagc ggtgacgggc agggcagaga
     5881 gaaacggcga gatggcacca cctggaaaat ttctccggcg cgaatcgctt tttgcaacaa
     5941 acgcactacg ccaccgaact cttcatcgct ctggttacat tcacaacgca tatgcggcac
     6001 ggaaaccacc ggcagcggtg gcgcggtttc ggtcagttgc tggcgaagat cgttcaggcg
     6061 agcagtgaga cgttgttttt cttcttcatt cggagcaaac aggctggcct gaatgcgggt
     6121 gctttttttc tgatggtcaa tcaccatcag agtttcagcg agataaaaac agaaatcagg
     6181 gcagttattt tccgctgaca gttgcggtaa atcttcaaac cccgccacaa ggtcataaga
     6241 gaacaggccg ccgaagaaca tggcttctcg ttcttccttc ggtacattca acagattctg
     6301 caataagcgg aaagcgtcaa aaaccgaaag ggagcataag cgagcgtctt catccagcag
     6361 tggactaaca ggggggaagc gcagcacacg gcagtttggt aattgttcat tttccacacc
     6421 cgcaggcagg gcgttatcca gtagtgtcag cagggcttcg ccgttgccgg aaagggactg
     6481 gattgtgaca gtgtcaccta aagctgtaat gcgcagcgca ctgtctacca gcagcaggct
     6541 ttttaaatca tctttgctgt cgatatctgc ggattccagc agcagcgttg ccggacgatc
     6601 cccacacaat tggtgaaaaa gcgcggtggg attgtcgcga taagcgcctt cgcaggttag
     6661 cagttcgaga gtcggttttt gtgtttgcat tgttattctc taattttgtt caaaaaaaag
     6721 cccgctcatt aggcgggctg ggtatctgat tgctttacgc atggtgaata cactgcccgt
     6781 ttcaggaagt gcgccaccaa cctttcagta cgaaaattgc tttcattgtc gatacccttt
     6841 ttacgtgaac ttgcgtacta gttaactagt tcgatgattg attgtcaaca gctcatttca
     6901 gaatatttgc cagaaccgtt atgatgtcgg cgcaaaaaac attatccaga acgggagtgc
     6961 gccttgagcg acacgaatta cgcagtgatt tacgacctgc acagccatac cacagcttcc
     7021 gatggctgcc tgacgccaga agcattggtg caccgtgcag tcgagatgcg cgtcggcacc
     7081 ctggcgatca ccgaccatga caccacagct gcaatcgcgc cagcaagaga ggaaatttca
     7141 cgttcgggac tggcgctgaa tcttattccc ggcgtggaaa tttccacggt ctgggaaaat
     7201 catgaaattc atattgtcgg gctgaatatt gatattactc atccgctaat gtgtgagttc
     7261 ctcgcgcagc agacagaacg gcgcaatcag cgggcgcagc aaattgccga aagacttgaa
     7321 aaagcgcata tacctggggc gctggaaggc gcacaacgac tggcgcaggg cggcgcagtg
     7381 acgcgcggtc attttgcgcg tttccttgtt gagtgcggca aagccagttc aatggcggat
     7441 gtctttaaaa agtatctggc gcgcgggaaa accggatacg ttccgccaca gtggtgtaca
     7501 atagaacaag ctattgatgt cattcatcat tctggcggta aggcggtgct agctcatcct
     7561 gggcggtaca atcttagtgc taaatggctg aaaagattgg ttgcgcattt tgccgaacac
     7621 gacggcgacg cgatggaagt cgcgcagtgc cagcaatcgc ccaatgaacg tacccagctg
     7681 gcgacgcttg cccgccagca ccatttatgg gcatcacaag gatctgattt tcatcagcca
     7741 tgcccgtgga tcgaactggg tcgtaaactc tggctgcccg caggcgttga aggcgtctgg
     7801 cagctatggg aacagccgca gaacaccaca gagagggaat tatgagccag tttttttata
     7861 ttcatcctga taacccgcag caacgtctaa tcaaccaggc ggtggagatc gtgcgtaaag
     7921 gcggggtgat tgtttatcca actgattccg gctatgcgct cggctgtaaa attgaagata
     7981 aaaacgccat ggagcgtatt tgtcgtattc gccagctgcc ggacggtcac aactttaccc
     8041 tgatgtgtcg cgatctttct gaactctcga cctattcatt tgtcgataac gttgcgtttc
     8101 gtttaatgaa aaacaacacg ccgggcaact ataccttcat cctgaagggg acgaaggagg
     8161 tgccacgccg tctgttgcag gaaaaacgca aaaccatcgg tatgcgtgtg ccttctaacc
     8221 cgattgcgca ggcgttgctt gaagcactgg gcgaaccgat gctttccact tcgctaatgc
     8281 tgccaggcag cgaatttacc gaatccgatc ccgaagaaat caaagaccgt ctggaaaaac
     8341 aggtcgattt gattattcac ggcggctatc tcgggcagaa accaacaacg gttattgatc
     8401 ttaccgatga tacgccggtc gtggtgcgtg aaggcgtagg tgatgtgaag cctttcttat
     8461 aacaaattgc gttccacgga tggaagacta tggcagggaa gtttcgctgc attttgctgt
     8521 tgatagttgg gctttttttc tcttcgttaa gttatgcgaa aaacacggag atgccttctt
     8581 atgaagaagg gatctcgctc tttgatgttg aagccactct gcaaccggat ggggtgctcg
     8641 acatcaaaga aaatattcat tttcaggcgc gaaatcagca gattaagcac ggattttatc
     8701 gtgatttacc acgactatgg atgcagcctg atggggacgc tgcactgctg aactatcata
     8761 ttgttggcgt cacccgtgat ggtattcctg aaccctggca tcttgactgg catatcgggt
     8821 taatgagtat tgtcgtgggc gataaacaac gtttcttgcc tcaaggcgac tatcattatc
     8881 aaattcatta tcaggttaaa aatgctttcc tgcgtgaggg agattcagat ctgttaatct
     8941 ggaacgtgac tggtaaccac tggccgtttg aaatctataa gacccgattt tcactcaagt
     9001 tccctgatat cgcgggtaat ccatttagcg aaatcgatct ctttactgga gaagagggcg
     9061 acacatatcg aaatggccgc atccttgagg acggaagaat tgaatccagc gatccgtttt
     9121 atcgtgaaga tttcacggta ctctaccgtt ggcctcactc gttacttagc aatgccccgg
     9181 ctccacaaac gacgaatatt ttcagccatc ttcttttacc ctccacgtca tcgttgttaa
     9241 tttggtttcc gtgtctattc ctggtttgtg gatggttata tctctggaag cgcaggccgc
     9301 aatttacgtc ggtagatgtt ttacggatgc agaaatttta tctgccgcgt aaaaagtctt
     9361 cgttttaccg gcctgatact tttttgcaat ggggtggact ggcaatattg gcggtcattc
     9421 tttacggtaa cctgagtcct gtaggctggg caggaatgag tctggtgggc gatatgttta
     9481 ttatgatctg ctggcttctt ccttttttat tttgttccct tgagcttttg tttgcccgcg
     9541 atgatgacaa gccttgcgtt aatcgtgtaa tcatcacttt gtttttaccg ctgatttgtt
     9601 caggcgtggc cttttattct ctctatatca atgtcggaga tgtattcttt tactggtata
     9661 tgccagcggg ttattttagc gctgttttcc tgaccggtta tctcactggc atggggtata
     9721 tttttctgcc aaagtttacc caaactgggc agcaacgtta tgcccacggt gaagctatcg
     9781 ttaactatct cgcgcgtaaa gaggcagcaa cacacagtgg gcgtcggcgg aaaggggaaa
     9841 cacggaaact ggattacgcg ttgctaggtt gggctgtctc agcaaacctt ggaagagaat
     9901 gggtagcacg tatcacccca tcactcacag cggctgttcg cgccccggaa attgcccgta
     9961 gtggcgtttt gttctcatta cagatgcacc tgagtctggg ggccaatacc agtttattgg
    10021 ggcgaagtta ttccggtggt gctgcgggtg gcggaggcgg tggtggctgg taattaagct
    10081 gatgttaatc gaaacaagct ttaaccataa agatagtgta gtatgttgcg cctcaaagcc
    10141 aggccagtta aaacgtcgag tcgtttactt aaggcctgaa gagttcaaac agactttact
    10201 atcagtgagt cagatgtaag ttctgactca ctgttgcaca aaagcatatc agacgcctgg
    10261 gaaggcgaca ccaaaaggaa gctctatgag cgaaaagcta cagaaagtgc tggcgcgtgc
    10321 cggccacggt tctcgccgtg aaatcgaatc tattattgaa gccggtcgtg tgagtgttga
    10381 tggcaaaatt gctaaactcg gcgatcgcgt tgaagttacc cctggattga aaatccgtat
    10441 cgatggtcac ctgatttcgg tacgtgagtc cgctgaacaa atttgtcgcg ttctggccta
    10501 ttataaaccg gaaggtgagt tgtgtacccg taacgatccg gaagggcgtc caacggtgtt
    10561 tgaccgtctg ccaaagctgc gcggtgcacg ctggattgcc gtaggtcgtc tggacgttaa
    10621 cacctgtggt ctgttgctgt tcaccaccga cggtgaactg gctaaccgtt taatgcatcc
    10681 aagccgtgaa gttgaacgtg aatatgccgt gcgtgtattt ggtcaggttg acgacgcgaa
    10741 actgcgtgat ttaagccgtg gtgtgcagct ggaagatggt ccggcagcct ttaaaaccat
    10801 caagttcagc ggcggcgaag ggatcaacca gtggtacaac gtgactctga ccgaaggccg
    10861 taaccgtgaa gttcgtcgtc tgtgggaagc ggttggcgtg caggtaagcc gcctgatccg
    10921 tgttcgttac ggtgatatcc cactgccgaa aggtctgcca cgcggcggct ggacggagct
    10981 ggatcttgcc cagactaact atctgcgcga actggtggag ttgccgccag aaaccagctc
    11041 taaagtcgct gtagaaaaag accgtcgtcg catgaaggcg aatcagattc gtcgtgcggt
    11101 gaaacgtcac agtcaggtga gcggcggacg tcgttctggt gggcgtaata acggttaatc
    11161 aacgtatgcc ggacgtcata tccggcattt ttacagatta gtaatcaatc cctatctgcg
    11221 cttttacgcc agcatcaaac gcatgtttca cggggcgtaa ctcacttacc gtatccgcca
    11281 gttcgagtat atcccgatga cagccccgac ccgtgataat caccgtctgc tgatgtggac
    11341 gttcatttaa cgcctgcaac acttcttcca gtggcaaata gtcatacgcc accatataag
    11401 tcagctcatc aagcaaaacc aaatccagtg aggaatcagc aagcatccgc tttgcatgtt
    11461 gccagacttc gcggcaggcg gcggtatcag actcgcggtt ttgtgtatcc caggtaaagc
    11521 ccgttgccat cacctgaaac tcaacgccat gtggctccag cagattgcgt tcgccattag
    11581 gccaggtgcc tttaataaac tgcacgacgc ccactttttt tccgtgacca actgcgcgtg
    11641 ttgccgtacc aaatgccgcg gtggttttgc cttttccatt gccggtaaag acgatgataa
    11701 taccgcgttc atcctgggcc tgggccatac gagcatctac tttttctttc actcgctgct
    11761 gacgctgttg gtagcgttca tcactcattg ggaaattcct ggtttacggc ccggttgggc
    11821 gtcaaaggtc atgccggttt tacggcggct gtcatcgccc atcagccaga ggtagagcgg
    11881 catgatatca gcgggtgttt taagtttctg tggatcttcg gtcgggaagg cgctggcacg
    11941 cattgcggtg cgcgtaccgc ctgggttaat gcagttgaca cgcaggcgct gctgatattc
    12001 atcggccagt acctgcatca tcccttcggt ggcaaatttc gacgctgcat atgcacccca
    12061 gttggctcgt ccctgacgtc caacgcttga tgaagtaaag accagtgaac cggcgtccga
    12121 tttgagtaat aaaggaagta gtgcctgggt gagcataaag gtggcattaa cgttgacctg
    12181 tatgacgtcc tgccagacct gtggattttg ttcgctcatt ggacaaacat cgccgagtaa
    12241 tccggcatta tgcaaaacac catccagacg cggataatta acggcaatgc gctgtgccag
    12301 ttgttggcaa tcttcggacc tgcaggtcag caaatcgaga ataaaccact gtggctgacg
    12361 cccagtttct tcgtttatgt ggctggctac ctgacgtaat ttttcttcat tacggcccaa
    12421 cagaatcact gtcgcaccat agcgtgcata cgtcatcgcg gcttcacgac caataccatc
    12481 actggctccc gtcaccagga taatgcgatc attgagtaaa tcttgttttg gctggtaatg
    12541 catggctact cctcaacgac gttgtctgtc gcgctgccag tgtactttat gactttgggg
    12601 ctttatgcct gaaacagaag ggcatttcaa tcagcctcgg gcgataacac cgcaaaatgg
    12661 atactttgtc atactttcgc tgcaataaca tctctgcgag acggcttaac atgcctgttg
    12721 taaactgtga gccaaagcgt tgtttaacca aggtggggac tcgtggaatt gttgtctgaa
    12781 tatggtttgt ttttggcgaa aatcgttacc gttgtgctag cgattgcggc gattgccgcc
    12841 attattgtca atgttgctca acgtaataaa cgccagcgtg gcgagttacg ggtcaacaat
    12901 ctcagcgaac agtataagga gatgaaagaa gaactggccg cggcgctgat ggactcacat
    12961 cagcaaaaac agtggcacaa agcgcagaag aaaaagcaca agcaagaagc gaaagcagca
    13021 aaagcgaaag ccaaactggg cgaggtgtca actgacagta aaccccgcgt ctgggtgctg
    13081 gactttaaag gcagcatgga cgcccatgaa gtgaactcgc tacgtgaaga gataacggcg
    13141 gtacttgcag cattcaaacc gcaggatcag gttgtgctcc gtctggaaag ccctggtggc
    13201 atggtgcatg gttacgggtt ggcggcttcg cagctgcagc gtctgcgtga taaaaacatt
    13261 ccattaactg ttacggtaga caaagttgct gccagcggtg gttacatgat ggcctgtgtg
    13321 gcggacaaaa ttgtttccgc accgtttgct attgtgggtt ccattggggt ggtggcgcaa
    13381 atgcccaact ttaaccgctt cctgaaaagc aaagatattg atatcgaact gcacaccgcc
    13441 gggcagtata agcgtacgct gaccttgctg ggtgaaaata ccgaagaagg gcgggagaaa
    13501 ttccgcgaag agctgaacga aacgcatcag ttatttaaag attttgtgaa gcgtatgcgt
    13561 ccgtctctgg atattgaaca ggtggcaacg ggtgaacact ggtacggaca acaggcggta
    13621 gagaagggcc tggttgatga aatcaacacc agtgatgaag ttattcttag cctgatggaa
    13681 ggccgagaag tggtcaatgt acgctatatg cagcgtaaac gactcattga ccgattcacc
    13741 ggcagcgcgg cagagagcgc cgatcgatta ttgctacgct ggtggcagcg tgggcaaaag
    13801 ccattgatgt aaaagacaaa cgcgagggta agacctcgcg ttttgcttta atcaaccaga
    13861 tgatattttt ctgaaagcac atgggccagg tgtttgaaca tattaaacac cgcggtgctt
    13921 ttggctgttg gcaatccttg ttcatctaaa aagtagtcgc cggtaaatac cagcacgcca
    13981 ttacgctgtg tgacgccggt ggcttcaatc cctgcgagcg tatcctcatg ctcacgaatg
    14041 attttgttgg cctctttcaa cagcgtttcg cggtcgatgg gttgtgtctc tttgttcatt
    14101 atttactcct taaacaagga cattagtcta cgccaggcat ggcttgcaga caaatatacc
    14161 acgctggtgg caagagcgcc ttactggcaa ctttggattt tgcatgctaa taaagttgcg
    14221 tatcggattt tatcaggtac agtgtgacgc tttcgtcaat ctggcaatag atttgcttga
    14281 cattcgacca aaatttcgtc gtgctatagc gcctgtaggc caagacctgt taactcagtc
    14341 acctgaattt tcgtgaacag agtcacgaca aggggttgat atccgcagag agcgagtcca
    14401 tatcagtaac tcgttgccag tggaaggttt atcaacgtgc gacgcattcc tggaagaatc
    14461 aaattaggta aggtgaatat gggtaaagct cttgtcatcg ttgagtcccc ggcaaaagcc
    14521 aaaacgatca acaagtatct gggtagtgac tacgtggtga aatccagcgt cggtcacatc
    14581 cgcgatttgc cgaccagtgg ctcagctgcc aaaaagagtg ccgactctac ctccaccaag
    14641 acggctaaaa agcctaaaaa ggatgaacgt ggcgctctcg tcaaccgtat gggggttgac
    14701 ccgtggcaca attgggaggc gcactatgaa gtgttgcctg gtaaagagaa ggtcgtctct
    14761 gaactgaaac aactggctga aaaagccgac cacatctatc tcgcaaccga ccttgaccgc
    14821 gaaggggaag ccattgcatg gcacctgcgg gaagtgattg gtggtgatga tgcgcgctat
    14881 agccgagtgg tgtttaacga aattactaaa aacgcgatcc gccaggcatt taacaaaccg
    14941 ggtgagctga atattgatcg tgttaatgcc cagcaggcgc gtcgctttat ggaccgcgtg
    15001 gtggggtata tggtttcgcc gctgctatgg aaaaagatcg ctcgtggtct gtctgccggt
    15061 cgtgtgcagt cggtggcagt ccgcctggtg gtcgagcgtg agcgtgaaat taaagcgttc
    15121 gtgccggaag agttctggga agtcgatgcc agcacgacca cgccatctgg tgaagcgttg
    15181 gcgttgcagg tgactcatca gaacgacaaa ccgttccgtc cggtcaacaa agaacaaact
    15241 caggctgcgg taagtctgct ggaaaaagcg cgctacagcg tgctggaacg tgaagacaaa
    15301 ccgacaacca gtaaacctgg cgctcctttt attacctcta cgctgcaaca agctgccagc
    15361 acccgtcttg gatttggcgt gaaaaaaacc atgatgatgg cgcagcgttt gtatgaagca
    15421 ggctatatca cttacatgcg taccgactcc actaacctga gtcaggacgc ggtaaatatg
    15481 gttcgcggtt atatcagcga taattttggt aagaaatatc tgccggaaag tccgaatcag
    15541 tacgccagca aagaaaactc acaggaagcg cacgaagcga ttcgtccttc tgacgtcaat
    15601 gtgatggcgg aatcgctgaa ggatatggaa gcagatgcgc agaaactgta ccagttaatc
    15661 tggcgtcagt tcgttgcctg ccagatgacc ccagcgaaat atgactccac gacgctgacc
    15721 gttggtgcgg gcgatttccg cctgaaagca cgcggtcgta ttttgcgctt tgatggctgg
    15781 acgaaagtga tgcctgcact gcgtaaaggc gatgaagatc gtatcttacc tgcagtcgat
    15841 aaaggcgatg ctctgacgct cgttgaactg acaccagccc agcactttac caagccgcca
    15901 gcccgtttca gtgaagcatc gctggttaaa gaactggaaa aacgtggtat cggtcgtccg
    15961 tctacctatg cgtcgatcat ttcgaccatt caggatcgtg gctatgtgcg agtagaaaat
    16021 cgtcgtttct atgcggaaaa aatgggcgaa atcgtcaccg atcgcctgga agagaatttc
    16081 cgcgagttaa tgaactacga cttcaccgcg cagatggaaa acagccttga ccaggtggca
    16141 aatcacgaag cagagtggaa agctgtactg gatcacttct tctcggattt cactcagcag
    16201 ttagataaag ctgaaaaaga tccggaagag gggggtatgc gtccgaacca gatggttctg
    16261 accagcatcg actgcccgac ctgtggtcgc aaaatgggga ttcgcacagc gagcaccggg
    16321 gtattccttg gctgttctgg ctatgcgctg ccgccgaaag agcgttgcaa aacaaccatt
    16381 aacctggtgc cggaaaacga agtgctgaac gtgctggaag gcgaagacgc tgaaaccaac
    16441 gcgctgcgcg caaaacgtcg ttgccccaaa tgcggcacgg cgatggacag ctatctcatc
    16501 gatccgaaac gtaagttgca tgtctgtggt aataacccaa cctgcgacgg ttacgagatc
    16561 gaagagggcg aattccgcat taaaggttat gacggcccga tcgttgagtg tgaaaaatgt
    16621 ggttctgaaa tgcacctgaa aatggggcgt ttcggtaaat atatggcctg caccaacgaa
    16681 gagtgtaaaa acacgcgtaa gattttacgt aacggcgaag tggctccacc gaaagaagat
    16741 ccggtaccat taccggagct gccgtgcgaa aaatcagatg cctatttcgt gctgcgtgac
    16801 ggtgctgccg gtgtgttcct ggcggccaat accttcccga aatcgcgtga aacgcgtgcg
    16861 ccgctggtgg aagagctgta tcgcttccgc gatcgtctgc cggaaaaact gcgttatctg
    16921 gccgatgcgc cgcagcagga tccggaaggt aataagacta tggttcgctt tagccgtaaa
    16981 accaaacagc aatatgtctc ttcggaaaaa gacggaaagg cgactggctg gtcagcattt
    17041 tatgttgatg gcaaatgggt tgaagggaaa aaataacctt taattctgtc aggtttttat
    17101 aaacaaaggg tcgcgaaagc ggcccttttt tattgcatat tattttttct tcacacctat
    17161 acactaaggc tataaatgat atagtggtta tagttagcac cttttttatt attaaatcgt
    17221 attagtcacc cgccaggtgt gacgaaaaaa cgatgttctg atggcgtcta agtggatggt
    17281 ttaacatgaa attacaacaa cttcgctata ttgttgaggt ggtcaatcat aacctgaatg
    17341 tctcatcaac agcggaagga ctttacacat cacaacccgg gatcagtaaa caagtcagaa
    17401 tgctggaaga cgagctaggc attcaaattt tttcccgaag cggcaagcac ctgacgcagg
    17461 taacgccagc aggacaagaa ataattcgta tcgctcgcga agtcctgtcg aaagttgatg
    17521 ccataaaatc ggttgccggt gagcacacct ggccggataa aggctcgctg tatatcgcca
    17581 ccacgcatac ccaggcacgc tacgcgttac caaacgtcat caaaggcttt attgagcgtt
    17641 atccccgcgt ttctttgcat atgcaccagg gttcgccgac acaaattgct gatgccgtct
    17701 ctaaaggtaa tgcagatttc gctatcgcca cagaagcgct gcatctgtat gaagatttag
    17761 tgatgttacc gtgctaccac tggaatcggg ctattgtagt cactccggat cacccgctgg
    17821 caggcaaaaa agccattacc attgaagaac tggcgcaata tccgttggtg acatatacct
    17881 tcggctttac tggacgttca gaactggata ctgcctttaa tcgcgcaggg ttaacgccgc
    17941 gtatcgtttt cacggcaacg gatgctgacg tcattaaaac ttacgtccgg ttagggctgg
    18001 gggtaggggt cattgccagc atggcggtgg atccggtcgc cgatccggac cttgtgcgtg
    18061 ttgatgctca cgatatcttc agccacagta caaccaaaat tggttttcgc cgtagtactt
    18121 ttttgcgcag ttatatgtat gatttcattc agcgttttgc accgcattta acgcgtgatg
    18181 tcgttgatgc ggctgtcgca ttgcgctcca atgaagaaat tgaggtcatg tttaaagata
    18241 taaaactgcc ggaaaaataa tttcttgcgt tattttcggc accttttatg tagcgaaggt
    18301 gccggaatat attctc
//
LOCUS       DAZKIP010000087        18059 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZKIP010000087 DAZKIP010000000
VERSION     DAZKIP010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18059
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.087"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(314..1906)
                     /gene="lsrK"
                     /locus_tag="ACHOUK_003249"
     CDS             complement(314..1906)
                     /gene="lsrK"
                     /locus_tag="ACHOUK_003249"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310145.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HFP9898639.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTIRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKVGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSTIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(1985..2938)
                     /gene="lsrR"
                     /locus_tag="ACHOUK_003250"
     CDS             complement(1985..2938)
                     /gene="lsrR"
                     /locus_tag="ACHOUK_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFP9898640.1"
                     /translation="MTINDSVISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            3187..4722
                     /gene="lsrA"
                     /locus_tag="ACHOUK_003251"
     CDS             3187..4722
                     /gene="lsrA"
                     /locus_tag="ACHOUK_003251"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFP9898641.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITSADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFTRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAAGTSVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNDKEINRLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLELYRRALNI
                     KYNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     AAC"
     gene            4716..5756
                     /gene="lsrC"
                     /locus_tag="ACHOUK_003252"
     CDS             4716..5756
                     /gene="lsrC"
                     /locus_tag="ACHOUK_003252"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFP9898642.1"
                     /translation="MLKFIQNNREITALLAVLLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAVLGAWFL
                     TQIDSVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALELNLRRQKYARFMTPPPSV
                     KPASSGKKREAA"
     gene            5756..6748
                     /gene="lsrD"
                     /locus_tag="ACHOUK_003253"
     CDS             5756..6748
                     /gene="lsrD"
                     /locus_tag="ACHOUK_003253"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFP9898643.1"
                     /translation="MRIRYGWELALAALLVIEIVSFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGYPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGAIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6760..7782
                     /gene="lsrB"
                     /locus_tag="ACHOUK_003254"
     CDS             6760..7782
                     /gene="lsrB"
                     /locus_tag="ACHOUK_003254"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HFP9898644.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7809..8684
                     /gene="lsrF"
                     /locus_tag="ACHOUK_003255"
     CDS             7809..8684
                     /gene="lsrF"
                     /locus_tag="ACHOUK_003255"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFP9898645.1"
                     /translation="MADLDDIKDGKDFRTDQPQKNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGTQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            8708..8998
                     /gene="lsrG"
                     /locus_tag="ACHOUK_003256"
     CDS             8708..8998
                     /gene="lsrG"
                     /locus_tag="ACHOUK_003256"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFP9898646.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            9055..9813
                     /gene="tam"
                     /locus_tag="ACHOUK_003257"
     CDS             9055..9813
                     /gene="tam"
                     /locus_tag="ACHOUK_003257"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFP9898647.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENVDYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGRESLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLKEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(9817..10731)
                     /gene="yneE"
                     /locus_tag="ACHOUK_003258"
     CDS             complement(9817..10731)
                     /gene="yneE"
                     /locus_tag="ACHOUK_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFP9898648.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQVEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKVLPDRHYQLT"
     gene            complement(10928..12379)
                     /gene="uxaB"
                     /locus_tag="ACHOUK_003259"
     CDS             complement(10928..12379)
                     /gene="uxaB"
                     /locus_tag="ACHOUK_003259"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFP9898649.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(12606..14024)
                     /locus_tag="ACHOUK_003260"
     CDS             complement(12606..14024)
                     /locus_tag="ACHOUK_003260"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFP9898650.1"
                     /translation="MHVHPISTFRVFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPQMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(14163..14522)
                     /gene="yneG"
                     /locus_tag="ACHOUK_003261"
     CDS             complement(14163..14522)
                     /gene="yneG"
                     /locus_tag="ACHOUK_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFP9898651.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPHGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(14522..15448)
                     /gene="glsB"
                     /locus_tag="ACHOUK_003262"
     CDS             complement(14522..15448)
                     /gene="glsB"
                     /locus_tag="ACHOUK_003262"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFP9898652.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(15512..16900)
                     /gene="sad"
                     /locus_tag="ACHOUK_003263"
     CDS             complement(15512..16900)
                     /gene="sad"
                     /locus_tag="ACHOUK_003263"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFP9898653.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGADDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVTQMIKDSRIAAITVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMDDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITIAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            17001..17882
                     /gene="yneJ"
                     /locus_tag="ACHOUK_003264"
     CDS             17001..17882
                     /gene="yneJ"
                     /locus_tag="ACHOUK_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9898654.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAIVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWCWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSTKQGYQ"
     gene            17960..>18059
                     /gene="yneK"
                     /locus_tag="ACHOUK_003265"
     CDS             17960..>18059
                     /gene="yneK"
                     /locus_tag="ACHOUK_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFP9898655.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDF"
BASE COUNT         4537 a         4588 c         4493 g         4441 t
ORIGIN      
        1 taagaaaact gtttctttca gtaggaaaat tatcatcatc atatttaaca aagaatagca
       61 ccaattgcta aaaatcgaag tttattaaac cccttttgca tccattaatg tctttaggaa
      121 tattcgttat aaaataaggg ttatgcttaa tgctttaaga aaaatagcaa tttccccttg
      181 aatatagtac tggtgatgaa acgatgaatc tgcaagctgg catttaacaa gccagctcta
      241 aaagaaggga aataagaata actatactca aaaactaaca gccacggtca tcatgatgtg
      301 gctgtcaatg aaactataac ccaggcgctt tccataacga cgtcgtcagt ccatgatcaa
      361 ccagccccag ctgatcctga taaactgcct gccacttatc gcgtgaatcc tgataaagtt
      421 catgcttttc cgggtctggt gtgtgcgtcc gttcccagcg aaccaggcgt tctccggttt
      481 ctgccattga tgaaaaaatt ccggcaccga cgccagctgc aatggcacat cctaatgcag
      541 tggcttcttt gaccaccgga atattgacgg gtaatcccga gacatcagcg agaatttgac
      601 tccataattt cccttttgaa cctccgcctg caaagactaa cgatgaagga tgaatagtcg
      661 aaaaatcagc aatttgctgc aagttacacg ctgatacaat cgccgcattt tcttccagcg
      721 cacggaacaa tgtcgctttg ttacatttat ccggatcaat ggacaaatta ataaaggaag
      781 gcgcagcgtg ataccaggtt ttaaagcgca ttctgtcgga gaagatcggc attacgcccc
      841 acgacccagg cggcacccga ctggccatct cttccagcag cgtataggtg tcgatgccta
      901 aacgttcggc aatcagtttt tcttcggcac agaaagcatc gcggaaccag cgcatggtga
      961 gtccggtaaa aaagcttata gattcagctt gtaccatgcc aggaataaca tgaggattaa
     1021 cgcgcacgtt catttctggg tctgtcaccg gcgcggctaa atttacaact tgctgccaga
     1081 atgtgccgcc aagaaccgcg gtttgtgccg gacgcacaac gcctaacccc aggcaaccaa
     1141 gctgcacgtc gcctcctcca acgaccaccg gagtgcccac cttcagaccg cagagttccg
     1201 ccgcttgtga acttaccacg cccagcaatg tgccggtttc tttgacagga gaaagaatat
     1261 cggcacgcag gccagccata tccagcaatg caggtttcca gtcacggatg gttagatcaa
     1321 gaagtcccgt ggtgccagcg ttagagggat ccaccgcaag ttcgccgctg agcatatagg
     1381 ccagccagtc gctgatcatg gtgatggttg atgcctgacg gtaaatatcg gaacgatggt
     1441 gcgccagcca aagtaatctg gggatggcac ttaaagccag tgtttgtccg gtcgcgcgat
     1501 aaacttcgtt ttcaaaggta ttgttgtgca gttctttaag ttcgctaact tcgcgcgccg
     1561 ctctggcatc cacattggcg caggcccaga tcggtgttcc ttcattgtta tataaaacaa
     1621 tgccttcacg catcgaacat gccgaaacgg cagcgatata ctccggggct atgccggcgt
     1681 tgtgcagcgc ctggcgcata cactcacacg ccagttgcca gtttttgttg agatcaaatt
     1741 ccatagaacc aggaacgtcc ggtactgcca gatgccgcca ctccgcctgt cccactgcta
     1801 tttgattgcc ttccaggtcg aatatcacag cccgaatact tccggtgcct gcatccagcg
     1861 ccatcaggta gtactttgat tctgaagggg taaagagtcg agccattaga ttatcctcgg
     1921 cttatgcgtt ctgtctgttc ctctatacgt tctccatcat tcccggtaat aaggtcatgc
     1981 aaatttaact acgtaaaatc gccgctgctg tgtcctgatc ggtaaccagt gcgttgatat
     2041 aaccgccttt cattgcagcg gcaattgctt cggctttatt ttctccccct gccacgccaa
     2101 cccggacggg tatggtcttc agcgcgctta aaggtaagcc aatcagctcg ttatgtattt
     2161 taatgtccgt gacaacgtca ccttttgcat caaaaaagta gcctaaaatg tcgccaaccg
     2221 ccccttttcg gccaatcatt aactgttcgc cctggctgat ataaccggag cgaatgatcg
     2281 tcgcgtcgtc ttgttgacta acggccccaa tgccgacaat cgccacatcc gctgcttgcg
     2341 cggctaacag aacatctttg acacaatttt cattttttag cgtacgggca atgtcagcgg
     2401 aggatgcccg caacggagcc ggaataatat tcacactgca cgccgcatta agctgtccga
     2461 tacccgtcat ataagaaccg acgccaccgg agagcgtgac caggcgaatt tgctgtgacg
     2521 aaataaaacc acttaagcgt tgcagcgtat tcatggttgc ctcgccaaaa ccaatcgcca
     2581 gcatctgttg tggttgaagt aaactcatca acatatgcgc cgcgcctatc cccagtcgcc
     2641 cgccgacatc agcatccgca agcccaggga tcacccggac atgttgcagc gaaaactgac
     2701 gacgtaattg tgtttcatat tccagacagc cttcaaagcg agaattaatc tgcacgcgaa
     2761 tgatgccgga ctgatgccct ttctccagta atcgcgacac tttcaaacgg gtcaggccga
     2821 gacgatcgct gatctcactc tgggtcagcc cgtcgtggta gtaaaaccac gcgatccgcg
     2881 cgacctgttc ttcttcacac attccctgtt ctgaaattac cgaatcgttg attgtcataa
     2941 ttcattcttc actttgaaca tatttaaatc tttaatgcaa ttgttcagtt cttgcgcatt
     3001 tatatctgtg atggcaacca cagtttgact ctacgagcat gaacaaacgc aaccgtgaaa
     3061 atcaaaatag tataaattgt gatctattcg tcggaaatat gtgcaatgtc cacctaaggt
     3121 tatgaacaaa ttaaaagcag aaatacattt gttcaaaact cacctgcaaa actgaacggg
     3181 ggaaatatgc aaacgagtga tacccgcgcg ttaccgctac tttgcgcccg ctcggtttat
     3241 aaacagtatt caggggtcaa tgtcctgaaa ggcatcgatt ttacgttgca tcagggggag
     3301 gtccacgccc tgctcggcgg caatggtgcc ggtaaatcga cgttaatgaa gattattgcc
     3361 ggtattacct ctgctgatag cggtacgctg gagattgggg gcaacaacta cgccagatta
     3421 acgccagttc atgctcatca gctgggtatt tatctcgttc cccaggaacc gctgcttttc
     3481 ccaagcctgt cgataaaaga aaacatcctg tttgggctgg caaaaaaaca gctctccatg
     3541 cagaaaatga agaacttgct ggcggcgctg ggctgccagt ttgatctgca tagtctggca
     3601 ggatcgctgg atgtcgccga tcgccaaatg gtggaaatcc tccgcgggct gatgcgcgac
     3661 tcgcggattc tgatcctcga tgaacctacc gcctcgctta cccctgcgga aaccgaacgc
     3721 ttgtttactc gcttgcaaga gctgcttgct actggcgtgg gtattgtttt tatctcgcat
     3781 aagctgccgg aaattcgcca gattgccgat cggattagcg tgatgcgcga cggaaccatc
     3841 gccttaagcg gcaaaaccag cgaactgtct accgacgaca ttattcaggc catcacccca
     3901 gcggtacggg aaaaatcgct ctctgccagc caaaaattat ggctggagtt acctggcaac
     3961 cgcccacaac atgccgcagg aacgtcggtg ctgacactgg aaaatctgac tggcgaaggt
     4021 ttcaggaatg tcagcctgac gctaaatgcc ggagaaattc tgggtctggc tgggctggtg
     4081 ggtgccggac gcacagaact ggccgagacg ctctatggtc tacgtacttt gcgtggcgga
     4141 cgcattatgc tgaatgataa agagatcaat agattatcca ctggagaacg tttactgcgc
     4201 ggtctggttt atctgccgga agatcgccag tcatccggac tgaatctcga tgcttcgctg
     4261 gcctggaacg tctgcgccct tactcataac cttcgtggat tctgggcgaa aaccgcgaaa
     4321 gataatgcca ccctggaact ttatcgtcgg gcgctgaata ttaaatataa ccaaccggaa
     4381 caagctgcac ggacattatc cggtggtaac cagcaaaaaa tcctgattgc caaatgcctg
     4441 gaagcctcgc cgcaagtatt gattgtcgat gagccgacgc gcggcgtgga tgtctcggcg
     4501 cgtaatgata tctaccaact gttgcgcagc atcgccgcac agaatgtggc tgtgctgctt
     4561 atctcctctg atctggaaga gatcgaactg atggcagatc gtgtgtatgt gatgcatcag
     4621 ggcgaaatta cccactctgc actgaccggg cgcgatatta atgtcgagac tattatgcgc
     4681 gttgccttcg gcgatagtca gcgtcaggag gcggcatgct gaagtttatt cagaacaacc
     4741 gtgaaatcac ggcactgctg gcggtgctgc tgctgtttgt attacccggt tttctcgacc
     4801 gccagtattt aagtgtgcaa acgctgacca tggtttatag cagcgcgcaa atcctgatcc
     4861 tgctggcaat gggcgcgacg ctggtaatgc ttacgcgcaa tattgatgtt tcagtgggtt
     4921 cgattaccgg aatgtgcgcg gtgctgttgg ggatgttact gaacgcagga tattcactac
     4981 ctgttgcctg tgtcgcgact ttactgcttg gtttgctcgc gggatttttc aacggtgtcc
     5041 tggtcgcgtg gctaaagatc cctgccattg ttgccaccct tggcacgtta gggttgtaca
     5101 gaggcatcat gttgctgtgg actggcggta aatggattga agggttaccc gccgaactga
     5161 aacagctctc cgccccgctg ctgcttggcg tttcagcaat tggttggtta acgataattc
     5221 tggtggcatt tatggcctgg ctgctggcga agacagcgtt tggacgcagt ttttatgcca
     5281 cgggcgataa tttacagggc gctcgtcaac tgggcgttcg tacggaagcc attcgcattg
     5341 tggcattttc gttgaacggc tgcatggcgg cactggcggg aattgtgttt gcttcgcaga
     5401 ttggttttat ccccaaccag accggtaccg ggctggagat gaaagcaatt gcagcctgcg
     5461 tgctgggcgg cattagtttg ctcggcggtt ccggtgcgat catcggtgcg gtactcggtg
     5521 cggtactcgg cgcatggttc ctgacgcaga tcgatagcgt actggtgctg ttgcgcattc
     5581 cggcatggtg gaatgatttt atcgcgggtc tggttctgct ggcggtgctg gtgtttgatg
     5641 gacgcctgcg ttgtgcgctg gaacttaatc tgcggcggca aaaatatgcc cgctttatga
     5701 cgccaccgcc atccgttaaa cccgcttcgt caggtaaaaa acgggaggct gcataatgcg
     5761 tattcgctac ggttgggaac tggctcttgc cgcactgctt gttattgaga ttgtctcatt
     5821 tggtgcaatt aacccgcgga tgttagatct caatatgttg ctgttcagca ccagtgactt
     5881 tatctgcatt ggcattgtcg ccctaccgct gacgatggtg attgtcagtg gcgggatcga
     5941 tatttcgttt ggttcgacca tcggcctctg cgccattgca ttgggcgtac tgtttcaaag
     6001 tggtgtgccg atgccgctgg cgattctcct gaccttactg ctcggcgcat tgtgcgggct
     6061 gatcaacgcc ggattaatta tctataccaa agttaacccg ctggtgatta cgcttggcac
     6121 gctgtatctg tttgccggaa gcgccctgct gctttccggg atggccgggg cgacggggta
     6181 cgaaggtatt ggtggatacc cgatggcgtt tacagatttc gctaacctgg atgtgttggg
     6241 gctccccgtt ccgctgatta tcttcctgat atgtctcctc gttttctggc tctggctgca
     6301 taaaacccat gccggacgta acgtgttttt gattgggcaa agcccgcgcg tggcgcttta
     6361 tagcgcgatt ccagttaacc gtaccttatg tgcgctctat gccatgacgg ggctggcgtc
     6421 tgcggtcgcc gctgtgctgc tggtgtcgta ttttggttca gcacgttccg atctcggtgc
     6481 gtcgtttctg atgcccgcca tcaccgccgt ggtgcttggc ggtgccaata tttatggtgg
     6541 ttccggtgcc attatcggca ccgccattgc ggttttatta gtgggatatt tgcaacaagg
     6601 tttgcaaatg gcaggagtgc caaatcaggt gtccagcgcc ctttccggtg cgctacttat
     6661 cgtcgttgtc gtaggtcgtt ccgttagcct gcatcgccag caaattaaag agtggctggc
     6721 gcgtcgggcc aataacccat tgccataaag gatatcttca tgacacttca tcgctttaag
     6781 aaaatcgcct tacttagcgc tcttggcatt gccgcaatct ctatgaatgt gcaggccgca
     6841 gagcgtattg catttattcc caaactggtt ggagtgggat tttttaccag cggtggcaac
     6901 ggcgcacaac aagcgggtaa agagctgggc gttgatgtga cctacgacgg gccgacagaa
     6961 cccagtgttt ctggtcaggt acagttgatt aataacttcg tcaatcaagg ttataacgcc
     7021 attatcgttt ctgcggtttc gcctgatggc ttgtgtccag cactgaaacg cgccatgcaa
     7081 cgtggtgtga gagtgctgac ctgggactct gatactaaac cggagtgccg ctcttactac
     7141 attaatcagg gaactcccgc ccagttggga ggtatgttgg tggatatggc ggcgcgtcag
     7201 gtgaataaag acaaagccaa agtcgcgttt ttctactcaa gccccaccgt tacggaccaa
     7261 aaccagtggg tgaaagaagc gaaagcgaaa atcgccaaag agcatcctgg ctgggaaatt
     7321 gtcactacgc agtttggcta taacgatgcc actaaatcat tacaaaccgc agaaggaata
     7381 ttaaaagcgt atagcgatct cgacgccatt atcgcccccg atgccaatgc cctgcccgct
     7441 gccgcacaag ccgcagaaaa cctgaaaaat gacaaagttg cgattgtcgg attcagtacg
     7501 ccaaacgtga tgcgtccata tgtggaacgc ggcacggtga aagaatttgg cctgtgggat
     7561 gtggttcagc aaggcaaaat ttcagtttat gtcgcggatg cattattgaa aaaaggatca
     7621 atgaaaacgg gcgacaagct ggatattcag ggcgtaggtc aggtggaagt ctcgccaaac
     7681 agcgtccagg gctatgacta cgaagcggat ggtaatggca tcgtactgtt accggagcgc
     7741 gtgatattca acaaagagaa tatcggcaaa tacgatttct gatgtgcatt acttaaccgg
     7801 agtaagttat ggcagattta gacgatatta aagatggtaa agattttcgt accgatcaac
     7861 cgcaaaaaaa tatccctttt accctgaaag gttgcggtgc gctggattgg ggaatgcagt
     7921 cacgcttatc gcggatattt aatccgaaaa cgggtaaaac cgtgatgctg gcttttgacc
     7981 atggttattt tcagggacca actaccggac ttgaacgcat tgatataaat atcgccccgc
     8041 tgtttgaaca tgccgatgta ttaatgtgta cgcgcgggat tttgcgcagc gtagttcccc
     8101 cggcgaccaa taagccggtg gtactgcggg cgtcaggtgc gaactctatc ctggcggaat
     8161 taagcaatga agccgtggcg ttatcgatgg atgacgccgt gcgcctgaac agttgcgcgg
     8221 tggcggcgca ggtttatatc ggcagcgaat atgaacatca gtcgatcaaa aatattattc
     8281 agctggtcga tgccggaatg aaagtgggaa tgccgaccat ggccgtgact ggcgtgggca
     8341 aagacatggt gcgcgatcag cgttatttct cgctcgcgac tcgaatcgcc gctgaaatgg
     8401 ggacgcaaat tatcaaaacc tattatgtcg aaaaaggttt tgaacggatt gttgccggat
     8461 gtccggtacc cattgttatt gctggcggta aaaaattacc ggagcgcgag gcgctggaaa
     8521 tgtgctggca ggctatcgat cagggcgctt ctggtgtgga tatggggcgt aatattttcc
     8581 agtctgacca tccggtggcg atgatgaaag ccgtacaggc ggtggttcac cataacgaaa
     8641 cggctgatcg ggcatatgaa ctctatctga gtgaaaaaca gtaactgcgg atttaaggag
     8701 aagaattatg cacgtcacac tggttgaaat taacgttcat gaagacaagg ttgacgagtt
     8761 tatcgaagtt tttcgccaga accacctggg ctctgtacag gaagaaggca atttgcgctt
     8821 cgatgtctta caggacccgg aagtgaattc gcgcttttat atctacgaag cctataaaga
     8881 tgaagacgca gtggcgttcc ataaaaccac gccccactac aaaacctgtg tcgcgaaact
     8941 ggaatcttta atgaccgggc cgcgtaaaaa acgtctgttc aatgggttga tgccgtgagg
     9001 cgaatttatc agttttatct acaattgggg taacgcgctg acgggagtaa ataaatgtct
     9061 gactggaacc cctctttata tctgcacttt gccgctgaac gatcgcgtcc ggcggtggag
     9121 ctgcttgcca gagtgccgct ggagaatgtc gattatgttg ccgatcttgg ctgtggccca
     9181 ggtaacagca ccgccctgct gcatcaacgt tggcctgctg ccaggataac gggcatcgac
     9241 tcgtctccgg cgatgattgc tgaagcgcgc agtgctttgc cagactgcca gttcgtggaa
     9301 gcagacatcc gcaactggca accggaacag gcactcgatc tgatttttgc taatgcctca
     9361 ctgcaatggc tgcccgacca ctacgaattg tttcctcact tggtttcttt acttaatccg
     9421 cagggcgtgc tggcagtaca gatgccagat aactggctgg agccgaccca tgtgctcatg
     9481 cgcgaagttg cctgggaaca aaactacccg gatcgcgggc gtgagtcgtt ggctggtgtt
     9541 catgcttact acgatatttt gagcgaagcc ggatgtgagg tcgatatctg gcgaaccacc
     9601 tactatcacc agatgccgtc gcaccaggcg attatcgatt gggtgaccgc cactggatta
     9661 cgtccgtggt tacaggatct gaccgagagc gaacagcagc tttttcttac gcgctaccat
     9721 cagatgctga aagagcagta tccactgcaa gagaacggac agatactgct ggcatttccg
     9781 cgtctgttta ttgttgcccg ccgtacggag taaatatcac gtcagttggt aatggcgatc
     9841 gggaagaact tttgctggaa tttcggcttc atcgttcatc tgtaagaggt caatttcaat
     9901 agcgttacag atggcatcca gcggtaaatc attgttttca gtaccgaacg gatcttccag
     9961 ttcttccgcc agacaatcca gcgaaataaa agtataggaa atcagcacag agataaaagg
    10021 cgtcatgtaa tgcaggtcca cgaccagcgc gaacggcagc atgatacaaa acagataaac
    10081 ggtacgatgc aaaatcaggg tgtaggcaaa gggaattggc gtgtaggcaa tgcgctcgca
    10141 gcctgccaga accgctgaaa tatcgttaag ccgatcgttg aggctaataa acaggatatc
    10201 tgaaagttgt ccattgcgtc gctgaaccgc caaccactcc cccattatta acaagatgcg
    10261 gttagctgga gagttcgaag ccagtacacg ctgaagatct tcagtcttga gataatgagc
    10321 cagcacttcc acctgtggct gtttgcgtaa tgtcatgcgt aaacagtggg cgaaggcgat
    10381 ttgcagccgg gcaaactccc ttacacttgc cgaatccggc aatgtcgttt ttacctcgcg
    10441 cagtaacgac cgtgaggcaa tcatcaactg cccccaaagt ttgcgcgctt caacgtaacg
    10501 ggcgtaaccg gcattattac gaaaaccaag aaaaatggcg atggcgacac cgagaatgct
    10561 gaacggtgcg agggtgaatt tgatgcccag atgcgtgtac cagggcagca tgaaaataac
    10621 agcgatagaa aagagaaaat tgagtagtaa gcgcgaggat atcttggata atactgagcc
    10681 atgccagaca aaaatacggc gcagccagtg ttgttgtgga cgaacaatca tggttatctt
    10741 caggcgtgga aaagtcgccc tattaaacgt gattacgatc acattctcaa gacactactt
    10801 acaaattacc tacaaaatta caacattaac aggtgctgca tgtggcacct gttaatgata
    10861 aaggcttata ggatgttgta actaatataa tgaaataaga aacccggtcg aaaccgggtt
    10921 cagagtatta gcacaacgga cgtacagctt cgcgcatccc tttttcgaga atcgcatcca
    10981 ggtcattagc cacctgctcg accagaccag gcacttgcgt caggtcctgc tcccagtggt
    11041 ctttttctgc caacacaatc gctaccagtt cctgggtacc gataacacgg tcacgatgct
    11101 gactccagag ttgctgataa cgttccagcc agtgtgcatc gtcctgtacc ggatatgttt
    11161 ctccattacg ctcaccgcga tagaacgcaa tcaatgccgc taatgcgaaa gtaaggcgcg
    11221 ccggaagtgt gccgtttgcc ttctgccctg ccagcagctg cggcaggatg cgggtacgga
    11281 acttggtcat accgttgagc gcgatagaca gcagctgatg cttaatgtac gggttacgga
    11341 aacgcccggt gactgcactg gcgaaagatt ccagttcatc gcgtggcaaa tccagtaccg
    11401 gaataatttc ttcgtagatg gctttttcaa cgaatgcgca aatttcagca tcgttcatcg
    11461 cttcacctac ggtatccagc cctgcctgga acgccaccgg caccaacgcg gtgtgtgcac
    11521 cgttgagtat tgccactttg cgttctttat acggtttaat atcgtcaaca atcagcacgt
    11581 tgagcggata tttgtccaga cgcagctcgg tcgctaagga tttcggtccc tgaatcacaa
    11641 acaagtaaaa gtgttcagcg gtgtcgagga aaccatcgtg ataacccagt tcttcttcca
    11701 gtttagccac ttcatcacgc ggataaccgg taacgatacg gtccaccagc gtagaacaga
    11761 agctgttagc ctgatccagc cattgaataa atgcttctgg cagtgcccac tcttgcgcat
    11821 agcgcagcac cagttcacgc aacgcgtcgc cattgtagtc gatcaactca cacgggatga
    11881 tgatccaacc tttatccagc gcaccgttga aatggctaaa acgttcgaac agcagacggg
    11941 tcagttttgc cggataactt accgctggcg cgtcatcgaa tttatcgccc gcatggtagc
    12001 tgatacccgc ttcggtggtg ttagagaaaa caaagcgcat ttccgggttg tgcgccagtt
    12061 tcaggaattc atcgtattca ctgtagacgc tgatttcacg attaacagag cgaatcagac
    12121 gcgcgtcgct gaccgcttcc cccttctcat tcaggccacg gataatggtg gtgtacagac
    12181 catcctgcgt gctcagtgac ggcgggaatg aagtttcaat cggacgaaca acgaccacac
    12241 cagaattcag atcggtgtgc tcattcagga gatctatttg ccagtcaaca aaggcgcgca
    12301 ggaagttacc ttcaccaaac tgaatgatac gttctggata ctgtgcaccg ggaaaatcgc
    12361 gacgatttag tgttttcaca atgggttccc ttctgattag tcatacaacc tgtttgaatt
    12421 ggtacgacag gttagcaaac tttaatacga cgaacccctg ttttgatcaa ctcctgatga
    12481 ttaatgagca gttttatgag aaaagtgtgg cgcggatcat ggtttaatcg aggaaatacg
    12541 ccttttcctg gatcataaag tggtagaaca cattgcattc aaatcgcgcg gaatgaataa
    12601 agatgtcaga caacttcctc accgtaacgc atagtgctgg tacggttgcg cccatctttc
    12661 ttcgagcgat acagacaggt atcggcttcg accatcaatt tattaaagtc atcggtcagc
    12721 gttcggtacg atgcgcgacc actaccgacg ccaatactta ccgtgagata aagcgttttt
    12781 tgttgccagg tgaatggttg cagttcaacg cctttacgga ttttttccgc cattagcaga
    12841 ccatcgacag gattcactga cggcactgca acagcaaatt cttcgccgcc catgcgcgcc
    12901 accagcccct tatcaccgac aatcttctga atatgccggg caaacacgct taacacttta
    12961 tcgccacatt catgtccata gttatcgtta atacttttga aatagtcgat atcaagtagc
    13021 atgacagtca gatgttgtgt ctgtttcagc gatggacttt tcagcgcctc ataaagaccg
    13081 gaccgtgagt aaacctgagt cagaaaatca aagtcggctc gcagcgcaac ttgcttcatt
    13141 agcgaattga tcgctgccac gctaaaagaa accataattg ggcatatcgc catcgtggca
    13201 ataccgagac gtgcggagaa catttgcgga atggagaacg gcgaaccgac cgagatatca
    13261 atcaccgaat tagccaccag tacgatttcc accgcaccgg tgacaaaggt caacagacat
    13321 gttacctgcg gcgtatagcg cactgcacac cagattaatg caggcagcgg gaacgccaga
    13381 ctccccgctc cgccaatgac taccgaggca ataaccgaca caataagcgc gatggcgggc
    13441 atcatctgtt ctgctttaaa gcgcggcagc actccaggaa tagccaacgt cagcatacaa
    13501 ggcacgatca acacgcccgt tgagaattgc tcactgaacc agtccgcaag caaaggccag
    13561 aaatccagac tatcaatact gaccgaacca atcgccccga caatagcgca taataatgcg
    13621 gcaatcagac agtaattaaa tagccgtaag gcacttactg gctcatattt atttttcccc
    13681 aaacgcttat cccgcacgac cagtaaggcg acggtaacaa taaacatcat attggagaaa
    13741 ttgatagcca gtgaaaccag tccccattcg gtggttatgg catcataaac cagcatcgcc
    13801 acataactga tcgcatagta atgcagtcga ttaagccaga catagcgggc aaacaccccc
    13861 gccattacgc cattcagcgg ccagaaaaga gaaagttcgt gaaccagccg taactctgca
    13921 ccaataaaat agaacagcgt ggttagcgca aaaatagcga tgctattacg tagcagatga
    13981 ccttcctgga acacacggaa agttgaaatg gggtgtacat gcattaatta tctgtccata
    14041 caattgcggg tggcaattat ctcaacgtaa taagtaaatt ctcataaagg atagcatgtt
    14101 cgctgacagc ggcggcaaat aaaggtaccc tgggaatgct attttgtcgt tttccgcaat
    14161 tctcatggct gattcatctg tacaaccagc cagtgataga ttaccgccac gatataacgt
    14221 tgttgttctt cgcttagcga aacaccatgc gggatgttat gccacttagc cagacaaccg
    14281 cgacagcagg tcgcggtggc gtgctgagcg ataaacaccg gatgcccgcg catgggggtt
    14341 tgcttaccgt cattagccgg taatgccgga gcaagacgtt tagcgacaaa atccgccgca
    14401 tgttgttcga tgactggcgc gcctttctcc agacaatact gacgctcttt catgcccaaa
    14461 cgaaagcgag atcgaaattt tgaacgggat aaacgcgcga agagtggatc gagagactgc
    14521 attaataaac cgaacgccct aactgtttcg tcaattgttc aaggacggcg atacccgcaa
    14581 gcgagttacc tgcatcatcc agttccggac tccagactgc gatggccatt tcatgcggaa
    14641 caatcgccac aataccgcca ccaacgccag atttcgccgg tagtcccacc cgccaggcaa
    14701 actcccccgc gttctggtac ataccactgg tcgccatcag cgcgttaatt tgccgtgcct
    14761 gcattggcgt caccacaggt tcatcaatat gaatagcttt cccctgatta gccagaaaga
    14821 caaacgtccg ggccagctct acacagctca ttttcagagc gcagtaatga aaatagtttt
    14881 gcagaacggt tgtcacgtca tgatggaaat tgccaaacga cttcatcagc caggcgatag
    14941 ccgcatttcg cgcggaatgt tcaaattcgg aacgcgctac caccgtatcg taggaaatat
    15001 cagacacacc gcttaagccg cgcacgactt ccagcatacg ttgccgtggt gcgcttaatc
    15061 gcccttgcaa catatcgcag accaccagcg caccagcatt aataaacgga ttacgcggaa
    15121 taccctgctc catttccagt tgcactaaag aattgaacgg tgatccagac ggatctttgc
    15181 cgacgcgttg ccagatttcc tcttcggagt aatgacgcat ggcgacaacg agactcagca
    15241 ctttggaaat agactgaatg gaaaaacgtt cttgcgcgtc tccggcctga aaaagctgtc
    15301 cgtcaacggt acagatagca atgcccaatc gggaaccgtc tactgtagcc agcgccggaa
    15361 tataatccgc gactttaccc tgaccaatga gcggccgcac ttgccgcaag atgttttcta
    15421 aaattgcatt atccatggcg actgccactt tctactcctg gaccgcaggt ctgaaaagac
    15481 ctgcgagtat atcagagctg aatatgtcgc gtcagatccg gtctttccac accgtctgga
    15541 tattacagaa ttcgtgtaag ccgaaatggg aaagctcacg accaaagcca ctctttttca
    15601 cgccaccaaa ggccactcgc gcgtcgctgg cacaataacc attgataaac accccaccgc
    15661 attccagacg tgccgccatc tgtctggcct gtgtttcgtc ggtggtaaaa atggtcgctg
    15721 aaaggccgaa ctcactatca ttagccagtt ccagcgcatg ttctgcatct ttcgcaatgg
    15781 tgattgccgc aacggggcca aacatttctt cccgaaaagc ggtcatttct ggggtaacat
    15841 tcgccagaac cgttggcgga tagtagttac ctgccccagc catcttttcc ccgcccagta
    15901 acaaacgcgc accctgcgcc agggttttct ccacctgatg atgcagctca tcacgtaaat
    15961 cgaaacgagc cattggtccg agagcgttct cttcgtcacg gggatcgtcc attttcaagg
    16021 ctgccgcagc tgccacaaaa cgttcggtaa atgccgaagc aattccctct tcgataataa
    16081 agcgttttgc cgctgcacaa acctgtccgg tattctgata acgtccggct accgccgctt
    16141 taactgccag ttccagatcg gcatcgttaa gcacaataaa tggatccgaa ccgcccagtt
    16201 ccagtacgca ttttttcagt gccgctccag cctgtgcacc aatagccgct ccagcacgaa
    16261 cacttccggt caccgtgata gcagcaatgc gcgagtcttt aatcatctga gtgacaccgt
    16321 cattgtcggc attcagccag ccatatacgc cttgtgggat tcccgcatct ttaaacacct
    16381 gggcaatgag ctgagcacag cccatcacat tcggcgcatg tttaagtaag tatccgttac
    16441 cagcaagaat gatgggaaca gcgccacgca tcacctgcca taacggaaaa ttccacggca
    16501 taatcgccag aatcgtcccc aacggtcgat actcaataac tgcctgctga ttttccacca
    16561 gcgtaggttc cgccttcagc attgccggac catgttctgc ataccagtca cacaaattcg
    16621 ccgatttcgc cacttcagcg cgcgcctggt tgattggttt gcccatttcg cgggtgatca
    16681 tttgcgccat ttcttcgcta cgggcgcgca gagccttacc gatatcacgc agtttttcag
    16741 cacgataatc tatatttgtc tcgcgccagt cacgaaagcc tgctgccgcc agctgaagtg
    16801 cgttttcgat atcatcagcg ccagcccacg gcaacacaga aagttgttca cccgtggcag
    16861 gatttatcga aattgcatga gttgccggag taatggtcat cggggtatct cctttatgag
    16921 tcatggtatg aagatacgca gatttactct tgctttaaaa tgaataatat taagccactt
    16981 attcacgaat cgagaatatt atggatctga cccaactgga gatgttcaac gcggttgccg
    17041 aagctggcag cattacgcag gctgcagcaa tagtgcatcg cgtgccgtcg aatctcacta
    17101 cccgtctacg ccagctggaa acagaactgg gggtagatct gtttattcgc gagaatcagc
    17161 gtttacgtct atctcctgcc gggcataact ttttacgcta tagccaacaa attctcgcgt
    17221 tagtggatga agcgcggagc gttgtcgctg gcgatgagcc gcaaggttta ttttctctgg
    17281 gttcgctgga aagcaccgca gcagttcgca ttccggccac gctggcggaa ttcaaccgtc
    17341 gttatcccaa aattcagttt tcactttcca ccggcccttc cggcactatg ctggaaggtg
    17401 tgctggaggg aaaacttaat gcggcgttta ttgatggacc cattaaccat actgccattg
    17461 acgggatacc ggtataccgc gaggagctga tgatcgtcac gccacaaggt catgcgccag
    17521 taatccgtgc cagtcaggtt aatggcagta acatttatgc cttccgcgcc aattgttcgt
    17581 atcgtcgcca cttcgagagc tggtttcatg ctgacggtgc cgctccggga actatccatg
    17641 agatggagtc ttatcacgga atgttggcct gtgtggtcgc aggagcaggt attgcgctta
    17701 ttccgcgctc tatgctggaa agtatgccgg ggcatcacca ggttgaagcg tggccgttag
    17761 ctgagcaatg gtgttggcta acaacctggc tggtctggcg tcgtggtgcg aaaacgcgtc
    17821 cgctcgaggc atttattcaa ctactggatg tgcctgactc gacaaaacag ggttaccaat
    17881 gagctatttt tgatagttct tgcggctaat atgctctata tagtgatgtt ccgatgactt
    17941 atgactatat ggggcaaata tggttacgcc agtaagcatc agcaattaca tatctcttcc
    18001 tgatgatttt cccgttcgta atattgcacc gcaggtaaaa gaagttttaa aggatttta
//
LOCUS       DAZKIP010000088        18034 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZKIP010000088 DAZKIP010000000
VERSION     DAZKIP010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18034
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.088"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            301..1278
                     /gene="glaH"
                     /locus_tag="ACHOUK_003266"
     CDS             301..1278
                     /gene="glaH"
                     /locus_tag="ACHOUK_003266"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFP9898656.1"
                     /translation="MNALTAVQNNAVDSDQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGV
                     DDVKQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPMARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIEISKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1298..2566
                     /gene="lhgO"
                     /locus_tag="ACHOUK_003267"
     CDS             1298..2566
                     /gene="lhgO"
                     /locus_tag="ACHOUK_003267"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFP9898657.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIEREWLNADELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSGLSEHKSGVVIRTRQGSDYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2589..4037
                     /gene="gabD"
                     /locus_tag="ACHOUK_003268"
     CDS             2589..4037
                     /gene="gabD"
                     /locus_tag="ACHOUK_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFP9898658.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPVWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            4051..5331
                     /gene="gabT"
                     /locus_tag="ACHOUK_003269"
     CDS             4051..5331
                     /gene="gabT"
                     /locus_tag="ACHOUK_003269"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFP9898659.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHNKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            5569..6969
                     /gene="gabP"
                     /locus_tag="ACHOUK_003270"
     CDS             5569..6969
                     /gene="gabP"
                     /locus_tag="ACHOUK_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFP9898660.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYADVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLWMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            6990..7652
                     /gene="csiR"
                     /locus_tag="ACHOUK_003271"
     CDS             6990..7652
                     /gene="csiR"
                     /locus_tag="ACHOUK_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFP9898661.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7653..8102)
                     /gene="kbp"
                     /locus_tag="ACHOUK_003272"
     CDS             complement(7653..8102)
                     /gene="kbp"
                     /locus_tag="ACHOUK_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFP9898662.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8186..8344)
                     /gene="yqaE"
                     /locus_tag="ACHOUK_003273"
     CDS             complement(8186..8344)
                     /gene="yqaE"
                     /locus_tag="ACHOUK_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFP9898663.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8527..8826
                     /gene="ygaV"
                     /locus_tag="ACHOUK_003274"
     CDS             8527..8826
                     /gene="ygaV"
                     /locus_tag="ACHOUK_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFP9898664.1"
                     /translation="MTELTQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            8836..9354
                     /gene="ygaP"
                     /locus_tag="ACHOUK_003275"
     CDS             8836..9354
                     /gene="ygaP"
                     /locus_tag="ACHOUK_003275"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFP9898665.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRRDQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVILGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQ"
     gene            complement(9407..9811)
                     /gene="stpA"
                     /locus_tag="ACHOUK_003276"
     CDS             complement(9407..9811)
                     /gene="stpA"
                     /locus_tag="ACHOUK_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFP9898666.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            10479..10928
                     /gene="alaE"
                     /locus_tag="ACHOUK_003277"
     CDS             10479..10928
                     /gene="alaE"
                     /locus_tag="ACHOUK_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFP9898667.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(10965..11309)
                     /gene="ygaC"
                     /locus_tag="ACHOUK_003278"
     CDS             complement(10965..11309)
                     /gene="ygaC"
                     /locus_tag="ACHOUK_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFP9898668.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            11461..11790
                     /gene="ygaM"
                     /locus_tag="ACHOUK_003279"
     CDS             11461..11790
                     /gene="ygaM"
                     /locus_tag="ACHOUK_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFP9898669.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            complement(11940..13274)
                     /locus_tag="ACHOUK_003280"
     CDS             complement(11940..13274)
                     /locus_tag="ACHOUK_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFP9898670.1"
                     /translation="MPRYQDIARQLKTVIEQGKLKPGARLPSSRTWSQELGVSRSTVE
                     NAYAELVAQGWLVRRGQAGTFVSEQIHPQQSAVDVVAFAGESQQPLPFQMGLPALDLF
                     PRELWARVMGRRLRTQTRFDLALGDVCGEAALREAIVDYLRVSRGIDCQPEQVFITHG
                     YAASMALILHALAQPGDGMWMEDPGFPLIRPIVTRHGVEILPVPVDDNGLDVTSGIQN
                     YPDARFALITPAHQSPLGVALSLARRYQILEWAERSQAWIIEDDYDSEFRYHGKPLPA
                     LKSLDAPQRVIYAGTFSKALFPALRCAWLVVPVEQISQFRQQASLEPCAVPVLWQNTL
                     ADFIREGHFWRHLKKMRQHYAQRRQWIEQALTQQGFQVVPQKGGIQMVIKLAGNDVTF
                     VHKANAAGLAVQALSDWRIRSSEDGGLLLSFTNIVSESMARQVAQQLRSALN"
     gene            13362..13793
                     /locus_tag="ACHOUK_003281"
     CDS             13362..13793
                     /locus_tag="ACHOUK_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFP9898671.1"
                     /translation="MTTLRQPYYELSPAVYNALVQAKTALENSTLDTTLMELIYLRVS
                     QINGCAFCLEMHSKALRKSGVPQHKLDALAGWRVSHHFDERERAALAWAESVTDIART
                     HAEDEVYQPLLEHFSAAEISDLTFAIGLMNCFNRLAVSMRM"
     gene            14002..14247
                     /gene="nrdH"
                     /locus_tag="ACHOUK_003282"
     CDS             14002..14247
                     /gene="nrdH"
                     /locus_tag="ACHOUK_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFP9898672.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            14244..14654
                     /gene="nrdI"
                     /locus_tag="ACHOUK_003283"
     CDS             14244..14654
                     /gene="nrdI"
                     /locus_tag="ACHOUK_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFP9898673.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            14627..16771
                     /gene="nrdE"
                     /locus_tag="ACHOUK_003284"
     CDS             14627..16771
                     /gene="nrdE"
                     /locus_tag="ACHOUK_003284"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFP9898674.1"
                     /translation="MATTTAECLTQKTMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADVAISQHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTGKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            16781..17740
                     /gene="nrdF"
                     /locus_tag="ACHOUK_003285"
     CDS             16781..17740
                     /gene="nrdF"
                     /locus_tag="ACHOUK_003285"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFP9898675.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEYEDWNF"
BASE COUNT         4328 a         4599 c         4684 g         4423 t
ORIGIN      
        1 taatatattt agtttactag cggtcatttt gcagtgaagc catttaccgt tttttatcga
       61 ccagataatc tgttctctga tggtaatccc cccctaacct gttgctttag ttattcattt
      121 cctgtctcac tttgccttaa taccctacgt taaatgttac caatttgttg ctttttatca
      181 caataagaaa acaatatggc gcatttgtgc gcatttttca gaaatgtaga tatttttaga
      241 ttatggctac gaaatgagca tcgccatgtc accctacatc tcataagagg atcgcttctg
      301 atgaatgcac tgaccgccgt acaaaataac gctgtcgatt cagaccagga ctatagcgga
      361 ttcactctca tcccgtcggc gcaatccccg cgtctgctgg aactcacctt caccgaacag
      421 acgaccaaac agtttctcga acaggttgcc gaatggcccg tgcaggcgct ggagtacaaa
      481 tcgtttctgc gttttcgggt aggtaaaatt cttgacgatc tgtgtgcgaa ccagctgcaa
      541 ccattgctgt tgaagaccct gctaaaccgt gctgaaggtg cgctgttgat caatgcggtg
      601 ggtgtcgatg atgtgaagca agcggatgag atggtgaagc tggcgacggc ggtggcgcat
      661 ctgattggcc gttccaattt cgatgcgatg agcggtcagt attacgcgcg ttttgtagtg
      721 aaaaatgtcg ataactcaga cagctatctg cgtcagccgc accgcgtaat ggagctgcac
      781 aacgacggca cctacgtcga agagatcact gactatgtgc tgatgatgaa aatcgacgag
      841 cagaacatgc agggtggaaa ttcgttgctg ctgcatctcg atgactggga acatctggac
      901 cactatttcc gccatccaat ggcgcgtcgc ccgatgcgct ttgccgcgcc gccgagcaaa
      961 aacgtcagca aagatgtttt ccatccagtg ttcgacgttg atcaacaggg tcgcccggtg
     1021 atgcgctata tcgaccagtt cgtccagcca aaagacttcg aagaaggcgt gtggttgagc
     1081 gagctttcgg acgccattga aatcagtaaa ggcattcttt ctgtacccgt tcccgttggc
     1141 aaattcctgt tgattaacaa cctgttctgg ctgcacggtc gcgaccgctt tactccgcac
     1201 ccggatctgc gccgtgaact gatgcgtcag cgtggctatt tcgcttacgc cactcaccac
     1261 tatcagacgc atcagtaagc gcaaaggaat tgagcggatg tatgattttg tgattattgg
     1321 cggcggcatc atcggcatgt cgaccgccat gcaactgatt gatgtctatc cggatgcccg
     1381 cattgcgttg ctggaaaaag agtccggccc ggcctgtcat cagacgggcc acaacagcgg
     1441 cgtgatccat gccggggttt attacacgcc cggtagcctg aaggcgcagt tttgcctggc
     1501 gggaaaccgc gccactaaag ccttttgcga tcaaaacggc attcgctatg acaactgcgg
     1561 caagatgctg gtcgccacct ctgaactcga aatggaacgg atgcgcgcgt tgtgggaacg
     1621 cacggcggca aacggtatcg agcgcgagtg gttaaacgcc gatgaactgc gcgagcgcga
     1681 accgaatatc accgggcttg gcggtatttt tgtgccgtcc agcggcattg tcagctaccg
     1741 cgaagtcacg gcggcgatgg caaaaatttt ccaggccaga ggcggcgaga ttatctataa
     1801 cgccgaagtc agcggcctta gtgagcataa aagcggcgtg gtgatacgta cccgtcaggg
     1861 cagcgactat gaagcatcga cgctgattag ctgttccggg ctgatggctg accggctggt
     1921 gaaaatgctc ggcctcgaac cgggctttat tatctgcccg ttccgcggcg agtatttccg
     1981 cctggcgccg gagcataacc agattgttaa ccatctgatt taccccattc ccgacccggc
     2041 aatgccgttt ttgggcgtgc atctcacccg catgatcgac ggcagcgtca ccgtcgggcc
     2101 aaacgcggtg ctggctttca aacgcgaagg ctatcgcaag cgcgacttct catttagcga
     2161 cacgctggag attttgggct cgtcggggat tcgccgggtg ctgcaaaacc atctacgctc
     2221 aggactgggc gagatgaaaa actcgctgtg caaaagcggc tatctgcggc tggtgcaaaa
     2281 gtattgtccc cggctttcgt taagcgatct ccagccctgg cccgccggtg tgcgggcgca
     2341 ggcggtatcg ccggacggca agctgattga cgattttctg tttgtcacca ccccgcgcac
     2401 gatccacacc tgcaatgcgc cctccccggc agcgacatca gcaattccta ttggtgcgca
     2461 tattgtcagc aaggtacaaa cgctgttggc aagccagagt aaccccggac gcacgctgcg
     2521 agcggcacgt agtgtggatg ccttacacgc cgcgtttaat caataacctt tgaaaacagg
     2581 atgtagcgat gaaactgaac gacagtaact tattccgcca gcaggcgttg attaacgggg
     2641 aatggctgga cgccaacaat ggtgaagtca tcgacgtcac caatccggcg aacggcgaca
     2701 agctgggtag cgtgccgaaa atgggcgctg atgaaactcg cgccgctatc gacgccgcca
     2761 accgcgccct gcccgtctgg cgtgcgctca ccgccaaaga acgcgccaac attctgcgca
     2821 actggttcaa tttgatgatg gagcatcagg acgatttagc gcgcctgatg accctcgaac
     2881 agggtaaacc gctggctgaa gcgaaaggcg aaatcagcta cgccgcctct tttattgagt
     2941 ggtttgccga agaaggcaaa cgcatttatg gcgacaccat tcccggtcat caggccgata
     3001 agcgcctgat tgtgatcaag cagccgattg gcgttacagc ggccatcacg ccgtggaact
     3061 ttccggcggc aatgattacc cgcaaagccg gtccggcgct ggcggcaggc tgcaccatgg
     3121 tgctgaagcc cgccagtcag acgccgttct ctgcgctggc gctggcggag ctggcgatcc
     3181 gcgcgggcat tccggctggc gtgtttaacg tggtcaccgg ttcggcgggc gcggtgggta
     3241 atgaactgac cagcaacccg ctggtacgca aactgtcgtt taccggctcg accgaaattg
     3301 gccgccagtt aatggaacag tgcgcgaaag acatcaaaaa agtgtcgctg gagctgggcg
     3361 gcaacgcgcc gtttatcgtc tttgacgatg ccgatctcga taaagccgtg gaaggcgcac
     3421 tggcctcgaa attccgcaac gccgggcaaa cctgcgtctg cgccaaccgt ttatacgtgc
     3481 aggacggcgt gtatgaccgc tttgccgaaa aattgcagca ggcggtgagc aaactgcata
     3541 tcggcgacgg gctggataaa ggcgtcacca tcgggccgct gatcgatgaa aaagcggtag
     3601 caaaagtgga agagcatatt gccgatgcgc tggaaaaagg cgcgcgcgta gtttgcggcg
     3661 gtaaagcaca tgaacgcggc ggcaacttct tccagcccac cattctggtg gacgtgccgg
     3721 ccaacgccaa agtgtcgaaa gaagagacct tcggccccct cgccccgctg ttccgtttta
     3781 aagatgaagc cgatgtgatc gcgcaagcca atgacaccga gtttggcctt gccgcctatt
     3841 tctatgcccg tgatttaagt cgcgtcttcc gcgtgggcga ggcgctggag tacggcatcg
     3901 tcggcatcaa taccgggatt atttccaatg aagtggcccc gttcggcggc attaaagcct
     3961 cgggtctggg tcgtgaaggt tcgaagtatg gcatcgaaga ttacttagaa atcaaatata
     4021 tgtgcatcgg tctttaactg gagaacgcga atgagcagca ataaagagtt aatgcagcgc
     4081 cgcagtcagg caattcctcg tggcgttggg caaattcacc ccattttcgc tgatcgcgcg
     4141 gaaaactgcc gggtgtggga cgttgaaggc cgtgagtatc ttgatttcgc gggcggcatt
     4201 gcggtgctca ataccgggca cctgcatccg aaagtggttg ccgcggtgga agcgcagttg
     4261 aaaaaactgt cgcacacctg cttccaggtg ctggcttacg agccgtatct ggagctgtgc
     4321 gagattatga atcagaaggt gccgggcgat tttgccaaga aaacgctgct ggttacgacc
     4381 ggttccgaag cggtggaaaa cgcggtgaaa atcgcccgcg ctgccaccaa acgtagcggc
     4441 accatcgctt ttagcggcgc gtatcacggg cgcacgcatt acacgctggc gctgaccggc
     4501 aaggtgaatc cgtactctgc gggcatgggc ctgatgccag ggcacgttta tcgcgcgctt
     4561 tatccttgcc cactgcacgg catcagtgaa gatgatgcta tcgccagtat ccaccgaatt
     4621 tttaaaaatg atgctgcgcc ggaagatatc gccgccatcg tgattgagcc ggttcagggc
     4681 gaaggcggtt tctacgccgc gacgcctgcg tttatgcagc gtttacgcgc gctgtgtgac
     4741 gagcacggga tcatgctgat tgccgatgaa gtgcagagcg gcgcggggcg taccggcacg
     4801 ctgtttgcga tggagcaaat gggcgtggca ccagatctca ccacctttgc gaaatcgatc
     4861 gcaggcggct tcccactggc gggcgtcacc gggcgcgccg aagtgatgga tgccgtcgct
     4921 ccaggcgggc tgggtggcac ctatgccggt aatccgattg cctgcgtggc ggcgctggaa
     4981 gtgttgaagg tgttcgagca ggaaaatctg ctgcagaaag ccaacgatct ggggcagaag
     5041 ttgaaagatg gattgttggc gatcgccgaa aaacaccctg agatcggcga cgtacgcggg
     5101 ctgggggcga tgatcgccat cgagctgttt gaagacggcg atcacaacaa gccggacgcc
     5161 aaactcaccg ccgagatcgt ggctcgcgcc cgcgataaag gcctgattct tctctcctgc
     5221 ggcccgtatt acaacgtgct gcgcatcctt gtaccgctca ccattgaaga cgctcagatc
     5281 cgtcagggtc tggagatcat cagccagtgt tttgctgagg caaagcagta gcgccgctcc
     5341 tatgccggag gcgacgctgc gcgtcttgtc cggcctacgg ggatccaggt cggataaggc
     5401 gtttacgccg catccgacaa tctgtacgtg aacaggaata aatctatgtt ggccgggtaa
     5461 ggcggagccg ctctccggca aaaagaatca ataacaatta tacgcgtgac ccgacgcggg
     5521 aaatgtcggg gcgctctccc aagtgacaca ctttcgagag gattcaggat ggggcaatca
     5581 tcgcaaccac atgagttagg cggcgggctg aagtcacgcc atgtcaccat gttgtctatt
     5641 gccggtgtta tcggcgcaag tctgtttgtc ggttccagcg tcgccatcgc cgaagcgggc
     5701 ccggcggtat tactggccta tctgttcgcc gggctactgg tggttatgat tatgcggatg
     5761 ttggcggaaa tggcggttgc cacgcccgat accggttcgt tttccaccta tgccgataaa
     5821 gctattggtc gttgggcggg ttataccatc ggctggttgt actggtggtt ttgggtactg
     5881 gttatcccgc tggaagccaa catcgccgcc atgatcctgc actcatgggt tccaggcatt
     5941 cccatctggt tattttccct cgtcattacc cttgccttaa ctggcagtaa tttattaagc
     6001 gttaaaaact acggcgaatt tgagttctgg ctggcgctgt gcaaagtcat cgctatcctg
     6061 gcctttattt tccttggtgc agtcgcaatt agcggttttt acccgtatgc cgacgtgagc
     6121 gggatctcaa gattgtggga tagcggcggc tttatgccca acggcttcgg tgcggtatta
     6181 agcgcgatgt tgatcaccat gttctcgttt atgggcgcag aaattgtcac cattgccgcc
     6241 gcggaatccg acacgccgga aaaacatatt gtccgcgcta ccaactcggt tatctggcgt
     6301 atttctatct tctatttgtg ctctattttt gtcgtagtgg cgttaatacc gtggaatatg
     6361 ccggggctga aagccgttgg atcttatcgc tcggttctgg aattgctcaa tataccccat
     6421 gcgaaattaa tcatggactg cgtgatatta ctttccgtaa ccagttgcct gaactcggcg
     6481 ctgtataccg cgtcaaggat gctctactcc ttaagccgtc gcggtgatgc gcccgcggta
     6541 atgggcaaaa tcaaccgcag taaaaccccg tatgtggcgg tgttactctc caccggagcg
     6601 gcatttttaa cggtcgtggt gaactattac gcacctgcga aagtgtttaa attcctgatc
     6661 gacagctccg gtgctatcgc cctgctggtt tatttagtca tcgccgtttc acagttgtgg
     6721 atgcgtaaaa ttctgcgagc agaaggaagc gaaattcgct tgcgcatgtg gctttacccg
     6781 tggctcacct ggctggtaat cggctttatt acctttgtgt tggtggtgat gttattccgc
     6841 ccggcgcaac agttagaagt gatctctacc ggcttattag cgatagggat tatctgtacc
     6901 gtgccgatta tggcgcgctg gaaaaagctg gtattgtggc aaaaaacacc cgttcataat
     6961 acgcgctgat aatgatcagg agtcacacca tgaccattac gtctctggat ggctatcgct
     7021 ggctgaagaa cgatattatt cgcggtaatt ttcaaccgga tgaaaaatta cgaatgagtt
     7081 tgctgacatc gcgttatgca cttggcgttg ggccgttacg ggaagctctt tcgcaactgg
     7141 tggcggaacg gctggtcacg gtggtgaatc aaaaagggta tcgggtggcg tctatgtcag
     7201 agcaggagct gctcgatatt ttcgacgccc gcgccaatat ggaagcgatg ttagtgagtc
     7261 tggcgattgc ccgcggtggc gatgagtggg aggcagacgt tctcgcaaaa gcgcatctgc
     7321 tgagtaagct tgaggcctgt gacgccagcg agaaaatgct tgatgagtgg gatctgcgtc
     7381 atcaggcgtt tcatactgca attgtggcgg gctgtggttc tcactatttg ctgcaaatgc
     7441 gtgaacggtt gtttgatctg gcggcgcgtt atcgatttat ctggctgcgg cgaacggtgc
     7501 tttcggtgga aatgctggag gataaacacg atcagcacca gaccctgact gcggcggtac
     7561 tggcgcgaga taccgcgcgc gccagtgagt taatgcgcca gcatttactg acgccaattc
     7621 ccattatcca gcaggcgatg gctggcaatt aactactctt ccggaatacg caacacttgc
     7681 cccggataaa ttttatccgg gctttttagc atcggtttat tcgcttcgaa gattttattg
     7741 tacagattag cgttaccgta gacctgtttg gaaatggcac tcagagtgtc gccagactta
     7801 acggtataaa actggctggc agtggctggt gtcgccgttt tcacctgatc atcaacactg
     7861 gcaataccgg aaatattccc caccgcaaca aggattttct ccttcgcctc ctgactcagg
     7921 ccgtcaccag tgaccgtcgc tttgccatcg gcaatttgaa tgttcacttt atcggcatca
     7981 ggtataccgg ttttgctcag atgctcctgc accttcttcg cctgatcatc tttatcgtgc
     8041 tgacctgtaa ccgcgtccca gagtttttct ccggcatctt tcacaaaatt gaacagaccc
     8101 atagctacct cattcgttaa cggaaacact cagaaagtgt agtagaggaa cgcggcgcgt
     8161 taacgccgcg gggatgagca caggtttaat cgcgggtttg cacccagaac gcgtgaatca
     8221 aaccggggat atacccaagc agtgtcaaca gaatattaat aatgaacgcc caaccgaacc
     8281 ctttaccgag cagcacgccg agcggcggca gaatgatggt gatgacgatt ctccagaaac
     8341 ccatatgtac tccctataag aaaattactt attgtttaaa aagagatttt atctcttaag
     8401 cgtagtaatt ttagcggagg ctgccagttt cagcttacgc ttttactctc atttacgctg
     8461 ctgaggctgg tatatgttgc atttttagtt tagagtttac ttaatttaga aaatacttaa
     8521 ataactatga ccgaactcac gcaattacag gccagtgctg aacaggcagc ggccttattg
     8581 aaagcaatga gccaccctaa acggttgctg attctgtgca tgcttagcgg ttcccccggc
     8641 accagcgcgg gagagctgac gcgcattacc ggactgagcg cctctgcgac atcacagcat
     8701 ctcgctcgta tgcgggacga agggcttatc aacagccaac gggatgccca acgcattcta
     8761 tattccatta aaaatgaggc ggtaaatgcc attatcgcca ccctgaaaaa tgtctattgt
     8821 ccgtaaggag tcactatggc tttgacaacc atttcgccgc atgatgcaca agaattaatc
     8881 gcacgcggcg caaagttaat cgatattcgt gacgctgatg aatatcttcg tgaacatatt
     8941 cctgaagcag atctggctcc attatccgtg ctggaacagt caggtctccc ggctaaatta
     9001 cgtcgcgatc aaattatatt ccactgccag gcaggtaaac gcaccagtaa taacgccgat
     9061 aaattagcag cgattgccgc ccccgcagaa atctttttac tcgaagatgg gattgatggc
     9121 tggaaaaaag caggattacc agtagcggta aataaatctc aacccttgcc gttaatgcgc
     9181 caggtgcaga tcgctgcggg tggtttaata ttaatcggcg ttatactggg ttataccgta
     9241 aatagcggtt tcttcttatt aagcggcttt gttggtgccg ggttactgtt tgcaggaatt
     9301 agcggttttt gtgggatggc aaggttgtta gataagatgc cgtggaacca atgagcttga
     9361 gaagcgacgc cggacgcgcc ctagcagcga catccggcct cagtaattag atcaggaaat
     9421 cgtcgagaga tttaccttct gccagcgcct gagcgattgg cttcggtgta cggccctgac
     9481 cggtccaggt tttagtttca ccgttaacat cggtgaattt atatttcgcc ggacgcggct
     9541 ggcgtttttt accagcacgt ggtgcagcag cagagctatt acccaataac tcttccgggt
     9601 taattccgtc agctttcatc agctccagcc aggtgctaat tttttcctgg cgctctgcca
     9661 gttcacgctg ctgctgttct tcttcttcac gtctttcttt agtgacaacc ctgaattttt
     9721 cgagcatttc ttcaagaacg tcaatggaga attcgcgagc catcgcacgg agggtgcgaa
     9781 tgttatttaa actttgtaac attacggaca taataaagaa aaccttttaa cgccaaaaca
     9841 aaaaagtatt tcgtcgcgta gtgtaagcga tcacttatta tatttccaca aaatttaatc
     9901 tatttacagt cctgcgcgag attatttcag ctgttatttt ctgaaactct cagaataatt
     9961 tcaagattat atattagcca ttttgatgag taatttagat tattggtgaa cagaacggcg
    10021 ctctctgctc gctaattccg caagcctgac aaaaccaaag tgaataaaaa tgcaatataa
    10081 cagaataaaa taaaacaccc ctcactggtg ctttctgctg ttaatgtaca ttttctggca
    10141 taaaaaatgc tgtaaatgac ccggtcacga catacaccga attctctacg cttatagcgt
    10201 tacctcaccc ccaaactttc attagtgaaa ttaaaggaaa ggtcatatcc aattgattta
    10261 attgaaaata aatcatatcg gcatgtaaaa atccacaatg cacaaaaaac gagccgttac
    10321 ggaatatttt atctacaaaa actgactaaa taaaaatttt tcactaattg attagtcata
    10381 gccagcgata tacgctatgc gaaaatgcag atggcaatga gatccactgc tttcatctcc
    10441 attaacatcc cattacgctt ttattaagga gcattagcat gttctcaccg cagtcacgct
    10501 tgcgtcatgc agttgcagat acgttcgcga tggttgttta ctgttctgtc gtgaacatgt
    10561 gtattgaagt tttcctctcc ggaatgagct tcgaacagtc tttttattcc agattggtag
    10621 cgattccggt gaacatctta attgcatggc catacggtat gtaccgcgat ctgtttatgc
    10681 gcgcggcacg caaagttagc ccgtcgggct ggataaaaaa tctggcggat atcctggctt
    10741 atgtgacgtt ccagtcaccg gtgtatgtgg cgatcttgtt agtggtgggc gcagactggc
    10801 atcagattat ggcggcggtc agttcaaaca tcgttgtttc gatgttgatg ggggcggttt
    10861 atggctactt cctcgattat tgccgccgac tgtttaaagt cagccgttac cagcaggtaa
    10921 aagcctgact cttcttttcg cgactggcat cgccagtcgc gtctttaact ggcttcgcca
    10981 aataaaccat tcaaataacg ttcaagcgca acacgcgaac taaagccatg cgggataccg
    11041 taatgctcgt gtcgctcgcc tgctaaatag agcggaaatt gctggtaatg atcgcaagtt
    11101 ttaatatcgg aagcgggttc agccagtgtc gaactgcgtt cttttaatgt tggatgaatt
    11161 accagagcgg tacgtcccat tcgcgcttcg cgattaacat agacataatt ttccccacgg
    11221 cggtaaccat acgctttctg ggttaccaca tcgacagtaa aaccgacttt ttcaagtacg
    11281 cgcgccacct cgtctggtcg taaatacata tttgatcctc gttattatca accgcgggcc
    11341 taccttacct gattgcgcat tcacaacgct ttcagaaaag tccataaagc cgcgatgaac
    11401 ctcagttaag aaaattatgg tctacactga aaattacatc gaattctaat gggggatcat
    11461 atgtttaacc gaccgaaccg caacgacgtt gatgatggcg tgcaggatat ccagaatgat
    11521 gtcaatcaat tagctgacag cctcgaatct gtattgaagt cctggggcag cgacgccaaa
    11581 ggggaagctg aagccgcacg cagtaaagct caggcattgc tgaaagaaac cagggcgcga
    11641 atgcatggtc gtactcgcgt ccagcaagcc gcgcgcgatg ccgtaggctg cgcggattct
    11701 tttgttcgtg aaagaccctg gtgtagtgtg ggaactgctg ctgcggtagg tatttttatc
    11761 ggtgcgttgt tgagcatgcg taaatcgtag tgagacgatg acaagcagta gtctgacttc
    11821 tgaaaagtac tcagtgcgtc aacgccttgt ccgcctacag gcctgaaaaa catagcgcat
    11881 caggggattt tgccctggtc accagtctta taggaccccg aagcctggct tcggggttat
    11941 taatttaaag cactgcgcaa ttgctgtgct acctgtcgcg ccatactttc gctaacgata
    12001 ttcgtaaacg agagcaataa tcctccgtcc tcgcttgagc ggatacgcca gtcgctaagt
    12061 gcctgtacgg caagaccagc agcattggct ttatgcacga atgtcacgtc attacctgct
    12121 agtttgatca ccatctggat accgcctttt tgcggcacaa cctgaaatcc ctgctgcgta
    12181 agtgcctgct caatccactg ccgtcgctgg gcataatgct ggcgcatttt cttcagatgc
    12241 cgccagaaat gcccctcacg gatgaaatct gccagtgtgt tctgccacag tacgggtaca
    12301 gcacatggtt ccagtgacgc ctgctgccgg aattgcgaaa tctgctccac cggcaccacc
    12361 agccacgcac agcgcaatgc aggaaatagc gctttgctga atgttccggc ataaatcacc
    12421 cgctgcggcg cgtcgagact ttttagtgcc ggtaacggct taccgtgata gcgaaactca
    12481 ctgtcgtaat catcttcaat aatccatgcc tgactacgct ctgcccattc cagtatctga
    12541 tacctacgcg ctaaagagag tgccacaccc agcgggcttt ggtgtgccgg agtaatcagg
    12601 gcaaaacgcg catcaggata attttgtatt ccgcttgtga catccagtcc gttgtcatca
    12661 accggcacag gtaaaatttc cacaccgtgg cgagtgacaa tcgggcgaat cagcggaaag
    12721 ccgggatctt ccatccacat cccgtctccc ggttgcgcca gagcgtgcag aattaaagcc
    12781 attgaggccg cataaccgtg agtgataaag acctgctctg gctgacaatc aatcccacgt
    12841 gaaacgcgta aataatcaac aatagcctcg cgcaaggccg cctcgccgca gacatcgcct
    12901 aatgccaaat caaagcgcgt ctgggtacgc agacggcggc ccatcacccg cgcccacaat
    12961 tcacgcggaa aaagatcaag tgcaggtagc cccatctgaa aaggcagcgg ctgctgactt
    13021 tcaccagcaa aagccacgac atccacagca gactgttgcg gatgtatctg ctcactgaca
    13081 aaggtacccg cctgtccccg cctgaccagc catccctgcg ccaccagctc agcataagca
    13141 ttttcgacag tggatcgaga gacacccagc tcctgcgacc aggtacggct cgaaggtagc
    13201 cttgcgccag gtttcagttt tccttgctcg ataaccgttt ttaactgacg ggcgatatcc
    13261 tgataacgtg gcatatggtc tgctttttta ggtaatttat ggctctcttt agcagtccat
    13321 tatagtgcta cattgcgggc gttaataaga ggagaaactg tatgactaca ttacgtcagc
    13381 cttactacga acttagcccg gcagtgtata acgcactggt gcaggccaaa acggcactgg
    13441 aaaatagcac gctggatacc acgttgatgg agttaattta tttgcgcgtc tcgcaaatca
    13501 acggctgcgc attttgtctg gagatgcaca gcaaagcatt gcgcaaatcc ggtgtgccac
    13561 aacacaaact ggacgccctg gcaggctggc gcgtaagcca tcattttgat gaacgcgagc
    13621 gagcggcgct ggcatgggcg gaatctgtga ccgatattgc cagaacccat gcggaagacg
    13681 aggtttatca gcctttgctt gagcatttca gcgcagcaga aatcagcgac ttaacgtttg
    13741 ccatcgggct gatgaattgt tttaaccgtc tggccgtttc catgcggatg taatttgctg
    13801 ttgccgggta tgcgcccggc aaaaaaacac aaaatcccct accccgttga gccattcacc
    13861 ggtggcgtaa tcaatctaaa attaccatat cttgtgtggt tgaatctttt ttcaactaca
    13921 tctagtatct ctgtatcaac agagagacaa cccgacgcgt atcatcgcgc cgtatcttca
    13981 ttttaaacgg aaatacgaat catgcgcatt actatttaca ctcgtaacga ttgcgttcag
    14041 tgccacgcca ccaaacgggc gatggaaaac cggggctttg attttgaaat gattaatgtc
    14101 gatcgcgttc ctgaagcggc agaagcgttg cgtgctcagg gctttcgtca gttgccggta
    14161 gtgattgctg gcgatcttag ctggtctggt ttccgtccgg acatgattaa ccgtctgcat
    14221 ccagcgccac acgcggccag tgcatgagcc agctcgtcta cttctccagc agctccgaaa
    14281 acacgcagcg ttttatcgaa cgtttaggtc tgcccgcggt gcgcatcccg ctcaatgagc
    14341 gggaacggat tcaggtagac gagccttaca tcctgatcgt gccctcttac ggcggcggcg
    14401 gtacggctgg cgcggtgcca cgacaggtaa ttcgcttttt aaacgacgag cacaaccggg
    14461 cgttgcttcg cggcgttatt gcttcgggta atcgcaactt tggtgaggcg tatggccgcg
    14521 ccggagatgt gattgcccgg aaatgcggcg tgccgtggct gtaccgtttt gaactcatgg
    14581 gtacgcaaag cgatatcgaa aacgttcgta aaggagtaac cgaattttgg caacgacaac
    14641 cgcagaatgc ctgacgcaga aaacgatgga ttaccacgcg ctgaatgcga tgcttaacct
    14701 ctacgatagc gcaggtcgca ttcagttcga taaagaccgc caggccgttg acgcctttat
    14761 ggcgacgcat gtgcgtccga acagtgtgac cttcagtagc cagcagcagc gcctgaactg
    14821 gctggtcaac gaaggttact atgatgaaag cgttcttaat cgctactctc gcgactttgt
    14881 cattacgctg tttgcccacg cacacaccag cggttttcgt ttccagacat tcctcggggc
    14941 atggaagttt tacaccagct atacgttgaa gacattcgac ggtaaacgtt atctggaaga
    15001 ttttgccgat cgagtcacga tggtggcgct gacgctggca caaggcgatg agacgctggc
    15061 gttgcaactg acagatgaaa tgctgtcagg acgctttcag ccagccacgc caacattcct
    15121 caactgcggt aagcagcagc gcggcgaact ggtttcctgt tttttgctgc gtattgaaga
    15181 caatatggag tcgattggtc gggcggtaaa ttccgcactg caactgtcga aacgcggcgg
    15241 cggcgtagca tttttgctgt cgaatctgcg ggaagcgggc gcgccaatta aacgtattga
    15301 aaatcaatct tctggcgtaa ttccggtgat gaaaatgctg gaagacgcat tttcctatgc
    15361 caaccaactc ggcgctcgtc agggggctgg tgcagtctat ttacatgctc atcatcccga
    15421 tattctgcgt tttctcgaca cgaaacggga aaatgccgac gaaaaaatcc gcattaaaac
    15481 actgtcgctt ggcgtggtga tcccggatat cactttccat ctggcaaaag agaatgcgca
    15541 gatggcgctg ttttcgcctt atgacgtaga gcgagtttat ggcaagccgt ttgccgatgt
    15601 ggccatcagc caacactatg acgaactggt tgccgatgaa cgcattcgca aaaaatacct
    15661 caacgcccgt gatttcttcc agcgactggc agaaatccag tttgagtccg gctatcccta
    15721 catcatgtat gaagacacgg taaaccgtgc taaccctgtc gccgggcgca taaatatgag
    15781 taatctctgc tcagaaattt tgcaggttaa cagcgcctca gagtatgacg agaatctcga
    15841 ctatgcccgc acaggccatg atatctcctg caatttaggt tcgttgaata ttgcgcacac
    15901 catggattcc cccgattttg cccgcacggt agagactgcc gtgcgcggtt taacggcagt
    15961 atcagatatg agtcatatcc gcagcgtgcc gtccatcgaa gccggaaatg ccgcctcgca
    16021 cgccatcgga ctggggcaga tgaatttaca cggctatctg gcgcgagaag gcatcgctta
    16081 tggttcgccg gaagcactgg atttcaccaa tctctatttc tatgccatca cctggcacgc
    16141 actgcgtacc tcgatgttgc tggcacgcga acgcggtgaa accttcgccg ggttcaaaca
    16201 gtcacgctat gccagtggtg aatattttag ccaatatctg caagggaact ggcagccgaa
    16261 aacggggaaa gttggcgaac tgtttgcccg tagcggtatt acgttaccta cccgtgagat
    16321 gtgggcgcaa ctgcgcgacg acgtgatgcg ctacggcatt tacaaccaga atcttcaggc
    16381 ggtgccgcca acaggttcta tctcttatat caaccatgct acgtcgagta ttcatccgat
    16441 tgtggcgaaa gtagagatac gcaaagaggg caaaacagga cgcgtttact accctgcccc
    16501 gtttatgact aacgagaatc tggcgctgta tcaggacgct tacgaaattg gcgcagaaaa
    16561 gatcatcgac acctacgcgg aagcgactcg ccatgtcgat caggggctgt cgctgacgct
    16621 gtttttcccc gataccgcca ccactcgcga tatcaacaaa gcgcagattt acgcctggcg
    16681 caaaggtatc aaaacgctct attacatccg gctgcgtcag atggcgctgg aaggcactga
    16741 aattgaaggc tgcgtctcct gtgcacttta aggaatatct atgaaactct cacgtatcag
    16801 cgccatcaac tggaacaaga tatctgacga taaagatctg gaggtgtgga atcgcctgac
    16861 cagcaatttc tggctaccag aaaaggtgcc gctgtcgaac gatattcctg cctggcagac
    16921 attaactgtc gtagaacaac aactgacgat gcgcgttttt actggcctga cgctgctcga
    16981 cacgctgcaa aatgttatcg gcgcgccttc gctgatgccc gatgcactca cgcctcatga
    17041 agaagcggta ttatcgaata tcagctttat ggaagcggtt catgcccgct cttacagttc
    17101 gattttctcg acgctgtgcc agaccaaaga tgtcgatgcc gcctacgcct ggagtgaaga
    17161 aaacgcgccg ttgcagcgaa aagcacagat tattcagcaa cattaccgcg gtgatgatcc
    17221 gctgaaaaag aaaatcgcca gtgtgtttct tgaatctttt ttgttctatt ccggtttctg
    17281 gctgccgatg tatttttcca gccgcggaaa gctgaccaat accgcggacc tgatccgtct
    17341 gattatccgc gatgaagcag tccacggtta ctacataggc tataaatatc agaaaaacat
    17401 ggaaaagata tctctgggac aacgtgaaga gttgaagagt ttcgccttcg atttgttgct
    17461 ggaactctac gacaacgagt tgcaatacac cgatgagctg tacgccgaaa ccccgtgggc
    17521 tgacgatgtg aaagcgtttc tctgttacaa cgccaataag gctttgatga atctgggcta
    17581 cgaaccgtta tttcccgcag aaatggcgga agtgaatccg gcaatcctcg ccgcgctttc
    17641 gccgaatgcc gatgaaaatc acgatttctt ttccggttca ggctcctctt atgtgatggg
    17701 gaaagcggtt gaaacagaat atgaagactg gaatttctga gggtgttatt ttcaaaaata
    17761 tcactacccg cgacagggaa ataattcccg ccaaatagct ttttatcacg caaatatttt
    17821 gtggtgatct acactgatac tctgttgcat tattcgcctg aaaccacaat attcaggcgt
    17881 tttttcgcta tctttgacaa aaaatatcaa cttttctcga tttgctctca gcccttatat
    17941 cacgggaaat tccggcgatt tgctcgcatc aatattcatg ccacatttgc catcaggggt
    18001 tgcctcagat tctcagtatg ttagggtaga aaaa
//
LOCUS       DAZKIP010000089        17937 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZKIP010000089 DAZKIP010000000
VERSION     DAZKIP010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17937
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.089"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            239..1582
                     /locus_tag="ACHOUK_003286"
     CDS             239..1582
                     /locus_tag="ACHOUK_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9898676.1"
                     /translation="MRPISNLNTSAATYIPPQQLPSSCLETLTLLVQMNNYIKRDADY
                     STGMAVAPLVPEEVHLLAAAMTTELHRHQFQPVVSANDLQLPEPFTFDVAGFTITFTK
                     TQEHDNTGNLMKIAVSKSGVCTSTNITLELFHSIVTTLMSRSQYGTFDLWSVRPILTD
                     ESQNRVHEAARYSPAQQYGREETHFTNNGMREFGNMSPLAWRNDVELQQSCNTSNIPP
                     NAANDDINNTRQTIEDQPEADEPQQYVKLTVDDMRKWAAMDQQARNALQGVSGWCTRN
                     HFNIKNARNYLTDHGLNYAGQVKVNRPHEYAKFTLEHIRQWAALNKHVRKPVGYLEKW
                     CKERNLAPTTARNYLKNDGLTALGELKLTGPQKWVTFTFGDIVQWANMTQEERNSAGG
                     AEKWSKKRGFQWSTARSYLKSSGVTKQGARKLAWLKNSGNMSNPFYLAQPTSRRT"
     gene            complement(1731..3239)
                     /gene="putP"
                     /locus_tag="ACHOUK_003287"
     CDS             complement(1731..3239)
                     /gene="putP"
                     /locus_tag="ACHOUK_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFP9898677.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPSVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEG"
     gene            complement(3398..3607)
                     /locus_tag="ACHOUK_003288"
     CDS             complement(3398..3607)
                     /locus_tag="ACHOUK_003288"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9898678.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            3662..7624
                     /gene="putA"
                     /locus_tag="ACHOUK_003289"
     CDS             3662..7624
                     /gene="putA"
                     /locus_tag="ACHOUK_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFP9898679.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGYVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGRGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSTVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(7664..8302)
                     /gene="rutR"
                     /locus_tag="ACHOUK_003290"
     CDS             complement(7664..8302)
                     /gene="rutR"
                     /locus_tag="ACHOUK_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFP9898680.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKSLIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            8590..9681
                     /gene="rutA"
                     /locus_tag="ACHOUK_003291"
     CDS             8590..9681
                     /gene="rutA"
                     /locus_tag="ACHOUK_003291"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFP9898681.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPVLTQEVA"
     gene            9681..10373
                     /gene="rutB"
                     /locus_tag="ACHOUK_003292"
     CDS             9681..10373
                     /gene="rutB"
                     /locus_tag="ACHOUK_003292"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFP9898682.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTS
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFVQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            10385..10771
                     /gene="rutC"
                     /locus_tag="ACHOUK_003293"
     CDS             10385..10771
                     /gene="rutC"
                     /locus_tag="ACHOUK_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFP9898683.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINETYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            10779..11579
                     /gene="rutD"
                     /locus_tag="ACHOUK_003294"
     CDS             10779..11579
                     /gene="rutD"
                     /locus_tag="ACHOUK_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFP9898684.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSITQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDHP
                     ASVTVLVCVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            11589..12179
                     /gene="rutE"
                     /locus_tag="ACHOUK_003295"
     CDS             11589..12179
                     /gene="rutE"
                     /locus_tag="ACHOUK_003295"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFP9898685.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIIACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            12190..12684
                     /gene="rutF"
                     /locus_tag="ACHOUK_003296"
     CDS             12190..12684
                     /gene="rutF"
                     /locus_tag="ACHOUK_003296"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFP9898686.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            12705..14033
                     /gene="rutG"
                     /locus_tag="ACHOUK_003297"
     CDS             12705..14033
                     /gene="rutG"
                     /locus_tag="ACHOUK_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFP9898687.1"
                     /translation="MAMFGLPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASGFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGSSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(14116..14223)
                     /locus_tag="ACHOUK_003298"
     CDS             complement(14116..14223)
                     /locus_tag="ACHOUK_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HFP9898688.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            14595..15191
                     /gene="wrbA"
                     /locus_tag="ACHOUK_003299"
     CDS             14595..15191
                     /gene="wrbA"
                     /locus_tag="ACHOUK_003299"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFP9898689.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMS
                     PQLFEKAGGKTQTAPVATPQELANYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            15212..15439
                     /gene="yccJ"
                     /locus_tag="ACHOUK_003300"
     CDS             15212..15439
                     /gene="yccJ"
                     /locus_tag="ACHOUK_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFP9898690.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(15477..16718)
                     /gene="agp"
                     /locus_tag="ACHOUK_003301"
     CDS             complement(15477..16718)
                     /gene="agp"
                     /locus_tag="ACHOUK_003301"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFP9898691.1"
                     /translation="MNKTLIAAAVAGVVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWRDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDADGFCPMDKFDSVLNEAVK"
     gene            complement(17009..17937)
                     /locus_tag="ACHOUK_003302"
                     /pseudo
     CDS             complement(17009..17937)
                     /locus_tag="ACHOUK_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526794.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4333 a         4741 c         4638 g         4225 t
ORIGIN      
        1 aaaataataa tcttcatgaa actcaccccg atggcagcat tgtaaattcg ccaataactg
       61 gcattcctcc tgctttttac gggtgtttcc attaacacca gaatgagcgg attgtttact
      121 acatcaagaa cgcatccgaa gtgtacatcc tggtgcgccg actgaatcgc ccggagccat
      181 gcggatggct acgacttaat acgaatcatt aaccctgtaa ggatacggag agcattacat
      241 gagaccaata tccaacctca atacgagcgc agcaacctat attccaccgc aacaactccc
      301 gagtagttgt ctggaaactt tgacgcttct ggtgcagatg aataattata tcaaaaggga
      361 tgcggactac agcacgggca tggctgtagc tccactggtt cctgaagagg ttcacttgct
      421 ggctgctgcg atgacaaccg aactgcatcg acatcagttc caaccggtgg taagtgcaaa
      481 tgatctgcaa cttcctgaac cgttcacctt cgatgttgcc ggtttcacca tcaccttcac
      541 gaagacgcaa gagcatgaca acacgggcaa tctcatgaaa attgcagtca gcaagagcgg
      601 tgtgtgcacc agtacaaata ttactcttga gctgtttcac tccattgtca caaccctgat
      661 gtcacgcagt caatatggaa cctttgatct ctggtccgta cggccaatac tcacggatga
      721 aagtcaaaac agggtgcatg aagcagctcg ctactccccg gcccagcaat acggaagaga
      781 agaaacacac tttaccaaca atggcatgcg agaatttgga aatatgtctc cgctggcgtg
      841 gcgtaacgat gtggaattac agcaatcgtg taatacaagc aacatcccac caaatgctgc
      901 aaatgacgac ataaacaaca ccaggcagac catcgaggac cagccagagg cggacgagcc
      961 acagcaatat gttaaattga ctgtagacga catgcgaaaa tgggcggcta tggaccagca
     1021 ggcgcgaaac gctctacagg gagtttcagg atggtgtacg cgcaatcatt ttaatatcaa
     1081 aaatgccaga aattacctga ccgatcacgg cctgaactac gcgggacagg taaaggtaaa
     1141 caggccgcat gaatatgcca aattcaccct tgaacatatc cgccagtggg cagccttaaa
     1201 caagcatgtg cgaaaaccgg ttggttattt agaaaagtgg tgtaaggaaa gaaacctcgc
     1261 gccaactact gccaggaatt acctgaagaa cgacgggctt accgccctgg gtgagttgaa
     1321 gctgacaggg ccgcagaaat gggtcacgtt caccttcgga gacatcgtac aatgggcgaa
     1381 tatgacccag gaggagcgta acagcgcagg gggagccgaa aagtggagta aaaaacgcgg
     1441 cttccagtgg tctactgcca gatcctacct gaaatcgagc ggagtgacca agcaaggtgc
     1501 gagaaaactg gcgtggttaa aaaacagcgg gaatatgtcg aatccattct atctcgccca
     1561 accaaccagc cgcaggacat aagctgaagg taccgatagc ggcaattttg ttcatctgtg
     1621 ccgggtgggc aaatagcaat tgcccccgaa cactccctgc caggcaactt ataccgcatg
     1681 gcagggaaca ttcgggttaa aaagcaaaag ataactaatt actcttcagc ttagccttcc
     1741 tgcaaccgtg acggcggagc cgaatgatag tgcgcatcgg cctcggcaaa gcgtttttgc
     1801 atcgccgctg acggtgcttt acccagcaaa ctaaacacta caatcccaat actgccaaag
     1861 ataaagcccg gaataatttc gtacagtccc agccagccga actgtttcca gacgatcacc
     1921 gtcagcgcac cgatgatcat ccccgccagc gcaccgttgc gcgtcatgcg tgaccacatc
     1981 accgaaaaca gcaccaccgg accaaacgcc gcgccaaagc ctgcccacgc gtagctcact
     2041 aagcccagca cgcggttttc cgggttcgct gccaacgcaa tcgccaccag cgccaccacc
     2101 agcaccatca cacgccctac ccacaccagt tctttctggc tggcatgttt acgcagaaac
     2161 gctttgtaca aatcttcggt aatcgcactg gaacatacca gcagctggca acttaacgtt
     2221 gacattaccg ccgccagaat cgccgacagc agaatcccgg caatccacgg gttaaacaga
     2281 atttgcgcca gttcgataaa cacgcgctcg gcgttctggt ttaccgcacc agctaccgac
     2341 gggtgctcgt taaagtaagc gataccaaag aagccgacag ccaccgcccc tgccaggcag
     2401 aggatcatcc aggtcatact gatacgacgc gcatggacga tgctgtggtg agaatccgcc
     2461 gccataaaac gcgccaggat gtgcggttgc ccgaagtaac ccagccccca ccccatcagc
     2521 gagataatgg cgacaaagtt cagccctttg agcatatcca cgttttcgat gctcttttgt
     2581 ttgatcactt ccagcgagtc accaaagcca ccgacactga taatgacgat aaccggcgtc
     2641 aggatcaggg caaaaatcat caggctggcc tgtacagtgt cagtccagct caccgcgagg
     2701 aaaccgccaa taaaggtgta aaggatcgtc gccgcagctc ccgcccacag agccgtttcg
     2761 tagctcatgc caaaggtact ttcaaacaga cgcgcgcctg ccacaatgcc cgaagcgcaa
     2821 taaatggtga agaacagcaa aatcaccagc gcggagataa tgcgcaaaat gcggctttta
     2881 tcttcaaaac gcccggtgaa ataatccggt aatgttaagg cgttattgtt gtattcggta
     2941 tgcacacgca accgcccggc caccagcttc cagttaatcc acgcgcctaa tgtcaggcca
     3001 atggcgatcc aactttcgga aatcccggaa agaaaaaccg cgcccggcaa ccccattaac
     3061 agccagccgc tcatatccga tgcacccgcc gataatgccg tcacgaatgg cccaaggcta
     3121 cgtccgccca gaatatagtc gtcaaagttt ttcgttgatc gccaggcgat aaacccaatc
     3181 aatatcatgc caaagatata gacacaaaat gtcaccaaca tcggtgtgct aatagccatc
     3241 taaagtctcc aaaaaattat tatcggcaat gtcgaaactt gccgttatat ctgccaccgg
     3301 aacggggtaa cagagtttat gttttaccag ggcgaccgta tcctgccgga agcgctggtt
     3361 attcacaatc gatttaacac accatttaca ttaaatttta gtgctcagcg acactatttt
     3421 tcatcaggtt gcactctctc acattttttg cggttgcacc tttcaaaaat gttaactgcc
     3481 gcagagaaaa agtctgagtt atttttttaa tccctgtcat atcgatttct tttattaaca
     3541 tttcattcat ttttaagctt gctacgcagg tcacatttaa catggttgca caaagttgca
     3601 acatcatgga tatttcacga taacgttaag ttgcaccttt cagaacaaca ggagtaatgg
     3661 catgggaacc accaccatgg gggttaagct ggacgacgcg acgcgtgagc gtattaagtc
     3721 tgccgcgaca cgtatcgatc gcacgccaca ctggttaatt aagcaggcga ttttttctta
     3781 tctcgaacaa ctggaaaaca gcgatactct gccagagcta cctgcgctgc tttctggcgc
     3841 tgccaatgag agcgatgaag caccgactcc ggcagaggaa ccacaccagc cattcctcga
     3901 ctttgccgag caaattttgc cccagtcggt ttcccgcgct gcgatcaccg cggcctatcg
     3961 ccgcccggaa accgaagcgg tttctatgct gctggaacaa gcccgcctgc cgcaaccagt
     4021 tgctgaacag gcacacaaac tggcgtatca actggccgat aaactgcgta atcaaaaaaa
     4081 tgccagtggc cgcgcaggta tggtccaggg gttattgcag gagttttcgc tgtcatcgca
     4141 ggaaggcgtg gcgctgatgt gtctggcaga agcgttgttg cgtattcccg acaaagccac
     4201 ccgtgatgcg ttaattcgcg acaaaatcag caacggtaac tggcagtcac acattggtcg
     4261 tagcccgtca ctgtttgtta atgccgctac ctgggggctg ttgtttactg gcaaactggt
     4321 ttccacccat aacgaagcca gcctctcccg ctcgctgaac cgcattatcg gtaagagcgg
     4381 tgaaccgctg atccgcaaag gtgtggatat ggcaatgcgc ctgatgggcg agcagttcgt
     4441 cacgggcgaa accatcgcgg aagcgttagc caatgcccgc aagctggaag agaaaggttt
     4501 ccgttactct tacgatatgc tcggcgaagc tgcactgact gccgcagacg cacaggcgta
     4561 catggtttcc tatcagcagg cgattcacgc cattggtaaa gcgtctaacg gtcgtggcat
     4621 ctatgaaggt ccgggcattt caatcaagct gtcggcgctg catccgcgtt acagccgcgc
     4681 ccagtatgac cgggtaatgg aagagcttta cccgcgtctg aaatcactca ccctgctggc
     4741 gcgtcagtac gatattggta tcaacattga cgccgaagag gccgatcgtc tggagatctc
     4801 cctcgatctg ctggaaaaac tctgtttcga gccagagctg gcaggctgga acggcatcgg
     4861 ttttgttatt caggcttatc aaaaacgctg cccgctggtg attgattacc tgattgatct
     4921 cgccacccgc agccgtcgcc gtcttatgat tcgcctggtg aaaggcgcgt actgggatag
     4981 tgaaatcaag cgcgcgcaga tggacggcct tgaaggttat ccggtttata cccgcaaggt
     5041 atataccgac gtttcttatc tcgcctgtgc gaaaaagctg ctggcggtgc cgaatctaat
     5101 ctacccgcag ttcgcaacgc acaacgccca cacgctggcg gcgatttatc aactggcagg
     5161 tcagaactac tatccgggtc agtacgagtt ccagtgcctg catggtatgg gcgagccgct
     5221 gtatgagcag gtcaccggga aagttgccga cggcaaactt aaccgtccgt gtcgtattta
     5281 tgccccggtc ggcacacatg aaacgctgct ggcgtatctg gtgcgccgcc tgctggaaaa
     5341 cggtgctaac acctcgtttg ttaaccgtat tgccgatacc tctttgccac tggatgaact
     5401 ggtcgccgat ccggtcactg ctgtagaaaa actggcacaa caggaagggc aaactggatt
     5461 accgcatccg aaaattcccc tgccgcgcga tctttacggt cacgggcgcg acaactcggc
     5521 agggctggat ctcgctaacg aacaccgtct ggcatccctc tcttctgccc tgctcaatag
     5581 tgcactgcaa aaatggcagg ccttgccaat gctggaacaa ccggtagcgg caggtgagat
     5641 gtcgcccgtt attaacccgg cggaaccgaa agatattgtg ggctatgtac gtgaagccac
     5701 gccgcgtgaa gtagagcagg cgctggaaag tgcggttaat aacgcgccaa tctggtttgc
     5761 cactcctccg gctgaacgcg cggcgatttt gcatcgcgct gccgtgctga tggaaagcca
     5821 gatgcagcaa ctgattggta ttctggtgcg tgaagccgga aaaaccttca gtaacgccat
     5881 tgccgaagtg cgtgaagcgg tcgattttct ccactactac gccggacagg tgcgggatga
     5941 tttcgctaac gaaacccatc gtccattagg gcctgtggta tgtatcagcc cgtggaactt
     6001 cccgctggcg attttcaccg ggcagatcgc tgccgcactg gcggcaggta acagcgtact
     6061 ggcaaaaccg gcagaacaaa cgccactgat tgccgcgcaa gggatcgcca ttttgctgga
     6121 agcgggcgta ccgccaggcg tggtgcaatt gctgccaggt cggggtgaaa ccgtgggcgc
     6181 gcaactgacg ggtgatgatc gcgtacgcgg ggtgatgttt accggttcaa ccgaagtcgc
     6241 tacgttactg cagcgcaata tcgccagccg cctcgacgct cagggtcgcc ctattccgct
     6301 catcgctgaa accggcggca tgaacgcgat gattgtcgat tcttcagcac tgaccgaaca
     6361 agtcgtaata gatgtgctgg cctcggcgtt cgacagtgcg ggtcagcgtt gttcggcgct
     6421 gcgcgtgctg tgcctgcaag atgagattgc cgaccacacg ttgaaaatgc tgcgcggcgc
     6481 aatggccgaa tgccggatgg gtaatccggg tcgcctgacc accgatatcg gtccggtgat
     6541 tgatagcgaa gcgaaagcca atattgagcg ccatattcag accatgcgta gcaaaggccg
     6601 tccggtgttc caggcggtgc gggaaaacag cgaagatgcc cgtgaatggc aaagcggcac
     6661 ctttgtcgcc ccgacgctga tcgaactgga tgactttgcc gaattgcaaa aagaggtctt
     6721 tggtccggtg ctgcatgtgg tgcgttataa ccgtaatcag ctgccggagc tgatcgagca
     6781 gattaacgct tccggctatg gtctgacgct tggcgttcat acgcgtattg atgaaaccat
     6841 cgcccaggtc actggctcag cccatgttgg taacctgtat gttaaccgta atatggtggg
     6901 cgcagtggtt ggggtgcagc cattcggcgg cgaagggttg tccggtaccg ggccgaaagc
     6961 aggcggtccg ctctatctct accgtctgct ggcgaatcgc ccggaaagtg cgctggcggt
     7021 gacactcgcg cgtcaggatg cagagtatcc ggtcgatgcg cagttgaaag ccgcattgac
     7081 tcagccgcta aatgcactgc gggaatgggc agcaaatcgt ccagaattgc aggcgttatg
     7141 tacgcaatat ggcgagctgg cgcaggcagg aacacaacga ttgctgccag ggccgacggg
     7201 tgagcgcaac acctggacgc tgctgccgcg tgagcgcgta ttgtgtatcg cggatgatga
     7261 acaggatgcg ctgactcagc tcgccgccgt gctggcggtg ggcagccagg tgctgtggcc
     7321 agatgacgcg ctgcatcgtc agttagtgaa ggcattgcca tcgactgtca gcgagcgtat
     7381 tcaactggcg aaagcggaaa atattaccgc tcaaccgttt gatgcggtga tcttccacgg
     7441 tgattcggat cagcttcgcg cattgtgtga agcagttgcc gcgcgggatg gcgcaattgt
     7501 ttcggtgcag ggttttgccc gtggcgaaag caatatcctg ctggaacggc tgtatatcga
     7561 gcgttcgctg agtgtgaata ccgctgccgc tggcggtaac gccagcttaa tgacgatcgg
     7621 ttaaacaggt agccggagga tgttacaccc tcctccggca tcattagcgt ggtcgaatcc
     7681 cctcaataat aatccgctgc acgttttcaa ccgtttgatt gaaaaatacc tcatcgcgca
     7741 acgttgcgcc tgtcaccgcc tccacctgag gggcgaaatc ggcgtaatgt tgagtggaag
     7801 cccaaatcat aaaaatcaaa tgctgcggat caatcggcgc gagtttgccg cttttgaccc
     7861 aaccggcaat cagcgccgat ttctcatcaa ttaatgactt caaatcgccc gtcagttcat
     7921 ccattaacag cggcgcgcct gccagcatct ccatacagaa caggcgcgaa gcctgcggat
     7981 aatcgcgtga gacttccagc ttcagacgga tgtactcttt gatcgccgcc agcggggcga
     8041 aatcttcacg aaacgctttt aacggtgcca gccagatatc gagaatctgc cgcagcacgg
     8101 caatatacag cgcctctttt gacggaaagt aatacagcag attggttttt gaaacacccg
     8161 ccaactctgc aatttgctcc agccgtgtgc cgtgaaaacc gaattgtgaa aaagtgtcca
     8221 gtgctgcgct aagaatcgct tttttcttcg cgcttactgc gcgcgaacgt ttacccgttg
     8281 ttttcactgc gccttgcgtc atgcgctctc ccctcttgta gattggcaac aggatagcaa
     8341 aacccccgcc cggcctcgac cttctgcact ctcatcgcgc acagatgcat gttttatgtg
     8401 caactgtttt gaccgtttag tccacttttt accagacatt ttagataacg cgatgttaac
     8461 acattaataa cactcccttt ttaaaaactg gcatccgctt tgcaaacaag ccaatacgcg
     8521 gctgaaaagg atatgcagaa tgcagcgccc cgtctgactt tcactttacg agacgaagag
     8581 aggttgatga tgaaaattgg cgtattcgta cctattggca acaacggctg gctcatttcg
     8641 acccacgcgc cgcagtacat gccgaccttt gaactgaata aagccatcgt gcaaaaagcg
     8701 gagcactacc atttcgattt cgccctgtcg atgatcaaac tgcgtggctt tggcggcaaa
     8761 actgagttct gggatcacaa ccttgagtcg ttcaccttga tggcggggct ggcggccgtg
     8821 acctcgcgca ttcagattta cgccaccgcc gccaccttaa cgttacctcc ggcaatcgtc
     8881 gcccgtatgg ccgcaaccat cgactccatc tctggcgggc gttttggcgt caacctcgtg
     8941 actggctggc aaaagcccga gtatgagcag atgggtatct ggcctggcga tgactatttc
     9001 tcccgtcgtt acgactatct caccgagtat gttcaggtgc tgcgcgacct gtggggcacg
     9061 ggaaaaagcg attttaaagg cgattttttc accatgaatg attgtcgcgt cagtccgcaa
     9121 ccgagtgtcc ccatgaaagt gatctgcgcc gggcaaagcg acgctggcat ggcgttctcc
     9181 gctcagtatg ccgatttcaa cttctgtttc ggcaaaggcg taaatacacc cacggctttc
     9241 gccccgaccg ctgcgcggat gaaacaggcc gcagagcaaa ccgggcgcga cgttggctct
     9301 tatgtattgt ttatggtgat tgccgatgaa accgacgatg ccgctcgcgc caaatgggaa
     9361 cactacaaag cgggcgcgga tgaagaggcg ttaagctggc taaccgaaca aagtcagaaa
     9421 gatacccgct ccggtactga caccaacgtc cgtcagatgg ccgatcccac ttcggcggta
     9481 aacatcaata tggggacgtt agtcggttct tacgccagtg tcgcgcgcat gttagatgaa
     9541 gtcgcaagcg tgcctggtgc cgaaggcgtg ctgttaacct tcgacgattt tctgtcggga
     9601 atcgaaacct tcggcgagcg cattcaacca ctgatgcagt gccgcgccca tctccctgtg
     9661 ctgactcagg aggtggcatg atgacgacat taaccgctcg accggaagcc attaccttcg
     9721 atccgcagca aagtgcgctg atcgtggtgg atatgcaaaa cgcctatgcc acgccaggcg
     9781 gctacttaga tctcgccggg tttgatgtct caaccactcg cccggtcatt gccaacattc
     9841 aaaccgccgt gaccgcagcg cgagcggcag ggatgttgat catctggttt caaaatggct
     9901 gggatgaaca gtatgtcgag gctggcggcc ctggctcacc gaattttcat aaatcgaacg
     9961 ccctcaaaac catgcgtaaa cagccgcagc tgcaggggaa attgctggcg aaaggctcct
    10021 gggattatca actggtggat gaactggtgc cgcagcctgg cgatattgtg ctgccgaagc
    10081 cgcgctacag cggtttcttc aatacgccgc tggacagcat tttgcgcagc cgcggaatac
    10141 gccatctggt tttcaccagc atcgctacca acgtctgcgt cgaatcgacg ctacgcgatg
    10201 gcttttttct ggagtatttc ggcgtggtgc tggaagacgc aacacaccag gcggggccgg
    10261 aatttgtaca gaaagccgcg ttgttcaata tcgaaacctt ttttggctgg gtcagcgacg
    10321 tcgaaacgtt ctgcgacgcg ctttctccca cgtcctttgc tcgtatcgct taaggagttt
    10381 aacgatgcca aaatccgtaa ttattcccgc tggcagcagc gcaccgctgg cccccttcgt
    10441 tcccggcacg ctggctgatg gcgtggtgta tgtctccggt acgctggctt ttgatcaaca
    10501 caataacgtg ctgtttgccg atgacccaaa ggcgcaaacc cgccacgttc tggaaactat
    10561 ccgcaaggta atcgagacag cgggtggcac gatggcggat gtgaccttca acagcatctt
    10621 tattaccgac tggaaaaatt acgccgcgat taacgaaacc tacgccgagt tttttccagg
    10681 tgataaaccg gcgcgattct gcattcagtg cggactggta aaacctgacg cgctggtgga
    10741 aatcgccaca attgcgcata tcgccaagtg aggccgcgat gaaactttca ctctcacctc
    10801 ccccttatgc tgatgcgccc gtagtggtgt tgatttcggg tcttggcggt agcggcagtt
    10861 actggttacc gcaactggcg gtgctggagc aggagtatca ggtagtctgt tacgaccagc
    10921 gcggcaccgg caataatccc gacacgctgg cagaagatta cagtatcacc cagatggcag
    10981 cggaactgca tcaggcgctg gtagccgcag ggattgagca ttacgcagtg gtcggccatg
    11041 cgctcggtgc gctggtggga atgcagctgg cgctggatca tcccgcgtcg gtaactgtgc
    11101 tggtctgcgt taacggctgg ctacgaataa acgcccatac gcgccgctgt tttcaggttc
    11161 gcgaacgatt actgtatagc ggtggcgcgc aggcatgggt ggaagcgcag ccgttgttcc
    11221 tctatcccgc cgactggatg gcggcccgcg cacctcgcct tgaggcagaa gacgcgctgg
    11281 cactggcgca ttttcagggc aaaaataatt tactgcgtcg acttaacgcc ctcaaacgcg
    11341 ctgactttag tcaccatgcg gatcgcatcc gctgcccggt gcaaatcatc tgcgccagtg
    11401 atgatctgct ggtgccatca gcatgttcca gtgaacttca tgccgccctg cccgatagcc
    11461 agaaaatggt gatgcgctat ggcggacacg cctgcaacgt gaccgatccc gaaacgttta
    11521 atgctctgtt actcaacggg cttgccagcc tgttacatca ccgtgaagcc gccctgtaag
    11581 gaattgctat gaacgaagcc gttagcccag gtgcgcttag caccctgttc accgatgccc
    11641 gcactcacaa cggctggcgg gagacacccg tcagcgatga gacgttacgg gagctttatg
    11701 ccctgatgaa atgggggccg acatcagcta actgttctcc ggcacgtatc gtgtttatcc
    11761 gcactgcaga gggaaaagaa cgtctgcgcc cggcgctttc cagcggcaat ctgcaaaaaa
    11821 ccctgaccgc gcccgtcacc gctatcgtcg catgggacag tgaattttat gaacggttac
    11881 cgcaactgtt tcctcacggc gatgcccgca gttggtttac ctccagccca caacttgccg
    11941 aagaaacagc gtttcgcaac agttccatgc aggcggctta tctgatcatc gcctgccgtg
    12001 cgctggggct ggataccggc ccgatgtcgg gttttgatcg tcaacacgta gacgacgctt
    12061 tttttgcagg cagcacgctg aagagcaatc tgctgattaa tatcggctat ggcgatagca
    12121 gcaaactttt tgcgcgcctg ccacgtctgt cctttgaaga agcctgcggg ctgttgtaag
    12181 gagcaaacta tgaacattgt cgatcaacaa acttttcgcg atgcgatgtc ctgcatgggc
    12241 gcggcggtca atatcatcac cacggacggt ccagccgggc gcgccgggtt caccgccagc
    12301 gccgtctgca gcgtgaccga cacgcctccc acattactgg tgtgcctgaa tcgtggggcg
    12361 tccgtctggc cggtattcaa tgaaaatcga acgctgtgtg taaatacact tagcgccggg
    12421 caggagccgc tttcaaacct ttttggcggc aaaacgccca tggaacaccg ctttgccgcc
    12481 gcccgctggc agactggtgt gaccggatgc ccgcaactgg aagaggcgct ggtttcgttt
    12541 gactgccgta tcagccaggt ggtgagcgtc ggcacccacg acattttgtt ttgcgccatc
    12601 gaagcgattc atcgtcacgc caccccctac gggctggtgt ggttcgatcg cagttatcac
    12661 gcgctgatgc gccctgcttg ttaacctcct taaggagaca gctgatggca atgttcggtc
    12721 ttcctcactg gcagttaaaa tcgacctcta cagagagcgg cgtggtcgcg ccggatgaac
    12781 gattgccgtt tgctcagacg gcagtaatgg gcgttcaaca cgcggtggcg atgtttggcg
    12841 cgacggtgtt aatgccgatt ctgatggggc tggatcccaa tctttccatt ttaatgtcag
    12901 ggatcggcac gttactgttt ttctttatta ccggcgggcg cgtgcccagt tatctcggct
    12961 ccagcgcggc ttttgtcggc gtggtgatcg ccgcaacggg ctttaacggt cagggcatta
    13021 acccgaatat cagcattgcg ctgggcggga ttatcgcctg tgggctggtt tataccgtca
    13081 ttggcctggt ggtgatgaaa atcggtacgc gctggattga acggctaatg ccgccggtgg
    13141 tgacgggtgc ggtggtgatg gcgattggcc tgaacttagc gcctatcgcg gtaaaaagtg
    13201 tatcggcttc aggctttgat agctggatgg cggtaatgac ggtgctgtgt attgggctgg
    13261 tggcggtgtt tacccgtggc atgatccagc ggttattgat tcttgtagga ttaattgtcg
    13321 cctgtctgct gtatggcgtg atgaccaacg ttttagggct gggaaaagcc gtcgatttta
    13381 ctctcgtcag ccatgctgcc tggttcggtc tgccacattt ttccactcct gcttttaatg
    13441 gtcaggcaat gatgctgatt gcgcccgtgg cggtgattct ggtggcggaa aacttaggtc
    13501 atctcaaggc ggtcgccggg atgaccggac gcaatatgga cccgtatatg gggcgagcat
    13561 tcgtaggcga tggtctggca acgatgcttt ctggttctgt cggcagtagc ggcgtcacca
    13621 cctatgctga aaatatcggt gtgatggccg tgacaaaagt ctactcgacg ctggtgtttg
    13681 ttgctgctgc cgtcatcgcc atgctgttgg gcttttcacc gaaatttggc gcgctgattc
    13741 ataccattcc ggccgcggtg attggtggtg catcaattgt tgtgttcggg ctgattgccg
    13801 tcgcaggcgc aaggatatgg gtacaaaacc gtgtcgattt aagccagaac ggtaatttga
    13861 ttatggtcgc agtaacctta gtgctgggcg cgggtgattt tgctctcacg ctgggcggtt
    13921 ttacgttggg aggaatcggt acagcaacct ttggcgcgat tttactcaat gcgttattaa
    13981 gcagaaggtt ggttgacgtt ccaccgccag aagttgttca tcaggagcca taaacagaaa
    14041 accgccgggg ttaccggcgg tgatggcttt ttgacgagct taagaaaaaa atgcattcgt
    14101 cattgattag cccggctagt tgtcgctttt gccgtgactg ctcttgccac cttttttacc
    14161 tgcttctgat gctctttcgc ggtcttctgc aaaattgccg gaaccgcctc gatggtttgc
    14221 cattactgac ctccgtctgg gtatagacat catattgcta gaattaacgc ggcgtatggc
    14281 ggctgccata ttttcgctac gtgggactaa gcttagtgaa ctgacgtgtt tctacccgcg
    14341 tttggtaata tcctgcaaca gcttcttagc gagaatagat atcatctcca ataattatcc
    14401 ataagccgct cttatgatta aaaaaacgcg atgagataaa aatgtgtcat tttgcgacaa
    14461 aattgcgtgc ttgtgaaagt tgttataaat caaataagtg gttgtgaaat ttgcactctg
    14521 aaaaggacgt cttatcttta aataagtggt agcgaatcgc tacggaatag agataacacg
    14581 aggagtggtt agaaatggct aaagttctgg tgctttatta ttccatgtac ggacatattg
    14641 aaacgatggc acgcgcagtc gctgagggtg caagcaaagt ggatggcgct gaagttgtcg
    14701 ttaagcgtgt accggaaacc atgtcgccgc aattatttga aaaagcaggc ggtaaaacgc
    14761 aaactgcacc ggttgcaacc ccgcaagaac tggccaatta cgacgccatt atttttggta
    14821 cacctacccg ctttggcaac atgtccggtc aaatgcgtac cttcctcgac cagacgggcg
    14881 gcctgtgggc ttccggcgca ctatacggaa aactggcgag cgtctttagt tccaccggta
    14941 ctggcggcgg tcaggaacaa accatcacat ccacctggac gacccttgcg catcacggca
    15001 tggtgattgt ccccattggc tacgcagcgc aggaattatt tgacgtttca caggttcgcg
    15061 gcggtacgcc gtacggcgca accaccatcg caggcggtga cggttcacgc cagcccagcc
    15121 aggaagaact gtctattgct cgttatcaag gggaatatgt cgcaggtctg gcagttaaac
    15181 ttaatggcta atcttcaaca ggaggatacg catgccaact caagaagcga aagctcatca
    15241 cgtcggtgaa tgggcatctc tgcgcaatac gtcgccggaa atagccgagg ccattttcga
    15301 agtcgccggg tacgacgaaa aaatggcgga aaagatttgg gaagaaggta gcgatgaagt
    15361 gttagtcaaa gcctttgcta agaccgacaa agattcgctt ttttggggcg aacagaccat
    15421 cgaacgtaaa aacgtttaac cagcgactcc cccgtagcga acgggggagt tttctgttat
    15481 ttcaccgctt cattcaacac gctatcaaac ttatccatcg ggcagaaacc atcagcgtct
    15541 atcgggcaac cgcttaattc cagcgtcacg cgctgcgcag gtgcctgcag ggttaacgca
    15601 tcggcattac gtaactgttc cgcactctgg tacacatatt caattttcat caaatcgcga
    15661 ttggctttgc tgtcacgcca acgctggaaa acgattttgc cgccaatcgg cgtgcgttcg
    15721 ttctggtcat gcaactgata cggtttgaaa tccagcgccg ttaacagaga ggcaatgttg
    15781 gagtcatgcc caactaacac cgtgattttt ggagcactgg tgcgatcggt gaccagggct
    15841 ttgtcgatat aactgaccag cggtttcgca acattgcgcg ccacttccgg tgaggtaaac
    15901 aggctgtcct ggtaaccgtt tttcagcttc gacaacacct tccactgctg gtcagatttg
    15961 atttctcccc aggccacctg atccatcgga aaaccttcgt agtattgcaa agtaaacgca
    16021 tctaccagcg agttgccgac tttcagcggc ccggaaacac ctggttcttg ttgatacttg
    16081 gcgctaaagg tatttttgcc atccaccagc gaacactgtt gtttctcttt acaggcaggg
    16141 gaatctttat agttaacgat tttttccagt agctggtagc tgtcggtaag ctggagtttg
    16201 ctgagctctt tctccattgc cgccaccgcc tgttcactga atgcggcgga atcatcggtg
    16261 atcaccgggt taaaggttgg gtccatagtg cccatttttt cctggtgatg cacaggaata
    16321 tcacaccccg ggaatgcgcc ggtaataaag aactgtgcgg tcgcaacggt acgttgcaga
    16381 ctattggcat aggcataaac ggtgtccggc ggcgggcatt cccccgattt taccatcccc
    16441 tgctctgcca gccattcacg catgtaatgg cccatataca cttcgagtac gccacctttg
    16501 gtggtgagtt gcccaccggg gacgtcccat tcgggccatt tattcggcgt cgactgctcc
    16561 agcacactgc cattgttcgc cagcggcgca cgtaagttat ggcggctcat catgagcact
    16621 tgctgtagct gatagccttc cggtacggtt tgtgcctgag cgtttgaagc gagtaaaact
    16681 acccctgcca cagctgcggc gattagcgtt ttgttcattc ctggcacctc ttttgttatc
    16741 aatcactaaa gagtgtgaca gaaatatgac ttttttccgg gaacaggttc gcaaaatggc
    16801 ttgttgaacg gctgttttta tattcatctt taaaaacagg caaggtttga cgcaaactat
    16861 gcctgattag cccctgcctc gcgtacgccg ctgatgattg cacctgtggc taaacagaat
    16921 atcggcggcg ttaccaatac agaggcaata tttaccaata aaaaagtgtg agactaataa
    16981 caatggtttt tagcatatgc ctctgtcatt aaattgcata acgagttaaa aaaccaattt
    17041 gcgcattcct ttccttattg tttaactcgt aagcctgagc aaaaagttca ggagggaggt
    17101 aaacgtcata atgattgcca gtgtttatta aataaccata ctcaagatct tcatctgttg
    17161 aaaaggattc attccctaag gagattgatg ccacattact attaacaata aataatgagc
    17221 aaatgctatc tgaaagagca gtgaggaaat ttttcacctc attaatcctc ttctctgggt
    17281 cggtgtctaa aacaaggcgc gccttgttat cattaaatat aaactcgggt aaatgcagta
    17341 tgcatttttt gtcattcaag caactatcaa taaattcttt ttttaaaaaa cagaaagttc
    17401 catgacataa gactaccctg cccctgaatt ttgttgtcag attgtagatc aacgccaaat
    17461 aacgtcttta catcttccct gagattatag aatgccgtat caacaagagc ctcgatcgtt
    17521 tgtttatcaa caattttaat aaactcttcg tatacttgct tttgcgtctt atggctgccc
    17581 agcgcccatg aatactcatg ttcttttaaa accagtatgg cccttaacag acaatagcca
    17641 tcgccatcta ctggagcgta ttttaatcca taagttttat taatttcact ctgaataaaa
    17701 gatataccag acaaatcaat accagaatct aaatcagata ttcccggcgt atttatccat
    17761 tctggctgga tactcatatt ttcatcatta tcaaaacaat aatcagaata aacatcatca
    17821 cacgcattaa tgttttcaat atattccata aagccatacc aataataacc tttaaaatct
    17881 gtgatagact ctgaacctct acccggcaag gatactctaa caatatcaac atcttca
//
LOCUS       DAZKIP010000090        17860 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZKIP010000090 DAZKIP010000000
VERSION     DAZKIP010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17860
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.090"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(560..1756)
                     /gene="hemY"
                     /locus_tag="ACHOUK_003303"
     CDS             complement(560..1756)
                     /gene="hemY"
                     /locus_tag="ACHOUK_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFP9898692.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1759..2970)
                     /gene="hemX"
                     /locus_tag="ACHOUK_003304"
     CDS             complement(1759..2970)
                     /gene="hemX"
                     /locus_tag="ACHOUK_003304"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFP9898693.1"
                     /translation="MTEQEKTSAVVEETKEAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKVELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQQETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPQ
                     ADTPAAAPQGE"
     gene            complement(2992..3732)
                     /gene="hemD"
                     /locus_tag="ACHOUK_003305"
     CDS             complement(2992..3732)
                     /gene="hemD"
                     /locus_tag="ACHOUK_003305"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFP9898694.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3729..4670)
                     /gene="hemC"
                     /locus_tag="ACHOUK_003306"
     CDS             complement(3729..4670)
                     /gene="hemC"
                     /locus_tag="ACHOUK_003306"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFP9898695.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            5057..7603
                     /gene="cyaA"
                     /locus_tag="ACHOUK_003307"
     CDS             5057..7603
                     /gene="cyaA"
                     /locus_tag="ACHOUK_003307"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFP9898696.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7643..7963)
                     /gene="cyaY"
                     /locus_tag="ACHOUK_003308"
     CDS             complement(7643..7963)
                     /gene="cyaY"
                     /locus_tag="ACHOUK_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFP9898697.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFRDLLEQAATQQAGET
                     VSFR"
     gene            complement(8125..8267)
                     /locus_tag="ACHOUK_003309"
                     /pseudo
     CDS             complement(8125..8267)
                     /locus_tag="ACHOUK_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            8426..8629
                     /gene="yifL"
                     /locus_tag="ACHOUK_003310"
     CDS             8426..8629
                     /gene="yifL"
                     /locus_tag="ACHOUK_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP9898698.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            8666..9490
                     /gene="dapF"
                     /locus_tag="ACHOUK_003311"
     CDS             8666..9490
                     /gene="dapF"
                     /locus_tag="ACHOUK_003311"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFP9898699.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            9487..10194
                     /gene="yigA"
                     /locus_tag="ACHOUK_003312"
     CDS             9487..10194
                     /gene="yigA"
                     /locus_tag="ACHOUK_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HFP9898700.1"
                     /translation="MKQPGEELQETLTELDDRVVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            10191..11087
                     /gene="xerC"
                     /locus_tag="ACHOUK_003313"
     CDS             10191..11087
                     /gene="xerC"
                     /locus_tag="ACHOUK_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFP9898701.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQNWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKTPRHLPKNIDVDDINRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNALSWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            11087..11803
                     /gene="yigB"
                     /locus_tag="ACHOUK_003314"
     CDS             11087..11803
                     /gene="yigB"
                     /locus_tag="ACHOUK_003314"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFP9898702.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEQEALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            11887..14049
                     /gene="uvrD"
                     /locus_tag="ACHOUK_003315"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             11887..14049
                     /gene="uvrD"
                     /locus_tag="ACHOUK_003315"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFP9898703.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(14094..14996)
                     /locus_tag="ACHOUK_003316"
     CDS             complement(14094..14996)
                     /locus_tag="ACHOUK_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HFP9898704.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSIV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVREIDEKANRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSRQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCDK"
     gene            complement(15079..15843)
                     /gene="yigE"
                     /locus_tag="ACHOUK_003317"
     CDS             complement(15079..15843)
                     /gene="yigE"
                     /locus_tag="ACHOUK_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFP9898705.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            16005..16061
                     /gene="ysgD"
                     /locus_tag="ACHOUK_003318"
     CDS             16005..16061
                     /gene="ysgD"
                     /locus_tag="ACHOUK_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFP9898706.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            16213..17163
                     /gene="corA"
                     /locus_tag="ACHOUK_003319"
     CDS             16213..17163
                     /gene="corA"
                     /locus_tag="ACHOUK_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFP9898707.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(17205..17696)
                     /locus_tag="ACHOUK_003320"
     CDS             complement(17205..17696)
                     /locus_tag="ACHOUK_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HFP9898708.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCITKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIEQVFNPHNFKK
                     DNF"
     gene            complement(17693..>17860)
                     /locus_tag="ACHOUK_003321"
     CDS             complement(17693..>17860)
                     /locus_tag="ACHOUK_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HFP9898709.1"
                     /translation="VAQDHFGLDVTDIKNKTYSKYRFFRIWFFLQRHKDFAFKPFFTN
                     FNTIERIENYL"
BASE COUNT         4130 a         4589 c         4708 g         4433 t
ORIGIN      
        1 tatccaagaa atgaatttaa aaatcaggag agcgttttca atcctacctc tggcgcagtt
       61 gatatgtaag gcaggtttat tatatcgcgt tgattattga tgctgttttt agttttaacg
      121 gcaattaata tatgtgttat taattgaatg aattttatca ttcataataa gtatgtgtag
      181 gatcaagctc aggttaaata ttcactcagg aagttattac tcaggaagca aagaggatta
      241 cagaattatc tcataacaag tgttaaggga tgttatttcc cgattctctg tggcataata
      301 aacgagtaga tgctcattcc atctcttatg ttcgccttag tgcctcataa actccggaat
      361 gacgcagagc cgtttacggt gcttatcgtc cactgacaga tgtcgcttat gcctcatcag
      421 acaccatgga cacaacgttg agtgaagcac ccacttgttg tcatacagac ctgttttaac
      481 gcctgctccg taataagagc aggcgttttt ttatgtgtca ggaaggtccc ggaggtgctt
      541 gcctccgggt gagaaggaac tactgtggtg ggttgttctg caacgttaac atcaaaccgt
      601 cgcgacgcat cgctgcagct tcttccggct tgtgcagtct gtccagcgcg tcggcaagcc
      661 atgcgtaatc gtaggcgtcc ggacgttgtt tcagcgctgc gcggaaggcg agcgatgctt
      721 cctgccattc tccgtgcttc atcagtgact ggcccagtgt gctccacaac agcgggcgat
      781 cgccgacgtt tttgatttgc tggcgcagca ctttttccag ctgttccgga ttgtttgttt
      841 tcagtcgagg aatcggcagc agtaggcgat cgtcgtactg gcgtttcagg ccatcgatga
      901 taatttgctg ggcagtatca tgatcgtcac attcaataag atgttccgcc attgccacct
      961 gcaacgctac ctgatgacgc gttttccggc tttggttttt ccaccagtta cgcaaacctt
     1021 cgctaccgtt atcggcacgc gcctgatcca tcaggccaat ccatgcctgt tgttccagca
     1081 ttgcacgatg ttcttcatca ccaacatggg ctttcgccat tgatgggata atatccagca
     1141 gcgaactcca tgcacctgtg cggatatacg cctgttccgc cagacgtaat acttccggat
     1201 ggcgtggcgt aacttccagc agcttatcca cgccgtggcg tgcagcatgg ttttcattac
     1261 gggccagttg cagacgtaca cgggtgattt ctaccggaat ggtgtcattg ccggccagct
     1321 ccgctgcgcg ttccagatgt tggttggcgc gcgcttcatc accacgttgt tgcgcggctt
     1381 cggcagccag tagatagttc accaccggtt gttcagcgtg atcggcattt ttcgccatca
     1441 gcttttcaac ttgctgataa tcgccttccg ccagtttcag cagcgcctgt tcggtctgct
     1501 tacgtgcacg gcgacgctta cgcccgacaa accacccacg ggtgtgcgcg ccagtgcgga
     1561 agatccgccg cagtagccac tcaatggcaa acagcactac catcgccaga atcaatatga
     1621 tcgccaggcc cgtgacgctg gtttcgatat tgtagttgtc ggtctggatc agcacataac
     1681 cctgatgacc ggcaatcatc gggccaacca cgatccccgc aatcagcaac acaaagagca
     1741 ataacacttt tagcatggtt attctccttg cggcgcggct gccggagtat cagcttgcgg
     1801 tgcaggtgca ggtgcaggtg caggtgcagg tgcaggtgca ggtttagctt ccgttgtccc
     1861 cgctgccggt tgtgccagca ggttacgcac gcgagtctgc atcagttttt ccagcatcgc
     1921 ctggctttgc agggtttccg gaagatccat cgagatattt tgctggctta actggtctac
     1981 ctcgtcgagg aacgctttgg tggtggcatc atcagtatcg tagtaagcac gtacccaggt
     2041 ggagacgttc tccagcgcct ggcgataagt ctcttgctgg tgacgcggta cagcttgtgc
     2101 tgcgaccagc aggcgagagc gaatattttc gcgcagatag atatcctgat ttggcgctaa
     2161 cagcggtacg gcggtgtcat cacgacggcg aatcgtaatg aagttgtcca taaagttctg
     2221 ccagcttttt tgcagattga tacgccattc gctgatggaa ctggaaagct cttcaccgtc
     2281 ggaatccatc ggcgaaccat cgctgtcatt atcggccaga cgcaggttat ctacctgatt
     2341 tgaaagctga ttaagcttaa ggatgatgcc gtcataatcc acctgcgata ctgcagaaag
     2401 gctggcgata tcatcggtaa ttgcccgacg aacggtaatc agactcgggt cattcatatc
     2461 cgccaggctg gcgtctgcac ttttcagcaa cgctgcagcg gtcgtgacgt cctgatcgct
     2521 ccacagcttc cgtccggcga gtttcaccag aaaatcggcc tgagccagca gccaggtttt
     2581 agcatcgctg ccggaaatgg tggcgacctt ttgttggact tcatccaact gttttgccag
     2641 cgtttcttgc tgacgattcg cctgcttaag ctgtgcagct tgttgcttaa taatgccttc
     2701 cagctcgact ttttggctct cctgggcttt ttgcaatgcc gtcagttggt tagccagggc
     2761 atcgctggtg gcggtctgat tgacggcctg ttgtttaccc cagccataca aaccgacgcc
     2821 cgccgccaga gcaatagcga tagccaccgc gctgagaatc aatgcggtat tgttcttact
     2881 ctttttttct gttgcgacag gttgtgacgt ggtgtccacg gcctccttgg tctcttcaac
     2941 cacggcggag gttttttctt gttccgtcat tatggcttcc tgttatgaga gttattgtaa
     3001 tgcccgtaaa agcgcatcgt tgtcagcgtt atcggcgacc ttaatgtctt gccagcccag
     3061 ttcccgggcg agtttcgcca aacgctcact gacgaccaat agtcgacagt gtagtaacca
     3121 gtgctcacga taccattgtg ggatcagcga ccagagttgc tgcaacattt caccgctggt
     3181 aacaacgacc gtcgtcacct cgcgggattg ccagcgcatc gcttcttctg caccatcgta
     3241 atggattgcg catcgttgat aacattcaca aaaagtgacc tcagcaccgc gcgccgtcag
     3301 ggtatcccca attagctcac gaccgccatt gccacgtaat atcagcgcac gtttgcccgc
     3361 aatattttgt aattcaggta attgtagcaa gacttcgctg atttcccgat cctgcgggta
     3421 gagaatcttc tgtccgctta cggtatgtag tgccagtgcg gtggtgcgtc caatggcgaa
     3481 ataattaggt agtcggggcc atttacgatc ttgctgatgt agctgtgatt gggcaaaagc
     3541 aaccgcgtgt tgcgagaggg caaacaacag atcgctctcc cccagcgctg ccagttgatc
     3601 agcaagttgc ggtaattgtc gacccggaga aaactcaatc agcggaaaat gccaggccac
     3661 ctgccccagt gtgcgcagac ggctcactaa ctcttctcca gcgggagacg ggcgggtgac
     3721 aaggatactc atgccggggc gtctccgtta tagacttcag cgaggatctc gcgcgcgccg
     3781 ttattcagta gctcttctgc cagcgaaatc cccatttgtt cggcatcttg cggcgcaccg
     3841 cggcgttcac cgcgaataat ctgcgaaccg tccggcgcgc cgaccagcgc acgcagccag
     3901 atttcgccat caataagctc ggcgtagcta ccaattggca cctgacatcc accttcgaga
     3961 cgggtattca tggcgcgttc tgcggtaacg cgcagtgcag tttcgtggtg attcagcgcg
     4021 gcaagcagct cgcgagtgcg tgtatcatca aggcggcatt caataccgac tgcaccttgt
     4081 cctaccgccg gaagagaaat ctcgggtggc aacgcggcgc gaatgcggga ctccagacct
     4141 aaacgtttta gtccggctac ggcaagaatg atggcatcgt attcgccgtt atccagtttg
     4201 ctcaggcgag tgccgacgtt gccgcgcagg gagcggataa tcagatccgg gcggcgttca
     4261 gccagttggc actggcgacg taaactggac gtcccgacga tactgcctgc cggtaacgca
     4321 tccagactgt catagttatt ggacacaaag gcatcgcgag gatcttcacg ctcacaaata
     4381 gtgaccaggc ccagaccttg cgggaattca accggcacat ctttcattga atgtacggcg
     4441 atatcggcgc gattttcgag gagcgcgact tccagctctt ttacaaataa gccttttccg
     4501 ccgactttcg ccagcggcgt atcaagaatc acatcgccgc gcgtcaccat cggtaccagt
     4561 tcaacgacca ggcccggatg gctcgccatc aacttgtctt tgacatagtg tgcctgccag
     4621 agtgcaagtg ggctttggcg tgtggcaatt cttaaaacat tgtctaacat gcttgttacc
     4681 gtcattatca tccgtggtcc atcctaacat ccttgccaga gtgatgtcag tgttgtggtg
     4741 aaacgtagac gcctgcgcaa accgtaaaat gaggtctggc agtggatcct gacaggcgtt
     4801 tcacgccgtc gtaataagga atttacagag aataaacggt gctacacttg tatgtagcgc
     4861 atctttcttt acggtcaatc agcaaggtgt taaattgatc acgttttaga ccattttttc
     4921 gtcgtgaaac taaaaaaacc aggcgcgaaa agtggtaacg gttacctttg acatacgaaa
     4981 tatcccgaat gccgcgtgtt accgttgatg ttggcggaat cacagtcatg acgggtagca
     5041 aatcaggcga tacgtcttgt acctctatat tgagactctg aaacagagac tggatgccat
     5101 aaatcaattg cgtgtggatc gcgcgcttgc tgctatgggg cctgcattcc aacaggtcta
     5161 cagtctactg ccgacattgt tgcactatca ccatccgcta atgccgggtt accttgatgg
     5221 taacgttccc aaaggcattt gcctttacac gcctgatgaa actcaacgcc actacctgaa
     5281 cgagcttgaa ctgtatcgtg gaatgtcagt acaggacccg ccgaaaggtg agcttccaat
     5341 tactggtgta tacaccatgg gcagcacctc gtccgtaggg caaagttgtt cctctgacct
     5401 ggatatctgg gtctgtcatc aatcctggct cgatagcgaa gagcgccagt tgctacaacg
     5461 taaatgcagc ctgctggaaa gctgggccgc ctcgctgggt gtggaagtta gcttcttcct
     5521 gattgatgaa aaccgttttc gccacaacga gagtggcagt ctggggggcg aagattgtgg
     5581 ctccacccag catatactgc tgcttgatga attttatcgt accgccgtgc gtctcgccgg
     5641 taagcgtatt ctgtggaata tggtgccgtg cgacgaagaa gagcattacg atgactacgt
     5701 aatgacgctc tacgcacaag gcgtgctgac gccaaatgaa tggctggatc tcggcggctt
     5761 aagctcgctt tccgctgaag aatacttcgg tgccagcctt tggcaactct acaagagtat
     5821 cgattctcca tacaaagcgg tgctgaagac actgctgctg gaagcctatt cctgggaata
     5881 cccgaaccca cgcctgctgg cgaaagatat caaacagcgt ttgcacgacg gcgagattgt
     5941 atcgtttggc ctcgatcctt actgcatgat gctggagcgt gttactgaat acctgacggc
     6001 gattgaagat ttcacccgtc tggatttagt acgtcgctgc ttctatttaa aagtgtgcga
     6061 aaagctcagc cgtgaacgcg cctgcgtagg ctggcgtcgc gcagtgttga gccagttagt
     6121 gagcgagtgg gggtgggacg aagctcgtct ggcaatgctc gataaccgcg ctaactggaa
     6181 gattgatcag gtgcgtgagg cgcacaacga gttgctcgac gcgatgatgc agagctaccg
     6241 taatctgatc cgctttgcgc gtcgcaataa ccttagcgtc tccgccagtc cgcaggatat
     6301 cggcgtgctg acgcgtaagc tgtatgccgc gtttgaagca ttaccaggta aagtgacgct
     6361 ggtaaacccg cagatttcac ccgatctctc ggaaccgaat ctgaccttta tttatgtgcc
     6421 gccgggccgg gctaaccgtt caggttggta tctgtataac cgcgcgccaa atattgagtc
     6481 gatcatcagc catcagccgc tggaatataa ccgttacctg aataaactgg tggcgtgggc
     6541 atggtttaac ggcctgctga cctcgcgcac ccgcttgtat attaaaggta acggcattgt
     6601 cgatttgcct aagttgcagg agatggtcgc cgacgtgtcg caccatttcc cgttgcgctt
     6661 acctgcaccg acaccgaagg cgctctacag cccgtgtgag atccgccatc tggcgattat
     6721 cgttaacctg gaatatgacc cgacagctgc gttccgcaat caggtggtgc attttgattt
     6781 ccgtaagctg gatgtcttca gctttggcga gaatcaaaat tgcctggtag gtagcgttga
     6841 cctgctgtat cgcaactcgt ggaatgaagt gcgtacgctg cacttcaacg gcgagcaatc
     6901 gatgatcgaa gccctgaaaa ctattctcgg caaaatgcat caggacgccg caccgccaga
     6961 tagcgtggaa gtcttctgtt atagccagca tctgcgcggc ttaattcgta ctcgcgtgca
     7021 gcaactggtt tctgagtgta ttgaattgcg tctttccagc acccgccagg aaaccgggcg
     7081 tttcaaggcg ctgcgcgttt ctggtcaaac ctggggcttg ttcttcgaac gcctgaatgt
     7141 atcggtacag aaactggaga acgccatcga gttttatggc gctatttcgc ataacaaact
     7201 gcacggcctg tcagtgcagg ttgaaaccaa tcacgtcaaa ttaccggcgg tggtggacgg
     7261 ctttgccagc gaagggatca tccagttctt tttcgaagaa acgcaagatg agaatggctt
     7321 taatatctac attctcgacg aaagcaatcg ggttgaggtg tatcaccact gcgaaggcag
     7381 caaagaggag ctggtacgtg acgtcagtcg cttctactcg tcatcgcatg accgttttac
     7441 ctacggctca agcttcatca acttcaacct gccgcagttc tatcagattg tgaaggttga
     7501 tggtcgtgaa caggtgattc cgttccgcac aaaatctatc ggtaacatgc cgcctgccaa
     7561 tcaggatcac gatacgccgc tattacagca gtatttttcg tgatgaacgt gccggaaagc
     7621 gaggcttatc cggcatgcaa tcttagcgga aactgactgt ttcacccgcc tgctgcgtcg
     7681 ccgcctgttc cagcaaatcc cggaaggttt cgccgctgcg atcgcaaatc cactcatcgc
     7741 ctttcagatc aaaatggtag ccgccttgtt tggttgccag ccatacctgg tgcagcggtt
     7801 cctggcggtt gataatgatt ttgctgccat tctcaaaggt aatggtcagc acgccgccgt
     7861 tgatttcgca gtcgatatcg ctgtcgccat cccagtcgtc cagacgctct tcaatggtca
     7921 gccacagttg atcagccagg cgatgaaatt cactgtcgtt cattgttgta tcctgttttt
     7981 aagtgatggc ggcagtatag cggcatgggg tcagggcttc aaagtttgca cctctgcggc
     8041 tgcgttacgg catgattcat ccgtcaccgg aataatgatg tctctgtgta gcgaaagatt
     8101 tgtctcttca ttagggcgca gttacaccac gcctttccct gtttctggta aacattatga
     8161 tcaggtttac cgagcgagca tcctcacgct gacggaacta aaaaagccaa caaacaaacc
     8221 acattgcgat agtgcataaa gccatcctgg cgtgaggtgt tgatcacgaa actgccagca
     8281 gaacataaat cctcacgagt aagcgttata ctcgcagcat ttcctcactt ttcagacttc
     8341 ataaagagtc gctaaacgct tgcttttacg ccttctcctg cgatgataga aagcagaaag
     8401 cgatgaactt tacaggcaat ccataatgaa aaacgtgttt aaggcactca ctgtattact
     8461 tactctcttc agcctgacgg gctgcggtct gaaaggtccg ctctatttcc cgcctgcaga
     8521 taaaaatgca ccgccgccga ccaaaccggt agagacgcaa acgcaatcca cggtgccgga
     8581 taaaaacgat cgcgccactg gcgatggtcc atcccaggtg aattactaaa agtcagtttc
     8641 tgtacccgcg tgattggagt aaatgatgca gttctcgaaa atgcatggcc ttggcaacga
     8701 ttttatggtc gtcgacgcgg taacgcagaa tgtctttttt tcaccggagc tgattcgtcg
     8761 cctagctgat cggcacctgg gggtagggtt tgaccaactg ctggtggttg agccgccgta
     8821 tgatcctgaa ctggattttc actatcgcat tttcaatgct gatggcagtg aagtggcgca
     8881 gtgcggcaac ggcgcacgct gctttgcccg ttttgtgcgt ctgaaagggc tgaccaataa
     8941 gcgcgatatc cgcgtcagca ccgccaacgg gcggatggtt cttaccgtca ccgatgatga
     9001 tctggtccgc gtaaatatgg gcgaacccaa cttcgaacct tctgccgtgc cgtttcgcgc
     9061 taacaaagcg gaaaagacct atattatgcg cgccgccgag cagacaatct tatgcggcgt
     9121 ggtgtcgatg ggaaatccac attgcgtgat tcaggtcgat gatgtcgata ctgcggcggt
     9181 agaaacactg gggcctgttc tggaaagcca cgagcgtttt ccggagcgcg ccaatatcgg
     9241 ttttatgcaa gtggttaagc gcgagcatat tcgtttacgc gtttatgagc gtggggcagg
     9301 agaaacccag gcctgcggca gcggcgcatg tgcggcggtt gcagtaggga ttcagcaagg
     9361 tttgctggcc gaagaagtac gcgtggaact cccaggcggt cgtcttgata tcgcctggaa
     9421 aggtccgggt cacccgttat atatgactgg cccggcggta catgtctacg acggatttat
     9481 tcatctatga agcaaccagg ggaagaactg caggaaacac tcacggagct tgatgaccgg
     9541 gtggttgtcg attatctgat taaaaatcct gagtttttta tccgcaatgc gcgcgcagta
     9601 gaagcgatac gtgtgccgca tccggtacgc ggtaccgttt cgttggtcga gtggcacatg
     9661 gcccgcgcac gtaatcatat tcatgttctg gaagagaaca tggcgctgtt gatggaacag
     9721 gctatcgcca acgaaggcct gttttatcgc ttactctacc tgcagcgcag tctcaccgcc
     9781 gccagcagtc ttgatgatat gctgatgcgc tttcaccgct gggcgcgtga tctcggcctg
     9841 gcaggtgcga gtctgcgcct gtttccggat cgctggcgct taggtgcgcc gtcgaaccac
     9901 actcatctgg cattaagccg tcagtctttc gaaccgctgc gtattcagcg tctggggcag
     9961 gagcagcatt atctggggcc gcttaacgga ccggagctgc tggtggtact accggaagcg
    10021 aaagcggtgg gatcggtggc gatgtcgatg ctgggaagcg atgctgattt aggcgtggtg
    10081 ctgttcacca gccgcgatgc cagtcactat caacaagggc agggaacgca gttacttcat
    10141 gaaattgcgc tgatgttgcc ggagcttctg gagcgttgga ttgaacgcgt atgaccgatt
    10201 tacacaccga tgtagaacgc tacctacgtt atctgagcgt ggagcggcag cttagtccga
    10261 taactctgct taactaccag cgacagcttg aggcgatcat caattttgcc agcgaaaacg
    10321 gcctgcaaaa ctggcagcaa tgcgatgcag cgatggtgcg caattttgct gtacgcagtc
    10381 gccgtaaagg gctgggcgcg gcaagcctcg cattacgcct ctctgcgcta cgtagctttt
    10441 ttgactggct ggtcagccag aacgaactca aagctaaccc ggcgaaaggt gtttcggcac
    10501 cgaaaacgcc acgtcatctg ccgaaaaata tcgacgtcga tgatataaat cgcctgttgg
    10561 atattgatat caacgatccc ctcgctgtgc gcgaccgtgc aatgctggaa gtgatgtacg
    10621 gcgcgggcct gcgtctttcc gagctggtgg ggctggacat caaacacctc gacctggagt
    10681 ccggcgaagt gtgggtgatg gggaaaggca gcaaagagcg ccgcctgccg attggtcgta
    10741 acgctttgtc atggattgag cactggcttg atttgcgcga cctgtttggt agcgaagacg
    10801 acgcgctttt tctgtcgaaa ctgggcaagc gtatctccgc gcgtaatgtg cagaaacgct
    10861 ttgccgaatg gggcataaaa caagggctga ataatcacgt tcacccgcat aaattacgtc
    10921 actcgttcgc tacgcatatg ctggagtcga gcggcgatct tcgtggtgtg caggagctgc
    10981 tgggtcatgc caacctctcc accacgcaaa tctatactca tcttgatttt caacaccttg
    11041 cctcggtgta cgatgcggcg catccacgcg ccaaacgggg gaaataatgc gtttttaccg
    11101 gcctttgggg cgcatctcgg cgctcacctt tgacctggat gatacccttt acgataaccg
    11161 tccggtgatt ttgcgcaccg agcaggaggc gcttaccttt gtgcaaaatt accatccggc
    11221 gctgcgcagc ttccagaatg aagatctgca acgcctgcgc caggcggtac gggaagcgga
    11281 acccgagatt tatcacgacg tgacgcgctg gcgttttcgc tcaatagaac aagcgatgct
    11341 tgacgccgga ttgagcgcgg aagaagccag tgcaggcgca cacgcggcaa tgatcaactt
    11401 tgctaaatgg cgcagcagga tcgacgtccc gcagcaaacc cacgacacct taaaacagct
    11461 ggcgaagaaa tggccgctgg tggcgatcac caacggtaac gctcagccgg aactgtttgg
    11521 tttgggggat tattttgagt ttgtgctgcg cgctggcccg cacgggcgct cgaaaccgtt
    11581 cagcgatatg tacttcctgg cggcggaaaa actcaacgtg ccgatcggcg agatcttaca
    11641 tgtaggtgac gatctcacca ctgacgtggg tggggcaatt cgcagcggaa tgcaggcctg
    11701 ttggatcaga ccggaaaatg gcgatctgat gcaaacctgg gatagccgtt tactcccgca
    11761 tctggaaatt tcccggttgg catctctgac ctcgctgata taatcagcaa atctgtatat
    11821 atacccagct ttttggcgga gggcgttgcg cttctccgcc caacctattt ttacgcggcg
    11881 gtgccaatgg acgtttctta cctgctcgac agccttaatg acaaacagcg cgaagcggtg
    11941 gccgcgccgc gcagcaacct tctggtgctg gcgggcgcgg gcagtggtaa gacgcgcgta
    12001 ctggtgcatc gtatcgcctg gctgatgagc gtggaaaact gctcgccata ctcgattatg
    12061 gctgtgacgt ttaccaacaa agcggcggcg gagatgcgtc atcgtatcgg gcaactgatg
    12121 ggcaccagcc agggcggcat gtgggtcggc accttccacg ggctggcgca ccgtttgctg
    12181 cgtgcgcacc atatggacgc caatctgccg caggatttcc agatcctcga tagcgaagac
    12241 cagctacgcc tgcttaagcg tctgatcaaa gccatgaacc tcgacgagaa gcagtggccg
    12301 ccgcgccagg caatgtggta catcaacagc cagaaagatg aaggcctgcg tccgcatcat
    12361 attcaaagct acggtaatcc ggtggagcag acctggcaga aggtgtatca ggcgtatcag
    12421 gaagcgtgcg atcgtgcagg cctggtggac ttcgccgagc tgctgctgcg cgctcacgag
    12481 ttgtggctta acaagccgca tatcctgcaa cactaccgcg aacgttttac caacatcctg
    12541 gtggacgaat tccaggatac caacaacatt cagtacgcgt ggatccgcct gctggcgggc
    12601 gacaccggca aagtgatgat cgtcggtgat gacgaccagt caatctacgg ctggcgcggg
    12661 gcgcaggtgg agaatattca gcgtttcctc aatgatttcc ccggtgccga aactatccgt
    12721 ctggagcaga actatcgttc taccagtaac attctgagcg ccgcgaacgc cctgattgaa
    12781 aacaacaacg ggcgtctggg taaaaaactg tggaccgatg gcgcggacgg tgagcctatt
    12841 tccctctatt gcgcttttaa cgaactcgat gaagcgcgtt ttgtcgttaa ccgcatcaaa
    12901 acctggcagg acaacggtgg ggcgctcgcc gagtgcgcca ttctctaccg cagcaacgcc
    12961 cagtcgcggg tgctcgaaga ggcgttattg caggccagta tgccgtaccg tatttacggc
    13021 gggatgcgct tcttcgaacg ccaggaaatc aaagatgcgc tctcgtatct gcgcctgatt
    13081 gccaaccgca acgacgacgc ggcctttgag cgtgtggtga atacgccaac gcggggtatt
    13141 ggtgaccgga cgctggacgt ggtacgtcag acatcgcgcg atcgccagtt aacactctgg
    13201 caggcatgtc gtgaactgtt gcaggaaaaa gccctcgccg gacgtgctgc cagcgcctta
    13261 cagcggttta tggaactgat cgacgcctta gcgcaggaaa ctgccgatat gccgctgcat
    13321 gtacagactg accgggtaat taaagactcc ggcctgcgta ccatgtacga gcaggagaag
    13381 ggcgaaaaag gtcagacgcg tatcgaaaac ttagaggaac tagtgacggc aacgcgccaa
    13441 ttcagctaca acgaagaaga cgaagattta atgccgctgc aggcgttcct ctcccatgcg
    13501 gcgctggaag cgggcgaggg gcaggcggat acctggcagg atgcagtgca gttgatgacg
    13561 ctgcactcgg cgaaaggcct ggagttcccg caggtgttta tcgtcggtat ggaagagggc
    13621 atgttcccaa gccagatgtc gctggatgaa ggcggacgtc tggaagaaga acgccgtctg
    13681 gcctacgttg gtgtaacccg cgcgatgcag aaactgacgc tgacctacgc ggaaactcgc
    13741 cgtctgtatg gcaaagaggt ttaccatcgc ccgtcgcgct ttatcggtga gttgccggaa
    13801 gagtgtgtgg aagaggtgcg cctgcgcgcc acggtaagcc gcccggtcag ccatcagcgt
    13861 atgggtacgc cgatggtcga gaacgacagc ggctacaagc tcggccagcg tgtacgccac
    13921 gctaagtttg gtgaaggcac tatcgtcaat atggaaggca gcggcgagca tagccgtttg
    13981 caggtggcat tccagggcca gggtattaaa tggctggtgg cggcatacgc ccggctggag
    14041 acggtataac gttgcgggat gcggtgcgcc acaccgcatc cggcatcagc accttactta
    14101 tcacatttct tcacatcaat attccagtca gcaccagacg caacctgatg tttctgcgcg
    14161 aaattatcca tattcagcgc cacggaaaca ttcaataagc tgttcagata caccatcggc
    14221 tgtccttccg gcacatcgcc gaagcttgcg acatacggca ttttccctgc gtatttttgc
    14281 cgggaacctt cggaaatggt cacacacaac gtgtcgttga gtttgatccc cgcctgattc
    14341 agcaactcgt cgctgatatt gctccagacg ttgccgtact gaatatcgag aatcgggata
    14401 ttgcccttca cttcaccttt tgttgctttt gctttctgat aagaaagttc gacaaccttt
    14461 gcaggaagct caggccccac ctgctcgaac gtgatcgccc cggaagccag acgtgcaccg
    14521 gtgtaggcat acacgtcacg accgtggaag gtatatgact tttcagaacc tttcaggcgg
    14581 ttagcttttt cgtcgatttc ccggacgctc tcaataccca gcgattccgc caccaaagtg
    14641 agcgtaccgt tatccggtga aacgaagtat tgaccgtttt tggttttcag cactatcgat
    14701 ttacggtcag tgcctacgcc cggatcgacc acactgacaa ataccgaacc ttgcggccag
    14761 taactggcgg tctgatacag gcggtaagcg ccttcccaga tgttatacgg tggaatttcg
    14821 tgtgttaaat caaatatttt aagattatgg tcaacaccga atgcgacccc tttcatcgcc
    14881 gagacggcac catctttcag gctaaaatca gtttgtaaaa tcaaagcgtt atccgcataa
    14941 gcggatgatg atcccagaca cgttaataaa aataacgttg cgagcgtttt tttcattttt
    15001 gtttcccttc atttatctga tgattttcca gctgtgtagc cagccccagt ttgtctggcc
    15061 tcctcgagtg tacaatgctt aacctttccg ctcgacggaa atcatggtga caaaaggata
    15121 gcgttgccac gggattgccc cgcctttcat atacatatgt gaaatcgtgc catcaagata
    15181 aagcagctgc tcaacgttca gtttcgcttt ggcataacag gcaaaatcat aaaaatttgt
    15241 cgcctgctgg ctcaacaaaa acacggcatt cccatgttta ttaattccaa caccgttacg
    15301 aattttacgt gaggcgacgt tgggatgaat acgcggatta attacactgt tttccatcaa
    15361 cattggccct gactgcaccg caaactgaat ctctttactg gttttgaagg catccagacg
    15421 aacgatgccg actttatctc ccgcgacata aaacacgccg ccaggacgga taaagaaatt
    15481 cccttcacct gaagcgagat ttaacgccac cttctgctga ccgttttcga tgtacaaacc
    15541 gagcggcgca tagctttcat catagatgcc gccgttcatc gccatctgca cctgaccctg
    15601 actattcata tccgccagca gagcatgtaa agttccccac gcttcgccat tggctttttg
    15661 ccagtacatt ttcacccgct ctgtttgagg attaacggta tacgcctgta cggtcagcgt
    15721 tggatctgag agtgcgcaat catcagcggc aacagcaaac aagggaagta aggtgagggc
    15781 gagaaaaatc cgtttgagat tcaaggtgat cattccttta ccaatgagta gccgatgcgc
    15841 cattataggt cctggatgtg ggattttttt atcctgttag cgaccttgac gagtaccaaa
    15901 aagcgcgaag ttcaactatt gttctgtggt gttctgttgc gtgttgacgg caaaattttg
    15961 ctggcgtaac atgcgcgcac gatcactcta agaggacatt cgccttggac acacccagta
    16021 gatactggct cactatcctg tcatccagga tcaactccta aggctatccc tttttgctga
    16081 tagccttagc ggttgtcagc gacctcaatt tttcccgtcg cgctgagtca ggctgtttaa
    16141 tggtctgaaa cccaatttgt ttctgtgtgc ccaccgaact gtccgatatt ttaagcattg
    16201 ggagtcccgg tcatgctgag cgcatttcaa ctggaaaata accgactgac ccggctggaa
    16261 gtcgaagagt cacaacccct tgtaaatgca gtatggattg atcttgtcga accggacgac
    16321 gacgagcgac tgcgcgtaca atctgaactt ggccagagcc tggcaacccg cccggaactg
    16381 gaagacatcg aagcatcggc acgtttcttt gaagacgatg acggcctgca tattcactcc
    16441 ttcttcttct ttgaagatgc ggaagatcac gccggtaact ccactgtggc atttaccatc
    16501 cgtgatggtc gtctgtttac tctgcgtgag cgtgaactgc ccgcttttcg tctgtatcgt
    16561 atgcgtgccc gtagccagtc gatggtagac ggtaacgcct acgagttgct gctggatctg
    16621 ttcgaaacca aaatcgaaca gttggcagat gaaattgaaa atatctatag cgacctggag
    16681 caattgagcc gggtgattat ggaagggcat cagggcgatg agtacgacga ggcgctctcc
    16741 actctggcgg aactggaaga tatcggctgg aaagtgcgcc tgtgtctgat ggatacccag
    16801 cgcgcgctca acttcctggt acgtaaagca cgcttaccgg gtgggcaact ggagcaggcg
    16861 cgtgaaatcc tgcgagatat cgaatccctg ctgccgcata acgaatccct gttccagaag
    16921 gtgaacttcc tgatgcaggc ggcaatgggt tttatcaaca tcgagcagaa ccgcatcatc
    16981 aaaattttct cggtggtatc cgtcgtgttc ctgccgccaa cgctggttgc ttccagctac
    17041 gggatgaact ttgagtttat gccagaactg aagtggagtt tcggctaccc aggcgcgatt
    17101 atctttatga tcctcgcggg cctggcaccg tatctgtact ttaagcggaa gaactggttg
    17161 taatatgagt gccggataaa gctatttttt tatccggctt atttctaaaa attgtctttt
    17221 ttaaagttat gcggattgaa aacttgctct attttatttt cttgtgttaa ttgatactca
    17281 gtaaaatcgt taataccaat atgagtgtta cgataagggt attcattaat aaccattaag
    17341 taatttttcg tgatgcaatt atcattgctc ttaattgtcg ggaaacagta gagatcttca
    17401 tacggccctt cgatatattt tttatagtca gtgaagccat taccgatatc aatgacatac
    17461 caggtatgcc aactaccttc tggtagggga tgttgatttt taatttcatt ctgatgcttc
    17521 agataccaat ttattttatc gctgtcagtc catggcagat gatcaacgac cagaaagact
    17581 gaattgccag catcagagta tgtatacaaa atctgtacag gacgcaatgt ccataaatta
    17641 tagatgcagc atagaagaaa taaacataat ataatttttt ggagtttgct tttcatagat
    17701 aattttcgat tctttcgata gtgttaaaat tggtgaaaaa aggtttgaaa gcaaagtctt
    17761 tatgccgttg taaaaagaac catatgcgaa aaaatctata tttgctgtaa gtcttgtttt
    17821 tgatatcagt tacatcaaga ccgaagtggt cctgtgctac
//
LOCUS       DAZKIP010000091        17574 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZKIP010000091 DAZKIP010000000
VERSION     DAZKIP010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17574
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.091"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..613)
                     /gene="espY1"
                     /locus_tag="ACHOUK_003322"
     CDS             complement(<1..613)
                     /gene="espY1"
                     /locus_tag="ACHOUK_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspY1"
                     /protein_id="HFP9898710.1"
                     /translation="MKVSVPGMPATLLNMSNNDIYKMVSGDKMDMKMNIFQRLWETLR
                     HLFWSDKQTEAYKLLFNFVNKKAGNINASKYFTGAVNENEKEKFIHSLELFNELKTCA
                     KNPDEMVAKGNMSWVAQTFGDIELSVTFFIENKEICTQTLQLHKGPGNLGVDLREAYL
                     PGVDMRDCYLGLKTMKGHNKVLYLEPGWNANLDGATLDGATLDG"
     gene            complement(908..1723)
                     /gene="djlA"
                     /locus_tag="ACHOUK_003323"
     CDS             complement(908..1723)
                     /gene="djlA"
                     /locus_tag="ACHOUK_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFP9898711.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            1978..4332
                     /gene="lptD"
                     /locus_tag="ACHOUK_003324"
     CDS             1978..4332
                     /gene="lptD"
                     /locus_tag="ACHOUK_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFP9898712.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINANHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVSIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            4385..5671
                     /gene="surA"
                     /locus_tag="ACHOUK_003325"
     CDS             4385..5671
                     /gene="surA"
                     /locus_tag="ACHOUK_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFP9898713.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            5671..6660
                     /gene="pdxA"
                     /locus_tag="ACHOUK_003326"
     CDS             5671..6660
                     /gene="pdxA"
                     /locus_tag="ACHOUK_003326"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFP9898714.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            6657..7478
                     /gene="rsmA"
                     /locus_tag="ACHOUK_003327"
     CDS             6657..7478
                     /gene="rsmA"
                     /locus_tag="ACHOUK_003327"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFP9898715.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPFQES"
     gene            7481..7858
                     /gene="apaG"
                     /locus_tag="ACHOUK_003328"
     CDS             7481..7858
                     /gene="apaG"
                     /locus_tag="ACHOUK_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFP9898716.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            7865..8713
                     /gene="apaH"
                     /locus_tag="ACHOUK_003329"
     CDS             7865..8713
                     /gene="apaH"
                     /locus_tag="ACHOUK_003329"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFP9898717.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     GEAVAS"
     gene            complement(8710..9024)
                     /locus_tag="ACHOUK_003330"
     CDS             complement(8710..9024)
                     /locus_tag="ACHOUK_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HFP9898718.1"
                     /translation="MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFS
                     RIRPPERLNPILLLVDGKEYVLMTHETATVPVNALGTKFCDASAHRTLIKGALDFMLD
                     GI"
     gene            complement(9027..9260)
                     /locus_tag="ACHOUK_003331"
     CDS             complement(9027..9260)
                     /locus_tag="ACHOUK_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HFP9898719.1"
                     /translation="MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKH
                     AATRWREENAEAIAALNQLADETGCFSDEYRSF"
     gene            complement(9346..9825)
                     /gene="folA"
                     /locus_tag="ACHOUK_003332"
     CDS             complement(9346..9825)
                     /gene="folA"
                     /locus_tag="ACHOUK_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFP9898720.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTRESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(10017..11879)
                     /gene="kefC"
                     /locus_tag="ACHOUK_003333"
     CDS             complement(10017..11879)
                     /gene="kefC"
                     /locus_tag="ACHOUK_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFP9898721.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYICLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(11872..12402)
                     /gene="kefF"
                     /locus_tag="ACHOUK_003334"
     CDS             complement(11872..12402)
                     /gene="kefF"
                     /locus_tag="ACHOUK_003334"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFP9898722.1"
                     /translation="MILIIYAHPYPHHSHANKRMFEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(12510..13841)
                     /locus_tag="ACHOUK_003335"
     CDS             complement(12510..13841)
                     /locus_tag="ACHOUK_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9898723.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYILVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(13899..14186)
                     /gene="fixX"
                     /locus_tag="ACHOUK_003336"
     CDS             complement(13899..14186)
                     /gene="fixX"
                     /locus_tag="ACHOUK_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFP9898724.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(14183..15469)
                     /locus_tag="ACHOUK_003337"
     CDS             complement(14183..15469)
                     /locus_tag="ACHOUK_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9898725.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTNVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(15520..16461)
                     /locus_tag="ACHOUK_003338"
     CDS             complement(15520..16461)
                     /locus_tag="ACHOUK_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFP9898726.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINAFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYASVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(16476..17246)
                     /locus_tag="ACHOUK_003339"
     CDS             complement(16476..17246)
                     /locus_tag="ACHOUK_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFP9898727.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT         4352 a         4860 c         4338 g         4024 t
ORIGIN      
        1 caccgtccag ggtagcaccg tccagggtag caccgtccag gttagcattc cacccaggtt
       61 ccaagtaaag gactttattg tgacctttca ttgttttgag accaaggtaa caatccctca
      121 tgtcaacgcc gggaaggtaa gcctctctaa gatcaacgcc caagttacct gggcctttat
      181 gcagctgcaa cgtctgagta catatctcct tattttcaat aaaaaaagtg acacttaact
      241 cgatatcacc gaaggtctgt gcgacccagc tcatattgcc ctttgcgacc atctcatccg
      301 gattttttgc gcatgttttg agttcattga ataattccag actatggata aacttttctt
      361 tctcattctc gttgacagcc ccagtaaagt attttgaggc gttgatgttg ccagcctttt
      421 tattcacgaa attgaacaga agtttataag cctcagtctg tttatctgac cagaacagat
      481 gacgtaacgt ctcccacaag cgttgaaaga tattcatctt catgtccatc ttgtccccgc
      541 tcaccatctt ataaatatcg ttattgctca tatttaaaag tgtagccggc atgcctggaa
      601 ctgatacttt catggtaata gcctcgcatt gagttaaaaa ctcatgatgc gcgctctgaa
      661 taaatatact ttcaaaaatc gctcattcag atatgtgttt tttattttta agacgtaaaa
      721 atataattcc gctcgccgta aagctctcaa ccttaagcag ggtcaacgcg ataaaaccaa
      781 cagaaaccct cccgtaaacc gtatattgag cctgtccacc attctgagta aacgcctttt
      841 ctcaaagaag tgaccgccag ggcggccacc ttttatcgtg ggcagttact cagcatcatt
      901 tacagggtca tttaaaccct ttctgctgct taatcagctc ataggcctgc tgaatttcct
      961 gcgctttctg cttcgccatc tccatcatct caggcggcaa acctttcgcc accagcttat
     1021 cgggatggtg ttcactcatc agcttacggt aggcacgttt gatggtggtc gcatcatccg
     1081 tcggcttcac gcccagcaca ttacaggcat cttccagcgt tgggccacgc tgcgcttgct
     1141 gccagttacc accgccagat tgctgctgat aaccgccgcc aaactgtgca ccgccctgca
     1201 tcatgcgcaa aaactggtca aactgggcgc gggagatccc taattcttca gcaatgacat
     1261 acagcaccgc ccgttcattc gggtgcagtg aaccatcagc aaacgccgcc tgaatctgga
     1321 tctccagaaa catacgaatt aagtcaaaac gaccaaagca gacactgcga aactggcgca
     1381 tcttttcgcg cagcgggtaa ttgtctgatt ttcccacccg gaacgcattt tgcgccgcag
     1441 tacgggaagc gccatgaaga ttcattcggt ccatcaactg gctggcgata tgaatatcag
     1501 cctccgtgac gcgacctttg gatttggtta aatgccccat cacttcaaaa gtggtggcaa
     1561 aaaacagcgc ctgacgctca cgctggttgg cgaaccacgc cattttacgg ctacgggctt
     1621 tatcaaacat atggccaatt aacaggccta acactacgcc ccaaaagccg ccgcccatca
     1681 gtaaggccac ggccacgcca atgatttttc cccaatactg catatattcc ccaaatcgac
     1741 acacggatat cagggctatc tcccacaata taaaggtgct tttaccgttt tccggctgcg
     1801 gtcaattgtg ggacatcgcc tataatttgc attatcatac ctgtcattca atgccgtgcc
     1861 taacaccact gacgctattc ggacaggatt aacactagcg tagagatgac gagtacgtta
     1921 gtctctgacc gtttgtcacg cgcaacgtta ccgatgatgg aacaataaaa tcaacgtatg
     1981 aaaaaacgta tccccactct cctggccacc atgattgcca ccgcccttta tagtcaacag
     2041 ggactggcag ccgacctcgc ctcacagtgc atgttgggcg tgccaagcta tgaccgtcct
     2101 ctggtacagg gcgataccaa tgacttaccc gtgactatca atgctaacca cgcgaaaggg
     2161 gactacccgg atgacgccgt gtttactggc agcgtggata tcatgcaggg taacagccgt
     2221 ctgcaggccg acgaagtgca gctccatcaa aaagaggcac caggacaacc ggagccggta
     2281 cgtaccgttg atgcgctcgg taatgtccat tacgacgata accaggtgat cctcaaaggg
     2341 ccgaaaggct gggcgaatct gaacaccaaa gataccaacg tctgggaagg tgattaccag
     2401 atggtgggtc gccagggtcg cggtaaagcg gacctgatga aacaacgtgg cgaaaaccgc
     2461 tataccattc tggataacgg tagctttacc tcctgtctgc cgggttctga cacctggagc
     2521 gtggtaggta gcgaaattat tcatgaccgc gaagaacaag ttgcggagat ctggaacgcc
     2581 cgctttaagg tgggtccggt atcgatcttt tatagcccct atttgcagtt gccggtgggt
     2641 gacaaacgtc gctctggttt cttgatcccg aacgccaagt acaccaccac caactacttt
     2701 gagttctacc tgccatatta ctggaacatc gcgccaaata tggatgccac catcacgccg
     2761 cattatatgc atcgtcgtgg caacatcatg tgggagaacg aattccgcta cctctcccag
     2821 gcgggcgctg gcttgatgga actggactat ctgccttcag ataaagtcta tgaagatgaa
     2881 cacccgaacg atgacagttc acgtcgttgg ttattctact ggaaccactc cggggtcatg
     2941 gatcaggtgt ggcgtttcaa cgtcgactac accaaggtca gcgatcctag ctacttcaat
     3001 gatttcgata acaagtacgg ttccagtact gacggctacg caacgcaaaa attcagcgtt
     3061 ggctatgcgg tgcaaaactt caatgccacc gtttcaacca agcagttcca ggttttcagc
     3121 gaacagaaca ccagtagcta ctcggcagag ccgcagttag acgttaatta ctaccagaat
     3181 gatgttggtc cgtttgatac gcgtatttac ggccaggcag tgcactttgt taacaccaga
     3241 gacgacatgc ctgaagcaac ccgtgttcac ctggaaccga ccatcaattt gccgctctct
     3301 aataactggg gcagcatcaa taccgaagcg aagttgctgg caacccatta tcagcaaacc
     3361 aatcttgact ggtataactc cagaaacacg accaagctgg acgaatccgt taaccgcgta
     3421 atgccgcaat tcaaagttga cggcaaaatg gtctttgaac gcgatatgga aatgctggct
     3481 ccgggttata cccaaacgct ggaaccgcgc gcgcagtatt tgtacgtgcc gtatcgcgat
     3541 cagagcgaca tctataacta cgactcgtct ctgctgcaat ctgactactc tggcctgttc
     3601 cgggaccgga cttacggcgg tcttgaccgt attgcctccg ctaaccaggt gacgaccggt
     3661 gtcacatctc gcatatatga tgatgctgcc gttgaacgtt ttaatatttc cgttggtcaa
     3721 atctactatt tcacggagtc tcgcactggc gatgacaaca taacatggga gaatgacgac
     3781 aaaacgggtt cactggtgtg ggcaggcgat acttactggc gtatctccga gcgttgggga
     3841 ttgcgtggcg ggattcagta cgatacacgt ctggataacg tagcgaccag taactccagc
     3901 attgaatacc gtcgggatga agaccgtctg gtacagctga attaccgtta cgccagcccg
     3961 gaatatattc aggctacgct gcctaagtac tattccactg ctgagcaata taagaatggt
     4021 atttcgcagg taggtgctgt cgccagctgg ccaattgccg atcgttggtc cattgttggg
     4081 gcctactact acgacaccaa tgcgaacaag caagccgact ctatgttagg tgtgcaatac
     4141 agctcctgct gctatgcaat tcgcgtcggt tacgagcgga agctgaacgg ttgggataac
     4201 gataaacaac atgcggtata tgacaacgca atcggcttta acatcgaact tcgcggcctg
     4261 agctccaact acggtctggg tacgcaagag atgctgcgtt cgaacattct gccgtatcaa
     4321 aacactttgt gatttgttga tttaccacgt aatccgcagt gcggttaatt gaaatggaaa
     4381 aagtatgaag aactggaaaa cgctgcttct cggtatcgcc atgatcgcga ataccagttt
     4441 cgctgccccc caggtagtcg ataaagtcgc agccgtcgtc aataacggcg tcgtgctgga
     4501 aagcgacgtt gatggattaa tgcagtcggt aaaactgaac gctgctcagg caaggcagca
     4561 acttcctgat gacgcgacgc tgcgccacca aatcatggaa cgtttgatca tggatcaaat
     4621 catcctgcag atggggcaga aaatgggagt gaaaatctcc gatgagcagc tggatcaggc
     4681 gattgctaac atcgcgaaac agaacaacat gacgctggat cagatgcgca gccgtctggc
     4741 ttacgatggt ctgaactaca acacctatcg taaccagatc cgcaaagaga tgattatctc
     4801 tgaagtgcgt aacaacgagg tgcgtcgtcg catcaccatc ctgccgcagg aagtcgaatc
     4861 cctggcgcag caggtgggta accaaaacga cgccagcact gagctgaacc tgagccacat
     4921 cctgatcccg ctgccggaaa acccgacctc tgatcaggtg aacgaagcgg aaagccaggc
     4981 gcgcgccatt gtcgatcagg cgcgtaacgg cgctgatttc ggtaagctgg cgattgctca
     5041 ttctgccgac cagcaggcgc tgaacggcgg ccagatgggc tggggccgta ttcaggagtt
     5101 gcccgggatc ttcgcccagg cattaagcac cgcgaagaaa ggcgacattg ttggcccgat
     5161 tcgttccggc gttggcttcc atatactgaa agttaacgac ctgcgcggcg aaagcaaaaa
     5221 tatctcggtg accgaagttc atgctcgcca tattctgctg aaaccgtcgc cgatcatgac
     5281 tgacgaacag gctcgtgtga aactggaaca gattgctgct gatatcaaga gtggtaaaac
     5341 gacttttgct gccgcagcga aagagttctc tcaggatcca ggctctgcta accagggcgg
     5401 tgatctcggc tgggctacac cagatatttt cgatccggcc ttccgtgacg ccctgacccg
     5461 cctgaacaaa ggtcaaatga gcgcaccggt tcactcttca ttcggctggc atttaatcga
     5521 actgctggat acccgtaatg tcgataaaac cgacgctgcg cagaaagatc gtgcataccg
     5581 catgctgatg aaccgtaagt tctcggaaga agcagcaagc tggatgcagg aacaacgtgc
     5641 cagcgcctac gttaaaatcc tgagcaacta atggttaaaa cccaacgtgt tgtgatcact
     5701 cccggcgagc ccgccgggat tggcccggac ttagttgtcc agcttgcaca gcgtgagtgg
     5761 ccggtcgaac tggttgtttg tgccgatgcc actctcctta ccgaccgggc agcgatgctc
     5821 ggtttgccgc tcaccctccg cccttattcc cccaactccc ctgcacaacc gcaaactacg
     5881 ggcacattaa cgctacttcc tgtcgcgcta cgtgaatctg tcactgcggg gcagttagcg
     5941 attgaaaatg ggcattacgt ggtggagacg ctggcgcgag cgtgtgatgg ctgtctgaac
     6001 ggtgaatttg ctgcgctgat cacaggcccc gtgcataaag gcgtcattaa cgacgcaggc
     6061 attccgttta ccggtcatac cgagtttttc gaagagcgtt cgcaggcgaa aaaagtggtg
     6121 atgatgctgg cgaccgaaga acttcgcgtg gcgctggcaa cgacgcattt accgctgcgc
     6181 gatatcgcag atgctatcac ccctgcactt ttgcacgaag tgattgctat tttgcatcac
     6241 gatttgcgta caaaatttgg tattgccgaa ccgcgcattc tggtctgcgg gctgaatccg
     6301 cacgcgggcg aaggcggtca tatgggtacg gaagagatag acaccattat tcctgtgctc
     6361 gacgagctgc gggcgcaggg gatgaaactc aacgggccgc tgcctgccga taccctgttt
     6421 cagccgaaat atctcgataa cgccgacgcc gtgctggcga tgtaccacga tcagggtctt
     6481 cccgtgctaa aataccaggg cttcgggcgc ggtgtgaaca ttacgctggg cctgcccttt
     6541 attcgcacat cagtggacca cggcaccgca cttgaactgg cgggacgtgg cgaagccgat
     6601 gtcggcagtt ttattacggc gcttaatctc gccatcaaaa tgattgttaa cacccaatga
     6661 ataatcgagt ccaccagggc cacttagccc gtaaacgctt cgggcaaaac tttctcaacg
     6721 atcagttcgt gatcgacagc attgtttctg ccattaaccc gcaaaagggc caggcaatgg
     6781 tcgaaatcgg ccccggtctg gcggcattga ccgagccggt cggcgaacgt ctggaccagc
     6841 tgacggtcat cgaacttgac cgcgatctgg cggcacgtct gcaaacgcat ccattcttag
     6901 gcccgaaact gacgatttat cagcaggatg cgatgacctt taactttggc gaactggccg
     6961 agaaaatggg tcagccgctg cgtgttttcg gcaacctgcc ttataacatc tccacgccgt
     7021 tgatgttcca tctgtttagc tatactgatg ccattgccga catgcacttt atgttgcaaa
     7081 aagaggtggt gaatcgtctg gttgcaggac cgaacagcaa agcgtatggt cgattaagcg
     7141 tcatggcgca atactattgc aacgtgatcc cggtactgga agttccgccg tcagccttta
     7201 caccaccacc caaagtggat tccgccgtcg tgcgcctggt tcctcatgca acgatgcctc
     7261 acccggttaa agatgttcgt gtgttgagcc gcatcaccac cgaagccttt aaccagcgtc
     7321 gtaaaaccat tcgtaacagc ctcggcaacc tgtttagcgt cgaggtgtta acgggaatgg
     7381 ggatcgaccc ggcgatgcga gcggaaaata tctctgtcgc gcaatattgc cagatggcga
     7441 actatctggc ggagaacgcg ccttttcagg agagttaacg atgatcaatt cgccccgagt
     7501 gtgtattcag gttcaaagcg tctacattga ggcacaatct tcacctgata atgaacgtta
     7561 cgtttttgct tataccgtaa ccatacgcaa tctggggcga gcgccagtgc agttgttggg
     7621 gcgttactgg ctgatcacca atggcaatgg ccgtgaaacc gaagtccagg gcgaaggagt
     7681 ggttggcgtc cagccactta tcgcgcctgg cgaagagtac cagtacacca gcggtgcaat
     7741 cattgaaacc ccgctgggca ccatgcaggg tcactacgaa atgatcgatg aaaatggcgt
     7801 ccctttcagc atcgacattc ccgtattccg actcgccgtt cccacactca ttcattaaaa
     7861 gaatatggcg acatacctta ttggcgacgt tcatggttgt tacgatgaac tgatcgcatt
     7921 gctgcataaa gtagaattta cccctgggaa agataccctc tggctgacgg gcgatctggt
     7981 cgcgcgcggc ccgggttcgc tggatgttct gcgctatgtg aaatccttag gcgacagcgt
     8041 acgtctggtg ctgggcaatc acgatctgca tctgctggca gtatttgccg ggatcagccg
     8101 caataaaccg aaagatcgcc tgacaccgct gctggaagcg ccggatgccg acgagctgct
     8161 taactggctg cgtcgccagc ctctgctgca aatcgacgaa gagaaaaagc tggtgatggc
     8221 ccacgcaggg atcacgccgc agtgggatct gcagaccgcc aaagagtgcg cgcgcgatgt
     8281 agaagcggtg ctgtcgagtg actcctatcc cttctttctc gacgctatgt acggcgatat
     8341 gccaaataac tggtcaccgg aattgcgggg gctgggaaga ctgcggttta tcaccaacgc
     8401 ctttacccgt atgcgttttt gcttcccgaa cggtcaactg gatatgtaca gcaaagaatc
     8461 gccggaagag gcccctgccc cactgaaacc gtggtttgcg attcctggac ctgtcgctga
     8521 agaatacagc atcgcctttg gtcactgggc atcgctggaa ggcaaaggta cgccggaagg
     8581 tatttacgcg ctggataccg gctgctgctg gggtgggtca ttaacctgcc tgcgctggga
     8641 agataaacag tattttgtcc agccgtcgaa ccggcataag gatttgggtg agggagaggc
     8701 ggtcgcgtct taaatcccgt cgagcataaa atctaatgct cctttaatca acgttcggtg
     8761 cgcgctggca tcgcaaaatt tcgttcccag cgcgttaact ggaacagttg cagtctcatg
     8821 cgtcatgagc acatattctt tgccgtctac cagtaaaagg attgggttaa gccgttctgg
     8881 tgggcgaata cggctaaatc gttcaatagg tgttaatgga ataacaatac ggcgattaat
     8941 cacaccaata atgtcgctgg taacatcgat aacaaaggga aacgcggcgt tcctgctgcg
     9001 actgcggtat accgtaaatt gcatcgctag aagctccggt actcatcaga gaaacatccg
     9061 gtttcatcag ccaattgatt taacgcagcg atagcttcag cgttctcttc acgccaacgt
     9121 gttgctgcat gttttttaag ttcagcattg agcgcaaccg taagggtggc gctcatattt
     9181 aagcctgcat cacgagcgcg attgactaac tcacggtcga cggtgacggt cacactttgt
     9241 gtggtacgtt ttgcagtcat gatatcgtgc tcctgtacaa tttatacgtc tagtatacac
     9301 ggatatctgc acgtatcagt gatgccggaa ttctaatata caaaattacc gccgctccag
     9361 aatctcaaaa caatagctgt gagagttctg cgcatcagcg tcgtggaatt cgctgaatac
     9421 cgattcccag tcatccggct cgtaatccgg aaaatgggtg tcgccttcca cttctgcgtc
     9481 gatatgcgtc aggtacagtt tttgcgcttt cggcaggaac tgctcataaa cgcgaccgcc
     9541 gccaatcacc atgatttctg gtacgtcacc acacgccgcg atggcttcat ccaccgactt
     9601 cacccacgtt acgcgatcgt ccgtacccgg ttgactgctg aggataatat ttttgcgtcc
     9661 tggcaacgga cgaccgatgg attccctggt atggcggccc ataattacgg gtttatttaa
     9721 ggtgttgcgt ttaaaccagg cgagatcggc aggcaggttc cacggcatgg cgttttccat
     9781 gccgataacg cgatctaccg ctaacgccgc aatcagactg atcattgaga tttcccgata
     9841 aaaaaaattg tcgccactat acgtaaagcg taaaccgtcg tcgactggtg cgaggatgat
     9901 gttgaggaaa attttatatt ctgctggcga gtccacgctc tctccctgga ctcgccgcat
     9961 tacaatgaaa caaaaacaaa cagttagctg taaagtgtga tttacgtcac tctttattag
    10021 gatgagggtt tcgtttccgg ttcatccgcc atgttgccgg tatgtttacc ttcttctgtt
    10081 ccctgccagc catgtcgttg aattaatgac agatgttcgc ggtcctcggt gatgatctca
    10141 cttaacatcg cgctggtgcg tttatagacc gccgcgcggg ctttggtgtc attctcaacc
    10201 attgccatct cttccaccat ctgaatatta aagcggcgga acacatcggc acgttctcgc
    10261 gcttcatacg gccccagacc taaactttcc agcgccagac gcccggtttt cagcgcacct
    10321 tcaaaggttt cacgctctgg cttttcaacg cctgcctgac gcaaacaaat gtagtgatcg
    10381 acatcgcggg cgcgggcaat aatctgcaaa tgcgggaaat gttctttcac catctccgtc
    10441 agttgcaggt tggtttgcgg atcgtcaatg gcgttaatca gcacttccgc tttcgccgct
    10501 cccgcagatt ccagtaaatc catccgcgtg gcatcgccat aaaacacttt cataccaaat
    10561 ttacgcagcg tttcgatatg gtccggatcg tgatcgagca ccaccatttt caccccgctg
    10621 gagagcagta aacgtccggt gatctgcccg aaacgaccga atccggcaat gattacccgc
    10681 ggctgttctt cgtcgatctc atcggcttca cgcgcctctt cagtagaaga ttgctcaagg
    10741 cgattgagga tcaccagcag aatcggcgtt gccgccatcg acagcgccac cgccagggtc
    10801 agcgatttcg cccactccgg ctccagcaca ttcgccatct gcgccgcgcc aaataccaca
    10861 aaggcaaact cactgccctg ccctaacaac accgcaaacc agcgacgctg tttatttggc
    10921 acctgcaacg gtcgggcaat cagccacagc atggcgattt tgatgatgag gaaaccgagc
    10981 agcaaaatga caatgcgcaa tgggttttca atcagcgtgc caaagtctat cgacatgcca
    11041 acaccgatga aaaacagccc caacagcaaa cctttaaagg gttcgatatc gctttccagc
    11101 gcatgacggt attccgagct tgccagcagt acgcccgcca gaaacgcgcc catcgccatc
    11161 gacaagccga cctcttccag cagcaaacca aagccaaaca caaggaataa cgccacggca
    11221 ctaaacactt cccgcaagcc agagcgggca acaaaacgca gcgccggacg cgtgacatag
    11281 cgccccagca gtaccaccag caccagcgcg cccgccactt ttaacgccga gagagcaaat
    11341 gcgcccatcg tcgtcgaggc actgctcgcc gccagtagcg ggatcatcgc caccagcggg
    11401 attgccgcga tatcctggaa cagcagcacc gcaaaggcac tgcggcccat ttgcgtcacc
    11461 atcagattgc gttcattcat cgcctgcatg gcaatcgccg tagaggagag cgccagcgtc
    11521 atgccgatca attccgcgac ctgccagcgc agcccaagta acatacagaa cagccccagc
    11581 aggccgccgc aaatcaccat ctgcaatgcg ccaccgccaa acactgccgc acgcagcttc
    11641 cacagccttt gtggatcgag ttcgaggccg ataataaaca gcatcagcac cacgccaatc
    11701 tcggcaaagt gcagaataga ttcggcatcg gtcaccagtc gcagccccca cgggccaata
    11761 atgcagccgg cgatcaggta gccaagtacc gatcccagac caagacgtac tgcaatgggt
    11821 acaatcagcg ccgccgaacc gagataaatc agcgcctgaa ccagcgtatg gctatccatg
    11881 atgggcctcc tgccattcca gcagacgttg cttatagtga cgcgcctgcc cttcgagggt
    11941 ttcgtcgtca caaataaagg tgcagtgcat ggcaaacggt ggcagccagt tcagcccgca
    12001 gtagattgcc gtcgcctgta gcggctgcga cagcacatca aagcccggat gcgcaccaat
    12061 ttcaaaatgg ctttccccgc cgccggtcgt caccgcccac agcaaatgtt tgccatgcag
    12121 cgccgtgccg ccgtgaccgt aagcccagcc gtgcgagaag actttatcga tccaaagttt
    12181 aaggagcgga ggaatgctgt accactgcat cgggtgctgc cagacgatca gatcggcgcg
    12241 agacagcgcc tcctgctcgg cggcaatatc gatattgaag tcaggataga gttgataaag
    12301 agagcgaatt tcgacgcctt ccagcgtcct tgcctgttca aacatccgtt tattcgcatg
    12361 ggaatgatgc ggatacggat gcgcataaat tataagaatc ataggtagcc cgcgtgttta
    12421 cgcctttgtt ttcccacaaa gtgtagtcat atattcacgc ggcttatagt caataatatt
    12481 gattcagaaa agagataaat ctgaacaaat taccccattc tctgcccgcg gatcgtcata
    12541 ttgctggtct gcgccagtga catccctcga gtctccgggg caaacgctac ggaaatcaac
    12601 aagccaaaca gcgagatacc cgcccccatt agcatcgtgt tactgatacc gtaattattg
    12661 ataaagatcg gtagtgccca ggtcgaaaca atggtgccaa tacgacttaa ggacataatc
    12721 acgcccacgg cagaggcgcg gatatccgtc gggaagagtt cattaggata gagccactgc
    12781 aaattacccg gcccgccaga gaaaaaggca tacaccgcaa aggccatcac taccagccag
    12841 atccccatat ccgggatcag ccccaaaacc gccagcgcca gcgtcatcat ggcaaagctg
    12901 ccaatcaaca atggacgccg tccggcagtg tttagccaca gcatcggcgg aatacagccg
    12961 agcataaaga acaggctaat caccacgttc cccagtgccg cgtttttgcc aacacccaat
    13021 cccaacaaac caacgatttg cgggccaaag gtgtaaatgg cgaacattgg gatcacctgg
    13081 caggtccaga tggcggcaac aaacagaaca aaaggaaaat ggcggcgatt aaacagatcg
    13141 cgaaaacggg tttgctgcgg ctgctcttca tcgaaagcca ccggttcgcc aaacagtttg
    13201 atcatcatct cttcgcactc ttttactcgc cctttgcgta ataaccagcg gggagattca
    13261 ggcagttcga atcgaccaat caaaatcaac aaacagggga tcgccgcgct acccagcatc
    13321 cagcgccagc cgccttccac atcataaagc cagtagccga ccagatcggc acaggtcgcg
    13381 ccgacatacc acatggcggc gataaagctg atggaaaacg cccgctgacg ggtactggag
    13441 aactcagtga tcatcgaagt ggcgatggga tagtctgcac cgatgacaat gccgataagt
    13501 acccgcatca ccaacagttc gacgggggat gaaacaaaca tcgtcgccac cgatatcacg
    13561 ccgatggcga tgatatcaat gaggaacatt ttgcgccgtc cgactttatc ggaaatataa
    13621 ccaaacagcg atgtgccaac gaacagcccg gcgagcgttc ccgcgcccag caagccaatc
    13681 cagtcagcgt ccagttttag cgccggcgtc agttgctcca gcgccacgcc aatcattacc
    13741 agtatataac catccagaaa cggaccaccg cttccccaca gcaaaatgcg gcggtgaata
    13801 gaggaaaatt taagatcgtc aaagtttctg gacggttgca tgggttattc ctgttttttg
    13861 caatgagagt cagtgcggcg ttacgtatca aaccaacatc agccgtaacg gaactccaca
    13921 ccaaaggtgc cgcgcgggta ttcccactgt tccagcgccg accccagccc cagaatgcga
    13981 caggtgccgc actccagaca tccggcgtaa tcgaagcgca cactgccgtc atcctgcttc
    14041 ttgtacagac ctgcggggca cgctttcacc agcagctcca gcgcctgttt atcggcatcg
    14101 gctttcacaa caatgtgcgg atgctcttca tcgacattga atttattgac gcccagtttg
    14161 acgtccacat tgacgggaga agtcataaaa cggtcactcc tttcatgcca tccttgatca
    14221 gattgatgaa gcccactttc ttgccgtggc ggaggatttt cttgcgcatc agttccggcg
    14281 cactgccgtc aatggtgaac aggtcacgcg ccacgcctac cgccagttcc gggtagccgc
    14341 taaacatgcg tgggttatca aggaaggccg gtagtttctg gtacatacgc atatcgcgca
    14401 gcgggccact ctcaagatgc tgacgatatt ccgcgagttt ttgcttactg aaatcgtcgc
    14461 ttttcatcgc tgaaagcacg gtttttgctg cagcttcccc ggcggcaatc gccagatcca
    14521 taccgcgaat ggtaaaaccg aggttcatac acattccggc ggcatcaccg gcaatcaata
    14581 cgccgtcacc gaccagctcc ggcagcatgt tgatgcctgc ttccggcact acgtgagcgg
    14641 aatattccac cagcttgcca cccgcgatca gcggtgcaac ggccggatgt tgtttgaaat
    14701 cttccagcat ttgcggcacc gattttttcg cgtcatgcag atgatgcaga ccacaaacca
    14761 gccccagcga cagggtgttt tcattggtat aaagaaaacc gccgcccatc aggccatcgg
    14821 tgggtgatcc cgcaaatagg caagccgccc cctgattacc ctgcaactga aaacggtctt
    14881 caataaccga cttcggtaac tcgatcagtt ccttcacgcc aaccgccaca ttcgtcggtt
    14941 taacgcgttt tgccatcccc agcttttcgg caaggatgga gttcactcca tcagcaagga
    15001 tcaccgtttt cgcttcaatc acatcgccat cggcttctac accgacgact ttgccatcgc
    15061 gctgtacgag gttatcgacg cggatcccgg taattaactg cgcgcccgct tcttcggcct
    15121 gctccatcag ccaggcatca aatttactgc gcaaaacgga gtaagaacgc tgggatggcg
    15181 aggtttcgtc accgttgcag tagtccatag tcatcgctga cttttccgtc ataaacgcga
    15241 gtttttcatg ggtgatcagg cgttctaccg gggcggattc ggcgaaacca ggaataatgt
    15301 gttccaggct gtgggcatag agacgcccgc cggtgacgtt cttggcacct gcggaattgc
    15361 cacgctcgat aactaacact tgcgcccctt cgcgggcgag caccagtgcg gcaaccgaac
    15421 cggcaagccc tgcaccgacg atgatggcgt caaagatatc ttcggacata agagctccct
    15481 gtcagcggca ggaccaaaat gcagccctgc cagagtggat caacgcgcta aagctgcggt
    15541 cagcgccgga aggatcttca cggcgtcgcc aacaatgccg taatccgcgt actggaagat
    15601 cggcgcattt ttatctttat tgatggcgaa aatggtttgc gacgcgttag cgccaaccat
    15661 gtgctggatc tgcccggaga tccccacagc caggtacagt tcaggtttca gcatcaggtt
    15721 ggagataccg acatagcgtt cgtgctccat ccatttttcg ttttctgcca ccggacgaga
    15781 acaggccaac tccgcaccta tcgccttgca aagctgttct gccagcgcaa tgttctcttt
    15841 gctgccaata ccgcgaccga cgctgaccac caggcgggct ttgtcgagat cgacgctgtt
    15901 gctctggcgc gcctgggttg ccgtgcgggt aatcgccaca gccggagcct gccactccac
    15961 ggtgtgcgtt tcgccagtgc gtgacgcgtc tggctgagcc gcatcgaacg tgccgctgct
    16021 gatggtcagt accgcatacg gcgtggcaat gcgttcttcg ccaatcgcca gaccaccgta
    16081 aaccatatgt ttcactgttg ctttaccgtc ctgtacgctg acggtgctgg catcgttaga
    16141 caccgccgct ttaaggcgat aacccagttt tgccgccagt aatttgccgc gacgggtgtt
    16201 tggcaacagc accaggccgt ctgcaccgtg ctggcgaata gtgtcagcca tgacactggc
    16261 gtaatcttcg atcatccgat cgtccggttt gccgtttaat ttccagacat gattagcgcc
    16321 gagctggatt gcctgtgcgc cgtcggcatc attgaggaca aaggcattga tttgattagc
    16381 taaagcctgc gcaccgttca tcagttccgg cagacgagaa ggggtatcgc tgaataccca
    16441 gacttgagaa aacgtgttca tagcatcccc tgtaattaaa tgactttgcg aagattttca
    16501 gcaaatgcgg cgatctgttc ttcgccgtcg ccttcaatca cgatgcgctg acgctcgcgc
    16561 tgtttcggcg cggcaacctg ttgttctgac catgctgcct ctgcgttaaa accgatatcc
    16621 gccgccgacc atacctggac tggctttttc gccgcgccga gaatggcttt catcgaagga
    16681 atttgtgggg agttgatatc agtggaaaca gcaacaaccg caggcagtgg aatgcttaag
    16741 gtttcggttt catcttccag ttcgcgctca acggtgaggg tatctgccgt cagggagata
    16801 attttgctga cgccgttaac tgccggaata ttgaggattt cgcctaccag caaaccaacc
    16861 tgctgggcat aaaggtcgga agaaccatcg ccacagagga tcaaatcaaa gcctgctttc
    16921 tgggcggctg cagccagtgc gctcgccgtt tgttgcggca gtgcctgctc gaactggtca
    16981 tcaatcacca caatcagttc atccggaccg cgcgatagca catctttacg ccctttggcg
    17041 ttggtcaggg ctttaccgcc cacacttagg gccgtcacct gcgcctctgc tgcctgttgc
    17101 tttagctggc aagccgcttc aatagcgttg agatcgtatt ggcttatttt ggcatcggct
    17161 ttactgaagt ctaacgaacc atcagcatta ttgaccgcaa tatcctgttc atcaggcacg
    17221 cacttatagc aagtaataat cttcattgca tctccagaaa tcatgaagga aacgtcgttc
    17281 atatcggtgg tgttaattac agatatcatg ccctttaatt tggaaataaa actttaatca
    17341 ccaatattga aaatgtcaca cgcagataaa tccaactttc aatattgtta agttcctcac
    17401 caatattgaa aacacggcgt agcaaaaaga aattttcaat attgttttat ggatcaccaa
    17461 tattgaaagt cagatgctat ttttttactc gcaacataac ataacaaact gattaataat
    17521 caaaattaac gagaaaagcg ccctctattc acccgaaaca aaaatgtgat aaca
//
LOCUS       DAZKIP010000092        17256 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZKIP010000092 DAZKIP010000000
VERSION     DAZKIP010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17256
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.092"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(139..1497)
                     /gene="plaP"
                     /locus_tag="ACHOUK_003340"
     CDS             complement(139..1497)
                     /gene="plaP"
                     /locus_tag="ACHOUK_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFP9898728.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(1487..1549)
                     /gene="yoeI"
                     /locus_tag="ACHOUK_003341"
     CDS             complement(1487..1549)
                     /gene="yoeI"
                     /locus_tag="ACHOUK_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFP9898729.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(1764..2693)
                     /gene="yeeY"
                     /locus_tag="ACHOUK_003342"
     CDS             complement(1764..2693)
                     /gene="yeeY"
                     /locus_tag="ACHOUK_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9898730.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHNVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(2739..3563)
                     /gene="yeeZ"
                     /locus_tag="ACHOUK_003343"
     CDS             complement(2739..3563)
                     /gene="yeeZ"
                     /locus_tag="ACHOUK_003343"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9898731.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            3989..4039
                     /gene="hisL"
                     /locus_tag="ACHOUK_003344"
     CDS             3989..4039
                     /gene="hisL"
                     /locus_tag="ACHOUK_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFP9898732.1"
                     /translation="MTRVQFKQHHHHHHPD"
     misc_feature    4015..4138
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            4185..5084
                     /gene="hisG"
                     /locus_tag="ACHOUK_003345"
     CDS             4185..5084
                     /gene="hisG"
                     /locus_tag="ACHOUK_003345"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFP9898733.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDDAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTQRLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            5090..6394
                     /gene="hisD"
                     /locus_tag="ACHOUK_003346"
     CDS             5090..6394
                     /gene="hisD"
                     /locus_tag="ACHOUK_003346"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFP9898734.1"
                     /translation="MSFNTIIDWNSCTAKQQRQLLMRPAISASESITRTVNDILDSVK
                     ARGDDALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKVGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            6391..7461
                     /gene="hisC"
                     /locus_tag="ACHOUK_003347"
     CDS             6391..7461
                     /gene="hisC"
                     /locus_tag="ACHOUK_003347"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFP9898735.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIIAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            7461..8528
                     /gene="hisB"
                     /locus_tag="ACHOUK_003348"
     CDS             7461..8528
                     /gene="hisB"
                     /locus_tag="ACHOUK_003348"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFP9898736.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPQLLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTRRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            8528..9118
                     /gene="hisH"
                     /locus_tag="ACHOUK_003349"
     CDS             8528..9118
                     /gene="hisH"
                     /locus_tag="ACHOUK_003349"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFP9898737.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            9118..9855
                     /gene="hisA"
                     /locus_tag="ACHOUK_003350"
     CDS             9118..9855
                     /gene="hisA"
                     /locus_tag="ACHOUK_003350"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFP9898738.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAISCWQNA"
     gene            9837..10613
                     /gene="hisF"
                     /locus_tag="ACHOUK_003351"
     CDS             9837..10613
                     /gene="hisF"
                     /locus_tag="ACHOUK_003351"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFP9898739.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            10607..11218
                     /gene="hisIE"
                     /locus_tag="ACHOUK_003352"
     CDS             10607..11218
                     /gene="hisIE"
                     /locus_tag="ACHOUK_003352"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFP9898740.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(11315..12292)
                     /gene="wzzB"
                     /locus_tag="ACHOUK_003353"
     CDS             complement(11315..12292)
                     /gene="wzzB"
                     /locus_tag="ACHOUK_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFP9898741.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKELRIRQIQEALQYANQAQVAKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(12438..13604)
                     /gene="ugd"
                     /locus_tag="ACHOUK_003354"
     CDS             complement(12438..13604)
                     /gene="ugd"
                     /locus_tag="ACHOUK_003354"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFP9898742.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELRDVADKVYTRDLFGSD"
     gene            complement(13853..15259)
                     /gene="gndA"
                     /locus_tag="ACHOUK_003355"
     CDS             complement(13853..15259)
                     /gene="gndA"
                     /locus_tag="ACHOUK_003355"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFP9898743.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQI
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(15460..16125)
                     /gene="perB"
                     /locus_tag="ACHOUK_003356"
     CDS             complement(15460..16125)
                     /gene="perB"
                     /locus_tag="ACHOUK_003356"
                     /EC_number="2.3.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-perosamine N-acetyltransferase"
                     /protein_id="HFP9898744.1"
                     /translation="MNLYGIFGAGSYGRETIPILNQQIKQECGSDYALVFVDDVLAGK
                     KVNGFEVLSTNCFLKAPYLKKYFNVAIANDKIRQRVSESILLHGVEPITIKHPNSVVY
                     DHTMIGSGAIISPFVTISTNTHIGRFFHANIYSYVAHDCQIGDYVTFAPGAKCNGYVV
                     IEDNAYIGSGAVIKQGVPNRPLIIGAGAIIGMGAVVTKSVPAGITVCGNPAREMKRSP
                     TSI"
     gene            complement(16169..17100)
                     /locus_tag="ACHOUK_003357"
                     /pseudo
     CDS             complement(16169..17100)
                     /locus_tag="ACHOUK_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         4506 a         4334 c         4165 g         4251 t
ORIGIN      
        1 atgactggtg gaatgcagac caataaccaa attctctaaa ttagaacaaa tggttattaa
       61 tgaggatttc gataaaaaag aataaaaaaa ccggagccaa atgttcattc gactccggtt
      121 tattattaga aatgatgatt atgctacgtc ttcgtactgc ggaaccgggt tacggaagct
      181 cttggtgacg caagccaggt aaatcagacc gatagcagcc cagatcagac ccagaaccat
      241 tgagctttct tccaggttaa cccacagcgc accaacggtc agcgcaccac acatcggcag
      301 gaacagatac tggaagtgat ctttcagcgt cttattacgc ttctcacgga tccagaactg
      361 cgagatgacc gacaggttaa cgaaggtgaa cgccaccaac gcaccaaagt tgatcagcgc
      421 cgtagccatt accaggtcga agttgattgc cagcagggca atcgcgccaa ccaggatgat
      481 gttcatcgcc ggagtacgcc atttcgggtg tacataaccg aagaaacttt tcgggaacac
      541 gccgtcacga cccattacgt acatcagacg cgctacgcct gcgtgcgccg ccataccgga
      601 cgccagtacg gtaatggtgg agaagatcag cgcaccgacc tggaacgctt tacccgcaac
      661 gtacagcatg atttcaggct gtgacgcatc cggatcttta aagcgagaga tatccgggaa
      721 gtacagctgc aggaagtaag ttgcaaagat gaagatcatg ccgccgatca gcgcggtcag
      781 gaaaatcgca cgcgggatca cgcgctctgc atctttggtt tcttccgaca gattgctgat
      841 gccgtcaaag ccggtaaagg agaagcacag gattgtcgcc ccggtaatca tcgggataac
      901 atgcgcatcg ccagaccaga atggacgagt gctcgccagc gtaccagcgc cttcaccttc
      961 aaatacgcca taaacaacca tgcccagaat caccgcgatc agcactacct gcaacacgac
     1021 gattacagtg ttgaagttcg ctacggattt cagactacgc aggttaaagg cggtcataaa
     1081 ggccaccagc gccaccacaa acatccacga tgggatggaa ggcaccagag cttcaaaata
     1141 gattttcgcc agcagaatgt tgatcatcgg cgcgaacaga tagtcgagca gagaagacca
     1201 acccaccata aagccgacag tcgggctaat ggatttctgg gcgtaagtat atgcagagcc
     1261 agcagaagga tagcggcgaa ccagcttccc gtagctcaga gccgtaaaca ggatcgcaat
     1321 caatgcgaac gcataggctg tcggcacatg accatccgtg aggcctgaaa cgataccaaa
     1381 agtatcaaac agcgtcatcg gctgcatata ggcaagaccc atcattacaa ccggaactaa
     1441 cgtaagcgtt ttacgcaatt ccacgcgaga ggtgtttgga gtaacgttat gcgacatggt
     1501 cattctcctt tacggtgata accgtcgcgt aagcaaaaaa ttgccccatt tttttggatt
     1561 cctcagcgac aacaactgtc gatttttagt aaatatctat ccggtacgaa gcccggcctc
     1621 ttggtatgaa tgattggttt gaagcaaaaa ataaccgacg ctgatgaaac gtcggttttt
     1681 agtcattttt tgacagcggc gcattgtgcc taaatggggg ggaaatgaca agagaatgag
     1741 aggcttgtca taataatttt tctttaaatg gctgattttc cgtcatcaga ttttgcataa
     1801 acaccggcga tcgcactatt tgctaaaatt tcatcccgcc accagccgct ggcaagtcct
     1861 gcggtctgtt cattccagcc aatccagacg ggttcaaaga gagtttgggc gaccactttc
     1921 ttctcgatta acgcgccact atcgagaaaa cgttgcgcca gataacgggg taaatagccg
     1981 cagccgaggc cgctaatttg cagctccagc ttggttttaa aatcgaaaac ggtaatggct
     2041 tcttgttcgt cgagaagttg tgaggccgta gaagcgcctg cctgcgcagt gtcccccacc
     2101 acaatcgccc gatatcgttt aatgatacga cggtttaatg gttcttcttc ctgggctaac
     2161 ggatgatgag gcgcgaccgc aaagacttgt tccagatcgc ccaaccgcga gaaaccaaac
     2221 tcactggaag atggtggctc atgcatcgcg ccaacaataa tatccgctcg cccctgagtc
     2281 agggcatccc atgaaccacc gagtacgcca ttgataaact tcaggcgcgt cacgttatga
     2341 tgttgataaa aagcttcaat gagtggcgca agcagagaaa aaggaaacgt gtcgtccacg
     2401 ccaatcacca gctcgttttc ccagccttca tgcagtttga tcgcctgctt ttccagttct
     2461 cgcacggtat gcagaacttc ccgccccttc tctaataaca tttttccggt acgggtgaat
     2521 ttagcgcggt gaccgctacg atcaagcaat tgaatattaa ggtcgctttc cagcttgtga
     2581 acggtgtaac tcagagcgga cggcgtcttg tagagtttag ccgacgccgc cgcaaaactg
     2641 ccttcttttt ctaaggcatc gaggatcatc aacacatcca gcagtggttt catacacgcc
     2701 cccttgcagt gcatgcggcg tcctgctggc gtgatcgttt actccagcgg cattaccagc
     2761 ggatcgggat actggtattc aaatcccagt tcattacaaa tccgactgcc atcaataatc
     2821 ttacctttgc cgctgtccag actatttctg aattgcggag gttccagccc cagtaaacgg
     2881 gccatctgcg gatagaaaac attacgcgca gggtgagctg gcgcacatat attatagatg
     2941 tgtccgcctt taggtgcctg taacaacaga gtgatagcgc caataacatc ttctaaatgg
     3001 actaaattaa caccatgttc accatcaggc gcggtttttc cggcaaagaa gcgtccggga
     3061 tgacgccccg gtccgaccag gcccgcaaga cgcagaatat cgaccgaagt accgggtaaa
     3121 ttgtgcagcc agtcttcgag ttcttctaat actcgtccac tgttggtcac cggattacgc
     3181 ggggtggttt ctttcaccgt gccttgcgcg tcgccataga cagacgttga actggtaaaa
     3241 ataattcggg gaatacgatg ggccagcgcg ctatccacta actcttgtac cgcttgtaaa
     3301 tagaactcat cgccggggcc gctacgacgg gccggaagcg taatgaccag cgcatcggca
     3361 tccatcaggg catccagatc gtcagaatcg caaactaact caggctccat gcgaagcaga
     3421 tagctatcaa tgccactcat tcgggccgct tcgacaccat cttgtgtggt tttactcccg
     3481 gtgacttgcc agcctcgcgc tgaaagtgac atcgccagcg gcatgcctaa ccaccctaac
     3541 ccgacaattg cgaccttttt catccgttat ctcctgatac accttagatc tataaggcta
     3601 cgctagcgta tcaaaactga caattcattc tatgaatgaa atcgaaactg ttagcaaata
     3661 taccattgcc tgatgcgctc cgcttattag tcctacaaaa catcaatata ttgaatttag
     3721 gcaacatttg tagaccagat aaggcattca cgccgcatcc ggcaggaaca aagcccactt
     3781 tagttacaga acacaatgtt cttgagggtt ttaatgacat attttcacga ttactttttt
     3841 aacctagtca ctcttagccc cctcctttcc ccgctcattc attaaacaaa tccatcgcca
     3901 taaaatatat aaaaaagccc ttgctttcta acgtgaaagt ggtttaggtt aaaagacatc
     3961 agttgaataa acattcacag agacttttat gacacgcgtt caatttaaac agcaccatca
     4021 tcaccatcat cctgactagt ctttcaggcg atgtgtgctg gaagacattc agatcttcca
     4081 gtggtgcatg aacgcatgag aaagcccccg gaagatcacc ttccgggggc ttttttattg
     4141 cgcggttgat aacggttcag acaggtttaa agaggaataa gaaaatgaca gacaacactc
     4201 gtttacgcat agctatgcag aaatccggcc gtttaagtga tgactctcgc gaattgctgg
     4261 cgcgctgtgg cattaaaatc aatcttcaca cccagcgcct gatcgcgatg gcagaaaaca
     4321 tgccgattga tattctgcgc gtgcgtgacg acgacattcc cggtctggta atggacggcg
     4381 tggtagatct tgggattatc ggcgaaaacg tgctggaaga agagctgctt aaccgccgcg
     4441 cccagggtga agatccacgc tactttaccc tgcgtcgtct ggatttcggc ggctgccgtc
     4501 tttcgctggc aacgccggtt gatgatgcct gggacggccc gctctcctta aacggtaaac
     4561 gtatcgccac ctcttatcct cacctgctca agcgttatct cgaccagaaa ggcatctctt
     4621 ttaaatcctg cttactgaac ggttctgttg aagtcgcccc gcgtgccgga ctggcggatg
     4681 cgatttgcga tctggtttcc accggtgcca cgctggaagc aaacggcctg cgcgaagtcg
     4741 aagttatcta tcgctcgaaa gcctgcctga tacaacgcga tggcgaaatg gaagaatcca
     4801 aacagcaact gatcgacaag ctgctgaccc gtattcaggg tgtgatccag gcgcgcgaat
     4861 caaaatacat catgatgcac gcaccgaccc aacgtctgga tgaagtcatc gccctgctgc
     4921 caggtgccga acgcccaacc attctgccgc tggcgggtga tcaacagcgc gtagcgatgc
     4981 acatggtcag tagcgaaacc ctgttctggg aaacgatgga aaaactgaaa gcgctgggtg
     5041 ccagttcaat cctggtcctg ccgattgaga agatgatgga gtgatcgcca tgagctttaa
     5101 cacaatcatt gactggaata gctgtactgc aaagcaacaa cgccagctgt taatgcgccc
     5161 ggcgatctcc gcttctgaaa gcattacccg cactgttaac gatattctcg atagcgtgaa
     5221 agcacgcggt gatgacgccc tgcgggaata tagcgcgaag tttgataaaa ccacggttac
     5281 cgcactgaag gtgtctgctg aggaaattgc cgccgccagc gaacgcctga gcgacgagct
     5341 aaaacaggcg atggcggtgg cagtaaagaa tattgaaacc ttccacactg cgcaaaaact
     5401 gccgccggta gatgtagaaa cgcagccagg cgtgcgttgc cagcaagtca cgcgcccggt
     5461 agcttcagtt gggttgtata ttcctggcgg ctccgccccg ctcttctcaa cggtattaat
     5521 gctggcaact ccggcgcgta ttgcgggctg taaaaaagtg gtgttgtgct caccgccgcc
     5581 gattgccgat gagatccttt atgcggcgca gctgtgcggt gtgcaggacg tgtttaacgt
     5641 cggcggcgca caggccattg ccgcgctggc gtttggtacg gaatctgtgc cgaaagtgga
     5701 caaaatcttc gggccgggta acgcctttgt caccgaggca aaacgtcagg tgagccagcg
     5761 tctggacggt gcggcgatcg atatgcccgc aggcccgtcg gaagtgttgg tcattgccga
     5821 cagcggcgca acgccggatt tcgtggcttc tgatttgctt tctcaggctg aacacggccc
     5881 ggactcacag gtgattttac tgacgcctga cgctgatatg gcgcatcaag ttgccgaagc
     5941 cgtcgaacgc cagttagcag aactgccgcg tgccgaaacc gcccgccagg cactgaacgc
     6001 cagccgcctg atcgtgacta aagatttagc gcagtgcgtg gagatctcca accagtacgg
     6061 cccggagcac ctgatcattc agacccgcaa cgcccgcgaa ctggtcgatg gcatcaccag
     6121 cgccggttcg gtatttcttg gtgactggtc accggaatcg gcaggtgatt acgcctccgg
     6181 aaccaaccat gttctaccga cttacggcta caccgccacc tgttccagcc tcggactggc
     6241 ggatttccag aagcggatga ccgtgcagga actgtcgaaa gtaggtttct ccgcgctggc
     6301 ttcgaccatt gaaacactgg ccgccgccga gcgcctgacc gcccacaaaa atgccgttac
     6361 tttgcgtgtt aacgccctta aggagcaagc atgagcaccg tgactattac cgatttagcg
     6421 cgtgaaaacg tccgcaacct gacgccgtat cagtcggcgc gtcgtctggg cggtaatggc
     6481 gacgtctggc tgaacgccaa cgaatatccc acagccgtgg agtttcagct tactcagcaa
     6541 acgctcaacc gctacccgga atgccagccg aaagcggtga ttgaaaatta cgcgcaatat
     6601 gcaggcgtaa aaccggaaca ggtgctggtc agccgtggcg cggacgaagg tattgaacta
     6661 ctgattcgcg ctttttgcga accgggtaaa gacgccatcc tctactgccc gccaacgtac
     6721 ggcatgtaca gcgtcagcgc tgaaaccatt ggcgtcgagt gccgcacagt gccgacgctg
     6781 gaaaactggc aactggactt acagggcatt tccgacaagc tggacggcgt aaaagtggtc
     6841 tatgtttgca gccccaacaa cccaaccggg caactgatca atccgcagga ttttcgcacc
     6901 ctgctggagt taacgcgcgg taaggcgatt gtggttgccg atgaagccta tatcgagttt
     6961 tgcccgcagg catcgctggc tggctggctg gcggaatatc cgcacctggc tattttgcgc
     7021 acactgtcga aagcttttgc tctggcgggc cttcgttgcg gatttacgct ggcaaacgaa
     7081 gaagtcatca acctgctgat gaaagtgatt gccccctacc cgctctcgac gccggttgcc
     7141 gacattgcgg cccaggcgtt aagcccgcag ggaattatcg ccatgcgcga acgggtggcg
     7201 caaattattg cagaacgcga atacctgatt gccgcactga aagagatccc ctgcgtggag
     7261 caggtttttg actctgaaac caactacatt ctggcgcgct ttaaagcctc cagtgcagtg
     7321 tttaaatctt tgtgggatca gggcattatc ttacgtgatc agaataaaca accctcttta
     7381 agcggctgcc tgcgaattac cgtcggaacc cgtgaagaaa gccagcgcgt cattgacgcc
     7441 ttacgtgcgg agcaagtttg atgagtcaga agtatctttt tatcgatcgc gatggaaccc
     7501 tgattagcga accgccgagt gattttcagg tggatcgttt tgacaaactc gcctttgaac
     7561 cgggcgtgat cccgcaactg ctgaagctgc aaaaagcggg ctacaaactg gtgatgatca
     7621 ctaatcagga tggtctggga acacaaagtt tcccgcaggc ggatttcgat ggcccgcaca
     7681 acctgatgat gcagatattc acctcgcaag gcgtacagtt tgatgaagtg ctgatttgtc
     7741 cgcacctgcc cgccgatgag tgcgactgcc gtaaaccgaa agtaaaactg gtggagcgtt
     7801 atctcgctga gcaagcgatg gatcgcgcca acagttatgt gattggcgat cgcgcgaccg
     7861 atattcaact cgctgaaaac atgggcatta atggtttacg ctacgaccgc gaaaccctga
     7921 actggccaat gattggcgag caactcacca gacgtgaccg ttacgctcac gtagtgcgta
     7981 ataccaaaga gacgcagatt gacgttcagg tgtggctgga tcgtgaaggt ggcagcaaga
     8041 ttaataccgg cgttggcttc tttgatcaca tgctggatca gatcgccacc cacggcggtt
     8101 tccgtatgga aatcaacgtc aaaggcgacc tctatatcga cgatcaccac accgtcgaag
     8161 ataccggcct ggcgctgggc gaagcgttaa aaattgccct cggcgataaa cgcggtattt
     8221 gccgctttgg ttttgtgctg ccgatggacg aatgccttgc ccgctgcgcg ctggatatct
     8281 ctggtcgccc gcacctggaa tataaagccg agtttaccta ccaacgcgtg ggcgatctca
     8341 gcaccgagat gatcgagcac ttcttccgtt cgctctctta caccatgggc gtgacgctcc
     8401 acctgaaaac caaaggtaaa aacgatcatc accgtgtaga gagcctgttc aaagcctttg
     8461 gtcggaccct gcgccaggcc atccgcgtgg aaggcgatac cctgccctcg tcgaaaggag
     8521 tgctgtaatg aacgtggtga tccttgatac tggctgcgcc aacctgaact cggtgaagtc
     8581 tgccattgcg cgtcacggtt atgaacccaa agtcagccgt gacccggacg tggtgttgct
     8641 ggccgataaa ctatttttac ccggcgttgg cactgcacaa gcagcgatgg atcaggtgcg
     8701 tgagcgcgag ctgtttgatc tcatcaaagc ctgtacccaa ccggtgctgg gcatctgctt
     8761 agggatgcaa ctgctggggc ggcgcagtga agagagcaac ggcgtcgatt tgctgggcat
     8821 catcgacgaa gacgtgccga aaatgaccga ctttggtctg ccgctgccgc atatgggctg
     8881 gaatcgcgtt tacccgcagg caggcaaccg cctgtttcag gggattgaag acggcgcgta
     8941 cttttacttt gttcacagct acgcaatgcc agtcaatccg tggaccatcg cccagtgtaa
     9001 ttacggcgaa ccattcaccg cggcggtaca aaaagataac ttctacggcg tgcagttcca
     9061 tccggagcgt tctggtgccg ctggcgctaa gttgctgaaa aacttcctgg agatgtgatg
     9121 attattccgg cattagattt aatcgacggc actgtggtgc gtctccatca gggcgattac
     9181 ggcaaacaac gcgattacgg taacgacccg ctgccgcgct tacaggatta cgccgcacaa
     9241 ggtgccgaag tgctgcattt ggtggatctg accggggcaa aagatccggc aaaacgtcaa
     9301 atcccgctga ttaaaaccct ggtcgctggc gttaacgttc cggtgcaggt tggcggcggc
     9361 gtgcgtagcg aagaagacgt ggcggcgtta ctggaagctg gggttgcgcg cgtggtggtt
     9421 ggctccactg cggtgaaatc accagaaatg gtgaaaggct ggtttgaacg tttcggtgcc
     9481 gatgccttag tgctggcgct ggatgtccgt attgacgagc aaggcaacaa gcaggtggcg
     9541 gtcagcggct ggcaagagaa ttcgggcgtg tcactggaac aactggtgga aacctatctg
     9601 cccgtcggcc tgaaacatgt gctgtgtacc gatatctcgc gcgacggcac gctggcaggc
     9661 tctaacgtct ctttatatga agaagtgtgc gccagatatc cgcaggtggc atttcagtca
     9721 tccggcggca ttggcgacat taacgatgtt gctgccctgc gtggcactgg cgtgcgcggc
     9781 gtaatagttg gtcgtgcatt actggaaggt aaattcaccg tgaaggaggc catctcatgc
     9841 tggcaaaacg cataattcca tgtctcgacg ttcgtgatgg tcaggtggta aaaggcgtac
     9901 agtttcgcaa ccatgaaatc attggcgata tcgtgccgct ggcaaaacgc tacgccgaag
     9961 aaggtgcaga cgaactggtg ttctacgata tcaccgcttc cagcgatggc cgtgtggtag
    10021 ataaaagttg ggtatctcgc gtggcggaag tgatcgacat tccgttctgc gtggcgggtg
    10081 ggattaagtc tctggaagat gccgcgaaaa ttctttcctt tggcgcggat aaaatttcca
    10141 tcaactcccc tgcgctggcg gacccgacgt taattactcg cctggcggat cgatttggcg
    10201 tgcagtgtat tgtggtgggt attgatacct ggtacgacgc tgaaaccggt aaatatcatg
    10261 tgaatcaata taccggcgat gaaagccgca cccgcgtcac tcagtgggaa acgctcgact
    10321 gggtacagga agtgcaaaaa cgcggtgccg gagaaatcgt cctcaacatg atgaatcagg
    10381 acggcgtgcg taacggttac gacctcgaac aactgaaaaa agtgcgtgaa gtttgccacg
    10441 tcccgctgat tgcttccggt ggcgcgggca ccatggaaca cttcctcgaa gccttccgcg
    10501 atgccgacgt tgacggcgcg ctggcagctt ccgtattcca caaacaaata atcaatattg
    10561 gtgaattaaa agcgtacctg gcaacacagg gcgtggagat caggatatgt taacagaaca
    10621 acaacgtcgc gaactggact gggaaaaaac cgacggactg atgccggtga ttgtgcaaca
    10681 cgcggtatcc ggcgaagtgt taatgctggg ctatatgaac ccggaagcct tagacaaaac
    10741 catcgaaagc ggcaaagtca ccttcttctc gcgcactaaa cagcgactgt ggaccaaagg
    10801 cgaaacgtcg ggcaatttcc tcaacgtagt aaatattgcg ccggactgcg acaacgacac
    10861 gctactggta ctggcgaatc ctatcggacc aacctgccac aaaggcacca gcagctgctt
    10921 cggcgacacc gctcaccagt ggctgttcct gtatcaactg gaacaattgc tcgccgaacg
    10981 caaatctgcc gacccggaaa cctcctacac cgccaaactg tatgccagcg gcaccaaacg
    11041 cattgcgcag aaagtgggcg aagaaggcgt ggaaaccgca ctggccgcga ctgtacatga
    11101 ccgctttgag ctgaccaacg aggcgtctga cttgatgtat cacctgctgg tattgttgca
    11161 ggatcaggat ctggatttaa caacggtgat tgagaacctg cgtaaacgtc atcagtgagt
    11221 tgcggggtaa gcggatgcga tattgttgtc gcatccggca aaaaaaacgg gcaaggtgtc
    11281 accaccctgc cctttttctt taaaaccgaa aagattactt cgcgttgtaa ttacgcagag
    11341 cgttacgccc cagcacaatc cccgcgccaa ccatgccgcc cagcagcacc gccagaatca
    11401 aggtgattgc ctttttcgga ctatcgcgac gaataggtaa cgtcggtttc attacatagc
    11461 ggtaagcatg aatatcaaga tcatcaacct ttaagttttc aatatccaac aggttttgac
    11521 gtgtctgata gtagtttggt gagaacacca acggacgggt cgcctcatgc ttaatcatcg
    11581 actccagcgc ttcgctcccc aaaaggaaca acgtatcttg cgtcacatct tcagtctgct
    11641 gaacctgtgg ctttgccacc tgcgcctgat tcgcatactg caacgcttcc tgaatctgac
    11701 ggatacgcag ctctttctgc tcctgcgcaa ctacttcctg cgttcttaaa gagtcctgca
    11761 agtttttccg tcccagagca atgttgtctt tgagatcctt ttctagctct tgattcactt
    11821 tatcatcaac ttgctgaatg tattgggcca acttcatttg tgctccctct gcagtttgcc
    11881 caacataaga aacagtcaat ggtaattgct ggttcttaac agaaggttcg atggtaagtt
    11941 tttctggttc ttcctgatta tccagcgttt ctgctaatgc tgagaaggca gaactgaagc
    12001 gaccaatcaa cgtctcctgc aaatccgata ctttcggtgc agcctgacca tagataacat
    12061 tcatggcatt gttatagcca gcaatttgcc ccacgtcggg ctgagtgata attgctgttg
    12121 acgtccattt ctccttcgct actgccaaat aaccaatagc cagagcaata gccacaatga
    12181 cggaaatgat gattgtcatc ttgccacgcc acaactgcac tagtaaatca atcaaatcaa
    12241 tctgttccgg gtcatggttt tgcccagaaa cattattatt ttctactctc atcattaccc
    12301 taactgacgg aaatttccaa gtccgcatag tttagcgata atccctgtga atgctatagg
    12361 ataagtgata aaaataatga attattgcgg aggtaacaaa agacaatcag ggcgtaaata
    12421 gccctgataa caagatgtta gtcgctgcca aagagatcgc gggtgtagac cttatctgcc
    12481 acatccctaa gctcttctgc catacggttg gaaataatga cgtcggcttg ttgtttgaag
    12541 gtggcgagat cacgttccag gcgagagttg aagaatgagt cttctttcat cactggctcg
    12601 tagatgatta cttcaacacc tttcgccttg atacgcttca taatcccctg aatagaagac
    12661 gcacggaagt tatctgaacc gctcttcata atcagacgat aaatacctac cacttgcggc
    12721 ttgcgtgaca aaatggcatc ggcaataaaa tctttacgcg tgcggttagc atcgacaatt
    12781 gccgagatca ggttattcgg cacagactgg tagttcgcca gtaactgctt ggtatctttc
    12841 ggcaaacaat aaccaccata accaaacgac ggattgttgt aatggttgcc aatacgtggg
    12901 tcgagacaaa cgccttcgat tatttgacgg gaattcagac ctaaactttc tgcgtagcta
    12961 tccagttcgt taaagtacgc cacgcgcatc gcgaggtagg tgtttgcaaa aagtttaatc
    13021 gcttctgctt cagtggagtc ggtaaacagg gttgggatat tttgcttaat cgcgccttcc
    13081 tgtaataacg cagcaaaacg ttctgcgcgt tccgaacgct caccgattac aatacgtgac
    13141 ggatgaaggt tatcgtaaag agctttacct tcgcggagaa attccggaga gaaaataata
    13201 ttttcagtac gatatttctt atgcatcgct tcggtaaaac caacgggaac cgttgatttg
    13261 atgaccataa ccgcataagg atttatctca actacgtctt taattactga ttctacactg
    13321 gatgtattga aataattagt tttaggatca tagtcggttg gagtggcgat gatgacataa
    13381 tcagcatccc ggtaggcttc atttttatct aatgtggcat taaagtgtat tttatctgat
    13441 tgcaaaaact gctgaatttc cttatcaaca ataggagata tccgatcatt cagcatagca
    13501 acgcgtgacg gtaaaatatc taatgccaca acctcatgat tttgagcgat tagaagcccg
    13561 tttgacaagc ctacatagcc agtaccggaa atggtgattt tcatttcatg ctctcagaat
    13621 taacttaact gtgaatcatg atgtttttaa catcctgata agagctaaaa gttttaacgc
    13681 tacgttgttc gatggtcaac gcagcgaaaa acaattcaga ttttctttat aagaatagct
    13741 gatatttatt aagttaatat taagcagtga tatttagtct ataagataat tggcaaaaaa
    13801 aagcccggta caatatacgc cgggcctcaa ttttattgtt gattaaatca gattaatcca
    13861 gccattcggt atggaacaca ccttctttat caatgcgctt ataagtatgc gcaccgaaat
    13921 agtcacgctg tgcctggatc aggttcgcag gcagaacagc ggcgcggtag ctgtcataat
    13981 aggcaaccgc agcggcgaag gtcggaaccg ggataccgtt ctgtaccgca taagcgacga
    14041 catcgcgcag cgcctgctgg tagtcatcgg caatttgctt gaagtaagga gccagcagca
    14101 ggttagcgat ctgcggattt tcggcataag catcggtgat tttctgcagg aactgcgcac
    14161 ggatgatgca gccagcacgg aaaatcttcg cgatttcgcc gtagttcaga tcccagttgt
    14221 actcttcaga cgccgcacgc agttgagaga acccctgagc gtaagaaacg attttgccca
    14281 gatacagtgc acggcgaact ttttcgatga actcagcctt gtcgccagct ggctgcgctt
    14341 gtgggccaga gagaacttta gacgcagcaa cgcgctgatc tttcagagaa gagatgtatc
    14401 gtgcaaacac agactcggta atcagcgaca gcggttcgcc gagatccagt gcgctctggc
    14461 tggtccattt gcccgtacct ttgtttgccg cttcatccag gatcacatca accaggtagt
    14521 taccgtcttc atctttttta gtgaagatgt ctttggtaat gtcgatcagg tagctgctca
    14581 gttcaccgtt attccactcg gtaaagatct gcgccagttc ttcgttggtg aggttcagac
    14641 cacctttaag cagagaatag gcttcagcaa tcagctgcat atcgccgtat tcaataccgt
    14701 tgtgaaccat cttcacatag tgacctgcgc catcggcacc aatataggta acgcatggct
    14761 caccgtcttc agccactgcg gcgattttgg tcaggatcgg cgcaactagt tcataggctt
    14821 ctttctgccc accaggcata atggaaggac cttttagtgc gccctcctca ccaccggaaa
    14881 caccggtacc gatgaagtta aagccttctg cagaaagctc acggttacga cgaatggtgt
    14941 cctggaagaa ggtattacca ccatcaatga tgatgtcacc tttatcgagg tatggcttaa
    15001 gggaatcaat agcagcatcc gtgcctgcac ctgctttcac cattaacaag atgcgacgag
    15061 gcgtttccag agattcaaca aattctttca ccgtatagta aggaaccagt ttcttgcctg
    15121 gattttcggc aatcacttct tccgtctttt cacgggaacg gttgaaaata gagacggtat
    15181 aaccacggct ttcgatgttg agcgcaaggt tgcgccccat cactgccata ccgactacgc
    15241 cgatctgttg ctttgacatt gtttactcct gtcaggggtt accgctagat atgcggcttg
    15301 aataacgtat aaaaatgtat cagagtatta taaatttcat gctgtatcag taataactaa
    15361 gtaaaggata gcttaaaaag aagccataat ttagtaaatt agcgaaatta tatatatttt
    15421 aaacattagt cccatttgga acgtgttttc gcattcccat taaatagatg ttggcgatct
    15481 tttcatttct cttgctggat ttccgcacac agttataccg gcaggaacac ttttagtgac
    15541 aacagccccc atacctataa tggctcccgc gccaataata agtgggcgat taggaacacc
    15601 ctgcttaatt actgcacccg agcctatata tgcattgtct tcaataacaa catatccatt
    15661 acatttagcc ccaggagcaa atgtaacata gtctcctatt tgacaatcat gtgcaacgta
    15721 tgagtatatg tttgcatgaa aaaacctccc tatatgagta ttagtagata ttgtaacaaa
    15781 gggagaaata atagcgccac tacctatcat agtatgatca taaacaacgc tatttggatg
    15841 ttttatagtt attggttcaa ccccgtgtaa taatattgac tcagacactc tctgtcgtat
    15901 cttatcatta gcaatagcaa cattaaaata cttttttaaa taaggggctt ttagaaagca
    15961 gttggttgaa agcacttcaa aaccattaac tttctttcct gccaaaacat catccacaaa
    16021 aaccagagca tagtcagaac cacattcttg ctttatttgt tgatttagaa tgggtattgt
    16081 ttctctacca taacttccag caccaaaaat accatacaaa ttcattttcc catctccaaa
    16141 gaaatatctt atcaaaacca atgcaagatt atgaaagccc gcacgctgca atgcctgacg
    16201 ctaggaagtt tctgtccatg actgcttttc gcatcttaca tgataatcct gccttgaata
    16261 cattatcatt gtcagatatt aatagcgatg tgccgcattc tagaaaatca ttcgaatgca
    16321 actcgccttc ttatattata gtcgatttca ttcattacca catcaggcta ctgtgcaact
    16381 tattctccat gcgccagtga tttcgattta ctgtggcgaa cttctctgcg gttaaagcag
    16441 cagatctaat ataatatttg atcgtcattt cggattcttt cttttgctct gctattattg
    16501 agagaaagtg gactgccatt cataaattct gcagcccttt ccattcaaac gtgaaatcaa
    16561 taagctcatc aggagcatct caaacaatat gaagacggac atcgtctctg ccgtgcctct
    16621 tttcactaat tgcgtaactg tcatgttttg gattatttaa ttctttcagc gtaaatatct
    16681 cctcaaagac tctattaagc cgactcttat ttccttttac agcgaataaa taatcacatc
    16741 tctgttttta tatcttctct gcaatatctt tctggcaagc catcgcatca gttatgatta
    16801 tttttccttt aatacccatc attttagaaa gttcatgaat aactgtgaac gcattaggtt
    16861 tctcatccgt cttgatctat ccgaggacca gactgtgcat tgctgaaacg cactaatgac
    16921 atgaaccact cctctgcgac gactcttgtc ataagtgtgc cgaagtgttt taccatcaat
    16981 tgcgatgacg tcgttatcat ctggaaaatg gcagacatgt attcagttaa caaagcactc
    17041 gtgaaatatt gcaggactga tgcaggatac aactttggct ataatatcgt aaacagggat
    17101 aataaaaatc tattgtctca attattttaa gttccatttt gttctccttt ttatataagg
    17161 cacatttgtt attacataag cgatgaaaaa atgtcttaag caaaggaacg tctcaatcgt
    17221 gacgtgaaat gcaaattggt cagtacaaaa ctttat
//
LOCUS       DAZKIP010000093        16766 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZKIP010000093 DAZKIP010000000
VERSION     DAZKIP010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.094"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            208..453
                     /locus_tag="ACHOUK_003358"
     CDS             208..453
                     /locus_tag="ACHOUK_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HFP9898745.1"
                     /translation="MCDTKRSIGRKCESGLAANVPLRGVFVQDYDSHTQPKLTNRRIQ
                     MDAQTRRRERRAEKQAQWKADRILASTFRRNATKRHY"
     gene            398..1318
                     /locus_tag="ACHOUK_003359"
                     /pseudo
     CDS             398..1318
                     /locus_tag="ACHOUK_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(1333..1408)
                     /locus_tag="ACHOUK_003360"
     tRNA            complement(1333..1408)
                     /locus_tag="ACHOUK_003360"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1373..1375),aa:Thr,seq:cgt)
     gene            complement(1523..2776)
                     /gene="proA"
                     /locus_tag="ACHOUK_003361"
     CDS             complement(1523..2776)
                     /gene="proA"
                     /locus_tag="ACHOUK_003361"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFP9898746.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVVVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIAYSFLPALSKQ
                     MAESGVTLHADASALAQLQTGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(2788..3891)
                     /gene="proB"
                     /locus_tag="ACHOUK_003362"
     CDS             complement(2788..3891)
                     /gene="proB"
                     /locus_tag="ACHOUK_003362"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFP9898747.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            4179..5234
                     /gene="phoE"
                     /locus_tag="ACHOUK_003363"
     CDS             4179..5234
                     /gene="phoE"
                     /locus_tag="ACHOUK_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFP9898748.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(5273..5674)
                     /gene="crl"
                     /locus_tag="ACHOUK_003364"
     CDS             complement(5273..5674)
                     /gene="crl"
                     /locus_tag="ACHOUK_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFP9898749.1"
                     /translation="MTLPSGHPKSRLIKKFTVLGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5732..6976)
                     /gene="frsA"
                     /locus_tag="ACHOUK_003365"
     CDS             complement(5732..6976)
                     /gene="frsA"
                     /locus_tag="ACHOUK_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFP9898750.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSCLLHQRVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(7068..7526)
                     /gene="gpt"
                     /locus_tag="ACHOUK_003366"
     CDS             complement(7068..7526)
                     /gene="gpt"
                     /locus_tag="ACHOUK_003366"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFP9898751.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            7787..9244
                     /gene="pepD"
                     /locus_tag="ACHOUK_003367"
     CDS             7787..9244
                     /gene="pepD"
                     /locus_tag="ACHOUK_003367"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFP9898752.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(9301..9822)
                     /gene="prfH"
                     /locus_tag="ACHOUK_003368"
                     /pseudo
     CDS             complement(9301..9822)
                     /gene="prfH"
                     /locus_tag="ACHOUK_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(9821..10024)
                     /locus_tag="ACHOUK_003369"
                     /pseudo
     CDS             complement(9821..10024)
                     /locus_tag="ACHOUK_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            complement(10343..10795)
                     /gene="yafP"
                     /locus_tag="ACHOUK_003370"
     CDS             complement(10343..10795)
                     /gene="yafP"
                     /locus_tag="ACHOUK_003370"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9898753.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVMMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPWIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFTNFSMRYKPQH"
     gene            complement(10805..11203)
                     /gene="yafO"
                     /locus_tag="ACHOUK_003371"
     CDS             complement(10805..11203)
                     /gene="yafO"
                     /locus_tag="ACHOUK_003371"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HFP9898754.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDESHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            complement(11206..11499)
                     /gene="yafN"
                     /locus_tag="ACHOUK_003372"
     CDS             complement(11206..11499)
                     /gene="yafN"
                     /locus_tag="ACHOUK_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HFP9898755.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAEKYLNDMTDDDFNDFKE"
     gene            complement(11551..12606)
                     /gene="dinB"
                     /locus_tag="ACHOUK_003373"
     CDS             complement(11551..12606)
                     /gene="dinB"
                     /locus_tag="ACHOUK_003373"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFP9898756.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVSAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(12677..13462)
                     /gene="lafU"
                     /locus_tag="ACHOUK_003374"
     CDS             complement(12677..13462)
                     /gene="lafU"
                     /locus_tag="ACHOUK_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFP9898757.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            13434..15146
                     /locus_tag="ACHOUK_003375"
                     /pseudo
     CDS             13434..15146
                     /locus_tag="ACHOUK_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(15291..15788)
                     /gene="rayT"
                     /locus_tag="ACHOUK_003376"
                     /pseudo
     CDS             complement(15291..15788)
                     /gene="rayT"
                     /locus_tag="ACHOUK_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414763.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
     gene            complement(15964..16713)
                     /gene="yafL"
                     /locus_tag="ACHOUK_003377"
     CDS             complement(15964..16713)
                     /gene="yafL"
                     /locus_tag="ACHOUK_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP9898758.1"
                     /translation="MSLPSIPSFVLSGLLLICLPFSSFASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQVSYIVEGNAESRRALRQHNREQIKQHPEWFPAPLKASDRRWQA
                     LAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDKGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GETIRVSRLAEPFWQDHFLGARRILTEETIL"
BASE COUNT         4196 a         4332 c         4218 g         4020 t
ORIGIN      
        1 aagccacaca accctgcaaa aaataaatct atataaaaaa catacagata accatctgcg
       61 gtgataaatt atctctggcg atgttgacac aaataccact agcggtgata ccaagcacat
      121 cagcaggacg cactaaccac catgaaggtg atgctcttaa aaattaagcc ctgaagaagg
      181 gcagcattca aagcagaagg ctttggtgtg tgtgatacga aacgaagcat tggccggaag
      241 tgcgaatccg gattagcagc caatgtgcca ttgcggggtg ttttcgttca ggactacgac
      301 tcccacacac aaccaaagct aactaacagg agaatccaga tggatgcaca aacacgccgc
      361 cgcgaacgtc gcgcagagaa acaggctcaa tggaaagcag atcggattct ggcttcaaca
      421 tttcgcagga atgcaactaa gagacattac tgaatcaaaa atttattcag caatgcagaa
      481 aatgacgaac cggcgtcatg aggaaaactg gaaactcagg gcagaagcat gcagaaaaaa
      541 agggaaacct gttccagaat acacgccaaa accagcgtcc gttgcaacga aggctacgca
      601 tctttcattt ataaaggccc tgctaagagc cgcagagcgt gaatggaaaa tgctcgataa
      661 ggcaccaatt attaaagtgc ctcaaccaaa gaataaacgg atccgctggc tggagcccca
      721 tgaagcacaa aggctgattg atgaatgtcc ggagccatta aagtctgttg ttgaatttgc
      781 actggcaaca ggcttaagac gctcgaacat catcaacctt gaatggcaac aaatagatat
      841 gcagcgccgg gtggcatgga taaacccgga agagagtaaa tcaaaccgcg caattggcgt
      901 tgcgctgaat gatactgcat gtcgcgtatt gaaaaaacaa atcggtaatc atcaccgttg
      961 ggtatttgtg tacaaggaaa gctgtaccaa accagacgga acgaaagcgc caacagtaag
     1021 gaagatgcgg tatgacgcaa acacagcctg gaaagcggcg ctgagacggg ctggtattga
     1081 tgatttcaga tttcacgact tgagacacac ctgggcaagt tggctggttc aagccggagt
     1141 cccgttgtca gtgttacagg aaatgggagg ctgggagtct atcgaaatgg ttcgtcgata
     1201 tgctcacctt gcacctaatc accttaccga acacgcacgg caaatagact cgatcctgaa
     1261 cccatcggtc ccaaatttgt cccagtcaaa aaataaggaa ggtactaatg atgtgtaact
     1321 tattgattta aatggtgccg ataataggag tcgaacctac gaccttcgca ttacgaatgc
     1381 gctgctctac caactgagct atatcggccc tgaaaggaca tgttcacgaa cgtgaatcac
     1441 ggtggacaag gttaaaacta accgggcgat gcgtcaatgg ccttgtgaat caaacggcta
     1501 cttttgcatc acccggtttt atttacgcac gaatggtgta atcaccaatg ccgatccact
     1561 tgtaagtggt cagtgcttcc agccccattg ggccacgcgc gtggagtttt tgtgtgctta
     1621 ccgccacttc cgcacccagt ccaaactggc cgccgtcggt aaaacgcgta gaggcgttaa
     1681 cgtaaacagc ggacgaatcc acttcgttaa caaaacgctg ggcgttgcgc atatcgcggg
     1741 tcaggatcgc atcggagtgt tgcgtgccgt gttcacgaat atgggcgatg gcatcgtcaa
     1801 gatcgctgac gattttgacg ttcaaatcta atgacagaaa ctcatcgtca tactcttcgg
     1861 ctttaaccgc caccaccttc gcagggcctg tctgcaactg cgccagcgca gatgcatctg
     1921 cgtgtaacgt cacgccgctt tccgccattt gtttgcttaa tgcgggcagg aagctatagg
     1981 cgatgttttt attcaccagc aacgtttcta ccgtattaca tgtgctcgga cgctgagttt
     2041 tcgcattgac gattactttc agggcttcag caatctctgc actttcatca acataaatat
     2101 ggcatacgcc tataccacct gtgatcaccg ggatcgtcga ctgctcgcgg cacagtttat
     2161 gcaaaccagc cccgccgcgc gggatcagca tgtcgatgta tttatccata cgcagcattt
     2221 cactgaccag cgcacggtca ggattatcaa tcgcctgcac ggcacccgcc ggtaagccac
     2281 aggatttcag ggcgtcctga atcaccacca ccgttgccgc gttagtgcga caggtttctt
     2341 tgccaccacg caggatcacc gcgttaccgg ttttcaggca cagcgaagcg acatcaaccg
     2401 tcacgttcgg gcgcgcttca taaatcacgc caataacccc cagcggtacg cgacgacgct
     2461 caagacgcag gccgctgtcc agtacgccgc catcgattac ctgccccact ggatcggcga
     2521 ggttgcacac ctgacgtaca tcgtcggcaa tgcctttcag ccgtgcgggc gtcagtgcca
     2581 gacggtcaag catcgcttcg ctaaggccat tggctcgcgc gtcagcaaca tcctgggcgt
     2641 tagcgttgag gatgatttcg ctttgtgctt ccagttcatc ggcgattttt tccagcacgc
     2701 gatttttttc gcggctggag agttgcgcta atttatacga ggcttgcttc gcggcaatgc
     2761 ccatttgttc cagcatcagc ctgctcctta acgggtgatc atgtcatcac ggtgaacggc
     2821 aaccgggccg tattcatatc ccagtattgc atcaatttct tgcgagtggt gtccggcaat
     2881 acggcgtaat gcatcgctgt tgtaacgact gacgccgtgg gcgatatcgc gaccttcgag
     2941 gttacaaatg cggatgactt caccacgcga gaagttgcca gtcacgcttt taatgccttt
     3001 cggcaacagg gagctgccgc gttcaagaat ggcggcggtt gccccttcat ctaccgtgat
     3061 ttcacccgca ggcggcgcac cgaaaatcca gcgtttacgg ttttcaagcg gagtcgcctg
     3121 ggcatggaac agcgtaccga cggaaatgcc ttccatcaca tcaccaataa cgcccggctt
     3181 gctgcccgcg gcaataatgg tgtcgatacc cgcacggcaa gccacgtcag cggcctgcaa
     3241 tttggtactc atgccgccag ttccgaggcc tgaaacgctg tcaccagcaa tcgcgcgcag
     3301 tgcgtcatca atgccgtaaa catctttaat cagttctgcc tgcggattgc tgcgcgggtc
     3361 agcggtatac aaaccttttt gatcggtcag taacaacagt ttatcggcac ccgccagaat
     3421 cgccgccagt gcagaaaggt tgtcgttatc gccgacttta atctctgccg tagcgacagc
     3481 atcgttctca ttgattaccg gaacgatatt gttatcgagc aacgcacgca gggtgtcgcg
     3541 ggcgttcagg aagcgttcac ggtcttccat atcagcacga gtcagcagca tttgcccgac
     3601 gtgaatgcca taaatcgaaa acagctgttc ccacagttga atcagtcgac tctgccctac
     3661 cgccgccagc agttgtttcg aggcgatagt cgctggcagt tccgggtaac ccaggtgctc
     3721 acgtccggcg gcgatcgcgc ccgacgtcac aataacaatc cgatgcccgg cggcatgtaa
     3781 ctgcgcgcac tggcgaacaa gttcaacgat atgggcacgg ttcaggcggc gcgatccgcc
     3841 tgttagcaca ctggtgccga gttttaccac cagcgtctgg ctgtcactca tgattctctg
     3901 ccattcaatt ttaggaaaaa tgatatcaaa cgaacgtttt agcaggactg tcgtcggttg
     3961 ccaaccatct gcgagcaaag catggcgttt tgttgcgcgg gatcagcaag cctagcggca
     4021 gttgtttacg cttttattac agatttaata aattaccaca ttttaagaat atcattaatc
     4081 tgtaatatat ctttaacaat ctcaggttaa aaactttcct gttttcaacg gggctctccc
     4141 gcagaatatt cgcgcgttaa ttaaaatcag gaatgaaaat gaaaaagagc actctggcat
     4201 tagtggtgat gggcattgtg gcatctgcat ccgtacaggc cgcagaaata tataacaaag
     4261 acggtaataa actggatgtc tatggcaaag ttaaagccat gcattatatg agtgataacg
     4321 acagtaaaga tggcgaccag agttatatcc gttttggttt taaaggcgaa acacaaatta
     4381 acgatcaact gactggttat ggtcgttggg aagcggagtt tgccggaaat aaagcggaga
     4441 gtgatactgc acagcaaaaa acgcgtctcg cttttgccgg attgaagtat aaagatttgg
     4501 gttctttcga ctatggccgt aacctgggcg cgttgtatga cgtggaagcc tggaccgata
     4561 tgttcccgga atttggtggc gactcctcgg cgcagaccga caactttatg accaaacgcg
     4621 ccagcggtct ggcgacgtat cggaacaccg acttcttcgg cgttatcgat ggcctgaact
     4681 taaccctgca atatcaaggg aaaaacgaaa accgcgacgt taaaaagcaa aacggcgatg
     4741 gcttcggcac gtcattgaca tatgactttg gcggcagcga tttcgccatt agtggggcct
     4801 ataccaactc agatcgcacc aacgagcaga acctgcaaag ccgtggcaca ggcaagcgtg
     4861 cagaagcttg ggctacaggt ctgaaatacg atgccaataa tatttatctg gcaacttttt
     4921 attctgaaac acgcaaaatg acgccaataa ctggcggctt tgccaataag acacagaact
     4981 ttgaagcggt cgctcaatac cagtttgact ttggtctgcg tccatcgctg ggttatgtct
     5041 tatcgaaagg gaaagatatt gaaggtatcg gtgatgaaga tctggtcaat tatatcgatg
     5101 tcggggctac atattatttc aacaaaaata tgtcagcgtt tgttgattat aaaatcaacc
     5161 aactggatag cgataacaaa ttgaatatta ataatgatga tattgtcgcg gttggcatga
     5221 cctatcagtt ttaatgatta ttgccggatg tgatgcatcc ggcacatttc actcacgccg
     5281 ttaacttcac cggctcgtca cgaaaatcat ccgccggttc caacttcaga ttcagcgtcg
     5341 tcagcagttc acgcagcttc tcgtgaaact cacgcagggt gtgctccagt cgttcaacca
     5401 cttcagtatc ttttaccgga acactcttcc agtcgcctgc tttatcgaac agaccaaact
     5461 ggtaactgta ggtaaaacgg gattcctgcg cttcaagctc catccaccag ccccagaact
     5521 cacgcacttc cggtgccggt ttcacgttga cgcatacagc cagacaatcg aaaaagaatc
     5581 gattatcttc gcacttacct tcacgaatat acgggcctag tacggtaaat tttttgatca
     5641 atctgctctt cgggtgtcca ctcggtaacg tcattgctat ctcctgttgt gatgcaactg
     5701 ttttaccaaa ttggcaaaat ttagcaaatt tttaacacaa gcgtttttcg atccagtcgg
     5761 tgatttcctg aagacctttg tcaaaattcc gatacaccgg gttaaatggg atctctaata
     5821 atttaccgtc agcagatgat gaggtgatta agcgtgagtc ctcttccggg ctgaacggat
     5881 cgttcttcca gtagcctgat aacattggcg ttgggcatcg acgtcccagc aatccttgca
     5941 cttttaatga atagcgattc agctccacgc gcaacgcttc gtcggaagca tcatgcatcc
     6001 ccaaacgact cgccagaacg tcaagataca tttccggcac ctgttgctgg cacttaaaat
     6061 cactcaacag ggtatgaact accggaccaa gacaggcaac cgctttcaga cgcggcgatt
     6121 caagatatgc cagacgcacg gcaacgttag cgccgaaacg gaaaccaaag gccgcgacgc
     6181 gagtgtgatc cacccacggt acgttaggca gagcctttaa gacgcgctga tgcaacaggc
     6241 aggagtcctg ggtgagcttc cattttgaag aaaagcccac cgacggcata tcaatagtca
     6301 gcatcgcaat gccgcgcggc gcaaaataac gttcatacag gctgtaatag tccgtctgca
     6361 tcgcatccag accaccacac attaataccg tcgggaacgg gccatcgcct ttcggcatat
     6421 gcaaaaagcc ggtgatgggc gcaccgccgg gtacggtaaa ctccatctgt cgcatcgtgc
     6481 ccggtagacg ctgagcggcc tcttcatagg cgcggtttga caaagcctgc gcttgctcgg
     6541 ccaggtcatc tcctttcaga tgaggatagg cggcaatgtt gtacaacgta gccgcatgca
     6601 gccagtgacg accactgagt tgcggatctt cttccgcaca ggctttttgt tgccacacca
     6661 tcgcctgggt ggcccactca taaatccagt tgccgccacg gtagccaatc accgtatcgt
     6721 ataaatcatc gtcggtacgt tcggcatcgc tcatcacaat acgtgcctgg acgtcgagga
     6781 tttcgcgtgg gtcaatgccg cgccagatcc acatcagacg gttaatcatg cgataccagt
     6841 gagggatggt tttaccatca agggccgact gcacaggcgg ttgtgcgccg tgattaaagc
     6901 ggcggactag cgtcgaggtt tcaggatgtt taaagcgggg tttgaacagg gtttcgctca
     6961 ggtttgcctg tgtcatggat gcagcctcca gaatacttac tggaaactat tgtaactcgc
     7021 ctgaagttaa aaagaacaac gcccggcagt gccaggcgtt gaaaagatta gcgaccggag
     7081 attggcggga cgaatacgac gcccatatcc cacggctgtt cgatccaggt atcttgcggg
     7141 atatcaacaa cataatcatc aaccagcgga cgaccagccg gttttgcaaa gatggtgaca
     7201 aagtgcgctt ttggatacat ttcacgaatc gcaaccgcag taccaccggt atccaccagg
     7261 tcatcaataa cgatgaagcc ttcgccatcg ccttctgcgc gtttcagcac tttaagctcg
     7321 cgctggttgt cgtgatcgta gctggaaata caaacggtat cgacatgacg aatacccagt
     7381 tcacgcgcca gtaacgcacc cggtaccaga ccgccacggc ttacggcaat aatgcctttc
     7441 cattgttcag aaggcatcag tcggcttgcg agtttacgtg catggatctg caacatgtcc
     7501 caggtgacga tgtatttttc gctcatgtga agtgtcccag cctgtttatc tacggcttaa
     7561 aaagtgttcg aggggaaaat aggttgcgcg agattataga gatctggcgc actaaaaacc
     7621 agtatttcac atgagtccgc gtctttttac gcactgcctc tccctgacgc gggataaagt
     7681 ggtattctca aacatatctc gcaagcctgt cttgtgttga caacattttc tgctaaccct
     7741 gtgacctgca atactgtttt gcgggtgatc gacaaggaga cttaacgtgt ctgaactgtc
     7801 tcaattatct ccacagccgc tgtgggatat ttttgccaaa atctgttcta ttcctcaccc
     7861 gtcctatcat gaagagcaac tcgctgaata cattgttggt tgggcaaaag agaaaggttt
     7921 ccatgtcgaa cgcgatcagg taggtaatat cctgattcgt aaacctgcta ccgcaggtat
     7981 ggaaaatcgt aaaccggtcg tcttgcaggc ccacctcgat atggtgccgc agaaaaataa
     8041 cgacaccgtg catgacttca cgaaagatcc tatccagcca tatattgatg gcgaatgggt
     8101 taaagcgcgc ggcaccacgc tgggtgcgga taacggcatt ggtatggcct ctgcactggc
     8161 ggttctggct gacgaaaacg tggttcacgg cccgctggaa gtgctgctga ccatgaccga
     8221 agaagccggt atggacggtg cgttcggctt acagagcaac tggttgcagg ctgatattct
     8281 gattaacacc gactccgaag aagaaggtga aatctacatg ggttgcgcgg ggggtatcga
     8341 cttcacctcc aacctgcatt tagatcgtga agcggttcca gctggttttg aaaccttcaa
     8401 gttaacctta aaaggtctga aaggcggtca ctccggcggg gaaatccacg ttggcctggg
     8461 taatgccaac aaactgctgg tgcgcttcct ggcgggtcat gcagaagaac tggacctgcg
     8521 tcttattgat ttcaacggcg gcacactgcg taacgccatc ccgcgtgaag cctttgcgac
     8581 cattgctgtc gcagctgata aagtcgacgc cctgaaatct ctggtgaata cctatcagga
     8641 gatcctgaaa aacgagctgg cagagaaaga gaaaaatctg gccttgttgc tggactctgt
     8701 agcgaacgat aaagctgccc tgattgcgaa atctcgcgat acctttattc gtctgctgaa
     8761 cgccaccccg aacggtgtga tccgcaactc cgacgtggca aaaggtgtgg tcgaaacctc
     8821 cctgaacgtc ggtgtggtga ccatgactga caataacgta gaaattcact gtctgatccg
     8881 ttctctgatc gacagcggta aagactacgt ggtgagcatg ctggattcgc tgggtaaact
     8941 ggctggcgcg aaaaccgaag cgaaaggcgc atatcctggc tggcagccgg acgctaattc
     9001 tccggtgatg catctggtac gtgaaaccta tcagcgcctg ttcaacaaga cgccgaacat
     9061 ccagattatc cacgcgggcc tggaatgtgg tctgttcaaa aaaccgtatc cggaaatgga
     9121 catggtttct atcgggccaa caatcaccgg tccacactct ccggatgagc aagttcacat
     9181 cgaaagcgta ggtcattact ggacactgct gactgaactg ctgaaagaaa ttccggcgaa
     9241 gtaattattt gatttgctgc cggatggcgt ttaatcgcct tccggcagtt tcatccttca
     9301 ttatccttcg ataaaagcca tccctgtgaa cgttcgtcgc gggttgccac gttcaatctg
     9361 gtgatggaac attcgccgct gcgatttcag cactgcgcta ttttcctgtt gctgctgctc
     9421 cagcttccag gcaatcagca atcgcgccag ccgtttgtta gcatgctggc tgcgttctga
     9481 ctgaaccttc acgctaatcc cggttgccag atgcgtggcg cgtaccgccg agtcggtttt
     9541 attgacatgt tgaccgcccg gccccgacgg acgcagcgtc tcataacgga ttgcatccga
     9601 ttgttcctgc tcatcagcgg taaaacgccc aatgcccaga aaccagtttt tgcgcccatg
     9661 atgaggccga tacggactca gacaaatcca ctgaatagtg ccgcaccaac tttcgcttaa
     9721 cgcccatgcg ttgtcgccat ccagagaaat cagcgccgaa cgcagcgtgt cagagtagcg
     9781 gcccgtttct gtttccagca ccgttaccgc gacgtcttgt cgggtaaaaa cgaccaacag
     9841 agaagaacgc tgcgccaatc gggtagccgc gtccggggtg taaatctggc atccccacta
     9901 cgcgctgcat gttgggtaaa tttgccgtgg tgtgtaattg ttggatcgct aaactctcga
     9961 tccacaggtc atcagatgcg atggtaaata ccgcatcgga taagggacga atatatttgc
    10021 ccatgtacca ttccatttat actcgtcata cttcaagttg catgtactgc gactgcgttc
    10081 gttcacccca gtcacttact tatgtaagct cctggggatt cactcgcttg tcgccttcct
    10141 gcaactcgaa ttatttagag tatataaata taaaaatcag gaaatggcag acgaatccag
    10201 ggcaagaaaa aaaagaatta atcctggaaa ggtctgcaaa gagtcattga taaaggattt
    10261 gtcatgattt acctccttgc agtttgtgga ataagaaaac gcgcacatac caggcgggcg
    10321 ttattttcat tgcaagctgg atttaatgtt gcggtttata tcgcatagaa aaattagtaa
    10381 accacgctcc ccggcattca acgcgctgct gcttaactgt ctgaaaacca taacgttcaa
    10441 aaaagggttt tgcggttatg ctggcgtcca ccgtaagttc ggattcagac ttaatccaag
    10501 gttttaacaa agcgctggca accccacggc gggtgtattc agggtcaaca aataacatat
    10561 cgatataatg ttcaatgcgg gaaataaaac caaccggttg tgcattaatg actgcaaccc
    10621 gcacttgtga tttcgcgagt ttctccttcc agcgagattc gtcaatctgc gcccaggcgg
    10681 caatttgttg tggtgaataa tgctgactgg cggtcatcat aaccgctcta atgaaaatag
    10741 cgcatagttg ctgaaaatcg ccaggctgat agtttcttat ttgtatgtta ttcataatat
    10801 aaattcaaaa acgcatgcga aacgcttctg ccattttccc aattttatgc atttggttgt
    10861 tatctcgagc cagtttatga ggttcaggtt tcagaatggc aatgagcaac catgcttgct
    10921 catcaaacgc accctgacaa tataccaaat gcgattcgtc attcgttctg ctgaattggc
    10981 gcaactgtgg cggaaatgga ttattcacat ttgccagatg aatatgagca actcgctcaa
    11041 atttgattaa tggccaagta aacgagtcgt cgtagagtgc atcgcgacca aatatatctg
    11101 gcaaaacacc gtcacgctta taggaaataa aatccgccgt taacgcatca agctcctctg
    11161 ctgtaagttg caggcgaata agttttgttt tgaatacccg catccttatt ccttaaagtc
    11221 attgaaatca tcatccgtca tatcattaag atatttttca gctcggcgtg taatttcctc
    11281 taatcttgca gcacttggac ggaagcgcgc ctttatgggc tttttctcct cttgttcagc
    11341 aagcatgtcc attaacgctt cgaatgcgct ggcacttaag agatatcctg cggggcgatt
    11401 attagaaaga accgcaaccg gttgatcaat aaagtattta gctgggtttt tacgtaattc
    11461 agtgatattg accgattttt cagcgagaat tcgatgcata cagtgatccc ctcataataa
    11521 tgcacaccag aatatacata atagtataca tcataatccc agcaccagtt gtctttccat
    11581 ttgagggtca agcaacgtca catgcagccc caccagtcgc acaccgcgcc cgccgcggcg
    11641 ttcatcccag gttttacgcg cggtggcgat taagtcagct ttattcagcc gcggccagac
    11701 gtgctcctgg gtggtttgct gaaaatcgtc gaactttaat ttcaccccct ggcgagcaat
    11761 cagtaaatca ggttttacct ttgccagacg gcgttcaagt tccggataca gccgctcgat
    11821 aatcgcttca cattcagacc agtgatgaat atcttccgcc atcgtgcgtt ccacgccgac
    11881 ggattttcgt aaccgttcgc tgttaacgtc gcgctcgtca atcccctgac tacgctccca
    11941 caaaatgcgg ccaaatttgc caaagcgttt aagcagaatc accagatcac acttttgtac
    12001 atcaccgcag gtccgcagcc ccatcgcttc cagctttgcc gctgagactt tgccaacacc
    12061 gggaattttt gccagcggta aggtttgcag aaatgccgaa acttctgccg gcgtaatcac
    12121 aaactggccg ttgggtttat tcatgtcgga ggcgattttg gcgagaaact ttactggtgc
    12181 cacgcccgca gacgccgtca gttgcagctc gctgaaaatt gtctggcgga tttcctgggc
    12241 gatgagggtc gcagaaccgt ggcaatggac gctatcagtg acatcgaggt aggcttcatc
    12301 cagtgacaac ggttcaatac gcgaggtgta gcgcgagaag atttcacgga tatgatttga
    12361 ggcttctttg taggcgtcaa agcgccccgg aagcaaggtg agatgtgggc ataatttgag
    12421 cgccatccct gtcggcatag cgctgcgtac gccaaattta cgcgcgggat aattggcggt
    12481 gctgatcacc ccccgacgtt cgcggctgcc gccaatagca atagggatat cgcgcagggc
    12541 gggattgtcg cgcatctcca ctgcggcgaa aaagcagtcc atatccacat gaatgatttt
    12601 acgcattact cacctctcaa cactggtaaa gtatacagtg atttcagggt ttgagaaatg
    12661 cgtaaagatt cagcatttag cgcgttcgct gcgaaagcac ctgctgctta tccagctttt
    12721 gcaccagcgg ctgcaccact ttatccccat gctgaccaaa gtattgatac agcgtatcgg
    12781 acgcactttt ggtcagcacc ataatctcaa tgcgtcggtt gcccgcgctt tgcggatttt
    12841 tcgcgtccag cagcatctgg tccgccattg cgcttacctg catcacttta tcttccggca
    12901 ttccggcctc ttccagcacc cgacgagctg aaagcgcgcg atcgcccgaa aggttccagt
    12961 tgttgtagat attgtttttg taggccatcg catcggtatg cccggtaata ataattttgt
    13021 tatcgagcga gtcgaacact ggcgcgagct ccaccagcaa agttttaaag aacggcataa
    13081 tctgcgcgct gccgcgttcg aacatattgc gattctggtc gtctttaatc agcacgcgta
    13141 atccctgcgg gacaatctcc atctccagat tcgcttccat atgcgcatca cgggcaatgg
    13201 tgttgatact ggttgccagc tcgccaagct cggtggcgga tttcttcttc agcagcgccg
    13261 ttttctcatt cacatcacgc gcttttttct ccgtctcttc cggcaccgcc acggtggcag
    13321 gttttggcgg atgagacggg ctgattttat ttaacggcga cagcccacca ccgttaaaaa
    13381 tcgactgccc gtgcagcgcg gcgataatgc tttcacgctc ggatttactg acgctgttga
    13441 caatcaactt tatcgtcgtc accaaagggg ccgagcgtat ttccgaggtt tctgcccgct
    13501 ttaccctgga tgcgatgccc ggcaaacaga tggcgattga cgccgatctt aacgccggat
    13561 tgatcaacca ggcacaggcg caaacccggc gtaaagatgt tgccagcgag gccgatttct
    13621 acggcgcgat ggacggggca tcgaagtttg tgcgcgggga tgccatcgcc gggatgatga
    13681 ttctggcgat taacctgatc ggcggcgtct gtatcgggat atttaaatac aacctgagcg
    13741 ccgacgccgc cttccagcaa tatgtgctga tgaccatcgg cgatgggctg gtggcgcaga
    13801 tcccttccct cctgctctcc accgcagcgg cgattatcgt cacccgcgtc agcgacaacg
    13861 gcgatatcgc ccatgacgtg cgccaccagc tgctggcaag cccgtcggtg ctctacaccg
    13921 ccaccgggat tatgtttgtg ctggcggtgg tgccgggaat gccgcacctg ccgtttttgc
    13981 tgttcagcgc cctgctgggt tttaccggct ggcggatgag caaacagccg caggcggcgg
    14041 aggcggaaga gaaaagcctc gaaacgctga cccgcaccat cactgaaacc agcgagcaac
    14101 aggtcagttg ggaaaccatt ccgcttatcg agcctatcag tttaagcctc ggctacaagc
    14161 tggtggcgct ggtcgataaa gcgcagggca acccgctcac ccagcgtatt cgcggcgtac
    14221 ggcaggtgat atccgacggt aacggcgtgc tgctgccgga gatccgcatt cgggaaaact
    14281 tccgcctcaa gcccagccag tacgccattt tcatcaacgg cattaaggcc gatgaagcgg
    14341 atattccggc ggataaactg atggcgctgc cctccagcga aacctacggc gagattgacg
    14401 gcgtgctggg gaacgacccg gcgtacggga tgccggtgac ctggatccag cccgcgcaga
    14461 aggcgaaggc gctgaatatg gggtatcagg tgatcgacag cgccagcgtg attgccacgc
    14521 atgtgaacaa gattgtgcgc agctatattc ctgatttgtt taattatgat gacatcacgc
    14581 agctgcataa ccgcctgtcg tcgatggccc cgcgcctggc ggaagatttg agcgcggcgc
    14641 tgaattacag ccagttgctg aaagtgtacc gtgcgctgct gaccgaaggc gtttccctgc
    14701 gcgatatcgt caccatcgcc accgtgctgg tcgccagtag cgcggtgact aaagatcata
    14761 ttctgctggc ggccgatgtg cgcctggcgc tgcggcgcag cattacccat ccgttcgttc
    14821 gcaagcagga gctgacggtg tatacgctga ataatgagct ggaaaatctg ctgactaacg
    14881 tagtgaatca ggcgcaacag ggcggtaaag tgatgctcga cagcgtgcca gtggacccga
    14941 atatgctcaa ccagttccag agcacgatgc cgcaggtgaa agagcagatg aaagcggcgg
    15001 ggaaagaccc ggtgctgctg gtgccgccgc agctgcgccc tttgctggcg cgctatgcaa
    15061 ggttgtttgc gccggggctg catgtgctgt cgtataacga agtgccggat gagctggagt
    15121 tgaagataat gggggcgttg agttgattgg cctttaaggg gcgcatccgg cattggtgca
    15181 caattgccgg atgcggcgtg aacgccttat ccggcctacg gttgagtgca gttttgtagg
    15241 catgataaga cgcgccagcg tcgcatcagg catccggcac cagcgcacaa ttatgaaatg
    15301 atacgctccc cggcactaaa atccgttatg tctcccgccc aatcaatggg atataaccct
    15361 cgcgccacat cgcgatggaa cgttgagaat ggccaatcac tcacttgctt tacccagcca
    15421 tgttttactg gatttatata aatataatca acatgatggc gatagtcttt ggtattgcgg
    15481 atggtgtgct cctaaaaacg tggttgccag atatttttca atccacaagc atgggtgaac
    15541 tgctttttaa tctcccgcca gcgggaggaa aaatcatcat cgctttcggg taatgtccat
    15601 atgcaatgca tatgctccgg caaaacgacc caggcgttaa tttcaaaagg tctgtctcgc
    15661 ttaacgttaa taatggcgtt acggagcatc tggaactgag cggtcagaag gtggcttcga
    15721 cgatttcgta ggttaaccgt gaaaaaccag gttccccctt tgatgtaata ccgacgatat
    15781 tcagacatgg caactccttt ttctcaacgg taagaagaaa aggaaaggtc ggcaatgagg
    15841 tgatggaata acgggaaagc ggctcgcaaa aacgcacaga tttgtaggca tgataagacg
    15901 cgtcagcgtc gtatcaggca tctgcgcaca actgccggat gcggcgtgaa tgccttatcc
    15961 gtcctacaaa atcgtctctt ccgtcaaaat ccggcgcgcg cctaaaaagt gatcctgcca
    16021 gaaaggttca gctaaccgac ttacccgaat ggtttccccg gtgcgcggtg actcaataaa
    16081 ctgaccgtcg cccagataca cccccatatg gtcggcaatc tcgcggctgt ggatatggaa
    16141 aaacagcaaa tctccccggc gcaggtcgtt attcgccaca atcgttgccc gatggtagtg
    16201 atacatctca ttagccgtac gcgggagctt cgcctcgagg atcttgttgt aggcataaaa
    16261 aaccaacccg ctacagtcaa agcctttatc aggccgcgtg ccgccccaga cgtaaggctt
    16321 gccaagctgc tgctccaggc ggtgaatcgc cacttcggta atgttatgca gatggtcgct
    16381 gcttaaaaag tggttgtttt ccgccagcgc ctgccagcgt ctgtcactcg ccttgagcgg
    16441 tgcaggaaac cattctggat gctgttttat ctgttcgcgg ttgtgctggc gtagcgccct
    16501 tctgctttcg gcattgcctt ccacaatata gctgacctgc tttttcagat gggtttggta
    16561 ctgttttaat aaacgcgcac ggttttgcat ccgctggcgg gcggcgtagc tgaaagagat
    16621 atgtgatgtg gtggcgctgg caaatgaaga aaacggcaaa caaatcaaca gtaatcccga
    16681 caatacaaag gatggaatcg acggcaagga catactcaat aatggctggt catgcattga
    16741 tgttacagtg tatcccggcc cgtacc
//
LOCUS       DAZKIP010000094        16631 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZKIP010000094 DAZKIP010000000
VERSION     DAZKIP010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.095"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..182
                     /locus_tag="ACHOUK_003378"
     CDS             <1..182
                     /locus_tag="ACHOUK_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFP9898759.1"
                     /translation="DKQYVWCWGKSYGWTECDTFEGYYDCSRNKWWAVTDNGEEPASK
                     VTHWMPLPEPPQEVK"
     gene            270..512
                     /locus_tag="ACHOUK_003379"
     CDS             270..512
                     /locus_tag="ACHOUK_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP9898760.1"
                     /translation="MFALIQRGQIYTDRAGYPVVITRSTQHSVFFRRMDGRSGRVRIG
                     EFNNLFEHIDQQEYRKILAGTEQEMRLKKLRAMQRR"
     gene            516..650
                     /locus_tag="ACHOUK_003380"
     CDS             516..650
                     /locus_tag="ACHOUK_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9898761.1"
                     /translation="MHTAFEFWVRKTFGNRYDLTRDVDGFYCREVVKRMFDVWCHCRG
                     "
     gene            669..923
                     /gene="xisR"
                     /locus_tag="ACHOUK_003381"
     CDS             669..923
                     /gene="xisR"
                     /locus_tag="ACHOUK_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFP9898762.1"
                     /translation="MQTIIYQITPSKWCTERVLIASTGLKPGTIERARRKSWMQGKEY
                     RHYAVEGDPGHYSECLYNIEEIMRWIENQKQPGAKNASSG"
     gene            957..2243
                     /locus_tag="ACHOUK_003382"
     CDS             957..2243
                     /locus_tag="ACHOUK_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9898763.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKREITVKALSEKWLDLKKIEICAN
                     ALNRYQSVIKNMLPMLGEKKLVSSITKEDLLFVRRDLLTGYQKLSNGKTSSIKGRSVV
                     TVNYYMTTIAGMFQFATDNGYTSGNPFNGLAPLKKSKVKPDPLTRDEFIRFIEACRHQ
                     QTKNLWILAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGRT
                     IHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRTAVHKCTFVFSPQVTKQQQLSGP
                     HYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMV
                     FNVYGAWMKDNNHEQIELLNKRLSESVPCMPHKKAG"
     gene            2264..2965
                     /gene="mlrA"
                     /locus_tag="ACHOUK_003383"
     CDS             2264..2965
                     /gene="mlrA"
                     /locus_tag="ACHOUK_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFP9898764.1"
                     /translation="MSLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYP
                     AQTLTTHLFIPLRRRLQYQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGW
                     NIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQL
                     TSWQEQGHDIFPLGI"
     gene            3025..3132
                     /gene="yohO"
                     /locus_tag="ACHOUK_003384"
     CDS             3025..3132
                     /gene="yohO"
                     /locus_tag="ACHOUK_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFP9898765.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(3113..3844)
                     /gene="yehW"
                     /locus_tag="ACHOUK_003385"
     CDS             complement(3113..3844)
                     /gene="yehW"
                     /locus_tag="ACHOUK_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFP9898766.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(3849..4775)
                     /gene="yehX"
                     /locus_tag="ACHOUK_003386"
     CDS             complement(3849..4775)
                     /gene="yehX"
                     /locus_tag="ACHOUK_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFP9898767.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGELRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPSGTLHFQDLLVEA"
     gene            complement(4768..5925)
                     /gene="yehY"
                     /locus_tag="ACHOUK_003387"
     CDS             complement(4768..5925)
                     /gene="yehY"
                     /locus_tag="ACHOUK_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFP9898768.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLASALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAGLVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            complement(5932..6849)
                     /gene="osmF"
                     /locus_tag="ACHOUK_003388"
     CDS             complement(5932..6849)
                     /gene="osmF"
                     /locus_tag="ACHOUK_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFP9898769.1"
                     /translation="MPLSKVWAGSLVLLAAVSLPLHAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLRRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGSDGPVAAQGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(7101..9398)
                     /gene="bglX"
                     /locus_tag="ACHOUK_003389"
     CDS             complement(7101..9398)
                     /gene="bglX"
                     /locus_tag="ACHOUK_003389"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFP9898770.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITILQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            9594..11309
                     /gene="dld"
                     /locus_tag="ACHOUK_003390"
     CDS             9594..11309
                     /gene="dld"
                     /locus_tag="ACHOUK_003390"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFP9898771.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGHG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(11347..12279)
                     /gene="pbpG"
                     /locus_tag="ACHOUK_003391"
     CDS             complement(11347..12279)
                     /gene="pbpG"
                     /locus_tag="ACHOUK_003391"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFP9898772.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(12453..13040)
                     /gene="yohC"
                     /locus_tag="ACHOUK_003392"
     CDS             complement(12453..13040)
                     /gene="yohC"
                     /locus_tag="ACHOUK_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFP9898773.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            13210..13788
                     /gene="yohD"
                     /locus_tag="ACHOUK_003393"
     CDS             13210..13788
                     /gene="yohD"
                     /locus_tag="ACHOUK_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFP9898774.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGLAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(13918..14679)
                     /gene="yohF"
                     /locus_tag="ACHOUK_003394"
     CDS             complement(13918..14679)
                     /gene="yohF"
                     /locus_tag="ACHOUK_003394"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9898775.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGNLPEGAQALEKLIQRLGRIDVLVNNAGAMTKVPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(14732..16168)
                     /gene="mdtQ"
                     /locus_tag="ACHOUK_003395"
     CDS             complement(14732..16168)
                     /gene="mdtQ"
                     /locus_tag="ACHOUK_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFP9898776.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLIALTNHQTKSLTLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            16392..16475
                     /gene="yohP"
                     /locus_tag="ACHOUK_003396"
     CDS             16392..16475
                     /gene="yohP"
                     /locus_tag="ACHOUK_003396"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFP9898777.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         4104 a         4461 c         4196 g         3870 t
ORIGIN      
        1 acgataaaca gtatgtttgg tgttggggga agtcttacgg ctggactgag tgcgatacct
       61 tcgaagggta ttacgattgt tcgagaaaca aatggtgggc agttactgac aatggggaag
      121 aaccggcatc gaaagtaacc cactggatgc cgctaccgga gccgccgcag gaggtgaaat
      181 gatgaattgg cctgaagcat tcaccgcagt tggagttgca atagcggtgg catttattct
      241 gtattcgctt ttccgctggg gataaaggaa tgttcgctct gattcaacgt ggtcagatat
      301 acacggacag agccggatac cctgtggtga ttactcgcag tactcagcac tcagtgttct
      361 ttcgacgcat ggacgggcgc tccggacggg tacgcattgg tgagttcaac aacctgttcg
      421 aacatattga ccaacaggag taccgcaaaa ttctggcggg cactgagcag gaaatgcgcc
      481 tgaaaaaatt acgcgcaatg caacggaggt gatacatgca tacggctttt gagttctggg
      541 ttcgcaagac attcggcaat cgctacgacc tgacccgtga tgtcgacggc ttctactgcc
      601 gtgaagttgt gaaacgaatg tttgacgtgt ggtgccactg ccgtggatga aagttttatg
      661 aggttggcat gcagacaatc atctatcaga taacccccag caaatggtgt acggagagag
      721 tccttattgc atcaacaggg ctaaagcccg gcaccatcga gcgggccaga agaaagtcat
      781 ggatgcaggg aaaagaatac cgccattacg ctgtagaagg tgatcctggg cattacagtg
      841 aatgcctgta caacatcgaa gaaattatgc gatggatcga aaaccagaaa caaccaggtg
      901 ccaaaaatgc aagttccggt taacctgtta atgctcctgg acgtctggga ggtttaatga
      961 gtaacgcatc atacccgaca ggcgttgaaa accatggcgg atcactccgt atatggtttc
     1021 actataatgg caaacgtgtc agagaaaacc tcggtgttcc tgacacagcc aaaaaccgga
     1081 agatcgctgg tgaacttcgc acttccgttt gttttgcaat cagaatgggg agtttcgact
     1141 acgccgcgca gttccctaat tcccctaacc tgaaacactt tggtctggga aaaagagaga
     1201 taaccgttaa ggcactttcg gaaaaatggt tggaccttaa gaaaattgag atttgtgcga
     1261 atgcacttaa ccgttaccag tcagtaatta aaaacatgtt accaatgtta ggtgaaaaaa
     1321 aactggtttc atccataaca aaagaggatt tacttttcgt aaggagagat ttgttgaccg
     1381 gttaccaaaa gctttctaat ggaaagactt cttccataaa agggcgctca gtggtcacgg
     1441 taaactacta tatgacaacc atagctggaa tgtttcaatt tgcaacagat aatggttata
     1501 cctcaggaaa cccatttaac ggtctggctc ccttaaaaaa gtccaaggta aaaccagatc
     1561 ctctcacccg tgacgaattt attcgtttta ttgaggcttg ccgtcatcaa caaacaaaaa
     1621 acctgtggat tctcgctgta tacacgggta ttcgtcacgg ggagttggta tcgctggcat
     1681 gggaagatat agaccttaaa gcaaggacta taaccatccg tagaaattat acaaaacttg
     1741 gcgaattcac tccaccaaaa accgatgcag gcaccggaag gacaattcat ctggttcaac
     1801 cagctattga tgctcttaaa agccaggcgg aaatgaccat gcttggaaag caacattctg
     1861 tagaggtgaa gcagagggaa tatgggagaa ctgctgtgca taaatgcact tttgttttta
     1921 gtcctcaggt aacaaaacag cagcagttgt ccggacctca ctacaaggtt gactccatca
     1981 gggagtcatg gacaagtatc ttaaaacgcg caggtctgag acacagaaaa tcgtaccaat
     2041 ccaggcatac ttatgcatgc tggtcacttg ccgcaggagc taatcctagt tttatcgcaa
     2101 gccagatggg ccacacaaac gcacaaatgg tattcaatgt ttacggagca tggatgaaag
     2161 acaacaatca cgaacagata gaactcctta acaaaagact atctgaaagt gtcccatgta
     2221 tgccccataa gaaagcaggg taaaataaaa acttgcaaaa tcaattagtt tacccttaat
     2281 ccctgtcacg ttacgcgcgt ggcagaggcg ttacggattg ctgaaaccgc aacggacaga
     2341 cggcggtcat cggctgttca acgatgccga tattgaccgg atccgcgaga tcaaacgctg
     2401 gatcgacaac ggcgtgcagg tcagcaaagt taaaatgctg ctcagtaatg aaaatgttga
     2461 tgtgcagaac ggctggcgcg atcagcaaga aacattactg acctacctgc aaagcggcaa
     2521 tctgcatagc ctgcgaacgt ggatcaaaga gcgcggtcag gattaccccg cccagacgct
     2581 caccacacat ctgtttattc ctctgcgccg acggcttcag taccaacaac cgactctcca
     2641 ggcgctgctg gcgatcctcg acggcgtact gatcaactac atcgccattt gtctggcttc
     2701 ggcacgtaaa aaacagggta aagatgcgct ggtggttggc tggaatattc aggataccac
     2761 ccgtctgtgg ctggagggct ggattgccag tcaacaagga tggcgcattg atgtcctcgc
     2821 ccactcgctc aatcaactac gccctgaact attcgaaggc cgtacattgc tggtgtggtg
     2881 cggtgaaaat cgaacctccg cccaacagca gcaactcacc agttggcaag aacaaggcca
     2941 tgatattttc ccactcggca tttaatgatt cgttaacaaa tgcgctttac tgtacaatcc
     3001 tttcgttaac ataaggagtg cattatgcgc atagctaaaa ttggggtcat cgccctgttc
     3061 ctgtttatgg cgttaggcgg aattggtggc gtcatgctcg caggttatac ctttattttg
     3121 cgtgctggct aagcgcctgc accagccttt caaacaggcg gtctgcgatg atcgccgcca
     3181 gtgccaccag taacgccccc tggatcacat acgcggtatt aaatccgcta agcccgatga
     3241 tgatgggcgt acccagcgtg ctggccccta ccgttgaggc gatcgtcgcc gtaccaatgt
     3301 tgataatcac cgaggttcgc acgcccgcca gaatcaccgg agccgccagc ggtagctcga
     3361 ccttacgcag tcgctgacct cgactcattc ccataccttt cgcaacttct gtcacgctgg
     3421 catcaatcgc tcccagcccg gcaagtgtcg cctgcaggac gggcagcaca ccataaagga
     3481 tcaaggcgat aatcgctggt tgcagaccaa agcctatcac cggaacagca atcgccagca
     3541 ctgcgacagg cggaaaagtc tgcccaacgg cggcaatagt ttccaccagt gggcgaaatt
     3601 ccgcgcccca cgggcgagtg actgcaattc cggcaccagt gccaatgatc accgcaaaca
     3661 aactcgaaat tcccaccagc cagaaatgag ccagtgccaa agctgcaaaa ctttcttgct
     3721 gataaacggg tcgtggcagt tgtgggaaca aggcagcaaa cagcggctgg ctgtaaggca
     3781 gccagaaaat cagtgccaca aacaaagcaa tgagccagaa cagcggatcg cgcaacatct
     3841 tcatacgctt acgcctccac cagcagatcc tgaaaatgca gcgtgccgct aggctggccc
     3901 tgcgtgttca ccaccggcag cacctcgcat ccccgcgcga caaacaggga gagcgcatcg
     3961 cgcagcgtca tctcttctgc cagtgcctca ccttctgccc gttcttcgcg acgcacgtaa
     4021 tccgccacac tacgtaacga aagcaggcgc acacccagtt cactacgtcc aaaaaactgg
     4081 cggacaaaat cattcgccgg acgagtcagc atcgtcagcg gattcccctg ctgcaccact
     4141 tcaccgtgat ccatcaatac cagatgttct gccagccgta gcgcctcatc aatatcatgc
     4201 gtgaccagca caatggtacg ccccagcaaa cggtgaatgc gcgtcatctc ttgttgcaac
     4261 gcgccgcgcg ttaccgggtc cagtgcgcca aaaggttcat ccatcagtaa gacttgcgga
     4321 tcggcagcca gtgcgcgcgc tactcccaca cgttgctgct gaccaccgga aagctgatgt
     4381 ggataacgct cacgtaaatt tgactccagc cccagtagcg ccattaattc gtcgatacga
     4441 tcgtcaatcc tcgcccgcga ccatttttgt aattgcggca cggtggcaat gttttgtgcc
     4501 acgctccagt ggggaaacag gccaatagat tgaatggcat agcccatacg gcggcgcaac
     4561 tccagtactg gcagcgagcg aatttcttct ccagcaaagc ggagctctcc gctgtcatgt
     4621 tccaccaggc ggttaatcat tttcagggtg gtggatttgc cggagccaga tgtgccaatc
     4681 agcaccgaaa aactcccttc ctgaaaattg agattgagat cgttaacggc tttttgtgcg
     4741 ccgaacagtt tgctgacatg gctaaattca atcattacgt ttcaccttca acagtgcgat
     4801 tagtaaatcg aacagcgcat cgatcagcac cgccagaaca attaccggga tcacccccag
     4861 caacactaaa tcaatggcgc tgcttagcag cccctggaaa accagcgcac caaaaccgcc
     4921 tgcgccgatt aacgccgcaa tcaccgccat gcctacagtt tgcaccatca ccacccgcag
     4981 gctgcgcaga aataccggta acgccagcgg taactgaaca tgcaggaatc gctgcgcccc
     5041 gctcatgccc atcgctctgg cgctctccag cacatcgcgc gggatctggt tcaagccgac
     5101 taccacgccg cgcaccagcg gtagcaaggc atagagcacc agcgcaatca gtgcgggtgt
     5161 cattccggtt cctgctatgc caagcttccc cagccacgga aaggccgtaa ccagtccggc
     5221 aagcggcgca atcagcaggc caaagagcgc cacggaaggc acggtctgaa tgacattgag
     5281 cagagaaaaa atcgccccct gccgcgcggt ggaaaagtag caccagatgc ccaacggcac
     5341 accaatcact aacgcaggca gcaccgcacc aaacagcaac gtcagatgtt gtgccagcgc
     5401 gtcgtcaaac acatcctgac ggttggcgta ttctttcatt agtgagagat cgttaagcgt
     5461 gccggagtac agcaaccaca gcggaataat ggcaatctgc atatgcaaca accagcgcca
     5521 cagcggatgc gtggagattc ggcggatggc atcgctacag gccagcaatg ccagcgccga
     5581 agccaaccag aaaccactgc cgaggctggt acgcgccagc gcactgccat tttgcgccag
     5641 ttgggtcgcc gcctttccag ctccccacac cagcaatacg aagacgaatt gcgccagaat
     5701 gagtgcaaaa atgctgcctt ttttagcggg aataaaacag gctgtcagcc aggcgcaacc
     5761 aacgcccacc agcatccaga tcgtttgcgg ccacagttgc cagagatgac gcccttcacc
     5821 cgagactaaa cgattaggcg cgtagctgat aaacggcagc gctgctgcga ttgccgtcag
     5881 caacagcagc agcgccagaa caggattaat acgtagataa gtcacgggaa attacttcgt
     5941 ccaccctttt tgtttcaagt agtcggcagc cacttttttg gcatccagtc cttccactgc
     6001 aatgctggca ttcagttgct gcaatgtttt tgcatcgagg ctggcgaaga ctggctgtag
     6061 ccactgtgcc atttgcggat actctttcag caccgactca cgcaccactg gtgcaggcgc
     6121 gtagataggt tgcacgcctt gcggatcgct taaggtttgc agcccctgcg ccgcgaccgg
     6181 accgtcagag ccgtaagcca ttgcggcatt aacgccagaa gtttgctggg cagcggcttt
     6241 aatcgttacc gccgtgtcgc cgccagccag tgacagtaac tgatcctgat cgagtttaaa
     6301 gtcgtaggct ttttcaaacg cgggtaacgc atcggcgcgt tcgataaact cagccgaggc
     6361 tgccagtttg aaggtgccgc cctctttcag ataacgcctc aggtcagcaa gcgaagtgag
     6421 tttgtttttc tctgccacat cctgacgcac ggcgatggtc caggtgttat ttgcaggtgc
     6481 gggcgtcagc cagattaact tgttttgctc tgcatcgagt tttttgactt tctcgtaacc
     6541 ttgctgcgcg tttttccacg ctgcatcgtt ttcatcttta aagaaaaacg cgccattgcc
     6601 ggtatattcc ggatagatat ccagttcacc ggaagtaatc gccccgcgca ccacaggagt
     6661 cgttccaagt tgcactttat tgaccgttgg tactccgtgg ctttcgagta cttgcaaaat
     6721 gatattgccg agcagcgcgc cttcggtatc gatttttgaa ccgactttaa cgggggaagc
     6781 cgcgtgcagc ggcaggctca cggctgccaa caaaaccagt gaacctgccc agacctttga
     6841 gagtggcatg atgctttcct cattctttta ctgttgtttt cagcgaatta agagaaaagc
     6901 atagttgata atggcgggat tagcctgagc ggacggattc agttgcagaa tcagataaat
     6961 acttaagagg catattcggt ccggcgtttc ctgccggatg cggcgcgagc gccttaaccg
     7021 gcctacaaag ggcgaaaatt cagcaagtta agatatgaac atcaggcaaa gggcgcaaac
     7081 gtcgcgccct tgctaaagca ttacagcaac tcaaactcgc ctttcttaac gcgtgcggaa
     7141 tcagtgccga taaagacatt gaacttgcca ggctcggcgt catatttcat ctgttgattc
     7201 cagaacttca gcgcctcgat atcaatcggg aagctgacgg tctgggtttc gcccggcttc
     7261 agggtgattt tctcaaagcc tttcagctgc ttcactggac ggctcatgga agccgtcaca
     7321 tcctgcaagt acatctgcac taccgtggcc ccttcgcgtt taccggtgtt cgtcacctgc
     7381 acgctggccg tcactttgcc gtcacgcttc atggtcggcg cagaaagttt cacatcagag
     7441 acggtgaaag tggtatagct cagaccatag ccgaacggat aaagcgcccc gttagcttca
     7501 tcaaaataac gcgaagtgta tttgttcggc ttgtcggcat tatacggacg accggtgttc
     7561 agatggctgt agtacaccgg gatctgcccg acagaacgcg ggaaggacat cggcagctta
     7621 ccggacgggt tgtaatcgcc aaacaacaca tcggcaattg cattaccgcc ttcagtcccg
     7681 gcaaaccagg tttccagaat cgcatcagcc tgctgatctt ctttcaccag cgccagcgga
     7741 cgcccgttca tcagcaccag caccagcggt ttgccggtgg ctttcagggc ggcaatcaaa
     7801 tcacgctggc tttgcagaat agtaatatcg gtacggctgg aggcctcgtg cgccatcccc
     7861 tgtgcttcac cgactacagc caccaccaca tcagattgtt tcgccgtctg caccgcttca
     7921 tcaatcatct cttgcggcga acgcggatcg actttgaccg cttcttcata ctgattcagg
     7981 aaatcgataa tgcctttgtc actggtaacg ttcgcccctt tggcatacag cactttaccg
     8041 ttttcaccga cggcattttt aatcccggtc agcacggtca cggattgatc ggcaacacct
     8101 gccgccgacc agctgcccat cacgtcacgt ttactgtccg ccagtggccc aaccaccgca
     8161 atggtggctg attttttcag cggtaacgtt tcgagacggt ttttcagcaa caccaggctt
     8221 tcacgcgcca cttcacgcgc ttctttgcgg tgcaggcggc tttcggcatt ggtatccacc
     8281 gggtcagact ctttcggacc gagatggctg tatgggtcgt taaacaaccc catatcatat
     8341 ttaacgttca gtacatggcg ggcagcatcg tccagctctg ccatcgtcac tttaccggat
     8401 ttgatcaacc caggcagata cttcgagtag tactcgtcgc tcatgctcat gttgattccg
     8461 gatttcagcg ccacgcgcac tgcatcttcc ggatctgccg ccgtgccatg tttaatcagc
     8521 tctttgattg caccgtgatc ggaaacggtg atgcctttaa agccccactg gtcgcgcaga
     8581 acatctttca gcagccagga gtcggaggtg gctggcgtgc cgttcagcga gttcagcgcc
     8641 accatcaccg cgccgctgcc tgcgtccagc cccgctttgt acggcggcat ataatcatta
     8701 aacaggcgct gcggactcat atcgacggtg ttgtactctt taccgccttc taccgcgccg
     8761 tatgcggcaa agtgtttgac gctggtcatc accgagtaac gatctgccgg gcttttaccc
     8821 tgcatcgctt ccaccatggt tttacccatt attgaggtga gatacgtatc ttcgccaaaa
     8881 ccttcggaag cacgtcccca gcgcggatcg cgcgagacat cgaccatcgg tgcccaggtc
     8941 atattcaggc catcatctgc cgcttcataa gcagagacac gtccgaccgt ttttactgca
     9001 tcgaggttaa aagacgaggc cagaccgagg ctaatcggga acaccgtgcg ctgaccgtgc
     9061 agcacgtcgt aagcaaagaa aagaggaatt ttcaggcggc ttaattccat cacctgatcc
     9121 tgcatggcgc ggatatcctg acgggttacg gtgttgaaaa tagccccaac ctgaccgtct
     9181 ttgatcatct cgcggatcgc ctctttcggg ttatccgggc cgacgctgat taagcgcagc
     9241 tgaccaattt tctcatcaac tgtcattttc ttaagcagtt cggtgacgaa cgcatcccgc
     9301 gcttcaggcg ttaatggatg gttaccgaat aaatcatccg ccagtgctgg ctgcagggcc
     9361 agactcaccg cgattcctac tgaacatagc catttcatat ggatttttct tcctttgttg
     9421 cccgacgtgg cagcgaaaat ggtgcaaaaa ccgtagtttg ccataagcat gatggagaga
     9481 gaaaaagaat gctcagttta ttgtctgaat tttcaaaata ttcactcgct gaattgtcat
     9541 acaaggcgct attctagttt gtgatgtttt ttcgccacca caaggagtgg aaaatgtctt
     9601 ccatgacaac aactgataat aaagcctttt tgaatgaact tgcccgtctg gtcggtcatt
     9661 cacacctgct caccgatccc gcaaaaacgg cccgctatcg caagggcttc cgttctggtc
     9721 atggcgacgc gctggctgtc gttttccctg gctcactact ggaattgtgg cgggtgctga
     9781 aagcctgcgt caccgccgac aaaattattc tgatgcaggc tgccaataca ggcctgaccg
     9841 aaggatcgac gccaaacggt aacgactatg atcgcgatat cgtgatcatc agcaccctac
     9901 gtctcgacaa gctgcacgtt ctcggcaagg gcgaacaggt gctggcctat ccgggcacca
     9961 cgctttattc cctggaaaaa gccctcaaac cgctgggacg cgaaccgcac tcggtgattg
    10021 gttcatcgtg cattggcgca tcggttatcg gcggtatctg taataactct ggtggttcgc
    10081 tggtgcagcg tggcccggcg tataccgaaa tgtcgttatt cgcgcgtata aatgaagatg
    10141 gcaaactgac gctggtgaac catctgggga ttgatctggg cgaaacgccg gagcagatcc
    10201 ttagcaagct ggatgacgat cgcatcaaag atgacgatgt gcgtcacgat ggtcgtcacg
    10261 cccacgatta tgactatgtc caccgcgttc gtgatattga agccgacacg cccgcacgtt
    10321 ataacgccga tccggatcgg ttatttgaat cttctggttg cgccgggaag ctggccgtct
    10381 ttgcagtacg tcttgatacc ttcgaagcgg aaaaaaatca gcaggtgttt tatatcggca
    10441 ccaaccagcc ggaagtgctg accgaaatcc gccgtcatat tctggctaac ttcgaaaatc
    10501 tgccggttgc cggggaatat atgcaccggg atatctacga tattgcggaa aaatacggca
    10561 aagacacctt cctgatgatt gataagttag gcaccgacaa gatgccgttc ttctttaatc
    10621 tcaagggacg caccgatgcg atgctggaga aagtgaaatt cttccgtccg cattttactg
    10681 accgtgcgat gcaaaaattc ggtcacctgt tccccagcca tttaccgccg cgcatgaaaa
    10741 actggcgcga taaatacgag catcatctgc tgttaaaaat ggcgggcgat ggcgtgggcg
    10801 aagccaaatc gtggctggtg gattatttca aacaggccga aggcgatttc tttgtctgta
    10861 cgccggagga aggcagcaaa gcgtttttac accgtttcgc cgctgcgggc gcagcaattc
    10921 gttatcaggc ggtgcattcc gatgaagtcg aagacattct ggcgctggat atcgctctgc
    10981 ggcgtaacga caccgagtgg tatgagcatt taccgccgga gatcgacagc cagctggtgc
    11041 acaagctcta ttacggccat tttatgtgct atgtcttcca tcaggattac atcgttaaaa
    11101 aaggcgtgga tgtgcatgcg ttgaaagaac agatgctgga gctgctacag cagcgcggcg
    11161 cgcaataccc tgccgaacat aacgtcggtc atttgtataa agcaccggag acattgcaga
    11221 agttttatcg cgagaacgat ccgaccaaca gtatgaatcc ggggatcggt aaaaccagta
    11281 agcggaaaaa ctggcaggaa gtggagtaaa aattacggat ggcagagtat cgccatccga
    11341 attcacttaa tcgttctgtg ccgtctgccc cgccgccgcc atttgggcgg ctttttgttt
    11401 tttatagctt aacgctgctg ccggaacagg catcacttta ccggtttcaa tccaggtacg
    11461 caggcggctg gcatcggcaa aatgggtgta tttgccaaac gcatccatca ctaccagcgc
    11521 caccggttta ttattgataa ccgtacgcat caccagacaa tggcccgccg cattggtaaa
    11581 gccggttttg gttagctgga tattccagtt atcgcgatac accagatgat tagtattgcg
    11641 gaacggcagc gtatacgtcg ggttagagaa ggttgccata tcttcccggg tagtacttaa
    11701 ctgcccgatc aacggatatt gtttgctggc aatgagcagt ttggttaagt cacgggcggt
    11761 tgaaacgtta tgcaccgaca atccggtagg ttcaacaaag cgcgtgttgt tcattccgag
    11821 cgatttcgct ttcgcattca ttgccttaat aaaggctttg taaccaccgg gataatggtg
    11881 cgcaaggctt gccgccgcgc ggttttctga agacatcagc gccagcaaca gcatatcttt
    11941 acggctgatt tcgctattca gtcgtacgcg cgaatagacc cctttcatct ccggcgtctg
    12001 gctgatatcc acttttagtt tttcatccag cggcagtcgt gcatccagca cgaccatcgc
    12061 agtcattaat ttgctgatag acgcaatcgg acgcaccaga tccgggtggt tcgaatagat
    12121 cactttgttg gtattcagat caacaatcat cgcgctaccg gaggcaattt ccggttgtga
    12181 agcggtggta gcggctgccg ttttcgcaac ggcctgcggt gccaaaggca cagccagcat
    12241 cagggccagg ctaaataaag aaactcgaaa tttcggcatg atgagcattc agatagtggt
    12301 tcacgcgcac gggttgcgca ccgccggagt aaggatttac tgaggctagc gacgccatca
    12361 taacgagcaa aaagtgcgat cgtcaaagga gaatcgtgag gaaatgctgc attgctgaca
    12421 tttacgccag caatgcaacg tcaaagaact ttctagaaca aacgataacc gtagccccat
    12481 agtataacgg taagggcgag cagcacttcc agtaccagca cgccaatcgc cagcgtcgaa
    12541 ctggagaagc taaggccttc ctctttgttg atattcagga agctcggaat accaaggtaa
    12601 agtaggtaac cggtgtaaaa cagtgccacc gtgccgacca gcgcacacaa ccagaccagc
    12661 ggataaagcg ccaccagacc acttaaaaac agcggagttg caacgtagcc cgcgaagacc
    12721 atgcagtgcg caagtgacgg acgctgcgga taattacgcg ccatccacca gatgacccgc
    12781 cccatcaccg cgaccccagc gagcataacg ccataaaaca ggacagccag cgccagtccg
    12841 gtaaaccagg ataacttcag gatagtgcca tcgccaaaat tccagccaat ctgtgtagtg
    12901 ccaatgaagg cgcaaatcac cggaatcgcc gccatcagca aaacgtggtg ggtgtaatgg
    12961 tgagaaatcg tttcgttttc gcgattaatc acctgcattt cacgatcggg atgggaaaac
    13021 agtccccaga catggctcat accgccccct tgttgtgagt tcatgaacct gacagttcaa
    13081 gtataagtca gcttgtgatt attttttgtt cgcccatgta atttgcaccg tctgatgtac
    13141 gtcctttttc agcgtgaatg attcacaggg tgtatgctta caggcaacca aagggagaca
    13201 gactggccta tggatctcaa tacactcatc tcacaatatg gttatgccgc gctggttatc
    13261 ggtagcctgg cggaaggtga aaccgtgact ttgctgggag gccttgcggc acatcagggg
    13321 ctattaaagt tcccgctggt ggtactttct gtagcgcttg gcggcatgat tggcgaccag
    13381 gtgctctatc tgtgcgggcg gcggtttggc ggtaagctgt tacgccgttt ctctaaacat
    13441 caggataaaa ttgagcgggc gcagaaactg atccaacgcc atccgtatct gtttgtcatt
    13501 ggtacgcgct ttatgtatgg ctttcgggtg attggcccga cgctgattgg tgccagccag
    13561 ctgccgccga aaatctttct gccgcttaat attctcggcg catttgcctg ggcgttgatt
    13621 tttaccacta tcggttacgc tggtggtcag gtgattgcgc cgtggttgca caatctcgac
    13681 cagcatttga agcactgggt ctggttgatt ctggtcgtgg ttctggtggt aggcgtgcgc
    13741 tggtggctga aacgacgcgg gaagaaaaag ccggataatc aggcgtaaaa ccattgccct
    13801 ggataaggcg tgcgcgccgc atccgacatc cggttcacca tgcctgatgc ggcgcttgtg
    13861 cgtcttatca tgcctaccaa aattttaccc attgaaatat ccacacacct cactgcgcta
    13921 ttctgggttg aactgcggat tcgccaacat aaagccgcca tccactatca acgactgccc
    13981 ggtggtgtaa tttgcgccct ccgaacaaag ccacgccacc aggctggcaa tctcatgcgt
    14041 tgcgccaaaa cgccgcagag gaatcgaagg ctccgcgtcg ggcttcacgt cgctatcatc
    14101 catgccgttc attggcgtag cgatcgcccc aggtgcgact gcgttcacca aaatcttatg
    14161 cctgaccaac tccagcgcca ttgctttggt taacccgccg agcgcatgtt tagcggctgt
    14221 gtaggcgctg gcatccggca gcggcgtatg ttcatgtacc gacgtaatgt tgatgatgcg
    14281 accaccctgt ccttgtttca ccatctggcg ggcagcaatt tgcgagcata aaaatgcacc
    14341 atcgacatca acggtaaaaa tcttgcgcca ctcatcgaaa gccatatcaa gaaacggcac
    14401 tttggtcatt gcgcccgcat tattcaccag cacatcaata cgccccagcc gttgaataag
    14461 tttctccagc gcctgcgcac cttctggcag attgccgaga tccagttgca cgatctccgc
    14521 acgtacgccg tggctaacta cctcacgcgc ggtatctttt gccccttctt catctgagtg
    14581 ccaggtaatg ccaatatcaa acccttgctg cgccagtaat aacgcgcact ctttgccgat
    14641 ccccgaatcg gaggcggtaa taatcgctac ctgtgccatc gagttctcca cttaacgctg
    14701 aataaacgtt aagtatagaa gtcacatatc atcagcgttt gtaacccccg cccaacgcac
    14761 cagtgagttg aatggaggca tccagccact gcccttgcaa taacaggcca ttggcccgct
    14821 cacgcagcgc ggggattctg gcttcgctga cgcgggaacc agcaatgatg cccgcgttaa
    14881 agcgggcctg cgcaagacct accacacgca aggcatcgcg ctcaatttgc gcctgatgct
    14941 ggtttttctc cgccagtgtc tgaacctgac tggctgcccg cgccacgtca ttcaccgctt
    15001 caaccaccgc tttgttgtag ctggcgatag acaagttgct ttcggctttt gcgatatcga
    15061 gattggcgtt aagacgacca ctatcgaaaa tgggtagcgt caggcctgcc gtaacgccca
    15121 tttgctgcgc ggaatgacgg aacagatcgc ttaagtgcaa cgcatcctgt tgcaggaagg
    15181 ccatcaggtt gatgtcagga taaaatgccg cttttgccgc atcaatggtg cttagcgatg
    15241 actcaacgta ccagtgcgcc gcctgcaaat ctgcccgccg ggccagtaag gagtacccca
    15301 gttcatcagg aagctggctt gccactttcg gcaacgccac cggtttaagc gtcaatgact
    15361 tagtctggtg atttgtgagt gcaattaacc gtgcctcaat aattttcatt ttccccgcga
    15421 catcgttgag ctgctgccgg gttttgctgg cattaatatc ggtttccaca ccttcaactg
    15481 aagaagtaat cccgttctga tatagctggc gatcggtcgc gataatggtg ttctgctctt
    15541 tttcgatttg ctgcaagacc gtgtttaacg ccgcctgggt ttgccactcc cagtacaggc
    15601 gggctacgct gccagccagc aattggcggg tttgctcgcg ttccgccgcc cgtgctttaa
    15661 ccgtacccag gcgggcagta acctccgccc gattctttcc ccagatatcg agatgccagc
    15721 ccgccgttaa gccaaaagtt ccgttggtgt accacggacc ggtcgtacct gcggccggat
    15781 cgttcagagc aaacggcccc attagccctt ctgccgacat tttttgccgc tccatatccg
    15841 cagaaaagtc gatctgcgga ccatcctgag tagcaactgc cttcgcctgg gcttcagcta
    15901 actgaatgcg ctgttcagcc acctgcatat ccggtgcgtt ctgtagtgca ttgttaatta
    15961 aggaagtgag ttgattatcg tgatactcca gccaccattg gctgtctggc caaccatttt
    16021 tcagcgccgt gggtaatgcg gtgtcaactt gtgcagcggg cgtttgctgg cttaacgcct
    16081 ggcgggtttc atgcataggc gcacacccgg ccagcatcag taacagcgga aaacaggcga
    16141 tggctggata aaaggaatca cgattcatgg gggaataatc aggtaagaaa aggtgcgcgg
    16201 agattaccgt gtgttgcgat atatttttta gtttcgcgtg gcaatacatc agtagcaata
    16261 aaacgacata tccagaaaaa tatacactaa gtgaatgata tcttccgatt tatcttaatc
    16321 gtttatggat aacggcaaag ggcttcgttt tttcctatac ttattcagca ctcacaaata
    16381 aaggaacgcc aatgaaaatt atactctggg ctgtattgat tattttcctg attgggctac
    16441 tggtggtgac tggcgtattt aagatgatat tttaaaaatc agatatgaca tataagttgt
    16501 caggtcagga actagatatt ttgtgtactt gatttgcaaa cagctccggt gttggtggcg
    16561 tcaaaatcgc tgaagcgttc cccgaaggcc ggggcagccc acatggatgt gggctgaggg
    16621 cgcgtcttac a
//
LOCUS       DAZKIP010000095        16626 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZKIP010000095 DAZKIP010000000
VERSION     DAZKIP010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.096"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..130)
                     /locus_tag="ACHOUK_003397"
     CDS             complement(<1..130)
                     /locus_tag="ACHOUK_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9898778.1"
                     /translation="MILSTRISLGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALL"
     gene            complement(277..1560)
                     /gene="yeaH"
                     /locus_tag="ACHOUK_003398"
     CDS             complement(277..1560)
                     /gene="yeaH"
                     /locus_tag="ACHOUK_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFP9898779.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            complement(1673..3607)
                     /gene="yeaG"
                     /locus_tag="ACHOUK_003399"
     CDS             complement(1673..3607)
                     /gene="yeaG"
                     /locus_tag="ACHOUK_003399"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFP9898780.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            4043..4789
                     /gene="mipA"
                     /locus_tag="ACHOUK_003400"
     CDS             4043..4789
                     /gene="mipA"
                     /locus_tag="ACHOUK_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFP9898781.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNSNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPIVDKSWTGLISTGITYKF"
     gene            4879..5733
                     /gene="yeaE"
                     /locus_tag="ACHOUK_003401"
     CDS             4879..5733
                     /gene="yeaE"
                     /locus_tag="ACHOUK_003401"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFP9898782.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAVMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELAMLDKAYPAPK
                     GKTALDMV"
     gene            complement(5784..6668)
                     /gene="yeaD"
                     /locus_tag="ACHOUK_003402"
     CDS             complement(5784..6668)
                     /gene="yeaD"
                     /locus_tag="ACHOUK_003402"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFP9898783.1"
                     /translation="MIKKIFALPVIEQISPFLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDANGVALTFELTQSEETKKFWLHDFTLLAHFRVDKNCEIDLEAHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASEMQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(6752..7747)
                     /gene="gapA"
                     /locus_tag="ACHOUK_003403"
     CDS             complement(6752..7747)
                     /gene="gapA"
                     /locus_tag="ACHOUK_003403"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFP9898784.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            8089..8502
                     /gene="msrB"
                     /locus_tag="ACHOUK_003404"
     CDS             8089..8502
                     /gene="msrB"
                     /locus_tag="ACHOUK_003404"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFP9898785.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            8544..8816
                     /gene="yeaC"
                     /locus_tag="ACHOUK_003405"
     CDS             8544..8816
                     /gene="yeaC"
                     /locus_tag="ACHOUK_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFP9898786.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARNNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            9186..10262
                     /gene="ydjL"
                     /locus_tag="ACHOUK_003406"
     CDS             9186..10262
                     /gene="ydjL"
                     /locus_tag="ACHOUK_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFP9898787.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            10289..11668
                     /gene="ydjK"
                     /locus_tag="ACHOUK_003407"
     CDS             10289..11668
                     /gene="ydjK"
                     /locus_tag="ACHOUK_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9898788.1"
                     /translation="MDQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRHTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGTPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            11685..12728
                     /gene="ydjJ"
                     /locus_tag="ACHOUK_003408"
     CDS             11685..12728
                     /gene="ydjJ"
                     /locus_tag="ACHOUK_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HFP9898789.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGTLVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLAMAEQLGATVVI
                     NGAKEDTIARCQQFTEDMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGASAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            12749..13585
                     /gene="ydjI"
                     /locus_tag="ACHOUK_003409"
     CDS             12749..13585
                     /gene="ydjI"
                     /locus_tag="ACHOUK_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFP9898790.1"
                     /translation="MLADIRYWENDATNKRYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIKLFGSDGK
                     AE"
     gene            13590..14522
                     /gene="ydjH"
                     /locus_tag="ACHOUK_003410"
     CDS             13590..14522
                     /gene="ydjH"
                     /locus_tag="ACHOUK_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFP9898791.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHRTALMSRIGKDAAGQFILDHCRKENIDIQSLKQDVNIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFSQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMNICADMIKPRLNETLDDICEALSYVNYAEAKLLTGKETLDEIADSFLACGVKT
                     VVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNLRECAR
                     FANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            14532..15512
                     /gene="ydjG"
                     /locus_tag="ACHOUK_003411"
     CDS             14532..15512
                     /gene="ydjG"
                     /locus_tag="ACHOUK_003411"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HFP9898792.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGFDYIDIYMTHWQSVPPFYTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEVLGR"
     gene            15649..16407
                     /gene="ydjF"
                     /locus_tag="ACHOUK_003412"
     CDS             15649..16407
                     /gene="ydjF"
                     /locus_tag="ACHOUK_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9898793.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            16525..>16626
                     /locus_tag="ACHOUK_003413"
     CDS             16525..>16626
                     /locus_tag="ACHOUK_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9898794.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLT"
BASE COUNT         4332 a         3725 c         4192 g         4377 t
ORIGIN      
        1 aaaggagagc aatgtgagtg ctggatttta ttgatggcag agatatatag ttttgaccag
       61 gatcaatttt cgttaacgat aaaaaatatt taaagaataa ccccagggaa atacgtgttg
      121 acagaataat ttttccgaat gattgttttc tggaactaaa ttgtgtaaag gttatgctgt
      181 catatttaaa gcgattgtaa gctaatgtat gtaataaatg agataactta cagtgagcta
      241 aaaatcagcc aggcgataat gacccggctg atagttttag tctttagctg ttgcattttg
      301 cttatgaaac agttcacgga acaccggata aatatcatcc tggtcgcgga tatgctgcat
      361 cgcaaagttg tcgaaagtag attgcagatg ctcatattct cgccacagtg tctgatgcgc
      421 gcgacgggta atttcgatat agctgtaata acgaacaacg ggtaataatt ttttcgccag
      481 aatttcatgg caaagcggag agtcatcggc ccagttatcg ccgtccgatg cttgtgcagc
      541 gtaaatattc cactgtgccg ggttataacg ctctttcact acctcatcca tcagtttcag
      601 ggcgctggaa acaatggtgc cgccagtttc ctgcgagtag aaaaactcat gttcatcgac
      661 ttctttcgcc tgggtatgat ggcggatgta tacgacttcc acgttcttat acgttctgct
      721 gaggaacaga tacagcagaa tataaaaacg cttagccata tctttagtgg attgatccat
      781 tgaaccggaa acgtccatca ggcaaaacat cactgcctgg ctggagggat cgggccgctt
      841 ctcgtagttc ttgtaacgta aatcgaaggt gtcaataaaa gggacgcgtt caattttggc
      901 acgtaattct gcaatttctt tgcgcagacg ttcctcttcc agcagctgcg caggttcact
      961 gttgctgatg atggccaaat tctcttccag tgcatgaagt tcccgccgct tgcctgccgt
     1021 catggctgtg cgtcgcgcca gtgagttctg caaggaacgc acaacgctga tattggccgg
     1081 aacgccgtta gcggtataac ccgcccgatg cgttttatat tcggtcagct ggcgttgttg
     1141 gttttgtttc agattcggta aggccaaatc ttcaaagagc agatcaagat actcatcttt
     1201 cgaaatctga aagacaaatt catcctgacc ttcaccatcc tggctggcct ggccctgacc
     1261 actgccggaa cctccgccgc caccctgggg acgttcaatt cggtcgttct gaacgaaatg
     1321 gtcattgccc ggatgcacgc ggtggcgcag accgccacgc ccctgatgaa acattggttc
     1381 gctaatatct tccgtgggaa tggatacgga ctcgccgctg tcgacgtcag tcaccgaacg
     1441 cttattaatg gcctcggaga tcgactgttt aatttgcgct ttataacggc gtaaaaaacg
     1501 ctggcgattc accatgcttt tgtttttgcc gttcagacgc cggtcaataa accaggtcat
     1561 atgccccccg tactacattt gccaactgta tgctacgcct gtgttgccat ttctctggcc
     1621 cacggttcgc tgtcgttgta ggcccggtag catgtgtacc gggccagggt tgttaagacg
     1681 atttacgtac gcgcaaatac cattcgcaca gtaaacgcac ctgtttacgg gtgtagcctt
     1741 tttccatcat acggtcgaca aagtcgtcgt gtttcttctg ctcgtcggtt gacgttttgg
     1801 cgttaaacga gataaccggc aacagctcct cggtattgga gaacattttc ttctcgataa
     1861 ccgtgcgcag tttttcatag ctggtccagt tcggattgcg tccgctgtta ttggctctgg
     1921 cgcgcagtac gaagttaaca atctcgttgc ggaaatcttt tggattactg atccccgccg
     1981 gtttctcgat tttctccagc tcggcgttaa gagactcgcg gtcaaacagc tgcccggtat
     2041 ccggatcgcg atactcctga tcctgaatcc agaaatccgc gtaggtaaca taacggtcga
     2101 aaatgttttg cccatattcg gaataggatt caaggtaggc cgtctggatc tctttgccaa
     2161 taaactcggc atatttcggg atcagataac ctttcaggaa ctccagatag cgttctgcct
     2221 gctcttgtgg gaactgctcg cgctcgatct gctgttccag gacgtagaac agatggaccg
     2281 ggtttgccgc cacttctaca tgatcgaagt tgaacacgcg ggagaggatc ttaaacgcaa
     2341 aacgcgtcga cagaccgttc ataccttcat cgacaccggc gtagtcacga tattcctgat
     2401 acgacttggc tttgggatcg gtgtctttca gactttcgcc atcataaacc cgcatctttg
     2461 aataaatgct ggagttttct ggctctttca ggcgcgaaag aatggaaaaa cgtgacagtg
     2521 tttcgagagt gccaggggcg catggggcgt gagtcaattc actgtgatta agcaatttct
     2581 cgtagatttt gatctcttcg gaaatgcgca agcaatacgg caccttcacg atgtaaacac
     2641 gatcgaggaa ggcttcgttg tttttgttat tacggaaagt gacccattcg gactcgttcg
     2701 agtgggcaag aataatcccg ttgaacggca gggcggagat accttccgtc ccgttgtagt
     2761 taccttcctg agtggcggtt aacaagggat gcagcacttt tatcggtgct ttaaacatct
     2821 cgacaaattc catgatccct tggttggcgc ggcacagcgc accggaatag ccgtaggcgt
     2881 ccgggtcatt ctgcgcgtaa tgttcgagtt tacgaatatc gactttccca accagcgcgg
     2941 agatgtcctg gttgttctca tcaccgggtt ccgttttggc gatagcaatt tgttgcagaa
     3001 ttgacggcca gaccttcact acccggaact tagtgatatc gccaccaaat tcatgcaggc
     3061 gttttgccgc ccacggcgac atgatggtgc cgagataacg gcgaggaatg ccatactctt
     3121 tttccagaat ctgcgcatct tcctgcggat tgaaaagaca gaacggatga tcgttgaccg
     3181 ggctacgctc accgttggcg ctcaatacat aaatcggtac gagctgcatt aatgatttca
     3241 gtcgctcagc aagcgatgat ttacccccac ccacaggccc cagcagatac aggatttgtt
     3301 tcttctcttc cagcccctga gccgcgtgtt tcagataaga gacaatctgt tcaatcgcgt
     3361 cttccatgcc gtaaaactct tcaaacgccg gataacgtgc aatgacccgg ttagaaaaga
     3421 gtcgagaaag tctgggttcc tgggctgtat cgaccatgac aggctcaccg atagccatca
     3481 atagccgctc agccgcgttg gcataagcac tgcgatcttg ccgacaagtg gtaagaaact
     3541 cctgcagtgt gaactcttcg tccttggcag cttcatatcg ctggcgatag tgatcgaata
     3601 tattcatggt atgccgtcct ttcgtttttt agcacaggtt aagagccgtt catatgaata
     3661 gtagaggctc ccggaagagg taaactgaac gacgtctctt gctaaaacac agcaactctc
     3721 atgccatttt atcgacgatg agaacaacgt gcccggtgat acaccttcaa aattaagcgt
     3781 agatggcaaa tggaaaactt gcctacacgg ctttcttaat ttcaatggca tatcaacaac
     3841 tcatccataa attttgcata attaatgtaa agaccaggct cgccagtaac gctaaattca
     3901 tttggctgta agcgcggtgt catccgcgtc aggaaaatta aacagttact ttaaaaaatg
     3961 aaaacgtaaa aaggttgggt ttcgatgtat tgacgggtaa actttgtcgc ccgctaaaca
     4021 tttgtttaag gaatgattaa ttgtgaccaa actcaaactt ctggcacttg gagtgcttat
     4081 cgcaacgtct gcaggcgtag cgcacgctga aggtaaattt tccctgggcg caggcgtagg
     4141 tgtcgttgaa cacccatata aagattacga taccgatgtt tacccagtac cggtaatcaa
     4201 ctatgaaggc gataacttct ggttccgtgg cttaggtggt ggttactacc tgtggaatga
     4261 cgcaacggat aaactttcaa ttaccgctta ctggtcgccg ctttacttca aagctaaaga
     4321 cagtggcgat caccaaatgc gtcacctgga tgaccgtaag agcaccatga tggctggtct
     4381 gtcttatgct cactttaccc agtacggtta cctgcgtacc accctggctg gcgataccct
     4441 ggataacagc aacggcatcg tctgggatat ggcctggttg tatcgttaca ccaacggtgg
     4501 cctgaccgtg actccgggta ttggtgtgca gtggaacagc gaaaaccaga acgaatacta
     4561 ttatggcgta tcgcgcaaag agtccgctcg cagcggtctg cgtggctata actcgaacga
     4621 cagctggagc ccttacctgg agctgagcgc cagctacaac ttcctcggcg actggagtgt
     4681 ttacggtacc gcgcgctaca cccgtctgtc tgatgaagtt actgacagcc cgattgtgga
     4741 taaatcctgg actggcctga tttctaccgg gatcacctac aaattctgat aatgcatctt
     4801 tccagggcat tttgtgcata aaaacagggc gtcacgccct gttttgcatt acggtggtgc
     4861 aatacctggg aggaaacaat gcaacaaaaa atgattcaat ttagtggcga tgtctcactg
     4921 ccagccatag ggcagggaac atggtatatg ggcgaagatg ccagtcagcg caaaacagaa
     4981 gttgctgcac tacgcgcggg cattgaactc ggtttaaccc tcattgatac cgccgaaatg
     5041 tatgccgatg gcggtgccga aaaggtggtt ggggaagcat taaccggtct gcgagagaag
     5101 gtctttctcg tctctaaagt ctatccgtgg aatgctggcg ggcaaaaagc gataaatgca
     5161 tgcgaagcca gtttacgccg tctcaatact gattatctcg atctctactt attacactgg
     5221 tctggcagtt tcgcttttga agagactgtt gcagtgatgg aaaaattgat cgcccaggga
     5281 aaaatccgcc gctggggcgt ttcgaacctt gattatgctg atatgcagga actctggcag
     5341 ctgccggggg gaaatcagtg tgccactaat caggtgcttt accatctcgg ttcacgaggg
     5401 attgagtacg atctactccc ctggtgccag caacagcaga tgccggtgat ggcttacagt
     5461 ccgttagccc aggccgggcg gttgcgcaat ggactgttaa aaaacgcggt agtcaacgaa
     5521 attgcacatg ctcacaatat cagcgcggcg caagtattgt tggcgtgggt aatcagccat
     5581 cagggtgtga tggcgattcc aaaagcggcc acgattgccc atgtccaaca gaatgcggct
     5641 gtgcttgagg tcgaactttc gtcagcggaa ttagccatgc tggacaaagc gtatccggca
     5701 ccaaaaggaa aaactgcgct ggatatggtg tgatgttgat acgtgccgga tgcatcgcac
     5761 ccggcacaca acattgacgc aaattaacgt ttggcaacgc gaatggattg cgccagatgt
     5821 gcaggttttt ctttggtcac tttttgcatt tctgaggcgt aagctgtttc tacacaaaca
     5881 aaggtcttgt agccatcgtc cggcatgtcg cccatgctaa ctgaaagcgc cggtcccggg
     5941 ttccagccta caacgttcag atgatgctgg tggcctacgg cgataatacg attcagcgct
     6001 tcatcattaa tcacgctgca atcttgcgga ttcagataca cgcgatcggt acggtcaggg
     6061 aaggtctgaa taccgtcggt cagtacatct tctttcgcgt catttacttt atcaatgaag
     6121 cgatcgccca gcccactgac gcttacctta gcgatatcac ccacgttaaa gtaggtatgc
     6181 agggcagagg tggtttcaaa ttcgccgtgt gcttcaagat cgatttcaca gtttttatcc
     6241 acgcggaaat gcgctaacag agtaaagtcg tgcagccaga attttttcgt ctcttcgctt
     6301 tgtgtcaatt caaaagtcag cgctacgcca ttagcatctt cacgatgtga tttcagcgtc
     6361 cacggcaggt tgcgggcaaa accgtgcgca ggcaggcctt gttgtgccgc cggaccaaac
     6421 cacggccagc aaaccggtac gccaccgcga atagcaacgc catttttgaa cggtgtgttg
     6481 ttgctcaacc acagaacttc ttcttcacct gcaggtttcc acgagagaag gtgtgcgccc
     6541 tgtaatgcaa aagaggcttt tacctgggga tgatcgacta caatgaggtc cagttcatcc
     6601 agtttacgac gggagaggaa aggggagatt tgttcgatga ccggaagggc aaaaattttc
     6661 ttaatcatga cgcagtcctt taacttcatt ttatcaggta aaaaaaagag cgaccgaagt
     6721 cgctcttttt agatcacagt gtcatctcaa cttatttaga gatgtgagcg atcaggtcca
     6781 gaactttgtt ggagtaaccg gtttcgttgt cgtaccagga taccagtttc acgaagttgt
     6841 cgttcagagc gataccagct ttagcatcga acacggaagt gcaaacttcg ccgttgaaat
     6901 cggtagatac tacgtcatct tcggtgtagc ccagaacgcc tttcatttcg ccttcagcag
     6961 cagctttaac ggcagctttg atctgctcgt aagttgcagc tttttccaga cgaacggtca
     7021 ggtcaactac ggatacgttc ggggtcggaa cgcggaacgc cataccagtc agtttgccat
     7081 tcagttctgg cagtacttta cctacagctt tagcagcacc ggtagaggac gggatgatgt
     7141 tctgggaagc gccgcggccg ccgcgccagt ctttgtgaga cgggccatca acggttttct
     7201 gagtagcggt agtagcgtga acggtggtca tcagaccttc gatgatgccg aagttatcgt
     7261 tgataacttt agccagcgga gccaggcagt tggtggtgca ggaagcgttg gaaacgatgt
     7321 cctggccagc atatttgtcg aagttagcgc ctttaacgaa catcggagtg ttgtctttag
     7381 acggaccagt cataaccact ttcttcgcac cagcggtgat gtgtttacga gcagtttcgt
     7441 cagtcaggaa cagaccagtt gcttcagcga caacgtcaac accaacttcg tcccatttca
     7501 ggttagccgg atcacgttca gcggtaacac ggattttttt accgttaacg atcagatgac
     7561 cgtctttcac ttcaacggta ccgtcgaaac ggccgtgagt ggagtcatat ttcagcatgt
     7621 atgccatgta atcagcgtct aacaggtcgt tgattgcaac gatctcgatg tcagaacgtt
     7681 tctgagcagc acggaaaaca atgcgaccga tacggccaaa accgttgata cctactttga
     7741 tagtcatata ttccaccagc tatttgttag tgaataaaag gttgcctgta aaattacaaa
     7801 aaccttacgc agcgtcaagc ggaatcgtgt caatcattgc gacaaatcaa tcctgtgcct
     7861 aagcattacg cgactgactc gcctcactct tcctttgggc ttgagaccac atggggtcgg
     7921 cgccccgaat tttaaagggc aattacgata aaaatgtgat tttcatcacg atttaggtgc
     7981 agccatttcg tcgagatcaa gtttaggaca aaattttgcc agttggtgtt aatgttttgt
     8041 tagaatcagt cacgtaatgt gagcacgatt aaagtgagat gtgagcaaat ggctaataaa
     8101 ccttcggcag aagaactgaa aaaaaatttg tccgagatgc agttttacgt gacgcagaat
     8161 catgggacag agccgccatt tacgggtcgt ttactgcata acaagcgtga cggcgtatat
     8221 cactgtttga tctgcgatgc cccgctgttt cattcccaaa ccaagtatga ttccggctgt
     8281 ggctggccca gtttctacga accggtgagt gaagaatcca ttcgttatat caaagacttg
     8341 tcacatggaa tgcagcgcat agaaattcgt tgcggtaact gtgatgccca tctggggcat
     8401 gtcttccctg acgggccgca gccaacgggc gaacgttatt gtgttaactc tgcttcttta
     8461 cgcttcactg atggtgaaaa cggcgaagaa atcaacggtt gaaaaaacga ttcagcaact
     8521 tattccacag gagcggaaaa attatgaatc ttgatgacat tatcaacagc atgacgcctg
     8581 aggtatacca gcgtttgtcg accgccgttg aactggggaa atggcctgat ggcgttgcgt
     8641 tgaccgagga acaaaaagag aactgcctgc aactggtgat gctgtggcaa gcccgcaata
     8701 ataccgaagc acagcatatg acaattgaca caaatggtca gatggtcatg aagagcaaac
     8761 agcagttaaa agaagatttt ggtatctcag caaagcctat tgccatgttt aagtaagtgg
     8821 ttcatcccct ttttgccgcg gctatgaagg ggatagccaa caatgacccc acgactcatc
     8881 tcaattatct cgccatgacc tggctaagtc ccagtccgat ctcttgccgt cgcgttcacg
     8941 cctgaatgac ttctcctgaa cttaagtctc agacctattt ggccggtaat ccctctcaaa
     9001 tttgctcctt ttgcgttccg ccttacatca acctgtaaca gaaccagcca ttttctgcgt
     9061 aattgtgatc tgagttgtaa aacgcagatt attatctgtg ttttacatgt ttaatgttga
     9121 cctcaaaaat catgtgggta agtatgatga ccatctaagc aaaacaaaac acagaggatg
     9181 acgcaatgaa agcactggct cggtttggca aagcctttgg cggctacaag atgatcgatg
     9241 tgccacaacc catttgtggc ccggaagatg tagtgattga aattaaagcc gcggcaatct
     9301 gcggcgcaga catgaagcac tacaatgtcg atagcggttc tgatgagttt aactctatcc
     9361 gcggccatga gttcgcaggt tgtattgcgc aggttggtga aaaagtcaaa gactggaaag
     9421 tggggcaacg cgtcgtgtcg gataacagcg gtcacgtctg cggcgtttgt ccggcctgtg
     9481 aacaaggtga ttttctgtgt tgtacagaaa aagtaaacct tggtctggat aacaatacct
     9541 ggggcggtgg tttttccaaa tattgtctgg ttcctggtga aattctcaaa attcatcgtc
     9601 atgcgttgtg ggaaatccct gatggtgttg attatgagga cgcagccgta cttgacccta
     9661 tctgtaatgc ctacaaatcc atcgcgcagc aatcgaaatt cctccctggt caggatgtgg
     9721 tcgtcatcgg cactggccca ctcgggctgt tctccgtaca aatggcgcga attatggggg
     9781 cggtaaatat cgtcgtcgtt ggtctgcaag aagatgtggc ggtccgcttc ccggttgcaa
     9841 aagaactggg tgcgacggca gtagtaaatg gttctaccga agatgtggtg gcgcgctgcc
     9901 agcaaatttg tggcaaagac aatctgggac tggtgattga atgctccggt gccaatatcg
     9961 cactgaaaca agccatcgaa atgctccgtc cgaacgggga agtggtacgc gttggaatgg
    10021 gcttcaaacc tcttgatttc tcgattaatg acattaccgc ctggaacaaa agcatcattg
    10081 ggcatatggc ctatgactcc acctcatggc gtaacgctat caggctatta gccagcggcg
    10141 ctatcaaagt caaaccgatg atcacgcatc gtatcggcct gtcgcaatgg cgcgaagggt
    10201 ttgatgcgat ggtcgataaa accgcaatca aagtgatcat gacttacgac tttgatgaat
    10261 aaccactctc ctgaaggaac gttatcgcat ggatcagata acaaaaccgc attgtggtgc
    10321 acggctggat cgcttaccgg attgccgctg gcattcatca atgtttgcca tcgtcgcgtt
    10381 tggcttgctt gtctgctgga gtaatgccgt tggtggcttg atcctcgcgc agctgaaagc
    10441 gttgggctgg acagataatt ccaccacagc cacattctca gcaatcacga ccgccggaat
    10501 gtttctcggt gcgctggtgg gcggcatcat tggcgacaaa accggtcgca gaaatgcgtt
    10561 catcctctat gaggccattc atattgcctc gatggttgtc ggtgctttct caccgaatat
    10621 ggatttcctt attgcctgcc gttttgtgat gggagttggg ctgggagctt tactggttac
    10681 gctgtttgct ggtttcaccg aatatatgcc cggtagaaat cgtggaacgt ggtcaagtcg
    10741 ggtttccttt attggcaact ggtcatatcc gctctgttca ttgatagcga tgggactcac
    10801 gccgctgatt agtgcagagt ggaactggcg ggtacaactg cttatccctg caatattgtc
    10861 gcttatcgct acggcgctgg cctggcgcta ctttcctgaa tccccgcgct ggctggaatc
    10921 gcgcggacgg tatcaggaag ccgagaaagt catgcggagt atagaagaag gcgtcatacg
    10981 ccatacaggt aaacctttgc cgcccgtggt tattgctgat gacggtaaag caccacaagc
    11041 ggtgccatat tcagccttac tgacaggagt attactgaaa cgcgtgatat taggctcttg
    11101 tgtgctgatt gccatgaacg ttgtgcagta cacactaatt aactggttgc caacgatatt
    11161 catgacccag gggattaatt taaaagactc gattgtttta aataccatga gtatgtttgg
    11221 cacgccattt ggcattttta ttgccatgct ggtgatggat aaaattccgc gtaaaacaat
    11281 gggtgtgggg ctattaatcc tgattgcggt gctcggatat atctattcac tgcaaaccag
    11341 catgttgctc ataacgttga ttggtttctt cctgattact ttcgtctata tgtacgtttg
    11401 ctatgcctcg gcagtgtatg tccctgagat ctggccgaca gaggccaaac tccgtggctc
    11461 cggtctggcg aatgcggtag ggcgaatcag tggtattgcc gcaccttatg ccgttgcagt
    11521 gctgctcagt agttatggcg ttacgggagt ctttattctt ctgggggcgg tttcaattat
    11581 tgtcgcaatt gctatcgcca ccattggaat cgaaaccaaa ggtgtctccg ttgaaagttt
    11641 aagtattgat gcagtagcca ataaataata cgggtggatt caaaatgaaa aattcaaaag
    11701 caatattgca ggtgccgggc acaatgaaaa ttatttcagc agaaatacca gtgcctaaag
    11761 aagatgaagt tttgattaaa gtagaatatg tcggtatttg tggttcagat gtacatggtt
    11821 ttgaatcagg tccgtttatt ccgcctaaag acccaaatca agaaattggc ctgggtcatg
    11881 aatgcgccgg gacggttgtg gctgtgggaa gccgtgtgcg caaatttaaa ccgggggatc
    11941 gggtaaatat cgaacctggc gttccttgcg gtcactgtcg ttactgtctg gaaggcaaat
    12001 ataacatctg cccggacgtt gattttatgg cgacacaacc caactaccgc ggcgcattaa
    12061 cgcactatct gtgtcatccg gagagcttta cttacaaact gcccgacaat atggacacga
    12121 tggaagggac gctggtggag cctgccgcag tcgggatgca tgccgcgatg ctggcagatg
    12181 ttaaaccggg taagaagata attattctgg gagcaggttg tattggtttg atgacgttgc
    12241 aagcgtgcaa atgcctggga gcaacggaaa ttgccgtcgt tgatgtgctg gaaaaacgtc
    12301 tggcaatggc ggaacagctt ggtgcgacag tggttattaa cggcgcaaaa gaagacacta
    12361 ttgcacgctg tcagcaattt accgaagaca tgggcgcaga tattgttttc gaaacagcgg
    12421 gttctgcggt caccgttaaa caggcacctt atctggtaat gcgtggcggt aaaattatga
    12481 ttgttggtac tgtacccggc gcttcggcaa tcaatttcct caaaatcaat cgcgaagtca
    12541 ctatccagac ggtattccgc tatgccaatc gttatccggt cacgattgaa gcaatttctt
    12601 cagggcgatt cgatgtgaaa tcgatggtga cgcatattta cgattatcgg gatgtacaac
    12661 aggcatttga agagtcagtt aacaacaaac gcgacattat taaaggcgtt attaagatta
    12721 gcgattaatt aatacgggag aacagaacat gctcgcagat atcagatatt gggaaaacga
    12781 tgccacaaat aaacgttatg ctattgccca ttttaatgta tggaatgcag aaatgttgat
    12841 gggcgttatc gacgcagccg aagaagcgaa atcaccggtt attatttctt ttggcacagg
    12901 ttttgtcggc aacacctcat ttgaagattt ctctcacatg atggtatcta tggcacaaaa
    12961 agcaacggtg ccggtaataa ctcattggga tcatggtcgg agtatggaga ttattcataa
    13021 cgcctggact catggtatga attcattaat gcgtgatgct tccgcatttg atttcgaaga
    13081 aaatattcgt ttaaccaaag aggctgttga tttcttccat ccgctgggta ttccggtaga
    13141 ggcggaatta gggcatgtcg gtaatgaaac cgtttatgag gaagcgctgg cgggctatca
    13201 ctataccgat cctgaccagg cggctgaatt tgttgaacgt acgggctgtg actcattggc
    13261 tgtcgccatc ggcaaccagc atggggttta tacgtcagag ccacaattga actttgaggt
    13321 cgtcaaacgc gtacgcgatg cagtgtctgt tccgctggtt ttgcatggcg catcggggat
    13381 cagtgatgcc gacattaaaa ctgcaatttc gttgggtatc gcgaaaatca acatccatac
    13441 ggagctctgt caggccgcaa tggtcgcagt gaaagaaaac caggatcagc ctttcctgca
    13501 tttagaacgt gaagtacgta aagctgtaaa agaacgggca ttggaaaaaa ttaaactgtt
    13561 cggttctgat ggcaaagcgg aataatgata tggataatct cgacgttatt tgtataggtg
    13621 ccgctattgt tgatattcca ttgcaaccgg tcagtaaaaa tatctttgat gtggattctt
    13681 atcctcttga aagaatcgca atgaccaccg ggggtgatgc aataaatgaa gcaacaatta
    13741 tttctcgtct gggccatcgt acagcattaa tgagccgtat tggtaaagat gccgcagggc
    13801 agtttattct cgaccattgc cgcaaagaga atattgatat tcaaagcctg aagcaggacg
    13861 tcaacataga tacctctatt aatgttggac tggtgacgga ggatggcgag cggacgtttg
    13921 tcacaaaccg taacggcagt ctgtggaaat tgaatattga cgatgttgat tttgcgcggt
    13981 tttcccaggc gaaattatta tcactggcca gtattttcaa cagtccacta ttggatggta
    14041 aagcgctaac agaaattttt acgcaagcta aagcccggca gatgaatatc tgtgccgata
    14101 tgattaaacc gcggttgaat gaaacgctgg atgatatttg cgaagcattg agttatgtca
    14161 attatgccga ggcaaaatta ctcaccggga aagagacact ggatgaaatt gctgacagct
    14221 ttcttgcgtg cggcgtaaaa acggtggtga ttaaaacggg taaagacggc tgctttatca
    14281 agcgtggtga catgacgatg aaggtgccgg cggtcgcagg aataaccgcc atcgacacca
    14341 ttggcgcggg cgataacttt gcttcaggtt ttattgcggc actgttagaa ggaaaaaatc
    14401 tgcgtgaatg cgcacgcttt gccaatgcaa cggcggctat ctcggttcta agcgtcggtg
    14461 ccaccaccgg cgtaaaaaac agaaagctgg tcgaacaatt gctggaagaa tacgaaggat
    14521 aatgaaggca aatgaaaaag atacctttag gcacaacgga tattacgctt tcgcgaatgg
    14581 ggttggggac atgggccatt ggcggcggtc ctgcatggaa tggcgatctc gatcggcaaa
    14641 tatgtattga tacgattctt gaagcccatc gttgcggcat taacctgatt gatactgcac
    14701 caggatataa ctttggcaat agtgaagtta tcgtcggtca ggcgttaaaa aaactgcccc
    14761 gtgaacaggt tgtagtagaa accaaatgcg gcattgtctg ggaacgaaaa ggaagtttat
    14821 tcaacaaagt tggcgatcgg cagttgtata aaaacctttc cccggaatct atccgcgaag
    14881 aggtagaagc cagcttgcaa cgtctgggtt ttgattacat cgatatctac atgacgcact
    14941 ggcagtcggt gccgccattt tatacgccga tcgctgaaac tgtcgcagtg cttaatgagt
    15001 taaaggctga agggaaaatt cgcgctatag gcgctgctaa cgtcgatgct gaccatatcc
    15061 gcgagtatct gcaatatggt gaactggata ttattcaggc gaaatacagt atcctcgacc
    15121 gggcaatgga aaacgaactg ctgccgctat gtcgtgataa tggcattgtg gttcaggttt
    15181 attccccgct agagcaggga ttgttgaccg gcaccatcac tcgtgattac gttccgggcg
    15241 gcgctcgggc aaataaagtc tggttccagc gtgaaaacat gctgaaagtg attgatatgc
    15301 ttgaacagtg gcagccactt tgtgctcgtt atcagtgcac aattcccact ctggcactgg
    15361 cgtggatatt aaaacagagt gatttaatct ccattcttag tggggctact gcaccggaac
    15421 aggtacgcga aaatgtcgcg gcactgaata tcaacttatc ggatgcagac gcaacattga
    15481 tgagggaaat ggcagaggtc ttggggcgtt aaatactcaa cacgattcac ctctgtttct
    15541 gtgaaaactg tgatgaaaat ctgtgattca aacaggttat tttgaaagta aacatcggtt
    15601 atgcgataat cgcgctaatg tgatgtaaaa agatacaggg gtgtaatcgt ggcggcaaaa
    15661 gacaggattc aggcaattaa gcaaatggtt gccaacgata aaaaggtgac agtctcaaat
    15721 ttgagtggga tttttcaggt aaccgaagaa accattcgcc gcgatcttga gaagctggaa
    15781 gatgaaggct ttttgaccag aacctatggt ggtgctgttt taaatacagc gatgttgacg
    15841 gagaatatcc atttttataa gcgcgcttca tcgttttatg aagagaagca gcttattgca
    15901 cgcaaggcac taccctttat cgacaataaa accaccatgg cagccgattc cagtagtacc
    15961 gtaatggaat tgctcaaatt gttacaggac cgtagtgacc tgacgttgct aaccaactcg
    16021 gcggaagcga ttcatgttct ggctcagtca gaaattaaag tcgtttcaac gggtggggaa
    16081 ctaaacaaaa atacactttc actgcaagga agaattacta aagagatcat cagccgctat
    16141 catgtcgata tcatggtaat gagttgtaaa ggtcttgata ttaacagcgg cgcgctggac
    16201 tctaatgaag cggaagctga aatcaaaaag acaatgatcc gtcaggcgac agaagttgcg
    16261 ttattggttg atcactctaa gtttgatcgc aaagcttttg tccagttagc cgactttagt
    16321 catattaatt acataataac tgataaatca ccgggtgcag agtggatagc attttgcaaa
    16381 gacaataata tccagctggt gtggtaattt aagcacgcgt tgtcaatatt atttgttcct
    16441 gcgcctggaa ataaaatgat gataaagatg cggcaagtga atttgccgca attgctacct
    16501 tttttagcaa atccgaggca cccaatggaa caatatgatc aaattggcgc aagactggac
    16561 cgcttgcctt tggcccggtt tcattatcgt atatttggta ttataagctt tagtctgtta
    16621 ttaaca
//
LOCUS       DAZKIP010000096        16563 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZKIP010000096 DAZKIP010000000
VERSION     DAZKIP010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.097"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(116..268)
                     /locus_tag="ACHOUK_003414"
     CDS             complement(116..268)
                     /locus_tag="ACHOUK_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP9898795.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(532..1266)
                     /gene="cysH"
                     /locus_tag="ACHOUK_003415"
     CDS             complement(532..1266)
                     /gene="cysH"
                     /locus_tag="ACHOUK_003415"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFP9898796.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAQLEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKNGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(1340..3052)
                     /gene="cysI"
                     /locus_tag="ACHOUK_003416"
     CDS             complement(1340..3052)
                     /gene="cysI"
                     /locus_tag="ACHOUK_003416"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFP9898797.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(3052..4851)
                     /gene="cysJ"
                     /locus_tag="ACHOUK_003417"
     CDS             complement(3052..4851)
                     /gene="cysJ"
                     /locus_tag="ACHOUK_003417"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFP9898798.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLTAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGVVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQEEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5170..5532
                     /gene="queD"
                     /locus_tag="ACHOUK_003418"
     CDS             5170..5532
                     /gene="queD"
                     /locus_tag="ACHOUK_003418"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFP9898799.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5610..6881
                     /gene="ygcN"
                     /locus_tag="ACHOUK_003419"
     CDS             5610..6881
                     /gene="ygcN"
                     /locus_tag="ACHOUK_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9898800.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDCATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLARFKTHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDTALTGAQAAAQTLISACQHRE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPVLLQRPGWYRTWPALMQDISRDLWDQGDKP
                     VPPLRQLLWRHLRRHGLWNLAGDVIRSVRCL"
     gene            6872..7132
                     /gene="ygcO"
                     /locus_tag="ACHOUK_003420"
     CDS             6872..7132
                     /gene="ygcO"
                     /locus_tag="ACHOUK_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFP9898801.1"
                     /translation="MSVARNLWRAADAPHIVPADSVERQMVQRLINACPVGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7149..7724
                     /gene="ygcP"
                     /locus_tag="ACHOUK_003421"
     CDS             7149..7724
                     /gene="ygcP"
                     /locus_tag="ACHOUK_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFP9898802.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     QIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7872..8732)
                     /gene="ygcQ"
                     /locus_tag="ACHOUK_003422"
                     /pseudo
     CDS             complement(7872..8732)
                     /gene="ygcQ"
                     /locus_tag="ACHOUK_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
     gene            complement(8729..9508)
                     /gene="ygcR"
                     /locus_tag="ACHOUK_003423"
     CDS             complement(8729..9508)
                     /gene="ygcR"
                     /locus_tag="ACHOUK_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFP9898803.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAHGNSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFASE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDAPFI
                     TLEQRTEHGLRCCRIRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAELPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9486..10822)
                     /gene="ygcS"
                     /locus_tag="ACHOUK_003424"
                     /pseudo
     CDS             complement(9486..10822)
                     /gene="ygcS"
                     /locus_tag="ACHOUK_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417251.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(10916..12370)
                     /gene="ygcU"
                     /locus_tag="ACHOUK_003425"
     CDS             complement(10916..12370)
                     /gene="ygcU"
                     /locus_tag="ACHOUK_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFP9898804.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVNCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12440..13225)
                     /gene="ygcW"
                     /locus_tag="ACHOUK_003426"
     CDS             complement(12440..13225)
                     /gene="ygcW"
                     /locus_tag="ACHOUK_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9898805.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     VFIPSFVKDNGETKEMIENQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASQASNYVNGHLLVVDGGYLVR"
     gene            13544..14821
                     /gene="yqcE"
                     /locus_tag="ACHOUK_003427"
     CDS             13544..14821
                     /gene="yqcE"
                     /locus_tag="ACHOUK_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFP9898806.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSTSLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTQPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            14848..16326
                     /gene="ygcE"
                     /locus_tag="ACHOUK_003428"
     CDS             14848..16326
                     /gene="ygcE"
                     /locus_tag="ACHOUK_003428"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFP9898807.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMEKFNIPRHMLFDVQMPGTVLGQITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKALTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
BASE COUNT         3931 a         4370 c         4174 g         4088 t
ORIGIN      
        1 attcataatg ataaatagag actatatatt cacataaaaa atataaattt aaaaaagggt
       61 gcaggagacc aaaccatcac aattcaatac gcggggtttt acccccgcgt ggtggctact
      121 tcttcgattc gtaagcgaga actgccttaa actccatacc acgctctctg atactcaact
      181 cacataacga gtcgcctacc atgagcgtga atcccagtac tgtacaacac agtactatga
      241 ttgccacaag ggcatatttt gttagcatgt cttgcctccc tgcaaagaag agactaacat
      301 ccgaattgct acgttcgaac gcgagcctca ggttgataga aatatcgcct ggggcttttg
      361 tccatctgga acctcgcgaa tgcttaacgc cagacagcct caagcacccg acgccattct
      421 atacctgata attcttccag cggcgttcac gtttaaaatc aggaatgctg ctcgcaaaat
      481 aaaaacgccc ccatcattac tgacagaggc gtttaattta tccggcaata tttacccttc
      541 gtgtaaccca cattcccttt ttaagccaaa gaaacgcgtt tcttcttccg ccatgccggg
      601 ttcccattta cgggttgtat gggtatcgcc caccgataaa tatccttcgt cccataatgg
      661 gtgatatttc aggccattct tttgcagata ctgatatatc gttcggttat cccagtcgat
      721 aatcggcagc actttaaata cgccacgctg aattgccagc accggtaaat tggcgcgact
      781 gccggattgt tcacggcgca ggccagcaaa ccaggtttgc gcgttaagtt ctttcagcgc
      841 ccggttcatc ggttcgactt tgttgatgtc attgtacttt tcaatgcctt caacgccctg
      901 ttcccacagt tttccgtagc gtgcttcctg ccaggctgcg ctttcggtag cacggtacac
      961 tttcaggttg agcttgagtt tgtccgttaa ctcgtcaata aagcggtagg tttccgggaa
     1021 caagtaaccc gtatcggtga ggatcaccgg aatatccggg cgaatttgat tcaccagatg
     1081 caggctcacc gccgcctgaa tgccaaagct ggaagaaagt acatattcac cgggcagatt
     1141 atccagcgcc caggctacgc ggccttcagc gtccagtttt tccagttggg cgttagtttc
     1201 cgccagcgcc agaatgcgat ctaccttcgg cagttcgttc agggcgttta gatcgagttt
     1261 ggacatagat tcctcactgt ttgccttgcc tgatgcgacg ctcacgcgtc ttatcaggcc
     1321 tacaagaccg ggctgatggt taatcccaca aatcgcgcgc cggatcgagc accgggcgaa
     1381 tgatgcccgc acgcaccgta aagtcgccga agccttcacc cgcttcgcgc tctttcgccc
     1441 agcgccctat cagttcatca agcgacgcca ggatttccgg ctcggtgatg ttttctttat
     1501 acatccgtgg gatacgtgtc ccaatgcggt tgccgccaag atgcaggttg tagcgacccg
     1561 gcgctttacc caccaggccc acttccgcca gcatcgcgcg accacaaccg ttcgggcagc
     1621 ctgttacacg catcacgata tgctcatcgc tgacaccatg tttcgccatt aaattatcga
     1681 tgttgtcgat aaaagacggc aggaaacgct ccgcttccgc catcgccagc gggcaagtcg
     1741 ggaacgagac gcaggccatt gagttttcac gctgcggcgt gacggcattc attaacccgc
     1801 tctctttggc gatcttctcg atcttcgctt tctcgctttc cggcacaccg gcgatgatca
     1861 gattctggtt cgccgtaatg cggaaatcac ctttgtggat cttcgcgatc tccagcaggc
     1921 cggttttcag cggacgcccc ggataatcaa ggatgcgacc attttcgata aacagcgtca
     1981 ggtgccagtt atcatcaatg cccttaaccc agccaatacg atctccgcgt ccggtgaact
     2041 catacggacg gatcggttca aatttgatcc ccgcgcgacg ctccacttcc gctttaaacg
     2101 tctcaacccc cacgcgctcc agcgtgtatt tggttttggc atttttacga tcggttcggt
     2161 taccccagtc acgctgagtt gtcacgacgg cttccgccac tgccagcgta tgctccagcg
     2221 gcagatagcc aaactcactc gccgtgcggg cgtaggtttt cttgttgccg tgttcgatag
     2281 aaagtccacc gcccaccaac aggttaaagc ccaccagctt gccgttttcg gcgattgcca
     2341 cgaagttcat gtcgttggcg tgcagatcga tatcgttctg tggcgggatc actaccgtgg
     2401 ttttgaattt acgcggcagg taggtctggc cgaggatcgg ttcttcatcg gtggtggcga
     2461 ctttttcctg atcgagccag atctccgcat acgcgcgggt acgaggcagc agatgttcag
     2521 agattttctt cgcccactcg tacgcttctg cgtgcagctg cgactcgtaa gggttcgagg
     2581 tgcagagtac gttacggttc atgtcgttag cggtcgccag cgcatcaaga ccgaccgagt
     2641 gcagcatctg gtgcaccggt ttgacgttct ttttcagaat gccgtggaac tggaatgtct
     2701 ggcggttggt caggcgaatg ctgccataga tggtgttttc accggcaaat ttatcgatcg
     2761 cctgccactg tttggtggtg ataactccac ccggcagacg gcagcgcaac agcatcgcat
     2821 ggcgcggctc cagcttctgt tcagcacgtt cggcgcggat gtcgcggtca tcctgctgat
     2881 acatgccgtg gaagcggatc agcaggaagt tatcgccctt aaagccgccg gtcaggccgt
     2941 cgtttaaatc ttccgcaatg gtgccgcgca ggtagttgct ttcaagcttc atgcgctcgg
     3001 cgtctgtcag ttttccttcg accactaaag gccctggatg tttttcgctc attagtagac
     3061 atctcgctga taacggcgct ctacgcgcag ctcacttaaa aattcatccg ccgcttcggt
     3121 gtccatgcca ccaaattcgg caatcacttc cagaagtgcc tgctcaacgt ctttcgccat
     3181 gcgattagcg tcgccgcaga cataaatgtg ggcaccgtca ttgatccagc gccacagctc
     3241 cgcgccctgt tcgcgcagtt tgtcttgtac gtaaactttt tctttttgat cgcgcgacca
     3301 ggcaagatcg atacgcgtca gcacgccctc tttgacgtaa cgctgccact ccacctggta
     3361 gaggaaatct tccgtaaagt gcgggttgcc aaagaacagc cagtttttac ccggcgcttc
     3421 gtcagcggcg cgctgctgca taaaggcgcg gaacggcgcg atgccggtgc ctgggccaat
     3481 cataatcacc ggggtttccg ggttagcggg caggcggaag ttatcgttat gttcgataaa
     3541 tacgcgaact tcgccctctt cttccacgcg gtccgcgagg aagctggagg caccaccggc
     3601 acgggcgcgg ccttccacgt cgtaacgcac cacaccaacg gtgacgtgta cttcgttctc
     3661 gacttcctcc tgcgaggagg cgatggaata cagacgcggc gtcagcgggc gcagcagatt
     3721 aatcagcgct tcggcgtcca gttgcgccgg agagaagcgc accatgtcga caatcggcgt
     3781 agtcgcggcg taatgctgta acttcgcttt atcgcccacc agcggcaaca gcgtttcgct
     3841 gcgggtaagc gtggcatagt tctcaacaat attggcggtg ttgacggtca gttcgaagtg
     3901 ccactgtagc gcttcgttca gaggcaacgt tttgccctcg acggtgacag gttcatcgcc
     3961 tttcagccac agcagttcga caagttcttt caccagtgcc ggatcgttct gataccagac
     4021 acccagcgcg tcacccggct ggtaacgcag gcccgagtca cctaagtcaa tttcgatatg
     4081 gcgaacgtct ttttcagagt tacgcccggt aattttctgg ttaaccgaaa ggctcgctac
     4141 cagcggcgcg tctttgctgt acgggctggt gtggatttca tttaccacgc cagtagcgac
     4201 ggattgcgaa ggtgccgcga caggcgcacg cgatttaagc gcatcaacca cgcgggcgcg
     4261 ccactcgctg gcagcagcct ggtattcaac gtcggcgtcg acacggtcga gcaggcgttc
     4321 accgcccagt tccgccagct tgctgtcgaa atctttcccg gactggcaga aaaattcata
     4381 agaactatcg ccgaggctaa acacggcaaa agcggtattt tccagcttcg gcgctttttt
     4441 ggagaacagg aacttatgca gcgcgacggc ttcttccggc ggttcccctt ctccttgcgt
     4501 tgacgtcacc acgataagca gtttttcgct ggcgatttgt ttgaatttat agtcgcccgc
     4561 gttcaccagc ttaacgttca gttttgccgt caataaatca tcgcgtaatg cttcagcaac
     4621 ccggcgcgca ttgccggttt gcgaagcaga gataatagtt atacccggca tttctgcggc
     4681 tggcgctggc gtcgctgcaa gcgcagcagg ctgctgattg agcacgcccc agaaatagcc
     4741 agaaacccag gcaagctggg tgggagttaa atcggtcgtg gccgcctgaa ggcgtgccag
     4801 ttgctccggg ttcaacggaa gcaacgcgga aggtgggacc tgtgtcgtca tgcgtcgtta
     4861 tgttccagta agcaaagctg tttctgcgcc ctgtcagcgc ccataaaaca gaagagaagg
     4921 taaggttaac ggggtgaacg gtgtggatta aagacgggat agcgataact aataaccaaa
     4981 tcgactaacc tgttttagca atagtcttta acaacaaaat agattaacca acctaatgaa
     5041 aaacaaatga atttagccaa tcattaagat aaatcagcga ttttgcgcaa caaagtcgtt
     5101 ttagataatg cgaaaaaaga gcctttccgg tactctacgg cggttttatc gtcctgtaga
     5161 gaaattatga tgtccaccac gttatttaaa gatttcacct tcgaagccgc tcaccgctta
     5221 ccacacgtcc cggaagggca taaatgtggt cgcctgcacg ggcattcctt tatggtgcga
     5281 ctggaaatta ccggggaagt cgatccgcat acgggctgga ttatcgattt cgctgaacta
     5341 aaagcggcgt ttaagccaac ttatgagcgc ctcgatcacc attatctcaa tgatattcca
     5401 ggtctggaaa acccaactag cgaagtttta gcaaaatgga tttgggatca ggttaaaccc
     5461 gttgtgccgc tgttaagtgc ggtgatggta aaagaaacct gcaccgctgg ttgtatctat
     5521 cgcggcgaat gataagagtg tggcggcggt caatttccct taagtaacgc tatgttaggg
     5581 tgttgtgttc aggatatctg gggcatgaca tggaagacga ctgcgacatt attattattg
     5641 gtgccggtat tgcaggcacc gcttgcgcgt tacgctgcgc gcgagcgggt ttatccgttt
     5701 tgttactgga acgcgctgaa atccccggca gcaaaaatct ttccggcggg cggttatata
     5761 cccatgcact cgcggaactc ctccctcaat ttcatctgac cgcgcctctt gaacgacgca
     5821 tcactcacga aagcctttcc ctgttaacgc cggattgcgc aacgacgttt tccagcttac
     5881 agcccggcgg tgaatcctgg agtgtattac gtgcacggtt cgatccgtgg ctggttgccg
     5941 aagccgaaaa agaaggtgtc gaatgcatcc ctggtgcgac ggtggatgca ctgtatgaag
     6001 aaaacggcag ggtgtgtggt gtcatttgtg gtgacgatat tctccgcgcc cgttatgtgg
     6061 tgctggcaga aggtgccaac agcgtcctgg ctgaacgtca cgggttagtg actcgtcctg
     6121 ctggcgaagc gatggcgttg gggatcaaag aagtgctgtc gctggaaaca tccgctattg
     6181 aagaacgttt tcatctggag aataacgaag gcgcagcgtt gctgttcagc ggcggaatct
     6241 gtgatgactt acccggcggc gcatttcttt atactaatca acaaacgctc tcgttaggga
     6301 ttgtttgccc actctcttcc cttacgcaaa gtcgtgttcc ggcaagcgag ctgcttgctc
     6361 gctttaaaac gcatccggca gtgcgcccgc ttatcaaaaa cacggaatca ctggagtatg
     6421 gtgcgcatct ggtgccagaa ggtggcttgc acagtatgcc ggtacaatac gccggtaacg
     6481 gctggctgct ggtgggcgat gcgttgcgca gttgcgtcaa taccggaatt tctgtgcgcg
     6541 gcatggatac ggcgctaact ggcgcgcagg cggcggcaca aacactgata agcgcctgcc
     6601 agcaccgcga gccgcaaaat ctgtttccgc tttatcatca caacgtagag cgcagcctgc
     6661 tgtgggatgt tctacagcgt tatcagcatg ttccggtgct tttgcaacgc ccgggatggt
     6721 accgtacgtg gcctgcgtta atgcaggata tttcccgcga tttatgggat cagggtgata
     6781 aacctgttcc accgctgcgc cagttactct ggcgtcattt acgtcgtcat ggcttgtgga
     6841 atctggcggg cgatgttatc aggagtgttc gatgtctgta gcccgtaatc tctggcgcgc
     6901 tgctgatgcg ccgcacattg ttccggctga ctccgttgag cgtcagatgg tacaacggtt
     6961 gattaacgcc tgtccggtag gtcttttttc gctcacaccg gaaggtgact tacgcgttga
     7021 ttatcacggc tgcctggagt gtggcacctg ccgtttgctg tgcgacgaat ccacactaca
     7081 acagtggcgc tatccgcctt ccggattcgg catcacctac cgatttggat aaataataag
     7141 gataatttat gcccctctta cacctgctcc gccagaatcc ggtgattgct gccgttaaag
     7201 ataatgccag cctgcaactg gccatcgatt ctgaatgcca gtttatttcc gtcttgtacg
     7261 gcaatatctg caccattagt caaatcgtta aaaagattaa gaacgctggg aaatatgctt
     7321 ttattcatgt tgatttgctg gaaggcgcgt caaataaaga agtggtgatt cagtttttga
     7381 aactggtgac cgaggcggat ggaattatca gcaccaaagc atcgatgcta aaagcggcaa
     7441 gagcggaagg ttttttctgt attcatcgcc tgtttattgt tgattcgatt tcgtttcaca
     7501 acattgataa gcaagttgcg caatcgaatc cggattgtat tgagatcctg ccaggctgca
     7561 tgcccaaagt gctgggctgg gtgacggaaa aaatccgcca accgctgatt gctggcgggc
     7621 tggtatgcga tgaagaagat gcgcgtaatg cgattaacgc gggtgtcgtg gcgctttcca
     7681 ccacgaatac cggagtctgg acgttagcga aaaaattgct ttgacgggat aatcgtaacc
     7741 aattgaattt ggtttgattt gtaggccgca cgccacatcc gacattcagc gcctgatgcg
     7801 acgcttaaca cgtcttatca ggcctacagg tccccagtcc agaaccgtag gcaggatcat
     7861 ccggcagcac gttattggca ttctgcgtca atgtttgtca ccagtgcctc cagcatcact
     7921 ttccagtcat caaccacgcc aacatctgcc tgtgaaaata ccgctgcgct ggcgtcatga
     7981 ttaatcgcca ccacaaactt gctattgcgt acgcccgcca ttaacgctgc tgcgccagaa
     8041 gcgccaacaa caatacacac ctcaggtgcc agcagatgtc cggaaatacc aatcactttt
     8101 tcagcatcaa cgccaccgtt catcactcgc gcccgactgt agcccacttc ggctcccagc
     8161 ttttatgcca gcatagcgat ttcctggtta tctgcctctc cgccctgccc caccaccaac
     8221 acccgtctgg cctcagctaa tgggtcgcgg gtaacatttt tcaggttctc tatgctaacc
     8281 agccagtccg gaagcgcacc tgggactatg ttcagttgct gcataccaga gggcaacgtg
     8341 gcatttttat ctgctcccgc ctggcgcgcc agggaaagac atagcgggcg tttttctgtt
     8401 tgcagcgtcg ccgttagcgc atttccccag tgggatttgc gcacgcttac gattggaatc
     8461 tctaacgaag tcacctggca gatgcttgca ccatgtaaac gccaggccag ccgagtcgac
     8521 agttcatcgc caaacgttcc cggcggaaag agaacaacgt tcgcaggcgt tcgctgccac
     8581 tgttccacca gcgcatccag cacctgttcc accactacgg gctgtggttc tatttgccag
     8641 tgcgccaggg tacaaccgct gaaatcctgc gccgccagcc agcccgctaa tgcggcattc
     8701 tcctgattta ttgtcacaat tgcgatattc atggctgcat cctttggcgt aaatagtcct
     8761 gccatagctt ttgcgctttt tctgctaccg tttgcccatc aatcagcgtc gctccgcgtc
     8821 gttgctctgc tcttgccagt tgcagacact gcatcgctgg caattcggca gcaacagttt
     8881 tacggataat ttccgctttc cccgcagcca ttcgctggcg cattcccggt actggcaagg
     8941 ccacttcacc gcactgacgc acggcaatca ccgcaggcaa gcgaatccga caacagcgca
     9001 gcccatgttc agtacgctgt tcgagggtga taaacggcgc gtcgagagtg aaacgctcca
     9061 cctgggtgaa gcaaggccag cccagcattt ccgccagcaa aaatggcgtt tgcccattct
     9121 gcccttcgct gctttggcag ccggtaatta tcagatccag cggattctga cgctgccatt
     9181 cggcaatatg gcgagcgaca aattccgacg caaagcgcag atctgccgcc gtctccagca
     9241 atacagcttc ctcaaaccca agagccatga gatagcgcag ccagtgcaac gcccgttcat
     9301 cccccatgct caacgcggtt aaagacatcg gcgtaccgtt ttttctctgc gccaacagca
     9361 gcgcggcggc agcctgttca tcagcaccga gtaaacttcg cagtagcgaa atatccggtc
     9421 cgctattacc atgagccgcc gcctgccact ctttttccgc cagcattccg gcatccggtt
     9481 cggctttaaa cgctaacaga atgttcattc gcacctccta catttcccgc cgccaccagc
     9541 gggagtgctt tggtttctgg tgcccatagc caggttacaa caaaaccagc caacaacact
     9601 gccgccagga gcaataaggt gacttgcatt ccccactgcg ccagcaccca cggcagcagg
     9661 ccagtactta ccgccgcccc caaccgactc atggcggtgg caaaaccgac gcccagcgag
     9721 cgaatgtcag taggaaaact ttccgcaggc aaaatgccca ccagattact gactgccgaa
     9781 atggtggtgc tgaagagaac aaaagcagca gcgttaatga actgccggaa ggcaaacagg
     9841 ccatcactac cagcgttgcc gccagcagca aaaaacttcc cagcaaaaat ttgcgatgtg
     9901 ccagcaggtg cgtcagaact aatcccagca gcgcgcccac aattaacaac gcattaagca
     9961 tcaggctggc agtcagcgca tcttccagtc caatagtctg ggcgatagtt ggcagccagg
    10021 tataaatcac aaaccatggg attacgaggc agacaaagaa gacgctgtta aacgccgtgc
    10081 gccgccagta gcgcgaagag aacaaggttt tgatgtgttt atgggtcgcc gttgccactt
    10141 catcgcccag taaaacatgg ggaccaaaat agcgatgcac gatagcgtga gcttctgcaa
    10201 aacgcccctg gcgcagtagc cagcgtggcg attctggcgt tccccagcgt aataacgtaa
    10261 tcaacaacgc gggcagagct gccgatgcca gcagccagcg ccaggcctcc gggttttcgg
    10321 aaataaagtg atgtccggca atacttgcca acacatagcc tacggtccac accacgctga
    10381 atgcgcccag caaaacaccg cgatggcggc gcggggaaaa ttcagccagc aaggtgtgac
    10441 ctactgagta atcgcctccc agaccaatgc cgatcaaaat gcgcagtcca ataagatgct
    10501 ctggcgtggt ggcaaaaaat tgcaagaacg aagcgagcgt aatcagcaaa aagctgaagg
    10561 tgaagatttt ttgccgacca atatggtcgg agatccaccc aagaaccagg ctgccaagaa
    10621 acaaaccaag gtgcgccgag ccgccgatca ttcccgccat aaacggcgtc agttgcatgg
    10681 cgggcgtaag ttgaataatg gcgtaaccaa tgacgccgag aacataaccg tcggtcaggt
    10741 gtgcgccgaa agtgagcgca gcaattcgac agtgaaaacg gttaagcggt aaatcatcca
    10801 ttcgcaccgg tgaagtgttc atctttaccc cttcattgtc cggtaataag aacggcttct
    10861 cgcctgagaa gccgaaaagg caacgcccca tcatcgcgga acgctgcctg acacattatt
    10921 tttcaatcgg atagatagta cccgtattca taatgccatt aggatcgaac tgctttttca
    10981 gcccttccag caacgcccac gcgctgccgt gttccagctt gctccagtga acgcgatgtt
    11041 taccgatacc gtggtggtgc accatcgaac caccgaggcg aatggtttct tcgcaaatga
    11101 ttttgttgag cggattgtgg tacttgtcga tttcctcttc cggcttacag ttgacaacgt
    11161 tgtagtcgta gacgaagtac atgttggtgc cgttctgata gctatgagag gagtgaccgc
    11221 ccagcatggt gatgtcgtcg gcgtgcggga actcggtacg aatacggtta ataacgcttt
    11281 cgtagatttc gtggatgcag ctccagcagc cggacacttc ggtagtaaag cccatgttgc
    11341 cggttttgag gatctgcaca cgttcggcag ccactttatc cggtccccag ttcaggttgt
    11401 taaaccaggt ttcgatcagc ttgctgtcca cgcgttggca ttgcgggtag cgggcaacga
    11461 tttccgcaat cccttcgccc gtcgccttcg caatgcgagg gttaccttca gccataaaga
    11521 tcagcacgca ttttccgtcg gcaaaatggg tgaagtgttg ggtgccatct tcagcgtcat
    11581 acaaacgagc gatcgacgga cgataccctt ccaccatgat ttcacgcagg atgttgaagc
    11641 cggttttcat gtcttccagg atatagccgt agaagaggtt gttttccggg gtgaatttaa
    11701 agattttcac tgttacttca gtgatatagc acaatgcacc ttcgttgccg atgatgatgt
    11761 gacgaatgtc cgggccagcc gcgcggcgtg gcacgttttt aatgcgtgtg acggtgccat
    11821 ctgccaacac tgcctccaga ccaacgacca tatcttcgat tgcgccgtag agtgtggaga
    11881 actgcccgat actgcgggtt gctaccaggc cgcccatctg cgccagcggc tttgactgcg
    11941 gagaatgccc cgtggtgtaa cctttttcac gcaacgcgtt ttccagcacc tccagcggaa
    12001 caccacattg cgccgtcgcc tgcatattct caatatcaat attaatgatt tgattcatgg
    12061 cggaaccgtc gagcaccacc gagttttcta caacagtttc cagcccacct tcggtggcgg
    12121 aagcaccggt acgcggcaca ccgttaattt tgtgcgcatt cataaaattc agcacacggg
    12181 atacttgctc tgtggaaccg agttttacga ccgctgccgg aatcggcaaa gtataaatgc
    12241 catgaatatc cggaaattta cgaaaacggt caatactgtt cttctttaat actgtttcat
    12301 cggtaattac gcgatctgca ccaacaattt cctttagctg gtcgacaatc gctgcgcgag
    12361 ataaagacat agtaattcct tcctgataat aaaaatggaa taatcattaa taagagaaaa
    12421 cagaaaccac cgttatgggt tagcgcacta aataaccgcc atcgaccact aataaatgcc
    12481 cgttgacata attcgatgcc tgacttgcga gaaacacggc tgcgcccatt aaatcctgag
    12541 tatcgcccca acggtttgcc ggaatatgat caagaacgcg ctgattggtt tctggattac
    12601 tgcgtgtcgc cagcgtaatg tctgttgcat aatagccagg ggcgatacca tttacctgaa
    12661 tattatattg acctagttca tcacaataag ctttggtgaa tccggcaaga gcatgtttag
    12721 tagcagaata tgcaggtgac cattgtccac ctaagtaaga gaacaatgaa cagatattaa
    12781 tgattttacc gcttttctgc gggatcataa tttttgccgc ttcgtagctt aactcgaatg
    12841 cggcggtcag gttcacatca atcatcgggt cccagtcggc acgaccgaag tccagcacct
    12901 tattcagctt acaaataccg gcattgttaa ccagaatatc aactgtaccg aaacgctcac
    12961 agcaggcagc gataatcttc tgcggcgcgc cttctgcggt gatatccacc tgcatgaagt
    13021 ccacctcaac accctgattt tcaatcattt cctttgtttc gccgttatct ttgacaaaac
    13081 ttggaataaa gacatttgcg cctgctttgg ccaacgccat ggcaaatgcc tggcctaaac
    13141 cgctattccc gccggtaaca attgcggttt tacctttcag ggagaaaaaa tccattgaga
    13201 acgcattgag agattcgatt gacatagtta gctccaattt ccgtaaggca aaaaaaaaga
    13261 aaggtaactt cccccgcgag agggaagtta cctttctcat tatctctggt aactggagat
    13321 aatttttagc ataaggacat aactgctaat gtgatcgtaa tcacagtgtg atattcgtat
    13381 tatcaaatag ttttaaaaag cagaagcgtg attactctca cacaacacct ctgctttcca
    13441 tgttatttat tatttcaatt actagagacc tgagaaaagt aatccacctg aaagggcggc
    13501 ttacttttct cttttttttg tctattacaa atgggaatac gatatgcaac acaactcata
    13561 tcgccgttgg ataaccctcg cgataattag ttttagcggc ggcgttagtt tcgacctggc
    13621 ttatttacgt tatatttatc aaattcccat ggcgaaattt atgggattca gcaataccga
    13681 gataggttta ataatgagta cctttggtat tgcggctatt attctttatg cccccagcgg
    13741 cgttattgcc gataaatttt cacaccgcaa aatgattact tccgcgatga tcattaccgg
    13801 attactgggt ctgttaatgg caacgtatcc accgctgtgg gtaatgctct gtattcaggt
    13861 cgcctttgcg ataacaacga ttttaatgct gtggtcggtg tcgattaaag ccgcatcgtt
    13921 gcttggcgat catagcgagc aagggaaaat tatgggctgg atggaagggc tgcgcggcgt
    13981 cggtgtaatg tcgctggcag tgtttaccat gtgggttttt tctcgctttg caccggatga
    14041 cagcaccagc ctgaaaacgg tcattatcat ctatagtgtg gtttacatct tgttggggat
    14101 tctgtgctgg ttttttgtta gcgataacaa caacctgcgc agtgccaata acgaagaaaa
    14161 acagtcattc cagcttagcg acatccttgc cgttttgcgt atcagcacca cctggtattg
    14221 cagcatggtg atttttggcg tcttcaccat ctacgccatt ctgagttact ccaccaacta
    14281 tctgaccgaa atgtatggca tgtcgctggt ggcggcgagc tacatgggga ttgtgatcaa
    14341 caaaatcttc cgcgcgctgt gcggcccact tggcggcatt atcaccacct acagtaaagt
    14401 gaaatcccct acccgcgtga tccaaatcct ttccgtactc ggcctgctgg cgttaactgc
    14461 cctgctcgtc acgaactcta acccgcaatc ggtcgcgatg gggattggcc tgattttact
    14521 gctgggattc acctgttacg cctcacgcgg gctgtactgg gcctgccctg gcgaagcgag
    14581 aacaccgtct tacattatgg gcaccacggt aggtatttgc tcggtgattg gattcctgcc
    14641 ggatgtcttc gtttacccaa ttatcggcca ctggcaagac acccagcccg cagcagaagc
    14701 ctaccgcaat atgtggctga tgggcatggc ggcgcttgcc atggtgattg tctttacctt
    14761 tttgctgttc caaaaaattc gtactgctga tagcgccccc gcaatggcca gcagcaagta
    14821 agccaggaaa attacaggag aaaaatcatg tcgaagaaat acatcatagg gattgatggc
    14881 ggaagtcaga gcacaaaagt ggtgatgtac gatctggaag gtaacgtggt ttgcgaaggc
    14941 aaaggtttat tgcagccgat gcacacgcca gatgccgata ccgcagaaca tcctgacgac
    15001 gatttatggg catcattatg ttttgccggt cacgatttga tgagtcagtt tgccgggaat
    15061 aaagaagata ttgtcggtat tggtctggga tccatccgtt gctgccgtgc gttattgaaa
    15121 gccgatggca cgcctgctgc gccgttgatt agctggcagg atgcacgcgt aacgcgtccc
    15181 tacgaacaca ctaatcctga cgtggcctat gtcacctctt tttcgggtta tctgacgcat
    15241 cgcttaaccg gcgagtttaa agacaatatc gccaactatt ttggtcagtg gccggtggat
    15301 tataagagct gggcatggag cgaagatgct gcggtaatgg agaagtttaa tatcccccgt
    15361 catatgctgt ttgatgtgca aatgccaggc actgttcttg gacagatcac accacaagcc
    15421 gcgctggcga cacatttccc ggcagggctg ccggttgttt gtaccaccag tgataaaccg
    15481 gtagaagctc tgggagccgg attactggat gatgaaacgg cggtgatttc actcggtact
    15541 tatatcgcgc tgatgatgaa cggcaaagcc ttgccgaaag atccggtggc gtactggccg
    15601 attatgtctt ctattccgca aacattgctg tatgaaggtt acggtattcg caaaggcatg
    15661 tggacggtga gctggctgcg cgacatgtta ggcgagtcgt taattcagga tgccagggcg
    15721 caggatcttt caccggaaga tttactcaac aaaaaagctt cttgtgtgcc acctggctgt
    15781 aatgggctga tgacggtgct ggactggctg accaatccgt gggaaccgta caaacgcggg
    15841 attatgatcg gctttgattc cagcatggat tacgcatgga tatatcgttc gatactggaa
    15901 agcgtggcgc tgacgctgaa gaacaattac gacaatatgt gtaatgaaat gaatcacttt
    15961 gcgaagcatg tgatcattac tggtggcggt tcgaacagcg atctgtttat gcagattttt
    16021 gccgacgtgt tcaaccttcc ggcacgacgt aacgccatta acggttgtgc aagtctgggg
    16081 gcagcgatca atacggcggt aggtctgggg ctatacccgg attacgcaac ggctgtcgac
    16141 aaaatggttc gcgtgaaaga tatctttata ccgattgaga gcaatgccaa acgctacgac
    16201 gcgatgaata aaggtatttt caaagcccta accaaacata ctgatgtgat cctgaaaaaa
    16261 tcgtatgaag tgatgcatgg ggaattgggg aatgcggatt cgatccagag ctggtcgaat
    16321 gcgtaagtga ggaagaccgg gcgggaaact gcccggcctg aacatacctg aatggttatc
    16381 cccgctggcg cggggaacat aagttcgaca acttaaaaaa tgataacaag tccggtttat
    16441 ccccgctgat gcagggaaca taataaaaac tttaactagt tcggtttatc cctgttttta
    16501 caaggaatat taactacact cgatgtcttt agaataaaag acaatacgaa tcgcacttta
    16561 tca
//
LOCUS       DAZKIP010000097        16561 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZKIP010000097 DAZKIP010000000
VERSION     DAZKIP010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.098"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            276..1175
                     /gene="yegS"
                     /locus_tag="ACHOUK_003429"
     CDS             276..1175
                     /gene="yegS"
                     /locus_tag="ACHOUK_003429"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFP9898808.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(1257..2030)
                     /locus_tag="ACHOUK_003430"
     CDS             complement(1257..2030)
                     /locus_tag="ACHOUK_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9898809.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSDVDKLALQKTRVKLITV"
     gene            complement(2136..3176)
                     /gene="gatD"
                     /locus_tag="ACHOUK_003431"
     CDS             complement(2136..3176)
                     /gene="gatD"
                     /locus_tag="ACHOUK_003431"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFP9898810.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(3224..4579)
                     /locus_tag="ACHOUK_003432"
     CDS             complement(3224..4579)
                     /locus_tag="ACHOUK_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFP9898811.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            complement(4583..4867)
                     /gene="gatB"
                     /locus_tag="ACHOUK_003433"
     CDS             complement(4583..4867)
                     /gene="gatB"
                     /locus_tag="ACHOUK_003433"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFP9898812.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARGDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(4898..5350)
                     /gene="gatA"
                     /locus_tag="ACHOUK_003434"
     CDS             complement(4898..5350)
                     /gene="gatA"
                     /locus_tag="ACHOUK_003434"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFP9898813.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(5360..6622)
                     /gene="gatZ"
                     /locus_tag="ACHOUK_003435"
     CDS             complement(5360..6622)
                     /gene="gatZ"
                     /locus_tag="ACHOUK_003435"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFP9898814.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFAIADKVGFARERIILGGDHLGPNCWQQENVDAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(6651..7505)
                     /gene="gatY"
                     /locus_tag="ACHOUK_003436"
     CDS             complement(6651..7505)
                     /gene="gatY"
                     /locus_tag="ACHOUK_003436"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFP9898815.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKHYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(7804..8856)
                     /gene="fbaB"
                     /locus_tag="ACHOUK_003437"
     CDS             complement(7804..8856)
                     /gene="fbaB"
                     /locus_tag="ACHOUK_003437"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFP9898816.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            9113..10390
                     /locus_tag="ACHOUK_003438"
     CDS             9113..10390
                     /locus_tag="ACHOUK_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFP9898817.1"
                     /translation="MKTTTKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFTGAALMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            10387..11391
                     /locus_tag="ACHOUK_003439"
     CDS             10387..11391
                     /locus_tag="ACHOUK_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFP9898818.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPNLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFINDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRTTTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGINAIDPALKAELDAVNQLDFNRYATALAKYRQQREAA
                     "
     gene            11388..12353
                     /locus_tag="ACHOUK_003440"
     CDS             11388..12353
                     /locus_tag="ACHOUK_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFP9898819.1"
                     /translation="MSGARLHTLLPELTTRQPVMVIGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPEALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFATPLIIRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(12327..13073)
                     /locus_tag="ACHOUK_003441"
     CDS             complement(12327..13073)
                     /locus_tag="ACHOUK_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9898820.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFSLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(13125..13943)
                     /locus_tag="ACHOUK_003442"
     CDS             complement(13125..13943)
                     /locus_tag="ACHOUK_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFP9898821.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIHIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPVIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGSVDFNVFNGTEEELQAFVDGIKETP"
     gene            complement(14008..14808)
                     /gene="thiD"
                     /locus_tag="ACHOUK_003443"
     CDS             complement(14008..14808)
                     /gene="thiD"
                     /locus_tag="ACHOUK_003443"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFP9898822.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRALLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(14805..15593)
                     /gene="thiM"
                     /locus_tag="ACHOUK_003444"
     CDS             complement(14805..15593)
                     /gene="thiM"
                     /locus_tag="ACHOUK_003444"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFP9898823.1"
                     /translation="MQVDLLSSAQSAHTLHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGVANGGRGVDTTDAAVNAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDML
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(15628..15724)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            15927..16166
                     /locus_tag="ACHOUK_003445"
     CDS             15927..16166
                     /locus_tag="ACHOUK_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor"
                     /protein_id="HFP9898824.1"
                     /translation="MTETQIYENIKQAISSAPRNSQTMEMHLQMIKYADHLKNVTAKE
                     FCEGVGLKASFATEFSKMRNLTERLKAAGLDTTKL"
BASE COUNT         4133 a         4279 c         3975 g         4174 t
ORIGIN      
        1 acatggaggc ccttccgggt aactccatgg aggctttaat atattatatt gaagaagcca
       61 ctgtttagtt gaatattagg tatatgctct tttttgaaat ttatcggtgg ctgccgttag
      121 tattcgctgt ccccattgca agctcctggt ggtaaccact gaatcctcca tacttgaact
      181 gactatttat cctccgactt tcatcctgtt ctgactccac cttttctttt ctgctctaca
      241 ctatctatag accaatcata aaggcacata cgatcatggc agaatttccc gccagcttac
      301 tgattcttaa tggcaaaagt actgacaatc tacccttgcg cgaagcaatt atgctgttgc
      361 gtgaggaagg aatgacgatc catgtgcggg tcacctggga gaaaggcgat gccgcacgat
      421 atgtagagga ggcccggaag ttgggcgtcg caacggtgat tgccggtggt ggcgatggca
      481 ccattaatga agtttctacg gcgttgattc agtgtgaggg ggatgacata cccgcgctgg
      541 gaattttgcc attaggaacc gccaatgatt ttgccaccag tgtagggatt cctgaggcac
      601 tggataaggc gctgaaactg gcaattgccg gtaacgccat tgcgatagat atggcgcagg
      661 tcaacaaaca aacctgtttt attaatatgg cgacaggcgg atttgggacg cgtattacca
      721 cagaaacgcc ggaaaaatta aaagccgcgc tgggtggcgt ctcttacatc attcatggct
      781 taatgcgcat ggatactctg caaccggacc gttgtgaaat ccgcggtgaa aactttcact
      841 ggcaaggtga cgccctggtc attggtattg gtaacgggcg tcaggccggt ggcggtcaac
      901 aattgtgccc gaacgcgtta attaacgatg gcttgctgca actgcgcatt tttaccggcg
      961 atgaaattct tccggctctc gtatcaacct taaaatctga cgaagataac ccgaatatta
     1021 tcgaaggcgc ttcgtcgtgg tttgatatac aagccccaca cgaaatcact tttaatcttg
     1081 atggcgaacc gttgagtggg caaaacttcc atattgaaat acttccggcg gcgttgcgtt
     1141 gtcgattacc accagattgt ccattattgc gttaatcaaa ctattctctc ttgaaccagg
     1201 gagaatccgt aatgccggat gcggtgtaaa caccttatcc ggcatacaga acaatactac
     1261 accgtaatta attttacccg tgtcttctgc aatgccagtt tatccacatc tgacacggca
     1321 tcatcggtaa taataatgtc aattttttcg attggcagta cctgattaaa accgcgacga
     1381 ttaaatttcg atgaatcgag aacggcaatc actttatttg cggcggtgac catcgccccg
     1441 ctaatcgaat aaccttcatt aaaggtcgta ataccattaa ccgcatcaat accatcagca
     1501 ccgacaaaca ttaaatcggc attaatatcc tgcaatgaac gctcggcaat ggaaccatgc
     1561 atcgagcgcg ttttatggcg gaccgtgcca ccacagacga ccagagtaat gtctttattt
     1621 tcggaaaggg caaacgctgc cgggagactg ttggtaatca ccgtgatatt tttggcggtc
     1681 attaatcctt cggcgataag cattgtcgta cttccgctgt cgagaataac ggtcatccct
     1741 tcgtggatca ttttgactgc cgcctgcgct attctgtttt ttggcgcgct ggcaagctga
     1801 aatcgctctt taaacccgac ttcctgggtc tcggtttcac tattaccaga cattattttc
     1861 gccgcaccgc catgaaagcg cgtaaccacg cctttttgtt cgagaaagcg caaatcggca
     1921 cggattgtcg cttccgaggc agcaaatact cccgccagat cctgaacaag cacggttccc
     1981 tgttcattca ctaattggat gatcttattc cttcgctcga atgaattcat cgtcatttac
     2041 ccatattcaa ttgtggctag tgtaaacgaa gggggaccat tggtgaatga tctgttttgc
     2101 acaataccgg gccagcatca cgctggcccg cggtttcagg gaatgagcaa cactttgccc
     2161 ggcatagcat tacgagcgat gtcacgcacc gcctgggcga agctttcaaa gcttccacgg
     2221 tgagcgatta atggctccag gcttaactta cgttctgtca gcaaccggct cgccgtttcc
     2281 cactcctgcc ccggccaagg gctggagtag ttcatccaac tgccgataac cgtcagctct
     2341 ttacgcaata ttttgccaaa cgttgccgat gttaaatgca gatcctgatg caacgtgccc
     2401 accagtgcca gttgggcatg aggaccggca atctctaccg ccagttcgac agtttgcggt
     2461 acgccagccg tctcgaggat aagctgatta aagcgcagtt cgcgtaaaac gctctgcatt
     2521 tgcggcgcgc tcatttcgct actgttaaat gtttgcatcg caccgaaaga ttttgccagt
     2581 gccagttttt ctgagctaat gtcgatagcc gtcacactct ttgctcccag cgcgacagcg
     2641 cactgaatag ccagcaggcc aatggttccg gcaccaataa taataacgtt tttattctca
     2701 caaccttgcg ctaaatgaaa agcatgcagg ccaacggtaa tcggctcaat aaaagcccca
     2761 tcctcaatag gcatatccgt gggtagagca aagacatttt ttcgcttaac gacaatatat
     2821 tcggcaaatc caccatcacg ccgtgagcca ataaaatcat attttgcgca ctgggaataa
     2881 aaccctttca aacactctgg acaagtaaaa cagggtaata acggcacaca ggcaaccgca
     2941 tcgccagggt gtaaatcatc aacaccggat cctaccgcat caatatagcc gctaaattca
     3001 tggcctaacg ttattggata ataatgtgca ccatttttaa atatcctggg taaatcggaa
     3061 ccacataagc ccgagctggc aatttttacc cgcacctcat cctgatgttt aatttcagga
     3121 atgacgcttt ctgcaacgcg cacgatacca tcagtatcat tcaccactga tttcataaaa
     3181 actcctaatt gttaaggggg ataacctccc cctcaggtaa aaattattct gcgagaacga
     3241 ctttctcttg tttaataaag ccacgcgctc tacgccaggt catgaaaata ccggtaagat
     3301 aaattgcgcc gataataatg aaaccgggaa tattttgcgg ggagaaaacc tgaatcagta
     3361 accaggtaat gggagaaccg ccctgatcca ttgaagccac catacccccg gcttttaacg
     3421 ccccagcatt agccgccagt tgggtgtgca aaccaatagt ttgcgtcgcg atccacaggg
     3481 tgatgctcat aatgatgaca cccgagatta aggtgcggaa cagatttcca cgatgcaccg
     3541 cgaccgccat cgccacgaag aagccgatag tggcaagatc gccaaacggc agtacctgat
     3601 taccaggaac acaaacagca attaaaatgg tgagcggaat aaaaatcagg cttgccgata
     3661 ccaccgccgt atgccccagc agtaatgcgg gatccaggcc aatcaggaac tcctgaccgc
     3721 cgaacttcgc ctgtaaacga ctacgggcct gcttagcgat gggcgttaaa ccatccatga
     3781 tgggtttaat cacccgtggc attagcagca tcaccgccgc cgtttttacc gccagctgca
     3841 atacaccttt gacatcgtaa cccgcgagga tgccgataat cagccccatc acaaaaccca
     3901 cggtgacagg ctcgccaaac gggccaaatt tgcgctgaat atcgtcggcg ctaaatttaa
     3961 tacggttaac gcctgggatt ttctcgatga tagcatcgac cagcaccgca atcggcccca
     4021 tatacgccga cgtaccgtgc ggaatagcaa tgccttccag ctcaaagaaa tttcgggtat
     4081 cgcgggcaaa ccagtcgccg agcttataaa caaacgccgc gtgaattacc acacctgcca
     4141 tccctatcat ccatgaaccg gttgccagat gcagcaacgc gccggtgaag gtcatatgcc
     4201 agatattcca gatatcaaca tttaccaccc gcgtcatacg ggtcagtaac atcgccacgt
     4261 taaccagaat cgcaatcgga atcgccacca gcgcaatttg cgacgcccag gtcattggtg
     4321 aagagcccgg ccagccgaca tcgaccacat gcagattcag gtcgaaattt tccgccatcg
     4381 ctttcgccgc cgggccaatg gaatccagca ttaagccaat cacaaggcca atgccaacaa
     4441 agccaatccc gatatgcaga cccgctttaa agcaatcgcc tgccttcatg cctaaaattt
     4501 tagaaaaaat aatgatgaca atcggcaaca tcaccgtagg gccgagatcg agaatataac
     4561 gcatgacttc tgaaaacata ggtcacccct gtaagatagt cagaatttta ttttgtaatg
     4621 cttcgatacc gacaccagaa acaaaaggca tgccgtgaac taacggaata tcgccaaaac
     4681 tacgatcccc cctggcagtg gtgcatatca aatgcacgcc atccatatag gtttctattt
     4741 cattaacccg acactggatt aattcaacag gaatattatg actctgacac aactctttaa
     4801 tttcttccgc cgccatcgta gaggtcgcaa ccgcgcctcc gcaagcgaca ataatcttgc
     4861 gtttcataaa tagtcctttt tattgttaca gagggattta ttcgtctgaa tctaaaacat
     4921 actttgtgaa gtattccttt aactgagttt caggaagagt gattagtgtc tcaacgatat
     4981 cgggctgttg taacttgcca aataaacagc gtaaaagttt caattgctgc tgcggatttt
     5041 ccacaatcaa cgcaataacc aacgataccg ccacgtcatt atcatcatcc gcttgctgaa
     5101 aatgaacttt atttgttggc cttaacagat aaatggctga cgacttagca tgaatcgcct
     5161 cacaatgcgg tattgcaatg gcgtgctgct caagcattat cccggtaggg aattctgctt
     5221 ctctggcaat taatgcctgt ggccaggtat catgaaccac acctttggcg agcatctcat
     5281 taccgatatg ggttaaaact tcgctacgat cgacaaaaga aattccgcta cgaacaaaca
     5341 ggttagtcat ataccgtcct tattccgcac agccgtagcg ataggcgcgc aaaacatcat
     5401 aaattttatc cataatcagc tgatgcggta ttgctgataa ttccccggac tgaatgcgtt
     5461 caaattgttt gggaagatac tgactaatca tgcccagtgg gatgtccacg ccttgcagat
     5521 tcaccatcat cgtttcaacg ctatttttaa tccgactatg cggccagtaa taacgaatac
     5581 gatccgacag gctgtaacga atatccagta atgaatcgtt aaaacccgtg cgataatatt
     5641 ttttccagta ttgcggttcg tcgagcatca cttcttcaat taccgccagg caaccgctgc
     5701 gattttcggg ggcgataagt tcctgctcaa tttgcgccag cgcaaatatc gcctcacgta
     5761 aagcaaaggt taatgcggga ccgactttca atattgcaaa gtgatcgcgg actaattccc
     5821 aataagccgt ccgggtctgg taatcggtag aatgtgcttc ataaaccatt cgggtatttt
     5881 ctatccattg cgccagcgcc tgcgcttcct gcggctgata atggataata ttgctgtgat
     5941 caaattccac acccggctgc accacgatgg caatcacgcg tgttaatgcc tctgtcagcc
     6001 cacgggcaat aaaggccttt tgatgcgtac gtaaagtatt ggcggcatct tcaacatggg
     6061 tgatgtgtac tgactgaatg gcgctggcct caccgcccgg aaccggaact tcggtgccaa
     6121 tgacatagct cagttgctca cgctggcaat ctgtcgccac actttccgca gcaaagcaaa
     6181 gcacagcagc tcgttccgca accgtttctg gtgctaacgg tatgggatcc cccgcgcagg
     6241 acattgacgc atcaagatga attttactga agccagcacg aacatatgcc tttaccagct
     6301 cgacggattt ttccatcgcc gcatccacat tttcttgctg ccagcagttt ggccccagat
     6361 ggtcaccgcc gagaataata cgctcgcgtg caaatccgac tttatcggca atcgcaaaaa
     6421 caaattcgcg aaagtctgcc ggtgtcattc cggtataacc gccaaattga ttgacctggt
     6481 ttgacgttgc ttcaatcagc actttgcgcg tgctgttgcg atcaaatgcc agcgccgctt
     6541 cgataaccaa cggatgggca gaacagactg aacatatgcc gatatgttca ccagctttat
     6601 gccgggcaat taacgttttc atgttttttc cttgttaaat ggcaggtgcg ttatgccctg
     6661 ccctcgcagc cacaatcggc aatcactttg ctcaccacat cgcgcattgc ggatttagcc
     6721 gactgcaaat aatcccgggg atcggtcgct tcggggtgct cggtcaggta attttttaac
     6781 gcctgcgaga aggcattttt cagctccgtt gcaacgttga ttttgcatat ccccagtttg
     6841 atggtttgct gaatatcctt agtcgataac cctgacgcgc catgcagcac cagcggtaag
     6901 ttcacccact ggcgaatgtt ctccagtcga gaaaaatcaa gcgccggtgc gctggcatac
     6961 ataccatgag ccgtgccgat ggcgaccgcc agggaatcaa ttccggttgc ctcggcaaat
     7021 tcacgcgcct gagcggggtt ggtataaaaa gcatcggctt cattgacttg cacatcatct
     7081 tcctggccgc caagttgccc cagctccgct tcgacgctga catcaaagcg atggcaaaaa
     7141 tccaccacct ctttgacccg tgaaatattt tgcgcaaaag gcaaatgcga ggcgtcaatc
     7201 atgactgagc gcacgccaga acgaaccttc tgagcgatat cgtcaaattt cgtgtgatgg
     7261 tcgagatgaa tcgccagtgg atggtgataa tgcttcgcca tcgcgctgac cagcgccaac
     7321 agattttctg taccagcatg agtaaatgtg ccaggcgttc cggcgatgat gaccggcgca
     7381 tgcaggttgg cagcggtttc taccaccact tgcatcgttt cgagattgtg aatattgaat
     7441 gccggaaccg cgtaaccgcc gcgctgcgcg ttgttcagca tctgctttgt cgataccacg
     7501 tacattttca catcatgtcg attgctttcg atttcaaaac ataatgaaat catttgtttt
     7561 taaatgtcga gataacgatc acaaaaacga caatatgaaa attaatagag gaatgaaagg
     7621 caaaaaaaac ggcctcccgg agggaagccg caacaaagtg cgcgtggttt tatgcaggat
     7681 gcggcgtaaa catatgtggc agcgctgcgt aggcatgata agacgcttca gcgtcgcatc
     7741 aggcataggt tgccggatgc ggcgtaaacg ccttatccgt cctacggggt agtgccgttc
     7801 gtatcaggcg atagtaattt tgctatcgag ataaacgtcc tgcacggcgt taatcagttt
     7861 cacgccgtca gccatcgatt tcttgaacgc cttacgtcca agaatcagcc ccattccgcc
     7921 agcgcgtttg ttgataaccg cagtacgcac tgcatcactg aggtcagttt caccgcctgc
     7981 agcaccaccg gagtttatca acccggcccg gcccatatag cagttagcta actgataacg
     8041 caccagatca atcgggtttt cgctggttaa cttgctgtac acgcgatcgt cggtataacc
     8101 gtaattaatt gctttatagc cgccgttatt ttccgccatt ttttgtttga cgatatctgc
     8161 acctatggtt gccgccagat ggttcgcctg accagtcagg tcggcggaga catggtaatc
     8221 aacgccatct ttcttaaagg cggagttacg caaataggcc cacagcactg tcaccatgcc
     8281 cagctcgtgc gcacgttcaa aagccgcaga aatttcttca atctggcgac gtgactcttc
     8341 cgagccaaaa tagatagtcg caccaaccgc caccgcgccc atgttgaagg cctgctccac
     8401 gctggcatac agcgtttgat cgtaggtatt cgggtaactt agcgtctcgt tgtgattaag
     8461 ttttacgaga aatggaatgc gatgcgcata gcgccgcgat accgacgcca acacgccgta
     8521 agttgatgcc acacagttac agcccgcttc gatcgccagt tcaacaatgt ttttcgggtc
     8581 aaagtagagc gggttagcag caaatgaagc tccggcagag tgctcaacgc cctggtcaac
     8641 cggcagaata gaaagatatc ctgtgccagc cagacgcccg gtgttgtaca acgtctgcat
     8701 attacgtaac accgctggcg ggcgattatt gtcaatcatc acgcggtcta cgtagtcatg
     8761 tccggggaga taaagctggt cagaaggaat agtcatacaa cggtgctgta aaaggttgtc
     8821 ggcgtctttg ccaagcaact gcgcaatatc tgtcattact atgctcccgt aaattccgat
     8881 tggatatcgg ctatggattg tcctggcccg ccttttgcgg gcaatcataa tcctggtcgt
     8941 tacgggcgag tttttccatc ttttggatgt tttttcagcg ttttctgttg gctcgattca
     9001 tcagaaaaaa tgttagcgcg gtcaagtttt caccgcaaag gtatttaaaa ggtattatta
     9061 agtggtattg tcatcgcgta ccttacatta cctgtcatga aggaattaaa agatgaaaac
     9121 aacaacaaag ctgtcgttca tgatgtttgt tgaatggttt atctggggcg cgtggtttgt
     9181 gccattgtgg ctgtggttaa gtaaaagcgg ttttagtgcc ggagaaattg gctggtcgta
     9241 tgcctgcaca gccattgcgg cgatcctgtc gccaattctg gttggctcca tcactgaccg
     9301 ctttttctcg gcgcagaaag tgctggcggt attgatgttc actggtgcgg cgctgatgta
     9361 tttcgctgcg caacagacca cttttgccgg gttcttcccg ttactgctgg cctactcgct
     9421 aacctatatg ccgaccattg cgctgactaa cagcatcgct tttgccaacg tgccggatgt
     9481 ggagcgtgat ttcccgcgca ttcgtgtgat gggcactatc ggctggattg cctctggtct
     9541 ggcatgtggt ttcttgccgc aaatgctggg gtatgccgat atctcaccga ctaacatccc
     9601 gctgctgatt accgccggaa gttctgctct gctcggtgtg tttgcgtttt tcctgcccga
     9661 cacgccgcca aaaagcaccg gcaaaatgga cattaaagtc atgctcggcc tggatgcgct
     9721 gatcctgctg cgcgataaaa acttcctcgt ctttttcttc tgttcattcc tgtttgcgat
     9781 gccactggcg ttctattaca tctttgccaa cggttatctg accgaagttg gcatgaaaaa
     9841 cgccaccggc tggatgacgc tcggccagtt ctctgaaata ttctttatgc tggcattgcc
     9901 gtttttcacc aaacgctttg gtatcaaaaa ggtattattg cttggtctgg tcaccgctgc
     9961 gatccgctat ggcttcttta tttacggtag tgcggatgaa tatttcacct acgcgttact
    10021 gttcctcggc attttgctgc acggcgtaag ttacgatttt tactacgtta ccgcttacat
    10081 ctatgtcgat aaaaaagccc ccgtgcatat gcgtaccgct gcgcagggac tgatcacgct
    10141 ctgctgccag ggcttcggca gtttgctcgg ctatcgtctt ggcggtgtga tgatggaaaa
    10201 gatgttcgct tatcaggaac cggtaaacgg actgactttc aactggtccg ggatgtggac
    10261 ttttggcgcg gtgatgattg ccattatcgc cgtgctgttc atgatctttt tccgcgaatc
    10321 cgacaacgaa attacggcta tcaaggtcga tgatcgcgat attgcgttga cacaagggga
    10381 agttaaatga aaacagaacg tattctcggt gctctttatg ggcaggcgtt aggggatgcg
    10441 atggggatgc cctccgagct ttggccacgc agccgcgtta aagcacactt tggctggatt
    10501 gaccgttttc tgcctggacc aaaggagaac aacgcggcct gttattttaa ccgcgccgaa
    10561 ttcaccgacg atacctcgat ggcgctgtgt ctggcggatg cgttactgga acgtgaaggc
    10621 aagatcgacc cgaatctgat tgggcgtaat attctcgact gggcgctgcg tttcgacgcc
    10681 tttaacaaaa acgtactagg tccgacctcg aagattgcgc ttaacgccat tcgcgacggt
    10741 aaacccgttg ctgaactgga aaacaacggc gtgaccaacg gcgcagcgat gcgcgtctcg
    10801 ccattaggtt gtttgcttcc ggcgcgtgat gttgattcct ttattaatga tgtggcgctg
    10861 gcgtcgagcc cgacccataa atccgatctg gcggttgcgg gcgcggtagt catcgcatgg
    10921 gcgatttctc gtgctattga cggagaaagc tggtcagcga ttgttgattc attgccttca
    10981 attgcgcgac atgcacagca aaaacgcacc accacgttca gcgcctcact ggcagcgcgg
    11041 ctggagattg cgctgaaaat tgtgcgcaat gccgacggca ctgaatccgc cagcgaacag
    11101 ctttaccagg tcgttggcgc aggtaccagc actattgagt ccgttccgtg cgccattgcg
    11161 ctggttgaac tggcacaaac cgacccgaat cgctgcgccg tcctgtgcgc taaccttggc
    11221 ggcgatacag acaccatcgg tgctatggcg acggcaatct gcggcgcgtt gcatggcatt
    11281 aacgctatcg atcctgcgtt aaaggcggag ttggatgcgg taaatcagct tgatttcaac
    11341 cgctatgcca cagcgctggc gaaatatcgt caacaacggg aggcggcatg agcggcgctc
    11401 gattacacac gctgctgcct gaattaacca cgcgtcagcc ggttatggtg attggcgcgg
    11461 cagtcattga tgtgatcgcc gacgcttatg ccctcccctg gcgtgggtgc gatatcgaac
    11521 tgaaacagca gagcgttaac gttggcggct gcgcactgaa tattgccgtg gcgttaaagc
    11581 gcctcggcat cgaagcgggt aacgccttgc cgctcggtca gggcgtgtgg gcggagatta
    11641 ttcgcaaccg gatggcaaaa gagggcttaa tcagcctgat cgataacgcc gaaggtgata
    11701 acggctggtg tctggcgctg gttgagccgg atggcgaacg cacttttatg tcattcagcg
    11761 gtgtggaaaa tcagtggaat cgccagtggc tggcgcgatt aaccgttgcg cctggcagcc
    11821 tgctctattt ttccggttat caactggcct cgccctgcgg cgaattatta gtggaatggc
    11881 tggaaaagct gcaagacgtg acgccgttta tcgattttgg cccacgtatt ggcgatatcc
    11941 cggaggcatt actggcgcgg atcatggcct gtcgaccttt agtgtcgctc aatcgtcagg
    12001 aggctgagat tgccgccgaa cgttttgctt tatccgcaga gataacaaca cttggcaagc
    12061 aatggcagga gaaatttgcc acgccgttga tcattcgtct cgataaagag ggcgcatggt
    12121 atttcagcaa cgacgcttct ggctgcattc cggcatttcc cacgcaagtt gtagacacca
    12181 ttggggcggg cgacagtcat gcaggtggcg tacttgccgg gttggcctct ggtctgccac
    12241 tggcggatgc cgtattactg ggcaatgcag tggcgtcgtg ggttgtcggg catcggggcg
    12301 gtgattgtgc gccaacgcgc gaggaactac tcctcgcaca caaaaacgta tagatcgctg
    12361 cgacagtggc taatgctgta ctcaataggc cgttgctgtt ggtcaagcgc cacttgcttg
    12421 atcaccagca ccggtatttt gctatctaac tgaatatgtg actgaaactc ggcatctggc
    12481 atccgggcgc taacgcggga acgtgtacgt tgtgggtaaa tgtgctggct gcggaaatag
    12541 tcataaagag aaattccgat ggcatcaaca tcatgaatta aatgcgccgg aacccacgat
    12601 tcctcaatcg ataccgcttc ttcatcgaca tagcgaatac gcttaagcga aaagacatca
    12661 cttcccgctt cgaccgccag ctgctgcgcg acttcttcgg gacatttcac gacacgcttg
    12721 ttaacccata gcgtatcggg ctttttcccg cgcaatacca cctgctgaga aaaacctcgc
    12781 gcttctttca acgagtattc gaagatgttg ttgatctgcg tgccgtaacc gcgcgaacgc
    12841 gtcaccacac cctcttcttc cagcgcctgc atcgccttgc gcaccgtaat gcgtgaaacg
    12901 ccggttaact gacttaaatc acgctcaccg ggcaaaatat tgccatgctc cagcaccccg
    12961 ctgcgcacgg cattttttac cgtttcggca aacttaatat aaagcggcgt gttatccgcc
    13021 gctaaaatac gctcgttcag ttgggcaatc agctgggtat gcgcttgttc catttgtctt
    13081 tttcctgcca gggagtctgg tgggagtata tcactgcttc ttatttaagg cgtttcttta
    13141 atcccatcaa cgaatgcctg caactcctcc tctgtgccat taaacacatt aaaatccacc
    13201 gaaccattaa tgccatctac ctgcccacgg tcggaatgct gccagaaacg ccaggccatg
    13261 ccgtcattgt ccgggcgacg ttgatagtag tgagccaccc accacggata ttcattgaaa
    13321 tagcccgcca gattggtgtg ataaaaaacg gctcctgagt aaataaccgg cttttttccc
    13381 gtactttttt cgaccatttt tagccactga cttacccgct tgcgtaattc ttttgccgat
    13441 aattttcccc gttcttctac gtccagcacg gcagggaaat cgccttgtga gaaatccacc
    13501 gtttgcagaa ataatcgcgc ctgaacaggc gctgctaccg acggggagaa ataatgatac
    13561 gccccgcgca gcaggccatt ttcgcggctt agttgccagt tacgcgaaaa ataggggtcc
    13621 accagctttt cgccttccgt cgccttgata aaggcaaatt gtaagcggat accgttatcg
    13681 cgcatttttg ccacccgctg ccagtcgatc cgctcctgcc agcgcgaaac atctattccg
    13741 tgaatggtgt aactggcagg aatatgaata gcgaaagatt taaccgggcg ataaccgtag
    13801 aagtggatgt agtctgtaat ctgaactgct gtcgaataga aaaaattcac cgtctgacgc
    13861 ggataaattg caacgatgga aattagccca agagcgcata atagagcggt gagttttttt
    13921 cgactggtaa ttcttagctg cattggtttc atccctgaat gtcagtgcca aaggctgaca
    13981 ataaccaaag cagctatagt acggtgctca ccaccaggcg tggaagtggt gaaccggacc
    14041 aataccgtga ccaacttcca gcgtgtcggc ctgggctaac gccgatgaaa gccagctttt
    14101 tgcctcctgt acggtgtcag cccagtttgt atggcgcggg cgtagtgcag ccaacgccgc
    14161 agagagtgta caaccagtgc cgtgggtgtt tttggtcata atgcgcggtg cggtaaaccg
    14221 ttgttcaccc tcgcgggtaa acagccagtc cgggctttgc tcatcatcca gatggccacc
    14281 tttcattagc actgcgccac agcccatcgc caacagcgct cgcccttgtt ccagcatttc
    14341 ctgttcggtg cgcgcgtgtg gcgcgtcgag caaggcagca gcttcgggca agtttggcgt
    14401 tattaatgaa acctgtggca ataatcgact gcgtagcgtg gcaactgccg aaggtgaaag
    14461 cagtgggtcg ccactttttg ccagcataac ggtgtcgagt accacgtttt ggatctgata
    14521 acgttgcaac cgttctgcca ccgcttcaac aatatcggtt tccgccaaca taccgatttt
    14581 agtggtatcg attcgcacat cgctgaacac cgaatcgagc tgggcggcga caaaatcagg
    14641 ctcaatgcga tacaccgact gtacgccacg ggtattttgc gccaccagtg cagtaataac
    14701 tgagcaacca taagcgccaa gtgccgagaa ggttttaaga tcggcctgaa tccccgcacc
    14761 accactcgga tcagtaccgg caatcgtcag agcgttaatt cgtttcatgc ctgcacctcc
    14821 tgcgtcaatt gccagagcgc atcaaggaaa tgtggaacaa aactgcctgg cccctcgctt
    14881 ctggcgactg cgcgttctcc ggcttgtttc atccagtgac aggcagatgc gacattttcc
    14941 agcatatcgc ctggtaacgc acaacaggca gcgacaaccg ccgataatgc acagccagtt
    15001 cctaccactt tggtcattaa cggatcgcca ccgtgaatgc caacggcgcg atggccatcg
    15061 gtaacataat ccacctcgcc agtgaccacg acgattgcgc cagtttcccg tgccagtgtt
    15121 tgtgcagcgg gtatcgcgtt aactgcggcg tcagtggtat ccactccccg tccaccatta
    15181 gcaacgccag ctaatgccat gatttccgaa gcattaccac gtatcgctgc cggtttaaaa
    15241 gataaaagtt catgacaaaa atggcggcga taatcgagcg caccaaccgc tactggatca
    15301 agcgtccagg gtgtttgaga gctttttgct tgctcaacgg cagcacgcat cgcctgtgcg
    15361 cgtggctgcg tcagtgtgcc aacgttaatc aacaaggcac tggcgatagc cgcaaactga
    15421 ctggcctctt cggtttcgat aaccatcgct ggcgatgcac cgagcgccag caaggtattg
    15481 gcagtaaagg tttgcaccac atcgttggtc atgcagtgca caagagggga atgttggtga
    15541 aaaaggtgta acgtgtgcgc agattgcgcg gaactcagca ggtcgacttg catagtttgc
    15601 tcctgccata acgtgaagaa gcaatgacct ggtggtccgt gacttcccta cgctggcatt
    15661 atccagatca ggtgatacgg gtatttctca gccttcacgc agaagggcac cccgagtcgt
    15721 ttggttgaat ctctatattt tgtgccagct taggggaaga ggcttgcaga tgcaagctcg
    15781 cctccattca ttacgtcttc actgtgtacc ccatatcagt aatgaaaaag aaacaattca
    15841 cacccatcat tcaggtggta aaaactgtag actatcttaa ttagctaatt tatattattt
    15901 atctaaattt gataggtgag ataaaaatga cagaaaccca gatctacgag aacattaagc
    15961 aagccattag cagcgcacca cgaaatagcc aaacgatgga aatgcacctc cagatgatca
    16021 aatacgctga ccatctgaaa aacgtcacgg caaaagagtt ttgcgaaggt gtaggactta
    16081 aagccagctt tgctacagag ttcagcaaaa tgagaaatct taccgagcga ctcaaagccg
    16141 ccggacttga taccacgaag ttataacaaa cgaaatcatc aatatattac tttcgttttc
    16201 cagggttccc tgtatatgta cctatgatca taaaaaccag ttaccaccac gtagctgata
    16261 ccataatatt ttcattttta ctttaaataa atagataaat ttagagagtg gaataattaa
    16321 ggtctatttt ctctagcttt ttgagattga tagttgagaa tgataattct ttcatctcaa
    16381 taacaccgaa gtttgatagc tgtttaagcc acagttgaag accagcatca gagcaacctg
    16441 ctcgatatgt tacatcacta cattttatta gtgttgattt cctcttatag agataaataa
    16501 ggacttcata gatgcgtttt ccggtgtcat tggatacgcg ctataccccc attcttggct
    16561 a
//
LOCUS       DAZKIP010000098        16339 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZKIP010000098 DAZKIP010000000
VERSION     DAZKIP010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.099"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            78..2774
                     /gene="stcE"
                     /locus_tag="ACHOUK_003446"
     CDS             78..2774
                     /gene="stcE"
                     /locus_tag="ACHOUK_003446"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M66 family glycomucinase StcE"
                     /protein_id="HFP9898825.1"
                     /translation="MNTKMNERWRTPMKLKYLSCTILAPLAIGVFSATAADNNSAIYF
                     NTSQPINDLQGSLAAEVKFAQSQILPAHPKEGDSQPHLTSLRKSLLLVRPVKADDKTP
                     VQVEARDDNNKILGTLTLYPPSSLPDTIYHLDGVPEGGIDFTPHNGTKKIINTVAEVN
                     KLSDASGSSIHSHLTNNALVEIHTANGRWVRDIYLPQGPDLEGKMVRFVSSAGYSSTV
                     FYGDRKVTLSVGNTLLFKYVNGQWFRSGELENNRITYAQHIWSAELPAHWIVPGLNLV
                     IKQGNLSGRLNDIKIGAPGELLLHTIDIGMLTTPRDRFDFAKDKEAHREYFQTIPVSR
                     MIVNNYAPLHLKEVMLPTGELLTDMDPGNGGWHSGTMRQRIGKELVSHGIDNANYGLN
                     STAGLGENSHPYVVAQLAAHNSRGNYANGIQVHGGSGGGGIVTLDSTLGNEFSHEVGH
                     NYGLGHYVDGFKGSVHRSAENNNSTWGWDGDKKRFIPNFYPSQTNEKSCLNNQCQEPF
                     DGHKFGFDAMAGGSPFSAANRFTMYTPNSSAIIQRFFENKAVFDSRSSTGFSKWNADT
                     QEMEPYEHTIDRAEQITASVNELSESKMAELMAEYAVVKVHMWNGNWTRNIYIPTASA
                     DNRGSILTINHEAGYNSYLFINGDEKVVSQGYKKSFVSDGQFWKERDVVDTREARKPE
                     QFGVPVTTLVGYYDPEGTLSSYIYPAMYGAYGFTYSDDSQNLSDNDCQLQVDTKEGQL
                     RFRLANHRANNTVMNKFHINVPTESQPTQATLVCNNKILDTKSLTPAPEGLTYTVNGQ
                     ALPAKENEGCIVSVNSGKRYCLPVGQRSGYSLPDWIVGQEVYVDSGAKAKVLLSDWDN
                     LSYNRIGEFVGNVNPADMKKVKAWNGQYLDFSKPRSMRVVYK"
     gene            2861..3736
                     /gene="gspC"
                     /locus_tag="ACHOUK_003447"
     CDS             2861..3736
                     /gene="gspC"
                     /locus_tag="ACHOUK_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFP9898826.1"
                     /translation="MLFFLSFRGDRGLFIKDIVLKMLTPNRLLCVILLIAGYQLVSVI
                     HHFWLTQAASVPGLSRVSAPETAVTGDQTEERFVFTLFGRASPLSSEGRAQETMPSLS
                     DDLLSGEDLDVRGILYSSVAEHSVAIFAHNNRQFSLSVGEKVPSYDATISAIFSDHIV
                     INYQGKTVSLPLRYDNTEKKNAYDNNNLTVGDVITQDNFRVESVFDIMSFSAVTVNNT
                     LSGYRLIPGKHSSLFYNAGLHDNDLAVSVNGSELRDTRQAQQIMKQLPELKEIKITVE
                     RDGQLYDAFIAVGEN"
     gene            3737..5704
                     /gene="etpD"
                     /locus_tag="ACHOUK_003448"
     CDS             3737..5704
                     /gene="etpD"
                     /locus_tag="ACHOUK_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="variant type II secretion system secretin EtpD"
                     /protein_id="HFP9898827.1"
                     /translation="MFRKWLNGRLPVLVFTTVILGAIPGWGAEFSANFKDTDIQEFIN
                     TVSKNLHKTVIINPDVQGTITVRSYDMLNEEQYYQFFLSVLDVYGFAVVDMHNGILKV
                     VRSKDAKTSAVPVASDVSPGTGDEVVTRVVPVSNVAARDLAPLLRQLNDNAGAGSVVH
                     YEPSNVLLMTGRAAVMKRLMEIVERVDKVGNRSVATVPLTYASATDVARLVTELTKET
                     DKTAIPAWMTAKLVADERTNSVLVSGEPISQQRIISIIKQLDRQEDVQGNTKVIYLKY
                     AKAKDLVEVLTGISSSIENDSKKSPSTEALRKGVTIKSHEQTNALILTGAPDVIRDLE
                     NVISQLDIRRPQVLVEAIIAEIQDADGLNLGIQWVNKHAGVAQFTSTGLPITTMVQTR
                     QNEILDSDQSNALSMFNGIAAGFYQGNWAMLLTALSTSSKNDILATPSIVTLDNMEAT
                     FNVGQEVPVLSGSQTTSGDNIFNTVERKTVGIKLRVKPQINEGDSVLLEIEQEVSGVA
                     DTAVATTTDLGATFNTRTVTNAMLVGNGETVVVGGLLDKSIRGSESKVPLLGDIPVLG
                     HLFRAKSEQTAKRNLMLFIRPTIIRERDGFRHASAEKYQSFNQEQVQSRGKETTALTL
                     NEEQLRLSPDQDDTAFRKVKAAIAAFYAQEM"
     gene            5704..7209
                     /gene="gspE"
                     /locus_tag="ACHOUK_003449"
     CDS             5704..7209
                     /gene="gspE"
                     /locus_tag="ACHOUK_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFP9898828.1"
                     /translation="MSRVVQNVSESRPLLPFSFSRTQRILLLREQEGNRVFCMEDTPA
                     SALLEVRRVAEGPLNVTTVSAEAFEKQLVSSYQRDSDEARQMMAEIGNEMDFYTVAGE
                     LPDREDLLDANDDAPIIRLINAMLTEAIKEKASDIHIETYERHLQVRFRIDGVLREIL
                     RLHRNLASLLISRIKVMARLDIAEKRVPQDGRMVLRIGGRAVDVRVSTLPSNHGERIV
                     LRLLDKNSVSLDLAALGMSQQNQRHIDALIRRPHGIILVTGPTGSGKSTTLYAALSLL
                     NPRDRNIMTVEDPVEYELDGISQTQVNPKVDMTFARSLRAILRQDPDVVLVGEIRDGE
                     TAQIAVQASLTGHLVLSTLHTNSAAGALSRLQDMGIPPFLLSTSLLAVLAQRLVRTLC
                     PRCRQPCQVSTELAMDMDIPPETTIWQPAGCQHCSFTGYHGRTGIHELLLIDDRIRTA
                     IYQGEGELGITRLAGSRYLTLRGDGRQKVLAGETSWEEVVRVTESRLQEEE"
     gene            7211..8434
                     /gene="gspF"
                     /locus_tag="ACHOUK_003450"
     CDS             7211..8434
                     /gene="gspF"
                     /locus_tag="ACHOUK_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFP9898829.1"
                     /translation="MALFHYQASDIHGRKRSGILEADSARHARQLLREQALIPVRLDE
                     KQVHHKHSLRSILRFRPRGGSSAELALLTRQLATLVAASLPLEEALDALLRQSEKPRQ
                     RNLIAAVRTKVLEGHSLAAAMGMFPGTFERLYCAMVAAGETSGRLDVVLSRLADYTEQ
                     RQIMRNRLLQALLYPCVLTLVAVGVIAILLTAVVPKVVEQFIHMKQTLPLSTRVLMGA
                     AEVSQTWGPWLLLAAALGGIAGRMILHQPSQRLAFHHLLLRLPVVGRISRGLNTARYA
                     RTLSILNASAVPLLQAMHISGDVLSNDWARHQLATAAELVREGVSLHQALEQTSLFPP
                     MMRHMIASGENSGELDSMLERAADNQDREFSTQMQLALGLFEPLLVVGMAGVVLFIVL
                     AILQPLLQLNNMMNM"
     gene            8465..8899
                     /gene="gspG"
                     /locus_tag="ACHOUK_003451"
     CDS             8465..8899
                     /gene="gspG"
                     /locus_tag="ACHOUK_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFP9898830.1"
                     /translation="MRKQHQRGFTLLEIMVVIVILGVLASLVVPNLMGNKDKADRQKV
                     MSDLVALESTLDMYRLDNNRYPTTEQGLRALVSKPTVQPEPRNYRQDGYIRRLPQDPW
                     GGDYQLLNPGQYSDIDIFSPGPDGVPNTEDDIGNWTLGNAQP"
     gene            8896..9450
                     /gene="gspH"
                     /locus_tag="ACHOUK_003452"
     CDS             8896..9450
                     /gene="gspH"
                     /locus_tag="ACHOUK_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFP9898831.1"
                     /translation="MSQRGFTILEMMLILLLMGITAGLVLMSFPDSAQNHLQQQRERL
                     QAQLDYALDRSQQDGLLMGIQVRFDGWKFKVLQRGTAESPPTLAEGGDIWQGYVWQTW
                     QPRRAAMGGKLPDDVRLELQYLRGLQWMSEHDDGAEPDILLLPGGEVTPFRLLFRQVG
                     EEAVVGLQVDENGLMTLFEGEVSL"
     gene            9447..9812
                     /gene="gspI"
                     /locus_tag="ACHOUK_003453"
     CDS             9447..9812
                     /gene="gspI"
                     /locus_tag="ACHOUK_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFP9898832.1"
                     /translation="MKGQEGMTLLEVIVALVVFALAGMALMQASTQQAAGIGRMEEKV
                     LAGWLADNQMVQLQLEKTWPENGWGEKTISFAGTEWYLRWGGPDSDVPQPRSLEVEVR
                     RTKEETSALVSLRSSVVRE"
     gene            9809..10408
                     /gene="gspJ"
                     /locus_tag="ACHOUK_003454"
     CDS             9809..10408
                     /gene="gspJ"
                     /locus_tag="ACHOUK_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFP9898833.1"
                     /translation="MSQQRVKGFTLLEMLLALAVFAALSISAFQVLQGGIRAHELSRD
                     KVQRLAELQRGISQMERDLTQMLPRHSRGNEGLLLAAPHLLKSDDWGISFTRNSWLNP
                     AGMLPRPELQWVGYRLRQQKLERLSYFHVDHPSGVSPDVRVMLDGVHAFRLRFFVNGD
                     WQARWDSTGILPQAVEVTLVMDDFAELPRLFLVSKETAE"
     gene            10405..11382
                     /gene="gspK"
                     /locus_tag="ACHOUK_003455"
     CDS             10405..11382
                     /gene="gspK"
                     /locus_tag="ACHOUK_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFP9898834.1"
                     /translation="MKLREQGVALLVVLLILSLMVTIAAVIAERNGRTFLRTVAQLDQ
                     LQAKWDGYTAETIAKQILQRSRQESPRKTHLAQNWAQSERQFETRGGDVRGQIVDAQA
                     CFNLNAINYGVVDLTSIPYAARIFQQLLINLQVELLQARQVTAALRDWIDRDDKPVRG
                     GAEDEVYMGMEPPYLAANQPMQDVSELRLIRGIDARLYRKLLPYVCVLPTSDLSVNVN
                     TLLDSQAPLLAALFLTKPDSLPVTELLQRRPRTGWESVAAFLDAPPLKDIDTSAAMPV
                     LAVSSNYFLVRLHVRSGEHLFSQQTLMQWREERFRIIQRQYGLTMREVP"
     gene            11316..12593
                     /gene="gspL"
                     /locus_tag="ACHOUK_003456"
     CDS             11316..12593
                     /gene="gspL"
                     /locus_tag="ACHOUK_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFP9898835.1"
                     /translation="MEGGAFPDHSATVWINDEGGSLKKNNKHVEAGRLFLRLPEDDSE
                     PLLWRLIAPEETGAGSVAVGETDPQLVGLLSRYPAWVLVPSGRIAFHQVTLPHRTWRQ
                     RLQVLPFLLEEQLATDIEQLHFAILHQSGDRCDVAVVEKAVMHRWLAYCGQLGVREVR
                     LLPDVLMLPLATEGWSAVKLAGQWLFRRDRYAGMTVELSWLTHFLSLTAPPVIESYSV
                     PPVPCPGPKTTEWRNQPGRDLLQLVAEGDGYDGADLRQGEFARQGTWYARFRPWRYVA
                     GALLACVFLAGSNAGLAHYRLWQQAQFWRYESVRVYQQLFPSEKAVKDPRRQMLRHLQ
                     QSTGNNIPELGSVMRQLQQLLSETSTIRLQALAWDSSSQTLKIDLQAASFQALEHFQQ
                     IAGEKYLIQPGEIRQQPQGVESRLILRVNNERA"
     gene            12580..13092
                     /gene="gspM"
                     /locus_tag="ACHOUK_003457"
     CDS             12580..13092
                     /gene="gspM"
                     /locus_tag="ACHOUK_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HFP9898836.1"
                     /translation="MNELKKRLQYVSPRERRLLIGCVALLVVVFVYYVLWQPWLIRED
                     KWRTVVTREKNTVEWMKQQVPNIKRREASMPEQGEPLGLSAAVTRTSAAYGVSVTRLQ
                     PQGERLAVTLAPVEFNALMQWLTQLERRHRIRTMVFDVAAQGNPPGQVTVNRLVLSQD
                     DVNKDASLSR"
     gene            13213..13983
                     /locus_tag="ACHOUK_003458"
     CDS             13213..13983
                     /locus_tag="ACHOUK_003458"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFP9898837.1"
                     /translation="MGGVTGSFLNVVIHRLPIMLERRWRREALTLLAHSVPESGPAFN
                     LMIPQSHCPCCFHPLGIRDNIPLLSYLFLKGKSRCCGERIPVYYPLVEITNSVLFILA
                     ASRFPPGLTLAAAWLFISMLLVLAVIDCHTALLPDVLTLPLLWLGLLFSLQRGVVTLE
                     EAVVGAVSGYLCLWGLYWLFRFATGREGLGYGDFKLAAALGAWMGWQALPSILLFASV
                     SGLIVTVLLRIVTATDFTRPLPFGPWLALSGVCHFLLM"
     gene            14075..14476
                     /gene="eptO"
                     /locus_tag="ACHOUK_003459"
                     /gene_synonym="gspS"
     CDS             14075..14476
                     /gene="eptO"
                     /locus_tag="ACHOUK_003459"
                     /gene_synonym="gspS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspS family T2SS pilot lipoprotein variant EptO"
                     /protein_id="HFP9898838.1"
                     /translation="MMGNILKKLNCIASLLVLVTISGCHQSPSIHKQATVPPSEQLEQ
                     MASIVSATRYLKMRCNRSDLPDEQSILNVANRIAIGKGWQSLTQEDIRKHSDDIYVRL
                     TRDSTPEYIKCREFNRRLVPFIGELLARGRG"
     gene            14655..15826
                     /locus_tag="ACHOUK_003460"
     CDS             join(14655..14904,14904..15826)
                     /locus_tag="ACHOUK_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611522.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP9898839.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQNYTVADAAKAMDIGLSTMTRWVK
                     QLRDERQGKTPKASPITPEQIEIRELRKKLQRIEMENEIFKKGYNRALDVRLPEQFSI
                     IGKLRAHYPVVTLCHVFGVHRSSYRYWKNRPEKPDGRRAVLRSQVLELHNISHGSAGA
                     RSIATMATLRGFRMGRWLAGRLMKELGLVSCQQPAHRYKRGGREHVTIPNHLGRQFAV
                     TEPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTIKALKMAWEI
                     RSKPAGVMFHSDQGSHYTSRQFRQLLWRYQIKQSLSRRGNCWDNSPMERFFRSLKNEW
                     IPVTGYMNFSDAAHEITDYIVGYYNALRPHEYNGGLPPNESENRYWKNSKAVASFC"
     gene            complement(16147..>16339)
                     /locus_tag="ACHOUK_003461"
     CDS             complement(16147..>16339)
                     /locus_tag="ACHOUK_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9898840.1"
                     /translation="KCKEPYIKEQKLYPFKQEASKNRKLPQGESLPTKTTLAIIIYCL
                     NILTHITAKLYYNTQMIDL"
BASE COUNT         4031 a         3594 c         4630 g         4084 t
ORIGIN      
        1 tataaaaata accaacgact agtgaataag tcgctcctga aaaaataaaa tatagaaata
       61 ctgttatatc cggctgcatg aacactaaaa tgaatgagag atggagaaca ccgatgaaat
      121 taaagtatct gtcatgtacg atccttgccc ctctggcgat tggggtattt tctgcaacag
      181 ctgctgataa taattcagcc atttatttca atacctccca gcctataaat gatctgcagg
      241 gttcgttggc cgcagaggtg aaatttgcac aaagccagat tttacccgcc catcctaaag
      301 aaggggatag tcaaccacat ctgaccagcc tgcggaaaag tctgctgctt gtccgtccgg
      361 tgaaagctga tgataaaaca cctgttcagg tggaagcccg cgatgataat aataaaattc
      421 tcggtacgtt aaccctttat cctccttcat cactaccgga tacaatctac catctggatg
      481 gtgttccgga aggtggtatc gatttcacac ctcataatgg aacgaaaaag atcattaata
      541 cggtggctga agtaaacaaa ctcagtgatg ccagcgggag ttctattcat agccatctaa
      601 caaataatgc actggtggag atccatactg caaatggtcg ttgggtaaga gacatttatc
      661 tgccgcaggg acccgacctt gaaggtaaga tggttcgctt tgtttcgtct gcaggctata
      721 gttcaacggt tttttatggt gatcgaaaag tcacactctc ggtgggtaac actcttctgt
      781 tcaaatatgt aaatggtcag tggttccgct ccggtgaact ggagaataat cgaatcactt
      841 atgctcagca tatttggagt gctgaactgc ctgcgcactg gatcgtgcct ggtttaaact
      901 tggtgattaa acagggcaat ctgagcggtc gcctaaatga tatcaagatt ggagcaccgg
      961 gtgagctgtt gttgcataca attgatatcg ggatgttgac cactccccgg gatcgctttg
     1021 attttgccaa agacaaagaa gcacataggg aatatttcca gaccattcct gtaagtcgta
     1081 tgattgttaa taattatgcg cctctacacc taaaggaagt tatgttacca accggagagt
     1141 tattgacaga tatggatcca ggaaatggtg ggtggcatag tggtacaatg cgtcaaagaa
     1201 taggtaaaga attggtttcg catggcattg ataatgctaa ctatggttta aatagtaccg
     1261 caggcttagg ggagaatagt catccatatg tagttgcgca attagcggca cataatagcc
     1321 gcggtaatta tgctaatggc atccaggttc atggtggctc cggaggtggg ggaattgtta
     1381 ctttagattc cacattgggg aatgagttca gtcatgaagt tggtcataat tatggtcttg
     1441 gtcattatgt agatggtttc aagggttctg tacatcgtag tgcagaaaat aacaactcaa
     1501 cttggggatg ggatggtgat aaaaaacggt ttattcctaa cttttatccg tctcaaacaa
     1561 atgaaaagag ttgtctgaat aatcagtgtc aagaaccgtt tgatggacac aaatttggtt
     1621 ttgacgccat ggcgggaggc agccctttct ctgctgcaaa ccgtttcaca atgtatactc
     1681 cgaattcatc ggctatcatc cagcgttttt ttgaaaataa agctgtgttc gatagccgtt
     1741 cctccaccgg cttcagcaag tggaatgcag atacgcagga aatggaaccg tatgaacaca
     1801 ccattgaccg tgcggagcag attacggctt cagtcaatga gctaagtgaa agcaaaatgg
     1861 ctgagctgat ggcagagtac gctgtcgtca aagtgcatat gtggaacggt aactggacaa
     1921 gaaacatcta tatccctaca gcctccgcag ataatagagg cagtatcctg accatcaacc
     1981 atgaggccgg ttataatagt tatctgttta taaatggtga cgaaaaggtc gtttcccagg
     2041 ggtataaaaa gagctttgtt tccgatggtc agttctggaa agaacgtgat gtggttgata
     2101 ctcgtgaagc gcgtaagcca gagcagtttg gtgttcctgt gacgaccctg gtggggtatt
     2161 acgatccgga aggcacgctg tcaagctaca tctatcctgc gatgtatggt gcctatggct
     2221 tcacttattc cgatgatagt cagaatctat ccgataacga ctgccagctg caggtggata
     2281 cgaaagaagg gcagttgcga ttcagactgg ctaatcaccg ggctaacaac actgtaatga
     2341 ataagttcca tattaacgtg ccaacagaaa gtcagcccac acaggccaca ttggtttgca
     2401 ataacaagat actggatacc aaatcgctca cacctgcgcc agaaggactt acctatactg
     2461 taaatgggca ggcacttcca gcaaaagaaa acgagggatg catcgtgtcc gtgaattcag
     2521 gtaaacgtta ctgtttgccg gttggtcaac ggtcaggata tagccttcct gactggattg
     2581 ttgggcagga agtctatgtc gacagcgggg ctaaagcgaa agtgctgctt tctgactggg
     2641 ataacctgtc ctataacagg attggtgagt ttgtaggtaa tgtgaaccca gctgatatga
     2701 aaaaagttaa agcctggaac ggacagtatt tggacttcag taaacctagg tcaatgaggg
     2761 ttgtatataa ataatcctga gacggtgcgg ccacttcggc cgcattagta acacattcgt
     2821 cccgttaatc aaaacataaa gtagcgtcca gcgttttatt atgttgttct ttctatcatt
     2881 ccggggtgac cggggcttat ttataaaaga tatcgtactt aaaatgctga cgccaaaccg
     2941 gctattgtgc gtaattttac ttattgccgg atatcagctg gtgtcggtta tccatcattt
     3001 ctggctgact caggcagcat cggtgcccgg cttatcccgt gttagcgcgc cggaaacagc
     3061 ggtaactggt gatcaaactg aagaacgttt tgtttttacg ttattcggca gggcatcccc
     3121 actatcatcg gaggggagag cgcaggaaac aatgccttcc ctgtcagatg atctgctttc
     3181 aggggaggat cttgacgtga gaggtatact ttatagttcg gttgcagagc attccgttgc
     3241 catatttgca cataataaca gacagttcag tctgagcgtc ggcgaaaaag tacccagcta
     3301 cgatgctacg attagtgcta tttttagcga tcatattgtt attaactatc agggaaaaac
     3361 tgtatcactg cctctgcgat atgataatac cgaaaaaaag aatgcatatg acaataataa
     3421 tttaacagtt ggagacgtga taacccaaga taattttcgg gtagaaagtg tttttgatat
     3481 tatgagcttt tcagccgtta cggttaataa tacattaagc ggttatcgct tgattccggg
     3541 taagcacagt tcgttatttt ataatgctgg gttgcatgat aacgatctgg ccgtatcggt
     3601 taatggttca gaattgcgtg ataccagaca ggcgcagcag ataatgaagc aattgccaga
     3661 acttaaagaa ataaaaataa ccgtcgagcg tgatggtcag ttatatgatg catttattgc
     3721 tgtaggagaa aactgattgt tcaggaaatg gttgaatggg cgtttgccgg tacttgtgtt
     3781 cactacagta attttggggg ccattccagg gtggggggct gaattttcgg ccaactttaa
     3841 agatacggat attcaggagt tcataaatac tgtcagtaaa aatttacaca aaacggtaat
     3901 aattaatcct gacgtgcagg gaaccatcac tgtacgcagc tacgatatgc tgaacgagga
     3961 acaatattat cagttctttc tcagtgtgct ggacgtttat ggttttgctg tggtcgatat
     4021 gcacaacggt atactgaaag tagtgcgctc aaaagatgcc aaaacgtcgg cggtgccggt
     4081 agctagtgat gtcagtcccg ggactggtga tgaggttgtt acccgggtgg tccccgtaag
     4141 taacgtggca gccagagatc tggcgccttt gctgcgtcag ctcaatgata atgctggcgc
     4201 aggaagcgtg gtgcattatg aaccttctaa tgttttgttg atgaccggac gtgctgcagt
     4261 gatgaaacgg ttgatggaga ttgttgaacg tgtggataag gtgggtaatc gcagcgttgc
     4321 cacggtcccg ctcacctacg cgtccgcaac agacgtagcc agacttgtta cggaactgac
     4381 taaagaaaca gataagacag ctatacctgc ttggatgacg gcgaaactgg ttgcagacga
     4441 gaggacaaac tcagtgctcg tcagcggaga gccaatctcc caacagcgta tcatctccat
     4501 aattaagcaa ctggatcgtc aggaggatgt tcagggtaat actaaggtga tttacctgaa
     4561 atatgcgaag gcgaaggatt tagtggaagt cctgacaggt atcagcagca gtattgaaaa
     4621 cgactctaaa aagagtccgt caacggaagc cttgcgcaaa ggagtgacga ttaaatccca
     4681 cgaacaaacc aatgccctga tcctgacggg ggcccctgac gtcatccgcg accttgaaaa
     4741 tgtgatttcg cagttggata ttcgtcgtcc tcaggtcctg gtggaggcca tcattgctga
     4801 aatacaggat gctgacgggc tgaaccttgg gatccagtgg gtgaataaac atgccggtgt
     4861 ggcgcagttc accagtaccg gtttacctat taccacgatg gttcagactc gtcagaacga
     4921 aatcttagac agcgatcaga gcaatgccct gagcatgttt aacggaattg cagcggggtt
     4981 ttatcaggga aactgggcga tgctgttgac ggcgctctcc acaagtagca agaatgatat
     5041 cttggcgacc cccagtattg tcacgctgga caatatggag gccactttca atgttggtca
     5101 ggaggtcccg gtactttcgg gctcacagac aacctctggg gacaatattt ttaacacggt
     5161 cgagcgcaaa acggtgggga tcaaactcag ggtaaaaccc cagatcaacg agggtgattc
     5221 cgtgttactg gagatagaac aggaggtgtc cggtgtggcg gacactgcag tagccaccac
     5281 tactgacttg ggagcaacct tcaacacccg aacagtgacc aatgccatgc tggtcgggaa
     5341 tggcgaaacg gtggtggtcg gaggattact ggataagtcg atcaggggga gtgagagtaa
     5401 agtgccactg ctgggggata tcccggtact ggggcatctt tttcgcgcaa aaagcgaaca
     5461 gacagctaag cgtaatctga tgctgttcat tcggccaact attattcgtg agcgcgacgg
     5521 atttcgtcat gcttcggccg aaaaatacca gtcgtttaat caggaacagg tgcagtcgcg
     5581 tggcaaagaa acaacggcgc tgacgctgaa tgaggaacag ctcaggctgt cccccgatca
     5641 agacgatacg gctttccgga aggtgaaagc ggcgattgct gcgttttatg cgcaggagat
     5701 gtaatgagca gagtagtaca gaatgtcagt gaatcacgtc cccttctgcc tttttccttt
     5761 tcccgtacgc agagaatttt gttgctgcgt gaacaggagg ggaatcgagt gttctgcatg
     5821 gaggacacac ctgccagtgc cctgttggag gtgcgcaggg tggctgaagg tcctctgaat
     5881 gtcacaacag tgtcggcgga agcgtttgaa aaacagctgg tgagcagcta tcaacgtgat
     5941 tctgatgaag cgcgacaaat gatggctgag attggtaatg aaatggattt ttataccgtc
     6001 gcaggggagt tgcctgaccg tgaggactta ctggatgcga atgacgatgc gccaattatc
     6061 cgcctgatta acgcaatgct gacagaggca attaaagaga aagcctcaga tattcatatt
     6121 gaaacttacg aacgccatct gcaggttcgc tttcgcattg atggcgttct gcgggagatc
     6181 ctccgtttac ataggaatct ggcttcgtta ctgatttcac gcattaaagt catggcccgg
     6241 ttggatattg ccgaaaaacg cgttccccaa gatggccgca tggtactgcg tatcggtggt
     6301 cgggcggtgg atgtgcgtgt ttcaacgttg ccttcaaatc atggtgaacg catcgtgctg
     6361 cgtttactag acaaaaatag cgttagtctc gatcttgctg cactcggcat gtcgcagcag
     6421 aatcagcgac acattgatgc actgatccgc cgtcctcacg gcattatttt ggtgaccggg
     6481 cctacagggt cgggtaaaag cacgacgctt tatgccgcgt taagcctgct gaatccccga
     6541 gaccgcaaca ttatgacggt cgaggatcct gttgaatatg aactggacgg tatcagtcag
     6601 acccaggtca acccgaaggt ggacatgacc tttgcccgga gtctgcgcgc tattttacgt
     6661 caggatccgg acgtggtgct tgtgggggag atccgtgacg gtgaaacagc ccagattgct
     6721 gtgcaggcct cgctgacagg gcatttggtg ctgtcaacgt tacatacgaa tagtgcagca
     6781 ggcgcgctgt cgcgtctgca ggatatgggc atccctcctt ttctgctttc cacctcatta
     6841 ctggctgttc tggcccagcg actggttcgc acgttgtgtc cgcgctgtcg tcagccctgt
     6901 caggtcagta ctgagttagc gatggacatg gacattcccc ctgaaaccac gatctggcag
     6961 cctgccgggt gtcagcactg cagtttcacg ggctatcacg ggcgcaccgg gatccacgag
     7021 ctgttgctga ttgacgatcg cattcggacg gcaatctatc agggggaggg ggagctgggt
     7081 attacccgct tggcagggag tcgctatctg acgctgcgtg gcgacgggcg acaaaaggta
     7141 ctagccggtg agaccagttg ggaggaggtg gttcgcgtta ctgaaagcag attgcaggaa
     7201 gaggaatgat atggcgttgt ttcattatca ggcatcagat attcacggta gaaaacgcag
     7261 tggcattctg gaggcggatt cggcccgaca tgcccgtcag ttgctgcgtg agcaggcgct
     7321 tattccagtc aggttggatg agaaacaggt ccatcacaag cactcactgc ggagtatcct
     7381 gaggtttcgt ccgcgcgggg ggagcagcgc cgaactcgcg ttactgacac ggcagttagc
     7441 cacactagtg gccgcatctc tgccgcttga agaggcgctg gatgcgttgt tgaggcagag
     7501 tgaaaaaccg cgtcagcgga acttaatcgc tgccgtgcgc accaaggttc tcgagggaca
     7561 ttccttggcc gcggcgatgg gcatgtttcc aggtacgttt gagcgtcttt attgtgcgat
     7621 ggtggcggcg ggggaaacat ccggtcgttt ggacgtggtg ctcagtcgtc tggctgacta
     7681 taccgagcag cgccagatca tgcgaaaccg tctactccag gcattgctct atccttgtgt
     7741 tctgacgttg gtggcggttg gggttattgc cattctgctt actgcggttg tgccgaaagt
     7801 ggttgagcag tttattcata tgaaacagac ccttccctta tctacccgcg tactgatggg
     7861 ggctgctgag gtgagccaga cttggggccc gtggctgtta ctggccgcag cgctgggcgg
     7921 gatagcgggg cgcatgatat tacatcagcc ctcccagcgt ctggcttttc atcatctgct
     7981 gttgcggctg ccggtcgtgg ggcgcatatc gcgcggtctg aataccgcac gttatgcccg
     8041 cacgctgagc attcttaatg ccagtgcggt accgctgctc caggcgatgc acatcagtgg
     8101 tgacgtactg agtaatgact gggcccgtca tcagttagct accgcggccg agttggttag
     8161 ggaaggggtc agcctgcatc aggcactgga gcagacttcg ctgtttccgc ctatgatgcg
     8221 gcacatgatt gccagcggtg aaaatagtgg cgaactcgac agcatgttgg aacgggccgc
     8281 cgacaatcag gatcgtgagt tcagcacaca gatgcaactg gcgctgggat tgtttgaacc
     8341 gctgttggtg gtgggtatgg ccggggtcgt tttgtttatt gttctggcaa ttctgcagcc
     8401 gctcctgcag ctcaacaaca tgatgaatat gtgaatcctt ctttaacagt caacggagaa
     8461 aaacatgcga aaacagcatc agcgtggttt taccttgctg gaaataatgg tggtaattgt
     8521 gatcttggga gttttggcca gtctggtggt cccgaatctg atgggaaata aggataaggc
     8581 cgatcggcag aaagtgatga gcgacttggt tgcgctggaa agcacgctcg atatgtaccg
     8641 gttagataat aaccggtatc cgacaacgga gcagggactg cgggcactgg tcagtaaacc
     8701 caccgtgcaa cctgaacccc gtaactaccg gcaggatggt tatatccgcc gtttgcccca
     8761 ggatccttgg gggggggatt atcagctgtt aaatcctggt cagtacagcg acattgatat
     8821 cttttctcct ggcccggatg gtgtgccaaa cactgaagat gatattggta actggacgct
     8881 cggtaacgcg cagccatgag tcagcgcggt ttcacaatac tggagatgat gctgatcctt
     8941 ttgttgatgg gcatcactgc agggctggta ctaatgtcat ttcctgactc tgctcagaat
     9001 catctgcaac aacagcggga gcgtctgcag gcgcagctgg attatgcctt ggatagaagc
     9061 cagcaggacg gcttgctgat ggggattcag gttcggtttg acggatggaa atttaaggtt
     9121 ctgcagcgcg gaacagcgga aagccccccg acattagctg agggagggga tatttggcag
     9181 gggtatgtct ggcaaacttg gcaaccgcga cgtgcagcca tggggggcaa acttccggat
     9241 gatgtgcgtc ttgagctgca gtatctcaga ggtttgcagt ggatgtctga gcatgatgac
     9301 ggggctgaac ccgatatttt gctgcttccc ggaggtgagg taacgccgtt cagacttctt
     9361 ttcaggcagg tcggagagga ggccgttgtc gggctgcagg ttgatgagaa cggtttgatg
     9421 actctttttg aaggtgaggt ctcattgtga aggggcaaga aggaatgaca ttactggaag
     9481 tcatagtagc tctggttgtg tttgcgctgg caggaatggc gctgatgcag gcctcgaccc
     9541 agcaggctgc ggggatcgga cggatggaag agaaggtgct ggcaggctgg ttggccgata
     9601 accagatggt gcaattgcaa ctcgagaaaa cttggccgga aaatggctgg ggcgagaaga
     9661 cgatcagctt cgctggcacc gaatggtatt tacgctgggg cgggccggac agcgacgtgc
     9721 cgcagccgcg ctccttagag gtagaggtac ggcgcactaa agaggagacg tccgccttgg
     9781 tttccttgcg tagcagtgtg gttcgtgaat gagtcagcaa cgggtaaaag gttttacgct
     9841 actggaaatg ctgttggcac tggctgtttt tgcagcgttg agcatcagcg catttcaggt
     9901 gctgcagggt ggaatacggg cgcacgagct gtcccgggat aaagtacagc gtctggctga
     9961 gctgcagaga gggatcagcc agatggagcg agatcttacg cagatgctcc caaggcacag
    10021 ccgggggaat gaaggcctgc tgctcgctgc acctcatctg ctaaaaagtg atgactgggg
    10081 gatttctttt acgcgaaaca gctggctgaa tcccgccggg atgctgccgc gcccggagtt
    10141 acagtgggta ggttatcggt tgcgacagca aaagttagaa cgcctgagct actttcatgt
    10201 cgatcaccct tccggtgttt ctccggatgt cagggtgatg ctggacggcg ttcacgcatt
    10261 tcgtctgcgt ttttttgtta acggtgattg gcaggcgcgc tgggacagta ccggcatcct
    10321 gccgcaggct gttgaagtga cgctggttat ggatgatttt gctgaactgc cccgtctgtt
    10381 tcttgtgagc aaggagaccg ctgaatgaaa ttgcgggaac agggcgtggc gcttctggtg
    10441 gtattgctga tcctgtcgtt aatggtaacg atagcggcgg tgattgcgga gcgcaacggc
    10501 cggacatttt tgcgtacagt agcgcagttg gatcagctgc aggcaaagtg ggatggctat
    10561 acggcagaga cgatagcaaa acaaatctta cagaggagca ggcaggagtc gccccggaaa
    10621 acgcatctgg cacaaaattg ggcgcagtca gaacgacagt ttgagaccag agggggagat
    10681 gtgcgggggc agatcgtgga tgcgcaggcc tgttttaatc ttaatgctat taattatgga
    10741 gtggtggatc tgaccagtat accttatgcc gccagaattt ttcagcagct attaatcaac
    10801 ttgcaggtag agcttttaca ggcccggcaa gtgacggcag ccctacgtga ctggatcgat
    10861 cgtgatgata agccggtaag gggcggtgct gaagacgagg tgtatatggg gatggaacct
    10921 ccctatctgg cagccaacca gccgatgcag gacgtcagtg agttacgcct tatccgggga
    10981 atagatgcca ggctttaccg caaacttttg ccgtatgttt gtgtactgcc tacgtcagat
    11041 ctgtctgtga atgtgaatac tctgctggat tcacaggcgc cgctgctggc ggcgctcttt
    11101 ctgacgaaac cggacagtct tccggtgacg gaattactgc agcggcgacc ccgtactggt
    11161 tgggagagcg tggcggcgtt tcttgacgcg ccaccgctta aggacattga caccagtgcg
    11221 gccatgccgg tattggccgt cagcagcaac tattttctgg ttcggttaca tgtgcgctcc
    11281 ggggaacacc tgttcagtca gcagacttta atgcagtgga gggaggagcg tttccggatc
    11341 attcagcgac agtatggatt aacgatgagg gaggttccct gaaaaaaaat aacaaacatg
    11401 tcgaggctgg tcggcttttt ctgcgactgc cggaggatga ctcggagcct ttattgtgga
    11461 ggctgattgc gccagaggag acaggagccg gcagtgtggc agtcggagaa acggatccgc
    11521 agttagtcgg tttgctgtcc cgttatcccg cctgggtgct ggttccgtca ggcagaattg
    11581 ccttccatca ggtcacgctg ccacaccgca cgtggcgtca gcgtttgcag gtgctgccct
    11641 ttcttctgga agagcagttg gcgacagaca ttgagcagct gcacttcgcc attcttcatc
    11701 agtcgggtga caggtgtgat gtggcggtgg tcgaaaaagc ggttatgcat cgctggttag
    11761 cgtactgtgg acaacttggg gttcgggagg tgaggttgct gcctgacgta ctgatgctgc
    11821 cgttggcaac tgaaggatgg agtgccgtaa agcttgccgg gcagtggctg tttcgccggg
    11881 atcgttacgc cgggatgacg gtagagttat cgtggctgac acatttcttg tctcttactg
    11941 cgccgccggt gattgagtcc tactccgtgc cgccagtacc gtgcccgggt ccgaagacca
    12001 ctgagtggcg aaatcagccc ggacgcgatc tgctgcagtt ggtggccgaa ggtgacgggt
    12061 atgatggcgc agatctgcgg cagggagaat ttgcccgcca gggaacatgg tatgcacgat
    12121 ttcgcccatg gaggtatgtc gcaggggctc tgctggcctg tgtttttctg gccgggagca
    12181 acgcggggct ggcacattat cggctctggc agcaggctca attctggcgg tatgagagcg
    12241 tacgtgtcta tcagcagctg tttccgagtg aaaaggcggt gaaggatccc cgccggcaga
    12301 tgctgagaca tctgcaacag tcgacgggta acaatatacc ggaactcggg agcgtgatgc
    12361 gccagcttca gcaactgctg tcagaaacct caacgatacg cttacaggca ctcgcctggg
    12421 acagcagcag ccagacactg aaaattgatt tgcaggcggc ttcttttcag gcgctggaac
    12481 actttcagca gatcgccgga gaaaaatacc tcatccagcc tggtgagata cgacagcagc
    12541 cgcagggggt agaaagtcgt ctgatcctga gagtgaacaa tgaacgagct taaaaaacgt
    12601 ttgcagtatg ttagcccccg tgagcgcagg ctgcttatcg gatgtgttgc cttactggtt
    12661 gttgtttttg tctattacgt cctctggcag ccctggctga tacgagagga taagtggcgg
    12721 actgtagtta cccgtgaaaa aaacacagtg gagtggatga aacaacaggt acccaacata
    12781 aagagacggg aggcaagtat gcctgaacag ggggagccgt taggtttatc tgcggccgtg
    12841 acacgtactt cagccgcata cggtgtatcg gtcacccggt tgcagcctca aggggagcgt
    12901 ctggccgtca ccttagcccc ggttgaattt aatgcgctga tgcagtggct tacgcaactg
    12961 gagcgtcggc accgtattcg taccatggta tttgatgtgg ccgcgcaggg caacccaccc
    13021 ggacaggtaa cggttaatcg gctggttttg tcccaagatg acgtaaacaa ggacgcttcg
    13081 cttagtcgct gatgtcgtaa tttacagatt aatgcttgat gtaaaaattg gacttataaa
    13141 ggaggatgtg tgagtagttt gccggaattt gcggttgatt atcccttgtt gtggctcatc
    13201 tgcaccggag ggctgggggg cgttaccggc agttttctta acgtcgtgat tcatcgtctg
    13261 ccgataatgt tggagaggcg gtggcggcgt gaagcgctga cgctattggc acattctgtg
    13321 cctgagtccg ggccggcctt taacctgatg ataccgcagt ctcattgtcc ctgctgtttt
    13381 catccgctcg gcatcaggga taacattccc ctgttaagct acctatttct gaaggggaaa
    13441 tcacgctgtt gtggcgagcg tattcccgtt tactacccgc tagtcgaaat aacgaactca
    13501 gtgttattca ttctggccgc gagcaggttt ccaccagggc tgacattagc ggctgcctgg
    13561 ctttttattt ccatgctgtt ggttctggcg gttattgact gccatacggc gctgctgccg
    13621 gatgtgctga cgttgccatt gctctggctt ggcctgttat tcagtttaca gagaggcgtt
    13681 gtcaccttag aggaggcggt agtgggggcg gtatctgggt atctctgtct gtggggactg
    13741 tactggcttt ttcgtttcgc taccgggagg gaagggttag gttacggtga ttttaagcta
    13801 gctgcggcat tgggagcatg gatgggttgg caggcattac cctcaatact tttgtttgcc
    13861 tcagtcagtg ggttaatcgt aacagtactg ttgcgtattg taacagctac ggactttacc
    13921 cgcccgctgc cttttgggcc gtggctcgct ttgagcggag tgtgccattt tttgttgatg
    13981 tagcgttcgg ttaccggggc tagtaataaa aacggctcag cggataaaag aactgttgta
    14041 aaccggtatc aggttaaacg atacctagta aaaaatgatg ggtaatattt tgaaaaaact
    14101 gaattgtatc gcgtcattac tagtcttagt gactatctct ggttgtcatc agtcaccctc
    14161 catccataag caggccactg ttcctccgag cgaacaactg gaacaaatgg cgtcgattgt
    14221 ctctgcgaca cggtatctga aaatgagatg taaccgtagt gatttaccag atgaacagtc
    14281 gatcctaaat gtcgctaacc ggatcgctat cgggaaaggg tggcagtcat taactcagga
    14341 ggatatacgt aaacacagcg atgatatcta tgtgcgtctg actagggaca gtacgcctga
    14401 gtatataaaa tgcagagaat tcaatcggcg gctggtccca tttatcggtg aattattggc
    14461 aaggggtcgt ggataatatg aaccgccccg gaaatcctgg agactaaact ccctgagaaa
    14521 gaggtaaaca gcatgaataa aaatgggtct tcccttgtag tggtggctga aggcatgatg
    14581 attgtagtgg cacactgaat ttggccacct gaacagaggt gatatgctca cctcagaaca
    14641 acacaggtgc tccaatgaaa aaaagaaatt tcagcgcaga gtttaaacgc gaatccgctc
    14701 aactggttgt tgaccagaac tacacggtgg cagatgccgc caaagctatg gatatcggcc
    14761 tttccacaat gacaagatgg gtcaaacaac tgcgtgatga gcgtcagggc aaaacaccaa
    14821 aagcctctcc gataacacca gaacaaatcg aaatacgtga gctgaggaaa aagctacaac
    14881 gcattgaaat ggagaatgaa atattaaaaa aggctacaac cgcgctcttg atgtcagact
    14941 ccctgaacag ttctcgataa tcgggaaact cagagcgcat tatcctgtgg tcacactctg
    15001 ccatgtgttc ggggttcatc gcagcagcta cagatactgg aaaaaccgtc ctgaaaaacc
    15061 agacggcaga cgggctgtat tacgcagtca ggtacttgag ttgcataaca tcagccatgg
    15121 ttctgccggg gcaagaagca tcgccacaat ggcaaccctg agaggcttca gaatggggcg
    15181 ctggcttgcc ggcaggctca tgaaagaact gggactggtc agttgccagc agcctgcgca
    15241 ccgttataaa cgaggtggtc gtgaacatgt cactatcccg aatcaccttg ggcggcagtt
    15301 cgcagtgaca gagccaaatc aggtatggtg cggcgacgtg acgtacatct ggacggggaa
    15361 acgttgggca taccttgccg ttgttctcga cctgtttgca aggaaaccgg taggttgggc
    15421 aatgtcgttc tctccggaca gcagactgac catcaaagcg ctgaaaatgg cctgggaaat
    15481 ccgcagtaaa ccagccgggg taatgttcca cagcgatcag ggcagccact atacaagcag
    15541 gcagttccgg cagttactgt ggcgttacca gatcaaacag agtctgagtc gacgaggaaa
    15601 ttgctgggat aacagcccga tggagcgctt cttcaggagt ctgaaaaacg agtggatacc
    15661 ggtgacgggt tacatgaact tcagcgatgc tgcccatgaa ataacggact atatcgttgg
    15721 gtattacaac gcgctcaggc cgcacgaata taacggtggg ttgccaccaa atgaatcgga
    15781 aaaccgatac tggaaaaact ctaaagcggt ggccagtttt tgttgaccac tacatcccgt
    15841 cagccgattt acgggccgta agtaacgaag ttggatgcaa atgtcagatc gtgtgcgcct
    15901 gttatggctc ggctggtaag agagccttat aggcgcattt gaaaaaactc cagctatacc
    15961 ggaagagatt tattattaac aggagaggtt tgcttttctg tgtaggttta gattttgtgt
    16021 ttgcagggta aacaaaactg attaactcgt catttcggta actgaaatag tttgctgtat
    16081 tctgtttaca atctctttaa taaggcattt gtcacgtggc tattcatatg aaaatcatac
    16141 gttatcttac aaatcaatca tctgagtgtt ataatataac ttagctgtga tatgtgtaag
    16201 aatgtttagg caatatatta ttatcgctaa tgttgttttg gttgggagag attctccttg
    16261 cggtagcttt ctgtttttag atgcttcttg cttaaaagga tataatttct gttcttttat
    16321 atagggttct ttacattta
//
LOCUS       DAZKIP010000099        16189 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZKIP010000099 DAZKIP010000000
VERSION     DAZKIP010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16189
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.100"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(488..913)
                     /gene="arsC"
                     /locus_tag="ACHOUK_003462"
     CDS             complement(488..913)
                     /gene="arsC"
                     /locus_tag="ACHOUK_003462"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFP9898841.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTTLG
                     TRLCRPSEVVLEILPDAQKGAFTKEDGEKVVDEAGKRLK"
     gene            complement(926..2215)
                     /gene="arsB"
                     /locus_tag="ACHOUK_003463"
     CDS             complement(926..2215)
                     /gene="arsB"
                     /locus_tag="ACHOUK_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFP9898842.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(2269..2622)
                     /gene="arsR"
                     /locus_tag="ACHOUK_003464"
     CDS             complement(2269..2622)
                     /gene="arsR"
                     /locus_tag="ACHOUK_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFP9898843.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPSWAAKIIEQAWRCEQEKVQVIVRN
                     LARQNCSVDSKNTCS"
     gene            3366..3449
                     /gene="dinQ"
                     /locus_tag="ACHOUK_003465"
     CDS             3366..3449
                     /gene="dinQ"
                     /locus_tag="ACHOUK_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFP9898844.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3503..4855)
                     /gene="gorA"
                     /locus_tag="ACHOUK_003466"
     CDS             complement(3503..4855)
                     /gene="gorA"
                     /locus_tag="ACHOUK_003466"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFP9898845.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNVEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(4927..5769)
                     /gene="rlmJ"
                     /locus_tag="ACHOUK_003467"
     CDS             complement(4927..5769)
                     /gene="rlmJ"
                     /locus_tag="ACHOUK_003467"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFP9898846.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEPYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKSDGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            5972..8014
                     /gene="prlC"
                     /locus_tag="ACHOUK_003468"
     CDS             5972..8014
                     /gene="prlC"
                     /locus_tag="ACHOUK_003468"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFP9898847.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQVLREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8022..8774
                     /gene="rsmJ"
                     /locus_tag="ACHOUK_003469"
     CDS             8022..8774
                     /gene="rsmJ"
                     /locus_tag="ACHOUK_003469"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFP9898848.1"
                     /translation="MKICLIDETGTGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8823..10292)
                     /gene="dtpB"
                     /locus_tag="ACHOUK_003470"
     CDS             complement(8823..10292)
                     /gene="dtpB"
                     /locus_tag="ACHOUK_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFP9898849.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGTFTLFYMSINIGSLIAL
                     SLAPVIADKFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            10458..10511
                     /locus_tag="ACHOUK_003471"
     CDS             10458..10511
                     /locus_tag="ACHOUK_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9898850.1"
                     /translation="MVLNIVLAGDFDCERRP"
     gene            10489..10542
                     /locus_tag="ACHOUK_003472"
     CDS             10489..10542
                     /locus_tag="ACHOUK_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9898851.1"
                     /translation="MIANADRRPDKAFTLIR"
     gene            complement(10609..11043)
                     /gene="uspA"
                     /locus_tag="ACHOUK_003473"
     CDS             complement(10609..11043)
                     /gene="uspA"
                     /locus_tag="ACHOUK_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFP9898852.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11434..11769
                     /gene="uspB"
                     /locus_tag="ACHOUK_003474"
     CDS             11434..11769
                     /gene="uspB"
                     /locus_tag="ACHOUK_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFP9898853.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(11840..13339)
                     /gene="pitA"
                     /locus_tag="ACHOUK_003475"
     CDS             complement(11840..13339)
                     /gene="pitA"
                     /locus_tag="ACHOUK_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFP9898854.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            13571..14773
                     /gene="yhiN"
                     /locus_tag="ACHOUK_003476"
     CDS             13571..14773
                     /gene="yhiN"
                     /locus_tag="ACHOUK_003476"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFP9898855.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTVRLRSEVLSVAKDDTGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSC"
     gene            complement(15091..16143)
                     /gene="yhiM"
                     /locus_tag="ACHOUK_003477"
     CDS             complement(15091..16143)
                     /gene="yhiM"
                     /locus_tag="ACHOUK_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HFP9898856.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT         4179 a         4077 c         4206 g         3727 t
ORIGIN      
        1 tccacccggt aatattcgtg tataaaaaag ggcattgata gtatcgtaaa ggaaaacgtt
       61 aacaactatc aaaaagcgct cattttaaaa agttatgatg ttctccgata gtgtgtgaaa
      121 tgatattttt gtgatgtttt agatgtgtaa taataaaatg ggttaaggta tcttataatt
      181 attgaataat ttgttgtgta aagcactgat aaatcataat ccagtacgtt tttaaactgc
      241 tgcactgtat cgctgatcac taagcagctc actgatacat catgctggac gcgataattg
      301 gaatctcacg cagggagtaa tgtgtaaata gcaaaatcgc ctgatgcgct acgcctgtca
      361 ggcctacaaa taacatacaa tttattgaat tttcatgact ttgtagatcg gataagacgc
      421 gttagcgtcg catccgacat aaataaagcg cacttttcta acaacctgtg ggggggatat
      481 agccgcttta tttcaggcgc ttacccgctt catcaaccac tttctcgcca tcttccttgg
      541 tgaatgcgcc tttttgcgca tctggcagaa tttccagcac cacttctgaa gggcggcaca
      601 ggcgagttcc cagcgtcgtc accacaatcg ggcgattaat cagaatcggg tgctgaagca
      661 taaagtcgat taaccgatcg tcagtaaatt tatcttccgc aaggcccagc tcctcatacg
      721 gttcgacgtt tttacgcagc agcgcgcgta cggaaatccc catatcggca atgagtttga
      781 ccagttcatc gcgcgttggc ggagtttcca gataatggat aatggtcggt tctgtgccgc
      841 tgttgcggat catctccagc gtattacgcg acgtgccgca ggccgggttg tgataaatgg
      901 taatgttgct catatcagta tctcattaca aagtgaaaga gagacgtagc gccagcgcag
      961 ccagcgtcac aaacagcaca ggcagggtca tgataatccc tgtacggaag taatatcccc
     1021 agctgatagt catattcttc tgcgaaagta cgtgcagcca gagcagcgtt gccaggctac
     1081 caatcggggt gattttcggc cccaaatcgc agccaatcac gttggcataa accatcgctt
     1141 ctttgataac gccagatgcc gtgctgccat caatggacaa cgcgccaacc agtaccgtcg
     1201 gcatattgtt cataatagaa gagaggaagg cggtgaggaa tccggtgccg agcgtcgcgg
     1261 cccacaggcc gttatccgcc agcacgttga gtacgccaga aagatattcc gttaatccgg
     1321 cattgcgcag gccataaacc accagataca tgccgagcga gaagatgaca atctgccagg
     1381 gcgcaccgcg cagcacttta cccgtattaa tcgcatgacc gcgtttagcg acgacaaata
     1441 atatcaacgc gcccactgcc gcaatggcgc tgaccggaat gccgagcggt tccaggacga
     1501 aaaatcccac cagcagaagc agtaaaacaa cccagccagt tttgaacgta gcaggatctt
     1561 taatcgcttc tgcgggagat ttcagcagcg ccatgtcgta gttctgcgga atatctttgc
     1621 ggaaatagag atgcaacatc accagcgtgg caacaatcgc ggcgatatcc accggcacca
     1681 tcaccgaggc atattcgcga aagccgaggc caaagaaatc tgcggaaacg atattcacca
     1741 ggttggagac aataagcggc aggctggcgg tatcggcaat gaatccggcc gccatcacaa
     1801 acgccagcgt agtgccttta ctgaacccta aagccagcag catggcgatg acaatcggtg
     1861 tcaaaataag cgccgcgcca tcattggcaa acagggcggc aacggcagca ccgagcagga
     1921 caatccaggt aaacagcaag cgaccgcgtc cattgcccca gcgtgaaacg tgcagtgccg
     1981 cccattcaaa aaagccggac tcatccagca gcaggctgat gataataacg gcaataaacg
     2041 cagccgtcgc gttccagacg atattccaca ccaccggaat atcgcccgga tgtactacgc
     2101 ccgtaactaa cgccagtact gcgccgagcg ttgcactcca gccgatgcct aaacctttcg
     2161 gctgccagat aaccaataca atggtcagga caaagatagc gcctgccagt aacataatgc
     2221 ctcccggaaa aacacatgtg caaatccata tgtgtttaga taaatttttt aactacaagt
     2281 gttcttactg tctacggaac aattttgtct ggccaggttg cggacaatca cctgaacctt
     2341 ttcctgttcg catcgccagg cctgctcaat aattttcgcg gcccatgatg gaatatgcgg
     2401 tgataagcgg taatgaaccc acttaccctg cttgcgatcc agcaacaaac cgctttcacg
     2461 aagaagcgcc agatggcggg agatcttggg ctgcgactgg tcgagagcag tgcagagatc
     2521 acagacgcat aactctccca gctcgctgag cagtaaaacg atgcccagac gggtttcatc
     2581 ggcaagaatt ttgaacagtt ggatgggtaa cagaaatgac atattgcgct cctgattgtt
     2641 gctggtagtg tctctcttcg aagcggataa gtcaaaaaca tatatggttt aacgaatgtg
     2701 tatgtgcaga ggaaggtaat aggtgtgaat taagcgttgg ctattcattt gaaaggaggt
     2761 ctgaatcagg acgtaaaaaa accgactttg cgtcggtttt ttactttcca gcccagagtt
     2821 ggtggctctg gctggagtga gaatgcccac atctaacggt tcaatactat tagccagtgg
     2881 ctaacattgc aagcgttagc gatttcttca gctggagaag ggacagtttg ctatacatgt
     2941 tggtgaaaga ggatttatat actttcatcg ggtcgctgcc gtaccaggct ttgtccgaat
     3001 ccggatgtaa aaaaaccgac ttcgcgtcgg tttttttact cttcagccct gagttggtgg
     3061 ctctggctgg agtgagaatg ccatcatcta acgcatcaat actaatacca tactgaaact
     3121 attgcaagga catgctggtt ttataacctg aatatactgt atgattatcc agttagctct
     3181 gaggcatttt cactctggca atgcgcataa acgctttcaa agtcctgttc agtggtacgg
     3241 gtggtgccgt taactgatgc tctggccgga gtgagaatac cattatctaa caatagggca
     3301 tgcgccgtga caggcgatgg atgagtaagc ggatgcattc tcactccatc gtatggagaa
     3361 cacgggtgat tgataaagca atcatcgttc ttggggcgtt aattgcgctg ctggaactga
     3421 tccgctttct gcttcagctt ctgaactgat agcggaaacg taattaaggg caaagagcac
     3481 accactcttt gccctttaac atttaacgca tggtcacgaa ctcttctgcc gccgttgggt
     3541 gaatggcgac ggtattatcg aagtcttttt tggttgcgcc catcttcagt gccaccgcga
     3601 agccctgcaa catttcgtcc ataccaaaac caatgccgtg aataccgaca atcttctctt
     3661 ctggcccaac gcacaccagc ttcatgcggc acggctggcg gtgagtggtg acggcggtat
     3721 acatcgcggt gaaagaggat ttatacactt tcacctgatc gtcgccgtac tgctcgcgcg
     3781 cctgcggttc cgttaaacca acagtaccaa tcggcggatg gctgaagacc acggtcggaa
     3841 tgttgctgta atccagatgc tcatccggct tgttattaaa caggcgttca gagagacggc
     3901 gacccgctgc aactgccacc ggtgtcagtt ctactgcacc cgtgttatcg cccaccgcgt
     3961 aaataccttc aacgttggtg ttttgatatt tatcgacgac gatatagcct ttttcgttgg
     4021 ttttaacgcc agcggcttcg aggttgatgt tgtcattggc aggctcgcgg ccaatcgccc
     4081 aaatcaggca atccaccgtt tcactgcgac catcttccag ctccagcgtc aggctaccat
     4141 cggcattttt cactaccgct ttcgggatgg cgttggtgtg caactgcggg ccttcggcgt
     4201 tcatcacttc gaccagcgtt tcagagatca tcgggtcgaa gctgcgcagc ggcgcatgtt
     4261 tacgcacaaa cagatgcgtt ttcgcgccga ggccgttaat cacgccagcc agctcaacgg
     4321 cgatgtaacc cgcgccaaca accgccacac gctctggcaa agcaggaagg gcaaagaaac
     4381 catcagaatc aataccgtat tccacgcccg gaatatccgg gtggctcgga cgaccgccgg
     4441 tggcgatcag aatatgatcg gctgtgatgg tttcaccgtt aacttccagc gttttggcat
     4501 caacgaagcg ggcaaagcct ttgattacat caacgttatt tttaccgagc acgttttcat
     4561 aggaagtatg aatacggtcg atataggcgg tacggctggc gatcaacgtt tcccagttga
     4621 atttattgat agtggtatca aaaccataat ccgggccgta catatggatc gcttcacgga
     4681 tttgcgccgc gtgccacatc actttcttcg gtacgcagcc gacgttcacg caggtgccgc
     4741 ccagctcttt ggcttcaatc agcgcgcatt tctgaccgta catagccgcg cggttgatgg
     4801 aggcgatacc gccgctgccg ccgccgatgg cgatgtaatc atagtgttta gtcatgacag
     4861 agtgtcctta tcgttgatta ccgcgattgt agcgcgagac gtaataggtg ccagcaatgg
     4921 ctgcaattac tctggcacga tccagcttac ggtggcgtgc ccggtgccag ctggaaccag
     4981 tttgctgtgc agccacggca gcacgttatt catctgctgt tccagtttcc acggcgggtt
     5041 aatcacaatc atgccggaag cggtcatgcc acggcgatcg ctgtctggca gtaccgccag
     5101 ttcaatttgc agaattttgc gaatgccggt cgcttccagg tcgtggatca tgcgcttaat
     5161 ttgctggcgc agcaccaccg gataccacaa tgcgtaagtg ccagtggcga aacgtttgta
     5221 accttctgct atcccgctga ctaccgcctg atagtcggtt ttcatttcat acggcggatc
     5281 gataaggatt aaaccacggc gagaaaccgg cggcagtttg gccttaagct gctggaaacc
     5341 gtcggatttt tcgacgcgcg cacggctgtc tttctgaaat tcagaacgca gcaacgggta
     5401 atcgctcggg tgcagttcgg tcagttgcag gctgtcctgt tcacgcagta actgacgggc
     5461 aatcaacggt gaaccggggt agtaacgcaa ctgaccgcta cggttgaagt gttttaccac
     5521 attgatgtac ggctccagct ccgcaggcaa atcgtcctgc tgccagatac gggcgatacc
     5581 ttcgagatat tcgccggtac gctcggcatg ttcgctgcct aactgataac gcccggcccc
     5641 tgcgtgggtg tcgagataga gaaacggttt atctttctct ttcagcgact cgatgatcag
     5701 gctctgaacg gtatgtttaa ggacgtcggc gtggttgcca gcgtgaaagc tgtggcgata
     5761 actgagcatg ggtaaaggtg ttccggtaag taaaaatcgg cccgtattcg gggcgcacaa
     5821 aagcctatca ggacagtata ccgaaaagag gccgccgccg cgaaagcgta acgtttctca
     5881 ttgaaattca ctacacttaa ccccatgcta caaacattat gtaaagcgcc tgttgagcgc
     5941 ttccttaacc tctttaacca ggactgcgct aatgacgaat ccgttactga ctccctttga
     6001 attgcctccg ttttctaaaa ttctcccgga acatgtcgtt ccagccgtga ctaaggcatt
     6061 gaatgactgc cgcgaaaatg tggagcgcgt agtagcgcaa ggagcaccgt acacctggga
     6121 aaatctctgc cagccgttgg cggaagtgga cgatgtgttg gggcgtattt tctccccggt
     6181 cagccacctg aactcggtga aaaatagccc ggaattgcgc gaagcgtacg aacaaactct
     6241 gccgctgctg tcggaataca gcacctgggt agggcaacat gaagggctgt ataaagcgta
     6301 ccgcgacctg cgcgatggcg atcattacgc cacgctgaac acggctcaga aaaaagcggt
     6361 tgataacgca ctgcgcgatt ttgaactctc aggcatcggt ctgccgaaag agaaacaaca
     6421 gcgttacggc gaaattgcca ctcgtctttc tgaactgggc aaccagtaca gcaacaacgt
     6481 ccttgatgcg acgatgggct ggactaaact cgttaccgac gaagcggaac tggcggggat
     6541 gccagaaagc gcgctggctg cggcaaaagc ccaggccgaa gcgaaagagc tggaaggcta
     6601 cctgttgacg ctggatatcc caagctactt gccggtaatg acctactgcg acaaccaggt
     6661 cttgcgtgaa gagatgtatc gtgcttacag cacccgcgct tcagatcaag gtccaaacgc
     6721 cggtaaatgg gataacagta aggtgatgga agagatcctc gcgctgcgtc acgaactggc
     6781 gcaactgctg ggctttgaaa actacgcgtt taaatcactg gctaccaaaa tggcggaaaa
     6841 cccgcagcag gtgcttgatt tcttaaccga tctggcaaaa cgcgcgcgtc cgcagggcga
     6901 aaaagagctg gcgcaactgc gtgccttcgc caaagccgaa tttggcgtgg atgagttgca
     6961 accgtgggat atcgcatact acagcgaaaa acaaaaacag cacctctaca gcatcagcga
     7021 cgaacaactg cgtccgtact tcccggaaaa caaagcggtt aacggcttgt ttgaagtggt
     7081 aaaacgtatt tacggcatca ccgctaaaga gcgtaaagat gttgatgtct ggcacccgga
     7141 tgtacgtttc ttcgaactgt atgacgagaa caacgaactg cgcggcagtt tctacctcga
     7201 cctgtatgcc cgtgaaaaca agcgcggcgg ggcgtggatg gatgactgcg taggccagat
     7261 gcgtaaagcc gatggttcgc tgcaaaaacc ggtcgcgtat ctgacctgta acttcaaccg
     7321 cccggtgaat ggtaaaccgg cgctgttcac tcacgacgaa gtgatcactc tgttccacga
     7381 gttcggtcac ggcctgcacc atatgctgac ccgcatcgaa accgccggtg tttccggtat
     7441 cagcggtgtg ccgtgggatg cggtcgaact gccgagtcag tttatggaaa actggtgctg
     7501 ggagccggag gcgctggcgt ttatctctgg tcactacgaa accggcgaac cgctgccgaa
     7561 agagttgctg gataaaatgc tggcggcgaa gaactaccag gcggcgctgt ttattctgcg
     7621 ccagctggag ttcggcctgt tcgatttccg cctccatgcc gagttccgcc cggatcaggg
     7681 agcgaaaatc ctcgaaactc tggcagaaat caagaaactg gttgccgtag taccgtctcc
     7741 atcctggggc cgtttcccgc acgctttcag ccatattttc gccggtggtt atgccgcagg
     7801 ttactacagc tacctgtggg ctgacgtact ggcggcagat gctttctcgc gctttgagga
     7861 agagggcatt ttcaaccgtg aaaccgggca gtcgttcctc gacaacattc tgagccgtgg
     7921 cggttcagaa gagccgatgg atctgttcaa acgcttccgt ggtcgtgaac cgcagctgga
     7981 tgcgatgctg gagcattacg gcattaaggg ctgatcattc agtgaaaatc tgcttaattg
     8041 atgaaacagg caccggagac ggtgccttat ctgttctggc ggcccgctgg gggctggagc
     8101 acgatgaaga caacctgatg gcgctggtgt taacgccgga acatctggaa ttgcgcaagc
     8161 gtgatgagcc aaaacttggc ggcatctttg ttgattttgt tggcggagcg atggcgcacc
     8221 gacgcaaatt cggcggtggt cgcggtgagg cggtggcgaa agcggtgggc attaaaggcg
     8281 attatttgcc ggatgtggtg gacgctaccg cagggctggg gcgcgatgcc tttgtgctgg
     8341 cttcggtcgg ctgccgcgtg cggatgctgg agcgtaatcc agtcgttgcc gcgctactcg
     8401 acgacggcct ggcgcgtgga tatgcggatg cggaaatcgg cggctggttg caggagcgtt
     8461 tgcagttaat tcacgcctcc agcctgacgg cgctgaccga tattaccccg cgtccgcagg
     8521 tggtctatct cgacccgatg ttcccgcata agcagaaaag cgcgctggtg aaaaaagaga
     8581 tgcgcgtctt tcagtcgctg gtgggaccgg atcttgatgc cgatggatta ctggagcctg
     8641 cacgcttgct ggcgaccaaa cgcgtggtgg taaagcgccc ggactacgcg ccaccgctag
     8701 cgaatgtcgc cacgccaaac gcggttgtga ctaaagggca tcgctttgat atctatgcag
     8761 gtacgccagt gtaaaagtaa agcagggtag cgataacgct accctgcttt ctgcaagaat
     8821 aattaatggc tttccggcgt cgcaatcatg cgtttcagcc acggcaccat caacagcatc
     8881 actactgcaa cgcccagcgt gaccagacca atcttaccaa acacgttggt atagacaggc
     8941 aacgtctcaa gcggatcggt aatgttgtct ggtactgcgg taaatgttgc cacatagccg
     9001 cccagcaaga acgcggcagc ctgtgtcagg aaccacatcc cgagaataaa gcccatcaaa
     9061 tgctgcggca ccagagcagc aatcatcgcc aggccaaggg cgctaataaa cagttcacct
     9121 aagctctgga ataagtacac cagcacgata aaccatggcg atgtcagccc ttgtgcatcc
     9181 gcaaaccaca ttcccgcagc tgccgccgtc aaaaagccca gtgagcacat aaacatgccg
     9241 agagtaaatt tcatcggcat cgagaggtct ttgcctttgt tacccagatg cgtgtaaatg
     9301 cctgccagta ttgggctggc gagtaccacc cagaacgggt taagcgcctg gaagctgacc
     9361 gggttgatgg aaaaaccgag aatttcatga tgcacgttgt tgatggcaaa gaagttcagc
     9421 gatgttggca tctgggcgta gagaatgtaa aacaccaccg cttcgagcat caggacaaag
     9481 gcgacaaaca ttttattgcg cccggtttta tccagcttga atgcctgacg aaagaagatg
     9541 atggtgacga cgatggagag aacaatcagc accagattgg cgacttctac gttgtgcatc
     9601 agccaggcgc agacgaagat catcaccacg ctgccaagta atacgtacaa cagtttgctg
     9661 aagctcattg gccggaagtc gggctcagaa ccaatgtctt tcaccattcc acgacaggcg
     9721 atgtaaacta gtaatgcgat aattaacccc gcaccgcaca ggttgtaggt gactgaatag
     9781 ccgaatttat cagcgatcac aggggccagc gataacgcta tcaacgagcc gatattgatc
     9841 gacatataga acagggtgaa tgtgccatca aggcgcggat ctttcggcgg atagcacttc
     9901 gaaagcaagc tggctgggtt agctttaaac aggccgttac cgacagcgat agtccccagg
     9961 gcgatgaaaa tcaggtcagg cttaagtagc gacatgccgg tcatgaagta gccaatcgcc
    10021 agcacaagtg ctccaagaac gatggtgcgt ttggtcccca gcaggtggtc gccgacatag
    10081 ccgccaatgg aaatgaggcc atagaccagt gcagcaaaag caccaaaagt gacaaaagcc
    10141 tgctcttgcg agaatccaag ctgtttaacg aagaaaaccg ccagtacgcc ctgcacgccg
    10201 tagtagccga atcgctccca taactcgaca aaaaagatca tgaaaaatgg gcgaggttgc
    10261 tgcagcatcc ccatgggtgt tgttgtattc atatttatta accttccaat accatcctga
    10321 aaagtatgaa cgcgtaaggg ataacgcgcg aaaaagttta catctgtcag agattcttaa
    10381 tattgatgcg gtgaatagat taatacacta ttacaacaga aaataaccag ctgatcacac
    10441 taatgatgga aaatatgatg gtgttaaata ttgtattggc tggggatttt gattgcgaac
    10501 gcagaccgta ggccagataa ggcgtttacg ctgatcaggt aacaaggcgc gacaaaaagc
    10561 aaaacgcccg catatgcggg cgttcaggaa cacgtcgtgg agggaagatt attcttcttc
    10621 gtcgcgcagc ggaacaatca gcatatcaac gtgaacggtg ttgatcagct gacgtgcgga
    10681 agacatcagt ttgctccaga agtcctggtg gtgaccacaa accactagat ccatatcgta
    10741 tttcttgatt gcatcgacca gaacctggcc caggtcgccg ctgccgctca gggtttcagt
    10801 gattgggtag cctgcattag tggaaagctc ggtcagcgca tgatgtgtct cttcagagat
    10861 gcgtttctgc atatcaccaa gattcacatc aataagcccg gtatataggt cagagtagtt
    10921 tacatctacg tggatcagag aaactttcgc attgtatggg cgagccatag agactgcttt
    10981 ctctaccaga actttgcttt ccggggagag atcgaccgcg atgagaatgt gtttataagc
    11041 catagtgtta ctccttccat aaagttgtcg atgactggcc agctagcgtt tcttgtgccg
    11101 ttacgtcacc cgcgtcctgc gaactacgtg cgcggggcta acccagccgt aaagattcag
    11161 tgttaagacc atccttacct tatagcgacc cggatgatcc gtcaatccgc cttgcttacc
    11221 cgttagataa acaaattttc gacatgaata tattgatagt ggttaacctt ctggaaaaaa
    11281 aacaacctga tctcctacac tatctataga gccgctcgga tgttggtcac cacaaaagcg
    11341 gtttctccag gacctggtgt taactgattg gcagcgtatc gggagcggta gccccggggt
    11401 ggaactccgc gggcaggtcg ccggggagga ggtatgataa gcaccgtcgc attattttgg
    11461 gctttatgtg tcgtttgcat tgttaacatg gcgcgctatt tctcatcact acgcgcgttg
    11521 ttagtggtac tgcgtaactg cgatccattg ctctatcaat atgttgatgg agggggcttt
    11581 tttacctcac atggccaacc caacaaacag gtgcgtctcg tttggtatat ctatgcccaa
    11641 cgttatcgcg atcatcacga tgatgagttt attcgccgct gtgagcgggt gcgtcggcag
    11701 tttattctga ccagcgcatt gtgtggtctg gtggtggtca gcctgattgc cttgatgatt
    11761 tggcattaag cacttcagca ataataacgc ataaaaaaag cgggccagta aactgacccg
    11821 ctcgttttgg tgcgtactat tacaggaact gcaaggagag ccagtaaagc ccgccggaaa
    11881 gcagtaccgc agccggaagg gtaaacaccc aggccatcag aatgctggtc acggttttac
    11941 gctgtaagcc gccaccatct accaccatcg tccccgcgac agaagaggag agtacgtgag
    12001 tagtggaaac cggcatcccg gtataactcg ccaggccgat agacactgcc gcagtcatct
    12061 gggcagacat cccctgagcg taggtcatgc ctttcttacc gattttctca ccgatagtcg
    12121 ttgccacacg acgccagcca atcatcgtac cgatacctaa cgccagcgcg accgccatga
    12181 tgatccacac cggtgcatac tcgatggtgc taagcatgtc ggacttcagt ttcttcaaca
    12241 ggcgctgatc gtcagcactc acgccaggca tcttcaccac tttgtcgata gtgtcagaaa
    12301 cgcacagcat aatgcggcgc atctggctac gttgatcaag cgacagcttg tcgtagcttt
    12361 ccacatcggt ggtcaacata cctttcaggc ggttgagcgc gttaatggta ttcgacggat
    12421 ggcagtggaa ctccgcaggt tgcgttgcgc cagcttccgg agccggtact aactgatcag
    12481 caccagtagc ttgtttcagc agcgcaggat gctgctcaaa gtaagcttcg acgttgttga
    12541 tggcatcacg ggtacgggtg atttcgtagc cagtggcatt catgttcacc acgaagcctg
    12601 ctggtgcgac accaatcaat accaacataa ccagaccaat gcctttctga ccatcgtttg
    12661 cgccgtgcga aaacgccacg ccgatagcgg aaaggatcag cgcgatacgc gtccagaacg
    12721 gcggcttttt cttgccgtct ttcttttcac gctccgctgg ggtcaggtgg atacgggcgc
    12781 gtttcttggt gccgctccag tagcgacgca gcaagaaaat cagaccgcca gcaaacacca
    12841 ggccgacaat aggggaaacg atcagagaac cgaaaatact taatactttc gggatattga
    12901 gtgcatccac cactgacgtc ccggtcatca acgcattggt taaaccaatc ccgatgatcg
    12961 cgccaatcag cgtatgagag ctggatgcag gcaaaccaaa gtaccaggta cccaggttcc
    13021 agataatcgc cgccagcaac atagagaaca ccatggcaag gccatgagac gatcccatat
    13081 taagcagcag atccgtcggc agcatatgca caatggcata ggcaacactc agaccaccca
    13141 gcaaaacacc caaaaagttg aacaccgctg ccataaccac ggcgagctga gaacgcatcg
    13201 cgcgggtata gataacggtt gccacggcgt tggctgtgtc atggaaacca ttgatggctt
    13261 cgtagaacag cacaaaagcc agtgcaagca ataataacag cccggtatgc aaatccaggc
    13321 cagcaaacaa atgtagcata ggacgttacg ccattttgag gacatgaacg cggcgcatta
    13381 tcagtgactt tcgcggcgcg ggcaaagtga aatatagact ttttttgatt tgcctcctgt
    13441 atggatttca ctcaaaaaat aattatctta tataattcag gcaaatactt ccttttagta
    13501 atattgatgc tggtgcgacc actgaggaat ctttacaatt cacgcccgtt ttttctaaga
    13561 ggagcgcaac gtggaaaggt ttgatgccat tattataggc gctggtgcgg cgggtatgtt
    13621 ctgttctgcg ctggcaggtc aggcaggacg ccgggttctg ctgatcgata atggtaaaaa
    13681 accagggcgc aaaatcctta tgtctggcgg tgggcgctgc aactttacca acctttatgt
    13741 cgaaccgggc gcttatctga gccagaatcc gcatttttgt aagtctgcac tcgcgcgttt
    13801 tacccagtgg gatttcattg atctggtcaa taaacacggc atcgcctggc acgagaaaac
    13861 gttagggcaa ctcttctgcg atgactccgc gcagcagatt gtcgacatgc tggtggatga
    13921 gtgcgagaag ggcaatgtga ccgtcagatt gcgtagcgaa gtgctgagtg tggcgaagga
    13981 tgatacaggc ttcacgcttg aactgaacgg catgactgtc ggttgtgaaa agctggtcat
    14041 cgcgaccggc gggctgtcaa tgccgggcct gggcgcgtcg ccgtttggtt ataagattgc
    14101 cgaacaattt ggcctcaacg tgctgccgac ccgcgcgggt ctggtgccat tcactctgca
    14161 taaaccgttg ctcgaagagt tacaggtgct ggcgggcgtg gcggtgcctt ccgtgattac
    14221 cgctgaaaac ggcaccgttt tccgtgagaa cttactcttc acccatcgcg gcttgtctgg
    14281 accggcggtg ttgcaaattt ccagctactg gcaaccgggg gaatttgtca gtatcaatct
    14341 gctaccggat gtggacctcg aaaccttcct gaatgagcag cgtaacgcac atccgaatca
    14401 gagcctgaaa aacacactgg cggttcatct accgaagcgg ttggttgaac gcttacagca
    14461 actcgggcaa atcccggatg tttcgctaaa acagctcaac gtgcgtgacc aacaggcact
    14521 gattagcaca ttgaccgact ggcgcgtaca acccaacggc actgaaggct atcgcactgc
    14581 cgaagtgacg ctcggcggcg tggacaccaa cgaactctct tcacggacga tggaagcgcg
    14641 caaagtgcct gggctgtact ttatcggcga agtgatggac gttaccggct ggctgggggg
    14701 ctataacttc cagtgggcgt ggtcgagtgc gtgggcttgt gcgcaggatt tgattgcagc
    14761 gaagtcgtgc tgagctattt ttcctaaaga tgaaaattat tagagtcgca ttaaaaatga
    14821 gctaattttg atagtggcta tcttgtgatt attttctaat gagcccgtga actgaacccc
    14881 ccatgcttaa tataaggtgg agggaaaggt gattgaaaac tcacccagtg ggctgatctt
    14941 tataaaatat gaacaggttg aaaaaaaaac agtaactttg ctgtttttta tacttaacca
    15001 ctatgcatta atgctgcgtt atttcattct acctaagaaa aaccagcgtt acgcaaatgg
    15061 ttaacgctgg ttttatccgg tacgttgcaa ttatttttta gcagaacccg cttctaatat
    15121 tgaaacgatt gagaacaacg taaagcatac cgctcccaaa ccgaccagaa cccgcgaagg
    15181 aataaaatat cccatatcgg tctgcgccat ttccgcaaga aacgatgcta ggaaaaggca
    15241 aaacaggcag gtgaagacgg gaatcatcgg tattctgttg gctaacgaac aggtacgtct
    15301 ccagaccagt gccagtagcc agacttttga gaatatgctg taacagatca ttcccaagca
    15361 aatgaggata ataccgggag ccagtcgggc gcttgcatca gaactgacta agacgtatat
    15421 gccctgcagc accgtgattg agccgagaaa aataacccaa taacaccaca gccagtgctc
    15481 tttcgttgaa aacgtattac gtgtttgatg gacgattgtg gcaacaaggc caattagaca
    15541 ggcacagatt gcggttaacc cgagcaatac gtgaccggcg acataatgcg gagttatgtc
    15601 ggcactacgt aacagcgtaa tagaccaaat gaaaccgagc agggtgagta acacgggaac
    15661 ggcaatgagg caattaccga ttaatgaaga ataagcctgt accggtgttc cgtcgctctt
    15721 gctccccgct gcgtttttag gaatgagcag aaagtgaccg gatgacgctg ccaccgtcga
    15781 tacacaggcg gcaatcatac caacgccgaa aataacatgg ccggcgacaa actcgtctgc
    15841 catcacatca ttacctgcta acagtgccca accccatatc atggtgataa ttgaccccgc
    15901 atagccaata atggggaaca atgtattgta aaacgtatta acgccgcgcg tgagctgcga
    15961 aatgataata aatgcggtag tgaataatgc caggcatatg gctgccagag aaatgagcac
    16021 atgacccgcg acgaaatact cgctttgccc tgaactttcc agcacaaagc cacctaacgc
    16081 gatacaaatc aagcccataa ttaaagggat taatttgaaa agccacccga tatatatgtt
    16141 cactttaaac tcctcaggta tccctgctaa tattgaaatg tgcattaat
//
LOCUS       DAZKIP010000100        15852 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZKIP010000100 DAZKIP010000000
VERSION     DAZKIP010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15852
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.101"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..1094)
                     /gene="espY3"
                     /locus_tag="ACHOUK_003478"
     CDS             complement(<1..1094)
                     /gene="espY3"
                     /locus_tag="ACHOUK_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector pentapeptide repeat protein EspY3"
                     /protein_id="HFP9898857.1"
                     /translation="MMKTITKQPILFTDVPVADLRNSMKQDLNQNLIERLWNKIRDFF
                     LDSDKQKAFKSIHKYINTLSVLNYNSALTPDPNFNIDATSDLDSYLKLDFDRLSPKQK
                     QTTLCCFWNKIASSLPEPYNSTIKHNIIFYKDGENLMIRGTISIVNEVVKTYSLPIEK
                     DDNGYYDFSGLYLAHSNISGKDPNKDPDIDFGIDMGNCNCSNVNFEHTYFYGVKFTNA
                     NCTNANFNNCRFKKCDLTNMNCTGAILDNAMIYGKEKEPEMQYPEADQIIQRITYQKS
                     DGNETKGMILTNCSCVKTTFNWADLSESDCQNVDFSEANLSNTILPDIVRMKGTKLYR
                     TDLFNPILKTEAESTEEKDISPLAKIILDY"
     gene            complement(1325..2299)
                     /gene="yajO"
                     /locus_tag="ACHOUK_003479"
     CDS             complement(1325..2299)
                     /gene="yajO"
                     /locus_tag="ACHOUK_003479"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFP9898858.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFSRREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDMVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(2354..4216)
                     /gene="dxs"
                     /locus_tag="ACHOUK_003480"
     CDS             complement(2354..4216)
                     /gene="dxs"
                     /locus_tag="ACHOUK_003480"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFP9898859.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDETLILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            complement(4241..5140)
                     /gene="ispA"
                     /locus_tag="ACHOUK_003481"
     CDS             complement(4241..5140)
                     /gene="ispA"
                     /locus_tag="ACHOUK_003481"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFP9898860.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKAQDLIDDARQSLKQ
                     LAEQSIDTSALEALADYIIQRNK"
     gene            complement(5140..5382)
                     /gene="xseB"
                     /locus_tag="ACHOUK_003482"
     CDS             complement(5140..5382)
                     /gene="xseB"
                     /locus_tag="ACHOUK_003482"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFP9898861.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            5588..7036
                     /gene="thiI"
                     /locus_tag="ACHOUK_003483"
     CDS             5588..7036
                     /gene="thiI"
                     /locus_tag="ACHOUK_003483"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFP9898862.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     DGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTG
                     QEVVEVETVNDFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFKNVKVYRP"
     gene            complement(7090..7680)
                     /gene="yajL"
                     /locus_tag="ACHOUK_003484"
     CDS             complement(7090..7680)
                     /gene="yajL"
                     /locus_tag="ACHOUK_003484"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFP9898863.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLIRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(7643..8554)
                     /gene="panE"
                     /locus_tag="ACHOUK_003485"
     CDS             complement(7643..8554)
                     /gene="panE"
                     /locus_tag="ACHOUK_003485"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFP9898864.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAACRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRTLRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            8722..9213
                     /gene="yajQ"
                     /locus_tag="ACHOUK_003486"
     CDS             8722..9213
                     /gene="yajQ"
                     /locus_tag="ACHOUK_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFP9898865.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            complement(9341..10705)
                     /locus_tag="ACHOUK_003487"
     CDS             complement(9341..10705)
                     /locus_tag="ACHOUK_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9898866.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLRNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFDVEQA
                     IRQA"
     gene            complement(10854..11744)
                     /gene="cyoE"
                     /locus_tag="ACHOUK_003488"
     CDS             complement(10854..11744)
                     /gene="cyoE"
                     /locus_tag="ACHOUK_003488"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFP9898867.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(11756..12085)
                     /locus_tag="ACHOUK_003489"
     CDS             complement(11756..12085)
                     /locus_tag="ACHOUK_003489"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFP9898868.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(12085..12699)
                     /locus_tag="ACHOUK_003490"
     CDS             complement(12085..12699)
                     /locus_tag="ACHOUK_003490"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFP9898869.1"
                     /translation="MATDTLTHATVHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(12689..14680)
                     /gene="cyoB"
                     /locus_tag="ACHOUK_003491"
     CDS             complement(12689..14680)
                     /gene="cyoB"
                     /locus_tag="ACHOUK_003491"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFP9898870.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(14702..15649)
                     /gene="cyoA"
                     /locus_tag="ACHOUK_003492"
     CDS             complement(14702..15649)
                     /gene="cyoA"
                     /locus_tag="ACHOUK_003492"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFP9898871.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGFATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNSMSDMAAFEKLAAPSEYNQVEYFSNVKPNLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
BASE COUNT         3972 a         4090 c         3990 g         3800 t
ORIGIN      
        1 atatagtcaa gaataatttt tgctaatggg ctaatgtctt tttcttctgt tgattctgct
       61 tctgttttta atatcggatt aaaaaggtcg gtacggtaaa gtttcgtgcc tttcatcctg
      121 acgatgtcag gaagaatagt gtttgaaaga ttagcctcgc taaagtccac attttggcaa
      181 tcactttctg ataaatctgc ccaattgaaa gtggttttca cacaggagca gttagttaga
      241 atcatccctt tcgtttcatt cccatctgat ttctgataag ttatcctttg tattatttga
      301 tctgcttctg gatattgcat ctcaggttct ttttctttac catatatcat cgcattatca
      361 agaattgcgc cagtgcaatt catatttgtc aggtcgcatt tcttaaatct acaattgtta
      421 aaattcgcat ttgtacaatt cgcatttgta aacttgacgc cataaaaata agtatgttcg
      481 aagttgacat ttgagcagtt acagtttccc atatctatac cgaaatctat atcgggatct
      541 ttattgggat ctttaccgga aatattgctg tgtgctagat atagaccgct gaaatcgtaa
      601 tatccattgt catccttttc tattgggagt gaataagtct ttactacctc gttaacaata
      661 gagatagttc cacgtatcat cagattttct ccgtctttat aaaaaataat gttatgtttg
      721 atggtactgt tgtatggctc tggcaatgag gatgcaattt tattccagaa acaacaaagc
      781 gtggtctgtt tttgtttggg cgagaggcga tcaaaatcca gctttaaata tgagtctaaa
      841 tctgacgttg catctatatt aaagttggga tctggagtta acgctgaatt ataatttaga
      901 actgacaatg tattgatgta tttatgaatc gatttaaatg ccttttgttt atcactgtcc
      961 agaaaaaaat cacgaatttt gttccacagc ctttcaataa gattttgatt taaatcttgc
     1021 ttcattgagt ttcttaaatc tgcaaccggt acatcagtaa acaggatcgg ttgtttggtg
     1081 atggttttca tcatgattgt aatcctctta cgttgactaa ataagacagt tttaggggat
     1141 attacctgga gaaactatca aaaatcgctc aaagggatga agacgatgaa tagctgcaaa
     1201 ctcattcacg aagaatttct cttgcagagg tggattatgg ttacagaagg tgatgggagg
     1261 tacgttgtcc ccatcatcgc agacgatggg gaattcagca gataaaatgg gtgaaaagcg
     1321 aaatttactt aaatcctacg accggatgcg gtttatacgg tgtttccagt tcagcaatct
     1381 gttccggctt caaagtgata tccaccgcgt tcagcagctc atcaagctgt tcttcccgcg
     1441 aagttccgat aatcggtgcg gcaatgcccg gtttactcaa caaccaggcc agcgcaactt
     1501 gtgctcgtgt tgcaccgagt tcttcactga cgcccgttaa ccgctctgcg atctgcgcgt
     1561 cattttcatc gctttcttta tagagatttt tccccacctc atcagacacc agtcgtgcgg
     1621 tagtttctcc ccacggacgc gtcagtcgcc cccgcgccag cggactccac ggaatgaccg
     1681 ccacgccttc ctgataacac agcggcagca tctcgcgctc ttcttcgcga taaatcagat
     1741 tgtagtgatc ctgcatactg acaaactgcg cccagccgtg ctgtttttgt agttccagtg
     1801 cctgagcaaa ctgcgaagcg tgcatggatg acgcgccgat ataacgcgct ttcccggctt
     1861 ttaccatgtc gttgagggct tccagcgtct cttcgatcgg cgtgttgtaa tcccagcgat
     1921 gaatttgcag gatatcgaca taatccatgc cgagacgtcg caggctgtcg tcgatagagc
     1981 gcaaaatttg tgcacgggat aatccttccg gtaaatcacc aacgcgatgg aacactttgg
     2041 tggcaacgac cacgtcttca cgacgggaga aatcccgcag tgcgcgaccg acgatctctt
     2101 cgctgctgcc atcggaatag ctattggcgg tatcaaagaa atttatgccg ccttccagtg
     2161 cgcgtttaat gatgggacgg ctgctctctt ccggcagtgt ccatgcgtga ttaccacgat
     2221 ctggctcgcc aaaggtcata cagccgaggc aaagtcggga aacgcgaagg tcggtttttc
     2281 ctaaggggtt gtattgcatg ctgccactcc tgccatgaat aatttcttaa gcatagcagg
     2341 agtggagtag ggattatgcc agccaggcct tgattttggc ttccataccg gcggcatcga
     2401 ggccgagttc ggcgcgcatt tcttcctgag ttccttgcgg aataaagaag tccggcaggc
     2461 caatgttcag cacgggtact ggtttacgat gggccatcag cacttcgttc actccgctgc
     2521 ctgcaccgcc cataatggcg ttttcttcta cggtaaccag tgcttcatga ctggcggcca
     2581 tttccagaat taacgtttca tcaagcggtt tcacaaaacg catatcgacc agcgtggcgt
     2641 tcagcgattc ggcgactttc gccgcttctg gcatcagcgt accaaagtta aggatcgcca
     2701 gtttctcgcc acgacgcttc acaatgcctt tgccaattgg tagtttttcc agcggcgtca
     2761 gttccacgcc gaccgcgttg ccacgcgggt agcgcaccgc tgacgggcca tcgttatagt
     2821 gatagccggt atagagcatc tggcgacatt cgttttcatc gctcggggtc ataatgacca
     2881 tttccggtat gcagcgcagg taagagagat caaaagcgcc ctggtgggtt tgaccgtcag
     2941 caccaacaat gcccgcgcgg tcgatggcga acaggaccgg gagtttttga attgccacgt
     3001 catgcagcac ctgatcatag gcgcgttgca ggaaggtgga gtaaattgcc acgatcggtt
     3061 tgtagccacc aatcgccaga ccggcggcaa aggtcaccgc gtgttgctcg gcaatcgcta
     3121 catcaaaata gcgatccggg aatttacgtg aaaactcgac catgccggaa ccttcacgca
     3181 tcgccggagt aatcgccatc agtttgttat ctttcgctgc cgtttcgcac aaccagtcgc
     3241 caaagatttt tgaatagctt ggtaaaccac cgctactttt cggcaaacaa ccgctggagg
     3301 gatcaaattt aggcacggcg tggaaggtga ttgggtcttt ttctgccggt tcataaccac
     3361 gacctttttt ggtcatgata tgcaggaact gcgggccttt caggtcgcgc atgttcttca
     3421 gcgtggtgat aagccccagc acatcgtgac cgtcaaccgg gccgatgtag ttaaagccca
     3481 gctcttcaaa caacgtgcca ggcaccacca tgcctttaat atgttcttcg gtacgtttga
     3541 gcagctcttt aattggcgga acgccagaga aaactttttt cccgccttcg cgcagtgaag
     3601 agtaaagctt accggaaagc agctgcgcca gatggttgtt aagcgcgcca acattttcgg
     3661 aaatcgacat ttcattgtcg ttgaggacca ccagcatatc aggacgaata tcgcccgcgt
     3721 gattcatcgc ttcaaacgcc atgcctgcgg taatcgcgcc gtcgccaatc acacagacgg
     3781 tgcggcgatt tttgccttct ttctcggcag caaccgcaat accaattccg gcactgatgg
     3841 aggttgatga atgcccgacg cttaatacgt catattcgct ttcgccgcgc cacgggaacg
     3901 ggtgtagacc gcctttctga cggatggtgc cgattttgtc gcggcgtccg gtcaaaattt
     3961 tatgcggata agcctgatgc cccacatccc aaatcaattg gtcaaacggg gtgttataga
     4021 catagtgcag cgccacggtc agttcgaccg tgcccagccc ggaggcgaag tgcccgctgg
     4081 aacggctcac gctgtcgagt aaatagcggc gcagttcgtc gcagagtttc ggtaaactct
     4141 cttttggcaa cagtcgtagc tcctgggtgg agtcgaccag tgccagggtc gggtatttgg
     4201 caatatcaaa actcatcagg ggcctattaa cacttattgt ttatttatta cgctggatga
     4261 tgtagtccgc tagcgcttcc agtgccgaag tatcgatcga ctgttcagcc agttgtttca
     4321 gcgactggcg ggcatcgtcg atcagatcct gggctttctt ccgggcttgc tcaagaccca
     4381 ggagtgcagg gtaggtactt ttgccaagct gctggtcggc accctgacgt tttcccaacg
     4441 ttgcagtatc tcccaccaca tccaggatgt catcctgaac ctggaaggca aggccgatgc
     4501 tctctgcgta cttgtcgagt actggtagag cacgacgtcc tttatctccg gcgcttaatg
     4561 caccaaggcg aacggcggcg cgaattaatg cgcctgtttt atgacgatga atacgctcaa
     4621 gcgcgtccag aggtacgtgt ttgccttccg cgtctaaatc caacgcctga ccaccacaca
     4681 ttccggcaat accgctggcg ctcgccagtt cagaaatcat cgaaattctg tcgcgatccg
     4741 acacttctgg catatcagca tcgcttaaaa tcgagaacgc cagcgtttgt aaagcgtcgc
     4801 cagcgagaat cgcgttcgct tcgccaaact tcacatggca ggtcggcaag ccgcgacgca
     4861 gatcgtcatc atccatcgcc ggtaaatcat catgaattaa tgagtaagcg tggatacact
     4921 ctacggcggc agcgggtgcg tccagcgtgt ttgtgctaac gccgaacata tgaccggtgg
     4981 cataaaccag gaaaggtcgc aggcgcttac cacctaataa tgcgccatac tgcatggttt
     5041 cgaccacggg agtgttctga aagggcagtg gggcgataaa acggctcagc gcctggttgg
     5101 cctgcttaac gcaggcttcg agttgctgcg gaaagtccat tactcattgt ccggtgtaaa
     5161 aggggttaga gaggcgtctt cgttgtcaga cagcagaatt tgtacgcgct gttcggcttg
     5221 ttgtaatttg gcctgcccct gacgtgccag ctgcacgccg cgttcgaact cgttcagcgc
     5281 ctcttccagc ggcaggtcgc cactttccag acgggttaca atctgttcca gctcgctcag
     5341 cgccttttca aagctggcgg gcgcctcatt tttcttcggc ataatgaatg tctgactctc
     5401 aatatttttc gccccgtcat ggtaacggac tcagggcaaa tagcaaataa cgcgcaatgg
     5461 taaggtgatg tgcgcagcaa agcgatgtta gtggtatact tccgcgcctg gatgcagccg
     5521 caggtgtggg ctgctgtatt tttccctata caagtcgctt aaggcttgcc aacgaaccat
     5581 tgccgccatg aagtttatca ttaaattgtt cccggaaatc accatcaaaa gccaatctgt
     5641 gcgcttgcgc tttataaaaa tccttaccgg gaacattcgt aacgttttaa agcactatga
     5701 tgagacgctc gctgtcgtcc gccactggga taacatcgaa gttcgcgcga aagatgaaaa
     5761 ccagcgtctg actattcgcg acgccctgac ccgtattcct ggtatccacc atattctcga
     5821 agtcgaagac gtgccgttta ccgacatgca cgatattttc gagaaagcgt tggttcagta
     5881 tcgcgatcag ctggatggca aaaccttctg cgtacgcgtg aagcgccgtg gcaaacatga
     5941 ttttagctcg attgatgtgg aacgttacgt cggcggcggt ttaaatcagc atattgaatc
     6001 cgcgcgcgtg aagctgacca atccggatgt gactgtccat ctggaagtgg aagacgatcg
     6061 tctcctgctg attaaaggcc gctacgaagg tattggcggt ttcccgatcg gcacccagga
     6121 agatgtgctg tcgctcattt ccggtggttt cgactccggt gtttccagtt atatgttgat
     6181 gcgtcgcggc tgccgcgtgc attactgctt ctttaacctc ggcggcgcag cgcatgaaat
     6241 tggcgtgcgt caggtggcac attatctgtg gaaccgcttt ggcagctccc accgcgtgcg
     6301 ttttgtcgct attaatttcg agccggtagt cggggaaatt ctcgagaaaa tcgacgacgg
     6361 tcagatgggc gttatcctca aacgtatgat ggtgcgtgcc gcgtctaaag tggctgaacg
     6421 ttacggcgta caggcgctgg tcaccggcga agcgctcggc caggtgtcca gtcagacgct
     6481 gaccaacctg cgcctgattg ataacgtctc tgatacgctg atcctgcgtc cgctgatctc
     6541 ttacgacaaa gagcacatca tcaacctggc tcgccagatt ggcaccgaag actttgcccg
     6601 tactatgccg gaatactgcg gtgtgatctc caaaagcccg acggtgaaag cggttaagtc
     6661 gaagattgaa gcggaagaag agaaattcga cttcagcatt ctcgataaag tggttgaaga
     6721 agcgaataac gttgatatcc gcgaaatcgc ccagcagacc gggcaggaag tggtggaagt
     6781 ggaaaccgtc aatgacttcg gcccgaacga cgtgattctc gatatccgtt ctgtcgatga
     6841 acaggaagat aagccactga aagtcgaagg gattgatgtg gtttcactgc cgttctacaa
     6901 gttgagcacc aaatttggcg atctcgacca gaacagaacc tggctactgt ggtgtgagcg
     6961 cggggtaatg agccgcctgc aggcgctcta tctacgcgaa cagggcttta agaatgtgaa
     7021 ggtgtatcgt ccgtaatttg tggtttttac gtcgcatctg gtcagatgcg acgtttgccg
     7081 catccgacac tactcgtaat aattataaat ccctgccgcc atcaccagtt gtgatgccac
     7141 ttcatgggct ttctcgcgcc caaccagcag gtcgataatt ttcagaccaa agtcgatagc
     7201 tgtacccggc ccctggctgg tcagcaattt tacccgtgca tcccagacga cgcgcttgtc
     7261 ctgccattgt tcagcgggaa ttttgtcttt cagcgtcggg aagccggtca tattgccaat
     7321 cggaaagata tcgtgcggca ccagcacggt ggctggcgcg gcgcaaatag ccgcgacgat
     7381 acgcccggaa cggtggaact gtttaacggt ttcaaccagc agggtactat cgcgaaaaca
     7441 ctccgcgcct ttaatgccac caggcagcac gatcacgtca tattcgccat cagccacttc
     7501 gaccagcggc gcatccgcca gcagcttcac gccgcgcgag caggtaatcg ccaggttgcc
     7561 atcgctggct acgctggcag tggtgacttt gataccgccg cgaatcaaga ggtcgatcgt
     7621 ggtgacggct tcagtctctt cactaccagg ggcgaggcaa accagtgccg atgcgctcat
     7681 attcactctc ctttcttttt accatttcaa acaggcgggt gttttccggt acggcaatcc
     7741 catgcgcgcg ggcgcggcgt aagagaaaac cattgatata gtcgatttcg gtgtggcgca
     7801 gcgtgcggat atcctgcaac atcgacgaga tattttccgc tgtggcatca atcacctgca
     7861 tcacgtaatc acgtaaatct tccgctgaag tatgatgccc ttcgcgttcg atcactgccg
     7921 cgacttcttc gcatatttgc ataatttctt gcggatgatg acgtaattca ccgttcgggc
     7981 aattccagat ggcagtcagt ggattaatca cacagttgac tgccagcttg cgccacagct
     8041 cggcgcgaat attgttatgc caggcaacgt caggcaacac ggtttgcaat atatccgcca
     8101 gataactgta atcgccgtcc tgttgccgtg ctggaccaat atgcgtgata ccgtttgcca
     8161 catgaataat gacattgccg tcgcggcagg cggcatgggt ggtggtgccc atcagtaatg
     8221 gctgctgaat gttttgcaat tcttcgatgg tgcccatgcc gttgtgaatt aacagtattg
     8281 gcgtagttac aggcagtgtg gacgcgaggc ttttgacggc atcggaaacc tgccatgctt
     8341 tcagcgtcac caggagcaga tcgctggtgg caagaaaatc gggatcgttg gcggtcagcg
     8401 attcgttaaa tatcgaacca tcagtctcaa ccagattcac actacaataa ggttgcggta
     8461 cgcgcagcca gccctgaact tcgtgaccct gtttacaaag tgctgtaagc cataattgcc
     8521 ctaaggcacc gcatcccaat acggtaattt tcattgttcc tcctcacccg caaccactcc
     8581 gggtgttcaa taaggctatc ccttaattgt gcatgctgtt gcgactatgc acaattaagg
     8641 gatacgtcct ggtgcaggac tgttggttat ttaactttgc aggtattatg cttcgcatca
     8701 aaaatgaagg gagaggaaaa gatgccatct ttcgatattg tctctgaagt tgatcttcag
     8761 gaagcacgta acgcggtcga taacgcgagc cgcgaagtgg agtcccgttt tgacttccgt
     8821 aacgttgaag cctcatttga gctgaacgac gccagcaaaa ccatcaaagt gttgagcgag
     8881 tccgacttcc aggtcaatca gttgctggat attctgcgtg ccaagctgct gaagcgcggc
     8941 attgaaggca gttcactgga tgtaccggaa aatatcgttc atagcggtaa aacctggttt
     9001 gtggaagcga aactgaaaca gggcattgag agcgccactc agaaaaaaat cgtcaagatg
     9061 atcaaagaca gcaaactgaa agtgcaggcg caaattcagg gcgatgagat ccgtgtgacg
     9121 ggcaaatctc gtgatgattt acaggctgtc atggcgatgg tgcgcggtgg cgatctcggt
     9181 cagccgttcc agttcaaaaa cttccgcgat taatcgcgac aacctgcgct ttgttcatgt
     9241 cagatgcggc atgaacgcct ggttcggcct acaaagtttt gtaaatcagg aacttagtag
     9301 gcctgataag cgtagcgcag caggcaattt cgcctcgctt ttatgcctgg cgaattgcct
     9361 gttcaacatc aaagcgattc gtcactttgc tgtcgatttt cacataagct gaatgttctt
     9421 cttctgcaat caacacttct ttgatgcctt cagtttccag caaacgcact tttaacgcct
     9481 cgtttgcggc aatgttcgcc gggatttcaa tgcgcaaact gctgacatac ggcggttctt
     9541 tcatggtact ggcgactgcc agccacactg cggccagcat tgcgccagcg agaaataccc
     9601 cctgaccgtc aaacatgccg tcaatccagc cgcccagcga accgccaatc gccacgccaa
     9661 gaaactggct ggtggagtaa acacccatcg ccgtaccttt gtaacctgct ggcgactctt
     9721 tactgataag tgagggcagg agggcttcca tcaaattaaa cgccacaaag aaaagctgca
     9781 cgccgaccac cagttgccag aactgcgttt gcgcgttcct caacacaatt tccgcaacca
     9841 cgatcaaccc gacgcagaag acaaagactt gcttcatttt gcgcttaact tcagcgtaga
     9901 taatgaaagg cacgaccgag ccaaaggcga ttagcattgt cgccagatag accttccagt
     9961 gttcagccgc cgggaacccc gcatcagcca gttgtccggg cagggcaaca aacgtcgaca
    10021 tcagcaaaat atgcagacac ataatgccaa agttgagttt cagcagccgc ggttccgcca
    10081 gcaccttact gaaactgcct ttcaccattc cggactcacg attaagtacg tgagtgctac
    10141 tgttgggcac aacccaaatg gtcaacgcaa tgccggtcgt tgccagaata gcgatcatcc
    10201 agaacagcgc gtgcagccca agtttgtgag tgatgatcgg gccaagcacc atcgcaatgg
    10261 caaaggtaat gccaaagctc acgccgataa acgccattgc tttggtgcgg ttttgttcgc
    10321 gcgtgagatc ggaaagcagc gccataacgg cagcggcaat cgcaccggag ccttgtagcg
    10381 cccgacccag aataattccc cagatggagt cagagagcgc agcgataacg ctaccggcgg
    10441 caaacactgc cagcccaccg acaattaatg gtttgcgacc aatgcggtcg gaaagcaggc
    10501 caaacggaat ctgaaaaacg gcctgagtca gaccataaat accaatggca ataccgatta
    10561 atgcttcgct ggcaccttgc agagccatgc cgtacgtggt cagaaccggc agaaccatga
    10621 acatgcccag catgcgcaac gagaatacgg tccctaaacc ccaggtcgcg cgccgctcac
    10681 ctggcgtcat tttataatcg ttcattacca cctctgtttt aaattcgcga ctagtgtaaa
    10741 gcggcaaacg aatggggtaa acatgcggat tttttagtaa atatttcgag agggatatgt
    10801 ttctaatgct aagaaaaaag gtgccgtagc acctttttaa tagagaggtt gtgttaccac
    10861 acagcagcca gcagcgtatg cgagtccggc accataaaat caacggacat catcaccgag
    10921 agggcagtga tggcgatgat agagaagccg aacagcttgc gcgcccagat tctgtcatca
    10981 gcaactttat aaccgcgcag agccatacct aaccaccaga cgctaaccgc cgcggcgacc
    11041 accagatatt tatatccagc gtaaccgcca agagagagca tcagcgtggc aacggcaaag
    11101 gcgatgatat acagcgtgat gtgattcttc gccaccgaaa tgccttttac cactggcaat
    11161 accggaatgt ttgccgcctg gtaatcctta aagcggaaaa tggcgatggc ataggagtga
    11221 ggcatctgcc acaggctgaa gatagccagc aggatcgctg cgccgctatc gaactcgccg
    11281 gttactgcac agtagccaat caccggcggc gcagcgccgg agagcgaacc aatcaatgtg
    11341 ccgtagacgg agtggcgttt catgtacagg ctataaacgc cgacataaac cacaaagccc
    11401 atcaccccca gccagcaggc cagcggattc gcgccaaacc acagcagcat aaagccagca
    11461 atacccagca aggtggcgta caccagcgag acagcaggag agatcaggcc tttaaccagc
    11521 acccgattct tcgtcctttc catctttctg tcgatatccc tgtcgatgta gttgttaaac
    11581 acacaacccg acgccacaac cagtgacacc ccaaccagcg tgtagataaa caggggataa
    11641 tcaatgctgc cctttgaggc cagcaggaat cccccaatca ccgagatcag gttgccaaag
    11701 atgatgcctg gtttcgttac ttgcaggtat tgcttaaaca tcataaccgc cgctcttagt
    11761 gcatcatcat gttgtagttg aggttccaca taatccagat ggagcctaca actaggatag
    11821 cgatgattag cacggtgaag acaaacgccg tcatgttcca gccttcatct gatttggtat
    11881 tcatgtgcag gaagcacacc agatgcacca gaacctgtac cactgccatt gccaggattg
    11941 ttcccagaat tacggccgga gaggcagccc ctgtcatcac catccagaac ggaatcaccg
    12001 tcagaatgat cgacaggata aagcctgtca tgtaggtttt tacgctgcca tgggacgcgc
    12061 cgctgtgatc ggtagaatga ctcattacat cgcccccatc agataaacaa cagtgaacac
    12121 acagatccaa accacatcca ggaagtgcca gaacaggctc aggcacatga tgcgggtacg
    12181 gttagtgctg gtcaggccgc gacgagcgat ttgtaccatc agcaccgcca tccagataag
    12241 accggaagtg acgtgcagac cgtgcgtgcc gaccagcgca aagaacgctg acaggaagcc
    12301 gctgcgatcc ggacccatgc cgttaacaat caggtgatgg aattcgtaga tttccatccc
    12361 gataaatccg gcgccaaaca accaggtcaa cgccagccag gagataacct ggcttttgtt
    12421 gtttttgtac atggcgatag ccgccatgcc gtaggtgatg gagctgaaca acagcaagaa
    12481 agtttcaacc agaacgaacg gcagttcgaa aatgtcctta cctgtcgggc cgcctgcggt
    12541 gccgttcacc agaacggcat aggtagcaaa caagatagag aacagaatgc agtcgctcat
    12601 caggtagatc caaaatccga agattttggt tccgcctgca tcgtggtgcc cgtgttcgtg
    12661 cgcgtggaca gtcgcgtgcg tcaaagtatc agttgccatt tttcagccct gccttagtaa
    12721 tctcatcgaa atgctggttt tccagttttt cgatttctgc caccggcacg tagtaatcca
    12781 cgtcctcgtc gaagcttttc acgatccagg tgatgatcat gcctgcgaag ccaacaatcg
    12841 ccagccacca gatatgccag atcatggcga aaccgaagat ggtggagaaa gccgcaatga
    12901 cgatacctgc accgctgttt ttcggcatat gaatttcttc atagtggtca ggctttttat
    12961 acgcttcgcc tttctctttc atttcccaga atgcatcacg ctcgtgaacg tgcggcacaa
    13021 cggcaaagtt atagaacgga ggcggggaag aggttgccca ctccagcgta cggccacccc
    13081 acgggtcgcc agtcaggtca cggttctggt cgcggtcacg aatagaaacg tacatctgaa
    13141 taacgaggca gagaatacct agcgcaatca gtactgcacc gctggctgca atcatcagca
    13201 tggtgtggaa ctgcgggtca atctgctggc tcaaacgacg ggtcatgccc atgaagccca
    13261 gcgcgtacag tggcataaag gcaacgaaga agccgatgat ccagaaccaa aacgcgcgtt
    13321 taccccaggt ttcgttcagt ttgaaaccga acgctttagg ccaccagtag gtcatccctg
    13381 cgaagcagcc gaagaccacg ccgccgatga tcacgttatg gaagtgcgca atcaggaaca
    13441 ggctgttatg tagaacgaag tccgcgcccg gtactgccag cagcacgcca gtcataccgc
    13501 ccaccgagaa ggtgacgata aaaccgatag tccacagcat cgcagaatgg aacacgatgc
    13561 ggccctgata catggtgaac agccagttga agatcttcac cccggtcggg atggcgataa
    13621 tcattgtggt gataccaaag aaggcgttta cgttcgcgcc cgcacccatc gtaaagaagt
    13681 ggtgcagcca aacgatgaac gacagcacgg tgatacagac ggttgcccat accagcgagg
    13741 tataaccaaa cagacgttta cgcgagaagg ttgccgcaat ttcggagaac acaccgaaaa
    13801 caggcaggat caggatgtaa acttccgggt ggccccaggc ccaaatcagg ttgatgtaca
    13861 tcatcatgtt gccacccata tcgttggtaa agaaatgggt gcccagatag cgatccaggg
    13921 tcaacaacgc gacggtaacc gtcagaattg ggaaggaagc aataatcagt acgttcgcgc
    13981 acagtgatgc ccaggtaaat actggcatct tgaacatggt catgcccggt gcgcgcatct
    14041 tcagaatggt aacgaagaag ttgataccgg taagcgtcgt acctataccg gatagctgga
    14101 gactccatat ccagtaatcg acaccgactc ccggactgta ctctattccc gatagcggtg
    14161 gataggccag ccagccggtc tgcgcaaatt cgcccacgcc gagagaaacg ttaaccagaa
    14221 tcacaccaac aacggtaaac cagaagctta agttgttgag gaacgggaac gcaacgtcac
    14281 gcgcgccgat ctgcagcgga accaccaagt tcatcagacc gataacgaaa ggcatcgcca
    14341 cgaagaagat cataatcacg ccgtgcgcgg tgaagatctg atcgtagtgg tgaggtggca
    14401 ggaagcccgc ttcgcccgcc gaggcaagag cctgctggct acgcatcata atggcgtcag
    14461 caaaaccacg cagcaacatc acaatcgcca cgatgatata catgataccg aggcgtttat
    14521 ggtcgacgga ggtcagccac tctttccaca ggtaggtcca cttaccgaag taagtgatca
    14581 ggccaacgag cgccagacct cccaaaataa tgccagcgat cgtaaccatg acgataggtt
    14641 catggaacgg gactgcatca agtgataatt ttccgaacat ctttattctt cctcaacccc
    14701 tttaatgggc ggattccgcg tggctcatgt ccatgccttc cataccttcg tgtgcgctgt
    14761 gctcaccttc tggctgggtc atgtccatgc tcttaccgtg agccataaac ttgttaatta
    14821 catcggcaaa caagtttggt ttcacgttgg agaaatattc cacctggttg tattcgctag
    14881 gcgcggccag tttttcgaac gctgccatgt cagacatgga gttcggcgac tgtttcgctt
    14941 ttgcgaccca ctggtcgaat gcggcgcgat ccggtgttgc aatagctttg aacttcatgc
    15001 ctgagaagcc cgggccgctg tagctggcgg agataccgtc ataagtgccg ggttcgttgg
    15061 cgatcagatg caggcgagtc tgcataccgg ccatggcata aatctggcta cccagacgcg
    15121 gaatgaagaa ggagttcatc acggagttgg aggtcacttt gaagtacacc ggagtgttcg
    15181 ccgggaaagc gatttcattc acggtagcaa agccctgttc cgggtagatg aagaaccatt
    15241 tccagtccat ggaaaccact tcgatggtaa tgggcttctc gtcgtgtgcc agcggcttgc
    15301 taggctcaag agcgtgagtg gttttccagg tcagtaccgc aaggaagatg atgattaaga
    15361 taggtaccgt ccagaccaca gcttccactt tattggagtg tgaccagttc gggctgtact
    15421 tagcatcttt attgctcgca cggtacttcc aggcgaaacc aacagccatc aagattgcgg
    15481 gaataacgac aatcaacatc aggccaaatg ccgtcagtat cagtgaacgt tgctccagac
    15541 caatctgtcc tttgggatct aacagcgcag aattacagcc actgagcaat acagtgcctg
    15601 caaataatga caaccatccc aaacttttat tgtatttcct gagtctcatt taacgacctc
    15661 aattccacgg gatctggtgg cgtttaaaat gtgtgggcat tttacgggaa ggttacatta
    15721 ctgtaaacat gattaaattc ctgttacttg gatttggctg tcaggtcaca tatgttgcaa
    15781 aacatatcag gttgatttta agaagctgtt gcaaaagggc gttataacaa aaggggagtg
    15841 cagaagaagt ct
//
LOCUS       DAZKIP010000101        15458 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZKIP010000101 DAZKIP010000000
VERSION     DAZKIP010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.102"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            125..328
                     /locus_tag="ACHOUK_003493"
     CDS             125..328
                     /locus_tag="ACHOUK_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFP9898872.1"
                     /translation="MSSIFLTENELQTLTGCKYASHQRNWLIKNGLPFYTNRSGKPIV
                     SRELFTCIKTLPPREAEPDFGAI"
     gene            328..1375
                     /locus_tag="ACHOUK_003494"
                     /pseudo
     CDS             328..1375
                     /locus_tag="ACHOUK_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1403..1492)
                     /locus_tag="ACHOUK_003495"
     tRNA            complement(1403..1492)
                     /locus_tag="ACHOUK_003495"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1456..1458),aa:Ser,seq:cga)
     gene            1586..2383
                     /gene="mtfA"
                     /locus_tag="ACHOUK_003496"
     CDS             1586..2383
                     /gene="mtfA"
                     /locus_tag="ACHOUK_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFP9898873.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            2484..2559
                     /locus_tag="ACHOUK_003497"
     tRNA            2484..2559
                     /locus_tag="ACHOUK_003497"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2517..2519,aa:Asn,seq:gtt)
     gene            2873..10855
                     /locus_tag="ACHOUK_003498"
     CDS             2873..10855
                     /locus_tag="ACHOUK_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFP9898874.1"
                     /translation="MATKKRSGEEINDRQILCGMGIKLRRLTAGICLITQLVFPMAAA
                     AQGVVNAATQQPVPAQIAIANANTVPYTLGALESAQSVAERFGISVAELRKLNQFRTF
                     ARGFDNVRQGDELDVPAQVSENNLTPPPGNSSGNLEQQIASTSQQIGSLLAEDMNSEQ
                     AANMARGWASSQASGAMTDWLSRFGTARITLGVDEDFSLKNSQFDFLHPWYETPDNLF
                     FSQHTLHRTDERTQINNGLGWRHFTPTWMSGINFFFDHDLSRYHSRAGIGAEYWRDYL
                     KLSSNGYLRLTNWRSAPELDNDYEARPANGWDVRAEGWLPAWPHLGGKLVYEQYYGDE
                     VALFDKDDRQSNPHAITAGLNYTPFPLMTFSAEQRQGKQGENDTRFAVDFTWQPGSAM
                     QKQLDPNEVDARRSLAGSRFDLVDRNNNIVLEYRKKELVRLTLTDPVTGKSGEVKSLV
                     SSLQTKYALKGYNVEATALEAAGGKVVTTGKDILVTLPAYRFTSTPETDNTWPIEVTA
                     EDVKGNFSNREQSMVVVQAPTLSQKDSSVSLSSQTLSADSHSTATLTFIAHDAAGNPV
                     IGLVLSTRHEGVQDITLSDWKDNGDGSYTQILTTGAMSGTLTLMPQLNGVDAAKAPAV
                     VNIISVSSSRTHSSIKIDKDRYLSGNPIEVTVELRDENDKPVKEQKQQLNTAVSIDNV
                     KPGVTTDWKETADGVYKATYTAYTKGSGLTAKLLMQNWNEDLHTAGFIIDANPQSAKI
                     ATLSASNNGVLANENAANTVSVNVADEGSNPINDHTVTFAVLSGSATSFNNQNTAKTD
                     VNGLATFDLKSSKQEDNTVEVTLENGVKQTLIVSFVGDSSTAQVDLQKSKNEVVADGN
                     DSATMTATVRDAKGNLLNDVKVTFNVNSAAAKLSQTEVNSHDGIATATLTSLKNGDYT
                     VTASVSSGSQANQQVIFIGDQSTAALTLSVPSGDITVTNTAPLHMTATLQDKNGNPLK
                     DKEITFSVPNDVASRFSISNSGKGMTDSNGTAIASLTGTLAGTHMITARLANSNVSDT
                     QPMTFVADKDRAVVVLQTSKAEIIGNGVDETTLTATVKDPFDNVVKNLSVVFRTSPAD
                     TQLSLNARNTNENGIAEVTLKGTVLGVHTAEAILLNGNRDTKIVNIAPDASNAQVTLN
                     IPAQQVVTNNSDSVQLTATVKDPSNHPVAGITVNFTMPQDVAANFTLENNGIAITQAN
                     GEAHVTLKGKKAGTHTVTATLGNNNASDAQPVTFVADKDSAVVVLQTSKAEIIGNGVD
                     ETTLTATVKDPFDNAVKDLQVTFSTNPADTQLSQSKSNTNDSGVAEVTFKGTVLGVHT
                     AEATLPNGNNDTKIVNIAPDASNAQVTLNIPAQQVVTNNSDSVQLTATVKDPSNHPVA
                     GITVNFTMPQDVAANFTLENNGIAITQANGEAHVTLKGKKAGTHTVTATLSNNNTSDS
                     QPVTFVADKTSALVVLQISKNEITGNGVDSATLTATVKDQFDNEVNNLPVTFSTASSG
                     LTLTPGESNTNESGIAQATLAGVAFGEQTVTASLANNGASDNKTVHFIGDTAAAKIIE
                     LTPVPDSIIAGTPQNSSGSVITATVVDNNGFPVKGVTVNFTSNAATAEMTNGGQAVTN
                     EQGKATVTYTNTRSSIESGARPDTVEASLENGSSTLSTSINVNADASTAHLTLLQALF
                     DTVSAGDTTNLYIEVKDNYGNGVPQQEVTLSVSPSEGVTPSNNAIYTTNHDGNFYASF
                     TATKAGVYQVTATLENGDSMQQTVTYVPNVANAEISLAASKDPVIANNNDLTTLTATV
                     ADTEGNAIANSEVTFTLPEDVRANFTLGDGGKVVTDTEGKAKVTLKGTKAGAHTVTAS
                     MAGGKSEQLVVNFIADTLTAQVNLNVTEDNFIANNVGMTRLQATVTDGNGNPLANEAV
                     TFTLPADVSASFTLGQGGSAITDINGKAEVTLSGTKSGTYPVTVSVNNYGVSDTKQVT
                     LIADAGTAKLASLTSVYSFVVSTTEGATMTASVTDANGNPVEGIKVNFRGTSVTLSST
                     SVETDDRGFAEILVTSTEVGLKTVSASLADKPTEVISRLLNAKADINSATITSLEIPE
                     GQVMVAQDVAVKAHVNDQFGNPILNESVTFSAEPPEHMTISQNIVSTDTHGIAEVTMT
                     PERNGSYMVKASLANGSSYEKDLVVIDQKLTLSASSPLIGVNSPTGATLTATLTSANG
                     TPVEGQVINFSVTPEGATLSGGKVRTNSSGQAPVVLTSNKVGTYTVTASFHNGVTIQT
                     QTIVKVTGNSSTAHVASFIADPSTIAATNSDLSTLKATVEDGSGNLIEGLTVYFALKS
                     GSATLTSLTAVTDQNGIATTSVRGAITGSVTVSAVTTAGGMQTVDITLVAGPADASQS
                     VLKNNRSSLKGDFTDSAELHLVLHDISGNPIKVSEGLEFVQSGTNAPYVQVSAIDYSK
                     NFSGEYKATVTGGGEGIATLIPVLNGVHQAGLSTTIQFTRAEDKIMSGTVLVNGANLP
                     TTTFPSQGFTGAYYQLNNDNFAPGKTAADYEFSSSASWVDVDATGKVTFKNVGSKWER
                     ITATPKTGGPSYIYEIRVKSWWVNAGDAFMIYSLAENFCSSNGYTLPLGDHLNHSRSR
                     GIGSLYSEWGDMGHYTTEAGFHSNMSWSSSPANSNEQYVVSLATGDQSVFEKLGFAYA
                     TCYKNL"
     gene            complement(11117..12169)
                     /locus_tag="ACHOUK_003499"
     CDS             complement(11117..12169)
                     /locus_tag="ACHOUK_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFP9898875.1"
                     /translation="MFLVSLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEAMRKKNLKTMLFISQLKAKTNFQVVGLTMKCYSPVCKNASRMDQ
                     KLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIALNLEGSSQ
                     DRTFSLSIAENLIEKIQSETDIPIVIVHGPKGEDKARVLVDCYNNVYRLSLSPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            12483..13799
                     /gene="shiA"
                     /locus_tag="ACHOUK_003500"
     CDS             12483..13799
                     /gene="shiA"
                     /locus_tag="ACHOUK_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFP9898876.1"
                     /translation="MDSTLISTRPDERTVSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQYNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRA"
     gene            13901..15355
                     /gene="amn"
                     /locus_tag="ACHOUK_003501"
     CDS             13901..15355
                     /gene="amn"
                     /locus_tag="ACHOUK_003501"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFP9898877.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT         4227 a         3681 c         3713 g         3837 t
ORIGIN      
        1 cgttaccagg caaaatacgt ttcagccatc tggcaaaaac tgctcccgag tcaggctgat
       61 ttttaatgtt caacccggat cgccgctaac ggcatatagt tagcggcggt tgtgagatat
      121 agctatgagc agcatatttt taaccgaaaa tgaattgcaa acgttaacgg gctgcaaata
      181 tgcaagccac cagcgcaact ggttaataaa aaatgggctt ccgttctata ccaatcgtag
      241 tggaaaaccg atcgtcagtc gggaactgtt tacctgtata aaaactttac caccacgtga
      301 ggctgaacct gatttcggtg cgatctaatg ggaagacgaa ggaaaaatcc cgaacacgaa
      361 aaattaccgc caaaggtata ccccaataaa tatagtgtat ggaaaccgac atccagagaa
      421 tctgtaacct taactgcaat cgaggatggt ttagccgcat tatggaaaaa gtatgaagaa
      481 acggttaacc atcgcgatcg cgcaatgaca tttgggcgtt tgtgggaaaa atttctcgcc
      541 agcgcctatt acagcgagct tagtcctaga actcaaaaag attatctgca acatcaaaaa
      601 aagctgctgg ccgtattcgg taaggtgctg gccgattctg taaaaccaga gcacatcaga
      661 cgatacatgg acaaaagagg cgagcagagt aaaacgcagg caaaccatga aaaaagcagt
      721 atgtcgcgcg tttatagttg ggggtatgag cgaggatacg tgaaggctaa cccatgtgca
      781 ggtgtaagta aattcaaggc caaaaaccgc gaacgatatg taaccgacaa agaataccag
      841 gcagtattaa gcgttgcacc tcttcctgtt tttatcgcaa tggaaattgc ctatctgtgt
      901 gcagcgaggg tttccgatgt gttatcgctg aaatgggagc agattggaaa cgacgggatc
      961 tttatccagc aagggaaaac agggaaaaaa cagataaaag catggagtcc acgattacag
     1021 gcggcgatcg aaaaagcaaa acagttacca acatccgcct atgtaatcag caatcaatac
     1081 ggcaaccgat atatgtacaa aggctttaac gaaatgtggg tagaagcaag aaatcacgca
     1141 ggcaaaattt caggtatttt aaccgacttc acctttcatg atctgaaggc gaaaggaatt
     1201 tcagactatg aaggaagcag ccgggataag caacttttct ctggtcacaa aaccgaaggg
     1261 caagtgctaa tctatgacag gaaggttaaa gtttcaccaa cacttgatgt cccgttacct
     1321 gaaaatattc caagaaaata ttccaagtaa ttccaagtgt gatttttgtc actgacttaa
     1381 tgatgtgtaa gtgattgaat tttggcggag agagggggat ttgaaccccc ggtggagttg
     1441 cccccactcc ggttttcgag accggtccgt tcagccgctc cggcatctct ccgttcagat
     1501 ggttgccatg atgccaggaa atttggcatt ttaacagtcc ctgtccgtgc aattttgttc
     1561 aagtgacgag tttgcgagca aaacgatgat taagtggccc tggaaagtac aagaatcagc
     1621 acatcaaact gcccttccct ggcaggaagc actatcgatc ccccttttaa cgtgtctgac
     1681 ggaacaggaa caaagcaaat tagtcactct tgccgaacgt tttttacagc aaaagcggct
     1741 tgttccttta cagggctttg aactggattc attaagaagc tgccggatag cacttctatt
     1801 ttgcctaccc gttctggagt taggactgga atggctggat ggttttcatg aagtcttaat
     1861 ttatcctgcg ccatttgtgg tcgatgatga atgggaagac gatatcggtc tggtgcataa
     1921 ccaacgtatt gttcagtcag gtcagagctg gcagcaaggg cctatcgttt tgaactggtt
     1981 ggatatacaa gattcttttg atgcttctgg ttttaacctg attattcatg aagtcgctca
     2041 taagctggac acccgtaacg gcgatcgcgc cagcggagtt ccctttattc cgttgcgtga
     2101 ggttgctggc tgggaacacg atcttcatgc tgcaatgaac aacattcagg aagaaatcga
     2161 attggttggc gagaatgcgg cgagcattga tgcttatgct gccagtgatc ctgctgaatg
     2221 ttttgccgta ctttctgaat atttctttag cgccccagaa ctttttgctc ctcgtttccc
     2281 ttcattgtgg caacgtttct gccaatttta tcaacaagat cctttgcaga gactgcatca
     2341 cgctaatgat acagactcgt tttcggcgac gaatgttcat taattaacaa ctttgcagat
     2401 taattaacca attgaaatga cttatgaaat ttagtgttga cagacaaggt accgctaagt
     2461 aatatgcgcc ccgttcacac gattcctctg tagttcagtc ggtagaacgg cggactgtta
     2521 atccgtatgt cactggttcg agtccagtca gaggagccaa attctaaaaa ttcgcttttt
     2581 tagcgcaatg tcactgacct tagttgaaca ttgtttttta acggatagcg ggtttttaac
     2641 atcttaagcg ccctcgacct ttatggttga gggcgttttg ctatgaacgc catcaccatt
     2701 ttcccctcga ttataaaact tgagttattc agtagtctcc cctcttgcaa ctcacatcca
     2761 aaactgccta acgaaaagtt attaattttc aatcatattg ctatcaggat ttacattttt
     2821 tcgctgtgct agaaagggcg catttatgtt agctcgttca gggaaggtaa gcatggctac
     2881 gaagaagaga agtggagaag aaataaatga ccgacaaatc ttatgcggga tgggaattaa
     2941 actacgccgc ttaactgcgg gtatctgcct gataactcaa cttgtgttcc ctatggctgc
     3001 ggcagcacaa ggtgtggtaa acgccgcaac ccaacaacca gttcctgcac aaattgccat
     3061 tgcaaatgcc aatacggtgc cctacaccct tggagcgctg gaatcggccc aaagcgttgc
     3121 cgaacgtttc ggtatttcgg tggctgagtt acgcaaactc aaccagtttc gtacgtttgc
     3181 tcgaggtttt gataatgtcc gccagggtga tgaactggat gtcccggcac aagttagtga
     3241 aaataattta accccgccac cgggtaatag cagtggcaac cttgagcaac agatagccag
     3301 tacttcacag caaatcgggt ctctgctcgc cgaggatatg aacagcgagc aagcggcaaa
     3361 tatggcgcgt ggatgggcct cttctcaggc ttcaggcgca atgacagact ggttaagccg
     3421 cttcggtacc gcaagaatca cgctgggcgt ggatgaagat tttagcctga agaactccca
     3481 gttcgatttt ctccatccgt ggtatgaaac gcctgataat ctctttttca gtcagcatac
     3541 tctccatcgt actgacgagc gtacgcagat taacaacggc ttgggttggc gtcatttcac
     3601 tcccacatgg atgtcgggca tcaacttctt tttcgaccac gatcttagcc gttaccactc
     3661 ccgcgccggc attggcgcgg agtactggcg cgactatcta aaattaagca gtaacggcta
     3721 tttgcgactg accaactggc gcagcgcacc tgaactggac aacgattatg aagcacgccc
     3781 ggccaatggc tgggatgtac gcgcagaagg ctggctaccc gcctggccgc accttggcgg
     3841 taaactggtc tatgaacagt attatggcga tgaagtggcc ctgttcgata aagatgatcg
     3901 gcaaagtaat cctcatgcca taaccgctgg acttaactat acccccttcc cgctgatgac
     3961 cttcagcgcg gagcaacgcc agggtaaaca gggcgaaaat gacacccgtt ttgccgtcga
     4021 ttttacctgg caacctggaa gcgcgatgca gaaacagctt gacccgaatg aagtcgatgc
     4081 acggcgtagc cttgcaggca gccgttttga tctggtggat cgcaacaaca acatcgttct
     4141 ggaatatcgc aaaaaagaac tggttcgcct gaccctgaca gaccccgtga cagggaagtc
     4201 aggagaagtg aaatcactgg tttcgtcgct acaaaccaaa tatgccctga aaggctataa
     4261 cgtcgaagcc accgctctgg aagctgccgg tggtaaagtg gttacaacgg gtaaagatat
     4321 tctggttacc ctgccggcgt accggttcac cagtacgcca gaaaccgata acacctggcc
     4381 gattgaagtc accgctgaag atgtcaaagg caatttttcg aatcgtgaac agagcatggt
     4441 agtcgttcag gctcctacgc taagccagaa agattcctcg gtatcgttaa gtagccagac
     4501 gttgagcgcg gattcccatt caaccgccac actgactttt attgcgcatg atgcagcagg
     4561 taatcctgtt atcgggctgg tgctctcgac gcgtcacgaa ggtgttcagg acatcaccct
     4621 ttctgactgg aaagataatg gtgacggaag ctatacccag atcctgacca caggagcgat
     4681 gtctggcacg ctgacgctga tgccacagct gaacggtgtg gatgcggcta aagcccccgc
     4741 cgtggtgaat atcatttctg tttcgtcatc ccggactcac tcgtcaatta agattgataa
     4801 ggaccgttat ctctccggga atcctatcga ggtgacggta gaactgagag atgaaaatga
     4861 caaacctgtt aaggagcaaa aacagcaact gaataccgca gtcagcatcg acaacgtgaa
     4921 acctggtgtc actacagact ggaaagaaac cgcagatggc gtctataagg caacctatac
     4981 cgcctatacc aaaggcagtg ggcttactgc gaagctgtta atgcaaaact ggaatgaaga
     5041 tttgcatacc gctggattta tcatcgacgc caacccgcag tcagcgaaaa ttgcgacatt
     5101 atctgccagc aataatggtg tgctcgccaa tgagaatgca gcaaacaccg tctcggtcaa
     5161 tgtcgctgat gaaggaagca acccaatcaa tgatcatacc gtcacgtttg cggtattaag
     5221 cggatcggca acttccttta acaatcaaaa caccgcaaaa acggatgtta atggtctggc
     5281 gacttttgat ctgaaaagta gtaagcagga agacaacacg gttgaagtca cccttgaaaa
     5341 tggcgtgaaa caaacgttaa tcgtcagttt tgtcggcgac tcgagtaccg cgcaggttga
     5401 tctgcagaag tcgaaaaatg aagtggtcgc tgacggcaat gacagtgcca caatgaccgc
     5461 gacagttcgg gatgcaaaag gcaacctgct caatgacgtc aaggtcacct tcaatgtcaa
     5521 ttcagcagca gcgaaactga gccaaaccga agtgaatagc cacgacggga tcgccacagc
     5581 tacgctgacc agtttgaaaa atggtgatta tacggttacg gcctctgtga gctctggttc
     5641 tcaggctaat caacaggtga tttttatcgg tgatcaaagt actgctgccc tgaccctcag
     5701 tgtgccttca ggtgatatca ccgtcaccaa cacagctccg ctacatatga ctgcaacctt
     5761 gcaggataaa aatggcaatc cactaaaaga taaagaaatc accttctctg tgccaaacga
     5821 cgtcgcaagt cggttctcga ttagcaacag cggaaaaggc atgacggata gcaacgggac
     5881 tgcaatcgcc tccctgaccg gcacgttagc gggcacgcat atgatcacgg ctcgtctggc
     5941 taacagcaat gtcagcgata cacagccaat gacgtttgtg gcggataaag acagagcggt
     6001 tgtcgttctg caaacatcga aagcggaaat cattgggaat ggcgtggatg agacgactct
     6061 gacagcaaca gttaaagatc cttttgataa cgtggttaaa aatctttcag tagtcttccg
     6121 cacctccccc gcagacacgc aactgagtct gaacgcgcgt aatactaatg agaacggtat
     6181 tgccgaagtt acccttaagg gcacggtttt gggtgttcat acagccgaag ccatactgct
     6241 taacggcaac agagatacga aaatcgtcaa tattgcgccc gatgccagca acgcgcaggt
     6301 caccctgaac atccctgcac aacaggtggt gacgaataac agtgacagcg tgcagctgac
     6361 ggcgacggtg aaagacccgt cgaatcatcc ggtggcggga ataacggtga acttcaccat
     6421 gccacaggac gtggcggcaa actttaccct tgaaaataac ggtattgcca tcactcaggc
     6481 caatggcgaa gcgcatgtca ccctcaaagg caaaaaagcg ggcacgcata ctgtgaccgc
     6541 cacgctgggt aacaataatg ccagcgatgc gcaaccagtc accttcgtgg cggataagga
     6601 cagcgcggtt gtcgttctgc aaacatcgaa agcggaaatc attgggaatg gcgtggatga
     6661 gacgactctg acggcaacag tgaaagatcc ttttgataac gcagtaaaag atctacaggt
     6721 caccttcagt accaaccccg cagatactca acttagtcag agcaaaagca atactaacga
     6781 cagtggtgtg gccgaagtta cctttaaggg cacggttttg ggtgttcata cagccgaagc
     6841 cacactgcct aacggcaaca acgatacgaa gatagtcaat attgcgcccg atgccagcaa
     6901 cgcgcaggtt acgctgaaca tccctgctca acaggtggtg acgaataaca gcgacagcgt
     6961 gcagctgacg gcgacggtga aagatccgtc gaatcatccg gtggcgggaa taacggtgaa
     7021 cttcaccatg ccacaggacg tggcggcaaa ctttaccctc gaaaataacg gtattgccat
     7081 cacccaggcc aatggggaag cgcatgtcac gctcaaaggt aaaaaagcgg gtacgcatac
     7141 ggttaccgca acgctgagta ataacaatac cagtgattca cagccggtaa cgtttgtggc
     7201 ggacaaaacc tcggctctgg ttgttcttca gatatcaaaa aatgagatca caggtaatgg
     7261 cgtcgatagc gcaacgctaa ctgcaacggt caaagatcag ttcgacaatg aggtgaacaa
     7321 tcttccggta acattcagca cagcttcttc aggcctcacc ctgaccccag gggaaagtaa
     7381 taccaatgag tctggcatcg cgcaggccac tctcgcaggc gttgcctttg gtgagcagac
     7441 ggtcactgca tcactggcta ataatggtgc cagcgacaac aaaactgtgc attttattgg
     7501 cgacacagcg gcggcaaaaa ttatcgagtt gacgcctgtc ccagacagca taatcgcagg
     7561 taccccgcag aacagctccg gcagcgtcat caccgccaca gtcgttgata ataatggctt
     7621 tccggtgaaa ggtgtgactg tgaacttcac cagcaacgca gcgacagccg aaatgacgaa
     7681 tggcggtcaa gccgtgacga acgaacaggg taaggctacc gtcacttata ccaatacccg
     7741 ctcctcgata gaatcaggag cgagaccgga taccgttgag gccagtctgg aaaatggtag
     7801 ctccacgctt agcacatcaa ttaatgtcaa cgctgatgcg tctacggcac atctcacctt
     7861 gctacaggca ctttttgata cagtctccgc aggcgacact accaatctgt atattgaggt
     7921 gaaggataat tacggcaacg gagtacccca gcaggaggta accctcagcg tttcaccaag
     7981 tgaaggtgtg acccccagta ataacgctat atatacgacc aatcacgacg gcaattttta
     8041 cgcaagcttt accgctacaa aagccggggt ataccaagtg acggcaaccc tcgaaaatgg
     8101 cgattcgatg caacaaacag tgacctatgt gccgaacgta gcgaatgctg aaatctcgct
     8161 ggcagcctcg aaggatccgg taattgccaa caataacgat ctcacgacac taacagcaac
     8221 agtcgctgat acagagggca atgcgatagc caacagtgag gtaacattta ctctgccgga
     8281 agatgtgagg gcgaacttca cgctgggcga tggcggtaaa gtggttactg atactgaagg
     8341 caaagcgaaa gtcacgctga aaggtacaaa agcaggcgct catactgtta cagcatcgat
     8401 ggctggcggt aagagtgagc agttggtggt gaactttatt gcggatacac tcactgcgca
     8461 ggttaatctt aacgttaccg aggacaattt tatcgctaat aacgtcggga tgaccaggct
     8521 gcaggcaaca gtgactgatg gaaacggcaa cccgttagcc aatgaggcgg tgacattcac
     8581 gctaccggca gatgtgagcg caagctttac tctcggacaa ggcggttccg ccattactga
     8641 catcaacggc aaggctgaag ttacactgag cggtacaaaa tccggcacct accccgtgac
     8701 agttagcgtg aacaattatg gtgtcagtga tacgaaacag gtgactttga ttgccgatgc
     8761 tggtaccgca aaactagcct ccttaacctc tgtatactca ttcgtcgtca gcacgaccga
     8821 gggcgcgacc atgactgcaa gcgtcactga cgctaacggc aacccggtag aaggtataaa
     8881 agttaatttc cgcggaactt ccgtcacgct aagcagcacc agcgttgaaa cggatgatcg
     8941 gggtttcgct gaaattcttg tgacaagcac cgaggtcgga ctgaaaacag tttcagcctc
     9001 tctggcagat aaacctactg aagtcatctc gcgattactg aatgcaaaag cagatattaa
     9061 ttctgcaacg attaccagtc tggagatacc tgaaggtcag gtcatggtcg cacaagacgt
     9121 agcagttaaa gctcacgtca acgaccagtt tggcaatccg attcttaatg aatctgtaac
     9181 attcagtgca gaaccaccag agcacatgac catcagccaa aatattgtct ctactgatac
     9241 gcatggtata gccgaggtca ctatgacgcc cgaaagaaac ggttcgtata tggtgaaagc
     9301 atccctggcg aatggatcct cttatgagaa ggatctggtg gtaatcgatc aaaaactgac
     9361 actctcggcg tccagcccgc ttatcggtgt caattcccca acaggtgcaa ctctgacggc
     9421 aacgctaact tctgcaaatg gcactccagt ggagggtcag gtcatcaact ttagcgtaac
     9481 gccagaaggt gcgacgttaa gtggcggaaa agtgagaacc aactcttcag gtcaggctcc
     9541 agtcgttctg accagcaata aagtcggtac atatacggtg actgcatcgt tccataacgg
     9601 cgtaacaata cagacacaga caatcgtgaa agtcactggc aactcaagca ccgcccatgt
     9661 tgctagcttt atcgctgatc catcgactat agccgccacc aacagtgatt taagtacctt
     9721 aaaggcaacg gttgaggatg gcagtggtaa cctgatcgaa ggtctcactg tgtacttcgc
     9781 cttaaaaagc ggctctgcca cattaacgtc attaacagcg gtgacagatc aaaacggaat
     9841 cgcgacaaca agcgtgagag gagcgataac ggggagcgtc acggtaagcg cagtcacgac
     9901 cgctggtgga atgcaaacag tagatataac gctggtggca ggcccggcag acgcctcgca
     9961 gtccgtcctt aagaacaatc ggtcatcatt gaaaggagac tttaccgata gtgctgagct
    10021 acatcttgtt ctgcacgata tatcaggcaa tccgatcaaa gtttctgaag ggctggaatt
    10081 tgtgcagtca ggtaccaacg cgccctatgt gcaagttagt gcaattgact acagtaaaaa
    10141 tttctcaggc gagtacaaag ccactgttac aggcggcgga gagggtatcg caacgctgat
    10201 ccctgtattg aatggtgttc atcaagcggg tctgagtacc acaatacaat tcactcgcgc
    10261 agaagacaaa ataatgagcg gtacagtgtt agtcaatggt gctaacctac cgacaactac
    10321 attcccttcg caggggttca ctggggcgta ttatcagttg aataatgaca actttgcccc
    10381 aggaaaaacg gcggctgatt atgagttttc aagctctgcc tcctgggttg atgttgatgc
    10441 taccggtaaa gtgacattta aaaatgtcgg cagcaaatgg gagaggatta cggcgacgcc
    10501 aaaaacaggc ggccctagct atatatacga aatccgagtg aagagttggt gggtgaacgc
    10561 cggcgatgct ttcatgatat acagccttgc tgaaaatttt tgcagtagca atggctacac
    10621 acttcccctt ggagaccatt taaaccatag tcgttcccga ggcatcgggt cactgtacag
    10681 tgaatgggga gatatggggc attacacgac tgaagctggt tttcattcaa atatgtcttg
    10741 gtcatcgagt cccgcaaact caaacgaaca atacgtagtt tccctggcaa caggtgatca
    10801 aagcgtattt gaaaagcttg ggtttgctta tgcgacatgt tataaaaacc tctgattttc
    10861 cgttataatt tcttaaagag tgctccattc ggggcactct tttttacatt ccctctatag
    10921 agcttgccct tctctcacag aaaatgaata aataaaaatg cgataccgca agaatcgcgt
    10981 tcgatgtgac tatgatcgta tccggtgagt acagctttta tccaaaggct ggttttctgt
    11041 gatgattgac cagcctcaga gtattgatga caccaccgtt cataacagat cggcattact
    11101 atgcataagt actgtctcat attctcgcca aaacactttt taatgccttt gcgaattcat
    11161 ccagacttat attgtcaatt ttttgcccaa cgacgactga ctccgaaaca tcggccattg
    11221 cgggccatcg cgttttgtaa tcagcataaa ttgcaacaac gggggtatta taggcacttg
    11281 ccatatgtaa tattgaggtg tcaggagtta ttgcgatata agcatctttt atgattgctg
    11341 ctgaacgttt aatcgagggt gataaggata aacggtagac attattataa caatccacta
    11401 ataccctggc tttatcttcc ccttttggtc catgaacgat cactattggt atatctgttt
    11461 cactttgaat tttttctatt agattttctg caatcgatag tgaaaatgta cgatcctgcg
    11521 agctaccttc taaattaagc gcaatgtaag atcctaacga tcgcatttcc tcgcgcacct
    11581 catcgagtac atcctcactt agtggcaatt catatattgg cctgactgct ggaaaacccg
    11641 cctcacgcat cataaatgcc catgtcatgg gtacgggagc ccggagtttc tgatccatcc
    11701 gggacgcgtt cttacacaca ggggaatagc acttcatggt taaaccaaca acctgaaaat
    11761 tcgtttttgc tttcaactga ctgataaata acatcgtttt caggttcttt ttacgcatcg
    11821 cctctataca aagatccggc gtaccgtatt gctgtgttat gttcttcgct aaatctttta
    11881 tttcttttaa tgttgcgtga tcctgcatag tcattgtgac taatgttaat ttagtctgtt
    11941 caagtttgag cgcattaaag acttctaaat taattgtcga cgtaacaatt aatagatgct
    12001 taattttatg caattcaagc gcccgaataa caggaaagat ggccatagca tcgccaatct
    12061 gatcgggaat atggatgaca acaaagtctg ttttttcaat attgaaatta taagctttat
    12121 aatcgtagta actaaatgca atacgtctca acaatgatac taaaaacata actaacctcg
    12181 cctccctact ggttataatg caatgcagtc tatcagaatc atcagggtgc aattttgtgc
    12241 atatgcggac ttttatgttt catatctcga acctgtgggg cctctgctta atccttaaac
    12301 aacaccagca actcctgcgc tttcatcttc cagcgaattt ttcatgttgc cgttaatcag
    12361 ccataaaatc atttgcagat gcgctctgtc gaggtagtct cataaggttc gtttatagat
    12421 cgacgcaatg tgagttacct tttccatact aattataaaa agacagtaca aacaggatca
    12481 ttatggactc cacgctcatc tccactcgtc ccgatgaaag gacggtttcg ttaagtcgcg
    12541 cccgacgagc tgcgttaggc agtttcgctg gtgccgtcgt cgactggtat gattttttac
    12601 tctatggcat taccgccgca ctggtgttta atcgcgagtt tttcccgcaa gtaagcccgg
    12661 ctatgggaac gctcgccgca tttgccacct ttggcgtcgg attccttttc cgtccgctcg
    12721 gcggtgtcat tttcggtcac tttggcgacc gactgggacg taagcgcatg ttaatgctga
    12781 ccgtctggat gatgggcatc gcgacagcct tgattggtat tcttccttca ttctcgacca
    12841 ttgggtggtg ggcacctatt ttactggtga cgctgcgtgc cattcaggga tttgccgtcg
    12901 gcggcgaatg gggaggcgcg gcgttgcttt ccgttgaaag tgcaccgaaa aataaaaaag
    12961 ccttttacag tagcggtgta caagttggct acggtgtagg tttactgctt tcaaccggac
    13021 tggtttcatt gatcagtatg atgacgactg acgaacagtt tttaagctgg ggctggcgca
    13081 ttcctttcct gtttagcatc gtactggtac tgggagcatt gtgggtgcgc aatggcatgg
    13141 aagagtccgc ggaatttgaa caacagcaat ataatcaagc ggccgcgaaa aaacgcatcc
    13201 cggttatcga agcgctgtta cgacatcccg gtgctttcct gaagattatt gcgctacgac
    13261 tgtgcgagtt gctgacaatg tacatcgtta ccgcctttgc acttaattat tcaactcaga
    13321 atatggggtt accgcgcgaa cttttcctta atattggttt gctggtaggt ggattaagct
    13381 gcctgacaat tccctgtttt gcctggcttg ccgatcgttt tggtcgccgc agggtttata
    13441 tcacaggcgc gttgatcgga acgttgagcg catttccttt ctttatggcg cttgaagcac
    13501 aatctatttt ctggatagtt ttcttctcca taatgctggc aaacattgcg catgacatgg
    13561 tggtgtgtgt gcaacaaccg atgtttaccg aaatgtttgg tgccagttat cgctatagtg
    13621 gtgctggagt cggttatcag gttgccagtg tggttggcgg tggatttaca ccttttattg
    13681 ccgctgcgct catcacttac tttgccggga actggcatag cgtcgccatt tatttgctgg
    13741 ctggatgtct gatttccgca atgaccgctt tgttgatgaa agacaatcaa cgcgcttgat
    13801 agtctggcga agatcatccg atcttcgcct tacacttttg tttcacattc ctgtgacata
    13861 ctatcggatg tgcggtaatt gtatggaaca ggagacacac atgaataata agggctccgg
    13921 tctgacccca gctcaggcac tggataaact cgacgcgctg tatgagcaat ctgtagtcgc
    13981 attacgcaac gccattggca actatattac aagtggcgaa ttacctgatg aaaacgcccg
    14041 caaacaaggt ctttttgtct atccatctct gaccgtaacc tgggacggta gcacaacaaa
    14101 tccccccaaa acgcgcgcat ttggtcgttt tactcacgca ggcagctaca ccaccacgat
    14161 tactcgccct actctctttc gttcgtatct taatgaacaa cttacgttgc tgtatcagga
    14221 ttatggcgcg catatctcgg tgcaaccctc acagcatgaa atcccttatc cttatgtcat
    14281 cgatggctct gaattaacac ttgatcgctc aatgagcgct gggttaactc gctatttccc
    14341 gacaacagaa ctggcgcaaa ttggcgatga aactgcagac ggcatttatc atccaactga
    14401 attctccccg ctatcgcatt ttgatgcgcg ccgcgtcgat ttttccctcg cgcggttgcg
    14461 ccattatacc ggtacgccag ttgaacattt tcagccgttc gtcttgttta ccaactacac
    14521 acgttatgtg gatgaattcg ttcgttgggg atgcagccag atcctcgatc ctgatagtcc
    14581 ctacattgcc ctttcttgtg ctggcgggaa ctggatcacc gccgaaaccg aagcgccaga
    14641 agaagccatt tccgaccttg catggaaaaa acaccagatg ccagcatggc atttaattac
    14701 cgccgatggt cagggtatta ctctggtgaa tattggcgtg ggaccgtcaa atgctaaaac
    14761 catctgcgat catctggcag tgctacgccc ggatgtctgg ttgatgattg gtcactgtgg
    14821 cggattacgt gaaagtcagg ccattggcga ttatgtactt gcacacgctt atttacgcga
    14881 tgaccacgtt cttgatgcgg ttctgccacc cgatattcct attccgagca ttgctgaagt
    14941 gcaacgtgcg ctttatgacg ccaccaagct ggtgagcggc aggcccggtg aggaagtcaa
    15001 acagcggcta cgtactggta ctgtggtaac cacagatgac aggaactggg aattacgtta
    15061 ctcagcttct gcacttcgtt ttaacttaag ccgggccgta gcaattgata tggaaagtgc
    15121 aaccattgcc gcgcaaggat atcgtttccg cgtgccatac gggacactac tgtgtgtttc
    15181 agataaaccg ttgcatggcg agattaaact tcccggtcag gctaaccgtt tttatgaagg
    15241 cgctatttcc gaacacctac aaattggcat tcgggcgatc gatttgctgc gcgcagaagg
    15301 cgaccgactg cattcacgta aattacgaac ctttaatgag ccgccgttcc gataataagt
    15361 tctgaacctt cttatcaaaa atagatgtga aggattaagt aagacatctg gcaaatagcc
    15421 cgcaataaac gcgggctgtt tgcttccttg ggcggata
//
LOCUS       DAZKIP010000102        15296 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZKIP010000102 DAZKIP010000000
VERSION     DAZKIP010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.103"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            81..329
                     /locus_tag="ACHOUK_003502"
     CDS             81..329
                     /locus_tag="ACHOUK_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFP9898878.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPSTLSKYAKAGMIFPLPKKVGRR
                     WRVDPQARFVGMVNKPEVIATDHPALKRILEDGAPAKI"
     gene            307..1437
                     /locus_tag="ACHOUK_003503"
     CDS             307..1437
                     /locus_tag="ACHOUK_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFP9898879.1"
                     /translation="MARPRKYKTDVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKLAETIAAEANSRLARQQMEQMLSLQEKIISDTGGSSTVTIFLNNYRKIQQERYENG
                     EIKLNTLKQKAAPLRVFDERFGTRPLDAITVKDVVSVLEEYKARGHNRMGQIFRKVLI
                     DVFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGML
                     LALMTGQRLSDICKMQFSDIRDGYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSC
                     RDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQR
                     SLSERLFREQGVDTKILLGHSNQKMTDIYNDARGKEWKKLVI"
     gene            complement(1483..2121)
                     /gene="ompW"
                     /locus_tag="ACHOUK_003504"
     CDS             complement(1483..2121)
                     /gene="ompW"
                     /locus_tag="ACHOUK_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFP9898880.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            2361..2450
                     /locus_tag="ACHOUK_003505"
                     /pseudo
     CDS             2361..2450
                     /locus_tag="ACHOUK_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            2478..3221
                     /gene="yciC"
                     /locus_tag="ACHOUK_003506"
     CDS             2478..3221
                     /gene="yciC"
                     /locus_tag="ACHOUK_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFP9898881.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGVFASIRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            3251..3790
                     /gene="yciB"
                     /locus_tag="ACHOUK_003507"
     CDS             3251..3790
                     /gene="yciB"
                     /locus_tag="ACHOUK_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFP9898882.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3895..4293
                     /gene="yciA"
                     /locus_tag="ACHOUK_003508"
     CDS             3895..4293
                     /gene="yciA"
                     /locus_tag="ACHOUK_003508"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFP9898883.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATDALFKYVAVDPEGKPRALPVE"
     gene            complement(4333..5052)
                     /gene="tonB"
                     /locus_tag="ACHOUK_003509"
     CDS             complement(4333..5052)
                     /gene="tonB"
                     /locus_tag="ACHOUK_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFP9898884.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQQKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            5276..5572
                     /gene="yciI"
                     /locus_tag="ACHOUK_003510"
     CDS             5276..5572
                     /gene="yciI"
                     /locus_tag="ACHOUK_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFP9898885.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            5873..7126
                     /gene="kch"
                     /locus_tag="ACHOUK_003511"
     CDS             5873..7126
                     /gene="kch"
                     /locus_tag="ACHOUK_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFP9898886.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(7181..7354)
                     /gene="yciY"
                     /locus_tag="ACHOUK_003512"
     CDS             complement(7181..7354)
                     /gene="yciY"
                     /locus_tag="ACHOUK_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFP9898887.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            7497..8957
                     /gene="cls"
                     /locus_tag="ACHOUK_003513"
     CDS             7497..8957
                     /gene="cls"
                     /locus_tag="ACHOUK_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFP9898888.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFHSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKVYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            8992..9321
                     /gene="yciU"
                     /locus_tag="ACHOUK_003514"
     CDS             8992..9321
                     /gene="yciU"
                     /locus_tag="ACHOUK_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFP9898889.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(9374..10378)
                     /gene="oppF"
                     /locus_tag="ACHOUK_003515"
     CDS             complement(9374..10378)
                     /gene="oppF"
                     /locus_tag="ACHOUK_003515"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFP9898890.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(10375..11388)
                     /gene="oppD"
                     /locus_tag="ACHOUK_003516"
     CDS             complement(10375..11388)
                     /gene="oppD"
                     /locus_tag="ACHOUK_003516"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFP9898891.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(11400..12308)
                     /gene="oppC"
                     /locus_tag="ACHOUK_003517"
     CDS             complement(11400..12308)
                     /gene="oppC"
                     /locus_tag="ACHOUK_003517"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFP9898892.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(12323..13243)
                     /gene="oppB"
                     /locus_tag="ACHOUK_003518"
     CDS             complement(12323..13243)
                     /gene="oppB"
                     /locus_tag="ACHOUK_003518"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFP9898893.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(13329..14960)
                     /gene="oppA"
                     /locus_tag="ACHOUK_003519"
     CDS             complement(13329..14960)
                     /gene="oppA"
                     /locus_tag="ACHOUK_003519"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFP9898894.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
BASE COUNT         3882 a         3744 c         3635 g         4035 t
ORIGIN      
        1 tcaggctgat ttttaatgtt caaccccggt cgttgcccac cagctatagt ggcggcgacc
       61 atgattagcg aacgacgctc atggcaagac ttattctgct cactgagtgg gcaaaagagg
      121 aattcagtga accggtccca actccgagta cgttaagtaa atacgctaaa gccggaatga
      181 tatttcctct ccccaaaaaa gttggaagac gctggcgagt ggatccgcaa gctcgctttg
      241 tcggaatggt aaacaagccg gaggtgatcg ccacagatca ccctgctttg aagaggatac
      301 tggaagatgg cgcgcccgcg aaaatataaa accgatgttc cgggattatc tccgtatttt
      361 gacaaaagaa ataacaaagt ttactggcgt tacaggcatc ccataacagg caaaaatcac
      421 ggtctcggca gtattgacca gaaactggca gaaactattg cagcagaagc gaacagccgt
      481 cttgcccggc agcaaatgga acaaatgctc agtctgcagg agaaaattat tagtgatacc
      541 ggcggttcat caaccgttac catttttctg aataattaca gaaaaattca acaggaaaga
      601 tatgaaaacg gcgagatcaa actcaacacg ctgaaacaga aagcggcccc tctcagggta
      661 tttgatgaac gttttggcac cagaccgtta gatgccataa ccgtaaaaga tgtggtatca
      721 gtactggaag agtacaaggc cagaggacat aacagaatgg gacaaatttt caggaaagta
      781 ctgatcgatg ttttccggga agctcagcaa acgggcgatg tcccgccagg ctttaaccct
      841 gcagaatcgg caaaaaaacc gcaggtgcgg atatcaagac agcgactgac ttttgatgag
      901 tggatgatga tttataacgc agcggaaaag gatggttact ttttacagcg cggtatgctg
      961 ctggcactga tgacaggcca gcgcctttca gatatttgca aaatgcaatt ttcggatatc
     1021 cgggatggtt atcttcatgt cgaacagcaa aaaacaggaa cccggattgc catccctctg
     1081 gctctgcgtt gcgataaatt aaatctcacc ctggatgatg tggtgtcatc ctgccgcgat
     1141 tgcgttctta gtccgtggct attgcaccac catcacgcga aagggacagc taagcgcggc
     1201 gggatggtta agccagcaac attaaccgtt gcatttaaaa aagcccggga ttctgtggat
     1261 tacaactggc gtgctaatgg cacccctccc tctttccatg agcagagatc tttatcagag
     1321 cgattgttca gagagcaggg ggttgatacc aaaattttgc taggccattc gaatcaaaaa
     1381 atgaccgata tttacaacga cgcacgcggt aaggaatgga aaaaactggt catttgatga
     1441 ccagttttgc agaggggttt tgcagaggtt ttgcagagaa aattaaaaac gatatcctgc
     1501 ggagaacata aacacccacg gatccagtcg taccgagtct tttacggtag taacaccaga
     1561 tttgtactta gccgtggtat cgatatccat gtaccacact gacatgttaa ccaaccagtc
     1621 acggttaatc agataatcaa cccccacctg cccggcagct ccccaggaat ctttcagact
     1681 gagatcggaa agccccgcct ctttgccatg atcgttaaat ccattatcaa agaaggtggt
     1741 gtagttaata cctgccccaa cataaggacg gaatttgctg ctggcatcac caaaatacca
     1801 ctgcgccatc agtgttggcg gcagatgatg aacggttgca atatcgccgg tcgcccgggt
     1861 gccgatttta tggcggaacg gcgtcgctgc cagtaattcc acaccaatgt tgtcggtcgc
     1921 catataagta aacgtcaggc ccagttgagt gttattggtc acgctgaatc cacccagact
     1981 tcctaacgta ccaccagcac cttctgttgg acgtacggtt gcagaacctg cacgcataaa
     2041 aaattcgcct gcttcatgcg caaaggcact gccagagaga agagttgtta ctgccaaagc
     2101 cgccactgtt aactttttca tatccgctcc gtcgttatgg ttataaaaac gtgacgaata
     2161 tacctacatt ggagtaacaa atgatcctac atagatcaca ttacatatga taattcacca
     2221 tttattgatc tggattaatt tagaagttgc attgagaatc ctggttaacg tgatttaaat
     2281 caatttttaa atattttcgc tacgcaaata ttaactcgtt cgccacttgc cgcggtttgc
     2341 ccacgcctga aggccagcaa ttacctccgc tttacaaaga tatgcttttc catacaagac
     2401 ctgatgctgc cacccgaggc ttaaccaggg tacaattgcc cgctaattaa cacctgcata
     2461 aaactcaagg agagtgcatg tctatcacgg cgcagtccgt ataccgtgac accggaaatt
     2521 tcttccgtaa tcaatttatg accattctgt tggtatcgtt gctatgtgcg tttatcacag
     2581 tggtgttagg gcatgttttc tcacccagtg atgcacagct tgcgcagctc aatgacggcg
     2641 tgcccgttag cggcagtagt gggttgttcg acctggttca gaatatgtca ccggaacagc
     2701 aacaaatttt gctgcaggct tcagcggcgt ccactttttc aggattaatc ggtaacgcca
     2761 ttctcgccgg aggcgtaata ttaattatcc agctggtgtc tgcgggtcag agagtcagtg
     2821 cgctgcgagc tattggtgcc agtgcgccga tattgccaaa gttatttatt ctgatttttc
     2881 tgactaccct tttagtacag attggcatca tgctggtggt cgttccggga attatcatgg
     2941 ccatattact ggccctggca ccggtgatgc tggtacagga caaaatgggc gtttttgcct
     3001 cgatccgtag cagtatgcgg ctgacttggg cgaatatgcg tctggtggca cccgcagtac
     3061 tgagctggtt gctggcaaaa acactgttgc tgctttttgc ctcttctttt gccgcattaa
     3121 ccccggaaat tggtgccgta ctggcgaaca ccttgagcaa cctgatttca gccatattgc
     3181 tcatctatct gttccgcctg tatatgttga ttcgccaata accttaagca tttgattacg
     3241 gaatcgtaaa atgaagcagt ttcttgattt tttaccgctg gttgtctttt tcgcgtttta
     3301 caaaatttat gacatctatg cggctactgc ggcgctgatc gtcgccacgg cgattgtgct
     3361 tatctatagc tgggttcgct ttcgtaaggt tgagaagatg gccctgatca cttttgttct
     3421 ggtggtcgtc ttcggtggct tgacgctgtt cttccacaat gatgagttta ttaaatggaa
     3481 ggttacagtc atttatgctc tgtttgcggg agccctgtta gtcagccaat gggtgatgaa
     3541 aaagccgcta attcagcgga tgctgggtaa agaactcacg ctgccgcaac cggtatggtc
     3601 gaagctgaat ctggcctggg ctgttttctt tatcctttgc ggtctggcaa acatctacat
     3661 cgcattctgg ctgccgcaaa atatttgggt caactttaaa gtctttggcc tgaccgccct
     3721 taccttaatc ttcacattgt taagcggtat ctatatctac cgccacatgc cgcaggaaga
     3781 taaatcctaa ccagactgcc agtcctttgg ggctggcgct gtatctcctc tgttctcctc
     3841 aggaaaatca tagtagcatc gcgcctgtga ttttcctttt aagtcggttt taccatgtct
     3901 acaacacata acgtccctca gggcgatctt gttttacgta ctttagccat gcccgccgat
     3961 accaatgcca atggtgacat ctttggtggt tggttaatgt cacaaatgga tattggcggc
     4021 gctattctgg caaaagaaat tgcccacggt cgcgtagtga ctgtgcgggt tgaaggaatg
     4081 actttcttac ggccggttgc ggtcggcgat gtggtgtgct gctatgcacg ctgtgtccag
     4141 aaagggacga catcggtcag cattaatatt gaagtgtggg tgaaaaaagt agcgtctgaa
     4201 ccaatcgggc aacgctataa agcgacagac gcattattta agtatgtcgc ggttgatcct
     4261 gaaggaaaac ctcgcgcctt acctgttgag taatagtcaa aagcctccgg tcggaggctt
     4321 ttgactttct gcttactgaa tttcggtggt gccgttaatt ttaaacagga tattcaccac
     4381 aatcccactg cctggcttac ccggctcata acgccatctg cgcatcgcat ttttcacctc
     4441 acgctcaaac atgttcgcag gcttggctga gaggatttgt acgttatcca cgcgaccatc
     4501 cggcgtgacg tcaaatttaa ctttaacctg cccttcaatg cgcaatgcct gtgctcgtgc
     4561 cggatactgc ggctgattac ggcttaatgc gcgtggtcct gaagccacac tggtaaccgg
     4621 cttgctggtt gcagccgttg ctgtacttga tgtcgggcgt gccggtgccg tattttcaaa
     4681 cggtgatgcc ggacgcgact ctacgggttt gacatcgcgt ttttgctgct cctgtacctt
     4741 tttcaccggc ttcggttttg gcttaggttt cggcttcggc ttttcaatga ccaccggtgc
     4801 ttcttttggc ggttcgggga tcggctcagg ttccggttct ggctctacca ccggctccgg
     4861 tggcggctga acggcttgtg gcggttcgag atcagcaggc gcaaccatcg tgacagaaat
     4921 cggctgcgca ggcgcaggta gttcaataac ctgatgtacc gaggtataga gcagacccgc
     4981 cacaacagca ccatgaatgc agaccgaaag taacgtcggc caggggaagc ggcgaggtaa
     5041 atcaagggtc attgaagtca taatcatttc agtagaaaaa ccaggtctcg attttaaatg
     5101 caaatagcaa tcatattcaa taaggcaaga cgatcaggat cagtgaaaat gtcatttttg
     5161 gcgtaattga ccttacttta tgcatggtct taacaaagac atcatctttc cgttgcagat
     5221 cttcgccctt tcaaataacg tactttacaa ctttcccgaa caaggagttg tgcccgtgtt
     5281 gtatgttatt tacgctcaag acaaagctga tagcctcgaa aaacgccttt ccgttcgtcc
     5341 ggcacattta gcacgtttac agttactgca tgatgaaggt cgtttgttga cagctggtcc
     5401 aatgccagcg gtagacagta acgatccagg ggctgcggga tttactggct caaccgttat
     5461 tgctgaattt gaatcgctgg aagcagctca ggcctgggcc gatgctgacc cgtacgtagc
     5521 ggcaggagtc tatgaacacg tttcagtgaa accatttaag aaagtgttct gagtagagat
     5581 ccaatattta agggcacggt ttttgcaacc gtgttcttgt ttggcctttc cttataggtc
     5641 ggtcattgca ttcaccacca caagcagata ataactttat ctgtatttct ttatatgatg
     5701 tgagcatgtt caggaaaaat gccctttcct gacgcagagt tgctcactca aaacctttga
     5761 ttactgcgga taaagtcatc tactattgtt taacacattc acgtgtcgca tgtatgttca
     5821 gaaaatgtgc acgttaagat aattaaacaa aattgtggtt acagaggtat cagtgagtca
     5881 ctgggctaca ttcaaacaaa cagcaacaaa tttatgggtg accctacggc acgacattct
     5941 cgcgctggcc gtctttttaa atgggttgct tatttttaaa acaatctatg gtatgtcggt
     6001 caatttgctt gatattttcc atatcaaagc attttcagag ttggatctct ccttgctggc
     6061 aaacgcccca ctatttatgc tcggcgtctt tcttgtcctg aactccattg gcttactgtt
     6121 ccgggcaaag ctcgcatggg caatcagtat cattttgttg ttgatagcgc taatttacac
     6181 cctgcatttt tatccctggc tgaaatttag tattggattt tgcattttta cgctggtgtt
     6241 tttactgata ctgcgcaaag acttctccca cagtagcgcc gcagccggga caatttttgc
     6301 atttattagt ttcacgacat tactgtttta ctcaacctac ggtgcgcttt atttaagcga
     6361 aggttttaat ccgcgaatag aaagtttgat gaccgcgttc tatttttcga tagaaaccat
     6421 gtcaaccgtc ggctatggcg atattgtccc tgtttctgaa tcagcacgat tgttcactat
     6481 ttcggtcatt atttccggca ttaccgtttt tgccacatct atgacctcaa tttttggtcc
     6541 gcttatccgc gggggattca acaaacttgt aaaaggaaac aatcatacaa tgcatcgtaa
     6601 agatcatttt attgtttgcg gacattcgat tctcgccatt aatacgatcc tgcaactgaa
     6661 tcaacgcgga caaaacgtaa cggttatcag caacttgcct gaagatgata tcaagcaact
     6721 tgagcaacgc ttaggcgata acgctgatgt tatccccggt gacagtaatg acagttctgt
     6781 attaaagaaa gcgggaatcg atcgttgccg ggccattctg gcgctgagtg ataacgatgc
     6841 agataacgcg tttgttgtac tctcggcaaa agatatgagc agtgatgtca aaacagttct
     6901 cgccgtcagt gatagcaaaa acctgaataa gattaagatg gtacatccgg atatcattct
     6961 ctcaccgcaa ctgtttggca gcgaaattct ggcgcgagtt ttaaatggtg aagagattaa
     7021 taatgatatg ctcgtttcaa tgttgttgaa ctcaggtcat ggtattttca gcgataacga
     7081 tgaacaagaa acgaaagctg acagtaaaga atcggcgcaa aaatagagtt taaagacgga
     7141 agagtaaact tattgggcac cgcaatggtg cccagtacat ttagatattg tagatcgcaa
     7201 acaataacga gttacgctgt ttgtttagaa tgcacttcct gatgctgtgg atacgcatat
     7261 ttcggcgcga ctgcccctca agccaacgcg atttacgtcg gctagcctga cgctgcatgc
     7321 gccagcgccc cacttccgtt ctactccgct tcatgttaac tactctatgc aataacagaa
     7381 tggtcattat acccccgccc ccgacgctga ccagtgcttt tcccgcgttt ttatttgcca
     7441 gatgaatcca tatgcgtaaa ctcataacaa tgcgctttca aaaggatttc taatctatga
     7501 caaccgttta tacgttggtg agttggttgg ccattctggg atactggttg ctcattgcag
     7561 gcgtaacttt acgcattcta atgaaacgac gcgcagttcc ctccgcgatg gcctggctgt
     7621 tgattattta cattctgccg ttagtcggaa ttattgccta tcttgccgtt ggcgagctcc
     7681 atttaggcaa acgccgcgct gagcgcgcca gagcgatgtg gccttccacc gcaaaatggc
     7741 ttaacgacct taaagcctgt aagcatatct ttgccgaaga aaatagcagt gtcgctgcgc
     7801 cattattcaa gctttgcgag cgtcgtcagg ggatcgctgg ggtcaaaggg aatcagctac
     7861 aactgatgac cgagtcagat gatgtgatgc aggcgttaat ccgcgacatc cagctcgcgc
     7921 gccataatat tgagatggtg ttttatatct ggcagcccgg cggcatggcg gaccaggtgg
     7981 ctgaatcatt aatggcggct gcgcgacgcg gcattcattg ccgattgatg ctcgactccg
     8041 ccgggagtgt agcttttttc cacagcccgt ggcccgagct aatgcgtaat gccggtattg
     8101 aagtggtcga agccttaaag gtcaatctga tgcgtgtgtt tttacgccgt atggacctgc
     8161 gccaacatcg caagatgatc atgatcgata attacatcgc gtacaccggc agcatgaata
     8221 tggtcgaccc tcgctacttc aaacaagatg cgggcgtagg gcaatggatt gatctgatgg
     8281 cgcgtatgga aggccccatc gccaccgcga tggggattat ttattcctgc gactgggaga
     8341 ttgaaacagg gaaacgtatt ctaccgccac cacctgatgt caatattatg ccgtttgagc
     8401 aggccagcgg tcataccatt cacacaattg cttctggccc cggctttccg gaagatctca
     8461 ttcaccaggc attattgact gcggcttatt cggcgcgtga atatttgatc atgaccacgc
     8521 cctactttgt gccaagcgat gatttacttc atgcgatttg cacggcggcg cagcgcgggg
     8581 tggatgtcag tattatcctt ccgcgaaaaa atgactcgat gctggtcggc tgggccagtc
     8641 gcgcattctt tacggaactg cttgctgctg gggttaaagt ttatcagttt gaaggcgggt
     8701 tactgcatac caagagcgtg ctggtcgatg gcgaactaag tctggttggc accgttaacc
     8761 ttgatatgcg tagtctgtgg ctgaatttcg agattaccct ggcaatcgac gataaaggtt
     8821 ttggtgcaga ccttgccgcc gttcaggacg attatatttc gcgttcacgt ctgctcgatg
     8881 cccgtttatg gctaaaacgt ccattatggc aacgtgtcgc cgagcgactg ttttacttct
     8941 tcagcccgtt gctgtaaaac gtcgccaaca gaggttaaac aggtagtgac tatggatatg
     9001 gatctaaaca atcgcctgac tgaagatgaa acgcttgagc aggcctacga tatttttctc
     9061 gaactggctg ccgacaatct cgatccagct gatgttctgc tgttcaatct tcagtttgaa
     9121 gagcgcggcg gcgcggagtt attcgatccg gcggaagact ggcaggaaca tgttgatttc
     9181 gatttgaacc cagacttttt tgccgaagtt gtgattggtc tggcggacag tgaagatggt
     9241 gaaatcaacg atgttttcgc ccgcattctg ttatgccgcg aaaaagatca caaactttgc
     9301 catatcatct ggcgggaata aataatcact ttcggcgtga aaaagggctg acagctgtca
     9361 gccctttttg tgtttaaagc ggatcgactt tcaggcaaga aacggcgtgt ctgaaactcc
     9421 cctccagcac aggacgtgtt ttggcacact ccggaccggc aatcgggcaa cgggtacgga
     9481 aaacacaacc ggaaggcggg ttgatcggcg acggtaattc cccttccagt aactggatgg
     9541 ttttgttctt ctccagatcc ggatcaggta tggggactgc cgacatcaat gccctggtgt
     9601 aaggatgtag tggattgtgg tagacctcat cataggtccc cagttctacc gcatggccga
     9661 gatacatcac caacacacga tcggaaatgt gttttaccac ggccaggtca tgagcgataa
     9721 aaattaatga caatcccatc tcacgttgca attgctggag caggttgacc acctgtgcct
     9781 gaattgacac gtccagcgcc gacaccggct catcacagat aatcagcttc ggttcaagaa
     9841 taagagcacg cgcaatcccg atacgctggc actgcccacc agagaactca tgcggatagc
     9901 ggttaatcag gttaggcaat aacccgactt tcagcatcat cgccttcacg cgctcgcgaa
     9961 cttcctggcg tgacattttc ggatgataag tacgcagtgg ttcagcgatg atctcgccga
    10021 tggtcatacg cgggtttagc gatgccaacg gatcctggaa aatcatctga atatcactgc
    10081 gaacggcacg ccattcatcg ggtttcatgc ccagcaactc tttacctaac caggcaacat
    10141 gaccgtcggt cgctttaacc aggccaatga tggcgcgagc aaaggtggac ttaccgcatc
    10201 ccgattcccc taccacacct aatgtttccc cttcatacag gcgaagagtt acaccatcta
    10261 cggctttgag cgttttcggc ggttgccaga accactgttt gccatctttg atttcaaagt
    10321 gcactttcag atcggcgatt tcgaggagga cttttcttcc ttcggttaca gcattcataa
    10381 cagttcctcc accggtttaa agcaagcacg cagacggcca ggcgtaaact cttccagcgg
    10441 cggtgcgcta ctacaaattt ccatcgcatg cggacaacgt ggctggaatg ggcaaccttt
    10501 cggtaatcgc agcaggtttg gcggattacc cggaatagtc aacattgttt caccttccgc
    10561 atcgagacga ggcaccgcgt tgagcaaacc gatagaataa ggatgaacgg gttgataaaa
    10621 gacatcgcgc gcgttgccat attccatcgt gcgcccggcg tacattacca gcactttgtc
    10681 gcagatcccc gccaccacgc caagatcgtg ggtgatcata atgatggcgg tattaaattc
    10741 ccgcttcagt tcattcaata gcgtcatgat ctgcgcctgt acggtgacgt ccagcgcagt
    10801 agtgggttca tccgcaatca gcagcttagg tcgacatagc aatgccatcg caatcataac
    10861 tcgctgacgc atgccgccag aaaattcgtg cgggtacatt ttcatgcgtt tacgtgcttc
    10921 cggcattttt accgcatcga gcatccgcac cgactcttca aacgcctcag ctttgctcat
    10981 gttcttatgc agcatcagca cttccatcaa ctgctcaccg acgcgcatat agggattcaa
    11041 cgaagtcatt gggtcctgaa aaatcattga gatttgttca gcgcgcagtt tattgagttc
    11101 acgctctggc aaattgagga tttcacgccc attgaaggtt gccgatccgc caatgcgtcc
    11161 gttggcagcc aacaggccca tcaacgcaaa tgcagtttgc gatttacccg aaccagactc
    11221 acctacaatg cccagcgttt ctccggcacg tagggaaaaa tttaaatcat tgaccgccgt
    11281 gacgtcgccg tccggggtac taaaggtgac acgcaaatct ttcacgttca gcagtgcgtc
    11341 agcctgttgt tgtgcgagcg gcacagttgc agtttcaatt acgctcatgg ctgcactcct
    11401 taacgatctt tcgggtcgag ggcatcacgc aagccatcgc cgataaagtt gaaacaaaac
    11461 agcgtcacca cgaggaatcc cgctgggaac aacagtaacc atggagagac ttccattgag
    11521 ttcgcgccat cactcagcaa tgccccccag ctgcttaacg gctcttgcgt acccaacccc
    11581 aggaagctaa ggaaagattc aaagaggatc atgctgggca ccagtagcga tgcgtagacc
    11641 accaccacac cgagtacgtt cggcacaatg tggcgaataa caatgcccga cgtcgataca
    11701 ccgccaactt gtgccgcctc aataaactct ttgcgcttca gactcagggt ttgcccacgc
    11761 acaatacgag ccatatccag ccaggaaacc atgcctatcg ccacgaaaat cagcaggatg
    11821 ttttgaccga aaaaggtcac cagcaaaatg acgaagaaca tgaatgggaa ggagttgagg
    11881 atttccagca aacgcatcat taccgaatcc actttaccgc ccagataacc ggaaagcgaa
    11941 ccataaagtg tccccacgac cactgccacc agtgccgcag caacaccgac catgagtgag
    12001 atacgtccgc caatcgcaac gcgcacaagc aggtcgcgac cggatgagtc agtaccaaag
    12061 tagtgaccgg actccatatc cggggcgctg gacatcatcg cccagtcagt atcgtcatag
    12121 gcaaactgcg aaagcatcgg tgccaggatt acaaataacg cgatcagcac cagcactatc
    12181 agacttgcaa ccgccgcacg gttatgcata aaacgtcgac gtgcgtcctg ccacaagctg
    12241 cgcccttcga cctccagctt ttcactgaaa ttttccagcg tctcgctgtt tttcttactt
    12301 aacatcatcg cgagctccag tatcagtaac ggattttcgg gtcgataacc gcatatagca
    12361 catcgacaat ggcattaaac aaaatggtta acgcaccaac caggatggtc aggcttaaca
    12421 ctaaggaata gtcacggttc aatgcgccat taacgaacag ttgcccaatc cccggcaaac
    12481 cataaatggt ttcgataacc atagaaccgg taataatgcc gacaaatgcg gggcccatat
    12541 aggagagcac gggtaacaga gcaggtttta atgcgtggcg taaaatgatc cgccgcatag
    12601 gtaacccttt cgcccgggca gtacgaataa agttggagtg taatacttca atcatagagc
    12661 cacgggtaat acgcgcaata ctggcgatat aggcgagtga caacgccacc atcggcaata
    12721 tcatgaattt aagcgcccca ccattccagc caccgcccgg cagccaatgc aaaatgatcg
    12781 caaatatcat gactaataat ggcgcaacca caaaactggg gataacaacc ccggtcattg
    12841 ccagccccat cacggtatag tcccatttgg tgttttgttt taatgcggca ataacgccag
    12901 cactaacacc cagtattacc gcaaggaaaa atgctgcggc tcccagtttg gcagaaacgg
    12961 gaaaactgga tgcaaccagg tcattgaccg aataatcttt atatttaaac gatggaccga
    13021 aatcgccatg cgccagttgt ttcaggtagc tgaaatactg tgtcatgatc ggatcattaa
    13081 gatgatattt cgcttcgata ttggccatta cttccggcgg taaagtacgt tcgccggtaa
    13141 aagggcttcc cggcgcgagg cgcatcataa agaacgaaat agtaataaga ataaatagcg
    13201 tcggaatcgc ttccagacag cgacgtagaa taaattttaa cattgcccta ccttctggcc
    13261 tgtgccttct gtaatgcgat aaaaatcaga caccgtggag caggacactc ctgccccacg
    13321 tattgccatc agtgcttcac aatgtacata ttccgggtgt aggtattatc cagcggatct
    13381 ttgccggtat aaccaccaac ccacggtttc accagacgcg cattcacgta gtaataaaca
    13441 ggaacaatgg ccgaatcctt atccagctgt tgttctgctt tagtgtacag agctgtgcgc
    13501 tgcgcctcgt cagtcacttt cagcgtttcc gccataatgc tgtcaaaggc cgggctctta
    13561 taatgcgcgg tattcatcga gctgttcgac agcatggtgt tcaggaagga agttggttcg
    13621 ttgtagtcag cacaccagcc tgcacgggcc acatcaaaag taccctggtg acgggtgtcg
    13681 aggaacgttt tccactcctg gttaaccagt ttgacgttta caccaatgtt tttcttccac
    13741 aatgaagagg cagcaatcgc cagcttttta tgcagatcgg aggtgttata caacaggttg
    13801 atggtcaacg gtttgtctgc ggtataaccc gcttcagcca gcagtttttt cgcttcttcg
    13861 ttacgttttt cctggctcca gccaaaccat tccggctgag tcaattttgc gccatcagta
    13921 tacggtggag tgtaaccata ggcgggcatg tcgccctgcg ctttcacttt attaacaatg
    13981 atatcgcggt ccatacccag tttcagtgcg gtacgcacac gcacatcgtt gaatggcggt
    14041 ttctggttgt taatttcgta ataataagtg cacaggtatg gatcaacgtg aacttcgtcc
    14101 gggatctctt ttttcagctt ctggaacaat tcgatcggca tgttgttata agtcatgtcg
    14161 atttcaccac tacggtagcg gttgacatcg gtaacttcag aagcaatggg caaataggtt
    14221 acctggttaa taacggtttt cgcgttgttc cagtaggttg ggctgcgttc aagaacgatt
    14281 cgttcgttta cgacccaatc ttttaaggta taggcaccgt tggtgacgat attaccaggc
    14341 tgggtccatt tttcgccgaa tttctcgata gcggcttttg gcaccggtga agttgatggg
    14401 tgaacaagta atttatagaa gtacggaacg ggttcactta aggtgacttc taatgtgtga
    14461 tcatcaatag ctttcacgcc gagatcggta atcggttttt tcccttcaag aatttcatca
    14521 ataccggcga tatgcccata ttgcagataa ctggcatacg gagaagcggt gttcggatca
    14581 acagaacgtt gccagctata cacaaagtct tgtgctgtga ctggcgtgcc atcagaccat
    14641 ttcgcatctt tacgcaaatg gaaggtccag actttcgcgt ctttattatc ccaggattca
    14701 gcgacgccag gtgccggatg gccgtcaaga tcgctgacca gtaagccttc aaacaggtct
    14761 cggctgatat tagactccgg aacaccttca attttgtgcg gatctaatga ctgaacttct
    14821 gaaccattgt tacgtaccag cgtttgtttt tccgccagtg tgacgcctgc gggtacatca
    14881 gctgccagcg cgacattccc tgccattagc gcagccagaa cgccagctgc tactaaactt
    14941 ctcttggtga tgttggtcat tgttttttgg actccctcat tataattact ggtattaacc
    15001 agcatgtgta atcccggatt gggaccctgc acaggctcgg agactttctg ctgtcaggtt
    15061 actttatcgt tacggattgc tatcaccgac tttatttatt ctggtgattc agacgatcac
    15121 cttatgtcga ttcgtactgt gtctcccctg tcgagacaca ttattctttc gcgacgctct
    15181 ttaattgtta attattctca ttaccaataa agaatcgcct gacggttgcc cggaaagtac
    15241 caaatgcggt tcttactcgc caatacattt tgcaaaattg ttaagcaatt ctcttt
//
LOCUS       DAZKIP010000103        15090 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZKIP010000103 DAZKIP010000000
VERSION     DAZKIP010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15090
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.104"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            4..981
                     /gene="ybeQ"
                     /locus_tag="ACHOUK_003520"
                     /pseudo
     CDS             4..981
                     /gene="ybeQ"
                     /locus_tag="ACHOUK_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
     gene            complement(1051..1533)
                     /gene="ybeL"
                     /locus_tag="ACHOUK_003521"
     CDS             complement(1051..1533)
                     /gene="ybeL"
                     /locus_tag="ACHOUK_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFP9898895.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            1769..4351
                     /gene="leuS"
                     /locus_tag="ACHOUK_003522"
     CDS             1769..4351
                     /gene="leuS"
                     /locus_tag="ACHOUK_003522"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFP9898896.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVNTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPQWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEKQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            4366..4947
                     /gene="lptE"
                     /locus_tag="ACHOUK_003523"
     CDS             4366..4947
                     /gene="lptE"
                     /locus_tag="ACHOUK_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFP9898897.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGAVSISQDTASVFRNGQTAEYQMVM
                     TVSASVLIPGRDIYPISTKVFRSFFDNPQMALAKDNEQEMIIKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            4947..5978
                     /gene="holA"
                     /locus_tag="ACHOUK_003524"
     CDS             4947..5978
                     /gene="holA"
                     /locus_tag="ACHOUK_003524"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFP9898898.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYSQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            5980..6621
                     /gene="nadD"
                     /locus_tag="ACHOUK_003525"
     CDS             5980..6621
                     /gene="nadD"
                     /locus_tag="ACHOUK_003525"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFP9898899.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCDDLLPEPVLTYINQQGLYR"
     gene            6645..7256
                     /gene="cobC"
                     /locus_tag="ACHOUK_003526"
     CDS             6645..7256
                     /gene="cobC"
                     /locus_tag="ACHOUK_003526"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFP9898900.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            7516..7833
                     /gene="rsfS"
                     /locus_tag="ACHOUK_003527"
     CDS             7516..7833
                     /gene="rsfS"
                     /locus_tag="ACHOUK_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFP9898901.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            7837..8304
                     /gene="rlmH"
                     /locus_tag="ACHOUK_003528"
     CDS             7837..8304
                     /gene="rlmH"
                     /locus_tag="ACHOUK_003528"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFP9898902.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            8335..10236
                     /gene="mrdA"
                     /locus_tag="ACHOUK_003529"
     CDS             8335..10236
                     /gene="mrdA"
                     /locus_tag="ACHOUK_003529"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFP9898903.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            10239..11351
                     /gene="mrdB"
                     /locus_tag="ACHOUK_003530"
                     /gene_synonym="rodA"
     CDS             10239..11351
                     /gene="mrdB"
                     /locus_tag="ACHOUK_003530"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFP9898904.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            11362..12450
                     /gene="rlpA"
                     /locus_tag="ACHOUK_003531"
     CDS             11362..12450
                     /gene="rlpA"
                     /locus_tag="ACHOUK_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFP9898905.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFIQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FANKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            12590..13801
                     /gene="dacA"
                     /locus_tag="ACHOUK_003532"
     CDS             12590..13801
                     /gene="dacA"
                     /locus_tag="ACHOUK_003532"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFP9898906.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            13911..14174
                     /gene="ybeD"
                     /locus_tag="ACHOUK_003533"
     CDS             13911..14174
                     /gene="ybeD"
                     /locus_tag="ACHOUK_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFP9898907.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            14275..14916
                     /gene="lipB"
                     /locus_tag="ACHOUK_003534"
     CDS             14275..14916
                     /gene="lipB"
                     /locus_tag="ACHOUK_003534"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFP9898908.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
BASE COUNT         3716 a         3825 c         3938 g         3611 t
ORIGIN      
        1 ataatgattt tcacgtcaag ttgctgcgat aatttatcaa tagatgagat tatcgaacgt
       61 gctgaaaaag gcgattgcga ggctcaatat attgttgggt tttattataa tcgcgatagc
      121 gcaattgact ctccggacga cgaaaaggcc ttttactagc tgaagctggc cgctgagcaa
      181 ggtcattgtg aagcacagta ttccttaggg caaaagtata ccgaggataa aagccgtcat
      241 aaagataatg agcaagccat cttctggctg aaaaaagctg ccctacaagg ccatactttc
      301 gcttccaacg cccttggctg gatactggat cgtggagaag accccaacta taaagaagcg
      361 gttgcctggt atcagatagc cgcggagagc ggaatgtctt atgcgcaaaa taatcttggg
      421 tggatgtaca gaaatggcaa cggagccgca caagactata cgctggcatt tttctggtac
      481 aaacaagctg cattacaagg ccatagtgac gcgcaaaaca atctggccga tctttatgaa
      541 gacggaaaag gcgttgctca aaacgagaca ctcgccgcat tctggtattt gaaaagcgca
      601 cagcagggta atcggcatgc tcagtttcaa attgcatggg attataacgc tggcgaaggg
      661 gtggaccagg actataagca agcgatgtac tggtatctga aggctgccgc tcaggggagc
      721 gtcggcgctt acgtcaacat cggttatatg tataaacacg gacaaggcgt tgagaaagat
      781 tatcaggctg cctttgaatg gtttacgaaa gccgctgaat gcaatgacgc cactgcctgg
      841 tataacctgg ccattatgta tcactacgga gaaggaagac ctgtcgatct ccgacaggct
      901 ctcgacctgt atcgtaaagt tcagtcatcc ggaaccagag atgtcagtca ggaaattcgt
      961 gagactgaag atttactgta gagataaaaa caagggccgg ggaatttcat tcggcccata
     1021 ttctctttat tgctgtgcga aatcagacta ttacggctca aacgggcgtc tctggaactg
     1081 gtcatgacca catttcgggc atagcgtcag cacttccggt gtgtagatcg ggagatggaa
     1141 gtgacatttc tcgcagacca gatttcccag cccgaccact tctccgctgt gataaacccc
     1201 atgatgattg aggtcctgga aaacttcgcg ccattcaagc tgcgttttat cggtgatgtc
     1261 tgccagctcc tgccacaagc tttctttaat cacccgcata aagacgctgt cagattcttc
     1321 tttcaggctc tcttcatagc tcatggcgaa ctcttccagg tcacgtctga cagctcgcgt
     1381 cagctcatcg acctcggttc gcgttaactc ccctgttttt attacgcgct cgcgcgcctg
     1441 ttccaccagt gcgtcgatat cacgttcgcc attgcgcagg cgttcgctca gtgacgcaac
     1501 cagttcacgg taatattgag caaccttgtt catcgtttca tctcccgggt cgttaactga
     1561 ctataataat agacgttctt tgttccttgc tttgctaata cgcccacaaa ctctgtaaat
     1621 taatgtatga ataatccggg catcatttca acgtcgagtg cgcgaaaggc tgttttgacg
     1681 cggacgtttg ggctatgcta tgcggatctg aaaaaccacc tctaacgcta cttttgtagc
     1741 cgtaatgaaa acaggaccac tggctgccat gcaagagcaa taccgcccgg aagagataga
     1801 atccaaagta cagcttcatt gggatgagaa gcgcacattt gaagtaaccg aagacgagag
     1861 caaagagaag tattactgcc tgtctatgct tccctatcct tctggtcgac tacacatggg
     1921 ccacgtacgt aactacacca tcggtgacgt gatcgcccgt taccagcgca tgctgggcaa
     1981 aaacgttctg cagccgatcg gctgggacgc gtttggtctg cctgcggaag gcgcggcggt
     2041 gaaaaacaac accgctccgg caccgtggac gtacgacaac atcgcgtata tgaaaaacca
     2101 gctcaaaatg ctgggctttg gttatgactg gagccgcgag ctggcaacct gtacgccgga
     2161 atactaccgt tgggaacaga aattcttcac cgagctgtat aaaaaaggcc tggtatataa
     2221 gaagacttct gcggtcaact ggtgcccgaa cgaccagacc gtactggcga acgaacaagt
     2281 tatcgacggc tgctgctggc gctgcgatac caaagttgaa cgtaaagaga tcccgcagtg
     2341 gtttatcaaa atcactgctt acgctgacga gctgctcaac gatttggata aactggatca
     2401 ctggccagac accgttaaaa ccatgcagcg taactggatc ggtcgttccg aaggcgtgga
     2461 aatcaccttc aacgttaacg actatgacaa cacgctgacc gtttacacta cccgcccgga
     2521 cacctttatg ggttgtacct acctggcggt agctgcgggt catccgctgg cgcagaaagc
     2581 ggcggaaaat aatcctgaac tggcggcctt tattgacgaa tgccgtaaca ccaaagttgc
     2641 tgaagctgaa atggcgacga tggagaaaaa aggcgtcaat actggcttta aagctgttca
     2701 cccattaacg ggcgaagaaa ttcccgtttg ggcagcaaac ttcgtattga tggagtacgg
     2761 cacgggcgca gtaatggcgg ttccggggca cgaccaacgc gactacgagt ttgcctctaa
     2821 atacggcctg aacatcaaac cggttatcct ggcggctgac ggctctgagc cagatctttc
     2881 tcagcaagcc ctgactgaaa aaggcgtgct gttcaactct ggcgagttca acggtcttga
     2941 ccatgaagcg gccttcaacg ccatcgccga taaactgact gcgatgggcg ttggcgagcg
     3001 taaagtgaac taccgcctgc gtgactgggg tgtttctcgt cagcgttact ggggcgcgcc
     3061 gattccgatg gtgacgctgg aagacggtac cgtaatgccg accccggacg accagctgcc
     3121 ggtgatcctg ccggaagatg tggtgatgga cggcattacc agcccgatta aagcagatcc
     3181 acagtgggcg aaaactaccg ttaacggtat gccagcgctg cgtgaaaccg acactttcga
     3241 cacctttatg gagtcctcct ggtactatgc gcgctacact tgcccagagt acaaagaagg
     3301 tatgctggat tccgaagcgg ctaactactg gctgccggtg gatatctaca ttggtggtat
     3361 cgaacacgcc attatgcacc tgctctactt ccgcttcttc cacaaactga tgcgtgatgc
     3421 aggcatggtg aactctgacg aaccagcgaa acagttgctg tgtcagggta tggtgctggc
     3481 agatgccttc tactatgttg gcgaaaacgg cgaacgtaac tgggtttccc cggttgatgc
     3541 tatcgttgaa cgtgacgaga aaggccgtat cgtgaaagcg aaagatgcgg caggccatga
     3601 actggtttat accggcatga gcaaaatgtc caagtcgaag aacaacggta tcgacccgca
     3661 ggtgatggtt gaacgttacg gcgcggacac cgttcgtctg tttatgatgt ttgcttctcc
     3721 ggctgatatg actctcgaat ggcaggaatc cggcgtggaa ggggctaacc gcttcctgaa
     3781 acgtgtctgg aaactggtgt acgagcatac tgcgaaaggt gatgttgcgg cactgaacgt
     3841 tgatgcactg actgaagatc agaaagcgct gcgtcgcgat gtgcataaaa ctatcgctaa
     3901 agtgaccgat gatatcggcc gtcgtcagac cttcaacacc gcaattgcgg cgattatgga
     3961 gctgatgaac aaactggcga aagcaccgac cgatggcgag caggatcgcg ctctgatgca
     4021 ggaagcactg ctggccgttg tccgtatgct taacccgttc accccgcaca tctgcttcac
     4081 gctgtggcag gaactgaaag gcgaaggcga tatcgacaac gcgccgtggc cggttgctga
     4141 cgaaaaagcg atggtggaag actccacgct ggtcgtggtg caggttaacg gtaaagtccg
     4201 tgctaaaatc accgttccgg tggacgcaac ggaaaaacag gttcgcgaac gtgctggcca
     4261 ggaacatctg gtagcaaaat atcttgatgg cgttactgta cgtaaagtga tttacgtacc
     4321 aggtaaactc ctcaatctgg tcgttggcta agcgcgggag gaagcgtgcg atatctggca
     4381 acattgttgt tatctctggc ggtgttaatc accgccgggt gtggctggca tctgcgtgac
     4441 accacgcagg ttccttccac tatgaaggtc atgatcctgg actcaggcga tccgaacggg
     4501 ccattaagcc gtgcggtgcg taaccagtta cgtctgaatg gtgtcgagtt gcttgataaa
     4561 gaaaccacgc gtaaggacgt tccatccctg cgtttgggtg ctgtatcaat ctcacaggat
     4621 accgcgtcgg ttttccgtaa tggtcaaact gcggaatatc agatggtgat gactgttagc
     4681 gcgtcggtat tgattcctgg acgagatatc tacccgatta gcaccaaagt cttccgttca
     4741 ttcttcgata acccgcaaat ggcgttagcg aaagataacg agcaggaaat gatcattaaa
     4801 gagatgtacg accgtgctgc cgaacagctg attcgtaagc tgccaagcat ccgtgctgcg
     4861 gatattcgtt ccgacgaaga acagacgtcg actacaacgg atactccggc aacgcctgca
     4921 cgcgtctcca ccacgctggg taactgatga ttcggttgta cccggaacaa ctccgcgcgc
     4981 agctcaatga agggctgcgc gcggcgtatc ttttacttgg taacgatcct ctgttattgc
     5041 aggaaagcca ggacgctgtt cgtcaggtag ctgcggcaca aggattcgaa gaacaccaca
     5101 ctttttccat tgatcccaac actgactgga atgcgatctt ttcgttatgc caggccatga
     5161 gtctgtttgc cagtcgacaa acgctattgc tgttgttacc agaaaacgga ccgaacgcgg
     5221 cgatcaatga acaacttctc acactcaccg gacttctgca tgacgacctg ctgttgatcg
     5281 tccgcggtaa taaattaagc aaagcgcaag aaaatgccgc ctggtttact gctcttgcga
     5341 atcgcagcgt gcaggtgacc tgtcagacac cggagcaggc tcagcttccc cgctgggttg
     5401 ctgcgcgcgc aaaacagctc aacttagaac tggatgacgc ggcgaatcag gtgctctgct
     5461 actgttatga aggtaacctg ctggcgctgg ctcaggcact ggagcgttta tcgctgctct
     5521 ggccagacgg caaactgaca ttaccgcgcg ttgaacaggc ggtgaatgat gccgcgcatt
     5581 tcactccttt tcattgggtt gatgctttgt tgatgggaaa aagtaagcgc gcattgcata
     5641 ttcttcagca actgcgtctg gaaggcagcg aaccggttat tttgttgcgc acattacaac
     5701 gtgaactctt gttactggtt aacctgaaac gccagtctgc ccatacgcca ctgcgtgcgt
     5761 tgtttgataa gcatcgggta tggcagaacc gccggggcat gatgggcgag gcgttaaatc
     5821 gcttaagtca gccgcagtta cgtcaggccg tgcaactcct gacacgaacg gaactcaccc
     5881 tcaaacaaga ttacagtcag tcagtgtggg cagagttgga agggttatct cttctgttgt
     5941 gccataaacc cctggcggac gtatttatcg acggttgata tgaaatcttt acaggctctg
     6001 tttggcggca cctttgatcc ggtgcactat ggtcatctaa aacccgtgga aacgctggcg
     6061 aatttgattg gtctgacgcg agtcacaatc atccctaata atgttcctcc gcatcgtccc
     6121 cagccggaag cgaacagcgt acagcgtaaa cacatgcttg aactggcgat tgccgacaag
     6181 ccattattta ctcttgatga acgcgagcta aagcgcaatg ccccctctta cactgcgcaa
     6241 acactgaaag agtggcggca ggaacaaggg ccggacgtgc cgctggcgtt tattattggt
     6301 caggattcat tgctgacctt tccgacctgg tacgaatacg aaacgatact cgacaatgca
     6361 catttgatcg tctgtcggcg tccaggttac ccacttgaaa tggcgcaacc gcaataccag
     6421 caatggctgg aagatcattt gacacataac ccggaagatc ttcaccttca gcctgccggt
     6481 aaaatttatc tggctgaaac gccgtggttt aacatctcgg cgaccatcat ccgcgaacgt
     6541 ttgcaaaacg gtgaatcatg tgatgattta ttgccggaac cggtactgac ttacattaac
     6601 caacaaggct tgtatcgctg atacctgtcg ttacggaggg catgatgcga ctgtggttaa
     6661 ttcgtcatgg cgaaacacaa gcgaatgtcg atggtctgta cagcggtcat gcgcccacac
     6721 ccctgaccgc acgcggtatt gagcaagcgc aaaatctgca tacactgcta gatgatgttt
     6781 cctttgacct ggttttatgc agtgaactgg aacgggcaca gcataccgcg cgactggttc
     6841 tcagtgaccg ccagctcccc gttcatatca tccctgaact caacgaaatg ttttttggcg
     6901 actgggagat gcgacatcat cgcgacctca tgcaagaaga tgccgaaaac tatagcgcgt
     6961 ggtgcaatga ctggcagcat gctatcccca cgaacggtga aggatttcag gcattttcgc
     7021 aacgtgtgga acgctttatc gcaaggctta gtgaatatca gcactatcag aatattttaa
     7081 tcgtcagcca tcagggcgta cttagtctgt taattgctcg tttaattggc atgcctgccg
     7141 agtctatgtg gcattttcgc gttgaccagg ggtgttggag cgccattgat ataaaccaaa
     7201 aattcgcgac gctacgcgtc ctcaatagcc gtgccatcgg ggtcgagaat gcatgacttt
     7261 tctgtttttt tacgggtaag ccgcaacgac cattgacagc cccgggcagg ctgatattct
     7321 ccgcagccag actttttccg ccagacacga ctttgtagaa attgttttac aaaaatggcg
     7381 atgcaatctg cggcgcgggg tgggatgata gcccactttc gaaagccgat tcggcgacaa
     7441 ttgtcccgaa atcgcctctg gttcaggtat actgacagac catttttatc tatttgattc
     7501 acccaggggg aaaacttgca gggtaaagca ctccaggatt ttgttatcga caaaattgat
     7561 gacctcaaag gtcaggacat catcgcctta gacgttcagg gcaaatccag catcactgac
     7621 tgcatgatca tctgtacggg tacgtccagc cgtcatgtta tgtccattgc tgaccacgtt
     7681 gtgcaggagt ctcgcgcagc gggcctgtta ccgctcggag tagaaggtga aaacagcgcc
     7741 gactggattg tcgtggattt gggcgatgtg attgtccatg tcatgcagga agagagccgt
     7801 cgcctgtatg aactggaaaa actctggagt taatgcgtga agctgcaact tgtcgccgtg
     7861 ggaacgaaaa tgccggactg ggtacaaacc ggttttaccg agtacctgcg tcgttttccg
     7921 aaagatatgc ccttcgagct gattgaaatt ccggccggaa aacgcggcaa gaatgcggac
     7981 atcaagcgca tactcgacaa agagggtgag cagatgttgg cggccgcagg caaaaaccgc
     8041 attgtcaccc tcgatattcc aggcaagccc tgggatacgc cgcagttagc cgctgagctg
     8101 gaacgctgga agctggatgg tcgcgacgtc agtctactga ttggcgggcc tgaagggttg
     8161 tcgcctgcct gtaaagcggc ggcggagcag agctggtcgc tgtcggcgct taccctcccc
     8221 catccgctgg ttcgcgtgct ggtcgcagag agtctgtacc gggcgtggag catcaccacc
     8281 aaccatcctt atcaccgtga gtgataaggg agctttgagt agaaaacgca gcggatgaaa
     8341 ctacagaact cttttcgcga ctatacggca gagtccgcgc tgtttgtgcg ccgggcgctg
     8401 gtcgcctttt tggggatttt gctgctgacc ggcgtgctta tcgccaacct gtataatctg
     8461 caaattgttc gctttaccga ctaccagacc cgctctaatg aaaaccgcat taagctggtg
     8521 cctatcgcgc ccagccgcgg cattatctac gaccgtaacg gtatccctct ggccctcaac
     8581 cgcactatct accagataga aatgatgccg gagaaagtcg ataacgtgca gcaaacgctg
     8641 gacgctttgc gcagcgtggt tgatctgacc gatgacgata ttgctgcatt ccgaaaagag
     8701 cgcgcacgtt cacaccgttt cacctccatt ccggtgaaaa ctaacctgac cgaagtacaa
     8761 gtagctcgct ttgccgtcaa tcagtaccgt tttccgggtg ttgaagttaa aggctataaa
     8821 cgtcgttact atccttacgg ttcggcgttg acccacgtca tcggctatgt gtcgaaaatc
     8881 aacgataaag acgtcgaacg cctgaataat gacggcaaac tggccaacta tgcggcaacg
     8941 catgatatcg gtaagctggg cattgagcgt tactatgaag acgtgctgca cggtcagacc
     9001 ggttatgaag aggttgaagt taacaaccgt gggcgtgtta tccgccagtt aaaagaagtc
     9061 ccaccgcaag ccggacacga tatttacctg acgctggatc tcaaactcca gcaatatatt
     9121 gaaacgctgc tggcgggtag ccgcgcagct gtggtagtca ccgatccgcg tacaggtggg
     9181 gtgctggcgc tggtttccac gcctagttat gacccaaact tgtttgttga cggtatctcc
     9241 agcaaagatt attccgcctt gttgaacgac ccgaatacac cgctggtgaa ccgcgccaca
     9301 cagggggttt atcctcccgc gtctacagtt aaaccctatg tggcggtttc ggcattgagc
     9361 gccggggtga tcacgcgcaa tacgacgctg tttgaccctg gctggtggca actgccaggt
     9421 tcggaaaaac gttatcgtga ctggaaaaaa tggggccacg ggcgtctgaa tgtcacaaga
     9481 tcgctggaag aatctgcgga taccttcttc tatcaggtgg cctacgatat ggggatcgat
     9541 cgcctctccg aatggatggg taaattcggt tatggtcatt ataccggtat cgacctggcg
     9601 gaagaacgtt ccggcaatat gcctacccgc gaatggaaac agaaacgctt taaaaaaccg
     9661 tggtatcagg gtgacaccat tccggttggt atcggtcagg gttactggac agcgacccca
     9721 atccagatga gtaaggcact gatgatcctg attaatgacg gtatcgtgaa ggttccgcat
     9781 ttgctgatga gcaccgccga agacggcaaa caggtgccat gggtacaacc gcatgaaccg
     9841 cccgttggcg atattcattc cggttactgg gagctggcga aagacggtat gtacggtgtt
     9901 gctaaccgcc ctaacggtac ggcgcataaa tactttgcca gcgcaccgta caaaattgcg
     9961 gcgaaatctg gtaccgctca ggtcttcggt ctgaaagcga acgaaaccta taatgcgcac
    10021 aaaattgccg agcgtttacg tgaccacaaa ctgatgaccg cctttgcgcc atacaacaat
    10081 ccgcaagtgg ctgtcgccat gattctggag aacggtggcg cgggtccggc ggttggtacg
    10141 ctgatgcgcc agatcctcga ccacattatg ctgggtgata acaacaccga tctgcctgcg
    10201 gaaaatcctg cggttgccgc agcggaggac cattaatcat gacggataat ccgaataaaa
    10261 aaacattctg ggataaagtc catctcgatc ccacaatgct gctgatttta ctggcattgc
    10321 tggtttacag cgccctggtt atctggagcg ccagcggtca ggatattggc atgatggagc
    10381 gtaaaatcgg ccaaatcgcg atgggtctgg tcatcatggt ggtgatggcg caaattcctc
    10441 cacgcgttta tgaaggctgg gccccctatc tctatatcat ctgtattatt ttgctggtgg
    10501 cggtagatgc tttcggtgcc atctctaaag gcgctcaacg ctggctggac ctcggtattg
    10561 ttcgttttca gccgtcggaa attgccaaaa tagccgtacc actgatggtt gcgcgcttta
    10621 tcaaccgcga cgtttgcccg ccatcgttga agaacactgg catcgcgctg gtgctgatat
    10681 ttatgcccac gctgctggtg gctgcacagc ctgacctggg aacatcaatc ctcgttgcgc
    10741 tttccggtct gtttgtactg ttcctctctg gccttagctg gcgtctgatt ggcgtcgcag
    10801 tagtgctggt agcggcgttc attccgattc tgtggttctt cctgatgcat gattaccagc
    10861 gccagcgcgt aatgatgctc ctggacccgg aatcagaccc actcggcgcg ggctatcaca
    10921 ttattcagtc taaaattgct attggctccg gcggattacg cggcaaaggc tggctgcacg
    10981 gcactcagtc acagcttgaa tttctccccg aacgccatac tgactttatc ttcgcggtac
    11041 tggcggaaga gctgggatta gtgggcattt tgattctgct cgctctctac attctgctga
    11101 tcatgcgcgg gctgtggata gccgccagag cgcaaaccac ctttggtcgc gtcatggctg
    11161 gcggcttaat gctgatatta ttcgtttatg tcttcgtaaa tattggtatg gtaagcggta
    11221 ttctgccggt tgtaggggtt ccgctcccac tggtcagtta tggaggatcg gcgctaattg
    11281 tgctgatggc tgggttcggg attgtaatgt caatccacac ccacaggaaa atgttgtcga
    11341 aaagcgtgta agaggtgcgc aatgcgtaag cagtggctcg ggatctgcat cgcggcagga
    11401 atgctcgcgg catgtacaag cgatgatggt cagcaacaga cagtaagtgt accgcagcct
    11461 gcggtatgta acggccccat agttgaaatt agcggggcgg acccgcgttt cgaaccactg
    11521 aacgcgacgg caaatcagga ttaccagcgc gacggtaaaa gctacaaaat cgtgcaggat
    11581 ccgtctcgat ttatccaggc gggactggcg gcaatctatg atgccgaacc aggcagtaac
    11641 ctgacggcct ctggcgaagc tttcgatccg acacagctga cggcggccca tccaacgctt
    11701 ccgatcccca gctacgccag aatcactaac ctggctaacg ggcgaatgat cgtggtgcgc
    11761 attaatgatc gcggtcctta cggcaacgac cgcgttattt cgctttctcg cgcagcagct
    11821 gaccgtctta acacgtcaaa caacaccaaa gttcgtatcg atccgattat tgtcgcccag
    11881 gatggttcgc tttctggtcc tggtatggct tgtaccacag tcgccaaaca gacttacgcc
    11941 ctgcctgcac ctcccgattt aagcggtggc gcgggaacaa gttcagtgtc tggcccgcag
    12001 ggtgacattc ttccggtcag taattcgacg ctaaaaagcg aagatccgac cggcgcgccg
    12061 gtaaccagca gcggtttcct cggcgcacca acgaccttag cgccaggtgt actggaaggc
    12121 agcgaaccga cgcctgctcc acagcccgtt gttacagctc cgtcgacaac gcctgcaacc
    12181 tcgcctgcaa tggtgacacc gcaagccgcc tcgcaaagcg ccagcggcaa ctttatggtg
    12241 caggtcgggg ccgtaagcga tcaggctcgt gcgcaacagt accaacagca actgggacag
    12301 aagttcggcg tccccggtcg cgtaactcaa aatggcgcgg tctggcggat ccagcttggc
    12361 ccattcgcca acaaagccga agccagtacc ttgcagcaac gtttgcaaac cgaagcccaa
    12421 ttacagtcat ttattaccac cgcgcagtag cgtaaagcag gcatctgatg tgtcaatttc
    12481 tgtaaatgac gttaacaaag tcacgcggaa agtcagatgc ctgccggtag tgcatttgct
    12541 atagtagggc acttttttta attccatcac ggatgtcgta gttcagacca tgaataccat
    12601 tttttccgct cgtatcatga agcgcctggc gctcaccacg gctctttgca cagcctttat
    12661 ctctgctgca catgccgatg acctgaatat caaaactatg atcccgggtg taccgcagat
    12721 cgatgcggag tcctacatcc tgattgacta taactccggc aaagtgctcg ccgaacagaa
    12781 cgcagatgtc cgccgcgatc ctgccagcct gaccaaaatg atgaccagtt acgttatcgg
    12841 ccaggcaatg aaagccggta aatttaaaga aactgattta gtcactattg gcaacgacgc
    12901 atgggccacc ggtaacccgg tgtttaaagg ttcttcgctg atgttcctca aaccgggcat
    12961 gcaggttccg gtttctcagc tgatccgcgg tataaacctg caatcgggta acgatgcttg
    13021 tgtcgccatg gccgattttg ccgctggtag ccaggacgct tttgttggct tgatgaacag
    13081 ctacgttaac gccctgggcc tgaaaaatac ccacttccag acggtacatg gtctggatgc
    13141 tgatggtcag tacagctccg cgcgcgatat ggcgctgatc ggccaggcgt tgatccgtga
    13201 cgtaccgaat gaatactcga tctataaaga aaaagaattt acgtttaacg gtattcgcca
    13261 gctgaaccgt aacggcctgt tatgggataa cagcctgaat gtcgacggca tcaaaaccgg
    13321 acacactgac aaagcaggtt acaaccttgt tgcttctgcg actgaaggcc agatgcgctt
    13381 gatttctgcg gtgatgggcg gacgtacttt taaaggccgt gaagccgaaa gtaaaaaact
    13441 gctaacctgg ggcttccgtt tcttcgaaac cgttaaccca ctgaaagtag gtaaagagtt
    13501 cgcctctgaa ccggtttggt ttggtgattc tgatcgcgct tcgttagggg ttgataaaga
    13561 cgtgtacctg accattccgc gtggtcgcat gaaagatctg aaagccagct atgtgctgaa
    13621 cagcagtgaa ttgcatgcgc cgctgcaaaa gaatcaggtc gtcggaacta tcaacttcca
    13681 gcttgatggc aaaacgatcg aacaacgccc gctggttgtg ttgcaagaaa tcccggaagg
    13741 taacttcttc ggcaaaatca ttgattacat taaattaatg ttccatcact ggtttggtta
    13801 aaaattgaac acttgaaagt gtaatttccg tccccatata ctaagcatca gtaaaaaact
    13861 cccgccttct ggcgggagtt gctatttaat tacgttacgc cggagctgac atgaaaacca
    13921 aacttaacga actgcttgaa ttccctactc cttttactta caaagttatg gggcaggcgt
    13981 tacctgagct ggttgatcag gtggttgaag tggtacagcg ccatgcgcca ggtgactaca
    14041 ccccaacggt aaaaccaagc agcaaaggca actaccactc ggtatctatc actatcaacg
    14101 ccactcatat cgagcaggtt gaaacactgt atgaagaact gggcaaaatc gatattgtcc
    14161 gcatggttct gtaactcgct tctccgttac ccggcccctg gtcgggtaac tcccctctcc
    14221 tcgctgtgat atacttttct cccactttta ctcattctcc acggagatgc cgttttgtat
    14281 caggataaaa ttcttgtccg ccagctcggt cttcagcctt acgagccaat ctcccaggct
    14341 atgcatgaat tcaccgatac ccgcgatgat agtacccttg atgaaatctg gctggtcgag
    14401 cactatccgg tattcaccca agggcaggca ggaaaggcgg agcacatttt aatgcccggt
    14461 gatattccgg tgattcagag cgatcgcggt gggcaggtga cttatcacgg gccggggcaa
    14521 caggtgatgt atgtattgct taacctgaaa cgccgtaaac tcggtgtgcg tgaactggtg
    14581 accttgcttg agcaaacagt ggtgaatacc ctggctgaac tgggtataga agcgcatcct
    14641 cgggctgacg cgccaggtgt ctatgttggg gaaaagaaaa tttgctcact gggtttacgt
    14701 attcgacgcg gttgttcatt ccacggtctg gcattaaacg tcaatatgga tctttcacca
    14761 tttttacgta ttaatccttg tgggtatgcc ggaatggaaa tggctaaaat atcacaatgg
    14821 aaacccgaag cgacgactaa taatattgca ccacgtttac tggaaaatat tttagcgcta
    14881 ctaaacaatc ctgacttcga atatattacc gcttaatttc atacctcaat ggcccaattt
    14941 acactgggtc attactcata acacctgcta atgctttaca tctccttcgc gtttgtatat
    15001 tttctttatc acttgcagaa atgcgatgct acgccttgct ccgtaactca ccccattgat
    15061 taacttattg aaatagttga ttatgatgag
//
LOCUS       DAZKIP010000104        15062 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZKIP010000104 DAZKIP010000000
VERSION     DAZKIP010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15062
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.105"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(115..2394)
                     /gene="maeB"
                     /locus_tag="ACHOUK_003535"
     CDS             complement(115..2394)
                     /gene="maeB"
                     /locus_tag="ACHOUK_003535"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFP9898909.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2683..3633
                     /gene="tal"
                     /locus_tag="ACHOUK_003536"
     CDS             2683..3633
                     /gene="tal"
                     /locus_tag="ACHOUK_003536"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFP9898910.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3653..5656
                     /gene="tkt"
                     /locus_tag="ACHOUK_003537"
     CDS             3653..5656
                     /gene="tkt"
                     /locus_tag="ACHOUK_003537"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFP9898911.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKDIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5751..6794)
                     /gene="ypfG"
                     /locus_tag="ACHOUK_003538"
     CDS             complement(5751..6794)
                     /gene="ypfG"
                     /locus_tag="ACHOUK_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFP9898912.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSDLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVVVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(6920..7495)
                     /gene="nudK"
                     /locus_tag="ACHOUK_003539"
     CDS             complement(6920..7495)
                     /gene="nudK"
                     /locus_tag="ACHOUK_003539"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFP9898913.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLID"
     gene            complement(7563..9542)
                     /gene="aegA"
                     /locus_tag="ACHOUK_003540"
     CDS             complement(7563..9542)
                     /gene="aegA"
                     /locus_tag="ACHOUK_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFP9898914.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHLTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLIRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9748..11448
                     /gene="narQ"
                     /locus_tag="ACHOUK_003541"
     CDS             9748..11448
                     /gene="narQ"
                     /locus_tag="ACHOUK_003541"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFP9898915.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGTPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLGRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYRSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            11612..14725
                     /gene="acrD"
                     /locus_tag="ACHOUK_003542"
     CDS             11612..14725
                     /gene="acrD"
                     /locus_tag="ACHOUK_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFP9898916.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATAEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(14824..14883)
                     /gene="ypfM"
                     /locus_tag="ACHOUK_003543"
     CDS             complement(14824..14883)
                     /gene="ypfM"
                     /locus_tag="ACHOUK_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFP9898917.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         3528 a         3991 c         3926 g         3617 t
ORIGIN      
        1 ttttatatac tgcttttttt attattcgca aactcagtaa taagctttac taatatattg
       61 cagctaaacc gcttaccctg aatattcagg gtaagcgtga gagttaaaaa agaattacag
      121 cggttgggtt tgcgcttcaa ccacggccag cgccaccatg ttgacgatac gacgcaccga
      181 tgcgatcggc gttaacacgt gaaccggttt cgccacgccc atcagcaccg gaccgacagt
      241 cacaccttcc gagctggaaa cgcgcagtaa gttgtaacta atgcgggcag cttccatgtt
      301 cggcatcacc agaatattgg cagaaccttt caaagggctg tccggcatac ggtcgttgcg
      361 aatcgcttcc accagcgctg catcgccgtg catttcacca tcaatcatca gttctggcgc
      421 acgttccctg accagttcca gcgcctgacg cattttgctc gacgacgggc agtcagaaga
      481 accaaagttg gagtgcgaca acagagcaac gcgcggttca ataccgaaac gacggacagt
      541 ttctgccgcc atcaaggtga tctccgccag ctcttctgcg tctggttcgt cattgacgta
      601 ggtatcggca ataaaggtgt taccactcgg cagcagcagc gcgttcattg cacctgcggt
      661 gtgaacgcca tcgcgataac caaagacatt tttcaccacg ctaaaatgtt catgataatc
      721 acccaccgta ccgcaaatca ttgcatcggc ttcgccacgc tgaaccatga tcgcgccgat
      781 cactgtcggg ttactgatca gcgcccgctg cgcctgttcc tgagtgacgc cgcgacgctt
      841 catgatctgg aagtattcgg tccagtactc tttaaagcgc ggatcggatt cgttattgac
      901 gatctcaaaa tcaacgcccg ctttgatctg caagcccagt ttctgaatgc gcatttcgat
      961 cacgttcgga cgaccgataa ggatcggttt cgccagtccc agcgttacca gttcctgagt
     1021 ggcatgcaga acgcgcgcct cttccccttc cggcagaact acgcgcttcg gcgctttgcg
     1081 agcctgggag aaaatcggct tcataaacag gttggttttg taaacgaact cagtcagctt
     1141 gtcgatgtag acgtcgaaat cagcaatcgg acgcgtcgcc acgcccgact ccatcgccgc
     1201 tttcgcgacc gcaggagcaa tcttgactat cagacgcgga tcaaacggtt ttggaatgat
     1261 gtattcaggg ccaaagctca gatcctgatc gccatacgct gaagccacca cttcactctg
     1321 ttccgcatgg gccagttctg caatcgcgcg taccgccgcc agtttcatct cttcgttgat
     1381 ggcggttgcg ccaacgtcca gcgcgccacg gaagatgaac gggaagcaaa ggacgttgtt
     1441 cacctggttc gggtagtcgg aacggccggt acagatgatg gcatccggac gcacttcttt
     1501 cgccagcggc ggcagaattt ccggttccgg gttcgccagc gccaggatca ttggcgctcg
     1561 agccattttc ttcaccattt cctgggtcag cactttcggg ccggaacagc cgaggaaaat
     1621 atctgcgcct tcaatcacat catcgagggt acgtttgccg tcatccacta ccgcatatgc
     1681 ggctttggtt tccgccatgt ttggctcacg gccctgatag ataacgcctt ttgaatcgca
     1741 aaccacgatg ttatgttttt gcagacccaa cgctaccagc aggttcatac aggcgattgc
     1801 tgcagcaccc gcgccggaaa ccaccatacg cacgtcagag atgtttttct ccaccacgcg
     1861 caagccgttg aggatggcgg cagtgctgat aattgccgtg ccgtgctgat cgtcgtggaa
     1921 taccggaata ttcatccgct cgcgcagttt ctgttcaata tagaaacatt ctggcgcttt
     1981 aatgtcttcg aggttgatgc cgccgaaggt cggttcgagc gcagcgacga cttcaataaa
     2041 tttgtccggg tcgagttcat caacttcaat gtcaaataca tcaatcccag cgaatttctt
     2101 aaacagaacg cccttgcctt ccatcaccgg tttgcctgcc agcgcgccaa tgttgcctaa
     2161 ccccagcacc gccgtaccgt tagagatcac cgccaccagg ttaccgcgcg cggtatattt
     2221 gtaggctttt aacgggtctt tttcgatttc aagacaaggt gcggcaacgc ctggtgagta
     2281 ggccagcgcc agatcgcgct gcgttgccag aggcttggtt ggagaaacct ggattttccc
     2341 tggaactgga aattcatgga aatcaagtgc actttgtttt aactggtcat ccatttggtt
     2401 gttcctttca cgtaacgttc acaaataaag tgtgtgggca acagcccctg cccacaacgt
     2461 ggcgcacatt attaccttgc cggagtctac agactttgag caagtccaaa ctctcaccat
     2521 taatataatg ttttggtaat aatcctataa cactgatgtt acctgcttaa tccagcaata
     2581 ccatgcctgt ctgctatgct tttttgatgc gtttagcgaa atttctcaga agtgtgaatt
     2641 aacgcactca tctaacactt tacttttcaa ggagtatttc ctatgaacga gttagacggc
     2701 atcaaacagt tcaccactgt cgtggcagac agcggcgata ttgagtccat tcgccattat
     2761 catccccagg atgccaccac caatccttcg ctgttactca aggctgccgg attatcacaa
     2821 tatgagcatt taatagacga tgctatcgcc tggggtaaaa aaaatggcaa gacccaggaa
     2881 caacaggtgg tcgcagcgtg tgacaaactg gcggtcaatt tcggtgctga aatcctcaaa
     2941 atcgtacccg gtcgcgtgtc aacagaagtt gatgcacgcc tctcttttga taaagaaaag
     3001 agtattgaga aggcgcgcca tctggtggac ttgtatcagc aacaaggcgt tgagaaatca
     3061 cgcattctga tcaaactggc ttcgacctgg gaaggaattc gcgcggcaga agagctggaa
     3121 aaagaaggta ttaactgcaa cctgacgctg ctgttttctt ttgcacaggc gcgggcctgt
     3181 gcagaagcgg gcgtttttct gatttcgccg tttgtcgggc gtatttatga ctggtatcag
     3241 gcacgcaagc cgatggaccc atatgtggtg gaagaagatc cgggcgttaa atcggtgcgc
     3301 aatatctacg actactataa gcaacaccac tatgaaacca ttgtgatggg cgcgagcttc
     3361 cgtcgcaccg aacaaatcct cgccttaacc ggctgcgatc gactgactat cgcaccgaat
     3421 ttgctgaagg agctgcagga aaaagtttcg ccagtggtac gtaaattaat cccaccttct
     3481 cagacgttcc cacgcccagc tcccatgagc gaagcggagt tccgttggga gcacaatcag
     3541 gatgcgatgg cggtagaaaa actgtctgaa ggcattcgtc tgttcgccgt tgatcaacgc
     3601 aaactggaag atcttcttgc cgccaaacta taaaccagcc acggagtgtt atatgtcccg
     3661 aaaagacctt gccaatgcga ttcgcgcact cagtatggat gcggtacaaa aagccaattc
     3721 tggtcatccc ggcgcgccga tgggcatggc tgatattgcc gaagtgctgt ggaacgattt
     3781 tcttaaacat aaccctaccg acccaacctg gtatgatcgc gaccgcttta ttctttccaa
     3841 cggtcacgcg tcgatgctgc tctacagttt gctgcatctg accggttacg acctgccgct
     3901 ggaagagctg aagaactttc gtcaactgca ttcgaaaacc cctggccacc cggaaatcgg
     3961 ctatacgccc ggagttgaaa ccaccaccgg tcctcttgga caaggtttgg cgaacgccgt
     4021 cgggctggcg atagcggaac gtacactggc ggcgcagttt aaccagccgg atcatgaaat
     4081 tgtcgatcac ttcacctatg tgtttatggg cgacggctgc ctgatggaag gtatttccca
     4141 cgaagtctgt tcgctggcgg gcacgctggg actgggcaag ctgattggtt tttacgatca
     4201 caacggtatt tcgattgatg gagaaaccga aggctggttt accgacgata cggcaaaacg
     4261 ttttgaagcc tatcactggc atgtgatcca tgaaatcgac ggtcacgatc cgcaggcggt
     4321 gaaggaagca atccttgaag cgcaaagcgt gaaagataag ccgtcgctga ttatctgccg
     4381 tacggtgatt ggctttggtt cgccgaataa agcaggtaag gaagaggcgc acggcgcacc
     4441 gctgggggaa gaagaagtgg cgctggcacg gcaaaaactg ggctggcacc atccgccatt
     4501 tgagatccct aaagatattt atcacgcttg ggatgcccgc gaaaaaggcg aaaaagcgca
     4561 gcagagctgg aatgagaagt ttgccgccta taaaaaggct catccgcaac tggcagaaga
     4621 gtttacccgt cggatgagcg gtggtttacc gaaggactgg gagaaaacga ctcagaaata
     4681 tatcaatgag ttgcaggcga atccggcgaa aatcgctacc cgtaaggctt cgcaaaatac
     4741 gcttaacgct tacgggccga tgctaccgga gctgctcggc ggttcggcgg atctggctcc
     4801 cagcaacctg accatctgga aaggttctgt ttcgctgaag gaagatccgg cgggcaacta
     4861 cattcactac ggggtgcgtg aatttggcat gaccgctatc gccaacggca ttgcgcacca
     4921 cggcggcttt gtgccgtata ccgcaacgtt cctgatgttt gttgaatacg cccgtaacgc
     4981 cgcgcggatg gcggcactga tgaaagcgcg gcagattatg gtttataccc acgactcaat
     5041 tggtctgggc gaagatggtc cgacgcacca ggctgttgag caactggcca gcctgcgctt
     5101 aacgccaaat ttcagcacct ggcgaccgtg cgatcaggtg gaagcggcgg tgggctggaa
     5161 gctggcggtt gagcgccaca acggaccgac ggcactgatt ctctccaggc agaatctggc
     5221 ccaggtggaa cgtacgccgg atcaggttaa agagattgct cgtggcggtt atgtgctgaa
     5281 agacagcggc ggtaagccag atattatttt gattgccacc ggttcagaga tggaaatcac
     5341 cctgcaagcg gcggagaaat tagcgggaga aggtcgcaat gttcgcgtgg tttccctgcc
     5401 ctcgaccgat attttcgacg cccaggatga ggaatatcgg gagtcggtgt tgccttctaa
     5461 cgttgcggct cgcgtggcgg tggaagcagg tattgccgat tactggtaca agtatgttgg
     5521 tctgaaaggg gcaattgtcg ggatgacggg ttatggggaa tctgctccgg cggataagct
     5581 gttcccgttc tttggcttta ccgccgagaa tattgtggca aaagcgcata aggtgctggg
     5641 agtaaaaggt gcctgatggt gatcgccgga tgctgattgc cggatgcgac gctgacgcgt
     5701 cttatccggc ctacagcgtc ttatccggcc tacatgtccc gccattttgt ttaacgggtg
     5761 atccacaacg tgggccaggc gtctggccca tgccagttat cgcaggtggg ttctgcggcg
     5821 taacgcacca ggcgaaaacg ctgaccgtca aagcgccagc gcgcctgaat gccacaatcg
     5881 cttaatccgc gccctttcgc taaggtcacc agttcacgcg atttctcatc aaatgttgcg
     5941 ttcatcagtt ccagttcatt cgtctcctga ccgttgttga acggcaaacg cagccggacc
     6001 gggcgcgagg ccagcggctt tttacgcgac acaatccatg ccaaatcaat ggtgttatag
     6061 gcccctgcct cacagctaat catcatcagc gctttatcat cagtcagcgc agtgacattc
     6121 acctcacgac gcaatggatc aagcgagcag cgcagaccat tcatccgcca gtttccataa
     6181 tccagcaaat cgttgcgttc ttcgagtgag agtggcgtcg gcgttgggtt aaccaccacg
     6241 acctctttca gcgcaggcgc gggcggtacg ctgagcggcg gttcgtcccc tttcttgatc
     6301 cacgcggttt cactgccaac gcgcttttgc tgagcatcaa taaacaacaa cgccgctttt
     6361 aagtcactca gagaaatggt ctgattgccg tcgcgtaagg tgatcgcctt cccttcctga
     6421 atcatctgca aaaacgcggt gatggttgcc gtatcatcgg ttactaataa ccatggtgaa
     6481 atccgccact tgtcaccact taacgccaac ggctcgccat ctaacagcag ccgtggcgca
     6541 atttcccctt cagacgcatc cggcgacttc aacccgccgc gctcaatacg taaaacggca
     6601 tcggtatgcg ccccggcgct gcggctcagg gtcatcacca gtccattatg atcgcccgta
     6661 ttacgcgcca cgcagaagtt ttggttatta caggtgacct gccagtcgga aaacgcccgt
     6721 tgcgctggtg ccgcccacac caaagacgtc ggcaacaaag cgaaaaaaaa gagaaggaaa
     6781 atgcgatagc gcatggacgg tacgacccca gaagttcaaa caaacagcac aagtcgtatc
     6841 ttcgttgcgc aggtagcgcg gctcaatcgg atttgtcgga ttgagccaag aataaacaac
     6901 gaaatattat tgttattttt cagtctatta aatgtgacat ttgcaaatag ttaagcaata
     6961 acaccgtctt accgtcacgt atctcgccgg ttttgatcat ctccagcgcc tggctgaacg
     7021 gcagctcgag cacttcaata tcttcatctt cgacaccgcc accggcgtta gcgcgctgat
     7081 tgtcactgta ttcggcgata aaaaagtgga ttagctcagt cacaccgcct ggcgacatat
     7141 acagttcaaa taatttgcgc acttcgccca cttcatagcc cgtctcttca atcgcttctt
     7201 tgcgaatgca cacttccggt tcgtcgttat ccagcagccc ggcgcaggtt tcaatcagct
     7261 gcccgctttc attgccatta acccaggtag cgacacggaa ctgacgaatc agaaccacgg
     7321 tctttttctt cgcgttgtac aggaggatcg tcgcaccatt accgcgatcg taaacttcac
     7381 gtttatggcg gataacttcg ccatctttgc gcgtgagatc gtaagtaatg ttgtgcaggg
     7441 tgaaatagtt atcggagaga attttgtctt taatgagggt gatttgttgc gtcataccga
     7501 ctccacagcg cgaaatgaac aattatatta cgctgtgaag tcgggtttgt ctgcgcaggc
     7561 tatcagtgag atttgactga ttttaccccc agccagtcaa taatcccctg tgccgcatga
     7621 cgtccttctg ccattgcggt aaccaccaga tccgcaccac gcacggcgtc gccaccggcg
     7681 aagattttcg gattggtggt ctggtaacgg tactggcttt ccacatccgc gatgatgcgg
     7741 ccccatttgt ctaccgttac accgtgcgac tccagccacg gcatcccgtg cggattgaag
     7801 ccaaacgcca taatcaccgc gtcggctggc atgacaaatt cactgccttc caccggcact
     7861 ggacgccgac gcccctgggc atccggctcg ccaagacgcg tgcgcaggaa acgaatcccg
     7921 cagacgtgac cttgttcatt cagctcaagc gccaccggct ggacgttaaa ttcgaagttg
     7981 gccccctctt cgcgggcgtt cttcacctct ttcttcgagc ctggcatgtt agcttcatca
     8041 cgacgataag cgcaggtgac gttactcgcg ccgtggcgca gcgcagtacg cacacagtcc
     8101 atcgcggtgt cgccgccgcc cagtaccacg acgttaagtc cggcggtatt gataaacggc
     8161 tcttccggta gctcttcgag ccccatcacc tgtttagtgt tggcgatgag gaacggcagc
     8221 gcgtcataaa cgcccggcgc atcttcattg ggtaaacccg ctttcatgga acggtaagtg
     8281 cctacgccaa cgaagaccgc gtcgtattgt tccaaaagcg aatccaaaga gacatcttta
     8341 cccacttcac aattgagttc gaagtgaatc cccatcgcgc tgaagatttc ccgacgacgt
     8401 gccagcaggg atttatccag tttgaaagaa ggaatgccga aagtgagcaa gccaccgatt
     8461 tctggatggc gatcgtacac cgtcaccgcc acgccattgc ggatcagaac atccgcacag
     8521 gccagccctg ccggacctgc accgataatc gccacccgct tgtccacttt ggtgacatgg
     8581 cttaagtcag gacgccaacc tttcgccaac gcctgatctg aaatgtagcg ttcaatgttg
     8641 ccgatagtta ccgcaccgtg ctcatcgcga atagtacagg caccttcaca caaacggtct
     8701 tgcggacaaa cgcgtccggt aatttccggc agggtgttgg tcaggtgaga aagctcgacg
     8761 gcggcgtcga tgtttccggc tttcaccagt tcaatccact gcggtatatg gttatgcagc
     8821 gggcaggtcc attcacaaac gctgtgctcg ccgcatttaa ggcagcgcga ggcttcccgt
     8881 tgtgcctgat cggcacggaa tggcagataa atttcatcaa aaccggtttt gcgcgcttca
     8941 atcgccagtt tatccggctc gccacgcgcg ggcgttgcct gcatttgttc gactttactc
     9001 attaccggca tttcttgtgc agcggtactg gcatgccacg gttgatgttc ctgacgtgcg
     9061 gtacgcaagc ggcgggattt cgccatgcct gagagtgcgg cgtcagtgac cagctgcagc
     9121 gcgtccgccg ggcaattctc gacacaggca ggaccgtttt cgcgccctgc acaaaggtcg
     9181 catttatgcg ccgtggcttt aactttgccc gctgcgacgg gtgtcaggac gatttgcatc
     9241 gtaccaaacg gacaggccac cacgcaggat ttacagccaa tacacttttg ctgattgacc
     9301 tgaatgctgt catcaacgtg gctgattgcg ccattagggc agctacgggc gcagggcgca
     9361 tcttcacaat ggtgacaggt cactgcacta cgttgctgct gatgtttgat aaccgtaatt
     9421 cggggatgaa aatggtgttg gctcaggaca tgttgctcat cattgtgagc catgacacaa
     9481 gcgatttcac aagcatgaca acccagacat tgctgactgt tggccataat aaaacgattc
     9541 ataacgacct tcttttttgg ttgtaaaaac cttattcttt atatgagtgt tgttattacc
     9601 cgacttacag gggaatcggc aatgttcatg tgcccagaat aagtaactat ttcgctataa
     9661 actgtggcag atcaaataat cccatcacta actaaattgc gtttcagcga actggaacat
     9721 taatgatttt ttgtggagaa gacgcgtgtg attgttaaac gacccgtctc ggccagtctg
     9781 gcccgggcct ttttttacat tgtgctgctg tcgattcttt ccacgggtat cgctctgcta
     9841 actctggcga gcagtttgcg cgacgctgag gctatcaata ttgccggatc gctgcgtatg
     9901 cagagttacc gcctgggcta cgacttgcaa agtggcactc cacaactcaa tgcacatcgc
     9961 cagctgtttc agcaggcact gcattcaccg gtattaacca acctcaacgt ctggtatgtg
    10021 ccagaagcag taaaaactcg ctatgcgcat ctgaatgcca actggctgga gatgaataat
    10081 cggctcagca agggcgattt gccgtggtat caggccaata ttaataatta tgttaatcag
    10141 atagacctgt tcgtgctggc tttacagcac tacgctgaac gcaaaatgct gctggtggtg
    10201 gcgatttccc tggctggcgg tatcggtatt ttcacgctgg tcttttttac tttgggccgc
    10261 atacgccatc aggtggttgc cccgctgaat cagctggtta ccgccagtca gcgtattgaa
    10321 cacggacagt tcgactcgcc accgctggat accagcctgc cgaatgagct tggtctactt
    10381 gcaaaaacct ttaaccagat gtcgagcgag ctgcataaat tgtaccgttc gctggaggcg
    10441 tcagtagaag aaaagacccg cgatctccac gaggccaagc gtcgtctgga ggtgttgtat
    10501 caatgttcgc aggcgctgaa caccagccag attgatgtgc attgtttccg ccatattttg
    10561 cagattgttc gcgacaatga agcggctgaa tatctggagt taaatgtcgg tgaaaactgg
    10621 cggattagcg aagggcaacc aaacccggaa ttgccgatgc agattttacc ggtgacaatg
    10681 caagagacgg tttacggcga actgcactgg caaaatagtc acgtttcatc atcagaaccg
    10741 ctgcttaaca gcgtttcgtc gatgctggga cgcggtttgt actttaatca ggcgcagaag
    10801 cattttcagc agttattgtt gatggaagaa cgtgcaacca tcgcccgcga attgcacgac
    10861 tcgctggctc aggtactttc ttacttacgt atccagttga cgttacttaa gcgttcgata
    10921 ccggaagata acgccaccgc acaaagtatc atggccgatt tttcccaggc gttgaatgat
    10981 gcttatcggc agttacgcga gctgttgact acttttcgcc tgacgctgca gcaggcggat
    11041 ctcccctccg cgttgaggga aatgctggat acgttacaaa atcaaaccag cgccaaactg
    11101 accctcgact gccgtctgcc aaccctggca ctggatgcgc aaatgcaggt gcatttgttg
    11161 caaattattc gcgaagcggt gctgaatgcg atgaagcacg ccaacgccag cgaaatcgcc
    11221 gtcagttgcg tcaccgcgcc ggacggcaat cacacggttt atatccgtga taacgggatt
    11281 ggtatcggtg aaccgaaaga acccgaaggt cattatggtc tgaatatcat gcgcgaacgc
    11341 gcggaacggc taggtgggac gctgactttt tcacaacctt ccggcggcgg cacgttagtg
    11401 agtattagct ttcgctctgc ggagggtgag gaaagtcagt tgatgtaatg cctcctactg
    11461 accaaagaat acttgcagtt aaggttcagt ataaaagggc atgataattt acattaactc
    11521 ctttttttct ccacgattgg ctcgtacctt gccgctacag tgaagcaagt caagcctaca
    11581 acgatacgca gaaacacgag gtcctctttt aatggcgaat ttctttattg atcgccccat
    11641 ttttgcctgg gtgctggcaa tcctgttgtg tctgacaggt accctggcga ttttttcatt
    11701 gcccgttgaa caataccccg atctcgcgcc accgaatgtg cgagtgaccg ctaactatcc
    11761 cggcgcatcg gcccagacgc tggaaaacac cgtgacccag gttatcgagc aaaatatgac
    11821 cggcctcgat aatctcatgt atatgtcatc tcagagcagt ggcaccggtc aggcatctgt
    11881 cactttaagt tttaaagcag gcaccgatcc ggacgaagcc gtgcagcaag tacaaaacca
    11941 gctgcaatca gccatgcgaa agttaccgca ggcggtgcaa aatcagggcg tgactgtgcg
    12001 taaaaccggc gataccaaca ttctgaccat tgccttcgtc tctaccgatg gttcgatgga
    12061 taaacaggat attgctgatt atgttgccag taatattcag gacccgttaa gccgcgtaaa
    12121 tggcgtcggg gatatcgatg cctatggttc gcaatattcc atgcgtatct ggctggaccc
    12181 ggcgaaactc aacagtttcc agatgacagc taaagatgtc actgatgcca ttgagtcaca
    12241 gaacgcgcag attgcggttg ggcaacttgg tggtacacct tccgtcgata agcaggcgct
    12301 caacgccacc attaacgccc agtcactgct gcaaacacca gaacagttcc gcgatatcac
    12361 cttgcgggtg aatcaggacg gctcagaagt aaggctcggc gatgtcgcca ccgccgaaat
    12421 gggggcggag aaatacgatt atcttagccg cttcaatggt aagccagcct ccgggttggg
    12481 ggtaaaactg gcctccggcg cgaacgaaat ggcgacagcg gagctggtgc tcaatcgtct
    12541 cgacgagctg gcgcagtatt tcccgcacgg actggaatac aaggtggcgt atgaaaccac
    12601 ctcgtttgtt aaagcctcca ttgaagacgt ggtgaaaacg ctgctggaag ctatcgctct
    12661 ggttttcctc gttatgtatc tgttcctgca aaacttccgc gccacgctga tacccactat
    12721 cgccgtgccg gtggtgttga tgggaacctt ctccgtactt tacgccttcg gttacagcgt
    12781 caacacctta accatgttcg cgatggtgct ggcgatcggt ctgctggtgg atgacgccat
    12841 cgtggtggtg gaaaacgtcg aacgtattat gagtgaggaa ggactcactc ctcgcgaagc
    12901 cacgcgtaaa tcgatggggc agatccaggg ggcactggtc gggattgcga tggtgctttc
    12961 ggcggtattt gtaccaatgg ccttcttcgg cggcaccacc ggtgccatct atcgccagtt
    13021 ctctattacc attgttgcgg cgatggtgct gtcagtactg gtagcgatga tcctcactcc
    13081 ggctctgtgt gccacactac ttaagccact gaaaaaaggt gagcatcacg ggcaaaaagg
    13141 cttttttgcc tggtttaacc agatgtttaa ccgcaacgcc gaacgctacg aaaaaggggt
    13201 ggcgaaaatt ctccaccgta gcctgcgctg gattgtgatt tatgtcctgc tgcttggcgg
    13261 catggtgttc ctgttcctgc gtttgccgac gtcgttctta ccgctggaag accgtggcat
    13321 gtttactacc tcggtacaat tgcccagcgg ttcaacccaa caacagaccc tgaaagtcgt
    13381 tgagcaaatc gagaaatatt acttcaccca tgaaaaagac aacatcatgt cggtgtttgc
    13441 cactgttggt tctggccctg ggggtaacgg gcaaaacgtg gcgcgaatgt ttatccgcct
    13501 gaaagactgg agcgaacgcg acagtaagac cggcacctcg tttgccatta tcgagcgtgc
    13561 aacaaaggcg tttaaccaaa ttaaagaagc tcgcgttatc gccagcagcc cgccagcaat
    13621 tagcggtctt ggtagttctg caggttttga tatggagttg caggaccacg ctggagcagg
    13681 tcacgatgcg ctgatggcag cacgtaacca gttgctggcg ctggcggcgg aaaacccgga
    13741 gctaacccgt gtgcgccata acggcctcga tgacagtccg cagttgcaga ttgatatcga
    13801 ccagcgtaaa gctcaggcgc tgggcgttgc tatcgacgat attaacgaca cactgcaaac
    13861 cgcctggggt tcgagctatg tgaatgactt tatggatcgc ggtcgcgtga agaaagtcta
    13921 tgtgcaggca gctgcgccgt atcgcatgct gccagatgac atcaatctct ggtatgtccg
    13981 aaataaagat ggcggcatgg tgcccttctc tgctttcgcg acctcacgct gggagacagg
    14041 ctcgccgcgt ctggaacgct ataacggtta ttctgcggtt gaaattgttg gggaagccgc
    14101 accgggggtc agtaccggta cggcgatgga tattatggaa tcgttagtga agcagctacc
    14161 aaacggcttt ggtctggagt ggacggcgat gtcgtatcag gagcggcttt ccggcgcgca
    14221 ggctccggcg ctgtacgcca tttccttgct ggtggtattc ctgtgtctgg ctgcattgta
    14281 tgaaagctgg tcggtaccgt tctcggtaat gctggtcgtg ccgctggggg taatcggcgc
    14341 gctgctggca acctggatgc gcgggctgga aaacgacgtt tacttccagg tgggcctgtt
    14401 aacggtcatt ggtttatcgg cgaaaaacgc catcctgatc gtcgagtttg ctaacgagat
    14461 gaaccaaaaa ggccacgacc tgtttgaagc gacgctccac gcctgccgtc agcgtttacg
    14521 cccgattctg atgacctcgc tggcatttat cttcggcgta ttgccaatgg caaccagcac
    14581 gggtgccggt tccggtggtc agcatgcggt gggtactggc gtaatgggcg ggatgatttc
    14641 agccactatt ctggctattt acttcgtgcc gctgttcttt gtgctggtgc gccgccgctt
    14701 cccgctgaag ccgcgcccgg aataagcaat aaaaaaggcg acatgccaat gtgtcgcctt
    14761 tttcaacttt ccgattaaga acctgctcag cgggttcttg ctgtttgtac tttgtctcag
    14821 gaattactta cgaagcataa cttcaataaa gtctttccag ttccccagtt cacgttcaat
    14881 cataacaacc tctcttataa ttatgggtat tctacggaaa caatataccg tggtgaagct
    14941 aatttactcg attgctgcga tgactacctc cgggggacaa accttatgta aatactatgg
    15001 tcctgcagtg atgatttgta tgtgatacac agcaacattt cgagatattc atacggcatc
    15061 ta
//
LOCUS       DAZKIP010000105        14712 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZKIP010000105 DAZKIP010000000
VERSION     DAZKIP010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.106"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            108..311
                     /gene="aaeX"
                     /locus_tag="ACHOUK_003544"
     CDS             108..311
                     /gene="aaeX"
                     /locus_tag="ACHOUK_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFP9898918.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVCRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            319..1248
                     /gene="aaeA"
                     /locus_tag="ACHOUK_003545"
     CDS             319..1248
                     /gene="aaeA"
                     /locus_tag="ACHOUK_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFP9898919.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQE
                     KRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAPA
                     DGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGSN
                     KVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWPA
                     GTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            1254..3221
                     /gene="aaeB"
                     /locus_tag="ACHOUK_003546"
     CDS             1254..3221
                     /gene="aaeB"
                     /locus_tag="ACHOUK_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFP9898920.1"
                     /translation="MDIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LCIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            3313..3585
                     /gene="yhcO"
                     /locus_tag="ACHOUK_003547"
     CDS             3313..3585
                     /gene="yhcO"
                     /locus_tag="ACHOUK_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFP9898921.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(3641..3904)
                     /gene="yhcN"
                     /locus_tag="ACHOUK_003548"
     CDS             complement(3641..3904)
                     /gene="yhcN"
                     /locus_tag="ACHOUK_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFP9898922.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(4269..4739)
                     /gene="argR"
                     /locus_tag="ACHOUK_003549"
     CDS             complement(4269..4739)
                     /gene="argR"
                     /locus_tag="ACHOUK_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFP9898923.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            5174..6112
                     /gene="mdh"
                     /locus_tag="ACHOUK_003550"
     CDS             5174..6112
                     /gene="mdh"
                     /locus_tag="ACHOUK_003550"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFP9898924.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            complement(6176..7243)
                     /gene="degS"
                     /locus_tag="ACHOUK_003551"
     CDS             complement(6176..7243)
                     /gene="degS"
                     /locus_tag="ACHOUK_003551"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFP9898925.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(7333..8700)
                     /gene="degQ"
                     /locus_tag="ACHOUK_003552"
     CDS             complement(7333..8700)
                     /gene="degQ"
                     /locus_tag="ACHOUK_003552"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFP9898926.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKTGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            complement(8854..9252)
                     /gene="zapG"
                     /locus_tag="ACHOUK_003553"
     CDS             complement(8854..9252)
                     /gene="zapG"
                     /locus_tag="ACHOUK_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFP9898927.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            9446..10573
                     /gene="zapE"
                     /locus_tag="ACHOUK_003554"
     CDS             9446..10573
                     /gene="zapE"
                     /locus_tag="ACHOUK_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFP9898928.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLVLAGGKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            10792..11220
                     /gene="rplM"
                     /locus_tag="ACHOUK_003555"
     CDS             10792..11220
                     /gene="rplM"
                     /locus_tag="ACHOUK_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFP9898929.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            11236..11628
                     /gene="rpsI"
                     /locus_tag="ACHOUK_003556"
     CDS             11236..11628
                     /gene="rpsI"
                     /locus_tag="ACHOUK_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFP9898930.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            12023..12661
                     /gene="sspA"
                     /locus_tag="ACHOUK_003557"
     CDS             12023..12661
                     /gene="sspA"
                     /locus_tag="ACHOUK_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFP9898931.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            12667..13164
                     /gene="sspB"
                     /locus_tag="ACHOUK_003558"
     CDS             12667..13164
                     /gene="sspB"
                     /locus_tag="ACHOUK_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFP9898932.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPSRGGRPA
                     LRVVK"
     gene            complement(13207..14563)
                     /gene="dcuC"
                     /locus_tag="ACHOUK_003559"
                     /pseudo
     CDS             complement(13207..14563)
                     /gene="dcuC"
                     /locus_tag="ACHOUK_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
BASE COUNT         3611 a         3623 c         3774 g         3704 t
ORIGIN      
        1 tatcaatcta cagcaatgtt gctctcgccc ggctttccat gcccttctct gcggcgacag
       61 atgctgaaaa taataacgcc tgctctctct atacaaccaa ggtcaacatg agtctgtttc
      121 ccgttatcgt ggtgtttggg ctgtccttcc caccgatatt ttttgaattg cttttatcac
      181 tggcgatttt ctggctggtg tgccgggtac ttgtgccaac aggtatctac gactttgtct
      241 ggcatccggc gttgttcaac accgcgctct attgctgctt gttttatttg atatcgcgac
      301 tgttcgtttg aggttgaagt gaaaacacta ataagaaaat tctcccgtac ggccatcacg
      361 gtcgtattag tcattctggc cttcatcgca atttttaatg cctgggtcta ttacaccgaa
      421 tccccctgga cgcgtgacgc gcgctttagc gctgacgtcg ttgcgatcgc gccggacgtt
      481 tctggactca ttacccaggt gaatgttcat aaccagctgg tgaaaaaagg acaggttctg
      541 ttcaccatcg accagccgcg ctatcaaaag gcgcttgagg aagcgcaagc cgatgttgct
      601 tattatcagg tactggcaca ggagaaacgc caggaggccg gacgtcgtaa ccgtctcggt
      661 gtgcaggcga tgtctcgcga agagatcgac caggccaaca acgtactaca aacggttctg
      721 catcagttag cgaaagcgca ggcgacccgc gatctggcaa aactggatct tgaacgcacg
      781 gtgatccgcg cgccagcaga tggctgggtg accaacctca acgtctatac cggtgagttt
      841 attactcgtg gatcaacggc ggttgcgctg gtgaaacaga actctttcta tgtactggcc
      901 tatatggaag aaactaagct ggaaggggtg cgccctgggt atcgtgcaga gatcacgccg
      961 cttggcagta acaaagtgct gaaagggact gttgatagtg ttgccgctgg ggtcaccaac
     1021 gccagcagca cgcgtgacga caaagggatg gcgaccatcg actctaacct tgaatgggtg
     1081 cgtttggcgc aacgtgttcc ggttcgtatt cgtctcgaca accagcaaga gaacatctgg
     1141 cctgcgggca ccactgctac agtggtggtc actggcaaac aagatcgcga cgaaagccag
     1201 gattcgttct tccgtaaaat ggcccatcgc ctgcgtgagt ttggttaatc acgatggata
     1261 ttttctccat tgctaaccaa catattcgct ttgcggtaaa actggcgacc gccattgtac
     1321 tggcgctgtt tgttggcttt cacttccagc tggaaacgcc acgctgggcg gtactgacag
     1381 cggcgattgt tgccgccggt ccggcctttg ctgcgggagg tgaaccgtat tctggcgcta
     1441 ttcgctatcg tggctttttg cgcatcatcg gcacatttat tggctgtatt gccggactgg
     1501 tgatcatcat tgcgatgatc cgcgcaccat tattgatgat tctggtgtgc tgtatctggg
     1561 ccggtttttg tacctggata tcctcgctgg tacgaataga aaactcgtat gcgtgggggc
     1621 tggccggtta taccgcgctg atcattgtga tcaccattca gccggaacca ttgcttacgc
     1681 cgcagtttgc cgtcgaacgt tgtagcgaga tcgttatcgg tattgtgtgt gcgattatgg
     1741 cggatttgct cttttctccg cgatcgatca aacaagaagt ggatcgagag ctggaaagtt
     1801 tgctggtcgc gcaatatcaa ttaatgcaac tctgtatcaa gcatggcgat ggtgaagttg
     1861 tcgataaagc ctggggcgac ctggtgcgac gcaccacggc gctacaaggc atgcgcagca
     1921 acctgaatat ggaatcttcc cgctgggcgc gggccaatcg acgtttaaaa gcgatcaata
     1981 cgctatcgct gacgctgatt acccaatcct gcgaaactta tcttattcag aatacgcgcc
     2041 cggaattgat cactgatact ttccgcgaat tttttgacac gccggtagaa accgcgcagg
     2101 acgtccacaa gcagctcaaa cgcctgcgaa gagttatcgc ctggaccggg gaacgggaaa
     2161 cgcctgtcac catttatagc tgggtcgcgg cggcaacgcg ttatcagctt ctcaagcgcg
     2221 gcgttatcag taacacaaaa atcaacgcca ccgaagaaga gatcctgcaa ggcgaaccgg
     2281 aagtcaaagt agagtcagcc gaacgtcatc atgcgatggt taacttctgg cgaaccacac
     2341 tttcctgcat tctgggaacg cttttctggc tgtggacggg ctggacttcc ggcagtggtg
     2401 caatggtgat gattgcggtc gtgacgtcac tggcaatgcg tttgccgaat ccacgcatgg
     2461 tggcgatcga ctttatctac gggacgctgg ccgcgctgcc gttagggctg ctctactttt
     2521 tggtgattat ccctaatacc caacagagca tgttgctgct gtgtattagc ctggcagtgc
     2581 tgggattctt cctcggaata gaagtacaga aacggcgact gggctcgatg ggggcgctgg
     2641 ccagcaccat aaatattatc gtgctggata acccgatgac tttccatttc agtcagtttc
     2701 tcgacagcgc attagggcaa atcgtcggct gtgtgctcgc gttcaccgtt attttgctgg
     2761 tgcgggataa atcgcgcgac aggactggac gtgtactgct taatcagttt gtttctgccg
     2821 ctgtttccgc gatgactacc aatgtggcac gtcgtaaaga gaaccacctc ccggcacttt
     2881 atcagcagct gtttttgctg atgaataagt tcccagggga tttgccgaaa tttcgcctgg
     2941 cgctgacgat gattatcgcc caccagcgcc tgcgtgatgc gccgatcccg gttaacgagg
     3001 atttatcggc gtttcaccga caaatgcgcc gcacagcaga ccatgtgata tctgcccgta
     3061 gcgatgataa acgtcgtcgg tactttggtc agttgctgga agaactggaa atctaccagg
     3121 aaaagctatg catctggcaa gcgccaccgc aggtgacgga accggttcat cggctggcgg
     3181 ggatgctcca taagtatcaa catgcgttga ccgatagtta agtcaaaacc gacgccaaaa
     3241 gcgtcggttt tttcatggct atacttagcg atgcacggca gaactcgccg cgaaacgtga
     3301 cggtggcaac agatgaatat ttataccttt gattttgatg agattgagag tcaggaggat
     3361 ttttatcgtg actttagcca aacctttggt ctggcgaaag ataaggtacg cgatctcgac
     3421 tcactatggg atgtgttaat gaacgatgtc ctgccgctac cacttgagat tgaatttgtt
     3481 catctgggag agaaaacgcg tcgccgtttt ggcgcgttaa tattgctgtt tgatgaggca
     3541 gaggaagagc tggaagggca tttgcgtttt aatgttcgtc attagcgcaa aaaaaagccc
     3601 ccgaaccggg ggcaatatcg tcggacaaga cgatgagggt ttatttgtac agttcagccg
     3661 tagcgtgcca ggtgtcaccg ctacgagctt cagtaatctg gtaggccgtt gcgcctttct
     3721 cttccgcttt tttgttcagc atttcacgca tatccattgg cgaagacgcc acaccactta
     3781 cggatacggt cccgattgct tcacgatttt gtgcttgtgc agcatcaatg gagtcggcag
     3841 cgaatgcacc gaaagagaga acagaaagta cgcttaatgc agcaacagtg gttttgattt
     3901 tcatgatttt tacctcgaca taatctttta gctgggcctt tgtttcgtga cccatatcac
     3961 aaaatcaagt atacactaat tattgagcta attaataccg tgctaactgt ttcagttgat
     4021 actaagtgtt aaaaatgtga attttaataa caaaaaagaa ttaaaaatac ggttatttat
     4081 cgttaattct agttttttca attagataat gaattttgca taatgtgctt tttttacctt
     4141 atgtattcat tgtgtgaatg acatgtcgca gtaaaacgca ctattcgtta aattaatcgc
     4201 aggggaaagc agggggttga gagggataag caacattttc cccgccgcca gaagcgacgg
     4261 ggcagagatt aaagctcctg gtcgaacagc tctaaaatcg cttcatacag gtctttgacg
     4321 gtgaaaccgt tagcaggggt ggtaaagatg gtgtcatcgc cagcgatggt gcccagaata
     4381 ccttctgctt tgcccagtga gtccagcagg cgagcaatta actgcgccgc gccagggctg
     4441 gtatgaatca cgacaactgc atcgttgtag tcgatatcca gcaccagatt tttcaatgga
     4501 ctggaggtgg ttggtacacc cagttcagct ggcaggcagt aaaccatttc cattttggca
     4561 ttgcgtgtac gtacagcacc aaacttggtc aacatccgcg agactttaga ctgattaata
     4621 ttgtcaaagc cttgctcctg caacgcggcg acgatttcgc cctgggagct aaatttctct
     4681 tctttaagta atgctttaaa tgctttaact agttcttctt gcttagccga gcttcgcata
     4741 agtcacccga tatggtggtt gatacaacat tattgtgcat acagatgaat ttttatgcaa
     4801 acagtcagcc ctgaagaagg ctgaaataat gttatgaaag agcgggattt tatcaaattt
     4861 cgttattgag aaatatgcct gcgtcacggc atgcaaattc tgcttaaaag taaattaatt
     4921 gttatcaaat tgatgttgtt ttggctgaac ggtagggtat attgtcacca cctgttggaa
     4981 tgttgcgcta atgcataagc gattgttaat tacgtaactt aggttcctga ttacggcaat
     5041 taaatgcata aacgctaaac ttgcgtgact acacattctt gagatgtggt cattgtaaac
     5101 ggcaattttg tggattaagg tcgcggcagc ggagcaacat atcttagttt atcaatataa
     5161 taaggagttt aggatgaaag tcgcagtcct cggcgctgct ggcggtattg gccaggcgct
     5221 tgcactactg ttaaaaaccc aactgccttc aggttcagaa ctctctctgt atgatatcgc
     5281 tccagtgact cccggtgtgg ccgtcgatct gagccatatc cctactgctg tgaaaatcaa
     5341 aggtttttct ggtgaagatg cgactccggc gctggaaggc gcagatgtcg ttcttatctc
     5401 tgcaggtgta gcgcgtaaac cgggtatgga tcgttccgac ctgtttaatg ttaacgccgg
     5461 catcgtgaaa aacctggtac agcaagttgc gaaaacctgc ccgaaagcgt gcattggtat
     5521 tatcactaac ccggttaaca ccacagttgc gattgctgct gaagtgctga aaaaagccgg
     5581 tgtttatgac aaaaacaaac tgttcggcgt taccacgctg gatatcattc gttccaacac
     5641 ctttgttgcg gaactgaaag gcaaacagcc aggcgaagtt gaagtgccgg ttattggcgg
     5701 tcactctggt gttaccattc tgccgctgtt gtcacaggtt cctggcgtta gttttaccga
     5761 gcaggaagtg gctgatctga ccaaacgtat ccagaacgcg ggtactgagg tggttgaagc
     5821 gaaagccggt ggcgggtcgg caaccctgtc tatgggccag gctgctgcac gttttggcct
     5881 gtctctggtt cgcgcactgc agggcgaaca aggcgttgtc gaatgtgcct acgttgaagg
     5941 cgacggtcag tacgcccgtt tcttctctca gccgcttctg ctgggtaaaa acggcgtgga
     6001 agagcgtaaa tctatcggta ccctgagcgc atttgaacag aacgcgctgg aaggtatgct
     6061 ggatacgctc aagaaagata tcgccctggg cgaagagttc gttaataagt aattgattag
     6121 cggataataa aaaaccggag cacagactcc ggtttttttg tctttcacgc tggccttaat
     6181 tggttgccgg atattcctga atggtgacct gcagcgttaa ctgcttatca tcacgcatca
     6241 ctacaaccgg gatcaccgaa ccagggcgaa tttctgccac ctgatccatc gtctccagag
     6301 cagagatggc cggtttgtta tccaccgaaa taatcagatc gttgacctga atacccgcat
     6361 tcgccgccgg gccgtcaggt gacacttcat taaccacgat cccttgcagt tgatctatac
     6421 caccgccctg cgcgtgcagt ggtgcgatct cgcgtccgcc gataccaatg tagccgcgga
     6481 tcacgcgacc atcgcggatc agcttatcca taattttggt tgctaactgg aaaggaatcg
     6541 caaagccgat accttccggc gtttcgccat cgttactctt atcaaacgac agcgtgttaa
     6601 tgcccatcag ttcgcccagc gagttcacca gcgcgccgcc agagttaccg tggttaatgg
     6661 aagcatcggt ttgtaggaag ttttgccgcc cggtcgggtt cagaccgatt cgacccgtag
     6721 cactaataat cccctgggta atggtctgcc cgaggttgta cgggttaccg atcgccagta
     6781 ccacgtcgcc aatgtgcggt acgcgacgtg cattaattgg aatggtaggt aaaccgccag
     6841 tggcattaat tttaagtacc gccagatcgg ttagagagtc agatcccacc agcaatgctt
     6901 caaatacacg tccatcctgt aaggcgacga tgatctgatc ggcgtcgttg atgacgtgtt
     6961 tattggtgat gatataaccg cgttgatcca tgattacacc ggatcccagg gtgcggatct
     7021 caagctggtt gtgagagttg gtgttcaaac cacggttgta aacgttaacc accgctggcg
     7081 cggcgcggcg aaccgccaga ttataactgg caggcgtctc atcggtactg tcaaattgcg
     7141 gagtggaaag cgggttaagg ctgcgcagcg aaggcatggc aaccagcaga atagcgccga
     7201 caattaatcc aatcgcaacg gaacgtaaga gcttcacaaa catgatggag gcgtcattaa
     7261 aaaagggaac ggcagcagca taccacgagt taaccggaca tcacacgtca gcctgatgcc
     7321 cggtttacga cattaacgca tcagcagata gatgctttca ttgccgcgta caatttgcag
     7381 ggcgatgatg gccggttttg ccgccagcac tttacgcatt tcagcaatcg agttcacccg
     7441 atcgcggttg acgccaatga tcacatcgtc tttttgcaag ccagcctgag cagctgggct
     7501 tcctttgaca acttcatcga ttttaatacc tttgccgcca tcttttagct gaccatcgct
     7561 caacgttgca ccttccagcg ctggcgtgat catttcagcg ctggccgacg aagaggtgct
     7621 ggtatcgagc gtcacttcta cttccagtgg tttgccgtta cgcagcaggc caagcttcac
     7681 tttcgtgccc ggctcggtgg tcgcgatacg agagcgcaac tcagcaaagc tattcagcgg
     7741 tttgccgttg aggctggtaa taatatcgcc cgttttgacg cccgctttcg ccgagccaga
     7801 acctggcaac acttcgctga caaacgcgcc acgctgcacg tcaaggttga aggctttggc
     7861 gatatcggca ctcatctcgg tgcctttgat gcctaacaaa ccgcgtttga tttcaccaaa
     7921 gtcgataagc tgctgcgcca gtgttcgcgc catattactg gggatggcaa atccaattcc
     7981 gacgctcccg ccgccaggcg caaggattgc agtgttgatg ccaattaact caccgttaag
     8041 gtttaacagt gcaccgccgg agttaccgcg gttaatggaa gcatctgtct ggataaagtt
     8101 ttccagacct tcaagattca acccgctgcg tcctaatgcg gaaacaatgc cagaggtggc
     8161 ggtttgccca aggccaaatg ggttaccgac cgccacggca aaatcaccga cgcgcaattt
     8221 atcggagtcg gcaatagcga tttgcgttaa tttgctcggg ttttgaattt gcaacagggc
     8281 gatatcgctc tggtcatcgc taccaatcag ttttgcatca aactcgcgcc catcattgag
     8341 ctgaatactg attttctgtg cctgattaat cacatggttg ttggtcagca catagccttt
     8401 actggcgttg atgatgacac cggagcctaa accttcgaag ggttgtgcag gttgatccgg
     8461 taaatcatca ccaaaaaact ttttgaattc ttccgggatt ttctgtccct gactggccgt
     8521 tccttccacc cgtacgctca ccactgccgg aagcactttt tccagcattg gagccagact
     8581 ggggaggggg gcctgatcgg caacctggcc tgggatcgac gcaacggcct gaaatgacgc
     8641 cgagagagtt aacccgacac ttaacgctaa tgcactcaac agctgggttt gttttttcat
     8701 tattcctgct ctcgtacctg aatgataaga aaagagattc aaaacagttt gatgttattg
     8761 aattttcaga cggctaacaa tgagacacca gattaaatga tagtcgggga gggagagaag
     8821 aggagggcgc aatggctgcg cccgaaaaat aaattagtcg cgcttcgcgc cagtacgcag
     8881 caggccggat gcgccttcag aatagtcgcg aggcatctgc accggtgcct gatcgttgct
     8941 ggcttcagac tctgccagac gattacggaa cgggtttgct tcagcagaca gttccggcag
     9001 caggctgctg gagcttttcg ccatgtgctg atacagctgg cgatagtcgt gcgccatggt
     9061 atccagtaat tccgcgctgc gggcaaagtg gctaaccagc tcttcgcgat actcgtccag
     9121 ttcagcttta ttcttttcca gttcgtactg caacgcctgt tgctggcgta gtttacgatt
     9181 accaaaacgc atggccacag caccaataat gatgccgacg actaacccaa ttagcgcata
     9241 ttcccaggtc atgaacatct cccgttgtct tttgtttccg tagggtgttg gcttcaggct
     9301 ccatgcctgc ggctgattat gccactataa ccgttaattc cacagaagtg gaatcccgac
     9361 tgcatatcgc gtagtgtaga acggcctttt tttcgtcaac cgtgaacaac ggcgcaccga
     9421 ttattcaagg aataacaata agatcatgca aagcgttact ccaacatcgc aatacctgaa
     9481 ggcgctcaat gaaggcagcc atcaacccga cgacgttcaa aaagaggccg tcagccgctt
     9541 ggaaattatt tatcaggaac tcatcaatag cacgccacca gcccccagga cgagtgggct
     9601 aatggcgcgg gtcggtaagc tgtggggtaa acgggaagac acaaagcata cgccagtgcg
     9661 tggcttatat atgtggggcg gtgtaggacg cgggaaaacc tggctgatgg accttttcta
     9721 tcaaagcctg ccgggagagc ggaaacagcg cctgcacttt caccgtttta tgctgcgggt
     9781 gcatgaagag ctaactgcct tacaggggca gaccgatccg ctggaaatta ttgctgatcg
     9841 ttttaaagca gaaactgacg tgctctgttt tgacgaattt tttgtctctg atattaccga
     9901 tgccatgcta cttggcggcc tgatgaaagc cctgttcgcc cgcggtatta ccctggtagc
     9961 gacgtcaaat attccgccgg acgaacttta tcgaaatggc ttgcaacgtg cgcgttttct
    10021 gcctgcaatc gatgccatta aacagcattg tgatgtaatg aacgtggacg ctggtgttga
    10081 ttatcgtctg cgtacactca ctcaggcgca tctgtggctt tcgccactcc acgatgaaac
    10141 ccgggcgcaa atggataaac tatggttggt gctggcgggg gggaaacgag aaaattcacc
    10201 gacgttagaa atcaaccatc ggccattagc gacaatgggc gtcgagaacc agacgctggc
    10261 ggtctctttt actacgctgt gcgtcgacgc ccgcagtcag catgactata ttgcgctctc
    10321 acgtctcttt catacggtca tgttgtttga tgtaccagtt atgacgcggt tgatggagag
    10381 cgaagcgcgg cgctttattg cgctggtgga tgagttttac gagcgccatg tcaaattagt
    10441 ggtgagtgca gaagtgccgc tgtatgaaat ttatcagggc gatcggctga agtttgagtt
    10501 ccagcgttgc ctgtcacgtc tgcaagagat gcaaagcgaa gagtatctga agcgcgagca
    10561 tttagcgggt taaaacctgc cacaaatcac aaaaaggggt cgatctttga ccccgacttc
    10621 tctataatcc tgcgacccca cgttacaaga aagttttttt cccaaaactt tttgtgtgct
    10681 ggcataggct attcgaaggg gtaggtttgc cggactttgt cgtgtgaacc tcaacaattg
    10741 aagacgtttg ggtgttcacc aacgtgtaac tatttattgg gtaagctttt aatgaaaact
    10801 tttacagcta aaccagaaac cgtaaaacgc gactggtatg ttgttgacgc gaccggtaaa
    10861 actctgggcc gtctggctac tgaactggct cgtcgcctgc gcggtaagca caaagcggaa
    10921 tacactccgc acgtagatac cggtgattac atcatcgttc tgaacgctga caaagttgct
    10981 gtaaccggca acaagcgtac tgacaaagtg tactatcacc acaccggcca catcggtggt
    11041 atcaaacaag cgacctttga agagatgatt gctcgccgtc ctgagcgtgt gattgaaatc
    11101 gcggttaaag gcatgttgcc aaaaggcccg ctgggtcgtg ctatgttccg taaactgaaa
    11161 gtttacgcgg gtaacgagca caaccacgcg gcacagcaac cgcaagttct tgacatctaa
    11221 tcgggattat aggcaatggc tgaaaatcaa tactacggca ctggtcgccg caaaagttcc
    11281 gcagctcgcg ttttcatcaa accgggcaac ggtaaaatcg taatcaacca acgttctctg
    11341 gaacagtact tcggtcgtga aactgctcgc atggtagttc gtcagccgct ggaactggtc
    11401 gatatggttg agaaactgga cctgtacatc accgttaaag gtggtggtat ctctggtcag
    11461 gctggtgcga tccgtcacgg tatcacccgc gctctgatgg aatacgacga gtccctgcgt
    11521 tctgaactgc gtaaagctgg cttcgttact cgtgacgctc gtcaggttga acgtaagaaa
    11581 gtcggtctgc gtaaagcacg tcgtcgtccg cagttctcca aacgttaatt ggcttctgct
    11641 ccggcagaaa acaatttgcg aaaaaacccg cttcggcggg tttttttatg gttaaattct
    11701 gaatcagcgt aaaaaatgga aagttgcttt ttgctgccgt ctgacagaca ggcaaaacaa
    11761 aaaccacatc gccaataagg gactaagtca actatttcag actaaagcgc atctcttttt
    11821 ccccatttcc ggcatcgact caccacaaag gtcgcaaaat ctggtaaact atcatccaat
    11881 tttctgccca aatgtcgggt attgctcatt ttttgtttga ttttcgaaca aagagagtcg
    11941 ttccttactg ggtaacacaa cttctgactg gccacctggt ggctggtagc agtaaaaatt
    12001 ctgactatac ctggaggttt tcatggctgt cgctgccaac aaacgttcgg taatgacgct
    12061 gttttccggt cctactgaca tctatagcca tcaggtccgc attgtgctgg ctgagaaagg
    12121 tgtaagtttc gagatcgaac acgtggaaaa ggacaatccg cctcaggatc tgattgacct
    12181 caacccgaat cagagcgttc cgaccctggt ggatcgtgag ctgaccctgt gggaatctcg
    12241 catcattatg gaatatctgg atgagcgttt cccgcatccg ccactgatgc ctgtttaccc
    12301 ggtagctcgc ggtgaaagcc gtttgtacat gcatcgcatc gaaaaagact ggtacacgct
    12361 gatgaacacc atcatcaacg gttcagcttc tgaagcagat gccgcacgta agcaactgcg
    12421 cgaagaactg ctggcgattg cgccggtctt cggtcagaag ccgtacttcc tgagcgatga
    12481 gttcagcctg gtcgattgct accttgctcc gctgctgtgg cgtctgccgc aactgggtat
    12541 cgagttcagc ggcccgggtg cgaaagagct gaaaggctat atgacccgcg tctttgagcg
    12601 tgactctttc cttgcttctt taactgaagc agaacgtgaa atgcgtctgg gccggagtta
    12661 atctgtatgg atttgtcaca gctaacacca cgtcgtccct atctgctgcg tgcattctat
    12721 gagtggttgc tggataacca gctcacgccg cacctggtgg tggatgtgac gctccctggc
    12781 gtgcaggttc ctatggaata tgcgcgtgac gggcaaatcg tactcaacat tgcgccgcgt
    12841 gctgtcggca atctggaact ggcgaatgat gaggtgcgct ttaacgcgcg ctttggtggc
    12901 attccgcgtc aggtttctgt gccgctggct gccgtgctgg ctatctacgc ccgtgaaaat
    12961 ggcgcaggca cgatgtttga gcctgaagct gcctacgatg aagataccag catcatgaat
    13021 gatgaagagg catcggcaga caacgaaacc gttatgtcgg ttattgatgg cgacaagcca
    13081 gatcacgatg atgacactca tcctgacgat gaaccaccgc agccatcacg cggtggtcga
    13141 ccggcattac gcgttgtgaa gtaatacaaa aacaggccct ggcggcctgt tttgtctttt
    13201 aatgacttaa taaaatagcg tgattgtggc aatcatattc accacgaaac cgactgccat
    13261 ggggatcgct gtccgcttca ccaccgcaaa aggcgaaacg cctgcaattc ctgacgtaac
    13321 gaccaccacc gcagtaatcg gcgaaaccgc gcgctagcgt cgtggcaaaa tgcatcggca
    13381 taatcattac aaccgctggt acatgtagtc cggctgcgat attcggaata agactggcaa
    13441 atgacataaa cggcgcattg ccagagccca tcacaatggc acaaatggca atgaccagcg
    13501 ccataataat catcacgcca ataccgccca gaccagaatg ctccgcaccc ctgataaccg
    13561 catcgacagt gccaatcgtc gttaagcctt tcgcaaatat ttcacccgcg accaccagcg
    13621 ttaccacgtt ggcaaactgc gtacccatgc cgtcaaaaaa cgcctgcaca tcgtccattg
    13681 tttcgcgcaa gttatgcttg cggaagaact caacaaacat cgtcaaactc agtaacatca
    13741 ccaccaccag atgcagttct gactgcatca gcccgatgtg ggcaaggaat agcgagccga
    13801 gcatcaggat taacggcatc acaggtaaaa tggcgtaata gagcggcggg acattatcga
    13861 gagctttttg ctctgcctgt tcgtgattga tatttttatc ttttttgtca aaagcgcgtt
    13921 gcacgaaaaa gtgggagatc gccaccgaga taatgacgca agaggcgacc ggaagctggt
    13981 agtggaagaa gtaagtggca attttcattc ccgctacctg ggctgcaaaa atggagttcg
    14041 tttccagaat cccccactca atggacattg tggttgcgat aaccgccacc gcagagagac
    14101 ggcttactcc cagactcacc agtgtcggaa ataaggtgac catcagcaac atacccaggc
    14161 cggaggcgct ggtaataaac tgcgccatga tttggccgat gacgtaagtt gccgacagaa
    14221 taatatacgg cgagcgaatg agttttaacg ggcggcttaa caagctcacc atcgcgcgac
    14281 tggctcctgt gcgctccatg tagcgggcat aaccgcccac cgccataatc gacagcccca
    14341 atccggcaat gcggttggac agcatgcgca ggatttcatt ataaatatcg acgatcaagt
    14401 agccgctgct tttggcggga tcgagtacac cacctaaccc taaccagaca ccgcacatca
    14461 tcaggaagat acctgcagcc agcaccacct gaggtttgta gtttttcagg atcaaatagc
    14521 ccatcgtaat taatacgatg acagatataa ttatgccgaa cattgttgaa ctccgtgtca
    14581 aaagaaaagg gtcaatccca taaacggcag attgaaaaca atgatgttat attttttgca
    14641 agtctattta tggtgcggat gtcgtgtttt taattgtagg tgaggtgatt tttcattaaa
    14701 aaatatgcgc tt
//
LOCUS       DAZKIP010000106        14537 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZKIP010000106 DAZKIP010000000
VERSION     DAZKIP010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.107"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..2243)
                     /locus_tag="ACHOUK_003560"
     CDS             complement(<1..2243)
                     /locus_tag="ACHOUK_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFP9898933.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASVHAQTVEKSAQAHERMAREVEQTRQRMEALSQK
                     MREEQAQAMALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGIGQQ
                     DYLALISEVTAKTRVLTQAEEEATRQKVAFIRQLKEQATRQNLSSSELLRAKAAQLGV
                     SSAAEVYIRKMEQAGKATHSLGLKSAAARQEIGVLIGELARGNLGALRGSGITLANRA
                     GWIDTLMSPKGMMPGAVIGGIAAAVYGLGKAWYDGQKEGEEFNRQLSLTGHYAGVTAG
                     QLWTLSRAISGNGITQHAAAGALAQVVGSGAFRGNDIGMVARAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNELHFLTATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     RRTADIDNNLNALGSTLKYLSDLWSRFWDAAMNIGREDSLDEQIAALQEKVSRAKRLP
                     WTASSSQVEYDQQRLNDLQEKKRQKDLQDAKEQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQREDERYEKALASGKKKTRETRNDEATRLLL
                     QYSQQQAQVEGQIAAARQSAGIATERMTEARKQLLALQQRISDLDGKKLTADEKSVLA
                     RKDELIQALTLLDVKQQELQKQTALNELKKKTIQLTSQLAEEERAQRQQHDLDIATVG
                     MGDQQRQR"
     gene            complement(2215..2544)
                     /locus_tag="ACHOUK_003561"
     CDS             complement(2215..2544)
                     /locus_tag="ACHOUK_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFP9898934.1"
                     /translation="MQFVMRLAREFRRADWRRMLSEMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            complement(2553..2939)
                     /locus_tag="ACHOUK_003562"
     CDS             complement(2553..2939)
                     /locus_tag="ACHOUK_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFP9898935.1"
                     /translation="MFLKQGTFNYEKQSVVLSELSGLQRIEYLAFVQQRTAKFDAEEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASVITTWSYDALGAGAEM
                     VLSLSGMGAIENAGDLEHEVLTPEKS"
     gene            complement(3000..3743)
                     /locus_tag="ACHOUK_003563"
     CDS             complement(3000..3743)
                     /locus_tag="ACHOUK_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9898936.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASASVNVGATTTLTFTVKPDGASDKAISVHSTDPQTATVTLNGLVATVKG
                     VKQGSVSIVGMTADGNFVAVAAVTVSAAG"
     gene            complement(3754..4155)
                     /locus_tag="ACHOUK_003564"
     CDS             complement(3754..4155)
                     /locus_tag="ACHOUK_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFP9898937.1"
                     /translation="MNRHTQIRQVVLARLREQCGDSATFFDGLPAFVDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVSALSGLID
                     TLIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            complement(4152..4730)
                     /locus_tag="ACHOUK_003565"
     CDS             complement(4152..4730)
                     /locus_tag="ACHOUK_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9898938.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAGSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLGTARVRLAQR
                     GGKLQYRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPLSGPLTQ
                     AFEDARDRIIAAEMPKQLGYALKQQLRLWLTR"
     gene            complement(4742..5017)
                     /locus_tag="ACHOUK_003566"
     CDS             complement(4742..5017)
                     /locus_tag="ACHOUK_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFP9898939.1"
                     /translation="MSDPFSRLAARMDAITVRKMGKTASINDVDMTVIPGETLAELNA
                     LSGPAVSLVVFSSGYRPRRGDRVVYDGQQWTVTRHERFNGKPMIFIE"
     gene            complement(5010..5333)
                     /locus_tag="ACHOUK_003567"
     CDS             complement(5010..5333)
                     /locus_tag="ACHOUK_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HFP9898940.1"
                     /translation="MAKNFVEEGKTVAIVASAAISSGDLVQVGDVFAVALTDIPQGET
                     GDGMTEGVFMLPKLKTDDMKTGKKVYLKSGKVQLTNSGSDPLVGVVWADAGTSAEEVP
                     VKLNV"
     gene            complement(5420..7447)
                     /locus_tag="ACHOUK_003568"
     CDS             complement(5420..7447)
                     /locus_tag="ACHOUK_003568"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFP9898941.1"
                     /translation="MPQRNDRSRSTPTTSPKNNSWFRMQAGHQSDADIYIYDEIGFWG
                     VTAKQFISDLNALGDITHINLNINSPGGDVFEGIAIFNALKTHGASITVYVDGVAASM
                     ASVIAMVGKPVIMPENSFMMIHKPFGFTGGDAEDMRTYADLLDKVEAVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAHGFADQVTPAVKAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRVQDDGPAASRTPVQAAAPVVDENSIRAQVLAEQKARVNGINDLFAMFG
                     GRYQTLQAQCLADPECSLEQAREKLLNEMGRESTPSNKNTPAHIYAGNGNFVGDGIRQ
                     ALMARAGFEKTERDNVYNGMTLREYARISLTERGIGVSSYNPMQMVGAAFTHSTSDFG
                     NILLDVANKAILQGWEDAPETYEQWTRKGQLSDFKIAHRVGMGGFSALRQVREGAEYK
                     YVTTGDKQATIALATYGELFSITRQAIINDDLNMLTDVPMKLGRAAKSTIADLVYAIL
                     TSNPKISTDNVSLFDKAKHANVLESAAMDVASLDKARQLMRVQKEGERHLNIRPAFVL
                     VPTAMESVANQVIRSSSVKGADINAGIINPVKDFATVIAEPRLDDNSQTTFYLAASKG
                     SDTIEVAYLNGVDTPYIDQMEGFSVDGVTTKVRIDAGVAPVDHRGLVKCTA"
     gene            complement(7392..7727)
                     /locus_tag="ACHOUK_003569"
     CDS             complement(7392..7727)
                     /locus_tag="ACHOUK_003569"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9898942.1"
                     /translation="MASGVIRLPRDLDRSSLYTAVYSGPVMPWIDPVKEAEAWKIQIR
                     GGAATESDWVRAGGRNPDDVKRRRKAEIDENRKLDLVFDTDPASDKGGSSAATKRQEP
                     QHTDDQSEE"
     gene            complement(7849..8973)
                     /locus_tag="ACHOUK_003570"
     CDS             complement(7849..8973)
                     /locus_tag="ACHOUK_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9898943.1"
                     /translation="MGAPPCGSDYTTTGASRVPAGEVLMAILDDVIGVFSPGWKAARL
                     RSRAVILAYEAVKTTRTHKARRENRTADQLSQYGAVSLREQARYLDNNHDLVIGVFDK
                     LEERVVGKNGIIVEPHPVLRNGAIARDLAAEIRTRWSEWSVSPEVTGQFTRPMLERLM
                     LRTWLRDGEVFAQMVSGRINSLTPSAGVHFWLEALEPDFIPMTSDESNRLNQGVFVDD
                     WGRPEKYLVYKSRPLSGRQMETKEVDAERMLHLKFVRRLHQMRGTSLLSGVLIRLSAL
                     KEYEDSELTAARIAAALGMYIRKGDGQSYEPDGNGSKDKERELTIQPGIIYDDLKPGE
                     EIGMVKSDRPNPNLETFRNGQLRAVAAGSRLNFFQYSAQL"
     gene            complement(8901..9113)
                     /locus_tag="ACHOUK_003571"
     CDS             complement(8901..9113)
                     /locus_tag="ACHOUK_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9898944.1"
                     /translation="MNQNDIEAMIQRYTEAEMAVLDGKSVTFNGQQMTMENLSEIRQG
                     RQEWERRLAALITRRRGHPGYRLARF"
     gene            complement(9110..11212)
                     /locus_tag="ACHOUK_003572"
     CDS             complement(9110..11212)
                     /locus_tag="ACHOUK_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFP9898945.1"
                     /translation="MLNQETAKAARTDSGYILRAPRRMRVADAVAQYMRVPMGAGNSV
                     PWDPLVAPYVIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWVIYNVICDPADMLII
                     QMTEEKAREHSKKRLARTFRVSPEVVSRLSPNKNDNNVYDRTFLAGNYLKIGWPSVNI
                     MSSSDYKCVALTDYDRFPEDIDGEGDAFSLASKRTTTFMSSGMTLVESSPGRDVKDVK
                     WRRTSPHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPCGDVVAGFRDIADPVLAS
                     EAAYIQCPSCSGRIMPEQKRELNGRGVWLRDGESINADGSRYGDPRRSRIASFWMEGP
                     AAAYQTLSQLVYKLLTAEQEYETTGSEETLKTVINTDWGLPYLPRASMEQRKSELLEQ
                     RAEPVPSSSVPDGVNFLVATVDVQAGRHRRFVVQVTGYGSRGERWIIDRYNITQSLRG
                     DSDGESQRIDPASYPEDWDVLLTDVFHKSWPLASDPSQQMRLMAMAVDSGGEDGVTDN
                     AYKFWRRCRRDGLGKRIYLFKGDSIRRAKLITRTFPDNTGRTGRRAQAAGDVPLWLLQ
                     TDALKDRVNNALWRDSPGPGYVHFPDWLGSWFYDELTYEERSSDGKWSKPGRGANEAF
                     DLMVYAEALVILHGYEKIRWPDAPGWASRETWLESVPDSTEPSPSPEPVSTPVKKQKR
                     KKTVTDDVNPWLTSGGWL"
     gene            complement(11212..11703)
                     /locus_tag="ACHOUK_003573"
     CDS             complement(11212..11703)
                     /locus_tag="ACHOUK_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFP9898946.1"
                     /translation="MDHELKNLVLNINQLAALSGLHRQTVVARLKNIRPAGGHDKLKL
                     YRLTDILTEFMGLPPPVAEGEMDPHERKAWYQSERERLKFEQETAQLIPASDVRREFA
                     IWAKAVVQVLETLPDILERDCGLQPAAVSRVQSIIDDLRDQIALRVTEAGADDEEELQ
                     QEE"
     gene            complement(12378..12530)
                     /locus_tag="ACHOUK_003574"
     CDS             complement(12378..12530)
                     /locus_tag="ACHOUK_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9898947.1"
                     /translation="MPSMIAIILLIILHIWLCRQGGAHFWSESWLNISLLMLDIEHLA
                     RGKGLR"
     gene            complement(12518..12985)
                     /locus_tag="ACHOUK_003575"
     CDS             complement(12518..12985)
                     /locus_tag="ACHOUK_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFP9898948.1"
                     /translation="MSRVTAIISALVICIIVCQSWAVNHYRDNAIAYKEQRDKKVSEL
                     KQATATITDMQQRQRAADALDAKYTKELADAKAKNDALRRKLDNGGRVFVKGKCPVPS
                     SDETSSASGMGNDATVELSPVAGRNVLGIRDGIIRDQTALRTLQEYIRTQCLR"
     gene            complement(12982..13479)
                     /gene="rrrD"
                     /locus_tag="ACHOUK_003576"
     CDS             complement(12982..13479)
                     /gene="rrrD"
                     /locus_tag="ACHOUK_003576"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFP9898949.1"
                     /translation="MSPSLRKAVAAAIGGGAAAIASVLITGPSGDDGLEGVSYIPYED
                     IVGVWTVCHGHTGKDIIPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(13479..13694)
                     /locus_tag="ACHOUK_003577"
     CDS             complement(13479..13694)
                     /locus_tag="ACHOUK_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP9898950.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            13837..14235
                     /locus_tag="ACHOUK_003578"
     CDS             13837..14235
                     /locus_tag="ACHOUK_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9898951.1"
                     /translation="MLHIISPRGISPRQRRLFYPFSSALALFYYAAAFTSGTMLSIST
                     QYVVLLVTNIITINRHRISPVYRNRVKKPEPESNHRMTICQWQVIKKRPRHAQPELTR
                     KMIEGNNISDVRMAPPAKFCNDQTELATCP"
     gene            complement(14316..14474)
                     /locus_tag="ACHOUK_003579"
     CDS             complement(14316..14474)
                     /locus_tag="ACHOUK_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFP9898952.1"
                     /translation="MFYKLCLLAVVILLLVMVMMDFTSRIMLVLTDGALVCGIVVLLW
                     PMMKEQNE"
BASE COUNT         3340 a         4294 c         3355 g         3548 t
ORIGIN      
        1 cgctgtcgct gctgatcacc catacccacc gtggcgatat ccaggtcatg ttgctgacgc
       61 tgagcgcgct cttcttcagc cagttgactg gtcagctgaa ttgttttttt cttcagctcg
      121 ttgagtgccg tctgtttctg aagctcctgc tgttttacat ccagcagcgt cagtgcctga
      181 atcagttcat ctttacgggc cagcacactc ttttcatctg ccgtcagttt tttcccgtcc
      241 aggtcgctga tgcgctgctg cagagccaga agctgtttac gcgcttctgt catcctttcc
      301 gtggcaatgc ctgctgactg tctggcagca gcaatctgtc cttccacctg tgcctgttgc
      361 tgactgtact gcagcaataa ccgggtggcc tcatcattac gggtttcgcg tgtttttttc
      421 ttaccggatg ccagggcttt ctcgtaacgt tcatcttcac gttgtatcgc cgcatccctg
      481 acagcctgat cggcgtactg catggcatta atacgcgcta tttcacgctg atgtcgtgct
      541 gcttccgttt cattcatccg gttcagtgca gcattttcag cattacggcg tttctgttgc
      601 tcctgataat tccgctctgc ctgctctttt gcatcctgca aatccttctg gcgttttttc
      661 tcctgaagat cgttaagacg ctgctgatcg tattcaacct gagaagatga tgccgtccag
      721 gggagtcttt tcgcccgcga cactttctcc tgtaaagcgg caatctgttc atccagcgag
      781 tcttcacgac caatattcat ggccgcatcc cagaaacgac tccacaaatc agacagatac
      841 ttcagcgtac tgccaagcgc attgaggtta ttatcaatat ccgcagtacg ccgaccggtt
      901 tcctctgcca gtgcagacat ggctatccgt gcagcatcac tggaccgccc ctgctcccca
      961 aggacgcgta tctgctcaag ctgagtggca gtaagaaaat gcagctcatt gtccagagcc
     1021 ttcgcggcat ttacaggatc atccttcagc cgcttaaact gatttatggt atcgctgacc
     1081 gactggccaa ccgatcgctc catctgtgcg gcagctctcg ccaccatacc gatatcgttt
     1141 ccacgaaatg caccactccc caccacctga gccagcgcac cggctgcagc atgttgcgtg
     1201 ataccattcc cggaaatagc acgactgagc gtccacagct gcccggcagt gactccggca
     1261 taatgccccg tcagcgacag ctggcggtta aattcttccc cctccttctg accgtcatac
     1321 caggctttac ccagaccata gacagccgcg gcaataccgc caataaccgc tcctggcatc
     1381 atgcctttcg gtgacatcag tgtgtctatc cacccggcac ggttagccag cgttattccg
     1441 gatcccctca gcgcacctaa attgccgcgg gccagttcac ctatcagaac gcctatctcc
     1501 tgacgggctg ctgcactttt cagacccagc gaatgcgtgg cttttcctgc ctgctccatt
     1561 ttgcggatat acacttctgc agcactgctt acccccagct gggcagcctt agcacgaagc
     1621 aactcagaag aagaaagatt ctggcgggtt gcctgctctt taagctgacg gataaacgcc
     1681 actttctgtc gggtagcctc ttcctcagcc tgtgtaagaa cacgggtttt cgccgtaacc
     1741 tcagaaatca gcgccagata atcctgctga ccaatcccgc cactgtttct ggcctgtcgg
     1801 atctgttgct gaatacgctg taattcctgc agccccgcac cggcctgttt cacactgtca
     1861 atctgacgat aaaacgcagc agccgcttta tcctgagcct ccgccagagc catggcctgc
     1921 gcctgttcct cgcgcatttt ctggctcagt gcctccatac gctggcgggt ttgctccacc
     1981 tcgcgggcca tgcgttcatg agcctgtgcg ctcttctcca ccgtctgcgc atggacggat
     2041 gcggctgttg cagccgaaga agccgcctgc attgtctgcc gggcggcctg agtctgacgc
     2101 tccataaaac gctgcatacg ggcagaagac cgttctgcat cgctggctgc accattcaga
     2161 aggtttttga tacggggaat ttcattttta aactctgccg catcaatccc caaatcaatg
     2221 accaggttgg ctatctggtc cataacgcac acctccggaa ataccttccc caagatgcat
     2281 cagttcttcg tccgttcgct ccggtatccc gttctcttcc ggtaaaaggc tgaaatcagc
     2341 caccgcagca tcactgctac cggacaccat tctcacgatc aatgccttca gcgaggcaaa
     2401 ctgcgcatcc atccacacat cactgaagct ctgcatccgg aaataatcgc cccactcacc
     2461 aagctcagtg gccgacattt ccgacagcat ccgccgccag tctgcccgcc ggaactcccg
     2521 ggcaagccgc atgacaaact gcatttcccg cgtcaggact tttccggcgt cagcacctca
     2581 tgctccaaat ccccggcatt ctcaatggct cccataccgc tcagcgacag aaccatctcc
     2641 gcccccgctc ccagggcatc atacgaccat gttgtaataa cggatgcgca aagcgtctca
     2701 acatcctgag actgttccgc attccacagt gagcgggaaa ccagccaggc attgatatcc
     2761 atccccatcc gcagaaaagc aatctgtcgt tcagcctccg gcagttctcc ctcttcggca
     2821 tcaaactttg ccgttcgctg ctgaacaaac gccagatatt caattctctg cagcccggac
     2881 agctcactga gcaccacgga ctgcttttca taattaaacg tgccctgttt cagaaacatc
     2941 atgttctcca cctgcaaaaa agccccggat aaccggggca aatgatgagt atcgtcctgt
     3001 taacctgcag cgctgacagt caccgcagcc acagccacaa aattcccgtc agcggtcatg
     3061 cccacaatgc tgacgctgcc ctgcttcacg cctttcaccg tggccacaag tccgttcagg
     3121 gtcacagtgg ctgtctgtgg atctgtcgaa tgcacactga tcgctttgtc actggctccg
     3181 tcaggtttta ctgtaaaggt cagcgtggtg gttgctccca cattgacact ggcggatgcc
     3241 ggtgccaccg tcagcccggt aacgcccaca gtttcagtgc cctcctctgc cagatatgga
     3301 cgccccacac cgctgatttt cactgtgcgg gtcatcacgt cttttgaggc aatggtttta
     3361 cccagtgagc tcagccagcc gcggaaaaca tcaacagtgc cgttgggata tttgatacga
     3421 aacgcgcaga cttcaccgga gtcgaacaac tgaaccagtt ttttctgccc gctgtcaccc
     3481 ggacgccagg ccagcgtcgc cgaagtatca ccaacggatt tctgcccctg ggttgtcgtt
     3541 ttccagtctg catcttcatc atcgagataa gtgtcatctt ctgcatcagc ggtcatttcg
     3601 ccaggttgca gatccttcac catcgcaaga cgcagccagt cagtgtccga caaagggttc
     3661 gcaaacgcat cgccgttgcc ggtatacatc cagaacgtcg tccccgcacc tttcgtcttt
     3721 gccagtggat ttggcgtggt cattaccacc tccttaattc gtgtacgtga tctggtacgt
     3781 gatttccgcc atcgcccagg tggccatctc attatcacgt tgatagttaa aaccgagtgg
     3841 gatcagggtg tcgatgagtc cggaaagtgc cgatacatca ttcagggccg ggaaaatggt
     3901 gctctccatc cacatatcca gctctgaatc cggtgcctgt gcccggatga agacagcaat
     3961 atgcagaaca gcctgccagt catcttcatc cgtcattttt ccggtgtact gagcatcact
     4021 cagccacacc gccacggcag gcagttcctg cgcatcaaca aatgccggaa gcccgtcaaa
     4081 aaacgtggcg ctgtctccac actgttcccg aaggcgtgcc agtacgacct ggcggatttg
     4141 tgtatgtcgg ttcatcgggt cagccataac ctcagttgtt gtttcagtgc ataccccagc
     4201 tgtttcggca tttccgcagc aatgatgcgg tcgcgggcat cttcaaatgc ctgtgtcagc
     4261 ggtccggaca gcgggatttt caccacatca atggggtaac gatttttgcc atcaatacgc
     4321 cgcatcacat gccagcgacc attcgccagt tgctgaataa acgcatcccg gaaaagatat
     4381 ttacccacct tcagcacact gccacggtac tgcagttttc caccacgctg ggccagtctg
     4441 acccgggctg tccccagctt aatggcgggc agattgcccc ggttaacgcg gatcctggcc
     4501 gtcatttttc ctgacggact ggctttaaac acccggacac gctgacgaac cagtttcagg
     4561 gggatccctt tcacctggtt atctcccgca acggtattcc cggcaacctg ccgggtggca
     4621 accgagcccg ctttctgtgc cacacggttt atcgcccatg cgctggcctg tggcaccata
     4681 cgggtatcaa ggctgttcag attgcggatg gcattctcaa gccccttcat cccacacctc
     4741 tttactcaat aaagatcatt ggcttaccgt taaagcgttc atgccgtgtg accgtccatt
     4801 gttgtccgtc ataaacaacg cgatccccgc gccgtgggcg gtatcccgaa gaaaacacca
     4861 ccagagagac cgcaggtccg gacagagcat tcagctctgc cagtgtttct cccgggatca
     4921 cagtcatatc gacatcatta atcgaggctg tctttcccat ctttctgacc gtgatagcat
     4981 ccatacgcgc tgccagccgg gaaaagggat cagacattga gttttaccgg cacttcttct
     5041 gcactggttc cggcatctgc ccagacaacc ccgaccagcg gatcagagcc gctgttagtc
     5101 agctgaactt ttcctgactt cagataaacc ttcttacccg ttttcatgtc atccgttttc
     5161 agcttaggca gcataaacac accttcggtc atgccgtcgc ctgtttcacc ctgtggaata
     5221 tcggtcagcg ccaccgcaaa aacatcgccc acctgcacca gatctccgct gctgatggct
     5281 gcactggcaa caatcgccac cgtttttcct tcttctacaa aattctttgc cataactgtc
     5341 tccgcacagc cccgttcagg ggctgatttc aggtacaaaa aaagccctta cgggccatca
     5401 gagttgttgt ctgcgacgtt tacgccgtac atttcaccag accgcggtga tcaactggcg
     5461 cgacaccggc gtcaatacgc actttcgttg tcacgccatc cacactgaag ccctccatct
     5521 gatcaatata tggcgtatcc acaccgttga gataagccac ttcaatcgta tcggagcctt
     5581 ttgacgcagc caggtagaag gtggtctggc tgttatcatc aagacgaggc tctgcaataa
     5641 cggtcgcaaa atctttcacc gggttaataa taccggcgtt aatgtcagcc cccttgacac
     5701 ttgaggagcg aatgacctgg ttagcaacag actccatcgc cgtcggtacc agtacgaacg
     5761 caggacgaat attcagatga cgctccccct ctttctgaac gcgcatcaac tggcgggctt
     5821 tatccagcga tgccacgtcc attgcagcgc tctccagtac gtttgcatgt ttcgctttat
     5881 cgaacagact tacattatct gtggagattt tcgggttaga cgtcagaatg gcataaacca
     5941 gatcggcaat agtggatttc gccgcacggc ccagtttcat cgggacatcg gtcagcatat
     6001 tcagatcatc attgataatg gcctgacggg tgatactgaa cagctcgcca taggtcgcca
     6061 gtgcaatagt ggcctgttta tctccggtgg tgacgtattt atattccgcc ccttcacgca
     6121 cctgacgcag agcactgaag ccccccatac ccacacgatg ggcaatttta aaatcagaca
     6181 actgaccttt ccgcgtccac tgttcatagg tttcaggggc atcttcccag ccctgcagaa
     6241 tggctttgtt cgcaacatcc agcagaatat taccgaagtc agacgtactg tgtgtgaacg
     6301 ccgcaccgac catctgcatc gggttataac tggaaacccc aataccccgt tcagtcagtg
     6361 aaatacgggc atattcacgc agggtcatcc cgttgtagac attatcacgt tcggtttttt
     6421 caaatccggc acgcgccatc agcgcctggc ggatcccatc ccccacaaaa ttaccgtttc
     6481 cggcataaat atgagccggg gtatttttat tagatggcgt ggactcgcgc cccatctcgt
     6541 tcaacagctt ttcgcgggcc tgctccagcg aacattcagg atcggcaaga cactgagcct
     6601 gcagcgtctg ataacgcccg ccaaacatgg caaacagatc attaataccg tttacacgcg
     6661 ctttttgctc tgccagtacc tgcgcacgga tactgttttc atccaccaca ggtgctgctg
     6721 cctgcactgg cgtccgggag gctgcaggtc catcatcctg tacgcgtgga gcactgttgc
     6781 gtggcggagt aatcatgttt cgaatggatt ccggcatctt tttaaattcc tctgtacgtt
     6841 ttgactgaat acatgccatt gccttaacgg ctggcgtcac ctgatcagca aatccatgtg
     6901 ccagacattc ggcaccggac atccaggtct catccgccag catggcagca atttcatcgg
     6961 tggttttccc ggttttctgt gcataagcgg gtaacagaac cgcctcaact ttatcgagca
     7021 ggtcggcata ggtgcgcatg tcctccgcat caccgcccgt aaagccaaat ggtttatgaa
     7081 tcatcatgaa ggagttttcc ggcataatga ccggttttcc caccatcgca atgaccgacg
     7141 ccattgacgc cgccacaccg tcgacataaa cggtaatgga cgcaccatgt gttttcagtg
     7201 cattaaaaat ggcgatgcct tcaaagacat cgccacccgg tgaattgata ttgagattaa
     7261 tgtgggtgat atcgcccagt gcattcagat cactgataaa ctgcttcgct gtaacacccc
     7321 agaaaccaat ctcgtcataa atataaatat ccgcgtcact ctggtgacca gcctgcatcc
     7381 tgaaccagga attattcttc ggactggtcg tcggtgtgct gcggctcctg tcgtttcgtt
     7441 gcggcactgc tgcctccttt atcactggcc ggatcggtat caaataccag atccagcttg
     7501 cggttttcat caatttcggc cttgcgccga cgtttgacat catccggatt acgaccacct
     7561 gcacgtaccc agtctgattc tgtcgctgct ccaccacgaa tctgaatttt ccaggcctca
     7621 gcctccttaa cagggtcaat ccacggcatc accggtccgg aatacaccgc ggtatacagt
     7681 gaagaacggt caagatcgcg gggtagcctg ataacaccgg atgccacagc ctgtttcagc
     7741 caggcacgat acatcgggcg ggtgacggca ccaataaacc agtcctgcag gatcaggtag
     7801 ccatcagtgg attcaaccag ctcctgacgc tgggcgctgt aagtgccgtt atagttgcgc
     7861 gctgtactgg aaaaaattca gacgactgcc cgccgccacg gcacgcaact gaccattacg
     7921 aaaagtttca aggttaggat tggggcgatc cgacttcacc attccgattt cttcgccggg
     7981 tttcagatcg tcgtaaataa tgcctggctg aatggtaagc tcgcgttcct tatccttgct
     8041 gccattacca tccggttcat agctctgccc atcgcctttc cggatgtaca tccccagagc
     8101 agcggcgatc cttgctgcag tcagctcaga atcctcatac tctttcaggg cactgaggcg
     8161 gatcagcaca ccggacaaca aagacgtccc gcgcatctgg tgcagacggc gaacaaattt
     8221 aagatgcagc attcgctctg catccacttc tttggtttcc atctgccgtc cggataaggg
     8281 acggctttta tacaccagat atttttcggg acgcccccag tcatcaacaa acacgccctg
     8341 attcagcctg ttgctctcat cactggtcat gggaataaag tctggctcga gcgcctccag
     8401 ccagaaatga acaccggcag aaggcgtcag gctgtttatg cgcccggaaa ccatctgggc
     8461 aaacacctca ccatcgcgca gccaggtacg cagcatcaga cgttccagca tcggacgggt
     8521 aaactgcccg gtgacttccg gactgacaga ccattcactc catcgggtgc gtatctccgc
     8581 tgccagatca cgggcaatgg ccccattgcg taataccgga tgtggctcga caataatccc
     8641 gtttttcccc accacccgtt cttccagctt gtcaaataca ccaataacca gatcgtggtt
     8701 gttatcaagg taacgggcct gctcacgtaa cgacacggcc ccgtactggc ttaactggtc
     8761 ggcagttcgg ttttcccgcc gggctttgtg tgtccgcgtc gtttttacgg cctcataagc
     8821 caggatcacc gcacgggaac gcagccttgc cgctttccat cctggtgaaa aaacgccaat
     8881 cacatcatca agaattgcca tcagaacctc gccagccggt acccgggatg cccccgtcgt
     8941 cgtgtaatca gagccgcaag gcggcgctcc cactcctgcc gtccctgccg gatctcagat
     9001 aagttttcca tggtcatctg ctgaccatta aaggtgacgg attttccgtc cagcaccgcc
     9061 atttcagctt ccgtataacg ctgaatcatg gcttcgatat cattctggtt cataaccatc
     9121 ctccggaagt cagccagggg ttaacatcgt cagttactgt tttcttccgt ttttgttttt
     9181 taacaggcgt ggataccggt tccggtgagg gtgacggttc ggtactgtcc gggacactct
     9241 ccagccaggt ttcccggctc gcccaccccg gtgcatccgg ccagcggatc ttttcgtatc
     9301 catgcagaat gaccagagcc tcggcataca ccatcaggtc aaaagcttcg ttggcaccgc
     9361 gacccggctt actccatttc ccgtcactac tccgctcttc atacgtcagt tcgtcgtaaa
     9421 accagctccc cagccagtca gggaaatgca catagccggg acctggagag tcacgccata
     9481 acgcgttatt cacccggtct ttcagtgcat ccgtctgaag aagccagagc ggcacatcac
     9541 ctgcggcctg cgcccgtcgg cccgttcgtc cggtgttatc agggaatgta cgggtgatca
     9601 gttttgcgcg ccggatgctg tcgcccttaa acaggtaaat acgtttacca aggccatcac
     9661 gacggcaacg acgccagaat ttataggcat tatcagtgac cccgtcttca ccgccggagt
     9721 ccaccgccat tgccatcagt cgcatttgtt gagaaggatc ggaggccagc ggccagcttt
     9781 tatgaaaaac atccgtcagc aggacatccc agtcttccgg atagctggcc ggatcaattc
     9841 gctggctctc accgtcgctg tcaccgcgca atgactgcgt gatgttgtaa cgatcaataa
     9901 tccagcgttc gccacggctg ccatagcccg ttacctgaac cacaaaacgg cgatgacgtc
     9961 ccgcctgcac atccactgtc gccacaagga aattaacgcc atccggcaca ctgctggaag
    10021 gaactggctc tgcccgctgc tcaagcagtt cactttttcg ttgctccatg ctggcgcggg
    10081 gaagataagg taatccccag tcggtattga taaccgtctt gagtgtttct tcacttccgg
    10141 ttgtctcgta ttcctgttct gcagtaagca gtttgtaaac gagttgcgag agtgtctggt
    10201 aagcagctgc cggaccctcc atccagaatg acgcaatacg tgagcgtcgg ggatcaccat
    10261 aacgactacc atccgcattg atggattcac catcccgcaa ccagacccca cgtccgttca
    10321 gctcacgttt ttgttcaggc ataatccgtc ctgaacagga aggacactga atataagccg
    10381 cctcacttgc cagcacggga tcggcaatat cacggaaacc agcaaccaca tcgccgcagg
    10441 gctgaaaata ctcaccacag tgtggacagg gccagtacca gcgacggcga tcgccacggt
    10501 tatagagcga cagtatcccc gtggttggtg gagcctcatg cggtgaagtc cgtcgccatt
    10561 tcacatcctt cacatccctg ccgggggaac tctccaccag cgtcatacca ctggacataa
    10621 atgtggtggt acgttttgag gcaagagaga aggcatcccc ctcgccatca atatcttccg
    10681 gaaaacggtc ataatccgtc agcgccacgc atttataatc tgatgaggac atgatattga
    10741 ctgacggcca gccgattttc aggtagttgc cagcaaggaa tgttctgtca taaacgttgt
    10801 tgtcattttt gttcggactc aggcgactga ccacttccgg gctgacgcga aacgttctgg
    10861 cgagtcgttt tttggagtgt tcgcgggctt tttcctccgt catctgaatg atcagcatat
    10921 ccgcaggatc gcaaatcacg ttgtaaatca cccagccgtc aatcaggccg atagtcttgc
    10981 cggttcgtgc cgggccaaca aatatcactg cgtcgtattc acgcgaggcc aggcagttca
    11041 tcggctcaat aacatacggt gccaccagcg gatcccacgg gactgagttc ccggccccca
    11101 tgggcacccg catatactga gcaacggcat cagcaacccg cattcgtctc ggtgcgcgaa
    11161 ggatataacc tgaatcggtt cgtgctgcct ttgcggtttc ctgattcagc attactcctc
    11221 ctgctgtaat tcctcctcat catccgcacc tgcttcggtc acccgcaggg ctatctgatc
    11281 gcgcagatca tcaataatgg actgaacacg gctcacagcg gcaggctgca gaccacagtc
    11341 acgttccaga atatccggta atgtctccag cacctgcacg accgcttttg cccagatggc
    11401 aaactcccgt ctgacatcac tggccggaat gagttgtgcc gtttcctgtt cgaacttaag
    11461 acgctcacgt tcagactgat accaggcttt gcgttcatgt ggatccattt cgccctcagc
    11521 aaccggcggt ggtaacccca taaattcagt cagaatatcg gtcaaccggt atagcttgag
    11581 tttgtcatgt ccaccagcgg gacgaatgtt tttcagtctt gccacgacag tctggcggtg
    11641 cagaccagat aaagccgcca gttgattaat attcagcacc aggtttttca actcatgatc
    11701 catatttcct ccggagagct ttaaacatgc atcgtgcgaa caactttaag aaaacgcgtt
    11761 cgatgtcgaa caaaaaacac tcaattcgac atacaaaaaa caaataacca ttaataatca
    11821 ataagatgca aagatgatgg tggccgataa aaatgcaaaa actagccttt ttccgcgacg
    11881 ctcccgcccc gtggcaggcc accccaccgg gaggagccgt cagcctgaaa gccatgacga
    11941 acgtctgata cagcccttgc atgaatggca tcgggataat ccagaaagga atagcatcga
    12001 cccacaagaa tctgtgtgag tgtcctgttt cttccacccc cgcacaggac tggcgagcat
    12061 gagggacaaa cccgcgaatc attagcgcgg taaaaacccg gtgtgcatcg tttttgatta
    12121 ttcccgcaca ctcgcgcaga aggagttccc cgtcgggcta cggtcatggt taatgcggga
    12181 atacagcgac gatacagcgc atgatgtgtc aggcttgaat acctttatcc gttaaaaggg
    12241 atatcagtta agttatcccg tgtagggtat aagccattat caaagccact ctgtagggag
    12301 tggcttttgt aatggcaata aaaaagcccc gcgaatgcga ggctaaatcc aggtattttt
    12361 aatgactggc tcttatctca acgcagcccc ttaccgcgcg ccagatgctc aatatcaagc
    12421 atcagcaatg agatgtttaa ccatgattca ctccagaagt gagcaccacc ctgtctacag
    12481 agccagatgt gaaggatgat gagtaaaatt atcgctatca tcgaaggcat tgcgtcctga
    12541 tgtattcctg aagcgttctc agtgctgttt ggtcgcggat aattccgtcc cggataccga
    12601 gaacgtttcg tccagcaact ggagagagtt cgacggtggc atcattgccc atgccggagg
    12661 cgctggaggt ttcgtctgag gatggcacag ggcattttcc tttgacgaac acccgaccac
    12721 cattatcaag cttgcgccga agagcatcat ttttagcttt cgcatcagct aactccttcg
    12781 tgtatttagc atcgagtgca tcagcagcac gctggcgctg ctgcatgtca gtaatggtgg
    12841 cggtcgcctg cttcagctca ctgacttttt tatcacgctg ttctttatag gcgatggcgt
    12901 tatcacggta atgattaacc gcccatgact ggcagacgat gatgcagata accagagcgg
    12961 agataatcgc ggttactctg ctcattgttg cccccacaaa cagacttcac gctcaatctc
    13021 gcggcgagtc atcagccctt tccattgctt accgccagcg tatgtccagc gccgtagctg
    13081 atcacatgcg cctttgatat caccctggtt tattttgcga agaagcgtcg atgttctgaa
    13141 attaccagcg cccacgttgt aaacgaacga gtaaagagcg ccgcgcgttg tttccggtat
    13201 atcgactttg atgtacgggt taatttgtct ggcgaccgtg gcaaggtctt tattcaggag
    13261 ggctttgcat tctgcttcgg tatacgtttt accgggaatg atgtcttttc cggtgtgtcc
    13321 gtgacataca gtccatacgc caacgatatc ttcgtatggt atgtagctga caccttccag
    13381 gccatcgtca ccactcggac cagtgatgag cacagacgct atggcagcag ccccaccacc
    13441 aatagcagca gcaacagcct tgcgtaatga tggcgacatt attcacctct cgcagcctta
    13501 cgcttatctt ctttaatctt gaaataaagg tttgtcagat acgtcagcaa gccaaacacc
    13561 aagctaccca atacgcctat tgccacccac tgggatgggg agactttgtc cagcaactgc
    13621 agtagccagt atcccgtccc caccgctgac gtggtgtatg acacacctgt tgttattttt
    13681 tccatctggt acataccccg tctcccgtta tccggaagct gacaacaata aaaagccacc
    13741 agttaattcc tgatggccct gatgcataaa cgtcataata cctgactgtt atgattgaca
    13801 ataatgataa tgtttatata gaaaggttcc cgatgtgtgt tacatatcat ttctccacgg
    13861 ggaatatccc cacgccagcg cagactcttt tacccgttct cttctgcgct ggctcttttt
    13921 tattatgctg ctgcatttac ctctggcacc atgctttcta tctcaacaca atacgtggta
    13981 cttcttgtaa ccaatatcat aacgattaat cgacatagaa tttctcccgt gtacaggaac
    14041 agagttaaaa agccggaacc ggaatcaaat cacaggatga ccatctgcca gtggcaggtc
    14101 ataaaaaaaa ggccgcgcca tgcgcagcca gaactcacaa ggaaaatgat agaaggaaat
    14161 aacattagtg atgtacgcat ggcgcctccc gctaagttct gcaatgatca aacagaactc
    14221 gctacgtgcc cttaaaactc gatcatttag cccctccaag gaggattcac catgcggttg
    14281 attttttaat aaacagtaaa caaaaaagtc aagaattatt cattctgttc tttcatcatc
    14341 ggccacagca ataccacaat gccgcagacc agagcgccat cagtcagtac caacattatc
    14401 ctgctggtga aatccatcat caccatcact aaaagcagga tcacaacagc aagcagacac
    14461 agtttataaa acaatgttca ggaaacgcat tcagcatgcc taaggttcta ttcctacgaa
    14521 tagccaactt gcaactt
//
LOCUS       DAZKIP010000107        14360 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZKIP010000107 DAZKIP010000000
VERSION     DAZKIP010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.108"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            190..883
                     /locus_tag="ACHOUK_003580"
                     /pseudo
     CDS             190..883
                     /locus_tag="ACHOUK_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1107..1403
                     /locus_tag="ACHOUK_003581"
     CDS             1107..1403
                     /locus_tag="ACHOUK_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9898953.1"
                     /translation="MPDQSGYCWTPSRILRMPELSQLLGISRSTIYEKMNPLSKYYDA
                     TFPRPVRLGSGSVGWRSSAIDEWLTLHTVPARSAVKGVNDEVRHTLSSDMTMSR"
     gene            1711..1827
                     /locus_tag="ACHOUK_003582"
     CDS             1711..1827
                     /locus_tag="ACHOUK_003582"
                     /inference="COORDINATES: protein motif:HMM:NF024547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9898954.1"
                     /translation="MSIACQNQVDVCSPGNLRGAVNIYTMVLEKGKRPGERY"
     gene            2046..3479
                     /locus_tag="ACHOUK_003583"
     CDS             2046..3479
                     /locus_tag="ACHOUK_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFP9898955.1"
                     /translation="MCLLAPENPYPIYALPPLVRNAIIETQKNTQAPLAMVAISALTA
                     FSIACQNQIDVCRPGNLRGPVNLYSLILADSGERKTTVDKVFMKAFYLRDEALAEEYA
                     KLVENYSTEKEIWEQKQKALESKLYKEIRAGKDCKATESELKRHLNKCPVPPQIRRTI
                     FNETTIEGMLKYYSDSNRSFALVSSEGGIIFDGRAMSKLGILNSLWDGGSLFIDRKSS
                     PGIILKDPRLTVSVMIQPDVYQKGFCTRKKELVKTSGHHARFLMCQPTSTQGTRIITG
                     DNYSSQYQDLFEQRINELIDESLAMSGERRCLHFSPQAARIWTDYYNDVESKLGGLGP
                     LRHCREYAAKNAEYMARLAGLIHHSSGEEGDISPYTAEMARELAIWYGNEYVRLSNPL
                     TFDNPALTVPVRLIPEELELFNWIKSYCIEKGIPCMKKNDILQRGPNRFRKKDKINWL
                     LDLLYEQNRVVPVIEGKTLYVAPNFDL"
     gene            4435..4998
                     /locus_tag="ACHOUK_003584"
     CDS             4435..4998
                     /locus_tag="ACHOUK_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFP9898956.1"
                     /translation="MYNANPNYEMNFAILKDVNEHMEGLFQRFSKLLPFRIDFAYRKD
                     TPSFGYSCKHSMCIEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            5249..5415
                     /locus_tag="ACHOUK_003585"
                     /pseudo
     CDS             5249..5415
                     /locus_tag="ACHOUK_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(5575..8817)
                     /gene="zorD"
                     /locus_tag="ACHOUK_003586"
     CDS             complement(5575..8817)
                     /gene="zorD"
                     /locus_tag="ACHOUK_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001477932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I Zorya anti-phage system protein ZorD"
                     /protein_id="HFP9898957.1"
                     /translation="MLKRLLSKLTGNRQQIEHHLKNQYQVEENGLSFPLSLVDDSQLW
                     ALASWLEQLAEEDYLISLTDRWLLSWEALYRLLEDEEHASSLPLIGVPDILPLRASLS
                     SRGALSDSDFRVWIAEWATFPARKPIRFSRTGAILTHDNQQYLLSRENWALLQATEQL
                     SAQQIQTPGETTNQLGWAAIRKCAKLAAAKFDDYLEKTHVIKPTSLSLRLRKATVADT
                     AVIEIEPHFEDQPANWLGSFDKNLQVHDSYRIPGENGELSHVIIPPEVKEVLNSIHSI
                     PGRRVAGSEALSFVRNPYTFLGEDAASVIAPEEHEQALFDARIFFHHFRLIPQLNTEN
                     KITEVTLILEPVSPVPQPEITFVFSAPWELDKFIQQLGISVAAQMPAGSWQGYELELS
                     QFTEQQWHDCQALLTRWQQEIEGKEFSDVLDIAKYGDRVIGIGEFEKISSPWLTKAQS
                     ENWLPDGIDFSAFSIETLSGWQPENPLHFDELQEIITQAEAAGETYITTPWNDSQLPL
                     DAAKTFSKNWEKQQITANEYQGNVADKTARAVLKIEQNIEETAYIKQRRNSLLNARHA
                     EPEIPLSLKEHIRLKDHQREGVAWLQQLFLRSPEETAGCLLADDMGLGKTLQILSFLV
                     WFIEKFPQEPPNLIVAPVSLLDNWERELNNFFYTAGIPVLKLYGETIKAVKYPKQAIP
                     AHLQSKGIKNLLKPGWQGEAKIILTTYETLRDQEFSLARQPWSIMVCDEAQKIKNPAA
                     LITHAANAVQARFKVACTGTPVENTLVDLWSLFDFAQPGLLGALNEFGKQYVRPIENE
                     DGRDTERLESLRALIEPQTLRRTKEEVARDLPQKIEVESCKQLTLSGVQKQLYLSSVA
                     NWQQQQALREGMQQAGTGMLGLLHRLKLICAHPAIVNPEPRFRDNSPKLNWLLKILAE
                     IKHTSKDKVIIFTELRDLQRELQHAIHQNFGFRPVIINGDSSTKSQSQNSRQRLIDDF
                     QAQPGFGVIILSTVAVGFGVNVQKANHVIHFTRCWNPAKEDQATDRAYRIGQTKNVYV
                     YYPTVRDTEITTFEETLDDLLQRRRALARDMLCATPDLNCADFETILKGA"
     gene            complement(8817..10499)
                     /gene="zorC"
                     /locus_tag="ACHOUK_003587"
     CDS             complement(8817..10499)
                     /gene="zorC"
                     /locus_tag="ACHOUK_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I Zorya anti-phage system protein ZorC"
                     /protein_id="HFP9898958.1"
                     /translation="MIPALNSLSQRIAARLSSSQRDDHYLHNDFHALTAATLDMEKRF
                     DRAERIPSPPQEMRLEALRRFRLAQELTEREWRMVFYGLADNDPLYPDQPILLEDDTF
                     FPRVNSAIKKRIETKTLKRRDWAALCSSYFAYQHQSPETNPHWCVLRGHIAQGYLVVK
                     AAIRREKSWMKTIEFYHDIFTPQAGGVISRQLLAGENNSLSSLEKIAQIPDSSWLWKR
                     IFTVLLAQLDTLDDPQFLSKISWLLGLAAQWVRFRDDIMTATLTRYYHSGYRDQSHPA
                     LKQAALEYWDNPQLKSQQNKWHQYVSESVAAMVRGWLAKQDLTHFFELLRGNGDVDQA
                     RLHYWLRFANQMGFTRIIMGSDAWQDRGSDFVKFREENKGRLSYLRGGRNFDNAMVMQ
                     INDYLFVEFSGTGNAMYAYQIGHAPFNPESRTLDINIHLKDQGRCALRLPHAPRAEGY
                     NKVRITGWMLKYDDELRKLGIRWMAEEPVRFVDKKVPPPVAMSDIKIINPLRDTAIQH
                     LVKCSSCIVSDNRHKGGILSVQLITPDDTVEKELLRLGFAPVAKEPHRYWIK"
     gene            complement(10496..11236)
                     /gene="zorB1"
                     /locus_tag="ACHOUK_003588"
     CDS             complement(10496..11236)
                     /gene="zorB1"
                     /locus_tag="ACHOUK_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I Zorya anti-phage system protein ZorB1"
                     /protein_id="HFP9898959.1"
                     /translation="MFGNAFGVKKRRSDEAEKPFWISYADLMTAMMVLFLVVMVASLS
                     SVTQRIQRAEQGEKARGQDISRLCERLELHARNVNKNIVVDCHDNRISFGEAGRFAHN
                     QFFLNAEGQKALQDVVPLVLEASNSEEGKKWFKQIVIEGFTDTDGSYLYNLHLSLQRS
                     EWVMCSLLDSRSPLQKNISAEQQLQIRKLFLAGGVSFNNAKESKEASRRVELRMQFFG
                     LKDKRDKADEVDFPPVVNKEVCQLVMPL"
     gene            complement(11250..13439)
                     /gene="zorA1"
                     /locus_tag="ACHOUK_003589"
     CDS             complement(11250..13439)
                     /gene="zorA1"
                     /locus_tag="ACHOUK_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I Zorya anti-phage system protein ZorA1"
                     /protein_id="HFP9898960.1"
                     /translation="MSWLNSILVTLTSVEPYKVPVTVIVTVTFAFVCFIFFYLLRSIR
                     IIYGLKKYTRSINSIEKSAPEVQLEHLKSLFQRSELKHAWNEFEESLHSQYELENGEE
                     KIVRIRATAPSASFFSEQQLVDIPLNTEFFKHLPGILTGMGIIGTFYGLMIGLNHFDP
                     STPEQVSSSVNNLLRDVLYAFLGSAFAIFASILVTWLEKLSIAKSYKYLEKFTAALDS
                     LYDSGVGEEYLASLVKSSNESATQARHLKESLVTDLRDMLLHLAESQKIENERLANTL
                     SATYRESGSQFADQVSGAIENSLKSPLDKIAGAVQTASGDQSGMVQNMLQNVLTAFMA
                     KLDTTFGQQFTNLNEMMGQTVGAIQTMQTGFSALLQDMRQVSDDSRQGSAQLIEQLLS
                     EMKSGQQALQAGMNDMLTSLQVSVAKIGAEGEGAGERIARQLEKMFADSEAREKAQAE
                     HMAAFVEAIQNSVQQGQSATMEKMAASVGALGEQLGSLFGQIDKGQQQISATQQANQQ
                     SLHEQTQRVMSEVDDQIKQLVETVASQHQGTTETLRLLAEQTNRQIQDMQAGADKMRL
                     AAERFEHAGERVSEANHLTADVLNKAQSAGSSLSLATSELTSVVADYRNNREAVSKSI
                     AMLELLAANTQSEQTTRNQFIADLKQHGERLQSYNREAQVFMENVSDVLGKGFEDFSE
                     GVSRSLDKTLGKLDVEMAKASNLLAGSVEQLGESVSELDDVLSRVRT"
BASE COUNT         3750 a         3140 c         3322 g         4148 t
ORIGIN      
        1 tccttttatt gcactatata aaattttaac attatatcat tttgcttttc tgttaactgg
       61 ttttcatttt tgaatcaata ttttctgaat agggtaatag tacacgtatt aacagattat
      121 gtcagaaggc cccggtgatg ccttctgacg atagattaat gtggtattct attggtatct
      181 ggagaccata tggcaacagt tacagtacat tctccctgct gtaattccta taaagtgtac
      241 cattatgatc acagcacctc agatcatgag cgtttccagt gctaatcctg caaacgtgtg
      301 tatttcaact gaattacatc tacgaaaccc gaaaaccaga cattaaagag caaattgttg
      361 aaatggttca taacggagtc ggggttcgcg atagctccag aacgctgaag gtggatatca
      421 acaccttcat tctcacttta taaaacgctc accacgcaaa gtaaccgcat ctccagtcgc
      481 cagtgccgat gtgacgctta tctgcgaact tgatgaacaa tggagttttg tcgaaaacaa
      541 agctcgtcag cagtggcact ggtatgcgta taacaccaaa tctggaggtg tgctggctta
      601 cacttttggt cctcgcactg atgaaacatg tcgtgagttg ccggaactcc tgaagccatt
      661 ctccacaggc atgataacca gagacaaccg gagcagttat accagagaaa tgccacagga
      721 taagcatctt gtcggcaaga tatttacccg gcgcattgag cgcaacaatc tgacacttcg
      781 tactcacatc aaacgaccag cccggaaaac gatttgcttt tcacgctccc tggaaattca
      841 cgaaaagcca ttggttcatt tatcgaaaac aatgtactac tgactggagt cattaccagg
      901 gcctcttttg ctgttttttt accaatgaat gataatgaga atcgacagcg ataaggggaa
      961 agtaaaataa cactaatgct gtcgttttct atcatattga tttttaatta tttattgtct
     1021 ttttgctcag cccatttttt tacgcagaca aatttacccg gggaatacaa actgtaacga
     1081 cagttcacca cactatgggg gataaaatgc cagatcaatc cggatactgc tggactcctt
     1141 cacgcatctt acggatgcct gaactgagcc aattgttagg tatatctcgt tcgacaattt
     1201 acgaaaaaat gaatccgtta tcgaaatact acgatgcaac cttccccagg ccggtacgtc
     1261 ttggtagtgg ttctgtgggc tggcgttcat cagctattga cgaatggctt actttacata
     1321 ctgtacctgc cagatcagca gtcaaaggtg ttaatgacga agtgcgacat acgctttctt
     1381 cagatatgac catgagcaga taagtttttg tcatcacgtg atcacctcag gaggaaaaag
     1441 aagcataaga acaatttagc cagaagttta attgataaac agaatttcca gcattcattg
     1501 ttgaaagagc agcgattgtc gctattgtag cggtaattgc tgctataacc aaaacgtatt
     1561 acgaaaaagg aagcatttat gtcttacatc aaaccggata tgccgtattc agtatatgcg
     1621 tgaccatcaa tgatagggaa ggcaataatt aaagctcaat acaataaaca agtacctctg
     1681 actctggttg caatgtcagt attaacagcg atgtcaattg cctgtcagaa tcaggttgac
     1741 gtatgcagcc ccggaaattt acgtggggcc gttaatattt acactatggt tctggagaaa
     1801 gggaaaagac cgggggaaag gtattagtga aataatttct ggaggtatta agaatgagaa
     1861 atatgaatga tatttcgtgg taaataatac agtctgcact gaattaacct tatcggttta
     1921 ttaaacatca gatctacaaa tgctctgtat ttctcagagc atcaggtctg aatagcagct
     1981 attttcgcta ttgtagcggg agttgctgct attttactaa cccattaatg aaaagagagg
     2041 cttttatgtg cttacttgca ccagaaaatc cttatccgat atatgcacta ccaccgctgg
     2101 tgagaaatgc aataattgaa actcaaaaaa atacacaggc acctttggct atggtggcga
     2161 tatccgcatt aacagcgttc tcaattgcct gtcagaatca gattgacgtg tgcagacctg
     2221 gaaatttacg tgggcctgtt aatctttact ctctgattct ggctgattcc ggtgaaagga
     2281 aaacgaccgt ggataaagtg tttatgaaag cattttatct cagggatgaa gccctggcgg
     2341 aagaatacgc gaaactggtt gagaattaca gtacagaaaa ggaaatatgg gagcaaaagc
     2401 aaaaagcact ggagtcaaaa ttgtataaag aaattcgcgc cggtaaagat tgtaaggcaa
     2461 cagagtcaga gcttaaaaga catctgaata aatgtcctgt tccgccgcag atacgccgga
     2521 caattttcaa tgagacaacg atagagggaa tgttaaaata ttattccgac agcaatcgct
     2581 cttttgctct tgtatccagt gaaggaggaa taatttttga tggccgggca atgagtaaac
     2641 ttggaatact taactctttg tgggatggtg gttctctttt catcgacagg aaatcatctc
     2701 ccggaattat tttgaaggat ccaagactga cggtatcggt gatgattcag cctgatgttt
     2761 accagaaagg tttttgtacg cgaaaaaaag aacttgtgaa aacgtcagga catcatgcaa
     2821 ggtttttgat gtgtcaacca acatcaacgc aggggacaag gataataacc ggagataatt
     2881 attcatcaca gtatcaggat ttgtttgaac agaggatcaa tgagcttatt gatgaaagtc
     2941 tggcgatgag tggtgaacga cgttgccttc acttttctcc tcaggctgcc agaatctgga
     3001 cggattatta caacgatgtg gaatcgaagc tggggggatt ggggccttta agacattgcc
     3061 gggaatatgc tgctaaaaat gcagagtata tggcaagact ggcaggactt attcaccatt
     3121 cgagcggtga agagggggac atttcccctt acactgcaga aatggcgaga gaattagcaa
     3181 tatggtacgg taatgagtat gtgcggttgt ctaatccgtt aacttttgac aaccctgccc
     3241 tgaccgtacc tgtgcgactt attccagagg agcttgaact tttcaactgg ataaaaagct
     3301 attgcattga gaaggggatt ccctgtatga aaaaaaatga cattttacag cgtggtccga
     3361 accgtttccg gaaaaaggat aaaatcaact ggttactgga tttactgtat gaacaaaaca
     3421 gagttgtacc ggttattgag ggaaaaacgt tgtatgtcgc acctaacttt gacctctgaa
     3481 cgacttaatc gttgagtaac atatagtctc ctctgctact tgcggaggag actaatgcct
     3541 ttaccatgtc agcgttgttg tattagaaaa ctctggtaca ggtcagtcag taaatataac
     3601 tggtgtaacc actatttact attggataga gtgttgctca ggatattgtc ggctttagtc
     3661 tgaggtgagg tgggaccttc attgttttcg tgttcaataa ataatttagg ttaattagta
     3721 ttattaatgg gagttatgtt atctgttatg ttgtcttatc tctttacatg gtaatatcac
     3781 tcctctttat gctaagtatt ctgtatgtgc gtaatacata tatctccata cactacgcag
     3841 tgaagcatat ggggggaagc tgtatggtga attatgtggt gtaatgttgg gaacataaat
     3901 ctgttatgtt gtcttgtacg cacttaacac tggttacgta ccggaacact attagagtat
     3961 attgtcttta gttcgtacgt tatagctccg ttacagttta tgggtaaata taatcttttc
     4021 tgtatgaaat aatcttctta ttgtgaggtt aaatatctgt aggctctctc tgcagagtct
     4081 ctgtttttac ttaatggtac ggcgatacta atagcgatgt tgcctgtggc tattcaatgc
     4141 tgacttttgt gaagatcaga ttatttcctg taagtaattg aatggtgctt tcttaaactc
     4201 aattatatga ggtgtgtttt ttattgtaat tcgttctggt ccttagttta agagagtctt
     4261 actgtattct gaatcattac cctattactg taatgaggta atacgaaaca gttttatcag
     4321 gttgtctgtg ctgctggtta cctcgtttat taatgccgcg gtacggcgtt tctgtactct
     4381 cagtatttat gtaattccac tttcctgtca cttttataaa gtaaggacta taaaatgtat
     4441 aatgctaatc ccaattacga aatgaatttc gcgattctga aagatgtgaa tgaacacatg
     4501 gaaggtctgt ttcaacgttt ttctaaactg ttacctttca gaattgattt tgcctacagg
     4561 aaagacacgc ccagtttcgg gtattcctgt aagcacagta tgtgtattga aatataccgt
     4621 ttacttagcg aaacccagac gatgttagca ggatattact gggtaatgga atatacacca
     4681 gacaaagggc ttcatattca ttttatcggc tatctggatg ggcaacgtca caagaactct
     4741 tatcagatat ccaggcaact gggggatatc tggaggcgga tcacggaagg ggagggttat
     4801 tttcacctgt gccgggctaa agacaaatac ccggtgcgta tcgatcatgt aattcactat
     4861 agtgataaat ctgcggtaga cgacttacgt tacgccctta gctatctggc gaaacaggat
     4921 cagaaagaac acggtattat tctgggccgt agcaggcttc ccgaaaaaag taaccggggt
     4981 cgaccacgac ataactgaat tacagctgta ttagaattaa gtttctgtaa gttgttggga
     5041 ggtgcttgag ttaataggca gcagagggcg gctttgtcat cagtgtaggg gactatgaca
     5101 ctttgaacgt catcttgggt gtagtgactt ttaacgtcag gtgatgcata agggcctttc
     5161 tctgagagac cctgtttaca caattaaata aatcgaaccc gaccaacaat tttgatgtta
     5221 acagcaggat tattgagggg caatcaatgt gttcttcggt agcgattacg gtagtgaacg
     5281 gcgctctact gtacgggctg agcgggacct gctgtctgaa tggtatctgc gccatatact
     5341 gagagttttg ccggaatggt cttccaacct gttgatgaac tcttgccatg gaacgtagta
     5401 ctcaccaata aataaccgtt aatacagcgt tcggataata atactaacga taatagtact
     5461 cctctgggca tttttaattt atgtttttcc gaagctatcg gtaggtaata gcgaatcatc
     5521 acagctaagt gttctttcag gtttggcaac tgatagtatt gccgctcata tcggttaggc
     5581 tcccttcaaa atggtctcaa aatccgcaca gttcaaatcg ggagtagcgc agagcatatc
     5641 tctggccaat gctcgtcggc gttgtaagag atcatccagt gtttcttcaa atgttgtgat
     5701 ttcagtatct ctcacagttg ggtaatagac atacacattt ttagtttgcc caatgcgata
     5761 cgcccgatcc gtagcctgat cttctttggc tggattccag cagcgagtaa aatgaataac
     5821 atgattggct ttctggacgt taacgccaaa cccgacagca accgtagaga gaataatcac
     5881 cccaaaccca ggctgagcct gaaaatcatc aatcaggcgt tggcggctgt tttggctttg
     5941 acttttagtg ctgctatcac cattgataat gacagggcgg aaaccaaaat tttggtggat
     6001 tgcatgttga agctcgcgct gtaaatctcg aagctccgta aaaataatga ccttatcttt
     6061 cgaggtatgt tttatttctg cgagtatttt cagcagccag ttaagcttgg gtgagttatc
     6121 acgaaaacgt ggctctggat taactattgc agggtgtgca cagatgagtt tcagtcggtg
     6181 taacaaacct aacataccgg ttccggcctg ctgcatccct tcgcgcagtg cttgctgttg
     6241 ttgccaattt gcaacagacg aaaggtagag ctgtttttgc acgccagaca acgtcagttg
     6301 cttgcagctt tctacttcaa ttttctgagg taaatcacgt gcaacctctt ctttggttcg
     6361 gcgtaaagtc tgaggttcta tcagcgcccg caggctttcc agccgttctg tatcgcggcc
     6421 atcttcgttc tcgatggggc gaacatactg tttaccaaat tcatttagtg cccctaacaa
     6481 tccaggctgg gcaaaatcaa ataagctcca cagatcgaca agtgtgtttt cgacaggcgt
     6541 tcctgtgcat gccactttaa acctggcttg taccgcgttg gccgcatgtg tgattaacgc
     6601 agctgggttt tttatttttt gtgcctcgtc gcacaccata atggaccatg gctggcgtgc
     6661 cagagaaaac tcctggtcgc gaagcgtttc gtaggtcgtc aggatgattt ttgcctcacc
     6721 ttgccagcca ggtttaagca ggtttttgat ccccttagac tgcagatgag cagggatggc
     6781 ctgtttggga tatttcaccg ctttaatggt ctcgccatac aatttcaata ctggtattcc
     6841 tgcggtatag aagaaattgt tcagctcacg ttcccagtta tctaaaagag agacaggagc
     6901 aacaatgaga tttggcggtt cttgcgggaa tttttcaatg aaccatacca ggaagctcag
     6961 aatttgcagc gtttttccaa gccccatatc atccgccagc aggcagcctg ctgtttcctc
     7021 aggtgaacga aggaagagtt gctggagcca ggctacgcct tcacgttgat ggtctttaag
     7081 tcggatatgc tctttaaggc tcagaggaat ttctggttcg gcatgacgcg cattgaggag
     7141 tgaattgcgg cgctgtttga tgtaggccgt ttcttcaata ttctgctcaa ttttgagtac
     7201 ggctcgggcc gttttatctg caacgttgcc ttgatattca ttagcagtga tttgctgttt
     7261 ttcccagttc ttactgaagg ttttagcggc atccaacggt aattggctgt cattccatgg
     7321 tgtggtgata tacgtttcac cagcggcttc tgcctgagta attatctcct gtagttcatc
     7381 aaaatggaga ggattttcag gttgccagcc agatagtgtt tcgattgaaa acgcggagaa
     7441 atcgatgcca tcaggaagcc agttttcact ctgcgctttg gtcagccagg gagaggatat
     7501 tttttcaaat tcaccaatgc caatcactct atctccatac ttcgcaatat ccagcacatc
     7561 gctaaattct tttccttcaa tttcttgttg ccagcgagtt agcagtgcct ggcaatcgtg
     7621 ccactgctgt tcggtgaact gacttaattc cagctcgtaa ccttgccatg aaccggcagg
     7681 catctgtgca gcgacgctaa taccgagttg ctgtatgaat ttatccagct cccagggcgc
     7741 tgagaacaca aaagtgattt ctggctgggg aacaggtgaa acaggctcaa gtatcagcgt
     7801 aacttctgtg atcttatttt ccgtattcag ttgcggtatc aacctgaaat gatgaaagaa
     7861 aatcctggca tcaaacagtg cctgttcatg ttcttcaggg gcaataacac tggcggcatc
     7921 ctcacccagg aaggtataag gattgcgaac aaaggaaagc gcttcacttc cggccactcg
     7981 acgccctggg attgagtgta ttgaattcag gacttctttc acttcgggag ggatgatgac
     8041 gtgactgagt tcgccattct ctcctgggat gcggtagcta tcgtgaacct gtaagttctt
     8101 gtcaaaactg cccagccagt tagcaggctg gtcctcgaaa tgaggttcaa tttcgataac
     8161 tgcggtatcg gcaactgttg ctttacgcag acgtaatgac aatgaagtcg gtttgatgac
     8221 gtgcgttttt tctaaataat catcaaattt ggcggcggcg agtttcgcac atttgcggat
     8281 agcggcccat cccagctggt tagtggtttc gccgggagtc tgaatttgct gtgcactgag
     8341 ttgttctgtt gcctgtaaga gcgcccagtt ttcccgcgat aacaggtatt gctgattatc
     8401 atgcgttaaa atggcaccgg tacgactgaa acgaatcggc ttgcgggctg gaaatgtagc
     8461 ccattcagca atccagacgc gaaaatcgct atcgcttaat gctcctcgtg aactcaggct
     8521 tgcgcgtaag ggcaatatgt caggtacacc gataagaggc aggctgctgg catgttcctc
     8581 atcctccagc aggcgatata gtgcctccca actcaataac caccgatctg taagtgatat
     8641 cagatagtct tcttcagcaa gttgttcaag ccaggatgcc agtgcccaaa gctgtgaatc
     8701 atcaaccagc gatagcggaa agctgagacc gttttcctca acctgatact gattctttag
     8761 gtggtgttct atctgctgac ggtttcccgt cagtttactt aacagacgtt tcagcattat
     8821 ttgatccagt aacgatgcgg ttctttagct acaggtgcaa agcctagtcg taacaactct
     8881 ttttctacgg tgtcgtcagg tgtaatgagt tgtacggaca gtatgccacc tttatgtcga
     8941 ttatcgctga cgatacatga actacattta accaggtgtt gtatagccgt atcacgcaac
     9001 gggttgatga ttttgatgtc agacatagcg acgggagggg gcactttctt atcgacaaac
     9061 ctgacgggtt cttcagccat ccaccgaatg cccaacttac gtagctcatc gtcgtatttc
     9121 aacatccagc cggttatccg gactttgtta tacccctcag cacgaggtgc atgggggagt
     9181 ctgagtgcgc atcgcccctg atcttttaga tgaatgttga tatcaagcgt gcgtgattca
     9241 gggttaaatg gcgcgtgacc tatttgatag gcatacatcg catttcctgt cccggaaaac
     9301 tcgacaaaca gataatcgtt gatttgcata accatggcgt tatcaaagtt acgcccacct
     9361 cgtaagtaac tcaatcgtcc cttattctcc tcgcggaatt tcacaaaatc gctaccacga
     9421 tcttgccagg catcggaccc catgatgata cgagtaaatc ccatctgatt agcgaaacgc
     9481 agccagtagt gcagtcgagc ctgatcgaca tcgccattac cgcgcagcag ttcaaagaaa
     9541 tgtgtcaggt cctgttttgc caaccagccc cggaccattg cggcaaccga ctcggagaca
     9601 tactgatgcc atttattttg ctggcttttc aactgggggt tatcccagta ttccagcgcg
     9661 gcctgcttca acgctggatg tgactgatcg cgatagccag aatggtaata gcgagtcagg
     9721 gttgcagtca tgatgtcatc acggaaacgc acccactgcg cagctaagcc taacaaccag
     9781 ctaattttgc tcagaaactg gggatcgtcg agcgtgtcca gttgagctaa aagcacagtg
     9841 aaaatgcgct tccataacca actgctatca gggatttgcg cgattttctc taatgatgag
     9901 agtgaattgt tctcacctgc aagtaactgc ctggagatca ctcctcccgc ttgtggggta
     9961 aaaatgtcgt gataaaactc aatggttttc atccacgatt tttctcgtcg tatagcggct
    10021 ttcactacca ggtaaccctg agcgatatgc ccccgcagta cgcaccagtg agggtttgtc
    10081 tcgggtgatt ggtgttggta ggcaaaatag cttgaacaaa gtgctgccca atcccggcgt
    10141 ttcaacgttt tggtttcaat acgttttttg attgcactgt ttactctggg gaaaaatgta
    10201 tcatcctcca gcaggatcgg ctggtcagga tataatggat cattatcggc gaggccataa
    10261 aacaccatcc gccactctcg ttccgtgagt tcttgtgcca ggcgaaagcg acgtagagcc
    10321 tccagccgca tttcttgtgg cggtgagggg attctttctg ccctatcaaa ccgcttttcc
    10381 atgtcaagag tagccgcggt caacgcgtgg aaatcattat gcagataatg gtcatccctt
    10441 tggctggaag agagcctcgc tgcaatgcgt tgagaaagag aattcagggc gggtatcata
    10501 gtggcataac caactggcaa acttctttat taacgacggg gggaaaatcc acctcatcgg
    10561 ccttatcacg tttatcttta agcccgaaga actgcatgcg caactcgacc cggcggctgg
    10621 cttccttgct ttccttagca ttattgaagg agacgccacc cgcgaggaaa agcttacgga
    10681 tttggagctg ctgttctgcg gagatatttt tttgcagagg gctgcggcta tcgagcagac
    10741 tacacattac ccattcagag cgttgcaggg agagatgtag gttgtataag tacgaaccgt
    10801 cggtgtcggt aaacccttca ataacgatct gcttaaacca cttcttaccc tcttcactgt
    10861 tggacgcttc cagtacaaga ggaactacat cctgcaacgc tttttgcccc tcggcgttca
    10921 ggaaaaattg gttatgagcg aaacgcccgg cttcgccaaa gctgatacgg ttatcgtggc
    10981 aatccactac gatgtttttg ttcacattgc gggcatgcag ttccaggcgt tcacacaatc
    11041 tggaaatatc ctgccccctt gctttttcgc cttgctccgc acgttgaata cgctgtgtaa
    11101 cggaactcag tgacgcaacc atgacgacca gaaataacac catcattgca gtcatcagat
    11161 cggcatagga gatccagaat ggtttttctg cttcatcgct gcggcgtttt ttaacgccaa
    11221 atgcgtttcc aaacatcagc catcacccct taggttcgta cgcgtgacag aacatcatca
    11281 agttcgctga cgctttctcc gagctgttcg acagatcccg cgagcagatt agaggctttc
    11341 gccatttcga cgtccagttt acccagcgtt ttatccaggc tgcgtgaaac accttcagag
    11401 aagtcttcaa atcctttccc gagcacgtca cttacatttt ccataaagac ctgtgcttcc
    11461 cggttatagc tctgtagacg ctcaccgtgt tgcttaagat ctgcgatgaa ttggttgcgc
    11521 gtggtttgtt ctgactgcgt atttgcggcg agcaactcca gcatggcaat ggatttgcta
    11581 accgcttccc gattgttacg ataatcagcc acaacggagg tgagctcgct ggtggcaagg
    11641 gaaagtgatg aacctgccga ttgcgcttta ttcagcacat cagcggtaag gtggtttgct
    11701 tccgacaccc gctcaccggc atgttcaaag cgttcagcag ccagacgcat cttgtccgcg
    11761 ccagcttgca tatcctgaat ctgccggtta gtttgttctg cgagcaaacg aagagtttct
    11821 gttgttccct gatgctggct ggcaacagtt tcgaccaatt gtttaatctg atcgtctacc
    11881 tcactcatta cccgttgagt ctgttcgtgc agggactgct gattcgcttg ctgagtcgct
    11941 gaaatttgtt gctgaccctt atcgatctgt ccaaacaggc tacccagttg ttcaccaagc
    12001 gccccgacag aggctgccat tttttccatt gtggcgcttt gtccctgttg tactgaattc
    12061 tgaatggctt caacaaaagc agccatatgt tctgcctggg ctttttctcg cgcctcgcta
    12121 tcagcgaaca ttttttccag ttggcgagct atacgttcac cggccccttc accttctgcg
    12181 ccaattttcg ccactgagac ttgaaggctg gtgagcatat cattcatgcc agcctgtaga
    12241 gcttgctggc cagatttcat ctctgataac agttgctcga taagctgcgc gctgccctgg
    12301 cgggaatcgt cgctcacctg acgcatgtcc tgtaataacg cggagaaacc ggtttgcatg
    12361 gtctgaattg caccaacggt ttgccccatc atttcgttga ggtttgtaaa ctgctgacca
    12421 aaggtggtgt cgagtttcgc cataaacgcg gtcagtacat tctgcagcat gttttgtacc
    12481 atgccgcttt gatcgccgct ggcggtctga accgcaccgg caattttatc caatggtgac
    12541 ttcaagctgt tttcaatcgc accgctgacc tggtcggcaa attgtgaacc tgattcgcgg
    12601 taagttgcgc ttaacgtatt tgcgaggcgt tcattttcga ttttctgact ttcggccaga
    12661 tgtagtaaca tatcgcgtaa atctgtaacc aggctctctt tcagatggcg tgcctgggtc
    12721 gcgctttcat tactggactt caccagtgag gcaaggtact cctcgccaac gccgctgtca
    12781 tacagggagt cgagggctgc ggtgaatttt tccagatatt tgtagctttt agcgatggat
    12841 aatttttcaa gccaggtgac cagaatggaa gcaaaaatag caaatgcaga acccaggaag
    12901 gcgtacagta catcgcgtag tagattattg acgctactgg agacctgctc tggtgtgctg
    12961 gggtcgaagt ggttgagacc aatcatcaga ccgtagaagg taccgataat tcccataccg
    13021 gtcagaatac cgggcaggtg tttgaaaaat tcagtgttca gcggaatatc gaccagttgt
    13081 tgctccgaga agaagctggc gctcggcgca gtagcccgaa tacggacgat tttctcttcg
    13141 ccgttctcca gttcatattg tgagtgcaac gattcttcga actcattcca cgcatgcttg
    13201 agttcagacc gctgaaacag gctcttaagg tgttcaagct gaacctctgg cgcacttttt
    13261 tcaatgctgt taattgaacg cgtgtatttt tttaatccat aaataattct gatggatctc
    13321 agtaaataaa agaaaataaa gcagacaaag gcaaaggtaa cagtgacgat aacagtgaca
    13381 ggtaccttat agggttctac agatgtaagt gttactaaaa tggaattaag ccaggacatt
    13441 gttttactcg tttaatatat tatccatact aaaattagtc atcagcaaca tagtcaacat
    13501 aattgattat gttgcattgg ttgtcactat gtcgtgcggt tacacctgaa tactcttact
    13561 gttcaataaa tgaacgatag aaacagaata atcaggtgag acattttctt agtttcctct
    13621 tttttaccat gcaaaagcaa atttcttgat gttgtgtcgt ggtcagcgtc ccctggacaa
    13681 tattttacgt gccgcattct aaatctgaat ctgcgataag atatgattac tcttccgttg
    13741 tgatccatcc tgaggatttt catggacaat acctgataac gatttcttaa tgagtaggat
    13801 cacaaattat ggaatgctgc cggtaaagtg cgagcctgca tgaggctacc gactaaaagt
    13861 atgtagaaca gggattctct tctgaaatat gttttcagat gtcttctgag agtttcgcaa
    13921 tactgacggt tgataatgta ttcctgatcg taaacctgtc gatggaagtc ggggattggc
    13981 atcctgatac agtatgtcca gaacgaactt agctttgcat ggtatgatgg ttctgcatca
    14041 acaaagccta tacttgcata aaagtcctgt gccaggataa acatggcata tggctgtgta
    14101 cctaccgccc gtacaaggga ccttccgctg gccctgtgat gatgatattg catgggatct
    14161 cacttctgtt gagtgtcgct gcaggtatgt ggcgttgatc ggttgctgga gaaaggccat
    14221 ggcctgacaa aataaatata cagggaagca ttttctcctc cttcttctgg tgtgttttat
    14281 tacgctcctc ctttcatgtc cttcccatat cctgaaactc acttaaatgc tgttgaatcg
    14341 tcacgcattg caattttcaa
//
LOCUS       DAZKIP010000108        14356 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZKIP010000108 DAZKIP010000000
VERSION     DAZKIP010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.109"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            118..474
                     /locus_tag="ACHOUK_003590"
     CDS             118..474
                     /locus_tag="ACHOUK_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFP9898961.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYGNDPKFGSN"
     gene            complement(525..1421)
                     /gene="yhaJ"
                     /locus_tag="ACHOUK_003591"
     CDS             complement(525..1421)
                     /gene="yhaJ"
                     /locus_tag="ACHOUK_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFP9898962.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFNGK"
     gene            1526..2227
                     /locus_tag="ACHOUK_003592"
     CDS             1526..2227
                     /locus_tag="ACHOUK_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFP9898963.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQHLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRTYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            2250..2414
                     /gene="yhaL"
                     /locus_tag="ACHOUK_003593"
     CDS             2250..2414
                     /gene="yhaL"
                     /locus_tag="ACHOUK_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFP9898964.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2548..3858)
                     /gene="cyuA"
                     /locus_tag="ACHOUK_003594"
     CDS             complement(2548..3858)
                     /gene="cyuA"
                     /locus_tag="ACHOUK_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFP9898965.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHDG
                     VVFTQQACVAEGEQESPLSVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3886..5217)
                     /locus_tag="ACHOUK_003595"
     CDS             complement(3886..5217)
                     /locus_tag="ACHOUK_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9898966.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFIIVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGLVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(5492..6856)
                     /gene="tdcG"
                     /locus_tag="ACHOUK_003596"
     CDS             complement(5492..6856)
                     /gene="tdcG"
                     /locus_tag="ACHOUK_003596"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFP9898967.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKAQEELLSKTYYSVGGGFIVEEEHFGLS
                     HDVETPVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6928..7317)
                     /locus_tag="ACHOUK_003597"
     CDS             complement(6928..7317)
                     /locus_tag="ACHOUK_003597"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFP9898968.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7331..9625)
                     /gene="tdcE"
                     /locus_tag="ACHOUK_003598"
     CDS             complement(7331..9625)
                     /gene="tdcE"
                     /locus_tag="ACHOUK_003598"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFP9898969.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDKNGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9659..10867)
                     /gene="tdcD"
                     /locus_tag="ACHOUK_003599"
     CDS             complement(9659..10867)
                     /gene="tdcD"
                     /locus_tag="ACHOUK_003599"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFP9898970.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSLRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10893..12224)
                     /gene="tdcC"
                     /locus_tag="ACHOUK_003600"
     CDS             complement(10893..12224)
                     /gene="tdcC"
                     /locus_tag="ACHOUK_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFP9898971.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(12246..13235)
                     /gene="tdcB"
                     /locus_tag="ACHOUK_003601"
     CDS             complement(12246..13235)
                     /gene="tdcB"
                     /locus_tag="ACHOUK_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFP9898972.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(13334..14272)
                     /gene="tdcA"
                     /locus_tag="ACHOUK_003602"
     CDS             complement(13334..14272)
                     /gene="tdcA"
                     /locus_tag="ACHOUK_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFP9898973.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         3713 a         3848 c         3354 g         3441 t
ORIGIN      
        1 ccatgtattt atcttaaata tcaaaacctt tataacatcc gaaaaagaaa gatacgaata
       61 gcatttacac aatacccgga atagactttc gtcactttta attaaaggga tgtttttatg
      121 cagtggtatt tatccgtatt aaaaaattat gttggtttct ctggtcgcgc acgtcgtaaa
      181 gagtactgga tgtttactct gattaacgcc atcgtcggcg ctattatcaa tgtcattcaa
      241 ttgattttag gtctggagct tccatatctg tctatgcttt atttgctggc aaccttcctt
      301 cccgtccttg cgcttgctat ccgtcgctta cacgataccg accgttcagg tgcctgggcc
      361 ttgctctttt ttgttccgtt tattggctgg ctcgtgcttc tggttttttt ctgcacagaa
      421 ggtacttctg gcagcaatcg ttacggaaac gatccgaagt ttggttcaaa ttaatcttag
      481 aattggggcg atatttcgcc cctttttatt aacaataata aatattattt tccgttaaaa
      541 agtttgggaa tttcccgcag acaccaggat ttcgcttccc ccatactgtc acgacgccag
      601 gccataataa tatcgatctc gctggtcgat tccgggctga cgacacgcaa ccgcccttcc
      661 gcaatatctt tttcgaccat cggatacggc atcgtcgcca cgccaagccc cgccagtaat
      721 gcctgacgtt tatcttcaat cgtgctcacc gttaagcgcg gctgtttgtc cagcagctgt
      781 acagtcaaca ccgggcgctc acgagcggta tccgccaccg caattccacg atatttcaca
      841 cgcgtcactt cagataacgg ttccggctcc tgatgaatcg ggtgatcagg cgcggcaacg
      901 tagacgttca ttaacgtata gagcttgcgc gagttgatct ccgacgagga acgaaaatgc
      961 atatccggcg cgataacaat atccgcccgc ccctgctcca gccgttccca cgcccccgcc
     1021 agcacttctg tgatgattgc cagttgggta ttggcttttg ccgccagttt gtcgattaac
     1081 gggaaaaagg caggtgtcgg taccagcgct tcggtcacaa tggtgagatg cgtttcccaa
     1141 ccgcgcgcga gagcttccgc atcagtagtc agtttatctg cggcttccag caaaacgcgc
     1201 cctcgctcca acaacatcct cccgacattg gtgaatttgg tgcgatggcc cgagcggtca
     1261 aacagcacca catccagctc ttcttccagt ttttgcatgg tgtagctaag tgcggaaggc
     1321 acgcgtccca gctcatccgc tgccgccgca aaactgcccc ggcgatcgat cgcatccata
     1381 acccgtagtg cttccagcgt taatgccctt tctttggcca tttcgttctc attcaggaaa
     1441 tttgaacata ccgggcagaa tatctggcta acaatgtagc gtccagcccc ttaccataaa
     1501 aggaagtaaa gagaggtcaa gaattatgat tactacccga actgccaggc agtgtggaca
     1561 agcagactac ggatggttgc aggcccggta tactttttcc tttggacact acttcgaccc
     1621 gaaattgtta ggctatgcct ccctgcgtgt gcttaaccag gaagtgctgg ccccaggtgc
     1681 cgcctttcag ccgcgaacct atcccaaagt cgatatttta aatgtgattc tggatggaga
     1741 agcagagtat cgcgacagcg aaggcaatca tgttcaggcc agcgccggtg aggcgttgct
     1801 gctctctacc cagccgggtg tcagctacag cgaacacaat ctcagcaaag acaaaccgtt
     1861 aacacgaatg cagctttggc tggacgcctg cccgcagcga gagaatccgc tgattcaaaa
     1921 actggcgctt aatatgggca agcagcattt aatcgcctcg ccagagggtg cgatgggaag
     1981 cctgcaatta cgccagcaag tgtggctgca ccatatcgtg ctcgacaaag gcgaaagtgc
     2041 gaatttccag ttgcatgggc cacgcacgta tttgcaatcg attcacggga aatttcatgc
     2101 gcttacgcat catgaagaga aagcggcgct gacctgcggt gatggggcgt ttattcgtga
     2161 cgaggctaac attacgctgg ttgccgattc cccactgcgc gctttgctga tagatttgcc
     2221 tgtctagttg tttttacagg gagatgatga tgagtaaaaa atcggccaaa aagcgccagc
     2281 cagtgaagcc cgtggtggcg aaagaacctg ctcgcaccgc caaaaatttt ggctatgaag
     2341 agatgttgag cgagctggaa gccatcgtcg cggatgctga aacgcgttta gccgaggatg
     2401 aagctaccgc gtaaagacag gttgccggat gcggcgaaaa cgccttatcc ggcctacaaa
     2461 atccaacaac attaacgaat taaacaaccc gtaggccgga taagatgcgc cagcatcgca
     2521 tccggcacga tacccaaaac ctggcgttta tctggccttg ctcgccataa tctcgataat
     2581 ctgccgatcc gtttgctgca tcgaatggct tgctaacgca cacaggttgg caatcgactg
     2641 ctcaacatca tgcgccacga tcccttcatt gccggtcacg gcggtatcat ccagcgccat
     2701 taacaccgct ttccacgcag ccgaagcact ggtcgaaacc ttcatcgcgc agctgttcga
     2761 cgcaccatcg caaatcatgc cgctgacatc gccgatcata ctgctgatcg ccatcgagat
     2821 ggtttcataa cgcccatcca ccagccatgc catcccggcg gcggccccca ttgctgcggt
     2881 cgttgcggca cacagcgcag acaaacgcgg taactggtta tggatgtaaa ttgcgctcaa
     2941 atgagaaagc atcagcgcac gcgccagccg ttcatcatcc gctccgaagt gttctgctac
     3001 caccaccacg ggcattgttg cggtgatccc ctggttaccc gagccggagt tactcattgc
     3061 cggaagcgtg gcgccgccca tacgcgcatc ggatgccgcg ctggtacgaa tcacaatgga
     3121 tgaagagaga tctttcgcca gcaaaccgcg ctcgcactgt ttttccagcg tcgcgccaat
     3181 atgcagcccc cacttaccgc tcaaaccttc ctgcgataac gcacaattta gcttcgcgga
     3241 atcgagaata aagcggatcg ccgcaaacgg gacttcattg acgaacttca ggatctcagc
     3301 cagcgtcgtt ctggaaagca ccgatagcgg agactcttgc tcgccctctg ccacacacgc
     3361 ctgctgggta aacaccacac catcgtgcgt ctcaatatgc acaatgttgg tatgcccgcc
     3421 gacgatagtg acacacgccc acttctcacc gttccagact ttggcgcgtg agaagaggat
     3481 ttcattgcaa ggttcctgga tcttaacgga gactttcccc gccgccagca gtgctttggc
     3541 atcggcaatt gcctgcgcag ttgcgtcttt cagcacttcc agcccggcgt tagcatttcc
     3601 acctaacgcc cccagcgccg ccgcaatcgg cagccccacc attcccgtgc cgggaacggt
     3661 gacgcccaga ccgttcttca tcagatttgg cgaaacccag gcttctacac gttcaaccgg
     3721 accttccagt tctgccgcag caaccgccgc cgccagcgcc agtgaaatcg gttcagtaca
     3781 tcccagcgcc ggttttactt cctcctgaac ggcgaggatg taacgctgcc ataacggatt
     3841 taaagtcgaa tcaaacatat taaaaacctt aaaatttcag gtaaatcaag aaaatgccag
     3901 gaacggagaa acacaaagca acaaaccagt gacgataatc aggtacagag acatcccttt
     3961 gtatttgtgc aatgccggta ctttgtaaac cagccacgcc gggatcaggc accctaccaa
     4021 gccgaaaatc gggctacaga tagaggtgaa gctcaacacc ggtgcgttca gtacgatggc
     4081 gctccaggcc agcaaaatgg cgaaaatcat gatgccgcgc tgaacaagat tttcgttaat
     4141 cttctcggca ggcatcttgc gacgcaggat gttcattacg atcccttgcg ttgcttcgcg
     4201 aaaacctaaa tagacgccaa agaatgcagt catgacagca aagatattga gaatgacgct
     4261 gaccactttc acccacgctg cgccgtcacc gctaataaac tgtgcggcaa tcgccagcgc
     4321 ggaaatattc tgctcgtagg cttttaccgc ttcgtcatgt cccatcgcca gcgtgaacga
     4381 cacggcgtag aaaaagacga tgataaacaa aatgccaaac gcgatattca ttgcccgcaa
     4441 tgctttatgc cgcgctactt caatcgattt ttcccgcgag cgataagaga tcaccatcgg
     4501 gcttaacgtc tggataaaca gaatcgacgt cagggtaaac ggcagcgtaa taatagcgtt
     4561 tttcaccagc agccccagcg gcggtagcga accgacgtta tacagatgcc acattcccac
     4621 catcgacacg cccagcgccg cgaccaccag cagcttggtc agcaccatgc cagtcgaaat
     4681 tttgaataac aatttctcgc cgcgtgaaga gatcgccacc agaatgcaaa tcagtaccag
     4741 accataaaag ggactgtctg acagcaaccc ttccgtcacg ccgaaggtat gcaggtagga
     4801 agcactatcg ttggtgatgg cggtggaata gacgaacatc caaatcacca gcattacgaa
     4861 atagagtgca cctaacagga tgccccagtt tttacctaaa taaccgctaa tgacgctcgg
     4921 gtaatcttta cattctggtg attctgccag cgtattaata aacaaccgct gaaacagata
     4981 cattgccggg taaccaatca ccgatgagag caaaaatacc cacaatccca tcaaaccgac
     5041 ctgcaccggg agaaaaacaa tccccgcgcc aatcgccatc ccgatactca taatcaccca
     5101 gccggtgtca gtactgtcga atttgatcgc ttctcgccac tcgctctcac tcattccagc
     5161 acgacccgcc ggggtcgaag cgtctgcaat gacgccttta ttcgatgcaa tttccataat
     5221 ttctcgctca atattttgta gggcttatta tttttttccg agccgcatca aggcgatatg
     5281 cggtctacgt ttttgcaggc gagttattag aataaagaaa tgatacgcgc gaggtcagag
     5341 aaaatcttca caatcaagac tttgaaataa cggagatgga taagaatttt ctactaaatt
     5401 aatcgcaaga gaatattcac tctctatttg cggcagatca caaaaaaaag gtgcacattt
     5461 gtgcacccaa ggatgaaagc tgacagcaat gtcagccgca gaccacttta atggccagtc
     5521 cgccgcgtga tgtttcgcgg tatttatcgt tcatatcttt gccggtttca tacatcgtct
     5581 cgatcacttt atcgagtgaa acacgcggtg ccgaggtgcg gcgcatcgcc atccgcgcgg
     5641 cgtttactgc tttcacggca ttaatggcat tacgttcaat gcacgggatt tgtacctgtc
     5701 cggcaaccgg atcgcaggtc agtccaaggt tatgctccat cgcgatttcc gccgcattgc
     5761 atacctgcgc cggactgccg cccagtagtt cagttaaccc tgccgccgcc attgaacagg
     5821 ccacgccaat ctccccctga cagccgacct ccgcgccaga gatggaggcg ttcattttat
     5881 acagcgcgcc aatagccccg gctgcaagaa aatagcgggc aattgaccgc tcgtttaccg
     5941 gacgacggaa cttatcgtaa taagccagca ctgccggaat aatgccgcac gcaccgttag
     6001 tcggtgccgt taccacgcgc ccgccagctg cgttttcttc actaaccgcc agcgcgtaca
     6061 tgttgatcca gtcgatgaca ttcatcggat cgttagagat gttatcgctg gaaaccagct
     6121 gacgacgcag cgctacggca cggcgcggca cattgagcgg accaggcagc acgccttcag
     6181 tgttcatccc gcgttcaata ccgtcatgca tcacttgcca gatacgggca aaaccggcgt
     6241 caatttccgc tttactgcgc agcgctagct cgttgtgcat catcaggcca gaaatagaca
     6301 ggccgttgta atcacacatt ttcagcagtt cacctgctga gtggaaatcg taaggtacgg
     6361 gtgtttcgac atcgtgcgac aggccgaagt gttcttcttc gacaataaac ccgccgccga
     6421 cagagtaata ggttttactt aatagctctt cctgtgcctt ccaggcagtg atccgcattc
     6481 cgttctcatg gcgaggcaac atttcgggat ggaagataat gttctttgct acaggaaaat
     6541 caacaatatg cgcaccagat gccactggca gccgcccgct gcgcgttact aactctataa
     6601 atgcagggat ctcatcaatg acaacatcct gcggactgtt tcctgccagt cccatgatga
     6661 tggcgacatc cgtggcatgg cctttgcccg tcagtgacaa cgacccgtac agatcgacca
     6721 caatatggct cgtcgcggtt aataagccgc tactttccag ccgatcaata aaactttttc
     6781 cggcattcat tggccccacg gtatgcgaac tggagggacc aatcccaatt ttgaaaatat
     6841 cgaatgcact aatcatatcc acaccctcgg attgccgttc agtgaagtgg agcggaacga
     6901 ccttacgacc gtcccgctca cgaggcttta cgcactacgt actgcgatag cttcaatttc
     6961 cagcttcaca tctttcggca aacgcgcgac ctgtacacag ctccgggtcg ggtaggtcgc
     7021 ctgatgctca tcgaagaatt gcttatagac ttcgttgatg gtggcaaaat cattcagatc
     7081 ggtgatgaac acggtcatct tgatgatatc gcccacgctc agcccggcag caaccacgat
     7141 cgctttgacg ttttcgaggc ttaaacgcgc ctgatcttgc acatcagccg ggatctcacc
     7201 ggtctgtggg caaaccggaa tttgaccgga ggtgaagacc atgctgccta aatcaacgcc
     7261 ttgaacataa gggccgattg cgcctggggc acgttgcgtt tcgataatct ttttcataca
     7321 tcctccggcg tcagagcgcc tgggtaaagg tacgtgaaat aacatcctgc tgctgttcac
     7381 gggtcagtgc gttgaagcgc acggcgtagc cagagacacg gattgtcagg ttaggatatt
     7441 tttccgggtg ctcgatggca tccagcagca tttcccgatt cattacgttg acgttgaggt
     7501 gttgaccgcc ttcgacatcc gcttcgtggt ggaaataccc atccagcagg ccgacaaggt
     7561 tggttttacg tactggatct tctttgccca gcgccgcagg gacgattgag aaggtgtacg
     7621 agatcccatc tttggcgtag gtgaacggca gtttcgccac cgacgtcaat gaggccacgg
     7681 cacctttgcg gtcacgacca tgcatcgggt tagcgcccgg cgcgaacggt gttccggcgc
     7741 gacgaccgtc cggcgtgtta ccggttttct ggccgtacac cacgttagaa gtgatagtca
     7801 gaatcgactg ggtagggacg gcgttgcgat aggttggcag cgctttaatt ttcttcataa
     7861 aacgttcaac caggtcgcag gcaatgctgt ctacgcgctc gtcgttgttg ccgtactgcg
     7921 gatattcacc gtcgatttca aagtccaccg ccaggccgtt tttgtcacgg attggtttca
     7981 cgcgggcata tttgatcgca gacagggagt ccgttgccac cgacaggccc gcgatgccgc
     8041 acgccatggt gcgatagaca tcacgatcgt gcagcgccat cagcgaagct tcgtagctgt
     8101 acttgtcgtg catgtagtga atgatattca gcgcgctgat gtactgcacc gccagccagt
     8161 ccatgaagtg atcgaggctg tccatcactt tgtcgtaatc cagcacatcg tccatcaacg
     8221 gtgccgtttt cggcccgacc tgaatcttca gcttctcgtc caccccgccg ttaattgcgt
     8281 acagcagtgt tttcgccagg ttagcgcgtg caccaaagaa ctgcatttgc ttaccaatca
     8341 ccattgggct gacgcagcag gcaatcgcgt aatcgtcgct gttgaagtca gtacgcatca
     8401 gatcgtcgtt ttcatactgc aaggaagagg tgacgatcga cacctgcgcg gcatattttt
     8461 tgaaggcaat cggtaattct tccgaccaaa gaatggtcag gttaggttcc ggtgcaggcc
     8521 ccatagtgtg cagggtgtgc aaatagcgga aggagttttt ggtcaccagc gtacgaccgt
     8581 ccagccccat cccgccgatc acttccgtcg cccagattgg gtcgccggag aacagcgaat
     8641 caaattccgg cgtacgcaag aagcgcacca tacggatctt catgatgaag tgatcgatca
     8701 gttcctgtgc ctgctgctca ttgagtacgc cagctttaaa gtcgcgctca atgtagatat
     8761 ccaggaacga tgccgtgcgg cccagcgaca tcgcgccgcc gttctgtgat ttcactgccg
     8821 ccagataagc gaagtagagc cactgcaccg cttcctgcgc attctgcgcc gggcgagaga
     8881 tatcaaagcc atatttcgcc gccatttcct gaatctgcaa cagcgcatga cgatgctctg
     8941 ccagctcctc acgcagacgg atggtggctt ccagatcctc gcctttttcc agacgagact
     9001 ggagatcggc aaattgcagt tcgcgttcac gcaccagata actgatgcca tacagcgcta
     9061 cgcggcgata gtcaccgata atgcgcccac ggccatagcc atctggtaaa ccggtcagca
     9121 cgccagattt acggcagcgc agcatatccg gtgagtaaac atcaaatacg ccctggttat
     9181 gggttttacg cagatcggta aacagatatt caaattcact gtccatttca cgaccatagg
     9241 cgtggaatga acttttaatc atattaatgc caccgaacgg gtgtaacgca cgtttcaacg
     9301 gcgcatccgt ttgcaggcca acaatttttt ccagcggctg gttaatatat cccgcatcat
     9361 gagcggtaat tgtggtggca atattggtat cgaaatcaac cggcgcgtgg gttgcgtttt
     9421 caatacgaat gccttccatc accttttccc acaattccgt ggtggctggc gtcgcttcag
     9481 cgaggaaaga ttcatcgcct tcatacggtg tatagttatg ttgaataaaa tcgcggacat
     9541 taatttcgtt tttccagtcc gtacctttaa agccaagcca tgcgtcggcg tacagcttat
     9601 cgctggtatc aatatctacc ttcatgaaaa ataatctctc tacaatactt caactaaatt
     9661 atgcaaattc tgcgggtgcg ttaactttgc ctaaatgaat ggcatccaaa gcaatcattt
     9721 tttcttcgtt ggtcggaatt acggcgcaaa tgacacgcgc attttcactg gaaacaattc
     9781 gctcgccaaa agagttagat cgattattca tttctgtatc aatctctacg cctaatacag
     9841 ccaaatgttc catgaccaga cgacgaatta aacttgaatt ctctcctatt ccgccggtga
     9901 atataattcc atccaggcga cgtaatgaag ctgcgtgtcc ggcaatatga cgggcaattc
     9961 ggtgaacaaa ggttttaatt gccagctgcg cgcgttcgtg accttcatgc caggcttttt
    10021 ccagaacacg taaatccgaa gaaagaccgg aaatacctaa taatcccgac tctttattca
    10081 ctacgcgttc caggtcaccc aggctctggt tggtttggct ggcgacccag gacatcgcac
    10141 caaagtcgac atcgccactg cgggtaccca tcatcaagcc ttccagcggc gtcattccca
    10201 ttgaggtgtc aacactctga ccgttgcgaa ctgcgcagat tgacgcgcca ttgccaagat
    10261 gcgccacaac caggccggag tcatcttccg ccagattcag caaagaatgt gcgcgcaggg
    10321 aaacatagcg gtgcgacgtg ccgtggaaac cataacggcg tacacctaac tcttcataat
    10381 atttccacgg caggccgtat aaataagctt ccggagccat cgtctggtgg aaactggtat
    10441 caaataccgc cacctgagtt acgcccggaa ataattgctg cgccgattca ataccactta
    10501 aattggcgta attatgcagg ggtgccagtg gagaaacgcg acggatatta tcaatgactt
    10561 catcggtaat aatggcggac tcggtaaaaa tactgccgcc gtgagcgatg cggtggccaa
    10621 ttaaggccac gctgtcattt aaattccgtt tttccagttc aaatgcaatt gccttcaatg
    10681 caccttcgta gctgtggtga gccagcggtg ctggctctcc cccatttacg gataagaatg
    10741 cattttccga gttaataccg tcggcaatac ctgacattaa tacttcacag tcgctggcat
    10801 cgagtacgga aaacttaatc gaagacgaac cacagttaat aaccaaaaca accggaaatt
    10861 cattcatctc ttttctcatc ctgagttacg gattaaaaca gtttatatac gatgttcagg
    10921 atggtcagca gaccaatcac ggtaacaaac acgttatcca gacgaccacg gtatttcgcc
    10981 agagacggcg ctttacggat ggcatacatc ggcaacaggc acagcaggga tgcgataatc
    11041 ggtgcgccca tggcttcaat caggtcgagg atgttcgggt tggcgtaggc aacaacccag
    11101 gtggagccca tgatgaagat catgctgata gtgttcagtt tacccagcga tactttagtt
    11161 ttgtcgcctt tataaccaaa cttcaggacc aggccattca gaccttccag cgttcccaga
    11221 tagtgaccga agaaagattt gaagatagcc acgagtgcga tgatggaagc cgcatattcc
    11281 agtgtaatcg cgaacgttgt tttggtaccg gtcatggacg caaagtggtt agccagataa
    11341 gaaagcactg gaatattctg cgctttggct tccgccatgt tggccggaga cagcgtaaac
    11401 aggcagctaa aggcaaagaa catcaccact gcaaccatca gcatgctggc acgagaaatg
    11461 atttgggaac atttacgttc ggtgaagtcg cgaccgaagt ctttctcata ctcttcacgt
    11521 ttagaaacca cgaaggaaga gacgattggc gagaagttaa aggagaaaac catgatggaa
    11581 atccccagcc agacagtgat caggataccg tcatgaccgg ttaacgacag cgaaccgagg
    11641 tcaacctggt cgataactgc agagttccag taagggatca gcgacaaaga aatcagcacc
    11701 aggctggcga taaacggcca taccaggtag ctcatcactt taaccatcag atccttacca
    11761 aaccagatga cgaaagccat cagcagcaac aggaacagcg ccacaaagcc gcgattcagc
    11821 ggtgcaaagc cgagctggtt ttcccagaac gtcataaagg tattggtaat agtaacgcca
    11881 taaatccaca gcagtgggca aatcgcgaag aagtacagga acgtgataac cacgccgcca
    11941 gttttaccaa aatgctcttc caccgtttcc gtaatgttgc cggaagggtt agagccagaa
    12001 agacacagac gcgccagcgc ccggtggcaa taaaatgcga tggggtatgc caataccaac
    12061 atcagaagaa tcgggatcag tccgccaaaa cctgcgcgga tagggaagaa cagcaccccg
    12121 gcgccgattg ccgtaccaaa caagcctaac gtccatgtgg tatctgattt acgccaggac
    12181 gattgttttg tctggctgga tacaatgcta tctgaagtac tcatatccta tcctcaacga
    12241 attaattaag cgtcaacgaa accggtgatt tgagagacgc gagaaagatc gatattgccg
    12301 ccggaaataa tactgacggt ttttctgttt tgaatatatt ggtctaattt accgcttaat
    12361 aatgcagcac atgccagagc gcctgcgcct tcggtgacga ctttattgcg ctgaattaag
    12421 gcaatcatac tgtttctgat ttcgtcttcg ctgaccagca cgatgtcatc gactaattca
    12481 cgaacgattt cgtaagttaa attacccggg cgggagacat cacaaccatc cgccagggtg
    12541 ccggtagttc ggtgcgtggt tatttctccg gagtggaaag aagccgccat gccgtgaacg
    12601 ttttcagact gtacgccaat aacacgaatg gtcgggttaa tagatttaat tgccaccgca
    12661 ataccagcaa ttaaaccgcc accaccaatt ggcacaatca cgttatcgac atcatagaga
    12721 tcttccataa tttccaggcc aatcgttccc tgaccagcaa tcactttcgg atcatcgtaa
    12781 ggtgggataa aaatacggcc ttccatttcg acaatttcgc tcactttagc gatagtgtcg
    12841 ttgaagttat caccatgcag aacgacttct gcggagtagt cgcacgttgc cgctactttg
    12901 gatttcggcg cgccttttgg catcaccact ttaccgtcga tacccagcat cgcgcaggag
    12961 agcgaaaccc cttgcgcatg gttgcccgca gaacaggcca ccacgccttt gcgtttttcc
    13021 gcatcagtca gtgaacttaa tttattaaat gcgccacgaa ttttaaatga acccgtacgc
    13081 tgcatatttt caaacttcag gaatatttca cctttgcaac gttcactaaa atagttggag
    13141 cgaggcatgc ctgttttata aattcgccca gccagtcgtt gtttcgcttc aataatgtca
    13201 tcaatagcaa ccggcagatc gtatgtaata tgcattataa aacctcttcg cctgaattaa
    13261 atatgtaggt aaccgtaacc gacacctgca agacagacag gtggattatt tatgtttaaa
    13321 taaaaacaaa taactaacca acttctatta attgccttcg tctacaccca ttataagatg
    13381 aatactcttt ggctaattcc accaaaaccg atgctgcttt tttaatacga taatttttcg
    13441 accataccgc ggcatattgt gccacaggta atgtttcttc aaccggaata gtaataaatt
    13501 gattagaacc aaaaggagac gtcatatcac aaggaattac agttaagaaa tcagcattga
    13561 gaacaagatt ataaattgtc acgactgagt cggttttaac gatgttttca atactgatgc
    13621 catttctttg taacgtagta agcagttcgc tgtagtaccc catattagtt tgtggcaaca
    13681 cccactgttc gttcttcaac gactccagcg tggtggtgcc ggtgcatgtt cgggacttac
    13741 tggctaccag cacaaactcg gactcgaaca gcggctcaac atgtaaatcc tgaagcttca
    13801 tttctgcgct taacgtacca atcgcaaaat caagtcgacc gtcgcggatt gccggtaaga
    13861 acgaagacag ttgcgcttca tacatagaaa cctgcgcttt cgggaacacc tctttgaact
    13921 tgttgatcat ccctgacata aaagtaaaac caatcaatga aggaaaacca aatgagactt
    13981 ccaccaccgc ctcagaagac ataccgctta tctcattaac catatttttc atttcacggg
    14041 taatggattc ggaacgagag agtaacaatt gaccggcagg tgttaatgtt actccggtat
    14101 ttttccgcac cactaattcc acaccaaaat aatcttcaat atcgttaatg attttactga
    14161 cggccggttg agttaaccct aattcttttg cagccgagcc gatagaacca cttctaatga
    14221 cttcctgaaa gactaccagg tgctgcgttt tcggaagaag aatagtgctc ataacgacct
    14281 acgttaatta cctcattgac ggcatgaagt gtatcaaaat gaaatgaaca ggatatgtgc
    14341 gaccactcac aaatta
//
LOCUS       DAZKIP010000109        14277 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZKIP010000109 DAZKIP010000000
VERSION     DAZKIP010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.110"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            256..1827
                     /gene="garD"
                     /locus_tag="ACHOUK_003603"
     CDS             256..1827
                     /gene="garD"
                     /locus_tag="ACHOUK_003603"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFP9898974.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPKAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            1976..2311
                     /gene="prlF"
                     /locus_tag="ACHOUK_003604"
     CDS             1976..2311
                     /gene="prlF"
                     /locus_tag="ACHOUK_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFP9898975.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            2311..2775
                     /gene="yhaV"
                     /locus_tag="ACHOUK_003605"
     CDS             2311..2775
                     /gene="yhaV"
                     /locus_tag="ACHOUK_003605"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFP9898976.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH"
     gene            complement(2830..3639)
                     /gene="agaR"
                     /locus_tag="ACHOUK_003606"
     CDS             complement(2830..3639)
                     /gene="agaR"
                     /locus_tag="ACHOUK_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFP9898977.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            3888..5168
                     /gene="kbaZ"
                     /locus_tag="ACHOUK_003607"
     CDS             3888..5168
                     /gene="kbaZ"
                     /locus_tag="ACHOUK_003607"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFP9898978.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKATALSQMVESYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLNRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            5191..5664
                     /gene="agaV"
                     /locus_tag="ACHOUK_003608"
     CDS             5191..5664
                     /gene="agaV"
                     /locus_tag="ACHOUK_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFP9898979.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGIPTEPAVDLFKLL"
     gene            5675..6454
                     /gene="agaW"
                     /locus_tag="ACHOUK_003609"
     CDS             5675..6454
                     /gene="agaW"
                     /locus_tag="ACHOUK_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFP9898980.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            6444..7322
                     /gene="agaE"
                     /locus_tag="ACHOUK_003610"
     CDS             6444..7322
                     /gene="agaE"
                     /locus_tag="ACHOUK_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFP9898981.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            7340..7774
                     /gene="agaF"
                     /locus_tag="ACHOUK_003611"
     CDS             7340..7774
                     /gene="agaF"
                     /locus_tag="ACHOUK_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFP9898982.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            7771..8904
                     /gene="nagA"
                     /locus_tag="ACHOUK_003612"
     CDS             7771..8904
                     /gene="nagA"
                     /locus_tag="ACHOUK_003612"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFP9898983.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVSVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLSTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            9255..10409
                     /gene="agaS"
                     /locus_tag="ACHOUK_003613"
     CDS             9255..10409
                     /gene="agaS"
                     /locus_tag="ACHOUK_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFP9898984.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALHSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFAVL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELHRDNQAMRVIAIAAESSDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            10422..11282
                     /gene="kbaY"
                     /locus_tag="ACHOUK_003614"
     CDS             10422..11282
                     /gene="kbaY"
                     /locus_tag="ACHOUK_003614"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFP9898985.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            11449..11925
                     /gene="agaB"
                     /locus_tag="ACHOUK_003615"
     CDS             11449..11925
                     /gene="agaB"
                     /locus_tag="ACHOUK_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFP9898986.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLTDLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            11997..12767
                     /gene="agaC"
                     /locus_tag="ACHOUK_003616"
     CDS             11997..12767
                     /gene="agaC"
                     /locus_tag="ACHOUK_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFP9898987.1"
                     /translation="MAALVFVLGIDFWLEALFLFRPIIVCTLTGAILGDIQTGLITGG
                     LTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLPFSLLMQYVILF
                     FYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCTYLAQGAMQALV
                     KAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVCYIQVSNLLPVA
                     VLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            12757..13548
                     /gene="agaD"
                     /locus_tag="ACHOUK_003617"
     CDS             12757..13548
                     /gene="agaD"
                     /locus_tag="ACHOUK_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFP9898988.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFSLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            13549..14277
                     /gene="agaI"
                     /locus_tag="ACHOUK_003618"
                     /pseudo
     CDS             13549..14277
                     /gene="agaI"
                     /locus_tag="ACHOUK_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417610.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
BASE COUNT         3436 a         3608 c         3739 g         3494 t
ORIGIN      
        1 ataatcattg tgcaaatgct aatttaatta atactattta aatattattt tgagcatatg
       61 cacataaggt tgcgcgctaa agcacagatt tgcgctttac cttgccgggc ggcattgcaa
      121 tccctgaaat gattgacatt gatcacattt ctgcgtttaa actcctgaca ttcttatttc
      181 acccaatgaa gtcatttatt tttaaatgag accaggtcct cattttaata acctctggct
      241 ggagaatatt gcacaatggc caacatcgaa atcagacaag aaacgccaac tgcgttttat
      301 ataaaagttc acgacacaga taatgtggca attattgtta atgataatgg cctgaaagca
      361 ggaacgcgtt ttccggatgg gctggaatta attgaacata ttccccaggg gcataaagtc
      421 gcattgctcg acattccggc taatggtgaa attattcgtt atggcgaagt gattggttac
      481 gccgtgcgtg caatcccacg cggaagctgg atcgacgaat caatggttgt actgccgaaa
      541 gcgccgccgt tacacacgct gccactggca accaaagtcc cggaaccctt accgccgctg
      601 gaaggataca cctttgaggg ctatcgcaat gccgatggca gcgtgggcac caaaaacctg
      661 ctcggcatca ccaccagcgt ccactgtgtg gcaggcgtgg tggactacgt agtaaaaatc
      721 attgaacgcg atctgctacc gaaatacccg aacgtcgatg gcgtggtggg gctgaatcat
      781 ttgtacggtt gtggcgtggc gattaacgca ccggcggcag ttgtacctat ccgtaccatt
      841 cacaatattt cgctgaatcc taactttggc ggcgaagtaa tggtgattgg cctgggttgt
      901 gaaaagttgc agcctgagcg cctgctgact ggaacggatg atgtgcaagc tattccagta
      961 gaaagcgcca gcattgtcag tttgcaggat gaaaagcatg tcggttttca gtccatggtc
     1021 gaggatattt tgcaggtcgc cgaacgccat ctacaaaaac tgaatcaacg tcagcgagaa
     1081 acctgtccgg cttcagaact ggttgtcggc atgcagtgcg gtggcagcga tgccttttct
     1141 ggcgtaacgg caaacccggc ggttgggtat gcgtctgatc tactggtgcg ctgcggcgca
     1201 acggtgatgt tctcagaagt cacggaagtg cgtgacgcga tccatctgct gacaccacgc
     1261 gcagtgaacg aagaggtcgg caaacggctg ctggaggaga tggagtggta cgataactat
     1321 ctcaatatgg gaaaaaccga ccgcagcgcc aacccttcgc cgggcaacaa gaaaggcggt
     1381 ctggcaaatg tagtggagaa agcgctcggc tccattgcta aatcgggtaa aagtgcaatt
     1441 gttgaagtgc tgtcgcccgg tcaacgcccg actaaacgcg gattaattta cgccgcgacg
     1501 ccagccagcg attttgtctg tggcacgcaa caggtggctt cgggtatcac cgtgcaagtg
     1561 tttacgaccg gtcgtggtac gccgtacggc ctgatggcgg tacccgtcat taaaatggcg
     1621 acccgcaccg agctggcgaa ccgctggttt gatttaatgg atattaacgc gggcactatc
     1681 gccaccggcg aagaaaccat tgaagaggtg ggctggaagt tgttccactt tattctcgac
     1741 gtcgccagcg ggaagaagaa aaccttctcg gatcaatggg gactgcataa ccagctggcg
     1801 gtgtttaacc cagcaccggt gacctgattt cttttggaac gtccctcgca aaacatggcc
     1861 ttagtgccat gtttttattg tttaaagccc ccacgtccat taataatgca tttgcattac
     1921 cttagttcaa gcttataatt tgagcagaaa acaggctgta aaaggacagt gaatcatgcc
     1981 cgctaatgct cgctctcacg ctgtactgac cactgaatca aaggtcacga tacgcggaca
     2041 aacaactatc cccgcgccag tgcgtgaggc cttaaaactg aagccaggcc aggacagcat
     2101 tcattacgaa attctgcctg gtgggcaagt atttatgtgc cgactgggag atgagcagga
     2161 ggatcatact atgaatgcat ttttgcgttt tctggataca gatatccaga acaacccgca
     2221 aaaaactcgt ccattcaaca ttcaacaagg aaagaaactt gtcgctggca tggacgtcaa
     2281 cattgatgat gagattggcg acgacgaata atggattttc cacaaagggt taatggttgg
     2341 gcgctatatg ctcatccctg ttttcaggaa acctacgacg ctttagttgc cgaagtcgag
     2401 acattaaagg gaaaagatcc tgaaaattat cagagaaaag ctgccacaaa gttattggcg
     2461 gtggtccata aagtgattga ggagcatatc acggtcaatc catcatcacc ggcattccgt
     2521 catggcaagt cgttaggctc tgggaaaaat aaagactggt cacgggtaaa atttggtgct
     2581 ggtcgctatc gtctcttctt tcgttacagt gaaaaagaga aagtcatcat tctgggatgg
     2641 atgaacgatg aaaacacgct gcgcacctac ggtaaaaaaa cagatgccta taccgtattc
     2701 agcaaaatgt taaaaagagg acatcctcct gccgactggg aaaccctcac ccgagagaca
     2761 gaagaaaccc attgatggtg ttcacaatgc ccgccgaacg cattcgtgtt gaccagcggg
     2821 caccctttct cactccccga ccagaatcac ttcaacccca gcctttcgca gcccttccag
     2881 gctatccgca ggaatgcctt catcaacaat gatcatgtcg atacgttgag tatcaatgat
     2941 cttatgtaaa ctggaacgat tgaatttact ggaatcggtg accacgatga tccgttccgc
     3001 aacttcgcac atccggcggt ttaaacgggc ttcatcttca ttatgcgtgc tgacgccgcg
     3061 ctccagatcg atcgcatcta caccaagaaa cagcatatcg aagtggtaat tttgcagcga
     3121 ttgctcagcc tgatcgccgt aaaaagattg cgactgacgg cgcaaatgcc cgccggtcat
     3181 cagcagctca acgccttccg cttccagcaa cgcattagcc acgttcatac cgttggtcat
     3241 cgcaattacg tcagtgtgct tgcgcatcag acgagcaatc tcaaaagtgg tggtcccgga
     3301 atcgaggatc acccgatgac ctggctgaat caactcaacg gcagctttcg caacgctgcg
     3361 tttcattgcg gtgttcagcg cgcttttatc ttccactgat ggctcgactg acggcgttgt
     3421 gctatcgcag atcaacgcgc caccataggc acgcacagcg atcccctgct tttccagaaa
     3481 cgccagatcg ttgcggatcg tcacggtaga tacgccatac aatgccgaca gatcgttaac
     3541 ctgcacactc ccttgctgtc gcagacgctg aatgatctgt tctcgtcgct cgctggtgcc
     3601 tgtcactcgc ttctcacctg aagcgtcggt attactcata gtaagtcctt tcgtaaaact
     3661 ttcgtttcat ttcgttttgc ctattaacgc ctttctatta agcaaatgca agcccacctt
     3721 gcccattgac gcaagctact ctcgtttcag tgactttcat tatgtttctt ttgtgaatca
     3781 gatcagaaaa ctattatctt tcgttttatt tttatctcac catgacgcag tatcaactga
     3841 aacaaaacga aagattaata tcgcagtaat ctgaactgga gaggaaagtg aaacatctga
     3901 cagaaatggt gagacagcac aaagcgggca aaacaaatgg aatttatgcc gtttgttccg
     3961 cacatccgct ggtgctggag gctgcaatcc gctacgccag tgcaaaccaa acgccgttac
     4021 tgattgaagc aacctccaat caggtagacc agttcggcgg ttataccgga atgacgcccg
     4081 ccgattttcg cggctttgtt tgtcagctcg ccgactcgtt gaatttcccg caggatgcgt
     4141 tgattctggg tggtgaccat ctggggccaa accgctggca aaacctgccg gccgctcagg
     4201 caatggccaa tgccgatgat ttgattaaaa gctacgttgc ggcaggattc aaaaaaatcc
     4261 accttgattg cagcatgtcc tgtcaggacg atccgattcc cttaactgat gacatcgtgg
     4321 ctgaacgcgc cgcccgtctg gcgaaagtgg cggaagaaac ctgtcttgaa cactttggcg
     4381 aagccgatct ggagtatgtc attggtaccg aagtgccggt acctggcggc gcgcatgaaa
     4441 ccttaagcga gctggcggtc accacgccgg atgccgcccg cgccacgctg gaagcccatc
     4501 gtcacgcctt tgaaaagcaa ggtttgaatg ccatctggcc acgcatcatt gccctggtgg
     4561 ttcaacccgg cgtcgaattc gatcacacca acgttattga ttatcagccc gccaaagcga
     4621 ccgccttaag ccagatggtc gaaagctacg aaacgctgat tttcgaagcg cactctaccg
     4681 attatcaaac gccgcaatcg ctgcgccagc tggtgattga ccactttgcc attctgaaag
     4741 ttggcccggc gctgaccttc gccctgcgtg aagctctgtt ctctctggca gcgattgaag
     4801 aagaactggt gccagcaaaa gcctgttctg gtctgcgtca ggtgctggaa aacgtgatgc
     4861 tcaaccgccc ggaatactgg caaagccact accacggtga cggcaacgcg cgtcgtctgg
     4921 cgcgtggtta tagctactcg gatcgcgtgc gctattactg gccggacagc cagattgatg
     4981 acgctttcgc tcatctggta cgtaatctgg cggattcacc aattccgctg ccgctgatca
     5041 gccagtatct gccgctgcag tacgtgaaag ttcgctccgg cgagctgcag ccaacgccac
     5101 gggaactcat tatcaaccat attcaggaca tcctggcgca gtaccacaca gcctgtgaag
     5161 gccaataagc aaaacaaaga ggaacacgct atgccaaata ttgttttaag ccggattgat
     5221 gaacgcttga ttcacggtca ggtcggcgtt cagtgggtcg gatttgcggg ggcaaatctg
     5281 gtgctggtcg ccaacgatga ggttgccgaa gatccggtac aacaaaacct gatggaaatg
     5341 gtactggcag aagggatcgc cgtacgtttc tggacgctgc aaaaagttat cgacaacatt
     5401 catcgcgccg ccgatcgaca gaaaatcctg ctggtttgta aaacacccgc cgatttcctg
     5461 acgctggtga aaggtggcgt tccggtgaat cgcattaacg ttggcaatat gcactacgcc
     5521 aatggcaagc aacaaatcgc caaaacggtt tctgtggatg cgggcgatat cgcagcattt
     5581 aacgacctga aagccgctgg ggtggaatgc ttcgttcagg gcatcccgac agagcctgct
     5641 gtggacctct ttaaattact ttgagggatt catcatggaa atcagtctgt tgcaggcatt
     5701 cgcgttgggc attatcgcct ttatcgctgg cctggatatg tttaacggct taacccatat
     5761 gcaccgccca gtggtcctcg gcccgttagt cgggctggta cttggcgatc tgcataccgg
     5821 tattttaacc ggcggtacgc tggaactggt gtggatgggg ctggccccgc tggcgggcgc
     5881 acagccgcct aatgtgatta tcggtactat cgtcggcacg gcgtttgcca ttactactgg
     5941 cgtgaaaccc gatgtcgcag tcggtgtcgc cgtacctttc gctgtcgcag tacagatggg
     6001 gattaccttc ctgttctcgg tgatgtctgg cgtgatgtct cgctgcgacc ggatggcgga
     6061 gaacgccgat actcgcggca ttgaacgcgt aaactatctg gcactgctgg cactcggcac
     6121 tttctatttt ctctgcgctt tcctgcctat ctacttcggc gcggaacatg caaaaaccat
     6181 cattgatgtc ctgccgcaac gattaatcga cggcctcggc gtcgcgggcg gcatcatgcc
     6241 agcaatcggt tttgccgtgc tgctgaaaat catgatgaaa aacgtctaca tcccctactt
     6301 catcctgggc tttgttgccg cagcctggct caagttaccg gtgctggcta ttgctgccgc
     6361 cgcgctggcg atggcgctga tcgatctgct gcgtaaatct cctgaaccga ctcaacctgc
     6421 ggcacagaaa gaggaatttg aagatggcat ctaatcaaac caccctgccg aacgtcagtg
     6481 aaaacgaaga aacattgctg actggcgtca atgaaaacgt gtatgaagat cagagcattg
     6541 gcgcggagct gacgaaaaaa gatatcaatc gcgtcgcctg gcgttccatg ctgttacaag
     6601 cgtcttttaa ctacgaacgt atgcaggctt ccggctggtt gtacggtctg ctgcctgctc
     6661 tgaaaaagat ccacactaat aaacgcgacc tggcgcgcgc catgaagggg catatgggtt
     6721 tcttcaatac ccatccgttt ctggtgacat ttgttatcgg cattatcctt gcgatggagc
     6781 gttctaagca ggacgttaac agtattcaga gcaccaaaat tgccgtcggt gcgccgctcg
     6841 gcgggattgg cgatgcaatg ttctggctaa cgctactgcc gatttgtggc gggattggag
     6901 ccagtctggc attgcaaggc tccattcttg gcgcagtcgt ctttattgtg ctgttcaacg
     6961 tggtccacct ggggctgcgt tttggtctgg cgcattatgc ttaccgcatg ggcgtggcgg
     7021 cgattccact cattaaagct aataccaaaa aagtcggcca tgctgcgtct atcgttggga
     7081 tgacggtaat cggtgcgctg gtggcaacct atgttcgttt aagcaccacg ctggaaatca
     7141 cagcgggcga cgcagtggtt aagttacagg ctgatgttat cgacaaactg atgcctgcct
     7201 tcttaccgtt ggtctacacc ctgaccatgt tttggttggt acgccgcggc tggagtccgc
     7261 tgcgtctgat tgcagttacc gtggttctcg gcatcgttgg taaattctgc catttccttt
     7321 aaatacaatg aggtattaga tgttaagtat tattttgaca gggcatggcg ggtttgccag
     7381 cggcatggaa aaagcaatga agcaaattct cggtgagcaa tcgcagttta tcgccatcga
     7441 ttttccggaa acctcaagca ccgcgctgct tacatcgcag cttgaagagg ccatcgccca
     7501 actggattgt gaagacggca ttgtttttct gacggatttg ctgggcggca cgccgtttcg
     7561 cgtagcttca acgctggcga tgcaaaaacc gggctgtgaa gtgatcaccg gcaccaattt
     7621 gcaactgcta ctggagatgg tgctggagcg cgaagggtta agcggtgaag aattccgtgt
     7681 acaggcgctg gagtgtggac atcgtgggct gaccagtctg gtggacgagt taggccgctg
     7741 ccatgaagaa tgtccggtcg aggaagggat atgacacacg ttctgcgcgc cagaaggctg
     7801 ctgactgaag agggatggct cgatgaccat cagttgcgta ttgctgacgg tgtcatcgca
     7861 gcaatcgaac cgattccagt gagcgtgact gaacgcgatg cggaactgct ctgccccgct
     7921 tacatcgaca cccatgtaca cggtggtgcg ggcgttgatg taatggatga cgcgccggat
     7981 gtactcgaca agctggcaat gcacaaggca cgcgaaggtg tcggcagttg gttgccgact
     8041 accgtaaccg cgccgcttag taccattcat gcggcgctga aacgtattgc tcaacgttgc
     8101 caacgcggcg gacctggtgc gcaagtgctg gggagttatc tcgaaggacc gtacttcacg
     8161 ccgcagaata aaggcgcgca tccgccggag ttgtttcgcg agcttgaaat tgccgagctg
     8221 gatcaattga ttgccgtttc tcagcacacc ttacgcgtgg tagcgctggc accggaaaaa
     8281 gagggggcat tgcaggccat ccgccatctt aaacagcaaa atgtacgagt gatgctgggg
     8341 catagcgcgg cgacctggca acaaactcgc gccgcgtttg atgctggtgc cgacggcctg
     8401 gtgcattgct ataacgggat gacaggttta catcaccgcg aaccgggaat ggttggcgcg
     8461 ggattaacgg acaagcgcgc ctggctggaa ctgatagccg atggtcatca tgtgcatccg
     8521 gcggcaatgt cgctgtgttg ttgctgtgcg aaagagagaa tcgtactgat caccgacgcg
     8581 atgcaggcag ccgggatgcc ggatggtcgc tatacgttat gtggcgaaga agtgcagatg
     8641 cacggtggcg ttgtccgtac cgcgtccggt gggctggcgg gcagtacgct gtctgttgat
     8701 gcggcagtgc gcaacatggt cgagttgacg ggcgtaacgc ctgcggaagc cattcatatg
     8761 gcgtcgctgc atccggcgcg aatgctgggt gttgatggtg ttctgggatc gcttaaaccg
     8821 ggcaaacgcg ccagcgtcgt tgcgctggat agcgggctgc atgtgcaaca aatctggatt
     8881 cagggtcaat tagcttcgtt ttgatggttt gctccattat tgggccttca cttcccccgt
     8941 aaggcctttc tttttctttc gttttgatct gtgcagcggt gtcggatgcg acgctaacgc
     9001 gtcttatccg acctacagtt ggtgaccgca aggccggata aagcgtttgc gccgcatcca
     9061 gcaatccctt ttgcttcctt tatcttttct ttcaacgatc acggatttcg ttttatttcc
     9121 tttttctcca ttgaactttc ggtttctttt ctatagattt taatcaacga aagacatcac
     9181 caagtgaaat gaaacgaaag gcaagtgaaa gcgacaacgc ccgacgtcaa gttcatcaga
     9241 ctaaggattg agttatgcca gaaaattaca cccctgctgc cgccgcaacc ggtacatgga
     9301 ctgaagaaga gatccgccat cagcctcgcg catggatccg ttcgctcacc aacatcgacg
     9361 cgctacattc cgcgcttaat aacttccttg aaccgttact gcgcaaagag aatctgcgga
     9421 tcatcctgac cggagccgga acctcggcat ttatcggtga catcatcgcg ccgtggctcg
     9481 ccagccatac cggtaaaaac ttcagcgccg taccgaccac cgatctggtc actaatccga
     9541 tggactacct gaacccagcc catccgctgc tgttgatctc cttcggtcga tccggcaaca
     9601 gcccggaaag cgtcgcagcc gtggaactgg caaatcaatt tgtaccggaa tgctatcacc
     9661 tgccgatcac ctgcaacgaa gcgggcgctc tttaccaaaa cgcgatcaac agcgataacg
     9721 cgtttgccgt gctgatgccc gcagaaacgc acgatcgcgg ctttgcgatg accagcagca
     9781 ttaccaccat gatggccagc tgcctcgcgg ttttcgcacc tgagacgatc aacagccaaa
     9841 ccttccgcga cgtggcggat cgttgccagg cgatcctgac ctcactgggc gatttcagcg
     9901 aaggtgtgtt tggttacgca ccgtggaaac ggatcgttta tctcggcagc ggtggcttac
     9961 agggcgcagc acgcgagtcg gcgctgaaag tgctggaact gacggcgggt aaactggcgg
    10021 ccttttatga ttctccaacc ggattccgtc atggaccaaa atcgctggtc gataacgaaa
    10081 cactggtggt ggtatttgtc tccagccacc cttacacccg tcagtatgat cttgatctgc
    10141 tggctgaact tcaccgtgac aaccaggcaa tgcgtgtaat cgccatcgcc gcggaaagca
    10201 gcgacatcgt cgctgccggt ccacatatca tcctgccacc gtcacgtcac tttatcgacg
    10261 ttgagcaggc attttgcttc ctgatgtacg cccagacgtt tgcactgatg cagtcgctgc
    10321 acatgggcaa tacgccggat accccatcag ccagtggcac cgttaaccgc gtggtgcaag
    10381 gcgtaatcat tcatccgtgg caggcataag aggatcgcat tatgagcatt atctccacca
    10441 aatatctgtt acaggacgcc caggccaatg gctacgcggt gcctgctttt aacattcata
    10501 acgccgagac gatccaggcg atcctcgaag tgtgcagtga aatgcgatcg ccggtgatcc
    10561 tcgccggaac gccggggacc tttaaacata tcgcgctgga agagatctac gccctgtgca
    10621 gcgcctattc cacaacctac aacatgccac tggcgctgca tctcgaccac cacgaatcgc
    10681 tggatgatat tcgccgtaaa gtccacgcag gtgtgcgcag tgcgatgatc gacggcagcc
    10741 acttcccgtt tgccgagaac gtaaagctgg tgaaatcggt tgtcgacttc tgccactccc
    10801 aggattgcag cgtggaagca gaactgggcc gcctgggcgg tgttgaagat gacatgagcg
    10861 ttgacgccga aagtgcattc ctgaccgatc cacaagaagc taaacgcttt gtcgaactga
    10921 ctggcgtcga cagcttggcg gtagcgattg gtacggcgca cggcttatac agcaaaacgc
    10981 cgaagattga tttccagcgg ctggcggaaa ttcgtgaagt ggtggatgtt cctctggtgc
    11041 tacatggtgc cagcgatgtt ccggatgaat ttgtccgtcg cactattgaa cttggcgtca
    11101 caaaagtgaa cgttgccaca gaattaaaaa tagccttcgc cggcgcggtt aaagcctggt
    11161 ttgcggaaaa tccgcagggt aatgatcctc gttattatat gcgcgtcgga atggatgcga
    11221 tgaaagaagt tgtcagaaat aaaattaatg tctgtggttc agcgaatcga atttcagcat
    11281 aatcattagt tttttatgat ttatcccaat gtacttcccg attaatatcg gggagtgcct
    11341 taatggaaaa ggagatgact aaaccttaat aaatagatca gtacaatatc gcaacaataa
    11401 tatatttaaa aaaattatat tattcaactt tatggtgagg attacacaat gagcagtcca
    11461 aatattctct taacccgtat tgataaccgt ctggttcacg gtcaggttgg cgtgacctgg
    11521 acatccacca tcggtgcgaa tctgctggta gtcgtggatg atgttgtcgc taacgatgat
    11581 attcaacaga aattaatggg tattaccgca gaaacctacg gctttggcat tcgtttcttt
    11641 actatcgaaa aaaccattaa cgtcatcggc aaagctgcac cgcatcagaa gatcttcctg
    11701 atttgccgta cgccacaaac ggtacgtaaa ttggtagaag gtggcattga cctgaaagat
    11761 gtcaacgtcg gcaatatgca tttctcggaa gggaaaaagc aaatcagcag taaagtttat
    11821 gtcgatgacc aggatctcac ggacttacgt tttattaaac aacgtggcgt gaatgttttc
    11881 attcaggacg tccctggcga tcaaaaagaa caaatccctg actaaatcta aaatcgcctt
    11941 aatattggtt tgaggtaata aaaatgcatg aaataaccct actttaggga ttatccctgg
    12001 cggcgttagt tttcgttctg gggattgatt tttggctgga agccttattt ttattccgcc
    12061 cgataatcgt ttgtacccta actggcgcta ttctcggtga tattcagact ggcttaatta
    12121 ccggtggtct gacagagttg gctttcgccg gattaacccc tgcaggtggt gttcagccgc
    12181 ccaacccgat tatggcgggt ctgatgacca ccgtcattgc atggtctacg ggcgttgatg
    12241 ccaaaacggc aattggtctt ggcctgccgt ttagtttgtt aatgcagtac gtcattctgt
    12301 tcttctattc tgctttctca ttatttatga ccaaagccga taaatgcgcg aaagaggcgg
    12361 atacggcagc gttttcccga cttaactgga caacgatgct catcgtcgct tcagcgtatg
    12421 cggtgattgc tttcctctgt acttacctgg cacaaggggc gatgcaggcg ctggtgaaag
    12481 cgatgcccgc ttggctgacc cacggctttg aagtggctgg cggtattctg cctgccgttg
    12541 gttttggctt gctgctgcgc gtgatgttca aagcacaata tatcccttac ctgatcgccg
    12601 gcttcctgtt tgtttgctac atccaggtca gcaacctgtt gccggttgcc gtactgggcg
    12661 caggctttgc ggtgtatgag tttttcaatg cgaaatcccg gcagcaagcg caaccgcagc
    12721 ccgttgccag taaaaatgaa gaagaggact acagcaatgg gatctgaaat cagtaaaaaa
    12781 gatatcaccc gtctgggctt tcgttcgtcg ctgctgcaag cgagctttaa ctacgaaagg
    12841 atgcaagcgg gcggttttac ctgggcgatg ttgccgatcc tgaaaaagat ttataaggat
    12901 gacaaaccgg gcctaagcgc agcgatgaaa gataacctcg aatttattaa tacccacccg
    12961 aatctggtcg gattcctgat ggggttatta atttcgatgg aagaaaaagg agaaaaccgc
    13021 gacaccatta aaggcctcaa agtggcactg tttggcccaa tcgccgggat tggcgatgcg
    13081 attttctggt ttactttgtt gccgattatg gcgggaattt gctcatcatt tgccagccag
    13141 ggaaacctgc tggggccgat tctatttttc gccgtttacc tgcttatctt ttccctgcgc
    13201 gtcggctgga cccacgtcgg ttattcagtc ggcgtgaagg cgatcgataa agtgcgagag
    13261 aactcgcaga tgattgctcg ctcggcaacc atcctcggga tcacggtaat cggcgggctg
    13321 atcgcttcgt atgtgcatat taacgtggtg acatcgtttg ccatcgacag tacccacagc
    13381 gtcgcgctgc aacaggattt cttcgataaa gtcttcccga acattttacc gatggcctac
    13441 accctgctga tgtattactt cctgcgggtg aaaaaagcgc atccggtgct gttaatcggc
    13501 gtgacttttg tgctctctat tgtttgttcc gcattcggca ttttgtaaat ggaacgaggc
    13561 actgcgtctg gcggtgcctc tttacttaag gaatttcatc ctgtgcaaac ccttcagcaa
    13621 gttgaaaact atacggcgtt aagtgaacgt gccagcgaat atttattggc cgtgatccgt
    13681 agcaaaccgg atgccgtgat ttgcctggcg accggagcca cgccattact gacgtatcat
    13741 tatctggtag aaaaaatcca ctagcagcag gttgatgtca gccagctcac cttcgtgaag
    13801 ctcgacgaat gggtggatct gccattaacg atgcctggta cctgcgaaac tttcctgcaa
    13861 cagcatatcg tgcagccgct ggggctacgt gaagaccagc tcatcagctt tcgctccgaa
    13921 gagataaatg agacagagtg cgaacgggta acgaacctga ttgcgcgcaa aggcggtctg
    13981 gatttatgcg ttctcggatt ggggaaaaac ggtcatcttg ggctgaacga accgggagaa
    14041 aacctgcaac cggcctgcca tatcagtcaa cttgatgcca gaacacagca acatgagatg
    14101 ttaaaaaccg cgggtcgccc cgtgactcgt gggatcacct taggcctgaa ggatattctc
    14161 aatgcccgcg aggttttgtt actggtgact ggcgaaggaa agcaggatgc gacagaacgt
    14221 tttctcacgg ctaaagtctc tacctctatc ccggcttcgt ttttgtggct gcacagt
//
LOCUS       DAZKIP010000110        14209 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZKIP010000110 DAZKIP010000000
VERSION     DAZKIP010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14209
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.111"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            147..1205
                     /gene="citC"
                     /locus_tag="ACHOUK_003619"
     CDS             147..1205
                     /gene="citC"
                     /locus_tag="ACHOUK_003619"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFP9898989.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIAMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1220..1516
                     /gene="citD"
                     /locus_tag="ACHOUK_003620"
     CDS             1220..1516
                     /gene="citD"
                     /locus_tag="ACHOUK_003620"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFP9898990.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1513..2421
                     /gene="citE"
                     /locus_tag="ACHOUK_003621"
     CDS             1513..2421
                     /gene="citE"
                     /locus_tag="ACHOUK_003621"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFP9898991.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2432..3964
                     /gene="citF"
                     /locus_tag="ACHOUK_003622"
     CDS             2432..3964
                     /gene="citF"
                     /locus_tag="ACHOUK_003622"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFP9898992.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLILASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTLFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3968..4519
                     /gene="citX"
                     /locus_tag="ACHOUK_003623"
     CDS             3968..4519
                     /gene="citX"
                     /locus_tag="ACHOUK_003623"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFP9898993.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4494..5372
                     /gene="citG"
                     /locus_tag="ACHOUK_003624"
     CDS             4494..5372
                     /gene="citG"
                     /locus_tag="ACHOUK_003624"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFP9898994.1"
                     /translation="MSMPATLTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQLLTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5423..6886
                     /gene="citT"
                     /locus_tag="ACHOUK_003625"
     CDS             5423..6886
                     /gene="citT"
                     /locus_tag="ACHOUK_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFP9898995.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7000..7806
                     /gene="rna"
                     /locus_tag="ACHOUK_003626"
     CDS             7000..7806
                     /gene="rna"
                     /locus_tag="ACHOUK_003626"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFP9898996.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQVSIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8036..8446
                     /gene="rnk"
                     /locus_tag="ACHOUK_003627"
     CDS             8036..8446
                     /gene="rnk"
                     /locus_tag="ACHOUK_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFP9898997.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8584..8673
                     /gene="yldA"
                     /locus_tag="ACHOUK_003628"
     CDS             8584..8673
                     /gene="yldA"
                     /locus_tag="ACHOUK_003628"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFP9898998.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(8677..9915)
                     /gene="ybdR"
                     /locus_tag="ACHOUK_003629"
     CDS             complement(8677..9915)
                     /gene="ybdR"
                     /locus_tag="ACHOUK_003629"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HFP9898999.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10136..10564
                     /gene="uspG"
                     /locus_tag="ACHOUK_003630"
     CDS             10136..10564
                     /gene="uspG"
                     /locus_tag="ACHOUK_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFP9899000.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10685..12250)
                     /gene="ahpF"
                     /locus_tag="ACHOUK_003631"
     CDS             complement(10685..12250)
                     /gene="ahpF"
                     /locus_tag="ACHOUK_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFP9899001.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12379..12942)
                     /gene="ahpC"
                     /locus_tag="ACHOUK_003632"
     CDS             complement(12379..12942)
                     /gene="ahpC"
                     /locus_tag="ACHOUK_003632"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFP9899002.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13314..14060
                     /gene="dsbG"
                     /locus_tag="ACHOUK_003633"
     CDS             13314..14060
                     /gene="dsbG"
                     /locus_tag="ACHOUK_003633"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFP9899003.1"
                     /translation="MLKKILLLALLPAIAFAEKLPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
BASE COUNT         3391 a         3744 c         3561 g         3513 t
ORIGIN      
        1 cttaggatgt atagcgaaag gagaaaaaga tatacctcga tcacccccct ttctcccaag
       61 tgaaaataaa aggttatcag tttgcaacat tgaacaacat tcgttgcaaa tcgataacaa
      121 catgcacctt caggatacta tttattatgt tcggcaatga tattttcacc cgcgtaaaac
      181 gttcagaaaa taaaaaaatg gcggaaatcg cccaattcct gcatgaaaat gatttgagcg
      241 ttgacaccac agtcgaagta tttattaccg taacccgcga tgaaaagctt atcgcgtgcg
      301 gtggaattgc cggaaatatt attaaatgcg ttgctatcag tgaatccgtt cgcggtgaag
      361 gactggcgct gacattagcc actgaactga taaacctcgc ctatgagcgg cacagcacgc
      421 atctgtttat ttataccaaa accgaatacg aggcgctgtt ccgccagtgc ggtttttcca
      481 cgctgaccag cgtacccggc gtgatggtgc tgatggaaaa cagcgccacg cgactgaaac
      541 gctatgccga atcgctgaaa aaatttcgtc atccagggaa caagattggc tgcattgcga
      601 tgaacgccaa tccctttacg aatggtcacc gttatctgat tcaacaagct gcggcacagt
      661 gcgactggtt gcatctgttt ttagtcaaag aagattcttc acgcttcccc tatgaagacc
      721 ggctggatct ggtgttaaaa ggcaccgccg atattccacg cctgactgtg catcgcggct
      781 ccgaatacat catctcccgc gctacgttcc cttgctactt cattaaagaa cagagcgtca
      841 ttaaccattg ttacaccgaa attgatctga agattttccg tcagtacctc gctcccgcgc
      901 tgggtgtaac tcaccgcttt gtcggtactg aacccttttg tcgcgttacc gcccagtaca
      961 accaggatat gcgctactgg ctggaaacgc cgactatctc cgcagcgccc atcgaactgg
     1021 ttgaaattga gcggctgcgt taccaggaga tgccgatatc cgcttcccgg gtacgtcaac
     1081 tgctggcgaa aaacgatctc acggctatcg cgccgctggt ccctgcagtc acgctgcatt
     1141 atttgcagaa cctgcttgag cactcccgcc aggacgcggc agctcgtcaa aagacccccg
     1201 catgagaaac aggtgaaaaa tgaaaataaa ccagcccgcc gttgcaggca cccttgagtc
     1261 tggggatgtg atgatacgca tcgccccact cgatacgcag gatatcgacc tgcaaatcaa
     1321 tagcagcgtt gagaaacagt ttggcgatgc aattcgcacc accattctgg acgttctcgc
     1381 ccgctacaac gtgcgcggcg tacagctgaa tgtcgatgat aaaggcgcac tggactgcat
     1441 tttacgtgca cgactggaag ccctgctggc acgcgccagc ggtatcccgg ctctgccatg
     1501 ggaggattgc caatgatttc cgcttcgctg caacaacgta aaactcgcac ccgccgcagc
     1561 atgttgtttg tgcctggtgc caatgccgcg atggtcagca actccttcat ctacccggct
     1621 gatgccctga tgtttgacct cgaagactcc gtggcattac gtgaaaaaga caccgcccgc
     1681 cgcatggttt atcacgcgct gcaacatccg ctgtatcgcg atattgaaac cattgtgcgt
     1741 gtaaacgcgc tggattccga atggggtgtt aacgacctgg aagccgtcgt tcgcggtggt
     1801 gcggacgttg tacgtctgcc gaaaaccgat accgctcagg atgttctgga tatcgaaaaa
     1861 gagatcctgc gtatagaaaa agcctgtggt cgtgaacccg gcagcaccgg cctgctggcg
     1921 gcgattgaat ctccgctggg cattacccgc gcagtggaaa tcgctcacgc ttccgagcgt
     1981 ttgatcggta tcgccctcgg tgcagaagac tatgtgcgca acctgcgtac agaacgctcc
     2041 ccggaaggaa ctgaactgct gttcgcacgc tgttccattt tgcaggccgc gcgctctgcg
     2101 ggtattcagg cgttcgatac cgtctattcc gacgctaaca acgaagccgg attcctgcaa
     2161 gaagccgccc acatcaaaca gctgggcttt gacggcaaat cgctgatcaa cccgcgtcag
     2221 attgatctgc tgcacaacct ctacgcaccg acccagaaag aagtggatca cgcccgccgc
     2281 gtcgtagaag ccgctgaagc cgccgctcgc gaaggcctcg gcgtggtttc cctgaacggc
     2341 aagatggtgg acggtccggt tatcgatcgt gcccgtctgg tgctctcccg tgcagaactt
     2401 tccggcatcc gcgaagaata aggcaatcaa aatgacgcag aaaattgaac aatctcaacg
     2461 acaagaacgg gtagcggcct ggaatcgtcg cgctgaatgc gatcttgccg ctttccagaa
     2521 ctcaccaaag caaacctacc aggctgaaaa agcgcgcgat cgcaaactgt gcgccaacct
     2581 ggaagaagcg attcgtcgct ctggtttaca ggacggcatg acggtttcct tccatcacgc
     2641 tttccgtggc ggtgacctga ccgtcaatat ggtgatggac gtcatcgcga agatgggctt
     2701 taaaaacctg atcctggcgt ccagctccct gagtgattgc catgcgccgc tggtagaaca
     2761 cattcgccag ggcgtggtta cccgcattta tacctccggc ctgcgtggtc cactggcgga
     2821 agagatctcc cgtggtctgc tggcggaacc ggtgcaaatc cactctcacg gcggacgtgt
     2881 gcatctggta cagagcggcg aactgaatat cgacgtggct ttcctcggcg tcccgtcctg
     2941 tgatgaattc ggtaatgcca acggctacac cggtaaagcc tgctgcggct ccctcggcta
     3001 tgcaatggtt gatgccgaca acgcaaaaca ggttgtgatg cttaccgaag aactgctgcc
     3061 ttatccgcat aatccggcaa gcattgagca agatcaggtt gatttaatcg tcaaagttga
     3121 tcgcgttggc gatgctgcaa aaatcggcgc tggtgctacc cgtatgacca ctaacccgcg
     3181 cgaactgctt attgcccgta gcgctgcgga tgtgattgtc aactcaggct acttcaaaga
     3241 aggtttctcc atgcaaaccg gcaccggcgg cgcatcgctg gcggtaaccc ttttcctgga
     3301 agacaaaatg cgtagccgcg atattcgcgc cgacttcgcc ctcggtggta ttaccgcgac
     3361 gatggttgat ctgcacgaaa aaggtctgat ccgcaaactg ctggatgtac agagctttga
     3421 cagccacgct gcgcaatcgc tggcccgtaa ccccaatcac atcgaaatca gcgccaacca
     3481 gtacgctaac tggggttcga aaggtgcatc ggttgatcgt ctcgacgtgg tggtactgag
     3541 cgcgctggaa attgacaccc agttcaacgt taacgtgctg accggctctg acggcgtact
     3601 gcgtggtgct tccggtggtc actgcgatac cgcgattgcc tctgcgcttt ccatcatcgt
     3661 cgcgccgctg gtacgcggtc gtattccgac tctggtggat aacgtgctga cctgcatcac
     3721 cccgggctcc agtgtcgata ttctggtcac agaccacggt atcgcagtta acccggcacg
     3781 tccggaactg gcagaacgtc tgcaggaagc gggcattaaa gtggtttcca ttgagtggct
     3841 gcgcgaacgt gcgcgtctgc tgaccggtga accacagccg attgaattca ccgaccgcgt
     3901 cgttgccgtt gtgcgttacc gcgatggctc ggtgatcgat gttgtgcatc aggtgaagga
     3961 ataagccatg cacctgcttc ctgaactcgc cagccaccat gcggtgtcaa ttcccgagct
     4021 gctcgtcagc cgggatgaaa ggcaagcacg gcaacacgtc tggctcaagc gccatcctgt
     4081 tccactggtc tcctttaccg tggttgcgcc tgggccgatt aaagacagtg aggtcacacg
     4141 ccgaattttt aatcatggcg tgacagcctt gcgtgcctta gccgcaaaac agggctggca
     4201 aattcaggag caggctgcac tggtttccgc cagcgggccg gagggtatgt tgagcattgc
     4261 cgccccggct cgcgacctca agctcgccac cattgagctt gaacatagtc atcctctcgg
     4321 gcggttatgg gatatcgatg tcctgacgcc cgaaggcgaa attctctccc gccgcgacta
     4381 ttcactgccg cctcgccgct gcctgttgtg cgagcaaagc gcagccgtct gcgcgcgtgg
     4441 aaaaacccat caactgaccg atttactcaa ccgcatggag gcactgctga acgatgtcga
     4501 tgcctgcaac gttaactaaa accacaaagc ttgcgacgtc attaatcgat gagtacgccc
     4561 tgctgggctg gcgcgccatg ctgactgaag tcaatctgtc accgaaacca ggtctcgtgg
     4621 atcgcattaa ctgcggtgcg cataaagata tggcgctgga agatttccac cgcagcgcgc
     4681 tggcgattca gggctggcta ccccgtttca ttgaatttgg tgcctgtagt gcggaaatgg
     4741 caccagaagc ggtactccac ggattacgcc caattggtat ggcttgcgaa ggtgatatgt
     4801 tccgcgccac cgcgggcgta aacacgcata agggcagcat tttttcttta gggctactgt
     4861 gtgcggcaat tggccgtttg cttcagctca accagttgct aacgccaaca accgtttgtt
     4921 ctacggcggc aagtttctgc cgtggcctga ccgatcgcga actgcgtacc aataattcac
     4981 aactgacggc aggtcaacgg ttgtaccagc agcttggcct taccggcgca cgcggtgaag
     5041 ccgaagcggg ttatccactg gtgatcaacc acgccttgcc gcattacctc actctgctgg
     5101 atcaggggtt agatcctgaa ctggcattgc tcgatacctt gctcctactg atggcgatca
     5161 acggcgatac caacgttgct tcgcgcggtg gcgagggggg cctgcgctgg ctacagcgcg
     5221 aggcgcaaac attattgcaa aaagggggca ttcgaacccc cgcagatctc gattatctcc
     5281 ggcagttcga cagggagtgt atcgaacgaa atctcagtcc aggcggcagt gccgacctgc
     5341 tgatccttac ctggttttta gcacagattt aattatttaa gaacttgata aatttggaaa
     5401 tattaatttt cggagaaccc gtatgtcttt agcaaaagat aatatatgga aactattggc
     5461 cccactggtg gtgatgggtg tcatgtttct tatccctgtc cccgacggta tgccgccgca
     5521 ggcatggcat tacttcgctg tgtttgtggc aatgattgtc ggcatgatcc tcgagccaat
     5581 tccggcaaca gcgatcagtt ttattgcggt tactatttgc gttattggca gtaattacct
     5641 gctctttgat gccaaagaat tagctgaccc agcgtttaat gcgcaaaaac aggcgctgaa
     5701 atggggcctg gctggttttt ccagcactac ggtatggctg gtatttggcg catttatttt
     5761 tgcattaggg tatgaagttt ccgggttagg tcgtcgcatt gcccttttcc tggtgaaatt
     5821 catgggcaaa cgcacgctga cgttgggtta tgcgattgtc attatcgaca ttctgctggc
     5881 accgtttaca ccgtccaaca ccgcgcgtac cgggggtacg gtttttccgg tcattaaaaa
     5941 cctgccgccg ttgtttaaat cattcccgaa cgatccgtcc gcgcgtcgta ttggcggcta
     6001 tttgatgtgg atgatggtca ttagtaccag tctgagttcg tccatgtttg tcaccggtgc
     6061 ggcaccaaac gtgctgggtc tggagttcgt cagcaaaatt gccggtatcc agattagctg
     6121 gttgcagtgg ttcctctgct tcctgccggt tggggttatc ttgcttatca ttgcgccgtg
     6181 gctttcctac gtgctgtaca aaccggaaat cacacacagt gaagaagtgg caacctgggc
     6241 gggtgatgaa ctaaaaacca tgggtgcgct gacacgcaga gagtggacgc tgattggcct
     6301 tgtattgctc agcttaggtt tgtgggtatt tggcagtgaa gtcattaatg ctactgcggt
     6361 tggtctgctg gcagtttcgc taatgctggc tctgcacgtt gtgccgtgga aagacattac
     6421 ccgctataac agcgcatgga acacgctggt caacctggca actctggttg tgatggctaa
     6481 cggcctgact cgttctggtt ttattgactg gttcgccggt accatgagta cgcacctgga
     6541 aggattctca ccaaacgcaa cggtgattgt actggttctg gtgttctact ttgcacacta
     6601 cctgtttgcc agcctgtctg cgcacaccgc aaccatgctg ccggttattc tggccgtcgg
     6661 taaaggtatt ccgggcgtac caatggaaca actgtgtatc ctgctggtgc tgtctatcgg
     6721 tatcatgggc tgtctgacgc cgtatgcaac cggtcctggg gtgattattt acggctgtgg
     6781 ctatgtgaaa tcaaaagatt actggcgtct tggcgcaatc ttcggggtga tttacatctc
     6841 tatgttgctg ttggttggct ggccgattct cgccatgtgg aactaatacg ttcgccaaac
     6901 gctgaataaa tcatcacgcc cgccaggtta gcccacctgg cgggcttttt tatgatttaa
     6961 tagatagtat tcagtgtatg tatgagttcc acacccatta tgaaagcatt ctggcgtaac
     7021 gccgcgttgc tcgcggtttc tctgcttccc ttctcttctg ccaacgccgt agcgttgcag
     7081 gcaaaacagt atggcgattt tgatcgctat gtcctggccc tctcctggca aaccggattt
     7141 tgccagagtc aacacgatcg aaatcgtaac gaacgagatg aatgtcgcct gcaaaccgaa
     7201 acgaccaaca aagctgattt tctgaccgta catggtctgt ggccaggatt gcctaaatcg
     7261 gttgctgccc gtggtgttga tgaacgccgc tggatgcgct tcggttgcgc tactcgccca
     7321 atcccgaatc taccagaagc gcgcgccagc cggatgtgtt catcgccgga aaccggatta
     7381 tcactggaaa cggccgctaa actaagtgaa gtcatgccag gagctggcgg acgttcctgc
     7441 ctggaacgct acgaatatgc caaacacggt gcctgctttg gttttgatcc ggacgcatac
     7501 ttcggtacga tggtacgcct gaatcaagag ataaaagaga gcgaagccgg aaaattcctt
     7561 gcggataatt acggtaaaac agtgagccgc cgtgactttg acgccgcctt tgccaaaagc
     7621 tggggaaaag agaacgtgaa agcagttaag ctaacgtgcc agggtaaccc tgcgtatttg
     7681 actgaaattc aggtctcgat caaagctgac gccatcaacg ctccgctttc tgcaaactca
     7741 ttcttgccac aaccacaccc aggtaactgt ggcaaaacct ttgtgattga taaagcgggt
     7801 tattaatcct ctgatttaca aaacacgcta attcgtcttt cataaaccat gacgattaag
     7861 aattatacat aatgcgctgc attgaggaat tccctgaatg tgacgcaaat cacttcaaag
     7921 agtgagcaac agtcagtatc atcatgtgag ttaaaccctc gccgcctgac ggtgagggtt
     7981 ttcttttggg tatgtttatc tgcgacactc gcagtaccga cgacatggag taaaaatgtc
     8041 cagaccaact atcatcatta acgacctgga tgccgaacgc atcgatattc tgctggagca
     8101 acccgcctat gctggtttgc caatcgccga cgcgttaaac gcagagttgg atcgcgccca
     8161 aatgtgttcg ccagaagaga tgccacacga cgtggtgaca atgaacagcc gggttaaatt
     8221 ccgcaatctt agcgatggcg aagtgcgtgt gcgcacgctg gtgtatccgg caaaaatgac
     8281 cgatagcaat actcagcttt ccgttatggc tccggtaggt gccgcactgc tgggactgcg
     8341 cgttggcgat tccattcact gggaacttcc gggcggcgtt gcaacccacc ttgaagtgct
     8401 ggaactcgaa taccagccag aagctgctgg cgactacctg ctttaatccc atctgtacaa
     8461 gcgataactc gctttgccca gaggatgcac tcgcatcctc tttatgcaaa atcagcaatt
     8521 tttcctggtt ctcactgttc acttttgctg caaaattatt ccgtttcgtt tgtgggaata
     8581 accatggcag aagcatttta catcctgata ggatttctga ttatggcggc gattatcgtc
     8641 atggctgtcc tttacctcga aaaccactcc tgacgctcat attgttcccc ccggcatcgc
     8701 attcaccaga cctgaaaccg ccttctgcgc cgcctctgcg ctttgtgcac cgggtaccag
     8761 aatcacctta cggcactcct cttcacgctt ttcaaagatc tcatatcccc gggcggcctc
     8821 ttcaaacggc atatagtggg taacaatttc ttctggtttc agtaatcctt tctcaattaa
     8881 cggtaataat tctcccagcc atgcgtgaac gtgggtctgt cccattttaa acgtcaaccc
     8941 tttatcaaag gcgtcgccaa acaggaaacc gtgaataaat ccagcgtaga cgcccggtac
     9001 gctaacaatg ccgccacgcc tgaccgccgc aatacactga cgcaacgctt taccgctgct
     9061 gccctccagt ttcaggttag tcagcaccgt ttccgtggtg ctgcctttcg cttcaaaacc
     9121 gacggcgtct attactgcat ccacgccccg gtgacctgcc gtttgttcaa taattgactg
     9181 tgccggatcg ctatcttcat cgaaattaat cgggatcgcg ccgtagcggt cagcggcgaa
     9241 acgcaagcgg tagggatgat gatcaacaac aaaaatctgt tccgcaccga gcaaccgtgc
     9301 acaggcgatt gtcaacaatc ccacaggacc ggcaccatag acagcaacgc ttgaaccttg
     9361 ttggatctgc gcattttttg ctgcctgcca tgccgttggc agaatatcag aaaggaaaag
     9421 ggctttatca tctgaaagca aaggcggtac tttaaacggc cccacattcc ctttagggac
     9481 acggacatac tccgcctgtc caccaggaac gccaccatac aggtgactat aaccaaacaa
     9541 tgccgcggga gctggtatct gttttttatt gagcgcagcg cctttacccg cattggtatt
     9601 ttcgcaggcg gcatattgct gcagtcgaca gaaaaaacag tcgccacaag caatgacgaa
     9661 cggaattacc acccggtcgc ctttttgcaa attttttaca tcctttccgg tttcaactac
     9721 ttcccccata aattcatgac caaaaatatc gccatgttta acctggggta ttttgcctcg
     9781 ataaagatgg aggtcagagc cacagatcgc cgttgccgtt atacgcagaa taatatcatc
     9841 tgcctgttca atgcccggat cgggaacatt ttctacctga acgtgatgtg ggccgtgata
     9901 agtcaatgct ttcatgcaac ctccagagac agttcaaaaa agagtcagtc tttaagggta
     9961 gctatcacag cgggaaaacg ggcggtaccg caggttttgg gagaattcct ggtgttcagt
    10021 gagaatatat tcatcacaga aacagttcgt aaggaaaaag aaccagacaa atatcaggga
    10081 taaatattat aatcatggcg aatgatttgt tcatgattaa cagggagaaa gggttatgta
    10141 taagacaatc attatgccag ttgatgtatt tgaaatggaa ttgagcgaca aagctgttcg
    10201 ccacgctgaa ttcctcgccc aggatgacgg agttattcat ctacttcacg tactacccgg
    10261 gtcagccagc ctgagcctgc accgttttgc cgctgatgtg cgtcgttttg aagagcatct
    10321 gcaacatgaa gcagaagaac gtttgcaaac gatggtcagc cacttcacca tcgatccttc
    10381 ccgcattaaa caacatgtcc gttttggtag cgtgcgggat gaagtcaatg agttagcaga
    10441 agaactgggg gctgatgttg tagttattgg ttctcgcaac ccatcgattt cgacccatct
    10501 gttaggttct aacgcctcga gcgtgatccg ccacgccaat ctgcctgtac tggtcgttcg
    10561 ctaacagata aaagtattta tgccgccctg cctggaacat cccggcaggg ctttttaatg
    10621 ccataaaaaa gccgccaggt ttgaccccgg cggctaagca attgcaggtg aatcttactt
    10681 cttcttatgc agttttggtg cgaatcaggt agtcaaaagc actcagagag gctttggcac
    10741 cttcgccagt ggcgatgatg atctgcttgt acggaaccgt cgtacagtca cccgctgcga
    10801 acacgccttt cacgttggtt tcgcatttcg catcaatgat aatctcgccc atgcggttac
    10861 gttcgactgc gccttcgagc cagttggtgt tcggcagcag accaatctgc acgaaaatac
    10921 cggccagttc gatgttgtga atatcgccgc tgacacgatc tcgatattcc agaccaacga
    10981 ctttgctgcc gtcgcctttc acttccgtgg tttgcgcatt cagaataatg tcgacgtttt
    11041 tcaggctgcg cagtttgtcc tgcagaacct ggtcggcttt catttctggc gcaaattcca
    11101 gcagcgttac gtgctcaacg atacccgcca ggtcaattgc cgcttccacg ccggagttac
    11161 cgccgccgat aaccgctacg cgtttacctt taaacagcgg gccgtcgcag tgcgggcagt
    11221 aggtcacgcc tttggtgcga tactgatctt cgcccggaac gttcatgttg cgccattttg
    11281 caccggtcgc cacgataatg ctgcgtgctt tcagtaccgc gccagaagct gtttcaatct
    11341 gatgcagacc accttcaact gctgctggga tcagtttgct ggcgctctgg ctgtcgatca
    11401 catcaacgtc gtattcatca acgtgaactt tcagtgcgcc tgccagcttc tgcccttcag
    11461 tcttcggtac agaaatgtag ttttcgatat caacggtatc gaggatctga ccaccaaaac
    11521 gttcgcccat cagaccggta cggatgcctt tacgtgcgga gtaaattgct gccgctgcac
    11581 ccgccgggcc ggaaccgacg attaatacgt cataagcatc acgcttgttc agctcttctg
    11641 ccgcacgttt ttccgcgccc gtatcaattt tggcaacgat ttcagtcaaa gtcatgcggc
    11701 cctgaccaaa ctctttcccg ttcacgaaca ctgccggaac gcccatcacg ttacgatcag
    11761 tgatctcgtt ctggaaggtg ccgccgtcaa ttgcagtgtg cttgatgcgc gggttcaata
    11821 cgctcatcag gttcagcgcc tgtaccacgt ccgggcagtt atggcaagag agcgagtaat
    11881 aggtttcgaa ttcaaaatca ccgtcaatat ggcgaatctg ctccagcaga gactgcgctt
    11941 ctttcgacgg atgaccaccg gtccacagta acgccagtac cagcgaggtg aactcgtggc
    12001 ccagcgggga gcctgcaaaa cgtggcccct ggttagaacc tgggttggtg atcaggaaag
    12061 acggcttacg caccggcaag ctgttatctt ctttaaaggt gactttgtct gacagttctg
    12121 cgatttcagc caacagttcc ttgatttctg ccgatttagc gctgtcatcc agcgtggcaa
    12181 ttaactcaac aggcttggtc aatttctcaa ggtaagcctt gagttgagtt ttcatatttg
    12241 tgtcgagcat gtttatctcc tgggctttaa acatcatcat gcaagcggcc tctgcggcta
    12301 cctggatgca gcttgcatca tggtgcgggg aagaaaatcg ggtgccgcag cacccgatta
    12361 attacgcgat tgcggaactt agattttacc aaccaggtcc agagacggag ccagagttgc
    12421 ttcaccttct ttccatttag ccgggcaaac ttcacctggg tgagaagcta cgtactgtgc
    12481 tgctttgatt ttacgcagca ggtcagacgc gtcacggcca atgccttcag cggtaacttc
    12541 gattgcctgg atgataccct gcgggtcaac aacgaaggtc gcacggtcag ccagaccttc
    12601 atcttcacgc atgttgtcga agttacgggt cagggcgcca gtcgggtcgc cgatcatcgc
    12661 atatttgatt ttagcgatgg tttcagagct gctgtgccat gctttgtggg tgaagtgagt
    12721 atcggtagat actgcgtata cgtctacgcc cagtttctgc agttcttcgt agtggtcagc
    12781 aacgtcaccc agttcggtcg ggcatacgaa agtaaagtca gccgggtaga agaagaagac
    12841 gctccagcgg ccttcggtat ctttttcggt gatttcgatg aattcgccgt ttttgaatgc
    12901 ctggttttta aaaggtttaa ttttggtgtt aatcaaggac atctatactt cctccgtgtt
    12961 ttcgatgaga tgtaaggtaa ccaattttta ccgattcggc taatgcgttt ccattatcaa
    13021 atcgataagt tttaccttac aaccttcgta agacaacttt gtgaactttg caccgccagc
    13081 ggcgataaaa agtaaaatgg ccccctttcc aggtgaccgt gtaatctgaa ctaccctttg
    13141 gtatcttcag cgccagtctg caggctgcgt tgttgctctt acaaatgcaa caacgacatg
    13201 gattacaaca ccctcataaa caaagggcaa tcacctgatc taagctctta cctatgacaa
    13261 tgataggtta tgccttttac tcgacttttg cactgactga aaaggacaaa ttaatgttaa
    13321 aaaagatact tttactggct ctgcttcctg caatcgcctt cgcagagaaa cttcctgctc
    13381 cagtaaaagc gattgaaaaa cagggcatta caatcatcaa aacgttcgat gcccccggag
    13441 gaatgaaagg ttatctcgga aagtatcagg atatgggcgt caccatctac ctgactccag
    13501 atggtaagca cgctatctct ggttacatgt acaacgagaa aggtgaaaac ctgagtaaca
    13561 cacttatcga aaaagaaatt tacgcaccag ccggacgcga aatgtggcaa cggatggaac
    13621 aatcccactg gctcctcgac ggtaaaaaag atgcgccggt cattgtctac gtcttcgccg
    13681 atccgttctg cccatattgt aaacagttct ggcagcaggc gcgcccgtgg gtagattctg
    13741 gcaaagtaca gttgagaaca ttgttggttg gggttatcaa accagaaagt ccggcgacag
    13801 ccgcggcaat tcttgcctcc aaagatcccg caaaaacctg gcaagaatat gaagcctctg
    13861 gtggcaagct taagctaaac gtgcctgcaa acgtaagtac agagcaaatg aaagtgttaa
    13921 gcgacaacga gaaactcatg gacgatctgg gggcaaatgt cactccggct atctattaca
    13981 tgagtaagga aaatacgctg caacaggccg tggggttgcc cgatcagaaa acgcttaata
    14041 tcattatggg aaataaataa aaggtaattc atggatggtt gaactatatc cctgcttttc
    14101 aaccatccta aaaaagcatg aattgctcgc tctgccctaa gtggctgtgc tattttagaa
    14161 aaacttaatc ctgagttaag caaaaaaaaa cataatcaat aaaaatata
//
LOCUS       DAZKIP010000111        13945 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZKIP010000111 DAZKIP010000000
VERSION     DAZKIP010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.112"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            137..646
                     /gene="ybgA"
                     /locus_tag="ACHOUK_003634"
     CDS             137..646
                     /gene="ybgA"
                     /locus_tag="ACHOUK_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFP9899004.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIESTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPYAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            643..2061
                     /gene="phrB"
                     /locus_tag="ACHOUK_003635"
     CDS             643..2061
                     /gene="phrB"
                     /locus_tag="ACHOUK_003635"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFP9899005.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARLLALYIATPRQWAA
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFAASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVQVERTLRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLNEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRQWLPELRNVPGKSVHEPWKWAEKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(2211..3692)
                     /gene="dtpD"
                     /locus_tag="ACHOUK_003636"
     CDS             complement(2211..3692)
                     /gene="dtpD"
                     /locus_tag="ACHOUK_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFP9899006.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELRLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGVVLMIWLYQALKFRNRALALES"
     gene            3963..4706
                     /gene="ybgI"
                     /locus_tag="ACHOUK_003637"
     CDS             3963..4706
                     /gene="ybgI"
                     /locus_tag="ACHOUK_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFP9899007.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            4729..5385
                     /gene="pxpB"
                     /locus_tag="ACHOUK_003638"
     CDS             4729..5385
                     /gene="pxpB"
                     /locus_tag="ACHOUK_003638"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFP9899008.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            5379..6311
                     /gene="pxpC"
                     /locus_tag="ACHOUK_003639"
     CDS             5379..6311
                     /gene="pxpC"
                     /locus_tag="ACHOUK_003639"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFP9899009.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKAKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            6301..7035
                     /gene="pxpA"
                     /locus_tag="ACHOUK_003640"
     CDS             6301..7035
                     /gene="pxpA"
                     /locus_tag="ACHOUK_003640"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFP9899010.1"
                     /translation="MKIDLNADLGEGCTSDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRKNFGRSAMQLPPETVYAQTLYQIGALATITRAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQSGALIEDEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSAFAEKGIVVAA"
     gene            7071..7862
                     /gene="nei"
                     /locus_tag="ACHOUK_003641"
     CDS             7071..7862
                     /gene="nei"
                     /locus_tag="ACHOUK_003641"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFP9899011.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEESQTTRVLRVKLQTADKSIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(7859..8905)
                     /gene="abrB"
                     /locus_tag="ACHOUK_003642"
     CDS             complement(7859..8905)
                     /gene="abrB"
                     /locus_tag="ACHOUK_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFP9899012.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLGLHLPAALLLGPMIAGIIFSMRG
                     VTLQLPRSAFLAAQAILGCMIAQNLTGSILTTLEVNWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQHIVWFPPVSINLLLTILLAVVAGTVGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRTA"
     gene            complement(9054..10115)
                     /gene="ybgO"
                     /locus_tag="ACHOUK_003643"
     CDS             complement(9054..10115)
                     /gene="ybgO"
                     /locus_tag="ACHOUK_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9899013.1"
                     /translation="MRFAKGVLLAICLIFLPLKAALALNCYFGTANGAVEKSEAIMPF
                     AVPANSKPGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGIQDPYYQLGVTY
                     EGVDYDASLGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKLCSGDPTVMHKSRTFVA
                     RMRLYVKVRAMPPHDYQSKLSDYIVVQFDGAGSVNEDPTAKNLKYHITGLENIRVLDC
                     SVNFAISPETQVVDFGRFNVLDIRRHTMSQQFKITTTKSQNDQCTDGFKVSSSFYTDE
                     TLIDEDKSLLIGNGLKLRLLDENASPYTFNKYSEYADFTSDLLVYEKTYTAELSSTPG
                     TPIDVGPFDTVVLFKINYN"
     gene            complement(10112..10843)
                     /locus_tag="ACHOUK_003644"
     CDS             complement(10112..10843)
                     /locus_tag="ACHOUK_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP9899014.1"
                     /translation="MTFVKGFPLILLVASMCSHGAVQPDRTRIIFNSKDKATSLRVEN
                     RSDKLPYLAYSWIENEKGEKSDEFLVALPPIQRLEPKATSQVRIMKQAATAKLPTDRE
                     SLFYYNLREIPPVPEGSEGHAILQVAVQSRIKLFWRPAALRKKMGDHVEQQLQVSQQN
                     NQLTLKTPTGYYLTIAYLGRDEKGVLPGFKSTMVAPFSSVTTSTGSYSGKQFYLGYMD
                     DYGALRMNTLSCQRQCRLQPVENKK"
     gene            complement(10858..13317)
                     /locus_tag="ACHOUK_003645"
     CDS             complement(10858..13317)
                     /locus_tag="ACHOUK_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP9899015.1"
                     /translation="MDAVNIYRLSVLSCLAMVTPPALTAEFNLNVLDKSIRDSVDISL
                     LNQKGVVAPGDYFVSVTVNNNKISNGQQIRWQKSGDKIIPCINESLIELFGLKSDFRK
                     KLPAIKECVDFSVFPEIIFTFDQANQQLNITIPQAWLAWHSENWTPPSTWNNGIPGFL
                     MDYNLFASTYRPQSGSSSNNLNAYGTTGLNAGAWRLRSDYQLSQSDSGDNREQSGAIS
                     RTYLFRPLPQIGSRLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISHSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVSEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGRPRSSMSHHTEDETFISHEVSWGMLSNTSLYGGMLLAGDD
                     YRSGALGIGQNMLWMGALSFDVTWADSHFDTQQDEQGYSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYIDHKYNDADAQDEKQTISLSFGQPITLLNLNLYANILHQSWWN
                     ADTSTTANITVGFNVDIGDWKDISVSTSFNTTHYEDKDRDNQIYFSISLPIGESGRLG
                     YDMQNNSNTTTHRMSWNDTLDERNSWGMSAGIQSDRPDNGAQVSGNYQHLSSAGEWDL
                     TGTYAANDYTSASASWSGSFTATQHGAAFHRRSSTNEPRLMVSTDGVGDIPIQGNIDY
                     TNRFGIAVVPFVSSYQPTTVAVNMNDLPDGVTVSENVVKETWTEGAIGFKSLASRAGK
                     DLNVIISDANGHFPPLGADVRQAEGGVSVGMVGENGHAWLSGVDENQQFTVHWGDQKT
                     CAIHLPEHLEDVTKRLILPCH"
     gene            complement(13367..13933)
                     /locus_tag="ACHOUK_003646"
     CDS             complement(13367..13933)
                     /locus_tag="ACHOUK_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9899016.1"
                     /translation="MFKGQKTLAALAISLLFASPVFAADEGSGEIHFKGEVIEAPCEI
                     HPDDLDMNIDLGEVTTSHINREHHSEKKPVNIRLINCDIAGWDDGKGGVVSKVGVTFD
                     STAKTTGATPLLSNISAGEATGVGVRLMNKDESFVTLGTEAPTIDLLASSTEQTLNFF
                     AWMEQMDNAVSVTAGAVTANATYVLDYQ"
BASE COUNT         3379 a         3453 c         3795 g         3318 t
ORIGIN      
        1 attcgctgct ttgatgatga tgtaaaatga ttgtttttga actggatata acagcttggt
       61 agggaagaaa gcataacccc acaaaattca tttaccatcg tctacactta ctcctgtaaa
      121 ccgttcagga gcagtaatga atcagcaacg atttgatgac agcactctaa tccgtatctt
      181 cgcgcttcat gagttacatc gactgaaaga acatggctta acgcgcgggg cgcttctcga
      241 ttatcacagc cgctataaac tcgtatttct ggctcattcc cagccggaat accgcaaact
      301 tggcccgttc gtggctgata ttcaccagtg gcaaaatctg gatgattttt acaaccagta
      361 ccgccaacgc gtaattgttt tgctttctca tcccgccaac ccgcgcgatc acaccaacgt
      421 tttgatgcac gttcagggtt attttcgccc gcatattgag tccacagaac gccagcagct
      481 ggctgcgctt atcgacagtt atcgccgtgg cgagcaaccg cttcttgcgc cgctgatgcg
      541 tatcaaacac tatatggcgc tttatcctta cgcctggctt tcagggcagc gttatttcga
      601 actttggccg cgtgtgatta acttgcgcca ttcaggagtt ttatgactac ccatctggtc
      661 tggtttcgcc aggatttacg tctgcatgat aatctcgcgc tggctgccgc ctgccgcaat
      721 tcatctgcac gcctgctggc gttgtatatc gctacaccac gccagtgggc ggcgcataat
      781 atgtcgccgc gtcaggctga acttatcaat gctcaactga acgggctaca aatcgcgctg
      841 gcggaaaaag gcattccttt attgttccgt gaagtggatg actttgccgc cagcgtcgaa
      901 atagttaaac aggtgtgcgc ggaaaacagc gttactcatc ttttttataa ctatcagtat
      961 gaggtgaatg agcgggcgcg ggatgtgcag gttgagagaa ctctgcgtaa cgtggtgtgt
     1021 gaaggatttg atgacagcgt gatccttccg cctggcgcgg tgatgaccgg caatcatgag
     1081 atgtacaaag tctttacgcc ttttaagaat gcctggctga aacggctgcg ggaagggatg
     1141 ccggagtgcg tcgctgcacc aaaagttcgt agtagcggat cgataaagcc cgcgccatcc
     1201 attacgctga attatcctcg tcagtctttc gatactgcgc atttcccggt ggaagaaaaa
     1261 gcggcgattg cgcaattacg ccagttttgc cagaacggtg ccggagaata tgagcaacaa
     1321 cgagattttc cggcagtgga aggcaccagt cgtttgtccg ccagcctggc aacgggcggg
     1381 ttatcgcctc gccagtgttt gcatcgcttg ttggcggaac agccgcaggc gctggacggt
     1441 ggggccggta gtgtctggct taatgagctg atctggcgcg agttctaccg tcatctgatg
     1501 acgtattacc cctcgttgtg taaacattgt ccgtttattg cctggacgga tcgtgtgcag
     1561 tggcagagca atcccgcaca tttacaggcc tggcaggaag gcaaaacggg atacccgatt
     1621 gtcgatgctg ccatgcgtca gcttaacagc actggctgga tgcataacag gctacggatg
     1681 attacagcca gttttctggt gaaagatttg ttgatcgact ggcgcgaagg cgagcgatat
     1741 ttcatgtcgc agctgattga tggtgatttg gcagccaata acggtggctg gcagtgggcg
     1801 gcttctacgg gtactgatgc agcgccgtat tttcgtattt tcaacccgac aacccagggc
     1861 gagaaatttg accgcgaagg cgagtttatc cgccagtggc taccggaact gcgcaatgta
     1921 ccggggaaat cggtgcatga gccgtggaag tgggctgaga aagcaggtgt gaagctggat
     1981 tatccgcaac cgatagtcga gcacaaagag gcgagagtac aaacgttggc agcgtatgag
     2041 gcggcgcgga aggggaaata acagacgcgt caggcaatcg agcccagatg ccggatgcgg
     2101 cgtgaacgcc ttatccagcc tacaaatttg tgcaaattca ataaattgca ggaaatacgt
     2161 aggcctgata agacgcgtca agcgtcgcat caggcatcgg tgctggccta ttaagactcc
     2221 agcgccagcg cgcggtttct gaatttcagc gcctgataca gccaaatcat cagtaccact
     2281 cctacacacg ccagtgcgcc ccaggtaatt tgatcaaata cttcaatata tgcattgatg
     2341 gagtagttga tcgccccgga agcatcaaac gaagcctgcg atgtctgatc ggcaatcacg
     2401 cccgccagat agttcgcaat cgcgccggaa agcagcatat aaatgccggt taatacgccg
     2461 gtcacaccgg ggatttcaat gcgcgtaatc tgcgacatag caaccgggtc gataaacagt
     2521 tccgcaaagc ccatcaccgc caggcctaat accatcagtg gcagagaaga gtgaccatac
     2581 attgcggacc agcgggcgct taaggtcaga atgcagaatc cggcgctcat caggccaagg
     2641 ccaagagcaa attttcccca gatgcgcacg gtacgattac ccgcgacact ctcttttacc
     2701 acccacgcca ggaacacacc gcacagcata actgcgaagg cattaatcga ctggaacatc
     2761 gcggtcggaa cggtataacc aaacatatcg cgattaacga agcggtcgat ataaaggcta
     2821 atcgagctac cgccctgttg tgcgaaggcc cagaacaaca tactgaagaa ggtgagcgtc
     2881 acaatcagcc tcagctcctt ccgctgtttc tggttttctg ctttgcgata aatttttgcc
     2941 agtacaccca ggccaatgat agtcgcgaca atcaaggcgt ataccgacca ttctttccag
     3001 aacagtacgg taatcagcgc aggcgttgcc accagcagaa ccagcagcca tccccagttc
     3061 ggcagcagaa agtttgtcgc acgcagtact tttttgttaa cgccgcgggt atgtgtgaaa
     3121 tgacgattgc cacataagaa aataaccaga cccgcgatca tacccaccgc cgccaggcca
     3181 aagcccatcg cccaactgta ctcttcctgg gcgtagccac aggcaatagg tgcgataata
     3241 gaccccacgt tacccgctgc atacatcagc gagaaaccgc catcacgacg cggatcggtt
     3301 ggctcataca gctcgccgag caggcaactg acattggatt taaacagacc atagccacaa
     3361 acgattatcg ccagggacag atagaggaat gacggatgga tctcacttgc acccaacacc
     3421 acatgaccga tcgccatcaa taacgccccc agcatcaccg ccatgcgatt gccgagaact
     3481 ttatccgcca aaaagccacc gaggattggc gtgacataca ccagcgaaca gtaggcgcta
     3541 aataactcgt aggcgtgagt atcgttgtat tttagttgat tggtgagata gagaatcagc
     3601 agggcacgca tgccgtaaaa gctaaaatat tcccagattt gcagcgcaac gacatagtaa
     3661 atagcgcgcg gttgtgatgc gtgtttattc ataataattc cacaattgac ggcaacgtca
     3721 cgtaatagtt gcgcggcgtg cttgtgtttc cttaaagtta aaactttgat acagatctga
     3781 ttatttatgc aatatgctgt ctgattgcat aaatatacat tagctgaagc gtgatgatat
     3841 aaacaatttg cgtcttttgg cgtcgggttt gtttcttaat atgtcaaaag attggctatc
     3901 gacgaacagg cgaggtaacg ctatgttaac ggtgtagcta tcagaatacg gaaggcgcga
     3961 agatgaaaaa caccgaactg gaacaactga ttaacgaaaa actgaacagc gcggcgatta
     4021 gtgattacgc gccgaatggt ttgcaggtgg aaggcaaaga gacggtgcaa aaaattgtta
     4081 ccggtgtcac tgccagccag gctttgctcg atgaggcagt gcgtctgggc gcggatgcgg
     4141 tcattgtgca tcatggctac ttctggaaag gcgaatctcc ggtcattcgc ggcatgaagc
     4201 gtaaccgttt aaaaacgttg ctggcgaatg atatcaacct gtatggctgg catttgccgc
     4261 ttgacgcaca tcctgagctg ggcaataacg cacagctggc ggcgttactg gggatcacgg
     4321 tcatgggcga aattgagccg ttggtgccgt ggggcgaact gaccatgcct gtgccgggac
     4381 tggagctggc ttcctggatt gaagcgcgtc tgggacgtaa gccgttatgg tgtggcgata
     4441 ccggacctga ggtggtccag cgcgtcgcct ggtgcacggg cggcgggcaa agttttatcg
     4501 atagcgccgc gcgttttggc gtggatgctt ttattactgg cgaagtttct gaacagacca
     4561 ttcattcagc ccgcgagcag ggattgcatt tttatgctgc aggtcaccat gccactgaac
     4621 gtggtggtat tcgcgcattg agcgagtggc tgaatgaaaa taccgatctt gatgtgacct
     4681 ttattgatat tcctaatcct gcataacgaa taatcagagg gatcgaaagt gcaacgagcg
     4741 cgttgttatc tgataggtga aacggcggta gtgctggaac tggaaccgcc ggtgacgctg
     4801 gctagccaga aacggatctg gcgactggcg cagcgtctgg tggatatgcc gaatgtggtt
     4861 gaagccattc ccggcatgaa caatatcacg gtgattttgc gtaatcctga gtcgctggcg
     4921 ctggatgcca tagagcgttt gcaacgctgg tgggaggaga gcgaggccct ggaacctgag
     4981 tctcgcttta ttgaaattcc ggtggtttac ggtggtgcag gcggaccgga tttggcggtg
     5041 gtcgcggcgc attgcgggtt gagcgaaaaa caggttgttg aattgcactc ctccgtggaa
     5101 tacgtggtct ggtttttagg ttttcaaccg ggcttcccgt atctcgggag tttgccggaa
     5161 caactacaca cgccacggcg cgctgaaccg cgcttgctcg ttccggcagg ttctgtcggg
     5221 atcggcgggc cgcagactgg tgtttatccg ctggcaacgc cgggtggctg gcagttgatt
     5281 ggtcatacct cactcagcct gtttgatccg gcgcgtgacg aacccatctt attacgtccg
     5341 ggagacagcg tgcgctttgt accgcaaaag gagggagtat gctgaagatt attcgtgcgg
     5401 gcatgtatac cactgtgcaa gatggcggtc gtcacggttt tcgccagtcg ggtatcagcc
     5461 actgcggcgc actggatatg cctgcgttac gcattgctaa cctgctggtg ggtaatgatg
     5521 ccaatgcccc cgcgctggag atcacgctcg gtcagttaac ggttgagttc gaaactgatg
     5581 ggtggtttgc tctgacgggt gccggttgcg aagcgcggct ggatgataat gccgtctgga
     5641 ccggctggcg attgccgatg aaagcaggcc agcgtttaac gcttaaacgc ccgcagcacg
     5701 ggatgcgcag ttatctggct gtcgcgggtg gtattgatgt tccgccggta atggggtcat
     5761 gcagcaccga tctcaaagtg gggattggcg ggctggaagg ccgtttactg aaggatggtg
     5821 accgactccc gattggcaaa gcgaagcgtg attttatgga agcgcagggc gttaaacagc
     5881 tgctgtgggg caaccgcatt cgcgccttgc cggggccgga atatcatgag ttcgatcgcg
     5941 cctcgcagga tgcattctgg cgttcgccct ggcagcttag ctcgcaaagt aaccgcatgg
     6001 gctatcgctt acaggggcaa attttaaaac gcaccaccga tcgcgaactg ttatctcacg
     6061 gtttgttacc gggcgtggtg caggtgccgc ataacgggca gcccattgtg ttgatgaacg
     6121 acgcacagac caccggtggt tacccgcgta ttgcctgtat cattgaggct gatatgtacc
     6181 atctggcgca aattccgctc ggtcagccga ttcattttgt ccagtgttca ctggaagagg
     6241 cactgaaagc gcggcaagat cagcaacgtt atttcgaaca attagcgtgg cggctgcaca
     6301 atgaaaattg acctgaacgc cgatctgggc gaaggctgca ccagcgacgc agagctatta
     6361 acgctggttt cctctgccaa tattgcctgt ggatttcatg caggcgatgc gcaaaccatg
     6421 caggcttgcg tgcgtgaagc aataaaaaat ggtgtcgcga ttggcgctca tccgagtttt
     6481 cccgacagga aaaattttgg tcgcagcgct atgcagctgc cgccagaaac cgtttacgcc
     6541 cagacgctgt atcaaattgg cgcgctggca acgattaccc gtgcgcaagg tggtgtaatg
     6601 cgtcatgtca aaccgcacgg catgttatac aaccaggcgg cgaaagaggc acaactggca
     6661 gacgccatcg ccagagcggt atacgcttgc gatccagcat tggttctcgt tgggctggca
     6721 ggaagcgagc tgattcgtgc aggcaagcaa tatggcctga caacgcgcga ggaagtgttt
     6781 gccgatcgcg gttatcaggc tgacggctcg ctggtgccgc gaagccagtc aggcgcgctg
     6841 attgaagacg aagaacaggc gcttgcgcaa acgttggaga tggtgcaaca cggcagagtc
     6901 aaaagtatca ccggcgaatg ggcaacggtc acggcgcaaa cggtctgcct gcatggcgac
     6961 ggcgagcacg cactggcgtt cgcccgccga ctacgctctg catttgccga aaaggggatt
     7021 gttgtcgcag cataaccccg attaataaag aatgaaaaaa ggatatcacc atgcctgaag
     7081 gcccggagat ccgccgtgca gcggataacc tggaggcggc gatcaaaggc aaaccactaa
     7141 ctgatgtctg gtttgccttc ccgcagttaa aaacttatca atcacaactt atcggtcaac
     7201 acgttaccca tgtggaaacg cgtggtaagg cgttgttaac tcatttttcc aacgacttaa
     7261 cgctctacag ccataatcag ctttatggcg tctggcgcgt ggttgatacc ggcgaagagt
     7321 cgcagaccac gcgagtattg cgggtaaaac tgcaaacggc tgacaaaagc attctgcttt
     7381 atagcgcctc ggatattgag atgttgaccc cggaacaact gaccacgcat ccgtttttac
     7441 aacgcgttgg tcccgatgtg ctggatccga atctgacgcc ggaggtggtg aaagaacggt
     7501 tattgtcgcc gcgctttcgt aaccgtcagt ttgctggatt actgctcgat caggcgtttc
     7561 tggctgggct tggcaattat ttgcgtgtgg agatcctctg gcaggttggg ttgactggaa
     7621 atcataaagc gaaagacctc aatgcggcgc aactggatgc actcgcacac gcgttactgg
     7681 atattcctcg gttttcttac gctacgcggg ggcaggtgga tgagaataag catcatgggg
     7741 cgctgtttcg ctttaaggtt tttcatcgtg atggcgaact gtgcgaacgc tgtggcggca
     7801 tcattgagaa aaccacgctg tcatctcgcc cgttttactg gtgtccgggc tgccagcact
     7861 aggccgtacg tttcggcgca taggttgaaa taaaccgcgc aatggcaggc cctgtcagca
     7921 aaatactgaa caggcgcagg gtttgcatcg ccataatgag cgccatatcg gcattgctcc
     7981 ctgcggcgat gaccgccacg gtatcaagcc cgccggggct ggtggcgagg taggcagtca
     8041 taaaatcaat gtgcataaac cgggtcagtc cccacgccat acccgcacaa atagccagca
     8101 gagcaaaaat cgacagcagg atttgtggta gcgggcgtag tgcccgcagt aagatttgtt
     8161 tatcgaaacc aaggccaatc cgccagccaa ttgccatata cgccatcgcc agtagccatt
     8221 ccggtagttc gatggtgatg agctgaccag actggagcac tgcgcccgcc agcatcggga
     8281 tgagcatcgt gccggaagga agacgcagca ggcatcccac tgtaccggca acgaccgcga
     8341 gcaaaatagt aagcaggaga ttaatgctca ctggcgggaa ccagacgata tgctggttaa
     8401 ccgcttcagc gttatcgccc agcatcattc gcgtgaccag aactgcggcc cccgcgacaa
     8461 acagcactcg cagatactgc ataaacgcca ccaggcggat atctgcgccg taatcctgcg
     8521 ccattgcgac cattgccgcc gccccgccag gcgaggagcc ccaggcacca gtatttccgg
     8581 gcagtgagct atagcgcacc agtagccatc ccacgatagc gctggaaagc aacgtcacca
     8641 gtaaaatagc taacacaatc ggccagttaa cctccagcgt ggtcaggatc gaaccggtca
     8701 agttttgcgc aatcatgcag ccaagaatgg cctgcgcggc gagaaaagcg gagcggggga
     8761 gttgcagggt aaccccgcgc atactgaaga tgatcccggc gatcatcggt ccaagtaaga
     8821 gtgccgccgg gagatgtaat ccgaggaaac caatagaaag gagaagtgat agcacacata
     8881 acattcccca ctgcaaaact ggcatccttt gctccctcat tattttctct ttggtgtatc
     8941 gatacagcat aaagaatgcc gggcatggaa aaaataacgg taatcaataa aagccggaaa
     9001 attggcaatt aaccgaagaa gccctctccg gctggaaagg gcaataggct aaatcagttg
     9061 tagttaattt tgaaaagtac cactgtatca aagggaccaa cgtcgatggg cgtacccggc
     9121 gtggatgaca attctgccgt gtaggttttt tcatacacta acaggtcgct ggtaaaatcg
     9181 gcatactcac tgtatttgtt gaaagtatag ggtgaggcat tttcatccaa caatcgaagc
     9241 ttcaagccat tgccaatgag cagtgattta tcttcgtcga taagggtttc atcggtataa
     9301 aacgaagagc tgactttaaa accgtcggta cactggtcgt tttgtgattt ggttgtagtt
     9361 attttaaatt gctgcgacat cgtatggcga cgaatatcca gcacgttaaa tcggccaaaa
     9421 tcaacgacct gggtttcagg tgagatagca aagttgacgc tgcaatccag cacgcggata
     9481 ttttccagac cggtaatgtg atatttcaga ttcttcgcgg tagggtcttc gttcacgctg
     9541 cctgcgccat caaactgtac gacgatgtaa tcgctgagtt tgctctgata atcatggggc
     9601 ggcatggcgc gaactttaac gtaaagccgc atacgcgcca caaacgtccg cgatttgtgc
     9661 ataacggttg gatcgccgga gcaaagttta ttttgccagc ccatcgcaat gatctgctcc
     9721 ggggtgtaaa tatcaatatt cttactgtcg atgcactgat tcgtgtcgat gcggcttttc
     9781 ccaaggcttg cgtcatagtc cacgccttca taggttacgc ccaattgata atagggatcc
     9841 tgaataccgg ggtaagggtt tacccaggcg tagacgtgct cgctttcaaa attaccgttt
     9901 gtgttgttgt cacagtagac cggaattttt atatcgtcag attcccagat tttatcgccg
     9961 ggtttggaat tggccggaac ggcgaagggc ataatagctt ccgatttttc tacagcgccg
    10021 tttgcggtac caaaatagca gttcagcgcc agcgccgctt ttagcggcaa aaaaatcaga
    10081 cagatggcga gcaacacgcc tttggcgaat ctcatttttt attctccacc ggctgcaggc
    10141 ggcattgccg ctggcaggag agcgtgttca tacgcaatgc gccgtagtcg tccatgtagc
    10201 caagataaaa ctgcttgccg ctatagctgc ccgtgctggt ggtgaccgaa ctgaacggtg
    10261 cgaccatcgt gcttttaaag ccaggcagca cgcctttctc atcccgccca agataggcaa
    10321 tagtcagata gtatccggtc ggggttttca gggtaagctg gttattctgc tggctaacct
    10381 gtagctgttg ctcaacatga tcgcccattt ttttgcgtaa tgcggcgggt cgccagaata
    10441 gcttaatgcg actttggacg gcgacttgca ggatcgcatg gccctcgctg ccttccggga
    10501 caggcggaat ttcacgcagg ttatagtaaa agagagattc gcgatcggtg ggtaatttcg
    10561 cggtggcggc ttgtttcatg atccgaacct gcgaagtcgc ttttggctcc agtcgctgga
    10621 taggcggcag ggcgacgaga aattcatcgc ttttctctcc cttttcattt tctatccatg
    10681 aataggcgag gtaggggagt ttatcgctgc ggttttccac gcgcaggctg gttgctttat
    10741 ctttgctatt aaagatgatg cgcgtgcggt caggctgcac tgcgccgtgg ctgcacatac
    10801 tggcaaccag aagaatcaaa gggaatcctt taacaaatgt catcgtcttt tccttaatta
    10861 atgacagggt aaaatcaggc gcttagtcac atcttccaga tgttccggca gatgaatggc
    10921 acacgttttc tggtcgcccc agtgcacggt aaattgttga ttttcatcga ccccactcag
    10981 ccatgcgtga ccgttttcgc caaccatccc gacgctgaca ccgccttcag cctggcgaac
    11041 atcggcaccg agcggcggga aatgcccgtt agcatcgctg ataatgacgt tgagatcttt
    11101 cccggcgcgg gacgccagcg atttaaagcc gatggcgcct tcggtccagg tttctttcac
    11161 cacgttttcg gaaacggtaa cgccgtcagg caagtcgttc atattgaccg ccaccgtcgt
    11221 aggctggtag ctggaaacaa acggtaccac ggcaatacca aagcggttgg tgtagtcaat
    11281 attgccctga atcggaatat cgcccacgcc gtcggtgctg accatcaggc gtggttcatt
    11341 ggtggaactg cggcgatgga atgccgcgcc atgttgagtc gcggtaaacg agccgctcca
    11401 actggcactg gcggaagtgt aatcattggc ggcataggtt ccggtcagat cccattcccc
    11461 cgccgaactg aggtgttggt aattgccgct gacctgcgca ccgttatccg ggcgatcgga
    11521 ttgtattcca gccgacattc cccaactgtt tcgttcatcc agggtatcgt tccacgacat
    11581 gcggtgggtg gtagtattac tgttgttctg catgtcataa cccagtcgac cactttcccc
    11641 aatcggtaag gagatcgaaa agtaaatctg gttgtcgcga tctttatctt cataatgggt
    11701 ggtgttgaac gaggtcgaga cagaaatatc tttccagtcg ccaatgtcga cgttaaaacc
    11761 gacggtaatg ttggccgtgg tggaggtatc cgcattccac cagctctgat gcaggatgtt
    11821 ggcgtagaga ttaagattca gcagagtaat aggctgaccg aaggagaggc tgattgtttg
    11881 cttttcatcc tgcgcgtcgg cgtcattgta tttgtgatca atgtagttag cgtagctgtg
    11941 aaaatggcga tcggagaagc gataggctgc caatgagatg gtgctgttgg tcgcatcaac
    12001 ctgtttgctg tagttaaagc ggtagctgta accctgctca tcctgttggg tatcaaaatg
    12061 gctgtcggcc caggtcacgt caaacgacag cgcccccatc cagagcatat tttgcccaat
    12121 acccagcgcg ccggaacgat agtcatcgcc tgcgagaagc atgccgccat atagtgaagt
    12181 gttggagagc attccccagg agacttcatg gctaatgaag gtttcatcct cggtgtggtg
    12241 cgacatagag gaacgcgggc gaccggcagc cagtttatag cgcacctggc cctgacgcgt
    12301 caggaacggg gtagaggccg ctgaaacctg gaagttattc acccgtccat cttcttcgct
    12361 cactttgaca tccagtgtac cttgcactga ctgattcagg tcatcaataa taaatgggcc
    12421 tggtggaact tttttctggt aaatcacgcg accagaatgg cttatcgtga ccgtggcgtt
    12481 agtctgtgcg atgccgctga tttgcggcgc gtagccgcgt aactcccagg ggagcattcg
    12541 atcgtcgctg gctaacgccg caccggtata ggagaaacca tcaaaaatgt tagagctaaa
    12601 atcggtttcg cccagggtta atcgggaacc tatttgcggt aacgggcgga acaggtaggt
    12661 ccgtgaaatc gccccagatt gttcacggtt gtcgccgcta tcggactggc taagctgata
    12721 atcgctgcgc agacgccatg ccccggcgtt gaggcccgtg gtgccatagg cgttcaggtt
    12781 gttgctgctg ctgccgcttt gtgggcgata agtgctggca aacaggttgt aatccatcag
    12841 gaagccagga ataccgttat tccacgttga gggtggcgtc cagttttctg aatgccacgc
    12901 cagccatgcc tgcgggattg taatattgag ctgttgattt gcctgatcga aagtaaagat
    12961 gatttcaggg aagacactaa aatcgacaca ttcttttatt gccggtaatt ttttacgaaa
    13021 gtcagattta agtccgaaga gttcgatcag cgattcattg atacaaggaa taattttatc
    13081 gccagatttt tgccagcgaa tttgttgccc attactgatt ttattattat taaccgtaac
    13141 actaacaaag taatcgccgg gagctacgac gcctttttga ttgagcaacg aaatatcaac
    13201 gctatcccga atcgacttat cgagaacgtt aaggttaaat tcagctgtca gcgcaggagg
    13261 ggttaccatt gccagacagg aaagaacaga gagtcgataa atattcacgg catccatacc
    13321 tgaacaatat tcgcaaaagg cggctaatca gccgccttaa aaaggattat tggtaatcca
    13381 gaacgtaagt tgcgttagca gtaacggccc ccgcggtaac cgaaactgcg ttatccatct
    13441 gttccatcca ggcaaagaag tttaacgttt gttctgttga acttgccaat aagtcaattg
    13501 ttggcgcttc tgttcccaat gtaacaaatg attcatcttt gttcatgaga cgtacgccaa
    13561 cgccggtagc ctcgccagca ctgatgttac taaggagcgg ggttgcacct gtcgttttag
    13621 ccgtgctgtc gaaggtcacg cctacctttg agaccacgcc acccttgcca tcatcccagc
    13681 ctgcaatatc acagttgatt aagcgaatat tgacaggttt tttctcgcta tgatgttctc
    13741 gatttatgtg ggatgtggtt acttcaccca ggtcaatgtt catatcaagg tcatcagggt
    13801 gaatttcaca tggtgcttca ataacttcac ctttaaagtg gatttcacca gatccttcat
    13861 ctgcagcaaa aacaggtgaa gcgaatagta gagatatggc cagggcggcc agtgtttttt
    13921 gtcctttaaa catacatgaa tcctt
//
LOCUS       DAZKIP010000112        13836 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZKIP010000112 DAZKIP010000000
VERSION     DAZKIP010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.113"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            190..1299
                     /gene="livK"
                     /locus_tag="ACHOUK_003647"
     CDS             190..1299
                     /gene="livK"
                     /locus_tag="ACHOUK_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFP9899017.1"
                     /translation="MKRNAKTIIAGMIALAISHTALADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1347..2273
                     /gene="livH"
                     /locus_tag="ACHOUK_003648"
     CDS             1347..2273
                     /gene="livH"
                     /locus_tag="ACHOUK_003648"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFP9899018.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2270..3547
                     /gene="livM"
                     /locus_tag="ACHOUK_003649"
     CDS             2270..3547
                     /gene="livM"
                     /locus_tag="ACHOUK_003649"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFP9899019.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3544..4311
                     /gene="livG"
                     /locus_tag="ACHOUK_003650"
     CDS             3544..4311
                     /gene="livG"
                     /locus_tag="ACHOUK_003650"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFP9899020.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4313..5026
                     /gene="livF"
                     /locus_tag="ACHOUK_003651"
     CDS             4313..5026
                     /gene="livF"
                     /locus_tag="ACHOUK_003651"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFP9899021.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5145..5891
                     /locus_tag="ACHOUK_003652"
     CDS             5145..5891
                     /locus_tag="ACHOUK_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFP9899022.1"
                     /translation="MHYAVVVMLELLCCIHAYRTGQERYWIFIIFCFPVIGCVAYFVI
                     VMLPETGADRHGHTLLMRLQDKLNPERHLRKLTEELAIAETNQNHYALANELARLGRY
                     HEAVPHYQQALSGIFAHEAAMMLSLAQAQFAIQEFAACQQTLEDVMRYNPDFQSADGH
                     LLFARTLAAQEKYADAESEFEVLISYYPGPQARIYYAEMLAKMSRLREANEQYVAVVD
                     TAKRSRPHYRKHHREWIKTANERLKQSVVQ"
     gene            6251..7567
                     /gene="ugpB"
                     /locus_tag="ACHOUK_003653"
     CDS             6251..7567
                     /gene="ugpB"
                     /locus_tag="ACHOUK_003653"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFP9899023.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            7665..8552
                     /gene="ugpA"
                     /locus_tag="ACHOUK_003654"
     CDS             7665..8552
                     /gene="ugpA"
                     /locus_tag="ACHOUK_003654"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFP9899024.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSRFVGLDNFVALFHDSYYIDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVMQFRYVESKVRYQ"
     gene            8549..9394
                     /gene="ugpE"
                     /locus_tag="ACHOUK_003655"
     CDS             8549..9394
                     /gene="ugpE"
                     /locus_tag="ACHOUK_003655"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFP9899025.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            9396..10466
                     /gene="ugpC"
                     /locus_tag="ACHOUK_003656"
     CDS             9396..10466
                     /gene="ugpC"
                     /locus_tag="ACHOUK_003656"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFP9899026.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDICINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIALPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLPENQLHLFDGETGQRV"
     gene            10463..11206
                     /gene="ugpQ"
                     /locus_tag="ACHOUK_003657"
     CDS             10463..11206
                     /gene="ugpQ"
                     /locus_tag="ACHOUK_003657"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFP9899027.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYGKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARELWAGMTPPLLSSFEIDALEAAQQAV
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(11193..11633)
                     /locus_tag="ACHOUK_003658"
     CDS             complement(11193..11633)
                     /locus_tag="ACHOUK_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFP9899028.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPGTPQPDIPLKTIGP"
     gene            11753..13498
                     /gene="ggt"
                     /locus_tag="ACHOUK_003659"
     CDS             11753..13498
                     /gene="ggt"
                     /locus_tag="ACHOUK_003659"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFP9899029.1"
                     /translation="MIKPTFLRRVAIAALFTGSCFSTVAAPPAPSPVSYGVEEDVFHP
                     VRAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLI
                     RSKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALD
                     KYGTMPLNKVVQPAFKLARDGFIVNDELADDLKTYGSEVLPNHENSKAIFWKEGEPLK
                     KGDKLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVER
                     TPISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYA
                     YADRSEYLGDPDFVKVPWQALTNKAYAKSIAEQIDINKAKPSSEIRPGKLAPYESNQT
                     THYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGG
                     DANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSLIITTVLQMVVNSIDYGMNVAE
                     ATNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELY
                     GASDPRSVDDLTAGY"
BASE COUNT         3092 a         3485 c         3935 g         3324 t
ORIGIN      
        1 caataaattc agaatattat ctgttcttaa tcgactgaaa aatagggact ttaatcgcta
       61 ttatcacaaa atactgcgct aaacccttaa tcagacaggc aaaaacagtg cagtataaaa
      121 aaagaacagt ctgatttgtt aacacataaa aacaaagcaa cacaacatca cgaatgggga
      181 tttttgacta tgaaacggaa tgcgaaaact atcatcgcag ggatgattgc actggcaatt
      241 tcacacaccg ctttagccga cgatattaaa gttgccgttg tcggcgcgat gtccggcccg
      301 attgcccagt ggggcgatat ggaatttaac ggcgcgcgtc aggcgattaa agacattaat
      361 gccaaagggg gaattaaagg cgataagctg gttggcgtag aatatgacga cgcctgcgac
      421 ccgaaacaag ccgttgcggt cgccaacaaa atcgttaatg acggcattaa atacgttatt
      481 ggtcatctgt gttcttcttc tacccagcct gcgtcagata tctatgaaga cgaaggtatt
      541 cttatgatct cgccgggagc gaccaacccg gagctgaccc aacgcggtta tcaacacatt
      601 atgcgtactg ccgggctgga ctcttcccag gggccaacgg cggcaaaata cattcttgag
      661 acggtgaagc cccagcgcat cgccatcatt cacgacaaac aacagtatgg cgaagggctg
      721 gcgcgttcgg tgcaggacgg gctgaaagcg gctaacgcca acgtcgtctt cttcgacggt
      781 attaccgccg gggagaaaga tttctccgcg ctgatcgccc gcctgaaaaa agaaaacatc
      841 gacttcgttt actacggcgg ttactacccg gaaatggggc agatgctgcg ccaggcccgt
      901 tccgttggcc tgaaaaccca gtttatgggg ccggaaggtg tgggtaatgc gtcgttgtcg
      961 aacattgccg gtgatgccgc cgaaggcatg ttggtcacta tgccaaaacg ctatgaccag
     1021 gatccggcaa accagggcat cgttgatgcg ctgaaagcag acaagaaaga tccgtccggg
     1081 ccttatgtct ggatcaccta cgcggcggtg caatctctgg caactgccct tgaacgtacc
     1141 ggcagcgatg agccgctggc gctggtgaaa gatttaaaag ctaacggtgc aaacaccgtg
     1201 attgggccgc tgaactggga tgaaaaaggc gatcttaagg gatttgattt tggtgtcttc
     1261 cagtggcacg ccgacggttc atccacggca gccaagtgaa aatcccaccg cccgtgaaat
     1321 gcgggcgggt ttagaaaggt taccttatgt ctgagcagtt tttgtatttc ttgcagcaga
     1381 tgtttaacgg cgtcacgctg ggcagtactt acgcgctgat tgccatcggc tacaccatgg
     1441 tttacggcat tatcggcatg atcaacttcg cccacggcga ggtttacatg atcggcagct
     1501 acgtctcatt tatgatcatc gccgcactga tgatgatggg cattgatacc ggctggctgc
     1561 tggtagcggc gggattcgtc ggcgcaatcg tcattgccag cgcctacggc tggagtatcg
     1621 aacgggtggc ttaccgcccg gtgcgtaact ctaagcgcct gattgcactc atctcggcaa
     1681 tcggtatgtc catcttcctg caaaactacg tcagcctgac tgaaggttcg cgcgacgtgg
     1741 cgctgcccag cttgtttaat ggtcagtggg tagtgggtca tagcgaaaac ttctctgcct
     1801 ctattaccac catgcaggcg gtgatctgga ttgtcacctt cctcgccatg ctggcgctga
     1861 cgattttcat tcgttattcc cgtatggggc gcgcctgccg tgcctgcgcc gaagatctga
     1921 aaatggcgag cctgcttggc attaacaccg accgggtgat tgctttgacc tttgtgattg
     1981 gcgcggcgat ggcggcggtg gcgggcgtac tgctcggtca gttctacggc gtcattaacc
     2041 cctatatcgg ctttatggcc gggatgaaag cctttaccgc tgcggtgctc ggtgggattg
     2101 gcagcattcc gggagcgatg attggcgggc tgattctggg gattgcggag gcgctctctt
     2161 ctgcctacct gagtacggaa tataaagatg tggtctcatt cgccctgctg attctggtgc
     2221 tgctggtgat gccgaccggt attctgggtc gcccggaggt agagaaagta tgaaaccgat
     2281 gcatattgca atggcgttgc tctctgccgc gatgtttttt gtcctggcgg gcgtctttat
     2341 gggcgtacaa ctggagctgg atggcaccaa actggtggtc gacacggctt cagacatccg
     2401 ttggcagtgg gtgtttatcg gcacggcggt ggtctttttc ttccagcttt tgcgaccggc
     2461 tttccagaaa gggttgaaaa gcgtttccgg accgaagttt attctgcccg ccattgatgg
     2521 ctccacggtg aagcaaaagc tgtttctcgt ggcgctgctg gtgcttgcgg tggcgtggcc
     2581 gtttatggtc tcacgcggga cggtggatat cgccaccctg accatgatct acattatcct
     2641 cggactcggg ctgaacgtgg tggtagggct ttctggtctg ctggtgctgg ggtacggcgg
     2701 cttttacgcc atcggcgctt atacctttgc gctgctcaat cactattacg gcttgggctt
     2761 ctggacctgt ctgccgatag ccgggttgat ggcggcggcg gcgggcttcc tgctcggctt
     2821 cccggtgctt cgcttgcgcg gtgactatct ggcgatcgtt accctcggtt tcggcgaaat
     2881 tgtgcgcata ttgctactca ataacaccga aattaccggc ggtccgaacg gtatcagtca
     2941 gatcccgaaa ccgacttttt tcggccttga gttcagccgt accgctcgtg aaggcggctg
     3001 ggacacgttc agtaatttct ttggcctgaa atacgatccc tccgatcgtg tcattttcct
     3061 ctacctggtg gcgttgctgc tggtggtgct aagcctgttt gtcattaacc gcctgctgcg
     3121 gatgccgctg ggtcgcgcct gggaagcgtt gcgtgaagat gaaatcgcct gccgttcgct
     3181 gggcttaagc ccgcgtcgta tcaagctgac tgcctttacc atcagcgccg cgtttgccgg
     3241 ttttgccgga acgctgtttg ctgcgcgtca gggctttgtc agcccggaat ccttcacctt
     3301 tgccgaatcg gcgtttgtgc tggcgatagt ggtgctcggc ggtatgggct cgcaatttgc
     3361 ggtaattctg gcggcaattt tgctggtggt gtcgcgcgag ttgatgcgtg atttcaacga
     3421 atacagcatg ttaatgctcg gtggtttgat ggtgctgatg atgatctggc gtccgcaggg
     3481 cttgctgccc atgacgcgcc cgcaactgaa gctgaaaaac ggcgcagcga aaggagagca
     3541 ggcatgagtc agccattatt atctgttaac ggcctgatga tgcgcttcgg cggcctgctg
     3601 gctgtgaata acgtcaatct tgaattgtac ccgcaggaga tcgtctcgtt aatcggtcct
     3661 aacggtgccg gaaaaaccac ggtctttaac tgcctgaccg gattctacaa acccaccggc
     3721 ggcactattt tactgcgcga tcagcatctg gagggtttgc cggggcagca aatcgcccgc
     3781 atgggcgtgg tgcgtacctt ccagcatgtg cgtctgttcc gcgaaatgac ggtaattgaa
     3841 aacctgctgg tggcgcagca tcagcaactg aaaaccgggc tgttctctgg cctgttgaaa
     3901 acgccttctt tccgtcgcgc ccaaagtgaa gcgctcgacc gcgccgccac ctggcttgag
     3961 cgcattggtt tgctggaaca cgccaaccgt caggcgagta acctggccta cggtgaccag
     4021 cgccgtctgg agattgcccg ctgcatggtg acgcagccgg agattttaat gctcgacgaa
     4081 cctgcggcag gtcttaaccc gaaagaaacg aaagagctgg atgagctgat tgccgaactg
     4141 cgcaatcatc acaacaccac tatcttgttg attgaacacg atatgaagct ggtgatggga
     4201 atttcggacc gaatttacgt ggtcaatcag gggacgccgc tggcaaacgg tacgccggag
     4261 caaatccgta ataacccgga cgtgatccgt gcctatttag gtgaggcata agatggaaaa
     4321 agtcatgttg tcctttgaca aagtcagcgc ccactacggc aaaatccagg cgctgcatga
     4381 ggtgagcctg catatcaatc agggcgagat tgtcacgctg attggcgcga acggggcggg
     4441 gaaaaccacc ttgctcggca cgttatgcgg cgatccgcgc gccaccagcg ggcgaattgt
     4501 gtttgatgat aaagacatta ccgactggca gacggcgaaa atcatgcgcg aagcggtagc
     4561 gattgtcccg gaagggcgtc gcgtcttctc gcggatgacg gtggaagaga acctggcgat
     4621 gggcggcttt tttgccgaac gtgatcagtt tcaggagcgc ataaagtggg tgtatgagct
     4681 gtttccacgt cttcatgagc gtcgtgttca gcgggcaggc accatgtccg gcggtgaaca
     4741 gcagatgctg gcgattggtc gcgcgctgat gagcaatccg cgtttgttgt tgcttgatga
     4801 gccatcgctc ggtcttgcgc cgattatcat ccagcaaatt ttcgacacca tcgagcagct
     4861 gcgcgagcag gggatgacta tcttcctcgt cgagcagaac gccaaccagg cgctgaagct
     4921 cgctgatcgc ggctacgtgc tggaaaacgg ccatgttgtg ctctccgata ctggtgatgc
     4981 gctgctggcg aacgaagcag tgagaagtgc gtatttaggc gggtaaatta tcgataaccc
     5041 ggcagctaag ttctgccggg ttattcgtcc gtatacagac ttgtttcaac tgaccgggag
     5101 gattaaaacc agaccactta tttgccacgg aaaggttccc tcccatgcat tatgccgttg
     5161 ttgtcatgct tgaactgctt tgctgtattc acgcttaccg taccggacaa gagcggtact
     5221 ggatttttat cattttctgt tttccggtga ttggctgcgt ggcgtatttc gtcatagtga
     5281 tgctgcctga aacgggcgct gaccgtcacg ggcatacttt gctgatgcgc ctgcaggaca
     5341 aactcaaccc tgagcgccac ctgcgcaaat taaccgaaga actggcgatt gccgagacga
     5401 atcaaaacca ttatgcgttg gctaatgaac tggcgcgtct ggggcgttat cacgaagctg
     5461 ttccccatta tcagcaggcg ttaagcggga tattcgccca tgaagcggca atgatgctaa
     5521 gcctggcgca ggcacagttt gctattcagg agttcgccgc ctgtcagcag acgctggaag
     5581 atgtgatgcg ttataaccct gattttcagt cggcagacgg gcatttgtta tttgccagaa
     5641 cgttagccgc acaagaaaaa tacgctgatg cggaaagcga gtttgaagta ttgatttctt
     5701 attaccccgg cccgcaggcg cgtatttact atgcggaaat gttggcgaaa atgagtcggt
     5761 tacgtgaagc caacgagcag tatgttgccg tggttgatac ggctaaacgc tcaagacctc
     5821 attatagaaa acaccaccgg gaatggatta agaccgccaa tgagcggctg aagcagagtg
     5881 tggtgcaatg aaaatcttct ttgccgttag accagtgtcg cggcaatctt ttgttaaaat
     5941 tgaaaaatgt agctctcatc gggcagcaaa attggacgca gaatttgctg ccatttttgc
     6001 tcttcatggt aaggagttct ggcgattagc tggccgttaa tcgcctcttt tcagtgcatt
     6061 ctcgggatat ccgtgcccca tctcccattt tccctcaccc gtaccgtcac cgccttgtca
     6121 tctttctgac accttactat cttacaaatg taacaaaaaa gttatttttc tgtaattcga
     6181 gcatgtcatg ttaccccgcg agcataaaac gcgtgaattc gcgcattcgg tacaacaaga
     6241 gagataaacg atgaaaccgt tacattatac agcttcagca ctggcgctcg gactggcgtt
     6301 aatggggaat gcacaggcag tgacgaccat tccgttctgg cattctatgg aaggggaact
     6361 gggtaaagag gtggattctc tggcccaacg ttttaacgcc gaaaacccgg attacaaaat
     6421 tgtaccgacc tataaaggca attacgaaca gaatttaagc gcggggattg ccgcatttcg
     6481 taccggcaat gctccggcta ttttgcaggt ttatgaagtt ggcaccgcca ccatgatggc
     6541 gtcgaaagcc attaaaccgg tttatgacgt gtttaaagag gcggggattc aattcgatga
     6601 gtcgcagttt gtgccgacgg tttccggtta ctactccgac agcaaaacgg gccacttact
     6661 ctcccagcca ttcaacagct cgactcccgt tctctattac aacaaagacg ccttcaagaa
     6721 agcggggtta gacccggaac aaccgccgaa aacctggcag gatctggcgg actatgccgc
     6781 aaaactaaaa gcctccggca tgaagtgcgg ctacgccagc ggctggcagg gctggatcca
     6841 actggaaaac tttagcgcct ggaacggtct gccgtttgcc agcaaaaaca acggctttga
     6901 cggcacggat gcggtgctgg agttcaacaa gccggagcag gtgaaacaca tcgccatgct
     6961 cgaagagatg aacaagaagg gcgatttcag ctacgtcggg cgtaaggatg aatccaccga
     7021 gaagttctat aacggtgatt gcgctatgac caccgcctct tccggttctc tcgctaatat
     7081 tcgcgagtac gccaaattca actatggcgt aggcatgatg ccttacgacg ccgatgcgaa
     7141 agatgcaccg caaaacgcca ttatcggcgg ggccagcctg tgggtgatgc agggcaaaga
     7201 taaagaaacg tataccggcg tggcgaagtt cctcgacttc ctcgcgaagc cagaaaacgc
     7261 tgccgagtgg catcagaaaa ccggttatct gccaatcacc aaagcagcgt atgacctgac
     7321 ccgtgagcag ggcttttatg agaaaaaccc aggggcggat accgcgacgc gtcagatgct
     7381 gaataagccg ccgttgccgt tcaccaaagg gctgcgtctg ggcaacatgc cgcagatccg
     7441 cgtgattgtg gatgaagagc tggagagcgt gtggaccggt aagaagacac cacagcaggc
     7501 actggatacc gccgttgagc gtggaaatca gttgctgcgc cgctttgaga aatcgacgaa
     7561 gtcttaatca gtgtaatgtc ggatgcgttt cgcttatctg acctggcatc gcgtgtaggc
     7621 cggataagcg aagcgcatcc ggcacagttc aggaattaac cgtaatgtca tcatcccgtc
     7681 cggtgttccg ctcgcgctgg ctgccttatc tgctggtcgc gccgcagctc atcatcaccg
     7741 ttatcttttt tatctggcct gcgggcgaag cgttgtggta ctcgctgcaa agcgtcgatc
     7801 cgtttggttt ctccagccgg tttgtcggcc tggataactt cgtcgcgctg tttcatgaca
     7861 gctactatat cgactccttc tggacgacga taaaattcag cacctttgtc accgtcagcg
     7921 gtttgctggt atcgctgttc tttgcggcgc tggtggagta catcgtgcgc ggcagtcgtt
     7981 tctatcaaac cttgatgctg ctgccgtatg ccgtggctcc cgccgttgcc gccgtattgt
     8041 ggatcttcct gtttaacccc ggtcgcgggc tgatcaccca ttttctcgct gagttcggct
     8101 acgactggaa ccacgcgcaa aacagcggtc aggcaatgtt tctggtggtg tttgcctcag
     8161 tatggaagca aatcagctac aacttcctgt tcttctatgc cgcgctgcaa tccattcccc
     8221 gttcgttgat cgaagccgca gccatcgacg gtgcagggcc gattcgccgc ttctttaaga
     8281 ttgcgctgcc gcttatcgcc ccggtgagtt tcttcctgct ggtagtgaac ctggtgtatg
     8341 ccttcttcga caccttcccg gtgatcgacg ccgccacgtc cggcgggccg gttcaggcca
     8401 ccactacgct gatttataag atctaccgcg aaggctttac cggactggat ctggcttcgt
     8461 ctgccgcaca gtcggtggtg ttgatgttcc tcgtcatcgt gctgacggtg atgcagttcc
     8521 gctatgttga aagcaaggtg cgttaccaat gattgagaac cgtccgtggc tgacgatatt
     8581 cagccatacc atgctgatcc tcgggatcgc ggtgatccta ttcccgctgt acgtggcgtt
     8641 tgtcgcggcg acgctggata aacaggccgt ctacgccgcg ccgatgacgc tcatccccgg
     8701 cacgcatctg ctggaaaaca tccacaacat ctgggtaaac ggggtaggca caaatagtgc
     8761 gccgttctgg cggatgttgc ttaacagctt cgtgatggcg ttcagcatta cgctcggcaa
     8821 aattaccgtc tcgatgctct cggcattcgc cattgtctgg tttcgttttc cgctgcgtaa
     8881 cctctttttc tggatgattt ttatcaccct gatgttgccg gttgaagtac gtatcttccc
     8941 gacggtggaa gtcatcgcca acctgaagat gctcgacagc tacgcaggtt taacgctgcc
     9001 gctgatggcc tcggcgaccg cgacgtttct tttccgccag ttctttatga ccctaccgga
     9061 tgaactggtg gaagccgcgc ggatcgacgg cgcgtcgcca atgcgcttct tttgcgacat
     9121 cgtttttccg ttgtccaaaa ctaatctggc ggcgttgttt gtgatcacct ttatttacgg
     9181 ctggaaccag tatttgtggc cgttgttgat tattaccgac gtggatctcg gcaccaccgt
     9241 ggcagggatc aaagggatga tcgccactgg cgaaggcacc acagaatgga actcagtgat
     9301 ggcggcgatg ttgttaaccc tgatcccacc agtagtgatt gttttagtga tgcagcgtgc
     9361 cttcgtgcgc ggcctggtcg atagtgagaa ataagatggc aggactgaaa ttacaggcag
     9421 taaccaaaag ctgggatggc aaaacccagg tgattaaacc gctgaccctt gatgtggcgg
     9481 atggcgaatt tatcgtgatg gtcgggccgt caggctgcgg gaaatcgacg ctgctgcgca
     9541 tggttgccgg gctggagcgg gtgacggaag gcgatatctg tatcaacgac cagcgggtga
     9601 ccgaaatgga gccgaaagat cgcgggattg cgatggtgtt ccagaactac gcgctttatc
     9661 cgcatatgag tgttgaagaa aacatggcgt gggggctgaa aattcgcggc atgggcaagc
     9721 agcaaattgc cgagcgcgtt aaagaggcgg cgcgcattct ggaactggac ggtctgctta
     9781 agcgccgccc gcgcgagctt tccggcggtc agcgtcagcg tgtggcgatg ggccgggcga
     9841 ttgtgcgcga tccggcggtg ttcctgtttg atgagccact ctctaacctc gatgccaagc
     9901 tgcgcgtaca gatgcgtctt gaactgcaac agctgcaccg tcgcctgaaa acgacttcac
     9961 tctacgttac tcacgatcag gttgaggcga tgaccctcgc ccagcgagta atggtgatga
    10021 acggcggcgt tgccgaacag attggcacac cagttgaagt ctacgaaaag cccgccagcc
    10081 tgtttgtggc gagttttatt ggcagcccgg cgatgaatct gctgacaggc cgcgtgaata
    10141 acgaaggcac gcacttcgag ctggacggcg gcattgcgct gccgctaaac ggtggctacc
    10201 gtcagtatgc agggcgtaaa atgactctcg gcattcgccc ggaacatatc gcgctaagct
    10261 cgcaggcaga aggcggcgtg ccgctggtga tggacacgct ggagatcctc ggcgcagata
    10321 acctggcgca cggacgctgg ggcgaacaga agctggtggt acggctggcg catcaggagc
    10381 gcccgacggc aggcagcacg ctgtggctgc atctgccgga aaatcagcta catctttttg
    10441 atggtgaaac aggacaacga gtatgagtaa ctggccttat ccccgcatcg tcgcccaccg
    10501 tggcggcggt aaactggccc cggaaaacac cctggcggca atcgacgtcg gggcaaaata
    10561 cggtcataag atgatcgaat ttgacgcgaa gttgtcgaaa gatggcgaaa tcttcctgct
    10621 ccatgacgac aatctcgaac gtaccagcaa cggctggggc gtcgcgggtg aactgaactg
    10681 gcaggattta ctacgtgtgg atgcaggcag ttggtacggc aaagcgttta aaggtgagcc
    10741 gctgccgttg ctttcgcagg tggcggaacg ctgtcgcgaa catgggatga tggcgaatat
    10801 cgaaatcaaa cccaccactg gcactgggcc attaacgggc aaaatggtgg cgctggcggc
    10861 acgcgagctg tgggccggta tgacgccgcc gctactgtca tcgtttgaga ttgatgcttt
    10921 agaagccgca caacaggcag taccagaact gccgcgcggt ttgttgctgg atgagtggcg
    10981 cgacgactgg cgagaactga ccgcgcggct gggctgcgtc tctattcatc tcaatcataa
    11041 gttgctcgat aaagcgcgag tgatgcagtt gaaagacgcc ggattacgga ttctggttta
    11101 taccgtcaat aaaccccagc gcgcggcaga gttgctgcgc tggggcgtgg attgcatctg
    11161 taccgatgcg attgatgtga ttggtccgaa ctttacggcc caatagtttt caacggtata
    11221 tcaggctgcg gcgtgccggg ctgattcagc atgtcgccgt ttctcctctc cggcaacata
    11281 tgctgctgat tcaacgaact atccggatta cggttgctgt ttaacatccc gccattggtg
    11341 ttgggcagca tttgctgccg ggcgggattt cgctcccccg gctgtgactg caacacccgc
    11401 tgagaattat tgtttatctg gttttctaaa tgctgttgtt gcaactgagt ttgcgttttc
    11461 agttgctgat tcagcatccc tttttgctgg atttgctgcg tttgcatctg ggtttgcatc
    11521 cgctgctgac tggggatctg ataccccggc tggttagggt tgttcagagt attaatgggc
    11581 tgtgcaaagc cgacaaacgg caggagtgcc gtaagaatca gaagtcgttt catcgcgtat
    11641 cctcctctga agatattctt taagtttact cctttcctgc aatcatcagg attaattcag
    11701 agttatatac caggcttagc tggggttgcc ccttaatctc tggagaataa cgatgataaa
    11761 accgacgttt ttacgccggg tggccattgc tgctctgttc acaggaagtt gttttagtac
    11821 ggtagccgcg ccacctgcgc cgtctcctgt ttcttacggc gtggaggaag atgtctttca
    11881 tccggtacgc gcgaaacagg ggatggtggc atctgtagat gctactgcca ctcaggtggg
    11941 ggtggatatc ctcaaggagg gcgggaatgc cgttgatgcc gccgtggctg tgggctacgc
    12001 gctggcggta acgcatccgc aggcagggaa tctgggcggt ggtggtttta tgctgatccg
    12061 ctcgaaaaat ggcaatacca ccgctatcga tttccgcgaa atggcacccg ccaaagcgac
    12121 ccgcgatatg ttccttgatg accagggcaa cccggacagc aaaaaatcac tcacttcgca
    12181 tctggcttcc ggcacgcccg gtacggtcgc tggtttctcg ctggcgttgg ataaatacgg
    12241 cacaatgccg ttgaacaaag tggtgcagcc agcgtttaaa ctggcacgcg atggttttat
    12301 cgttaacgac gagctggctg acgatctcaa aacctacggg agcgaagtgc tgccgaatca
    12361 cgaaaacagc aaggctatct tctggaaaga gggcgagccg ctgaaaaagg gcgacaagct
    12421 ggtgcaggcg aacctggcaa agagcctgga gatgattgcc gaaaacggcc cggacgaatt
    12481 ctacaaaggc acgatagcgg aacagattgc ccaggagatg cagaaaaacg gtggcctgat
    12541 cactaaagaa gatttagcgg cgtataaagc ggtcgaacgc acaccgataa gcggcgatta
    12601 tcgcgggtat caggtttact ccatgccacc gccatcctcc ggcgggatcc atatcgtaca
    12661 aatcctcaat attctggaaa acttcgatat gaagaaatac ggttttggca gcgccgacgc
    12721 gatgcagatc atggcagaag cggagaaata tgcctacgct gaccgctcgg aatatcttgg
    12781 cgacccggat tttgtgaaag tcccgtggca ggcgctgacc aataaagcct atgccaaatc
    12841 tattgccgag caaatcgata tcaataaagc gaaaccgtcc agcgagattc gccccggcaa
    12901 gcttgcgcct tatgagagta atcaaactac ccattattcg gtggtggata aagacggtaa
    12961 cgcggtggcg gtgacctata cgctgaacac caccttcggt acgggtattg tcgcgggcga
    13021 gagcggtatt ttgctcaata accaaatgga tgatttctct gccaaaccgg gcgtaccgaa
    13081 cgtttacggg ctggtgggcg gtgatgccaa cgccgtcggg ccgaacaaac gcccgctgtc
    13141 atcgatgtca ccaaccattg tggtgaaaga cggtaaaacc tggctggtca ccggtagccc
    13201 tggcgggagc ctgatcatca ctacagtgct tcaaatggtg gtgaatagca tcgattatgg
    13261 catgaacgtc gccgaagcga ccaatgcgcc gcgtttccac catcagtggt tgccggacga
    13321 gctgcgtgtc gaaaaagggt ttagcccgga tacgctcaag ctgctggaag caaaaggtca
    13381 gaaagtggcg ctgaaagagg cgatgggcag tacacaaagc attatggttg ggccggacgg
    13441 tgagttgtac ggcgcatccg acccgcgctc ggtggatgat ttaacggcgg ggtactaagg
    13501 ttagcggccc tcttcgtggg aagagggctg ttttcttgaa caagataaat tagtgtctgg
    13561 attgctttta ctattgctcc gtaatatata atcataatta tcctgaatgc ttttaagggg
    13621 cttaggttat gctttctttt caagagtatg cagaatagtc tttcatttac cgtgaaaata
    13681 ggacgtgcga taattacttc ctgatattca ggaaaggaat taactatgct attaaataaa
    13741 ctgtagttat agttcatttt agagtgtaat ttattgattt aaatatcacg ctttgtgttg
    13801 tttatgtgca taatggcatt catcatgact ttgatc
//
LOCUS       DAZKIP010000113        13527 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZKIP010000113 DAZKIP010000000
VERSION     DAZKIP010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.114"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..268)
                     /locus_tag="ACHOUK_003660"
     CDS             complement(<1..268)
                     /locus_tag="ACHOUK_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899030.1"
                     /translation="MNISYVNSNKTTSLPIELDVQNNKDFSYAKDFFLYIETQLKIAK
                     DFCRPEEEVSSSIASTVFHAFIDLVNKIMGKKDCMYICTLCCFAE"
     gene            669..1382
                     /gene="aphA"
                     /locus_tag="ACHOUK_003661"
     CDS             669..1382
                     /gene="aphA"
                     /locus_tag="ACHOUK_003661"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFP9899031.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKNFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     SMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            1493..1909
                     /locus_tag="ACHOUK_003662"
     CDS             1493..1909
                     /locus_tag="ACHOUK_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFP9899032.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            1913..2269
                     /locus_tag="ACHOUK_003663"
     CDS             1913..2269
                     /locus_tag="ACHOUK_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFP9899033.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(2304..5126)
                     /gene="uvrA"
                     /locus_tag="ACHOUK_003664"
     CDS             complement(2304..5126)
                     /gene="uvrA"
                     /locus_tag="ACHOUK_003664"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFP9899034.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLYARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            5381..5917
                     /gene="ssb1"
                     /locus_tag="ACHOUK_003665"
     CDS             5381..5917
                     /gene="ssb1"
                     /locus_tag="ACHOUK_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFP9899035.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            complement(6016..6297)
                     /locus_tag="ACHOUK_003666"
     CDS             complement(6016..6297)
                     /locus_tag="ACHOUK_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFP9899036.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            6726..8312
                     /gene="pdeC"
                     /locus_tag="ACHOUK_003667"
     CDS             6726..8312
                     /gene="pdeC"
                     /locus_tag="ACHOUK_003667"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFP9899037.1"
                     /translation="MNHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIVPVNGYTIVSADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVPRNGYLYATVY
                     STKRPIAAIVATSYQRLIAHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQVMNPAEFIPLAEKEGMIEQV
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            complement(8315..8638)
                     /gene="soxS"
                     /locus_tag="ACHOUK_003668"
     CDS             complement(8315..8638)
                     /gene="soxS"
                     /locus_tag="ACHOUK_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFP9899038.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            8724..9188
                     /gene="soxR"
                     /locus_tag="ACHOUK_003669"
     CDS             8724..9188
                     /gene="soxR"
                     /locus_tag="ACHOUK_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFP9899039.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            9734..11083
                     /gene="ghxP"
                     /locus_tag="ACHOUK_003670"
                     /gene_synonym="yjcD"
     CDS             9734..11083
                     /gene="ghxP"
                     /locus_tag="ACHOUK_003670"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFP9899040.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            11234..12883
                     /locus_tag="ACHOUK_003671"
     CDS             11234..12883
                     /locus_tag="ACHOUK_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFP9899041.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(13037..>13527)
                     /gene="espX5"
                     /locus_tag="ACHOUK_003672"
     CDS             complement(13037..>13527)
                     /gene="espX5"
                     /locus_tag="ACHOUK_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector pentapeptide repeat protein EspX5"
                     /protein_id="HFP9899042.1"
                     /translation="LFRADLSFSKLDLINFKHADLSRVNLNKAILQNINLIDSKLFFT
                     RLTNTFLEMVICTDSNIANVNFNNANLNNCHFNCSVLTKAWMFNTRLYRVNFDEASVQ
                     GMGISILRGEENIPINSDTLVTLQKFFEEDCTSHTGMSQTENNTHEVAMKITADIMQH
                     AD"
BASE COUNT         3207 a         3279 c         3491 g         3550 t
ORIGIN      
        1 cctcggcaaa acagcaaagc gtgcaaatat acatacaatc tttcttaccc atgattttgt
       61 taactaaatc aataaaagcg tgaaaaactg tacttgcaat agaacttgat acttcttctt
      121 caggtctaca aaaatctttt gcaattttta actgtgtttc tatatataaa aagaaatctt
      181 tggcataaga aaaatctttg ttattttgta catctaactc aataggtaaa gatgtcgttt
      241 tgttgctatt cacatatgat atattcatat aatcatctcc ttcgggagta ataaaattaa
      301 tcaccattgt agggtagggt tctggtcaat cagaaatcat ctttataaac ttcgattgtt
      361 tttgtaatgc tgtatcatta agttcattaa atcgtacagc agataaatgt tctatcaaat
      421 ttcgctcatt tgcagagtat tcatcataat aaacgtaaaa ttaatgtatc cttacatcga
      481 gcattaacca ttttttacaa aaaaacgaca ggaacgtatt tactaggtta aatataatca
      541 tcctgctttt catcacaaaa accgcagata atccttcctt tcccctgtag ctggcgttat
      601 ggtcagatag tttttgcaac aaatctcaca ataaaaagtt tcaacatact gactatttag
      661 ggaaaaatat gcgcaagatc acacaggcaa tcagtgccgt ttgcttattg ttcgctctaa
      721 acagttccgc tgttgccctg gcctcatctc cttcaccgct taaccctggg actaacgttg
      781 ccaggcttgc tgaacaggca ccaattcatt gggtttcggt cgcacaaatt gaaaatagcc
      841 tcgcagggcg tccgccaatg gcggtggggt ttgatatcga tgacacggta cttttttcca
      901 gtccgggctt ctggcgcggc aaaaaaaact tctcgccaga aagcgaagat tatctgaaaa
      961 accctgtatt ctgggaaaaa atgaacaacg gctgggatga attcagcatt ccaaaagagg
     1021 tcgctcgcca gctgattgat atgcatgtac gccgtggtga cgcgatcttc tttgtgactg
     1081 gtcgtagccc gacgaaaaca gaaacggttt caaaaacgct ggcggataat tttcatattc
     1141 ctgccaccag catgaatccg gtgatctttg cgggcgataa accagggcaa aatacaaaat
     1201 cgcaatggct gcaggataaa aatatccgaa ttttttatgg cgattccgat aacgatatta
     1261 ccgccgcacg cgatgtcggc gctcgtggta tccgcattct gcgtgcctcc aactctacct
     1321 acaaaccctt gccacaagcg ggtgcgtttg gtgaagaggt gatcgtcaat tcagaatact
     1381 gacagagcgg gagagcgtga cactctcccg aatgctgttt ttttaatcac acctttatcc
     1441 tttcgctgtc ttgctgcaaa ctgattaaga gagttttatc aaggagcagc acatgtggta
     1501 tcaaaagacg ctcacgctta gcgccaaatc tcgtgggttt catctggtaa cggatgaaat
     1561 tctgaatcag ctggctgata tgccgcgcgt taacatcggc ttactgcatc tgttgctgca
     1621 acatacctcc gcctctctga cacttaatga gaactgcgat cccaccgtac gccacgacat
     1681 ggagcgtttt ttcctccgca ccgttcccga caacggaaat tatgagcatg actatgaggg
     1741 agcagacgat atgccttctc atatcaaatc ctcaatgctg ggaacatcgc ttgtattgcc
     1801 ggtgcataaa gggcgtattc agaccggcac ctggcaaggc atttggctgg gggaacatcg
     1861 catccacggc ggatcgcgtc gcatcatcgc gacactacaa ggggagtaaa aaatgaccat
     1921 ttcggagttg ctacaatatt gcatggcaaa accaggcgca gaacagagcg tgcataacga
     1981 ctggaaagcg acgcagatca aagtggaaga tgtactgttt gcgatggtga aagaagtaga
     2041 aaatcgccca gctgtttcgc tgaaaaccag cccggagctg gcggagctgc tacgtcagca
     2101 gcacagcgat gtgcgtccaa gccgccatct gaataaagcg cactggagca ccgtgtatct
     2161 cgacggttcg ctgccggatt cgcaaatcta ttatctggtg gatgcgtctt atcagcaggc
     2221 ggtgaattta ctgccggaag aaaaacgtaa attgctggtg caactctgaa aggaaaaggc
     2281 cgctcggaag gcggccttaa cgattacagc atcggcttga ggaagcgtgc cgtatgcgaa
     2341 gcttcgcact ccgcgacggt ttctggcgta ccggagacga ggatctcgcc gccgccactg
     2401 ccgccttccg gccccaggtc gacaatccag tcagcggttt taatcacgtc gagattgtgc
     2461 tcaatcacca caatggtgtt gccctgatcg cgcagtttat gcagtacgtc gagcagttgc
     2521 tgaatatcgg cgaagtgcag accggtggtc ggctcgtcga gaatatacag cgtctgcccg
     2581 gtgccgcgtt ttgacagctc acgcgccagc ttcacgcgct gggcttcacc accagaaagt
     2641 gtggttgcgg actgccccaa gcgaatgtac gtcagaccaa cgtccatcaa cgtttgcagc
     2701 ttacgcgcca gagctggcac cgcatcaaag aactcacgcg cctcttcgat ggtcatatcc
     2761 agcacttcgt ggatggtttt gcctttgtac ttaatctcca gcgtttcacg gttatagcgt
     2821 ttacctttgc actggtcgca cggcacgtaa atgtccggca ggaagtgcat ctccactttg
     2881 atcacgccat cgccctgaca ggcctcgcag cgtccgccac ggacgttaaa gctgaaacgt
     2941 cctggcgtat aaccacgcgc acgggattcc ggtacgcccg caaacagttc gcgcacaggc
     3001 gtaaacacgc cggtataggt cgccgggtta gaacgcggag tacgaccaat tgggctttgg
     3061 tcgatatcga ttactttgtc gaaatgctcc agtccctgaa tatcgcgata cggtgccggt
     3121 tcggcgatgg tcgcaccatt caactggcgt tgggcaatcg ggaacagtgt gtcgttaatc
     3181 agcgtcgatt taccggaacc tgaaacccct gtgatgcagg taaacagacc gactggcagc
     3241 gttagcgtca cgtctttcag gttgttgccg cgtgcgccag tcagcttaag tactttttcc
     3301 ggattcgccg gaacgcgttt cttcggcact tcaatcttgc gtttaccgct catgtactgt
     3361 ccggtcaacg actccggcac cgccataatc gcttccagcg gaccttccgc gaccacttca
     3421 ccgccatgta cacctgcacc aggaccgata tcgatcacat gatcagcggc gcgaatcgcg
     3481 tcttcgtcgt gctccaccac aatcacggta ttaccgagat cgcgcagatg gataagcgta
     3541 cccaacaggc gctcgttatc gcgctggtgc aggccgatag acggctcatc cagcacgtac
     3601 ataacgccaa ccaggcccgc accaatctgg ctcgccagac ggatacgctg ggcttcaccg
     3661 ccggaaagcg tttctgccga gcgggaaagc gtcaggtaat tcaggccgac gttaacgagg
     3721 aatttcaaac gatcgccgat ctctttaaga attttttccg caatcttcgc ccgctgacct
     3781 gcgagtttga gattgttgaa gaattccatc gcatgaccaa tgctcatgtc ggagatggcg
     3841 ggcagcggcg tattctcgac atacacgtgg cgcgcttccc gacgcagacg cgtcccttcg
     3901 cagctggcgc acgggcgatt gctgatgaac ttggctaatt cttcacgtac cgcactggat
     3961 tctgtctctt tataacggcg ctccatattg tgcagcacgc cttcgaacgg atgacgacgg
     4021 atggaggtat cgccacgatc gttcatgtat ttgaattcaa tgttttcttt gccagaaccg
     4081 tacaacacca ctttatgcac gttcgcgctc aggctgcccc acggcgcttc gacgtcgaac
     4141 ttatagtgat ctgccagcga tttcagcatc tggaaataat agaagttgcg gcgatcccag
     4201 ccacggatcg caccacctgc cagcgacagt tccggattct ggatcacgcg gtcaggatcg
     4261 aaatattgct gtacgccaag gccgtcgcag gtcgggcagg cacctgccgg gttgttaaac
     4321 gaaaacagtc gcggctccag ttcacgcata ctgtagccgc aaattgggca ggcgaagttg
     4381 gcggaaaaca gcagctcttc cgctttcggg tcgtccatat ccgccactac cgcggtacca
     4441 ccggaaagct ccagcgcggt ttcaaacgac tcggcaagac gttgggtaag atcgtcacgc
     4501 accttgaagc gatcaaccac cacttcaata gtgtgtttct tttgcagttc cagtttcggc
     4561 ggatcggaaa gatcgcagac ttcgccatca atacgagcac ggatgtaacc ctggcttgcc
     4621 aggttctcca gcgttttggt gtgttcgcct ttgcgctctt taatgattgg cgcgagcagc
     4681 atcagacgct tgccttccgg ctgcgacagc acgttatcca ccatctggct gacggtttgc
     4741 gccgccagcg ggacgtcgtg gtccggacag cgcggttcgc cgacgcgggc gtacaacaaa
     4801 cgcaaatagt cgtggatttc ggtgattgtc cccaccgtag aacgcgggtt atgagacgtc
     4861 gatttctgct caattgagat ggcaggagaa agcccctcaa tatgatcgac gtccggcttt
     4921 tccatcagtg acagaaactg ccgcgcgtag gcggaaaggg attcaacgta acggcgctgc
     4981 ccttcggcat ataaggtgtc gaaagcgagc gaggatttgc cagaacccga aagcccggtc
     5041 acgacaatga gcttgtcgcg ggggataacg aggttgatgt ttttgagatt atgggtgcgg
     5101 gcgccccgaa cttcgatctt atccattcac ctttcccgga ttaaacgctt ttttgcccgg
     5161 tggcatggtg ctaccggcga tcacaaacgg ttaattatga cacaaattga cctgaatgaa
     5221 tatacagtat tggaatgcat tacccggagt gttgtgtaac aatgtctggc caggtttgtt
     5281 tcccggaacc gaggtcacaa catagtaaaa gcgctattgg taatggtaca atcgcgcgtt
     5341 tacacttatt cagaacgatt ttttttcagg agacacgaac atggccagca gaggcgtaaa
     5401 caaggttatt ctcgttggta atctgggtca ggacccggaa gtacgctaca tgccaaatgg
     5461 tggcgcagtt gccaacatta cgctggctac ttccgaatcc tggcgtgata aagcgaccgg
     5521 cgagatgaaa gagcagactg aatggcaccg cgttgtgctg ttcggcaaac tggcagaagt
     5581 ggccagcgaa tatctgcgta aaggttctca ggtttatatc gaaggtcagc tgcgtacccg
     5641 taaatggacc gatcaatccg gtcaggatcg ctacaccaca gaagtcgtgg tgaacgttgg
     5701 cggcaccatg cagatgctgg gtggtcgtca gggtggtggc gctcctgcag gtggcaatat
     5761 cggtggtggt cagccgcagg gcggttgggg tcagcctcag cagccgcagg gtggcaatca
     5821 gttcagcggc ggcgcgcagt ctcgcccgca gcagtccgct ccggcagcgc cgtctaacga
     5881 gccgccgatg gactttgatg acgatattcc gttctgattt gtcattaaaa caataggtta
     5941 tattgtttta aggtggatga ttaaagcatc tgccagccat aaaaaagaag cctccgttat
     6001 ggaggcttct acgtatcagg tcaaaatcat tggccactgt ggcggtgtct gattcatctc
     6061 ttctatcatc accgactcga tgttgtgcca gatggcggtg atgtccgcca ttgtgatgcc
     6121 aagcaacccc attgcgaacc agcaggcgga aaccacgccc agaccgctgc tgatcactgc
     6181 aagcccgaca taatcagacc gacgaaaacg gatgttgagc gtgctggcca gaaacatcat
     6241 tacggcacta agaagttgcc agcgaagaag aaccacgcca gtggtgaggg tagccatcaa
     6301 acaattcctc tgaaaagagc cgatgccctg gacagcgcgg cgttgttcac gggaggcgtg
     6361 gtacactctg gctatcgcgg ggcttgcaga acacaaaaat gaaacactct gtttgtttca
     6421 ttaattttgt gaactatatc acaattgatt gtttgttagc cagattaggt cgtgactttt
     6481 attgctgtac agattatgtg gtttttcagt ggcattaagg gtatatcttc ccgccgcctc
     6541 tgtattcctg tagaaaatta attttgaata tcaatgaatt attttcatcc aagtgacgat
     6601 taaaaaggta tcaatttcaa atcaggcaaa agtgctattt ataccgtaag atttatctaa
     6661 agacgtcggt agccagggtt tcaccttgca atggccgggt ataaacaggc aggaaattga
     6721 tagcaatgaa tcatcgtgca cgacaccaat tactggcgtt gccgggcatt atctttttag
     6781 ttctctttcc catcattctt tcgctatgga ttgccttcct ttgggcaaaa tcagaagtga
     6841 ataatcagct ccgaaccttt gctcaactgg cgttggataa atccgagctg gtcattcgcc
     6901 aggcagattt agtgagcgat gcagctgaac gctatcaggg gcaagtttgc actccagccc
     6961 atcaaaagcg aatgttgaat attattcgtg gctatcttta tattaatgaa ttgatctatg
     7021 cccgtgataa tcatttttta tgctcatcgc tgatagtgcc tgtaaacggc tatacgattg
     7081 tatcggccga ttataagcgt gaacctaatg tttctatcta ttattaccgc gatacgcctt
     7141 ttttctctgg ctataaaatg acctatatgc agcggggaaa ttatgtggcg gttatcaacc
     7201 ctctcttctg gagtgaagtg atgtctgatg acccgacatt gcaatggggt gtgtatgata
     7261 cggtgacgaa aacctttttc tcgttaagca aagaggcctc agcagcaacg ttttctccgc
     7321 tgattcattt gaaggattta accgtaccaa gaaatggcta tttatatgcg acagtttatt
     7381 cgacaaaacg cccaattgca gccattgttg cgacttcata tcaacgtctt atagcccatt
     7441 tttataatca tcttattttt gcgttgcccg ccggtatttt ggggagtctt gttctgctat
     7501 tactctggct acgtattcga caaaactatt tgtctcccaa acgcaaattg caacgcgccc
     7561 tcgaaaaaca tcagctatgc ctttattacc agccaataat tgatatcaaa acagaaaaat
     7621 gtatcggcgc tgaagcgttg ttacgttggc ctggtgagca ggggcaagta atgaatccgg
     7681 cagagtttat tcctctggca gaaaaggagg ggatgataga acaggtaact gattatgtta
     7741 ttgataatgt cttccgcgat ctgggcgcgt accttgcaac acatgctgat cgctatgttt
     7801 ctattaatct gtcggcctcc gattttcata cgtcacggtt gatagcgcga atcaatcaga
     7861 aaacagagca atacgcggtg cgtccgcagc aaattaaatt tgaagtgact gagcatgcat
     7921 ttcttgatgt cgacaaaatg acgccgatta ttctggcttt ccgccaggct ggttacgaag
     7981 tggcaattga tgattttggt attggctact ctaacttgca taaccttaaa tcattgaatg
     8041 tcgatatttt gaaaatcgac aaatcgtttg ttgagacgct gaccacccat aaaaccagtc
     8101 atttgattgc tgaacatatc atcgagctgg cgcacagcct ggggttaaaa acgatcgctg
     8161 aaggcgtcga aactgaggag caggttaact ggctgcgcaa acgcggcgtg cgctattgcc
     8221 agggatggtt ctttgcgaag gcgatgccgc cgcaggtgtt tatgcaatgg atggagcaat
     8281 tacccgcgcg ggagttaacg cgcgggcaat aaaactacag gcggtggcga taatcgctgg
     8341 gagtgcgatc aaactgccga cggaaaacgc gggagaaggt ctgctgagag acataaccca
     8401 ggtccattgc gatatcaaaa atcggacgct cggtggtgcg caactcaacg gcggccagta
     8461 acagacggcg ttgacgaatg taatcgccaa gcgtctgatg cgtcaccgtg cggaacatcc
     8521 gttgcaggta ccactttgaa tagcctgatt tttttgcgac gacatcaatg ttaagcggct
     8581 ggtcaatatg ctcgtcaatc catgcgataa gatcctgaat aattttctga tgggacataa
     8641 atctgcctct tttcagtgtt cagttcgtta attcatctgt tggggagtat aattcctcaa
     8701 gttaacttga ggtaaagcga tttatggaaa agaaattacc ccgcattaaa gcgctgctaa
     8761 cccccggcga agtggcgaaa cgcagcggtg tggcggtatc ggcgctgcat ttctatgaaa
     8821 gcaaagggtt gattaccagt atccgtaaca gcggcaatca gcggcgttat aaacgtgatg
     8881 tgttgcgata tgtcgcaatt atcaaaattg ctcagcgtat tggcattcct ctcgcgacca
     8941 ttggtgaagc gtttggcgtg ttgcccgaag ggcatacgtt aagtgcgaaa gagtggaaac
     9001 agctttcgtc ccaatggcga gaagagttgg atcggcgcat tcatacctta gtggcgctgc
     9061 gtgacgaact ggacggatgt attggttgtg gctgcctttc gcgcagtgat tgcccgttgc
     9121 gtaacccggg cgaccgctta ggagaagaag gtaccggcgc acgcttgctg gaagatgaac
     9181 aaaactaaag cgccacaagg gcgctttagt ttgttttccg gtctttgtct ttctctctat
     9241 ctcgctggta cacaggaggg tttcccccga cgtcaacaca cctcattcga gcacgtggtg
     9301 gaggttccgg ttggtgttga tgctttaatt gtatgtcacc gacgtttctt cgccagtgta
     9361 aaagtatact ttttagccgc aatatttttg tcatctcaga cgatttttta tcgcaatcct
     9421 caacggtata cggctcgata acgctgcaat cttgcgcatc gacgataacg tttgcgcatc
     9481 aattgcctgg tttttcatcg tcaagacaat aaaagagaaa aaagcagcaa acttcggttg
     9541 aaaaagccgc tatgatcgcc ggataatcgt ttgctttttt taccacccgt tttgtatgcg
     9601 cggagctaaa cgtttgcttt tttgcgacgc agcaaattgt cgcaaacctg gagcaggaag
     9661 ataacgtttc gctggcaggg gattgtccgc cacgcatctt gacgaaaatt aaactctcag
     9721 gggatgttct cttatgtcta cgccatcagc gcgtaccggc ggttcactcg acgcctggtt
     9781 taaaatttca caacgtggaa gcactgtccg tcaggaagtg gttgccgggt taacaacgtt
     9841 tctggcgatg gtctactcgg ttatcgtcgt tccaggcatg ttgggtaaag cgggcttccc
     9901 gcctgcggca gttttcgttg cgacctgtct ggttgccgga ctcggttcta tcgtgatggg
     9961 tctgtgggct aatctgccgt tggcgattgg ttgcgccatc tccctgacgg cgtttaccgc
    10021 attcagcctg gtgctggggc aacatattag cgtacctgtc gcgctgggtg ccgtgttcct
    10081 gatgggtgtg ctgtttacgg taatttctgc cacgggtatc cgtagctgga ttttgcgcaa
    10141 cttgccgcac ggtgtggcgc acggcacggg gatcggtatc ggtctgttcc tgctgctcat
    10201 tgccgctaac ggtgtcggtc tggtgattaa aaacccgctt gatggtctgc ccgttgcgct
    10261 gggtgatttc gcgaccttcc cggtgattat gtcactggta ggtctggcgg tgatcatcgg
    10321 cctggaaaaa ctgaaagtcc ctggtggcat tctgctgacc attatcggta tctcaattgt
    10381 cggtttgatc ttcgatccta acgtccattt ctccggcgtt ttcgccatgc cttcattgag
    10441 cgatgaaaac ggcaattcac tgattggcag cctggacatt atgggcgcgc tgaatcctgt
    10501 agtcctgcca agcgttctgg cgctggtgat gacggcagta tttgatgcca ccggaactat
    10561 ccgcgctgtc gccggccagg cgaacctgct ggataaagat gggcagatca tcgacggcgg
    10621 gaaagcactg accactgact ccatgagcag cgttttctct ggcctggtgg gtgcagctcc
    10681 ggcagcggta tacatcgagt ctgcggcggg tacggcggcg ggcggtaaaa ccggtttgac
    10741 ggctatcacc gttggcgtgc tgttcctgct gattctgttc ctctctccgc tttcttacct
    10801 cgttccgggg tatgcaacgg ctccggcgct gatgtacgtt ggcctgctga tgctgagcaa
    10861 cgtggcgaaa atcgactttg ctgattttgt tgatgcgatg gcgggtctgg ttacggcggt
    10921 attcatcgtg ctgacctgta acatcgtaac aggcatcatg atcggcttcg cgactctggt
    10981 gattggtcgt ctggtttccg gtgaatggcg caagttgaac atcggtacgg tcgttatcgc
    11041 cgtggcgctg gtgaccttct atgcgggtgg ctgggctatc taatctttca tcggattctg
    11101 aaaacgggtg gcaatggctg cccgttttta ttttctccgc acattgtgtg agcttttgcg
    11161 atattctgaa aaaatgagaa ttcaggcata acgtctttct cagatcataa aaaacatcgc
    11221 aaacagggaa cgcatggaaa tcttcttcac catactgata atgaccctcg tggtctcgct
    11281 gtccggggtg gtcactcgtg tcatgccctt tcagatcccg cttccgctta tgcaaatcgc
    11341 catcggtgcg ctactggcgt ggccgacgtt tggtttgcat gtggagtttg atcctgaact
    11401 ctttttagtc ttgtttatcc cgccgttgct gttcgctgat ggctggaaaa cgccgacccg
    11461 tgaatttctc gaacatggtc gagagatttt cggcctcgcg ctggcgctgg tggtggtcac
    11521 cgtggtcggc attggcttcc ttatttactg ggtggtgccg ggcattccgc tgatccccgc
    11581 ctttgcgctg gcggcggtgc tttctccgac cgatgctgtg gcgctctccg ggattgttgg
    11641 cgaagggcgc atcccgaaaa aaatcatggg cattttgcag ggcgaagcgt tgatgaatga
    11701 cgcctccggc ctggtgtcgt tgaagtttgc cgtggcagtg gcgatgggga cgatgatctt
    11761 caccgtcggc ggtgcgacgg tcgaatttat gaaagtagcc attggcggta ttctcgccgg
    11821 ttttgtggtg agctggctgt acggtcgctc gctgcgattc ctcagccgct ggggcggtga
    11881 tgaacccgcg acgcagatcg tcctgctgtt cctgctgcca ttcgcttctt atctgattgc
    11941 cgaacatatt ggcgtttccg gcatcctcgc tgcggttgcc gccgggatga ccatcacccg
    12001 ctccggtgtg atgcgccgtg cgccgctggc aatgcgcctg cgtgcaaaca gcacctgggc
    12061 gatgctggaa tttgtcttta acggcatggt gttcctgctg ttaggtctgc agctgccggg
    12121 tattctggag acgtcgctga tggcagcaga aatcgaccct aacgtcgaaa tctggatgct
    12181 gtttaccgat attattctga tatatgcggc gctgatgctg gtccgtttcg gctggctgtg
    12241 gacgatgaaa aagttcagca accgcttcct gaagaagaag ccgatggagt ttggttcgtg
    12301 gaccacacga gaaatcctga tcgcgtcttt cgccggggtg cgtggggcga tcactttggc
    12361 cggtgtgctc tctatcccgc tgctcttgcc ggatggtaac gtcttcccgg cgcgctatga
    12421 gctggtgttt ctggcggctg gtgtcattct cttctcgctg tttgtcggcg tggtgatgtt
    12481 gcctattctg ctacaacaca ttgaagtcgc ggaccattcg caacaattga aagaagaacg
    12541 tattgcgcga gcggcaacgg cagaagtggc gattgtggcg atccagaaaa tggaggagcg
    12601 tctggcggcg gataccgaag agaatatcga taaccagctg cttacggagg tcagttctcg
    12661 cgtcattggt aacctgcgtc gtcgcgccga tggacgtaac gacgttgaaa gttccgtgca
    12721 ggaagagaac cttgagcgtc gcttccgtct ggcggcattg cgttctgaac gtgctgaact
    12781 gtaccacctg cgcgccacgc gggagatcag caacgaaacg ctgcaaaaat tactgcacga
    12841 tctcgatttg cttgaagcgt tgctaattga ggaaaatcag taagccgaaa gcgacgtaac
    12901 caggcccgag gtaatctgaa aaaggtgcaa tcttttgcac ctttaacagg ttagctcggg
    12961 ctaacattca tgattctaaa acaaataagt tatctctctt ttaaagtcat ttcattggct
    13021 gttcttcgac ttaatctcaa tctgcgtgtt gcataatatc tgcagtaatc ttcatagcta
    13081 cttcatgagt attattctca gtttgtgaca tgccagtatg agaggtacaa tcttcttcaa
    13141 agaatttctg tagtgttacc agggtatcac tattaatggg aatattctcc tccccacgga
    13201 gaatggaaat gcccattccc tggacgctag cctcatcgaa attaacccga tagagacggg
    13261 tattaaacat ccaggctttt gttaaaacag aacagttgaa atggcaattg tttaaattgg
    13321 cattattaaa attaacatta gctatattag aatcggtgca tataaccatt tcgaggaacg
    13381 tatttgttag ccgtgtaaaa aagagtttac tatcaattaa gtttatattc tggaggattg
    13441 ctttgttcag attgacgcga gacagatcag catgtttaaa gttaattaaa tcaagtttag
    13501 agaagctaag gtcggctcta aataaat
//
LOCUS       DAZKIP010000114        13478 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZKIP010000114 DAZKIP010000000
VERSION     DAZKIP010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.115"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            89..1147
                     /locus_tag="ACHOUK_003673"
     CDS             89..1147
                     /locus_tag="ACHOUK_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFP9899043.1"
                     /translation="MNKKIWFMEGLSSQRDIIQGVKSFAQKNNFAITVFASHRNERNE
                     ILSVADYSLTEPEDPQKRLQFIQETIQTYGIHHIHTGRNSQWFEEHRSAIEPTGATLT
                     TGATGVDWLTLADEKVTFAQFMEQKGLPVVPSWRVNTLAELKTHLAAPPFTDSPVCVK
                     PVTGIYGMGFWRFDDSASPMAVFNHPEHRLVSPQQYIAAASAAESFKPLVLMPYLPGP
                     EFSVDILADKGEILAAVGRRKEGAIQYLVNEGSAWELACDCARVMKADGLVNVQTRND
                     VNGNPVLLETNMRPSGGVGYTLHSGVNLPGLFAAFKLGLMSEDMVRQSAKNTFSPVAV
                     RSITDVIAYPESLSNLLN"
     gene            1157..2299
                     /locus_tag="ACHOUK_003674"
     CDS             1157..2299
                     /locus_tag="ACHOUK_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HFP9899044.1"
                     /translation="MSDILEDMIYRRTLSCGTIQVTRDQGEVSLDDLFDIAERRNPKR
                     AFLFVSKVLGRHIPVPPSVMRQAYRQLASQFPSTLTGPVLFIGMAETAVGLGAGVFDE
                     VRHQHRESVYLTSTRHPVDGTLLCEFKEEHSHATDHLIYLPDDEEKRRRVTNARTLVL
                     IDDEATTGNTFINLLSALRNTGKLQHIEQVIAVTLTDWSGNALSERTPLPVTSVSLVS
                     GQWGWTPLPDAPVPDMPKVNVTSRGEWDIQGKQSWGRLGMLAPAADLGHEVSVHKGER
                     ILVLGTGEFVWEPFLLAERLEAAGAQAFYGSTTRSPVAVGYAIESAISFTDNYGLGIP
                     NFVYNVAHQQFDRILVCTETPAESIDTQLLKALAEVAPVVEIVTYE"
     gene            2292..3065
                     /locus_tag="ACHOUK_003675"
     CDS             2292..3065
                     /locus_tag="ACHOUK_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899045.1"
                     /translation="MNKPVVLSDLDDTLFQTRRKMVDELALEPFRTGAVDRTLNPRSF
                     MTEEQSMLVDWLLEQAELIPVTARGTEEISRVRIPFHSWAITTHGAVILTPEGKPDEE
                     WKAHMLGQLAPYQEKLTSMQRLITEMMDAKGINAWARLNFEYGETAVYMVMKHRDSTR
                     LDELNAIADEIETVFPTEGFYIHRNSNNVAWLPTPVEKGLAVSWLLEKLRAERGVFPV
                     IGLGDSLSDHRFMKLCSWFGIPRQSQFADAISQRIFGEN"
     gene            3067..4146
                     /locus_tag="ACHOUK_003676"
     CDS             3067..4146
                     /locus_tag="ACHOUK_003676"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HFP9899046.1"
                     /translation="MQPHETFTGSYQPGDVEFLLKPVVIEMTPVEKKEELIQSGKKHY
                     SDMLSQEPAPTQWHLDLFHRALDRGAERLAKEVTQLAIALAERFGDEPIVLASLVRAG
                     VPLGVMLHQALRDMGKTSWHYGISIIRDRGIDGAALDVIEERHGTSGIVFVDGWTGKG
                     AITGELVRALKDRPGYPEQPRLVVLADPCGCSWLAASDDDWLIPFGIMGAPVSGLISR
                     SVWSSEGLHGCMVCEHLSEFECSRMLADTVAHFRKKLTPSSLAPLSWNTESARVLWQT
                     SRDVIAFLADEFKVDSVNRIKPGIAEATRAVLRRVPDHVFVRSIDDPDVALLVGLARE
                     KGIVVTEMGGTLGQYRAVTIIKKVL"
     gene            4146..5102
                     /locus_tag="ACHOUK_003677"
     CDS             4146..5102
                     /locus_tag="ACHOUK_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HFP9899047.1"
                     /translation="MKNRLSPWNLGATLYMPATREDIADAVLHGKIPGLRSLVICLED
                     AVSEADIPVALKNLEHLLHELSNSMSSLGKNDWPLVFIRPRHAEMGRWLKAHYDLSAV
                     DGFVLPKFTLSSLAEWWDIMVGTHLCMMPTLETEDVFDVVQMRELATRLEEHPCHDRI
                     IALRIGGNDLMNVVSLRRPRDLTLYDSPMGYVIKMLVSVFGPRDFALTAPVCEHIDDH
                     AVMARELALDMAHGLVGKTAIHPGQIEVIQNALMVTQGEHSDALRILNSTQAVFKSQG
                     AMCEPATHRRWAAGILDRARFYGLQNEQSADGIRLLTVTQHH"
     gene            5113..6321
                     /locus_tag="ACHOUK_003678"
     CDS             5113..6321
                     /locus_tag="ACHOUK_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HFP9899048.1"
                     /translation="MGFRFRKSINIIPGVRLNLSNGAPSLSVGPRGASVSFGSRGTYA
                     NLGLPGTGLSYRTRLDRAARSRGENRTATDPGLRQALEQKAAELMSAVTAIRNIHELT
                     PDPKTGISWAELEAVYLHNRTSPFQVPAPVRPEKPDYLVLPEKPAESEGISFLGKWFE
                     SESAKAERHAENLRRWQQELIDVERENTLRQHRYQQQRTAWAEQYANWKFEAEEHEKR
                     LATAQADARQQFRTDAAFFESYLAGVLAETEWPRETLVAFEVKPELSAVLLDVDLAEI
                     EDFPDKIYGVNARGTELTEKAMTQKAVRENYAHHVHGCLFRLVGIVLHTLPFDNVIVS
                     GFTQRVSKRTGYLEDEYILSCKCTRSQMSSVNFAGIKHIDPVEALGDQPVIRKMSSTF
                     IFQPIEPLTL"
     gene            6339..6806
                     /gene="terW"
                     /locus_tag="ACHOUK_003679"
     CDS             6339..6806
                     /gene="terW"
                     /locus_tag="ACHOUK_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance protein TerW"
                     /protein_id="HFP9899049.1"
                     /translation="MQLNTRQARIFKLANLLGTGKPVSAADIITSLECSEPTLTRALK
                     ELRESYSAEIKYSKAGHSYHLVNPGQLDKKTLRRMNEALAQNAELKTGESTGKVVLDK
                     DKKTAVSLSLRMRILRKIDRLAALSDSTRSEAVEKLALHSVDELIKEYSAKKS"
     gene            7067..7396
                     /locus_tag="ACHOUK_003680"
     CDS             7067..7396
                     /locus_tag="ACHOUK_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9899050.1"
                     /translation="MSKPRWTLDQKKHHVAAWRASGLTREQYCELYDIPFKSLRQWPQ
                     DVAKAEKRARAPEIIPVSVSGSSGMTDGRPLSDEPVTLFLPGGIRMCCQPSQLTDVFR
                     ALRHADA"
     gene            7383..7724
                     /gene="tnpB"
                     /locus_tag="ACHOUK_003681"
     CDS             7383..7724
                     /gene="tnpB"
                     /locus_tag="ACHOUK_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309368.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9899051.1"
                     /translation="MLTPDNVFLVVKPVDMRRGIDTLTQYVQNELNAAWHDGAAFVFT
                     NKVRSRIKVLRWDKHGVWLCTRRLHRGSFRWPRKGDATWHLTQDEFHWLVFGVDWQQV
                     KGHDLAKWVYQ"
     gene            7806..8636
                     /locus_tag="ACHOUK_003682"
                     /pseudo
     CDS             7806..8636
                     /locus_tag="ACHOUK_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(8667..10280)
                     /locus_tag="ACHOUK_003683"
     CDS             complement(8667..10280)
                     /locus_tag="ACHOUK_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element IS682 family transposase"
                     /protein_id="HFP9899052.1"
                     /translation="MSRKYLIRITELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     ARAEEKIEEEEREIEHLRAQIEKLRRMLFGTRSEKLQREVEQAEAQLKQREQESDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGSELDYLGEVSAEQLE
                     LVSSALKVIRTVRVKKACTKCDCIVEAPAPSRPIERGIAGSGLLARVLTGKYCEHLPL
                     YRQSEIFARQGIELSRALLSNWVDACCQLMTLLNDTLYRYVMNTRKVHTDDTPVKVLA
                     PGRKKAKTGRIWTYVRDDRNAGSSEPPAVWFAYSPDRQGKHPVQHLRPFRGILQADAF
                     SGYDRLFSAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEI
                     RGLPESERLAVRQQRSKVLLTSLHEWMVEKNGTLSKKSRLGEACSYVLNQWDALCYYS
                     DDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAY
                     LRHILSVLPEWPSNRVDELLPWNVVLTNK"
     gene            complement(10311..10661)
                     /gene="tnpB"
                     /locus_tag="ACHOUK_003684"
     CDS             complement(10311..10661)
                     /gene="tnpB"
                     /locus_tag="ACHOUK_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9899053.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLDENPFSGHLF
                     IFRGRRGDTIKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            complement(10658..11083)
                     /locus_tag="ACHOUK_003685"
     CDS             complement(10658..11083)
                     /locus_tag="ACHOUK_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9899054.1"
                     /translation="MEQKILSSEPRRSFSNEFKLQMVKLASQPGASVARIAREHDIND
                     NLLFKWLRLWQNERRISRRLPVTTSSGAGVELLPVEITPDEQKEPMAALTPLLSTPSQ
                     STVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            11137..11888
                     /locus_tag="ACHOUK_003686"
                     /pseudo
     CDS             11137..11888
                     /locus_tag="ACHOUK_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT         3242 a         3543 c         3508 g         3185 t
ORIGIN      
        1 atcatattat gattgacatg tagcttagac actcatagac tcacttctca atcataaatt
       61 tgcatggcgt tccggataat taaaaaaaat gaacaagaaa atttggttta tggaaggttt
      121 atcctcccag cgagatatta ttcagggggt aaaatcattt gcacaaaaaa ataattttgc
      181 cattaccgtt tttgcctccc accgtaacga aagaaatgaa atcctttccg ttgccgatta
      241 ttctttgact gaacctgaag atcctcaaaa acgtcttcag tttatccagg aaaccattca
      301 gacctacggc atccaccata ttcatactgg ccgtaacagc cagtggtttg aagaacaccg
      361 ttcagccatt gaaccgaccg gtgccaccct tactaccggt gcaacgggcg tcgactggtt
      421 aactctggct gacgaaaaag ttacttttgc tcagtttatg gagcaaaagg gtctcccggt
      481 cgtaccatcc tggcgggtga atacgctggc agaattaaag acacacctcg cggccccgcc
      541 gttcactgac agcccggtat gcgtgaagcc ggtgacgggt atctatggca tgggattctg
      601 gcgctttgat gacagtgctt cgcctatggc cgtctttaat catcccgaac atcgtctggt
      661 cagtccgcaa cagtatattg cagcagcatc agctgctgag tcgtttaaac cccttgtttt
      721 gatgccgtac ctgccaggcc cggaattttc cgtcgatatc ctcgcggata agggcgaaat
      781 actcgcagcc gtgggacgcc gtaaggaagg ggctatccag tatctggtaa acgaaggaag
      841 cgcctgggaa ctggcgtgtg actgcgcccg tgttatgaag gccgacgggc tggtgaatgt
      901 tcagacgcga aacgatgtga atggcaaccc ggtgctgctt gaaaccaaca tgcgtccgtc
      961 agggggggtg ggttataccc ttcacagcgg tgtgaacctt cctgggttat ttgctgcctt
     1021 taagctcggt ctgatgtctg aagatatggt acgccagagc gctaaaaaca ccttttctcc
     1081 ggttgcggtg agatccatta cggatgtaat tgcatacccg gaatcactct ctaaccttct
     1141 gaattaaggc gtatttatgt ctgatatcct cgaagatatg atttatcgcc gtaccctctc
     1201 atgcggtacg attcaggtaa cccgtgacca gggtgaagtt tcgctcgatg acctgtttga
     1261 catcgctgaa agacgtaacc cgaaacgcgc ctttctgttt gtcagcaaag tactcggcag
     1321 gcacattccc gtgccaccct cagtcatgcg gcaggcctac agacagcttg ccagccagtt
     1381 cccctcgaca ctgacaggac ccgtactgtt tatcggtatg gctgaaaccg ccgttgggct
     1441 tggggccggt gtatttgatg aagtgcgcca ccagcatcgt gaatctgtct atctgacctc
     1501 tacccgtcac ccggttgatg gcacgttgct ttgcgagttc aaggaagaac acagccacgc
     1561 caccgatcat ctgatctatc tgccagatga tgaagagaaa agacgtcgtg ttaccaacgc
     1621 acgaacgctg gttttgattg atgacgaggc aaccaccggt aataccttta ttaacctgct
     1681 ttctgccctg cgtaataccg gcaagcttca acatattgaa caggttatcg ccgtaacgct
     1741 gaccgactgg agtggcaatg ccctgtcgga gcgcaccccc ttgcccgtca cttcggtttc
     1801 gcttgtaagc ggtcagtggg gatggacccc attacctgat gcacctgtcc cggacatgcc
     1861 gaaagtcaac gtaacttcac gaggagaatg ggacatccag gggaaacagt catggggccg
     1921 actggggatg ctcgcacctg cggccgatct cggccatgag gtctccgtcc acaaggggga
     1981 acgtattctg gttctcggga ccggggaatt cgtctgggag ccgttcctgc ttgctgaacg
     2041 gctcgaagct gccggagcac aggcatttta tggatcgacc acccgctccc ctgtcgccgt
     2101 tggttatgcc attgagtccg ccatttcctt tacggataac tacgggctgg gcatccccaa
     2161 ttttgtctat aacgtcgccc accagcagtt tgaccgcatt cttgtgtgta ctgagacacc
     2221 cgcagaaagt attgacacgc agcttcttaa agcgctggct gaggttgcgc ccgtcgtgga
     2281 gattgttacc tatgaataaa cccgttgttc tgagcgacct tgacgacacg ctctttcaga
     2341 cccgtcgtaa aatggtggac gagctggccc tggagccatt ccgtaccggt gccgttgatc
     2401 gcaccctgaa tccacgaagc tttatgacgg aagaacagtc catgttggtg gactggctcc
     2461 tggagcaggc tgaactcata cccgttaccg cacggggaac tgaagaaatc agccgcgtac
     2521 ggatcccttt ccactcctgg gcaatcacaa cgcacggggc cgtcattctc acgcctgaag
     2581 gcaaacccga cgaggagtgg aaggcccata tgctcggcca gctggctccc taccaggaaa
     2641 agctgacatc gatgcagcgt ctgatcactg aaatgatgga cgcaaaagga atcaatgcct
     2701 gggcaaggct gaacttcgaa tacggtgaaa cggcggttta tatggtgatg aaacaccgcg
     2761 acagcacccg ccttgacgag ctcaatgcca ttgcagatga gatagaaacg gtgtttccga
     2821 ccgagggctt ctacatccac cgcaacagta ataacgtggc ctggcttccc accccggttg
     2881 agaaagggct ggcagtcagc tggctgcttg aaaaacttcg ggctgaacga ggagttttcc
     2941 ccgtaattgg tttgggcgac agcctgagcg atcatcgttt tatgaaactg tgcagctggt
     3001 ttggcatccc gcgtcagagt cagtttgcag atgccatttc acagcgaatt tttggagaaa
     3061 attaaaatgc agccacatga aacatttacc ggctcatacc agcccggtga cgtggaattt
     3121 ctgctaaagc cggttgtcat tgagatgacg ccggttgaga aaaaagaaga actgattcag
     3181 tccgggaaga aacattattc ggacatgctc agccaggagc cagcgcctac acaatggcat
     3241 cttgatttgt ttcaccgggc gctggatcgg ggcgcagaga ggcttgcaaa agaagtcaca
     3301 cagctcgcta ttgctctggc cgaacgcttc ggtgatgagc ccattgtact ggccagtctt
     3361 gtcagggctg gcgttccgct cggcgttatg ctgcaccagg ccctgcgtga catggggaaa
     3421 acctcatggc attacggtat cagcattatc cgggatcgtg gaattgacgg tgcagccctc
     3481 gacgtcattg aagagcggca tggtaccagc ggtattgtct ttgttgacgg atggacaggt
     3541 aaaggcgcaa ttaccggaga gcttgtacgc gcactgaaag atcgcccggg ctatcctgag
     3601 cagccgcgac tggttgtcct ggccgatccc tgtggctgct cctggcttgc agccagtgat
     3661 gatgactggc tcattccttt tggcatcatg ggggcgccgg tatcaggcct gatctcccgg
     3721 tcagtatggt cctctgaagg attacacggg tgcatggttt gcgagcatct cagtgaattc
     3781 gaatgcagcc ggatgcttgc cgataccgtc gctcatttcc gtaagaagtt aacaccgtca
     3841 tccctcgctc ccctgagctg gaatacggaa tcagcccggg tcttatggca gacaagtcgc
     3901 gacgttatcg cgttcctggc cgatgaattt aaagtggaca gcgtcaatcg tattaaaccc
     3961 ggtattgctg aagcaacccg ggctgtatta cgtcgggtac cggaccatgt atttgtgcgt
     4021 tctattgacg acccggacgt tgccttgctt gtagggcttg ctcgtgaaaa gggaatagtt
     4081 gttacagaaa tggggggaac cctcggccag tatcgggctg taaccattat caagaaggta
     4141 ctctgatgaa aaatcgtctt tctccctgga atctgggagc cacgctatac atgcctgcaa
     4201 cacgggaaga tattgctgat gccgtcctgc acgggaaaat cccgggcttg cgctctctgg
     4261 tgatttgcct ggaggatgct gtcagtgaag cagacatccc cgtcgccctc aaaaatctgg
     4321 agcacctgct gcacgagctc agtaacagca tgagtagcct gggtaaaaat gactggcctc
     4381 tggtgttcat tcgcccccgg catgccgaaa tgggcagatg gttaaaagcg cattatgatc
     4441 tttccgctgt cgacgggttt gtgctgccta aatttaccct cagttcgtta gctgaatggt
     4501 gggatatcat ggtcgggact cacctgtgca tgatgccaac gctggaaaca gaagatgtct
     4561 ttgacgtggt tcagatgcgc gagctggcta cccgtctgga ggaacatccc tgccacgacc
     4621 gcattattgc gcttcgaatc ggcggcaacg atcttatgaa cgttgtgtcg cttcgccgtc
     4681 cccgggacct gacgctgtat gacagcccga tgggctacgt catcaaaatg ctcgtttcgg
     4741 tcttcggccc gcgtgatttt gccctgaccg cgcctgtatg tgagcatatt gacgatcatg
     4801 ccgttatggc cagagagctg gctcttgata tggcacacgg gcttgttggg aaaacggcca
     4861 ttcacccggg tcagatagag gtcattcaaa acgcgctgat ggtcactcag ggtgagcatt
     4921 ctgacgctct gcggatcctg aactctaccc aggccgtgtt taagtcgcag ggagcaatgt
     4981 gtgaacccgc cacacatcgc cgctgggcgg ctggcattct ggacagggct cgtttttatg
     5041 ggttacagaa cgagcaaagc gctgatggaa tcagattact taccgtgacc cagcatcatt
     5101 aaggaaatca taatggggtt caggttcaga aagtcgatca acattattcc tggcgttcgc
     5161 ctcaacctga gtaacggtgc accgagcctg agtgtcgggc cgagaggtgc ttccgtttct
     5221 tttggtagcc gggggaccta tgccaatctg ggcttgcccg gtaccgggct gagttaccgt
     5281 acccggcttg accgggccgc gcgttccaga ggtgaaaacc ggacggcaac cgacccgggg
     5341 ctcagacagg cgcttgagca gaaagccgct gaactcatgt cagcggtaac cgcaatccgt
     5401 aatattcacg agctgacgcc ggatccaaaa acaggcatca gctgggcaga gctggaagca
     5461 gtatacctgc ataacagaac gtcgcctttt caggttccgg caccggtgcg tccagaaaag
     5521 ccagactacc ttgtattgcc ggaaaagcct gccgagagcg aaggcattag ttttttgggt
     5581 aaatggtttg aatcggaatc agctaaagct gagcgccacg ccgaaaatct tcgccggtgg
     5641 cagcaggagc tgattgatgt ggagcgtgag aatacccttc gacagcaccg gtaccagcaa
     5701 caacggacgg cctgggccga acagtatgca aactggaagt ttgaagctga agaacatgaa
     5761 aaacggctcg ccacggctca ggcagatgcc cggcagcagt tccggacaga cgccgcgttt
     5821 ttcgaatcat acctggcggg tgtgctggca gaaactgaat ggccgcgtga aacgcttgtt
     5881 gcatttgaag taaagccgga gctatcagca gtcctgctgg acgttgattt agctgagatt
     5941 gaagatttcc ctgataagat ttacggcgtt aatgcccggg gaacggagct gacggaaaaa
     6001 gccatgacgc aaaaagccgt acgcgaaaac tatgcccacc acgtccatgg ctgcttgttc
     6061 cgcctggtcg gtatcgtttt acatacgcta cctttcgaca acgtgattgt gtcaggcttt
     6121 acgcaacggg tcagtaagcg gaccggctat ctggaggatg agtatatcct gtcctgcaaa
     6181 tgcactcgca gccagatgtc gtcagtaaat tttgcaggca taaaacacat tgatccggtt
     6241 gaagcgttag gcgatcaacc ggttattcga aagatgagca gtaccttcat ttttcagcct
     6301 attgaaccac taacccttta agcaggctaa atcttacgat gcaattaaat accagacagg
     6361 cccggatttt taagcttgcc aacttactgg gcaccggcaa gcccgtttcc gctgccgaca
     6421 ttataaccag tctggaatgc tctgagccga cattaacccg ggccctcaaa gagctacgcg
     6481 agtcatattc tgcggagatc aaatacagca aggcaggtca ttcctatcat ctcgttaatc
     6541 cgggccagtt ggataaaaag accctccgcc ggatgaatga agcgctcgca cagaatgctg
     6601 aactgaaaac cggtgagtcc acagggaagg tcgtactcga taaggataaa aaaacagccg
     6661 tgtcattatc gctacgcatg cggattctaa gaaaaattga ccgtctggct gcgctgagtg
     6721 actcgacccg aagtgaagcg gtagagaagc tggctctgca ctccgttgat gaactgataa
     6781 aagaatacag cgctaaaaag tcctgacgct aaatctcaga ccggggtgaa aactccggtt
     6841 ttgtcatatt tcacctctgt attggcgtga tgcaaaatgg catctgaatc gatcatggtt
     6901 tggcatccat ttccgatcac tgtttggcat cacaatcgat cacgatttgg catgcttccg
     6961 ctcattgatt ggcatcctgg tgtcgttacg gggaagtcat cttcacaggg atcttcagtc
     7021 agttagcatt accgacatcc attcaccaac ttaccggaga taatcaatgt caaaaccccg
     7081 atggacgctt gatcagaaaa aacatcacgt tgccgcatgg cgcgccagcg gccttacccg
     7141 tgaacagtac tgtgaacttt acgacattcc gtttaaatcc ctgcgccagt ggccgcagga
     7201 tgtcgcaaaa gccgaaaaac gagccagagc accagaaatt attcctgttt cagtatctgg
     7261 ctcttccgga atgactgatg gcagaccatt gtcggatgag cctgtcactc tcttcctgcc
     7321 cggtggtatc cggatgtgct gccagccttc tcagttaacc gatgttttcc gggctcttcg
     7381 ccatgctgac gcctgataac gtattcctgg tcgtaaaacc cgtcgatatg cgccggggta
     7441 tcgacacact gacacagtac gtccagaacg aacttaacgc tgcatggcat gacggtgctg
     7501 cttttgtctt caccaataaa gtccgctccc gcataaaagt tctccggtgg gacaaacatg
     7561 gcgtatggct gtgtacccga cgactgcacc ggggaagctt ccgctggccc cggaaaggtg
     7621 atgccacatg gcatctcaca caggatgagt ttcactggct ggtctttggg gttgactggc
     7681 aacaggtgaa agggcatgac ctggcaaaat gggtatacca gtaagaaccc gaagatgctc
     7741 agagcaacgc aataactcaa tgaaatataa tgaatatcca tgcgtgatgt catataatcc
     7801 accgcatgga tatctcgctt ctcagtacca ccagtgaccc tgaacagctt cgcgctctgg
     7861 caattgccat ggtgcaaaaa gtcatggctg agaatgcaga acttcagaac cgtatccgca
     7921 tcctggaaga gcagatgaag ctcgcacgcc agcagcgttt cggtaaaaaa tgcgaatcgc
     7981 tggccgggat gcagcgttcc ctgttcgaag aggatgttga cgcggatatc gcagaaatct
     8041 cggcgcatct ggacaaactg cttcctcaga ccggggacga agaaaaaaca acaacacgtc
     8101 ctgttcgtaa gcccttgcct tcacctcttc cccgtgctga aaaggttatc ccgccagctg
     8161 aggaacgttg tcctgactgt gatgcccccc tgcacttcat tcgcgatgaa gtcagcgaga
     8221 agctggaata tattccggca caggtcgtgg tcaaccgcta tatccgcccg cagtacagct
     8281 gtccctgctg tgagaaagtg ttcagcggaa aaatgccggc acatatcctt ccgaaaagtg
     8341 ctgttgagcc atcggtcatc gcacaggttg tcatcagcaa atataccgac catctgcccc
     8401 tgtatcgcca gcagcacatt ttctcccgaa tgggggtgga gctccccgtc agtacaatgg
     8461 cggatatggt cggtgttgct ggcgctgcac tggccccgct ggcaaaattg ttacgccacg
     8521 agcttctgac acgggatgtt attcatgcag atgaaacgtc cctgaggctc cttgataccc
     8581 ggaaaggcgg taaatcctgc tccggctggc tgtgggctta cgtcagcgga gaaagagtaa
     8641 gcgtcaacgg agcaccgtat tgacgcttat ttattggtga gtactacgtt ccatggcagg
     8701 agttcatcaa ctcggttgga aggccattct ggcagtacgc tcaggatatg gcgcagatac
     8761 gcttccggat cgataccgtt cagacggcag gtgccgatca gcccgtacaa cagtgctcca
     8821 cgctcgccgc cgtgatcgct gccaaagaac ataaagtttt tctttccgag acagactgca
     8881 cgaagcgctc tttccgcagc attattatcc gcctccgcca gaccgtcatc actgtaataa
     8941 cagagggcat cccactgatt cagtacatag ctgcacgctt cgcccagtct ggattttttc
     9001 gacagcgtac cattcttctc caccatccat tcatgcagtg acgtcagtaa cactttgctt
     9061 cgctgctgcc tgacggcaag acgctctgac tccggtaatc cccgtatttc atcctcgatg
     9121 gcgtacagtt cactgattcg cttcagggct tcttctgccg tcgcactttt gctgctgatg
     9181 tatacatcgt ggatttttcg ccgggcatgg gcccagcacg caacttctgt cagtgcacca
     9241 ccttcacgtt ctgcactgaa cagccgatcg taaccgctga acgcatccgc ctgcaggata
     9301 ccccggaagg gacgaaggtg ttgtaccgga tgttttccct gcctgtctgg tgagtaggcg
     9361 aaccagaccg ccggtggctc tgatgagccc gcattccggt catcccggac atacgtccag
     9421 atgcgtcctg tttttgcctt ttttctgccc ggtgccagca cttttactgg tgtgtcgtca
     9481 gtgtgaacct tgcgggtgtt catcacgtaa cggtacaggg tatcattcag cagagtcatt
     9541 aactgacaac atgcgtcaac ccagttggag agtaatgccc ggctcagttc gatgccctgg
     9601 cgggcaaaga tttcactctg acgatacagt ggcaggtgtt cgcagtattt tcccgttaac
     9661 acgcgggcaa gtaatccgga gcccgcgatg ccgcgctcta tcgggcggga cggcgctggc
     9721 gcttcaacta tacagtcaca ttttgtacag gcttttttta cccgaacagt gcggatcact
     9781 ttcagggcgc tgctcaccag ttccagctgc tcagcactga cttcccccag ataatccagc
     9841 tcactgccac actccgggca acaactttct tcaggctcca gacggtgtat ttcacgggga
     9901 agatgtgccg gtaacggacg acgatgacgt gactgtcgca actggcgggg aacctgcggg
     9961 tcatcctcac gcccactgta acgatcgctt tcctgttcgc gttgtttcag ttgggcctca
    10021 gcctgttcaa cctcacgctg cagtttttca gaacgggtac cgaacaacat ccggcgcagt
    10081 ttttctatct gggccctcag atgttctatt tcccgctcct cctcttcgat cttttcctcg
    10141 gcacgtgcca gtgcagagcg caggaaggcc tccgtctctt caaccagact cagttgctga
    10201 tctttctgac ggagggcttc agcctgctca gagagcagcc tttccagctc agtgatacga
    10261 atgaggtatt tccgactcat gaccgttttt ataatccggc catgacattt ttacaacatt
    10321 gtcagtgcat taaggcggga tgttttgggc tgacgccagt ccagtttatc gaggagcatt
    10381 gccagctgcg agcgggtaat ggatacctta ccgtcacgca ccgcaggcca gataaactgg
    10441 ccttcctcca gacgtttggt gaacaggcac agaccatcag catcagccca cagaatttta
    10501 atcgtgtcac cccgtcggcc gcgaaagata aacaggtgac cggagaaggg gttctcatcc
    10561 agcacatgtt gtacctgttc tcccagaccg ttgaaggatt tacgcatatc agtaacgccg
    10621 gcaaccagcc agattcgagt gcctgatggg agcgagatca tcgtcccctc ccggtcagtt
    10681 cacggatcaa caccgtgagc agctctggtg aaggattttc cagcgtcatg ttaccgtggc
    10741 ggaactcaac tttacaggaa ctggcactga ctgtgctttg tgaaggagtg gataaaagcg
    10801 gagtaagagc cgccataggc tctttctgct catcaggcgt tatctcaaca ggtaataatt
    10861 caacgccagc gccagaagag gttgttaccg gaagacgccg cgatatacgc ctttcgttct
    10921 gccagagcct gagccatttg aacaggaggt tatcattgat atcgtgttcc cgggcaatac
    10981 gggcaacaga ggctcctggt tgtgaagcca gtttaaccat ttgaagttta aactcatttg
    11041 aaaatgatct gcggggttct gaggataata ttttctgttc cataacaggt gtccactagt
    11101 tgaaaaggtg ggcacctacg ttaccaatac tggcttaatg gctacatacg gcggtcagtt
    11161 tacgcttacg agaaagaagc ggtccgccgg tcgtctgttt tgactcgcag accggacggg
    11221 ccctcagata tcctgaggcc tggctgcagg gctggtgtgg cggtacgctg gtgagtgacg
    11281 gatacagcgt gtacaaatct ctggcggata atgaccccgg tattacatct gcctgttgct
    11341 ggagccacgc aaggcgcggt ttcgcaaatc tgtataaggc cagcagggag ccccgtgcag
    11401 ccatggcgct gagaaaaatc gcgggtcttt atcgcattga gaagttcatc cgtgaacgtc
    11461 ctgtggaaaa aatacggcaa tggcgacaac ggtattcccg gccgatagtg aatgacctgt
    11521 ttgcctggcc tgaagagcag gagccgtgct gtccgcccga cggaccacta aacaaagcga
    11581 ttaattatat cctgaaccgg cgcgatgaac tgagctgctt cctgagtgac ggagccgtgc
    11641 ccctggacaa caatatctgc gagcgtgcta tccgcccggt ggtcatgggg cggaaagcat
    11701 ggttattcgc cggttcactg atggccggga accgcgcggc acagataatg agccttctgg
    11761 aaacggcaaa acgtaatggt ctggagccgc atgcctggct gacagacgtt ctgacgcgtc
    11821 taccggagtg gccggaagac cgactggaag agttgctgcc ccttgaggga tttaccttct
    11881 ccgggtgagt gatacctgcc atcaggtgtt cgtgcagcgg gccataaccc gcagtcggga
    11941 gttgaacgcc tgacgacagg aaatgagcca gagcagcgac cacgcgggct gacagtgagt
    12001 cagaacctga atcagcccgg agccattccc ggggctgtac ttcatcacct gccatcagta
    12061 cccgtgtcag tacttcgcga cctgacgcaa ttacccgact cagttcttca gaacccgcct
    12121 ccgataccag cctggcggtc gcccctgtgc aacaaaataa atcaaccatc gtcagacggt
    12181 gatcaatcgt atgcgctgcc agtggctcgc ctgaaaaatg cgtcaatatg agttcgccgg
    12241 aacaatacct cagttcttca gaacccgcct ccgataccag cctggcggtc gccccggcgc
    12301 aacagaataa atcaaccatc gtcagactgc gatcattcgt atgcatcgct gatgatgcgc
    12361 ccgaaaaacg cgtcaatatg agttcgccgg aatattactg acgacacggt ttgtagttac
    12421 gttccttcgg tgagtgagta cctgtgcgtc agggtaacgc aatcagttca ggtttagcgg
    12481 ggtgtcaagt cggtttggct aaccgaccgc agaattaact gtacctttaa aggtcgcccc
    12541 cgatatatct gcctgtcaga catatcgccg attcggtatc tcactacata aaactcctta
    12601 aaaaacaatg cgattctgtt ggtacgaagc tttcagtaag tcagtccgga gaagcgccag
    12661 tacgatacag aatgaggttg aaaaaaacgt tcacgtctgg tcacttcttc aaatgttaaa
    12721 aaacatctcg taaagagcca gtttatgcgg gttcgttgag tagacgtgcg agcgtcactt
    12781 tgtcagcttt aacgctgacg aaagtgacgc ccgcacagct tcaaactcca catgcacacg
    12841 caagcgacca aatttcaaat ggtcttatgc cgtgcgcaca gtcataagaa gcgcatgcgg
    12901 aaagtcagaa aatatcttaa gacgcagatt ttaaaggcgt ttttatgcca gtctgccggg
    12961 aatacacagt agtccggttg tccggaagca ccacggtaat ccgaaggaaa aatcaacgta
    13021 cctcaatcac cgtagtgata tccgttccca gaggctaaac ggatgtacca ccataaaggc
    13081 atcctctttc aggctacgag gatattgcaa tcacccgaag gcgtttctct cagattgctg
    13141 aaacattggc tggcagtcac ccgaaggcgt ccctctcaga ctgctggaac attggctggt
    13201 cgcaaaagcg acagttaatg caaatacagg ttaacaaaaa gggaaatctg ccgtcaaccc
    13261 aaccatatga acatgttgat ttcattatga atgtgatgtt ctaatataaa ttaatcagta
    13321 acttcataaa aagaatataa gaaacagata atgcatgtat ttacacacat atcagcacaa
    13381 tgatagatta ccaccccaga aacacgataa aggtataatt atggaatttt ccgttatttt
    13441 tttctgggtt atcactggtc ttattaccat cttattct
//
LOCUS       DAZKIP010000115        13456 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZKIP010000115 DAZKIP010000000
VERSION     DAZKIP010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13456
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.116"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..197)
                     /locus_tag="ACHOUK_003687"
     CDS             complement(<1..197)
                     /locus_tag="ACHOUK_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899055.1"
                     /translation="MNEYVYSARHNAFFPVDMIDKYKSEGWDLSDAKEVNQNIISEFM
                     AEPPQGKIRIAGDDGLPAWAD"
     gene            complement(218..628)
                     /locus_tag="ACHOUK_003688"
     CDS             complement(218..628)
                     /locus_tag="ACHOUK_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9899056.1"
                     /translation="MSGKDVAGLLAYLSLGEAAKRDVGTGENQIPDMASFASGDGWMK
                     LPNGKILQYGRGAVTPTLSTQTMRITFSIPFPKKADCAMLTHSGDGGAPLGAGRGFVM
                     TAEGPTLTGFNSAYRTSSTSDTVSMNYSWWAVGE"
     gene            complement(1028..1417)
                     /locus_tag="ACHOUK_003689"
                     /pseudo
     CDS             complement(1028..1417)
                     /locus_tag="ACHOUK_003689"
                     /inference="COORDINATES: protein motif:HMM:NF023985.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            complement(1414..2025)
                     /locus_tag="ACHOUK_003690"
     CDS             complement(1414..2025)
                     /locus_tag="ACHOUK_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFP9899057.1"
                     /translation="MSDSRLLPTGSSPLEVAAARACAEIEKTPVSIRELWNPDTCPAN
                     LLPWLAWSFSVDRWDDKWPEATKRAVIRDAYFIHCHKGTIGAIRRVVEPLGYLIEVRE
                     WWQLNEEPGTFRIVVGVLEQGITEEMYQELERLVADAKPASRHLTGLAISLSTTGNIF
                     AGAGCYHGDALTVYPYTPEAIIVGGDYFPASAIHLIDNLRVNA"
     gene            complement(2018..2926)
                     /locus_tag="ACHOUK_003691"
     CDS             complement(2018..2926)
                     /locus_tag="ACHOUK_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HFP9899058.1"
                     /translation="MPIIDLNQLPAPDVVEELDFETILAERKATLISLYPEDQQEAVA
                     RTLTLESEPLVKLLEENAYRELIWRQRVNEAARAVMLACAAGNDLDVIGANYNTTRLI
                     ITPADDSTIPPTPAVMESDTDYRLRIQQAFEGLSVAGSVGAYQYHGRSADGRVADISV
                     TSPSPACVTISVLSRENNGVASEDLLAVVRNALNGEDVRPVADRVTVQSAAIVEYQIN
                     ATLYLYPGPESEPIRAAAVKKLEAYITAQHRLGRDIRLSAIYAALHVEGVQRVELTAP
                     LADIVLNSTQASFCTEYSVVTGGSDE"
     gene            complement(2931..3278)
                     /locus_tag="ACHOUK_003692"
     CDS             complement(2931..3278)
                     /locus_tag="ACHOUK_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFP9899059.1"
                     /translation="MTARYLGMNRSDGLTVTDLEHISQSIGDILRTPVGSRVMRRDYG
                     SLLALMIDQPQTPALELQIKVACYMAVLKWEPRVTLSSVTTERSFDGRMTVTLTGQHN
                     DTGQPLSLTIPVS"
     gene            complement(3275..3910)
                     /locus_tag="ACHOUK_003693"
     CDS             complement(3275..3910)
                     /locus_tag="ACHOUK_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFP9899060.1"
                     /translation="MNTLANIQELARALRNMIRTGLVVETDLNAGRCRVQTGGMCTDW
                     LQWLTCRAGRSRTWWAPSVGEQVLILAVGGELDTAFVLPGIYSGDNPAPSASADALHI
                     RFPDGAVIEYEPETSALTVSGIKTASVTASDSVTATVPVVMVKASTRITLDTPEVVCT
                     NRLITGTLEVQKGGTMRGNIEHTGGELSSNGKVLHTHKHPGDSGGTTGSPL"
     gene            complement(3977..4429)
                     /locus_tag="ACHOUK_003694"
     CDS             complement(3977..4429)
                     /locus_tag="ACHOUK_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFP9899061.1"
                     /translation="MNEFKRFEDRLTGLTESLSPSGRRRLSAELAKRLRQSQQRRVMT
                     QKAPDGTPYAPRQQQSVRKKTGRVKRKMFAKLITSRFLHIRASPEQASMEFYGGKSPK
                     IASVHQFGLSEENRKDGKKIDYPARPLLGFTGEDVQMIEEIILAHLDR"
     gene            complement(4422..4889)
                     /locus_tag="ACHOUK_003695"
     CDS             complement(4422..4889)
                     /locus_tag="ACHOUK_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9899062.1"
                     /translation="MLKPDSLRRALTDAVTVLKTNPDMLRIFVDNGSIASTLAASLSF
                     EKRYTLNVIVTDFTGDFDLLIVPVLAWLRENQPDIMTTDAGQKKGFTFYADINNDSSF
                     DISISLMLTERTLVSEVVGALHVKNIPEPPPPEPVTRPMELYINGELVSKWDE"
     gene            complement(4852..5097)
                     /gene="lysC"
                     /locus_tag="ACHOUK_003696"
     CDS             complement(4852..5097)
                     /gene="lysC"
                     /locus_tag="ACHOUK_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979584.1"
                     /note="LysC is an Rz1-like component of a phage lytic
                     system, substantially overlapping although not fully
                     embedded in the gene for the Rz-like LysB component;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1-like lysis system protein LysC"
                     /protein_id="HFP9899063.1"
                     /translation="MLCAGCTSAPPAQTPVIVANACPKVSLCPMPGSDPQTNGDLSAD
                     IRQLENALARCASQVKMIKHCQDENDAQTRQPAQGAD"
     gene            complement(4997..5422)
                     /gene="lysB"
                     /locus_tag="ACHOUK_003697"
     CDS             complement(4997..5422)
                     /gene="lysB"
                     /locus_tag="ACHOUK_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585210.1"
                     /note="The gene for this Rz-like phage lysis system
                     protein may overlap extensively with the gene for the
                     other spanin subunit, the Rz1-like protein in the outer
                     membrane; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz-like lysis system protein LysB"
                     /protein_id="HFP9899064.1"
                     /translation="MSKLMIVLVVLLSLAVAGLFLAKHENASLRASLDRANNVASGQQ
                     TTIIMLKNQLHVALTRADKNEMAQVALRQELENAAKREAQREKAITRLLNENEDFRRW
                     YGTDLPDAVRRLHQRPACADASDCRQRLPESEPLPDAGQ"
     gene            complement(5410..5835)
                     /locus_tag="ACHOUK_003698"
     CDS             complement(5410..5835)
                     /locus_tag="ACHOUK_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNZ54_00345 family protein"
                     /protein_id="HFP9899065.1"
                     /translation="MKKLSLSLMLHVSLALMLALSLIYPQSVAVSFVAAWAILATVIC
                     VVAGGVGVYATEYVLERYGRELPPESLAVKIVTSLFLQPVPWRRRAAALVVMVATFIS
                     LVAAGWIFTALIYLVASLFFRLIRTACRQRFEGREPCQS"
     gene            complement(5850..6347)
                     /locus_tag="ACHOUK_003699"
     CDS             complement(5850..6347)
                     /locus_tag="ACHOUK_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HFP9899066.1"
                     /translation="MPVINTHQNIAAFLDMLAVSEGTANHPLTKNRGYDVIVTGLDGK
                     PEIFTDYSDHPFAHGRPAKVFNRRGEKSTASGRYQQLYLFWPHYRKQLALPDFSPLSQ
                     DRLAIQLIRERGALDDIRAGRIERAISRCRNIWASLPGAGYGQREHSLEKLVTVWRTA
                     GGVPA"
     gene            complement(6347..6628)
                     /locus_tag="ACHOUK_003700"
     CDS             complement(6347..6628)
                     /locus_tag="ACHOUK_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFP9899067.1"
                     /translation="MTAEEKSVLSLFMIGVLIVVGKVLAGGEPITPRLFIGRMLLGGF
                     VSMVAGVVLVQFPDLSLPAVCGIGSMLGIAGYQVIEIAIQRRFKGRGKP"
     gene            complement(6632..6835)
                     /locus_tag="ACHOUK_003701"
     CDS             complement(6632..6835)
                     /locus_tag="ACHOUK_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFP9899068.1"
                     /translation="MKTFALQGDTLDAICVRYYGRTEGVVETVLAANPGLAELGAMLP
                     HGTAVELPDVQTAPVAETVNLWE"
     gene            complement(6835..7344)
                     /locus_tag="ACHOUK_003702"
     CDS             complement(6835..7344)
                     /locus_tag="ACHOUK_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFP9899069.1"
                     /translation="MMTLIIPRKEAPVSGEGTVVIPQPAGDEPVIKNTFFFPDIDPKR
                     VRERMRLEQTVAPARLREAIKSGMAETNAELYEYREQKIAAGFTRLADVPADDIDGES
                     IKVFYYERAVCAMATASLYERYRGVDASAKGDKKADSIDSTIDELWRDMRWAVARIQD
                     KPRCIVSQI"
     gene            complement(7444..8187)
                     /locus_tag="ACHOUK_003703"
     CDS             complement(7444..8187)
                     /locus_tag="ACHOUK_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HFP9899070.1"
                     /translation="MTSPAQRHMMRVSAAMTAQRDAAPLRHATVYEQMLVKLAADQRT
                     LKAIYSKELKAAKKRELLPFWLPWVNGVLEQGKGAQDDILMTVMLWRLDTGDIAGALE
                     IARYALKYGLTMPGKHRRTPPYMFTEEVALAAMRAHAAGESVDTRLLTETLALTATAD
                     MPDEVRAKLHKITGLFLRDAGDAAGALAHLQRATQLDCQAGVKKEIERLERELKPKPE
                     PQPKAATRAPRKTRSVTPAKRGRPKKKAS"
     gene            complement(8191..9264)
                     /locus_tag="ACHOUK_003704"
     CDS             complement(8191..9264)
                     /locus_tag="ACHOUK_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFP9899071.1"
                     /translation="MRQETRFKFNAYLSRVAELNGIDAGDVSKKFTVEPSVTQTLMNT
                     MQESSDFLTRINIVPVSEMKGEKIGIGVTGSIASTTDTAGGTERQPKDFSKLASNKYE
                     CDQINFDFYIRYKTLDLWARYQDFQLRVRNAIIKRQSLDLIMAGFNGVRRAETSDRSS
                     NQMLQDVAVGWLQKYRNEAPARVMSKVTDEEGHTTSEVIRVGKGGDYASLDALVMDAT
                     NNLIEPWYQEDPDLVVIVGRQLLADKYFPIVNREQDNSEMLAADVIISQKRIGNLPAV
                     RVPYFPADAMLITKLENLSIYYMDDSHRRVIVENPKLDRVENYESMNIDYVVEDYAAG
                     CLVEKIKVGDFSTPTKVTAEPGA"
     gene            complement(9323..10177)
                     /locus_tag="ACHOUK_003705"
     CDS             complement(9323..10177)
                     /locus_tag="ACHOUK_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFP9899072.1"
                     /translation="MAKKVSKFFRIGVEGDTCDGRVISAQDIQEMAETFDPRVYGCRI
                     NLEHLRGILPDGIFKRYGDVAELKAEKIDDDSALKGKWALFAKITPTDDLIAMNKAAQ
                     KVYTSMEIQPNFANTGKCYLVGLAVTDDPASLGTEYLEFCRTAKHNPLNRFKLSPENL
                     ISVATPVELEFEDLPETVFTALTEKVKSIFGRKQASDDARLNDVHEAVTAVAEHVQEK
                     LSATEQRLAEMETAFSALKQEVTDRADETSQAFSRLKNSLDHTESLTQQRRSKATGGG
                     GDALMTNC"
     gene            10351..12123
                     /locus_tag="ACHOUK_003706"
     CDS             10351..12123
                     /locus_tag="ACHOUK_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HFP9899073.1"
                     /translation="MTITTDTTLLHDPRRQAALLYWQGFSVPQIAAMLQMKRPTVQSW
                     KQRDGWDSVAPISRVEMSLEARLTQLIIKPQKTGGDFKEIDLLGRQIERLARVNRYSQ
                     TGNEADLNPNIANRNKGGRRKPKKNFFSDEAIEKLEQIFFEQSFEYQLHWYRAGLEHR
                     IRDILKSRQIGATFYFSREALLRALKTGHNQIFLSASKTQAYVFREYIIAFARLVDVD
                     LTGDPIVLGNNSAKLIFLGTNSNTAQSHNGDLYVDEIFWIPNFQVLRKVASGMASQSH
                     LRSTYFSTPSTLAHDAYPFWSGELFNRGRASAAERVEIDVSHNALAGGLLCADGQWRQ
                     IVTIEDALKGGCTLFDIEQLKRENSADDFKNLFMCEFVDDKASVFPFEELQRCMVDTL
                     EEWEDYAPFAANPFGSRPVWIGYDPSHRGDSAGCVVLAPPVVAGGKFRILERHQWKGM
                     DFATQAESIRKLTEKYNVEYIGIDATGLGVGVFLLVRSFYPAARDIRYTPEMKTAMVL
                     KAKDVIRRGCLEYDVSATDITSSFMAIRKTMTSSGRSATYEASRSEEASHADLAWATM
                     HALLNEPLTAGISTPLTSTILEFY"
     gene            12123..13157
                     /locus_tag="ACHOUK_003707"
     CDS             12123..13157
                     /locus_tag="ACHOUK_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001752368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9899074.1"
                     /translation="MSKKKGKTPQPAAKKMTASAPKMEAFTFGEPVPVLDRRDILDYV
                     ECISNGRWYEPPVSFTGLAKSLRAAVHHSSPIYVKRNILASTFIPHPWLSQQDFSRFV
                     LDFLVFGNAFLEKRYSTTGKVIRLETSPAKYTRRGVEEDVYWWVPSFNEPTAFAPGSV
                     FHLLEPDINQELYGLPEYLSALNSAWLNESATLFRRKYYENGAHAGYIMYVTDAVQDR
                     NDIEMLRENMVKSKGRNNFKNLFLYAPQGKADGIKIIPLSEVATKDDFFNIKKASAAD
                     LLDAHRIPFQLMGGKPENVGSLGDIEKVAKVFVRNELIPLQDRIREINGWLGQEVIRF
                     KNYSLDTDNG"
BASE COUNT         2902 a         3935 c         3611 g         3008 t
ORIGIN      
        1 atatctgccc acgcaggcag cccatcatct ccggcaatac ggatttttcc ttgtggcggt
       61 tcagccataa actcactgat aatattttga tttacttcct tagcgtctga taaatcccat
      121 ccctctgatt tatatttatc aatcatatcc acagggaaaa aagcattatg ccttgcgcta
      181 taaacatatt cgttcatata aatcacccta aataaaatta ctcaccaaca gcccaccaac
      241 tgtaattcat cgataccgtg tcgctggttg atgacgttct gtaagcagaa ttaaagccgg
      301 ttaacgttgg gccttctgca gtcatcacga accctcgccc agcgcctaaa ggcgcaccgc
      361 catcaccaga atgagtaagc atggcgcagt ccgctttttt ggggaaaggg atgctgaatg
      421 taattctcat tgtttgcgtc gataatgtcg gcgtaaccgc accacgacca tattgcagga
      481 ttttaccgtt gggtaatttc atccatccat caccactggc aaaagaggcc atgtccggta
      541 tctgattttc ccctgtcccc acatcccgtt ttgccgcttc tcccaaacta aggtatgcga
      601 gaagaccggc aacatccttt ccactcaaat gagtcagcgt ctcatccagt ggctgcttac
      661 ctgacagcgc attgttaatg gtggtgctga atttcgggtc attgttaatg gccgcggcaa
      721 tttctttcag tgtgtccagc gtgtctggcg cgccgttaat cagagcggta atagcggtct
      781 gaacaaactc agtggtcgca atccgcgtgg tgttatttcc tgcggcaggc gttggcgctt
      841 ttggttctcc ggtaaatgtc ggattatgtt tctgcgcata ctgggtatga ggatcctgtg
      901 cggcaatgtg gtttctcatc tggtcatcca catacagcct taattccagg actttatcat
      961 ccacgtattt acgggtcgcc agtaccaccg acgggtcgat tttcagcgtg atggcttcgg
     1021 tattcgtgac aaccagaatc atgcggatag tctgggtacg accgctgcct tcctgtaact
     1081 gcggtttgta cgtttccggg cagttcgcca ccgcaatgag tacgccttca tcatcataaa
     1141 gcccaatctc acggatccag aatccgccct cgttctcagg gatgatttgc tccgcaataa
     1201 tctggctctg gttgttcggg tcaacactca gaagattcag cggcgcgatg cgtttctggt
     1261 taataagttt tgtctgtgcc gggtcaggtg tcggcaagac accatttgca tcaccaacgg
     1321 ccatttgcgt cagattcagc ttactgccga gcatcgtcgc gttagccagc cgtgccgcgc
     1381 cctgattagt cagaatggcg tagtatttca ctgtcatgcg tttactctca ggttatcaat
     1441 taaatgaatg gccgaggccg ggaaataatc ccctccgaca ataatggcct ccggggtgta
     1501 gggataaacc gtcagggcgt cgccgtgata gcatcccgca ccggcaaaaa tgttgccggt
     1561 tgtacttaaa ctgatagcca gtcccgtcag atggcggctt gcaggttttg catcagcaac
     1621 gagacgctcc agctcctgat acatttcctc ggtaataccc tgctcaagca cgccaacaac
     1681 gatgcggaac gtccccggct cctcgttgag ctgccaccac tccctcacct caatcagata
     1741 gccgagcggc tccaccacac ggcgaatcgc accaatagtg cccttatggc agtgaatgaa
     1801 atacgcatcg cggataacag cgcgttttgt cgcttccggc cacttatcat cccagcggtc
     1861 aaccgaaaat gaccacgcca gccacggcag cagatttgcc ggacaggtat ccgggttcca
     1921 cagctcacga atactgaccg gcgttttttc aatttccgca caggctcttg cggcggcgac
     1981 ttcaagcggt gatgagccgg tcggcagcag gcgcgaatca ctcatccgag cctccggtca
     2041 cgacgctgta ttcggtgcag aaagacgcct gcgtactgtt gagcacaatg tcagccagag
     2101 gtgcagtcag ttcgacacgt tgcacgcctt ccacatgcaa agcggcataa atggcagaca
     2161 gacggatgtc gcgcccaagc cggtgctgtg ccgtgatgta cgcttccagt tttttcacgg
     2221 cagcagcgcg gatgggttcg ctttcgggac ctgggtaaag gtaaagcgtg gcgtttatct
     2281 gatattcaac aatggcggca gactgcacgg tcacgcggtc ggccaccggc ctgacgtcct
     2341 cgccattaag ggcgttacgc accacggcca gcaggtcttc ggatgcgacg ccgttatttt
     2401 cacgtgacag cacggagata gtgacgcagg ccggagacgg actggtgaca gagatatccg
     2461 cgacacgccc gtcagcactg cgaccatgat actgataggc tcccaccgac ccggcgacgc
     2521 ttaagccctc aaacgcctgc tgaatacgca gacgataatc ggtgtcagac tccatcactg
     2581 ccggtgtcgg cgggatagtc gaatcatctg ccggggtgat aatcaggcgt gtggtgttgt
     2641 aattggcacc aatcacatca aggtcattac cggcggcaca ggccagcatc accgcccgtg
     2701 cggcctcatt cacacgctga cgccagataa gctcacgata agcattttcc tccagaagtt
     2761 tgacgagagg ctcggattcc agcgtcaggg tacgggcgac cgcctcctgc tggtcttccg
     2821 ggtaaaggga aatcagtgtc gccttgcgtt cggcaagaat ggtttcaaag tccagctcct
     2881 cgaccacatc cggtgcgggt agctggttca ggtcgataat cggcatggtt tcaactcaca
     2941 gggatggtta acgaaagtgg ctggccggtg tcgttgtgct ggccggttaa cgtgaccgtc
     3001 attcgcccgt caaaactgcg ctcagtagtg acggatgaca gggtgacgcg gggttcccat
     3061 ttcagcaccg ccatgtaaca ggcgacctta atctgcaact caagcgccgg ggtctgcggc
     3121 tggtcaatca ttaatgccag caacgagccg taatcacgac gcatcacccg tgagccgacc
     3181 ggtgtacgca ggatatcgcc gatactctgg ctgatatgct caaggtcagt gacagtcagg
     3241 ccatcactgc gattcattcc gagataacgc gctgtcataa aggactcccg gttgtgccgc
     3301 cgctgtcgcc ggggtgttta tgggtatgca gtaccttacc gtttgatgag agttcaccac
     3361 cggtgtgttc aatgttgccg cgcatcgtcc cgcccttctg tacttccagc gtgccggtaa
     3421 tcagcctgtt ggtgcagacc acctccggtg tgtccagggt gatgcgggtt gacgctttca
     3481 ccatgaccac cggcaccgtg gcagtaacag aatcagaagc cgtcacgctg gccgttttaa
     3541 ttccgcttac cgtcagtgca ctggtttcag gttcatactc aatcaccgcc ccgtcaggga
     3601 aacggatatg cagggcatcc gccgacgcag acggcgcggg gttatcgccg gaataaatcc
     3661 ccggcagaac aaacgctgtg tcaagttcac cgcccacggc cagaatcagc acctgctccc
     3721 ccacggaagg tgcccaccat gtgcgcgaac gcccggcacg acaagtcagc cactgaagcc
     3781 agtcggtgca catgccgccg gtctgcacac ggcagcgacc ggcgttaagg tcggtttcga
     3841 cgacaaggcc ggtgcgaatc atgttgcgca gtgcgcgcgc gagttcctgg atatttgcga
     3901 gagtgttcat aacgggaagg atgccgccgg gtcataccgg cggcaatgtg acgatgaggt
     3961 gtcaggaatg gcacaactaa cggtcgaggt gagccaggat aatctcttca atcatctgca
     4021 catcctcacc ggtaaagccg agcagaggac gcgccggata atcaattttc ttaccgtctt
     4081 tccggttttc ttccgacaga ccgaactgat gcacgctggc gattttcggt gacttcccgc
     4141 cgtaaaactc cattgatgcc tgttccgggc tggcacggat atgcaaaaaa cgactggtaa
     4201 taagtttcgc aaacattttt cgcttaacgc gaccggtctt ttttctgacg ctctgctgct
     4261 ggcgtggcgc gtagggtgtg ccgtccgggg ctttctgtgt catcacccga cgctgctgac
     4321 tctgccgcag acgtttcgcc agttcggcgc tcagtcgtcg acgccctgac ggtgacagcg
     4381 attcagtcag tccggtcagc cggtcttcaa aacgcttaaa ctcattcatc ccacttgctc
     4441 accagttcgc cattgatata aagctccatc gggcgggtga ccggctccgg cggcggaggt
     4501 tccgggatat tcttcacatg cagtgcgccg accacctcac tgaccagcgt gcgctcggtc
     4561 agcatcaggc tgatgctgat atcaaagctg ctgtcattgt tgatgtctgc ataaaacgtg
     4621 aagccctttt tctggcctgc gtcggtggtc atgatgtcgg gctgattttc ccgcagccac
     4681 gccagcaccg gcacgatgag caggtcaaaa tcaccggtaa agtcggtcac aatgacattg
     4741 agcgtgtaac gcttttcgaa tgacagcgac gccgccagtg tggaggcaat actcccgtta
     4801 tccacgaata tccgcagcat atcggggtta gttttcagca ccgtgacggc atcagtcagc
     4861 gccctgcgca ggctgtcggg tttgagcatc gttttcgtcc tgacagtgtt taatcatttt
     4921 tacctggctg gcacagcgtg ccagcgcgtt ctcaagctgc cggatatcag cacttaaatc
     4981 gccgttcgtc tgcgggtcac tgcccggcat cgggcaaagg ctcactttcg ggcaggcgtt
     5041 ggcgacaatc actggcgtct gcgcaggcgg ggcgctggtg caaccggcgc acagcatcag
     5101 gcaggtcagt gccgtaccag cggcgaaaat cttcgttttc attgagtaac ctcgtgatgg
     5161 ctttctcgcg ctgtgcttca cgcttcgcgg cgttctccag ctcctgacgc agtgccacct
     5221 gcgccatctc gtttttgtct gccctggtga gggcaacatg aagctgattt ttcagcatga
     5281 tgatggtcgt ctgctgccca ctggcgacat tgtttgccct gtccagcgag gcgcgcaggc
     5341 tggcgttttc atgcttcgcc agaaacagac cggccaccgc cagtgataac aacacaacca
     5401 gcacaatcat cagctttgac atggttcccg cccctcaaaa cgctgacggc aggccgtacg
     5461 tatcagccgg aaaaacagcg acgccacaag ataaatcagc gcggtaaaaa tccacccggc
     5521 ggcgaccagc gagataaacg tcgccaccat caccaccaga gccgccgccc gtctgcgcca
     5581 cggcaccggc tgcaaaaaca gcgacgtgac aatcttcacg gccagcgatt ccggcggcag
     5641 ctcccgcccg tagcgttcca gtacatactc tgtggcatac accccgacac caccggcaac
     5701 cacacagata accgtcgcca gaatcgccca ggcagcgaca aaactgacgg ccacgctctg
     5761 cggataaatc agggacagtg ccagcatcag cgccagcgac acgtgcagca tcagtgaaag
     5821 ggataatttc ttcatggtgt ttactccgtt taagccggta cgccgccagc ggtacgccag
     5881 acggtgacca gtttttccag tgaatgctca cgctgaccgt aaccggcacc cggcagggac
     5941 gcccagatat tgcgacagcg tgaaatggca cgctcaatgc gtcccgcccg gatgtcatcc
     6001 agtgcaccgc gttcgcggat caactgaatg gcgagcctgt cctgtgacaa cggactgaaa
     6061 tccggcaggg caagctgttt gcggtaatgc ggccagaaca ggtaaagctg ctgatagcga
     6121 ccggaggccg tggatttttc accgcgacgg ttaaacacct tcgccggtcg gccatgcgcg
     6181 aacgggtggt cactgtagtc ggtgaaaatt tccggctttc cgtccagtcc ggtgactatc
     6241 acgtcatagc cccggttttt cgtcagcgga tgattcgccg tcccttcgga cacggccagc
     6301 atgtcgagaa aggcggcgat attctgatgc gtgttaatta ccggcattac ggtttccccc
     6361 tgcccttaaa gcggcgctga atggcaatct caatcacctg ataaccggcg atacccagca
     6421 tggagccgat accgcacacc gcaggcagtg acaggtcagg aaactgcacc agaacaacac
     6481 cggcaaccat cgagacaaaa ccaccgagca acatgcgccc gataaacaga cgcggggtga
     6541 tgggttcacc accggcaagc accttgccga caacaatcag cactccaatc atgaaaagcg
     6601 acaggacgct tttttcttct gctgtcatgc gttactccca cagattgaca gtttcagcca
     6661 cgggcgcggt ctgaacgtcg ggcagttcga cggcggtgcc gtgcggcagc atcgcaccca
     6721 gttcagccag tcccggattt gcggcgagca cggtctcaac cacgccctca gtgcgcccgt
     6781 aataccggac acaaatggcg tcgagcgtgt cgccctgtag cgcaaaggtc ttcatcagat
     6841 ttgactcacg atgcagcgcg gcttgtcctg gatacgtgcc accgcccagc gcatatcccg
     6901 ccacagttca tcaatggtgc tgtcaatgct gtcggccttc ttgtcgccct tggcactggc
     6961 atccacgccg cgataacgct cataaagcga tgcggtcgcc atcgcacaca cggcgcgctc
     7021 gtagtaaaaa actttgatgc tttcaccgtc gatgtcgtcc gccgggacgt ccgccagacg
     7081 cgtaaaaccg gcagcaattt tctgttcgcg gtactcgtac agctccgcat tcgtttccgc
     7141 catgcctgac ttgatggcct cacgcagacg ggcgggggcg acggtctgct caaggcgcat
     7201 acgttcccgg acgcgcttcg ggtcgatatc gggaaaaaag aacgtgtttt taatcaccgg
     7261 ctcgtcgcct gccggttgcg ggatgaccac cgtaccctca ccggacacgg gagcctcctt
     7321 tcgcggaata atcagcgtca tcatgactac ctctgaaaag tcgggcggtg gacgccggtg
     7381 cagtgtcagg tgattcaccc tcactgaccg gcgtgccgcc ctggcgcggg gcgcattcgg
     7441 ttgttaacta gctttctttt tcgggcgtcc acgttttgcc ggtgtcacgc tccgggtctt
     7501 acgcggggcg cgggtggccg ctttgggctg cggctccggc ttcggtttca gctcccgctc
     7561 cagtcgttca atctcttttt tgacgcctgc ctgacagtcg agctgtgtcg cacgttgcag
     7621 gtgagccagc gcaccggcgg catcaccagc gtcacgcaga aacagaccgg tgattttgtg
     7681 cagctttgca cgcacttcat caggcatgtc tgccgtggcg gtcagtgcaa gggtctccgt
     7741 cagcaggcgg gtatccacag actcaccggc agcgtgggcg cgcatggccg cgagcgccac
     7801 ctcctcggtg aacatatacg gcggggtgcg gcggtgttta cccggcatgg tcagaccgta
     7861 cttcagggca taacgggcaa tctccagcgc accggcaata tcgccggtat ccagacgcca
     7921 cagcatgacc gtcatcagaa tgtcatcctg tgcacctttg ccctgctcca gcacgccgtt
     7981 cacccacggc aaccagaacg gcagcagttc gcgttttttc gcggccttca gctcttttga
     8041 ataaatcgct ttcagtgtgc gctggtctgc ggccagcttg accagcatct gctcatagac
     8101 agttgcatgt cgcagcgggg cggcgtcccg ctgcgcggtc atcgctgccg agacccgcat
     8161 catgtggcgc tgtgcgggac tcgtcatcgg ttacgctccc ggctctgcgg tcactttagt
     8221 cggtgtggag aagtcaccga ccttaatttt ttccaccaga caaccggcgg cgtagtcttc
     8281 caccacgtaa tcaatgttca ttgactcgta gttctccacg cggtcgagtt tcgggttttc
     8341 cacaatcacg cggcgatggc tgtcatccat gtagtagatg gacaggtttt ccagcttcgt
     8401 gatgagcatc gcatccgccg ggaagtacgg gacgcgtacc gccggcaggt taccgatgcg
     8461 tttctggctg atgatgacgt cagcggccag catctcgctg ttgtcctgct ccctgttgac
     8521 gatgggaaaa tacttgtccg ccagcagctg acgccccaca atcaccacaa ggtcagggtc
     8581 ttcctgatac cacggctcaa tcaggttgtt ggtcgcatcc atcaccagtg catcgaggct
     8641 ggcataatca ccgcccttac ccacgcggat gacctcagag gtcgtgtgac cttcctcgtc
     8701 agtaaccttg ctcatcacgc gcgccggggc ttcattgcgg tatttctgca gccagccgac
     8761 cgccacatcc tgcagcatct ggttactgct gcggtcagag gtttcggcac gcctcacgcc
     8821 gttaaaaccg gccatgatta aatcaaggga ctggcgtttg ataatggcgt tacggacacg
     8881 gagctggaaa tcctgataac gcgcccacag gtcaagcgtt ttgtagcgga tataaaaatc
     8941 gaagttaatc tggtcgcatt cgtacttgtt tgacgccagc ttcgagaagt ccttcggctg
     9001 acgctcggtg ccaccggcgg tgtctgtggt gctggcgatg gagccggtga caccaatacc
     9061 aattttttcc cctttcattt cgctgaccgg cacaatgttg atgcgggtca gaaagtcaga
     9121 ggactcctgc atggtgttca tcagggtctg ggtgaccgac ggttcaacgg tgaatttttt
     9181 cgacacatca ccggcgtcga tgccgttcag ttcggcaaca cgggacaggt aggcattaaa
     9241 tttaaagcgg gtttcctggc gcatagtttt tcctgaaatt aagggttaat cgtgaaggtt
     9301 ttcccggact ggctgacgcc ggtcagcagt tcgtcatcag ggcgtcaccg ccaccgccgg
     9361 tggccttgct gcggcgctgc tgggtcagac tttcggtgtg gtcgagactg tttttcaggc
     9421 ggctgaatgc ctggctggtt tcatccgccc tgtcagtcac ctcctgctta agtgcggaaa
     9481 aggcggtttc catctcagcg agtcgctgct cagtggcgct cagcttttcc tgcacatgtt
     9541 cagcaacagc ggtcaccgct tcatgcacgt cattcagacg ggcgtcatcg ctggcctgtt
     9601 tgcggccaaa aatggatttc accttttcgg tcagggcggt gaacacggtt tcaggcaggt
     9661 cttcaaattc cagctcaaca ggcgttgcta ctgaaatcag gttttcaggg cttaatttga
     9721 agcggttcag ggggttgtgt tttgccgtgc ggcagaattc caggtattcc gtgccgaggc
     9781 ttgccgggtc atcggtgacg gccagaccca ccagataaca tttgccggtg ttggcaaagt
     9841 tcggctgaat ttccattgag gtgtagacct tctgcgcggc cttgttcatc gcgataaggt
     9901 catcggtcgg ggtgattttc gcaaacagcg cccatttgcc tttcagcgcc gaatcgtcat
     9961 caatcttttc ggccttcagt tcggccacat cgccataacg tttaaaaata ccgtcaggca
    10021 ggatgccgcg cagatgttcc aggttaatgc ggcaaccata gacacgcggg tcaaaggttt
    10081 cggccatttc ctgaatatcc tgcgcactga tgacacgccc gtcacaggtg tcaccctcaa
    10141 cgccgatacg aaagaatttt gagacttttt ttgccattgt caggagtcct gaatagtgat
    10201 tagaagagtc acatgtcggc atcagtttcc cgacgatgcg catcctccgc catcagtccc
    10261 ggatggctta tcactgacac aacagcacct tagcgaatcg cggggcgcga ctcagtagcc
    10321 ttgccgtgta ttcatcacgg cgaggtattc atgaccatca ccacagacac cactctttta
    10381 cacgacccgc gtcgtcaggc ggcgctgctg tactggcagg gattttccgt gccgcagatt
    10441 gccgccatgt tgcagatgaa acgcccgacg gtgcagagct ggaaacagcg cgacggctgg
    10501 gacagcgttg cccccatcag ccgtgtcgaa atgagtctgg aagcgcggct gacccagctc
    10561 atcatcaaac cgcagaaaac cggcggtgac ttcaaggaaa ttgacctgct cggacgccag
    10621 attgaacgac tggcacgggt aaaccgctac agccagaccg gcaacgaggc agaccttaat
    10681 ccgaacatcg ctaaccgcaa caaaggcggg cggcgcaaac cgaaaaagaa ttttttcagt
    10741 gacgaggcta tcgaaaagct ggagcagatt ttctttgagc agtctttcga atatcagttg
    10801 cactggtatc gcgccgggct tgagcaccgc atccgcgata tcctgaaatc ccgccagatt
    10861 ggcgcgacgt tttatttttc ccgcgaggcg ctgctgcgcg ccctgaaaac cggtcataac
    10921 cagatttttc tgtcggccag taaaacgcag gcgtatgtgt tccgcgaata catcatcgcc
    10981 tttgcccggc tggttgacgt tgacctgacc ggtgacccga ttgtcctggg caataacagc
    11041 gcaaaactga tttttctcgg caccaactcc aacaccgcgc agagccataa cggcgacctg
    11101 tacgtcgacg agattttctg gataccgaat tttcaggtac tgcgtaaggt ggcatcaggt
    11161 atggcctcac agagtcacct gcgctcgacc tatttctcca ccccgtccac gctggcgcac
    11221 gacgcctacc cgttctggtc gggtgaactg ttcaaccggg gacgcgccag cgccgccgaa
    11281 cgcgtggaaa tcgacgtcag tcataacgct cttgccggtg ggcttctctg tgcggacggt
    11341 cagtggcggc agattgtcac cattgaggac gccctgaaag gtggctgcac gctgttcgac
    11401 attgagcagc ttaaacgcga aaacagcgcc gacgatttta aaaacctgtt catgtgtgaa
    11461 tttgttgacg acaaggcgtc ggtgttcccg ttcgaggagc tgcaacgctg catggtcgac
    11521 acgctggaag aatgggaaga ctatgcgccg tttgccgcga atccgttcgg ctcccgcccg
    11581 gtctggattg gttacgaccc gtcacaccgt ggcgacagtg ccggatgcgt ggtgctggca
    11641 ccgccggtgg tggccggtgg caaattcaga atacttgagc gtcaccagtg gaaaggcatg
    11701 gactttgcca cccaggctga atccatccgc aaactcaccg aaaaatacaa cgtcgaatac
    11761 atcggaattg atgccaccgg cctcggtgtc ggcgtgttcc tgctcgttcg ctcgttctat
    11821 cccgccgcac gcgatatccg ctacacgccg gaaatgaaaa ccgcaatggt gctcaaggca
    11881 aaagacgtta ttcgccgtgg ctgtctggaa tatgacgtca gcgccaccga catcaccagc
    11941 tcgtttatgg ctatccgcaa gaccatgacc agcagcggac gcagcgccac ctatgaggcc
    12001 agccgcagcg aggaagccag ccacgccgac ctcgcctggg cgaccatgca cgccctgtta
    12061 aatgagccac tcaccgccgg tatcagcacc ccgctgacat ccaccattct ggagttttac
    12121 tgatgagcaa gaaaaaaggg aaaacaccgc aacctgcggc aaaaaaaatg accgccagcg
    12181 ccccgaaaat ggaggcattc acctttggtg aaccggtgcc ggtactcgac cgccgtgaca
    12241 ttctggatta cgtcgagtgc atcagtaacg gcagatggta tgagccaccg gtcagcttta
    12301 ccggtctggc aaaaagcctg cgtgctgccg tacatcacag ctcaccgatt tacgtcaaac
    12361 gtaatattct ggcttcaacg tttattccgc acccgtggct ttcccagcag gatttcagcc
    12421 gctttgtgct ggattttctg gtgttcggta atgcgtttct ggaaaagcgt tacagcacca
    12481 ccggtaaggt catcagactg gaaacctcac cggcaaaata tacccgccgt ggcgtggagg
    12541 aggatgttta ctggtgggtg ccgtccttca acgagccgac agccttcgcg cccggctccg
    12601 tgtttcacct gctggagccg gatattaatc aggagctgta cggcctgccg gaatatctca
    12661 gcgcccttaa ttctgcctgg ctgaatgagt cggccacgct gttccgccgc aagtattacg
    12721 aaaacggcgc tcatgccgga tatatcatgt acgtcaccga tgccgtgcag gatcgcaacg
    12781 atatcgaaat gcttcgcgaa aacatggtca agtcgaaagg ccgcaacaac tttaaaaatc
    12841 tgtttctcta tgccccgcag gggaaagccg acggcattaa aattatcccg ctcagtgaag
    12901 tggcaacgaa ggacgatttt tttaatatca aaaaagccag cgccgctgac ctgctggacg
    12961 cgcaccgcat cccctttcag ttgatgggcg gcaagccgga gaacgtcggg tcgctgggtg
    13021 atattgagaa agtggcaaag gtctttgtcc gcaatgagct tatcccgcta caggacagga
    13081 tccgcgagat aaacggctgg ctcggtcagg aggtcatccg ctttaaaaac tactcactgg
    13141 acactgacaa cggctgaaca tcgccgcctg cgggcggctt ttttacaccc cgtcatcacg
    13201 ccctcacacg ctcaccaccg cacaaaacac cccgcagaca caccaacgcc tcaacgggca
    13261 gactaagcgc catcacgacg cgctcagacg ctgaaaaaat aaaatcagca ccaccgccag
    13321 cgcgcagtgc tttccccgcc tcgcccgccc gcttcatgtg gcgattttaa tgcggttgca
    13381 tttttatcga aagtctcgtc tcacaaatcc ctcccttcac taaatagact cattatccac
    13441 atgcaatcta attcaa
//
LOCUS       DAZKIP010000116        13412 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZKIP010000116 DAZKIP010000000
VERSION     DAZKIP010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.117"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..443
                     /locus_tag="ACHOUK_003708"
     CDS             <1..443
                     /locus_tag="ACHOUK_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HFP9899075.1"
                     /translation="SNILFDYAWFDNTIFIKTLFKNTCFYNVRAKNVYLEGAYLNNDN
                     IVNQANNSTEKQSIDSTDKQANDSTVQQSIDSTVQQANDSTDKQANDNIDKQVNDSTD
                     KQAKNSTEQQDSNSFNQARLKKEVNRRFSIPGLTSYQPTYIVEE"
     gene            709..1551
                     /locus_tag="ACHOUK_003709"
     CDS             709..1551
                     /locus_tag="ACHOUK_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313321.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFP9899076.1"
                     /translation="MKKRITFIVFLCAIVAASLFFVQSCVRKSQHVAGFQNYQATIDG
                     KEITGVTKNISSLTWSAQSNTLFSTINKPATIVEMTTEGDLIRTIPLDFVKDLETIEY
                     IGDNKFVISDERDYAIYVISLNADSEVSILKKIKIPLQETPTNCGFEGLAYSSQDHTF
                     WFFKEKNPIEVYKVTGLLRSDELHISKDKTLQRQFTLDDVSGAEFNPQKNTLLVLSHE
                     SRALQEVTVRGDVIGEMSLTKGKYGLSHNIKQAEGIAMDDSGNIYIVGEPNLFYRFTS
                     TKSR"
     gene            1619..2797
                     /gene="yjiJ"
                     /locus_tag="ACHOUK_003710"
     CDS             1619..2797
                     /gene="yjiJ"
                     /locus_tag="ACHOUK_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9899077.1"
                     /translation="MNSTSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMLAE
                     GEFSFSELSWIASGNYAGYLAGSLLFSFGAFHLPSRLRPFLLASALATGLLILAMAWL
                     PPFLLVFIIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGVGIALGNEYVLA
                     GLHFALSSQTLWQGAGALSAIILLALALLIPSNKHVIPPAPLAKIAQQPMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGQPVLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAICVLLTLASSSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALASATLCGAAALFIAALICGMQIFKLHTNYS
                     "
     gene            3008..8249
                     /gene="iatD"
                     /locus_tag="ACHOUK_003711"
                     /pseudo
     CDS             3008..8249
                     /gene="iatD"
                     /locus_tag="ACHOUK_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168882.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter IatD"
     gene            complement(8406..9001)
                     /gene="kptA"
                     /locus_tag="ACHOUK_003712"
                     /pseudo
     CDS             complement(8406..9001)
                     /gene="kptA"
                     /locus_tag="ACHOUK_003712"
                     /inference="COORDINATES: protein motif:HMM:NF014001.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
     gene            9206..9889
                     /gene="yjiH"
                     /locus_tag="ACHOUK_003713"
     CDS             9206..9889
                     /gene="yjiH"
                     /locus_tag="ACHOUK_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFP9899078.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            9886..10347
                     /gene="yjiG"
                     /locus_tag="ACHOUK_003714"
     CDS             9886..10347
                     /gene="yjiG"
                     /locus_tag="ACHOUK_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFP9899079.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            10360..11532
                     /gene="iadA"
                     /locus_tag="ACHOUK_003715"
     CDS             10360..11532
                     /gene="iadA"
                     /locus_tag="ACHOUK_003715"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFP9899080.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIVRAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            11654..12508
                     /gene="hypT"
                     /locus_tag="ACHOUK_003716"
                     /gene_synonym="qseD"
     CDS             11654..12508
                     /gene="hypT"
                     /locus_tag="ACHOUK_003716"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFP9899081.1"
                     /translation="MTLEKCRNFSQAAVSRNVSQPAFSRRIRALEQAIGVELFNRQVT
                     PLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRKIKIAAAHSLSLGLLPSIIS
                     QMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLLEAPFDHIRLFESQLFPVCA
                     SDEHGEALFDLVQPHFPLLNYSRNSYMGRLINRTLTRHSELSFSTFFVSSMSELLKQV
                     ALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAYAYRMNTRMNPVAERFWREL
                     RELEIVLS"
     gene            complement(12501..12893)
                     /gene="iraD"
                     /locus_tag="ACHOUK_003717"
     CDS             complement(12501..12893)
                     /gene="iraD"
                     /locus_tag="ACHOUK_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFP9899082.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSTLPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(12890..12973)
                     /gene="idlP"
                     /locus_tag="ACHOUK_003718"
     CDS             complement(12890..12973)
                     /gene="idlP"
                     /locus_tag="ACHOUK_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFP9899083.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(13089..13232)
                     /gene="ytiD"
                     /locus_tag="ACHOUK_003719"
     CDS             complement(13089..13232)
                     /gene="ytiD"
                     /locus_tag="ACHOUK_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtiD"
                     /protein_id="HFP9899084.1"
                     /translation="MADYAEINNFPPELSSSGDKYFHLRNYSEYSEYTSGFFLSLMIF
                     IKS"
BASE COUNT         3544 a         3322 c         3215 g         3331 t
ORIGIN      
        1 gttcgaatat cctatttgac tacgcatggt ttgacaatac aatatttata aaaacgcttt
       61 ttaaaaatac ctgtttttac aatgtcagag cgaaaaatgt ctatcttgag ggagcatatc
      121 tgaacaatga taatatcgtg aatcaagcca ataacagtac cgagaaacaa tccattgaca
      181 gtaccgataa acaggccaat gacagtacgg tgcaacaatc cattgacagt acggtgcaac
      241 aagccaatga cagtaccgat aaacaagcca atgacaatat cgataaacag gtcaatgaca
      301 gtaccgataa acaagccaag aacagtaccg agcaacagga cagtaacagt tttaatcaag
      361 cccgtttaaa gaaagaagtg aataggagat tttccattcc gggtttaacg tcttatcagc
      421 caacatatat agttgaagaa tagttcatta atcacccaaa cagagtgggt atggcagacg
      481 atatagatta acatttaaac gttaaccgcc tcatctatca ttcatagatg aggcgcattt
      541 atcagtcgat gtaagctgaa aatgttctgc catccagaga caaccgccgc cccctgcggc
      601 gtttactatt tcatcgcccg cgttaagaat tcattaagct tcatccgtat actccttatc
      661 tgtcgcactg aatttgacga gaaaaaagca ataataagga gcaataagtt gaaaaagcgc
      721 atcaccttta tcgtttttct ttgcgccatc gtggcggcaa gcctgttttt tgtgcaatcc
      781 tgcgtccgta aaagccagca tgtcgcaggg tttcagaatt atcaggccac tatcgatggt
      841 aaagagatta caggagtaac taaaaatatc tcctcactga cctggtcagc gcagagcaat
      901 accttattta gcactatcaa caaacctgca accatcgttg aaatgacgac cgagggcgac
      961 ttgatccgca ccattccgct cgattttgtt aaggatctcg agacgattga atacattggt
     1021 gataataaat ttgtcatcag tgatgaacgt gactacgcca tttatgtgat ttcacttaac
     1081 gctgactcgg aagtgagcat cctcaaaaaa attaaaatcc cattgcagga aacaccaact
     1141 aactgtggct ttgaagggct ggcttactcc tctcaggacc ataccttctg gttttttaaa
     1201 gagaaaaatc cgatagaggt ctacaaagta accgggctat tgcgtagcga tgagttgcat
     1261 atcagcaaag ataaaacgtt gcagcgtcag tttactttag acgatgtttc tggcgcggaa
     1321 tttaacccac agaaaaatac gttgctggtg ttgtcacatg agtcacgcgc cctgcaggaa
     1381 gtgaccgtga gaggtgacgt gatcggcgaa atgtcgttaa ccaaaggtaa gtatggcctt
     1441 tcacacaaca tcaagcaggc ggaagggata gcgatggatg atagcggcaa catctatatc
     1501 gttggcgaac cgaatctttt ttatcgcttt acgtcgacaa aatcacgata acagtctggc
     1561 aggttctcct tttttgttat taacttgtta actcaaaata atacaaacca ggcagaacat
     1621 gaattcgaca tcgcatcccg tagaacgttt ttctttcagc accgcgttat tcgggatgct
     1681 ggttctgacc ttaggtatgg gtttaggccg ctttctctat acgccgatgc tgccagtcat
     1741 gctggcggaa ggcgagtttt catttagcga actctcatgg atcgccagtg gtaactatgc
     1801 cgggtatctg gcggggagcc tgctgttttc attcggcgcg tttcatttac cctcacgcct
     1861 gcgcccgttc ctgttagctt ccgccctcgc aaccggatta ttaatcctcg cgatggcgtg
     1921 gctgccgccg tttcttctgg ttttcatcat tcgctttctg gcgggagtcg ccagcgccgg
     1981 gatgttgatt ttcggctcaa cactcatcat gcaacatact cgccatccct ttgtccttgc
     2041 ggcgctattt tctggtgttg gcgtcggcat cgctctgggt aatgaatatg tgctggcagg
     2101 cctgcatttt gccctctctt cacaaacgtt gtggcaaggt gccggagcac tttctgccat
     2161 tatattgctt gctctggcgc tgctcatccc gtcgaataaa cacgttatcc cgccagcgcc
     2221 attggcaaaa atcgcgcaac aacccatgag ctggtggtta ctggcgattc tgtatggtct
     2281 ggcgggtttt ggttatatca tcgtcgccac ctacctgccg ctcatggcga aagacgcggg
     2341 ccagcctgtg ttgacggctc acctctggac actggttggc ttgtcgattg tcccaggttg
     2401 ctttggctgg ctgtgggcag ccaaacggtg gggagcatta ccttgcctga ccgcgaattt
     2461 gctggtgcag gcgatctgcg tgctgttaac cctcgccagc agctctcctt tattactcat
     2521 catcagcagt attggttttg gcggcacctt tatgggaacg acctcgctgg tgatgaccat
     2581 cgcccgccag cttagcgtgc cgggaaatct taaccttttg ggctttgtga cactcattta
     2641 tggtatcggg caaattcttg gcccggcgct gaccagtatg ctcggcaacg gaacgtcggc
     2701 gctcgccagc gccacactct gcggcgcagc ggcgctattt atcgcagcat taatctgcgg
     2761 gatgcaaata ttcaaattgc atacgaatta ttcttaagta atttaactaa tttaactttt
     2821 cagcacatca cgtaaaataa ttaaaatatc ctttttcttt ggaggtactc agccaccgcc
     2881 ggtattattc ctggtttagc aattccacgg ctaattcatt atttctggtc atattatagt
     2941 caggatcgca ttatatctcc gtgctagaaa aaacaagttt aattctgttt tttcacggaa
     3001 ggtcactatg gcttcgacaa atgcgcatca aataaaaaat aatgatcaaa actctttatg
     3061 tggtctgggt gacaaaatac gccgcctgac cgccggagtt tgtttattta cacaattttt
     3121 tttccccgtc atggcgacgg cacaaaatgt ggtacatgct aaacctcaga cgacagtttc
     3181 atctgccccc ccctctacta gaaaataata ctgtgcctta cacgcttggt gcgctggaat
     3241 cagcgcaaag cgttgctaat cgcttcggta tttcactgga ggagcttcgt cgtcttaatc
     3301 agttccgtac ttttgctcgc ggctttgata acgtgcgcca gggtgaagaa ctggatgttc
     3361 cggcaacaac ctcgcagaaa agccatgagc aacaaaatgc cgtaccgcct gcgaatggcg
     3421 aaaacacgct ggagaatcaa atagccagca cctcgcagcg agttggcact ctgctttcac
     3481 aagatatgaa tagtgagcag gccagcggca tggcgcgtgg ttgggcgtct tcagaagcct
     3541 caggcgcgat gactgattgg ttaaataact ttggtactgc gagaatctct ctgggtgtgg
     3601 atgaagattt tagcctgaaa aattcgcaat tcgacttcct gcatccgtgg tatgacacac
     3661 ctgattatct gctcttcagc cagcataccc ttcaccgaac agacgatcgt acccagatca
     3721 acaccggttt gggctggcgt catttcacct ccagctggat gtcaggcatc aacctttttt
     3781 ttgaccacga cctgagccgc tatcactccc gcgcagggct tggcgcagaa tactggcgtg
     3841 attatctgaa gttgagcagc aacgcttata tcggcctgac cggctggcgt agcgcaccag
     3901 aattggataa cgacttcgaa gcccgcccgg ccaacggctg ggatttacgc gcggaaggct
     3961 ggttacctgc ctggccacaa ctggggggaa aactggtcta tgaacaatac tatggcgatg
     4021 aagtggcgct gtttgacaag aatgatcgtc aaagtaaccc ccatgctatt accgcaggcc
     4081 tcaactatac ccccttcccg cttctgactc tcagtgcgga acagcgtcag gggaagcaag
     4141 gtgaaaatga cacacgtttt gccgttgatc tgacctggca acccagcagt tcaatgcaga
     4201 aacagcttaa tccggacgaa gtggccggac ggcgcagtct ggccggtagt cgttatgacc
     4261 tgattgatcg caacaacaac atcgttctgg aataccgcaa gaaagagctg attcgcctga
     4321 gtctgctgga tccggtgaaa gggaagtctg gagaaataaa accgctggtt tcctcgctac
     4381 agaccaaata tgcccttaaa ggctataaca tcgaagccgc tgcgctggaa gctgccggag
     4441 gtaaagtcag cacgtctgga aaagatatca cggtcacgct gccaggttac cgcttcacta
     4501 acaccccaga aaccgataat acatggtcga tagacgttac cgccgaggat gtaaaaggta
     4561 acctgtcacg gcatgaacaa agcatggtag ttattcaggc tccgacatta agccagaaag
     4621 attctctgtt atccgtcaat ccgctaaccg tggctgcaga taaaaaatcg acgaccacat
     4681 tgaccgttac tgcgcacgat tccgacggaa ctccggtgcc ggggctggcg ctgcaaaccc
     4741 gcagtgaagg cgttcaggat atcaccctgt ctgactggac agataacggt gatggtagtt
     4801 acacacagat actgaccgcc ggaacgacat caggttcagt aacactgacg ccgcaaatta
     4861 acggtgagag tgcggtaaaa gaatccatcg tcgttaatat cgtccctgtt gtctcatccc
     4921 gcgaccattc atcaataaca attgataacg tatcgtatta tgccggagac gacatcaagg
     4981 ttagggtgga actgaaagac gatagcaatc aaccggttgc atatcaaaaa gaggaattgg
     5041 taaaagccgt tactgtcgaa aacagcaaac ctggcgccac gattgtctgg cacgaagagc
     5101 agccgggggt ttatgccgcg aattatccgg cctataagca agggactgca ctaagggcac
     5161 aacttagcct tcacaactgg aatgctccac tgcaatcgca tatttataac attgaggcaa
     5221 accagaataa ggctcgcgtt gccacattat cagcgacaaa taatgacgtt tacgccgata
     5281 aaaagacatt taataccctc acgatcaacg tcactgatga gagtgataat cccctgacaa
     5341 atcatcaggt cacctttaag aatgaaaaag gaagcgcgga gtttgtcgaa ccgccgcagc
     5401 aaaatacgga tgcatatggt gttgccacaa taaacatggt aagtcaggtt gcggaagaaa
     5461 atacgattag cgccacgctg ccaaatggtt tttcacaacg gataattgcg aaattcgtta
     5521 gcgattcgag tacgccaaaa ttcaaacaac tggttgccga tccagatacc attattgctg
     5581 gcaacagcca gggcagtact ctgaccgcca tcatcacaga ctttcataac aacccgttaa
     5641 aagatatgaa agtgaatttt gtggcacctg gtggctcgca actggacaac acgaccgcca
     5701 caacagacca gtccggtatt gtgcgggtgc acctgaccag ttcaaaagct ggtagctatt
     5761 ccgtcgatgc ctcgcttgag gtggataaaa atattcacca gtcggtcacg atcaccgtgg
     5821 tcccaaacag ggaacaatcg gtaatgacct tgaatgccgg gtcgggcagt gcgatcgcta
     5881 acaatacaaa tatcgttacc ctgactgcca gtgtgaaaga tgtttatgga cacccgttgc
     5941 cggatgagga tgtgaaattt accttgccag cctccatgac cgggaacttc acgctaagta
     6001 gtgaaaccgc ccgcaccgat gcaaacggtg atgccgtggt cacattgcga ggcacaaaag
     6061 cgggtgagtt tacagttacg gcgacgctga ccagaaataa taccgttgct tatcagcaag
     6121 tcacttttat tggggataca aacagtgcgc agctccagcc gctgactgcc tcattaaatt
     6181 ccattgttgc gggtaacagt acggggagta ccctgacggc aacgatcctg gacgcttacc
     6241 aaaatccgct taaagaccag ttggtcactt tccagagtaa cgatgtcact ctaagcgaaa
     6301 cagaagtcac caccaatacg ctgggtcagg cgacggtaac aatgaccagc aatattgccg
     6361 gacaacataa cgtcgtggtg agccggaaag cgcaagcttc cgataataaa acgtttagtt
     6421 tatcagtgct accggatgaa agttcggcga aggtaataag tataaccgga gccgaaaaaa
     6481 cgataacggt gggcgaaaac atcacgctac ggatactcgt ccaggacgcg tttaacaatg
     6541 taatcgcggg tcaacgcgtc agattaagtg cgcagccaac aactaacatt acgataggcg
     6601 atacggctta caccgataat aacggttatg cgtacgttaa ccttctcagc acccaacctg
     6661 gggtttatca ggtgacggca acgctggaca ataacagtag tagtaaggtt gacgtgaatg
     6721 tggcaaatgg caaactcgag ttaacatcat cgaaaccaga aactacggtc cataatagtg
     6781 agggtattac gctgaccgca acggcgagaa atgcgcgggg tgaattgatg ccagggcaaa
     6841 ttatcacctt tagcgtaacg cctgaaggtg caacgctaag caatacaggg gaagtcctta
     6901 ctgaccagtc aggtcaggcc aaagtgacgc tgaccagtga caaagtgaat gtctataccg
     6961 ttacggccat aatgggcaaa gatgttcccg ttcagagcca ggtaacggtt gcggttaagg
     7021 cagatgctaa aacggcacat gttgtgagcg tcgtggcttc tcctgacacc atcaccgccg
     7081 acggcatcga tagcagcacc atcacttcac gagtagaaga tgattacgga ttcccggttg
     7141 aaggtgtcga tattagtcat ggcttagaca ccaaaggcag cccggtagtt aatattccaa
     7201 ctacgcgtac cgatcagtcc gggcaagtca cggcgacaat aaccagtaca ttggcagaaa
     7261 ccttaacagt caatgtgcaa gttcctggca cagccaacca atccgcaacc attacattgg
     7321 ttgccggcac ggccgatgaa agtaagtcaa ttttgaaatc cgatgttgac actctgaagg
     7381 ctgactacca gcagagcgca aaacttacgc taacattgca agacaagtac ggtaacccga
     7441 tagtgacgtc tgatcatctg gaatttgtcc agtcaggccc cttcgtgaac tttctcaagt
     7501 tgagcgatat tgattacagc caaagaaatt atggcgagta caccgtgact gtcactggcg
     7561 gaaaagaggg aacagcgaca ctcattccca tgctgaacgg ggttcatcag gcaaacttaa
     7621 gcatatcgct gaatctcatc caatcgataa aagaaatgtc cggtcatgtc actgcaaaca
     7681 accatacctt ctccacggct aaattcccga gcgaaggctt tgcaggagcg tattacacac
     7741 tcaacaatga taactttgaa gcgggtaaaa ccgttgatga ttatatgttt tcaagttcac
     7801 agggttgggt gtctgtcgat gcttcgggta aagtttcttt cgcaaatatc ggcgatcaaa
     7861 cgtcagtcac aataagcgct gttccccgac aaggaggtac aacctaccag accttaatta
     7921 agctgaaagg ctggtgggtg aataatggaa atcataccaa tatctggcta gctgccaatg
     7981 cgctctgtca tgctaaaaat gatggatata atcttcctgg catcacacat ttgacgtctg
     8041 gcgaaaacaa acgcacgcag ggatcactgt atggtgaatg ggggaacgtt ggagcgtttt
     8101 ccagtaattc gcaatttaca ccgggagctt actggacaag tgaatctgat gattacagtc
     8161 ggcactacta tgtgcagatg ctaaccggta tgaccggaag cgacgctgat tccagccccc
     8221 aactgaccgc ctgccgtaaa tcactttaat ttcagcagtt gttattaaag gatacccttt
     8281 tacgagggta tcctttcaga ctgataacaa acgctacatt gccggatgtg ctgcgcttat
     8341 caggcctata taacccctgc aatatattga atttattgtt tttgtacccc agccggcata
     8401 aacgactact caagaaactc aacagcaacc gttgatgtca gccagacccc gttttccgct
     8461 tgccagaaag ggatgcctcg tttcgccatt tcctgtgctt tgacggttaa gacaaccggc
     8521 gatccgtgtc ttgctcccac tttgcgtgcc gttgcttcat cagcagagag atgcacgtaa
     8581 tgacgatccc ctgcaatcag cccctgtttt tttatctcgt caaggaaacg gcttgccgta
     8641 ccgtggtaga gaaactgtgg cggtgttttt tcggcaaagg aaatagcgac ctgcgaagtc
     8701 gaatgcccct gaaccgcgcg gatgcaccta ccatcactgg aataactaaa acgcttttat
     8761 cgctggttgc gaccacggta tccagcaatg cacgggttaa tcgcttgccc gctttttggg
     8821 cacaaagtat gagcttatcg atattcgccc atccttcacg gtccagtacg ataccaatcg
     8881 cctccggttt gtgtcggagg acaaaactta aaaacttgct cgtatccgcc agctcttttt
     8941 cgttgtactt tgccatcgcg ttttcgtccc tggattttct gtttttattt accaatgtca
     9001 ttgtcactgg gtttatgaat tttctgcaac tcaatcctgc gcagaacctt ttccacacag
     9061 cgtaattttt cccctatgca aaaaacgcat agccagaaac atacttgcat tttccccata
     9121 cccacatggc tgataaggtt tttaagttcc aggaattttc ctggctcaag caacacacaa
     9181 acacaaaaaa acaatgggga ttgttatgac acaacaaggg gatgctgtag ccggggagct
     9241 ggcgacagaa aaagtgggca ttaaagggta tctggctttt tttctcacca taatcttttt
     9301 ttccggtgtt ttttccggaa cagacagctg gtggcgcgtt tttgattttt ccgttctgaa
     9361 cggatcgttt gggcaactgc ctggtgcgaa tggagccacc acctcatttc gcggcgcggg
     9421 tggcgcgggg gctaaagatg gctttctctt tgcgctggag ctggcaccgt cagtcattct
     9481 ctctctgggg attatttcaa ttaccgatgg tcttggtgga ttacgcgccg cgcagcagtt
     9541 aatgacacct gtgctaaaac cgttgttggg cattccaggg atctgctcgc tggcgctgat
     9601 cgctaacttg caaaacactg atgccgccgc cggtatgacc aaagagctgg cgcaggaagg
     9661 tgaaattacc gaacgcgata aagtgatttt cgccgcatat caaaccagcg gtagcgccat
     9721 catcaccaac tacttctctt ccggcgtcgc ggtgttcgcc tttctgggca cgtccgtaat
     9781 cgtaccttta gccgtcatcc tggtgtttaa atttgtcgga gccaacattt tgcgcgtctg
     9841 gcttaacttc gaagaacgtc gcaacccaac gcaaggagcg caagcatgac aactcaggtc
     9901 cgcaaaaacg tcatggatat gtttatcgat ggcgcgcgcc gtggctttac tatcgccacg
     9961 accaacctgc tgccaaacgt ggtgatggcg tttgtcatca ttcaggcgct gaaaattacc
    10021 ggcctgctcg actgggtggg gcatatctgt gaacccgtaa tggcgctgtg gggattaccc
    10081 ggtgaagcgg caaccgtcct tttagccgca ttaatgagta tgggcggcgc agtcggtgtt
    10141 gccgccagtc tggcaaccgc tggcgcatta accggtcacg atgtcaccgt tttactgccc
    10201 gcgatgtatt taatgggcaa cccggtgcaa aatgtcggtc gctgcctggg gacagccgaa
    10261 gtgaatgcca aatattaccc gcatatcatt acggtctgcg tgattaacgc attgctgtcg
    10321 atctgggtta tgcagcttat tgtttaacaa ggagttacca tgattgatta taccgcagcc
    10381 ggttttaccc tgctgcaggg agcgcatttg tatgcgccgg aagatcgggg aatttgcgat
    10441 gtcctcgtcg ctaacggcaa aattatcgcc gttgccagta atatcccttc tgacattgta
    10501 ccggactgca cggttgtcga tctcagcggg cagatcctct gcccaggttt tattgatcaa
    10561 cacgtccatt tgattggcgg tggcggcgaa gcaggtccca cgacgcgcac gccggaagtg
    10621 gcgctaagtc gcctgacgga agcgggcgtc acgtcagtgg ttggtctgct gggcaccgac
    10681 tctatctctc gccacccgga atccctgctc gccaagaccc gtgcgctcaa tgaagaaggc
    10741 atcagcgcct ggatgctgac cggcgcttat catgtccctt cccgcaccat tacgggttcc
    10801 gtggaaaaag acgtggcgat tatcgatcgt gtgattggcg tgaaatgtgc catctctgat
    10861 caccgttctg ccgcaccgga cgtttatcac ctggctaata tggcggcaga atcccgcgtt
    10921 ggcggtttgc tcggcggtaa acctggcgtc accgtgttcc acatgggcga cagtaaaaag
    10981 gcattacagc ctatttatga cctgctggaa aactgcgatg tgccgatcag caagctgctg
    11041 ccgacccacg ttaaccgcaa cgtaccgttg tttgagcagg cgctggagtt cgcgcgcaaa
    11101 ggcggcacca tcgatatcac cagcagcatt gacgaaccgg tcgcccctgc cgaaggtatt
    11161 gtccgcgccg ttcaggcggg tattccgctg gcacgcgtca ccctcagctc cgacggcaac
    11221 ggtagccagc cgttcttcga tgatgaaggg aatttaaccc atatcggcgt tgcaggtttt
    11281 gaaacgttgc tggaaaccgt gcaggtgctg gttaaagact atgatttcag tatcagcgat
    11341 gccctgcgcc cgctcaccag tagcgtagcc ggtttcctta acctgaccgg gaaaggcgaa
    11401 attctgccag gcaatgacgc agacttactg gtcatgacgc cagaattgcg cattgagcag
    11461 gtatacgctc gcggcaaact gatggtcaaa gacggcaaag cctgcgtgaa aggaacgttt
    11521 gaaacggctt aaacggttgt cagccgccct aatggacgta gaatgccccc aagggcggct
    11581 gacagagtaa aacgtaatgg atgactgtgg tgcggttttg cataatattg aaaccaaatg
    11641 gctttaagat tttctgaccc tggaaaaatg ccgcaatttt tcccaggcgg cagtcagtcg
    11701 taacgtctcg caaccggcat tcagccgccg catccgcgcg ctggaacagg cgattggcgt
    11761 tgaattgttt aaccgccagg tgacgccgct gcaactctca gaacaaggga aaatcttcca
    11821 ttcgcagatc cgccatctgt tgcaacagtt agaaagcaac cttgcagagc tgcgtggcgg
    11881 cagcgattac gcgcaacgta aaatcaagat agccgctgca cactctcttt ccctcggtct
    11941 gttaccgtcc attatcagcc agatgccgcc gctctttacc tgggcgattg aagccattga
    12001 tgtcgatgaa gcggtcgata aactgcgtga aggacaaagt gactgtattt tttcctttca
    12061 cgacgaagat ttgctggaag cgccgtttga ccacattcgc ttatttgaat ctcaattgtt
    12121 ccctgtctgc gccagtgacg aacatggaga agcacttttt gacctcgtgc agccacactt
    12181 tccgttactg aattacagcc gcaactccta catggggcga ttgattaatc gcaccctgac
    12241 gcgccacagt gagttaagtt tcagcacctt ttttgtctct tcgatgagcg agcttttaaa
    12301 gcaggttgcc ctcgacggct gtgggattgc ctggctgccg gagtatgcca tccaacaaga
    12361 aattcgcagc gggcagctcg ttgtgctgaa tcgggacgaa ctggtgatcc cgattcaggc
    12421 ctacgcatac cggatgaata cccgcatgaa tccagttgcc gaacgcttct ggcgtgaact
    12481 gcgcgagctg gagattgtgc ttagctgaca ttctcaagcg tcgcactgcg ccaaccgcca
    12541 ttatcccaga ccagatcaaa cgttagcggt tcatcgtcgt ggtacagcat tatctcaagg
    12601 ctgatcgcca gagtgtggca gtacgcttct ttcaaattaa cctgcaacgc cgcaatccgt
    12661 ggctcatgaa ataacagtgc agaacgcagc tgacggcaat accagctctg gagatgtttg
    12721 tcgctcgggt tatgcacttg ccactcgggc atgcctgaaa caagtaatga cggtaatgtc
    12781 gaatgaggag aattaaaaag agtgaggaga tcgctgcata ccgatttacg cagcaatgac
    12841 gttttattgc ctgaaatttc aggtaaaaca gcctgaagtg attggcgcat cattttgcgc
    12901 actcctgacg tttagcaaca tatgcggctt gccctgaaca ccgggcacca ctgtattgat
    12961 gctcattttc catagccatt cccttttgtt cattacatcc atgtcagtct gagacaggcc
    13021 tgaatcctgt cagcaaagta taaaacctta cttgcctatg aatatctact gacttttcag
    13081 gaaaaaagtc atgatttgat aaaaatcatc aaactcaaaa aaaaaccgct agtatattct
    13141 gaatattccg aatagttacg tagatgaaaa tacttgtcac cgctgctact taattccggt
    13201 gggaaattat ttatttccgc ataatcagcc atgtattatt tgaaacaatt agtttgtaaa
    13261 taatataaat tgataacata tcaaatacat caaaaacacc attcacaggc attattaata
    13321 tagacctcaa tatagcaaca tcaaattcca attagtaata agatatgatt ttaaattgca
    13381 tgcaaataat tttaaatgag cgttttttga ta
//
LOCUS       DAZKIP010000117        13402 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZKIP010000117 DAZKIP010000000
VERSION     DAZKIP010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.118"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..1386
                     /locus_tag="ACHOUK_003720"
     CDS             <1..1386
                     /locus_tag="ACHOUK_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI
                     domain-containing protein"
                     /protein_id="HFP9899085.1"
                     /translation="RIAGISRDDGLITLQDAEGNTRLISPREAVAEGVTLYPPDTIRV
                     GTGDRMRFTKSDRGRGYVANSVWTVTAVSGDSVTLSGGQQTRVIRPAQERAEQHIDLA
                     YAITAHGAQGASETFAIALEGTEGSRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTD
                     AINNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPA
                     RFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGEGRVKGSGDAQF
                     VALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAITGGRVWGDIP
                     DNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLPDGRTEQA
                     VREIAGQERERAVTSEREAALPESVLREPQREREAVREVVRENLLQERLQQMERDMVR
                     DLQKEKTLGGD"
     gene            1406..2152
                     /gene="traX"
                     /locus_tag="ACHOUK_003721"
     CDS             1406..2152
                     /gene="traX"
                     /locus_tag="ACHOUK_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFP9899086.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSLLVWSPGQRDIIKTVALVLM
                     VLDHANRILHLDQTWMFLAGRGAFPLFALVWGLNLSRHAHIRQSAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSHRLYRAEDRTERLVLVACLLAVIPALNLASSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPTFYGCHLAVLGILAL"
     gene            2211..3071
                     /locus_tag="ACHOUK_003722"
     CDS             2211..3071
                     /locus_tag="ACHOUK_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9899087.1"
                     /translation="MKITDHKLSEGIALTFRVPEGNIKHPLIILCHGFCGIRNVLLPC
                     FANAFTEAGFATITFDYRGFGESDGERGRLVPAMQTEDIISVINWAEKQECIDNQRIG
                     LWGTSLGGGHVFSAAAQDQRVKCIVSQLAFADGDVLVTGEMNESERASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDDESKVFFEKIKARHPEMDIKIPFLTVMETLQYKPAESAA
                     SVQCPVLVVIAGQDSVNPPEQGRALYDAVASGTKELYEEADACHYDIYEGAFFERVVA
                     VQTQWFKQYL"
     gene            3174..3734
                     /locus_tag="ACHOUK_003723"
     CDS             3174..3734
                     /locus_tag="ACHOUK_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFP9899088.1"
                     /translation="MAEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTQDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            3867..4079
                     /locus_tag="ACHOUK_003724"
     CDS             3867..4079
                     /locus_tag="ACHOUK_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFP9899089.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGSRAVILEVSGRWMEAAEAWRRAAG
                     VAPRTDWQQFARKRAEHCHRRCRGRG"
     gene            4324..4785
                     /locus_tag="ACHOUK_003725"
     CDS             4324..4785
                     /locus_tag="ACHOUK_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFP9899090.1"
                     /translation="MRKYIPLVLFIFSWPVLCADIHGRVVRVLDGDTIEVMDSRKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRMLGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            4831..5040
                     /gene="hha"
                     /locus_tag="ACHOUK_003726"
     CDS             4831..5040
                     /gene="hha"
                     /locus_tag="ACHOUK_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFP9899091.1"
                     /translation="MEKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSTSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            5078..5416
                     /locus_tag="ACHOUK_003727"
     CDS             5078..5416
                     /locus_tag="ACHOUK_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899092.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSGGEYDASHLRPERRRRDILLEEVLRQAGIPLLRSHDARKLLQMTGEWLNTTG
                     AAQQSPEHRS"
     gene            5656..5910
                     /locus_tag="ACHOUK_003728"
     CDS             5656..5910
                     /locus_tag="ACHOUK_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFP9899093.1"
                     /translation="MSQTENAVTSSSGAKRAYRKGNPLSDAEKQRLSVARKRASFKEV
                     KVFLEPKYKAMLMQMCHEDGLTQAEVLTALIKSEAQKRCV"
     gene            6146..6220
                     /gene="tap"
                     /locus_tag="ACHOUK_003729"
     CDS             6146..6220
                     /gene="tap"
                     /locus_tag="ACHOUK_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFP9899094.1"
                     /translation="MLGKVQDFFLCSLLLRIVSAGWCD"
     gene            6213..7070
                     /gene="repA"
                     /locus_tag="ACHOUK_003730"
     CDS             6213..7070
                     /gene="repA"
                     /locus_tag="ACHOUK_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFP9899095.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSKGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTPALFAALDV
                     SEDAVAAARRSRVEWENRLRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDAGRERQDIVTLVKRQLTREISEGRFTANREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            7983..8267
                     /locus_tag="ACHOUK_003731"
     CDS             7983..8267
                     /locus_tag="ACHOUK_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFP9899096.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAWADSLSTDNPLPVPR"
     gene            8267..8542
                     /locus_tag="ACHOUK_003732"
     CDS             8267..8542
                     /locus_tag="ACHOUK_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9899097.1"
                     /translation="MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIYVLRLWHTRENR"
     gene            8637..8843
                     /locus_tag="ACHOUK_003733"
     CDS             8637..8843
                     /locus_tag="ACHOUK_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899098.1"
                     /translation="MNYDEITKITAERISDYMTEAVNTDSIAVAEMFHNAAWGVRTLW
                     FELVTKIDIDIHKKNRYASYDLDR"
     gene            complement(8943..9158)
                     /locus_tag="ACHOUK_003734"
                     /pseudo
     CDS             complement(8943..9158)
                     /locus_tag="ACHOUK_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(9202..9768)
                     /locus_tag="ACHOUK_003735"
                     /pseudo
     CDS             complement(9202..9768)
                     /locus_tag="ACHOUK_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
     gene            complement(9780..10184)
                     /locus_tag="ACHOUK_003736"
                     /pseudo
     CDS             complement(9780..10184)
                     /locus_tag="ACHOUK_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(10184..10369)
                     /locus_tag="ACHOUK_003737"
     CDS             complement(10184..10369)
                     /locus_tag="ACHOUK_003737"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899099.1"
                     /translation="MDVRNKKLVFWFVRVDDEGYPEIARCTEREFATILAGISAGGMY
                     CPECGTVHWPDGVPPPF"
     gene            10546..12756
                     /gene="katP"
                     /locus_tag="ACHOUK_003738"
     CDS             10546..12756
                     /gene="katP"
                     /locus_tag="ACHOUK_003738"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase KatP"
                     /protein_id="HFP9899100.1"
                     /translation="MIKKTLPVLILLALSGSFSTAVAADKKETQNFYYPETLDLTPLR
                     LHSPESNPWGADFDYATRFQQLDMEALKKDIKDLLTTSQDWWPADYGHYGPFFIRMAW
                     HGAGTYRTYDGRGGASGGQQRFEPLNSWPDNVNLDKARRLLWPVKKKYGSSISWGDLM
                     VLTGNVALESMGFKTLGFAGGREDDWESDLVYWGPDNKPLADNRDKNGKLQKPLAATQ
                     MGLIYVNPEGPGGKPDPLASAKDIREAFSRMAMDDEETVALIAGGHTFGKAHGAASPE
                     KCIGAGPDGAPVEEQGLGWKNKCGTGNGKYTITSGLEGAWSTSPTQFTMQYLKNLYKY
                     EWELHKSPAGAYQWKPKKAANIVQDAHDPSVLHPLMMFTTDIALKVDPEYKKITTRFL
                     NDPKAFEQAFARAWFKLTHRDMGPAARYLGNEVPAESFIWQDPLPAADYTMIDGKDIK
                     SLKEQVMDLGIPASELIKTAWASASTFRVTDYRGGNNGARIRLQPEINWEVNEPEKLK
                     KVLASLTSLQREFNKKQSDGKKVSLADLIVLSGNAAIEDAARKAGVELEIPFTPGRTD
                     ASQEQTDVASFSVLEPTADGFRNYYSKSRSHISPVESLIDKASQLDLTVPEMTALLGG
                     LRVMDINTNNSSLGVFTDTPGVLDNKFFVNLLDMSTRWSKADKEDTYNGFDRKTGALK
                     WKASSVDLIFSSNPELRAVAEVYASDDARNKFIHDFVKSWNKVMNSDRFDLNNK"
     gene            12800..13189
                     /locus_tag="ACHOUK_003739"
     CDS             12800..13189
                     /locus_tag="ACHOUK_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFP9899101.1"
                     /translation="MQKSNKVIISCGMALYFLCGGITVAEELDESMQVLARNMSVLQS
                     SMDKKELLEALDRMDDAVDESMKRLPESISPADEEGKNDYIKELQKLEKEIMLAKQKV
                     LSGQLSDIPDSVNKMNKIRVEGHDKFR"
     gene            13246..13402
                     /locus_tag="ACHOUK_003740"
                     /pseudo
     CDS             13246..13402
                     /locus_tag="ACHOUK_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558160.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    13302..>13402
                     /locus_tag="ACHOUK_003740"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT         3544 a         3074 c         3637 g         3147 t
ORIGIN      
        1 cggattgccg gtatcagcag ggatgacggg ctgataactc tgcaggatgc ggaaggtaac
       61 acgcggctga tttcgccccg ggaggcggtg gctgaaggcg tcacactgta ccccccggac
      121 accatcaggg tggggaccgg tgaccggatg cgcttcacga agagtgaccg ggggcgtggt
      181 tatgtggcca acagcgtctg gacggtgaca gcggtttccg gtgacagtgt cacgctgtcg
      241 ggcggacagc agacccgggt gattcgcccc gcccaggagc gggcagagca acatattgac
      301 ctggcctatg ccatcaccgc tcacggtgcg cagggggcaa gtgaaacctt tgccatcgcg
      361 cttgaaggta cggaaggcag ccggaaactg atggccggct ttgagtcagc ctacgtggcc
      421 ctgtcgcgta tgaagcagca tgtgcaggtg tacaccgata accgtcaggg ctggacggat
      481 gccattaaca atgccgtaca gaaaggaaca gcccacgatg tgtttgaacc gaaaccggac
      541 cgggaggtca tgaatgcaga gcggctgttc agtacggcgc gggagctgcg ggacgtggcg
      601 gcagggcgtg ccgttcttcg tcaggcgggg cttgccgggg gagacagtcc tgcacggttt
      661 attgctccgg gacgtaaata tccacagccg tatgtggcac tgccggcgtt tgaccgtaac
      721 ggcaagtctg ccggtatctg gctgaacccg ctgaccacgg atgacggaaa cgggctgcgg
      781 ggattcagtg gtgaaggacg ggtgaaaggc agcggggatg cgcagttcgt ggccctgcag
      841 ggcagccgta acggagagag cctgctggct gataatatgc aggatggtgt ccggattgcc
      901 cgtgataatc ctgacagcgg tgtggtggtg agaatcgccg gtgaaggtcg tccgtggaat
      961 cccggtgcca tcaccggtgg tcgcgtgtgg ggggatatcc cggacaacag cgtccagccg
     1021 ggagccggaa atggcgagcc ggtcacggca gaggtactgg cacagcggca ggctgaagag
     1081 gccatccgcc gtgaaacgga acgccgcgca gatgaaattg tccgtaaaat ggcagagaac
     1141 aaacctgacc tgccggacgg cagaacagag caggctgtca gggagattgc cgggcaggag
     1201 cgtgaacggg ctgtcacttc tgaacgggaa gccgcgctgc cggagagtgt actgcgtgaa
     1261 ccacaacggg agcgggaggc ggtccgtgag gttgtccggg aaaacctgct gcaggagcga
     1321 ctgcagcaga tggagcggga tatggttcgt gacctgcaga aagagaaaac cctgggcgga
     1381 gactgataca ggaagacaaa cactgatgac aacggataac acgaacacga cacgtaacga
     1441 ttcactggct gcccggaccg atacctggtt gcagtcgttg ctggtctggt cacccggaca
     1501 gcgggatatc atcaaaacgg tggcactggt gctgatggta ctggaccacg caaaccgcat
     1561 tctgcatctg gaccagacgt ggatgtttct ggccgggcgt ggagcctttc cgttgtttgc
     1621 tctggtgtgg gggctgaatc tgtcccgcca tgcgcatatc aggcagtcgg ccattaaccg
     1681 gctgtgggga tggggaatta ttgcccagtt cgcgtattat ctggcaggct ttccgtggta
     1741 tgaggggaat atcctgtttg cctttgcagt ggcggcacag gtgctgacgt ggtgtgaaac
     1801 gcgcagtggg tggcgtacag cagcggccat tctcctgatg gcactgtggg ggcctttgtc
     1861 cggcaccagt tacggcattg ccgggctgct gatgctggca gtcagccacc ggctgtaccg
     1921 ggcggaagac agaacggaac gtctggtgct ggttgcatgc ctgctggccg tgattccggc
     1981 acttaacctt gcctccagtg atgcggcggc tgtagctggt cttgtgatga cggtgctgac
     2041 cgttggtctg gtgtcctgtg cagggaaatc attaccccgt ttctggcccg gagatttttt
     2101 cccgacgttc tatggctgtc atctggctgt gctgggcatt ctggcgttgt gactggtatg
     2161 atactgacgc tgattctgct tattaataaa caacataaca caggatattt atgaaaatta
     2221 cggatcacaa actctctgaa ggtattgccc tgacatttcg tgttcctgaa ggaaatataa
     2281 aacatccttt gattatctta tgccatgggt tttgcggtat caggaatgtt ctgctccctt
     2341 gctttgcgaa tgccttcaca gaagcaggat tcgcaacgat tacttttgat tatcgaggat
     2401 tcggtgagag tgatggagaa cgagggcgtc tggttcctgc aatgcagacc gaagatatta
     2461 tttcagttat caattgggca gaaaagcagg aatgtattga taatcagcgt attgggttat
     2521 ggggaacatc gctgggaggg gggcatgtct tcagtgcagc tgcgcaggac caacgcgtaa
     2581 aatgtattgt cagccagctg gcttttgctg acggtgatgt tctggttacc ggagagatga
     2641 atgaatcgga gagagcgtct tttctatcca cgctgaataa aatggcagag aagaagaaaa
     2701 acaccgggaa agagatgttt gtgggggtta cacgggtact aagtgatgat gaatcgaagg
     2761 tattcttcga gaaaatcaaa gcccggcatc ccgaaatgga tataaaaata cctttcctta
     2821 cggtcatgga aacgttgcaa tataaaccgg cagagagtgc agccagcgtc cagtgcccag
     2881 ttttggttgt tattgccggc caggatagcg ttaatccacc ggaacaggga agagcattat
     2941 atgatgctgt tgcttctggt actaaggaat tatacgagga agctgatgca tgccattatg
     3001 atatttatga gggcgcgttt tttgaacgtg tagtagctgt gcagacacaa tggtttaaac
     3061 aatatcttta attgtcagta tttatacgtt aaaggtcgtt gaaattaact tcctgttacg
     3121 atgagccgtt tcaacggcct gcagggcata ttttcacgaa ggtcagagta agcatggcag
     3181 agcaaaaacg accggtactg acactgaagc ggaaaacgga aggagagacg cctgtccgca
     3241 gccggaaaac catcatcaat gtcaccacgc caccaaaatg gaaggtgaaa aagcagaagc
     3301 tggccgagaa agcagcccgg gaagcggagc tggcggcaaa aaaagcgcag gccagacagg
     3361 cgctgtccat ttatctgaac ctgcccacgc aggatgaggc cgtgaacacc ctgaaaccct
     3421 ggtggccggg attatttgac ggtgacacgc cccggcttct ggcctgcggt atccgggacg
     3481 tgttactgga agacgtggcg cagcggaata tcccgctctc gcataaaaag ctgcgcaggg
     3541 cgctgaaggc catcacccgt tcagaaagct atctgtgtgc catgaaagcc ggtgcctgcc
     3601 ggtatgacac ggaagggtat gtgacggagc atatttctca ggaggaggaa gcgtatgcgg
     3661 cagcgcgtct ggataaaatc cgccgccaga accggataaa ggcagaactt caggccgtgc
     3721 ttgatgagaa ataaaaaaag cctccctcag agagaggagg cagggaaata aggcttaaaa
     3781 ggaaataact tccgtacccg aaaggaagcc aattcagtag attatttaac accataatac
     3841 tgacgggtta aagaggagtg ataaagatga atggattcag aaacagttca cggaacggtc
     3901 aggtctggcg ttaccagcgt gccggaagcc gggccgttat tctggaagtc agtggacgct
     3961 ggatggaagc ggcagaagca tggcgacggg ctgccggtgt tgcaccccgg actgactggc
     4021 agcagtttgc ccgaaaaagg gctgaacact gccaccggcg ctgccgagga agggggtaac
     4081 gaaaaaagca gccttcatta ctccagggat tcagaaggct gaataatgca tgagatttgc
     4141 tttttttaat atccggtcgg gcagtatgac cggccagttc tgagtagcac agagtttttt
     4201 ccgtattaag tcgtgatttt atattttgtg attaatttca caaaataagg tgttattaag
     4261 tgtgtgctgc aatattcagg atgcctgaaa tacaggtgct atttatctga tacggagaat
     4321 aacatgagga aatatattcc actggtatta tttatctttt catggccggt attgtgtgca
     4381 gatattcatg gacgggttgt tcgtgtactg gacggtgata ccatcgaggt catggactca
     4441 cggaaggcgg tgcggatcag gctggtaaat attgatgcgc cggaaaagaa acaggattat
     4501 ggccggtggt cgacagacat gatgaaatca ctggttgcag gaaaaacggt taccgtcaca
     4561 tattttcagc gagatcgtta tggtcgtatg ctcgggcagg tttatgcacc agacggaatg
     4621 aatgttaatc agtttatggt ccgggccggg gccgcctggg tatatgagca gtacaacaca
     4681 gatcccgtgc tgcctgtcct tcagaacgaa gccagacaac aaaaacgtgg gctctggtca
     4741 gatgctgatc cggttccccc ctggatatgg agacaccgta aataaagcga tggcaatctt
     4801 atcattcatc tgttctcagc gggaataaaa atggaaaaaa caaaacagga atggctgtat
     4861 caattacggc gatgttcatc agtcaatacg ctggaaaaaa ttattcataa aaacagagac
     4921 agtttatcga ccagtgaacg tgaatcattt aattcagcag cagaccaccg gttagcagaa
     4981 ttaatcaccg gaaagttata tgacaggata ccaaaagaaa tatggaaata tgtaaggtaa
     5041 tagttcagca taattaatta ccaggagcag ataaacaatg aagctgatta tttttattct
     5101 cattgttctg attattgcag cgttacttat ccggattatc ctcagaagtg taaatcagca
     5161 ttctcctttg ctgatgcaat tgcatgcggc aggtatacgg accggtgatg cagagcgaat
     5221 actgtccggc ggtgaatatg atgccagcca tttacgacct gaacgcagac gccgggatat
     5281 tcttctggaa gaggttctga ggcaggctgg tattccgttg ctcagaagcc acgatgccag
     5341 aaaactgctg cagatgaccg gagaatggct gaatacaaca ggggctgctc agcagtcacc
     5401 ggaacatcgt agctgacgcc ttcgcgttgc tcagttgtcc aaccccggaa acgggaaaaa
     5461 gcaagttttc cccgctcccg gcgtttccat aactgaaaac catactattt cacagtttaa
     5521 atcacattaa acgacagtaa tccccgttga tttgtgcgcc aacacagatc ttcgtcacaa
     5581 ttctcaagtc gctgatttca aaaaactgta gtatcctctg cgaaacgatc cctgtttaag
     5641 tattgaggag gcgagatgtc gcagacagaa aatgcagtga cttcctcatc tggcgcaaaa
     5701 cgagcataca gaaaggggaa tccgctttct gatgcagaga aacaaagatt atcagtggcc
     5761 cgtaaaagag cttcgttcaa ggaagtaaaa gtatttcttg aaccaaagta taaggccatg
     5821 ctcatgcaaa tgtgtcatga agatggcctg actcaggctg aagttctgac cgcactgata
     5881 aaaagtgaag cgcaaaaacg atgtgtgtga tgatgggctt acattcttga gtgttcagaa
     5941 gattagtgct agattactga tcgtttaagg aattttgtgg ctggccacgc cgtaaggtgg
     6001 cagggaactg gttctgatgt ggatttacag gagccagaaa agcaaaaacc ccgataatct
     6061 tcagaaagtt tggcgactga gaagattacc ggggcccact taaaccgtat agccaacaat
     6121 tcagctatgc ggggagtata gttatatgct cggaaaagtt caagacttct ttctgtgctc
     6181 gctccttctg cgcattgtaa gtgcaggatg gtgtgactga tcttcaacaa acgtattacc
     6241 gccaggtaaa gaacccgaat ccggtgttca caccccgtga aggtgccgga acgctgaagt
     6301 tctgcgaaaa actgatggaa aaggcggtgg gcttcacctc ccgttttgat ttcgccattc
     6361 atgtggcaca tgcccgttcg aagggactgc gtcggcgcat gccaccggta ctgcgtcgcc
     6421 gggctattga tgcgctgctg caggggctgt gttttcacta tgacccgctg gccaaccgtg
     6481 tccagtgctc catcaccaca ctggccattg agtgcggact ggcgacggag tctgctgccg
     6541 gaaaactctc catcacccgt gccacccggg ccctgacgtt cctgtcagag ctgggactga
     6601 ttacctacca gacggaatat gacccgctta tcgggtgcta cattccgacc gacatcacgt
     6661 tcacaccggc tctgtttgct gcccttgatg tgtctgagga tgcagtggca gctgcgcgcc
     6721 gcagccgtgt tgaatgggaa aacaggctgc gcaaaaagca ggggctggat accctgggta
     6781 tggatgaact gatagcgaaa gcctggcgtt ttgtgcgtga gcgtttccgc agttaccaga
     6841 cagagcttaa gtcacgggga ataaagcgtg cccgtgcgcg tcgtgatgcg ggcagggaac
     6901 gtcaggatat cgtcaccctg gtgaaacggc agctgacgcg tgaaatctcg gaagggcgct
     6961 tcactgctaa tcgtgaggcg gtaaaacgcg aagtggagcg tcgtgtgaag gagcgcatga
     7021 ttctgtcacg taaccgcaat tacagccggc tggccacagc ttccccctga aagtgacctc
     7081 ctcagaataa tccggcacgc gccggaggca ttcgcacgcc tgaagcccgc cggtgcacaa
     7141 aaaatcagca ccacatccca aaaacaacct catcatctac cttctggtgc atccggtccc
     7201 ccctgttttg atacaaaaca cgtctcacag acgggcaatt ttgtttatcc acattaaact
     7261 gcaagggatt tctctgtaag attacaaccg ttcatgtcat aaagcaccag ccgccaggct
     7321 tacagggtgc aatgtatctt ttaaacaccc gtttatatct cctttaaact acttaattac
     7381 attcatttaa aaaaaagact tattcactgc ctgtcctgtg gacagacaga tatgcacctc
     7441 ccaccgcaag cggcgggccc caaccggagc cactttagtt acaacacaca aaaacaacct
     7501 ccagaaaaac cccggtcccg cgcagaactg aaaccacaaa gcccctccct cataactgaa
     7561 aaacggcccc gccccggccc aaagggccgg aacagagtcg cttttaatta tgaatgttgt
     7621 aactaagcat catcatcgct gtcagtcttc tggctgaacg ttctcagtac acgctcgtaa
     7681 gcggccctga gggctcgcta acgcggagat acgccccgac ttcgggtaaa ccctcgtcgg
     7741 gaccactccg accgcgcaca taaactctct catggctgaa agcgggtatg gtctggcagg
     7801 gctggggggg gggtaaggtg aaatccatca atcagtaccg gcttacgccg ggcttcggcg
     7861 gttttacacc tgtgctacat gtaacctccg tataccgcct tacatgcgct gacgcgcatt
     7921 atttgccttg ttccgtctga aagtaatcac tatgattaaa tatgattaac gactaatcgg
     7981 atatgcaaat gaaaaacaat accgcacaag caacaaaagt cattaccgcg catgtgccat
     8041 tacctatggc tgataaagtc gaccagatgg ccgccagact ggaacgctcc cggggttggg
     8101 ttatcaaaca ggcgctttct gcatggcttg cccaggagga ggagcgtaat cgcctgacgc
     8161 tggaagccct ggacgatgtg acatccggac aggttattga ccatcaggct gtacaggcct
     8221 gggcggacag cctcagtact gacaatccgt taccggtgcc acgctgatgg aactgaagtg
     8281 gagcagtaag gcgctttctg atttggcgcg gttatatgat tttctggtgc tagccagtaa
     8341 acctgctgcc gccagaacgg tacagtccct gacacaggca ccggtcattc tgttaactca
     8401 tccacgtatg ggggaacagt tatttcagtt tgaacccagg gaggtcagac ggatttttgc
     8461 tggcgagtac gaaatccgtt acgaacttaa tggccagact atttatgtat tgcgcctgtg
     8521 gcacacacga gaaaacaggt agattgtgtg tggggacaac ttaacatagc ctgcagatat
     8581 taattctgga acattattat caggccagat atttaaataa tcttgtagga gataaaatga
     8641 attacgatga aataacgaaa attaccgctg aacgtattag cgactacatg actgaagcag
     8701 ttaatacaga ctctatagcc gttgcggaaa tgtttcataa cgcagcatgg ggagtgcgaa
     8761 cattgtggtt tgaactcgtc acaaaaatag atatagatat acataaaaag aacagatatg
     8821 cgagttatga tcttgatcga taggaattta aatcccaaaa agattaaaaa aacacccaaa
     8881 aacggatgtt tattcaacac tgcctgcgtg ccatgagtgc accgccgtca attaaatgga
     8941 tctatttcat cctgaccagc atagtgctcc agacggctca tcggcaccgg cggttttttc
     9001 aggtaagagc cgaagtacac cgccacatgt gtggcgttat ccatcacccg ggatacgttg
     9061 acattccagc cccgccggta atgactgtcc aggaagcgat tccagtcccg tttactgctt
     9121 ccttctgctg ccagcgcatc cggtatcacc aggtccggtg aggtgtactg gcaatagcgg
     9181 acactaccat ttgttctttt tttaagcagc catctgatga tatttttccc tgaaggctgc
     9241 cggggagata ttccccagac gagagtgacg acgctgacga ttgtagaaaa tctcaatgta
     9301 ttcccgtatt actgagatgg cttcatcccg gttattaaaa cgatagtggc tcaggctctc
     9361 atttttcagc gttccccaga agctttccat cggagcgttg tcgtaacagt tacctttacg
     9421 cgacattgat gttttcagac caaactgctc ctgtatgacc cggtaatcgt atgcgcagta
     9481 ctgtgaacct cgatcagagt ggtggattag cccggcaggt gggcgctggc tcctgagcgc
     9541 cataaacagg gctttacctg tcagctcttt tgtcatgcgc tctcccatgg cgtagccgac
     9601 aatttcgcac gtataaacat ctttgatgcc agcgaggtac aaccatccct cctgtgtggc
     9661 aacatacgtc aggtccgcca cccagacctg atttggtgct gcaggagcga acgtctggtt
     9721 cagcagattt ggcgcaactg gcagattgtg gttcgagttc gtagtcgcac atctgcctag
     9781 cggcagattt cctgtatcac atccgcagca atgcggctca tctctgccag taaccaccgg
     9841 ttgtggaaca ccagggacca gtactggcag ggaagtgtga acacaatatg ctgccacgga
     9901 cagtcgggaa ccagactcag cagatactgt atccactgtg cgccagcctt cactccgcag
     9961 tgcgggcagg agcgactttt acaccggaag cagacctttt ttatgtggct gcagtccgga
    10021 gatgaacagc accactgtgt gtaccccatc agcgtggtcc cgcaggccat gatttttgtc
    10081 acagactcaa tgactaccgg acgcactgaa ccttccggtt gtttctccag ccagttaagc
    10141 cagcggtttc cctgctgaaa aatgtcggca aaacggggaa gcatcagaag ggcgggggaa
    10201 ctccgtccgg ccagtgaacc gtgccacact ccgggcagta catgccgccg gcgctgatac
    10261 cggcaagaat ggtcgcaaac tcccgctccg tgcagcgggc tatttcagga tacccttcgt
    10321 catcaacacg tacaaaccag aagaccagct ttttgtttct gacatccaca aagaagggaa
    10381 tattcaggtc tgcgcagcac tcaacggcat cgtcagttgc ggcttggaac cccttagtat
    10441 tttttgtctg tagtatctat cccagcaata ggtatatcct gttgcatcaa taaagttgac
    10501 ttttgtatac aacatgcgaa tttcccttaa tccggagcta ttcgtatgat aaaaaaaact
    10561 cttcctgttc tgattcttct ggcgctatcg gggagctttt ctaccgctgt agccgctgat
    10621 aaaaaagaga ctcaaaattt ctactatcca gaaacactgg atttaactcc tctgagatta
    10681 cacagccctg aatcaaatcc ctggggggct gattttgatt atgccaccag atttcaacag
    10741 ctggatatgg aggctctgaa aaaagatatc aaagatttgc tgacaacttc ccaggattgg
    10801 tggcctgcgg attatggtca ttatggtcct ttctttattc gtatggcttg gcacggtgcc
    10861 ggaacataca ggacatatga tggccgggga ggcgccagtg gtggtcagca acgttttgaa
    10921 ccgctgaaca gctggccgga taacgttaat ctggataaag cccgtcgatt gctgtggcca
    10981 gtcaagaaaa aatacggctc cagtatttcc tggggagacc tgatggtcct gactggtaat
    11041 gttgcccttg aatccatggg atttaaaacg ctgggatttg ctggcggaag agaagatgac
    11101 tgggagtcgg acctggtata ctgggggcct gacaacaagc ctcttgcaga taaccgggat
    11161 aaaaacggga aacttcagaa acctcttgcc gccacgcaga tgggacttat ttatgtcaat
    11221 cctgaaggcc ccggtggaaa accagatcct ctggcttccg cgaaagatat cagggaagct
    11281 ttttcacgta tggccatgga tgatgaggag actgtggccc tgatcgcggg agggcataca
    11341 tttggtaaag cacatggtgc agcgtctcct gaaaaatgta ttggcgcagg gcctgatggt
    11401 gcacctgtgg aggagcaggg actgggatgg aaaaataaat gtggtacagg aaacggcaaa
    11461 tataccatca ccagtggcct ggaaggagcc tggtcgacat cgccaaccca gttcacaatg
    11521 cagtatctga agaatttata taaatatgaa tgggagctgc acaagagtcc tgccggtgct
    11581 tatcagtgga agcctaaaaa agcggcaaat atagttcagg acgcgcatga tccgtctgtc
    11641 ctgcatccgt tgatgatgtt tacgacggat attgctctta aagttgatcc tgaatataag
    11701 aaaataacca cccgtttcct gaatgatcca aaagcttttg agcaggcatt cgcaagagca
    11761 tggtttaaac tgacccaccg ggatatggga ccggcagccc gatatcttgg taatgaagtt
    11821 cctgcagaat catttatctg gcaggatcct cttcctgcgg cggattatac aatgattgat
    11881 ggtaaagaca ttaagtcgct gaaagagcag gttatggatt tgggtatccc tgcatctgag
    11941 ctgataaaga cagcctgggc ttcagcttcc acatttcgtg tgactgatta tcgtggggga
    12001 aataatggtg cccgcatcag gttacagccc gaaattaact gggaagttaa tgagcctgaa
    12061 aaactgaaga aagtactggc atccctgacc tcattacagc gtgaatttaa caaaaaacag
    12121 tctgacggaa agaaagtgtc gttggctgat ttaattgttc tttcgggtaa tgctgcaatc
    12181 gaagatgcgg ccagaaaagc cggggtggaa cttgagattc cctttactcc gggaagaact
    12241 gacgcctctc aggagcagac ggatgttgcc tcattcagtg tactggagcc gacagcagat
    12301 ggattcagaa attattactc aaaaagcaga agtcatatat cgccggttga aagcctcatt
    12361 gataaagcca gtcagctgga tctcaccgtt cctgaaatga cggcattact gggtggtctg
    12421 cgggtaatgg atattaatac aaataattct tcgttgggag tgtttaccga tacccctggt
    12481 gttctggata acaagttttt tgttaatctg ctggatatgt caacacgatg gagtaaagca
    12541 gataaagaag atacatacaa tggattcgat cgtaaaacgg gagcattaaa atggaaagca
    12601 tcctctgttg atttaatctt cagttcaaat cctgaattac gtgcggtggc agaagtatat
    12661 gcctcggatg atgcgagaaa taagtttatt catgattttg ttaaatcgtg gaataaagtt
    12721 atgaatagcg atcggtttga tttaaacaat aaatgataaa actgatgagg gagggcacct
    12781 ccctcatgaa ggagaaatta tgcagaaaag caataaagtt ataatcagct gtggtatggc
    12841 attatatttt ttgtgtgggg ggattacagt ggcagaagaa cttgatgaaa gcatgcaagt
    12901 tcttgcaagg aatatgagtg tacttcagtc atcaatggat aaaaaggagc ttcttgaagc
    12961 tcttgacaga atggatgatg ctgtagatga gtcaatgaag cgtcttcctg agagcatttc
    13021 accggctgat gaagaaggga agaatgacta tataaaagaa cttcaaaagc ttgaaaaaga
    13081 gattatgctg gcgaaacaaa aagtcttatc gggacagtta tcagatatac ccgattcggt
    13141 aaacaagatg aataaaatca gagttgaggg gcatgataaa ttcagataaa tcagttggtt
    13201 gattcctgcg ggacgaatac atacgtcagg aacataactt tatggctgga acgtgaaaat
    13261 cgtgaactgc gccgcagtaa cgatatcctt cgccaggctt ccgcttattt tgcgaaggcg
    13321 gagttcgacc gcctctggaa aaaataatgc cactgctgga taagctgcgt gagcagtacg
    13381 gggtcggacc ggtatgcagt ga
//
LOCUS       DAZKIP010000118        13392 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZKIP010000118 DAZKIP010000000
VERSION     DAZKIP010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.119"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..815
                     /locus_tag="ACHOUK_003741"
     CDS             <1..815
                     /locus_tag="ACHOUK_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFP9899102.1"
                     /translation="NVLQSQIITDNHFLHHPKVESELTRKYERARLDTENIYLLPLAR
                     GNNHNYDGKSVVEIRKLDISKESWPFNYVTEACRESDGITTTGRMLYRNLKITSALDE
                     IYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYAGTTIGY
                     ISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIELGNDPIIGI
                     EAYPGTAEIHAKMGYKVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK"
     gene            complement(956..1729)
                     /gene="uxuR"
                     /locus_tag="ACHOUK_003742"
     CDS             complement(956..1729)
                     /gene="uxuR"
                     /locus_tag="ACHOUK_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFP9899103.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1944..3404)
                     /locus_tag="ACHOUK_003743"
     CDS             complement(1944..3404)
                     /locus_tag="ACHOUK_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFP9899104.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3485..4669)
                     /gene="uxuA"
                     /locus_tag="ACHOUK_003744"
     CDS             complement(3485..4669)
                     /gene="uxuA"
                     /locus_tag="ACHOUK_003744"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFP9899105.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SH"
     gene            5009..6352
                     /gene="gntP"
                     /locus_tag="ACHOUK_003745"
     CDS             5009..6352
                     /gene="gntP"
                     /locus_tag="ACHOUK_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFP9899106.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6526..7428)
                     /gene="fimH"
                     /locus_tag="ACHOUK_003746"
     CDS             complement(6526..7428)
                     /gene="fimH"
                     /locus_tag="ACHOUK_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFP9899107.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNQGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7448..7951)
                     /gene="fimG"
                     /locus_tag="ACHOUK_003747"
     CDS             complement(7448..7951)
                     /gene="fimG"
                     /locus_tag="ACHOUK_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HFP9899108.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(7964..8494)
                     /gene="fimF"
                     /locus_tag="ACHOUK_003748"
     CDS             complement(7964..8494)
                     /gene="fimF"
                     /locus_tag="ACHOUK_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFP9899109.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8504..11140)
                     /gene="fimD"
                     /locus_tag="ACHOUK_003749"
     CDS             complement(8504..11140)
                     /gene="fimD"
                     /locus_tag="ACHOUK_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFP9899110.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAVQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRSSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLARNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(11207..11932)
                     /gene="fimC"
                     /locus_tag="ACHOUK_003750"
     CDS             complement(11207..11932)
                     /gene="fimC"
                     /locus_tag="ACHOUK_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313302.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFP9899111.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     IIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGARVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(11969..12508)
                     /locus_tag="ACHOUK_003751"
     CDS             complement(11969..12508)
                     /locus_tag="ACHOUK_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9899112.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTPPGGNMQFQGVIIAETCL
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(12573..13121)
                     /gene="fimA"
                     /locus_tag="ACHOUK_003752"
     CDS             complement(12573..13121)
                     /gene="fimA"
                     /locus_tag="ACHOUK_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFP9899113.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAAATTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLAQDGATSSAVGFNIQLNDCDTNVASKAAVAFLGTAID
                     AGHTNVLALQSSAAGSATNVGVQILDRTGAALTLDGATFSEQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
BASE COUNT         3238 a         3454 c         3251 g         3449 t
ORIGIN      
        1 gcaatgtcct tcagagtcaa attattacag ataatcactt ccttcatcat ccaaaagtcg
       61 aaagtgagtt aacacgtaaa tatgaacgtg cacgcctgga tacggaaaat atctatcttc
      121 tgcctcttgc tcgtggcaat aaccataatt acgatggaaa aagcgttgtc gaaattagaa
      181 aacttgatat tagtaaagaa tcctggccat ttaattatgt gacagaagca tgcagagaat
      241 ccgatggtat tacaacaaca ggacgtatgc tctatcgaaa cttgaaaatc acctcagctt
      301 tagacgaaat atatggtggt atatgtaaaa aggcgcatgc tgctacagag ttagcagaag
      361 gtttacggct aaatttattt atgaagagcc ctttcgatcc tgttgaggac tatacagttc
      421 atgaaataac attaggccct ggttgcaatg ttccaggtta cgccggaacc acgataggat
      481 atatttcaac ccttccagct tctcaggcaa aaagatggac caatgagcag ccaagaattg
      541 atatctatat tgatcagata ataactgtct caggggtagc aaattcttcc ggattcgccc
      601 ttgccgcatt acttaatgct aatattgagt tgggtaatga cccaataatt ggtatagaag
      661 cctatccagg taccgcggag atccatgcaa aaatgggata taaagttatc cccggtgatg
      721 aagatgcgcc actcaaacgc atgactctgc aaccgtcatc tcttccagaa ctgtttgaat
      781 taaaaaatgg tgaatggaat tatattggca aataaccact gagaagaatg agtactaaca
      841 ttcaggttat atagatattt aaacaatacc ctgcaattta caaagtaata ctcattgcga
      901 cttcttaatt taagaaagcg caaggaacgt ttacccttgc gcttattata ataagtcagg
      961 cgtcgacttt atccagcggc catgaatcaa acagatagcc gtcaaaataa atatcgtcaa
     1021 cgttcgagaa ttccagcaga cgttgcttaa cgttttccag atgctgccac attgccagct
     1081 tcgccgctcg ggcatctttt ttgattaacg cggcgaggat ctgtttgtga tcgcccaacc
     1141 actctttgcg atacaggctg tcatccagat ggctgtgcaa ctggatccac attgggttgt
     1201 tttcccgcca ctgccaggac tgacggaaca gctccaccag catgctgtta tgcgttgctt
     1261 cggcaatagc gagatggaac tgcatgtcac cgctttcgct gctgcccggc gcactggaag
     1321 ccagttcacg ctcttccagt tgcaatgcct gacgcatttt gacgatatct tcgcgggtag
     1381 cctgcagggc ggcaaactcg gcgatgttac tttccagtaa ctgacgcgcc tgtaacagct
     1441 caaaaggacc cgcatcattg cagacgttgg catccggact gtctgtgttg tggctgcctg
     1501 agctatcaag aacatagata ccggcacccc ggcgcacttc caccagccct ttgatctcca
     1561 gcatgatcag cgcttcacgt accaccgtcc gcgtgacatc aagcatttct gcaatttcac
     1621 gctccggcgg tagccgttca ccaggattgt acggcgtctt tatgatcaga tcgcggatca
     1681 tcgccccgac ttcctggtaa ggtctttgcg cagaggtggc agatttcatg gtgttgtgtc
     1741 cgttaggtgc ggttaatcaa atttttgccg gatgcggcgt gaacgcctta tccggcctac
     1801 aacgggcaat tttacggccc ccgttaatct caaactatac cattagcact ggaagttggc
     1861 acgtacaggt gggcaaaaaa acgaaaactg gtcaaccaaa tatcagtttg attgaccagt
     1921 aagtctgtag ggtaatcagt tagttacagc gcagccacac actcgcgcgc accgcgttcg
     1981 cacagctgct gatatgccgc agtcaccgcg ccaacaaagt cggcattctg cggcagatca
     2041 tcggcaaaaa taccgctcag gcccagcagc gctttcacgc ggtctgcgcc ctgatactgc
     2101 gcgttaatct tctggaactc ggccagcatc gggtcgacca cgtcaatggc attaccctgc
     2161 tcatccacgc cctgggtgta acgcatccag ccagccacgc ccagcgccag gtgacgccag
     2221 ctgccgccgt tttgcaggtg cagacgcacc gggtccagca gacgctgcgg taacttctgg
     2281 ctgccgtcca tcgcaatctg ccaggtacgg tgacgcagag acgggttgct gaaacgctcg
     2341 atcagcagcg tcgcataggc gttcaggtcc gtaccttccg gcatcgacag cgttggcgct
     2401 tgttcctgca tcatcagggc aaaggccgct ttgcgataat ccgggttagt catggtgtcg
     2461 gcaatagttt catagccgcc gaggtaaccg aggtacgcca ggaaagagtg gctgccgttc
     2521 agcatacgca gtttcatcat ttcgaacggc acaacgtctg caacgaactg tgcgcccact
     2581 ttatcccagt ccggacgacc attaacgaag ttatcttcaa taacccactg acggaacggt
     2641 tcgcaggcaa tggcgcacgg gtcgtaaaca cccagctggt cagcaatttc ctgtaaggtt
     2701 tctggcgtcg ccgccggaac gatgcggtca accatggtgc acgggaaggt gacgttctct
     2761 tcaatccatg ccgctagctg cgggtcacgc gcctgagcca gccccagtac cgcgaccttc
     2821 gccacatgac cgttttcacg cacgttatcg caggacatca ccgtaaatgc tttcagccct
     2881 ttttcacgac gcagacgcaa ggcttcgacg atgtaaccaa tcgcggactt cggcgcagtc
     2941 gggttttcca gatcgtgctt gatcagcggg ttattgagat ccagttgacc gcttgccgca
     3001 tcagcgcagt agcctttttc cgtgaccgtc agggagacaa tcgccgtttg cggacgcgcc
     3061 atcgcgttga gaataccttc gcagccgtcg atttccgggt gcagcgcttc tttcatcgaa
     3121 ccgataattt tcagctcggt gctttccgcg cctttttccg ccacggtgta cagcagttgc
     3181 tgttttttca ggttttcgat cagcacgcgg tcgttgcctg gcatcaggtt aacttcgcag
     3241 ataccccagt cgctgtcggt gctttccagc agatgatggg tgtacagcgc ctggtgcgca
     3301 cggtgaaacg ccccgcaacc gagatgcaca atgcgtgatt ccagacgaga atgatcccat
     3361 gacgggcggg caaccggcag attgctgtca acaatagtag tcatcgtaaa ctccagaatc
     3421 cgtcttcaga gagtgacggc aaggaagaag gagccggcac ggcgcgcagc catgccggtg
     3481 ggtattaatg gctaaagaaa gcgcgctgga tcgccagttc gacaccgcga acttcggcca
     3541 ggcctttcag acgaccaatt gcggagtaac cagggttggt tttcttcttc aggtcgtcca
     3601 gcatctgatg accgtggtcc ggacgcatcg ggatcaggtc ttctttgcct tccgctttac
     3661 gacggtgttc ttcttcaaca atcgctttca ccacttcgta catatcaacg tcaccgttca
     3721 ggtgcgccgc ttcgtggaag gttttcgggt tatcttcacg catggtggag cgcagatggg
     3781 tgaagtaaat acgcggaccg aactgcttga tcatatcaac caaatcgttg tcagcacgca
     3841 cgccgtagga accggtgcac atggtgaaac cgttcgccat gctgtttacg gtatcaacca
     3901 tccactgcat atcttcaatg gtggaaacaa tgcgcggcag gccgaggatc gggcgcggcg
     3961 gatcgtccgg gtgaacagcc atacggacgc caacttcttc ggcaaccgga ataatcgctt
     4021 tcaggaagac agcaaagttt tcgcgcagtt tggctttgtc gatatctttg tacagctcca
     4081 ggtgcttacg gaactggtcg agggtgtacc cttcttccgc acctggcaga ccagcgatga
     4141 tgttacgggt cagacgcgct ttgtcttcat cgctcatagt ggcgaagcgt tcagctgcct
     4201 gagcaatttc ttcttcggtg taatccgctt ccgcgcctgg acgtttcagg atatgcattt
     4261 cgaatgcagc gaattcgatc tggtcgaagc gcagagcttt ggagccgtct ggcagcacgt
     4321 attcgaggtc agtacgggtc cagtcgagca ccggcatgaa gttgtagcac acggtgcgaa
     4381 tgccgcactg cgccaggttg cgcagagtct gctgatagtt agcgatccac tgctcatagt
     4441 tgccagtgtg ggttttgata tcttcgtgaa ttggtacgct ttctacgaca gaccacacca
     4501 ggcctgcgtc ttcaacgatc gccttgcgtt tgaggatctc ttctacggac catacttcgc
     4561 cgttcgggat atggtgcagc gcggtaacca cgccagttgc gcccgcctga cggacatcag
     4621 ctaaagaaac cggatcgttt gggccgtacc agcgccaggt ctgttccatg atttttcctc
     4681 tttcacatag tgggttccac agcaaagaga cgcggaaccc tggagttgtt taggtatccg
     4741 gatgaacaac cagaccgcag ccgtggcgtt gcatcggatg agatggcgta taagttctac
     4801 cttttggtaa gacatgtcaa cccgaattct gaaattggtt aaccacatca caagaatttc
     4861 actctcatgg ttgttgctgc aaattgcagc aaagggtaat cgatggttat tgatcagagg
     4921 atgtgacatt catcgcaaca atggttgacc aatttacata acatgtcgcc caaaataaca
     4981 acggttcaac caggtaaggt aaaacgttat gcatgtgctt aacattctct gggtggtatt
     5041 cggcattggt ctgatgctgg tactgaattt gaagttcaaa atcaattcaa tggtggcttt
     5101 gttggtggcg gcgctgtccg tcgggatgct ggcgggcatg gatttgatgt cgctgctgca
     5161 caccatgaaa gcgggcttcg gcaacacgct gggggaactg gctatcatcg tggtgttcgg
     5221 tgcggtcatc ggtaaattga tggtcgactc cggcgcggct caccagatag cgcatacgct
     5281 gctggcgcgt ctcggtctgc gctatgtaca gctgtcggtg attatcatcg gcctgatttt
     5341 tggtctggcg atgttctatg aagtggcctt tatcatgtta gcgccgctgg ttattgtcat
     5401 tgccgccgaa gctaaaattc cgttcctgaa actggcgatc ccggcagtag cagctgccac
     5461 taccgcacat tcactgttcc caccgcagcc gggtccggtg gcgctggtga atgcttatgg
     5521 cgcagatatg gggatggttt atatctatgg cgtactggtg acgatcccaa gtgtaatctg
     5581 cgcaggtctg atcctgccga agttcctcgg caatcttgag cgcccaacgc catcattcct
     5641 gaaagcagat caaccggtag atatgaacaa tctgccctct ttcggcgttt cgattctggt
     5701 gccgctgatc ccggcgatca ttatgatctc taccaccatc gccaatatct ggctggtaaa
     5761 agatacccct gcctgggaag tggttaactt tatcggttcc tcgccgattg cgatgtttat
     5821 tgcgatggtg gttgcattcg tactctttgg caccgcgcgt ggtcatgaca tgcagtgggt
     5881 gatgaacgct tttgaaagcg cggtgaagag tattgcaatg gtgattctga tcatcggtgc
     5941 gggtggcgtg ctgaagcaaa ctatcatcga caccggcatt ggcgacacca tcggcatgtt
     6001 gatgtcccac ggcaatatct cgccctacat tatggcatgg ctgatcactg tgctaattcg
     6061 tctggcgacg ggtcagggtg tcgtttcggc gatgaccgcc gccgggatta tcagtgctgc
     6121 aatccttgat ccagcaactg gtcagctggt tggcgtgaat ccggcgctgc tggtgctggc
     6181 gacggctgcg ggttccaaca ccctcaccca cattaacgat gcatctttct ggttgttcaa
     6241 aggttacttt gacctgtcgg taaaagacac gttgaaaacc tggggtctgc tggagctggt
     6301 caactccgtg gttgggctga ttattgtgct gattattagc atggtagcgt aaaagaacac
     6361 cagagcctgc caatggcagg ctcagttcgg attgtcggca aagtgcgctt tgtttatgcc
     6421 agatgcgacg ctgacgcgtc ttatctggcc tacaaagggc taacgtgcag gtttttagct
     6481 tcaggtaata ttgcgtacct gcattagcaa tgccctgtga tttctttatt gataaacaaa
     6541 agtcacgcca ataatcgatt gcacattccc tgcagtcacc tgcccgccgg tacgtgcgta
     6601 atttgccgtt aatcccagac ttaccgccga agttcctact gctcctaacg ataccgtgtt
     6661 attcgctgga ataatcgtac cgttgcgcgt caactgtacg ccgacgccct gcgctggtga
     6721 aaacgacgcg gtattggtga aaatcgagtt gcccgcatct gcggttgtgc cggagaggta
     6781 atacccctgg ttttggcttt tcgcacaata aacggtaaga ggaattggca ctgaaccagg
     6841 gtagtccggc agagtaacgg tgacatcacg agcagaaaca tcgcagccgc cagtaggcac
     6901 taccacatca ttattggcgt aaatattcca cacaaactgg aaatcatcgc tgttatagtt
     6961 gttggtctgt cgcaaaataa gcacggcaat taatgagcca gctttaatcg ccaccccgcc
     7021 cgcactgctc acaggcgtca aataaagcgc caccggccac ggcttatccg ttctcgaatt
     7081 ataaacaacc cgcggcgttt cgctggtggt cggaaatgga tagctactgc cactatattt
     7141 tacggtcccg gaaaaattag ataacacgcc gccataagcc gagcctcgtt gcagtgtgac
     7201 atagtctgta atggtttccg gataatcgtt atggcaaaag atttgcgtcg aaagatctac
     7261 gaccaggttt tgccccacat tcacggcagg cgcaaggttt acataaacat tagcgctgcc
     7321 accgccaata gggatagcgg taccattggc ggttttacag gcgaatgacc aggcatttac
     7381 cgaccagccc atcagcagta cagcaaacag ggtaataact cgtttcatta caattatctc
     7441 ttcgggttca gctgtaggta taggtgatgc taatcactgc ctgaatggtt ccctgagtgg
     7501 ctccgccatt taccgtcaat gctctgacct gtaacgggaa gtgcgctgat tgtgaggaat
     7561 catccacctg aactgttttg gttgcgccag tattcaatgt gttgccactg tcatcctgta
     7621 gctctaactg gatgttttgc gcggtcccct ggtttttata atatccggta ctgtcggctg
     7681 ccccgctgaa gctggcagtg acccttgacg ttcccaccgg acaattagtc aactcaagcg
     7741 caacatcatg ccaggccgat gccgccccgg cagacatcag actgaaagaa taaagatcgc
     7801 cgagatcaac cgtggcattg gtggtggaaa ctgtgcacgg tttggcgacg accttaccgt
     7861 tcaccgtgat ggtgacatcg gctgcctgta tcgtcgcact tgcgagcgcc aatattgccg
     7921 ccaataaata cccacgtttg cgccatttca tgagcatctc cagttactga tattcaagag
     7981 tgaaggtagc cgtggcattg atatgccccg cagtgacagg cacctgtgtc gccattagcc
     8041 gggcgtaaaa attcaacgta tttggtttac ccggcgtcag ggtcgtccac gaaatcgcgg
     8101 acgatggagc attaaggggt atctgatttt gctgctcatt cagaagctgt attcccagtc
     8161 ccgaagccgc tgacaccgta ttttcaagtg caagcaggtt ggcattgtgg ctatctgcaa
     8221 cgccggtaaa cccaactttt acggcagaaa cggcgttacc acagggtgac agcaaaatac
     8281 gaaatggaac gacaggagtc gtcgcgccaa tgttgttaaa ttgcttcgcc gcgttttcca
     8341 tcagatcaac agtaaaattg gttgattcag cggccacact acagccgtta tctctgacat
     8401 agccgcggat agtaatcgtg ctatccgcag ccaaagcgtg actcactgcc agccacaaaa
     8461 aagcgcacag aagataaaaa ggtttgtttc tcatcacgcc cccttaacga cattcagctg
     8521 atagctgggt taataactgc tgctgactct ctggtggcag ttgataattg gcgacacaat
     8581 gagcattttc ctcttctccc catttcacct gaacttttcc cgctagaggc attccgctga
     8641 ggtaaacctg accattatcc gcaacaatgc cgctactctg gctactctct gatgtcacca
     8701 tcgccccaaa cggcagcggc ttattattgt gggttagcgt catgagcagt tttatcccaa
     8761 cgcgcgcttt aaactctgct cgcacgatcg ccccacgagt gggaacaacg ttagcgaccg
     8821 cgttatctaa atcgacgtta tcagccaggg tattggtatc cagcgccact ctattttccc
     8881 gatattcagt ggcataaggc agcacggcat aaccgcgcca gtcggtacgc acccccgtct
     8941 ggttttcgac ttttgcatct tttgcgccag gcgctttaac aagcaccacc gtatcgttta
     9001 acggctgccc cagcgttacg ccattggcat gagccagtac cccaccgctg actccgtaat
     9061 agagctgctt aatatcatcg ctatggctgt aaccgatatt ggcattgccg taaccaccgc
     9121 gataattcag cgtggcgtag cctgtgcttc cgctattacc atcgcctccc ccggcatagc
     9181 cggtttgcac gctatagctg aggttgttgt cttccagcaa cgtaccgtat acaccagcca
     9241 gattggtcat ccgaccgttg agatcgtgtg acatgctgta gctggcactg gcatgtcgcc
     9301 actgagattt actgtcagaa cgcagccagt ggctgaaagg aatattgacg ttacgcgcta
     9361 acatctgatc acgtcctttt tgccaggcgt ttttcgtcag gctatagctg agcgtccagt
     9421 tgatatcttc gaacgcagta tttaatccag cctggaattg ctcatcgaca ttactcgttc
     9481 cccaataagt ttgatggcta ccactcaaat acagtgttga tgagcgcccg agttgctgag
     9541 taacggtgag ttgtaatttc ccgcgtttgt tataagcgag gttgtaatag tcggtgaatt
     9601 tcggcttaac ctgaataact ccgtcctgtg tttcgatgtt gtagccattc attcgactgt
     9661 atgttgtatc agcgaaatta aaatatccgc tggtcgaata acggtaaccc actaactgaa
     9721 tattcgtgcc tgactcattg agcgatttgt tatagagaaa acgcaccgat tgtccgtcat
     9781 gctgactgtc atcgggaagt gtggaattag cctgagtcat atccacagac agagcgccca
     9841 gtgcccccat atttttcccg ataccaaaat taaaagcacg ataacgatct gccagttgcg
     9901 ttccaccata tattgtccag ccagctggaa ggccgtggag taatgtactt tggaaaaagc
     9961 ggggtttttc ctgttgcgca tttccactac ggtattctcc tgccgtaatg gaataacgag
    10021 tatgcccttc acgttgcaaa agcgggactg acgaataggg tacggtaaaa atctgcgtgc
    10081 tgccgtcagc ctctttaatc gttacctgca agtcaccact attacctgcg gcatagatat
    10141 cgttgatggt aaaaggcccc ggcggcaccg tactattata aatgtcatac ccattttgtt
    10201 taatagtgac ctgtgcagta ccacgagcaa taccgtggat caccggggca aatcctcttt
    10261 ggctatcggg taacatattg tcatctgagg ccaattgtgc gccgcgaaag ttaataccat
    10321 cgaaaatatc accctgagta taaccatcac ccagcgtcag ccgggaacgt aacggtatta
    10381 tgtctcgctc aagccaggta ttgatatgct gccatttatt tttgctacct gatgatctgt
    10441 cgctactgtt ataactccag gtggtattgt cgcgtaaacg ccacgcacca atatttaacc
    10501 cactctgtag gtttaaatat gcataatggc tgttaccccc aatccgattc tgtacactat
    10561 ttccgctgaa attataattg agcaatccgg cattaatacc gggatcccat aactcaggag
    10621 gaatataacc acgcgcgcga ttactcataa atgcctgagg gatcgtcagg ttcagtcgct
    10681 gctgaccaac atctaaatgc gcagtagcgt cctggaccat tgtggttaat ggcacacagg
    10741 catcatccgc cagcagattc ataccggcga cagaagccgt attcagcccc atactggcga
    10801 gttgcgcgcg tgtcaggcag ggaacaatcc cttgttcact gtcgcccgta ttaaatgtga
    10861 catcacgcgt tgccatataa ccattattca aatagatatc gacgcgatac gtccctggcg
    10921 gtaattcttg cccattttca aaacgcgata aatcggccac agcctgggga tcatccgcta
    10981 aaaagcgcgg attaaaatag agttcggcag atgacaaagg tgcctgtacg gcaaaagcac
    11041 aggcgacaaa gagccggaca aaaaaaccag ccaaacgatg cttacgaata tgcaagcatt
    11101 gtgtgtttcg ctggtaaagt cttaaattca gatatgacat tattcctccg gtttaaaatc
    11161 acccggcagt caattctttt tattcaggcg aaaaattccc cctgcgttat tccattacgc
    11221 ccgtcatttt gggggtaagt gcaccataat catttattgt tcggtaagta atattgcttc
    11281 ccgcatcaga aggcaattta accgtgcttt cgcccattgg aggcaccaag gcattttcaa
    11341 gaacccgggc tccggcattc aactctgtta ccgtcaggta atagggtgtc gggttaatca
    11401 gcgtcagaga attcgcgcta cgacgaaatc ttaatttttc tgcggcctga tcgggtggca
    11461 acgctaattt agccggacga tagtacagtt taatgcggct gataattgcg agctgtagcg
    11521 tattctcagt caattttgat ttatccattg acggaatcgc tttaacgttc atccagaata
    11581 aactttcccg gtcctgtggc aattggttat ttgttgcatc aagaatacgt aaggtattct
    11641 ctttttttcc cttcatcgca aacagaggag gcgtcacgat aaaacgacca tcctttacac
    11701 catcggcatt ttccacccat gattgaatta aataggtact attttcatca ttatttgtca
    11761 cggcaagttg cacttgtttt tgccctgccg gataaattat gcgagtcgca cctaaggcca
    11821 ctcccgcttc agcgcgtccg gcaaccatca ttgccatgaa catcaggata cctgccagca
    11881 agcagaatgt tatttcctgc gatttcctta cattgacgtt tttattactc actgtcctgt
    11941 tcctgttatc acattatctg ctgaacaatt actgataggt taaagagaac caggcctgtg
    12001 cattggcgat gccgccagta acccgacgcc cggtagcacg atatttggcg atgaaatgta
    12061 gcgaagtaga gcctgagtaa agccgtttcc agtttgctgg aggacgatta atcggtacga
    12121 ggtttccttc atcatcaaac aacgctacgc caatattggt ggctatccct ggcccctctc
    12181 ccacggaaag cacatccgga tttttaccat ccgcgacacc gtgaaacgcc acacccacac
    12241 gttcactcac caccgtgcta cattcccgta aatgaataac aaaaggcacc ggtgcgctat
    12301 cttcccctac cgcatgaaac cggttactgc tgatttgccc catattgacc gtcatttgtt
    12361 tatcaccggc ttcaatcagg caagtttccg caataatgac gccctgaaat tgcatatttc
    12421 cgccgggcgg cgtggtattc catttatttc cggccagagc aaacattggc agcaacgacg
    12481 ctaatagaaa taatctcttc cttttcatcg ttatttttat cgcacaaggg tgggcatccc
    12541 tgcccgtaat gacgtccctg aacctgggta ggttattgat actgaacctt gaaggtcgca
    12601 tccgcattag cagcacccgg ggttgcctcg ccgattgcat aataacgcgc ctggaacgga
    12661 atggtgttag taccgttatt cagggttgtt tgctcactga atgtcgcacc atccagcgtc
    12721 agcgcagcac ccgttctgtc caggatctgc acaccaacgt ttgttgcgct acccgcagct
    12781 gaactctgca gagccagaac gttggtatga cccgcatcaa tcgccgtacc taaaaaggca
    12841 acagcggctt tagatgcaac attggtatcg caatcattca gctgaatgtt aaaaccgaca
    12901 gcagaactgg ttgctccgtc ctgtgccagc gatgcggtac gaacctgtcc taactgaacg
    12961 gtttgatcaa cagagcctgc atcaactgcg caagcggcgt taacaacttc ccctttaaag
    13021 tgaacggtcc caccattaac cgtcgtggca gcggccagag ccgctgtaga actgagggac
    13081 agagccgaca gaacaacgat tgccagagtt ttaattttca tgctgctttc ctttcaaaaa
    13141 actatttcta aatcgacatg ggcagtcgtt ctgtacactt tgttttgtca acgagtttga
    13201 ctgccaacac tgcacagttt tcccccaaaa gatgaaacat ttggggccat tttgactcat
    13261 agaggaaagc atcgcgcaca aactttttca gtttatttgc tggattaata ttctattgtt
    13321 atctttattt atagattttt atattgcatg aggtggtttt tggagagaag aatgaggaag
    13381 atgcgtcgag cc
//
LOCUS       DAZKIP010000119        12716 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZKIP010000119 DAZKIP010000000
VERSION     DAZKIP010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.120"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(142..837)
                     /gene="aqpZ"
                     /locus_tag="ACHOUK_003753"
     CDS             complement(142..837)
                     /gene="aqpZ"
                     /locus_tag="ACHOUK_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFP9899114.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRN"
     gene            1263..2921
                     /gene="ybjD"
                     /locus_tag="ACHOUK_003754"
     CDS             1263..2921
                     /gene="ybjD"
                     /locus_tag="ACHOUK_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFP9899115.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQAHHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2918..3874)
                     /gene="ybjX"
                     /locus_tag="ACHOUK_003755"
     CDS             complement(2918..3874)
                     /gene="ybjX"
                     /locus_tag="ACHOUK_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFP9899116.1"
                     /translation="MNMSQLTERTFTSSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            4025..5140
                     /gene="macA"
                     /locus_tag="ACHOUK_003756"
     CDS             4025..5140
                     /gene="macA"
                     /locus_tag="ACHOUK_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFP9899117.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            5137..7083
                     /gene="macB"
                     /locus_tag="ACHOUK_003757"
     CDS             5137..7083
                     /gene="macB"
                     /locus_tag="ACHOUK_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFP9899118.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTASGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFMTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(7156..7380)
                     /gene="cspD"
                     /locus_tag="ACHOUK_003758"
     CDS             complement(7156..7380)
                     /gene="cspD"
                     /locus_tag="ACHOUK_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFP9899119.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            7530..7604
                     /gene="yljB"
                     /locus_tag="ACHOUK_003759"
     CDS             7530..7604
                     /gene="yljB"
                     /locus_tag="ACHOUK_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFP9899120.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            7703..8023
                     /gene="clpS"
                     /locus_tag="ACHOUK_003760"
     CDS             7703..8023
                     /gene="clpS"
                     /locus_tag="ACHOUK_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFP9899121.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            8054..10330
                     /gene="clpA"
                     /locus_tag="ACHOUK_003761"
     CDS             8054..10330
                     /gene="clpA"
                     /locus_tag="ACHOUK_003761"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFP9899122.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            10850..12052
                     /locus_tag="ACHOUK_003762"
     CDS             10850..12052
                     /locus_tag="ACHOUK_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9899123.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQIVNK"
     gene            complement(12239..>12716)
                     /locus_tag="ACHOUK_003763"
     CDS             complement(12239..>12716)
                     /locus_tag="ACHOUK_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899124.1"
                     /translation="DHEYPESCLQNDTNTYLLGRVIFLYNSMIYKFINCQEHETNNIH
                     LAMINNLLQEVDIAIGKINNIIDSRNISAPHELANILTREKILTTREKKGNLISLFDG
                     FTLFHCVGMITFLIHYLRTPEEKVENIFMLYGADKNNKLRRRLIYDALGIIQSQQE"
BASE COUNT         3245 a         2919 c         3395 g         3157 t
ORIGIN      
        1 gtaagacgcg ccctcagccc acatccatgt gggctgcccc ggccttcggg gaacgcttca
       61 gcgattttga cgccaccaat acccgtgcgg tttgtaatgt cgaagataaa acaaccaggg
      121 cagataaaat gccaatgcta attaattacg cttttccagc agagtccggt aaatcagacc
      181 accgataatg ccaccgacaa ttggcaccac ccagaagaac cacagttgtt ctaatgccca
      241 gccgccctgg aagatggcaa ccgcggtgct gcgcgccggg ttaacagaag tgttagtcac
      301 cggaatactg attaagtgaa tcagggttaa ggccagacca atagcgatcg gcgcaaaacc
      361 tgccggagcg aatttgtcgg ttgcaccgtg gatcactaac aggaagcccg cactcaatac
      421 cagttcaact accagcgcgg aaagcatgga ataaccgcct ggtgaatgct cgccataacc
      481 gttagaagca aaaccgctgg ctgccgcgtc aaaacccgtt ttaccactgg caattaaata
      541 cagcagcgct gccgcaacaa taccgccgac aacctgggca attacgtagc caacgacttc
      601 ttttgccgga aaacgtccgc cagcccataa accaatagtg accgccgggt taaaatgacc
      661 accagaaata tgaccaacag caaaggccat cgtcagaacg gtcagaccga acgccaacgc
      721 cacgccggca aaaccaatgc ctaattccgg gaagcctgcg gccagtacag cactaccaca
      781 gccaccaaaa acaagccaga aagtaccaaa acattcagct gctaattttc tgaacatatc
      841 cacctcaatt aaaaattgac cctgtgaaaa atatggtcgt tttatagggt cgtcgtaaaa
      901 aagtgacgac ggaaataatg cgcggctatt ttaaaaacga aggcgagtca ttcaccagat
      961 aaataaatcc agtaaatttg atttagggca acagcgggtt gccccatata gtcatttgtc
     1021 tgattgacag tgtagtgcaa gcaaaagatt taatccttta ggcgtaataa aaaataattt
     1081 atcatgctaa ttatttgatt ttgttgtttt tgcagactta tcagcaagag ggagtataac
     1141 gcgattattc gctcattttt cagacatttg ccatgcttaa atgtgatgtc atcacgtatt
     1201 agcaaggcct ttcccgttat actgccagcg taaaggataa gtcacatatt tctggagggg
     1261 atatgattct tgagcgcgtt gaaattgtgg gttttcgcgg tatcaaccgt ttgtcgttga
     1321 tgctggaaca aaacaacgtc ctgattgggg agaacgcgtg gggtaaatcc agcttgctgg
     1381 acgccttaac cctgctgcta tcgccagaat cagatctcta ccattttgag cgcgacgatt
     1441 tctggttccc gccgggagat atcaacgggc gagaacatca tctgcatatt attttgacct
     1501 tccgcgaatc gctgccaggt cgacatcggg ttcgccgtta tcggccgctg gaagcgtgct
     1561 ggacgccatg caccgatggc tatcaccgta ttttttatcg tctggaaggg gagagtgcgg
     1621 aagacggcag cgtgatgaca ctgcgcagtt ttctcgataa agacggacat ccgattgatg
     1681 ttgaggatat taacgatcag gcacaccatc tggtgcgttt aatgccggtg ctgcgcttgc
     1741 gtgatgcccg ttttatgcgc cgtattcgta acggcacggt gccaaatgtc cccaatgtgg
     1801 aagtcaccgc gcgccagctc gatttcctcg cccgtgagtt gtcctcacat ccgcaaaatc
     1861 tctctgatgg acagattcgt cagggacttt ccgcaatggt gcagttgctt gagcattatt
     1921 tctctgagca gggggctgga caggcgcgat atcgtttaat gcggcggcgg gccagcaatg
     1981 agcaacgaag ctggcgctat ctggacatca tcaaccggat gattgaccga cctggagggc
     2041 gctcgtatcg ggttattttg ctcggcctgt ttgctacttt attgcaggca aaaggcacat
     2101 tgcgactgga taaagacgcc cgtccattgt tgctgatcga agatccagaa acgcgtttac
     2161 accccattat gctttcagtt gcctggcatc tgttgaatct tttgccttta caacgtattg
     2221 ctaccaccaa ctctggcgag ctgctttcgt taactccagt ggaacatgtt tgccgcctgg
     2281 tgcgtgaatc ttctcgtgtt gccgcctggc gtctggggcc aagtggcttg agcaccgaag
     2341 acagccgccg catctctttt cacattcgtt ttaatcgtcc gtcatcgctg tttgctcgct
     2401 gctggttgct ggtggaaggg gaaacggaaa cctgggttat caatgaactg gcgcgtcagt
     2461 gcggacatca ttttgatgcc gaagggatca aggtcattga gtttgcccag tccgggctaa
     2521 agccactggt taaatttgcc cgccgaatgg ggattgaatg gcatgtactg gtcgatggcg
     2581 atgaagcagg gaagaaatat gccgctacgg tacgcagcct gttgaataat gatcgggaag
     2641 ccgaacgaga acatttaacg gcgttaccgg cgctggatat ggaacatttt atgtatcgcc
     2701 agggattttc cgatgtgttc caccgcgtgg cgcaaatccc ggaaaatgtc ccgatgaatc
     2761 tgcgcaaaat tatctcgaaa gcgatccatc gctcttccaa acccgatctt gccattgaag
     2821 tggcaatgga ggccggacgt cgtggtgtgg actccgtacc gacgctgctg aaaaaaatgt
     2881 tctcacgcgt gctgtggctg gcgcgcggtc gcgcggatta accgcgaaac atcgtggcca
     2941 tttgtggctg aatagcgtcg agcatctcat agcgccgacg gtattcagcc cgttttttac
     3001 tggcgatttc ggcaatctct tttcgtgcta tctgtgctgg aaggcggtaa tggcgttcag
     3061 catcacatac gccgccaacc gattcccaga aagcgttgta atcggcatgg atcttgcctt
     3121 ctttatcgcg ataacgcagg ctgcggtaaa tatgcgtttc attgctgacg gcaataatct
     3181 gctctacctg caaacgttgg gcaaacagac aggccgcttc catcacgagg cgtttgggaa
     3241 atagcccgtg gcaggctttc gtcgcattct ggatttcctg atgtggaatt tcccattttg
     3301 cgccttgcag tccaccaata aacatcgttc ttttcccctg atattcacac agggtaaacg
     3361 tgatttctgc cagaggaata ccttcgctgt tgcggaacag gattgtgctg tcaccttctt
     3421 tatccattga gatcatcatg gtcagctcaa gcgtgaactg ctcgccgttt ttgccttcca
     3481 gcttcgccag ttgcagcccg ggggtattca aatataagct gaattcttcc gccgacatac
     3541 atccacggag taacgcataa tggtaacgta acgcctccag caattgctta cggctaagat
     3601 tcgccgcaag gtaagggcga tgcagacgca caggcagtcg cggctggcgc gttaacaata
     3661 cattgagatt aggccagtgg gaaagttcgt tcatccactc aacgcttaaa cgcggcataa
     3721 tcaacgagcg cagcaaaaat ttctggcgaa aactacggcg atgccagaat ttacccggcc
     3781 gacactgtcc acgtgccaga ctaagaaaaa gtgacaggct gctgagagat tcagatgacg
     3841 taaaggtccg ttcggttagc tgcgacatat tcatgaaatc aatggttgta catgacgtcg
     3901 atttcaccat tgcgtatctt aaccaaacat caatagtgtg attactaacg taaattttag
     3961 ggttttgttg atatttcgtt gaagttaatg acccggattg gcatatggag tattcagaat
     4021 atttatgaaa aagcggaaaa ccgtgaagaa gcgttacgtt attgcgctgg tgatagtcat
     4081 cgccggactg attacgttat ggagaattct taacgcaccc gtgccgactt atcagacact
     4141 gattgtgcgc cccggtgatt tacagcaaag cgtgctggcg accggaaagc tggacgcgct
     4201 gcgtaaggtt gacgtgggcg cgcaggtcag cggtcagttg aaaacgctgt cggtggcgat
     4261 tggcgataaa gtaaaaaaag accagctttt aggggttatt gatcctgaac aggctgaaaa
     4321 ccagatcaag gaggtcgaag caacgctgat ggagctacgc gcgcagcggc agcaggcgga
     4381 agcggagctg aaactggcgc gggtgacgta ttcccgtcag caacgtctgg cacaaacgca
     4441 ggcggtttca ctgcaggatc tcgacaccgc cgcgacggag atggctgtga aacaggcgca
     4501 aattggcacc attgacgcgc aaatcaagcg caatcaggct tctctcgata cggctaaaac
     4561 caatctcgat tacactcgca tcgttgcccc gatggccggg gaagtcacgc aaatcaccac
     4621 tctgcaaggc cagacggtga ttgccgcaca acaagcaccg aacattctga cgctggcaga
     4681 tatgagcacc atgctggtaa aagcgcaggt ttctgaagcg gatgtaatcc acctgaagcc
     4741 ggggcaaaaa gcctggttta cggtacttgg cgatccactg acgcgctacg aggggcaaat
     4801 caaggatgta ctaccgacgc cggaaaaggt taacgacgct attttctatt acgcccgttt
     4861 tgaagtcccc aaccccaatg gtttgctgcg gctggatatg actgcgcaag tgcatattca
     4921 gctcaccgat gtgaaaaatg tgctgacgat ccctctgtcg gcgttaggcg atccggttgg
     4981 cgataatcgt tataaagtca aattgttgcg taatggtgaa acacgcgagc gtgaagtgac
     5041 gattggcgca cgtaacgata ccgatgttga gattgtcaaa gggctggaag cgggcgatga
     5101 agtggtgatt ggtgaggcca aaccaggagc tgcacaatga cgcctttgct cgaattaaag
     5161 gatattcgtc gcagctatcc tgccggtgat gagcaggttg aggtgctgaa gggcatcacc
     5221 ctcgatattt atgccggtga gatggtagcg attgttggcg cttcgggttc cggtaaatcg
     5281 accctgatga atattctcgg ctgtctggat aaggccacca gcggcaccta tcgcgtcgcc
     5341 ggtcaggatg ttgccacgct ggacgccgat gcgctggcgc aactgcgccg cgagcatttc
     5401 ggctttattt tccagcgtta ccatttgctt tcgcatttaa ccgccgagca gaacgttgaa
     5461 gtaccagccg tctatgctgg tcttgagcgg aaacagcggc tgcttcgcgc ccaggagttg
     5521 ctgcaacgac tggggctgga agaccgtaca gagtattatc cggcacagct ttcgggtggt
     5581 cagcaacagc gcgttagcat cgcgcgggca ttgatgaacg gcggtcaggt aattcttgcc
     5641 gatgaaccga ccggcgcact ggacagccat tcaggcgaag aggtgatggc gatcctgcat
     5701 cagctgcgcg atcgcgggca tacggtgatt atcgtcactc acgatccgca ggtcgctgct
     5761 caggccgagc gggtgatcga aattcgcgac ggcgaaattg tgcgcaatcc tcccgccatt
     5821 gaaaaagtga atgttgctgg cgggacggaa cctgttgtca acacggcgtc tggctggcgg
     5881 cagtttgtca gcggttttaa tgaggcgctg acgatggcat ggcgggcgct ggcagcgaat
     5941 aaaatgcgta ctttactgac catgctgggg attataatcg gtattgcgtc ggtggtttcc
     6001 attgtcgtgg tgggcgacgc cgccaaacaa atggtgctgg cggatattcg ttctattggt
     6061 acgaatacta ttgatgtcta tcccgggaaa gattttggcg atgacgatcc gcaatatcaa
     6121 caggcgctga agtacgatga cttaatcgcc atccaaaaac aaccgtgggt cgcttcagcc
     6181 acacctgccg tctcgcaaaa cctgcgcctg cgttataaca atgttgatgt tgctgccagt
     6241 gccaatggcg tgagcggcga ttattttaat gtctatggca tgaccttcag tgaaggaaac
     6301 acctttaacc aggaacagct gaacggtcgt gcgcaggtcg tggttctcga cagtaatact
     6361 cgccgccagc ttttccccca taaagcagat gtggttggcg aggtgattct ggtcggcaat
     6421 atgcccgcca gagtcattgg tgtggcggaa gaaaaacagt cgatgtttgg tagcagtaaa
     6481 gtgctgcgtg tctggctacc ttacagcacg atgtccgggc gagttatggg ccagtcgtgg
     6541 ctgaactcca ttactgtcag ggtgaaagaa ggatttgaca gcgccgaggc ggaacagcaa
     6601 ctcacgcgtt tactttcact gcgccacggt aagaaggatt tctttacctg gaacatggac
     6661 ggcgtcttga aaaccgttga aaagaccaca cgtactttac aactgtttat gacgctggtg
     6721 gcggtgattt cgctggtagt gggcggtatt ggtgtaatga atattatgct ggtgtcagtg
     6781 accgagcgga cgcgggaaat tggcattcgc atggctgtag gtgcgcgagc aagcgatgtt
     6841 ttgcaacagt tcctgatcga agccgtactg gtttgcctgg tcggtggcgc gttgggaata
     6901 acactgtcac tgttaattgc tttcaccttg cagcttttct tacccggctg ggagattggt
     6961 ttttcaccgt tggcactgct gctggcgttt ctctgctcga cggtcaccgg gattttattt
     7021 ggctggttac ccgcacgaaa tgcggcacga ctggatccag tagatgctct ggcacgagag
     7081 taatttttga gataaaaatg ccagccgatc gggctggcat tttgccttta ggatgtacac
     7141 aatgagacag aagagctatg cgactgccgc ttctacttcg acgggcacaa taacactggc
     7201 gtgattgcct tttggcccct ggtggacatc aaactgaacg gattgtccag cttttagcgt
     7261 tctgtaacca tccatctgaa tggtggaata atgagcgaaa atatcttcgc cgccgccttc
     7321 agggcagatg aaaccaaacc ctttggcatt gttgaaccac ttaacagtac ccttttccat
     7381 gcttcgacat ccttcgcaaa tcttatacaa gtaagatgga ataaaccggg gtcagagagg
     7441 gggctgttca aaacctcgcc aactctagaa atacaattta gagaattagg gcgagccgtc
     7501 aagcatttga caggggacag ggggcaggta tgaatcaaaa atttgaagca gttaacgcta
     7561 ttgacaggaa tgtgacagat gtcgctgatg ccaacgatag atgatagtta tctatcatgt
     7621 ggagtagatt ggtcaggcaa ataagctctt gtcagcggca gggcgttctg ccgataaccg
     7681 taaccgaaga tgataactga caatgggtaa aacgaacgac tggctggact ttgatcaact
     7741 ggcggaagaa aaagttcgcg acgcgctaaa accgccatct atgtataaag tgatattagt
     7801 caatgatgat tacactccga tggagtttgt tattgacgtg ttacaaaaat tcttttctta
     7861 tgatgtagaa cgtgcaacgc aattgatgct cgctgttcac taccagggga aggccatttg
     7921 cggagtcttt accgccgagg ttgcagaaac caaagtggcg atggtgaaca agtacgcgag
     7981 ggagaatgag catccattgc tgtgtacgct agaaaaagcc tgaatgcagg cataaaaatt
     8041 gggggaggtg cctatgctca atcaagaact ggaactcagt ttaaatatgg ctttcgccag
     8101 agcgcgcgag caccgtcatg agtttatgac cgtcgagcac ttgttactgg cgctgctcag
     8161 taacccatct gcccgggagg cgctggaagc gtgttctgtg gatttggttg cgctccgtca
     8221 ggaactggaa gcctttattg aacaaaccac acccgttctg cctgccagtg aagaggagcg
     8281 cgacacacag ccgacgctga gttttcagcg tgtactgcaa cgtgcggtct tccatgtcca
     8341 gtcctccggt cgcaatgagg ttaccggtgc aaacgttctg gtcgctatct ttagcgaaca
     8401 ggagtcgcag gcggcatatc tgttgcgtaa acatgaagtc agccgtctcg atgtggtgaa
     8461 ctttatctct catggcacgc gtaaagacga gccgacacag tcttctgatc ctggcagcca
     8521 gccaaacagc gaagaacaag ctggtgggga ggaacgtatg gagaatttca cgacgaacct
     8581 gaatcagctt gcgcgcgtgg gcggaatcga cccactgatt ggtcgtgaga aggagctgga
     8641 gcgtgctatt caggttctct gccgtcgccg taaaaacaac ccgctgctag tgggggaatc
     8701 tggtgtcggt aaaaccgcga ttgcagaagg tcttgcctgg cgaattgttc agggcgatgt
     8761 gccggaagtg atggctgact gtacgattta ctctctcgat atcggttctc tgttagcggg
     8821 cacaaaatat cgcggcgact ttgaaaaacg ttttaaagcg ttgctcaagc agctggagca
     8881 ggacactaac agcatcctgt ttattgatga gatccacacc attatcggtg cgggtgcagc
     8941 gtctggtggt caggttgatg cggctaacct aatcaaaccg ttgctctcca gcggtaaaat
     9001 tcgcgtaatt ggttcgacaa cctatcagga gttcagcaac attttcgaga aagaccgtgc
     9061 tctggcgcgt cgcttccaga aaattgatat tactgaaccg tcgatcgaag aaactgttca
     9121 aatcatcaat ggcctgaaac cgaagtatga agcacaccac gacgtgcgtt ataccgcaaa
     9181 agcggtgcgt gcggcggtag agctggcggt gaaatacatt aacgatcgtc atctgccgga
     9241 taaagccatt gatgttatcg acgaagcggg cgctcgcgca cgtctgatgc cggtaagcaa
     9301 acgcaagaaa accgttaatg tggcggatat tgagtccgtg gtggcccgta ttgcacgcat
     9361 tccagagaag agtgtttctc agagtgaccg cgataccctg aaaaacctcg gcgatcgcct
     9421 gaaaatgctg gtcttcggtc aggataaagc cattgaggcg ctgactgaag ccattaagat
     9481 ggcgcgtgca ggtttaggtc acgaacataa accggtcggt tcgttcctgt ttgccggccc
     9541 taccggggtc gggaaaacag aggtgacggt acagctttcg aaagctttgg gcattgagct
     9601 tctgcgcttt gatatgtccg agtatatgga acgccatacc gtcagccgtc ttattggtgc
     9661 gcctccggga tacgttggtt ttgatcaggg cggtttgctg actgatgcgg tcatcaagca
     9721 tccacatgcg gtgctgctgc tggacgaaat cgagaaagcg cacccggacg tgttcaatat
     9781 tctgttgcag gtgatggaca acggtacgct gaccgataac aacggacgca aagcggactt
     9841 ccgtaacgtg gtactggtga tgaccactaa cgccggggta cgggaaactg agcgcaaatc
     9901 cattggtctt atccaccagg ataacagcac cgatgcgatg gaagagatca agaagatctt
     9961 tacaccggaa ttccgtaacc gtctcgacaa cattatctgg ttcgatcatc tgtcaactga
    10021 cgtgatccat caggtggtgg ataaattcat cgtcgagttg caggttcagc tggatcagaa
    10081 aggtgtttct ctggaagtga gccaggaagc gcgtaactgg ctggccgaga aaggttacga
    10141 ccgggcaatg ggcgcacgtc cgatggcgcg tgtcatccag gacaacctga aaaaaccgct
    10201 cgccaacgaa ctgctgtttg gttcgctggt ggacggcggt caggtcaccg tcgcgctgga
    10261 taaagagaaa aatgagctga cttacggatt ccagagtgca caaaagcaca aggcggaagc
    10321 agcgcattaa tctgattgtc aggtaggttg gtgaagtccg taatctcgaa agaggttacg
    10381 gactttttat ttatggggtg gaacggtgaa aaccctattt ttggaggtga aggtaatttg
    10441 ttgataatta gtgctgctgg aaaaggtaag gataaaaaag ggtgacagca ggaaaatgag
    10501 atggttttgc tttattaaca acgggctaaa cgtgtagtat ttgagttcac tgccgtacag
    10561 gcagcttaga aattcactgg taacatactc cacccgccca ccatgttcac tgccgtacag
    10621 gcagataaaa tgcgaaaaaa aagcccgtac tttcgtacga gctcttcttt aaatatggcg
    10681 gtgaggggga ccgcctgaaa taaatctgac ttaatccagg ggggttatat aatttcatta
    10741 taaacaatgc attacacttt ttgttgtccg atattatcta atgtattctt cgtgaagcga
    10801 gagactaatg gcatactgga tggcatacca gagtgacggg aattagcgta tggcagtatt
    10861 gacggatacg aaagcaagac atatcaaacc tgatgacaaa ccattgcccc atgggggaat
    10921 taccggactg acccttcatc cttcttcagt aaaggggcgg gggaaatggg tttttcgtta
    10981 tgtaagtccg gtgacacaaa aaagacgtaa tgctggattg ggaacttacc cagaggtcag
    11041 tattgctgaa gctgcacgta ctgcccggat aatgcgagag caacttgctg caggtgatga
    11101 tcctctggag attaaaaagg ctgaatctga gaaagtcgtt atcccaacat ttgccgatgc
    11161 agccaggcgt gtacatgcag aactgtctcc tggatgggaa aatccaaagc atgtaaggca
    11221 gtggttatcg acgcttgaga attacgcgtt tcctcaactg ggagcaaaaa cgctggattc
    11281 gattacggct gcggacgtgg cagaaacact gcgtccagtc tggttaacct tgtcagaaac
    11341 ggcaagccgg gttaaacagc gcattcatgt tgttatgcag tggggatggg cgcacggttt
    11401 ttgtgtggca aatcctgttg atgtggttga ccatttgctt cctcagcaga caagaggacg
    11461 tgatgaacac caacccgcaa tgccctggag gcagttaccg ctttttgtgg cgaccagtgt
    11521 gtatagtgat gaaccttata atgttacccg cgcactgtta ttaatggtga tacttacagc
    11581 aactcgctcg ggcgaagcca ggggaatgcg ctgggctgaa attgattttc ataagcgggt
    11641 atggactata cccgcagaaa gaatgaaagc caggctacag catcgtgttc ctttatcccg
    11701 gcaggctatt tacattctgg aaaatatacg tggcctgcat gatgaactgg tgttcccttc
    11761 acccagaaag cagcagatcc tttccgatat ggtgttgaca agttttctgc gtaaaaagaa
    11821 agccgtcagt gacattccgg ggcgagttgc cacggcacat ggttttcgct caacattcag
    11881 ggactggtgt agcgaacagg ggtattcgcg ggatctggcg gaaagggcgc tcgctcatac
    11941 gctgaaaaat aaggttgagg cggcatatca tcgtactgat ctactggagc agcgtgtacc
    12001 gatgatgcag gcatgggcgg attatgtgat gtctcaaatt gtgaataaat aatagtgaaa
    12061 atgtatattg tacgatcatg atttgatatg tgatcgtggc agtagtaatt aagaaaccgc
    12121 ttttatgtca aatgtattga gctgataatt attagatcgt caggaaagcg tattttgatt
    12181 aacaggggat tacttaacaa tccccttttt ataaaaaata atttgcctgt ttaactcttc
    12241 actcctgctg agactgaatt attcctagtg cgtcataaat cagtcttctg cgtagtttat
    12301 tgtttttatc tgcaccatat aacataaata tattttcaac tttttcttca ggtgttctga
    12361 gataatggat aagaaaggtt atcattccaa cacaatggaa taaagtgaat ccatcaaaca
    12421 ggcttatcag gtttcctttt ttttcccgtg ttgtaagtat tttttcgcgg gtaagaatat
    12481 ttgccagttc atggggggct gatatgtttc tgctgtctat aatgttattt attttaccaa
    12541 tggctatatc aacttcctgt aataggttat ttatcatagc taagtgaatg ttattggttt
    12601 catgttcctg acagtttatg aacttataaa tcattgagtt atacagaaat attactcttc
    12661 ctaataaata tgtgtttgtg tcattttgaa gacaggactc tgggtattcg tgatcc
//
LOCUS       DAZKIP010000120        12391 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZKIP010000120 DAZKIP010000000
VERSION     DAZKIP010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.121"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            44..727
                     /locus_tag="ACHOUK_003764"
     CDS             44..727
                     /locus_tag="ACHOUK_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899125.1"
                     /translation="MKLPVKLLMSLISLVRVIARAGEYKNYSRDEIKYWRYTSYKGGK
                     LPEGFTDEKFSSAIYNGRIFTMKRLHTLMLFLAVLFTGFNFNAEAATVKQALSCNPEA
                     WAEQPGACPSTYELYEGDATYKAAIDKALKPVGLSGMFGKGGYMDGPGGGITPVNING
                     TVWFQGDGCKANTCGWDFIVTLYNPKTHEVVGYRYFGLDDPAYLVWFGEIGVHEFAYL
                     VKNYVAAVN"
     gene            743..1153
                     /locus_tag="ACHOUK_003765"
     CDS             743..1153
                     /locus_tag="ACHOUK_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899126.1"
                     /translation="MKTFIKTLLVAVTILFSVFATAKQVKLPNNIKYVNTTEAFSCTE
                     IDGMNCQTKNQFNYKDNSYVFVLERGGAWCYDYTVSVVNLKTGKAQMIEYGDNQLCSG
                     SNKPFFEIKNGVPTVGVIDTSGKPVGVAQDKLKI"
     gene            1374..2195
                     /locus_tag="ACHOUK_003766"
     CDS             1374..2195
                     /locus_tag="ACHOUK_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFP9899127.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELIRHVPSIFGEDRHTSRSER
                     YAYIPTITVLENLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPQPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETMLESLR"
     gene            2277..2756
                     /locus_tag="ACHOUK_003767"
     CDS             2277..2756
                     /locus_tag="ACHOUK_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFP9899128.1"
                     /translation="MKTVSQNTPTIYSATTPENNPPQLVASLVPDEQRISFWPQHFGL
                     IPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMN
                     GNRAEMSPEAAGIAACLMTYSHHACRTENYAMTVHYYRLRDYALQHPEGSAIMRIID"
     gene            2771..3247
                     /gene="yeeS"
                     /locus_tag="ACHOUK_003768"
     CDS             2771..3247
                     /gene="yeeS"
                     /locus_tag="ACHOUK_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFP9899129.1"
                     /translation="MQQISFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            3310..3531
                     /gene="yeeT"
                     /locus_tag="ACHOUK_003769"
     CDS             3310..3531
                     /gene="yeeT"
                     /locus_tag="ACHOUK_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFP9899130.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            3605..3973
                     /gene="cbeA"
                     /locus_tag="ACHOUK_003770"
     CDS             3605..3973
                     /gene="cbeA"
                     /locus_tag="ACHOUK_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system cytoskeleton
                     bundling-enhancing antitoxin CbeA"
                     /protein_id="HFP9899131.1"
                     /translation="MSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHPDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLSSCGYVYLAVYPTPEMKN"
     gene            4062..4435
                     /gene="cbtA"
                     /locus_tag="ACHOUK_003771"
                     /pseudo
     CDS             4062..4435
                     /gene="cbtA"
                     /locus_tag="ACHOUK_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system
                     cytoskeleton-binding toxin CbtA"
     gene            4432..4626
                     /gene="yeeW"
                     /locus_tag="ACHOUK_003772"
     CDS             4432..4626
                     /gene="yeeW"
                     /locus_tag="ACHOUK_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFP9899132.1"
                     /translation="MMTLEADSVNVQALDMGHIVVDIDGVNITELINKAAENGYSLRV
                     VDDRDSTETPATYASPHQLL"
     gene            4672..4752
                     /locus_tag="ACHOUK_003773"
                     /pseudo
     CDS             4672..4752
                     /locus_tag="ACHOUK_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5041..5185)
                     /locus_tag="ACHOUK_003774"
                     /pseudo
     CDS             complement(5041..5185)
                     /locus_tag="ACHOUK_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            5239..5421
                     /locus_tag="ACHOUK_003775"
     CDS             5239..5421
                     /locus_tag="ACHOUK_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFP9899133.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKCWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCR"
     gene            complement(5522..5851)
                     /gene="tmaR"
                     /locus_tag="ACHOUK_003776"
     CDS             complement(5522..5851)
                     /gene="tmaR"
                     /locus_tag="ACHOUK_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFP9899134.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(6023..7081)
                     /gene="yeeA"
                     /locus_tag="ACHOUK_003777"
     CDS             complement(6023..7081)
                     /gene="yeeA"
                     /locus_tag="ACHOUK_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP9899135.1"
                     /translation="MRANKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     GTWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSSLICRALRK"
     gene            complement(7280..7753)
                     /gene="sbmC"
                     /locus_tag="ACHOUK_003778"
     CDS             complement(7280..7753)
                     /gene="sbmC"
                     /locus_tag="ACHOUK_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFP9899136.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(7872..9038)
                     /gene="dacD"
                     /locus_tag="ACHOUK_003779"
     CDS             complement(7872..9038)
                     /gene="dacD"
                     /locus_tag="ACHOUK_003779"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFP9899137.1"
                     /translation="MKRRLIIAASLFIFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIALGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            complement(9878..10035)
                     /locus_tag="ACHOUK_003780"
                     /pseudo
     CDS             complement(9878..10035)
                     /locus_tag="ACHOUK_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701877.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
     gene            10363..11013
                     /gene="nleG7"
                     /locus_tag="ACHOUK_003781"
     CDS             10363..11013
                     /gene="nleG7"
                     /locus_tag="ACHOUK_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleG7"
                     /protein_id="HFP9899138.1"
                     /translation="MPIFLNFTAGSILPENELASLRYIVQQNQNDTVIIKERYKMDIR
                     YIESVNGFTVNPVCSNHFSIFMARQNTIARNLEQQINNGRSFAQISQDFMLQLSSNIG
                     WKKGAENALKNKIHSHSFVVNPDEFSCDTQFLKCPITLCVPEKGVFVKNALNSNICTL
                     YDKSAFMNLTREHLPHPLSREKIVKEMIIERNMCYFDTISQHFIIMDTDQQKQHCK"
     gene            complement(11238..12113)
                     /locus_tag="ACHOUK_003782"
     CDS             complement(11238..12113)
                     /locus_tag="ACHOUK_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspS"
                     /protein_id="HFP9899139.1"
                     /translation="MFSIRTLLPISASVSVPTKQSQSIPITLAGRTIEKAQEKEGLLV
                     FLGMKSVNDYTLNILGQNVSRVTTGKKPYDLLFLNDATKQDFDKRKMEFTYPGANKSH
                     LQSSNSDVVAAAAISITATEMKTILPDDLTLGKYNKIYLSGHGSAGLPLLKCGDEFLS
                     PSDIVDRIVQHNLHEIDDIRLTSCNSANIIKNKDFSPDEIEKSANMNNGWLARALFGQ
                     KRSLAEHVYAEFERRGINVSISGYHGTGVFYVPEHGKPTTHLRSTTVPATPEHTVRRS
                     DYRATLGRTQPIDIE"
     gene            complement(12254..>12391)
                     /locus_tag="ACHOUK_003783"
     CDS             complement(12254..>12391)
                     /locus_tag="ACHOUK_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFP9899140.1"
                     /translation="IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGRWENIGGM
                     K"
BASE COUNT         3336 a         3050 c         2772 g         3233 t
ORIGIN      
        1 gttttacccc tcacctgtca attcactcac tggaaattta attatgaaat tacctgtaaa
       61 gttattaatg agccttatat ctctggtccg cgttattgca cgtgccgggg aatataaaaa
      121 ttacagccgg gatgaaataa aatactggcg atatacatca tacaaggggg ggaaacttcc
      181 ggaaggtttc actgatgaga aattttccag cgccatttac aacggaagaa tatttacaat
      241 gaaacgttta cataccctta tgttatttct ggcggttctg ttcacgggct ttaactttaa
      301 cgcagaagcg gctaccgtaa aacaagctct tagttgtaac ccggaagcct gggctgaaca
      361 acctggagcg tgtccatcaa cgtacgagtt gtacgaaggt gacgctacct acaaagccgc
      421 tatcgacaaa gcattaaaac cggtcggact gagcggcatg ttcggtaaag gcggctatat
      481 ggatggccct ggtggaggta tcacgccagt aaacattaac ggtacagtct ggttccaggg
      541 cgacggttgc aaagccaata cctgcggctg ggactttatc gtaacactct ataacccaaa
      601 aacccatgaa gttgttggct accgttactt tggtttagat gacccggcct acctggtttg
      661 gttcggtgaa attggcgtgc atgaattcgc gtatctggtg aaaaactacg tagctgcggt
      721 taactaattt aaggagtcta tcatgaaaac ttttatcaaa actttactcg ttgctgtaac
      781 tattctgttc tctgtcttcg ctactgcgaa acaagtaaaa ctgccaaata acatcaaata
      841 cgttaatact acagaagcgt tttcctgtac cgaaattgac ggtatgaatt gccagacaaa
      901 gaatcagttc aactataaag ataacagcta tgttttcgtg cttgaacgtg gtggtgcctg
      961 gtgctacgac tacactgtct cggtagttaa cctgaaaacc gggaaagcac aaatgattga
     1021 atacggggac aatcaactgt gctcaggtag caacaaaccg ttcttcgaaa tcaaaaatgg
     1081 cgtaccgacg gtaggagtca tcgacacatc cggaaaacct gtcggtgtag cccaggacaa
     1141 acttaaaatc taaggagtaa accatgcgtt tctctgcaca ttattaatat caaaggtaaa
     1201 tacagcccca gccattgtta ccggaaagcc gcttaatgcg tggctcgcca gtgaaaggcc
     1261 tctcagacaa taaaccttat tcatttcacc cgtcaggccg cgtcttctcc gggagacgcg
     1321 gcttttttca tttattgcac cagtaaatct taaccaccga taaggagcaa agtatgcgat
     1381 tagccagtcg ttttggttat gctgcaaacc agatacgccg tgaccgtccg ctgacacatg
     1441 aagaactgat acgccatgta cccagtattt ttggggaaga ccggcacacc tcccgcagtg
     1501 aacggtatgc gtacattccc accattaccg tcctggaaaa tctgcagcgg gaaggctttc
     1561 agccgttctt cgcctgccag acccgtgtgc gcgacccggg ccgccgggga tacacaaaac
     1621 acatgctgcg tctgcggcgg gccggagaga taaacggaga acatgtccct gaaattattc
     1681 tgctcaactc tcatgacggt acctccagct accagatgct gccgggttac ttcaggttcg
     1741 tctgccagaa cgggtgcgtc tgtggccagt ctctggggga agtgcgtgtt ccacaccggg
     1801 gaaatgtagt ggagaaagtt atcgaagggg cttacgaggt ggtgggcgtg tttgaccgga
     1861 tagaggagaa gcgtgatgcc atgcagtcgc tggtcctgcc gcaaccggca cgccaggcgc
     1921 tggcacaggc ggcactgact taccgttatg gtgacgaaca tcagcccgtc accaccgccg
     1981 acattctgac gccgcgacgc cgggaggatt acggtaagga cctgtggagc gcatatcaga
     2041 ccatccagga gaatatgctg aaaggcggga tttccggtcg cagtgcaaaa ggaaaacgta
     2101 tccatacccg ggccattcac agcattgata ccgacattaa gctcaatcgc gcattgtggg
     2161 tgatggcaga aacgatgctg gagagcctgc gctgatgccg tttccctgag tgagggaatc
     2221 attcagcttt cgttcccgaa aaacacacat ttatttgtta tttaaggagt tatctcatga
     2281 aaaccgtttc tcagaatacc cccacaattt attcagctac aacaccagag aataatccgc
     2341 ctcagttggt tgccagcctc gtccctgatg aacagcgcat cagcttctgg ccgcagcatt
     2401 ttggcctcat tccacagtgg gtcaccctgg agccccgtgt cttcggctgg atggaccgtc
     2461 tgtgcgaaga ctactgcggt ggtatctgga atctgtacac cctgaacaac ggcggggcat
     2521 ttatggcccc cgaaccggat gatgatgatg acgaaacatg ggtactgttc aatgccatga
     2581 acggtaaccg cgctgaaatg agcccggaag ccgccggtat tgccgcctgt ctgatgacgt
     2641 acagccatca tgcctgtcgt acggagaatt atgccatgac ggtccattat taccggttgc
     2701 gggattacgc cctgcagcat ccggaaggca gcgccattat gcgcatcatt gactgaaagg
     2761 ggccggaata atgcaacaga tttcctttct gcccggagaa atgacgcccg gcgagcgcag
     2821 cctcattcaa cgggccctga aaaccctgga ccgccatctt catgaacccg gtgtggcctt
     2881 cacctccacc cgtgcagcac gggaatggct gattctgaac atggcgggac tggagcgtga
     2941 agagttccgg gtgctgtatc tgaacaacca gaatcagctg attgccggtg aaaccctctt
     3001 caccggcacc atcaaccgca cggaagtcca tccccgggaa gtgattaaac gcgccctgta
     3061 ccacaatgcc gctgccgtgg tactggcaca caatcacccg tccggtgaag tcacacccag
     3121 taaggcagac cggcttatca ccgaacgtct ggtacaggca ctgggcctgg tggatatccg
     3181 ggtgccggac catctgatag tcggtggcaa ccaggttttc tcctttgccg aacatggtct
     3241 gctttaaccc gtcacaacca catcacacct gttttcactt ttatcttctg tcttcagagg
     3301 tatcccatta tgaaaattat cacccgtggt gaagccatgc gtattcacca acaacatccg
     3361 acatcccgtc tttttccgtt ctgtaccggt aaataccgct ggcacggcag tgctgaagcg
     3421 tataccggtc gtgaagtgca ggatattccc ggtgtgctgg ccgtgtttgc tgaacgccgt
     3481 aaggacagtt ttggtccgta tgtccggctg atgagcgtca ccctgaactg agtgggaatt
     3541 ctgatgagca gaattatcgc cactaccgga ccattcttag ccgattttct gtaaggattt
     3601 tatcgtgtca gacacactcc ccgggacaac acttcccgac gacaatcacg accgcccctg
     3661 gtgggggctg ccctgcaccg tgacgccctg tttcggggca cgtctggtgc aggagggtaa
     3721 ccggttgcat taccttgccg accgcgccgg tatcagaggc ctgttcagcg atgcagatgc
     3781 gtaccacccg gaccaggcct ttccgctgct gatgaaacaa ctggaactca tgctcaccag
     3841 cggtgaactg aatccccgcc atcagcatac cgtcacgctg tatgcaaaag ggctgacctg
     3901 caaagccgat accctcagca gttgtggtta cgtttatctg gctgtttatc cgacgcccga
     3961 aatgaaaaat taactctcca gaatagcctt ctgctacggc ctggtgtttt caccacgcca
     4021 cttttccatt tttatatctg catatcagga aaatcttcag tatgaaaaca ttacctgtat
     4081 tacccgggca ggcggccagt tctcgcccgt ctcctgttga aatctggcag atactgctgt
     4141 cccgactgct ggaccagcac tatggcctca cactgaatga cacacctttt gccgatgaac
     4201 gtgtgattga gcagcatatt gaggcaggca tttcactgtg tgatgcggtg aactttctcg
     4261 tggaaaaata cgcgctggtg ctaccgacca gccgggattc agcgcctgta cccgctctca
     4321 gttaataaac agcatcgata tcctccgggc tcgcagggcg accggcctga tgacccgcga
     4381 caattacaga acggtaaata acattaccct gggtaagtat ccggaggcga aatgatgaca
     4441 ctggaagccg acagcgttaa cgtacaggcg ctggatatgg ggcacattgt cgttgacatt
     4501 gatggtgtta atatcactga actgattaat aaggccgctg aaaacggtta ttcactccgc
     4561 gtggtggatg accgtgactc taccgaaaca ccggcaactt atgccagccc tcaccagttg
     4621 ctgtaagaca atgcagtgat gcagggtata gtgccattaa ccacagaaac agcactgttg
     4681 tcctgtatta agcaggcaag agaagatgcc cttaccctgc gccatctgca gcttctgcac
     4741 cagaaccggt gaatctcact caacaggctt catttcccct gacgccagcc tgaatacagc
     4801 tggcgttttc atttataaac agaaaggaaa accgataata atggaatgga cgccccgacc
     4861 atgaagcaac ggtaggatat tactgataaa ccttccagtg gaggtgcgtt atggccgttt
     4921 taacgattag cattgacctg acaaaaaatg aattccaaat ccacggtctg ggtaggaaca
     4981 gaaaaatcta agctcagaaa acggattaaa gtaagcgtca acggagcacc gtattgacgc
     5041 ttatttattg gtgagaacta cgttccatgg caggagttcg tcaacacggt tggagggcca
     5101 ttccggcagt atgctcagaa tatggcgcag atacgcttcc ggttcaaccg ttcagacggc
     5161 aggtgcctgc ttttccggca acaagcagga atctcagcgc tgaagcagga attagaggta
     5221 aaaacgccct accgggccat gaatcacccg gtcattggag tagtaaccaa agcagattta
     5281 gccagcatgg aacaaatatc tttggtgaaa tgttggttac gggaagctgg agcgcacaac
     5341 gtgttagtaa ccagtgcggt taataacaat ggagttacag aactttttgc cttgctgcat
     5401 acagaagaag gctgccgcta atttcgatag gttctcagat gagaatgatg acggtgatac
     5461 acagataaga atcaatgagc aatgagggtt gccgggcaac cctcattgaa taaaacagga
     5521 attacttcgc ttcgccgttt ttcatttcgc ccatagcttt cagctttttg gagatatcgc
     5581 ggcgttcttt ggagagctcg gcatttttga tgatgtaatc gtcaacgcga tcttcatagt
     5641 cacctttcat gctggcgatg atgccctgga ttgcttcaac gctcatccct ggcttgatgt
     5701 aatcgctcag gttgtccagc aacaggacgc gcttctggtt gtcacggatc tttttctcaa
     5761 cgtcctgaat ttcacgttgc agtttgttct tacgacggaa cagacgaaca aattccagta
     5821 cgtcctggaa tgaaggcttg gtagtttcca tttttatacc cctgataatg tgagagtcgg
     5881 attcgtttaa gcaaccgcta ttcgttaggg ccaacattac tcatagctgc cgcagatgac
     5941 aatgctttta tcccttatca ctatcatacc ctttatcctt gctgaatcga agcagcagca
     6001 agatgattct gaagttcagg aattatttgc gcaaggcccg gcaaatcaga ctcgatagca
     6061 actcaagctg atgtgccgtt tccatgttca gccacacata accatagatt ggtgtttcca
     6121 caaccttcag gtcatggtgg ttattcagca actgacgcag ctcttccaca gcatcgttca
     6181 atttttccgt attggcaaaa accggctgcg gattaccttc gtacagcgca tgaacaaggc
     6241 tcagcagtat ttgctgcatc atgtgctggg tatcacgaag tttttgcgcg ttcaataaca
     6301 cgaaatggct ggggcgcgtg gcccagtatg cattgatttg caactccagc atacaaacca
     6361 gattgcggtt aatggtctgg ataccttcat atatcgattt tggaatacga gtttctttgc
     6421 tggcgggcgc aatcagccca cgcattttca cggcatcggt caggagtttt tgtagatggc
     6481 tttccagacg tgggcgttcg agtaagttcg gtgagaatgc agattgatag acccgattat
     6541 actcggtcag acttttcgcc agttgaatgc gccagtggat gaacgcccgt tgtggccaga
     6601 taccggtaaa caacattgcc agtaaagagc cgaggatcac atcgccgctt cgccataacg
     6661 ccgtatcaat ttcacctgtc ggggaaccca caacaattgc cagcgtcacc ccaatcaata
     6721 atccttgata cggtttcttg cccagcgcca gccaaccgca aagaaacatc gccgccgcgc
     6781 accagactaa catcagcggt aacgaaatta actccagttg cagagcgata agacctaaaa
     6841 tcgaccccaa caccgtaccg ccaatacgtt cgaaggcacg agggacaacg ttcccccaga
     6901 acgagattgg ccccataatc accaccatgg tgaccagcgg ccaggtgcct tccgggatag
     6961 taaacaggcg gataatgaga aaagtgagca ggaatgccag cgcgacccga gtaccatgca
     7021 caatgcggta atggcggtat acccggattt caaacgggct taatgactta ttggcacgca
     7081 cgcagaaact ccgttaatga agattaaaag cgctcataac acatctgtcg gtcagaaggc
     7141 gcgcctctga attatccgac ggacaaaccg ggaacagtgt aatttcccgg caggagcgaa
     7201 aaaattgaaa ggcgcatctt actcttttcg ctttcaaaaa aagagtggtc ttcgcgtcaa
     7261 cacaccgccc tgagatgaat tagtgatgtt ttggctgcac cgcaacatac atttcgatat
     7321 cccagtaccc atcttccgcg ccattgttca agtaaacctc gaagcagggc tttggtaaca
     7381 tttcataagc actgtcctgc aagaggctat taaagaactg ataccagggt ttagcaaaat
     7441 catcaccgac tacacgagcc accgccaccg catattgacc acctgaaatt tctgtcagaa
     7501 tgacgccctc actgttttcg gggagcgtaa agttattcgg taccgtcacg acggtgtcgc
     7561 agcgtaattt ttcggcgggt gtttcatccg gattgtcgta atagacagca acccactcct
     7621 tcggcacaat atttttgcta tctacccaca tcatcaactg ctcaaaacct ttctttaccg
     7681 tctgttccca cgggccaacg agatggaaac ctgcaacggt acgtttatct tcctgcttaa
     7741 tctcgtagtt catgacgcct ccattattac tgtttttata tacagtatag ttgcaatatt
     7801 aaaaccacaa ggaatgagtg ttgattatgc gagcagactc gcactcctgc cagtctgctg
     7861 caaaagaaag gtcaggcctt atggtggaaa taatcactca ggcgagaaaa catgctgcct
     7921 tccccgacag attccagggt aaccagcggc cagtgcgcca cctgtttatc acggtcgtaa
     7981 agttcaattt cccctacccg ctgatgggcg ctaattggcg cggtgagctc tttaccatca
     8041 agggtatatt tggctttgat atgtggaatt tcggctttcg gtagcaccat ccagaactct
     8101 tgttccgttc ccagggcgat attttcttta tcgccatacc agatgcgttc cgtaccgacc
     8161 tttttcccac ggtgcaaaat ttgcaccgta gtaaagtttt gttgccccca acgcagtaat
     8221 tttcttgcct cttcctcacg accttttgca ctgtcagccc ccattaccac tgcaatgaga
     8281 cgacgctgcc catctacagc cgaagcaatg agattaaacc cggcaccaga agtatgaccc
     8341 gttttcaggc cgtcaacatt catggtttta tcccacaata acccgttacg gttttgctgg
     8401 gtgataccgt tccaggtgag acttttctca ctgtacatat gataaaactc gggctcgccg
     8461 tggatgatag cgcgagaaag cacagctaaa tcataagccg agctatgctg acctggtgca
     8521 tccagaccat gcactgtttc aaaatgcgta tccttgagat gcagcttctc ggcatagttg
     8581 ttcatcattt caacaaactg ccgttgccca ccggcaatat agtcagccag tgcaacacaa
     8641 gcgtcatttc cggaatccac aattaaacca cggcttaaat cacgtaccga tacgcgatcg
     8701 ccctctttca aaaacatcag tgaagaaccg acaaacactg gattatcttt cgcccacgca
     8761 tcgcgcccca cggtgacaat atcgtctggc gtaatgcgat gactatcgat agcgcgatcc
     8821 acgacatagc ccgtcatcag ctttgtcagg ctggcgggat tgcgctgttg atgctcatta
     8881 cccgcggtga ggatctgacc ggtggtgtaa tccatcaata cccaggagcc ggcatgaatc
     8941 tctggaggct gaggtgaaaa aggaatgttt tccgccgcaa aaccagacga taagttaaaa
     9001 atgaacaaag aagcagcaat aataagacgg cgtttcaaca gcaaaccctc aggagtttca
     9061 aatagctgtt ctttttacgg aaatacttat gaactggctg gaataaagtg caagaaaatg
     9121 tgactaccct ctcattttta tctgacatga tctgttgcca ctcgctgtca aattgtcgcg
     9181 ctaaagctga ttagcacggt gatatttgat actctggcag acagcagaaa caacggattt
     9241 aacctaatga tgaatgacgg taagcaacaa tctacctttt tgttttcacg attacgcgca
     9301 acacccctga gagccgccat gaacaagggt ttcataattt gtgattttag tttggtacgc
     9361 aatttggtac acaacacaat tttcacttca gtgggtagtc atcaaactca ccataacgag
     9421 catcgttgat gatgtatgtt ataaccacaa acatcacatc tggactagtt ccgttttcat
     9481 gagcgggtat ttgtttacgc ctccctttct gcaaatccta aagatgagat tttggggttt
     9541 tgggatatct tttatcctca gttctctatc cattatgcaa accagtaatg aaccatcaca
     9601 gtgtgatagt gaggcgtctg ccacgataag cgcagagaaa tgatgcaatg gtgggcggac
     9661 tggcttgatg aaaaggtaga gtgagcgacc ttaaactatc gaatagaaca aagtcttgca
     9721 atccagtgca aagctttgtg cgcctcagtt ttgtctaagc gttctactga aaacatagta
     9781 aaatcggtaa cggctggaaa tcattcaata ctcgcactat cgaaagttcg ccagccagcc
     9841 gtggcacgtt cttgcatacg acgtgccgcg gatcctatta ttctagtatg aaatccctaa
     9901 ccgatttcaa attctccaga ctgctcagat tctcttttat cccacgctgg cgctgataaa
     9961 tctcgtcatt gcaatcgacc tgcgctatcg ctgccgcagt tcttccagtt tctgcatcga
    10021 taactccatc ttctgagcaa taaacctcac ctatgcaata ttttatgtca attatttcaa
    10081 ttgcattaat atcttgaatg gattacatag agttaatgta tccagtactc cctattctct
    10141 tgcatataat acatgttgca acttacatct cagcgctatg aaaaaacacc acctctctct
    10201 ttatgaaatt ctggatttac caagcgctaa tttatcattt caatctacct ttaagcattg
    10261 catttatctc cctacgaggt catactttag gaagttaaat atgaatgata atatacctac
    10321 agcgcgaaat cacaaacaat cgacttgtat tacagaaaaa acatgcctat ctttttaaac
    10381 ttcacagcag gcagtatctt acctgagaat gagctagcat ctttacgtta tattgtgcag
    10441 caaaatcaaa atgatactgt aatcataaaa gaacgttata aaatggatat ccgttatatc
    10501 gaatcagtca atggttttac agtaaatcct gtatgcagta atcatttctc catatttatg
    10561 gcgagacaaa acactattgc tcgcaacctg gaacagcaga tcaacaacgg acgaagtttt
    10621 gcacaaatat ctcaggattt tatgcttcaa ttatcttcaa atataggatg gaaaaaaggg
    10681 gccgaaaacg cccttaaaaa taaaatccat tctcattcat ttgttgtaaa tcctgatgaa
    10741 ttctcttgcg acacacaatt tcttaagtgc ccaataacat tatgcgttcc agaaaaaggg
    10801 gtttttgtca agaacgcact gaactccaac atatgcactc tttatgataa gtctgcgttc
    10861 atgaatctca caagagaaca tctaccccac cctctcagca gggaaaagat agtaaaagaa
    10921 atgattattg aaaggaatat gtgttatttt gacaccataa gtcagcattt cataattatg
    10981 gatacagacc aacagaaaca gcattgtaaa taaaatgtaa taattacata ctattagtga
    11041 ttctcatgca tcgtaagcgg ctcgccagaa ccgtattgat atttactgag cgctcagatc
    11101 aactttccat ggcaacagat cgcgtacccg gtttgccggc cagtcctgga tatgctcccg
    11161 ccgacaatat taatggccag gaaatgttta aaacaatgat tctgtttcct aaccatcaag
    11221 tgtatatgct ggctcaatta ctcaatatct atgggttgag ttctacccaa agtggctctg
    11281 tagtcgcttc ttcttacagt atgttcaggt gttgcaggca cagttgtgga gcgtagatgc
    11341 gttgttggtt taccatgctc tggtacataa aaaacgccag tgccatggta acctgatata
    11401 gaaacgttaa ttccgcgacg ttcaaactcg gcatagacgt gttctgctaa agacctcttt
    11461 tgaccaaata atgccctggc caaccagccg ttattcatat ttgcggattt ttctatttca
    11521 tcaggagaga agtctttgtt ttttattatg ttggctgagt tacaggatgt taatctgata
    11581 tcatctattt catgaagatt atgctgaaca atgcggtcga caatatctga cggtgataaa
    11641 aattcatctc cgcacttaag aagaggtaga ccagcagaac catgcccaga cagataaatt
    11701 ttgttgtatt ttcctagtgt taaatcatct ggcaggatgg ttttcatctc tgtcgctgta
    11761 atacttatag ctgcagcagc aacaacatcg ctattacttg attgtagatg gcttttattt
    11821 gctccaggat atgtaaactc cattttcctt ttatcaaaat cttgttttgt agcatcattc
    11881 aggaataata aatcatacgg ttttttcccc gttgtgactc ttgaaacatt ttggccaaga
    11941 atattaagag tatagtcatt aacggatttc attcctaaaa aaacaagtaa tccttctttc
    12001 tcttgcgctt tttcgattgt tctccctgct aaagttattg ggatggattg agattgtttt
    12061 gtcggaactg atacgctggc gctaataggt agtagggttc ttatgctaaa catacaacct
    12121 ctttcttaat tttcgaacct gtttaggatt ctgtgtaaat tcaaaataaa cctgtctgaa
    12181 cattcctaac aaacatccac cggacatgac aacaaaaacc ggagccggac tccggttttt
    12241 gtgaagctgt cggctatttc attccgccaa tattttccca cctcccgtca gcacgcagaa
    12301 tttgcagcgg tcttaccacg cactgtatct gctttttatc cgcatccagt atcaccacct
    12361 gcgtgattac cctgtcctgc tccgggataa t
//
LOCUS       DAZKIP010000121        12015 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZKIP010000121 DAZKIP010000000
VERSION     DAZKIP010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12015
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.122"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            15..617
                     /gene="yebB"
                     /locus_tag="ACHOUK_003784"
     CDS             15..617
                     /gene="yebB"
                     /locus_tag="ACHOUK_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFP9899141.1"
                     /translation="MNINYPAEYEIGDKVFTCIGDALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(619..1140)
                     /gene="ruvC"
                     /locus_tag="ACHOUK_003785"
     CDS             complement(619..1140)
                     /gene="ruvC"
                     /locus_tag="ACHOUK_003785"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFP9899142.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(1175..1915)
                     /gene="yebC"
                     /locus_tag="ACHOUK_003786"
     CDS             complement(1175..1915)
                     /gene="yebC"
                     /locus_tag="ACHOUK_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9899143.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(1944..2396)
                     /gene="nudB"
                     /locus_tag="ACHOUK_003787"
     CDS             complement(1944..2396)
                     /gene="nudB"
                     /locus_tag="ACHOUK_003787"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFP9899144.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(2514..4286)
                     /gene="aspS"
                     /locus_tag="ACHOUK_003788"
     CDS             complement(2514..4286)
                     /gene="aspS"
                     /locus_tag="ACHOUK_003788"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFP9899145.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLRSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPAALAELSIQVVKKAENN"
     gene            4596..5162
                     /gene="yecD"
                     /locus_tag="ACHOUK_003789"
     CDS             4596..5162
                     /gene="yecD"
                     /locus_tag="ACHOUK_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFP9899146.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILNAL"
     gene            5159..5977
                     /gene="yecE"
                     /locus_tag="ACHOUK_003790"
     CDS             5159..5977
                     /gene="yecE"
                     /locus_tag="ACHOUK_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFP9899147.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNQVIL
                     DSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            6030..6425
                     /gene="yecN"
                     /locus_tag="ACHOUK_003791"
     CDS             6030..6425
                     /gene="yecN"
                     /locus_tag="ACHOUK_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFP9899148.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            6466..7209
                     /gene="cmoA"
                     /locus_tag="ACHOUK_003792"
     CDS             6466..7209
                     /gene="cmoA"
                     /locus_tag="ACHOUK_003792"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFP9899149.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            7206..8177
                     /gene="cmoB"
                     /locus_tag="ACHOUK_003793"
     CDS             7206..8177
                     /gene="cmoB"
                     /locus_tag="ACHOUK_003793"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFP9899150.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(8342..10771)
                     /gene="torZ"
                     /locus_tag="ACHOUK_003794"
     CDS             complement(8342..10771)
                     /gene="torZ"
                     /locus_tag="ACHOUK_003794"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFP9899151.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGYNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFAELAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAQLRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAVQA"
     gene            complement(10796..11896)
                     /gene="torY"
                     /locus_tag="ACHOUK_003795"
     CDS             complement(10796..11896)
                     /gene="torY"
                     /locus_tag="ACHOUK_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFP9899152.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGFLMAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFEAHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKLLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         2749 a         3186 c         2933 g         3147 t
ORIGIN      
        1 aatggaatga caacgtgaat attaattacc ctgctgaata tgaaattgga gataaagtct
       61 ttacatgtat aggtgatgcc ttatttggtc aaatatcagc tgcatcaaat tgctggagta
      121 atcacgtcgg gatcattatc ggtcataacg gtgaagactt cctggttgca gaaagccggg
      181 ttcccctttc aaccatcacc acgctatctc gttttattaa acgttcgtct aatcaacgct
      241 atgctataaa gcgattagac gctgggctga cagaacaaca aaaacaacga attgttgaac
      301 aggttccctc ccggctacgc aaactttacc acaccggttt taaatacgaa tcttcgcgcc
      361 agttctgttc aaaatttgtt tttgatattt ataaagaagc gctatgtatt ccggtgggtg
      421 aaatagagac gtttggagaa ttgttaaata gcaatccgaa tgcaaaactc actttctgga
      481 aattctggtt cctgggttct attccgtggg agcgtaaaac cgtcacgcca gccagtttgt
      541 ggcatcatcc aggtttggtg ttgattcatg cggagggagt ggaaacgcct cagccggaac
      601 tgaccgaggc ggtataactt aacgcagtcg ccctctcgcc aggttcagcc gcgattcgct
      661 catctgcatc gcattctgac taacgtggca gtgggtgatg gcaatcgcca gcgcatcggc
      721 ggcatccgcc tgtggattag cgggcagttt cagcaaggtg cggaccatat gctgcacctg
      781 gcttttttcg gcactaccaa tacctaccac cgtttgcttt acctgacgtg ccgcgtattc
      841 aaatactggc aactcctgat tcaccgccgc cacaatcgcc acgccgcgcg cctggcccag
      901 tttcagggct gagtcagcgt tctttgccat aaagacttgt tcaatggcga aataatcggg
      961 ctggaactgg gtgatgattt ccgtcacgcc cgcatagatg agtttcagac gagacggtaa
     1021 atcatccact ttggtgcgta tgcatccgct acccaggtag gacagttgcc tacctacctg
     1081 gcggatgacg ccgtagccgg tcacgcgcga acccggatca atgccgagaa taatagccat
     1141 cacgcgtctc cgttttgctg tttagcaggc ctcatcagag agtcgctgcg acctcatcag
     1201 agatttcacc gttatggtaa acttcctgca cgtcgtcgca atcttccagc atatcgatca
     1261 gacgcatcag tttcggtgcg gtttctgcat ccatatcagc tttggtagac gggatcatgg
     1321 aaacttccgc gctgtctgct ttcagacctg ccgcttccag agcgtcgcgc actttaccca
     1381 tttcttccca tgcagtgtag acatcaatcg cgccgtcatc ataggtcaca acgtcttcag
     1441 cacctgcttc cagtgctgct tccatgatgg tgtcttcatc gcctttctcg aaggagatga
     1501 cgcctttttt gctgaacaga taggctacgg aaccatcagt accgaggtta ccgccacatt
     1561 tgctgaatgc atgacgcact tcagcaacgg tacggttgcg gttgtcagac agacattcaa
     1621 tcatgattgc cgtgccgcca ggaccgtaac cttcgtagat gatggtttcc atgttcgcat
     1681 catcatcacc gcccacacca cgtgcaattg cgcggttcag agtgtcacgg gtcatgttgt
     1741 tagacagtgc tttatcaatt gctgcacgca gacgcgggtt agcgtccgga tcgccaccgc
     1801 ccagcttagc cgcggttacc agctcacgaa tgattttagt gaagatttta ccgcgcttag
     1861 catcctgcgc agctttacga tgtctggtgt tggcccattt actatgacct gccataaaaa
     1921 tatctccaga tagccctgcc tgttcaggca gcgttaatta caaactgttc aatcgcctgc
     1981 cggttgctcc aggacttagt gagcgccgcc gcagcaggcg catcaagcca cttgtaagcc
     2041 agatgttcag tgaaaacgat ctgccgctca tgcggaagcg caagacagaa ccatgattcc
     2101 gtattacgcg tcacgcccgg cgcatagcga tgacgtaaat gtgaaaaaat ttcaaactct
     2161 accgtgcgct gacagtcaat taaggtcagt tgttcagcga caacatcaat ggtgacctct
     2221 tcctttactt cgcgcatggc agcttgcggc gcggtttcac cctcttccac gctgccggtt
     2281 accgactgcc agaaatcggg gtcatcacgc cgctgcaaca tcagcacccg tttcgtatct
     2341 tgtgcgtaga tgaccactaa gatcgaaacg ggacgcttat acactttatc cttcacgctg
     2401 cctcttcgtt gactgccttc gctcatccca ttcacatagt tatctatgct catgggagtt
     2461 cactcagttg ccgcctcgat gcaacgcgaa tgatttcgtg tatttgagtc atatcagtta
     2521 ttctcagcct tcttcacaac ctgaatgctc agctcagcca gtgcagccgg gttagcaaag
     2581 ctcggtgctt cagtcatcag acacgctgcc gccgtggttt tcgggaaggc gataacgtca
     2641 cggatattgt cggtgccggt cagcagcatg gtcagacggt caagaccgaa tgccagacct
     2701 gcgtgcggcg gagtaccgta tttcagagcg tcgagcagga agccgaattt ctcgcgctgt
     2761 tcctcttcgt tgatacccag aataccaaac accgtctgct gcatatcacc attatggata
     2821 cgtactgaac caccgcccac ttcgtaacca ttgatgacca tatcgtaagc gttcgccacc
     2881 gcattttccg gtgcagcttt cagctctgca gccgtcatgt ctttcggtga ggtgaacgga
     2941 tggtgcattg ccgtcaggcc gccttcaccg tcgtcttcaa acatcgggaa gtcgataacc
     3001 cacagcggtg cccatttgct ttcgtcggtc agaccaaggt ctttacccac tttcaggcgc
     3061 agtgcgccca tcgcgtcggc aacaattttc ttgttgtcgg caccgaagaa aatcatatcg
     3121 ccatcttgcg cggcagtacg atccaggatg gcttcgatga tttctgcatt aaggaacttc
     3181 gctaccgggc tgttgatacc ttccagacct ttcgcgcgtt cgttaacttt gatgtaagcc
     3241 agacctttcg cgccgtagat tttaacgaag ttaccgtatt cgtcgatctg cttacgggtc
     3301 agcgatgcgc cacccggaac acgcagagcg gcaacgcgac ctttcggatc gttcgccgga
     3361 cctgcaaata ctgcaaactc aacagatctc agcagatcag caacgtcggt cagttccatc
     3421 gggttacgca gatccggttt atcagaacca taacggcgtt cggcttccgc aaaggtcatt
     3481 actgggaaat cgcccagatc cacgcccttc acttccagcc acagatggcg caccagcgct
     3541 tccatcactt cacgcacttg cggcgcggtc atgaaagaag tttccacatc gatctgagta
     3601 aattcaggct gacgatcagc gcgcaggtct tcgtcacgga agcatttaac gatctgatag
     3661 tagcggtcaa agccggacat catcagcagc tgtttgaaca actgtgggga ttgcggcagt
     3721 gcgtagaatt tacctttgtg cacacgagaa ggcaccaggt agtcacgcgc gccttccggc
     3781 gtggctttgg tcagcatcgg agtttcgatg tcgaggaaac cgtggtcatc cataaaacgg
     3841 cgcaccaggc tggtgatttt agcgcgggtt ttcaggcgct gagccatttc cggacgacgc
     3901 aggtcgaggt agcggtattt cagacgcgct tcttcggtgt tgacgtggtt agagtcaagc
     3961 ggcagaacat ctgcgcggtt gatgatagtc agcgaggacg ccagcacttc gatttcgccg
     4021 gtcgccatat cgcggttaat atttttttcg tcacgcgcac gtacggtgcc cgtgacctga
     4081 atgcagaact cattacgcag ttcagaggcc agctttaacg cgtccgcacg atccggatcg
     4141 aaaaatacct gcacgatacc ttcgcggtcg cgcatatcga tgaagatcag gctaccaaga
     4201 tcacgacgac ggttgaccca accacacaga gtcacctgct gccccacgtg ggacaaacgg
     4261 agctgtccac aatattctgt acgcatgaga tatcccttaa cttagctgcc ggcggatgcc
     4321 ccctgctgcg caggtgacca agtcgcagcg ttagctgtat gtcacaactg aatgaaaaaa
     4381 ggcggctatt atactggaaa ttctgccgca ccgtaagagc ctggcccgcg ctggaacgcc
     4441 tcgttaccac tttatatcgg gcctgaaatc agactctacg ccagtttgct ataaaggtgt
     4501 tgcccgaact catacaaatt aacaaaattt gtcgttccgc catcggctaa tcgcattaag
     4561 gtgtgaggca cgattttgtt ttttcaggag tcatcatgct tgaacttaat gctaaaacca
     4621 ctgcactggt ggtgatcgat ttacaagaag gcatcttacc ttttgccggt ggtccacata
     4681 ctgccgatga ggtagttaat cgcgccggga agctggcggc gaaatttcgc gccagcggtc
     4741 agcccgtgtt tctggtgcgc gttggctggt ctgccgatta cgccgaagca ttaaaacagc
     4801 cggttgatgc cccttccccc gctaaagtgt tgcccgaaaa ctggtggcaa catccagctg
     4861 cattaggtgc aactgacagt gatatcgaaa taatcaaacg tcagtggggt gcgttttacg
     4921 gtacggatct ggagttgcaa ttacgccgcc gaggtatcga tacaatagtg ttatgtggga
     4981 tctcgaccaa tatcggagtt gaatccaccg cccgcaatgc ctgggaactt ggctttaatc
     5041 tggtgattgc cgaagatgcc tgtagtgccg ctagcgccga gcagcacaat aacagtatca
     5101 accatatcta cccgcgcatc gcccgtgtgc gtagcgtgga agagatcctc aacgcgttat
     5161 gatttacatc ggtctaccgc aatggtcgca tcctaaatgg gtgcggttgg ggatcaccag
     5221 ccttgaagag tatgcccgtc actttaactg cgtggagggc aacaccacgc tttacgccct
     5281 gccgaaaccc gaggttgtcc tgcgctggcg tgaacagacc acagatgact tccgcttctg
     5341 ttttaagttt ccggcgacca tttcgcatca ggcagcatta cgacattgcg atgatttagt
     5401 gactgaattt ttgacccgca tgtcaccgtt ggctccgcgc attgggcaat actggctgca
     5461 actgcctgcc acattcggcc cacgggagct gcctgcgctt tggcattttc tcgattctct
     5521 ccctggcgaa tttaattatg gtgtggaagt ccgccatcca cagtttttcg ccaaagggga
     5581 agaggaacaa acgcttaatc gcggtttaca tcagcgcggc gttaatcagg tgattttaga
     5641 cagccgcccg gttcatgcag cacgtccaca cagtgaagct attcgcgacg ctcaacgaaa
     5701 aaaacctaaa gttccggtac atgctgtact gacggcgaca aatccgctga tccgttttat
     5761 cggtagtgat gatatgacgc aaaaccggga attatttcag gtctggttac aaaaattagc
     5821 gcagtggcat cagaccacta cgccttatct ttttttacat acgccagata ttgcccaggc
     5881 cccggaactg gtacataccc tgtgggaaga cttacgtaaa acgcttccag agatcggagc
     5941 agttccggct attccacagc aatcttctct tttctgaatt tgccacctat catagacagg
     6001 tgccatcggc cattttaaag ggagtttgta tggtaagcgc gctgtatgcc gttttaagtg
     6061 cgttgttatt aatgaagttc tcttttgatg tcgttcgcct gcgaatgcag taccgcgttg
     6121 cctatggtga cggtggtttt agcgaactgc aaagcgctat tcgcattcat ggtaacgcgg
     6181 tggaatatat tcccatcgcg attgtgctga tgctgtttat ggaaatgaat ggcgcagaaa
     6241 cctggatggt gcatatttgc ggcatcgttt tgcttgctgg tcgtctgatg cattattacg
     6301 gttttcatca ccgtctgttc cgctggcgac gttccggcat gagcgccacc tggtgtgcgc
     6361 tgttgctgat ggtgctggcg aatctttggt atatgccctg ggagttggtt ttctccctgc
     6421 gttagcgcac aatacgccac tttctttttc ccggattttt acgttatgtc tcaccgcgac
     6481 acgctatttt ctgcccctat cgccagactg ggcgactgga cctttgatga acgggtagct
     6541 gaagtcttcc cggatatgat ccagcgttcc gttcccggct attccaatat tatttccatg
     6601 attggtatgt tagccgagcg cttcgttcaa cctggtacgc aggtttacga tctgggttgt
     6661 tctctgggcg cggcgacgct ctcggtgcgt cgcaacattc atcatgataa ttgcaaaatt
     6721 attgccatcg acaactcccc ggcgatgatt gaacgctgcc gtcgtcatat tgacgcctat
     6781 aaagccccta cgccagtaga cgtcattgaa ggtgatattc gcgatatcgc cattgaaaac
     6841 gcatcgatgg tggtgctgaa ttttaccctg caattcctgg aaccttccga gcgccaggcg
     6901 ttactggata aaatttatca agggctgaac cccggcggcg cgctggtgct ttcggaaaaa
     6961 ttcagtttcg aagatgccaa agttggtgaa ctgttgttca acatgcacca cgactttaaa
     7021 cgtgccaacg gttacagcga actggagatc agccagaagc gcagcatgct ggaaaacgtg
     7081 atgctgaccg attccgtgga aacccataaa gcacgcctgc ataaagccgg ttttgagcat
     7141 agcgagctgt ggttccagtg ctttaacttt ggttcactgg tggcattaaa agcagaggac
     7201 gctgcatgat cgactttggt aacttttatt ctctgattgc caaaaatcat ctttcacact
     7261 ggctcgaaac gctgcccgcg cagattgcta actggcagcg cgagcagcag cacgggttgt
     7321 ttaagcagtg gtcaaatgcg gtggagtttc tacctgaaat taaaccgtat cgtcttgatt
     7381 tattgcatag cgttacggca gaaagcgaag agccactgag cgcagggcaa attaaacgca
     7441 ttgaaacgct gatgcgcaac ctgatgccgt ggcgcaaagg gccgttctca ctgtatggcg
     7501 tcaacatcga taccgaatgg cgttccgact ggaaatggga tcgtgttctg ccccatcttt
     7561 ctgatttaac cggacgcacc attctggatg tcggctgcgg cagcggttat cacatgtggc
     7621 gcatgattgg cgcaggcgcg cacctcgcgg tggggatcga ccctacgcaa ctgtttctgt
     7681 gccagtttga agcggtgcgt aaactactgg gtaatgatca gcgcgcgcat ttgttaccgt
     7741 taggtattga acaacttccg gcactgaaag cctttgatac cgtcttttcg atgggcgtgc
     7801 tttatcatcg tcgttcaccg ctggagcatc tctggcagtt gaaagatcaa ctggtcaatg
     7861 aaggtgaact ggtgcttgaa acgctggtta ttgatggcga tgagaacacg gtgctggtgc
     7921 cgggcgatcg ttacgcgcaa atgcgtaatg tctatttcat cccttccgcg ctggcgctga
     7981 aaaactggct gaagaagtgt ggttttgtag atatccgcat tgcggatgtg agcgttacca
     8041 ctacagaaga gcagcgacgc actgaatgga tggttaccga gtctctgtcc gatttcctcg
     8101 acccacatga tccgagtaag acagttgaag gttatcctgc gcctaaacgc gcggtgctga
     8161 ttgcgcgcaa gccgtaaagg tctggtatta ctgccggatg cggcgtgaac gccttatcca
     8221 gcctacaaaa ccttgctaat tcaatatatt gcagggacta tgtaggcctg ataagcatag
     8281 cgcatcaggc agctttatgt ttgcataacc tcagcgcccg tttccgggcg cttttcacgt
     8341 cttatgcctg aacagctggc tgatcaaacg ccgttaactt cggtgcgtta cccgtatatt
     8401 tttcgatata caccagcgca gagttaccgg cacaggcatt tgccagctgc gagctgggga
     8461 tatccgccgt taacacgttc gcactgccgt ttttacacaa gccattttcc agatctggcc
     8521 atgcaccttc atgcacgcat accacgcctt ttttgatccc gtcagtcacc acagcgcctg
     8581 tcaaaatctg accacgtttg ttccagacac ggaccagatc gccattcgca ataccaaagc
     8641 gagcagcatc ttcggtgtga atagtgattg gttcacgatc ggcaaccgca tattttttac
     8701 gcagttgcgc atagttaagc tgactgtgta aacggtgtgc cggatgtgcg gtcagaagct
     8761 gtaactgctt ctcgtcggcg gtacctttcc actcatcagg cgcaagccag gttgggtgtg
     8821 ccgggcaatc cttatagcca aatttttcca gcgttttgga gtaaatctca attttgccgc
     8881 ttggcgtacc cagcgcattt ttcaccggat cggcgcggaa atcaccataa cgaacgtact
     8941 gttcgttctt ctcgctgcgg cgcatttcga tcagtttatt ttgctgccag aaggcattaa
     9001 acattggcat agtgacgcgt tgcgcacggg cacctttctg agcggcatca tagaaaaatt
     9061 tcagccacgc catttcatct ttaccttcgg tatagatctc ttttccgcct ggtttcagta
     9121 attccgcaag ttcggcaaac acgtcaaaat cgtttcgcgc ttcaaattgc ggagcgacag
     9181 cctgcttcat cggtacaata tgctggttgc tgtaatcacc ggtcatcgtc aagtcattgc
     9241 gctcaaacga tgtggtgatc ggtaatacga tatctgcatg tttagcagct gcggtccagt
     9301 agcattcaga aacgacgatc atctccggtt tctgccatgc tttgatcaga cgattagtat
     9361 cctggtgatg ggtaaagtta ccgccacccg cccaccagat cattttgata ttcggatacg
     9421 tttgttcttt accgttatgc tgatatttgc cgccaggatt ttccagcgca tcgacgatac
     9481 gggcaacagg aatcgccgtc attccgccat catcagcggc ctcgctggcc tgtccggcaa
     9541 tcgccgcaga catttccggc agcacgccgc caacacgcgt cgggttgcca ccgttggagt
     9601 aatgataaga gaaaccaaaa ccgccgcccg gtgtaccaat ctgccctaac atcgccgcca
     9661 gcgtcaccag catccagtgt ttctgttcac catattgctg gcgctgaatt ccccagcctg
     9721 ccatcagcat tgtgcgattc gccgccatca gttcagcgag tttgactatc tgcgcttccg
     9781 gtacgccagt aatttccgcc gtccaggccg cgctcttcgg agtattatcg ctcttacctg
     9841 tcagatactc ttcaaactgc ggatacccgg tggtgtattt ttcaaggaac actttatcgt
     9901 gtttgccttg tgtcatcagg gtatgcgcaa tccctaacat cagtgccacg tcggtgccca
     9961 tattcggcgc gatccaggtg gcattatagc caaagaactc gatggtttca gagcggatag
    10021 gatcaatggc aatcactggt ttgccagatt ttttcagctg atggaagtat tccagccctt
    10081 gctcatcggt actgctccag gcaattttta aggtattcag cgggttcatt ccccacagca
    10141 ccacaacctg gctgttttcc agaatcagcg gccaggaggt ctgctgttca tacacctcta
    10201 cagaaccgac cacatgcggc atgatcacct gtgctgcccc ggttgaataa tcgccgctat
    10261 gcccggaata accgcccgcc aggttcatat aacgttgcag taaggtttgc gctttatgca
    10321 acacgccaga agagcgccag ccgtaagatc cggcaaaaat ggccgatggt ccgttagctt
    10381 tacgaatacg ctcatgttgc tcatgaatca gttttaatgc ctgttcccag ctcacctgta
    10441 cataggtatc ttcgccacga cctttcgccg gttgcagtgg attgtcgaga tagctttttc
    10501 tcaccatcgg atgctgaata cgcgccgtgg tgtgtacctg atccgccgcc gtagactgta
    10561 aggagttcgg tatggttttc gccagcgcgc ctgtcgaaga aacaatcttg ccgtccttca
    10621 cttctacgtt catcgctccc caacgtcccg cggtgaggat tttaccgccc ttctcttctg
    10681 cccatgcggg tagcggtgct gccgatgtca ccaccagcgc tccagcggca ataccgctgt
    10741 gtttaataaa ttcacgtctt gttaatgtca taacttcctc cctgatcaac gaggatcact
    10801 gtttctcggt aatatctttg gcgttgtact ggaaataccg cgttaaaatg tccagttcgt
    10861 tttcgctcat gctggttcgt gcccccattc ctttggcaat ggacggccac gcattgacgg
    10921 tgtaatggtc ggcggcaata ggggcatgac aaccagcgca ataggtatcg tcaagttttt
    10981 cagcgtattg ccatagcagt ttacggtccg ctaatgcggg atcggtaagc gcaccctgta
    11041 aagacgcctg acgccattga ttgccgtatt cgtcagcctg ccattccccg tttaccgtga
    11101 gcgccttgat accttcttca cttaatgtgg ctagcgccag ccgttgacct gccgccaggt
    11161 agagcgtgtt ttcactgccc tgcatttgat aaccctgcaa cagaacgatc ggctgtttgc
    11221 cactggcatc aacgacggtg agatcggtac caggattcac ggtagccagc tcgcctatgc
    11281 gagaagtttt gaaaggataa atatgtgcgc cattagtcac tgaagtagcg gcctgacttt
    11341 ccagctcatg cgccgcgttg tcatccattt ttatttctgg cggaaaatgg gcaatgcctt
    11401 tatgacaatc gatacaggtt tcgccgccct tttgtgcttt gttatgcatt ttctgcgcag
    11461 attcactttg cgaggcaata tccatggcat caaaagaatg gcaactacgg cacgttgcag
    11521 agtcagttgc ttttaattct ttccatactg tttcggccat ttcctggcga tgagcttcga
    11581 acttatcgtc actgtctatt ttgccgctaa caaattcatg ataaatatct ttagatgctt
    11641 ttaatttagc gaataaataa tccatccctg actttggaat atggcaatcg gcacattccg
    11701 cacgtatccc tttctggttc gaaaagtgga cagttccctg atattcctca aaaggtttac
    11761 tcatcgagtg gcaggaaagg caaaatgctg tatccgacgt tttatgtaag gctttttgcg
    11821 ccatcaagaa cccgccacca ccaaccacaa ccgctatcag caaaaacaat aacccaatgc
    11881 gttttttccc tcgcatagat tcctctaatt aaattccaga acaccgaaat cattgtataa
    11941 ataaatacac aacgaattga ctacttgctt aattcggcac aacactgcct gaaataatcg
    12001 ctaaagaata tgatt
//
LOCUS       DAZKIP010000122        11932 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZKIP010000122 DAZKIP010000000
VERSION     DAZKIP010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.123"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            76..3444
                     /locus_tag="ACHOUK_003796"
                     /pseudo
     CDS             76..3444
                     /locus_tag="ACHOUK_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ECSE_1600 family autotransporter"
     gene            3447..4373
                     /gene="hipA"
                     /locus_tag="ACHOUK_003797"
                     /pseudo
     CDS             3447..4373
                     /gene="hipA"
                     /locus_tag="ACHOUK_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
     gene            4644..4913
                     /locus_tag="ACHOUK_003798"
                     /pseudo
     CDS             4644..4913
                     /locus_tag="ACHOUK_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            5347..5910
                     /gene="fimA"
                     /locus_tag="ACHOUK_003799"
     CDS             5347..5910
                     /gene="fimA"
                     /locus_tag="ACHOUK_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFP9899153.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
     gene            6271..6981
                     /locus_tag="ACHOUK_003800"
     CDS             6271..6981
                     /locus_tag="ACHOUK_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP9899154.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSNLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDIPHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            7023..9674
                     /locus_tag="ACHOUK_003801"
     CDS             7023..9674
                     /locus_tag="ACHOUK_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP9899155.1"
                     /translation="MTAFRAAFKAYRMHQVLLLPRFARLTIALSLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFITDDNNADLIP
                     CLSTDLLVSLGIKKSALLDNKEHSAEKHVPDNSACTPLQDRLVDASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPRFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADGTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDSNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGATFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYSTLNYRGAY
                     GNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGNDK
                     NSNCIVDYKLPEVSPGTLLNQQTAICR"
     gene            9688..10218
                     /gene="ydeS"
                     /locus_tag="ACHOUK_003802"
     CDS             9688..10218
                     /gene="ydeS"
                     /locus_tag="ACHOUK_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9899156.1"
                     /translation="MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            10231..10734
                     /gene="ydeR"
                     /locus_tag="ACHOUK_003803"
     CDS             10231..10734
                     /gene="ydeR"
                     /locus_tag="ACHOUK_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9899157.1"
                     /translation="MKRLHKRFLLATFCALLTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPAETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            10794..11708
                     /gene="ydeQ"
                     /locus_tag="ACHOUK_003804"
     CDS             10794..11708
                     /gene="ydeQ"
                     /locus_tag="ACHOUK_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9899158.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVNLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGTTTDSARQVFANTAPDATKASGVGVSLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
BASE COUNT         3338 a         2494 c         2890 g         3210 t
ORIGIN      
        1 tttaataaat ttcattttta atgtctgacg actcatctct ttcgtggctg tattgtttgt
       61 atttggagcg tttatatgaa tagaatctat cgcgtgatat ggaattgcac tctacaggta
      121 tttcaggcct gctcggaatt aactcgcagg gtaggtaaaa catcaacggt taatttgcga
      181 aagtcctctg gactgacaac gaaattcagt agattgacgc tgggtgtttt gctggcacta
      241 agcggttcag tgtctggtgc gagtctggaa gttgataatg gtcagattac caatattgat
      301 actgatgttg cttatgatgc ctacctggtt ggctggtatg gcactggagt gcttaatatt
      361 ttggctggcg gtaatgcctc cttaaccact attactacca gcgtcattgg cggtaatgag
      421 gattcggagg gtaccgttaa tgttttgggt ggcacctggc gattgtatga tagcggaaat
      481 aatgcaaggc ctttaaatgt gggtcaatcc ggaacgggga cgctgaatat taaacagaag
      541 ggtcacgtcg atggaggcta tttaagatta ggtactcaag ctgcaggcgt cgggacggtt
      601 aatgttgagg gagaggactc ggttttgacg accgaattat tcgaaatagg gagttacggt
      661 acgggctcat taaatattac ggataagggt tacgtcacga gttcaatagt cgccatttta
      721 ggatatcaag cgaacagtaa tggtaaggtt gtcgttgaaa agggtggcga gtggctaata
      781 aaaaataatg attcctcaat tgaatttcaa attggtaatc aaggaactgg ggaggcgact
      841 attcgcgagg gtgggctgat tacggctgaa aatacgatta tcggtggcaa tgccaccggt
      901 gtcggaaccc tgaatgtgca ggatcaagac tctgtcatca cggtacgcag actctataat
      961 ggatatttcg gtaatggcgc agtcaatatt tccaataatg gactaattaa taacaaagaa
     1021 tattcattgg tgggcgttca ggacggttcc cacggtgtcg tcaacgtgac cgataaaggg
     1081 cattggaatt tcctcggaac gggcgaagct ttccgctata tctatatcgg tgatgctggc
     1141 gacggtgaac ttaatgtctc gcgtgaaggt aaagtagatt cgggaattat cactgcgggg
     1201 atgaaagaaa caggcacagg caaccttact gttaaggata agaactccgt tatcactaat
     1261 ctcggaacta atcttggtta tgacggccac ggcgaaatga atatcagtaa tgaggggctt
     1321 gttgtcagca acggaggaag ctctctcggt tatggagaaa ccggcgtcgg gaaggttagc
     1381 atcaccacgg gggggatatg ggaggtcaat aagaatgtct ataccaccat tggtgttgcg
     1441 ggcgtcggaa acctcaatat tagcgatggc ggtaagttcg tatcgcaaaa tattactttt
     1501 ttgggcgata aagcaagcgg tatcggcaca ctgaacctga tggatgcgac atcgtcgttc
     1561 gatactgtgg gtatcaatgt cggtaatttt ggtagcggta tcgtaaatgt cagtaatggt
     1621 gccaccctta attcaacggg ctatggattt atcggaggaa atgcctccgg taagggaata
     1681 gttaatattt caacggatag tctctggaat ttaaagacgt cttctactaa cgcccaattg
     1741 ctacaggtcg gtgtattagg cacgggtgaa ctgaatatta ccaccggagg tatagttaaa
     1801 gcgcgtgata cacagatagc tctcaatgac aaaagtaagg gcgacgtgag ggtggatggg
     1861 cagaactctc ttcttgaaac attcaatatg tacgtaggga catctggtac gggtacgtta
     1921 accctgacga atagcggcac gctgaatgtc gaaggtggag aagtttactt aggtgttttt
     1981 gaacctgctg taggaacgct aaacattggt gcagctcacg gtgaggcggc ggcagatgcc
     2041 ggatttatca ccaacgcgac gaaagtagag tttggctctg gcgaaggtgt ttttgtcttt
     2101 aatcatacta ataacagtga tgccggctac caggtcgata tgctgattac aggtgacgat
     2161 aaagacggaa aagtgatcca tgatgcaggc catacggtgt tcaatgcagg gaatacttat
     2221 agcggtaaaa cgctggtcaa tgacggcctc ctgaccattg cgtctcatac ggcagatggg
     2281 gtaacgggca tggggtcgag tgaagtaacc attgcaagcc ccggtacgct cgacattctc
     2341 gcatcaacga acagtgcagg agattacacg ctgaccaatg cgctcaaagg cgatggcttg
     2401 atgcgagttc agctgtcatc ctccgacaag atgtttggct ttacacatgc aacagggact
     2461 gaattcgccg gtgttgccca actgaaagac agtaccttca ctctggaacg cgacaacacc
     2521 gctgcgctta ctcacgcgat gttgcagtct gacattgaaa ataccacatc ggtaaacgtg
     2581 ggagagcaat ccattggtgg actggccatg aatggcggta cgctcatttt cgatacggat
     2641 attcctgctg cgacgcttgc agagggatat atcagcgtcg atacgctggt tgtcggcgcg
     2701 agtgactaca cctggaaagg ccgtaactat caggtaaacg ggacgggcga cgtgcttatc
     2761 ggcgtgccta aaccgtggaa tgatcctatg gcgaataacc ctctgacgac gctcaatttg
     2821 ctggaacacg atgataacca tgtcggcgtt caactggtga aggcgcaaac ggttattggg
     2881 tcgggtggct cattaacgtt acgtgattta cagggcgacg aggtggaagc ggacaaaacg
     2941 ttacacattg cgcaaaacgg aacggtggtc gccgagggtg attatggatt ccgcctcacg
     3001 accgcaccag gtgatggttt gtacgttaac tatgggctga aagcgctgaa catccatgga
     3061 gggcaaaagc tgacgttagc cgaacatggc ggagcctatg gcgcaacggc cgatatgtcg
     3121 gcaaaaatcg gtggtgaagg ggatctggca atcaatacgg tgcgacaggt ttcgctttcc
     3181 aacggtcaga acgactatca gggggcaacc tacgttcaga tggggacatt acgtaccgat
     3241 gcggatggcg cgctgggcaa cacccgggaa ctgaacatca gcaacgcagc catcgtcgat
     3301 cttaatggat cgacgcagac ggtagagaca ttcaccgggc agatgggttc gactgttttg
     3361 ttcaaagaag ggtcgctgac ggtaaataaa ggtgggatca gtcagggtga actgacaggt
     3421 ggcggaaacc tgaatgttac aggggtaaag cagatatccc gctaggcatg attagagaag
     3481 aaaatgactt tcgcatctcg gttgctggcg cacaggagaa gacagcactg ctcagaatag
     3541 gcaatgactg gtgcattccg aaaggaataa cgccgacgac gcacatcatt aaattaccga
     3601 ttggggaaat caggcagccc aatgcgacgc tcgatctcag ccaaagcgtt gataatgagt
     3661 attactgtct gctgctggcg aaagaacttg ggttgaatgt cccggacgca gaaatcatta
     3721 aagcgggaaa tgtgcgcgcg ttagcggtcg aacgttttga caggcgttgg aatgctgagc
     3781 gaacggtttt acttcgcttg ccacaggagg atatgtgtca gacattcggt ttaccttcat
     3841 cggtgaaata tgaatcagat ggaggcccag gcatcgcgcg gatcatggct tttttgatgg
     3901 ggttcagcga ggcgcttcgc gatcgttatg attttatgaa attccaggtc ttccagtggt
     3961 tgattggcgc aacggatggc catgcaaaaa acttctccgt atttattcag gctggcggca
     4021 gttatcgact cacgccattt tacgacatca tttcagcatt tccggtcctt ggcggtacgg
     4081 gaatacacat cagcgatctc aaactggcaa tggggcttaa cgcatccaaa ggcaaaaaaa
     4141 cggcaatcga taaaatttat ccgcgacatt ttttggcgac agcaaaggtg ctgagattcc
     4201 cggaagtgca gatgcatgaa atcctgagtg actttgccag aatgattccg gcagcactgg
     4261 ataacgtgaa gacttcatta ccgacagatt ttccagagaa cgtggtgacg gcagttgaaa
     4321 ccaatgtgtt gaggttgcac ggtcggttaa gccgagaata cggtattaag taagagtaag
     4381 atatggggtt tggtcgttgt taatgagcag gacaatcatg accgccaacg actagattct
     4441 cccttggttt atattggtgc gcataatgta tattatgtta aatcgtatct gagtgatacg
     4501 aagtgatttt ccggtagtcg ttattcgttc aggattccag aacttaagct tctcctcttt
     4561 cataattggc ctgtgcatga cggatatagg ctggctggta tgccgatttg ccgtttgccg
     4621 catcttccca gcgttgaaga ctcaagctgg caaaccgcgt gcaaatgagc acgccctctt
     4681 tccatcaaca cttcaggcag cttgcgggaa tgagtccact ccggtatcag aaatggctac
     4741 gtctcaatga agtcaggcga acgatgctga atgaacatta cgatgtcacc acagcagcct
     4801 atgctgtcgg ttacgaaagc ttatcccatt tccgacggga atatttgcgg atgtttggag
     4861 agtcacctaa gagagatatt accaggttaa gaaagtctgt cggtcagctt taataaaccc
     4921 tgcacttatc tgtttagagc aattcggtgt tagttacacc aggcaaacat taactatagc
     4981 taatgattta tagccatata aaccattagt agcccttgac ctttcctgac acttcccttg
     5041 ttcgcaataa tattctgttt agtcacggtt tataaataac atgcataatg aaatcgtcat
     5101 gttatttagc gacataaaat gtttttgcaa atactctctt caacaagtgc agcaataaac
     5161 ttgttgtgat taggtgtcct tgtgatttaa agatattatc gttcttcgtg aaatgtaata
     5221 accctggaaa ctatattctc agggcaacaa ccaatcaaga atgattcttg ttttataagc
     5281 aataacagaa tgacttaatt gttaactctc tttattcagc tacttaaata taaattttgg
     5341 agaattatga aactcaaaca tgttggtatg attgtcgttt ctgtgttggc gatgtcgtct
     5401 gctgcggtaa gcgcagccga gggtgatgaa tcagtaacga ccactgttaa tggcggtgtt
     5461 attcatttta aaggtgaagt ggtaaatgcc gcttgtgcga ttgattccga atcaatgaac
     5521 caaacggttg agctgggtca ggttcgttct tctcgcctgg ctaaagcggg tgacctcagc
     5581 tccgccgttg gcttcaatat caagctgaat gattgtgata ccaatgtttc cagtaatgca
     5641 gctgttgcat tcctgggtac tactgtcacc agtaatgacg atacgttagc gctgcagagt
     5701 tcagcggcag gctctgccca aaatgtcggt attcaaattt tggaccgtac gggtgaggta
     5761 ttaatacttg atggggccac ttttagtgct aaaaccgact tgattgatgg cacgaatata
     5821 ctaccattcc aggctcgtta tattgctctc gggcagtccg tagctggtac tgcaaacgca
     5881 gatgcgacct tcaaagttca atatctataa ttcaatatta aggtggctat acggcatctg
     5941 aaattaaaac agggtgtgtg tggcatggct acgcacaccc gagcaattca ccaatgtgaa
     6001 tctcattatt aatgatgagt ggtttgtatt tggagcgcta tgaaaaatca cataatatct
     6061 aaacgttcaa taaacaacag tagttaaact tactgttatt aaaaatattt actttttagc
     6121 aaggttttcc tattaaaaga gaaccaacct ttattacaaa cgcattaagt ctggaaaccc
     6181 ggtgactcgc caatgtatta gcaacattta ccgattgtct gatgtaacgg ttttcccgct
     6241 tatcctctta acacatggag aatgcatcag atgcaaacta cgcgtacccc ctactctatt
     6301 tcctttatgg cgacagtgtt acttttattg ctctttgctt gccatagcac cgttgccaac
     6361 gcagctgtcg cactgggtgc gacgcgggta atttatccgg caaaccagaa acaggtttta
     6421 ttgcctgtca cgaataatga tcctgccagt gtctatctca ttcagtcatg gatagaaaat
     6481 gctggtgacc aaaaagacac ccagtttgtc attaccccac ctcttttttc catgcaaggt
     6541 aaaaaagaga atacgctgcg gataatcaac gccactaatc atcagttacc cggcgaccgt
     6601 gaaagcctgt tctgggttaa tgttaaagct attcctgcta tggaaaaaga ccagaagaat
     6661 gagaatacat tacagctggc aattattagc cgcataaaaa tgttttatcg gcccacgaac
     6721 ctggcaatgg cacctgaaga agcgcctgca atgcttcggt ttcgtcgcag cggtagcaat
     6781 ctcactctta ttaatccaac gccctacttc attaccgtaa caaatatgaa agcaggaaat
     6841 agcaatctcc caaacaccat ggtgccgcca aaaggtgaag tgtcggtgga tatcccgcat
     6901 gctgccaccg gggatatcag ttttcagacg atcaatgatt acggcgcatt aactccgcgt
     6961 attaaggcaa ccatgcagta agccagtgac ctaattaata ataaactcaa tcggaattaa
     7021 taatgacagc ctttcgtgcc gcatttaagg catatcgtat gcatcaggtg ttactactgc
     7081 ctcgctttgc acggcttacc attgccctta gcttagcaac tgcggtattt cccgttgacg
     7141 cagagtatta ttttaatcca cggtttttaa gcaatgatct tgctgaatct gtcgatttat
     7201 ccgcctttac taagggacgc gaggcaccac ctggtacata tcgggtcgac atttatttga
     7261 acgacgaatt tatgactagt cgggatatta cttttattac agatgataat aacgcagatc
     7321 tcatcccatg cctgagtaca gatcttcttg tcagtcttgg cataaaaaaa tctgcgttat
     7381 tagacaataa ggaacattca gcagaaaaac atgtgccgga caacagcgca tgcacaccac
     7441 ttcaggatcg tttggtcgac gcatcaactg agtttgatgt tggtcagcaa catctgtcac
     7501 tctccgttcc gcaaatttat gttggcagaa tggctcgcgg ctatgtttct cctgatctgt
     7561 gggaagaagg aataaatgct gggcttctaa actatagttt taatggtaat tctattaata
     7621 atcgtagtaa ccataatgca ggaaaatcca actatgcata tttgaattta cagagtggca
     7681 tcaacattgg tagttggcga ctacgcgata actcaacgtg gagttataac agtgggagta
     7741 gcaattcatc tgacagcaat aaatggcagc atatcaatac gtcggctgaa cgtgacatta
     7801 ttcccttacg ctcacggtta acggtaggtg atagttatac cgatggcgat atttttgata
     7861 gtgtgaactt tcgtggcctc aaaataaatt caacagaagc gatgttgccc gatagccaac
     7921 atggtttcgc tccggtgatt catggtattg cccgtggcac cgcacaagtg agtgtaaaac
     7981 agaacggata cgatgtttat cagactactg ttccacccgg cccttttact attgatgata
     8041 tcaactctgc ggccaatggt ggtgatctgc aagtaaccat aaaagaggca gacggcagta
     8101 ttcagacatt atatgttcct tattcgtctg ttccggttct ccaacgtgct ggatatacgc
     8161 gttatgcgct tgccatgggg gaatatcgta gtggaaataa cctgcaaagc tcccccaggt
     8221 tcatacaagg tagcttgatg catggactgg aaggaaactg gacaccttat ggcggaatgc
     8281 aaattgcaga agattatcag gccttcaacc ttggtattgg taaagattta ggactttttg
     8341 gtgccttttc tttcgatatc acgcaggcca atacgacact tgcagatggc acccgtcaca
     8401 gcgggcaatc ggttaaatcc gtctacagca aatccttcta ccagacagga accaatatcc
     8461 aggtcgcagg atatcgctat tctacgcaag gtttttataa cttatccgac agtgcctaca
     8521 gtcgaatgag tggttacacc gtcaagcctc ctaccggaga cagcaatgag cagacacaat
     8581 ttattgatta ttttaatctg ttctacagta agcgtggtca ggaacaaata agcatctctc
     8641 agcagcttgg aaattacggt gcgacatttt tcagtgccag tcgccaaagt tactggaaca
     8701 cgtcacgcag cgaccagcaa atatcatttg gattaaatgt gccgtttggt gatattacga
     8761 cttcgctgaa ttacagctat tccaataata tatggcaaaa cgatcgggat catttactcg
     8821 cttttacgct taatgttccc ttcagtcatt ggatgcgtac agacagtcag tcggcatttc
     8881 gtaattcaaa cgccagttac agtatgtcaa acgatttgaa aggcggcatg accaatctat
     8941 cgggggttta tggcactctg ctgccggata ataacctgaa ttatagcgtt caggtcggta
     9001 acacccacgg aggtaataca tcgtctggca ccagtggtta cagtactctt aattatcgtg
     9061 gagcttacgg caatactaat gtcggttaca gtcggagtgg tgacagcagc cagatttatt
     9121 acggaatgag tggtgggatt attgctcatg ctgatggcat cacctttgga cagccgctgg
     9181 gcgacacaat ggttctggtt aaggctcctg gcgctgataa tgtcaaaata gagaaccaga
     9241 ccggaattca taccgactgg cgtggctatg ccatattacc atttgcgaca gaatatagag
     9301 aaaatcgtgt cgctcttaac gcgaattccc ttgcagataa tgttgaactg gatgaaaccg
     9361 tggtcactgt catcccaact cacggtgcta ttgccagagc aacatttaat gcacaaatcg
     9421 gcgggaaagt attaatgacg ttgaagtacg gtaataaaag cgttccattc ggtgcaattg
     9481 tcactcacgg agagaataaa aatggcagca ttgtcgcgga aaacggtcag gtttatctga
     9541 ctggacttcc acagtcaggg aaattacagg tttcatgggg caatgataaa aactcaaact
     9601 gtattgtcga ttacaagctt cctgaagtct ctcctggaac cttgctgaac cagcagacag
     9661 caatctgtcg ctaaggaaca atagattatg aaatacaata acattatttt cctcggttta
     9721 tgtctggggt taaccaccta ttctgcttta tccgcagata gcgttattaa aattagcggg
     9781 cgcgtcctcg attatggctg cacagtctca tcggattcgc ttaattttac cgtagatctc
     9841 caaaaaaaca gtgccagaca atttccaacg accggtagca caagtccagc cgtccctttt
     9901 cagattacgt taagtgaatg cagcaaaggg acaacggggg ttcgggttgc atttaacggt
     9961 attgaggacg cagaaaataa tactctgttg aaactggatg agggaagcaa tacggcctcc
    10021 ggtttaggta tagaaatact ggacggaaat atgcgtccgg tgaaactgaa tgaccttcat
    10081 gccgggatgc agtggatccc actggtacca gaacagaaca atattttgcc ttactccgct
    10141 cgtctgaagt caactcagaa gtccgtcaat ccgggactgg tgagggcttc ggcaaccttt
    10201 acccttgaat ttcaataatc agagtcagat atgaaaagac ttcacaagag gttcctctta
    10261 gctacgtttt gcgcgttatt aacagcaact ctccaggccg ccgatgtcac tatcactgtt
    10321 aatggtcggg tagtcgctaa accctgcact attcaaacca aagaagctaa cgttaatctc
    10381 ggggatcttt atacgcgcaa tctgcaacaa cctggttctg catctggctg gcacaatatt
    10441 actttgtcat taaccgattg tccggctgaa acaagtgcag tgacggcaat cgtgacaggt
    10501 tcaactgaca atacgggtta ttacaaaaat gaaggtactg ccgaaaatat tcagatagag
    10561 cttagggatg accaggatgc gacgttaaaa aatggcgata gcaaaacggt tattgttgat
    10621 gagatcactc gtaatgcaca gtttccactt aaggcaagag ctatcacggt gaatggaaac
    10681 gcaagccagg gaacgatcga ggcgctaatc aatgtgatct acacctggca ataacgtcga
    10741 ttcgattttc ttagccttct ttttaaatca attttttaac cttaggatat atcatgggta
    10801 aaacaatcag tataaaggtc ctgttcggta tttatcttct attaatggca ggcaaagttt
    10861 ttgccttcag ttgtaatgtc gatggcgggt caagtattgg agctggtaca acctctgttt
    10921 atgttaatct cgaccctgta atacagccgg gccagaatct ggttgtagac ttgtctcagc
    10981 atataagttg ctggaatgat tacggcggct ggtacgacac tgatcatata aacctggtac
    11041 aaggctcagc tttcgcaggc tctctacagt catataaagg atcgctgtac tggaataatg
    11101 tgacctaccc cttcccgttg acgacaaata ccaacgtatt ggatattggt gataagacac
    11161 cgatgccatt accgttaaaa ctctatatta ccccagtcgg ggcggccggt ggagttgtca
    11221 taaaagccgg ggaagttatt gcccgtattc atatgtataa aattgccacg ttaggaagcg
    11281 gaaatccgcg taattttacg tggaatatta tttcaaataa cagtgttgtt atgccaacag
    11341 gcggctgcac tgttgatagt cgtaatgtca ccgtgaatct cccggacttt cctggaagcg
    11401 cagaaatccc ccttggcgta tattgctcaa gcgaacaaaa gctctcattt tatctttcgg
    11461 gtactaccac tgacagtgct agacaagtgt ttgctaacac agcaccagat gcaacaaaag
    11521 catcgggagt gggtgtctct ctgatgcgta atggaaaaat actggctacg ggagaaaatg
    11581 tctccctggg gacagtaaat aaaagcaaag tacctctggg tttaagcgca acctatggac
    11641 aaacagggaa caaagtttca gccggaaccg tccagtcagt cattggtgtc acttttatct
    11701 atgaataatt gtgattttga ccttagtcta tgcgctccat tcacgaaaat tgcgcggata
    11761 attattttgt gaaggctatt atcctacact tgtaagaaaa tccgcgcaca attgaagcca
    11821 gagtcttatt ttttatattg aaaaatatgt taatttaaac actatgagag atgatgctta
    11881 ccttatcgta tttaatctat tgtttaacga taatagatta aaaatagaaa ga
//
LOCUS       DAZKIP010000123        11811 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZKIP010000123 DAZKIP010000000
VERSION     DAZKIP010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.124"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            96..746
                     /gene="csgD"
                     /locus_tag="ACHOUK_003805"
     CDS             96..746
                     /gene="csgD"
                     /locus_tag="ACHOUK_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFP9899159.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            751..1140
                     /gene="csgE"
                     /locus_tag="ACHOUK_003806"
     CDS             751..1140
                     /gene="csgE"
                     /locus_tag="ACHOUK_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFP9899160.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1165..1581
                     /gene="csgF"
                     /locus_tag="ACHOUK_003807"
     CDS             1165..1581
                     /gene="csgF"
                     /locus_tag="ACHOUK_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFP9899161.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1608..2441
                     /gene="csgG"
                     /locus_tag="ACHOUK_003808"
     CDS             1608..2441
                     /gene="csgG"
                     /locus_tag="ACHOUK_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFP9899162.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2505..2996)
                     /gene="ycdZ"
                     /locus_tag="ACHOUK_003809"
     CDS             complement(2505..2996)
                     /gene="ycdZ"
                     /locus_tag="ACHOUK_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFP9899163.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILSYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLIFGYAMKNSGLWLAARSAKTALREQEIK
                     NKA"
     gene            complement(3098..3652)
                     /gene="ycdY"
                     /locus_tag="ACHOUK_003810"
     CDS             complement(3098..3652)
                     /gene="ycdY"
                     /locus_tag="ACHOUK_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFP9899164.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3676..4413)
                     /gene="ycdX"
                     /locus_tag="ACHOUK_003811"
     CDS             complement(3676..4413)
                     /gene="ycdX"
                     /locus_tag="ACHOUK_003811"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFP9899165.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4468..5406)
                     /gene="ghrA"
                     /locus_tag="ACHOUK_003812"
     CDS             complement(4468..5406)
                     /gene="ghrA"
                     /locus_tag="ACHOUK_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFP9899166.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALA
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLKPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGWEELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5640..5727
                     /locus_tag="ACHOUK_003813"
     tRNA            5640..5727
                     /locus_tag="ACHOUK_003813"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5674..5676,aa:Ser,seq:gga)
     gene            complement(5877..6719)
                     /locus_tag="ACHOUK_003814"
     CDS             complement(5877..6719)
                     /locus_tag="ACHOUK_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFP9899167.1"
                     /translation="MSDITISRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISVLTESIGGKTAPDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDTQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLN
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     GNITLRLDDHIQSVQGKESYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPD
                     MLAV"
     gene            complement(6804..7001)
                     /locus_tag="ACHOUK_003815"
     CDS             complement(6804..7001)
                     /locus_tag="ACHOUK_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFP9899168.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(7013..7504)
                     /locus_tag="ACHOUK_003816"
     CDS             complement(7013..7504)
                     /locus_tag="ACHOUK_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFP9899169.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDVDGIELAELINMVGDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGNGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(7501..7875)
                     /locus_tag="ACHOUK_003817"
     CDS             complement(7501..7875)
                     /locus_tag="ACHOUK_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFP9899170.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(7922..8296)
                     /locus_tag="ACHOUK_003818"
     CDS             complement(7922..8296)
                     /locus_tag="ACHOUK_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFP9899171.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLNAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(8459..8680)
                     /locus_tag="ACHOUK_003819"
     CDS             complement(8459..8680)
                     /locus_tag="ACHOUK_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFP9899172.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(8743..9219)
                     /locus_tag="ACHOUK_003820"
     CDS             complement(8743..9219)
                     /locus_tag="ACHOUK_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFP9899173.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(9235..9720)
                     /locus_tag="ACHOUK_003821"
     CDS             complement(9235..9720)
                     /locus_tag="ACHOUK_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFP9899174.1"
                     /translation="MTTVSHNSTTPSVSVTTASGNNPPQLVATPVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(9811..10629)
                     /locus_tag="ACHOUK_003822"
     CDS             complement(9811..10629)
                     /locus_tag="ACHOUK_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFP9899175.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMRHVPSIFGENRHTSRSEHY
                     AYIPTITVLENLQQEGFQPFFACQTRVRDQSRREYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            complement(10969..>11811)
                     /locus_tag="ACHOUK_003823"
     CDS             complement(10969..>11811)
                     /locus_tag="ACHOUK_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFP9899176.1"
                     /translation="LSVNRLVYFSLLKKLFQAMNLCQIPGRAGALLTTLLRHISTING
                     FDNLMAQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYPTEGGMQDIIDCIRAELGV
                     GTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQGTMFGDGGMGGWRNMQGNT
                     PVTPLVDAGCNMVIVTHLSDGSLWDRQAFPDTTILEIRPRKRLKYAGDGGNSGGLLSF
                     TSAHTDAWRQQGYEDTMLAMEHIRKPLAARQALTRSEAVLQKSLDITEEADLALRNAM
                     ARIK"
BASE COUNT         2965 a         2990 c         2942 g         2914 t
ORIGIN      
        1 acacacagca gtgcaacatc tgtcagtact tctggtgctt ctattttaga ggcagctgtc
       61 aggtgtgcga tcaataaaaa aagcggggtt tcatcatgtt taatgaagtc catagtattc
      121 atggtcatac attattgttg atcactaaac cttctttgca ggcgacagct ctcttgcagc
      181 accttaaaca atcgctggca attacaggaa aattacataa tattcaacgt tctctggacg
      241 atatctcttc aggctctatt attcttctgg atatgatgga agcggataaa aagcttatcc
      301 attattggca ggatactttg agcaggaaaa acaacaatat caaaatattg ttgctaaata
      361 cgcctgaaga ttacccgtac cgcgacattg aaaactggcc tcatatcaac ggcgtttttt
      421 atgccatgga ggatcaagaa cgcgttgtca atggattgca aggcgtcctg cgcggcgaat
      481 gctactttac gcaaaagctt gccagttacc tgattacaca ttctggtaac tatcgttata
      541 acagcacgga atcagccctc cttactcatc gggaaaaaga gatcctgaat aagctgcgta
      601 tcggcgcgtc taataacgag atcgctcgtt cgttgttcat cagcgaaaat acggttaaaa
      661 cgcatcttta taatcttttc aagaagatag ccgtaaaaaa ccggacacaa gcggtttcct
      721 gggcaaacga taacctcagg cgataaagcc atgaaacgtt atttacgctg gattgtggcg
      781 gcagaatttc tgttcgccgc agggaatctt cacgccgttg aggtagaagt cccgggattg
      841 ttaactgacc atactgtttc atctattggc catgattttt accgagcctt tagtgataaa
      901 tgggaaagtg actatacggg taacttaacg attaatgaaa ggcccagtgc acgatgggga
      961 agctggatca caataacggt caatcaggac gttattttcc agactttttt attcccgttg
     1021 aaaagagact tcgaaaaaac tgtcgtcttt gcactgattc aaactgaaga agcactaaat
     1081 cgtcgccaga taaatcaggc gttattaagt acgggcgatt tggcgcatga tgaattctaa
     1141 ataaaaaatt gttcggaggc tgcaatgcgt gtcaaacatg cagtagttct actcatgctt
     1201 atttcgccat taagttgggc tggaaccatg actttccagt tccgtaatcc aaactttggt
     1261 ggtaacccaa ataatggcgc ttttttatta aatagcgctc aggcccaaaa ctcttataaa
     1321 gatccgagct ataacgatga ctttggtatt gaaacaccct cagcgttaga taactttact
     1381 caggccatcc agtcacaaat tttaggtggg ctactgtcga atattaatac cggtaaaccg
     1441 ggccgcatgg tgaccaacga ttatattgtt gatattgcta accgcgatgg tcaattgcag
     1501 ttgaacgtga cagatcgtaa aaccggacaa acctcgacca tccaggtttc gggtttacaa
     1561 aataactcaa ccgattttta agccccagct tcataaggaa aataatcatg cagcgcttat
     1621 ttcttttggt tgccgtcatg ttactgagcg gatgcttaac cgccccgcct aaagaagccg
     1681 ccagaccgac attaatgcct cgtgctcaga gctacaaaga tttgacccat ctgccagcgc
     1741 cgacgggtaa gatctttgtt tcggtataca acattcagga cgaaaccggg caatttaaac
     1801 cctacccggc aagtaacttc tccactgctg ttccgcaaag cgccacggcg atgctggtca
     1861 cggcattgaa agattctcgc tggtttatac cgctggagcg ccagggctta caaaacctgc
     1921 ttaatgagcg caagattatt cgtgcggcac aagaaaacgg cacggttgcc attaataacc
     1981 gaatcccgct gcaatcttta actgcggcaa atatcatggt tgaaggttcg attatcggtt
     2041 atgaaagcaa tgtcaaatct ggcggggttg gggcaagata ttttgggatc ggtgccgaca
     2101 cgcaatacca gctcgatcag attgccgtga acctgcgcgt cgtcaatgtg agtaccggtg
     2161 agatcctctc ctcggtgaac accagtaaga cgatactttc ctatgaagtt caggccgggg
     2221 ttttccgctt tattgactac cagcgcttgc tggaagggga agtgggttac acctcgaacg
     2281 aacccgttat gctgtgcctg atgtcggcta tcgaaacagg ggtcattttc ctgattaatg
     2341 atggtattga ccgtggtctg tgggatttgc agaacaaagc agaacggcag aatgacattc
     2401 tggtgaaata ccgccatatg tcggttccac cggaatcctg acagataaaa atgccggaat
     2461 taataatccg gcatttactg tttaagcgaa agcgtgagtg cccctcacgc tttatttttg
     2521 atttcctgct cacggagagc cgtctttgca ctacgcgccg ccagccacag gccgctgttt
     2581 ttcattgcgt aaccaaatat cagcccaagc gccagcgaag gtaacaccag tttccagtca
     2641 ccctgcccgg caaacgtcgc gcatgcgcct ataaacgtcc ccggaacaaa tgaaagcagc
     2701 agctgtttgg cctgaataca catcagaaac gcgacaatac cggttatgac ataactgaga
     2761 atttccagat gtggcgccag tgcactaccg taaataatga ccatcgccca caccacgcca
     2821 ctaagcagag ttgcagcgga gatcgccagc cctttcagcc caccttgcgg gcaggcaaag
     2881 taggccgtac agcccaggaa gcccgcccag cttagtaagc ccagggaaac agccacccat
     2941 ccccagatac cggagagaat gcctgttgtg attgcaatag agagaagtat gttcatgccg
     3001 cgcatgatag cagaaaacgc ggccatgaat gagatcgagc acacatttta tgcaacagca
     3061 aattatctgt tgcagttatg agtgacgcat atcacattta ctcttcagaa tcttcttcca
     3121 gctcgtccca cattgcacta atggcatcgc gggttagcgg tgccatggtg cgccagaaag
     3181 gcgtggttgc atgggcctcc actttgccaa ggaacgcacc acaccagggt aacagatact
     3241 cactgaacag tgtttccagt gcttcgctct catcttccgt tgactgatct tccagccagg
     3301 aagccgcaag cagcaatgtg ccgatgtgat cggcaggcgt atccgctaat ggcatccctc
     3361 gttcagaaag aaaagcgcgc acttccgctt ccgttgcgtc ctcaacccat gcgctacgat
     3421 atggcggcac ggcacattca tcgccgataa acaacgcatt gtaatcggca gagacttgcg
     3481 tcatatcaca gcttttctgc aaacgtgtca gcaactcatc ctgctccagt ggccagttcg
     3541 cagccagttt cccctcacga atcagggtaa acagcggcac cagtaaagga tcttgcggtt
     3601 ggcggtaata gagcgaaccc agcacacgac agaggataga aaactcgttc attaatatat
     3661 ttccattact agtaattaaa ggtctgcaaa ttccgcaatc ggtgccatac cgcgagattc
     3721 aaggaagttc agtaagcggc gcggagaaac attcaaaatg cgctctggcg gaaaatctac
     3781 cgcgtcgagg attttaagac actcttcaaa ttcccccatg gtaaacgcgg tgtgagaatc
     3841 cgagcctaat gccacccaac ctccagcatc acgtaccgct gcggctaccg cacgacagtt
     3901 gtcttcactg cccttacgtg agtgtaaaaa tgaggaatta ttgatttcca gcgccacctg
     3961 atgtttcgcg gctgcttcag caacggcttt aacatctatc tcatatttcg gatttccggg
     4021 atggcttatt atatgcacat tgccgctggc gatagtggcg atcatcgctt gtgtattggt
     4081 cgctttgtca tgtggtgcaa aaaccggctc atgaaaaccg gcaataatca gatccagcga
     4141 gtcaaacatt ttaccgctgc aatcaatttc accatcaaca tttttaatgt tagcttcgat
     4201 gccgcgcagg atccctaccc catcaaccac tcgcggccag atacgcatgt taatgaagtg
     4261 ccagtgatgc ggcgcatctt ccatgtccgg gccatgatcg gtaatcgcaa aaagtttaat
     4321 gcccttttgt ttggcctggg caatgtaatc actcaatgtg ctatatgcat gtgtgctggc
     4381 aacggtatgc atatgaaggt cgacgggata cataactctc tcctgtattc aattcccgcc
     4441 aaggatagca ggaatcctga tgctttatta gtagccgcgt gcgcggtcga cttgcccgca
     4501 gaccctctcc cctttttcga gctgggcaat agtgcgagaa atgtactcca cagcttcagc
     4561 gggacgggta atcgcggcga catgtggtgt tatcgtcacg cgtggatgtt gccagagcgg
     4621 actttcaggc ggtaagggtt cacgattaaa aacatccaac attgcgcctt taactttgcc
     4681 gctatccagc gccgcgagca ggtcatcttc cacgacatga acaccacgcg ccaggttgag
     4741 gagatacgcg ccatccggta atttttcgag taattgttga ttaataatgc cgacggtttc
     4801 aggggtattc ggtaacaaat taatcaatac ccgacattgg ctcagaaatg cagacagttc
     4861 ttcccatccg gcaaagcttt gcacgccagg ccacgattta cgggttcgac tccagcaacg
     4921 cagcggaaag cgccaggttt gcagactctg agcaacttta ctgcccagta cgcctgcgcc
     4981 caaaatgccg atggtaaaat cttcccgatg atattcaggc agcggttgcc aatgcgaact
     5041 attttgctgg atgcgataat cgtcaaaacg tcgaaaccaa tgcagcacct gactgacagc
     5101 atattcctgc atttgctcgc ccataccggt atcttccagg cgaaaaagtg gaacagaagg
     5161 cttcagcatt tcagggtgtg cctgtagctt gctcaaaata gaatcaacac cggccccgag
     5221 tgcgaacacc gctttaagat cgcgccctgc cagcatttca acaggaggat gccaggctaa
     5281 agcataatca gcagagtcat tatctccgct tttccatgct ctgactcttg cctgaggaat
     5341 agctttgcgc agtgcctcta tccaccattg ggtatcgaac gttgggtgat aaaagatgat
     5401 atccatactg actcccgaaa agcgttgtgc gaaatttatt cgcacttatc gttatgatct
     5461 acaaagacca ccagcataac aaatccgtgg tcggtggcca aaaaagcaga ttttgcttat
     5521 taaaactgca tattgattga agtttgaata aacgcgcgat tttttaaaaa agtttgttga
     5581 cctcaggtca tgatttccct aaattagcgc ccgttccagc aagacaggaa cgacaatttg
     5641 gtgaggtgtc cgagtggctg aaggagcacg cctggaaagt gtgtatacgg caacgtatcg
     5701 ggggttcgaa tccccccctc accgccagat tataagcgca tgttattgtt taacatgcgc
     5761 ttttttattt tcccttccag acagtatgcc atcgggtatg ccatctgaaa ccggtttata
     5821 tgccccgcga tcatatctta tcggacaatt tccgatcttt gagcatactg gcaaagctat
     5881 actgccagca tatcaggata gtgttttgcg ataatatcat tcagtctgtc aaccagttct
     5941 ggttttctga acgtgatatg cgcggagccc ttcttaaagt atctgatgct gaacatctca
     6001 tcttcatagc tctctttacc ctgaacagac tgaatgtgat catccagacg aagggtgatg
     6061 tttccccggt tgtcggggat tggtttaccg ctgaacaggt gcaacattct ttccagatcg
     6121 gcgagtcggt cagcctgttg tccggtgata agatgaaatc cccaccggtc ccatctcacc
     6181 agattgttga cgataatttt actgccaaat ttacaggggc aattggtttt gtaattccag
     6241 ttcagccctc tgaagacgtt gatcacccct cgctcaaaca cctcatcctt attctggtgc
     6301 agttgttcaa atgtgctcag aatgttcgct tcgctgattt ccggaaaatt atcgtactcc
     6361 agtgaccggt accatgtatc acgggcctgc gtatccatta atgacagcat cccggaacgt
     6421 tgcatcaggt cacgccagat ttcgcgatca agattccggg taatggcttt cattgctgtt
     6481 tcgggtttct ccatcagcca gcagccgcag cgaaaatcct gtttcatggc ccagtccggg
     6541 gcagttttac ctccgatgct ttccgtcagc accgagattt cagccagttg tcggataagt
     6601 gtgtcgattt gcaacagtgt actctttcgt acagccagaa tgtggcgaat gctggttgag
     6661 cagataacgt ccgtatgccc gttgaccact tccggacggg agattgtgat atctgacata
     6721 atcgatattc ctttttgcag ataaatgaaa acgctagccc tgtgcaggct ggcgtcagag
     6781 gaatgaagtc tggtgagtga gcttcaccgg ttctggtgca gaagctgcag atgacgcagg
     6841 gtacggacat cctcccgggc ctgttcgata cagggcaaca gtgctgctga atccgtttta
     6901 tcctcatcgt tgtcaaagat aattcccgat ccgcagtcga tattgtcctg cagccacgca
     6961 atcagaatat ccagcgctgt ttccgtggtt aatgatttca tgttgttcct ggttaccagt
     7021 cgaaagtggg taaacccggc aggcattcag cgctggcatc cagatgaatg agactgacgc
     7081 cataacgccg gatgagtgtg acgaccagac gacggaacgt ttttgacaga cccaggcgtt
     7141 taagccgcag aaccgggtac gaccacgcat cggtacgcaa cagatagccg ttaccggtaa
     7201 aatgaatcca ttctgattca ccgaagtcac tggtctggtg tgacagcgag tacagccagg
     7261 cgttgtccgt ttccgtgata tgtgcggtac tgcagcgtat gccggtaagg gtagcaaatg
     7321 gtggcgtgca ttctgctgag gtctggtcag attcatcaac aacacgaagg gagtagccgt
     7381 tatcgccgac catgttaatc agttcagcga gctcaatacc atcgacgtca acgacaatgc
     7441 gccccatgtt cagtgcctgt acgttaacgc tgtcggcttc cagtgtcagg gccagtttca
     7501 tcatttcgcc tccggatact tacccagggt aatgttattt accgttctgt aattgtcgcg
     7561 ggtcatcagg ccggtcgccc ggcgagcccg gaggatatca atactgttta ttaactgaga
     7621 gcggggacag gcgctgaatc ccggctggtc ggtacgcacc agcgcgtatt tttccacgag
     7681 aaaattaact gcatcacaca gtgaaatgcc tgcctcaata tgctgctcaa tcacacgttc
     7741 atcggcaaac ggtgtgtcat tcagtgtgag gccataatgc tggtccagca gtcggatgag
     7801 cagtgtctgc cagatggtga cgggagacgg gcagcgagat acctcccgta catgtgtgtc
     7861 ggataatgtt ttcatcttaa gttctctgta actggtagtg ctaaaggtga gagcttcggg
     7921 gttatgaggt ggttgcgggt gctggtgtcg gataaacagc cagataaacg taaccacagg
     7981 agccgagggt gtcggcttcg caggtcagcc cttttgcatt cagcgtgacg gtatgctgat
     8041 atcggggatt cagttcaccg ctggtgagca tgagttccag ttgtttcatc agcagcggaa
     8101 aggcctggtc cagatggtat gcatctgcgt cgctgaagcg gcctctgata ccggcgcggt
     8161 cggcaaggta atgcaggcgg ttaccctctt gcaccagacg agcgccaaaa cagggcctca
     8221 ctgtgcaggg aagcccccac caggggcggt cgtgattgtc atcgggatga gttatcccgg
     8281 agagtttgtc tgacacgata aaatcctctc tgaaaagtgg ttaagaatgg tttaacgatg
     8341 acaggtcagc cggcgcgcag accggtttcg gtgaagatgt atgcattgat gagaatggcc
     8401 tcatccacag cggcgtctga ggtcatctgg tcgtactcgt ttcccgggtg acggaacatc
     8461 agttcagggt gacactcatc agccggacat acgggccaaa actgtcctta cggcgttctg
     8521 caaacacggc aagaacgccg ggaatatcct gcacttcacg accagtatac gcttcagcac
     8581 tgccgtgcca gcgatactta ccggtacaga acggaaaaag acgggatgca ggatgctgtt
     8641 ggtgaatacg catggcttca ccacgggtga tgattctcat gatgtgatac ctctgaagac
     8701 agaagataaa agtgaaaaca ggtgtgatgt ggttgtgacg ggttaaagca gaccatgttc
     8761 ggcaaaggag aaaacctggt tgccaccgac tatcagatgg tccggcaccc ggatatccac
     8821 caggcccagt gcctgtacca gacgttcggt gataagccgg tctgccttac tgggtgtgac
     8881 ttcaccggac gggtgattgt gcgccagcac cacggcagcg gcattgtggt acagggcgcg
     8941 tttaatcact tcccggggat gaacttccgt acggttgatg gtgccggtga agagggtttc
     9001 accggcaatc agctgattct ggttgttcag atacagcacc cggaactctt cccgctccag
     9061 tcccgccatg ttcagaatca gccattcccg tgccgcacgg gtggaggtga aggccacgcc
     9121 gggttcatga agatggcggt ccagggtttg cagggcccgc agaatgagac tgcgctcgcc
     9181 gggcgtcatc tctccgggca gaaaggaaag ctgctgcatt gtccctgcct ccattcagtc
     9241 aatgatgcgc ataatggcgc tgcattccgg atgctgcagg gcgtaatccc gcagccggta
     9301 atagtggacc gtcatggcat aacactccgt acgacaggca tgatgactgt acgtcatcag
     9361 acaggcagca ataccggcgg cttccgggct catttcagcg cggttgccgt tcatggcatt
     9421 gaacagtacc catgtttcgt catcatcgtc atccggttcg ggtgccataa atgccccgcc
     9481 gttgttcagg gtgtacagat tccagatacc accgcagtag tcttcgcaaa gacggtccat
     9541 ccagccgaag acacggggct ccagggtcac ccactgtgga atgaggccaa aatgctgcgg
     9601 ccagaagctg atgcgctgtt catcagggac gggagtggca accagctgag gtgggttatt
     9661 ccctgatgca gtggttacgg aaacagaagg tgtggtggaa ttatgcgaaa cggttgtcat
     9721 gaggttattc cttataaaaa gtaaatgaat ggaagaagcc ccggggcagg aacagatgtg
     9781 agtcaggact gcactttcag tgaaacggca tcagcgcatg ctctccagca gcgtttctgc
     9841 catcacccac aatgcgcgat tgagcttaat gtcggtatca atgctgtgaa tggcccgggt
     9901 atggatacgt tttccttttg cactgcgacc ggaaatcccg cctttcagca tattctcctg
     9961 gatggtctga taagcactcc acaggtcctt accgtaatcc tcccggcgtc gtggtgtcag
    10021 aatgtcggcg gtggtgacgg gctgatgttc gtcaccataa cggtaagtca gtgccgcctg
    10081 tgccagcgcc tggcgtgccg gtggcggcag gaccagcgac tgcatggcat cacgcttctc
    10141 ctctatccgg tcaaaaacgc ccaccacctc gtaagcccct tcaatgactc tgtccaccac
    10201 atttccccgg tgtggaacac gcaattctcc cagagactga ccgcagacca gtccattggt
    10261 acaaatggca cgaaaatatc cgggtaacat ctggtagctg gatgaaccgt catgggagtt
    10321 gagcagaata atttcaggga catgctgacc ggttatctgt ccggcccgcc gcagacgcag
    10381 catgtgtttt gtgtattccc ggcggctctg gtcgcgcacg cgggtctggc aggcaaagaa
    10441 cggctgaaag ccttcctgct gcagattttc caggacggtg atagtgggaa tgtacgcata
    10501 gtgttcactg cgagaggtat gtcggttttc cccaaaaata ctcggtacat ggcgcatcag
    10561 ttcttcgtgt gtcagcggac gatcacgacg tatctggttc gcataaccaa aacgactggc
    10621 taatctcatg atttgctcct tatgaatgat taagcgatat aaatgaaaaa gccgcgtctc
    10681 ccggagaaga cgcggcctga tgggtgaaat gaatgaggtt tattgtctga gaagcccttc
    10741 actggcgaac cacgtattaa gctgtattcc ggcatcgcca gtgcaactga ccttcagcat
    10801 tgcggataac ccgccggaaa tatgttcact ggttgttttt gtatcttccc ccaaaattaa
    10861 ataaattcat cttaaccatc acccagataa tatatatctg aaaatatttt caataacacc
    10921 agacaggaaa aacaccacca caggcggtgg cgtttctttg gggcgtcttt atttaatccg
    10981 ggccatcgcg tttctcagtg ccaaatctgc ctcttccgtt atatccaggc ttttctgcaa
    11041 taccgcctct gaccgggtca gtgcctgacg tgctgccagc ggtttccgga tatgctccat
    11101 cgccagcatc gtgtcttcat agccctgctg acgccaggcg tcggtatgtg ccgatgtaaa
    11161 actgagcaga ccgccgctgt tgccaccatc acctgcatat ttcagccttt tccggggacg
    11221 gatctcaagg attgtggtgt ccggaaaagc ctggcgatcc cataaagaac cgtcactcag
    11281 atgcgtcaca atcaccatat tgcatccggc atcgaccaga ggcgtcacag gggtatttcc
    11341 ctgcatattt cgccatcctc ccataccacc atcaccgaac attgtcccct gaacctcacg
    11401 gggacggaac agcaggggca gcgcggctga cgcaagcaga gcctctttct gctgtccgcg
    11461 gggcaggctc tggatatgct gaaaaacggc gtttttcgtg gttccgacac ccagttcagc
    11521 acgaatgcag tcaataatat cctgcatgcc cccttctgtg gggtacagcg acacatacag
    11581 cggtagcccg tctgccagag catcagtatc aagataatga tccatcagcg ctgtcagggg
    11641 ctcatctgac aacaacggct gagccatcag attgtcaaac ccgttgattg tcgatatatg
    11701 gcgaagaagc gtcgtaagca gtgctcctgc acgtccgggg atctggcaga ggttcattgc
    11761 ctggaacaat tttttcagca atgaaaagta aaccaatctg tttaccgaca g
//
LOCUS       DAZKIP010000124        11750 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZKIP010000124 DAZKIP010000000
VERSION     DAZKIP010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.125"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(120..476)
                     /locus_tag="ACHOUK_003824"
     CDS             complement(120..476)
                     /locus_tag="ACHOUK_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899177.1"
                     /translation="MNKNLILAFALFSLPVFAEEDLGPGKYVCDIRISSLDTATQILS
                     KSATVLDNGNNFIVQMPNGDQLYSPDLENVDDGIKQKATIGGVTFIRRPTFNDRFIVE
                     DGNTGFFYKMRNCEKK"
     gene            complement(684..1244)
                     /locus_tag="ACHOUK_003825"
                     /pseudo
     CDS             complement(684..1244)
                     /locus_tag="ACHOUK_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195265.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            1810..6063
                     /gene="fdeC"
                     /locus_tag="ACHOUK_003826"
     CDS             1810..6063
                     /gene="fdeC"
                     /locus_tag="ACHOUK_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
                     /protein_id="HFP9899178.1"
                     /translation="MSRYKTGHKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMGNTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDIEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVTYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLAKQLDTDSIRE
                     RRVLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNTSKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQKPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRSLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     FVPQDTNGVTVGAISEIKPGVYSAAVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTVKDAYDNPVTSLTPEAPSLAGAAAEGSTASGW
                     TNNGDGTWTAQITLGSTAGELEVMPKLNGQNAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLALSASLTGTASEGATVSSWTEKGNGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKTTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGNWTEKGNGVYVSTLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVNNQLANGQSANQITLTVVDTYGNPL
                     QGQEVTLTLPQGVTSKTGNTVTTNAAGKADIELMSTVAGEHNISASVNGAQKTVTVKF
                     NADASTGQANLQVDAAAQKVANGKDAFTLTANVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANDEGKAELQVVSVTAGTYEITASAGNSQPSNTQTITFVADKATATVSGIE
                     VIGNYALADGNAKQTYKVTVTDANNNLLKDSEVTLTASPANLVLTPNGTAKTNEQGQA
                     IFTATTTVAAKYTLTAKVSQADGQESTKTAESKFVADDKNAVLTASSDVTSLVADGIS
                     TAKLEVTLMSANNPVGGNMWVDIKTPEGVTEKDYQFLPSKNDHFVSGKITRTFSTSKP
                     GVYTFTFNALTYGGYEMKPVTVTITAVDADTAKGEEAMN"
     gene            complement(6184..7041)
                     /locus_tag="ACHOUK_003827"
     CDS             complement(6184..7041)
                     /locus_tag="ACHOUK_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9899179.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIAISLHFDSQQSFSREFKKLFGCSPRV
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMKNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNEDFVEKDSNDVVC
                     EIFIPVRPV"
     gene            7290..8159
                     /locus_tag="ACHOUK_003828"
     CDS             7290..8159
                     /locus_tag="ACHOUK_003828"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFP9899180.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTREELFITSKLWVQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            complement(8319..8912)
                     /gene="rclC"
                     /locus_tag="ACHOUK_003829"
     CDS             complement(8319..8912)
                     /gene="rclC"
                     /locus_tag="ACHOUK_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFP9899181.1"
                     /translation="MEKYLHLLSRGDKIGLALIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQGSNESSSTLKTEY"
     gene            complement(8924..9160)
                     /gene="rclB"
                     /locus_tag="ACHOUK_003830"
     CDS             complement(8924..9160)
                     /gene="rclB"
                     /locus_tag="ACHOUK_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFP9899182.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     DAIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            complement(9269..10594)
                     /gene="rclA"
                     /locus_tag="ACHOUK_003831"
     CDS             complement(9269..10594)
                     /gene="rclA"
                     /locus_tag="ACHOUK_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFP9899183.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVINGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREERDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKRLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGKRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            10820..11674
                     /gene="rclR"
                     /locus_tag="ACHOUK_003832"
     CDS             10820..11674
                     /gene="rclR"
                     /locus_tag="ACHOUK_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFP9899184.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWSVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
BASE COUNT         3193 a         2608 c         2882 g         3067 t
ORIGIN      
        1 atgaccgcta cggttaacgg tggttatttt gggaggcgtt aacacgtagc ggcttgtagt
       61 tgtaagtctt gaacaggtac aaagcctgaa atcaggagga tatcgctatc aaacttcctt
      121 tatttcttct cgcagtttcg catcttatag aagaatcctg tatttccatc ttccacgatg
      181 aagcgatcgt taaaagttgg acgacgaata aatgtcactc cacctattgt tgctttttgt
      241 tttataccat catcaacatt ttccagatct ggagagtaca attgatctcc atttggcatt
      301 tgaacgatga aattgtttcc attatcgaga actgttgcgg attttgacag aatttgagta
      361 gcggtatcca ggctggaaat cctaatgtca caaacatatt tgcctggccc cagatcttct
      421 tctgcaaata caggtaagga aaataatgca aatgcgagga tgagattctt attcatgcaa
      481 actccaggaa gatcaataaa agcaatatgt tacataaaaa gcacgtaaat gaaaagagcg
      541 attggttaat gagtaatgct attaatcccc gcatggccta cttttcattg gctgcggtgt
      601 tgaacctcat tgaatatggg caagactctg actgagtgac cgacgtaaat aggtagatgc
      661 ataatcgctt accgccctgt aactgcaaac ggtgcccagg cttctggctg aataccacgg
      721 ctttgcatcc acggaacggc gtgtagctgc tgattgaaag ggttaacttc aacctggttt
      781 accgctggaa cgacgttatt aaaggcgata aggtcagcca gtcggtcagg atggaagtta
      841 ctgacgccaa tagcgcgaat tttaccggcc tgttgcagct cttccatggc acgccaggca
      901 ccgtggacat caccgtaagg ctgatgaatc aaatacaggt caacgtaatc aagttgcagt
      961 cgattcagag agcgttcgaa ctgggctttg gccccttcgt aactggtatc ctgcaaccag
     1021 agtttcgtgg tgacaaagag ttcgttacgg gcgataccgc tttgtttcag tgcgttcccg
     1081 acctggggtt cattttgata ggaggcggcg gtgtcaatca gtcgataacc ggtgttaata
     1141 gcgtcaatga ccgctctttc gcattcggcg gcatcggtca tctgaaatac accaaagccc
     1201 agcaggggca tttcaatacc gttattcagt tttacttttt gcatggtgtt atccttccgt
     1261 tatcgttcat gagaagcata acgtaaagag aagagctcga ttagacggca ggatttgtca
     1321 ggggttatga acgaaattca taaatctgtt tgagtgttgc gatgggtagt gcaagttcga
     1381 tatctccgca atttacagtc cgatgaagga aaatgaatat ccataaaaaa tatatcggtt
     1441 tatcctggcg tatataccta cttcgacgta tttccaatag ttgctaatta aaggcaggtc
     1501 attattattc actctgaata atgaattatt cactgtccgc agagtaagaa atataactta
     1561 agtatctatt taatgacttg cacaaaaagc taaattttca cccataaata aaaatataat
     1621 cccgcgccca accacctgat gagtggctac aggcactgga tatattaggt ggcggtgtac
     1681 tttcttacat aaaggtattt ccttttctgc ggaaaaggaa accgggaaat ccccggtttt
     1741 tctgacaagc agacgccatt atttgtgtct gcctatgttc gttaattcgt tcatcaggaa
     1801 attatctcaa tgtcacgtta taaaacaggt cataaacaac cacgatttcg ttattcagtt
     1861 ctggcccgct gcgtggcgtg ggcaaatatc tctgttcagg ttctttttcc actcgctgtc
     1921 acctttaccc cagtaatggc ggcacgtgcg cagcatgcgg ttcagccacg gttgagcatg
     1981 ggaaatacta cggtaactgc tgataataac gtggagaaaa atgtcgcgtc gtttgccgca
     2041 aatgccggga catttttaag cagtcagcca gatagcgatg cgacacgtaa ttttattacc
     2101 ggaatggcca ccgctaaagc taaccaggaa atacaggagt ggctcgggaa atatggtaca
     2161 gcgcgcgtca aactgaatgt cgataaagat ttctcgctga aggattcttc gctggaaatg
     2221 ctttatccga tttatgatac gccgacaaat atgttgttca ctcagggggc aatacatcgt
     2281 acagacgatc gtactcagtc aaatattggt tttggctggc gtcatttttc aggaaatgac
     2341 tggatggcgg gggtgaacac ctttatcgac catgatttat cccgtagtca tacccgcatt
     2401 ggtgttggtg cggaatactg gcgcgattat ctgaaactga gcgccaatgg ttatattcgg
     2461 gcttctggct ggaaaaaatc gccggatatt gaggattatc aggaacgccc ggcgaatggt
     2521 tgggatatcc gcgcagaggg ctatttacct gcctggccgc agcttggcgc aagcctgatg
     2581 tatgaacagt attatggcga tgaagtcggg ctgtttggta aagataagcg ccagaaagac
     2641 ccgcatgcta tttctgccga ggtgacctat acgccagtgc ctcttctgac actgagcgcc
     2701 gggcataagc agggcaagag cggtgagaat gacactcgct ttggcctgga agttaactac
     2761 cgaattggcg aacctttggc gaaacaactc gatacggata gcattcgcga gcgtcgggta
     2821 ctggcaggca gccgctatga cctggttgag cgtaataaca acatcgttct tgagtaccgc
     2881 aaatctgaag tgatccgtat tgctctgcct gagcgtattg aaggtaaggg cggtcagaca
     2941 ctttccctgg ggcttgtggt cagcaaagca actcacggac tgaaaaatgt gcagtgggaa
     3001 gcgccgtcat tactggctga aggtggcaaa attaccggtc agggtagtca gtggcaagta
     3061 acgctcccgg cttatcgtcc aggcaaagac aattattatg cgatttcagc agttgcctac
     3121 gataacaaag gcaatacctc aaaacgcgtg cagacagagg tggtcattac cggagctggt
     3181 atgagcgccg atcgcacggc gttaacgctt gacggtcaga gccgtattca aatgcttgct
     3241 aacggtaatg agcaaaaacc gctggtgctg tctctgcgcg acgccgaggg ccagccagtc
     3301 acgggcatga aagatcagat caagactgaa ctaactttca aaccggctgg aaatattgtg
     3361 actcgttccc tgaaggccac taaatcacag gcaaagccaa cactgggtga gttcaccgaa
     3421 actgaagcag gggtgtatca gtctgtcttt actaccggaa cgcagtcagg tgaggcaacg
     3481 attactgtta gcgttgatgg catgagcaaa accgtcactg cagaactgcg ggccacgatg
     3541 atggatgtgg caaactccac cctgagcgct aacgagccgt caggtgacgt ggttgctgat
     3601 ggtcagcaag cctatacgtt gacgttgact gcggtggact ccgagggtaa tccggtgacg
     3661 ggagaagcca gccgcttgcg atttgttccg caagacacta atggtgtaac cgttggtgcc
     3721 atttcggaaa taaaaccagg cgtttacagc gccgcggttt cttcgacccg tgccggaaac
     3781 gttgttgtgc gtgctttcag cgagcagtat cagctgggca cattacaaca aacgctgaag
     3841 tttgttgccg ggccgcttga tgcagcacat tcgtccatca ccctgaatcc tgataaaccg
     3901 gtggttgggg ggacagttac ggcaatctgg acggtaaaag atgcctatga caaccctgtg
     3961 accagcctca cgccggaagc gccgtcatta gcgggtgccg ctgctgaagg ttctacggca
     4021 tcgggctgga caaataatgg tgatgggacg tggactgcgc agattactct cggctctacg
     4081 gcgggtgaat tagaagttat gccgaagcta aatggacaga atgcggcagc aaatgcggca
     4141 aaagtaaccg tggtggctga tgcgttatct tcaaaccagt cgaaagtctc tgtcgcagaa
     4201 gatcacgtaa aagccggcga aagcacaacc gtgacgctgg tggcgaaaga tgcgcatggc
     4261 aacgctatca gtggtcttgc gttgtcggca agtttgacgg ggaccgcctc tgaaggggcg
     4321 accgtttcca gttggaccga aaaaggtaac ggttcctatg ttgctacgtt gactacaggt
     4381 ggaaagacgg gcgagcttcg cgtcatgcct ctcttcaacg gccagccagc agccaccgaa
     4441 gccgcgcagt tgacggtcat cgccggagag atgtcatcag cgaactctac gcttgttgcg
     4501 gacaataagg ctccgaccgt caaaacgacg acggaactca ccttcaccgt gaaggatgcg
     4561 tacgggaacc cggtcaccgg gctgaagcca gatgcaccag tgtttagcgg tgccgccagc
     4621 acggggagtg agcgtccttc agcaggaaac tggacagaga aaggtaatgg ggtctacgtg
     4681 tcgaccttaa cgctgggatc tgccgcgggt cagttgtctg tgatgccgcg agtgaacggc
     4741 caaaatgccg ttgctcagcc actggtgctg aacgttgcag gtgacgcatc taaggctgag
     4801 attcgtgata tgacagtgaa ggttaataac caactggcta atggacagtc tgctaaccag
     4861 ataaccctga ccgttgtgga cacctatggt aacccgttgc aggggcagga agttacgctg
     4921 actttaccgc agggtgtgac cagcaagacg gggaatacag taacaactaa tgcggcaggt
     4981 aaagcggaca ttgagcttat gtcaacggtt gcgggagaac acaatatttc cgcttcggtg
     5041 aatggtgctc agaagacggt cacggtgaaa ttcaacgcgg atgccagcac cggtcaggca
     5101 aacctgcagg tagacgccgc tgctcaaaaa gtggcaaacg gcaaagatgc ctttacgctg
     5161 acggcgaacg ttgaggataa aaatggtaac cctgttccag ggagcctggt gacctttaat
     5221 ctgccccggg gtgtcaagcc gcttacaggc gataatgtct gggtgaaagc caacgatgag
     5281 gggaaagcag agttgcaggt ggtttcagtg actgccggaa cgtatgagat cacggcatcg
     5341 gcagggaata gccagccttc gaatacgcag actataacgt ttgtagccga taaggctacc
     5401 gcaaccgtct ccggtattga ggtgattggc aactatgcac tggcggacgg caatgccaaa
     5461 cagacgtata aagttacggt gactgatgcc aataacaacc tgttgaaaga tagcgaagtg
     5521 acgctgactg ccagcccggc aaatttagtt ctgactccca atgggacggc gaaaactaat
     5581 gagcaaggac aggctatttt caccgccacg accactgtcg cagcgaaata tacactcacg
     5641 gcgaaagtga gtcaggccga cggtcaggaa tcgacgaaaa ctgccgaatc taaattcgtc
     5701 gcggatgata aaaatgcagt actcaccgca tcatctgatg tgacttctct ggtggcggat
     5761 gggatatcga ctgcgaagct ggaggtgaca ctgatgtcgg caaataaccc cgttgggggg
     5821 aatatgtggg tcgacattaa gacgccagaa ggggtgacgg agaaggatta tcagttcctg
     5881 ccgtcgaaaa atgaccattt cgtgagcgga aaaatcacgc gtacatttag taccagcaag
     5941 cctggtgtct atacgttcac atttaacgcc ctgacgtatg gcgggtacga aatgaagcca
     6001 gtgacggtga ccattaccgc ggtggatgcc gatacggcaa agggcgagga ggcgatgaac
     6061 taatattaag aatatggaag taacattcta ataaacaata atggcgttga catcttcaac
     6121 gccattattt attgaattgc ctgacagtag tctcgttata agttcattta gatatagcga
     6181 cagctaaacc ggacgaacag ggataaatat ttcgcaaaca acatcattgc tatccttttc
     6241 tacgaaatct tcattgtaat aaaaccgctc aatatcactg ccgttcactc tggctaaccc
     6301 cctggcgggt aattccatca gatagaggtt tcgcgcccat tcagggtagt gctcccactc
     6361 accttcatag cggaaagagg catacagtcc tcggttaatt gtccactctt tcggcatctc
     6421 tgaatcggtt tgtttgcata ccgtaccggc aaaagtttca actgtgagta aatcgaccga
     6481 ttttgttgac ggttcaaacg tggagacgaa atagatatcc gtcttaaaat tcttcataca
     6541 tctggctaaa tgatgacgtc gcagtttgac ttcttcatcc ggtgatttat tcgacacttc
     6601 aatatcatat ttaaatgaat tgccaaaaat aggtgtctca ggaaagttga ctaatctaca
     6661 ctccgtcttt tgctgttgac gtattaaaaa agaagggaag atatttgcga gatcccaata
     6721 atcacggtgg cggtatacac ggggagagca gccgaataac tttttaaatt cgcggctgaa
     6781 tgactgctgt gaatcaaaat gcaaagagat tgcaatatca agcatagatt tcgcggtgag
     6841 ccggacaaga atggcggcac gacaaagcct tcgtttgcga atgtattctc ccaaaggcac
     6901 atgcatgaaa tttcggaaca gaagctggat gttgcgtctg ctgtagccag atttctgtgc
     6961 gatatcttcg attgaaatag ggtgctcaag attgcactca atccactcca ggagctgctg
     7021 tagaatcttc tgcctgatca tcacattctc cgttaattct ttgtcatatc gcacattttc
     7081 gttatgccct gggccctaag tgtatcaaaa acagcaaggg cgaaggcaaa agcactggta
     7141 gcgtctgctt cgttgtcccc gaaaatagag aggtttcagt cctacattat taatgaattt
     7201 tttgcataag tgatatcaaa atccacatac taatttgagg ttacgtttta acgtagactc
     7261 attgttcatg cctaatggag ggactgacag tggaattttc cgtattaagt aacaatctga
     7321 aaatgccgat gatgggattt ggtgtttttc aggttaccga taaaaacgtg tgcaaacagt
     7381 cagtgttgaa cgctatccgc acggggtatc gactcatcga cactgcagcg gtatacggta
     7441 atgaagatgc cgtcggtgaa gccgttcgtg aagctatatc cgaaggttta tgtacccgag
     7501 aagagttatt tataacgtct aaattgtggg tgcaggatat gttgaatcag gatacggcag
     7561 cagcaggtat tgaagcatca ttaaaaaaat cgggactaga atacttcgac ctctatttat
     7621 tgcatcaggc tatgcgcgat tatttcagtg cgtggcgtgc actcgaagat gcctatgaag
     7681 agggcaaatt aaaagcgatt ggggtttcca atttctatcc tcatgttctg gcgaactttt
     7741 gtgaaacggt aagagttaaa ccgatggtca accaggtcga gttgcatccc tattttgccc
     7801 aaccagaggc gctggcaacc atgaagtatt ataacgtgca gcctgaagca tgggctccgt
     7861 taggtggtgg acgacataaa ccctttgaaa ataatctgct tcagagtatt gcagatgccc
     7921 atcaaaaatc gatttctcaa gtcattctgc gttggaatat tcaacgggga gtggtcgtta
     7981 ttccgaaatc gacacatcaa cagcgtatcg aagaaaattt tgctatctgg gatttctcac
     8041 tgacagagaa agaaatggca caaattagtt cgcttgattt gggttatgtt ggggagtcgg
     8101 taaaacattt taatcctgaa tttgttcgtg gttgtcttgc tgtaaaaata catgattgat
     8161 attaatcatg tattttacct gagacgacaa gaatctttta acaggggagt gatattgatc
     8221 ttcactctgt catatctccg gtaatatggc gtcaggcttc atcatcagaa agggggcctg
     8281 acgcctgaaa aagtgaacaa cagacagtgt tcggattatc aatattcagt ttttaacgtt
     8341 gaactggatt cattactgcc ttgtttaaga atttcccgcg ccgaatctgc cattatcatg
     8401 acagcacctg ccagcatcag agtatctttc aataccagac gaccagcacc ggataaataa
     8461 gggaaaccat gatgagcgtc acccaatgcg ggtacccatg cctccggggt ggtgattaaa
     8521 aatgagagtg ttaccagcgg tgtggtaaat gccatcagcc ctcccaataa acctaaccag
     8581 cgattgacag gattagccaa aaccagcaga gcaataatca cctccacgac gccaagaccg
     8641 ttggaaaaac cataggtatt attggccgat tgccatgccc ttgcttctgg tttgtattcg
     8701 ccttcgtgag tcagatactg tttatagtct tccgggtgtt cataaaagaa cgacattagt
     8761 ggactgtttg cgacgaatgg tgtaatgctg tctgcctcgt aagggacaaa ctttaataac
     8821 ccaatccaca taaaaacaat cgcaatactc agacgaatca atgccaggcc aattttatct
     8881 ccccgactta acaggtgcag gtatttttcc attgtgaata tccttattta tacaatactg
     8941 ctgttgcggt tgaattatta tcaattcgca tcgatgtgac tttccaggat gaagcattat
     9001 attttctggc tatatcagca atggcatcct ccagaactgt cggtgatgct gatactgacg
     9061 agacgctgat atgtttcaga attattttat catctgcatt ggtggaaaat gccataacgc
     9121 cagataatag tgttgcaaat aaaacggatt ttttaaacat ataaaatacc atcaatgaag
     9181 tgattaagaa gttatagagt aacagggaat taataagatt cttttctctg agacgccaga
     9241 atatttgttc tggcgtctga ttttgagttt atttgactaa tgaaaataga tcattgagtg
     9301 attcgctcat cgacggatga gtaaatatct gatcgcgtaa tatgctataa ggcagcccgg
     9361 catccatcac cattttcact atattaatca tctcgtggga gtcaacacac agcagtgatg
     9421 cccctaatat acgttgggtt ttattatcaa caatcgcttt taatacccca cgggtatcgt
     9481 tcatcactct ggcgcgcgga attgcagcta caggcaatgt caccacctga atatcagcac
     9541 cactctctct ggcttgttct tctgtcatac caaccctgga caggggcggt gtcataaata
     9601 cggaataagg cacatttttc cgatcatcag tactacgttt gccttcaccc agtaactcat
     9661 cacgtacaat gcggtaatca tccagtgata tgtaagtaaa ttgcagcccg ccggtaacat
     9721 ctcccatcgc ccaaatattg tctgcggtgg tatgtaatcg cttgtcaacg acaattgccc
     9781 cgcgctcgtt tactgcgata ccggcatttt ctggatgtaa cgaagcggta gccggttgac
     9841 gaccggaagc tattaacagt gcatccaccg ccagttgggc gtgctcgcta tgcacttgca
     9901 cttgattttc atggtgactg attcgctcca catgggcatt gaggataata tcgacgccct
     9961 gatcgcgtaa aatcgtcgcg atattatcag caatatcccg ttcttcccga ggcaaaaaca
    10021 gcgaagctgc ttctaaaatg gttaccttgc tgccaaaatt agcgaacata gaggcgaact
    10081 caacgccaat atatccgccg cccaaaatac ctaaatgccc aggcaattct tttagattaa
    10141 gtaatccggt gctgtcatat actcctggcg tggtggtaat tccaggaatt ggcggaacca
    10201 cggtttgtgc accggtatta ataaaaattt tctcgccatg aatttccaga tttccctcag
    10261 gccgatgaac acgcaggcta tgattattga taaactccgc ctggccgttg atcacgtcga
    10321 tattgggcat atctgcaaga ttatgaaaat tcttattacg taaaaaatta accacttcat
    10381 ttttacgctg tatggctcgg acaaaatctg tgtgctgctg tgcgtcatga accaatgttt
    10441 tggttgggat gcagccgata ttaatacagg tcccgccata cattgcattt gattgttcga
    10501 tgagagccac tcgccaacct gcttttgcca gcgtgacggc taatgttttt ccagccttgc
    10561 caaaaccaat aatcactgcc tgatatttat tcatgatgat ttcctgtcga aacgcgttca
    10621 tttgacatca tgactataga cgcaaaagag caaataacat ttcttcacaa cgcttgatat
    10681 tttgtcttat cgtctggtat tttctttttc ttaatcagaa tcacaaaaca aaaatcatga
    10741 ttaactattt gatggtatta gattagttgt ttactaagat tgttggtgtt tgtaatcaaa
    10801 aaccactcag gagtctgata tggatgccct tagccgtttg ttgatgctta acgctccaca
    10861 aggaacgatc gataagaatt gcgtgttagg aagtgactgg cagcttccac atggtgccgg
    10921 ggaattatca gttattcgtt ggcatgcgtt aacgcaagga gcggcgaagc tggaaatgcc
    10981 gacgggagag atttttacat tacgcccggg aaatgtggtc ctgctaccac aaaattcagc
    11041 tcatcgcctg agtcatgtcg ataatgaatc gacctgtatt gtctgtggta ctcttcggct
    11101 gcaacattcg gcacgttatt ttttaacgtc tttgccggaa acgctgtttt tagcaccggt
    11161 taaccacagc gtggaatata actggctgag ggaggcgatc ccgtttttac aacaggaatc
    11221 cagatcggca atgccgggag tggatgcact gtgtagccag atctgcgcta cattctttac
    11281 cctcgcggtg cgtgagtgga ttgcacaggt taatactgag aaaaacattc tcagtttgct
    11341 tctgcatcca cgtcttggtg cggtaataca gcaaatgctg gaaatgccag gacacgcctg
    11401 gagcgtcgaa tcgctggcca gcatcgccca catgtcccgg gcaagttttg cccagctttt
    11461 ccgtgatgtt tccggaacca cgccgctggc tgtattaaca aagttgcgtc tacaaatagc
    11521 ggcccagatg ttttcccggg aaatgctccc tgttgtggtg atcgctgagt cagtaggcta
    11581 tgccagtgaa tcatcttttc acaaggcgtt tgtccgcgag tttggttgta ctccgggaga
    11641 atatcgggaa agggtcagac agcttgcacc ctgaataaaa ccgccagaaa tcagggcaaa
    11701 gataatccgc attccgggag ttgtgagcct ttcaactatt tctatttcca
//
LOCUS       DAZKIP010000125        11722 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZKIP010000125 DAZKIP010000000
VERSION     DAZKIP010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.126"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..667)
                     /locus_tag="ACHOUK_003833"
     CDS             complement(<1..667)
                     /locus_tag="ACHOUK_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899185.1"
                     /translation="MYNNLYALTESLFWVDRLLEQEYENIGLNELLVELAEYNKRIDS
                     FIIEQTNSVQESRLNVYMAGNRNIERYKSGLFSMDRVILDDVLYSLSLRYRVNLSENV
                     VEKFIESDSKEKVEHLRKEIARFIRFAKENFDLIRGEFIVFVRRESSIKDSERANGIL
                     EDNSDTNFLFRYLPPNIARLYESRLSVQNIERLGNTNKIRLLNRNVLTNEIMLKIKGC
                     ESSY"
     gene            1680..1856
                     /locus_tag="ACHOUK_003834"
     CDS             1680..1856
                     /locus_tag="ACHOUK_003834"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899186.1"
                     /translation="MNMNEDEIYRHIRQALSSAPRNQYTVELHLQMIKYADELEHITA
                     KAFCEGTGLNTDLL"
     gene            1896..4334
                     /locus_tag="ACHOUK_003835"
     CDS             1896..4334
                     /locus_tag="ACHOUK_003835"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFP9899187.1"
                     /translation="MAIKKTELYSSLWASCDELRGGMDASQYKDYVLTLLFMKYVSDK
                     YKGDPYGMIVIPKGASFDDMVALKNDKEIGDKINKIIHKLAEENGLKGVIDEADFNDE
                     DKLGKGKEMIDRLSKLVGIFEGLDLSSNRVEGDDLLGDAYEYLMRHFATESGKSKGQF
                     YTPAEVSRILAKVIGITPDTPRDATVYDPTCGSGSLLLKVNDEARRGLSIFGQEMDNA
                     TSALARMNMILHNNATAKIWQGNTLSDPQWKEANGKLKAFDFAVANPPFSNKNWTNGL
                     TPKKDPFERFGWGIPPEKNGDYAFLLHIIKSLKSTGKGAVILPHGVLFRGNAEANIRE
                     NLIKQGYIKGVIGLPANLFYGTGIPACIIVIDKEHAHSRKGIFMIDASRGFIKDGNKN
                     RLRSQDIHRIVDVFNHQRTVPGYSRMVPSSEIAGNDYNLNIPRYIESGEPEDLHDLTA
                     HLQGGIPVRDVDALKDYWRVFPALRNVLFADDHPGYCRAQVDAQQVKPTILAHQEFKD
                     FATRSLLPFKSWVKEAGLEEIRKGDKPKALIHDISEMLLAQYANSELLNKYSVYQILM
                     DYWADVMQDDVYVIMQDGWKAAAQIRELQPVKGKDGKNIWKETHDFEFTKRRYKADVL
                     PRSLVEIRCFPELLDALNRAQERSEEASRQLVEFIEEQAGEEDLLAEVKNDKDKVTQK
                     LVNARLAQLKPPPADPDELAVLNRCLALIKAEASAKKALKAAQEALDRAVFKHYPTLD
                     EAAIKTLVVQDKWLATLQAGIKAEIERITQQLASRVKELEERYAEPLPALEAAVEALS
                     EKVAGHLRAMGLEW"
     gene            4334..5683
                     /locus_tag="ACHOUK_003836"
     CDS             4334..5683
                     /locus_tag="ACHOUK_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFP9899188.1"
                     /translation="MVDLMVNEQRSGTAGKDVPAGYKLTEVGVIPEDWVVISIDDCTT
                     KVGSGKTPTGGASVYIDSGRPFVRSQNVDWGRLDLADVVHITDETHNTFSGSEIQQDD
                     VLLNITGASIGRCAKADFRVIGGNVNQHVCIIRTEAKKLSPSLLVELINSNYGQNQID
                     SYQAGGNREGLNFSQVKNLKFAITPNSFEQTEIVRTLNDINAFTLALENLIAKKQAIK
                     AAAMQQLLTGKTRLPQFALRKDGSAKGYKKSELGEIPEDWNCINLGELGNCIIGLTYK
                     PEDTSDYGTLVLRSSNIQNNHLAYDDNVFVSIEPPERTIVKEGDILVCVRNGSRQLIG
                     KCALIDKNAQGSAFGAFMSVFRTDIYGFTFHQFQSDIIQKQIAEVMGATINQITNKDM
                     LGFKIPLPENTEEQAAIAAILSDMDKEIQILQQRLDKTRQLKQGMMQELLTGKIRLI"
     gene            5696..6352
                     /gene="darT"
                     /locus_tag="ACHOUK_003837"
     CDS             5696..6352
                     /gene="darT"
                     /locus_tag="ACHOUK_003837"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system toxin DNA ADP-ribosyl
                     transferase DarT"
                     /protein_id="HFP9899189.1"
                     /translation="MAYDYSASLNPQKALIWRIVHRDNIPWILDNGLHCGNSLVQAEN
                     WINIGNPELIGKRAGHPVPVGTGGTLHDYVPFYFTPFSPMLMNIHSGRGGIKQRPNEE
                     IVILVSNLRNVAAHDVPFVFTDSHAYYNWTNYYTSLDSLDQIDWPILQARDFRRDPDD
                     PAKFERYQAEALIWQHCPISLLDGIICYSEEVRLQLEQWLFQRNLTMSVHTRAGWYFS
                     "
     gene            6349..7416
                     /gene="darG"
                     /locus_tag="ACHOUK_003838"
     CDS             6349..7416
                     /gene="darG"
                     /locus_tag="ACHOUK_003838"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin DNA ADP-ribosyl
                     glycohydrolase DarG"
                     /protein_id="HFP9899190.1"
                     /translation="MITYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPENMK
                     VYALACKQKQVITGKMFITETGELMGPRWIVNFPTKQHWRADSRMEWIEDGLQDLRRF
                     LIEENVQSIAIPPLGAGNGGLNWPDVRAQIESALGDLQDVDILIYQPTEKYQNVAKST
                     GVKKLTPARAAIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQDDILKLRFEAH
                     YYGPYAPNLNHLLNALDGTYLKAEKRIPDSQPLDVIWFNDQKKEHVNAYLNNEAREWL
                     PALEQVSQLIDGFESPFGLELLATVDWLLSRGECQPTLDSVKEGLHQWPAGERWASRK
                     LRLFDNNNLQFAINRVMEFHC"
     gene            7435..10530
                     /locus_tag="ACHOUK_003839"
     CDS             7435..10530
                     /locus_tag="ACHOUK_003839"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015872650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFP9899191.1"
                     /translation="MSTVGQRERATQQRLIQFFVEDLGYRHLSHRTGYTGNSNIDTNI
                     LSAWLKRRGVNDTLITRALRQLDSAAALGEGKKLYYANKEVYRLLRYGVKDKEGAGEQ
                     NQTVWLIDWANPQANDFAIADEVTVKGEYCKRPDLVLYVNGIALGVIELKRSSVHISE
                     GIRQNLDNQKKTFIRDFFTTMQLVMAGNDTQGLRYGTIETPEKYYLEWKEDNPGDYTH
                     KLDFHLSRLCSKSRLLDIVHNYIAFDVGVKKLCRHNQFFGVEAAKQHIARREGGIIWH
                     TQGSGKSLTMVWLAKWIRENVPNSRVLIVTDRTELDEQIESVFMGVNEDIYRTSSGND
                     LIATLNHPNPWLICSLVHKFGRRSEAEDNAATDAFITELQQSLTKTFRAKGDLFVFVD
                     ECHRTQSGKLHNAMTAILPDALFIGFTGTPLMKKDKKKSVEVFGPYIHTYKFDEAVAD
                     GVVLDLRYEARDIDQYLTSEKKVDDWFEAKTRGLSNLARTQLKQKWGSMQKLLSSKSR
                     LEQIVNDILLDMDTRPRLMDGRGNAMLVCSSIYQACKVYEMFSQTELAGKVAIVTSYR
                     PDAASIKGEETGEGLTEKLSKHATYRKMLADYFEQSEEKAAARITEFEREVKQRFIEE
                     PGQMRLLIVVDKLLTGFDAPSATYLYIDKTMTDHTLFQAICRVNRLDGEDKEYGYIID
                     YKDLFRSLDKAITDYTTGAFDDYDRDDVAGLLKDRLEQAQLDLDSALEMVRSLCEPVK
                     TPRSLQDYQHYFCGESGENQTSLNEKEALRLTFYQCVARLLRAYASIANEMPEAGYTA
                     EEVESIRAEVAEFEKLRYEIKLTSGDLLDMKRFEPAMRHLLDMYVRADDSEVLMDFEE
                     LSLIELIVEKGLAATVALPDDIRNNQDAMAETIENNVRKTIVDENPVNPKYYGRMSQL
                     LDELITLRRQNALNYQQYLERIRDLCKQVIRPEQTAGACYPATMDTQAKRAFYDNFGH
                     DEVLATRIDTTIRYTKRAEWIGDRFKEREIANALREETASYNIDIDEVIALARQQKEY
                     H"
     gene            10530..11246
                     /locus_tag="ACHOUK_003840"
     CDS             10530..11246
                     /locus_tag="ACHOUK_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012773552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFP9899192.1"
                     /translation="MPIMQIGSLHLLVNRKAIKNLHISVLPPDGKVRVSAPEHMTETA
                     IRMAVASRFRWIRKQQQDFARQPRQSEREMVSGECHYLWGRRYRLLVIERHGRHEVKV
                     AGNNKLQLYVQPGTRDENKALALSEFYRHELKQQVAKLLPEWQDRIGVPLTFWGIKKM
                     KTFWGTCNTTTKRIWLNLELIKKPPECLEYILVHELVHLLERHHNARFRLHMDRFIPD
                     WRERRDLLNNMPLAFEAWEY"
     gene            complement(11468..11593)
                     /locus_tag="ACHOUK_003841"
     CDS             complement(11468..11593)
                     /locus_tag="ACHOUK_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001452123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899193.1"
                     /translation="MHTNDRSLTMVDLFCCAGATARLVSEAGSEELRITVKEIGE"
BASE COUNT         3243 a         2728 c         2980 g         2771 t
ORIGIN      
        1 tatacgagct ttcgcagcct ttaattttca acataatttc attcgttaaa acatttctgt
       61 ttaacaaccg aattttattt gtgttgccca gacgttctat attctgtacg gatagtctac
      121 tttcatacag acgggcaata ttagggggga gatatcgaaa taggaaatta gtatcagagt
      181 tatcttctaa aattccatta gctctctccg aatctttaat agaggattct cttcgtacaa
      241 aaacgataaa ctctccacgg ataagatcaa agttttcttt agcaaacctg ataaacctag
      301 caatttcctt cctgagatgt tcaaccttct ctttgctatc gctttcaata aatttttcaa
      361 ctacgttttc tgatagattt accctgtatc gtaggctcaa tgaatataga acgtcatcaa
      421 gaatgactct gtccattgaa aacaaaccac tcttatacct ttcaatatta cgattccccg
      481 ccatgtaaac attcagccgg gactcctgta cagaatttgt ttgttcgata atgaatgaat
      541 ctatcctttt attatattca gctaattcaa ctagcaattc gttcaatccg atattttcat
      601 attcctgttc taaaagtcga tcaacccaaa aaaggctttc tgtgagcgca tagaggttgt
      661 tgtacatact agctcctttt taccaagctt cagtactgtg ttctcaagat tactcagtgg
      721 ttttcacaca gagtgcactc tcattttaca tttaaatgta cttcctagca tcctgctgtt
      781 agtgccggat tacagcactt atcaactgta tatttagaaa acaaactgcg cataatccta
      841 gtgagttgtt atgtccgctc ctcgctcaaa gcagaccatt cgctcagtta acgcatcagt
      901 gtcgtgctaa aagtagacgt ggttaacaac aatgtgagtt aatcaacgga gagcaggtca
      961 actctaacct gtatcaaaaa aacaaatgtg tcacaaagat gccgcatgcc ttaatttgag
     1021 tataattgag acggaagtag acggtttatg gctaaatatt gttgatatat agctatttga
     1081 agagttgtag cgcaagtgta tagcccaaac taataacgct accccttacc tttgacgcct
     1141 cttatcaaag agctgcgatg atgcacagcc cgcacgaggc aatattcgca caagaatcag
     1201 gttcagaaat aacctgctct ggcatggtgg ggagagtatc actgatcagg ctttcgttaa
     1261 gatgcggcgg gatgaggccg tgttgcgtga gttgcagcag tacaagatcg cccgcagagg
     1321 gtaagctaca gcgcgagatc ctaggtttct atcagggatt cggcaaagcg ggcgcagttg
     1381 tcgaccaagg cggtgaaatc tatagtggca ttataaggga acgaacaata gatcgagtaa
     1441 atcgcagtca gggacaagga ttgtagtcta tagagaagtc tcctgtaacg gttttcagga
     1501 ggctaattcc gacgcctgat tttgcaggcg cggagggaga ggatactgcg accattaatc
     1561 cgccacaaag catttacgtg cataaatcac gcagtcagat ccgactctca ctgtagttct
     1621 tgaacctgat accacattta gctaatttag ctaacttatc cagtatacca gtggttgcca
     1681 tgaatatgaa tgaagacgag atttatcgtc atatccggca ggcactttcc agtgcgccgc
     1741 gtaaccagta caccgtcgag ctgcacctgc aaatgattaa atatgccgac gaactggaac
     1801 acattacggc aaaagccttt tgcgaaggca ccggtctgaa taccgatctg ctgtaaccga
     1861 cttacggcac attatcgcgc aaaacgagat atttaatggc tatcaaaaaa accgaactct
     1921 attcttccct gtgggcaagc tgcgacgaac tgcgtggcgg gatggacgcc agccagtaca
     1981 aagactatgt actgaccctg ctgtttatga agtacgtttc cgataagtat aaaggcgatc
     2041 cttacgggat gatcgtgata cccaaaggag ccagctttga cgatatggtt gcgctgaaaa
     2101 atgacaaaga gatcggcgat aaaatcaaca aaatcatcca caagctggcg gaagagaacg
     2161 gcctgaaggg cgtcatcgac gaagccgact ttaacgacga agataaactc ggcaaaggca
     2221 aggaaatgat tgaccgcctg agcaagcttg tcggtatctt tgaagggtta gatctctcca
     2281 gcaaccgggt cgaaggcgac gatctgctgg gcgatgccta tgaatatctg atgcgtcact
     2341 tcgcaaccga atccggcaag agtaaaggac agttttacac tccggcggag gtgtcacgta
     2401 ttctggcaaa ggtgattggt attactcccg atacgccgcg agatgcaacg gtttatgacc
     2461 caacctgtgg ctctggttcg ctgctgttga aagtgaatga tgaagcccgg cgcggactgt
     2521 cgattttcgg ccaagagatg gataacgcta ccagtgcgct ggcgcggatg aacatgattc
     2581 tgcataacaa tgccaccgcg aaaatctggc agggcaatac gctgagtgat ccgcagtgga
     2641 aagaggcgaa cggcaaactg aaagccttcg attttgccgt ggcgaacccg cctttttcca
     2701 ataaaaactg gaccaacggg ctgacgccga aaaaagatcc gtttgaacgc ttcggctggg
     2761 gtattccgcc ggaaaaaaac ggcgattatg cttttttgtt gcatatcatt aaaagcctga
     2821 aaagtaccgg caaaggcgcg gtgatcctgc cccacggcgt actgtttcgc ggtaacgccg
     2881 aagcgaatat ccgtgaaaac ctaatcaagc agggctatat caaaggcgtt attggtttgc
     2941 cagccaacct gttttacggc accggcatcc cggcgtgcat cattgtgatt gataaagagc
     3001 acgcccacag ccgcaaaggc atttttatga tcgacgccag tcggggcttt atcaaagatg
     3061 gcaacaagaa ccgcctgcgt agccaagata ttcaccgtat tgtggacgtg ttcaaccatc
     3121 agcgaacggt gccaggctac agccggatgg tgccctccag cgagatcgcc gggaatgatt
     3181 acaacctgaa tattcctcgt tatatcgaga gtggagagcc ggaagatttg cacgatctga
     3241 ccgcgcactt acaaggcggt attccagtgc gcgatgtaga tgctttgaag gattactggc
     3301 gggtattccc tgccctgcgt aatgtgctgt ttgctgacga ccaccccggt tactgccggg
     3361 cgcaagtgga cgcgcagcag gtgaaaccga ccattctggc tcatcaggag tttaaggatt
     3421 ttgccacccg cagcctgcta ccgtttaagt cgtgggtaaa agaggcaggt ctggaagaga
     3481 tccgcaaagg agataaaccc aaagcgctaa tccacgatat cagcgaaatg ctattggcgc
     3541 agtacgccaa cagtgagttg ctgaacaaat acagcgtgta ccagatcctg atggattact
     3601 gggctgatgt catgcaggac gatgtgtatg tgattatgca ggatggctgg aaagccgccg
     3661 cgcaaatccg cgagcttcag ccggtgaaag gcaaagatgg caaaaacatc tggaaagaaa
     3721 cgcacgattt cgaatttacc aaacggcgct acaaggccga tgtgctgccc cgttcgctgg
     3781 tggaaattcg ctgtttcccg gaactgctgg atgcgctgaa ccgcgcgcag gagcgcagtg
     3841 aagaagccag tcgtcagcta gtggaattca tcgaggagca ggcaggggaa gaggatctgc
     3901 tggctgaggt gaaaaacgat aaagataagg tgacgcaaaa gctggtgaat gcccgtctgg
     3961 cacagttgaa gcccccccct gccgacccgg atgagctggc agtgctgaac cgctgtctgg
     4021 cgctgattaa ggccgaggcc agtgcgaaaa aagcgctgaa agccgcacag gaagcgttgg
     4081 atcgagcggt atttaaacat tatcccacgc tggatgaagc agcgattaaa acgctggtag
     4141 tgcaggataa gtggctggca acgttgcagg cagggatcaa agcggagata gagcgtatta
     4201 cccagcaact ggcgtcgcgc gtaaaggagc tggaagagcg ttatgctgaa ccgttgccag
     4261 cacttgaagc cgccgttgag gcgttgagcg agaaagtggc cgggcatttg cgggcgatgg
     4321 ggctggagtg gtgatggtgg atttaatggt gaatgagcaa cggtcgggta cggcggggaa
     4381 agatgtgcct gcggggtata agctgactga ggtgggggtg attcctgagg attgggtagt
     4441 aatctctatt gatgactgta caaccaaagt tggtagtgga aaaacaccaa ctggtggagc
     4501 atctgtttat attgatagcg gacgcccttt tgtccgtagc caaaatgttg attggggaag
     4561 attagatctc gccgatgtcg tacatattac agatgaaacc cataacacat tctctggttc
     4621 tgaaattcag caagatgatg ttttactcaa cataacggga gcatctatcg gccgatgcgc
     4681 taaagctgat tttagggtta taggtggtaa tgttaaccaa catgtttgca tcatcagaac
     4741 agaggcaaaa aaactctccc catccttact ggttgaatta atcaattcaa attatggaca
     4801 aaaccagatt gatagttatc aagccggagg aaacagagaa ggactgaatt tttctcaagt
     4861 taaaaatctt aagtttgcta ttacaccaaa ctcctttgaa caaacggaaa ttgtcagaac
     4921 attaaatgat attaatgcat ttactctcgc tctggaaaac ttaattgcta aaaaacaggc
     4981 cataaaagcc gctgctatgc aacaactact taccggtaaa acccgtttgc cacagtttgc
     5041 gttgcgcaaa gatggctctg ctaagggata taaaaagagc gagttggggg agattcctga
     5101 agattggaat tgtattaatc ttggtgagct aggtaattgc attattgggt taacttacaa
     5161 acctgaagat acatctgatt atggtacatt ggtattacgc tcatccaaca ttcaaaataa
     5221 tcacttagct tatgatgata acgtcttcgt cagcatagaa ccacctgagc gcacaattgt
     5281 gaaagaaggt gatatcttgg tatgtgtaag aaatggcagt cgccaactta ttggcaaatg
     5341 cgcattaatt gacaaaaatg cgcagggctc agcattcggt gcatttatgt cagtattccg
     5401 aactgatata tatggattta catttcacca attccagtct gatattattc aaaagcaaat
     5461 cgctgaagtt atgggtgcca ctatcaatca gataactaac aaagatatgt tgggctttaa
     5521 aatcccatta cctgaaaata cagaagaaca agccgccatc gccgccatcc tctccgatat
     5581 ggacaaggaa atccagatcc tgcaacagcg tctggacaaa acccgccagc ttaagcaagg
     5641 gatgatgcag gaactgttaa ccgggaaaat ccgcttaata tagaaggaaa agcacatggc
     5701 ttatgattac agcgccagcc tcaacccgca aaaagctctg atctggcgta tcgtccatcg
     5761 ggacaatatc ccgtggattc tggataacgg gctgcactgc ggcaacagtc tggtgcaggc
     5821 ggaaaactgg atcaatatcg gcaacccgga gctgatcggc aagcgtgccg ggcatccggt
     5881 acctgtcgga acgggcggga cactgcatga ctatgtgccg ttttatttta cccccttctc
     5941 gccaatgctg atgaacatcc acagcgggcg cggcggcatt aagcaacgcc ccaatgagga
     6001 gatcgttatt ctggtcagca acttgcgaaa cgtggcagca cacgacgtac ccttcgtgtt
     6061 taccgacagc catgcttact ataactggac caactactac accagtctgg atagtctgga
     6121 ccagatcgac tggcccattt tgcaggcacg ggactttcgc cgcgatccag acgatccggc
     6181 taaatttgaa cgctatcagg cagaggcgct aatctggcaa cactgcccga tctctctgct
     6241 ggatgggata atttgttaca gcgaagaggt caggttacaa ttagaacaat ggctgtttca
     6301 gcgtaatcta acaatgtccg ttcacacacg ggctggatgg tacttctcat gatcacatat
     6361 acacaaggca atttactgga cgcaccggtg gaagctctgg tgaatacggt taataccgtc
     6421 ggtgtcatgg ggaaaggcat cgcgttgatg tttaaagagc gcttccccga aaatatgaaa
     6481 gtctatgccc tcgcctgcaa acaaaagcag gtgatcaccg gcaaaatgtt cattaccgaa
     6541 accggggaac tgatgggacc gcgctggatt gttaattttc cgaccaaaca gcactggcgc
     6601 gctgattccc gcatggagtg gattgaagat ggcttgcagg acttacgccg ctttttgatt
     6661 gaagagaacg tgcagtccat cgccattcca ccactgggcg caggcaacgg tggtttgaac
     6721 tggccggacg ttcgggcgca gattgaatct gcccttggcg accttcagga cgttgatatc
     6781 ctgatttacc aaccgactga aaaataccag aacgttgcca aaagcactgg cgtaaaaaag
     6841 ctcactcccg ccagagccgc catcgctgaa ctggtgcgtc gctactgggt attggggatg
     6901 gagtgcagcc tactggaaat ccagaaactg gcctggctgt tacaacgcgc cattgagcag
     6961 catcaacagg acgatatcct caaactgcgg tttgaagctc actattatgg cccctatgcg
     7021 cccaatctga accatctgct taatgcgctg gacggcacct atcttaaagc cgagaaacgc
     7081 atcccggaca gccaaccgct ggatgtgatc tggtttaacg atcagaaaaa agaacacgtt
     7141 aatgcgtatc tgaacaacga agccagagag tggctacctg cgctggagca ggtcagccag
     7201 ttaattgacg gctttgaatc gccgtttggt ctggagttgc tggcaaccgt agactggttg
     7261 ctgagccgtg gcgaatgtca accaacgctg gattccgtta aggaagggct gcaccagtgg
     7321 cctgcgggcg aacgctgggc cagccgtaag ttgaggctgt ttgataataa taatctgcaa
     7381 tttgccatta accgcgtaat ggagttccat tgctgagggt ttaaggagcg ccttttgagt
     7441 accgtgggcc agcgcgaacg cgccacacag cagcgtctga ttcagttttt tgttgaagat
     7501 ttaggttacc gccatctgag tcaccgcacc gggtacaccg ggaacagcaa catcgacact
     7561 aatatactga gcgcgtggct aaaacggcgc ggcgttaatg atacgttgat cacccgggcg
     7621 ttgcggcaac tggattccgc cgccgctctg ggcgaaggca aaaaactcta ttacgccaat
     7681 aaagaggtct atcgcctact gcgttacggc gtgaaagaca aagaaggcgc aggcgagcag
     7741 aaccaaaccg tgtggctgat tgactgggcc aacccacaag ccaatgattt cgccatcgcc
     7801 gatgaggtga cggtcaaagg cgagtactgc aagcgcccgg atctggtgct gtacgttaac
     7861 ggcattgcgc tgggcgtgat tgaacttaag cgttcgtcag tgcacatcag cgaaggcatc
     7921 cggcagaatc tcgataatca aaagaaaacc tttatccgcg atttcttcac cactatgcag
     7981 ttagtgatgg cgggcaacga tacgcagggc ctgcgctacg gcaccatcga aaccccggaa
     8041 aaatactatc tggagtggaa agaagataat ccaggcgact atacacataa actcgacttt
     8101 catttaagtc gtctgtgtag caaatccagg ctgttggata tcgtccataa ttacattgcc
     8161 tttgatgtcg gggtgaaaaa actctgccgc cataatcagt tcttcggcgt cgaggcggct
     8221 aaacaacata ttgcccgtcg tgaaggcggt attatctggc acacacaggg atcgggtaaa
     8281 agcctgacga tggtctggct ggcgaagtgg atccgtgaaa atgtgccaaa ctcccgcgtg
     8341 ttgattgtga ctgaccgcac cgaactggat gaacagatcg aaagcgtgtt tatgggcgtg
     8401 aatgaagata tttatcgcac ctccagcggc aacgatctta tcgcaacgct caatcatcct
     8461 aacccgtggc tcatttgctc gctggtgcat aaatttggtc gtcgcagcga agctgaagac
     8521 aatgcggcta ccgacgcctt tatcaccgaa ttacagcagt ccctgacgaa aacattccgc
     8581 gccaaaggcg atctgtttgt ttttgtggac gagtgccacc gcacacaatc cggcaagctg
     8641 cataacgcca tgactgccat tctgccggac gccctgttta tcggttttac cggcacaccg
     8701 ctgatgaaga aagataagaa gaagtcggtt gaggtgttcg gcccttatat ccatacctat
     8761 aaatttgatg aagcggtggc cgacggtgtg gtgctggatc tgcgctacga ggcacgcgat
     8821 attgaccagt atctgacatc agagaaaaag gtcgatgact ggtttgaagc caagactcgc
     8881 ggtttgtcga atttagccag gacccagctt aagcaaaagt ggggatcaat gcagaagctg
     8941 ctctccagca agtcgcggct ggagcagatc gttaacgaca ttttgctgga tatggatacc
     9001 cgtccacgcc tgatggacgg gcgcggcaac gccatgctgg tatgcagcag tatctatcaa
     9061 gcctgcaagg tttacgagat gttcagccag accgagctgg caggaaaagt agcgattgtg
     9121 accagctatc gccccgatgc cgccagcatc aaaggcgaag aaacgggcga agggttaacg
     9181 gagaaactct ctaaacacgc cacctatcgt aaaatgttgg ctgactattt tgaacaaagt
     9241 gaagagaagg ccgccgcacg cattaccgag ttcgagcggg aagtgaaaca gcgttttatt
     9301 gaagagccgg ggcaaatgcg cctgcttatc gtggtggata agctgctgac tggctttgac
     9361 gcgccgtccg ccacctatct ctacattgat aaaaccatga ccgaccatac gctgtttcag
     9421 gcgatttgtc gggtgaaccg gctggacggt gaggataaag agtatggcta tattatcgat
     9481 tataaagatc tgttccgttc gctggataaa gcgattaccg attacaccac gggcgcgttt
     9541 gatgattacg atcgggacga tgtggccggg ttgctgaaag acaggctgga gcaggcgcaa
     9601 ctggatctcg acagcgcgct ggagatggtt cgcagcttat gtgaacccgt caaaaccccg
     9661 cgcagcctgc aggactatca gcactatttt tgcggtgaat caggagagaa tcagacttcg
     9721 ttaaacgaaa aagaggcgct gcgcctgaca ttttaccagt gcgtcgcccg tctgctacgc
     9781 gcttatgcca gcatcgccaa tgaaatgccg gaagcaggct acactgccga agaggtagag
     9841 agcattcgcg ccgaagtcgc tgaattcgaa aaactgcgct atgagattaa gctgaccagc
     9901 ggcgatcttc tggatatgaa gcgctttgaa cccgctatgc gtcatttact ggatatgtat
     9961 gttcgcgccg atgatagcga agtgctgatg gattttgaag agttaagtct gattgaactg
    10021 attgtcgaga aaggcttggc ggcgaccgtc gccctgccgg atgatatccg taataatcag
    10081 gatgcgatgg cggaaaccat cgaaaacaac gtgcgcaaaa ccatcgtgga tgaaaacccg
    10141 gttaacccaa aatactatgg acgcatgtcg cagctgctgg atgaactgat tacccttcgc
    10201 cgacaaaatg cgctcaatta tcagcagtat ctggaacgta tccgcgattt gtgtaaacaa
    10261 gtcatccgcc cggagcaaac cgccggagcg tgctaccctg ccaccatgga tacacaagca
    10321 aagcgcgcct tctatgataa cttcggccac gacgaggtac tggcgacccg catagatacg
    10381 acgattcgct ataccaaaag ggcagagtgg attggcgatc gttttaaaga acgggaaatt
    10441 gccaacgcat tacgtgaaga gaccgccagc tataatattg atattgatga ggtgatagcg
    10501 cttgcccgtc agcagaagga atatcactga tgccaatcat gcaaataggc tcgttacact
    10561 tgctggtcaa ccgtaaggcg attaagaatc tgcatatcag cgtgctgccg cctgatggca
    10621 aagtccgcgt ttcagcgccg gaacatatga cggagacggc gattcgtatg gcggttgctt
    10681 cacgctttcg ctggatcaga aaacagcagc aggatttcgc cagacagcct cgtcaatcag
    10741 agagggaaat ggtcagcgga gagtgccact atctttgggg ccgaagatat cgcctacttg
    10801 tgattgagcg tcacgggcgt cacgaagtaa aagttgcggg aaataataag ctccagcttt
    10861 atgttcaacc gggaacccgt gatgaaaata aggcattggc gttaagtgaa ttttatcgac
    10921 atgaactaaa acagcaggtg gctaagcttc tacctgaatg gcaggaccga atcggtgttc
    10981 ccctaacatt ctggggcatc aaaaagatga aaaccttttg gggcacctgt aacactacaa
    11041 caaaacgaat ctggctcaat ctggagctaa ttaagaagcc gccggagtgt ctggagtata
    11101 ttctggtgca tgaactggtt catcttctgg aacgtcacca taatgctcgc ttccggttgc
    11161 atatggatcg gtttattcct gactggcgtg agcgtcggga tttgttaaat aatatgccgt
    11221 tagcgtttga ggcatgggag tattgattga gcctgtaact gttttttgtc cataaattaa
    11281 ccttcatttg atgctggatt gaacatatca aaatcagaca attacacaaa tctatgtaca
    11341 ggctcaactc ctgccatgga acgtagtact caccaataag taagcatcaa tacggcgctc
    11401 cgttaacgct tactagcatg gttatctcct taaaaaattg aggcgacaac tatgctgaca
    11461 cagggggtta ctcaccgatc tccttcacgg taatacgcag ttcttcagaa cccgcctccg
    11521 ataccagcct ggcggtcgcc ccggcgcaac agaataaatc aaccatcgtc agactgcgat
    11581 cattcgtatg catcgctgat gatgcgcccg aaaaacgtgt caatatgagt tcgccggaat
    11641 attaccttac gaaaaaacgt gtaaagctgt gacagatgac gcgcaaaaaa aatcccggca
    11701 tttgcgagct ttcccactat tt
//
LOCUS       DAZKIP010000126        11695 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZKIP010000126 DAZKIP010000000
VERSION     DAZKIP010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.127"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            145..747
                     /gene="lpfA2"
                     /locus_tag="ACHOUK_003842"
     CDS             145..747
                     /gene="lpfA2"
                     /locus_tag="ACHOUK_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA2"
                     /protein_id="HFP9899194.1"
                     /translation="MKPNMIVGALALTSVFMAGHLQAADGTVHFRGEIIDSTCEVTPE
                     TKDQVVDLGKVNRTAFSGVDDVAAPTAFSIDLTQCPETFKSAAIRFDGNEDAHGNGNL
                     AIGTPLDNSNDAAAGISPSDNSGDYTGAGAVSAAKGVAIRLYNRADNTQVKLYENSAS
                     TPISNGNASMKFMARYIATETTIDPGTANADSQFTVEYIK"
     gene            908..1234
                     /locus_tag="ACHOUK_003843"
     CDS             908..1234
                     /locus_tag="ACHOUK_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP9899195.1"
                     /translation="MATAGIVVGGTRVIFYGDKPDTTISIFNKETNLPYLLQTWVDPF
                     NKEDKSKPPFSVIPPVSRIEPQQEKILRILKTAGELPKDRESVFWLNVKNIPPSIDKK
                     IPMFLR"
     gene            1387..1536
                     /locus_tag="ACHOUK_003844"
     CDS             1387..1536
                     /locus_tag="ACHOUK_003844"
                     /inference="COORDINATES: protein motif:HMM:NF014773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9899196.1"
                     /translation="MDDKDVSLNMIRPFEILTLPIPAGVAGKSLVWRFINDYGAISQP
                     LKKNL"
     gene            1558..4092
                     /locus_tag="ACHOUK_003845"
     CDS             1558..4092
                     /locus_tag="ACHOUK_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP9899197.1"
                     /translation="MASSHLFITLASGICLLCSISAFARDSLFNPRLLELDHPADNID
                     IHQFNRSNTLPAGTYKVDVMINGMLFERQEVKFAQDNPDAELHPCYVAIKNVLATYGI
                     KVDAIKSLANVDDKTCVNPVPLIDGATWLLDASKLALNITIPQIYLNNAVNGYISPSR
                     WDQGINAMMMNYDFSASHTIRSNYDDDDDSYYLNLRNGINLGAWRFRNYSTLNSYDGN
                     VDYHSVSNYIQRDIMALRSQIMIGDTWTASDVFDSTQVRGVRLYTDDDMLPSSQNGFA
                     PVVHGIAKTNATVIIKQNGYVIYQSAVPQGAFALTDLNTTSSGGDLDVTIKEEDGSEQ
                     HFIQPFTSLAILKREGQTDVDLSIGEVRDESGFTPEVLQLQAMHGFPLGITLYGGTQL
                     ANDYASAALGIGKDMGALGAISFDVTHARSQFDYDDNESGQSYRFLYSKRFEDTNTTF
                     RLVGYRYSMEGFYTLNEWVSRQDNDSDFWVTGNRRSRFEGTWTQSFTPGWGNIYLTFS
                     RQEYWQTDEVERLLQFGYNNNWRNISWNVSWNYTDSIKRSLGNHHDDNNDDFGKEQIF
                     MFSMSIPLSCWMEDSYVNYSLTQNNHHESTMQVGLNGTMLEGRNLSYNVQESWMHSPD
                     DSYSGNAGMTYDGTYGSVNGSYSWSRDSQHFDYGARGGVLVHSDGVTFSQELGETVAL
                     VKAPGAEGLSIENATGISTDWRGYTVKTQLSPYDENRVALNSDYFSKANIELENTVIN
                     LVPTRGAVVKAEFVTHVGYRVLFNVRQVNGKPIMFGAMATASLETGTVTGIVGDNGEL
                     YLSGMPEKGEFLLSWGQAADEKCKAAYHITHKPDDTSLVQMDAICR"
     gene            4104..5174
                     /locus_tag="ACHOUK_003846"
     CDS             4104..5174
                     /locus_tag="ACHOUK_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9899198.1"
                     /translation="MKKILSGLILLLCCPYGFAANGDGATHMSNLSFGPLTVAAANNH
                     SGYNIFEALSNTTGTYPVRCHCDDTHGGPGQQTAFFPIFYTGDAAPGLVLERTLNGLN
                     YYALNDYLSVGVTIFIINNQYAAIPFEHLSNQSTSPQHTCGAGNNGSTVNLDSGRSAK
                     LSFYVRHSITGTVTIPTTEVAWLYAGMSDHFPKTTPVSKVTIRGQLTAPQNCELTPNQ
                     SIDVDFQKINSAEFSSTAGSIIAERKIKTEVTVSCTGMEDVRSTEVVSASMIAANRSA
                     DATMIVTSNPDVGIKIFDKNDRPVNVDGGNLPADMGAISRLGKTDGSVTFYSAPASLT
                     GAKPAPDNGFTATATLVIEFTN"
     gene            5202..6284
                     /locus_tag="ACHOUK_003847"
     CDS             5202..6284
                     /locus_tag="ACHOUK_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9899199.1"
                     /translation="MKKWHYIFCIILFHLGLPCGYAANDGTCATRGGTHTLSLNFPLT
                     TVSAANNVPGNTLIDIANATSSENYSVLCNCDSKHSNGAYHEIYYTADPAPGMVYSTT
                     ASGLAFYYLNEYVDVGTKISVLNAGYTAVPFEHVSNQATTTDHTCQGNKTTAVGVSLK
                     TGADAKISFRIKRSINGTVVIPITDIALLYANISSTTTRGEAIAKVRISGSLTAPQSC
                     QINAGQVIYFDFDTIPASEFSSTAGQAITSRKITKTVSIECTGMGYERTQKVDASFTG
                     TNRSSDDTMVATDNADVGIKIYNKSNAEVSVNNGKLPADMGNTTIFGRKNGSVTFSAA
                     PASFTGARPQPGVFNATATLTIEFVN"
     gene            6496..7536
                     /gene="pstS"
                     /locus_tag="ACHOUK_003848"
     CDS             6496..7536
                     /gene="pstS"
                     /locus_tag="ACHOUK_003848"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFP9899200.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVLPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            7623..8582
                     /gene="pstC"
                     /locus_tag="ACHOUK_003849"
     CDS             7623..8582
                     /gene="pstC"
                     /locus_tag="ACHOUK_003849"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFP9899201.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            8582..9472
                     /gene="pstA"
                     /locus_tag="ACHOUK_003850"
     CDS             8582..9472
                     /gene="pstA"
                     /locus_tag="ACHOUK_003850"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFP9899202.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            9655..10428
                     /gene="pstB"
                     /locus_tag="ACHOUK_003851"
     CDS             9655..10428
                     /gene="pstB"
                     /locus_tag="ACHOUK_003851"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFP9899203.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            10443..11168
                     /gene="phoU"
                     /locus_tag="ACHOUK_003852"
     CDS             10443..11168
                     /gene="phoU"
                     /locus_tag="ACHOUK_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFP9899204.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDPKE"
     gene            11593..>11695
                     /locus_tag="ACHOUK_003853"
     CDS             11593..>11695
                     /locus_tag="ACHOUK_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_307391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899205.1"
                     /translation="MAISSYSTVTDKFANLTKMPDENNGLQKNQKNFV"
BASE COUNT         3064 a         2707 c         2890 g         3034 t
ORIGIN      
        1 atctaaatgt ataaaataaa aaccacttgc gccaaagtat attggtagta ctatttggtt
       61 tctgatcaaa taccgatcag gtttatccaa atttaataaa tggataacac acaggaataa
      121 tatcaaatta atggagttat atttatgaaa ccaaatatga ttgtaggagc attagcgtta
      181 acttctgtgt ttatggcagg tcacctacag gcggctgatg gaacagtcca tttccgtggt
      241 gaaattattg acagtacttg cgaagtcact cctgaaacta aagatcaggt cgttgattta
      301 ggcaaagtaa accgtacagc ctttagtggc gtcgatgatg tggctgcccc gacggctttt
      361 tctatcgatc tgactcaatg cccggaaacc tttaagtccg ccgcaattcg tttcgatggt
      421 aatgaagatg ctcatggtaa tggcaacctg gcaattggta ccccgctgga taactctaac
      481 gatgctgccg ctggtattag cccgagtgat aacagtgggg attatactgg tgcgggtgcc
      541 gttagtgcag cgaaaggcgt agctattcgt ttatataacc gtgcagataa cactcaggtc
      601 aagttatatg aaaattctgc atcaactccg atttctaatg gtaatgcatc catgaagttc
      661 atggctcgtt atattgctac ggaaacgact attgaccctg gtacagctaa cgccgactcg
      721 cagtttacag ttgaatatat aaaataaatc attcttaaca taatacctta aataaaatgg
      781 cgctcgccat tatggttctc ttttacttaa atgaagggcg tcattattat attttttatg
      841 agatacattt atgcctatgt atttacttag attgtttttt gctgttactt tgattacgca
      901 gatgtcgatg gctacggctg gtattgttgt tgggggaact cgcgttattt tttatggtga
      961 caaacctgat accacaatct ctatttttaa taaagaaacg aaccttcctt atctattgca
     1021 gacatgggtt gatccattta ataaagagga taaaagcaaa ccgccatttt ctgtgatacc
     1081 tcccgtttct cgcattgaac cacagcaaga gaaaatatta cgcattttaa aaacggctgg
     1141 cgagttacca aaagatcggg agtccgtatt ctggttgaac gtgaaaaaca tccctccttc
     1201 aattgataaa aaaataccaa tgttcttgag atagccataa aaacaagaat taagttattc
     1261 tggcgaccag ccgcggtaaa gataatccct gagcaggcta ttaaaaatgt taaatggtcg
     1321 cggcagggta ataatctcat cgtcgaaaat ccaaatccga tccatattaa tgtaatgagt
     1381 gtgacggtgg atgataaaga cgtgtcgcta aatatgatcc gcccttttga aatattgaca
     1441 ttaccaattc cggctggcgt ggcaggaaaa tcgctcgttt ggcgctttat taatgattac
     1501 ggtgcaatta gtcaaccact aaagaaaaac ctttaagcgc cggataagga tatatcgatg
     1561 gcctcttcgc atcttttcat tacgcttgca tcgggcatat gtctgctctg ttccatatct
     1621 gcttttgccc gggatagctt gttcaacccc agattactgg aactggatca tcctgcggat
     1681 aatattgata ttcaccagtt caaccgttcg aataccttac ctgcgggaac atacaaagtt
     1741 gatgtgatga tcaacggcat gctcttcgaa cgccaggaag ttaaattcgc ccaggataac
     1801 cctgatgctg aactccaccc atgctacgtg gcgataaaaa acgtgctggc gacctatggt
     1861 ataaaagttg atgcgataaa atctctggcg aatgttgatg acaaaacatg cgtaaatcca
     1921 gttccgctga tcgacggggc tacctggtta ctggacgcca gtaaacttgc attgaatatt
     1981 actattccgc aaatttatct caacaatgca gttaatggtt atatcagccc ttcccgttgg
     2041 gatcagggga tcaatgccat gatgatgaat tatgattttt cggcatcgca taccatccgg
     2101 tcaaattatg acgacgacga tgacagttat tatctgaatt tgcgtaatgg tattaattta
     2161 ggcgcatggc gttttcgtaa ttacagcacc ctgaattctt atgacggtaa tgtggactac
     2221 cattccgtca gtaattacat tcagcgcgac atcatggcat tacgtagcca gattatgatt
     2281 ggcgatacct ggacggcaag cgatgtattt gatagtacac aggtgcgtgg cgtgcggctg
     2341 tataccgatg acgatatgtt gccctccagc cagaacggct ttgcgccagt ggtacatggg
     2401 attgcgaaaa ctaacgccac ggtgatcatc aaacaaaacg gctacgttat ttatcaatca
     2461 gccgtaccac agggcgcatt tgccctcacc gacttaaaca cgaccagtag cggcggcgat
     2521 ctcgatgtca ctatcaaaga agaagatggc agcgagcagc actttattca gccatttact
     2581 tcactggcca ttctcaagcg tgaaggtcag accgatgtag accttagcat tggagaagtg
     2641 cgcgacgaaa gcggctttac gcctgaggtc ttgcagttac aagcaatgca cggtttccct
     2701 ttgggaataa ctttgtatgg cggaacacaa ttggcaaatg attacgcttc tgccgcgctg
     2761 ggtattggta aagatatggg ggcgctgggc gcgatttctt ttgacgtgac tcatgcccgc
     2821 tcgcagtttg actacgacga taatgagagt ggtcaatcgt atcgttttct ctattccaaa
     2881 cgttttgaag acaccaatac cacctttcgt ctggtgggtt atcgctactc tatggagggg
     2941 ttctacaccc tcaatgaatg ggtgtcgcga caggataatg attctgattt ctgggtaacg
     3001 ggcaaccgtc gcagccgctt cgaaggcacc tggacgcaat ctttcacgcc aggctggggc
     3061 aatatttatt taacattcag tcgacaggaa tactggcaga ccgatgaggt cgaacgttta
     3121 ttacagttcg gctataacaa caactggcga aacatctcct ggaacgtttc ctggaactat
     3181 acggactcga tcaagcgctc attgggcaac catcatgatg ataacaatga tgatttcggc
     3241 aaagaacaga ttttcatgtt ctcaatgtcg ataccgctat cgtgctggat ggaagacagc
     3301 tacgtcaact attcgttaac gcaaaacaac caccatgaaa gcacgatgca ggtcggtctg
     3361 aacggaacga tgctggaagg gcgtaacctg tcttataacg tacaggaatc gtggatgcac
     3421 tctcctgatg actcctacag cggcaatgcc ggaatgacct atgacgggac ttatggctcg
     3481 gtcaatggta gctattcctg gagccgtgac tcccaacatt ttgattatgg cgccagaggc
     3541 ggcgtgctgg tgcatagtga cggagtgacc ttctcgcagg aactgggcga aacggtggca
     3601 ttggtcaaag cgccgggcgc agaaggcctg tccattgaaa acgccaccgg gatttctacc
     3661 gactggcgtg gttataccgt aaaaacgcag cttagcccgt atgacgaaaa ccgcgtggca
     3721 ttgaacagcg actatttctc caaagccaat attgaactgg aaaacaccgt catcaacctg
     3781 gtaccaacgc gcggtgcggt ggtgaaagcc gaatttgtca cccatgtcgg ttatcgcgtg
     3841 ctatttaacg tccgccaggt caacggtaaa ccaataatgt ttggcgcgat ggcaaccgcc
     3901 tctctcgaaa cgggcacagt caccgggatt gtcggtgata acggcgaact gtatctctcc
     3961 gggatgcctg aaaaaggcga gtttttattg agttggggac aagctgcgga tgaaaaatgt
     4021 aaggcggcct atcacatcac ccataaacct gatgatacca gcctggttca aatggatgcg
     4081 atttgtcgct aaaggaagca tgaatgaaaa aaatattatc agggttgatt ctgctgcttt
     4141 gctgtcctta tggtttcgcc gctaatggtg atggcgcaac gcacatgtca aatttatcat
     4201 ttggtccgct gacggtggca gcggcgaata accactccgg atacaatatt ttcgaggcac
     4261 tgagcaacac gactggaaca tacccggtgc gctgtcactg tgatgacacg catggcggac
     4321 cgggccaaca aacagcattt tttcctatct tctacacggg agatgccgca ccggggcttg
     4381 tgcttgagcg cactcttaat gggttaaatt actatgctct gaatgattat ttatcggtcg
     4441 gcgtgacgat ttttattatt aataaccaat atgcggccat tccttttgaa cacttatcca
     4501 accaatccac ctcaccgcaa catacctgtg gagcaggtaa taatgggagc actgtaaatc
     4561 tggattcagg gcgctcggca aaattatctt tctatgttcg gcattctatt actggcacgg
     4621 tgacaatacc cacaacggaa gtcgcctggt tgtacgcggg catgtccgat cattttccca
     4681 aaacgacccc cgtttctaaa gtgacaattc gcggacaact aacggccccg cagaactgtg
     4741 agttaacgcc aaatcagagc atcgatgtcg attttcaaaa aattaatagc gctgagttct
     4801 cctcaacggc gggttcaatt attgcagaaa gaaagattaa aaccgaagtc acagtatcct
     4861 gtaccgggat ggaagacgta aggtccacgg aggtggtgag tgcgtcgatg attgcggcaa
     4921 acagaagtgc cgatgccacc atgatcgtga cgagtaatcc ggatgtggga attaagattt
     4981 ttgataagaa cgaccgtcca gtgaatgtgg atgggggcaa cttacccgct gatatgggtg
     5041 ctattagtcg attaggaaaa accgatggta gcgtaacgtt ttattcagcg cccgccagtc
     5101 tgacgggcgc aaaaccagcg cctgataatg gatttaccgc tacagccacg ctggttattg
     5161 aatttactaa ctaatcttgt gttagtaaaa ggaataattt catgaaaaaa tggcattata
     5221 tattttgcat aattctcttt catttagggt taccgtgcgg gtatgcggca aatgatggca
     5281 cgtgtgcaac aagaggcggc acacatacat taagccttaa ttttcctctg acaacggtca
     5341 gtgcagcaaa caatgtgcct ggaaatacat taatagatat tgctaatgca acatcttctg
     5401 aaaattatag cgttctgtgt aactgtgatt caaaacatag caatggcgct tatcacgaaa
     5461 tatattatac cgcagaccct gctcccggta tggtttatag caccaccgca agtggtcttg
     5521 ctttttacta tcttaacgaa tatgtcgatg tgggaacaaa aatatctgtg ctaaatgcgg
     5581 ggtatacggc agttcctttt gaacatgttt ccaaccaggc aactacaaca gatcacactt
     5641 gtcagggaaa caaaactaca gcggttggcg tgagcctgaa aactggagca gatgcgaaga
     5701 tttcatttcg tattaaacgt tcaataaatg gaacggtagt aatacctatc accgatattg
     5761 cattgctgta tgccaacata tccagcacca cgacccgtgg tgaggcgatt gcaaaagttc
     5821 gaatttcagg cagtttgacc gcaccacagt cttgtcagat aaatgcagga caggtgattt
     5881 attttgattt tgatactatt cctgcgtccg aattttcatc taccgccggg caagccatta
     5941 cttcacgaaa aatcactaaa acagtgagta ttgagtgtac ggggatgggg tatgagcgta
     6001 cgcagaaagt cgatgcttct tttacgggga cgaaccgaag cagtgacgat acgatggtgg
     6061 cgacagacaa tgctgatgtc gggatcaaaa tttacaataa atcgaatgct gaagttagcg
     6121 tcaacaacgg caagttaccc gcagacatgg gcaacacgac catttttggt cgtaaaaatg
     6181 gttcggtaac tttttcggca gcacctgcca gctttaccgg tgcccggcct cagcccggcg
     6241 tttttaacgc taccgcgacc ttaaccattg aatttgtaaa ctaacctttt gcggctgttt
     6301 tatgacagcc gttcatattt cctctccttt catgaaaaaa tgctgatatt tttccccttc
     6361 aatatcaata agtaataact ttcctttggg tgtcatcaaa ctgtcacatt ccttacatat
     6421 aactgtcacc tgtttgtcct attttgcttc tcgtagccaa caaacaatgc tttatgaatc
     6481 ctcccaggag acattatgaa agttatgcgt accaccgtcg caactgttgt cgccgcgacc
     6541 ttatcgatga gtgctttctc tgtgtttgca gaagcaagcc tgacaggtgc aggtgcaacc
     6601 ttccctgcgc cggtgtatgc caaatgggct gacacttacc agaaagaaac cggtaataaa
     6661 gttaattacc agggtatcgg ttcttccggt ggcgtaaaac agattatcgc caataccgtt
     6721 gattttggtg cttctgacgc gccgctgtct gacgaaaaac tggctcagga aggtctgttc
     6781 cagttcccga ccgtgattgg cggcgtggtg ctggcggtta acattccagg tctgaagtct
     6841 ggtgagctgg tgctggatgg taaaaccctc ggcgatatct acctgggcaa aatcaagaag
     6901 tgggatgatg aagccatcgc caaattgaat ccgggtctga aactgccttc acagaacatt
     6961 gctgtagtgc gccgcgcaga tggctccggg acttccttcg tcttcaccag ctacctggcg
     7021 aaagtgaatg aagagtggaa aaacaacgtt ggtactggct ctaccgtaaa atggccgatc
     7081 ggtctgggtg gtaaaggtaa cgacggtatc gctgcgttcg ttcagcgtct gccgggtgca
     7141 attggttacg ttgaatatgc ttatgcgaag caaaacaacc tggcgtacac caaactgatc
     7201 tccgctgatg gtaaacctgt tttgccgacc gaagaaaact tcgctaatgc agcaaaaggt
     7261 gcagactgga gcaaaacctt cgctcaggat ctgaccaacc agaaaggcga agatgcgtgg
     7321 cctattacct ctaccacgtt cattctgatc cacaaagatc agaagaaacc agaacaaggc
     7381 acagaagtgc tgaaattctt cgactgggcg tacaaaaccg gggctaaaca ggcgaacgac
     7441 ctggattacg ccagcctgcc ggatagcgta gttgaacagg ttcgcgctgc gtggaagacc
     7501 aatattaaag acagtagcgg caagccgctg tactaataaa actccaggcc ggataaggca
     7561 ttaaacggct tatccggcat tacaaaatga ctttgtaaac gcgtttaact gaagagtaac
     7621 ttatggctgc aaccaagcct gcttttaacc caccgggtaa aaagggcgac ataattttca
     7681 gcgtgctggt aaaactggcg gcgctgattg tgctattgat gttgggtggc attattgtct
     7741 ctctgatcat ctcctcatgg ccgagcattc agaaatttgg tctggctttc ctgtggacca
     7801 aagagtggga tgcgccgaac gatatctacg gggcgctggt gccgatctac ggtacgttgg
     7861 tgacttcgtt tatcgcgctg ctgatcgccg tcccggtgag tttcggtatc gccctgttcc
     7921 tgactgagct tgcgcctggc tggctgaaac gcccgctggg tatcgccatt gagctgctgg
     7981 cagccattcc aagtatcgtt tacggcatgt ggggcctgtt tatctttgcg ccgctgttcg
     8041 ccgtttactt ccaggagccg gtcggcaata tcatgtcgaa tatcccgatt gtcggcgcgc
     8101 tgttctctgg cccggcattt ggtatcggta tcctcgcggc aggcgtgatc ctcgccatca
     8161 tgattattcc gtacattgcg gcggtaatgc gcgatgtgtt cgaacaaacc ccggtgatga
     8221 tgaaagagtc ggcctacggt attggctgca ccacctggga ggttatctgg cgtatcgttc
     8281 ttccgttcac caaaaatggt gttatcggcg gcattatgct ggggctgggc cgcgcgcttg
     8341 gtgaaaccat ggcggtgacc tttattatcg gtaacaccta ccagctcgac agcgcctcgc
     8401 tgtacatgcc gggcaacagt atcacctctg cgctggcgaa cgaatttgcc gaagcggaat
     8461 ccggtctgca cgttgccgca ctgatggaac tgggcctgat cctgtttgtg attaccttca
     8521 tcgtcctcgc cgcatcgaag tttatgatta tgcgcctggc taagaatgag ggggctcgct
     8581 aatggctatg gttgaaatgc aaaccactgc ggcgctggcg gaatcccgcc gcaaaatgca
     8641 ggcgcgtcgc cgcctcaaaa accgtattgc actgacgctc tcaatggcga cgatggcctt
     8701 cggtctgttc tggctgatct ggattttaat gtccaccatc acccgcggta tcgacggtat
     8761 gtcgctggcg ctgttcactg aaatgacgcc gccgcccaat acggaaggtg gtggtctggc
     8821 gaacgctctg gcaggtagcg ggctgttaat tttgtgggcc acggtattcg gtacgccgct
     8881 gggcattatg gcggggattt atctggctga atatggtcgt aaatcctggc tggcagaagt
     8941 gattcgcttc attaacgaca ttctgctctc tgcgccgtcg attgtggttg gtctgtttgt
     9001 ttacaccatc gtggtggcgc agatggagca cttctccggt tgggcgggtg tgattgcgct
     9061 ggcgttgttg caggtgccga ttgttatccg caccaccgag aacatgctga aactggtgcc
     9121 ggacagcctg cgtgaagcgg cttatgcgct gggtacaccg aagtggaaga tgatctccgc
     9181 gattacgctg aaagcgtcgg tgtccgggat catgaccggt atcctgctgg cgattgcccg
     9241 tattgctggt gaaaccgcgc cgctgctgtt taccgcgctc tccaaccagt tctggagcac
     9301 ggacatgatg cagccgatcg ccaacctgcc ggtgacgatc tttaagtttg cgatgagccc
     9361 gtttgcggaa tggcagcaac tggcctgggc cggggtattg atcattaccc tgtgcgtact
     9421 gctgctgaac attctggcgc gcgttgtttt cgcgaagaat aaacacggtt gatattgctg
     9481 acacggtttt cccctcaccc taaccctctc cccagagggg cgaggggacc gaccgagcgc
     9541 ctttttgact ctgtacacgg ttaacacttt gccggatgcg gcgtgaacgc ctgatccggc
     9601 ctacggtaag cctgattcgc gaagtgcatc aggcacgatg aggaaaagat tgcaatgagt
     9661 atggttgaaa ctgccccgag taaaattcag gttcgtaatt tgaacttcta ctacggcaaa
     9721 ttccatgccc tgaaaaacat caacctggat atcgccaaaa accaggtaac ggcgtttatc
     9781 gggccgtccg gctgcggtaa atcgacgctg ctgcgtacct tcaacaaaat gtttgaactg
     9841 tacccggaac agcgtgcgga aggtgaaatt ctgctcgatg gcgacaacat cctgaccaac
     9901 tctcaggata tcgccctgct gcgtgcgaaa gtgggcatgg tgttccagaa accgacgccg
     9961 ttcccgatgt ccatctacga caacatcgcc tttggcgttc gtctgtttga gaagctctct
    10021 cgtgccgaca tggacgagcg cgtgcagtgg gcattgacca aagccgcatt gtggaacgaa
    10081 accaaagata aattgcatca gagcggttac tctctctctg gtggtcagca acagcgtctg
    10141 tgcattgcgc gtggtatcgc cattcgcccg gaagtgctgc tgctcgacga accatgttcg
    10201 gcgctcgacc ccatctccac cgggcgtatt gaagagctga tcaccgaact gaagcaggat
    10261 tacaccgtgg tgatcgtcac ccacaacatg cagcaggctg cgcgttgttc cgaccacacg
    10321 gcgtttatgt acctgggcga attgattgag ttcagcaaca cggacgatct gttcaccaag
    10381 ccagcgaaga aacaaacaga agactacatc accggtcgtt acggttgatt caggagtgcg
    10441 ttatggacag tctcaatctt aataaacata tttccggcca gttcaacgcc gaactggaaa
    10501 gtatccgcac gcaggtgatg accatgggcg gcatggtgga gcagcagctt tctgatgcaa
    10561 tcaccgcgat gcataaccag gacagcgatc tggcgaagcg cgtgatcgaa ggcgacaaga
    10621 acgtcaacat gatggaagtg gcgatcgatg aagcctgcgt gcgcattatt gccaaacgcc
    10681 agccgacggc gagcgacctg cgattggtta tggtgatcag taaaaccatt gccgaactgg
    10741 agcgtattgg cgacgtggcg gacaaaatct gccgtactgc gctggagaaa ttctcccagc
    10801 agcatcagcc gttgctggta agtttggagt cgctgggccg tcatactatc cagatgctgc
    10861 acgacgtgct ggacgcgttc gcgcggatgg acattgacga agcggtacgt atttatcgtg
    10921 aagataaaaa agtcgatcag gagtacgaag gtattgttcg tcaactgatg acctacatga
    10981 tggaagattc gcgcaccatt ccgagtgtac ttaccgcgct gttctgtgcg cgttctatcg
    11041 aacgtattgg cgaccgctgc cagaatattt gtgagtttat cttctactac gtgaagggtc
    11101 aggatttccg tcatgtaggc ggtgatgagc tggataaact gctggcggga aaagacccca
    11161 aagagtagtg ctaactgtag gccagataaa gctttttgcc gcatctggca atcagcccgg
    11221 tcatacatag ttaccgggct gatacacttt ctcactgaaa tattgccaca caaactcacc
    11281 tcgaaactct ccccagtaca ttgaatatac attatttcat ttgaattatt ttacttttaa
    11341 tgtagtgcct cgctataaaa acatatttgt tattcttttc atttatatcg ctacctgcac
    11401 agagaatgga aaagaaaaca aaaaacaggg taaaagccct atatcgagac ataaaaaatg
    11461 agcgattttt tacagtattc ataggtggtt tagtcataat cgtagcaaac caacacccag
    11521 gtatgctttt taagacaaat cggcaatgta ttatgctcgg gcgaataaat attgtgttca
    11581 ggagaaatga ttatggctat atcatcatat agtacagtta cagataaatt tgctaatctg
    11641 actaaaatgc cagatgaaaa taatggattg caaaaaaatc agaaaaattt tgttt
//
LOCUS       DAZKIP010000127        11502 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZKIP010000127 DAZKIP010000000
VERSION     DAZKIP010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.128"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            245..1015
                     /gene="yjjP"
                     /locus_tag="ACHOUK_003854"
     CDS             245..1015
                     /gene="yjjP"
                     /locus_tag="ACHOUK_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313351.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFP9899206.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQHPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1006..1479
                     /gene="yjjB"
                     /locus_tag="ACHOUK_003855"
     CDS             1006..1479
                     /gene="yjjB"
                     /locus_tag="ACHOUK_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFP9899207.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1586..2125
                     /gene="dnaT"
                     /locus_tag="ACHOUK_003856"
     CDS             1586..2125
                     /gene="dnaT"
                     /locus_tag="ACHOUK_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFP9899208.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVDLDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2128..2865
                     /gene="dnaC"
                     /locus_tag="ACHOUK_003857"
     CDS             2128..2865
                     /gene="dnaC"
                     /locus_tag="ACHOUK_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFP9899209.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2914..3408
                     /gene="yjjA"
                     /locus_tag="ACHOUK_003858"
     CDS             2914..3408
                     /gene="yjjA"
                     /locus_tag="ACHOUK_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFP9899210.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDVENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3662..5953
                     /gene="opgB"
                     /locus_tag="ACHOUK_003859"
     CDS             3662..5953
                     /gene="opgB"
                     /locus_tag="ACHOUK_003859"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFP9899211.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6090..7112)
                     /gene="lgoD"
                     /locus_tag="ACHOUK_003860"
     CDS             complement(6090..7112)
                     /gene="lgoD"
                     /locus_tag="ACHOUK_003860"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFP9899212.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITAGMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7251..8165
                     /gene="lgoR"
                     /locus_tag="ACHOUK_003861"
     CDS             7251..8165
                     /gene="lgoR"
                     /locus_tag="ACHOUK_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFP9899213.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8380..9741
                     /gene="lgoT"
                     /locus_tag="ACHOUK_003862"
     CDS             8380..9741
                     /gene="lgoT"
                     /locus_tag="ACHOUK_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9899214.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9790..11454)
                     /gene="tsr"
                     /locus_tag="ACHOUK_003863"
     CDS             complement(9790..11454)
                     /gene="tsr"
                     /locus_tag="ACHOUK_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFP9899215.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
BASE COUNT         2788 a         3049 c         2802 g         2863 t
ORIGIN      
        1 ccagctatct attatcctga cccgtaaagg ctaaaacaat aaattcatta tagtgataat
       61 attcatgtga tagtttgttt gcggcgagag ataattcgct ttttatcacc gagcttgtcc
      121 cgataaccag gattaatcag cgcaattttg atcttaacct accgcaaaac aaccttgctt
      181 catgatacaa atggattctc attatctgaa caatacgcag cacgtctatg acaaagggcg
      241 agttatgcaa actgagcaac agcgagccgt aacacggctt tgtatccagt gtggattatt
      301 tcttttgcaa catggtgcgg aaagcgcgtt ggttgatgag ctttcctcac gactgggtcg
      361 ggcgctggga atggacagcg tcgaaagttc tatctcttcg aacgccatag tgctgacaac
      421 tattaaagat gggcaatgcc tgacatcgac acgtaaaaat cacgatcgcg gcattaatat
      481 gcatgtggtg actgaagtcc agcacattgt gattcttgcg gagcatcatc tgctggatta
      541 caaaggcgta gagaaacgat ttagccaaat tcagccatta cgttacccaa gatggctggt
      601 agccttaatg gttggccttt cttgcgcctg tttctgtaaa ctcaataacg gtggctggga
      661 tggtgccgtc atcaccttct ttgccagtac gaccgcgatg tatatccgcc agctgctggc
      721 acaacgtcat cttcatccac agatcaactt ttgccttacc gctttcgccg ccaccaccat
      781 ttccggattg cttttgcaac accccacttt cagcaatacc cccaccattg cgatggccgc
      841 cagcgttctg ctgctagtgc cgggctttcc gttgattaat gccgtcgccg atatgtttaa
      901 aggccacatc aataccggac tggcacgctg ggcgatcgcc agtctgctga cactggctac
      961 ctgcgtcggc gtagtgatgg cactgacgat ttgggggcta cgcggatggg tgtgatcgaa
     1021 tttctgttag cgttggcgca ggatatgatc ctcgccgcca ttcctgcggt cggctttgcg
     1081 atggtgttca acgttcccgt gcgggcgtta cgctggtgtg cgctgcttgg ctcgataggt
     1141 catggttccc gaatgatctt gatgaccagc gggttgaata ttgagtggtc aacctttatg
     1201 gcctccatgc tggtcggcac cattggtatt caatggtcgc gctggtatct ggcgcatccg
     1261 aaagtgttta ccgtggcggc cgttatccct atgttcccgg gcatatcggc ttataccgca
     1321 atgatttcgg cggtaaaaat cagccagtta ggttacagcg aaccgttgat gattaccctg
     1381 ttaaccaact ttcttacagc ttcatcgatt gttggtgcgt tatccatcgg tctttccatt
     1441 cctggattat ggttgtaccg caagcgccct cgcgtataaa attgcctcgc ttaagcacac
     1501 ggatgagaga cagcctcctc tcctccgtgt gttactataa aagttatctc ccttctcgtt
     1561 catcgttcca tatttgagaa acagtatgtc ttccagagtt ttgaccccgg acgtcgttgg
     1621 tattgacgcc ctggtacacg atcaccaaac cgttctggca aaagctgaag gcggtgtggt
     1681 tgccgtattt gctaacaatg ccccggcgtt ttatgccgtc acgcctgcac gcctggctga
     1741 actgctggcg ctggaagaaa agctggcgcg tccgggaagc gatgtcgatc tggacgatca
     1801 actctatcag gaaccgcaag ccgctcccgt tgctgtaccc atggggaaat tcgccatgta
     1861 tccggactgg caacccgatg ccgactttat ccgcctggcg gctctatggg gcgtggcgct
     1921 aagagagccg gtaaccaccg aagaactggc ctcattcatt gcctactggc aggcggaagg
     1981 taaagtcttt caccatgtgc agtggcaaca aaaactggcg cgcagcctgc aaatcggtcg
     2041 tgccagcaac ggcggactgc cgaaacgaga tgtgaatacg gtcagtgaac ctgacagcca
     2101 aattccacca ggattcagag ggtaacgatg aaaaacgttg gcgacctgat gcaacgcctg
     2161 caaaaaatga tgcctgccca tatcaaacct gcattcaaaa cgggtgaaga gctactggcc
     2221 tggcagaaag aacaaggggc gatccgctcc gccgctctcg aacgtgaaaa tcgggcgatg
     2281 aaaatgcagc gcacctttaa ccgctccggt attcgcccac tgcatcagaa ctgctccttt
     2341 gagaactatc gcgttgagtg tgaagggcag atgaatgcgt taagcaaagc gcgccagtat
     2401 gtcgaagagt ttgacggcaa catcgccagc ttcatctttt ctggtaagcc aggaaccggc
     2461 aaaaaccatc tggcggcggc aatctgcaac gagctgctgc tacgcggtaa atccgtattg
     2521 atcatcaccg tggccgatat tatgtcggcg atgaaagata ccttcaggaa tagcggtacc
     2581 agcgaagaac aactgcttaa cgatctgagc aacgttgatc tgctggtgat cgatgagatc
     2641 ggcgtgcaga ccgaatcgaa atacgaaaaa gtgatcatca accagatcgt cgatcgccgc
     2701 tcttcttcca aacgcccaac cgggatgctg accaacagta atatggaaga gatgaccaag
     2761 ttgctgggcg aacgcgtgat ggaccgtatg cgcctgggta acagtttgtg ggtgatcttc
     2821 aactgggata gctaccgtag ccgtgtaaca ggtaaagagt attaatcccc gtttctggcg
     2881 gtatactgcg acacaattcc accttgagta atgatgatga aaactgttaa acatcttctg
     2941 tgctgtgcca ttgccgccag cgcattaatt tccaccgggg tgcatgctgc gtcctggaaa
     3001 gatgcgctct ccagcgccgc cagcgaactt ggcaaccaaa acagcacgac acaggaaggc
     3061 ggttggtcgc tcgcgtcatt aactaacttg cttagcagcg gaaaccaggc cttaagcgca
     3121 gataacatga acaatgccgc aggcattctg caatactgcg cgaagcaaaa gctggcttcg
     3181 gtaaccgatg tcgaaaacat caagaaccag gtgctggaaa agctgggcct gaacagtgaa
     3241 gagcaaaaag aagacaccaa ctatctggac ggcattcagg gtttgctgaa aaccaaagat
     3301 ggtcagcaac tcaatctgga taacatcgga acaactccgc tggcagaaaa ggtgaaaacc
     3361 aaagcctgcg atctggtgtt aaagcagggg ctgaacttca tttcctgaac cgcgaaacgc
     3421 cgcgttttag ctgttacccg gctgacgcgg ctctctcctg cccttttccc tgctcctgga
     3481 cggttttccc cctgttacac gggaagcgac cgcgatcaaa aaagtctgtt tataaactaa
     3541 ttcctaacaa catcgcattt ttttgacact agaattgcag caatatggcg acgccattac
     3601 attgtgcttc tcaaaaagct taaacgttgg ctaaacggcc cggtttagtg aggatcgccc
     3661 gttgtcagaa ctactctctt tcgccctttt tctcgcctct gtgctgattt acgcatggaa
     3721 agcgggacgt aacacctggt ggtttgcagc cacattaacg gtgctggggc tatttgtcgt
     3781 tttaaatatc accctgtttg ccagcgacta ttttactggc gatggtatta acgacgcggt
     3841 tctctatacc ttaaccaaca gcctgaccgg tgctggcgtc agcaaataca ttttgccggg
     3901 tatcggcatt gtgctggggc tgacagcggt gttcggtgcg ctgggctgga tcctgcgccg
     3961 tcgtcgccat catccgcacc attttggtta cagcctgctg gcgctcttac tggcgctggg
     4021 ttcggtggac gccagcccgg catttcgtca gataacggaa ctggtgaaat cccagtcacg
     4081 cgacggcgac ccggactttg cggcttatta taaagagccg tcgaaaacta tccctgaccc
     4141 gaaactcaac ctggtttata tctacggcga aagtctcgag cggacctatt ttgataacga
     4201 ggctttcccg gacctcacgc ctgaactggg cgcgttgaaa aatgaaggcc tggatttcag
     4261 ccacacgcag cagctgccgg gaacagatta cacgattgcg ggcatggtgg cttctcagtg
     4321 cggcataccg ctgtttgccc cctttgaagg caacgcctcc gcttctgttt ccagcttctt
     4381 cccgcagaac atctgtctgg gcgatatcct gaaaaactcg ggttatcaga actatttcgt
     4441 gcagggcgcg aatctgcgtt ttgccggtaa agatgtgttc ctgaagtcgc atggcttcga
     4501 ccacttatac ggctcagaag agctgaaaag cgtggtagcc gacccgcact atcgcaacga
     4561 ctggggattc tacgacgata ccgttctcga tgaagcgtgg aaaaagtttg aagagctttc
     4621 ccgctcaggt cagcgattct cactgtttac cctgacagtc gatacccatc acccggatgg
     4681 ttttatctct cgtacctgta accgcaaaaa atatgatttt gacggtaaac cgaatcagtc
     4741 attcagcgcg gtaagttgca gccaggagaa tatcgcgacg tttatcaaca aaatcaaagc
     4801 gtcaccgtgg tttaaagata ctgtcatcgt cgtctcttct gaccatttag cgatgaacaa
     4861 cacggcgtgg aaatacctca ataagcagga tcgcaataac ctgttttttg tcattcgtgg
     4921 cgacaagccg cagcaagaga cgctggcagt gaagcgtaac acgatggata acggagcgac
     4981 agtgctggac attctcggtg gcgataacta tctcggactt ggtcgtagca gtttatccgg
     5041 gcagtcaatg tcggaaatct tcctcaatat caaagagaaa acattggcgt ggaagccgga
     5101 tatcatccgt ctgtggaaat tccctaaaga gatgaaagag ttcaccatcg accagcagaa
     5161 aaacatgatt gccttctcgg gtagccattt ccgtttgccg ctgctgttgc gggtttcaga
     5221 caaacgcgtg gaaccgctgc cagaaagcga atactcagca ccgctgcgtt tccagctggc
     5281 cgatttcgct ccacgcgaca atttcgtctg ggttgaccgt tgctacaaga tggcacaact
     5341 ctgggctccg gaactggcac tttccaccga ctggtgtgtc tcgcaggggc aacttggcgg
     5401 tcagcaaatt gttcagcatg ttgacaaaac aacatggaag agcaagacgg catttaaaga
     5461 tacggtcatc gacatggcgc gttacaaagg caatgtcgat acgctgaaga ttgttgataa
     5521 cgatattcgc tacaaagccg acagtttcat ctttaacgtc gccggtgcgc cagaagaggt
     5581 gaaacagttt agcgggattt cccgtccgga gtcgtggggc cgctggtcca acgcgcagct
     5641 gggcgatgaa gtaaaaatcg agtacaagca tccgctgccg aagaaatttg acctggtgat
     5701 taccgccaaa gcatacggca ataacgccag ccgtcctatt ccggtacgcg taggcaatga
     5761 agaacaaact cttgtgctgg gcaatgaagt gaccaccacc acactgcatt tcgataaccc
     5821 aaccgatgcc gacacactgg taattgtgcc gccggaacct gtctcaacca acgaagggaa
     5881 tatcctcgga cactcgccgc gtaagctcgg gatcggcatg gtggaaatta aagtggtaga
     5941 acgtgaaggg taattcctga ctgatgtgta ggtcgcatag ggtgcgacct acacaccttc
     6001 tccggcttac aacacattat taagaaacaa aaaaaacagc gcccaacact tccccggacg
     6061 ctgcatggca actttaataa tctcgccttt cagaaagtaa ttacgccttt aattaactca
     6121 cgattgttaa tcacatcgcg ctcgtaggtt tctgccagcg tggcgaacgg atagcgatgg
     6181 gttaacatca tgccagcagt gatttttcct tccgccatca gtcgaccgac tttagcaaaa
     6241 tcttccggcg tggcgttgcg gctgcccatc atcgtcgttt cttttttatg gaattccgga
     6301 tcggagaact gcaactcacc tttaaacagg ccgacaaata ccaccgtgcc gccgtgacga
     6361 atcagattca cggtgttatt catcgcatgt tgattacctg tcgcgtcgat cactttctgc
     6421 gccagcgaac caccaaactg cgcccgcagc tgcgcgtcaa aatcttcggc tgacgggtcc
     6481 agcacgggca actccagacg cgttgccaca tgttcacggc gcgccggact ggtatccgcc
     6541 accaccacct gtgcgccatc ggctttagcg attgccgccg cgcccagacc gattggcccg
     6601 gccccgacca ccagcacctg ctcgccggga gcaatggctg cgcgacgcac cgcatgagcg
     6661 ctaatagcga aaggttcaat caatgcggcc gcctgcgggt caataccgtc tgcgggcaaa
     6721 atgttcgcca ccggcacgct caaatactca ctaaaaccgc catcctgatg cacgccaatg
     6781 actgaaattt tttcacagca attggtacgc ccgcttttac acgccgggca ttgctgacag
     6841 gcaacataag ggatcacagc aacttgctga ccatttttaa gatcagcaat atttttaccc
     6901 agcccaacaa tctccccaca tatttcatgg cctaaaaccc gtggataact aaaaaatggt
     6961 tgatttccac cccaggcatg aatatcggta ccgcaaatcc cgacagactt aatttttatt
     7021 aatgcttcat tgtcacccgg aataggtatc tcgcgttgtt tccagactaa ttctttcggc
     7081 tgctggcaaa ttaaaacatt catcgtagac atgattcggc tctccatttt ttgttgcctt
     7141 agatatcaat gaaaatgaaa aatggcagcg gaacggaaaa tctgttttgt gaaaacacac
     7201 gcataaaaat gttttaaatc gggttttaat ggactcacaa ggaggaaaat atgagtcgtt
     7261 cacaaaattt acgccacaat gtgattaacc aggtcattga tgatatggcg cggggtcata
     7321 tcccttcccc acttccatcg caaagtgcgc tggcggaaat gtacaacatc agccgcacta
     7381 cagtgcgtca tattctcagc cacttacgcg aatgcggcgt cttaacgcag gttggaaacg
     7441 actatgttat tgcgcgtaaa cctgaccatg atgacggttt cgcctgtact accgcttcaa
     7501 tgagtgaaca aaacaaagtg tttgagcagg cttttttcac gatgataaac cagcgccagt
     7561 tacgcccagg ggaaactttt tctgaactgc aactggctcg ggccgcgggt gtcagcccgg
     7621 tggttgtaag agaatatctt ttaaaattcg gccgttataa tcttattcaa agcgaaaagc
     7681 gcggccagtg gagcatgaag caattcgatc aatcctacgc tgagcagctg tttgagctac
     7741 gcgaaatgct cgaaacacat tcacttcagc actttctcaa cctgcctgat catgatcccc
     7801 gctggctgca ggcgaaaacc atgctcgaac gtcatcggct actgcgtgac aacatcggca
     7861 atagctttcg catgttctcg caacttgaca gagattttca ctccttactg ctttcagctg
     7921 ccgacaatat cttttttgat caatcgcttg agatcatctc agtcatcttt cattttcact
     7981 atcaatggga cgaaagcgat cttaaacagc gcaatatcat tgccgtcgat gagcatatga
     8041 ccattctcag cgctctgatt tgccgcagtg accttgatgc cacccttgcg ctccgtaacc
     8101 atttgaattc agcgaaacaa tcaatgattc gatccattaa tgagaacacg cgttatgctc
     8161 attaagtacg gattaaaaac agcaaactat tagcgggtgc acggttttat caaaagataa
     8221 ataccgggaa attcccgctt acctatgctc acaatcagtc gactaaaaat gtttttgtga
     8281 gcggtagtaa agtcctaaaa ctttaacctg ccgttaatgt caaccttgcg taatgcacaa
     8341 ttacgtaaac aattaaaatt ataaatacag gagtccggcg tggaaaaaga aaatatcacc
     8401 ctcgatccgc gttcttcatt tactccatct tcgtcggcag atattcccgt gccaccagat
     8461 ggattagttc aacgcagtac ccgaattaaa cgcattcaaa ccaccgccat gttgttatta
     8521 ttttttgcgg cggtaatcaa ttatctcgac cgcagttcgc tgtcggtagc aaatttaacg
     8581 attcgtgaag aattgggatt aagtgccacc gaaatcggcg ctttgctctc cgtgttttca
     8641 ctcgcttacg gcattgcgca acttccttgc ggcccactat tggatcgtaa aggcccacgc
     8701 ctgatgctgg gactggggat gttcttctgg tcactgttcc aggccatgtc tggcatggtg
     8761 cacagcttta cgcagttcgt gttggtgcgt atcggtatgg ggattggtga agcgccgatg
     8821 aacccatgcg gtgtaaaagt cattaacgac tggttcaaca tcaaagagcg cggacgcccg
     8881 atgggcttct tcaacgcagc ttctaccatt ggcgttgccg taagcccacc gattctggcg
     8941 gcgatgatgc tggtgatggg ctggcgcggg atgtttatta ccattggtgt actggggatt
     9001 tttctcgcca tcggctggta tatgctctat cgcaaccgcg agcacgtaga actgactgcc
     9061 gttgaacaag cttatctcaa tgcaggtagc gtcaatgccc gccgagatcc gctcagtttt
     9121 gccgaatggc gcagcctgtt ccgtaaccgc acaatgtggg gaatgatgct cggattcagt
     9181 ggcatcaact acactgcgtg gctgtatctg gcctggcttc ctggttacct gcaaacagcc
     9241 tataacctgg atttaaaaag cacagggttg atggcggcta tccctttcct gtttggtgct
     9301 gccgggatgc tggtcaacgg ttacgttact gactggctgg tcaaaggggg aatggctccg
     9361 attaaaagcc gtaagatctg cattattgcc gggatgttct gttctgccgc ctttacgctg
     9421 atagtaccac aagcgacaac atccatgatg gcggttctgc tgattggcat ggcactgttc
     9481 tgtattcact ttgccggaac atcctgctgg ggcttgatcc acgtcgcagt tgcttctcgc
     9541 atgactgcgt cggtgggcag tatccagaac tttgccagct tcatctgcgc ctctttcgcg
     9601 ccgatcatta ctggttttat tgttgataca acccactcat tccgtctggc actaatcatt
     9661 tgcggttgcg tcaccgcagc gggagcactg gcgtacatct tcctggttcg tcagccgatc
     9721 aacgacccgc gtaaagatta aactccctat gcccgataac attttgctta tcgggcattt
     9781 ttatggcgat taaaacgttt cccagttctc cccgctatct gccactgcca ttttacgcgg
     9841 cgtagctggc gtcacggttt ttaccacagc cgatgtttca cgctgctgtt gctgctgttg
     9901 ctgaatccgg aacactgcca cagcttcggt caggcgactg gcctgctctt ccaacgcggc
     9961 ggcggcagcg gcagactctt ccaccagcgc agcgttctgt tgagttaccc ggtccatctc
    10021 agcaaccgct aagccaacct gatcgatacc acggctctgc tcatcagaag cagaagctat
    10081 ttcgcccata atgtccgtca cgcgggtcac agcactgaca atctccgcca ttgtttcccc
    10141 ggcgctttcg accagcgtag agccaacatc cactttcccc accgagtctt caatcaggct
    10201 tttaatttca cgagccgcct gggcgctacg ctgggccaga ttacgcactt ctcccgcaac
    10261 caccgcaaaa ccacgtcctt gctcacccgc acgcgctgct tcaaccgccg cgttcaaagc
    10321 cagaatattg gtctggaagg caatgccgtc aattacgctg ataatatcgg cgattttctg
    10381 cgaactggtg gagatatcac gcatagtctg caccacgtta tctaccactt tgccgccgcg
    10441 ttgcgccgtt tcagaagcac ttaacgccag gtggctggcc tggcgcgcat tctcggcgtt
    10501 ctgtttcacc gttgcggtga gttgctccat gctggctgcc gtctcttcca gcgaagcggc
    10561 ctgttgctcg gtgcgcgaag agagatcgtt attgccggta gcgatttcgc tggcaccgct
    10621 atagatggca ttggccccgt tgcgcacatc accgacggta cgcatcagct ctccctgcat
    10681 atggcgcaaa ctctctgcca gttgccccat ctcattagag ccatccacct caatcggttt
    10741 caccagatcg ccgcctgcaa tatgacgaat gctgtcaatc aggcgattca ttggcgctac
    10801 cagcgaggct ttaataccga accagacggc gaagatgacc gccagtacga cgatcatcac
    10861 gcccaccaga atccacatcg cctggctgta ggaggcattg ttatcgctga cggcgatatc
    10921 atagagccga tcgttttgct ccatgtaagc cacatactgc ttctcgaaac cgtcctgata
    10981 tccctgggtc ggctgatcaa agaactcgtt gattttgcct gcacctaaca gttggatcag
    11041 ctccgccagc gcattgtgat aaatatcgta attacgtttg atctctgccg ctgcggcggt
    11101 gctctgacgc gggtcacgcg gcaacgcttc gtaatctgcc cagttttttt ccgcctgttt
    11161 caacgaaata ctggcactct gcatcagctc agcaacggtt gaaccgctac caatattatt
    11221 ctgatccatc atgtagcgga tacccgcgcg gttgagggtg ttacgcgtct gcaacaacgc
    11281 gacccagctg ccattcagcg tggattgctg ctggcgaatg gtttgtaaaa cagtgaaatt
    11341 ttctttgtca ttctttaagg cattaaagaa cagaccgcct gatgtcagtt gtaaaaggcc
    11401 aaaaacggcc aaaaccagca gtaagctggt cacgatcttg atacgtttta acatggtttc
    11461 tctttcctgt ggaccgatgc aaaattttcg gcctgaaatg ag
//
LOCUS       DAZKIP010000128        11439 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZKIP010000128 DAZKIP010000000
VERSION     DAZKIP010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.129"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(107..1189)
                     /gene="lptG"
                     /locus_tag="ACHOUK_003864"
     CDS             complement(107..1189)
                     /gene="lptG"
                     /locus_tag="ACHOUK_003864"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFP9899216.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(1189..2289)
                     /gene="lptF"
                     /locus_tag="ACHOUK_003865"
     CDS             complement(1189..2289)
                     /gene="lptF"
                     /locus_tag="ACHOUK_003865"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFP9899217.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(2352..2402)
                     /gene="ytgA"
                     /locus_tag="ACHOUK_003866"
     CDS             complement(2352..2402)
                     /gene="ytgA"
                     /locus_tag="ACHOUK_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFP9899218.1"
                     /translation="MSIIILATISTTQENK"
     gene            2556..4067
                     /gene="pepA"
                     /locus_tag="ACHOUK_003867"
     CDS             2556..4067
                     /gene="pepA"
                     /locus_tag="ACHOUK_003867"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFP9899219.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            4421..4864
                     /gene="holC"
                     /locus_tag="ACHOUK_003868"
     CDS             4421..4864
                     /gene="holC"
                     /locus_tag="ACHOUK_003868"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFP9899220.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            4864..7719
                     /gene="valS"
                     /locus_tag="ACHOUK_003869"
     CDS             4864..7719
                     /gene="valS"
                     /locus_tag="ACHOUK_003869"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFP9899221.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(7774..8970)
                     /gene="yjgN"
                     /locus_tag="ACHOUK_003870"
     CDS             complement(7774..8970)
                     /gene="yjgN"
                     /locus_tag="ACHOUK_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFP9899222.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFFSCLVFVFFYFAILMTVSADMPLIGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGVWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIVSVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIM
                     PSLPFL"
     gene            9163..9666
                     /gene="yjgM"
                     /locus_tag="ACHOUK_003871"
     CDS             9163..9666
                     /gene="yjgM"
                     /locus_tag="ACHOUK_003871"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9899223.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALKAMEEAREMGFKRCYLETTAFLKEAIGLYEHLGFQHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(9712..10128)
                     /gene="rraB"
                     /locus_tag="ACHOUK_003872"
     CDS             complement(9712..10128)
                     /gene="rraB"
                     /locus_tag="ACHOUK_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFP9899224.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            10290..11294
                     /gene="argF"
                     /locus_tag="ACHOUK_003873"
     CDS             10290..11294
                     /gene="argF"
                     /locus_tag="ACHOUK_003873"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFP9899225.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKPFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
BASE COUNT         3018 a         2929 c         2893 g         2599 t
ORIGIN      
        1 accccactgc aatatattga attttaatta tttttcagga atgaacgaag cacactttgc
       61 taacaataat aaagggagct ttcgctccct ttattcgttc attcggttac gattttctca
      121 ttaacagcca caggctgatt aagaagaagc tggcgcttgg caacagtgcg ccgatgatcg
      181 gcgggatgcc ataaaccaac gtcagcgggc cgaagatctg gtccagtacg tagaagacaa
      241 aaccgaaact gataccggtg accacacgca cgcccatcgg tacgctacgc agtgggccaa
      301 agatgaacga cagcgccatc agcatcatca ccgccacgga tagcggctgg aagattttgc
      361 tccacatgtt gagctgataa cgtccggcat cctgaccgct cgacttcaga tacttcacat
      421 agttgtgcaa accgctgata gagagtgcat ccgggtccag cgccaccacg cccagtttgt
      481 ccggcgtgag gttggttttc caggtgccgc tcaccgtctg cgaaccggta atctgtttcg
      541 gattggtcag atcagattca tcaacctgcg acaaacgcca gactttatgt tccgggtcaa
      601 acttcgcagt agcggcatag cgtacggatt gcagacgacg attctcgtta aaggcataaa
      661 tgctgatgcc acctaactct tcgtcacctt taacccgctc aatgtagacg aagttgttgc
      721 catctttcgc ccataagcct tgctgggtag agagcaacga gccgccgtac atcgcctgcg
      781 cacggtagtt acgcgccatc tgctcgccct gcggcgcgac ccattcgcca atcgccatcg
      841 tcagcaagac cagcggaatg gcggttttca tcaccgacag cgccacctgc atacgggtaa
      901 aaccagaagc ctgcatcacc accagttcgc tgcgctgcgc cagcatccca agaccaagca
      961 acgccccaag cagagccgcc atcgggaaga atatttgcac atctttcggc acgctcagca
     1021 aggtatacat tcctgcgcct aacgcgtcgt aactcccctg cccggctttt ttcaactgat
     1081 cgacaaactt gataatgccc gacagcgaca ccagcatgaa cagcgtcatc atgatggtgg
     1141 tgaaaatagt tttaccgata tagcggtcaa gtacgccaaa aggttgcatc acaccgctcc
     1201 tttacgcgaa aaactggcgc gcaggcggcg gaccggcacg gtgtcccaaa ggttgagaac
     1261 aatcgccaaa gccagataaa tcaggttaac ggtccacatc cacagcgtcg ggtccagctt
     1321 acctttaccg ccgttcgatt tcagggaggt ctggatcagg aagaaaagta gatacagcag
     1381 catggctggc agcatcgaca gtacgcgtcc ctgacgtggg ttaaccacgc tcagcggtac
     1441 gaccataagt gccatcataa acacggtgaa taccaacgtg atacgccagt tcagttctgc
     1501 gcgagcacga tcggtgtcag tgttccacaa tgtgcgcatg tccatctggt cggtatcgtt
     1561 cgggtcgagc gccaccgcct ggtgaccaat gatcgcctga taatcctgga aatccgtaat
     1621 gcggaaatca cgtaacagtg cagtgccttc gaagcgcgtt ccctggttga gagtgacgac
     1681 ctgggagccg tcgcgcagct gggttaaatg tccggaatcg gccaccacca cagaaggacg
     1741 tgcattacct tttggtcgaa tttgcgcgag gaacacatct ttgaaatcgc tgccgtcaac
     1801 gctttcgatg aacagcaccg aactgccatt agtcgcttgc tggaattgcc cttgcgccag
     1861 cgccgccatg ccagggttcg ctttcgcttc tgctaacact tcatcctgat gacgcgatga
     1921 ccacggtccc gcccacatca cgttaaccgc cgcgacgatt gccgtgaata ccgcaaggat
     1981 cattgccgct ttcaccagaa ccgctttgct caggccgcag gcatgcatta ccgtaatttc
     2041 actttcggta tacagtttgc ccagcgtcat cagcagcccg aggaacaggc ttaatggcag
     2101 gataagctgc gccatttccg gcacgcccaa cccgagaagg gagagcacca gattcgccgg
     2161 aatatcgccg tcaaccgctg cgccgaggat cctcactaac ttttgacaga agaagatcaa
     2221 aagcaagatg aagagtatcg ccagctggct tttgagcgtc tcccgcacca gatatcttat
     2281 gattatcact ttaaatacgc ccgtaaaaac tcgtcttttg caggatttta gcttgtttca
     2341 tggcttaaac gtcatttatt ctcttgagtc gtcgaaatcg tcgctaagat aattatactc
     2401 aacggattca cctctcagat tttgttctga cgtgccaatg ccgtaataac gttaagatta
     2461 acacgaagtc atcgcaacag cggacatgag ttacgaaagc ttgcaattct atctgtagcc
     2521 accgccgttg tctttaagat tcaggagcgt agtgcatgga gtttagtgta aaaagcggta
     2581 gcccggagaa acagcggagt gcctgcatcg tcgtgggcgt cttcgaacca cgtcgccttt
     2641 ctccgattgc agaacagctc gataaaatca gcgatgggta catcagcgcc ctgctacgtc
     2701 ggggcgaact ggaaggaaaa ccggggcaga cattgttgct gcaccatgtt ccgaatgtac
     2761 tttccgagcg aattctcctt attggttgcg gcaaagaacg tgagctggat gagcgtcagt
     2821 acaagcaggt tattcagaaa accattaata cgctgaatga tactggctca atggaagcgg
     2881 tctgctttct gactgaactg cacgttaaag gccgtaacaa ctactggaaa gtgcgtcagg
     2941 ctgtcgagac ggcaaaagag acgctctaca gtttcgatca gctgaaaacg aacaagagcg
     3001 aaccgcgtcg tccgctgcgt aagatggtgt tcaacgtgcc gacccgccgt gaactgacca
     3061 gcggtgagcg cgcgatccag cacggtctgg cgattgccgc cgggattaaa gccgcgaaag
     3121 atctcggtaa tatgccgccg aatatctgta acgccgctta cctcgcttca caagcgcgcc
     3181 agctggctga cagctacagc aagaatgtta tcacccgcgt tatcggcgaa cagcagatga
     3241 aagagctggg gatgcattcc tatctggcgg tcggtcaggg ttcgcaaaac gaatcgctga
     3301 tgtcggtgat tgagtacaaa ggcaacgcgt cggaagatgc acgcccaatc gtgctggtgg
     3361 gtaaaggttt aaccttcgac tccggcggta tctcgatcaa gccttcagaa ggcatggatg
     3421 agatgaagta cgatatgtgc ggtgcggcag cggtttacgg cgtgatgcgg atggtcgcgg
     3481 agctacaact gccgattaac gttatcggcg tgttggcagg ctgcgaaaac atgcctggcg
     3541 gacgagccta tcgtccgggc gatgtgttaa ccaccatgtc cggtcaaacc gttgaagtgc
     3601 tgaataccga cgctgaaggc cgcctggtac tgtgcgacgt gttaacttac gttgagcgtt
     3661 ttgagccgga agcggtgatt gacgtggcga cgctgaccgg tgcctgcgtg atcgcgctgg
     3721 gtcatcacat taccggtctg atggcgaacc ataatccgct ggcccatgaa ctgattgccg
     3781 cgtctgaaca atccggtgac cgcgcatggc gcttaccgct gggtgacgag tatcaggaac
     3841 aactggagtc caattttgcc gatatggcga acattggcgg tcgtcctggt ggggcgatta
     3901 ccgcaggttg cttcctgtca cgctttaccc gtaagtacaa ctgggcgcac ctggatattg
     3961 caggaaccgc ctggcgttct ggtaaagcaa aaggcgcaac cggtcgtccg gtagcgttgc
     4021 tggcacagtt cctgttgaac cgcgctgggt ttaacggcga agagtaattg cgtcaggcaa
     4081 ggctgttatt gccggatgcg gcgtgaacgc cttatccgac ctacacagca ctgaactcgt
     4141 aggcctgata agacgcgcaa gcgtcgcatc aggcaatgct gttattgtcg gatgcggcat
     4201 gaacgcctta tccgacctac acagcaccga actcgtaggc ctgataagac gcggcagcgt
     4261 cgcatcaggc aatgctgtta ttgtcggatg cggcgtgagc gccttatccg acctacacac
     4321 agcactgaac tcgtaggcct gataagacac aacagcgtcg catcaggcgc tgcggtgtat
     4381 acctgatgcg tatttaaatc caccacaaga agccccatat atgaaaaacg cgacgttcta
     4441 ccttctggac aatgacacca ccgtcgatgg cttaagcgcc gttgagcaac tggtgtgtga
     4501 aattgccgca gaacgttggc gcagcggtaa gcgcgtgctc atcgcctgtg aagatgaaaa
     4561 gcaggcttac cggctggatg aagccctgtg ggcgcgtccg gcagaaagct ttgttccgca
     4621 taatttagcg ggagaaggac cgcgcggcgg tgcgccggtg gagatcgcct ggccgcaaaa
     4681 acgcagcagc agcccgcgcg atatattgat tagtctgcga acaagctttg cagattttgc
     4741 caccgctttc acagaagtgg tagacttcgt tccttatgaa gattctctga aacaactggc
     4801 gcgcgaacgc tataaagcct accgcgtggc tggtttcaac ctgaatacgg caacctggaa
     4861 ataatggaaa agacatataa cccacaagat atcgaacagc cgctttacga gcactgggaa
     4921 aagcagggct actttaagcc taatggcgat gaaagccagg aaagtttctg catcatgatc
     4981 ccgccgccga acgtcaccgg cagtttgcat atgggtcacg ccttccagca aaccatcatg
     5041 gataccatga tccgctatca gcgcatgcag ggcaaaaaca ccctgtggca ggtcggtact
     5101 gaccacgccg ggatcgctac ccagatggtc gttgagcgca agattgccgc agaagaaggt
     5161 aaaacccgtc acgactacgg ccgcgaagct ttcatcgaca aaatctggga atggaaagcg
     5221 gaatccggcg gcacgattac ccgtcagatg cgccgtctcg gcaactccgt cgactgggaa
     5281 cgtgaacgct tcacgatgga cgaaggcctg tccaatgcgg tgaaagaagt tttcgttcgt
     5341 ctatataaag aagacctgat ttaccgtggc aaacgcctgg taaactggga cccgaaactg
     5401 cgcaccgcta tctctgacct ggaagtggaa aaccgcgaat cgaaaggttc gatgtggcac
     5461 atccgctatc cgctggctga cggtgcgaaa accgcagacg gtaaagatta tctggtggtc
     5521 gcgactaccc gtccggaaac cctgctgggc gatactggcg tagccgttaa cccggaagat
     5581 ccgcgttaca aagatctgat tggcaaatat gtcattctgc cgctggttaa ccgtcgtatt
     5641 ccgatcgttg gcgacgaaca cgccgacatg gaaaaaggca ccggctgcgt gaaaatcact
     5701 ccggcgcacg actttaacga ctatgaagtg ggtaaacgtc acgcgctgcc gatgatcaac
     5761 atcctgacct ttgacggcga tatccgtgaa agcgcccagg tgttcgatac caaaggtaac
     5821 gaatctgacg tttattccag cgaaatcccg gcagagttcc agaaactcga gcgttttgct
     5881 gcacgtaaag cggtcgttgc tgcggttgac gcactcggcc tgctggaaga aattaaaccg
     5941 cacgacctga cagttcctta cggcgaccgt ggcggcgtag ttatcgaacc aatgctgacc
     6001 gaccagtggt acgtacgtgc cgatgtactg gcgaagccgg cagttgaagc ggttgagaac
     6061 ggcgacattc agttcgtacc gaagcagtac gaaaacatgt acttctcctg gatgcgcgat
     6121 attcaggact ggtgtatctc tcgtcagctg tggtggggtc accgtatccc ggcatggtac
     6181 gacgaagcgg gtaacgttta tgttggccgc aacgaagaag aagtgcgcaa ggaaaataac
     6241 ctcggtgccg acgttgccct gcgtcaggac gaagacgttc tcgacacctg gttctcttct
     6301 gcgctgtgga ccttctctac cctaggctgg ccggaaaaca ccgacgcact gcgccagttc
     6361 cacccaacca gcgtgatggt atctggtttc gacatcatct tcttctggat tgcccgcatg
     6421 atcatgatga ccatgcactt catcaaagat gaaaatggca aaccgcaggt gccgttccac
     6481 accgtttaca tgaccggtct gattcgtgac gacgaaggcc agaagatgtc caaatccaag
     6541 ggtaacgtta tcgacccgct ggatatggtt gacggtattt cgctgccaga actgctggaa
     6601 aaacgtaccg gcaatatgat gcagccgcag ctggcggaca aaatccgtaa gcgcaccgag
     6661 aagcagttcc cgaacggtat tgagccgcac ggcaccgatg ccctgcgctt caccctggcg
     6721 gcactggcgt ctaccggtcg tgacatcaac tgggatatga agcgtctgga aggctaccgt
     6781 aacttctgta acaagctgtg gaacgccagc cgctttgtgc tgatgaacac ggaaggtcag
     6841 gattgcggct tcaacggcgg cgaaatgacg ctgtcgctgg cggaccgctg gattctggcg
     6901 gagttcaacc agaccatcaa agcgtaccgc gaagcgctgg acagcttccg cttcgatatc
     6961 gccgcaggca ttctgtatga gtttacctgg aaccagttct gtgactggta tctcgagctg
     7021 accaagccgg taatgaacgg tggcaccgaa gcagaactgc gcggtactcg ccatacgctg
     7081 gtgactgtac tggaaggtct gctgcgcctc gcgcatccga tcattccgtt catcaccgaa
     7141 accatctggc agcgtgtgaa agtactttgc ggtatcactg ccgacaccat catgctgcag
     7201 ccgttcccgc agtacgatgc atctcaggtt gatgaagccg cactggccga caccgaatgg
     7261 ctgaaacagg cgatcgttgc ggtacgtaac atccgtgcag aaatgaacat cgcgccgggc
     7321 aaaccgctgg agctgctgct gcgtggttgc agcgcggatg cagaacgtcg cgtaaatgaa
     7381 aaccgtggct tcctgcaaac cctggcgcgt ctggaaagca tcaccgtgct gcctgccgat
     7441 gacaaaggtc cggtttccgt tacgaagatc atcgacggcg cagagctgct gatcccgatg
     7501 gctggcctca tcaacaaaga agatgagctg gcgcgtctgg cgaaagaagt ggcgaagatc
     7561 gaaggcgaaa tcagccgtat cgagaacaag ctggcgaatg aaggctttgt cgcccgcgca
     7621 ccggaagcgg tcatcgcgaa agagcgtgag aagctggaag gctatgcgga agcgaaagcg
     7681 aagctgattg aacagcaggc tgttatcgcc gcgctgtaat caaaacacag tgatgaaaac
     7741 gaaggccgga gcatgctccg gcctttttat ctcttacaga aatggtaaag aaggcataat
     7801 tccccgtgaa atactggcta agaagccttt atctggttgt tcttctttat tgattaaagg
     7861 aaggtcatcc aaatcgccca gcaaatacgt attttttgcc tgccagtcaa tcatccatat
     7921 tttcagcagt ggataagcca gaccgcccgt aatcccggat atcaccacca acgcacacat
     7981 gcgataaagc ataccgtggt atgttaaagt tgagcgaaaa cgaatacgcc catcattcag
     8041 tgacaggttg ctcataaaat gatttcgcaa cgacaccgtt aaataactgg tgctaacagc
     8101 aatcccaaaa taatagatta actgcgcgat tatcatttta cgttgcattt ccataaattg
     8161 ctgtaaattc tcaatatcat catttgcata cacagaacta tcatacgcat ttaatatttg
     8221 atcgaagata atataaccag caacagcgag aaaaggtaat aaagctaaaa atgcgaggat
     8281 ggcatatttt atacaatacg cagtatctaa tttcaccttg aaacgatgta taccgaaact
     8341 ggtattgctc cagagaaaac tcattaccag gctatataaa gtaccattaa aaataccaat
     8401 ggaaacaata ccaactattg ccatcagaac cacagataca ataacactac ttgaactatt
     8461 ggcatgtagc atctttgtcg agataaagaa aacagtcccc atcccaatgg ccattaaaat
     8521 cggcaagaaa aaggttaccc accagacccc tttcatagag caattaaaac taaatcttac
     8581 gccgttgaga ctggtcatca aggcctgata acgcagtcct tttgctgcca taaatataag
     8641 caaaaccaac agtgacaaag tcaaaacaca gccaataagc ggcatatctg ctgacactgt
     8701 cattaagatt gcgaaataga aaaaaacaaa aacaagacaa ctaaaaaaaa cattcccacc
     8761 agtaattcca taagaaaatc gttgtccgtt aacttccata ttagcataga gataacgctt
     8821 acatttcatt aatgcccatg gtaaatagat acctagcgtt ataatcgtta acaacacatt
     8881 caccacacaa ataagaaaat atctctcacc tgtaccatga aaaacaaacg aatgggacgg
     8941 aacatccatt tcattaataa cttgagccat aaaaattccc tgaattaaaa aatccattga
     9001 tataatttat agatacagac agtaaaatgt tctgccacgt tacaatactc ttatcagtaa
     9061 taggctggca aaaactttat tgctttataa gacgcttgcg cacgtaagaa gagagtggta
     9121 ttaaacaaat gtataattac tttattttta ttgggttaaa ctatgaataa cattgcgccg
     9181 caatcacctg taatgcgtcg cctgacgctg caggataatc ctgctatcgc ccgcgtcatt
     9241 cgtcaggtat ccgccgaata cggtcttacc gctgataaag gctacaccgt cgccgatccg
     9301 aatcttgacg agctgtatca agtgtatagt cagccgggtc atgcatattg ggtggttgag
     9361 tacgagggtg aagtggtcgg cggcggtggg attgcgccat tagcaggtag tgaatcggac
     9421 atttgcgaac tgcaaaagat gtattttctt ccggctattc gcggcaaagg gctggcaaaa
     9481 aaactggcct taaaggcgat ggaggaggca agagaaatgg gcttcaaacg ctgctatctg
     9541 gaaacgaccg cttttttgaa agaagcgatt ggtttgtatg aacatttagg ttttcaacat
     9601 atcgactatg cacttggctg cacgggccat gttgattgtg aagtgcggat gctacgcgaa
     9661 ctctaaaaaa atgcccgcta gtgtgtgcta tcgggcattt taatcaatta attagtggcg
     9721 aaccccgtca tcgtcttcat cgacaaaatc ttcatcgtcg ccgtcttcgc cgttgggatc
     9781 ttcaaagtaa gtgccccaac cgtcgtactc aacgtcaaat ttctccgcca gcgtcatcag
     9841 ttgttcaacc tgggcatcga tcagatcggc attcaacgcg cactcgctga ggatgtcgca
     9901 gcaaatcacg atatcaccat cttcaacttc cagctcttct ggatcggtca cttcgtaacc
     9961 gagtttaaac gcttcaactg ctgctttttc cagggtttcc agatcgtctg cggaaagatg
    10021 gtgttcgatg gtgtacagcg cgtccggatc gctgccatct tccagtaatt cttcaataat
    10081 caaacgtgtt tcttcacgct gttcttccag ttgttccggg tttgccatgg ctcattcctc
    10141 ttaatgtcct gccgatactc atattgtcac acaccactgg cattgcctcc acctttgtga
    10201 caaagattta tgctttagac ttgcaaatga ataatcatcc atataaattg aattttaatt
    10261 cattgagacg ttagccacag gagggatcta tgtccgggtt ttatcataag catttcctga
    10321 aattactcga tttcacgcca gctgaactca acagcctgct gcagttagcc gcgaagctga
    10381 aagccgataa gaaaagcggc aaagaagaag ccaaactcac tggtaaaaac atcgcgctca
    10441 tcttcgaaaa agactcgacc cgtacccgat gctctttcga agttgccgca tatgaccagg
    10501 gtgctcgcgt tacttatctc ggcccaagcg gcagtcagat tggtcataaa gagtcgatta
    10561 aagacactgc ccgcgtgctg ggtcgcatgt atgacggtat tcagtatcgc ggctatggtc
    10621 aggagattgt cgaaacactg gcggaatacg ctggcgtgcc ggtatggaac ggcctgacca
    10681 atgagtttca ccccacgcag ttgctggcgg atctcctcac catgcaggag catttacccg
    10741 gtaaaccatt caacgaaatg acgctggtct atgcaggtga cgcacgtaac aacatgggca
    10801 attcgatgct cgaagctgcg gcgcttaccg gtctggattt gcgtctggtc gcgccacagg
    10861 catgctggcc ggaagctgcg ctggttacgg aatgccgcgc cctggcacaa caaaatggcg
    10921 ggaatattac gctgacggaa gatgtagcta aaggcgttga aggtgctgac tttatctata
    10981 ccgatgtatg ggtgtcgatg ggtgaagcaa aagaaaaatg ggctgaacgc attgcattac
    11041 tgcgtgatta tcaggtgaac agcaagatga tgcagttgac cggtaacccg gaggtcaaat
    11101 tcctccactg cctgcccgcg ttccatgatg accaaacgac gcttggcaag aaaatggcgg
    11161 aagagtttgg cctacatggc ggaatggaag tgactgatga ggtcttcgaa tctgccgcca
    11221 gcattgtttt tgatcaggcg gaaaaccgca tgcacaccat caaagcggtg atggtcgcga
    11281 cactcagtaa ataattgata tcgctataaa gatgccggga aatacagaaa catcccggcg
    11341 tcaaattatt ttatgcagtg atagtttttt tcttccagcc aaatttttct attgctatac
    11401 gtaaattttc cggaggattt agtgtattta aacgctgtt
//
LOCUS       DAZKIP010000129        11412 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZKIP010000129 DAZKIP010000000
VERSION     DAZKIP010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.130"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(250..2079)
                     /gene="glmS"
                     /locus_tag="ACHOUK_003874"
     CDS             complement(250..2079)
                     /gene="glmS"
                     /locus_tag="ACHOUK_003874"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFP9899226.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFAEQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2241..3611)
                     /gene="glmU"
                     /locus_tag="ACHOUK_003875"
     CDS             complement(2241..3611)
                     /gene="glmU"
                     /locus_tag="ACHOUK_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFP9899227.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3963..4382)
                     /locus_tag="ACHOUK_003876"
     CDS             complement(3963..4382)
                     /locus_tag="ACHOUK_003876"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFP9899228.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4403..5785)
                     /gene="atpD"
                     /locus_tag="ACHOUK_003877"
     CDS             complement(4403..5785)
                     /gene="atpD"
                     /locus_tag="ACHOUK_003877"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFP9899229.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5812..6675)
                     /gene="atpG"
                     /locus_tag="ACHOUK_003878"
     CDS             complement(5812..6675)
                     /gene="atpG"
                     /locus_tag="ACHOUK_003878"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFP9899230.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6726..8267)
                     /gene="atpA"
                     /locus_tag="ACHOUK_003879"
     CDS             complement(6726..8267)
                     /gene="atpA"
                     /locus_tag="ACHOUK_003879"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFP9899231.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8280..8813)
                     /gene="atpH"
                     /locus_tag="ACHOUK_003880"
     CDS             complement(8280..8813)
                     /gene="atpH"
                     /locus_tag="ACHOUK_003880"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFP9899232.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8828..9298)
                     /gene="atpF"
                     /locus_tag="ACHOUK_003881"
     CDS             complement(8828..9298)
                     /gene="atpF"
                     /locus_tag="ACHOUK_003881"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFP9899233.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9360..9599)
                     /gene="atpE"
                     /locus_tag="ACHOUK_003882"
     CDS             complement(9360..9599)
                     /gene="atpE"
                     /locus_tag="ACHOUK_003882"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFP9899234.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9646..10461)
                     /gene="atpB"
                     /locus_tag="ACHOUK_003883"
     CDS             complement(9646..10461)
                     /gene="atpB"
                     /locus_tag="ACHOUK_003883"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFP9899235.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10470..10850)
                     /gene="atpI"
                     /locus_tag="ACHOUK_003884"
     CDS             complement(10470..10850)
                     /gene="atpI"
                     /locus_tag="ACHOUK_003884"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFP9899236.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         2766 a         3074 c         2801 g         2771 t
ORIGIN      
        1 acatattgat gattgtttta caagcgattc ttgaatgaac aaaacgtgct attcattaca
       61 aagtacatca attatggcaa ttgtttgtca atgattcgag gtatttaatt actactacgg
      121 ttaaaacaag tgttcaacgg tgccccaaat aatagctgaa gttaattata tggaatgagg
      181 ttagattatt aatgctggta agtaaaaggg ccgccaaaag gcagcccttc gttcagcaaa
      241 tgaataatat tactcaaccg ttaccgattt tgccaggtta cgcggctggt caacgtcggt
      301 gcctttgatc agcgcgacgt gataagccag taactgcagc ggaacggtgt agaagatcgg
      361 tgcaatcacc tcttccacat gcggcatctc gatgatgtgc atgttatcgc tacttacaaa
      421 acccgcatcc tgctcggcga agacatacaa ttggccgcca cgcgcgcgaa cttcttcaat
      481 gttggatttc agtttttcca gcaattcgtt gttcggcgca acgacgataa ccggcatatc
      541 ggcgtcaatc agcgccagcg gaccgtgttt cagttcgcct gcagcgtagg cttcagcgtg
      601 aatataagag atctctttca gcttcaatgc gccttccagc gcgattgggt actgatcgcc
      661 acggcccagg aacagcgcgt gatgtttgtc agagaaatct tctgccagag cttcaatgcg
      721 tttgtcctga gacagcatct gctcaatacg gctcggcagc gcctgcagac catgcacaat
      781 gtcatgttca atggaggcat ccagaccttt caggcgagac agcttcgcca ccagcatcaa
      841 cagcacagtt aactgagtgg tgaatgcttt agtggatgcc acgccgattt ccgtacccgc
      901 gttggtcatc agcgccagat cggattcgcg caccagagaa gaacccggaa cgttacagat
      961 tgccagcgaa ccaaggtaac ccagctcttt cgacagacgc aggcccgcca gggtatccgc
     1021 ggtttcgcca gactgtgaca aggtgatcat caggctgtta cgacgcacgg cagatttacg
     1081 atagcggaat tcagaggcga tttcgacgtc gcacggaata cctgccagcg attcaaacca
     1141 gtaacgggaa accataccgg agttataaga agtaccacag gcgaggatct gaatatgctc
     1201 aaccttcgac agcagttcgt cggcgttcgg tcccagctcg cttaaatcaa cctgaccgtg
     1261 gctgatgcgc ccggtaaggg tgtttttgat cgcgttcggc tgttcgtaga tctctttctg
     1321 catgtagtga cggtaaatac ctttatcgcc cgcgtcatat tgcagattgg attcgatatc
     1381 ctgacgtttt acttccgcgc cagttttatc gaagatgttt accgaacggc gagtgatttc
     1441 cgcaatatcg ccctcttcaa ggaagataaa gcgacgggtc accggcagca gcgccagctg
     1501 gtcagaagcg ataaagtttt cacccatacc cagaccaatc accagcggac taccagaacg
     1561 tgctgccagc agggtatccg ggtgacggga gtccatgatc actgtaccgt acgcaccacg
     1621 cagttgcggg atagcacgca gaacagcctc acgcagagta ccgccctgtt tcagctccca
     1681 gttcaccaga tgggcaatca cttcggtgtc ggtttcagaa acgaaggtat agccacgcgc
     1741 ttttaactct tcgcgcagcg gttcatggtt ttcgatgatg ccgttatgca ccaccacaat
     1801 gtgttcagaa acatgcggat gcgcattcac ttctgaaggt tcaccgtggg tcgcccagcg
     1861 agtgtgagca ataccggtgc cgccatgcag aggatgttct tccgctgcct gagccagcat
     1921 ctggacttta ccgaggcgac gcaggcgggt catatgacct tccgcatcaa caacggccag
     1981 accggcagag tcatatccgc ggtattccag acgacgtaaa ccttcaagaa ggatttctgc
     2041 tacatcacgt tgcgcgatcg cgccaacaat tccacacata gtttttgatt ccgatttata
     2101 tcgttgtcgg tcaacctgta tgcccgtttt ttcgggcgcc ccgagccttg tagagagtgg
     2161 ggttattttt atagttactg cttgtgggag ggagattatg ttatctcctc atcccatgtg
     2221 accggattac ccggccagaa tcactttttc tttaccggac gacgccagcc ttctttctga
     2281 gtctgcggca cacggctgat agctaatgca ttttcgccga cattacgcgt cacagttgta
     2341 cccgcagcaa tggtcgcgcc tttgcctact gttaccgggg ccaccagctg agtgtcggaa
     2401 ccgacaaaca catcgtcgcc gataatggtc ttaaatttat tcgcaccatc gtagttgcag
     2461 gtaatggttc ccgcgccgat gttaacgtta tcgccaattt ccgcatcgcc aaggtaagtc
     2521 agatgaccag ctttcgagcc tttacccaga cgtgcttttt tcatctcaac aaagttaccg
     2581 acgtgtgcac cttccaacaa ctcagcacca ggacgcaaac gggcaaacgg gccaatggta
     2641 caggccgccg ccagattcgc gtcttccacg acggtatatg ggctaatttc gcaatcatcg
     2701 ccaatcacgc tgtttttaat cacgcaaccg gtgccgattt tcacgcgatg gccgagagtc
     2761 acgttgccct cgatgataac gttagtatca atttcaacat cgcgcccgtg agtaagcgta
     2821 ccgcgcagat caaaacgcgc cggatcgcgc agcataacgc ctgctaacag cagtttttca
     2881 gcctgttcgg actggtaaac gcgctccaga cgggagagtt gcaggcggtt attcacgcct
     2941 tctacttcgc ttaaacgttg cggatgaacg gcgacgattt cacgcccttc ctgatacgcc
     3001 agcgcaataa tgtcggtgat gtagtattcg ccctgcgcat tattgttggt cagcttcgcc
     3061 agccagcgtt tcatatctgc gccgttagcg atcagaatgc cggtgttgat ctcctgaatc
     3121 tgacgctgct cgtcggtggc atctttgtgc tcaacaatgc cggtaacttt gccgttttca
     3181 cgggtgatac gtccataacc ggtcggatca tccagtttca ccgtcagcag accaatgcca
     3241 ccctgcggtt tagcatcacg cagacgctgg agtgtttcga cagagatcag cggcacgtcg
     3301 ccgtagagca ttaaaatgtc ttcatcatcg gcaaagaaag gtgcggcctg ctgcatggca
     3361 tgacccgtac ccagctgctc tgcctgaagc acccagttca ggttgtcgtc tttcagcgcc
     3421 tgttttagca gatcgccacc gtgaccgtac accaggtgaa cgtgcgctgc gcctaattca
     3481 ttcgcagcat caatgacatg ctgaaccatc gctttcccgg caagggtatg cagcactttc
     3541 ggaagatcgg aatacatgcg cgtgccttta cctgcggcaa ggatcactac gctcatagca
     3601 ttattcaaca tacgcgtcct gactgtaatt tgagaacgaa tttaaaccgc ttcaccttga
     3661 aaaaactaca tttttttcat cgtgaaatgg acagaggata aattgttcaa taacggatta
     3721 tccccgctga cgaaagcgcc attttcgacc atcgtcacgc cttttgtctg cagaaaatta
     3781 aggcagtacc ttcatttaag cgtcaggtgg atgtttttgc tcttatttcg atcaatgaat
     3841 aaacgggaaa acagggggtt ttatatcagc aggatctatg tgaacgctat tcaggacggg
     3901 tcacacgcgc aaaaaaaagc cagcctgttt ccagactggc ttttgtgctt ttcaagccgg
     3961 tgttacatcg cttttttggt caactcgata acgcgcagct gcgcgatcgc tttggccagt
     4021 tccgcagacg cctgagcgta atctacgtcg ccgtgagagc tgctaatgtg ctcttcagcc
     4081 ttacgtttcg cttccatggc tcgcgcttcg tcgagatcct ggccgcgaat tgcggtgtcg
     4141 gccagaacgg tcacgttgcc aggctgcact tcaagaatgc cgccagacag atagataaac
     4201 tcttcgtgac cgtgctgttt cacgatgcga atcataccag gcttaatggc ggtgagcagc
     4261 ggtgcgtggc cagggtagat ccccagttca ccttcgctac ccgttacctg gattttctcg
     4321 accagaccag agaacatttg ttgctctgcg ctgacgacgt ccaggtggta agtcattgcc
     4381 atatcaccct ccgattaagg cgttaaagtt ttttggcttt ttccacagct tcttcgatgg
     4441 aaccgaccat gtagaacgcc tgctccggca ggtgatcgta ttcgccttcc atgatgcctt
     4501 taaagccacg gatggtgtct ttcagggaga cgtatttacc cggagaaccg gtgaatactt
     4561 ctgccacgaa gaacggctgg gacaggaagc gctggatctt acgagcacgc gctaccacca
     4621 gtttgtcttc ttcagacagt tcatccatac ccaggatggc gatgatgtct ttcagttcct
     4681 gataacgttg caggatggac tgaacgccac gcgcggtgtc gtagtgttcc tgaccaacca
     4741 ccagcgggtc cagctgacgg ctggtggagt ccagcgggtc aacggccggg tagataccca
     4801 gagacgcgat ctgacggctc agtaccacgg ttgcgtcaag gtgcgcaaag gtggttgccg
     4861 gagacgggtc agtcaagtca tccgcaggta cgtatactgc ctgtacggag gtgatagaac
     4921 cggttttggt ggaggtgata cgttcctgca gaacgcccat ctcttccgcc agggtcggct
     4981 gataacctac cgctgaaggc atacggccca gcagtgcgga tacttccgta ccggccaggg
     5041 tgtaacgata gatgttgtca acgaacagca gaacgtcacg accttcgtca cggaatttct
     5101 cagccatggt cagaccggtc agagctacgc gcagacggtt tcccggcggc tcgttcatct
     5161 ggccatacac cagggatact ttatcgataa cgttggagtc ggtcatttcg tggtagaagt
     5221 cgttcccctc acgagtacgt tcacctacgc ccgcaaacac agagtaaccg gagtgctcga
     5281 tcgcgatgtt acgaatgagc tccatcatgt ttacggtttt acctacaccc gcaccaccga
     5341 acagaccaac tttaccgccc ttagcgaacg gacacatcag gtcgataact ttgataccgg
     5401 tttccagcag ttcctgagag tttgacagct cttcgtagga aggtgctgcg cggtgaatcg
     5461 cccaacgctc ttcttcaccg atctcgcctt tcatgtcgac cggttcaccc agtacgttca
     5521 tgatacggcc cagagtcgct ttacctaccg ggacttcgat cgggtgttcg aggtctttta
     5581 catccagacc gcgacgcaga ccgtcggagg aacccattgc gatggtacgc acgataccgc
     5641 cgccgagctg ctgctgaact tccagcacca gacgctcatt accattttgc acctcaagag
     5701 catcgtacac gcgcggtacg gcatcctgag ggaattcgac gtcaactacg gcgccgatta
     5761 cctggacaat ctttccagta gccatcttaa atcctctacg aaataacctg tttaaaccgc
     5821 ggcggccccc gagacgatct cggtgagttc ctgagtaatg ctggcctgac gagctttgtt
     5881 gtataccaac tgcagctctt taatcaggct gccgccattg tcggtcgcgg ctttcatcgc
     5941 caccatacgg gcggcctgct cgctggccag gttttcaacc acgccctgat aaacctgaga
     6001 ttcgacataa cgacgcagca gggtatccag caacgccttc ggatcgggtt cgtacaggta
     6061 atcccaggat ttatgtttca gatcatcatc atctgatgcc ggtaacggca gcagctggct
     6121 gatggtcgga acctgagaca tggtgttaat aaatttgttg ctgacaatgt aaagcttgtc
     6181 cagacggcct tcgtcgtagg cctgcaacat cacttttacc ggaccgatca gttcggacag
     6241 ggaagggtta tcccccatgc cggtgacctg ggcaacaaca ttgccgccca cggagttgaa
     6301 gaacgacacg cctttcgagc cgatcattgc gaggtcgcat tgaacgcctt tgtcggtcca
     6361 ggtcttcatt tccgccagca gttttttgaa caggttaatg ttcaaaccac cgcacaaacc
     6421 acggtcggtc gacaccacca ggtagcccac gcgtttaacg tcgcggtctt ccaggtaagg
     6481 gtgcttatat tccagattac cgtgtgcaag gtgaccaatc actttgcgca tggtttctgc
     6541 ataaggacgg ctggccgcca tgcgatcctg cgatttacgc attttggaag cggcgaccat
     6601 ctccatcgct ttagtgatct tttgcgtgtt ctggacgctt gcgatcttac tacgtatctc
     6661 ttttgcgccg gccatgagct tctcctcaat gccttgcggc ctgccctaag gcaagccgcc
     6721 agacgttacc aggattgggt tgctttgaag gaatcgagga tgcctttcag cttgccttcg
     6781 atttcgtcgt tgtagccacc ggtctggttg atctcttgca tcaacggagc gtgatcacgg
     6841 tcgacgtaag ccagcagagc ggcttcgaag ctgccgattt tcgacagttc aacatccgcc
     6901 aggtaaccac gttctgctgc gaacagaacc agagactgct gcgcaacgga catcggcgca
     6961 tactgtttct gtttcagcag ttcggtcact ttttgaccgt ggtcaagctg cttacgtgtt
     7021 gcatcgtcaa ggtcggatgc aaactgagag aacgctgcca gttcacgata ctgtgccaga
     7081 gcggtacgga taccaccgga cagttttttc atgatcttgg tctgtgctgc accaccaaca
     7141 cgggatacgg aaatacccgg gttaaccgca ggacgaatac cggcgttgaa caggttggtt
     7201 tccaggaaga tctgaccatc ggtaatggag attacgttgg tcggaacgaa cgcagaaacg
     7261 tcacccgctt gagtttcgat aatcggcagc gcggtcagtg aaccggtttt ccctttcact
     7321 tcacctttgg tgaaagcttc aacgtattcg gcgttaacac gcgcagcacg ctccagcaga
     7381 cgagagtgga ggtagaatac gtcgcccggg aatgcttcac gtcctggcgg acgacggagc
     7441 agcagcgaga tctgacggta agcaacagcc tgtttagaca gatcatcgta aatgatcagc
     7501 gcatcttcac cgcggtcacg gaagtattcg cccattgcgc aaccggcata cggtgccagg
     7561 tattgcagtg cagcggattc agacgcggtt gctaccacaa cgatggtgtt agccagtgcg
     7621 ccgtgctctt ccagtttacg taccacgtta gaaatggtgg acgctttctg gccgatagcg
     7681 acatagatac atttgatacc ggaatcgcgc tggttgatga tggcatcgat agccagtgcg
     7741 gttttaccgg tctgacggtc accgatgatc aattcacgct gaccacgacc gattgggatc
     7801 atggagtcaa cggctttata accggtctgt accggctgat ctacggactg acgttcgata
     7861 acgcccggag cgattgcttc tacagcagag aagccgtcgt gatccagcgg acctttaccg
     7921 tcgattggtg cacccagagt gttaaccaca cggcccagca ggccacggcc aaccggaact
     7981 tccaggatac ggccagtaca cttaactttc atgccttcgg caaggtcagc atacggaccc
     8041 ataacaaccg caccaacaga gtcgcgctcg aggttcagtg cgatagcgta acggttaccc
     8101 ggcagggaga tcatttcacc ctgcatacaa tcggccaggc cgtgaatgcg gataacaccg
     8161 tcacttacag aaacaatagt accttcgttg tgagcttcac tcacaacatt gaactgagca
     8221 atgcgctgct tgatcagttc gctgatttcg gtggaattca gttgcatgct ccagtcccct
     8281 taagactgca agacgtctgc aaggcgctca agacgaccgc gtacgctgcc atcaatgacc
     8341 atatcacccg ctcggatgat aacgcctgcc attacagact tatcgatttt gcaattcagc
     8401 ttaactttgc gtgacagacg tttttccatc gcagcagaaa ttttcgcgag ctgttgttca
     8461 ctcagtgcgg cagcggaaat gacgtctacc tcagcggtag cctcactcac ggcacgaagg
     8521 tgaataaact gctccaaaac atccgggagc gcgttaagac gaccattttc agccattacc
     8581 cgaataaggt tctgaccgtt ttcgtccagt tgctcaccac aaactgcgat aaacgactcg
     8641 gcaagcgttt ctggcgcaag cgcgccagag agaagctctg ccatttgttc gtttttggtt
     8701 acctcggcgg caaacgccag catgtcctgc cagcgttcta cactttggtg ttcgacggca
     8761 aagtcaaaag ctgctttggc gtaggggcga gctaccgtaa taaattcaga catcagcccc
     8821 tccctcctta cagttcagcg acaagtttat ccacgatgtc gctgttagca gcttcatcca
     8881 cggaacgttc gatgatcttc tcggcgccag caacagccag gatagcaact tgcttacgca
     8941 gctcttcacg ggcacgttta cgctcggctt caatttccgc ctgcgcctgg gccacgattt
     9001 tagtacgttc ctgttctgcc tcagctttcg cttcgtccag aatctgcgag cggcgtttgt
     9061 tcgcctgctc gatgattacc tgggcttccg ctttcgcttt tttcagctgg tcggtcgcgc
     9121 tggcctttgc aaggtcaagg tccttatgtg ctcgttctgc ggaagcaagg ccgtcagcaa
     9181 tttctttttg acgtttttcg atggctgcca ttaatggcgg ccatacgtac ttcatgcaga
     9241 acagaacgaa caggacaaac gcgatggcct ggccgaggat tgttgcgtta agattcacag
     9301 cacaatgcct ctatttagtt aacgttctga tattgctctt taaataaaag caacgcttac
     9361 tacgcgacag cgaacatcac gtacagaccc agacctacag cgatcatcgg gatagcatcc
     9421 accagaccca taacgataaa gaactgagta cgcagcagag gaatcagatc aggttgacgc
     9481 gctgcgcctt ccaggaattt acccccgagg atgccgatac cgatcgcagc accgattgcc
     9541 gccagaccca tcatcacagc ggcagccatg tacagcagat ccatattcag gttttccatg
     9601 acagtctcca gtttgtttca gttaaaacgt agtagtgttg gtaaattaat gttcttcaga
     9661 cgccatcgac agatagacga tcgtcagaac catgaagatg aaggcttgca gcgtaatgat
     9721 caggatgtgg aaaatggccc acggcacatt caggatccac tgtgaccacc acggcaacag
     9781 accagcaatc agaatgaaaa tcagctcacc ggcatacatg ttaccgaaca gtcgcaaacc
     9841 gagtgaaact ggtttggaca gcaggcttac cccttcaagg attaagttga caggaatgaa
     9901 cgcccagtga ttgaacggct gcagcgtcaa ctctttcgtg aagccgccga tgcctttcat
     9961 tttgatgctg tagaacagaa tcaggataaa tacgcccagt gccatagaca gcgttacgtt
    10021 cacgtccgca gacggaacca cacgcagtgc aggcagaccc agtacatgtt cagcaatgta
    10081 cggcagcagg tcgataggca gtaaatccat caggttcatc aggaataccc agacgaagat
    10141 cgtcagggcc agcggagcaa tcagcttgct tttgccatgg tacatgtctt tcacgctacc
    10201 attaacaaag ccgatcacca gctcaatcgc ggtctgaaac ttacctggca caccgctggt
    10261 cgcctttttg gctacgctac ggaataaaac caggaacaac agacccagca ccaccgagaa
    10321 gaacatggag tcaatattga ttgtccagaa ggtggctggg gggttttgtg gatccaccag
    10381 cgagaatgta cgcaggtcca gctgaaggtt attcaggtgg tgtcctatgt aatcctgcgg
    10441 cgtcatattt tctgaagcca tgatgccttt taccctttgt tgttaattac agccggtgcc
    10501 agtatctgaa ccaccagcac caaaacccac gtaacgatca gcggcaagaa taccgccttt
    10561 aaaaccgcca acgccaccac cagtaacacc aacatcgcca gaactttgaa agcttcacca
    10621 aatgcgaatg tccaggccac ccggcctttc gctggtgtat gcgcctggtg acgccaggca
    10681 aatatcataa acaaaacgtt aggcagaaag actgccaggc ccccgcttat tgcagagacg
    10741 ccccagaagg ggtctttgag gctgaacagc aatccacttg ctatcaccac cagtaactga
    10801 acgagcagaa gcttccgagc aacgtttcga ctcacgagcg acacagacat cacgtttttc
    10861 actcctgctc ccttcgaggt atgccgcgtg tcgtataaaa ctttctttaa ggcttagagt
    10921 caagcatcaa aaagcggtca aattatacgg tgcgcccccg tgatttcaaa caataagtag
    10981 ccaaaaggtg aataaatgtt taaatatttt tccagtgcat acaattgcga cttttctact
    11041 aaccctgttc gatcatgaaa aactgtaaat aacgcgtaaa cactggtgat aaagcgtgct
    11101 tcagatcaca tattgcgcat gttcgcgcac agcatattta tttacttggc aaatgatgcc
    11161 tttgcaagtt tatgatattt cagtctaaaa acagatactg ttttaataaa tgacatttac
    11221 acaacaaaaa ccacccattg acatttttaa taatgtttta acagccaatg atggttctta
    11281 gcgccgattt ttagcagact gatattttca ctaatgactt attttctgct taccaaaaaa
    11341 agccacgtta tcttgttgat gcaaaagagt gaacgtggcg ttaaatgtaa ccagttatat
    11401 cagtagaaaa cc
//
LOCUS       DAZKIP010000130        11407 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZKIP010000130 DAZKIP010000000
VERSION     DAZKIP010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.131"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            242..541
                     /gene="yoaC"
                     /locus_tag="ACHOUK_003885"
     CDS             242..541
                     /gene="yoaC"
                     /locus_tag="ACHOUK_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFP9899237.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(661..840)
                     /gene="yoaH"
                     /locus_tag="ACHOUK_003886"
     CDS             complement(661..840)
                     /gene="yoaH"
                     /locus_tag="ACHOUK_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFP9899238.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            914..2275
                     /gene="pabB"
                     /locus_tag="ACHOUK_003887"
     CDS             914..2275
                     /gene="pabB"
                     /locus_tag="ACHOUK_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFP9899239.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRRVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPAPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2279..2857
                     /gene="nudL"
                     /locus_tag="ACHOUK_003888"
     CDS             2279..2857
                     /gene="nudL"
                     /locus_tag="ACHOUK_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFP9899240.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            3041..4405
                     /gene="sdaA"
                     /locus_tag="ACHOUK_003889"
     CDS             3041..4405
                     /gene="sdaA"
                     /locus_tag="ACHOUK_003889"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFP9899241.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     TANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4536..6134
                     /gene="pdeD"
                     /locus_tag="ACHOUK_003890"
     CDS             4536..6134
                     /gene="pdeD"
                     /locus_tag="ACHOUK_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFP9899242.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTTDRVLIKGSPVLIQWTPAASSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFSFTINVNGPGAPALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(6138..7694)
                     /gene="yoaE"
                     /locus_tag="ACHOUK_003891"
     CDS             complement(6138..7694)
                     /gene="yoaE"
                     /locus_tag="ACHOUK_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFP9899243.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(7682..7840)
                     /gene="yoaL"
                     /locus_tag="ACHOUK_003892"
     CDS             complement(7682..7840)
                     /gene="yoaL"
                     /locus_tag="ACHOUK_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFP9899244.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            8157..9128
                     /gene="manX"
                     /locus_tag="ACHOUK_003893"
     CDS             8157..9128
                     /gene="manX"
                     /locus_tag="ACHOUK_003893"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFP9899245.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9191..9991
                     /gene="manY"
                     /locus_tag="ACHOUK_003894"
     CDS             9191..9991
                     /gene="manY"
                     /locus_tag="ACHOUK_003894"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFP9899246.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            10004..10855
                     /gene="manZ"
                     /locus_tag="ACHOUK_003895"
     CDS             10004..10855
                     /gene="manZ"
                     /locus_tag="ACHOUK_003895"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFP9899247.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10910..11368
                     /gene="yobD"
                     /locus_tag="ACHOUK_003896"
     CDS             10910..11368
                     /gene="yobD"
                     /locus_tag="ACHOUK_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFP9899248.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLERIYKLLVSTQ"
BASE COUNT         2800 a         2874 c         2863 g         2870 t
ORIGIN      
        1 ttcgcccttt gattagcaac tctggttttc tttcttagct gaacaatctg gttatagcgt
       61 gcggactgac cgttaatttt ctccatctta ctggataaca caccatgagc tgctaccttt
      121 aaaacacctg aatatcaaca ggttaactcg tgccatatcg ttataatccc tttgatatta
      181 gatgcaaatt aaggtcatat atagccttat cgataacatg gttaatttta aggataagaa
      241 tatgcctgct gtaatagata aagccctgga tttcattggt gccatggatg tatcagcgcc
      301 aacaccaagt tcgatgaatg aaagcacggc gaagggaatc tttaaatatt taaaagaact
      361 gggagtaccc gcgagtgccg ctgatattac ggcgcgagct gaccaggaag gctggaaccc
      421 tgggttcacg gaaaaaatgg ttggatgggc aaaaaaaatg gagacaggtg aacgctctgt
      481 gattaaaaat cctgagtact tttcaacata tatgcaggaa gaactaaaag cactggtctg
      541 agttaaattt atatcagcat aaatgggtca ggacgctttt aatcacatat aaaaagcgtc
      601 ctttttctca caatcaaata atcatcagct tctataacga aatcgatacg gattagtgtg
      661 ttactcgtct tcatcctcaa aacgcgccac gattcgctcg ccggagtggt tggcacgcag
      721 ctcttctgcc accagcgcga ttgcctggcc gctgctcatc ccttgtgcca tcagttcctg
      781 gatccgctcg acagcttttt gctgctgttc atgggtgagt gaaggtaaac ctgcaaacat
      841 tgttaactcc tgctaaattg ttggcgctaa ttatttcatg ctacccggca catagccagt
      901 agagtcagga ctgatgaaga cgttatctcc cgctgtgatt actttaccct ggcgtcagga
      961 cgccgctgaa ttttatttct cccgcttaag ccacctgccg tgggcgatgc ttttacactc
     1021 cggctatgcc gatcatccgt atagccgctt tgatattgtg gtcgccgatc cgatttgcac
     1081 tttaaccact ttaggtaaag aaaccgttgt tagtgaaagc gaaaaacgca caacgaccac
     1141 tgatgacccg ctacaggtgc tccagcaggt gctggatcgc gcagacattc gcccaacgca
     1201 taacgaagat ttgccatttc agggcggcgc actggggttg tttggctacg atctgggccg
     1261 ccgttttgag tcactgccag aaattgcgga acaagatatc gttctgccgg atatggcagt
     1321 gggtatctac gattgggcgc tcattgtcga ccaccagcgt catacagttt ctttgctgag
     1381 tcataatgat gtcaatgccc gtcgggcctg gctggaaagc cagcaattct cgccgcagga
     1441 agatttcacg ctcacttccg actggcaatc caatatgacc cgcgagcagt acggcgaaaa
     1501 atttcgccgg gtacaggaat atctgcacag cggtgattgc tatcaggtga atctcgccca
     1561 gcgttttcat gcgacctatt ctggcgatga atggcaggca ttccttcagc ttaatcaggc
     1621 caaccgcgcg ccatttagcg cttttttacg tcttgaacag ggtgcaattt taagcctttc
     1681 gccagagcgg tttattcttt gtgataatag tgaaatccag acccgcccga ttaaaggcac
     1741 gctaccacgc ctgcccgctc ctcaggaaga tagcaaacaa gcagaaaaac tggcgaactc
     1801 agcgaaagat cgtgccgaaa atctgatgat tgtcgattta atgcgtaatg atatcggtcg
     1861 tgttgccgta gcaggttcgg taaaagtacc agagctgttc gtggtggaac ccttccctgc
     1921 cgtgcatcat ctggtcagca ccataacggc gcaactacca gaacagttac acgccagcga
     1981 tctgctgcgc gcagcttttc ctggtggctc aataaccggg gctccgaaag tacgggctat
     2041 ggaaattatc gacgaactgg aaccgcagcg acgtaatgcc tggtgcggca gcattggcta
     2101 tttgagcttt tgcggcaaca tggataccag cattactatc cgcacgctga ctgccattaa
     2161 cggacaaata tactgctctg cgggaggtgg aattgtcgcc gatagccagg aagaagcgga
     2221 atatcaggaa acttttgata aagttaataa gatattacgc caactggaga agtaagacgt
     2281 ggaataccgt agcctgacgc ttgatgattt tttatcgcgc tttcaacttt tgcgcccgca
     2341 aattaaccgg gaaaccctaa atcatcgtca ggctgccgtg ttaatcccca tcgtccgtcg
     2401 accgcaaccg gggttgttgc tgactcagcg ttcaattcat ctgcgtaaac acgctggaca
     2461 agtggcattc cctggaggtg cagtcgatga cacggacgca tcagttatcg ccgccgcgct
     2521 gcgcgaagct gaagaagagg ttgctatacc gccttccgcc gttgaagtta tcggcgtgct
     2581 gccgcccgtc gatagcgtaa ctggctacca ggtaacccca gtggtcggca ttatcccgcc
     2641 cgatctgccg tatcgcgcca gtgaagatga agtttccgct gtgtttgaaa tgccgctcgc
     2701 ccaggcatta catctgggtc gttatcaccc tttagatatc taccgccgcg gcgattcaca
     2761 tcgggtatgg ctatcctggt acgaacagta ttttgtatgg ggaatgaccg caggcataat
     2821 tcgtgagctg gcgctgcaaa ttggtgtgaa accctgacta tacttatctt tacatctaca
     2881 aaacactact tgagacaatc atcgcaatat tagttaaatc gcggtttttg attagtttaa
     2941 ttcatgtgaa tagttaagcc agtcgccgcg ttccctctta cactatgcgc tgttattagt
     3001 tcgttactgg aagtccagtc accttgtcag gagtattatc gtgattagtc tattcgacat
     3061 gtttaaggtg gggattggtc cctcatcttc ccataccgta gggcctatga aggcgggtaa
     3121 acagttcgtc gatgatctgg tcgaaaaagg cttactggat agcgttactc gcgttgccgt
     3181 ggacgtttat ggttcactgt cgctgacggg taaaggccac cacaccgata tcgccattat
     3241 tatgggtctt gcaggtaacg aacctgccac cgtggatatc gacagtattc ccggttttat
     3301 tcgcgacgta gaagagcgcg aacgtctgct gctggcacag ggacggcatg aagtggattt
     3361 cccgcgcgac aacgggatgc gttttcataa cggcaacctg ccgctgcatg aaaacggtat
     3421 gcaaatccac gcctataacg gcgatgaagt cgtctacagc aaaacttatt attccatcgg
     3481 cggcggtttt atcgtcgatg aagaacactt tggtcaggat actgccaacg aagtgagcgt
     3541 gccgtatccg ttcaaatctg ccaccgaact gctcgcgtac tgtaatgaaa ccggctattc
     3601 gctgtctggt ctcgctatgc agaacgaact ggcgctgcac agcaagaaag agatcgacga
     3661 gtatttcgcg catgtctggc aaaccatgca ggcatgtatc gatcgcggga tgaacaccga
     3721 aggtgtactg ccaggcccgc tgcgcgtgcc acgtcgtgcg tctgccctgc gccggatgct
     3781 ggtttccagc gataaactgt ctaacgatcc gatgaatgtc attgactggg taaacatgtt
     3841 tgcgctggca gttaacgaag aaaacgccgc cggtggtcgt gtggtaactg cgccaaccaa
     3901 cggtgcctgc ggtatcgttc cggcagtgct ggcttactat gaccacttta ttgaatcggt
     3961 cagcccggac atctataccc gttactttat ggcagcgggc gcgattggtg cattgtataa
     4021 aatgaacgcc tctatttccg gtgcggaagt tggttgccag ggcgaagtgg gtgttgcctg
     4081 ttcaatggct gctgcgggtc ttgcagaact gctgggcggt agcccggaac aggtttgcgt
     4141 ggcggcggaa attggcatgg aacacaacct tggtttaacc tgcgacccgg ttgcagggca
     4201 ggttcaggtg ccgtgcattg agcgtaatgc cattgcctct gtgaaggcga ttaacgccgc
     4261 acggatggct ctgcgccgca ccagtgcacc gcgcgtctcg ctggataagg tcatcgaaac
     4321 gatgtacgaa accggtaagg acatgaacgc caaataccgc gaaacctcac gcggtggtct
     4381 ggcaatcaaa gtccagtgtg actaatactt cttactcgcc catctgcaac ggatgggcga
     4441 atttatcccc gctttctcgt ctgctgtaat attccccact acacttccac tgttgcgtca
     4501 ggcgtttgtc gccatacgct tacagagtgg cccgcatgca aaaagcacaa cggatcatta
     4561 aaacctatcg ccgtaatcga atgattgttt gtacgatttg cgcactcgtt acgctcgctt
     4621 cgaccctgag cgtgcgattt atttcacagc gtaacttaaa tcaacaacgg gtagtacaat
     4681 tcgccaatca cgctgtagag gaattagata aagtactgct tcccctacag gcaggtagcg
     4741 aagtcttgct tccgctgatt ggtctgccct gctctgtcgc ccatttgcca ttacgtaaac
     4801 aggcggcaaa actccaaact gtgcgatcca ttggcctggt gcaagacggc acactttatt
     4861 gctccagcat ttttggttat cgcaatgtgc ccgtcgtgga cattctggct gaacttcctg
     4921 caccgcaacc acttttacgc ctgacgaccg accgtgtcct gattaaaggc agtccggttt
     4981 tgattcaatg gacgcctgca gcgagcagta gcaatgctgg ggtcatggag atgattaaca
     5041 tcgacttact gacggcaatg ctgcttgagc cacaactgcc gcaaatcagt agcgccagcc
     5101 tgacggtgga caaacggcat ttgctctatg gtaatgggct ggtagattcc cttccacaac
     5161 ctgaagacaa tgaaaactat caggtttctt cgcaacgctt ttcttttacc attaacgtta
     5221 atggtccggg ggctccggcg ctggcatggc actatcttcc aacacaatta ccgctggcgg
     5281 tactgctaag tttactggtg ggctacatcg cctggctggc gaccgcttac cgaatgagct
     5341 tttcccgcga aatcaatctg ggcctggcgc aacatgagtt cgaattgttc tgtcagcctt
     5401 tgcttaatgc gcgcagccag caatgtattg gtgtagagat tttgctacgc tggaacaatc
     5461 cgcgtcaggg ctggatttca ccggatgtgt ttattcctat cgcggaagaa catcatttaa
     5521 ttgtgccact gacccgctat gtgatggcag aaaccattcg tcagcgccat gttttcccga
     5581 tgagtagtca gtttcatgtt ggcattaacg tcgcacccag ccattttcgc cgtggtgtgc
     5641 tgataaaaga tctcaatcag tactggttta gcgctcaccc gattcagcaa ctgatcctcg
     5701 aaatcaccga acgcgatgcc ttactggatg ttgattatcg gattgcccgc gagctacatc
     5761 gtaaaaacgt caaactggcg attgatgact tcggcaccgg caacagttca ttttcctggc
     5821 ttgaaacatt acgtcctgac gtgctgaaaa ttgataagtc atttaccgca gctataggtt
     5881 ctgacgcggt taactcgacg gtgaccgata tcatcatcgc gctggggcaa agactgaata
     5941 ttgaactggt ggcggagggc gtggaaacgc aagaacaggc gaagtatttg cgccgtcatg
     6001 gcgtgcatat tttgcaaggg tatttgtacg cacagccgat gccgctacgt gattttccca
     6061 aatggctggc gggcagccaa ccgccgcccg cccggcataa tggacatatc acgcccatta
     6121 tgccgttacg ttaacgctta ctcatcttca tcgtgcgccg gttgctcttt aacaatgcga
     6181 accagatcaa cacgataatc attggcttca atgatggtga tatgcagtgg ccctacatca
     6241 atcacatcgc ccacacgggg aatgtgacca tttgccgaga tcacgaggcc cgcgaccgtc
     6301 gcgatatcat cgtcatcggc aaggtgctca acatcaagcg cctgctgcaa ggcatgcaaa
     6361 tctgtaccgc cttttaccag ccagccgtca ccatcagtaa tgatttccgg cgtttcgtca
     6421 gcgtccggga attcacccgc aatggcttcc agcacatcca gcggcgtgac caagccttgt
     6481 accacaccaa actcgttggt cacgataaca aagctcccgc gagcacgacg cagcacgccc
     6541 agcaggttga tcggatcgag ggtttcaggg acgataatcg ccggagacgc cgaagcaatc
     6601 gccgccacat caacgccctc ttccagcgcc accagcagtt ctttagcacg tacgattccg
     6661 atgatttcat ccagttcacc gcgacatacc gggaacaggc tgtgcggtga ggagagcagc
     6721 tgctcgcgga tttcatcgac cccgagatta gcgtcaaccc agcttatttc accgcgcggc
     6781 gtcatgatcc cgcgcagaga acgcgacgcc agcgtcagta cgccgttaat catgtaacgt
     6841 tcttcttcgg caaatgcacc ttccgggatc ggcatcggca tcgggttatc ggcatcgtgc
     6901 tgaacattgg cctgacgttt cccgcccatc aaacgcagga tggcatcggc agtacgcgct
     6961 cgcagcggca aagtcgactg gtgacgaata aagttgcgac gcgcaatctg gttaaacact
     7021 tcgatgatga tcgagaagcc aatcgcggca tacaggtaac ctttcggaat gtggaaaccg
     7081 aaaccttctg ccaccagact cagaccaatc attaacagga agctcagaca gagcaccacc
     7141 accgtggggt gctggttaac gaatcgcgtc agcggtttgg atgccagcaa cataaccgcc
     7201 atcgcaatca ctaccgccgc catcatcacc ggcagatggt taaccatccc tactgcagta
     7261 attaccgcat ccaacgagaa gacggcgtca aggatgacaa tctgtgtgac gaccacccag
     7321 aaactggcgt agcctttacc gtggccggaa tcatgatcgc ggttttccag ccgttcatgc
     7381 agttcggttg ttgctttgaa cagcaagaat atccccccga acaacataat caggtcgcgt
     7441 ccggagaagg agaaatccat gacggtaaat agcggtttgg tcagcgtgac catccatgaa
     7501 atcagcgaca gcagccccag acgcataatc agcgccagtg ataaccccag caaacgcgct
     7561 ttatcgcgtt gttttggcgg cagtttgtca gcaagaatgg cgatgaagac caggttatcg
     7621 atacccagca caatttcgag aacaacaagc gtgagtagcc ccgcccaaat tgaggggtcc
     7681 attaagaatt ccatgacaag ctcctgctta aggaatagct attcgacgcc agaaataatg
     7741 caggcgtaac gacaaaatgc aaacgaaagg tgcggcatag agtgccagaa aggcaggcgt
     7801 taaaaggcct gatgctgaaa tgacgtcggt gacgatccat actgcgggct actgccctat
     7861 actccatgat tgttaaacgg gagttaaaca tatcagagac gcctctgatt tggcaaagat
     7921 ttaccttcct ttgcaaacga atgtgacaag gatattttac ctttcgaaat ttctgctaat
     7981 cgaaagttaa attacggatc ttcatcacat aaaataattt tttcgatatc taaaataaat
     8041 cgcgaaacgc aggggttttt ggttgtagcc cttatctgaa tcgattcgat tgtggacgac
     8101 gattcaaaaa tacatctggc acgttgaggt gttaacgata ataaaggagg tagcaagtga
     8161 ccattgctat tgttataggc acacatggtt gggctgcaga gcagttgctt aaaacggcag
     8221 aaatgctgtt aggcgagcag gaaaacgtcg gctggatcga tttcgttcca ggtgaaaatg
     8281 ccgaaacgct gattgaaaag tacaacgctc agttggcaaa actcgacacc actaaaggcg
     8341 tgctgtttct cgttgataca tggggaggca gcccgttcaa tgctgccagc cgcattgtcg
     8401 tcgacaaaga gcattatgaa gtcattgcag gcgttaacat tccaatgctc gtggaaacgt
     8461 taatggctcg tgatgatgac ccaagctttg atgaactggt tgcgctggca gtagaaacag
     8521 gccgtgaagg cgtgaaagca ctgaaagcca aaccggttga aaaagccgcg ccagcacccg
     8581 ctgccgcagc acccaaagcg gctccaactc cggcaaaacc aatggggcca aacgactaca
     8641 tggttattgg ccttgcgcgt atcgacgacc gtctgattca cggtcaggtc gccacccgct
     8701 ggaccaaaga aaccaatgtc tcccgtatta ttgttgttag tgatgaagtg gctgcggata
     8761 ctgttcgtaa gacactgctc acccaggttg cacctccggg cgtaacagca cacgtagttg
     8821 atgttgccaa aatgattcgc gtctacaaca acccgaaata tgctggcgaa cgtgtaatgc
     8881 tgttatttac caacccaaca gatgtagagc gtctcgttga aggcggcgtg aaaatcacct
     8941 ctgttaacgt cggtggtatg gcattccgtc agggtaaaac ccaagtgaat aacgcggttt
     9001 cggttgatga aaaagatatc gaggcgttca aaaaactgaa tgcacgcggt attgagctgg
     9061 aagtccgtaa ggtttccacc gatccgaaac tgaaaatgat ggatctgatc agcaaaatcg
     9121 ataagtaacg tattgtgttg attatcagtc agttttcaca ctttagtcat acgtaaacag
     9181 gagaagtaca atggagatta ccactcttca aattgtgctg gtatttatcg tagcctgtat
     9241 cgcaggtatg ggatcaatcc tcgatgaatt tcagtttcac cgtccgctaa tcgcgtgtac
     9301 cctggtgggt atcgttcttg gggatatgaa aaccggtatt attatcggtg gtacgctgga
     9361 aatgatcgcg ctgggctgga tgaacatcgg tgctgcagtt gcgcctgacg ccgctctggc
     9421 atctatcatt tctaccattc tggttatcgc aggtcatcag agcattggtg caggtattgc
     9481 actggcaatc cctctggccg ctgcgggcca ggtactgacc atcatcgttc gtactattac
     9541 cgttgctttc cagcacgctg cggataaggc tgctgataac ggcaacctga cagcgatttc
     9601 ctggatccac gtttcttctc tgttcctgca agcaatgcgt gtggctattc cggccgtcat
     9661 cgttgcgctg tctgtaggta ccagcgaagt acagaacatg ctgaatgcga ttccggaagt
     9721 ggtgaccaat ggtctgaata tcgccggtgg catgatcgtg gtggttggtt atgcgatggt
     9781 tatcaacatg atgcgtgctg gctacctgat gccgttcttc tacctcggct tcgtaaccgc
     9841 agcattcacc aactttaacc tggttgctct gggtgtgatt ggtactgtta tggcagtgct
     9901 ctacatccaa cttagcccga aatacaaccg cgtagccggt gcgcctgctc aggcagctgg
     9961 taacaacgat ctcgataacg aactggacta acaggtgagc gaaatggttg atacaactca
    10021 aactaccacc gagaaaaaac tcactcaaag tgatattcgt ggcgtcttcc tgcgttctaa
    10081 cctcttccag ggttcatgga acttcgaacg tatgcaggca ctgggtttct gcttctctat
    10141 ggtaccggca attcgtcgcc tctaccctga gaacaacgaa gctcgtaaac aagctattcg
    10201 ccgtcacctg gagttcttta acacccagcc gttcgtggct gcgccgattc tcggcgtaac
    10261 cctggcgctg gaagaacagc gtgctaatgg cgcagagatc gacgacggtg ctatcaacgg
    10321 tatcaaagtc ggtttgatgg ggccgctggc tggtgtaggc gacccgatct tctggggaac
    10381 cgtacgtccg gtatttgcag cactgggtgc cggtatcgcg atgagcggca gcctgttagg
    10441 tccgctgctg ttcttcatcc tgtttaacct ggtgcgtctg gcaacccgtt actacggcgt
    10501 agcgtatggt tactccaaag gtatcgatat cgttaaagat atgggtggtg gcttcctgca
    10561 aaaactgacg gaaggggcgt ctatcctcgg cctgtttgtc atgggggcat tggttaacaa
    10621 gtggacacat gtcaacatcc cgctggttgt ctctcgcatt actgaccaga cgggcaaaga
    10681 acacgttact actgtccaga ctattctgga ccagttaatg ccaggcctgg taccactgct
    10741 gctgaccttt gcttgtatgt ggctactgcg caaaaaagtt aacccgctgt ggatcatcgt
    10801 tggcttcttc gtcatcggta tcgctggtta cgcttgcggc ctgctgggac tgtaagactg
    10861 ttgtacacta ccggggcctt ttggccccgt ttttttatct ggaggattaa tgacaatcac
    10921 ggacctggta ctgattcttt tcatcgccgc actcctggcc ttcgcgatct acgatcagtt
    10981 catcatgccc cgccgtaacg gccccaccct gctggcaatt cctttgctcc ggcgtggtcg
    11041 catcgatagc gttatcttcg tcggattgat tgtcattctt atctataaca acgtcacgaa
    11101 tcatggtgcg ttaataacga catggttatt aagcgcactg gctctgatgg gtttttatat
    11161 attctggatc cgcgttccga agatcatctt taaacaaaaa ggttttttct tcgccaatgt
    11221 ctggattgaa tatagccgaa tcaaagcgat gaacttgtcg gaagatggcg tgttggtgat
    11281 gcaattagaa cagcgtcggc tgttaatccg cgttcgaaat atcgacgatc tggaaagaat
    11341 ttataaactt ctcgtttcaa ctcaatgagt tattaattta gccaaagcta tgtttagtgt
    11401 attttta
//
LOCUS       DAZKIP010000131        11393 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZKIP010000131 DAZKIP010000000
VERSION     DAZKIP010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.132"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            275..1663
                     /gene="narU"
                     /locus_tag="ACHOUK_003897"
     CDS             275..1663
                     /gene="narU"
                     /locus_tag="ACHOUK_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFP9899249.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWKNKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAR
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRMKMKGGSDEQA
                     QREAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1745..5485
                     /gene="narZ"
                     /locus_tag="ACHOUK_003898"
     CDS             1745..5485
                     /gene="narZ"
                     /locus_tag="ACHOUK_003898"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFP9899250.1"
                     /translation="MSKLLDRFRYFKQKGDTFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKSLSYKQVRGRGGFIR
                     SNWPELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGKETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLAGGSTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLSPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRICPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5482..7026
                     /gene="narH"
                     /locus_tag="ACHOUK_003899"
     CDS             5482..7026
                     /gene="narH"
                     /locus_tag="ACHOUK_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFP9899251.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIDAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSIAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7026..7721
                     /gene="narW"
                     /locus_tag="ACHOUK_003900"
     CDS             7026..7721
                     /gene="narW"
                     /locus_tag="ACHOUK_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFP9899252.1"
                     /translation="MQILKVIGLLMEYPDEMLWECKEDALALIRRDAPMLTDFTRNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     TLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7718..8398
                     /gene="narI"
                     /locus_tag="ACHOUK_003901"
     CDS             7718..8398
                     /gene="narI"
                     /locus_tag="ACHOUK_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFP9899253.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8477..9370
                     /locus_tag="ACHOUK_003902"
     CDS             8477..9370
                     /locus_tag="ACHOUK_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFP9899254.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTVWQTSLAGK
                     HRVTIEKHHDGYRISLEQGTPGFEPPLEGETRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLRVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGAAVILFHAEWAIKL"
     gene            complement(9466..10310)
                     /gene="nhoA"
                     /locus_tag="ACHOUK_003903"
                     /pseudo
     CDS             complement(9466..10310)
                     /gene="nhoA"
                     /locus_tag="ACHOUK_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
     gene            10609..11052
                     /locus_tag="ACHOUK_003904"
     CDS             10609..11052
                     /locus_tag="ACHOUK_003904"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFP9899255.1"
                     /translation="MPVEFEPPRVTIVVDKSTWTRELIERNGKFGIVIPGVAATNWTW
                     AVGSVSGREEDKFNCYGIPVVRGPVFGLPLVEEKCLAWMECRLLPATSAQEEYDTLFG
                     EVVSAAADARVFVEGRWQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11056..11283)
                     /gene="pptA"
                     /locus_tag="ACHOUK_003905"
     CDS             complement(11056..11283)
                     /gene="pptA"
                     /locus_tag="ACHOUK_003905"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HFP9899256.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
BASE COUNT         2685 a         2962 c         3007 g         2739 t
ORIGIN      
        1 agttaatata caaccctttg gattcatcat ctgaagggtt gaatcatgac atgcgtacat
       61 agcttaaccc cgtttaatgg ttagcttgtt agttacattt agtaacacat aactggctgc
      121 ttaatcccca ttctgccccc gcggttacgc tttcttatct attctttcaa tcagcacttc
      181 acccatttca tccttaattc taataactgg ctattgcgcc ttacctcctg ttttgtcttt
      241 ttttcattac caatgtgtac atgtgaggaa caatatggca ctgcaaaatg agaaaaatag
      301 tcgttatctt ttacgcgact ggaaaccaga aaatccggcc ttctggaaaa ataaaggaaa
      361 acatattgct cgaagaaacc tctggatatc agtcagttgt ctacttcttg ccttctgtgt
      421 ctggatgcta tttagcgcag ttaccgttaa tctcaataaa atcggtttta atttcactac
      481 cgatcaactc tttttattaa ccgcattacc ctccgtttct ggcgcattat tacgtgtccc
      541 ctactccttt atggtgccta tattcggtgg acgtcgatgg acggttttta gtactgcaat
      601 cctgattatt ccttgcgtct ggctcggaat tgccgtgcag aatccgaata ctccttttgg
      661 gatatttatc gttatcgctt tgctatgcgg ttttgcaggt gcgaactttg cttcgagcat
      721 gggcaatatc agtttcttct ttccaaaagc caggcaaggt agcgctcttg ggattaatgg
      781 cggattagga aacttaggtg taagtgtaat gcagctggtt gcaccgctgg tcatttttgt
      841 acccgtattt gcctttctcg gcgtcaatgg agtaccgcag gccgacggtt cggtgatgtc
      901 gctggcgaat gccgcatgga tttgggtacc gctactagcg attgccacga tcgccgcctg
      961 gtcagggatg aatgatatcg ccagttcacg cgcgtcaatt gccgaccagc tccctgtctt
     1021 acaacgcctg catctctggc tgctgagcct gctttacctt gccaccttcg gttcgtttat
     1081 cggtttttct gcgggtttcg ccatgctggc aaaaacccag ttcccggatg tgaatattct
     1141 gcgcctggcg ttctttggcc catttatcgg tgccatcgcg cggtcggtgg gtggtgctat
     1201 ttccgataaa ttcggcggcg tgcgggtgac gttgatcaac tttattttta tggcgatttt
     1261 cagcgccctg ctgttcctta ccttaccggg cacaggctcc ggtaacttca tcgcctttta
     1321 cgccgtattt atggggctgt ttctgaccgc gggtctggga agtggttcta ctttccagat
     1381 gatcgccgtc atctttcgcc agataaccat ttatcggatg aagatgaaag gcggtagtga
     1441 tgagcaagct cagagagaag ccgtcaccga aacggcggcg gctctgggct ttatctcagc
     1501 catcggcgca gtgggcggct tttttattcc gcaggcattt ggcatgtcac tcaatatgac
     1561 cggctctcca gtgggcgcga tgaaagtgtt tttaatcttc tacatcgttt gtgtgctact
     1621 gacctggctg gtttatggtc ggcggaagtt cagccaaaaa taagagatcg atgcctgatg
     1681 tacaaccgtg tcgcatccga caaggcatca gatcccaatt cattcctgga gcaggagtca
     1741 tgtcatgagt aaacttttgg atcgctttcg ctacttcaaa caaaagggcg atacctttgc
     1801 cgatggtcac ggacaggtga tgcatagcaa ccgcgactgg gaggacagct atcgccagcg
     1861 ttggcagttc gacaaaatcg tgcgttccac ccacggtgtt aactgtacag gctcctgtag
     1921 ctggaaaatc tacgttaaaa atggtctggt gacctgggaa atccaacaga ccgactaccc
     1981 gcgcactcgc cctgacctgc ccaatcatga acctcgcggc tgcccgcgtg gcgcaagtta
     2041 ctcctggtat ctttacagcg ctaaccgcct gaaatacccg ctcattcgta aacgactgat
     2101 tgaactgtgg cgcgaagccc tcaaacaaca cagcgatccg gtactggcgt gggcatcgat
     2161 tatgaacgat ccgcaaaaga gcctgagcta caaacaagtg cgtgggcgcg gcgggtttat
     2221 ccgctccaac tggccggaac taaaccagct gattgccgcc gctaacgtct ggactatcaa
     2281 aacctacggc ccggatcgcg ttgccggttt ctcgccgatc ccggcgatgt cgatggtttc
     2341 ttacgccgcc ggaacacgtt atctgtcact attaggtggc acctgtttaa gcttctacga
     2401 ctggtattgc gacctgccgc ccgcctcgcc gatgacctgg ggcgagcaaa ccgacgtacc
     2461 ggaatctgcc gactggtata actccagtta catcatcgcg tgggggtcta acgtaccgca
     2521 gacacgtacg ccggacgccc atttctttac cgaagtacgc tacaaaggca ctaaaaccat
     2581 cgccattacc cctgactact ctgaagtggc caaattgtgc gaccagtggc tggcaccaaa
     2641 acaaggcact gatagcgccc tggcgatggc aatgggccat gtgattttaa aagagtttca
     2701 tctcgataat cccagcgact actttatcaa ctactgccgc cgctatagcg acatgccgat
     2761 gctggtaatg ctggaacctc gcgacgatgg tagctacgta cccgggcgca tgatccgcgc
     2821 gtctgacctg gtagatggac taggcgaaag caataatccg cagtggaaaa ccgtggcggt
     2881 taataccgca ggtgaattgg tagtgccgaa tggttcgatt ggtttccgct ggggagaaaa
     2941 aggcaaatgg aatctggaat ccattgccgc cggtaaggaa accgaattgt cgttaaccct
     3001 gctcggtcaa catgacgccg ttgcaggcgt ggctttcccc tactttggcg gcatcgaaaa
     3061 tccgcatttt cgcagcgtca aacacaatcc tgtcctggtg cgtcaattac ccgttaaaaa
     3121 cctgacatta gccggtggca gcacctgtcc agtggtcagc gtttatgatt tggtactggc
     3181 gaattacggc ctcgatcgcg ggctggaaga tgaaaacagc gcgaaagatt acgctgaaat
     3241 caaaccgtac accccagcct ggggtgagca aattaccggc gtgccgcgcc agtatattga
     3301 aaccatcgct cgtgaatttg ccgatactgc ccataaaacg catgggcgct cgatgattat
     3361 cctcggcgca ggtgttaacc actggtatca catggacatg aactaccggg gaatgatcaa
     3421 tatgctgatc ttctgcggtt gtgtcggaca aagcggtggc ggctgggcac actatgtcgg
     3481 tcaggaaaaa ctgcgcccgc aaaccggctg gttgccgctg gcctttgcgc tcgactggaa
     3541 ccgaccaccg cgccagatga acagcacctc gtttttctac aatcattcca gccaatggcg
     3601 ctatgaaaaa gtctctgcgc aggagttact ttcaccgctt gccgatgcca gtaagtacag
     3661 cggtcatctg attgatttta acgttcgcgc cgaacgcatg ggctggctac cttctgcgcc
     3721 gcagttgggg cgtaacccgc tcgggattaa agctgaagcc gacaaggccg gattatcccc
     3781 cacagaattt accgcccagg cgctgaaatc gggcgattta cgtatggcct gcgaacaacc
     3841 agatagcggc agcaatcatc cgcgtaattt gtttgtctgg cgttctaacc tgcttggctc
     3901 ctccggcaaa ggccacgagt atatgcagaa gtatctgctg gggaccgaaa gcgggattca
     3961 gggcgaggaa ctcggtgcca gcgacgggat caaaccggaa gaagtcgagt ggcaaactgc
     4021 ggcgattgaa ggcaagctcg acctgctggt gacgctcgac ttccgcatgt ccagtacctg
     4081 cctgttctcc gatatcgttc tgcccaccgc cacctggtat gaaaaagacg atatgaacac
     4141 ctcggatatg catccattta ttcatccgct ttctgcggcg gtcgatccgg cctgggaatc
     4201 acgcagcgac tgggaaatct acaaaggtat tgccaaagca ttttcgcaag tgtgcgtggg
     4261 ccatcttggc aaagaaaccg acgtggtatt acaaccactg ctgcatgact ctccggcaga
     4321 gctctcacag ccgtgtgaag tgctcgactg gcgcaaaggc gaatgcgatc tgagcccggg
     4381 taaaaccgcg ccgaatattg tggcggtgga gcgcgactac cctgctacgt atgaacgctt
     4441 tacctcgctc gggccattga tggacaaact tggcaacggc ggtaaaggga tttcgtggaa
     4501 tacgcaggat gaaatcgatt tcctcggtaa actcaattac accaagcgtg atggcccagc
     4561 gcaggggcgt ccgctgattg acaccgccat tgacgcttca gaagtgattc tggcactggc
     4621 accagaaacc aacggtcatg ttgcagttaa agcgtggcag gcgctgggcg agatcaccgg
     4681 acgcgaacat acccatctgg cgctgcacaa agaggacgag aagattcgct ttcgcgatat
     4741 tcaggcgcag ccgcgtaaaa ttatctccag ccccacatgg tctggtctgg aaagcgatca
     4801 cgtctcctat aatgcgggat acaccaacgt tcatgagtta attccgtggc gcacgctgtc
     4861 gggacgccag cagctctatc aggatcatcc gtggatgcgt gcttttggtg aaagcctggt
     4921 ggcatatcgc ccgcctatcg acacccgtag cgtcagtgag atgcgccaga tcccgccaaa
     4981 cggcttcccg gaaaaagcac ttaacttcct gacgccgcac cagaaatggg gcattcactc
     5041 aacctacagt gaaaacctgc taatgctgac gctctctcgc ggtggaccga ttgtctggat
     5101 cagcgaaacc gatgcccgtg aactaaccat tgtcgataac gactgggtgg aagtgtttaa
     5161 cgccaatggc gcgctgacgg cccgcgcggt ggtcagccaa cgtgtaccgc cgggtatgac
     5221 catgatgtat cacgctcagg aacgcattat gaatattcct ggttcggaag taactggcat
     5281 gcgcggcggt attcataact cggtcacccg catttgcccg aaaccaacgc atatgattgg
     5341 cggttacgcg cagctggcct ggggctttaa ctactacggc accgtcggct cgaaccgcga
     5401 cgaattcatc atgatccgca agatgaagaa cgttaactgg ctggatgatg aaggtcgcga
     5461 tcaggtacag gaggcgaaaa aatgaaaatc cgttcgcaag tcggcatggt gcttaatctc
     5521 gataaatgta tcggctgcca tacctgttcg gtgacctgta aaaacgtctg gaccggacgc
     5581 gaaggcatgg agtacgcatg gtttaacaac gtcgaaacca aaccgggcat tggttatccg
     5641 aaaaactggg aagatcagga agagtggcaa ggcggctggg tccgcgatgt gaatggcaag
     5701 atacgcccgc gtctgggcag caagatgggt gtaataacca aaatcttcgc caacccggtg
     5761 gtgccgcaga ttgatgatta ctacgaacct ttcaccttcg actacgaaca tttgcatagc
     5821 gcaccggaag gcaaacatat ccctactgct cgcccgcgtt cgctgattga cggtaagcgg
     5881 atggacaaag tgatctgggg gccaaactgg gaagaactgc tgggcggcga gtttgaaaaa
     5941 cgtgcccgcg accgcaactt cgaggccatg caaaaggaga tgtacgggca gtttgaaaac
     6001 accttcatga tgtacctgcc gcgcctgtgc gaacactgcc tcaatcccag ctgcgtggcg
     6061 acctgcccaa gcggcgctat ctacaaacgc gaagaagacg gcattgtgct gattgatcag
     6121 gataaatgcc gtggctggcg tttgtgcatt agcggttgtc cgtacaaaaa aatctacttc
     6181 aactggaaaa gcggcaagtc agaaaaatgt atcttctgtt acccacgaat tgagtccggt
     6241 caaccgaccg tgtgctcaga aacctgcgtg ggtcgcatcc gatatctggg cgtgctgctt
     6301 tacgacgctg accgcatcga ggaagcggcg agcaccgagc gcgaggttga cctctatgaa
     6361 cgccagtgcg aagtgttcct cgatccgcac gatccctcag tgatcgagga agccctgaaa
     6421 caaggtattc cacaaaatgt gattgacgct gcccagcgtt cgccagtcta caaaatggcg
     6481 atggactgga aactggcact accgctgcac cctgaatacc gcaccctgcc gatggtctgg
     6541 tacgttcctc cgctgtcacc gattcagtcc tacgcagatg cgggcggttt gccgaaaagc
     6601 gaaggcgtgc tgcccgccat cgaaagcctg cgtattccgg tgcaatatct cgccaatatg
     6661 ttgagtgccg gcgataccgg tccggtactg cgggcgctga aacggatgat ggcgatgcgc
     6721 cactatatgc gttcacaaac cgtggaaggc gttactgata ctcgtgccat cgacgaagta
     6781 ggcctgagca tcgcccaggt cgaagaaatg tatcgctatc tcgccattgc caactatgaa
     6841 gatcgttttg ttatcccgac cagccatcgg gaaatggcgg gcgatgcctt cgcagaacgc
     6901 aatggctgcg gttttacctt tggtgacggt tgtcacggct cggacagcaa attcaacctg
     6961 ttcaacagta gccgtatcga tgccatcaac atcaccgaag tgcgcgacaa agcggagggc
     7021 gaataatgca gatcctcaaa gtgatcggcc tgttgatgga gtatccggac gagatgttgt
     7081 gggaatgcaa ggaggacgcg ctggcgttga tccgccgcga tgcgccgatg cttacggatt
     7141 tcacccgcaa cctgcttaac gcgccgctgc tggataaaca ggccgaatgg tgcgaagtgt
     7201 ttgaccgcgg gcgcaccacg tcgctgctgc tgttcgaaca tgttcatgcc gagtcccgcg
     7261 atcgcggcca ggcaatggtg gacctgctgg cggagtatga aaaggtcggc ctgcagctgg
     7321 attgtcggga actgcccgat tatctaccgc tgtatctgga gtatttaagc gtactgcctg
     7381 acgatcaggc gaaagaagga ttgctcaacg ttgcgccgat cctcgccctg cttggcggtc
     7441 gcttaaaaca acgcgaggca ccgtggtacg cgttgtttga tgctctgctg caactggcgg
     7501 gaagtacact ttcaagtgac agtgtcacaa aacaagtaaa cagcgaagag cgcgatgaca
     7561 cccgccaggc gcttgatgca gtgtgggaag aggaacaggt gaagtttatt gaagataacg
     7621 ccacggcgtg tgacagctcg ccgcttaatc aatatcagcg acgctttagt caggatgtcg
     7681 cgccgcaata tgtcgacatc agtgcgggag gtgggaaatg attcagtatc tgaacgtctt
     7741 tttttacgat atctacccgt acatttgtgc gacggtgttt ttcctcggca gctggctgcg
     7801 ctacgactac gggcagtaca cctggcgcgc ctcctcaagt cagatgctcg ataaacgcgg
     7861 gatggtgata tggtcgaatc tgttccatat cggcattttg gggatcttct tcgggcacct
     7921 gttcggcatg ttaacgccac actggatgta cgcgtggttt ttgccagtgg cagcgaaaca
     7981 actaatggcg atggtgctcg gtggtatttg cggtgtgttg acgttgattg gcggcgcagg
     8041 actgctgtgg cgcaggctga caaatcagcg cgtgcgtgcc acttccacta cgccggatat
     8101 catcatcatg agcattttgc tgatccagtg tctgttgggg ctaagtacca taccgttctc
     8161 agcacagtat cctgatggta gcgaaatgat gaagctggtg ggttgggcgc aaagcattgt
     8221 cactttccgt ggtggatcgt cagagatgct taacggtgta gcgttcgtct tccgcctgca
     8281 tctggtgttg ggaatgacca tcttcttgct cttcccattc acccgactgg tgcatgtgtg
     8341 gagcgccccg tttgagtact ttactcgtcg gtatcagatt gtgcgctcgc ggcgataatt
     8401 tcaccctggc cggatagccc ttcgctctcc ggccacttgc aggctatgat agttctccag
     8461 tcacaggaga acgttaatga aaccgcaggt ttaccacgtc gatgctttta cctctcaacc
     8521 atttcgcggc aattctgccg gtgttgtttt ccccgccgat aatcttagcg aagcgcaaat
     8581 gcagcttatc gcccgcgaat taggtcattc ggaaaccgct tttctgctgc acagcgacga
     8641 cagcgatgtg cgcatccgct actttacgcc aacagttgaa gtgcctattt gcggtcacgc
     8701 gacagtagct gcgcactatg tacgtgcgaa ggtgttaggt ttaggaaatt gcacggtctg
     8761 gcaaacatcg ctggcaggaa aacatcgcgt gactatcgaa aagcaccacg atggttatcg
     8821 tatttcgctg gaacaaggta cgccaggctt tgagccaccg ctggaaggtg aaacacgtgc
     8881 ggcgattatc aacgcgcttc atctcactga ggacgatatt ctgccaggct tgccaattca
     8941 ggtggcaacc acaggacact caaaagtgat gatcccactg aaaccggaag tggatatcga
     9001 cgccctttcg cctgacctta atgcgctgac cgctatcagc aaacagattg gctgcaatgg
     9061 tttcttcccg ttccagatcc gcccaggcaa aaacgaaacc gatggtcgca tgttctcgcc
     9121 tgcgattggt attgtggaag atccggtgac tggcaatgcc aatggcccga tgggtgcatg
     9181 gttggtacat cacaacgtat tgccccacga tggcaacgtg ttgcgtgtta aaggccatca
     9241 ggggcgcgca ttggggcgtg acggtatgat tgaagtgacg gtgacaattc gtgataacca
     9301 accggaaaaa gtcaccattt ctggcgcggc ggtgattttg tttcatgccg aatgggcaat
     9361 taaactctga aaccgttgcc tgatgcgctt cgcctgtcag gtctacaacc gggctaatgt
     9421 atgccggata aggcgtttac gccgcatccg gcaacctgac ataaattatt ttcccgcctc
     9481 cgggtgcgta tcaaacgccg ccatcaccag cgctaactca tccacggtaa agccatgttt
     9541 cgcatcatcc acgcccagac caaactgttc ttgcatcaca gcatataacg acgccacatc
     9601 cggtagattt cgctgctcca ccgcgtgccc attttcataa tgggaaaatg aaaattggtc
     9661 agtgtcagct tgccgccgtc cggcaaatgg cggcacatca gcaaatgatg gcgaaaatgc
     9721 gactgcggcc agtgcgccga ccagaaatta cccatcacat aatcgctttg ttgctgctcg
     9781 cagagatcaa aacggtacat cgactgccag tgctgatgat gattaaactg caacacccaa
     9841 tcatcaccct cctgcaacaa ccgatactct ccgtgtggcg tggtctgcac aagatcggga
     9901 actaaacgaa tcggcgcggt tagcgtctgc ccaccgaaac cgacatcagc aatccatttt
     9961 tcctcttcca gttccaccaa cagcaaacga tgggtgcgcg gcggtaatgc tggcggattt
    10021 gataacacta cccgccctaa caagctgcga acgttaaacc ccagctcgcg taacacccgc
    10081 tcaaaaacgc cattctgctc aaaacagtaa ccgccacgac gggctatcac cagtttctct
    10141 tccagcgatt gatcatcaag ctgcatttcc ctcggcagca aaacgtcgag gttttcaaac
    10201 ggaatggtgc aattgtgttt caggtgcaat gcacgaagcg tatcaatatt gaccgcagca
    10261 gctcccgacc agttaatacg ggcaaaatag tgattcagaa tgggcgtcat caggcgtttc
    10321 ccttagtggt tttctcaact tataacctgg aatagacgcc gcaccggcta ttttgtgcga
    10381 tcgcggacat gcatcacgtt cgcaaatcgc caatcccgtt aatttcgtta cttattgcgt
    10441 tacggactac gcttaaaaaa accttcttcg cgaggcgata ccatgagccg attcatcccc
    10501 atagaattac accatgccag ccggctgtta aatcatggcc caactgtcat gatcaccagt
    10561 ttcgatgagc aatcccagcg gcgtaacatt atggctgcag cctgatcaat gccggtggag
    10621 tttgaacctc cacgcgtgac gattgtggta gataaatcga catggaccag ggagttgatt
    10681 gaacgtaacg gtaaatttgg catcgttatc ccgggcgttg cagcaactaa ctggacgtgg
    10741 gcagtgggaa gtgtgtcggg tcgtgaagaa gataaattta attgctatgg cattccggtt
    10801 gtgagaggcc cggtatttgg tttgcctctg gtcgaggaaa aatgtctggc gtggatggag
    10861 tgtcgattgc tacctgcgac ttctgcgcaa gaagaatacg acacgctgtt tggcgaagta
    10921 gtatcagcag cggcagacgc acgggtattt gtcgaaggcc gctggcagtt tgatgatgat
    10981 aagctcaata cgttgcatca tttaggtgct gggacgtttg ttaccagcgg caagcgtgtt
    11041 acggcgggtt aagcattaca tgctgtaacc gggtttcttt atcaaagcct ccatttgggg
    11101 cgcgatttcg gcatcccaga tagcttgcca agattctggt tgaatctgtt gtagagcaat
    11161 gcttatcgaa ctgtctttac tgttcagatg gcgaataata acgtcggtaa tatctgcagc
    11221 aagtgctgct ttttgttgtt cgtccagttc acgcggaaaa catttaatgt ctatgtgcgg
    11281 catgattacg ctccttttga aagatgtcag taaaattaac acacattgtg aatgtcgtgt
    11341 gtccggtaaa gcgtttttac gacaataatg ccagggcaag attacgaaag ccc
//
LOCUS       DAZKIP010000132        11340 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZKIP010000132 DAZKIP010000000
VERSION     DAZKIP010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.133"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(150..326)
                     /locus_tag="ACHOUK_003906"
     CDS             complement(150..326)
                     /locus_tag="ACHOUK_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899257.1"
                     /translation="MEKDLKELREYLLLSPVYSEINDCLASLSMEDVTNEEFAERLGI
                     APDWMDAIDTSQLA"
     gene            629..1255
                     /locus_tag="ACHOUK_003907"
     CDS             629..1255
                     /locus_tag="ACHOUK_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9899258.1"
                     /translation="MNVRIYCRASTEGQHADRALTSLREFSKSKGWQIAGEYIENASG
                     AKLERVELMRLLSEAQSGDLLLVEAIDRLSRLEHSAWVELKDTLNRKGLIIVSMDLPT
                     SWQMVEMAGNDLTSGILRAVNAMLIDILATMARQDYETRRKRQQQGIERAKSEGIYIG
                     RAKNQEAREIVREMLEQGVKPELIMKAAGISRATYYRIKNELLIVKSE"
     gene            1606..1773
                     /locus_tag="ACHOUK_003908"
     CDS             1606..1773
                     /locus_tag="ACHOUK_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001316510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFP9899259.1"
                     /translation="MLHSAMHSLMNSQARDTRDRSQNDDNVLEISEPCIRSTFERLYQ
                     NMNEVFGLGDY"
     gene            complement(1853..3100)
                     /locus_tag="ACHOUK_003909"
     CDS             complement(1853..3100)
                     /locus_tag="ACHOUK_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFP9899260.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(3172..4086)
                     /locus_tag="ACHOUK_003910"
     CDS             complement(3172..4086)
                     /locus_tag="ACHOUK_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFP9899261.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGT
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTESRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDRDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVDCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            4302..5735
                     /gene="cscA"
                     /locus_tag="ACHOUK_003911"
     CDS             4302..5735
                     /gene="cscA"
                     /locus_tag="ACHOUK_003911"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFP9899262.1"
                     /translation="MTQSRLHAAQNALAKLHERRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDENDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFVAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSISPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVVQHGALWQLG"
     gene            complement(5743..6738)
                     /locus_tag="ACHOUK_003912"
     CDS             complement(5743..6738)
                     /locus_tag="ACHOUK_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9899263.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGSVTRIPCPLLIRCST"
     gene            6981..7199
                     /locus_tag="ACHOUK_003913"
                     /pseudo
     CDS             6981..7199
                     /locus_tag="ACHOUK_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            7217..8545
                     /gene="dsdA"
                     /locus_tag="ACHOUK_003914"
     CDS             7217..8545
                     /gene="dsdA"
                     /locus_tag="ACHOUK_003914"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFP9899264.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLEDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAEQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMEQYLAKGR"
     gene            complement(8653..10191)
                     /gene="emrY"
                     /locus_tag="ACHOUK_003915"
     CDS             complement(8653..10191)
                     /gene="emrY"
                     /locus_tag="ACHOUK_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFP9899265.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     SINLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVIFLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTETIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(10191..>11340)
                     /gene="emrK"
                     /locus_tag="ACHOUK_003916"
     CDS             complement(10191..>11340)
                     /gene="emrK"
                     /locus_tag="ACHOUK_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFP9899266.1"
                     /translation="SNKKHSNRRKYFSLLAIVLFIAFSGAYAYWSMELEDMISTDDAY
                     VTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLANIVRQ
                     TNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTLISSKA
                     ALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGYIAQRS
                     VQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENVVFHGR
                     VTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSMTATID
                     TKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
BASE COUNT         3249 a         2708 c         2565 g         2818 t
ORIGIN      
        1 atttcaatta ggtttaaaaa ttgtgatact tatgacagat ttgagtgaca ttttatcaat
       61 ctactgattt tgatggtgaa atttattaga aggtatgcgt agtgctacgg gagaaatgag
      121 aaacgcgccc agcaatggcg catcctttct taagcaagtt ggctagtatc tatagcatcc
      181 atccaatcag gagcaattcc caaacgttcc gcgaactctt cattggtcac gtcttccatt
      241 gagagactag ccagacaatc gttaatttca gaatagacgg gggaaagtaa taggtattcc
      301 cgcaactctt tcaggtcttt ttccatctta acactccttg ttaatagacc gtcgaacaat
      361 ggtacagatt tgtaccattc caagtactgg caagcaaccc caccagcaca acaaattatc
      421 tcatacggta aaaacggtgt cgggctgttt ctttcggcta gctcaatata tcaccttaaa
      481 caccagccgt tatcattgtc tcaattgggt ataccgtaat gacaaggtga tacaaagtct
      541 caatagggtt gtataggaac attttgacta cacaaactgc attatgtatc atttgcactt
      601 atattcacaa cccttttgag acaatgcaat gaacgttaga atttactgcc gagcatcaac
      661 tgaggggcaa cacgctgatc gagcacttac cagcctacgt gaattttcaa aatcaaaagg
      721 ctggcagatt gcaggagagt atatagaaaa tgctagcggg gcgaagcttg agcgcgtaga
      781 gcttatgcgg ctattgtctg aagcccagtc cggcgatctg ttgctagtgg aagctataga
      841 tcgtctgagt cgcctagaac atagtgcatg ggtcgaactg aaagatacgc tcaaccgaaa
      901 agggttaatc atcgtatcaa tggatttacc aacgagctgg cagatggtcg agatggcagg
      961 caatgatcta accagcggca tcctgagagc cgtaaatgcc atgttgattg atatccttgc
     1021 cacaatggca cggcaggatt acgaaacacg ccgtaaacgc caacagcaag gcattgaacg
     1081 agctaaaagc gaaggcatat acattgggag ggcgaaaaat caggaagccc gagagatcgt
     1141 tagagagatg ttggagcaag gagtaaaacc tgaactcatc atgaaggcag caggaatcag
     1201 ccgagcgact tactaccgga taaaaaacga gctattgatc gtaaaatcag aataaaattt
     1261 gaaaggtcgt caggcttgtt tcatcaggta aaaaccagca tttctgatca caacgcttgg
     1321 gggcacctat tggggcactc accaaaatcg aaagagaaat aaactacaat tcaaattgtt
     1381 agctaatttt tcgattcctg cagggacacc agataccctt caaaccaaat ctaccttcat
     1441 cccgtaaacg atgggtttgg cagcacactt gccctatatc tactcatttt tactgcaaca
     1501 ggttgaaatc tcagcactgt cagaaagcgc tgatgactaa acagcccagg gccgggcgat
     1561 gtaaccatca cacagaatcc tgatagcgaa atacggcgtg actcgatact tcactccgca
     1621 atgcattcct tgatgaattc gcaggcccgt gatacacggg acaggtcgca gaatgacgac
     1681 aatgtcctgg aaatcagcga accgtgtatc cggagtacat ttgagcgact gtaccagaac
     1741 atgaatgagg tgtttggatt aggcgattat tagcagggct aagcatttta gtattattat
     1801 tttccggttg aaggatatgg agatatcgac aacaaccgga aaaagtttac gtctatattg
     1861 ctgaaggtac aggcgtttcc ataactattt gctcgcgttt tttactcagg aagaaaatgc
     1921 caaatagcaa catcaggcag acaatacccg aaattgcgaa gaaaactgtc tggtagcctg
     1981 cgtggtcaaa gagtatccca gtcggcgttg aaagcagcac aatcccaagc gaactggcaa
     2041 tttgaaaacc aatcagaaag atcgtcgacg acaggcgctt atcaaagttt gccacgctgt
     2101 atttgaagac ggatatgaca caaagtggaa cctcaatggc atgtaacagc ttcactaatg
     2161 aaataatcca ggggttaacg aacagcgcgc aggaaaggat acgcaacgcc ataatcacaa
     2221 caccgataag taatgcattt tttggcccta cccgattcac aaagaacgga ataatcgcca
     2281 tgcacagcgc ttcgagtacc acctggaatg agttgagata accatacagg cgcgttccta
     2341 catcgtgtga ttcgaataaa cctgcataaa agacaggaaa aagttgttga tcaaaaatgt
     2401 tatagaaaga ccacgtccca acaataaata tgacgaaaac ccagaagttt cgatccttga
     2461 aaactgcgat aaaatcctct ttttttaccc ctcccgcatc cgccgctacg cactggtgat
     2521 ccttatcttt aaaacacatg ttgatcatca taaatacagc gccaaatagc gagaccaacc
     2581 agaagttgat atggggactg atactaaaaa atatgccggc aaagaacgcg ccaatagcat
     2641 agccaaaaga tccccaggcg cgcgctgttc catattcgaa atgaaaattt cgcgccattt
     2701 tttcggtgaa gctgtcaagc aaaccgcatc ccgccagata ccccaggcca aaaaagagcg
     2761 cccccagaat tagacctaca gaaaaattgc tttgcagtaa cggttcataa acgtaaatca
     2821 taaacggtcc ggtcaagacc aggatgaaac tcatacacca gatgagcggt ttcttcagac
     2881 cgagtttatc ctgaacgatg ccgtagaaca tcataaatag aatgctggta aactggttga
     2941 ccgaataaag tgtacctaat tccgtccctg tcaaccctag atgtcctttc agccaaatag
     3001 cgtataacga ccaccacagc gaccaggaaa taaaaaagag aaatgagtaa ctggatgcaa
     3061 aacgatagta cgcatttctg aatggaatat tcagtgccat aattacctgc ctgtcgttaa
     3121 aaaattcacg tcctatttag agataagagc gacttcgccg tttacttctc actattccag
     3181 ttcttgtcga catggcagcg ctgtcattgc ccctttcgcc gttactgcaa gcgctccgca
     3241 acgttgagcg agatcgataa ttcgtcgcat ttctctctca tctgtagata atcccgtaga
     3301 ggacagacct gtgagtaacc cggcaacgaa cgcatctccc gcccccgtgc tatcgacaca
     3361 atccacagac attccagcaa aatggtgaac ttgtcctcga taacagacca ccaccccttc
     3421 tgcaccttta gtcaccaaca gcatggcgat ctcatactct tttgccaggg cgcatatatc
     3481 ccgatcgttc tgtgtttttc cactgataag tcgccattct tcttccgaga gcttgacgac
     3541 atccgccagt tgtagcgcct gccgcaaaca caagcggagc aaatgctcgt cttgccatag
     3601 atcttcacga atattgggat cgaagctgac aaaacctccg gcatgccggc tctccgtcat
     3661 cgcagtaaat gcgctggtac gcgaaggctc ggcagacaac gcaattgaac agagatgtaa
     3721 ccattcgcca tgtcgccagc agggcaagtc tgtcgtctct aaaaaaagat cggcactggg
     3781 gcggaccata aacgtaaatg aacgttcccc ttgatcgttc agatcgacaa gcaccgtgga
     3841 tgtccggtgc cattcatctt gcttcagata cgtgatatcg actccctcag ttagcagcgt
     3901 tctttgcatt aatgcaccaa aaggatcatc ccccacccga cctataaacc cacttgttcc
     3961 gcctaatctg gcgattccca ccgcaacgtt agctggcgcg ccgccaggac aaggcagtag
     4021 ccgcccgtct gattctggca agagatctac gaccgcatcc cctaaaaccc atactttggc
     4081 tgacattttt ttcccttaaa ttcatctgac ttacgcatag tgataaacct ctttttcgca
     4141 aaatcgtcat ggatttacta aaacatgcat attcgatcac aaaatgtcat agttaacgtt
     4201 aacatttgtg atattcatcg catttatgaa agtaagggac tttattttta taaaagttaa
     4261 cgttaacaat tcaccaaatt tgcttaacca ggatgataaa aatgacgcaa tctcgattgc
     4321 atgcggcgca aaacgcacta gcaaaacttc acgagcgccg aggtaacact ttctatcccc
     4381 attttcacct cgcgcctcct gccgggtgga tgaacgatcc aaacggcctg atctggttta
     4441 acgatcgtta tcacgcgttt tatcaacatc acccaatgag tgaacactgg gggccaatgc
     4501 actggggaca tgccaccagc gacgatatga tccactggca gcatgagcct attgcgctag
     4561 cgccaggaga cgagaatgac aaagatgggt gtttttcagg tagtgctgtc gatgacaatg
     4621 gtgtcctctc acttatctac accggacacg tctggctcga tggtgcaggt aatgacgatg
     4681 caattcgcga agtacaatgt ctggctacca gtcgggatgg tattcatttc gagaaacagg
     4741 gtgtgatcct cactccacca gaaggaatca tgcacttccg cgatcctaaa gtgtggcgtg
     4801 aagccgacac atggtggatg gtagtcgggg cgaaagaccc aggcaacacg gggcagatcc
     4861 tgctttatcg cggcagttca ttacgtgaat ggactttcga tcgcgtactg gcccacgctg
     4921 atgcgggtga aagctatatg tgggaatgtc cggacttttt cagccttggc gatcagcatt
     4981 atctgatgtt ttccccgcag ggaatgaatg ccgagggata cagttaccga aatcgctttc
     5041 aaagtggcgt aatacccgga atgtggtcgc caggacgact ttttgcacaa tccgggcatt
     5101 ttactgaact tgataacggg catgactttt atgcaccaca aagctttgta gcgaaggatg
     5161 gtcggcgtat tgttatcggc tggatggata tgtgggaatc gccaatgccc tcaaaacgtg
     5221 aaggctgggc aggctgcatg acgctggcgc gcgagctatc agagagcaat ggcaaacttc
     5281 tacaacgccc ggtacacgaa gctgagtcgt tacgccagca gcatcaatct atctctcccc
     5341 gcacaatcag caataaatat gttttgcagg aaaacgcgca agcagttgag attcagttgc
     5401 agtgggcgct gaagaacagt gatgccgaac attacggatt acagctcggc actggaatgc
     5461 ggctgtatat tgataaccaa tctgagcgac ttgttttgtg gcggtattac ccacacgaga
     5521 atttagacgg ctaccgtagt attcccctcc cgcagcgtga cacgctcgcc ctaaggatat
     5581 ttatcgatac atcatccgtg gaagtattta ttaacgacgg ggaagcggtg atgagtagtc
     5641 gaatctatcc gcagccagaa gaacgggaac tgtcgcttta tgcctcccac ggagtggctg
     5701 tggtgcaaca tggagcactc tggcaactgg gttaacataa tatcaggtgg aacaacggat
     5761 caacagcggg caagggatcc gcgtcacact tcccccttca cgaccttcaa taatatgcaa
     5821 tgcagcttcc cgcccgataa tgtcatgtgg aagctgaatt gtggtcagcg gcggtaaaaa
     5881 cagatgcccg acgccaacca gattatcaaa gcccattacg gcgacatcct gcgggatacg
     5941 taccccctta gccaggagaa cctgataagc cacaaaggct gcgcgatcgt taccacatat
     6001 cagaacatca aaatctggtt tgcccgattt gaagtggtca ttgagtaaac ttgcgagatc
     6061 ggtgtagtga tcatcacctg ttgccatgtg aaattgtttc acctcagcca gatctcgtcc
     6121 agcatcacgc caggcctgct caaatccctg ccgacgatac cctgttgcca acgcactttc
     6181 cggtagccag aagcataacg gttgacgata gcccgccgcg agcaaatgct gtgttgattc
     6241 atattgtgca gtgtaatcat cagggatata actgggtaac gctgggtcat ccgccacaca
     6301 gttcgccaat acaatatttt caccatacag agactcaggt agcgtgatat gtcgcagccc
     6361 cattgtagta tagataatgc catccggacg gtgggcaagc agctgacgtg ccgcgcgggc
     6421 agcgtcatct tcagaaaaaa tattgattaa aaaactattc cagccgaact cgctggcggt
     6481 ttgctcaatg gcaagcagaa tatcaacaga gaaaggagtg gtagcagtgt cctgcgccag
     6541 cacggcgaga gtcgacggct tacgtccttg agcgcgcatc ttacgggcgg aaagatcagg
     6601 aacataattc agggtctgga ttgcctgcaa tacgcggtca cgcgttgcag gacgcacaga
     6661 ttctgcatta tgcatcaccc gggagactgt catcatcgac actcccgcca ggcgtgcgac
     6721 atcctttaat gaagccatac ccaagccgtt tgccgtaaaa cgggcactgt agcagaaaca
     6781 gacgtcactg gcgagatcca acgaaattct cgacgcccta tcacccgaca cggcaataca
     6841 ataaaaaata acaataattg ccggataatt gtacccaatt ccgcctctgt tctcgccaac
     6901 gagtgaccat ataggcaagt aagaaaaaca tgccaactgg catcgccaac ctggagaaga
     6961 aaatataaat atggatcatt gtgctgccgc tgtatcccga catcagcccg gaaattattg
     7021 cgattgctat cggttcaggc gcgattggct gcacgatcgt tacggactcg cttttctggc
     7081 tggtgaagca atattgcggc gctacgctca atgaaacatt taaatactat acgacagcga
     7141 catttatcgc ttcagtcgtc gctctggcgg gcacattcct gctgtcattt atcatctaag
     7201 cgcaaagaga cgtactatgg aaaacgctaa aatgaactcg ctcatcgccc agtatccgtt
     7261 ggtaaaggat ctggttgctc ttaaagaaac cacctggttt aatcctggca cgacctcatt
     7321 ggctgaaggt ttaccttatg ttggcctgac cgaacaggat gttcaggacg cccatgcgcg
     7381 cttatcccgt tttgcaccct atctggcaaa agcatttcct gaaactgctg ccactggggg
     7441 gattattgaa tcagaactgg ttgccattcc ggctatgcaa aaacggctgg aaaaggaata
     7501 tcaacaaccg atcagcgggc aactgttact gaaaaaagat agccatttgc ccatttccgg
     7561 ctccataaaa gcacgcggcg ggatttatga agtcctggca catgcagaaa aactggctct
     7621 ggaagcgggg ttgctgacgc ttgaagatga ctacagcaaa ctgctttctc cggagtttaa
     7681 acagttcttt agccagtaca gcattgctgt gggctcaacc ggaaatctgg ggttatcaat
     7741 cggcattatg agcgcccgca ttggctttaa ggtgacagtg catatgtctg ctgatgcccg
     7801 ggcatggaaa aaagcgaaac tgcgcagcca tggcgttacg gtcgtggaat atgagcaaga
     7861 ttatggtgtt gccgtcgagg aaggacgtaa agcagcgcag tctgacccga actgtttctt
     7921 tattgatgac gagaattccc gcacgttgtt ccttgggtat tccgtcgcag gccagcgtct
     7981 taaagcgcaa tttgccgagc aaggtcgtat tgtcgatgct gataaccctc tgtttgtcta
     8041 tctgccgtgt ggtgttggcg gtggtcctgg tggcgtcgca ttcggactta agctggcgtt
     8101 tggcgatcat gttcactgct tttttgccga accaacgcac tccccttgta tgttgttagg
     8161 cgtccataca ggattacacg atcagatttc tgttcaggat attggtatcg acaaccttac
     8221 cgcagcggat ggccttgcag ttggtcgcgc atcaggcttt gtcgggcggg caatggagcg
     8281 tctgctggat ggcttctata cccttagcga tcaaaccatg tatgacatgc ttggctggct
     8341 ggcgcaggaa gaaggtattc gtcttgaacc ttcggcactg gcgggtatgg ccggacctca
     8401 gcgcgtgtgt gcatcagtaa gttaccaaca gatgcacggt ttcagcgcag aacaactgcg
     8461 taatgccact catctggtgt gggcgacggg aggtggaatg gtgccggaag aagagatgga
     8521 gcaatatctg gcaaaaggcc gttaataacg tttcaacgca gcatcgcaat cctttccctg
     8581 ggtgagcgat gctgccgatg gcgcagactt aagatcccca gtcttacccg ctataacccc
     8641 ctgcttttac catcacccaa cgcctttcgc tgtaaacggc ggtttcgcaa accaaaccaa
     8701 aaccgtaagt aagataaaag caatagccgc catacggaaa atctcatttg cagaaataga
     8761 aagtgactgc tgggttattt cattattaat ttcattaaga actcctgaaa gcgaaccata
     8821 gtatttatcc ataatttgtg acgatgaatt aaacacgggg ttaaattgat cgatggtttc
     8881 tgtcaactga ctatggtgta acgattcgcg tcgtccccac agcgtcattg tcaacgacgt
     8941 accaactgat cctgacaagg tacgaaaaaa attactcata ctcgaggcat tggcaaattt
     9001 attatctggc aagcctgaaa acgaaatcgt tgttaagggt aaaaagaaac aggcaacggc
     9061 gaatccctga aaaaactgcg gcaaaatgat acctgtaaaa tcaatcgttg gcataaatgt
     9121 cacagaacgc cagtaatagc aaaccgcata catcaaaaaa ctgaatgtca ccaacacccg
     9181 catgtctatt ttgttgccat aacgtcctat caaaggtgaa attaatagtg gcatgatgcc
     9241 gatgggcgca taagcaagtc cggcccatat cgcattatac cccatcgttt cctggagtaa
     9301 ctgcggcata aggacgatcg ctccagagta aaataaatac gcgcatgtga tactcacaat
     9361 accaatggtg aagttacggg acttaaacaa actgagatca agaatcgggt tctctgaggt
     9421 cgactcccaa atgactaaag agatcagaaa aataactgat actactgtta atattattat
     9481 tgtactcgag ttgaaccaat ccagatcgcg ccctttatca agcataattt gcaagccacc
     9541 aacaccgagc actaacaggg tcagtcctgg tagattcatt ttgaccggtg aagtctcagt
     9601 ttctcttcct ttaagtaagg ttaagcataa tgtcaggacg ataatcccca tagggacatt
     9661 gattaaaaat atccaacccc agctaaagtt atcacaaata taaccgccca atatcggccc
     9721 acatatcgga gcgataatca cggtcattga ccataatgcc agagcaaatg ttcttttttc
     9781 tggtggataa ttccttaata ataaactctg tgacagtgga attaacggcc ctgccattaa
     9841 cccctgaacg actctaaaaa atatcagcac atcaagattg atcgataaac tacacattaa
     9901 ggaagacagc gaaaaaaaag tgactgaaag taaaaataac cttaattcgc ctattctttg
     9961 tgccagcctg ccggtaacag ggatcgcaat ggcatttgct acgccaaacg aggtgataac
    10021 ccaggtgcct tcgtctgttg atgctcccag aaagccagat attgtcggta ttgcgacgtt
    10081 agaaatagtg gaatccaaca tttgcataaa cgtcgctaat gacaatgcaa tagtgacgca
    10141 ccataacgtc ccaccggtta atggtgccgg agttgattta gtgattgcca ttaaagttgt
    10201 ccattatgcg aaataatgtt gctaatttct ttttctatcg gactggtatc gataactaaa
    10261 gccttactgg tataagccgg catggacgtc acggttgaag ccagctcagg catctcagca
    10321 atgtcttcgt tcttcgtatc aatagttgca gtcatcgata aaccaatgcg caaggggtgt
    10381 tccatgagtt cttttggatc aagagaaact tcaaccggta cacgctgaac gattttgatc
    10441 cagttccctg tcgcattttg tgcaggtaat aaggagaacg cattgccggt tcccatattg
    10501 atccctgtca cccggccatg aaacacaaca ttctcaccat aaagatcgct gataatattg
    10561 accgattgac caatccgtac atccgtgagt tgtgtttctt taaagttagc attaacccac
    10621 atttgacgtg ccggtacgac agccattaac gattgtccgg gactcactgt ttcgccgacc
    10681 tgaacacttc tctgggcaat atagccggta accggactct taatatccgt acgtttaagc
    10741 gccaaccagg cttctttggt tgcatccgca gcttcaacga cttgtggctg acggtttaat
    10801 ggtgtgttca ttactaaagc tttattcgct ttataagcct ggatagcggc attcaatgcc
    10861 gctttgctac ttattaacgt atctttggta tgctccagcg tttcttttga aataaccccc
    10921 tgcttcgcta aaggcacacg acggttataa tcttctaaag attgttgata ctgaatacgt
    10981 gctgaagcga cttcggcact gtattgttta tcctgtaagt acagtttatt cgtttgccga
    11041 acaatatttg ccagattatt tttagcttta ttgagtgcga tagtggcatc agttttatcc
    11101 agtgaaacta aaatgtcacc ctgtcgaacg tagttcgtat ctttatgatt aacgacagtg
    11161 acactacctg agacttgtgc agaaattgga tctgcattcc ccgtgacata ggcgtcatct
    11221 gtactaatca tgtcttctaa ttccattgac caataggcat aagcacctga aaacgcaata
    11281 aataaaacta tcgccaataa agaaaagtat tttcttctgt tagaatgttt tttatttgaa
//
LOCUS       DAZKIP010000133        11331 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZKIP010000133 DAZKIP010000000
VERSION     DAZKIP010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.134"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            111..245
                     /locus_tag="ACHOUK_003917"
     CDS             111..245
                     /locus_tag="ACHOUK_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFP9899267.1"
                     /translation="MIYYLGYDRFLLIFFTDYSHFMDRITLPSTDTARGKGKWKIITA
                     "
     gene            224..328
                     /gene="speFL"
                     /locus_tag="ACHOUK_003918"
     CDS             224..328
                     /gene="speFL"
                     /locus_tag="ACHOUK_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFP9899268.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            639..2837
                     /gene="speF"
                     /locus_tag="ACHOUK_003919"
     CDS             639..2837
                     /gene="speF"
                     /locus_tag="ACHOUK_003919"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFP9899269.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVSDVE
                     RGKLDEIDATGYDIPVFIATENEERIPAEYLSRISGVFEHGEARKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRADLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFIPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2834..4153
                     /gene="potE"
                     /locus_tag="ACHOUK_003920"
     CDS             2834..4153
                     /gene="potE"
                     /locus_tag="ACHOUK_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFP9899270.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSHVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4210..5850)
                     /gene="pgm"
                     /locus_tag="ACHOUK_003921"
     CDS             complement(4210..5850)
                     /gene="pgm"
                     /locus_tag="ACHOUK_003921"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFP9899271.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKAVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5876..6421)
                     /gene="seqA"
                     /locus_tag="ACHOUK_003922"
     CDS             complement(5876..6421)
                     /gene="seqA"
                     /locus_tag="ACHOUK_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFP9899272.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPIKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6606..7370
                     /gene="ybfF"
                     /locus_tag="ACHOUK_003923"
     CDS             6606..7370
                     /gene="ybfF"
                     /locus_tag="ACHOUK_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFP9899273.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIAHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFSAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7510..7803
                     /gene="ybfE"
                     /locus_tag="ACHOUK_003924"
     CDS             7510..7803
                     /gene="ybfE"
                     /locus_tag="ACHOUK_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFP9899274.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7943..8473
                     /gene="fldA"
                     /locus_tag="ACHOUK_003925"
     CDS             7943..8473
                     /gene="fldA"
                     /locus_tag="ACHOUK_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFP9899275.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8683..8769
                     /gene="uof"
                     /locus_tag="ACHOUK_003926"
     CDS             8683..8769
                     /gene="uof"
                     /locus_tag="ACHOUK_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFP9899276.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8762..9208
                     /gene="fur"
                     /locus_tag="ACHOUK_003927"
     CDS             8762..9208
                     /gene="fur"
                     /locus_tag="ACHOUK_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFP9899277.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9292..9618)
                     /gene="chiQ"
                     /locus_tag="ACHOUK_003928"
     CDS             complement(9292..9618)
                     /gene="chiQ"
                     /locus_tag="ACHOUK_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFP9899278.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9668..11073)
                     /gene="chiP"
                     /locus_tag="ACHOUK_003929"
                     /pseudo
     CDS             complement(9668..11073)
                     /gene="chiP"
                     /locus_tag="ACHOUK_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
BASE COUNT         2774 a         2790 c         2810 g         2957 t
ORIGIN      
        1 gcgccaaata ttattatttg gcaggtttaa tttcttaacg caaatataaa acacgaaatt
       61 acaacattta agtaacaaaa cagcatctag tatggcgcta ttcataatta atgatttatt
      121 atctggggta tgatcgtttt ttgttgatct tcttcacaga ttatagccat ttcatggata
      181 gaataactct accttcaact gacacagcaa gaggtaaagg taaatggaaa ataataaccg
      241 cttaatgcct catataaggc ggacaaccca tatcatgaag tttgcccatc gtaatagttt
      301 tgactttcat ttctttaatg cccgctagtc ttctgactaa agggcacccc agcgtacagg
      361 tctccctgac tttaagcatt acaggttaat acctgtatcc ctcggtgctc atatactgct
      421 aaccctttta aactctacat aattcgagtc gcagcatatg caacttgaag tatgacgagt
      481 atagccagtt accgggctgg tctgggttat tgcatctgca aaaagcaaac tactgattta
      541 tttatcagcg gtggagcttt gctttttttc cggcgtgatc gatttctcct ttgagaaatt
      601 gaggacctgc tattacctga aataaagaga tgaacaaaat gtcagaatta aaaattgccg
      661 ttagtcgttc ttgcccggat tgtttttcca ctcatcgtgc atgcgtgaat atagacgaaa
      721 gtaattatat tgacgttgcc gccattattt tatcagtcag tgatgttgaa cgtggaaaac
      781 tcgatgaaat agacgctact ggctatgaca ttcctgtttt tattgcaacg gaaaatgaag
      841 aacgtatacc agcagaatat ctttcacgta tttctggtgt cttcgaacat ggagaagcac
      901 gcaaagaatt ttatggtcgt cagttagaaa ccgctgccag ccattatgaa actcaactgc
      961 gcccaccttt ctttcgcgca ctggtcgatt atgtcaatca gggcaacagc gcatttgact
     1021 gccccggtca tcagggcggc gaatttttcc gtcgccatcc ggcggggaat cagtttgtgg
     1081 aatactttgg tgaggcgctg ttccgtgccg acttgtgcaa cgccgacgta gcgatgggcg
     1141 atctgctgat tcacgaaggc gcgccatgca ttgcacagca acatgcagca aaagtgttta
     1201 atgccgataa aacctacttc gttttaaatg gcacttcatc ttctaacaaa gtggttttaa
     1261 acgccctgct gacaccgggt gatctggtgc tatttgaccg caataaccat aagtctaacc
     1321 accacggggc cttgctacag gctggtgcaa caccggttta tctggaaaca gcacgtaacc
     1381 cgtatggttt tatcggtggt attgatgcac actgcttcga agaaagttat ctgcgtgaac
     1441 tgattacaga agttgcaccg caacgggcaa aagaggcgcg tccgttccgc ctcgctgtga
     1501 ttcagctagg cacctacgac ggtacgattt ataacgcccg ccaggtggtg gataaaattg
     1561 gtcatctgtg tgactacatc ctgtttgatt ccgcatgggt cggctatgaa cagtttattc
     1621 cgatgatggc tgactgttcg ccgctgttgc tggatcttaa tgagaacgat ccgggtattc
     1681 tggttacgca atccgtacat aaacagcagg ctggtttttc tcaaacttca caaatccata
     1741 aaaaagacag ccacatcaaa gggcaacagc gttatgtacc gcacaaacgc atgaacaacg
     1801 cctttatgat gcatgcctcc accagcccgt tctatccgct gtttgccgca ctggatatca
     1861 acgccaaaat gcatgaaggt gtcagcggtc gtaatatgtg gatggattgt gtggtaaatg
     1921 gcattaatgc ccgcaaactg atcctcgata actgtcagca tattcgtccg ttcatacctg
     1981 aactggtgga tggtaaaccc tggcagtcgt atgaaacagc gcaaattgcg gttgatctgc
     2041 gcttcttcca gtttgtacca ggtgagcact ggcattcttt tgaaggctat gcagagaatc
     2101 aatactttgt tgatccgtgc aaactgttgc tgacaacccc aggtattgat gcacgtaacg
     2161 gcgaatatga agcgttcggt gtacccgcga cgattcttgc taacttcctg cgcgaaaatg
     2221 gcgtagtgcc ggaaaaatgc gatcttaact ccatcctctt cctgctgact ccggcagaag
     2281 atatggccaa acttcagcaa cttgttgccc tgctggtacg cttcgaaaaa ctgctggaat
     2341 ctgatgcgcc gctggcggaa gtgctacctt ccatctacaa acagcatgaa gagcgctacg
     2401 ccggttatac ccttcgtcag ttgtgccagg aaatgcatga tttgtatgcc cgccacaacg
     2461 tgaaacaact gcaaaaagag atgttccgta aggaacactt cccacgcgtc agcatgaatc
     2521 cgcaagaagc taactacgcc tatttacgcg gtgaagtgga gctggttcgt ctgccggatg
     2581 cagaaggccg tatcgctgcc gaaggtgcgc ttccttatcc tccgggtgtg ctgtgtgttg
     2641 ttccgggtga aatctggggt ggtgctgttt tgcgttactt cagcgctctg gaagaaggga
     2701 tcaacctgct gccaggtttt gcaccggagc tgcagggtgt ctatatcgaa gaacatgatg
     2761 gtcgtaagca agtttggtgc tatgtcatca agcctcgtga tgcgcaaagc accctgctga
     2821 aaggggaaaa attatgagtc aggctaaatc gaacaaaatg ggcgtcgttc agttaaccat
     2881 actgacgatg gtcaacatga tgggctccgg tatcatcatg ctgccgacaa agcttgccga
     2941 agttgggaca atctcaatta tctcctggct ggtgacagcc gtaggctcaa tggcactggc
     3001 atgggcattc gcaaaatgcg gtatgttcag ccgtaaatca ggcggtatgg gcggttatgc
     3061 cgagtatgct ttcggtaaat ccggtaactt tatggcgaac tatacctacg gcgtctcact
     3121 gctgattgct aacgtcgcga ttgctatttc ggcggttggt tacggcaccg aactgctcgg
     3181 cgcgagtttg tcgccagtgc agattggtct tgcgaccatc ggggtgctgt ggatttgtac
     3241 cgtggctaac tttggtggtg cgcgcattac cgggcaaatc agtagcatta ctgtgtgggg
     3301 agtcattatt ccggtcgtcg gtctgtgcat tattggctgg ttctggttta gcccgacgct
     3361 gtacgttgat tcctggaatc cgcatcatgc accgttcttc agtgcggtag gttcttccat
     3421 cgctatgacg ctgtgggctt ttcttggtct ggagtctgcg tgtgcaaata ctgatgtagt
     3481 ggaaaacccg gaacgtaatg tgccaatcgc ggtactcggc ggtacgttag gtgcggcggt
     3541 gatttatatc gtctccacca acgtgattgc cgggattgtg ccaaatatgg agctggcaaa
     3601 ttcaacggca ccatttggtc tggccttcgc gcagatgttc acgccggaag tgggtaaagt
     3661 cattatggcg ctgatggtga tgtcctgctg cggttcgctg cttggctggc agttcaccat
     3721 tgcccaggtg tttaaatctt catctgatga aggctacttc cctaaaattt tctcccatgt
     3781 aaccaaagtg gatgcaccgg tgcagggaat gttgaccatt gtgattatcc agagcgggtt
     3841 ggcgctgatg acgattagcc cgtcgctaaa cagtcagttc aacgtgctgg ttaacctggc
     3901 cgtggtgacc aatatcattc cgtatattct gtcaatggcg gcattagtca ttattcagaa
     3961 ggtcgctaat gtgcccccct caaaagcgaa agttgcaaac tttgttgctt ttgttggcgc
     4021 gatgtacagc ttttatgcgc tgtactcatc cggggaagaa gccatgctgt acggttccat
     4081 cgtgaccttc cttggttgga cactgtatgg tctggtatca ccacgctttg aactgaaaaa
     4141 taaacacggt taaaaaagca aagtaaaaaa gggcgatctt gcgaccgccc tttttcttat
     4201 ttaatgtgtt tacgcgtttt tcagaacttc gctaacaatc tcaaccgctt ctttctcaat
     4261 ctgcttgcga tgttcttcac cgaggaagct ttcgcagtag atcttatatg cgtcttccgt
     4321 gcctgacgga cgcgcggcga accagccgtt gtcagtcatc actttcagac cgccaataga
     4381 agcaccgttg cccggagcag cagtcaggcg cgcggtgatc gggtcaccag ccagggtgct
     4441 ggcactcacc atttccggag acagcttaga cagcgccgct ttttgtgcgg aagtcgcagc
     4501 tgcctgcaaa cggttgtagc tcggcgcacc aaagcgtttt gccagttcgt tgtagtgttc
     4561 ctgcgggttc ttaccggtga cagcggtgat ttccgccgcc agcagacaca tgatgatgcc
     4621 gtctttgtcg gtggaccacg gcgtgccgtc gaaacgcagg aaggaagccc ctgcgctctc
     4681 ttcgccgcca aagccgaagc tgccgtcgaa cagaccatca acaaaccatt tgaaacctac
     4741 cgggacttct accagtttac ggcccaagtc gttgaccaca cggtcgatca tcgcagatga
     4801 aaccagcgtt ttaccgacgg caacagcttt gccccactgc ggacgatgct ggaacaggta
     4861 gttgattgcc accgccaggt agtggttcgg attcatcaaa cctgccggag tgacgatacc
     4921 gtgacggtca taatccgggt cgttagcaaa cgccagatcg aacttatcac gcagtgccag
     4981 caggcccgcc atcgcacact cggaggagca gtccatacgg atcgcgccgt ctttatcaag
     5041 gtgcataaag cggaaggttt gatcgacctg atcgttaacg atggtcaggt tgaggttgta
     5101 atactcgcca atacgcttcc agtattcgat accggaaccg cccagcggat caacgccaag
     5161 cgtcaggccc gctttctgaa tcgccgccat atcaacgata tcggccagcc cttccacaaa
     5221 cggctgcacc agatcctgct ctttcacatg accggatgcc atcgcttcgt cgagggagat
     5281 acgcttcacg cctttcaggc catcggccat cagtgcgttg gctctgtctt ccaccacttt
     5341 agtgacgttg gtatcagccg ggccaccatt tggcggattg tacttgatac caccatcttc
     5401 cggcgggtta tgggacggtg taataacgat accgtctgcc agcgggccac cttttttatt
     5461 gtgaaccagg atggcattgg aaacggcagg cgtcggggtg aagccattgt tttcctgcac
     5521 aataacatct acgccgttcg ctgccagcac ttccagaacg gaaataaatg caggttcgga
     5581 cagggcgtga gtatctttac ccacatagca agggccagtg atgccgtttt tcgcacgttc
     5641 ttcagcaatt gcctgagcga tcgccagaat gtgcggctcg ttaaagctgt ggcgcgctgc
     5701 actgccacgg tgaccggaag taccgaattt caccgcgtgc tccgcattcc ctgcttctgg
     5761 tttcagtaca taatattgcg ccgtcagttg ggcgacgtta atcaaatcac tctgttgtgc
     5821 aggttggcct gcacgattgt ggattgccat tgctttgtcc tttgtctgca acgttttaga
     5881 tagttccgca aaccttctca atcaattccg ccgggaattg catcgactgc atgatgtgtt
     5941 cgatcatgct gcatttacgg ccggtgttgg tgttggtgat cacccaatac ggcgtgcctg
     6001 gcacatgttt cggcttggtc tgattaccat ttttcagcag cgtttgttca tctgccgcaa
     6061 agtaaacgcg tgtacgaccg tgcaacgatt ccgttgcttc ggcaaacgcc tgggcgtcaa
     6121 gagaatatag tgtagacaac agcagcataa agcgattgac cgctcgcttt tgctctgcat
     6181 attcatccga aagcagaagt tcacgcattg cgcgaacctt gtctttaatc gttttgatcg
     6241 gcttcgcttc gacgatagca ggtgacgcaa cgcgaacctc tttcgtcacc ggagcagcag
     6301 gctgtgatgc ggcggaaaat ttcaacatac gccgtaaaat gtcggatgcg ctctcgccga
     6361 tatgcttagt gtggctggca atatagctgt agagttcatc atcaacttca atcgttttca
     6421 tcttaatcca gtgcagtgtc ttagctgaat acaagtcgcc aggagtatat gaataaatcc
     6481 cggcagcgga tagcgtcaag cctggcttgc agaaaaactc ggaataaacc ttatttgttg
     6541 cagccctgtg gcacaatggt caacataata ccctaacccg acaacgcgaa aaaaagaact
     6601 ttgccatgaa attgaatatc cgcgcgcaaa ctgcacaaaa ccagcacaat aattctccca
     6661 tcgttcttgt ccatggtctg tttggcagcc tcgacaacct tggcgtactg gctcgcgatc
     6721 tggtaaacga tcacaatatc atccaggttg atatgcgtaa ccacggtctt tcaccgagag
     6781 atccggtaat gaattacccg gcgatggcgc aggatcttgt tgatactctg gatgcacagc
     6841 agatcgacaa agcaacattt atcgctcact ccatgggcgg taaagcggta atggcactta
     6901 ctgcactggc ccccgatcgc atcgataaac tggtggcgat cgatatcgca ccggtcgact
     6961 atcacgtacg ccgtcatgat gagattttct cggctatcaa cgcggtcagt gaatcggacg
     7021 cacaaactcg ccagcaagca gcagcaataa tgcgccagca tcttaatgaa gaaggggtga
     7081 ttcagtttct gttgaaatct tttgttgacg gggagtggcg ctttaacgtg ccggtattgt
     7141 gggatcagta tccgcatatt gtgggttggg agaaaatccc ggcatgggat caccctgccc
     7201 tgtttatccc tggtggtaat tctccgtatg ttagcgagca gtaccgtgat gatttactgg
     7261 cacaatttcc acaggcacgg gcgcatgtga ttgcaggcgc gggtcactgg gtccatgctg
     7321 aaaaaccgga tgcggtatta cgcgctatcc gtcgctatct caatgattaa cgcaactgat
     7381 taaaaaccca gcgtccgcgt cgctttccgc gcgtgggcgt tggcgtgact catcgcgctg
     7441 atgtattatg gcgcgctatc catccgggca gaggcctggc tgattgtttc ccccgaagtc
     7501 accaagatca tggccaaaga acaaacggac cgtacgacat tagatctgtt cgcgcacgag
     7561 cgtcgaccgg gacgaccgaa aacaaatccg ctttcgcgcg atgaacagct gcgtattaat
     7621 aaacgcaacc aactaaaacg cgacaaagta cgtggcctaa agcgtgtcga actgaagctg
     7681 aacgcggaag ctgtcgaggc gctgaacgag ctggcggaat cgcgcaatat gagccgtagc
     7741 gaactgatcg aagagatgtt aatgcagcaa ctggcggcgc tgcgtagtca gggcatcgtt
     7801 taaatttcca ctttcatgta gcacagtgtg cagtcctgct cgtttgctga ttccgcaaga
     7861 ctgcctgttc tgctatgatt gcctttatcc gtgggcaatt ttccaccccc atttcaataa
     7921 gtttcaagag gttatttcac tcatggctat cactggcatc tttttcggca gcgacaccgg
     7981 taataccgaa aatatcgcaa aaatgattca aaaacagctt ggtaaagacg ttgccgatgt
     8041 ccatgacatt gcaaaaagca gcaaagaaga tctggaagct tatgacattc tgctgctggg
     8101 catcccaacc tggtattacg gcgaagcgca gtgtgactgg gatgacttct tcccgactct
     8161 cgaagagatt gatttcaatg gcaaactggt tgcgctgttt ggttgtggtg accaggaaga
     8221 ttacgccgaa tatttctgcg acgcattggg caccatccgc gacatcattg aaccgcgcgg
     8281 tgcaaccatc gttggtcact ggccaactgc gggctatcat ttcgaagcat caaaaggtct
     8341 ggcagatgac gaccactttg tcggtctggc tatcgacgaa gaccgtcagc cggaactgac
     8401 cgctgaacgt gtagaaaaat gggttaaaca gatttctgaa gagttgcatc tcgacgaaat
     8461 tctcaatgcc tgatgtgatg cggcgtagac tcatgtctac gccgtattaa tagataatgc
     8521 caatcaaaat aattgctaca aatttgtaac ttttgccgtt gtacctgtac aatgtcccgg
     8581 tgttcaagtg gccttgccgt tgtaaatgta agctgtgcca cgtttttatt aacaatattt
     8641 gccagggact tgtggttttc atttaggcgt ggcaattcta taatgatacg cattatctca
     8701 agagcaaatt ctgtcacttc ttctaatgaa gtgaaccgct tagtaacagg acagattccg
     8761 catgactgat aacaataccg ccctaaagaa agctggcctg aaagtaacgc ttcctcgttt
     8821 aaaaatcctg gaagttcttc aggagccgga caaccatcac gtcagtgcgg aagatttata
     8881 caaacgtctg atcgatatgg gtgaagaaat tggtctggct acggtatatc gcgtactgaa
     8941 ccagtttgac gacgctggta tcgtcacccg ccacaatttt gaaggcggta aatccgtatt
     9001 tgaactgaca cagcaacatc accacgatca cctgatctgc ctcgactgcg gcaaggttat
     9061 cgaatttagt gatgattcca tcgaagcgcg tcagcgtgaa attgccgcaa aacatggcat
     9121 tcgcctgact aaccacagtc tctatcttta cggtcactgc gccgaaggcg attgccgcga
     9181 agatgagcac gcgcacgaag gcaaataagc cagtctgaac gagaaaagcc aacctgcggg
     9241 ttggcttttt tatgcaaggg aaataagaaa agcgagagtt acagctctca cttatttgtt
     9301 attactgcga atttcctgcc agaccttatc acaatcggct ttcaccgcct gatcatttcc
     9361 ggtttgcgtt gcacgcagac actgctgata atccagtacg cggacacttt cctggtcggc
     9421 aaactgctga tgttgtttct ctttcttcag cacgtttaac acgctctggc aggcttcaat
     9481 tttttccggc gaaccttgtg cggtgttgat acaggcgctg tatgcctctt tcagacggct
     9541 gtcttcctgt ggcgcggttg attgcgcaca agctaccagc cccgatgcca tcatggcgat
     9601 gagaatcagt tttttcatag caaattcctc aaacgtgcgg caggcacaaa acctgccgcg
     9661 tcgggcatca gaagatggtg aatggtgcga ttaccataaa ttttacgtca cgctcatcct
     9721 ggaagatgtt gccgtaaccg ccgccccagc ttgggatgtc ggagtggttg tcgtattcgg
     9781 tgaagtgcag tttgaacatc gtgcctttgg cgcgaccgtc ctgaatggtg tagaccgcat
     9841 ccaggctgta tgcagactct tcaatagtcc ggtttttgtc gtagtacgca tccggattgc
     9901 tctgccaggt cgcaggttta gcatcccatg cgtaaacgta ggaagcgccg atggcgaagc
     9961 ctggaagatt ccagtttttc aggtcataca tcgcaccgaa gaagaccgct ttttcgccgt
    10021 tggcgttgaa gtcagaacgg ttatcccacc agatatccag gcgaccgttc gaggaagcgt
    10081 aggttggagt catacgttgc aggaagtatc cctgctgacc gtcagcttta acccaggtgc
    10141 cttcgaggcg caaatccact acgtcagccg cccggtaacc aaaggtcaac gcttgcagcc
    10201 aggcggtgcc gtcataaaga tcgttgacgc tgcgatcgtc aactttatcg cgcgtaccgt
    10261 agaactggta gctggtggtt aacgggctac cggcgatatc aaatttgtag ctggctttgg
    10321 caaagtattg atcgatatac ccttccgcct gaccaaatgc cgcttccagt acgaagttat
    10381 ttttgaagtc gtatttcgcc ccaagggagt gcagataatc aactttggtg gttttatcgt
    10441 tctgataaaa ctcatccatt tccagatgcc acggtgcttt gtattcgttg gtccacatgt
    10501 aggagaaact caacgcacca gcatcgccgt aatcaaaatt cgccccggcc tccgcaccct
    10561 gataagtacc cggcataaag ctccagtgcg gcgctaacag cgtttgacca gttggctgaa
    10621 tataacctgc cctcgccaca ccggaccgta tttaaatttg gccgctgctt tatacaggct
    10681 tataccgctt ttatcgccgg accagtcttc gtcataggct ttattacttt ttgaaaacgc
    10741 gatttcgttc gggtgagagc tgtcgccgtt ttccgccatt tcaatcgccg taaacgcggc
    10801 aatatcaagg ccgaacatat cagcagcata accggactga aaatcgaggt tggcgttcca
    10861 ggtggagtga gaaaggttgg ttttgtattt atcgccgtcg gtaacatctt tacggtcgcg
    10921 ttcacgctgc cagtaataga taccgccggt taaggttgaa tcgtcaatga atccttcggc
    10981 gcgagcctcc ggcattgcca taaagcccga cattgctgta acaccggcga tagccagcgc
    11041 cagcgtacta cgtttgccac taaacgtacg catgggttaa tcctctttga cgtataaatt
    11101 gctgcaccac aggtgaacag cgaaaaaaat aaaaattaaa aaagtagcgt tgagaaaagt
    11161 ctcgctgact acaggaacag actatttttc gcgacgcgaa ttatcaatct ctttctgtaa
    11221 ccagaatgta agaacatgag caaccgcaca tattttgatg atttttgtta caaggcttaa
    11281 gtttgcggtt gaaaggcttg cagaagaata aaagcgtgaa attccagcaa g
//
LOCUS       DAZKIP010000134        11327 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZKIP010000134 DAZKIP010000000
VERSION     DAZKIP010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.135"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(400..666)
                     /locus_tag="ACHOUK_003930"
     CDS             complement(400..666)
                     /locus_tag="ACHOUK_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFP9899279.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGQTEAIYCQQGSDWQCVVHYDAEASTRQ"
     gene            complement(663..1430)
                     /gene="yjiL"
                     /locus_tag="ACHOUK_003931"
     CDS             complement(663..1430)
                     /gene="yjiL"
                     /locus_tag="ACHOUK_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFP9899280.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDCITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLRMPVNTHPDAQFAGTIGAAVIGQRVRTRR"
     gene            complement(1440..2591)
                     /locus_tag="ACHOUK_003932"
     CDS             complement(1440..2591)
                     /locus_tag="ACHOUK_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFP9899281.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKVRGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKAVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQFNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARIRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLKMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            complement(2707..3978)
                     /locus_tag="ACHOUK_003933"
     CDS             complement(2707..3978)
                     /locus_tag="ACHOUK_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFP9899282.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQKKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMTARADAVKSYLKEDEAFLSELWGDLREWLKADINSEDSRVKERIARAGQWFGETLI
                     ADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKDLQF
                     IRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            complement(4019..5251)
                     /gene="mdtM"
                     /locus_tag="ACHOUK_003934"
     CDS             complement(4019..5251)
                     /gene="mdtM"
                     /locus_tag="ACHOUK_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HFP9899283.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRKPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEEQ"
     gene            5718..6500
                     /locus_tag="ACHOUK_003935"
     CDS             5718..6500
                     /locus_tag="ACHOUK_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006685483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFP9899284.1"
                     /translation="MTNFTTSTPHDALFKSFLMHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYNAAFPLVDI
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEERFNEFISELTSRMPQHRERIMTIAERIHNDDCRANS"
     gene            6475..6675
                     /locus_tag="ACHOUK_003936"
     CDS             6475..6675
                     /locus_tag="ACHOUK_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899285.1"
                     /translation="MTIAERIHNDGWLLGREEGEQRILRLLLQNGADPEWIQKITGLS
                     AEQMQALRQPLPERERYSWLKS"
     gene            complement(6918..8330)
                     /locus_tag="ACHOUK_003937"
     CDS             complement(6918..8330)
                     /locus_tag="ACHOUK_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFP9899286.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDLVLDMLVAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQVARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLM
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQIYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVTRQLCRMKIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYRAME"
     gene            8507..8671
                     /locus_tag="ACHOUK_003938"
     CDS             8507..8671
                     /locus_tag="ACHOUK_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HFP9899287.1"
                     /translation="MEFHENKAKTPFIRLVQLWQAVRRWRRQMQARRVLQQMSDERLR
                     DIGLRREDVE"
     gene            8768..9649
                     /locus_tag="ACHOUK_003939"
     CDS             8768..9649
                     /locus_tag="ACHOUK_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFP9899288.1"
                     /translation="MKATEARLLDFLKRSQQFVIPIYQRTYSWTEQQCRQLWDDIIRA
                     GKRDDISAHFIGSVVYIEQGLYQVSGISPLLVIDGQQRLTTAMLLIEALSRHLGEDEV
                     FDGFSAMKLRNYYLLNPYESGEKGFKLLLTETDKDSLLALIKQRPMPENYSHRIMENF
                     TFFDEQIAKLGDDLIPLCRGLAKLLIVDVALNRGQDNPQLIFESMNSTGKALSQADLV
                     RNFILMGLEPEHQTRLYEDHWRPMEVACGQQGYSEYFDSFMRHYLTVKTGRSLGQMKS
                     MRHLNSMPAARVLLKKA"
     gene            9697..10860
                     /locus_tag="ACHOUK_003940"
     CDS             9697..10860
                     /locus_tag="ACHOUK_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1524 domain-containing protein"
                     /protein_id="HFP9899289.1"
                     /translation="MALGKESDKSLATAFQDLRELKVDVAYPFLLALYHDYKNDDLSH
                     EDFLSIIRLIESYVFRRAVCAIPTNSLNKTFATFYKVINKEKYLESIQVHFLNLPSYR
                     RFPNDDEFKRELKVRDLYNFRSRSYWLRRLENDKRRERVEEFTIEHIMPQNENLSAKW
                     REELGSDWQRIHKELLHTLGNLTLTRYNSRYSDRPFAEKRDIEDGFKHSPLYLNIGLG
                     QCEKWDEAAIHARADRLAELAVQVWQAPSLPEEVLAVYRGQPENKTSYSLSDYPFLAD
                     GSHSRVLFDHLRDEIMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLRLSLNMQFH
                     ELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMESALV"
     gene            complement(10907..11248)
                     /gene="symE"
                     /locus_tag="ACHOUK_003941"
     CDS             complement(10907..11248)
                     /gene="symE"
                     /locus_tag="ACHOUK_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HFP9899290.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTVVDVKVMEGCIVLTAQPPAAAESELMQSLRQVCKLSARKQRQVQEF
                     IGVIAGKQKVA"
BASE COUNT         2955 a         2814 c         2854 g         2704 t
ORIGIN      
        1 ggttgttttt ttcatggcaa gcttttagtc ccgaatacat gccgagtgtt gggaaaatat
       61 ctttgtatat gttaaatagg gagcccatat ttttatcctt gaactgtgaa atattcttct
      121 aaatctaaaa taatcagggg gtggggaaag ataagctttg cacagaaact tatcttttaa
      181 cattacaaca cccggtaatc attttatttc tctatgcatg attatgggtt taagtataat
      241 tcaatgtata ttgtatttta gacgcgcaat aggaaggtgc tatcaaaaat cgctcatttt
      301 gtggatggga aatatttcac ggcaggcgag atatttacta caacttcctt catgcaggat
      361 tatgcgcagc caattatccg gttggtatat ttaaagatcc tattgcctgg ttgacgcttc
      421 tgcatcgtaa tgcaccacgc actgccagtc gctgccctgc tggcaatata tcgcttcggt
      481 ttgcccaggg atcacccatt gtatttcctc gccgggggga caggtcagcc aaatgcataa
      541 cccgcagccg ccgcgtaaat cacggggaat atcgctgacg cgaaaagtca tgcccgctgc
      601 ctgcaacgcc ttgcgggttt gtatgacgcc gaccgtggag tgaaataaaa ataaaaactc
      661 tttcatcggc gtgtcctcac tcgttgacca attaccgccg cgccaattgt gccagcaaat
      721 tgcgcatcag gatgggtatt taccggcatt cgcaggtgag attccagcat ccgggcaaac
      781 gtctggcaat gactaacgcc accagtaaac agaatcggcg cttcacagga gagacgagca
      841 atgaaattgg cactccgccg cgccatcgcg ttaatcactc cggcgagaat cgcttctggc
      901 gcgacgcccg ctgagcgcag gctgatcact tctgattcag caaacactgt gcacatactc
      961 gtgatggcgt gcggcgtgac attttcggta atgcagtcga gttgctcgac gctggtgcca
     1021 agcgtgcgcg agatcacctc caggaaacgc ccggtgcccg ccgcgcattt gtcattcatc
     1081 aggaaatcgc acaggttacc gtcatcatca agctgaatca ctttgctgtc ctgaccaccg
     1141 atgtcgatta ccgcgcgcgt tgctggcgca agaaaccgtg cgcccagccc gtgacaggag
     1201 atttccgtca cttgtttatc ggcaaaatcc accagttgcc gcccgtagcc ggtgagcgtc
     1261 agaaacggcg ttgtctctaa cccttcgcgc agagtttccc aggcttcagt aattgctgtt
     1321 gccggtcgaa agggtgttgg aacgaggaaa cggcgcgtaa tcacgccgtc tgcgagtaag
     1381 atccctttgg tggcggtaga accagaatca atgccaatcg aatatgtcac tgctactcct
     1441 tacagcatct caataaaggc cgcgacacgg gtactgagct gcccaacatc cgaggtggag
     1501 tagtctgttt caatagcgat ataaggaatg ttgtgctgct gacgcacatg acgtttaatt
     1561 gccagcgatt ccaccgcgta ggtatggcac gcctgcaaaa tcacatcaac tacgccatcg
     1621 acctgatatt cttccaccat ctggctgagc attttcaggc gctgatcgtt cggcgaaaca
     1681 caggagcagc caatcgccag atatttatcc gccagcgcgt cgtagacatc gcccgtttct
     1741 gccacgcatt gctcggtcgc tttcgccccg gtgcagtttt cataaccgac aacccagccg
     1801 ccattctctt caatcgcgcg caccactttc tctgctgcgc cgccaatcgg gcagccggtg
     1861 attaaaatgc gcggacgcgg gtccagtcgc tggccttctt cccactgctg acgaatgcgg
     1921 gcggtcatcg cgtccagttc attgatcaac gcctctttat cgaaccggaa ggttgcgccg
     1981 taaaccactt tcagaatgtc gctgccgcta agcgccggag gattgaactg cccaagatga
     2041 taaaaattgg ccagtgcgcg acgttcgcgg tttttcagcg caatggcatc gcgcagagca
     2101 tcttcgctaa tttcgtgccc aaaacgttct tccaccgctt tttgtaagcg cagcatctcg
     2161 gctttccata acgcacgcga ggcatcgtct ttaacgctgt ttggcagctg catcacatga
     2221 acgggcttaa actccgccat gtattcatac attttctttt tgccgtcgca ggtggtttca
     2281 ccgaccacca gatccgaaaa gtagaagtag gggcatttat cggttttgcc gaagccgtag
     2341 ctacttttaa tcagcgggca gaggttgcgc ggcagatctt tctccgcttc ttcaatggtt
     2401 tcatcagagg tggaacagag cgaaaccaca accgcaccgg ctgccatcgg gatctcttgc
     2461 ggcataaagg tgcagtaagt gccaaccagc ggaatgccgc gcaccttgag atccatgacg
     2521 gtgagaaagc ctttctggcg agcttcagag aactgatcga aaatggcggg tagatcggtg
     2581 acaagtgaca tgattttcct tccccgtacc acgggagata atgaaaagag gccacattat
     2641 taccattctt ttagtatggt ttatttgatc tctctcgcgg ttagccactt agtttttcat
     2701 ggatttctaa aaattgccga gggggaacaa ggccgggagc tgtgacagca aatataaaat
     2761 tagcccaata caaccgccaa ccagcgtacc gttgacacgg ataaactgca gatctttgcc
     2821 gatatttaac tctatttgcc gcgacatatc ccgggcatcc cagcttttta ccgtatcgct
     2881 gatatggcgc gtcaggaatg cagaaaactc aggcgcgacg cggtgcgcgg cttgttccag
     2941 gtgaccattt aacgacgccc gcaaggcatc atcggcaatt aacgtttcgc caaaccattg
     3001 acctgcccgc gcgatacgtt ctttcacacg agaatcttca ctgttgatat ccgctttcag
     3061 ccattcccgt aaatcccccc acaattcact gagaaaggct tcatcctctt tcagatagct
     3121 ttttacggca tcagctcgcg ctgtcatttc aggatcgttt ttcaacttgt cgatcagggc
     3181 aaaagtggcg cgatcaaatg catggcggat ctgatgcgca cgatcgcggc tgatatcatc
     3241 aagcaaagaa ttcaccgcgt cagaaaccaa ctccgcgcta tgttcgccca gccattcggt
     3301 ggggagaatt ttggctttca gtggatgctc actctccagc cagcgaacaa tctgctgggc
     3361 gataaacttg cgcgatttat cgcgctggag aagggcgatc aactgtgcga tcagcgtatc
     3421 cagcagcacc tgatgacgat cgtttttggt catactctcc agcatcaacg cactggtgcc
     3481 ggaaagatcg accttatcaa tcgcccgatg gaccgcgcgc ttaagcaggc gctgaatacg
     3541 tgcatcatcg gtcagttcaa gaaaaccact catgatctgc aacagatgct gaccaacgcg
     3601 gcgggcgttt tctggctggc taaaccagtt gccaatcagc aacgccggtt cgtgacgtcg
     3661 aatcaatgcc accagcgatt gggtatcgag aaattttttc tgcacgaact gaccgagatt
     3721 ttcgccaatc cggtctttat tacgcgggat aatcgccgta tgacgagaaa tgatcggaat
     3781 cggcacgcgg cgaaacagcg ccaccaccgc aaaccagtcc gccagcgcgc cgaccatcgc
     3841 cgcttcagca atcgccttca cgccgctcac ccaaaaattg ggcggcaaaa acagcgtaac
     3901 gacaaaggta gcagcggcga taagcagtaa agagagcgcc aacattttgg cgcgtctgag
     3961 ttcaatgagt ttattcataa cgttaagaat agagcctgac ggcaggatcg cgcaaaaatc
     4021 actgctcctc tgccagctcg gcagcctgat gttggcgcac gcgattaagc aatccggcca
     4081 gggtgaaaac gacgataacg cccgccacaa cggctaacag atgaaacggc aagcgaccgc
     4141 cgttaaacca cagccagcgg ccaatttcga ccgagacaga catcaccatc aggatcacca
     4201 tattcagcga tgcggagacg gtccctttcg gtaagttatt ggaaaacagc gtaaagcgga
     4261 ataaggtcgg gaagatcaaa ccaatcccga aagcatacag actggtgccc agcaccgacc
     4321 acagccagac gtgcggtgac agcagattgc cgataatcaa cagcgcgagg ccgaccagtt
     4381 gaatgggtac ggcacgccag ataaagcgcg gttcggtcgg atctttaacg aaacgcgcca
     4441 cgatggcatt cgcgacaatc accgcgccga acaccggaac ctgtgtccag gcgaactgcg
     4501 aggtcgttaa gccgcctgcg tcaatcagga tcaccggcga gacagccacc caactcatca
     4561 tggggatata gcttaaagag atggttgctg cgccaaagag gaacagccga ttgcaaaaga
     4621 cattacgaaa atcgcgcaag acgcttttgg cgctaaatgg aaccgcgccg cgcttcaccg
     4681 tctctggcat cgccagcagt aagccaacaa atgagataaa acccataacc gcaatgatgg
     4741 caaaaaggac tttccagtgc acaaagtgca tcagagctgc gccggaaagc gggccgataa
     4801 tcggcgcaat cagtacgatg gaggtgataa tcgccatcaa cttgatccct tttgtctggc
     4861 cgaacgcctc ctgcaccgtg acataaccaa cggtggcaat aaaacagata ctggtgccct
     4921 gaattgcacg cgcgataaga aactgtgtca tagacgttgt gaacattgtc gcagcgcagg
     4981 caagggtaaa tattagcgcc ccggtaatca gcactggctt gcggccaatc ctgtcggaaa
     5041 gcggccccag cagccactgc aacgccatgc ctccagcgag atagagactg acggcagccg
     5101 gagccagact gacatcggca ttaaaatcac ggaccacatt aatgatccca ggctggatca
     5161 ggtccgtcga aagataggcg gcaaagtcat acaaaatcaa cgccatcggg aaaaacagcg
     5221 tggcggcatg gcgggcaaaa aaacgtggca taacaaacaa ctccttgtcc ggagaacccc
     5281 aacggggaaa acgaaattca ataccgtcgg taatgacgaa aggtaggcga gaaggacaaa
     5341 tgtactgaat ttcatgtaat caaccacact gcctgtgaac acacagacag agaaatacct
     5401 tctgacttcg ggtcagaatg tataacgatt acaaatagtc atcagcacat tacacctctt
     5461 gaattgatgg gttgcataga aaatatacac cttaagtgta attaaaattt gcgattaatc
     5521 aaaagagcgc ggtggaaggg ggaatatctg ccgggtacag gacatgtaaa acgcgagtca
     5581 gtttccggaa aaatgtatcg tttgcagacg aacaaaaata gcctggaaag cgcctcggga
     5641 aacgagaaat tgccgggtga gaatggtttt gttagtcgct aaagtcgggc catcttatca
     5701 acaggtgacg gatcgccatg acaaacttca cgaccagcac gccgcatgac gcattattta
     5761 aatcttttct catgcaccct gacaccgcgc gggattttat ggagatccac ttacccaaag
     5821 atttacgtga actgtgcgat ctcgacagct taaaactgga atccgccagc tttgtcgatg
     5881 aaaaattgcg ggcgctacac tccgatattc tgtggtcggt aaagacccgg gaaggcgatg
     5941 gctatatcta tgtggtgatt gaacatcaga gccgtgagga tattcatatg gcctttcgcc
     6001 tgatgcgcta ttccatggca gtgatgcagc gccatataga gcatgataaa cgccggccac
     6061 taccgctggt catcccgatg ctgttttatc acggtagccg tagtccttat ccctggtctt
     6121 tgtgctggtt ggacgaattt gccgacccga ctactgcacg gaagctttat aacgccgcgt
     6181 tcccgctggt ggatatcact gtcgtgccgg acgacgagat tgtgcagcac cgcagagtcg
     6241 ccctgttgga gttgatccaa aagcatattc gccagcgcga tctgatgggg cttatcgatc
     6301 aactggtagt attactggtt acagagtgtg ctaatgacag ccagataact gcgctgttaa
     6361 attacatttt actgactggc gatgaagagc gttttaatga gtttatcagt gaactaacca
     6421 gtcgaatgcc tcaacacaga gagcgaataa tgacgattgc agagcgaatt cataatgacg
     6481 attgcagagc gaattcataa tgatggatgg ctgttgggaa gggaagaagg ggaacagcgc
     6541 attcttcgat tgttgttgca gaatggggca gatcctgaat ggatacagaa gattaccgga
     6601 ctttcggcag agcaaatgca ggcattaagg cagcccttgc ctgagcgtga gcgctattca
     6661 tggctcaaga gctaaccaga gacggatgac aaacgcaaag cagcctgatg tgctacgctt
     6721 atcaggtcta caaagcccat tcaatatatt gaactttaaa gattttgtag acctggtaag
     6781 gcgttcacat ggcatccggc atgaacaaag cgcactttac ctaagccatg ccggaacgtg
     6841 gctatgatcc tgcaaagata aaacgagtca caggcgattc gtgagaataa atcaggtgct
     6901 tcgccactaa ttctaattta ttccattgcc cgatacacgg cctcgccaat ttgcttcagc
     6961 gcttcgcgat aagtttcgct gagtggcaaa gcacagttga tgcgcaggca gttacggtat
     7021 ttgccggaag ctgagaaaat cgagcctgcc gccacctgga ttttcatgcg gcacagctgc
     7081 cgcgtgacgc agaccatatc gacctgttca ggcaattcta tccacagtaa aaatccgcct
     7141 ttcgggcgcg taatacagat ttcgcaggga aaatattccc gtatccagca ggtataaagc
     7201 gccaaattgc gctgatagat ctgccgcatc cgccggatat ggcgatgata gtgaccttcc
     7261 agcacaaacg ttgccgccgc catttgcgtg gacggcacat taaagctgct gatggcgtat
     7321 ttcatatgca tcagtttatc gtgataacgc cccggtgcga cccaacccac gcgcaggcct
     7381 ggagcaatac ttttactgaa cgagctgcac aacagcactc gcccgtcgat atcccaggaa
     7441 tgaatggtcc gcgggcgcgg atactccgtt gccagttcgc cgtagacatc atcttcaaaa
     7501 atcacaatat catgacgctg agcgagagag agaacggccc gtttgcgcgc gtccggcata
     7561 ataaatccca gcggattatt acagtttggc accagaatga tgcctttaat cggccactgt
     7621 tccagcgcca gttccagcgc ttcaacgctg atgcctgttt ctggatcggt tgggatttca
     7681 atcactttca cgcccatgcc gcgcagcatc tgcattgaac cgtaataaca gggggattcg
     7741 accgcgacaa tatcgcccgg tttacacacc gccattaacg ctagcgacat cgagttatgg
     7801 cagccgctgg tgatgatgat gtcatcggca gtgaccaccg agccgctgtc gagcatcagg
     7861 cgggcaacct gctcgcgcaa tacccgctga ccggctagca agtcataacc gagaacggtt
     7921 tgcagattat gctgcaccac ccggcttagc tcccgccaca gcggtttcag gctgggcgtt
     7981 tcgacatccg gcgtgctttt gcttaacgga acaatggaac tgtcgctatg cgccaccagc
     8041 atatccagca ccagatccca ctgggtaatt tccactgggc gctgcaccgg acgcgtcatc
     8101 ggcggcactg gcggctgggc tttacgttgt gcgacaaaat aacccgaacg cggctgcgga
     8161 gtgatgagct tcatcgtctc cagcgtctgg tacgcctgct gcacggtgct gatgctgacg
     8221 ccgtgctcct gacttaagct gcgcaccgac ggcaatttct ccccgtgacg atacagccct
     8281 tgctcaatcc gctcggcaag tagagtcgcc agatgttgat aacgcgtcat gctgtattcc
     8341 ttatttggac catacagaaa gaaaaaccgg tacagatgag gctaaaaaac cggatgcaga
     8401 tgctgtttta acctgtctgt atgttaaaca aaactaatat ttgcatctgt attgtgaact
     8461 gccagaagac ggatgatgaa gcctctgaaa tatgaggagg cagcagatgg aatttcacga
     8521 aaacaaagcc aaaacgccgt ttatcagact tgtgcaactc tggcaggcag tgaggcgttg
     8581 gcggcggcaa atgcaggccc gacgggtgtt acagcagatg agtgatgaac ggttgaggga
     8641 tattgggtta cgcagggagg atgtggagtg aggggtatgt tcgtcgcgga taaacaggat
     8701 ttctctataa cctccataag gaagtggtta ttattgcgaa atcatcgttt cacggaaaga
     8761 cgtcagaatg aaagctacag aagctcgcct actcgatttt ttaaaacgtt cgcagcagtt
     8821 tgttattccc atttatcaac ggacttattc atggacagaa caacaatgtc ggcaactttg
     8881 ggacgacatc attcgtgcag gaaagcgtga cgatatatca gcgcatttta tcggttcggt
     8941 tgtttatata gagcagggat tgtatcaggt ttctggtatt tctccgttgc tggtcattga
     9001 tggtcagcag cggctgacga ccgcaatgtt gctgattgag gctttatcgc gccatcttgg
     9061 cgaagacgaa gtttttgatg gcttttcggc aatgaaattg cgtaattatt atttgctcaa
     9121 tccttatgag tccggcgaga aaggttttaa attactgctg accgagactg ataaagacag
     9181 tttactggcg ttaataaaac aaagaccaat gccagaaaac tattcccatc gaataatgga
     9241 aaactttact ttctttgatg aacaaattgc caaactcggt gatgacttga tccccttatg
     9301 tcgtgggtta gcaaagttat taattgtcga tgtggcgctt aatcgtggtc aggataatcc
     9361 gcaactgatt tttgaaagta tgaactccac cggtaaggcg ttaagtcagg ccgatctggt
     9421 gcgcaatttt attctgatgg gcctcgaacc agagcatcaa acccggttgt atgaagatca
     9481 ctggcgtcca atggaagtcg cttgtggtca gcaaggttac agcgaatatt ttgacagttt
     9541 tatgcgtcat tatctgacgg taaaaacggg gagatccctc ggacagatga agtctatgag
     9601 gcatttaaac tccatgcccg cagccagagt gttgctgaaa aaggcgtaga tcggctggtt
     9661 gaagatattc atatctacgc ggagtattac tgtgcaatgg cattgggaaa agaaagtgac
     9721 aaatcgcttg ctacggcttt tcaggatttg cgcgagttaa aggttgatgt ggcgtatcct
     9781 ttcttactgg cgctttatca tgactataaa aatgacgatt tgtctcacga agatttcctg
     9841 agcataattc gtttaattga atcttatgtt ttccgccgtg cagtatgtgc gattccgaca
     9901 aattctctta acaagacgtt tgctactttt tataaggtca ttaataaaga aaaatatctg
     9961 gaaagtattc aggtacattt tttgaatcta ccttcatatc gtcgtttccc caacgatgat
    10021 gaatttaaac gggaattaaa agttcgcgat ctctataact tccgtagtcg tagctactgg
    10081 ttacgacgac tggaaaacga taaacgcaga gagcgcgtgg aagagtttac gattgaacat
    10141 attatgccgc agaacgaaaa tttgtcggct aaatggcgcg aagagctggg aagtgactgg
    10201 cagcgtattc ataaagaact gttgcatacg ttggggaatc tcactttaac gcgctataac
    10261 tcccgttaca gtgacagacc tttcgcagaa aaacgcgata ttgaagacgg ctttaagcat
    10321 agcccgctct atttgaatat cggtcttgga cagtgcgaaa aatgggatga agctgccatt
    10381 cacgcccgag ccgatcgtct ggcggagctt gcggttcagg tctggcaagc gccttctctt
    10441 cctgaagagg ttttagctgt ttatcgggga cagcctgaga acaaaaccag ttacagcctg
    10501 agtgattatc cttttcttgc tgatggttcg catagccggg tgttattcga tcatcttcgc
    10561 gatgaaatta tgcgcctgga cgcagggatc acgcaggaag tattgaagct ctatattgcg
    10621 tttaaagcgg aaacgaattt tgttgatgtt gtgccgcaaa aaagccgact gcgattgtcg
    10681 cttaatatgc agtttcatga actggtcgat ccgaaaggta ttgccaaaga tgtgacaaat
    10741 gttgggcgat ggggcaatgg tgatgtggaa attggtttca gtgacctcgc acaacttcct
    10801 tacattatgg ggttaattcg tcaggcattt gaaaaacaga tggagagcgc gttggtataa
    10861 cgcagcgaaa aatgcccggt agtcctggcg ctaccgggtc aatgtatcag gcaactttct
    10921 gtttacccgc aatcacccca ataaactcct gcacctgcct ttgtttacgc gccgacagct
    10981 tgcacacctg gcgcagcgac tgcatcagtt cgctctcggc ggcggcgggt ggttgggcgg
    11041 tgaggacaat acagccttcc atcactttga catctaccac cgtgccggtg gcaaaaccgg
    11101 cggcttccag ccactgacct ttcagggtga tggcgggaat acggctgtaa tccgggtaac
    11161 gactcgcata actcacggtt aattgacggt tatttgccgg ggagacttct gcttcgaacg
    11221 gttgtgcaat agaatgcgta tcagtcataa ctgctattcc ctataaatag tgattgtgat
    11281 tagcggtgcg ggtgtgttgg cgcacatccg caccgcgcta aatacct
//
LOCUS       DAZKIP010000135        10976 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZKIP010000135 DAZKIP010000000
VERSION     DAZKIP010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.136"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            142..903
                     /gene="adiY"
                     /locus_tag="ACHOUK_003942"
     CDS             142..903
                     /gene="adiY"
                     /locus_tag="ACHOUK_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFP9899291.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            1040..2377
                     /gene="adiC"
                     /locus_tag="ACHOUK_003943"
     CDS             1040..2377
                     /gene="adiC"
                     /locus_tag="ACHOUK_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFP9899292.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2481..4124
                     /gene="eptA"
                     /locus_tag="ACHOUK_003944"
     CDS             2481..4124
                     /gene="eptA"
                     /locus_tag="ACHOUK_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFP9899293.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4121..4789
                     /gene="basR"
                     /locus_tag="ACHOUK_003945"
     CDS             4121..4789
                     /gene="basR"
                     /locus_tag="ACHOUK_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFP9899294.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4790..5890
                     /gene="pmrB"
                     /locus_tag="ACHOUK_003946"
     CDS             4790..5890
                     /gene="pmrB"
                     /locus_tag="ACHOUK_003946"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFP9899295.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5866..5955)
                     /gene="pmrR"
                     /locus_tag="ACHOUK_003947"
     CDS             complement(5866..5955)
                     /gene="pmrR"
                     /locus_tag="ACHOUK_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFP9899296.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(6067..7569)
                     /gene="proP"
                     /locus_tag="ACHOUK_003948"
     CDS             complement(6067..7569)
                     /gene="proP"
                     /locus_tag="ACHOUK_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFP9899297.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAVA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7833..8711)
                     /gene="rdcB"
                     /locus_tag="ACHOUK_003949"
     CDS             complement(7833..8711)
                     /gene="rdcB"
                     /locus_tag="ACHOUK_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFP9899298.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMREEIFSVNLV"
     gene            complement(8708..10936)
                     /gene="crfC"
                     /locus_tag="ACHOUK_003950"
     CDS             complement(8708..10936)
                     /gene="crfC"
                     /locus_tag="ACHOUK_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFP9899299.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQKLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPPPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQMLLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQIEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALKVQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2751 a         2867 c         2692 g         2666 t
ORIGIN      
        1 atttttataa aaatttgtaa tattagtaaa actcgttatt tttatgcatg tttatattca
       61 tcatacaatt atataaccat ttcccggtat cgctttgctt tagcgagaac tggtctttta
      121 ttcgcactca ggagtacatg tatgaggatt tgcagcgacc aaccttgtat tgttttattg
      181 actgaaaaag atgtctggat aagggttaat gggaaagaac ctattagcct taaagctaac
      241 catatggcgt tattaaattg tgaaaataat attatcgacg tctcctctct taacaacact
      301 ttggttgctc atattagtca cgacatcatc aaagattacc tccggtttct gaataaagat
      361 ctctcgcaaa taccagtatg gcaacgtagc gctacgccca ttctcaccct gccatgcctg
      421 acaccagacg tctttcgcgt tgccgcgcaa cacagcatga tgcccgcaga aactgagtca
      481 gaaaaggaac gaacacgcgc attattattc acggtgctat cccgttttct cgacagtaaa
      541 aaattccttt cattaatgat gtatatgtta cgtaattgtg taagtgacag cgtttatcaa
      601 attattgaaa gcgatattca caaagactgg aatcttagta tggtagccag ttgtttatgt
      661 cttagcccaa gtctgttaaa gaaaaagctg aaaagcgaaa acaccagtta tagccaaata
      721 atcaccacct gccgcatgcg ttatgccgta aatgaattaa tgatggacgg taaaaatatc
      781 tctcaggtat cacagtcctg cggctacaac agtacgtcgt actttatttc tgtctttaaa
      841 gacttctacg gtatgacacc gctgcattat gttagtcagc acagagaacg cactgtcgcc
      901 tgatttttaa ccttaacgaa gagctatatt aataacggca tcagcgataa cccggtcgat
      961 aataattcaa ctatcgaatg caggcgtatg atatgacgta atttattgtc acgaagctcg
     1021 ccttcgcagg agtttaatta tgtcttcgga tgctgatgct cacaaagtgg gcttaatccc
     1081 cgtcaccctg atggtgtcgg ggaatattat ggggtcaggt gtttttctgt tacctgcaaa
     1141 cctggcctcc accggcggga ttgccattta tggatggttg gtgacgatta tcggtgcgct
     1201 ggggctctcg atggtatacg ccaaaatgtc gttcctcgac ccaagtcctg gtggttctta
     1261 cgcttacgcc cgccgctgct ttggcccgtt tctcggttat caaaccaacg tcctctactg
     1321 gctggcctgc tggatcggca atatcgccat ggtggtcatt ggcgtaggat atttaagtta
     1381 cttcttcccg attctgaaag atccattggt attaaccatc acctgcgtcg tggtgctgtg
     1441 gatcttcgtc ctgctgaaca ttgtcggtcc gaaaatgatc acccgtgtgc aggccgttgc
     1501 caccgtgctg gcgctgatcc ccatcgtcgg gattgccgta tttggctggt tctggtttcg
     1561 tggtgaaacc tatatggcgg catggaacgt cagcggcctg ggcaccttcg gtgcaattca
     1621 aagtaccctt aacgttacgc tgtggtcgtt catcggtgtg gaaagtgcct ccgttgccgc
     1681 aggtgtggtg aaaaacccga aacgcaacgt ccctatagcc accattggtg gggtattgat
     1741 tgccgccgtt tgctatgtgc tctccaccac cgcgattatg gggatgatcc ctaatgccgc
     1801 actgcgcgtt tctgcctcgc cattcggtga tgccgcacgg atggcgttgg gtgacaccgc
     1861 cggggccatc gtttccttct gcgcagctgc gggttgctta ggttcactgg gcggctggac
     1921 attgctggcg ggtcaaacgg cgaaagcggc tgccgatgac ggactgttcc caccgatttt
     1981 tgcccgtgta aataaagcgg gtacgccagt ggcggggctg attatcgtcg gtattttgat
     2041 gaccatcttc cagctcagca gcatttcacc aaacgcgacc aaagaattcg gtctggtttc
     2101 ttccgtgtcg gtcatcttta cactggtgcc atatctttac acctgtgcgg cgttactgct
     2161 gctcggacac ggtcactttg gtaaagcacg cccggcatat ctggcggtta ctaccattgc
     2221 cttcctctac tgcatctggg ccgtggtggg gtccggggcg aaagaggtta tgtggtcatt
     2281 tgtcaccctg atggtcatca ccgccatgta tgcgctgaat tacaaccggc tgcataaaaa
     2341 cccgtatccc ttagatgcac caataagcaa agattaattc cccttaatcc agcaaagata
     2401 aaagccaacc ttaagaactt aaggttggct taattttgct ttgcgagcat atgcgcactt
     2461 tgttcgatgg aaacaccgtg atgttgaagc gcctactaaa aagaccctct ttgaatttac
     2521 tcgcctggct attgttggcc gctttttata tctctatctg cctgaatatt gcctttttta
     2581 aacaggtgtt gcaggcgctg ccgctggact cgctgcataa cgtactggtt ttcttgtcga
     2641 tgccggtcgt agccttcagc gtgattaata ttgtcctgac actaagctct ttcttatggc
     2701 ttaatcgacc gctggcctgc ctgtttattc tggttggcgc ggctgcacaa tatttcataa
     2761 tgacttacgg catcgtcatc gaccgctcga tgattgccaa tattattgat accactccgg
     2821 cagaaagcta tgcgctgatg acaccgcaaa tgttattaac gctgggattc agcggcgtgc
     2881 ttgctgcgct gattgcctgc tggattaaaa tcaaacctgc cacctcgcgt ctgcgcagtg
     2941 ttcttttccg tggagccaat attctggttt ctgtactgct gattttgctg gtcgccgcac
     3001 tattttataa agactacgcc tcgttgttcc gcaacaacaa agagctggtg aagtccttaa
     3061 gcccctctaa cagcattgtt gccagctggt catggtactc ccatcagcga ctggcaaatc
     3121 tgccgctggt gcgaattggt gaagacgcgc accgcaaccc attaatgcag aacgaaaaac
     3181 gtaaaaattt gaccatcctg attgtcggcg aaacctcgcg ggcggagaac ttctccctca
     3241 acggctaccc gcgtgaaact aacccgcggt tggcgaaaga taacgtggtc tatttcccta
     3301 ataccgcatc ttgcggcacg gcaacggcag tttcagtacc gtgcatgttc tcggatatgc
     3361 cgcgagagca ctacaaagaa gagctggcac agcaccagga aggcgtgctg gatatcattc
     3421 agcgagcggg catcaacgtg ctgtggaatg acaacgatgg cggctgtaaa ggtgcctgcg
     3481 accgcgtgcc tcaccagaac gtcaccgcgc tgaatctgcc tgatcagtgc atcaacggcg
     3541 aatgctatga cgaagtgctg ttccacgggc tggaagagta catcaataac ctgcagggtg
     3601 atggcgtgat tgtcttacac accatcggca gccacggtcc gacctattac aaccgctatc
     3661 cgccgcagtt caggaaattt accccaacct gcgacaccaa tgagatccag acctgtacca
     3721 aagagcaact ggtgaacact tacgacaaca cgctggttta cgtcgactat attgttgata
     3781 aagcgattaa tctgctgaaa gaacatcagg ataaatttac caccagcctg gtttatcttt
     3841 ctgaccacgg tgaatcgtta ggtgaaaatg gcatctatct gcacggtctg ccttatgcca
     3901 tcgccccgga tagccaaaaa caggtgccga tgctgctgtg gctgtcggag gattatcaaa
     3961 aacggtatca ggttgaccag aactgcctgc aaaaacaggc gcaaacgcaa cactattcac
     4021 aagacaattt attctccacg ctattgggat taactggcgt tgagacgaag tattaccagg
     4081 ctgcggatga tattctgcaa acttgcagga gagtgagtga atgaaaattc tgattgttga
     4141 agacgatacg ctgttattgc agggactgat tctggcggcg caaaccgaag gctacgcgtg
     4201 cgatggcgtg acaaccgcgc ggatggcgga acaaagcctt gaggcaggtc attacagcct
     4261 ggtggtactg gatttagggt tacccgacga agatggactg cattttctcg cccgtatccg
     4321 gcagaaaaaa tataccctgc cggtactgat cctcaccgct cgcgatacgc tgaccgacaa
     4381 aatcgccggg ctggatgtcg gtgccgacga ctatctggtg aagccttttg cgctggaaga
     4441 gttacatgcc cgtatccgcg ccctgctacg acgccataat aatcagggcg aaagtgagct
     4501 gattgttggc aatctgacgc tgaacatggg tcgccgtcag gtatggatgg gcggtgaaga
     4561 gttgattctg acgcccaaag aatatgctct gctgtcacgg ttaatgctca aagcaggcag
     4621 tccggtgcat cgggaaattc tctacaacga catctataac tgggacaatg aaccctcgac
     4681 caacaccctg gaagtgcata tccacaatct gcgcgacaaa gtgggcaaag cccgtatccg
     4741 caccgtgcgc ggctttggct atatgcttgt cgcgaatgag gaaaactaat tgaatctgat
     4801 gcgttttctg cgccgaccaa tatcgctgcg ccaacggctg atattgacca tcggggccat
     4861 tttgttggtg tttgagctga tcagcgtctt ctggctatgg catgaaagta ccgagcagat
     4921 tcagctgttt gagcaggcgc tacgcgacaa tcgcaacaac gatcgccaca tcatgcgtga
     4981 gatccgcgag gcggtcgcca gcctgattgt ccccggcgtc tttatggtca gcctgacgct
     5041 atttatctgc tatcaggcgg tacgccgcat cacccgcccg ctggcggagc tgcaaaaaga
     5101 gctggaagcg cgcaccgccg acaacttaac gcccattgcc attcacagcg ccaccctcga
     5161 aatcgaagcg gtggtttcgg cgttgaacga tctggtcagt cgcctgacca gcacgctgga
     5221 taacgaaagg ttatttaccg ctgacgtcgc gcacgaactg cgaacgccac tggcgggggt
     5281 gcgtttgcat ctggaactgc tggcgaaaac gcatcacatt gatgtagcac cgttagtggc
     5341 acggcttgat cagatgatgg agagcgtctc ccagctgctg caactggcgc gtgccggaca
     5401 gtcattttct tccggtaatt atcaacatgt aaaactgctg gaagatgtca ttctcccctc
     5461 gtatgacgaa ctcagtacca tgctcgacca gcgacagcaa accctgctat tgccagagag
     5521 cgccgccgac atcaccgttc agggtgatgc aaccctgctg cggatgttat tgcgaaacct
     5581 ggtagaaaac gcccatcgtt acagcccgca aggcagcaac attatgatta agctgcaaga
     5641 agatggcgga gcggtcatgg ccgttgaaga tgaaggaccg ggtattgatg agagtaaatg
     5701 cggggagttg agtaaagcgt ttgtacgtat ggacagccgt tatggcggga ttggtctggg
     5761 gttaagtatt gtcagccgca ttacacagtt gcatcacggg cagtttttcc tgcaaaaccg
     5821 gcaagagact tccggcacgc gggcctgggt acggctgaag aaagatcaga acgtggcaaa
     5881 ccagatataa agaaagctgc tgaccaccag cacgctgaac acggtagtta aactttcata
     5941 aacacggttt ttcattgcac tctcctccgt taacctggag gagagtatgc gcgtcagaga
     6001 ttaagtgaac cttaagagtt caattggcct gataagacag cgttacatca ggccatccgt
     6061 ttcagcttat tcatcaattc gcggatgttg ctgcaccagg cgggtacgtt tcgcctgcaa
     6121 atcggcaatc tcgtggtcaa tatcatcgat tttctgctcg atattatcgt aatgctcgac
     6181 gagaatttcc ttcgcttcct gtatatctga cgccgccggt gttgcacctt tcaacggacg
     6241 atttgccgtc tctttcatgg ttacgccggt gattaaacca accaccgcca ctaccatcag
     6301 gtaataggct ggcatcatca gattctgcga gctttcgacc agccaggccg ccagtgttgg
     6361 cgtcagaccg gcaaccagca ccgaaatatt aaatgccacc gccagcgcgc tgtaacggat
     6421 atgcgtcggg aacatcgctg gcaaggtaga agccataacg cccgtaaagc agttaaggat
     6481 caccgccagc atcagtaacc cggcaaaaat caggccgatg acgttactgt taatcagaat
     6541 aaacgccggg atcgccaaca caaacagtgc aacactacca agtagcacaa acggacgacg
     6601 gccaaaacgg tcactcagca agcccatcac cggctggaca aacagcatac cgatcataat
     6661 ggcgataata atcagcaccc cgtggtcttc ggagtaatgc aggttatgcg acaaataact
     6721 cggcatatag gtcagcaaca tgtagtaagt cacgttggtg gcaattacca gaccaataca
     6781 tgtcaacagg ctgcgccagt atttagtggc aatctcttta aacgagactt tcgggccatc
     6841 ctgcaaacct tcgcggtcgc cctgttccag tttatcaaca tgctgctgga acgccggagt
     6901 ttcttccagc gcatggcgca ggtaaagccc gataatccct aacggcagag caataaagaa
     6961 cggaatacgc cagccccagt cgaggaagtt cgcttcgccg acaatggtcg aaattaacac
     7021 caccacgccc gcacccagca caaacccggc aatagaaccg aagtccagcc agctgcccat
     7081 aaagccacgt ttacggtcag gggagtattc cgcaacaaat atcgacgccc cggtatattc
     7141 accgccgacc gagaaacctt gtgccatctt acagatcaac agcagaatcg gtgcccaaat
     7201 accaatcgtg tcgtaggacg gtattaagcc aatacagaac gtactgatcg acataatcac
     7261 aatagtgata gcgaggatct tctggcgacc atatttatcg cccaacatac caaagaagag
     7321 tccgccaagc ggtcgaatca gaaagggaac ggagaaagtg gcaagtgcag caaccatctg
     7381 cacgctgggg tcagcccccg ggaaaaaaac tttacctaat gcgtaagcaa caaaaccata
     7441 aacaccaaaa tcaaaccatt ccattgcatt acccagtgat gctgcggtaa tggctttacg
     7501 cagtttaccg tcatcaataa tggtgacatc acgaagggta atcggtttta cttttttcct
     7561 tttcagcata gctttcctcg cagagttggc ccatttccgc aaacgccaga aaaatcagcg
     7621 cgacggccgc aaaaggggag cgggaattgc aggatgcaat ctctgtaaca agcgtagcgg
     7681 gtttgcttac accctccggt taaggaatgt acaatcaaac cagttgacgc ctgaattggg
     7741 cagttaatga cctttttacc ctaccagcgt ttaaatttgt gatcaacttc acacatggat
     7801 ttcgtttagg actcattgat gtaactgatt atttacacca aatttacaga gaagatttcc
     7861 tcgcgcattg cccccgccag acgctgcgct gaacagagtc gtggcatccc cagcgcgaca
     7921 gtttgccagc gttgggtcac tgaccagcaa acgggttgtt tatctgccgt ggtccacttt
     7981 cccagccaac ttaaaaaatc agcatgatcc ccgcccgtcg gaacggtcga agaggcaaac
     8041 cactgatggt aatagtgacg cgtggtcggg gccgcattga tactctctgc cagatgttgt
     8101 gtcgctttca cgcgcaaatc acccagcaac aacgccaccg cttgcgggct tcccagacga
     8161 caggcatcgc caaatgcccc ccaacgcaac tcttcgagaa cgataaaaca gcgcccaggc
     8221 aacgaccagg cgttatacgc cccagcacgc cagcgggtaa aaatttgctc gctctgttca
     8281 cccgcctgca ccagtaggcc tcgttgcgtt aacgcctttt ccttccagcg acaacgggct
     8341 gaaaaccact catacaaggc ggcaatttgc tgggggagct ccgggaaagc agagtgctgg
     8401 cggtgatcca ttttttgcag cagcgccaga tgctgcgcga tctgcgtcag tgccagatga
     8461 ccgggatcca gcatcgacgc cagtttttcc accaatgtca gacgcagact aagcggcgca
     8521 tttaacatcc cgccgttcgt ccatgtctga agctgcacgc gcgcaagaag ttcctgcatc
     8581 agtcgttcac gaaactgctg ctgatgggct tgtggaaggc gggcatcatc gccctgcgcc
     8641 agatccacta aaaagcgggg ataaaccgac tccagcacgc gaccggggcc gtccagtaac
     8701 gtcttggtca atatcgttct gccgtgaaaa gtgtttgaat atcatcgcgt aacagctgag
     8761 cgtcggtgta aatccagccg tgagtcatca cagtctgctg caattgctgt tgcatcagcc
     8821 tgaccaccga ttcattttgt tgacgcagag ccagactttc gcgtaaacgc gtctgtaatt
     8881 ccgtcaaaca tgaagcgaac tcagcgaaaa aagtattcat gcctgccgta acagattcat
     8941 cgacctgctc tgccagaact ttagccattt gttggcaata aagatcgact tctgcgctta
     9001 atgctcgttg caacacactg taatcaaccg tttctgtcgg ggatttctca tttccccgtc
     9061 cccagtcagg ctgattcaac cagcgcgaaa aagtctcacg cacaacgcct aatcgcgtgc
     9121 tctgctcgtc cgttgcttcc tggcgcgaaa tgacttcact gaacagctgg cgagtgttga
     9181 agtggggaac tacgccgtga aaaacaggaa aatgaaaccc aggacgaaac cctgactcgc
     9241 tcaattccat tttgacttgt tgctcaatgg ggcgaataac atcggttaac acccggcaaa
     9301 gggtggattc aagctcggca aaacgcagcg taaagtcgcg actgatggtg ttctgcgccg
     9361 tctgtaacag tgtctcacag cgggtacgaa tctcgcttaa cggctccgaa tcatcctgaa
     9421 acaaggcggc taactgcacc ttcagcgcat cttgctgttg acgcagaaag tggttggcgg
     9481 aggtcagggc cagctcgatt tcatgtttaa tctcgccgct cacctgcgcc tgattgagtt
     9541 gcaatagctg caaactttct tcgatctggt ggatattttg ccgcaattgt tcacaagcga
     9601 cgtttaaccc gtgcgcacga aaatccaggt attcccgcgc ctgctgcgcg taattcaaca
     9661 gtttatgcgc ggcagatcgc agagcataca acgaggcgtt agcgtaagcg gcatgaagca
     9721 acatctgaat tggctgggcg aacagcgaat cttcccacaa ctgatcggca gcatgacgaa
     9781 tatgttcgag gtccgccaga tcggcatgac gccagcgcct gccgagcgcg gcatgggcaa
     9841 aatcttccac ccagcgttgt tgctctggcg gtggtaactt accgttgttg gctaactcat
     9901 ggcgcgcccg atttgccagg tagccccaca tcgacgacac cggaaatatc tgctgtggcg
     9961 taatacagcc tttcatcagc gtcccggaaa tcagtgcccg cacctggtcg gcgtcgtcac
    10021 tgttacgatc ctgttgatcg aacttattga ccagcacata cagcggcacc gattgcccca
    10081 ccgccaaaat cgcctcacgg acctcttcat cggagatcga tttcagttgc gtataatcca
    10141 gcaccgccag taccgccgag gcgcgtgcca gctgctggtt aagcattttt tgcagatgcg
    10201 gttgcccggc ttcatttggc ccgggggtat ccagtaacgt caattgaccg ggataacttt
    10261 ccagccccgc cagatggaca aactccactt caatcacggg aatatgctca atggcggcgt
    10321 aagcagaaaa aggaaaatcg acgtccagcg ccttcgccag tcgcaccaaa tcattcaaac
    10381 ttttcagaca atgaaaaata ggctgggcac ccagataata tttttcgaaa gcgacgccat
    10441 tttcgatccg ctgcataagc gcacgcatat ctttatctat ttccagcaca tcggtcagat
    10501 gcttaatatc gcaatcacgc aggcgctgtt gtaatttttg aattaaacaa tcgattggcg
    10561 cgacatgtga aaaatgcagt accggttcct tttggccggg cgtatggcga ataagcgtcg
    10621 gcagcgcagt cattgggcga ttgcgattag gcagtacctc cgtaccaaca atggcattaa
    10681 tggtggttga tttccctgct ttcatggtac cgacaatcgc cagcaccatt tccagtcggg
    10741 aaattttacg caactcatta ttcagcatcg cgtgacgctc ggcgatatta ggctgactcc
    10801 agggtaaagc cagttgtggc gcatcgtctc cgggtacaga gagaggcatt ttttccagta
    10861 actgcaactg ttggcgagaa agctgtaaca gccgttcagc ctcctgactt aactcataca
    10921 gggtctgtgt gtacatagaa aattcttcct taaagcaaat tttattactt ttattt
//
LOCUS       DAZKIP010000136        10853 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZKIP010000136 DAZKIP010000000
VERSION     DAZKIP010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.137"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..192
                     /locus_tag="ACHOUK_003951"
     CDS             <1..192
                     /locus_tag="ACHOUK_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFP9899300.1"
                     /translation="DRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRYIPQA
                     QDGEDFRNHLQYKLTLPTH"
     gene            403..726
                     /gene="ybjQ"
                     /locus_tag="ACHOUK_003952"
     CDS             403..726
                     /gene="ybjQ"
                     /locus_tag="ACHOUK_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFP9899301.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            723..1553
                     /gene="amiD"
                     /locus_tag="ACHOUK_003953"
     CDS             723..1553
                     /gene="amiD"
                     /locus_tag="ACHOUK_003953"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFP9899302.1"
                     /translation="MRRVFWLIAVALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(1550..2563)
                     /gene="ybjS"
                     /locus_tag="ACHOUK_003954"
     CDS             complement(1550..2563)
                     /gene="ybjS"
                     /locus_tag="ACHOUK_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFP9899303.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(2662..4092)
                     /gene="ybjT"
                     /locus_tag="ACHOUK_003955"
     CDS             complement(2662..4092)
                     /gene="ybjT"
                     /locus_tag="ACHOUK_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFP9899304.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNFRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRRTLKEEEKLVNSSDWGYDTQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKRIARLAEQSTD"
     gene            complement(4103..5104)
                     /gene="ltaE"
                     /locus_tag="ACHOUK_003956"
     CDS             complement(4103..5104)
                     /gene="ltaE"
                     /locus_tag="ACHOUK_003956"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFP9899305.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKDAWEFTRERN
                     LALHVDGARIFNAVVAYGSELKELTQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMTGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(5141..6859)
                     /gene="poxB"
                     /locus_tag="ACHOUK_003957"
     CDS             complement(5141..6859)
                     /gene="poxB"
                     /locus_tag="ACHOUK_003957"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFP9899306.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEID
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(6992..7960)
                     /gene="hcr"
                     /locus_tag="ACHOUK_003958"
     CDS             complement(6992..7960)
                     /gene="hcr"
                     /locus_tag="ACHOUK_003958"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFP9899307.1"
                     /translation="MTMPTNQCPWRMQVHHIKQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLTRVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAQEFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(7972..9624)
                     /gene="hcp"
                     /locus_tag="ACHOUK_003959"
                     /gene_synonym="priS"
     CDS             complement(7972..9624)
                     /gene="hcp"
                     /locus_tag="ACHOUK_003959"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFP9899308.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(9768..10667)
                     /gene="lysO"
                     /locus_tag="ACHOUK_003960"
     CDS             complement(9768..10667)
                     /gene="lysO"
                     /locus_tag="ACHOUK_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFP9899309.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         2611 a         2881 c         2899 g         2462 t
ORIGIN      
        1 gatcgggcgt ttatatacga atttgcaaga atgatcttcg cttatattaa tgacaaaagc
       61 attgagatct tattactttc ttgttttgcg gcggcggacc aaaaagcaat ccagcggtat
      121 atacctcagg cgcaagatgg ggaagacttt aggaatcatt tacaatataa gttgactctt
      181 cccactcatt aatgttgccg gcacaacata atagggcagg taagcggtgc tccataatga
      241 aaccaatatc cggttacgaa acagataatt gtacaggctt atgtagctgt ttttgacaca
      301 atgagatgat ttcctaaact gatcaaaacc ttcttcccaa catggcatat cagtccgata
      361 atgctttaga gatcgaaggc ttaacaaaca aggagatcga tcatgcaatt ttccacaact
      421 ccaactctgg aaggccagac catcgttgaa tattgcggtg tggtgactgg cgaagcgatt
      481 ttaggcgcga atattttccg tgatttcttt gctggtattc gcgatatcgt tggcggacgc
      541 tccggtgcct acgaaaaaga gctacgtaaa gcgcgggaga tcgcctttga ggagcttggc
      601 tcccaggcgc gggcgctggg ggccgatgcc gtagtcggta ttgatatcga ctacgaaacg
      661 gtcgggcaaa acggcagtat gctgatggtt agcgtcagcg gtacggcggt gaaaacgcgt
      721 cgatgagaag agtcttctgg ctgatcgctg tcgctctgtt actggcaggg tgcgcaggcg
      781 aaaaaggcat tgtcgagaaa gagggatatc agcttgatac ccgacgccag gcgcaggcgg
      841 cgtatccgcg cattaaagtg ctggtgatcc actacaccgc agatgatttt gatagctcgc
      901 tggcgacact gaccgataag caggtcagct cgcattatct ggtccctgcg gtaccaccgc
      961 gatacaacgg taaaccgcgc atctggcaat tggtgccgga acaagaactg gcctggcatg
     1021 cggggattag cgcctggcgc ggggcaacgc gccttaacga cacctctatt ggcattgagc
     1081 tggaaaaccg tggctggcaa aaatcggcgg gggtgaaata ttttgccccg tttgaaccgg
     1141 cacagattca ggcgcttatt ccgctggcga aagatattat tgcccgttat cacatcaagc
     1201 cggaaaacgt agtggcacat gcggatatcg ccccgcagcg caaagacgat ccggggccat
     1261 tatttccctg gcagcaactg gcgcagcagg ggattggtgc ctggccggat gcgcagcggg
     1321 tgaactttta ccttgccggg cgcgcgccgc acacaccggt agataccgcg tcattgctgg
     1381 agcttttggc gcgctacggt tatgacgtta aacctgatat gacaccgcgc gagcagcggc
     1441 gcgtgattat ggcgttccag atgcatttcc gcccgacgtt atataacggc gaagcggatg
     1501 cagaaactca ggcgattgcc gaagcgttgc tggagaaata cgggcaggat tagcgcggca
     1561 gttttccgtg gtcgcgcagc caggcggcag ttttctcgat accttcatcc agggtgatga
     1621 ccggctgata acctaactct tcctgtgccc gcgtaatatc cagcgtaaag tcaaaattaa
     1681 gcttcgagac gccgtagtgg gtcagcggcg gctcttttgc tgacttgcgg cctaaacgct
     1741 ccatgctgcg ggcgatcata tccagcatcg ggtaagggac ggaacgaata cgacagtcaa
     1801 tattcaactc gtcgatcagc ttctgcacga tgctgcgcag tgtgcgatgc tcgccgttgg
     1861 tgatgttgta cacacgcccg gaaggtagct tatcgcaggc ttcctggctt gccagccaca
     1921 tggcgtgcac ggcattttca tagtaggtca tatccaccag cgcactgccg ccatgcggta
     1981 acagaatgct gccgtagtgg tgcatcatat gcgcaagacg ggggataaag actttatcgt
     2041 gcggtccgaa cagactttgt gggcgcagaa tagtaaagcg cgtttgtgga ttcgcctgcg
     2101 aaagcatatt gatcacttct tcgctggccg ctttgctgcg ggcaaactcg ttggcgaagc
     2161 ggtgagggcg aaaatcttct ttaatatcgc gatggtggtg ataatcgaag tacagggagg
     2221 gagaagagat atgaataaag ttacgtacac cccaggcgac agcccattca cctaggcggc
     2281 gagtggcgcg aacgttagcc agatcgaaag cctgttgtgt cccccaggga gaggtaaagc
     2341 tggagcagtg ccacagcgta tcaatgcccg cgagcatcac tttagcttgt gatgagacca
     2401 gctcggtcag atccgccgga acaaactctg cgcccatttt ttccagcaat ttacccattg
     2461 cctcgttgcg accggtcgct cgcacgctga tgcctttctg gcataaaaac tctaccgcgt
     2521 ttcgacctaa gccgctggtg gcgccggtaa ccagtacctt catatcaatc cactgttgtt
     2581 gagaaaatga cgtgcgcatt cttccgtgat ttcccccttg atgcaatggg aaacatgaaa
     2641 gaataacgca ggttttgtcg attaatctgt gctttgttct gccagtctgg cgattcgttt
     2701 tgccattccg cgaaaaataa acagatgcgc ggggatcatc aataaccagt aaaacagccc
     2761 cggcataccg tgcggatgcc agaaagcgcg gacatcgata gtacgatagt cgcctttatc
     2821 ttccagggta aaacacagtc gtcccagccc cggtgctttc atgccaaata acaacgtaag
     2881 ttgtttttcc ggttcaacga caatcacttt ccagctatcc accgcatcgc cagtttgcaa
     2941 atattcgcgc tccggacggc ctttcgctaa tttatgaccg atcgcacggt ccatcaacgc
     3001 ccgtgtctgc cacaaaatat tgccaaagaa ataacgctct ttaccgccga tttggttcac
     3061 cacctgccat aaagccgcaa ggctggcgga cgttttaacg gtaaatcccg cctgtttggc
     3121 aaaataacca tactctggtc gccagcgggc aaaggcctga gtgtcgtatc cccagtcgct
     3181 ggagttgacc agcttttctt cttctttcag ggtgcgacgt accgcgtcat cgaaagcgat
     3241 cagccgttgt gggatgagcg cacgtagcgc ggtgtcatcc gccagcagat cgtgtttcag
     3301 cccctgaatc aacgccctgg cggtggtggg cggtacggaa gtaatcacat tgagaaacca
     3361 caccgaaatc cagcgggtgg ggagggggat ggggatcaac cagcggcgct taccgctcac
     3421 cgccataaaa tgttcaaact gttgctgata actgagcacc tctggtccgg cggcttcgaa
     3481 gatgcggtgt tcgttggctg gatggtctaa cagcgccacc agatagtgca gcaagttttc
     3541 cagcgcgatg ggcgtggtgc gtgaacgtac ccagcgtggc ggcgttaaca ctggcaggtt
     3601 gtagaccata tcgcgcatga cttcgaacgc tgctgaacct gcaccaacga ttattccggc
     3661 acgcaattcg gtcactggta cattcgcttc acgaagaatg tccgccgtag cctgacgcgc
     3721 gcgcagatgg tccgactgct catgtggcgg ggcctgcaac gaactgagaa agattaattg
     3781 cttaactggt acttcacgta gcgcatcgcg gaagttgaga gccacctggc gctcctgagc
     3841 gataaaatcg ccgccttcgc ccatgctatg caccagaaaa tagaccgtat cgatatcctg
     3901 taacagggcc ggaaggttat ccggccagct gagatcgact ttatggcaac tgacgtttgc
     3961 cagttgcagc tttgcaagcc tgtcgacatg acgtgccgcc gccaggatct gatgcccttg
     4021 ctggctgagt gtgcgcacca gatgctgacc aatgtagcca ctggcaccaa gaactaaaat
     4081 gcgttgcggc acgtctccct ccttaacgcg ccaggaatgc gcgccagtgg gcggcgactt
     4141 ctgccagttg ttcgcgcgag acgtcaagat gggtcaccag gcggacaatc ggcgaggcgt
     4201 taatcagtac gtttctcgct ttcatgtatt cgcctaacgc ggcagcattt tcttcgccga
     4261 cgcgaacaaa cagcatatta gtgtcctgac gcatcacatc cgcgcctgct tcacgcagtt
     4321 gctccgccat ccaggcggcg ttgtcgtgat cttcctgcaa ccgtgcgacg ttatttttca
     4381 gcgcatacat cccggcggca gccagaatgc cggactggcg catcccgcca cctgtcattt
     4441 tccgccagcg aatggcacgt ttaatgtaat cacgattacc gacgagtaat gaaccgactg
     4501 gcgtcccaag acctttcgac aggcaaatgg tgaacgaatc acaatattgc gtgagctctt
     4561 tcagttcgct gccgtaagcc accacggcat taaagatgcg cgcaccgtca acatgcagcg
     4621 ccagattgcg ctcgcgggta aattcccatg catctttaag gtattcacgc ggcaacactt
     4681 tgccgttgtg ggtgttttcc agactgagta atttggtgcg ggcgaaatgg atatcgtcgg
     4741 gtttgatttt catcgccact ttatccagcg gtagcgtgcc gtcggcagcc gcgtcgatgg
     4801 gttgcggttg aatactgccc agcaccgccg cgccaccggc ttcaaacaga tagttatgcg
     4861 cggcctgacc gacaatatac tcttcgccgc gttcgcagtg actgagcaga gcgaccaggt
     4921 tggcctgagt gccggtaggc agaaaaatgg cggcttcttt accggaaagc tctgctgcat
     4981 agtcctgcag agcattaacg gtagggtcgt ctccgtaaac gtcgtcccca accggggcgg
     5041 ccatcatcgc ttcgagcatg gcgcggctcg gtcgggttac ggtatcactg cgtaaatcaa
     5101 tcatggcatg tccttattat gacgggaaat gccacccttt ttaccttagc cagttcgttt
     5161 tcgccagttc gatcacttca tcaccgcgtc cgctgatgat tgcgcgcagc atatacaggc
     5221 tgaaaccttt ggcctgttcg agtttgatct gcggtggaat ggctaactct tctttggcga
     5281 ccaccacatc caccaatacc ggaccgtcga tggagaaggc gcgttgcagg gcttcatcga
     5341 tttcagaggc tttttctaca cgaatacccg taattccgca cgcttcggca atgcgggcaa
     5401 agttggtgtc gtgcagctca gtaccgtctg tcaggtagcc tccggctttc atctccatcg
     5461 ccacaaagcc cagcacgctg ttattaaaga cgataatttt caccggcagt ttcatctgca
     5521 ctaccgagag gaaatcgccc atcaacatgc taaaaccgcc atcgccgcac atggcgacca
     5581 cctgacgttc cggctcggtt gcctgcgcgc ccagcgcctg cggcatggcg ttagccatcg
     5641 aaccgtggtt aaacgaacct aacaggcgac gcttgccgtt cattttcaga taacgcgccg
     5701 cccacaccgt tggcgtgccg acgtcacagg taaagatggc gtcatcggcg gcaaaatgac
     5761 taatttgctg cgccagatat tgcgggtgaa tggctttctc gctcggttta gctaaatcgt
     5821 ccagcccttt gcgggcatcg cggtaatctt ccagcgcttt atccagaaac ttgcgatcgg
     5881 ctttttcttc caccaatggc agcaatgcac gcagggtcga cttgatatcg ccgaccagcg
     5941 ccatatccac cttgctgtgt gcgccgatgc tggctgggtt gatatcaatc tgaataattt
     6001 tggcatcggt cgggtagaac gcacggtagg ggaactgagt accgagtagc actaacgtgt
     6061 cggcgttcat catggtatgg aaacctgacg agaatccgat taacccggtc attccaacat
     6121 catacggatt atcgtattcg acatgctctt taccgcgtag ggcatgaaca ataggtgctt
     6181 taattttccc ggcaaactca actaactctt tatgcgcccc cgcgcagccg ctgccacaca
     6241 tcagagcgat attgctggaa taacgcagca gttgcgccag tttgcgtaac tcttcttctt
     6301 ccggcgtcac gactggctgt ggcgcatgat accagtgggt agttgcccct tctggcgcag
     6361 gttttaacgc cacgtcgccc ggtaacacga caacggaaac gccacggtta agcaccgctt
     6421 tgcgcatggc aatcgccagc acttgtggga tctgctccgg gctggaaacc agctcgcaat
     6481 agtgactaca ttcgcggaat agctcttgtg ggtgggtttc ctggaaatag ccgctgccaa
     6541 tttcgctgga aggaatatga gcggcaatcg ccagtaccgg aacgtgattg cggtggcaat
     6601 cgaacaggcc gttgattaag tgcaggttgc cggggccgca cgatccagca cagaccgcca
     6661 gttctcctga aagttgtgct tcagcgccag cggcaaacgc cgccacttct tcatggcggg
     6721 tggacatcca ctcgatggtg cccatgcgat taagactgtc gctaagaccg ttcagagagt
     6781 cgcccgtgac tccccagatg cgtttcacgc ctgccgattc gagtgttttg gcgatataag
     6841 ctgcaaccgt ttgtttcatg gttctccatc tcctgaatgt gataacggta acaagtttag
     6901 ttcatctgac ggagggggaa gggatgggag agaaaggagg cactaacggt taaatagccc
     6961 gatgaaagga atatcatcgg gcataaggcg attatgcgag aaccaaatcc ccctgcggat
     7021 ggcaggagca ggccagtacg taaccttcag cgatttcggc gtcggtcagc gtcattgtac
     7081 tgctcaccgt atattcaccg gaaaccactt tcgtcttaca gcagccgcaa acacccgcgc
     7141 ggcaggcggc gacaaccgga acgttattgc tttccagcgc ctccagcagc gtggtgccaa
     7201 caggagcgta aaattcttgc gccggttgca gtttggtgaa tttcagaccg ctggtcgccg
     7261 cttccgctac tggggtgaag aatttctctt taaagaaacg cgtcacgccg agtgctttca
     7321 cttcctgctc tacccaatcc atatacggag ccgggccgca ggtcatcacg gtacgtgaag
     7381 ctaagtcagg aacgcgagtc aggagttcgc gagtgagacg accagcgata aagccttcgg
     7441 taacgttatt ttccgccacc agcgttaccg gatagttacg ccactcatcg gcgaaaataa
     7501 catcctgcgg cgtacgcacg ttgtagatca cccgcacatc ggcctgtgga cggttcttcg
     7561 ccagccagcg acgcatcgac ataatcggcg tgacgccgca gcctgcagcc agcaacagga
     7621 atttatcttc tgctttatcg tcgcaggtaa attcccccat cgcgtctgaa agccagagat
     7681 aatcaccgcg ttttacatcg cgggtcagcc actgggagcc gacaccgtca tcaatccgcc
     7741 gcacggtcag ggtgatatat tcactcacgc ctggcgtgga ggaaatggtg taagcacgca
     7801 gcgtttccgc tgagttacgc acgctgacca gtgcatattg cccggcgcgg tatgggtagt
     7861 aatcgtggca aatcagggaa atcgtccaca catccggcgt ttcttgctta atgtgatgaa
     7921 cctgcatccg ccacgggcat tgattcgttg gcatcgtcat cgacaaactc cttacgcgct
     7981 caacagttgc ttcatgtctt cttcaacagt ggtgatagaa cgcaggccga atttctcgtt
     8041 cagcaccgcc agcagatctg gtgtcaggaa accaggtgca gtcgggccgg tgacgatatt
     8101 tttcacgccc agagaaagca gcgtcagcag aatgacaatc gctttctgtt caaaccagga
     8161 gagcaccagc gacagcggca gatcgttgac accgcagccc agtttttctg ccagcgtaac
     8221 cgccagaata attgctgagt aagcatcgtt acactgaccg gcatccacca gacgaggcag
     8281 accttcgata tcgccaaact caagtttgtt aaagcgatat ttaccacagg cgagagtcag
     8341 gatcaggcag tcatctggca cgctggtggc gaaatcggtg aagtagtgac gttcgccgcg
     8401 tgcgccgtca cagccaccaa gcaggaagat atgacgcagt ttttcacggc tcaccagatc
     8461 aattagcgta tccgctgcgc caagcagcgt ctggcggcca aagcccacgg tgatcaggtg
     8521 cggaatttcg ctgtatggga agcctgccat ttgttgcgcc tgggcaatga ccgcagagaa
     8581 atcttcgcca tccagatgac gcacgccagg ccagccaacg atgctgcggg tccagatacg
     8641 atcgtcataa gcgcctacgg ttgggtcgat gatgcagttc gaggtcatca cgatggggcc
     8701 agggaaacga gcgaactcca cttgctgatt ctgccagccg ctgccgtagt taccgaccag
     8761 atgcttgaat ttacgcagct ccgggtagcc atgcgcaggc agcatttcgc cgtgggtgta
     8821 gacattaacg cccgtgcctt cggtctgttc cagcaggttg tagagatctt tgagatcgtg
     8881 accggaaatc aggatgcatt tacccgccgt cgctttgacg ttgacctggg ttggcgtcgg
     8941 gtgaccgtat ttaccggttt cgcctgcatc cagaatgctc atcactttga agttcatctg
     9001 gccgatttcc attgaacact caagaagcgc gttcatatcg gcaggccagg tccccagcca
     9061 cgccatgatt ttatgatact gggcataaat atcgttgtcg tattgaccga gaacgtgtgc
     9121 gtgttccata taggccgccg cacctttcag gccatacagg cacagcagac gcaggccgag
     9181 aatgttttcg ccaatcgccg ctttatcttt gttaggggta aattctgctg cctgacgttg
     9241 cagttcgccg agatcgtcgc tcaccagttg caggtcagcc atcgggttat cgacgcgggc
     9301 gttggcatct acagccaggc attgtgcttt cagcgcctcg cgcaaggcaa tcgcttcacg
     9361 agcgtagccg acaatacgcg gagaatcgaa gttaacgttg gtcagggttg agaaaaaggc
     9421 acgtggcgcg aagctgtcga catcgtggtt gatgatgccg tattcacgcg cttttaccgc
     9481 ccaggcagaa agcccttgca gcgccgcgat gagtaaatcc tgaaggtcag aagtttccgc
     9541 cgttttacca cacatcccct gcgcgtatga gcagccgttt cctgccggag tacggatagt
     9601 ttgttcacat tgcacacaaa acatgatcac accttttaaa gttatattta atatacatgt
     9661 ttaaggttaa gacgcttaac gcggggataa aagggatttt tcatgcaact ttaagggaga
     9721 ttgatttagc gcaattttgg cggcagggat ctaccgccag agaggtatta cgcggagaaa
     9781 aaggcgatga ggatcggcac taacaggcta agaataaaac cgtgaacaat tgccgccggg
     9841 accatatcca gcccgccagt acgttgaaga acgggcaggg tgaaatccat cgacgtcgca
     9901 ccgcataagc ccagtgcggt agagcggctg cggcgaatca gcccagggat caacataatt
     9961 gcaatcagct cacgggccag atcattaaaa aacgccgcgc tcccgattac cggaccaaaa
    10021 gattcggtca ataaaatacc ggaaagagaa taccagccga aaccagaggc cattgccagc
    10081 gcggtattga tgggtagatc aagaataaag gcgttaatta aaccaccaat taatgaactg
    10141 gcaaccacca ccacggcgac aatcattccc cggcgattaa ggacaatctg ctttaaggtc
    10201 atgccattat tgcgcaactg aataccgacg aggaaaagta gcagaattaa cgtgtattca
    10261 ctggcttcgg tcgcgtgttg taagaaagcc agtccactca ggccaatggc aaaaccaatc
    10321 actactacgc cgcacagttt tagcgactca agcgccatcg caatacgcga cgggagtttt
    10381 tcttgctgat ggtggttgcg ccacggcaga ccgcgctcca gccacataag ggcggcaata
    10441 ttacacagta aaataacggt aatactgacg gcagaataat gcagaatcgc caacaggtta
    10501 ctggcgaggt tatcgagaaa cgccagactg atacccataa aaaagagaat aaggtaaacc
    10561 atccagctta atagctgatt aataactttt aacgcagctt gttggcgaag cggaatgagg
    10621 taacccacaa tcaggggaac cagaatgatt aacaacccag aaaacatgaa aaaccagccc
    10681 ttgcaaagaa agaagtcgaa atgcgcgatg acacactact gaaagcggaa ggacgagtaa
    10741 agttgcaatt aaaaggaaat gttatgcatc agagggggat ttttaattct atatgagtgt
    10801 ttagcattgt tgcgctcctt tcatgtgcaa tgccatataa agccactggc gca
//
LOCUS       DAZKIP010000137        10298 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZKIP010000137 DAZKIP010000000
VERSION     DAZKIP010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.138"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            143..718
                     /gene="ssuE"
                     /locus_tag="ACHOUK_003961"
     CDS             143..718
                     /gene="ssuE"
                     /locus_tag="ACHOUK_003961"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFP9899310.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAA
                     EDLLYARFDSPALHTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLHSLRGNAHA"
     gene            711..1670
                     /gene="ssuA"
                     /locus_tag="ACHOUK_003962"
     CDS             711..1670
                     /gene="ssuA"
                     /locus_tag="ACHOUK_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFP9899311.1"
                     /translation="MRNIIKLALVGLLSVSTIVVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLLYVG
                     VEPPKPKAEVILVAENSPIKTAADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1667..2812
                     /gene="ssuD"
                     /locus_tag="ACHOUK_003963"
     CDS             1667..2812
                     /gene="ssuD"
                     /locus_tag="ACHOUK_003963"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFP9899312.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVDFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVENDFIPRRVAQS"
     gene            2824..3615
                     /gene="ssuC"
                     /locus_tag="ACHOUK_003964"
     CDS             2824..3615
                     /gene="ssuC"
                     /locus_tag="ACHOUK_003964"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFP9899313.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVTVWQLASSVGWLSTRILPSPEG
                     VVMAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3612..4379
                     /gene="ssuB"
                     /locus_tag="ACHOUK_003965"
     CDS             3612..4379
                     /gene="ssuB"
                     /locus_tag="ACHOUK_003965"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFP9899314.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGESETRLRKQG"
     gene            complement(4422..7034)
                     /gene="pepN"
                     /locus_tag="ACHOUK_003966"
     CDS             complement(4422..7034)
                     /gene="pepN"
                     /locus_tag="ACHOUK_003966"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFP9899315.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLCLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7300..8502
                     /gene="pncB"
                     /locus_tag="ACHOUK_003967"
     CDS             7300..8502
                     /gene="pncB"
                     /locus_tag="ACHOUK_003967"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFP9899316.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLEIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8671..10071
                     /gene="asnS"
                     /locus_tag="ACHOUK_003968"
     CDS             8671..10071
                     /gene="asnS"
                     /locus_tag="ACHOUK_003968"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFP9899317.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQTSKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTEAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2310 a         2637 c         2782 g         2569 t
ORIGIN      
        1 gatatataaa accctttctt tagtttattt tcagaaaaag atacacaaaa tttatatttg
       61 gcattttctt gtctctccga cagactgagc gaattgttat caatgttaac aaaacaagaa
      121 caattggtta taaggagaga gtatgcgtgt catcaccctg gcgggtagtc ctcgctttcc
      181 ttctcgctcc agctccttgc tggaatatgc gcgggaaaaa ctaaatggcc tggatgttga
      241 ggtttatcac tggaatctgc aaaacttcgc cgcggaagat ctgctttatg ctcgtttcga
      301 tagtccggca ctccatacct tcaccgaaca gttgcaacag gctgacgggc tgattgtcgc
      361 cacgcctgtg tataaagccg cttattccgg tgcgctgaaa accctgctcg acctgctgcc
      421 agaacgtgcg ttgcaaggca aagtggtgct accgctggcg acgggcggta cggtggccca
      481 tctgctggcg gtcgattatg cccttaagcc agttttaagt gcgctgaaag cccaggagat
      541 cctgcacggc gtgtttgccg atgactcaca agtaattgat taccatcaca aaccccagtt
      601 cacgccgaat ttacaaaccc gtcttgatac cgcgctagaa actttctggc aggcattgca
      661 ccgccgcgat gttcaggttc ctgatcttca ttctctgcga ggtaatgccc atgcgtaaca
      721 tcattaaact ggcgctggtg ggattgctta gcgtctctac gattgtggtt gctgcagaat
      781 cctcgcctga agcgttacgt ataggctatc agaaaggcag tattggtatg gtattggcaa
      841 aaagccacca gttactggaa aaacgctatc cgcaatcaaa aatctcgtgg gtggagttcc
      901 ccgctggccc gcaaatgctt gaggcgttaa acgttggcag tattgatctc ggcagtaccg
      961 gggatattcc gccaatcttc gcccaggctg ccggggctga tttgctgtac gtgggcgtcg
     1021 agccgccgaa gccaaaagcc gaggtgattc tggtggcaga aaacagcccg atcaaaaccg
     1081 cagccgatct taaaggccac aaagttgcgt tccagaaagg ttccagttca cacaatcttt
     1141 tactgcgcgc actacgccag gccggactta agtttactga catccagccc acttatctga
     1201 cgccagctga tgcccgcgcc gcgttccagc aaggtaacgt tgacgcctgg gctatctggg
     1261 atccctacta ctccgctgca ttattacagg gcggcgtgcg ggtgttgaaa gacggcaccg
     1321 atctcaatca aaccggatcg ttttatctgg cagctcgccc ctatgcagaa aaaaacggcg
     1381 cttttattca gggtgtactg gcaaccttta gtgaggccga tgcgttaacc cgcagccagc
     1441 gcgagcaaag tatcgcttta ctggcaaaaa cgatgggctt accggcaccg gtgattgcct
     1501 cttacttaga tcatcgccct cctaccacca tcaaaccggt taacgccgag gttgccgcct
     1561 tacagcagca aacggcagat ctgttttatg aaaatcgtct ggtgccgaaa aaagtcgata
     1621 ttcgccaacg catctggcag cccactcaac tggaaggaaa acaattatga gtctgaatat
     1681 gttctggttt ttaccgaccc atggtgacgg gcattatctg ggaacggaag aaggttcacg
     1741 cccggttgat cacggttatc tgcaacaaat tgcgcaagcg gcggatcgtc ttggctatac
     1801 cggtgtgctg atcccaacgg ggcgctcctg cgaagatgcg tggctggttg ccgcatcgat
     1861 gatcccggtg acgcagcggc tgaagtttct tgtcgccctg cgtcccagcg taacctcacc
     1921 taccgttgcc gcccgccagg ccgccacgct tgaccgtctc tcaaatggac gtgcgttgtt
     1981 taacctggtc acaggcagcg atccacaaga actggcaggc gacggcgtgt tccttgatca
     2041 tagtgagcgc tacgaagcct cggcggaatt tacccaggtc tggcggcgtt tattgctggg
     2101 ggaaaccgtc gatttcaacg gtaaacatat tcatgtgcgg ggagccaaac tgctcttccc
     2161 gccgattcaa cagccgtatc ctccgcttta ctttggcgga tcgtcagacg tcgcccagga
     2221 gctggcggca gaacaggttg atctctacct cacctggggc gaaccgccgg aactggttaa
     2281 agagaaaatc gaacaagtgc gggcgaaagc tgccgcgcat ggacgcaaaa ttcgtttcgg
     2341 cattcgtctg catgtgattg ttcgtgaaac taacgacgaa gcgtggcagg ccgccgagcg
     2401 gttaatctcg catcttgatg atgagactat cgccaaagca caagccgcat tcgcccgcac
     2461 ggattccgtg gggcaacagc gaatggcagc gctacataac ggcaagcgcg acaatctgga
     2521 gatcagcccc aatttatggg cgggcgttgg cttagtgcgc ggcggtgccg ggacggcgct
     2581 ggtgggcgat ggtcctacgg tcgctgcgcg aatcaacgaa tatgccgcgc ttggcatcga
     2641 cagctttgtg ctttcgggct atccgcatct ggaagaagcg tatcgggtcg gcgagttgct
     2701 gttcccacat ctggatgtcg ccattccgga aattccccag ccgcagccgc tgaatccgca
     2761 aggcgaagcg gtggagaatg attttatccc tcgcagagtc gcgcaaagct aaggaagaag
     2821 ataatggcaa cgccagtgaa gaagtggtta ttgcgcgttg ccccctggtt tttaccggtg
     2881 ggcatcgtga cggtgtggca actggcctcc tcggttggct ggttgtcgac gcgtattttg
     2941 ccctcaccgg aaggggtggt gatggcgttc tggacgctct cagccagcgg cgaattgtgg
     3001 cagcatctgg cgatcagctc ctggcgggcg ctgattggtt tttcaattgg cggctcgctg
     3061 gggttgattt tgggactgat tagcggactg tcgcgctggg gagaaaggct gctggatacc
     3121 tcaattcaga tgttgcgcaa cgtgccgcat ctggcgctga tcccgctggt gattttgtgg
     3181 tttggcattg atgaatccgc aaaaatcttt ctggtggcgc tcggtacgct attccccatt
     3241 tatatcaaca cctggcatgg catccgtaat atcgatcgcg ggttggtgga gatggcgcgt
     3301 agctatggat tatccggcat accgctgttt atccatgtga tcctgcctgg tgccctgccc
     3361 tcaattatgg tcggcgtgcg ttttgcgttg ggcctgatgt ggctgacgct gattgttgcc
     3421 gaaaccattt ctgccaattc aggcattggt tatctggcga tgaatgcgcg ggagtttttg
     3481 caaacggacg tggtggtagt cgctattatt ctttacgccc tgctcggcaa acttgctgac
     3541 gtcagcgcgc agctgttaga gcgcctctgg ttgcgctgga acccggctta tcatttgaag
     3601 gaggccacgg tatgaatact gctcgtctga accagggcac accattgttg ctcaatgcag
     3661 taagcaaaca ttacgcggaa aatatcgtcc tgaaccaact ggatttacat attccggcag
     3721 gtcagtttgt ggcggtggtg ggccgcagcg gtggtggcaa aagtaccctg ctgcgcctgc
     3781 tggcaggtct ggaaacgcca accgctggcg atgtgttagc gggcaccaca ccgctggctg
     3841 aaattcagga agacacgcga atgatgtttc aggatgcgcg tctgctaccg tggaaatcgg
     3901 tgattgataa cgttgggtta ggccttaaag gccagtggcg cgatgccgcg cgtcgggcgt
     3961 tggctgcggt aggactggag aatcgcgccg gggaatggcc tgccgcactt tctggcgggc
     4021 agaaacagcg agtggcgctg gcaagagcgt tgattcatcg accgggttta ttgttgcttg
     4081 atgaaccgct cggtgcgctg gatgccttaa cgcgactcga gatgcaggat ttgattgtgt
     4141 cactttggca ggagcacggt tttaccgtac tgttggtgac gcatgatgtg agcgaagcgg
     4201 tagcaatggc tgatcgggtg ttgttaattg aagagggaaa aattggtctg gatttgacgg
     4261 tggatattcc ccgtccgcgc cggttagggt cggtgcgatt agcggagctg gaagcggaag
     4321 tgttgcaacg ggtgatgcag cgtggtgaat ctgaaactcg cctgagaaaa caggggtaaa
     4381 atccccgaat ggcggcgcta ttgccgccat tcggggattt attaagccag tgctttggtg
     4441 atcttctcgt acagatcgcc agatagattt tccagccctt tcaactgttc cagcgccgcg
     4501 cgcattttct cctgacgttt ggcatcataa cgtttcaggc gaatcagcgg ttcaatcagg
     4561 cgtgaagcta cctgcgggtt acggctgttc aggtcggtaa gcatttccac caggaactgg
     4621 taaccgctcc catcttcggc atggaacgct gccgggttgc tgcccgcaaa cgcaccaatc
     4681 aacgaacgaa tgcggttcgg gttgctcatg gtaaatgagc gatgctgcaa caggccgcgc
     4741 accgtctcca gcacattcgc cgccgggctg gtggcttgca ggataaacca tttatccatc
     4801 accagaccat cctgatgcca cttgtcgtcg tactcctgca tcagcgcgtc acggcaaggc
     4861 agctgtgcag caaccgccgc agaaagcgct gccagcgcat cggtcatatt gtttgcttca
     4921 tggtattgct tgctcaccag cacgtcagcc agatgcgttt caccaaaagc aaggaagcgc
     4981 aggcaggcat tacgcagagt gcgttttgca atatcttcat gctcaacacg gtactcgctc
     5041 tggtaattcg cgttgtaaat agccagtagc tcatccgcca gttcagtcgc cagagtacga
     5101 gtgagtgctt cgcgtacttc ggcaatagca atcgggtcga tgatatcgaa cagttcagcc
     5161 atttcattga cagaaggcag cgtcaggatt tctgccgcca gcgccggatc aatcttctca
     5221 tcgagcagca ccgcgcggaa agcgtcagcc acatgtaccg gcagagacag cggctgccct
     5281 tgctgatgac gggcgacgtt cagcttgatg taggttgcca gcaaactttg cgccgcatcc
     5341 caacgggaaa aatcattacg cgcatgacgc atcaggaagg tcagttgctg atcgctccac
     5401 ttatattcca gtttcactgg cgcagagaat tcgcacagca gcgcaggcac cggctggaag
     5461 tagacattat caaaaacaaa ggtctgttcc gcctgggtga cgttcagcac ggaattcacc
     5521 ggatgaccgc ctttctgcaa cgggatcact ttgccttcgt tgtcatacaa ttcgatggca
     5581 aacggaatat gcagcggctg tttttctgct tgatccggcg tggctggcgt gcgctggctg
     5641 atggtcaggg tgtactgctc ggtttccgga ttgtagtcgt ctttgacggt cacaaccggc
     5701 gtaccggact ggctgtacca acggcggaaa tgggagagat cgacattcga cgcatcttcc
     5761 atcgcctgaa cgaaatcgtc gcaggtcgct gcactgccat catggcgctc gaaatagagc
     5821 tgcatccctt tctggaagtt ttcttcgcca agcagagtgt ggatcatacg aatcacttcc
     5881 gcgcccttct cgtaaacggt cagggtgtag aaattgttca tctcaatgac catgtccggg
     5941 cggattgggt gcgccatcgg gctggcgtct tccgcaaact gcaatccgcg catggtgcgc
     6001 acattattga tacggtttac cgcgcgggaa ccaaggtcag agctgaattc ctgatcgcgg
     6061 aagacggtta aaccttcttt caggctgagc tggaaccagt cgcggcaggt cactcggtta
     6121 ccggtccagt tatggaaata ttcatggccg ataacgcgtt caatatcgag gtaatctttg
     6181 tcggtggcgg tatcggtgcg ggccagcaca tatttggagt taaagatatt cagaccctta
     6241 ttctccattg cgcccatatt gaagaaatcc accgcgacga tcatatagat gtcgaggtca
     6301 tactccaggc caaagcgttc ttcatcccat ttcatggagt ttttcagcga ggtcatcgcc
     6361 cacggcgcgc gatcaaggtt gccgcgatcg acgtacagct ccagtgctac ttcgcgacca
     6421 gaacgcgtgg taaaggtatc gcgcagtaca tcaaagtcgc ctgccaccag cgcaaacagg
     6481 tagcacggtt tcgggaacgg gtcctgccac tgtacccaat ggcgtccgtt ttccagttcg
     6541 ccttgcgcaa cgcggttacc gttggaaagc aggaagggat atttggtttt atcggcaata
     6601 attttggtgg taaaacgcgc cagcacgtcc gggcggtcga gataatacgt aatatggcgg
     6661 aaaccttcgg cttcacactg ggtgcaaagc gcatcgcctg actgataaag cccttccagc
     6721 gccgtattcg ccgccgggct tatttcatta atgatcttaa gcgtaaaacg ctccggcaaa
     6781 ttactgatga ccagtgcgcc ctcttcttct ttccaggcgg tccacggctc atcattaata
     6841 tgaacagaaa ccagtttgag gtcttcgccg ttgagacaaa gcggagcatc tgatgcacca
     6901 tgacggacag cctggctgac cgcggtaacg accgtctttt gcgcgtcgag gtcaaaggtc
     6961 aagtcaatat cagtaatctg gtaatccggc gcacgataat cgtgacggta tttggcttgt
     7021 ggctgttgag tcataaataa cctttagcat cttttataga gtctggtgtt cagtctattc
     7081 ctgttgcgta aatcgcgcta tgcagaatct tcatcttttc aggtacaaac gcctttattg
     7141 ctacattttt ataacataca ccgcgtaatg ccatcgacca gaaaggtggc atatggtgtg
     7201 atcggggttc aataaattgc gaaacaaggt atactccagc agttcctgaa gatgtttatt
     7261 gtactaaacg ctcctgtacg aggacgctac tgcgcaccta tgacacaatt cgcttctcct
     7321 gttctgcact cgttgctgga tacagatgct tataagttgc atatgcagca agccgtgttt
     7381 catcattatt acgatgtgca tgtcgcggcg gagtttcgtt gccgaggtga cgatctgctg
     7441 ggtatttatg ccgatgctat tcgtgaacag gttcaggcga tgcagcacct gcgcctgcag
     7501 gatgatgaat atcagtggct ttctgccctg cctttcttta aggccgacta tcttaactgg
     7561 ttacgcgagt tccgctttaa cccggaacaa gtcaccgtgt ccaacgataa tggcaagctg
     7621 gatattcgtt taagcggccc gtggcgtgaa gtcatcctct gggaggttcc tttgctggcg
     7681 gttatcagtg aaatggtaca tcgctatcgc tcaccgcagg ccgacgttgc gcaagccctc
     7741 gacacgctgg aaagcaaatt agtcgacttc tcggcgttaa ccgctggtct tgatatgtcg
     7801 cgcttccatc tgatggattt tggcacccgt cgccgttttt ctcgcgaagt acaagaaacc
     7861 atcgttaagc gtctgcaaca ggaatcctgg ttcgtgggca ccagcaacta cgatctggcg
     7921 cgtcggcttt ccctcacgcc gatgggaaca caggcacacg aatggttcca ggcacatcag
     7981 caaatcagcc cggatctagc caacagccag cgagctgcac ttgctgcctg gctggaagag
     8041 tatcccgacc aacttggcat tgcattaacc gactgcatca ctatggatgc tttcctgcgt
     8101 gatttcggtg tcgagttcgc tagtcggtat cagggcctgc gtcatgactc tggcgacccg
     8161 gttgaatggg gtgaaaaagc cattgcacat tatgaaaagc tggaaattga tccacagagt
     8221 aaaacgctgg ttttctctga caatctggat ttacgcaaag cggttgagct ataccgccac
     8281 ttctcttccc gcgtgcaatt aagttttggt attgggactc gcctgacctg cgatatcccc
     8341 caggtaaaac ccctgaatat tgtgattaag ttggtagagt gtaacggtaa accggtggcg
     8401 aaactttctg acagccctgg caaaactatc tgccatgata aagcgtttgt tcgggcgctg
     8461 cgcaaagcgt tcgaccttcc gcatattaaa aaagccagtt aatatcatca gggagctaat
     8521 cggctccctt tttttacctt taattccgaa atctttcgct gcatttgcga attctgcttg
     8581 tctgattgca gatgccaggt aacataggta tccccccatt tacgggtgga caagtgttta
     8641 ttttttccga ctattaacag agagaatatt atgagcgttg tgcctgtagc cgacgtactc
     8701 cagggccgcg tagccgttga cagcgaagtc accgtacgcg gatgggtacg tacccgccga
     8761 gattcaaaag ctggcatctc cttcctcgcc gtttatgacg gttcctgctt tgatcctgta
     8821 caggctgtca tcaataattc tctgcccaat tacaatgaag acgtcctgcg tctgaccacc
     8881 ggctgctcgg tcattgtgac gggtaaagtc gtggcgtcgc cgggccaggg gcaacaattt
     8941 gaaattcaga ccagcaaggt tgaagttgct ggttgggttg aagatccaga cacttacccg
     9001 atggcggcaa aacgccacag cattgagtat ctgcgtgaag tcgctcacct gcgtccgcgc
     9061 acaaacctga ttggtgccgt cgcgcgcgtt cgccatacgc tggcgcaggc gctgcatcgc
     9121 ttctttaacg agcagggatt cttctgggtt tcaacgccac tgattactgc gtctgatacc
     9181 gaaggggctg gcgaaatgtt ccgcgtttct acgctggatc tggaaaatct gccgcgtaac
     9241 gatcagggca aagtggattt cgacaaagac ttctttggta aagagtcttt cctgaccgta
     9301 tctggccagt tgaacggcga aacctacgct tgcgcattat ccaaaattta taccttcggc
     9361 ccgactttcc gtgctgaaaa ctccaacacc agccgtcacc tggcggaatt ctggatgctg
     9421 gagccggaag tggcgtttgc taacctgaac gatatcgcgg gtctggctga agccatgctg
     9481 aaatatgtct tcaaagcggt tctcgaagaa cgcgctgacg acatgaaatt cttcgctgaa
     9541 cgcgtagata aagatgccgt ttcacgtctg gaacgcttta ttgaggccga tttcgcgcag
     9601 gtggattaca ccgaagcagt aaccattctc gaaaactgcg gcaggaagtt tgaaaacccg
     9661 gtttactggg gcgtcgatct ctcttctgag catgagcgtt atctggcgga agaacacttt
     9721 aaagcaccgg tagtggttaa aaactatccg aaagatatta aagcgttcta tatgcgcctc
     9781 aacgaagacg gtaaaaccgt tgcggctatg gatgttctgg ctccgggcat cggtgagatc
     9841 attggtggct cccagcgtga agagcgtctg gacgtgctgg acgagcgtat gctggaaatg
     9901 ggcctgaaca aagaagatta ctggtggtat cgcgatctgc gtcgctacgg tactgttccg
     9961 cattccggtt tcggtcttgg ttttgaacgc ctgattgctt acgtaactgg tgtgcaaaac
    10021 gtgcgtgatg tgattccgtt cccacgaact ccgcgtaacg ccagcttcta atttagcgtc
    10081 tacaagagcc agcacccgct ggctcttttt tatcctttct gtcaagttat ctgtttgtta
    10141 agtcaatcaa tctatttgca accccgccat aacgatcatc ctgttacgga atattacatt
    10201 gcaacattta cgcacaaaaa ataacccgca ttcttattgc ggattagttt tttcttagct
    10261 gatagcacaa ttttcatact atttttttgg cattctgg
//
LOCUS       DAZKIP010000138        10249 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZKIP010000138 DAZKIP010000000
VERSION     DAZKIP010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10249
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.139"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            117..1010
                     /locus_tag="ACHOUK_003969"
     CDS             117..1010
                     /locus_tag="ACHOUK_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899318.1"
                     /translation="MVANINLIKQESYSVVNLEKQLMSNESPKQSLLNDFSHRLKMEG
                     EEQLFHCVNELKYGLSSNNEYDLFKNRETSLWKRKVPESAALAMTGTVYKLFGSRLLN
                     ARNQLDKNLLEMAMQMVYGKDMVTKSGDILIDIQLNKDGSLQSKHYTFDVGEVINSYN
                     IDLFVNASSINQTYLHDKNPGDVISLYGTLDNIPLVVKECTGKNHQINVKAKLPQERS
                     EKVEDMCERMRGGISMFNHTTKTAGNIEHNLQLSFLVDSRPSITNKYSAKDVAPDILV
                     LPVNVYHADFKIKINNAVEKT"
     gene            1017..1931
                     /locus_tag="ACHOUK_003970"
     CDS             1017..1931
                     /locus_tag="ACHOUK_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9899319.1"
                     /translation="MLCAVQKNLHAARKVLSGIIGNVPKNNKPKTIIFDLRFLSAIKQ
                     KVFLGSEQPLFVKHTAMMREACKELPQLVEYVPLACEAHSHRSVALAKTVAAPIMATG
                     VGAALMYATSSGPYYAQSLSGSPDIEMIKKRMAELFNQSDTSVRNKYRPAFDKWNKLV
                     DKLNHERNTVPFACLTTIIATAINETPEGDTNVAVVMGCKSAKDRTISIVLGNSMLQT
                     LFEKRLADDGEIENLFDQQGYFSCDSLTAEEVMMLKDLFDIRVLHLSNKFNVGLQGNI
                     NTDVLQDSFFKNVDFIHESNSYTVGMGV"
     gene            2625..2843
                     /gene="yiiE"
                     /locus_tag="ACHOUK_003971"
     CDS             2625..2843
                     /gene="yiiE"
                     /locus_tag="ACHOUK_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFP9899320.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            3060..3302
                     /locus_tag="ACHOUK_003972"
     CDS             3060..3302
                     /locus_tag="ACHOUK_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFP9899321.1"
                     /translation="MNSLAGIDMGRIILDLSNEVIKQLDDLEVQRNLPRTELLREAVD
                     QYLVNQSQTVRTSAFGIWQGCEEDGVEYQRKLREEW"
     gene            complement(3632..4561)
                     /gene="fdhE"
                     /locus_tag="ACHOUK_003973"
     CDS             complement(3632..4561)
                     /gene="fdhE"
                     /locus_tag="ACHOUK_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFP9899322.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(4558..5193)
                     /gene="fdoI"
                     /locus_tag="ACHOUK_003974"
     CDS             complement(4558..5193)
                     /gene="fdoI"
                     /locus_tag="ACHOUK_003974"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFP9899323.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(5190..6092)
                     /gene="fdoH"
                     /locus_tag="ACHOUK_003975"
     CDS             complement(5190..6092)
                     /gene="fdoH"
                     /locus_tag="ACHOUK_003975"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFP9899324.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNHADEEENNLHEEKDEERK"
     gene            complement(6105..9155)
                     /gene="fdnG"
                     /locus_tag="ACHOUK_003976"
     CDS             complement(6105..9155)
                     /gene="fdnG"
                     /locus_tag="ACHOUK_003976"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(8568..8570),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFP9899325.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQGNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVISCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            9349..10182
                     /gene="fdhD"
                     /locus_tag="ACHOUK_003977"
     CDS             9349..10182
                     /gene="fdhD"
                     /locus_tag="ACHOUK_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFP9899326.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2603 a         2446 c         2602 g         2598 t
ORIGIN      
        1 aacattatgc aaagataatg ggctggcatc attgtcagta tcattcattt actgctctgg
       61 atgttataaa taattatgta gcatgtttgc tcttaaaccg tgggaaggag gctgtaatgg
      121 ttgccaatat taatctcatt aaacaagaaa gttattctgt tgttaattta gaaaaacaac
      181 taatgagtaa tgaatcaccg aagcaatcgc tattaaatga tttttcacat cgccttaaaa
      241 tggaaggtga ggaacaactg ttccattgtg ttaacgaact gaagtatggt ttgtcgagta
      301 ataatgaata tgacctgttt aaaaatagag aaacctcact gtggaaacgc aaagtaccag
      361 aaagcgcagc gctggcaatg acgggaacag tatataaatt atttggtagt cgcttactca
      421 acgcccgcaa tcagttagat aaaaatctcc tcgaaatggc tatgcaaatg gtttatggca
      481 aagatatggt gacaaagtcc ggggatatat tgattgatat acaattaaat aaggatggca
      541 gcctccagag taagcactac acatttgatg taggtgaagt tataaacagt tataatattg
      601 acctctttgt gaatgcttcg agtattaatc aaacttactt acatgataaa aatcctggtg
      661 atgttattag cctgtatggc actttagata acattcctct tgtagttaag gaatgcacgg
      721 gtaaaaatca tcaaattaac gttaaagcca aattgccaca agaacgaagt gaaaaagttg
      781 aggatatgtg tgaaagaatg agaggtggca tctctatgtt taatcacacc acaaaaacag
      841 ctggtaatat agagcataat ttgcagctaa gttttctcgt ggattcacgg ccgtcgatca
      901 ccaataaata ctcagccaaa gatgtagcgc cagacatact tgtattgccc gtcaacgtat
      961 atcatgcgga ttttaaaatt aaaatcaata atgcagtaga gaagacttag tcaattgtgt
     1021 tatgtgctgt gcaaaaaaat ctccatgcag caaggaaggt tcttagcggg ataataggta
     1081 atgtaccaaa aaataataaa ccaaaaacaa tcatttttga tttacgtttt cttagtgcaa
     1141 ttaagcagaa agtttttttg ggcagtgaac aaccgttgtt tgtaaagcat accgcaatga
     1201 tgagggaggc atgtaaggaa ttaccgcagc tagtggagta tgttccttta gcctgtgaag
     1261 cgcatagcca tcgcagcgtc gcccttgcca aaaccgtggc tgcacccatc atggcgacag
     1321 gtgtgggcgc ggctttaatg tatgcaacat catcaggacc gtattatgcg caaagtcttt
     1381 ctggttcgcc ggatatcgag atgataaaaa aacgcatggc tgaattgttt aaccagtcgg
     1441 atacttctgt gcgcaacaaa tatcgcccgg cttttgataa atggaataaa ctggtagata
     1501 aattaaatca cgaaagaaat acagttccgt ttgcgtgttt gaccaccatt atagcgacag
     1561 cgataaatga aactcctgag ggggatacga atgttgccgt tgtgatgggg tgtaaatctg
     1621 cgaaagatcg cacgatatcc attgtccttg gcaactcgat gttgcaaaca ctctttgaaa
     1681 aacgcctcgc cgatgatgga gaaatagaaa acctttttga tcaacaggga tattttagtt
     1741 gcgattcatt aacagccgaa gaagtaatga tgttaaaaga cctttttgat attcgagttc
     1801 ttcatctctc taacaaattt aatgttgggt tgcaaggtaa tatcaacacg gatgttttac
     1861 aagattcgtt ttttaaaaat gtcgatttca ttcatgaatc taactcgtat accgtaggga
     1921 tgggagtctg aagagtaaaa ctcattagga ttatcagaat tcggccaatg cattgcaaaa
     1981 atgaaataca aagagagatg tgtgcaggcg cttagccgcg aggggcatgg cgaagccata
     2041 cagaaagcac cggcattcag aactcctgtg aaagcggcat agtgccgctg ctcaggagtt
     2101 ttgttatgac ccgtatcgtg ctgaacgttg ccaccctatg atgcgattaa aaaaactcac
     2161 cgtcgagccg atgattgacg tcaatgctat cggatgatgc cgggggtgtt ccggaaggaa
     2221 ttccttgttt cccgaactgt taccaacaaa attataacga tgagcaagta aagaccggtt
     2281 ggtggcagta ctgaccggtc tgtgatggaa gattaatttc accttaaatc ctggtgctgt
     2341 taggatgctg acgtttttcg ggtacaccat tcagattatg caaataaaga ttcaatgaaa
     2401 aaataaatat ccctgaatat aatatattcg tagcacccat aactcatcct gggcgataac
     2461 taaaccattt ttgagtaaca aatgaaacaa ctgcaacgct cttattattt ctggaaaacg
     2521 tctcgcaaaa acaaaacaac atgcaaaaat ccgctttcgt taattaattt ttcggcgtaa
     2581 ttttcgcaca tacattaatg agtaatatat gtaaggtggt ggtaatggcg atgaatacgg
     2641 tttttcttca tttatcagaa gaggcaatta aacggctgaa caagcttcgg ggctggcgta
     2701 aggtttcacg atctgcaatt ttacgcgaag cggtagagca atatctggaa cgtcagcaat
     2761 tcccggtgcg taaagcaaag ggaggcaggc aaaaggggga ggtagttggt gttgatgatc
     2821 agtgtaagga gcataaggaa tgacaaaaat ttttcattct tgaatataaa aaacagatgc
     2881 ccttattctg gtattaatag aaggctgttt tacttgaact tataataact gcaactgtta
     2941 catcatatct ggaaaacgcc tcgcaaaaat aaaaaatgat gcgtaaatga gctttcttta
     3001 aagattcgac aagggtactt ttctctccat agatatcaaa tgatattggt caaatattta
     3061 tgaactctct ggcaggtatt gatatgggca gaattatact cgatttatcg aatgaggtga
     3121 ttaagcaact tgatgatctt gaggtgcagc gtaatcttcc tcgtacggag ctattacggg
     3181 aagcggtaga tcaatacttg gtaaatcaat cgcaaacagt aagaaccagt gcttttggca
     3241 tatggcaagg gtgtgaggaa gatggtgtcg aataccagcg taagctgcgc gaggaatggt
     3301 aatgggaaaa aatgatatca atcagattgc tgacaacgtg cgcgttgttc atgccggatg
     3361 cggcgtgaac gccttacccg gcctacaaag tcgtataaat tcaatgtatt gcagtttatt
     3421 ggtaggccta ataagcgcgg cgcatcaggc aatcttacgc ttatcatcag tctcataccc
     3481 ataaccttat tgatgtgggg gtgggtaaat tgaggcaaaa caggcactgg cggcatagct
     3541 tgaggtggta acgcggaata cgagagatgg tgtcgtcaca ccctatgagc tgtaatccgg
     3601 gcgaccagcg ccacccggaa aacgtcagag attactcccc ttcacccgga aacaggaacg
     3661 ggttgatgga actgcgggca tagccttctt gctccattcg cgcgtccagt accagagagg
     3721 cgaggtcatc tgccacggct tcaactttcg gttctttttc ctgatagaga attttcaggt
     3781 aagtgccgca gtcatcgcag ctttcggctt taatcgcggc ctgttcgtca tccagcgacc
     3841 agtaatgcag tttgccgctc tgctcgcagt tgctgcattt tacgcgcaca acgtgccatt
     3901 cagtttcaca caggttgcag tgcaggtaac gtagcccctg agtggtgcca atttgcacca
     3961 tgctggacac cggcatagaa ccacaaaccg ggcaatattg acgttgctcg ccgtattcag
     4021 cgcgggcttt gccggggatc agattggcca tctgcgccca gtagagcgac agtgcagccc
     4081 agataaacgg cgctttatcg ctgctgacgg acgagaaatc agaggcaaac agtgcgctgg
     4141 ccatatcttc cagctcctga gtcgatgcct tctccagatt ctcaatcact gccagcgccg
     4201 ggccgctcat ttcaggtttc agctcagcaa tcagcgccat cagcagcttt tgccagtgct
     4261 tatcacgcgg cagaacgtga atatccagcg ggggcttgcc ttgtgcgctg gcttctttaa
     4321 tgcgcgcagt cagatccatc tccagcggat ggtcgtacag caccacttcc tgggcgtggg
     4381 cgataagcgc agcaaagcgc aggtaatcac ccagcggatt attttctgcc agctcgcgca
     4441 gacgctcggc gcggcggttg tataaattct tgagccgagg gaacaataac ggcggaatca
     4501 tatccgccgt acgtttctcg ctcgaaccca gctcatcttg cgggattatg cgaatactca
     4561 ttcagctttc ttttccgttg tcttgcggac ctcacggtac cagcgcgggt gatgtttctt
     4621 cgcccaagcg ctggtaaccc atccttccac catggcggta atcgtgcctt tcacccaaag
     4681 ggcggcgtag atatgcacca tgataaccac aattaacgcc actgcggcaa atgaatgcag
     4741 cattaacgcg aatcggatca ccgggattga gaaagcaggc gcaaaataag gacgccagat
     4801 aatcacgccg ctcaccagca gcagaaccag gaaaataatc gccgcccaga aaacgcattt
     4861 ctgaccgaag ttataacgcc cggtgtcacc tacttcctcg ttgacgacga tcttacgaat
     4921 attcttcgcc caaaagatat catcccgatt gattaggttg tgatgccagt aacggaaaaa
     4981 catgatgatg aacgaggcaa acataaccac gccgacaaac gggtgcagaa ttcgcgccag
     5041 ctgcggtgtg cccatgattt gcatcaacca gttgaaggac gggaacaaaa agcccagccc
     5101 gctcaccgcc gccaggatga agcagaaggc ggtgatccag tggttgatac gttccggcgc
     5161 ggtgtagcgc acgatggtgt cacgtcgttt catttgcgct cctcgtcttt ctcttcgtgc
     5221 agattattct cttcctcatc cgcatggttc ggaccgacac ccacgtagtg gaagatactg
     5281 gctgcgaagg tagccgcaaa gccaacagct gcgagcggtt tccagatgcc tttccagaat
     5341 ttcacggttt cgctgatttc cgggttctcc ggcaagccat gatacagatt tggcttgtca
     5401 gcatggtgca gcacgtacat aacgtgtgta ccaccgacgc ctgccggatc gtacaggccc
     5461 gcattgtcgt aaccgcgggt tttcagctcc gccacgcgct cgctcgccag cgttttcatc
     5521 gactctttcg taccgaagtg aatcgcgccc gttgggcagg tcttcacgca ggctggttct
     5581 tgcccaacca ccacgcggtc aacgcacagc gtacatttgt agacgcggtt gtcttccggg
     5641 ttgaggcgcg gaatgtcgaa cggacagccc gcaatgcaat aaccgcagcc aatgcactgc
     5701 tcggactgga agtcgacgat accgttggca tactgaataa ttgcaccttc cgccgggcac
     5761 gctttcagac agcctggatc ggaacagtgc atacagccgt ctttgcggat cagccattcc
     5821 agtttgtcgt tctgctccac ttccgagaag cgcatcaccg tccacgattt ggcgcttaaa
     5881 tcattggggt tgtcgtacac cccaatgtta ttgccgacgg tatcgcgaat gtcgttccac
     5941 tctgaacacg ccacctgaca ggctttacag ccgatacagg tggtgacgtc gatgagtttc
     6001 gccacttctt cctggaagtc ccgcgcctga ggcgcggggg tcagaccgtt agtcgcggaa
     6061 cgacgaatga tatcttgaga ttgataagcc atatgtcgtc tccgttacac cttttccaca
     6121 ttcacaagga aggacttaaa ctccggcgtc tgcgtgttcg catcaccgac gaatggcgtc
     6181 aacgtattgg caataaagcc ttttttcgca acaccttcat agccccagtg aataggaata
     6241 ccgatggtat cgatatcttt gccgttcgct ttcagcgtgc gaatacgttt ggtcaccacc
     6301 gctttggctt tgatatagcc acggttggag gagactttca cggtatcgcc ctgggcaatg
     6361 ccaagtttat tcgccagcga ctccccgatt tccacaaact gctctggttg caaaatcgcg
     6421 ttcaacagcg cgtgtttggt ccagtagtgg aagtgctcgg tcagacgata ggtggttccg
     6481 acatacggga acttatcggc tttacccaat gcttcggcgt cgtctttaaa gatacgtgca
     6541 gccgggttcg agataacgtt tggatgcagc gggttagttc ccagcggcgt ttcaaacggc
     6601 tcgtagtgtt ccgggaacgg accttccgcc atcttatcga gggcaaacag acgccccatg
     6661 ccttcctgct gcatgataaa cggcccgacg ccgctacccg gaggcgctgc gctgtagtcc
     6721 ggaatatccc agccggtcca cttagtgccg tcccatttca gcaactgacg cttcggatcc
     6781 cacgggttac cctgcggatc tgcggaggcg cggttataca gaatgcggcg gttaagcggc
     6841 catgcccatg cccaacccag cgtgttaccg aggccagacg gatcggcgtt atcacggcgt
     6901 gccatctggt tgccttccgg cgtccagcta ccggcgaaaa tccagcaacc acaggaagtt
     6961 gtaccgtcat cgcgcagttg ggcaaacgag ctaagttgtt ggcctttctt gacgataacc
     7021 gcaccggttg ccggatcggt aatatcggcc agcgccttac cgttgctctc catcgctact
     7081 tcttccgact tcggctcatg cggaatggcg tagttccagg tcatgttcag cacctggtcc
     7141 gggttcgcgc caccctgttc ggcatacatc ttgcgcaagc gcaggaagat accggagagg
     7201 atctcgccat cagtcagcgc aatccccggg gcgtccgcac ctttccagtg ccactgcaac
     7261 cagcgacctg agttaacgat tgaaccgttc tcttctgcga agcaggtcga tggcagacgg
     7321 aacacttcgg tctggatctt cgacgagtca acttcgttca gctcaccgtg gttctgccag
     7381 aagttagagg tttcagtgtt cagcgggtcg atggtgacga ggaacttcag tttcgacaga
     7441 caactgatca ctttgttttt gttcgggaat gaggcaacag ggttaaagcc ctggcagata
     7501 tagccattga ccttgccttc tttcatcatc tcgaagtatt gcaggacgtc gtagccttta
     7561 tcccacttcg gcaaccagtc aaagccccag ctattttctg ccgtcgcttt atcaccaaag
     7621 aaggccttca tcatagagac gaagaatttc gggtagttgc cccagtagtt tccctggcct
     7681 tccagcagcg gttttggcgt gttggcggta aggtaggttt gcagatcggt ctgcttctcg
     7741 cttggcagcg tcatgtaacc tggcaggctc tgcgacagca gccccaggtc cgtcagcccc
     7801 tgaatattgg agtgaccgcg cagggcgtta acgccgccgc cagccatccc catgttaccg
     7861 agcagcagct ggatcatcgc catcgtacga atgttttgcg caccaacgga gtgttgcgtc
     7921 cagccgaggg catacaggaa cgaggcagtt ttatcgtgag cactggtttc tgcgatgtat
     7981 tcgcagactt tcaggaacgc gtcttttggc gtaccacaga tgttttcaac cacatctggc
     8041 gtgtagcggg aaacgtgctg tttcagcaag ttccacacgc agcgcggatg ttgcagcgtg
     8101 gtatcgcgtt tggcgaagcc gttttcgtcc agttcataag tccaggagga tttatcgtac
     8161 ttgcgttttt ccgcgtcgta gccggtgaac aggccatctt caaagccgta atcctcacgc
     8221 acgatcaggc tggcgttggt ataggcttca gtgtattcgc ggttgaattt ttcattgttc
     8281 agcaggtaca gcaatacgcc tgacaggaaa gcaatgtcag taccggaacg aataggggca
     8341 tagtagtcag ccaccgcagc cgtacgcgta aagcgaggat cgatcacaat cagcttcgcg
     8401 ccattgtgaa ttttggcttc catcgcccag cggaacccga ccgggtgagc ttcagcggcg
     8461 ttaccgccca tcaccacgac gaggttggcg ttcttgatgt cgacccagtg gttggtcatc
     8521 gcaccgcgac caaatgttgg agcaagactt gctaccgttg gtccgtgtca gacacgcgcc
     8581 tggttgtcga ccgcgagcat acccagcgcg cgggagaatt tttgcgttaa atagccggtt
     8641 tcgttactcg acgcggaagc acacagcatc ccggtggaga gccagcggtt aacagtcacg
     8701 ccttcggcgt tttgcgcaat gtagttagca tcgcggtctt ctttcatcag tttggcgatg
     8761 cgatcaaacg cctcttccca actgatttgc tgccatttat cagaacctgg cgcacggtat
     8821 tccggaaact tcagacggct ttcggagtgg atgaaatcca ccaggccagc gcctttcgga
     8881 caaagtgcac cgcggttgac cgggtgatcc ggatcgcctt cgatatggaa gatagatgct
     8941 ttggcgtttt ttgctccgtc accgaggctg tacatcaaca gcccacagcc tacggaacaa
     9001 taggtgcagg tattacgggt ttcgcgggtg cgcagcagtt tatactgccg ggtttccgcg
     9061 agtgctacgc tgggtgcaaa acccagtgcc gctgccgtgg tgcctgccat accgccagcg
     9121 cagatcttaa agaactgcct tctgctgacc tgcatggatt gctccttgtt tcgacattgt
     9181 cacgtcccat ttacattcgc ttgctgcgtg tgcagggagt gggagttatt tttctttgcg
     9241 gaaggggccg caaaggtcca gaattggctc aatttccctc catccaggaa gggtttgtaa
     9301 cagaatacca taatgttggt gtgtgtgttc ttatctggtt aagagaaagt gaaaaaaaca
     9361 cagcaaaaag aaatcgaaaa tgtgacaaat atcacaggtg ttcgtcaaat tgagttatgg
     9421 cggcgtgatg atttacaaca cccacggtta gatgaggtcg cggaagaagt tcccgttgcg
     9481 ctggtctaca acggcatttc gcatgtggtg atgatggcgt cgcccaaaga ccttgagtac
     9541 tttgcgctcg gtttttcgct ttccgagggg ataatagaaa gtccgcgcga tatcttcggc
     9601 atggatgtcg ttccttcctg taatggtctt gaagtacaaa ttgagctttc cagccgccgc
     9661 tttatggggt tgaaggagcg ccgccgggcg ctggcgggac gtacgggctg tggcgtatgc
     9721 ggcgtggagc aacttaatga catcggaaaa ccggtgcagc cgctaccgtt cacccagacg
     9781 tttgatctca acaaactgga tgatgcatta cgtcatctca acgattttca gccagtggga
     9841 caactgaccg gttgtactca cgccgccgcc tggatgttgc catctggcga actggtcggc
     9901 gggcatgaag acgtgggtcg ccatgtggcg ctggacaaac tgttaggccg ccggtcacaa
     9961 gaaggggaaa gctggcagca aggtgcggta ctggtttcca gtcgtgccag ttatgaaatg
    10021 gtgcaaaagt cggcgatgtg cggcgtagag attttgtttg cggtgtctgc cgcgaccacg
    10081 cttgctgtag aagtggctga gcgctgtaat ctgacactgg taggtttttg taaaccgggt
    10141 cgggcaacgg tttataccca tccgcagcgt ttgagcaatt aattaaaata agtcctgcga
    10201 tatatattaa attgcaggac ttattcattt cgtgaatttt attatttta
//
LOCUS       DAZKIP010000139        10130 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZKIP010000139 DAZKIP010000000
VERSION     DAZKIP010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.140"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            157..1206
                     /gene="allD"
                     /locus_tag="ACHOUK_003978"
     CDS             157..1206
                     /gene="allD"
                     /locus_tag="ACHOUK_003978"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFP9899327.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHVVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRQAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            1228..2463
                     /gene="allC"
                     /locus_tag="ACHOUK_003979"
     CDS             1228..2463
                     /gene="allC"
                     /locus_tag="ACHOUK_003979"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFP9899328.1"
                     /translation="MITHFRQTIEEALPWLSSFGADPAGGMTRLLYSPEWLETQQQLK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVILSGSHIDTVVNGGNLDGQFGALAARL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPDAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIRIDIDLWMDEEPVPMNKDL
                     VATLTELCESEKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            2474..3259
                     /gene="allE"
                     /locus_tag="ACHOUK_003980"
     CDS             2474..3259
                     /gene="allE"
                     /locus_tag="ACHOUK_003980"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFP9899329.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFS
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGYAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(3487..4632)
                     /gene="glxK"
                     /locus_tag="ACHOUK_003981"
     CDS             complement(3487..4632)
                     /gene="glxK"
                     /locus_tag="ACHOUK_003981"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HFP9899330.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGITGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LVANGGNLARVASIEMEECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLRAV
                     NLEQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(4654..5955)
                     /locus_tag="ACHOUK_003982"
     CDS             complement(4654..5955)
                     /locus_tag="ACHOUK_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFP9899331.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(6012..7373)
                     /gene="allB"
                     /locus_tag="ACHOUK_003983"
                     /pseudo
     CDS             complement(6012..7373)
                     /gene="allB"
                     /locus_tag="ACHOUK_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
     gene            complement(7410..8801)
                     /locus_tag="ACHOUK_003984"
     CDS             complement(7410..8801)
                     /locus_tag="ACHOUK_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HFP9899332.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGLGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPLMEPLS
                     RVSWFVGVIVAFA"
     gene            complement(8970..9848)
                     /gene="glxR"
                     /locus_tag="ACHOUK_003985"
     CDS             complement(8970..9848)
                     /gene="glxR"
                     /locus_tag="ACHOUK_003985"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFP9899333.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     NVDTARQVTEAADIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(9949..>10130)
                     /locus_tag="ACHOUK_003986"
     CDS             complement(9949..>10130)
                     /locus_tag="ACHOUK_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFP9899334.1"
                     /translation="IGHLQIADNPHRGEPGTGEINYDYLFKVIENSDYNGWVGCEYKP
                     QTTTEAGLRWMDPYR"
BASE COUNT         2700 a         2664 c         2410 g         2356 t
ORIGIN      
        1 ataagcgtgg ttgttttaaa aaccaggtta aaagcgagcg gattatcaaa taaattctaa
       61 ctatattttt ttgcctgtct ggatcacata atccagatat tttccctgta tgttaattgc
      121 agtcatgctt cacaccgtcg ttaaaaagga agacagatga aaatcagtcg ggaaacactc
      181 caccagctaa ttgagaataa actctgccag gctgggttaa aacgtgagca cgctgcaacc
      241 gtggctgaag tattggttta tgccgatgcc agagggatcc actctcatgg cgcggtgcgc
      301 gtggaatact acgcggaacg catttcaaaa ggcggcacca accgcgaacc ggagtttcgt
      361 cttgaggaaa ccgggccgtg ctcggcaatt ttacatgccg acaatgccgc cggacaggtc
      421 gcggcgaaaa tgggtatgga acatgccatc aaaaccgccc agcaaaatgg cgttgcggtg
      481 gtcggtatca gccggatggg tcacagcggc gcaatctctt attttgtgca gcaggcagcc
      541 cgcgccggat taattggcat ttcgatgtgc cagtccgatc cgatggtggt gccgtttggc
      601 ggcgcggaaa tttactacgg tactaaccca ctggcctttg ccgcgccggg agaaggcgac
      661 gagatcctta cctttgatat ggcgactacc gtacaggcat ggggaaaagt cctcgacgcc
      721 cgctcgcgta atatgtctat cccggatacc tgggcggtcg ataaaaacgg tgcaccaaca
      781 accgatccgt tcgcggtaca tgctctgctc cccgcagccg ggccgaaagg gtatggcctg
      841 atgatgatga ttgacgtcct ctcaggcgtc ttactcggct taccgttcgg gcgacaggtt
      901 agttcgatgt atgacgattt acacgccggg cgtaatttgg ggcaattaca tgtagttatt
      961 aacccgaact ttttctcctc cagcgaatta ttccgtcaac atcttagcca gaccatgcgc
     1021 gaattaaatg ccattacccc cgcgcccggt tttaatcagg tttattatcc cggacaggat
     1081 caggatatta aacaacgcca agccgccgtc gaaggcatcg aaattgttga tgatatttac
     1141 cagtatttaa tttccgacgc gctttataac acgtcatacg aaacgaaaaa tccctttgcg
     1201 caataattat tgagacagga ctttcttatg attacacatt ttcgtcaaac catagaagaa
     1261 gcgctgccct ggctttcctc ttttggcgct gacccagcgg gtgggatgac ccgtttactt
     1321 tattcgccgg aatggctgga aacccagcag caattaaaaa aaagaatggc cgcaagtggg
     1381 ctggaaacac gtttcgatga agtggggaat ttatacggtc gcctgagtgg caccgaatat
     1441 ccacaggaag tgattctgag cggttcgcat atcgataccg tggttaacgg cggtaacctc
     1501 gacgggcaat tcggcgcgct ggcggcgcgg ctggcaattg actggctgaa aactcaatac
     1561 ggcgcaccgc tgcgtacggt tgaagtggtg gcgatggcgg aagaagaagg tagccgcttc
     1621 ccgtatgttt tctggggcag taaaaatatc tttgggctgg cgaatcctga cgacgtgcgg
     1681 aatatctgtg atgccaaagg aaatagtttt gtcgatgcga tgaaggcttg cggatttact
     1741 ctgccggacg ccccgctaac tccgcgtcag gatattaaag cctttgtcga actgcatatc
     1801 gaacagggct gtgtgctgga aagtaatggg caatcaattg gcgtggtgaa tgcaattgtc
     1861 gggcaacgtc gctatacggt gacgctgaac ggcgaatcaa accatgcagg caccacgccg
     1921 atgggttatc gtcgtgatac ggtttacgct ttcagtcgca tttgccatca gtcgatcgaa
     1981 aaagcgaaaa agatgggcga tccgctggtt ctgacctttg ggaaagtaga gccgcgcccg
     2041 aatacggtga atgtggtgcc gggtaaaacc acgttcacca ttgattgtcg tcataccgac
     2101 gccgccgtgc tgcgtgattt cacccaacag ttagaaaacg acatgcgggc gatttgcgat
     2161 gaaatggaca ttcgtattga tatcgattta tggatggacg aagaacccgt gccgatgaat
     2221 aaggacctgg tcgccaccct gacagaattg tgtgaaagtg aaaaactgaa ttaccgggtg
     2281 atgcacagtg gtgccgggca cgacgcgcaa attttcgcgc ctcgcgtacc aacctgcatg
     2341 atttttatcc ccagcatcaa cgggatcagc cataacccgg cggaacgcac caatattacc
     2401 gaccttgccg aaggggtcaa aacgttggca ctcatgcttt atcaacttgc ctggcagaaa
     2461 taaggagtca taaatgggat atttaaataa cgtcaccggt taccgcgatg atttactggc
     2521 taaccgtgcg attgtgaaac acggtaattt cgcactgtta acaccagacg gtctggtaaa
     2581 aaatattatt ccgggctttg aaaattgtga cgcgacaatc ctctccacgc caaagctggg
     2641 tgcctctttt gttgattatc tggtcacact gcatcaaaac ggcggcaacc aacagggctt
     2701 cggtggcgaa ggcattgaaa cgttcctgta tgtgatctct ggaaatatca ctgccaaagc
     2761 cgaaggcaaa acattttcct taagcgaagg tggctatctt tattgcccgc ctggctcctt
     2821 aatgacgttt gttaacgccc aggccgaaga cagccaaatc tttttatata agcgccgcta
     2881 tgttccggta gaaggctatg caccgtggct ggtttctggc aatgccagcg aactggaacg
     2941 cattcactat gaaggtatgg atgatgtgat cctgctggat ttcctgccca aagagttagg
     3001 ttttgatatg aacatgcata tcctttcttt tgcaccaggt gccagccacg gttatatcga
     3061 aacacatgtt caggaacacg gtgcctatat tctttccggt cagggggttt ataacctcga
     3121 caataactgg atcccggtga aaaaaggcga ttacatcttt atgggcgctt attctttaca
     3181 ggctggttat ggcgtagggc gtggtgaagc gttcagctat atttactcga aagattgtaa
     3241 ccgcgacgta gagatttaag tcataccact cgcctgatta tttttagcta tgttggattt
     3301 tgccacggtt tataccgtgg ctttttttgc aacatagcct gatgcgcttc gcttatcagg
     3361 cctacatgat ctctgcaata tatagaattt gcgcgatttt caggaaggat atggtactca
     3421 tgccgcatcc ggcatgagta ctgcactctt atgcgatata aatcgcatcc gctttaaagg
     3481 taagggtcag tttttaattc cctgacctat tttaatggcg caggcaatat ttcgcgcgct
     3541 gttgaagaga ttggtttcac cgctggcgag cacttcggct aaaggtgcca gacgtggcaa
     3601 aatgctgaat accgcatcaa tgccgtactg gtgcaccact tccacgccat cacccaatac
     3661 gccagcaatc ccaatcaccg gtacattaaa ctgcttcgcc accgacgcaa cgcccagcgg
     3721 cgctttaccg cctgccgttt gcgagtcaat gcgcccttcc ccggtgatca ccagtgctgc
     3781 cccctgcacc gcctgctcca ggttgaccgc ccgcagtaca atctcaatac ccggtttaat
     3841 atccgcattg aggaataccg ccgccgcaat ccccataccg cccgcagcgc caccgcccgc
     3901 catctggcag acattaattt cagtttgctg ttgtaaaaca cgggcgtaat tttgcagccc
     3961 ctgttcaagt tcttcgacca tctccggcgt tgcccctttt tgcgggccaa acaccgccgc
     4021 cgcgccgcgt gcccctacca gcgggttatc aacgtcacat gctacttcaa tatggcagtt
     4081 cgccagacgc ggatcgcatt cctccatctc aatgcttgcc acgcgcgcta aattgccgcc
     4141 gttagccacc agcacctgac cgtcggcatc aaggaaacgc acaccgagcg cctgcgccat
     4201 gcccataccg ccgtcgaccg tcgcactgcc gccaatgccg agaataatat ggcgaatgcc
     4261 gttatccagc gcatgacgaa ttaactcccc cgtaccaaaa ctggaggcca gcaacggatt
     4321 acgcttttca ggcgcgacca gcatcaggcc acttgctgcc gccatctcaa tcaccgccgt
     4381 tttcccgtcg ccggtaatgc cataaaaagc attcactttt tcgcccatcg gcccgcagac
     4441 ttcaagcgtc acgatgttgc cgcccgtcgc ggcgaccatc gcatccaccg tcccttcgcc
     4501 gccatccgct atcggcaaac agatatagtt cgcatcggga aagagggtcg aaaacccggc
     4561 tttaattgcc tgacaacatt tttctgcact caagctctct ttaaaagagt ctggcgcaat
     4621 gacaatcttc attatcaatc ctttttcaac aaattattcg atgcgttccc acggaataag
     4681 gttatccata agaacagcca gtaaaatccc gaccaataag ccgttactga gcagaggacg
     4741 aagcgttaat ggaaggtctt gcagatacac aggcggtaat gccattaaaa atatgccgac
     4801 aaataacggc aatgcgagtc gataaatatt gcgagcggta aacgttattt gctggctaaa
     4861 caccagcgcg gaaaagagta aaggtagata agaaaccagc atgaccgcac tactcacggg
     4921 taaagggata ctgcaaaaca gtcgcgtgag tgccggaacc agcgccacca gcaggcaaat
     4981 aacgctgcca taaataaacg aacgccgcgt gtaatcgcca gtctgggtta ataaaccaat
     5041 ggatgaaaca aacggtgaaa atggaattac tgccagcggc acggttatca gtgtcataaa
     5101 tccggtcgcc acaaagctac gacgataacg cgtattcccc gcgccctgct gcggataaaa
     5161 aacatccgtg ccccgaatcg caccgtaggt attgctgata ttcaccagac ctgtaatcac
     5221 cgccgtcaga ataatccccg gcgacaaagc accgccactg ccgagcggga accactgcca
     5281 gtgcaactca ccggagagcg aatgcgaaga aggaaagcaa aagtaccaca acaaccagcc
     5341 ggttatggtg ccgaccagca ggccataacg ggcaaaacgt tgcggcagaa agataatcat
     5401 cgccagtacc aggcacatca ccgccaccga gagcgcgaac ggcggtaact gaattttaaa
     5461 attcgggtcg gctatgccaa acggcagccc gagcatacct ttgaaaaaga tagtggtcag
     5521 ctgcgcgccc agcatcaaca taaacaagac catcaccgac ggcgtaaata accgcgccag
     5581 gcgatgacct aatccgctaa aaccaatcaa catcgtcagc acgccggaga gtgcaatccc
     5641 caccgccagg ctggtagcga tatcgttgat cggtgtcccg cgcgatgctt cgccaagggt
     5701 gatagttagg atggttcccc accacaggcc ccccggccct tccataatag cgcgacgatg
     5761 accgcaaaac gcctgagcga agcaggccag tgcggtagca agaaaagcat attgcgtgag
     5821 cgtaagcagg ctactttgcg gcaactgaaa agcagaaagt agcgtaggag gaaccacaac
     5881 cgtgttgcaa aaaataaaga aaaaccactg aaatcctgat aacaggcttt cgcggctaac
     5941 tgcaaaattg aacataaaca caccttaacc ggagaatgcc cgccgcaagg acgggcattg
     6001 caggggccag attactgctg atgtttaagg ataaattgac ctttcggcgc aacagggaag
     6061 ccctgttcga tgtcgtaaat cacatcacca cgtaagatgg ttttcgtgat acgcgcgcca
     6121 atggtacggc caacatacgg gctgactttg tggcgatatt ccagatcgtc attggtaaga
     6181 acatagctgc tattcggctg aatgaagacg aagtcggcat cttttcctgg ggcgatacgg
     6241 cctttttgct gcagaccaaa aatatctgct gcgttagtcg ccattaattt gccgaacatt
     6301 ggcagagaca ttccgcgttt ctgtaccgct tcatcgaaca tcacgtccat acagttttgc
     6361 agaccggcaa taccgcccca tgcttccatg atgttgccgg ctttcatttc cggagggcat
     6421 ggtgagtggt cggaaaccag gcggtcgatt tcaccgttaa acagtttttc ccacatgcct
     6481 ttctagtttt ccagatcgcg gatcggcggt gaacacttcg ccagagtacc gatttcttcg
     6541 aactgatcgg tatccagtac gaagtaatgc gggcaggatt cacaagtaac gtcctgacct
     6601 tcctgacgtg cacgagtcac ttcttcaacg ccttccgggc tgctgatatg gcaaacgtgc
     6661 agacggcaac cggcaacttt cgccagatac agcacgcggc gaatcgcttc cacttcggta
     6721 aataccggac gcgaagccac atagtcatgg gcagttacgc gaccttcacg tttcgcttct
     6781 tcgcccagtg cgtcacagat cagcgcgttt tcgcagtgca ccagcaccgg ctgccccagt
     6841 tcgcccagct tctgcgcacc tttgaagaac tgccagtcgt ttacatcacg gaagtcgttg
     6901 tcgataccgc gatcgccaca ggtcgcaacg aagcatttga agccgacaac gcccacttca
     6961 tccagctcat gcagacggtc gatgttgtaa gacaccaggc caccgagttg tgccgcatcg
     7021 atagtcagct tgcctttagc ggcatcgaac ttcagttcaa ttgaagcgcg gtcaaccgtt
     7081 gcaggcagct ggttgagcgg catttcgatc atggtggtga taccaccttt tgccgctgcg
     7141 cgagtaccgg tttcataacc ttcccagtgg ctacgacccg gttcagaaat atgggtgtgc
     7201 gcatcaacca tgcccggcga aaccaccaga ccagacgcat ccataacttc ttttgcatcg
     7261 cccagatcct gaccgatagc agcaattttg ccgcctttaa cggcgatatc tacaacgcga
     7321 gcttcgtttt ctaaaataac ggtgccgttt ttaatgatta aatcaaaaga cataacaaac
     7381 tccttgtaaa atacagattg gtttttaact caagcaaagg cgacgatgac gccgacaaac
     7441 catgaaacac gcgataacgg ttccattaag ggaataaact taccgccaag agataaaata
     7501 acggccacca gagttactga aaacgcggtg aggttaaaac cgttatcgta atatttataa
     7561 tcgccaggtg cggtgtacag ttcatcaaga ttaatttgcc cacgcatcac cacaaaataa
     7621 tgcgccatca tgacaccaat taccggacca agcattccgc cgataatatc gaggaacaga
     7681 taaatactgt cctgattttc cattaatttc cacgggcaga tcagcaggct gataatactg
     7741 gcaatcagta cgccgttttt ataggtcagt tttgtcggtg caatggcagc aatctgataa
     7801 ccagccggaa taatattacc agtggcgtta gtggagatag tcgtcatcag aataaccagt
     7861 accgcaaaga acgaggcaaa caggctgtcc caacgctgaa caatatccag cacgttccag
     7921 gtatcagcgc cgtaatgaat actggctccg gcaataatac atacccccgc gaccgcaaac
     7981 agaatatagg ccacaactaa acccagcgtt tgccccagcg cttgctcacg aaacgagtgg
     8041 gcgttttgcg taaagtcgga tgcgctcacc gccggtgccg cccagaccgc aactaccgcg
     8101 ttaatcacca ccaggaacaa gaatccactg ttttctgctt tctgaatacc gctcggaatg
     8161 tagtcaaaga ttggaccgag cccgaccagc gaaatcgccc aaatcgccat accgccgaaa
     8221 acgatataga tgcacgggtt aagaatggca gtgaatttat ttaaaacttt gccaccgcca
     8281 aaacctatac caacgttgac cagccagaag agtaagaaag taattaagcc cggtagagaa
     8341 aggcctaaca gagtgaaatc accaccgaga gtcaaaaatc ccggccagat tttgccaatc
     8401 agaatcaagc aggccagtga ccccgcgtaa cattgcaggc caaaccacat aatggcagca
     8461 ataccgcctc ttaataatcc gggaaacagt gcgccacgta cgccgtaaga agcacgcagg
     8521 atcatggcaa acggtacgcc gtatttactg cccgcagcgc cgtttaatac cattaccgcg
     8581 gcaatgaaaa aggcgctgag gataattgcc agcataatac taaaggtaga caagccgaga
     8641 ataaaaaagc cgccgaccat cacataattg ggaacgttat gaaccgaacc catccataag
     8701 gtaaaatagt taaatgtttt ccaggtccgc tggctttgcg ttttcggcaa tagatcttcg
     8761 ctatagccgc gttgctggaa tagttttctc tgatgttcca taaatccctc ttaatttcac
     8821 gtatcgaata acgttgtttt aatctagcta tgaggaaaaa ataaaataaa aatattattt
     8881 ttaatactga agcaacatct ttggctgctt tcttttttaa tattgctggc cgattcattt
     8941 tttgatatcg gccattttta ttgcgggtat caggccagtt tatggttagc cattaattcc
     9001 agcgcctgca ctaacgcaga gtgatccaac tggctgccac cgtttgccgc gcaggtatta
     9061 aataactcct ggcaggtcgc agtgtttggc aggttcagag ccagtgcctt cgcactttgc
     9121 agtgccaggt tgagatcttt ctggtgcagc gcaattttga aacctggatt aaaggtgcgt
     9181 ttaatcatgc gctcaccgtg aacttccaga atacgtgagg aagcaaagcc gcccatcagc
     9241 gcctggcgca cacgtaccgg gtctgcaccg gcttttgaag caaacagcag agcttcagag
     9301 accgcttcaa tattgagcgc tacgataatc tgatttgcca ctttgcaggt ttgaccgtcg
     9361 ccgttaccgc ccacaagggt gatattttta ccgagcagtt caaacagcgg tttaacacgt
     9421 tcaaataccg cttcgtcacc gccaaccata attgacaacg tcccttcacg cgcgccgatt
     9481 tcgccgccgg agactggcgc atcgagataa tcaccaccca gttcattcac ctgacgagcg
     9541 aaacgtttag tttcaatcgg tgaaatggag ctcatatcaa caatggtttt gcccttcagc
     9601 gaggctttgg tacaaccatt ttcaccaaac agaacttctt caacctgagg tgtgtccggc
     9661 accataataa aaatgatgtc cgctgcttcc gttacctggc gcgcggtgtc gacattaaca
     9721 gcaccgagtg acagtaattc atcagcaacc ggtccaatgg tcgtgacatg taattgatga
     9781 ccggcacgcg ccagattaat ggccatcgga gtacccataa tgcctaagcc aataaatccc
     9841 agtttcataa ttaacctctt tttaaatgtc gctattcctg aattcagaca acacgatccc
     9901 ctgccaactt agcagcctaa aaaagcattg tctgaatagc gttacgtttt aacggtacgg
     9961 atccatccag cgtaaaccgg cttccgtggt ggtttggggt ttatattcac acccaaccca
    10021 accgttgtag tcagaatttt cgattacctt aaagagataa tcataattaa tttctccggt
    10081 ccccggttcg ccgcgatgcg gattatcggc aatttgcagg tgaccaattt
//
LOCUS       DAZKIP010000140        10066 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZKIP010000140 DAZKIP010000000
VERSION     DAZKIP010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10066
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.141"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            6..81
                     /locus_tag="ACHOUK_003987"
     tRNA            6..81
                     /locus_tag="ACHOUK_003987"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="ACHOUK_003988"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="ACHOUK_003988"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(467..1225)
                     /gene="yhdZ"
                     /locus_tag="ACHOUK_003989"
     CDS             complement(467..1225)
                     /gene="yhdZ"
                     /locus_tag="ACHOUK_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFP9899335.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1233..2336)
                     /gene="yhdY"
                     /locus_tag="ACHOUK_003990"
     CDS             complement(1233..2336)
                     /gene="yhdY"
                     /locus_tag="ACHOUK_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP9899336.1"
                     /translation="MTKVLLSHPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFALERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2346..3527)
                     /gene="yhdX"
                     /locus_tag="ACHOUK_003991"
     CDS             complement(2346..3527)
                     /gene="yhdX"
                     /locus_tag="ACHOUK_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP9899337.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3595..4620)
                     /locus_tag="ACHOUK_003992"
     CDS             complement(3595..4620)
                     /locus_tag="ACHOUK_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9899338.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(5051..5272)
                     /locus_tag="ACHOUK_003993"
     CDS             complement(5051..5272)
                     /locus_tag="ACHOUK_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899339.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            complement(5525..8674)
                     /gene="acrF"
                     /locus_tag="ACHOUK_003994"
                     /pseudo
     CDS             complement(5525..8674)
                     /gene="acrF"
                     /locus_tag="ACHOUK_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            complement(8686..9843)
                     /gene="acrE"
                     /locus_tag="ACHOUK_003995"
     CDS             complement(8686..9843)
                     /gene="acrE"
                     /locus_tag="ACHOUK_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFP9899340.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEVR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
BASE COUNT         2679 a         2593 c         2431 g         2363 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggactccc catgcgagag tagggaactg ccagacatca
      241 aataaaacaa aaggctcagt cggaagactg ggccttttgt tttatctgtt gtttgtcggt
      301 gaacactctc ccaaccagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg
      361 gcccggaggg tggcgggcag gacgcccgcc ataaactgcc agacatcaaa tcaagcgaaa
      421 ggccatccga aaggatggcc tttatgcttt tcgaactaac attcaattaa tggattacct
      481 gcgataaaaa tgccctcgta cgctctgact taggatgcgc aaaaaactca tccggtgcag
      541 cttgctccac tatttcgcca cgatccataa aaatcacccg gtcagcgacg gttcgcgcaa
      601 accccatctc atgggttaca cacaacattg tcatacccga ttgcgccagc ccaatcatcg
      661 tatccagcac ctctttcacc atctccggat cgagcgccga tgtgggctca tcaaacaaca
      721 taattttcgg cttcatacac agcgagcgcg caatggcaac gcgttgctgc tgaccacctg
      781 aaatctgtcc gggaaactta tgcgcatgtt cggcaattct cacccgctca aggtaatgca
      841 tcgccagagc ttcagcctct ttctttggca tcttgcgtac ccaaatcggt gccagggtac
      901 agttctgtag aacggtcaga tgagggaaga gattgaaatg ctgaaagacc attcccactt
      961 cctgcctgac gcgctcaata ttgcggatat cttcattaag ttcgatgcca tctaccacga
     1021 tccgtccctg ttgatgttct tccagatgat taatacaacg aatggttgtc gatttaccgg
     1081 aacctgaagg gccacacaga acgatccgtt ctcccggttg cacggttaaa tttatatttt
     1141 tcaaaacatg gaattgtcca taccatttat tgacgttttc cagcgtaatc atcgcgttag
     1201 caggttgcag taaaatttgg ctcatagtgt cctcaatgcg gtgtacgccc ggtgttaaaa
     1261 cgtttttcca gatgctggct atagcgcgac atgctgaaac agaagatcca gtagatcagc
     1321 gcggcgaaaa catacccttc cgtcgacata cccaaccagg cgggatcaac ggttgcttgc
     1381 tgaacgctac tgaaaagatc gaacaacccg ataatgatca ccaggctggt atctttgaag
     1441 agtgcgatga tggtatttac cagcccagga attaccagct tcaacgcctg tggcagaata
     1501 accagcccct gagttttcca gtaacccaac gccagcgact ctgccgcttc atattgccct
     1561 ttaggcagcg cctgtaatcc acctcgcacg acttccgcaa catatgctga ctgaaacagg
     1621 atcacgccaa ccagcgcgcg gatcagtttg tcgatactgg tgccttctgc cataaacaaa
     1681 ggcagcatga ccgaagacat aaacagaacg gtaatcagcg gcacgccgcg ccagaattcg
     1741 ataaaaatga ccgataagat gcgcacaatc ggcatatggg agcggcgacc taacgccagt
     1801 aagatccccc acggtagcgc ccccgcaatc ccaactgatg caataattaa agttagcgtc
     1861 agcccgcccc attgccgggt ttcaacccgc tcaagagcaa aaaaaccgcc atacatcagc
     1921 caccagacaa tcagtgggta aatcactgcc cagaccgcaa tatagcgacc gcgatgcggg
     1981 agtattttcc agaacattgg tgcgattgac acaaggccaa tcagtaatgc caagttaatt
     2041 cgccagcgtt ggtcgtgtgg gtaaagtcca tacataaact gaccaaatcg ttcgtggatg
     2101 aagacccaac aggcaccggc ttttgtgcag tcggcacgcg ttgagccaac ccagttagcc
     2161 tgcaaaaatg cccagttcag caacggtggg atcagttccc acatcaacca tatgcagcca
     2221 atagtcagca ggctattgct ccagctggag aacagatttt ttcgcaccca caccatcgcg
     2281 cggcttgagt tatggctcgc cgggcgcgag ggatgagaca gcaatacttt tgtcatcatg
     2341 actccttagc gttccacgat cgcgatgcgg cggttataga tattcatcag cagcgagata
     2401 gtcaggctga taatcagata gaccgacatg gtcatggcta tcgtctcgat ggcttgcccc
     2461 gtctggttca gcacggtgcc agcaaacagc gaaaccatat cgggatagcc aatagcggcg
     2521 gcaagagagg agtttttgac gatgttgaga tactggctgg ttaacggtgg aataatcacc
     2581 cgcagtgcct ggggaataat gacctggcgt agcgtaaccg ggttgggtaa tcccagtgac
     2641 cgagccgctt catgttgacc ataaggcact gcctggatcc ccgcgcggat aatctcggcg
     2701 ataaatgcag aggtataaac cgaaagtgcc agcgttaagg ctgccagttc aggaattaaa
     2761 accatcccgc cgcggaagtt aaagccttgt agggctggga catcccagtg tagtgctgcg
     2821 ccaaaaagcc attgcgccag taaaggcaaa ccgatgatca acactgcggc gatgggccag
     2881 gtgcggcgca gctgtccggt ctttatctgg tatgttttat taaagcggaa tagcccaaca
     2941 gaaaggacta tagccataac aacagccagg ataaacgcaa taaatccgtc tcccagctgc
     3001 ggtgacggaa tataaagccc acgattgctc aaaaaggcca gatcaaacgc gctcacagcc
     3061 tggcgtggtc cgggcaaatt gcgcaacacg gcaaagtacc agaagaagat ttgcagcagc
     3121 gggggaatat tacggaagat ctcaatataa attgtggaaa gctttcgtag cagccagtta
     3181 tccgaaagtc tcgccagacc gataaagaag cccaaaacag aagcgaacac aatacacaat
     3241 gcagaaacca gtagcgtatt gagtaagccg acaataaaaa cgcgtccgta ggtgtcgccc
     3301 tgctggtaat cgatcaaatg ctggacaata ccgaagccag cgccgcgatc cagaaaggca
     3361 aaacctgaag taatgccacg attattgaga ttcgttacag tgttgtgaaa taaccaacca
     3421 acaatgccga caacagcaac aacggcaagg atctggaata accaggcgcg aaccgtaggg
     3481 ttggcaaaag agagtgagcc tttaacggtt gagcggcgat gagacataga aacctcggaa
     3541 agcataacgc tgaagagagc gccgccgtag cgacgctcgt tacctcacac cgacttaacg
     3601 cacgggcggt gcgtactgaa taccgccgtt attccagaga ttattttgcc cacgtttaat
     3661 tttcagcggg ctttctgaac ctacgttacg ctcgaaaatt tccgagtagt tacccacctg
     3721 tttgatgatg ttataggccc atttattatc caacttcaga tccttgccgt aatcgccttc
     3781 tttacccagc agatgtgcca tatcaggcgt tgctggatta gccgcttttt catcgacgtt
     3841 ctgggaattg atgcccatct cttcagcatt tagcatggcg aaaagcgtcc agcgtacaat
     3901 cgagaaccat tcatcatcgc cacgacgaac taccggccca agcggttctt tagagataac
     3961 ctccggtaag acaatccatt cagccgggtt gcttaatttg atgcgcaggg catacagttg
     4021 ggattgatcc gaagccagcg tatcgcagcg accggattcc agtgccttcg ctgattcgtc
     4081 agaacgatcg aaagtcactg gtgtgtactt catattgttt gccttgaaat agtcggcgac
     4141 gttgagttcg gtatcagtac ccgcctgaat acagacggta gcgccatcca gttctttcgc
     4201 gctttttagc cccgctttat cgtgcgtcag gaagccaatg ccgtcgtaat aagtgacgcc
     4261 agtaaatgcc attcccatcc cggcatcgcg agatgaagtc caggtcgtat tacgggagag
     4321 caaatccact tcccctgact gtaaagccgt gaagcgttct tttgctgtga gtggggtata
     4381 tttcactttc gtgtcgtcac caaatacagc agcggcaaca ccacgacaaa catcaacatc
     4441 aatacctgaa aacttaccgt ctgcatcggc ataagagaac ccaggtaatc catcactgat
     4501 cccgcattgc acaaaacctt ttttctgaac ggcatcaagc gtcgcgccag catgcgcctg
     4561 atttgcaacg gcaagcagca cgctggcggc agccagtgtg gctatcatca tctttttcat
     4621 aatgcttcct gtgaggcgaa aattatcgtt ataggacgtt tgggaaacgt cttttgcctg
     4681 tctgtggcca tcctcttata agcaaaagta gtgccagctt ttgcgcacga tttcgcgtcg
     4741 caataagctg agtgtagaca ggaaataata aaaacagcgc cccgcaatgg tgcgaaaatg
     4801 aaacatgcgc catattttgg cgcaaaacat ttacgcgtgt tttactaagc agaataatga
     4861 ttgttagcgt gagtatattt ctggtgggaa gtgcgaaagt taaattgatg cgttatttcg
     4921 aaagcagcga aataacacga tataacatgc taaatcagca gagaattagc cagcagaata
     4981 tagcaatatt ttatcttatg taaagatact gtatcgttaa acagagcgcg aaactccgcg
     5041 ccctgttcag ttatttcgac agagcgatga tccccatcgt caggccagca acagccgctg
     5101 ccccgccagc aatagcccca gccgtttccc agttatcctg agtcgtgcct ttcatacagg
     5161 tattggtgtg aaccagacga ccctgatcgt cataaaccgg aacgcagggc gaatcgtgag
     5221 cacagcccgc cagcaatgcg gtgagcaatg ccacaggaat taatcttttc atggtgttac
     5281 ctcagtaata aacgctaacg cttaataagt taactctctt attaacgagc tgaatgccat
     5341 tttatcaccg gaggttataa atcttgcgga atgaataatg acaaattatg tgaattgtaa
     5401 aaatagtgaa gggagcggga gctatagaaa gcttttaaag agtgagcaaa agaatatctt
     5461 attaattttt atgggtttca ctggaaaaca taaaggcacc cgaaagcgcc tttatgtttc
     5521 agatttatcc tttaaagcaa cggcggatca ccacaaagaa caccggtacg aagaagattg
     5581 ccagcaacgt tgcagagacc attcctccca ttaccccgat acccactgcg ttctgcgcgc
     5641 cactgccggc accgttactg atagccagcg gtaatacgcc gagaataaag gcgagagagg
     5701 tcatcaggat aggacgcaga cgcatacgta ctgccattag tgtcgcttca acaacacctt
     5761 taccctcttt ctccatgaga tctttagcga actcaacaat caaaatagcg tttttggccg
     5821 acaagccaat tgtcgttagc aagcctacca taaagtagac gtcatttttt tgattaaaga
     5881 gtgtcgccgc cagcagcacg ccgacaatcc ctaacggcac gaccaacata accgagacag
     5941 gaattgacca gctttcatag agtgcagcaa ggcacaggaa aacaaccaca aaggaaattg
     6001 ctaccagagc gggagcctgg tttcccgata agcgttcctg atacgacata cccgtccagt
     6061 cataaccaat gcccgcaggt aattttgacg ctaggttttc catcaacgcc atggcatcgc
     6121 cggaactggt tcctggcgcg gcttccccct gaatctccat tgacggcaga ccgttgtagc
     6181 gttccagtcg cggagagcca tacacccaat gtgaagtggt aaaggcagag aatggcacca
     6241 tttcgccgtt ggtgctgcgg acataaagtt tatcgacatc ttctggcagc atacggaatt
     6301 tggcatccgc ctgaacatac acctttttca cgcgaccacg gtcgatgaag tcgttaacgt
     6361 aagtcccacc caacgccgtt gaaatggtct gattgatgtc agaaagtgaa acacctaatg
     6421 cctgcgcctt ttcctggtca acttccagtt taaactgcgc ggtgtcttcc aggccattgg
     6481 gacgcacgct gactaagctg gcaggatgtt gcgccgccat accaagcaac tggttacggg
     6541 cctgggttag ggcatcgtga cccagcccag ccttatcaat taactcaaag tcgaaacccg
     6601 ttgccgtgcc cagttcaaca atggctggca tattgaatgg aatgacaaaa ccgtcgcgga
     6661 tcttgcccaa ctccattttg gcacgatgga ttaccgcttc cgcactgttt tcatcaccat
     6721 tacgctcttc ccacggtttc agactgacga aggccatacc tgtagattca attggtcaac
     6781 gcaacagtta tgtgaaaaca tggggttgcg gaggtgcctg gccgctgaag ctaaagccgt
     6841 taaccgtaaa gacactttca acgtttgctt tctcgttctt cagatagtaa tccgtcactt
     6901 gatccaacac tttttgcgtc cgctcttgcg tcgcgccagc gggtaactga atcatggtca
     6961 gaaagacacc ctgatcctct tcaggtaaga aggaagacgg aagacgtaaa aacaacacca
     7021 ccattcctgc aacaatcagc gcatagatca gtaaatatcg tcctgtggaa ccgaggattt
     7081 tgccgacgct gttggtgtag tggttaacgc tatgatcgaa ggtggtatta aaccagccga
     7141 agaaaccacc cttattttcg tgatgctcag ctgaggtggg tttgagcagt gttgcacata
     7201 acgcaggggt aagaatcaat gccaccagaa cagaaagcgc cattgccgaa acgatggtga
     7261 tagagaactg acgataaatt gccccagtag aaccgccgaa gaatgccatc ggaataaata
     7321 ccgctgacag caccatcgcg atacccacca gtgcgccctg tatttgcgac attgattttt
     7381 ccgtcgcttc ttttggcggg agcttatcct ccatcatcac gcgctcgacg ttctccacca
     7441 ccactatcgc atcatcgacg agcagcccga tggcaagcac catcccgaac atcgttagtg
     7501 tgttgatgga gtaaccaaaa gcggcgagga tggcaaacgt ccctaacaac accacgggta
     7561 ccgcaatggt ggggatcagc gttgctcgca tattctgcaa gaacagatac atcaccagga
     7621 acaccagcat aatagcttcg aacagtgttt ttaccacttc gtgaatagaa agctggacga
     7681 atggcgtggt gtcataaggg tagagaacct tcattccctg cgggaagaat ggctgtaatt
     7741 ccgccagttt tgccttaatg gctttcgcgg tatcgagagc attcgcgccg gttgccagct
     7801 taatacccag gcccgccgcc ggttttccgt tgatgcgagc gataacgtta tagttttcac
     7861 cgccaagttc aacccgtgcg acatctttca gacgcaccac cgagccgtca ctgtttacgc
     7921 gcagggtcac tttgccgaat tcttccggat tttttaaccg cgtctgagca ataatcgagg
     7981 cattcaactg ttgccctggt aacgctggcg ttccgcccaa ctgtccggca gcgatctgag
     8041 cgttctgtac cttcaactgg ttaatcacat caaccggtgt cagtttatat ttgtttagca
     8101 gatcggcgtc cagccagata cgcatcgcat actgtgcgcc gaaaagctgt acgtcaccga
     8161 cgctattcag acggctaagc gtatctttaa cgttagaggc cacatagtcc gagatatcgt
     8221 cctgtgtggt gtctgggtta tcagagacaa agcctgccac catcaaatag ctgctactgg
     8281 acttttcaac actgatcccc tgctgctgaa cctcctgcgg cagcaacggc gtggcgagct
     8341 ggagtttgtt ctgcacctgc acttgcgcga tatcaggatc ggtcccggac tggaaggtaa
     8401 gggtaattgt cacgctaccg gcggaatcgc tggtggagga catatacatc aggttatcga
     8461 taccgttcat attctgttcg ataacctgcg tcaccgtatc ctgcacggtc tgcgcatcag
     8521 cgcccggata gtttgctgaa acagaaaccg caggcggtgc aatggttgga tactgagcga
     8581 cgggcaattg taggatcgcc agtgcgcccg ccatcatcag aataatggcc agcacccatg
     8641 caaatatcgg tcgtcgaata aaaaagtttg ccatgtcaga ttaccttact tcgatgcagt
     8701 atctgcgggg gtatcggtag tggctttaac ctgctctccc ggacgcgctt tttgcaggcc
     8761 gctgacaatg acttgatcgc cagatttcag accttcgcta atcaaccatt tatcgccaat
     8821 cgcctgactg gcaacgacag ggcgcacttc cacctgactt ttatcgttaa caatcagcac
     8881 ggttgcatca ccacgcggtg tgcggctaac gccttgttgc gggatgagaa tggcgtcagg
     8941 ttggacgcct tcatcaatcc gtgcacgcac aaacataccc ggcaaaagcg tatgttgcgg
     9001 gttagggaag acagcacgta gggttatgga gccggtgctt tcatcaacgg tcacatcgga
     9061 gaattgcagc gtacctttca ggggataggt ttgaccgttt tccatgacca actctacgtt
     9121 gctggtggcg ttttccttat gcaaatttcc ttgctctacg gactgcttca gcctcataaa
     9181 atcgttgctg gattgggtca catcaacata gataggatcg agctgctgga cagttgccag
     9241 ttcagtcgtt tgcccattag tgacaagagc gccttcggtc acagtcgatt tgccgatacg
     9301 tccgctaatt ggcgcagtca ctttggtata agcaagattg atgcgagcgc tttcgactgt
     9361 ggcttttgcg gcaatcacgg cggcatcggc ctggcgagca tcagcaatgg cctggtcgta
     9421 ctcctgctgg ctgatgtatt tcgtacccac gagcggaacg taacgtttta ccgtcaaatg
     9481 cgcgatggcg gcggcggctt cacttttcgc cagttcgcct ttcgcgctgt cataatttgc
     9541 ctgataggtc gcgggatcga tctggtacag ggactggcct gcttgcacat cgctgccttc
     9601 agtgaaattg cgattcagta cgatcccgct aacctgtggg cgaacttcgg ctatacgata
     9661 agcattggtg cggcctggta attcagtctt aacttctaac ggggccgttt ttacaatatg
     9721 aacggtaacc tgcggttcac cgacgtgagc tttctcttct cccttatcgt tacaaccggc
     9781 gattaaagcc gcggagatca gaataaagga gggcaggagg aaaaacctgg catgtttcgt
     9841 cattactatt cctcaaaaaa ccaaaagcgc gttatttacc caaaaaggca acgcgttaac
     9901 tcgcagaaag aaaaatacag ttcgctatcc tacaaattat cattcgtcga tgtaaggaat
     9961 agttatgaat acaggcgtct caaggcacat aaacacaaaa aaagattaat attctactgt
    10021 tttattttga cgcgggttga aagaggcaga attaaaaaat cgtaaa
//
LOCUS       DAZKIP010000141         9894 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZKIP010000141 DAZKIP010000000
VERSION     DAZKIP010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.142"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            1..710
                     /gene="yneK"
                     /locus_tag="ACHOUK_003996"
                     /pseudo
     CDS             1..710
                     /gene="yneK"
                     /locus_tag="ACHOUK_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="putative protein YneK"
     gene            861..2051
                     /gene="ydeA"
                     /locus_tag="ACHOUK_003997"
     CDS             861..2051
                     /gene="ydeA"
                     /locus_tag="ACHOUK_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFP9899341.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGTVPAFAALIWSIIIFRRWPVTLE
                     EQMQ"
     gene            complement(2076..2741)
                     /gene="marC"
                     /locus_tag="ACHOUK_003998"
     CDS             complement(2076..2741)
                     /gene="marC"
                     /locus_tag="ACHOUK_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFP9899342.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNGAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TNH"
     gene            2953..3387
                     /gene="marR"
                     /locus_tag="ACHOUK_003999"
     CDS             2953..3387
                     /gene="marR"
                     /locus_tag="ACHOUK_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFP9899343.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            3407..3790
                     /gene="marA"
                     /locus_tag="ACHOUK_004000"
     CDS             3407..3790
                     /gene="marA"
                     /locus_tag="ACHOUK_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFP9899344.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            3822..4040
                     /gene="marB"
                     /locus_tag="ACHOUK_004001"
     CDS             3822..4040
                     /gene="marB"
                     /locus_tag="ACHOUK_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFP9899345.1"
                     /translation="MKPLLSAIAAALILFSAQGVAEQTQQPLVTSCGDVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQQAM"
     gene            complement(4071..4970)
                     /gene="eamA"
                     /locus_tag="ACHOUK_004002"
     CDS             complement(4071..4970)
                     /gene="eamA"
                     /locus_tag="ACHOUK_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFP9899346.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAISGVLVLIEDSFNGQHVAMLGFMLTLAAAFSWASGNI
                     FNKKIMSHSSRPAVMSLVVWSALIPIIPFFVASLILDGSGKMIHSLVTIDMTTILALM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMAGLYINVFGLQWRKAVKVRG"
     gene            5165..6352
                     /gene="ydeE"
                     /locus_tag="ACHOUK_004003"
     CDS             5165..6352
                     /gene="ydeE"
                     /locus_tag="ACHOUK_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFP9899347.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTVGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMFFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLIGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAVNPLVSGVVLTTLPSSSLFIILALVIIAAWALMLKGINARPQRQP
                     ALC"
     gene            complement(6393..6788)
                     /locus_tag="ACHOUK_004004"
     CDS             complement(6393..6788)
                     /locus_tag="ACHOUK_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFP9899348.1"
                     /translation="MRYVTEFTTAKPEVAEAHFLARLSVETDCADVHDSLAYIGDDFV
                     LLHVVGREEDFARRHIPGALHLPHKQMTAERMAEWPDDTLFVVYCAGPHCNGADRAAL
                     KLARLGKPVKIMLGGITGWEDEGYAFASE"
     gene            6883..7854
                     /gene="ftrA"
                     /locus_tag="ACHOUK_004005"
     CDS             6883..7854
                     /gene="ftrA"
                     /locus_tag="ACHOUK_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FtrA"
                     /protein_id="HFP9899349.1"
                     /translation="MTTLQRPLVIALAYDGLCTFEFGVAVEIFGLPRPELGDNWYRFA
                     VAAVEPGALQATGGIHVVADGGLELLPNADMIIIPGWRGIDAKVPEMLCQSLREAHQR
                     GTRLVSICSGVFVLAAAGLLHQRKATTHWRYTEQLQHRYPSISVSDNVLYQDEGQVMT
                     SAGSAAGIDLCLHIVRKDFGHEIANNVARRLVIQPHRQGDQPQNLTRPMASPRESQTL
                     GALFDFLQQNLAQTHTVSSLAERVNMSPRTFLRRFEALTGMTPARWLLNERLIRVQDY
                     LQNSVFNIEKIAELTGFGNSALLRHHFHQRFLLSPSQYRKNSRSPSQ"
     gene            7962..8057
                     /gene="mgtS"
                     /locus_tag="ACHOUK_004006"
     CDS             7962..8057
                     /gene="mgtS"
                     /locus_tag="ACHOUK_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFP9899350.1"
                     /translation="MLGNMNVFMAILGIILFSGFLAAYFSHKWDD"
     gene            8057..8161
                     /gene="mgtT"
                     /locus_tag="ACHOUK_004007"
     CDS             8057..8161
                     /gene="mgtT"
                     /locus_tag="ACHOUK_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFP9899351.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(8276..9166)
                     /gene="dgcZ"
                     /locus_tag="ACHOUK_004008"
     CDS             complement(8276..9166)
                     /gene="dgcZ"
                     /locus_tag="ACHOUK_004008"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFP9899352.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLRPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGVLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEETLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(9421..9813)
                     /gene="ydeI"
                     /locus_tag="ACHOUK_004009"
     CDS             complement(9421..9813)
                     /gene="ydeI"
                     /locus_tag="ACHOUK_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFP9899353.1"
                     /translation="MKFQAIVLASFLVIPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKTMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPATVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT         2567 a         2460 c         2218 g         2649 t
ORIGIN      
        1 ctatggggcg cgtatttcag accggcgtcg agcgtgtgct ctttctgttt ctgaatgatt
       61 ttattgaaca attcccaatg atcaaccctg gcgttcccat caaaagagcg catacaccac
      121 atattgaacc cctgcctccg gatcaccata ccgctgcaga ttacttacgc cagtttgatt
      181 tgcttgtcct gaattttatc tctcgcggta attttgtcat actcccccga ttatggaata
      241 actctgaggt tcacagatgg ttcgtcaata aagatcctaa cttgatcacc gccattctcg
      301 acataacgga cagcgaatta aaagaggatt tattgcaaag cctgatggat tcattaggtt
      361 ctaacaaaca tgtactaccc taagtctgca tctgtttttt atccctttta gcagaacaag
      421 aatctcctca ttttcaggac ttgtttttat ttttcgccaa tatgttgtta cactatcacc
      481 aatttatgaa tcccaatgaa agtgatttga atgacgtgtt gatgccagca tcattaagtg
      541 atgataaaat tatcaaacat atggcgcgta agaccctcaa actgtttgta aaaaatgaaa
      601 caccgccaaa agttactcat gaagatctgg tgaaaaacag gcctcgctcc cctgtcagac
      661 cgcctatacc cgcaacctcc aaaacgccag acattcctga tcgtcattaa accgtgatgt
      721 ttaccatctc tctgacgcgt gaaagaatca gcgtcagaga aacggaaaac gcgatccaga
      781 tcacaaatgc attgtattca catcattaac cgttttaaga tcatttcatc actttttcgc
      841 aactcacccg ataatctgtt atgacaacaa acactgtttc ccgcaaagtg gcgtggctac
      901 gggtcgttac gctggcagtc gccgccttca tcttcaacac caccgaattt gtccctgttg
      961 gcctgctctc tgacattgcg caaagttttc acatgcaaac cgctcaggtc ggcatcatgt
     1021 tgaccattta cgcatgggta gtagcgctaa tgtcattgcc ttttatgtta atgaccagcc
     1081 aggttgaacg gcgcaaatta ctgatctgcc tgtttgtggt gtttattgcc agccacgtac
     1141 tgtcgttttt atcgtggagc tttaccgttc tggtgatcag tcgcattggt gtggcttttg
     1201 cacatgcgat tttctggtcg attacggcgt ccctggcgat ccgtatggct ccggccggga
     1261 agcgagcaca ggcattgagt ttaattgcca caggtacagc actggcaatg gtattaggat
     1321 tacctctcgg gcgcattgtg gggcagtatt tcggttggcg aatgaccttc ttcgcgattg
     1381 gtatcggggc gcttattacc cttttgtgcc tgattaagtt acttccctta ctgcccagtg
     1441 agcattccgg ttcattgaaa agcctcccgc tattattccg ccgcccggca ttgatgagca
     1501 tttatttgtt aactgtggtg gttgtcaccg cccattacac ggcatacagc tatattgagc
     1561 cttttgtgca aaacattgcg ggattcagcg ccaactttgc cacggcatta ctgttattac
     1621 tcggtggtgc gggcattatt ggcagcgtga ttttcggtaa actgggtaat caatatgcgt
     1681 ctgcgttggt aagtacggcg attgcgctgt tgctggtgtg cctggcactg ctgctacctg
     1741 cggcgaacag tgaaatacac ctcggggtgc tgagtatttt ctgggggatc gcgatgatga
     1801 tcatcgggct tggtatgcag gttaaagtgc tggcgctggc accagatgcc accgacgtcg
     1861 cgatggcgct attctccggg atatttaata ttggaatcgg ggcgggggcg ttggtaggta
     1921 atcaggtgag tctgcactgg tcaatgtcga tgattggtta tgtgggcacg gtgcctgctt
     1981 ttgccgcgtt aatatggtca atcattatat ttcgccgctg gccagtgaca ctcgaagaac
     2041 agatgcaata gttgaaaggc ccattcgggc cttttttaat gattcgtttt aatgatttcc
     2101 aggatgccgt taataataaa ctgtacaccc atacatacca gcaggaaccc catcaagcgg
     2161 gagatcgctt caatgccacc cttgcccacc agccgcataa ttgcgccaga gctgcgtaga
     2221 cttccccaca aaataaccgc caccaggaaa aagatcagcg gcggcgcaac catcagtacc
     2281 caatcagcga aggttgaact ctgacgcact gtggacgccg agctaataat catcgcaatg
     2341 gttcccggac cggcggtact tggcattgcc agtggtacaa aagcaatatt ggcgctgggt
     2401 tcatcttcca gctcttccga cttgcttttc gcctccggtg aatcaatcgc tttctgttgc
     2461 ggaaaaagca tccgaaaacc gataaacgcg acgattaagc cgcctgcaat tcgcagaccg
     2521 ggaatcgaaa tgccaaatgt atccatcacc agttgcccgg cgtaatacgc caccatcatg
     2581 atggcaaaga cgtacaccga ggccatcaac gactgacgat tacgttcggc accgttcatg
     2641 ttgcctgcca ggccaagaaa taacgcgaca gttgttaatg ggttagctaa cggcagtaac
     2701 accaccagcc ccaggccaat tgctttaaac aaatctaaca ttggtggttg ttatcctgtg
     2761 tatctgggtt atcagcgaaa agtataaggg gtaaacaagg ataaagtgtc actctttagc
     2821 taaccttgca tcgcattgac caaaacttga accgatttag caaaacgtgg catcggtcaa
     2881 ttcattcatt tgacttatac ttgcctgggc aatattatcc cctgcaacta attacttgcc
     2941 agggcaacta atgtgaaaag taccagcgat ctgttcaatg aaattattcc attgggtcgc
     3001 ttaatccata tggttaatca gaagaaagat cgtctgctta acgagtatct gtctccgctg
     3061 gatattaccg cggcacagtt taaggtgctc tgctctatcc gctgcgcggc gtgtattact
     3121 ccggttgaac tgaaaaaagt gttgtcggtc gacctgggag cactgacccg tatgctggat
     3181 cgcctggtct gtaaaggctg ggtagaaagg ttgccgaacc cgaatgataa gcgcggcgta
     3241 ctggtaaaac ttaccaccag cggcgcggca atatgtgaac aatgccatca attagttggc
     3301 caggacctgc atcaagaatt aacaaaaaac ctgacggcgg acgaagtggc aacacttgag
     3361 catttgctta agaaagtcct gccgtaaaca aaaaagaggt atgacgatgt ccagacgcaa
     3421 tactgacgct attaccattc atagcatttt ggactggatc gaggacaacc tggaatcacc
     3481 actgtcactg gagaaagtgt cagagcgttc gggttactct aaatggcacc tgcaacggat
     3541 gtttaaaaaa gaaaccggtc attcattagg ccaatacatc cgcagccgta agatgacgga
     3601 aatcgcgcaa aagctgaagg aaagtaacga gccgatactc tatctggcag aacgatatgg
     3661 cttcgagtcg caacaaactc tgacccgaac cttcaaaaat tactttgatg ttccgccgca
     3721 taaataccgg atgaccaata tgcagggcga atcgcgcttt ttacatccat taaaccatta
     3781 caacagctag ttgaaaacgt gacaacgtca ctgaggcaat tatgaaaccg cttttatccg
     3841 caatagcagc tgcgcttatt ctcttttccg cgcagggcgt tgcggaacaa acccagcaac
     3901 cgctcgttac ttcctgtggc gatgtggtgg ttgttccccc atcgcaggaa caaccaccgt
     3961 tcgatttaaa tcacatgggt acaggcagtg acaaatcgga tgcgctgggc gtgccctatt
     4021 ataaccaaca agccatgtag tatattcagg ccccggcata tcggggcttg ttaacctctt
     4081 accttcaccg ctttacgcca ctgcaaacca aatacattga tatacagtcc ggccataatg
     4141 agcacagccc ctaagaattg cagacctgtt aagcgttcat ccagcagtaa cgctgcactt
     4201 gccagaccga ctaccggcac cagtaatgat aatggtgcga cacgccatgt ttcatagcgt
     4261 ccaagcagcg taccccaaat cccgtagcca actatcgtcg ctacaaatgc cagatacatc
     4321 aacgccagaa tggtggtcat gtcgatagtg accagactgt gaatcatttt tccggagcca
     4381 tcgagaatga gcgaggcgac aaaaaacgga attatcggga ttaatgcgct ccagacgacc
     4441 agagacatta ccgccggacg ggatgaatga gacatgatct ttttgttgaa gatattgcca
     4501 ctcgcccaac taaatgccgc tgccagagtc aacataaagc cgagcatcgc cacatgctga
     4561 ccgttaaagc tatcttcgat taacaccagt acgccagaaa tcgctaacgc aatccccgcc
     4621 agttgcttac cgtgcagccg ttcgccaaaa gtaaacgcgc caagcacgat agtaaaaaac
     4681 gcctgagcct gtaagaccag cgaggccagt ccagccggca taccgaagtt aatggcgcag
     4741 aagagaaagg caaactgtgc aaaactgatg gttaaaccat accccagcag taaattcagc
     4801 ggtacttttg gtcgtgccac gaaaaagatt gccgggaagg cgaccagcat aaagcgcaaa
     4861 ccggccagca tcagcggtgg catgttatga agccccactt tgatgaccac aaaattaagc
     4921 ccccatacaa cgacgactag tagcgccaac accccatctt ttcgcgacat tctaccgcct
     4981 ctgaatttca tcttttgtaa gcaatcaact tagctgaatt tacttttctt taacagttga
     5041 ttcgttagtc gccggttaca acggcattaa tgcgcaaata agtcgctata cttcggattt
     5101 ttgccatgct atttctttac atctctaaaa caaaacataa cgaaacgcac tgccggacag
     5161 acaaatgaac ttatccctac gacgctctac cagcgccctt cttgcctcgt cgttgttatt
     5221 aaccatcgga cgcggcgcca cgctgccatt tatgaccatt tacttgagtc gccagtacag
     5281 cctgagtgtc gatctaatcg gttatgcgat gacaattgcg ctcactattg gcgtcgtttt
     5341 tagcctcggt tttggtatcc tggcggataa gttcgacaag aaacgctata tgttactggc
     5401 aattaccgcc ttcgccagcg gttttattgc cattccttta gtgaataacg tgacgctggt
     5461 ggtgctcttt tttgccctca ttaactgcgc ctattccgtt tttgctaccg tgctgaaagc
     5521 ctggtttgcc gacaatcttt catccaccag caaaacgaag atcttctcga tcaactacac
     5581 catgctcaat attggctgga ccgtcggccc gccactcggc acgctgttgg tcatgcagag
     5641 catcaatctg cccttttggc tggccgctat ctgctctgcg tttcccatgt ttttcattca
     5701 aatttgggta aagcgcagcg agaaaatcat cgccacggaa acaggcagtg tctggtcgcc
     5761 gaaagtttta ttacaagata aggcactgtt gtggtttacc tgctctggct ttctggcttc
     5821 ttttgttagt ggcgcatttg cttcatgcat ttcacaatat gtgatggtga ttgctgatgg
     5881 tgattttgcc gaaaaggtgg tcgcggttgt tcttccagtg aatgctgcca tggtggttac
     5941 gttgcaatat tccgtgggcc gccgccttaa cccggctaac atccgcgcgc tgatgacagc
     6001 aggcaccctc tgtttcgtca tcggtctgat cggttttatt ttttccggca acagcctgct
     6061 attgtggggt atgtcagctg cggtatttac tgtcggtgaa ataatttatg cgccgggcga
     6121 gtatatgttg attgaccata ttgcgccgcc aggaatgaaa gccagctatt tttccgccca
     6181 gtcattaggc tggttgggcg cagcggtaaa tccattagtg agtggtgttg tacttaccac
     6241 tctcccgtcg tcttcgctgt ttatcattct ggcgttagtc atcattgccg cgtgggcgct
     6301 gatgttaaaa ggtattaacg ccagacccca gaggcaacca gcactctgtt gacgttcgtg
     6361 ccgttccact actggcacgg cttcgctaat cattattctg aggcaaaggc atagccttca
     6421 tcttcccagc cggttatccc gccgagcatg attttaaccg gctttccgag tcgagccagt
     6481 ttgagtgccg cacggtctgc accgttacaa tgtggccccg cgcaatacac cacaaatagc
     6541 gtatcgtcgg gccattcggc cattctttct gcggtcattt gcttatgggg taaatggaga
     6601 gcgcctggga tatgccgacg cgcgaagtcc tcttcacgtc caaccacatg aagtaaaacg
     6661 aaatcatcgc caatataagc cagagagtca tgaacatcgg cgcaatctgt ttcgacactc
     6721 aagcgcgcga gaaaatgcgc ctcagcaact tctggcttcg ctgtggtaaa ttcagtgaca
     6781 taacgcatat tgctttgtcc ttttggtttg ttgtgttaac aataatgtaa ctaatctacc
     6841 tttcactgac cgcctctcga tatgccagaa atgagcaaaa tgatgacaac gttacaacgt
     6901 ccgctggtca ttgcactggc atatgacggg ctatgtactt ttgaatttgg cgttgccgtt
     6961 gaaatattcg gcctgccacg accggaactg ggcgataact ggtatcgttt tgctgttgct
     7021 gcggtagaac caggtgcgct acaagccacc ggggggatcc acgtggttgc tgatggtgga
     7081 ctggagttat tgcccaacgc cgatatgatt attattcctg gctggcgcgg aatagatgcc
     7141 aaagtgccgg agatgctatg ccagtcattg cgcgaggcac accagcgcgg tactcgcctg
     7201 gtatcaatat gctctggcgt gtttgtcctt gctgctgcgg gccttcttca tcagcgaaaa
     7261 gcaaccaccc actggcgcta cactgaacaa ctccagcacc gttacccttc aatcagcgta
     7321 agtgacaatg tgttatatca ggatgaagga caagtgatga cctccgccgg aagcgccgcg
     7381 gggatcgatc tgtgtttaca tatcgtgcgc aaagattttg gtcatgagat tgctaataat
     7441 gtcgcccggc gactggtcat tcagccacat cgccaggggg atcaaccaca aaatctgacg
     7501 cgcccaatgg cctcaccgcg tgaaagccag acgttaggtg ccttattcga ctttttgcag
     7561 cagaatctgg cacaaacaca tactgtgtct tccctcgccg aacgggtaaa tatgagtccg
     7621 cgcacttttt taagacgttt tgaggcgtta actggcatga cgcccgcccg ctggctgctc
     7681 aatgagcgct taatacgagt acaggattat ctgcaaaatt cggtttttaa catcgagaaa
     7741 attgccgagc tgacgggttt tggcaattca gcgttattgc gtcatcactt tcaccaacgt
     7801 tttttattat ctccttcgca atatcgcaaa aacagccgtt ccccatcaca ataatcgcct
     7861 gattcagcct ttgtttagat aacctctgct aatatctttc tcattgagat aaaaattaag
     7921 gtaagcgagg aaacacacca caccataaac ggaggcaaat aatgctgggt aatatgaatg
     7981 tttttatggc catactggga ataattttat tttctggttt tctggccgcg tatttcagcc
     8041 acaaatggga tgactaatga acggagataa tccctcacct aaccggcccc ttgttacagt
     8101 tgtgtacaag gggcctgatt tttatgacgg cgaaaaaaaa ccgccagtaa accggcggtg
     8161 aatgcttgca tggatagatt tgtgttttgc ttttacgcta acaggcattt tcctgcactg
     8221 ataacggatc gttgacacag tagcatcagt tttctcaatg aatgttaaac ggagcttaaa
     8281 ctcggttaat cacattttgt tcgtcaataa acatgcagcg atttcttccg gtttgcttac
     8341 cctcatacat tgcccggtct gctcttccaa tgaccacatc cagagtctct tcaggaaatg
     8401 cgcgactcac acctgctgtc acggtaatgt tgatatgccc ttcagaatgt gtgatggcat
     8461 ggttatcgac taactggcaa attctgacac ctgcacgaca tgcttcttca tcattagccg
     8521 ctttgacaat aatgataaat tcttcgcccc cgtagcgata aaccgtttcg taatcacgcg
     8581 tccaactggc taagtaagtt gccagggtgc gtaatactac atcgccgatt aaatgcccgt
     8641 aggtatcatt aaccaattta aatcggtcaa tatccaacaa cattaaataa agattcagag
     8701 gctcagtgtt gcgtaactga tgatcaaagg attcatcaag aacccgacgc cccggcaatc
     8761 ccgtcaaaac atccatattg ctacggatcg tcagcaaata aattttgtaa tcggttaatg
     8821 ccgcagtaaa agaaagcacc ccctcctgaa aggcgtcgaa atgcgcgtcc tgccagtgat
     8881 tttcaacaat agccagcatt aattcccgac cacaattatg catatgttga tgggcagaat
     8941 ccattagccg aacgtaaggt aattcatcgt tatcgagtgg cctcagatga tcaatccacc
     9001 gaccaaactg gcacagtcca taagaatggt tatccgttat ttctggctta ctggcatctc
     9061 tcgcgaccac gctgtgaaac atactcacca gccactgata gtgggcatcg atagccttat
     9121 tgagatttaa caagatggca tcaatttccg ttgtcttctt gatcattgcc actccttttt
     9181 cacagttcct tgtgcgcgct attctaacga gagaaaagca aaattacgtc aatattttca
     9241 tagaaatccg aagttatgat tcatctctga gataacattg tgatttaaaa caaaatcagc
     9301 ggataaaaaa gtgtttaatt ctgtaaatta cctcttcatt atcgcaaatg aaaggatgac
     9361 aaatagcata acccagtacc caaatggccc ggtagttcag gccatcaggc taatttattt
     9421 ttatttctgc aaatgagtga cccgaacgac ggccggcgcg cttttcttat ccagactgcc
     9481 gctaatgttg atcatctggt ccggctgaac ttctcgtcca tcaaaaacgg tcgcaggaat
     9541 aacaacatta atttcaccac tcttatcgcg aaaaacgtaa cggtcctctc ctttgtgaga
     9601 aatcaaatta ccgcgtagtg aaaccgaagc gccatcgtgc atggtttttg cgaaatcaac
     9661 agtcattttt tttgcatcat cggttccgcg atagccatct tctattgcat gaggcggcgg
     9721 tggcgctgca tcctgtttta aaccgccctg gtcatctgcc aacgcataag gtatgacaag
     9781 aaaacttgct aatacaatgg cctgaaattt catactaact ccttaattgc gtttggtttg
     9841 acttattaag tctggttgct atttttataa ttgccaaata agaatactgc caat
//
LOCUS       DAZKIP010000142         9840 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZKIP010000142 DAZKIP010000000
VERSION     DAZKIP010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9840
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.143"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            347..961
                     /gene="evgA"
                     /locus_tag="ACHOUK_004010"
     CDS             347..961
                     /gene="evgA"
                     /locus_tag="ACHOUK_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFP9899354.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            966..4559
                     /gene="evgS"
                     /locus_tag="ACHOUK_004011"
     CDS             966..4559
                     /gene="evgS"
                     /locus_tag="ACHOUK_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFP9899355.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     ESPFKDVFSNTHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASEHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEIIQQVAAVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNVDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLNCGMNLCLFKPLTLDVLKTHLSQLHQVAHIVPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQQNN"
     gene            complement(4615..5760)
                     /gene="yfdE"
                     /locus_tag="ACHOUK_004012"
     CDS             complement(4615..5760)
                     /gene="yfdE"
                     /locus_tag="ACHOUK_004012"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFP9899356.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGLSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQTILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(5834..6778)
                     /gene="yfdV"
                     /locus_tag="ACHOUK_004013"
     CDS             complement(5834..6778)
                     /gene="yfdV"
                     /locus_tag="ACHOUK_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFP9899357.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(6848..8542)
                     /gene="oxc"
                     /locus_tag="ACHOUK_004014"
     CDS             complement(6848..8542)
                     /gene="oxc"
                     /locus_tag="ACHOUK_004014"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFP9899358.1"
                     /translation="MSDQLQMTYGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(8596..>9840)
                     /gene="frc"
                     /locus_tag="ACHOUK_004015"
     CDS             complement(8596..>9840)
                     /gene="frc"
                     /locus_tag="ACHOUK_004015"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFP9899359.1"
                     /translation="TPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDVTR
                     HQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAIDH
                     MGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPPLV
                     SAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRLDK
                     LGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQNWE
                     NTCKAIGKPDWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIPCA
                     PVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEHTA
                     AVLQELGYSDDEIAAMKQNHAI"
BASE COUNT         2907 a         2234 c         2035 g         2664 t
ORIGIN      
        1 acacagtgca tgtgttgcac attatccctg gaacgttgga ggcctaattt aaatgaggtg
       61 caaaaaaaac aacagaggac taaaccgtgg cttttgcaat acaattctta cgcctgtagg
      121 attagtgaga agacttatag tgctaacttg aaactataaa tcatcggtat aatccctgat
      181 tttattgttg acatttcata tatgccaact atttatatgg tatacttgtc gaattatctt
      241 aaaggaagct caaattttct tatttttatt gagaaaatga gatgatgcct tatgtctgta
      301 ttactacagg gagaagggag atgcttcatt gcaaagggaa taatctatga acgcaataat
      361 tattgatgac catcctcttg ctatcgcagc aattcgtaat ttattgatca aaaacgatat
      421 tgaaatctta gcagagttga ctgaaggcgg aagtgccgtt cagcgggtgg aaacacttaa
      481 gcctgatatc gtcatcattg atgtcgatat ccccggagtt aacggtatcc aggtgttaga
      541 aacgctgagg aagcgccaat atagcggaat tattattatc gtctccgcta aaaatgacca
      601 tttttacggg aaacattgtg ctgatgctgg cgctaatggt ttcgtgagta aaaaagaagg
      661 catgaacaat atcattgcgg ctattgaagc tgcaaaaaat ggctactgct atttcccctt
      721 ctctctcaac cggtttgttg gaagtttaac gtccgaccag caaaaactcg actccttatc
      781 gaaacaagaa attagtgtca tgcggtatat tcttgatggc aaggataata atgacattgc
      841 tgaaaaaatg ttcatcagca acaaaactgt cagcacttat aaaagtcgcc tgatggaaaa
      901 attagaatgt aaatcactga tggatcttta cacattcgca caacgtaaca aaatcggcta
      961 accacatgaa gtttttaccc tatatttttc ttctctgttg tggtctttgg tcgaccataa
     1021 gtttcgcaga cgaagattac atcgaatatc gtggcatcag tagtaacaac cgtgtcacac
     1081 ttgatccact acgtctgagc aacaaggaat tacgttggtt agcgagcaaa aaaaatcttg
     1141 tgattgcagt acacaagtcc caaacggcta cgttgttgca taccgattcg cagcaacggg
     1201 ttcgtggtat taatgctgat tatttaaatc ttttaaaaag agcgttaaat atcaaattaa
     1261 cactccggga atacgcagat catcaaaaag caatggacgc gctggaagaa ggtgaagtcg
     1321 atatagtgtt atcacattta gttgcttcgc cgcctcttaa tgatgacatt gctgcaacca
     1381 aaccactgat aattaccttt ccggcgctgg tcaccaccct tcacgattca atgcgaccgc
     1441 ttacctcatc aaaaccagta aatattgctc gagtagcaaa ttatccccct gacgaggtaa
     1501 ttcatcaatc atttccaaaa gcaacaatta tctcttttac aaatttatat caggcattag
     1561 catccgtctc agccggacag aatgattact ttattggtag taacatcatt accagcagta
     1621 tgatttcccg ctatttcact cactccttaa atgtagtgaa atattataac tcaccgcgtc
     1681 aatataactt cttcttaacc agaaaagaat ctgtcattct taatgaagta ctcaatcgat
     1741 ttgttgatgc tttaacaaat gaagttcgct atgaagtatc acaaaattgg cttgatacag
     1801 gaaacctggc ctttctgaac aagccattag aactcactga acatgaaaaa cagtggatta
     1861 agcagcatcc cgatttaaag gtgctggaaa atccttactc gcccccctat tctatgacgg
     1921 atgaaaatgg ctcggttcgg ggcgttatgg gggacattct taatattatt accttgcaaa
     1981 caggtttaaa tttttctccg atcaccgttt cacacaatat ccatgctgga acacagctga
     2041 atcccggcgg atgggatata ttacccggtg ctatttatag tgaagataga gaaaataatg
     2101 ttttatttgc tgaagccttc ataacaacgc cttacgtttt tgtcatgcaa aaagcgcctg
     2161 acagtgaaca aacattaaaa aaaggaatga aagttgccat tccatattat tatgagcttc
     2221 attcgcaatt aaaagagatg tatccggagg tagagtggat aaaagtcgat aacgccagcg
     2281 ctgcatttca caaggtcaag gaaggcgaac ttgatgctct ggtcgcgaca cagttaaatt
     2341 cacgttacat gatcgaccat tactatccta atgaactgta tcattttctt attcccggcg
     2401 ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga accggaactt aaggatatta
     2461 ttaataaagc actgaatgca atccccccaa gcgaagttct gcgcctgacc gaaaaatgga
     2521 ttaaaatgcc caatgtgacc attgacacat gggacctcta tagcgagcaa ttttatattg
     2581 ttacgacatt atccgtttta ttagttggca gtagcctttt atggggattc tacctgttac
     2641 gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga aaaccaaata tcattccgga
     2701 aagcgctctc ggactcctta ccgaatccaa cttatgttgt aaactggcaa ggtaatgtca
     2761 ttagtcacaa tagtgcattt gaacattatt tcactgctga ttactacaaa aatgcaatgt
     2821 tgccattaga aaacagtgaa tcacccttta aagatgtttt ttctaatacg catgaagtca
     2881 cagcagagac gaaagaaaac cgaacaatat acacacaggt atttgaaatt gataatggca
     2941 tcgagaaaag atgcattaat cactggcata cattatgtaa tctgccagcc agcgaacatg
     3001 ctgtttatat ttgtggttgg caagatatta ccgagacgcg tgatttaatt catgcactcg
     3061 aagtagagag aaataaagcg atcaatgcaa ctgtcgcaaa aagtcagttt ctggcaacaa
     3121 tgagtcacga aataagaaca ccaataagct ccattatggg cttcctggaa cttctgtcgg
     3181 gttctggtct tagcaaggag caacgggtgg aggcgatttc actagcctac gccaccggac
     3241 aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga caaaattgaa tcgggtaact
     3301 atcaacttca accacaatgg gtcgatatcc ctactttagt ccagaacact tgtcactctt
     3361 tcggtgcgat tgctgcaagc aaatcgatcg cattaagttg cagcagtaca tttcctgatc
     3421 attacctggt taagatcgac cctcaggcgt ttaagcaggt cttatcaaat ttactgagta
     3481 atgctctcaa atttaccacc gagggggcag taaaaattac gacctccctg gttcacattg
     3541 atgacaacca cgctgtaatc aaaatgacga ttatggattc tggaagtgga ttatcacagg
     3601 aagaacaaca acaactgttt aaacgttata gccaaacaag tgcaggtcgt cagcaaacag
     3661 gttctggttt aggcttaatg atctgcaaag aattaattaa aaacatgcag ggtgatttgt
     3721 cattagaaag tcatccaggc ataggaacaa catttacgat cacaatcccg gtagaaatta
     3781 tccaacaagt ggcggctgtc gaggcaaaag cagaacaacc catcacacta cctgaaaagt
     3841 tgagcatatt aatcgcggat gatcatccga ccaacaggct attactcaaa cgccagctaa
     3901 atctattagg atatgatgtt gatgaagcca ctgatggtgt gcaagcgcta cacaaagtca
     3961 gtatgcaaca ttatgatctg cttattactg acgttaatat gccgaatgtg gatggttttg
     4021 agttgactcg caaactccgt gagcaaaatt cgtccttacc catctggggg cttacagcca
     4081 acgcacaggc taacgaacgt gaaaaagggt taaattgcgg catgaactta tgtttgttca
     4141 aaccgttgac cctggatgta ctgaaaacac atttaagtca gttacaccag gttgcgcata
     4201 ttgtacctca gtatcgccac cttgatatcg aggccctgaa aaataatacg gcgaacgatc
     4261 tacaactgat gcaggagatt ctcatgactt tccagcatga aacacataaa gatttacccg
     4321 ctgcgtttca tgcactagaa gctggcgata atagaacttt ccatcagtgt attcatcgca
     4381 tccacggtgc ggctaacatc ctgaatttgc aaaagttgat taatattagc catcagttag
     4441 aaataacacc tgtttcagat gacagtaagc ctgaaattct tcagttgcta aactctgtaa
     4501 aagaacacat tgcagagcta gaccaggaaa ttgctgtttt ctgtcaacaa aataactaaa
     4561 tagtggctcc cacaatgttc aaatgtggga gctatttacc agcacatctt acatttatga
     4621 tgagaactcc tggcgaattt gctccccatg ctggtcgagc gttgccgctc ccggcataac
     4681 atgcgggtcc gcgcagccac tgatttttat cggattaccc ggcatcatta ttcccccggc
     4741 ttcaatcaac atatttctcg cctgagtttg tggcaaatta atggcctcag ccacacttaa
     4801 taacggcgcg acgggcacac caacttcatg tattctggct aaccaaactt cagctgcctg
     4861 tgtttttaac gtccgctcaa tatattgttt aagaatagtc tggttttgta cgcgtaaaat
     4921 attgctgcta aatctgggat cattaaccag ttccgtaagc tccagtgcct ggcataacgc
     4981 agagaaaagc ttgtcattac cacaacaaat cgtaatcggc ttatcctgag tatcgaaaac
     5041 atcaaaaggt gccatgtagg ggtggcgatt tcccaggcgt tgtggtgact tccctgtcgc
     5101 aatatatgcc atcagaccat gctccagaaa actcagcgtg gcatcaaaca tcgctatatc
     5161 gacatgcgcc cctctttggc tcttttcgcg gccataaagt gcactcacta ttccgctgaa
     5221 taaataaaca ccgccgcata gatccgcaag agaggtacca acacgcactg gcggagcatc
     5281 agggtaccct gtttccatca ttatcccgct cattgcctga atgatggtat cgtaggcagg
     5341 agcatctttt agcggaccgg tatgtccgaa acctgacgat gaagcatata tgaggcgcgg
     5401 gttgatttct tgtaaccttt cccatgaaag ccccagtttt tccattgtac ctgggcgaaa
     5461 attctcagct aatacatcag cttgtttaag catatttata aatatacttt tatcgtgatc
     5521 attctttaaa tcaagaacca cactctcttt accatgatta ataaaactgt aatagagtga
     5581 ctgtccatcc acatagggac caaatgtgcg ggtatcatca ccatgacccg gcggctcaac
     5641 tttaattacc cttgcgccca tattacaaag aagttgagtt ccgaaaggtc cattaaggac
     5701 atgtgtcata tcgataacta ataagccttc aaacggccct ttgctttcat tatttgtcat
     5761 ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact tcggcattta tgtaaaatca
     5821 ataacatgat taattatgaa accagtcgac tgacataaat ccacaaggga gccgtgacga
     5881 caaaacccag tacgctcaca gccaatgacg ctgtaccagt gcgggtgtag acattaaacc
     5941 ggctggcaat aatgattccg gagaatgccg gcggtaatgc gcctgccagt accatcatct
     6001 gcagatgttc gctgttcaaa tgacatgcca taccaacaag aagcagcgcc agtggcatca
     6061 gaatcagctt caggaaggtg ttataagcaa tttcggcact gaactcgaat ttatgtgcag
     6121 ccagagtcaa ccccgcagcg aataccgcta cccctgagtt agccttcgca atcagattaa
     6181 aggttgggtc ccatgctgcc ggaattttta ccccaaccaa caccaggatc gttgccagaa
     6241 caggtgccca tacaactggc tcctttgccg cagaaattaa tgcgctcaga ttactattct
     6301 tcttaccatc cgctcctgag gaaggattca gcaaatacag accaatagga atagtaattg
     6361 cgttaacaat aatagaaata attgctacca ctaaacctgt tgatacggaa tcaccataaa
     6421 taggatcgag aactgcaaac cccaggaatc caatggtagg tgaacctgca attaatgcac
     6481 atacagctgc ttctgcatgg gtacgtttaa aaaatttgta gcaaccaaac caggagaaaa
     6541 agaaacatcc gacaataacc acgagtgata caagggtcag acgagtgtcc gcaaaaatca
     6601 tttccctgtt tgcccgggta atagaaacaa atagggctgc aggaagcgcg tagtttaata
     6661 ccagtttatt aaatgcccgg gcttgatctt ctgaaaaagt ttctcgtctg ccgctaaaat
     6721 aacccaacag catgattacg ataataggca ataaatcgcc aataaaaaat gttagcataa
     6781 tagtatttcc gataagggtc ccctcaccat cgcataatga gttggcaaga ggagacgatg
     6841 tcagtattta attaccagcg acttgttttg ggttaagttt ggtaatatgg ccactttcag
     6901 ttcctgctgc agggtcgatg accacattaa taatggtcgg tttgcgcgac tgaataccgg
     6961 tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt atagccaacg ccacgaaacg
     7021 catccattaa tttgtcatac cttgcatggt gcaacagatc tgttggtgat ggtgcaccag
     7081 cgccactgag atcaacaccg tctcctctgt agatgccgcc attattaaaa ataacgatcg
     7141 ttaccggcag gttatatcga caaatcgttt caatttccat cccactgaaa ccaaaagcac
     7201 tatcaccttc aatggcgaca accggagaac cagaggtcac gctagcaccg atggcatagc
     7261 ccataccgat gcccatgaca ccccaggtac cacaatccag acgacgacgt ggtttataca
     7321 tatcaataat atttcgtgca ttatccaggg tatttgcacc ttcattaact aaataaatat
     7381 cctggttctc acgcaataca tcgcgcacag cacttaatgc attaaagtaa tttaatggtt
     7441 gggtatctgt acttaatttt tcatgcattt tttgtgcatt ttgctgcttg tggatattta
     7501 aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg tttcagctct gccagcatac
     7561 cttgcatact ggatgcaata tcgccaacga ctggcacagc aatggggcgg ttgctgtcaa
     7621 tttcctgcgg ttcaatatcc agttgaataa actgtgtatc tgccgcccat ccttttttac
     7681 cgtgtgccag taaccaattc agtcgtgcac caacaagcat gacaacgtca gcatttgcca
     7741 gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt atcttcgagg atccctttcg
     7801 ccatagacat tggcaggaat ggaatctggg cactttcaat aaattcacga agctgttcgt
     7861 cagcttgtga atacgccgcg cctttgccaa ggataattaa tggccgttca gcttttgcta
     7921 aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa taatgctggc gacggatttt
     7981 caacttttac aatcgtggtt aacgcttcgt ctttttccat cgtcgcggcc aggacatttg
     8041 ctggcaaatc aagataaact ccgccagggc gacccgatac tgagacccgg atagcgcgtg
     8101 ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa tgctgctttg gcatacggtt
     8161 ttgccgcatt catttggtcc agctcttcat aatcaccttg ctgtaggtcg acgatcgcgc
     8221 ggtcgctgga gccgctaatc atgatcatcg gaaaaccatt taccgttgcg ttggccaatg
     8281 cggtcaaacc attgaggaat cctggcgcag acactgtcag gcagatccct ggtttttggg
     8341 taagaaaacc gcttgccgca gcggcatagc ctgccgactg ctcatgacga aaaccaatat
     8401 aacgaatgcc ttccgcctgg gcatggcgcg ccatatccgt cacaggaata cctacaacac
     8461 cataaatagt gtcaatatta ttctgtttta atgcttcaac gatgatatgc ataccatatg
     8521 tcatttgaag ttgatctgac atgattaata acctcgaaat ttttataaat tgggagccag
     8581 aagcccccgt taatatcaga tggcgtggtt ttgcttcatt gcagcaattt catcgtcgct
     8641 ataacccagc tcctgcaata ccgcagcggt atgttcacct aatagaggcg cagctttaat
     8701 atccggcgta aaggcagaga atttcattgg acaaccaact gtcagatatt ttccacgcaa
     8761 tggttgttcc acttcgacaa cactgccgct ttggcgtaaa gagggatcaa gtgaaatttc
     8821 tttcatactt aaaaccggtg cacaaggaat atcaaactga gtcaaataag ccactgcttc
     8881 atgtttatca atagtgacag tgtatttttc gatttcagca aaaatatcga aaatatgtgg
     8941 ctgtcgggca tgggctgtac tgtatgccgg atcggtaatc caatctggtt tgccgatggc
     9001 tttacaggtg ttttcccagt tttgctcctg aatagtgaaa taaatatagg cgttaggatc
     9061 agtttcccag cctttacatt tcaggatcca gccaggctga ccgccaccac ccgcattacc
     9121 tccgcgggga actgcatcac caaatgtgcc attcggatac tgcgggtatt cttccagata
     9181 acccaattta tcgagacgct gctggtcgcg taatttcacg cggcaaaggt tcaatacggc
     9241 atcctgcatt gacatggtga ctcgttgccc acgccccgtt ttttcgcgat gcagcaaagc
     9301 agcaagtaaa ccgatcagta aatgcattcc ggtgttgctg tcacccaacg ctgcagcgct
     9361 taccagcggc ggaccgtccc aaaaacccgt agtggatgcc gcgccacccg ctgcctgagc
     9421 aacgttttca taggctttta cattcacata aggcgaacac tcatcaaacc ctttgatcga
     9481 accaaaaatc agacgtggat tgatttcttg aatatgctcc caggtgaagc ccatgtgatc
     9541 aatggcccct ggatgaaagt tctcgactaa gatatcagct tcgcggatca gcttttccat
     9601 tacctctttg ccttccgctg ttttggtatt taactcaata gaacgtttgt tactgttaag
     9661 catggtgaag taaagcgcat cgatatcagg aatatctcgc agctggtgac gcgttacgtc
     9721 accaacgccg gggcgttcaa ttttaatgac gtcagcgcca aaccaggcca gcatttgagt
     9781 acaagatggg ccagattgca caccggtgaa atcgagaact ttaattcctt gaagtggagt
//
LOCUS       DAZKIP010000143         9792 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZKIP010000143 DAZKIP010000000
VERSION     DAZKIP010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.144"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..144)
                     /locus_tag="ACHOUK_004016"
     CDS             complement(<1..144)
                     /locus_tag="ACHOUK_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899360.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWPCG
                     EFFS"
     gene            222..1316
                     /gene="ddlA"
                     /locus_tag="ACHOUK_004017"
     CDS             222..1316
                     /gene="ddlA"
                     /locus_tag="ACHOUK_004017"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFP9899361.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1340..1552)
                     /gene="yaiZ"
                     /locus_tag="ACHOUK_004018"
     CDS             complement(1340..1552)
                     /gene="yaiZ"
                     /locus_tag="ACHOUK_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFP9899362.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1812..2120
                     /gene="yaiY"
                     /locus_tag="ACHOUK_004019"
     CDS             1812..2120
                     /gene="yaiY"
                     /locus_tag="ACHOUK_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFP9899363.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2179..3273)
                     /gene="yaiW"
                     /locus_tag="ACHOUK_004020"
     CDS             complement(2179..3273)
                     /gene="yaiW"
                     /locus_tag="ACHOUK_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFP9899364.1"
                     /translation="MSRVNHLSSLSFLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSGIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAAKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3286..4506)
                     /gene="sbmA"
                     /locus_tag="ACHOUK_004021"
     CDS             complement(3286..4506)
                     /gene="sbmA"
                     /locus_tag="ACHOUK_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFP9899365.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFALFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4858..6015
                     /gene="ampH"
                     /locus_tag="ACHOUK_004022"
     CDS             4858..6015
                     /gene="ampH"
                     /locus_tag="ACHOUK_004022"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFP9899366.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6016..6639)
                     /gene="iprA"
                     /locus_tag="ACHOUK_004023"
     CDS             complement(6016..6639)
                     /gene="iprA"
                     /locus_tag="ACHOUK_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFP9899367.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6727..9669)
                     /gene="ehaB"
                     /locus_tag="ACHOUK_004024"
     CDS             complement(6727..9669)
                     /gene="ehaB"
                     /locus_tag="ACHOUK_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaB"
                     /protein_id="HFP9899368.1"
                     /translation="MHSWKKKLIVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLTIKNSTIHGMITSECMTTDCADDRATGYVYDRLTLSVDNSTIDDNYEHYTYNGTYN
                     NAADTHVVDVYDMGTAITLDQEVDLSITNNSHVAGITLTQGYEWEDIDDNTVSTGVNS
                     SEVFNNTITVKDSTVTSGSWTDEGTTGWFGHTGNASNYSNTLTADDVAIAAIANPYAD
                     NAMQTTVTLDNSTLMGDVVFSSNFDENFFPQGANSYRDADGDVDTNGWDGTDRMDVTL
                     NNGSKWVGAAMSVHMVDEDGDGSYDGYAVGTEATATLLDIAANSLWPSSTVGVDNINT
                     QYDENGHIVGNEVYQSGLFNVTLNGGSEWDTTKSSLIDTLSINSGSQVNVADSRLISD
                     TVSLTGGSNLNIGEDGHVATNTLTIDNSTVKMSDDVSAGWGLEDAALYANTITVTNDG
                     LLDINVDQFDANPFQADTLNLTSTTDTNGNIHAGVFDIHSSDYVMDTDLVNDRTNDTT
                     KSNYGYGLIAMNSDGHLTINGNGDNDNTASIEAGQNEVDNNGDHVAAATGNYKVRIDN
                     ATGAGSIADYNGNELIYVNDKNSNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTD
                     YANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINY
                     DQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFA
                     NNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPY
                     GSISGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYV
                     YDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSA
                     NVGVKYTW"
BASE COUNT         2513 a         2432 c         2329 g         2518 t
ORIGIN      
        1 agaaaaaaac tcaccgcatg gccatttaag ccaggaataa attagcttcc cgttcatcat
       61 ttttgtgatc ttgcgcacat ctttgagata acgcactttc ttcaccagta cgttgcacgc
      121 tttgtcagca gaagattgca acaatctttc agaaggccgc gaatcccccg ttttgtcgtg
      181 gtaatgtatg cactttgtgt tagacgggat aggtttttaa gatggaaaaa ctgcgggtag
      241 gaatcgtttt tggtggtaaa tcagcggaac atgaagtgtc tctgcaatcg gcaaaaaaca
      301 ttgtcgatgc cattgataaa agtcgcttcg acgttgtgct gctgggcatt gataaacaag
      361 ggcaatggca cgtcagcgat gccagcaatt atctgctaaa tgcagacgat cctgcccata
      421 ttgcgttgcg cccttcggcg accagcctcg cccaggtgcc aggtaaacat gagcatcagc
      481 ttatcgacgc gcaaaacggt cagccgttgc cgacggtgga tgtcattttc ccgattgtcc
      541 acggtacgct gggcgaagat ggttccttgc agggaatgct gcgggtcgcc aatttaccgt
      601 ttgtaggttc tgatgttctg gcttcagcag cctgtatgga taaagatgtc accaaacgtc
      661 tgctacgcga tgccgggctg aacattgcgc catttattac cctgacgcgc gctaatcgtc
      721 acaacatcag ttttgccgaa gtggagtcta aactggggtt accactgttt gtaaaaccgg
      781 ctaatcaggg ctcttctgtt ggtgtcagca aagtaaccag tgaagaacaa tacgcaattg
      841 ccgtcgatct ggcgttcgag ttcgatcata aagtgatcgt tgagcaaggg atcaaaggtc
      901 gtgagatcga atgcgcagtt ctgggcaacg acaatccgca agccagcacc tgtggcgaga
      961 tcgtactcac cagcgatttc tatgcctacg acaccaagta cattgacgaa gatggcgcga
     1021 aagtggtagt tccggcagcc attgcgccag aaatcaacga taagatccgg gcgattgccg
     1081 ttcaggctta tcagacgttg ggatgcgcag gcatggcgcg tgtagacgtg tttttaaccc
     1141 cagagaacga agtggtgatc aacgagatca acacactgcc tggcttcacc aacatcagta
     1201 tgtatccgaa gctgtggcaa gccagcggtc tgggttacac cgatctgatc acacgtttga
     1261 ttgagctggc gctggagcgt cacgctgcgg ataacgcatt gaaaaccaca atgtaatatt
     1321 ttaacgcccg ataatattct tattgggcgt tttcagtttc ttcatcgcga cgacgaataa
     1381 tcaaccccgc cagccagaaa ctaatcaccc acgtcaccag accgacggca taggtctgcc
     1441 aaccttttgc ttcaaatccc agtaacccca ccacgccatt aagaatgaat ataaggccaa
     1501 tggcgaacgc atagtagtgc cagtcacggc ggatttttac aggcagattc atggctgctc
     1561 cgttaacgaa aaacaacatc cttgcatatt ctgcaacaac cgtctgtggt gtagacaaca
     1621 ttttcgcaat tgttaataaa tttaacttaa agttgctatt ttgtgactcg caacgaagat
     1681 tcacgcttcg ggaatattcc caacatcaat ttaccatgat tctgatattg acaatctaaa
     1741 tgacctgtca gacttgccat aagccgctgg tatcacacca gaagagaagc agatgacttc
     1801 cggaggtttt tatggctgat ttcaccctgt caaaatcgct gtttagcgga aaatatcgca
     1861 atgcctcttc aacgcctggc aacattgcct atgcgttgtt tgtgctgttt tgcttttggg
     1921 ctggggcgca attgctgaac ctgttagtgc atgcgcccgg cgtctatgag cgtttaatgc
     1981 aggtccagga aacaggtcgc ccacgggtgg aaattggttt aggtgtcggc accattttcg
     2041 ggctgatccc gtttttagta ggctgcctca tttttgcagt ggtggcgcta tggctgcact
     2101 ggcgacatcg ccgccagtaa gatttacgtt gccagatacg gcctaaaacg ccttatctgg
     2161 catgacattc ctgtttgttc actgtttcat acaacgcgcc cgccgttcgt ctacgcgctt
     2221 cgcaaaccag gccgtagtca ggttgcgggt gattttcggg ctttccagtt gaatgccagg
     2281 caacatttcg cgggggagtg atttacccgt tttcgcttcg gcaagttgat aaactttctt
     2341 gtacagcgcc gtctcctcaa aagagaagct gtcacctttc tctagctgac gacgaatttc
     2401 gctgtcgctc atccccagtt ttgccgccag tttgcgcgtt gccagttcgg ttttcccggg
     2461 ttctttactg tcgtaacgaa ttaaatcacc gtccagtgcc agcttcacgc cgctggcctt
     2521 actgaccgcg ttctgaaacg cggcattacg gctggcgtac cagccagcgt taaaatcagc
     2581 aaaacggtat atcggtgcgc tataactggc gggataattc agtaaatggt aagtaccaaa
     2641 ccacaacccg ccgcggcgac taaagacttc ctgacggact gtaccgtcca ttttccacgg
     2701 ataccctttg gtatgctgtt cggcaaaagc aatgctgacc tgcatcggcc caccggtgcg
     2761 caccggatta agcgaaccaa acaacgtctg ccccattggc accatgctga tcagatcgtc
     2821 aaaaatcccg ctcaattgct tttctgtacg caccgaatct aaccgttcgc tataactttt
     2881 gccgtttggc gatttgattt ttagcgcagt atgaaccaga aaagcaggaa tgtgcatccg
     2941 ttcagcacga cggtcaatct cttgccaggc gatcttgctt aagcccggaa cggccggatc
     3001 tgcctgataa tttgactcct gctgcgccac cgccagcacc gagcagacgt tttccagcgt
     3061 cggtgccagc ccctgacttt caaaggtggt ggcgagatct ttcgcccagg catcgcggtc
     3121 tttcacgctc gcaggcatct tctgccgcac cacgctagcc acatctatcg ctttttcgcc
     3181 ctttttcaac ggctgtggcg cctgactgct acaccccgct aaaaccagaa ccgccaaaaa
     3241 tgaaagcgaa gacagatgat tgactcgcga cattacttct cctttttagc tcaaggtatg
     3301 ggttacttcc tgaattttgt caccatccag ctcatgttca aagctgcgca gacgtttgta
     3361 gatagacatc aactcaacca gtgtggtcca tgagttaatc aggtactgga aagcaccgcg
     3421 aacctgacca aaaacgttgg taatctgcgt catcaggccg agcgtaatcg taccggcaac
     3481 aattgacgga aacagcaaga acaaaccgaa aacgttatcg acctgcaaat agagaatgcg
     3541 agcgatgttg aaatacatat agtgaaaata gaggcggaaa tagtttttcc gtacggcgct
     3601 aaacagctcg cgtaccgttg gcggcgtcgc gcgcgtggca tcgtcttcac cataaaccag
     3661 ctctttacgg taggcagcct ctacacgctg gtttttaaac tccagccccg gcagtttgat
     3721 ccctaccact gccagcaatc cggtccccat cagcgaccag acgatagcgg caatcaccag
     3781 accatacgga atgtgcccga taatcggcag ctccggcaca tgcgcggaga gcgttaccaa
     3841 caccggcagg aaggcgatca acgtcatgat ggcgttgata aaactgacac ccatattctc
     3901 cagcgttgaa gcaaaacgca tggtgtcttc ctgcacacgc tgtgcggcgc cttcgatatg
     3961 acgcagttgt tgccagttcg ccatgtaata ttcgttcatc gccgtacgcc agcggaacac
     4021 gtagtgactg acaaagaagt tattcagcac actgatcact accgcgatca gcgcaatccc
     4081 cagaaagacg cccacttcgc ggtaaaattg ttcgatagtg actttatgcg gcgaacttag
     4141 cgcggtttga atcagatcat agaacggtgc ataccaggcg ttgacggcaa ccccaacttc
     4201 caccaaaaac caggtgacga agatgatcag tgcagtaccg agtattgacc agtattgcca
     4261 acggtgcggg ctgtagataa accagaacaa tgcaaaaagt cctacgcaaa caatgtagta
     4321 agcgtaaaaa atcaggaaat ccaacgacca gaaacgcgcg gcgctaatcg ggatctgccc
     4381 ggaagcgccg gtgatacgcg ccacccagtc ccccccaccg gcttgccaga agataacggc
     4441 aatcaatgcc caaacaaagg ccgagagaaa aaacgttccc ggctttggga aaaaagactt
     4501 aaacatatgc actcctgcta acttcttatc gttttaacaa ctgctgtgta ttaaccgcat
     4561 gaccgctaca aagcgtcagc caaatgaaac cgcccattgt gactcatagc aaggcgctta
     4621 gttcgccagc ctggccataa gaattgtttc gacggatttc acaaaccaaa gattgtttac
     4681 ataagcggat taacacgcgc aggatcacaa atgcaggcat tggtgagcag gaaaaagatc
     4741 gtgtttacgc gccgttacgt ttttaacact attattgcac taattcgccc tttgcaatcc
     4801 atcaatgagt agtataaata cgctcagtta ccttcattca atttatggac accaccgttg
     4861 aaacgtagtc tgcttttttc tgccgtgctg tgtgcggcgt cattgacctc cgtccatgcg
     4921 gcgcagccga tcactgaacc ggagtttgcc tctgatattg tcgatcgtta tgccgatcat
     4981 attttttacg gcagcggtgc cacggggatg gcgctggttg ttatcgacgg taaccagcgc
     5041 gtctttcgca gttatggcga aacgcgacct ggtaataacg ttcgcccgca gctggattcg
     5101 gtcgtgcgta ttgcttccct caccaagcta atgaccagtg agatgctggt gaaattgctc
     5161 gaccagggga ccgtgaagtt aaacgatccg ctaagtaaat atgctccgcc aggcgcacgc
     5221 gtgccaacct acaatgggac gcccattacg ctggtcaatc tggcaaccca taccagcgcc
     5281 ctgccccgtg aacagcccgg tggcgcggca catcgtccgg tgtttgtctg gccaacgcgc
     5341 gagcaacgct ggaaatacct ttctacggcg aagctgaaag ccgcgccagg cagtcaggcg
     5401 gcgtactcta accttgcgtt cgacctgctg gcagatgcac tggcgaatgc gtccggcaag
     5461 ccttataccc agttgtttga agagcagatt acccgtccat tggggatgaa agacaccacc
     5521 tacactccct caccggatca gtgccgccgt ttaatggttg ctgagcgcgg tgccagtccg
     5581 tgcaataaca cgctggcggc aattggcagc ggcggcgtct attccacgcc tggcgatatg
     5641 atgcgctgga tgcagcagta tttgtcatct gatttttatc agcgcagtaa ccaggcagat
     5701 cgcatgcaga cgttgattta tcagcgcgcg cagtttacga aagtgattgg catggatgtc
     5761 cccggcaaag cggatgcgct tggtttaggc tgggtatata tggccccgaa agagggtcgt
     5821 ccggggatta ttcagaagac aggtggtggt ggcggtttca ttacctatat ggcgatgatc
     5881 ccgcagaaga atattggcgc gtttgtggtg gtcactcgtt cgccgttgac gcgctttaaa
     5941 aatatgagcg atggcatcaa tgatctggtg accgagctaa gcgggaataa accgttggtt
     6001 atccccgcat cctgattaat actctgaagg caaacggttg atagcgacca gtttgccttt
     6061 attcatttcg atatagccgc ctttacgcaa agcagcgaga acttctgcga cgacagaacg
     6121 cgatatgcgt gtacgttgat ggatataatt catcacacca atacgtgatc gcaattcttc
     6181 attccagtca atcattgata ataatgttgc gcggatttgt tcgtaggaat tatgcccaat
     6241 gagctgcacg tcgcgtaatt ccagaattct attttgccag gctaaccagt aaaaagcgtc
     6301 tcgccagagt tgattttgtt caataagcgt aatggtttgt ttggctggta gatgatatcc
     6361 cgtacaattt ccttctgata ttaatttgta tggtatatcg tttttcatta aaccatcagc
     6421 cagccccata atataaggag cctgggtaat accgataagt acgttttctt ctctacgcag
     6481 agagataact ccctctaaaa taacgaaagt atcttcgtta ttgacatcac tggaaaatat
     6541 aacttgcttt tcattattaa actcgaagcg cgtaccgtat ctggacaaac atttatcgag
     6601 cttaccaaat tcctgaagag gtttaactac agataacatt tgcgcgtcct ttgcagtaat
     6661 gcccgtcaaa tccttgacgg gcattattta gattaaatta ccagtatttc ttcggagtga
     6721 agaatattac caggtatatt taacacccac gttcgcggac cagtcttgat ctacgtcacc
     6781 accaccgagg tagttagcat cggtataggc gctgaagttc ttggtgaagc tgaactgagt
     6841 acccagacca acacgtaccg cagacccttc agtaccgtta tcgatggaat caccgttcac
     6901 atcgttatcg ttgttagagt cgtcgtagac gtaagccagt ttgaagtacg gagtcagagc
     6961 ctgatcttcg ctgtaggtga aggtataacc tgcatctaca cccagttcat aacgcatgct
     7021 gtcgtaagac tgaccgtcaa ctttcatgtc gttgctcagc tggtagtcat caccagactg
     7081 gaacagacca gaaatgctgc cgtaaggagt cacgtaacca gcatcaccca gtttgaagtc
     7141 gtaaccggct ttcaaaccga agccccaagc gtcggagttg gtgctaccgt caacgtaagt
     7201 accgttgctc atggttgcag acaggtcgtt gttgaagtga gagtagctca agctaccatc
     7261 aacaaagacg ttgttcgcga agtgagcaga agagtagatg taggcagtct ggctgtcttg
     7321 atccacctga ccagaacggt cattcatgtc acctttagcg aagcctgcag ccgcaccgac
     7381 gatccactta gcgttgttac cgtcaatttt ggtatcaaca ccgaccatga tgccgttaac
     7441 atcctgatca tagttgatgg tgccgttgtc gccgttgaag ttaccaccga agtagcttac
     7501 ccatgcgccg ccgttatcag ccaggccatg acgagagttg gtcaaacgag taccaacggt
     7561 gtcttgttcc aggttccaga tattggtgtt cgcagatggg atgctcagcg ccatgttagc
     7621 gtagtcggtc aactccatct gttgcagaac aacggtgtta ccgcgctgtt cagcctgata
     7681 ggtgtatgca cccaggtcag ctttgttagc agcagagaag gtcgcgttgc tgtttttgtc
     7741 gttgacgtag atcagctcgt tgccgttgta gtcagcgata gaaccagcac cagtagcgtt
     7801 gtcgatacga actttgtagt taccggtcgc ggctgcaaca tggtcaccgt tgttatcaac
     7861 ttcgttctga ccagcttcga tagaagcagt gttgtcgtta tcgccgttac cgttaatagt
     7921 caggtgacca tcagagttca ttgcgattaa gccataaccg tagtttgact tggtagtatc
     7981 gttggtacga tcgttgacca gatcggtatc cattacgtaa tcactgctat ggatatcgaa
     8041 tacaccagcg tgaatgttgc cgttagtatc agtggtactg gtcagattca gggtatcggc
     8101 ctggaacggg ttagcatcga actgatcaac gttaatatcc aacagaccgt cgttagttac
     8161 ggtgatggta tttgcgtaca gtgcagcatc ttctaaaccc cagcccgcag aaacatcatc
     8221 agacatttta acggtactat tgtcgatggt cagggtatta gtcgctacat gaccgtcttc
     8281 accgatgttc aggttagaac cgccagtcag agagacagtg tcagagatca gacgagagtc
     8341 tgcaacatta acttgggaac cgctgttaat acttaaagta tcaatcagag aagattttgt
     8401 tgtatcccac tctgaaccac cgttcaaagt cacattaaac aaaccgctct ggtaaacttc
     8461 gtttcctacg atatggccat tttcgtcata ttgagtattg atgttatcaa caccgacagt
     8521 tgatgaaggc cacaggctgt tagctgcaat atcgagcaga gttgcagttg cttcagtacc
     8581 aacagcatat ccgtcgtaag aaccatcacc atcttcatca accatatgaa cagacattgc
     8641 agcgccaacc cacttgctgc cgttgttcag agtcacatcc atacggtctg tgccatccca
     8701 accgttggta tctacatcac catcagcatc gcgatagctg ttagcacctt gcgggaagaa
     8761 gttttcatcg aaattactgg agaaaacaac atcacccatc agtgttgagt tgtctaaagt
     8821 tactgtagtc tgcatcgcat tatcagcata cggatttgcg attgcggcaa ttgcaacatc
     8881 gtctgcagtc agcgtgttgc tatagttgct ggcattacca gtatggccaa accaaccagt
     8941 agtaccttca tcagtccatg aaccagaggt cacagtagaa tctttaacag taatagtgtt
     9001 attaaacact tcgctgctgt ttacgccagt gctgactgtg ttgtcgtcaa tatcttccca
     9061 ctcataaccc tgagtcagcg taatacctgc tacatgagag ttattagtga tggacagatc
     9121 aacttcctga tccagtgtaa tagcagtacc catatcgtaa acatctacaa catgagtgtc
     9181 agcggcatta ttataggtac cgttgtaagt ataatgctcg tagttgtcat cgatcgttga
     9241 attatcaacg ctcagtgtca gacgatcata aacataacca gtagcacggt catcagcaca
     9301 atcagtagtc atgcactcag aggtaatcat accgtgaata gtgctgtttt tgattgtcag
     9361 actattggcg ttggtattgg tagaaatacc gtcgtccaga tagtaggtag aaatcacgcc
     9421 gttaacggta gcgttattaa tgaccgggta gatatcgccg ttgtaatagc tatcagcggc
     9481 atagttgttc cagcctacat aaccatcata gtaaacgtca tcagcatagg tggcgtcgtt
     9541 gtagtgatgg aaagtattgt aagtttgacc agaaatatcg ttggtcgctg cattagcctg
     9601 agaggtgata gccagagtgc aagccaatgc taattgtgat actataagtt tctttttcca
     9661 ggagtgcatt tgtcatccct cctcagggac gtaataagtt gatccatcaa ttatcaatgc
     9721 atagaaaaca tcttatgttt cgcgttgacg attatgcggc gttatagagg aatggttcaa
     9781 ttattctttc cc
//
LOCUS       DAZKIP010000144         9730 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZKIP010000144 DAZKIP010000000
VERSION     DAZKIP010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.145"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..773
                     /locus_tag="ACHOUK_004025"
     CDS             <1..773
                     /locus_tag="ACHOUK_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9899369.1"
                     /translation="GWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKLEKTLLIN
                     QLASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGF
                     TASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIDIGLSGLLSPVLAIV
                     ALCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLLGAVGGFIAPILRVKAETLF
                     ASDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            799..1593
                     /gene="yfaU"
                     /locus_tag="ACHOUK_004026"
     CDS             799..1593
                     /gene="yfaU"
                     /locus_tag="ACHOUK_004026"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HFP9899370.1"
                     /translation="MINHPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLLIDG
                     EHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKPLIKQVLDIGAQTLLIPMVDTADQA
                     RQVVSATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDNLDE
                     ILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAVAPD
                     MAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(1634..2560)
                     /gene="rpnE"
                     /locus_tag="ACHOUK_004027"
     CDS             complement(1634..2560)
                     /gene="rpnE"
                     /locus_tag="ACHOUK_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFP9899371.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLEGLQEGMHEQAI
                     KIALRMLEQGFDRDLVLAATQLSEADLAANNH"
     gene            complement(2753..3943)
                     /gene="glpC"
                     /locus_tag="ACHOUK_004028"
     CDS             complement(2753..3943)
                     /gene="glpC"
                     /locus_tag="ACHOUK_004028"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFP9899372.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(3940..5199)
                     /gene="glpB"
                     /locus_tag="ACHOUK_004029"
     CDS             complement(3940..5199)
                     /gene="glpB"
                     /locus_tag="ACHOUK_004029"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFP9899373.1"
                     /translation="MRFDTIIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQAVTDIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELPHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLAVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDQTLRPSQAGQTIENLFTIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(5189..6817)
                     /gene="glpA"
                     /locus_tag="ACHOUK_004030"
     CDS             complement(5189..6817)
                     /gene="glpA"
                     /locus_tag="ACHOUK_004030"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFP9899374.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            7090..8448
                     /gene="glpT"
                     /locus_tag="ACHOUK_004031"
     CDS             7090..8448
                     /gene="glpT"
                     /locus_tag="ACHOUK_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFP9899375.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHQELLQKRN
                     GG"
     gene            8453..9529
                     /gene="glpQ"
                     /locus_tag="ACHOUK_004032"
     CDS             8453..9529
                     /gene="glpQ"
                     /locus_tag="ACHOUK_004032"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFP9899376.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
BASE COUNT         2226 a         2404 c         2686 g         2414 t
ORIGIN      
        1 ccggctggtt ctggatgttt gtgattgaag gattgttggc agtcggcgct ggggtattca
       61 cattcttttg gcttgatgac acaccggagc aggcacgttt tctgagtaaa ctagaaaaaa
      121 cgttgcttat caatcaactg gcaagtgaag aacaacagaa agtgacttct cggctgagcg
      181 atgcgctgcg taatgggcga gtctggcaac tggcgattat ctacctgacc attcaggtgg
      241 cggtttacgg attaattttc ttcctgccga cccaggttgc ggcattgctg ggaacaaaag
      301 tgggctttac agcgtcggtg gtcaccgcca ttccgtgggt tgcggccttg tttgggacct
      361 ggcttattcc gcgctattcc gataaaaccg gcgaacggcg taatgtcgca gcgctgacat
      421 tactggcggc gggcattgat attggtctgt ccgggctgct ttctccagta ctggcgatcg
      481 tagcgctgtg tgttgcagcc atcgggttta ttgccgtgca gccggtgttc tggacgatgc
      541 cgacacaact gctttccggt acggcgctgg ctgcggggat tggttttgta aacctgcttg
      601 gtgcagtggg cgggtttatt gccccgatcc tgcgcgtgaa agcagaaacg ttatttgcca
      661 gcgatgcggc gggattactg acgctggcag cggtggcggt catcggttcg ctgattattt
      721 tcactctgcg tgtaaatcgc accgttgcgc agaccgacgt ggcacatcat taaataggtt
      781 aaggaacacg atgaacgcat tattaaccat ccctttaaag aacgtttacg caagggcgaa
      841 gtgcaaattg gtctgtggtt aagctcaacg actgcctata tggcagaaat tgccgctact
      901 tccggttatg actggttgct gattgacggg gagcatgcgc caaacaccat tcaggatctt
      961 tatcaccagc tacaggcggt agccccctat gccagccaac ccgtgatccg tccggtggaa
     1021 ggcagtaaac cgctgattaa acaagtcctg gatattggcg cgcaaactct actgatcccg
     1081 atggtcgata ctgccgatca ggcacgtcag gtggtgtctg ccacgcgcta tcctccctac
     1141 ggtgaacgtg gtgttggggc cagtgtagca cgggctgcgc gctggggacg cattgagaat
     1201 tacatggcgc aagtcaacga ttcgctttgt ctgttagtgc aggtggaaag taaaacggca
     1261 ctggataacc tggacgagat cctcgacgtc gaagggattg atggcgtgtt tattggacct
     1321 gcggatcttt ctgcgtcgtt gggctacccg gataacgccg ggcacccgga agtgcagcga
     1381 attattgaaa ccagcattcg gcggatccgt gctgcgggta aagcggctgg ttttctggct
     1441 gtggctcctg atatggcgca gcaatgcctg gcgtggggag cgaactttgt cgctgttggc
     1501 gttgacacga tgctctacag cgatgccctg gatcaacgac tggcgatgtt taaatcaggc
     1561 aaaaatgggc cacgcataaa aggtagttat tgatatcaaa ggcccatggg gatcggctgt
     1621 gggactgtgt taattagtgg ttattcgctg ccagatcggc ttcgcttagc tgggtggccg
     1681 cgagcaccag gtcacgatca aagccctgtt ccagcatgcg caaggcaatt ttaatagctt
     1741 gttcatgcat gccttcctgc aaaccttcta atttaccttc ctgccaacca atttgatgtc
     1801 cttcctgccg tagccgttca gcaatagtca ttaatctctc cttttgtagt ggtgaacgtt
     1861 cggcaatctc ctgaataaaa cgggtgaaac gggaggtatc gccgcattgc agcagataat
     1921 taaacagtgt ttgtagctgg ctgtcattag tgaagcctct aaccaaaagc gtggtgatcc
     1981 tgtcgaccat accgattaaa tcgcggtcgc gaatatgctt ttgaatcagt tcaagcagag
     2041 ctatacgccg atgttgcatg atctcatcgt caggtacgat ggtaatatcc accaacggaa
     2101 aagcttcggt gtacaactgc cgggcaagtt gcggatcgtc aaactcatcc agccagttga
     2161 gtgagtacgg gtagggcgag gtttcgccat gataaaacag caacggcacc accagcggga
     2221 ctctgtcata gcctttgtcc aggtgacgct gcatggcggc agtggcatag cgcattagcc
     2281 gaaaagccat attcttttct gcagagcttt gatgttcaat cacgcagtag atatagccgt
     2341 caccgtcgct ggtttccacg gaccacaaaa catccgagta gtaagcgcgt aaactttttt
     2401 caataaaact agtgggttcc aggcgtaagg tttgcaggtt gcaaagcttg cgcagtggtt
     2461 ctggtaaatg tatttcgaga aaatcccgtg cggtttcggg tgtgaacata aaggttttaa
     2521 atactgcatc atgcggggag gaggttgttg attcggtcat ggttgtccgt cacctgatat
     2581 ttatgatgac gcgacattac ggttatccca ggcctttgac atctgccagt ttcagccttt
     2641 gcgagacgtt ttcagaggaa ttttcactgc gttgcaacgc atacattggg cacggaatcg
     2701 tgtacgcaaa aatgcccggt aaccacatgc ttaccgggca tcagaaagga gtttaagcca
     2761 gcgcctgggc cagtagcgta atcggatgtt cgcagcgaag acttgtggac atctcaatct
     2821 gccatttaca ggtttcgcag tcggtgacca ccagatctgc gccgctttct tctatctggc
     2881 ggaacagtgg tgcgccgatg gcttgtgagg tggggtagtt ctcttttttg aaaccgtaag
     2941 tacccgcaat accgcagcac tgggaatcca gcaccgttaa ctcaagcccc gggatgttac
     3001 gcaacagctc cagggtgtag agcgtccagc ccattttttc catatggcac ggagtgtgat
     3061 aaaccacttt cagcggcagc ggtttcagcg gtaacgtttt gccttcgtcc agcttgcgcc
     3121 acagccagcg ggtcgccagt tcgatatgat cgcgcaagcc tttgttgtcg acattcagca
     3181 cttccgggta ttcgtcgcgc agggcaaatg tacaggttga ggaggtggca atcaccggaa
     3241 tgccttttac tcccacagct tcgcggatcg actctacatt cgtaattgcc tgtttgcgtg
     3301 ctttatcggt aaagccgttg gcgatcagcg gtacgccgca gcatttttct ttgctgagca
     3361 gttgtacacc ggtacccatt gcgttgagca ctttaattaa atctttacct aactgcggat
     3421 ggttgtagtt aacgaagcag ccgtgaaaga aagcgacctg gtctttatac tgcgcttgct
     3481 gagccgccac gctgcgatac cagcgacgaa acgtgccgaa ggagtatttc ggtagcgtgc
     3541 ggcgatgatc gatttttaac gccgcatcaa gcaactgccg caccggtttc agcgaggtag
     3601 cggtgttgac gattggtgcg aacggcgtgg aaacgctacc catcaggtcg gtatggctta
     3661 aaacaaaatt gcgcagcgac gggcgcgtgg tgtcatattt cgcccgtgcg cgctggatga
     3721 tatcgccaat cttcacatcg gacgggcagg cgacttcgca acgtttgcag ttgatgcaat
     3781 atttcagtgc ctcgtcatac agtgcgccat cttttaaacg cagacgctcg ccatccggcc
     3841 cggcttgttt tggccctgga taaccgggat tcacccggct cactgggcag gcggtggtgc
     3901 agacggtgca cttaatgcag ttttcgaagc tggtgtcatt cattgttggc ctcctgcgcg
     3961 ttgggcaatc tgttgagcgg catgtaaagc actgacggca caaacaccgc cgccgcatcc
     4021 ctgggcgatg ggatcaaatc cgcccagcac cgaaccgatg gtaaacaggt tttcgatggt
     4081 ttgccctgcc tgcgacgggc gtagcgtctg atcagtggtt acaccgaact gctgccacgg
     4141 ttgcggcgca aaaaaatctc ccttatacca ttcaccccgc gtggcggttt gtagcacatc
     4201 aaggccgaga atcggctctc gaatgccgtt acgttccgct accagtccgc cactaaagaa
     4261 actgccgctg gcgagaaccg cgaaacgtgg acgcagcggg atatcggcgt gattgcgggt
     4321 ccagatttca ttcactacgc catttttaca ggtcactttt ttcacttcat cgcccggcat
     4381 ccacacgcca ccctgacgca caaactggcg ctgtaactgg ttttgcagac gaatgcccag
     4441 cacggaaggc ggcagcgttg gcaaaagcat cagtgaacaa ggtagttttt cattcaacca
     4501 acgccacagt ttgtcatcag ccaggccgaa gcaggcgggc atcaggatca tttcgcaggt
     4561 attggcgaca ggaataagcg catcaagtaa cagcggccag ttttcttcat tatcaaggaa
     4621 acgggcgata ttcaccgcgc ggaactcggt ggcgttattg cgcaggacat ccagttccgg
     4681 cagctctatt tctgcggttt caacggcaag gtcgagttca cgcaacgaag ctgccgcaag
     4741 gtgcgcctga aaatccatca gtccgctaat tcccactaca catattttct tcgcgggcag
     4801 cggccagacg gggacttctg gcgaacttag ccaggtagag cgcagagtgc cgagcggcgt
     4861 aatccgctgg tgaggcagtt ctacgctgcc ctgcaattgc gcaccgcttt cagcgattaa
     4921 tgcctgcgcc tggcaagcga gatcgagcac gcgttgtggc ccgagaagag agtaaggatg
     4981 tgcaggtgcc tgctgacgca aagattccag tccactgtga atgtctgtca cagcttgacc
     5041 atctggcaga tggctcagca aatccagcga accggatgag aaatgcagcg cgctttgacc
     5101 acgcgtgaca atggcacagc gcaggccgtg tttttgcagc tgcaggccac agagtaatcc
     5161 ggcgaggccg ccgcccataa tgatagtatc aaagcgcatc tttctgctcc ttctccagac
     5221 cacacagtcc ctgataaacc cagcgggtaa attcgctttc gcgcagtgca tctccccagg
     5281 cgatgggttg cacgcctttc cagcgttcgt taaggaaggt ggaaagttgc tcgatagatt
     5341 gcgcggacgt cgtgacgtta aaacgttgca gcagtccagc agcgcggcag gcgcagagtt
     5401 cgccctggca ggtacccatc cccacacggg tacgacggcg taaatccagc aggctattaa
     5461 cgtttaaatt ttctaccgcg tactgcactt cacccgcagt taccgcttcg cactcacata
     5521 ccaggctacg gtgcagacgg ccttcgctca gccaggcagg cgtgcgatcg ccatgacgat
     5581 aaaccgcaga accgcgcagc ggggcaggca gggagatgac tttacgcaag gtaacttcag
     5641 cgggttcctg tgaacccgga agtgccagat cggcggtcgt acaggggcgc gtgttgccca
     5701 gtttgcggca taccgcgtcg gtagcccatt cagccatcag ccgataggtc atcagtttgc
     5761 caccggtgat ggtgataaat ccgtccagac catcgcgttc agcatggtcg agcagcacga
     5821 tgccacggct gacgttacgt ccgctcgggt cgtcatcgct ggcaaccagc gggcgcacgc
     5881 cagaataggc ccgcaaaatg cgcgttttcg ccatcacggg ggccagtttt tccccttcac
     5941 gcagcagaat atcaacctct tctgccgtca ctcgattatc gtcaatctcg ttgtaatcaa
     6001 tacgtaaaga ggtggtgcca atcagcgaaa tggtatcgcc aggcaccaga atatcggcgt
     6061 cggaaggttt acggcagcgg ttgatcacat gctgattaat gcggtgatcc atgatcagca
     6121 gcgatccttt cgccgggaac atgcgaatgc gcagatcggc atattcggca atgtgttgcc
     6181 cccagatccc agcggcatta accacgacag gtgcatgaag ggcctgagtt tcgccggtga
     6241 gatggttacg tacacgaaca ccgcacaccg tcgcgccttc acgaatcagc cccgtgactt
     6301 catgagcggt aaggataaca gcaccgtgtt ctttggcatc cagcatgttt gctgcggtca
     6361 gacgaaatgg atcaacggtg ccatccggaa ctttcaccgc gccaatcagt gccgggttaa
     6421 cggcaggttc gataatgcgc gcttgctgcg ggtctatagc ttctgcgctg atccctgctt
     6481 cttcgcaggc gcgaataaaa gtggcctgga aggagaggtc atcttccggc agggtgataa
     6541 acaggccgtt ggttggttca acgcagtgac gtgcaatgcg tttcaggatc tggttttcac
     6601 taatgcattc gcgggccgat tccgcatcgg ttaccgcata gcgcgcaccg ctgtgcagca
     6661 ggccgtggtt acgcccggtg gcaccggttg cgatgtcgtg gcgctcaacc aaaatcacgc
     6721 gcagcccgcg cagggcacag tcgcgggcaa ttccggctcc cgttgcgccg ccgccaatga
     6781 taatcacgtc acttgattgc gagtcgcgag ttttcattgt tatccctctg aagttcgttt
     6841 tttaccattt agccatagta aaaacatgag ttgtttgatt tcgcgcatat tcgctcataa
     6901 ttcgaaagtg agacgtgatt tcatgcgtta ttttgaacat tttgtaaatc ttatttaata
     6961 atgtgtgcgg caattcacat ttaatttatg aatgttttct taacatcgcg gcaactcaag
     7021 aaacggcagg ttctctcact gaatcaggct gttaatcata aataagacca cgggccacgg
     7081 aggctttcaa tgttgagtat ttttaaacca gcgccacaca aagcgcgctt acctgccgcg
     7141 gagatcgatc cgacttatcg tcgattgcgc tggcaaattt tcctggggat attctttggc
     7201 tatgctgctt actatttggt tcgtaagaac tttgcgctcg ctatgcctta cctggtggag
     7261 cagggattct cacgcggtga tttaggtttt gcgctttctg ggatctcgat cgcctatgga
     7321 ttttcgaaat tcatcatggg ttcggtatcg gatcgctcga atccgcgcgt tttcctgccc
     7381 gcaggtttga ttctggcggc ggcagtgatg ttgtttatgg gctttgtgcc atgggcgacg
     7441 tcgagcattg cggtgatgtt tgtactgttg ttcctctgcg gttggttcca ggggatgggg
     7501 tggccgccat gcggtcgtac tatggtgcac tggtggtcgc agaaagaacg tggcggcatt
     7561 gtgtcagtgt ggaactgtgc gcacaacgtc ggtggtggta ttccgccgct gctgttcctg
     7621 ctggggatgg cctggttcaa tgactggcac gcggcgctct atatgcccgc tttctgcgcc
     7681 attctggtgg cattattcgc ctttgcgatg atgcgcgata ccccgcaatc ctgtggcttg
     7741 ccgccgatcg aagagtacaa aaatgattat ccggacgact ataacgaaaa agcggaacag
     7801 gagctgaccg cgaagcaaat cttcatgcag tacgtgctgc cgaacaaact gttgtggtat
     7861 atcgccatcg ccaacgtgtt cgtttatctg ctgcgttacg gcatcctcga ctggtcaccg
     7921 acttatctga aagaggttaa gcatttcgcg ctggataaat cctcctgggc ctacttcttc
     7981 tatgagtatg cgggtattcc gggcaccctg ctgtgcggct ggatgtcgga taaagtcttc
     8041 cgtggcaacc gtggggcaac cggcgttttc tttatgacac tggtgaccat cgcgactatc
     8101 gtttactgga tgaacccggc aggtaaccca accgtcgata tgatttgtat gattgttatc
     8161 ggcttcctga tctacggccc tgtgatgctg atcggtctgc atgcgctgga actggcaccg
     8221 aaaaaagcgg caggaacggc agcgggcttt accggtctgt ttggttacct gggcggttcg
     8281 gtggcggcga gcgcgattgt tggctacacc gtggacttct tcggctggga tggcggcttt
     8341 atggtaatga ttggcggcag cattctagcg gttatcttgt tgattgttgt gatgattggc
     8401 gaaaaacgtc gccatcagga attactgcaa aaacgcaacg gaggctaatg gcatgaaatt
     8461 gacgctgaaa aaccttagca tggcgatcat gatgagcact atagtcatgg gaagcagtgc
     8521 aatggcggcg gacagcaacg aaaaaatagt catcgcccat cgcggtgcca gtggatattt
     8581 gccggagcat acgctgccag caaaagcgat ggcgtatgcg cagggagcgg attatctgga
     8641 acaggatttg gtgatgacca aagacgacca tctggttgtt ctgcatgacc attacctcga
     8701 tcgtgttact gatgttgccg atcgtttccc ggatcgggcg cgcaaagacg gtcgttacta
     8761 cgcgatagat ttcacgctgg atgaaattaa gtcgttgaaa tttaccgaag gtttcgatat
     8821 tgaaaacggt aaaaaagtgc agacttatcc agggcgtttc ccaatgggta agtccgactt
     8881 ccgggtgcac acctttgaag aagagattga atttgttcag gggttaaatc actctaccgg
     8941 gaaaaatatc ggtatctatc cagaaatcaa agcgccgtgg ttccatcatc aggaagggaa
     9001 ggatattgcg gcaaaaacgc tggaagtgct gaagaaatat ggttacaccg gtaaagacga
     9061 taaagtttat ttgcaatgtt ttgatgctga tgagctgaag cgtattaaga atgagctgga
     9121 acccaaaatg ggcatggatc tcaatctggt acagctgatt gcctataccg actggaatga
     9181 aacgcagcag aaacagccgg acggaagctg ggttaattac aactacgact ggatgtttaa
     9241 gccgggtgcc atgaaacagg tggcggaata tgcagatggt attggtccgg attaccatat
     9301 gttgattgag gagacatcgc agccgggtaa tatcaaactc actggcatgg tgcaagatgc
     9361 tcagcagaac aagctggtag tgcatcctta taccgtgcgg tcagataaac tgcctgaata
     9421 cacaactgat gtgaatcagt tatatgatgc tctgtataac aaagcgggtg taaatgggtt
     9481 gtttactgat ttccctgata aagcggttaa attccttaat aaagagtaat gctgctactg
     9541 caggatggcg ttcatgcgcc atcctctgct aaagcgaaat aatgctacta ccggttattc
     9601 ttttcattaa ccggtgcgtc ctgtaaaaaa gatgccaaca taattttcgt aaaactcatt
     9661 cgttaaaaaa gctaacactg ggcattgtta ttctttaaac agtatgaata ggtctcaata
     9721 ataaattgta
//
LOCUS       DAZKIP010000145         9727 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZKIP010000145 DAZKIP010000000
VERSION     DAZKIP010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.146"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            230..649
                     /locus_tag="ACHOUK_004033"
     CDS             230..649
                     /locus_tag="ACHOUK_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFP9899377.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            649..1917
                     /gene="umuC"
                     /locus_tag="ACHOUK_004034"
     CDS             649..1917
                     /gene="umuC"
                     /locus_tag="ACHOUK_004034"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFP9899378.1"
                     /translation="MFALCDVNAFYASCETVFRPELWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(1963..2493)
                     /gene="dsbB"
                     /locus_tag="ACHOUK_004035"
     CDS             complement(1963..2493)
                     /gene="dsbB"
                     /locus_tag="ACHOUK_004035"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFP9899379.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(2639..4180)
                     /gene="nhaB"
                     /locus_tag="ACHOUK_004036"
     CDS             complement(2639..4180)
                     /gene="nhaB"
                     /locus_tag="ACHOUK_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFP9899380.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4402..5121
                     /gene="fadR"
                     /locus_tag="ACHOUK_004037"
     CDS             4402..5121
                     /gene="fadR"
                     /locus_tag="ACHOUK_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFP9899381.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(5173..6705)
                     /locus_tag="ACHOUK_004038"
     CDS             complement(5173..6705)
                     /locus_tag="ACHOUK_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFP9899382.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            7068..8366
                     /gene="dadA"
                     /locus_tag="ACHOUK_004039"
     CDS             7068..8366
                     /gene="dadA"
                     /locus_tag="ACHOUK_004039"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFP9899383.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            8376..9446
                     /gene="dadX"
                     /locus_tag="ACHOUK_004040"
     CDS             8376..9446
                     /gene="dadX"
                     /locus_tag="ACHOUK_004040"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFP9899384.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEMYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
BASE COUNT         2421 a         2397 c         2605 g         2304 t
ORIGIN      
        1 atgtttaatg tcattatggc gaatgcttct attctatttt ttagccgggt gatatttttc
       61 atttctgctg gatgagcgtc gtcgccagaa ggccgcgtga gcacaggata agagaacgaa
      121 aaatcagcag tctatgcagc gacaaatatt gatagcctga atcagtattg atctgctggc
      181 aagaacagac tactgtatat aaaaacagta taacttcagg cagattatta tgttgtttat
      241 caagcctgcg gatctccgcg aaattgtgac ttttccgcta tttagcgatc ttgttcagtg
      301 tggctttcct tcaccggccg cagattacgt tgaacagcgc atcgatctga atcaactgtt
      361 gatccagcat cccagcgcga cttacttcgt caaagcaagt ggtgattcta tgattgatgg
      421 tggaattagt gacggtgatt tactgattgt cgatagcgct attaccgcca gccatggtga
      481 tattgtcatc gctgctgttg acggcgagtt tacggtgaaa aaattgcaac tacgcccgac
      541 ggtacagctt attcccatga acagcgcgta ctcgcccatt accatcagta gtgaagatac
      601 gctggatgtc tttggtgtgg tgatccacgt cgttaaggcg atgcgctgat gtttgccctc
      661 tgtgatgtaa acgcgtttta tgccagctgt gagacggtgt ttcgccctga gttatggggt
      721 aaaccggtgg ttgtgctatc gaataatgac ggttgcgtta tcgcccgaaa cgctgaggca
      781 aaggcgcttg gcgttaaaat gggcgatccc tggttcaaac aaaaagatct gtttcgtcgc
      841 tgtggcgtgg tttgctttag cagcaattat gagctttacg cagacatgag caatcgggtg
      901 atgtcgacgc tggaagaact atcgccccgc gtcgagattt acagtattga tgaggcattc
      961 tgcgatctga caggtgtgcg taattgtcgc gatctgactg actttggcag agaaattcgc
     1021 gcaacggtgc tacaacgtac ccatcttact gttggtgtgg ggatcgccca gaccaaaacg
     1081 ctggctaagc ttgccaatca tgcggcaaaa aaatggcagc ggcagacggg tggggtggtg
     1141 gatttatcaa atctggaacg ccagcgtaaa ttaatgtctg ctctccccgt ggatgacgtc
     1201 tgggggattg gacggcggat cagcaaaaaa ctggacgcga tggggatcaa aaccgttctc
     1261 gatttggcgg atacagatat ccggtttatc cgtaaacatt ttaatgtcgt gctcgaaaga
     1321 acggtgcgtg aactgcgcgg tgaaccctgt ttgcaactgg aagaatttgc accgacaaag
     1381 caggaaatta tctgttcccg ctcgtttggt gaacgcatca cggattatac gtcgatgcgg
     1441 caggccattt gtagttacgc tgcccgggcg gcggaaaaac ttcgcagtga gcatcaatat
     1501 tgtcggttta tctccacatt tattaagacg tcaccatttg cgctcaatga accttattac
     1561 ggcaatagcg cgtcggtaaa actgctgacg cccactcagg acagcaggga tatcattaac
     1621 gccgctacgc gatctctgga tgccatctgg caagcgggcc atcgctacca aaaagcgggc
     1681 gtgatgctgg gggatttctt cagtcaggga gtcgcgcagc tcaatttatt cgatgacaac
     1741 gctccgcgcc ccgggagtga gcaattgatg gcggtaatgg atacactgaa tgctaaagag
     1801 ggcagaggaa cactctattt tgccgggcag gggatccagc aacaatggca gatgaagcga
     1861 gccatgcttt caccacgtta tacaacgcga agttctgatt tactgcgggt caaataaaca
     1921 cagcggcagg aaaaaagcgc tcccgcagga gcgccgaatg gattagcgac cgaacaggtc
     1981 acgttttttc gctttaaacg gctgggaaat caccaccagc accgcgacaa tcaggtaagc
     2041 gataaaaata ccgagcagcc actgcggcat ttccagacct aaaaaatccc actgacgctc
     2101 ggcgcaatcg ccagaggcga caaacacttg cggcacccat ttatccagcg gcagccattc
     2161 cgggaaacga accataaaat cacaggtggc aaacggcgaa ggatagagct gaagcatggt
     2221 gtgctcgtaa gttaactgca caccgcggaa cgcactatac aaccagataa ccatcgctac
     2281 ataacgcagt ggcgtttttg gggcgatcgc gccaatcagc gcagcaccca gaacgccgaa
     2341 taatgcgcag cgttcataaa tacagagcac gcaaggtttc agtaacatca catgctggaa
     2401 ccacagcgcc gtcagttcca gtgccagagc agtaaacgcc atcaacagcc acgcgccccg
     2461 gccttgtgaa cattggttca aaaatcgtaa cataatcatt tccctgcaat atgcatagag
     2521 tgcgcagttt aaaccaattc attcgctgcg ccaccaggag gcagcaaaaa taggacgcaa
     2581 ttggacaatt atcgggcgaa aaggcaaacc gggcatttat atgcccggta agttgttatc
     2641 aaagcgttgc tatccagccc atttgcataa accattcggt tacaggggca agcgtaaact
     2701 cgacgcagag cagtccgacg agtgtcagga caagggtgta aggcagggcc atccacacca
     2761 tgcggccata agagaggcga atcaatggcg cgagtgcaga ggtcagcagg aacaggaacg
     2821 cagcctgacc gttcggcgta gcgacagagg gcagattggt accggtatta atggcgaccg
     2881 ccagcagctc gtattgcttc aacgtgatag cgccactttc cattgccgct ttcgcttcgt
     2941 tgatataaat cgtccccacg aagacgttat ccgaaatgga tgacagcaga ccgttgaaaa
     3001 tatagaacag cgacagctga gcatgttccg atgcctgcaa cacaaactga ataatcggcg
     3061 aaaacagttg ttggtcgata atcaccgcga ctaccgagaa aaacaccgtc aacagtgcgg
     3121 tgaatggcag agattcggtg aaggctttac cgatagcatg ttcatcggtg acaccggtca
     3181 atgatgttgc cagaataatg actgacaaac caatcaagcc aacttccgcc agatgcaacg
     3241 ccagcgcagt caccagccag acgccaataa tcgcctggac aatcagacgg attttatcct
     3301 gacgggtacg ctggtggcgg ctttgatcgt caaactgttg caacacttcg cggactttct
     3361 ccggcagcgt ttcaccgtag ccaaaccaac gcagcttctc taccagcagg caggttaaca
     3421 ggccacaaat cagaaccgga acggtcaccg gcgacatgcg caggaagaaa tcgccaaaat
     3481 gccagccagc cgctttagcg atgatcaggt tctgtggttc gcccaccatg gtcattacgc
     3541 cgcctaatgc ggtgccgaca ccggcatgca tcatcaggct acgcagaaag ccacggaact
     3601 gttccagaac cactttgtaa tgcttgtcga tatgactatc gtcttgcagg tcggtgtctt
     3661 cggtacggga agaggctacg cgatgataaa taccataaaa accgacggca acgctgatca
     3721 ccaccgccac gacggttaag gcatcgagga acgcggagag gaacgctgcc gctacgcaaa
     3781 aagagagcga cagcagcatt ttggagcgaa tgcttaacag caaacgggta aatatgaaca
     3841 gcaatagctg tttcataaaa tagatacccg ccaccataaa catcagtaac agcaagactt
     3901 caagatttgc cgccacctct tcacggacgt gttccgcgct ggtcatgccg atgaataccg
     3961 cttcgatagc caacaggcca ccggggagca gcgggtagca tttcagggcc atcgccagag
     4021 tgaaaataaa ttccgctacc agcaaccagc cagcgacgaa agggctgatg aggaaaatta
     4081 acggatttac gattaagaaa atgatgaggg cgagtttgta ccagtcgggg gactggccca
     4141 aaaaattgcg ccatagcgcg cggccccagg agatctccat gatggtttcc cttaccttac
     4201 aaataatcaa tgatgttttt atgtttaaac gcaaagctta acggtcaggc aggagtgagg
     4261 caagtcttga tagtcaaggg gaaagagatg cggaaaatga agccttgatc ccttttttct
     4321 tctttttgtc tgctatcagc gtagttagcc ctctggtatg atgagtccaa ctttgttttg
     4381 ctgtgttatg gaaatctcac tatggtcatt aaggcgcaaa gcccggcggg tttcgcggaa
     4441 gagtacatta ttgaaagtat ctggaataac cgcttccctc ccgggactat tttgcccgca
     4501 gaacgtgaac tttcagaatt aattggcgta acgcgtacta cgttacgtga agtgttacag
     4561 cgtctggcac gagatggctg gttgaccatt caacatggca agccgacgaa ggtgaataat
     4621 ttctgggaaa cctctggttt aaatatcctt gaaacactgg cgcgactgga tcacgaaagt
     4681 gtgccgcagc ttattgataa tttgctgtcg gtgcgtacca atatttcaac tatttttatt
     4741 cgcaccgcgt ttcgtcagca tcccgataaa gcgcaggaag tgctggctac cgctaatgaa
     4801 gtggccgatc acgccgatgc ctttgccgag ctggattaca acatattccg cggcctggcg
     4861 tttgcttccg gcaacccgat ttacggtctg atccttaacg ggatgaaggg actgtatacg
     4921 cgcattggtc gtcactattt cgccaatccg gaagcgcgca gtctggcgct gggcttctac
     4981 cacaaactgt cggcgttgtg cagtgaaggc gcgcacgatc aggtgtacga aacagtgcgt
     5041 cgctatgggc atgagagtgg cgagatttgg caccggatgc agaaaaatct gccgggtgat
     5101 ttagccattc aggggcgata atcccttccg tttaaaaaac aaacccctca aacgaggggt
     5161 tttttgttgt ttttacagat ttcccattct cggcgggcaa cgttccagca actcgacgct
     5221 gccgtcttcg ttttgctgtt ccagcatcac atcaaatccc cacaggcgat gcacatgctt
     5281 cagtacttct ttgcgccccc gatccagcgg tgcgcgatta tgtggaatgt aacgcagcgt
     5341 cagcgaacgg tcgccgcgca aatccacgtt ccagatctga atattcggct ccagattact
     5401 taagttatat tgcgacgata gccggttacg gatctcccga taaccttctt cattatgaat
     5461 agcggaaatc tccagataat tatgccgatc atcatccagc acggtgaaga agcggaaatc
     5521 acgcatcact ttcggcgaca ggaactggct gataaaactc tcatctttga aatcacgcat
     5581 cgcgaaatgc agcgtttcca gccagtcgga accggcgata tccgggaacc agtatttgtc
     5641 ttcttccgtt ggcgactgac aaatccgttt aatatcctgg aacatggcga acccgagggc
     5701 atacgggttg atgccgctgt accacgggct gttatagggg ggctggaaga ccacattggt
     5761 gtggctgtgc aaaaactcca gcataaaacg ttccgttact ttcccttcat catacagatg
     5821 gttaaggatg gtgtagtgcc agaaggtggc ccagccttcg ttcatcacct gagtctgttt
     5881 ttgcggataa aaatactggc tcaccttacg cacaatacgc aggatttcac gctgccatga
     5941 ttccagcagt ggcgcatttt tctccataaa atagagcagg ttttcttgtg gttcggacgg
     6001 ataacggcgc gcttcagcaa ccgttttctc ttcctcgcgc ttcggcaggg tacgccagag
     6061 catattgacc tgactttgca aatactcttc gcgacttttc tgccgggctt tctcttcttg
     6121 cagcgagatt ttttgcgggc gtttgtaccg gtccacgccg tagttcatca gcgcgtggca
     6181 cgagtccaga agccgttcta cttcatcaac gccataacgt tcttcgcact cggtaatata
     6241 tttacgggca aaaatcagat aatcgacaat cgaactggcg tcggtccagc tacggaataa
     6301 gtaattgttt ttgaagaaag agttatgccc atagcaggca tgagccatca ccagcgcttg
     6361 catggtaatg gtgttctctt ccatcaggta agcgatacac gggttagagt taatgacgat
     6421 ttcataggcc agtccttgct gaccgtgctt atacagccgt tcagtctcga taaacttttt
     6481 accgaatgac cagtgcggat agttaattgg catgccgacg ctggagtagg catccatcat
     6541 ctgttctgag gttatcactt caatctggtg cgggtaggta tccagccggt agagtttcgc
     6601 cacccggtct atctctgcca gataaacatc cagcaggtcg aacgtccagt cgggtccatc
     6661 gctcaaacgt gtggtgtcct tattcataga atcgatcgtc gccatacgcg cacctcattg
     6721 ttgtcggcgc tctctgtgtg gagcacctca ttgttgtcgg cgctctctgt gtggagcacc
     6781 tcatttcaag catagaacac ctgttaaaaa ccgcgtcgcc ggagaatttt tttctttgcg
     6841 atttcttatt atcagagtgc cactaatccg tttctgaacg gaattttatg ctggataaaa
     6901 agggcgttca gcaggagata ctaaagacgc catattgccg cagagtcagg gagatgtgag
     6961 ccagctcacc ataaaaaagc cgtatgttga ataatatttt caactgagtt atcaagatgt
     7021 aattagatta ttattctttt actgtatcta ccgttatcgg agtggctatg cgagttgtca
     7081 tactgggaag tggtgtggta ggcgttgcca gcgcctggta cttaaatcag gcaggacatg
     7141 aggtcaccgt cattgatcgg gagccggggg ccgccctgga aaccagtgcc gcgaatgccg
     7201 ggcaaatctc ccccggatat gctgcaccgt gggcggcacc aggtgtgcct ttaaaagcga
     7261 ttaaatggat gttccagcgc catgcgccgc tggcggttcg tctcgacggt acgcagttcc
     7321 agttgaaatg gatgtggcaa atgttacgta actgcgacac cagccactac atggaaaaca
     7381 aagggcggat ggtgcgtctg gcggaataca gccgtgattg cctgaaagca ttgcgcgccg
     7441 aaaccaatat tcagtatgaa gggcgtcagg gtgggacgct gcaactgttc cgtaccgaac
     7501 aacagtatga aaatgcgacc cgcgatatcg ccgtgctgga agatgccggc gtaccgtatc
     7561 agctgctgga atccagccgc ctggcggaag tggagcccgc gctggcagaa gtagcgcaca
     7621 aactgacggg tggtctgcaa ttgcccaatg atgaaaccgg agactgccag ctgtttaccc
     7681 agaatctggc gcggatggcg gagcaggcgg gggttaaatt ccgctttaat acgcccgttg
     7741 accaactgct ttgcgacggc gagcaaatct acggcgtgaa gtgtggcgat gaagtgatta
     7801 aggccgatgc gtatgtgatg gcgtttggtt cttactcgac ggcgatgctc aaaggcattg
     7861 ttgatattcc ggtgtatccg ctgaaaggct actcgctgac cattccaatt gcgcaggaag
     7921 atggtgcgcc ggtatccacc attcttgatg aaacctacaa aatcgccatt acccgtttcg
     7981 ataaccgcat tcgtgttggc ggaatggcgg aaattgttgg ttttaatacc gagctgttgc
     8041 aaccgcgtcg tgaaacgctg gagatggtgg ttcgcgatct ctatccacgc ggcggtcatg
     8101 tcgagcaggc gactttctgg acgggtctgc gcccgatgac gccagacggc acgccggttg
     8161 tcgggcgtac acgctttaaa aatctgtggc taaataccgg tcacggcacg ctcggctgga
     8221 cgatggcttg cggttccggt cagttgttaa gcgatctgct ctctggtcgc acgccagcga
     8281 tcccatatga ggatctaagc gtagcgcgct acagccgtgg atttacgcca tcacgtccgg
     8341 gccatttaca tggcgcacac agctaaggaa acgagatgac ccgtccgata caggccagcc
     8401 tcgatctgca ggcattaaaa cagaatctgt ccattgtccg ccaggccgcg ccgcacgcgc
     8461 gcgtctggtc ggtggtaaaa gcgaacgctt acgggcacgg tattgagcgt atctggagcg
     8521 cgctcggggc taccgatggc tttgcattac ttaacctgga agaagcaata acgttacgtg
     8581 agcgcggctg gaaaggaccg atcctgatgc tggaaggatt tttccatgct caggatctgg
     8641 aaatgtatga ccagcaccgc ctgaccacct gcgtacacag caactggcag ctcaaagcac
     8701 tgcaaaatgc gcggctaaaa gcaccgttgg atatttatct taaagtgaac agtgggatga
     8761 atcggttggg cttccagccc gatcgcgtgc ttaccgtctg gcagcagttg cgggcgatgg
     8821 cgaatgttgg cgaaatgacc ctgatgtcgc attttgccga ggcggaacat cctgatggaa
     8881 tttccggcgc gatggcgcgt attgagcagg cggcggaagg gctggagtgt cggcgttcgt
     8941 tgtccaattc ggcggcgact ctgtggcacc cagaagcgca ttttgactgg gttcggcctg
     9001 gcattatttt gtatggcgct tcgccgtccg gtcagtggcg tgatatcgcc aatactggat
     9061 tacgtccggt gatgacgcta agcagtgaga ttattggtgt ccagacgcta aaagcgggcg
     9121 agcgtgtggg ctacggcggt cgttatactg cgcgcgatga gcagcgaatc ggcattgttg
     9181 ccgcaggata cgccgacggt tatccgcgcc acgcgcctac cggtacccct gttttagtgg
     9241 acggcgtgcg caccatgacg gtggggacag tctcgatgga tatgctggcg gtcgatttaa
     9301 cgccttgccc gcaggcgggt attggtacgc cagttgagct gtggggcaag gagatcaaaa
     9361 ttgatgatgt cgccgccgct gccggaacgg tgggctatga gttgatgtgc gcgctggcgt
     9421 tacgcgtccc ggttgtgacg gtgtaacttg ttgtaagtcg gatcggaggc aacgtcttct
     9481 ggttgcaaaa aaaatcatcc atccggctgg tcagcaactg tagttgttaa tgtgacagag
     9541 ccattgccca tgatagtgcc cattaaaagg atggacacta tttccccgga acctgaactc
     9601 accgcacagg cgttctacat aaaacgctta cgcttcattg ttgactcgaa ctcgacttca
     9661 gataaatcac gcttcaccct tgatgcagat tcgggttcat gttcagaatg ttgattaaca
     9721 ggctgcg
//
LOCUS       DAZKIP010000146         9604 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZKIP010000146 DAZKIP010000000
VERSION     DAZKIP010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.147"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            53..2314
                     /gene="ybhJ"
                     /locus_tag="ACHOUK_004041"
     CDS             53..2314
                     /gene="ybhJ"
                     /locus_tag="ACHOUK_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFP9899385.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGAAQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     VEAPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(2455..3738)
                     /gene="ybhC"
                     /locus_tag="ACHOUK_004042"
     CDS             complement(2455..3738)
                     /gene="ybhC"
                     /locus_tag="ACHOUK_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFP9899386.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWGDAVISNRPFAGNTGSVD
                     DSDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(3873..4943)
                     /locus_tag="ACHOUK_004043"
     CDS             complement(3873..4943)
                     /locus_tag="ACHOUK_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9899387.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            complement(4921..5139)
                     /locus_tag="ACHOUK_004044"
     CDS             complement(4921..5139)
                     /locus_tag="ACHOUK_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFP9899388.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            complement(5179..5346)
                     /locus_tag="ACHOUK_004045"
     CDS             complement(5179..5346)
                     /locus_tag="ACHOUK_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899389.1"
                     /translation="MHFRVTGEWNGEPFNRVIEAENINDCYDHWMIWAQIAHADITNI
                     RIEELKEHQAA"
     gene            5589..6191
                     /locus_tag="ACHOUK_004046"
     CDS             5589..6191
                     /locus_tag="ACHOUK_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899390.1"
                     /translation="MSYFLRKKWMVNLSGAGKILWALNMKKDSYPYLICMTVLGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGMCIFAGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
     gene            complement(6402..6623)
                     /locus_tag="ACHOUK_004047"
     CDS             complement(6402..6623)
                     /locus_tag="ACHOUK_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFP9899391.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNHQAISATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            complement(6722..6937)
                     /locus_tag="ACHOUK_004048"
     CDS             complement(6722..6937)
                     /locus_tag="ACHOUK_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFP9899392.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQPFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            complement(7014..7205)
                     /locus_tag="ACHOUK_004049"
     CDS             complement(7014..7205)
                     /locus_tag="ACHOUK_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFP9899393.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMVAKAEADERDLV"
     gene            complement(7178..7360)
                     /locus_tag="ACHOUK_004050"
     CDS             complement(7178..7360)
                     /locus_tag="ACHOUK_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFP9899394.1"
                     /translation="MKHPHDNIRVGTITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKREAICTKHLLLS"
     gene            complement(7357..8037)
                     /locus_tag="ACHOUK_004051"
     CDS             complement(7357..8037)
                     /locus_tag="ACHOUK_004051"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFP9899395.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMAGFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            complement(8034..8705)
                     /gene="bet"
                     /locus_tag="ACHOUK_004052"
                     /pseudo
     CDS             complement(8034..8705)
                     /gene="bet"
                     /locus_tag="ACHOUK_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
     gene            8735..8917
                     /locus_tag="ACHOUK_004053"
     CDS             8735..8917
                     /locus_tag="ACHOUK_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFP9899396.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHCARKTR"
     gene            8914..9084
                     /locus_tag="ACHOUK_004054"
     CDS             8914..9084
                     /locus_tag="ACHOUK_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFP9899397.1"
                     /translation="MIDQNRSYEQESVERALTCANCGQKLHVLEVHVCEHCCAELMSD
                     SNSSMHEEKDDG"
     gene            9077..>9604
                     /locus_tag="ACHOUK_004055"
     CDS             9077..>9604
                     /locus_tag="ACHOUK_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFP9899398.1"
                     /translation="MAKPARRRCKNDECREWFHPAFANQWWCSPECGTKIALERRSKE
                     REKAEKAEKAAEKKRRREEQKQKDKLKIQKLALKPRSYWIKQAQQAVHAFIRERDRDL
                     PCISCGTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELIS
                     RIGQEAVEEIESNHNR"
BASE COUNT         2369 a         2326 c         2345 g         2564 t
ORIGIN      
        1 catttatttt cttgctgcgc acttcacggt gcgcagatat ctggagcatt tgatgatcaa
       61 gttatctgaa aaaggcgtgt ttctcgccag taataacgaa ataattgccg aagaacattt
      121 caccggcgaa attaaaaaag aagaagcaaa aaaaggcact attgcctggt ctattctctc
      181 ttctcataat acgtccggaa atatggataa acttaaaatt aagtttgatt cattagcctc
      241 tcacgatatt acctttgttg gtattgtaca gaccgctaaa gcgtccggta tggaacgttt
      301 cccgctgccg tatgtgctga ccaactgcca taactcactc tgcgccgtcg gcggcaccat
      361 taacggtgat gaccatgttt ttggtttatc ggcagctcag cgttatggcg gtatttttgt
      421 gcctccgcat attgcggtca tccatcaata tatgcgtgag atgatggcag gtggcggcaa
      481 aatgatcctc gggtcagaca gtcacacccg ttacggtgca ttagggacaa tggcagtcgg
      541 tgagggcggc ggcgagttgg taaaacaatt gcttaatgac acctgggata tcgactatcc
      601 gggagttgtt gcggtgcatc tgaccggaaa accagcgccg tatgtggggc cgcaggatgt
      661 ggcgttggct atcatcggtg ccgtgttcaa aaacggctac gtcaaaaaca aagtgatgga
      721 attcgtaggt cccggtgttg ctgcgctctc taccgatttc cgtaacagcg ttgacgttat
      781 gaccactgaa acgacctgtt taagttctgt ctggcaaacc gatgaagaag tccataactg
      841 gctggcgctg cacggtcgcg gccaggatta ctgccagctt aaccctcaac cgatggcgta
      901 ctacgatggc tgcatcagcg ttgatttaag cgccatcaaa ccaatgattg cgctgccgtt
      961 ccacccgagc aacgtgtatg aaatcgacac actgaaccag aacttgaccg acattctgcg
     1021 tgagattgaa attgagtccg aacgcgtggc gcacggtaaa gccaaactct cgctgctgga
     1081 caaagtggaa aatggtcgcc tgaaagtgca gcaggggatt atcgcgggct gttctggcgg
     1141 taactacgaa aacgtcatcg cggcggcgaa tgcactgcgc ggtcaatcct gtggcaatga
     1201 caccttctcg ctggcggttt acccgtcatc acagccggtg tttatggatc tcgccaaaaa
     1261 aggtgtggta gcagatttga ttggcgcagg cgcaatcatc agaaccgcgt tctgcggccc
     1321 atgctttggc gcgggcgata cgccaatcaa caacggtttg agtattcgcc acaccacgcg
     1381 taacttcccg aaccgcgaag gctctaagcc agctaatggg cagatgtcag cggtggcgtt
     1441 gatggacgct cgttctatcg ctgcgactgc ggcaaacggt ggctatttaa cctctgccag
     1501 cgaactcgat tgctgggaca acgtgccgga gtacgccttc gatgtaacgc cgtataaaaa
     1561 tcgcgtctat caaggctttg tgaaaggggc ggcccagcaa ccgctgattt acggaccgaa
     1621 cattaaagac tggccggaat tgggtgcgct gactgacaat atcgtcctca aagtgtgctc
     1681 gaagatcctc gacgaagtga ccaccaccga cgaattgatt ccttccggtg aaacctcttc
     1741 ttatcgttca aatccgattg gtctggcgga gtttaccctg tcacgccgcg atccaggtta
     1801 tgttggcaga agtaaagcga ctgctgagct ggaaaatcag cgtctggcgg ggaatgtcag
     1861 cgagctgaca gaggtgtttg cgcgcattaa gcagattgct ggtcaggagc atattgatcc
     1921 gctgcaaact gaaattggca gcatggtcta tgcggtgaaa ccaggcgatg gttctgcgcg
     1981 tgaacaggcg gcgagttgtc agcgtgtgat tggcggtctg gcgaatattg ccgaggagta
     2041 cgcgactaaa cgctatcgtt ctaacgtcat caactggggg atgttaccgc tgcagatggt
     2101 ggaagcgcca acctttgaag tgggggatta catttacatc cctggcatta aagcggcgct
     2161 ggataatccg ggtacgacgt ttaaaggtta tgtgatccat gaagatgcgc cggtaacgga
     2221 aattacgctc tatatggaaa gtctgactgc tgaagagcgc gagattatca aggcgggtag
     2281 tttgattaac ttcaataaaa accgtcagat gtaaaaagcg ccatgtgaat gtaggtcgca
     2341 ttcggcactt attgtcggat gcgatgctgg cgcatcttat ccgacctaca aatcgcatcg
     2401 aaccgtaggc cggataaggc gtttacgccg catccggcaa atagttaata gctcttactt
     2461 cttcgcctct gcaaccactt tgctacccac gccgcggtta ttgtattccc acatgcggtt
     2521 gtagttagtg tcattcagat tgcgctgtat ttcgtcgcta tcgtcaacgc tgccggtgtt
     2581 acccgcaaac ggacgattag agatcaccgc atcgccccac ggtttagccg tgttaaaacc
     2641 ttcgttgatg gcgctatcac ggatcaccac ctgaccgttg gtattggcat caacatccag
     2701 cgagcggccc agttgtgcca cgccatcacc ggaagcattg aaacggctgt ttacggcgag
     2761 gaaaccgtag taaatgttgg acagcgtagc cggtgcaaac acatacgctt cttgctgagt
     2821 acgtgagttc accacgcgga attcggtgtt atcgaacacc actgcgccgc gaccagaaac
     2881 gatatccaca tccccttcaa tgtagctgtt ggtcaccagc gtacgcggct gacgattcgt
     2941 ttccagacgg ttctgcacac cgctgttggt gacaaagaag gtgttctgac gaccgagaat
     3001 gttaacgttg ttaatctgta cctggtcacc gtcagtacgc agtgccaccg ccggatggtt
     3061 acccgcatct acgctatcac ccagcgtgtt ttcgatggtc aggttttgca gttgcaggcc
     3121 attgttttgt gaccagaaga ccgcagaaca cagaacaccg atactgtcgc tgcgtttgct
     3181 ctggcagcta tcgtacatat accacgctgg tttacctggc atatatttgc cgcgcgggtt
     3241 gacgtcgtga cgccagtcgg cagggctcat gcccccatca agggaaagcc caatcttcac
     3301 atcaatcggt ttttcacctg taccgtacag agtaattcca cctggagcag cagggacata
     3361 caccgttccc tgatactcac caggcatcac ggcaatatac tggcgcttgt tggtgcgctt
     3421 gataattgcc gcatctaccg ccgcctgaat cgtggtatgc gttacacctt gagtacccgc
     3481 cgggccgaca acaaagtcag gttgcgcagg cagggttatc ggggacggat tccacgctgc
     3541 agcacctggt gtcagggatg caaaatagtg ttgagcatcg aaattctgcg cttcttttgc
     3601 cgacagaatc gggcgagaag aggtaccagg cgcagtttga tcagaaggac gttgatcggg
     3661 tggtgttgag ctacaggcgg tcagcgtcac gccaaaagcc aatgccagcg ccagacggga
     3721 aactgaaaat gtgttcacag gttgctccgg gctatgaaat agaaaaatga atccgttgaa
     3781 gtctgctttt ttatactaag ttggcattat aaaaaagcat tgcttatcaa tttgttgcaa
     3841 cgaacaggtc actatcagtc aaaataaaat cattatttga tttcaatttt gtcccactcc
     3901 ctgcctctgt catcacgata ctgtgatgcc atggtgtccg acttatgccc gagaagatgt
     3961 tgagcaaact tatcgcttat ctgcttctca tagagtcttg cagacaaact gcgcaactcg
     4021 tgaaaggtag gcggatcccc ttcgaaggaa agacctgatg cttttcgtgc gcgcataaaa
     4081 taccttgata ctgtgccgga tgaaagcggt tcacgacgag tagatgcaat tatggtttct
     4141 ccgccaagaa tctctttgca tttatcaagt gtttccttca ttgatatccc gagagcatca
     4201 acatgcaatg ttgtagggat ggcaattttt acgcctgttt tgctttgttc gacataaaga
     4261 tatccatcta cgatatcaga ccacttcatt tcgcataaat caccaactcg ctgcccggta
     4321 acaacagcca gttccattgc aagtctgagc caacatggtg atgattctgc tgcttgataa
     4381 attttcaggt attcgtcagc cgtaagtctt gatctcctta cctctgattt tgctgcgcga
     4441 gtggcagcga ccgggtttgt tgttatatgg ccttcagcta ttgcctctcg gaatgcatcg
     4501 ctcagtgttg atctgattaa cttggctgac gccgccttgc cctcgtctat gtatccattg
     4561 agcattgccg caatttcttt tgtggtgatg tcttcaagtg gagcatcagg cagccccctc
     4621 cttattgctt taattttgct catgtaattt atgagtgtct tctgcttgat tcctctgctg
     4681 gcgaggattt tttcgtagcg atcaagccat gaatgtaacg taacagaatt atcactgttg
     4741 attctcgctg tcagaggctt gtgtttgtgt cctgaaaata actcaatatt ggcctgtatt
     4801 gcttcagtga ttgctatcct cctgtctcgg cctaaaccaa actctttacc cgtccttggg
     4861 tccctgtagc agtaatatcc attgtttctt atataaaggt tagggggtaa atcccggcgc
     4921 tcatgacttc gccttcttcc catttctgat cctcttcaaa aggctacctg ttactggtcg
     4981 atttaagtca acctttaccg ctgattcgtg gaacagatac tctcttccat ccttaaccgg
     5041 aggagggaat atcctgcatt cgcgcaccca tcgacgaact gtttcaaggc ttcttgggcg
     5101 tcgctggcgt gcgttccact cctgaagtgt caagtacatc gcaaagtctc cgcaattaca
     5161 cgcaagaaaa agccgccatc aggcggcttg gtgttctttc agttcttcaa ttcgaatatt
     5221 ggttatgtct gcatgtgcta tctgcgccca tatcatccag tggtcatagc agtcattgat
     5281 gttctctgct tcgataactc tgttgaatgg ttctccattc cattcacctg tgactcggaa
     5341 gtgcatttat catctccata aaacaaaacc cgccgtagcg agttcagata aaagaaatcc
     5401 tcgtcagtgc gaggatgctg ttcattgctg ctatacactt ttttgctctc aacgtaagcg
     5461 gtagctcatt ctgttgggtt ggtgcagttg cttttaggaa atgctattta ccccttaaac
     5521 gtcggctgaa agagctaaaa tccatgcaaa aaatttacgc aattttgtgt attattgtgc
     5581 agtaagtaat gagctatttt ctgcgcaaaa aatggatggt aaatttgtcc ggggcaggaa
     5641 aaattttatg ggcgctaaac atgaaaaaag attcgtatcc ttatttgatt tgcatgacag
     5701 ttttaggcct gatctttatt ttccttttct tctggtggcg ggcagatatt tacagggtca
     5761 cgtttcttaa tcagagtata tcccactatt acattctgtt tagcatggga atagcttttc
     5821 tgttatctct gttttgggtt aagaagggga tagtaaaaca aagcggctgg aagagtctgt
     5881 cagcatacct taaggtttat gcagggatgt gcatatttgc tggatttttt ctgattatac
     5941 cccttacaac actaacttat tttttgcctg gagagacatc gtcttatgtt gcaccgtatc
     6001 ggtatacttc cggtagttca aaaagttgtt ctggagctga ggtggatgac cccgatctac
     6061 atgagaatat tcgcatttgc tatccgtatg gcaattatga gtacgataat attatctatg
     6121 ttgaaaagaa aattaataca ttaggtgcgg tagtaacata tgcacagacc gcgcgtgatg
     6181 atactgaatg agatagtata tagcgggcaa gttttagtta atttatcgag gtaatataat
     6241 ttacctcgac tcgtttattc tggtattaat atttcgcttt acgatcgatt tttatctgat
     6301 gatatcatgc ggttttcata tactgactta ctgtcttttc tccgttagcg attttctcct
     6361 gctcagcgat gattttatct ttggcttcta gttaatttcg ctcacttcga acctctctgt
     6421 ttactgataa gttccagatc ctcctggcaa cttgcacaag tccgacaacc ctgaacggcc
     6481 aggcgtcttc gctcatctat gggatcgcca cactcacaac aatgagtggc agatatagcc
     6541 tggtggttca ggcggcgcat ttttattgct gtgttgcgct gtaattcttc aatttctgat
     6601 gctgaatcaa tgatgtctgc catcttccat taatccctga attgttggtt aatacgcttg
     6661 agggtgaatg cgaataataa aaaaggagcc tgtagctccc tgatgatttt gcttttcatg
     6721 ttcaccgttc cttaaagacg ccgtttaaca tgccgattgc cagacttaag tgagtcggtg
     6781 tgaatcccat cagcgttacc gtttcgcggt gcttcttcag tacgctacgg caaatgtcat
     6841 cgacgttttt atccggaaac ggctgtctgg cttttttgat ttcagaatta gcctgacggg
     6901 caatactgcg aagggcgttt tcttgctgag gtgtcattga acaagtccca tgtcggcaag
     6961 cataagcaca cagaatatga agcccgctgc cagaaaaatg cattcagtgg ttgtcatacc
     7021 aggtctctct catctgcttc tgctttcgcc accatcattt ccagcttttg cgaaagggat
     7081 gtggctaacg tatgaaattc ttcgtctgtt tctactggta ttggcacaaa cctgactcca
     7141 atttgagcaa ggctatgtgc catctcaata ctcgttctta actcaacagg agatgctttg
     7201 tgcatatcgc ctcccgttta ttatttatct cctcagccag ccgctgggct ttcagcggat
     7261 ttcggataac agaaaggccg ggaaataccc agcctcgctt cgtaacggag tagacgaaag
     7321 tgatcgtgcc tacgcggata ttatcgtgag gatgcttcat cgccattgct ccccaaatac
     7381 aaaaccaatt tcagccagtg cctcgtccat tttttcgatg aactccggca ccatctcgtc
     7441 aaaacccgcc atgtactttt catcccgctc aaccacgaca taatgcaggc cttcacgctt
     7501 catacgcggg tcatagttgg caaagtacca ggcatctttt cgcgtcaccc acatgctgta
     7561 ctgcacctgg gccatgtaag ccgactttat ggcctcgaaa ccaccgagcc tgaacttcat
     7621 gaaatcccgg gaggtaaacg ggcatttcag ctcaaggccg ttgccgtcac tgcataaacc
     7681 atcgggagag caggcggtgc gcatactttc gtcgcgatag atgatcgggg attcagtaac
     7741 attcacgccg gaagtgaatt caaacagggt tctggcgtcg ttctcgtact gttttcccca
     7801 ggccagcgcc ttagcattaa cttccggagc cacaccggtg caaacctcag ccagcagggt
     7861 gtggaagtag gacattttca tgtcaggcca cttctttcct gagcggggct ttgctatcac
     7921 gttgtgaact tctgaagcgg tgatgacgcc gagccgtaat ttgtgccatg catcatcccc
     7981 ctgttcgaca gctctcacgt cgatcccggt acgctgcagg ataatgtccg gtgtcatgct
     8041 gccaccttct gctcagtggc tttctgtttc aggaatccaa gagctttcac tgcttcggcc
     8101 tgtgtcagtt ctgacgatgc gcgaatgtcg cggcgaaata tctgggaaca gagcggcaat
     8161 aagtcgtcat cccatgtttt atccagggcg attagcagag tgttaatctc ctgcatggtt
     8221 tcatcgttaa ccggagtgat gtcgcgttcc ggctggcgtt ctgcagtgta tgcagtattt
     8281 tcgacaatgc gctcggcttc atccttgtca tagataccag caaatccgaa ggccagacgg
     8341 gcacactgaa tcatagcttt atgccgtaac atccgtttgg gatgcgactg ccacggcccc
     8401 gtgatttctc tgccttcgcg ggttttgaat ggttcgcggc ggcattcatc catccattcg
     8461 gtaacgcaga tcggatgatt acggtccttg cggtaaatcc ggcatgtaca ggattcattg
     8521 tcctgctcaa agtccatgcc atcaaactgc tggttttcat tgatgatgcg ggaccagcca
     8581 tcaacgccca ccaccggaac gatgccgttc tgcttatcag ggaaggcgta aatttctttc
     8641 gtccacggat taaggccgta ctggttggcg acgatcaaca atgcgatgaa ctgcgcatcg
     8701 ctggcatgaa agcgcgatgg ggagacaggg cggcatgaga cgacagcgac gaagtttcac
     8761 cgacatcatc tgcgaaaact gcaaatacct tccaacgaaa cgctccagaa ataaacgcaa
     8821 gccaatccca aaagaatctg acgtaaaaac cttcaactac acggctcacc tgtgggatat
     8881 ccggtggcta agacattgtg cgaggaaaac aaggtgattg accaaaatcg aagttacgaa
     8941 caagaaagcg tcgagcgggc tttaacgtgc gctaactgcg gtcagaagct gcatgtgctg
     9001 gaagttcacg tgtgtgagca ctgctgcgca gagctgatga gcgattcgaa tagctcgatg
     9061 cacgaggaaa aagatgatgg ctaaaccagc gcgaagacga tgtaaaaacg atgaatgtcg
     9121 ggaatggttt caccctgcat tcgctaatca gtggtggtgc tctccagagt gtggaaccaa
     9181 gatagcactc gaacgacgaa gcaaagaacg cgaaaaagcg gaaaaggcag aaaaggcagc
     9241 agagaagaaa cgacgacgag aggagcagaa acagaaagat aaacttaaga ttcaaaaact
     9301 cgccttaaag ccccgcagtt actggattaa acaagcccaa caagccgtac acgccttcat
     9361 cagagaaaga gaccgcgact taccatgtat ctcgtgcgga acgctcacgt ctgctcagtg
     9421 ggatgccgga cattaccgga caactgctgc ggcacctcaa ctccgatttg atgaacgcaa
     9481 tattcacaag caatgcgtgg tgtgcaacca gcacaaaagc ggaaatctcg ttccgtatcg
     9541 cgtcgaactg attagccgca tcgggcagga agcagtagag gaaatcgaat caaaccataa
     9601 ccgc
//
LOCUS       DAZKIP010000147         9589 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZKIP010000147 DAZKIP010000000
VERSION     DAZKIP010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.148"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..988
                     /gene="pgaA"
                     /locus_tag="ACHOUK_004056"
     CDS             <1..988
                     /gene="pgaA"
                     /locus_tag="ACHOUK_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:1901515 -
                     poly-beta-1,6-N-acetyl-D-glucosamine transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HFP9899399.1"
                     /translation="LTLQEWQQAAVLTHDVVEREPQDPGVVRLKRAVDVHNLAELRIA
                     GSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADGQFSEGKGIVRDWLAG
                     VEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGSQLERLSHRVPLRAMK
                     NGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLEGQERIWSSPYLIVDF
                     LPSLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSWEQIFSAGVGASWQKH
                     YGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLYVEFDMTFRF"
     gene            997..3015
                     /gene="pgaB"
                     /locus_tag="ACHOUK_004057"
     CDS             997..3015
                     /gene="pgaB"
                     /locus_tag="ACHOUK_004057"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HFP9899400.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRKSLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDELVD
                     REYFATWQQVREVARSRLVELASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKVEVNPHVFIWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKSPQRIMHIDLDYVYDENLQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIRQNPEQFKQWAR
                     FKSRALTDFTLELSARVKAIRGPHIKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYLEGVAEKSADQWLIQLTNQIKNIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDLIRPEFSTAWYPKND"
     gene            3008..4333
                     /gene="pgaC"
                     /locus_tag="ACHOUK_004058"
     CDS             3008..4333
                     /gene="pgaC"
                     /locus_tag="ACHOUK_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
                     /protein_id="HFP9899401.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERRWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYSLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            4335..4748
                     /gene="pgaD"
                     /locus_tag="ACHOUK_004059"
     CDS             4335..4748
                     /gene="pgaD"
                     /locus_tag="ACHOUK_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HFP9899402.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(4798..5586)
                     /gene="phoH"
                     /locus_tag="ACHOUK_004060"
     CDS             complement(4798..5586)
                     /gene="phoH"
                     /locus_tag="ACHOUK_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFP9899403.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(6206..7477)
                     /gene="efeB"
                     /locus_tag="ACHOUK_004061"
     CDS             complement(6206..7477)
                     /gene="efeB"
                     /locus_tag="ACHOUK_004061"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFP9899404.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPVARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKGIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(7483..8610)
                     /gene="efeO"
                     /locus_tag="ACHOUK_004062"
     CDS             complement(7483..8610)
                     /gene="efeO"
                     /locus_tag="ACHOUK_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFP9899405.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(8668..9498)
                     /gene="efeU"
                     /locus_tag="ACHOUK_004063"
     CDS             complement(8668..9498)
                     /gene="efeU"
                     /locus_tag="ACHOUK_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFP9899406.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
BASE COUNT         2468 a         2398 c         2277 g         2446 t
ORIGIN      
        1 attaacgtta caagaatggc agcaggcagc tgtcttaacg cacgatgttg tcgaacgtga
       61 accgcaagat cccggcgttg tacgattaaa acgtgcggtt gatgtacata atcttgcaga
      121 gcttcgtatc gctggctcaa caggaattga tgccgaaggc ccggatagtg gtaaacatga
      181 tgtcgactta accaccatcg tttattcacc accgctgaag gataactggc gcggttttgc
      241 tggattcggt tatgccgatg gacaatttag cgaaggaaaa gggattgttc gcgactggct
      301 tgcgggtgtt gagtggcggt cacgtaatat ctggctcgag gcagagtacg ctgaacgcgt
      361 tttcaatcat gagcataaac ccggcgcgcg cctgtctggc tggtatgatt ttaatgataa
      421 ctggcgtatt ggttcgcaac tggaacgcct ctctcaccgc gttccattac gggcaatgaa
      481 aaatggtgtt acaggcaaca gtgctcaggc ttatgttcgc tggtatcaaa atgagcggcg
      541 taagtacggt gtctcctggg ctttcactga tttttccgac agtaaccagc gtcatgaagt
      601 ctcacttgag ggtcaggaac gcatctggtc ttcaccatat ttgattgtcg atttcctacc
      661 cagtctgtat tacgaacaaa atacagaaca cgatacccca tactacaacc ctataaaaac
      721 gttcgatatt gttccggcat ttgaggcaag ccatttgtta tggcgaagct atgaaaatag
      781 ctgggagcaa atattcagcg caggtgttgg tgcctcctgg caaaaacatt atggcacgga
      841 tgtcgtcacc caactcggct acgggcaacg cattagctgg aatgacgtga ttgatgctgg
      901 cgcaacgcta cgctgggaaa aacgacctta tgacggtgac agagaacaca acttatacgt
      961 tgaattcgat atgacattca gattttaagg ataaatatgt tacgtaatgg aaataaatat
     1021 ctcctgatgc tggtgagtat aattatgctc accgcgtgca ttagccagtc aagaacatca
     1081 tttataccgc cacaggatcg caaatcttta ctcgccgagc aaccgtggcc gcataatggt
     1141 tttgtagcga tttcatggca taacgttgaa gacgaagctg ccgaccagcg ttttatgtca
     1201 gtgcggacat cagcactgcg tgaacaattt gcctggctgc gcgagaacgg ttatcaaccg
     1261 gtcagtattg ctcaaattcg tgaagcacat cgaggaggaa aaccgctacc ggaaaaagct
     1321 gtagtgctga cttttgatga cggctaccag agtttttata cccgcgtctt cccaattctt
     1381 caggccttcc agtggcctgc tgtatgggcc cccgtcggca gttgggtcga tacgccagcg
     1441 gataaacaag taaaatttgg cgatgagttg gtcgatcgag aatattttgc cacgtggcaa
     1501 caagtgcgag aagttgcgcg ttcccggctc gttgagctcg cttctcatac atggaattct
     1561 cactacggta ttcaggctaa tgccaccggc agcttattgc ctgtatatgt aaatcgtgca
     1621 tattttactg accacgcacg gtatgaaacc gcagcagaat accgggaaag aattcgtctg
     1681 gatgctgtaa aaatgacgga atacctgcgt acaaaggttg aggtaaatcc acacgttttt
     1741 atttggcctt atggcgaagc gaatggcata gcgatagagg aattaaaaaa actcggttat
     1801 gacatgttct tcacccttga atcaggtttg gcaaatgcgt cgcaattgga ttccattccg
     1861 cgggtattaa tcgccaataa tccctcatta aaagagtttg cccagcaaat tattaccgta
     1921 caggaaaaat caccacaacg gataatgcat atcgatcttg attacgttta tgacgaaaac
     1981 ctccagcaaa tggatcgcaa tattgatgtg ctaattcagc gggtgaaaga tatgcaaata
     2041 tcaaccgtgt atttgcaggc atttgctgat cccgatggtg atgggctggt caaagaggtc
     2101 tggtttccaa atcgtttgct accaatgaaa gcagatattt ttagtcgggt tgcctggcaa
     2161 ttacgtaccc gctcaggtgt aaacatctat gcgtggatgc cggtattaag ctgggattta
     2221 gatcccacat taacgcgagt aaaatactta ccaacagggg agaaaaaagc acaaattcat
     2281 cctgaacaat atcaccgtct ctctcctttc gatgacagag tcagagcaca agttggcatg
     2341 ttatatgaag atcttgccgg acatgctgct tttgatggca tattgttcca cgatgatgct
     2401 ttgctttcag attatgaaga tgccagtgca ccggctatca cggcttatca gcaagcaggc
     2461 tttagcggga gtctgagcga aattcgacaa aacccggagc aatttaaaca gtgggcccgc
     2521 tttaaaagtc gtgcgttaac tgacttcact ttagaactta gtgcgcgcgt aaaagccatt
     2581 cgcggtccac atattaaaac tgcacgaaat atttttgcac ttccggtaat acaacctgaa
     2641 agtgaagcct ggtttgcaca gaattatgct gatttcctaa aaagctatga ctggaccgct
     2701 attatggcta tgccttatct ggaaggtgtc gcagaaaaat cggctgacca atggttaata
     2761 caattgacca atcaaattaa aaacatccct caggctaaag acaaatctat tttagaatta
     2821 caggcacaaa actggcagaa aaatggtcag catcaggcta tttcttcgca acaactcgct
     2881 cactggatga gcctattaca actgaatgga gtgaaaaact atggttatta tcccgacaat
     2941 tttctgcata accaacctga aatagacctt attcgtcctg agttttcaac agcctggtat
     3001 ccgaaaaatg attaatcgca tcgtatcgtt ttttatatta tgtctggtgt tatgcatacc
     3061 cctatgcgta gcgtactttc actctggtga actgatgatg aggttcgttt tcttctggcc
     3121 gttttttatg tccattatgt ggattgttgg cggcgtctat ttctgggtct atcgtgaacg
     3181 ccgctggccg tggggagaaa acgcaccagc tccccagttg aaagataatc cgtctatctc
     3241 cattatcatt ccctgtttta atgaggagaa aaacgttgag gaaaccatac acgccgcttt
     3301 agcacagcgt tatgagaaca ttgaagttat tgccgtaaat gacggttcaa cagataaaac
     3361 ccgtgccatc ctggatcgca tggctgcaca aattccccat ttgcgggtca ttcatctggc
     3421 gcaaaaccag gggaaagcca ttgcgcttaa aaccggagct gccgcggcga aaagtgaata
     3481 tctggtgtgc attgatggcg atgcgttatt agaccgcgat gcggcggcat atattgtgga
     3541 accgatgttg tacaacccgc gtgtgggtgc cgtaaccggt aatcctcgta ttcgaacacg
     3601 ttctaccctg gtgggtaaaa ttcaggttgg cgagtattcc tcaattattg gtttgatcaa
     3661 gcgaacccag cgtatctatg gaaacgtatt taccgtttcc ggtgttattg ccgcatttcg
     3721 tcgcagcgcc ctggcagaag tgggttactg gagtgacgat atgatcaccg aagatattga
     3781 tattagctgg aagctgcagt tgaatcagtg gacgattttt tacgagccac gggcactgtg
     3841 ctggatatta atgcctgaaa cgttaaaagg gctgtggaaa cagcgcctgc gctgggctca
     3901 gggcggtgca gaagtattcc tcaaaaatat gacaaggttg tggcgcaaag aaaactttcg
     3961 aatgtggccg ctgttttttg aatacagcct gacgacaata tgggccttca cctgcctggt
     4021 cggtttcatt atttacgcag tccaacttgc cggtgtaccg ttaaatattg aattgacaca
     4081 tatcgctgcg acacatactg ccggaatatt attgtgtacg ttatgtttac tgcaatttat
     4141 tgtcagcctg atgatcgaga atcgctatga gcataatctg acttcatcgc ttttctggat
     4201 tatttggttc ccggttattt tctggatgct gagcctggca acgacattgg tatcatttac
     4261 acgagtcatg ttgatgccta aaaagcaacg cgcccgttgg gtaagtcccg atcgcgggat
     4321 tctgagaggt taatatgaac aatttaatta ttacgacccg acaatcacca gtacgtttac
     4381 tggttgatta tgttgccaca accatcttgt ggacattatt tgcgttgttc atattcttat
     4441 tcgccatgga tctgctgacg ggttattact ggcaaagcga ggccagaagc cgacttcagt
     4501 tctatttttt gctggcagtg gcgaatgccg tcgtgttaat tgtctgggcg ctgtacaata
     4561 agctgcgttt tcaaaaacag cagcatcatg cagcctacca atatacgccg caagaatatg
     4621 cagagagctt agcaatacct gatgagctct atcagcaact acaaaaaagc cacaggatga
     4681 gcgtacactt caccagccag gggcaaataa aaatggttgt ttcagaaaaa gcgctagtca
     4741 gggcataaac acccaaaaca aagcccggtt cgcccgggct ctgcaccgat aatacactta
     4801 actgtatgca tgcagcgtac gttggcaaag tgccgaacgt acgcaatcct cttttccgaa
     4861 ccggacgatc ccaaccattt catcttcttc gaaacgttcc agcgcgtcac ttaatccgga
     4921 gcacacgccg cgaggcaaat cgcattgcgt gatatcaccg ttgacgataa ccgtcacgtt
     4981 ctccccgagg cgggttaaaa acattttcat ttgcgcggca gtcacattct gcgcctcgtc
     5041 aagaatgacg actgcatttt caaaggtacg tccacgcata taggcgaacg gcgcaatttc
     5101 caccttccct atttccggtc gcaggcagta ctgcataaag gaagccccta agcgccggac
     5161 cagcacgtcg tagaccgggc gaaaataggg agcaaacttt tctgcgatat ctccaggtaa
     5221 gaagccaaga tcttcatcgg cttgcagaac tggacgggtg acgataatcc tgtcgacatc
     5281 cttatgtatc agggcctctg ccgcttttgc tgcgctgatc caggtttttc cgcacccggc
     5341 ttcgcccgtg gcgaatatca gctgcttact ctcaatagcc ttcagatagt gcaattgcgc
     5401 ttcatttcgc gcgaggatgg gcgaagtatc gcgactgtcg cgggccatac caatggcttc
     5461 tacgccgccc atctgcacaa gcgaggtgac cgattcttct tcacgctgct tatggctgcg
     5521 cgaatcccgt ctcagcacac gttttgcctc gcgacgagct ttgatcactg ctttttgtct
     5581 tcccatggag agcaccttga gttgtttgta ttcatcacac gcgccgttgg cagcgcgatt
     5641 atgcgcacga acatcagagg gttggcttcc ttgtaagcca tagtttgctt ttggataaaa
     5701 tgccgaaaaa cggctacgcg caccgtttac ggcgtcggta acacatgaaa agaaaggatg
     5761 aggttgaaaa tgcaaagtga cgagatgact accggaggag aaaactccgc gagtggtggc
     5821 gcgttgatta tctaaaacat gtccagtaca ggacgttacc atccgcgatc tccatagtga
     5881 ctgactatca ctgccgggaa cttccgctgc tacttaataa gtacaacagt tctcgcattt
     5941 attgcaacaa tatatttact tatatttaac tataaaacac catttcagtg acattagttt
     6001 ctactggaaa gatgacagag tgatgacaga tatgaaaaaa gctgtgtgct ttcagcagga
     6061 tttgcatcag gcatcagtgc acgatcgccg tatgcggcgt gaacgcctta tccgcctacc
     6121 gaacccgcac cgattgtagg cataagaaga cgcgcaagca tcgcatcagg catctgcgca
     6181 agaacgcctt atccgtctaa aaacattaaa cccgcaataa cgcgcttccg agataatcgt
     6241 tcgcgtcctt cactcccggc agcgcaaaaa aataaccgcc gccgataggt ttaacgtatt
     6301 cctccagcgc ttcgccattg agcctttttt gtactgtcag gaagcctttt tccagatcgt
     6361 gttggtaaca gacaaacagt agccccatat ccagttgccc ggagttggtg acgcccagtg
     6421 aataactgta gccacgacgc agcatcaggc tggactcact ctccgccgtg cggggattcg
     6481 ccagccggat atggctgtcc agcgcaatcc ccttcccttc cgggtcgctg gcgtaatcag
     6541 gcacatcatg ctcatgctgc attcccagcg gcgcaccggt ttgcttatca cggccaaaaa
     6601 tcgtctgctg ttctttcagc ggcgttctgt cccaaaattc cactcgaaac tgaatcaagc
     6661 gtactgcctg atagctgcca ccgattgtcc acgcaggctc ctgctgatct gccgttaccc
     6721 acacaacttt ttgcatcaac ttatcattct ggctatcggg attggcagtg ccgtctttga
     6781 aacccagcaa attaatcggc gtctctttgc ctttactacg cgccgcgtga tcggaaataa
     6841 acccttcccg cttccagcgc acgctgagca aatccggcgt atgtttgatg atatcgcgca
     6901 gtgcatggat caccgtgtcc tgggtgttgg cgcaaatttg tagcaacaca tcaccatgac
     6961 ataacgccgc atccagcgag tcgttgggaa aacgcgtcat cttctgcaac tttttcggca
     7021 tctgtggcgc aaggccaaag cgctcatcaa acaatgagtg acccaccgat aacgtgatgg
     7081 tgagattatc gggcgcaatg tagccgccaa gaatgccgga atcgagtggt ggcaggcgcg
     7141 gatttggcgt ttctggtgcc gctccgccct gagtcagaaa agcaaaacgc tgagtcaaca
     7201 agcgaaacaa ccgttcaaga tcggctttat cgctggcaag cacatcaaac gccaccagca
     7261 tcattgcggc ctgttgtggc gtcaggatcc ctgcctgatg ctcaccataa aacggctgtt
     7321 tctcattgcg tgcaaccggt gaaagcgtac ccggcgcact ttgcgttttt tgtgcatgag
     7381 cgaccggaca acttcccgcc agcgccagcg caccaatgcc tttcagtaaa cgtcggcgtg
     7441 acggttcatt cacgccgttt ttatcttcat actgcataat gcttaatcca atcccagcac
     7501 accgcgaagt tgcgccagat cttccgccag cgcagtaatc ggtcctttca gtgcattccg
     7561 gtcggcatcg gtcaatttgt cgtaggtttc aaaaccgtct ttagtacggt atttcgccag
     7621 aatggtatcg acctttttaa agttggcatc gacttttgcc aacagttccg ggttagcttt
     7681 ttgcagttgt ggacgcagca gatcgacaat tttctgcgag ccttcaacgt tagcctggaa
     7741 atcccacaga tcggtgtggc tgtagcgatc ttcttcaccg ctgattttgc tggctgccac
     7801 ttcctcaatc agtccggctg caccgccgac cacttttgaa ggtgggaaag ccagttcact
     7861 gatgcgtttt tgcaaatcga ccacatcggt ataaagctgg tcagcgtact gatccatgcc
     7921 tttggtggtg ttgtcgccaa acaatgcttt ttccagacgg tggaaaccgg tgaattttgg
     7981 atcagcggct ttttgctcgt aatcatcttc acgggcgtca atgctgccat ccagatcgga
     8041 gaacagttca gcaatcggtt caatgcgctc atagtgctgg cgcgtcggcg catacagtgc
     8101 tttcgctttt tcgatatcgc ctgctttaat cgcgtcggta aaggctttgg tgtcggtcac
     8161 cagctgtgtg gtttccgcca tgacatacgc tttatatgca gtaattgcac caccaagact
     8221 taacagcgca tcactttgcg ccgcatccgc cgttgcctca cctttgacga tcaacttccc
     8281 tttcgggtta gtcagcagac cgcaggtcat atcgtattcg ccaggctgta aattcgccgt
     8341 cattttctgg ctaaagccag gggcgatatt ttcccgctct tccaccacca tcacgccttt
     8401 gaggatctcc cattccagcg ccttctggct gtggttctga ataatgaact gtgttttccc
     8461 ggcgttaacc gtaatggtca tcggttcgca ctgcttatcc gtcacggtca ctttgacctg
     8521 cggcacatca gcggcgttag ccataaaagc agaagaaaac agcgcagcca cgctcaactg
     8581 caatgcgtta cggcggaagt taatggtcat gacgagacta tccctttaaa gtatgttgta
     8641 actaagcaag agtttgcgtc gtatttgtta cgcggagcga gacgctgtcg cccctgcgcg
     8701 tggtggcaga gcaaatgcca ccagcgccgg gatgagataa ataaaccaga cggcgacttc
     8761 gctgacgctc ggcgcttcct gatagccaaa aatcccttcc atcagcgtgc caaacagcga
     8821 gtgagttgag agcaccgcac tcatatcgaa ggcgatttcc tgaaagtggt tccacaatcc
     8881 ggcttcatga aatgcgcgaa tggcaccagc tgccagccct gcggcgacga agagaataaa
     8941 caggctggtc catttaaaaa atgcaccaag attgaggcga ataccgcccc agtagagcag
     9001 gaagcctagc accacggcag tagcaagacc gagcattgca cccagcggcg gccagatccc
     9061 gacatcttgt tgaaatgccg ccagcaggaa aaagaccgac tccagccctt cccttgcaac
     9121 ggcaaaaaag accatcatca ccagcgccca gccatgatga tttccacgct gcaatgcgct
     9181 atcgacagcc tgctccagtt gtactttgac gttgcgcgac actttgcgca tccagaaaac
     9241 catccaggta aggatcacca cggcgatcac cgccacgata ccttcaaaca gttcctgctc
     9301 tttttgcgga aattcgccgg tggtttcgtt aatgaagatc cccaagccca ggcacaacgc
     9361 agcggcaagc aacacgccaa tccacatcac accaatccat cggcctcgct gggtacgctt
     9421 aagatagctg gcaatcaaac tgacaatcag cgcggcttca agtccttcgc gcaacataat
     9481 gagaaacgga acaaacatgc cacccaccct taaacgtttt tctgagtcaa tctatgcaaa
     9541 gaaaagtaaa tcgcagtgat agcgattatc attacgctaa agaaaaata
//
LOCUS       DAZKIP010000148         9476 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZKIP010000148 DAZKIP010000000
VERSION     DAZKIP010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.149"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(120..2111)
                     /gene="tkt"
                     /locus_tag="ACHOUK_004064"
     CDS             complement(120..2111)
                     /gene="tkt"
                     /locus_tag="ACHOUK_004064"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFP9899407.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            2389..3147
                     /gene="loiP"
                     /locus_tag="ACHOUK_004065"
                     /gene_synonym="yggG"
     CDS             2389..3147
                     /gene="loiP"
                     /locus_tag="ACHOUK_004065"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFP9899408.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(3353..4273)
                     /gene="speB"
                     /locus_tag="ACHOUK_004066"
     CDS             complement(3353..4273)
                     /gene="speB"
                     /locus_tag="ACHOUK_004066"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFP9899409.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(4409..5140)
                     /locus_tag="ACHOUK_004067"
     CDS             complement(4409..5140)
                     /locus_tag="ACHOUK_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP9899410.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     TVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGQAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            complement(5286..7262)
                     /gene="speA"
                     /locus_tag="ACHOUK_004068"
     CDS             complement(5286..7262)
                     /gene="speA"
                     /locus_tag="ACHOUK_004068"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFP9899411.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(7271..7402)
                     /gene="yqgB"
                     /locus_tag="ACHOUK_004069"
     CDS             complement(7271..7402)
                     /gene="yqgB"
                     /locus_tag="ACHOUK_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFP9899412.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(7496..7639)
                     /gene="yqgG"
                     /locus_tag="ACHOUK_004070"
     CDS             complement(7496..7639)
                     /gene="yqgG"
                     /locus_tag="ACHOUK_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFP9899413.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(7750..8001)
                     /gene="yqgD"
                     /locus_tag="ACHOUK_004071"
     CDS             complement(7750..8001)
                     /gene="yqgD"
                     /locus_tag="ACHOUK_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFP9899414.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            8057..9211
                     /gene="metK"
                     /locus_tag="ACHOUK_004072"
     CDS             8057..9211
                     /gene="metK"
                     /locus_tag="ACHOUK_004072"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFP9899415.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         2347 a         2541 c         2392 g         2196 t
ORIGIN      
        1 atttattgca tataaatatt tgcgatctac aacacgcttt atctattgct tgtccgcaaa
       61 cggacatatc aaggtaataa aaaaggtcgc cgaagcgacc tttttacccg aaatgctagt
      121 tacagcagtt cttttgcttt cgcaacgacg ttgtcgacgg tgaagccgaa ctcttcgaac
      181 agcaactctg ccggagctga ctcaccgaag gtggtcatac cgatgatagc gccattcagg
      241 ccaacatact tgtaccagta gtcagcaata cccgcttcta ccgcaacgcg tgcagtaacc
      301 gctttcggca gtacggattc acggtaagcc gcatcctgct tgtcgaatgc atcggtagac
      361 ggcatggaaa ccacgcgcgc cttcacgcct tcggcagtca gtttttcgta ggcggcaaca
      421 gccagttcaa cttccgaacc ggtcgcgatg aagatcagtt ccggctgacc agcacagtct
      481 ttcagcacat aaccaccgcg cgcgatgttt gccagttgct cttcagtacg ttcctgctgc
      541 gccaggttct gacgggagag gataagcgcg gtcggaccgt cctgacgctc aacaccgtat
      601 ttccacgcga ccgcggattc aacctggtca cacggacgcc atgtagacat gttcggggtc
      661 acgcgcagag aagcaacctg ctcaaccggc tggtgagtcg ggccatcttc gcccagaccg
      721 atggagtcgt gggtgtaaac cataacctga cgctgtttca tcagcgcagc catacgtacg
      781 gcgttacgtg cgtattcgac aaacatcagg aaggtggagg tgtacggcag gaagccaccg
      841 tgcagggaga taccgttagc aatcgcggtc ataccgaact cgcgaacacc gtagtggatg
      901 tagttacccg cagcatcttc gttgattgct ttagaaccag accacagggt caggttagac
      961 ggtgccaggt cagcggagcc gccgaggaat tccggcaaca gcgggccgaa cgcttcgata
     1021 gcattctgag acgctttacg gctggcgatt ttcgccggat tagcctgcag tttagcgata
     1081 aactctttcg ctttggcgtc gaagtcagac ggcatttcgc ctttcatacg gcgggtaaat
     1141 tcagccgctt cctgcggata agctttcgcg taagccgcaa acttctcatt ccatgcagat
     1201 tctttcgcct ggcctgcttc tttcgcatcc cactgcgcat agatttcaga cgggatttcg
     1261 aacggtgcgt atttccagcc cagctgttcg cgggtcagag cgatttctgc atcacccagc
     1321 ggcgcaccgt gggagtcgtg agtaccggct ttgttcgggg aaccgaaacc gatgatggtt
     1381 ttgcacatca gcagggacgg tttgtcagtc actgcgcgcg cttcttctac tgcgcgtttg
     1441 atggatgctg cgtcatgacc gtcgatgtcg cgaataacgt gccagccgta agcttcgaaa
     1501 cgcattgcgg tgtcgtcggt gaaccagcct tcaacgtgac catcgataga aataccgttg
     1561 tcatcgtaga acgcaatcag tttacccagc ttcagcgtac ccgccagaga gcaaacttcg
     1621 tgggagatgc cttccatcat gcagccgtcg cccatgaagg cgtaggtgta gtggtcgaca
     1681 atgtcgtggc ccggacggtt aaactgcgcc gccagcgttt tttctgcaat cgccatacct
     1741 actgcgttgg caataccctg ccccagcgga ccggtggtgg tttccacacc agcggtgtaa
     1801 cccacttccg ggtggcccgg agttttagag tgcagctgac ggaagttttt cagttcttcc
     1861 atcggcagat cgtaaccggt gaggtgcagc aggctgtaga tcagcatgga gccgtggccg
     1921 ttggacagca cgaagcggtc acggtcagcc caggacggat tctgcgggtt atgtttcagg
     1981 aaatcacgcc acaggacttc ggcaatgtca gccataccca taggggcacc cgggtgaccg
     2041 gatttggctt tctgtactgc gtccatgctc agcgcacgaa tagcattggc aagctcttta
     2101 cgtgaggaca ttttgactcc agatcggatg atgaagggca cgcccttaac gacttgacga
     2161 cagcgcgttt tgggctacgc cggaaaattt gccaacaatt taccgcaagc cgcgcgtcat
     2221 gtacatggaa catccttttg ccgcttcaga aatctctgga tcatgctcgc atgttgcgca
     2281 atctactcgc ccgtccgctg cgcttttcct tatactgaga ctgagcgtcg gttcacctgc
     2341 aaacggcgca tttttagaat aatcctgacc ttgtgcggaa gaaaaaacat gaaaattcgc
     2401 gccttattgg tagcaatgag cgtggcaacg gtactgaccg gttgccagaa tatggactcc
     2461 aacggactgc tctcatcagg agcggaagct tttcaggctt acagtttgag tgatgcgcag
     2521 gtgaaaaccc tgagcgatca ggcatgtcag gagatggaca gcaaggcgac gattgcgcca
     2581 gccaatagcg aatacgctaa acgtctgaca actattgcca atgcgctagg caacaatatc
     2641 aacggtcagc cggtaaatta caaagtgtat atggcgaagg atgtgaacgc ctttgcaatg
     2701 gctaacggct gtatccgtgt ctatagcggg ctgatggata tgatgacgga taacgaagtc
     2761 gaagcggtga tcggtcacga aatggggcac gtggcattag gccatgtgaa aaaaggaatg
     2821 caggtggcac ttggtacaaa tgccgtacga gtagctgcgg cctcggcggg cgggattgtc
     2881 ggcagtttat ctcaatcaca actcggtgat ctgggcgaga aattagttaa ttcgcaattc
     2941 tcccagcgcc aggaagcaga agccgatgat tattcttacg atcttctgcg ccaacgcggc
     3001 atcagcccgg caggtcttgc caccagcttt gaaaaactgg caaaactgga agaaggtcgc
     3061 caaagctcaa tgtttgacga ccatcctgca tccgccgaac gcgcccagca tattcgcgat
     3121 cgcatgagcg cggatgggat taagtaaagc ctggtggtgt cgaacgtagg tcagataagg
     3181 cgttcacgcc gcatctgaca tgaatggcac atttgttacc ttgtgcgcat tgccggatgc
     3241 gatgctggcg catcttatcc ggcctacggg tgccgaacgt aggtcggata aggcgttcac
     3301 gccgcatccg acattaatgg cacgttttac ccgtgcgcat cgcatctggt gcttactcgc
     3361 cctttttcgc cgcctgaata tacagcattt ccagcgccag cgttgccgct gccagagcgg
     3421 tgatttccga ctgatcgtat gccggagcca cttccactac gtccatccca acgatgttga
     3481 gatctttcag gccgcgtacc agtttaatag cgcgatcgga ggtcagaccg ccaattaccg
     3541 gcgtaccggt tcccggcgca aatgcaggat ccaggcagtc gatatcaaag gtcaggtaaa
     3601 ccggcatatc acccacaatt tgtttcacct gggcgataac gtcatccacg ctgcgatcgt
     3661 tcacctggca ggcgtccagc acggtaaagc cgttgtcttt atcaaattcg gtacgaatac
     3721 caatctgcac ggaatgattc gggtcgatca gaccttcttt cggcgcggta tagaacatgg
     3781 tgccgtggtc aaattcacaa ccgttcgcgt aggtatcggt gtgggcgtca aagtgtacca
     3841 gcgccatttt gccgaaatgc ttcgcatgag cacgcagcag cggcagcgta acaaagtggt
     3901 caccaccgaa ggagagcata cgcttaccgg cagccagcag cttctcggcg tgcgcctgca
     3961 gcttctcgct catctcacgg gcatcgccga aagcatatac cagatcacca cagtccacga
     4021 cgttcagacg ctcacgcatg tcgaaattcc acgggaagcg gttgtgttcc caggccagat
     4081 tcgtcgaaac ctgacggata gctgccggac cgtggcgtcc ccccgcacga ccagaagtgg
     4141 ccatatcgaa cggcacacca gtaatcaccc agtcagcatc gctgtcatac ggctggaagt
     4201 tcatcggcag gcgcaaaaaa ccaaaggcgt tggaaaccag tgagttatcg tattgatgac
     4261 ctaaggtgct catggatgga ctcctattac aaagtcgata taaaaaaacc cttccgcgtc
     4321 gttaggcccg acgaggaagg gttggatttg cgaaacacaa gtttgtgcta attatcgcct
     4381 taaatgggcg aggcatcaag cgaggttgct atttccgaca cggtggcggc gcatagccat
     4441 tgttatctgc cggaaaggct tgtccagagc acggtggcag cgcgttttgc gcctggcctt
     4501 gtaattcaaa ggcggcaaag ccgattacac cggtattacg cacatcgcct tgcgccgaat
     4561 tggctgcgta ggaatcagca acgttggcga actgaaaagc cgcttcggta tgtttatctt
     4621 tgcggaatcc tttaatcgct aacgaatctc cggcattcac aatatagccg ttgttattca
     4681 gcgaaccttg cttgccgttc agcacatcca ggccatcaac cgtagcaacg atctcgtagt
     4741 tggtgttacg actgtaatta cggacatata attgataatt cattccgact ctggcatgga
     4801 caatatagcg tggttcatta ttagatgaat cataaacacg cgtaataggt agagaattaa
     4861 aattaccatc gcgaatagca tattccagtt cgttaatacg aatgctataa acgcgatcgt
     4921 agccggaagg aacattagtc gaataactaa taacgatcat ctccgccgga tcgcgccagg
     4981 ctcgctcagc agatacgccc tgaaccgttg agtgcacatc cccaccccag gcggtgccga
     5041 cattgcccga tgcactgcta ttatactgcg cattactgga acaaccggcc agcagcacga
     5101 ttaatgccac caacgcgctg cgtactttcc attttttcat aaccgttccc tctgcattta
     5161 attaatgcag cgattgtagg gagattattt aataagaaaa gcagctaaat tatttttaag
     5221 aatatataaa aagaaagcgg accgatttac gtaaataaat cgacccgcca gcagaataat
     5281 cttaattatt catcttcaag ataggtataa ccgtacaaac ctgcctcgaa ctcttcaagg
     5341 aactgttgtt gcagttcagc atcaagatcg gttttcttca cttgatcacg gaactgagtt
     5401 aacagcgttt tcggatcgag ctgtacatat tgcagcatgt ccgccacggt atcgccttcg
     5461 tcagacagtt ccacttctac actaccgtca gggaagacga acacgtcaac cgcttcggta
     5521 tcaccgaaca ggttgtgcat gttgccgagg atctcctgat atgcgccgac cataaagaaa
     5581 ccgagcatcg gcggattctc tggatcgtac tccggcatag gcattgtcgt ggcaatacca
     5641 tcaccatcga tatagtggtc gatagcaccg tcagagtcac aggtaatatc cagcagcaca
     5701 gcgcggcgtt ccggcacttg atccagccct tccagcggca gaaccgggaa caactggtcg
     5761 atcccccacg catccggcat cgactggaac agcgagaagt tgacgtacat tttgtccgcc
     5821 atacgttcct gcagctcgtc gataatcgga cgatgagcac ggttttgcgg atccagctgc
     5881 ttttgcactt catggcacat gctcaaataa agctgctcag cccatgcacg ttcttgcagg
     5941 ctaaaggtgc cggaagagta gccgatatga atgtcgtgca gatccatctg actgtcgtgt
     6001 aaccattcac gcagagaacg gcgagttccc ggttcgtgca tctcctgcca ggtttcccac
     6061 atgctttgca gcgcgcgcgg cgcatcttct gcaggcgcgg tcggcaccgt gtattcgtta
     6121 cgttccacgc cgatgatatt agacaccagc acggtgtgat gcgcagtcac tgcacgaccc
     6181 gattcggtga ttaccgtcgg atgcggcaga ccgttttctt cacacgcatc accaatcgcc
     6241 cagatgatgt tgttggcata ttcattgagg ccgtagttca ccgaacagtc ggactgcgaa
     6301 cgagtacctt cataatccac gcccagaccg ccgccgacgt cgaagcactg aatattgacg
     6361 cccagcttgt gcagctcaac atagaaacgc gcggattcac gaacgcctgt cgcgatatcg
     6421 cgaatattcg ccatctgcga accgaggtgg aagtgcagta gttgcaggct gtcgagacgc
     6481 ccggcttcac gtagggtttc aaccagttgc agtacctgag tcgccgccag gccgaacttc
     6541 gatttttccc cgccggagga ctgccattta ccggagccct gcgaagccag acgtgcacgc
     6601 acgcccagac gaggaacgac attcagacgt tctgcttcat ccagcacaat ggcgatttct
     6661 gacatcttct caatgaccag atagaccttg tgccccatct tctcgccaat taatgccagg
     6721 cggatatatt cgcggtcttt ataaccattg cagacgatga cgctacgggt catgccagca
     6781 tgtgccagca ctgccatcaa ctcggctttg gaaccggctt ccagacccag cggttcgccc
     6841 gaatgaatca gggactcaat cacgcgacgg tgctggttaa ctttgatcgg ataaacaagg
     6901 aagtaatcgc cgttatagcc gtaggattcc cgcgcacgtt tgaacgcggc gttaatggaa
     6961 cgcaaacggt gctgcaggat ctgtgggaaa cagaacagtg caggcagacg ctgaccctgt
     7021 gcttcacgag ttttcactaa ctgcgcgaga tcgacgcgag cttccgggac gtccgggtcc
     7081 gggcacacgc tgatgtggcc cagctcgtta acgtcatagt agttattgcc ccaccaggca
     7141 atattgtaag tacgcagcat cttgctggct tcctgggagc tcattgcaac ctcctgcatg
     7201 gagcgtagta caccgtgttc gcccgctgac gaaggcaaac ccatagacat gtcgtcagac
     7261 atagcgaacc tcaaattatt ttattaagtg taaaacagtt aacgactatc gcagcctgga
     7321 actgcgataa caacccataa gcacatggat tttccagcag tgaatgctga cgctccaact
     7381 gcgcaaccgg tttctttttc ataacattat taagcacata accgaacgta agtgtgacag
     7441 ttcggcgaaa ccacgagaaa actcttgttt ttacaagagc gcccttgttc agtcctcagt
     7501 aactgtaacc agctcttgaa tcctgagaag cgccgagatg ggtataacat cggcaggtat
     7561 gcaaagcaga gatgcagagt gcggggaacg aatcttcacc agaacggtga gacaggttaa
     7621 gcagcagaca acggttcatt atttcgtatc acctccacgg ccgcctgtta agacgaaccc
     7681 acaagccaaa actctgattt caacccggct ggaagtggca acacgaaaga aacgtcgtgt
     7741 gctttttatt taagccgcgc gccgcgtttt ataccccaca atggcagaaa attgcaaaag
     7801 ataaatacgc agaatgccgg cattgtcagg aaaaatttcc agccacgttt ttaacagaat
     7861 gagacacgat tcaaaaaaaa gtggaaatcg ggtgaagaat tgacctaaaa tagccatcca
     7921 gatgttaatc catccatacc gattaacact cagactgcca gtgtttttaa cctgcagagt
     7981 cgtggtagga tccgctacca cagaaaatcc acacaacagt ttgagctaac caaattctct
     8041 ttaggtgata ttaaatatgg caaaacacct ttttacgtcc gagtccgtct ctgaagggca
     8101 tcctgacaaa attgctgacc aaatctctga tgccgtttta gacgcgatcc tcgaacagga
     8161 tccgaaagca cgcgttgctt gcgaaaccta cgtaaaaacc ggcatggttt tagttggcgg
     8221 cgaaatcacc accagcgcct gggtagacat cgaagagatc acccgtaaca ccgttcgcga
     8281 aattggctat gtgcattccg acatgggctt tgacgctaac tcctgtgcag ttctgagcgc
     8341 tatcggcaaa cagtctcctg acatcaacca gggcgttgac cgtgccgatc cgctggaaca
     8401 gggcgcgggt gaccagggtc tgatgtttgg ctacgcaact aatgaaaccg acgtgctgat
     8461 gccagcacct atcacctatg cacaccgtct ggtacagcgt caggctgaag tgcgtaaaaa
     8521 cggcactctg ccgtggctgc gcccggacgc gaaaagccag gtgactttcc agtatgacga
     8581 cggcaaaatc gttggtatcg atgctgtcgt gctttccact cagcactctg aagagatcga
     8641 ccagaaatcg ctgcaagaag cggtaatgga agagatcatc aagccgattc tgcccgctga
     8701 atggctgact tctgccacca aattcttcat caacccgacc ggtcgttttg ttatcggtgg
     8761 cccgatgggt gactgcggtc tgactggtcg taaaattatc gttgatacct acggcggcat
     8821 ggcgcgtcac ggtggcggtg cattctctgg taaagatcca tcaaaagtgg accgttccgc
     8881 agcctacgca gcacgttatg tcgcgaaaaa catcgttgct gctggcctgg ccgatcgttg
     8941 tgaaattcag gtttcctacg caatcggcgt ggctgaaccg acttccatca tggtagaaac
     9001 tttcggtact gagaaagtgc cttctgaaca actgactctg ctggtacgtg agttcttcga
     9061 cctgcgccca tacggtctga ttcagatgct ggatctgctg cacccgatct acaaagaaac
     9121 cgcagcatac ggtcactttg gtcgtgaaca tttcccgtgg gaaaaaaccg acaaagcgca
     9181 gctgctgcgc gatgctgccg gtctgaagta atctttcttc acctgctttc aaaggccagc
     9241 ctcgcgctgg cctttttctt ttacctcacc gcggtggata ggcgttcacg ccgcatccgg
     9301 caaaaaaacc gcctgcacaa taacatcatt cttcctgatc acgtttcacc gcaaattatc
     9361 atcacaactg aaaccgatta caccaaccac aacagacaaa gatttgtaat atttccatat
     9421 tattattcgg ttttcacagt tgttacattt cttttcagta aagtcttaat tgcaga
//
LOCUS       DAZKIP010000149         9360 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZKIP010000149 DAZKIP010000000
VERSION     DAZKIP010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.150"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            33..107
                     /gene="tnaC"
                     /locus_tag="ACHOUK_004073"
     CDS             33..107
                     /gene="tnaC"
                     /locus_tag="ACHOUK_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFP9899416.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            328..1743
                     /gene="tnaA"
                     /locus_tag="ACHOUK_004074"
     CDS             328..1743
                     /gene="tnaA"
                     /locus_tag="ACHOUK_004074"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFP9899417.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENASNIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1834..3081
                     /gene="tnaB"
                     /locus_tag="ACHOUK_004075"
     CDS             1834..3081
                     /gene="tnaB"
                     /locus_tag="ACHOUK_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFP9899418.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFLVDYSILRDATSSTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3213..4388
                     /gene="mdtL"
                     /locus_tag="ACHOUK_004076"
     CDS             3213..4388
                     /gene="mdtL"
                     /locus_tag="ACHOUK_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFP9899419.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIITSVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMAIMGIAVLMLSLFILKETRPAAPAASDKSRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLTVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGISAWNMLIGILIACSIVSLLLIMFVAPGRPVTAHEEIHHHA"
     gene            4363..5322
                     /gene="yidZ"
                     /locus_tag="ACHOUK_004077"
     CDS             4363..5322
                     /gene="yidZ"
                     /locus_tag="ACHOUK_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFP9899420.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKQIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHEKWDLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRDTIKNLYASMA"
     gene            5479..6228
                     /gene="yieE"
                     /locus_tag="ACHOUK_004078"
     CDS             5479..6228
                     /gene="yieE"
                     /locus_tag="ACHOUK_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFP9899421.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWVSKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTMKAFSPN"
     gene            6250..6816
                     /gene="yieF"
                     /locus_tag="ACHOUK_004079"
                     /gene_synonym="chrR"
     CDS             6250..6816
                     /gene="yieF"
                     /locus_tag="ACHOUK_004079"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFP9899422.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6870..8207)
                     /gene="adeP"
                     /locus_tag="ACHOUK_004080"
     CDS             complement(6870..8207)
                     /gene="adeP"
                     /locus_tag="ACHOUK_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFP9899423.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRIRDLSPCVIIVALLFILKIVFIDAH"
     gene            8373..9038
                     /gene="yieH"
                     /locus_tag="ACHOUK_004081"
     CDS             8373..9038
                     /gene="yieH"
                     /locus_tag="ACHOUK_004081"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFP9899424.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGIHVDLKEIFT
                     RFKGVKLYEIIDIISKEQGVTMVQADAEHVYRAEVARLFDTELEDIAGANELLASINV
                     PMCVVSNGPVSKMQHSLGKLNMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTELSQLPALWKARGWN
                     ITR"
     gene            complement(9175..>9360)
                     /locus_tag="ACHOUK_004082"
     CDS             complement(9175..>9360)
                     /locus_tag="ACHOUK_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899425.1"
                     /translation="VNALNAKIVDCDDKIRSKLTIVRNNRLENQYKDDKNISDAMRNI
                     LDWFERYPDIVQNITQA"
BASE COUNT         2304 a         2330 c         2324 g         2402 t
ORIGIN      
        1 gtagcttcgt gtctttcgag gataagtgca ttatgaatat cttacatata tgtgtgacct
       61 caaaatggtt caatattgac aacaagattg tcgatcaccg cccttgattt gcccttctgt
      121 agccatcacc agagccaaac cgattagatt caatgtgatc tatttgtttg ctatatctta
      181 aatttgcctt ttgcaaaggc catctctcgt ttatttactt gtgttagtaa atgatggtgc
      241 ttgcatatat acatggcgaa ttaatggata ttgcagatgc aatattcaca gggatcactg
      301 taattaaaat aaatgaagga ttatgtaatg gaaaacttta aacatctccc tgaaccgttc
      361 cgcattcgtg ttattgagcc agtaaaacgt accactcgcg cttatcgtga agaggcaatt
      421 attaaatccg gtatgaaccc gttcctgctg gatagcgaag atgtgtttat cgatttactg
      481 accgacagcg gcaccggggc ggtgacgcag agtatgcagg ccgcgatgat gcgcggcgac
      541 gaagcttaca gcggcagccg cagctactat gcgttagccg agtcagtgaa aaatatcttt
      601 ggttatcaat acaccattcc gactcaccag ggccgtggtg cagagcaaat ctatattcca
      661 gtactgatta aaaagcgcga gcaggaaaaa ggcctggatc gcagcaaaat ggtggcgttc
      721 tctaactatt tctttgatac cacgcagggc catagccaga ttaacggctg taccgtgcgt
      781 aacgtctata tcaaagaagc cttcgatacg ggcgtgcgtt acgactttaa aggcaacttt
      841 gaccttgagg gattagaacg cggtattgaa gaagttggtc cgaataacgt gccgtatatc
      901 gttgcaacca tcaccagtaa ctccgcaggc ggtcagccgg tttcactggc aaacttaaaa
      961 gcgatgtaca gcatcgcgaa gaaatatgat attccggtgg taatggactc cgcgcgcttt
     1021 gctgaaaacg cctatttcat caagcagcgt gaagcagaat acaaagactg gaccatcgag
     1081 cagatcaccc gcgaaaccta caaatatgcc gatatgctgg cgatgtccgc caagaaagat
     1141 gcgatggtgc cgatgggcgg cttgctgtgc atgaaagacg acagtttctt tgatgtgtac
     1201 accgagtgca gaaccctttg cgtagttcag gaaggcttcc cgacgtatgg cggcctggaa
     1261 ggcggcgcga tggagcgtct ggccgtaggt ctgtatgacg gcatgaatct ggactggctg
     1321 gcttatcgta ttgcgcaggt gcagtatctg gtcgatggtc tggaagagat tggcgttgtc
     1381 tgccagcagg cgggcggtca cgcggcattc gttgatgccg gtaaactgct tccgcatatc
     1441 ccggcagatc agttcccggc acaggcgctg gcctgcgagc tgtataaagt cgccggtatc
     1501 cgcgcggtag aaattggctc tttcctgtta ggccgcgatc cgaaaaccgg taaacaactg
     1561 ccatgcccgg ctgaactgct gcgtttaacc attccgcgcg caacatatac tcaaacacat
     1621 atggacttca ttattgaagc cttcaaacat gtgaaagaga acgcgtcgaa tattaaaggg
     1681 ttaaccttta cctacgaacc aaaagtattg cgtcacttca ccgcaaaact gaaagaagtt
     1741 taattaatac tactgagtgg ctataaggat gttagccact ctcttaccct acatcctcaa
     1801 taacaaaaat agccttcctc taaaggtggc atcatgactg atcaagctga aaaaaagcac
     1861 tctgcatttt ggggtgttat ggttatagca ggtacagtaa tcggtggagg tatgtttgct
     1921 ttacctgtgg atcttgccgg tgcctggttt ttctggggtg cctttatcct tatcattgcc
     1981 tggttttcaa tgcttcattc cgggttattg ttattagaag caaatttaaa ttatcccgtc
     2041 ggctccagtt ttaacaccat caccaaagat ttaatcggta acacctggaa cattatcagc
     2101 ggtattaccg ttgccttcgt tctctatatc ctcacttatg cctatatctc tgctaatggt
     2161 gcgatcatta gtgaaacgat atcaatgaat ttgggttatc acgctaatcc acgtattgtc
     2221 gggatctgca cagccatttt cgttgccagc gtattgtgga taagctcgtt agccgccagt
     2281 cgtattacct cattgttcct cgggctgaag attatctcct ttgtgatcgt gtttggttct
     2341 ttcttcttcc tggtcgatta ctccattctg cgcgatgcca ccagctccac tgcgggaacg
     2401 tcttacttcc cgtatatctt tatggctttg ccggtgtgtc tggcgtcatt tggtttccac
     2461 ggcaatattc ccagcctgat tatttgctat ggcaaacgca aagataagct aatcaaaagc
     2521 gtggtcttcg gttcgctgct ggcgctggtg atttatctct tctggctcta ttgcaccatg
     2581 gggaatattc cgcgagaaag ctttaaggcg attatttcct caggcggcaa cgttgattca
     2641 ctggtgaaat cgttcctcgg caccaaacag cacggcatta tcgagttttg cctgctggtg
     2701 ttctccaact tagctgtcgc cagctcgttt tttggtgtca cgctgggatt gttcgattat
     2761 ctggcggacc tgtttaagat tgataactcc cacggcgggc gtttcaaaac cgtgctgtta
     2821 accttcctgc cacccgcgtt gttgtatctg atcttcccga acggctttat ttacgggatc
     2881 ggcggtgccg gactgtgcgc cactatttgg gcggtcatta ttcccgcagt gctggcaatc
     2941 aaagctcgca aaaagtttcc caatcagatg ttcacggtct ggggcggcaa tcttattccg
     3001 gcgattgtca ttctctttgg tataaccgta attttgtgct ggttcggcaa cgtctttaac
     3061 gtgttaccta aatttggcta aatccttcaa gaagccagtc attcgctggc ttcttgcctc
     3121 tcaggaaatc acttatgtcc aaatggcaac tcgcctgatc ctccttcacc acgtatgctt
     3181 tgcgtcacct tactatcagg acgctttagc ccatgtcccg ctttttgatt tgtagttttg
     3241 ccctggtttt actttatccc gctgggattg atatgtacct cgttggttta ccgcgcatcg
     3301 ccgccgatct caatgccagc gaagcgcagt tacatattgc gttctccgtt tatctggcag
     3361 gtatggcagc tgcgatgtta tttgccggta aagtggccga tcgttcaggg agaaaaccgg
     3421 tcgccatccc cggcgcggcg ctatttatta tcacttcggt gttctgttca ctggctgaaa
     3481 ccagcacgtt atttcttgca gggcgctttc tacaggggtt gggcgcgggc tgttgttacg
     3541 tggtggcatt cgccattttg cgcgacacgc tggatgatcg acgtcgggca aaagtgctgt
     3601 cgttactcaa cggcattacc tgcatcattc cggtgttagc gccggtgctc ggtcatctga
     3661 ttatgcttaa attcccgtgg cagagtctgt tctggacgat ggcaataatg ggcatcgcgg
     3721 tactgatgtt gtctttgttt attttaaaag aaacgcgccc tgccgccccc gcagcttcgg
     3781 acaaatcacg agaaaatagc gagtcgctgc ttaatcggtt tttcctcagc cgtgttgtta
     3841 tcaccaccct cagcgtttcg gtgatcctca ccttcgtcaa tacatcgccg gtattgctga
     3901 tggaaatcat gggttttgag cgcggagaat acgccaccat tatggcgttg accgctggcg
     3961 tcagcatgac cgtttcattt tccacgccat ttgcgctggg aatttttaag ccacgtacgt
     4021 tgatgatcac ctcacaggtg ttattcctgg cggcgggcat cactcttacc gtttcacctt
     4081 cccatgcggt ttctctgttt ggtatcacgc tgatttgcgc cggtttctcg gtaggttttg
     4141 gtgtggcgat gagtcaggcg ttagggccgt tttcattacg cgcgggcgta gccagctcga
     4201 ccttaggtat tgcgcaggtt tgcggttcgt cactgtggat ttggctggca gcggtggttg
     4261 gtatcagcgc atggaatatg ctgatcggga ttctgattgc ctgtagcata gtgagcctgt
     4321 tgctgattat gttcgtcgcg cctggacgcc cggttaccgc tcatgaagaa atccatcacc
     4381 acgcttgatc tcaatctgct gctctgtctg cagctgctga tgcaggagcg cagcgtaacc
     4441 aaagcggcga agcggatgaa cgtgacacct tcggcggtga gtaagtcgct ggcaaagtta
     4501 agagcgtggt ttgacgaccc gctctttgtg aactcaccgt tggggctgtc gcccacaccg
     4561 ctaatggtca gcatggagca aaatctggcg gagtggatgc aaatgagcaa cctgctgctg
     4621 gataaaccgc accaccagac accgcgcggc ctgaagtttg agctggcggc agaatcaccg
     4681 ctaatgatga tcatgcttaa tgcgctgtcg aaacagatct accaacgtta cccgcaggcg
     4741 accatcaaat tacgtaactg ggattacgat tccttagatg ccattactcg tggtgaagtg
     4801 gatatcggtt tttccggtcg cgaaagtcat ccccgttcgc gggagctgtt aagctcgcta
     4861 ccgttagcca ttgattatga agtgttgttt agtgatgtgc cctgcgtctg gttacgcaaa
     4921 gatcatccgg cactgcatga aaagtgggat ctcgatacct ttttacgtta tccgcatatc
     4981 agcatttgct gggaacagag cgatacctgg gcgctggaca atgtgttaca ggagctgggg
     5041 cgcgaacgta cgattgcaat gagcctgccg gaattcgagc agtcactgtt tatggcagcg
     5101 cagcccgaca atctgctcct ggcgaccgcg ccgcgctact gtcagtacta caatcaactc
     5161 caccaactgc cgttggttgc tcttcctctc ccgtttgacg aaagccagca aaaaaagctg
     5221 gaagtccctt ttaccctgct gtggcataaa cggaacagcc ataatccgaa gatcgtctgg
     5281 ttacgggata ccattaaaaa tctttacgcg tcgatggcat aaccgaatcg tatgaaacgc
     5341 gacccgattt cacaataaaa tgtaaaaaag ttgtaataag cttgtctgaa tcgaactttt
     5401 agccgcttta gtctgtccat cattccagta aatgattact cttgtattca taatggacca
     5461 ttaagcatgg agcgaaaaat ggcgactcac tttgcccgag ggattttaac ggaaggacat
     5521 ctgatttctg ttcgtctccc ctcccagtgt catcaagaag cccgaaacat tccccctcat
     5581 cgtcaaagcc gttttctggc gtccagaggt ttactcgcag aactgatgtt catgctgtat
     5641 ggcattggcg aattgccgga aatcgtcacc ctgccgaaag gtaaaccggt tttcagtgat
     5701 aaaaatttgc cttcgttttc tatttcctat gccgggaata tggttggcgt ggcgttaaca
     5761 accgaaggtg aatgtggcct cgatatggaa ctgcagcgtg cgacgcgcgg gtttcatagc
     5821 ccacacgcgc ccgataacca cacattttcc agcaatgaat cgctatgggt cagtaaacaa
     5881 aatgatccaa acgaagcgcg ggcgcagctc atcacgctgc gccgaagcgt gctaaaacta
     5941 accggtgatg ttttgaatga cgatccgcgc gatctgcaac tgctgcccat tgccggacgc
     6001 ctgaaatgtg ctcatgtaaa tcatgtagaa gcgttatgcg acgcggagga cgtgctggtg
     6061 tggtccgtgg cggtcacgcc cacgattgaa aagctcagtg tctgggagtt agatggcaaa
     6121 cacggctgga aaagcctgcc ggatattcac agccgcgcca acaatcctac cagccggatg
     6181 atgcgttttg cccagctctc taccatgaag gctttttcgc caaattgata gacaaccaca
     6241 ggagtcatta tgtctgaaaa attgcaggtg gttacgttac tggggagcct gcgcaaaggc
     6301 tcatttaatg gcatggttgc acgtacccta ccgaaaattg ctccggcgag catggaagtc
     6361 aatgcgttac cttccattgc cgacattccc ctgtatgacg ctgacgtaca gcaggaagaa
     6421 ggttttccag caacggttga agctctggcg gaacagatcc gtcaggctga cggtgtggtg
     6481 atcgtcacgc cggaatataa ctattcggta ccgggtgggc tgaaaaatgc catcgactgg
     6541 ctttcccgcc tgccggatca accgctggcc ggtaaaccgg tgttgattca gaccagctca
     6601 atgggcgtga ttggcggcgc gcgctgtcag tatcacctgc gccagattct ggttttcctc
     6661 gatgcaatgg tgatgaacaa gccggaattt atgggcggcg tgattcagaa caaagttgat
     6721 ccgcaaaccg gagaagtgat tgatcagggt acgctggacc acctgaccgg gcaattgacc
     6781 gcatttggcg agtttattca gcgagttaag atctaaataa aaaacccgcc agcaatcatg
     6841 catggcgggt ttttaacgcg ctatcgattt tagtgagcgt cgataaatac aatcttcagg
     6901 ataaacagca gcgcaacgat gattacgcac gggctaaggt cacgaatacg cccggtacca
     6961 atcttcatca cgcagtagga gataaagccc agcgcaatac cttcggtaat cgagaagctg
     7021 aacggcatca tcacagcggt aataaacgcc ggaacagatt cagtaagatc ctgccagttc
     7081 acgcgagcaa gacttgaggt catcaacacg ccaacgtaaa tcagcgcgcc agctgcagcg
     7141 tagcctggca ccattcccgc cagcggcgac agaaagataa ccagcaggaa cagcagacca
     7201 acaaccactg ccgtcagacc ggtacgaccg ccaaccgata cgccggaaga ggactcaata
     7261 taagccgtaa cggaagaagt accgataaac gaaccggtca cggaagagat actgtcgaca
     7321 tacagcgcct gcttcatgcg cgggaatttc cccttctcat ctgccagacc ggctttatcg
     7381 gtcacgccaa tcaacgtgcc ggaggagtca aacaggttga ccagcatgaa agagaaaatc
     7441 acccctgcca gcccgaggtt aaacgacccg gctaaatcta catgacccac gacagtcatt
     7501 acgctcggcg gcgcagaaac gatgccattg tagtggacat cacccagcat ccagcccagc
     7561 agcgttgtca ccacgataga aaccagcacc gctgcgtgaa tgttgcgtga ggccagaata
     7621 gcaatgatga agaagccgag gatccccagc agcacgctgt gagaagtcag attaccgatg
     7681 ctgaccagcg tttccgggtt agcgacaatc acacctgcgt ttttcagccc catcatgcca
     7741 atgaacagac cgataccgct ggtaatgccc acacgcagac tcaccggaat gttggcaatc
     7801 atccagtagc gaacgcggaa aatcgtcagt aacagcagac caatcgcgcc ccagaagatt
     7861 gcgcccatcc cgacttgcca cggcaagccc atcgcctgca caacgacaaa agcgaagaac
     7921 gcattcaggc ccatagcggg tgccagtgca actggcaggt tagcaaacag ccccatcata
     7981 atactgccga atgcagcaat cagacaggta gtgacgaaga cggcgctggt atccatgcca
     8041 gcaacgccaa gaatttgcgg gttaacaaaa acgatgtaaa ccatcgtcag gaaggtggta
     8101 aaaccggcga tcacttcggt ccgtgccgtc gtgccatgtt cgcgcagttt aaacacgcgt
     8161 tccagcatcc cctggccaga agcctgggtt gtgtgttgtt gactcattat ctatttccga
     8221 acaaggaggg aaaatccgtc gctatcgtat accaaaatga gacaatatgg acggctgaag
     8281 ataacttttt tcattgtatt tgcttatacg gcaacgattg cgtcatgcga attggcatga
     8341 cgaaaacgtt aaactaacga taaaggaaag acatgtccca gatagaagcg gtatttttcg
     8401 actgcgacgg tacgctggtc gatagcgagg tcatttgctc tcgcgcctat gtcacgatgt
     8461 tcagggaatt tggcattcac gttgacctga aagagatttt tacccgtttt aaaggcgtaa
     8521 aactctacga aatcatcgat atcatcagta aagaacaggg cgtgacgatg gtccaggccg
     8581 atgccgagca cgtttaccgc gcagaagtag cacgtctgtt tgataccgaa cttgaggaca
     8641 tcgcaggagc gaacgaattg ctggcgagca ttaacgtgcc gatgtgcgtc gtctctaacg
     8701 gtccggtcag caaaatgcag cattcgcttg gtaagctcaa catgctgcac tacttcccgg
     8761 aaaaactgtt cagcggatat gacattcagc gttggaagcc cgatccggca ttaatgttcc
     8821 atgcggcaaa agcgatgaat gtgaacgttg aaaactgcat tctggtcgat gactccagcg
     8881 ctggcgctca atcagggata gatgccggaa tggaagtgtt ttacttctgc gctgacccgc
     8941 acaataagcc gatcgttcac ccgaaagtca ccacctttac cgagctgtcg cagctgcctg
     9001 cgctctggaa ggcacgcggc tggaatataa cgcgataatc gcctccttac ttcaacactt
     9061 acacatcagt cacgctggtt tgagatacag aacaccaggt gtctgatgag taagcgtcta
     9121 ttccctatga ataatttatg tcgttattcg tcctgtcatc caccctctgt atggttaagc
     9181 ctgggttata ttttgtacaa tatcgggata ccgttcgaac caatccagaa tattacgcat
     9241 cgcgtcactg atgtttttat cgtctttata ttgattctca agtctattat ttcgaacaat
     9301 agtaagctta gaacgtattt tatcatcaca atcgacaatt ttcgcattca atgcatttac
//
LOCUS       DAZKIP010000150         9196 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZKIP010000150 DAZKIP010000000
VERSION     DAZKIP010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.151"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(51..1427)
                     /locus_tag="ACHOUK_004083"
     CDS             complement(51..1427)
                     /locus_tag="ACHOUK_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFP9899426.1"
                     /translation="MRQDIEASVIGGLLIGGLTPTASDVLATLEPEAFSIPLYRKAFE
                     VIRKQARNRNLIDALMVAEACGEEHFTSILMTSKNCPSAANLKGYAGMVADNYHRRLV
                     LEIMDEMREPIQSGTIDASSQAMDELVKRLSAIRKPRDEVKPVRLGEIITDYTDTLDR
                     RLRNGEESDTLKTGIEELDAITGGMNAEDLVIIAARPGMGKTELALKIAEGVASRVIP
                     GSDVRRGVLIFSMEMSALQIAERSIANAGRMSVSVLRNPASMDDEGWARVANGMSQLA
                     DLDVWVVDASRLSVEEIRSIAERHKQENPNLSLIMADYLGLIEKPKADRNDLAIAHIS
                     GSLKAMAKDLKTPVISLSQLSRDVEKRPNKRPTNADLRDSGSIEQDADSIIMLYREAV
                     YDENSSAAPFAEIIVTKNRFGSLGTVYQRFCNGHFVACDQDEARQICTASNAPAARGR
                     RYAQGADV"
     gene            complement(1424..2245)
                     /locus_tag="ACHOUK_004084"
     CDS             complement(1424..2245)
                     /locus_tag="ACHOUK_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFP9899427.1"
                     /translation="MGVVKLADYRPQLEVVEHRVADTEDGFMRVANEITDSLLMADLT
                     VRQLKVMLAIMRKTYGFNKPMDRLTNTQIAAMTGIHHTHVCAAKRQLIERKFLIADGV
                     KIGVNKVVSQWISQDSLTLAKTANKTLAKSANGYKPSQLNTKDNIQKTINTNTPLPPN
                     GDGDGQVKPERRKAERIDYESFLNAYNTEVGDRLPHAVAVNEKRKRRLKKIIPQLKTP
                     NVDGFRAYVRAFVHQAKPFYFGDNDTGWTADFDYLLREDSLTGVREGKFADRGIA"
     gene            complement(2232..2393)
                     /locus_tag="ACHOUK_004085"
     CDS             complement(2232..2393)
                     /locus_tag="ACHOUK_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899428.1"
                     /translation="MTKRRKKYQEKEEIRHPDSPEGLVVAAANNRAFAERLVGVYRLA
                     KAGVKHGRR"
     gene            complement(2426..2722)
                     /locus_tag="ACHOUK_004086"
     CDS             complement(2426..2722)
                     /locus_tag="ACHOUK_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFP9899429.1"
                     /translation="MERTSYSKLSQRDVDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDEITKKKSPAATEDFKQIDMQF"
     gene            complement(2864..3079)
                     /locus_tag="ACHOUK_004087"
     CDS             complement(2864..3079)
                     /locus_tag="ACHOUK_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9899430.1"
                     /translation="MSNLRKYRESLNISQTTLAKAVGCTQGAIGHWESGRRFPDLKTC
                     RALVACLNKLGAKVSLDDVFPPEHKAA"
     gene            3154..3849
                     /locus_tag="ACHOUK_004088"
     CDS             3154..3849
                     /locus_tag="ACHOUK_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFP9899431.1"
                     /translation="MDGSSTERNNKDMKMKWYELARSRMKELGITQEKLAEELGMTQG
                     GIGHWLRGSRHPSLSDIGVVFKYLGIDNISFNHDGTFSPVGEYSSAPVKKQYEYPVFS
                     HVQAGMFSPELRTFTKGDAERLVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPDGML
                     ILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSVVG
                     KVIASQWPEETFG"
     gene            4351..4872
                     /locus_tag="ACHOUK_004089"
     CDS             4351..4872
                     /locus_tag="ACHOUK_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899432.1"
                     /translation="MAKSNVSVQAFKDFLEELMSLNIMKEATARNLKNSSARLLTVVQ
                     EEEMGDVTQLDVNELAERYINATEPKPSDSSITAYKSRMESAIKKFVAFQSGEEIPYT
                     PIDKESSEEKDLTGEPTKVEGKANALHTYDLPVVLRPESGVTVTIKGIPNDITNEEAE
                     RISSILKVYVRPQ"
     gene            5441..5623
                     /locus_tag="ACHOUK_004090"
     CDS             5441..5623
                     /locus_tag="ACHOUK_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899433.1"
                     /translation="MEQTGRLFKQRRLSTTWLKSQITQPHKLWDAMPKQPSQEELRDC
                     IAKVYSGGIYVQKNRI"
     gene            5601..5873
                     /locus_tag="ACHOUK_004091"
     CDS             5601..5873
                     /locus_tag="ACHOUK_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HFP9899434.1"
                     /translation="MSRKTEFKGTAASRRRARRANLQSQEAISSDKLHRPTPSRVVLQ
                     CKRKPAMRAEVITLTTLTRKYEGSTCLPNVALYAAGYRKSKQLTAR"
     gene            5932..6183
                     /locus_tag="ACHOUK_004092"
     CDS             5932..6183
                     /locus_tag="ACHOUK_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899435.1"
                     /translation="MEFHESAICDFRANANSVKPQPIAVLFKTMGAWAVLCFAADDTD
                     ARMAIGQEMEMDPTNDEFIIYGAPSNYLLDTCNIYNKAA"
     gene            6366..6734
                     /locus_tag="ACHOUK_004093"
     CDS             6366..6734
                     /locus_tag="ACHOUK_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2528 family protein"
                     /protein_id="HFP9899436.1"
                     /translation="MSNIKKYIIDYDWKASIEIEIDHDVMTEEKLHQINNFWSDSEYR
                     LNKHGSLLNAVLIMLAQHALLIAISSDLNAYGVVCEFDWNDGNGQEGWPPMDGSEGIR
                     ITDIDTSGIFDSDDMTIKAA"
     gene            6807..6971
                     /locus_tag="ACHOUK_004094"
     CDS             6807..6971
                     /locus_tag="ACHOUK_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HFP9899437.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDILNQPGVPK
                     NGSNTYGYPD"
     gene            6940..7083
                     /gene="kil"
                     /locus_tag="ACHOUK_004095"
     CDS             6940..7083
                     /gene="kil"
                     /locus_tag="ACHOUK_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFP9899438.1"
                     /translation="MDQTLMAIQTKFTIATFIGDEKMFREAVDAYKKWILILKLRSSK
                     SIH"
     gene            7158..7454
                     /locus_tag="ACHOUK_004096"
     CDS             7158..7454
                     /locus_tag="ACHOUK_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFP9899439.1"
                     /translation="MNAYYIQDRLEAQSWTRHYQQIAREEKEAELADDMGKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            7460..8245
                     /gene="bet"
                     /locus_tag="ACHOUK_004097"
     CDS             7460..8245
                     /gene="bet"
                     /locus_tag="ACHOUK_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFP9899440.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKVLGFLKQKASEQKVAA"
     gene            8242..8922
                     /locus_tag="ACHOUK_004098"
     CDS             8242..8922
                     /locus_tag="ACHOUK_004098"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFP9899441.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVIE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            8919..9101
                     /locus_tag="ACHOUK_004099"
     CDS             8919..9101
                     /locus_tag="ACHOUK_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFP9899442.1"
                     /translation="MKHPHDNIRVGAITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKRGAVCTKHLPLS"
     gene            9074..>9196
                     /locus_tag="ACHOUK_004100"
     CDS             9074..>9196
                     /locus_tag="ACHOUK_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFP9899443.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLA"
BASE COUNT         2504 a         2186 c         2153 g         2353 t
ORIGIN      
        1 aagaaccatt agtaacgata accctgctat tagctcagtg atgtagatgg tcatacgtca
       61 gccccttgtg catatcgtct gccacgcgca gcaggtgcat ttgatgctgt gcaaatctgt
      121 ctggcttcat cctggtcaca tgcaacaaag tgtccgttac agaaccgctg gtaaactgta
      181 ccaagcgagc caaaacggtt tttcgtcacg atgatttcag caaatggcgc ggcgctactg
      241 ttctcatcat ataccgcttc ccgatagagc atgatgattg agtctgcgtc ctgttcaatg
      301 cttcctgaat cacgcaaatc tgcgtttgtc gggcgtttgt ttggtcgctt ctcaacatcg
      361 cgcgaaagct gactcaggga gataaccggt gttttcaggt ctttcgccat cgccttcagg
      421 cttccggaga tgtgagcaat tgcgaggtcg ttgcggtctg ctttcggctt ctcaatcagg
      481 ccaagataat ccgccatgat gagtgacagg tttggatttt cctgcttatg ccgttctgcg
      541 attgagcgta tttcttcgac cgataaccgc gaagcatcga ctacccatac atccaaatct
      601 gcaagctgac tcatgccgtt agcaacacgt gcccagccct cgtcatccat cgatgcagga
      661 tttcgcagta cgctaaccga catcctcccg gcgttggcaa tgcttcgctc tgcaatctgc
      721 aatgcgctca tttccattga gaaaatcaat accccgcgcc ggacgtcaga accaggaata
      781 acgcggcttg ccacaccttc ggcaatcttc agcgccagtt cggttttccc cataccagga
      841 cgagcggcga taatcaccag gtcttctgcg ttcatccctc cagtgatagc gtcaagctct
      901 tcgattccgg tcttcagggt atctgactct tctccgttcc tcagacgcct gtcaagcgtg
      961 tcagtgtagt cagtgatgat ttcccctaac cgtacaggtt taacctcgtc acggggcttt
     1021 ctgatggctg aaagacgctt tacaagttca tccatcgcct gactcgatgc gtcgatggtt
     1081 ccgctttgga ttggttcacg catttcatcc atgatttcca gcaccagacg gcggtgatag
     1141 ttatccgcga ccattccggc atatcccttc aggtttgcgg cactcgggca gtttttgctg
     1201 gtcatcagga ttgacgtgaa atgctcctct ccgcacgcct cggcaaccat cagcgcatcg
     1261 attaggtttc tgtttctcgc ctgcttgcga ataacctcga aggctttccg gtagagcgga
     1321 attgaaaacg cttccggctc cagcgttgcc agaacgtcac tggcggttgg agttaatcca
     1381 ccaatcagca ggccaccgat aacgctagct tcgatatcct gtctcatgca atccccctgt
     1441 ctgcaaactt cccttcccga actcccgtta acgaatcttc cctcagcagg taatcaaaat
     1501 ctgccgtcca gcccgtgtcg ttgtctccga agtaaaacgg cttggcctga tgcacaaacg
     1561 ccctgacata cgctctgaaa ccgtccacgt ttggcgtttt cagttgcggg ataattttct
     1621 tcaggcggcg ttttcgtttc tcgttgaccg caacagcgtg tggaagtctg tcaccgactt
     1681 cggtgttgta ggcgttcagg aaggattcgt agtcgattcg ttctgccttg cgacgttcag
     1741 gtttaacctg cccatcgcca tccccgttag ggggtaaggg ggtatttgta tttattgtct
     1801 tttgtatatt gtcttttgtg tttagctgac ttggcttata cccattagcc gacttggcta
     1861 atgttttatt agctgtttta gctaatgtta agctgtcctg gctaatccac tgagaaacca
     1921 ccttgttcac tccgattttc acgccatcag caatgaggaa tttacgctca ataagctggc
     1981 gcttggcagc gcaaacatga gtgtgatgaa tacctgtcat ggctgctatc tgcgtgtttg
     2041 tgagtcgatc catcggctta ttgaatccgt atgtcttgcg catgatagcg agcatcacct
     2101 tcaactgccg gacggttaaa tcagccatca gcagactgtc ggtaatctcg ttagcaacgc
     2161 gcatgaaacc atcttcggta tctgccacgc gatgctccac gacctccagt tgaggcctgt
     2221 aatcagctaa cttaacgacg cccatgtttc actcctgctt tggctagtct gtaaacacca
     2281 acaaggcgct ctgcgaacgc cctgttattt gctgcggcta ccactaatcc ctcaggtgaa
     2341 tcagggtgtc gaatctcttc tttttcctgg tatttcttac gacgttttgt cataattact
     2401 cctgtggatt gatccagtaa ttccctcaga attgcatatc aatttgctta aaatcctcgg
     2461 tggcagccgg ggattttttc tttgtgattt catcaagcgc atacttaaaa gccctgctaa
     2521 tcggactgat gtctgatgcc attccgaaag cacacaagac cgaagcaata aaccgccagt
     2581 ccgttctgct tatcttcgat tcatgacagc caatcatctt tgccagaccg cgctgtgtaa
     2641 gcgttgacag gttgatgagt aaatctgttt ctgcgcgatc aacgtcgcgc tgtgatagtt
     2701 tgctgtaact tgttcgttcc atttcttaag atttccaata gtgaatagtt agttgaaagg
     2761 tatgcgtgga aacgcatatg gccttagttg gtcagatatc ttgggactcg cttttcagcg
     2821 acgtaggacg aatgtccgtt gttacaaaga gcggctccgc ttattaagcg gctttgtgct
     2881 ccggcgggaa cacgtcatca agacttactt ttgcgcctaa cttgtttaga cacgcaacaa
     2941 gagcacggca tgttttaagg tctgggaagc gacgaccaga ttcccaatgt ccgatagctc
     3001 cctgtgtgca tccaactgcc ttagcaagtg ttgtttgaga gatattcagt gactctcgat
     3061 attttcgtag gttgctcata tgccctccat agtaaccacg aataaaaaat acaatatgta
     3121 cttctcaaat acaagtaaaa atacacattg tgcatggatg gttccagtac agagcgtaat
     3181 aataaggaca tgaaaatgaa atggtatgaa ctggctagat ccagaatgaa agagctcggc
     3241 ataactcaag agaagttagc cgaagagcta ggtatgacgc agggtggaat tggacactgg
     3301 ttgcgcggat ctcgtcatcc atctcttagt gatattggtg tggtgtttaa ataccttggt
     3361 attgataaca tatcattcaa ccacgacggg acattttcac ctgttggcga atactcatcg
     3421 gccccagtta aaaaacaata tgagtaccct gttttttctc atgttcaggc tgggatgttc
     3481 tctccagaac tcagaacctt taccaaaggt gatgcggaga gattggtaag cacaaccaaa
     3541 aaagccagtg actctgcatt ctggcttgag gttgaaggta actcaatgac cgcaccaaca
     3601 ggttccaaac ctagttttcc tgacgggatg ttaattctgg ttgaccctga gcaagctgtt
     3661 gagcccggcg atttctgcat agccagactt ggtggtgatg aatttacctt caagaaactg
     3721 atcagggata gcggtcaggt gtttctacaa ccactaaacc cgcaatatcc aatgatccca
     3781 tgcaatgaga gttgttccgt tgtggggaaa gttatcgcca gccaatggcc tgaagagacg
     3841 tttgggtaaa gaggatagat ggagtttaat ggccatgagt atcaagcggt caaaaagaag
     3901 ttcatctatt cattgaaagc ataaggccat ctgagtatat ccgtaatgat ctggatattg
     3961 tctataacat caatgaccgg acgatagata tcggtgagca gcgtcctgta tggcagggtg
     4021 aggcaggtga aaaaacgtcc tgccatcaac aagaatcaag tgcatccgct ctctggacag
     4081 atggaaaatt tattggatgc aaaaagatat gaaataacac ctgtatagca cggagctttc
     4141 tctgaccgat gcacttgaag ttgttagggc tgacccagcc tgctgtttct ttaggtaaga
     4201 gaaaagagat ttaggagatg aaaggtcgca gaggtgcggc cttttttatt gagagtggat
     4261 cttgaacgga aattctcaag acttgagtct tgcatgcaaa tcaattcctg gataaactcg
     4321 atctgagtca ataacttaga gagagagcaa atggcaaagt caaacgttag cgtgcaggca
     4381 ttcaaggact tccttgaaga gcttatgtcg ctgaacataa tgaaggaggc caccgctcga
     4441 aatttaaaaa actcatccgc tcgcctctta acggtagtcc aagaagagga aatgggtgat
     4501 gttactcagc ttgatgtgaa tgagcttgcc gagcgataca tcaacgcaac tgagccgaag
     4561 cctagcgaca gcagcattac tgcatataaa agccgcatgg aaagtgcaat caaaaagttt
     4621 gtagctttcc agtctggtga agaaatccca tacactccga ttgacaaaga atccagtgag
     4681 gaaaaagatt tgactggcga accaacaaaa gtcgaaggca aggctaatgc acttcatacc
     4741 tatgatcttc cagtagttct tcgacctgaa tcaggggtta cagtaacgat taaaggcatt
     4801 cctaacgata tcacaaacga agaagccgaa cgcatctctt caattctgaa ggtttacgtt
     4861 cggcctcaat aatgcaaaag cattcagcac aatgtccaac tccccagtcc gacattgatg
     4921 ctgtttaacc agagcctcca aaaggagccc ttgttaaggt acacaatatt tgcgatgtaa
     4981 ccttagcgcg tctggtacat tttttcaagc ggttgtaggg ctgccgccaa tatgagaaaa
     5041 cacagatgtc tacatacaat ttgaatgacc aattcgatcg agaagttcat gtgaatgcct
     5101 atgaacggat aaggcatgga aatcttgaac atgtgtgtga gcattatcgc tcaaggccac
     5161 accgctaatc atcaacccgg cctccatgcc gggttttctt ttcctctcgc ccaaaaaaac
     5221 acataaccaa ttgtatttat tggaaaataa atagatacaa ctcactaaac aacgcaattc
     5281 tgatctctcc ttacatcgcc gaggcaatac acccacgcta aaaaacaata ctattaaata
     5341 caaagcgttg taaaaaaacg ccccgtttta gaacaaattg tattgaccaa gtgaagtaca
     5401 tatcgtacta tttaaccatc agcaggacgc tggaagccaa atggaacaga ctggcaggct
     5461 ctttaaacaa cgtcgactct cgactacgtg gctgaaaagc cagatcaccc aaccacataa
     5521 gctgtgggat gcaatgccga agcaaccgtc tcaggaggag cttcgagatt gcatcgccaa
     5581 agtttattcg ggaggaatct atgtccagaa aaacagaatt taaaggcacc gcagcttctc
     5641 gccgtagagc tcgtcgcgca aacctgcaaa gtcaggaggc gattagctcc gacaaactac
     5701 acaggccaac cccatcacga gtggtcttac aatgcaaacg caaaccagca atgagagcag
     5761 aagtaataac actgacaacg ttgaccagaa aatatgaagg ctcaacttgt cttccaaatg
     5821 tagctcttta cgcggcaggc taccgtaaat ccaaacaact gacagcaaga tgacttgtgt
     5881 tggtcgccag aaaatgaaat taggcagcaa accacttatt tgaggtgata tatggaattt
     5941 catgaaagtg cgatttgtga ttttcgcgct aacgcaaatt cagtaaaacc acagccaatt
     6001 gcagttcttt ttaaaacaat gggtgcgtgg gctgttttat gcttcgccgc tgacgacact
     6061 gacgcaagaa tggcaatagg acaagagatg gagatggacc cgacaaacga tgaattcata
     6121 atttatggcg ctccatctaa ttacttactt gatacctgca acatttacaa caaggctgcc
     6181 tgatggtggc ctttattttt ggcataaaca acagaataaa cacagcactg tgtattcatt
     6241 ccaacgagtg aatacacgga gcaatgtcgc tcgtaactaa acaggagccg acttgttctg
     6301 attattggaa atcttctttg ccctccaatg tgagggcgat tttttatctg tgaggatatg
     6361 aacagatgtc aaacatcaaa aaatacatca ttgattacga ctggaaagca tcaatagaaa
     6421 ttgaaatcga ccatgacgta atgacagagg aaaaacttca ccagattaat aatttctggt
     6481 cagactctga ataccgactc aataaacacg gctctttatt aaatgctgta ttaatcatgc
     6541 tggcgcaaca tgctctgctt atagcaattt caagcgactt aaatgcatat ggtgttgtgt
     6601 gtgagttcga ctggaatgat ggaaatggtc aggaaggatg gcctccaatg gatggtagcg
     6661 aaggaataag aattaccgat atcgatacat caggaatatt tgattcagat gatatgacta
     6721 tcaaagccgc ctgagcgcgg cgttaccgca taccaataac gcttcactcg aggcgttttt
     6781 cgttatgtat aaataaggag cacaccatgc aatatgccat tgcagggtgg cctgttgctg
     6841 gctgcccttc cgaatcttta cttgaacgaa tcacccgtaa attacgtgac ggatggaaac
     6901 gccttatcga catacttaat cagccaggag tcccaaaaaa tggatcaaac acttatggct
     6961 atccagacta aattcactat cgccactttt attggcgatg aaaagatgtt tcgtgaggcc
     7021 gtcgacgctt ataaaaaatg gatattaata ctgaaactga gatcaagcaa aagcattcac
     7081 taaccccctt tcctgttttc ctaatcagcc cggcatttcg cgggcgatat tttcacagct
     7141 atttcaggag ttcagccatg aacgcttatt acattcagga tcgtcttgag gctcagagct
     7201 ggacgcgtca ctaccagcag atcgcccgtg aagagaaaga ggcagaactg gcagacgaca
     7261 tgggaaaagg tctgccccag cacctgtttg aatcactctg catcgatcat ttgcaacgcc
     7321 acggggccag caaaaaagcc attacccgtg cgtttgatga cgatgttgag tttcaggagc
     7381 gcatggcaga acacatccgg tacatggttg aaaccattgc tcaccaccag gttgatattg
     7441 attcagaggt ataaagcgga tgagtacagc actcgcaacg ctggcaggga agctggctga
     7501 acgtgtcggc atggattctg tcgacccaca ggaactgatc accactcttc gccagacggc
     7561 atttaaaggt gatgccagcg atgcgcagtt catcgcattg ttgatcgtcg ccaaccagta
     7621 cggccttaat ccgtggacga aagaaattta cgccttccct gataagcaga acggcatcgt
     7681 tccggtggtg ggcgttgatg gctggtcccg catcatcaat gaaaaccagc agtttgatgg
     7741 catggacttt gagcaggaca atgaatcctg tacatgccgg atttaccgca aagaccgtaa
     7801 tcatccgatc tgcgttaccg agtggatgga tgaatgccgc cgcgaaccat tcaaaacccg
     7861 cgaaggcaga gaaatcaccg gcccgtggca gtcgcatccc aaacggatgt tacggcataa
     7921 agccatgatt cagtgtgccc gtctggcctt cggatttgct ggtatctatg acaaggatga
     7981 agccgagcgc attgtcgaaa atactgcata cactgcagaa cgccagccgg aacgcgacat
     8041 cactccggtt aacgatgaaa ccatgcagga gattaacact ctgctgatcg ccctggataa
     8101 aacatgggat gacgacttat tgccgctctg ttcccagata tttcgccgcg acattcgcgc
     8161 atcgtcagaa ctgacacagg ccgaagcagt gaaagttctt ggattcctga aacagaaagc
     8221 ctctgagcag aaggtggctg catgacaccg gacattatcc tgcagcgtac cggaatcgac
     8281 gtgagagctg tcgaacaggg ggatgatgca tggcacaaat tacggctcgg cgtcatcacc
     8341 gcttcagaag ttcacaacgt gatagcaaag ccccgctcag gaaagaagtg gcctgacatg
     8401 aaaatgtcct acttccacac cctgcttgcc gaggtttgca ccggtgtggc tccggaagtt
     8461 aacgctaaag cactggcctg gggaaaacag tacgagaacg acgccagaac cctgtttgag
     8521 ttcacttccg gcgtgaatgt tattgaatcc ccgatcatct atcgcgacga aagtatgcgt
     8581 accgcctgct ctcccgatgg tttatgcagt gacggcaatg gccttgagct gaaatgcccg
     8641 tttacctccc gggatttcat gaagttccgg ctcggtggtt tcgaggccat aaagtcggct
     8701 tacatggccc aggtgcagta cagcatgtgg gtgacacgaa aagatgcctg gtactttgcc
     8761 aactatgacc cacgtatgaa gcgtgaagga ctgcattatg tcgtggttga gcgggatgaa
     8821 aagtacatgg cgagttttga cgagatggtg ccggagttca tcgaaaaaat ggacgaggca
     8881 ctggctgaaa ttggttttgt atttggggag caatggcgat gaagcatcct cacgataata
     8941 tccgggtagg tgcgatcact ttcgtctact ccgttacaaa gcgaggctgg gtatttcccg
     9001 gcctttctgt tatcagaaat cccctgaaag cacagcggct ggctgaggag ataaataata
     9061 aacggggagc tgtatgcaca aagcatctcc cgttgagtta agaacgagta tcgagatggc
     9121 acatagcctc gctcaaattg gagtcaggtt tgtgccaata ccagtagaaa cagacgaaga
     9181 atttcatacg ttagcc
//
LOCUS       DAZKIP010000151         9192 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZKIP010000151 DAZKIP010000000
VERSION     DAZKIP010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.152"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..980)
                     /locus_tag="ACHOUK_004101"
     rRNA            complement(<1..980)
                     /locus_tag="ACHOUK_004101"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(1357..2214)
                     /gene="murI"
                     /locus_tag="ACHOUK_004102"
     CDS             complement(1357..2214)
                     /gene="murI"
                     /locus_tag="ACHOUK_004102"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFP9899444.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
     gene            complement(2159..4003)
                     /gene="btuB"
                     /locus_tag="ACHOUK_004103"
     CDS             complement(2159..4003)
                     /gene="btuB"
                     /locus_tag="ACHOUK_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFP9899445.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGGSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQPDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLVDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNIIIGHGNVGAG
                     VDWQKQSTAPGTAYVKDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     regulatory      complement(4068..4258)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            4372..5472
                     /gene="trmA"
                     /locus_tag="ACHOUK_004104"
     CDS             4372..5472
                     /gene="trmA"
                     /locus_tag="ACHOUK_004104"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFP9899446.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     gene            complement(5512..5871)
                     /gene="yijD"
                     /locus_tag="ACHOUK_004105"
     CDS             complement(5512..5871)
                     /gene="yijD"
                     /locus_tag="ACHOUK_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFP9899447.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(5871..6521)
                     /gene="fabR"
                     /locus_tag="ACHOUK_004106"
     CDS             complement(5871..6521)
                     /gene="fabR"
                     /locus_tag="ACHOUK_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFP9899448.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            complement(6625..7941)
                     /locus_tag="ACHOUK_004107"
     CDS             complement(6625..7941)
                     /locus_tag="ACHOUK_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate-proton symporter"
                     /protein_id="HFP9899449.1"
                     /translation="MTAIVNETVSVKKKPSGRRVIFASAFGNALEFFDFGVYNFFVVY
                     ISTLFFPPSADKNVALLLAFATFGVSFFMRPLGGIIVGAWADRFGRKPAMVFTIALMS
                     LGTLMIGIAPTYETAGYWGTATLVLARLIQGVAAGGEVGASMSLLVESAPANRRGFYS
                     SWSLATQGLATTFGGVVALGLSAWLPFATGSETVMAEWGWRVPFFIGVLLAPVGCWLR
                     LSLENDVPEPAHNKKAAASESAFSLLMQHKATIANGILLAIGSTVATYISLFYYGTWA
                     AKYLGMNQNYSHAAMLLAGVITFVGALLVGMLCDSVGRKKLILISRVMVLICSWPSFW
                     LLVNYPSPGMLLTVVFVMVSFTTLGGVPVMLLISELLPKRIRALGFALVYSIGVAIFG
                     GFAQYFATQSIVLLDSLTAPAWYLGGGTLLSMLALLYVKEPAKELQ"
     gene            complement(7984..9150)
                     /locus_tag="ACHOUK_004108"
     CDS             complement(7984..9150)
                     /locus_tag="ACHOUK_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HFP9899450.1"
                     /translation="MIEHYIRGFEEELREIRHQIHENPELGLQEFKTSALVAEKLRQW
                     GYEVEQGLATTGIVATLKVGDGEKSIGLRADMDALPIYENSGKPWASKHPGLMHACGH
                     DGHTTILLGAARYFAETRRFNGTLRLIFQPAEEMINGGEIMVKEGLFDHFPCDVIFGM
                     HNMPGLPVGKFYFQPGALMASMDQFHITVRGCGGHGAIPHKAIDPVLVAAHITTALQS
                     IVSRNVDPLEAAVITVGSIVAGEAANVIPDSAEMKISVRSLSRDTRQLLLTRIPALAQ
                     AQAASFGATAEVTHVNGTPVLVNDEEMARFAWQVACKTFGEDRAEFGIKPLMGSEDFS
                     FMLEAQPKGGFLLFGNGDVGEGSCMVHNPGYDFNDASLVPASSYWGALVEAWLQ"
BASE COUNT         2337 a         2541 c         2160 g         2154 t
ORIGIN      
        1 ggttcttcgc gttgcatcga attaaaccac atgctccacc gcttgtgcgg gcccccgtca
       61 attcatttga gttttaacct tgcggccgta ctccccaggc ggtcgactta acgcgttagc
      121 tccggaagcc acgcctcaag ggcacaacct ccaagtcgac atcgtttacg gcgtggacta
      181 ccagggtatc taatcctgtt tgctccccac gctttcgcac ctgagcgtca gtcttcgtcc
      241 agggggccgc cttcgccacc ggtattcctc cagatctcta cgcatttcac cgctacacct
      301 ggaattctac ccccctctac gagactcaag cttgccagta tcagatgcag ttcccaggtt
      361 gagcccgggg atttcacatc tgacttaaca aaccgcctgc gtgcgcttta cgcccagtaa
      421 ttccgattaa cgcttgcacc ctccgtatta ccgcggctgc tggcacggag ttagccggtg
      481 cttcttctgc gggtaacgtc aatgagcaaa ggtattaact ttactccctt cctccccgct
      541 gaaagtactt tacaacccga aggccttctt catacacgcg gcatggctgc atcaggcttg
      601 cgcccattgt gcaatattcc ccactgctgc ctcccgtagg agtctggacc gtgtctcagt
      661 tccagtgtgg ctggtcatcc tctcagacca gctagggatc gtcgcctagg tgagccgtta
      721 ccccacctac tagctaatcc catctgggca catccgatgg caagaggccc gaaggtcccc
      781 ctctttggtc ttgcgacgtt atgcggtatt agctaccgtt tccagtagtt atccccctcc
      841 atcaggcagt ttcccagaca ttactcaccc gtccgccact cgtcagcaaa gaagcaagct
      901 tcttcctgtt accgttcgac ttgcatgtgt taggcctgcc gccagcgttc aatctgagcc
      961 atgatcaaac tcttcaattt aaaagtttga tgctcaaaga attaaacttc gtaatgaatt
     1021 acgtgttcac tcttgagact tggtattcat ttttcgtctt gcgacgttaa gaatccgtat
     1081 cttcgagtgc ccacacagat tgtctgataa attgttaaag agcagttgcg acgcggcttt
     1141 cagctcactg tcgcgaggtg gcgtatatta cgctttcccg ctacagagtc aagcatttat
     1201 ttttgctttc tctgtcggaa ttctcaggag aaccccgctg acccggcggc gtgtttgccg
     1261 ttgttccgtg tcagtggtgg cgcattatag ggagttattc cggcctgaca agaggaaatt
     1321 taaaataatt ttccgaccgc gcaacattca accaaatcag cctaaaactg ccagtttttc
     1381 gagcgtttcg aagccgtaac gctgtaaaac gggcaataat tgttcagctt ctggcgtcat
     1441 tgccatacaa aaggcaatat tcgcatcggc agattttgca tccggggctt catgttctaa
     1501 caaccaggcc gttcggcgag caatcgctgc gccagaatcc accagccggg ttccctctgg
     1561 cagcacttgt aacagttctt cttgtagtag agggaaatgg gtgcaaccca atacaacggt
     1621 atctggcggc tctttcattc ttaaccacgg gcgtaggata cgttttagtg catccagaga
     1681 aacatcttcg ccatgcagct tcgcttcagc caactcaacc atctctgccg agcccagcat
     1741 ttctatctgg cattcattag cgaaacgcgc gatcagctca tgagtataag aacgtttaac
     1801 tgttccgcgg gttgccagta atccgacaat gccatttgcc gtcagacgtg cagcaggttt
     1861 aatcgccggc acgacaccaa caaccgggaa gtcgaacttt tcgcgtaatg caggaagtga
     1921 aacggtactg gcagtgttgc aagcgaccac agccagcgca aggggataac gttcttgcac
     1981 cgcggtgaca attgccacca ctcgctcaac aataaacgct tcgctttttt cgccatacgg
     2041 gaaagcgacg ttatcgaaag cataaatgta atggagatcc ggtaagagat gccggatctc
     2101 gtcatagacc gacaacccac cgacgccgga gtcaaacacc agcacggtgg gacgtggttc
     2161 agaaggtgta gctgccagac aaggtgtatt cccgtcctgc agtttggtag ccatagactg
     2221 tctcataatc tttgtcgaac aggttggcta ttttaccacg aactgtcagg tgagaggtga
     2281 ccggatacgc aaccgcaaga tcccacaagc tcacaccgcc cattttaacg gtttgataag
     2341 gataagatga gtaatcctta tcatagcgag tgcctaaata ctgataagta ataccccagt
     2401 cgaagtcata caactgccag tcgagctggt atttcacctg ctgtttagca cggcgtaaca
     2461 acggcgtgtc ggtaattgca ttgcgcgcat cgacataatc ataactcaca gtatgcgtca
     2521 gtggtccggt atcaaaattg gcggtcgcct cgacgccctt aatccgcgct ttcccttcgt
     2581 tgtaatattt cagggtgtga tcatcataat cgatcaagtc actgacatcg ttacgatatc
     2641 cggaaatacg ccagttcacc ccagcggtta agccttcaaa cgcgccttcc cactgtttgc
     2701 ttttctccgg gtccagattc ggatttccgt agaagccata cagttgcccc agatttggtg
     2761 ccttataaga tgtcccgtag gaagcaatga agcgataacc ttcgatgaat tcccaaccgg
     2821 cgctggtttg ccaggttcca tgacgaccaa actgtgagtt atcgtcgctg cgtgctgcgc
     2881 cttcaaaggt aaaatcgccg acttgttgca gcccggtcag atagatgccg gtattacgtt
     2941 gatcatatcc atccttaaca taagctgtgc ccggtgccgt gctctgcttc tgccagtcaa
     3001 cacccgcacc aacattaccg tggccaatga tgatgttgtt tgcccactgg acggtgtatt
     3061 gcttcatctc atcgagcgtc gccgacgaat cataacgacc ataatgggga tcgtagttgt
     3121 aatctttgct atggctatag ctggtaatga gttgtgattt aatcagttcg ccgttatatc
     3181 gcagcccggc gtcccaactt tgactataga gtttacgggt atcgaccaat ggtgaacccg
     3241 gagaataata cgcgtcataa ttggtacggt tatcatagcc atagccgcgc acaaagccgc
     3301 tccaggcatc agtaaagtta tgctccagcg cgccataaag cgttttactt aaaaaaccat
     3361 cgttatctgg ctgcgcttgc gttccggtat taccataggc aacaacatca taaccatgag
     3421 tatgggcata atcgcccaac aacgttactc gtgtcttatc ccccagttgt tgctgtgtag
     3481 agacatcata gttttgataa ctattgcttc cccaccctgc tgaaatttcc gttccgggtt
     3541 catcgcgcgt cgtgatgata ttcaccaccc cacctattgc atcggaacca taaacggcgg
     3601 agcgtggccc acggatatat tcaacacgct ggacaagcgc aatagggaac tggctaaggt
     3661 cggcagaacc actccccccc gccagattca ggcgtacgcc atcaattaac accaacacat
     3721 gactggcatt tgtaccgcga ataaaaatag atgagagctg acctgaaccg ccgttttggg
     3781 tgatatcgac gcccggaaga cggcgcagca catcattaac cgaggtcgac tgccagcggt
     3841 cgatatcctg acgcgtcaca acggtggttg gtgcaagcac agtgctgcgc ggctgttcaa
     3901 aacggttagc agtaacgacg agagtatccg ggctggtatc ctgtgcccaa gcggaaaagg
     3961 ctgtgacaga acacgccgtc agcagcgaag cttttttaat cattgtaaag catccacaat
     4021 agaagaagga tgccgcaggt ttcatcaata ttacgcgatg atgagaacca gatgcgacgt
     4081 tggccggcag gtcttcgggc ttggaggggt atctaagata ctaagagatg atgacttccc
     4141 accgaatggc agtgtccgca taacgcaatc atcgcacctt tccttaccgc tgcgcgtcag
     4201 ctccagattc gcactggatt ccctattaac tcacaggacc ggcaagtgga tgctacaggt
     4261 tgtaacaagt tactgtccag acgtagctca caaataggaa ttcatcaaga tctggacatc
     4321 tgatgagcaa tccctacaat cgccgcgtac tttaattttt caggatacat catgaccccc
     4381 gaacaccttc caacagaaca gtatgaagcg cagttagccg aaaaagtggt acgtttgcaa
     4441 agtatgatgg caccgttttc tgacctggtt ccggaagtgt ttcgctcgcc ggtcagtcat
     4501 taccggatgc gtgcggagtt ccgcatctgg cacgatggcg atgacctgta ccacatcatt
     4561 ttcgatcaac aaaccaaaag ccgtatccgg gtggatagct tccccgccgc cagtgaactc
     4621 atcaaccagt tgatgacggc gatgattgcg ggtgtgcgta ataatcccgt tctgcgccac
     4681 aagttgttcc agattgatta cctcaccacg ctgagtaatc aggcggtggt ttccctgctg
     4741 taccataaaa agctggatga tgagtggcgt caggaagcgg aagccctgcg cgatgcactg
     4801 cgcgcgcaga atctgaatgt gcatctgatt ggtcgggcaa cgaaaaccaa aatcgcgctg
     4861 gatcaagatt acatcgacga gcgtctgccg gtcgcaggga aagagatgat ctatcgtcag
     4921 gtagaaaaca gctttaccca gccgaacgcg gcgatgaata ttcagatgct ggaatgggcg
     4981 ctggacgtaa ccaaaggctc aaaaggcgat ttactggagc tgtactgcgg caacggtaac
     5041 ttctcattag cgctggcgcg caattttgat cgggtattag ccaccgaaat cgccaagcca
     5101 tcagtcgctg ccgcgcaata caacattgcc gctaaccata ttgataacgt acaaattatt
     5161 cgtatggcgg cagaagaatt tactcaggcg atgaatggcg tgcgcgagtt taaccgcctg
     5221 caagggatcg acttaaagag ttatcagtgc gaaaccattt ttgtcgaccc acctcgcagc
     5281 ggtctggaca gtgaaaccga gaaaatggtg caggcgtatc cacgtattct gtatatctcc
     5341 tgcaatccgg aaacgttatg caaaaatctg gaaacattaa gccagacgca caaggtcgaa
     5401 cgtctggctc tgtttgatca gttcccctac acgcaccata tggagtgcgg cgtattactg
     5461 accgcgaagt aaaacccatg ccggatgcgc cagcatccgg catgataccg attactcagc
     5521 aacttcctgc ttacggttac gcatcttcgc gccaatccag aacaccataa tgacggagag
     5581 tactgccggg aagaagttag agccgatatc cggatattcc gcacgaacta ccgtgctgta
     5641 cagcagtacg ccgagaataa aacaggcagc tgccagaccc ggcaagccta ccggcatggt
     5701 gcgattaaga taacgttgat gcaggcagta aaccgtcagc accagggaaa taatcgggaa
     5761 tacagaaaat ggcacaatgg agctaaacag cgctgcgaaa gtaccattaa tcgataagcc
     5821 agcaaccaac gccagcagca gcgtacctct atcttgattt gcttgtttca ttactcgtcc
     5881 ttcacatttc ccggaataat tgcggttttc tcttgttcac ggcgatacca gtaataagcc
     5941 cctttcgaaa tcattcgcag ttgcagtacc agtcgctctt ctaattgccg acgttgttca
     6001 acgccgacgt ccaacgcctc ggcacccgca ctgaagacaa ttgtcaccat tgcttcggct
     6061 tgcgcttcag taaacgcacg cggcatatgg ttttcgagtt ccagatagtc cgcaagttcc
     6121 gcaatgaagt gctgaatttc acgcgcaacg gcggcacgaa acgcagcgga ggtgccggag
     6181 cgttcccgca ataataaccg gaaggcgtta ggattattac cgatgaactc cataaatgtg
     6241 gagaccgagg tgcggatcac actcccgcct ttggcgatac gctgacgcgc ctggcgcatg
     6301 agttggcgta gcattaaacc gctctcatca accatggtca gacccagttc gtctacgtct
     6361 cggaaatgcc gataaaaaga ggtgggagca atgcccgctt cacgcgccac ttcacgcaaa
     6421 ctcaggctgg cgaagctgcg ttcagcactt aattggctaa atgcggcttc caccagcgaa
     6481 cggcgggttt tttctttttg ttgcgctctt acgcccatca cgatgtctga atccttgcca
     6541 aaagtactgc tggcactata ccagagaatg aacatactgg attgctccag tattgtgaat
     6601 gaacggtaac gcaataaaac atatttactg caattctttg gccggttctt ttacgtacaa
     6661 caacgccaac atcgacagca acgtgcctcc acccagatac cacgccggag ccgtcaaact
     6721 atccagcaac acgatggatt gcgtagcgaa atattgagca aaaccgccaa agattgccac
     6781 accaatgcta taaaccagcg caaaacccaa tgcccgaatc cgtttcggca acagttcaga
     6841 gatcaacaac atcactggca caccgccaag cgtggtaaag ctgaccatca cgaaaaccac
     6901 cgtcagcaac atgcctgggc ttgggtaatt caccaacagc cagaatgacg gccagctaca
     6961 gatcagcacc atcacacggg agattaaaat cagcttttta cgcccgaccg aatcgcacaa
     7021 catccccacc agcagcgcac ccacaaacgt aataacgccc gccagtaaca tcgctgcatg
     7081 agaatagttt tggttcattc ctaaatactt cgccgcccat gtaccgtaat aaaagaggga
     7141 gatataggtt gcgactgtac taccaatagc gagcaagata ccgttagcga tagtcgcttt
     7201 atgttgcata agtaaggaga aggcgctttc gctggctgcg gccttcttat tatgtgctgg
     7261 ctcaggaaca tcattttcca gactcaaacg caaccaacag ccgacaggcg ctaataacac
     7321 gccaataaaa aacggcacac gccagcccca ttcagccatg acggtttctg aaccggtcgc
     7381 aaagggcagc catgcactta aacctaaagc gacaacacct ccaaaagttg ttgccaggcc
     7441 ttgagtcgcc agtgaccaac tgctgtaaaa accgcgacga tttgccggag ctgattcaac
     7501 caacaaagac attgaggcac cgacttcgcc tcctgccgca acgccctgaa ttagacgcgc
     7561 tagcaccagc gtcgcggttc cccaatagcc cgccgtttca tacgtcggtg cgatgccgat
     7621 catcagtgta ccaagactca tcagcgcaat ggtgaacacc atcgcgggtt tgcgtccgaa
     7681 acgatcggcc caggccccca caataatgcc gccgagcggg cgcataaaaa agctcacgcc
     7741 gaaagtggca aacgccagca gtagcgcgac gtttttatct gcactgggcg gaaaaaatag
     7801 cgtgctgata tagaccacaa aaaagttgta aacgccaaaa tcaaaaaatt cgagcgcatt
     7861 gccaaacgcc gaggcaaaaa tcacccgcct gccgctgggc tttttcttca cactcacggt
     7921 ttcatttacg atcgccgtca ttccatgttc cttgccaaaa agtgttgttg tgtgttgttt
     7981 tatttattgc agccaggctt ctaccagtgc gccccagtag ctgcttgccg ggaccagact
     8041 tgcatcgttg aagtcgtaac ccgggttatg caccatgcag gaaccttccc cgacatcgcc
     8101 attaccgaat aacaggaaac cgcctttggg ttgggcttcc agcatgaaag agaaatcttc
     8161 actgcccatc aggggcttga tgccaaactc ggcccgatct tcaccaaacg ttttacacgc
     8221 cacctgccag gcgaagcgcg ccatctcctc gtcatttact aatactggtg taccgttaac
     8281 atgcgtaacc tcagccgtag cgccaaagct ggcagcctgg gcttgtgcca gagcaggaat
     8341 acgggtcaga agaagttgtc gggtatcgcg gctaagagag cggacgctga ttttcatctc
     8401 agcactgtca ggaatgacgt tagccgcctc acccgcaaca atgctgccga cggtaattac
     8461 cgccgcttcc agcggatcga cattgcgtga cacaatgctt tgtaatgcgg tggtgatatg
     8521 tgcggcaact agcaccggat cgatggcttt gtgcgggatc gcaccgtgtc cgccacaacc
     8581 acgaactgta atatggaact gatccattga cgccatcagt gcccctggct ggaaataaaa
     8641 cttacccacc ggcagaccgg gcatgttatg catgccgaaa atgacatcgc aggggaaatg
     8701 atcaaaaagc ccctctttaa ccatgatctc accgccatta atcatctctt ccgcaggctg
     8761 gaagataaga cgtaacgtgc cgttaaaacg gcgggtttcg gcaaaataac gcgcggcccc
     8821 cagcagtatg gtggtatgcc cgtcatgccc gcaggcgtgc attaaaccag gatgtttact
     8881 ggcccagggc ttgccactat tttcataaat cggcagcgca tccatatccg cccgcaggcc
     8941 aatacttttc tctccgtcgc ccacttttaa cgtggccact atccccgtcg tcgccagtcc
     9001 ttgctctact tcatagcccc actgacgcag cttttccgca accaaagcac tggttttaaa
     9061 ttcttgtaat cccagctccg ggttttcgtg gatctgatgg cggatctcac gcagttcttc
     9121 ctcaaaccct cgaatatagt gttcgatcat cggctattcc tttattcact tataaacagg
     9181 ctaattaacg ga
//
LOCUS       DAZKIP010000152         9173 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZKIP010000152 DAZKIP010000000
VERSION     DAZKIP010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9173
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.153"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..246
                     /locus_tag="ACHOUK_004109"
     CDS             <1..246
                     /locus_tag="ACHOUK_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFP9899451.1"
                     /translation="EVSKGKLIDTGFCIFALSKLAMALSSTLDSIPLSMQRQFPDLTP
                     RHLDHLKTLIAKGANQCARAGDKLPDLLDEYIRATTE"
     gene            218..2147
                     /locus_tag="ACHOUK_004110"
                     /pseudo
     CDS             218..2147
                     /locus_tag="ACHOUK_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
     gene            2131..2337
                     /locus_tag="ACHOUK_004111"
     CDS             2131..2337
                     /locus_tag="ACHOUK_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFP9899452.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDAESVLGTTRRRRRPLGVRL"
     gene            2334..3926
                     /locus_tag="ACHOUK_004112"
     CDS             2334..3926
                     /locus_tag="ACHOUK_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9899453.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMSGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTETTQ
                     LFRTRFKAVSPKRVDTPGHGMGNRFLRAGVEVDRYGRAVAYHICEDDFPRSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYA
                     ATIESDLDTEKAFEYIAGAPQGQKDNPLINILEKFSSWYDTNNVTLGGVKIPHLFPGD
                     DLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRY
                     FMGRRKFIAARLATQMFSYWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMA
                     IDGLKEVQESVMRIEAGLSTYEKEPALMGEDYQDIFRQQVRESAERQKAGLSRPVWIA
                     QAYQQQIAESRRPEEETTPRET"
     gene            3916..5421
                     /locus_tag="ACHOUK_004113"
     CDS             3916..5421
                     /locus_tag="ACHOUK_004113"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFP9899454.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADSQVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYSGAHKVDGN
                     QFEALPAEVRQNMQQRIDAARRMFAEKVAMFTGLSVDAVTGTEAAVFEGQSGIDAGLA
                     DELVNASDAISVMATALNSNVRGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAAAAPQQPVASTQSEADRIMACEEANGREQLAATLAAMPE
                     MTVEKARPILAASPQADAGPSLRDQIMALDEAKGAEAQAEQLAACPGMTVESARAVLA
                     AGSGKAEPVSASTTALFERIMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5458..5805
                     /locus_tag="ACHOUK_004114"
     CDS             5458..5805
                     /locus_tag="ACHOUK_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFP9899455.1"
                     /translation="MVTKNITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EASGKLVVWDGQKAGSAVGILVLPLEGTETVLTYYKSGTFATEAIRWPESVDEHKKAN
                     AFAGSALSHAALP"
     gene            5863..6114
                     /locus_tag="ACHOUK_004115"
                     /pseudo
     CDS             5863..6114
                     /locus_tag="ACHOUK_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
     gene            6111..6851
                     /locus_tag="ACHOUK_004116"
     CDS             6111..6851
                     /locus_tag="ACHOUK_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9899456.1"
                     /translation="MPLEPVKGAGTTLWVYNGQGDAYANPLSDDDWQRLAKVKDLTPG
                     EMTAESYDDNYLDDEDADWTATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFESGDVR
                     AYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSKITPVSAI
                     KVTPTSGTVAKGKTTTLTVSFEPESATDKTFRAVSADPSKATISVKDMTITVNGVATG
                     KVQIPVVSGNGQFAAVAEVTVTEAGAAG"
     gene            6865..7296
                     /locus_tag="ACHOUK_004117"
     CDS             6865..7296
                     /locus_tag="ACHOUK_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFP9899457.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAESSGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTQSPSMNEAVMKIEQEVLTTWPADAIARAED
                     VVLCLSGMSGAVRPDTDITEVAKNNTLTDDDFSAGKSSTAS"
     gene            7323..7736
                     /locus_tag="ACHOUK_004118"
     CDS             7323..7736
                     /locus_tag="ACHOUK_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFP9899458.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWHRFYRTHYFQDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLVPRHEEEQVERPDEDKMLMQKAAGLAGGVRFGGDGGRD
                     ILSSADVADVMVDDAALMMASAGIPGGVRYVPAGW"
     gene            7717..>9173
                     /locus_tag="ACHOUK_004119"
     CDS             7717..>9173
                     /locus_tag="ACHOUK_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFP9899459.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLDTDVRKTASAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGVTSLAVATGALVYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTADRMLTLSRAGQAAGLTFNQARESLAALVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLIAMVRQFRNVTAEQIAYVAQLQRSGDEAGALQAANDIA
                     TKGFDEQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPESSADMLASAQKAFDE
                     ADKKWQWYQSRSQRRGKTASFRANLQGAWNDRENARLGLAAATLQSDMEKAGELAARD
                     RAERDASQLKYTGEAQKAYERLLTPLEKYTARQEELNKALKDGKILRADYNTLMAAAK
                     KDYESTLKKPKSSGVKVSAGERQEDQAHAALLALET"
BASE COUNT         2099 a         2244 c         2901 g         1929 t
ORIGIN      
        1 gaggtgtcaa aaggaaaact gattgacacc gggttctgta tttttgccct cagtaagctg
       61 gcaatggcgt tatccagtac gcttgattcc atccctttat ccatgcagcg acagtttcct
      121 gatttaacac cgcgccatct tgaccatctg aaaaccctta ttgctaaggg ggcaaatcag
      181 tgtgcgcggg caggggataa attaccggat ttacttgatg aatatatcag agcaacaact
      241 gaataatatg atgagcgctg tcacaactgc attacagccc ctgataaggg cattgccggt
      301 gacgccagtt gaatgggctg atcaaaatta ttatctgcct aaagaatctt catatggtga
      361 gggcgaatgg aaaacgctgc cattccagat cgccatcatg aacagtatgg ggaatgatca
      421 gatccgcact gttaatctga ttaaatctgc ccgtgttggc tatacaaaga tgttgctggg
      481 gggggggcgg gtattttatt gagcataaat cccgaaacag tctgcttttt cagcccacgg
      541 attctgccgc tgaagatttt atgaagtctc acgtggaggc gacgattcgg aacgtgccat
      601 gcctgaaaga cctttcccca tggctgggtc gtaaacatcg tgacaatact ctcacgctga
      661 aacgcttttc atcgggcgtc ggtttctggt gcctgggcgg cgctgccgcc aaaaactacc
      721 gtgaaaaatc cgttgacgtg gtctgctatg acgaactttc ctcgttcgag ccggatgtcg
      781 aaaaagaggg ctcgccaacc ctgctggggg ataagcgtat tgaggggtcg gtgtggccaa
      841 aatccattcg cggctcgacg cctaaaatca aaggcacctg ccagatcgaa aaagccgcta
      901 acgagtcggc gcattttatg cgtttttatg tgccctgccc gcactgtggg gaggcgcagt
      961 atctgaaatt tggcgatgag tccacgcctt ttgggcttaa atgggagaag gacagccctg
     1021 aaagtgtttt ctacctctgt gaacatcatg gctgcgtgat ccatcagtct gaactggacc
     1081 agagcaacgg gcggtggatc tgtgaaaaca cgggcatgtg gacccgtgac ggtctgacgt
     1141 ttttcagcgc ccggggtgat gaaattccgc cgccgcgctc catcatgttc catatctgga
     1201 cggcgtacag tccgttcacc acctgggtac agattgtcta tgactggctg gatgcactga
     1261 aagatcccaa cggcctgaaa acctttgtga acaccacgct gggcgagacc tgggaagagg
     1321 ccgtgggcga aaaactcgat caccaggtac tgatggataa ggtggtgcgt tacacggcgg
     1381 cggtgcctgc ccgggtggtt tatctgacgg cgggcattga ctcgcagcga aaccgttttg
     1441 agatgtatgt ctggggatgg gctccgggag aggaagcctt tctggtggat aaaatcatca
     1501 ttatggggcg tcctgatgag gaagagacgc tgttacgtgt ggatgcggcg atcaacaaaa
     1561 aataccgcca tgcggatggc accgaaatga ctatttcccg tgtctgctgg gacaccgggg
     1621 ggatcgatgg tgaaattgtt tatcagagat caaaaaaaca cggtgttttc cgggtgctgc
     1681 cggtaaaagg cgcatctgtc tatggcaagc cggtgatcac catgccaaaa acccgcaatc
     1741 agcggggcgt gtatctgtgt gaagtgggaa cggacaccgc aaaagaaatt ctctatgccc
     1801 gtatgaaagc cgatccctcg cctgcggatg aagccacgtc gtatgccatc cgttttcctg
     1861 atgatccgga gattttttcg cagacagagg cgcagcaact ggtggcggaa gagctggtgg
     1921 agaagtggga aaaaggaaag atgcgtctgc tgtgggataa caaaaagcgg cgtaacgaag
     1981 cgctggactg cctggtgtat gcctacgcgg cattacgtgt gtccgtgcaa cgctggcagc
     2041 ttgatctggc tgtactggca aaatcccggg aagaagagac gacccggcca accctgaaag
     2101 aactggcagc gaagctgtcc ggaggagtga atggttacag tcgctgaact gcaggcgctg
     2161 cgtcaggcgc gccttgattt attaaccggt aaacgggtgg tgtctgtcca gaaagatggt
     2221 cgcagaattg aatatacggc ggcttctctg gatgagctta accgggcgat caatgatgcg
     2281 gagtcggtac tggggacaac ccggcgtcgc cgtcgtccgc tgggagtgag gttatgaaac
     2341 gaacgcctgt cctgattgat gtgaacggcg ttccgcttcg tgagagtctc agctacaacg
     2401 ggggcggtgc aggatttggc gggcaaatgg cggagtggtt gccaccggcg cagagtgccg
     2461 atgcggccct gctgcccgcg ttgcgtctgg ggaatgcccg ggcagatgat ctggtgcgca
     2521 ataacggaat agcggccaat gcggtggcac tgcataagga tcacattgtc gggcatatgt
     2581 ttcttatcag ctaccgtccg aactggcgct ggctggggat gcgggagacc gcagcaaaaa
     2641 gctttgtcga tgaggtggag gcggcctggt cggaatacgc cgaagggatg tctggcgaga
     2701 tcgacgtgga aggaaaacgc acgttcacgg aatttatccg tgaaggtgtg ggcgttcatg
     2761 cgtttaacgg cgaaatcttt gtgcagccgg tctgggatac ggaaaccacg cagttattcc
     2821 gtacgcgttt taaagccgtg agtccgaaac gggtggacac gccaggacac ggtatgggga
     2881 accgttttct gcgggccggg gtggaggtcg atcgatatgg ccgtgccgtt gcgtaccata
     2941 tctgtgagga tgattttcct cgctccggga gtggacgatg ggaacggatc ccgcgtgaac
     3001 ttcccaccgg gcgtccggcc atgctgcata ttttcgagcc ggtggaggac gggcagaccc
     3061 gtggggccaa ccagttttac agcgtcatgg aacggctgaa gatgctcgat tccctgcagg
     3121 caacacagct tcagtcggcc attgtgaaag ccatgtatgc agcgacgatt gaaagtgacc
     3181 ttgataccga aaaggccttt gaatatatcg ccggtgcgcc gcaggggcag aaggataatc
     3241 cgcttattaa tattctggag aagttctcca gctggtatga cacgaataac gtgacgctgg
     3301 gtggtgtcaa aattccgcac cttttccccg gggatgatct gaaactacag actgcgcagg
     3361 attcagacaa tggattttcg gcgcttgaac aggcgctgct gcggtatatc gccgccggtc
     3421 ttggcgtttc ctacgaacag ttgtcccgtg attactcgaa ggtcagttat tcaagtgcca
     3481 gggcctctgc caatgagtcg tggcgctatt ttatggggcg gcgaaaattt attgcggccc
     3541 ggctggccac gcagatgttt tcctactggc tggaagaggc acttcttcgg gggattatcc
     3601 gtccgccacg ggcgcgtttt gatttttatc aggcgcgatc agcctggtca cgggcagagt
     3661 ggattggtgc cggaagaatg gccattgacg ggctcaagga ggttcaggaa tcggtgatgc
     3721 gcattgaggc cggactgagc acgtatgaga aagagccggc gctgatgggc gaggattatc
     3781 aggacatttt ccgccagcag gtcagggaat ctgcagagcg gcaaaaagcc ggactctcac
     3841 gtccggtgtg gatagcgcag gcgtatcagc agcagatagc ggagagtcgc aggccggaag
     3901 aggagacaac accccgtgag acgtaatctt tcacacatta ttgccgcagc attcaatgaa
     3961 ccgctgcttc tggagcccgc ctatgcgcgg gttttctttt gcgcgctcgg gcgcgagatg
     4021 ggggcagcaa gtctttcggt accacagcag caggtacagc ttgatgctcc cggaatgctg
     4081 gctgaaacgg acgagtacat ggccggaggt aaacgaccgg cccgtgttta ccgggtggtg
     4141 aacggtattg ctgtactgcc ggtgaccggc acgctggtgc accggctggg gggtatgcgg
     4201 ccattttccg gaatgacagg ctatgacggc attgtcgcct gtcttcagca ggcaatggcg
     4261 gatagccagg tgcggggcgt actgctggac attgacagtc cgggcgggca ggccgccggc
     4321 gcgtttgact gcgctgacat gatttaccgc ctccgtcagc agaagccggt ctgggcactg
     4381 tgcaatgaca cggcctgttc tgcagccatg ctgctggcgt cggcctgctc ccgacggctg
     4441 gttacccaga catcccgtat cggctccatt ggcgtgatga tgagccatgt cagctatgcc
     4501 ggtcatctgg cgcaggccgg tgtggatatc acgctgattt actcaggggc gcacaaggtg
     4561 gatggcaatc agtttgaagc cttaccggca gaggttcgcc agaacatgca gcagcgcatt
     4621 gatgcggcgc gccggatgtt tgccgaaaaa gtggccatgt ttaccggtct gtctgttgat
     4681 gccgtcacgg gaacagaggc cgccgttttt gaaggtcagt ccggcattga tgccgggctg
     4741 gcggatgaat tagtcaatgc gtcggatgcc atcagtgtga tggccacggc gctgaacagt
     4801 aatgtcagag gaggcactat gccgcaatta actgcaacgg aagccgccgc gcaggagaac
     4861 cagcgagtga tggggatcct gacatgccag gaagcgaaag gacgtgaaca gcttgccacg
     4921 atgctggcag gacaacaggg catgagcgtt gaacaggccc gggcgattct ggccgcggcg
     4981 gcaccgcagc agccggtggc atccacgcag agtgaagccg atcgcattat ggcgtgtgaa
     5041 gaagcgaacg gtcgtgaaca actggcggca acgctggcgg cgatgccgga gatgacggtg
     5101 gaaaaagccc gcccgatcct ggctgcttca ccgcaggcgg atgccggacc ctcactccgt
     5161 gatcagatca tggcactgga tgaggcaaaa ggggctgagg cgcaggctga acagctggct
     5221 gcctgcccgg gaatgactgt ggagagcgcc cgggctgtgc tggctgcggg atcaggtaag
     5281 gcagaaccgg tctctgcatc cacaaccgcc ctgtttgaac gcatcatggc gaaccattca
     5341 ccggctgcgg tacagggtgg cgtgccacag acgtcagcag acggtgatgc ggacgtgaaa
     5401 atgctcatgg ccatgccatg aagtcagtgc tgaccatcaa caggaggttt ttacaatatg
     5461 gtaacgaaaa acatcactga acagcgtgcg gaagtacgta tttttgccgg taatgatccg
     5521 gctcataccg ccacaggcag cagcgggatt tcctcggcaa caccggcact gacgcccctg
     5581 atgctggatg aagccagcgg gaaactggtg gtctgggacg gacagaaagc cggtagtgca
     5641 gttggcatac tggtactgcc gcttgaaggc acagagacgg tactgaccta ttacaagtcg
     5701 gggacctttg cgacggaggc aatccgctgg cctgaaagtg tggatgaaca caaaaaggca
     5761 aatgcctttg ccggcagtgc cctgagtcac gcggcgctgc cgtaacacgt tatcaggcca
     5821 ccgcggtggc ctgactgatt tctgaatgaa aggaactgat ttatgggatt gtttacgacc
     5881 cgccagttac tcggttatac cgaacaaaaa gtgaaatttc gtgcgctgtt tctggagctg
     5941 tttttccgcc gtacggtgaa tttccatacc gaagaggtga tgctggacaa aattaccgga
     6001 aaaacgccgg tggcggccta tgtctccccg gttgttgaag gaaaagtgct gcgtcatcgc
     6061 ggtggtgaaa cccgcgtgtt acgtccgggc tacgtcaagc cgaaacacga attcccctgg
     6121 agccggtaaa aggagccggt accaccctgt gggtttataa cggtcagggt gacgcctatg
     6181 caaacccgtt gtcagacgat gactggcagc gactggctaa ggtgaaggat ctgacgccgg
     6241 gcgagatgac ggcagaatcc tacgatgata actacctgga tgatgaagac gcggactgga
     6301 ccgcgaccgg gcaggggcag aaatctgcag gtgataccag ttttacgctg gcctggaaac
     6361 cgggagagga aggccagaaa gggcttatag gctggtttga aagcggcgat gtccgggcct
     6421 ataaaatccg ttttccgaat ggcacggtgg atgtgtttcg tggctgggtc agcagtatcg
     6481 gtaaggccgt gacggcgaaa gaagtgatca cccgcacggt gaaagtcact aacgtgggta
     6541 aaccttctgt agcggaagaa cgcagcaaaa ttacgccggt cagtgcgatt aaggtgacgc
     6601 cgacatccgg tacggtggca aaagggaaaa caaccaccct gacggtttct tttgagccgg
     6661 aaagtgcaac agacaagacg ttcagagcgg tttccgccga tccgtcgaaa gccaccatta
     6721 gtgtgaaaga tatgacaatt acggtaaacg gcgtggcgac aggtaaggtg cagatccctg
     6781 tggtgagcgg aaatggtcag ttcgccgcag tggctgaagt caccgttact gaagcgggcg
     6841 ctgcagggta aacggaggtc atacatgttt ctgaaaacag aacaatttga atataacggt
     6901 gtgtctgtca cgctttccga attgtctgcg ctgcagcgta tcgagcatct tgccctcctg
     6961 aaacggcgtg cagaacaggc agaatccagc ggcaacctgc aggtaagcgt ggaagatctc
     7021 gtcagaaccg gcgcgtttct ggtggcgatg tccctgtggc ataaccatcc acagaaaacg
     7081 cagtcaccgt caatgaatga ggccgtgatg aagatagagc aggaagtgct caccacctgg
     7141 cctgccgatg ccattgcccg ggcggaagac gtggtgttgt gcctgtccgg gatgagcggg
     7201 gctgttcgtc cggatactga tattactgaa gtggcgaaaa ataacacgct gactgatgat
     7261 gatttttctg cgggaaagtc ttcgacggcg agctgaactt tgccctcaga ctggcgcgtg
     7321 agatggggag acccgactgg cgcgccatgc ttgccgggat gacatccacc gaatatgccg
     7381 actggcaccg tttttaccgc acgcattatt ttcaggatac ccagctggat atgcattttt
     7441 ccgggctgac gtacgctgta ctcagcctgt ttttttgcga tccggatatg catccctctg
     7501 atttcagtct gcttgtcccc cggcatgagg aagagcaggt ggagaggccg gatgaggaca
     7561 aaatgctgat gcagaaagcg gcaggacttg ccggaggcgt ccggttcggt ggggacggag
     7621 ggcgcgatat tttatcgtct gcggatgtgg cggatgtcat ggtggatgat gccgcattaa
     7681 tgatggcttc agcggggatt ccgggaggtg tgagatatgt cccagccggt tggtgatctt
     7741 gttattgacc tgagtctgga tgctgtccgt ttcgatgagc agatgagccg ggtaaggcgt
     7801 catttttcag gtctggatac cgacgtcaga aaaaccgcca gtgctgttga acagggcctg
     7861 agccgccagg cgctggctgc acaaaaagcc gggatttccg tcgggcagta taaagcggcc
     7921 atgcgaaccc tgcccgcaca gtttacggat atcgccacgc agcttgccgg tggtcagaat
     7981 ccctggctga tcctgctgca acagggcggt caggtgaagg actccttcgg cgggatgatc
     8041 cccatgttca gggggcttgc cggtgcgatc accctgccga tggtcggggt cacctcgctg
     8101 gcggtggcga caggtgcgct ggtgtacgcc tggtaccagg gagattccac gctttcagcg
     8161 tttaataaaa ccctggttct ttccggtaat cagtccggac tgactgccga tcgcatgctg
     8221 acgctctcaa gagccgggca ggcagcaggg ctgacgttta accaggcgag agagtcactg
     8281 gcagccctgg tgaatgccgg tgtgcgtggt ggtgaacagt ttgatgccat caaccagagt
     8341 gtcgcgcgtt ttgcttctgc atccggtgtg gaggtggaca aggttgcaga ggctttcgga
     8401 aaactgacca ccgaccctac gtcggggctg attgcgatgg tgcgccagtt ccgtaacgtg
     8461 acggcagagc agattgcgta tgttgcgcag ctgcagcgtt ccggtgatga ggccggggcc
     8521 ttacaggcgg cgaacgatat cgccacgaaa ggctttgatg agcagacccg tcgcctgaaa
     8581 gaaaacatgg ggacactgga gacctgggcg gataaaaccg ggaaggcatt caaatcgatg
     8641 tgggatgcca ttctggatat cggtcgtcct gagtcctcag cggatatgct cgccagtgca
     8701 cagaaggcat ttgatgaggc ggataaaaaa tggcagtggt accagagccg gagccagcgc
     8761 cggggaaaaa ccgcctcttt ccgggccaac cttcagggcg catggaatga ccgggaaaat
     8821 gcccgtctgg ggctggcagc ggccacgctg cagtcggata tggaaaaagc cggtgaactg
     8881 gccgccaggg accgggccga acgggacgca tcacagctga agtataccgg agaggcgcag
     8941 aaggcgtatg agcgtctgct gacgccgctg gagaaatata ccgcccgtca ggaagaactg
     9001 aataaggccc tgaaagacgg gaaaatcctg cgggcggatt acaacacgct gatggcggcg
     9061 gcgaaaaagg attatgaatc gacgctgaaa aagccgaagt cgtcaggagt caaagtgtca
     9121 gccggtgagc gtcaggaaga ccaggcgcat gctgccctgc tggcgcttga aac
//
LOCUS       DAZKIP010000153         9163 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZKIP010000153 DAZKIP010000000
VERSION     DAZKIP010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9163
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.154"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..920
                     /locus_tag="ACHOUK_004120"
     CDS             <1..920
                     /locus_tag="ACHOUK_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFP9899460.1"
                     /translation="RKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHKGIVNAAKVLNLT
                     PSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLESILGALDIEGSYD
                     KQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQLSQFQTDLIIDNM
                     FCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLLPEGQNFSGLRQR
                     IQEMFPDRQISFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWPLEKLPFPSLNEE
                     QIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            1129..2094
                     /gene="lipA"
                     /locus_tag="ACHOUK_004121"
     CDS             1129..2094
                     /gene="lipA"
                     /locus_tag="ACHOUK_004121"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFP9899461.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(2195..2398)
                     /gene="tatE"
                     /locus_tag="ACHOUK_004122"
     CDS             complement(2195..2398)
                     /gene="tatE"
                     /locus_tag="ACHOUK_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFP9899462.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(2527..3315)
                     /locus_tag="ACHOUK_004123"
     CDS             complement(2527..3315)
                     /locus_tag="ACHOUK_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFP9899463.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            3408..3791
                     /gene="crcB"
                     /locus_tag="ACHOUK_004124"
     CDS             3408..3791
                     /gene="crcB"
                     /locus_tag="ACHOUK_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFP9899464.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(3845..4054)
                     /gene="cspE"
                     /locus_tag="ACHOUK_004125"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(3845..4054)
                     /gene="cspE"
                     /locus_tag="ACHOUK_004125"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFP9899465.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(4229..4789)
                     /gene="pagP"
                     /locus_tag="ACHOUK_004126"
     CDS             complement(4229..4789)
                     /gene="pagP"
                     /locus_tag="ACHOUK_004126"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFP9899466.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIVQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            5378..6763
                     /gene="dcuC"
                     /locus_tag="ACHOUK_004127"
     CDS             5378..6763
                     /gene="dcuC"
                     /locus_tag="ACHOUK_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFP9899467.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(6804..7484)
                     /gene="dpiA"
                     /locus_tag="ACHOUK_004128"
                     /gene_synonym="citB"
     CDS             complement(6804..7484)
                     /gene="dpiA"
                     /locus_tag="ACHOUK_004128"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFP9899468.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(7453..9111)
                     /gene="dpiB"
                     /locus_tag="ACHOUK_004129"
     CDS             complement(7453..9111)
                     /gene="dpiB"
                     /locus_tag="ACHOUK_004129"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFP9899469.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISEVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
BASE COUNT         2333 a         2326 c         2072 g         2432 t
ORIGIN      
        1 gcagaaaatc gtcagaaggt aaaccacaaa tatttacaac cttacgaaat attgatctta
       61 atcttctgac tatttttgaa gctgtatatg tacataaagg gatcgttaat gcagcgaaag
      121 tgcttaatct gaccccctcg gcaatcagtc agtctattca gaaactgcgc gttatattcc
      181 ctgacccatt gtttattcgc aaaggccagg gtgtcactcc taccgcattt gcgatgcatc
      241 tacatgagta tatcagtcag ggccttgagt ccattcttgg cgcgctggat atcgaaggaa
      301 gctatgataa gcaacgaacg ataactattg cgaccacccc ctcggtcgga gctctggtcc
      361 ttcctgtcat ttatcgggcg attaaaactc actatccgca gcttttactg cgcaacccgc
      421 ccatcagcga cgcggaaaac caactcagtc agtttcaaac cgatctcatc atagataaca
      481 tgttttgcac caatcgtacg gtgcaacatc atgttctgtt caccgacaat atggtgttaa
      541 tttgccgcga gggaaatcca ctactctctt tagaagatga cagagagact atcgacaacg
      601 ctgcacatgt actcctgtta ccggaaggac aaaatttcag cggtctgcgg cagagaattc
      661 aagagatgtt tccggaccgg cagatcagct tcaccagcta caacattttg acaatcgctg
      721 cactggttgc caacagtgac atgttagcga ttattcccag ccgtttttat aacctgttta
      781 gccgttgctg gccgctggaa aaactgcctt ttccgtcctt aaatgaggag caaatagatt
      841 tctctatcca ctacaacaaa ttcagcctgc gtgatcccat cctgcacgga gtaatagatg
      901 ttatccgtaa tgcattttga aaaagtattg ttacagccag atcacagcgc agaacgataa
      961 aatggcacaa tccaacaaca attttacatt ttcgcgaccg ctttggctgc tttcaggtcc
     1021 gtttcaatga tatactgcca gtcgttaatt caaaaatagt tgataattac aacaatctat
     1081 tgaattgaaa cgctttcctt cgtaattcgc aactggaaca cgcacgctat gagtaaaccc
     1141 attgtgatgg aacgcggtgt taaataccgc gatgccgata agatggccct tatcccggtt
     1201 aaaaacgtgg caacagagcg cgaagccctg ctgcgcaagc cggaatggat gaaaatcaag
     1261 cttccagcgg actctacacg tatccagggc atcaaagccg caatgcgcaa aaatggcctg
     1321 cattctgtct gcgaggaagc ctcctgccct aacctggcgg aatgcttcaa ccacggcaca
     1381 gcaacgttta tgatcctggg cgctatttgt acccgccgtt gtccgttctg tgacgttgcc
     1441 cacggtcgcc cggtagctcc tgatgccaat gagccagtga aactggcgca gaccattgcc
     1501 gatatggcgc tgcgttatgt ggttatcacc tccgttgacc gtgatgacct gcgcgatggc
     1561 ggtgcccaac actttgcgga ttgtattact gccattcggg aaaaaagccc gcaaatcaaa
     1621 attgaaactc tggtgccgga tttccgcggt cgtatggatc gtgctctgga tattctgact
     1681 gcaacgcccc cagatgtgtt caaccataac ctggaaaacg taccgcgtat ttaccggcag
     1741 gtacggcctg gcgcagatta caactggtcg ctgaagctgc tggaacgctt taaagaagcg
     1801 catccggaaa tcccgaccaa gtctggtctg atggtgggtc tgggtgaaac caatgaagaa
     1861 attattgagg taatgcgcga cctgcgccgt catggtgtga cgatgttaac gctggggcaa
     1921 tatttgcagc caagccgcca tcacctgccg gttcaacgtt acgttagccc ggatgagttc
     1981 gacgaaatga aagccgaagc gctggcgatg ggctttaccc atgctgcatg cggtccgttt
     2041 gtccgctctt cttaccacgc cgatttgcag gcgaaaggga tggaagttaa gtaatcctgt
     2101 aataattact tacatctgtc tgataaaaaa cccgcttttt gaagcgggtt ttttgtatcg
     2161 aacaagatat tgagggagcg tcctgctcgc cacgtcactc tttatgagag agcttttcag
     2221 cctgaagatc aacgtctgcg ccttttttcg ccgcagcatc gtcatcattc atcgccttct
     2281 tgaacccttt aatggccgct ccaaggtctc cgcccagcgt acgtaacttc ttagtcccaa
     2341 acagcagaac gaccagcgcc gcaactacca gcagtttggt aatactaatc tcacccatag
     2401 ataccttctt gacatataac cggcaacaga acgcgcctta aaaaccaaag aatattaacg
     2461 gtcattcgac gcgcgcacac aatagcaatc tgtaacaagg tgaatcaagc gccgagtaaa
     2521 aaaacatcat aataattgcg gcggcgcaaa gcgacggttg tttaagacgg gcagttgcgc
     2581 gcgcacctga cgcacacgtt cgggcgtcac ttccgccata atgagtgcag gcatttctga
     2641 cgctgccgca atggtgacgc caaagggatc tataatccgg ctttgcccga tatttttgtt
     2701 cccgcactcc cccgccgcca ccatataaca ggtggtatcc agcgcacgag cggcaagcaa
     2761 cgttgaccag tggtgctctt tgagcggccc gcgaacccag gcggcaggaa gtaccaggat
     2821 ttcagctccc tgtaatgcct gtgccagcgc cagctctgga aagcgtaagt cataacaggt
     2881 catcagaccg accttcatcc cctccacctc gagtaacgga gcaatttcat taccagcatc
     2941 aacacggcgt gattcctgaa tggcaaatgc atcatagaga tgcaatttgg cataacgggc
     3001 gacgatgttt cctgcctgta gcgccaccag catattccat gcgcgccccg gcgttgaagg
     3061 aacatgaatc gtcagaattg tcgtcatcat gttacgttta ctttctcgcc gtaaacgtcc
     3121 gaggaattcg ccttccagca gctgtgctga tttaaccgat agatctgcat catgatcatc
     3181 gcgcgccagc aatgcttccg gcaggacaaa cagcgatacg tcgttttccg ccgcctgcgc
     3241 catcaacgag gcacaaatct cagcgttctt ttcccacaca gatgtaacag caaactgtcc
     3301 tgcagcaacc agcattgcca cctcctgtca gagtcgtaaa attcgccaac gacgattatt
     3361 cggcgttaca cttatcactc atacaaatca aatagcagga ttttgcagtg ttacaacttc
     3421 ttttagcagt ttttattggc ggtggtacgg gaagcgtggc gagatggctg ttaagtatgc
     3481 gatttaaccc gctgcatcag gcgattccgt tggggacgct ggcagcaaac ctgattgggg
     3541 cattcatcat aggaatggga ttcgcctggt tcagcaggat gacgaacatt gatccagtgt
     3601 ggaaagtatt aatcaccacc ggattttgtg gcggtctaac aaccttctca acattttcgg
     3661 cagaagtggt gtttttgtta caagagggcc gctttggctg ggcattactg aacgttttcg
     3721 tcaaccttct ggggtctttt gccatgaccg cactggcatt ctggctgttt tcggcctcaa
     3781 ccgcacacta aagaccaaaa aaaacccgct gattaagcgg gttttgaatt cttgctgacg
     3841 tatcttacag agcgattacg tttgcagcag aagggccttt ggcaccgtta gtgatttcga
     3901 actctacgcg ctgaccttca gcaagagttt taaaaccatt agtctggatt gcagagaagt
     3961 gtacgaacac gtctttgctg ccgtcttccg gagtaatgaa accgaatcct ttggactcat
     4021 taaaccactt aacgttacct ttaatcttag acatcaaaat tacctttaca tgaaaaatag
     4081 acacaaatgc tgtgtcggtt accagtacac caattgtggt acgctttgtc cagtgcaact
     4141 ttactaaaaa gtgataaaaa tcgcgttatt tttgatgtta cttattttag ttattgattt
     4201 taaagaagtt actaaaactt catttgtctc aaaactgaaa gcgcatccag gcaaagtaca
     4261 cattgccatt gttgtaggta cccggaatgt aggtcatctg aaaagtaact gggccataac
     4321 ccacggaggc caatggcagt agaaccggga gagggatgta attccagtta tcgcgtgccg
     4381 ttacgccagc ggtgaatccc agacctaaat gaaaattttc atccgccagc ggtcgccagg
     4441 tactttccca tccgtatccg gcaatcggtt cccatttgtt ccacgagtcc ttaaatgcca
     4501 tggcatacag gccatgccag tttccttttt catcccaacg cgacaggcca aaaccgccac
     4561 cccacggtcg ctcgttatag cgatcggttt tttctttgtc gtaagcgaaa cgtgcatgcc
     4621 aggtgatggc aggaatatat aaatcataat gttcaggctg ttgccaggtt tgtacaatat
     4681 tttctctaaa cgttgtcatc cactcatctg cgttagcaaa aactttacca acgctgatta
     4741 actgaataaa aacaaaggaa aagatagcga catatttact cacgttcatt tgtgaccata
     4801 aaacatttat caaaaatcta ctactagcat agcaaagcta caattaacat aaccttaaca
     4861 gccccaacat aaagaataat ctgaatagct gctctattta tttccgcttg ttcaagttaa
     4921 atatttccgc gcatcgattt aaaatgacac atcgttttct gcgatgggaa tagtcataaa
     4981 agaaaaagct agttgtttct gacaacaaaa tgcaaccaag aaaaaatgac ttatttttta
     5041 ataagatacc aaaaatcatc ttaacaggga atatattata tatcgtcaac ccacgcccat
     5101 tgcagattat tttttgagca cacaaccgca gctaacacca ttgcaattaa caaatttgca
     5161 tcaatcctcc atcaatttac acacattatt atgtgataat tgccaaccgc taaatatgcg
     5221 ttttgttatc tacgtataaa aacagcaact tcaatgtctt aatggcagtt tttcttgatt
     5281 ttaatcagca ttcatcgcca atttattggg catatttttt ccttaagctt taggaatttt
     5341 ttattattta ctttggggcc tggagacagg aaaaattatg ctgacattca ttgaactcct
     5401 tattggggtt gtggttattg tgggtgtagc tcgctacatc attaaagggt attccgccac
     5461 tggtgtgtta tttgtcggtg gcctgttatt gctgattatc agtgccatta tggggcacaa
     5521 agtgttaccg tccagccagg cttcaacagg ctacagcgcc acggatatcg ttgaatacgt
     5581 taaaatatta ctaatgagcc gcggcggcga cctcggcatg atgattatga tgctgtgtgg
     5641 atttgccgct tacatgaccc atatcggcgc gaatgatatg gtggtcaagc tggcgtcaaa
     5701 accattgcag tatattaact ccccttacct gctgatgatt gccgcctatt ttgtcgcctg
     5761 tctaatgtct ctggccgtct cttccgcaac cggtctgggt gttttgctga tggcaaccct
     5821 atttccggtg atggtaaacg ttggtatcag tcgtggcgca gctgctgcca tttgtgcctc
     5881 cccggcggcg attattctcg caccgacttc aggggatgtg gtgctggcgg cgcaagcttc
     5941 cgaaatgtcg ctgattgact tcgccttcaa aacgacgctg cctatctcaa ttgctgcaat
     6001 tatcggcatg gcgatcgccc acttcttctg gcaacgttat ctggataaaa aagagcacat
     6061 ctctcatgaa atgttagatg tcagtgaaat caccaccact gcccctgcgt tttatgccat
     6121 tttgccgttc acgccgatca tcggtgtact gatttttgac ggtaaatggg gtccgcaatt
     6181 acacatcatc actattctgg tgatttgtat gctgattgcc tccattctgg agttcctccg
     6241 cagctttaat acccagaaag ttttctctgg tctggaagtg gcttatcgcg ggatggcaga
     6301 tgcgtttgct aacgtggtga tgctgctggt tgccgctggg gtattcgctc aggggcttag
     6361 caccatcggc tttattcaaa gtctgatttc tatcgctacc tcgtttggtt cggcgagtat
     6421 catcctgatg ctggtattgg tgattctgac aatgctggcg gcagtcacga ccggttcagg
     6481 caatgcgccg ttttatgcgt ttgttgagat gatcccgaaa ctggcgcact cttccggcat
     6541 taacccggcg tatttgacta tcccgatgct gcaggcgtca aacctgggcc gtaccctttc
     6601 gcccgtttct ggcgtagtcg ttgcggttgc cgggatggcg aagatctcgc cgtttgaagt
     6661 cgtaaaacgc acctcggtac cggtgcttgt tggtttggtg attgttatcg ttgctacaga
     6721 gctgatggtg ccaggaacgg cagcagcggt cacaggcaag taaatagtaa tgccggaggc
     6781 aagtttcctc cggctttatc atgtcaccca ctgtggtata tgcgttgcgg tctgccaact
     6841 ttgccgtgaa caatttcagc aataatcaga tggcggctgg cgcaatattc aagataacgc
     6901 ctggcagtgg tgcgactgat ggtcagtgcc tgcgccaccg tttctgccgt atgttgcaca
     6961 ccaggctctt taaacagttt tcgcaccgcg tttagcgtca aggggtcaat gccggtcggc
     7021 agttcgtctt tgggttcacc gcgagcatag gcattaaaca tctcatcaat ttgcttctgg
     7081 ctggcgctat caatactttc cagcatatgt ttacgctggc ggaaacgggt tagcgtttgc
     7141 cccagccgtt cataggcaat gggcttaatg agataatcaa atacaccaca acgtacggct
     7201 tcagacaccg tttccatatc gctggctgca gtggtaaaca ccacgtcgcc gggataatgc
     7261 gcctgcacca gttcatgcag taaattaatc cctctaccgt caggaagata gttatcgagc
     7321 aagattagcc ccggcttaaa acgctcgatc atcattcggg cctgcgccag gtttcccgcc
     7381 agtaatatct gactgaatcc gggaatgtga cgaatatatt cagcatgcat ctctgccagc
     7441 ggcgtttcgt cctcaacgat caatagggtt aatggagctg tcatttggtt tcactttcgg
     7501 aatatagatt gaaaataagg taccgcaggg atcattatct tcgagagtga taacaccacc
     7561 gcagcgcgtt acgtagctgg caatcaagta caacccaatg ccatgttcac cgggctcgtc
     7621 agcacgcgta ctgaccccct gctcaaatat tttgtctcgt agagactctg gaacgccgca
     7681 gccctgatcg gcgacttcaa tcaccacatc atcgccttca tcgctgagga ataattcaac
     7741 gatcttgttt ccttcatcgc tacgcaggct ggcttcgaag gcgttatcaa gtaaattgcc
     7801 cacaatggct gcaaactcgg tgctatccag tcctggcggc agttgcgaaa gctggctacc
     7861 ggggacaatg atcattttta gccccagttc ccgggcgcgc tgcactttac caaaaagcag
     7921 ccccgccacc tggcgatcgg caaacgcctc gcgcaggctg tcaataagct gttgctgggc
     7981 ctgagactcc ccctgcacca tcgccagcac gcgatcatac tctttcatct gcaacagacc
     8041 attgagcgtc gacatccaat tgagatgctc gtgtcgcaat gtacgaaggc tctcaacgta
     8101 ttgttttatt tgcgtcagtt gcgcattgag ggtggaaatt tcgtctttac tgcgaaagct
     8161 gataatggcc cccagcaaat catcacctga acgaatagct tcccgattgg caataacgct
     8221 cagaccgtta aagttagcca ccacatcctg acgtttttca tcaatctgtt cggtaaagaa
     8281 atcggcgggc ctgaccactt cagcaatggg tttacccaac cattgccgtc cgggagagct
     8341 cagccccagc atctttcttg cgttacgatt gatggcggta atgtaaccat gcggatccac
     8401 cgcaatcagc ccttcataaa ccgaactaaa cagcgcctct tgctgacgga ccacgcgtgc
     8461 gatttgcttt ggctccatgc ccatcatttg ccgacggata tgcgctgcca ggaaccacga
     8521 cagcaacatc agaatcccta acagcacgac aaacacgcct gccatcggta ataaaaactc
     8581 agcccgccag ctatcgattt tactcaccag gtagccaatc gacaccacgc cgatgacttt
     8641 tccatcgtca tcaaagattg gcgttttggc gcgcatcgcc atccccattg accctttccc
     8701 ggtaatgaag tagctctccc ctttctccag cgcgccctgt ttggtgaact gcataggata
     8761 accaattttc tccggattag gatggtaaag gcggatcgag tgccggtccc caatcaccac
     8821 ataatcaaaa tcggtatctc tttgtaattt gttagcgatg gtcgccagcc gtttgtagtc
     8881 acgcgttttc acctcagaga tgacactgtc attggaggca ataattttcg cctgattcat
     8941 tgccatgtcg cgtacatgaa gcgttaaata gtcctcaaaa ctggccgtaa aatattgcgc
     9001 cagggctgca atgacaaata ttgagaacac cagaatcagc aaaaagatac gcagcggaaa
     9061 tgccagtctt tggaaaaatg caaactgttt attctcgtta agctgcaaca ttgttttcct
     9121 tgcgggttgt cccgagcctc tttgacggat gtaattaaaa tgt
//
LOCUS       DAZKIP010000154         9067 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZKIP010000154 DAZKIP010000000
VERSION     DAZKIP010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9067
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.155"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            464..697
                     /locus_tag="ACHOUK_004130"
     CDS             464..697
                     /locus_tag="ACHOUK_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899470.1"
                     /translation="MLKSTLIAKCLYQNRMVSSISIGESAVKSIFEEYFPGHDFNKWN
                     TKLPPAVSTRILKATERASTIRVNYFIKDLWDL"
     gene            complement(675..1082)
                     /locus_tag="ACHOUK_004131"
     CDS             complement(675..1082)
                     /locus_tag="ACHOUK_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899471.1"
                     /translation="MEFKDLPTPLQEMASNIVRSQLATLDLSTAEKETIDNMVRNVRN
                     AFSGLYGSDNQKQESDVNKRVISVCVNGHVLSSIKTETATVFDCLCIVQSLVDALFRS
                     VNLENDANLRGRIIAHPYAHTLGSVDIKDPTNL"
     gene            complement(1105..1323)
                     /gene="ydfC"
                     /locus_tag="ACHOUK_004132"
     CDS             complement(1105..1323)
                     /gene="ydfC"
                     /locus_tag="ACHOUK_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFP9899472.1"
                     /translation="MQKRDPVIIAPDYTDDELYEWMHQKIKAVQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            complement(1396..1695)
                     /locus_tag="ACHOUK_004133"
     CDS             complement(1396..1695)
                     /locus_tag="ACHOUK_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899473.1"
                     /translation="MALYIDISAIAGQVRVIRAVTKRYAPLLQKVSGECTEDIVNDFV
                     IELRGLIFSYKVTTIFADGSRETVRALRLKGCVKDLATTFWARKLDCIHNQFPLE"
     gene            complement(1959..2366)
                     /locus_tag="ACHOUK_004134"
     CDS             complement(1959..2366)
                     /locus_tag="ACHOUK_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9899474.1"
                     /translation="MKNETFGARLLYRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPVNLTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            2443..2670
                     /locus_tag="ACHOUK_004135"
     CDS             2443..2670
                     /locus_tag="ACHOUK_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFP9899475.1"
                     /translation="MLKIDAIAFFGSKTKLANVAGVRLASVAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRAGRLNNENHH"
     gene            2654..3205
                     /locus_tag="ACHOUK_004136"
     CDS             2654..3205
                     /locus_tag="ACHOUK_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFP9899476.1"
                     /translation="MKITTEQVCEALDTWVCRPGMTQEQATILITEAFWALKERPNID
                     VQRVTFNDGEVDQRALGVNRVKIFERWKAIDTRDKRDKFTALIPAIMEAIRISDFRLY
                     CEITDGKSITYMIAGLNKEYGDVVESGLLFADPVVVERETDELIEKAIAFKHAYRQQY
                     QYYFADKQMSARGAYEYRCTTMG"
     gene            3177..4217
                     /locus_tag="ACHOUK_004137"
     CDS             3177..4217
                     /locus_tag="ACHOUK_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFP9899477.1"
                     /translation="MSIDALRWAKKVKTGSSSSKSVLTWLADMCGADLCAYPSVSALA
                     EVTELNKKTVQDSLRHLMEIGLIVDTGERKGRTKQIVVYRLIGVEESVAEPEYTQKRE
                     SLKVGKIGAVNKNSTENGYVSAQNRPKNRTLSCMENNQRHPNFPSKTPKNGSRNPKEP
                     KDLNPTHNARESAPTSEQEVLSLQAAPPVFLYGLSEPIGKFPMSDSWYPSRDFRRRAA
                     LWGMALPETEFTPAELAAFRDYWAAEGKVFTQIQWEQKFARHVNHVRAQVKPVSKGVN
                     HAAAPGGTASRAVQEIRAAREQWERENGFISDGNGLEAVGTHGGGLFEPLDPEERGRT
                     FEALDCTDWRDD"
     gene            4210..4671
                     /locus_tag="ACHOUK_004138"
     CDS             4210..4671
                     /locus_tag="ACHOUK_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFP9899478.1"
                     /translation="MTEQQIRQVCRQCMDRCRAGETWPPDLAEFVALISKSGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWSQPVLYHICLEMRSKGIERQMTEGELKRLAE
                     RQLTKWAKHVSNGLSVPPVRRQLAAPKRPSGPTPIELLKQEYERRKAAGFV"
     gene            4759..5439
                     /locus_tag="ACHOUK_004139"
     CDS             4759..5439
                     /locus_tag="ACHOUK_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFP9899479.1"
                     /translation="MAARLDISREEVLNELWELKKAGFVDKSVYTWRVADNNVQQEQP
                     APEETTTATEAKISECDLTATIEQRGPQTADELATLFGTTSRKVASTLAMAISKGRLI
                     RVNQNGKIRYCIPGDNLPAEPKVESVAETDGKAFPQPAGVALPVQKDATQEDIKTETV
                     ADIVQSLPSFTATREDDLILPSLHMANRELRRAKNHVQKWERVCAALRELNKHRDMVA
                     GICRKSGQ"
     gene            5436..5600
                     /locus_tag="ACHOUK_004140"
     CDS             5436..5600
                     /locus_tag="ACHOUK_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899480.1"
                     /translation="MSGWCRPEIMILTMKVKCIGSLIGRSEAAVRMKAQVKGISLILR
                     GDFHQSTKYP"
     gene            5605..6138
                     /locus_tag="ACHOUK_004141"
                     /pseudo
     CDS             5605..6138
                     /locus_tag="ACHOUK_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498860.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            6299..7057
                     /gene="paa"
                     /locus_tag="ACHOUK_004142"
     CDS             6299..7057
                     /gene="paa"
                     /locus_tag="ACHOUK_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcfC family adhesin Paa"
                     /protein_id="HFP9899481.1"
                     /translation="MRNIMAGFLIFLSSAAYADINLYGPGGPHTALLDAAKLYAEKTG
                     IIVNVHYGPQNKWNEDAKKNADILFGASEQSALAIIRDHKDSFSEKDIQPLYLRKSIL
                     LVKKGNPKNIRSIDDLTRPGIGVIVNDGGGTSNTSGTGVWEDIAGRKGNIETVAAIRK
                     NIILYAPNSGTARKALENQPGADVWITWADWAASNPEIGDVVEIAPDYVIWRDMNITV
                     RQDANDETRRFAEWLQTDEAAPAFKKYGWTRKGT"
     gene            7393..7548
                     /gene="hokD"
                     /locus_tag="ACHOUK_004143"
     CDS             7393..7548
                     /gene="hokD"
                     /locus_tag="ACHOUK_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFP9899482.1"
                     /translation="MKQQKAMLIALIVICLIVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            7769..8026
                     /locus_tag="ACHOUK_004144"
     CDS             7769..8026
                     /locus_tag="ACHOUK_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899483.1"
                     /translation="MRIEELREIFSEDGLYTVRVEKGAIVSHCRIKCLQSQQRKSGAA
                     LIHFVDGLVTDGFILRANEFVTSLPSLKEAGIKAGFSAFED"
     gene            8096..8374
                     /locus_tag="ACHOUK_004145"
     CDS             8096..8374
                     /locus_tag="ACHOUK_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899484.1"
                     /translation="MAQNSRLHNSDNSAVFASRHGRRSHAFKSDWFRHAPCTEEQAEW
                     LIQNYRRRGYEFRKALSLDYRHWIIYVRLPYSERPPRPSRTFQQRIWR"
     gene            8376..>9067
                     /locus_tag="ACHOUK_004146"
     CDS             8376..>9067
                     /locus_tag="ACHOUK_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP9899485.1"
                     /translation="MRVLLRPVPVPELGVVVLKPGRESMQVFHNPRVLVEPEPKSMRG
                     LPSGIVPAVRQPLAEDKSLLPFFSNERVIRAVGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATIILKQR
                     TKNLAPLPHAHQ"
BASE COUNT         2439 a         2030 c         2354 g         2244 t
ORIGIN      
        1 cccataacac tgtttttagc tttaactgtt ccgcgtgcgc ttagccgcat tcactgcatc
       61 acaaaattca ctttaaaaag ggcggacatc agttcatggg caaacagatg ccgccaaacg
      121 tcaccagaaa attgataaca gagggcgttg cagcggggtt gtcacttaag cgtatggtca
      181 acctgacaac ccggtgtcct caacggggaa ggaataaccc cgccatactt accgctgcgc
      241 catttcgcgg agtgccacaa ccggaagcgc acggtcgacg aaaatttaac gacaggctat
      301 ctatgaacca gcaacctcgc cgtgcgcttt cgcgttatgc tctgactttt cagagaaata
      361 tcctttcagt aaactgtcag tgccggatgt tcacccgtgt ccggcgcacg cactccactt
      421 cacccgtgga gaactcctta attaccaacc ctcaggaggg tgaatgttaa aatcaactct
      481 tattgctaaa tgcctttatc aaaatcgcat ggtaagcagc atttcaatag gcgagtctgc
      541 agttaaaagt attttcgaag agtactttcc cgggcatgat tttaataaat ggaataccaa
      601 attaccgcca gcagtttcaa cgcgtattct gaaagcaacc gaaagagcaa gtacaattcg
      661 cgttaactat ttcattaaag atttgtggga tctttgatat ccacagagcc taaagtatgt
      721 gcatatggat gtgctattat gcgccctcgc agatttgcat cattttctaa attcactgaa
      781 cgaaacaggg catcaacaag gctctgtaca atgcaaaggc aatcgaagac tgtcgccgtt
      841 tctgttttga ttgatgaaag aacatggcca ttcacgcaaa cagaaattac ccgtttatta
      901 acatcgcttt cctgcttttg attatcagaa ccatatagcc cagaaaaagc attgcgcaca
      961 ttacgaacca tattatcgat ggtttctttt tctgcggtac taaggtcaag agtagccagt
     1021 tgtgaacgaa ctatattcga tgccatttcc tgtaatggcg ttggtaaatc tttaaattcc
     1081 attattagcc tcgttggtta gctattaacg tgggtatgta atcattctgg caatgcttaa
     1141 tgccgctgct ttttccagat tggtgatatc ctgctccaga gcggacagat tttcagcctg
     1201 cttagccctg gcttcattgg cccatttcag gtcctgcacc gccttaattt tctggtgcat
     1261 ccactcataa agttcatcat cggtatagtc tggcgcgatg atgacggggt ctcgtttctg
     1321 catgtcggct ccttgtggtt agcgttgcct gctttttaac cacgtcaggc gaggtggtat
     1381 cctctgaggg gtctgttact cgagaggaaa ttggttatga atacaatcaa gttttcttgc
     1441 ccagaatgtg gtggctaagt ctttgacaca tcctttaagc cgcagggctc tgacagtttc
     1501 gcgggagcca tctgcaaaaa ttgtggtcac cttgtaactg aagatgagtc ctcgcagttc
     1561 gatgacgaaa tcgttgacaa tatcttcggt gcactcacca gagactttct gaagtaaagg
     1621 cgcataccgc ttagttaccg ctctgataac tcttacctgt ccggcaatgg cgctgatatc
     1681 aatataaagc gccatcgctg tttctttgct gatccctgga cgccttccat tctgatgttt
     1741 gactcgccca ctgagaaatc ctctgcttcc ccttaacgcc gggtagcgga actgtttgct
     1801 gagaacaccg tgcggtgtct tgatgagtag aatttagaat agcctaagag ttatggtcaa
     1861 gctttttgtg tagaaaaacc taagcttctt gatgtaaaaa acacaagtat ttgaaagttt
     1921 gtgcttttta ttacagagag ttgcgaaaaa aaggggggtt atttatttgc gcttcttttg
     1981 cgagctttga gtagttcttc aaaaagtttg ttgaaattct caactcgagc acgcatctct
     2041 gacaacagag ccttttgctc tgactcaggc agtgcgtcga acagttgaag caactctttt
     2101 tgatcttctg tcagattgac tggctgatta tctgggatcg gttcgcctgg ttgcttatct
     2161 tcatctccaa aaagaagcca agtcggcgag cactgaagcg cctggctcag tgcgaataat
     2221 ctcttccccg ctggctgtgt ttcatctctt tcccattgag aaattgttac gtgagccact
     2281 ttgaccagct tacctaatgc ggcctgagac agttttaatt tttttcgcct gtataagagg
     2341 cgagcaccga aggtttcgtt tttcatatta ggtaattcta atttttcttg acttaggttt
     2401 ctctacgatc tagtttcctt aggaaaatct aagggtttcg atatgttgaa aattgatgct
     2461 atagcgtttt ttggcagcaa aacaaagctt gccaatgtcg caggagttag gctggcaagc
     2521 gttgctgcat ggggggaact ggttcctgaa ggtcgcgcga tgcgcctgca agaggcatcc
     2581 ggcggggaac ttcagtacga ccccaaagtt tatgacgaat atcgtaaggc aaagcgggcg
     2641 gggcggttga acaatgaaaa tcaccactga acaggtttgt gaggctctgg atacctgggt
     2701 atgccgacca ggaatgacac aggagcaggc gacgatatta atcacggaag cattctgggc
     2761 tctgaaagaa cgcccgaaca tcgatgttca acgcgtcacg tttaatgatg gcgaggttga
     2821 tcaacgggcg ctgggcgtta accgggtgaa gatattcgaa cgctggaaag ctatcgacac
     2881 cagagataag cgtgacaaat tcacggcgct gattccggca attatggagg ctatccggat
     2941 cagcgatttc agattgtatt gtgaaattac tgacggaaaa agcattacgt acatgatcgc
     3001 cgggttaaac aaagaatatg gcgatgtggt ggagtccggg ctgctttttg cggatccagt
     3061 tgttgtggaa cgtgagactg acgagcttat agaaaaagct attgctttca agcacgcgta
     3121 tcgtcagcaa taccaatatt actttgcaga taaacaaatg tctgccaggg gggcgtatga
     3181 gtatcgatgc actacgatgg gctaaaaagg tgaaaaccgg cagttcatcc agtaagtcag
     3241 tattgacctg gcttgctgat atgtgcggtg ccgatttgtg tgcatacccg tctgtatctg
     3301 cactggcaga agtaacggaa ctgaacaaaa agactgtgca ggacagctta cgacacctga
     3361 tggagattgg gttaattgtt gataccggtg agagaaaagg cagaacaaag caaattgtgg
     3421 tgtaccgact tatcggtgta gaagaaagtg ttgccgagcc tgaatacacc caaaaacggg
     3481 agtctttaaa ggtgggtaaa attggtgctg ttaataaaaa cagtaccgaa aatggttatg
     3541 tttcagcaca aaacagaccc aaaaacagaa ctcttagctg catggaaaat aaccaaagac
     3601 acccaaattt tccatcaaag acacccaaaa acggatcacg gaacccaaag gaacccaaag
     3661 atctaaaccc cacacataac gcacgcgaga gtgctccgac cagtgagcag gaagttttgt
     3721 cgttacaggc agccccccct gtattcctgt atggcctgag cgaacccatc ggaaaattcc
     3781 cgatgagcga tagctggtat ccgtcacggg attttcgacg acgggctgcg ttgtggggga
     3841 tggctttgcc ggagacagaa tttacacctg ctgaacttgc cgccttccgg gactactggg
     3901 cagcggaggg gaaagtgttt acgcagattc agtgggagca gaaattcgcc cgtcacgtaa
     3961 atcacgtcag ggcgcaggtt aaaccagtca gcaaaggggt aaaccatgca gcagcaccag
     4021 gtggcaccgc atcacgggca gttcaggaaa ttcgggcagc acgtgagcag tgggaacgtg
     4081 aaaacggatt tatcagcgac ggaaacggcc tggaagctgt gggaactcat gggggtggtt
     4141 tattcgaacc gctggatcca gaagaacggg gccgcacctt cgaagctctg gattgcacag
     4201 attggcgcga tgactgagca gcaaatccgg caggtctgcc gccagtgcat ggaccgctgc
     4261 cgggcgggtg aaacatggcc tccggacctg gctgagtttg tggcactgat ttcgaaaagc
     4321 ggagccaatc cattcggtct gacggtggat gctgtgatgg aggagtaccg ccgctggcgc
     4381 aatgagtcct ggcgatacga cggaagtgat aagtacccgt ggtctcagcc tgtgctgtat
     4441 cacatttgcc tcgagatgcg ttcaaagggg attgagcgcc agatgaccga aggggaatta
     4501 aaacggcttg cagaacggca actgacgaaa tgggcaaagc atgttagtaa cggcctgagc
     4561 gttccgccag tccggcgaca actggcggcc cccaaacgcc cgtcggggcc aacgccaatt
     4621 gagttgctga aacaggaata tgaacgccgg aaagcggctg gttttgtttg agttgagaag
     4681 tgatttttta ccgggaggaa atttatggag actgtttttg acgcactgaa agcgatggga
     4741 aaagccacgt cggtatagct ggctgcgcga cttgatatca gtcgtgaaga agtactgaac
     4801 gagctgtggg aactgaaaaa ggctggcttc gttgataaaa gcgtatacac ctggcgtgtg
     4861 gctgataaca acgttcagca ggaacagcca gcgccagaag aaaccaccac ggcaacagaa
     4921 gcgaaaatct cagagtgcga tttaaccgcg acgattgaac aacgcggacc acaaacggcg
     4981 gatgaactgg ctacgctgtt cggtacaaca tcccgcaaag ttgcttcaac gctggcaatg
     5041 gcaatcagca aaggtcgtct gattcgcgta aaccagaacg gtaaaattcg ttactgcata
     5101 ccaggtgata atttaccagc agagccgaaa gttgaatcgg tagcggaaac cgatggtaaa
     5161 gcctttcctc agccagccgg tgttgcgtta ccggtacaga aagatgcaac acaggaagat
     5221 attaaaacag aaactgtggc ggacattgtg cagtcgctgc catcgtttac tgcaacgcga
     5281 gaagatgatt tgattttgcc atcgctgcat atggcaaatc gcgaactgcg tagggcgaag
     5341 aatcatgtcc agaagtggga gcgagtctgc gccgcgctgc gggagctgaa caagcaccgg
     5401 gatatggttg ccgggatttg tcggaagtcc gggcaatgag cggatggtgc aggcctgaaa
     5461 tcatgatact aacaatgaag gtaaaatgca tcggcagtct gattggtcgt agtgaggcgg
     5521 cggtcaggat gaaagcccag gttaagggaa taagcctgat tctgcggggt gattttcacc
     5581 agtcaacaaa atatccgtag cgcgataacg gtcaaaaatt atggcgctga cacttttgtg
     5641 ccactggaga tgactgtacc taagttcagg ggagaagaac acgtccggtg ggatggtcgg
     5701 gccagattta aagggcaggt catggctcca gcctgtacgc tggcaatgga ggctgcctgg
     5761 cgggaaattg atatgggaac cacgccactc agggatttac tgccggtcca gagaataaat
     5821 tcctgttacg gttacaccac tgtgatcttg caagtgcagg aaagtaggtc tacacggtaa
     5881 cgcgagtgcg tgtaactttt gatgtcattc ccgtagaaac accggacaaa ttttcgctga
     5941 caggtcatgc agaaggtata aatctgcaga ttatggacaa ttacggatat ccggcaagag
     6001 ccggaaaaag catgccgcct ctaattctca gtggaagatg gacttgatta tactcattgc
     6061 attgtcagaa atagttatcc attaaaggct ggctattcca aacaggatgt tgattacaaa
     6121 aatgtaatca acatgtaagg tttatactct tcaatatgcg tataattttc cttattttgt
     6181 tgactttaaa taacaagcta tgcacgaggt aaagtcggat aagtttatct ggatgtaata
     6241 tatattattt gtagtgttta taactttatt tcatgataac caataaaagg agttttttat
     6301 gaggaacata atggcaggtt ttttaatatt cctgtcttct gctgcttatg ctgatatcaa
     6361 tctgtatggt cctggtggcc cgcatacagc cttgcttgat gcagccaaac tttatgccga
     6421 aaaaacaggt attatagtga acgttcatta cggcccacag aacaaatgga atgaagatgc
     6481 caaaaaaaat gcagatatct tgtttggcgc atcagaacaa tctgctctgg ctatcattcg
     6541 ggaccataaa gacagcttca gtgaaaaaga tattcagcct ctttatctgc gaaaaagtat
     6601 tttactggta aagaaaggta atcctaaaaa tatccggagt attgacgacc tgaccagacc
     6661 tgggattggc gtaattgtta atgatggtgg tggtaccagt aatacatcag gcactggcgt
     6721 ctgggaagat attgccggac gtaaagggaa tatagaaact gtcgccgcaa tccgaaaaaa
     6781 tattatttta tatgcgccca atagcggaac tgcacgtaag gctcttgaga atcagcctgg
     6841 agcagatgtc tggataacct gggctgactg ggcagccagt aatccagaaa ttggtgatgt
     6901 cgtggaaata gcgccagact acgtgatatg gcgtgatatg aacattacag tacgtcagga
     6961 tgcaaatgat gaaacccgtc gatttgcaga atggctacaa accgatgaag cggcgcctgc
     7021 attcaaaaaa tatggctgga ccaggaaagg cacttgacat cctcgtcctt caggacgtgg
     7081 attctttttc cggatgccgc gccagcggca tgtaggggca gctcacaaaa cggaaaaaat
     7141 tgtacgctaa gcctcgccag gtgaactgaa ttcattccga tatgggaatt cccatatcgg
     7201 gcgaaaacgg tttgctgtaa cggcagagtt aagtaggatt gctgcgggtg cttgaggcta
     7261 tctgcctcgg gcatgaacac caacggcaga tagataaaag ccccacccga ctataaatcg
     7321 aagtgaggcc cctatatgct cgtcacatat agattgcctc ttacggaccg aaaggtcaag
     7381 gagaagcagg ctatgaagca gcaaaaggcg atgttaatcg ccctgatcgt catctgttta
     7441 atcgtcatag tgacggcact ggtaacgagg aaagacctct gcgaggtacg aatccgaacc
     7501 ggccagacgg aggtcgctgt cttcacagct tacgaacctg aggagtaaga gaccaggcgg
     7561 gggagaaatc cctcgccacc tctgatgtgt caggcatcct caacgcaccc gcacttaacc
     7621 cgcttcggcg ggtttttgtt tttattttca acgcgtttga agttccggac ggcgccggaa
     7681 tagaatcaaa aatacttaag tagcgcgcag ggagaagagg gatggacccc gaacagggga
     7741 gtgctattta tctggaagga ttctgttgat gagaatcgaa gaattacgtg aaatttttag
     7801 tgaagatggc ctctatactg tgcgcgttga gaagggcgct attgtcagcc actgccgtat
     7861 taaatgttta cagtctcaac aaaggaagag tggagctgcg ttaattcatt ttgtggatgg
     7921 gcttgtgacg gatggtttta ttttgcgtgc aaatgaattt gtcacatcgt tgccgtctct
     7981 gaaagaagct gggattaagg ctggtttttc tgcttttgaa gattagtgaa ttcatctaca
     8041 attcagcgca gggctgaacc cctgttgagt aacactgtgc caccggagaa agccgatggc
     8101 gcaaaattcc agactacaca attctgataa ttcagccgtc tttgccagca ggcacgggcg
     8161 gcgttctcat gcattcaaat ctgactggtt ccggcacgcc ccatgcactg aagaacaggc
     8221 cgaatggctg attcagaact accgcagacg tgggtatgag tttaggaaag ccctcagcct
     8281 cgattatcgt cactggataa tctacgtcag gctgccgtac tccgagcgcc caccgcgtcc
     8341 gtcccgcaca ttccagcaac gcatctggag gtaacgtgcg ggtattactt cgacctgttc
     8401 cggtaccgga acttggggtg gtggtcctta aaccaggccg tgaatccatg caggtattcc
     8461 ataaccctcg agtgcttgtg gagccggagc cgaaaagcat gcgcggtctg ccgtccggca
     8521 tcgtccctgc cgttcgccag ccgctggcgg aggataaatc attactgccg tttttcagca
     8581 atgagcgtgt gattcgtgct gttggtggcg ctggtgcact gtctgactgg ctgttgcgtc
     8641 atgtcaaatc ctgccagtgg cctcatggtg actatcatca cagtgaaacc gttattcacc
     8701 gttatggtac cggcgcaatg gtgttgtgct ggcactgcga caaccagctg cgtgaccaga
     8761 catccgaatc actcgagcaa cttgctcatc aaaacctgtc agcatggatg attgacgtca
     8821 tcggtcacgc aataagcggt acgcaggagc gtgaattatc tctggctgaa ttatcctggt
     8881 gggcggtccg caatcaggtg gcggacgcgc taccggaagc ggtattacgt cgttcgctgg
     8941 ggttgcgtgc ggaaaaaatc cgctcaatgt accgtgaaag cgacatcgta ccgggagagc
     9001 agaccgccac catcatactg aagcagcgca caaaaaatct tgcgccgctg cctcacgccc
     9061 accagca
//
LOCUS       DAZKIP010000155         9063 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZKIP010000155 DAZKIP010000000
VERSION     DAZKIP010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9063
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.156"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            37..399
                     /locus_tag="ACHOUK_004147"
     CDS             37..399
                     /locus_tag="ACHOUK_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899486.1"
                     /translation="MNEFEKIFNEMNLDRALLPILFRSNRSTVWKYLSGDSTAPASAM
                     SLIMLLQLIQKRNPDLLAEWLTLSDFTIPPEVYLDQPDYWKGWVYTQHKVNKNVLEYL
                     KKHYPDEDQKSMSKGREE"
     gene            complement(578..1036)
                     /gene="ybbJ"
                     /locus_tag="ACHOUK_004148"
     CDS             complement(578..1036)
                     /gene="ybbJ"
                     /locus_tag="ACHOUK_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFP9899487.1"
                     /translation="MMELIVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAVLTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLIIRAVIA"
     gene            complement(1033..1950)
                     /gene="qmcA"
                     /locus_tag="ACHOUK_004149"
     CDS             complement(1033..1950)
                     /gene="qmcA"
                     /locus_tag="ACHOUK_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFP9899488.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            2096..2773
                     /gene="fetA"
                     /locus_tag="ACHOUK_004150"
     CDS             2096..2773
                     /gene="fetA"
                     /locus_tag="ACHOUK_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFP9899489.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            2760..3539
                     /gene="fetB"
                     /locus_tag="ACHOUK_004151"
     CDS             2760..3539
                     /gene="fetB"
                     /locus_tag="ACHOUK_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFP9899490.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(3602..4456)
                     /gene="cnoX"
                     /locus_tag="ACHOUK_004152"
     CDS             complement(3602..4456)
                     /gene="cnoX"
                     /locus_tag="ACHOUK_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFP9899491.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(4517..5326)
                     /gene="ybbO"
                     /locus_tag="ACHOUK_004153"
     CDS             complement(4517..5326)
                     /gene="ybbO"
                     /locus_tag="ACHOUK_004153"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFP9899492.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRRSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(5316..5939)
                     /gene="tesA"
                     /locus_tag="ACHOUK_004154"
     CDS             complement(5316..5939)
                     /gene="tesA"
                     /locus_tag="ACHOUK_004154"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFP9899493.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFLMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            5910..6596
                     /gene="ybbA"
                     /locus_tag="ACHOUK_004155"
     CDS             5910..6596
                     /gene="ybbA"
                     /locus_tag="ACHOUK_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFP9899494.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            6593..9007
                     /gene="ybbP"
                     /locus_tag="ACHOUK_004156"
     CDS             6593..9007
                     /gene="ybbP"
                     /locus_tag="ACHOUK_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFP9899495.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGSENQLDSYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVTPLKAF
                     LAEHQIVPESFYPVVRARLTAINGKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVTLGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         2053 a         2350 c         2509 g         2151 t
ORIGIN      
        1 atttttaaga ggtttacgcc aggcaggaaa atttaaatga atgaatttga gaagattttt
       61 aatgaaatga atctggaccg ggcattgcta ccgatccttt ttcgttcgaa ccggtcgaca
      121 gtgtggaagt atttaagcgg cgatagtacg gctcctgcct ctgccatgtc gctgattatg
      181 ctgttgcagt tgatacagaa acgaaacccc gatctgttag ccgaatggtt aacgctttct
      241 gatttcacca ttccgccaga agtttacctg gaccagcctg attactggaa agggtgggtg
      301 tatacgcaac acaaggtcaa taaaaacgtc ctggaatatc taaaaaaaca ctatccggat
      361 gaagaccaaa aaagtatgag taaaggccgg gaagagtaaa ttgccaatgc cagttaacat
      421 ttaacatggc ggaggcggcc tacggttttg gcgtgatatg acgcgcccgc gtcgcatcag
      481 gcgtcggtgc acgactgccg gatgcggcgt aaacgcctta tccggcctac gattcgggcg
      541 cagacttccg ggcatgataa gacgcgtcag cgtcgcatca ggcgatgacc gcacggatga
      601 tcagcgttat cccttctatc gcaatgactt caacatgcgt acctgcgccg agatcctcgc
      661 tggcgctgac aggccatgaa ctgtcaccga cgcgcatatg accgcgcccg ttgaccagcg
      721 gagattccag cacaaaacgt cggccaatca gctgctgccc gcgctggttt aaatgactgt
      781 cgctgtgctt ttgttcgcgc acccgccgcg acaaccattt ccaccacagc caggcggcga
      841 gcagcgtcag gacggcaaac atcacccctt gccactccca acccagcggc accagccaga
      901 ccaccaggcc agtaatcact gctgccacgc cactccacaa caaataacca tttccgccca
      961 gcatctcggc tgccagcagc aaaccgccga gactgagcca gaaaatatgt ggatgaacga
     1021 ctattaactc catcatggct gagtccgctt gttggcgctg tctttcacca gctcggcaat
     1081 cccggcaatc gaccccatca ggctgctggc ctctaatggc atcatcacta ctttgctgtt
     1141 actggaggaa ccgatctgct gtaacgcttc ggtgtatttc tgcgctacga agtagttcac
     1201 cgcctgaata tcaccggagg cgatggcttc agacaccatt ttggtggcgc gggcttctgc
     1261 ttcagcggaa cgttcacgcg cttcagcctg taaaaacgcc gactgacgtt cgccttccgc
     1321 tttcaggatt tgcgactgtt tttcaccttc ggctttgagg atttccgcct gacggatccc
     1381 ttccgcttca agaatgtaag cgcgtttggt acgttccgct ttcatctgcg cgttcattga
     1441 agagataagc tctgccggtg ggcgcacgtc gcgaatttca atacgggtga ctttaatccc
     1501 ccacgggttg gtggcctcat cgacaatacg cagcaggcgt gagttgatgc tgtcgcgctg
     1561 agagagcatt tcgtcaagtt ccattgaacc caacacggta cggatgttag tcatggtcag
     1621 gttgatgatc gccagctcca gattgctgac ttcataagcc gcgcgtggcg cgtcaatcac
     1681 ctgaataaaa cagactgcgt cgatggtaac gttggcgtta tctttcgaga taacttcctg
     1741 ggaagggata tcgagcactt gctccatcat attgatcttg cgaccaatgc gatccataaa
     1801 cggcaccacc agactgagcc ccggctgtaa cgttttggta tagcgaccaa aacgttctac
     1861 cgtccattga tagccctgcg gcacgatttt gacgcccgcg ccgacaatga ccagcgcgac
     1921 aaaaatgaga atcgggataa agataagcat cggaaaaacc tcctgttgta ccgtccataa
     1981 tcagcaaaat tgctgcttga ttaaacaaat tatacctgat tactgaaaga gagttccccc
     2041 ttattcctgc gaaggataaa ctgtttttag taaaaatcag aaaaagggaa cagcgatgca
     2101 ggaaaatagt cctttgcttc agctacaaaa cgtaggatat ctggcgggtg atgcgaagat
     2161 tcttaataac atcaattttt cgctgcgtgc tggcgaattt aagttaatta ccggtccttc
     2221 tggttgtggc aaaagtacgc tgctaaaaat agttgcttca ttgatcagcc caaccagcgg
     2281 aacgttactg tttgaaggtg aggatgtcag cacactaaag ccagaaatct accgtcagca
     2341 agtctcttac tgcgcccaga cgccgacgct gtttggcgat acggtatacg ataatctgat
     2401 ctttccctgg cagatccgta accagcagcc tgacccggcc atttttctcg attttcttga
     2461 acgcttcgcc ttgccggaca gcattttgac gaagaatatc gccgagctat ctggtggtga
     2521 aaaacaacgc atctcattga ttcgtaacct gcaatttatg ccgaaggttt tattgctgga
     2581 tgaaatcacc agtgcgctgg atgaaagtaa taaacataac gttaatgaga tgatccatcg
     2641 ttatgtgcgc gagcaaaata ttgccgtgct gtgggtgaca cacgataaag acgaaattaa
     2701 tcatgcggat aaagtgatta cactgcaacc gcatgccgga gaaatgcagg aagcacgcta
     2761 tgaactcgca taatattact aacgaatcat tagcactggc attaatgctg gtagtggtgg
     2821 caatcttaat tagccataaa gaaaaactgg cgctggagaa agatattctc tggagcgtcg
     2881 ggcgcgcgat aattcagctg attattgttg gctatgtgct gaagtatatt ttcagcgtgg
     2941 atgatgccag cctgacatta ttgatggtgt tatttatctg ctttaatgcg gcgtggaacg
     3001 cgcaaaaacg cagtaaatat attgctaaag cttttatctc atcgtttatt gccattacgg
     3061 tcggggcggg aattaccctg gcggtgctga ttctctcagg gtcgattgaa tttatcccga
     3121 tgcaggtgat ccctatcgcc gggatgattg ccggtaacgc catggtagcg gtggggttgt
     3181 gttacaacaa tttagggcaa cgggtcatta gcgaacagca acagattcag gagaaactga
     3241 gtcttggcgc gacgccgaag caggcttcag cgatattgat tcgcgacagt attcgcgcgg
     3301 ctttaattcc gacggtcgat tcagcaaaaa cggttggctt agtgagttta ccaggaatga
     3361 tgtccgggct gatatttgcc gggattgatc cggtgaaggc gattaaatat cagattatgg
     3421 tgacctttat gctgctctca accgccagct tgtcgaccat aattgcctgc tatttaacct
     3481 atcgtaagtt ttataattcg cgccaccagt tggtggtgac gcaattgaag aagaaatgat
     3541 gcgatgccgg atgcgcagca tcgcatccgg cgttgtggtt catgtgccga tcaacggaat
     3601 atcaatacaa caatgcatac agctggcggc gatacttcga cgccagtgca tcacccgtac
     3661 ccagcgcagc gaggatctcc tggaacgttt tacgcgtctg accgtctgcg gcggtgagat
     3721 ctttacgcag atgcccgaac agcaactcca gcgcctcttc attgcgccca acctgatgca
     3781 gttgcagcgc cagttgcgtc gccagtgcgg catcttctgg attctccgcc acctgctgtt
     3841 gcaactgttg aatttccggc gtatcagccg cctgcttcag cagttcgatt tgcgccacca
     3901 gcccctggta gcgggtgtcc tgatcctgca acggaatggt tttcagcacc gcttccgcat
     3961 cttcagaacg gttcagcgca atcagcgttt ctgccagcag caggccgatc tccccgttct
     4021 gattcgacaa ctgccaggcg tctttcagca atggcagggc atcggtgtaa ttgccttcct
     4081 gcatcagttg catcgcctgc tgcgctttca gctcttcttc gcgcggcagc actttatcca
     4141 gcagggcgcg gatcgcctct tccggttgcg gcccctggaa gccatctacc ggttgcccgt
     4201 tctggaacag atacacggtc ggaatcgcgc gcagaccaaa ctgggcggca atcatctgct
     4261 ccgcgtcgca gtccagcttc gccagaataa attgcccgtt gtactgcgcc gcaaggcttt
     4321 ccagaattgg ggttaactgc aaacagtgct ggctacgttc agaccaaaaa tagaacagca
     4381 ccggagtggt catcgactgt tcaagaacct gttgcaggtt agattcgtta atgttgacaa
     4441 tattttctac ggacatggag tcgctctctg ttgtcgatat tttctttgac atgggggctt
     4501 aagcgcgcgc ttcaactcac ccctgcaata ttttgtccat cacgcgcccc ggcagcaggc
     4561 gcttaagcac cattaccgcc caggtcacca gcgttaccgg atagcgcatc ttcggcttct
     4621 cgctaataaa agcatggcgt actttgtcca ccaccgcttc cggtcccaac gtaaagcggg
     4681 cggcgatgcc gggattttcg actggtttat cactttgcgt ctggttgacg ttgtcggtga
     4741 agcgagtacg aatgggaccg ggttcgatca ggctgacttt aattccgctg cggcgcagct
     4801 ccatgcgcag cgcgtctgac cacgcctcca gcgcatattt actggccgcg taagcgccac
     4861 gacccggtgt ggagattaat cccatcaccg atgaggtcat cacaatacgc ccttcaccgt
     4921 gcggcaacat cgcgggtaac aggcgcatgg tgagctggtg tgcgccgaaa aagttggcgg
     4981 aaaactgctg ttccatctgc gcacggctga tggtggaaag ggggccatac atgccgaatc
     5041 cggcattgtt aaagatccca tacagacaat tatcggtcag ggcgatcacc tcgtcggctg
     5101 cgcgatcaac actttctggt gaatccagat cgatcaacac gccggtaaat cccatgctgt
     5161 tcatgcgctc aacatcatcc ggtttccggc aacctgccag cacatgaaaa ccctggcgtt
     5221 ttaattcgag cgcgctttcc aggccaattc cactggaaca tccggtaatt aagaccgatt
     5281 tttgcataac tttacctgtc aggatctccg ttgctttatg agtcatgatt tactaaaggc
     5341 tgcaactgct tcgccatcca gtcggcaata aacggctggg cgtcgcggtt gggatgaata
     5401 ccgtcatcct gcatccattg tggcttgagg tagacctctt ccattaaaaa gggcagcagc
     5461 ggaacatcaa actctttggc gagtttgggg taaatggcgc taaaggcttc attataacgg
     5521 cgaccatagt ttgcaggcag acgtatttgc attaacaatg gttcagcgtt ggcggctttg
     5581 acatcctgca aaatctggcg cagcgtttgc tcggtttgct gtggctgaaa accacgcaaa
     5641 ccgtcattgc cgcccagttc aaccagcacc caacgcggct gatgctgttt cagcagagcc
     5701 ggaaggcgcg ccagcccttg ttgcgaggtg tcgccgctga tgctggcatt gactaccgac
     5761 gttttactct gccacttatc attcaacaag gcaggccagg ccgcgctggc agacattcga
     5821 tacccggcgc tcaggctatc acccagaatc aataacgtgt ccgctgcggc agcacggaag
     5881 gttaacagga ccagaaacag gaagggcaaa tgccagcgga aaacattgtt gaagttcatc
     5941 atcttaagaa gtccgtcggt cagggggagc atgaactctc catcctcacc ggagttgagc
     6001 tggttgtcaa acgtggcgag accatcgcac tggtgggcga gtcgggatcg ggtaagtcaa
     6061 ccttgctggc gatcctcgcc gggctggatg acggcagcag tggcgaagtg agtctggtgg
     6121 gacaaccgct acataatatg gacgaagaag cgcgggcaaa gttgcgcgcg aagcacgtcg
     6181 gctttgtttt tcagtcattt atgttaattc ctacccttaa cgcgctggaa aacgtcgagc
     6241 ttccggcgct gctgcgcggt gagagtagcg cggaaagtcg taacggagcg aaagcgttgc
     6301 tcgaacagtt agggctgggt aaacgtctgg atcatcttcc ggcacagctt tccggcggtg
     6361 aacagcaacg agtggcgctg gcacgagcct ttaacggtcg acctgatgtg ctgtttgccg
     6421 acgaacccac cggtaacctt gaccgccaga cgggcgataa aattgccgac ctgctgtttt
     6481 ccctcaaccg tgagcatggc accacgttga ttatggtgac ccacgacctg caactggcgg
     6541 cacgctgcga ccgctgctta cggctggtga acgggcagtt gcaggaggaa gcatgattgc
     6601 acgctggttc tggcgcgaat ggcgctcgcc gtcgctatta attgtctggc tggcgctaag
     6661 cctggcggtg gcctgcgtgc tggcgctggg caatatcagc gatcgcatgg agaagggctt
     6721 aagccaacaa agccgggagt ttatggcggg cgatcgggcg ttgcgcagtt cgcgcgaagt
     6781 gccgcaagcg tggctggagg aagcgcaaaa gcgcggcctg aaagtcggca agcagctgac
     6841 tttcgccacc atgacatttg caggcgacac accgcagctg gcgaacgtta aagcggtgga
     6901 tgatatctac ccgatgtatg gcgatctgca aaccaatccc cctggcctga aaccgcaggc
     6961 gggcagcgta ttgctggccc cacgcctgat ggcactgctt aacctgaaaa cgggcgacac
     7021 cattgacgtg ggcgatgcca ccttgcggat tgccggagaa gtgattcagg aaccggattc
     7081 cggttttaac cccttccaga tggctccgcg tctgatgatg aatctggcgg atgtcgataa
     7141 aaccggagcc gtgcagccgg ggagtcgggt cacctggcgc tataaattcg gcggcagcga
     7201 gaaccagctc gacagctatg agaaatggtt gttacctcag cttaaacccg aacaacgctg
     7261 gtacggtctg gaacaggacg aaggcgcgct ggggcgatcg atggaacgct cgcaacagtt
     7321 cctgctgctt tcggcacttc tgaccttgct gctggcagtg gcagcagtgg cggtagcgat
     7381 gaatcattac tgtcgcagtc gctacgatct ggtggcgatc ctcaaaacgc tgggggcagg
     7441 gcgagcgcaa ctgcgtaagc taatcgtcgg tcagtggttg atggtgctga cgctttcagc
     7501 cgttaccggt ggggcgatag gcctgttgtt cgaaaacgtg ttgatggtgc tgctcaagcc
     7561 cgttctgcct gctgcactac cgccagccag cctctggcca tggctatggg cgctgggcac
     7621 catgacggtc atctcgctgc tggtggggct acgaccatac cgcttgttgc tggcaacgca
     7681 gcctttacgc gtattacgta atgatgtggt tgcgaacgtc tggccgctga agttttatct
     7741 gccgattgtc agtgtggtgg ttgtgctgct gctcgccgga ttaatgggtg gcagcatgct
     7801 gctttgggcg gtgctggcgg gcgcggtagt actggctttg ctgtgcggtg tgctgggctg
     7861 gatgctgctg aatgtacttc gccgcatgac gctgaaatcg ctgcctctgc gcctggcggt
     7921 tagccgcctg ttacgtcagc cgtggtcaac gttaagccag ctttcggcat tttcgctctc
     7981 ctttatgctg ctggcactgc tactggtgct gcgtggcgat ctgctcgacc gctggcagca
     8041 gcagttaccg ccagaaagcc cgaactactt tttaatcaac atcgccacag aacaggttac
     8101 gccgctaaaa gcgttcctcg cggaacatca gatagtcccg gaatcgtttt atccggtggt
     8161 gcgagcgcgg ctgacggcga ttaacggcaa gccgacagaa ggcaatgaag atgaggcgct
     8221 caaccgcgaa ctcaatctta cctggcaaaa tacgcggccc gatcataatc cgattgtcgc
     8281 cggtaactgg ccgccaaaag ctgatgaagt ttcgatggaa gaggggctgg cgaaacgctt
     8341 aaacgttacc ctcggcgata ccgtgacttt tatgggcgat acccaggagt tccgcgctaa
     8401 agtgaccagc ctgcgcaaag tggactggga aagtctgcgg cctaatttct attttatttt
     8461 ccctgaaggg gcattagacg ggcaaccgca gagctggctt accagtttcc gctgggagaa
     8521 tggcaacggc atgttgacgc aactcaaccg ccagttccct accattagcc tgttagacat
     8581 tggcgcgatt ttaaaacagg tcggtcaggt gctggagcag gtaagtcggg cgctggaggt
     8641 gatggtggtg ctggtcaccg cctgcggtat gttgctgttg ctggcacagg tgcaggtggg
     8701 aatgcgtcag cgtcatcagg agctggtggt gtggcgcaca ctcggtgcgg ggaaaaaact
     8761 gctgcgtacc acgttgtggt gtgagttcgc catgcttggg tttgtttccg gcctggtggc
     8821 cgcaattggt gcggaaacgg cactggcagt gttgcaggcg aaagtgtttg attttccgtg
     8881 ggagccagac tggcgattgt ggattgttct gccgtgcagc ggtgcgctgc tgctgtcgct
     8941 tttcggcggc tggctgggcg cgagactggt taagggtaag gcgctgttca ggcagtttgc
     9001 ggggtgatga gagtaaataa ggggcgttct ggttgaatgg aacgccttgt ttaaattaat
     9061 tct
//
LOCUS       DAZKIP010000156         8987 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZKIP010000156 DAZKIP010000000
VERSION     DAZKIP010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8987
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.157"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(192..1385)
                     /gene="tyrB"
                     /locus_tag="ACHOUK_004157"
     CDS             complement(192..1385)
                     /gene="tyrB"
                     /locus_tag="ACHOUK_004157"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFP9899496.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAVPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLAMLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(1638..2717)
                     /gene="alr"
                     /locus_tag="ACHOUK_004158"
     CDS             complement(1638..2717)
                     /gene="alr"
                     /locus_tag="ACHOUK_004158"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFP9899497.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKVGEPVGYGGTWISERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(2770..4185)
                     /gene="dnaB"
                     /locus_tag="ACHOUK_004159"
     CDS             complement(2770..4185)
                     /gene="dnaB"
                     /locus_tag="ACHOUK_004159"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFP9899498.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISSYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4268..5251
                     /gene="qorA"
                     /locus_tag="ACHOUK_004160"
     CDS             4268..5251
                     /gene="qorA"
                     /locus_tag="ACHOUK_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFP9899499.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(5417..5659)
                     /gene="pspG"
                     /locus_tag="ACHOUK_004161"
     CDS             complement(5417..5659)
                     /gene="pspG"
                     /locus_tag="ACHOUK_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFP9899500.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(5793..6830)
                     /gene="dusA"
                     /locus_tag="ACHOUK_004162"
     CDS             complement(5793..6830)
                     /gene="dusA"
                     /locus_tag="ACHOUK_004162"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFP9899501.1"
                     /translation="MHDNHEAQKINQTSVMPEKTGVYWNSRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            6919..8016
                     /locus_tag="ACHOUK_004163"
     CDS             6919..8016
                     /locus_tag="ACHOUK_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9899502.1"
                     /translation="MAYYNIEKRLKSDGTPRYRCNVIIKEKGVITYRESKTFPKHAHA
                     KTWGTQKVMELDLYGIPSSNAVDGLTVRDLLHKYLNDPNAGGKAGRTKRYVLELLMDS
                     DISAIKLSELTENDVIEHCRLRNNAGAGPATVSHDVSYLGSVLDAAKPVYGINYTSNP
                     AKSARPYLLKLGLIGKSNRRNRRPASDELDMLIEGLQQRSTHKCSKIPFVDILKFSVW
                     SCMRIGEVCRLRWEDLDQEQKSILVRDRKDPRKKEGNHMKVALLGEAWDIVQRQPKKS
                     EFIFPYNSTSVTAGFQRVRSKLGIKDLRYHDLRREGASRLFEAGFSIEEVAQVTGHRS
                     LNVLWQVYTELYPKSLHNRFEELQKSRNKTS"
     gene            8078..8326
                     /locus_tag="ACHOUK_004164"
     CDS             8078..8326
                     /locus_tag="ACHOUK_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFP9899503.1"
                     /translation="MRIEICIAKEKMTKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWKSADEWFVH"
     gene            complement(8337..8456)
                     /locus_tag="ACHOUK_004165"
                     /pseudo
     CDS             complement(8337..8456)
                     /locus_tag="ACHOUK_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
     gene            complement(8487..>8987)
                     /locus_tag="ACHOUK_004166"
     CDS             complement(8487..>8987)
                     /locus_tag="ACHOUK_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFP9899504.1"
                     /translation="RPMQVNGYFMGSLNQDGLSNDNIQIGLQYIEHIERTLNHGSLTS
                     REVTVLREIEMLENMELLSNYQLEELLDKIEVCAFNVEHAQLQVPESLRTCPVTLCEP
                     EDGVFMRNSMNSNVCMLYDKMALIHLVKTRAAHPLSRESIAVSMIVGRDNCAFDPDRG
                     NFVLKN"
BASE COUNT         2410 a         2285 c         2121 g         2171 t
ORIGIN      
        1 tactccacac gaaaaaaaca gggaaaggaa ttccagataa ttgggataaa cttaacaata
       61 aaagaactaa caacgtttat tatctctcac gtagaacgat ggtatcaaaa aaatcatcta
      121 taaagaaaga gtgaaagcga cgacagttta atttcactgc aggctgggta attccagcct
      181 gctttcctgc attacatcac cgcagcaaac gcctttgcca cacgttgcac atttgccgta
      241 tttaacccgg cgacacacat gcgaccgctg gcgatgagat agacaccaaa ttcttcacgt
      301 agtcggtcaa cctgagcggc acttaaaccg gtataactga acatgccgcg ctgattaagc
      361 agataatcga aattgcgttc tggcatctct gtgcttaata ccttcaccag ttcctgacgc
      421 attgccagaa tgcgagtacg catctcttct acttccgcca gccagctggc tttcaatgcc
      481 tcgtcattca gcactgcagc caccacctgc gcaccaaaat tcggcgggct ggagtagttg
      541 cggcgaactg ttgctttcaa ttgccccagt acgcggcctg cagcttcggc atcttcacac
      601 agaacagaaa gtccgccgac gcgctcgccg taaagggaga aaattttcga gaacgaattg
      661 ctcaccagag cgggtaatcc agcgctggca atggcgcgaa tggcgtaggc atcctcttcc
      721 ataccggcac caaatccttg ataggcaata tcaaggaatg ggataagctc gcgggctttg
      781 agaatttcaa tcaccgcatc ccactggtca ttagtgagat cggcacccgt tgggttgtgg
      841 caacatggat gcagcaacac aatactgcgg gcaggtaatg ttttcagcat cgccaacagg
      901 tcattaaagc gcacgccgtt agtcgcttcg tcataccagg ggtaagtgct tacttcgaat
      961 ccagccccgg cgaatattgc tacgtggttt tcccaggtag gatcgctgac ccagacgcct
     1021 gattccggga agtagcgttt caggaaatcc gcgcccactt tcaatgcccc tgagccgcca
     1081 agggtttgaa tggttgctac gcgctgttgt tgcagtaccg gatggtcggc accaaacagc
     1141 agcggcgcaa tggcatggcg atagctgtta agcccttcca tcggtaaata aagcgaagcg
     1201 ccatgaggca ccgcattcag gcgcgcttcc gcctccgcca cggctttcaa ttgtggaata
     1261 attccgtctt cgttgtagta cagaccgata cttaaattca ctttgtcgct gcgagggtct
     1321 tctttaaaac gctccataag cgtaagaatc gggtcgccag cgtaggcgtc aactttttga
     1381 aacacgcgat ggttctccag gtttacgggc aggtggttaa aacacaataa accggaagaa
     1441 ggcgaagatc gagtggatgt tcaggggcga acggcaatta gcaacagagt gagactcatg
     1501 acaaacgtac atccgccaga ggcacgacct ttataagagc atgaaagaat atcaacttat
     1561 tgaattggta ggattttatt ggccggataa ggcattcacg ccgcatccgg cacagacaat
     1621 caaatattac agaacgatta atccacgtat ttcatcgcga cccttgaagt caggcgcgta
     1681 ataagttcgt aagcgcttac tttcgtcatt tcagcgatac gttctacggg caaaccttca
     1741 ccccacaaaa tgaccgggtc cccggcttta tcctgtgcct gtggacctaa gtctacgcag
     1801 atcatatcca tcgccacgcg cccgacaatc ggcacttcgc gaccgttcac cagcactggc
     1861 gtaccggacg gcgcggcgcg cggataacca tcgccatagc ccatcgcaac tacaccaaga
     1921 cgagtatcac gttcgcttat ccaggttcca ccataaccga caggctctcc gactttatgc
     1981 tcacgcacgg caatcaggct ggaggttagt gacatcactg gctgacagcc aaaatcggca
     2041 ccggtggagc gatcttccag cggcgagacg ccataaagaa tgatgcccgg gcgcacccag
     2101 tcaaaatgcg actgtggcca cagcagaatg ccacccgacg cggcaatgga acgttgacct
     2161 ggtttgcctt cgcaaaaggt attaaagata gcgagttgtt tctcggttgc gccacatttt
     2221 ggttcatccg cgcgcgcaaa atggctgacg atattcaccg gctgccgaac gtttttgcac
     2281 tgggtcaggc gatgataaaa cgcctcagcc tgttccggcc ttacgcccag acggtgcata
     2341 ccggtatcga gtttcatcca gacggtaacc ggctcgtcca ggctagcctc ttccagcgca
     2401 gccagctgtt cttcgttatg cacggcggta tgaaaatgtt gcgcagaaat cgtcggcaga
     2461 tctctggcat caaaaaagcc ttcgagtaac agtacaggtt tggtgattcc ccccgcacgc
     2521 agtcgcagag cttcttcgag acgggctacg ccaaaggcgt cagcatcggg gagcgttcgc
     2581 gcggtctcaa gaagaccgtg accataagcg ttcgctttca ccaccgcaac cattttactg
     2641 gcaggtgcca gttcacgaag acgttgcagg ttgtgtcgca gagcgcggcg gttaatcaca
     2701 acagttgccg cttgcatttg tgttccttga taagtgtttg ctttaattac ctaattcata
     2761 aaataattat tattcgtcgt cgtactgcgg ccccgcatag ttgtcgaagc gcgaccattg
     2821 accgttaaag gtcaggcgta ccgtcccgat tgggccgtta cgttgtttac cgataataat
     2881 ttccgcgatg ccttttaaat cactgttttc gtgatacacc tcatcacgat agataaacat
     2941 gatcaagtcc gcatcctgct cgatagagcc agattcacgc aggtcggagt tgaccggacg
     3001 tttgtcggca cgttgttcca gagaacggtt caactgggac agcgccacca ccggcacgtt
     3061 cagttctttc gccagtgctt tcagcgagcg ggagatttct gcaatttcca gcgtacggtt
     3121 atcggaaagt gccggtacgc gcatcagttg caggtagtcg atcatgataa gcccgatgcc
     3181 gccgtgttca cgggcaatac ggcgtgcgcg ggaacgcact tccgttggcg tcaggccgga
     3241 ggagtcatcg atatagatat tgcgtttttc gagcaaaata cccatggtgc cggaaatgcg
     3301 cgcccagtct tcatcatcga gctgcccggt acggatttta gtctggtcaa cgcgcgacag
     3361 cgacgccaga gaacgcatca tgatctgttc tgatggcatc tccagcgaga aaataagtac
     3421 cggtttatcc tgcaacatcg ccgcgttttc gacgaggttc atcgcaaatg ttgttttacc
     3481 catcgacgga cgcgcggcga cgatgatcaa atccgacggc tgcaagccag cggttttttt
     3541 gttgagatca tcataaccgg tgtttacccc ggtaacgcca tcgtgtggct gctgaaataa
     3601 ctgctcaata cgcgccacgg ttgcgtcgag cacatcggcg atgttcttcg gcccttcgtc
     3661 tttgttcgca cgactttcgg caattttaaa gacgcgggat tcagccagat ccagcagatc
     3721 ttcgctggta cgcccctgcg gatcaaaacc agcttcggca atctcattcg caaccgagat
     3781 catctcacgg acaacggcac gttcacgcac gatgtccgca taggaactga tgttcgcagc
     3841 acttggcgta ttttttgaca actctgccag ataagcaaaa ccaccgacgc tatcgagttg
     3901 cccctggcgt tccagcgatt ccgcaagggt aatcagatcg ataggactac cgctttcctg
     3961 caaacgcgcc atttcagtaa agatatgacg gtgtgggcgg gtgtaaaaat cgtctgctac
     4021 cacacgctcg gctacatcat cccagcgttc gttatccagc attaaaccgc ccaacaccga
     4081 ctgctccgct tcgatcgagt gcggaggcac tttcagcccg gcaacttgtg gatcgcgttc
     4141 gcggggttca gcctgctgtt tgttgaaggg tttatttcct gccatagtga atggagttac
     4201 cgagataaag aatgggtcga aactttacca tatgaagcag accctgacga tacgttctgg
     4261 aggacacatg gcaacacgaa ttgaatttca caagcacggt ggcccggaag tacttcaagc
     4321 cgtagagttc actcctgccg atccggcgga gaatgaaatc caggtcgaaa ataaagccat
     4381 cggcatcaat tttatcgaca cgtatatccg cagcggcctt tacccgccgc catcactacc
     4441 cagcggatta ggtaccgaag cggcaggcat cgtgagtaaa gtcggcagtg gtgtaaagca
     4501 tattaaggca ggcgatcgtg tagtctatgc gcagtcggcg ttaggcgctt acagctctgt
     4561 gcataacatt aatgcggata aagcggcgat tctgcctgcg gcaatttctt ttgagcaagc
     4621 tgcagcatct ttcctgaaag gcttaacggt ttattatctg ctgcgcaaaa cctatgaaat
     4681 taaacccgat gaacagttcc tgttccacgc agcagctggc ggcgttggct tgattgcctg
     4741 ccagtgggca aaagccctgg gcgcgaaact tatcggcacc gtaggaaccg cgcaaaaagc
     4801 gcagagcgcg ctaaaagcgg gcgcgtggca ggttattaac tatcgtgaag agaatctggt
     4861 cgagcggtta aaagagatca ccggtggtaa aaaagtgcgc gtagtgtacg attccgtggg
     4921 cagagacacc tgggaacggt cgctggattg cctgcaacgc cgcggcttaa tggtgagttt
     4981 tggcaactca tcaggtgcgg ttaccggtgt gaacttaggc attctcaatc agaaaggctc
     5041 gttgtatgtg acacgccctt ccctgcaagg ctatatcacc acgcgggagg aattaaccga
     5101 ggccagtaat gaactgttct ctttgattgc cagcggtgtg attaaggtcg atgtcgccga
     5161 gcagcagaaa tatccgctga aggatgcgca gcgtgcgcat gagattctgg aaagccgagc
     5221 gacgcaaggt tccagcctgt tgattccata aaagaaatag ggcttccacc tgggaagccc
     5281 tttcttttta tagttcggct gtatgtaggg tacagcacga tgaatctgtt agaggcgcaa
     5341 tagtgacaga tttgattatc aattcctatt ttgttctaag gataaaacct taggttgtga
     5401 tcatccgcac aatcccttag taacgccagc ggtcataacg ctgatatttc ggcacttttg
     5461 gtgctttaat cgccttaata acccacacca ccgcaatcgc caggagtaac cacggcagca
     5521 acttaatcat caatgccagc ataccgccga ggaacataat ggccgtcgcc acaaccagcg
     5581 cggcgataat gcccagcaac gaaacgccgg tgaccatcaa catgacaaaa aagccaatca
     5641 caaaaagtag ttccagcatg atgctctccc aaatatgaaa tctcttgcag gcattacaag
     5701 aatcatgcca aaaataatct attgatttaa cagcaaaacg ccccgcgacg gtgcgcaggg
     5761 cgtggtgaat ttgactactt tttggtgaaa agttaacgct tatccgccac cagtttgagc
     5821 gcgtgttcca gcacgttaat gtctgcaccc gctttatggg cattttcact taaataacgc
     5881 cgccactgcc gcgcgccagg aataccctgg aacaagccca acatatgccg ggtaatatgg
     5941 ccaagatacg tcccctggct gagttcacgc tcaatgtacg gatacatggc gcgcactacc
     6001 gccaccggat cggcatcggt atccgaggaa ccaaagatct ctcggtctac cgccgccaga
     6061 atacccggat tctgatacgc ttcgcgcccg accatcacgc catccatatg ttgcaaatgc
     6121 gctttagcct cttccagcga cttgatacca ccgttaatcg acatcgtcag atgcggaaag
     6181 tcacgcttca gttgatacac acgcggataa tcgagcggcg ggatttcacg gttttctttc
     6241 gggcttaacc ccgaaagcca ggctttacgt gcgtggatga tgaacatctc acactcgcct
     6301 ttgccggaaa cggtgttgat gaaatcgcag agaaattcat agctgtcctg gtcatcgatg
     6361 ccaatacgcg ttttcaccgt caccggaatc gacaccacat cgcgcatcgc tttcacgcag
     6421 tcggcaacca gctgcgcatt acccatcaga cacgcaccaa acatgccgtt ctgcacccgg
     6481 tcagacgggc agccgacatt caggttgatc tcatcatatc cacgcgcttc tgccagcttc
     6541 gcacactgcg ccagcgccgc cggatcgcta ccgccgagtt gcaacgctac cggatgttct
     6601 tcttcactgt acgccaggta atcaccttta ccgtgaataa tcgcccctgt agtcaccatt
     6661 tcggtataca gcaacgtatt gcgggaaagc agacgcaaga aatagcggca atgtctgtcc
     6721 gtccagtcga gcattggtgc aacgctaaac cgagaattcc agtaaacacc agttttttca
     6781 ggcatcacgc tggtttgatt aattttttgt gcttcatgat tatcgtgcat ttttgaacat
     6841 ttcaggctat ttttctcgcg ttaggttccc gcacaggttc ccacgtttta tgggaacccg
     6901 aaataacgag gtcgtgtaat ggcgtactat aacatagaga aacgactaaa atccgatggc
     6961 acaccacgct atcgctgtaa tgtgattatc aaagaaaaag gtgttatcac ttacagggaa
     7021 agcaaaacat tccctaaaca tgctcatgcc aaaacatggg gcacacagaa agtgatggaa
     7081 ttagatctat atggcattcc atcatcaaat gcagttgacg gacttacagt ccgtgactta
     7141 ctacacaaat atttaaatga cccaaatgcc ggaggtaaag caggccgtac taaaagatat
     7201 gtgctggaac tgcttatgga tagtgacata tccgcgatca aactatctga actgacagaa
     7261 aatgacgtaa ttgaacattg caggctaaga aacaacgctg gtgcaggccc agcaacagtc
     7321 agccacgatg ttagttatct tggcagtgtt ctggatgcgg caaaacctgt atacggaatc
     7381 aattacacat caaacccggc gaaaagcgct cgtccatatc tacttaaact cggtttgatt
     7441 ggtaaatcaa accgtcgtaa tcgtagacca gcatctgatg aacttgacat gctcattgaa
     7501 ggccttcaac aacgatctac tcataaatgc tcaaaaattc cgttcgttga tatcctcaaa
     7561 ttttctgtgt ggtcctgtat gcgaatcgga gaagtatgcc ggttacgatg ggaagatctc
     7621 gaccaagaac aaaaatctat actagtaaga gataggaaag atccacgtaa aaaggaaggt
     7681 aaccatatga aagttgcctt gcttggggaa gcctgggata tcgtccagcg acaaccaaaa
     7741 aaatcagaat tcatttttcc atataacagc acttctgtta ccgcaggatt tcagagggta
     7801 agaagcaaat taggtattaa agatctgcga tatcatgatt tgcgtagaga aggggcaagt
     7861 cgcttatttg aggctggttt tagtattgag gaagtcgctc aagttacagg gcatcgttca
     7921 ttaaacgtgc tatggcaggt atataccgaa ctgtatccga aatctttaca taatcgtttt
     7981 gaagaactcc aaaagagcag aaacaagacc tcttgacact gtttatttat acagataaaa
     8041 ataacactgt atacaaacac agtatagagg gatttttatg cgtattgaaa tctgcatagc
     8101 caaagagaaa atgactaaaa tgccaaacgg tgctgtggat gcgttaaagg aagaattaac
     8161 tcggcgcatc agtaagcgtt atgacgatgt agaagtgatc gtaaaagcca ccagcaatga
     8221 tgggctttct gttacacgca ctgcagataa ggattctgca aaaacatttg ttcaggagac
     8281 tctgaaagat acctggaagt ctgctgacga gtggtttgtt cactaattag cacgtaaatc
     8341 ggtaacggct ggaaatcatc caagactagc actatcgaaa gttcaccagc caaccgcagt
     8401 acgttcttgc atacgaggtg ccgcgtttta catgccgtac cgagaattaa tttcatttta
     8461 cgaaaagtta acccgccatt gctaccttaa ttttttaaaa cgaagttacc tctgtcaggg
     8521 tcaaaagcac aattatctct tcctacaatc attgaaactg cgattgattc cctgctcaaa
     8581 ggatgagccg cccttgtttt aacaagatgt attaatgcca ttttatcata caacatacaa
     8641 acatttgaat tcattgaatt cctcataaat accccatctt ctggttcaca taatgtaaca
     8701 gggcatgttc gtaagctctc tggcacttgc aattgtgcat gctccacatt aaatgcacat
     8761 acttcaattt tatctaacaa ctcctctaac tggtagttag aaagcaattc catattttcg
     8821 agcatctcaa tttcacgcag tactgtaact tcacggcttg tcaaactacc atgattaagt
     8881 gtacgttcaa tatgttctat atattgaagg ccaatctgga tgttatcatt cgataaacca
     8941 tcctggttaa gacttcccat aaaatatcca tttacctgca taggtcg
//
LOCUS       DAZKIP010000157         8951 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZKIP010000157 DAZKIP010000000
VERSION     DAZKIP010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.158"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            35..2221
                     /gene="espL4"
                     /locus_tag="ACHOUK_004167"
     CDS             35..2221
                     /gene="espL4"
                     /locus_tag="ACHOUK_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
                     /protein_id="HFP9899505.1"
                     /translation="MITHIPRSSFSANINNTAQTNEHQTLSELFYKELEDKFSGKELA
                     TPLLKSFSENCRHNGRHIFSNKDFVIKFSISVLQADKKEITIINKNENTTLTQTIAPI
                     FEEYLMEILPQRSDALDKKELNLNSDRKEKEFPRVKLNGQCYFPGRPQNRIVCRHIAA
                     QYINDIYQNVDYKPHQDDYSSAEKFLTHFNKKCKNQTLALISSRPEGRCVAACGDFGL
                     VMKAYFDKMESNDLSVMAAILLVDNHALTVRLRIKNTTEGCIHYVVSVYDPNVTNDKI
                     RIMSESKEDIKHYSLMDFMNVDYSLLKWSNDHVINQSVAIIPALPKEQLLMLKGTVDE
                     ITPPLSPATMNLLMAIGQNHQLKQLMIQLQKMPELHRTEMLTAYNSINLPGLYLAINY
                     GNADIVETIFNSLSEPGYEGLLSKKNLMHILEAKDKNGFSGLFLAISRKDKNVVTSIL
                     NALPKLAATHHLDNEQVYKFLSAKNSTSSHVLYHVMANGDADMLKIVLDALSLLIRTC
                     HLTKEQVLDLLKAKDFYGCPGLYLAMQNGHSDIVKVILEALPSLAQEINISASDIVDL
                     LTAKSLARDTGLFMAMQRGHMNVINTIFNALPTLFNTFKFDKKNMKPLLLANNSNEYP
                     GLFSAIQHKQQNVVEMVYLALSDHARLFGFTAEDIMDFWQHKAPQKYSAFELACELGH
                     RVIAELIFNTLNKMAESFGFTDNPRYIAEKNYMEALLKKASPHTVR"
     gene            complement(2190..3926)
                     /gene="aceK"
                     /locus_tag="ACHOUK_004168"
     CDS             complement(2190..3926)
                     /gene="aceK"
                     /locus_tag="ACHOUK_004168"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFP9899506.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAEFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLHWQNKSRDIHYIIRHLTETLGPENLSKSHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(4110..5414)
                     /gene="aceA"
                     /locus_tag="ACHOUK_004169"
     CDS             complement(4110..5414)
                     /gene="aceA"
                     /locus_tag="ACHOUK_004169"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFP9899507.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAEAIHAKYPGKLLAYNCSPSFNWQKNLDDNTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(5444..7045)
                     /gene="aceB"
                     /locus_tag="ACHOUK_004170"
     CDS             complement(5444..7045)
                     /gene="aceB"
                     /locus_tag="ACHOUK_004170"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFP9899508.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGSVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEEHNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(7314..8243)
                     /gene="metA"
                     /locus_tag="ACHOUK_004171"
     CDS             complement(7314..8243)
                     /gene="metA"
                     /locus_tag="ACHOUK_004171"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFP9899509.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPEVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            8400..8843
                     /locus_tag="ACHOUK_004172"
     CDS             8400..8843
                     /locus_tag="ACHOUK_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFP9899510.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVKHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLDLAYVGE"
BASE COUNT         2341 a         2167 c         2159 g         2284 t
ORIGIN      
        1 aattcaagac atcaataaac aatgagatat ttaaatgatt actcatattc ctcgtagttc
       61 tttctctgca aatattaata atacagccca gacaaatgaa caccaaaccc tgagtgaatt
      121 gttttataaa gaactcgagg ataaattttc tggcaaggag ctggcgacac ctctattaaa
      181 aagcttctca gagaactgta gacacaatgg tcgccatatc tttagtaaca aggattttgt
      241 cattaagttt tccatatctg tcttacaagc tgataagaaa gaaattacga taattaataa
      301 aaatgaaaac acgacactca ctcaaaccat tgctccaata tttgaagaat acctaatgga
      361 aattttacct caacgctcag acgctcttga taaaaaagaa ttaaatctaa actcagatag
      421 aaaagaaaaa gaattcccaa gagttaagct taatggtcaa tgttattttc cggggcgccc
      481 ccaaaaccgt atagtatgcc gacacattgc tgcacaatat attaatgata tttatcagaa
      541 tgttgactac aaaccccatc aagatgatta ctcttcagct gaaaaatttc tcactcactt
      601 caacaaaaaa tgcaaaaacc agactttggc gttgatttcc agccgtcctg aggggcgttg
      661 cgttgctgcc tgcggtgatt tcgggctagt tatgaaggca tattttgaca agatggaatc
      721 aaatgacctc agtgttatgg ctgccatatt attggtagat aaccatgctt tgacggtccg
      781 gctaagaata aagaacacaa ctgaaggatg tatccattac gtggtttcgg tttatgatcc
      841 taatgtaact aacgataaaa taagaatcat gagcgaaagt aaagaggata ttaaacacta
      901 ttcactgatg gattttatga atgtagatta tagcctcctg aaatggtcaa atgatcatgt
      961 tattaaccaa tctgttgcaa taattccagc acttccaaaa gaacagctat tgatgctaaa
     1021 aggaactgtg gatgaaataa cccctccatt atcacctgca acgatgaatt tgctaatggc
     1081 aattggtcag aatcaccaac ttaagcaact gatgattcaa ctccagaaaa tgccagaact
     1141 acatagaaca gaaatgttga ctgcctataa tagtattaac ctccccggtt tatatttggc
     1201 tataaattat ggtaatgcgg atatcgttga gactattttt aattcattgt cagagccagg
     1261 atatgaagga ttactctcga aaaaaaatct catgcatatt ctggaggcaa aagataaaaa
     1321 tggtttttct ggattatttt tagcgatatc acgtaaggat aaaaatgttg taacctcgat
     1381 tctgaacgcc ttacctaaac tggccgcaac acatcattta gataacgaac aagtgtataa
     1441 attcctgagt gccaaaaata gtacgtccag ccatgtttta taccatgtta tggcgaatgg
     1501 tgatgccgac atgctgaaaa ttgttttgga cgcgttatct ttgttaattc gcacatgtca
     1561 tttgactaaa gaacaggtac tcgatctcct gaaggcaaag gatttttatg gttgcccagg
     1621 actatacctg gcgatgcaaa atggacatag cgatatcgtg aaagttattc ttgaagcatt
     1681 gcccagcctg gcccaggaaa ttaacatttc agcttccgat attgtagatc ttctgaccgc
     1741 taaaagtctt gcgcgcgaca cgggtttgtt tatggccatg cagcgcggac atatgaacgt
     1801 tattaatact atttttaacg cattacccac tctgtttaat acgtttaaat tcgataaaaa
     1861 aaatatgaag cccctcctcc tggcaaataa ttctaatgaa tacccaggtt tgttttcagc
     1921 gatacagcat aaacagcaaa acgttgtaga gatggtttat cttgctttat ctgaccatgc
     1981 acgcctgttt ggatttaccg ctgaagatat tatggatttt tggcaacaca aagcgccaca
     2041 aaaatactct gcctttgagt tggcttgtga attgggtcac cgggttattg ctgaattaat
     2101 ctttaataca ttaaataaga tggctgaaag ctttggcttt acggataacc ctcgatacat
     2161 tgcggagaaa aattatatgg aagctttact caaaaaagca tctccccata ccgtacgcta
     2221 aatctttgcc tgcgccgata cgcataaaca tcttccacat gcccttcacg tatgcggttt
     2281 tgtagtgcgc gccagtaatc agcgcggaac aggtcggcgt gcatctcttc aaacagcgga
     2341 ccaatacgcg ggtcggcgca tagccaatgg cgaaactctt ccgggaaaac atcgcccggc
     2401 gagacgctgt accacggttc gctggcaagt tcgtcttccg ggtagcgcgg cggcgggata
     2461 tcgcggaaat tcacttccgt catgtagcaa atttcatcgt aatcataaaa aaccacacgc
     2521 ccgtgacggg tgacaccgaa gtttttaaac agcatgtcgc ccgggaaaat gttagcagcg
     2581 gcaagctggc gaatggcatt gccgtactct tcaatggcgt cgcgcagctg ctgaccttcc
     2641 acttgttcca gccagatatt gagcggcacc atccgccgct caatataaag atggcgaatc
     2701 acaatttgtt cgccgagatc ggtgattttt tccgctgctt cctggagcag taattccatt
     2761 aatgccgggg aaatatgccg cttctccagc acaaagtttt caaactcctg ggtatccgcc
     2821 attcggccca cgcgatcgtg ctctttcacc agttgatagc aggcacgaac gtgagcggca
     2881 gacatctctt tctgcggcgc gaacttgtct ttgatgactt taaatacacg gtcaaagccc
     2941 ggcagcgtga acaccaacat caccatgcca cgaatacccg gcgcttcaat gaactgctca
     3001 ttacagccct gtagataaac gagatattcg cggtagcttt cggttttggc gtgcttctgg
     3061 cagccgatag ccatatacaa ttcagcggtg gttttacctg gcagaatttc ccgtaaccac
     3121 tcgaccagcg ctgcgggcag cggcgcataa accataaaat aagaacgcgc aaagccaaaa
     3181 acaatgctcg cttcggcggt cgtcgtcaga caggtatcaa taaacagttc gccgtcatcc
     3241 gtctggtgga tcggcaataa aaatggcaat gtgccggaag gcgttattag tttgcccacc
     3301 agccaggcgg ctttattacg gtaaaacagt tcgttcgcca cctgtaaatg acttttggag
     3361 aggttttctg gccccaacgt ttccgtcaga tggcgaataa tgtaatggat gtcacggctt
     3421 ttattctgcc agtgcagacg caacggcagg tcgctgataa cgcgcatcag cagagattcc
     3481 cagccgtgat cggggtgaaa gtctttcgcc agcgggcggg gaatggtacg aaagcggcgc
     3541 tctggctggg agctaaagat aaaaagccgc tcgggagtaa gcgagcggtg gtcaaataac
     3601 cgacagtaca cggagttaaa aaagctctcc gcaatctcga agcgcgggta atccggcaac
     3661 agccgggtgt aatgctcttt gacgcgcagt aaaaattccg cgtccgtgct ttggccgttg
     3721 gtaatgcagc gcagttgctc cacgaccaga ccaacgtgat gatcgtaaag atggatacgg
     3781 tttttcatcg cctgctggac agcatgccag tcggcttgtt cgaaacgctg ctgcgccccg
     3841 gaagtcactt cgaggaatcg accatactga gcgtcgaagc cttgcaaaat ggtttgagca
     3901 atcagtaatt ccaggccacg cggcatttag cgccctcatc aggagcagag aattgccgga
     3961 tgcggcgtaa acgccttatc cagcctacgt tcggcaacgg ttgtaggcat gataagacgc
     4021 gcaagcgtcg catcaggcat cgtgcaccga ttgccggatg cggcgtatat gccttatccg
     4081 gcctacagtc agcaacgttt gtaggttgct tagaactgcg attcttcggt ggagccagtc
     4141 agcgcggtaa ccgacgacgt gccgccctga ataatggtcg tcactttatc gaagtaacct
     4201 gtacccactt cctgctggtg agatacgaag gtgtagccat ctttcgcggc ggcgaattcc
     4261 ggctgctgca ctttctcaac atagtgcttc ataccctcgc cctgggcata ggcgtttgcc
     4321 agatcaaaca tgttgaacca catgctgtgg atacctgcca gggtgatgaa ctggaacttg
     4381 tagcccatat ccgacagctg ctgctggaag ctggcaatag tgttgtcgtc gaggtttttc
     4441 tgccagttga acgacggcga gcagttataa gccagcagtt tgcccggata tttcgcgtgg
     4501 atagcttccg caaagcgacg cgccagttcc agatccggcg tggaggtttc acaccatacc
     4561 aggtcagcat atggcgcata cgccaggcca cggctgatcg cttgctcaat gcccgcatga
     4621 gtacggaaga agccttcact ggtacgctcg ccggtaataa attcgctgtc atacgggtcg
     4681 caatcggagg tgatcagatc cgccgcatca gcatcggtac gtgcaaccag cagggttgga
     4741 acgcccgtca cgtcagctgc cagacgcgcc gcgaccagtt tctgaatagc ttcctgagtt
     4801 ggcaccaaaa ctttgccgcc catgtgaccg catttcttca ctgacgccag ctgatcttcg
     4861 aagtgaactg ccgctgcacc ggcttcaatc atcgctttca tcagttcaaa ggcattcagg
     4921 acaccgccaa aaccggcttc cgcgtcggca acgatcggca ggaagtaatc gacatagcgc
     4981 ggatcgcccg gctcaatgcc cgcggaccat tggatctgat ccgcacggcg gaaagtgttg
     5041 ttgatccgct ccaccacagc tggcaccgag tttgccggat agagcgactg atccggatac
     5101 atgctggccg ccaggttagc gtccgccgct acctgccatc ccgacagata gaccgcttca
     5161 atacccgctt tcgcctgttg cagcgcctga ccgccagtca gtgcaccgag gctgttgatg
     5221 tagccttttt tcgactcgcc atgcagcaga cgccacattt tcgccgcgcc cagttgcgcc
     5281 agcgtgcatt caggattgac tgaaccgcgt aatttcacca catcttccgc gctgtatggg
     5341 cgagtaattc cttcccaacg cggttgagtc cactctttct gtaattcttc aatttgttgt
     5401 gtacgggttt tcatgtgcag atgctccata gttatgtggt ggtttacgct aacaggcggt
     5461 agcctggcag ggtcaggaaa tcaattaact catcggaagt ggtgatctgt tccatcaagc
     5521 gtgcggcatc gtcaaaacgc ccctgggaga aacgttcttc gcccagttcg ctggcaatga
     5581 ctttcatctc ttcgcccagc atctggcgga acaaggcttt agttaccggt ttgccattgc
     5641 tcaacgtttt ttgatgatgg atccactgcc agattgaggt acgggaaatt tcagccgtcg
     5701 ccgcatcttc catcaggcca taaatcggca cgcagccgtt gccggagatc catgcttcga
     5761 tgtactgcac agccacgcga atgttggcgc gcataccttc ttcggtgcgt tcaccatcac
     5821 aaggtgccag cagctgatcg gcagtaatcg gcgcgtcttg ttcgcgcatc acttcaagct
     5881 gatttttacg ggagccgaga atgtcgttga ataccgccat tgccgtgtcc gcaaggcctg
     5941 ggtgagcgat ccatgtgcca tcgtgaccgt tattggcttc cagcgattta tccgctttta
     6001 ctttgttgag cacctggtta ttgtgctctt catctttgct cggaataaac gccgccatgc
     6061 cgcccatcgc aaaagcaccg cgtttatggc aggttttaat caacaggcgt gagtaagcat
     6121 tcaggaatgg tttatccatc gttactgcct gtctgtctgg caggacgcga tcgggatagt
     6181 ttttcaacgt tttgatatag ctgaagatgt aatcccaacg accgcagttc agaccaacaa
     6241 tatggtcacg cagcgcgtga aggatttcat ccatctggaa cacggcgggc agcgtttcaa
     6301 tcagcaacgt cgccttgatg gtgccgcgcg gcagattaaa gcgatcttct gcatagctga
     6361 agacttcgct ccaccaggcc gcttcctgcc aggactgggt tttcggcaga tagaaatagg
     6421 gaccactgcc ctttgccaac agtgcctgat agttgtggaa gaaatagagc gcaaaatcaa
     6481 acaggctgcc ggggattgcc tcaccacgcc aggtgacatg tttttccggc aagtgcagac
     6541 cgcgtacccg acaaatcaaa accgctggat tgggcttgag ctggtaaatt ttgcctgctt
     6601 cattggtgta actgatggtg ccgttaaccg catcacgcag gttaatttgc ccgtcgatca
     6661 ctttgttcca gtctggtgcc agtgaatctt cgaaatcggc cataaagact ttcacattgg
     6721 cgttgagcgc gttgatcacc atcttgcgct ctacggatcc ggtaatctct acgcggcgat
     6781 cttctaagtc cgcaggaatc ccgcgaattt tccagtcagc atcacgaatg gaagctgttt
     6841 ccgaaataaa atcaggcaac gttccgttat caatatcttg ctgctgctga atgcgcgctg
     6901 ccagaagttt attgcgttgt ggcgtaaaat gcgtcaccag ctcagtcaga aattctaccg
     6961 cttcggcagt aagaatttgc ttctcctgct cgccatacgg ccttatgaaa gccagttcat
     7021 cggttgttgt tgcctgttca gtcatcgtgc agctcctcgt catggatccg aaaacttccc
     7081 cactgtgaac gaaggatcgt tgtgcgcttt tcgttcagca caactaagac tactcattta
     7141 aaaatcaaaa tcaaaaacaa tttccatttt aattaaaaat acttgataac ttattgataa
     7201 caatatgaat aaaatttatt cacctactga ggtgtgtttc ggaaataaaa aaggcacccg
     7261 aaggtgcctg aggtaaggtg ctgaatcgct taacgatcga ctatcacaga agattaatcc
     7321 agcgttggat tcatgtgccg tagatcgtat ggcgtgatct ggtagacgta atagttgagc
     7381 cagttggtaa acagcaaatt accgtgacta cgccagctcg ctcgcggttt gttttgcgga
     7441 tcattgtgcg ggaaatagtt atacggtact tccgggtcta gtccggcttc cacatcgcgg
     7501 aaatattcct gcgccagcgt ttgcgcatca tattcgggat ggcccgtcac aaaggcaatg
     7561 cgcttatctt tactggcaaa cagatatgca tccccttctt ccgtctctgc cagaatttcc
     7621 agatcggtgt aatcacgaat caacgctgcc ggaaagtcag catagcgcga atgcggtgcc
     7681 aagaatgaat catcaaagcc acgcgtcaga agcgcatgag gatggagaat atgatgctcg
     7741 taaacgccag agagtttgtc ggtgcgagtt tgcttaggaa tgccgtagag gatattgagc
     7801 gctgcctgta ccgcccagca gacaaacagc gtcgaggtga cgtgatcttt cgaccactcc
     7861 agcacctgtt tgatctgcgg ccagtaagcg acatcattaa actccaccag gcccagcggc
     7921 gcaccggtta caatcagacc gtcaaagttc tgttcctgaa tatcttcaaa gttacagtag
     7981 aagttgttca gatgctctgc gggcgtgtta cgcgattcac gggaatcgat gcgcaacagc
     8041 tgaatatcga cctgcaaagg tgagtttgaa agcaggcgca gaaactgatt ttcagtttca
     8101 atcttcttcg gcatcaggtt aaggatcaga accttaagtg gacgaatttc ctgaccagac
     8161 gcacgagaag ttgtcatcac aaagacgttt tcttcacgca agaaattgac ggcgggtagc
     8221 tcgtccggca cacgaatcgg cataacctga ttacctcact acatacgctt atacgtttag
     8281 acatccagat agctgaagat aaccagaaaa tttgccaatg tcgagcctgc atgttgaagg
     8341 tgagaaagtt tcaaggaaaa gactacgtta gaatataaga atgactaaaa ggagagaaaa
     8401 tggttattag tattcgccgc tcacggcatg aggaagggga ggaactcgtt gcgatttggt
     8461 gtcgatctgt cgatgccacc cacgattttt tatcagcaga gtatcgggcc gagctggaag
     8521 agctggttcg ttcctttctg ccggaagcac cgttgtgggt cgcggttaat gaacgggatc
     8581 agccggttgg gtttatgttg ttaagtgggc agcatatgga tgcgctgttt atcgatcctg
     8641 atgtgcgcgg ctgcggcgtg ggccggatgc tggtgaagca tgcgctctca atggccccgg
     8701 aactgacaac caatgttaat gagcaaaatg agcaggcggt tgggttctat aagaaggtgg
     8761 gttttaaggt tacgggacgc tctgaggtgg acgatttggg gaaaccgtat ccgttgctgg
     8821 atctggcgta tgtgggggaa taagctgccc cctatcgtgc ctgcgtaagc tggataaaac
     8881 gcgccagcgt cgcatccggc atttttttgc accaacgcaa aaaggccatc cgtcaggatg
     8941 gcctttcgct t
//
LOCUS       DAZKIP010000158         8830 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZKIP010000158 DAZKIP010000000
VERSION     DAZKIP010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8830
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.159"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..1056)
                     /locus_tag="ACHOUK_004173"
     CDS             complement(<1..1056)
                     /locus_tag="ACHOUK_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899511.1"
                     /translation="MPNTSIHLSRCNILQNNKLQPTEIYKESQQTAKLEFFCDEFLKI
                     SQSRYGLSTSTDSIANLLAFFTKASDAIDSIKTQKIDVHSYGFVPIRHFVEHVITYKN
                     EFAADYEPYTLTFTRGENNEGILSVESKEGFISQRTINLNEYETAINIINEHVIKENI
                     HNTVQSPTEKYISKINSSDKPYKTSSKESIKSQLYSDQRNIQTCYYILRKNTEWYKYA
                     SSEERYDKFKNSSKEIKNTYKQIVQAQKKLNQMKYINKLSGELIDITDKKLAPLINDS
                     FSYTRDFFAYSKQEKNIFTFDNSKFVDPKEKEGLMVQHSNGQLVITGKYCPEGVQTAF
                     TQEQYDKLIRYINIFFTF"
     gene            complement(1520..2398)
                     /locus_tag="ACHOUK_004174"
     CDS             complement(1520..2398)
                     /locus_tag="ACHOUK_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9899512.1"
                     /translation="MTLTQINSLLAVLDYGGFTEASKRLFMTQSAVSQAIAALEQELG
                     VNILLRDRRKAIQLTTAGHRIVQHLRAINREVNAVKEIAEQEKQNPQRTLRLGCFPSV
                     CACILPVVIRYFEIHHPNIKIIPFEENSTAIIDSLQNESIDAGFVHFPVSGMYSVPIY
                     QDKFTVVLPPNHALAKNSTITVEELMGEPLIISKGRYELSIMALFKEKNITPQIKYEF
                     NHPDTAISFIRQGLGIALLPELTLKTIADELCSVPLEPTFYRQISLLAKEKPVEGSPL
                     FLLQMCTEQLVVSGKI"
     gene            2550..3740
                     /locus_tag="ACHOUK_004175"
     CDS             2550..3740
                     /locus_tag="ACHOUK_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9899513.1"
                     /translation="MKEKLWTKDFWAITIISFIIFFVFYVLLTLLPIYISDRLHASPD
                     KAGLLVTLFLIAAIVIRPFAGQWVGKYSNKTILVLSSLAFLVVTALYPFCHSIESLLF
                     IRVLHGITFGVITTVKGTISARLIPASRRGEGISFFSLAMGLAMVVGPWIGLNMARWE
                     AFNMAFWLCTGVAAVGIILSLIMTVPPVISHADGSKPKMGFAAMFDRAALPFAMVTFF
                     MTFSYAGVSAFLALYARELNLMSAASNFLLCYAIFLMICRTFTGNVCDKKGPKYVVYP
                     CLLFFTVGLVVLGYTQGSVMMVVSGALIGIGYGSVTPVFQTQIISSVEPHKIGVANSL
                     FFNAMDAGLALGACVMGMMVAHTGYRMIYLLGALLVVVAGGVYALQMKGKSGVALVVA
                     KEIH"
     gene            3821..4471
                     /gene="inaA"
                     /locus_tag="ACHOUK_004176"
     CDS             3821..4471
                     /gene="inaA"
                     /locus_tag="ACHOUK_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFP9899514.1"
                     /translation="MAVSAKHDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVTPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(4525..4779)
                     /gene="yfaE"
                     /locus_tag="ACHOUK_004177"
     CDS             complement(4525..4779)
                     /gene="yfaE"
                     /locus_tag="ACHOUK_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFP9899515.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4779..5909)
                     /gene="nrdB"
                     /locus_tag="ACHOUK_004178"
     CDS             complement(4779..5909)
                     /gene="nrdB"
                     /locus_tag="ACHOUK_004178"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFP9899516.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL"
     gene            complement(6136..8421)
                     /gene="nrdA"
                     /locus_tag="ACHOUK_004179"
     CDS             complement(6136..8421)
                     /gene="nrdA"
                     /locus_tag="ACHOUK_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFP9899517.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            8549..8692
                     /gene="ypaB"
                     /locus_tag="ACHOUK_004180"
     CDS             8549..8692
                     /gene="ypaB"
                     /locus_tag="ACHOUK_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFP9899518.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVINNCS
                     AIS"
BASE COUNT         2140 a         1985 c         2191 g         2514 t
ORIGIN      
        1 aaaggtaaag aaaatattaa tgtatcttat taatttatca tattgttctt gggtgaatgc
       61 tgtttgcaca ccttcagggc aatatttacc agtaataacc agttgcccat tgctgtgttg
      121 taccattaaa ccttcttttt cctttggatc tacaaactta gaattatcaa atgtgaagat
      181 atttttttcc tgttttgagt aagcaaagaa atctcgtgta tagctgaaac tatcatttat
      241 taatggtgct aactttttgt cggtgatatc gataagctca ccacttaatt tattgatgta
      301 tttcatttgg ttcaattttt tctgcgcttg tacaatttgc ttgtaggtat ttttaatttc
      361 cttagaagaa ttcttgaatt tatcatagcg ttcctcggag gatgcgtatt tgtaccattc
      421 tgtatttttt ctcagaatgt agtagcaggt ctgtatattt ctttgatcgc tgtaaagttg
      481 acttttaata ctttccttac tgctggtttt ataaggttta tcagagctgt ttatttttga
      541 aatgtatttt tctgtcggtg actggaccgt gttgtggata ttttctttta taacatgttc
      601 attgatgatg ttaattgcag tttcatattc atttaagttt attgttcttt gtgaaataaa
      661 cccttctttg ctttcgacac tcaaaatacc ttcgttgttt tcacctcggg taaaggtaag
      721 ggtatagggt tcataatccg ctgcaaattc atttttatac gttatgacat gttcgacaaa
      781 atgtcttatt ggtacgaaac cataactgtg aacgtcaatt ttttgggtct tgatactatc
      841 aattgcatct gaggctttcg tgaaaaaagc caataaatta gctatgctgt ctgttgaggt
      901 tgataacccg tatctacttt gagatatttt tagaaactca tcgcaaaaaa actcaagttt
      961 ggcggtttgt tgtgattctt tgtaaatttc tgttggttgc aatttattat tttgtaaaat
     1021 attacagcga gataagtgaa tagatgtatt tggcataaat gattccttgt attgtgagtt
     1081 aacaagatac aatttaatct ttgcagaata tattcactat caaatttagc tcattttatt
     1141 acgaagacct ttacgcatgt tagaaagtat cattattgtt aataaatacg gtaataagta
     1201 acaaacagat attgataact tccctgacaa gttattaggt atcccacttg aagattgaga
     1261 atggctccag agtaaactgt gtactgttcg ccggagtgtc attactctca acaatcggtt
     1321 ttgacagtga gtcgggcgct tcataccggt ttctacctgg agtcattgtc acttctccca
     1381 actggataaa tatatcgcca attcacaaag tgggccaaaa tcaggtgaaa acgaattatt
     1441 acgcctggta aaggctatag tacaactaag tgtggcgaat caggcagtct tgcatttgtc
     1501 atcaatctca tgtcttacat caaatttttc cactcaccac tagttgttcc gtacacattt
     1561 gtagtaaaaa cagtggactg ccttctaccg gcttttcttt agccagcaac gaaatctgtc
     1621 gatagaaggt tggttcgaga ggaacggaac acaattcatc tgcaatggtt ttcagcgtta
     1681 actctggtaa taaggcgatg cctaatccct gtcgaataaa actgatcgct gtatccgggt
     1741 gattaaattc atattttatc tgtggggtaa tattcttttc tttaaatagt gccataatgc
     1801 ttaattcata gcgtccttta ctaatgatta atggttcacc cattaattct tccactgtaa
     1861 tagtgctgtt ttttgccaga gcatggttgg gcggtaacac tacggtgaat ttatcctgat
     1921 aaatgggcac ggagtacatt ccgctcaccg gaaaatgaac aaaaccggca tcaatggatt
     1981 cattctgaag cgaatcaata atcgctgtgc tattttcctc aaaaggaata atttttatat
     2041 tcggatgatg aatttcaaag tagcgaatca caaccggtaa gatacaggcg cagacgctgg
     2101 ggaaacaacc aagtctcagc gttctttgag ggttctgctt ttcctgctcg gctatctctt
     2161 ttactgcgtt aacttcccga ttaatggcgc gcagatgttg cacaatgcgg tgacctgctg
     2221 tggtcagttg tatagcttta cggcgatcgc gtaacagtat attcacacca agttcttgtt
     2281 cgagggcagc aattgcctga cttaccgccg actgcgtcat gaacaatcgc ttgctggctt
     2341 cagtaaagcc accgtaatcc agcacggcga gtaaagaatt tatttgggta agagtcatta
     2401 gagtttgcta atcaataata ttaaaacgat taatttctct aatagcatgc cgccatttat
     2461 acttctgttc tgcaaagtac atcacaattt gacgcaaccg cagtaacggg agtgttgtct
     2521 gcgtgcgtca tcctgacagg cggtagagta tgaaagagaa gttatggacg aaggattttt
     2581 gggcaataac catcatcagc tttattattt tcttcgtctt ttatgtttta ctaacattgt
     2641 tgccaattta tatctctgac cgcttgcatg cctctcctga taaagcaggt ttgttggtga
     2701 ctttattttt aattgcggcg attgttattc gaccctttgc cgggcaatgg gtgggtaaat
     2761 attcgaataa aactattctg gtgctctctt ctctggcctt tttggtggtc actgcgctgt
     2821 atcctttttg ccactcaata gaatcactgc tttttattag ggtgcttcat ggtattacct
     2881 tcggggttat cacaacggta aagggaacga tttccgcgcg gctgatcccg gcctcccgac
     2941 gtggggaggg catcagtttt ttctctctgg caatggggct ggcaatggtg gtcgggccgt
     3001 ggattggcct gaatatggcg cgctgggagg cctttaatat ggctttctgg ttatgtactg
     3061 gcgttgcggc ggtggggatt atcctgtcgc tgattatgac cgtgccgccg gttatcagcc
     3121 atgccgacgg ttcaaagcca aagatgggct tcgccgccat gttcgatcgc gcggcattgc
     3181 cgtttgccat ggttacattc tttatgacct tttcgtatgc cggggtttct gcctttctgg
     3241 cgctttacgc ccgcgaactt aatctgatgt cggcggccag taatttcctg ctctgctacg
     3301 ccatcttcct gatgatctgc cgtaccttca ccggcaatgt ttgcgacaaa aaaggcccga
     3361 aatatgtggt ttacccctgc ctgctgttct ttacggttgg gctggtggtt ctcggctaca
     3421 cccagggcag cgtaatgatg gtcgtttctg gcgcgttgat tggtatcggg tatggttccg
     3481 tgacgccagt ttttcagacg cagattatca gttcagtgga accgcataaa atcggtgtcg
     3541 caaactccct cttcttcaat gcgatggatg caggcctggc gctgggagcc tgtgtgatgg
     3601 ggatgatggt tgcacatact ggctaccgaa tgatttatct gctgggcgca ctattagtgg
     3661 tagtggctgg tggagtctat gcgctgcaaa tgaagggaaa aagcggtgtc gcgctagtag
     3721 tggcaaaaga aattcattaa tacgacacgt ttcattaaga tttttcgcag gtaaccacct
     3781 atagtcattg tcgttaataa aaaatgacag gcgatagggt atggcagttt cagcaaagca
     3841 cgacgaattt aatcactggt gggcaacaga gggcgactgg gttgaagagc ctaactatcg
     3901 ccgtaatggt atgagcgggg tgcaatgcgt cgagcgcaac ggcaaaaagc tatatgtaaa
     3961 gcgcatgacg catcatctgt ttcattccgt acgttatcct ttcggccgac caacgattgt
     4021 ccgtgaggtt gcggtaatta aagaactgga acgggccggt gtcatcgtgc cgaagatcgt
     4081 ttttggtgaa gcggtgaaaa ttgaggggga atggcgcgcg ttgctggtga ctgaagatat
     4141 ggcggggttc atcagcattg ctgactggta tgcccagcat gcagtaacgc cctattctga
     4201 cgaagtacgg caagccatgt tgaaagcagt ggcgctggcg tttaagaaaa tgcatagcgt
     4261 taatcgtcag catggctgtt gttatgttcg ccatatctat gtgaaaacag aaggtaaggc
     4321 agaagctggt tttcttgatc tggagaaaag tcgtcgccgc ttgcgtcgcg ataaagcgat
     4381 taatcatgac ttccgccagc tggaaaaata tctggagccg atccccaaag cggactggga
     4441 gcaggtaaaa gcgtattact acgccatgta atcgttgtag gccagataat acgcgtaagc
     4501 gtcgtatctg gcgctagtac ccgatcacaa ctcgatttca atatcgcctt ttgctcggca
     4561 acaacagggc aaaatttccc ccggctgaat aaaggctaac ggttcggcaa tccagtccac
     4621 ctggcctgca accaggcgtg tgcgacagga gccgcagtaa ccttcgcggc actggtactc
     4681 aaccgccaca ttgtgggatt ccagcgccgc cagaagggaa gggtgttcat cctggcacag
     4741 cagttgtgtg ccagtgatgc gcagggtaac gcgagccatc agagctggaa gttactcaaa
     4801 tcgtcggtgt ccacttccga gtcaatctgc ccgaccagat aagaactgac ttccacttcc
     4861 tgcggagcaa cctgcacgtt atcagacacc agccaggtgt tgatccacgg gatcgggttg
     4921 gagcgcgtct ggaacggcag atccaaaccg actgcctgca tgcggatatt ggtgatgtat
     4981 tcaacgtact ggcagagaat gtctttattc agaccaatca tcgaaccgtc acggaacaga
     5041 taatccgccc agtctttctc ttgctgtgcc gcctggacaa acaggtcata gcactcctgc
     5101 ttacattctt cggcaatttc cgccatctcc ggatcatccg caccgctgcg cagcagattc
     5161 agcatatgct gagtaccggt caggtgcagg gcttcgtcgc gggcaatcag gcgaataatt
     5221 ttggcgttac cttccatcag ttcgcgttct gcaaaggcaa aggaacaagc aaagctgacg
     5281 tagaagcgga tcgcttccag cgcgttaacg ctcatcaggc agagatacag ttttttcttc
     5341 agctcgcgca ggctaacggt cacggtttta ccgttaacag tgtgggtgcc ttcgcccagc
     5401 aggtgccagt agctggtcat ttcgatcagc tcatcgtagt agctggagat cccttccgca
     5461 cgtttctgga tctgctcgtt ggtgacgata tcgtcaaaca caacagacgg atcgttaacg
     5521 atattacgaa tgatatgagt ataggaacgg gaatgaatcg tttctgagaa cgcccaggtt
     5581 tctacccagg tttccagttc cgggatcgag atcagcggca gcagtgccac gttcgggcta
     5641 cgaccctgaa tggagtccag cagcgtctga tatttcaggt tgctgataaa gatgtgtttt
     5701 tcgtgttcag gcagcgcctg gtaatcgata cggtcacgag aaacgtcaac ttcttccgga
     5761 cgccagaaga aagagagctg cttttcgatc agcttttcga agatgtcata tttttgctga
     5821 tcgtaacggg ccacgttgac cggctgacca aagaacatcg gttctttgag ctgatcattt
     5881 ttcgtctgtg aaaaggtggt atatgccatg agtgtgtcct gttgggagtt taatgccgga
     5941 taaggcattt tacgccgcat cctgtgcctg atgcgacgct ggcgcgtctt atcaggccta
     6001 caaatccgag ccgtaggccg gattaggcgt tcacgccgca tcctgtgcct gatgcgacgc
     6061 tggcgcgtct tatcaggcct acaaaccgag ccgtaggccg gataaggcgt ttacgccgca
     6121 tccggcatct caatatcaga tcttacatgc gccgctttcg cagccatcgt cctggattga
     6181 cggcaccaga tcgtcctgcg cgtcttcagc gccgtcacgg gtgttctgat aatacagcgt
     6241 tttgaccccg aatttgtagg cggtgagcag gtctttcagc aactgctgca tcggcacttt
     6301 tcctgacggg aagcgtgacg gatcgtagtt ggtgttagca gagatcgact gatcgataaa
     6361 tttctgcatg atacccacca gttgcaggta gccatcgtta cccggcattt cccacagcag
     6421 ctcataggcg tcgtgcagat gctcgtagtc cggcaccacc tggcgcagaa taccgtcttt
     6481 cgacgctttg atgctgacgt aaccgcgcgg cggttcaata ccgttagtgg cgttagagat
     6541 ctgcgaagaa gtctcggacg gcatcagagc agaaagcgtg gagttacgca ggccgtgtgt
     6601 tttgattgac tcacgcagag cttcccagtc gtaatgcagc ggttcattag cgatggcatc
     6661 cagatctttc ttataggtat cgatcggcag gatccctttc gcgtaagtgg tttcgttaaa
     6721 ccacgggcac gcgccttgct ctttcgccag ctcattagac gctttcagca ggtaatactg
     6781 aatcgcttcg aaggttttat gcgtcaggtt gttggcgctg ccgtcggagt agcgtttacc
     6841 atgcttcgcc aggtagtaag cgaagttgat cacaccaata cccagcgtac gacgacccat
     6901 cgctccacgt ttggcggccg ggatcgggta atcctgataa tccagcagcg cgtcaagtgc
     6961 acgaaccgcc agaattgcca gctcttccag ttcatccagg ctattaattg cgcccaggtt
     7021 gaaagcagac agcgtacaca gcgcgatttc accgttctcg tcgttgacgt cgttcagcgg
     7081 tttggtcggc agggctatct ccaggcacag gttagactga cgcactggcg cgatggccgg
     7141 atcaaacggg ctatgggtat tgcagtggtc aacgttctga atatagatac gaccggtaga
     7201 cgcacgttcc tgcatcatca gcgagaacag ctcaacggct ttcacacgct gcttgcggat
     7261 gctgtcgtct ttctcatatt tggtatacag gcgttcaaac tcttcctgat cggcgaagaa
     7321 tgcgtcgtac agccccggta cgtcggacgg gctgaacagg gtgatatctt cacctttcag
     7381 caggcgggta tacatcagtt tgttgatttg taccccgtag tccatatgac gcacgcggtt
     7441 gccttccaca ccacggttgt ttttcaacac cagcaggctt tccacttcca gatgccacat
     7501 cgggtagaac agcgttgccg caccgccgcg cacgccgccc tgagagcagg atttcaccgc
     7561 agtctggaaa tgtttgtaga acggaatgca gccggtatgg aacgcttcgc caccgcgaat
     7621 cgggctaccc agcgcacgaa tacgcccggc gttgatgccg atcccggcac gctgggaaac
     7681 atatttcaca atcgcgctgg aggtggcgtt gatggaatcc aggctgtcgc cgcactcgat
     7741 cagtacgcag gagctgaact gacgagtcgg ggtacgcacg ccggacatga ttggcgtcgg
     7801 cagcgaaatt ttaaatgtgg aaaccgcgtc gtaaaaacgc ttcacatatt gcaggcgcgt
     7861 ttcacgcggg tagttcgaga acaggcacgc agcaaccaga atataaagga actgggcgct
     7921 ttcgtagatt tcgccggtca cacggttctg taccagatat ttgccttcca gttgcttaac
     7981 ggcagcataa gagaaggtca tatcacggtc gtgatcgata aacgtgtcca tctgcttgaa
     8041 ctcttcttcc gtgtagtctt ccagcagatg attatcgtat ttgcccatct cgaccatttt
     8101 caccacgtgg tcgtacagcg caggcggctc aaactggccg taggcttttt tacgcaggtg
     8161 gaagatcgcc aggcgcgcgg cgagatactg ataatccggc gcatcacggg agatcaggtc
     8221 tgcggcagcc ttgataatgg tttcgtggat gtcagaggtc ttgataccgt cataaaactg
     8281 aatgtgggag cgcagctcga cctgggaaat cgaaacgtta tgcagtcctt ctgccgccca
     8341 atccagaacg cgatggattt tgtcgagatt gatgcgctct gtgctaccgt cgcgctttgt
     8401 caccagcaga ttctgattca tgtatgtcgt acctgttttt ggaattcttt ccgcaatagt
     8461 ttcgtgcaga ctacctgtag tacgggaagc tgcttagaac actatatata gggggtatgt
     8521 ttgggattca ctgcaagata gtgtgaaaat ggccctcttg caagtgcata actttgtgga
     8581 taactcagga aggaaaaagt ggctttcgcg caccttaggt cagacaaggt gtccgggaaa
     8641 gtcaatggga agagaaaaat ttgttataaa taactgttcg gcgatttctt gaacggcaga
     8701 ataggttgga taaattgata tagatggttt attaatcttt ttgctaatga ttgcaaaaat
     8761 tgatctaagc acgagtcatc acataatctg atgtatcagt tgtggtcaat atataagcga
     8821 atgtaagcga
//
LOCUS       DAZKIP010000159         8619 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZKIP010000159 DAZKIP010000000
VERSION     DAZKIP010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.161"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            213..1316
                     /gene="ompC"
                     /locus_tag="ACHOUK_004181"
     CDS             213..1316
                     /gene="ompC"
                     /locus_tag="ACHOUK_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFP9899519.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFNKNMSTY
                     VDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1428..2483
                     /gene="apbE"
                     /locus_tag="ACHOUK_004182"
     CDS             1428..2483
                     /gene="apbE"
                     /locus_tag="ACHOUK_004182"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFP9899520.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            2557..3621
                     /gene="ada"
                     /locus_tag="ACHOUK_004183"
     CDS             2557..3621
                     /gene="ada"
                     /locus_tag="ACHOUK_004183"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFP9899521.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYHKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3621..4271
                     /gene="alkB"
                     /locus_tag="ACHOUK_004184"
     CDS             3621..4271
                     /gene="alkB"
                     /locus_tag="ACHOUK_004184"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFP9899522.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            4347..5990
                     /gene="yojI"
                     /locus_tag="ACHOUK_004185"
     CDS             4347..5990
                     /gene="yojI"
                     /locus_tag="ACHOUK_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFP9899523.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPSWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELRNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            6208..7854
                     /gene="mqo"
                     /locus_tag="ACHOUK_004186"
     CDS             6208..7854
                     /gene="mqo"
                     /locus_tag="ACHOUK_004186"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFP9899524.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLNLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(8004..8492)
                     /gene="eco"
                     /locus_tag="ACHOUK_004187"
     CDS             complement(8004..8492)
                     /gene="eco"
                     /locus_tag="ACHOUK_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFP9899525.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDTGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
BASE COUNT         2078 a         2153 c         2315 g         2073 t
ORIGIN      
        1 cattccgcaa taatcttaaa aagttccctc gaatttacat tttgaaacat ctatagcgat
       61 aaatgaaaca tcttaaaagt tttagtatca tattcgtgtt ggattattct gcatttttgg
      121 ggagaatgga cttgccgact gattaatgag ggttaatcag tatgcagtgg cataaaaaag
      181 caaataaagg catataacag agggttaata acatgaaagt taaagtactg tccctcctgg
      241 tcccagctct gctggtagca ggcgcagcaa acgctgctga agtttacaac aaagacggca
      301 acaaattaga tctgtacggt aaagtagacg gcctgcacta tttctctgac gacaagtctg
      361 tagatggcga ccagacctac atgcgtcttg gcttcaaagg tgaaactcag gttactgacc
      421 agctgaccgg ttacggccag tgggaatatc agatccaggg caacagcgct gaaaacgaaa
      481 acaactcctg gacccgtgtg gcattcgcag gtctgaaatt ccaggatgta ggttctttcg
      541 actacggtcg taactacggc gtagtttacg acgtaacttc ctggaccgac gttctgccag
      601 aattcggtgg tgacacctac ggttctgaca acttcatgca gcagcgtggt aacggtttcg
      661 cgacctaccg taacactgac ttcttcggtc tggttgacgg cctgaacttt gctgttcagt
      721 accagggcaa aaacggtagc gtaagcggcg aaggcatgac taacaacggt cgtgaagcac
      781 tgcgtcagaa cggcgacggc gttggcggat ctatcactta tgattacgaa ggcttcggta
      841 tcggtgctgc agtttccagc tccaaacgta ctgatgatca gaacagcccg ctgtacatcg
      901 gtaacggcga ccgtgctgaa acctacactg gtggtctgaa atacgacgct aacaacatct
      961 atctggctgc tcagtacacc cagacctaca acgcaactcg cgtaggttcc ctgggttggg
     1021 cgaacaaagc acagaacttc gaagctgttg ctcagtacca gttcgacttc ggtctgcgtc
     1081 cgtccctggc ttacctgcag tctaaaggta aaaacctggg tgtcatcaat ggtcgtaact
     1141 acgacgacga agatatcctg aaatatgttg atgttggcgc gacctactac ttcaacaaaa
     1201 acatgtccac ctatgttgac tacaaaatca acctgctgga cgacaaccag ttcactcgtg
     1261 acgctggcat caacactgat aacatcgtag ctctgggtct ggtttaccag ttctaatctc
     1321 gattgatatc gaacaaaggg cctgcgggcc cttttttcat tgttttcagc gtacaaactc
     1381 agttttttgg tgtactcttg cgaccgttcg catgaggata atcacgtatg gaaataagct
     1441 ttacccgcgt ggcactgctg gctgccgcgc tcttctttgt tggttgcgat caaaaaccac
     1501 aacccgccaa aacccacgct actgaagtta ccgttcttga aggcaaaact atgggtacct
     1561 tctggcgtgc cagcatcccg ggcattgacg ccaaacgcag cgccgaactt aaagaaaaga
     1621 ttcagaccca gctggacgcc gacgatcagc tgctttcgac ctataaaaaa gattccgcgc
     1681 tgatgcgctt taacgactcg caaagtttat cgccgtggcc ggtaagtgaa gcgatggccg
     1741 atatcgtcac cacctcgcta cgcattggcg cgaagaccga tggcgcgatg gatataaccg
     1801 tcgggccgct ggtgaatctg tggggtttcg gtccggaaca acagccggtt caaattccga
     1861 gccaggaaca gatcgacgcg atgaaagcca aaactggttt acagcacctt acggtcatta
     1921 atcagtcgca tcagcaatat ctgcaaaaag acctgccgga tttatatgtc gatctctcca
     1981 ccgtcggcga aggttatgcg gcggatcacc tggcacgctt gatggagcag gaagggattt
     2041 cccgctatct ggtgtcggtg ggcggcgcgc tgaacagccg tggtatgaac ggtgaaggcc
     2101 tgccgtggcg ggtagcgatt caaaaaccaa ccgataaaga aaacgcggtt caggccgtgg
     2161 tggatatcaa cggtcatggg attagcacct ctggcagcta ccgtaactat tacgaactgg
     2221 acggcaaacg tctttcccat gttatcgatc cgcaaaccgg gcgtcccatc gaacacaatc
     2281 tggtatccgt gacggtgatt gcaccgacgg cgctggaagc cgacgcctgg gatacaggtt
     2341 tgatggtact cgggccggag aaagccaaag aagttgttcg ccgggaaggg ctggcggttt
     2401 atatgatcac caaagaaggc gatagcttta aaacctggat gtcaccacag tttaaaagct
     2461 tccttgtcag cgaaaaaaat taaagcgcaa gattgttggt ttttgcgtga tggtggccgg
     2521 gcagactaaa ggctatcctt aaccagggag ctgattatga aaaacgccac atgcttaact
     2581 gacgatcaac gctggcaatc tgtcttagcc cgcgacccga atgccgacgg cgaattcgtt
     2641 ttcgccgtgc gcaccacagg catcttttgc cgtccgtctt gccgcgccag acatgctttg
     2701 cgggaaaacg tctccttcta cgcaaatgcc agcgaggcac tcgccgccgg atttcgcccc
     2761 tgcaaacgct gccaacctga taaagccaat gcccagcaac atcggttgga taaaatcacc
     2821 cacgcgtgtc gactgctgga acaggaaacg cctgtaacgt tggaagcctt agccgaccag
     2881 gtggcgatga gtccatttca tctacatcgg ttgtttaaag cgactaccgg aatgacgcct
     2941 aaagcctggc aacaggcctg gcgcgctcgc cgtttgcgcg aatcgctggc gaaaggggag
     3001 agcgtgacga cgtctattct taacgccgga ttccccgaca gcagcagtta ctaccacaaa
     3061 gccgatgaaa cgctgggcat gacggctaaa caattccgtc acggtggcga aaatctggcg
     3121 gtgcgttacg cgctggctga ttgtgagctg ggtcgttgcc tggtggcaga aagcgagcgg
     3181 gggatttgcg cgatattgct gggcgatgat gacgcgacat taatcagcga gttgcagcag
     3241 atgtttcccg ctgccgacaa cgcgcctgcc gatctgacgt ttcagcaaca tgtgcgtgaa
     3301 gtgatcgcca gcctcaatca acgcgatact ccgctgacgt taccgctgga tattcgcggc
     3361 actgcttttc agcaacaagt ctggcaggca ctgcgcacga taccttgcgg tgaaaccgtc
     3421 agttatcagc aactggctaa cgccatcggc aaaccgaaag cggtacgggc cgttgccagc
     3481 gcctgtgccg ccaacaagct ggctatcgta attccctgtc atcgagtggt ccgtggtgat
     3541 ggcacacttt ccggttaccg ctggggcgtg tcgcgtaaag cgcaactgct gcgccgcgaa
     3601 gctgaaaatg aggagaggta atgttggatc tgtttgccga tgctgaaccg tggcaagagc
     3661 cactggcggc tggtgcggta attttacggc gttttgcttt taacgctgca gagcaactaa
     3721 tccgcgatat taatgacgtt gccagccagt cgccgtttcg ccagatggtc acccccgggg
     3781 gatataccat gtcggtggcg atgaccaact gtgggcatct gggctggacg acccatcggc
     3841 aaggttatct ctattcgccc attgatccgc aaacaaataa accgtggccc gccatgccgc
     3901 agagttttca taatttatgt caacgtgcgg ctacggcggc gggctatcca gatttccagc
     3961 cagatgcttg tcttatcaac cgctacgctc ctggcgcgaa actgtcgctg catcaggata
     4021 aagacgaacc ggatctgcgc gcgccaattg tttctgtttc tctgggctta cccgcgattt
     4081 ttcaatttgg tggcctgaaa cgtaatgatc cgctcaaacg tttgttgttg gaacatggcg
     4141 atgtggtggt atggggcggt gaatcgcggc tgttttatca cggtattcaa ccgttgaaag
     4201 cggggtttca tccactcacc accgactgcc gctacaacct gacattccgt caggcaggta
     4261 aaaaagaata aaaataagaa ttattattgc tgtgcgcgcg aagatgttta aactgcgggc
     4321 tgtaattcat tgtccgggtt ttctgcatgg aacttcttgt acttgtctgg cggcagtatc
     4381 gctggccatt tatcagtgtg atggcgctaa gcctcgccag tgcggcttta ggcattggct
     4441 taattgcttt tatcaatcag cgccttatcg aaacggcgga taccagtctg ctggtgttgc
     4501 cggagtttct gggattattg ctgctgttga tggcagtcac tctcggatcg caactggcgc
     4561 tcaccacttt ggggcatcac ttcgtttacc gactgcgtag tgaatttatc aagcggattc
     4621 tggatactca cgtcgagcgc attgaacaac tcggtagcgc ctcgttgctg gcgggattaa
     4681 ccagcgatgt gcgcaatatc accattgctt ttgtgcgtct gccggaactg gtgcagggga
     4741 tcattctcac tatcggttca gcggcgtatc tgtggatgct gtcgggcaaa atgttgctgg
     4801 taacggctat ctggatggca gtcaccatct ggggcggttt tgtgctggtg gcgcgggtgt
     4861 acaaacatat ggcgaccctg cgtgaaaccg aagacaagct gtacacggat tttcaaactg
     4921 tacttgaagg gcgcaaagag ctgactctga accgggaacg cgccgagtat gtgtttaaca
     4981 acctctacat tcctgatgcg caagagtatc gccatcatat tattcgcgca gacaccttcc
     5041 atcttagtgc cgtgaactgg tcaaacatca tgatgctggg cgctatcggc ctggtgttct
     5101 ggatggcgaa cagtctcggt tgggctgata ccaacgttgc cgcgacctat tcgttgacgc
     5161 ttttattcct gcgtacgccg ctgctttcgg cggttggcgc attgccgacg ctgctgacgg
     5221 cgcaggtggc gtttaacaag ctgaacaaat tcgcgctcgc gcctttcaaa gcagagtttc
     5281 cgcgcccgca ggcgtttccc agctggcaaa cgctggagct gcgtaacgtg acgtttgctt
     5341 atcaggataa cgcgttttcc gttggtccga ttaatctcac catcaaacgt ggcgagctgc
     5401 tgtttctgat tggcggcaac ggtagcggaa aatcgacgct ggcgatgttg ttgacgggct
     5461 tgtatcagcc acaaagcggc gaaatcttgc tggatggcaa acctgtcagc ggcgaacaac
     5521 cggaagatta tcgcaaactg ttttcggcag tgtttaccga tgtctggctg tttgatcaac
     5581 tgctggggcc ggagggtaaa cccgctaacc cgcaactggt tgagaagtgg ctggcgcagc
     5641 tgaaaatggc tcataagctt gagttaagaa acgggcgtat tgttaacctg aagttatcaa
     5701 aagggcagaa aaaacgcgtg gcgctgttgc tggcgctggc agaagaacgc gatattatcc
     5761 tgctggatga atgggcggcg gatcaggatc cacacttccg tcgtgagttt tatcaggtgt
     5821 tgctgccgct gatgcaggag atgggtaaaa ctattttcgc catcagtcat gatgatcatt
     5881 actttatcca cgccgaccgc ctgctggaaa tgcgcaacgg gcaacttagc gagctgacgg
     5941 gcgaagagcg cgatgccgct tcgcgtgatg ccgttgcccg gacggcataa tattacgacg
     6001 ctgacctggc ggactgctgc cgccaggtca atatgctaaa aatcccttca tttctaataa
     6061 ccctataatt aatcaacgaa attataatgt ttctaaaatt agaatataat ttataaacat
     6121 tatttaaatg ttgttactta agtgttaccg ttgatgccgc gcaaatctca cctgcaataa
     6181 agcgactaaa agtaaggcat taacaagatg aaaaaagtga ctgccatgct cttctcgatg
     6241 gccgtggggc ttaatgccgt ttcgatggcg gcaaaagcga aagcgtccga ggagcaggaa
     6301 actgatgtac tgttgattgg cggcggcatt atgagcgcca cgttggggac ctatttacgc
     6361 gagctggagc ctgaatggtc gatgaccatg gtggagcgcc tggagggtgt cgcgcaggag
     6421 agttcgaacg gctggaataa cgccggaacc gggcattctg cactgatgga actgaactac
     6481 accccgcaaa acgccgatgg cagcatcagt attgaaaaag cagtcgccat taacgaagca
     6541 tttcagattt cccgccagtt ctgggcgcac caggtcgagc gcggcgtgct gcgtactccg
     6601 cgttcattta tcaataccgt tccgcatatg agctttgtct ggggcgagga taacgtcaat
     6661 ttcctgcgcg cccgttacgc cgcgttgcaa caaagctcgc tgtttcgcgg tatgcgttac
     6721 tctgaagatc acgcgcagat caaagagtgg gcaccgttag tgatggaagg gcgcgatccg
     6781 caacagaaag tggcagccac gcgtacggaa attggtaccg atgtgaacta cggcgagatc
     6841 acccgccagt taattgcttc cttgcagaag aaatctaact tctcgctgca actcagcagc
     6901 gaagtccgcg ccctaaagcg taatgacgat aacacctgga ccgttaccgt tgccgatctg
     6961 aaaaatggca ctgcacagaa catccgtgcg aaatttgtct ttatcggcgc gggcggtgcg
     7021 gcactgaagc tgttacagga atcggggatt ccggaagcga aagactacgc cggtttcccg
     7081 gtgggcggac agttccttgt ttcggaaaac ccggacgtgg ttaatcacca tctggcgaag
     7141 gtttacggta aagcatccgt tggcgcacca ccgatgtcgg ttccgcatat cgatacccgc
     7201 gttctggacg gtaaacgcgt agtgctgttt gggccatttg ccaccttctc aaccaaattc
     7261 ctcaaaaacg gttcattgtg ggatctgatg agttccacca ccacctctaa cgtgatgccg
     7321 atgatgcacg tcgggctgga taatttcgat ctggtgaaat atctggtgag tcaggtgatg
     7381 ttgagtgaag aggatcgttt tgaagcgttg aaagagtact atccgcaagc gaaaaaagag
     7441 gactggcgtt tgtggcaagc ggggcagcgt gtgcagatta tcaagcgtga tgccgataaa
     7501 ggtggtgtac tgcgtctggg tactgaagtg gtcagtgacc agcaaggaac cattgccgcg
     7561 ctcctggggg catcgccagg ggcgtcaacc gccgcgccga ttatgttgaa tctgctggaa
     7621 aaagtatttg gcgatcgcgt ttccagcccg caatggcagg ctacgttgaa agcgatcgtt
     7681 ccatcttatg gacgcaagct gaacggtgat gtagcggcaa cagaacgcga gttgcagtac
     7741 accagcgaag tgctggggct gaaatacgac aaaccgcaag cagcagatag tacgccgaaa
     7801 ccgcagttga aaccgcaacc cgttcaaaaa gaagtggcgg atattgcgtt gtaatgatgt
     7861 gccacatccg gcatggtatg ccggatgtgg cgtatgctga taagacgcgc cagcgtcgca
     7921 tcaggcaacc ggctcgggcg ttagatgtcg gatgcggcgt gaccgcttta tccaacctac
     7981 ggggtgccgc acttcacggc agtttagcga actaccgcgt tgtcaatttt ctcttccgcc
     8041 ttccagacgc ggtacttcac atctacattg tctggcgtat acaccacgat cggcagcttg
     8101 ctgttgtaac gcagcattcc agtatcgccc agatacgcgg tgacaaattt cttctctttc
     8161 ttgccatccg ggcaggccat catcgtggaa accggcgagc taactttatc aaagacataa
     8221 taatcatagc cccagccttc cagcgttttg ctttccagct tcccgccgag acgatgcaaa
     8281 ttgcaatcga cttccagcgt ctgaccgatt aacagttcta ctttcagggt agattcatct
     8341 tcttgcgggg ttaactgaat cacctgacgc ttcatgcctt tttcagcttg tggataaggc
     8401 gcgatttttt ccagtggctg gacgctctct gccgcccagg cggaagtggt ggcgaaagcg
     8461 gcaaacaata ctgcaggtag aatggtcttc attttttgct ttccttcttc atttataggt
     8521 ttgcaccgat gatgtcgctg aaggttaaca tattcatcag atttattaat ttgctttatg
     8581 aataacttaa agaatattca gacaatttta cctgctctt
//
LOCUS       DAZKIP010000160         8575 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZKIP010000160 DAZKIP010000000
VERSION     DAZKIP010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.162"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            83..343
                     /gene="dinJ"
                     /locus_tag="ACHOUK_004188"
     CDS             83..343
                     /gene="dinJ"
                     /locus_tag="ACHOUK_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HFP9899526.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGV"
     gene            346..622
                     /gene="yafQ"
                     /locus_tag="ACHOUK_004189"
                     /pseudo
     CDS             346..622
                     /gene="yafQ"
                     /locus_tag="ACHOUK_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
     gene            778..1518
                     /gene="dpaA"
                     /locus_tag="ACHOUK_004190"
     CDS             778..1518
                     /gene="dpaA"
                     /locus_tag="ACHOUK_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFP9899527.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(1489..2256)
                     /gene="yafJ"
                     /locus_tag="ACHOUK_004191"
     CDS             complement(1489..2256)
                     /gene="yafJ"
                     /locus_tag="ACHOUK_004191"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFP9899528.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGKTDSEKAFCWLLYKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(2361..2939)
                     /gene="lpcA"
                     /locus_tag="ACHOUK_004192"
     CDS             complement(2361..2939)
                     /gene="lpcA"
                     /locus_tag="ACHOUK_004192"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFP9899529.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            3179..5623
                     /gene="fadE"
                     /locus_tag="ACHOUK_004193"
     CDS             3179..5623
                     /gene="fadE"
                     /locus_tag="ACHOUK_004193"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFP9899530.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTLGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNVFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(5666..6112)
                     /gene="ivy"
                     /locus_tag="ACHOUK_004194"
     CDS             complement(5666..6112)
                     /gene="ivy"
                     /locus_tag="ACHOUK_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFP9899531.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            6293..7063
                     /gene="yafV"
                     /locus_tag="ACHOUK_004195"
     CDS             6293..7063
                     /gene="yafV"
                     /locus_tag="ACHOUK_004195"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFP9899532.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMVALREYREKFPAWRDADEFRLR"
     gene            complement(7181..8353)
                     /locus_tag="ACHOUK_004196"
     CDS             complement(7181..8353)
                     /locus_tag="ACHOUK_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc26 family transposase"
                     /protein_id="HFP9899533.1"
                     /translation="MSIQSLLDYISVIPDIRQQGKVKHKLSDILFLTVCAVIAGADEW
                     QEIEDFGHERLEWLKKYGDFDNGIPVDDTIARVVSNIDSLAFEKIFIEWMQECHEITD
                     GEIIAIDGKTIRGSFDKGKRKGAIHMVSAFSNENGVVLGQVKTEAKSNEITAIPELLN
                     LLDLKKNLITIDAMGCQKDIASKIKDKKADYLLAVKGNQGKLHHAFEEKFPVNVFSNY
                     KGDSFSTQEISHGRKETRLHIVSNVTPEFCDFEFEWKGLKKLCVALSFRQKKEDKSAE
                     GVSIRYYISSKDMDAKEFAHAIRAHWLIEHSLHWVLDVKMNEDASRIRRGNAAEIISG
                     IKKMALNLLRDCKDIKGGVKRKRKKVALNTCYIEEVLASCSELGFRTDKMKNLTQI"
     gene            8434..>8575
                     /locus_tag="ACHOUK_004197"
     CDS             8434..>8575
                     /locus_tag="ACHOUK_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899534.1"
                     /translation="MIYITIFMILPCPVPCSHVFLYVFYIFLFLVLFIMTIYQSSQKL
                     HFS"
BASE COUNT         2087 a         2138 c         2093 g         2257 t
ORIGIN      
        1 ttaaattact catagattgt cgctgttgct catttgagct acaattcaag ctgaataaat
       61 atacagcata ggagatatcc caatggctgc taacgcattt gttcgcgccc gaatcgatga
      121 agatctgaag aatcaggcgg cggacgtact ggccgggatg gggctgacca tctctgacct
      181 ggttcgcata accctcacaa aggtcgcgcg tgaaaaggca ttgccgtttg atttacgcga
      241 gcctaatcaa ttaaccattc aatcaatcaa aaacagcgaa gctggcgttg atgttcataa
      301 ggccaaagac gccgatgatt tatttgataa attaggagtt taaatatgat tcaaagggat
      361 attgaactcg ggacaatttt caaaggatgt aaaacttgca caaaagcgtc ataaggatat
      421 gaataaattg aaatatctta tgacgcttct tatcaataat actttaccgc ttccagctgt
      481 ttataaagac cacccgctgc aaggttcatg gaaaggttat cgcgatgctc atgtcgaacc
      541 ggactggatc ctgatttaca aacttaccga taaactttta cgatttgaga gaactggaac
      601 tcacgcggcg ctctttgggt aaagcccgct gccgatgaaa tttgtaaacc ctgcgccgac
      661 aggcttgcgt aaaactcttt tggtgataat gtgagccttc ccaatccaga ctattccgat
      721 ttgggatcca ttttgccgtc ctggcgtgtg taaccgtttt atcaaggaat aagcagtatg
      781 cgtaaaatcg cattaattct tgcgatgttg ttgatcccgt gcgtttcgtt tgccggtttg
      841 ctgggcagca gtagctcgac cacgccagtg agcaaagagt ataagcagca gttgatggga
      901 tcccctgtct acatccagat cttcaaggaa gaacgtacgc tcgatctcta cgtcaaaatg
      961 ggcgagcaat atcaactgct cgacagctat aaaatctgta aatattccgg cggcttaggg
     1021 ccaaaacagc gtcagggcga tttcaaaagc ccggaagggt tttatagcgt ccagcgtaat
     1081 cagttaaaac cagacagccg ttactacaaa gcgattaata ttggtttccc caatgcctat
     1141 gaccgtgcgc atggttatga agggaaatac ctgatgatcc acggcgattg tgtttccatc
     1201 ggctgctacg cgatgaccaa tcagggcatt gacgagattt tccagttcgt tactggcgca
     1261 ctggtatttg gtcagccgag cgtgcaagtg agcatttacc cgttccgcat gaccgacgcc
     1321 aatatgaagc gccataaata ttccaacttt aaggacttct gggagcaact gaagccaggc
     1381 tacgactact ttgagcaaac ccgtaagcca ccgaccgttt ctgtggtcaa tggccgttac
     1441 gtggtcagca agccgttaag ccacgaagtt gtgcaaccac aactggcatc aaactacacg
     1501 ctccccgagg caaaataagc gccattcgcc tggcataatc ttttgccagg tttcattgcc
     1561 cgtcagcggc tgtgtcgcaa tcaccgtgac cacatcattc ggtgtggtct gcgagctgaa
     1621 gtcgatttcc acatcctgat ccagcaacgt tgccacgcca aacggcgcgc ggcgggtgat
     1681 ccagtgcaga ttggtcgagc aatacgccat tacatagcgc ccatccgaaa gcagcatgtt
     1741 gaaaacgccc ttctgccgca gttcatccgc cagtgaggcg atatatttaa ataccgccgc
     1801 catattgccc ggcgtacgcg gataacgctg cgttaattta tacagcagcc agcaaaaggc
     1861 tttttcgctg tcggttttac ctaccgggcg gaagttgccg gtttccagtg atttatagcc
     1921 cgtcagttgt ccgttatggg cataagtcca gttacgtccc cataactcgc gggtaaacgg
     1981 gtgagtattt tccaacgcca cctcgccccg atttgcctgg cgaatatgag ccacaaccga
     2041 acaggatttt atcgggtagt cctgaacaag tttggcaatg ggggaattaa agctgggttg
     2101 tggatcttta aatgtgcgac agcctttacc ttcgtaaaag gtaatgcccc agccatcttt
     2161 atgtggcccg gttccaccac cacgctgcac cagcccggtg aaactaaagc aaatatcggt
     2221 agggacgttg gcgctcatcc cgagcaattc gcacatcggt aacccccaca acagcgggag
     2281 cgcatggccc ccggcgaaga ttgacttaag aattttcagg ccggatgcgg cgtaaacgca
     2341 ttattcggcc tacgccagac ttacttaacc atctcttttt caattaactg gatcaggata
     2401 tggatcactt taatgtgaat ttcctgaatg cggtcggcat aaccaaaatg tggaacgcga
     2461 atttcaatat ccgccgtacc agccattttg ccgccatctt tgccggtcag ggtaatcact
     2521 ttcattccct tctcacgcgc cgccgcgatc gctttgatca cgtttgcaga gttaccagac
     2581 gtggagatcc ccagcagtac atcgccttcg cggcctaccg cttcaacata gcgggagaaa
     2641 atatcattga aaccaaaatc gttaccgacg caggaaatat ggctgacgtc agagatggca
     2701 atcgccgggt agcccggacg gttttcacgg tagcgaccgg tcagctcttc ggcaaagtgc
     2761 atggcgtcgc aatgggagcc gccgttgccg caggaaagca ccttgccacc ggctttaaag
     2821 ctgtctgcta acaggaccgc cgctcgctga atggcgtgaa tattggcgtc atcttttaaa
     2881 aagttagcca gcgtttccgc cgcttcgttc agttcgttac gaataagatc ctggtacatg
     2941 aggatatcct tcagcataaa tgtaatagac aaaatgcagt gtaccggata ccgccaaaag
     3001 cgagaagtac gggcaggtgc tatgaccagg actttttgcc ctgaagtgta gataaaaaca
     3061 gcaacaatgt gagcttcgtt gtaattatat tgtaaacaca ttgctaaatg tttttacatc
     3121 cactacaacc atatcatcac aagtggtcag acctcctaca agtaaggggc ttttcgttat
     3181 gatgattttg agtattctcg ctacagttgt cctgctcggc gcgttgttct atcaccgcgt
     3241 gagcttattt atcagcagtc tgattttgct cgcctggaca gctgccctcg gcgtcgctgg
     3301 tctgtggtcg gcgtgggtac tggtgcctct ggccattatc ctcgtgccgt ttaactttgc
     3361 acctatgcgt aagtcgatga tttccgcgcc ggtatttcgc ggtttccgta aggtgatgcc
     3421 gccgatgtcg cgcactgaga aagaagcgat tgatgcgggt accacctggt gggaaggcga
     3481 cttgttccag ggcaagccgg actggaaaaa gctgcataac tatccgcagc cgcgcctgac
     3541 cgcagaagag caagcgtttc tcgacggccc ggtagaagaa gcctgccgga tggcgaatga
     3601 tttccagatc acccatgaac tggcggatct gccgccggag ttgtgggcgt atctgaaaga
     3661 gcatcgcttc ttcgcgatga tcatcaaaaa agagtacggc ggcctggagt tttcagctta
     3721 tgcccagtct cgtgtgctgc aaaaactctc cggcgtcagc gggatcctgg cgattaccgt
     3781 cggcgtgccg aactcattag gccccggcga gctgctgcaa cattacggca ctgacgagca
     3841 gaaagatcac tatctgccgc gtctggcgcg tggtcaggag atcccttgct ttgcactgac
     3901 cagcccggaa gcgggttccg atgcgggcgc gattccggac accgggattg tctgcatggg
     3961 cgaatggcag ggccagcagg tgctggggat gcgtctgacc tggaacaaac gctacattac
     4021 gctggcaccg attgcgactg tgcttgggct ggcgtttaaa ctctccgacc cggagaaatt
     4081 actcggcggt gcagaagatt taggtattac ctgtgcgctg atcccgacca ccacgccggg
     4141 cgtggaaatt ggtcgtcgcc acttcccgct gaatgtgccg ttccagaacg gaccgacgct
     4201 cggtaaagat gtcttcgtac cgatcgacta cattatcggc gggccgaaaa tggccgggca
     4261 aggctggcgg atgctggtgg agtgcctctc ggtaggccgc ggcatcaccc tgccttccaa
     4321 ctcaaccggc ggcgtgaaat cggtagcgct ggcaacgggc gcgtatgctc acattcgccg
     4381 tcagttcaaa atctctatcg gtaagatgga agggattgaa gagccgctgg cgcgtattgc
     4441 cgggaacgct tatgtaatgg acgctgcggc atcgctgatt acctacggca ttatgctcgg
     4501 cgaaaaacct gccgtgctgt cggctatcgt taagtatcac tgtacccacc gcgggcagca
     4561 gtcgattatt gatgcgatgg atattaccgg cggtaaaggc attatgctcg ggcaaagcaa
     4621 cttcctggcg cgtgcttacc agggcgcacc gattgccatc accgttgaag gggctaacat
     4681 tctgacccgc agcatgatga tcttcggaca aggagcgatt cgttgccatc cgtacgtgct
     4741 ggaagagatg gaagcggcga agaacaatga cgtcaacgtg ttcgataaac tgttgttcaa
     4801 acatatcggt cacgtcggta gcaacaaagt tcgcagcttc tggctgggcc tgacgcgcgg
     4861 tttaaccagc agcacgccaa ccggcgatgc cactaaacgc tactatcagc acctgaaccg
     4921 cctgagcgcc aacctcgccc tgctttctga tgtctcgatg gcagtgctgg gcggcagcct
     4981 gaaacgtcgt gagcgtatct cggcacgtct gggtgatatt ttaagccagc tttacctcgc
     5041 ctctgccgtt ctgaagcgtt atgacgacga aggccgtaat gaagccgatc tgccgctggt
     5101 gcactggggc gtacaggatg cgctgtatca ggcagaacag gcgatggacg atttactgca
     5161 aaacttcccg aaccgcgtgg ttgccgggct gctgaatgtg gtgatcttcc cgaccggacg
     5221 tcattatctg gcaccgtctg acaagctgga tcataaagtg gcgaagattt tacaagtgcc
     5281 gaacgccact cgctcacgta ttggtcgcgg tcagtacctg acgccaagcg agcataaccc
     5341 ggttggttta ctggaagagg cgttggtgga tgtgattgcc gccgacccga ttcatcagcg
     5401 gatctgtaaa gagctcggta aaaatctgcc atttacccgt ctggatgaac tggcgcacaa
     5461 cgcgctggcg aaggggctga ttgataaaga tgaagccgct attctggtga aagcggaaga
     5521 aagccgtctg cgcagtatta acgttgatga ctttgatccg gaagagctgg cgacgaagcc
     5581 ggtaaagttg ccggagaaag tgcggaaagt tgaagccgcg taattgaata acggagccga
     5641 aaggctccgt ttctttatcc gctaattatt taaaattaaa gccatccgga tggttttcca
     5701 ggctgccggt caacgccgcg aacagcaccg ttttaccatc aatcgaaagc gcatcgttca
     5761 cattcaacca ggtcagtttc tcttgcgacg ttttctcatc aatagtcgag aacagccccg
     5821 tcatctgatt agatttctcg gaccacatca cagcgatacg ttgcgagcca cagtcatgcg
     5881 gtttgcacgc gctcatcacc tgatacgtct catctcccaa cgttacggtt tgtgcagggg
     5941 tataagtacc gcctttcatc acccaggcag gcagcttatg cccttgcacc atctgattaa
     6001 atgcagcttt ggtggtttcg ccctttgcaa ggctgctaat ggttaaatca tcctgcgcca
     6061 ttgcactggt ggcgatgacc agtgcggcga ctgtcgttat tgccttaaac atcattcctc
     6121 ccgagcttat cctgcccatg ttattaacct cctaaagcct ggcgataaca cgataatttg
     6181 caaatcagat atttccaata tcagcatttg ttatcaattt attctgcgct aaggcaactg
     6241 cctgataagg tgttaaagtt ataaaaccct tcgcataaag gagttgtaca ccgtgcctgg
     6301 tttgaagatt acgcttttgc agcaaccact ggtgtggatg gatggtcctg ccaacctgcg
     6361 tcattttgat cgtcaactgg aaggtattac cgggcgcgat gtgatcgttc taccggagat
     6421 gtttaccagc ggctttgcca tggaagcggc tgcttcgtcg ctagcacaag atgacgtagt
     6481 gaactggatg acagccaaag cgcagcagtg caatgcgctg attgcaggca gtgttgcatt
     6541 acaaacggag tctggttcgg ttaaccgctt tttgctggtt gagccgggcg gcacggtaca
     6601 tttttatgat aagcgtcatc tgttccgcat ggcagatgag catctgcatt ataaagcggg
     6661 caatgcgcga gtgattgtgg aatggcgcgg ctggcgtatt ttgccgttgg tgtgctacga
     6721 cttacgtttt ccggtgtggt cgcgcaatct caacgattat gacctcgcca tctacgttgc
     6781 caactggcct gctccgcgct ctctgcactg gcaggcattg ctgacggccc gcgcgattga
     6841 gaatcaggcg tatgtggcgg gatgcaatcg cgtcggcagc gatggcaacg gctgccatta
     6901 tcgcggtgac agccgggtga ttaatccaca aggcgagatt atcgctactg ccgacgcgca
     6961 tcaggcaacg cgcattgatg cggagctgtc gatggtggca ttgcgggaat atagagaaaa
     7021 gtttccggca tggcgggatg cggatgagtt taggttgcgg tgagtggggg ctgtatttag
     7081 ggcggggaat tcagaaaggg atgtttagtg tgctgagcga gagttaaata ataaaaaact
     7141 gggaagattt aataatcccc ccgcagggca agagcatgaa ttaaatctgt gttaaatttt
     7201 tcattttgtc agttcgaaac ccaagctctg agcaggatgc aagcacttct tctatataac
     7261 atgtgtttaa cgcaaccttc tttcttttcc tcttcacccc acccttaatg tctttgcaat
     7321 ctcttaataa attcagtgcc atcttcttta ttccagatat tatttcggct gcgtttcctc
     7381 ttcttatccg gctggcatct tcattcattt ttacatctaa cacccaatga agactgtgct
     7441 cgatcagcca gtgcgctctg atagcatgtg caaattcttt agcatccata tcctttgatg
     7501 aaatataata tcggatgctt acaccttctg ctgatttatc ttctttcttc tgcctgaatg
     7561 acaatgctac acaaagcttt tttaatccct tccattcgaa ttcaaaatca caaaattcag
     7621 gcgttacgtt actgacaata tgcaaacgtg tttcttttct tccatgactt atctcctgcg
     7681 tactaaacga atcgccttta taattagaaa acacatttac aggaaatttt tcctcgaatg
     7741 catgatgtaa tttcccctga ttgcctttta ctgccagaag ataatctgct tttttatctt
     7801 tgatcttcga agcgatatct ttctgacagc ccatagcatc aatggttatc aaatttttct
     7861 ttaaatccag taggttaagc aactctggaa tggctgtaat ctcattactt ttggcttccg
     7921 tttttacctg ccccagtaca acaccatttt cgttcgagaa tgcactcacc atatggattg
     7981 ctccttttct ttttccctta tcaaaggagc ctcttatggt ctttccatct attgctataa
     8041 tttcaccatc agtgatttca tggcactcct gcatccattc aataaatatc ttttcaaagg
     8101 ccaaactgtc aatgttactc acaacgcgtg caatggtgtc atcgaccgga atgccattat
     8161 caaaatcacc atatttcttt agccattcaa gtctttcatg tccaaaatct tcaatttcct
     8221 gccactcatc ggcacctgca attactgcac atacggtgag aaacaaaata tcagataatt
     8281 tatgtttaac ctttccttgt tgtcgtatat cagggatcac tgaaatataa tcaagcaaac
     8341 tttgaatact cacctggcgt cctttttatc tttttagtta ttccaaaggt taaattttga
     8401 tcacctgtag gcgttaaagc caggatgaaa atcgtgattt acatcacgat tttcatgatc
     8461 ttgccctgcc ccgtcccttg ctctcatgtc tttctgtatg tattttatat ttttctcttt
     8521 ctggtgttgt tcattatgac catttaccaa tccagtcaaa aacttcattt ttcaa
//
LOCUS       DAZKIP010000161         8467 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZKIP010000161 DAZKIP010000000
VERSION     DAZKIP010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.163"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..203)
                     /locus_tag="ACHOUK_004198"
     CDS             complement(<1..203)
                     /locus_tag="ACHOUK_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9899535.1"
                     /translation="MNQHNTRLIRLPEVLHRTGYGKAWIYHLINQNRFPSPVKIGARS
                     VAFIESEVDEWIQLTIENSRKHVA"
     gene            complement(303..1154)
                     /locus_tag="ACHOUK_004199"
     CDS             complement(303..1154)
                     /locus_tag="ACHOUK_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6387 family protein"
                     /protein_id="HFP9899536.1"
                     /translation="MKNWSVEHTREVKKWLDIDTYRGFEELPLIHLYHELLARTLFFK
                     PYHEEFEAEAVRLYIDRIFTGKPFLITEKHLGYLTREDTLYQPPHFFLTTTERLAQLS
                     IVGLRNSLFFWDGSDEYSVNREFLDSPVSEVLPKLFRDTVMVEIDLANGTDEEIAESL
                     KAALPQWRKVRGIEPDVTEAIRFGYGTIKKIINYRLLPMIDILVWSKLYKVRISEDRL
                     SRLLYTDDDDEINTRLNHQIRDTDKPLALKAASIPFIRQFNLFINKNSHLKKIRVSDV
                     MKIADSD"
     gene            complement(1581..6167)
                     /gene="ypjA"
                     /locus_tag="ACHOUK_004200"
     CDS             complement(1581..6167)
                     /gene="ypjA"
                     /locus_tag="ACHOUK_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin-like autotransporter YpjA/EhaD"
                     /protein_id="HFP9899537.1"
                     /translation="MNRTSPYYCRRSVLSLLISALIYAPPGMAAFTPDVIGVVNDETV
                     DGSQRVDERGTTNNTHIINHGQQNVYGGVSNGSLIESGGYQDVGRHNNYVGQSNNTTI
                     NGGRQSIHDGGISTGTIIESGNQDVYKGGISNGTTIKGGASRVEGGSANGTLIDGGSQ
                     IVKVQGHADGTTINKSGSQDVVQGSLATNTTINGGRQYVEQSTVETTTIKNGGEQRVY
                     ESRALDTTIEGGTQSLNSKSTAKNTQIYSGGTQIIDNTSSSDVIEVYSGGVLDVSGGT
                     ATNVTQHDGAILKTNTNGTTVSGTNSEGAFSIHNHVADNVLLENGGHLDINAYGSANK
                     TIIKDKGTMSVLTNAKADATRIDNGGVMDVAGNATNTIINGGTQNINNYGIATGTNIN
                     SGTQNIKSGGKADTTIISSGSRQVVEKDGTAIGSNISAGGSLIVYTGGIAHGVNQETG
                     SALVANTGAGTDIEGYNKLSHFTITGGEANYVVLENTGELTVVAKTSAKNTTIDAGGK
                     LIVQKEAKTDSTRLNNGGVLEVQDGGEAKHVEQQSGGALIASTTSGTLIEGTNSYGDA
                     FYIRNSEAKNVVLENAGSLTVVTGSRAVDTIINANGKMDVYGKDVGTVLNSAGTQTIY
                     ASATSDKANIKGGKQTVYGLATEANIESGEQIVDGGSTEKTHINGGTQTVQNYGKAIN
                     TDIVSGLQQIMANGTAEGSIINGGSQIVNEGGLAENSVLNDGGTLDVREKGSATGIQQ
                     SSQGALVATTRATRVTGTRADGVAFSIEQGAANNILLANGGVLTVESDTSSDKTQVNT
                     GGREIVKTKATATGTTLTGGEQIVEGVANETTINDGGIQTVSANGEAIKTTINEGGTL
                     TVNDNGKATDIVQNSGAALQTSTANGIEISGTHQYGTFSISGNLATNMLLENGGNLLV
                     LAGTEARDSTVGKGGAMQNQGQDSATKVNSGGQYTLGRSKDEFQALARAEDLQVAGGT
                     AIVYAGTLADASVSGATGSLSLMTPRDNVTPVKLEGAIRITDSATLTIGNGVDTTLAD
                     LTAASRGSVWLNSNNSCAGTSNCEYRVNSLLLNDGNVYLSAQTAAPATTNGIYNTLTT
                     NELSGSGNFYLHTNVAGSRGDQLVVNNNATGNFKIFVQDTGVSPQSDDAMTLVKTGGG
                     DASFSLGNTGGFVDLGTYEYVLKSDGNSNWNLTNDVKPNPDPNPNPNPNPKPDPKPDP
                     KPDPKPDPTPEPTPTPVPEKRITPSTAAVLNMAATLPLVFDAELNSIRERLNIMKASP
                     HNNNVWGATYNTRNNVTTDAGAGFEQTLTGMTVGIDSPNDIPEGIATLGAFMGYSHSH
                     IGFDRGGHGSVGSYSLGGYASWEHESGFYLDGVVKLNRFESNVAGKMSSGGAANGSYH
                     SNGLGGHIETGMRFTDGNWNLTPYASLTGFTADNPEYHLSNGMESKSVDTRSIYRELG
                     ATLSYNMRLGNGMEIEPWLKAAVRKEFVDDNRVKVNNDGNFVNDLSGRRGIYQAGIKA
                     SFSSTLSGHLGVGYSHGAGVESPWNAVAGVNWSF"
     gene            complement(6232..6351)
                     /locus_tag="ACHOUK_004201"
     CDS             complement(6232..6351)
                     /locus_tag="ACHOUK_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFP9899538.1"
                     /translation="MPHSYAEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            complement(6575..6733)
                     /locus_tag="ACHOUK_004202"
                     /pseudo
     CDS             complement(6575..6733)
                     /locus_tag="ACHOUK_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(7103..8452)
                     /locus_tag="ACHOUK_004203"
     CDS             complement(7103..8452)
                     /locus_tag="ACHOUK_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
                     /protein_id="HFP9899539.1"
                     /translation="MLINKSNGFNASAVWGSGSYNENKSSKHMELLAHSIVKLICKEA
                     ASETYRGALEILQKIMSECIYQEGNAFVIMGAGEQLKRIKYDVDENNLKVFNVHFDNN
                     EVLVTDGEPDVVCLSKQVWENLLIKLKPEIKENVASEVHKSANKGEIEQLVEWSKRNE
                     QTLFDNIIKSDFHVGLLKPGSMNGVILEMPPNVCMEPRNSYENKIDEVSSLSESEEHP
                     IDIQKITDAFVKEFKGILFDKNGRSSELLFNFYECCYTFLPRAQPQDKIDSYNSALQA
                     FSIFCSSTLTHNNVGFDFKLFPEVKLSGEHLETVFKYKNGDDVREIAKINITLQKEEG
                     GLYNLRGLDFKGCFFSGQNFSNYDIQYVNWGMSLFDVDTPCIFNTPANHESYEKSLKP
                     VSENGLNGVLSDRNKKIKMITGVAPFDDILFMDDDFDDNSPEDAPIENSPVVNSPLV"
BASE COUNT         2155 a         2067 c         1737 g         2508 t
ORIGIN      
        1 gcaacatgct ttcttgaatt ttcgatagtt aattgaatcc attcgtctac ttcactttca
       61 atgaaagcaa cagagcgcgc accgatttta acgggggaag gaaaacgatt ttggttgatg
      121 aggtgataga tccaagcttt accgtatcca gttcgatgca gaacctctgg taaacgaata
      181 aggcgggtgt tgtgctgatt catagtcgtt tcctctgtgc cgttttgatg gaaatgacta
      241 tacaaatgaa tcagcaggaa aaagaccgaa gctgggaacg ccagagctaa tggaggttgg
      301 tcttagtcag aatcagcgat tttcatcacg tctgaaactc tgatcttttt caaatggctg
      361 ttcttgttga tgaacagatt aaactggcga ataaaaggta tactcgccgc cttcaaagca
      421 agaggtttgt ctgtatccct gatttggtgg ttcaggcgag tgttgatctc atcatcgtca
      481 tctgtataca acaggcgaga aaggcggtct tctgatatac gtactttata taattttgac
      541 cataccagaa tgtcgatcat gggaaggagt ctgtaattaa tgattttctt aatcgttcca
      601 taaccaaagc gaatagcttc cgttacatct ggttctatgc cacgcacttt acgccattga
      661 ggaagtgcag cctttaggga ttcagcgatc tcttcatctg tcccgtttgc taggtcgatt
      721 tcgaccatga cagtatccct gaacaatttt ggcaaaactt ccgatacagg gctatcgagg
      781 aattctctgt taacagaata ctcatcgctt ccatcccaaa aaaacagtga gttccgtaat
      841 ccaactatgc tgagttgagc cagtctttcg gttgtcgtca aaaagaagtg cgggggctgg
      901 taaagagtat cctctcttgt gagatagccg agatgctttt ctgtgatgag aaagggcttt
      961 ccagtgaaga tcctgtcgat gtagagcctt acggcttctg cttcaaattc ttcatgatat
     1021 ggtttgaaga acaatgtcct tgccagcaat tcatggtaga ggtgtataag cggaagctct
     1081 tcgaatccgc gatatgtatc tatatccagc cacttcttca cctctctcgt atgttcaact
     1141 gaccagtttt tcataacgat ctccaacagg cattagactt aatgtatgta gcaaacgtaa
     1201 tcgacatgtt aaaggaagac caatgaaatg agttcagatg ccatcaggaa gttgaaccaa
     1261 aaggtttgat attttttgat acagttcgct aacggactat tttcaggggt ttatgccgtt
     1321 acgatttatg gttcctttat agaaccaaag gtaaagtcag aaacagcagt tatctagaaa
     1381 attcaatcaa atagatatct agttacaggg acgccagcca atcatgattg gacggtgtaa
     1441 ggacaacacc aacaaaaaca ggaagttaga agtctcagca ggacaccgac cagacggtga
     1501 ggagacataa aaggatacgc aaaggagccg cggctcctgg tgacatgaaa gcccacagat
     1561 gtgggctttt tcgttgatgg tcagaacgac cagttcacac cagctaccgc gttccacggg
     1621 gattccacac cggcaccatg gctatacccc accccaagat gcccgcttaa cgtactgctg
     1681 aatgaggctt taatacctgc ctggtatatt ccacgtctgc ccgacaaatc attgacgaaa
     1741 ttaccgtcat tattcacctt cacccggtta tcatcgacaa attctttgcg cacagccgcc
     1801 ttcagccacg gctcaatttc cataccgttc cccagacgca tgttgtaact cagcgttgcg
     1861 cccagttcac gatatatact gcgggtatcg actgatttcg attccatgcc attggataaa
     1921 tgatattcgg ggttatcagc ggtgaacccc gttaacgatg catacggcgt caggttccag
     1981 ttaccatcgg taaatcgcat cccggtttca atgtgaccgc ccagcccgtt gctgtggtaa
     2041 ctgccattgg cggctccacc gctgctcatt ttaccggcta cgttactttc aaaacggttc
     2101 agcttcacga caccgtccag atagaaacca ctttcatgtt cccaactggc atagccgccc
     2161 agagaataac tgcccacact gccatgtcct ccgcgatcaa aaccgatatg tgaatgggaa
     2221 taacccataa aagcgcccag cgtcgcaatc ccctcaggaa tatcattagg gctgtcgatc
     2281 cccactgtca ttccggtcag cgtctgctca aacccggccc ccgcatcggt ggtgacatta
     2341 ttacgggtgt tatacgtcgc cccccagaca ttattgttgt gtggactcgc tttcattatg
     2401 ttcaaccgct cgcgaatact gtttagctca gcatcaaata ccaacggtaa tgttgctgcc
     2461 atattgagta cggctgcggt agaaggcgtg atgcgtttct ccggaacggg tgtcggcgtt
     2521 ggctcgggag tcgggtctgg tttcggatcc ggttttgggt ctggttttgg atccggcttc
     2581 ggatttgggt tgggatttgg gttgggatcc gggttgggtt tgacatcatt ggtcaggttc
     2641 cagttgctgt tgccatcgct tttcaggaca tactcatagg tcccaagatc aacgaaaccg
     2701 ccagtattgc ccagcgaaaa cgaagcatcc cctccccctg ttttcaccag cgtcatcgcg
     2761 tcgtcagact gaggactgac gccggtatcc tgaacaaaga ttttaaaatt accagtggcg
     2821 ttgttgttga cgaccagttg atcgccccga gagcctgcaa cgttggtatg caggtagaaa
     2881 ttaccgctac cggaaagttc attggttgtc agcgtattgt atataccgtt agttgtggca
     2941 ggcgctgctg tttgtgctga taaataaaca ttaccgtcgt taagtagcaa actgtttact
     3001 ctatactcgc agttgctggt gcctgcacag gaattattgc tgttaagcca gacactgccc
     3061 cggctggcag ccgtcaggtc ggcaagcgtc gtatcaacgc cattgccgat agttaatgtc
     3121 gcgctatcgg taatccggat cgccccttcg agtttaactg gcgtaacatt atcccgtggc
     3181 gtcattaacg acaggcttcc tgtcgcgcca ctgaccgatg catccgccag cgtacctgcg
     3241 tagacgattg ctgtcccgcc agcaacctgg agatcttctg cccgggccag agcctgaaac
     3301 tcatcttttg accgcccaag ggtatattgc ccaccagagt taacctttgt ggcggagtcc
     3361 tgaccctggt tttgcattgc cccccccttg ccaaccgtgg agtcgcgagc ttcggtacct
     3421 gctaatacca ataaattacc gccattttcc agcaacatat tggtcgctaa attgccggaa
     3481 atggaaaaag tgccgtactg gtgagtaccg ctgatttcaa taccgttagc cgtgctcgtc
     3541 tggagagcgg caccgctgtt ctggacgata tctgtcgctt tgccattatc gttgactgtc
     3601 agcgtaccgc cttcattgat cgttgttttt attgcctctc cgttagctga aactgtttgt
     3661 attccgccgt cgttaattgt tgtctcattc gccacaccct cgacaatttg ttcaccgccg
     3721 gtgagcgtcg tgcctgtcgc agtggctttt gttttgacga tctcccgtcc gcccgtattg
     3781 acctgtgttt tgtcagaaga ggtgtctgac tccacggtta acactccgcc atttgccagc
     3841 aggatattgt tcgccgcacc ctgctcgatg ctgaacgcga cgccatccgc gcgtgttcct
     3901 gtgacccgcg tcgccctggt ggttgcaacc aacgcgccct ggctactctg ctgtatcccc
     3961 gttgcgctgc ctttctcccg cacatcgagt gtgccgccat cattaagcac cgagttttca
     4021 gccagaccgc cctcattaac tatctgtgaa ccgccattaa taatggaacc ttccgctgtc
     4081 ccgtttgcca taatttgttg taggccagag acgatatcgg tattgatcgc cttaccataa
     4141 ttctgaacgg tttgcgtgcc accattgatg tgtgttttct ctgttgaccc accatcaaca
     4201 atttgttcac cactttcgat atttgcttca gtggctaaac catataccgt ttgcttgcca
     4261 cctttgatat ttgctttatc agaagtggca ctggcatata ttgtttgggt gccagcacta
     4321 ttgagtacag tgccaacatc ttttccataa acatccattt tgccgttggc attaataatc
     4381 gtgtcaactg cccgggaacc agtgacgact gttaatgagc cagcgttttc cagcactaca
     4441 tttttagctt ctgaattcct gatgtagaaa gcatcaccat aactgttggt tccttcgata
     4501 agtgttccgg aggtcgtgga agcaattaat gcgccgccgg attgttgctc aacatgctta
     4561 gcctcaccac cgtcctgaac ctccagaacg ccgccattat taagtctggt gctatctgtt
     4621 ttagcctcct tctggacaat cagcttaccg ccagcatcaa tggtagtatt tttcgccgag
     4681 gttttagcca ctaccgtcag ttcgccggta ttttccagca caacataatt agcctcccct
     4741 ccggtaatag tgaagtgaga gagcttgttg tatccttcga tatcagtccc tgcacccgtg
     4801 ttggcaacta aagcactgcc cgtctcctgg ttaaccccat gtgcaatacc gccggtatag
     4861 acaatcagcg agcctccggc gctaatattg ctgccaattg ccgtaccatc tttctcaaca
     4921 acctgccggc tcccggagga tataattgtt gtgtcagctt tcccgccgct tttgatattt
     4981 tgcgttccgc tgttgatatt ggtgcctgtg gctatgccat aattattaat attctgtgtg
     5041 ccaccattaa ttatggtatt tgtcgcgttt cctgcaacat ccataacccc gccattatct
     5101 attcgggtcg catcagcttt agcattggtt aaaactgaca ttgttccttt atctttaata
     5161 atcgtcttgt ttgccgaacc atatgcgttt atgtctaaat gaccaccgtt ttccagcaac
     5221 acattgtctg ccacgtgatt gtggatggag aatgcacctt cactattcgt accgctcacc
     5281 gtcgtaccgt tagtgttagt ttttaaaatt gcaccatcgt gctgggtaac atttgttgcc
     5341 gtaccaccac taacatcaag cacgccaccg gaataaactt caataacatc cgaggagctg
     5401 gtgttatcaa taatttgcgt accaccagaa tagatctgag tattttttgc cgttgactta
     5461 ctattcagag actgagttcc gccttcaatc gtcgtgtcca gcgcacggct ctcatatact
     5521 ctttgctcac cgccattttt gatggtggtt gtttctactg tgctctgttc aacatactgt
     5581 cgaccaccat ttatggttgt gttcgttgcc agacttcctt gtactacgtc ctgagagcca
     5641 gacttattta tcgttgtacc atcagcatgc ccttgaactt ttactatctg gctaccacca
     5701 tcaatgagtg ttccattcgc actccctccc tctacgcgtg aagcaccgcc cttaattgtc
     5761 gttccattgc tgataccccc tttataaacg tcctgattgc cactctcgat tatcgtacct
     5821 gtggaaatac ccccgtcatg aattgactgt ctgcccccgt taatggtggt attattagac
     5881 tgccccacat aattgttatg ccttcctaca tcttgatatc caccagattc aataagactt
     5941 ccattagata ccccgccata aacattctgc tggccatggt tgataatatg agtgttattt
     6001 gttgtacctc gttcatctac tcgttggctg ccatctacag tctcatcgtt taccacacca
     6061 ataacatcag gagtgaaggc agccatcccg ggcggggcat atatcaaggc agatatcaat
     6121 aaggaaagta ctgagcgacg acaataatag ggactggtcc tgttcataaa tttcatcctc
     6181 tgaaaagtga atactgagta gcgtttaagc gaccttagct ttgctgcaac atcagcccac
     6241 aggcaccaga ccaggggatt catcctgaag agacagcgca agtgtattgt gttcaccgct
     6301 catcaaagac atcatgatga aatgatgata ttccgcataa gaatgaggca ttttttaaac
     6361 gcagtgcgct gaagtgtggt tggataaaaa agtcaatcca ttcaggaaat acgggcgtat
     6421 tcttttcttt cgacaatgag gccgttggca aaataaaatg atttacataa tcgtttctga
     6481 tgaatatctt ctgctcacat aaaaatcaca caataacttt gagatcgcag attgttttac
     6541 ttttacagca ttcgtccccc cattgttggg caaatataga ttgggccaga gcacgaaagt
     6601 taataccacg ttcgcacagc tcctccaaca gcacgacaag atgccacata ctgcgcccca
     6661 gtcggttcag tttacagact agcagtgctt tccctgccga tgatgtcctg agcagttttt
     6721 tcagtccgga cctcctgcca cggttttacg ttgaagcggt aaccctgagc acgcagttct
     6781 tcagtcaggc gtggtgcacc gtaacgctgt tattgctggg tgagatcaaa aaactttcag
     6841 gcagctaagg aaagttgaac cagacaatag atgaaatatt tcaaccaatt acagcaccaa
     6901 ttcagtcact gccagcccac caaataaatc aaggggttac atgaaaacgt agcccctttt
     6961 tctttggtag tgacactaaa atggatgtag tgtgaagaat aatcccgttt actcaatcaa
     7021 taatacatat tgtttcaatc tacgttatta tctctttata aaaattgcca tttatttatc
     7081 attgaaaact gcttttagaa ctttatacaa gaggactatt cacaacagga ctattctcaa
     7141 tgggagcatc ctcaggggag ttatcatcaa agtcatcatc cataaataaa atatcatcga
     7201 atggtgccac acccgtgatc atttttattt ttttattacg atcagataag actccattta
     7261 aaccgttttc gcttacaggt tttaatgatt tttcataact ctcatggtta gcaggcgtat
     7321 taaaaataca cggagtatca acatcaaaca atgacattcc ccagttcaca tattgaatat
     7381 catagttact gaagttctgt ccagaaaaga agcatccctt aaaatccaat ccacgtagat
     7441 tatataagcc accctcttct ttttggagag taatgttaat tttggctatc tcccggacat
     7501 catcgccatt tttgtatttg aataccgttt caagatgttc tccagacagc ttgacttctg
     7561 gaaataattt gaaatcaaag cctacattat tatgtgtcaa cgtagatgaa caaaagatgg
     7621 aaaaagcttg cagtgctgaa ttatagctat cgattttatc ttgaggctgc gctcttggta
     7681 aaaacgtata gcaacattca taaaaattaa acagaagctc tgaagatctt ccatttttat
     7741 caaataatat ccccttgaac tccttcacaa acgcatctgt tattttttga atatctatgg
     7801 ggtgttcctc tgactctgac aaagatgaaa cctcatctat tttgttttca tatgaattac
     7861 gtggttccat acagacattt ggtggcattt ctaaaataac accattcata ctacctggct
     7921 taagtaaacc aacatgaaaa tcacttttta ttatattatc gaaaagggtt tgttcatttc
     7981 ttttagacca ttcaactaat tgctcaatct cacctttatt cgctgattta tgaacttcag
     8041 aagccacatt ttccttgatc tccggtttta atttaatgag aagattttcc cagacctgct
     8101 tgcttaaaca tactacgtca ggctcaccat cagtaactaa cacttcatta ttatcaaagt
     8161 gtacgttgaa tacctttaag ttattttcat caacatcata tttaatacgt tttaattgtt
     8221 ccccagctcc cataatgaca aaggcgttgc cttcttgata tatacattca gacattattt
     8281 tttgtaaaat ttcaagagca ccgcgatacg tctctgatgc agcttcctta caaattaatt
     8341 ttacaatact atgagctagt agctccatgt gtttagaaga tttattttca ttataacttc
     8401 cactacccca aactgcgcta gcgttaaatc cgttgctttt attaattaac attttctatt
     8461 cctcagt
//
LOCUS       DAZKIP010000162         8374 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZKIP010000162 DAZKIP010000000
VERSION     DAZKIP010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.164"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(153..229)
                     /locus_tag="ACHOUK_004204"
     tRNA            complement(153..229)
                     /locus_tag="ACHOUK_004204"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(193..195),aa:Asp,seq:gtc)
     gene            complement(362..1093)
                     /gene="dnaQ"
                     /locus_tag="ACHOUK_004205"
     CDS             complement(362..1093)
                     /gene="dnaQ"
                     /locus_tag="ACHOUK_004205"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFP9899540.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRLVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            1158..1625
                     /gene="rnhA"
                     /locus_tag="ACHOUK_004206"
     CDS             1158..1625
                     /gene="rnhA"
                     /locus_tag="ACHOUK_004206"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFP9899541.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1622..2344)
                     /locus_tag="ACHOUK_004207"
     CDS             complement(1622..2344)
                     /locus_tag="ACHOUK_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9899542.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFY
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2378..3133
                     /gene="gloB"
                     /locus_tag="ACHOUK_004208"
     CDS             2378..3133
                     /gene="gloB"
                     /locus_tag="ACHOUK_004208"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFP9899543.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            3205..4563
                     /gene="mltD"
                     /locus_tag="ACHOUK_004209"
     CDS             3205..4563
                     /gene="mltD"
                     /locus_tag="ACHOUK_004209"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFP9899544.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNSMP
                     DS"
     gene            complement(4611..5381)
                     /locus_tag="ACHOUK_004210"
     CDS             complement(4611..5381)
                     /locus_tag="ACHOUK_004210"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9899545.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQLLAVCLADYP
                     DASVLDMGCGAGHASFVAAQNVSTVVAYDLSAQMLDVVAQAAEVRQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDIWL
                     QTVEALCDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVDAHKAA"
     gene            complement(5459..6259)
                     /locus_tag="ACHOUK_004211"
     CDS             complement(5459..6259)
                     /locus_tag="ACHOUK_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFP9899546.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6500..7414
                     /gene="yafC"
                     /locus_tag="ACHOUK_004212"
     CDS             6500..7414
                     /gene="yafC"
                     /locus_tag="ACHOUK_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFP9899547.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPVACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(7411..8214)
                     /gene="dkgB"
                     /locus_tag="ACHOUK_004213"
     CDS             complement(7411..8214)
                     /gene="dkgB"
                     /locus_tag="ACHOUK_004213"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFP9899548.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGLAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKEGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT         2233 a         1998 c         2044 g         2099 t
ORIGIN      
        1 attgaatttt aatgaatgag acaacaagcg agaatcacgc acttacgcca gagtaaaatc
       61 attgaacaga gaaatggtgg aattcaaata caaaaaaaac cgcaaaatta aaaatcttgc
      121 ggttctctga actcattttc atgaggtgaa tttggcggaa cggacgggac tcgaacccgc
      181 gaccccctgc gtgacaggca ggtattctaa ccgactgaac taccgctccg cgttgtgttc
      241 cgttgggaac gaggcgaata ttacggattg cctcaccctt cgtcaacggt ttttctcatc
      301 ttttgaatca tttgctgcaa aaatcgccca agtcgctatt tttagcgcct tttacatgta
      361 tttatgcccg ccagaggcag cttccgccct ttttctgcac cagatcgaga cgggcttcat
      421 gtgctgcaag ctcttcatct gtcgcaaaaa caacgcgtaa cttacttgcc tgacgtacaa
      481 ggcgctggat tgttgcttca ccttgttgct gttgtgtctc tccttccatc gcaaaagcca
      541 tcgacgtttg accaccggtc atcgccagat aaacttccgc aaggatctgg gcatcgagta
      601 atgccccgtg cagcgttcgt ttactgttat ctatttcgta gcgggcacat aacgcatcga
      661 ggctgttgcg tttaccggga aacattttcc tcgccaccgc aaggctatcg gtgaccttac
      721 agaaggtatt agtcttcgga atatcgcgct taagcaacga gaactcgtag tccataaagc
      781 cgatatcgaa cgctgcgtta tggatcacca actccgcgcc gcggatatag tccatgaact
      841 catcggctac ttcggcaaac gtgggcttat cgagcaaaaa ttcatcggca ataccatgta
      901 cgccaaaggc ttccggatcc accagccgat cgggtttgag ataaacatgg aagttattgc
      961 ccgtcaggcg acggttcacc acttcaacgg caccaatctc aatgatcttg tggccttcat
     1021 agtgcgcacc aatctggttc ataccggtgg tttcggtatc gagaacgatc tggcgtgtaa
     1081 ttgcagtgct catagcggtc atttatgtca gacttgtcgt tttacagttc gattcaatta
     1141 caggaagtct accagagatg cttaaacagg tagaaatttt caccgatggt tcgtgtctgg
     1201 gtaatcctgg acctgggggt tacggcgcta ttttacgcta tcgcggacgc gagaaaacct
     1261 ttagcgcagg ctacacccgc accaccaaca accgtatgga gttgatggcc gctattgtcg
     1321 cgctggaggc gttaaaagaa cattgcgaag tcattttgag taccgacagc cagtatgtcc
     1381 gccagggtat cacccagtgg atccataact ggaaaaaacg tggctggaaa accgcagaca
     1441 aaaaaccagt aaaaaatgtc gatctctggc aacgtcttga tgctgcattg gggcagcatc
     1501 aaatcaaatg ggaatgggtt aaaggccatg ccggacaccc ggaaaacgaa cgctgtgatg
     1561 aactggctcg tgccgcggcg atgaatccca cactggaaga tacgggctac caagtcgaag
     1621 tttaagcctg tggtttacga cattgccggg tggctccaac cgcctggcga attcgtggct
     1681 tgtttttact ctgtttcatc ggatttagcg ttaaaggaat agtccgtttc cgggcaacaa
     1741 taagttgtaa gcaaccaagc gcaggaatat gcgcattcaa aagttttcct ccgtgtttgt
     1801 tccacgggag aacgtggaaa cggctggcgt gtagcacttc aaaattcaac aaagagagcc
     1861 agtccagttg ccgcatcaga gtaaacatcc ggctgttata gggcgaggtt ttgcgcaata
     1921 ccggcacaag tttgcgtaat cccattaaac taatgggatt gaagccacta atgaccagcc
     1981 agccatcatc aatcaatacc cgatcggctt cacgcaataa acgatgcgga tcggtgcacc
     2041 acggcaatgt atgtgccagt agacaaacat caacggattt atcggcaaaa ggaagatgaa
     2101 gtgggtccgc ctgcacctgg acaggcattc cttgcgcaga aacattcact tgatgagaaa
     2161 ccgcgcacgc ttcgcaattg atttctgcgc ttaaattgcc aatcttaagc agataaaaac
     2221 catacatttt agtgaaccac gggttgagct ggcgctccag cgccttgcga taaagctctc
     2281 cccagggcaa atcgccccag cgatcaggag ccacgacagt ttgagggact cttgccggtt
     2341 tcatcacaac cttccgtttc acactgagag gtaatctatg aatcttaaca gtattcccgc
     2401 ctttgatgac aattacatct gggttttgaa tgatgaagca ggtcgctgcc tgattgtcga
     2461 tcccggagac gcagagccag tattaaacgc cattgccgcc aataactggc aaccggaggc
     2521 catatttctc acccaccatc atcacgatca cgttggcggc gtaaaagaac tggtggaaaa
     2581 gtttccgcaa attgtggtgt atggtccaca agagacacaa gataagggaa caacacaggt
     2641 agtcaaagat ggcgaaactg ccttcgtttt ggggcatgaa tttagtgtaa ttgccacgcc
     2701 gggtcacact ttaggacata tctgttactt cagtaaacct tatctatttt gcggcgacac
     2761 gctgttttct ggtgggtgtg gtcggttgtt tgaagggaca gcatcacaaa tgtatcaatc
     2821 acttaaaaag ttaagtgcgt tacctgacga tacattggta tgttgtgctc atgaatatac
     2881 gttatcaaat atgaagtttg ctttgagtat tcttccgcac gatttgtcca taaatgatta
     2941 ttatcgtaaa gttaaggagt tacgggcaaa aaatcaaata acactacccg taattctgaa
     3001 aaatgagcgg caaattaatg tttttttaag aacggaagat attgatttaa ttaatgtaat
     3061 taatgaagaa acattattgc aacaacctga agagcgtttt gcatggttaa ggtcaaagaa
     3121 agataggttc tgataaaact ttcttgtcat cggctccgtt cgccgttatg atcggtcgtc
     3181 ttttaagcaa ctattgacgc acacatgaag gcaaaagcga tattactcgc ctctgtcctg
     3241 ctcgtgggtt gccagagtac cggcaacgtt caacagcacg cacagagcct ttctgcagct
     3301 ggtcaagggg aagcagcaaa gtttacaagt caggcacgat ggatggacga tgggacgtct
     3361 atcgcgccag atggtgactt gtgggctttc attggcgacg agctaaagat gggaattccg
     3421 gaaaatgacc ggattcgcga acagaaacag aaatatttac gcaataagag ctatctccac
     3481 gatgtaactt tacgggcaga gccgtatatg tactggatag ccgggcaagt taaaaaacgt
     3541 aacatgccta tggaactggt actactaccc atagtggaga gcgcttttga tcctcacgca
     3601 acgtctggcg ccaatgccgc aggcatctgg cagatcattc cgagcacggg gcgcaattat
     3661 ggtttgaaac agacccgcaa ttatgacgcg cgtcgcgatg ttgttgcttc aacaactgcc
     3721 gcgctgaata tgatgcagcg tctgaacaaa atgtttgatg gcgactggct tctgaccgta
     3781 gcggcttata acagcggcga aggtcgggtc atgaaggcaa ttaaaacgaa caaagcgcgt
     3841 gggaaatcca cggacttctg gtcgttaccg ttgccgcagg aaacgaagca gtacgtgcct
     3901 aaaatgctgg cattgagtga tattctcaaa aacagcaagc gttatggcgt acgtctgcca
     3961 acgaccgatg aaagccgtgc tctggcgcgt gtgcacctga gtagcccggt tgaaatggcg
     4021 aaggttgcag atatggcggg gatttccgtc agcaagctga agacattcaa cgctggcgtg
     4081 aaaggctcca cgctgggcgc aagtggtccg cagtacgtga tggtgccaaa gaagcatgca
     4141 gatcaactgc gtgaatctct ggcttcaggc gaaattgctg ctgtacagtc gacgctggtt
     4201 gccgacaata cgccgcttaa cagccgtgtt tacaccgtac gctctggcga cacgctttca
     4261 agtatcgctt cacgtctcgg cgtaagcacc aaagatttgc agcagtggaa caaactgcgc
     4321 ggctctaagc tgaaaccagg ccaaagtctg acgattggcg caggtagtag cgcacagcgg
     4381 ttggcaaaca acagcgatag cattacgtat cgtgtgcgca aaggcgattc gctttcaagc
     4441 attgctaaac gccacggcgt gaacatcaaa gatgtgatgc gctggaacag cgatactgcg
     4501 aatctgcaac caggcgataa gctgacgttg tttgtgaaaa acaacagcat gccagactcc
     4561 tgacaaacca gataatagaa aggcaccgat tcccccggtg ccttttttat ttatgccgct
     4621 ttatgtgcat ctaccatgat gatatcactg gtgaaagagc catcattctg caaggcaaaa
     4681 tacgttctca cctctgtcga tgcgctctgt tggtaaatgc gaatagcgtc tactaacgct
     4741 tctggcgtac gcattctcgc gacccatgaa gaaaattcca gcggtaactt atctgtaatt
     4801 aaattatcaa ctatcagatt ggcttcattg attaacctca accactcacc gctggcgtag
     4861 tttcgtacgt gagaggtatc gcataatgct tctaccgtct gtaaccagat gtcgcgcact
     4921 gggtggcccg gagacattac gtccatcaca atcagcctac cgccaggttt caatatccgg
     4981 ttcacttcac gcagtgctgc accaacatca tgccagtgat gggcagaata acggctgata
     5041 acaatatcaa atgcgttatc ggcaaatggc agactttcgg catacccctg gcgggtggtg
     5101 atatttttca gttgccggac ttcggcagct tgtgccacga catccagcat ttgggcagat
     5161 aagtcatacg ccaccaccgt gctcacattt tgcgcagcga caaagctggc atgccctgct
     5221 ccgcagccca tatcaagtac acttgcatca ggataatcag ccagacacac cgccaggagc
     5281 tgtaaatctc gtccggatgc atgtacggta ctggtcagat attcgctggc ttgagagcta
     5341 aactgctttt ctacgtggtc atggtgggat tgtgttgtca ttgcactgtc cttttgttgt
     5401 ttgagtataa aagggcagct ccaaacgttg gtctgccctg tggcagacct gacatacctt
     5461 atttatcagg cttgccggga ctgaattcaa cgagtagcgg attgtgatcg gacgcgcgcg
     5521 taaccagtac agaagcttcg ctgacgttca gaccacggta gaaaacaaaa tcgagcgggc
     5581 gaccaaacgc ccgacggcgc tgatcatcgg taaaacgcac ctgacgcagc gacatttccc
     5641 gcgcaaaacg atataacgcg ttcatccttc tacggctcca agcattgaaa tctcccgcca
     5701 taatgaccgg gccgctgtgg tgagctatct gatcaccaat aggaagtaac tgcttactat
     5761 aaacatccac gcccagactg aagttgacgg cgtgtatatt aaccaccatt aacaggcggg
     5821 tgtcaggtaa tggatagacc gtcaccagtg ccgacttcgc cagacgcaaa atgggttctc
     5881 gttcacgtaa cgggcagcaa tacactggat gtgccgccga aagggtcatt acgccagaag
     5941 gatgttgtgg cagcacgaaa gcgggtacct gatcggcggc aagatagtta gcagtcgcaa
     6001 actgtaccaa ctctggcgtt gtttgcgctt cctgcaataa caccagatgt gcgtctttgc
     6061 cgtaattctt taataccgac aaccattcag cgcgttgctg tttgtatatg ttccacacca
     6121 ggatccgaat acgctcttcg gtacttaacg gttctccggg tggtaatgcc tggccgatgc
     6181 tcgcaaaaga ccccggcggt aagatcctct ccgcaggttg tccggcaaca tagcgcatgg
     6241 cataggtgtt ttttcgcact tttgactttg aaacctctat tacgtcaacc cttccgcact
     6301 cgggagggtt ctactgttat agggatttta gctcactctt tcaacgagca attgtatatt
     6361 gttatgtaag caagtgctta caccggtcag aattttgctc attattgaga acattaaatc
     6421 ggatgacata tcacgcatcc tgatggttta atgacaaata aattcaaaat atctcatcta
     6481 ttttgcaaca ggagcaaaaa tgaaagccac gtcggaagaa ctcgccattt ttgtttcggt
     6541 cgtcgaaagc ggcagcttta gccgggcagc ggaacaatta gggcaagcaa actcagcggt
     6601 aagccgggcg gtgaaaaagc tggagatgaa acttggcgtt agcctgctta atcggaccac
     6661 gcggcaactt agcctgacgg aagaaggcga gcgttatttt cgtcgcgtac agtcaatttt
     6721 gcaggagatg gcagcggcag aatcagaaat tatggagacg cgtaatacac cgcgtggact
     6781 gttgcgcatc gatgccgcaa ctccagtggt gctgcacttt ctgatgccgt tgattaagcc
     6841 ttttcgtgaa cgctatccgg aagtcacttt atcgctagtc tcctccgaaa cgattattaa
     6901 tttgatcgaa agaaaagtgg atgtcgcgat acgcgccggt acgttaacgg attccagctt
     6961 acgtgccagg ccgttattta acagttatcg aaaaattatc gcctcccccg attatatttc
     7021 ccgctacggg aagccagaaa cgatcgacga tttaaagcaa catgtttgcc tgggattcac
     7081 tgaacccgct tccctcaata cctggccggt agcctgtagc gatggacaat tacatgaggt
     7141 gaagtacggt ttgtcatcca atagtgggga aacactgaaa cagctttgcc tgagtggcaa
     7201 cgggattgcg tgtttgtccg actatatgat cgacagagaa atcgctcgcg gagaattggt
     7261 ggagttaatg gcagataaag tgttgccagt ggaaatgcca ttcagtgccg tctattacag
     7321 cgaccgtgcg gtaagtacgc gcatccgggc ttttatcgat ttccttagcg agcatgtaaa
     7381 aacagctccc ggaggagctg tcagagaggc ttaatcccat tcaggagcca gaccttccgg
     7441 gctaaccagg cggtcgttgc aatccagtgc ggcgatcgct tttttatctt cggcatcaag
     7501 ctgtaaattt tgtgccttaa gattactctc caggttttca cgtttagtag aagaaggaat
     7561 tactgagtaa ccttccccca tagcccacgc cagaatcact tgtgccggag tcgcattgtg
     7621 tttagctgcg atacgagcaa taacctcatc tttcagggcc ttaccatacg ccagcgtcat
     7681 ataggaagta atatggatgc cgtgctgttt agcccaggca accactttac ggttttgcag
     7741 ataaggagag agttcaatct ggttagtagc gatgttttca gcgccaacag cagcaatcgc
     7801 cttttccatc aatgggatcg tgaagttgga aataccgatc tcacgcgtca acccttcttt
     7861 tttggcttcc agcagcgcct gcataaactc ttcaacagag acttcatcgt ttggtgacgg
     7921 ccagtggatt agcgtcagat caacataatc ggtacgcaat ttttgcaggc tctcttttag
     7981 actcgggatc aatttgtctt tgctgagatt ttcaatccag attttagtgg tgatgtagag
     8041 ttcatgacgt ggcacgccac tttctgcaat cgccagacct actgcggctt cgttatcata
     8101 gatttgtgca gtatcaattg cgcgataacc aagttcaagc gccgttttca cagatgaaat
     8161 aacaacgtcg tctttcagac ggaaagtacc taaaccaaat gcagggatag ccataatatg
     8221 cctcttttaa tgctattttc gttaactgag gtgattatgg gctttagaat gttgcggaaa
     8281 aagggcgtaa aacgcagagg atttttgcga ttttagcaat aatagaaatg tagacgaaaa
     8341 aaaacctcga gcgataactc gaggcttctt aata
//
LOCUS       DAZKIP010000163         8357 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZKIP010000163 DAZKIP010000000
VERSION     DAZKIP010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.165"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..379
                     /locus_tag="ACHOUK_004214"
     CDS             <1..379
                     /locus_tag="ACHOUK_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899549.1"
                     /translation="KENAWHLYKEWIRSDDVSPLFIEIEDNLRTFNTNELTRNDNIFI
                     LFSSVDDGPVMVVSSQRLHDMLNPTKDTNWNSTYIYKSRHEMLPVNLTPETLFGSKSY
                     DKHALFPIFTASWRANRIKNKGI"
     gene            602..1099
                     /gene="ubiC"
                     /locus_tag="ACHOUK_004215"
     CDS             602..1099
                     /gene="ubiC"
                     /locus_tag="ACHOUK_004215"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFP9899550.1"
                     /translation="MSHPALTQLRALRYCKEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1112..1984
                     /gene="ubiA"
                     /locus_tag="ACHOUK_004216"
     CDS             1112..1984
                     /gene="ubiA"
                     /locus_tag="ACHOUK_004216"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFP9899551.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2139..4562)
                     /gene="plsB"
                     /locus_tag="ACHOUK_004217"
     CDS             complement(2139..4562)
                     /gene="plsB"
                     /locus_tag="ACHOUK_004217"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFP9899552.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPVHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4733..5101
                     /gene="dgkA"
                     /locus_tag="ACHOUK_004218"
     CDS             4733..5101
                     /gene="dgkA"
                     /locus_tag="ACHOUK_004218"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFP9899553.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5211..5819
                     /gene="lexA"
                     /locus_tag="ACHOUK_004219"
     CDS             5211..5819
                     /gene="lexA"
                     /locus_tag="ACHOUK_004219"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFP9899554.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5892..7217
                     /gene="dinF"
                     /locus_tag="ACHOUK_004220"
     CDS             5892..7217
                     /gene="dinF"
                     /locus_tag="ACHOUK_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFP9899555.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVRGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTSWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLASLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7333..7542
                     /gene="yjbJ"
                     /locus_tag="ACHOUK_004221"
     CDS             7333..7542
                     /gene="yjbJ"
                     /locus_tag="ACHOUK_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFP9899556.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7584..8099)
                     /gene="zur"
                     /locus_tag="ACHOUK_004222"
     CDS             complement(7584..8099)
                     /gene="zur"
                     /locus_tag="ACHOUK_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFP9899557.1"
                     /translation="MEKTTTQELLAQADKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         1910 a         2025 c         2269 g         2153 t
ORIGIN      
        1 caaagaaaat gcctggcatc tttataaaga gtggattcgt agtgatgatg tttcaccttt
       61 atttatagaa attgaagata atttaagaac ctttaacacg aatgaattaa cacgaaacga
      121 taatatcttt atcttgttct cctctgtcga tgatgggcca gttatggtgg taagctccca
      181 gcgcttacat gatatgttga atcctacaaa agataccaat tggaattcca cgtatatcta
      241 taaatccaga catgagatgt tgcctgttaa tcttactccg gaaacacttt tcggctccaa
      301 atcttatgat aaacatgcgc ttttccccat ttttactgcg agttggcgag ctaatcgtat
      361 aaagaataaa ggtatttaag taaaggaaaa catcaccgct cttggcatca tgggcggtga
      421 tgcccctatg gttgccctcg ccagcacggg catcggcaaa gcgtaaggtt caacagcgtt
      481 ttaccacttc atgcgattgt tgcgtttttg ttgcgcatta gatcacttaa tttgctttac
      541 atctcccgta aacacttttc tgcgatacaa tgcctttacg ttatgtaacg gagagttcgg
      601 catgtcacac cccgcgttaa cgcaactgcg tgcgctgcgc tattgtaaag agatccctgc
      661 cctggagccg caactgctcg actggctgtt gctggaagat tccatgacaa aacgttttga
      721 acagcaggga aaaacggtga gcgtgacgat gatccgcgaa gggtttgtcg agcagaatga
      781 aatccccgaa gaactgccgc tgctgccgaa agagtctcgt tactggttac gtgaaatttt
      841 gttatgtgcc gatggtgaac cgtggcttgc cggtcgtacc gtcgttcctg tgtcaacgtt
      901 aagcgggccg gagctggcgt tacaaaaatt gggtaaaacg ccgttaggac gctatctgtt
      961 cacatcatcg acattaaccc gggactttat tgagataggc cgtgatgccg gactgtgggg
     1021 gcgacgttcc cgcttgcgat taagcggtaa accgctgttg ctaacagaac tgtttttacc
     1081 ggcgtcaccg ttgtactaag aggaaaaaaa tatggagtgg agtctgacgc agaataagct
     1141 gctggcgttt catcgcttaa tgcgtacgga taagccaatt ggcgcgttac tgctgctctg
     1201 gccaacatta tgggcgttgt gggtggcgac accgggcgtt ccccagctct ggatcctggc
     1261 ggtgtttgtc gcgggtgtct ggctgatgcg cgctgccgga tgtgtggtga atgattatgc
     1321 tgaccgcaag tttgatggtc atgttaagcg cacggcgaac cgaccacttc ccagcggcgc
     1381 ggtaacagag aaagaggcgc gcgcgctgtt tgtagtgctg gtactgattt cgtttttact
     1441 ggtgctgacg ctgaatacga tgaccattct gttgtcgatt gccgcgctag cgctggcgtg
     1501 ggtgtacccg tttatgaagc ggtataccca tctaccgcaa gtggtgctgg gcgcggcgtt
     1561 tggctggtcg attccaatgg cttttgctgc tgtgagtgag tcggtgccat tgagttgctg
     1621 gttaatgttc ctcgccaata ttctctgggc ggtggcttac gacacgcagt atgcgatggt
     1681 tgaccgtgat gatgatgtga agattggcat taaatccacc gcaatcctgt tcggccaata
     1741 cgataaattg attattggta ttttgcagat tggcgtactg gcgctgatgg cgattatcgg
     1801 tgagttaaat ggcttaggct ggggatatta ctggtcaatt ctggtggctg gcgcgctgtt
     1861 tgtttatcaa caaaaactga ttgccaaccg cgagcgcgaa gcctgcttta aagcatttat
     1921 gaataataac tatgttggtc tggtactgtt tttagggctg gcaatgagtt actggcattt
     1981 ctgatgatgt aaaaaagccg gatgatcatc cggctttctt ctgggttgcc tgatgcgctg
     2041 cgcttctcag gcctacacaa cagatcgcaa tttattgaat ttgcagatta tggaaggccg
     2101 gataaggcgt ttgcgccgca tccggcaatt ctctctgatt acccttcgcc ctgcgtcgca
     2161 ctctcaatcg tcaaacgcac gtctgatgta atcaactccg ccagcaactg ataaaccttc
     2221 atcgtttctg ccggttcggc atcgccgcta tcgctgatat acccttcatc acgcagcgta
     2281 agcaccagag aactgaacac cgccttgtcg aagaactccg gcgcgttgat gccgtgcagc
     2341 acggagagac gttgcgcgac ggtgcggctc tctttctcca gcgtaccgcg gttgatcgac
     2401 gggttggcgc tcaacagcca gaaggtgatg gcataacgtt gcagcgtttc gcgcgcgcct
     2461 gcggcgagca gctgtagcgt gcgagaatgc accgggttga tatgcaactc atcatcttgc
     2521 agggtaatca gcccctgacg ttgcatctca tttgccagcg catcaataac gtccggcaac
     2581 tcgtcgcgat cccagcgcag gaacagctcc gctttcagca ttgggtaaag cacattgacg
     2641 tgctccatca atacgtcgcg ggagatgtgg cgatgctggg tgacaattgc cgccatcagc
     2701 gaaggtagca ccaacatatg cgcaatgttg ttgcgatagt aggtcatcag caccgcttgc
     2761 tcgcgcggca gaatgatgat gtcgccgata gtgtctttct caacttcaaa cttgttcatt
     2821 tgcagcgcgt gatcgataag ctcgctggcg ctggctgaag gaacggtaga gtccgtggag
     2881 taaggcacgt tgcgcatcag atccaggtag cagttgagtt gctcggttaa ctgctcgcgg
     2941 gtgagtgagc gctgacgtga tgccagtagc gcagtacagc acaggttcat ggcgtttgcc
     3001 gcgcctgcgt tgttaatgcg taccatcaga tcggcagcaa tattattgac cgtcggcgtt
     3061 aaccatgccg gacgcactgc ttcgatggga tcgatagatt cacgccagtc tggtacgtgc
     3121 tggttaaggt aggtcatcaa cggcattggt tcaccgaaat tgacgtaacc ctggccgaga
     3181 ttacgcagct tgcttaaacc gcgcagcatc tgcggcaggc tctctttctc tttcgtcgcg
     3241 ccgcgcagtt ctttggcgta agtacccact tccatgacgt gctcataacc gatatagatc
     3301 ggaatcagcg taatcggacg cgtgccgcca cgcagcatcg cctgaatggt catcgacagc
     3361 gtaccggttt tcggatccag caaacgcccc gtacgggaac gaccgccttc tacgaagtac
     3421 tcgacggaat aaccacggct gaacagttcg ccgagatact cacggaaaac ggtggaatag
     3481 agcttattgc ctttaaaagt acggcgaata aagaacgcac ccagacggcg gaaaatcggc
     3541 ccggcaggcc agaaattcag gttgatcccg gcggcgatat gcggcggcac tagcccctgg
     3601 tggtacagca cgtaagaaag cagcaggtag tccatgtgac tgcggtggca aggcacatat
     3661 accagctcat gaccgtcgtg ggccagctgg cgaacgcgct cggcgttatg gacgttgatg
     3721 ccctggtaaa ggcggttcca ggtaaagccc agaatacggt cagtcaggcg gatcatctcg
     3781 taagagaaat tcgccgcaat ctcttccatc agcgcaatcg cgttctgctg cgctttttca
     3841 tgggagattt ttttgctgcg cgcttcatct tctaccgctt tggcaatggc gcgggaggcg
     3901 agcagcttat taaacagatc ctgacgagca ggcagacgtg gtcctacggc agccagacgt
     3961 tgacgggcaa agtgcatacg cgccacgcgc gccagtttct gcgcgatagt tttatccgtg
     4021 ccgtgttcat ccgccatacg gcgcagcgaa actgacggcg agaaacgcac aaaactgtcg
     4081 cgaccgagcc acagtacagc gaaaaatttc tgtacgccgt taagcatacg cagcggcggg
     4141 ttcacttcgc ctttttcacg ccccggcgcg cgaccaaaca tcaccgacac tggcaccatc
     4201 tgcacatcca gatttgggtt gctacggtgc aaatcgagat agtcgtggaa cagcttaata
     4261 gactcttctt tcggcgtgta ataggtgaat acacgcggcc cgccgtgaat gaacacatag
     4321 cgcggcagta gcgtgccgtc gatttccagc ggctctaacg ggtcaggcaa gtcatgtgcc
     4381 agacactggg cgcgcaacgt cagcaaatct gctttcgagt tgtacggtaa aacgtacata
     4441 attggacgag aggtatccag ccccagttcc ggggcaggat ctgccggaat agacttgctt
     4501 tttaccagga tgcttaatgg taaattcagt aatttgtagt aaattcgtgg ccagccggac
     4561 ataaacgatg taaagcctct ggttaataat gcaaatgcgc ggcaaggata gcagaaagtc
     4621 atgggaaatt ctgtggtatc cgctcatgtt tcgcgcggcg ttacgcaaac ccgaatcatc
     4681 ggatttaacg gtacactgat attgacgctc ataatgtaaa aaggttcttt caatggccaa
     4741 taataccact ggattcaccc gaataatcaa agctgctggc tattcctgga aaggtttacg
     4801 cgctgcatgg atcaacgaag cggcattccg tcaggaaggc gtagcggtat tgttggcggt
     4861 ggtcatcgcc tgctggctgg atgtggacgc gattacccgc gtgctgctta tcagctccgt
     4921 gatgctggtg atgattgtgg aaatcctcaa tagcgccatc gaagcagtgg ttgaccgaat
     4981 tggctctgaa taccatgagc tttccggacg cgctaaagat atgggatccg ccgcggtgct
     5041 gattgcgatt atcgtcgccg tgattacctg gtgcatcctg ttatggtcgc attttggata
     5101 acccttccag aattcgataa atctctggtt tattgtgcag tttatggttc caaaatcgcc
     5161 ttttgctgta tatactcaca gcataactgt atatacaccc agggggcgga atgaaagcgt
     5221 taacggccag gcaacaagag gtgtttgatc tcatccgtga tcacatcagc cagacaggta
     5281 tgccgccgac gcgtgcggaa atcgcgcagc gtttggggtt ccgttcccca aacgcggctg
     5341 aagaacatct gaaggcgctg gcacgcaaag gcgtaattga aattgtttcc ggcgcatcac
     5401 gcgggattcg tctgttgcag gaagaggaag aagggttgcc actggtaggt cgtgtggctg
     5461 ccggtgaacc gcttctggcg caacagcata ttgaaggtca ttatcaggtc gatccttcct
     5521 tgttcaagcc gaatgctgat ttcctgctgc gcgtcagcgg gatgtcgatg aaagatatcg
     5581 gcattatgga tggcgacttg ctggcagtgc ataaaactca ggatgtacgt aacggtcagg
     5641 tcgttgtcgc acgtattgat gacgaagtta ccgttaagcg cctgaaaaaa cagggcaata
     5701 aagtcgaact gttgccagaa aatagcgagt ttaaaccaat tgtcgttgac cttcgtcagc
     5761 agagcttcac cattgaaggg ctggcggttg gcgttattcg caacggcgac tggctgtaac
     5821 atatctctga gaccgcgatg ccgcttggcg tcgcggtttg tttttcatct ctcttcatca
     5881 ggcttgtctg catggcattc ctcacttcat ctgataaagc actttggcat ctcgccttac
     5941 ccatgatttt ctccaatatc actgttccgt tgctgggact ggtcgatacg gcggttattg
     6001 gtcatcttga tagtccggtt tatttgggcg gcgtggcggt tggtgcaacg gcgaccagct
     6061 ttctctttat gctgttgctg tttttacgca tgagcaccac cgggctgact gcgcaggctt
     6121 atggtgccaa aaatcctcag gcattagccc gtgcgctggt gcaacctttg ctgttggcgt
     6181 tgggggctgg ggcgttaatt gcgctgctgc gtacgccgat tatcgatctg gcgctgcata
     6241 ttgttggcgg tagtgaagca gtcctggaac aggcgcggcg ctttcttgaa atccgctggt
     6301 taagcgcacc ggcgtcgctg gcgaatctgg tattacttgg ttggttactc ggcgtgcaat
     6361 atgcccgtgc gccagtaatt ttgttagtgg tcggcaatat cctcaacatt gtgctggatg
     6421 tctggctggt gatgggactg catatgaacg tgcggggcgc ggcgctggcg acggttattg
     6481 cggaatatgc aacattgctg attggtctgc taatggtgcg taaaatcctc aaactacgcg
     6541 gaatctccgg cgaaatgctg aaaacttcct ggcgaggaaa cttccgtcgc ttgctggcac
     6601 ttaaccgcga tatcatgctg cgctcgctgt tgttgcaact ctgtttcggc gcgatcaccg
     6661 tacttggcgc gcgactgggg agtgacatta tcgctgttaa cgcggttctg atgacgctac
     6721 tcacctttac cgcctatgcg ctggacggtt ttgcctacgc ggttgaagca cattccggtc
     6781 aggcatacgg tgcgcgcgac ggtagccagt tgctggatgt ctggcgggca gcatgccgcc
     6841 agtcggggat cgtagcgtta ctgttttcgg tggtttattt gctggcaggg gaacacatca
     6901 ttgcgttact ggcgtcgtta acccagattc agcagctggc cgaccgctat cttatctggc
     6961 aggtgatttt gccgttggtc ggcgtctggt gttatctgct ggacggcatg tttataggcg
     7021 caacgcgtgc tgctgaaatg cgtaacagta tggcggtggc cgccgcaggt tttgcgctga
     7081 cgctccttac gctgccgtgg ctgggtaatc atggtttgtg gttggcgtta accgtctttc
     7141 tggcgttgcg cgggctttct ctggcagcta tctggcggcg tcactggcgc aacggtacct
     7201 ggtttgccgc aacgtgacgg ttaaaaattc tgaataaata atcctaagcc aaattgctga
     7261 ctacacttaa tctcacgttc agaagaaaag tgaacgtact ctcattcaca acctaacgat
     7321 gaggtcttga ttatgaataa agatgaagcc ggcggtaact ggaaacagtt taaaggtaaa
     7381 gtgaaagagc aatggggcaa actgaccgat gatgatatga cgatcattga aggtaaacgt
     7441 gatcaactgg tcggtaaaat ccaggaacgt tatggttatc agaaagatca ggcagaaaaa
     7501 gaggtcgtgg attgggaaac ccgcaatgaa tatcgctggt aattaatccc tcctgcccga
     7561 cgtgtacaag gatgtacgcc ctcttaacgc ggtttctttt tcacctgcac agagtgatca
     7621 tgctggcact gttcaggatg acgacacgct tccacttcca cgcatgccgc acagagccca
     7681 tgtgcttcaa tcacattatg ccgcagggca aaccccattt ttgccgccag cgtatgcatg
     7741 atgtcttcca cgccttcagc acattcttct ttcaccgcgc cgcagcgatc gcaaataaac
     7801 atagctgacg tatgggtggg ctggtcgaac agatggcaga gcacataact gttggtggat
     7861 tccaccttat gcacaaaacc ttgctcaagc agaaaatcca gcgcgcgata aaccgttggc
     7921 ggcttggctt gcggttcagc ttcgcgcagc aaatcaagca gatcataagc gctgatagcg
     7981 ccatcctgca gactcatcag gcgcaacact tccaggcgct gtggggtcag gcgcacatta
     8041 cgctgcgcgc agattttgtc agcctgcgct aataactcct gcgttgtggt cttttccatc
     8101 gggcacctca aagtgggggt taagaatgtc cttactttac catgttccag gaaaaacacc
     8161 gagaaccctt attgttgccg taatgttgct tttctgtttt gtaggtaaga tgttgtgttg
     8221 cctggcagta ccattatcaa cacgataata attaataatt catttttaaa taaattgctt
     8281 caagttaact cgctatttat ttaaccgtgg ctaatcgtat acccgaattt cagaaaatta
     8341 tgagcgattt ttgatag
//
LOCUS       DAZKIP010000164         8252 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZKIP010000164 DAZKIP010000000
VERSION     DAZKIP010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.166"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            189..595
                     /gene="ipbA"
                     /locus_tag="ACHOUK_004223"
                     /gene_synonym="fimX"
                     /pseudo
     CDS             189..595
                     /gene="ipbA"
                     /locus_tag="ACHOUK_004223"
                     /gene_synonym="fimX"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="IpbA is also known as HbiF and FimX; frameshifted;
                     incomplete; partial in the middle of a contig; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase IpbA"
     gene            1021..1815
                     /gene="hyxR"
                     /locus_tag="ACHOUK_004224"
                     /pseudo
     CDS             1021..1815
                     /gene="hyxR"
                     /locus_tag="ACHOUK_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304859.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator HyxR"
     gene            1969..2730
                     /gene="hyxA"
                     /locus_tag="ACHOUK_004225"
     CDS             1969..2730
                     /gene="hyxA"
                     /locus_tag="ACHOUK_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein HyxA"
                     /protein_id="HFP9899558.1"
                     /translation="MRFVLFCPSGIVPAQFAALSTGSVGNVTCIGTEEELRNKLRHRP
                     QSVVISAGRPAECAEMWFRFYRDHSFVVVLCVAPFFLPPDVSISGVLKNLRLLKPGMS
                     VEHVISIANSSGGLSGLKHAENLPVMDSYSVFMKEVNNRTKTIVMSERFPEKQKKVLS
                     LLLAGHSWEYSAQFLKTGIRQIWLAEQSLKKRWGIPDSMSLREALLLSSNKFHGDGNA
                     LEKTNAMTLRENGNTNRYSVVVNAGTQVLSLHKYK"
     gene            2772..3854
                     /locus_tag="ACHOUK_004226"
                     /pseudo
     CDS             2772..3854
                     /locus_tag="ACHOUK_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798070.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
     gene            3844..4032
                     /locus_tag="ACHOUK_004227"
     CDS             3844..4032
                     /locus_tag="ACHOUK_004227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFP9899559.1"
                     /translation="MLWLRLGDESVNASIDGAAFRVGAGVQADITKNMGAYASLDYTK
                     GDDIENPLQGVVGINVTW"
     gene            4214..4882
                     /gene="ykgH"
                     /locus_tag="ACHOUK_004228"
                     /pseudo
     CDS             4214..4882
                     /gene="ykgH"
                     /locus_tag="ACHOUK_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
     gene            complement(5125..5820)
                     /gene="ykgG"
                     /locus_tag="ACHOUK_004229"
     CDS             complement(5125..5820)
                     /gene="ykgG"
                     /locus_tag="ACHOUK_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFP9899560.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAKNISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAERLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(5813..7240)
                     /gene="ykgF"
                     /locus_tag="ACHOUK_004230"
     CDS             complement(5813..7240)
                     /gene="ykgF"
                     /locus_tag="ACHOUK_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFP9899561.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGLWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDNVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(7251..7970)
                     /gene="ykgE"
                     /locus_tag="ACHOUK_004231"
     CDS             complement(7251..7970)
                     /gene="ykgE"
                     /locus_tag="ACHOUK_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFP9899562.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         2274 a         1929 c         1822 g         2227 t
ORIGIN      
        1 tctattgcgt agaactacta ttcaaaaaat cttattaatg agataatgag attacatcga
       61 taacgttctg attgcaggca tacttatctg ggtgtgcctc tcttttttac aactgtacac
      121 ggtggcgtat gacgagaaaa tatctcacac aggatgaagt ctacaggctg atggatgcag
      181 cccagagcat gtcctttcct gaaagaaatc gctgtctaat tatgatggct ttcattcacg
      241 gttttagggc cagtgaactt cttgatttac gtttatccga tattgatgca tcaggaaaac
      301 aacttaatat tcggcggatt aaaaatggtt tctcgacaac acatcctctt cttcctgatg
      361 aatataattt gatcaagttg tggcttaagc agaggaagtt aattgaaaac ggagttgaag
      421 gagactggct ttttctctcc cggaaacgcc gccctatcag cagacaacat ttttttctat
      481 cattcgtgag gctggaagac gtgcaggatt agctgtaaaa gcacatcctc atatgttacg
      541 tcatgcctgt ggttttgctc tggctgacaa tggtgtcgat acccgactac ttcaggatta
      601 tctgggtcat cgaaatatcc agcatactgt cagatacaca gccagtaatg ctgctcgatt
      661 taaaggggtg tggaaaaaaa agcctcgtta aaacagtacc ttctgattca aaatgtcaga
      721 ttcctacgtt ttcctcaatt cttccataaa aaaacagccc attcaattca ataaaacaca
      781 taacatgttg atatttctcg ctaaagtaaa ataaccccat gatttgaaat gattaaccgc
      841 caaggtcata ccagtgatta actttcactc atgttgttaa atgcaaccaa attaaaaaca
      901 ataaaaatca aacaaattag atttatattc caagaaaatt aaatattcag gcaattaaac
      961 aaaacctgac attttgaatc aaaatgtcag gttttgtagc tatggcgtgg acgagtctga
     1021 atgaagaagc ctctggttat tatcagtgcc tgtcagttta ccaggctggc actggagtct
     1081 ctcatcccag cggacaggta tatcgtgcgg gtgtacagca acgtcactac agaggttgaa
     1141 ttcgttttaa agacctcctg tggctatctg ttggctgata tcccttagct tccttcgggg
     1201 gagttagttc ttctggcttg tcttgtccgc ttgcggagtg atgccgggca atggcaggtc
     1261 tgcctgaccg gtgactccga ctggttcgat aatgctcctg ctgcaagtct gtataacggc
     1321 ttcccacgca tcggcaccat gctgacagcg agtcgttatc agaatcaaat tatccgctgg
     1381 ttactacgtc ctttgcccgg cgatatgagc gatttctggc tcctgactgc acgggagttg
     1441 agcgtgctta agattctgat gcagggcatg agtttcagtg agattgcccg ttatgaacaa
     1501 cgcagttcaa agaccctgca tgcgattgcc acacgggcat taatgaaact tggactggga
     1561 actctgagcg atttcaggct gctctatacg ggatgcggtg acagcagagt gaaacggaat
     1621 atacggctac atgcaagata tgtcggacaa cattccgctc atcgactcac tcagtatgtg
     1681 cagaacatgg tatccggaat tctttatcca gagaaagttg ccgttggtga atcaataaat
     1741 tctgacgcca tttccggaaa agtacttccc ggacaaagaa aaaatatttc taaaaaaaga
     1801 ccaggattgt tatgacacaa cagcgttgtt actacccccc actgcggcgc gtacggactc
     1861 gctacagtgt aggggccaaa atatttctac tgctctgggc tgcaggattg ggaggcacgc
     1921 tgtacagcct gtacagcgtg ctggcgtact gacatctgtg agggaaggat gcgtttcgtt
     1981 cttttctgtc cctcaggaat tgtaccagcg cagttcgcag ctctatcaac cggttctgtc
     2041 ggcaatgtta cctgcatcgg aacagaagag gagttacgca ataaattgcg gcacaggcca
     2101 cagagtgtcg tgatatcggc agggcgacct gccgaatgcg ctgaaatgtg gttccgattt
     2161 tatcgggatc actcattcgt ggtggtgttg tgcgtcgcgc cattttttct gccaccggat
     2221 gtgagtattt ctggcgttct caaaaacctg aggttactga aaccaggaat gtcagtagag
     2281 catgtcatca gtattgcaaa ttcctctggt gggttatcag ggctgaaaca tgcagaaaat
     2341 ttgccagtta tggacagtta ctctgtcttt atgaaagagg tgaataaccg cacaaagaca
     2401 atcgtcatgt cagaacgctt cccggaaaag caaaagaagg tgttgtcgtt gcttcttgcc
     2461 ggacacagct gggaatacag tgcgcagttt ctcaaaacgg ggataagaca gatatggctt
     2521 gccgagcagt cgctaaaaaa gcgttgggga ataccggaca gcatgtcatt gcgggaagcc
     2581 ctgctgctga gcagcaataa atttcacggt gatggaaatg cattggaaaa aacgaatgcc
     2641 atgacattgc gtgaaaacgg aaataccaac aggtattccg tggtggtaaa tgcagggacg
     2701 caggttttat ctctgcataa atataaataa gtgggaaatt gaaattagtt accagaatgg
     2761 agaatttctt catgaaaaac agtaaggcat tttaccgcag cgcattagcg acagctattg
     2821 ttatggctct ttctgcacca gcattcgctg ctgataacgc ggtatcaact gattcggtta
     2881 cgctgaataa agataagacg actctggatc aagatgttgt tattagcaat acagcagaca
     2941 aacagattac agccgtaaca attaatgcgg cagatgaaga ccttaatgtt gcttttgctg
     3001 gtcatgatat tactgctgaa tcaacagcag acaaaaaatt cgttgaaggt gtaaaagtta
     3061 gtggtaacaa aaatgttgtg attaatgcta cagactccac catcacagct caaggtgaag
     3121 gcacctatgt ccggactgca atggtcattg attcaactgg cgatgttgtt gttaatggcg
     3181 gtaatttcgt tgcaaaaaat gaaaaaggta gtgcgacagg gatatctctg gaaggggcca
     3241 agggaaataa tgtcacgctt aatggcacaa ccataaatgc tcaaggtaat aagagttcca
     3301 gcaacgcctc cacggcaatt tttgctcaaa agggtagctt attgaatagt tttgaaggtg
     3361 atgcaaccga caacattacc cttgctggct caaatattat taatggtcgg attgaaacaa
     3421 tagttactgc cgggaataag acgggaattc atacagtcaa cctgaatatt aaggatggct
     3481 cagtaattgg ggcggctaat aataaacaaa caatttatgc ctctgcttcg gcacaaggca
     3541 caggttcagc aacgcaaaat ttaaatttgt ctgttgctga ttcaaccatc tactctgatg
     3601 tcctggccct ttctgaaagc gagaattcag ccgctaccac aacaaatgta aacatgaacg
     3661 ttgcccgctc ttactgggaa ggtaatgctt atactttcaa tagcggcgat aaagcgggta
     3721 gtaatctgga tataaatctt tccgatagtt cagtctggaa aggcaaagtt tcaggggcag
     3781 gaaatgccag tgtatctctg caaaacgagt ctgtctggaa tgttacggga tcctcaactg
     3841 ttgatgctct ggctccgtct gggtgatgag tctgtcaatg ccagcattga cggtgcagca
     3901 ttccgcgtgg gtgcaggtgt acaggctgat atcaccaaaa acatgggagc atatgcaagc
     3961 cttgactaca ccaaaggtga cgacattgag aatccgctac agggtgtagt tggtatcaat
     4021 gtgacctggt aagaataaag cagtcggttc ccctgtcgac tgttataccc cctgcaggca
     4081 gcagtatgct gcctgcaggc tataaatatc cagtattcat gatgctggct ttttgctgat
     4141 atttaacccg aacattcctg atatagacta ttaattcatt tatctgcggc agattttatt
     4201 ttctggatat attatgagtg aacaaatcaa acaggatatc gatctgattg agattttatt
     4261 ttatctgaag aaaaagattc ttgttatcct ttttattata gctatctgta tggcaatggt
     4321 gcttttattt ctgtatatca ataaagataa tataaaagtg agttacagcc taaaaataaa
     4381 tcagacaaca cccggcatac ttgttaactg tgatagcaat aataactttg cctgtcagac
     4441 aacaatgacg gaagatgtta ttcagcgaat tactacattt tttcacacca gcccagatgt
     4501 caagaacaga gaaataaagc tggaatggtc aggagataag agagatttac caactgctga
     4561 agcggaaata tctcgcgtgc aggcgtctat tatcaaatgg tatgcgtcag aataccataa
     4621 tggcaggtaa gttctcgatg agatacaaac gccttcagca attaacagtg agctttatac
     4681 aaaaatgata tacctgacca ggaactggtc attgtatccg aacggtgatg gctgtgtaac
     4741 tataagctca ccagaaataa aaaataaata ccctgctgcc atttgcctgg ctctgggatt
     4801 ttttctaagc attgtaattt ctgtaatgtt ttgtcttgtc aaaaaaatgg tagatgaata
     4861 ccaacaaaac tctgggcagt aataaacagc attctgatag agagtgcctg tataaagctg
     4921 aagtgacaat gaccgggaag ggaacccgtt ttcaggaatg gatgcctgtc tttttcactg
     4981 taaatatcct ggcattagta atgccaggat atttacagaa gaggagcata accatgacgg
     5041 aacggggtta agtcaggttg tccttaacgc tattttcaac ctcgtatgcc ttcttcaggt
     5101 ttatgtccag acttcatacc tctctcagca atcctcaata atcagataca ccgctttcac
     5161 cggaccatga accccgacga ctttgataag ctcaatgtcc gccgttgagc tgggaccact
     5221 gatgatgtta atacatgacg gcatgcgttc accggcctgc gctttctgat gcaatctttc
     5281 tgcgagttgc gctacacgcg gcaggatagt gcttttacgc aggataaaaa gagatgattc
     5341 cgggagcagg ctcaatgaac gcccacgctc ggcggcggaa aaaagaacca cgcctcccga
     5401 ttcggttaaa ccatattcag catacacgac acccacttta gcctgctctg cctgcgagat
     5461 attcttggca cctttcgccg gatcccaaac aacggcattg cattcctgct gcaaacgttc
     5521 gctaatccct aattcctcca gcctcgtgtc accgctaatc acgaccgact gatctcccag
     5581 ctctttacac agacgtattg cagcttctgc cgccttcgcc tcgctggtca gctcacagcg
     5641 cgtcaacata acatcgctgg caaactgaat aaacgcgtca cagcgctgct gttggttaag
     5701 ttgggtaagc cgctcgttag catagttatt aagcggcgca tcttctgctt gcggttcaag
     5761 tcgcagcggg cgacccagtg cctgagcaac gttattcaaa aattcgcctc gattatccat
     5821 tctttttctc ctgcgcctga tgtttcttaa accaactacg gaaactctct ccgtcagctt
     5881 caggaagatc gcgtgcttcc atccagtcgc taatcgcgcc aaatttgagt ggtgttttgc
     5941 cgccattgat aaaccagctt gccgcatgag caccggccat catcccgact ttccacaatc
     6001 ctggatgact attggcataa gcgaacattt ttatcgcccg ttgctctgct tttgcggtga
     6061 tccctttttc agccatcacc cgacgatgac gcaaaatcag ttttgacagc ggaatacgca
     6121 ccggacacac gttgtcacaa gctgtgcata aagagcaggc gtagggtaaa tctttaaaat
     6181 ctttatagcc gccaagtagc ggagaaatca ccgcaccaat tggccctgga taaatagagc
     6241 catatccatg accgccaata tggcgatatg ccggacaagt attcatacaa gccccgcagc
     6301 gaatacagcg cagcacatcc cgaaattcag aggccagcac ctcagaacgc ccgttatcga
     6361 caataaccag atgaaactct tcaggaccat caacgtgccc cgcttcgcga ggtcctgtca
     6421 gccaggtgtt gtatcccgtc aaacgtgcac caacggcact gcgcgccagc atggtgatca
     6481 atacatctac ctcggcaaac gtgggggcaa tacgctccat tcccatcact gcaatatgcg
     6541 ttttaggcag cgtggtacac attcgcgcat taccttcatt ggtcaccagg cataccgaac
     6601 ctgtctctgc caccgcgaaa ttacagcccg taatacctat ttcagcactg aggaaatctt
     6661 cgcggatttt ttgccggatg aataaggtca tcgcttcagg cgtttccggc ccctcatagc
     6721 ccagacgttc gtgtagcact cgacggatct gatggcgatc tttatgaatt gccgggacca
     6781 caacatgaga gggcggatct tgatccagct ggagaatata ttcacccaaa tcggtttcaa
     6841 tcacctgaat gccagcatcc tgcaacacat gattgacacc aatctcttcg gtcaccatcg
     6901 atttagattt caccaccttc cgggcatttt tgcgttgggc aacctgtaaa atgtagcggg
     6961 tagcgtcttc tttggttttt gcaaaataga cgtgaccgcc gttttgcgtc actttttctg
     7021 agagctggta cagataagcg tcgagattac tcagaacatg atcacgtatc tgggcggccc
     7081 gatcgcgcca ctcctcccag agccccaatt catcgaccat tttttgccga tttgccccaa
     7141 tacgctgctg cgcgtttgcc accgctttgc gcatgatcgg atcttcaatt tgctgacgga
     7201 tgcgtgtctt aaaatctgta ttactggttt tgatcgacat ctttatatcc tcagcggctc
     7261 atcaacactt cagcaatatg catcactttg actttctgcc cttcccgttg taatcgccca
     7321 ctgatgttca gcaggcaact cacgtcagca ccaattaaat actcagggcg gacttccatc
     7381 aggtgcgcaa ccttttcttt caccatctcg ccggatattt cggccatttt gaccgagaac
     7441 gtgccgccaa atccgcagca ggtatcctgt tcagcaaagg tcaacagctc cagtccacgc
     7501 acatttttca gcagcgtaag tggctcgtcc ttcaccccca gcttacgggc caggctacaa
     7561 gatgggtgat acaccgctct cccttgcaaa ctggcaccta catcgactac ccctaattta
     7621 ttaacaataa aagaggtgag atcctgcata cgcgcggcaa ccttttcggc tcgtgatgcc
     7681 cattcaggtt catccgccag atacgtcggg taacttttta cggcataggt gcaagagcca
     7741 gccggtgaaa taatgggatc gtcgttatcc tccagtgcgg cgatcagatt tttcatccct
     7801 ggaatcgctt ctttgatata accactattg atcgcaggct gaccgcagca tccctgtttc
     7861 tccgggaaat ttacgcgaca gccgagtttt tccagtagca gcacggagtc tcgtgccatt
     7921 cttgatttca gggcgtcacc aatacaggtg acaaagaaat tgacattcac aactaatact
     7981 ccattacttc atgcccattt attcccgatg tttcccccca cccggggatc aatcatcaat
     8041 aaatatggcg ttctttttta attggctagc aacataccac cccttatttg tatgacaaat
     8101 gatagtcttt catccaaact tcaaatgcaa taactaattt tatttctttc ggcatcgctc
     8161 acattttatg gggaaatcga cgatcatttt tggcataaga aatccattta aaaatggcta
     8221 catcccttat acctattcag agctacttat tc
//
LOCUS       DAZKIP010000165         8212 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZKIP010000165 DAZKIP010000000
VERSION     DAZKIP010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.167"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(131..1021)
                     /gene="malG"
                     /locus_tag="ACHOUK_004232"
     CDS             complement(131..1021)
                     /gene="malG"
                     /locus_tag="ACHOUK_004232"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFP9899563.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(1036..2580)
                     /gene="malF"
                     /locus_tag="ACHOUK_004233"
     CDS             complement(1036..2580)
                     /gene="malF"
                     /locus_tag="ACHOUK_004233"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFP9899564.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGREQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(2734..3924)
                     /gene="malE"
                     /locus_tag="ACHOUK_004234"
     CDS             complement(2734..3924)
                     /gene="malE"
                     /locus_tag="ACHOUK_004234"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFP9899565.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            4289..5404
                     /gene="malK"
                     /locus_tag="ACHOUK_004235"
     CDS             4289..5404
                     /gene="malK"
                     /locus_tag="ACHOUK_004235"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFP9899566.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            5476..6816
                     /gene="lamB"
                     /locus_tag="ACHOUK_004236"
     CDS             5476..6816
                     /gene="lamB"
                     /locus_tag="ACHOUK_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFP9899567.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEVNPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            6967..7887
                     /gene="malM"
                     /locus_tag="ACHOUK_004237"
     CDS             6967..7887
                     /gene="malM"
                     /locus_tag="ACHOUK_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFP9899568.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            8116..>8212
                     /locus_tag="ACHOUK_004238"
     CDS             8116..>8212
                     /locus_tag="ACHOUK_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899569.1"
                     /translation="MLGHISKFDGNNSLIKHGVVQGNNIVDFDLLR"
BASE COUNT         2010 a         2146 c         2132 g         1924 t
ORIGIN      
        1 attaaccggc gaacgattca gattgcagac gaaagaaaaa aaggcgctcc gtggagcgcc
       61 gaataacagt cacaagttgg gataacgtaa gttgagggtg cagcggcata acattggcag
      121 aacaacatct ttaacctttc acaccacctg ccgtcaggcc gttgaccagc cagcgttgag
      181 ccagcaagaa gacgatggtg atcggtaatg cagacatcac ggcagcggcg gcaaagtcac
      241 cccacaggta gttttgcgga ttgaggtatt gctgcatccc cacggccagg gtgtagctgt
      301 ttacgtcacg cagtaacagc gacgcgaccg gaacttcagt aatggcagcg ataaacgaca
      361 ggatgaatac caccgccaga atcggtactg acagcggcaa caggacaagg cggaaggcct
      421 gccacggtgt cgcaccatcc agcgcagccg cttcttccag cgaactgtcg atggtttcga
      481 aatagccttt gatggtccag acgtgcagcg caatcccacc cagatacgcg aaaattacgc
      541 cgccgtgagt attcaggcca atgaatggaa tgtactcacc cagacgatca aacaacgcat
      601 acaacgcgac cagtgaaagt actgccggga acatctggaa aatcagcatt cctttcagca
      661 gcgtcgcttt gcctggaaag cgcatacggg cgaaagcgta ggcgcaggtg gtggagagcg
      721 ccacaatgcc aatcgcggaa atcccggcga cctttaccga gttccacagc cacagcagca
      781 ccgggaatgg cggtggcgta atgcgaccat cagcctgttc cacgctaaaa cctaatgcca
      841 gtttccagtg atcccaggag atttgctccg ggatcaggct gccggtcgca aagttcccct
      901 ggcgcagtga gatagcgacg accatcagca gcgggaacat aatcgccgcg ataaaaagta
      961 gcagtagcag gtgagtaata aacaaacgtg ctttttgcga tttcggttgg accattgcca
     1021 tttttgttat ctcccttaat caaacttcat tcgcgtggct ttcaggttca ctatcgccag
     1081 cgcacccacc agcaggaaga tcagcgtggc aattgctgct gccagaccga agtcctgacc
     1141 cccgccgcct tcaaaagcga tgcggtaggt gtagttaaca agcaggtcgg tataaccggc
     1201 tggcgtggtc gtgccaagac gatccgggcc gccgttggtt aacagttgaa tcagcacgaa
     1261 gttgttaaag ttaaaggcga agctggcgat catcagcggc gtcagcggtt taatcagcag
     1321 cggcagcgta atcttaaaga agttctggaa cggacctgcg ccatccattg ctgaggcttc
     1381 atacaaatcg tccggaatcg ctttcagcaa gcccatgcag aggatcatca tgtacggata
     1441 gcccagccag gtattgacga taatcagcat cgttcgggcg gtggtcggat cgctgaacca
     1501 ggcgggcttc acgccaaaca gcgcgctcaa catcatgttg atctcaccga agctctggtt
     1561 aaacaaccct ttgaaaatca agattgaaat gaacgatggc accgcgtagg gcagaatcag
     1621 caggacgcga tagaccgctt tgccgcgcaa cgcttcccac tgcaccagac acgccagaac
     1681 catgccgact gccaccgtta aaaagacagt gatcagcgag aacaccacgg tccagacgaa
     1741 aatggcgagg aacggtttct gaatgccttc gtcggtgaag acgcgagtaa agtttttcca
     1801 gccggtggtc acggtgtaac cggggcttag cttttcatca ccccagttgc cgtcggcggt
     1861 aatggactgg taaaagccaa tttggttatt cggacgatat ttcacgccgc tctgattatt
     1921 cgtcaacgtg ccgtcaccgt cgagtgtgta gagcggctgc gtgccagaaa actggcgcag
     1981 ggagctcatc atcactttgt tgccatccgg cagaatggcg gtaatgtcac tcagcgcctg
     2041 acgattctgg gtaatcacgc gtagatttgc gcgttcgcct tcgggctggg cggtcgtttc
     2101 tttcagttgc agtttttgct ctctgccaaa tttaaaagcg tcggagaggt aatttttgcc
     2161 ggtttcgccg tcgctgagcg ccagttgcca ctcatcgccc gccgggtaaa gaccaaagtt
     2221 ataggttttg cctgcttgcc aggagcgatc taacaacact tcctgtgcac gttcaaaagt
     2281 cagctggtta gtgctgctgt agttggtgaa ggcaatggcg atggtgcaga ccagagggaa
     2341 gaggacgaat aatcccattc cagccattcc cgggtaaaca tagcgccagg cgtaggcttt
     2401 acgattggcg aaaatataca gccccgctga actcaatatc agcgtggtaa tggcgaacag
     2461 gtattcccct tgtgcgtaca ttaaaacaac aaggtaaccc accagcaggc cgagcagacc
     2521 taacactgac catttcagcg cgtcgctttg ccaccaatgt ttctttttaa tgacatccat
     2581 ggggttcttc ctcattccag gacggataag gctttcatgc cttatccgac aacaactgcc
     2641 tgatgcgacg ctgacgcgtc ttatcaggcc tacatacgtt tcggttttgt aggccggaca
     2701 aggcgttcac gccgcatccg gcatttcaca gcattacttg gtgatacgag tctgcgcgtc
     2761 tttcagggct tcatcgacag tctgacgacc gctggcggcg ttgatcaccg cagtacgcac
     2821 ggcataccag aaagcggaca tctgcgggat gttcggcatg atttcacctt tctgggcgtt
     2881 ttccatggtg gctgcaatac gtggatcttt cgccaactct tcctcgtaag acttcagcgc
     2941 tacggcaccc agcggtttgt ctttattaac cgcttccaga ccttcgtcag tcagcagata
     3001 gttttcgagg aactctttcg ccagctcttt gttcggactg gcggcgttaa taccggcgct
     3061 cagcacgcca acgaacggtt tagatggttg acccttgaag gtcggcagta ccgttacacc
     3121 ataattcact ttgctggtgt cgatgttgga ccatgcccac gggccgttga tagtcatcgc
     3181 tgtttcgcct ttattaaagg cagcttctgc gatggagtaa tcggtgtctg cattcatgtg
     3241 tttgttttta atcaggtcaa ccaggaaggt cagacccgct ttcgcgccag cgttatccac
     3301 gcccacgtct ttaatgtcgt acttgccgtt ttcatacttg aacgcataac ccccgtcagc
     3361 agcaatcagc ggccaggtga agtacggttc ttgcaggttg aacatcagcg cgctcttacc
     3421 tttcgctttc agttctttat ccagcgccgg gatctcttcc caggtttttg gcgggttcgg
     3481 tagcagatct ttgttataaa tcagcgataa cgcttcaaca gcgatcgggt aagcaatcag
     3541 cttgccgttg taacgtacgg catcccaggt aaacggatac agcttgtcct ggaacgcttt
     3601 gtccggggtg atttcagcca acaggccaga ttgagcgtag ccaccaaagc ggtcgtgcgc
     3661 ccagaagata atgtcagggc catcgccagt tgccgcaacc tgagggaatt tctcttccag
     3721 tttatccgga tgctcaacgg tgactttaat tccggtatct ttctcgaatt tcttaccgac
     3781 ttcagcgaga ccgttatagc ctttatcgcc gttaatccag attaccagtt taccttcttc
     3841 gattttggcg agagccgagg cggaaaacat catcgtcgtt aatgcggata atgcgaggat
     3901 gcgtgcacct gtttttattt tcataatcta tggtccttgt tggtgaagtg ctcgtgaaaa
     3961 cacctaaacg gactctagtt tctttatacg gcaacctctt tccatcctcc ttgcccctac
     4021 gccccaccgt tgctttgtgt gatctctgtt acagaattgg cagtaatgtg gagatgcgca
     4081 cataaaatcg ccacgatttt tgcaagcaac atcacgaaat tccttacatg acctcggttt
     4141 agttcacaga agccgcgttc tcatcctccc gcctcctccc ccataaaaaa gctagggggt
     4201 ggaggattta agccatctcc tgatgacgca tagtcagccc atcatgaatg ttgctgtcga
     4261 tgacaggttg ttacaaaggg agaagggcat ggcgagcgta cagctgcaaa atgtaacgaa
     4321 agcctggggc gaggtcgtgg tatcgaaaga tatcaatctc gatatccatg aaggtgaatt
     4381 cgtggtgttt gtcggaccgt ctggctgcgg taaatcgact ttactgcgca tgattgccgg
     4441 gcttgagacg atcaccagcg gcgacctgtt catcggtgag aaacggatga atgacactcc
     4501 gccagcagaa cgtggcgttg gtatggtgtt tcagtcctac gcgctctatc cccacctgtc
     4561 agtagcagaa aacatgtcat ttggcctgaa actggcaggc gcaaaaaaag aggtgattaa
     4621 ccaacgcgtc aaccaggtgg cggaagtgct acaactggcg catttgctgg atcgcaaacc
     4681 gaaagcgctc tccggtggtc agcgtcagcg tgtggcgatt ggccgtacgc tggtggccga
     4741 gccaagcgta tttttgctcg atgaaccgct ctccaacctc gatgctgcac tgcgtgtgca
     4801 aatgcgtatc gaaatctccc gtctgcataa acgcctgggc cgcacaatga tttacgtcac
     4861 ccacgatcag gtcgaagcga tgacgctggc cgacaaaatc gtggtgctgg acgccggtcg
     4921 cgtggcgcag gttgggaaac cgctggagct gtaccactat ccggcagacc gttttgtcgc
     4981 cggatttatc ggttcgccaa agatgaactt cctgccggta aaagtgaccg ccaccgcaat
     5041 cgatcaagtg caggtggagc tgccgatgcc aaatcgtcag caagtctggc tgccagttga
     5101 aagccgtgat gtccaggttg gagccaatat gtcgctgggt attcgcccgg aacatctact
     5161 gccgagtgat atcgctgacg tcatccttga gggtgaagtt caggtcgtcg agcaactcgg
     5221 caacgaaacc caaatccata tccagatccc ttccattcgt caaaacctgg tgtaccgcca
     5281 gaacgacgtg gtgttggtag aagaaggtgc cacattcgct atcggcctgc cgccagagcg
     5341 ttgccatctg ttccgtgagg atggcactgc atgtcgtcga ctgcataagg agccgggcgt
     5401 ttaagcaccc cacaaaacac acaaagcctg tcacaggtga tgtgaaaaaa gaaaagcaat
     5461 gactcaggag atagaatgat gattactctg cgcaaacttc ctctggcggt tgccgtcgca
     5521 gcgggcgtaa tgtctgctca ggcaatggct gttgatttcc acggctatgc acgttccggt
     5581 attggctgga caggtagcgg cggtgaacaa cagtgtttcc agactaccgg tgctcaaagt
     5641 aaataccgtc ttggcaacga atgtgaaact tatgctgaat taaaattggg tcaggaagtg
     5701 tggaaagagg gcgataagag cttctatttc gacactaacg tggcctattc cgtcgcgcaa
     5761 cagaatgact gggaagctac cgacccggcc ttccgtgaag caaacgtgca gggtaaaaac
     5821 ctgatcgaat ggctgccagg ctccaccatc tgggcaggta agcgcttcta ccaacgtcat
     5881 gacgttcata tgatcgactt ctactactgg gatatttctg gtcctggtgc cggtctggaa
     5941 aacatcgatg ttggcttcgg taaactctct ctggcagcaa cccgctcctc tgaagcaggt
     6001 ggttcttcct ctttcgccag caacaatatt tatgactata ccaacgaaac cgcgaacgac
     6061 gttttcgatg tgcgtttagc gcagatggaa gtcaacccgg gcggcacatt agaactgggt
     6121 gtcgactacg gtcgtgccaa cctgcgtgat aactatcgtc tggttgatgg cgcatcgaaa
     6181 gacggctggt tattcactgc tgaacatact cagagtgtcc tgaagggctt taacaagttt
     6241 gttgttcagt acgctactga ctcgatgacc tcgcagggta aaggtctgtc gcagggttct
     6301 ggcgttgcat ttgataacga aaaatttgcc tacaatatca acaacaacgg tcacatgctg
     6361 cgtatcctcg accacggtgc gatctccatg ggcgacaact gggacatgat gtacgtgggt
     6421 atgtaccagg atatcaactg ggataacgac aacggcacca agtggtggac cgtcggtatt
     6481 cgcccgatgt acaagtggac gccaatcatg agcaccgtga tggaaatcgg ctacgacaac
     6541 gtcgaatccc agcgcaccgg cgacaagaac aatcagtaca aaattaccct cgcacaacaa
     6601 tggcaggctg gcgacagcat ctggtcacgc ccggctattc gtgtcttcgc aacctacgcc
     6661 aagtgggatg agaaatgggg ttacgactac aacggcgata gcaaggttaa cccgaactac
     6721 ggcaaagccg ttcctgctga tttcaacggc ggcagcttcg gtcgtggcga cagcgacgag
     6781 tggaccttcg gtgcccagat ggaaatctgg tggtaatagc aaaacctggg ccggataagg
     6841 cgtttacgcc gcatccggca taaaacaggt tgtcattatc tgaaaggggc gaaagcccct
     6901 ctgattatcg ggtttagcgc gctattgcct ggctaccgct gagctccaga ttttgaggtg
     6961 aaaacaatga aaatgaataa aagtctcatc gccctctgtt tatcagcagg gttactggca
     7021 agcgcgcctg gaatcagcct tgccgatgtt aactacgtac cgcaaaacac cagcgacgcg
     7081 ccagccattc catctgccgc gctgcaacaa ctcacctgga caccggtcga tcaatctaaa
     7141 acccagacca cccaactggc gaccggcggc caacaactga acgttcccgg catcagtggt
     7201 ccggttgctg cgtacagcgt cccggcaaac attggcgaac tgaccctgac gctgaccagc
     7261 gaagtgaaca aacaaaccag cgtttttgcg ccgaacgtgc tgattcttga tcagaacatg
     7321 accccatcag ccttcttccc cagcagttat ttcacctacc aggaaccagg cgtgatgagt
     7381 gcagatcggc tggaaggcgt tatgcgcctg acaccggcgt tggggcagca aaaactttat
     7441 gttctggtct ttaccacgga aaaagatctc cagcagacga cccaactgct cgacccggct
     7501 aaagcctatg ccaaaggcgt tggtaactcg atcccggata ttcccgatcc ggttgctcgt
     7561 cataccaccg atggcttact gaaactgaaa gtgaaaacga actccagctc cagcgtgttg
     7621 gtcgggcctt tatttggttc ttccgctcca gctccggtta cggtaggcaa cacggcggca
     7681 ccagctgtgg ctgcacccgc tccggtgccg gtgaagaaaa gcgagccgat gctcaacgac
     7741 acggaaagtt attttaatac cgcgatcaaa aacgctgtcg cgaaaggtga tgttgataag
     7801 gcgttaaaac tgcttgatga agctgaacgc ctgggatcga catctgcccg ttccaccttt
     7861 atcagcagtg taaaaggcaa ggggtaatta cgccccacag tgctgatttt gcaacaactg
     7921 gtgcgtctcc tggcgcacct ttttttatgc ttcattccag ggatatgagc gattttttat
     7981 agtaactcac ttcttcttca ctaagaatat ccattatctc aatgcattat cagagattct
     8041 tttcctttcg ccggtagtgt ctggacgctc aggctacttt ttccagttat ttcatttctg
     8101 taatgcagag gttttatgtt aggtcatatc tcaaagtttg acggcaataa ctctttgata
     8161 aaacatggtg tggtgcaagg aaataatata gtagattttg atttactacg ta
//
LOCUS       DAZKIP010000166         8195 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZKIP010000166 DAZKIP010000000
VERSION     DAZKIP010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8195
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.168"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..113
                     /locus_tag="ACHOUK_004239"
     CDS             <1..113
                     /locus_tag="ACHOUK_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFP9899570.1"
                     /translation="TRAAHPLSRESIAVSMIVGRDNCAFDSDRGNFVLKN"
     gene            144..278
                     /locus_tag="ACHOUK_004240"
     CDS             144..278
                     /locus_tag="ACHOUK_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HFP9899571.1"
                     /translation="MKLILGTACKTRHLVCKNVLRLAGELSIVRVLNDFQPLLILLVN
                     "
     gene            complement(275..517)
                     /locus_tag="ACHOUK_004241"
     CDS             complement(275..517)
                     /locus_tag="ACHOUK_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFP9899572.1"
                     /translation="MPHRYPAAKINKMPKGSVPALQQEMLRRVSKRYDDVEVIIKSTS
                     NDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVR"
     gene            649..1932
                     /gene="ydfJ"
                     /locus_tag="ACHOUK_004242"
                     /pseudo
     CDS             649..1932
                     /gene="ydfJ"
                     /locus_tag="ACHOUK_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
     gene            2021..3481
                     /gene="ydfI"
                     /locus_tag="ACHOUK_004243"
     CDS             2021..3481
                     /gene="ydfI"
                     /locus_tag="ACHOUK_004243"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFP9899573.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLHQQDNLYTVAEMSADAWTARVVGVVKKALHVQI
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYALMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GGDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(3517..3720)
                     /gene="ydfZ"
                     /locus_tag="ACHOUK_004244"
     CDS             complement(3517..3720)
                     /gene="ydfZ"
                     /locus_tag="ACHOUK_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFP9899574.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(3897..4583)
                     /gene="rspR"
                     /locus_tag="ACHOUK_004245"
                     /gene_synonym="ydfH"
     CDS             complement(3897..4583)
                     /gene="rspR"
                     /locus_tag="ACHOUK_004245"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFP9899575.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(4672..5418)
                     /gene="ydfG"
                     /locus_tag="ACHOUK_004246"
     CDS             complement(4672..5418)
                     /gene="ydfG"
                     /locus_tag="ACHOUK_004246"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFP9899576.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASIEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            5555..7600
                     /gene="dcp"
                     /locus_tag="ACHOUK_004247"
     CDS             5555..7600
                     /gene="dcp"
                     /locus_tag="ACHOUK_004247"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFP9899577.1"
                     /translation="MTTINPFLVQSTLPYLAPHFDQIANHHYRPAFDEGIQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQATLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(7645..8163)
                     /gene="ydeJ"
                     /locus_tag="ACHOUK_004248"
     CDS             complement(7645..8163)
                     /gene="ydeJ"
                     /locus_tag="ACHOUK_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFP9899578.1"
                     /translation="MNINRNKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
BASE COUNT         2064 a         1899 c         2069 g         2163 t
ORIGIN      
        1 aaacaagggc ggctcatcct ttgagcaggg aatcaatcgc agtttcaatg attgtaggaa
       61 gagataattg tgcttttgac tctgacagag gtaacttcgt tttaaaaaat taagatagca
      121 atggcgggtt aacttttcgt aaaatgaaat taattctcgg tacagcatgt aaaacgcggc
      181 acctcgtatg caagaacgtg ctgcggttgg ctggtgaact ttcgatagtg cgagtattga
      241 atgatttcca gccgttactg attttactcg ttaattagcg aacaaaccac tcgtcagcag
      301 actcccaggt atctttcagc gtctcctgaa caaaagtttt tgcagaatct ttatcggcgg
      361 tgcgagtaac tgaaaggcca tcgttgctgg tggatttgat gatcacttct acatcgtcat
      421 aacgtttact gacacgtcgc agcatttctt gttgcagggc aggaacagaa ccttttggca
      481 ttttgttgat tttggctgcg gggtatctgt ggggcatata tgggacataa aaaggcctca
      541 aataaacaga aaaatcgaga ttagggattt ttagaaaaaa tacaaccatc tgaaaaaact
      601 tagaaaacac ccaaaaaccc acagaatagt aaaaaacatc tatgaattat ggatttccag
      661 ttatattcgc tcggcgcagc gttagtgttt catgaaatat tttttcctga atcatcaacg
      721 gcaatggcgt taattctggc aatgggaacc tacggtgcag gttatgtggc gcgtattgtc
      781 ggagcattta ttttcggcaa aatgggcgac agaatagggc gtaaaaaagt gctctttatt
      841 accatcacca tgatggggat ctgtaccacc ttaattggag tgttaccgac ctatgcacag
      901 attggtgttt ttgcccccat cttgctggtg acgctgcgta ttattcaggg attgggtgca
      961 ggtgcggaaa tttccggtgc cggtacgatg ctggcggaat atgcgccaaa aggtaagcgc
     1021 ggaattatct cctcatttgt ggctatggga actaactgcg gaaccttgag cgcaacggca
     1081 atctgggcct ttatgttctt cattctcagt aaagaggaac tgctggcgtg gggatggcgt
     1141 ataccgttcc tggcgagcgt tgtcgtgatg gtctttgcta tctggttgcg tatgaatctg
     1201 aaagaaagcc cggtctttga gaaggttaac gacagcaacc aaccgacagc aaaacctgca
     1261 cctgctggta gcatattcca gagcaaatcc ttctggctgg caacagggct gcgttttggt
     1321 caggcaggta actccgggtt aattcagact ttccttgcag gctatttagt acagacgtta
     1381 ttgtttaaca aagcaattcc aacagatgca ttgatgatca gttcgattcc cggctttatg
     1441 accattccgt tccttggttg gttatccgat aaaattggtc gccggatccc gtatattatt
     1501 atgaatacct ccgcgattgt gctggcatgg ccaatgcttt ctatcatcgt agataaaagc
     1561 tatgccccga gcaccattat ggttgcactg attgtgattc ataactgtgc ggtgctggga
     1621 ttatttgctc tggaaaacat taccatggca gaaatgttcg gctgtaaaaa ccgctttacc
     1681 cggatggcta tttctaaaga aattggtggt cttatcgctt ccggttttgg tcctatcctg
     1741 gcgggtattt tctgcaccat gacggaatcc tggtatccga tcgccattat gatcatggca
     1801 tattcagtga ttggtttaat ctctgcgctg aaaatgccag aagtgaaaga ccgtgattta
     1861 agtgcgctgg aagacgctgc ggaagatcaa ccgcgtgttg taagagctgc gcaaccttcc
     1921 agaagtctgt aaatcctcaa tcccttctct taccggagag gggattttta ttcatataac
     1981 aaaacatgta gcttgccata tttaaattta aggaattgtt atgggaaata atttgttatc
     2041 agcaaaagcg acactccctg tttatgatcg taataacctg gccccaagaa ttgttcattt
     2101 aggctttggt gcatttcacc gtgcgcatca gggcgtgtat gccgatattc ttgctacaga
     2161 acatttcagt gactggggat attatgaggt caacttaatc ggcggcgaac agcaaattgc
     2221 cgatttacat cagcaagata atctttatac cgttgcggaa atgtcggccg atgcgtggac
     2281 ggctcgcgtc gttggcgtcg ttaaaaaagc cttgcacgta cagatagatg gcttagaaac
     2341 cgtgttggct gcgatgtgtg aaccgcaaat cgcgattgtc tctctgacaa tcaccgaaaa
     2401 agggtatttc cactctccgg cgaccggaca gttaatgctc gatcacccga tggtcgctgc
     2461 cgacgtgcaa aatccccacc agccgaaaac agcaacaggg gtgattgttg aggcgctggc
     2521 tcgccgtaaa gcggcaggac ttcccgcatt taccgtcatg tcatgtgaca acatgccaga
     2581 aaacggtcat gttatgcgtg acgttgtcac ttcctacgca caagccgttg atgtaaaact
     2641 agcacaatgg atcgaagata acgtgacttt cccatcaaca atggtggacc gtattgtgcc
     2701 cgcagtgaca gaggatacgc tggcgaaaat cgaacagctt accggtgtgc gcgatcctgc
     2761 tggcgttgcc tgtgaacctt tccgccagtg ggtaatcgaa gataactttg ttgccggacg
     2821 tccggaatgg gaaaaagcgg gagccgaact ggttagtgat gtgctgcctt atgaagagat
     2881 gaagttgcgc atgctcaacg gcagtcattc attcctggcg tatctgggtt atcttgccgg
     2941 atatcagcac attaatgact gtatggaaga tgaacattat cgtcatgcgg cgtatgcctt
     3001 gatgttgcag gaacaagcgc cgacgctgaa agtgcagggc gttgatttgc aagattacgc
     3061 taaccgatta attgcacgct atagcaaccc ggcgttacgt catcgaacct ggcagattgc
     3121 gatggatggc agccagaaat tgccacagcg gatgttggat tctgttcgct ggcatctggc
     3181 gcatgacagc aagttcgatc tgctggcgct gggcgtcgcg ggttggatgc gttatgtcgg
     3241 tggtgttgat gaacagggaa atccgataga aatcagtgac ccactgttac ctgttattca
     3301 gaaggctgta caaagtagtg ccgaagggaa agcgcgcgtc cagtcattgc tggcgattaa
     3361 ggcgattttt ggtggtgatt tgccagacaa tagcttgttt actgcaaaag tgacggaagc
     3421 gtacttgtct ttattagcgc atggtgcgaa agcgaccgtg gcgaaatatt ccgtgaagta
     3481 aaaaaagcaa tgccgccatc ggcggcattc acacgcttag ttcatgccga gacgaatcag
     3541 gtccagtggt gccagtttct cttcacaacc ttcaacaact acggtttttc cgcggcggat
     3601 ttgctcagcg gtcagacctt cagtatcaat cgacaggatc ttgccagtgt gaccggtgcc
     3661 gctaaccata acacggctgc cagtggtgat tgcgttacgg ttacgatcgt aggtcgtcat
     3721 aggtgttttc tcctttctga tttacagtcg aagcccacgt aacgggcgca atataaatac
     3781 tcctgtcgag tgaaatattt tttgtttttg atcaagctca catctttttt gatgcatgca
     3841 tgagccataa ggcgggggaa aagactctca ccccggatgg gatgagagga aatgaattac
     3901 tcttcgctaa accagtcgct attttcctgg cggatctggc gcacggattc gctgatttcc
     3961 tgcaaatgtt gcgtcattgc acgttctacc gcatcgccat cacgtttttg cagggcagag
     4021 aatatatcaa gatgctggcg taacagcatt tctggtggag aaacatggtc gaaactcata
     4081 tagcgcacgc gatcaacggt cgctttcagg ttctcaatgg tatcccacgc cagttgacag
     4141 tcggcaatct gcgtcaggag ttgatggaag ttgtcatcaa gttcaaaaaa atcatccagt
     4201 tgcttgcgct caatggcaat gcgttgctgg tgaagatttt gttccagttg gtagcactgg
     4261 ctttcggtaa tcatactcgc cgcccgacgc gccaccgcgc actcaatggc ctgacggata
     4321 aaactgccgt tgcgcacctg ggccatggaa attttgttga cgtagctgcc acgctgcgga
     4381 cgaatttgaa tcaggccgtt ttccgccagt ttaataaagg cttcacgaac cggctggcgt
     4441 gacacattga aacgaacaga aacttctttt tccgacaacg gtgtgcctgg tgcaatcagg
     4501 caatggacaa tgtcgcgacg aagaatacga taaatctgct gattgacagg ctgtgtggga
     4561 ttaagttgcg tttcgacggt cattcgttct tgcttgtgag tgagttaact gcatgagtct
     4621 accacttttt tgtgacaagc aataacccgg cagttacacc gggtataaaa attactgacg
     4681 gtggacattc agcccggcat agctttgggt aaccggcatc atttccaggg tattgatatt
     4741 gacgtgagca ggcagcgttg acacccacca gacggcttcg ctgacatctt ctggcgttaa
     4801 tgcaacggta ttttgatagg ttttttccgc tttaccgtca tcgcctttaa agcggacatt
     4861 ggaaaactcg gtgccaccca ccagacccgg ttcgatgtcg gtgacgcgca ccgccgtacc
     4921 atgcaggtcc gtacgcagat tcaggctaaa ctgacgaaca aacgctttcg tcgcaccgta
     4981 aacgttacca ccggcatacg gccagctacc tgccgttgag ccaatgttaa taatatgacc
     5041 atgattacgt tcaaccatac ccggtaagac ggcgcgcgtc atatatacca ggcctttgtt
     5101 gttggtatca atcatcgttt cccagtcttc aatgctggct ttatgcgcag gctccatgcc
     5161 caacgccaga ccggcattat ttaccaggat atcaatattg caccactcgg caggaagcga
     5221 tgccagcatc tcttcaatag cggcgcggtt gcgaacgtcc agttgggcga tgtacagatt
     5281 atctcccagt tcgtctttta actcctgcaa ccgttcctgg cgacggccag tggcgataac
     5341 tttatgccct tgttgaataa aacgacgagt aatgcattca ccaaaacctg ccgttgctcc
     5401 agttactaaa acgatcatct cactgttcct caacgctttt gtgtgtcaat aacatagcac
     5461 gtgatgctta agagcgggta atactggttt gcaagattgc actccgttat cagtaagcct
     5521 acgctatgga acacctcgcg ttcaggagaa gaaaatgaca acaattaatc ctttccttgt
     5581 gcaaagcaca ctgccgtatc tggctcctca ttttgatcaa attgccaatc atcactatcg
     5641 cccggcattc gatgagggaa tacagcaaaa gcgggcagaa attgctgcca tcgcgcttaa
     5701 cccgcaaaca cctgatttca acaatactat tctggcactg gaacaaagcg gagaattact
     5761 tacccgcgtt accagcgtct tttttgcgat gactgcggcg cataccaatg atgaattaca
     5821 gcgtcttgac gagcagtttt ccgctgaact ggcggaactg gctaatgata tctatctgaa
     5881 cggtgaatta ttcgcgcggg tagatgctgt ctggcaacgc cgtgaatctc tggggcttga
     5941 tagtgaatcc atccgcctgg tggaggtgat tcatcaacgt tttgtcctag ccggagccaa
     6001 acttgcacaa gctgataaag caaaattaaa agtactgaat acagaagctg cgacactgac
     6061 cagtcagttt aaccagcggt tactggcagc aaataaatcc ggcggtctgg ttgtgaacga
     6121 tatcgcgcag ctggcaggaa tgagtgagca agagattgcg ctggcggcag aggcggctcg
     6181 cgagaaaggt ctggataaca aatggctgat tccgctgctg aataccaccc aacaaccggc
     6241 gcttgccgaa atgcgcgatc gtgcgacgcg tgaaaaactg tttattgcgg gctggacgcg
     6301 agcggaaaaa aatgatgcca atgatacccg cgctatcatt caacgtctgg tggagatccg
     6361 tgcacaacag gctacattac tcggttttcc tcattatgcc gcatggaaaa tcgccgatca
     6421 gatggcaaaa acacctgaag cagcactcaa ctttatgcgg gaaattgttc cagcggcgcg
     6481 tcaacgtgcg agcgatgaat tagcctccat acaggcggtt attgataagc agcagggcgg
     6541 gtttagcgcg cagccgtggg actgggcatt ttatgccgaa caggtccggc gagagaaatt
     6601 tgatcttgat gaggcgcagc tcaagccata ttttgaatta aacacggtgt tgaatgaagg
     6661 tgtattctgg accgcgaatc agctcttcgg tattaagttt gtcgaacgtt ttgatattcc
     6721 tgtctaccat cctgacgttc gggtgtggga aatttttgat cataatggcg tggggctggc
     6781 gttattttac ggtgatttct tcgcccgtga ttcaaaaagc ggcggtgcat ggatgggcaa
     6841 ttttgttgag caatcaacgc ttaatgaaac gcatccggta atttataacg tctgcaatta
     6901 tcagaaaccc gctgccggtg agcctgcgtt gttactctgg gatgatgtca taaccttatt
     6961 ccatgaattt ggtcatacgc tgcacggcct ttttgcccgc cagcgttatg ccacgctctc
     7021 cggcaccaac acgccgcgtg attttgtcga atttccgtcg caaatcaacg aacactgggc
     7081 aacgcatccg caggtattcg ctcgctacgc ccggcattat cagagcgggg cagcaatgcc
     7141 tgacgaactg caacagaaaa tgcgtaatgc cagcctgttc aacaaagggt atgagatgag
     7201 cgaactgctt agcgccgcac ttctcgatat gcgctggcat tgcctggaag aaaacgaagc
     7261 aatgcaggat gtcgatgatt ttgaattgcg ggcgctggtg gcagaaaata tggatcttcc
     7321 tgctataccg ccacgctatc gcagcagtta ttttgcccat atttttggtg gcggatatgc
     7381 cgcaggttat tacgcttatc tgtggacgca aatgttggcc gatgatggtt accagtggtt
     7441 tgttgagcag ggcggattaa cgcgtgaaaa tgggctgcgt tttcgcgagg cgatcctttc
     7501 cagaggtaac agcgaagatc tggaacgcct gtatcgacaa tggcgcggta aggctcctca
     7561 gattatgccg atgctgcaac atcgtggctt gaacatataa acaccaccaa tggctgtcct
     7621 ggagatatcg ccaggccagc ctggttatag cagtaactgc agcagctggg cgagggcaaa
     7681 cctcaccgct aaagctaata ccgtttcgca gtcgccagca aaatgcataa ccgcagtgta
     7741 gttctggcct ttaatatgcc acgcgaacca gacggtaccc gctggcgtac catcttcacc
     7801 gccctccggt ccgccgtagc cggtaatggc aatactgaca tcagcatccg cacgctctat
     7861 ggcaccggtt gccatttctg ctgccacttt ctcactcacc gcagaatatc gttcaagaga
     7921 ttgctggctt acgctgagga ttttcatctt tgcctgatcg gtgaaagtaa caaagcctgc
     7981 accgtaaaat ttgggtgtat cttcagctgc acacagggcg ctagccaact taccgccggt
     8041 gcatgattcg gcggtagtta agcgtaattg atccgcgata agtctttgac tcaacgcgga
     8101 tgtcaggttt tcaatagtgt cggtatctgc aagttgtaca attttatttc tattaatgtt
     8161 cactgttttt tgtccacgct tttatatgta aaggt
//
LOCUS       DAZKIP010000167         8079 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZKIP010000167 DAZKIP010000000
VERSION     DAZKIP010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8079
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.169"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            75..617
                     /locus_tag="ACHOUK_004249"
     CDS             75..617
                     /locus_tag="ACHOUK_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9899579.1"
                     /translation="MKHSIIAVAVLSSVFMSAGIFAAEVDTGTLTIKGNIAESPCTFV
                     AGGDSVSINMPTVPTTVFDGKAKYSTYDGAVGVTSSMLKITCPKTIDGVKLSLITNEK
                     ILGNDKAISSSNDTVGYYLYLGDSSDVLDISAPFNIESYKTADGQYAIPFKAKYLKLT
                     DSGVASGTVNSSLVMRVAQD"
     gene            705..1379
                     /locus_tag="ACHOUK_004250"
     CDS             705..1379
                     /locus_tag="ACHOUK_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP9899580.1"
                     /translation="MKGLLSLLISSMIFPVHAGMVIFGTRIIYPAEQKEVMVQLMNQG
                     NRSSLMQAWIDDGDTSLPPEKIQVPFMLTPPVVKVAANSGQQIKIKIMPNKLPSNKES
                     IFYLNVLDIPPNSPEDEGKNALKFAMQNRIKLFYRPAGIAPVNKTTFKKLRVSRSGNN
                     VVIKNDSPNWVTISDVKANNVKVNYETIMIAPQESQNVSVKNNNANGWQLTIIDDHGN
                     YISDKI"
     gene            1395..3875
                     /locus_tag="ACHOUK_004251"
     CDS             1395..3875
                     /locus_tag="ACHOUK_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFP9899581.1"
                     /translation="MLRMTPLASAIVALLLGIEAHAAEETFDTHFMMGGMKGEQVTNL
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPHETCLTREIVKRLGINSDNFAREN
                     QCLTFEQLVQGGSYSWDIGIFRLDLAVPQAWVEELENGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTDNNPGEWKSNALYLEHGF
                     SQILGTLRIGDMYTSADIFDSVRFTGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFSISDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFLSQTSTRFGLAAWRYSSRDYRT
                     FNDYVWANNKDNYRRDKNDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYDENHAEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGNRDQFNYGINLSHQHQGNETTAGANL
                     TWTAPAATVNGSYSQSSTYRQVGASVSGGLVAWSGGVNLANRLSETFAVMHAPGIKDA
                     YVNGQKYRTTNCNGVVVYDGLTPYRENHLMMDVSQSDSETELRGNRKMTAPYRGAVVL
                     VDFDTDQRKPWFIKALRSDGQPLTFGYEVNDMHGHNIGVVGQGSQIFIRTNEIPPAVN
                     VAIDKQQGLSCTITFGKEIDESKNYICR"
     gene            3886..4920
                     /locus_tag="ACHOUK_004252"
     CDS             3886..4920
                     /locus_tag="ACHOUK_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9899582.1"
                     /translation="MMFRNRILLIFILWANFTWAGCRTTASLNITDGINVGEILANET
                     SFSKSVVFTGISCDTSTDKIVYKNIQSDWVEVGPFGNGEKLKVKIESLGKTSDTIGKS
                     SNAQAVLPYVVKIARGTPDFTGERKSTWFISDTVIANIGGESSSSIDFWLGICKALKF
                     NWCVNYLTSKLAGDTFTLGLNISYYPKNTTCKPENTVIKVDDIALFQLRNQGKIAANS
                     KEGTITLKCDNLFGDKKQASRNMVVYLSSSDLVKGSNTILRGKTDNGVGFVLDLTEPP
                     KGTEAAIKISANGDQGAATSLWKTDKPGVSLNSNIINIPVMASYYVYDEKKVKSGALE
                     ATALINVKYD"
     gene            complement(5002..5340)
                     /gene="rcnB"
                     /locus_tag="ACHOUK_004253"
     CDS             complement(5002..5340)
                     /gene="rcnB"
                     /locus_tag="ACHOUK_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFP9899583.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHYKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(5558..6415)
                     /locus_tag="ACHOUK_004254"
     CDS             complement(5558..6415)
                     /locus_tag="ACHOUK_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFP9899584.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWRGERNWLENMHEHHHHDHEHHQDHDHDHDHDHDHDHEHHHHHEHGDNEEY
                     QDAHARAHANDIKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGA
                     TLVVSFSIGLALTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMG
                     VHGFMGIMR"
     gene            6536..6808
                     /gene="rcnR"
                     /locus_tag="ACHOUK_004255"
     CDS             6536..6808
                     /gene="rcnR"
                     /locus_tag="ACHOUK_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFP9899585.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            complement(6918..7232)
                     /locus_tag="ACHOUK_004256"
     CDS             complement(6918..7232)
                     /locus_tag="ACHOUK_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HFP9899586.1"
                     /translation="MKKLLVTVIFVAMMPNAFALSHGDSATLKEGTFNCKKLTDFYEM
                     ISYIQDNDQQAMLSLITSNKCRVLDESMTVEIQSVDDKGFVSFITPGGHGGWAVKQFF
                     EN"
     gene            complement(7242..7589)
                     /locus_tag="ACHOUK_004257"
     CDS             complement(7242..7589)
                     /locus_tag="ACHOUK_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899587.1"
                     /translation="MKSKIFAALLLLPLSTYASSYKCDALQQGQIGSNGKTVTAQSSI
                     DIDGKQMHFSLNGVSWDLSYIGEKQIAKMYSTESGNVSVSFIDANPIIFVLHLVNDDG
                     RKGTYNISECSEL"
BASE COUNT         2345 a         1541 c         1879 g         2314 t
ORIGIN      
        1 tttgatgtga gaaatttgtt tccaagagat acagactctt aacaaaacaa aataaactta
       61 ttaaggaatg catcatgaaa cattcaatta ttgctgtcgc tgtcttatct tctgtattta
      121 tgagtgctgg tattttcgct gctgaagtag ataccggaac tttaaccatt aaaggtaata
      181 ttgcagaatc accatgtaca tttgtcgctg gcggtgattc agtcagtatt aatatgccga
      241 ctgtacccac cactgtattt gatggtaaag cgaaatattc aacttatgat ggcgcggttg
      301 gtgtaaccag cagtatgctg aaaattactt gtccgaagac tattgatggc gtaaaacttt
      361 cgttgatcac caacgagaaa attcttggta atgataaggc aatatccagt agtaatgaca
      421 cagttggata ctatctctat ttgggagata gcagtgacgt actggatatt tccgcaccat
      481 ttaatattga aagttataaa actgctgatg gacaatatgc aataccgttt aaggcaaaat
      541 atctgaagtt gactgacagt ggtgttgcat ctggtacagt taactcttca ttggttatgc
      601 gtgttgccca ggattaaata tttaaaataa tcatcttgtt ttttcagtaa gttaaaatgg
      661 ccgccatatt catggcggcc agcataaata taagaggcat taagatgaaa gggttattat
      721 ctttactcat ctcttctatg atattccccg tgcatgccgg aatggttatt ttcgggaccc
      781 gtatcattta cccggcagag cagaaagagg tcatggtaca actgatgaat cagggaaacc
      841 gctcttcgct gatgcaggcg tggattgatg atggcgacac gtctctgccg ccagaaaaga
      901 ttcaggttcc tttcatgtta acgccaccag tagtgaaagt agcggccaat tctgggcaac
      961 aaataaaaat taaaatcatg cctaataaat tacccagtaa taaggaaagt attttttatc
     1021 ttaatgttct ggatattcca ccgaatagcc cagaagatga aggaaagaat gcgttaaaat
     1081 ttgccatgca aaacagaatc aagttgtttt accgacctgc tggtattgct ccggtaaata
     1141 aaacgacttt taaaaaatta cgagtgagtc gtagcggtaa taacgtagtc ataaaaaatg
     1201 actcgccaaa ttgggtaacg atttctgatg ttaaggctaa taacgtcaaa gtgaattatg
     1261 aaacaatcat gattgcacca caagaaagtc agaatgttag tgtaaaaaat aataatgcga
     1321 atggctggca actgaccatt atcgatgatc acggcaacta tattagtgac aaaatttaat
     1381 tgcaggagct gcctatgttg agaatgaccc cgcttgcatc agcaatagtc gcgttattgc
     1441 tcggcattga agcacatgca gcagaagaaa cctttgacac ccattttatg atgggaggaa
     1501 tgaaaggtga gcaagtaaca aatttgcgtc ttgatgataa tcagccttta cctgggcagt
     1561 acgacattga tatttatgtc aataaacaat ggcgtggaaa atatgagatt atcgtcaaag
     1621 ataacccaca tgaaacttgc ctgacgcgtg aaattgtaaa gcggttaggt ataaatagcg
     1681 ataattttgc acgcgaaaat cagtgtttaa catttgagca acttgttcag ggcggaagct
     1741 actcctggga tattgggatt tttcgtttgg atctcgctgt tccgcaagcc tgggttgaag
     1801 agctggaaaa tggttacgtt ccgccagaaa actgggagcg gggaatcaat gcgttttata
     1861 cgtcttatta cgtaagtcag tattacagtg actataaagc ctcgggcaat agtaagagca
     1921 cttatgtgcg ttttaacagt gggctaaatt tgctgggatg gcagctgcat tcagatgcca
     1981 gttttagcaa aacggataat aatcctggtg agtggaaaag caacgcgctg tatctggagc
     2041 atggattttc tcaaattctc ggtacgctgc gtattggtga tatgtacacc tcagccgata
     2101 tatttgattc cgttcgcttc accggggttc ggttatttcg tgatatgcag atgttgccaa
     2161 actcgaagca aaattttacc ccacgggtac aggggattgc ccagagtaac gcgttggtaa
     2221 ctattgaaca gaacggtttt gtcgtttatc agaaagaggt tccaccaggt ccgttttcga
     2281 ttagtgattt gcagttagcg ggcggagggg cggatcttga tgttagtgtt aaggaagccg
     2341 atggttctgt gactacgtat ctggtgcctt atgcggcagt acctaatatg ctgcaacctg
     2401 gtgtatcaaa atatgatttt gcggcaggcc gtagtcatat tgaaggtgcg agcaagcaaa
     2461 gtgattttgt ccaggcgggt tatcagtatg gttttaataa tttattgacg ctgtatggcg
     2521 gcacgatggt tgctaataat tactatgcct ttaccctcgg aacaggttgg aacacgcgta
     2581 ttggcgcaat ttcagtcgat gcaactaaat cgcacagtaa gcaagacaat ggtgatgtgt
     2641 ttgacggaca aagttatcaa attgcctata acaaattttt gagccaaaca tcaacacgtt
     2701 ttggtctggc ggcctggcgt tattcgtcgc gtgattaccg cacatttaac gattatgtgt
     2761 gggcaaataa taaagataat tatcgtcgtg ataaaaacga tgtctatgat attgccgatt
     2821 attaccagaa tgattttggt cgcaaaaata gcttttccgc caatatgagt cagtcattgc
     2881 cagaaggctg gggatctgtg tcattaagta ctttatggcg tgattactgg gggcgaagcg
     2941 gcagtagtaa agattatcaa ctgagttatt ccaataactg gcggcggata agctataccc
     3001 tcgcggcaag ccaggcgtat gacgagaacc acgccgaaga gaaacgcttt aatattttta
     3061 tatcgattcc ctttgactgg ggcgatgacg ttacgacgcc gcgacggcaa atatatatgt
     3121 ctaactcaac gacctttgat gatcagggtt ttgcttcaaa caataccgga ttatcgggaa
     3181 ccgttgggaa ccgcgatcag tttaactatg gtatcaactt gagccatcaa catcagggaa
     3241 atgaaacgac agctggggcc aatttgacct ggaccgcacc ggccgcaaca gtgaatggca
     3301 gctatagtca gtcgagtact tatcgacagg tcggagccag tgtttcaggg ggactggttg
     3361 cctggtctgg tggcgttaat ctggcgaacc gtctttccga aacgtttgct gtgatgcatg
     3421 cgccgggaat caaagatgct tatgtcaatg ggcaaaaata tcgtacaaca aactgtaatg
     3481 gtgtggtggt gtacgacgga ctgacacctt atcgggaaaa tcacctgatg atggatgtgt
     3541 cgcaaagcga tagcgaaaca gaattacgcg ggaaccgtaa aatgaccgcc ccttatcgcg
     3601 gcgcggtcgt tctggttgat tttgataccg atcagcgtaa gccctggttt ataaaagcat
     3661 taagatccga tggacagcca ttaacgtttg gttatgaagt caatgatatg catggtcata
     3721 acattggtgt tgtcggccag ggcagtcaga tatttattcg caccaatgaa ataccgccag
     3781 cggttaatgt ggcaattgat aagcaacaag ggctttcatg cacaatcacc ttcggtaaag
     3841 agattgatga aagtaaaaat tatatctgtc ggtgagaggt gaaatatgat gttcagaaat
     3901 agaatattac taatatttat attgtgggct aattttacct gggctgggtg tcgtactact
     3961 gcatcattaa atattacaga tggtattaat gttggggaga ttttagcgaa tgaaacttcc
     4021 tttagtaaaa gtgtcgtgtt tactgggata tcttgtgata cgagcacgga taaaatagtt
     4081 tataaaaata tccaaagtga ttgggttgaa gttgggcctt ttggtaatgg cgaaaaatta
     4141 aaggttaaaa tagagtcttt aggtaaaacc agcgacacaa ttgggaaatc cagcaatgcg
     4201 caggcagtat taccttatgt ggttaaaata gccagaggca cacctgattt tactggagaa
     4261 agaaaatcta cctggtttat ttcagatacc gtgattgcaa atattggcgg tgagtcatcg
     4321 tcatccatcg atttttggtt gggtatttgt aaggcattga agtttaactg gtgtgtgaat
     4381 tatctcacca gcaaactggc gggggataca tttacgcttg ggttaaatat ttcctattat
     4441 cctaaaaata cgacctgtaa gcctgaaaac accgttataa aagtagatga tatcgccttg
     4501 ttccagctca gaaatcaggg aaagattgcg gcgaacagta aggaaggaac aattacgttg
     4561 aaatgtgata atcttttcgg cgacaaaaaa caagcatcgc ggaatatggt tgtatatctt
     4621 tctagcagtg acttagttaa aggaagtaat actattttgc gtggtaaaac agataatggt
     4681 gtagggtttg tgttggatct aacagaacca ccaaaaggga ctgaggctgc cattaaaatt
     4741 tcggccaacg gcgatcaggg cgcggcgaca tcattatgga aaacagataa accaggagtt
     4801 tcattaaata gcaacattat taatatacca gtcatggcca gttactatgt atatgatgaa
     4861 aaaaaagtta aatctggcgc actggaagca accgcattaa tcaacgtgaa atacgattaa
     4921 taacaagcaa gatgagcaag tggctaataa taagaaataa taaggtgtct gcatttcccc
     4981 ctctctccga gggggctttt tttagcgatg ataaaaaatc tcaccgtcgt aggctttaat
     5041 gattttaccg tcggtgtcgc tgatcaacac gtacgcgcca cccatatagg tccagtgcgt
     5101 cccggcatcc ggcgcgggca ggttacgcaa ctgccactgc ttaatgttgt actcatcggt
     5161 acggtacatc tctggtacgg tgtcccctgg tttgtaatgc ttaaagtcag caatgaaact
     5221 gcttaactca tacttagaaa ttcccgaggt attggtcgaa cccgcggtgg ctggtgcggc
     5281 ccaggcggca ctggtaacca gcaaaagcgc acccagcaac atcttatttt taatggtcat
     5341 aaactctcca ctttggggat ctgtaatatc agtcgcatat ctttcctgcc atacaatata
     5401 actgacaagc gatattttgt tagtgatccc atgatttcgt aaaaagtgta aatgaaagga
     5461 tgtagcaaaa gcatcctcat tgagcgagaa gtgagcggaa taagctgatt taagaatcat
     5521 cctgggctaa aaggatagcg aagcccaggt gattgtttta tctcataata cccataaagc
     5581 catgcactcc catatacacc ccgactaaac caattaacag actggaaaaa tagggggcgc
     5641 gtttagcgag agtgttaaat ccgctccagc gttttgcaac ttgttgaaca ctgattgccg
     5701 ctccaacgcc tactgtaacc agcgttagtg ccagaccgat gctgaaactg acgaccagtg
     5761 ttgcgcccag tgtcagggct ttcaactgaa tgcaaatcaa cagcacggta attgctgccg
     5821 ggcagggaat aaggccacct gttaagccaa ataacaaaat ttgccagttg gtgacctctc
     5881 taccatcaaa gcgtcgttta atgtcattgg catgggctcg tgcatgggca tcctgatact
     5941 cttcgttgtc gccatgttca tgatggtgat gatgttcgtg gtcgtggtcg tggtcgtggt
     6001 cgtggtcgtg gtcgtggtct tgatgatgtt cgtgatcgtg gtgatgatgc tcatgcatat
     6061 tctccagcca gttgcgttcg ccgcgccagg tacgccagaa catccagaaa gcggtgctaa
     6121 taatgatcac tgcggaaatc aactggagcc acggttctgc tgattgagca gtaaagcgct
     6181 tgctgatcac catcccgcca aaggcaatca accagaccac tgcggtatgc gaaatggttg
     6241 ctgccagtcc gagcatcacc gcctgtttaa tggtgccttt gatggcgatg ataaacgccg
     6301 ccatcatcgt ttttgagtgc cctggttcca ggccatgaag cgcaccaagt aagatggcgc
     6361 tggggatgaa gaaccaggcg tttccttgct gaagaagagt tgtaaattcg gtcatgataa
     6421 taattcttag tattaattcg gcaatctgat tctactcccc cccagtacct gatactaccc
     6481 cccagtagat taatagtgct atgatttttc atgttcttgt taaccaggtg ttgccatgtc
     6541 tcatacaatc cgtgataaac agaaactgaa agcgcgtgcc agtaagattc agggccaggt
     6601 cgtggcgctc aagaaaatgc tcgacgagcc gcacgaatgc gctgcagttt tacaacagat
     6661 tgctgctatc cgtggcgcgg taaacggtct gatgcgggaa gtgattaaag gtcatctaac
     6721 ggaacatatc gttcaccagg gggatgagct aaaacgtgaa gaagatctgg atgttgttct
     6781 gaaggtgctg gattcttata tcaaatgaaa gtgcctaata tggccgtagt tgaggcaata
     6841 aatagaggga caagtgacgt taagctgaat tagagtaaat ggcgtgagga cacacgccat
     6901 tcgaggttat agtttgatta attttcgaaa aattgcttca cagcccagcc accatgacca
     6961 cctggagtta tgaatgaaac aaaacctttg tcatctacgc tctggatttc taccgtcatt
     7021 gattcatcta acacgcgaca tttattggat gtaatgagac tcagcatagc ctgttggtca
     7081 ttgtcctgga tgtaactgat catctcgtaa aagtcagtta actttttaca gttaaatgta
     7141 ccttctttaa gtgttgcgct gtctccgtga cttagtgcga atgcatttgg catcattgcc
     7201 acaaagatga cggtaacgag tagttttttc attttagcct cttataattc agaacattca
     7261 ctgatgttgt aagtgccttt tctaccatca tcgtttacga gatgtagcac gaagatgatg
     7321 ggattggcat caatgaaaga tacagagacg ttcccgcttt cggtgctata catctttgca
     7381 atctgttttt caccaatata tgagagatcc caactcactc cgttcaagct aaaatgcatc
     7441 tgtttgccgt caatatcgat ggaggattgt gccgtaacgg ttttcccatt gctgccaatt
     7501 tgaccctgct gaagagcatc acatttatag gagcttgcat aagtagatag aggtagtagc
     7561 agaagtgcag caaaaatttt actcttcata ttttcccttt gcttggattt gggttaccta
     7621 ttttggggta gtcattataa aactattaat tagtaaaacg aacaagtttt catttgttta
     7681 gcaatggtat tgtttaggtt tgaaattatt acgtattttc atagcaatag gttggataaa
     7741 cagcagttca gggaacaagt tgcactatct acatactttt ccgctttcca ttgcatgtta
     7801 ggtaatagat agcctttgcc cgtaagacta aaaatattag aaatgactca ttcgaatacc
     7861 ttctattcac cttaatatag gtttgattat tgtaatcgtt taatgtgtct tatgattttt
     7921 tcctactttt catttgcata atataggggg cacagcatgc tcttgaaatt aattattaaa
     7981 atatttaaat tttacagcac acaactactt ttcgttgtct tatgtgttga acgtacttcg
     8041 agtataggta gggttgtata tatattgcgt gggtagagt
//
LOCUS       DAZKIP010000168         8044 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZKIP010000168 DAZKIP010000000
VERSION     DAZKIP010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8044
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.170"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(68..259)
                     /locus_tag="ACHOUK_004258"
     CDS             complement(68..259)
                     /locus_tag="ACHOUK_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFP9899588.1"
                     /translation="MNTTFALVLTVYLVSGESLELVTGLYGSMKECMAAAAEQKIPGN
                     CYPVDKTTHTNNNEIPAGL"
     gene            complement(256..444)
                     /gene="dicB"
                     /locus_tag="ACHOUK_004259"
     CDS             complement(256..444)
                     /gene="dicB"
                     /locus_tag="ACHOUK_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFP9899589.1"
                     /translation="MEKLSCNASTSELRFEIGVITGDKTFIEDAIKQRKLEQDLLNEV
                     CIPSMLARLDLLQKGYKQ"
     gene            1018..1203
                     /locus_tag="ACHOUK_004260"
     CDS             1018..1203
                     /locus_tag="ACHOUK_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899590.1"
                     /translation="MPNKPCPACNALSGFLEKGGYYIFNCPEHVEFHISKLDSIITKP
                     NQYQSDLLNKELNAARD"
     gene            complement(1390..1779)
                     /locus_tag="ACHOUK_004261"
     CDS             complement(1390..1779)
                     /locus_tag="ACHOUK_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899591.1"
                     /translation="MEFKDLLKEIQEIAAHALHQRLNEVELESATKKYIDNMARNVRD
                     AFTGLYSVSVTNNQNTEETAKRIASVMGFHVEEKYSKKEFWKTTKKLQSENCHLLRQS
                     LLSMRKVIQMTQDYRNCSHYLKNSELS"
     gene            complement(1791..1919)
                     /gene="ydfB"
                     /locus_tag="ACHOUK_004262"
     CDS             complement(1791..1919)
                     /gene="ydfB"
                     /locus_tag="ACHOUK_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
                     /protein_id="HFP9899592.1"
                     /translation="MDFDTIMEKAYEEYFEGLAEGEEALSFSEFKQALSSSAKSND"
     gene            complement(1921..2076)
                     /locus_tag="ACHOUK_004263"
     CDS             complement(1921..2076)
                     /locus_tag="ACHOUK_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFP9899593.1"
                     /translation="MDTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            complement(2354..2641)
                     /locus_tag="ACHOUK_004264"
     CDS             complement(2354..2641)
                     /locus_tag="ACHOUK_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9899594.1"
                     /translation="MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLS
                     EHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIEIVSVTHSRRQFPFSI"
     gene            complement(2641..2832)
                     /locus_tag="ACHOUK_004265"
     CDS             complement(2641..2832)
                     /locus_tag="ACHOUK_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFP9899595.1"
                     /translation="MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDE
                     VMAEMENLIAQIAVTNKSE"
     gene            complement(2860..3261)
                     /locus_tag="ACHOUK_004266"
     CDS             complement(2860..3261)
                     /locus_tag="ACHOUK_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9899596.1"
                     /translation="MMSALEVSMYRISKLLQETGWSQAELARRIGVTQQTVQQWVSGK
                     ATPKASSLDKLVEVSGHPLHWFLLPPEECEQIFTPDTMKIGPRQRELLQAFSAFPEED
                     QEKMLQEIKDKKKSMEETIARWLAAQKSRRA"
     gene            3370..3642
                     /locus_tag="ACHOUK_004267"
     CDS             3370..3642
                     /locus_tag="ACHOUK_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9899597.1"
                     /translation="MLDSTREKIRQKYTQAEIGRYMGVAQQTVWQWFSFGVPPKQVIP
                     LCQLMKWEVTPHEIRPDIYPNPTDGLPVGCKVNTSNAPELIHENQA"
     gene            3626..4051
                     /locus_tag="ACHOUK_004268"
     CDS             3626..4051
                     /locus_tag="ACHOUK_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFP9899598.1"
                     /translation="MKIKHEHIRMAMNAWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEAMARNTQKIFRWVEKDTPDAVKKIQALLPAIEKAMPPPLVARMRSHSSAYFRE
                     LVETKERLVKDIDDFVASAIVLFDQMNRGGPAGNTLAVH"
     gene            complement(4258..4713)
                     /locus_tag="ACHOUK_004269"
     CDS             complement(4258..4713)
                     /locus_tag="ACHOUK_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899599.1"
                     /translation="MYFLKSLYQAHVLNVAATNRWCNSPEMLPDYRAWLRAETYLRLD
                     ILISELQKETASIHNLQGIDAVRILVSRHSALSIIEVRHLSFSELIFLLQPALESANI
                     PPEVIQYPPHVDEQLQDVPYNQRAGLTPCSEAEWDHSLLKKYQDLYNPQ"
     gene            4792..5883
                     /locus_tag="ACHOUK_004270"
     CDS             4792..5883
                     /locus_tag="ACHOUK_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP9899600.1"
                     /translation="MRDYAKVSPRFWLGETGRELRKAGAEAQVVAFYLMTSPHANMLG
                     LYYLPVLYLAHETGLGLEGASKGLKRAVEAGFCSYDHDAEMVWVHEMAAWQVGETLKP
                     GDNRCAGVRNEYASLPENAFLSVFYDRYKTDFHLDVRRNNSRNSVRGFEGAFKGLRSQ
                     EQEQEQEKEQEQDKNTMVHGKKNTTNQAGDVQTVNPGQPAGTTPEADSGAVQQVMTAG
                     SEQSHQLQQPEADSAIQREADRVVPESTGQSVGRVDYPDVFEQVWREYPLRAGANPKK
                     SAFSAWKARLREGVPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGPDRNFENPW
                     LLPVSGTNNQRCVNHISEPDTEIPPGFRG"
     gene            5890..6636
                     /locus_tag="ACHOUK_004271"
     CDS             5890..6636
                     /locus_tag="ACHOUK_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9899601.1"
                     /translation="MKNIAAAGVLERIRRLAPQASVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNHQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTVSDVMSVLHDSYDNGKSGEKFLQE
                     LCSVDLLVLDEIGVQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGGRWVTFNWDSWRPNVSNMRVVK"
     gene            6658..7428
                     /locus_tag="ACHOUK_004272"
     CDS             6658..7428
                     /locus_tag="ACHOUK_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFP9899602.1"
                     /translation="METVSDALKALKKASSHVVAARLGISREEAVNELWELKINGVVD
                     KTGHTWFLAGEGESRVTEERPVKSEAQDMLTGEVEQKVTADMMIEFIGQDGAKTCEEL
                     AGKFGVSTRKVASTLAVVTATGRLARVNQNGKFRYCMPGDNLPAEPKAALVTESDGKA
                     FPQPAGAALPVREAATQEEIKTETVADIVQPLPSFTETQADELIFPSLRRANLALRRA
                     KSDVQKWERVCAALRELNKHRDIVRQITDSSRRVVSEK"
     gene            7444..7857
                     /locus_tag="ACHOUK_004273"
     CDS             7444..7857
                     /locus_tag="ACHOUK_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFP9899603.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRRSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPVVVDPDLIWSLPDG
                     EIRRYDRQLNIICSECRNSEVMQRVLIFYTGVMME"
BASE COUNT         2147 a         1725 c         2098 g         2074 t
ORIGIN      
        1 agggtgaaca aatccctgcc attgctggca tataaaaatg aaaccggata ttaattacgg
       61 tgctgtttta aagtcctgcc ggtatttcgt tattattagt gtgagtagtt ttatctaccg
      121 gataacagtt accgggaatt ttttgttctg ctgctgcagc catgcattct ttcattgaac
      181 cgtataagcc agtcaccagc tcaagagatt cgccggaaac aagataaact gtcagaacga
      241 gtgcaaatgt tgtattcatt gtttatatcc tttttgcagc aggtccagac gagccagcat
      301 tgaaggaatg catacttcat ttaacaggtc ctgctcgagt tttctctgct taatggcgtc
      361 ttcaataaat gttttgtctc cagtgataac gccaatttcg aaacgaagtt cagacgtgct
      421 ggcattacat gataactttt ccattatcgc gtcctcaaca atgaattttg tgatgcggtg
      481 cctggtgcct ccaggtgacg ttaaccagtt aacaattaac gccggataca gagcattctc
      541 gttactcagc aaatgaccgc tttaccgatt taactgttcc gcgtgcgctg agccgcattc
      601 accgcatcac aaaattcact tttaaaaaag ggcggcagag cagttacgga gtaaaactga
      661 taccgccaaa tgtcaccaga atattgataa cagagggcgt tgcagcgggg ttgtcactta
      721 agcgtatggt caacctgaca acccggtgtc ctcaacgggg aaagaataac cccgccatac
      781 ttaccgccgc gccatttcgc ggattgccac aaccggaagc gcacggtcga attaaattta
      841 acgacggctc taagggagaa ggacttcgcc gtgcactttc gtgttatgcc ctgacttttc
      901 agggatatat cctttcagta aactgtcagt gccggattct tatctgtgtc cggcgcacga
      961 ccacacgtag cagcgtgttg gtctccattt ttaattcagc ccttaatgga ggataaaatg
     1021 ccaaacaaac catgccctgc ctgtaacgct ctttctggct ttttagagaa gggcggttat
     1081 tacattttta actgtccaga acatgttgaa tttcatatat caaaacttga cagtatcatt
     1141 acaaagccaa atcagtatca gtctgattta ctgaataaag agttaaacgc cgcccgagat
     1201 taagggcgta gtggcccatt aaaaatattg caaagaccaa ctattgttga actggattaa
     1261 accagtaatt ctctattgct cttattgtta ccatcggatg attgccgggg gttattttaa
     1321 gttcgggagg aaaaggctct ccatcagcag ccatcattga gtatgtgaaa tccgccgacg
     1381 cagtcccttt catgataatt ccgaattttt caagtaatga gagcagttcc ggtaatcctg
     1441 tgtcatctga ataactttcc tcatagaaag aagcgattgc cgcaggagat gacagttctc
     1501 gctctgcaat ttttttgtag ttttccagaa ttcttttttt gaatactttt cctcgacatg
     1561 aaaacccatc accgaggcaa tccgctttgc agtttcttca gtattctggt tgtttgttac
     1621 cgaaacagag tacaatccgg taaacgcatc gcgcacatta cgagccatat tatcaatgta
     1681 tttcttcgtt gcagattcta attcaacttc gttcagacgt tggtgaagtg catgtgctgc
     1741 aatttcctgg atttctttta gtaaatcttt aaattccatc tttaactccg ttagtcgtta
     1801 gattttgccg aactggaaag cgcctgttta aactcactga agctgagagc ttcctcgcct
     1861 tcggcaaggc cttcgaagta ttcttcgtaa gccttttcca tgattgtgtc gaaatccata
     1921 tcactcacct gagtttcttt ccagccagcg acgggcacca ttttcggttt taaacgtttt
     1981 gcttttggta tacgtcatcg cggtgaatgt accgtcctgg ttgggaaaca cgccgtacac
     2041 cagagattcg ttgttgccaa gatcgatagt atccatgttg acctcatttc cccttaacgc
     2101 cgggtagcgg aacaaaaacc tgctgcatag ttattaaagt tgaaccctgc cgtcatgttc
     2161 atacgcctcg ggctggctac ttaccccctg accactgctt ggtaactcga agtattgccc
     2221 ggcgttctgt ggggcggggt gggtggttgg tgtatgtaat ctacaattaa aaactgtttt
     2281 tgtgtcaaca gtttttaatt gttgttttgg gcaaaaaaat ccctcgaaag agggagtatg
     2341 aaaattgttc aactcagata gagaagggaa attgtcgccg agaatgtgtc acgcttacaa
     2401 tctcaatgct tgaagcagct actctgtaca ggattatgta gttagggtgg gtcacgatct
     2461 ctctcaatcc agaaacccgt tcgcttggtg gatataagta cggatgctca gatagaggta
     2521 ataccgatgt ttcaatgcgt atttttagtc tacgtgctgc cggtgggttc tccttggcga
     2581 tgtaagttat gatctggcgc aaatcatcgc gagcagacgg tagccataaa atgggtaaca
     2641 ttactcgctc ttgttagtta cagcaatttg agcaataaga ttttccattt cagccattac
     2701 ctcgtcatgt ggaattgcgg ggcgagtgtc tgcaaggctt gacgttactt tagtgcgcaa
     2761 ccattcgttg taactgtttt cttgttcggt agtttcgaat tctgaaacta tcggagaaag
     2821 ggctgtaccc atggcataac tcctcttctt gtactgtggt cacgcccggc ggcttttttg
     2881 tgccgccagc cacctagcaa tggtttcttc cattgatttt ttcttgtctt tgatttcttg
     2941 aagcattttt tcttggtctt cctcaggaaa cgcactaaaa gcttggagca gttcgcgttg
     3001 gcgaggacca attttcatcg tgtcaggggt gaaaatttgc tcacactctt caggaggcaa
     3061 taaaaaccaa tgcaatggat gccctgaaac ctcaaccagt ttatccaaac ttgaggcttt
     3121 aggtgtagcc ttaccgctga cccattgttg aacagtttgt tgtgtcacac caattctacg
     3181 ggcaagctca gcctggctcc atccagtttc ctgaagaagc ttgctgattc tgtacataga
     3241 tacttctagg gcgctcatca ttattcaatt ttacaggtaa atactgttaa aagcatcaca
     3301 ataaaaaact gttgattgca tacagttttt tattgtaggc tttgcttata gtttttagag
     3361 gagggcaaaa tgctagatag cactcgcgaa aaaattaggc agaaatacac tcaggctgaa
     3421 ataggtcgtt atatgggggt cgctcaacag actgtttggc aatggtttag ctttggcgtt
     3481 cccccaaagc aggtaattcc gttatgccaa ctaatgaagt gggaagttac cccgcatgaa
     3541 atccgcccag atatttatcc taacccaacc gacggtttac ctgttggatg taaggttaac
     3601 acatcaaatg cgccggagtt gattcatgaa aatcaagcat gaacacatcc gcatggcgat
     3661 gaatgcctgg gcgcatccgg acggcgaaaa agtaccggct gcgaaaatta ccaaagcgta
     3721 tttcgagctg ggaatgacgt tcccggaact gtatgacgac agccatccgg aagccatggc
     3781 tcgcaatact cagaaaattt tccgctgggt ggagaaagac actcctgatg cggttaaaaa
     3841 aattcaggcg ttgttaccag cgatcgaaaa agcgatgccg cctccgctgg tggcccgaat
     3901 gcgcagccac agttccgctt attttcggga gttggtagag acgaaggaac ggctggtgaa
     3961 agatattgat gatttcgttg catcagcgat cgttctgttc gatcagatga atcgtggtgg
     4021 cccggcagga aacactctgg ctgtgcatta attgggtaat aaatatgagt aatgacaaaa
     4081 aattgacact gagcgtttac gaaaacagtc cgcacatctg gcgtggcggt ttatctgatg
     4141 tggagctggc agagtggttg atacataaag ctaatgcgct gctctggcgt ttgtcagcca
     4201 gagaacagcg caaggaaacc agaataaagc tggctgatgc agaagcgtgt gccgggctta
     4261 ttgaggatta tacaaatctt ggtatttctt cagcagagag tgatcccatt cagcctctga
     4321 gcagggagtc aatccagcac gctggttgta tggcacatct tgtaactgct cgtcaacatg
     4381 aggtgggtat tggatcactt ccggcgggat attcgctgat tccagagctg gttgaagcaa
     4441 gaaaatcagt tcagaaaaag agagatgacg cacttcaatt attgagagag cactatggcg
     4501 cgataccaga atgcgaacag cgtcgatacc ctgaaggtta tgaatggatg cagtctcttt
     4561 ttgaagttcg ctaatcaata tgtcgagacg aaggtatgtt tcggcgcgca gccaggctct
     4621 gtaatccggg agcatttcgg ggctgttaca ccagcggttt gttgctgcaa catttaatac
     4681 atgagcctga taaaggcttt tcaaaaaata catgtcgaac ctcctctggt tctgtcgatt
     4741 gggaaccaca gattatatcc ggaggaaggt tcggcaccag atgaggtagc catgcgtgat
     4801 tacgcaaaag tttctccgcg attctggctg ggagaaacgg ggagagaact tagaaaggcg
     4861 ggtgcagaag cgcaagttgt tgctttttac ctgatgacat cccctcacgc aaatatgctg
     4921 ggtttgtatt acctgccagt tttatacctt gctcatgaaa ccgggcttgg tctggaaggg
     4981 gcttcaaagg ggcttaaaag ggctgttgaa gctggttttt gtagctatga ccatgatgca
     5041 gagatggtct gggtccatga aatggcagcc tggcaggttg gggaaacgtt gaagcctggc
     5101 gataaccgtt gtgcaggtgt caggaatgag tatgcatcat tacctgaaaa cgcttttctg
     5161 tcagtgtttt acgacagata taaaacggat ttccatctgg atgtgaggcg gaataatagc
     5221 cgaaattcgg taaggggctt cgaaggggct tttaaggggc ttcgaagcca agaacaggaa
     5281 caggagcagg agaaagaaca ggaacaggac aaaaacacta tggttcatgg caaaaaaaac
     5341 accacgaacc aggcagggga tgttcagacc gtcaatcctg gtcagccagc aggcacgaca
     5401 ccggaagccg attcgggcgc tgtgcagcag gtgatgaccg cagggtcgga gcaatcacac
     5461 caactgcagc agcctgaagc cgattccgcc attcagcggg aagccgatcg ggtagtcccg
     5521 gaaagcaccg ggcagtctgt gggacgagtg gattatccgg atgtgttcga acaggtctgg
     5581 cgggaatacc cgttgcgtgc tggggcaaac ccgaagaaat ccgctttcag tgcctggaag
     5641 gccagattgc gcgagggggt gccaccagag accatgctgg atggtgtgag gcgttacgcg
     5701 agatacctgg cggcgaccgg gaaagcggga acggaatttg ttcagcgagc gacgacgttt
     5761 tttgggccgg accggaattt tgaaaacccc tggttgctcc cggtaagcgg cacgaacaac
     5821 cagcgttgtg tgaatcatat ttctgaaccg gataccgaaa ttccgccggg attcaggggg
     5881 tgatgtggca tgaaaaacat tgcggcagcc ggggttcttg aacgtattcg cagacttgca
     5941 ccacaggcgt cggttccacc gtaccggacg gtggaggagt ggcgggaatg gcaacttgct
     6001 gaaggacgaa aacgcagcga ggagattaac cgccagaatc accagttgcg ggtggaaaaa
     6061 atcctgaatc gttcgggcat ccagcctctg cacagcaaat gctcgtttgc gaattatcag
     6121 gtgcagaacg acgggcaaaa atacgcgctg agccaggcca aatccatagc tgacgaactg
     6181 atgaccgggt gcacgaattt tgtgttcagc ggtaaaaccg gcaccgggaa aaatcacctt
     6241 gcagcggcga tgggtaaccg gctgatggcg aaggggcgca gcgtgattat cgtcaccgtg
     6301 tctgatgtca tgagcgtgtt gcatgacagc tacgacaacg gcaaatccgg ggaaaaattt
     6361 ttacaggagc tttgcagtgt tgatttgctg gtcctggatg aaataggcgt tcagcgggag
     6421 acgaaaaacg agcaggtggt attacaccag ataattgatc gccggacagc atcactgtgc
     6481 agtgtcggga tgttaacaaa cctgaatcat gccgcaatga gtacacttct tggtgagagg
     6541 attatggacc gcatgaccat gaacggtggt cgatgggtga cgtttaactg ggatagctgg
     6601 cgtccaaatg tcagcaatat gagggttgtg aagtaatttt gtccggagga aattttaatg
     6661 gaaaccgtat ctgacgcact gaaagcactg aaaaaagcct cttcacatgt ggtggcagct
     6721 cgccttggaa tcagtcgtga agaggctgtc aacgagctgt gggaactcaa aataaatggc
     6781 gtcgttgata aaactggtca cacctggttt ctggctggcg aaggtgaatc ccgggtaacc
     6841 gaagagcggc cagtaaaatc tgaagcacag gatatgctga ccggggaggt cgaacaaaaa
     6901 gttaccgcag acatgatgat tgagtttatc ggtcaggatg gggctaaaac gtgtgaggaa
     6961 ctggcgggta agttcggtgt cagtactcgc aaggttgctt ccacgctggc ggtggtaacc
     7021 gcaacggggc ggctggcacg cgttaatcag aacggtaaat ttcgttactg catgccgggc
     7081 gataatttac cagcagagcc gaaagccgcg ctggtaacgg aaagtgatgg taaggccttt
     7141 cctcagccag caggtgctgc gttaccagtc cgggaagccg caacacagga agaaattaaa
     7201 acagaaactg tggcggacat tgtgcagccg ttgccatcgt ttaccgaaac gcaagcagat
     7261 gagctgattt ttccgtccct tcgcagggca aacctggcgc tgcgcagggc gaaaagtgat
     7321 gttcagaagt gggagcgagt ctgcgccgcg ctgcgggagc tgaacaagca ccgggatatt
     7381 gttcgacaga ttactgattc ttcccgccgt gttgtatcgg aaaagtgatt gccggaggcg
     7441 cttatggcaa aagtatttac acaggaagag cgggaaaaaa ttaaagggca ggttgttgag
     7501 ctagtacgcc ggagtgggcg cgagacgtta cggcaactgg aagccaagac aggtgcgaca
     7561 agatatctga tgagcgttct tgccagagag ctggttgcca gtggcgatgt atacaactct
     7621 ggttacgggt tattcccatc tgaacaggct cgtaaggact ggcaaaacgc ccgcaaaaaa
     7681 ctatcgaggg caaaactgaa gaaaccggtt gtggttgatc ctgaccttat ctggtcatta
     7741 ccagacggag aaatacgccg ctatgacagg caattgaaca taatttgtag tgagtgccgt
     7801 aacagtgaag tgatgcagcg agttttgata ttttacacag gagtaatgat ggaatagtga
     7861 aaataacatg aaacctttgg attctggtgt ttcagtggat gggaaaagag cagatacata
     7921 gaaaatgaat agcaataatt cacagtctgg atgttgtttg tatgcttata aaatgatcct
     7981 agtatgcatt gccggagttg aatcagctcc aatgaagaaa ctgagaggag tatttagaag
     8041 atag
//
LOCUS       DAZKIP010000169         7892 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZKIP010000169 DAZKIP010000000
VERSION     DAZKIP010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.171"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            1..144
                     /locus_tag="ACHOUK_004274"
                     /pseudo
     CDS             1..144
                     /locus_tag="ACHOUK_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            289..7515
                     /locus_tag="ACHOUK_004275"
     CDS             289..7515
                     /locus_tag="ACHOUK_004275"
                     /inference="COORDINATES: protein motif:HMM:NF024728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HFP9899604.1"
                     /translation="MESAGRLDISAGSLNNHQGTVVSDGLSVTLDGALDNTSGRLLSQ
                     KTLSVSGSELVSDDGLIQSGSDMTLDVQDGVLSNRNTKTRGGISSAGTLTVRAGMLNN
                     QQGFMVGQKDMTLNAGTLDNRQGVLGSQASLQISSGTLMNQKGALKAGTDMLLSGGDV
                     SNQEGTLAAGRDLNAHLNVLENQQGTVVSNGNSRLDVTRSDNQGGRLVAQQSLTLSST
                     DIINDASGLIQSGASLNLRADTLSNRNSGDRGGVISQGSMTLNAGTLDSTAGVLLSGD
                     ALSLTAGVVNNTSGQVVANGLLGWNSQALNNQSGLIQGKGISINTAGQTLDNRGGTLN
                     SLQELTVSTGAMDNRSGTVGAKTTADLSTTSLDNREGGRLVSEGELRLHTGGLQNSHG
                     QIQSVGDILFDSVRGVVDNVSGLIRSGSAITLNALQFINRHTQNTGQGLEAQTIHITT
                     QDLDNQEGSILADRALTVMADRTLSNNDGVLSSGATLSVSGRQLAFSNRDGVVKAGQS
                     VSVDAGQLGGDGKLLSLGDMTLKSNTTFSNSGQTIANGNLTLSVNGDVSNTGSLLAGS
                     RLDLNSIRLENTEKGEISAGQTWLNVTDTLLNRGLIDGKYTHLQANTLTNSGTGRIYG
                     DAVGVSAATFNNLDENGVAAVLAGRERVDLGVQTLNNRTHSLIYSAGDMHTGGMLDAN
                     GAATGKAGVLNNHSATIEAAGYLVLSAGQINNVNDHFTTERVVVSTEKVTEYQLSGSD
                     KRWSAGEPGVYVDNDSSNSLKKLHTPEGARDKFTQYDYTRTVEETRVKESDPGKILSG
                     AGMTIVADKLFNDKSQVVAGGKLTIPSGNVENVSVSGEQHVTDKGTSTYYYRIKKKGT
                     DKQGESISPYEPPTAIQTITLKPGELTSHGQVQGSQVTLSPLKPQGTDVQTGLTGNVD
                     ATVAGTDRIPLRPVVSAGEPVILLPGQQFEVSAPQGSIHVAGPDTRLPDSSLFKTNPA
                     VNVPYLVETDPRFTNQKTWLGSDYMQKAFSQNGDNMLKRLGDGFYEQRLIREQVVALT
                     GQRYLDGYSNDEEQFKALMDAGIAFGKQYNLTPGVALTAEQMALLTGDIVWLVNTTVT
                     LPDGSTQTVQVPQVYARVKPGDVNSAGALIAGRDMVMKLDGDLFNSGKLAGKQTVQLS
                     AENIHNQAGTIQGANVSLTARTDINSTGGLLQATDSLLAMAGRDINLTTTTRTAQSDA
                     GQNHFERTSIDSVAGVYVQNDQGRLVLQAGRDMNLTAATVVNQGKDSLTQLSAGRDMT
                     LSTVTTSAQDNITWDKNNRLSQGVTQSTGSTLAGNGDVTLTAGRDMTSQAASLSAQKG
                     LALMAGHDVTLTGAQNTSSLDEYHKVTGSSGMLSKTTTTTHDVTDRRTMTGSELNGDT
                     VSIGAGHNLNVTGSSVAGDNRVSLAAGNNLNIGTLTESNRETHLKQEKKSGLMSSGGV
                     GFSVGSQSLKVTDTATDTTQKGSTVGSVHGDVSLQAGNRLTVNGSDLIAGRDMALSGK
                     EVSITAATDQHVQTHTVEQKTSGLTLALSGTAGSALNTTVETVQAAKSAGNSRLEALQ
                     GVKAALSGAQAVQAGRLADAQGADAGNNNTVGISLSYGSQSSKSEQQSEQTVAKGSTL
                     TAGNNLSIQATGSGVKGVDGDLTIQGSQIKAGNNVLLQANRDVNLVSAENTSKLEGKN
                     TSSGGSVGVGVGVGSGGWGISVSASANQGKGSEKGNGTTHTETTVDAGKQLTIISGRD
                     TTLTGAQAGGETVKVDAGRHLTLTSEQDSDRYDSKQQNASAGGSFTFGSMSGSASVSL
                     SRDKMHSNYDSVQEQTGIFAGRGGFDVTTGQHTQLNGAVIASTATADKNRLDTGTLGF
                     SDTENRADFKTEHQSAGLSTGGSVAGNFLGNMANNLLVGANHEGHADSTTQSAVSAGN
                     ITIRDTQSQKQDVADLNRDAAHANQTLSPIFDREKEQQRLQQAQLIGEIGNQVADIAR
                     TEGQIAGEKAKRDPAALNQARAELEAAGKPFTEQDVAQRAYNNGMAASGFGTGGKYQQ
                     AIQAATAAVQGLAGGNLSAALAGGAAPYLAEVVKTMTTDPVTGEVNKAANVTAHAVVN
                     AALAVAQGNNALAGAAGAATGEVVGMIATQMYGKPVSELSEAEKQTVSTLATVAAGLA
                     GGLVGDSGGAAVAGAQSGKTTVENNYLSSKQIDAWSAEMKSCQAKGGDCGGIIKKYEE
                     LSTAQQKQLISDCAASPATCQQKYGDVLTDSLAVKQAIDRALGEDIPIKMVYDLTATF
                     AQQMQAEGVVATNKVSEALQEEYGLDEVQAGIVASAAASAFGGISKVKGNSQPKIEQI
                     LKPEKNWETARNKALDLVGNLGADSKPVIGRLEVSAGNGKVIGRQSSDGKVGWRVDYD
                     PEKGTHINIWDYSQGKGPGKAVKQVIPFEGNEKSFETILKQLNR"
     gene            7521..>7892
                     /locus_tag="ACHOUK_004276"
     CDS             7521..>7892
                     /locus_tag="ACHOUK_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899605.1"
                     /translation="MTLFDECREALSADFNIVEGLAQQEALGILNKYPLAKGSVTWAE
                     IWHSDYESFDELLCANSVKNDDMFVFADDASIPVFRSNLRLIAENIYDVTALSPKLFI
                     FNDEVIIQPLFPTDMFRLGIKK"
BASE COUNT         2054 a         1883 c         2457 g         1498 t
ORIGIN      
        1 ctgccgggaa gcgtggataa ccgggaaggg ctgcttgcgg caaatggcgc ggtgcgtctg
       61 gatgcactga gccttgataa tcgcaagggg aaagtgcagg cggcgcagtc aggcagtctg
      121 caggtgaaga caaccggtgc cgtggataac cagcagggac gtctgcttgc cagcagtgac
      181 atcctgatta atacacaggc gctgaataac gacaacggac tgatttcagc tgctgccgga
      241 acgggcagga taaaaacaca gcagtctgtc agcaatacgg agggacgaat ggagtccgcc
      301 ggcaggctgg atatttcggc agggagtctt aataaccacc agggtacggt ggtgtctgac
      361 gggctgtccg tgacgctgga tggtgcgctg gataatacgt ccggcagact gctttcacag
      421 aaaacgctgt ctgtgtcagg cagcgaactg gtctctgatg atggtctgat tcagtccggc
      481 agtgacatga cgctggatgt acaggacggg gtacttagta accggaacac gaaaacacgg
      541 ggtggtatca gcagcgccgg taccctgaca gtgcgggctg gtatgctgaa taaccagcag
      601 ggatttatgg tgggtcagaa ggatatgacc ctgaacgccg gaacgcttga taaccgtcag
      661 ggcgtgctgg ggagtcaggc gtcattacag atatcgtcag gtacgctgat gaaccagaaa
      721 ggcgcactga aggccgggac ggatatgttg ctgagtggtg gtgacgtcag taatcaggaa
      781 gggacgcttg ccgccggcag agacctgaat gcgcacctga atgtgctgga aaatcagcag
      841 gggacggtgg tcagcaacgg taacagcagg ctggatgtca cacgctctga taatcagggc
      901 ggcagactgg tggcacagca gtcactgacc ctttcctcga cggatatcat caacgatgcc
      961 agcggcctga tacagagcgg tgcatcgctg aatctccgtg ctgacacact gagtaaccgt
     1021 aacagtggtg accggggcgg ggttatcagt cagggttcga tgacgctgaa tgccggcaca
     1081 ctggacagca ctgccggtgt gcttttgtcg ggagatgctc tgtccctgac tgcaggcgtg
     1141 gttaacaata catccggtca ggtggtggca aacggtctgc tcggctggaa cagccaggcg
     1201 ctgaacaatc agtccggtct tattcagggg aaaggcatca gcattaatac cgcaggccag
     1261 acgctggata acagaggcgg tacgctgaac agcctgcagg aactgactgt cagtaccgga
     1321 gcaatggata accggagcgg gaccgtgggg gcgaaaacga cagctgacct gagcaccacc
     1381 tcgctggata accgtgaggg tggccggctg gtcagtgaag gtgaactccg tctgcatacg
     1441 ggaggcctgc agaacagcca cggacagata cagtctgtgg gcgacattct gttcgacagt
     1501 gtacggggcg ttgtggacaa cgtctctggt ctgattcgca gtggttctgc cattacgctg
     1561 aacgccctgc agtttatcaa tcgccacacg cagaataccg gccaggggct ggaagcgcag
     1621 acaatacaca tcacgacgca ggaccttgat aaccaggagg gcagtattct ggcagacagg
     1681 gcgcttacgg ttatggcaga caggacgctc agtaataatg atggcgtgct ctcttccggc
     1741 gcgacattgt ctgttagtgg ccgtcagctg gcgttttcga accgtgacgg cgtggttaag
     1801 gccgggcagt cagtatctgt ggatgccggt cagctcggtg gtgacgggaa actgctttcg
     1861 ctgggcgata tgacgctgaa aagtaacaca acgttcagca acagtggtca gactattgcg
     1921 aacggaaacc ttacgctttc cgttaatggc gatgtctcta ataccggcag cctgctggca
     1981 ggcagccggc ttgacctgaa cagcatccgc cttgaaaaca cagagaaagg tgaaatcagt
     2041 gccgggcaga cctggcttaa cgtgacggac acactgctga accgtgggct gattgacggg
     2101 aaatatacgc atcttcaggc gaacaccctg acgaattccg gtaccgggcg aatctacggc
     2161 gatgctgttg gtgtcagcgc tgccacattt aataatctgg atgaaaatgg cgtggcggcg
     2221 gtgctggccg ggcgtgagcg tgtggactta ggggtacaga ctctgaataa ccggacgcac
     2281 agcctgattt acagtgcagg tgatatgcat actggtggca tgctggatgc gaatggtgcc
     2341 gctacgggga aagccggtgt actgaataat cacagtgcca ctattgaagc cgccggatat
     2401 ctggtactga gtgccggaca aatcaataac gtcaatgacc atttcaccac ggaacgcgtg
     2461 gtggtttcca cggaaaaggt gactgagtat cagctcagcg gctcagataa acgctggagc
     2521 gccggtgaac cgggcgttta tgtggataac gattccagta actcactgaa aaaactgcat
     2581 acaccggaag gcgccagaga taaattcacg cagtacgatt acacccgtac cgtggaagaa
     2641 acccgggtaa aagaatccga cccgggaaaa attctgtccg gtgccggcat gacgattgtg
     2701 gcagacaaac tgtttaatga taagagtcag gttgttgcag gcggtaaact gaccattccg
     2761 tcaggaaacg tggaaaacgt cagtgtcagc ggagagcaac atgttactga caaaggcaca
     2821 tcaacatatt actaccgcat caaaaagaaa gggacggaca aacaggggga aagcatttcc
     2881 ccttacgagc cgcccactgc cattcagacc atcacgctga agccgggaga gctcaccagc
     2941 cacgggcagg ttcagggcag ccaggtcacg ctttctcccc tgaagccaca gggaacggat
     3001 gtacagacag gactgaccgg aaatgtggat gcgacagtag ccggtactga ccggataccg
     3061 cttcgcccgg tggtgtctgc cggtgaaccg gtcattctgt tgccggggca gcagtttgaa
     3121 gtcagcgcac ctcagggcag tatccatgtt gccgggccgg atacccgtct gccggacagc
     3181 agcctgttta aaacaaatcc ggcagtgaat gtgccatatc tggttgaaac ggacccgcgt
     3241 tttacgaacc agaagacgtg gctgggcagt gattacatgc agaaggcgtt ttctcagaac
     3301 ggggataaca tgctcaaacg cctgggggat ggtttttacg aacagcgtct gattcgtgag
     3361 caggttgtcg ctctgaccgg gcaacggtac ctggacggat acagtaatga tgaagagcag
     3421 tttaaggcgt taatggatgc gggtattgca tttggtaagc aatacaacct gacaccaggt
     3481 gtggcgctga ctgctgagca gatggcgtta ctgaccggtg atattgtctg gctggttaac
     3541 accacggtga cactgccgga cggcagcacg cagacggttc aggtacctca ggtgtatgcc
     3601 cgtgtaaaac ccggtgatgt gaacagcgcc ggtgcgctga ttgccggcag ggacatggtg
     3661 atgaagctgg atggtgacct gtttaacagc ggtaaactgg ccgggaagca gaccgtgcag
     3721 ctcagtgcag aaaacattca caaccaggcc ggcaccattc agggagcaaa tgtcagcctg
     3781 acggcccgga cggatattaa cagtaccggc gggctgctgc aggccacgga cagcctgctg
     3841 gcgatggccg ggcgggatat taatctgacc accaccaccc gcacggcaca aagtgacgcc
     3901 ggacagaacc attttgaacg caccagcatc gacagtgtgg cgggcgtgta tgtacagaat
     3961 gaccagggaa gactggtgct gcaggccgga cgggatatga atctgacggc tgcaacggtg
     4021 gtgaatcagg gtaaggacag cctgacgcaa ctcagtgcgg gcagggatat gacgctttcc
     4081 acagtgacca catccgcgca ggacaatatt acctgggaca aaaataatcg tctgtcgcag
     4141 ggcgtcacac agagtacggg cagcacgctt gccggtaacg gtgatgtcac gctgaccgcc
     4201 ggacgggata tgacgtcaca ggcggcatcg ctgtcagctc agaaaggcct ggctctgatg
     4261 gcgggtcatg acgtgacgct gaccggtgca cagaatacca gttcgctgga cgaataccat
     4321 aaagtgaccg gcagcagcgg gatgttatcg aaaaccacca ctaccacgca tgatgtcact
     4381 gaccgccgga ccatgacagg cagtgagctg aacggggata cggtcagtat cggtgccgga
     4441 cataatctga acgtcacggg cagcagtgtg gccggggata accgtgtctc cctggctgcc
     4501 ggaaataacc tgaatatcgg aacgctgaca gaaagcaacc gtgaaacaca cctgaaacag
     4561 gagaaaaaat ccgggctgat gagctccgga ggtgtgggat tcagcgtggg cagccagagt
     4621 ctgaaagtca cggacaccgc aacagacacc acacagaaag gcagcacggt ggggagtgtt
     4681 cacggtgatg tttcccttca ggcaggtaac cggttgaccg tcaacggctc tgacctgatt
     4741 gccggcaggg acatggcact cagtggtaag gaagtcagca ttaccgccgc cacagaccag
     4801 catgtacaga cacatacggt ggagcagaaa acctccggtc tgacgcttgc cctgtccggc
     4861 acagcgggca gcgccctgaa tacgacggtg gagaccgtac aggcggcgaa atctgccgga
     4921 aacagccggc ttgaagccct gcagggggtg aaggccgcgc tctcgggggc tcaggccgtg
     4981 caggctggac gactggcaga cgcgcagggc gccgatgccg gaaataataa cacggtgggc
     5041 atcagcctct cttacggcag ccagtcttcg aaatcagaac agcagtcaga gcagacggtg
     5101 gcgaagggca gtacactgac cgcaggtaat aacctcagta ttcaggccac cggctccggc
     5161 gtgaagggcg tggatggtga cctgacgata cagggcagtc agataaaggc cgggaacaac
     5221 gttctgctgc aggcaaaccg tgacgtgaat ctggtatcag cagaaaacac gtcaaagctg
     5281 gaaggaaaga acacatccag tggtggcagc gtgggtgtcg gtgttggtgt gggctccgga
     5341 ggctggggta tcagtgtttc agccagcgca aaccagggta agggcagtga aaaaggcaac
     5401 ggtaccaccc atacagaaac cacggtggat gcgggaaaac agctgaccat tatcagcggg
     5461 cgggacacca cgcttaccgg cgcgcaggct ggcggtgaaa cggtgaaagt ggatgccggt
     5521 cgccatctga cactgaccag tgagcaggac agtgaccgtt atgacagtaa acagcagaat
     5581 gccagcgccg gaggcagctt cactttcggg tccatgagcg gttcggcgag tgtgagcctc
     5641 agccgggata aaatgcacag taactacgac agtgtgcagg agcagacggg gatttttgcc
     5701 ggcaggggcg ggtttgatgt caccaccgga cagcatacac aactgaacgg tgccgtgatt
     5761 gcgtccacgg caacggcaga taaaaaccgg ctggataccg gcacactggg cttcagtgac
     5821 actgaaaacc gggctgactt taaaacggaa catcagagtg cagggctcag taccggtggc
     5881 agtgttgccg gaaatttcct ggggaacatg gcaaacaatc tgctggtcgg tgcaaatcat
     5941 gaaggccatg cggacagtac cacgcagtcg gcagtgtctg ccgggaatat caccatccgg
     6001 gatacgcaga gccagaaaca ggatgtggca gacctgaacc gtgacgctgc acatgcaaat
     6061 cagacgctgt cccccatctt tgacagggaa aaagagcagc agcgactgca gcaggcgcaa
     6121 ctcattggtg agattggtaa ccaggtggca gatattgcca gaacggaagg acagatagcc
     6181 ggtgagaaag cgaagcggga cccggcagca ctgaatcagg cccgcgcaga gcttgaggca
     6241 gcaggaaaac cgttcacgga acaggatgtg gcacagcggg cctacaataa cggtatggcg
     6301 gcctcaggtt ttggaacggg aggcaaatac cagcaggcga tacaggccgc aacggcagca
     6361 gtacaggggc tggccggcgg taatctgagt gcagccctgg caggtggtgc agcaccgtat
     6421 ctggctgaag tagtgaaaac catgaccaca gacccggtta caggtgaggt gaataaggct
     6481 gcaaatgtga ccgcccatgc cgtggtgaat gccgcactgg cggtggcaca gggaaataat
     6541 gctcttgccg gtgctgcagg tgcagccacc ggcgaggtgg tgggaatgat agccacacag
     6601 atgtacggta agccggtgag tgaactgagc gaggcagaga agcagacggt ctcgacactg
     6661 gcaacggttg ctgccggctt ggccggtggc ctggtgggcg actcaggggg ggctgctgta
     6721 gccggcgcac agtcaggtaa gacgactgtt gagaataact atctgagcag taagcagata
     6781 gatgcttggt ccgctgaaat gaagtcctgt caggcgaaag gcggtgactg cggcggtatt
     6841 atcaaaaagt acgaagagtt gagtacggca cagcaaaagc aactgatcag tgactgtgca
     6901 gccagcccgg caacctgcca gcagaaatac ggtgatgtac tgactgacag tctggcggtg
     6961 aagcaggcaa tagatcgtgc tctgggtgaa gatatcccga tcaaaatggt ctacgatctg
     7021 acggcgactt ttgcgcagca aatgcaggct gaaggggttg tcgcgaccaa caaagtctcg
     7081 gaagcactcc aagaagaata cggtttggat gaagttcagg cgggaatagt cgcgagcgct
     7141 gcggcgtcag catttggggg aattagtaag gttaaaggga attcccagcc taaaattgaa
     7201 caaatcctca agccagagaa aaactgggaa actgctcgaa acaaggcatt ggatcttgta
     7261 ggtaatttag gagcggactc taagccagtt atcggacgtt tggaagtcag cgctggtaat
     7321 gggaaagtca tagggcggca atccagcgac ggaaaagttg gttggcgtgt tgattatgat
     7381 cctgaaaaag gcacacacat caatatatgg gattactcgc agggtaaagg ccctggaaaa
     7441 gcggtgaagc aagtcatacc ttttgaaggc aatgaaaagt cttttgaaac aatattgaaa
     7501 cagctaaaca ggtagagcaa atgactttat ttgatgagtg tcgggaagca ctgagtgctg
     7561 attttaatat agtagagggg ctggctcagc aggaggctct gggcatttta aataagtacc
     7621 ctctcgcgaa aggaagtgtt acttgggcag agatctggca ttctgattat gagagttttg
     7681 atgagctatt gtgtgcaaac tctgtaaaaa acgatgacat gtttgtcttt gcagatgatg
     7741 caagtattcc agtttttagg tcaaatctaa gattaatcgc tgaaaatatc tatgacgtaa
     7801 cagctttatc gcccaaatta ttcattttta atgatgaagt gataatacaa cctttattcc
     7861 caactgatat gttccgttta ggaatcaaga aa
//
LOCUS       DAZKIP010000170         7836 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZKIP010000170 DAZKIP010000000
VERSION     DAZKIP010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.172"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            98..187
                     /gene="kdpF"
                     /locus_tag="ACHOUK_004277"
     CDS             98..187
                     /gene="kdpF"
                     /locus_tag="ACHOUK_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFP9899606.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALIHAEAF"
     gene            187..1860
                     /gene="kdpA"
                     /locus_tag="ACHOUK_004278"
     CDS             187..1860
                     /gene="kdpA"
                     /locus_tag="ACHOUK_004278"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFP9899607.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLAWLINDIPLPGTTGVER
                     VLFSALGVSNHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALVIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            1883..3931
                     /gene="kdpB"
                     /locus_tag="ACHOUK_004279"
     CDS             1883..3931
                     /gene="kdpB"
                     /locus_tag="ACHOUK_004279"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFP9899608.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSVAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVVATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAVTYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            3940..4512
                     /gene="kdpC"
                     /locus_tag="ACHOUK_004280"
     CDS             3940..4512
                     /gene="kdpC"
                     /locus_tag="ACHOUK_004280"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFP9899609.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTDNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPNASTSVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            4505..7189
                     /gene="kdpD"
                     /locus_tag="ACHOUK_004281"
     CDS             4505..7189
                     /gene="kdpD"
                     /locus_tag="ACHOUK_004281"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFP9899610.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RKLALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7186..>7836
                     /gene="kdpE"
                     /locus_tag="ACHOUK_004282"
     CDS             7186..>7836
                     /gene="kdpE"
                     /locus_tag="ACHOUK_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFP9899611.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPSHFITET"
BASE COUNT         1601 a         2066 c         2340 g         1829 t
ORIGIN      
        1 tatacttttt ttacaccccg tccgcagatt tttgcgaaat ctttgcagcc agaattctac
       61 ccttccggta tcacttttag gccactggag gtgcactgtg agtgcaggcg tgatagccgg
      121 cgtgttgctg gtgtttttat tactgggtta tttggtttat gccctgatcc atgcggaggc
      181 attctgatgg ctgcgcaagg gttcttactg attgccacgt ttttactggt attaatggtg
      241 ctggcgcgtc ccttaggtag cgggctagcg tggctgatta atgatattcc tcttcctggt
      301 acgacgggcg ttgaacgcgt actctttagc gcgcttggcg tctctaacca tgagatgaac
      361 tggaagcagt atctctgtgc cattctcggc ctgaacatgc tggggctggc tgtgctgttt
      421 tttatgttgc tcggtcagca ctatctgccg cttaatccac agcagttgcc ggggctgtcg
      481 tgggatctgg cgctgaatac cgccgtcagc tttgtcacga ataccaactg gcaatcttat
      541 agcggtgaaa ccacgttgag ctatttcagc cagatggcgg gcttaacggt gcaaaacttt
      601 ctttctgccg ccagcgggat tgcggtgatt tttgccctca ttcgtgcatt tacccgccag
      661 agcatgagca cactcgggaa tgcctgggtc gatctgctac gcatcacgtt atgggtgctc
      721 gtccctgtgg cgttggtgat cgcactgttt tttattcaac aaggtgcgct gcaaaacttt
      781 ctgccctatc aggcagtgaa taccgttgaa ggagcgcaac agctgttacc aatgggtccg
      841 gtggcttctc aggaagcgat caagatgctc ggcactaacg gcggtggttt ctttaatgcc
      901 aactcgtcgc atccgtttga aaacccaacc gcactgacca acttcgtgca gatgctggcg
      961 atcttcttga tcccaacagc gctgtgcttt gcctttggtg aagtgacggg cgatcgccgt
     1021 caggggcgca tgttgctgtg ggcgatgtca gtgatttttg tcatctgcgt aggcgtggtg
     1081 atgtgggcag aagttcaggg taatcctcat ctgctggcgc tcggcgcaga cagcagcatc
     1141 aatatggaag gtaaagagag ccgtttcggc gtgctggtca gtagcctgtt tgcggtcgtg
     1201 actacggcgg cgtcctgtgg cgcggtgatt gcaatgcatg attcgtttac cgctctcggt
     1261 ggcatggtgc cgatgtggct gatgcaaatt ggtgaagtgg tattcggcgg tgtcggttct
     1321 ggtctttacg gcatgatgct gtttgtcctg ctggcggtgt ttattgccgg gctgatgatt
     1381 ggtcgtacac cggaatatct gggtaaaaaa atcgacgtac gtgagatgaa actgactgca
     1441 ctggcgattc tggtcacccc gacgctggtg ctgatgggcg cggcgctggc aatgatgacc
     1501 gacgccggac gtagcgccat gctcaaccct ggcccgcatg gttttagcga agtgctgtac
     1561 gccgtgtcat ccgccgctaa caacaacggc agcgcctttg ccggattaag cgccaactct
     1621 ccgttctgga actgtttact ggcgttctgc atgtttgtcg gtcgcttcgg ggtgattatc
     1681 ccggtgatgg caattgccgg ttcgctggtg agtaaaaaga gccaacccgc cagctccggc
     1741 acgctgccaa cgcacggccc gctgtttgtt ggcctgttaa tcggcaccgt gttgctggtt
     1801 ggcgcactga cctttatccc tgccctggcg cttggtccgg tggcggaata tctctcctga
     1861 tgatattgag tgagcactga atatgagtcg taaacaactg gcgctattcg aaccaacact
     1921 tgtcgttcag gcgctgaaag aagcggtgaa aaaattaaac ccgcaggcgc aatggcgcaa
     1981 tccggtgatg tttatcgtct ggatcggcag tctgctgacc acctgtatta gcatcgcgat
     2041 ggcaagcggc gcgatgcctg gcaatgcgct gtttagcgtg gccattagcg gctggctgtg
     2101 ggtcaccgta ctgttcgcta atttcgccga ggcgctggca gaaggccgca gtaaagcgca
     2161 ggccaacagc ctgaaagggg tgaaaaaaac ggcctttgcc cgcaagctgc gtgagccgaa
     2221 atatggcgct gcggcggaca aagttcccgc cgatcaactg cgtaaaggcg atatcgtact
     2281 ggtagaagca ggcgatatta tcccctgcga tggtgaagtt attgaaggcg gcgcatcggt
     2341 cgatgaaagc gccatcaccg gggaatcggc accggtgatc cgtgaatccg gcggcgattt
     2401 tgcctccgtc accggcggca cgcgtattct ttctgactgg ctggtgattg agtgtagcgt
     2461 taaccccggc gagacatttc tggatcggat gatcgcgatg gtggaaggcg cacagcgacg
     2521 caaaacgccg aacgagattg ccctgaccat cctgctgatt gccctgacta tcgtcttttt
     2581 actggcaacc gccacgctgt ggccgttttc cgcgtggggc ggtaatgcag tcagcgtaac
     2641 ggtactggtg gcgctgctgg tctgtctgat cccaaccact attggcggcc tgttgtcagc
     2701 gatcggcgtc gccgggatga gtcggatgct gggcgcgaat gtggttgcca ccagcggacg
     2761 tgcagttgaa gcggcaggtg acgttgacgt tctgctgctg gataaaaccg gcaccatcac
     2821 actcggtaac cgtcaggcat cggagtttat ccctgcgcag ggcgtggatg aaaaaacgct
     2881 ggctgacgcc gcacaactgg cttcgctggc tgatgaaacg ccggaaggcc gcagtattgt
     2941 gatcctcgcc aagcagcgtt ttaacctgcg cgagcgcgat gtgcagtcgc tccatgccac
     3001 ctttgtaccg tttactgcgc aaagccggat gagcgggatc aatatcgaca accgcatgat
     3061 ccgtaaaggt tctgtcgatg ccattcgccg ccatgttgag gctaacggtg gtcacttccc
     3121 tgccgatgtt gatcaaaaag tcgatcaggt tgcgcgtcag ggggccacac cgctggtagt
     3181 agtagaaggt tctcgtgtgc tgggcgttat tgcgttgaaa gatatcgtca aaggcggcat
     3241 taaagaacgc ttcgcccaac tgcgcaaaat gggtattaaa acggtgatga ttaccggcga
     3301 taaccgtctg actgccgccg cgatagctgc ggaagcgggc gtcgatgatt ttctcgccga
     3361 agcgacaccg gaggccaagc tggcattgat ccgtcagtat caggcggaag gccgtttggt
     3421 agcaatgacc ggcgacggca ccaacgatgc tccggcgctg gcgcaggcag atgtcgcggt
     3481 ggcgatgaac tccggtactc aggcggcgaa agaagcgggc aatatggtgg atctcgactc
     3541 taacccgacc aagttgatcg aggtggtaca tattggcaaa cagatgctga tgacccgtgg
     3601 ctcgctgacc accttcagca ttgccaacga tgtggcgaaa tacttcgcca ttattccggc
     3661 ggcattcgcg gtaacgtatc cacagttaaa tgcgctgaac atcatgcgcc tgcattcgcc
     3721 cgactccgcc attctcagtg cggtcatttt caacgccttg attatcgtct ttttgattcc
     3781 cctggcgtta aaaggcgtga gttataaacc gcttaccgct tctgccatgt tgcgccgtaa
     3841 cttatggatt tacggtctgg gtgggctgct ggtgccgttt attggtatca aagtcattga
     3901 tttactactg accgtttgcg gtctggtgtg aggtttacca tgcgtggatt acgtccggca
     3961 ttatcgacat ttatctttct gttattgatt actggcggtg tttacccgct gctgaccacc
     4021 gcgctggggc aatggtggtt tccctggcag gccaacggtt cgttgatccg tgaaggtgat
     4081 acggtgcggg gttcggcatt aatcgggcag aattttaccg acaacggcta ttttcatggt
     4141 cgcccgtcgg caacggcaga aatgccttat aatccacagg cttctggcgg gagcaatctg
     4201 gcggtcagta accctgagct ggataaacta atagccgcac gcgttgctgc attacgggcc
     4261 gctaacccga atgccagcac gagcgttccg gttgaactgg tgacggcgtc ggcaagcggg
     4321 ctggacaata acatcacccc gcaagcggcg gcctggcaga tcccacgcgt agcgaaagcg
     4381 cgtaatctca gcgttgaaca gctcacgcaa ctgatcgcaa aatacagcca acaaccactg
     4441 gtgaaatata tcggccagcc ggtcgtcaac attgtcgaac tcaatctggc actggataaa
     4501 cttgatgaat aacgaaccct tacgtcccga ccccgatcgt ctgctggaac aaactgccgc
     4561 gccgcatcgg gggaagctga aagttttctt cggtgcctgc gcaggcgtcg ggaagacctg
     4621 ggcgatgctg gcagaagccc agcgactgcg ggcgcaaggg ctggatattg tggttggcgt
     4681 ggtagaaacc cacgggcgga aagataccgc cgccatgctg gaagggctgg ctgttctgcc
     4741 gccaaaacgc ctggcgtacc gtgggcggca catcagcgag tttgatctcg atgccgccct
     4801 cgcccgccgt ccggcgctga tcttaatgga cgaactggcg cacagtaatg cgccaggttc
     4861 tcgtcatcct aaacgctggc aggatatcga agaactgctg gaagctggca ttgatgtttt
     4921 cacaaccgtc aacgttcagc atctggaaag tctgaatgat gtggtcagcg gcgtcaccgg
     4981 aattcaggta cgggaaaccg tgcccgatcc ttttttcgat gccgccgacg acgtggtgct
     5041 ggtggacttg cccccggacg atctgcgcca gcggctgaaa gaaggcaaag tctatattgc
     5101 cgggcaggcg gagcgcgcca ttgaacattt tttccgcaaa ggcaatctga tcgccctgcg
     5161 caaactggca ctgcgccgta ctgccgatcg cgttgatgag caaatgcgcg cctggcgggg
     5221 gcatcctggc gaagagaaag tctggcacac gcgcgacgcg atccttttat gcatcggcca
     5281 taacaccggc agcgaaaaac tggtccgcgc ggcggcgcgg ctggcgtcac ggctggggag
     5341 cgtctggcac gcggtgtatg ttgaaacccc tgccctgcat cgcttaccgg agaaaaaacg
     5401 tcgggcaatt ctcagcgcct tacgtctggc gcaggagctg ggcgcggaga cggcaacact
     5461 ttctgatcca gcggaagaga aagcggtagt gcgttatgcc cgtgaacata atctcggcaa
     5521 gattattctc ggtcgcccag cctcgcgccg ctggtggcgt cgggaaacgt ttgctgaccg
     5581 actggcgcgc atcgcccccg atctcgatca ggtgctggtc gcgcttgatg aaccacccgc
     5641 ccgcacgatt aacaacgcgc cggatagccg ctcttttaaa gacaagtggc gggtacagat
     5701 tcagggatgc gtggttgccg ccgcgttatg cgccgttatc accttaattg ccatgcagtg
     5761 gctgatggcg tttgatgccg ccaacctggt gatgctgtat ctacttggcg tggtggtggt
     5821 ggcgctattt tatggacgct ggccttcggt ggttgctacc gtcattaatg tggtgagttt
     5881 cgatctcttt tttatcgccc cacgcggcac gctcgccgtc tctgatgtgc aatatctgct
     5941 gaccttcgcg gtgatgttaa ccgtcgggct ggtgatcggg aaccttactg cgggtgtgcg
     6001 ttatcaggcg cgggtagccc gttaccgcga gcaacgcaca cggcacttat atgaaatgtc
     6061 gaaagctctg gcggtgggcc gcagtccgca ggacatcgcc gccaccagcg aacaatttat
     6121 tgcctccacg tttcatgccc gcagtcaggt gttgttgccc gatgacaacg gtaaattgca
     6181 gccgttaaca catccgcaag gaatgacgcc gtgggacgat gccatcgcgc agtggagttt
     6241 tgataaaggc ctgcctgcgg gtgcgggcac cgacacgtta cccggcgtgc cgtaccagat
     6301 tttgccgtta aaaagcggtg agaaaaccta cgggctggtg gtggtggaac cggggaatct
     6361 gcgtcagttg atgatcccgg aacagcagcg cctgctggag acgtttacgc tgttagtcgc
     6421 caatgcgctt gagcggctga cgctgaccgc cagcgaagaa caggcgcgga tggcaagcga
     6481 acgtgaacag atccgcaacg ccctgctggc ggcgctttcg catgatttac gcactccgct
     6541 tacggtgctg tttggtcagg cagaaatctt aacgctcgat ctggcaagcg aaggatcacc
     6601 ccacgcccgc caggccagcg agattcgtca gcatgtgctg aacactaccc gactggtgaa
     6661 taatctactg gatatggcgc gaattcagtc cggcggcttt aatttgaaga aagagtggtt
     6721 aacgctggaa gaagtagtcg gcagcgcgct gcaaatgctg gaaccgggtt tatcgtcgcc
     6781 catcaatctt tctctgccag aaccgctgac cttaatccac gttgacgggc cactctttga
     6841 acgggtgctg attaatctgc tggagaacgc ggtgaaatat gcgggtgcgc aggccgaaat
     6901 tggtatcgat gcccacgttg agggcgaaaa tctacaactg gatgtctggg ataacggccc
     6961 cggtcttccg ccaggccagg agcagacgat atttgataag tttgctcgcg ggaataaaga
     7021 gtcggcggta ccgggggtag ggcttggact ggcaatttgt cgggcgatag tggatgtaca
     7081 cgggggcact attaccgcgt tcaaccggcc ggaaggtggt gcctgttttc gtgttacact
     7141 tccccagcaa actgcccctg aacttgaaga atttcatgag gatatgtgac aaacgttctg
     7201 attgttgaag atgaacaggc tattcgtcgc tttctgcgca cggcgctgga gggcgacggg
     7261 atgcgcgtct atgaggccga aacgctgcaa cgcggcttgc tggaagcggc tacccgtaag
     7321 ccagatttga ttattctcga tctcggcctg cccgatggtg atgggattga gtttatccgc
     7381 gacctgcgcc agtggagcgc ggtgccggtg attgtgcttt ccgcacgcag cgaagagagc
     7441 gacaaaatcg ccgcgctgga tgccggagcg gatgattatc taagtaagcc gtttggcatt
     7501 ggcgaattgc aggcccgtct gcgcgtcgct ttacgccgcc actccgccac cgccgcgccc
     7561 gatccgctgg tgaaattttc cgatgttacc gtcgatttag ccgcccgcgt gattcaccgg
     7621 ggtgaggaag aggtgcatct caccccgatt gagtttcgcc tgctggcagt gctgctcaac
     7681 aacgccggaa aagtgctcac ccagcgccaa ctccttaacc aggtgtgggg gccaaacgcg
     7741 gtcgaacaca gtcattattt gcgtatttat atgggacatc tgcgacaaaa actggaacag
     7801 gatcccgctc gccccagcca tttcattact gaaacc
//
LOCUS       DAZKIP010000171         7777 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZKIP010000171 DAZKIP010000000
VERSION     DAZKIP010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.173"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            56..1389
                     /gene="adeQ"
                     /locus_tag="ACHOUK_004283"
                     /gene_synonym="yicO"
                     /pseudo
     CDS             56..1389
                     /gene="adeQ"
                     /locus_tag="ACHOUK_004283"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
     gene            1442..1894
                     /gene="yicN"
                     /locus_tag="ACHOUK_004284"
     CDS             1442..1894
                     /gene="yicN"
                     /locus_tag="ACHOUK_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFP9899612.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2010..2333)
                     /locus_tag="ACHOUK_004285"
     CDS             complement(2010..2333)
                     /locus_tag="ACHOUK_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9899613.1"
                     /translation="MTAKTKFKSPAFEAIHSAAAGLSSVNAISAETMRTFDKACLTSV
                     QDLQPVEIKALREKLKVSQPVFAHYLNTSVSTVQKWESGAKRPNGISLKLLSIVQKHG
                     LEVLL"
     gene            complement(2317..2670)
                     /locus_tag="ACHOUK_004286"
     CDS             complement(2317..2670)
                     /locus_tag="ACHOUK_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9899614.1"
                     /translation="MFKTKWFAREARSHAITDEELCRAILETEQGKADALGGGVFKKR
                     LHQNRERAIILAKGVSNWFYTFLYAKQDMSNINSQELAGFREIAKHYAFLTKAQLTAM
                     INTKELTEICYDCKN"
     gene            2904..4094
                     /gene="nepI"
                     /locus_tag="ACHOUK_004287"
     CDS             2904..4094
                     /gene="nepI"
                     /locus_tag="ACHOUK_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFP9899615.1"
                     /translation="MSEFIAENCGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGCFLGELIGWRNVFNAAAAMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMTLAGFGVDGLTLVLLSFGIASFVGTSLSSFIL
                     KRSVKLALAGAPFVLALSALVLTLWGSDKIVATGVAIIWGLTFALIPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(4135..4428)
                     /gene="yicS"
                     /locus_tag="ACHOUK_004288"
     CDS             complement(4135..4428)
                     /gene="yicS"
                     /locus_tag="ACHOUK_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFP9899616.1"
                     /translation="MKPTMLLMITVFLIFPALSQAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            4650..5468
                     /gene="nlpA"
                     /locus_tag="ACHOUK_004289"
     CDS             4650..5468
                     /gene="nlpA"
                     /locus_tag="ACHOUK_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFP9899617.1"
                     /translation="MKLTTHHLRAGAALLLAGVLLAGCDQSSSDEKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNNGELDANVFQHRPFLEQDNQTHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIEQTGLSPVNDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(5472..6395)
                     /gene="yicL"
                     /locus_tag="ACHOUK_004290"
     CDS             complement(5472..6395)
                     /gene="yicL"
                     /locus_tag="ACHOUK_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFP9899618.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLIGTFLLVTHGNPTSLSISPAALF
                     WGIASAFTAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYAGQGTNFIVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(6506..6679)
                     /locus_tag="ACHOUK_004291"
                     /pseudo
     CDS             complement(6506..6679)
                     /locus_tag="ACHOUK_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            6859..6964
                     /locus_tag="ACHOUK_004292"
                     /pseudo
     CDS             6859..6964
                     /locus_tag="ACHOUK_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
     gene            6975..7040
                     /locus_tag="ACHOUK_004293"
                     /pseudo
     CDS             6975..7040
                     /locus_tag="ACHOUK_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444509.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            complement(7415..>7777)
                     /locus_tag="ACHOUK_004294"
     CDS             complement(7415..>7777)
                     /locus_tag="ACHOUK_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFP9899619.1"
                     /translation="RMLFVAKERRPSKIFIYQLSPDLLSVKQATIPEALNDIRVNDIS
                     GLAFNNESLMILSDESRKLLKFNLTEMSFIEMLDLTKGNHSLTSDLPQPEGIVTLPDE
                     SIYVASEPDILAKFVPNK"
BASE COUNT         1934 a         1695 c         1915 g         2233 t
ORIGIN      
        1 gttgtcattt gttttacaaa agcaagggat taaatctcct ccaatggaca aaaaaatgaa
       61 taatgacaat accgattacg tgagtaatga atcagggacg ctttcgcgat tatttaaact
      121 acctcagcat gggaccaccg tccgcacaga attgattgcg gggatgacca cttttttaac
      181 catggtgtac atcgtttttg tgaacccgca aatcctcggc gcggcacaaa tggacccgaa
      241 agtggtgttt gttaccacct gtttgattgc cggtatcggc agtattgcga tggggatatt
      301 tgctaactta cccgtggcgc tggctccggc aatggggctg aacgccttct ttgcgttcgt
      361 ggtcgtgggg gcgatgggta tctcctggca gaccgggatg ggcgcgatat tctggggcgc
      421 aattgggcta tttttgctca cgctatttcg tatccggtac tggatgatct ccaacattcc
      481 attaagttta cgtattggta tcaccagcgg aatcggatta tttatcgcct taatgggatt
      541 aaaaaatact ggcgttattg tcgccaataa agacactctg gtgatgattg gcgatttaag
      601 ttctcacggc gtgttgttag gtattttagg gttttttatt ataaccgtgt tgtcatcacg
      661 tcattttcat gccgcggtgc ttgtttctat tgtggtgacg tcttgctgtg gattattttt
      721 cggtgatgtt cattttagcg gcgtctattc cattccgcct gatattagcg gcgtcattgg
      781 tgaagtagat ttgagcggcg cgttaacact tgaactcgcc ggtatcattt tctcctttat
      841 gctgatcaac ctatttgatt catcaggaac attaattggt gtaactgata aagctggctt
      901 aatagatagt aacggtaaat tccccaatat gaataaggcg ctatatgttg atagcgtagt
      961 tcggtggcgg gcgcgtttat cggcacctcg tctgttaccg cctatattga aagtacttct
     1021 ggtgtggcag tcggtggtcg cacggggctg actgcggttg tggtcggcgt tatgttcctg
     1081 ttggttatgt tcttctcacc gctggtggcg atggttcctc cttacgcaac cgccggagcg
     1141 ttaatctttg ttggcgtgtt gatgacctca agtctggcgc gcgttaactg ggatgatttt
     1201 accgaatcgg tgcctgcgtt tgttaccacg gtgatgatgc cctttacttt ctcgatcacc
     1261 gaagggattg cacttggctt tatgtcgtac tgcatcatga aagtgtgcac cgggcgctgg
     1321 cgcgatctga acctgtgtgt ggtggtggtc gcagctctgt ttgcactgaa gattattctg
     1381 gtggattagt aactggcgag ggtttccaga tatcatgagt tctgattacg caggagaact
     1441 catgatctgg ataatgctcg ccacgctggc ggtagtgttt gtggttggtt ttcgggtgct
     1501 gacatccggg gccagaaaag cgattcgccg tctcagcgat cggctgaata tcgatgtcgt
     1561 acccgtggag tcgatggtcg atcaaatggg aaagtcagcc ggtgacgaat ttttacgtta
     1621 tttgcatcgt ccggatgagt cgcacctgca aaacgccgcg caggtgttgc tcatctggca
     1681 aattgtcatt gtcgatggta gcgaacagaa cctgctgcaa tggcatcgga ttttacaaaa
     1741 agcccgcctt gccgctccca ttactgatgc tcaggtcagg ctggcgctgg gttttctgcg
     1801 cgaaaccgaa cctgaaatgc aggatattaa tgcttttcag atgcgctata acgcgttctt
     1861 tcagcctgcc gagggcgttc actggctgca ttaaaaccgc ttcgtcttat cagcctacag
     1921 accgcgcctg acctgtaggg tggataaggc gttcacgccg catccggtat cggaactgac
     1981 agtaggtgaa cgcattatct acgttctatt tacagcagca cctccaaccc gtgtttttgc
     2041 acaatgctga gcagttttaa tgaaatgcca ttggggcgtt tagcgccact ttcccatttt
     2101 tgtacagttg aaacgctggt attgagatag tgagcgaaga cgggctgact cacttttaat
     2161 ttttcccgca gtgcttttat ctcaacaggt tgtaagtctt gcacgctggt caggcaggct
     2221 ttgtcaaaag tacgcatcgt ttcagcagaa atagcattga cgctggacaa tccagccgct
     2281 gcgctgtgaa tagcctcaaa tgcaggactt ttaaatttag tttttgcagt catagcagat
     2341 ctctgtcagt tctttagtgt taatcattgc tgttagttgg gctttcgtca ggaaggcata
     2401 atgctttgca atttcacgaa acccagccag ttcctgacta ttgatgttcg acatatcctg
     2461 ttttgcatat aaaaacgtgt aaaaccaatt gcttacccct ttcgccagaa taattgcacg
     2521 ctcacgattt tggtgaaggc gctttttaaa aacgccaccg ccaagtgcat cagctttacc
     2581 ttgttctgtt tctaaaatgg cccggcacaa ctcttcatct gttattgcat gagacctggc
     2641 ttcccttgca aaccatttgg ttttaaacac gcgtatatcg ttgaggtcct gttattaaag
     2701 tatatcacca agtgcaatac tttatccctt gttcccaaca caatcgcgaa aatacatatc
     2761 accttgtcct atgattttta tttcttcgag cagcctcgag cttttcccac aacaccgtaa
     2821 aagttgcaaa aacgttaaat gcgtcacaca tttcaatgtt aaagtggtcg gcttttcccc
     2881 tgaaacatgc cacgggtaac accatgagtg aatttattgc cgaaaactgc ggcgcggatg
     2941 ccatcacccg accgaactgg tcagccgttt tctcggtggc gttttgcgtc gcctgtctga
     3001 ttatcgttga gtttttgcct gtcagtttgt tgacgccaat ggcccaggat ttaggcattt
     3061 cggaaggggt tgccgggcaa tcggtgaccg tgaccgcctt tgtggcaatg tttgccagtt
     3121 tgtttattac ccagacaatt caggccactg accgccgcta cgttgttatt ttgtttgccg
     3181 ttttgctgac gctctcctgc ttgctggttt cctttgctaa ctcattcagt ttgcttttaa
     3241 tcggtcgtgc ctgtctgggg ctggcgctgg gcgggttctg ggcgatgtcg gcgtcgctga
     3301 ccatgcgtct ggtgccgccg cgtacggtgc cgaaggcgct gtcggtgatc ttcggcgcgg
     3361 tttctattgc gctggtgatt gccgcgccgt tgggctgttt tttaggcgag cttatcggtt
     3421 ggcgcaatgt ctttaatgcg gcggcggcga tgggtgtgct gtgtattttc tggattatca
     3481 aatcactgcc ttcgctgcca ggtgaacctt cgcatcagaa acaaaatact ttccgcttat
     3541 tacaacgtcc gggagtgatg gccgggatga tcgccatctt tatgtccttt gccggacagt
     3601 ttgctttttt cacttatatt cgcccagtgt atatgactct ggcgggattc ggtgttgatg
     3661 gcttaaccct ggtgctgctg agttttggta tcgccagctt tgtcgggaca tcgctttcgt
     3721 cgtttattct caaacgttcg gtgaaactgg ccttagcggg cgcaccgttt gtgctcgccc
     3781 tgagcgcgct ggtactgacg ctgtggggaa gcgataaaat tgttgctact ggtgtggcga
     3841 ttatctgggg gctgaccttt gctttaattc ccgttggttg gtcaacgtgg atcactcgct
     3901 cattggccga tcaggcagaa aaggctggat ctattcaggt tgccgttatt cagcttgcca
     3961 atacctgcgg tgcggcaatc ggcggttatg cgttggataa cattggtctg acttcgccgc
     4021 tgatgttgtc cggcacattg atgttgctga ctgcattgtt ggtgactgca aaggtgaaaa
     4081 tgaagaaatc ctgatatcag gtaaagaagc gttggtcagt ggttgttgcc gttcttacat
     4141 atccgggcat tctaccttac ccaacgcttc ccaataattg ctctgattat tacgttccat
     4201 cgccacgatg gcctcgcgta tatgttgttt attgttttca tctgacaaca ttaacttttc
     4261 ccacgcttca tctgataatg acgcctgtgg cgtgcaaatt ttacgtaaat cttgcaatac
     4321 cgtggttttc tcttttgccg actgtagaga actgaatggc gactcggcct gagaaagggc
     4381 tggaaaaatc aggaatacgg taatcatgag tagcatcgtt ggcttcatag cggcaacctt
     4441 ctgcaaatgt gcatcgaatg aagcataagt gtagtttgct tttgccgctc tcatcatgct
     4501 gtacgaaagg tgtttgcgtg gtcagtaaga agtgccagac tttatattcc acttttattc
     4561 ctttttattc ttatcgatag cgttttgttt tttaaactgc agcgacctta ccgctatagt
     4621 ccggtaatca ttaataaaag gataaaaaaa tgaaactgac aacacatcat ctacgggcgg
     4681 gggccgcatt attactggca ggagttctgc tggcaggttg cgaccagagc agcagcgatg
     4741 aaaagcatat taaagttggc gttattaatg gcgcagaaca agatgtcgca gaagtcgcta
     4801 aaaaggtagc aaaagagaaa tatggcctcg atgttgaact ggtaggattt agcggttcgt
     4861 tgctaccgaa cgatgcgaca aataatggcg agcttgatgc gaacgtcttc cagcatcgac
     4921 cctttcttga acaagataat cagacgcatg gttataaact ggtagcggtg ggaaatacgt
     4981 ttgtcttccc tatggcgggc tattccaaaa aaatcaaaac cgtggcgcaa ataaaagaag
     5041 gtgcgacagt ggcgatcccc aacgacccga ctaaccttgg tcgcgcgctt ttactgctgc
     5101 aaaaagagaa gctaattact ctgaaagagg gaaaagggtt attacctacc gcgttggata
     5161 taaccgataa tccacgccat ttgcagataa tggagctgga aggggcacag ttgccacgtg
     5221 tactggacga cccgaaagtg gatgtagcga ttatcagcac cacttacatc gagcaaacag
     5281 gactttctcc ggttaacgac agcgtattta ttgaagataa aaattcgccg tatgtgaata
     5341 ttctggtggc acgggaagat aataagaatg cagaaaacgt gaaggaattt ctgcaatctt
     5401 atcaatcacc cgaagtcgct aaagcggcag aaaccatctt taacggtggt gcggtgcctg
     5461 gctggtaatt ctcactcatg ccgcgccgga cgatttattt ttctggcgcg gcggcgggaa
     5521 tccattgaaa tcaaaatcac cgatgacaga atcagcagcg ttcccagcca gtccggcaat
     5581 gtgaaggtga tccccagcaa cagcaaagag agcagcgcgc tacttaatgg ttcagcacag
     5641 cttaaaatgc tggcttttgg accgccaatt aattgtgctc ctttcaggta cagactaaat
     5701 gtcagggacg taccaatgac caccaaataa aaaaacgcca gaatcaaact tccgttaacg
     5761 ataaagtttg tcccttgtcc ggcataaaag ggcagcagga tcagaccgcc aatgagcata
     5821 ctccagccga cgaccggtaa cgtgccatag cgggcaatca gcgttgaggg ataggtggta
     5881 tagaatgcag cagtaaatgc cgaggcaatg ccccagaata acgcggcagg agagatcgat
     5941 aatgacgtcg gattaccgtg tgtcaccaat aaaaaagtcc cgatcagcga tgtcaaaata
     6001 gcgcagaaaa ccagaatgcc cgggcgggat ttacgcacca gtgagaacca ggcgacgata
     6061 atagtcggtg agaggaattg cagcaccgtt gccgtggctg cgttcgattt ttcgatggtt
     6121 agcaaaaaag tgagctgtac ggttagcgcg ccaaccacgg aaaaaatcag taggctaatg
     6181 gcatctttgt gattgttaat aatagaaaag attttatcac catgaacaaa tgatagcgtc
     6241 agcagaatca aaccggcgaa tatcaaacgc gtcatagtca aaaactgcga cgacatctgg
     6301 ctttgctcca tgatgtattg cgcgcagacc cctgaacttc cccacaacac ggcggcaatc
     6361 agaacgttca gcatcccctt tctggtggaa cccatttttc cctctgcata ctgttttgtc
     6421 gtttatgtat gcaggcatga tagcaaaaat ggcgaggatg gctcagtttg cgccacgccg
     6481 atttttctta gcccagactg gtgagctaaa tatctttaat aaacagcaag caaatcattg
     6541 caatacccaa catccctatc gccagccaga acaacgtgtg gtagctccag atttcaacca
     6601 taatttcgtc aacagagccg gcgataatct agccgaagcg tgaggtattt gcatataacg
     6661 ttgttgcgga acctattctt atttatcgga gtgccttgcc agtaatttac tgacgaggat
     6721 cctcataata gcgttaccca tctgttgcat tgagacttta caggccgtta ttctaaatga
     6781 ccaaaattaa atcagtgcat aaggggaagt attctgtagt gatgtctccc tgagatgcag
     6841 gttccactgg agaaaattat gagcagtttg tttcagaatg aaataaagga taaatttaaa
     6901 actggacttg catatgtgcg ggggccagaa aaaagcggat cttccgctca aaatgcaggt
     6961 agcgaaggtg gtaaaaaatg cgccgggaga agcagaagat tgtcccgcga tgtccgtttg
     7021 agaaacacga gtttgattaa acgcgtgagc atgatgatat tagtagctga ccagagaaat
     7081 tatatccgtc gtaagcaata aaaatcagaa gtgatgttat ggatattttt aacctgaata
     7141 actccgtaag tacggggtta ttcaggtttt tgctagcctg tgcttttcta taccagatgt
     7201 ggtgtgaaca ctctattctg catacaaatt aaatatattg taggatctaa tataagtctg
     7261 ataatactaa tagactgagt aagattctgg ttgataataa ctgatatcct tttcaactat
     7321 taatgtgttt atttagcgtg aaattatact cgtattttta taactttatg agattgccag
     7381 gtttggtcga taaataataa ggccagtgac agacttactt attagggaca aatttcgcga
     7441 ggatatcagg ttcactggca acataaatgc tttcatcagg cagagttaca attccttcag
     7501 gttggggcaa atcactggtc agagaatgat ttccttttgt caggtcaagc atctcaatga
     7561 aagacatctc tgtcaggtta aacttgagta atttacgcga ttcatcggac agaatcatca
     7621 gactttcatt attaaaggcc aggccggata tatcgttaac acgtatatca tttagtgcct
     7681 ctggaatagt tgcttgcttt acactcagca ggtctggaga aagttgatat ataaatattt
     7741 tacttgggcg tctttctttt gcgacaaaca gcatccg
//
LOCUS       DAZKIP010000172         7707 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZKIP010000172 DAZKIP010000000
VERSION     DAZKIP010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.174"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            1..390
                     /gene="ydjO"
                     /locus_tag="ACHOUK_004295"
                     /pseudo
     CDS             1..390
                     /gene="ydjO"
                     /locus_tag="ACHOUK_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="protein YdjO"
     gene            complement(394..1785)
                     /gene="tcyP"
                     /locus_tag="ACHOUK_004296"
     CDS             complement(394..1785)
                     /gene="tcyP"
                     /locus_tag="ACHOUK_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFP9899620.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHH"
     gene            complement(1918..2508)
                     /gene="ydjM"
                     /locus_tag="ACHOUK_004297"
     CDS             complement(1918..2508)
                     /gene="ydjM"
                     /locus_tag="ACHOUK_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP9899621.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2671..3339)
                     /gene="hxpB"
                     /locus_tag="ACHOUK_004298"
     CDS             complement(2671..3339)
                     /gene="hxpB"
                     /locus_tag="ACHOUK_004298"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFP9899622.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3486..4022
                     /gene="yniB"
                     /locus_tag="ACHOUK_004299"
     CDS             3486..4022
                     /gene="yniB"
                     /locus_tag="ACHOUK_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFP9899623.1"
                     /translation="MTYQQAGRIAVLKRIMGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGTRMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4063..4923)
                     /gene="yniA"
                     /locus_tag="ACHOUK_004300"
     CDS             complement(4063..4923)
                     /gene="yniA"
                     /locus_tag="ACHOUK_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFP9899624.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(5029..5319)
                     /gene="ghoS"
                     /locus_tag="ACHOUK_004301"
     CDS             complement(5029..5319)
                     /gene="ghoS"
                     /locus_tag="ACHOUK_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFP9899625.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5420..6349)
                     /gene="pfkB"
                     /locus_tag="ACHOUK_004302"
     CDS             complement(5420..6349)
                     /gene="pfkB"
                     /locus_tag="ACHOUK_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFP9899626.1"
                     /translation="MVRIYTLTLTPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            6636..7394
                     /gene="ydiY"
                     /locus_tag="ACHOUK_004303"
     CDS             6636..7394
                     /gene="ydiY"
                     /locus_tag="ACHOUK_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFP9899627.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(7447..7554)
                     /gene="yniD"
                     /locus_tag="ACHOUK_004304"
     CDS             complement(7447..7554)
                     /gene="yniD"
                     /locus_tag="ACHOUK_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFP9899628.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
BASE COUNT         1997 a         1881 c         1867 g         1962 t
ORIGIN      
        1 aaggactata tactatgaac aatattatat acatcctgtc gggagcattt ttgctgcgat
       61 tcaaaaaaat gagagtctcc taaagtttcc ttcgataata tcaatgctta atatcaatct
      121 tctttttaat ccactaaatc ttccgggaat gggaagtggt atacttgaag atattatgag
      181 tattccagat agttcactta gaaaacgtct cgggtatgag gttttaagct tctcgctaca
      241 ggcacattca ttgagccagg aatgtattga taagttggac attttttcgc tgacgatctg
      301 tttaaatatg agtcagtatg catcgccgcg atggaacatt taaaaagtaa ggcaacggcc
      361 cctttacaaa acggaccgtt gccagcataa gaattaatgg tgtgccagtt cggcgtcgtc
      421 ttcgctatcc agaatggctt tatcggtttg cttcagccac tggctggtca gcgtgccagc
      481 tgtcatcgag ccactaacgt ttagcgccgt acggcccatg tcgataagcg gttcaacgga
      541 gattaacagc gccaccaggg ttactggcag gcccatcgca ggcagcacaa tcagtgcggc
      601 gaaagttgca ccaccaccga caccggcaac gcctgcggag ctaacggtaa caataccgac
      661 cagcgtcgca atccacatcg ggtccagcgg gttaatgcca accgtaggcg caaccatcac
      721 cgccagcatt gccggataca aaccggcgca gccgttctga ccaatggtcg caccgaaaga
      781 ggcggcgaaa ctggcgatgg attcaggaac accgagtcga cgcgtttgtg cttccacatt
      841 cagtgggata gacgcagcac tggaacggct ggtaaaggca aacgtcagca caggccatac
      901 cttacggaag tacttcagcg gactcacgcc attaatgccc agcagaatgc catgcactgc
      961 aaacataatc agcagaccga ggtaggacgc gacaacgaaa cttcccagtt tgatgatgtc
     1021 ttgcaggtta gaacctgcaa ccactttggt cattagtgcc agaacgccgt aaggggtcaa
     1081 ctgcatgacc aggcgaacta gtttcatcac ccagctttgc agggtatcga tagcggttaa
     1141 gacgcgttca cctttcggcg catcatcctt cagcagtttc agcgcagcta caccgaggaa
     1201 tgcggcaaaa attaccacgc tgataattga cgtcggattg gctccggtca gatcggcaaa
     1261 cgggtttttc gggataaagg acaagaccag ctgcggaacg ctcaggtcag agactttacc
     1321 aacatagtta ctttcgatgg cgttcagacg tgcagtttct gcaccaccct gaaccagacc
     1381 ttcagccgtc aaaccaaaca ggttggttac cagcacaccg accagcgccg caatcagcgt
     1441 ggtaaacaaa agcgtaccaa tggtcagaaa actgattttg cctaactgag atgcgttatg
     1501 cagacgggca accgcgctca gaatagaggc gaagactaac ggcataacga tcatttgcag
     1561 cagttgaaca tagccgttac caacaatgtt aaaccactgt acagaatctt taagtacctg
     1621 gctgtcagaa ccataaatgg tatgcagggc aaggccaaaa accacaccca tcaccagacc
     1681 caccagcact tttttcgcca gactccactg tttatggcgg gtctgagcca gcgcaaacag
     1741 cagtacaacg aacaccacga tgttcgcaat taatggaaag ttcatccccg ttctcctgat
     1801 cttattattt ctatcactgc gtagcagtca tagtgttggc gcaagattaa cagaactgtg
     1861 agctggctct tatattcaaa tggaattgct tattacagaa aggcgatttt tgccttttta
     1921 gtattcaacc tgatgcttaa taagccgatg aaactggatc tgcaaggtat tgatcatttg
     1981 cgatgaccaa cgaacagcgc tgttttcggg taatgaatga ggcatccata ccgaccagac
     2041 aaataatgcc atgcagataa aacgttccag ttggttaccc ttttggggaa ccaggatagg
     2101 caagcggaaa cgccagcggc atggccagag caggggaaca ccggcgggtg tcagcatatc
     2161 ggcaagtatg tggcttaaat aacccagcac cattccttgt agcgcatcag ccggaatgaa
     2221 ccagctttca ggaaccttaa ggtaaaaggt tgccagcagc gcaaataccg ccagcagact
     2281 gtgggtaaaa ccacgatgcc caaaagcgcg ggcgatcggt tttgatatcc actttaatcg
     2341 ctgcccaaga aacgactttg ggtgatcgat gtccggtaac aaacacgtca ggattgcgga
     2401 agggacaata tgccaccagt caccctgtgc cagcacgggc gtcagctcgg catttttggc
     2461 aaataccgca caagcaatag aaaagagaag gtgaccttcc gccgtcatga taaaactcac
     2521 taaactgtcg atacgtacag tataggattt ttatacagtg cacggaagtg gtgacggtgt
     2581 aactctttct cttaaattga cgattaatcc gtcagttgaa tgcaaattca gataaagaac
     2641 caggggcagc gtgatcactg cccctggtca ttaaccgaga aggtcttttg cggtgagttc
     2701 tgtcagcgat gagagtttga cgtttgctaa tacaaaacgt ggatcatttt gcgcttctgg
     2761 cgcaggaacg acgatggaac gcatgcgggc tgctttagag gcgatcatgc catttaccga
     2821 atcctccagc gctacgcagg tcagtgggtc aacgcccagt tttgctgcgc agtcgagata
     2881 tacttgcgga tgcggcttgc tgtaaggcag tttttcggcc gaggcgaggg catcgaaact
     2941 gtcgcgtaag tcaaacatgg tcaacacttt ttccagcata tgtagtggtg acgcggaggc
     3001 cagtcccacc aataaacctt gttctttgca taacgcaacg gcttcgcgca cgcctggtaa
     3061 taatggacgt gtctcttcaa ccagtgaaat ggcacgggca ataacccgtt ctactacttc
     3121 ctgacggctt ggcccattcc acggttgccg ggcgtaccaa agatcgacca ccatatcgat
     3181 gcgtaaacct aaggtgtccg gcagctcgtt acgacgggag atatccaccc ccaggcttgc
     3241 catcacatcc agttcggctc gatcccataa aggttctgag tcgataagta atccatccat
     3301 atcaaaaatt gcagcaagaa tctgacgcgg ggttgacatt acaacctctc ctttagagca
     3361 ttatgtgtaa ggtattaagc cgtgcttatg attaagcgcc tatcatatcg cctttaaaga
     3421 atgggcgaaa acggatatct acaggtaaac taagtgaaac gacgcgtctt aatcaaggag
     3481 aactcatgac gtatcaacaa gctggacgca ttgctgtttt gaaaaggatt atgggttggg
     3541 tgatttttat acctgcattg atctctacat tgatttcttt gctgaaattt atgaataccc
     3601 ggcaggaaaa ccaggaaggc attaatgcgg tcatgctcga ctttactcat gtcatgatcg
     3661 atatgatgca ggcgaatacg ccttttttga atctgttctg gtataactcc ccgacgccca
     3721 attttaatgg tggcgtgaac gttatgttct gggtgatttt catcctgatt tttgttggac
     3781 tggcgttgca ggattccggt acccggatga gtcgccaggc gcgcttcttg cgggaaggtg
     3841 ttgaagatca actcattctg gaaaaagcca aaggggaaga ggggctaacg cgtgaacaaa
     3901 ttgagtcacg tattgttgtt ccgcaccata ctattttcct gcagtttttc tcactgtata
     3961 tcctgccggt tatttgtatt gctgcaggtt atgtgttctt ttctctgctt gggtttattt
     4021 aatcaaaagg ccgcacggag cggccattat cttcaaccca tatcacgcag ctaataatct
     4081 atccagtgac tgctgagcaa taaccaaatg ctgaccgcca aataatcttg cacgatttaa
     4141 cagcgtgtag agttggtaaa ccggttgacg ttcaaggaaa tcggcaggta gcggggatac
     4201 tgactgatag ccgtcataga tttgtggcgg ctgttcagta tgcagcggta acatcgccag
     4261 gtcgcactct cggtcacccc agtagcaggc cgggtcgaaa atgtacgggc catccggacc
     4321 cagcgcacag ttgccggacc ataaatcgcc gtgcaacaga gaaggctgcg gttgatgtga
     4381 ggcgagacgc tgctgaatat gctcgacgat ggcatcgata ttgccgaaag cgatcccttt
     4441 ttctgctgcc agttccaact gccagccaat ccgttgttca gcaaaaaacg ttgaccagcg
     4501 acgttgccag gtgttgggct gtggagttgt ggagagcgag ttatcaaaat cgagtccaaa
     4561 ttgtggttgg tcgctccatt gatgtaaacg cgcaatttgc tgaccaagaa taaatgcgct
     4621 atgcgcatcc agcggacgag gtgggagata atccatcacc agaaaactgt agtcacggtc
     4681 agcgccaact gcccaaacct taggcacggt gacggtttta ctacgcgaca gtaactccag
     4741 ttggtcggct tcggcggtaa aaccgggaag cagttccctt tcatcacatt tgacgaaaaa
     4801 gtcatgtcct gcatagcgca aatgccatgc ggcatggact tctccgccag gcagttcatt
     4861 acgcagttcg atttcgcctt cacctaactg ctcgctcaaa agacgactga ttgcctgcca
     4921 catgatgtct ctcccatgtt gtctgccaga tcataaggtt agcgcaagaa tgcggtgtaa
     4981 aaacacgagc taacgcacac cacagactga ttattgccca aaaaccattt attttctgtt
     5041 gctattccat tcctcccagg tcgtgatggt gatttcagga tctttgccgg ttgcactttg
     5101 ggtaagcccc gaaaccagtt ctctaatttc ttcttcactt tgggtactga taagtccaaa
     5161 agtgttagtc cccagctcat ggatatttcc ctcatcatcc gtcatggtga gcaaaaaacc
     5221 gtcgcgagtc aggtaattat tcaactcgtt aatttcagtc aatgtatcct catgcaacat
     5281 gacggttatg acataacgga caagatcgcc actggccata gttcacccct ttgttatcac
     5341 atgacttttg agcatagtcg gagaaacgcg ttgccgacag gttggtgatg attcccccaa
     5401 tgctggggga atgtttttgt tagcgggaaa ggtaagcgta aattttttgc gtatcgtcat
     5461 gggagcacag acgtgttccc tgattgagtg tggctgcact ccccgcagct acgccaaaac
     5521 gaaccatctc ttcaagagag gcattttctg ccagtttcag tgtcatcgcg ccgaccatgc
     5581 tatcaccagc gccaacggta ctctggcttt tcaccggtgg tggcaccacc tgaatgcagt
     5641 tttcactatc aacacccagc gctccttgtg gacccaggga aacgacaacc cgtttggcct
     5701 tgccgctatt aacgatttcc tgcgcggctt tgcggacatc gtctggctgg gtgagttcgc
     5761 gattcaccag cgcactgagt tctttttggt taggcttaac caactcgatg ttaccaatcg
     5821 ccagtgctgc acttaacgct tcgccagaac tgtcgatgat gcagcggatc ccttggtttt
     5881 gcgcagcgga aatcagttgg gttaattttt ccagcttcac acctggcggc aggcttccgc
     5941 ttatgaccag gatggccccg gattcaattt ccagaacttg ctcttcaagc tggcgaaact
     6001 catcttcatt taatgccgcg cctggcataa caaaacgata ctgctcaccg cttgcttcca
     6061 catgtacgtg taaattctgc cgggtccagt ctttggcttc tacagtagcg acggggacat
     6121 tttcatccgc caacagtgaa accaggtgtt cgccggtcgc gccacccgcc gggaagatcg
     6181 ctgtggcact gcctccaaga tgggcaatgg cgcgggcgac gttgatgccg ccgcccccgg
     6241 gttcgaacac cggtgcggta cagcgcagtt ttccttcggg ataaatttgc ggggtaattg
     6301 ttgcgctatc gagagagggc gtaagtgtca acgtatagat acgtaccatc atttcctcct
     6361 ataggctgat ttcagtctgg caccgaatca gcggaaagtg aagaaattaa caatatgatt
     6421 tattggagct ttatttaaaa ttttgcagat aaatatatat aaataaaaat ctgttttcgc
     6481 cacatgctta ttcaaaaaat gaaataagaa tacattgata agttattcgc gcctttttat
     6541 aattcgttac ctttccaggg acgctaatct ttgatcctta agaaagtttc cgggaccgcg
     6601 atggtctctt tttttgttgt acggactctt gataaatgaa gcttttgaag acagttcctg
     6661 ccatcgttat gttggcgggg ggcatgtttg cgtcactgaa tgcagctgcc gatgattccg
     6721 tttttactgt catggacgat cctgccagcg ctaaaaaacc tttcgaaggt aatctgaacg
     6781 cagggtatct tgcacaatca ggcaatacga aaagctcctc acttacggct gacaccacca
     6841 tgacctggta tggacaaacc actgcctggt cgctgtgggg gaacgccagt aatacttctt
     6901 ctaatgatga gcgttcttca gaaaaatatg cggcgggcgg gcgtagccgt tttaatttaa
     6961 ctgattatga ctatttattt ggtcaggcaa gctggctgac agaccgttat aacggctatc
     7021 gagagcgcga tgtgttgacc gcgggttatg gtcgtcagtt tttgaatggc ccggtacaca
     7081 gtttccgctt tgaattcggt ccaggtgtgc gttacgacaa atataccgac aatgccagtg
     7141 aaactcagcc gctgggctat gcctccggtg cttatgcatg gcagcttact gacaacgcta
     7201 aatttacgca gggtgtttcc gtctttggcg cggaagacac aacgctgaac tccgaaagcg
     7261 cattaaatgt tgctattaat gagcattttg gactgaaggt ggcttacaac gtcacatgga
     7321 actctgaacc tccagagtct gcaccggaac ataccgaccg tcggacaacc ttatcgctgg
     7381 gctacagcat gtaaaattat ctgccggacc gtgttatgcg gtctggctgt cttcgtttgt
     7441 cactgctcag ccccaaatca ttgctgccca acgtagcaac aacatagcgc cacagattgc
     7501 cagtagcacc accaccatct tccagtgttt tttatcaaag cgtttagtcg gcataccagt
     7561 gtcctgcata tctcttgtgc gtgataacgg agtgtaacaa aagggacagg catcaaggga
     7621 ttttttgtta ttacagccgt cgggtggcgt tgtacacctg atcactgttt ctgcgtttta
     7681 tgaaaaaata aaattatgta tcgatat
//
LOCUS       DAZKIP010000173         7644 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZKIP010000173 DAZKIP010000000
VERSION     DAZKIP010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.175"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            36..398
                     /gene="rusA"
                     /locus_tag="ACHOUK_004305"
     CDS             36..398
                     /gene="rusA"
                     /locus_tag="ACHOUK_004305"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFP9899629.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVARIIKG
                     SMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGKLELTITELGNE"
     gene            395..535
                     /locus_tag="ACHOUK_004306"
     CDS             395..535
                     /locus_tag="ACHOUK_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFP9899630.1"
                     /translation="MMFESYMAERLRHRWMRLRLYRFPGSVLTDYRILKNYAKTLKGA
                     AA"
     gene            532..1221
                     /locus_tag="ACHOUK_004307"
     CDS             532..1221
                     /locus_tag="ACHOUK_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFP9899631.1"
                     /translation="MNTQYLQYVREQLIVATADLSGATKGQLEAWLEHAQFDTGTYKR
                     KKPRILDEVTGRMITLDNPPISGKQSYAKGSSIALVSQVEFSTSSWRRAVLSLEEHQK
                     AWLLWSYSESVRWEHQVTITQWAWSEFKTLLGTRKIAGKTLERLKKLIWLAAQDVKNE
                     LAGRKTYEYQELASLVGVTSKNWSETFTERWVAMKHIFLQLDSQALLLLTKTRSKQKT
                     TFSQQDIAKLD"
     gene            1543..1848
                     /locus_tag="ACHOUK_004308"
     CDS             1543..1848
                     /locus_tag="ACHOUK_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFP9899632.1"
                     /translation="MHEKESLAGAFWLVLLIIAGWGGLVRYLIDVKQSKATWSWINAL
                     AQIVVSGFTGVIGGLISIESGFSIYMILATAGISGAMGSVALTYFWERLTGVKNAKS"
     gene            1835..2311
                     /locus_tag="ACHOUK_004309"
     CDS             1835..2311
                     /locus_tag="ACHOUK_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFP9899633.1"
                     /translation="MQNLNPQRKAFLDMLAWSEGTDNGRQPTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLHPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVRESEV"
     gene            2528..2710
                     /gene="rzoD"
                     /locus_tag="ACHOUK_004310"
     CDS             2528..2710
                     /gene="rzoD"
                     /locus_tag="ACHOUK_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HFP9899634.1"
                     /translation="MRKLKMMLCMMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(2801..3094)
                     /gene="bor"
                     /locus_tag="ACHOUK_004311"
     CDS             complement(2801..3094)
                     /gene="bor"
                     /locus_tag="ACHOUK_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serum resistance lipoprotein Bor"
                     /protein_id="HFP9899635.1"
                     /translation="MKKMLLATALALLITGCAQQTFTVQNKPAAVTPKETITHHFFVS
                     GIGQKKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            complement(3386..3796)
                     /gene="ybcV"
                     /locus_tag="ACHOUK_004312"
     CDS             complement(3386..3796)
                     /gene="ybcV"
                     /locus_tag="ACHOUK_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFP9899636.1"
                     /translation="MAQVAIFKEIFDQVRKDLNCELFYSELKRHNVSHYIYYLATDNI
                     HIVLENDNTVLIKGLKKVVNVKFSRNTHLIETSYDRLKSREITFQQYRENLAKAGVFR
                     WVTNIHEHKRYYYAFDNSLLFTESIQNTTQIFPR"
     gene            4082..4288
                     /gene="ybcW"
                     /locus_tag="ACHOUK_004313"
     CDS             4082..4288
                     /gene="ybcW"
                     /locus_tag="ACHOUK_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbcW"
                     /protein_id="HFP9899637.1"
                     /translation="MNKEQSADDPSVDLIRVKNMLNSTISMSYPDVVIACIEHKVSLE
                     AFRAIEAALVKHDNNMKDYSLVVD"
     gene            complement(4453..4647)
                     /gene="ylcI"
                     /locus_tag="ACHOUK_004314"
     CDS             complement(4453..4647)
                     /gene="ylcI"
                     /locus_tag="ACHOUK_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFP9899638.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALDQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIKSDEE"
     gene            5036..5581
                     /gene="nohD"
                     /locus_tag="ACHOUK_004315"
     CDS             5036..5581
                     /gene="nohD"
                     /locus_tag="ACHOUK_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFP9899639.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AAIKWYAERDAEIENEKLRREVEELRQDSETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            5556..7481
                     /locus_tag="ACHOUK_004316"
     CDS             5556..7481
                     /locus_tag="ACHOUK_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFP9899640.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADASYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSASVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLAEYARALSGEDE"
     gene            7478..>7644
                     /locus_tag="ACHOUK_004317"
     CDS             7478..>7644
                     /locus_tag="ACHOUK_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFP9899641.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTTTSVSDLK
                     KYIAELEVQTG"
BASE COUNT         1988 a         1701 c         2000 g         1955 t
ORIGIN      
        1 caggttatct ggctgaaaga gggggttatt aaggcgtgaa tacctacagc atcacattac
       61 cctggcctcc gagcaataat cgctactacc ggcataatcg cgggcgcacg cacatcagcg
      121 cagaagggca ggcataccgt gataacgtcg cccgaatcat caaaggctcc atgctggata
      181 tcggcctggc tatgccagtg aaaatccgta ttgagtgcca catgccggat cgccgtcgcc
      241 gtgacctgga taatctgcaa aaagccgctt ttgacgcact caccaaagca ggtttctggc
      301 tggatgatgc tcaggtcgtt gattaccgtg ttgtgaagat gcccgttacc aaaggtggga
      361 agctggagct gaccatcacc gaactgggga atgaatgatg tttgagtctt atatggcaga
      421 acgtcttcgc caccgctgga tgcgcctgcg cttatatcgt tttcctggtt ctgttttgac
      481 cgattaccgg atactgaaga attacgccaa aacactgaaa ggagctgccg catgaatacc
      541 caatatttac agtatgtccg cgagcaactc attgtggcta ccgctgattt gagcggagca
      601 acgaaaggac agcttgaagc ctggctggag catgcacaat ttgatactgg tacatacaaa
      661 cgaaagaagc cgcgcattct ggatgaggta actggcagga tgattacgct ggataatccg
      721 ccgatttccg gtaagcagtc gtacgcaaaa ggttcatcca ttgcactggt cagccaggtt
      781 gagttctcaa cctcgtcatg gcgccgcgcg gttctgtctc tcgaagaaca tcagaaagcg
      841 tggttgctgt ggagttacag cgaaagtgtt cgctgggaac atcaggtcac cataacgcag
      901 tgggcatgga gcgagtttaa gactctgttg ggtaccagga aaattgcagg taagacactg
      961 gaacgtttga agaagttgat ctggctggcg gcacaggatg tgaagaacga gctggcaggg
     1021 cgtaagacct atgaatacca ggagctggca tcactggtgg gagtgacatc aaaaaactgg
     1081 tctgagacat ttactgaacg ctgggttgca atgaaacaca tttttctaca gcttgatagc
     1141 caagctttat tgcttttaac gaaaacacgt tcaaaacaaa agaccacatt ttcacagcaa
     1201 gatattgcaa aactggatta aaaatcatat attttatgta aatctgatat tttgccaatg
     1261 ttgtacgcac tggcagtaat ccaaattcaa gcccgaggtt taaagccttg ggcttttctg
     1321 tttctgaacg gtgagtagcc ttccaaccta ccccagccag ggggtcttca gctgttgagt
     1381 tgatattgct tagccctctg ttgccagcta catgctggct tttttattcc aggcttgtgg
     1441 ggagcatcaa ctccgtgctt tgtcgttaaa ttaccccgtg agcctgattt ctgacattta
     1501 acgtcccggc cttttgtcgg cggcgaaaca ttggctattc atatgcacga aaaagagagc
     1561 cttgccggag cgttctggct cgttttgctg atcatcgcag gttggggcgg tctggtccgc
     1621 tacctgatag atgtgaagca gagtaaagca acgtggagtt ggataaatgc tctggctcaa
     1681 atagtggtat caggattcac cggtgttatt ggtggcctga tcagcatcga aagtggattc
     1741 agtatttaca tgattctcgc gacagcgggg attagtggtg cgatgggttc ggttgcactg
     1801 acgtacttct gggaacgact gacaggggtg aaaaatgcaa aatcttaatc ctcagcgtaa
     1861 agccttcctc gatatgttgg cgtggtcaga aggaacggat aacgggcgac aaccgacacg
     1921 taaccacggt tatgatgtta ttgttggcgg cgaactgttt actgattact ccgatcaccc
     1981 tcgcaaactt gtcacgctac accccaaact caaatcaaca gccgccggac gttatcagct
     2041 tctttcacgc tggtgggatg cttaccgtaa acagcttggt ttgaaagact tctcccccaa
     2101 aagccaggac gcagtggcat tgcagcagat taaagagcgt ggtgctttac ctatgattga
     2161 tcgcggtgat atccgtcagg caatcgaccg ttgcagtaat atctgggctt cactgccggg
     2221 cgctggttat ggtcagttcg agcataaggc tgacagcctg attgcaaaat tcaaagaagc
     2281 tggcggaacg gtcagagaga gtgaggtatg agcagaataa ccgcgattat ctccgctctg
     2341 ctcatctgca tcatcgtctg cctgtcatgg gctgttaatc attaccgtga taacgccatt
     2401 acctacaaag cccagcgcga caaaaatgcc agagaactga agctggcgaa cgtggcaatt
     2461 actgacatgc agatgcgtca gcgtgatgtt gctgcgctcg atgcaaaata cacgaaggag
     2521 ttagctgatg cgaaagctga aaatgatgct ctgcatgatg atgttgccgc tggtcgtcgt
     2581 cggttgcaca tcaaagcagt ctgtcagtca gtgcgtgaag ccaccaccgc ctccggcgtg
     2641 gataatgcag cctccccccg actggcagac accgctgaac gggattattt caccctcaga
     2701 gagaggctga tcactatgca aaaacaactg gaaggaaccc agaagtatat taatgagcag
     2761 tgcagataga gctgcccata tcgatgggca gctcatgcaa ttattgtgag caatacacac
     2821 gcgcttccag cggagtataa atgcctaaag taataaaacc gagcaatcca tttacgaatg
     2881 tttgctgggt ttctgtttta acaacatttt ctgcgccgcc acaaatttta gctgcatcga
     2941 cagttttctt ctgcccaatt ccagagacga agaaatggtg ggtgatggtt tcctttggtg
     3001 ttactgctgc cggtttgttt tgaacagtaa acgtctgttg ggcacatcct gtaataagca
     3061 gggccagcgc agtagcgagt agcatttttt tcatggtgtt attcccgatg ctttttaaag
     3121 ttcgcagaat cgtatgtgta gaaaattaaa caaaccctaa acaatgagtt gaaatctcat
     3181 attgttaata tttattaatg tatgtcagat gcgaaggatc gtcactgtat tcccggatta
     3241 actatgtccg cagccctgac agggaaactc ctctgcggga gtgtccggga ataattaata
     3301 acgatgcaca cagggtttag cgcgtacacg tattgcatta tgccaacgcc ccggtgctga
     3361 cacggaagaa accggacgtg atgatttagc gtggaaagat ttgtgtagtg ttctgaatgc
     3421 tctcagtaaa tagtaatgaa ttatcaaagg catagtaata tcttttatgt tcgtggatat
     3481 ttgtaaccca tcggaaaact cctgctttag caagattttc cctgtattgc tgaaatgtga
     3541 tttctcttga tttcaaccta tcataggacg tttctataag atgcgtattt cttgagaatt
     3601 taacattcac aaccttttta agtcctttta ttaacacagt gttatcgttt tctaacacaa
     3661 tgtgaatatt atctgtggct agatagtaaa tataatgtga gacattgtga cgttttagtt
     3721 cagaataaaa caattcacag tttaaatctt ttcgcacttg atcgaatatt tctttaaaaa
     3781 tggcaacctg agccattggt aaaaccttcc atgtgatacg agggcgcgta gtttgcatta
     3841 tcgtttttat cgtttcaatc tggtctgacc tctttgtgtt ttgttgatga tttatgtcaa
     3901 atattaggaa tgttttcaat taatagtatt ggttgcgtaa caaagtgcgg tcctgctggc
     3961 attctggagg gaaatacaac cgacagatgt atgtaaggcc aacgtgctca aatcttcata
     4021 cagaaagatt tgaagtaata ttttaaccgc tagatgaaga gcaagcgcat ggagcgacaa
     4081 aatgaataaa gaacaatctg ctgatgatcc ctccgtggat ctgattcgtg taaaaaatat
     4141 gcttaatagc accatttcta tgagttaccc tgatgttgta attgcatgta tagaacataa
     4201 ggtgtctctg gaagcattca gggcaattga ggcagcgttg gtgaagcacg ataataatat
     4261 gaaggattat tccctggtgg ttgactgatc accataactg ctaatcattc aaactactta
     4321 acctgtgaca gagccaacac gcagtctgtc actgtcagga aagtggtaaa actgcaactc
     4381 aattactgca atgccctcgt aattaagtga atttacaata tcgtcctgtt cggagggaag
     4441 aacgcgggat gttcattctt catcactttt aattgatgta tatgctctct tttctgacgt
     4501 tagcctccga cggcaggctt caatgaccca ggctgagaaa tttccggacc ctttttgatc
     4561 aagagcgatg ttaatttgtt caatcatttg gttaggaaag cggatgttgc gggttgttgt
     4621 tctgcgggtt ctgttcttcg ttgacatgag gttgccccgt attcagtgtc gctgatttgt
     4681 attgtctgaa gttgttttta cgttaagttg atgcggatca attaatacga tacctgcgtc
     4741 ataattgatt atttgacgtg gtttgatggc gtagatgcac gttgtgacat gtagatgata
     4801 attattatca ttttgcgggt cctttccggc gatccgacag gttacggggc ggcgacctcg
     4861 cggtttttca ctatttatga aaattttccg gtttaaggcg tttccgttct tcttcgccgt
     4921 aacctaatgt ttttatttaa aacaccccct gaaaagaaag gaaacgacag gtgctgaaaa
     4981 cgggcttttt ggcctctgtc gtttcctttc tctgtttttg tccgtggaat gaacaatgga
     5041 agtcaacaaa aagcagctgg ctgacatttt cggcgcgagt atccgtacca ttcagaactg
     5101 gcaggagcag ggaatgcccg ttctgcgggg tggtggcaag ggtaatgagg tactttatga
     5161 ctctgccgcc gccataaaat ggtatgccga aagggatgct gaaattgaga acgaaaagct
     5221 gcgccgggaa gttgaagaac tgaggcagga cagcgagaca gatctccagc cagggactat
     5281 tgagtacgaa cgccatcgac ttacgcgtgc gcaggccgac gcacaggaac tgaagaatgc
     5341 cagagactca gctgaagtgg tggaaaccgc attctgtact ttcgtgctgt cgcggatcgc
     5401 aggtgaaatt gccagtattc tcgacgggat ccccctgtcg gtgcagcggc gttttccgga
     5461 actggaaaac cgacatgttg atttcctgaa acgggatatc atcaaagcca tgaacaaagc
     5521 agccgcgctg gatgaactga taccggggtt gctgagtgaa tatatcgaac agtcaggtta
     5581 acaggctgcg gcattttgtc cgcgccgggc ttcgctcact gttcaggccg gagccacaga
     5641 ccgccgttga atgggcggat gccagttact atctcccaaa agaatccgca taccaggaag
     5701 ggcgctggga aacactgccc tttcagcggg ccatcatgaa tgcgatgggc agtgactaca
     5761 tccgcgaggt gaatgtggtg aagtctgccc gtgttggtta ttccaaaatg ctgctgggtg
     5821 tttatgccta cttcatagag cataagcagc gcaacaccct tatctggttg ccgacggatg
     5881 gtgatgccga gaactttatg aaaacccacg ttgagccgac tattcgtgat attccgtcgc
     5941 tgctggcgct ggccccgtgg tatggcaaaa agcaccggga taacacgctc accatgaagc
     6001 gtttcaccaa tgggcgtggc ttctggtgcc tgggcggtaa agcggcaaaa aactaccgtg
     6061 aaaagtcagt ggatgtggcg ggttatgatg aacttgctgc ctttgatgag gatattgaac
     6121 aggaaggctc tccgacgttc ctgggcgata agcgtattga aggctcggtc tggccaaagt
     6181 ccatccgtgg ctccacgccc aaagtgagag gcacctgcca gattgagcgt gcagccagtg
     6241 aatccccgca ttttatgcgt tttcatgttg cctgcccgca ctgcggggag gagcagtacc
     6301 ttaaatttgg cgataaagag acgccgtttg gcctcaaatg gacgccggat gatccctcca
     6361 gcgtgtttta tctctgcgag cataatgcct gcgtcatccg tcagcaggag ctggacttta
     6421 ctgatgcccg ttatatctgc gaaaagaccg ggatctggac ccgtgatggc attctctggt
     6481 tttcgtcatc cggtgaagag attgaaccgc ctgacagtgt gacctttcac atctggacgg
     6541 cgtacagccc gttcaccacc tgggttcaga ttgtcaaaga ctggatgaag acgaaagggg
     6601 atacgggaaa acgtaaaacc ttcgtgaaca ccacgctcgg tgagacatgg gaagcgaaga
     6661 tcggcgaacg tccggatgct gaagtgatgg cagagcggaa agagcattat tcagcgtccg
     6721 ttcctgaccg tgtggcttac ctgaccgccg gtatcgactc ccagctggat cgctacgaaa
     6781 tgcgcgtatg gggatggggg ccgggtgagg aaagctggct gattgatcgg cagattatta
     6841 tgggccgcca cgacgatgaa cagacgctgc tgcgtgtgga tgaggccatc aataaaacct
     6901 atacccgccg gaatggtgca gaaatgtcgg tatcccgtat ctgctgggat actggcggga
     6961 ttgacccgac tattgtgtat gaacgctcga aaaagcatgg gctgttccgg gtgatcccca
     7021 ttaaaggggc atccgtctac ggaaagccgg tggccagcat gccacgtaag cgaaacaaaa
     7081 acggggttta ccttaccgaa atcggtacgg ataccgcgaa agagcagatt tataaccgct
     7141 tcacactgac gccggaaggg gatgaaccgc ttcccggtgc cgttcacttc ccgaataacc
     7201 cggatatttt tgatctgacc gaagcgcagc aactgactgc tgaagagcag gtcgaaaaat
     7261 gggtggatgg caggaaaaaa atactgtggg acagcaaaaa gcgacgcaat gaggcgctcg
     7321 actgcttcgt ttatgcgctg gcggcgctgc gcatcagtat ttcccgctgg cagctggatc
     7381 tcagtgcact gctggcgagc ctgcaggaag aggatggtgc agcaaccaac aagaaaacac
     7441 tggcagaata cgcccgtgcc ttatccggag aggatgaatg acgcgacagg aagaacttgc
     7501 cgctgcccgt gcggcactgc atgacctgat gacaggaaaa cgggtggcaa cggtacagaa
     7561 agacggacgg cgagtggagt ttacgaccac ttccgtgtct gacctgaaaa aatacattgc
     7621 tgagctggaa gtgcagaccg gcat
//
LOCUS       DAZKIP010000174         7629 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZKIP010000174 DAZKIP010000000
VERSION     DAZKIP010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7629
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.176"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..4848)
                     /locus_tag="ACHOUK_004318"
     CDS             complement(<1..4848)
                     /locus_tag="ACHOUK_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP9899642.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMLSQRAMAASQMKRAANDKAIAQM
                     LASKKSGPPAARLGDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLVP
                     ALMYASYKASDYVEEKQNQLESWINSFCDTDGAINTGSKNVKINGKPAARAAVTLPPP
                     PPPGAIPEVPQGEPSWGDIATDLLESAAEKAVPLAKAWGNAVITLTDSNAGFMDRVSA
                     GASLLFPAGPVLMEFATMVGGRGEIKKDVDFPEAGEDTALCDKENKPPRIAQGSSNVF
                     INNQPAARKGDKLECSAAIVEGSPDVFIGGEQVTYLDIQPEFPPWQRMILGGITIASY
                     LLPPAGLLGKLGNLAKLGKLGNLLGKSGKLLGAKLGALLGKTGNSLKSIANKVIRWVT
                     DPVDPVTGAYCDERTDFTLGQTLPLSFTRFHSSVLPLHGLTGVGWSDSWSEYAWVREQ
                     GNRVDVISLGATLNFAFDGESDTAVNPYHAQYILRRRDDYLELFDRDALSSRFFYDAF
                     PGMRLRHPVTDDTSDDRLAHSPADRMYMLGGMSDTASNRITFERDTQYRITGVSHTDG
                     IRLKLTYHASGYLKAIHRTDNGIQTLATYEQDARLDYHLFYEYDAADRIIRWSDNDQT
                     WSRFTYDAQGRCVTVTGAEGYYNATLDYGDGCTTVTDGKGIHRYYYDPDGNILREEAP
                     DGSTTTYEWDEFHHLLARHSPAGRVEKFEYNAAHGQLSRYTAADGADWQYCYDERGLL
                     SNITAPAGQTWTQQCDERGLPVSLVSPQGEETRLAYTPQGLLSGIFRQDERRLGIEYD
                     HHNWPETLTDVMGREHHTEYSGHDLPVKMRGPGGQSVRLQWQQHHKLSGLERAGTGAE
                     GFRYDRHGNLLAYTDGNGVVWTMEYGPFDLPVARTDGEGHRWQYRYDKDTLQLTEVIN
                     PQGESYLYILDNCGRVTEERDWGGVVCRYRYDADGLCTARVNGLEETILYSRDAAGRL
                     AEVITPEGKTQYAYDKSGRLTGIFSPDGTSQRTGYDERGRVNVTTQGRRAIEYHYPDE
                     HTVIRCILPPEDERDRHPDGSLLKTTYRYNAAGELTEVILPGDETLTFSRDEAGREVL
                     RHSNRGFACEQGWNAAGQPVSQRAGLFPAEATWGGLLPSLLREYRYDSAGNVSGVTSR
                     EDYGRETHREYRLDRNGQVTAVTASGTGLGYGEGDETYGYDSCGYLKAQSAGRHRISG
                     ETDQYAAGHRLKQAGNTQYDYDAAGRMVSRTKHRDGYRPETERFRWDSRDQLTGYRSA
                     QGEQWEYRHDASGRRTEKRCDRKKIRFTYLWDGDSIAEIREYRDDKLYSVRHLVFNGF
                     ELISQQFSRVRQPHPSVAPQWVTRTNHAVSDLTGRPLMLFNSEGKTVWRPGQTSLWGL
                     ALSLPADTDYPDPRGERDPEADPGLLYAGQWQDAESGLCYNRFRYYEPETGMYLVSDP
                     LGLQGGEQTYRYVPNPCGYIDPLGLAICQLARWTKWGSEQSNISDVLNSLGNRALKYA
                     NGDWIKSEAAFNKYINMINKRLELTGSKFRVEIQPAIKNGERVPATTNGPFKVNGKWT
                     SGTHYTGGSKRLDAGIIDITSPTNQYGLHPVIEGFDITLNKTKPSAVDIYSDVFGGID
                     INDFRL"
     gene            complement(4868..5329)
                     /locus_tag="ACHOUK_004319"
     CDS             complement(4868..5329)
                     /locus_tag="ACHOUK_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFP9899643.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREPVSQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(5357..7258)
                     /locus_tag="ACHOUK_004320"
     CDS             complement(5357..7258)
                     /locus_tag="ACHOUK_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFP9899644.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MEGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     RGAGTALENHFAVIPADRTWRPQPRLKPLVDGPQSAVVTGPEGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENHTPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGNGIQAYTAIKEIMLAVEESKIALTPDGIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
BASE COUNT         1479 a         2278 c         2012 g         1860 t
ORIGIN      
        1 taaccgaaag tcgttaatat caatcccacc aaacacatct gaatatatat ccactgctga
       61 tggttttgtt ttattaagtg ttatatcaaa tccttcaata actggatgta atccatattg
      121 gtttgtagga gatgtgatat caataatacc ggcatctaga cgtttggaac cacctgtata
      181 atgagtgccg gatgtccact taccatttac tttaaatggt ccattcgttg tcgcaggaac
      241 tcgctctcca ttttttatgg ctggttgaat ctcaactcta aatttactac ctgttaattc
      301 tagtctttta tttatcatgt ttatgtattt attgaatgca gcctctgatt ttatccaatc
      361 accattagca tatttaagtg ctctattccc taatgagttc aaaacatcag atatgttgct
      421 ttgctcactc ccccatttcg tccagcgggc taactgacat atagccagcc ccaaaggatc
      481 gatataccca caaggattcg gcacataccg ataagtctgc tcccctccct gcaaccccag
      541 cggatcactc accaggtaca tcccggtttc cggctcgtag taccggaacc ggttatagca
      601 cagccccgat tctgcatcct gccactgtcc cgcatacagc aggccggggt ccgcttccgg
      661 gtcccgctcc ccgcgcgggt ccgggtagtc tgtgtctgcg ggcaggctga gtgccagccc
      721 ccacaggctg gtctgccccg gccgccagac ggttttacct tcactgttaa agagcatcag
      781 cgggcggccc gtcaggtcgc tcaccgcatg attcgttcgc gtcacccact gcggggccac
      841 ggacggatgc ggctgtcgta cccggctgaa ctgctggctt atcagctcaa agccgttaaa
      901 caccaggtgc cttacgctgt acagtttatc atcgcggtat tcccggattt ccgcaatact
      961 gtcgccgtcc cacaggtacg taaaacggat tttcttccgg tcgcagcgtt tttccgtccg
     1021 tctgccgctg gcgtcgtggc ggtattccca ctgctccccc tgtgcgctgc gatacccggt
     1081 cagctggtcc cggctgtccc accggaaccg ctctgtttct gggcggtagc cgtcacggtg
     1141 ttttgtgcgg ctgaccatcc ggcctgcggc gtcatagtca tactgtgtgt ttccggcctg
     1201 tttcagccgg tggcctgcgg catactggtc agtctctccg cttatccggt gtctgcccgc
     1261 agactgcgcc ttcaggtagc cgcagctgtc atagccataa gtctcgtcgc cttcgccata
     1321 gcccagcccg gtgcctgagg ctgtcaccgc cgtgacctgg ccgttccggt caagccggta
     1381 ctcccggtgt gtttcccgtc cgtaatcttc ccggctggtg acgcctgata cgttacccgc
     1441 gctgtcgtaa cggtattccc gtagcagtga agggagcagt ccgccccatg tggcttccgc
     1501 cgggaaaagt ccggcgcgct ggctgacagg ctgaccggct gcattccagc cctgttcaca
     1561 ggcaaaaccc cggttactgt gccggagcac ttcacgtccc gcctcatcac ggctgaacgt
     1621 cagcgtctca tcccccggca ggataacctc cgtcagttct ccggcggcgt tgtagcggta
     1681 tgtggttttc agcagggatc cgtcggggtg tctgtcgcgt tcatcttccg gtggcaggat
     1741 acagcggatg acggtgtgtt cgtcggggta gtggtattca atggcccgtc ggccctgagt
     1801 ggtgacattc acccgcccgc gttcatcata gccggtgcgc tgtgatgtgc cgtccgggct
     1861 gaagataccc gtcagcctgc cggatttgtc atacgcatac tgcgttttgc cttccggagt
     1921 gatgacttct gccaggcggc ctgcggcatc ccggctgtag aggatggttt cctccaggcc
     1981 gttgaccctg gcggtacaca ggccatcagc gtcataacgg taacgacaga ccacgccgcc
     2041 ccagtcacgt tcttccgtca cccggccaca gttgtccaga atataaagat aagactcgcc
     2101 ctgcgggtta atgacttctg tcagttgcag cgtgtcttta tcgtagcggt actgccagcg
     2161 gtggccttca ccgtccgttc gcgccaccgg caaatcaaac gggccgtact ccattgtcca
     2221 gacaacgccg ttaccgtccg tgtacgccag caggttgccg tggcggtcgt agcggaatcc
     2281 ttccgcgccg gttcctgccc gctcaaggcc actcagttta tggtgctgct gccactgcaa
     2341 ccgcactgac tgaccgccgg ggccgcgcat cttcaccggc aggtcgtgac cgctgtattc
     2401 ggtgtggtgt tcgcggccca tcacgtcggt gagtgtttcc ggccagttgt ggtggtcgta
     2461 ctctatgccc agacgccgtt catcctggcg gaatatcccc gacagcagcc cctgaggggt
     2521 gtacgccagc cgggtctctt cgccctgtgg cgataccaga ctcaccggca ggccgcgttc
     2581 atcacactgc tgcgtccacg tctgcccggc aggggcggtg atgttgctga gcaggccgcg
     2641 ctcatcatag cagtactgcc aatccgcgcc gtctgccgcc gtgtaacggc ttaactgacc
     2701 gtgtgcggcg ttgtattcaa acttctccac ccgcccggca ggggagtggc gggccagcag
     2761 gtgatggaat tcatcccatt catacgtggt ggtgctgccg tccggcgctt cttcccgcag
     2821 aatattgccg tcaggatcat agtaataacg gtgaatgccc ttgccgtccg tcacggtggt
     2881 gcagccgtca ccatagtcca gcgtggcgtt gtaatagccc tccgccccgg tgacggtcac
     2941 gcaccggccc tgtgcatcgt aggtgaaacg gctccacgtc tggtcgttat cggaccagcg
     3001 gatgatccgg tccgcagcgt cgtactcata aaacaggtgg tagtccagcc gcgcatcctg
     3061 ttcgtaggtc gccagcgtct gtatgccgtt atccgtgcgg tgaatggctt tcaggtagcc
     3121 gctggcgtgg tacgtcagtt taagccggat cccgtcggtg tgactgacac ccgtgatccg
     3181 gtactgggtg tcgcgctcaa acgtgatgcg gttgctggcg gtgtcgctca tcccgcccag
     3241 catgtacatc cggtctgcgg ggctgtgtgc caggcggtca tcgctggtat cgtcagtcac
     3301 cgggtggcgc agacgcattc ccggaaaggc gtcataaaag aagcggctgc tcagtgcatc
     3361 cctgtcgaac agctccagat aatcatcacg gcggcgcaga atgtactggg cgtgatacgg
     3421 gttaaccgcc gtatcacttt caccgtcgaa ggcgaagttc agcgtggctc ccaggctgat
     3481 gacatccacc cggtttccct gttcacgcac ccaggcgtat tcgctccagg agtcgctcca
     3541 gcccacgccc gtcaggccat gcagcggcag tacagaactg tggaaacggg tgaaggagag
     3601 ggggagtgtc tggcccagcg taaagtcggt acgttcgtcg cagtatgcgc cggttaccgg
     3661 atcgacagga tctgttaccc atctgatgac tttattggca atacttttta acgaattacc
     3721 tgttttcccc agcaacgcgc cgagctttgc gcccagcagc ttaccgcttt tccccagcag
     3781 gtttcccagt ttgcccagtt tcgccagatt ccccagtttt cccagcagtc ctgctggcgg
     3841 cagaagatag ctggctatcg ttattcctcc caggatcatt ctctgccatg gcgggaactc
     3901 cggctggata tccagatagg tgacctgctc acccccaata aagacgtccg gcgaaccttc
     3961 cacgattgcc gcgctgcact ccagtttgtc gcccttgcgc gcggcaggct gattgttgat
     4021 aaagacgttg ctgctgccct gggctatcct cggtggtttg ttctccttgt cgcagagcgc
     4081 cgtgtcctca ccggcttccg ggaaatccac atcttttttg atttcgccac gcccgcccac
     4141 catggtggca aactccatta ataccggacc ggcgggaaac agaagcgatg cgccggcgct
     4201 gacgcgatcc ataaaaccgg cattgctgtc cgtcagggtg ataacagcgt tcccccaggc
     4261 cttcgccagt ggtactgctt tttccgctgc cgattcaagc aggtcagtgg caatatcacc
     4321 ccatgagggt tccccctgtg ggacttcagg tattgctcca ggtgggggag gagggggaag
     4381 ggtgacggct gcacgggctg ctggctttcc gttaattttt acatttttag aaccggtatt
     4441 gatggcgccg tccgtgtcac aaaagctgtt tatccatgat tcaagctggt tctgtttctc
     4501 ctccacataa tcactcgcct tatagctggc atacatcagc gcaggcacca gaaccagcgc
     4561 ataagggccg gtggcacagg cggccataat caggcaacct tctgctatgg tcactatcgc
     4621 ccccagcacg gcccctgcca gcgcccccaa aaaactcttg tgctgaattt catcgcccag
     4681 cctggcggcg ggggggccag acttttttga tgccaacatc tgtgcaatgg ctttgtcgtt
     4741 ggctgcccgt ttcatctggc tggcggccat cgcccgctgc gacagcatcc gcattgccag
     4801 cgtaccgcct gcggtcgctc cctgtggccc gcctggtcct tcactcatgt tttatccctc
     4861 gtttatgtca gcgcggctgg tccttttgga tgtcattctc cggtggcaac ggggtgaagc
     4921 tctttatcac ctccagcagc atcgtctttt gactgttctt cagggataag gaggtgctga
     4981 aagtgaaggt cagcagcgta tctccacgcc gctggaatac ggctatctgg ctaatccgcc
     5041 cctgattccc ttcatcttcc ggaccgttcc aggaataatc cagcagccag gccggggcct
     5101 ctgccagagt gatttcccgg caggcatgtt cggtgtaccc atcaagtttg ccctggtaca
     5161 gcgccagcgt ttcctggtac agctctggtg tcagggttcc ctgtgaaacg ggttcgcggg
     5221 taatgaccag gttgatgcca ctgttatccg tggataccag gacctgcatc gactgatcct
     5281 gccattgcga tggcagttga atatgacctt cattaaaagt gaactgcata aaatactccc
     5341 tgttaatctg agtgaatcag ttgatgaaaa cagaaccgcc cacgatggtg atgccatcct
     5401 tcgacagcct gattaccgtt gattccccga cctgtagctg aatgccatcc ggcgtcagcg
     5461 caattttgct ttcctccacg gccagcataa tttccttaat tgccgtataa gcctgtatac
     5521 cgttgccttc gccaccattg tttacagcgt caacatgcac cgaaccggca atcgtgcctc
     5581 tgtcctgaaa gacatcctca gataaagacc ctttcagtac tccggtgatc ttgtggcctt
     5641 tttccaccgt aagaaggcgg ttaccttcct ggatcgtgac cgtctggtca ttttttacgg
     5701 tttcgcgatg atcgtgtttt acggtggtgg tacggtcgtt gagcacttcg gtatccatgt
     5761 tcttctgcgc gtggatgtag acctgttctc tccctgtcgc atcatcaaac ttcagctcat
     5821 taaacccgct gcccatatac gttttggaac ggatggtcat ctgcgtcttc gtccccggca
     5881 ggctgccggg ggtgtggttt tcctggtggt aggtgcgccc cataatgatc ggctggtccg
     5941 gatcgccgtt gaggaagtcc actatcacct cctgacccac acgcggtatc gccaggttgc
     6001 caaaaccggt gcctgcccac gcctgtgcca cacggatcca gcacgagctg tcctggtctg
     6061 ccgggttata acggtcccag ttgaatttca cccgcactcg gccatgttca tcacagaaga
     6121 tttcctcacc ctccggcccc gtcacgacgg cgctctgcgg accgtccacc agcggtttta
     6181 gccgcggctg gggtcgccat gtccggtctg ccgggatcac cgcaaaatgg ttctccagcg
     6241 ccgtccctgc tccccgcctt cccggcaccg cctgcggctg ctcgccgtgc agttcacttg
     6301 ccaccacctg ccattcccgg ttcaggttcg cctgcggatg ccccgtcagc acaattcgcc
     6361 gtcccggcca tatctccggc gagcgactca ttccccgcgc ggtttctgca ttgtttcgcc
     6421 agccctccat ctgccagcgg gcaaagttct gcccgtgggc gcccttgaaa cgtccggggt
     6481 agtcatacac ctcatactgc gtgcgctggt aatcctggtg ctggccttcc tgatcaaaac
     6541 gtcctgccca gccggggcgt ttaaaggtgt agtctttggt caccacggaa gaagggcgga
     6601 tttgtgcgct gtagcgaaac tggctgatgc agagggtgct cacctcggta cgggtgttcg
     6661 ggttccaggg gatttcaaaa gattcgggca gatggcggac cgtgtcgcac agcaccaggc
     6721 tctggtcggt acttttgtaa gcatgctcct cataaaagaa gatgccttcc tccgccgcca
     6781 tccggcacag gaaatcgtaa tcagtctcac cgtactggac acaaaactca cgggaaggat
     6841 gcggttcact gaacagcgga ctccactcgg tcaccccgtt ttcctgcaat atcgtgccga
     6901 ggatgctttt gatgtcctcg ttctggaaga tacggaagtt ctggcgcaga ccggcacgcc
     6961 acagcggcgg gtgcaccttc atgctgtaca gcatctggtt tttgtcgttc tcccccagtt
     7021 caaaccaggt caccacacct ttcacccggc gctgtacatc atcaccctgc catattgtca
     7081 ggtaggccat tttgtccagc acctgcgcaa attcaaggga gagaaactgc tggctgacca
     7141 aggagagatc gagggaaaag agcgaagaga gtgactgggt gagatgaaag gaaaccaccg
     7201 caaaagcgtc cggtggcagg ccgtccactt ccagtgtgaa acgtaatccg gttgacatag
     7261 caacctccgg taaaatgaat aataatccgc accgtacaac tgcggataat gagataagcg
     7321 ggacgactta agacgttatt agggaaggaa caaaaaatgc tatgattatg tgaggatttt
     7381 gtttaaatat tactttacag aaacgggtgt ctgttcagtc aaaaaaataa tcacaagctc
     7441 aacttattgt ttcctgacgt tattcaggac aggtgcgcac atcacagcga gtaacaaaaa
     7501 cgcaaagtta aaccaatatt cataatatgt ttttattcac aactaaataa cccaattaac
     7561 atcattaaaa tgaatgattg cttaaaaaat atatgctacc gctccaccct gataacatga
     7621 tctgcacat
//
LOCUS       DAZKIP010000175         7601 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZKIP010000175 DAZKIP010000000
VERSION     DAZKIP010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.177"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..918
                     /locus_tag="ACHOUK_004321"
     CDS             <1..918
                     /locus_tag="ACHOUK_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFP9899645.1"
                     /translation="GKELLEKVDLTEDNASRLDEFSKEWKDANDKWNAMWGVKIEQTK
                     DGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETPMFVAQGNQIFMNDVF
                     LKRLTAPTITSGGNPPAFSLTPDGKLTAKNADISGSVNANSGTLNNVTINENCQIKGK
                     LSANQIEGDIVKTVSKSFPRTSTYASGTITVRISDDQKFDRQVMIPPVLFRGGKHENF
                     NSNNQQSYWYSTCRLRVTRNGQEIFNQSTTDAQGVFSSVIDMPAGQGTLTLTFTVSSS
                     GANNWTPTTSISDLLVVVMKKSTAGISIS"
     gene            985..1584
                     /locus_tag="ACHOUK_004322"
     CDS             985..1584
                     /locus_tag="ACHOUK_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP9899646.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHARTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGMAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            1649..4564
                     /locus_tag="ACHOUK_004323"
     CDS             1649..4564
                     /locus_tag="ACHOUK_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFP9899647.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMTEDDVRPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHATDAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSAGAAKTSETNAAVSQQSA
                     ATSASTATTKASEAASSARDASASKEAAKSSETSAASSASSAASSATAAGNSAKAAKT
                     SETNAKSSETAAEQSASAAAGSKTAAALSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQASAAASSASAAKTSETNAKASETSAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKSSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYELANGKYTAQDATTA
                     QKGIVQLSNATNSTSEMLAATPKSVKAAYDLANGKYTAQDATTAQKGIVQLSSATNSA
                     SETLAATPKAVKAANDNANGRVPSARKVNGKALSSDITLTPKDIGTLNSTTMSFSGGA
                     GWFKLATVTMPQASSVVSITLIGGAGFNVGSPQQAGISELVLRAGNGNPKGITGALWQ
                     RTSTGFTNFAWVNTSGDTYDIYVAIGNYATGVNIQWDYTSNASVTIHTSPAYSANKPE
                     GLTDGTVYSLYTPSEQFYPPGAPIPWPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSG
                     VIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTN
                     NTGAHTHSVSGTAASAGNHTHSVTGASAVSQWSQNGSVHKVVSAASVNTSAAGAHTHS
                     VSGTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            4564..5145
                     /locus_tag="ACHOUK_004324"
     CDS             4564..5145
                     /locus_tag="ACHOUK_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP9899648.1"
                     /translation="MAFRMSEQPRTIKIYNLLTGTNEFIGEGDAYIPPHTGLPANSTY
                     IAPPDIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEEE
                     TSLLEAWKKYRVLLNRVDTSTAPDIEWPVIPEV"
     gene            complement(5265..6155)
                     /locus_tag="ACHOUK_004325"
     CDS             complement(5265..6155)
                     /locus_tag="ACHOUK_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFP9899649.1"
                     /translation="MFRHVKKLQYTVRVSEPNPGLANLLLEQFGGPQGELAAACRYFT
                     QGLSDEDAGRRDMLMDIATEELSHLEIIGSLVGMLNKGAKGALAEGTENEAELYRSLT
                     QNGNDSHITSLLYGGGPALTNSAGVPWTAAYVDTIGEVTADLRSNIAAEARAKIIYER
                     LINVTDDPGVKDTLAFLMTREAAHMLSFEKALHSIRNTFPPGKLPPLEKYKNVYYNMS
                     EGEDVRGSWNSDENFDYVSDPVSAVDGGDGKASINLSTKQEAMIKAMATRLKSHEDIN
                     PVTGAELAEGEPQTKINSKN"
     gene            complement(6174..6680)
                     /locus_tag="ACHOUK_004326"
     CDS             complement(6174..6680)
                     /locus_tag="ACHOUK_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFP9899650.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAEKMLESMASRIENYPELRSRIEQ
                     HISETKNQLSQLESILDRNNISRSVIKDSMSKMAAFGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASVPIIEAILNEEKQMAEWLLNHIPDTTEQFMVRSE
                     IDGVEAKK"
     gene            complement(6717..7217)
                     /locus_tag="ACHOUK_004327"
     CDS             complement(6717..7217)
                     /locus_tag="ACHOUK_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFP9899651.1"
                     /translation="MQIKSVEDIFIHLLSDTYSAEKQLTKALSKLSRSAYSDKLTAAF
                     QSHLDETHGQIERIDQVVDSEDGLKLKRIKCAAMEGLIEEANEVIESTDKNEVRDAAL
                     IAAAQKVEHYEIASYGTLVTLAEQLGYKKAAKLLKETLEEEKATDVKLTDLAFNNVNK
                     KAQDNS"
     gene            complement(7296..7478)
                     /locus_tag="ACHOUK_004328"
     CDS             complement(7296..7478)
                     /locus_tag="ACHOUK_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFP9899652.1"
                     /translation="MTEHRGGAGNFAEDRQKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQNSHGGGRKSDS"
BASE COUNT         2027 a         1803 c         1909 g         1862 t
ORIGIN      
        1 ggtaaagagc tactggaaaa agttgacctg acggaggata acgccagcag actggatgag
       61 ttttcgaaag agtggaagga tgctaacgat aagtggaatg ccatgtgggg cgtcaaaatt
      121 gagcagacca aagacggcaa acattatgtc gcgggtattg gcctcagcat ggaggacacg
      181 gaggaaggca agctgagcca gtttctggtt gccgccaatc gtatcgcgtt tattgacccg
      241 gcaaacggga atgaaacgcc gatgtttgtg gcgcagggca accagatatt catgaacgac
      301 gtgttcctga agcgcctgac ggcccccacc attaccagcg gtggaaatcc accggcattt
      361 tccctgacac cggacggaaa gctgactgct aaaaatgcgg atatcagtgg cagtgtgaat
      421 gcgaactccg ggacgctcaa caacgtcacg attaatgaga actgtcagat taaggggaaa
      481 ctgtcagcca atcagattga aggcgatatt gtcaaaacgg tcagcaagtc tttcccccgc
      541 acgagcactt atgccagtgg caccatcacg gtaagaatca gtgatgatca gaaatttgac
      601 cggcaggtca tgataccgcc agtgttattc cgcggtggta agcatgagaa tttcaacagt
      661 aataaccaac agtcatactg gtattcaacc tgccggttaa gagtgacccg caatggccag
      721 gagattttta atcagtccac gacggatgct cagggcgtat tttcctcagt tatagatatg
      781 cctgccggac aggggacgct gacactgaca ttcaccgtat cttcatcagg agcgaataac
      841 tggacaccaa caaccagtat cagcgatctg ctggttgtgg tgatgaaaaa atccacagca
      901 ggtatcagta tcagctgaat tttataaccc agaacgggcg tcagaaatga cgcctttttt
      961 attgcagaaa agcgagaggt aattatgcgt aaactttatg ccgccatttt gtccgcagcc
     1021 atttgtctgg ccgtatccgg tgcgcctgca tgggcatctg aacatcagtc cacgctgagc
     1081 gcggggtatc ttcatgcccg gaccaacgtt cccggcagtg atgatctgaa cgggattaac
     1141 gtgaaatacc gttatgagtt tacggatacg ctggggctgg tgacgtcatt cagctatgca
     1201 ggagacaaga atcgccagct gacccgttac agcgataccc gctggcatga agattccgtg
     1261 cgtaaccgct ggttcagcgt gatggcgggg ccgtctgtgc gcgtgaatga atggttcagc
     1321 gcgtatgcga tggcgggtat ggcttacagc cgtgtttcga ctttttccgg ggattacctt
     1381 cgcgtaactg acaacaaggg gaaaacgcac gatgtgctga ccggaagtga tgacggtcgc
     1441 cacagcaaca cgtctctggc gtggggggct ggcgtgcagt ttaacccgac cgaatccgtg
     1501 gccattgata ttgcttatga aggttctggc agtggtgact ggcgcactga cgggttcatc
     1561 gtgggtgttg gttataaatt ctgattagcc aggtaacaca gtgttatgac agcccgccgg
     1621 ttcaggcggg cttttttgtg gggtgaatat ggcagtaaag atttcaggtg tactgaaaga
     1681 cggcacagga aaaccggtac agaactgcac aatccagctg aaagcaaaac gtaacagtac
     1741 cacggtggtg gtgaacacgc tggcctcaga aaatccggat gaagccgggc gttacagcat
     1801 ggacgttgag tacggtcagt acagcgttat tctgttggtg gaagggttcc cgccgtcaca
     1861 tgccgggacc atcaccgtgt atgaagattc tcaacccggt acgctgaatg attttcttgg
     1921 tgccatgact gaggatgatg tccgtccgga ggcactgcgc cgttttgagc tgatggtgga
     1981 agaggtggcg cgtaacgcgt ccgcggtggc acagaacacg gcagccgcga agaagtcagc
     2041 cagcgatgcc agcacatcag cccgtgaggc ggcaacccat gcgactgatg ctgcggactc
     2101 agcacgcgca gccagcacgt cagccggaca ggccgcgtcg tcggctcagt cagcgtcttc
     2161 cagcgcagga acggcatcaa caaaggctac tgaagcatca aaaagtgctg ccgctgcaga
     2221 gtcctcaaaa agcgcggcgg ctaccagtgc cggtgcggcg aaaacgtcag aaacgaatgc
     2281 ggcagtgtca caacaatcag ccgccacttc tgcatccacc gcgaccacga aagcgtcaga
     2341 agctgcctcc tcagccaggg atgcgtcggc ttcaaaagag gcggcaaaat catcagaaac
     2401 gagcgcagcc tcgagcgcca gtagtgcagc ctcctcggca acggcggcag gcaattccgc
     2461 gaaggcggcc aaaacgtctg agacaaacgc taagtcctct gaaacggcag cagaacagag
     2521 tgcctccgca gcagcaggct caaaaacagc ggctgcatta tctgccagtg ccgcgtcaac
     2581 aagtgccggg caggcctcag ccagtgccac cgccgccgga aaatcggcag aaagtgccgc
     2641 atcgtctgct tcaacagcca caacgaaggc tggcgaagcc actgaacagg ccagcgcagc
     2701 agcgagttct gcttccgcag cgaagacatc cgaaacgaac gcgaaagcgt cggaaaccag
     2761 cgcagaatcc tcaaaaacgg ctgccgcatc gtcagccagt tcggcggcgt catcggcatc
     2821 atctgcgtct gcttcaaaag atgaggcgac cagacaagcg tcagcagcga agagcagcgc
     2881 cacgacggca tccacgaagg cgacagaggc agctggtagt gcgacggcag cagctcagag
     2941 caaaagtacg gcggaatctg cagcaacgcg cgctgagaca gcggcaaaac gggcagagga
     3001 tattgcatcc gccgtggcgc ttgaggatgc gagcacgacg aaaaagggga tagtacagct
     3061 cagcagtgcg actaacagca cttccgagtc actggcggca acgccaaaag ccgttaaggc
     3121 cgcgtatgag ctggctaacg ggaaatacac cgcacaggat gcaacgacag cacagaaagg
     3181 gatagttcag cttagcaacg cgaccaacag cacatctgaa atgctggcgg caacgccaaa
     3241 gtcggtaaag gcagcctatg accttgctaa cgggaaatat actgctcagg acgctacgac
     3301 agcacaaaaa ggaattgtcc agctcagtag tgcaaccaac agcgcatctg aaacgcttgc
     3361 cgcgacaccg aaagcagtga aagcagctaa tgataatgcg aatggtcggg taccttctgc
     3421 ccgtaaggtg aatggtaagg cgctttcatc ggatataaca ctgacgccga aagatattgg
     3481 tacgcttaac tcaacaacaa tgtcattcag cggtggtgct ggttggttca aattagcaac
     3541 ggtaaccatg ccacaggcga gttctgttgt ttcaattacg ttgattggtg gcgcgggatt
     3601 taacgtgggg tcacctcaac aggcaggtat atctgaactt gttttgcgtg caggtaatgg
     3661 taatccgaag gggattactg gtgctttatg gcagcgcaca tcgacagggt ttacaaattt
     3721 tgcctgggtc aatacatctg gtgatactta cgatatttac gttgcaatcg gaaattatgc
     3781 gactggtgta aatattcaat gggattatac cagtaatgcc agcgtgacga ttcatacgtc
     3841 accagcatat tctgctaata agccggaagg gttaacggac ggtacagttt attcactcta
     3901 tacgccatca gagcagtttt atccgcctgg cgcaccaatc ccgtggccat cagataccgt
     3961 tccgtctggc tatgccctga tgcaggggca gacttttgac aaatctgcat acccgaaact
     4021 tgcagccgct tatccgtcag gcgtgatccc tgatatgcgt ggctggacga ttaagggcaa
     4081 acctgccagt ggtcgggccg tattgtctca ggaacaggac ggcattaaat cgcacaccca
     4141 cagcgccagc gcatccagta cggatttggg gacgaaaacc acatcgtcgt ttgattacgg
     4201 cactaaatcc acgaataaca ccggggcgca cacgcacagt gtgagcggta cagccgcaag
     4261 tgccggaaac catactcata gtgtcacagg cgcatcagca gtcagccagt ggtcacaaaa
     4321 tgggtcagta cataaggtag tgtctgcggc cagtgtgaat acaagtgctg caggagcgca
     4381 cactcatagt gtcagcggca cagctgcatc tgcaggtgct cacgcacata ctgtcggtat
     4441 tggtgctcat acgcactctg ttgcgattgg ctcacatgga cacaccatca ccgttaacgc
     4501 tgcgggtaac gcggaaaaca ctgtcaaaaa catcgcattt aactacattg tgaggcttgc
     4561 ataatggcat tcagaatgag tgaacaacca cggaccataa aaatttataa tctgctgacc
     4621 ggaactaatg aatttattgg tgaaggtgac gcatatattc cgcctcatac aggtctgcca
     4681 gcaaacagta cctatattgc accgccagat attccggctg gcttcgtggc tgttttcaac
     4741 agtgatgagt catcgtggca tctcgttgaa gatcatcggg gtaaaacggt ttatgacgtg
     4801 gcttccggcg acgcgttatt tatttctgaa ctcggctcat taccggaaaa tgttacctgg
     4861 ttatcgccgg aaggggagtt tcagaagtgg aacggcacag cctgggtgaa agatgcagaa
     4921 gcagaaaaac tgttccggat ccgggaggcg gaagaaacaa aaaacagcct gatgcaggta
     4981 gccagtgagc atattgcgcc acttcaggat gctgtagatc tggaaatcgc aacggaggaa
     5041 gaaacctcat tgctggaagc ctggaaaaag tatcgggtgt tgctgaaccg tgttgataca
     5101 tcaactgcac ctgatattga gtggcctgta atacctgaag tttaaaataa aagcccgctt
     5161 aaaatatcgc gggcttcgaa atataaatgt attttcagag gctagagctt aggatatctc
     5221 agcggcaatt aacttatgag ggatagctaa ataccagaag ataattagtt tttactattt
     5281 atctttgttt gtggttctcc ttcagcaagc tcagcgccag tgacaggatt gatgtcttca
     5341 tgggatttca accttgttgc catagctttt atcatagctt cttgcttggt cgaaagattt
     5401 atcgaggcct tcccatcccc accgtcaacc gctgaaactg gatcggaaac atagtcaaag
     5461 ttttcatcac tgttccaact acctctgacg tcttcacctt ctgacatgtt gtaatatacg
     5521 tttttgtatt tctcaagtgg tggtaatttt cctggtggaa aggtgttacg aattgaatgt
     5581 aatgcttttt caaatgaaag catgtgtgct gcttcccggg tcattaaaaa tgccagagtg
     5641 tcttttactc caggatcatc agtaacatta atgagacgtt cgtaaatgat ctttgcccga
     5701 gcttcagctg caatatttga acgaagatcg gccgtgactt cgccaatagt atcaacataa
     5761 gctgcagtcc agggtactcc agctgaatta gttaatgctg ggcctcctcc gtagaggaga
     5821 gaagttatat ggctatcatt gccattttga gttaaggaac ggtaaagctc agcttcattc
     5881 tcagttcctt cagcaagtgc tccttttgca cctttgttga gcataccaac aagagaacca
     5941 ataatttcga gatgacttag ttcttctgtt gctatgtcca tcagcatatc ccttcggcct
     6001 gcatcttcat cacttaagcc ttgagtgaag tatcggcatg ctgctgcaag ctcaccctgt
     6061 ggcccaccga attgttctaa gagtagatta gccaagcctg ggtttggctc acttacacgt
     6121 actgtatatt gaagtttttt cacgtgtcta aacataatgc ctcctcaaaa gaattatttt
     6181 ttggcttcaa ctccatcaat ttcagatctg accatgaatt gctcagtggt gtcaggaata
     6241 tggttaagaa gccattcagc catttgtttt tcttcattaa gaattgcttc aataatgggt
     6301 actgaagctg tatcacccgc atttttggct gctgcgagaa gagatgtgta acacgcaatt
     6361 tcaaattgtt cgaacacata gccactgatc gaacctttga caatttcatc agatggaaaa
     6421 attccaccaa tggattgtcc aaacgcggcc attttactca tagagtcttt aatgacagag
     6481 cgcgaaatat tattacgatc aagtatgctt tcaagctgtg aaagctggtt ctttgtttcg
     6541 ctaatgtgct gctcaattct ggaacgtaac tcaggatagt tttctatccg gctggccata
     6601 gactcaagca ttttttcagc ttgtttttcc atcgcatgtg catcacgaag ccagtcatgg
     6661 taatgttcaa ttctattcat aatctgttta tcctaaaaga aattgaagtc tatagttcag
     6721 gagttatcct gagctttttt attaacgtta ttaaatgcca ggtctgttaa tttgacatca
     6781 gtagcttttt cctcttccag tgtttctttc aggagtttag ccgctttttt atatcctaat
     6841 tgctcagcga gtgttaccaa agtgccataa ctggcaattt catagtgctc tactttttgt
     6901 gctgcagcaa tcaatgcagc atcacgtact tcatttttat cagtgctttc aattacctca
     6961 ttcgcctctt cgataagtcc ttccattgcg gcacacttaa ttcttttcaa tttaagacca
     7021 tcttcagaat caacaacctg atcgatgcgt tcaatttgac catgtgtctc atcaagatgt
     7081 gactgaaaag cagcggttaa tttatcacta tatgcagagc gactaagttt agacagtgcc
     7141 tttgttagtt gtttctcagc gctgtatgta tccgataata agtggatgaa gatatcttca
     7201 acagatttaa tttgcataat cactccagag ttttcactgc gagcccgtag gctcgcagtt
     7261 agcttcagtt attcacgaat gtagggggtg ataaattaag agtcagattt acggccgcca
     7321 ccatggctat tctgaccacc ttttttccct gcttctgatg ctctttgtgg atcgttttta
     7381 aaattaccgc cactatgttg gccgccttta cgaccagcgt cagaagcttt ttgtcgatct
     7441 tcagcgaaat tacctgcacc gccacgatgt tcggtcatat catttctcct ctgattttca
     7501 aattgtaaat aacatgtgcc ttgtcagcac ccaattaact ctagtaacaa ataagaaagt
     7561 gtcaaataag agattggtat gcgcatctta atgtgagaaa t
//
LOCUS       DAZKIP010000176         7530 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZKIP010000176 DAZKIP010000000
VERSION     DAZKIP010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.178"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(48..235)
                     /locus_tag="ACHOUK_004329"
                     /pseudo
     CDS             complement(48..235)
                     /locus_tag="ACHOUK_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611456.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(355..1137)
                     /locus_tag="ACHOUK_004330"
                     /pseudo
     CDS             complement(355..1137)
                     /locus_tag="ACHOUK_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008815333.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1853..2311)
                     /locus_tag="ACHOUK_004331"
     CDS             complement(1853..2311)
                     /locus_tag="ACHOUK_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9899653.1"
                     /translation="MTLRKLFGQRVKELRIATGMSQEAFADRCGFARSYMSRIERGGS
                     NASLDAIEVLANALSVEPWRLLVSDSSEDSDPELLVPYAADGSCFHPGLASTRDGSFG
                     VGDKTAQKRFGTFSEALEYLRSMETAKWRRPNPSGNWGIVSAVRWDKLRK"
     gene            2434..3207
                     /locus_tag="ACHOUK_004332"
     CDS             2434..3207
                     /locus_tag="ACHOUK_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899654.1"
                     /translation="MSKTRLSKPGFSAKSVLTIMLIAASSASYAAESIDQLATRIGMD
                     HFREAIKKGERSAFKTLPAKGYKNGLTGLLIKTNTYVNPEYSFADCDTDTVIISVQEH
                     KTLPGCRISLKLATKDADGSYHDNGIRVEYGIAIGSKKFIANNSAWAQHAISGDKVLE
                     GALHPDKTNNNKDQMNNNKDQMCMGMAQFTANVTEGRNSGMTKREYYQRLDNMNGKAD
                     VTQQMITLYKTFVDYVYEHPKQDAKISGTYIYTNCMNNF"
     gene            3359..4675
                     /locus_tag="ACHOUK_004333"
     CDS             3359..4675
                     /locus_tag="ACHOUK_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899655.1"
                     /translation="MNDGVMLDSDVIAGLEWLASTQENKQHFYQRLAKAQQFYIATTQ
                     KAANFGKQFDPEWYGSDVVAGYFAQAKSLIDNRRSYEVTNASNIIPWVKQLGVCAKSL
                     DRITGARDRAIRMLKNTTVLPDTALLELVLAGNYASEGYEVEFIPEQKGIAKTPEFRC
                     RLGDGDYFFVECKRLQKGPYVKQEILQHHIRSHLAELHIKSKKMNVWTDVTYLCEVKD
                     APENYIISHLKNFKGVFYEWNDDYGKGTIRPANLNLIREDITENGSLLVNTKLARLIK
                     GSPLEDENYQVVAMGRPDERDSRFITKVKLASLITWRCINEKSFDARSRHVTKTLAEI
                     DNQLLDYGLGVGHIALDVDIQKDVADKRREKNYAAIVNFEQKSKIVRLNVHYLVPRID
                     ENSAWMVDETADDFFTHDLVKYVIPLTKIFPEAEVFENDQPGWHQN"
     gene            complement(4874..5443)
                     /locus_tag="ACHOUK_004334"
     CDS             complement(4874..5443)
                     /locus_tag="ACHOUK_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9899656.1"
                     /translation="MAIFGYGRVSTSQQDTENQRMELEQAGWTFDFWFSDVVSGKVPA
                     VQRKAFFEMLSKIRDGETLVVAKLDRLGRDAIDVLQTVRTLADRNIKVIVHQLGTTDL
                     TSAAGKLLLSMLAAVAEMERDLLIERTNAGLLRAKAEGRKLGRPAKIAPEARGAILDK
                     RAAGVSVSALAREYGVSRATLAALLNKGY"
     gene            5636..6103
                     /locus_tag="ACHOUK_004335"
     CDS             5636..6103
                     /locus_tag="ACHOUK_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFP9899657.1"
                     /translation="MIKDAIFSPCGKYRYSLSRVWDESKPYTLFIGLNPSYADAEKDD
                     RTLSRCISFAKSWGYGGVYMANLFAFVHTQRHEMMKASDPIGKDNDSHLIRLVSGAGL
                     VVAAWGNEGRHLKRSTTVRQLLPESTMCFVLNATGEPKHPLYMKNDSVLIPLG"
     gene            7107..7439
                     /locus_tag="ACHOUK_004336"
     CDS             7107..7439
                     /locus_tag="ACHOUK_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HFP9899658.1"
                     /translation="MRTVEILQYTLRKGSGAAFHAIMQEISVPLHQSHGIDVVSFGNS
                     LDDLDCYYLIRAFDSAKSMTAVLDAFYASADWRSGPREDIIGSIENCIKTVISLPSES
                     VKGLRMQS"
BASE COUNT         2155 a         1757 c         1673 g         1945 t
ORIGIN      
        1 tacatcttta aaaatcccga ccaaataatg agatcagcaa acatctattt gaaacgttgg
       61 ttctcatcct caagatcctt gattatttta atatcagaaa attccatacc gccgtatctg
      121 gacttgcagt tgtagtaagt ggcttcagat aagccacatc tttaacagtt cgcccgtatt
      181 caagcgattt aatcacggcg ataatctgat gctcagtaaa aggggcttta cgaatagcga
      241 tctccttcat tggcagattg attatgccgg aggatctcta aatgtgaatg gcacgattat
      301 gcgggatatt tactgtacca gtccaaccct ctgcttactg caataccaat caagagggat
      361 ggtccacatt gacgtttcaa aatcgatttc tgaccagcga gcaaaatgca gcttactgga
      421 ccgaataaag ataagcagag tgagttctgt cgtacagtgg gttagcggtc tgccggtgta
      481 gctgtctatt ttgtcaagca actcagggat acgctttaac tccagcgccg gacgatgctg
      541 tcgattactg gaagcaaccg cccccgacat ctcttgcgcc ggatcgtaat caattaaccc
      601 gctttgcact gcataacgca tgatggccgt ggtgcgctgt tgaaggcggg aagccacttc
      661 aagacgcccg gatttctcca cggctttaat aggaattaac agatcacggg tgcctagttc
      721 agcaatgttg cgagtaccaa ttgtcgggaa gatattgtcc tccaggctct tttttacacg
      781 attagcatga tcttccgacc atttttggtt ggttacgagc cactctctgg caaccttctc
      841 gaaagtaata atctctttcg cctgctcttc tttcacagca cgcttatgct cacgaggatc
      901 gataccagca gctaccagct ttctggcctc ttcccttttc ttccgcgcat ccgccagtga
      961 aacatccggg taaacgccga acgccatcaa atgttgttta ccgccaaaac ggaaacgaaa
     1021 tcgccaatac tttgaaccgc caggttttac aagtaaggac atgccgtcgc cgtcaacaag
     1081 tgaatactct ttctcctcag gtttcgcaga gcgaacttta gtatccgtta gggccataat
     1141 ggtcttcctt ccattggtat ctcgtgagta tacaagcaat atcgaatcgg caaatatact
     1201 cattagtata ctcacaagcc tgttgatgtg ggttggttca ggttgacttc agttgaggag
     1261 aaaagctgga aaagccttgc gtggcgcgga tttcaggcat aaaaaaagac ctcagttgag
     1321 gtctatttac atacttttgg tgccgaaggc cggactcgaa cctaagtctc cataacctgc
     1381 tgtaatcacg ttcctattag cctttaaaag aacaatcgta ggaccttttt gttctagggg
     1441 ctacatctta agtttgatcc gctggacgga atcaaaccga gtgttcgtgg cggcacaaat
     1501 cattaacgtg ccatacctgc cttttgatgt ttatggaggt ctagtgggtc gggaggaaag
     1561 aacatttgtc agctatgtgg atgttaagta aacgccactg cgttcggatt ttgaaaccag
     1621 ttaaaccccg tcagaccttg ttcagaataa ctatttaact caatgttatt tatatgaatt
     1681 aagccattca gcgtcaaagt aaaacgccac cgtaacgcct acgtaacgca cccgcatttt
     1741 gctgttttat tcactcttat agaaacaaag agctgatgaa acgtgaggct atcagcatct
     1801 aatgaacact gttcatccat acagcatgaa aattcataaa cttacacttg agctactttc
     1861 tcagcttgtc ccaccgaacc gcagatacaa taccccagtt gccgctagga ttaggcctac
     1921 gccattttgc tgtctccatg ctacgaagat actcaagagc ttcagaaaac gtgccaaacc
     1981 gcttctgagc cgttttatca cctacgccaa acgagccatc gcgagtgctt gccagcccgg
     2041 gatgaaaaca cgagccatca gctgcataag gaaccagtaa ttccgggtca ctatcttcag
     2101 atgaatcaga caccaatagt cgccacggct caacactcag agcattagcc agcacctcaa
     2161 tcgcatccaa agacgcatta gagccaccgc gttcaatgcg gctcatatag ctacgagcaa
     2221 aaccacaccg atcggcaaag gcttcttggc tcattccggt ggcaatgcgc aactctttta
     2281 ctcgttgtcc aaacagtttt ctcagggtca ttttcatgac ctgaatttgc gcagatgtcc
     2341 actaaagagc cacgctccaa tagtgacatt ttgaatcaat ggtctatgat gcgataactg
     2401 acaccgctgg ggtggattct gagggataac aacatgtcaa aaacacggct tagtaagccc
     2461 ggtttttcag ccaagtctgt gctgaccatt atgttaatcg ctgcgtcatc tgcatcttat
     2521 gcagcagaaa gcatcgatca actggcgacg cgcatcggta tggatcactt ccgggaagcg
     2581 atcaaaaagg gtgaacgcag cgccttcaaa acgctgccgg caaaaggcta taaaaacggt
     2641 cttaccgggc tgttaatcaa aactaacact tacgttaacc ccgaatacag ctttgctgat
     2701 tgcgatacgg acaccgttat tatctcggtg caagaacaca aaacgcttcc cggttgccgt
     2761 atatctttaa agctagcgac aaaagacgca gatggcagtt atcacgacaa tggaattcgc
     2821 gttgaatacg gtattgccat cggctccaaa aagtttatcg ctaacaattc agcgtgggcg
     2881 caacatgcca tcagcggtga taaagtgttg gaaggcgctc tccacccgga taaaacgaat
     2941 aacaataaag atcaaatgaa taacaataaa gatcaaatgt gcatgggtat ggctcaattt
     3001 accgcaaacg taaccgaagg ccgtaacagt gggatgacga aaagagaata ttatcagcga
     3061 cttgataaca tgaacggtaa ggccgacgtc acccagcaga tgataacgct ctataagact
     3121 tttgttgact acgtatatga acatccaaag caagacgcaa agatttcagg gacgtatatt
     3181 tatactaact gtatgaacaa cttctagaaa cgtaggtgcc tgaacatgat agaactcagc
     3241 tgccagcata gagtggaaag cggaggctta ccatcgtata ttcatcgcgt tatctttccg
     3301 taactataat ctgtctcagt tgatctagtg cctttcaggg ctgaactaag gaataaagat
     3361 gaatgacggt gttatgctag attctgacgt tattgctggt cttgagtggt tagcatcaac
     3421 tcaagagaac aaacaacact tttaccagcg cttagcaaaa gcacagcaat tttacatagc
     3481 tacaacccaa aaagctgcca atttcgggaa acagtttgac ccagaatggt atggttccga
     3541 cgtggttgct ggttatttcg cacaagcaaa atcgcttatc gataatagaa ggtcttacga
     3601 agttactaat gcttcaaata taataccatg ggttaaacaa ttaggagtgt gtgctaaatc
     3661 gctcgataga attactggcg ctagagatcg cgcaatcaga atgcttaaga atacaacagt
     3721 attaccagac acagccttat tagagttggt tcttgctgga aactatgcat cagaaggtta
     3781 tgaagttgag ttcattccag aacaaaaagg gatagcaaaa accccggagt ttcgttgccg
     3841 ccttggagat ggcgattatt tctttgtcga atgcaaaagg cttcagaaag gtccttacgt
     3901 aaagcaggaa atattacagc atcatatacg ctcacatctt gccgaacttc atatcaaatc
     3961 caaaaagatg aacgtatgga ctgatgtcac atatttatgt gaagttaaag acgcgcctga
     4021 aaactatatc attagccact taaaaaactt taaaggtgta ttttatgaat ggaatgatga
     4081 ttatggaaaa ggaacgatta gaccggccaa cttaaatctt atcagggaag atatcactga
     4141 aaacggctca ttactagtca acactaaact tgctcgtctt atcaaaggct cccctctgga
     4201 agatgaaaat taccaagttg ttgctatggg acgaccagat gagcgcgact cacgtttcat
     4261 aaccaaagtt aaacttgctt ctttgataac atggcgttgt atcaatgaaa aatctttcga
     4321 tgcacgttcg cgacatgtaa caaaaacatt agcagagata gataaccagc ttttggatta
     4381 tggtctaggc gtagggcata tagctttgga tgtagatatt caaaaggatg ttgccgataa
     4441 acgaagggaa aaaaactatg ctgccatagt aaattttgag caaaaatcta aaatagtccg
     4501 cctgaatgtt cattatctcg tgccacgcat agatgagaac tctgcgtgga tggtagatga
     4561 aactgcggat gactttttta cccatgatct ggtcaaatat gttatcccat tgactaaaat
     4621 attccccgag gctgaggtat ttgagaatga ccaacctggc tggcatcaaa attaatttat
     4681 ttttgatagt atgaacgagg gtagcatttc tatacaaatc agtgttcggc tgatagcgag
     4741 ttaaacagtt tactgaaatg ttctcagcaa ttagctgttg ccaggacctc aaaaatccga
     4801 ttctggcgga aagtgtgctg ttttaccaag tattgctcgt atctgcaaag tgttagagca
     4861 tgttttaagt atatcagtaa cccttattca gtaatgcagc aagcgtcgct cttgaaacac
     4921 cgtattcacg cgccaatgcg ctaacactaa ccccagcggc ccttttgtct aaaatcgctc
     4981 cacgagcttc cggtgcgatt ttagcaggcc tgcccaattt cctgccttca gcttttgccc
     5041 gtaacagccc cgcattagtc cgttcaatca gcaggtcacg ctccatctcc gcaaccgctg
     5101 ctagcatcga caacaggagc ttaccggcag cagaggtgag atcagtggtg ccaagctgat
     5161 ggacaataac cttgatgttt cggtcagcca acgtcctgac agtttgcaga acgtcaattg
     5221 catcacgccc gaggcggtcc agctttgcaa ctaccaacgt ttccccatcg cggattttgc
     5281 tgagcatttc aaaaaacgct ttgcgttgca ccgcaggaac ctttccgctg actacatcag
     5341 aaaaccaaaa gtcgaacgtc cacccagctt gttcaagttc catgcgctgg ttctcagtat
     5401 cctgctggga tgtcgaaaca cgcccgtagc caaaaatagc catagtcatc tcctcatccg
     5461 gcccctgcca gaaaacggtg atgaaaatag gatggcctgc cggaaaagcc aaggctaatt
     5521 ttccaacagt gtgaaagcgt gcggttggag gatgccggaa aacggtcgtt tgttggcgct
     5581 taaaacgggt gaagacatgt taggctgaaa ccatcacttc ataaggacaa ccgaaatgat
     5641 taaagacgcg attttttcac cgtgcggtaa gtatcgctac tcgttatcac gagtctggga
     5701 cgaatcaaaa ccgtataccc ttttcatagg gctaaaccct tcatacgccg atgccgagaa
     5761 ggatgatcgc accttgagcc gctgcatatc gtttgccaaa agctggggct acggtggcgt
     5821 atacatggcc aacctgttcg cattcgttca cacccagcga catgaaatga tgaaagcgtc
     5881 tgatcccatt ggcaaagata acgatagcca cttgatacgt cttgttagcg gtgccggact
     5941 ggttgtcgcc gcatggggaa atgaaggaag acacctcaaa cgctcaacga ccgtccgaca
     6001 gctattgcca gagagcacaa tgtgcttcgt cttaaacgct acaggtgagc cgaagcaccc
     6061 tttgtatatg aaaaacgaca gtgttctcat acctttaggt taacatcgat aactgcgttc
     6121 agaatatgaa cgcagccata tgatatatat gacttttctt taaaatgatg gtgtgctagc
     6181 ttctctcctg acacctacct catgcggtaa accggtccgg gtaggtggag atcaccgctg
     6241 tttagcggaa gacgtcagga tgggaactct gcgtaacgtt gattgtggta ggtagggcgc
     6301 gcaagcgttt gatgcagcca agcagagggt gaaagtagct atcggcggat tcttaagtag
     6361 cttcttttgc gcataatgca tctttcaaga aaggtcgtca caccacgagg aagacttgat
     6421 cttcatctgg caaggggaac cgagcaaacc tacaggctat cttctcagat ggccttaggg
     6481 ttgatatgtc cggcttcccc cccgaggaag ggtttacatc ttaaagaaca agttatgttt
     6541 taacattttt aacaattaca cttctctgct gtttggtgtc ttatggcgtt cccgtcaaaa
     6601 gtaaagctgc cgtttttacc tcacatgcgt aacaaccaaa cacgaaccag aagctgctaa
     6661 cgttcaacgt gttcgcggtg taaatgcagc cggataagtt gggagcgaaa aagtggatgt
     6721 cctaggtttg gaagcgcccc tcttaagcat tgcatccgat aatttccgtg gcggtgagac
     6781 agtccttata gaggcaatca gcctccaaag ttaagcgacg cggtaaaact aaagctcgcc
     6841 cagatttctc attgattgct cgaactccgc tacgcttgtt tcaactggct tcaccagtca
     6901 ctctatgaga gcgccgcgaa ttctgcacca tgatttcgac taccttcttt aaaacactcg
     6961 ccagagagcc ttacagcgcg tttgaaccgc atatcgcagc ttctgcttac ggatgactgt
     7021 ctgattagca gtagcttcat ggcccagtcc ggtttacttt attgctatca actaggagcc
     7081 gcccaagggc aacacggaga taacgcttga gaaccgtcga aatactgcaa tacaccttac
     7141 gcaaaggcag cggtgccgct tttcacgcca taatgcagga aatcagtgtt cccctacacc
     7201 aaagccacgg gatcgatgtt gtttcgtttg gcaactcgct tgatgacctg gattgctact
     7261 acttgattcg cgcctttgac agcgctaaaa gcatgactgc ggttctcgat gctttttacg
     7321 caagcgcaga ctggcgcagc ggcccacgcg aagacatcat tggcagcata gagaactgca
     7381 ttaaaacagt gattagccta ccatcagaaa gcgtgaaagg gctgcggatg caatcgtaag
     7441 taatcaagtg tattagctta gatttgacct aaacagcttt gtgccaaaag tggactagga
     7501 aaagcctagc gataaagagt tgagtttaat
//
LOCUS       DAZKIP010000177         7482 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZKIP010000177 DAZKIP010000000
VERSION     DAZKIP010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.179"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..384
                     /locus_tag="ACHOUK_004337"
     CDS             <1..384
                     /locus_tag="ACHOUK_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFP9899659.1"
                     /translation="LAEFVALISESGANPFGLTVDAVMEEYRRWRNESWRYDGSDKYP
                     WPQPVLYHICLEMRSKGIERQMTEGELKRLVERQLTKWAKHVGNGLSVPPVRRQLAAP
                     KRPPGPTPIELLKQEYERRKAAGFV"
     gene            419..1117
                     /locus_tag="ACHOUK_004338"
     CDS             419..1117
                     /locus_tag="ACHOUK_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFP9899660.1"
                     /translation="METVFDALKALKKASSHEIAARLEISRDDAVTELWKLKRRGEAD
                     NKGSMWWLTSEATEAAPKTTAEMLINAIEQHGPQSADELALMFGITSRRANSSLAMAV
                     SKGRLIRVNQDGKYRYCIPGDNLPAEPKAASVTETDGKAFPQPAGVALPVREAETQEE
                     IKTESVAVTVQSQSSFIRKHPDGLILPSLHVANRELRRAKGQVQKWERLCAALREINK
                     HRDVIQKITTGDNG"
     gene            1139..1363
                     /locus_tag="ACHOUK_004339"
     CDS             1139..1363
                     /locus_tag="ACHOUK_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899661.1"
                     /translation="MKIRYHDFGPVSHMLISSTVLETRKHNHILDMLRLADPYLVINT
                     SGIFFLRSTVSGKTSHVLRAYKTAVREEGE"
     gene            1360..1716
                     /locus_tag="ACHOUK_004340"
     CDS             1360..1716
                     /locus_tag="ACHOUK_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eae-like protein"
                     /protein_id="HFP9899662.1"
                     /translation="MSEINYQELRVELGAAKKRIEELEANRVVLEVENERLKHAMAVA
                     LEHISVTDAGQAGVAAMIIYDAMYHSEKTDPYAFLAELRAQGVEMVREHPAIKLCSLT
                     HICDELVAELRKGGNK"
     gene            1749..1901
                     /locus_tag="ACHOUK_004341"
     CDS             1749..1901
                     /locus_tag="ACHOUK_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899663.1"
                     /translation="MAKKHHYFAEGEITSICGGWMYFGNEREPDTFESPDDCKKCRRK
                     LNKGGK"
     gene            1898..2209
                     /locus_tag="ACHOUK_004342"
     CDS             1898..2209
                     /locus_tag="ACHOUK_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899664.1"
                     /translation="MSVIKEMPVERNEYGCWTHPEYEKFCDGREYISTEEFNAWMEKN
                     NLQWAIRSMDEDDFNLDADGPDISAWEPERPEGEGWFIGSIHDTEDGPVCVWLRNKAE
                     A"
     gene            2336..2899
                     /locus_tag="ACHOUK_004343"
     CDS             2336..2899
                     /locus_tag="ACHOUK_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899665.1"
                     /translation="MTELTKEWLQNTITGIESSRDEIPFGLDEDQNNMLTALKIALAS
                     LASVSDERAAYELFMEKRFGESVDRRRAKNGDREYMAWDMALGWIIWCHRAAMLQAGN
                     FRENKGSSTNNFRIISETSTNSPAIPDEVLSAILKVARLRADFDASEVDRRGIGSCLD
                     EAEQELIVTINEYASQIAVEATQGENQ"
     gene            3009..3113
                     /locus_tag="ACHOUK_004344"
     CDS             3009..3113
                     /locus_tag="ACHOUK_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899666.1"
                     /translation="MSDDISLVMEGALAVIAVVGVYCLVVFLMERLGN"
     gene            3357..3512
                     /gene="hokD"
                     /locus_tag="ACHOUK_004345"
     CDS             3357..3512
                     /gene="hokD"
                     /locus_tag="ACHOUK_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFP9899667.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            3680..3958
                     /locus_tag="ACHOUK_004346"
     CDS             3680..3958
                     /locus_tag="ACHOUK_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899668.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDWYQHDPCTEEQAEW
                     LIQCYRRRGYEVKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            3960..5009
                     /locus_tag="ACHOUK_004347"
     CDS             3960..5009
                     /locus_tag="ACHOUK_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP9899669.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIRHAMNGTQERE
                     LSLAELSWWAACNQVVDALPEAVARRSLGLPAEKIRSVYRESDIVPGEQTAISILKQR
                     TKNIALPLHVHQQQNPPQKKTVVSIAVDPESPESFMRRPKRCRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKAHDIFTLPLCREHHNELHADPLEFEKKYGSQIEL
                     IFRFLDHAFATGVLG"
     gene            5022..5381
                     /locus_tag="ACHOUK_004348"
     CDS             5022..5381
                     /locus_tag="ACHOUK_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP9899670.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVRGQ
                     LVPGERLGIKITELECA"
     gene            5378..6067
                     /locus_tag="ACHOUK_004349"
     CDS             5378..6067
                     /locus_tag="ACHOUK_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFP9899671.1"
                     /translation="MNNHYLQFVRELLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKVTSIPLVSPVEFSTSSWRRAVLSLEEHHK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNTQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYKELALLVGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKQKA
                     AFSKQSVAKVD"
     gene            6278..6353
                     /locus_tag="ACHOUK_004350"
     tRNA            6278..6353
                     /locus_tag="ACHOUK_004350"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6311..6313,aa:Ile,seq:cat)
     gene            6361..6437
                     /locus_tag="ACHOUK_004351"
     tRNA            6361..6437
                     /locus_tag="ACHOUK_004351"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6395..6397,aa:Arg,seq:tcg)
     gene            6451..6527
                     /locus_tag="ACHOUK_004352"
     tRNA            6451..6527
                     /locus_tag="ACHOUK_004352"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6485..6487,aa:Arg,seq:tct)
     gene            6701..7129
                     /locus_tag="ACHOUK_004353"
                     /pseudo
     CDS             6701..7129
                     /locus_tag="ACHOUK_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310776.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
     gene            7126..7293
                     /locus_tag="ACHOUK_004354"
     CDS             7126..7293
                     /locus_tag="ACHOUK_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFP9899672.1"
                     /translation="MTVKLRLTVAALLLFLVVMVDFTSRIMSVLADGVLVCGIMVLLW
                     PVIKRNSLHNA"
BASE COUNT         1970 a         1662 c         2094 g         1756 t
ORIGIN      
        1 ctggctgagt ttgtggcgtt gatttcggag agcggggcaa atccatttgg cctgacggtg
       61 gatgccgtga tggaggagta ccgtcgctgg aggaatgaat cctggcggta cgacgggagt
      121 gataaatacc cgtggccaca gccggtgctg taccacattt gcctcgagat gcgttcaaag
      181 gggattgagc gccagatgac cgaaggggag ttaaaacggc ttgtggaaag gcagctgacg
      241 aaatgggcaa agcatgttgg taatggcctc agtgttccgc cagtccggcg acaactggca
      301 gcacccaaac gcccgccagg gccaacgcca attgaattgc tgaaacagga atatgagcgc
      361 cggaaggcgg ccggttttgt ctgagttgag aagtaatttt taccgggagg aaaatttaat
      421 ggaaaccgtt tttgacgcac tgaaagcact gaaaaaagcc tcgtcgcacg agattgcagc
      481 ccgtcttgaa atcagccgtg acgatgctgt taccgaactc tggaagctga agcgtcgtgg
      541 cgaagctgat aacaaggggt cgatgtggtg gctgacgagt gaggcaactg aagcggcccc
      601 aaaaaccact gcagagatgc tgattaacgc gattgaacag catggtcctc agtcggctga
      661 cgaactggcg ttaatgttcg ggattacctc ccgccgggcg aattcatcac tggcgatggc
      721 tgtcagcaaa ggtcgtctga ttcgcgtaaa tcaggacggt aaatatcgtt actgcatacc
      781 gggcgataat ttaccggcag agccgaaagc tgcatcggta acggaaaccg atggtaaagc
      841 ctttcctcag ccggcaggtg ttgcgttacc agtccgggaa gcggaaacac aggaagaaat
      901 taaaactgaa agtgtggcgg tcacagtgca gtcacagtcg tcgttcatca gaaagcatcc
      961 ggatggtctg attttaccat cgctgcatgt ggctaaccgc gagctgcgcc gggcaaaagg
     1021 tcaggttcag aagtgggagc gcttatgcgc cgcgctgcgg gaaattaata agcatcgtga
     1081 tgtcatacag aaaataacga caggagataa cgggtaattt ttacagcagg gtgatgatat
     1141 gaaaatcaga tatcatgatt tcggccccgt gtcacatatg cttatttcaa gtacggtgct
     1201 ggaaacaaga aaacataatc acatactgga tatgctgcgt cttgctgacc cgtatctggt
     1261 aataaacacc agtggaattt tctttctgag aagcactgtg tcaggaaaaa catcgcatgt
     1321 gcttcgggca tataaaacag cagtgcggga ggaaggagaa tgagcgagat taactatcag
     1381 gagctgcgtg ttgagctggg agcagcaaaa aaacgcatag aagagctgga ggctaatcgt
     1441 gtggtgcttg aggtggaaaa tgagaggttg aaacacgcaa tggccgtagc ccttgagcat
     1501 atatccgtca cagatgcagg acaggcaggt gtcgctgcaa tgattatcta tgatgccatg
     1561 taccacagtg aaaaaactga cccctacgct tttctggcag aattgcgggc gcagggtgtg
     1621 gagatggtgc gcgaacatcc agcaatcaaa ctttgctctt tgacgcacat atgtgacgag
     1681 ttagtcgcag agcttcgcaa aggaggcaac aagtgagtaa aacgaatccg ggctgggcaa
     1741 ggccacttat ggcgaaaaaa catcattatt ttgctgaagg tgaaataaca agtatttgcg
     1801 gtgggtggat gtattttggc aatgagcgtg agccggatac attcgaaagt ccagatgatt
     1861 gtaaaaagtg tcgtagaaaa ttaaataagg gggggaaatg agcgtaatta aagaaatgcc
     1921 agtggaacgt aatgaatatg gctgctggac gcatcctgaa tatgaaaaat tctgtgatgg
     1981 tcgggaatat atttcaacgg aagagtttaa cgcctggatg gagaaaaata atcttcagtg
     2041 ggctatccgt agtatggatg aagatgattt taatctggac gcagatggcc ctgatatttc
     2101 agcatgggaa cctgagcgac cagaaggtga gggatggttt atcggttcga tacatgacac
     2161 cgaagatggt cctgtttgtg tatggctgag aaataaggcc gaagcataaa ggctataaac
     2221 caacaactaa acactgaaaa tttaaatcag aaatgatttt tattaaatcc ttaaccggag
     2281 ggattcctgc accctcagaa catcaggaga ccgcccgaaa gggcggtagt gaaaaatgac
     2341 tgaattaacc aaagaatggc tacagaacac gattaccgga attgagtcat cacgggatga
     2401 aataccgttc ggactcgatg aagatcaaaa caacatgctt accgcattaa aaattgcact
     2461 ggcatcactg gcatcagtat cggatgaacg ggcagcctat gaattattta tggagaagcg
     2521 tttcggggaa tctgtagatc gccgcagagc aaaaaatggc gatagagaat acatggcatg
     2581 ggatatggcg cttggctgga ttatctggtg tcaccgcgcc gccatgcttc aggctggaaa
     2641 ctttcgggaa aataagggtt cgtcaaccaa taattttcgg ataatctcgg aaacgtcaac
     2701 caactctccg gcaatccctg atgaggtgtt gtccgcaatc ctgaaggtcg ccaggcttcg
     2761 tgcagatttc gatgcttctg aagtggacag gcgaggtatc ggtagttgtc tggatgaggc
     2821 cgagcaagaa cttatcgtta ccattaacga atacgccagt cagatcgcag tagaagcgac
     2881 acagggggag aaccaatgag ccggccagat gcatttgcag gcgttgctat agctattgct
     2941 tttctagtat atgttatttg tcggtggggg taaaaacgtt cgccgggatt cacaccaagg
     3001 gagggaatat gtcggatgat atttcactgg taatggaagg cgctctggct gttattgctg
     3061 ttgtgggtgt ttactgcctg gttgtgtttt tgatggagcg cctggggaac tgaattcatt
     3121 ccgtatggga attcccatat cgggcaaaac ggtttgctgt aaagcgagag ttaagtagaa
     3181 ttgctgcggg tgcttgaggc tgtctgcctc gggcatgcca ccgtaaggca gacagagaaa
     3241 agccccagtt aacattacgc gtcctgcaag acgcttaaca ttaatctgag gcccaatcta
     3301 tgcttcacaa acgtaggtta gcctcttacg cgccgaaagg caaggagaag caggttatga
     3361 agcagcaaaa ggcgatgtta atcgccctga tcgtcatctg tttaaccgtc atagtgacgg
     3421 cactggtaac gaggaaagac ctctgcgagg tacgaatccg aaccggccag acggaggtcg
     3481 ctgtcttcac agcttacgaa cctgaggagt aagagaccag gcgggggaga aatccctcgc
     3541 caccgctgat gtgtcaggca tcctcaacgc acccgcactt aacccgcttc ggcgggtttt
     3601 gttttttctg gtcgttctgg tttacaatcc atccgtcagc ctgaacaact ggcacctgct
     3661 gcgccagcag agaaaacaga tggcgcacga taccaaattt tacaattcgg ataactctgc
     3721 cgcccctgcc agcaggcacg ggcggcgttc tcatgcattc aaatctgact ggtatcagca
     3781 cgacccctgc accgaagaac aggctgaatg gctgattcag tgttaccgca ggcgcggata
     3841 cgaggttaag aaagccctca gtctcgatta tcgtcactgg ataatctccg tcaggcttcc
     3901 ttactccgaa cgcccaccgc gtccgtcccg cacattccag caacgcatct ggaggtaacg
     3961 tgcgggtatt acttcgacct gttctggtac cggaactcgg gctggtgatc gttaagccgg
     4021 gccgtgaatc catgccggta ttccacaata cccgggtact ggtggagccg gaaccgaaaa
     4081 gcatgcgtaa tctgccgtcc ggggtcgttc ctgccgttcg ccagccgctg gcggaggata
     4141 aatcattact gccatttttc agcgacgaac gagtgattcg tgctgctggt ggcgctggcg
     4201 cactgtctga ctggttactg cgccatgtta aatcctgcca gtggccacac ggcgattatc
     4261 accacagtga aaccgtcatt caccgttatg gtaccggcgc aatggtgttg tgctggcact
     4321 gcgacaacca gttgcgtgac cagacctccg aatcacttga gcaacttgct caccaaaacc
     4381 tgtcagcatg gatgattgac gtcattcgtc acgcaatgaa tggcacacag gagcgtgaat
     4441 tatcgctggc tgaattatcc tggtgggcgg cctgcaatca ggtggtggat gcactacctg
     4501 aggcagtagc gcgtcgttcg ctgggattac cagcggaaaa aatccgctcc gtataccgtg
     4561 agagtgacat cgtaccggga gaacagacag ccatcagcat actgaagcag cgcacaaaaa
     4621 atattgcgct gccacttcac gtccaccagc aacaaaatcc accacagaaa aaaacggttg
     4681 tcagtatcgc cgttgatccg gagtctcctg aatcgttcat gaggcggcct aaacgttgcc
     4741 gctgggttaa tgagaaatac acgcgctggg taaagacaca gccgtgtgcg tgttgtggta
     4801 agcctgctga cgatccgcat cacctgattg gtcatggtca ggggggaatg gggacaaagg
     4861 cccacgatat tttcacgcta ccgttgtgcc gggagcacca caacgaactt catgcagacc
     4921 cgctggagtt tgagaaaaag tacggctctc agattgagtt aatttttcgt tttcttgatc
     4981 acgcctttgc gactggcgtg ctcgggtaaa agaggtgact gatgctcata gatttggttt
     5041 taccttaccc gccgacggtg aacacctact ggcgacgtcg tggcagcaca tattttgtat
     5101 caaaagccgg tgagcgttat cgccgggctg tggcgcttat tgttcgccag cagcggctga
     5161 aattaagcct gtccggaagg ctggcgataa agattattgc cgagccaccg gataagcgcc
     5221 gccgtgacct ggacaatatt ctgaaagcgc cgctggatgc gctgacgcat gcggggttgc
     5281 taatggacga tgagcagttt gatgaaatca atattgtgcg cggtcagctc gttcctggtg
     5341 agcggctggg gataaaaatc acagaactgg agtgcgcatg aataaccact atttacagtt
     5401 tgtgcgtgag ctgctcatta tcgctaccgc cgatttgagt ggggcaacaa aaggtcagct
     5461 tgaagcctgg caggagaatg ccatgttcga tacagggcgt tacaggcgaa aaaaaatccg
     5521 gtaccgcgat gaagtgactg gaaaaatgat aacgcgggat aatccaccaa tcccgggaaa
     5581 gcaatcgctg gcgaaggtga cgtcaattcc tctggtcagt ccggttgagt tttcgacatc
     5641 atcgtggcgg cgggctgttc tgtctcttga agaacatcat aaagcctggt tgttgtggtg
     5701 ttacagcggg agtatttgtt gggaatatca gatcgcgata acacagtggg cgtggaatga
     5761 atttaatact caatccggta ccagaaaaat tgcagggaaa acgcaggaac gcctgaaaaa
     5821 attaatctgg ctggcggcgc aggcagtaaa agcagaactt tttggtgggg aaggttatga
     5881 atacaaggag ctggcattac tggtgggagt gacaactaaa aactggtcca aaacatttac
     5941 tcgtcactgg gttgcaatga aacacatttt tcaccgactg gatagtgagg ctttattgtt
     6001 tgtaatgaga acgcgttcaa aacaaaaggc ggcattttca aagcaaagtg ttgcaaaagt
     6061 agattgaaag gcatatattt catgcaaatc tgatattttg ccgattttgt acgtgatggc
     6121 aaaagcaaac aaaacccgcc cacaagcggg tttttttgtg ccacttatct cggatagaca
     6181 tggtgaatgc gctggtggag gaagcaaggg taatttttaa ccaggtgatt cttgaatgct
     6241 tgcaacattg atttcgtaac gttattatcc tgcgcccggc cctttagctc agtggtgaga
     6301 gcgagcgact cataatcgcc aggccgctgg ttcaaatcca gcaagggcca ccatcacata
     6361 ccgccattag ctcatcggga tagaacgcca gccttcgaag ctggtttcgc ggggttcgag
     6421 tctccgatgg cggtccatta tcggtattct gcgtttttag ctcagccgga cagagcaatt
     6481 gccttctaag caatcggtca ctggttcgaa cccagtacaa cgcaccacac ttattttccc
     6541 tcgctcgctt ttgcggacct tttttgtatc cgcaccacgc ccggcgcata ccaaccacag
     6601 agcctttcgg ggggagctta tggagtggtc agtgtgactt tctctgtggg cagatcgctc
     6661 ccgggcgttg gctcacccac ccaaaggaac gtcacgatgt ttggtatttt tggtaaaaaa
     6721 agcccgcaga gcggcaacgg aaattaaaaa gtttgaaaaa cgcgatctgg cacaggtggt
     6781 gattaacgcc gcatacctgg tggcctgtgc agatggtgaa tgtgaggctt cctagaaagc
     6841 gaagatcgaa caggtactgc gtaatcagcc tgcgctgtac gcgtttacgt cagaaattaa
     6901 tgcgattagc gcaaccatta tcggtcagct ggatacgaac tttaaaactg gtcgtcgtgc
     6961 ggcgttacgt gagatcgagg atgtgaaaca cgatacgcgt gaagcggaag atgtgctgga
     7021 tgtggcggtg gccattgcgg aggcagacgg cgaaattgag ccggaagagc gcaaggtgct
     7081 ggaagagatt gccggtgttc tgggtcttcg tctggagaat tacctgtgac ggtaaaactg
     7141 cgcctgactg tggctgcact cctgctgttt ctggtggtga tggtggattt caccagcaga
     7201 atcatgtcgg tgctggcgga tggggtgctg gtctgcggca ttatggtatt gctgtggccg
     7261 gtgataaaaa gaaacagcct gcataatgct tgattttttt gtttgctgtt tattaaaaac
     7321 acttctgcat ggtgaatccc cctgtgcggt ggggcaatca gcaagaagga atatggggta
     7381 atcgcggatt caggtgctgg tactgaattc accgggaggc acccggcacc atgctttgcc
     7441 acaaaagtgt tatttctgtt tttctcaaac tatcatcgtt at
//
LOCUS       DAZKIP010000178         7453 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZKIP010000178 DAZKIP010000000
VERSION     DAZKIP010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.180"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            23..1789
                     /gene="adeD"
                     /locus_tag="ACHOUK_004355"
     CDS             23..1789
                     /gene="adeD"
                     /locus_tag="ACHOUK_004355"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFP9899673.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYTDAPALQRIDAHGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESAKLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFERNDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(1835..3226)
                     /gene="uhpT"
                     /locus_tag="ACHOUK_004356"
     CDS             complement(1835..3226)
                     /gene="uhpT"
                     /locus_tag="ACHOUK_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFP9899674.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3364..4683)
                     /gene="uhpC"
                     /locus_tag="ACHOUK_004357"
     CDS             complement(3364..4683)
                     /gene="uhpC"
                     /locus_tag="ACHOUK_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFP9899675.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVIAIAAGISALLLLPFLNAQTPREA"
     gene            complement(4693..6195)
                     /gene="uhpB"
                     /locus_tag="ACHOUK_004358"
     CDS             complement(4693..6195)
                     /gene="uhpB"
                     /locus_tag="ACHOUK_004358"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFP9899676.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFSLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIEESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWLQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLTISCLHGTRVSVSLPQRYV"
     gene            complement(6195..6785)
                     /gene="uhpA"
                     /locus_tag="ACHOUK_004359"
     CDS             complement(6195..6785)
                     /gene="uhpA"
                     /locus_tag="ACHOUK_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFP9899677.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6947..>7453)
                     /locus_tag="ACHOUK_004360"
     CDS             complement(6947..>7453)
                     /locus_tag="ACHOUK_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899678.1"
                     /translation="DLVRDSLVQDGIKPEKIAKIISCLSSRTGIRNLFYACSQAFHVA
                     NDNAALDVRRAATSVGDTIDVCPSFVALNGRNYHQHVDFSEYGFTQTLCVLGAVVNNA
                     NDTFDVANYIYTTKGENIIHNDAEDDSPYVHSDESYRHKDDYWARDNDIRNLRRNFSG
                     NITIATYK"
BASE COUNT         1913 a         2096 c         1900 g         1544 t
ORIGIN      
        1 ataaaatcaa aatgagaaaa atatgaataa ttccattaac cataaatttc atcacattag
       61 ccgggcggaa taccaggaat tgttagccgt ttcccgtggc gacgctgttg ccgattatat
      121 tattgataat gtctctattc tcgacctgat taatggcgga gaaatttccg gcccaattgt
      181 gattaaaggg cgttacattg ccggtgttgg cgcagaatac actgatgctc cggctttgca
      241 gcggattgat gcccacggcg caacggcggt gccagggttt attgatgctc acctgcatat
      301 tgaatccagc atgatgacgc cggtcacctt tgaaaccgcc accctgcctc gcggcctgac
      361 aactgttatt tgcgatcctc atgaaatcgt caacgtgatg ggcgaagccg gtttcgcctg
      421 gtttgcccgc tgtgccgaac aggcaaggca aaaccagtac ttacaggtca gctcttgcgt
      481 acccgctctg gaaggctgcg atgttaacgg tgccagtttt acccttgaac agatgctcgc
      541 ctggcgggac catccgcagg ttaccggcct tgcagaaatg atggactacc ctggcgtaat
      601 tagcgggcag aatgcgctgc tcgataaact ggatgcattt cgccacctga cgctggacgg
      661 tcactgcccg ggtttgggtg gtaaagaact taacgcctat attgctgcgg gtattgaaaa
      721 ctgccacgaa agttatcagc tggaagaagg acgccggaaa ttacaactcg gcatgtcgtt
      781 gatgatccgc gaagggtccg ctgcccgcaa tctcaacgca ctggcaccgt tgatcaacga
      841 atttaacagc ccgcaatgca tgctctgtac tgatgaccgt aacccgtggg agatcgccca
      901 tgaaggacac atcgatgcct taattcgccg cctgatcgaa caacacaatg tgccgctgca
      961 tgtggcatat cgcgtcgcca gctggtcgac ggcgcgccat tttggtctga atcacctcgg
     1021 cttactggca cctggtaagc aggctgatat cgtcctgttg agcgatgcgc gtaaggtcac
     1081 ggtgcagcag gtactggtga aaggcgaacc gattgacgcg caaaccttac aggcggaaga
     1141 gtcggcgaaa ctggcacaat ccgctccgcc atatggcaac accattgccc gccagccagt
     1201 ttccgccagc gactttgccc tgcaatttac gcccggaaaa cgctatcggg tcattgacgt
     1261 catccataac gaattgatta cgcactccca ctccagcgtc tacagcgaaa atggttttga
     1321 gcgcaatgat gtgtgcttta ttgccgtact tgagcgttac gggcaacgtc tggctccggc
     1381 ttgtggtttg ctcggcggct ttggtctgaa tgaaggcgcg ctggctgcga cggtcagcca
     1441 tgacagccat aatattgtgg tgatcggtcg cagtgccgaa gagatggcgc tagcggtcaa
     1501 tcaggtgatt caggatggcg gcgggttgtg cgtggtacgt aacggccagg tccaaagcca
     1561 tctgccgtta cccattgccg ggctgatgag caccgacacg gcgcagtcgc tggcggagca
     1621 aattgacgcc ttgaaagccg ccgcccgtga atgcggtccg ttacccgatg agccgtttat
     1681 tcagatggcg tttctttccc tgccagtgat ccccgcgctg aaactaacca gccaggggct
     1741 gtttgatggc gagaagtttg ccttcaccac gctggaagtc actgaataat aaaaaaagcc
     1801 cggcgtcatg ccgggcaaaa gtcaccagtt acgtttatgc cactgtcaac tgctgaattt
     1861 ttttctcgcg gcggattttg cgttcttcca tcaccgccac tatcgccatc aggcagatac
     1921 aaccaatcgc ggcgatatcc agcgcagcga aggtgcctgc ccagccggta aggccgaata
     1981 ctggcgtccc atcggcaatc attcccagac ctaacttggc aaagctgtca ccaatcaggt
     2041 aagcgaaggt gcctttaata ccatcggcag cgccaatcgc ttttttaggt acaaagccaa
     2101 cagcagccac accaatcaac aattgcgggc caaagaccag gaaacccaac gcaaagagag
     2161 aagccagata gatatattcg ttgctggcat gttggtacac accgagcgtg gcgataatca
     2221 gcgccagcgc gatgcaggcc accaggccac ggcgaccgtt cgctaggtca gagagccagc
     2281 cccacagcag cgtaccgacc agcgcaccag cttcaaacag cgtaaagccc tgaatcgcca
     2341 ccgctttaga gagtttcagt tcctggaacg cgtacacagt tgaccactgg tcgataccaa
     2401 tacgcaccac atagaggaaa atgttggcga agcacagcag ccagatcact ttgtttttca
     2461 gcacatactc aacaaagatc tgccacttgg tcatatcggt agattctgtc tctttgtcct
     2521 cttcgctgat ctcctcgccg aacagctctt cagctttgcc gaggccataa gattccgggg
     2581 agtcgctgcc gtaacgcagg ccgataaaac cgacaatcag cgcgataatc gacgggaaga
     2641 taaacatgcc gatgacatgg ccatcgaaca ggtaatttgc gccgaacagt gccacaccag
     2701 ctgcgcctgc accgccaagg ttgtgagaaa tgttccagaa accgaggaac gtcccgcgtt
     2761 tacgacgcgg cgtccatttg gtgatggtgg agtaactgca cgaacccccg gtactctgga
     2821 aaaagccact taaggcgtag aaggcaatca tcaggaacag gctaaccgag ccgctgccca
     2881 tactggcgct aaagcccagc atacaaatgg cagacaggat cagcatgaac ggcaggaatt
     2941 gtttggtgtt tttgccgtcg gcgtagtagg aaaccagcgt tttacccacg ccataagtga
     3001 tggagaaacc caggccgatc atccccagct gcgtcatgct caacccgtag gtcgaaatca
     3061 tatcgttctg cgcgatgtta aagttcttgc gaatcaggta catcgtcagg tagccgataa
     3121 agaccaccag gtaggattgc atgaacggtt tgaaccacat tttgcgccgc acttcgagcg
     3181 gaaggtccag ggtcggcttg cggacctggt ttaagaaagc cagcatgggt tactcctgaa
     3241 atgaatacct gcgtgaggca tgcattgtaa aaatcagcag caaatcaggc ctgagatagc
     3301 gtccaggcaa aacctggaaa atttcttagt tttgccggat atgaagtgaa aaggtgagat
     3361 gcatcacgct tcgcgcggtg tctgggcgtt caaaaagggc agtaacagca gtgcggaaat
     3421 cccggcggca atggcgataa ccacaaaaaa tccgctccag tgccaggtat cgagcacttt
     3481 cgccagcggc caaccagcaa gcgacgcccc cagataagca aacaagccga caaaccccgt
     3541 cgccgcccct gccgcctctt tgtgggaaca ttctgccgcc gccatgccga ttaacatctg
     3601 cgggccaaag acaaaaaaac caatggtgaa gaagcaggtt gcctgcatca cgtagctggc
     3661 aaatggcatc agccacagcg agccgactga aagcaaaatt ccggcagcga aaatcaaatt
     3721 catcggcccg cggttgccgt taaacagttt gtccgagccc caacccgcta ccagcgcacc
     3781 gataaatccg cccagttcaa acatcgtcac tgccgtattc gccgtgacca gatcgacgcc
     3841 cagcgtctcg gacatataca aattgcccca gtcgttgatc gccgcccgaa ccacatagac
     3901 cagcacatag caaaacgaaa gcagccagat atacggattc aacaacacat acttggtgag
     3961 gatctcttta cgcgtcagcc ctgccccttc ttgttgttga gcaatctcca gcgcgtcgtg
     4021 tcgccattca ccgaccgctg gtaaacctaa cgcctgcggg cgatcgcgca gccgccagca
     4081 gagaaaaatc cccacgacga tcgccataca accagcaatc atcatcccgg cacgccagcc
     4141 gtaatgcagc gcagctgctg ccatcacaat gggaatgagc gcaccgccaa cgttatgcgc
     4201 cgtgttccat aatgcccacc aaccaccgcg ttcggtacga gaataccagg ccgttaacag
     4261 acgcgcacac accggtgaac cccagccctg gaaaaaggcg ttcagcaccc agagcacggc
     4321 aaacgcccat agcgacgtcg agaagccaaa cagaatgttg atgatgcccg tggcgataag
     4381 ccctatcccc ataaaataac gggcatttga gcggtcgctg acaatgccgg agacaaactt
     4441 cgacacgccg taggtaatgt aaaacagggt cgctaacagg ccgatatcgc tacggctgag
     4501 cacgccatta gcaaggattt ctggtacggc ggcgttaaaa cttttacgcg tgaagtaaaa
     4561 cagcgcgtaa cccagccaga tggtcagcag aatatgccga cgccagtagc gataacgggc
     4621 atcaatttca tgtttatcag tcattaatgg cgcatcggca ggcgctttca gaaacggcaa
     4681 catcatcaaa ccttagacat agcgttgagg tagagaaacg ctgacacgcg tgccgtgcag
     4741 acaggaaatg gtcaaggtgc cgcccagcgc cgttacgcgc tcgcgcattc cggtgaggcc
     4801 aaaaccttgt tgcccggaac ccggcggtaa accgctgcca tcgtcttcaa tcaccagcat
     4861 caaccgttca tcctgcagcc agccttgcag ggtgaccgcg ctggcatcag catgtttcac
     4921 aatgttattc agcccttcct ggcagacacg aaacagcgtc acgcgctggt tttcgcttaa
     4981 cgctgactct tcgattcgcc attcgagatg gctgacaatg ccgcgccctt ccagctccat
     5041 ttcccgcatc agtgagcgga tggcctgctc tagggtgaga tcatccaact ggcgcggacg
     5101 taaccgccct aacaaacggc gcaccgcgtc gtacacgccc agcgacagtt gttcgatgag
     5161 ctgcccgctc tgcttcacac tggcgttatc tgccgccagt cgctggacaa tgcccgcttg
     5221 agtacgaatg gcagtgatgg tctggccgat atcatcatgc agctcacgcg ccacatcacg
     5281 gcgcacgctc tcttcagttt ctaacaaacg ttcagcgaga tgctgattgc gcgccagttc
     5341 cttttgcagc gactggttaa gttcacgcaa ccgctggatg ccagcgccca gtaacaaccc
     5401 tgtcagactt tgcaccagca gcgagagcaa taaatccaca ggatgatcgc gccaggtttg
     5461 actggcgatc agcgcgatgg cgttcatcaa tgtcgctatc agcgcccctt gccagccgta
     5521 gtgccaggcc agcgcgataa tcggcagcgc caggcagaat ggcgtaaagc gcgacagttc
     5581 gtccggcaag cccaactgga gccagagact gataacaaac agcagcaagt accagaccag
     5641 atgccgcccg cgccagttga tcggttgaga aactaacgac ggcccgagcg gcagccaggt
     5701 gttattggcg agataatgcc agaacaccag acatatcggg gccagcgtca ggccgccagt
     5761 taaagttaac agcagcgcat tccacgactc tttgccgtgc caaagccagg gcagcgactg
     5821 caacaacgcc gccgccgtta acgccgcacc ctgtaacagc aaggtgcgcc agtcacgttg
     5881 atggcgatag cgcgagatca gcgccacggg cagtaacgtc agtaaactac cgatcatcaa
     5941 taaggagaaa tgggttaaac cgaccgcctg cgttagccag taaatcagca gccactccgc
     6001 tcccagcagc accggccagt atccgcgcgg gcattgcagc attagcccta gccgcagacc
     6061 aaacggaaat aacagcaccg ccatatcagg gcgctcaacc aggtgcaggc tgatactcca
     6121 caggcaaaac catgcggcag agaagataaa aaagcaggca ataacggtaa ttaagcggga
     6181 gaacaacgtc ttcatcacca gccatcaaac atacggcgcg ccagttcaac gtcgttactg
     6241 acgcccagtt tttccatcag attggcgcga tggacgtgta ccgttttcgg tgacaagccc
     6301 agttcggcgg caatctcttt caccgccatt ccttgcgcca gtttttccgc cacctgccgt
     6361 tcacgtttgg ttagtgggtc ctggcgaccg gatgccagtt taatggcaat atccggcgtc
     6421 agataacagc cgcctgtggc aaccgtatgc accgcagcaa tcagttcgtc agggctacaa
     6481 cgcttggaga gaaagccacg cgcccccgcg ttaagcgcct gctcaaccag cgccggactg
     6541 tcatgaacgg agagcataat tgtcgccata cctttcggca gctggcttag cagctccaga
     6601 ccggagatat cgggcatgga gatatcgcaa atacacacct gcacaccgcg ccccggcagc
     6661 cccgccagcg cctcgcgccc cgaaccaaac tcggcaacta cttgcaaatc aggttccagc
     6721 cccagcaact gcgcaaagcc ggagcggacg atgaggtgat cgtctataag ggcaacggtg
     6781 atcatgattg ggtcctggcg gataaaaaac gcgcatacct taacgataag cgcgatgttg
     6841 ttcaagttgg atatggggta tgtattggct taatgatgtt gagcttatcg tcaaaacgct
     6901 tacctgagag gtaagcgttt ggtgcgtgtt attgagaaaa agcgaattat ttgtaggttg
     6961 caatcgtaat attgcctgag aaatttctcc ttagattgcg gatatcatta tcccttgccc
     7021 aataatcatc tttgtgtcta taactctcat cggaatgtac atagggagaa tcatcttcag
     7081 cgtcgttatg gataatattt tcgcctttag tggtatagat ataattagca acatcaaagg
     7141 tgtcattcgc gttatttacg actgcgccta agacgcaaag ggtttgagtg aatccgtatt
     7201 ctgagaaatc gacatgctga tggtaatttc taccattcaa agcaacaaaa ctcggacaaa
     7261 catctattgt atcgccaaca gacgtagcgg cgcgacgcac atcaagtgca gcgttatcgt
     7321 tagcaacatg aaatgcttgt gaacaggcat aaaacaaatt tctaatcccg gtacgactgg
     7381 ataaacaaga aataattttt gctatttttt ctggctttat accatcctgt actaaagaat
     7441 ctctcaccag atc
//
LOCUS       DAZKIP010000179         7432 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZKIP010000179 DAZKIP010000000
VERSION     DAZKIP010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.181"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(182..667)
                     /gene="sixA"
                     /locus_tag="ACHOUK_004361"
     CDS             complement(182..667)
                     /gene="sixA"
                     /locus_tag="ACHOUK_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFP9899679.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGKGEFNWQMSPCNLKMAKA
                     I"
     gene            complement(870..3014)
                     /gene="fadJ"
                     /locus_tag="ACHOUK_004362"
     CDS             complement(870..3014)
                     /gene="fadJ"
                     /locus_tag="ACHOUK_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFP9899680.1"
                     /translation="MEMTSAFTLNVRLDNIAIITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGIP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVAHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3014..4324)
                     /gene="fadI"
                     /locus_tag="ACHOUK_004363"
     CDS             complement(3014..4324)
                     /gene="fadI"
                     /locus_tag="ACHOUK_004363"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFP9899681.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVVESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQLLAEDNNIRGNSSLADFAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     ADLTLIDMHEAFAAQTLANIQLLGSERFAREALGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4504..4788)
                     /locus_tag="ACHOUK_004364"
     CDS             complement(4504..4788)
                     /locus_tag="ACHOUK_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFP9899682.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5160..6500
                     /gene="fadL"
                     /locus_tag="ACHOUK_004365"
     CDS             5160..6500
                     /gene="fadL"
                     /locus_tag="ACHOUK_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFP9899683.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGKTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRVFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6865..>7432
                     /locus_tag="ACHOUK_004366"
     CDS             6865..>7432
                     /locus_tag="ACHOUK_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899684.1"
                     /translation="MLSSISINNTSAAYPESINENNNDEVNGLVQEFKSLFNGKEGIS
                     TCIKHLLELIKNAIRGNDDPDRININNSSVTYINIGSNDTDHITIGIDNQEPIKLPAS
                     YKDKELVRTIINDNIVEKTHDDINNAEFTDGGIDDRSDIGIDSNDTDDIDIDNQEPIK
                     SPANDENKKVGQTIINENIVENTHDINNK"
BASE COUNT         1958 a         1940 c         1705 g         1829 t
ORIGIN      
        1 gtaaaaatac attacaactt agtctcattt cgtcagaaat taatagggaa gcagaggaaa
       61 aagtgcgatg aattctgtat cgaagcgtat caactccgtg aagggttttg agtgaatttc
      121 tactattgca aaaagacaaa tgcccgatgt acagcttaca tcgggcattt tttcaaacga
      181 atcagatagc tttcgccatc ttcaaattac atgggctcat ctgccagttg aactcgcctt
      241 ttccgctttc atcaagcgtt acgctggcaa tggccgaggt ggtaaacata ggcggcgttt
      301 cgcccggaca taactcggcg accagatacc caactaacgg taagtgggag ataaccaaca
      361 ccgaagcaac accttcattg gtcaacgcct gcaaataggc actgaccaga ccaacatcgc
      421 cgcagggcgt taactccggc agaacttctg cactggaagg caggttcaga caatccccaa
      481 cttcctccag tgtttgctcg gctcgcagga acgggctcac cagaacgcgt tcgatttcca
      541 ctttttgacc tttcagccag ttcgccatca ggcgagattc gtcacaaccg ttagtggtca
      601 gaggacgaac cgaatcactg gcggcatcga gggctgcgtc gccgtgacgc atgataaaaa
      661 cttgcatatt gcaccgcttt tgttaaccag attcgcccgt tttctttcat ctgaaaccag
      721 agaaagaaaa cggtggccgg cattgtgcct tatccattca ccgaatgaaa cgctgttttt
      781 tacctcaatg gcgtaagtat agtcaatatg cgtttacatt ttgaccaaca atccgccatt
      841 cagcgcggat tcatatagct ttgaccttct tattgcaggt cagttgcagt tgttttccaa
      901 aaactttccc cacgcgcacc catctcgact aaacgctcgc aaggggtaaa acgtgaacca
      961 tactgcgtgg ccagtcgttg cattattgca accacttcgc ccgcgccgag agaatcgata
     1021 tagcgaaacg gtccaccgag aaatggcgga aaaccaatgc caaataccgc accaatatcc
     1081 ccgtcacgca cgctacggat aacctgctca tcaacacaac gtacagcttc attcagcatc
     1141 aacatcacac accgttcagc aacctgcggt gcggagagtc gcccctgccc ttgtgcgcca
     1201 atgagcgggt aaatggcagg atcgacctgt tttttgcttt tacgcccttt ctgaccataa
     1261 agatagaaac ctcgaccatt ttttctgcct ttgcgatcgt cgttcaaaat tgaagaaaca
     1321 acatttgcag gcgcgctaaa acgttctcca taagcggctt ccagtacagg aataatttta
     1381 gtcccggtgt cgattcctac ctcatccaaa agttggattg gacctaccgg aaaaccaaat
     1441 ttcactagcg cggcatcaat gtgcgctacc cgttcacctt cggtcagcat gcggatagct
     1501 tcattaatgt aaggcgctaa gatgcgattg acgtaaaacc cggctttgtc acgcacgaca
     1561 atgggcgttt tgccctgttt tttcgccagt tttaccgtag tggcgatggt ttgtgctgat
     1621 gtacccgcat gaggaataat ctccaccagc ggcatttttt ccaccggact gaagaaatgc
     1681 aggccgataa cttgctcagg tcgcgtggcg tgagcggcga tatcaccaat cggtaaagat
     1741 gacgtattcg aagcaaagat ggtatgagcg gcgcaatttt gctcaacttc cgccaccatc
     1801 tgttgtttca attcgagatt ttcaaacacc gcttcaataa tcagatcgcg atgggcaaag
     1861 ccgcgatagt ccgtcgttcc ggagattaat gccagctgtt tgtcacgttc gctggctttg
     1921 agatgacgac ggcgaacttt gccctccagc tgatcccaac tgtacttcag cgcatgattt
     1981 atgccctgcg ggttgatatc tttaattctg accggaatcc ccgctttaca agcagtgaca
     2041 taagcaatac cgccgcccat caagccacca cctaaaatcc ccacgctgtt taatggcgca
     2101 ggcggcgcat cactgccggg atctttcttc acttccgtac tggcaaaaaa gatactacgc
     2161 aaagcttgcg attgtggcgt catcgccagt tcgccaaacg cccgtgcttc agcgtcatag
     2221 ccactgctgg tgccctgcgc taatcccgtt tcaacaacct ccaggatgcg ttctgtcgcc
     2281 ggataattgc cttgagtttt gtgttctgtt ttcttgccga ccattttgaa cagcagcgca
     2341 cgacctaacg gccccgccag aatacgctcg cgtacaggta gagggcggga agatgggcga
     2401 tccttctttg ccagctcaac agcggcttcc agcagaatgg agtgcggaac aacatcatcc
     2461 accagcccca gctttaatgc ctgtttcgcc cgaagttgtt ttccggtgag gatcatctct
     2521 aatgctgtgc tgacgcctat cagacgcggt aaacgctggg tgccgcctga accgggtaac
     2581 aatccaagtt gtacttcagg caaaccgagc accgttttag gatcgtcagt acaaacgcga
     2641 ccgtggcacg ccagcgccaa ctccagccca ccacccaggc aagcgccatg aatagccgcg
     2701 ataaccggaa tgggcaaagc atgaatctcc gccatcaact gttggccctg ccgcgccaga
     2761 gcttccgctt cttgcgccgt tttgcaattg ccgatcatgt tgatgtctgc gccagcaatg
     2821 aagttgtccg gtttagcgga gacaaacacc acgcctcgca actctttgtt ttcacggagt
     2881 tgcttaataa tggcgcgcac ctgcgaggca aactccgcct tcagggtatt cattttctca
     2941 cccggtacgt cgatggtgat gatagcaatg ttgtccagac gaacattaag agtaaacgct
     3001 gatgtcattt ccattattcc gcctccagaa ccattgccgc gccaagccca ccggcagcac
     3061 aggcggtaac taaaccaaat ccaccgccgc gacggcgaag ttcatgcaat gtctgggtaa
     3121 tcatccgcgc gccggtcgcc gcgaagggat gcccgtaagc aatcgaaccg ccaagcacgt
     3181 taaatttgct atcgtccact tcgccagtgg catgtgcacg ccccagcgct tcacgagcaa
     3241 aacgttcact acccagtagc tgaatattgg ccaacgtctg ggcggcaaag gcttcgtgca
     3301 tatcaatcaa tgtcagatct gccattgtca aaccggcacg ctccagcgcc agcggtgttg
     3361 accaggctgg accgagcaac atgtcctgcc agacatcgat agcggtaaat gcgtagctgc
     3421 gcagatatcc cagcggtacc agccctaatt ctttcgcccg ggattcagtc atcaggatca
     3481 ccgccgctgc gccatcggtc agcggcgtac tgttcgccgc cgttaccgtt ccgtgtttac
     3541 gatcaaacgc cggacgcagc tttgcgaaat cagcaagcga ggaattaccg cgaatattgt
     3601 tgtcttccgc gagcagttgt ttataaggag ggataaaggc agtcatcacc tcttctttga
     3661 gttttccgtc tgaccatgcc tgagcggcac gctgatgcga acggtgcgct aatgcatctt
     3721 gctgttctcg ggtgatgccg taggttttcg ccatttgctc tgcagtgtcg cccatccgca
     3781 agccggtaga atattctgct accgcaggtg gtacgggcat taagtcgcgc aaacgcaggc
     3841 gagagaagag tttcagacgc tggctcattg tgcgggcttt gttgacatca accagcacgc
     3901 gcgccagttt tttactgacg ccaattggca ataccgaaga ggaatctgcc ccaccggcaa
     3961 tccccgctcg gatagtcccc gccatcaggc tttctacgac gtttgcaacc gcctggaaac
     4021 tggtagcgca agcgcggctg acgctgtaag catcggtatg cacattcatt ccagtaccga
     4081 gaacaatttc acgcgcaatg ttgggagctt caggcatttg tacgacctga ccaaagacca
     4141 gttgttcaat cacttcggcg gggatctcgc tgcgtgccag cagttcgcct accaccatct
     4201 tccctaaatc aaccgcggga atgccatgaa aagccgtcgc ctggcgggca aaaggcgtac
     4261 gtaaaccgct aacaatggcg atacgatcgc cctggcgggt aaccagcggt aaaacctgcc
     4321 ccataacact cccctgtaaa aaaataaata aagtggtctg acctgatcat agtcttaacc
     4381 atttttttac atttagccaa gcggagaaaa gggaaattgg gagctatgac acagtgaaga
     4441 caggagaaag aaaagaaaac gcccctacct gttgctggca ggagcgtact tatccgagag
     4501 gaattaacgc agacccagct ggaagatcag catttcagct tcgcaggcaa aagtgaaatc
     4561 gatatccagg cgcacaccat cagactcttc agtgaaagtc ggggagattt tgcacggttc
     4621 ggattccacg ctacgggctt tctcagtcag cgccgccagc gtttgttctg cttctgcgcg
     4681 gtttgcaaac acgcggctgt aagacgctgt gcagtcggag ttgtccataa tggtgccaac
     4741 atccatacag cagcaaaccg gggtttcatc agcactacat ttactcatcg ttgatttcct
     4801 ctgtatgtgc acccaaggtg ccagataaac gttgtggata ttttacgctt ccggaaagtg
     4861 ctgctccagt tgttaattct gcaaaatcgg ataagtgacc gaaatcacac ttaaaaatga
     4921 tctaaaacaa aattcacccg aatccatgag tgcgccacct ccaaattttg ccagctggat
     4981 cgcgtttctt agatcatatt tgaaaaaaga tagaaacata cttgcaacat tccagctggt
     5041 ccgacctata ctctcgccac tggtctgatt tctaagatgt acctcagacc ctacacttcg
     5101 cgctcctgtt acagcacgta acatagtttg tataaaaata aatcattgag gttatggtca
     5161 tgagccagaa aaccctgttt acaaagtctg ctctcgcagt cgcagtggca cttatctcca
     5221 cccaggcctg gtcggcaggc tttcagttaa acgaattttc ttcctctggc ctgggccggg
     5281 cttattcagg ggaaggcgca attgccgatg atgcaggtaa cgtcagccgt aaccccgcat
     5341 tgattaccat gtttgaccgc ccgacatttt ctgcgggtgc ggtttatatt gacccggatg
     5401 taaatatcag cggaacgtct ccatctggtc gtagcctgaa agccgataac atcgcgccta
     5461 cggcatgggt tccgaacatg cactttgttg caccgattaa cgaccaattt ggttggggcg
     5521 cttctattac ctctaactat ggcctggcaa cagagtttaa cgatacttat gcaggcggct
     5581 ctgtcggggg tacaaccgac cttgaaacca tgaacctgaa cttaagcggt gcgtatcgct
     5641 taaataatgc atggagcttt ggtcttggtt tcaacgccgt ctacgctcgc gcgaaaattg
     5701 aacgtttcgc aggcgatctg gggcagctgg ttgctggtca gattatgcaa tctcctgccg
     5761 ggaagactcc tcaagggcaa gcattggcag ctaccgccaa cggtatcgac agtaatacca
     5821 aaatcgctca tctgaacggc aaccagtggg gctttggatg gaacgccggt atcctgtatg
     5881 aactggataa aaataaccgc tatgcactga cctaccgttc tgaagtgaaa attgacttca
     5941 aaggtaacta cagcagcgat cttaatcgtg tgtttaataa ctacggtttg ccaattccta
     6001 ccgccacagg tggcgcaacg caatcgggtt atctgacgct gaacctgcct gaaatgtggg
     6061 aagtgtcggg ttataaccgt gttgatccgc agtgggcgat tcactatagc ctggcttaca
     6121 ccagctggag tcagttccag cagctgaaag cgacctcaac cagtggcgac acgctgttcc
     6181 agaaacatga aggctttaaa gatgcttacc gcatcgcgtt gggtaccact tattactacg
     6241 atgataactg gaccttccgt accggtatcg cctttgatga cagcccagtt ccggcacaga
     6301 atcgttctat ctccattccg gaccaggacc gtttctggct gagtgcaggt acgacttacg
     6361 cgtttaataa agatgcttca gtcgacgttg gtgtttctta tatgcacggt cagagcgtga
     6421 aaattaacga aggcccatac cagttcgagt ctgaaggtaa agcctggctg ttcggtacta
     6481 actttaacta cgcgttctga taacgcgttc gcctggataa agtcacctgc tatgcaggtg
     6541 actttaactc ccccacttca ccgaaagtag tttcctcatt gcttgcctcc tgagttttgc
     6601 aaacagcctg atggcagcta gccctttcaa atcagtaagt ggtatccaca gacaaacctg
     6661 aagggaaacg catattttca ggcgttctgc tcgtccttct caaagagttt acttttctga
     6721 atttccagga tactcaaccc cctggctatt gtgcgctcat acacgcaaat taaagatagg
     6781 ttctaaataa atgagcgttt tttgatagtc tatttcatta ggtaatatat atttgtaaca
     6841 aatcaatcaa catggaataa aatcatgcta tcatctattt caatcaacaa taccagcgca
     6901 gcttacccag aatccatcaa tgaaaataac aatgatgaag ttaatggatt agtacaagag
     6961 ttcaaaagcc tttttaatgg taaggaagga ataagcacct gtattaaaca tctacttgag
     7021 cttataaaaa acgccatacg aggaaacgac gatcctgata gaataaatat caataattct
     7081 tcagttactt atattaatat tggttccaat gatacagacc atattactat tggtatcgac
     7141 aaccaagaac caataaaatt acctgcgagt tataaagaca aagaactcgt ccgtactatc
     7201 attaatgaca acatagttga gaagactcat gatgatataa ataatgctga atttactgat
     7261 ggtggtattg atgatcgtag tgatattggc attgactcca atgatacaga cgatattgat
     7321 attgataatc aagaaccaat aaaatcaccc gcgaacgatg aaaacaaaaa agtcggccaa
     7381 accatcatca atgaaaacat agtagagaat actcatgata tcaataacaa ga
//
LOCUS       DAZKIP010000180         7392 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZKIP010000180 DAZKIP010000000
VERSION     DAZKIP010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.182"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..873
                     /locus_tag="ACHOUK_004367"
     CDS             <1..873
                     /locus_tag="ACHOUK_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFP9899685.1"
                     /translation="VDSDIARLLLKLRKSRNLTVTELAQRSGVSQAMISKVERGTTDA
                     GAGFEQTLTGMTVGIDSRNDIPEGIATLGAFMGYSHSHIGFDRGGHGSVDSYSLGGYA
                     SWEHESGFYLDGVVKLNRFESNVAGKMSSGGAANGSYHSNGLGGHIETGMRFTDGNWN
                     LTPYASLTGFTADNPEYHLSNGMESKSVDTRSIYRELGATLSYNMRLGNGMEVEPWLK
                     AAVRKEFVDDNRVKVNSDGNFVNDLSGRRGIYQAGIKASFSSTLSGHLGVGYSNGAGM
                     ESPWNAVAGVNWSF"
     gene            complement(1245..1511)
                     /gene="minE"
                     /locus_tag="ACHOUK_004368"
     CDS             complement(1245..1511)
                     /gene="minE"
                     /locus_tag="ACHOUK_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFP9899686.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(1515..2327)
                     /gene="minD"
                     /locus_tag="ACHOUK_004369"
     CDS             complement(1515..2327)
                     /gene="minD"
                     /locus_tag="ACHOUK_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFP9899687.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2351..3046)
                     /gene="minC"
                     /locus_tag="ACHOUK_004370"
     CDS             complement(2351..3046)
                     /gene="minC"
                     /locus_tag="ACHOUK_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFP9899688.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            3566..3934
                     /gene="ycgJ"
                     /locus_tag="ACHOUK_004371"
     CDS             3566..3934
                     /gene="ycgJ"
                     /locus_tag="ACHOUK_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFP9899689.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLSEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(4037..4438)
                     /gene="pliG"
                     /locus_tag="ACHOUK_004372"
     CDS             complement(4037..4438)
                     /gene="pliG"
                     /locus_tag="ACHOUK_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFP9899690.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            4509..4667
                     /locus_tag="ACHOUK_004373"
     CDS             4509..4667
                     /locus_tag="ACHOUK_004373"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899691.1"
                     /translation="MAFLRSVKSRRGIFAGGITYKGAWTQQTVEIYSWLACGAGFIDV
                     SLCQNLAS"
     gene            4679..4972
                     /locus_tag="ACHOUK_004374"
     CDS             4679..4972
                     /locus_tag="ACHOUK_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFP9899692.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            5044..5703
                     /gene="ycgM"
                     /locus_tag="ACHOUK_004375"
     CDS             5044..5703
                     /gene="ycgM"
                     /locus_tag="ACHOUK_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFP9899693.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            5795..6241
                     /gene="ycgN"
                     /locus_tag="ACHOUK_004376"
     CDS             5795..6241
                     /gene="ycgN"
                     /locus_tag="ACHOUK_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFP9899694.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(6448..7358)
                     /gene="hlyE"
                     /locus_tag="ACHOUK_004377"
                     /pseudo
     CDS             complement(6448..7358)
                     /gene="hlyE"
                     /locus_tag="ACHOUK_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309704.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
BASE COUNT         1901 a         1631 c         1781 g         2079 t
ORIGIN      
        1 gtcgatagcg atattgcacg tttgttactc aagctcagaa agtctcgaaa cttgactgtt
       61 acagagcttg cccaacgatc tggtgtcagt caggcgatga tcagcaaagt tgaacgaggc
      121 accactgatg cgggtgccgg gtttgagcag acgctgaccg gaatgacagt ggggatcgac
      181 agccgtaatg atattcctga ggggattgcg acgctgggcg cttttatggg ttattcccat
      241 tcacatatcg gttttgatcg tggaggacat ggcagtgtgg acagttattc tctgggcggc
      301 tatgccagtt gggaacatga aagtggtttc tatctggacg gtgtcgtgaa gctgaaccgt
      361 tttgaaagta acgtagccgg taaaatgagc agcggtggag ccgccaatgg cagttaccat
      421 agcaacgggc tgggcggtca cattgaaacc gggatgcgat ttaccgatgg taactggaac
      481 ctgacgccgt atgcctcgtt aacggggttc accgctgata accccgaata tcatttatcc
      541 aatggcatgg aatcgaaatc agtcgatacc cgcagtatat atcgtgaact gggtgcaacg
      601 ctgagttaca acatgcgtct ggggaacggt atggaagttg agccgtggct gaaggcggct
      661 gtgcgcaaag aatttgtcga tgataaccgg gtgaaagtga atagtgacgg taatttcgtc
      721 aatgatttgt cgggcagacg tggaatatac caggcaggta ttaaagcctc attcagcagt
      781 acgttaagcg ggcatcttgg ggtggggtat agcaacggtg ctggtatgga atccccgtgg
      841 aacgcggtgg ctggtgtgaa ctggtcgttc tgaccatcaa cgattaaact gcgcttcggc
      901 gcagttttcg tttacaggat gttaaaagag aaaattctgg ggtaaaaaaa gcccgccagt
      961 tacggcggga aacctcatcc tatgggagaa caatgaataa tgaaattgcg gggttatcat
     1021 ctcccagtat atcaacacta acaataaggt tatttactca accaggcata aatattttgt
     1081 tttgtgcgtg ggaacagcct taaggtgtaa agggggaggt ggaaatagca atgaggagta
     1141 tcagcaagaa tactcgccgc tttaccacaa cgtggatgag agggatgaaa aactcaaggc
     1201 agagataact ctgccttgaa gataaatgcg cttttacagc gggattattt cagctcttct
     1261 gcttccggta aggtcacgtt cagctcaaga atagaaatat cgccatcttt ttgctcaagc
     1321 tgtacggtta ccatctcagg atcaatttgt acgtatttac aaatgacctc aagaatatct
     1381 ttacgcaact gcggcagata atgcggttct gcatcgctgc gacggcgttc agcaacaata
     1441 atctgcagcc gttcttttgc aatgttggct gtatttttct tccgcgagag aaagaaatcg
     1501 agtaatgcca taacttatcc tccgaacaag cgtttgagga agcctttctt ctcttcttca
     1561 atgaagcgga aaggacgttc ttctcccaac agacgttcta cggtatctgc gtaggcttta
     1621 cccgcatcgg cgttaatgtc gagaatgacc ggttcaccct ggttagaggc gcgcaatact
     1681 gattgatcct ctgggatcac gccgacgagt ttgatgcgca ggatctccag cacatcttcc
     1741 atgctcagca tgtcacctct gcttacgcgg cctgggttat agcgcgttaa cagcaggtgc
     1801 tctttaatag gctcttcgcc attttctgcg cggcgtgatt ttgacgccag aatgcctaaa
     1861 atacggtcag agtcgcgtac tgaggagact tccgggttgg tggtaataat ggcttcgtct
     1921 gcaaaataga gtgccattaa cgcaccggtt tcaatccctg ccggggagtc acaaacgata
     1981 aattcaaaat ccatcgcttt cagatcatca agaactttag cgaccccttc acgggtgagg
     2041 gcatctttat cgcgtgtttg cgatgccggc agaatataga gattttcagt acgcttatct
     2101 ttaattaacg cctgatttag cgttgcatcg ccctgaatga cgttgacgaa atcgtaaacg
     2161 acccggcgtt cacaacccat aatcaggtcg agattacgca ggccgatatc aaaatctatc
     2221 acgacagttt tctttccctt ctgggccaaa ccagtggcga tggccgcgct ggaggttgtc
     2281 ttaccaacac cccctttgcc cgaagtaaca acaataatgc gtgccataga aattccttgt
     2341 taaaaaggga tcaatttaac ggttgaacgg tcaaagcgtt ttcgactaac tgcagtcgcg
     2401 ccgctttgcc ataaaattct gctgggattt gatcactcag ccagtattca cctgcgatgg
     2461 acaccaattc cgccatcagg ttcgtacaaa atatttgcgt ttcccggtca ccacttgccc
     2521 ctgccagcgc acgaccgcgc atcatgccat agacatgaat gttcccatcg gcaatcaatt
     2581 cggccccagc gctaacgtgg cttgtaacaa tcagatcaca ttgtggagca taaatacgct
     2641 gaccggaacg caccggggta tctattaaac gcgtttttgt gaccggcgtt gtattttgcg
     2701 ctggagcctg cggtgcggga gctggacgtg gcgctttttc ctttccttcc gtcaggatag
     2761 gcagccccat tttttcaatt tcggctttaa gttgcgcatc tttgcagcca cttacgccaa
     2821 taacccgcaa accggttgcc gaaaccgcct tatgcatcgc tgaccagttt accgggtctt
     2881 ccagtgcact gacgttgagt acaacggggg catgttttaa aaatgcgggg gcctgagcga
     2941 ttttgtcttc cagcgcctga tggataacct taggttctgc ctcatgcaga tgaaccacag
     3001 ataaagtgaa gctactgcct ttaagctcga ttggcgtgtt tgacatcctg gccttactca
     3061 attagctatt aatcatcgcc agcgcgcgat gatgttccga agactataag gcatgttata
     3121 gtctggatta tattgaggca agtcaccctc ccatttattc agagtaaaag tctattctgt
     3181 gataaatggc gctgattcat agcttaaaaa atacccttgt caatcaaccc attgccgtcg
     3241 tacttttgat tcttcttatt tacgcttcct ttttccgcac cctaatcgtg acagcatctc
     3301 cagacctgtt taaatatcca gtattgaggc aagtgtattt tttcaccacg ccaggctttc
     3361 ttaactttcc attttttgca gcatcattaa agaacagata cccgttatct taatatccgc
     3421 cacagaattg ctctatcata gaaaccgcag aagacgtctg atacttctgt caggtcatcg
     3481 taactcgtcc aggtcagcgg ttttgattac ggattgatga tgaaatgagc ataagtgttg
     3541 tacggttgga ttatcaggat gtgctatgaa tatgatgcgt attttttata tcggattgtc
     3601 aggtgtgggg atgatgttct catcaatggc gtctggacat gacgctggcg gacttcaatc
     3661 tccggcgtgc ggcgttgttt gcgacccgta tatttgtgtg aactcagatg gcatttcccc
     3721 agagttaaca aggaaatatc tcagcgaaaa agccgctgaa aacttacaat cattacaagg
     3781 ctacgatccc agcgaattta cattcgctaa cggtgtattt tgcgatgtta aagaaaaatt
     3841 atgccgtgat gatcgttatt ttggtgtaga tggtaagcgg agtggaaaaa tcaatcaaac
     3901 caccacaaag atgttattta tgtgtcgtga gtgatgtctt gtttacatta tgttctattc
     3961 gtcattaacc tcttgagcga cagagaggag tggggcgtca acgtttatga cggggagact
     4021 ccccgtcggt tgacatttac ttaatttgaa tatcgacgtt atatttttta gttttgtttt
     4081 tacgggcgtc gtttcgggtt tgcagcactc gcagctcata tttgccagaa gcagggagtg
     4141 agtattgtcc gtgactatcg agttcagggg aatatctgga taaatcaaca gaatcatcga
     4201 ttcctggccc gaagaggtaa gtatcagcgc cttcattaga gatactcaca tgcactttct
     4261 gacctttttt ggcatagaag gtatatgtat cgtaatcgta tccctttatt tcgccggagt
     4321 actgagcgct gctatggcct tttctgaact caacattgac attcttaccc gcagcaaagc
     4381 tcgtggaggt tagcagtgca agcagcaata cagccttact gatgctcttg attttcattg
     4441 gtagtcttct gttcagaatg agtacgatga actgttataa tataacaatc ccttacggga
     4501 gaattccagt ggctttcctg cgttcggtaa aatcccgtcg cggcattttt gccgggggga
     4561 ttacttataa aggggcatgg actcaacaaa ccgtagaaat ttacagctgg ctggcttgtg
     4621 gcgcgggttt cattgatgtt agcttatgcc aaaacctggc atcttaaaga gtaaaagtat
     4681 gttttgtgtg atttatcgaa gcagcaagcg tgaccagacc tatttatatg tcgaaaaaaa
     4741 agacgatttt tcgcgtgttc ctgaggaact gatgaaaggt tttggtcagc ctcagttagc
     4801 gatgattctg ccgctggatg ggcggaagaa actggtcaat gccgatattg aaaaggttaa
     4861 acaggcatta accgagcaag gttactattt gcagttaccg ccaccacccg aagatttgct
     4921 gaagcaacat ctttccgtca tggggcagaa aacagacgac actaacaaat aaccgatatc
     4981 cggcggtggc attatctttg tcggcgcggg ttttcatatc cacgataagg tgaggggaac
     5041 gttatgtatc aacatcacaa ctggcaaggt gcgctgctgg attatccggt gagtaaagta
     5101 gtttgtgttg gcagtaacta tgccaaacat attaaagaga tgggcagcgc agtgcccgaa
     5161 gagccagtgc tgtttattaa accagaaacg gcactgtgcg atctgcggca gccattggcg
     5221 atcccatccg atttcggttc agttcatcat gaagtcgaac tagcggtgtt gattggcgcg
     5281 acgctgcgtc aggctacgga agagcatgtc cgcaaagcca ttgccggtta cggcgtggcg
     5341 ctcgatctga cgttgcgtga tgttcaggga aaaatgaaga aagccgggca gccgtgggaa
     5401 aaggctaaag cgtttgataa ctcttgtccg ctttccgggt ttattcccgc ggcggaattc
     5461 accggcgatc cgcaaaatac aacgctgggc ctgagcgtaa acggcgaaca acgccagcaa
     5521 ggtacgactg cggacatgat ccataaaatc gttccgctga tcgcttatat gagcaagttt
     5581 tttaccctca aggccggtga cgttgtgctg acaggcacgc ctgatggcgt cggcccgttg
     5641 caaagcggtg atgagctgac agtcactttc gatgggcatt ctttgacaac tcgcgttttg
     5701 taatactttt tgccgcctga aagcggcggc aaaacttgca tcgctgtgcc agactggtta
     5761 taaggtgcgt tttaacgtaa tggcggaaca cctgatgagc gatgtacctt tctggcaaag
     5821 taaaaccctg gacgaaatga gcgatgcgga atgggagtcg ttgtgtgatg gttgcggtca
     5881 gtgttgcctg cataaactga tggatgaaga caccgacgaa atctacttca ctaacgtcgc
     5941 ctgtcgccag ctcaatatta aaacctgtca atgtcggaac tacgaacgtc gtttcgagtt
     6001 tgaacccgac tgcattaaat taacccgtga aaatctgcca acattcgaat ggctaccaat
     6061 gacctgcgct tatcgtttgc tggcggaagg taaagattta cctgcgtggc atccgctgct
     6121 tactggttcg aaagcggcaa tgcatggtga acgtatctct gtgcgtcata tcgcagtgaa
     6181 agaatcagaa gtgattgact ggcaggatca tatcttaaat aaacctgact gggcgcagtg
     6241 ataaatttaa tagatgttga tgggggcttt cttaatatac gtctggatgc tcgccatttc
     6301 gtagccctgt ttttgaaagc gctctgtcat aatgagagtt acccgatacc ttattatcaa
     6361 caggtaactc tcaatcttta aaaaacgtct gtatttttaa atgtgataaa ctttatacct
     6421 tgagtacatg gagagaataa ttgctgatca gacttcaggt acctcaaaga gtgtcttctt
     6481 accgtgtctt ttctgatact cattacaggt gttaatcatt ttattggccg cttcttttag
     6541 caaagaaagc attaaatcat cataatcaac atagaatctg gttgtttcag tttccgtttt
     6601 tatctccccg atggcgctat ttcggtggtt aatttcaatt tggcggcatc gatatcttta
     6661 ttcgcttgtt taaccgtgtt agacagggtg gtaaagaaac tctgcacaga ttttaacttg
     6721 ttcttcaatt ctggaatcag tttcccttca actacgcccg cagcaataga ataggaaatg
     6781 attaaaccaa atggaccggc gacgacaccg gctgcggcac cggcatacgc ttccttcctg
     6841 attttatcta cctgtgactg gaaatagctg cttttttctg aaaaatcatt ggttaactgg
     6901 ctatctaacg ccagcagttt cccggaagcg ttgttgaaac tttgtgagct taccagcagg
     6961 gatttttgcg cttcattcag cttcgtgatg ccgtcatcca gtaccttaat gagaatgtct
     7021 ttctgggcgg atgctttctt ctcattatac tcatcaaata gcaaaatata cgctgcgagc
     7081 aattgcgtcg caacaccaca ccattcatac actgtttggg ttgcttcaaa atacttatcc
     7141 tggctatcca ttagtaaggt tttaatattg ccaactaaaa cggaggctgc ctgtgaatac
     7201 tcctgtttaa agcgacttaa ctcttttatg gtttcatcaa aggtctgcca ggggatgacc
     7261 tgatcgagat atttattata aagatccaat gctccatctg cggtttcgat tgcgttttta
     7321 actacctcta ccgttttatc tgcaacgatt tcagtcataa tcattcgcct ctttaaatat
     7381 ataaattgta at
//
LOCUS       DAZKIP010000181         7377 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZKIP010000181 DAZKIP010000000
VERSION     DAZKIP010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.183"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..552)
                     /locus_tag="ACHOUK_004378"
     CDS             complement(<1..552)
                     /locus_tag="ACHOUK_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFP9899695.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKNI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQR"
     gene            complement(565..2346)
                     /gene="gcl"
                     /locus_tag="ACHOUK_004379"
     CDS             complement(565..2346)
                     /gene="gcl"
                     /locus_tag="ACHOUK_004379"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFP9899696.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(2436..3251)
                     /gene="allR"
                     /locus_tag="ACHOUK_004380"
     CDS             complement(2436..3251)
                     /gene="allR"
                     /locus_tag="ACHOUK_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFP9899697.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(3329..3811)
                     /gene="allA"
                     /locus_tag="ACHOUK_004381"
     CDS             complement(3329..3811)
                     /gene="allA"
                     /locus_tag="ACHOUK_004381"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFP9899698.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            4041..4967
                     /gene="allS"
                     /locus_tag="ACHOUK_004382"
     CDS             4041..4967
                     /gene="allS"
                     /locus_tag="ACHOUK_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFP9899699.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            5036..6130
                     /gene="mnmH"
                     /locus_tag="ACHOUK_004383"
     CDS             5036..6130
                     /gene="mnmH"
                     /locus_tag="ACHOUK_004383"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFP9899700.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLHAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAQ"
     gene            6246..6611
                     /gene="ylbG"
                     /locus_tag="ACHOUK_004384"
                     /pseudo
     CDS             6246..6611
                     /gene="ylbG"
                     /locus_tag="ACHOUK_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(7173..>7377)
                     /locus_tag="ACHOUK_004385"
     CDS             complement(7173..>7377)
                     /locus_tag="ACHOUK_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899701.1"
                     /translation="SYFSLTNDDGGRFKKTLTVRAREQVAKIVSKNDPDTKKVWCKYG
                     KIPGQGDGVNLFLLVKLMLRIIL"
BASE COUNT         1888 a         1923 c         1765 g         1801 t
ORIGIN      
        1 ccgctgcatg tgataaatgt catactgaat tttcaaattg cagcaaccaa catcatcaat
       61 caatttcagc gcctgccgag ttccggtgag atggaaacca ggaatatcaa aatggttaat
      121 aggttcaatc agtaataaaa tatcttcttt catcagcata ttcgcggcat aacgcaggtt
      181 ttctacaagc gttgcgtgaa tctgttcact gctgaaacca gccggcgttt taccgaccag
      241 acagttaata tttttattac ccagcgcacg ggcataacga atcgctgctg ctacgccatc
      301 ccgaaactct tcttcacggc caggaataca ggcaataccg cgctcccccg ccgcccagtc
      361 acccgccggt aaattgtgca gcgtatgttc gagtttatta ctcgccagca cctgttttaa
      421 ttcttcaatg tcgtagtcat aaggaaacat aaattcaacg ccgcgaaaac cacactgcgc
      481 agctttctca aaacgggcga gaaaatcata ttcaccaaat aacatcgata aattagcaga
      541 gaaacgtaac attatttatc tcccttattc atagtgcatg aagcaggttt cagtcggtgc
      601 atccgctgcg ttatcggcga tatcttcaaa ttccatgacg ttatccagtt cgctgcccat
      661 cgaaatattt gtcacacgct cgagaataac ttccacgact accggtaccc gatattgcgc
      721 cattaaggct ttcgcctgtt caaaggccgg tgcaatatct tccggtttga agacgcgaat
      781 agctttacaa cctaaacctt ccgctacttt tacgtggtcg acgccgtaac cgttcacttc
      841 gctggagttg atattctcga aagcgagttg cacgcagtag tccatgtcaa aggcgcgttg
      901 tgactgacga atcaggccga gataggcgtt attcaccagc acatggatgt acggaatgtt
      961 gaactgcgcg ccaacggcta actcttcaat caggaactgg aagtcgaagt caccggaaat
     1021 cgccaccaca ttgcgtttcg gatcagcggc acaaaccccc agcgccgccg gaatcgtcca
     1081 gcctaacgga ccagcctgac cacagttgat ccagtggcgg tctttaaaga catgcagcat
     1141 ttgtgccgca gcgatttgtg acaggccaat ggtggtgaca taacaaacat cgcgaccaaa
     1201 ggctttgttc atctcttcat acacgcgctg cggtttcacc ggcacgttgt cgaaatgggt
     1261 tttgcgcagc aaagtgcgtt tgcgctgctg gcaatcggcg acccattctt tacgacacgg
     1321 cagacgaccc gctttttgca tctcctgcgc cacttcaacc agcagtgtca gcgccgcttt
     1381 agcatcagag acaatgccga gatccggaca cagcacgcgg ccaatttgcg tcggctcaat
     1441 atcgatatga acgattttgc gcccttcggt gtatttctct accgaaccgg tatgacggtt
     1501 agcaaaacgg ttaccgatac caaacaccat gtcggacgcc agcaacgttg cgttaccgta
     1561 acgatgcgcg gtttgcagac cgaccatccc ggccatcagt tcatgatcgt ccgggataca
     1621 gccccagccc atcagcgttg ggatcaccgg aacgctggtc agttcagcaa actgttgtaa
     1681 cagtgcagct gcgtcagcat tgattacccc gcccccggca acaatcaccg gacgttcggc
     1741 ctggattaac atttctacag ctttttcgat ctgcatacgg ctggcagcag gtttgtagac
     1801 cggcagcggt tcgtacatgt caggatcaaa ctcgatttcc gcaacctgaa cgtcgaacgg
     1861 taaatccacc agtaccggac ccggacgacc agaacgcatc aggtgaaatg cctgttgcag
     1921 cacgcgaggc accagcgccg cttcacgaac tgtaaccgcc attttgctga ccggtttagc
     1981 aattgcttca atatctacgg cctgaaaatc ttctttatgc agacgggcgc gcggtgcctg
     2041 gccggtaatg cacagaatag gaatggaatc agcagaagcg gaatagagcg cggtgatcat
     2101 gtccgtgccc gcaggaccgg aagtccccag acatacgccg atattccctg ccgttgcgcg
     2161 ggtataacct tccgccatgt gcgaagcacc ttccacatga cgcgccagaa tgtgacgaat
     2221 accgccgtgc ttacgcatcg ctgagtagaa cggattgatt gcagctcccg gaacgccgaa
     2281 ggcggtggtg ataccttctt tctccagcac atacattgcc gcgtcaacgg ctctcatttt
     2341 tgccatttta ttcctacctc tattaattgg aaaaattttc caactttcaa attttgtcaa
     2401 aaaaacccgc tccgcagagc gggatgcgac agacactatg gatgtgcttt cagtcccaac
     2461 gccgtgctga tatcgcgggc ggtgtctctg accagctcac cctgactgac aaaacgatct
     2521 tctgtcagtc ttgatgaagg cccggagatg gagatagcgg caacaacgct acctacatca
     2581 tcgtaaattg ctgaagctat gcaattcaga cctacaacat gctcttcttt atctacggta
     2641 tagcccagtt cacgcgcttg ttccaggtcc ttcagcaggg tgggcatatc cacaagcgta
     2701 gttggcgtaa actgctgcaa accggtttgc agaatgatgc tcatcaactc ctcttccgcc
     2761 agcggataaa gcagcgcttt gcccgcaccg gaagcatgca gtggcagacg actgcccagt
     2821 ggcgcgcaca tcctgaccat cgatttacac tctaactgac caattaatac cgcttcattg
     2881 ccgttacgga tcgcgacatt gaccgtttcg ccggaaagta acatcaggcg gcgcataaac
     2941 ggcccggcga cggagaggac atcgcggtta tggatgtacg ccgcaccgac gttaaagaca
     3001 cctaatccta tatgccacca gcctaactga ctgtcctgat agacaaaatc cgctgcctgt
     3061 aaaaccttca gcaagcgaaa ggtcgtggag agcggcaaat ccagattgag agaaatatcg
     3121 ctaaccgacg aacttccccc acttttttcc agatattgca gaatcgcaat tccccgctct
     3181 aatgcctgtg cgcccttctg tgccacaggc tccgcctgtc ctggcctgcc gcgccgtcta
     3241 acttccgtca tacctacgtt aactccgacc tacaagtgag cataaacaat gaacaattag
     3301 tttatcttat gcaagtcggt tgcaggcgtc acgcaaaaca gagttcctgt tcaggaatac
     3361 tttcaacatc acagttgtca ctgccgccgc gatcgatggt cagaaaatcg gtgacgcgct
     3421 gccaggcgaa aagtgggtga tgccagacgt tacgatggta attcactccc tgttcgccgt
     3481 tggtaataaa cgcccgcagg gttgacaggt ctggtttgtc gtcacctaac gctacgacca
     3541 ccacaaacac ctcacctttc atcgggataa aggcctgagt acccagcgga tgacgttcga
     3601 gttcgtgaat ggtcagcggc agattcgccg gttgcgctcg gttaatgctg ataagcgtac
     3661 ggtcttgctc aagaatctca accagcgcca aatcgtggta acgctccacc aggccattgt
     3721 taatatggaa aaaatcccgt tgctgcgttt cgattacgtc gccataagca ctaaaggctt
     3781 cctgacttaa cggtaatacc tgaagtttca tctttttgtc cttgtaacat aattccgttc
     3841 aaccagcgcc gaccggtcag cttggtttca ataacaggca atctaaaacc ttaagagaac
     3901 aacatcaaca caaaacttgg aatatttttc caaaactaga tttagtccac aaattgaaca
     3961 gggttatcat gtaaaaatac tgataaatca gaggataaga aaatcacgca acttgcggat
     4021 ttaaaagggg atacctgaag atgttcgatc cagaaacctt gcggactttc attgcggttg
     4081 ctgaaacagg aagtttttca aaagcggcag aacgattatg taaaaccacg gcgacgatca
     4141 gttatcgcat taaacttctg gaagagaata ccggagtggc gctgtttttt cgcacgactc
     4201 gtagcgtaac gttgacagcg gctggcgagc atctactttc ccaggccaga gactggctga
     4261 gctggctgga aagtatgccc agcgagctgc aacaggtgaa tgatggcgtg gaacgccagg
     4321 tgaatattgt catcaacaac ctgctctaca acccccaggc cgtcgcccag ttgctggcgt
     4381 ggctgaatga acgttacccc tttacccagt ttcacatctc ccgacaaatc tatatgggcg
     4441 tctgggactc gctattgtac gaaggttttt cactggctat cggcgtcacg ggaaccgaag
     4501 cgctggcaaa tacctttagt cttgatccct taggatcggt gcaatggcga tttgtcatgg
     4561 cggcggatca tccgctggcg aacgttgaag agccgctaac agaagcgcag ttgcggcgct
     4621 ttccggcggt caatattgaa gacagcgccc gcaccttaac caaacgcgtc gcctggcgat
     4681 tgccagggca aaaagagatt attgttcctg atatggaaac gaaaatcgcc gcccatctgg
     4741 cgggcgttgg cattggtttt ttgccaaaat cgctttgcca gtcaatgatc gataatcaac
     4801 aactggtcag ccgggtaatc ccaacgatgc gccctccttc gccattgagt ctggcatggc
     4861 gcaaatttgg cagcggcaaa gcggtagaag atattgtgac cttgtttacc cagcgcaggc
     4921 cggaaatcag cggattttta gaaattttcg gcaacccacg cagttaaaaa tcaagtttcc
     4981 ttgcgcgttg gcgcgtaaaa tagcgcgcaa gctgatttga ggttcacttt taactatgca
     5041 agagagacac acggaacagg actatcgtgc cctgctgatt gctgatacgc ccattattga
     5101 tgttcgcgcc cctatcgagt ttgagcaagg cgcaatgccc gccgctatca atctgccgtt
     5161 aatgaataac gatgaacgcg ccgccgttgg cacctgctat aaacagcaag gctcagacgc
     5221 agcgctggcg ctgggacata aactggttgc gggtgaaatt cgtcagcagc gcatggacgc
     5281 ctggcgggca gcctgcctgc aaaatccgca aggtattctc tgctgcgccc gtggcggtca
     5341 gcgctcacat attgtgcaaa gctggttgca tgcagcgggg attgattatc cgctggtgga
     5401 aggcggttat aaggcactgc gccagaccgc gattcaggcg actattgaac tggcacaaaa
     5461 accgatagtg ctgattggcg gttgtaccgg cagcggtaaa acgctgttag tgcagcaaca
     5521 gccgaacggt gttgatctgg aagggttggc gcgtcatcgc ggttcggcgt ttggtcgcac
     5581 gttacaacca caacttagcc aggcgagttt tgaaaacctg ctggctgccg aaatgctaaa
     5641 aaccgacgcc cgtcagaatt tgcgcctgtg ggtgctggaa gacgaaagcc ggatgatcgg
     5701 ttcgaatcac ctgccggaat gcctgcgcga gcgaatgact caggcggcga ttgcggtggt
     5761 agaagatccg tttgagatcc gtcttgagcg cctgaacgaa gagtatttct tgcgtatgca
     5821 tcatgatttt acccacgcgt acggcgacga acagggctgg caggaatatt gcgaatacct
     5881 gcatcacgga ctttcggcta ttaagcgtcg gctggggcta cagcgatata acgaactggc
     5941 tgcaaggctg gatgcagcac tgacaacgca actcaccacc ggcagcaccg acggtcatct
     6001 ggcctggctg gtgccgttac ttgaagaata ttacgacccg atgtatcgct atcagcttga
     6061 gaaaaaagcg gaaaaagttg tctttcgcgg tgagtgggcg gaagtggcgg aatgggttaa
     6121 ggcgcagtaa tgagggacgt atttgtatta gtcgcgactt gatctgacac atggccttga
     6181 aaggttgaga gttaccggtt ttgatatgag tgtcgaatcc ttatccaaaa caagaggtaa
     6241 ctctcatgct tcatactgcc aatcccgtca tcaaacacaa agccggtttg ctcaatctgg
     6301 ctgaagaact cagcaacgta tcaaaagcct gtaaaatcat gggcgtttcg cgtgatacgt
     6361 tttatcgtta ccgcgaactg gtcgctgaag gcggtgtgga tgcgcagatt aatcgtagtc
     6421 gccgcgctcc taaccttaag aaccgtaccg atgaggcaac tgaacaggct gttgttgatt
     6481 acgccgtcgc tttcccgaca catggtcagc accgggccag caacgaactg cgtaaacagg
     6541 gggtttttat ctccgacagt ggtgtccgtt ctgtctggtt actccataac cttgagaatc
     6601 tcaaaagacg atactgaaca aattttatca ggtgacgttc cgtaaaaagt tgtatggagt
     6661 tctcgatgca gtcggcgcga ctatcagtac ccccgctgaa ccctggacta tcgggccgcc
     6721 gtactggggc atgtcaccct ggcgtgccgc tggttttccg ctaatggttt atttcctctt
     6781 tctttcccgg caagaattta ttttaatccg gaaggattat ttattttcag atagggactc
     6841 acattaaaca cgtaacattt taattaatgc atggctgaat atgtattaca caatgtcaga
     6901 tatcgtaaaa gaccagtgca attctatgta aactctctga ctgttcattt tatttgttgt
     6961 ttcagggtcg gaactcaccc tcaataaaca actgaatacg agtagatatt catcgtctga
     7021 gctatatggc tttacacaat agccgacatg tcttttctca gtcaggaatt ttacttttcc
     7081 tcactacgaa gttgacgact tcgatatggg atagactctt aattcaagca attactctgc
     7141 acaagcatca ggcaatccag ctccaatatt tgttataaaa taatgcgtaa cattaatttc
     7201 accaacaaaa aaaggtttac accatcccct tgccctggta tcttaccata tttacaccac
     7261 actttttttg tatctggatc attctttgat acgattttcg ccacttgttc cctggccctc
     7321 actgtcaatg ttttcttaaa cctcccccca tcatcatttg ttagggaaaa ataagaa
//
LOCUS       DAZKIP010000182         7322 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZKIP010000182 DAZKIP010000000
VERSION     DAZKIP010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.184"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(678..1484)
                     /locus_tag="ACHOUK_004386"
     CDS             complement(678..1484)
                     /locus_tag="ACHOUK_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFP9899702.1"
                     /translation="MSGSVIHSQSAVMVPAVYSAGQPASLPVAIDYPAALALRQMSMV
                     HDELPKYLLAPEVSALLHYVPNLHRKMLLATLWNTGARINEALALTRGDFSLAPPYPF
                     VQLATLKQRTEKAARTAGRMPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNRR
                     TGRTEKARIWEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIP
                     LKVLQSLMGHKSISSKEVYTKVFALDVAARHRVQFAMPESDAVAMLKQLS"
     gene            complement(1485..1790)
                     /gene="ccdB"
                     /locus_tag="ACHOUK_004387"
     CDS             complement(1485..1790)
                     /gene="ccdB"
                     /locus_tag="ACHOUK_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HFP9899703.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1792..2010)
                     /gene="ccdA"
                     /locus_tag="ACHOUK_004388"
     CDS             complement(1792..2010)
                     /gene="ccdA"
                     /locus_tag="ACHOUK_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HFP9899704.1"
                     /translation="MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERW
                     KAENQEGMAEVARFIEMNGSFADENRDW"
     gene            2553..3152
                     /locus_tag="ACHOUK_004389"
     CDS             2553..3152
                     /locus_tag="ACHOUK_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001495930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP9899705.1"
                     /translation="MTSLSPDTVRRIEDAAAALIAAGTPNPTNEQVRQHLGGGSLSHI
                     SPVMRAFRARQREQATPLPPELAQLLTGQLGLLWQAAVKQAEAGALAAREQADDDIAR
                     ADKERDEALANVAALESELAVLREVVAERDRLLQEVRELRAEALPLREQVARLTATGE
                     HLAAQLQDTKAELKEAREDGRQLQTELLALARQDGKVKK"
     gene            3149..3769
                     /locus_tag="ACHOUK_004390"
     CDS             3149..3769
                     /locus_tag="ACHOUK_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899706.1"
                     /translation="MRQERVLRIKAFRRALELGGGERADGCRHFPRWKTELRNFPHSS
                     CDLASNTLAYYLKERERCHPCIIFMEGNAGFHEAENSTVHGHVIVLLDGEYIDLTLDQ
                     FDEYPDYIPYEPVESDGQIGKLLRNIMKHEDPVKTRQIDLDGGEELYAWLRDTADEVL
                     AADPEWQARERSIEEAREAAITVFPFLSDVQKTECEQGPDSQEAAR"
     gene            3766..3966
                     /locus_tag="ACHOUK_004391"
     CDS             3766..3966
                     /locus_tag="ACHOUK_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899707.1"
                     /translation="MKIYHYTDLNGLKGIIESGSLWATHFSFLNDSNELTHGMNCLEN
                     ALQYLRDDFNPKTLKFIEQAPL"
     gene            4374..5351
                     /locus_tag="ACHOUK_004392"
     CDS             4374..5351
                     /locus_tag="ACHOUK_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFP9899708.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PVSLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKLFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(5636..6376)
                     /locus_tag="ACHOUK_004393"
     CDS             complement(5636..6376)
                     /locus_tag="ACHOUK_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9899709.1"
                     /translation="MNNVIPLQNSPERVFLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGARPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLR"
     gene            complement(6497..6727)
                     /locus_tag="ACHOUK_004394"
     CDS             complement(6497..6727)
                     /locus_tag="ACHOUK_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HFP9899710.1"
                     /translation="MWILNVFQVHKSVRIYIAHFSVFNCHLHPSIDRSLASWHYPNSP
                     KNGKPALSPRHDWLNTGSLLQNVPLRIWFYPQ"
     gene            complement(6896..7180)
                     /locus_tag="ACHOUK_004395"
     CDS             complement(6896..7180)
                     /locus_tag="ACHOUK_004395"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFP9899711.1"
                     /translation="MLLALLSSTDNFCLSSTELSERLDVSRTYITRACDSLEKFGFIK
                     RMESKEDRRSKNIYLTSDGNLYLQRTTRIYGRYLKKYGPTLQMMKSKHLK"
     gene            complement(7210..>7322)
                     /locus_tag="ACHOUK_004396"
     CDS             complement(7210..>7322)
                     /locus_tag="ACHOUK_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9899712.1"
                     /translation="IIYQQQNEQLYPAQEHLLLQLCMRVTKKLTDILMPP"
BASE COUNT         1864 a         1874 c         1820 g         1764 t
ORIGIN      
        1 acaatttttt agattgtcac actaaataaa aaagagtcaa taagcaggga taactttgtg
       61 aaaaaacagc ttcttctgag ggcaatttgt cacaacacct tctctagaac cagcatggat
      121 aaaggcctac aaggcgctct aaaaaagaag atctaaaaac tataaaaaaa taattataaa
      181 aatatccccg tggataagtg gataacccca agggaagttt tttcaggcat cgtgtgtaag
      241 cagaatatat aagtgctgtt ccctggtgct tcctcgctca ctcgagggct tcgccctgtc
      301 gctcgactgc ggcgagcact actggctgta aaaggacaga ccaaacatca tggttctgtg
      361 ttcattaggt tgttctgtcc attgctgaca taatccgctc cacttcaacg taacaccgca
      421 cgaagatttc tattgttcct gaaggcatat tcaaatcgtt ttcgttaccg cttgcaggca
      481 tcatgacaga acactacttc ctataaacgc tacacaggct cctgagatta ataatgcgga
      541 tctctacgat aatgggagat tttcccgact gtctcgttcg cttctcagtg gataacagcc
      601 agcttctctg tttaacagac aaaaacagca tatccactca gttccacatt tccatataaa
      661 ggccaaagca tttattctca ggataattgt ttcagcatcg caaccgcatc agactccggc
      721 atcgcaaact gcacccggtg ccgggcagcc acatccagcg caaaaacctt cgtgtagact
      781 tcctttgaac tgatggactt atgtcccatc aggctttgca gaactttcag cggtatacca
      841 gcatacagca tgtgcatcgc ataggaatgg cggaacgtat gtggtgtgac cggaacagag
      901 aacgtcacac cgtcagcagc agcggcggca accgcctccc caatccaggt cctgaccgtt
      961 ctgtccgtca cttcccagat ccgcgctttc tctgtccttc ctgtgcgacg gttacgccgc
     1021 tccatgggta ttttcagtgt tgccaccatc gtctgcagct ggctgacgta ccaggagtca
     1081 gagagcggaa ccagccggtg agtctgctga ccggcgggca ttctccccgc cgtcctggca
     1141 gctttttcgg tccgttgttt cagggtcgca agctgcacaa acggatacgg aggcgcaagc
     1201 gaaaaatccc cccgcgtcag cgccagtgct tcattaatgc gtgctccggt gttccacagt
     1261 gtggccagca gcatcttgcg gtgcagattc gggacgtaat ggagcagggc actcacttcc
     1321 ggagccagca gatattttgg cagttcatca tggaccatcg acatctggcg aagtgccaga
     1381 gctgccggat aatcaatggc aacaggcagc gatgcaggct gtccggcaga atacactgcc
     1441 ggtaccatga ctgcagactg gctgtgtata acggagcctg acatttatat tccccagaac
     1501 atcaggttaa tggcgttttt gatgtcattt tcgcggtggc tgagatcagc cacttcttcc
     1561 ccgataacgg agaccggcac actggccata tcggtggtca tcatgcgcca gctttcatcc
     1621 ccgatatgca ccaccgggta aagttcacgg gagactttat ctgacagcag acgtgcactg
     1681 gccaggggga tcaccatccg tcgcccgggc gtgtcaataa tatcactctg tacatccaca
     1741 aacagacgat aacggctctc tcttttatag gtgtaaacct taaactgcat ttcaccagtc
     1801 cctgttctcg tcagcaaaag agccgttcat ttcaataaac cgggcgacct cagccatccc
     1861 ttcctgattt tccgctttcc agcgttcggc acgcagacga cgggcttcat tctgcatggt
     1921 tgtgcttacc agaccggaga tattgacatc atatgccttg agcaactgat agctgtcgct
     1981 gtcaactgtc actgtaatac gctgcttcat agcacacctc tttttgacat acttcgggta
     2041 tacatatcag tatatattct tatgccgcaa aaatcagcgc gcaaatacgc atactattat
     2101 ctggctttta gtaagcctta tgtattttac ctttcgttat gttaaccaat aaaaattaaa
     2161 atctgcctta taaaaacaaa gcgtaattac cgcattcccg tttcgtatgg atccgacttt
     2221 atttgaataa acgactttcc ggaagtgtac cacctgagaa aatcatcata tcgctgtttt
     2281 ccgcccctgc gcggcgcatc acagtccaga gatacaacac aacatatagt gccatgggca
     2341 actgaatgag gcactgcatg ctgtgctgac gggatgtaac gctgtgcgga gggagaggaa
     2401 gatccgtaac caccagaaac cggatctgac caggaaaaga gaaagagtgt atgtaagttc
     2461 tgtactgtat gtatgaattg tattgtatgt attgtgtatt tttcataccg ggcatacgat
     2521 ggcgccattc cctgagaaac cggagccaga ccatgacatc cctctccccg gacaccgtcc
     2581 gccgcattga agacgccgcc gccgccctga tcgccgccgg caccccgaac ccgaccaacg
     2641 agcaggtccg tcagcacctc ggcggcggct ccctgtccca catctccccg gtgatgcgcg
     2701 cgttccgcgc ccgccagcgc gagcaggcca cgcccctgcc gccggagctg gcgcagctgc
     2761 tgaccggcca gctggggctg ctgtggcagg cggccgtgaa acaggctgaa gccggggccc
     2821 tggcggcgcg cgagcaggcc gacgacgaca ttgcccgggc cgacaaggag cgggatgagg
     2881 cgctggcgaa cgtggccgcg ctggaaagcg agctggcggt gctgcgcgag gtggtggccg
     2941 aacgtgaccg cctgctgcag gaggtacgcg agctacgcgc ggaggcgctg ccgctgcgcg
     3001 agcaggtggc ccgcctgacc gccaccggcg aacacctggc ggcgcagctg caggacacga
     3061 aggcggagct gaaggaagcc cgggaggacg gccgccagct tcagaccgaa ctgctggctc
     3121 tggcccgcca ggacgggaag gtgaagaaat gagacaggaa agagtattac gtataaaggc
     3181 atttcgacgg gcactggagc ttggtggcgg tgaacgggct gacgggtgtc gtcattttcc
     3241 ccgctggaaa acggaactga ggaatttccc gcacagcagc tgcgatttag ccagcaatac
     3301 tctggcgtac tacctcaaag aacgagagag atgccatccg tgcatcattt ttatggaagg
     3361 gaacgctggc tttcatgaag cggaaaactc aacagttcac gggcatgtca tcgtattgct
     3421 ggacggagag tacatcgatt tgacgctgga tcaattcgac gaatatccgg actacattcc
     3481 ttacgaacct gtcgaatctg acgggcagat tggtaagctg ttgcgtaata tcatgaagca
     3541 tgaggacccg gtaaaaaccc gccagattga tctcgatggt ggcgaagagc tgtatgcctg
     3601 gttacgcgat accgccgacg aagtgctggc agcagatccg gaatggcagg cccgggaacg
     3661 gtcgatagaa gaagcaaggg aagccgctat tacggtgttc ccgtttctgt ctgacgtgca
     3721 aaaaactgag tgcgaacagg gcccggacag tcaggaggct gccagatgaa gatttatcat
     3781 tacactgacc tgaacggcct gaagggcatc atcgaatcag gctcactgtg ggcgacgcac
     3841 ttttcctttc tcaatgacag taatgagctg actcacggca tgaactgcct tgagaatgcc
     3901 ctgcagtacc tgagggacga ctttaacccg aaaaccctta agttcatcga acaggcaccc
     3961 ttataagcgg ttgtgtatgc tgtttccctg gaatgattta tccacatatc cggaggctcg
     4021 atccagtaaa cagatccatg aatgatcaac aaaggatcca ttaaagatcc ccataccgct
     4081 gcaaaccttg tcactcatgg gccgggacca cgatcacata agcagtggca tgttactgat
     4141 aaactgtaac atgctaatga taagctgtat tcagtaatcc atatactgaa gtaagttaat
     4201 gacataaact atggtcagta cgccagactc agctgttaaa tacaggctgc aggtttttct
     4261 tcagtcagtt agcggggctc tgacacacga tttgctgttt attcttttac tgtccacagg
     4321 caggaggctt tctggaaaac gaaaattcag acatcaaaaa actgttcggc gaggtggata
     4381 agtcgtccgg tgagctggtg acactgacac caaacaataa caacaccgta caacctgtgg
     4441 cgctgatgcg tctgggcgtt tttgtaccga cccttaaatc actgaagaac agtaaaaaaa
     4501 atacactgtc acgtactgat gccacggaag agctgacacg tctttccctg gcccgtgctg
     4561 agggattcga taaggttgag atcaccggcc cccgcctgga tatggataac gatttcaaga
     4621 cctgggtggg gatcattcat tcctttgccc gccataacgt aattggtgac aaagttgaac
     4681 tgccttttgt cgagtttgca aaactgtgtg gtataccttc aagccagtca tcacgcaggc
     4741 tgcgtgagcg catcagccct tccctgaaac gcattgccgg taccgtgatc tccttttccc
     4801 gcaccgatga gaagcacacc cgggaataca tcacccatct ggtacagtca gcctactacg
     4861 atactgaacg ggatattgtt cagttacagg ctgatccccg cctttttgaa ctgtaccagt
     4921 ttgacagaaa ggtccttctc cagcttaagg cgattaatgc cctgaagcga cgggagtccg
     4981 cccaggcact ctacaccttt atagagagcc tgccccggga tccggcaccg gtatcgctgg
     5041 cgcggctgcg tgcacgcctc aatctgaagt ctcctgtatt ttcccagaac cagacggtca
     5101 gacgggcaat ggagcagctg cgcgagattg gatatcttga ttacacggag atccagcggg
     5161 ggcggacaaa actcttctgc attcactacc ggcgtccccg gttaaaagca ccgaatgatg
     5221 agagtaagga aaatccgttg ccaccttcac ctgcggaaaa agtcagtccg gagatggcgg
     5281 agaagcttgc cctgcttgag aaactgggca tcacgctgga tgacctggaa aaactcttca
     5341 aatcccgctg aacataaact gtagtcagtg aagagtgttc ctttactgac tacagcttat
     5401 attatcaggt gcagtgagtg gtctgctcac tgcagtttat attcagtttc ctgcagtgct
     5461 gcctgtagct gagctgtcat ctgccggtcc cttacgtgag tcaccccgta acctgatgct
     5521 gaggcattgc tcccttcata aaacatgact tactcactac agcttatata catgctccag
     5581 cttatgttat gtctgttctg ctgaccacag cttatataag gaagcgcgta tgccgtcacc
     5641 tcaggggagg cagtgtacgc aggatctccg cagcatcccg cccgtcacct gtgaaaggca
     5701 ctgccagcgt ggcagccata tccagcgcaa acactctggt ataaacctcc atcgaacgtg
     5761 gatccctgtg acctgccagg gcctggatga ctttccgggg ctggcggtga tagagcatat
     5821 gcatgatata gctgtgccgg aaggtgtgtg gtgtgaccgg aatcgaaaag tgtactccgt
     5881 cagcttcggc ccgtctgaca gcctgcttca gccagttgcg catggtttcg tcggtcacgg
     5941 cccataatgg ttcacgacga cggggccggg tggtgatcat ccagctttcc atctgcctga
     6001 catagcttat atccgtcagc ggaaccaggc ggacttcatc ttttggcggg cgtccgcgtc
     6061 gcgcacgcac tttttcggac aggatccgca caaacggcct tgctccatcc aggtcaaatg
     6121 attccggcgt cagcatccgg gcttcgccaa tacgcattcc ggtattccag agggtggcga
     6181 acagcatatg gtgacgctga tccggcatat agaaaagaag ggcactcact tccggggcca
     6241 gcaggtaggc cggtgtggcc cctgtggaag tggccattct tctcagggag agcgctgttg
     6301 caaaatccac ccccggcgca atgggtaaca ggaagacgcg ttctggtgaa ttctgcaggg
     6361 gaatgacatt gttcatgagt tatttttcac gtagcacacg cactgtttat ggatacaggt
     6421 attctgacct gaacgcccgg atccttccac gacaataaat cgcagcgtgc ggatcctttt
     6481 gtttgcttat cacgcattat tgcgggtaaa accaaatacg taaaggtacg ttttgcagca
     6541 gggatcccgt attaagccag tcgtggcgcg ggctgagggc tggtttgccg tttttgggag
     6601 aattggggta atgccaggac gctaatgatc tatcaatgga tggatgtaag tgacaattga
     6661 aaactgaaaa atgcgctatg tagattctga cagatttatg cacctgaaat acgttcaaaa
     6721 tccacatagc gtaactctga ttttccttac cagaaaaaag agggggaata accagtatct
     6781 atccccccta cacaattgca gaataataaa ttataaaaat aagaactcag gatttatata
     6841 catgattaca tgtaatatta ctgagaaata ttttgagata aaaccttttt gctgattatt
     6901 ttaaatgttt tgatttcatc atctgtaagg ttggtccata tttttttaaa tagcgtccat
     6961 atatccttgt ggtccgctgt aaatacaaat taccgtctga agtcagataa atattcttac
     7021 tacgtcgatc ttccttactt tccatacgtt taataaaacc aaatttttcc agagaatcac
     7081 aagctctggt gatatatgtt ctggaaacat ctaatctttc actgagttca gtcgaagata
     7141 agcagaaatt atcagtactg gataacaagg ctagaagcat aagcgttgta tcgtttatac
     7201 catccccctt cagggaggca ttaatatatc agtaagtttt ttggtaactc gcatacaaag
     7261 ttgtaaaaga agatgttcct gtgctggata taattgctca ttctgttgtt gatagataat
     7321 aa
//
LOCUS       DAZKIP010000183         7313 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZKIP010000183 DAZKIP010000000
VERSION     DAZKIP010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.185"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..99)
                     /gene="hokD"
                     /locus_tag="ACHOUK_004397"
     CDS             complement(<1..99)
                     /gene="hokD"
                     /locus_tag="ACHOUK_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFP9899713.1"
                     /translation="MKQQKAMLIALIVICITVIVTALVTRKDLCEVR"
     gene            complement(345..449)
                     /locus_tag="ACHOUK_004398"
     CDS             complement(345..449)
                     /locus_tag="ACHOUK_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899714.1"
                     /translation="MSDDISLAMEGALAVIAVVGVYCLVVFLMDRLGN"
     gene            complement(565..1149)
                     /locus_tag="ACHOUK_004399"
     CDS             complement(565..1149)
                     /locus_tag="ACHOUK_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFP9899715.1"
                     /translation="MTTFTKEQLISHVSENVKAMKFAVKQTAFKNSLEAIELDLALAL
                     VAQASLEAEPVLYMNRFTGKTFSLEEQPGADKEPEIYVPLYAAPPDSAAMLQAGNFRE
                     KKGSSTNNFREISETSTNYPVTLDDWISCSERMPDDGQHVIILCDGAFVLYAQYRDGE
                     FFDVVRNGDEFFETQSRNVTDWMPLPEPPQEVRQ"
     gene            complement(1206..1601)
                     /locus_tag="ACHOUK_004400"
     CDS             complement(1206..1601)
                     /locus_tag="ACHOUK_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFP9899716.1"
                     /translation="MAKPFTHEQREELKARIIGLVRKNERMTISQLERATGAGWHSVR
                     RCLVDVLACGDLYMPGKYGVFTSEQVYRVWRKAAEKATDQTLIRKLPDGEIRRYDRQQ
                     NIICGECRKSEVMLRVLAFYQGNFQEAVL"
     gene            complement(1617..2386)
                     /locus_tag="ACHOUK_004401"
                     /pseudo
     CDS             complement(1617..2386)
                     /locus_tag="ACHOUK_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
     gene            complement(2412..3152)
                     /locus_tag="ACHOUK_004402"
     CDS             complement(2412..3152)
                     /locus_tag="ACHOUK_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9899717.1"
                     /translation="MKNIAAVGVLERIRRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRLNHQVRVEKILNRAGIQPLHRKCSFGNYRVQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHDGYDNGQSGEKFLQE
                     LCGVDLLVLDEIGMQRDTRNEQVTLNQIVDRRTASMRSVGMLTNLNHAAMSTLLGDRV
                     MDRMTMNGGRWVNFNWESWRSNVGRQGM"
     gene            complement(3159..4121)
                     /locus_tag="ACHOUK_004403"
     CDS             complement(3159..4121)
                     /locus_tag="ACHOUK_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9899718.1"
                     /translation="MSSKLHGLVWEGCAFTGMILSRVAVMARLADYSNDEGVSWPAIE
                     TIRRQIGARSESTVKSAIAELAKEGWLTKEERKVGGRNVSNIYRLNVEKLEAAAAAAR
                     ESYKPKRKISPAKNDPLTVDPSNIAPSTVDPSNFDGSTVDNKLPIRGAMIDPDPSVLK
                     PDPSDKRSSCPDASQPDPQTAEQNFLTRHPDAVVFSAKKRQWGSQEDLVCAQWIWGRI
                     VSLYEQAASDDGEITRPKEPNWTAWANDVRTMRMLDGRTHRQICEMFGRLQRDSFWVK
                     NIMSPAKLREKWDELVIRLGRSPAQRCVNHISEPDTEIPPGFRG"
     gene            complement(4144..4569)
                     /locus_tag="ACHOUK_004404"
     CDS             complement(4144..4569)
                     /locus_tag="ACHOUK_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFP9899719.1"
                     /translation="MKIKHEHIRMAMNVWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDAVEKMQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVASAVVLYDQMNRGGPAGNAVVMH"
     gene            complement(4566..4868)
                     /locus_tag="ACHOUK_004405"
     CDS             complement(4566..4868)
                     /locus_tag="ACHOUK_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9899720.1"
                     /translation="MTLKEFIKSLRVGDAKKFAARLGVSPSYLSQMASGRTAISPTRA
                     LMIESATEGQVSRAELRPHDWELIWPEYASGIRLGQTHVVHAEGDCSACLSDGVDS"
     gene            4966..5337
                     /locus_tag="ACHOUK_004406"
     CDS             4966..5337
                     /locus_tag="ACHOUK_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899721.1"
                     /translation="MQKKEIRRLRLKEWFKDKTLPPKEKSYLSQLMSGRASFGEKAAR
                     RIEQTYGMPEGYLDAEYAEQPGGSPPHAGLTSNQLELLQIFSAFPEDEQRQIISELKQ
                     KKESMEDLIARWIAAQKCRRA"
     gene            5358..5549
                     /locus_tag="ACHOUK_004407"
     CDS             5358..5549
                     /locus_tag="ACHOUK_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stability determinant"
                     /protein_id="HFP9899722.1"
                     /translation="MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDE
                     VERRMAERFAKMRKERSKQ"
     gene            5551..5829
                     /locus_tag="ACHOUK_004408"
     CDS             5551..5829
                     /locus_tag="ACHOUK_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9899723.1"
                     /translation="MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLS
                     EHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVEVVNVIHARRQFP"
     gene            6182..6481
                     /locus_tag="ACHOUK_004409"
     CDS             6182..6481
                     /locus_tag="ACHOUK_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899724.1"
                     /translation="MALYIDISAIAGQVRVIRAVTKRYAPLLQKVSDECTEDIVNDFV
                     IELRGLIFSYKVTTIFADGSRETVRALRLKGCVKDFATTFWARKLDCIHNQFPLE"
     gene            6553..6771
                     /gene="ydfC"
                     /locus_tag="ACHOUK_004410"
     CDS             6553..6771
                     /gene="ydfC"
                     /locus_tag="ACHOUK_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFP9899725.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMHQKINAAQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
BASE COUNT         1817 a         1978 c         1654 g         1864 t
ORIGIN      
        1 tcgtacctcg cagaggtctt tcctcgttac cagtgccgtc actatgacgg taatacagat
       61 gacgatcagg gcgattaaca tcgccttttg ctgcttcata gcctgcttct ccttgccttt
      121 cggcgcgtaa gaggctaacc tacatgtgtc tagcatgaaa ttggcctcag attaatgtta
      181 ggcgtcttgc aggacgcgta atgttaactg gggcttttct ctgtctgcct tacggtggca
      241 tgcccgaggc agacagcctc aagcacccgc agcaattcta cttaactctc gctttaccgc
      301 aaaccgtttt tacccgatat gggaattccc atatcgtaat gaattcagtt ccctagtcga
      361 tccatcaaaa acacaaccag gcagtaaacg cccacaacag caataacagc cagcgcacct
      421 tccattgcca gtgaaatatc atccgacata ttccctcctt tggtgtgaat cccggcgaac
      481 gtttttaccc ccaccgacaa ataacatata ctaaaaaatc aatagctata gcaacgcctg
      541 taattgcaaa ggcttcaggc cagatcattg gcgcacctcc tgcggcggtt ctggtagcgg
      601 catccagtcg gttacattgc ggctctgtgt ttcgaaaaat tcatcaccat tacggactac
      661 atcaaaaaac tcaccgtctc gatattgcgc ataaagaacg aatgcgccat cacataaaat
      721 aattacgtgc tgaccatcat ccggcattcg ctcactacag cttatccaat catccagagt
      781 taccggatag ttggttgacg tttccgagat ttcccgaaaa ttattggttg acgaaccctt
      841 tttttcccga aagtttccag cctgaagcat ggcggcgctg tctggcgggg cagcatatag
      901 cggcacgtat atttccggtt ccttatcagc accgggttgc tcttccagtg agaatgtctt
      961 tccggtaaat cgattcatat aaagcacggg ctctgcttcc agcgaagcct gagcaacaag
     1021 ggccagtgct aaatccaact caattgcctc gagagaattt ttgaatgctg tctgttttac
     1081 tgcaaatttc atcgccttta cattttcact aacatgactg attaactgct cttttgtaaa
     1141 agtggtcatc tcattctcct ttgatgcgaa tgccagcgac aattgaagcc tgatagctaa
     1201 ttcactcaca gtaccgcctc ctgaaaattg ccctgataga acgccagtac acgcagcata
     1261 acttcactct tccggcactc gccacagatt atgttctgtt gtctgtcgta gcggcgtatt
     1321 tctccgtctg gtaactttcg aatcaatgtc tggtcggttg ctttctccgc tgccttacgc
     1381 catacgcgat acacctgttc tgatgtaaaa acaccgtatt taccgggcat gtataaatcg
     1441 ccacaagcca gtacatccac aaggcaacgt ctgactgaat gccagcctgc tcccgtcgct
     1501 ctctccagtt gtgatatcgt catgcgttca tttttgcgta ccagcccgat aattcgggcc
     1561 ttcagttctt cacgctgttc gtgtgtaaaa ggtttcgcca taagcgcctc cggctatcac
     1621 ttttccgata caacacggcg ggaagaatca gtaatctgtc gaacaatatc ccggtgcttg
     1681 ttcagctccc gcagcgcggc gcagactcgc tcccacttct gaacatcact tttcgccctg
     1741 cgcagcgcca ggtttgccct gcgcagggac ggaaaaatca gctcatctgc ttgcgtttcg
     1801 gtaaacgatg gcaacggctg cacaatgtcc gccacagttt ctgttttaat ttcttcctgt
     1861 gttgcggctt cccggactgg taacgcagca cctgctggct gaggaaaggc cttaccatca
     1921 tttttcgtta ccggcgcggc tttcggatct gctggtaaat taccccccga catgcagtaa
     1981 cgaaatttac cgttctgatt aacgcgtgcc agccgccccg ttgcggttac caccgccagc
     2041 gtggaagcaa ccttgcgagt actgacgccg aacttacccg ccagttcctc acacgtttta
     2101 gcaccatcct gaccgataaa ctcaaccatc atgtctgcgg taacttttgg agcgacctct
     2161 tcggttacca catccggcgc ttcaggttgt agtgcctgcc cttcggttac cccggcttca
     2221 ccttcgacag ccagaaacca ggtgtgaccc gttttatcaa caacgccatt tttttgagtt
     2281 cccacagttc gttaagaact tcttcacggc tgatatcaag ccgcgccgcc agttcaacag
     2341 aattggcttt tcccatcgct ttcagtgcat gcaatacagt ttccatcgaa aatttacctc
     2401 gtcaaaaatt ctcacatacc ctgacgtcca acgtttgacc gccagctctc ccagttaaaa
     2461 ttcacccaac gaccaccatt catggtcata cggtccatca cccgatctcc gaggagtgtg
     2521 ctcatcgctg cgtgattcag gttcgtcagc attccgacac tacgcatcga agccgttctg
     2581 cggtcgacta tctggttcag cgtgacctgc tcgttgcgcg tatcccgctg catgccaatt
     2641 tcatcaagga ccagaaggtc aactccacaa agctcctgta aaaatttttc cccggactgg
     2701 ccgttgtcgt agccgtcatg caacacgctc atgacatcgg acacggtgac gataatcacg
     2761 cttctcccct tcgccatcag ccgattgcca atcgctgctg ccaggtgatt tttaccggta
     2821 ccaggtttac cgctgaacac gaagtttgta catccggtca tcaattcatc ggcaatggat
     2881 ttcgcctggc tcagagcatg gcgctgaccg tcgttctgca cccggtagtt cccgaatgag
     2941 cacttcctgt gaagcggctg gatgcccgca cggttcagga ttttttcaac ccgcacctga
     3001 tgattcaggc ggttaatctc ctcgctgcgt tttcgtcctt cagcaagttg ccattcccgc
     3061 cactcctcca ccgtccggta cggtggaacc gccccctgtg gtgcaagtct gcgaatacgt
     3121 tcaagaaccc caactgccgc aatgtttttc atgacacgtc accccctgaa tcccggcggt
     3181 atttcagtgt ccggttcaga aatgtgattc acgcaacgct gcgcgggcga acgccccagg
     3241 cggataacca gttcatccca tttttcccgg agttttgccg gactcatgat gttttttacc
     3301 cagaacgaat cccgctggag acgcccaaac atttcacaaa tttgtctgtg agttctgcca
     3361 tccagcatcc gcattgtccg cacgtcattg gcccatgcag tccagttggg ttctttcggt
     3421 ctcgtgatct cgccatcatc gctggccgcc tgctcgtaaa gactcacgat tcgtccccag
     3481 atccactgtg cgcacaccaa atcttcctga cttccccact ggcgtttttt cgcactgaac
     3541 acaaccgcgt cagggtgtcg ggttaaaaaa ttctgttcag ccgtctgcgg gtccggttgc
     3601 gaagcgtccg gacaagaaga tcttttatct gacggatcag gttttaatac tgacggatcg
     3661 gggtcaatca tcgcccccct aatcggcagt ttgttatcaa cagttgatcc atcaaaattt
     3721 gacgggtcaa ccgttgaggg ggcaatattt gacgggtcaa ctgttaacgg gtcatttttt
     3781 gccgggctaa tttttctttt cggtttatat gactcacgcg ccgccgcagc agctgcttcg
     3841 agtttttcca cattaagccg atagatattg cttacattac gcccaccgac cttacgctct
     3901 tccttcgtca gccagccctc tttcgccagt tctgcaatag ccgatttcac tgtggattca
     3961 cttcttgcac cgatctgacg ccggatagtt tcaatggcag gccatgacac gccctcgtca
     4021 ttgctgtagt ctgcaagacg ggccataacc gccaccctgg ataagatcat gccggtgaag
     4081 gcgcaccctt cccagacaag accatgaagc ttgctgctca taaaaccccc gaacaccgtg
     4141 cttttagtgc atcaccacag cattccctgc cgggccgccg cgattcatct ggtcatacaa
     4201 aacaaccgct gacgcaacaa aatcatcgac atccttcacc agccgatccc tccgttcgac
     4261 gatctcacgg taatattcag aactgtggct gcgcatacgg gccaccagca aaggcggcat
     4321 cgccttttcg atcgccggta acagagcctg cattttttca acagcatcag gggtgtcttt
     4381 atccagccaa cggaaaattt tctgggtatt acgggccagg gcttccggat ggctgtcgtc
     4441 atacagttcc gggaacgtca ttcccagctc gaaatacgct ttggtaattt tcgcagccgg
     4501 tactttttcg ccgtccggat gcgcccagac attcatcgcc atgcggatgt gttcatgctt
     4561 gattttcatg aatcaactcc atcagataag catgcactac aatcaccttc agcatgaact
     4621 acatgtgttt gccccaaacg aatgccgctc gcatactcag gccaaataag ctcccaatca
     4681 tggggtcgta gctccgccct acttacttgg ccttccgtcg cagattcgat cataagggcg
     4741 cgggttggag atatagctgt tcgtccagac gccatttgcg ataagtaaga tggcgataca
     4801 ccaagtctgg ccgcgaattt cttagcatca ccaaccctta atgatttaat aaactctttt
     4861 aatgtcatac cttcctcggt ttagtgtttt tttgcgagtt tagtgtttaa taaaccatta
     4921 agtcaagtat ttgcttgttt agtgattact aaagataatt accacatgca aaaaaaagaa
     4981 attcgccgtt tacgtctcaa ggagtggttt aaagataaaa ctctgccacc caaagagaag
     5041 agctacctat ctcaactaat gagtgggaga gcctcgtttg gagaaaaggc tgccagaaga
     5101 atagagcaaa catacgggat gccggaaggg tatctggatg cggaatacgc agaacaaccg
     5161 gggggttctc caccacatgc agggttaacg tctaatcaac tggaattatt gcagattttt
     5221 tcagccttcc ctgaggatga gcaacgccag ataatcagcg agttaaagca gaaaaaagaa
     5281 tcaatggaag atctcatagc gagatggatt gcggcgcaaa aatgccgccg cgcctgagtt
     5341 ataaaaccgg aggaaacatg aatagagccc tttcaccaat ggtttctgaa tttgaaacca
     5401 ttgaacaaga aaacagttac aacgaatggc tgcgtgcgaa agtagcaacg agccttgcag
     5461 atccgcgccc agcaattccc catgacgaag ttgagcgcag aatggcagaa cgctttgcta
     5521 agatgcgcaa ggaacggagc aagcagtaaa atgttacccg tgttatggct tgaaagcgca
     5581 gataccgacc tagatgatat aactagttat attgctcgtt tcgacataga tgcggctgaa
     5641 cgcttatggc agcgattaag gggttgtgtg ctgccgttat ccgaacatcc gtatttatac
     5701 ccaccaagcg acagagtacc tggcttgcgt gagattgtag cccaccctaa ctatataatt
     5761 ctataccgcg taacaacatc aagcgttgaa gtagtaaacg tgatccacgc aagacgccag
     5821 tttccctaac tttcactacc aatagaaaca taacaaccgc aacgacttta tcaaaagcgt
     5881 tgtgtttgtt atgtcccgcg gtttagtttt tacttgactt aagtttaatg tttattaaac
     5941 taaaaatacc aacccacccc gccccacaga acgcagggaa atacttcgag ttacccggca
     6001 gtggtcaggg gttaagtagc cagcccgagg cgtatgaaca tgacggcagg gttcaacttt
     6061 aataactatg cagcaggttt ttgttccgct accccggcgt taaggggaaa cagagggttt
     6121 ctcagtgggc gaagtcaaac atcagaatgg aaggcatccc gggatcggca aagaagcagc
     6181 aatggcgctt tatattgaca tcagcgccat tgcaggacag gtaagagtta tcagagcggt
     6241 aactaagcgg tatgcgcctt tacttcagaa agtctctgat gagtgcaccg aagatattgt
     6301 caacgatttc gtcatcgaac tgcgaggact catcttcagt tacaaggtga ccacaatttt
     6361 tgcagatggc tcccgcgaaa ctgtcagagc cctgcggctt aaaggatgtg tcaaagactt
     6421 cgccaccaca ttctgggcaa gaaaacttga ttgtattcat aaccaatttc ctctcgagta
     6481 acagacccct cagaggatac cacctcgcct gacgtggtta aaagcaggca acgctaacca
     6541 caaggagccg acatgcagaa acgagaaccc gtcatcatcg cgccagacta taccgatgat
     6601 gaactttatg agtggatgca ccagaaaatt aatgcagcgc aggatctgaa atgggccaat
     6661 gaagccaggg ctaagcaggc tgaaaatctg tccgctctgg agcaggatat caccaatctg
     6721 gaaaaagcag cggcattaag cattgccaga atgattacat acccgcgtta atagctaacc
     6781 aacgaagcta aggttggtaa ttaaggagtt ctccacgggt gaggtggagt gcgtgcgccg
     6841 gacacgggtg agcatacggc actgacagtt tactgaaagg atatttccct gaaaagtcag
     6901 accataacgc gaaagcgcac ggcgaggtag ctggttcata gatagcctgt cgttaaattt
     6961 tcgtcgaccg tgcgcttccg gttgtggcaa tccgcgaaat ggcgcggcgg taagtatgga
     7021 ggggttattc cttcccccgt tgaggacacc gggttgtcag gttgaccata cgcttaagtg
     7081 acaaccccgc tgcaacgccc tctgttatca attttctggt gacgtttggc ggtatcagtt
     7141 ttactccgtg actgctctgc cgcccttttt aaagtgaatt ttgtgatgcg gtgaatgcgg
     7201 ctgagcgcac gcggaacagt taaaaccaaa aacagtgtta tggggggatt ctctgtatcc
     7261 ggcgttaatt gttaactggt taacgtcacc tggaggcacc aggcaccgca tca
//
LOCUS       DAZKIP010000184         7217 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZKIP010000184 DAZKIP010000000
VERSION     DAZKIP010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.186"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(326..1462)
                     /locus_tag="ACHOUK_004411"
     CDS             complement(326..1462)
                     /locus_tag="ACHOUK_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFP9899726.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1465..1827)
                     /locus_tag="ACHOUK_004412"
     CDS             complement(1465..1827)
                     /locus_tag="ACHOUK_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFP9899727.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIASLSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2364..4277
                     /gene="mtlA"
                     /locus_tag="ACHOUK_004413"
     CDS             2364..4277
                     /gene="mtlA"
                     /locus_tag="ACHOUK_004413"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFP9899728.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVETLSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4372..5520
                     /gene="mtlD"
                     /locus_tag="ACHOUK_004414"
     CDS             4372..5520
                     /gene="mtlD"
                     /locus_tag="ACHOUK_004414"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFP9899729.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVDAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISDLDANSEVVSEAVTAYKAMQ"
     gene            5520..6107
                     /gene="mtlR"
                     /locus_tag="ACHOUK_004415"
     CDS             5520..6107
                     /gene="mtlR"
                     /locus_tag="ACHOUK_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFP9899730.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(6119..6328)
                     /gene="yibT"
                     /locus_tag="ACHOUK_004416"
     CDS             complement(6119..6328)
                     /gene="yibT"
                     /locus_tag="ACHOUK_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFP9899731.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            6613..6975
                     /locus_tag="ACHOUK_004417"
     CDS             6613..6975
                     /locus_tag="ACHOUK_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFP9899732.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1852 a         1799 c         1813 g         1753 t
ORIGIN      
        1 tgatctatag attaaatatt ttttaaatgt gactgtcatc cttcagataa taaagccatc
       61 atcgcaatag atggaaaagc actcctgctc ttttttgatt agagccgtcg caggaggaac
      121 aactcatatc atcattgcga tctcgacgat gtatagtctc atcctgcggc agaacaagac
      181 ggataaaaaa tccaatgaga gcacactcat ccagaactta ttaacataat gaatattaaa
      241 gaaaaatcat cgcagctaat gaagtaatat gcaaaaataa ttagtcctta caaggagcaa
      301 agttccataa tctcccccct ccccctcaat gatccaaata aagataatgc atccagctgg
      361 tcattcttag cagcactttc cgcatcaccg aaacatggct gaaatggtcg gagtaaaccg
      421 ccacctgggc gtagatgccg tcgggtaagg catcgatatc atcgttaggg tccagttcaa
      481 tggttcccag cacaccgtcc gtgccgggca cgaccgttaa tgattgcaat accccctgcg
      541 cctgataaga accgcctggc acgacaggta aaatactagt cagtttgccg tgaaacacct
      601 gcccaggtag cgcgttaaac accacttccg catcatcgcc gggtttcaga cgtaacagcg
      661 agttttgccg aaattgggcg acaatttgcc gtttttgctc ggggatgaag accatcaccg
      721 gacgcagcgg caaggcagct gcgtatgtac ctgggcggat cagtacctga gtaacgtagc
      781 cattgctcgg cgcgcgaatg acagtctgct caaggttata ttttgcttca gtaagttgcg
      841 ctcttaagct cacaatctgc gattgctcgc cgttaaccat actgtcgagc tggctctgga
      901 tctgcgcctg ctccgccacc gagcctttca ccagcgcatc ctgcgcgagg aaattttgcc
      961 gcgcatcgtc gatgtcacgt tccgagaacg gattcaccgc cgcctggctg cctttcaagt
     1021 aacgttgata atttttaaac agacggtcgc gctccgctga aacctgggtg gtgttggcct
     1081 gcgcttcagt gagctgcgca cgcagcgtct ttatattatg cgtcgccgtc atcaggtcag
     1141 cctgaagtct gtcaactcgc gcctggtaac gaaccgggtc gagcttaaaa agcacctcgc
     1201 ccttttgaat aagctgatta ttcttgtcag tgacttcagt aacaattccc gtcacctgtg
     1261 gcgtgatagg gatcgctatc actgcctttt gcgcggtaaa agtgtaaggg tggttgtagt
     1321 tcatcaataa aatcaaacca ctcaccagaa acacgcctcc cagcgccgcc gtcgccagcg
     1381 tccactgatt taccggaatg cggaagattt taaagaccgc ccacgccagc gccacgtaag
     1441 ttaaaacaat caatagatcc ataattagat ctccgggaac gtggaatagt cagtcttttt
     1501 ctcggcggcg agttgatgct ccagccggga aattcggtca gaaagtgagg cgatttccgg
     1561 gtcagtcgct ttctcctgcg acgcaacatg tgactgcata ccccagccac gctccggttg
     1621 atagagcgtc gcccagatcc acagaaacgg ccagataaca tgcagagtaa acaggctcac
     1681 ccagcccgcc gtatgaatag cgtcggcatg ggggtgattg cgctttttgg caatcagata
     1741 agggatgtca tgtatcgcga tgattccata aaaaatcacc agaaagacga agatcagcac
     1801 tcccaacgcg aaatagttta gaaacatatc tgcctcggat tcacgtttat cagtgttgtt
     1861 tttgggctgg cagccagaag ggagtcaggc tgatattttg acaataatcc gggttcgcga
     1921 ttctcgccat aacaccaaag aataattttt agaggtgatg agttgcttag ttacataacg
     1981 attgtatgac gaaggcataa catgctgtag atcacatcag gtgaacgccg taagaaaata
     2041 tcttgtgatt cagatcacaa agattcaaca aaccatcaaa acaaaaatgt gacactactc
     2101 acatttaaat gccattttta gcgaaaatcg ccgccttgtt gcttttttac acaagcgttt
     2161 tgtgatgaac gtcacgtcaa ttacctctct accccctata tttatgtgat tgatatcaca
     2221 caaaaggccg tcgactggac agttaaccga ttcagtgcca gatttcgcag tatctacaag
     2281 gtccggctac ctctgccgcc acattaacaa aaaacctcgg gcttccagcc tgcgcgacag
     2341 caaacataag aaggggtgtt tttatgtcat ccgatattaa gatcaaagtg caaagctttg
     2401 gtcgtttcct cagcaacatg gtgatgccaa atatcggcgc gtttatcgcg tggggtatca
     2461 tcaccgcgtt atttattcca acagggtggt taccgaacga gacgctggcg aagctggtcg
     2521 ggccgatgat cacttatctc ctgccgctgc tgatcggtta taccggtggt aagctggtag
     2581 gcggcgaacg tggcggcgta gtcggtgcca tcaccaccat gggcgttatc gtcggcgcag
     2641 acatgccgat gttcctcggt tctatgattg caggtccgct gggcggctgg tgcattaagc
     2701 acttcgaccg ctgggtagac ggtaagatca aatccggttt tgagatgctg gtgaataact
     2761 tctccgcagg catcatcggg atgatcctcg ctattctggc attcctcggc attggcccga
     2821 ttgttgaaac cctgtccaaa atgctggctg cgggcgttaa cttcatggtt gtccatgaca
     2881 tgctgccgct ggcgtctatc tttgttgaac cggcgaaaat cctgttcctc aacaacgcca
     2941 ttaaccacgg tatcttctcg ccgctgggta ttcagcagtc ccatgaactg ggtaaatcaa
     3001 tcttcttcct gattgaagct aacccaggtc caggtatggg cgtgctgctg gcgtacatgt
     3061 tctttggtcg tggtagcgct aaacagtctg cgggcggtgc ggcaatcatc cacttcctgg
     3121 gtggtatcca cgaaatctac ttcccgtatg tgctgatgaa tccgcgtctg atcctcgcag
     3181 tcatcctcgg cggtatgact ggcgtgttca cgctgactat cctgggcggt ggtctggttt
     3241 ctccggcatc tccgggttct atccttgctg tactggcgat gacaccaaaa ggtgcttatt
     3301 tcgctaacat cgcgggtgtg tgtgcggcga tggctgtctc cttcgttgtc tctgctattt
     3361 tgctgaaaac cagcaaagtg aaagaagaag atgatattga agcagcaact cgtcgtatgc
     3421 aggacatgaa agctgagtct aaaggtgcat ctccgctgtc tgcgggcgat gtgactaacg
     3481 acctgagcca cgtacgtaaa atcatcgttg cctgtgacgc cggtatgggt tccagtgcga
     3541 tgggcgcagg cgtgctgcgt aagaaaattc aggatgcagg tctgtcacag atttcggtca
     3601 ctaacagcgc gatcaacaac ctgccgccag atgtggacct cgtcatcact caccgtgacc
     3661 tgaccgaacg cgctatgcgc caggttcctc aggcacagca tatttcgcta accaacttcc
     3721 tcgacagcgg cctgtacacc agcctgactg aacgtctggt tgctgcccag cgacatactg
     3781 aaaacgaagt gaaagtgaaa gacagtctga aagacagctt tgacgattcc agtgctaacc
     3841 tgttcaaact gggcgcggag aacatcttcc tcggtcgcaa agcagcaacc aaagaagaag
     3901 cgattcgttt tgctggcgag cagctggtga aaggcggtta cgttgagccg gagtacgttc
     3961 aggcgatgct ggatcgtgaa aaactgaccc cgacttatct gggtgagtct atcgcggtgc
     4021 cacacggtac ggttgaagcg aaagatcgcg tactgaaaac gggcgtggtg ttctgccagt
     4081 acccggaagg cgtgcgcttc ggtgaagaag aagatgacat tgctcgtctg gtgattggta
     4141 ttgctgcccg taacaacgag cacattcagg ttatcaccag cctgaccaac gcactggatg
     4201 atgagtccgt catcgagcgt ctggcacaca ccaccagcgt ggatgaagtg ctggaactgc
     4261 tggcaggtcg taagtaatcc aatcctaccc tctcctcacg gagagggttt gggtgaggga
     4321 aaagcctcac cccagccctc tcgggtaaaa acattgatga aggttaatac tatgaaagca
     4381 ttacattttg gcgcaggtaa tatcggtcgt ggctttatcg gtaaactgct ggcagacgcg
     4441 ggtatccaac tgacgtttgc cgatgtcaat caggtggtac ttgatgccct gaatgcccgt
     4501 catagctatc aggttcatgt ggtcggtgaa accgagcagg tagataccgt ttccggcgtc
     4561 gatgctgtca gcagcattgg tgatgatgtc gttgatctga ttgctcaggt cgatttagtc
     4621 actaccgccg ttggcccggt tgtgctggaa cgtattgctc cggctatcgc caaagggctg
     4681 gtgaaacgta aagaacaagg taatgaatcc ccgctgaaca tcatcgcctg tgaaaacatg
     4741 gtacgcggta ccacgcagct gaaaggtcat gtgatgaacg ccctaccaga agacgccaaa
     4801 gcgtgggtag aagaacacgt tggctttgtc gattccgccg ttgaccgcat cgtaccgcct
     4861 tcggcttcgg caactaacga tccgctggaa gtgacggtag aaactttcag cgaatggatt
     4921 gtcgataaaa cgcagttcaa aggcgcactg ccgaacatcc caggcatgga gttaaccgac
     4981 aacctgatgg catttgtcga acgtaaactc ttcaccctga acacgggtca tgctataacc
     5041 gcgtacctcg gaaaactggc cggtcatcag accattcgtg acgctattct cgacgagaaa
     5101 atccgcgcgg tggtaaaagg tgcgatggaa gaaagtggtg cggtactgat caagcgctac
     5161 ggctttgacg cagacaaaca tgcggcgtac atccagaaaa tcctcggtcg ttttgagaac
     5221 ccgtatctga aagatgatgt agagcgcgta ggccgtcagc cgctgcgtaa actgagtgct
     5281 ggcgaccgtc tgatcaagcc attgctcggt acgctggaat acagccttcc gcacaagaat
     5341 ctgattcagg ggattgctgg tgcaatgcac ttccgcagtg aagacgatcc gcaggctcag
     5401 gaactggcag cactgatcgc tgacaaaggt ccgcaggcgg cgctggcaca gatttccgat
     5461 cttgatgcca acagcgaggt tgtatccgag gcggtaaccg cttataaagc aatgcaataa
     5521 tggtggacca ggcgcaggac accctgcgcc cgaataacag attgtcagat atgcaggcaa
     5581 caatggaaca aacccaggcc tttgaaaacc gtgtgcttga gcgtctgaat gctggcaaaa
     5641 ccgtgcgaag ctttctgatc accgccgtcg agcttttgac cgaggcggta aatcttctgg
     5701 tgcttcaggt attccgcaaa gacgattacg cggtgaagta tgctgtagaa ccgttactcg
     5761 acggcgacgg tccgctgggc gatctttctg tgcgtttaaa actcatttac gggttgggcg
     5821 tcattaaccg ccaggaatac gaagatgcgg agctgctgat ggcattgcgt gaagaactaa
     5881 atcacgacgg caacgagtac gcctttaccg acgacgagat ccttggaccc tttggggaac
     5941 tgcactgcgt ggcggcgtta ccaccgccgc cacagtttga accagcagac tccagtttgt
     6001 atgcaatgca aattcagcgc tatcaacagg ctgtgcgatc aacaatggtc ctttcactga
     6061 ctgagctgat ttccagaatc agcttaaaaa aagcctttca aaagtaagca acgtctgctt
     6121 actgcccctc tacctgcttc ggccgataaa gccgacgata atactccaga cgttgtagat
     6181 ataacggcac gctttcatcg ggtattccgg acggaatcgc gttacgggga ggaagttttt
     6241 tcagatactc ccggaacgcc tggcttgatg ccatgaaatc tacggcttta tcgataagaa
     6301 gcggaacgtt ttcacctaat ttgcccatga tgatatctcc gtattacccc gcgccgggaa
     6361 tgcgcggccg ccaattttag tttaggttcc cggtaaagat gacattaagg aaaacgtgct
     6421 gaatcctcaa taatcatccg ctgctgttta ttatcttttt tctatagatt tcttaattaa
     6481 tcaataaatt gcgtagtttt taagcatgtt aatgattacg ttacagaact attgcaatgt
     6541 catcagttgt gaacaaagca cctggtcgcg catactaggg gctataaatt tatctttatc
     6601 agaagccatc acatgaaaga agtcgaaaaa aacgaaatca aacgtctcag cgatcgtctg
     6661 gacgccatcc gccaccagca ggccgatctg tcgctggttg aagccgcaga caaatatgcc
     6721 gagctggaaa aagagaaagc cacgctggaa gcagaaattg ctcgcctgcg tgaagttcat
     6781 agccaaaaac tgagtaaaga agcacagaag ctgatgaaga tgccgtttca gcgcgcaatt
     6841 accaaaaaag agcaggctga tatgggcaag ctgaagaaaa gtgttcgcgg actggtggtt
     6901 gtgcacccaa tgaccgcgct gggccgtgaa atgggcctgc aggagatgac tggattctct
     6961 aagactgcgt tttaagcgcc tcagacgact ggttcgatca tatcaacccg catctggctt
     7021 gccttagctg gtcaatgcgg ggactctcct gtctactcgc ctaattacca aacaattcac
     7081 tccctttacg tcgatttacc gctggttgat attctgaaaa tgcattttta ccgaaatagc
     7141 cacctataaa caataaaaat atatagaaga tatcatttat taattaacaa gcagttacgt
     7201 ctcttttttt cggattt
//
LOCUS       DAZKIP010000185         7195 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZKIP010000185 DAZKIP010000000
VERSION     DAZKIP010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7195
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.187"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            319..1233
                     /gene="shuT"
                     /locus_tag="ACHOUK_004418"
     CDS             319..1233
                     /gene="shuT"
                     /locus_tag="ACHOUK_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     ShuT"
                     /protein_id="HFP9899733.1"
                     /translation="MPRIITRPFLFSPLTLCISAVASAAKTAVKRKKLFTAVLALSWA
                     FSVTAAERIVVAGGSLTELIYAMGAGERVVGVDETTSYPPETAKLPHIGYWKQLSSEG
                     ILSLRPDSVITWQDAGPQIVLDQLRAQKVNVVTLPRVPATLQQMYANIRQLAKTLQVP
                     EQGEALVTQISQRLERVQQNVATKKAPVKAMFILSAGGSAPQVAGKGSVADAILSLAG
                     AENVATHQQYKSYSAESLIAANPEVIVVTSQMVDGDINRLRSIAGITHTAAWKNQRII
                     TVDQNLILGMGPRIADVVESLHQQLWPQ"
     gene            1253..2590
                     /gene="hutW"
                     /locus_tag="ACHOUK_004419"
     CDS             1253..2590
                     /gene="hutW"
                     /locus_tag="ACHOUK_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HFP9899734.1"
                     /translation="MNANNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLAQT
                     WQEMINQTASPRKRLVYLHIPFCATHCTFCGFYQNRFNEDACAHYTDALIREIEMEAD
                     SVLHQSAPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
                     RIDACLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLF
                     GLPGQDAQTWGEDLAIARDIGLDGVDLYALNVLSNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDDAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMLIMRNAERNAQWRHTLQSGVETARVPLDELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIMREKP"
     gene            2603..3097
                     /gene="hutX"
                     /locus_tag="ACHOUK_004420"
     CDS             2603..3097
                     /gene="hutX"
                     /locus_tag="ACHOUK_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HFP9899735.1"
                     /translation="MSHVSLQEFLKTEPDGTLEVVAEQYNTTLLEVVRNLPSSTVVPG
                     DKFDTVWDTVCEWGNVTTLVHTADVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKEGSAMLKIFLGRDDHRQLLSEQVSAFHTLAASL
                     KEHA"
     gene            3097..3720
                     /gene="chuY"
                     /locus_tag="ACHOUK_004421"
     CDS             3097..3720
                     /gene="chuY"
                     /locus_tag="ACHOUK_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HFP9899736.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVIRHADAATKLAQ
                     QGVQVFTGDACDASVVAAACRAAGPDALIISTMGGAQDYLAHRTVIDEAEKAGISRMI
                     LVTSLGCGDSWPFLSERAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRIQNQECHGFVRRADVAAHIHELANAPALNQQVYSLIEPDLKPA"
     gene            3769..4761
                     /gene="shuU"
                     /locus_tag="ACHOUK_004422"
                     /gene_synonym="chuU"
     CDS             3769..4761
                     /gene="shuU"
                     /locus_tag="ACHOUK_004422"
                     /gene_synonym="chuU"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease ShuU/ChuU"
                     /protein_id="HFP9899737.1"
                     /translation="MLKDSFSSARVFMGLSLLLLALVLFGASQGALKISFDALFDEEY
                     RDIWLNIRLPRVLLAVLVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIV
                     LPFSFPVVLVLYEQMVFAIAGSLVVCAIIFLITQRHRDGSMMQLLLAGIAINALCGAA
                     IGILSYIGDEQQLRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQL
                     GDEEAHYLGVNVKRKRQQLLLVSSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRW
                     LIPCSALAGACLLLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            4758..5528
                     /locus_tag="ACHOUK_004423"
     CDS             4758..5528
                     /locus_tag="ACHOUK_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HFP9899738.1"
                     /translation="MISAQNLVYSLQGRRLTDNVSLTFPGGEIVAILGPNGAGKSTLL
                     RQLTGYLQPDSGECRLFNKPLNEWSITELAKHRAVMRQNSHMAFPFSVQEVIQMGRHP
                     HRTGNQDNETAQIMALCDCQALANRDYRQLSGGEQQRVQLARLLVQLWEPTPSPKWLF
                     LDEPTSALDIHHQQHLFRLLRQLVHERQFNVCCILHDLNLAAHYADRVVLMQKGKVIA
                     NGKPQDVLTQQALTMLYGADITVLKDPANHSPLIVLDH"
     gene            complement(5580..6227)
                     /gene="yhiD"
                     /locus_tag="ACHOUK_004424"
     CDS             complement(5580..6227)
                     /gene="yhiD"
                     /locus_tag="ACHOUK_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFP9899739.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(6291..6617)
                     /gene="hdeB"
                     /locus_tag="ACHOUK_004425"
     CDS             complement(6291..6617)
                     /gene="hdeB"
                     /locus_tag="ACHOUK_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFP9899740.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            complement(6733..7065)
                     /gene="hdeA"
                     /locus_tag="ACHOUK_004426"
     CDS             complement(6733..7065)
                     /gene="hdeA"
                     /locus_tag="ACHOUK_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFP9899741.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
BASE COUNT         1822 a         1803 c         1723 g         1847 t
ORIGIN      
        1 ggttatgaaa cctatcctgc gccctgtaaa ccaccttgtt tttatggtta tccccccatg
       61 cctcatcact aaattttgtc gttaacagtg ataatttttt cttctctttg ctctgcaagc
      121 ctcgatatct ctgggttctc agctttttcc cgcgatgaaa tttagtcgga aacaagaacg
      181 ttagctgacg aagcatcttg ataaaaatca aaaataaata cgcttgtaac gccttgcgta
      241 ttgaatcgac ggttgtattt catatataac tcaaatgata atcatgatca ttctcaattt
      301 gataagagtt ctcatattat gccaaggatc atcaccaggc cgtttctttt ttccccactg
      361 acgttatgta tttcagccgt ggcctctgcc gcgaagaccg cagtaaaacg taaaaaattg
      421 tttacggcag tactggctct tagctgggct tttagcgtaa cggctgccga acgtatcgtg
      481 gtcgcaggag gatcgctgac ggagctaatc tacgcgatgg gcgctggcga acgcgtggtt
      541 ggtgtcgatg aaacgacatc ttatccacca gaaaccgcca aactgccgca tattggctac
      601 tggaaacaac tcagcagcga aggcattttg tcacttcgcc cggatagcgt aattacctgg
      661 caggatgccg ggccacaaat tgtgctcgac caactacgag cgcagaaagt caacgtcgtc
      721 accctgccgc gtgtgcctgc cacccttcag cagatgtacg ccaacattcg ccaactggca
      781 aaaacgctac aggttcctga acaaggcgag gcgctggtga cacaaatcag ccaacgcctg
      841 gagcgagtac agcaaaacgt ggcgaccaaa aaagccccgg ttaaagccat gtttattctc
      901 tccgcaggcg gaagtgcgcc acaagttgcc ggtaaaggca gcgtcgcgga cgccattctt
      961 tcgcttgccg gagcagaaaa cgtcgctacc caccagcagt acaaaagcta cagtgcagaa
     1021 tcactgattg cggctaatcc agaagtgatt gtcgtaacct cacaaatggt cgatggcgat
     1081 attaatcggc tacgttctat tgctggaatt acccacactg ccgcatggaa aaaccagcgc
     1141 attatcaccg ttgatcaaaa cctgattctg ggaatgggtc cgcgtattgc tgatgtcgtt
     1201 gagtctttac accagcagct ttggccgcaa taaatggatt taaatcagac gaatgaacgc
     1261 aaataacacc ctggatctca cgccacattt tgctttagac ggtgatcagc catttaaaga
     1321 caggcgcgcc atgatgccgt ttcgtggagc cattccggtt gccaaagagc aactggcaca
     1381 gacctggcaa gagatgatca atcaaacggc atcacctcgt aagcgactgg tttacctaca
     1441 cattcctttt tgtgcgacac actgcacgtt ttgtggtttt tatcagaacc gttttaatga
     1501 agatgcgtgt gctcattata ccgacgccct gattcgtgaa atagaaatgg aagcggacag
     1561 cgtattgcat cagtctgccc ccatccacgc ggtctatttc ggtggcggta cgccttccgc
     1621 actttcggca cacgatttgg ccagaatcat caccacatta cgagaaaagc tgccactggc
     1681 ccctgattgt gaaatcacta tcgaaggacg ggtattgaat tttgacgctg agcgaatcga
     1741 tgcttgcctt gatgcaggag caaaccgctt ctcaattggt atccagtcgt ttaacagcaa
     1801 aatccgcaag aaaatggccc gcacctcgga tggcccaacc gctattgcgt ttatggaaag
     1861 cctggtcaaa cgtgaccgtg ctgcggtggt ctgtgacctg ctgtttggcc tgccgggtca
     1921 ggatgcgcaa acctggggag aagatctggc tattgcccgc gatatcggtc tcgacggcgt
     1981 cgatctctat gcgctcaatg tcctgtccaa tacaccgctg ggcaaggccg tggaaaatgg
     2041 gcgtactacc gtaccctcac cggcagaacg tcgcgatctt tatctgcaag gatgtgattt
     2101 tatggacgat gccggttggc gctgcatcag taacagccac tggggccgta ccacacgcga
     2161 acgcaatctc tataacctgc tgattaaaca aggtgccgat tgtctggcct ttggttccgg
     2221 agccggcggt tcgattaatg gttactcctg gatgaacgaa cgcaatcttc agacctggca
     2281 tgaatccgtc gcggcaggca aaaaaccgct gatgttgatc atgcgtaacg ccgaacgtaa
     2341 tgcgcaatgg cgtcatacct tgcagtcagg tgttgaaaca gcgcgcgtac cgctggatga
     2401 acttacgcca catgccgaaa aactcgcgcc gttactggct caatggcacc aaaaaggctt
     2461 aagtcgcgat gcttcaactt gcctgcggct gactaatgaa ggtcgtttct gggcaagcaa
     2521 tattttgcag tctcttaatg aactaattca ggtacttaat gcgccagcga ttatgcgtga
     2581 aaaaccataa caggagtgat ttatgagcca tgtctcgtta caagaatttt tgaaaacgga
     2641 gccggacggt acgctggagg tggtagccga acaatacaac accacactgc tggaggtagt
     2701 gagaaatcta ccctcctcta ccgtagtgcc aggcgataaa ttcgatactg tctgggatac
     2761 cgtctgcgaa tggggcaacg tcaccacgtt agtacatact gccgatgtaa tcctcgaatt
     2821 tagcggcgaa ctgccttcgg gtttccatcg ccacggctat ttcaacctac gcggaaagca
     2881 cggcatgtcc gggcatatca aagcagaaaa ctgcacgcat attgccttaa tcgaacgtaa
     2941 atttatgggc atggacaccg cgtccattct ctttttcaac aaagaaggca gcgccatgct
     3001 gaaaatcttc ctcggcaggg atgaccatcg tcagctcctg agcgaacaag tcagcgcctt
     3061 ccatactctg gcggcatcgt taaaggaaca cgcctgatga cgccgtggct actctttggc
     3121 gcaggcggaa aaggtgtggg tgccagaacg cttgaactgg cgctggcgga acaacgtccg
     3181 gtcgttgctg tcattcgtca tgccgatgcc gccacgaaac tggcgcaaca aggcgtacag
     3241 gtttttacag gcgacgcctg tgatgccagc gtagtcgcgg ctgcttgccg cgcagctggc
     3301 ccggatgcgc ttatcatctc aacaatggga ggagctcagg attatctggc gcacagaaca
     3361 gtgattgatg aggccgaaaa ggccggcatc agtcgcatga ttctggtgac ctctttggga
     3421 tgcggcgata gctggccgtt tttatcggag cgggcaaagg ctgcgtttgg tcaggcggta
     3481 cgggaaaaaa cgctggcgga aagctggctg caaaccagcc agctagacta cgccattctc
     3541 cgccctggtg gtctgcttga cggcgcagca acaggcaaag cacaacggat acaaaatcag
     3601 gagtgtcatg gttttgtccg ccgtgccgat gtcgccgcac atatccatga actggcaaat
     3661 gcgccagcac ttaaccagca ggtctacagt ctgattgaac cggacctgaa accggcgtga
     3721 taccccggcg cggcatgtcc gcgccgtaat gtcgtaacgg agttttgcgt gctcaaggat
     3781 tcattttcat ccgccagggt ctttatgggg ctatcactat tattgcttgc cctggtgctg
     3841 ttcggtgcta gtcagggagc gttaaagatc agttttgatg ccctttttga tgaggaatac
     3901 cgcgatatct ggctcaatat tcgtctacca cgggttttgc tggcggtgct ggtaggtgca
     3961 gcgttggcaa ccgcaggggt aattatgcag gggctttttc gcaacccaat ggctgaccca
     4021 ggattactcg gcgtcagtag cggttctgca ttaatggttg gcgttgcaat tgtgctgccc
     4081 ttctccttcc cagtggtgct ggtgctctat gagcaaatgg tgtttgccat tgccggaagt
     4141 ttagtagtct gcgccatcat ttttctcatc acgcagcgcc atcgcgatgg cagcatgatg
     4201 caattattac tcgccggtat cgccatcaat gccctgtgcg gcgcagcgat cggcatcctg
     4261 agctatattg gcgatgagca gcagctacga caactcacat tgtggatgat gggcaatctt
     4321 ggacaggcgc aatggccgac gttattggtt gccagttcat tcatccttcc ggccattatc
     4381 gcaacaactt gtctcgccgg aacgctgaat ttactgcagc tcggtgatga agaagcccat
     4441 tacctcggcg tgaacgttaa gcgtaaacgc cagcaattac tattagtgag ctcactgctc
     4501 gttggtgctg ccgtatcggt aagcggcatt atcggcttta ttggcctggt gatcccgcat
     4561 ctgattcgca tgactaccgg agcaaatcac cgctggctca tcccttgttc cgccctcgcc
     4621 ggagcctgtt tattgctgat ggcagacacg cttgcccgca cgctggtaca gccagcagaa
     4681 atgcccgtgg gattattaac cagcctgctt ggtggccctt attttatgtg gttgattctg
     4741 cgaaatcgga ggatcacatg atcagcgccc agaatctggt ttatagcctt caggggcggc
     4801 gtctgactga taatgtctcg ctgacttttc ccggtgggga aattgttgcc attctcggcc
     4861 ctaatggtgc gggaaaatct acgctgcttc gccagttaac cggctatctt caacctgata
     4921 gcggcgaatg tcggttgttt aataagccat taaatgaatg gtcgattact gaactggcga
     4981 aacaccgggc cgtgatgcgt caaaatagcc acatggcgtt tccattcagc gtacaagaag
     5041 ttatccagat gggtcggcat ccgcatcgta ctggtaatca ggataatgaa acagcacaaa
     5101 taatggcgtt atgtgactgt caggcgttag caaaccgcga ctatcggcaa ttatccggtg
     5161 gtgaacaaca gcgcgtgcaa ttagcgcgcc tgctggtgca actctgggaa ccaactccct
     5221 caccgaaatg gttatttctt gatgaaccaa cttcggcgct ggatattcac catcagcagc
     5281 acttgtttcg tttattacgc cagttagttc acgagcggca gttcaatgtc tgctgcatat
     5341 tgcacgatct aaatcttgcc gcgcattatg cagaccgtgt tgtattaatg caaaaaggaa
     5401 aggttatcgc aaatggtaaa ccacaggacg tattgacaca gcaggcgctg acaatgctct
     5461 acggcgcgga tattaccgta cttaaagatc ctgccaacca ttcaccactg atcgtcctcg
     5521 atcattaaaa gcaacaggag taccgcactg gtactcctgc gaataaataa ctatacagat
     5581 tagctaattg aaacgccttt cacccctgcc atacctttta ataatcgcaa cagatcttct
     5641 atcgacgttg tggcatgaag ctggatatca atggcgacca cttcatgatc ttcattttcc
     5701 tgcaacgaaa ctaaatcagt ttttattttt tgctgcttaa accagtcgag cattgatacc
     5761 gtattaccgt tcactaatgt cagctgtaaa tggtaatttt tgttcatcag gcggaaggtt
     5821 aattgatgga agacttccag caccagcaag gtcatcactg aaccataaat acccagttcg
     5881 tacataccac tgccaataac catacctatg gcggcggtca cccagatatc cgctgccgtc
     5941 gtcaaaccta caatattttg gttacgaacg agaatgttac ctgcaccgat aaacccgacg
     6001 cccgtcacca cctgagcagc aatacggctg gggtccagtc cgacgtgatc taaagacagc
     6061 acgtcagcaa aaccatattt cgaaacaatc ataaacagtg cgcttcccat gccaattaat
     6121 acatgagtgc gtaaccctgc tcctttgccg cgcatttgcc tttccatgcc aatagcgcca
     6181 caggcaattg ccgcgagtat aagacgtata ataaattctg ctgtcatata aaaatgcaaa
     6241 aaggagcagc aagatggctc aacttgccac tcctttttac ttgtacctca ttaattcggc
     6301 aagtcattag atgcttgatt tttgaaggta tacaaatttt tctgcgggtt tttcttacag
     6361 tattcgatca ctttaggaat ttgagtgaga tcggtttcat ttaaagtaac tgtatcgcca
     6421 cctttatata ctgtttcttc atgcagcatc caccatgcaa ccggggtcat tgcttttgga
     6481 ttcagatcaa taaattcctg gcaggtcata tctttagcgg attcattggc tgccagcgca
     6541 gattgtgcgt tcaccagtga caaagccgct acagcgccca taaaaataaa cgctttacgg
     6601 agagatgaaa tatttatttt gtaacccatt caatatagag attatatatg aacaggagga
     6661 atagctgctt ttaaagaaga tattccggcc tgttcaatat gcaaggaagt acgatgtaac
     6721 gcattcccgg aattacatat ctttcttaat tttgtcccat tcgcctttaa ctttatcttt
     6781 aaagttggct tgtttatcct gagtacaagc ctgaacgata gctggggtta cggttgcaat
     6841 accctgaaca tctaaaaccg catcttctgg tttatctttg ttgttcagcg cttcagcaaa
     6901 accaactgca gttggctgga aggattcgtc cacagccagg aaatcttcac aggtccagga
     6961 gttgaccggt tttttgttat cagctgcttt ttgcgcatcc gctgcattgc tcacaactgg
     7021 cagaagaagc agaccaccaa gaataacgcc taatactttt ttcatcgtaa tatcctcaac
     7081 tataaagtga aagagccgtc acgaatcaat ttcgacactg aggttataac ctggttttct
     7141 gtatatgtca tgttgatgga aaatatcaaa atcagatatt tttatttcaa tacaa
//
LOCUS       DAZKIP010000186         7064 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZKIP010000186 DAZKIP010000000
VERSION     DAZKIP010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7064
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.188"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..434)
                     /locus_tag="ACHOUK_004427"
     CDS             complement(<1..434)
                     /locus_tag="ACHOUK_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HFP9899742.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLAPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGV"
     gene            complement(691..1326)
                     /gene="msrQ"
                     /locus_tag="ACHOUK_004428"
     CDS             complement(691..1326)
                     /gene="msrQ"
                     /locus_tag="ACHOUK_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFP9899743.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWIILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKSRSLFNRLRKQVHNKLSV"
     gene            complement(1327..2331)
                     /gene="msrP"
                     /locus_tag="ACHOUK_004429"
     CDS             complement(1327..2331)
                     /gene="msrP"
                     /locus_tag="ACHOUK_004429"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFP9899744.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKALEFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(2439..2852)
                     /gene="hiuH"
                     /locus_tag="ACHOUK_004430"
     CDS             complement(2439..2852)
                     /gene="hiuH"
                     /locus_tag="ACHOUK_004430"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFP9899745.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNKHYHVPLLLSQYGYSTYRGS"
     gene            2985..3656
                     /gene="hprR"
                     /locus_tag="ACHOUK_004431"
     CDS             2985..3656
                     /gene="hprR"
                     /locus_tag="ACHOUK_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFP9899746.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3656..5014
                     /gene="hprS"
                     /locus_tag="ACHOUK_004432"
     CDS             3656..5014
                     /gene="hprS"
                     /locus_tag="ACHOUK_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFP9899747.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDLLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNSYLNIDIASPGAK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRITLPQ
                     RN"
     gene            complement(5122..5973)
                     /gene="hchA"
                     /locus_tag="ACHOUK_004433"
     CDS             complement(5122..5973)
                     /gene="hchA"
                     /locus_tag="ACHOUK_004433"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFP9899748.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPQKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            complement(6565..>7064)
                     /locus_tag="ACHOUK_004434"
     CDS             complement(6565..>7064)
                     /locus_tag="ACHOUK_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFP9899749.1"
                     /translation="VHKGTHNTRYGDRFDATAGGETAVAWTVGAKYDANNVYLAAMYA
                     EPRNMTGYGDADAIANKTQNFEVVAQYQFDFGLRPSIAYLQSKGKDLGGVNSDNFDSQ
                     GNHHYTNKDLVKYVDIGMTYYFNKNMSTYVDYKINLLDNDDDFYKENGIATDDIVAVG
                     LVYQF"
BASE COUNT         1932 a         1473 c         1686 g         1973 t
ORIGIN      
        1 acgcctttct tgcctgattt ataggtgagt attttgtatc catcgtaatc atatttacag
       61 gatgttgttt cattatttct atggaattca acaatgccgt cctcaatgcc aatcatctcg
      121 atatctgttg catagccttt gtgataataa tctttaattt cagcaaatgt tttagtttta
      181 tctgcatccg ctttcttctg aaagacgggg gcaagtttgc cactttgcag taaaggataa
      241 acggattgcc agactccatc ccagtcactg agcgttcggt tttgtacatt ggcatcatca
      301 aaaacaccat tagcagcttt ttgttcgacc tctgttaagg gtttgccgtg tgagtgatga
      361 ccatgcgaaa aggcaggagc gctaacaata aagacaccta aagcaacagc cagtttgtga
      421 agacgaatcg ccaaaacagt ttcctccaga gtcattgtat atgatgtgta atgttatatt
      481 gtaacatatg acaaaatgca acaactctta ggagatgaga gcatagtttt ctcaggtgta
      541 cttatctatc ttattgaaat aaaatgaatt atgttgataa tttgaggttg atgacaaacg
      601 atacataacc tgatgtgatc tgcgtaggcc ggatagggcg ctcgcgccac atccggcagg
      661 gataacgcgt actttgtcgg caatatgaag ttatacagat aatttgttgt gaacctgctt
      721 gcgtaaccgg ttaaaaaggg aacgcgactt cttataccgt aaggctaaaa gcagtacagc
      781 cagcccagcg tagatgagcg gctgcggtga aataatcttc actgaccaca gataatgtat
      841 tggggccagg atcgcgacaa gatagacgaa attatgcaac tgttgccagt gcttgccgag
      901 ttttcgctgc atcgcctggg ttgaagtaaa cgctaaagca agcaaaataa tccagctgat
      961 aattcctaac gttaaataag gccgggtaat taactccttg cctaataacg ccagattatt
     1021 cacgcccagc tccagcaatg cgtaactggt taaatgcagt gtcgcccagg caaagcacca
     1081 taagcctaac aggcggcgtg tgcgtatcaa taacggctgt tttgcgtagc gtgccagagg
     1141 tgtgattaac aaggtcgcca gcaaaaattt cagtgcagtg cgaccggtaa aatgctgaat
     1201 atctttcacc ggatcggcac ccagtccacc gtgattgatc gcccagacca gccagagaaa
     1261 cggcaacaat ccggcaagat gcaggcaaac tttcagccat gtcacctgtt ttgctgtcag
     1321 acgcattcag aaattctccc gcaaatccag gccacgatac agcgatgcca cctggtcggc
     1381 gtaaccatta aacagtagcg ttggctggcg ctgtacatcg aggatgccgc ctgaaccaat
     1441 aaatcgttcg gtagcctgtg accagcgtgg gtgatcaaca tgcggattaa cgttggcgta
     1501 aaaaccgtat tcgtcaggcg ctgccagatt ccaggtggtt ggcggacgct cgcgggtcag
     1561 cttaatactg acgatcgatt taatcccttt aaagccatat ttccacggca caatcagtcg
     1621 caccggcgcg ccattttgtg gcggtaacgc cttgccataa acacctacgg tcatcagtgt
     1681 gagcggatgc attgcttcgt cgagacgcaa tccttcgaca taaggatatt tcagcccgcc
     1741 gccgataaag cggtcctgct ggcctggcat ctgttccggt gcataaattg tttcgaaagc
     1801 gacatacttc gcattgctgg tgggttcggc aagcgccagc aatttgtgca gcggaaaacc
     1861 aatccacggc accaccatcg accacgcttc cacgcagcgc atacgataaa tacgctcttc
     1921 cagcgggaag cgacgggtta aatcatcgtg atcgagggtc aatggttttg ccacttcgcc
     1981 gctgattttc agtgtccatg gatcggtttt caggctacca gcattagcgg cgggatcggc
     2041 tttatccagc ccgaattcat agaagttgtt ataaccggag actttatctg ctggcgtcag
     2101 tggcaggtta ttttgccagg cggcaggctt gctgaactcc agcgcttttc cggcgggggc
     2161 gggtgggcga tcgttccctt taaaccagct aagcagatcg gcatgcgcag cgtgaggcaa
     2221 agaaagtgca gctgcgctga tgcccagtgc ttttaacacc tgccgacgct tcataaagaa
     2281 taccgactcg gccgtaacat ctgattcttt taaaaattga ttctttttca taagccatca
     2341 cactttttgt cgttagccag caagagccgt ttactgttaa tctgtcaggg agtgtcctcc
     2401 ctaacagtag cagagatctg cttttacata tttatgcatt aactgccacg ataggttgaa
     2461 tacccatatt ggctaagtaa taaaggcaca tgataatgct tgttcacttt attaatatga
     2521 aattcaacgg ggatctcagg gaagaaactt tcaagatttt gtttcttgaa atagtccccg
     2581 gttttaaata cgacacggta atcgcccgta gttgcagttt gctcgggcca cagtgcctta
     2641 attcgtccat ccttatctgt tttggcggta ttaagttgta accagccgtt gtccgctttc
     2701 ttttcaagag tgactgtcac gtcggcagcg ggttttcctg tttgctggtt caaaatgtgc
     2761 acgctaagaa tgttttgttg tgcagcatag accaaagaag gtaatgaaaa tgcagccgtt
     2821 actacggaga gtactaaata acgctttaac atgtttatat ccttgtcatg tgaatgagtt
     2881 ttgaggatag cgaaaaagcg tccagggtaa ccttacagca gcattacaat tttgtaatga
     2941 accaggctgt ttctatgaca tatgatttat ggcatattat tttcatgaag attttactta
     3001 ttgaagataa tcaaaggacc caggagtggg taacgcaggg gctttccgaa gcgggttatg
     3061 tcattgatgc cgtttctgat ggcagagatg ggctttatct tgcgctgaag gatgattatg
     3121 cattgatcat tctggatatt atgcttccgg gtatggatgg ctggcagatc ttacaaacgt
     3181 taagaacggc aaagcaaacc cctgttattt gccttactgc aagggattct gtcgatgaca
     3241 gagtcagagg gctggacagt ggagcaaatg attatctggt aaaacctttt tcattttctg
     3301 agttgctggc aagggttcgg gcacaattaa ggcaacatca cgctttgaat tcaacattag
     3361 aaatcagcgg cttaagaatg gactctgtta gtcaaagtgt gagcagggac aatatcagta
     3421 ttacactgac gcgcaaggag tttcagttac tttggctact ggcctccaga gctggcgaaa
     3481 ttatacccag aacggttatt gcgagtgaaa tttggggaat caactttgat agtgatacca
     3541 atacggtgga tgtcgccatt cgcaggctcc gcgcaaaagt tgatgatcct tttcctgaaa
     3601 agctaattgc cacaatccgt gggatgggat attcgttcgt agcggtaaaa aaataatgaa
     3661 aagattatct ataaccgtcc gtttaacctt gctttttata ttgctgctgt ctgttgctgg
     3721 cgccggaatt gtctggactc tctataatgg cctcgcaagt gagttgaaat ggcgcgatga
     3781 tacaacactc attaaccgga cagcgcagat caagcagtta ttaattgatg gggtaaatcc
     3841 agatacgtta cctgtatact ttaaccggat gatggatgtt agtcaggata tcttgatcat
     3901 tcatggtgat ggcatcaata aaattgttaa ccggacaaat gtcagtgatg acatgttaaa
     3961 taacatacct gctagtgaga caatcagcgc agctggcatt tacagaagca ttattaatga
     4021 tacagagata gatgctttac gaattaatat tgatgaagtt tcgccatcat taacggttac
     4081 tgtggctaaa ttggcttcag ccagacataa catgcttgaa cagtataaaa tcaatagcat
     4141 tataatttgc attgtcgcca ttatactgtg ctcagtatta agtccgctgt taatccgaac
     4201 ggggttacga gagatcaaaa agttgagtgg tgtaacggaa gcgctgaatt ataacgatag
     4261 ccgggagcct gttgaggtta gcgcattacc gagagaacta aaacctcttg ggcaggcgtt
     4321 gaataaaatg catcatgctt tagtcaaaga ttttgagcgt ctaagtcagt ttgctgacga
     4381 tctcgctcat gaacttagaa cgccaattaa tgcattactg ggtcagaatc aggttacgct
     4441 cagtcaaacc agaagtatcg ctgaatatca aaaaacaatt gccggtaaca ttgaagagct
     4501 ggaaaatatt tcgcggttaa cagagaacat actgtttctt gccagggcag ataaaaacaa
     4561 tgttttggtg aaactggact tgctttctct caataaggaa gtcgaaaatt tgttggacta
     4621 tcttgaatat ctttcagacg agaaagagat ttgctttaag gtcgagtgca atcagcaaat
     4681 ctttgcggat aaaattttac tgcaacgaat gttatcgaat cttattgtta acgctattcg
     4741 atattcgcca gaaaaatcgc gtattcatat aaccagtttt cttgatacca acagctatct
     4801 taatattgat atcgccagcc ctggagcgaa aattaatgag cctgaaaaac tcttccgtag
     4861 attttggcgg ggagataatt cgcgtcattc cgtaggtcag ggacttggtc tttctttagt
     4921 caaagcgatt gccgaattgc atgggggaag tgctacgtat cactatctca ataagcataa
     4981 tgtgttccgg attacgttgc cgcaaagaaa ttaacatagg gcttcagtac gccataaatt
     5041 aaccgtgaaa aaacgcagcc acgtcgtgag tactaacgcg gctgcgattg attatgcgct
     5101 tacattcaaa cgtaacaggg attaacccgc gtaagctgcc agcatttcct gcgccgccag
     5161 tttacccaac gcattcgctg caaaaggact gtcgccggtg agaagtttac ggtccttatg
     5221 tactcgcccg gtgatgtcgt cattaatgat gttcatgccc attttcttca gttcttcgcc
     5281 gaagtaccag gtgagatgac ccggcatata gccaatctct ggcgtttgtt tgtctgcggc
     5341 gtctgggaat gcgcaaatgg aataaccatt cagtggatta tcgccgtggc gaagcgccag
     5401 aaaagccgcc gggccgtggc aaagggagat aacaaaacgg tcatttttga ttgcccactg
     5461 taaagcggca gctacgtcct ggctttcagg taaaccaata agtgcgccat gaccaccagg
     5521 aacaaagatt gctgcatatt cgctatcagc gttgaggctg gcgacaacat ccgcgagttt
     5581 cttcggattg cggaacaacg atttatgctg ctcaaagaat ggcattactt tttcatcttt
     5641 ctgcggcata gcccagtatt caaacttggt catcagaccg gaaatggtcg ccacttcgaa
     5701 ttcgaaacct gcagcatgga gatgatacaa cggcagcaac gtttcaatcg gatggttacc
     5761 ggtcgagaac agttttccgt tatcggtcgg caaataacgt tcgtccgcgg cgatcaccag
     5821 aattttatgt ttaccgcgat aaggttttgg atagtccacg ccatcaagat cagagacagg
     5881 actggtatat tggctaagcg aatattctga agggaagaat gcattatctt cagcaatatc
     5941 gacctgcgga tttttactgg tttgaacagt catagtgtat tccttattgt tgcttagtta
     6001 gggtagtcac tatatttgcg actgagctgg tcgctatttt taaccaggct ggaaaattga
     6061 gtcaatatat cgaaagcggt agtcgacggg tgtaagtatg taatgatgat gctgcactgg
     6121 tgttgaatat tattcacgcc aaattttcct tattgagcaa cttcgtcgca agtggcctca
     6181 atgattttag accattatca gaggtacggg gcggcgggga gagatttagt gctgtggaat
     6241 attgtggtgc tgatgaatat ttatatcgcg gattggtaga gaaaaagctt atttcatgca
     6301 ctatttttgc aaatatttct atatgggaag cgtgtttgtt acggattaat tcataaagaa
     6361 atgcatttgg gtgttggctt gaagagtcgc tggttgatgt tattgtgcca ggaggggcag
     6421 ggtaaggggg tgtatcgata agctttggca attgcgtgtg ccgcagatgg agatgaaatg
     6481 ccaataaaaa agcctgagtt cgatgaggga cgaactcagg ctagtgttca gttttacaat
     6541 aacacgccgc ggcgcgttgg ctgattagaa ctggtagact aagcccacag ccacgatatc
     6601 atcggtagcg atgccgtttt ctttgtagaa gtcgtcgtcg ttgtccagca ggttgatttt
     6661 gtaatcaaca taagtggaca tgtttttgtt gaagtagtag gtcataccaa tatcaacata
     6721 tttaaccaga tctttgttgg tgtaatgatg attaccctgg ctgtcgaagt tatcactatt
     6781 tacaccgcct aaatccttac ctttagattg cagataagcg atggacgggc gcagaccgaa
     6841 gtcgaactga tactgagcaa caacttcaaa gttttgggtt ttgttagcga tggcatcagc
     6901 atcaccgtaa ccagtcatgt tacggggttc agcatacata gctgctaagt aaacgttatt
     6961 agcgtcgtat ttagcaccaa cggtccatgc aactgctgtt tcaccgccag cagttgcatc
     7021 gaagcgatct ccataacgag tgttatgagt acctttgtga acct
//
LOCUS       DAZKIP010000187         6958 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZKIP010000187 DAZKIP010000000
VERSION     DAZKIP010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.189"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            217..342
                     /locus_tag="ACHOUK_004435"
                     /pseudo
     CDS             217..342
                     /locus_tag="ACHOUK_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
     gene            407..667
                     /locus_tag="ACHOUK_004436"
                     /pseudo
     CDS             407..667
                     /locus_tag="ACHOUK_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            717..956
                     /locus_tag="ACHOUK_004437"
                     /pseudo
     CDS             717..956
                     /locus_tag="ACHOUK_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(1004..1621)
                     /gene="ureG"
                     /locus_tag="ACHOUK_004438"
     CDS             complement(1004..1621)
                     /gene="ureG"
                     /locus_tag="ACHOUK_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HFP9899750.1"
                     /translation="MNIIKQPLRVGVGGPVGSGKTALLEALCKSMRDTWQLAVVTNDI
                     YTREDQRILTEAGALEAERIVGVETGGCPHTAIREDASMNLAAVEALSEKFGNLELIF
                     VESGGDNLSATFSPELADLTIYVIDVAEGEKIPRKGGPGITKSDFLVINKTDLAPYVG
                     ASLEVMERDTLRMRGERPWGFTNLKSGEGLQNIIAFIEEQGMLGK"
     gene            complement(1633..2307)
                     /locus_tag="ACHOUK_004439"
     CDS             complement(1633..2307)
                     /locus_tag="ACHOUK_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HFP9899751.1"
                     /translation="MPTPEKRLRLMQLASNSLPVGGYSWSQGLEWAVEAGWVEDSAAF
                     EHWQQLQMEQSFFAVDLPLLARLYRACEAGDPDSAGRWTAYLLACRETRELRDEERNR
                     GAAFTRLLVDWQPDCPAEWRKLCQQSQLTGMAWLGVRWQISVSDLALSLGYSWIESAV
                     MAGVRLVPYGQLAAQQLIMRLCARYAANMDSALATPDHAIGSATPLASIASARHETQY
                     SRLFRS"
     gene            complement(2308..2772)
                     /gene="ureE"
                     /locus_tag="ACHOUK_004440"
     CDS             complement(2308..2772)
                     /gene="ureE"
                     /locus_tag="ACHOUK_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HFP9899752.1"
                     /translation="MLYLTRRVETPAQTTASVTLPVDMRVKSRIKVTLNDGRQAGLLL
                     PRGLLLRDGDILSNENGDEFIKVIAADEAVSVVRCADPFMLAKACWHLGNRHVPLQIM
                     PGELRYHHDHVLDDMLRQFGLDVDFAHLPFEPEAGAYASKSHAHNHDQEHSH"
     gene            complement(2782..4485)
                     /gene="ureC"
                     /locus_tag="ACHOUK_004441"
     CDS             complement(2782..4485)
                     /gene="ureC"
                     /locus_tag="ACHOUK_004441"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HFP9899753.1"
                     /translation="MSNISRQAYADMFGPTTGDKIRLADTELWIEVEDDLTTYGEEVK
                     FGGGKVIRDGMGQGQMLSAGCADLVLTNALIIDYWGIVKADIGVKDGRIFAIGKAGNP
                     DIQPNVTIPIGVSTEIIAAEGRIVTAGGVDTHIHWICPQQAEEALTSGITTMIGGGTG
                     PTAGSNATTCTPGPWYIYQMLQAADSLPVNIGLLGKGNCSNPDALREQVAAGVIGLKI
                     HEDWGATPAVINCALTVADEMDVQVALHSDTLNESGFVEDTLTAIGGRTIHTFHTEGA
                     GGGHAPDIITACAHPNILPSSTNPTLPYTVNTIDEHLDMLMVCHHLDPDIAEDVAFAE
                     SRIRQETIAAEDVLHDLGAFSLTSSDSQAMGRVGEVVLRTWQVAHRMKVQRGPLPEES
                     GDNDNVRVKRYIAKYTINPALTHGIAHEVGSIEVGKLADLVLWSPAFFGVKPATIVKG
                     GMIAMAPMGDINGSIPTPQPVHYRPMFAALGSARHRCRVTFLSQAAAANGVAEQLNLH
                     STTAVVKGCRTVQKADMRHNSLLPDITVDSQTYEVRINGELITSEPADILPMAQRYFL
                     F"
     gene            complement(4478..4798)
                     /locus_tag="ACHOUK_004442"
     CDS             complement(4478..4798)
                     /locus_tag="ACHOUK_004442"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HFP9899754.1"
                     /translation="MIPGEYKVKPGYIELNIGRATCSIIVENHGDRPIQVGSHYHFAE
                     VNPALKFDRQKARGYRLNIAAGTAVRFEPGQKREVELVALSGARIVHGFRGDIMGELE
                     ANDE"
     gene            complement(4807..5109)
                     /locus_tag="ACHOUK_004443"
     CDS             complement(4807..5109)
                     /locus_tag="ACHOUK_004443"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HFP9899755.1"
                     /translation="MELTPREKDKLLLFTAALLAERRLARGLKLNYPESVALISAFIM
                     EGARDGKSVAALMEEGRHVLSREQVMEGIPEMIPDIQVEATFPDGSKLVTVHNPII"
     gene            complement(5200..5898)
                     /locus_tag="ACHOUK_004444"
     CDS             complement(5200..5898)
                     /locus_tag="ACHOUK_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HFP9899756.1"
                     /translation="MLDLRFQRLHGKTTLTTRHHVGLLTVQRPFYPEEETCHLCLLHP
                     PGGIVGGDELIINAIIDSDCHTLITMPGASKFYRSSGAQAHLQQNLTLCNNATLEWLP
                     QDSIFFPGAHATLHTVFHLSSSSTLLAWDLLCLGRPVIGETFSHGTLANRLEIWVDGS
                     PLLIERLHVANGELTCVARKPWVGTMLFYLGNETQLEDIREKLTPLENYAGATLTDGL
                     LTVRFLSDDNLRCQ"
     gene            6279..6554
                     /locus_tag="ACHOUK_004445"
     CDS             6279..6554
                     /locus_tag="ACHOUK_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899757.1"
                     /translation="MTEHWSGHADDNFPFSWGSDSIFMHACLQSYGESICDTVHTFVA
                     DVAPESLTGLQTVYLYTAGLLCDIVFHIEGGPPEMNGIIIFPDDIIP"
     gene            6779..>6958
                     /locus_tag="ACHOUK_004446"
     CDS             6779..>6958
                     /locus_tag="ACHOUK_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFP9899758.1"
                     /translation="MIKKISINFLFLMLMIDVVFATLFNIPVWMHLFNIINNLDGVKL
                     GFIISLPVFLISALNF"
BASE COUNT         1665 a         1732 c         1792 g         1769 t
ORIGIN      
        1 taacgcgctc acataaatca tcatctgata atataacaac acccattatt ttattctgac
       61 ctaaaaactt cttatgaaat aaacgtttac ccgacgtatc tgatcccgct gtttttacct
      121 gtatccttta acatacttga gaagcccgct gaaaaaatat accgtaaagg cggtttctct
      181 gtcttccata aacctgtcgg ataagaaggg cattaaatga aaaccaaaaa agttatgatg
      241 attgcactgg tcagctctgc gctggctctt tcaggatgtg gagccatggg cacagcgatc
      301 aaaaaacgaa atctggaagt aaaaacccag atgagtgaaa cctgaggtag cctgagttta
      361 acggacactc cttcctgaaa tagaatggca tcagaaggag ctaataatga gcagaaaaac
      421 ccaacgttac tctaaagagt tcaaagccga agctgtcaga acggttcttg aaaatcaact
      481 ttcgatcagt gaaggcgctt cccgattatc tcttcctgaa ggcactttag gacaatgggt
      541 taccgccgcc agaaaagggc tcggtactcc tggttcccgc acggtggctg aactggaatc
      601 tgaaattctg caactgcgta aggcgttaaa tgaagctcgc cttgagcgag atatattaaa
      661 aaaagcagct gtagattcaa tctgtcaatg caacacccct ttcaattatc tctttcactg
      721 cccgcgacgg tatcggttat cagtttgacc tgctggctaa cggtgaaacc gtactgcgtg
      781 atgcatccgg cgaaaaacgc atcaagggtg aaaaagacgg tcgtatgctc atgaatgttg
      841 gcctgaatgc ggaaattcgc gacaacatcc gcttcggtct ggagtttgaa aaatcggcat
      901 ctggtaagta caacgtggat aacgcggtga acgccaactt ccgttattcc ttctgataaa
      961 aacaaggggc cgcctgccgg ccctgataca gaggatgttg tgattactta cccagcatgc
     1021 cctgctcttc gataaaggcg atgatgtttt gcagaccttc accacttttc aggttagtga
     1081 acccccatgg gcgttctccg cgcatacgta aagtatcacg ctccattacc tcgagggagg
     1141 cgcccacgta tggtgcaagg tcagttttat tgatcacgag gaagtcggat ttggtgatcc
     1201 ccggaccgcc tttacgcggg atcttttctc cttctgccac atctatgaca tatatggtaa
     1261 ggtccgccag ttcggggctg aaggtcgcac tcaggttgtc gccgccgctt tccacaaaga
     1321 tcagctcgag attaccaaat ttctcactca gtgcttccac ggctgccaga ttcattgatg
     1381 cgtcttcacg aatggctgta tgagggcagc cccccgtttc gacaccgaca atgcgttccg
     1441 cttccagtgc tccggcctca gtgagaatgc gttgatcttc tctggtgtag atgtcgttgg
     1501 tgactacggc taattgccag gtgtcacgca tggatttgca gagggcttcc agaagcgcgg
     1561 ttttacctga gccgaccgga ccgccgacgc cgacacgcag cggttgcttg attatgttca
     1621 ttttattttc tcctaagaac ggaataatcg ggaatactgg gtttcatgtc gcgcggaagc
     1681 gatagatgca agtggcgttg ctgaaccaat tgcgtgatcc ggggttgcaa gggcactgtc
     1741 catgttcgcc gcatagcggg cacacaggcg cataattaac tgctgggcag ccagctggcc
     1801 gtagggtacc agcctgacgc ccgccatcac tgcgctttca atccagctgt agccaaggct
     1861 cagggccagg tctgaaacgg atatttgcca gcgtacgccg agccatgcca taccggttaa
     1921 ctggctttgc tggcatagtt tgcgccattc ggccgggcag tcgggctgcc agtcgaccag
     1981 aagtctggtg aaggctgcac cgcggttacg ttcttcatcg cgtagctcac gggtttccct
     2041 gcaggccagc agataagcgg tccagcgccc ggcactgtca ggatcgcccg cttcacaggc
     2101 ccggtatagc cgggcaagca gtggcaggtc gacggcaaaa aaactctgtt ccatctgcaa
     2161 ctgctgccag tgttcaaagg cggcgctatc ttctacccag cctgcttcca ctgcccactc
     2221 cagtccctgg gaccagctgt agcctccaac agggaggctg ttactggcaa gctgcatcag
     2281 ccgcagacgt ttttccggag tcggcattca gtggctgtgc tcctgatcgt gattatgggc
     2341 gtgcgacttg ctggcgtaag cgccggcttc cggctcaaac ggaagatgtg caaaatcaac
     2401 atccagccca aactggcgca gcatatcatc gaggacgtga tcgtgatggt agcgcagctc
     2461 tccgggcatt atttgcagcg gaacgtggcg attgccgaga tgccagcagg ctttcgcgag
     2521 cataaaagga tctgcgcagc gcactacaga aacggcttca tcagcggcga tcaccttaat
     2581 gaattcatca ccgttttcgt tactgagaat atcaccatcg cgcagcagca gaccgcgtgg
     2641 cagcaacagt ccagcctgac gtccatcgtt aagggtgact ttaatacggc tttttacccg
     2701 catgtcaaca gggagggtaa cgctggcagt tgtctgagcc ggggtctcca cccgccgggt
     2761 caaataaagc atttgtgctc cttaaaacag gaaataacgt tgcgccattg gcagaatgtc
     2821 cgccggttca ctggttatca gttcgccgtt gatacgcact tcgtaggttt gtgaatccac
     2881 ggttatatca ggcaacaggc tgttgtggcg catatcggct ttttgtactg tgcggcagcc
     2941 ttttaccaca gcagttgtgc tgtgcaggtt aagctgttca gcgacgccat ttgctgctgc
     3001 tgcctgcgac aggaaagtca cacgacagcg gtgacgggca ctgcccaatg cagcgaacat
     3061 tgggcgatag tgcaccggct gcggtgtggg gatagagccg ttgatatcac ccatcggcgc
     3121 catggctatc attccgcctt tgacgatagt cgccggtttt acgccaaaga acgccgggga
     3181 ccagagcacc agatccgcca gttttcccac ttcaatcgag ccgacttcat gagcaatacc
     3241 gtgggttaat gccggattaa tggtgtattt agcgatatag cgcttcacgc ggacgttgtc
     3301 gttatcacca ctttcttccg gtaacgggcc gcgctgaact ttcatccggt gtgccacctg
     3361 ccaggttcgt aacactactt ctccgacgcg tcccatggcc tgcgaatcgg acgaggtgag
     3421 ggagaacgcg ccaaggtcgt gcaggacgtc ttccgcggca atggtttcct ggcgaatgcg
     3481 cgattcggca aaggctacgt cctcggcgat atccgggtca agatgatggc aaaccatcag
     3541 catgtccaga tgctcatcaa tagtgttgac ggtatagggt agcgtcggat tggttgagga
     3601 aggcagaata ttggggtgcg cgcaggcggt gataatatcc ggagcatgac caccacctgc
     3661 accttctgta tggaaggtat ggatagtgcg cccgccgatg gcagtcagag tatcctcaac
     3721 gaatcctgat tcgttaagcg tatcactgtg tagcgcaacc tgaacgtcca tttcgtcggc
     3781 tacagtcagt gcgcagttga ttaccgcagg tgtagctccc cagtcttcgt gaattttgag
     3841 gccgataacc ccggccgcga cctgctcacg aagcgcatcc ggattggagc aattgccttt
     3901 acccagcaac ccgatattga ccggcaggct gtctgcagcc tgcagcattt gataaatgta
     3961 ccatggtcct ggggtacagg ttgtggcgtt agaacccgct gtcgggccag taccgccacc
     4021 gatcatggtg gtaatgccgg atgtcagcgc ttcttcagcc tgctgtgggc agatccagtg
     4081 aatatgcgta tcgacgccac ctgcggtaac gatcctgcct tctgcggcaa taatttccgt
     4141 ggatacgccg attgggatag tgacgttggg ttgtatatca ggattaccgg ctttgccgat
     4201 agcaaatatc cttccatctt tgacgccgat atcggcttta acgatccccc agtaatcgat
     4261 gatcagggca ttggtcagca ccagatcagc gcagccggcg gagagcattt gcccctgtcc
     4321 cataccgtcg cggattactt taccgccgcc gaatttgacc tcttcgccgt aggtagttaa
     4381 atcatcttcg acctcgatcc acagctcagt gtctgccaga cgaattttat caccggtggt
     4441 agggccgaac atgtcagcat aggcctggcg tgaaatatta ctcatcattt gcctccagct
     4501 cacccattat atctccgcgg aaaccgtgca cgatacgggc accgctcagc gccaccagtt
     4561 caacctcacg cttttggcct ggttcgaagc gcactgcagt tccggcggcg atattgagac
     4621 gatagcctct tgccttctgg cgatcgaact tcagtgccgg gttaacctcc gcgaagtgat
     4681 aatgggaacc gacctgaatt ggtcggtctc cgtgattttc aacgataata ctgcaggtag
     4741 cccggccgat gttaagctct atatagccag gtttaacctt atattctcca gggatcatct
     4801 gttacctcag attatcggat tatggacggt aaccagcttg gagccgtccg gaaaggtggc
     4861 ttcgacctgg atatcgggga tcatttctgg tatgccttcc atgacctgct cgcgactcag
     4921 gacatgccgt ccttcttcca tcagcgcagc gacgcttttg ccgtcgcgag cgccctccat
     4981 tataaaagcg ctaatcaggg ccacggattc gggatagtta agtttcaggc cgcgggccag
     5041 acgacgctct gccagcagtg cagcggtaaa cagtaatagt ttgtcttttt ctcttggagt
     5101 cagttccata gcgttctctt aagtcagcca gatccgggga agtaccggtg atttgtcagt
     5161 cagttgcggc cgcataaatt gccagatatc acgcattacc tactggcaac gcagattatc
     5221 atcacttaaa aagcgaactg tcagtagacc atcggtgagc gtggctccgg cgtagttttc
     5281 cagtggtgtg agtttttcgc gtatgtcttc cagttgcgtt tcattcccca ggtagaacag
     5341 catcgttccc acccatggtt tccgggcaac gcaggtaagt tctccattgg ccacgtgcag
     5401 acgctcaatc aacagcggag aaccgtcgac ccatatctcc agacggttag cgagtgttcc
     5461 gtggctgaag gtttctccga tgaccggacg tccaaggcac agaagatccc aggccagcag
     5521 tgtactggag gacgacagat gaaacacggt atgcaacgtg gcgtgagcgc cgggaaaaaa
     5581 aatcgaatcc tgtggcaacc actccagcgt tgcgttattg cacagcgtca ggttttgctg
     5641 aaggtgcgcc tgcgcgccgc tgctgcggta aaatttactg gcaccaggca tggtgatcag
     5701 cgtatggcaa tcagagtcaa ttattgcgtt gatgatgagt tcgtcaccgc cgacgatacc
     5761 accgggtgga tgaagcagac agaggtggca ggtttcttct tcgggataaa aaggacgctg
     5821 aacagtgagc agaccgacat gatgacgggt ggtgagcgtg gttttcccgt gcagacgctg
     5881 aaaacggaga tccagcatgg cctgccagcc ttttttattt tgtggtaacg cggtactgag
     5941 caatcttacg cccctgataa ataaagataa aacgacagta aagagccgga tttccttgtt
     6001 atataaaaga tgatgccttt ccagtgagtt ttcgtctgaa taatacgaat gtttgttttc
     6061 gtcatgaagc gttccggctc ccggggctat acatcgccag aaaagcaatg catattgtcg
     6121 ggattatcca gagaatcagg cagaaagatc cgacaatggg ctgtgattat gccatctggc
     6181 tcagaatgat atcaaggtgt tatggggata acttgctgat acaatagcag tatgacagat
     6241 tcattaatgc ggaaactgct cactgaaaca gagtgttcat gactgagcac tggtcgggtc
     6301 atgcagatga taatttcccc ttttcttggg gaagcgacag tattttcatg catgcctgtc
     6361 ttcagtcata cggggagagc atctgtgata cagtccatac atttgttgcg gacgtcgctc
     6421 ctgagtcact gactggtctg caaacagttt atctgtacac ggcagggctt ctgtgtgata
     6481 tcgtttttca tatcgagggg gggcctccag agatgaatgg aataataata tttcctgatg
     6541 atataatacc ttagagaatt tctgtgtatg ctattgtgaa catatttttt attaaaaaga
     6601 tgttagtggt gttgtaaaat aaaagaatca gtaactataa acgcctttat tgacataaag
     6661 ttttgatgat gaagttgaag tttgacctgg tataaatcag tattcacttt accaggtttt
     6721 ataaaaatat atcactttta gatacggaca attataggct ctaattaatg ggggtaagat
     6781 gattaaaaaa atcagtatta attttctttt cttgatgtta atgatagatg ttgtttttgc
     6841 cacattgttc aatatccctg tctggatgca tctgtttaat attattaata accttgatgg
     6901 tgtgaaactt ggatttatta tatctcttcc agtctttctg atatctgcat taaatttt
//
LOCUS       DAZKIP010000188         6924 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZKIP010000188 DAZKIP010000000
VERSION     DAZKIP010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6924
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.190"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            417..1406
                     /gene="araF"
                     /locus_tag="ACHOUK_004447"
     CDS             417..1406
                     /gene="araF"
                     /locus_tag="ACHOUK_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFP9899759.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITSNELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKGVEPTKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1476..2990
                     /gene="araG"
                     /locus_tag="ACHOUK_004448"
     CDS             1476..2990
                     /gene="araG"
                     /locus_tag="ACHOUK_004448"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFP9899760.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3005..3991
                     /gene="araH"
                     /locus_tag="ACHOUK_004449"
     CDS             3005..3991
                     /gene="araH"
                     /locus_tag="ACHOUK_004449"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFP9899761.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVA
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4158..4958
                     /gene="otsB"
                     /locus_tag="ACHOUK_004450"
     CDS             4158..4958
                     /gene="otsB"
                     /locus_tag="ACHOUK_004450"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFP9899762.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDINVQLHTVIAQYPGAELETKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            4933..6357
                     /gene="otsA"
                     /locus_tag="ACHOUK_004451"
     CDS             4933..6357
                     /gene="otsA"
                     /locus_tag="ACHOUK_004451"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFP9899763.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6364..6792)
                     /gene="uspC"
                     /locus_tag="ACHOUK_004452"
     CDS             complement(6364..6792)
                     /gene="uspC"
                     /locus_tag="ACHOUK_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFP9899764.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCRKYH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT         1742 a         1624 c         1805 g         1753 t
ORIGIN      
        1 gacgaaatga atgacgtgca ttttccacat ctttgagttg cggttattac accatttcaa
       61 aaactcaacg ccaggtaatg cggcctattg actggtgaaa aagaagacat cccgcatggg
      121 taccaaagac aacaaggatt tccaggctaa tcttatggat taatctgctg tgcattcaac
      181 aatttgtctg acaaattggc ttttccttat gtcttttccc gctaaattta tgcacgttct
      241 cactgtaatt ctgcgatgtg atattgctct cctatggaga attaatttct cgctaaaact
      301 atgtcaacac agtcacttat cttttagtta aaaggtaatg ctttgttttc cgattaattt
      361 aacgaatgtc attcgttttt gccctgcaca aaacgacact aaagctggag agaaccatgc
      421 acaaatttac taaagccctg gcagccattg gtctggcagc cgttatgtca caatccgcta
      481 tggcggagaa cctgaagctc ggtttcctgg tgaagcaacc ggaagagccg tggttccaga
      541 ccgaatggaa gtttgccgat aaagccggga aggatttagg gtttgaggtt attaagattg
      601 ccgtgccgga tggcgaaaaa acattgaacg cgatcgatag cctggccgcc agtggcgcaa
      661 aaggtttcgt tatttgtact ccggacccga aactcggctc tgccatcgtg gcgaaagcgc
      721 gtggctacga tatgaaagtc attgccgtgg atgaccagtt tgttaacgcc aaaggtaagc
      781 caatggatac cgttcctctg gtgatgatgg cggcgaccaa aattggcgaa cgtcagggcc
      841 aggaactgta taaagagatg cagaaacgtg gctgggatgt caaagaaagc gcggtgatgg
      901 cgattacctc caacgaactg gatacggctc gtcgccgtac tacgggttct atggatgcgc
      961 tgaaagctgc aggattcccg gaaaaacaaa tttatcaggt acctaccaaa tctaacgaca
     1021 tccccggggc atttgacgct gccaactcaa tgctggttca acatccggaa gttaaacatt
     1081 ggctgatcgt cggtatgaac gacagcaccg tgctgggcgg cgtacgcgcg acggaaggtc
     1141 agggctttaa agcggccgat atcatcggta tcggtattaa cggtgtggat gcggtgagcg
     1201 aactgtctaa ggcacaggca accggcttct acggttccct gttgccaagc ccggacgtac
     1261 atggttataa gtccagcgaa atgctttaca actgggtagc aaaaggtgtt gaaccgacca
     1321 aattcaccga agttaccgac gtggtgctga tcacgcgtga caactttaaa gaagaactgg
     1381 agaaaaaagg tttaggcggt aagtaatttg ccggaaaaat tcccctttgc atgatgcaga
     1441 gggggtgtga acgaccagtg attcacggag acgttatgca acagtctacc ccgtatctct
     1501 catttcgcgg catcggtaaa acgtttcccg gcgttaaggc gctgacggat attagttttg
     1561 actgctatgc cggtcaggtt catgcgttga tgggtgaaaa tggcgcagga aaatcaactc
     1621 tcttaaaaat cctcagcggc aactatgcgc caaccacggg ttctgtagtg attaatgggc
     1681 aggaaatgtc cttttccgac acgaccgcag cacttaacgc gggtgtggcg attatttacc
     1741 aggaactgca tctcgtgccg gaaatgaccg tcgcggaaaa catctatctc ggccagctgc
     1801 cgcataaagg cggcattgtg aatcgctcat tgctgaatta tgaggcgggt ttacaactta
     1861 aacatcttgg tatggatatt gacccggaca cgccgctgaa atatctctcc attggtcagt
     1921 ggcagatggt tgaaatcgcc aaggcgctgg cgcgtaacgc taaaattatc gcctttgatg
     1981 agccaaccag ctccctttct gcccgcgaaa tcgacaatct tttccgcgtt attcgtgaac
     2041 tgcgaaaaga ggggcgggtg atcttatacg tttctcaccg tatggaagaa atatttgccc
     2101 tcagcgatgc catcactgtc tttaaagatg gacgttatgt caaaaccttt accgatatgc
     2161 agcaggttga ccacgacgcg ctggtgcagg cgatggtcgg gcgcgacatt ggcgatatct
     2221 acggctggca accgcgtagt tatggcgagg agcgcctgcg tcttgatgct gtgaaagcac
     2281 caggcgtgcg tacgccaata agtctggcgg ttcgcagtgg tgaaattgtt gggctgtttg
     2341 gtctggtagg ggcggggcgt agcgaattaa tgaaaggcat gtttggcggg acgcaaatca
     2401 ccgccggtca ggtttatatc gaccaacagc cgatcgatat tcgtaaaccg agccacgcca
     2461 ttgccgcagg catgatgctc tgcccggaag atcgcaaagc ggaaggcatt attcctgtgc
     2521 actccgttcg cgacaatatc aacatcagtg ccagacgtaa acatgtgctc ggcggttgtg
     2581 taatcaacaa cggttgggaa gaaaacaatg ccgatcacca cattcgttcg ctcaacatca
     2641 aaacgccggg cgctgagcaa ctgattatga atctctcagg tggaaatcag caaaaagcca
     2701 ttctgggccg ctggttatcg gaagagatga aggtcatttt gctggatgaa cctacgcgcg
     2761 gcattgatgt tggtgctaag cacgaaatat ataacgtgat ttatgcgctg gcggcgcagg
     2821 gtgtggcggt gctgtttgcc tccagcgact tacctgaagt cctcggcgtt gccgaccgga
     2881 ttgtggtgat gcgcgaaggt gaaatcgccg gtgaattgtt acacgagcag gcagatgagc
     2941 gtcaggcact gagccttgcg atgcctaaag tcagccaggc agttgcctga gtaaggagag
     3001 tatgatgtct tctgtttcta catcgggttc tggcgcacct aagtcgtcat tcagcttcgg
     3061 gcgtatctgg gatcagtacg gcatgctggt ggtgtttgcg gtgcttttta tcgcctgtgc
     3121 catttttgtc ccgaactttg ccacctttat taatatgaaa gggttgggcc tggcaatttc
     3181 catgtcgggg atggtggcgt gtggcatgtt gttctgcctt gcttccggtg actttgacct
     3241 ttctgtcgcc tccgtaattg cctgtgcggg tgtcaccacg gcggtggtta tcaatctgac
     3301 tgaaagcctg tggattggcg tggcagcggg gttgctgctt ggcattctct gtggcctggt
     3361 caatggcttt gttatcgcca aactgaaaat aaatgctctg atcacgacac tggcaacgat
     3421 gcagattgtt cgaggtctgg cgtacatcat ttccgacggt aaagcggtcg gtatcgaaga
     3481 tgaaagcttc tttgcccttg gttacgccaa ctggttcggt ctgcctgcgc caatctggct
     3541 caccgtcgcg tgtctgatta tctttggttt gctgctgaat aaaactacct ttggtcgtaa
     3601 taccctggcg attggcggga acgaagaggc tgcgcgtctg gcgggtgtac cggttgctcg
     3661 caccaaaatt attatctttg ttctctctgg cctggtatct gcgatagccg gaattattct
     3721 ggcttcacgt atgaccagtg ggcagccaat gacgtcgatt ggttatgagc ttattgttat
     3781 ctccgcctgc gttttaggtg gcgtttctct gaaaggtggc atcggaaaaa tctcatatgt
     3841 ggtggcgggt atcttaattt taggcaccgt ggaaaacgcc atgaacctgc ttaatatttc
     3901 tcctttcgcg cagtacgtgg ttcgcggctt aatcctgctg gcagcggtga tcttcgaccg
     3961 ttacaagcaa aaagcgaaac gcactgtctg atgctttttt ccacaacaat ttaacgtttt
     4021 ttcccaccac agccagccgc cacaacggtt ggctgttctt cgttgcaaat ggcgaccccc
     4081 gtcacactgt ctatacttac atgtctgtaa agcgcgttct gcgcaacaca ataagaaaag
     4141 agaaggagga gaaccgggtg acagaaccgt taaccgaaac ccctgaacta tccgcgaaat
     4201 atgcctggtt ttttgatctc gatggaacgc tggcggaaat caaaccgcat cccgatcagg
     4261 tcgtcgtacc tgacaatatt ctgcaaggac ttcagctact ggcaaccgca agtgatggtg
     4321 cattggcatt gatatcaggg cgctcaatgg tggagctgga cgcactggca aaaccttatc
     4381 gcttcccgtt agcgggcgtg catggggcgg agcgccgtga catcaatggt aaaacgcata
     4441 tcgttcatct gccggatgcg attgcacgtg atattaacgt gcaactgcat acggtcatcg
     4501 cccagtatcc cggtgcggag ctggagacga aagggatggc ttttgcgctg cattatcgtc
     4561 aggctccgca gcatgaagac gcattaatga cattagcgca acgtattact cagatctggc
     4621 cacaaatggc gttacagcag ggaaagtgtg ttgtcgagat caaaccgaga ggtaccagta
     4681 aaggtgaggc gattgctgcc tttatgcagg aagctccctt tatcgggcga acgcccgtat
     4741 ttctgggcga tgatttaacc gatgaatctg gcttcgcagt cgttaaccga ctgggcggaa
     4801 tgtcagtaaa aattggcaca ggcgcaactc aggcatcatg gcgactggcg ggcgtaccgg
     4861 atgtctggag ctggcttgaa atgataacca ccgcattaca acaaaaaaga gaaaataaca
     4921 ggagtgatga ctatgagtcg tttagtcgta gtatctaacc ggattgcacc accagatgag
     4981 cacgccgcca gtgccggtgg ccttgccgtt ggcatactgg gggcactgaa agccgcaggc
     5041 ggactgtggt ttggctggag tggtgaaaca gggaatgagg atcagccgct aaaaaaggtg
     5101 aaaaagggta acattacgtg ggcctctttt aacctcagcg aacaggacct tgacgaatac
     5161 tacaaccaat tctccaatgc cgttctctgg cctgcttttc attatcgcct cgatctggtg
     5221 caatttcagc gtcctgcctg ggacggctat ctacgcgtaa atgcgttgct ggcagataaa
     5281 ttactgccgc tgttgcaaga cgatgacatt atctggatcc acgattatca cctgttgcca
     5341 tttgcgcatg aattacgcaa acggggagtg aataatcgca ttggtttctt tctgcatatt
     5401 cctttcccga caccggaaat cttcaacgcg ctgccgacat atgacacctt gcttgaacag
     5461 ctttgtgatt atgatttgct gggtttccag acagaaaacg atcgtctggc gttcctggat
     5521 tgcctttcta acctgacccg cgtcacgaca cgtagcgcaa aaagccatac agcctggggc
     5581 aaagcgtttc gaacagaagt ttacccgatt ggcattgagc caaaagaaat agccaaacag
     5641 gctgccgggc cactgccgcc aaaactggcg caacttaaag cggaactgaa aaacgtacaa
     5701 aatatctttt ctgtcgaacg gctggattat tccaaaggtt tgccagagcg ttttctcgcc
     5761 tatgaagcgt tgctggaaaa atatccgcag catcatggta aaattcgtta tacccagatt
     5821 gcaccaacgt cgcgtggtga tgtgcaagcc tatcaggata ttcgtcatca gctcgaaaat
     5881 gaagccggac gaattaatgg taaatacggg caattaggct ggacgccgct ttattatttg
     5941 aatcagcatt ttgaccgtaa attactgatg aaaatattcc gctactctga cgtgggctta
     6001 gtgacgccac tgcgtgacgg gatgaacctg gtagcaaaag agtatgttgc cgctcaggac
     6061 ccagccaacc cgggcgttct tgttctttcg caatttgcgg gagcggcaaa cgagttaacg
     6121 tcggcgttaa ttgttaatcc ctacgatcgt gacgaagttg cagctgcgct ggatcgtgca
     6181 ttgactatgt cgctggcgga acgtatttcc cgtcatgcag aaatgctgga cgttatcgtg
     6241 aaaaacgata ttaaccactg gcaggagtgc ttcattagcg acctaaagca gatagttccg
     6301 cgaagcgcgg aaagccagca gcgcgataaa gttgctacct ttcctaagct tgcgtaggag
     6361 cggttaatct cccgtaagtg gaactaacag cacatcgacc tcacttgagg caatcactct
     6421 tttcgccgag caggatgctc gcgaaaagaa actgtgattg tgattaccgc aaatcaccaa
     6481 atcgaaatga tatttgcgac aaacttccag aatatgttcg cttaactcac cgtaggcaat
     6541 aaacgtcttg tcaacgggat accccgcatc ctgaattaac ttatcaagaa agctttgcgt
     6601 ttcttcctgc atcacgctgc gtaaatcttc aagcatcggc gcagctaatt gattgtacat
     6661 ttccgggtcg gaagcgagag taattaaact gatgtgtccc tttactggcc tggcgataga
     6721 taccgctttt gccagcagtt gctgactttc cggtgtaacc gcaacagcga caagaatatt
     6781 gctatagctc ataacctgtt ccttattctg tgaacttcag gtgacattaa agctttactc
     6841 ctaatggaga aacgacgcaa tcccaactca gtcaaacagg taaatattag tctgattatt
     6901 agtaaagaca attaatcaat cact
//
LOCUS       DAZKIP010000189         6922 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZKIP010000189 DAZKIP010000000
VERSION     DAZKIP010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.191"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..582)
                     /gene="yeeO"
                     /locus_tag="ACHOUK_004453"
     CDS             complement(<1..582)
                     /gene="yeeO"
                     /locus_tag="ACHOUK_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFP9899765.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     FAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPL"
     gene            743..818
                     /locus_tag="ACHOUK_004454"
     tRNA            743..818
                     /locus_tag="ACHOUK_004454"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:776..778,aa:Asn,seq:gtt)
     gene            complement(856..1806)
                     /gene="cbl"
                     /locus_tag="ACHOUK_004455"
     CDS             complement(856..1806)
                     /gene="cbl"
                     /locus_tag="ACHOUK_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFP9899766.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLHNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPHDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIILSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(1908..2825)
                     /gene="nac"
                     /locus_tag="ACHOUK_004456"
     CDS             complement(1908..2825)
                     /gene="nac"
                     /locus_tag="ACHOUK_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFP9899767.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            3151..3226
                     /locus_tag="ACHOUK_004457"
     tRNA            3151..3226
                     /locus_tag="ACHOUK_004457"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3184..3186,aa:Asn,seq:gtt)
     gene            complement(3282..4217)
                     /gene="ldtA"
                     /locus_tag="ACHOUK_004458"
     CDS             complement(3282..4217)
                     /gene="ldtA"
                     /locus_tag="ACHOUK_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFP9899768.1"
                     /translation="MMRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPVTVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(4279..5358)
                     /gene="cobT"
                     /locus_tag="ACHOUK_004459"
     CDS             complement(4279..5358)
                     /gene="cobT"
                     /locus_tag="ACHOUK_004459"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFP9899769.1"
                     /translation="MQTLANLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALGV
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVNVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPFLNMDMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASKIVLPGNTTSDLNS"
     gene            complement(5370..6113)
                     /gene="cobS"
                     /locus_tag="ACHOUK_004460"
     CDS             complement(5370..6113)
                     /gene="cobS"
                     /locus_tag="ACHOUK_004460"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFP9899770.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLVLMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(6110..6655)
                     /gene="cobU"
                     /locus_tag="ACHOUK_004461"
     CDS             complement(6110..6655)
                     /gene="cobU"
                     /locus_tag="ACHOUK_004461"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFP9899771.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEVVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(6807..>6922)
                     /locus_tag="ACHOUK_004462"
     CDS             complement(6807..>6922)
                     /locus_tag="ACHOUK_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HFP9899772.1"
                     /translation="VPAVALIHYLNEMCQSTETAVNDAFCRTLTRESLAIP"
BASE COUNT         1866 a         1873 c         1523 g         1660 t
ORIGIN      
        1 tagcggtatt ttcgtattcc ctgcaccacg aagtgccccg ctaccaatta gagtgatggc
       61 agctgctgga taactgagta ccgtcagctc cagataagtc aacgccagtg ctttaacttc
      121 tgtcgtggca tcacccgcga cgaaatcaat aatttgctcg ccaaaatgat gaataagcgt
      181 tgccaacagt acggcaaaca acgtcatgat caccaatgac tgccgcgtcg ccaccctcgc
      241 tcgtcgtcga tcccgcttac cgagactaaa tgccacaacg acagtagtac caagatcgat
      301 agcagcaaaa aaagccataa tgaccatatt gaagctgtcc gccaatccca cgccggccat
      361 cgcatctttt cccagccagc tgaccagaaa agtgctcaga acccccatca acaggacaca
      421 ggcattctcc atgaagatag gaacagcaaa cggggttatc tcgcgccaga acaacacttt
      481 gtagctcttg cgtttagcgt gccagcgagt gccgtgaaca acctggcgta aagcagagga
      541 gatattcaaa gccgacctta attgcagaaa gtgaaaccac atttcaaata atgagggaga
      601 atcagcaaag ctgcaaagat tttcgccatc aaattgtctg caaatgcaac aaactgttga
      661 tagaaacggc aaacagttgg gaaaattaaa aatcgggttt gacaaaagat ttttcgccgt
      721 taagatgtgc ctcaacagcg attcctctgt agttcagtcg gtagaacggc ggactgttaa
      781 tccgtatgtc actggttcga gtccagtcag aggagccaaa tttctgaaaa gcccgctttt
      841 atagcgggct ttttgctata tctgataatc aatttcctct tcactgtttt ccatcacctg
      901 ccgcttgata tcctcaactg aaagtcctgc attgcaaagt tccagaaagc gccagacata
      961 gttacgctga agttgtcctc gcttcagtcc caaccagaca gtattagcat cgaaaagatg
     1021 ccgcgtatcc aggcggatta aattcttttc ctcttgttca ccgctggatt gttcggcaac
     1081 taatccgatc ccaagcccaa gagcaacata ggttttaatg acatcagaat cctgcgcact
     1141 taatataata tctgccagca aacctttgcg ggcaaatgcg tcatcaatac gtgagcgccc
     1201 cgtaatcccc tgtcggtaag tgattaacgg ccacttcgct attgattcca gcgtcaatgg
     1261 tgtaatttgc gtcaagggat gatcgtgtgg aacaagcaaa ctatggtgcc aacgaaacca
     1321 cgggaaggcg acgagctgcg ggtcattact caaacgctcg ctggcgatac caatatcagc
     1381 ttcaccatta tgcaacaacg tcgcaatttc ctgtggcgtc ccctggatta gctcgagccg
     1441 aacctccggg aaaagttcgc gaaaagcttt aatgacctct ggcaagctat aacgtgcctg
     1501 agtatgcgtc gttgcaatag tgagaacgcc agacgtatcg ttggtaaaca ggtctgcaag
     1561 ccgacgaaca ttactggctt cattgagaat acgttctgca atgaccagta atgctttgcc
     1621 cggttcagtc atgcccagca gtcgcttacc tcgtcgaaca aatatttcga tgccaagttc
     1681 atcctccagt tcccgaatat gacggctgac gcctgactgt gaggtaaaaa gcatattcgc
     1741 aacctctgtc aggttgtaat cctgacgtgc agcttcgcgg attatcttta gttgttggaa
     1801 attcacggta aactccgggc agttcagata tcccgttatt gttaaagtct aacgcccggc
     1861 ataacaaata ataaaaaccc gcatcttatt ccatcccgat ataacgctta gctcaccaat
     1921 tgccactgcc ttttttccat cactggagaa ctaatcactg acattaacaa ctctttcact
     1981 gcctgtgcct gtggcgataa gttcgctctg gcgggtaaat ttaatgacaa agagagactc
     2041 atggaaggcg tggtaatgcg tgacatccac ccatttactg cgccacataa cgaacgcgcg
     2101 gccgattcgg gtaatactgc aacgcccatg ccgctggcaa tcgctgcggt aagcgtagca
     2161 atagactcaa tttcaccaat gacttttgcc gtgagccgcc gtagggaaaa cgcctcatca
     2221 acacgaagtc taacagcgct gtaatcactg gggagaaaaa ggttcatttg tgcaatagca
     2281 ttaacatcaa cgctttgccc cgggcaatct tgagttccta ccaggaaaag atcttctttc
     2341 agcaaagcct gactggatac accagccaca ggggaatgct cataaatcac cgccatatcg
     2401 agttggtgat ttatcagttt ttcgttaagc actgcaccgc tattttcatg aagatagata
     2461 acgacctccg ggaattcagc gcgaaccgcc tgtaataagg gcatggtgat ggatgacgca
     2521 gcggttcctg gcgcaaagcc aatcgagact tgccccgata atgcctgacc aacgttatgc
     2581 accgccagtt gggcctgctc acactgacgt aaaatggccc gcgcatgggt atagagaatt
     2641 tttccggcgt ctgttggcgt aacgccccgt tttgtgcgaa tcaaaagttg ttgatttaac
     2701 tcaccttcga gtgtggcaac ctgctggctg agcgctggtt gtgcgatatg caatacttca
     2761 gcagcctgag tcaggctacc aatatctaca atttttacga agtatttcag gcgtctgaag
     2821 ttcatgttgc ctccggtttt tgagaatcgg cccaagtgcc gccattactt acaaccagat
     2881 tgcaagatgc ttgccagttt tattttggcg ttgatgtaca agctaaccaa ctgtcaaata
     2941 agagattatg atagattcgt cattggctct tttcaccagc agtcgcgttc ccctgcccta
     3001 taaaaggagg gtatgcacca cgatggttca ttaccaataa gattgaaatc tcatcacttt
     3061 gttgaaattg acagcaaaca aacaaaaaaa tgcatttcac cctttgacat caccatgcac
     3121 tgccattaat atgcgccccg ttcacacgat tcctctgtag ttcagtcggt agaacggcgg
     3181 actgttaatc cgtatgtcac tggttcgagt ccagtcagag gagccaaatt caaaaaagcc
     3241 tgctttctag caggcttttt gctttctaat taccaacgct cttaaaacat ctgtcttgaa
     3301 ccagaactaa tttgcacagg cattcccgat cgacgttgca acgcagcatt tgcgcgattc
     3361 acatcaactt cttgcccgtt gataaacgcc cgcaaagatg gggttaccgg caatggcact
     3421 tttcggtcag actcatattc agcacgattg cgcgacagcg gctcatgaac ttccagccac
     3481 tttgaaccat ccggttccgt tgtgtatttc actggctgat caataatttg cacacgagta
     3541 cctacaggaa cattatcaaa cagatatttg atatcgtcat tgcgcagacg aatacagccc
     3601 tgacttaccc ggagcccaat accaaaattg gcattggtac cgtggatggc atatagcctg
     3661 ccaatataaa tcgcgtacag ccccatggga ttatcgggcc ccgcaggaac aaatgcgggc
     3721 aaactctccc ctcgtttcgc atattcgcgc cgggtgttcg gcgttggcgt ccaggttggt
     3781 gcttcttgtt tacgttcaac ggtagtcacc cagttacgcg gggtttctcg cccagcctgg
     3841 ccgataccaa taggaaagac ttccacagta ttactgtctg gtgggtagta ataaagacgc
     3901 atctcagcga cgttaaccac aatcccttta cgaacagtga cgggcaaaat cagttgctgc
     3961 ggaatggtga gttgcgaacc tgacttcggt aagaagacat cagcgcccgg attcgcttcc
     4021 agcatgttac ttaacccttg cccgtattgt gcggcaaaag tctccagcgg ctgggtattg
     4081 tgatcaggaa cagttacagt aagcgactgc cccactaaac ggctaccctc tggaggtaat
     4141 ggataagtta ccgccaggct agtatggctg gcaaaaagca gagcaaatga gcaaagaata
     4201 tttacacgac gcatcatgtc cctttcctaa gtcgcgaaag ctatcggtta agtatagctt
     4261 ttatcagact tttcgttttt aactgttcaa atcagaagtc gtattccccg gtagaacaat
     4321 tttactggca gcaagttcgc ccatgttgtt gtatatcgca caagcagctt cgatgatggg
     4381 catcgccaga gctgcgccac tcccctcacc taaacgcata tccatattga gaaaaggctc
     4441 cagccccaaa tgcgacagcg ctatacgcgc ccctttttct gccgacaggt gagaaggaat
     4501 gagatacggt ttgattgcag gagacatctg gcaggctgcg agcgcagcag cataagaaag
     4561 aaatccatcc agcaacacgg gtaaaccgca ggaagcagcg cctaacatca cgccagccat
     4621 tccgaccaaa tcaaatccac ccacttttgc cagaacatta acgccatcct gcggatttgg
     4681 ctgattcaac gtaatcgccc ggcgcacaac atcaatttta ttagccagtt tatctgtcgg
     4741 caggtttgcg ccaatcccaa ccacttcttc aggatcccgg ccagtgattg tgctgactat
     4801 tgctgctgcc ggtgtcgtgt ttgccatccc cagttcacct acaccaaaca gcgtgacacc
     4861 gttttttgcc agctcccgcg tataacatat gacgtccaaa agtaactttt cagcctgacg
     4921 gcgactcatt gccggagctg aagcaatatt gccgctacct cgtgcgacac gcatgttgat
     4981 aagcccgggg ataggctcag cagtatcaat accaacatca attacgtgga cgttagcgcc
     5041 cgcttgtgct gccagcacac acacgccggt tgttccgcgg gtcatatttt cagcctgaat
     5101 ggctgtcact tcttttgggg aaatagcgac cccttcctcc cagacgccgt gatcggcaca
     5161 cataaccagt accgcttttt tgcccacatg cggtatgcca ttcaaccccg gcattcccgc
     5221 cagttgtacg ccaagcgcct ccagccttcc caggctacca acaggtttga gtaacccgtc
     5281 aatatgccgt tgtgcacgcg acatagcggc aggatcgatg gcagggatcg tattcagtaa
     5341 attggcaagt gtttgcatgt cacgtctctt tatagcagtg ccagcaggaa gaccagttca
     5401 ccaagctcta tggctgctcc cagcgtatcg ccggtttgcc cgcccagcgt acgtttgaga
     5461 agctgaccga ggatgaaaat cgccaccatc gtcaccacca tagcagccac accatgcata
     5521 cccggcaata atacggcagc aaaaatagcg gctaagccga gtgtgacgca ggtttgtcgc
     5581 ccatcaattt tgccaatgaa cacattgcca agcccttctt cccgggcata acgatgacga
     5641 tacatcaata atgcggcagt cccgcggctg accgcacatg ctgccgccag cgatgcaagg
     5701 atcggttcgc cacgcagagc caactcgctt aacaccaaaa tctttgccag tacaacaaaa
     5761 atcaatgcca gaccgccgtg ggtacctaaa cggctatcac gcatgatttc caacatgcga
     5821 tcgcggctac gtgcagaaaa tacgccatca caggtatcag cgaggccgtc cagatggaat
     5881 cccccggtca tcagcaccag cacaagtacg ctaaacagtg ccgccagtgg tgcgccacac
     5941 catgcctgca gcaccatgaa gaccagcccg ctaatcgcgc caagcaataa tccaatcaaa
     6001 ggaaaagtaa taataccgcg agaataatgc tcgaaatcca gtccctggga ccagcgacgc
     6061 ggtacaggca gacgcgtaat aaatgagagc atcgcccaaa ataatttact catttgattt
     6121 ttactccaat acccgaaacc accagccata cttcatttgc cgcagcggcc aactgctgat
     6181 ttacccgccc ggcaatatca cgaaaatgtc gtgccagacg actctccggc acaatcccca
     6241 ttcccacttc gttagtcact aatacaacct ttgcggggca acgttggcag gcagcaatca
     6301 acgactgaat ctcagcatta atcgcctgtt ccatcgcctg ataatcccat tcatcagggt
     6361 ctttatcgcc gccataatca aacaacagat tagtcaccat tgtggtaacg cattcaagca
     6421 acacaacctc attcgggtta atgtctgcat gaattaattc atcaagatgt tgccagcgct
     6481 ccactgtgcg ccagtgctcc gggcggcctt gccgatgatg ttctatccgt gcagccatct
     6541 catcatcaag gatttgcgag gtagcgatat acagaacctg tgaagagtcc ccaataagcg
     6601 cctctgcgtg gcgactcttc ccgctccgtg cgccccccgt caccaaaatc atcataccga
     6661 ctcctgattc aaatgtatga tggtattcat tgaacagaac aatggatccc ccaccgttgc
     6721 tgaccgcaaa acatttacgc gattaatact gcgcgtaata taattaaaaa tccagcattc
     6781 tcaatccatt acgaaataag atggcattac ggaatagcca gactttctcg tgttaacgtc
     6841 cgacaaaatg cgtcattgac agcggtttct gtggattgac acatttcatt cagataatga
     6901 attaatgcta ctgccggaac aa
//
LOCUS       DAZKIP010000190         6713 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZKIP010000190 DAZKIP010000000
VERSION     DAZKIP010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6713
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.192"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(258..2540)
                     /locus_tag="ACHOUK_004463"
     CDS             complement(258..2540)
                     /locus_tag="ACHOUK_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFP9899773.1"
                     /translation="MAVKASGRFVPPSAFAAGTGKAFTGAYAWNAPREAVGRERPLTR
                     DEMRQVQGVLSTINRLPYFLRSLFTSRYDHIRRNKSPVHGFYFLTSTFQRRLWPRIER
                     VNQRHEMNTDASLLFLAERDHYARLPGMNDKELKKFAARISSQLFMMYGELSDAWVDA
                     HGEKESLFTDEAQAHLYGHVAGAARAFNISPLYWKIYRKGQMTTRQAYSAIARLFNDE
                     WWTHQLKGQRMRWHEALLIAVGEVNKDRSPYASKHAIRDVRARRQANLEFLKSCDLEN
                     RETGERIDLISKVMGSISNPEIRRMELMNTIAGIERYAAAEGDVGMFITLTAPSKYHP
                     TRQVRKGESKTVQLNHGWNDEAFNPKDAQRYLCRIWSLMRTAFKDNDLQVYGLRVVEP
                     HHDGTPHWHMMLFCNPRQRNQIIEIMRRYALKEDGDERGAARNRFQAKHLNRGGAAGY
                     IAKYISKNIDGYALDGQLDNDTGKPLKDTAAAVTAWASTWRIPQFKTVGLPTMGAYRE
                     LRKLPRGVSIADEFDERVEAARAAADSGDFALYISAQGGANVPRDCQTVRVARSPSDD
                     VNEYEEEVERVVGIYAPHLGARHIHITRTTDWRIVPKVPVVEPLTLKSGIAAPRSPVN
                     NCGKLTGGDTSLPAPTPTEHAAAVLNLIDEGILSWTEPGIMKVLRDLLSNELKCSNRS
                     QQSFTPFNGRSYFPAPSARLTRQERKTIPKIKVLLAQSDIQASYWELEALARGAVLDF
                     GHKRFKFDNDIDFFDRQREW"
     gene            complement(2530..2805)
                     /locus_tag="ACHOUK_004464"
     CDS             complement(2530..2805)
                     /locus_tag="ACHOUK_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFP9899774.1"
                     /translation="MSIRIEIGERYVVTSDSFQFILHEKKRAESGKNAGQEWLAVVGY
                     YPKLSQLVSGLMHHDILTGSAKSFADLNAQVEQLSKRCSEAFGSYGR"
     gene            complement(2802..3026)
                     /locus_tag="ACHOUK_004465"
     CDS             complement(2802..3026)
                     /locus_tag="ACHOUK_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HFP9899775.1"
                     /translation="MPDNVDFIQEQQAELLERQINAARVKHCGASALVCEECDAPIPA
                     ARRAAYPSATRCVSCQSVFEAKNKRYRRTA"
     gene            complement(3029..3328)
                     /locus_tag="ACHOUK_004466"
     CDS             complement(3029..3328)
                     /locus_tag="ACHOUK_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFP9899776.1"
                     /translation="MSCSHSVVLLNNALKIAVMKNGDLSLIQLCLDKEKRDITESVIA
                     IYQNELNLLSDVVNLLVKRAVFHKQISSVDELTKLTTELASYCADVSRKLNDKRS"
     gene            complement(3328..3552)
                     /locus_tag="ACHOUK_004467"
     CDS             complement(3328..3552)
                     /locus_tag="ACHOUK_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HFP9899777.1"
                     /translation="MHTVSENKCGKYALLLQQARAEAQADAATRFSSHLDAMIRHITK
                     AELSRVEIVELLSQESEKFHNIGLSRGEVL"
     gene            complement(3616..4116)
                     /locus_tag="ACHOUK_004468"
     CDS             complement(3616..4116)
                     /locus_tag="ACHOUK_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein B"
                     /protein_id="HFP9899778.1"
                     /translation="MTVMTLNLVEKQPAAMRRIIGKHLAVPRWQDTCDYYNQMMERER
                     LTVCFHAQLKQRHATMRFEEMNDVERERLVCAIDELRGAFSKRRQVGASEYAYISFLT
                     VSQRRTLFMHAGLTEKEFNQPYWRINEESCYWRDALFRALRELFSLFEYAPTILTSVK
                     PEQYLH"
     gene            complement(4113..4283)
                     /locus_tag="ACHOUK_004469"
     CDS             complement(4113..4283)
                     /locus_tag="ACHOUK_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899779.1"
                     /translation="MNEPRCIAQLLRNESPRAIDFTITHGKGRKGIIIRTKKQSPLKK
                     ALTFLKSRRVWK"
     gene            complement(4294..4569)
                     /locus_tag="ACHOUK_004470"
     CDS             complement(4294..4569)
                     /locus_tag="ACHOUK_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory phage cox family protein"
                     /protein_id="HFP9899780.1"
                     /translation="MNRQLVSVTDAVPYQEFAKLIGKTPRAVRGMIEKGKLPVIEITD
                     PQSVSGRAGEYWVYLPAWNNGLKLAYESRPKEIRDGWLMWLGLGEPR"
     gene            4691..4990
                     /locus_tag="ACHOUK_004471"
     CDS             4691..4990
                     /locus_tag="ACHOUK_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9899781.1"
                     /translation="MSIDVSEKLKLIRESERLNRKEFSELTGVAYSSLSSYESRSKNA
                     GVEAIMKVLQHPRFTKYTLWFMTDQVAPEAGQIAPALAHFGQNETTSPHSGQKTG"
     gene            5106..6119
                     /locus_tag="ACHOUK_004472"
     CDS             5106..6119
                     /locus_tag="ACHOUK_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFP9899782.1"
                     /translation="MAIKKLDDGRYEVDIRPTGRNGKRIRRKFDKKSEAVAFEKYTLY
                     NHHNKEWLSKPTDKRRLSELTQIWWDLKGKHEEHGKSNLGKIEIFTKITNDPCAFQIT
                     KSLISQYCATRRSQGIKPSSINRDLTCISGMFTALIEAELFFGEHPIRGTKRLKEEKP
                     ETGYLTQEEIALLLAALDGDNKKIAILCLSTGARWGEAARLKAENIIHNRVTFVKTKT
                     NKPRTVPISEAVAKMIADNKRGFLFPDADYPRFRRTMKAIKPDLPMGQATHALRHSFA
                     THFMINGGSIITLQRILGHTRIEQTMVYAHFAPEYLQDAISLNPLRGGTEAESVHTVS
                     TVE"
     gene            complement(6384..6701)
                     /gene="yegR"
                     /locus_tag="ACHOUK_004473"
     CDS             complement(6384..6701)
                     /gene="yegR"
                     /locus_tag="ACHOUK_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFP9899783.1"
                     /translation="MKKIAAISLISVFLMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
BASE COUNT         1825 a         1688 c         1447 g         1753 t
ORIGIN      
        1 gaaacatgca atatcaaata cattgccaat gtcttcatct tctgctaata gttttggaaa
       61 tgaagtttct ttattaaagt tccatgtttc caaaccacca tttaatccat gtacaaatag
      121 tatcaggcta tcttttttct cgtggcttat ccattcaacc atgctcatat gcttttctgc
      181 cgtgtataaa aattaaacta taacaaacaa ttcctcaaca ataacatatt gttaccccgt
      241 ttgaccgttt ttttcaacta ccactcacgc tgtctgtcaa aaaagtcgat atcgttatca
      301 aatttaaaac gcttatgccc aaaatccaaa acagctccgc gagcgagagc ttcaagctcc
      361 caataactag cctgaatgtc actttgtgca agaagaacct taattttcgg gatagtcttt
      421 ctctcctgcc ttgtcaaccg ggcggatggg gcaggaaagt agcttcgacc attgaaaggg
      481 gtaaagctct gctgtgaacg attactgcat ttcagttcat tactcaataa gtctctaagt
      541 accttcataa tgcctggctc ggtccaactt agaatccctt catctattaa atttaacacc
      601 gctgcagcat gctcagtcgg tgtgggagcc ggtaacgaag tatcaccacc ggtgagtttt
      661 ccacagttat tgacaggact ccgaggcgcg gcgatgccgc tttttaaagt caaaggctca
      721 acgaccggaa ctttcggcac aatgcgccag tccgtcgttc tggtgatatg aatatgacgc
      781 gcgccgagat gcggcgcgta aatgccgacc actctctcga cttcttcctc gtactcgtta
      841 acgtcatccg acgggctacg ggcgaccctg acagtctgac aatcgcgcgg gacatttgcc
      901 ccaccctgcg cgctgatata caacgcaaaa tcaccactgt ctgcggcagc gcgagcagcc
      961 tcgacgcgtt cgtcaaactc atcagcaata ctgacgccgc gaggcaattt gcgtagttca
     1021 cggtaagccc ccattgtcgg cagtccaacc gttttaaatt gcggaatgcg ccacgttgac
     1081 gcccatgcgg taacagccgc ggcagtatct ttaagcggct taccggtatc gttatcgagc
     1141 tgaccatcca gtgcatagcc gtcgatgttt tttgaaatgt atttcgcgat atatcccgca
     1201 gcaccgcccc ggttaaggtg ttttgcctga aaacggtttc gcgcagctcc tctttcgtct
     1261 ccatcctctt tgagcgcgta gcgacgcatg atttcgataa tctggttacg ctggcgtgga
     1321 ttacaaaaaa gcatcatatg ccagtgcggc gttccgtcgt ggtgtggctc gacgacacgc
     1381 aaaccgtaga cctgtaaatc attatccttg aatgccgtgc gcatcaggct ccagatgcgg
     1441 cagagataac gctgcgcatc ctttggatta aatgcctcat cgttccagcc gtgattaagc
     1501 tgaacggttt tactttcgcc ttttctgacc tgacgtgtcg ggtgatactt tgacggcgcg
     1561 gtcagcgtga taaacatccc cacatcaccc tctgcggcgg cgtaacgctc aataccggca
     1621 atggtgttca tcagctccat ccggcgaatt tcaggattag aaatactgcc catcacctta
     1681 ctgataaggt cgatgcgctc gccggtttcc ctgttttcaa ggtcacacga tttaagaaat
     1741 tccagatttg cctggcggcg cgcacgcaca tcacgaatgg cgtgtttact ggcataagga
     1801 gaacggtctt tattgacctc cccgacagct atcagtaacg cttcatgcca gcgcatacgc
     1861 tggcctttaa gctgatgagt ccaccactca tcgttaaaca gacgggcaat ggcagaatat
     1921 gcctgcctcg tggtcatctg ccctttacgg tatattttcc agtagagagg ggaaatattg
     1981 aaagcacgtg cagcgccagc aacatgacca tagaggtgag cctgcgcctc atccgtaaac
     2041 agcgattctt tttcgccatg cgcatccacc caggcatcac tgagttcccc atacatcatg
     2101 aaaagctgcg atgagatacg ggcagcaaac tttttcagct ccttgtcatt cattcccggc
     2161 aggcgcgcat agtggtcacg ctctgccaga aacagtaacg acgcgtcggt gttcatttca
     2221 tggcgctgat tcacacgctc aatgcgcggc cataaacgac gctgaaaagt ggatgtgagg
     2281 aaataaaacc cgtgcaccgg gcttttattg cgccggatgt ggtcatagcg tgaagtaaac
     2341 agcgagcgca aaaagtaagg caggcggtta atcgtggata aaacaccttg cacctgacgc
     2401 atctcgtcac gtgtaagggg tctttcgcgc ccgacggcct cgcgtggcgc gttccatgca
     2461 taagcaccgg taaacgcctt accggtgcct gcagcaaatg ctgacggagg gacaaaacgc
     2521 ccggaggctt taacggccat atgagccaaa agcctctgag caacgcttgc tgagttgctc
     2581 aacctgcgcg tttaaatcag caaaagactt tgcgcttccg gtcagaatat cgtgatgcat
     2641 caggccggaa acgagctggc ttaatttcgg gtaataacca accaccgcca gccattcctg
     2701 accggcgttt ttaccgcttt ccgctctctt tttctcgtgg agaataaact gaaagctgtc
     2761 actggtaacg acataacgtt cgccaatttc aatacgaata ctcatgccgt tctccggtaa
     2821 cgtttgtttt ttgcttcaaa gactgactga caggaaacac aacgcgtggc tgacgggtaa
     2881 gccgcacgac gggcagcagg tattggcgcg tcacactctt cgcaaaccag cgcagaagca
     2941 ccgcaatgtt ttacccttgc cgcgttaatc tggcgctcca gtaattcagc ctgttgttcc
     3001 tgaataaaat ctacgttgtc cggcattatc agctcctttt atcgttaagt ttcctggata
     3061 catcagcgca ataactggca agttctgtcg ttaattttgt cagttcatcc actgaggaaa
     3121 tttgcttgtg gaatacagcg cgtttaacaa gtaaattgac cacatcagac aggaggttta
     3181 attcattctg ataaatcgcg ataacagatt cagttatgtc gcgtttttct ttatcaaggc
     3241 aaagttgaat aagagacaaa tcgccatttt tcataacggc gatttttaag gcgttattca
     3301 gtaatacaac tgaatgagaa caggacatca aagcacctcc ccgcgagaca atccgatatt
     3361 gtgaaatttt tccgactcct gactgagcag ctcgactatc tccacgcggg ataactccgc
     3421 ctttgtgata tggcgaatca tggcgtcaag atgagaagaa aagcgtgtcg ctgcgtcggc
     3481 ctgtgcttcg gctctggcct gttgcagcag taatgcgtat ttaccgcact tgttttcaga
     3541 aactgtatgc atgactttct ccaggcaaaa agaagccccg cacgattaag tgcgttaaaa
     3601 actctggtta attatttaat gcagatattg ctctggtttt accgacgtca gaattgtcgg
     3661 tgcatactca aacaggctga ataattcacg taatgcacgg aataaagcat cacgccagta
     3721 acatgattct tcattaattc gccagtatgg ctggttgaat tctttttcag tcaatccggc
     3781 atgcataaat aaagtacgac gctgactgac tgttaaaaaa ctaatatatg catactcact
     3841 tgcgccaacc tgacggcgtt ttgagaatgc cccacgcaat tcatcaattg cacaaaccag
     3901 ccgttcacgt tcgacgtcgt tcatttcttc aaaacgcatc gttgcgtgac gctgttttaa
     3961 ctgcgcatgg aagcaaaccg ttagccgttc gcgttccatc atctgattat aataatcaca
     4021 tgtatcctgc cagcgaggga cggcaagatg cttgccaatt atccggcgca tagctgctgg
     4081 ctgtttttca acgagattga gcgtcatcac tgtcatttcc agaccctccg gcttttcaga
     4141 aaggtcagag ccttttttaa cggactctgt tttttggtgc ggataatgat tcccttacgc
     4201 cccttaccgt gggtgatggt gaagtcaatc gccctggggc tttcgttacg caataactga
     4261 gcaatacaac gaggctcatt catacggttc tccttaacgt ggttcaccga gacctaacca
     4321 catcaaccag ccgtcacgaa tctctttagg acggctttca taagccagtt ttagtccgtt
     4381 attccatgcc ggaaggtata cccaatattc accagcacgc cccgatactg actgagggtc
     4441 agtaatctca ataactggta atttcccttt ctcaatcatg ccccttacag ctcttggagt
     4501 tttaccaatg agttttgcaa actcctgata aggcacggca tcagtcacgc ttacaagctg
     4561 tctattcatc tgctacgatt ctcccttagt gcttctaatg gctcctaatg gctaattatt
     4621 gcctaaaagg ataactccag aagcgcaaca tttcacacta tcagcaagaa attacgcaat
     4681 cggagtaatt atgtcaatag acgtttcgga gaagttgaag ctaatccgtg aatctgaaag
     4741 gttaaaccgt aaagaattca gtgaattaac tggtgtagcc tacagctcac tttcgagcta
     4801 tgagagccgg tcaaaaaacg ctggagttga agccataatg aaggtcttac aacatcctag
     4861 atttactaaa tatactttgt ggttcatgac tgatcaggta gctccagaag ccgggcaaat
     4921 tgcgcccgct ctcgcacact ttgggcaaaa cgaaacaacg tcgccccact ccggtcaaaa
     4981 gactggttaa caatttatcg tgaatatatt catcacaagt gcctactatt ggtggctaaa
     5041 tttcagccac cacgaaaaaa gcgattagta gtagcaaaaa aaagtaccac tcggagggtt
     5101 ttctgatggc aatcaaaaaa ctcgatgatg gtcgatatga agtggacatc cgccctactg
     5161 gacgtaacgg aaaacgcatc cgtaggaagt ttgataagaa aagcgaagct gtcgctttcg
     5221 aaaaatacac gttgtacaac caccacaata aagaatggct atcaaaacca acagacaaac
     5281 gacgtctgtc ggaactgaca cagatctggt gggatttaaa gggtaaacac gaagagcatg
     5341 ggaaatctaa tcttggaaaa attgaaatct tcacaaaaat aacgaatgac ccatgcgcat
     5401 ttcaaatcac gaaatccctt atcagccagt actgcgccac ccgaagaagt cagggtatta
     5461 aaccttcgag tatcaatcgt gatttaacat gtattagcgg catgtttaca gccctgattg
     5521 aagcggagtt attctttggt gagcatccta tcagagggac aaagaggctt aaggaggaaa
     5581 aaccagaaac aggctatctc acacaggaag aaatagcctt actgcttgca gcacttgacg
     5641 gcgacaataa aaagattgcg attctttgcc taagtacagg agcacgttgg ggagaagcag
     5701 ctcgtttgaa agcagaaaat atcatccata accgcgtcac gtttgttaaa acgaaaacaa
     5761 acaaaccacg caccgtcccg atctcagagg ctgttgccaa aatgatcgcg gataacaaac
     5821 gaggtttttt attccctgat gctgattacc ctcgcttcag acgaacaatg aaagcaataa
     5881 aaccggattt gccaatgggg caagccacac atgcactaag gcacagcttt gccactcatt
     5941 tcatgattaa tggaggaagt attatcacgc tacaacggat actaggtcac acgcggattg
     6001 agcaaactat ggtttacgct cattttgcgc cagagtacct tcaggacgcc atttctctta
     6061 atccgctaag aggtggtact gaggccgaga gtgtccacac agtgtccaca gtagagtaac
     6121 gtttaagggc tttcagtggt aatttatgcc gctcaaaccc gcattgtacc gttgaaagcc
     6181 cctactggtg acaccctaaa tctcccttac acgggcttat tttttatgca taagccctat
     6241 ccctgataac cgtcttccat tgaccacatc gatagaatct cccttcatag cacgatgcct
     6301 ttcacgtaac ggcatcgtgc tcgcacaggt tccggctaag cacaaccaga acgcgcatgt
     6361 ttgacgctta ccaaaaaata ttctcactct ccacatttga atgtcagacg agcgacgcca
     6421 tgtaatcctg caccttctgt cttcaggtca actatctgca tttttttgcc ctgagtaaca
     6481 cagaaatggg ctgcatcatt ttttactata ttttctgcac cagatattct gcccctggct
     6541 aaagaagctt cggcttcggt gtagtattgg ttatcgagtt tacgctgaat attactttta
     6601 tatgcaagac caaatttacc gatacttgtc tcatcattat gcacagcaca accagacata
     6661 agaaaaacac taattaatga tatagcagct atctttttca tctcaccttc ccc
//
LOCUS       DAZKIP010000191         6656 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZKIP010000191 DAZKIP010000000
VERSION     DAZKIP010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.193"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            112..354
                     /gene="yjbE"
                     /locus_tag="ACHOUK_004474"
     CDS             112..354
                     /gene="yjbE"
                     /locus_tag="ACHOUK_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFP9899784.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            468..1106
                     /locus_tag="ACHOUK_004475"
     CDS             468..1106
                     /locus_tag="ACHOUK_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFP9899785.1"
                     /translation="MKRPAFILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIIDGATWMRTMGWTEYQQVRYATARSVFKWDGTGTVKVGSDET
                     AVRVLDEEVSTDQARWHNRYWIDSGGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1103..1840
                     /locus_tag="ACHOUK_004476"
     CDS             1103..1840
                     /locus_tag="ACHOUK_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFP9899786.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERANPPLQGNYTLWVGPPPSTVTLFGLISRPGNQP
                     FTPGRDVASYLSGQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSEMPDALNADILQTLTQRIPQ"
     gene            1840..3936
                     /locus_tag="ACHOUK_004477"
     CDS             1840..3936
                     /locus_tag="ACHOUK_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFP9899787.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERANTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIVRANRIVMNDLPDGIKTIRVTENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3983..4261)
                     /locus_tag="ACHOUK_004478"
     CDS             complement(3983..4261)
                     /locus_tag="ACHOUK_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899788.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSALPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4262..4405
                     /locus_tag="ACHOUK_004479"
     CDS             4262..4405
                     /locus_tag="ACHOUK_004479"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899789.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            4531..4941
                     /gene="psiE"
                     /locus_tag="ACHOUK_004480"
     CDS             4531..4941
                     /gene="psiE"
                     /locus_tag="ACHOUK_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFP9899790.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQASKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVVTLWLCNSKRLKRE"
     gene            complement(4985..6460)
                     /gene="xylE"
                     /locus_tag="ACHOUK_004481"
     CDS             complement(4985..6460)
                     /gene="xylE"
                     /locus_tag="ACHOUK_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFP9899791.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAEGILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
BASE COUNT         1643 a         1686 c         1796 g         1531 t
ORIGIN      
        1 ccccatctct aattccccgc gccgtaattc gcatgcttta gttgtgtata ctcgatcccg
       61 cccgaaatgt ttttgggtaa atctccattc attcaatgaa gggaaattgt tatgaaaaaa
      121 gttctgtatg gcatttttgc catatctgcg cttgcggcga cttctgcgtg ggctgcacct
      181 gtacaggtgg gcgaagcggc agggtcggca gcaacgtcgg tttcggcggg gagttcctcc
      241 gcgaccagcg tcagcaccgt aagctcggcg gtgggtgtcg cgctcgcggc aaccggtggc
      301 ggtgatggtt ctaataccgg gaccaccaca accacgacca ccagtaccca gtaataaagt
      361 atgtatcccc aaaataattc gagtcattgc atctgtggct agaagtatga agggatttac
      421 cataaccaca ctccggtgtg gttattctgc ccctctggag aagagtcgtg aagcgacctg
      481 cattcattct tatctgcctg ctattacagg cctgttcagc cacgactaaa gagctgggca
      541 attcactgtg ggacagtctg ttcggcacgc caggcgtaca gctgacggac gatgatattc
      601 aaaatatgcc ctacgccagc cagtacatgc agcttaatgg tgggccgcag ttgtttgtgg
      661 tgctggcctt cgctgaagac ggacaacaaa aatgggtcac tcaggatcag gccactctcg
      721 tcacacaaca tggtcgtctg gtgaagactt tgcttggcgg cgacaacctg attgaagtga
      781 ataaccttgc cgccgacccg ctgattaaac ccgcacaaat cattgatggc gcaacctgga
      841 tgcgcacgat gggctggacc gagtaccagc aggtacgcta cgccaccgca cgctcagtct
      901 tcaaatggga tggcaccggt accgttaagg tcggcagcga tgaaaccgcg gttcgcgtgc
      961 tggacgaaga agtctccacc gaccaggcgc gctggcataa ccgctattgg atcgacagcg
     1021 gagggcaaat tcgccagtcg gaacagtatc tcggcgcgga ttatttcccg gtgaaaacca
     1081 ctctcatcaa ggcggcaaaa caatgattaa acaaactatt gtcgcgttgc ttttgagtgt
     1141 gggagcgtca tcggtcttcg cggcaggaac cgtcaaggtg ttcagcaatg gcagcagtga
     1201 ggccaaaacg ctaacgggcg cagagcattt aatcgatctg gtaggccaac cgcggctggc
     1261 aaacagctgg tggcctggtg cggtgattag cgaagagctg gcaacggcgg cagcattgcg
     1321 tcagcagcag gcgttgctga cacggctggc agaacagggc gcagattcca gcgccgacga
     1381 tgccgctgcg attaacgcct tacgccagca aattcaggcg ttgaaggtga cgggcaggca
     1441 aaaaatcaat cttgatcccg atatcgtccg cgttgctgaa cgcgctaacc cgccgttgca
     1501 gggcaactac acgctgtggg tcggaccacc accgtccacg gtcacgttgt tcgggcttat
     1561 cagccgtcct ggcaatcagc cattcactcc cggtcgcgac gtggcgagct atctctccgg
     1621 gcaaagtctg cttagcggtg cggatcgtag ctacgcgtgg gtggtttacc cggacggacg
     1681 cacacaaaaa gcgccggtgg cttactggaa caagcgtcac gtagagccga tgcccggcag
     1741 cattatttat gttggcctcg cggactccgt ctggagtgag atgcctgatg cccttaacgc
     1801 cgacattctt cagactctga cgcagcggat acctcaataa tgaaaaaaag acatctgctt
     1861 agcttactgg cgctgggcat tagcacagct tgctacggcg aaacatatcc tgcgcccatt
     1921 ggtccgtcgc agtcggattt cggtggcgta ggattattac aaacgcccac ggctcgcatg
     1981 gcgcgggaag gggagttgag tctgaactat cgcgataacg atcagtaccg ttattactca
     2041 gcttcagtgc aactcttccc gtggctggaa acaacgctgc gctacaccga cgtgcgcacc
     2101 cggcagtaca gcagcgtcga agcgttctct ggcgatcaaa cgtataaaga taaagccttc
     2161 gatctcaaac tgcgtttgtg ggaagagagt tactggctgc cgcaagtggc ggttggtgcg
     2221 cgggatatcg gcggtacggg gctgtttgat gcggaatatc ttgttgccag caaagcctgg
     2281 gggccgttcg attttacgct tggcctgggc tggggatatt tgggcacaag cggtaatgtg
     2341 aaaaatccgc tctgttcagc cagtgataaa tattgctatc gcgataacag ctacaaacag
     2401 gcgggatcta tcgacggtag ccagatgttc cacggtcctg cctcactgtt tggcggcgtg
     2461 gaataccaga cgccctggca accgctgcgc ctgaaactgg agtatgaagg caataattat
     2521 cagcaggatt ttgccggtaa gctggagcaa aaaagtaagt ttaacgtcgg ggcgatttat
     2581 cgcgttaccg attgggccga cgttaacctt agctatgaac gtgccaacac ctttatgttt
     2641 ggcgtcacgc tgcgcaccaa ctttaacgat ctgcgcccgt cttacaacga taacgcccgc
     2701 ccgcaatatc aaccgcagcc gcaggatgcc attttgcagc attcggtggt ggcgaatcag
     2761 ttaacgctgt tgaaatacaa tgccggactt gccgatccgc agatccaggc gaaaggcgat
     2821 acgctgtatg ttaccggcga gcaggtgaaa tatcgtgatt cgcgcgaagg gatcgttcgt
     2881 gctaatcgga tcgtgatgaa tgatctgccg gatgggatca agacgatccg cgttacggaa
     2941 aatcgcctta acatgccgca ggtgacgacg gaaacggatg tcgccagcct gaaaaatcat
     3001 ctcgccggag agccgttggg ccacgaaacg aagctggcgc aaaaacgcgt cgagccagtg
     3061 gttccgaagt ccaccgagca gggctggtat atcgacaaat cgcgctttga tttccatatc
     3121 gatccggtgc tgaaccagtc ggtcggtggc ccggaaaact tttacatgta tcagctgggc
     3181 gtgatgggaa cggcagattt gtggctgacg gaccatctgc tgaccaccgg cagcctgttt
     3241 gcaaatcttg ccaacaacta cgacaagttt aactacacca atccgccgca ggactcgcac
     3301 ttaccgcgcg tgcgtaccca tgtgcgcgag tatgtgcaga acgatgtcta tgtgaataac
     3361 ctgcaagcca actacttcca gcatctgggc aacggcttct acggtcaggt ctacggaggt
     3421 tatctcgaaa ccatgtttgg cggtgcgggg gcagaagtgt tgtatcgccc gctggacagc
     3481 aactgggcgt ttggtctgga tgccaactac gttaaacagc gcgactggcg tagtgcaaag
     3541 gatatgatga aattcaccga ctacagcgtg aaaaccggac atctgaccgc ctactggacg
     3601 ccatctttcg ctcaggatgt gttagttaaa gccagcgtcg ggcagtatct ggcaggggat
     3661 aaaggcggca cgctggagat cgctaaacgc tttgacagcg gcgtggtggt gggcggctat
     3721 gccacgatca ccaacgtttc gaaagaggag tacggcgaag gggacttcac caaaggcgtg
     3781 tatgtctctg taccgttgga tctcttctcg tctggcccga cacgcagccg tgcggcgatt
     3841 ggctggacgc cgctgacgcg tgacggtggt cagcaacttg ggcgtaagtt ccagttgtat
     3901 gacatgacca gcgaccgtag cgtcaatttc cgctaagtca tgggaaaggt gccagttttc
     3961 gcaatcactg gcacctttat tcttaatggc ctgttgagcc atcaggaacg cagttcaccc
     4021 ccggtttgcg atctggtgtg taaaggcgaa aacattctgt cttcctgtcc gtcgatatcg
     4081 gttgcaggtc tggttgcctg agcgactggg ccatcattgg gctggagaca ttgctttgcg
     4141 gtaggtcggc tttatcagca gtaccgagcg gaccagcata agcaggaaga gcagagactg
     4201 ataacatcaa agcagcaaaa taaggcttca tttttaccac ctttatcagg ttacgtttca
     4261 tttgttccag aggaacattg tcgatttttc gcgcattgct ggtggctggg aatcacctga
     4321 atgggtgatt tttgaattac cggctttggt gcggtttgtc ttgccggatg cgccgccagg
     4381 cgcggcttat ccggcctacg ggtaggtata tccggctttg gtggaggcgc gctccaaatc
     4441 caggttgaac aaaacataca caaaaaatat agatctccgt cacatttttg cgttatacag
     4501 gaagctcgcc actgtgaagg aggtactgct atgacgtcac tctctcgtcc gcgcgtggag
     4561 tttatctcca ccattttgca gaccgtactc aatcttggcc tgctgtgcct cggccttatt
     4621 ttggttgtct tcctcggcaa agaaacggtg catctggctg atgtgctgtt cgcgccagaa
     4681 caagccagca aatatgagct ggtagaagga ctggtggttt actttctcta tttcgaattt
     4741 atcgcgctga ttgtgaagta ctttcagtcc ggttttcact tcccgctacg ttactttgtc
     4801 tatattggga tcaccgccat tgtgcgcttg atcatcgtcg atcataaatc gccactggat
     4861 gtgttgatct actcggctgc gatcctgtta ctggtggtca ccctgtggct gtgtaattcg
     4921 aaacggctga agcgggagta aaaagacagc acgccgaact ggcgcggcgt gctggacagg
     4981 aagattacag cgtagcagtt tgttgtgttt tcttcgtttc cggttcccag agggcttcca
     5041 gctcctcaag ggttttacct ttggtttccg ggacaaattt ccacataaac agtgctgcca
     5101 gaacgcccat acaaccgtaa atccagtagg agaaaccgtt gtggaaatgg gccaccagcc
     5161 aggagttttt gtccatcatc gggaaggtcc aggagacgaa gtagttcgcc agccactggg
     5221 ccgccaccgc gattgccagc gctttacctc gaatagcatt cgggaagatt tccgacagca
     5281 gtacccagca taccggaccc caggacatgg caaaggcggc aacatagaac agcatcgaca
     5341 gtagcgccac aatacccggt gcctgagtgt aaaacgcggt accgaggcta aacataccga
     5401 ttgccattcc gagtgcgccg ataatttgca gtggcttacg accaaattta tccaccgtca
     5461 taattgccag aacggtgaag gtgaggttga taactccgac aataatggtc tgcaacagcg
     5521 cgatatccgt gctggccccc agcgttttga acacttccgg cgcgtagtac agcaccacat
     5581 tgatgccgac aaattgctgg aagatggaga gcataacgcc gatgacaatc acgcccacgc
     5641 caaacatcag cagacgacca ccggttttgc ggccatgatc cagggagtgt ttaatttcct
     5701 gtactgcctg agttgcaagc gtgttgccca taattttgcg caggatacct tccgcctgtt
     5761 cttgcttgcc gcgtgacatc agccagcgag gactttctgg cacggtatac agcagcatta
     5821 agaacagcag tgcagggata cattccgagg caaacatata acgccagccg tcagtattca
     5881 gccagctggc atcaccggaa cgcgcaataa aatagtttac gcagtaaact aaaagctgcc
     5941 cgaaaataat cgcaaactgg ttaaatgaaa ccagtttccc gcgaatatga gcaggagcaa
     6001 gttccgcaat atacattggt gaaagcattg aggctaaacc aacgccaata ccgccaataa
     6061 tacgataaat aacaaattcc gggacataac ctgccagata aacaggcact gtgttgtccg
     6121 ggtttataga ggtaaaacca agttctggcc aggcagaacc tacaccagaa ataaaaaaca
     6181 ggacagcagc aatcttaagt gaatcacgac gaccgaagcg gttactgcaa taaccaccga
     6241 gggcaccgcc gatgatgcaa ccaatcagag cgctggccac gcaaaaccct aacagggagt
     6301 tggcagcgga ttcacttaag ttttgtggag caacaaagac ggtattgagt gactcaacag
     6361 taccggaaat aacggcggtg tcgtagccaa ataataaacc acctaatgta gcgactaagg
     6421 taatcgaaaa tatataactg gaattatact gggtattcat tcagacctgc cttagaccat
     6481 tctgatcttt tatcagtgca ttgatgttgg aattgtcagg cagttaaggc aattatcatt
     6541 ttttacaatg cgtttacgta atgtcttttc tgtgatctta attgtgataa ttatccaaaa
     6601 ttgataaaaa caatactatt gccgtgactc agagcacgaa agagaattat cgtaag
//
LOCUS       DAZKIP010000192         6503 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZKIP010000192 DAZKIP010000000
VERSION     DAZKIP010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.194"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..796
                     /locus_tag="ACHOUK_004482"
     CDS             <1..796
                     /locus_tag="ACHOUK_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFP9899792.1"
                     /translation="DLFIQRITHNLWTAREKNVTLIKQINDLKMCVNEYIGDCTDEEL
                     NDREFIASVVDRAIFHFAINSICNPEDNKDATLIERCTFDVETKNGLPSTVQLFYEES
                     KDNEPLANIHLQAIGSGFLTFVNACQEHDDNSLKLFASLLISLSYSSVYSDFAGRVNI
                     NEYNDNYLKAQFEELSQRDMKKYLGEMKRLADGGEMNFDGYLDKMSHLVNEGTLAPDI
                     LSKMRDAAPKLIDFAKSFDPNSKEKIKIYTGTSKLIYDLFGVKSEK"
     gene            1158..1262
                     /locus_tag="ACHOUK_004483"
                     /pseudo
     CDS             1158..1262
                     /locus_tag="ACHOUK_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
     gene            1409..1942
                     /locus_tag="ACHOUK_004484"
     CDS             1409..1942
                     /locus_tag="ACHOUK_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899793.1"
                     /translation="MLTLDEIGQSVRNNIQLIIDHVGLPLAVGPLSDDDYKILCGGYG
                     ELEWDYALSTYGNSREKYEFCIKLVQQGRVQGIPSGAAICVYGVEENIFRIHMIERFS
                     REDESHPLKGRMVLLTLMSAFIFCKAVECKVVHIVEPVPELVQYYESFGFRMEQCGYV
                     MSAVIDELQDIFLKFAQ"
     gene            2139..2312
                     /locus_tag="ACHOUK_004485"
     CDS             2139..2312
                     /locus_tag="ACHOUK_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899794.1"
                     /translation="MKDQKATKPQVKFDTMKAFAGMGAAVEVLMKAAPNAFTHATVSG
                     KEQQGKLRRRKAA"
     gene            complement(2360..2641)
                     /locus_tag="ACHOUK_004486"
     CDS             complement(2360..2641)
                     /locus_tag="ACHOUK_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyocin activator PrtN family protein"
                     /protein_id="HFP9899795.1"
                     /translation="MNTLFLLMAEFNTPNIELSAVSQKYFGMSPATAEAKANACKLPV
                     PTYRIGTSQKAKRCINIQDLAEYIDKRREEGRIEWEQVRTVKQKDKEHH"
     gene            complement(2678..3028)
                     /locus_tag="ACHOUK_004487"
                     /pseudo
     CDS             complement(2678..3028)
                     /locus_tag="ACHOUK_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
     gene            complement(2986..3183)
                     /locus_tag="ACHOUK_004488"
     CDS             complement(2986..3183)
                     /locus_tag="ACHOUK_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899796.1"
                     /translation="MAELTKWLQNTITGIETVVDDKSFVCDEIVFKIDVVKNVLTAFK
                     VALVSLEVLMILCRSAMLSQN"
     gene            complement(3330..3803)
                     /locus_tag="ACHOUK_004489"
                     /pseudo
     CDS             complement(3330..3803)
                     /locus_tag="ACHOUK_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145689.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
     gene            complement(3800..4150)
                     /locus_tag="ACHOUK_004490"
     CDS             complement(3800..4150)
                     /locus_tag="ACHOUK_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eae protein"
                     /protein_id="HFP9899797.1"
                     /translation="MRMNVFEMEGFLRGKCVPRDLKVNETNAEYLVRKFDEVRAEARN
                     EGINYTASRLAAAFNHGFINKPLAEVFDVTRMILSAKEELANESHPIDGLSGEYAEKS
                     LEEWAERLRKGGSQ"
     gene            complement(4141..4677)
                     /gene="yfdR"
                     /locus_tag="ACHOUK_004491"
     CDS             complement(4141..4677)
                     /gene="yfdR"
                     /locus_tag="ACHOUK_004491"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFP9899798.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIDINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFNVIPLAPSHA
                     YGMFMERFNELSELHKCA"
     gene            complement(4805..5221)
                     /locus_tag="ACHOUK_004492"
                     /pseudo
     CDS             complement(4805..5221)
                     /locus_tag="ACHOUK_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2303 family protein"
     gene            5209..5931
                     /locus_tag="ACHOUK_004493"
                     /pseudo
     CDS             5209..5931
                     /locus_tag="ACHOUK_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
     gene            5999..>6503
                     /locus_tag="ACHOUK_004494"
     CDS             5999..>6503
                     /locus_tag="ACHOUK_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP9899799.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAEYLHARTSAPG
                     SDNLNGINVKYRYEFTDTLGMVTSFSYAGDRNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNP"
BASE COUNT         1738 a         1405 c         1501 g         1859 t
ORIGIN      
        1 agatttattt attcaaagaa tcactcataa tttatggact gcacgtgaaa aaaatgtgac
       61 gttaattaag caaataaacg atctcaaaat gtgtgtcaat gagtatattg gagattgtac
      121 tgatgaggaa ttaaacgatc gtgaatttat tgcatcagtt gttgacaggg ctatttttca
      181 tttcgcgatt aatagtatat gtaatcccga ggataataaa gatgcgacac tcatagaacg
      241 atgtactttt gatgtagaaa ccaagaatgg tcttccctcc acggttcagc tattttatga
      301 ggaatctaaa gataatgaac ctttagcgaa tatacatttg caagcaatag gttctggttt
      361 cttaacgttt gttaatgcct gccaggaaca tgatgacaac agcttaaaat tatttgcttc
      421 gctgttaatt tccctttcat attctagtgt ctactcagat tttgcaggaa gagtgaatat
      481 taatgaatat aatgataact acctgaaagc tcagtttgaa gaattatctc aacgtgatat
      541 gaagaagtac ctgggagaga tgaagcgtct ggcagatggg ggagaaatga attttgatgg
      601 ctatctggat aagatgtcac atctggtgaa tgaaggaacg ctcgcccctg atattttaag
      661 caaaatgcga gatgctgcac cgaaattaat tgactttgct aagtcgtttg acccaaactc
      721 aaaggaaaag attaaaatat atacaggcac ttctaaatta atttatgatt tattcggggt
      781 taaatcggag aaataatatg tgaagttctt cgatggtatg aaaggtatta cataaaagaa
      841 cccaatgttt attgggttct ttttttctct atcagtgcta ttagcaggga gagatatcac
      901 cggagtttaa tgtgtgattt ttatttatcg tcgaatctgt attgtttatc attgcttttc
      961 acaacgctaa catctaataa gattagatat tcacttcgtg agagcttcat gccttgagag
     1021 gatctcaatt ttcttttgca atgagacagg cgcttcctgt tgttatggta tagtaccccg
     1081 ttattgagcc tcctgaacag tgatgctgaa taacataacc acatgatata tcgataaaat
     1141 aatctctaca tttgaaaatg cacggtaatt ctgaaatgca aaaaatcaac caaaccagcg
     1201 caatgcctga aaaaactgac gttcactgga gtggtcggtt tagcgttgca ccaatgctcg
     1261 ataggatgta ccgtttttga aaaacaaata gttatatact ttgtgggagc ctattgggaa
     1321 cccggcgttt tcatttcaag gtgtaatcca tacgcggctt aagaatgaga tataatgcga
     1381 cttttagtgt tccgcttgag aggccatgat gcttaccctg gacgagatag gtcaatcagt
     1441 acgtaacaat atccagttga ttattgatca tgtcggctta cctcttgctg ttggtccgct
     1501 cagtgatgat gattacaaga ttctgtgtgg tggctatggt gagcttgaat gggactatgc
     1561 gttaagtacc tatggcaact ccagagaaaa gtatgagttc tgcataaaac ttgttcagca
     1621 aggtcgggtt cagggaatac catcaggagc agcaatttgt gtttatgggg ttgaagaaaa
     1681 catctttcgt atccatatga tcgaaaggtt ttctagagaa gatgaatctc acccattgaa
     1741 agggcgcatg gttttactca ctcttatgag tgcttttata ttttgtaaag ctgttgaatg
     1801 taaagttgtc cacattgtag agccagtacc agaactggtg cagtattacg agtcttttgg
     1861 tttccgcatg gaacagtgcg gttatgtgat gtcggcagtc attgatgagc tgcaggatat
     1921 ctttcttaaa tttgctcagt aggtatagac gagaagggtc tacaaattgt aggatacccg
     1981 tccagattac cttaaaggta catctatggc agtcgttttg tgcttaaact actaagaaac
     2041 gatgtcacca atcgacatga tcgattggca taagttagcg aaacaagcta gctttaaaga
     2101 aagggttaga gacgccttta ctgtctcggg agttttctat gaaagatcaa aaagcaacca
     2161 agccacaggt taagttcgac acaatgaaag cattcgcagg tatgggtgct gctgttgaag
     2221 ttctgatgaa ggctgctcct aatgcgttca ctcacgctac tgtctctggt aaagagcagc
     2281 agggtaagct tcgtcgtcgc aaagcagcat gatcatagct ggtgcttttt gaaaacccgc
     2341 cttcaggcgg gttttttctt tagtgatgtt ctttgtcctt ctgtttgact gttctgacct
     2401 gttcccactc gatacgtcct tcttctcgcc ttttgtctat gtattccgca agatcttgaa
     2461 tattgatgca acgttttgct ttttgtgatg tgccgatgcg atatgttgga acgggcaact
     2521 tacaagcgtt tgcttttgct tctgccgtgg ctggactcat accaaagtac ttttggctaa
     2581 ctgctgagag ttcaatgttt ggggtattga attcagccat cagtaaaaac aaggtgttca
     2641 taattttctc catcaaaacc ggctgcaccc gggaaaatca taattctgtg ctggtggcag
     2701 gaattaattt ctgccagata gcggaaacat attttgcctg atgacgggca tcagccaggg
     2761 cgttgtgccg ttcgccatcg aaaggcatgt ccattttggg gtcgaatccg atggaacgcc
     2821 caagcgtaac gatcgtgcgt acatcgtggt cattccagta cgcccacggg cagatttgtc
     2881 ctgctcgctc gtaagctcca cgtaaaatta cgttgtcgaa ggtggccccg ttaccccaga
     2941 cttttaaata tttcgtattg tctgcgtgcc ggttaatgaa atggtttagt tctgagagag
     3001 catcgctgat cgacaaagta tcatcaatac ttccagcgat accagcgcga ctttaaatgc
     3061 ggtaagtacg tttttaacca catcgatttt gaatactatt tcatcacata caaacgattt
     3121 atcgtctact accgtttcaa ttccggtaat cgtgttttgt agccatttgg ttaattcagc
     3181 catttttcat taccgccctt tcgggcggtc tcctgatgtt ctgagggtgc aggaatccct
     3241 ccggttaagg attaaatttt atttacagaa ctgaatttaa ttattcagat atacgtatct
     3301 gtaaccttac gaacctactc actggatgcc tatttcataa aaataatcca gtgggtttta
     3361 tcgttttttc ctgttcgttg accgataaca ggttttctgt cggtcagtac caatatctgg
     3421 cgaacaggta tttgcgtttc attccattta aaaatcagaa cgccggatgg acgcaacaca
     3481 cgaaaggctt ctttaaatcc ctgccgcaaa tcatcacgct aggtatcttt attcagccgt
     3541 ccatatttct ttcccatcca ggcgttatca ccaacacgct caagatgcgg agggtcgaat
     3601 ataaccatcg aaaaagatgc gtctgcaaat ggtagtgcac gaaaatcagc tatcagatca
     3661 ggactgataa tcaggcgtcg tccatcacac aatgtgtgct cttcctttct gatatcgcta
     3721 aatatcgccc ggtcgtcatt cttatcgaac cagaacatgc gactgccaca gcacatgtcg
     3781 aggattgctg catgtccagt cactggctgc ctcctttgcg aagccgttcc gcccattctt
     3841 caagggattt ctctgcatat tcaccggaca ggccatcaat cggatgcgat tcattagcta
     3901 actcttcttt tgctgacaga atcatgcgtg taacgtcgaa aacttcagcc aaaggcttat
     3961 tgataaatcc gtgattgaaa gcagcagcaa gacggcttgc ggtatagtta ataccctcgt
     4021 tgcgagcctc agcacgtact tcatcgaatt tacgcaccag atactcagca tttgtttcat
     4081 tcactttcag atctcgcggt acacatttcc cgcgaagaaa cccttccatt tcgaaaacat
     4141 tcatgcgcat ttgtgtaact ccgataactc gttaaaacgt tccataaaca tcccgtaggc
     4201 atggctaggt gccagtggaa tcacgttgaa catctctgtt gccgggatgc cttccagtac
     4261 aggccagaaa gagccatcat caagcccgag atcgcggcgt tcggttgcca gcatgataag
     4321 atcggcatat ttcacgggcg tactcataac tgggggtaac ccgtatttct cacggattac
     4381 ggcgtcaatt ttttcttcca tccgtttata gtcaggaaga aggcgtttca gtggagcggg
     4441 gatgtcctgg caatacgctt ctgttgcatc atgcattaat gcttcaaaag caaattcctg
     4501 cggtaccagc tggctgcaaa gaaccgcatg ttgggcgacg ctgtagaagt gcgaaagatg
     4561 accggcaaag cgacagatat ttgaaaggga aaccgcgata tcgttaatat cgatgtcgtc
     4621 tttatttatc ctgtcataat aaaaatgctt cccggaaaaa gttttaataa atgacatttt
     4681 gttctccacg tatatgcgct gcaccgcgct gaattctggt aaaaagaatc cctcaccatc
     4741 cggcgattat tgagtaaatt acgtttccat aaatgccccc tcaggggcat ttgcagtaat
     4801 gaaatcaggc ggtgaaagta ccaataaagg tttctacttt gctgtccttg aatttctcaa
     4861 caagcagatc acgaaattcg ttagccattt cttcctgcac cgcctccagc tgaataatgc
     4921 gcagaaccag taccggacga tcgccagtga taatgctgag gcgtaattta aacggacgtt
     4981 ctttcagacc ttcaaacgga atgcatttaa attcaaatgc cactggcata atgtctttgg
     5041 tcttcgcttc gacagactcc atcagggagc gtttgccgct gaagtcatta tcttcaaaat
     5101 cagcggtctg gtttgcttca atcgtgattt tacggaccgc cgcagccgct tttgttgcct
     5161 gaatggtgtc accattagca tcaaagccca caaggtagtc ggcccagtct tcaatccatt
     5221 ctggttgccg ccaatcgtat cgcgtttatt gacccggcaa acgggaatga aacgccgatg
     5281 tttgtggcgc agggcaacca gatattcatg aacgacgtgt tcctgaagcg cctgacggcc
     5341 cccaccatta ccagtggtgg aaatccaccg gcattttccc tgacaccgga tggaaagctg
     5401 actgctaaaa atgcggatat cagtggtaac gtgaatgcga actccgggac gctcaacaac
     5461 gtcacgatta acgagaactg tcgggttctg ggaaaactgt ccgcgaacca gattgaaggc
     5521 gatctcgtta aaacagtggg caaagctttc ccccgggact cccgtgcacc ggaacggtgg
     5581 ccatcaggga ccattaccgt cagggtttat gacgatcagc cgtttgaccg gcagattgtt
     5641 attccggcgg tggcattcag tggcgctaag catgagagag agcatactga tatttactcc
     5701 tcatgccgtc tgatagtgcg gaaaaacggt gctgaaattt ataaccgtac cgcgctggat
     5761 aatacgctga tttacagtgg cgttattgat atgcctgccg gtcacggtca catgacgctg
     5821 gagttttcgg tgtcagcatg gctggtaaat gactggtatc ccacagcaag tatcagcgat
     5881 ttgctggttg tggtgatgaa gaaagccacc gcaggcatca gtatcagctg aattttataa
     5941 cccatatacg ggcgccagaa atggcgcctt ttttattgca gaaaagcgag aggtaattat
     6001 gcgtaaactt tatgccgcca ttttgtccgc agccatctgt ctggccgtat ccggcgcgcc
     6061 tgcatgggcg tctgaacatc agtccacgct gagcgcggag taccttcatg cccggacgag
     6121 cgctcccggt agcgataatc ttaacgggat taacgtgaaa taccgttatg agtttacgga
     6181 cacactgggg atggtgacgt cattcagcta tgcaggagac aggaatcgcc agcttacccg
     6241 ttacagcgat acccgctggc atgaagattc cgtgcgtaac cgctggttca gcgtgatggc
     6301 ggggccctct gtgcgcgtga atgaatggtt cagcgcgtat gcgatggcgg gtgtggctta
     6361 cagccgtgtg tcgactttct ccggggatta tctccgcgta actgacaaca aggggaaaac
     6421 gcatgatgtg ctgaccggaa gtgatgacgg tcgccacagc aacacgtctc tggcgtgggg
     6481 agctggcgtg cagtttaacc cga
//
LOCUS       DAZKIP010000193         6432 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZKIP010000193 DAZKIP010000000
VERSION     DAZKIP010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.195"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(52..336)
                     /locus_tag="ACHOUK_004495"
     CDS             complement(52..336)
                     /locus_tag="ACHOUK_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFP9899800.1"
                     /translation="MEAKECKVQDILTENKKFIIPSYQRPYSWTVDNAEQLIDDIYKS
                     SQSEENGYFIGSMICINKGQNQYEVVDGQQRLTTLSIIVSELKKSSRFRG"
     gene            614..3046
                     /gene="hsdR"
                     /locus_tag="ACHOUK_004496"
     CDS             614..3046
                     /gene="hsdR"
                     /locus_tag="ACHOUK_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HFP9899801.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPEQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVKDTDDVGPLPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVRKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            3113..4582
                     /locus_tag="ACHOUK_004497"
     CDS             3113..4582
                     /locus_tag="ACHOUK_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFP9899802.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSRDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPAELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4582..6336
                     /locus_tag="ACHOUK_004498"
     CDS             4582..6336
                     /locus_tag="ACHOUK_004498"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFP9899803.1"
                     /translation="MSVEKLIVDHMKTWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASELLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVRISEIGHDWGQKTPDKDFTYIDVGSINKEYGIIEELSILSAKDAPSRARKIV
                     QQGTIIYSTVRPYLLNIAIIENEILPEPIASTAFAIIHPYTAMDANFIYYYLRSPVFV
                     CYVENCQTGVAYPAINDKQFFSGITPVPPSLEQVRIANKIKELMSLCDQLEQQSLTSL
                     DAHQQLVETLLGTLTDSQTAEELAENWARISEYFDTLFTTEASVDALKQTILQLAVMG
                     KLVPQDPNDEPASELLKRIAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCL
                     FEDIIDIQSGITKGRNLSNRTLVKVPYLRVANVQRGYLDLTEIKQIEIPIEEKEKYQV
                     VKGDLLITEGGDWDTVGRTTVWCHDWYIANQNHVFKGRNIGQDVDPYWLETYMNSPFS
                     RQYFANASKQTTNLASINKTQLRGCPVAIPPSSEAKKIMSKLHIFYKLCEELKNHIQS
                     AQQTQLHLADALTDAAVN"
BASE COUNT         1944 a         1498 c         1450 g         1540 t
ORIGIN      
        1 tttcatcaga ataaacatca ataggcagaa ctcttttttg caaatcatct tttatcccct
       61 gaatcgggat gattttttta gctcggagac aataatgctt aacgttgtaa gacgttgttg
      121 tccatctaca acttcatact gattttgccc tttattgata caaatcatac tcccaataaa
      181 atatccattc tcttctgatt gagagctttt ataaatgtca tcaatgagtt gctctgcatt
      241 atctaccgtc caactgtaag gacgctgata agaaggaata ataaactttt tgttttcagt
      301 aaggatatcc tgcaccttac attctttagc ttccatttca tatacccaat caaacaatca
      361 acaataacat tatctttctt gctcgaaaga aacgcaacta taagattttt atatattttg
      421 ttagagttga tatggttcct gtaacttctg tttatctgtc tctgatatgc ccagattaat
      481 tcaagcaaga tataacagag atatcaatgt gatctctgct acgtacttta cgttgaaaca
      541 ccgccctaaa tcatagccag gctattgatt ttatgtacaa tcagaatcgt cctgataaaa
      601 gcgagaatac cccatggcgg aactgaacct aagtaacctg acggaagcag acatcattac
      661 caaatgcgtt atgccagcca ttctcaatgc aggctgggac aacacaacgc aaatcagaca
      721 ggaggtcaaa ctccgggacg gtaaagtcat cgtacgtggt aaagttgcgg cacgcagaac
      781 ggtaaaatct gctgacatcg tgctgtatca caaacctggc attcccttag ccgtgattga
      841 agcaaaagcc aacaaacatg aaattggcaa agggatgcaa cagggcattg aatatgcgcg
      901 cctgctggac gttccttttg tttttgccac caacggtgat ggctttatct tccgcgatgc
      961 caccgcagcc gaaggtgaat gccttgaaaa gcaaataacg ctggatgact tcccctcccc
     1021 cgctgaactc tggcaaaaat tctgcctctg gaaaggatac acacaagctc agcttccggt
     1081 gattactcag gactattacg acgatggcag cggtaaatcg ccacgttatt accagcttca
     1141 ggcaatcaac aaaaccattg aagccgtctc caacgggcaa aaccgcgttc tgctggtcat
     1201 ggcgaccgga acagggaaaa cctataccgc attccagatc atctggcgcc tgtggaaatc
     1261 aaaaaataaa aaacgcattt tgttccttgc cgatcgcaat attctggtcg accaaaccaa
     1321 aaataatgat ttccagccat ttggtacggc aatgaccaaa gtcagcggac gcaccattga
     1381 tcccgcttat gaaattcacc tcgcgctcta tcaggctata actggcccgg aggaagacca
     1441 aaaagcgttt aaacaagtcg caccagattt cttcgatctg atcgtgatcg acgaatgcca
     1501 tcgcggcagc gcatccgaag acagcgcctg gcgagaaatc cttgattatt ttagttccgc
     1561 cacccaaatt ggcttaaccg ccacgccaaa agagacgcat gaagtctcca gcacggatta
     1621 cttcggcgat ccggtttacg tctactcact aaaagaaggg atcgaagacg gcttcctcgc
     1681 cccttataaa gttgtccgtg ttgatattga tgttgatctg caaggctggc gcccaaccaa
     1741 agggcaaact gacttaaacg gcgaagtgat cgacgatcgt atctataacc agaaagattt
     1801 cgatcgcacg atggtaatcg acgaacgcac agaactggtt gccagaacca ttaccgacta
     1861 cctcaagcgt accaatccga tggataaaac catcgtcttc tgtaacgaca tcgatcatgc
     1921 agaacgtatg cgccgcgccc tggttaatct caacccggag caggtgaaaa agaacgacaa
     1981 atacgtcatg aaaatcaccg gcgatgatga aattggcaaa gctcagttgg ataacttcat
     2041 caacccgaaa aaaccgtacc cggttatcgc gaccacttca gagctgatga ccaccggtgt
     2101 ggatgctaaa acctgcaaac tggtagtact ggaccagaac atccagtcga tgaccaaatt
     2161 caagcagatt atcggtcgtg gtacacgcat cgacgaacgt tacggcaaac tctggtttac
     2221 catcctcgac tttaaaaaag ccaccgaact gtttgccgat gagcgtttcg atggcattcc
     2281 cgaaaaagtc atggatacca caccagagga tatcgccgat ccagaatctg attttgaaga
     2341 gaaactcgaa gaaatcagcg aacatgacga cgaacaggta acaggcgttg atgaaccgcc
     2401 tgcgccacca taccaggtta aagataccga tgatgtcggc ccacttccgg aagaagacga
     2461 gaagaaaatc cgcaagtttc acgtcaacgg tgtagcagtg ggcgttattg cccagcgtgt
     2521 tcagtattac gacgccgacg gtaaactggt taccgaatcc tttaaagatt acacccgcaa
     2581 aacactgctc aaagaatatg cctcgctgga tgactttacc cgcaagtggc aggacgccga
     2641 tcgcaaagaa gcgatcattc acgagctgga gcaacagggg atcatctggg aagtactggc
     2701 agaagaagtc ggtaaagatc tcgacccgtt cgacatgctt tgccacgtag tgtatggtca
     2761 gccgccgtta acccgcaaag agcgcgccga gaacgtgcgc aagcggaact acttcacaaa
     2821 atactctgaa gcagcgcaag ccgtgctcga taatctgctg gataaatacg ccgatgcggg
     2881 cgtacaggag atcgaaagta ttcaggtgct gaaacttaag ccattcgaca gcatgggcac
     2941 cttaccggag attattaaaa ccggatttgg cgaccgtaac gggtataatc aggcgctcag
     3001 cgagctggaa aacgaaatct accaattacc gccccgctct gcttaacaac tctcttttgc
     3061 gccttctgca accatcatgt tgcagaaggc cttgtttttc atggaataca acatgtctat
     3121 cagctcagta atcaaatcat tacaagatat tatgcgcaaa gatgccggtg tggatggcga
     3181 tgcgcagcgt ctcggtcagc tctcctggct gctgtttttg aaaatcttcg atgcccagga
     3241 agaggcgctg gaactggagc aggataacta tcaatatccg atcccacagc gttatttatg
     3301 gcgcagttgg gccgccaacg cgcagggcat taccggtgat tccctgctgg aattcgttaa
     3361 tgatgatctg ttcccggcgt tgaaaaacct cactgcgcct atcgataaaa acccacgcgg
     3421 ctacgtggtg aagcaggcgt tcagcgatgc ctataactat atgaaaaacg gtacgctact
     3481 gcgtcaggtg atcaataagc tgaacgaaat tgactttacc agcgccagcg aacgccatct
     3541 gtttggtgat atttacgaac agatccttaa agatctgcaa tctgcgggca atgcgggcga
     3601 attctatact ccacgcgccg tcactcgctt tatggtggat cgcgttgatc cgaaactcgg
     3661 cgaatccatt atggacccgg cctgcggtac gggcggtttt cttgcctgcg catttgatca
     3721 tgtaaagaac aaatacgtga agagcgtcgc cgatcatcag acgctgcaac aacagatcca
     3781 cggtgttgag aaaaaacagc ttccgcacct gctggcgacc accaatatgc tgctacacgg
     3841 cattgaagtg ccagtgcaaa ttcgtcacga caacactctg aacaaaccgc tttcctcccg
     3901 ggatgagcaa ctggatgtca ttgttaccaa cccgccgttt ggtggcacgg aagaagacgg
     3961 tattgagaag aactttccgg cagagatgca aacccgcgaa acggcggatt tgttcctgca
     4021 actgattgtg gaagtactgg cgaaaaatgg tcgcgcggcg gtggtattgc cggatggcac
     4081 actatttggc gaaggcgtta aaaccaaaat caaaaagcta cttaccgaag agtgcaacct
     4141 gcataccatc gtgcgcttac cgaatggtgt gtttaacccc tataccggca ttaaaaccaa
     4201 cctgttgttc tttaccaaag gtcagccaac caaagagatt tggttctatg agcatccgta
     4261 tccggcgggc gtaaaaaact acagcaaaac taagccgatg aagtttgaag agtttcaggc
     4321 ggagatcgac tggtggggta acgaggccga tggttttgcc agccgcgtag agaatgagca
     4381 ggcgtggaaa gtcagcattg atgacgtgat tgcgcgtaac ttcaatctgg atattaaaaa
     4441 cccacatcag gcggaaaccg tcagccatga tccggccgag ctgttagcgc agtatgcaaa
     4501 acagcaggcg gagatccaga cgctgcgtaa tcaactgcgc gatattcttg gcgctgcgct
     4561 gtctggcaag gaggttaact aatgagtgtg gaaaagctga tcgttgatca tatgaaaacc
     4621 tggacctcgg cgttgcaaac ccgttccacc gccgggcgcg gcagttccgg aaaaattgat
     4681 ttatatggca ttaagaaatt acgtgagctg attctggaac tggctgtgcg cggtaaactg
     4741 gtgccgcagg acccgaacga tgaaccggcg tcggagctgc tgaagcgtat tgcggcagaa
     4801 aaagcagagc tggtgaagca aggtaaaatt aaaaagcaaa aaccactgcc ggaaattagt
     4861 gaggaagaga agccgtttga attgccggag ggatgggagt gggtgcgtat tagtgaaatt
     4921 ggacatgact ggggacaaaa aactccagat aaagatttta cttacattga tgttggttca
     4981 ataaataaag aatatggaat tattgaagag ctatctattc tttcagcgaa agatgcccca
     5041 tcgcgagcga gaaaaattgt tcagcaaggg actatcatat actctactgt tcgcccgtat
     5101 ttgttaaata tagctattat tgaaaacgaa atactacctg aaccgatcgc tagtactgcc
     5161 ttcgctatta ttcatccata tacggcgatg gatgctaatt tcatttatta ttatctacgt
     5221 tctccggtat ttgtttgtta tgttgaaaat tgtcagacag gggttgccta tccagcaatc
     5281 aatgacaaac aatttttttc tggaataact ccagttcctc catccttgga acaagttcgc
     5341 atcgcaaaca aaatcaaaga attaatgtcc ctctgcgacc aactggaaca gcaatccctg
     5401 accagtctgg acgcacatca acaactggtt gaaaccctgt tgggaacgct tacagacagc
     5461 caaaccgccg aggaactggc tgaaaactgg gcgcgtatta gcgagtattt cgacactcta
     5521 tttaccaccg aagccagcgt ggatgcgtta aaacagacca ttctgcaact ggccgtaatg
     5581 ggcaaacttg tgccgcagga tccgaatgat gaaccagcct ctgaactgct caaacgaatt
     5641 gcgcaggaaa aagctcaact ggtgaaagaa ggaaaaataa aaaaacaaaa accgttgccg
     5701 ccaattagcg atgaggaaaa accgtttgaa cttccggaag ggtgggagtg gtgtttattt
     5761 gaagatatta ttgatattca aagtggtatc actaaaggaa gaaatttatc aaatagaact
     5821 ttggtaaaag ttccttattt acgtgttgca aacgtccaac gcggatatct tgatcttacg
     5881 gaaattaaac agattgaaat ccctattgag gaaaaagaaa aatatcaagt agtcaaggga
     5941 gatttattga taacagaagg cggcgactgg gatacagtcg ggagaactac agtatggtgt
     6001 catgactggt atatagcaaa tcaaaaccat gtattcaaag gacgaaatat agggcaagat
     6061 gttgatccat attggttaga aacatatatg aatagcccat tctcaagaca atattttgct
     6121 aacgcaagta agcaaaccac taatttagct tctattaata aaacccagct cagaggttgt
     6181 cctgttgcta ttcctcctag ctcagaagca aaaaaaataa tgagtaaact acatattttt
     6241 tataaactat gtgaagaatt aaagaatcat atccaatccg cccagcaaac ccaactacac
     6301 cttgcagatg cactcactga cgcggcggta aactaattta cctgccctcg aaaaaccaat
     6361 aaagcggcta aatagccgct ttattcacat caccaaaatt tatatttcac gctaattttc
     6421 atcttatttt ca
//
LOCUS       DAZKIP010000194         6358 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZKIP010000194 DAZKIP010000000
VERSION     DAZKIP010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.196"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..2409
                     /locus_tag="ACHOUK_004499"
     CDS             <1..2409
                     /locus_tag="ACHOUK_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFP9899804.1"
                     /translation="VAVATAIEAGDERRARAKFHWQFLEHYPAAQDCAYKFLVCEDKP
                     GIPRPALDSWDAEYMQENRWDEASASFVPVETESDPMNVTFDNLAPEVQNAVMVKFDT
                     CENITVDMVISAQELLQEDMATFDGHIVEALMKMPEVNAMYPELKLHAIGWVKHKCKP
                     GAKWPEIQAEMRIWKKRREGERKEAGKYTSVVDLARARANQQHTENSTGKINPVIAAI
                     HREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWAKNEVIDNGREDWKRISASMRK
                     QPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITEYLTTKGVFEDEGTNQSATDTLS
                     SPVPETDAVETAIPDNEKTECKVEVEPSVEREGPFYFLFTDKDGEKYGRANKLSGLDK
                     ALAAGATEITKEEYFARKNGTYTGLPQNANTAQNSEQPEPVKVTADEVKKIMQAANIS
                     QPDAEELLAVSRGEFVEGISDPNDPKWVKGIQTRDSVNQNQQETEQNDQKAEQNSPNT
                     QQNEPETKQPEPVVQQEPEKICTACGQSGGGNCPDCGAVMGDATYQEIFDGENQPEVQ
                     ENDPEEMEGTAHQHKENTGGNQHHASDSETGEASDPLIKANGHHNLTSTSRAGIHLMI
                     DLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTIDLDTAGGVIDRDTMKWWLKQSR
                     EAQSAIMTDEIPLDDALLQLREFIDENSGEFFVHVWGNGANFDNTILRRSYERQGSPC
                     PWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNALDDARYQAKYVSAIWQKLIPS
                     QADS"
     gene            2482..2733
                     /locus_tag="ACHOUK_004500"
     CDS             2482..2733
                     /locus_tag="ACHOUK_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFP9899805.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRRHLT"
     gene            2768..2955
                     /locus_tag="ACHOUK_004501"
                     /pseudo
     CDS             2768..2955
                     /locus_tag="ACHOUK_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
     gene            complement(3430..3516)
                     /locus_tag="ACHOUK_004502"
                     /pseudo
     CDS             complement(3430..3516)
                     /locus_tag="ACHOUK_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3722..4048)
                     /gene="ynfA"
                     /locus_tag="ACHOUK_004503"
     CDS             complement(3722..4048)
                     /gene="ynfA"
                     /locus_tag="ACHOUK_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFP9899806.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGPLIALCGMLIIV
                     VGWGRT"
     gene            4183..4524
                     /gene="ynfB"
                     /locus_tag="ACHOUK_004504"
     CDS             4183..4524
                     /gene="ynfB"
                     /locus_tag="ACHOUK_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFP9899807.1"
                     /translation="MKITLSKRIDLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTGNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            4559..5119
                     /gene="speG"
                     /locus_tag="ACHOUK_004505"
     CDS             4559..5119
                     /gene="speG"
                     /locus_tag="ACHOUK_004505"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFP9899808.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(5122..5832)
                     /gene="ynfC"
                     /locus_tag="ACHOUK_004506"
     CDS             complement(5122..5832)
                     /gene="ynfC"
                     /locus_tag="ACHOUK_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFP9899809.1"
                     /translation="MKYKLLPCLLAILLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVGCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            5940..6245
                     /gene="ynfD"
                     /locus_tag="ACHOUK_004507"
     CDS             5940..6245
                     /gene="ynfD"
                     /locus_tag="ACHOUK_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFP9899810.1"
                     /translation="MKLSTCYAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
BASE COUNT         1897 a         1516 c         1459 g         1486 t
ORIGIN      
        1 gtcgctgtag ccactgccat tgaagccggt gatgaacgtc gcgcccgcgc aaaatttcac
       61 tggcaattcc tggaacatta tccggctgct caggactgcg cttataaatt tcttgtttgc
      121 gaggataaac ccggtatacc ccgccctgcc ctcgattcct gggatgctga atatatgcag
      181 gaaaaccgct gggatgaggc gtctgcttcc tttgtcccgg tcgagactga atccgatccg
      241 atgaacgtca cttttgacaa cctggcccct gaagtacaga acgctgtcat ggttaagttc
      301 gacacatgtg aaaacatcac tgttgatatg gtgattagcg cacaggaatt gttgcaggaa
      361 gacatggcaa cattcgacgg ccatatcgtt gaagcgttga tgaaaatgcc agaagttaac
      421 gctatgtatc cggagcttaa gctgcatgcc atcgggtggg ttaagcataa atgtaagcct
      481 ggtgccaaat ggcccgaaat tcaggcagag atgcgcatct ggaaaaaacg tcgcgaaggt
      541 gaacgcaagg aagccggaaa atacacgtct gttgttgatc tcgcccgcgc cagagccaat
      601 caacagcaca ctgaaaattc aacaggaaaa atcaacccgg tcattgctgc cattcatcgc
      661 gaatacaagc agacatggaa aacactggat gacgaactgg cctacgctct ctggcctggt
      721 gatgtggatg ccggaaacat tgacggcagc atccatcgct gggcaaaaaa tgaagttatc
      781 gacaacggcc gcgaagactg gaagcgtatc tcggcatcaa tgcgcaaaca gcctgatgcc
      841 cttcgctacg accgccagac tatttttggc cttgtccgtg aacgtccgat cgacattcac
      901 aaagaccctg tggcactgaa caaatacatt actgaatacc tgactacaaa gggcgtgttt
      961 gaagatgaag gaacaaatca gagcgcaact gatactctct cgtcgccagt accagaaact
     1021 gatgcagtgg aaacggcaat tccagacaac gaaaaaaccg aatgcaaagt ggaagtcgaa
     1081 ccatctgtag aacgtgaggg gccgttctac ttcctcttca ccgacaagga tggcgaaaaa
     1141 tacggtcgcg caaacaaact ttctggtctg gataaggcgc tggctgccgg ggctactgaa
     1201 atcacaaaag aagaatattt tgcccgaaaa aatggcacat acacaggctt accgcaaaat
     1261 gcaaataccg cacaaaattc tgaacaacca gaaccggtaa aagttaccgc tgacgaagta
     1321 aagaaaatta tgcaggcagc caatatcagc cagcctgacg ccgaggaact gcttgccgta
     1381 tcacgtggtg aatttgttga agggattagc gacccgaatg atccgaaatg ggttaagggg
     1441 atccagaccc gcgattctgt gaaccagaac cagcaagaaa cggaacagaa cgaccagaaa
     1501 gcggaacaaa acagcccaaa tacgcaacaa aacgagccag aaacgaaaca acctgaacca
     1561 gtagtgcaac aggaaccgga aaagatctgc accgcctgcg gtcagagcgg tggcggcaac
     1621 tgccctgatt gtggtgcggt gatgggtgac gcaacatacc aggaaatatt cgatggagag
     1681 aatcagcctg aagttcagga aaatgatccg gaggaaatgg aaggtactgc acatcagcac
     1741 aaggagaaca ctggcggcaa tcagcatcat gccagcgata gtgaaactgg cgaggcgtca
     1801 gatcccttaa ttaaggcgaa cggtcatcat aatctcacat ccaccagcag agcggggatt
     1861 catctgatga tcgatcttga aaccatggga aaaaatcccg atgccccgat tatctcaata
     1921 ggtgcaatat ttttcgatcc gcaaaccgga gatatgggac cggaatttag taagactatc
     1981 gatctggata ctgctggcgg agtcattgat cgggacacca tgaaatggtg gcttaaacaa
     2041 tcacgcgaag cgcaatctgc cattatgacc gatgaaatcc cgttagatga tgcactgtta
     2101 caattgcggg aatttatcga cgaaaactcc ggtgaatttt ttgttcatgt ctggggaaat
     2161 ggagccaact tcgacaacac gattttgcgc cgttcatacg aacggcaggg gagcccctgc
     2221 ccgtggcgtt actacaacga tcgcgatgta cgcacaatcg ttgagctggg gaaagccata
     2281 gacttcgatg ccagaacggc tattccattc gaaggtgagc gccataatgc acttgatgac
     2341 gcccgttacc aggcaaaata cgtttcagct atctggcaaa aactgatccc gagtcaggct
     2401 gattcttaat gttcaactgt cgccggttgt gactggtatt ctgcaaccgg cgctcgtctg
     2461 atgtaagaga taaagaaatc gatgagcgaa gtaatcatga ttgtctctcc cggcaaatgg
     2521 gtatccgaag agcagttaat tgcgctgaaa ggaataaaaa aaggtacgtt aaaaaaggcc
     2581 cgggaaaaat cgtttatgga aggaagggaa tataagcatg tcgctcatga cggtatgcca
     2641 tgggataaca gtccatgctt ttacaacctg gaagaaattg atcgctggat tgagcgccag
     2701 gcatcagcaa gaccaagacg tcatcttact tgactaaaag ccacactaac taatgagaga
     2761 agttgaaatg aaatatccga caggcgtgga aaaccatgga gggaaattac gtatctggtt
     2821 tgtttataaa ggcgtaagag tcagggaaaa cctggggttc ctgacacagc aaaaaacagg
     2881 cgcgttgcag gtgagctacg cgcctctgtt tgttacgcaa taaaaactgg tgttttcgac
     2941 tatgcaaaac agtttccctc ctcacgcaat ctggaaaaat ttggtgaggc ccgacaagat
     3001 ttaaccataa aagaactggc tgaaaaattt ctggcactga aagaaactga agtcgcaaaa
     3061 acatcactca acacataccg tgccgtcatc aaaaatatcc tgagcataat cggtgaaaaa
     3121 aatcttgcct catcgattaa taaagaaaaa ttactggagg ttcgtaaaga gctactgact
     3181 ggataccaga tccccaaaag taactatatt gttacacaac cagggagatc ggctgtaact
     3241 gtaaataatt acatgacaaa tcttaacgcc gtgttccagt ttggtgttga taacggttac
     3301 ctggcagata acccgtttaa ggggatctcg ccattaaagg aatcaagaac cattccggat
     3361 cctctttcgc gggaggaatt catccgtctt atcgatgcgt gcagaaatca gcaagccaaa
     3421 aatttatggt gtaataccgt cctcagtggt gatttttaat gtgacgaaat tacgccccgg
     3481 acaggtaaca aaaacttcag cctttacgat cttcatgttc gattccttgc atcgcttgtc
     3541 gtgatgcatg aaatctacgc aactgagcta ctaccatata agtatgaaga tcgaaaaaag
     3601 ccggagtgat cacaaaaaaa ggcgtatatt tgcgctgtga atggttgaca aaagatgaaa
     3661 tagaatacct tttgtcagct gacacttcct cgtatcttat tgataaaatg gatttatgtt
     3721 cctacgtgcg cccccagccc acaacaatga tcaacatgcc gcaaagcgca atcaacggcc
     3781 ccgtccagtc ataaagagtc agtttcacgc catccacaac gcgcagccac atcaacgccg
     3841 tgcagacata aacgccacca taagccgcgt aaacacgccc actcgccgca ggatgcaacg
     3901 ttaacaacca gacaaacagc gccagtgaaa tccccgccgg aagcaacagc cagatactgg
     3961 cgtttcgttt taaccacaac cagggcagaa agcatccaat aatttcacac agcgcagtag
     4021 caaaaaatag taacgttgtt ttaatcatct ttgtcactta ttgacatcat gtatagttat
     4081 agggcgacat aatatcatca atataaacac cctcctggta cgttttgcgt tcgcagtgga
     4141 tggtgtagaa tcatctttaa tcattcatac agggaatgaa ttatgaaaat cactctcagc
     4201 aaacgaatcg acctgctcgc ttttctgctg ccttgcgcac tggcattgag cacaactgtt
     4261 catgccgaaa ctaacaaact ggtgattgag tctggcgaca gtgcacaaag ccgccagcac
     4321 gccgctatgg aaaaagagca atggaatgac acgggcaatc tgcgccagaa agtgaataaa
     4381 cgcactgaaa aagagtggga taaagccgac gccgcttttg ataaccgcga taaatgcgag
     4441 caaagcgcca acatcaatgc ctactgggag ccaaatactt tgcgctgcct ggaccgtcga
     4501 actggccgcg ttattacccc ctaacctgtt attgatttaa ggaatgtaag gacacgttat
     4561 gccaagcgcc cacagtgtta agctacgccc gctggagcgt gaagatttac gctatgtaca
     4621 tcaactcgac aataacgcca gtgtgatgcg ttactggttt gaggaaccct acgaagcctt
     4681 tgttgaactc tctgatctgt atgataagca tattcacgat cagagcgaac gacgctttgt
     4741 ggtggaatgt gacggcgaaa aagccggtct ggtggagttg gtggaaatta accatgtaca
     4801 tcgccgcgca gaatttcaga taattatctc cccggagtat caggggaaag gtctggcaac
     4861 ccgtgccgcc aaattagcaa tggactatgg cttcaccgtt ctcaatctct ataagctgta
     4921 tctgatcgtt gataaagaga atgaaaaagc gattcacatt taccgcaagc ttggcttttc
     4981 ggttgaaggt gaattgatgc acgagttctt tattaatggt caatatcgta atgccattcg
     5041 catgtgtata ttccagcatc agtatctggc agagcacaaa acaccgggtc agactctcct
     5101 gaagccgacc gcacaatagc attaataata atcgatcgta tttttgatgg tgtaaacccg
     5161 ttcgacggcg ggttttactc cttcatcaac aatgatcagc tgacagccca ccggattggc
     5221 gtgactgtca tattcacagc tctgttttac attaccaact cgttgattat tcagtaaagt
     5281 aaccgctgtg taatctaatt ttttgatcgg gtccgttgat ggcgtggcgc tgacagataa
     5341 tgttttgtcg ttacttttcg tggttttacc cagtggataa ccctgatcat catagcgata
     5401 ttccatctgc atttgtttgc tgctggcttt aatcacgaag ccattatcat cggtttccca
     5461 actcaccccg gcagaaggta attctgctag ctggcatttt ccctgtaaac gtactctctt
     5521 ctccagcgtc tcggcatcac ggtaataatt ggcgtccagt accagagcga ccacggtatt
     5581 attttccaga tccagtaatt cgagtgaatc aaaacagcct tcttccgaca aagtccccga
     5641 aacacgtttc gtcacttcac cttgctcatc cattaatgtc tgagtgaaat cttttaccgg
     5701 accacgcagc ggatcaaaat cgaattcatt agagaaactg gccatctcag gagtaaatga
     5761 aagtgttacc tctgtgcggt cacatcctgt gagtaatatc gcgagtaagc atggcagtaa
     5821 tttgtatttc acatcagtca ccagagagta gagatgattc tcaatcatag tagcaaatac
     5881 agtactttac acgtgaaatg ctatgcttaa agaagttatc ttcgcgttag gagcttatga
     5941 tgaaactctc aacctgttat gccgcgcttc tgctcgccct cgcctcaccc gcggtactcg
     6001 ccgcgccggg atcctgtgag cgcatacaaa gcgatatatc acagcgcatt atcaataatg
     6061 gcgtacccga aagcagcttc acgttaagta ttgtacccaa tgaccaagtt gatcagcctg
     6121 attcccaggt cgtcgggcat tgcgctaatg atacgcataa aattctctac acccgcacca
     6181 ccagcggtaa cgtctctgct cccgcgcagt ctacccagga tggcgcgcct gccgaaccgc
     6241 agtaatacat tatcgactac acgccggata cgacacatcc ggcgatttgg acatacaaca
     6301 taatcccacc atattactca tacccttcta ttgatatgga ttaataatta ttaacccc
//
LOCUS       DAZKIP010000195         6333 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZKIP010000195 DAZKIP010000000
VERSION     DAZKIP010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.197"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            179..358
                     /gene="yciG"
                     /locus_tag="ACHOUK_004508"
     CDS             179..358
                     /gene="yciG"
                     /locus_tag="ACHOUK_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFP9899811.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            444..944
                     /gene="yciF"
                     /locus_tag="ACHOUK_004509"
     CDS             444..944
                     /gene="yciF"
                     /locus_tag="ACHOUK_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFP9899812.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            990..1496
                     /gene="yciE"
                     /locus_tag="ACHOUK_004510"
     CDS             990..1496
                     /gene="yciE"
                     /locus_tag="ACHOUK_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFP9899813.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(1556..1681)
                     /locus_tag="ACHOUK_004511"
                     /pseudo
     CDS             complement(1556..1681)
                     /locus_tag="ACHOUK_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203849.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
     gene            1905..2006
                     /locus_tag="ACHOUK_004512"
                     /pseudo
     CDS             1905..2006
                     /locus_tag="ACHOUK_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2416..2651)
                     /locus_tag="ACHOUK_004513"
                     /pseudo
     CDS             complement(2416..2651)
                     /locus_tag="ACHOUK_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2833..3459)
                     /locus_tag="ACHOUK_004514"
     CDS             complement(2833..3459)
                     /locus_tag="ACHOUK_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001116930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFP9899814.1"
                     /translation="MPFSFNLSSGNYLSTQDVEVLQRATRDHQMERLTIGERSFSVRY
                     QSAMDAFIVDPVQGELYSGLSHTELADIIRLADSVENQLNGGNSFLDVFSTYMGQVIS
                     EFMHSNDNRIELLQRRLHSCSFLVNIEEMSYIDEALQCPITLAIPQRGVFLRNAEGSR
                     VCSLYDEMALSRIINDGMHHPLSREPITLSMLVAREQCEFDCSIGHLR"
     gene            3691..4747
                     /locus_tag="ACHOUK_004515"
                     /pseudo
     CDS             3691..4747
                     /locus_tag="ACHOUK_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(4966..5556)
                     /gene="espM1"
                     /locus_tag="ACHOUK_004516"
     CDS             complement(4966..5556)
                     /gene="espM1"
                     /locus_tag="ACHOUK_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector guanine nucleotide exchange factor
                     EspM1"
                     /protein_id="HFP9899815.1"
                     /translation="MPVNATGVSFSSFGISYHKDNSFRGTIRGKNDEVVKCSMGERSI
                     RFNVNKFSGCILETVSRQSTKDIHGWVSDERTVYPSRVINQEIDNCCLQKNAKISSEE
                     RKMVFSLVSKEFELTLDVKAAQSSINHIIIGNASFGKKMDALCDGMSRAVKNSTTDYI
                     ANVLADKFYQKHIAPGVDIVKLRNEIPGYMSRVIQG"
     gene            5740..>6333
                     /gene="nleG"
                     /locus_tag="ACHOUK_004517"
     CDS             5740..>6333
                     /gene="nleG"
                     /locus_tag="ACHOUK_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase NleG"
                     /protein_id="HFP9899816.1"
                     /translation="MPVILNFSSERVLSESELEALRHVGRVSQSEQLVVRGRTMRLHH
                     ISFMDSFSVEPVSGGLLDRLSARGHRLLAENLEIQLNRGHTFLQAFRLYMEQSRATPC
                     TRQNVSSAIQNKINSHAFTVSHQDFSCHEQHLNCPITLCIPETGVFVRNAKNSEICSL
                     YDHNALTELIRRNAPHPLSREPFVPEMIVSKDECHFNL"
BASE COUNT         1960 a         1397 c         1217 g         1759 t
ORIGIN      
        1 taagtcactt attccgcagg caattgttaa tatatcaaga acgttcgtca taatatattt
       61 tcctactttc ttctcgttac gcttaatttg actaatcctc attagcgact aattttaatg
      121 agtatcgaca cacaacactc atattaatga aacaatgcaa cgcaacggga gaaataacat
      181 ggccgaacat cgtggtggtt caggaaattt cgccgaagac cgtgagaagg catccgacgc
      241 aggccgtaaa ggcggtcagc atagcggcgg taattttaaa aatgatccgc aacgcgcatc
      301 tgaagcgggt aaaaaaggcg gtcaacaaag cggtggtaat aaatcaggaa aatcctgatt
      361 caccgttaat tattaatatc agctgataaa gataattgca tctgcgggcc gtatggctcg
      421 cagtgatttc actggagaaa aatatgaata tgaagaccat tgaagatgta tttattcacc
      481 tgctttcaga tacctacagc gcagaaaaac aattaacccg ggcactggca aaactcgcaa
      541 gagcaacatc aaacgaaaaa ttaagtcagg cttttcatgc gcacctcgag gaaacacatg
      601 gacagattga acgtattgat caagttgtgg aatcagaatc gaatctgaaa attaagcgca
      661 tgaaatgtgt ggcaatggaa ggtcttattg aagaagctaa tgaggtcatc gaaagtaccg
      721 agaaaaacga agtgcgtgat gccgcactga ttgccgcagc acagaaagtc gagcattatg
      781 agattgccag ttacgggaca ttagcgacgc tggctgaaca attaggttac cgtaaagcag
      841 cgaagcttct gaaagagacc ctggaagaag aaaaggccac cgacatcaaa ctgactgatc
      901 tggcccttaa taacgtaaat aagaaagccg aaaataaagc ctgaaatatg aactttaact
      961 tttggtcatt ttataaagag gacattttca tgaatcgtat tgaacattat catgactggt
     1021 tacgtgacgc ccatgcaatg gaaaagcaag ccgaatctat gcttgaatcc atggccagcc
     1081 gtatagataa ttatcctgaa ctacgtgctc gtattgaaca acatcttagt gaaaccaaaa
     1141 accagattgt tcaactggaa actattcttg atcgtaatga catttcacgt tcagtcatta
     1201 aagattccat gagtaaaatg gctgcgcttg ggcagtcaat cggtggtata ttcccttctg
     1261 atgaaatagt caaaggctct attagcggat atgtcttcga gcaatttgaa atcgcctgtt
     1321 acacctctct attagcagca gcaaaaaatg ccggtgatac agcctcaatt ccgatcatcg
     1381 aagcgatttt aaatgaggaa aagcaaatgg ccgactggct gattcagcat attccgcaaa
     1441 caactgagaa atttttaatt cgctctgaaa ctgatggcgt agaagcgaag aaataatacc
     1501 ctttatgcca tgtccttatt gaccccgtat attacggggt cgtttttgtg cggaattaaa
     1561 aacgatatcc tgctgagaac ataaacaccc acggatcgag gcgtacgcta tcatgttgct
     1621 gtgcaccgcc cagtttataa ttggcggtgg tatcgatatc catgtaccac actggtagat
     1681 tttacaaaac tcaataacat attatattta aaggaaaaat acagaattta cgatgctttt
     1741 ttatacagat ctctcgtgtt tttaactgat tgattttgtt gaactctttt tttgttttgc
     1801 aggagttact cctcttacct tattgtccac tcgatgacca acacattcac aacccaaacc
     1861 agaatatcct atctatgaag catgtatccg cacgcagatt aattatggtt tacattgttc
     1921 taccacaata gtcgcatcct aaatgggtgc ggttgaggat caccatcctt gaagaggatg
     1981 cccgccactt taactgcatg acagggctat ttaaacaatc actaaaaatc gcccaagagc
     2041 attctgtttc tataatgtat tccaagagat taaaaaataa tatcatacgt tattcggcag
     2101 agttcgtatg tactacggcg tcccaaccat gtcccaaaac aaaaatttct gccctataca
     2161 cgttccaaaa aattcaacag caaagagagt ctgctgacga gttgttgatt cactgattaa
     2221 tacgtaaaat tgacaactgc tggaaatcat acaatactcg tactttcgaa agttcgctaa
     2281 ccaggtgcta cacgcgaggt gttacagttt tctttacgat ttatcattca atcctaatcc
     2341 atgcatgaca tgtggtgcct gtggggtgat cctacctacg taatgtggac acagccctaa
     2401 tcaaggttct gtttttcaaa acagttccgt gctgagacag ccacaagtac tgtgtcggca
     2461 ccaccgattg taatcacact agatctaatt aaacacagcc gtctgcatta tttcccgact
     2521 cataaaatat tagccgtaaa tgcattcgac ttttagtgag tggaagaaac tttccgccct
     2581 agcattatcg taacagcgcc cctttgcact catcctgtca tgcaggtatg ccgcttcaga
     2641 agcgcctgat aatgcgttga aatcaggacg gtggctactt gctattcagt tcacgataca
     2701 tcccttaagg accagcgaat agcaatgagt cactttatta tcaagtttga tgaccgcttt
     2761 gatgctaact tttaataaga gtctcttcac acaaaatcgt gtaccgagtc tacactgaat
     2821 aacaatcact cctcaccgta agtgaccgat actgcaatca aactcacact gctctctggc
     2881 cacaagcatt gataatgtta ttggctctct gcttagtggg tgatgcatcc catcattaat
     2941 tatacgagaa agagccattt catcatataa actacatact ctggaacctt cagcatttct
     3001 taaaaaaaca cctcgttgag gaattgccag cgtaatcggg cactgtaatg cttcatctat
     3061 gtaagacatt tcttcaatat taactaaaaa tgaacatgaa tgtaatcgcc gctgtaacaa
     3121 ttcaattctg ttatcattac tatgcataaa ttcagaaata acctgcccca tatatgtact
     3181 gaatacatca agaaatgaat tgcctccatt caattgattt tcaacagaat cagccaatct
     3241 aatgatatcg gctagttctg tatggcttaa acccgaatat agttcccctt gaacaggatc
     3301 tacaataaaa gcgtccatag cactctgata gcgaacagaa aacgatcttt ctcctattgt
     3361 gagtctttcc atttgatgat ctctggtcgc acgttgtaaa acttcaacat cttgtgttga
     3421 caagtaattt ccagaagaca aattaaatga aaatggcata attttatccc taaagcatat
     3481 atcttacttt tcgataatta acaaaaagcc atcctccaca gcaatactcc atcagaataa
     3541 ttgtaggatt attttaatct aatttataag cataaaatat atactcacta aatagctgcg
     3601 cagaatagta gatcatttag agggaactca acccgattgt gcgatctgat caatcgccaa
     3661 atcaatacaa atcaccaacc ggactgagca atgtcgatca taccaccaat ttcccgtgac
     3721 gaacgacgcc taatacagaa agccatccat aaaaggctga cagccatgct gatgctgcac
     3781 cggggcgacc gtgtcagcga cgttgcccgc acactatgct gcgcccgttc ctctgttggg
     3841 cgctggatta actagttcac acagtcgggt gttgagggac tgaaatcatt acctgccgag
     3901 cgagcccgcc gctggccgtt tgcgcatatc tacacactat tacgtgaaca ggtaaaatat
     3961 tcactcgcga ctttggctac aattattcac gctggagtac agaactgctg acaataaaaa
     4021 tcaatgagat aaccggctgc ctgttacatg ctggaaccat tcgccgctgg ttgtcgtctg
     4081 tggggcttgt gtggcgaagg gctgcgccaa ctctacgtat ccgtgccccg cctaaagata
     4141 aaaagatggc aacaatccat aacgcactgg acgaatgcag cacagagcat ccggtctttt
     4201 atgaagatga agtatttatc catcttaatc ccaaaatcgg tgcggactgg aaactgctcg
     4261 ggaagcaaaa acgaggggtc acgccggaac aaaatgaaaa atattctctg gacgtagcgc
     4321 tgcacagcgg gacaggttaa gtcagctgtg tgggcgccaa cagcaaaagt tcggcgctgt
     4381 tcatcagact gctgaagcgg cttaaagcta catactgtcg gacgaaaacc atcacgctgc
     4441 tcgaggacaa ctacattatc cacaaaggcc gggaaaccca gcgctggctg aaggataaac
     4501 cgaagtgcag agtcatttat cagccggttt aatctccatg ggtgaatcat tttgaatagc
     4561 tatggcagat acttcacgcc acaataacgc gtaatcatca gtgccgctca atgtggcaac
     4621 tattgaaaaa gttcgccatt ttatggaatc cgtcagtcca ttccccgaag gcaaacatgg
     4681 gctggcaaaa atgtgcgata ttaggcgcag ctatttagtt attccgtcat ttctcccgcc
     4741 cgtgtaaaca ctactgaatc ttgtcgttgt gacaaagcta taaatagtca atcacagcac
     4801 gtttctgcgt acgacgcgct gcaattttct ttacgattta taactgtaca ggtgaactat
     4861 gagttatttg gatacataca tatagtgtaa cgcctgttgt atattttact catatctttt
     4921 tacaggatga aaaccaatga tactggcgca tagcctcctc caaccttacc cctgtataac
     4981 acgactcata taacctggga tttcatttcg tagttttaca atatcaacac ccggggcgat
     5041 atgtttctga taaaacttgt ctgcaagcac gtttgctatg taatctgttg tactgttttt
     5101 tacagctcgg ctcataccat cgcaaagagc atccattttt ttgccaaaag aagcatttcc
     5161 tattattata tgattaatag aactttgtgc cgctttaaca tcaagagtta gttcaaactc
     5221 cttacttaca agagaaaaga ccattttcct ctcttcagaa gaaatttttg catttttttg
     5281 aaggcagcaa ttatcaatct cttggttgat aacccttgac ggatatactg tccgttcatc
     5341 acttacccat ccatgaatat ctttagtact ctgcctgctt accgtctcca gtatacagcc
     5401 gctaaatttg ttaacattaa agcggataga gcgctctccc attgaacact tcacgacctc
     5461 atcattcttc ccacggatgg tgccccggaa agaattatct ttatgataac tgataccaaa
     5521 agagctgaag gacacacctg tcgcatttac tggcataaca aatccttttc attaaaattt
     5581 gcttacaaaa attgtataca atcattttaa ttaaaaaaat acatgtgtaa atagcattcg
     5641 ttcaaaattt aattttttgt ttgaatccta tcacatattt agtatcttga ctcctgtcac
     5701 caatatggtt caataacata gactaaagag ggagaaaata tgcctgtcat attaaacttt
     5761 tcgagtgaaa gagtattgtc tgaaagtgaa ctggaggctt tacgtcatgt cggaagagtt
     5821 agtcagagtg aacagcttgt tgtgagaggc cgcactatga gacttcatca tatttcattt
     5881 atggacagtt ttagcgtgga accggtttcc ggcggactgt tggatcgcct tagtgcaaga
     5941 gggcatcgcc ttctcgcaga aaatctggag atacagctta acagaggcca tacttttttg
     6001 caggcattcc gtttatatat ggaacagagc cgtgcaaccc catgtacgcg ccaaaacgtc
     6061 agtagtgcaa tacaaaacaa aattaactct catgcattta ctgttagtca tcaagacttt
     6121 tcctgtcatg aacaacacct gaattgcccg ataacattat gcattcctga aactggtgtt
     6181 tttgtcagaa atgcaaaaaa ctcagaaata tgttcattat atgaccacaa tgcactcact
     6241 gaacttatcc gacgcaacgc tccacaccct ctcagccgtg aaccctttgt tccggaaatg
     6301 attgtcagca aagatgaatg ccattttaat cta
//
LOCUS       DAZKIP010000196         6166 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZKIP010000196 DAZKIP010000000
VERSION     DAZKIP010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6166
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.198"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            196..330
                     /locus_tag="ACHOUK_004518"
     CDS             196..330
                     /locus_tag="ACHOUK_004518"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899817.1"
                     /translation="MPHPERNHCLLLMGYLHGVQASELLGIKLSDIDLQAGNLNIRRL
                     "
     gene            932..1753
                     /locus_tag="ACHOUK_004519"
     CDS             932..1753
                     /locus_tag="ACHOUK_004519"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFP9899818.1"
                     /translation="MLNARHLPVLMYHHVSRCPGLVTLSPETFCEQMKWLADNNWRTV
                     TSAELEYFYQGGTLPRKSVMLTFDDGYLDNWLRAWPVLKEYNLHAHIFLITGLIGKGN
                     VRSRQEQEYSHRDCERLIADNRSDEVMLRWSEVREMRDSGLVEFHVHTHSHKRWDRLS
                     VSRAEQCRLMKEDILVGKQCLTEKLGFCSSHLCWPEGYYNRDYINLAGKLGFSYLYTT
                     ERRMNCPENGSLRIGRISTKEREHSGWLKRRLFYYTTPLFSSVLALHKGPRLPDN"
     gene            1753..2859
                     /locus_tag="ACHOUK_004520"
     CDS             1753..2859
                     /locus_tag="ACHOUK_004520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP9899819.1"
                     /translation="MMKILFTESSSDIGGQELQALAQMTALQKQGHSVLLACREKSKI
                     APEARKRGHDVTFIPFRNSLHLPSILRLRRIIGEFKPDLVICHSGHDSNIAGLSRLIC
                     CHRFSIVRQKTYITRKTRTFSLNYLCDFIVVPSSAMMAHLMAEGVRTPVTVIPPGFDW
                     PALHNEAMRPLPLHIHAWAASADNVPLIVQVGMLRPEKGHEFMLRVLYQLKMEGKSFR
                     WLVVGAGREEYEARLRQQTEHLGMSGDVLMAGALFPALPVYRIASVVVMPSENEAFGM
                     VLAEASVSGVPVIASETGGIPDVIQKNVTGTLLPVGDVSAWTGALRDFLSRPERFRMM
                     AASAREDIEYRFDINRTAQIIVSLASQAKGKCNR"
     gene            2949..4670
                     /gene="cptA"
                     /locus_tag="ACHOUK_004521"
     CDS             2949..4670
                     /gene="cptA"
                     /locus_tag="ACHOUK_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFP9899820.1"
                     /translation="MHLNTGQNRPTFSWSALGWAIFYFGFFSTLLQVIIFSSGYSGTN
                     GIRDSLLFSCLWLIPVFLYPDRIKIIAAVVGFILWGTSLAALCYYFLYGHEFSQSVLF
                     VMFETNAREAGEYFSQYFSLKLLLISLVYTAVSVFLWTRLRPVYIPLPWRRIVSFLLL
                     YALLLHPVVLKSLIRQEPLNDTLGKLASRMEPAAPWQFVSSYYQYHQQLNALTTFLNE
                     NSALPPLGNLRDESGERPRTLVLVIGESTQRERMSLYGYLRETTPELDALRKTDPGLT
                     VFNNVVASRPYTIEALQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTIT
                     ARNTMLTVFSRQTDRQYYMNQQRTQSAREYDTNVLKPFREVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPEGQGRFDGITGHIPTGLNAKELEVYNDYDNANLFNDHVVASLIKDFRAT
                     APDGFLLYFSDHGEEVYDTPPYKTQGRNEDNPTRPMYTVPFLLWTSEKWHAAHPRDFS
                     QYVDRKYSLAELIHTWSDLAGLTYDGYDPTRSLVNPQFRETTRWIGNPYKKNGLTDFD
                     TLPYGEP"
     gene            4744..5742
                     /gene="lpxM"
                     /locus_tag="ACHOUK_004522"
                     /gene_synonym="msbB"
     CDS             4744..5742
                     /gene="lpxM"
                     /locus_tag="ACHOUK_004522"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052689.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFP9899821.1"
                     /translation="MVPPSAVLCYHNEISRQIPVNMKNIRTEFIPRFNLTLCFPRYWM
                     TWTGIGIICVFAMVPPALRDPLLGKLGMLVGRLGKSARQRALINLSLCFPEYSDKEKE
                     NIVDAMFATASMAVVLMAELALSGPDKISHRIRWNGLEIVEKMAQNNEKVIFLVPHAW
                     GVDIPAMLMAASGRKMAAMFHNQRNPVVDYVWNSVRRRFGGKLHARNDGIASFVRSVR
                     QGYWGYYLPDQDHGPEFSEFADFFATYKATLPVIGRLSRISGARIIPLFPVYDGKTHH
                     LTIHVSPPLAIRQKSDAHIARQINEVVENFVRPHPEQYTWILKLLKTRKEGEEDPY"
     gene            5795..5983
                     /locus_tag="ACHOUK_004523"
                     /pseudo
     CDS             5795..5983
                     /locus_tag="ACHOUK_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767718.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1595 a         1336 c         1547 g         1688 t
ORIGIN      
        1 catgaatgtt tgcaaaaaaa ataaatgaac aataatttta tgtatttaaa ataaattgat
       61 tttacttctg taatggtgtc atgattagca gctttttaaa ctaatatcga ctttcaataa
      121 ataaggacag gtaatggaca acaaaaatat ctgaacccgg gtgagattca taagttgcac
      181 agtgctgtct tcaaagtgcc acacccggaa cgtaatcact gcctgctcct tatggggtat
      241 cttcatggtg tccaagcatc agagttatta ggtataaaac tgtctgatat tgatttgcag
      301 gccggtaacc tgaatatccg gcggctctaa acggtttgtc aactcagccc ctcggctgaa
      361 acaggaggta cagtccattc gtcgctggct gaaacttcgg gatgaatggg tagaagaggg
      421 atatgaatgg ttatttaacc tctatggcag gtctgctaca ggtattactg ttgttctggg
      481 gggcactggt gtggttaata tctacgatgc aagagattct cacgatggcc cggtccatca
      541 gtgcatgcgg cgcgatattg tgcgagtatt tttcaggggg agtggtatcc ggatgcagaa
      601 aattccgcta ctcgactggt tttatgcaat aaatcaggat gataatcttc tgatttttta
      661 ctgaggcgga tatctctcgc tgtgtttact gaaattgcgg tattctgttt tttctgaaaa
      721 acagaaaatc tgattaaaaa gagcatatgc gttaagggaa atttaagttc tggttgatta
      781 actgataaaa atattaaaaa atccctgttg tcggtaatct ccggaccatt ccgggattac
      841 acaataatat caccatgtgt gatatctatg gatcctcact gttgtttacg cgaggatatt
      901 ttcagaatgc ttaacagacg attatttttt tatgttgaat gcaaggcatc ttcccgtact
      961 gatgtatcat catgtcagtc gttgtccggg gcttgtgaca ctctcacctg aaacattttg
     1021 tgaacagatg aagtggctcg cagataataa ctggcggacg gtaacatctg ctgagcttga
     1081 atatttttat caggggggaa cgcttccacg aaagagtgtc atgctcacat tcgatgatgg
     1141 ttatcttgat aactggctca gagcatggcc ggtcctgaag gaatataacc ttcatgcaca
     1201 tatttttctc attacggggc tgattggtaa gggaaacgta cgtagcaggc aggagcagga
     1261 atattctcac cgcgactgtg aacggttgat tgccgataac cgttcagatg aggtcatgct
     1321 tcgctggtct gaagtccggg aaatgcgtga ttccggactg gttgaatttc atgtgcacac
     1381 ccattcacat aaacgctggg acaggttgtc tgtatcacgt gctgaacagt gccggctcat
     1441 gaaagaagat attctggtgg gaaaacaatg tctgacggaa aagctggggt tctgtagttc
     1501 ccatctgtgc tggcctgaag gatattataa tcgggattat attaatctcg ccggtaagct
     1561 tggtttctct tatttatata caaccgaaag aagaatgaac tgtcctgaaa atggttcatt
     1621 acgcataggg cgtatcagca ccaaagagcg ggaacattcc ggctggctga aacgacgcct
     1681 tttttattat acaacgccac ttttttcttc agtactggct cttcataagg ggccaagatt
     1741 accagataat taatgatgaa aattttattc actgaatctt cgtcagatat tgggggccag
     1801 gagctgcagg cactggccca gatgactgct cttcaaaagc agggacattc cgttttactt
     1861 gcctgcagag aaaaaagtaa aattgctccg gaagccagaa aaagagggca tgatgtcact
     1921 tttattcctt tcaggaacag ccttcacctt ccttccatcc tcagacttcg acggattatc
     1981 ggggaattta aacctgatct ggtcatctgc catagtggcc atgacagtaa tatagcagga
     2041 ctgagccgtc tgatatgctg tcaccgcttc agtattgtca gacaaaaaac ctacatcacc
     2101 cgtaagacca gaacgttttc cctgaattat ctgtgtgatt tcattgttgt gcctagctca
     2161 gccatgatgg cgcatctgat ggcggaaggt gtcaggacac cggtaacggt tattccaccg
     2221 ggtttcgact ggcccgcatt gcataatgaa gccatgcgac cgttacccct gcatatccac
     2281 gcctgggctg catcagcaga caatgttccg cttatcgttc aggtggggat gctacgtccc
     2341 gaaaaaggac atgaatttat gctgcgtgta ttgtatcagc tgaagatgga agggaaatca
     2401 tttcgctggc tcgtggtggg cgccgggcgt gaagaatacg aagcacgtct gcgtcagcag
     2461 acagaacacc tgggaatgag cggagatgtg ttaatggcag gtgccctttt cccagcgtta
     2521 ccggtctacc ggattgccag tgtggtggtc atgccttcgg aaaatgaagc gtttggtatg
     2581 gtactggctg aagcgtcagt ctccggcgtg cccgttatcg ccagtgagac gggcgggatc
     2641 cctgatgtca ttcagaagaa tgtgacaggg acgctgttac ccgtggggga tgtgtctgca
     2701 tggaccggcg cactgcgcga ttttttatcc cgaccggaac gcttccggat gatggcagcc
     2761 agcgccagag aagatattga gtaccggttt gatattaaca gaacggcaca gatcattgtg
     2821 tctctggcta gtcaggctaa ggggaaatgc aacagataac ggcggatcct cttttcagcc
     2881 cgaattttgt tcagcggttt tccggtaacg gagtaattta tcacattgtt taaatacggg
     2941 gacggtcaat gcatttaaat accggacaga acaggcctac tttcagctgg agcgcgctgg
     3001 gatgggcaat tttttatttt ggcttttttt ccactctcct gcaggtcatc atcttcagca
     3061 gtgggtacag cggaacgaat ggaatacggg actcactgtt attcagttgt ctgtggttga
     3121 tcccggtgtt tctctatcct gatcggataa aaataattgc agctgttgtc ggtttcattc
     3181 tctggggcac gtcgctggca gcactttgtt attattttct ctatggtcat gaattctctc
     3241 aaagtgttct tttcgttatg tttgaaacga atgccagaga ggctggtgaa tatttcagcc
     3301 agtatttcag ccttaaactg ttgcttatct cgctggtata tactgcggtg tccgtttttc
     3361 tgtggacacg tctgcgtcct gtatatattc ctttgccatg gcggagaatt gtctctttcc
     3421 tgctgcttta tgctctgctt ctgcatccgg ttgttctgaa atcgttaatc agacaggagc
     3481 cgctgaatga tactctaggc aaactggcat cccgaatgga gcctgctgcc ccctggcagt
     3541 ttgtatccag ttattatcag taccatcagc aactgaatgc actgacaacc ttcctgaatg
     3601 aaaatagcgc actgccacca ctgggtaatc tcagggatga atcaggggag agaccacgca
     3661 cactggtcct ggtgattggt gagtcgacac agcgcgaacg catgagctta tacgggtatc
     3721 tacgtgaaac gacgccggag ctggatgcac tgcgtaaaac cgatccgggt cttactgtgt
     3781 ttaataatgt ggtggcatcg cgtccgtaca ccattgaagc attgcaacag gcccttactt
     3841 tcgccaacga aaagaatcct gatctgtatc tgacgcagcc gtcgctgatg aacatgatga
     3901 agcaggcagg ctataaaaca ttctggatta ccaaccagca gacaatcaca gcccgtaaca
     3961 ccatgctcac tgtattttcg cgccagacgg acaggcagta ctacatgaat cagcagcgaa
     4021 cacaaagtgc gcgtgaatat gacactaacg tgctgaagcc gttccgggaa gtgctgaatg
     4081 accctgcacc aaagaaactg atcatcgtgc atctgctggg tacgcacatt aagtataaat
     4141 accgttatcc ggaaggtcag gggcggtttg atggcattac agggcatatt cccactggat
     4201 taaatgcgaa agagctggaa gtgtataacg attacgataa tgccaatctg tttaacgatc
     4261 atgtggtggc cagtctgata aaggacttca gagcaactgc gccggacggc tttctgcttt
     4321 atttttcaga ccatggtgaa gaagtatatg atactccgcc atataaaacg cagggacgga
     4381 atgaagataa ccccacacgt cccatgtaca ctgttccgtt cctgctgtgg acctcggaaa
     4441 agtggcatgc tgcacatccg cgagattttt cgcagtatgt tgaccgcaaa tacagtctgg
     4501 ctgaactgat ccacacctgg tcagatttgg cgggactgac atacgatggt tacgatccga
     4561 cccgttctct ggtgaatccg caattcaggg aaaccacccg ctggattgga aatccgtata
     4621 aaaagaatgg gctcactgat ttcgacactc ttccgtatgg tgagccatag tattttctta
     4681 tcttcggttc cgttctgaag gcaggaggat gtgttttcct ctgctgtccg gtggcctcca
     4741 gggatggtac caccttctgc tgtactctgt tatcataatg agataagcag gcagataccg
     4801 gtaaatatga aaaatatcag aactgagttt attccccgct ttaaccttac attatgcttc
     4861 ccccgttact ggatgacgtg gacaggtatc ggtattatct gtgtgtttgc aatggtcccc
     4921 cccgcactga gagatccatt gttgggaaaa ttgggcatgc tggttgggcg tctggggaaa
     4981 agcgcacgtc aacgggcctt gattaattta tcgctttgtt ttccggagta cagtgataaa
     5041 gaaaaggaga atatcgttga tgccatgttt gcaacagcct ccatggccgt tgtgcttatg
     5101 gctgaattag cactgtccgg gcctgataaa atttcgcatc gtatacgctg gaatggtctg
     5161 gagattgtgg agaagatggc acagaacaat gaaaaggtca tttttcttgt gccacatgcc
     5221 tggggagtgg atattcccgc aatgctgatg gctgcgtcgg gcagaaaaat ggcagcgatg
     5281 tttcataacc agagaaatcc ggtggtggat tatgtctgga acagtgttcg tcgtcgtttc
     5341 ggaggaaaac tgcacgcacg taatgacggt attgcctcct ttgtccgctc agtccggcag
     5401 ggatactggg gatattatct tcctgatcag gatcatggcc cggaattcag tgagtttgca
     5461 gatttttttg cgacgtataa agcaacttta ccggttatcg gacggctcag caggatcagt
     5521 ggtgcacgga taataccgct tttccctgtt tatgacggaa agacccatca tctgactatt
     5581 catgtcagcc cgccacttgc tatacgacag aagagtgatg ctcatattgc gcgacagata
     5641 aacgaagtgg ttgaaaattt cgttcgtcca catcctgagc agtatacgtg gatcctcaag
     5701 ttgctgaaaa cgcgtaaaga aggggaagag gatccttact gaggcagctt tttcaggata
     5761 atgagcttac ccagcaatag tggacacacg ctaagtgagt aaactctcag tcagaggtgg
     5821 ctcatatgac aaaaaaacag taccaaccag taaaaaaccc cgtaaatagc attcgcctga
     5881 atttcgcagt gaagccctga agcttgctga acgcatcggt gttgctgtcg cagctcgtgt
     5941 gctcagtctg tatgaatcac agcttgtaag cggctcgtca gaactgtatt gatatttact
     6001 gagcggtcag ttcaacgtaa gcgagtgtta tgcgccatgg aagcttaccc aggaatatgt
     6061 tatcaggcct cagcatggcg aagtgttgca acgctggaag cagaatgacc gtaagcgtac
     6121 agcctgaacc gtctggtcag aatctgacga attagacaaa gtggtg
//
LOCUS       DAZKIP010000197         6086 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZKIP010000197 DAZKIP010000000
VERSION     DAZKIP010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6086
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.199"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            258..776
                     /locus_tag="ACHOUK_004524"
     CDS             258..776
                     /locus_tag="ACHOUK_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFP9899822.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            986..3126
                     /locus_tag="ACHOUK_004525"
                     /pseudo
     CDS             986..3126
                     /locus_tag="ACHOUK_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
     gene            3202..>6086
                     /locus_tag="ACHOUK_004526"
     CDS             3202..>6086
                     /locus_tag="ACHOUK_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFP9899823.1"
                     /translation="MGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGIT
                     YANPVNPVLGAKVLPGETDLALPGPLPFILSRAYSSYRTRTPAPVGVFGPGWKAPFDI
                     RLQIRDEGLILNDNGGRSIHFEPLFPGEISYSRSESFWLARGGVAEQHSSQPLSALWQ
                     VLPEDVRLSPHMYLATNSLQGPWWILNWPERVPGADEVLPPEPPAYRVLTGVVDGFGR
                     TLAFHRAAEGDVAGAVTGVTDGAGRRFHLVLTTQAQRAEVFRKQRATSLSSPAGPRSA
                     SSSLVFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDELPAAPLARYTYTASGELRAV
                     YDRSGTQVRGFAYDAEHAGRMVAHHYAGRPESRYRYDDTGRVTELVNPEGLDYRFEYG
                     QDRVTITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDAAGRR
                     TEYSLHMASGAVTAVTGPDGRTVRYGYNSQRQVTSVTYPDGLRSSREYDEKGRLAAET
                     SRSGETTRYSYDDPASELPTGIQDATGSTKQMAWSRYGQLLTFTDCSGYTTRYEYDRY
                     GQQIAVHREEGISTYSSYNPRGQLVSQKDAQGRETRYEYSAAGDLTAIVAPDGSRSEI
                     QYDAWGKAVSTTQGGLTRSMGYDAAGRITVLTNENGSQSTFRYDPVDRLTEQRGFDGR
                     TQRYHYDLTGKLTQSEDEGLITLWHYDASDRITHRTVNGDPAEQWQYDEHGWLTTLSH
                     TSEGHRVSVHYGYDDKGRLTGERQTVENPETGEMLWEHETGHAYSEQGLATRQEPDGL
                     PPVEWLTYGSGYLAGMKLGGTPLVEYMRDRLHRETARSFGGEAYELATAWNTSGQLRS
                     RHLNLPQLDRDYDWNDNGQLIRISGPQESREYRYSDTGRLTGVHTTAANLDIDIPYAT
                     DPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYIYRYDEYGRLAEKTDR"
BASE COUNT         1400 a         1606 c         1951 g         1129 t
ORIGIN      
        1 ggccatttta tggaggcaat acggactttg aacagttggg tatggctatc cagatatata
       61 ttattaagtt attgctgaat aagagattta atgattttaa aaatagtttt tacgacaacc
      121 gtaaattcta gctcggtttg tatgtaaaaa atactgaaaa atgatgcggt tccgtgaagt
      181 gagctgcatc tgcctgtctg ggattaacgg caggtcattg taagtagtaa ttaaccacgt
      241 taacaaggag tttacaaatg ccaacaccgt gttatatctc catcactggt cagacccagg
      301 gaaatattac tgcaggtgca tttaccgctg attctgtcgg caacatctat gtgcaggggc
      361 acgaagacga gatgctggtg caggaattcc tgcataacgt tactgtcccg accgatcctc
      421 agtccggtca gcctgcgggt cagcgtgctc acaagccttt catttttacc gtggcgctga
      481 acaaagcggt tccgctgatg tacaacgcac tggcctccgg cgaaatgctg ccaaccactg
      541 aacttcactg gtggcgtacc tctgtggaag gcaaacagga gcattatttc actacccgcc
      601 tgactgattc gacgattgtc gatatgaaac tgcatatgcc gcattgccag gacccggcga
      661 aacgcgagtt cacccagttg ctcgaagttt cactggccta ccgcaaaatt gaatgggaac
      721 acgtcaaatc tggcacctcc ggtgccgacg actggcgcgc accgctggaa gcataagtta
      781 agccaacagc atccggctat caggccggat gctgtttttg tattacgccg tagaaagaaa
      841 ggttgtcagt aaggggttac gttcacggga gatggcggct gccggaaact tcggcggtag
      901 cgtgcctgaa cgcaagcgta gcactgtact gtcacagccg ctttaacagc gaactgtctg
      961 tttttcctta accccggagg ttgctatgtc aaccggatta cgtttcacgc tggaagtgga
     1021 cggcctgcca ccggatgctt ttgcggtggt ctcctttcat ctgaaccagt cactctcttc
     1081 gcttttttcc ctcgatctct ctctggtcag ccagcagttt ctctcccttg aattccagca
     1141 gatcctcgac aaaatggcct acctgacgat atggcagggc gatgacgtac agcgccgggt
     1201 gaaaggtgtg gtgacctggt ttgaactggg ggagaacgac aaaaaccaga agctgtacag
     1261 catgaaggta tgcccgccgc tgtggcgcac agggctgcgc cagaacttcc gtatcttcca
     1321 gaatgaggac atcgcaagca tcctcggcac gatattgcag gaaaacgggg tgaccgagtg
     1381 gagcccgctg ttcagcgagc cacatccttc ccgtgagttt tgtgtccagt acggtgagac
     1441 tgattacgat ttcctgtgcc ggatggcggc ggaggaaggc atcttctttt atgaggagca
     1501 cgcgcaaaaa agtaccgacc agagcctggt cctgtgcgat accgtgcttt atctgccgga
     1561 gtcctttgag atcccctgga atccgaacac ccgtaccgag gtaagcaccc tctgcatcag
     1621 ccagtttcgc tacagcgcac aaatccgccc ttcttccgtg gtgaccaaag actacacctt
     1681 taaacgcccc ggctgggccg gacgttttga ccaggaaggc cagcaccagg attaccagcg
     1741 cacacagtat gaagtgtatg actatcccgg acgtttcaag ggcgcccacg ggcagaactt
     1801 tgcccgctgg cagatggacg gctggcgaaa caacgcagaa gtggcgcgcg gaacaagccg
     1861 ttcgccggag atatggccgg gacggcgaat tgtgctgacg gggcatccgc aggcgaacct
     1921 gaaccgggaa tggcaggtgg tggcaagtga actgcacggt gagcagccgc aggcggtgcc
     1981 gggacgcagg ggttcaggca ccacgctgga taaccatttt gcggtaatac cggcagacag
     2041 aacatggcga ccacagccgt tgctgaaacc gctggtggac ggcccgcaga gcgccgtcgt
     2101 gacgggaccg gcaggcgagg aaatcttctg tgatgaacat ggccgcgtgc gggtgaaatt
     2161 taactgggac cgctataacc cgtcaaacca ggacagttca tgctggatcc gtgtggcaca
     2221 ggcgtgggca ggcaccgggt ttggtaacct ggcgataccg cgtgtgggtc aggaggtcat
     2281 tgtggacttc ctcaacggcg atccggacca gccgatcatt atggggcgta cctaccacca
     2341 ggaaaaccgc acacccggta gcctgccggg gacgaagacg cagatgacca ttcgttcgaa
     2401 aacctataag ggcagcgggt ttaatgaact gaagtttgac gatgcgacag ggaaagaaca
     2461 ggtctacatc cacgcgcaga agaacatgaa caccgaggtg ctgaataacc gcaccacgga
     2521 tgtgataaac aaccatgccg aaaaaatagg taacaaccag gcgatcaccg ttaccaataa
     2581 ccagatcctg aacattggcg ttaatcagat acagacggtt ggtgtcaacc aggtggaaac
     2641 ggtaggcagt aaccagatta tcaaagtggg atcaaaccag gttgaaaagg tggggatcat
     2701 tcgtgcgctg acggtgggtg tggcgtatca gacgacggta ggcggcatta tgaatacctc
     2761 ggtggcgttg ttgcagtcct cacaggtaag gctgcataaa tcactgatgg tgggaatggg
     2821 ctacagcgtc aatgtaggga ataacgtcac cttctcggtg ggcaagacga tgaaggaaaa
     2881 caccggacaa acagcagttt attctgccgg tgagcatctt gaactctgct gtggtaaggc
     2941 aaggctggtg ctgacgaagg acggaagcat atttcttaat ggtacgcaca ttcatctgga
     3001 aggggagtcg gatgtgaacg gtgatgcgcc ggtgattaac tggaactgtg gtgccacaca
     3061 acctgtaccg gatgcgcctg tgccgaaaga tttacccccg gaatgccgga tatgcggcaa
     3121 ttttgagtca ggtgcaaaaa atcaaaacgg aactggtgca gtgaccagat aaaccatatt
     3181 aaaagcagaa ggaattgatt catgggagga aaaccggcgg cgcggcaggg tgacatgacc
     3241 cgcaagggac tggatattgt gcagggttca gcaggggtgc tgataggtgc gccgacgggc
     3301 gtggcctgct cggtgtgtcc gggagggatt acctatgcta acccggtgaa cccggtgctg
     3361 ggtgcgaagg tgctgccggg cgagacggac cttgcgctgc ccggcccgct gccgtttatt
     3421 ctttcccgcg cctacagcag ctaccggacc agaacacccg cgccggtggg ggtgtttggt
     3481 cccggctgga aagcgccctt cgatatccgc ttacagatac gcgatgaagg cctgatactc
     3541 aacgacaacg gcggcaggag cattcacttt gagccgctgt ttcccggcga gataagctac
     3601 agccgcagcg agtcgttctg gctggcgcgg ggcggggtgg cggagcagca cagttcgcag
     3661 ccgctaagcg cgctctggca ggtgctgccg gaagatgttc gcctgagtcc gcatatgtac
     3721 ctggcgacaa acagccttca ggggccgtgg tggatactca actggccgga gcgggtgccg
     3781 ggggcggacg aggtgctgcc gccggagccg cccgcatacc gggtgctgac gggcgtggtg
     3841 gatggcttcg ggcggacgct ggcctttcac cgggcggcgg agggtgatgt ggcgggcgcg
     3901 gtgacggggg tgacggacgg cgcgggccgc cgttttcacc tggtgctgac cacacaggca
     3961 cagcgggcgg aagtattccg taaacagcgc gccacgtctt tatcttcccc tgccggtccc
     4021 cgttctgctt cctcttctct ggttttcccc gacacgctgc ccgccggtac agaatacggt
     4081 gccgataacg gtatccggct ggaggcggta tggctgacac acgacccggc atacccggat
     4141 gaactgcccg ccgcgccgct ggcgcgctac acgtacacgg ccagcggaga actgcgggcg
     4201 gtgtatgacc gcagcgggac gcaggtgcgc gggtttgctt atgatgcgga gcacgccggg
     4261 cggatggtgg cgcaccatta tgcgggtagg ccggagagcc gctaccggta tgatgatacc
     4321 ggccgggtga cggagctggt caacccggag gggctggact accgctttga gtacgggcag
     4381 gaccgtgtga ccatcacgga cagcctgaac cggcgggagg tgctgtacac ggaaggcgag
     4441 ggtggcctga aacgtgttgt gaagaaggaa catgcggacg ggagcatcac ccgcagcgag
     4501 tatgatgagg cggggaggct gaaggcacag acggatgcgg cgggacggcg gacggagtac
     4561 agcctgcata tggcgtcggg tgcggtgaca gcggtgacgg ggccggacgg caggacggtg
     4621 cggtatggct ataacagcca gcggcaggtg acgtcagtga cgtacccgga cgggctgcgc
     4681 agcagccggg agtatgatga gaagggaagg ctggcggcgg agacctcgcg cagcggagag
     4741 acgacgcggt acagctatga tgacccggcg agtgagctgc cgacagggat acaggacgcg
     4801 acgggcagta caaaacagat ggcatggagc cgttacggtc agctgctgac ctttacggac
     4861 tgctcggggt acacgacgcg gtatgagtat gaccggtacg gtcaacaaat cgccgttcac
     4921 cgggaagaag gcatcagcac ttacagcagt tataacccgc gtggccaact ggtcagtcag
     4981 aaggatgcgc aggggcgtga aacccgttat gagtacagcg ccgcaggcga cctcaccgct
     5041 atcgttgccc cggacggcag ccgcagtgag atacagtatg atgcgtgggg aaaggccgtc
     5101 agcaccacgc agggcggtct gacgcgcagc atggggtatg acgctgccgg gcgcatcacc
     5161 gtgctgacca acgagaacgg cagccagtcc acgttccggt atgacccggt ggacaggctg
     5221 actgaacagc gcggttttga cggccggacg caacgttacc actatgacct gaccggaaaa
     5281 ctcacgcaga gtgaagacga ggggcttatc accctctggc actacgatgc gtcggaccgc
     5341 atcacgcacc ggacggtgaa cggcgacccg gcagagcagt ggcagtatga tgagcacggg
     5401 tggctaacca ccctcagcca taccagtgaa ggccaccggg tgtcggtcca ctacggctat
     5461 gacgataaag gccgcctgac gggtgaacgg cagacggtgg agaacccgga gacgggggag
     5521 atgctgtggg agcatgagac ggggcacgcg tacagcgaac aggggctggc gacccgtcag
     5581 gagccggacg gtctgccgcc ggtagagtgg ctgacgtatg gcagcggtta tcttgcgggg
     5641 atgaagctgg gcggaacgcc actggtcgag tacatgcggg accggctgca ccgtgagacg
     5701 gcccgcagct tcggcgggga ggcatatgaa cttgccaccg cctggaatac cagcggccag
     5761 ctccggagca ggcacctgaa ccttccgcag cttgaccgtg actacgactg gaacgacaac
     5821 ggacagctaa tccgcatcag cggcccgcag gagagccggg agtaccgtta cagtgacacg
     5881 ggaaggctga cgggcgtcca caccacggca gcgaacctgg atatcgatat cccgtatgca
     5941 acggacccgg caggaaaccg gctgccggac ccggaactgc atccggacag cacgctcacg
     6001 gcgtggccgg ataaccgcat cgcggaagat gcgcactata tctatcgcta cgatgaatac
     6061 ggcaggctgg cggagaagac ggaccg
//
LOCUS       DAZKIP010000198         6071 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZKIP010000198 DAZKIP010000000
VERSION     DAZKIP010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.200"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..253
                     /locus_tag="ACHOUK_004527"
     CDS             <1..253
                     /locus_tag="ACHOUK_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFP9899824.1"
                     /translation="SGAVIRGVFDDPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRG
                     DTLTIGEENFWIDRISTDDGGSCHLWLGRGVPPAVNRRR"
     gene            265..842
                     /locus_tag="ACHOUK_004528"
                     /pseudo
     CDS             265..842
                     /locus_tag="ACHOUK_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            839..1234
                     /locus_tag="ACHOUK_004529"
     CDS             839..1234
                     /locus_tag="ACHOUK_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFP9899825.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVLDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            1242..1982
                     /locus_tag="ACHOUK_004530"
     CDS             1242..1982
                     /locus_tag="ACHOUK_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9899826.1"
                     /translation="MPTPNPLAPVKGAGTTLWVYNGNGDPYANPLSDNDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWAATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEINVTAS"
     gene            1998..2420
                     /locus_tag="ACHOUK_004531"
     CDS             1998..2420
                     /locus_tag="ACHOUK_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFP9899827.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPKKTQMPSMNEAVKQIEQEVLTTWPAEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            2402..2836
                     /locus_tag="ACHOUK_004532"
     CDS             2402..2836
                     /locus_tag="ACHOUK_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFP9899828.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            2829..5378
                     /locus_tag="ACHOUK_004533"
     CDS             2829..5378
                     /locus_tag="ACHOUK_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9899829.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSMSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGEEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQSQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRMLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAVLGDKVTYQEHLNALAQQADKFA
                     QQQRAKRAAIDAKNRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLRSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGGASASGGTAIQAAAAKFHFAAGGFTGTGGK
                     YEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMADSRSQASGTFE
                     QNNHVVINNDGTNGQIGPQALKAVYDVARKAAMDVVTGQMRDGGLFSGGGR"
     gene            5375..5704
                     /locus_tag="ACHOUK_004534"
     CDS             5375..5704
                     /locus_tag="ACHOUK_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9899830.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNADLKTY
                     SVTLSVPRWEATALESFLAEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            5704..>6071
                     /locus_tag="ACHOUK_004535"
     CDS             5704..>6071
                     /locus_tag="ACHOUK_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP9899831.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGN"
BASE COUNT         1409 a         1487 c         1988 g         1187 t
ORIGIN      
        1 gtccggcgca gtaatacgtg gtgtttttga tgaccctgaa aatatcagct atgccggaca
       61 gggcgtgcgt gttgaaggct ccagcccgtc cctgtttgtc cggactgatg atgtgcggca
      121 actgcggcgt ggagacacgc tgaccatcgg tgaggaaaac ttctggatag accggatttc
      181 gacggatgat ggcggaagct gtcatctctg gcttgggcgg ggcgtaccgc ctgccgttaa
      241 ccgtcgtcgc tgaagggggg atgtatggcc ataaaaggtc ttgagcaggc cgttgaaaac
      301 ctcagtcgta tcagcaaaac ggcggtgcct ggtgcgtcag caatggccat taaccgcgtt
      361 gcttcatccg cgatatcgca gtcggcgtca caggttgccc gtgagacaag ggtacgccgg
      421 aaactggtaa aggaaagggc caggctgaaa agggccacgg ttaaaaatcc gcaggcagaa
      481 tcaaggttaa ccggggggat ttgcccgtaa tcaagctggg taacgcgcgg gttgtcctgt
      541 cccgacgcag gcgtcgtaaa aaggggcagc gttcatccct gaaaggtggc ggcagcgtgc
      601 ttgtggtggg aaaccgtcgt attcccggcg cgtttattca gcaactgaaa aatggccggt
      661 ggcatgtcat gcagcgtgtg gccgggaaaa accgttaccc cattgatgtg gtgaaaatcc
      721 cgatggcggt gccgctgacc acggcgttta aacagaatat tgagcggata cggcgtgaac
      781 gtcttccgaa agagctgggc tatgcgctgc agcatcaact gagaatggta ataaagcgat
      841 gaaacatact gaactccgtg cagccgtact ggatgcactg gagaagcatg acaccggggc
      901 gacgtttttt gatggtcgcc ccgctgtttt ggatgaggcg gattttccgg cagttgccgt
      961 ttatctcacc ggcgctgaat acacgggcga agagctggac agcgatacct ggcaggcgga
     1021 gctgcatatc gaagttttcc tgcctgctca ggtgccggat tcagagctgg atgcgtggat
     1081 ggagtcccgg atttatccgg tgatgagcga tatcccggca ctatcagatt tgatcaccag
     1141 tatggtggcc agtggctatg actaccggcg cgacgatgat gcgggcctgt ggagttcagc
     1201 cgatctgact tatgtcatta cctatgaaat gtgaggacga tatgccaaca ccaaatcctc
     1261 tggcaccggt gaaaggggcc gggaccacac tgtgggttta taacgggaac ggcgacccat
     1321 atgcaaaccc gctttcagac aatgactggt cgcgtctggc aaaggttaaa gacctgacgc
     1381 ccggcgaact gaccgctgag tcctatgacg acagttatct cgatgatgaa gatgcggact
     1441 gggccgcgac cggacagggg cagaaatccg ctggagatac cagcttcacg ctggcgtgga
     1501 tgcccggaga gcaggggcag caggcgctgc tggcgtggtt taatgaaggt gatacccgtg
     1561 cctataaaat ccgcttcccg aacggcacgg tcgatgtgtt ccgcggctgg gtcagcagta
     1621 tcggtaaggc ggtgacggcg aaggaagtga tcacccgcac ggtgaaagtc accaacgtgg
     1681 gacgtccgtc gatggcagaa gatcgcagca cggtaacagc ggcaaccggc atgaccgtga
     1741 cgcctgccag cacctcggtg gtgaaagggc agagcacgac gctgaccgtg gcattccagc
     1801 cggaaggcgc aaccgacaag agcttccgtg cggtgtctgc ggataaaaca aaagccaccg
     1861 tgtcggtcag tggtatgacc atcaccgtga aaggtgttgc tgcaggcaag gtcaacattc
     1921 cggtcgtatc cggtaatggt gagtttgctg cggttgcaga aatcaacgtc accgccagtt
     1981 aatccggaga atcagcgatg ttcctgaaaa ccgaatcatt tgaacataac ggcgtgaccg
     2041 tcacgctttc tgaactgtca gccctgcagc gtattgagca tctcgccctg atgaaacggc
     2101 aggcagaaca ggcggagtca gacagcaacc ggaagtttac tgtggaagac gccatcagaa
     2161 ccggcgcttt tgtggtggcg atgtccctgt ggcataacca tccgaagaag acgcagatgc
     2221 cgtccatgaa tgaagccgtt aaacagattg agcaggaagt gcttaccacc tggcccgcgg
     2281 aggcaatttc tcatgctgaa aacgtggtgt accggctgtc cggtatgtat gagtttgtgg
     2341 tgaatgatgc ccctgaacag gcagaggacg ccgggcctgc agagcctgtt tctgcgggaa
     2401 agtgttcgac ggtgagctga gttttgccct gaaactggcg cgcgagatgg ggcgacccga
     2461 ctggcgtgcc atgcttgccg ggatgtcatc cacggagtat gccgactggc accgctttta
     2521 cagtacccat tattttcatg atgttctgct ggatatgcac ttttccgggc tgacgtacac
     2581 cgtgctcagc ctgtttttca gcgatccgga tatgcatccg ctggatttca gtctgctgaa
     2641 ccggcgtgag gctgacgaag agcctgaaga tgatgtgctg atgcagaaag cggcagggct
     2701 tgctggaggc gttcgttttg gcccggacgg gaatgaagtt atccccgctt ccccggatgt
     2761 ggcggacatg acggaggatg acgtaatgct gatgacagta tcagaaggga tcgcaggagg
     2821 agtccggtat ggctgaaccg gtaggcgatc tggtcgttga tttaagtctg gatgcggcca
     2881 gatttgacga gcagatggcc agagtcaggc gtcatttttc cggtacggaa agtgatgcga
     2941 aaaaaacagc ggcagtcgtt gaacagtcga tgagccggca ggcgctggct gcacagaaag
     3001 cgggaatttc cgtcgggcag tataaagccg ccatgcgtat gctgcctgcg cagttcactg
     3061 acgtggccac gcagcttgca ggtgggcaaa gtccgtggct gatcctgctg caacaggggg
     3121 ggcaggtgaa ggactccttc ggcgggatga tccccatgtt ccgggggctt gccggtgcga
     3181 tcaccctgcc gatggtgggg gccacctcgc tggcggtggc gaccggtgcg ctggcgtatg
     3241 cctggtatca gggcaactca accctgtccg atttcaacaa aacgctggtc ctttccggca
     3301 atcaggccgg gctgacggca gatcgtatgc tggtcctgtc cagagccggg caggcggcag
     3361 ggctgacgtt taaccagacc agcgagtcac tcagcgcact ggttaaggcg ggggtaagcg
     3421 gtgaggctca gattgcgtcc atcagccaga gtgtggcgcg tttctcctct gcatcaggcg
     3481 tggaggtgga caaggtcgct gaagccttcg ggaagctgac cactgacccg acgtcggggc
     3541 tgacagcgat ggcgcgccag ttccataacg tgacggcgga gcagattgcg tatgttgctc
     3601 agttgcagcg ttccggcgaa gaagccgggg cattgcaggc ggcgaacgag gccgcgacga
     3661 aagggtttga tgaccagacc cgccgcctga aagagaacat gggcacgctg gaaacctggg
     3721 cagacaggac agcacgggca ttcaaatcca tgtgggatgc ggtgctggat attggtcgtc
     3781 ctgataccgc gcaggagatg ctgattaagg cagaggccgc gtttaagaaa gcggacgata
     3841 tctggaatct gcgcaaggat gattattttg ttaacgatga agcgcgggcg cgttactggg
     3901 atgatcgtga aaaggcccgt cttgcgcttg aagccgcccg aaagaaggct gagcagcaga
     3961 gtcaacagga caaaaatgcg cagcaacaga gcgataccga agcgtcacgg ctgaaatata
     4021 ccgaagaggc gcagaaggct tacgaacgcc tgcagacgcc gctggagaaa tataccgccc
     4081 gtcaggaaga actgaataag gcactgaaag acgggaaaat cctgcaggca gattacaaca
     4141 cgctgatggc ggcggcgaaa aaggactatg aagcgacgct gaaaaagccg aaacagtccg
     4201 gcgtgaaggt gtctgcgggc gatcgtcagg aagacagtgc tcatgctgcc ctgctgacgc
     4261 ttcaggcaga actccggatg ctggagaagc atgccggagc gaatgagaaa atcagccagc
     4321 agcgccggga tttgtggaag gcagaaagtc agttcgcggt actggaggag gcggcacaac
     4381 gtcgccagct gtccgcacag gagaaatccc tgctggcgca taaagacgag acgctggagt
     4441 acaaacgcca gctggctgta cttggcgaca aggtcaccta tcaggagcac ctgaacgcgc
     4501 tggcacagca ggcggataaa ttcgcacagc aacaacgggc aaaacgggcc gccattgatg
     4561 caaaaaaccg ggggctgact gaccggcagg cagcgcggga agccacggaa cagcgcctga
     4621 aggaacagta tggcgataat cctctggcgc tgaataacgt catgtcagag cagaaaaaga
     4681 cctgggcagc tgaagaccag cttcgcggga gctggatggc aggcctcagg tcaggctgga
     4741 gtgagtggga agagagcgcc acggacagta tgtcgcaggt taaaagtgct gccacgcaga
     4801 cctttgatgg tattgcacag aatatggcgg cgatgctgac cggcagtgaa cagaactggc
     4861 gcagcttcac ccgttccgtg ctgtccatga tgacagaaat tctgcttaag caggcaatgg
     4921 tggggattgt cggaagtata ggcagcgcca ttggcggcgg cgcatcagcg tcaggcggta
     4981 cagccattca ggcagctgcg gcgaaattcc attttgcggc cggagggttt acgggaaccg
     5041 gcggcaaata tgagccagcg gggattgttc accgtggtga atttgtcttc acgaaggagg
     5101 caaccagccg gattggcgta ggaaatcttt accggctgat gcgcggctat gccaccggcg
     5161 gttatgtcgg tacaccgggc agcatggcgg acagccggtc gcaggcgtcc gggacgtttg
     5221 agcagaataa ccatgtggtg attaacaacg acggcacgaa cggtcagata gggccacagg
     5281 cactgaaggc ggtttatgac gtagcccgta aggcggcaat ggatgttgtg accgggcaga
     5341 tgcgcgatgg tggtctgttc tccggaggtg gacgatgaaa accttccgct ggaaagtgaa
     5401 acccggtatg gatgtggctt cggccccttc cgtaagaaag gtgcgctttg gtgatggcta
     5461 ttcccagcga gcgcctgccg ggctgaatgc cgacctgaaa acgtacagcg tgacgctttc
     5521 tgttccccgt tgggaggcca cggcgcttga gtcgtttctg gctgagcacg ggggctggaa
     5581 ggcctttctg tggacgccgc cttatgagtg gcggcagata aaggtgacct gcgcaaaatg
     5641 gtcgtcgcgg gtcagtatgc tgcgtgttga gttcagcgca gagtttgaac aggtggtgaa
     5701 ctgatgcagg atatccggca ggaaacactg aatgaatgca cccgtgcgga gcagtcggcc
     5761 agcgtggtgc tctgggaaat cgatctgaca gaggtcggtg gagaacgtta ttttttctgt
     5821 aatgagcaga acgaaaaagg tgagccggtc acctggcagg ggcgacagta tcagccgtat
     5881 cccattcagg ggagtggttt tgaactgaat ggcaaaggca ccagtacgcg ccccacgctg
     5941 acggtttcta acctgtacgg tatggtcacc gggatggcgg aagatctgca gagtctggtc
     6001 ggcggaacgg tggtccggcg taaggtttac gcccgttttc tggatgcggt gaacttcgtc
     6061 aacggaaaca g
//
LOCUS       DAZKIP010000199         6049 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZKIP010000199 DAZKIP010000000
VERSION     DAZKIP010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.201"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..2390)
                     /locus_tag="ACHOUK_004536"
     CDS             complement(<1..2390)
                     /locus_tag="ACHOUK_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9899832.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELHSQLTEIRASAVGMTGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFASSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTG
                     LKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIVRALAAGMGVARKDLK
                     AMADDGKLTADKVVPALISQLGILRDEYAAMPETVSSSITKVENAFMAWVGGANEASG
                     VTKTLSGMLNGVAGQIDNVATAVGALVAVGVARYFGNMASGAMSATAGLVTAARNEVA
                     LAEAQFRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNRNIAARS
                     AAQNALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALT
                     IDEIAHKTPSMSLPEASDNEGRTRAALTEQNRLIDEQASRVKSLQEKIAGYQYVLANP
                     GWTTGDGFMINHLTSVKTVTEGLSQATEQLAVEQSRLAQMQEKAQSIQDVLAGLEDRR
                     VALIRQQAAEQNKVYQSMLVMNGQHTEFNRLLGLGNELLQQRQGLVNVPLRLPQATLD
                     DKQQSALTKTERELALSRLKGEEKERVRLGYAADDLGFVGDPYQEARQRYISNALEAW
                     RNNEANKPKSRGGKSETEKAEDSFSRLLKQQKEQLALVGQNTELAKLKYQTALGELKT
                     LTEMQKQELLRNATLIDQQKIREQLRSREETLKNENAAARASNDAELLGYGQGERA"
     gene            complement(2438..2647)
                     /locus_tag="ACHOUK_004537"
     CDS             complement(2438..2647)
                     /locus_tag="ACHOUK_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFP9899833.1"
                     /translation="MWMEFDRISPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEVDPFAALEDALSLAAQS"
     gene            complement(2743..3117)
                     /locus_tag="ACHOUK_004538"
     CDS             complement(2743..3117)
                     /locus_tag="ACHOUK_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFP9899834.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            complement(3132..3848)
                     /locus_tag="ACHOUK_004539"
     CDS             complement(3132..3848)
                     /locus_tag="ACHOUK_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFP9899835.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVL
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSFVVPLAFVKNLDKTLTVN
                     TGALLTMSVSANGGTPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTCVVTDSA
                     EKAQSVTSVECTVTVSAAAG"
     gene            complement(3914..4258)
                     /locus_tag="ACHOUK_004540"
     CDS             complement(3914..4258)
                     /locus_tag="ACHOUK_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFP9899836.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVSVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            complement(4255..4701)
                     /locus_tag="ACHOUK_004541"
     CDS             complement(4255..4701)
                     /locus_tag="ACHOUK_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFP9899837.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            complement(4698..5048)
                     /locus_tag="ACHOUK_004542"
     CDS             complement(4698..5048)
                     /locus_tag="ACHOUK_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFP9899838.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GGRLEILCRLGGEK"
     gene            complement(5058..5384)
                     /locus_tag="ACHOUK_004543"
     CDS             complement(5058..5384)
                     /locus_tag="ACHOUK_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFP9899839.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELIQGELPFSVSVLIYDL
                     RCPTVL"
     gene            complement(5387..>6049)
                     /locus_tag="ACHOUK_004544"
     CDS             complement(5387..>6049)
                     /locus_tag="ACHOUK_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9899840.1"
                     /translation="DGRDYDPEVLQKAVNDAVANIPQPADGKSLTPDDVRPMLEQMVK
                     EAVSHIPVPRDGRDYDPDVLQKAVLDAVSALPAPQDGRDATALEILPAIDDQKSFPRG
                     TYATHQGGLWRAYEKTHGMRGWECLVDGVADIDVSMTGERLFSVVVRQSSGQRTEKTF
                     SLPVMLYRGVFRAGETYHPGDTVTWGGSLWHCNSMTEDKPGEAHSSAWTLAAKRGRDA
                     GG"
BASE COUNT         1217 a         2027 c         1447 g         1358 t
ORIGIN      
        1 ctggctcgct ccccctgccc gtaccccagc agttcagcat cattcgacgc acgcgcagcc
       61 gcattctcat tcttcagtgt ctcttcccgg gatcgcaact gttcccggat tttttgctgg
      121 tcaatcaggg tcgcgttacg cagcagttcc tgcttctgca tctccgtcag ggttttcagt
      181 tcacccagcg ctgtctggta tttcagcttc gccagctctg tattctgccc caccagtgcc
      241 agttgctctt tctgctgctt cagcagccgg gaaaaactgt cttccgcttt ttccgtctct
      301 gattttccac cccgggattt gggtttattc gcctcgttat tgcgccaggc ttccagggca
      361 ttactgatat aacgttgtct cgcctcctga tacggatcac ccacaaaacc gaggtcatcc
      421 gccgcatacc ccagtcggac acgctctttt tcttcccctt tcagtctgga cagggccagc
      481 tcacgctctg tttttgtcag ggcactctgc tgtttatcat ccagggtggc ctgcggcagc
      541 cgtaacggta cattcaccag tccctgacgc tgctgaagca gttcattccc cagccccagc
      601 agacggttga attccgtatg ctgaccgttc ataaccagca tggactggta caccttattc
      661 tgctctgccg cctgctgacg aattaacgcc acacgacggt cttccagccc ggcaagcaca
      721 tcctgaatgg actgcgcttt ttcctgcatc tgtgccagac gggactgctc aacggcaagc
      781 tgctctgttg cctgagaaag cccttccgtt acggtcttca ccgatgtcag atggtttatc
      841 atgaatccgt caccggtcgt ccagcccggg ttcgccagaa catactgata tcctgcgatt
      901 ttttcctgca gggatttcac ccgactggcc tgttcatcaa tcagccggtt ctgctctgtc
      961 agcgccgccc gtgttcgtcc ttcattatct gaggcttcag gcaaagacat tgacggcgtt
     1021 ttatgcgcga tttcatctat cgtcagtgca tactggcgcg cagactccct ggcctgctcc
     1081 tgattctggt acagcgtgta ccatgctgca gcccccagca tcaccagtcc gggtacgcca
     1141 ccaaccagcc ccagcgcacc gctcatcaga cgtgagccca ccgccgttgt actgttcagc
     1201 gcattctggg cggcgcttct ggcagcaata tttctgttca ggcgttcctg tgtggccgcc
     1261 agacgggcct ctgcagcaat ctgcatctcc gtcccgcggg ctgccgccac ggcctgctga
     1321 gcacggtaca cggctgccct tgcccgcgcc gtggcaatct gcgttcccct gaactgtgct
     1381 tccgccagtg caacttcatt acgtgcagcc gtcacaagtc ctgccgtggc agacatcgct
     1441 ccggaggcca tattgccaaa gtaccgggca accccgacgg caaccagcgc gcccacggct
     1501 gttgccacat tatcaatctg tccggcaaca ccgttcagca tgccggagag cgtttttgtc
     1561 accccgctgg cctcattcgc accgcccacc caggccataa aggcgttttc cacctttgtg
     1621 atactactgg aaaccgtttc cggcatggcc gcatattcat cacgtaatat ccccagctgg
     1681 ctgattaacg cggggaccac tttatccgct gtcagttttc cgtcatccgc cattgccttc
     1741 agatctttac gggccacgcc catacccgca gccagtgcac gtacgatccg gtcaccactt
     1801 tcattgaccg aattaaactc ctcaccacgc aatacaccct gcgccagcgc ctgactgaac
     1861 tgggtgatca ccgagcccgc ctctgccgta ctggcaccgg agattttcag ccctgtcgaa
     1921 atggcctccg tcaccttcag cacatcatca gcactgtaac catattcacg catcgaggca
     1981 gccgaacggg caaacagggc cgcattatcc gaaaatgcgg tgcctgtccg ctggctgata
     2041 tccatcagca ctttctgtga tgacgcaaat tcatccgatg actgcgacgc ctgtttcaga
     2101 cgggcattca cggagctcca ttcatccgcc agcgaaatca ggtgtccggt ggcaaaggca
     2161 ccggcaaacg caccggtcat tccgacagcc gaagcgcgga tttccgtcaa ctggctgtgc
     2221 agctcagcca gagcccggcg ctgctccctg gctgccgcag cagcctgacg tccgccattc
     2281 tgcagggtcc ggtaatattc actgcccata cgggacgccc gctggatctc cgactggaat
     2341 gactgtgaat ttgccgaaat tttgataatc agttcacgta acgtcgccat tcacctttct
     2401 ccgggcgtaa aaaaaccgcc tcagcggttc tcatcattca tgactgtgct gcaaggctca
     2461 gcgcgtcttc cagcgccgca aacggatcca cctccggctt atcctcatcc tcgccccagc
     2521 agagcatggc gtccttcagt gcaacattca tcccctgtgc cccgaaaacc gctttcacga
     2581 tctgtgcatt acggatatcc ccgcgctcat cacccagcgg ggatatcctg tcgaactcca
     2641 tccacatcat cgcctcgctc gcactcaggc tgtggcgcag ttcggataag gtgcgcccca
     2701 gacggagcgc aagtcgcatc agaaagcgaa tttccgggcg ggctactttt ttctggccga
     2761 ctctgcatca gcgatcagtt ccagtgcctg acgcagcaac cgggcatgta ccggaccata
     2821 gacggccagc acctgctcac ggtcgtccgg agtgaacacc cgctgcaggt ccgtatcaca
     2881 caggacatcg cagaacagcg tcacatccgc ttccaggtta cggcgggttt tcgccaccac
     2941 cgaaagggta tcgtcatcct ctccatcacc attgagcact tcctgccaca gataccaggc
     3001 ctctgccgaa ggctcccgca gcaccacgct gacattaccc cattccggca ccttcaccgt
     3061 tttatgacga aaccctgaca gtctggccag cgccagcgtt ttcagatcct ttttcatgat
     3121 gacccatccc cttatccggc ggctgcgctc actgtcacgg tgcattcaac agacgtcaca
     3181 ctctgtgctt tctctgccga atcggtcacc acacaggtat atttccccgc atcagcggac
     3241 tgcgcacctg gcttactgaa ggtgtctgtc gtctgcccgt caaccggctg accatccttc
     3301 ttccaggcgt atttatacgg cggcgttccc ccgttggcac tgactgacat tgtcagcagc
     3361 gcaccggtat tcacggtaag tgtcttatcc agatttttca caaacgccag cggtaccaca
     3421 aaggacaccg gtttgccttt cagacgcagt gagaacgttg cagccaccac gccgttggta
     3481 ccggatgacc aggtgtgctg gcgcacttcc gccaggaact taaagccctt accggacgga
     3541 aacagcacct taaacgcata caacgcgtca ttgtcatagg catcacgcag ggcgttctgg
     3601 gcctgattca gataaaaatt gcccgacatg gaaatctcag acgacgcccc cagaccgttg
     3661 atgttctcct gctctgtgga gcagagcgtg gtcacatcaa tatcctgttt ctgcccggcg
     3721 gtgaactgca cttccttgat ggtgcagtcc aggcgcagat attccgcctt atccatagtt
     3781 tcagcagtcg ccggggcaga tgaaatcatc acctgcgtca gctgtgagcg ttcatacaaa
     3841 gcagacattc tgcctcctga taataaaaaa cccgcacgcg gcggggtatg ggttttgtag
     3901 aaaaaagaaa aagtcacacc gtgacctgaa actccagggt tgcacggtaa cagcggtttt
     3961 ccggaatata gtcctgcatt tcactgacgg atcccggggc cagcagcatt atggcttcac
     4021 gggcgtcctg acgtatctga cgcgcctgcg tcacagtccc ggcataaacg tctatctgca
     4081 ccgacactga ggactccgcc tgcccgccca tcacgtccgc tgacaccgat gaaatcaggc
     4141 tgaaaaccac ccacggaagc gccaccgacg gcctgccatc cagcaggggg accacatacg
     4201 ggtacacctg cccgccggca agatgcgcca gatgaggata caaatccgcc tccgtcatcg
     4261 tctcagtacc tcatcaatgg cccggttcat ccgcgcaatc gccacctgtg ccgcctgttc
     4321 actgcgcaca tcaaatgccg ggcgcacaaa cgggtgcggt ggcatattca cggtccccat
     4381 ttccacaaac cgccagtaga aagcgttgcg cgggttatcc gccttcatgg tgttatcgct
     4441 gttaccggtg tccggattaa caccccggat atgcacaccg gattccatcc cgccatcgcg
     4501 ggagcgccgg gaaaggacca ccacattgcg gcgcagtttt cccctgcgta ccggtgcccg
     4561 tgacaccact tcttctttca gcacattcgc acccgcacgg gttgcctcac gcagcacccg
     4621 gttattttct gcaccactca gaagctgcaa atcgcggctg atgtcctcca gccccgaaaa
     4681 atccagcagg gtttcgatca tttttcccct cccagccgac agagaatttc cagacgtccg
     4741 ccggtcgcat ccggcacggg cagcccgaca acgttcagga tccggtcacg ccagggacca
     4801 ctcagcacat gaagtcgtga cgctgccgtg atttcccgac cggactgacc gcgcacccag
     4861 atgcggattt ccgcctgcgc catttccgca ccggactgca tccgctcccg gctgctcctg
     4921 ccacggatat ccgcatgaat tttcccgcat gacacccatt cttccgtcat ttctccggca
     4981 gcattacggg ttaacaccgg gttcagaaca cttatcatct gtgtcagacg acctgcagat
     5041 attgccattc cccctcctca taacaccgtc ggacaacgca aatcgtaaat cagcacggaa
     5101 acagaaaacg gcagctcccc ctgaatcagt tcttcccgct ccgcaagatc cggattccgg
     5161 tacagcatcc cggtcagtcg catggcagcc cccttcatcc gggttaatgc ctcgcccggg
     5221 atcagttcac cgtcctcacg aatcacctta tcccggctgc cctgaatgta ggccagcagc
     5281 acggcggtag cctgacgaac cttgtccatc agcatgtcat catccgcgtc atggtcgaca
     5341 cgcagatgtg ccttgatctc ttccagtgtc agtaatgccg tcattttcag cctcctgcat
     5401 cccgcccacg ttttgcagcc agggtccagg ctgatgaatg agcttctccg ggtttatctt
     5461 cggtcatact gttgcagtgc cacagcgagc ccccccacgt caccgtatcg ccggggtggt
     5521 aggtttcacc ggctctgaac acaccgcggt agagcatcac cggcagggaa aatgtttttt
     5581 ccgtacgctg gccactgctc tgccggacca ccacagagaa caaccgctca cccgtcatgc
     5641 tgacgtcaat atccgccacc ccgtcaacca ggcattccca tccccgcatc ccgtgcgttt
     5701 tttcatacgc ccgccagagt ccgccctggt gtgtggcata cgtgccccgg ggaaaggatt
     5761 tttgatcgtc aatggcgggg agtatttcca gagccgtggc atcacgcccg tcctgcggag
     5821 ccggcagggc actcaccgca tccagaaccg ccttctgcag aacatccgga tcgtagtcac
     5881 gaccatcacg cggaacagga atatggctta ccgcctcctt caccatctgt tcaagcatcg
     5941 gacgcacatc atccggggtg agacttttac cgtctgccgg ctgcggaata tttgcgaccg
     6001 catcattcac cgccttctgc agtacttccg gatcatagtc acgaccgtc
//
LOCUS       DAZKIP010000200         6036 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZKIP010000200 DAZKIP010000000
VERSION     DAZKIP010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.202"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            129..857
                     /gene="gadW"
                     /locus_tag="ACHOUK_004545"
     CDS             129..857
                     /gene="gadW"
                     /locus_tag="ACHOUK_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFP9899841.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(1220..4333)
                     /gene="mdtF"
                     /locus_tag="ACHOUK_004546"
     CDS             complement(1220..4333)
                     /gene="mdtF"
                     /locus_tag="ACHOUK_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFP9899842.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVIYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(4358..5467)
                     /gene="mdtE"
                     /locus_tag="ACHOUK_004547"
     CDS             complement(4358..5467)
                     /gene="mdtE"
                     /locus_tag="ACHOUK_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFP9899843.1"
                     /translation="MLTACDDKSAENTAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVKLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            5575..5853
                     /locus_tag="ACHOUK_004548"
     CDS             5575..5853
                     /locus_tag="ACHOUK_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899844.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(5854..>6036)
                     /gene="gadE"
                     /locus_tag="ACHOUK_004549"
     CDS             complement(5854..>6036)
                     /gene="gadE"
                     /locus_tag="ACHOUK_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFP9899845.1"
                     /translation="FSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLK
                     LRRMSDIVTLGITSYF"
BASE COUNT         1485 a         1500 c         1516 g         1535 t
ORIGIN      
        1 aaaactactt tgtggataaa ttttggtcct accaaatttg gcagtttttg cgctaagaaa
       61 cagtctggca tcatttcatt agtatactga aattgaaata atcgcagtat gaaatataag
      121 ggataatcat gactcatgtc tgctcggtga tcctcattcg tcgttcattc gatatttatc
      181 atgaacagca aaaaatatcg ctgcataacg agagtatcct gctgctggag aaaaatttgg
      241 cagacgattt tgcgttttgt tcaccggata cgcgacgact ggatatcgat gagctgacag
      301 tttgccatta cttacaaaat attcgtcagc taccacgcaa tttagggtta catagcaaag
      361 accgtctgtt aattaaccag tcacccccca tgccgctggt gacggcgatt tttgatagct
      421 tcaatgaatc cggggtaaat tcaccgatac tgagcaatat gctctacctt tcctgtttat
      481 cgatgttttc tcataagaaa gaactgatcc ccttactttt caatagcatc agcactgttt
      541 caggaaaagt tgaacgcctt attagctttg atatcgccaa acgttggtat ctgcgcgata
      601 tcgcggaaag aatgtatacc agcgagagtc tcatcaaaaa aaagttgcag gatgaaaaca
      661 cctgtttcag taaaatatta ctcgcctcca ggatgtcgat ggccagacga ttactcgagt
      721 tacgtcaaat tcctctgcat actattgcgg aaaaatgtgg ctatagcagt acgtcgtact
      781 ttataaacac atttcgacaa tattatggtg taacgccaca tcagtttgcg caacattcgc
      841 caggtacctt ttcctgacat attttgcatt tgaatattgg tcaggatctc acacctgctt
      901 catgtgaaac tcttccctga tgatttctgc cgggctaccg gctagttctc tttcgcagtt
      961 tgccaggcgg tataggtatt ttcaggagga aaagactcgg catgtttggg attattaagc
     1021 tgacaattca tacgattacg ggaatgtggg tcagtattgt gctgttcaaa ttgatgacga
     1081 atggctggag tggtttctac tttcagtgct gtgtactaag cctggtattc ctgacggtta
     1141 gctggttgtt gagtggtgaa tggttagcag gaaagagtaa ggctgaacct tcacgttcaa
     1201 ccttactctc atttacacgt tacgcttttt taaagcgggc aaagagatgt tccactacaa
     1261 cgaaaaagac cggaacaaag taaattgcca gcactgttgc ggcaaacatc ccgcccatca
     1321 cgccggtacc taccgcgttt tgcgcgccag aaccggcacc atgactgata accagcggca
     1381 gcacgccgag aataaaggcc agagaggtca tcaggattgg gcgtaaacgc atccgcgccg
     1441 cttcgatgat tgcctctatc ggcgttttcc cttctttctg catcatctca acggcaaatt
     1501 cgacaatcag gatggcgttc ttggcggaaa gcccgatggt ggtcagcaaa ccaacctgga
     1561 agtagacgtc attacttaag ccgcgcagat cggtggccag taatgcgcca acgacgccta
     1621 acggaacaac caacatcacc gagaacggaa ttgaccagct ctcataaagt gcggcgaggg
     1681 cgaggaacac cacgaccagt gaaatcgcat acagcgcagg agcctgattt gaggataacg
     1741 cttcctgata cgacagtccg gtccatgagt agccgacgcc tgccggaagt ttagcgacca
     1801 ggtctgccat aaatttcatg gcgtcaccgg tacttttccc ggccgccgct tcacctaaaa
     1861 tctccattga cgggatgccg ttgtagcgtt ccagtcgcgg tgaaccatag gtccattcgg
     1921 tagacgagta ggcagaaagc ggtgccatcg tgccagaggc gttgcgtaca taccattggt
     1981 tgatgttatc cggcaacata cggaacggcg tgcctgcctg gacatacact tttttcaccc
     2041 gcccctggtt gaggaagtcg ttcacgtagc tgctgccgaa ggcggtggaa attgtctggt
     2101 tgatatcaga cagcgccaca cccattgcct cagctttcgc ggcgttgacg ttcactttga
     2161 acatcggcgt atcttccagg ccgttcgggc gtaccccggt gacctgattc ggtgattgcg
     2221 ctgccagtga taacagctcg tttcgtgcct gggttagttt ttcgtgcccc aggttaccgt
     2281 tgtccagcag ttccatatca aaacctgacg cggtacccag ttcagccacc gcgggtaagt
     2341 tgaacgggaa gacgacggct ttattgatac tgcttagcgc aatcattgcc cgctgaatga
     2401 tcgcggtaac cgagttttcc tcaccgacac gttcagacca cggcttgaga ctgataaacg
     2461 ccagaccgtt gttttgcccc tggccgctga agccaaagcc gccaacggta aacaccgact
     2521 gaacattatc tttctctttt gtaagataat aatccgtcac ctgttgcagc actttcgtgg
     2581 tgttaaccat ggtggcaccg gaaggtaact gcgcggtggt cataaatacc ccctgatcct
     2641 cttctggtaa gaaagaggtc ggcgtgcgca ggaacagcac cgccatcccg gcgcaaatca
     2701 gcaggtagat caccatgtag cgaccggtac aacgcaacag tgagcgggtg ctatcggtat
     2761 agtgctgagt tgatttttca aacagcgtgt tgaagcgtgc gaacagggca ttaggtttgt
     2821 gaccgccttc cggcgcggct ttcagaatgg tggcgcacag ggcaggggtc aggctcattg
     2881 ccacaaatac tgaaagcagc atggaggaga tcagcgtgat ggagaactgg cggtagatct
     2941 ccccggttgc accgctcata aaggccatcg gcataaacac tgcggaaaga acaacggcaa
     3001 taccgaccag cgcacgttgg atctgcccca tcgatttatg cgtcgcttcc ttcggtggta
     3061 gcttatcttc cgcaatgaca cgctcgacgt tctccaccac cacgatggcg tcatccacca
     3121 gtaaccctat cgccagcacc atcccgaaca tagtcaacgt gttgatggtg aaaccgaccg
     3181 ccgacaagat cgcaaacgtc ccgagaataa ccaccggtac ggcaatcgtc gggatgattg
     3241 tggcacggaa attctgcaaa aacagataca tgaccaggaa gacgaggatg atagcctcaa
     3301 ccagtgtttt gaaaacttcc tgaatagaaa tttcgataaa cggcgtggtg tcgtaaggat
     3361 aaaccgtctt cagacttgcc gggaaatagg ctgataagcg gttcagttcc tctttgactg
     3421 cccgcgaggt atccagggcg tttgctccgg tagccagttt gatggcgatc ccggcagcag
     3481 gtttgccgtt atagcgcgcc acggtggaat aatcttccgc cccaagttcg acgcgggcga
     3541 catcacgcag cagcacttgc gaaccatctt gctgaacttt caacaggatt ttgccaaatt
     3601 cttccggcgt ttgcagacgc gtctgcacaa tgatcgaggc gtttagttgc tggtctgccg
     3661 cctgtggcat gccacccagt tgaccaccag aaatctggtt gttttgcacc ttaatctggg
     3721 aaataacatc ggaaggtacc agattgtatt tattgagttt ttgcgggtcc agccagatac
     3781 gcatggcgta ctcggaacca aagagctgta cgctaccaac gcccgcggta cggcttagcg
     3841 ggtctttgat attagacgct acatagtccg cgatatcgta ctggttgagg ctgccgttat
     3901 cagaaataaa cgccgctacc atcaggatat tactgctcga tttatcgacg ctaatcccct
     3961 gctgctgcac tgcttcaggt aatgaaggca tagcgagttg cagtttattt tgcacctgaa
     4021 cctgtgcgat atcaggagat gtcccagtct cgaaggtcag agtgatagag gcattacccg
     4081 ccgcatcact ggttgaagac atgtacatca ggccatcaag cccattcata ttttgctcaa
     4141 tcacctgagt gaccgagtct tctaccgttt gcgcatcggc acccggatag gtagcgctga
     4201 cggtaatggt cggtggcgca atctgcggat actgcgcaac cggtaagttc atgatcgcca
     4261 gaccacctgc aagcatcata ataatggcaa gtacccaggc aaaaaccggg cgatcaataa
     4321 aatagttagc catgaaagtc cccttaagcc tgcaacgtta ttgtttcgat tcggtgctgg
     4381 cgttttcctg gctggaggaa attgctcgtg ctttgatacc cggacgaatg cgttgcaaac
     4441 cggaaacgat cacccgatcg ccagcctgca agccagaggt gacaacccac tggtcgccga
     4501 tggctttgct ggcttcaatt tcgcgtagct tcacgacatc gtctttatcc agaatgagcg
     4561 ccgttgcttt accttgggcg ttgtgggtga cgccttcctg cggcaccagt aatacattct
     4621 ggcggctacc ttcatccact aatgccgtga catacatgcc aggcagcaag tcaccatttg
     4681 ggttggggaa aatcgcccgt aacgtcacgg agcccgtggt ttcatcaacc gtcgggtcgg
     4741 agaatttcag cgtgccggtc tggctgtaac gtttaccatt ttccagattg agctgtactg
     4801 gcgtactgcc ctgaacctgt ttgatttgcc cactggcgac ctcttctttc atgcgtaaga
     4861 aatcttgcac cgactgcgtg agatcgacat aaatcgggtc cagacgttgt acggtaacca
     4921 gcgaatctgc ctgattagcg gtaacgagtg cgccgacggt caccgacgat ttcccgctga
     4981 cgcccgtaat cggcgaggtg acattcgcgt attgcagatt gatcgtcgcc tgttcaacag
     5041 ccgctttggc gacggtgaca ttggcttctg cttcattcaa ctgggtgcgc gcggtgtcgt
     5101 aatcctgacg gctaacgtag ttggtcttca gcaacgatgc ctggcggtta aaggtgatgc
     5161 gggcattgct ggcggtagag agcgctttcg ccagcgagcc tttggcggag tttagctcgg
     5221 cctgtaaagg tgcaggatca atctgataca gcgaatcgcc ctggttcact ttatcgcctt
     5281 cgataaagtt gcgtttaatg ataataccgc ccacctgggg acgtatctcg gcaacttcat
     5341 aaggaacggt tctaccgggc aattcgctca acacattgac cgacccaggg gagagtgtga
     5401 cgacaccgac ctcaggcgtc atggcggcgg tgttttccgc cgatttgtca tcgcaggcgg
     5461 tgagcatcgc gccgcagaat aacaacggta ttaacagctt tcttcttctg ttcattttag
     5521 tccctgaaaa ttcttgagaa ataacggttc tttaaattgc gcgcgaggag agatatgagg
     5581 gatttacaga ccagcggcat agtggggttg tcagccagta aggtgggata caggcatagt
     5641 gatcgacatg gtgaggtcaa cgacaaaaaa aatgttgccg ttctgccaac agttcctgcc
     5701 agcattcggg caacgaaggg ttctactggt ggatacacat accaggggaa taaagtcgtt
     5761 ttagcctctc ctttagtaat caccgggggt aatgaaatca gcatctgcat ccctcgtcat
     5821 gccagccatc aatttcagtt gcttatgtcc tgactaaaaa taagatgtga tacccagggt
     5881 gacgatgtcg ctcatacgtc ttagtttcag cttgtacata atgttgaatt tctgaatctt
     5941 taatgtttgt tgcgaaatat tgagcgtcga cgtgatattg ctttcatttt tatttttcag
     6001 aatcagttgc ataattttga actgattatg tgaaaa
//
LOCUS       DAZKIP010000201         5992 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZKIP010000201 DAZKIP010000000
VERSION     DAZKIP010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5992
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.203"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(69..830)
                     /gene="kduD"
                     /locus_tag="ACHOUK_004550"
     CDS             complement(69..830)
                     /gene="kduD"
                     /locus_tag="ACHOUK_004550"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFP9899846.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(860..1696)
                     /gene="kduI"
                     /locus_tag="ACHOUK_004551"
     CDS             complement(860..1696)
                     /gene="kduI"
                     /locus_tag="ACHOUK_004551"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFP9899847.1"
                     /translation="MDVRQSIHSAHAKTLETQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIIGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTGTPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(1983..3164)
                     /gene="yqeF"
                     /locus_tag="ACHOUK_004552"
     CDS             complement(1983..3164)
                     /gene="yqeF"
                     /locus_tag="ACHOUK_004552"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFP9899848.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVVDTDEQ
                     PHTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3419..4648
                     /gene="yqeG"
                     /locus_tag="ACHOUK_004553"
     CDS             3419..4648
                     /gene="yqeG"
                     /locus_tag="ACHOUK_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9899849.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            5108..5740
                     /gene="yqeH"
                     /locus_tag="ACHOUK_004554"
     CDS             5108..5740
                     /gene="yqeH"
                     /locus_tag="ACHOUK_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9899850.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWTEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
BASE COUNT         1665 a         1430 c         1252 g         1645 t
ORIGIN      
        1 gattatgatg atctagagat ataaaaaacc ctgccatgcg gcagggtcat aaaagtaaga
       61 ggaatgaatt aacgcgccag ccaaccgcca tccacagcaa tggtataacc atttacataa
      121 tctgaagcgc tggaggcaag aaacactacc ggccccatca ggtcactcgg cagtccccaa
      181 cggccagctg gaatgcggtc gagaatttcc gcgctacgtt gttcatctgc ccgcagttgt
      241 tgggtattgt tggtcgccat gtaacccgga gctatcgcat taacattaat gttgtgttta
      301 gcccattcat tcgccatcaa tcgcgtcacg cccatcacgc cgctttttga tgcggtataa
      361 gaaggcacac ggatcccgcc ctggaaggag agcattgacg cgatattgat aatcttgccg
      421 ccattgcctt gcgcgatgaa gtgtttcgcc gctgcctgag acatgaagaa tacgctcttg
      481 atattcaggt tcatgacatc gtcccagtcc ttttcgctga actcgagagc atcttcgcgg
      541 cgaatcaatc cggcgttatt caccaggata tcaatatgac caaactcagc taccgcgcga
      601 tccagcagtc caggaatacc atcaatcttt cgcagatcgg cggtcaggct taaaaaacga
      661 cgccccagcg ccgtgacctg cttgatggtt tcagtcggtt caacgatgtt aatgccaaca
      721 atgtcacagc ccgcttgcgc cagccccaac gccatcccct ggcccagccc agtatcacaa
      781 ccagtgacga ccgcaacttt accttcgaga gaaaatgcac ttaaaatcat aacaatacct
      841 tattcgttta tgcccacaac tagcgcaaat ctttaacggc cacatggtcc atatcatcaa
      901 agacctggtt ttcaccgacc atgccccaga taaaggtata agctttggtt ccgacgccgg
      961 aatgaatcga ccagctcggg gagatcaccg cctgctcgtt atgcatcaca atgtgacgcg
     1021 tttcttgcgg ctgccccatc atgtggaaaa cgcaggcgtc atcatccata ttgaaataga
     1081 aataaacttc catccggcgc tcgtgggtgt gacacggcat ggtgttccac aagttacccg
     1141 gagccagctc cgtcagcccc atactcaatt ggcaggtttc cagcacatcc gggacaaaat
     1201 atttgttaat cgtgcgacgg ttactggtga ggttatcgcc taacgtgact ggagatactt
     1261 cgtccggtgt gacttttttg gtaggatacg tcgtatgcgc gggtgcgcaa ttgtaataaa
     1321 acttcgccgg agtgccggta tcaatactag caaagacaac ttcttttgcg cctttaccca
     1381 catacagggc gtcgcggtga ccgatttcat agcattgacc atcgacagta atcgtaccgg
     1441 caccgccaat attgataaca cctaactcgc gacgttcaag gaaataactt acgccgagtt
     1501 gtttacccac ttccccgcca acggaaaccg ttttagttac cggcataatg ccgccaataa
     1561 taatacggtc aatgtggctg taaaccatgg tgtactcatc ggcgacaaat accttttcaa
     1621 ccaaaaattc attgcgcagc ccctgggttt ccagcgtttt tgcgtgcgca ctgtggatgc
     1681 tctgtcttac gtccacatca acctccgata atcagtgaag cgtgaaaata gaaaacgata
     1741 gtgaaacact gtttcgtttt ctctataagg ggatgatatg cccatgggat cgggttttca
     1801 attaaaaata aaacaatgtt tcatttttaa gttaaggatt aaaaaaagtg cagtgtcgat
     1861 cacgaatctg tcatgagaat ggaaaaatca gggagcccgg aaaatgtatg tcgacttgcc
     1921 agccatcatt attcaacaga ttaatgacgg cagatattta tcggtaagga ggaaatgcag
     1981 agctattcgt cacgttcaat ggtcaatgcc acaccctggc ccccgccgat acaaagcgtt
     2041 gccagtcctt tgcgggcatt acgtttcacc atttcatgaa ccagagaaac caggattcgg
     2101 caaccggaag cgcctatcgg gtgaccgagt gcgatcgcgc caccattgac attgacccga
     2161 cgctcatccc actcaagcat cttgccaacc gaaagcgcct gtgcagcaaa cgcttcatta
     2221 gcctcgataa gatcaacatc agccaactgc cagcctacac gctccaggca acggcgggtc
     2281 gcatacaccg gcgcaattcc catcaatgcc ggatctacac caacgctggc aaatgcgcgg
     2341 atgcgggcca gcacgggtaa attcaacgct cgtgctttgg cttcgctcat catcattacc
     2401 gcagctgcgc catcgtttat ggatgatgca ttacccgctg tcacagaacc gagactatca
     2461 aatgaaggat ttaaacgggc taaaccttct gcactggcgt cagtgtgtgg ctgttcatcg
     2521 gtatcaacaa ccaacgtctg accgttactt tgggtcatta ccgggacgat ctcatcttta
     2581 aatcgtccgg cgtcaatcgc cgctcgcgct ttttgttgcg agctaagtgc gtaagcatcc
     2641 tgcaattgac ggctgatgcc atattcgcga gccagatttt cggcggtgac accaatatga
     2701 taatcattga aggcatccca taacccatca tgcacaagac tgtcaaccag ctggctattg
     2761 ccaagctgtg caccggtgcg gctatcagtc agaacatgtg gtgcgcggct catgttttcc
     2821 tggccaccgg cgatgacaat atcagcctcg ccacactgta tcgcctgagt agccagatgc
     2881 agtgctttaa gcccggaacc gcagacgtca ttaatcgtga ttgcagaaac gctattaggc
     2941 agaccacctt taatagccga ttgccttgcc ggattctgcc ctgcccctgc agtcaacacc
     3001 tgaccaagaa ttacttcatc caccgcatat gcaggaacgc cggtacgttc tattaacgct
     3061 ttcacgacca gactaccaag ttccacggcg gaatgacccg ctaacgcacc gcgaaagcag
     3121 ccgataggtg tccgtaacgc cccgacaatc acaacgtctt tcatcacaac ctcgcaccaa
     3181 ttaacagtgc aatagtaaat gattgttaat tacagttatc ttaattgttt aaaaaaagtg
     3241 atttttatca caaaggaaat atacctgagc agtagtcaga gtcataactg gcacgtaagg
     3301 tttgcaacca ctaccccacc aatagagggg tagatagggc gttaatctcc catacttaac
     3361 ctggtttatg gtaaattgcc ctccattttg tttaatttgt agatgatacg ttcagataat
     3421 gtctaatatt tggtctaaag aagaaactct gtggagtttc gcgctctacg gcacagccgt
     3481 tggtgcaggc acgctcttcc ttcctattca gttaggttcg gcaggggctg tggtcctgtt
     3541 tattactgct ctggtcgcct ggcctttaac atactggcca cataaagctt tatgccagtt
     3601 catcctctca tcgaaaacat cagcaggtga agggataacg ggcgcggtaa cacactacta
     3661 tggcaagaag attggtaatc tgattaccac gctgtacttc atcgcctttt ttgtcgtcgt
     3721 gttgatatat gcagtggcaa ttaccaactc acttacggaa cagctggcaa agcatatggt
     3781 tattgatctt cgcatccgta tgttggtgag tctgggtgtt gtattaattc tgaatctcat
     3841 ttttctgatg ggacgtcatg ccactattcg ggtaatggga tttttggtat tcccattgat
     3901 tgcctatttc ttatttcttt ccatttacct tgtaggtagt tggcaacctg atctattaac
     3961 aacccaggta gagttcaatc agaataccct tcaccagata tggatatcga ttcccgtgat
     4021 ggttttcgcc tttagccata cgcccattat ttctacgttt gccatagaca gacgtgaaaa
     4081 atatggcgaa cacgctatgg ataaatgcaa aaaaattatg aaagtcgctt atctcatcat
     4141 ctgcataagt gtactgttct ttgtctttag ctgcctgctt tctattccac cttcgtatat
     4201 tgaagcggct aaagaagaag gggtcaccat tttatcggcg ctttctatgc tgccgaacgc
     4261 cccagcatgg ttgtcaattt ccgggattat tgtcgcagta gttgcgatgt cgaaatcatt
     4321 cctgggtacg tactttggcg ttattgaagg tgccacggag gtcgtcaaaa caacactaca
     4381 gcaggttggt gtaaagaaaa gtcgtgcatt taaccgcgca ctatcaatta tgttggtatc
     4441 gctgattacc ttcattgttt gttgcattaa cccgaacgcg atttcgatga tttatgcgat
     4501 cagcggcccg ctcattgcca tgatactttt catcatgcct acgctatcaa catatctcat
     4561 cccggcgctt aaaccctggc gctccatcgg aaatctgatt acgctgatcg tgggtatcct
     4621 gtgcgtatcg gtaatgttct ttagctaata tccattgtga atggttcaaa attgctacaa
     4681 ttttgaacca ttccagtttt cataatgtgc atttgttatt attccgctct atcacttctc
     4741 tcgtcatatc ctagtattat cagttagtta aacacacaaa gagatctgaa catcttaaat
     4801 aagactattt aagatgcata acttagattc gcaagatatc ttctggtcat tcaaaaacaa
     4861 tttgcgatat agatattctc ataccatgag atagttatct gaaccaccct caaccaggta
     4921 agattaaatc agtgaaacat aatcatattt aataacgaga aatgcatttt aaaatgtctt
     4981 aatcagaatt atagcaaata cagattaatc gcaatttttc ccagcaaaat tacaaaatca
     5041 tggtgtattg acatcatcaa tcaccaatga aattatgcaa ttatatacgg atagggaggt
     5101 tcttaacatg gggctttgta gtcgttataa aagtcttaca tgcaatagtt gctcaatgca
     5161 ttgccaaata atgccagaag agtcaccgcg tttgcagtac tgcgcaaact cgtgtttttg
     5221 tatgtggacc gaagaaagtt catattttaa tcgtggtgtg gtagaaggta tcttaacaaa
     5281 aaaccacaat gcgagattaa gcggatatat ttttgtcgat ttttcagtaa gttttttacg
     5341 tctattcctt gaaaaagact ggattgacta tcttgcaagt actgacatgg ggattgtttt
     5401 agtcagcgac cgcaatatgc aatcgttagc taattactgg cgaaaacaca actctgcaat
     5461 atctgctgtc atatataatg atgatggtct tgatgttgcc aatgaaaaga tcagacaact
     5521 atttattggt cgttatttat catttaccag aggtaacaca ttaactcaga tggaattcac
     5581 cataatgggg tatatggttt ctggttataa tccatatcag attgctgaag ttctggatat
     5641 ggatatccgt agcatctacg cgtacaagca acgaatcgaa aagagaatgg gtggtaaaat
     5701 aaacgaatta tttattcgtt cacattcggt ccaacattga tactcaacaa tctgccagaa
     5761 tcatactctg tttatacggg catttattga aatgcccgta ttttataaat aggccgtccc
     5821 ctcaagagta aacaccatag tatttaaaac catcacacga aaaattcaga agcattacga
     5881 aatatgaact tacaaacaat agtggcataa atgttaacca tgttaattta cataaaattt
     5941 tacgttgcga cattaaagcc tcatttcaat catcatgata aatataaaat ta
//
LOCUS       DAZKIP010000202         5965 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZKIP010000202 DAZKIP010000000
VERSION     DAZKIP010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5965
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.204"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            219..866
                     /gene="fucA"
                     /locus_tag="ACHOUK_004555"
     CDS             219..866
                     /gene="fucA"
                     /locus_tag="ACHOUK_004555"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFP9899851.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            894..2042
                     /gene="fucO"
                     /locus_tag="ACHOUK_004556"
     CDS             894..2042
                     /gene="fucO"
                     /locus_tag="ACHOUK_004556"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFP9899852.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(2097..2852)
                     /gene="xni"
                     /locus_tag="ACHOUK_004557"
     CDS             complement(2097..2852)
                     /gene="xni"
                     /locus_tag="ACHOUK_004557"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFP9899853.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(2964..4331)
                     /gene="sdaB"
                     /locus_tag="ACHOUK_004558"
     CDS             complement(2964..4331)
                     /gene="sdaB"
                     /locus_tag="ACHOUK_004558"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFP9899854.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMCG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(4389..5678)
                     /gene="sdaC"
                     /locus_tag="ACHOUK_004559"
     CDS             complement(4389..5678)
                     /gene="sdaC"
                     /locus_tag="ACHOUK_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFP9899855.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         1498 a         1477 c         1590 g         1400 t
ORIGIN      
        1 ttttgataaa tatttttaag agcggtcatt tttaatgggg ttataaaaag agaacttgca
       61 gttatttact gtgattagtt gaactaggtc acaaaatata agcttgtgaa tttctttctt
      121 gttaatcagg aagaagatgg tgggtaatta aacggctaat tcaatagtgt gaaaggaaca
      181 acattattgc cctgttttga atcagagaga ggtagctaat ggaacgaaat aaacttgctc
      241 gtcagattat tgacacttgc ctggaaatga cccgcctggg actgaaccag gggacagcgg
      301 ggaacgtcag tgtacgttat caggatggga tgctgattac gcctacaggc attccatatg
      361 aaaaactgac ggagtcgcat attgtcttta ttgatggcaa cggtaaacat gaggaaggaa
      421 agctcccctc aagcgagtgg cgtttccata tggcggccta tcaaagcaga ccggatgcca
      481 acgcggttgt tcacaatcat gccgttcatt gcacagcagt ttccattctt aaccgaccga
      541 tccccgctat tcactacatg attgcggcgg ctggcggtaa ttctattcct tgcgcgcctt
      601 atgcgacctt tggaacacgc gaactttctg aacatgttgc gctggctctc aaaaatcgta
      661 aggcaacttt gttacaacat catgggctta tcgcttgtga ggtgaatctg gaaaaagcgt
      721 tatggctggc gcatgaagtt gaagtgctgg cgcaacttta cctgacgacc ctggcgatta
      781 cggacccggt gccagtgctg agcgatgaag agattgccgt agtgctggag aaattcaaaa
      841 cctatgggtt acggattgaa gagtaatttc gtaaagcaac aaggagaagg atgatggcta
      901 acagaatgat tctgaacgaa acggcatggt ttggtcgggg tgctgttggg gctttaaccg
      961 atgaggtgaa acgccgtggt tatcagaagg cgctgatcgt caccgataaa acgctggtgc
     1021 aatgcggcgt ggtggcgaaa gtgaccgata agatggatgc tgcagggctg gcatgggcga
     1081 tttacgacgg cgtagtgccc aacccaacaa ttactgtcgt caaagaaggg ctcggtgtat
     1141 tccagaatag cggcgcggat tacctgatcg ctattggtgg tggttctcca caggatactt
     1201 gtaaagcgat tggcattatc agcaacaacc cggagtttgc cgatgtgcgt agcctggaag
     1261 gactttcccc gaccaataaa cccagtgtac cgattctggc aatccccacc acagcaggca
     1321 ctgcggcaga agtgaccatt aactacgtga tcactgacga agaaaaacgg cgcaagtttg
     1381 tttgcgttga tccgcatgat atcccgcagg tggcgtttat tgacgctgac atgatggatg
     1441 gtatgcctcc agcgctgaaa gctgcgacgg gtgtcgatgc gctcactcat gctattgagg
     1501 ggtatattac ccgtggcgcg tgggcgctaa ccgatgcact gcacattaaa gcgattgaaa
     1561 tcattgctgg ggcgctgcga ggatcggttg ctggtgataa ggatgccgga gaagaaatgg
     1621 cgctcgggca gtatgttgcg ggtatgggct tctcgaatgt ggggttaggg ttggtgcatg
     1681 gtatggcgca tccactgggc gcgttttata acactccaca cggtgttgca aacgccatcc
     1741 tgttaccgca tgtcatgcgt tataacgctg actttaccgg tgagaagtac cgcgatatcg
     1801 cgcgcgttat gggcgtgaaa gtggaaggta tgagcctgga agaggcgcgt aatgccgctg
     1861 ttgaagcggt gtttgctctc aaccgtgatg tcggtattcc gccacatttg cgtgatgttg
     1921 gtgtacgcaa ggaagacatt ccggcactgg cgcaggcggc actggatgat gtttgtaccg
     1981 gtggcaaccc gcgtgaagca acgcttgagg atattgtaga gctttaccat accgcctggt
     2041 aaatgcgctg atgtgataat gccggatgcg acgtttgcgg cgtatccggc acgccgttac
     2101 cgtaccaacc gcaattgctg taaattgccg tcgatatgca aatcggtttg taagcgggca
     2161 atatcgcggc acagaaacgc catctcttta tgggtttcta attttttgcg ccacttttcg
     2221 gcaaccgcat ccagattctc atatatccct tccagactct gaaactcgac cagcagctgc
     2281 gtggcgcttt ttggtccgat tcccgcaaca cccggtacct ttgaactgct gatccccgca
     2341 agtccccagt aatcgggcaa ctgctgcggt tgaacgccaa attctttatc gataaatggc
     2401 gcatccagcc aacgtttctg gaagtaatca cgaatacgta atgtcggtga aagtaactga
     2461 cagtagcctt tatctgtcga aacaatcgtt gcctgatgcc cggcctgtgt cactttgacc
     2521 gccagcgtgg cggctaagtc atcggcttcg ttgccgctgg ttgaccagca cgggacgccg
     2581 cgttgctcaa aggcggcgcg taatgcaggc atctcgtcgt gcaactcttc cggcattggc
     2641 ggtcgacccg ctttgtaatc tggtaaacgc tgatgacgcc agccgctact gcggttttca
     2701 tcgtcaaaaa cggcgaccgc gtgggttggc tggctgtgca taatgagctg atcgagcgca
     2761 tgctggcagg tttcgacaca gggcgacccc tgaacggcat gaatgcgacg aataagattc
     2821 agtgcatcga caataagcaa atgaacagcc acgataaccc ccttaaagat caggcgataa
     2881 gggtaacatt cgatactgga acaggctatg gctggatgag aaatcgggaa gaggcctcgc
     2941 aaaacgaggc ctctggagag cgattaatcg caggcaacga tcttcattgc caggccgccg
     3001 cgagaggttt cgcggtactt ggcgttcata tctttacctg tttcgtacat ggtttcgata
     3061 actttatcga ggcagacgcg cggctcgctg gtacggcgca gcgccatacg tgcggcgttc
     3121 accgctttta ctgccgcaat ggcgttacgc tcgatgcatg gcacctgtac ctgtccggcg
     3181 accgggtcac acgtcagacc gaggttgtgc tccatggcga tttccgccgc gatgcacacc
     3241 tgcgccgggc ttgcacctaa cagttctgcc agaccagccg ccgccattga gcacgccaca
     3301 ccaacttcac cctggcaacc cacttcggca ccagaaatcg acgcgttcat cttataaaga
     3361 gaaccaatgg cgctggctac cagcaggtaa cgagccagtg agttagcgtt cacttcgcgg
     3421 ataaacttat cgtagtacgc cagcactgcc gggataatcc cgcacgcacc gttagtcggc
     3481 gcagtcacca cgcgaccgcc agcagcgttc tcttcgttca ctgccagtgc aaacatgttg
     3541 atccagtcaa caaccgccat cgggtcagtg gtggttttat cctggctgac cagcatccgg
     3601 cgtagtgccg cagcacggcg tggaacacgc agtttgccag gtaacacacc ttcggtggaa
     3661 ataccgcgct caataccgcc acacatcact tcccagacgt tcgccaggtg ctgttccagc
     3721 tcttctttgc tgtgcagcgc cagctcgttt ttcatcatca ggccagagag tgacagcccg
     3781 gtttcctgac aatgtttttg cagatcggct gctgaactgt acggataagg aacttcaacc
     3841 ggtgcgctat cctgctggcc aaaatgctct tcatcaacga taaagccacc gccaatagag
     3901 tagtaagtct gactgtaaac gactttatca cccgccagcg cggtaatgcg cataccgttt
     3961 tcatgcagag aaaggttgtc ggcatggaag ttcatgcact gatcaaccgg gaactccact
     4021 tcatgctgac cgtttgccag catcaggcga ccatgagtat tcacatcctg aataaaacca
     4081 gggatggaat cgatatccac ggtatccggc aggttacccg ccaggcccat aataatggcg
     4141 atatcagtgt ggtggccttt accagtcaga gagagcgagc cgtacacgtc aaccaccacg
     4201 cgggtcacgt ctttaagcag gttacgggca atcagatcgt cggtaaattg tttacccgct
     4261 ttcattggtc caacggtatg agaactggaa gggccaatgc cgattttgaa aatatcgaat
     4321 acgctaatca taggaaatac atcgcgttaa aacggaggaa gcgccgcccg aaagcggcgc
     4381 gaaaggactt agctgaacag agagtagaag attgcggaga ttgcaatcag acccatcacg
     4441 acaacgaata cgttgctgat gtgaccgctg tacttacgca ttgccggtac tttctgaatt
     4501 gcgtacatcg gcatcaggaa caggatcatc gcgatgattg ggccgcccag ggtttcaatc
     4561 atacccagga tgctcgggtt cagggtggca acaatccagg tcgttaccag catgaacagc
     4621 gcagtgatac ggttcagctt gttgatttcg atagacttac ctttaccacg cagagattta
     4681 atcaccatac cgttgaagcc ttcacgtgcg cccaggtagt ggccgaggaa ggatttggtg
     4741 atagcgataa tcgcgataat cggagccatc cacgcgataa ccggtgcgtt aaagtggtta
     4801 gccaggtaag acagaatcga gatgttctgc tctttagccg cagccaggtc tgccggagtc
     4861 aggctcagta cgcagctgaa gacgaagaac attacggtca gcaccatcat gatgtgtgcg
     4921 aatgccagga tcttcgagca tttctgttct gccatatcgc cgtactcttc acgcttcgca
     4981 acggcgaaag aagagatgat cggagagtgg ttgaacgaga acaccattac cggaattgcc
     5041 agccacaggg tcatccacag accgtttccg gttgcagatg cagtgtccag agacagcgtt
     5101 tccagtgctg cgccgttcca ctgcgggatc aggtacagag ccagcagcat cagtacgcca
     5161 acaaacggga ataccagaat actcatcgct ttaacgatca tctgttcacc aaagcgaacg
     5221 atggtcatca tacccacgat caggatcagc gacagaatcg cacgcggcgg tggtgtcata
     5281 cccagctggt gagacatgaa gctttcaact gtattggtga ttgccacgct ataaaccagc
     5341 aggatcgggt agatagcgaa gaagtagagc agggtaatca gtttacctgc gccaatacca
     5401 aagtgttctt ctacaacctc ggtaatgtct tcgcccgggt ttttaccaga cagtacgaag
     5461 cgagtcaggc cgcggtgagc aaaaaacgtc atcgggaacg caaggatagc catgatgatc
     5521 agcgggatca taccgccaac accggcgttg attggcagga acagcacgcc cgcgccgatt
     5581 gccgtgccgt aaaggcccag catccacatg gtgtctgtct tgcgccaggc actacgagag
     5641 tctttcgacg caatcgtgct ggtttgagtc gtttccatct atttctcctg gaggaatgca
     5701 aataattcag cttttagcca gttcagcccg ggaaggtgcc tgaccgtaaa aatgaaatct
     5761 gtctatcggc gatggcttaa tcatttaccg ccgtaataat ttgagggcgg aaagatacac
     5821 acaagttatg tatctatcag tgatcttgat cccaaataca ataatctact tggaagatag
     5881 attatcccaa cgctttactc tgtaaatcaa aagtaaaaga gaatttaagg gcaatatttg
     5941 cccattaaac gacatctgac ttctt
//
LOCUS       DAZKIP010000203         5923 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZKIP010000203 DAZKIP010000000
VERSION     DAZKIP010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.205"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..422
                     /locus_tag="ACHOUK_004560"
     CDS             <1..422
                     /locus_tag="ACHOUK_004560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP9899856.1"
                     /translation="PGYFNELMVLAKKLGIESKIKILGHIPKLEQIELIKNCIAVIQP
                     TLFEGGPGGGVTFDAIALGKKVILSDIDVNKEVNCGDVYFFQAKNHYSLNDAMVKADE
                     SKIFYEPTTLIELGLKRRNACADFLLDVVKQEIESRS"
     gene            442..1560
                     /gene="gmd"
                     /locus_tag="ACHOUK_004561"
     CDS             442..1560
                     /gene="gmd"
                     /locus_tag="ACHOUK_004561"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFP9899857.1"
                     /translation="MTKVALITGVTGQDGSYLAEFLLDKGYEVHGIKRRASSFNTERI
                     DHIYQDPHGSNPNFHLHYGDLTDSSNLTRILKEVQPDEVYNLAAMSHVAVSFESPEYT
                     ADVDAIGTLRLLEAIRFLGLENKTRFYQASTSELYGLVQEIPQKESTPFYPRSPYAVA
                     KLYAYWITVNYRESYGIYACNGILFNHESPRRGETFVTRKITRGLANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVRMQWLMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKMSF
                     VGKGIEEKGIVDSVEGQDAPGVKPGDVIVAVDPRYFRPAEVDTLLGDPSKANLKLGWR
                     PEITLAEMISEMVAKDLEAAKKHSLLKSHGFSVSLALE"
     gene            1563..2528
                     /locus_tag="ACHOUK_004562"
     CDS             1563..2528
                     /locus_tag="ACHOUK_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase family protein"
                     /protein_id="HFP9899858.1"
                     /translation="MNKQRIFIAGHQGMVGSAITRRLKQRDDVELVLRTRDELNLLDS
                     SAVLDFFSSQKIDQVYLAAAKVGGILANSSYPADFIYENIMIEANVIHAAHKNNVNKL
                     LFLGSSCIYPKLAHQPIMEDELLQGKLEPTNEPYAIAKIAGIKLCESYNRQFGRDYRS
                     VMPTNLYGPNDNFHPSNSHVIPALLRRFHDAVENNSPNVVVWGSGTPKREFLHVDDMA
                     SASIYVMEMPYDIWQKNTKVMLSHINIGTGIDCTICELAETIAKVVGYKGHITFDTTK
                     PDGAPRKLLDVTLLHQLGWNHKITLHKGLENTYNWFLENQLQYRG"
     gene            2531..3040
                     /locus_tag="ACHOUK_004563"
     CDS             2531..3040
                     /locus_tag="ACHOUK_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFP9899859.1"
                     /translation="MFLHSQDFATIVRSTPLISIDLIVENEFGEILLGKRINRPAQGY
                     WFVPGGRVLKDEKLQTAFERLTEIELGIRLPLSVGKFYGIWQHFYEDNSMGGDFSTHY
                     IVIAFLLKLQPNILKLPKSQHNAYCWLSRAKLINDDDVHYNCRAYFNNKTNDAIGLDN
                     KDIICLMRQ"
     gene            3022..4470
                     /locus_tag="ACHOUK_004564"
     CDS             3022..4470
                     /locus_tag="ACHOUK_004564"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HFP9899860.1"
                     /translation="MSDAPIIAVVMAGGTGSRLWPLSRELYPKQFLQLSGDNTLLQTT
                     LLRLSGLSCQKPLVITNEQHRFVVAEQLREINKLNGNIILEPCGRNTAPAIAISAFHA
                     LKRNPQEDPLLLVLAADHVIAKESVFCDAIKNATPIANQGKIVTFGIIPEYAETGYGY
                     IERGELSVPLQGHENTGFYYVNKFVEKPNRETAELYMTSGNHYWNSGIFMFKASVYLE
                     ELRKFRPDIYNVCEQVASSSYIDLDFIRLSKEQFQDCPAESIDFAVMEKTEKCVVCPV
                     DIGWSDVGSWQSLWDISLKSKTGDVCKGDILTYDTKNNYIYSESALVAAIGIEDMVIV
                     QTKDAVLVSKKSDVQHVKKIVEMLKLQQRTEYISHREVFRPWGKFDSIDQGERYKVKK
                     IIVKPGEGLSLRMHHHRSEHWIVLSGTAKVTLGDKTKLVTANESIYIPLGAAYSLENP
                     GIIPLNLIEVSSGDYLGEDDIIRQKERYKHED"
     gene            4474..5844
                     /gene="cpsG"
                     /locus_tag="ACHOUK_004565"
                     /gene_synonym="manB"
     CDS             4474..5844
                     /gene="cpsG"
                     /locus_tag="ACHOUK_004565"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFP9899861.1"
                     /translation="MKSLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSEALKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGEMVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVKLMEKKTKAL
                     LKLLSE"
BASE COUNT         1771 a         1121 c         1334 g         1697 t
ORIGIN      
        1 tccctggata ttttaatgaa ttgatggttt tggcaaaaaa gctcggaatt gaatcgaaaa
       61 ttaagatatt agggcatata cctaaacttg aacaaattga attaatcaaa aattgcattg
      121 ctgtaataca accaacctta tttgaaggcg ggcctggagg gggggtaaca tttgacgcta
      181 ttgcattagg gaaaaaagtt atactatctg acatagatgt caataaagaa gttaattgcg
      241 gtgatgtata tttctttcag gcaaaaaacc attattcatt aaatgacgcg atggtaaaag
      301 ctgatgaatc taaaattttt tatgaaccta caactctgat agaattgggt ctcaaaagac
      361 gcaatgcgtg tgcagatttt cttttagatg ttgtgaaaca agaaattgaa tcccgatctt
      421 aatatattca agaggtatat aatgactaaa gtcgctctta ttacaggtgt aactggacaa
      481 gatggatctt atctagctga gtttttgctt gataaagggt atgaagttca tggtatcaaa
      541 cgccgagcct catcttttaa tacagaacgc atagaccata tttatcaaga tccacatggt
      601 tctaacccaa attttcactt gcactatgga gatctgactg attcatctaa cctcactaga
      661 attctaaagg aggtacagcc agatgaagta tataatttag ctgctatgag tcacgtagca
      721 gtttcttttg agtctccaga atatacagcc gatgtcgatg caattggtac attacgttta
      781 ctggaagcaa ttcgcttttt aggattggaa aacaaaacgc gtttctatca agcttcaacc
      841 tcagaattat atggacttgt tcaggaaatc cctcaaaaag aatccacccc tttttatcct
      901 cgttcccctt atgcagttgc aaaactttac gcatattgga tcacggtaaa ttatcgagag
      961 tcatatggta tttatgcatg taatggtata ttgttcaatc atgaatctcc acgccgtgga
     1021 gaaacgtttg taacaaggaa aattactcga ggacttgcaa atattgcaca aggcttggaa
     1081 tcatgtttgt atttagggaa tatggattcg ttacgagatt ggggacatgc aaaagattat
     1141 gttagaatgc aatggttgat gttacaacag gagcaacccg aagattttgt gattgcaaca
     1201 ggagtccaat actcagtccg tcagtttgtc gaaatggcag cagcacaact tggtattaag
     1261 atgagctttg ttggtaaagg aatcgaagaa aaaggcattg tagattcggt tgaaggacag
     1321 gatgctccag gtgtgaaacc aggtgatgtc attgttgctg ttgatcctcg ttatttccga
     1381 ccagctgaag ttgatacttt gcttggagat ccgagcaaag ctaatctcaa acttggttgg
     1441 agaccagaaa ttactcttgc tgaaatgatt tctgaaatgg ttgccaaaga tcttgaagcc
     1501 gctaaaaaac attctctttt aaaatcgcat ggtttttctg taagcttagc tctggaatga
     1561 tgatgaataa gcaacgtatt tttattgctg gtcaccaagg aatggttgga tcagctatta
     1621 cccgacgcct caaacaacgt gatgatgttg agttggtttt acgtactcgg gatgaattga
     1681 acttgttgga tagtagcgct gttttggatt ttttttcttc acagaaaatc gaccaggttt
     1741 atttggcagc agcaaaagtc ggaggtattt tagctaacag ttcttatcct gccgatttta
     1801 tatatgagaa tataatgata gaggcgaatg tcattcatgc tgcccacaaa aataatgtaa
     1861 ataaactgct tttcctcggt tcgtcgtgta tttatcctaa gttagcacac caaccgatta
     1921 tggaagacga attattacaa gggaaacttg agccaacaaa tgaaccttat gctatcgcaa
     1981 aaattgcagg tattaaatta tgtgaatctt ataaccgtca gtttgggcgt gattaccgtt
     2041 cagtaatgcc aaccaatctt tatggtccaa atgacaattt tcatccaagt aattctcatg
     2101 tgattccggc gcttttgcgc cgctttcatg atgctgtgga aaacaattct ccgaatgttg
     2161 ttgtttgggg aagtggtact ccaaagcgtg aattcttaca tgtagatgat atggcttctg
     2221 caagcattta tgtcatggag atgccatacg atatatggca aaaaaatact aaagtaatgt
     2281 tgtctcatat caatattgga acaggtattg actgcacgat ttgtgagctt gcggaaacaa
     2341 tagcaaaagt tgtaggttat aaagggcata ttacgttcga tacaacaaag cccgatggag
     2401 cccctcgaaa actacttgat gtaacgcttc ttcatcaact aggttggaat cataaaatta
     2461 cccttcacaa gggtcttgaa aatacataca actggtttct tgaaaaccaa cttcaatatc
     2521 gggggtaata atgtttttac attcccaaga ctttgccaca attgtaaggt ctactcctct
     2581 tatttctata gatttgattg tggaaaacga gtttggcgaa attttgctag gaaaacgaat
     2641 caaccgcccg gcacagggct attggttcgt tcctggtggt agggtgttga aagatgaaaa
     2701 attgcagaca gcctttgaac gattgacaga aattgaacta ggaattcgtt tgcctctctc
     2761 tgtgggtaag ttttatggta tctggcagca cttctacgaa gacaatagta tggggggaga
     2821 cttttcaacg cattatatag ttatagcatt ccttcttaaa ttacaaccaa acattttgaa
     2881 attaccgaag tcacaacata atgcttattg ctggctatcg cgagcaaagc tgataaatga
     2941 tgacgatgtg cattataatt gtcgcgcata ttttaacaat aaaacaaatg atgcgattgg
     3001 cttagataat aaggatataa tatgtctgat gcgccaataa ttgctgtagt tatggccggt
     3061 ggtacaggca gtcgtctttg gccactttct cgtgaactat atccaaagca gtttttacaa
     3121 ctctctggtg ataacacctt gttacaaacg actttgctac gactttcagg cctatcatgt
     3181 caaaaaccat tagtgataac aaatgaacag catcgctttg ttgtggctga acagttaagg
     3241 gaaataaata aattaaatgg taatattatt ctagaaccat gcgggcgaaa tactgcacca
     3301 gcaatagcga tatctgcgtt tcatgcgtta aaacgtaatc ctcaggaaga tccattgctt
     3361 ctagttcttg cggcagacca cgttatagct aaagaaagtg ttttctgtga tgctattaaa
     3421 aatgcaactc ccatcgctaa tcaaggtaaa attgtaacgt ttggaattat accagaatat
     3481 gctgaaactg gttatgggta tattgagaga ggtgaactat ctgtaccgct tcaagggcat
     3541 gaaaatactg gtttttatta tgtaaataag tttgtcgaaa agcctaatcg tgaaaccgca
     3601 gaattgtata tgacttctgg taatcactat tggaatagtg gaatattcat gtttaaggca
     3661 tctgtttatc ttgaggaatt gagaaaattt agacctgaca tttacaatgt ttgtgaacag
     3721 gttgcctcat cctcatacat tgatctagat tttattcgat tatcaaaaga acaatttcaa
     3781 gattgtcctg ctgaatctat tgattttgct gtaatggaaa aaacagaaaa atgtgttgta
     3841 tgccctgttg atattggttg gagtgacgtt ggatcttggc aatcgttatg ggacattagt
     3901 ctaaaatcga aaacaggaga tgtatgtaaa ggtgatatat taacctatga tactaagaat
     3961 aattatatct actctgagtc agcgttggta gccgccattg gaattgaaga tatggttatc
     4021 gtgcaaacta aagatgccgt tcttgtgtct aaaaagagtg atgtacagca tgtaaaaaaa
     4081 atagtcgaaa tgcttaaatt gcagcaacgt acagagtata ttagtcatcg tgaagttttc
     4141 cgaccatggg gaaaatttga ttcgattgac caaggtgagc gatacaaagt caagaaaatt
     4201 attgtgaaac ctggtgaggg gctttcttta aggatgcatc accatcgttc tgaacattgg
     4261 atcgtgcttt ctggtacagc aaaagtaacc cttggcgata aaactaaact agtcaccgca
     4321 aatgaatcga tatacattcc ccttggcgca gcgtatagtc ttgagaatcc gggcataatc
     4381 cctcttaatc ttattgaagt cagttcaggg gattatttgg gagaggatga tattataaga
     4441 cagaaagaac gttacaaaca tgaagattaa catatgaaat ctttaacctg ctttaaagcc
     4501 tatgatattc gcgggaaatt aggcgaagaa ctgaatgaag atattgcctg gcgcattggg
     4561 cgtgcctatg gcgaatttct caaaccgaaa accattgttt taggcggtga tgtccgcctc
     4621 accagcgaag cgttaaaact ggcgcttgcg aaaggtttac aggatgcggg cgtcgatgtg
     4681 ctggatatcg gtatgtccgg caccgaagag atctatttcg ccacgttcca tctcggagtg
     4741 gatggcggca tcgaagttac cgccagccat aacccgatgg attacaacgg catgaagctg
     4801 gtgcgcgaag gggctcgccc gatcagcggt gataccggac tgcgcgatgt ccagcgtctg
     4861 gcagaagcca atgacttccc tcctgtcgat gaaaccaaac gtggtcgcta tcagcaaatc
     4921 aatctgcgtg acgcttacgt tgatcacctg ttcggttata tcaacgtcaa aaacctcacg
     4981 ccgctcaagc tggtgatcaa ctccgggaac ggcgcagcgg gtccggtggt ggacgccatt
     5041 gaagcccgat ttaaagccct cggcgcaccg gtggaattaa tcaaagtaca caacacgccg
     5101 gacggcaatt tccccaacgg tattcctaac ccgctgctgc cggaatgccg cgacgacacc
     5161 cgtaatgcgg tcatcaaaca cggcgcggat atgggcattg cctttgatgg cgattttgac
     5221 cgctgtttcc tgtttgacga aaaagggcag tttatcgagg gctactacat tgtcggcctg
     5281 ctggcagaag cgttcctcga aaaaaatccc ggcgcgaaga tcatccacga tccacgtctc
     5341 tcctggaaca ccgttgatgt ggtgactgcc gcaggcggca ccccggtaat gtcgaaaacc
     5401 ggacacgcct ttattaaaga acgtatgcgc aaggaagacg ccatctacgg tggcgaaatg
     5461 agcgctcacc attacttccg tgatttcgct tactgcgaca gcggcatgat cccgtggctg
     5521 ctggtcgccg aactggtgtg cctgaaagga aaaacgctgg gcgaaatggt gcgcgaccgg
     5581 atggcggcgt ttccggcaag cggtgagatc aacagcaaac tggcgcaacc cgttgaggca
     5641 attaatcgcg tggaacagca ttttagccgc gaggcgctgg cggtggatcg caccgatggc
     5701 atcagcatga cctttgccga ctggcgcttt aacctgcgct cctccaacac cgaaccggtg
     5761 gtgcggttga atgtggaatc acgcggtgat gtaaagctaa tggaaaagaa aactaaagct
     5821 cttcttaaat tgctaagtga gtgattattt acattaatca ttaagcgtat ttaagattat
     5881 attaaagtaa tgttattgcg gtatatgatg aatatgtggg ctt
//
LOCUS       DAZKIP010000204         5874 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZKIP010000204 DAZKIP010000000
VERSION     DAZKIP010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.206"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..2384)
                     /locus_tag="ACHOUK_004566"
     CDS             complement(<1..2384)
                     /locus_tag="ACHOUK_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFP9899862.1"
                     /translation="MSKIFICAAIPDEQAIKEDSAVAVATAIEAGDERRARAKFHWQF
                     LEQFPAAQDCAYKFIVCEDKPGIPRPALDSWDTEYMQENRWDEESASFVPVEPESDPM
                     NVNFDKLSPEVQNAVLVKFDTCENITVDMVISAQELLQEDMATFGGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCEPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLTRA
                     RVNQQNTENAAEKTGAVTVAIRREYKQTWKTLDNELACALWPGDVDAGNIDGTIHRWA
                     TNEVIDKDREDWKRISASMRKQPEALGYDRQTIFGLVRERPIDIHKDPVALNKYISEY
                     LTTKGVFEHEETDQSSTDALQPSAAQTAPVETAESDTQKNEILVEAEPSVEREGPFYF
                     VFTDKGGEKYGRANKLSGLDKALAAGGTEISKEEYFARKNGTYTGLPQNVDTAEDSEQ
                     SEPVKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGIQSRDAE
                     DQNQPNVKQNEPEAEQNSPDTQQNGPEEQQPEPAVQQELEKVCTACGQTGGGNCPDCG
                     AVMGNATYLETFDEENQAEAQKNDPEEMEGTEHLHKENTGSDQYHASDNKTGETANPL
                     IKVNGHHEISSTSRLWHHLMIDLETMGKNPDAPINSIAGKFFDPATGEMGPEFSKTID
                     LETAGGVIDRDTIKWWLKQSREAQSSILTDEITLDDALLQFREFIDENSGEFFVQVWG
                     NGATFDNVILRRSYERQGIPCPWRYTNDRDVRTMVALGLVMDFDARTTIPFEGER"
     gene            complement(2480..2668)
                     /locus_tag="ACHOUK_004567"
     CDS             complement(2480..2668)
                     /locus_tag="ACHOUK_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFP9899863.1"
                     /translation="MTSAFALVMTVFLITGESQNVITGIYASKESCLQARDEQKISGE
                     CLPVKKVSLYLNNETPAG"
     gene            complement(2665..2853)
                     /gene="dicB"
                     /locus_tag="ACHOUK_004568"
     CDS             complement(2665..2853)
                     /gene="dicB"
                     /locus_tag="ACHOUK_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFP9899864.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNKAFTEDAINMRKYEPYLLNDN
                     SILSRIALIKLGIFGERQ"
     gene            complement(3334..3486)
                     /locus_tag="ACHOUK_004569"
     CDS             complement(3334..3486)
                     /locus_tag="ACHOUK_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFP9899865.1"
                     /translation="MDMLNLGNNESLVCGVFPNHDGTFTAMTYTRSKTFKTEAGAHRW
                     LARNAN"
     gene            complement(3761..4405)
                     /locus_tag="ACHOUK_004570"
     CDS             complement(3761..4405)
                     /locus_tag="ACHOUK_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFP9899866.1"
                     /translation="MESLGFRLKRLRKDKGLTQVELGKLSGVTGVTIGYWEKDLNEPG
                     SKALSKLAQALGTTESYLLYGVSSPELSFVQSTSGTKIPYLSWGEAISFLILKGEKTM
                     GNVDRITTFFDVEEGDFAVSMPDDTMHNPSGSPSIPVGATVILRPGESYKNGSIVAVI
                     VPDPLKNEPSMTIKKLVIDGKLVYLSPLNPRYQSSLLTPECKIVAVAKGVQFNL"
     gene            4503..4730
                     /locus_tag="ACHOUK_004571"
     CDS             4503..4730
                     /locus_tag="ACHOUK_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFP9899867.1"
                     /translation="MRVDELVQFFGSVQRVADFYGITREAIYMWRKRPGEIVPKGRAA
                     EAAAYSKGKLSLNPELYKKKDTTQGEGKGDS"
     gene            4727..5152
                     /locus_tag="ACHOUK_004572"
     CDS             4727..5152
                     /locus_tag="ACHOUK_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFP9899868.1"
                     /translation="MKIKHEHIRMAINAWAYPDGEKVPAAEIARTYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDAVEKIQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVAAAIAWGTLTNSGGQPGNAVVVH"
     gene            5221..>5874
                     /locus_tag="ACHOUK_004573"
     CDS             5221..>5874
                     /locus_tag="ACHOUK_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HFP9899869.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTTCHGVSLRGHIDVTTEDLASALDVTEDVIDSILHAMQGRVLDGDLISGWEKRQV
                     MKEDNGNVSQTAKSPAERKRAQRERERLRKQNTDCHDESRRVTHMSRQITTDTDTDKE
                     LNPTHNARMRESATTGELNDASLQTAEPEYLDGLSEPIGKFPMTGVWQPSPDFRQRAA
                     "
BASE COUNT         1417 a         1394 c         1412 g         1651 t
ORIGIN      
        1 cgttcacctt cgaatggaat agtcgttctt gcgtcgaaat ccatcaccag tcccagagca
       61 accatcgttc ttacatcgcg atcattggtg taacgccacg ggcaggggat cccctgccgt
      121 tcatatgaac ggcgtaaaat cacgttgtcg aaagttgcac cgttacccca gacctgaaca
      181 aaaaattcac cggagttttc gtcgataaat tcccggaatt gcagcagtgc atcatccaac
      241 gtgatttcat cggtcagaat ggaggattgt gcttcgcgtg actgtttcag ccaccactta
      301 atggtgtccc gatcgatgac cccacctgcg gtttccagat cgatagtttt gctgaattct
      361 ggccccatct ctccggttgc cggatcaaaa aacttaccgg ctatagagtt tattggcgca
      421 tcaggatttt ttcccattgt ttcaaggtca atcatcagat ggtgccacaa cctgctggtg
      481 gatgagattt catgatgacc gttcacttta attaagggat ttgctgtttc gccagtttta
      541 ttatcgctgg cgtgatactg atcgctgcca gtgttctcct tgtgcagatg ttcagtgcct
      601 tccatttcct ccggatcatt tttctgagct tcagcctgat tctcttcatc gaatgtttcc
      661 aggtaggttg cgttccccat caccgcacca cagtcaggac agttgccgcc accggtctga
      721 ccgcatgcgg tgcaaacttt ttccagttcc tgttgcactg ctggttctgg ttgttgttct
      781 tctggcccgt tttgttgcgt atccgggctg ttttgttccg cttctggctc attttgtttc
      841 acgttgggct gattctggtc ctcagcgtcg cgactctgga tccctttcac ccatttcgga
      901 tcattcgggt cgctaatccc ttcaacaaat tcaccacgtg atgcagcaag caatttatcg
      961 gcgtcaggct ggctgatatt ggctgcctgc ataattttgt ttacttcgtc agcggtaact
     1021 tttaccggct ctgattgttc ggaatcttca gcggtatcca cattttgcgg taagcccgtg
     1081 tatgtgccat tttttcgggc aaaatattct tcttttgaga tttcggtacc gccggcagcc
     1141 agcgccttgt ccagaccaga aagtttgttt gccctgccgt atttttcccc gcccttatcg
     1201 gtaaagacga aataaaatgg tccttcacgc tctacagatg gttcagcttc caccaggatt
     1261 tcattttttt gagtatcgga ttctgccgtc tccactggag cagtttgtgc tgctgacggc
     1321 tggagagcat cagtagagct ctggtctgtt tcttcatgtt caaacacgcc ctttgtcgtc
     1381 aggtattcac tgatatattt gttcagtgca acgggatctt tgtgaatatc gatcggacgt
     1441 tcgcgaacaa ggccaaaaat agtctgacgg tcatagccaa gtgcttcggg ctgtttgcgc
     1501 attgatgctg agatacgctt ccagtcttcg cgatccttgt cgataacctc atttgtcgcc
     1561 cagcgatgga tggtaccgtc aatgtttcct gcatccacat caccgggcca gagggcgcag
     1621 gccagttcat tgtcgagtgt tttccatgtc tgtttgtatt cgcggcgaat ggcaacagtg
     1681 acagccccgg ttttttcagc agcgttttca gtgttctgtt ggttgactct ggcgcgggtg
     1741 agatcaacaa cagacgtgta ttttccggtt tccttgcgtt caccttcgcg acgttttttc
     1801 cagatgcgca tctctgcctg aatttcaggc catttagcgc caggctcaca tttatgctta
     1861 acccacccga tggcatgcag tttaagttca ggatacatgg cgttaacttc tggcattttc
     1921 atcaacgctt caacgatatg tccgccgaat gttgccatgt cttcctgtag taattcctgt
     1981 gcgctaatca ccatatcaac ggtgatgttt tcgcatgtgt cgaatttaac caggactgcg
     2041 ttctgtactt caggggacag cttgtcaaaa ttgacgttca tcggatcgga ttctggttcg
     2101 accggtacaa aggaagctga ctcctcatcc cagcggtttt cctgcatata ttcggtatcc
     2161 caggaatcga gggcagggcg gggtatgccg ggtttatcct cgcagacaat aaatttataa
     2221 gcgcagtcct gagctgccgg gaattgctcc agaaattgcc agtgaaattt tgcgcgtgcg
     2281 cgacgctcat caccggcttc aatggcagtg gccaccgcaa cagcgctatc ttcttttatg
     2341 gcctgttcgt caggaatagc agcgcaaata aagattttac tcattgtgtt ttaacctcat
     2401 tacagatttc agggtgaacg aatccctgcc attgctggca tttttaatcc gttggtatgg
     2461 cgttaatatg gctggcgggt tatccagccg gtgtttcgtt attcaggtac agcgatactt
     2521 tttttaccgg taggcattca ccagaaattt tttgctcgtc tcttgcctgg aggcaggatt
     2581 ctttactggc ataaattccg gtaataacat tctgtgattc acccgttata agaaaaaccg
     2641 tcatcaccag tgcaaatgct gaagtcattg acgttctccg aaaataccaa gtttaataag
     2701 ggcaattcgg gaaagaatgg aattatcatt gagcagataa ggctcatatt tccgcatatt
     2761 aatggcatcc tcagtaaacg ctttattact gagcagaaca ccaatatcaa aacaaccttc
     2821 agacgtatta acgtttggta ataacgtttc cattatcgcg tcctcaacaa tgaattttgt
     2881 gatgcagtgc ctggtgcctc caggtgacgt taaccagtta acaattaacg tcgggttagt
     2941 tgatgctcgt tacgccagta aaagaccgcc ttactgcttt aactgttccg cgtgcgcata
     3001 gccgcattca ccgcatcaca aaattcactt taaaaagggc ggatatccat ttccgccgaa
     3061 tcaccagaaa agtgataaca gaggttgttg tggctggggt gtcacttaag cgtatggtca
     3121 acctgacaac ccggtgtcct caacggggga aggaataacc ccgccatact taccgccgcg
     3181 ccatttcgcg gattgccaca accagaagcg cacggtcgaa ttaaatttaa cgacgaccta
     3241 tacagagaga ctaacttcgc cgtgcgcttt cgtgttatgc cctgactttt cagggtaaat
     3301 taacctgtgg aaacctgttt ttactggcgc taatcagtta gcgtttcttg ctaaccagcg
     3361 atgcgcgcca gcttcggttt taaacgtttt acttctggta tacgtcatcg cggtaaacgt
     3421 gccgtcgtgg ttggggaata ctccgcacac cagagattca ttgttgccaa gattgagcat
     3481 atccatgttg acctcattta cccttaacgc cgggtcgcgg aactaaaaac ctgctgcgct
     3541 gttatacaaa gtgttcccgc cgtcatgttc atacgcctcg ggctggctac ttaacccctg
     3601 accactgccg ggtaactcga ggtattgccc gacgttctgt ggggcgaggt agggtggtat
     3661 gtaataaatc taaaagtatt tagtttttca gtcaaggtaa atctagatta atttaaaaaa
     3721 aaggtcggca ttgccgacct caagtaaagg acgtggcggg ttataagttg aactgtacac
     3781 cttttgctac ggcaacaatt ttacactctg gtgtaagtaa ggatgactga tagcgtggat
     3841 tgagagggct taaatacaca agcttcccat caataactaa tttctttata gtcatagatg
     3901 gttcattttt aagcggatcc ggaactatta cagcgacgat gctgccattt ttataacttt
     3961 ctcctggcct taggatcaca gtagcaccaa ctgggatact cggtgatcct gatgggttat
     4021 gcattgtgtc atcaggcatt gaaacggcaa aatcaccttc ctcgacatca aagaatgtgg
     4081 tgattctatc gacatttccc attgttttct ctccttttaa gattaggaaa gaaatcgcct
     4141 caccccacga aaggtagggg atcttggtgc ctgaggtact ttgtacaaaa gataattcag
     4201 gagacgatac tccatatagg agataggact cagtagttcc taatgcctgg gctaacttac
     4261 ttagagcttt gctacctggt tcgtttagat ctttctccca gtaccctata gtaaccccag
     4321 tcacgcctga aagcttaccc agttctactt gggtcagccc cttatctttt ctaagtctct
     4381 taagcctgaa gccaaggctt tccatcattt tctcctgcaa attgaatata aattatttta
     4441 gattgcattg acctaaaaaa aattatcttg taatctaaaa atgcttagat ttaggaggta
     4501 aaatgcgagt tgatgaactt gtccagtttt ttggttctgt tcagagggtt gccgattttt
     4561 atgggataac ccgcgaagct atttacatgt ggcgtaagcg tcctggtgaa atagttccga
     4621 aggggagggc tgcggaagct gctgcatact ccaaggggaa attatctttg aacccagaac
     4681 tttacaaaaa gaaggatacc actcagggcg aaggaaaggg ggattcatga aaatcaaaca
     4741 tgagcacatc cgcatggcga tcaatgcctg ggcctatccg gacggtgaga aagttcctgc
     4801 agctgaaata gcccggactt atttcgagct ggggatgacg ttcccggaac tgtacgacga
     4861 cagccatccg gaagccctgg ctcgtaatac ccagaaaatt ttccgttggc tggataaaga
     4921 cacccctgat gctgttgaaa aaattcaggc tctgttaccg gcgatcgaaa aggcaatgcc
     4981 gcctctgctg gtggcccgta tgcgcagtca cagctctgaa tattaccgtg agatcgtcga
     5041 acggagggat cggctggtga aagatgtgga tgattttgtc gcagcggcga tcgcctgggg
     5101 caccctgact aacagtggtg gtcagcctgg taatgctgtt gtcgtgcatt gaccaacaat
     5161 attcatgccg gatttcttcc ggatgttcga gggtaaagtt cggtatcaga tgaggtgagt
     5221 atggctaatg cctggctcag attgtggcat gacatgccaa atgatcccaa atggcgaacc
     5281 attgccaggg tctcaggaca gccaatcgca acagtgatgg cggtgtatat ccaccttctg
     5341 gtgagtgcgt cacgaaatgt cacgacatgt cacggcgtgt cactacgtgg tcacattgat
     5401 gtcacgacgg aagatttagc aagtgcgctt gatgtgacgg aagacgtgat tgattcaatt
     5461 ttgcatgcaa tgcaggggcg agttctggat ggtgatctta tttccggatg ggaaaaacgc
     5521 caggtgatga aggaggataa cggtaatgtt tcgcaaaccg caaaatcccc ggcagagcgc
     5581 aagagagcgc agcgggagcg ggaaagactg cggaagcaga acactgattg tcacgatgag
     5641 tcacgacgcg tcacgcatat gtcacgacaa atcacgacag atacagatac agataaagaa
     5701 ttaaacccca cacataacgc gcgcatgcgc gagagtgcta cgaccggtga gttgaatgac
     5761 gcgtcgttgc agacagccga acctgaatac ctggacggcc tgagcgaacc gatcgggaaa
     5821 tttccgatga ctggagtctg gcaaccgtcg ccggattttc gacagcgggc agca
//
LOCUS       DAZKIP010000205         5833 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZKIP010000205 DAZKIP010000000
VERSION     DAZKIP010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5833
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.207"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..2390)
                     /locus_tag="ACHOUK_004574"
     CDS             complement(<1..2390)
                     /locus_tag="ACHOUK_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9899870.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELNSQLTEIRGSAVGMAGAFAGAFASGHLISLADEWSSVNARLKQAS
                     QSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSAGDVLKVTEAISTG
                     LKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIVRALAAGMGVARKDLK
                     AMADDGKLTADKVVPALISQLGILRDEYAAMPETVSSSITKVENAFMAWVGGANEASG
                     VTKTLSGMLNGVAGQIDNVATAVGALVAVGVARYFGNMASGAMSATAGLVTAARNEVA
                     LAEAQFRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNRNIAART
                     AAQNALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALT
                     IDEIAHKTPSMSLPEASDNEGRTRAALTEQNRLIDEQASRVKSLQEKIAGYQYVLANP
                     GWTTGDGFMINHLTSVKTVTEGLAQATEQLAVEQSRLAQMQEKAQSIQDVLAGLEDRR
                     VALIRQQAAEQNKVYQSMLVMNGQYTEFNRLLGLGNELLQQRQGLVNVPLRLPQATLD
                     DKQQSALTKTERELALSRLKGEEKERVRLGYAADDLGFVGDPYQEARQRYISNALEAW
                     RNNEANKPKSRGGKSETEKAEDSFSRLLKQQKEQLALAGQNTELAKLKYQTALGELKT
                     LSEIQKQELLRNAALIDQQKIREQLRYREETLKNDNVAARASNESELLGYGQGERA"
     gene            complement(2443..2652)
                     /locus_tag="ACHOUK_004575"
     CDS             complement(2443..2652)
                     /locus_tag="ACHOUK_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFP9899871.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEVDPFAALEDALSLAAMS"
     gene            complement(2748..3122)
                     /locus_tag="ACHOUK_004576"
     CDS             complement(2748..3122)
                     /locus_tag="ACHOUK_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFP9899872.1"
                     /translation="MAKDLKTLALARLSGFRHKTVKVPEWRNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            complement(3128..3844)
                     /locus_tag="ACHOUK_004577"
     CDS             complement(3128..3844)
                     /locus_tag="ACHOUK_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFP9899873.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMEKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDTVYAFKVQ
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSYVVPLAFVKNLEKTLTVN
                     TGALLTMSVSVNGGTPPYKHAWKKDGQPVEGQTTDTFSKANTQSGDKGAYTCEVTDSA
                     EQPQSITSDACTVTVNGAGG"
     gene            complement(3903..4247)
                     /locus_tag="ACHOUK_004578"
     CDS             complement(3903..4247)
                     /locus_tag="ACHOUK_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFP9899874.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVSVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            complement(4244..4690)
                     /locus_tag="ACHOUK_004579"
     CDS             complement(4244..4690)
                     /locus_tag="ACHOUK_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFP9899875.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRCSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            complement(4687..5037)
                     /locus_tag="ACHOUK_004580"
     CDS             complement(4687..5037)
                     /locus_tag="ACHOUK_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFP9899876.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDHVLNVVGVPVPDAT
                     GGRLEILCRLGGEK"
     gene            complement(5047..5373)
                     /locus_tag="ACHOUK_004581"
     CDS             complement(5047..5373)
                     /locus_tag="ACHOUK_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFP9899877.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDEMLMDKVRQATAVLLAYIQGSR
                     DKVISEDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREDLVQGELPFSVSVLIYDL
                     RCPTVL"
     gene            complement(5370..5833)
                     /locus_tag="ACHOUK_004582"
                     /pseudo
     CDS             complement(5370..5833)
                     /locus_tag="ACHOUK_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
BASE COUNT         1189 a         1939 c         1383 g         1322 t
ORIGIN      
        1 ctggctcgtt ccccctgccc gtaccccagc agttcagatt catttgatgc acgcgcagcc
       61 acattatcat tcttcagggt ctcttcccgg taccgcaact gctcccggat tttttgctgg
      121 tcaatcaggg ccgcattgcg cagcagttcc tgcttctgta tctccgacag ggttttcagt
      181 tcacccagcg ctgtctggta cttcagcttc gccagctccg tgttctgacc ggccagtgcc
      241 agttgctctt tctgctgctt cagcagccgg gaaaaactgt cttccgcttt ttccgtctct
      301 gattttccac cccgggattt gggtttattc gcctcgttat tgcgccaggc ttccagggca
      361 ttactgatat aacgttgtct cgcctcctga tacggatccc ccacaaaacc gaggtcatcc
      421 gccgcatacc ccagtcggac gcgctctttt tcttcccctt tcagtctgga cagggccagc
      481 tcacgctctg tttttgtcag ggcactctgc tgtttatcat ccagagtggc ctgtggcagc
      541 cgtaacggca cattcaccag tccctgccgc tgctgaagca gttcattccc cagccccagc
      601 agacggttga attccgtata ctgaccgttc ataaccagca tggactggta caccttattc
      661 tgctctgccg cctgctgacg aattaacgcc acacgacggt cttccagccc ggcaagcaca
      721 tcctgaatgg actgcgcttt ttcctgcatc tgtgccagac gggactgctc aacggcaagc
      781 tgctctgttg cctgagcaag cccttccgtt acggtcttca ccgatgtcag atggtttatc
      841 atgaatccgt caccggtcgt ccagcccggg ttcgccagaa catactgata tcctgcgatt
      901 ttttcctgca gggatttcac ccgactggcc tgttcatcaa tcagccggtt ctgctctgtc
      961 agcgccgccc gtgttcgtcc ttcattatct gaggcttcag gcagagacat tgacggcgtt
     1021 ttatgcgcga tttcatctat cgtcagtgca tactggcgcg cagactccct ggcctgctcc
     1081 tgattctggt acagcgtgta ccatgctgca gcccccagca tcaccagtcc gggtacgcca
     1141 ccaaccagcc ccagcgcacc gctcatcaga cgtgagccca ccgccgttgt actgttcagc
     1201 gcattctggg cggcggttct ggcagcaata tttctgttca ggcgttcctg tgtggccgcc
     1261 agacgggcct ctgctgcaat ctgcatctcc gtcccgcggg ctgccgccac ggcctgctga
     1321 gcacggtaca cggctgccct tgcccgcgcc gtggcaatct gcgttcccct gaactgtgct
     1381 tccgccagtg caacttcatt acgtgcagcc gtcacaagtc ctgccgtggc agacatcgct
     1441 ccggaggcca tattgccaaa gtaacgtgca accccgacgg caaccagcgc gcccacggct
     1501 gttgccacat tatcaatctg tccggcaaca ccgttcagca tgccggagag cgtttttgtc
     1561 accccgctgg cctcattcgc accgcccacc caggccataa aggcgttttc caccttcgtg
     1621 atactactgg aaaccgtttc cggcatggcc gcatattcat cacgtaatat ccccagctgg
     1681 ctgattaacg cggggaccac tttatccgct gtcagttttc cgtcatccgc cattgccttc
     1741 agatctttac gggccacgcc catacccgca gccagtgcac gtacgatccg gtcaccactt
     1801 tcattgaccg aattaaattc ctcaccgcgc aacacaccct gtgccagcgc ctggctgaac
     1861 tgggtgatca ccgaacccgc ctcagccgta ctggcaccgg agattttcag ccccgtggaa
     1921 atggcctccg tcaccttcag tacatcaccg gcactgtaac catattcacg catcgaggca
     1981 gccgaacggg caaacagggc cgcattatcc gaaaatgccg tgcctgtccg ctggctgata
     2041 tccatcagca ctttctgtga tgacgaaaat tcatcggatg actgtgatgc ctgtttcaga
     2101 cgggcattta cggaactcca ctcatccgcc agtgaaatca ggtgtccgga ggcaaaggca
     2161 ccggcaaatg cgccagccat tccgacagca gaaccgcgaa tttccgtcaa ctggctgttc
     2221 agttctgcca gggcacgtcg ctgctcccgg gctgccgcag cggcctgacg cccgccattc
     2281 tgcagggtcc ggtaatattc actgcccata cgggacgccc gctggatctc cgactggaat
     2341 gactgtgaat ttgccgaaat tttgataatc agttcacgta acgtcgccat tcacctttct
     2401 ccgggcaaaa aaaaacctgc cacagcaggt tttcatcatt atttatgaca ttgctgcaag
     2461 gctcagcgcg tcttccagcg ccgcaaacgg atccacctcc ggcttatcct catcctcgcc
     2521 ccagcagagc atggcgtcct tcagtgcaac attcatcccc tgtgccccga aaaccgcttt
     2581 cacgatctgt gcattacgga tatccccgcg ctcatcaccc agcggggata ccctgtcgaa
     2641 ctccatccac atcatcgcct cgctcgcact caggctgtgc cgcagttcgg ataaggtgcg
     2701 ccccagacgg agcgcaagtc gcatcagaaa gcgaatttcc gggcgggcta cttttttctg
     2761 gccgactctg catcagcgat cagttccagt gcctgacgca gcaaccgggc atgtaccgga
     2821 ccatagacgg ccagcacctg ctcacggtcg tccggagtga acacccgttg caggtcagta
     2881 tcacacagga catcgcagaa cagcgtcaca tccgcttcca ggttacggcg ggttttcgcc
     2941 accaccgaca gggtatcgtc atcctctcca tcaccattga gcacttcctg ccacagatac
     3001 caggcctctg ccgaaggctc ccgcagcacc acgctgacat ttctccattc cggcaccttc
     3061 accgttttat gacggaaccc cgacagtctg gccagcgcca gtgttttcag atcttttgcc
     3121 ataagcctta tccgcccgca ccattaaccg ttactgtaca cgcatcagag gtaatgctct
     3181 gcggctgttc tgcagaatcc gttacctcgc aggtataagc ccccttatca cctgactgcg
     3241 tattggcttt actgaaagtg tcagtagtct gtccctctac cggctgacca tccttcttcc
     3301 aggcgtgttt ataaggcggc gttcccccgt tgacactgac tgacattgtc agcagcgcac
     3361 cggtattcac ggtaagtgtc ttctccagat ttttcacaaa cgccagcggt accacatagg
     3421 acaccggttt acccttcagg cgaagtgaaa acgttgcagc caccacgccg ttggtaccgg
     3481 atgaccaggt gtgctgacgc acttccgcca ggaacttaaa gcccttaccg gacggaaact
     3541 gcaccttaaa cgcatacacc gtgtcattgt cataggcatc acgcagggcg ttctgggcct
     3601 gattcagata aaaattaccc gacatggaaa tctcggacga cgcccccaga ccgttgatgt
     3661 tctcctgctc tgtggagcag agcgtggtca catcaatatc ctgtttctga ccggcggtga
     3721 actggacttc cttgatggtg cagtccaggc gcagatattc cgccttctcc atggtttcag
     3781 cagtcgccgg ggcagatgaa atcatcacct gcgtcagctg tgaacgttca tacaaagcag
     3841 acattctgcc tcctgataat aaaaaacccg cacgcggcgg gttatgggtt ctgttgaaaa
     3901 aattacaccg tgacctgaaa ctccagggtt gcacggtaac agcggttttc cggaatatag
     3961 tcctgcattt cactgacgga tcccggggcc agcagcatta tggcttcacg ggcgtcctga
     4021 cgtatctgac gcgcctgcgt cacagtcccg gcataaacgt ctatctgcac cgacactgag
     4081 gactccgcct gcccgcccat cacgtccgcc gacaccgatg aaatcaggct gaaaaccacc
     4141 cacggaagcg ccaccgacgg cctgccatcc agcaggggga ccacatacgg gtacacctgc
     4201 ccgccggcaa gatgcgccag atgaggatac aaatccgcct ccgtcatcgt ctcagtacct
     4261 catcaatggc ccggttcatc cgagcaatcg ccacctgtgc cgcctgttca ctgcgcacat
     4321 caaatgccgg gcgcacaaac gggtgcggtg gcatattcac ggtccccatt tccacaaacc
     4381 gccagtagaa agcattgcgc gggttatccg ccttcatggt gttatcgctg ttaccggtgt
     4441 ccggattaac accccggata tgcacaccgg attccatccc gccatcgcgg gagcaccggg
     4501 aaaggaccac cacattgcgg cgaagttttc ccctgcgtac cggtgcccgt gacaccactt
     4561 cttctttcag cacattcgca cccgcacggg ttgcctcacg cagcacccgg ttattttccg
     4621 caccactcag aagctgcaaa tcgcggctga tgtcctccag ccccgaaaaa tccagcaggg
     4681 tttcgatcat ttttcccctc ccagccgaca gagaatttcc agacgcccgc cggttgcatc
     4741 cggaaccggg accccgacga cattcaggac atggtcacgc cagggaccac tcagcacatg
     4801 aagtcgtgac gccgccgtga tttcacgacc agactgaccg cgtacccaga tgcggatttc
     4861 cgcctgcgcc atttccgcac cggactgcat ccgctcccgg ctgctcctgc cccggatatc
     4921 cgcatgaatt ttcccgcatg acacccattc ttccgtcatt tctccggcag cattacgggt
     4981 taacaccggg ttcagaacac tgatcatctg tgtcagacga cctgcagata ttgccattcc
     5041 ccctcctcat aacaccgtcg gacaacgcaa atcgtaaatc agcacggaaa cagaaaacgg
     5101 cagttccccc tgcacgaggt cttcccgctc agcaagatcc ggattccggt acagcatccc
     5161 ggtcagtcgc atggcagccc ccttcatccg ggttaatgcc tcaccaggga tcagctcacc
     5221 gtcctcacta atcactttat cccggctgcc ctggatgtag gccagcagca cggcggtagc
     5281 ctgacgaacc ttgtccatca gcatctcatc atccgcgtca tggtcaacac gcagatgtgc
     5341 cttgatttct tccagtgtca gtaatgctgt cactttccac ctcctgcatc ccgcccacgt
     5401 tttgcagcca gagtccagcc tgatgaatga gcttctccgg gcttatcacc ggtcatactg
     5461 ttgcagtgcc acaacgagcc ccccacgtca ccgtatcgcc ggggtggtaa gtttcgccga
     5521 ttctgaacac accacggtag agcatcaccg gcagggaaaa tgttttttcc gtacgctggc
     5581 cactgctctg ccggaccacc acagagaacg accgttcacc cgtcatgctg acgtcaatat
     5641 ccgccacccc gtcaaccagg cattcccatc cccgcatccc gtacgttttt tcatacgccc
     5701 gccagagtcc accctggtgt gtggcatacg agccccgggg aaaggatttt tgatcgtcaa
     5761 tggcggggag tatttccagt gccgtggcat cacgcccgtc ctgcggagcc ggcagggcac
     5821 tcaccgcctc cag
//
LOCUS       DAZKIP010000206         5509 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZKIP010000206 DAZKIP010000000
VERSION     DAZKIP010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.208"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..1426)
                     /locus_tag="ACHOUK_004583"
     CDS             complement(<1..1426)
                     /locus_tag="ACHOUK_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9899878.1"
                     /translation="MWNLLRRTRKNQKSGRDVREAGWTSLFQAVAEPFSGAWQQGVKA
                     DPEAVLSFHAVFACISLISQDIAKMRLRLMQTDAHGIRRETRRGDIARLCRRPNAQQN
                     RIQFFELWLNAKLRHGNTVVLKIRNARGQIKELRILDWSRVEPLVADDGEVFYRITPD
                     RNCGITEAVTVPAREVIHDRFNCFFHPLIGLPPVYAAGLAATQGHHIQENSTSFFRNG
                     GRPSGVIEIPGSITEENAKKLKSNWDSGYTGENAGKTAILSNGAKYNPTTFSPVDAQT
                     VEQLKMTAEIVCSVFRVPAYKIGVGQPPSSDNVEALEQQYYSQCLQTLIESIELLLDE
                     ALETGENESTEFDVTTLLRMDSERRMKTLGDAVKNTLLTPNEARKRENLPPLAGGDAL
                     YLQQQNYSLEALSRRDAREDPFASAGKTVSSQLPDGASDGNKAISETEHDAVKAMFRG
                     DTEKMTERELSIIRALGEEFSTVLA"
     gene            complement(1372..1593)
                     /locus_tag="ACHOUK_004584"
     CDS             complement(1372..1593)
                     /locus_tag="ACHOUK_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899879.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFYGEPEKTRNQDVT"
     gene            complement(1638..3575)
                     /locus_tag="ACHOUK_004585"
     CDS             complement(1638..3575)
                     /locus_tag="ACHOUK_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFP9899880.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSAIPFLWQHDHSRPVGQCTVRRVSEGLEITATLVKPVPDMPSQLAARLDEVWAAIK
                     TGLVRGLSVGFRPHEYTFLDGGGLHFLRWELMEVSAVTVPANAECTIRTIKSYDRPFS
                     AASGNRKPVVKIASSAGAAAQSTTVFHKEKTIMNIGEQIKSFENKRAALAASLEEVMT
                     KAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELEAGKAATAQPVKQAGNGNVAA
                     VASAPVIRVEQKLDKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAV
                     GAGTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRVHAQV
                     SGGAAGWVGEGKAKPLTKFDFESITFSHAKVSAIAVLTEELIRFSSPAADALVRNALA
                     EAVVARLDTDFVDPKKAAVADVSPASITHDVKGTASTGNPDADAEAAFGQFVAANLQP
                     TGAVWLMSSTNALALSMRKNALGQKEYPDMTLLGGSFQGLPVIVSQYVGDQLVLVNAP
                     DIYLADDGGVAVDMSREASLEMQSEPGGDSTTPSPVELVSMFQTGSVAIRAERWINWR
                     RRRTAAVAVITGVNYGSASGG"
     gene            complement(3639..5300)
                     /locus_tag="ACHOUK_004586"
     CDS             complement(3639..5300)
                     /locus_tag="ACHOUK_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFP9899881.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPEQAAHALAIFKQLRI
                     VDAPGSPTFGESCAQWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGAHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWAGADFWEQ
                     QGRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVV
                     RRKSEASRFQDLVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQIL
                     DSLAEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGDQPLMAWCVGNARVEPK
                     GNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPKKKEYAVFFI"
     gene            complement(5297..>5509)
                     /locus_tag="ACHOUK_004587"
     CDS             complement(5297..>5509)
                     /locus_tag="ACHOUK_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899882.1"
                     /translation="AMGRLLVENINTDPRLALDAAYKLAQFTHHKKGDAGKKSAKGDA
                     AKKAANRFAVPPPPRLVVNNDNEGNG"
BASE COUNT         1119 a         1796 c         1402 g         1192 t
ORIGIN      
        1 ccgccagcac tgtggaaaat tcttctccca gtgcacgaat aatggacagt tcccgttccg
       61 tcattttctc agtatccccc ctgaacatcg ctttcaccgc atcatgctct gtttcactga
      121 ttgccttatt accgtcagat gcgccgtcag gcagctgtga tgaaactgtt ttcccggcag
      181 acgcgaacgg atcctcacgg gcatcacgac gggacagcgc ctccagactg tagttctgct
      241 gctgaagata cagtgcatca ccgccggcca ggggcggcag gttctcccgt ttacgggcct
      301 cattgggcgt gagaagcgta tttttcaccg catcccccag cgttttcatg cgccgctcac
      361 tgtccattct cagcagcgtg gtgacatcaa attctgtact ctcgttttcc cccgtttcca
      421 gcgcctcatc cagtaacagt tcaatggact caatcagcgt ctgcaggcac tgggaataat
      481 actgctgctc cagcgcctcc acgttgtcac tggaaggcgg ttgtcccacg ccaatcttgt
      541 aggccgggac acggaacacc gaacagacaa tttcagcggt catcttcagt tgttccaccg
      601 tctgcgcatc caccggtgaa aacgtcgtgg ggttgtattt tgccccgttg ctcagaatgg
      661 ccgttttccc cgcattttcg cctgtatacc cgctgtccca gttgctcttc agttttttcg
      721 cattttcttc cgtaatactg ccggggatct caatcacccc ggacggcctg ccgccatttc
      781 tgaaaaaaga cgtcgaattt tcctgaatat gatgcccctg cgtggccgcc agcccggcgg
      841 catacaccgg cggcaatcct ataagcggat gaaaaaaaca gttaaaccgg tcgtggatca
      901 cttcccgggc aggcaccgtc accgcctccg tgatcccgca gttccggtcc ggcgtgatgc
      961 ggtagaacac ctcgccgtca tccgccacca gaggttcaac ccggctccag tccagaatac
     1021 gcagttcttt gatctgcccc cgggcattac ggattttcag caccaccgta ttgccatgac
     1081 gcagtttggc gttcagccac agttcaaaaa actggatgcg gttctgctgg gcgttgggac
     1141 gacgacagag gcgggcaata tccccccggc gcgtttccct gcgtatccca tgcgcatccg
     1201 tctgcataag acgcagccgc attttggcga tatcctggga tatcagcgaa atacatgcaa
     1261 acaccgcatg aaaggagagg acggcttcag gatcggcttt cacgccctgc tgccaggcgc
     1321 cggaaaaggg ctcagccacc gcctgaaaca ggctggtcca gcccgcctct cttacgtcac
     1381 gtcctgattt ctggtttttt cgggttcgcc gtaaaaggtt ccacattcgc catgctccgc
     1441 atcacgtttc tttttctgac ctgccggacg tcgcaccgtg atgtactccg cctttcccag
     1501 gcgaaccagc acctccgcac acggctgtgc cacatcacgg atatccccgg cccgggcatc
     1561 atgcgtgccc tgcagatatc ggatctttgc cataacctgt tacgggaggc gcacgcctcc
     1621 cgtcctcctt atcagactca gccgccggac gcactgccgt agttcactcc ggtgatcacc
     1681 gccaccgccg cagtacggcg acgacgccag ttgatccagc gctccgcacg gatggccacg
     1741 ctgcctgtct ggaacatgga aaccagctcc accggggacg gcgtggtact gtcgccgccc
     1801 ggctcagact gcatttccag tgatgcctcg cgggacatat ccactgccac gccgccgtca
     1861 tccgccagat aaatatccgg ggcattcacc agcaccagct ggtcacccac gtactgggag
     1921 acaatcaccg gcagcccctg gaaggagcca cccagcaggg tcatgtccgg gtattccttc
     1981 tgacccagcg catttttacg catggacagt gccagggcat tggtgctgga catcagccag
     2041 accgcaccgg tgggctgcag gtttgctgcc acaaactgtc caaacgcagc ctcggcatcc
     2101 gcatccgggt taccggttga tgccgtgccc ttcacatcat gggtgatgga cgccggggag
     2161 acatctgcca ctgcggcttt tttcgggtcc acaaagtctg tatccagacg cgccaccacc
     2221 gcttccgcca gcgcattacg gaccagtgca tcagcagccg gactggaaaa acggatcaat
     2281 tcttccgtca gtaccgcaat ggccgacacc ttcgcatgac tgaaggtgat ggattcaaaa
     2341 tcaaacttcg tcaggggttt tgccttaccc tcacccaccc agccggcagc accgccggac
     2401 acctgggcgt gcacacggat attgaatggc acctgacgaa gtgcagggat cccgccctga
     2461 ccaaatcgcc cgataatggt ctgcggacgc aggtaatcaa taaagtcctg tgcgtattcc
     2521 tgatattcag acaggctgcc tgcccactgc ggatccgtgg tggtccccgc gcccactgcc
     2581 gatttcagga catgatgcag acgactgtca tccggatact gacgacgggc cacttccagg
     2641 gcttcagatc ggacgccttt agccgcagcc agcgatttgg caaagcgggc gaagccaatc
     2701 cccttatcca gtttctgctc cacacggatc accggcgcag aagccaccgc ggccacattc
     2761 ccgttaccgg cctgtttcac cggctgcgcc gtggcggcct taccggcttc cagttcacgc
     2821 aggcgcttca ggtgcgcatc cacctgacgg atttccgctg cggtgttgtc gtaatgctct
     2881 tcctcctcca catccagcgt gcgcccttcc tctgcggctt tggtcatgac ctcctcaagg
     2941 gaggctgcca gcgctgcacg cttgttttca aaacttttaa tctgttcgcc aatattcatt
     3001 atggtctttt ccttatgaaa aacggttgtt gactgtgccg cagcgccggc agaagatgcg
     3061 attttcacca ccggtttccg gttgccggac gcggcagaaa acgggcggtc gtaagattta
     3121 atggtccgga tggtgcattc cgcattcgcg ggcacggtga cggcagacac ctccatcagt
     3181 tcccagcgca gaaaatgcag tccgcctccg tccagaaagg tgtattcatg gggacggaag
     3241 cccacggaca gccccctgac cagcccggtc ttaatggccg cccagacctc atccagccgg
     3301 gcagccagtt gcgacggcat atccggtacg ggcttcacca gtgttgccgt gatttccagc
     3361 ccttcgctga cccggcgcac cgtacactgc cccaccgggc gggaatggtc atgctgccag
     3421 agaaacggga tcgcactgcc aaactccgcg ccctccggct ccaggatgtc accatcccga
     3481 tccggagaag gcgttgacgc aatcccggtg atcacccgtt catcctcact gaaggatttc
     3541 accgtcagca gggaacaggc ccgtttaaga gtcacatcag cctcctgaaa ataaaaaaac
     3601 cgccgcagcg gttcatgatg gttacagtgt gaacagggtt atatgaaaaa aaccgcatat
     3661 tctttctttt tcggttccgg gttaagggac atcagggaga ccgcattgaa cagcgccatc
     3721 agcgggtcaa tttttccccg tccactggcc tgtttggtga taagaatggc gttaccttta
     3781 ggctccaccc gggcattgcc gacacaccag gccatcaggg gctggtcacc atgcaccagc
     3841 accccttcag ccagtttgcg ctcggtggtt ttaatggccc cgcccagttt ccagccctgg
     3901 cttatcccca ccacaattcc gtcggggatc ccggcttccg ccagtgaatc cagaatctgc
     3961 cccacccctg acgggtcaat accgatatgg tccagtaact cagcctcatg aatgcgacgc
     4021 acatattccg ccacttccgc cgtgtcatcc ccgacacgcc ggacaatggt catatctcca
     4081 caggcaacaa gatcctgaaa ccgggacgcc tcgctcttcc gtcggaccac cgcggtttca
     4141 tgcgcccagg catggcccca gcccagccat tcgcgggttt ccctgtcacg gccaatcacg
     4201 tacattccca gcagatcatc caggcccccg ccgtcaatcc ccaccgtcac cacatcagcg
     4261 cgctgcagga tatcgtccag gctgacgcgc ctgccctgct gctcccagaa atccgcaccc
     4321 gcccagcggt cagaacgcag ggcaagacca atttccacat tggcatgttt tgacatgaag
     4381 ccacgaaatg cttcctcacc agcctcccgg gctttacggt actcccggta cagaaaagcc
     4441 tcatccaccg aataaccgag attcgggtta accatggcga ggttttccat cagcaggtga
     4501 gccccgcttt ccaccatttc aggaggatgc tcaaaaatca ccggcagaaa gtgcggatca
     4561 tgaattttgc cgtcacggac atcccgggcg tactgcagtt tctgtctgaa caccccggct
     4621 ggcggctcat tcgactgggt ggtcgtatac accacaaacc cttccgggcg ggaggcaagg
     4681 ccgcctatgg cttcacgtaa catgtcctcc gctttgtact gcttgccaaa taaccacagt
     4741 tcatcaatca gcgtccccac ggacttgata ccggataccg tattcggatc ggcagccacc
     4801 accttcaggg tggtgtccgt caccctgtgg gtgatggtcc ggatatgtgt ctgcacctga
     4861 cagaggtcat ccagatcatc gtcccgtcgt accatatccc tggcagggtt gaaggcgtta
     4921 gccgccacct ccacggtcgg ggccagaatg gtgtagcccg ccgcctgccg ccagttcagt
     4981 aacagcgccg tcatcatgat cccggcagcc agcgtggact tgctgttttt cttggggata
     5041 aggataaaaa cttccttgat atggcgtaca ccggtctgcg catcgtagga gccaaacagg
     5101 gccgccacca ggtcaaacac ccactgtgcg caggactccc cgaacgtcgg gctgcccggt
     5161 gcatccacaa tccgcagttg tttaaaaatc gccagggcat gtgcggcctg ctccggataa
     5221 atcggagccg gaataatcga cagccccttt ttcaggcgct ctgcccagtc cgggcaggcc
     5281 gtgctccaca caggtatcat ccgttgccct cattatcatt attcaccacc aggcggggtg
     5341 gtggtggcac cgcaaaacgg ttagccgctt ttttcgccgc gtcacctttt gccgattttt
     5401 taccggcatc cccttttttg tggtgcgtga actgcgccag cttataagcc gcatccagcg
     5461 ccagcctggg gtcggtatta atgttctcca ccagaagacg ccccatcgc
//
LOCUS       DAZKIP010000207         5423 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZKIP010000207 DAZKIP010000000
VERSION     DAZKIP010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.209"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..1100
                     /locus_tag="ACHOUK_004588"
     CDS             <1..1100
                     /locus_tag="ACHOUK_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HFP9899883.1"
                     /translation="VQTDDFASSFRLFNVLNSRGLPLSNADLLKNALFESASTHNKKS
                     EQIESAWSQIEDMVGVRRLDKFLTLHKLSEKKDRDRVLQKGFEAFIENLQQQFDGDAI
                     AMSLMLVNSAKNYTKILENDFEHPSIRRKIASLSNLGVDEWIPPVMAFMNRMARTEDF
                     NLDDFSQFITAFEKVYMHGWLKKQIKSQREMVCYSALVAINNDMPFDSVINQINQHAD
                     NSGFIAALDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQALV
                     NEYWINRFQPQEHVYWLHKIGNLTLISGSKNSEAQHYDFIKKKSIYEKLNSKSSFDLT
                     KDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
     gene            complement(1169..2125)
                     /gene="yjiA"
                     /locus_tag="ACHOUK_004589"
     CDS             complement(1169..2125)
                     /gene="yjiA"
                     /locus_tag="ACHOUK_004589"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFP9899884.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(2136..2339)
                     /locus_tag="ACHOUK_004590"
     CDS             complement(2136..2339)
                     /locus_tag="ACHOUK_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFP9899885.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            complement(2389..4539)
                     /gene="btsT"
                     /locus_tag="ACHOUK_004591"
     CDS             complement(2389..4539)
                     /gene="btsT"
                     /locus_tag="ACHOUK_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFP9899886.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            complement(4832..5374)
                     /locus_tag="ACHOUK_004592"
     CDS             complement(4832..5374)
                     /locus_tag="ACHOUK_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899887.1"
                     /translation="MLNRDFDHLSTLLHLFFEREDICQKLNIIDQNNITGWEVWFQVE
                     FANMLCSTDHEWWREQALSCDMRKKPERPTLRTDFLLRKKGWAQDSYIALEIKQNRDA
                     TSCVKNMIADLEKSAKIKRSELDLRSFWTLGITPMIDRERLDSLIDCYLDEKFYLTKS
                     RKKHVLLKEINNTPYCYIVI"
BASE COUNT         1460 a         1273 c         1331 g         1359 t
ORIGIN      
        1 ttgtgcaaac agatgatttc gcatcatcat ttagactctt taatgtatta aatagtcgag
       61 gtttaccttt aagtaatgca gatcttttaa aaaatgcact gtttgaatca gcttctacac
      121 ataataaaaa atcagagcaa atagaatcag catggtcgca aattgaagat atggtcggag
      181 tgagacggct tgataaattt cttactcttc ataagctttc cgagaaaaaa gatcgtgaca
      241 gggtgttaca aaaaggtttt gaagctttta ttgaaaatct ccagcagcaa tttgatggag
      301 atgctattgc tatgtcactg atgttggtta attctgcaaa aaattatact aagattttag
      361 agaatgattt tgaacaccct tcaatacgac gaaaaatagc atcgctaagt aatttagggg
      421 tagatgagtg gattcctccg gttatggcat tcatgaatcg aatggctcgt acagaagatt
      481 ttaatttaga tgatttttct caatttatta ccgcctttga gaaggtatat atgcatgggt
      541 ggttaaaaaa acaaattaaa agccaaagag aaatggtttg ttattcagct ctggtagcaa
      601 taaataatga tatgcctttt gattctgtga taaatcagat aaaccaacat gcagataatt
      661 caggttttat agctgctctg gatgaagatt tatatgagcc tcgtccgaat caagtgaact
      721 taataaaagc aattttgctt cgcttagata tggagcaaca agatgagagc gtgattaaaa
      781 cctatacggg gagaatcaca attgagcata ttcttcctca agcattagtt aatgaatact
      841 ggattaatag attccaacct caagagcatg tctactggct gcataaaata ggtaatttaa
      901 cactcataag tggttccaaa aattctgaag cacaacacta tgattttatc aaaaagaaat
      961 ctatttatga gaaacttaat agtaaaagct cttttgattt aacaaaagat gtttgtaatt
     1021 cttcagaatg gggattggct gaactgaaaa tgagacatga aaaaatgaaa acgcaattga
     1081 agaagctttg gttggtctga catatctaaa gagttccact cgtaataatg ttgcggtgga
     1141 actctcaaaa agttgatctt tcccgaactt acttcctcaa ccccgcaaac gctgcccgaa
     1201 tctcttcctc cggcaactga atcccgataa aaaccattgt gctatgcggt ttttcatcgc
     1261 cccacgggcg gtcccagtcg gcgctgtaga ggcgctggac gccctggaac agcaggcggt
     1321 taggttcgcc gtcaatccac agcatccctt tgtaacgcag cagtttatcc gccgattcca
     1381 gcagcaggtt ttccatcacg cgggaaactt cgctgatatc taccgggtaa tccagttcta
     1441 ccacaatcga cgaaatatcg ttttgtttat ccgcgataaa gtggaaacgc ggtttggtgc
     1501 tgacgacgtt ttcttccagc ataaaaccgt tggtgttgaa cagcaggccc aggtcgatgt
     1561 cgccgtgggt aacggtgtag accggtgcac gggcgttgat gcgcgccagg cgttcgcgca
     1621 gtttttctgc ttcgcctgcg acgtcggttt tggtcagcag aatacggtcg gcgtagccaa
     1681 cctgggactg ggcgatggtg aactggttca tctgctcatc ggcatgtacc gcatccacca
     1741 gcgcaatcac gccgtccagc aggtaacgct ggcataaaat ctcatgggag aaaaaggtct
     1801 gaataatcgg gccgggatcg gccatgccgg tgcattcaat gaccagacga tcgaactgaa
     1861 tattgccctt atcgagatta tccagcaggt cgagtagtgc gtcttccagc tcgttggagc
     1921 gcgaacagca gatgcagccg ttggtcagcg ttttgatctg cgtggcgcgg tcgccaatca
     1981 gttgatcatc aacggagact tcgccaaatt cgttttcaat cacggcaatc ttgtagccgt
     2041 gttgttcgtt aagaatatgg cgcagcaggg tggttttgcc cgcgcctaaa aaaccggtga
     2101 gtagggtaac tgcaatcggg ttcatgctct ctccattaac aacagcgcat accgccttta
     2161 ccatcgccgc cgtagcgcgc gttctggcgt tcgcggaaga attcttcata gctcatgtac
     2221 ggcttgtcgg gatggttggt cttcatatgc tcgacgtagt tgtcgtagtc tggaatgcca
     2281 atcaacatct tcgccgcctg gccgagatat ttttttgcct gtcctaagtt accaaacata
     2341 gttcactctg ataagaacaa agccccgccg aagcggggct aaacacggtt agtggtgcga
     2401 agagatcttc acgccgcctt ccgggattgg aacgtacggt gtttctttgt cagtacgttt
     2461 gtcgctgtta cgtaccgcaa gccaggtttt gaaaccgtag aagatgatgc tgtacaccac
     2521 aatcaggaac agaatactca ggcctgcgtt ggtgtagttg ttcacaacga tgtggttcat
     2581 gttggcaatc tgctgcgccg taaggtcagt accgttagca atcttctctt tgtactggct
     2641 tgccatgtag aagaagcctt ccatctgcgg gttggtgctg aacagtttca gacccagcgc
     2701 ccaggtggtg cagataagca gccatacagc cggaacaaca gttacccaga tgtattgggt
     2761 gcgcttcatc ttaatcagca caacggtgcc cagtaccagc gctacggctg ccagcatctg
     2821 gttggagata ccgaacagcg gccacaggct cttaacgccg cccagcggat cgaccacgcc
     2881 ctgatacagc aggtagcccc acagacccac acagcccgca gtaccgatga taccagcaac
     2941 cagagagtcg gtttttttca ggaacgggat gaagttaccc agcaggtctt gcagcataaa
     3001 gcggccagaa cgggtacccg catccagcgc ggtcaggatg aacagggctt cgaacagaat
     3061 accgaagtga taccagaagc ccatgtcagc catcggcagc actttgtgga acacatgagc
     3121 gatacctacc gccagcgtcg gcgcaccacc tgcacggttc aggacagaag gctcaccaat
     3181 gtctttcgcg gtttgcagga tctgctctgg cgaaatcacg aagccccaag agctgacggt
     3241 cgctgccgcg tgtgcggtaa cgtctttcag ctgcgccatg atgatcggcg cgttctcgcc
     3301 acccatttca tgcaggttag gcatggtgat gccaaggccc gcgggcgggg tgttcatcgc
     3361 gaagtaaaga cccggttcga tgatggatgc agcaaccagc gccataatcg ccacgaagga
     3421 ctccatcagc attgcgccgt agccgatgaa acgcgcgtcg gtttcgttag ccagcagttt
     3481 cggcgtcgta ccggaagaga tcagcgcgtg gaagccagat accgcaccac aggcgatggt
     3541 gatgaacagg aacgggaaca gagcgccttt ccacagcggg ccagtaccgt caatgtactg
     3601 ggtcatcgca ggcattttca gttccgggtt cagcaccacg atacccagcg ccaggccgac
     3661 gataacgccg atcttcagga aggttgccag atagtcgcgt ggtgcgagga tcagccacac
     3721 cggcagcagc gcggaaacaa acgcatagcc aatcagcgcg aaggtaatgg tggtgtcttt
     3781 aaaggtcagt gccggacccc agtacggatc gtgagcaatc acgccaccga agtagataga
     3841 ggcaaccagc agcacgatac caataacaga gacttcaccc acacgccccg gacggataaa
     3901 gcgcatgtag atacccataa acagcgcaat tggtacggtt gagcaaacgg tgaagacacc
     3961 ccacggactt tctgccaggg ctttaaccac aatcagcgcc aggacggcga ggatgatgat
     4021 catgattaag aaacagccaa acagcgcgat agtccccggt actggcccca tctcttcttt
     4081 gatcatctca ccgagagatg cgccgttacg gcgagaggag ataaacagca ccataaagtc
     4141 ctgaaccgca ccggccagca ctacccccgc cagcagccac agcgtgccag gcaggtagcc
     4201 catctgcgcg gcgagaaccg gtcccaccag cggaccagca ccggcgatag cggcgaagtg
     4261 gtgaccaaac aacacgtaac ggttggtcgg aacgtagttc agaccgtcgt tgttaataac
     4321 cgcaggcgtc gcgcgcgtgg ggtcgagttt catcaccttc tgggcgatgt acagactgta
     4381 gtagcgatac gccaccagat acaccgacac agaggcgacc acgatccaca gggcgctgac
     4441 gtgctccccc cgacgtaatg caactaccgc gaggcagaat gcaccgatga ttccgagaat
     4501 cacccagggt atgtgcttga atagtttttt cgtatccata gtaaaacctg gcatgtattg
     4561 attaatagtt ggccgaagcc gttctaggtt tgcgttgcgt ttgaggaggt aaattgaccg
     4621 ctatcctgca atgatgctgc cagagttacg cgcgcgtaaa gtaaggtaaa taactgagtg
     4681 gttattttag ggacgtaagc ggtcagtttt gcggttgagc ggttttgagc aaggtttatg
     4741 aaggttattt atgtgatgat gatcacaacc gaagcgattt ttagccctcg gttgtgtaac
     4801 tcatttaaaa attatattca tactataaat attaaataac aatatagcaa tatggtgtgt
     4861 tatttatttc tttaagtaaa acgtgttttt tacgagattt agtaagataa aatttctcat
     4921 ctaaatagca atcgatgaga gagtccagcc tttccctgtc tatcataggg gtgataccca
     4981 aggtccagaa actgcgtaaa tcgagttcgg aacgttttat ttttgcgctt ttttctaagt
     5041 ctgcgatcat atttttaaca caactggtcg cgtctctgtt ttgttttatt tcaagcgcga
     5101 tatagctatc ttgtgcccag ccttttttac gtagtaagaa atctgtgcgt agtgtcgggc
     5161 gctcaggttt tttgcgcata tcacaggaaa gtgcttgttc acgccaccat tcatggtctg
     5221 ttgagcagag catattagca aactctacct ggaaccacac ttcccaaccg gtgatattgt
     5281 tttggtcaat aatattaagc ttttggcaaa tatcctcacg ttcaaaaaag agatgtaata
     5341 gcgttgagag atgatcgaag tcacggttta acatttcaga tgtcctttct taaaattcca
     5401 cactctataa aatatttcgt tca
//
LOCUS       DAZKIP010000208         5266 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZKIP010000208 DAZKIP010000000
VERSION     DAZKIP010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.210"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            3..404
                     /locus_tag="ACHOUK_004593"
     CDS             3..404
                     /locus_tag="ACHOUK_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001453516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899888.1"
                     /translation="MSFYEEKIRWRMRRQISNAEAIFYENQEEHIRRFLGRYTCRKPV
                     LVYWESEQRWTSVNGLEVMSMFGEHCHHIYLDDIKKKIQTELPENKTLDFKRQLQFII
                     LGDDKIRIWVPEGKLYFALMNVLQMFPMKNG"
     gene            529..906
                     /locus_tag="ACHOUK_004594"
     CDS             529..906
                     /locus_tag="ACHOUK_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899889.1"
                     /translation="MDERFINITPTSLSFKHADLSGLSYKNARLELALICPAIKSEVK
                     ISFDWIHSFRVTDEGDLLGMSGKQGINQTGIYRVANSTYLTWFTMQSCEIHKNKMIEH
                     YVIATSNDVIDVLSTVSPQIVYD"
     gene            1022..1822
                     /gene="ydcF"
                     /locus_tag="ACHOUK_004595"
     CDS             1022..1822
                     /gene="ydcF"
                     /locus_tag="ACHOUK_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFP9899890.1"
                     /translation="MNITPFPTLSTATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDVPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            2019..3458
                     /gene="aldA"
                     /locus_tag="ACHOUK_004596"
     CDS             2019..3458
                     /gene="aldA"
                     /locus_tag="ACHOUK_004596"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFP9899891.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(3500..4501)
                     /gene="gap"
                     /locus_tag="ACHOUK_004597"
     CDS             complement(3500..4501)
                     /gene="gap"
                     /locus_tag="ACHOUK_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFP9899892.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYKVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            4690..5220
                     /gene="cybB"
                     /locus_tag="ACHOUK_004598"
     CDS             4690..5220
                     /gene="cybB"
                     /locus_tag="ACHOUK_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFP9899893.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
BASE COUNT         1403 a         1156 c         1289 g         1418 t
ORIGIN      
        1 acttgtcttt ttatgaagaa aaaatccgct ggcgaatgcg tcggcaaatc tcgaatgcag
       61 aggcaatatt ttacgagaat caggaagaac acattcggcg ctttttaggc agatatacct
      121 gtcgcaaacc ggttcttgtt tattgggaaa gtgaacaaag atggacatct gtaaacggtc
      181 tggaagtgat gagtatgttt ggtgaacatt gtcatcacat ctaccttgat gatatcaaga
      241 aaaaaataca aactgaatta cctgaaaata aaactttaga tttcaaacgt caattgcaat
      301 ttataatatt aggggatgat aagattagaa tatgggtgcc tgaggggaaa ttgtatttcg
      361 cattaatgaa tgtattgcag atgtttccca tgaaaaatgg ttaataactg gacaagataa
      421 ttttaaaatt cagcaacggt tatcgattat acatggtact taaacagact cacaccattt
      481 ttgtagaagg ttaaattgag tacagtctca tatattaagg gtgttaacat ggatgaacgt
      541 tttataaata tcacgccgac ttcgttgtct tttaaacacg ccgatctgtc aggtttaagc
      601 tataagaatg ccaggcttga actggccctg atatgcccgg cgatcaagtc agaagttaag
      661 atatcatttg actggataca ctcatttcgt gttactgacg aaggtgattt gttaggtatg
      721 tcaggaaagc agggaattaa ccaaacgggg atctatagag tagccaattc cacttatctt
      781 acatggttta ctatgcaaag ttgtgaaatt cataaaaata aaatgatcga acattatgtt
      841 attgccacgt caaatgatgt tattgatgtt ttatcaacag tttctccgca aatagtgtat
      901 gactaaaacc agtgtctggt tcgcgatggc gagatagaaa aagcattatc ttcctttcag
      961 agtgctccgc ttctcactat tatctcacgc agtattctta agggaacgat aaggaggaac
     1021 catgaacatt accccgtttc cgacgctttc gacggcaact atagatgcca taaatgttat
     1081 cggacagtgg ctggcgcagg atgatttctc cggtgaggtg ccgtatcagg ctgattgcgt
     1141 catacttgca ggaaatgcgg ttatgccgac tatcgatgcg gcatgtaaga ttgctcgcga
     1201 tcagcaaatt cctttactga ttagtggtgg tatcggtcac tcgaccactt ttttgtatag
     1261 cgccatcgca cagcatccgc actacaacac tatccgcacc actggcagag cagaagcgac
     1321 catcctggcg gatatcgctc atcagttctg gcatattccg catgaaaaaa tctggattga
     1381 agaccagtca acaaactgcg gtgaaaacgc acgctttagc atcgcgctat tgaatcaggc
     1441 cgtagaacga gttcacacag ctatcgttgt acaggacccc accatgcagc ggcgcacgat
     1501 ggcgacgttc cgccgtatga ccggggacaa tcccgatgta ccacgctggt taagttatcc
     1561 cggattcgtt cctcagttag gaaataacgc agacagtgta atctttatta atcagttaca
     1621 aggattatgg ccggttgagc gttatctctc actactcact ggcgagctgc cgcgtttacg
     1681 cgatgatagc gatggctacg gtccccgcgg gcgagatttt atcgttcacg ttgattttcc
     1741 ggcagaagtc atccatgcat ggcaaacgct gaaacacgat gcggtgctca tcgaggcgat
     1801 ggaaagtcgc tcgttacgtt aaaaattgcc cgtttgtgaa ccacttgttt gcaaacgggc
     1861 ataactcctg ccttttattt ctgcctctta ttccttttac acttgttttt atgaagccct
     1921 tcacagaatt gtcctttcat gatgccgtct ctatgatgat tgatgttaat taacaatgta
     1981 tttaccaaaa acaaacatat aaatcacagg agtcgcccat gtcagtaccc gttcaacatc
     2041 ctatgtatat tgatggacag tttgttacct ggcgtggaga cgcatggatt gatgtggtaa
     2101 accctgctac agaggctgtc atttcccgca ttcccgatgg tcaggccgag gatgcccgta
     2161 aggcaatcga tgcagcagaa cgtgcacaac cagaatggga agcgttgcct gcaattgaac
     2221 gcgccagttg gttgcgcaaa atctccgccg ggatccgcga acgcgccagt gaaatcagtg
     2281 cgctgattgt tgaagaaggg ggcaagatcc agcagctggc tgaagtcgaa gtggctttta
     2341 ctgccgacta tatcgattac atggcggagt gggcacggcg ttacgagggc gagattattc
     2401 aaagcgatcg tccaggagaa aatattcttc tgtttaaacg tgcgcttggt gtgactaccg
     2461 gcattctgcc gtggaacttc ccgttcttcc tcattgcccg caaaatggct cccgctcttt
     2521 tgactggtaa taccattgtc attaaaccca gtgaatttac gccaaacaat gcgattgcat
     2581 tcgccaaaat cgtcgatgaa ataggccttc cgcgcggcgt gtttaacctt gttctggggc
     2641 gtggtgaaac cgttgggcaa gaactggcgg gtaacccaaa ggtcgcaatg gtcagtatga
     2701 caggcagcgt ctctgcaggt gagaagatca tggcgactgc ggcgaaaaac atcaccaaag
     2761 tgtgcctgga actggggggt aaagcaccag ctatcgtaat ggacgatgcc gatcttgaac
     2821 tggcagtcaa agccatcgtt gattcacgcg tcattaatag tgggcaagtg tgtaactgtg
     2881 cagaacgtgt ttatgtacag aaaggcattt atgatcagtt cgtcaatcgg ctgggtgaag
     2941 cgatgcaggc ggttcaattt ggtaaccccg ctgaacgcaa cgacattgcg atggggccgt
     3001 tgattaacgc cgcggcgctg gaaagggtcg agcaaaaagt ggcgcgcgca gtagaagaag
     3061 gggcgagagt ggcgttgggt ggcaaagcgg tagaggggaa aggatattat tatccgccga
     3121 cattgctgct ggatgttcgc caggaaatgt cgattatgca tgaggaaacc tttggcccgg
     3181 ttctgccggt agtcgcattt gacacgctgg aagatgctat ctcaatggct aatgacagtg
     3241 attacggcct gacctcatca atctataccc aaaatctgaa cgtcgcgatg aaagccatta
     3301 aagggctgaa gtttggtgaa acttacatca accgtgaaaa cttcgaagct atgcaaggct
     3361 tccacgccgg atggcgtaaa tccggtattg gcggcgcaga tggtaaacat ggcctgcatg
     3421 aatatctgca gacccaggtg gtttatttac agtcttaatg agtgaaagag gcggaggttt
     3481 tttcctccgc ctgtgcgcgt cagagtttag cgaatttttc gagggtgcga ataagctgcg
     3541 tgacgaagcc atattcgttg tcgtaccagg cgaccgtttt caccagttgt aaatcgccca
     3601 cggcggtaat ttccgtttgc gtggcatcaa acaccgaacc gaaatggctg ccaatgatat
     3661 cggaagagac tatttcttca tcggtataac caaatgactc gttattggtg gttgcttgtt
     3721 taagtgcgtt attcacctct tcggcagtca ctttttttcc gagaatcgat accagttcag
     3781 tgaccgaacc tgttttcacc ggcacgcgtt gcgcatgacc tttcagtttg ccgctcagtt
     3841 ccgggatcac cagaccaatg gcttttgccg cgcccgtagt gtggggaatg atattttctg
     3901 ccgcggcgcg tgaagcacgt aaatctttac cacgcgggcc atccaccagt gactgggtgc
     3961 cagtataggc atgaatggtc gtcatcgtgc cgacttctat cccgaaactg tcatgcaagg
     4021 ctttggccat cggcgcaaga cagttagtag tgcatgatgc cacggaaaca atggtgtcgt
     4081 tgccatccag agtgtcgtca ttgactttat aaacgatagt tttcatttca cctgcagggg
     4141 cggaaatcaa caccttcttc gcgccagcat caagatgcgc ctgcgatttc tcggcggagg
     4201 tataaaagcc agtacattcg acaatgattt ctgcaccttt cgctttccac ggaatatttt
     4261 tagcctcttt ttcggcgtaa accgcgatac ttttcccatc aacgataagt gaatcttccg
     4321 taaaatcaac gctccagggg aacggaccat agtttgaatc atgtttcagc agataggcga
     4381 gaatttttgg ggaagtaaga tcattaatag cgacaacgtc tatgttgctt ttgacttcaa
     4441 gtaatcgacg caacaccagt cgaccgatac gaccaaaacc gttaatacca actttactca
     4501 tggttttctc ctgtcaggaa cgttcggatg aaaattgatc ctttccaggc ttagaccagg
     4561 atggcgggat gggcaatctc cattcttaca gtgaaacgta acgtactgaa aaagggtgaa
     4621 caatatttaa tgaaattttg agaaaagccc gttatgttaa cggaaaatta tgttaaagca
     4681 ggaaatgtta tggaaaataa atactcaagg ttacaaatca gcattcactg gctggtcttt
     4741 ttgctggtta tcgcagcgta ttgcgcaatg gagtttcggg gtttcttccc acgtagcgat
     4801 cggccactca tcaacatgat tcatgtttcc tgcggcatat caattctcgt gctgatggtc
     4861 gttcgtcttt tgttaaggct gaaataccca accccgccga ttatacctaa gccaaaaccg
     4921 atgatgacgg gactggcgca tttgggacat ttggtgattt atctcctgtt tattgcgctg
     4981 ccagtgattg gtttggtgat gatgtataac cgaggcaacc cgtggtttgc gtttggtttg
     5041 acgatgcctt acgcttcaga ggccaatttc gaacgggtag atagcttaaa gtcgtggcat
     5101 gaaacgctgg cgaatctggg atattttgtc atcggtttgc acgctgcggc tgcactggca
     5161 caccactatt tctggaagga caacacactt ctacgcatga tgccgcgtaa acgttcctga
     5221 aggatattta aagaaaacgc ctgtactaaa accgacccgt ggtaca
//
LOCUS       DAZKIP010000209         5246 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZKIP010000209 DAZKIP010000000
VERSION     DAZKIP010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.211"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..1477)
                     /locus_tag="ACHOUK_004599"
     CDS             complement(<1..1477)
                     /locus_tag="ACHOUK_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscV/YscV/HrcV family type III secretion system
                     export apparatus protein"
                     /protein_id="HFP9899894.1"
                     /translation="MFNKVLVGLRSHPELIILGLMVMIIAMLIIPLPTYLIDFLIGLN
                     LTLAILVFLGSFYVDRILSFSSFPSILLITTLFRLALAISTSRLILLEADAGEIITSF
                     GEFVIGDSLVVGFVIFSIVTIVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLR
                     AGIIDADLAKERRSVLERESQLYGSFDGAMKFIKGDAIANIIIIFVNIIGGLSVGVGQ
                     NGMDFSTALTVYTILTVGDGLVSQIPALLIAISAGFIVTRVNGDSDNMGQNIMSQLLS
                     NSFVIVVTCVLALSIGLLPGFPLLVFLCLAVILGIYFYFKFKKKGTEETVVEGDITVG
                     LEPYNQDDDISLGIINKLDQVITETVPLVLIMNSVQAKKYTEINLADRIRSQFFIEYG
                     IRIPGIVIREGEGLNDEDVILMLNEVRASQFKIYHDLVLLVEYSDEVVSTLIKKPVIV
                     NSNGEQYYWVTKSDAQKLTKIGCYTRTAMDEMYNHLSVCLSG"
     gene            complement(1470..2615)
                     /locus_tag="ACHOUK_004600"
     CDS             complement(1470..2615)
                     /locus_tag="ACHOUK_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopN/LcrE/InvE/MxiC type III secretion system
                     gatekeeper"
                     /protein_id="HFP9899895.1"
                     /translation="MAIHVEHVGVLERAREVSRLEDIITEDNEDIEAEMPKMRDDPAG
                     KEARFLQATDEMSAALTQFMKKKIYEEQLANFLDGEEYVLEDQPIEKTDKVMEALKAA
                     TTHDYEVYSFAKKLFPDESDLVVVLRAILRKKQISENVRLNAEALLRKVNQETTKKFI
                     NSGINSALKAKLFGQALSLNPKLLRASYRQFLMAEDDAVDTYVEWIGSYGYQNRMLVT
                     KFIKETLFSDINALDASCSSLEFGMFLNKLSQLLSLQSAEALFLKTLMNNPIIKKFIS
                     AEDYWIFFLISLIKFPETAEELLNNALVTLPADANYKDKTLLLKAIYSGCTNLPFSLF
                     INNEQLLEIRECCKQAIKVTFAAELFDTQNCNKKQNKKPWKKVMFNV"
     gene            complement(2620..4323)
                     /gene="sctC"
                     /locus_tag="ACHOUK_004601"
     CDS             complement(2620..4323)
                     /gene="sctC"
                     /locus_tag="ACHOUK_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system outer membrane ring
                     subunit SctC"
                     /protein_id="HFP9899896.1"
                     /translation="MKIKLRITIILISVLCIFNGLLTPGAYAAAANGYVANKENLRSF
                     FETVSSYAGKPTIVSKLAMKKQISGNFDLTEPYALIERLSAQMGLIWYDDGKAIYIYD
                     SSEMRNALINLRKVSTNEFNNFLKKSGLYNSRYEIKGDGNGTFYVSGPPVYVDLVVNA
                     AKLMEQNSDGIEIGRNKVGIIHLVNTFVNDRTYELRGEKIVIPGMAKVLSTLLNNNIK
                     QSTGVNVLSEISSRQQLKNVSRMPPFPGAEEDDDLQVEKIISTAGAPETDDIQIIAYP
                     DTNSLLVKGTVSQVDFIEKLVATLDIPKRHIELSLWIIDIDKTDLEQLGADWSGTIKI
                     GSSLSASFNNSGSISTLDGTQFIATIQALAQKRRAAVVARPVVLTQENIPAIFDNNRT
                     FYTKLVGERTAELDEVTYGTMISVLPRFAARNQIELLLNIEDGNEINSDKTNVDDLPQ
                     VGRTLISTIARVPQGKSLLIGGYTRDTNTYESRKIPILGSIPFIGKLFGYEGTNANNI
                     VRVFLIEPREIDERMMNNANEAAVDARAITQQMAKNKEINDELLQKWIKTYLNREVVG
                     G"
     gene            complement(4320..5069)
                     /locus_tag="ACHOUK_004602"
     CDS             complement(4320..5069)
                     /locus_tag="ACHOUK_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9899897.1"
                     /translation="MIEEGLLLPMNVLLQGQKLTLLDPEMWFLRGNENKDVTLVITIG
                     NNHQKLMVVEDILLLIDRSQIEVTAGKVIYHPLRIDILSKLLAFIDESTGSVEREHHE
                     LFADALPFASEVVLFSKVASEAWFLATYLSSDNINEILFQHLRKTECYKLVRYLLSQS
                     LIQTSLYDLGELYGVSYSHFRRLCSYALGGKVKTELCGWRVARAVLEIIEGNSDMTTI
                     AHKYGYSSSSHFSAEVKSRLGKTPRELCKKL"
BASE COUNT         1596 a         1123 c          849 g         1678 t
ORIGIN      
        1 agccagacag acagacagat aaatgattat acatttcatc catggccgtg cgagtataac
       61 agccaatttt tgttaatttt tgagcatcac ttttcgtaac ccaataatat tgttcaccat
      121 tactatttac aatgacaggt ttcttgatca aagtcgaaac cacttcatct gaatattcaa
      181 ccaacaagac aagatcatga taaattttaa attgtgaagc tctgacttca tttagcatta
      241 atatgacatc ttcatcatta agaccttccc cctcacgaat gacaataccg ggaatgcgga
      301 taccatactc aataaaaaac tgactacgaa tcctatcagc aagattgatt tcagtgtatt
      361 ttttagcctg tacactattc ataatcaaga ccaagggtac cgtttcggta ataacctgat
      421 caagtttatt aatgatacct aatgagatat catcatcttg attatagggt tccaacccaa
      481 cggtaatatc tccctcaaca accgtttctt cagtcccttt ctttttgaat ttaaagtaaa
      541 agtaaattcc taagattact gccaaacata aaaacactag aaggggaaaa cctggaagca
      601 acccaatact tagcgcgagg acacatgtca caacaatgac aaaagaatta cttaataact
      661 gggacattat attttgcccc atattatcac tatcgccatt tacgcgcgta acgataaatc
      721 ctgcgctaat agcaattaat aaagctggaa tttgagaaac aagaccatca ccgacagtaa
      781 gaattgtgta gacagtcaac gctgttgaga aatccattcc attttggcct actcctactg
      841 atagcccacc gataatatta acaaagataa taataatgtt tgcaatagca tcacctttga
      901 taaatttcat cgcaccatca aaggaaccat acaactggct ctctcgttct aatacactac
      961 gacgttcttt tgctaaatct gcatcaatga tcccggcgcg tagatcggcg tcaatgctca
     1021 tttgtttacc tggcataccg tcaagcgaaa agcgggcagc aacttcagcc acacgttccg
     1081 aacctttagt aatcacgata aactgaacaa tagtaacaat ggagaaaata acaaaaccaa
     1141 ctaccaggct atctccaata acgaactcac caaaactcgt tataatttct ccagcatccg
     1201 cctcaagaag tataagtcga ctggtactga tcgccagcgc caaacgaaat aacgttgtga
     1261 taaggaggat tgatggaaat gaagaaaagc ttaagatcct atcaacatag aatgatccta
     1321 aaaatacaag tattgccaat gttaagttaa gcccaatcaa aaagtcgatt aaataggtgg
     1381 gcaatggaat tatgagcatg gcgataatca tcaccattaa tccaagaatt attaattctg
     1441 gatgactcct taaccctacc aaaactttgt taaacattga acatcacctt cttccatggt
     1501 tttttatttt gttttttgtt acagttttgt gtatcaaaga gctctgctgc aaatgttact
     1561 tttattgcct gtttacaaca ctccctaatc tctaataatt gttcattatt aataaaaagt
     1621 gaaaatggaa gatttgtaca tccactgtaa atcgccttca ggagtaacgt tttgtcttta
     1681 taattagcat ctgcgggaag ggtcaccagt gcattgttca ggagttcctc tgcagtctca
     1741 ggaaatttta ttaacgatat cagaaaaaat atccagtaat cttctgcgct gataaatttt
     1801 ttaattattg ggttattcat taaagttttc agaaataaag cctctgccga ctgaagagac
     1861 aatagttgtg acaacttatt gagaaacata ccaaattcca atgatgaaca actcgcatca
     1921 agtgcattta tatctgagaa aagcgtctct ttgataaatt ttgtcaccaa cattctattt
     1981 tgatagccat aactaccaat ccattcgaca taagtatcta cagcatcgtc ttctgccatt
     2041 aaaaattgcc gataactcgc ccttagtaat tttggattta atgacagcgc ctggccaaat
     2101 aactttgctt ttaatgccga gttaattccg ctattaatga attttttagt agtctcttga
     2161 ttaacttttc taagtaatgc ttcagcgttt agccggacat tttctgatat ttgtttttta
     2221 cgtaaaattg cacgtaacac aactaccaga tcgctttcat ctgggaataa ttttttagca
     2281 aagctataca cttcatagtc atgggtagtt gcagccttaa gcgcctccat aactttgtcg
     2341 gttttttcta ttggctggtc ttccaggaca tattcttctc cgtccagaaa attggccagt
     2401 tgttcttcgt aaattttttt cttcataaac tgcgttaacg ctgctgacat ctcgtcagta
     2461 gcttgtaaga accgagcttc tttacctgcg ggatcatccc tcattttagg catttcagct
     2521 tcaatgtctt cattatcttc tgtgataatg tcctctaagc gagaaacttc cctggctctt
     2581 tctaatacac cgacatgctc tacatgaatc gccatttcac tatcctccga cgacttcacg
     2641 attcaggtaa gtttttatcc atttctgcag taattcatcg ttgatttctt tattttttgc
     2701 catttgctgt gtaatcgctc tggcatcaac cgcagcctca ttcgcattat tcatcatgcg
     2761 ttcatcgatc tctcttggct caataagaaa gacacgaacg atgttattcg catttgttcc
     2821 ttcataacca aataatttac caataaatgg aatgctgcct aatattggga tcttgcgact
     2881 ttcataggta ttcgtatcgc gtgtatatcc cccaatcaaa agacttttcc cttggggcac
     2941 gcgtgcaata gtacttataa gtgttctacc aacctgaggc agatcatcaa cattggtctt
     3001 gtcggaattg atttcatttc catcttcaat attcaagagt aattcaattt gatttcgcgc
     3061 agcaaatcgt ggtagcacgc taatcatcgt accataggtt acttcatcca attccgcagt
     3121 acgctcgccg actaatttag tatagaaagt gcggttatta tcaaatatag caggaatatt
     3181 ctcttgtgtt aaaacgacgg gacgagcaac aacggcggct cttctttttt gtgcaagtgc
     3241 ctgtattgtt gcaataaact gtgtcccatc aagggtacta attgaacctg aattattaaa
     3301 tgatgcactt aagctggatc caatttttat cgtgcccgac cagtcagcac ctagctgttc
     3361 tagatcagtc ttatcaatat ctattatcca taaagataat tcaatgtgtc gctttggaat
     3421 atcaagcgta gctactaact tctcgataaa atcaacctga gatactgttc ctttaacaag
     3481 taaactgttg gtatcaggat atgcaataat ttgtatatcg tcagtctctg gcgctcctgc
     3541 tgtcgatatt attttctcta cttgtaggtc atcatcttct tcagccccag gaaaaggagg
     3601 catacgtgat acgtttttta gttgttgacg acttgaaatt tcagaaagta cgttcacccc
     3661 tgtgctttgc ttaatgttat tatttaacaa cgtcgatagt acttttgcca tgccaggaat
     3721 aactatcttt tcgccacgta actcataagt ccggtcatta acgaatgtgt tgaccagatg
     3781 aattattcct actttatttc gaccgatctc aatgccatcg ctgttttgct ccattaattt
     3841 cgcagcatta acgaccaggt caacataaac cggtggcccc gatacataga aagtaccatt
     3901 gccgtcccct ttaatttcat aacgagagtt atacagaccc gattttttta agaaattatt
     3961 aaactcattc gtcgatacct ttctcagatt aatcaatgca ttacgcatct ctgatgagtc
     4021 atagatataa atagctttgc catcatcata ccaaataagc cccatctgtg ccgacaggcg
     4081 ttcaatcaag gcatagggtt ctgttaaatc aaaatttccg ctgatctgtt tcttcatggc
     4141 aagcttacta actattgtag gcttacccgc atatgatgaa accgtttcaa aaaaactacg
     4201 tagattttct ttatttgcta cgtatccatt cgctgctgcg gcatatgcac caggagtcaa
     4261 taatccatta aaaatgcata agactgaaat taatataata gtaatgcgta attttatttt
     4321 cataactttt tacatagttc tctcggtgtt tttcctaacc ggctttttac ttcggctgaa
     4381 aagtgtgatg aggacgagta accatattta tgcgcaatag ttgtcatatc actgtttcct
     4441 tcaatgattt ccaacacggc tctggcaact ctccagccac ataattccgt tttaacttta
     4501 ccacccaatg cataactaca taaacggcga aaatgtgaat acgatacgcc gtaaagctca
     4561 ccaagatcat agagtgaagt ctgtatgagc gattgcgata ataaataacg aaccagttta
     4621 tagcattctg tttttcttag atgctgaaac aggatttcat taatattatc gctgctcaga
     4681 taagtggcta aaaaccaggc ttcacttgca actttactga atagcacaac ttctgatgca
     4741 aagggcaagg catcagcaaa caattcatgg tgttcccttt ccacactccc cgttgattca
     4801 tctataaaag ctaataattt actcaggata tcaatgcgaa gtggatgata aattactttc
     4861 cctgcagtta cctcaatttg gcttcggtca atgagcagta atatatcttc cactaccatt
     4921 aatttttgat ggttattacc aatcgtaata acaagggtta catccttgtt ttcatttccc
     4981 cgtaagaacc acatttcagg gtccagtagg gttaacttct gtccttgaag caaaacattc
     5041 attggaagta acagcccttc ttcaatcatg tttcatctcc ttattccata ttctcattct
     5101 ccataaactt gctataaaaa catgattaaa aagtgatgat tactatttat aaaataatga
     5161 ttttgttatg agcgaatagc tatcagccac ttacactagt acgtcatcct gtccatatac
     5221 agatttgtaa ctgttgatat gtttta
//
LOCUS       DAZKIP010000210         5221 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZKIP010000210 DAZKIP010000000
VERSION     DAZKIP010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5221
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.212"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            138..4040
                     /gene="espP"
                     /locus_tag="ACHOUK_004603"
     CDS             138..4040
                     /gene="espP"
                     /locus_tag="ACHOUK_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter EspP"
                     /protein_id="HFP9899898.1"
                     /translation="MNKIYSLKYSHITGGLIAVSELSGRVSSRATGKKKHKRILALCF
                     LGLLQSSYSFASQMDISNFYIRDYMDFAQNKGIFQAGATNIEIVKKDGSTLKLPEVPF
                     PDFSPVANKGSTTSIGGAYSITATHNTKNHHSVATQNWGNSTYKQTDWNTSHPDFAVS
                     RLDKFVVETRGATEGADISLSKQQALERYGVNYKGEKKLIAFRAGSGVVSVKKNGRIT
                     PFNEVSYKPEMLNGSFVHIDDWSGWLILTNNQFDEFNNIASQGDSGSALFVYDNQKKK
                     WVVAGTVWGIYNYANGKNHAAYSKWNQTTIDNLKNKYSYNVDMSGAQVATIENGKLTG
                     TGSDTTDIKNKDLIFTGGGDILLKSSFDNGAGGLVFNDKKTYRVNGDDFTFKGAGVDT
                     RNGSTVEWNIRYDNKDNLHKIGDGTLDVRKTQNTNLKTGEGLVILGAEKTFNNIYITS
                     GDGTVRLNAENALSGGEYNGIFFAKNGGTLDLNGYNQSFNKIAATDSGAVITNTSTKK
                     SILSLNNTADYIYHGNINGNLDVLQHHETKKENRRLILDGGVDTTNDISLRNTQLSMQ
                     GHATEHAIYRDGAFSCSLPAPMRFLCGSDYVAGMQNTEADAVKQNGNAYKTNNAVSDL
                     SQPDWETGTFRFGTLHLENSDFSVGRNANVIGDIQASKSNITIGDTTAYIDLHAGKNI
                     TGDGFGFRQNIVRGNSQGETLFTGGITAEDSTIVIKDKAKALFSNYVYLLNTKATIEN
                     GADVTTQSGMFSTSDISISGNLSMTGNPDKDNKFEPSIYLNDASYLLTDDSARLVAKN
                     KASVVGDIHSTKSASIMFGHDESDLSQLSDRTSKGLALGLLGGFDVSYRGSVNAPSAS
                     ATMNNTWWQLTGDSALKTLKSTNSMVYFTDSANNKKFHTLTVDELATSNSAYAMRTNL
                     SESDKLEVKKHLSGENNILLVDFLQKPTPEKQLNIELVSAPKDTNENVFKASKQTIGF
                     SDVTPVITTRETDDKITWSLTGYNTVANKEATRNAAALFSVDYKAFLNEVNNLNKRMG
                     DLRDINGEAGAWARIMSGTGSASGGFSDNYTHVQVGVDKKHELDGLDLFTGFTVTHTD
                     SSASADVFSGKTKSVGAGLYASAMFDSGAYIDLIGKYVHHDNEYTATFAGLGTRDYST
                     HSWYAGAEAGYRYHVTEDAWIEPQAELVYGSVSGKQFAWKDQGMHLSMKDKDYNPLIG
                     RTGVDVGKSFSGKDWKVTARAGLGYQFDLLANGETVLRDASGEKRIKGEKDSRMLMSV
                     GLNAEIRDNVRFGLEFEKSAFGKYNVDNAVNANFRYSF"
     gene            complement(4296..4777)
                     /locus_tag="ACHOUK_004604"
                     /pseudo
     CDS             complement(4296..4777)
                     /locus_tag="ACHOUK_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         1573 a         1142 c         1177 g         1329 t
ORIGIN      
        1 tgtttgttat ccgtatgata acaaacacat aaaggagtaa acatttatct ggacgatgat
       61 tacaatgttc acgactgtca gagacacacc tctgatacat tagaaaaacc aatgtttccc
      121 ttaaaaatgg agcttatatg aataaaatat actctcttaa atacagccat attacaggag
      181 ggttaatcgc tgtttctgaa ttatccggca gagtatcatc aagagcaact ggtaagaaaa
      241 aacacaaacg catacttgca ttatgttttt taggcttatt acaatcctca tattcttttg
      301 cgtcacagat ggatatttca aatttctaca tccgtgacta tatggatttt gcacagaaca
      361 agggcatatt tcaggctggc gcaacaaata ttgaaatagt gaagaaagat ggctccaccc
      421 tgaaactacc ggaagtacca tttcctgact tctcaccggt tgcaaacaaa gggtcaacca
      481 catctattgg tggtgcatac agtatcacag ccacacacaa tacgaaaaac caccactcag
      541 ttgcgacgca aaactggggg aacagcacgt acaaacaaac tgactggaat acttcacatc
      601 ctgattttgc agtatcccga cttgacaagt ttgttgttga gacccgaggt gcgactgaag
      661 gcgcagatat ttcgttatca aaacagcagg cacttgaacg ttacggggtt aattataaag
      721 gagaaaagaa acttatcgca ttcagagccg gctctggtgt ggtatccgtt aaaaaaaatg
      781 gacgcataac tccatttaat gaggtttctt ataagccaga aatgttaaat ggctctttcg
      841 ttcacattga tgactggagt ggatggctga tattaaccaa caaccagttt gatgagttta
      901 ataacattgc ctctcagggt gacagcggtt cagcactgtt cgtctatgat aaccaaaaga
      961 aaaagtgggt tgtcgctgga actgtctggg ggatttataa ttacgccaat ggcaaaaacc
     1021 acgcagcata cagtaaatgg aaccagacaa ccattgacaa cctgaagaac aagtattctt
     1081 acaacgtgga tatgtcaggg gctcaggttg caaccattga aaatggaaaa ctgacaggca
     1141 ctggctcaga caccaccgat ataaaaaata aggacttaat atttactggc ggtggagata
     1201 tcctcctgaa atcctctttt gataatggtg ctggcggtct tgtctttaat gataaaaaga
     1261 cctatcgagt aaacggggat gatttcacct ttaaaggtgc cggtgttgat acaagaaacg
     1321 gcagcaccgt tgagtggaat atccggtatg ataataaaga caaccttcac aaaattggtg
     1381 atggcacatt agatgtccga aaaacccaga acaccaacct gaaaacaggt gagggtcttg
     1441 tcattcttgg agctgaaaaa acattcaata atatctacat aaccagtggt gatggaactg
     1501 tccgactgaa tgcagaaaat gcactgtctg gcggtgaata caacggtatt ttctttgcga
     1561 aaaatggcgg aactcttgac ctgaacggat ataatcagtc tttcaataaa attgctgcaa
     1621 ctgattcagg tgctgtaata accaatacgt caaccaaaaa atccatttta tccctgaata
     1681 atactgctga ctatatctat cacggcaaca taaacgggaa tctggacgta cttcagcatc
     1741 atgagacgaa aaaagagaac cgtcgtctta ttcttgatgg gggcgtggac acaacaaatg
     1801 atataagcct gcgtaataca caactgtcca tgcagggaca tgccactgaa catgccattt
     1861 atcgggatgg agctttctct tgttcactac cagctcctat gcgctttttg tgtggcagtg
     1921 attatgttgc aggaatgcaa aatacagaag ctgatgctgt aaaacaaaac ggaaatgcct
     1981 ataaaaccaa caatgctgtc tctgatttat cgcagccaga ctgggaaacc ggaacattca
     2041 gatttggaac gctacatctt gaaaattccg atttttctgt tggtcgtaat gcaaatgtaa
     2101 tcggggacat tcaggccagt aaatcaaaca ttactattgg tgacactaca gcatatattg
     2161 atttgcatgc tggtaaaaat attaccggtg atggttttgg cttccgccag aatattgtgc
     2221 gtggaaactc acaaggagaa acgctgttta caggagggat cacagcagaa gacagcacta
     2281 tcgttattaa agataaagca aaagcattat tttcaaatta tgtatacctg ctgaacacaa
     2341 aagcaaccat agagaacggt gctgatgtga caactcaaag tggtatgttc tccacgagcg
     2401 atatcagcat ctctggtaat ctgtccatga caggcaatcc cgacaaagac aataaattcg
     2461 agccctcaat atatctgaat gatgcttctt atctactgac tgacgactcc gccagactcg
     2521 ttgccaaaaa taaagcatct gtggtgggag atatacactc cactaaaagt gcatccatca
     2581 tgtttggtca tgatgaaagc gacctctcgc agttgtctga cagaacctca aaagggcttg
     2641 cacttggtct tttaggtggc tttgatgtct catatcgcgg ttcagtcaat gccccgtcag
     2701 catctgccac tatgaacaac acctggtggc aactaaccgg agattctgcg ctgaaaacac
     2761 tgaaaagtac aaacagcatg gtctatttca ctgacagcgc aaacaataag aaattccata
     2821 cgctgacggt cgatgagctg gcaaccagca acagcgccta tgcgatgcgt acaaaccttt
     2881 ctgaatcaga caaactggag gtcaaaaaac acttgtctgg tgagaacaat attttactcg
     2941 ttgatttcct tcagaaacca acgcctgaaa aacaactgaa tattgaactg gtaagcgcgc
     3001 caaaagacac caatgaaaat gtctttaaag ccagtaaaca aaccattggt ttcagtgatg
     3061 taacgccggt cattacaacc agggaaaccg atgacaaaat aacatggtca ctgacaggct
     3121 ataacacggt agcaaacaag gaagcaaccc ggaatgccgc cgccctgttc tctgttgact
     3181 ataaagcgtt tctgaacgag gtcaacaacc tgaacaaacg tatgggtgac ctgcgtgata
     3241 tcaacggcga agccggtgca tgggcacgca tcatgagcgg taccggctct gccagtggtg
     3301 gtttcagtga caactacacg cacgttcagg tcggggtcga caaaaaacac gagctggacg
     3361 gactggattt gtttaccggt ttcactgtca cacacactga cagcagtgcc tccgccgatg
     3421 ttttcagtgg taaaacgaag tctgtggggg ctggcctgta tgcttccgcc atgtttgatt
     3481 ccggtgccta tatcgacctg attggcaagt atgttcacca tgataatgag tacactgcaa
     3541 cctttgccgg actcggaacc cgtgattaca gcacgcattc atggtatgcc ggtgcagaag
     3601 cgggctaccg ctatcatgtc actgaggatg cctggattga gccacaggct gagctggttt
     3661 acggttctgt atccggtaaa cagtttgcat ggaaggacca gggaatgcat ctgtccatga
     3721 aggacaagga ctacaatccg ctgattggcc gaacgggtgt ggatgtgggt aaatccttct
     3781 ctggtaagga ctggaaagtg acagcccgtg ccggtctggg ctaccagttc gacctgctgg
     3841 ctaacggcga aaccgtattg cgggatgcat ctggtgaaaa acgcatcaaa ggtgaaaagg
     3901 acagccgtat gctgatgtcc gttggcctga atgcagaaat cagggataac gtccgctttg
     3961 gactggagtt tgagaaatcc gcctttggta agtacaacgt tgataatgct gtcaacgcta
     4021 atttccgtta ctcgttctga tactccgtat tcaaaagggc ctgcgggccc tttacgctca
     4081 gtgcctgccg ccctgatatg tctgagagac atttccggga tgtcagtcga ctggtttact
     4141 taactgcgat gactaaagct ggcagtaatg ccggcttttt tacggccaca tacgtcttcc
     4201 tctcatccgg aactgtgata caaatcctgc atacaggatt gaccgccggt tcctgcaaat
     4261 ggcacatttt cagtaagata gcgaaggtta catttgtcac tggttaacat accgatggta
     4321 aaaggggcaa gcagcgccag caattcatca gcccgggggc caaaagtgta ggccagaacg
     4381 ccaccagttt tcgtgttgta cgcgtaccag agcctgtgct gtcacgcttt actgccgata
     4441 aaactccatt gctcatcaag ttcgcagatg agtgccatat cagcatgagc gaccggggat
     4501 gacgttattc ttcgtggcga gcgtttttta aagtccgaat gacagtatta atgccgattt
     4561 tcagcgtacg ggcagtatcg cgaactccgg caccgttgaa aggcatttca gtgatttggt
     4621 cttgaactcc cggtttacgg gcctcgtaac ggtaagtgag ctgaaacact cgatgacagt
     4681 cacggcagcg aaatcgatca tgtccttttg ggttcttccc gtgacggtaa acctgaacgg
     4741 actggcaacg ggggcaatga aggttaactg tgaccattaa agaaccttaa aagtgactat
     4801 tataccccta attcaactaa ttggaggcat taccatgcat tagtgcaagc gccttgtgga
     4861 ttcttttgct aatggcggaa acatattgta caatattgca tttatccctc aataaataaa
     4921 tgagttatca gattgcaaaa atctgttatc aaactaattc aaataaaaag agattgatac
     4981 gaccaacgta cacaaaaatt ttcgatattc gaactaaata acagctccta ataccgctac
     5041 actttagcta tccagtctgc gcctcagaaa tggacgatgc cgttgaagga ctggtgtatg
     5101 gcaatgagcc gctttattat cgaattcggt gaccgcttgg acggtcactt cggagaaaag
     5161 gcatttacac agcatcctaa acaggttcag cataggtgat ataccgcctt ttcattttct
     5221 t
//
LOCUS       DAZKIP010000211         5120 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZKIP010000211 DAZKIP010000000
VERSION     DAZKIP010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5120
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.213"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..114)
                     /locus_tag="ACHOUK_004605"
     CDS             complement(<1..114)
                     /locus_tag="ACHOUK_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein TerF"
                     /protein_id="HFP9899899.1"
                     /translation="MNLQSGQNIPLQQSTIRLNLQYPAKSGFKGEPDTCLFM"
     gene            complement(537..1112)
                     /locus_tag="ACHOUK_004606"
     CDS             complement(537..1112)
                     /locus_tag="ACHOUK_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFP9899900.1"
                     /translation="MAVSLVKGGNVSLTKEAPTMNVAMVGLGWDARVTDGQGFDLDAS
                     VFAVGEDGKVLSDAHFIFFNNKTSPDGAVEHQGDNRTGEGDGDDEQVKIDLTKVSADI
                     KKLVFAVTIYDAEARKQNFGMVSNSFMRVYNNDNGTEIARFDLSEDASTETAMVFGEL
                     YRHGTEWKFKAVGQGFAGGLSALASQHGVNV"
     gene            complement(1181..1759)
                     /gene="terD"
                     /locus_tag="ACHOUK_004607"
     CDS             complement(1181..1759)
                     /gene="terD"
                     /locus_tag="ACHOUK_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerD"
                     /protein_id="HFP9899901.1"
                     /translation="MSVSLSKGGNVSLSKAAPSMKNVLVGLGWDARSTDGQDFDLDAS
                     AFLLASNGKVRGDSDFIFYNNLTSSDGSVTHTGDNRTGEGDGDDESLKIKLDAVPSEV
                     DKIIFVVTIHDAQARRQSFGQVSGAFIRLVNDDNQTEVARYDLTEDASTETAMLFGEL
                     YRHNGEWKFRAVGQGYAGGLASVCAQYGINAS"
     gene            complement(1808..2848)
                     /gene="terC"
                     /locus_tag="ACHOUK_004608"
     CDS             complement(1808..2848)
                     /gene="terC"
                     /locus_tag="ACHOUK_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerC"
                     /protein_id="HFP9899902.1"
                     /translation="MVSTHIGFPTETVIVFIALSVGAIFIDLFMHRDDKPISLKSAAL
                     WSVFWVVVAMAFAGFLYIHHGAEVASLFVTGYALEKVLSVDNLFVMMAIFSWFAVPDR
                     YRHRVLYWGIIGAIVFRGIFVAIGTSLLSLGPYVEVVFAIIVAWTAVMMLKSGDDDDE
                     IEDYSQHLAYRMVKRFFPIWPKLRGHAFLLNQKEVDAELAKPENSDVTIGRGKKAALY
                     ATPLFLCVAVVELSDVMFAFDSVPAIIAVSREPLIVYSAMMFAILGLRTLYFVLEALK
                     QYLVHLEKAVIVLLFFIAAKLGLNATDHIWHHGYSIAATTSLYVVLGVLALGILASVM
                     FPGKPESEEKGS"
     gene            complement(2871..3326)
                     /locus_tag="ACHOUK_004609"
     CDS             complement(2871..3326)
                     /locus_tag="ACHOUK_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFP9899903.1"
                     /translation="MSFFDKVKGAINSGRDELTRQVGRFKNKKFMQGTVAVCARIAVS
                     SDGVSSEEKQKMMGFLRSSEELKVFDTNEVIEFFNKLVSSFDFDVEIGKGETMKYILA
                     LKDQPEAAQLALRVGIAVAKSDGNFDQDEKLASREIAIALGFDPAEFGL"
     gene            complement(3349..4506)
                     /locus_tag="ACHOUK_004610"
     CDS             complement(3349..4506)
                     /locus_tag="ACHOUK_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFP9899904.1"
                     /translation="MNLTPGGNAPVPAQELRVRITSGGQVDASAFRLYADGKVQGDAD
                     MVFYGQPRNDDGTVSLVSEGQYSTFTVALNRLKPDVQKIAFTVTCDGGQTVSGLRNLS
                     IDVEQGATGLVSGSVELSGRQEAALILGEFYRRNNDWKFRFVAQGFNGGLKPLAEHFG
                     VNIADEPAPAAPTPPVVTPPPVETKISLSKVSLTKEKPAISLTKRDNFGEIRINLNWH
                     RGSSKSGFAGMFGSKGIDLDLGAFVELQDGYKSVIQALGNAFGDYRDEPYVQLKGDDR
                     TGDVSDGEWLHINGREWKHIREVLIYAFIYEGVPSWDKTDGVVTIHVPDQPPIETRLT
                     EGENRRTLCAIARLVNENGAIKVERINQYFKGQDEMDRAFGWGFRWSAGSK"
     gene            complement(4506..5087)
                     /locus_tag="ACHOUK_004611"
     CDS             complement(4506..5087)
                     /locus_tag="ACHOUK_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFP9899905.1"
                     /translation="MVSLVKNQTVSLSKESSALSQLHFGLGWDPVKKKGLLGGLFGGN
                     DSIDLDAGCVLMDSTGKTIDTIWFRKLKSTCGAVVHSGDNLTGEGDGDDEVIKVNLSR
                     LPANVEYLAFTVNSFRGQSFNDVENAFCRVVDQTGKELARYKLTEQGSHTGIVISSLR
                     RNNGNWDFTALGHACRGRTIDDMHSDIVSAVIR"
BASE COUNT         1279 a         1370 c         1235 g         1236 t
ORIGIN      
        1 catgaacagg caggtatcgg gttcgccttt aaagccggat ttggcagggt actgaagatt
       61 cagcctgatc gtagattgct gaaggggtat gttttgtccg gattgtaaat tcatattaac
      121 tctcctgatt tttgattatt attaatgcgc agcgtttata tatgttccct aggcttagtt
      181 ctggacgctg gatattcggt gaggcgtaaa tatggtatga caccattttt cataacgctg
      241 aagtttctat acctgttgaa tttgaatttt cattgaccgg gtatcttatt ttccagggcc
      301 cgttccttca taagtcgcaa aagtaacata tatccgaagg gcatgctgtt gatatcagac
      361 acggaatact ggctttaacc agcaaccaca gataaacccg gggcctgcat aaaaggttca
      421 tgccagaatt aacatagctc tgctttctgc cccccccctt tcccatattc acccgttgat
      481 agcggatcga tacccaaaaa aaaccccggc attgccgggg tcagactcag cgtcttttag
      541 acattgacgc cgtgctggga agcaagcgct gacagaccac cggcaaaacc ctggccaaca
      601 gctttaaact tccactcagt accatggcga tacagttcac cgaagaccat tgcggtttcg
      661 gttgaggcgt cttcagacag atcgaaacgg gcaatttccg tcccgttgtc gttgttgtaa
      721 acgcgcatga agctgttgct caccatgccg aagttttgtt tacgcgcttc tgcatcatag
      781 atggtaacgg caaataccag ttttttgatg tctgctgaga ctttggtcag atcgattttg
      841 acctgctcat cgtcgccgtc gccttcaccg gtacggttgt cgccctggtg ctctactgcg
      901 ccatcagggc tggttttatt attgaagaaa atgaaatggg catctgacag tactttaccg
      961 tcttcaccta ctgcgaatac ggaagcgtcc agatcaaaac cctgaccatc ggttacacgg
     1021 gcatcccagc ccaggccaac catagcgaca ttcatggttg gtgcttcttt ggtcagagat
     1081 acgttgccgc cttttacgag agaaactgcc atttttagct cctgcaaaca gttgaatgag
     1141 gctgaattaa cagccccaga tatgaaaagt tacttttcga tcaggacgcg ttaatgccgt
     1201 actgagcaca tacagatgcc agaccaccag cataaccctg gcctactgcg cggaatttcc
     1261 actcaccatt gtggcgatac agctcgccga acagcatggc agtctcagtg gacgcatctt
     1321 cggtcagatc gtagcgagcg acttcagtct ggttatcgtc attaaccaga cgaataaacg
     1381 caccggatac ctgaccaaag ctctggcgac gagcctgagc atcgtggatg gtcacaacga
     1441 agatgatctt gtcaacttca gacgggacgg cgtccagttt aattttcagc gattcatcat
     1501 caccatcgcc ctcaccggtg cggttatcgc cggtgtgcgt tacggaaccg tcggatgacg
     1561 tcaggttgtt atagaagatg aaatctgaat cgccgcgcac tttgccgttt gaggccagca
     1621 ggaatgctga agcatccagg tcaaagtcct gaccgtctgt tgaacgcgca tcccagccaa
     1681 ggcccaccag gacatttttc attgacggag ctgctttact cagggagacg ttcccgcctt
     1741 tggaaagaga aacactcata aaataacctc ttcgattagt aattgttcag gttaacactt
     1801 aaggggatta actccccttt tcctcagatt caggtttgcc cgggaacatg acgcttgcga
     1861 gaatgcccag cgccagtaca cccagtacaa catacaggct ggttgttgcc gcgatgctgt
     1921 aaccatgatg ccagatgtga tcggtcgcat tcaggccgag ttttgccgcg atgaagaaca
     1981 gcagcacgat aacggccttc tccagatgaa ccaggtactg tttcagtgcc tcaaggacaa
     2041 aatacagagt acgcagaccc aggatagcaa acatcatggc actatagacg ataagcggtt
     2101 cacgactgac ggcaatgatt gccggtaccg agtcaaacgc gaacattacg tccgagagtt
     2161 caaccacagc cacacacagg aacagcgggg tcgcatacag cgccgctttt ttaccacggc
     2221 caatggtgac atcgctgttt tctggtttcg ccagttcagc atccacttcc ttctggttaa
     2281 gtaggaaggc atgccctctg agcttcggcc agatagggaa gaagcgttta accatacggt
     2341 aagccagatg ctgggagtaa tcctcaattt catcatcgtc atcaccgctt ttaagcatca
     2401 tgaccgccgt ccaggcaacg ataatagcga agacaacttc aacgtacggc cccagactca
     2461 gcaggctcgt accgatggcg acaaagatgc ccctgaagac gatggcacca atgatccccc
     2521 agtagagaac acggtggcga taacgatccg gaacggcgaa ccaggagaag attgccatca
     2581 tgacgaacag gttatcgacc gacagcactt tctccagcgc ataacccgtg acaaacagac
     2641 tggcaacctc agcaccgtgg tggatataga ggaaaccggc aaatgccatc gcaaccacaa
     2701 cccagaatac ggaccagagc gcagcactct tcagcgagat tggcttgtca tcacggtgca
     2761 taaacaggtc aataaagatg gcaccgactg aaagcgcaat aaaaacaatg accgtttcag
     2821 tcgggaagcc gatgtgtgtg gaaaccatat tttaccctta gggatgtgga tcagaggcca
     2881 aattcagccg ggtcgaagcc caacgcgata gcgatctcgc gggaggccag tttctcgtcc
     2941 tgatcgaagt taccgtcact tttcgcaacg gcaataccaa cacgtaaggc cagctgagcg
     3001 gcctcaggct gatctttcag cgccaggatg tatttcatgg tttcgccctt gccaatttca
     3061 acatcgaaat cgaagcttga aaccagttta ttgaagaact caatcacctc attggtatcg
     3121 aagaccttca gctcttctga agagcgcaga aagcccatca ttttctgctt ttcttccgaa
     3181 cttacgccgt cactcgatac ggcaatacgg gcacatacag caacggtgcc ctgcatgaat
     3241 tttttgtttt tgaaacggcc aacctggcgg gtcagttcgt cacggcctga gttaatggca
     3301 cctttaactt tgtcgaaaaa gctcatacct gtttcctttg ttgctgtgtt atttagaacc
     3361 ggcgctccag cgaaatcccc agccaaatgc ccggtccatt tcgtcctggc ctttgaagta
     3421 ctggttaatt cgctcgactt taattgcgcc gttttcgttt acgagtctgg caatggcgca
     3481 caatgtgcga cggttctcac cttcggtcag gcgggtctca atgggcggtt gatccggcac
     3541 atgaatggtg actacaccat cagtcttgtc ccagctggga acaccttcat aaataaaggc
     3601 gtaaatcagc acttcgcgga tatgcttcca ttcacgtcca ttgatatgca gccactcgcc
     3661 gtctgataca tcacccgtcc ggtcatcgcc cttgagctga acatacggtt catcgcgata
     3721 atcaccgaat gcattaccga gcgcctgaat gaccgattta tacccgtcct gaagctcaac
     3781 aaaggccccc agatccaggt cgattccttt ggagccgaac atacctgcaa agcctgattt
     3841 gctactgccc cggtgccagt tcaggttgat ccggatttca ccgaagttat cccgtttggt
     3901 caggctgatt gcaggcttct cttttgtcag cgataccttg ctcaggctga tttttgtctc
     3961 aactggcgga ggggttacaa ccggaggagt tggggctgcg ggagccggtt cgtcggcaat
     4021 atttacgccg aaatgctctg ccagcggttt aagtccaccg ttgaacccct gcgcaacgaa
     4081 gcggaatttc cagtcattat tacgacggta aaattccccc agaattaagg ctgcttcctg
     4141 acggccgctc agttcgacac tgccgctaac caggccggta gccccttgct ccacgtcgat
     4201 ggaaagatta cgaagaccgg acaccgtctg cccaccgtca caggtcacgg tgaacgcaat
     4261 tttctggaca tcaggtttca gccggttgag cgcgacagta aaggttgagt actggccttc
     4321 actgaccagg ctgacggtac catcatcgtt acgtggctga ccataaaaca ccatgtccgc
     4381 atcaccctgc accttcccgt cggcatacag gcgaaacgcc gaagcatcca cctggccgcc
     4441 cgaggtgatc cggacccgca actcctgggc tggaacgggg gcattgcccc ccggcgtcag
     4501 gttcattagc gaataaccgc tgaaacgata tcggagtgca tatcgtcaat tgtgcggccg
     4561 cgacaggcat ggccaagggc ggtaaagtcc cagttgccgt tattacggcg cagcgaagaa
     4621 ataacgatcc cggtatgtga gccctgctca gtcagcttgt agcgagccag ctctttgccg
     4681 gtttgatcga caacgcggca gaaagcgttt tcgacgtcat tgaacgactg accacggaag
     4741 ctgtttaccg taaacgccag gtattcaacg tttgcaggca ggcgggagag gttgacctta
     4801 atcacttcat cgtcaccgtc accctcaccg gtcaggttat cgccactgtg cacaacagcg
     4861 ccgcaggtgg atttcagttt gcggaaccag atggtgtcaa ttgttttacc tgtgctgtcc
     4921 attaatacac agccagcatc gagatcgatt gagtcgttgc ctccaaacaa tcctccaaga
     4981 agaccttttt tcttaaccgg atcccaaccg agaccgaaat gaagctggct taatgcagat
     5041 gattctttgc tgagtgatac cgtctggttc ttaactaatg aaaccatgtt ttattcctta
     5101 aaatggtaat gaccgagaca
//
LOCUS       DAZKIP010000212         5106 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZKIP010000212 DAZKIP010000000
VERSION     DAZKIP010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5106
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.214"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(459..1094)
                     /gene="yfdX"
                     /locus_tag="ACHOUK_004612"
     CDS             complement(459..1094)
                     /gene="yfdX"
                     /locus_tag="ACHOUK_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFP9899906.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AAERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKK
                     TNVNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVM
                     ENQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            1390..1665
                     /gene="ypdI"
                     /locus_tag="ACHOUK_004613"
     CDS             1390..1665
                     /gene="ypdI"
                     /locus_tag="ACHOUK_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFP9899907.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSNSGGGSETSYKEPEKTSAM
                     TTTHSTKFQPSQAILFKMREDAPPLNLTEETTPPFPTKANYLIHPVR"
     gene            complement(1742..1984)
                     /gene="yfdY"
                     /locus_tag="ACHOUK_004614"
     CDS             complement(1742..1984)
                     /gene="yfdY"
                     /locus_tag="ACHOUK_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFP9899908.1"
                     /translation="MFYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            2337..3257
                     /gene="lpxP"
                     /locus_tag="ACHOUK_004615"
     CDS             2337..3257
                     /gene="lpxP"
                     /locus_tag="ACHOUK_004615"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFP9899909.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            3613..3684
                     /gene="ypdK"
                     /locus_tag="ACHOUK_004616"
     CDS             3613..3684
                     /gene="ypdK"
                     /locus_tag="ACHOUK_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFP9899910.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(3749..4987)
                     /gene="alaC"
                     /locus_tag="ACHOUK_004617"
     CDS             complement(3749..4987)
                     /gene="alaC"
                     /locus_tag="ACHOUK_004617"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFP9899911.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAILESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
BASE COUNT         1332 a         1235 c         1147 g         1392 t
ORIGIN      
        1 taacgccctc tataaataat ccgcaaaata tcgtcatagc ggcactcgat atttttaaat
       61 tcctgtatca agtgctgaaa atcatttcat cgctacttcc aattcctttc tgcatggtaa
      121 gtagtgagga cgaaaagaag aatatgataa atattggttc gtggtaatca gagagagtcg
      181 cgacatttgt gatgttattc ttacaactgt aagaagcgcc ggtgatttac aagtaagggt
      241 atgattacgc tttttagagc gtaccataaa atataacata ttaaatgatt tgtgatgaca
      301 cgggattatt gcggtttact ttgaggaata taaaatgcga gtttatagaa cagcgagcgc
      361 caaataattt tatgaccaca acctgtcgta ttgtggtcat aaaaaattgt cggctggagg
      421 gagttaaatc ctttacctca atatactgcc tgtcagcatt agttcacaaa cagtgcttcg
      481 ctatcgacga tgattccatc ttccgcacct ttaagcgcca ggtttgcctc gtagtattgc
      541 tttttatcaa gcagtttctg cgcatcagca agtgcattac gagtctgttt aagcggcatc
      601 agatattggt tttccattac accaacaccg gccaggcgaa gctcttccat tgcgcccttt
      661 ttatcccctt tcgccatttt ttcattggca atttttatcg cggcttcttt ttcgggtgtc
      721 gctacataac tctcagaaat tccgacagat gcattaatga caatatattg gtcatcattg
      781 acattggtct ttttacctgg cttagcgaac tttgcccatt cggtactgtc atctgacaac
      841 aaagctgaag cttcattggt cagttctttt gccttttccg gatcgccatg aaacagagcc
      901 aggcgcgcta cctgcacgtc acgcatcgca tataaacctt gttcagagat acgctctgct
      961 gcagctgccg ttttctgggt ttgctgaact tgctgagttt gctgttgtgc cgctaccgga
     1021 gcattatcag cagcccatac agttgaagat gccagaatag ctgtgaccat cgtagccata
     1081 attaaacgtt tcatacttac ctcattcttt aacagtttta gaaaacgccg ccatcatatt
     1141 gggtgagcat gaaatgagaa atcagagagc aacggtcatt ttgaaagggt tatcttacag
     1201 ttgtagttat tatcgcaaga agtattttag tcttacaatt gtaggaatat gcttcctgtc
     1261 acagagaact attcttatgt accacaaaga gatgtcattt attatatcga tgtaaatatt
     1321 gattaattat atgtaaatac agccataaca tcgtacattc tcagtgaacc attttcagga
     1381 taactttcta tgaaagtaaa cttaatactt tttagcttat ttttattggt ctctattatg
     1441 gcatgcaatg tttttgcatt ttctaattct ggtggtggta gtgagacgag ctataaagag
     1501 ccagaaaaaa cgtcagcgat gacgacaact cactctacaa aatttcagcc atcacaggcg
     1561 attttgttta agatgagaga agatgcacca ccattaaacc tcacagaaga aaccacgccc
     1621 ccttttccga caaaggcgaa ttatcttatt catcctgtgc gatagcgtct gtcacctggc
     1681 aggtgaacaa tcatgtctga tgctgatgtg aacacctgcc caaatgaacc atcctgaccc
     1741 actatctcct gccatacccc accataaagc gaatcatcaa gccacaagcc ggggcaagaa
     1801 acataaatat ccccgtcacc agttcattac cgccagttaa ccacatggtg aagtaataaa
     1861 tgagtgcagc gaggatcacc atgccgaaga aagaagagac cgccgatacc acgttcagta
     1921 cctgaccgat gtatccgctt acgcagacaa tacacagggc gagaaacatc cataaataaa
     1981 acatagctca acctcctgcc gcgctaaaaa acctgtaact tcagtcactt taatagtaag
     2041 acaatcctta acctctggca atttttgcta agcgtcaatg aaaacagcat gttaaatgca
     2101 atactattgc gtgcggaaaa ttttttactt tgcacgatta ataatcagta gctgaaagca
     2161 gtcagccagt gataaagtac agataccgca ttcatgctcc tcgtggttat gtcctgacta
     2221 gtctttacac tctttacagg aaccattgtc gtacatgatg gcccaaccaa ttgagtgtac
     2281 cgctgtcgac atggaatctt ctgcgataca acaattcgta tctacagaag gtaactatgt
     2341 ttccacaatg caaattttcc cgcgagtttc tacatcctcg ctactggctc acatggtttg
     2401 ggcttggtgt actctggctt tgggtacagc ttccttatcc tgttctctgc tttctcggca
     2461 cgcgtattgg cgcaatggcg cgaccattcc tgaaacggcg tgaatctatc gcccgtaaaa
     2521 acctggaact ttgcttcccg cagcattctg cggaagaacg cgagaagatg attgccgaaa
     2581 actttcgttc actcggcatg gctctggtag aaaccggcat ggcgtggttc tggcccgaca
     2641 gtcgcgtacg taaatggttt gatgttgaag ggctggataa ccttaaacgc gcacaaatgc
     2701 aaaatcgcgg cgtaatggtt gtcggcgtcc attttatgtc gctggaactg ggcggccgcg
     2761 tgatgggact ttgtcagcca atgatggcta cctatcgtcc acataataat cagctgatgg
     2821 aatgggtgca gacccgtggg cgcatgcgct ctaacaaagc gatgatcggc agaaataatc
     2881 tgcgcggcat tgtcggtgca ctgaagaaag gtgaagcggt atggtttgct cccgatcagg
     2941 attatggtcg taaaggcagc tccttcgcgc cgttctttgc ggtggaaaat gtcgccacaa
     3001 ccaatggcac ctatgttctc tcccgtctct ctggcgcagc catgttgacc gtaacgatgg
     3061 taagaaaagc ggattacagc ggatatcgtt tgttcatcac cccagagatg gaaggctacc
     3121 cgacagatga aaatcaagcc gctgcctata tgaacaagat tatcgagaaa gagatcatgc
     3181 gcgcaccgga gcagtacctc tggatccacc gtcgctttaa aacgcgcccg gtgggagaat
     3241 cgtcgttgta catttaacaa actgctctta ctctttccct cctcaaatcg gccataataa
     3301 cctcatggtt tttatggctt ttttcttaag attttttcct tcgctgaata tctaattatt
     3361 accgccagaa attaaactgt cgccgatatg agacacctgt aagttactaa aagtaaataa
     3421 gaaaatgcct cttgtctcgt ttcagtttta ggcgtacatt agcgccgtct cgagcagttt
     3481 agctcagaat tctggggtga tgcccacggt aaaatacagg acttttaagg tcgttttgcc
     3541 cggtgtcaac tcactgtatc aggtgtgatg aagtcattca ggcgtaaccg taattacgcg
     3601 gagagatgta aagtgaaata tttctttatg ggcatttctt ttatggtcat cgtttgggcc
     3661 ggtacttttg ccctgatgat ctaaaagcaa actgtgcaat gaaaaacagg agccatcagg
     3721 ctcctgtttg catttctgac gacggtgttt attccgcgtt ttcgtgaata tgtttgctgc
     3781 tggcgggtag taaaccgtcg gcacggaaca tcgctttaat cccacgaatc gcctgacgaa
     3841 tacggtcgcg gttttcaatc agtgcaaagc gaacatgcgt gtcgccgtag tcaccaaagc
     3901 caatccctgg agagacacag accttcgctt cgttaaggag cttcttggca aattccagcg
     3961 atcccatggc cgcatatggt tccgggattt tcgcccagac atacatcgaa gccttcggca
     4021 tttcgaccat ccagcccgct tcatgcagcc ctttaaccaa tacatcgcgg cggcgtttgt
     4081 actgttcggc aatgtcgcgc acgcactgtt gatcgccctc cagcgccgca atcgccgcca
     4141 cctgcaacgg cgtaaaagtg ccgtaatcgt gatagctttt gattcgcgcc agggcactga
     4201 ccagcgtttt gttgccgacc ataaagccga tacgccagcc cgccatgttg tagcttttcg
     4261 acagcgtaaa gaactcgact gccacatcgc gtgcacccgg tacctgcatg attgacggcg
     4321 ctttccagcc atcgtagacg atatcggcat aggccaggtc atggaccacc agcacatcgt
     4381 agcgtttcgc cagcgccact actttttcga agaactccag ttccacgcat tgcgcggttg
     4441 ggtttgacgg gaagccgagg atcatcatct tcggtttcgg ataactttca agaatggcgc
     4501 gctccagttc gttgaagaaa tcgacacctt ccaccagcgg cactgagcgt acctgcgccc
     4561 cggcaatcac cgcgccataa atatggatcg ggtagcttgg attcggcacc agcaccgtgt
     4621 caccatgatc cagcgtcgcc agcatcaggt gtgccagccc ttctttcgaa ccaatggtga
     4681 cgatggcttc tgattccgga tcgatttcaa cgtcgtagcg atcctgatac cagcgggaaa
     4741 tggcgcgacg taaccgcgga atgccgcgtg aagtggagta accatgcgtg tccggacgct
     4801 gggccacagt acataatttt tcgacgatat gcggcggagt cgcaccgtcc gggttaccca
     4861 tgctgaaatc gataatatct tcgccgcgcc gacgcgcagc cattttcagt tcagcggtga
     4921 tgttaaaaac gtagggcggg agacgatcaa tgcgcgtaaa gcgacgttca gggcgagtgt
     4981 cagccataat ttcctcagat taacgttagc gcccggaccg tccgagcgac gctgccacga
     5041 tggtggctgt tttgaaaata gcctgattaa tttctgtctg tccagcgttt tagaacattt
     5101 ttatta
//
LOCUS       DAZKIP010000213         5067 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZKIP010000213 DAZKIP010000000
VERSION     DAZKIP010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5067
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.215"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            2..1048
                     /gene="tsuA"
                     /locus_tag="ACHOUK_004618"
     CDS             2..1048
                     /gene="tsuA"
                     /locus_tag="ACHOUK_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001647169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFP9899912.1"
                     /translation="MLSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYALLIA
                     ISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRAGEGL
                     IGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVAVLLVIT
                     LWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLSEATG
                     RMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGAFIAAKASREFRVRAADAQ
                     TTVRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWVGLVFMILGVWTASWLVYV
                     RPQRKARLATAAAN"
     gene            1062..1289
                     /gene="tsuB"
                     /locus_tag="ACHOUK_004619"
     CDS             1062..1289
                     /gene="tsuB"
                     /locus_tag="ACHOUK_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFP9899913.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1332..2756)
                     /gene="sbcB"
                     /locus_tag="ACHOUK_004620"
     CDS             complement(1332..2756)
                     /gene="sbcB"
                     /locus_tag="ACHOUK_004620"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFP9899914.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSD
                     TLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQMLA
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            2938..4116
                     /locus_tag="ACHOUK_004621"
     CDS             2938..4116
                     /locus_tag="ACHOUK_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9899915.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     DQIKPFLEKAMTTYNDVLFPGEDINKPISIAAANRFVNRIRGGMDLGYWRTHDFRRTL
                     VTRLSEMNVEPHVTERMLGHELGGIMSVYNKHDWIEAQRKAYELHADKLFWHIRSISD
                     "
     gene            complement(4097..4288)
                     /locus_tag="ACHOUK_004622"
     CDS             complement(4097..4288)
                     /locus_tag="ACHOUK_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9899916.1"
                     /translation="MTDTSLIPEKEVMNKLGVSSRQTIWNYTKRHGFPKPVRTHPKSY
                     LREAVEGWILNGGVNQKCS"
     gene            complement(4366..4710)
                     /locus_tag="ACHOUK_004623"
     CDS             complement(4366..4710)
                     /locus_tag="ACHOUK_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899917.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHGFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKIHGNKAP
                     ETYRRISELRKD"
BASE COUNT         1245 a         1202 c         1278 g         1342 t
ORIGIN      
        1 gatattaagc gggctaattt gtggtgctct gctgggattt gtcatgcagc gtgggcgttt
       61 ttgcctgact ggtggttttc gcgatatgta tatcgcgaaa aataatcgca tgttttacgc
      121 cttgctgatt gcaatttcgg tacaaagcgt gggggttttt gcgttaattc aggcgggcct
      181 actgacttac gaagccgggg cgttcccgtg gcttggtact gttataggtg ggtatctctt
      241 cgggctggga attgttctgg cgggtggatg tgccaccggg acctggtatc gcgcgggtga
      301 gggattgatc ggcagttgga tcgcgctttt cacttatatg gtgatgagtg cggtgatgcg
      361 ttctccacac gccagtggtt taaatcaaac cttgcagcac tacactactg aacataactc
      421 tattgctgag acttttaatt tgtctgtgtg gccgttggtt gccgttttgc tggtgataac
      481 gctctgggtg gtgatgaaag agttgaagaa gccaaaactc aaagtcgcga ccttaccgcc
      541 gcgccgaacc gggatcgctc atattctgtt tgaaaaacgc tggcatccct ttgtcacagc
      601 tgtactcatc ggtttgattg cgcttttagc ctggcccctg agtgaagcaa ccggacgtat
      661 gtttgggtta ggcatcactt ccccaacggc caatattctg caatttctgg tcgcgggtga
      721 cgtgaaatac attaactggg gcgttttcct ggtgttaggg atcttcgtgg gggcatttat
      781 tgcagccaaa gcgagccgtg agttccgcgt tcgcgcagct gatgcacaaa caacagtacg
      841 tagcgggcta ggtggtgtac tgatgggctt cggtgccagt attgcaggtg gttgctctat
      901 cggtaatgga ctggttatga ctgcaatgat gacctggcag ggctgggttg gccttgtatt
      961 tatgattctc ggagtctgga ctgcgtcctg gcttgtgtat gttcgaccgc agcgtaaggc
     1021 gcgactggca actgctgcgg caaattaagt aaggtgtaaa gatggcgatt aaaaagctgg
     1081 atgtggtgac gcaggtctgt ccgtttccac tcatagaagc aaaagcagca ctggcagaaa
     1141 tggccagcgg cgatgagttg gtaattgagt ttgattgcac ccaggcaact gaagcaatcc
     1201 cacagtgggc ggcagaagaa gggcatgcca tcaccgatta tcagcagatt ggcgatgcag
     1261 cgtggagcat caccgttcaa aaagcctgac tcaacattgt cctccgccgt accagcggcg
     1321 gaggcttcac attagacaat ctcttccgcg tactgccaaa gcgcttttaa cagcgccact
     1381 ttttctttat cgtcggcata ctgctgcgcc agcatctgaa tctcttctgc atagccttgt
     1441 aaaaactctg gcgtgaagac ctggcggcgg tgctccagcc agcgttgctg ctcggcataa
     1501 tccagcgtcc ccgggaagtt gcgtgcccga taattgaaca acagcttttc aatccgttta
     1561 tcaacaaaag tgatatccag tgccggtaaa ttacgcggct cggtttcaag cacaattttc
     1621 attgctgcac gatcggcgtc actgaaaaag ccattataaa gctgtgcatc cacgttatct
     1681 gaaggcgtaa acggttcggc ttccgcgaat atcgccacca ctttttcacg cacttgcgga
     1741 ttttcacgca gaattttcag gttatcgagg caatgctgac gattaattcc cagtcggtcg
     1801 gcatcttccg ggcgtagcgt attcgcctgg gccagcaccg gacatttatt gatatgtacc
     1861 agtttaaccg gaacggcggc gttatcgcca agatcggttt ttgcggtata taaacgctcg
     1921 cgcaatgtgt cgctatccag ttccagtaat ggcgaaatat ctcctgccaa atccaccata
     1981 attacggcat tgcgattttc aggatgccac gccagcggtg ccacccagct ggtattgccg
     2041 cgccatgccc caaacattcc ggaaacgtga accaggggtt tcatctgcgg aacatcaatc
     2101 aacgccatca gtttgtgttt attacgatgg gtaaagagat aatcaaacag gcgtggttga
     2161 cgcgttttta ccagtttcgc catcgcaata gtggcgtaca catcagccat cgcatcgtgg
     2221 gcgttgctat gttcaatacc attcgctttg gttaaatgct caaggcgaaa gctcggtaga
     2281 ccgtcatcat tttcaggcca gtttattcct tccgggcgca gggcatagca ggcacgcata
     2341 acatccagta aatcccagcg cgagttatca tgctgccagc tccaggcgta aggatcgtag
     2401 aaattacgat aaaaaacgtt gcgtgtgact tcgtcgtcga aacgcacatt gttgtagccc
     2461 agaatacagg tcttcggtac ggtaaaaagc gagtgaatac gggcggcaaa tgcggcttcg
     2521 ttttctcctt tcgcccgagc ttcctgcggg gtaataccgg taattaatac tgctccaggc
     2581 tggggtaaat agtcatccgc gggcttgcag taaaagactt cgggttcgcc gatgacattg
     2641 aattcgtcat cggtgcgaat ggctgcgaac tgtgcagggc gatctaacgc ggggtgcgtg
     2701 ccaaaggttt cgtaatcgtg aaagaggaag gtaggttgct tatctgtgtc tgtcattgtc
     2761 tagcattgtc caatgtagtt aattaactgt ctgttataac tcgaaaaaca accctttttt
     2821 cacctgagta tgtctacgaa cgtcaaatat agttgtttac tgtgcgctgt tgtccatgtg
     2881 atattgtgta ccagatgtgt accaaaccat tatttctgag cgtaccaaat attatttatg
     2941 gctataagcg acacaaaact gcgtactatt tatggtaaac catattctgg cccacaagaa
     3001 gtggctgatg ccgatggcct cagcgtacga atttcaccga agggggtcat ccagttccag
     3061 taccgctatc gctggcatgg caagcctaat cgacttgggc ttggtcgata cccatccctg
     3121 tctttgaagg atgccagaca gatcactgct gacttgcgaa agctctattt ctcaggaacg
     3181 gatccacgca cttattttga agagaaggtg gagaactcca tgacggtcgc ccagtgtctc
     3241 gactactggt tcgacaacta cgtctctaca actctcagag aaaagaccca agcactttac
     3301 cgatcaacgg ttatgaagcg catgcatgac gccttcccta atcgtccggc atcttctatc
     3361 acggttaagc aatgggttga cctgcttacc gaagaagaaa aagataatcc acgccgagca
     3421 aggcaggtgc taagtcaact aagatcagca attagttggt gcatgcggcg tcagttgata
     3481 gatagttgcg caattatgag catccaacca agggacttcg gctcccgcgc tgaggtaggg
     3541 gatcgggtac tgtcgtatca cgaactggct aagatctggc ttgctattga aagaagccgt
     3601 gcgtctacgt caaataagct ccttcatcag atgcttatgc tgtggggggc gaggctctca
     3661 gagcttaggc tggcaacaaa gacagaattt gacctgctgg acaacgtatg gaccgtaccg
     3721 aaagagcata gcaagatggg taatgttatc cgccgtccaa tcttcgatca aattaagcct
     3781 tttctcgaaa aggccatgac aacgtacaat gatgttcttt tccctggaga agacataaac
     3841 aaaccgatca gcatcgctgc agctaaccga ttcgtaaata gaataagggg agggatggat
     3901 ctaggttact ggcgaacaca tgatttcaga agaacgcttg ttacacgtct gtccgagatg
     3961 aatgtcgagc ctcatgttac tgagcgaatg ctcggtcatg aacttggcgg gataatgtcc
     4021 gtatacaata aacacgactg gatagaggct cagcgcaaag cgtatgagct tcacgctgat
     4081 aaattgttct ggcacatcag gagcatttct gattaacgcc accgttaaga atccaccctt
     4141 caacagcttc acgaaggtat gatttggggt gggttctgac tggcttcgga aatccgtgcc
     4201 gtttggtata gttccagatt gtctgacgtg atgaaacacc gagcttgttc atcacttctt
     4261 tctcaggaat caggctggta tcggtcatct taattctcca ggcaaaaaga aaccgccata
     4321 tagcggctct atcagatatg aacaggcctc atcgagtgtg aggcgttagt ccttgcgtag
     4381 ctcgctgatt cttctgtaag tctctggtgc tttgtttccg tgtatcttca tttcagactt
     4441 caacagagca acgagggaat cccattcgtt gaggattcct ttgaatgccg gaacgcgctt
     4501 tgcaaccttg tcgaatgaat ctctgatttc tggaatctgc tcaacaagtg caacgcatcg
     4561 tctgaaatcg gctgcgtcat gtggagcacc gaagccatga ccatagatat tctttttcag
     4621 gccacatgcg attgaggcaa gagttgcgct actgatgccg acatcgccag ttgattgcca
     4681 tttcaaaacc ttcatagcca aatctgacat ttcttgtctc cataaaacaa aactcgccgt
     4741 agcgagttca gataaaagaa atccccgcga gtgcgaggat tgttagttgc gctctgctgc
     4801 tgccttagcc attaccatat ccacacaatt tcaccagaca tgattctcgc aatcactatc
     4861 atcaccaaag tgataatcac aactttaact ggcggcatca ttcaccatcc tgctgcggtg
     4921 ctgctggata tacttttctc atccaatgcg tggcgtcatg aataacgaac tgatattcat
     4981 catcagtaaa catcgagcca tcccactgta gcccgtcaag aacacctctg gatggagaga
     5041 atacccagat atcgaaatcg tatttct
//
LOCUS       DAZKIP010000214         4961 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZKIP010000214 DAZKIP010000000
VERSION     DAZKIP010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4961
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.216"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(31..1533)
                     /locus_tag="ACHOUK_004624"
     CDS             complement(31..1533)
                     /locus_tag="ACHOUK_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFP9899918.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIDVNDWQPHANPVVVWDIFG
                     EKGHSVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(1663..2682)
                     /gene="ahr"
                     /locus_tag="ACHOUK_004625"
     CDS             complement(1663..2682)
                     /gene="ahr"
                     /locus_tag="ACHOUK_004625"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFP9899919.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELRPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLPVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIKHVRDGKARYRVV
                     LKADF"
     gene            2878..2962
                     /locus_tag="ACHOUK_004626"
     tRNA            2878..2962
                     /locus_tag="ACHOUK_004626"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2912..2914,aa:Leu,seq:caa)
     gene            3053..>4961
                     /locus_tag="ACHOUK_004627"
     CDS             3053..>4961
                     /locus_tag="ACHOUK_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6538 domain-containing protein"
                     /protein_id="HFP9899920.1"
                     /translation="MSQRYKLYRRTSGIYVVRISVPQRFRRYAGQCEIHTSTGTHDLH
                     EAKQKSALLLAVWYQTLQEYEQLDYRTLSDCAPLLAGEGMISLSNFAQSIELPISQLI
                     REVVNRNLPVFWLATGQFGFYVDEFNAVEREPGAKREKQSDDEKDQPKEVIILNSAFE
                     LGIESFANGYLRPFNPRHTLDCLLSAGVSEGEAAFRTSGDNQSGGWFFDLPGVDITAD
                     SLLISKVHAEGLRLTWLVKTTPPAVSIHPAVPLVAPVIANEYVHRKHYNENLSWLREE
                     YLKHRRKGKVSEAALRDIRYYFDLMIEVMGDIQLEDFDRDFLRAYESKLRTIPANRNL
                     MKGKHGVKTLDELIAKAAECGDKLMTEESVKKYINGLYGAMEWAVDDGKFLKSPCDNF
                     FPPDDKGEREQDHTDIFEPHEIKAIFSQPWFVAGTVERNAQGRFHQYCPFHYWAPLLG
                     LMTGARVNEIAQLMLDDVLADDGVYYLNLESDSENGKKLKNANSRRKIPVHSTLIELG
                     FIEYVDALKAAGYDRLFPELKPHKTKGYGRPVSAWFNESLLAGRLKLERDRSKSFHSF
                     RHSVSTLLKEKGVSSELRGQLLGHVRGKTETEVRYSKDLKPVHMVEVVEKIDFSLPEI
                     ARFNIPDGLDAV"
BASE COUNT         1220 a         1309 c         1206 g         1226 t
ORIGIN      
        1 cgcatcaggc aaagacaaat ctcgcctgac ttaccttctt ctccccccca gcaaactccc
       61 caacatcccg cgtacaatct gattcgtcac ttggcgagcg gcgcttttgg ccattgtttg
      121 caccacacca tctttcttcc cgccgcgtgg tccggtagtg ccgaacaaaa tatccttcaa
      181 tccaccaaga atgccgtcat ccaccgctac ctctttccct ttcgcggggg gattattttg
      241 ctgctcggta ctggcctgaa agcctttttg caacatctca taggcggatt cccggtccac
      301 ctcatcctca tatttgccat acaccggaga gtgattaatc aagccattac gctcatcttc
      361 cgtcaccggc cccatccgcg aacaaggcgc gatcaccatc gcacgctcca ccacagaagg
      421 gcttcctttc gcatccagaa aagagatcaa cgcctcgccg gtgcccagtt cctgaatcgc
      481 cttttcggta tcaaacgccg gattggcccg catggtttgc gccgcagctt ttactgcttt
      541 ctgatctttg ggcgtaaaag cccgcaaagc gtgctgaacg cgattaccga gctggccgag
      601 cacattatcc ggaatatcag acgggttttg cgaaacgaac cagacgccca cgccttttga
      661 gcgaataagc cttatcacct gctcaatctt atccagcagt acctgcggtg catcgttaaa
      721 cagcagatgt gcttcgtcga agaaaaacac cagttttggt ttctccagat cgcccgcttc
      781 cggcaattgt tcatacaact ctgagagcat ccacagcagg ctggcggcgt acagtttcgg
      841 catctgataa agtttctcgg cgctgaggat attgataacg cctttaccgt tggcatcggt
      901 gcgcatccag tctttgatat ccagcatcgg ctcaccaaag aagtgtgctg cgccttgctg
      961 ttccagcgac aacagcccgc gctggatggc accaaccgat gcgctactga tatttccgta
     1021 ctgattctgg aaagatttgg cgttatcgcc gatgtactgg gtaatcgccc gcagatcttt
     1081 aaagtcgagc agtaacagcc cctgatcgtc agcaatgcgg aagatgatat tcagcacgcc
     1141 agattgcaca tcgttgagat tcaacagccg cgccagcaac agtggcccca ggtctgaaac
     1201 cgtcgcccgt accgaatggc ctttctcgcc aaagatatcc cacaccacca ccggattagc
     1261 atgcggttgc cagtcattga cgtcgatatt tttaagcctt gcgagcagtt tttccgacgc
     1321 cgttcctgcc tgcgcgatac cggtcagatc gcctttcaca tcagccataa acaccggcac
     1381 gccgatttcc gacaatgact ccgccagttt ttgcagcgtg acagttttac ccgtccccgt
     1441 tgcgccagta atcagcccgt gacggttagc cattcccggc agtaaaaata gttctgtgtc
     1501 cggcgtgcgg gcaattaaca ggggttcgct catgagatat cctccagttt cctgcctgga
     1561 gtataggcaa cggctgcgca ctcttcaccg ctaaattcag cagattgcga gacgccttcc
     1621 aaagagattt aagtgccggg cgacaggcac ccggcaaaga gatcagaagt cagctttcag
     1681 aaccacgcgg taacgcgcct taccgtcgcg cacatgcttg atggcatcgt taattttcga
     1741 catcgggaac aattcggtag tcggcgcaac cttgctgcgg gcggcaaagc gcatcagctt
     1801 acgcaactca taaggtgtac cggtagcaga gccagagaca ctgcgatcgc ccgcaatcag
     1861 cgtaaaggcc ggaaccggca acggcgtgag aaccgcaccg accgtatgga aattaccgcc
     1921 ataggtcagc gcctcaaaat aaggctgcca gtcgaggctg acgttcacag tattgataat
     1981 gagatcaaac tgccccgaca gggctttcag tgcctgcgga tcgcggctat tcaccacttt
     2041 atcggcaccc atcgccagta cttcctgctc tttcgccgga ttagaactaa aggccgtcac
     2101 ctcgcatccc attgcgtgca gaagttttat agcgatatgc cccagcccgc caataccaat
     2161 taccccaacg cggctggtag cagtgatatg gtgcatcaac agtggtttaa agaccgtgat
     2221 accgccgcac aacagcggcc cggcggactc gatatcaata ttttctggca gtggaatcac
     2281 ccattgccag tccgcacgca acttctcggc aaagccaccg cgattcataa tcgtcggcac
     2341 agcaccttgc tcgcagttga tctgattacc gctaatacag gcgtcgcagt gaccacagct
     2401 acgcgccgtc cagccaatcc cgacacgctg gccgacctgc aaacctttat cctgcgcggc
     2461 gctcccgagc gccaccacgc gcccaatcac ctcatgcccg gcaaccagcg gatattgtga
     2521 aaatccccat tcattgtcga tcatcgatag atcggaatgg cagatcccgc aataatccac
     2581 ctgcacttca acatcttgtg gcctcagctc accgggatcg tactcataaa cttccagttc
     2641 gccgcccgct tcttttgcgg catagctttt tatcatcgac attttttggt ccttctctgg
     2701 tgttgtttgg gaagtgtaga gcatggcagg gcggggtgcc tggagtgtga caaaggttac
     2761 acatcgctgt atgcaatgct gaaaatttca gcacttagcg aggtgcgagc aagctggcgc
     2821 ttgcatggtg gcgtgcgaca ggtataatcc acaacgtttt ccgcatacct cttcagtgcc
     2881 gaagtggcga aatcggtaga cgcagttgat tcaaaatcaa ccgtagaaat acgtgccggt
     2941 tcgagtccgg ccttcggcac caagactcat ctaaactatt gttttaaaac aatttaattc
     3001 ataatcttgc ttgaaatcgc gtagcccctc ttacaaaagg gtatacgagg cgatgtctca
     3061 gcgatataaa ctctatcgtc gaaccagcgg catttatgtt gtccgcatta gcgttcccca
     3121 acgttttcgt cgatacgcag gacagtgtga gattcacact tcaacaggca ctcacgatct
     3181 tcatgaagca aagcagaagt ccgcgctcct gttggcggtc tggtatcaga ccttacaaga
     3241 gtatgaacaa ttggattatc gaactttaag tgactgcgcc ccactgcttg ctggtgaggg
     3301 tatgatctcg ctttctaact ttgctcagtc aatcgagttg cctatatcgc aattgattcg
     3361 agaggtggtt aatcgtaacc tcccggtatt ctggctggcg actggtcagt tcggtttcta
     3421 tgttgatgaa tttaatgcag tagagcggga accgggtgca aaacgagaaa aacagtctga
     3481 tgatgaaaag gatcaaccta aagaagtcat cattctcaat agcgcgtttg agctgggtat
     3541 cgagagcttc gcaaatggtt atctccgccc cttcaatccc cggcatactt tagattgtct
     3601 gttgagcgct ggagtatccg aaggagaggc tgcatttcga actagtggtg ataaccaaag
     3661 tggaggttgg ttcttcgatt tacccggcgt agatataact gctgatagcc tgttgattag
     3721 caaagttcat gctgaaggcc ttcgacttac atggctggtt aagaccacgc caccagcagt
     3781 tagcattcac cctgccgtgc ctcttgtcgc tcctgttatc gctaatgaat atgttcaccg
     3841 caaacattac aatgaaaact tgtcatggct tcgtgaagag tatttgaaac atcgacgtaa
     3901 gggcaaggta tcagaagcgg cgctccgcga tattcgctat tacttcgatt tgatgattga
     3961 agtgatgggg gatattcagt tggaagattt cgaccgtgat ttcctccggg cttatgagag
     4021 caagttgcgc acaattcctg ctaaccgtaa tttgatgaaa ggtaagcacg gggttaagac
     4081 gctggatgag ttaatcgcca aagcggcaga atgtggcgat aaactgatga cagaagagtc
     4141 tgtcaaaaag tatatcaacg gcctttatgg tgcaatggag tgggctgttg atgatggtaa
     4201 gtttctgaaa tcgccatgcg acaacttttt ccctcccgat gacaaaggtg agcgagagca
     4261 ggatcacact gacatatttg aaccgcatga aattaaggca attttttcgc aaccgtggtt
     4321 tgtcgctgga actgttgaac gtaatgcgca agggcgattc catcaatatt gcccgtttca
     4381 ctattgggcg ccgttgttgg gcttgatgac gggggcaagg gttaacgaga ttgcacagtt
     4441 aatgctggac gatgttctgg cagatgacgg cgtttattac ctgaaccttg aaagcgatag
     4501 cgaaaacgga aagaaactaa aaaacgccaa ttcccgccgc aagattccgg ttcattctac
     4561 gctgattgaa ctcggtttta tcgagtatgt ggatgcgttg aaagctgccg ggtatgaccg
     4621 tctttttccc gagcttaaac cacataaaac caaaggctat ggtaggccgg tttccgcatg
     4681 gttcaatgaa tcattgcttg cgggtcgatt aaaacttgaa agagacagaa gcaaatcttt
     4741 ccactctttc cggcattctg tttcaacttt gcttaaagag aagggtgtta gttcggaact
     4801 gcgtgggcag ctacttgggc atgtgcgagg caaaacagaa actgaagtgc gatacagcaa
     4861 agatttaaaa ccggttcaca tggttgaggt tgtcgaaaag attgattttt ctttgcccga
     4921 gatagcgaga ttcaacattc ctgatgggct ggatgctgta a
//
LOCUS       DAZKIP010000215         4852 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZKIP010000215 DAZKIP010000000
VERSION     DAZKIP010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4852
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.217"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            220..762
                     /locus_tag="ACHOUK_004628"
     CDS             220..762
                     /locus_tag="ACHOUK_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFP9899921.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            862..2064
                     /locus_tag="ACHOUK_004629"
     CDS             862..2064
                     /locus_tag="ACHOUK_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFP9899922.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTLALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            2284..3066
                     /locus_tag="ACHOUK_004630"
     CDS             2284..3066
                     /locus_tag="ACHOUK_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFP9899923.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCNAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTKLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPATVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            3081..4281
                     /gene="rhmD"
                     /locus_tag="ACHOUK_004631"
                     /pseudo
     CDS             3081..4281
                     /gene="rhmD"
                     /locus_tag="ACHOUK_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
     gene            4338..>4852
                     /locus_tag="ACHOUK_004632"
     CDS             4338..>4852
                     /locus_tag="ACHOUK_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9899924.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMG"
BASE COUNT         1114 a         1179 c         1318 g         1241 t
ORIGIN      
        1 aaagtgacag taatctcttt tgcgatgata actgcactta ttattatttt tgtgtgtatc
       61 ggtaaactgt gtttattttt tcggcgttta attatttaag aagtatttag ttacaataag
      121 tgactagatc ccttttttcg ctcattgaat cctctgcgcc aggcagtaca atcccgcagc
      181 tttgggaatt ttgattaatt gcttgtggga aaggggataa tgaaaaaaat tgcgctagca
      241 ggtctggccg gaatgctgtt ggtttctgca tcggtcaatg caatgagcat cagcggccag
      301 gcgggtaaag aatacaccaa tattggtgtc ggttttggga ctgaatcgac gggcctggct
      361 ttaagcggta actggacaca taacgacgac gacggtgacg tcgcgggcgt ggggctgggg
      421 ttgaatctgc ctctcgggcc gttaatggcg accgttggcg gaaaaggcgt ttacaccaac
      481 ccgaattacg gcgatgaagg ctatgccgca gcggtaggag gtggtttgca gtggaaaatt
      541 ggcaacagct tccgtttgtt tggcgagtat tactactctc cggattcact ctccagcggt
      601 attaaaagtt atgaggaagc gaatgctggc gcgcgttaca ccattatgcg tccagtcagt
      661 attgaggcgg gttatcgcta cctgaatctg tcgggtaaag acggtaaccg cgacaacgcc
      721 gtggctgacg gcccgtatgt tggggttaac gccagtttct gatcccttcc ggcgcggcat
      781 tctgtcgcgc cattcagttt cttactacgc cttatctccc tttgcgttat agtgattcga
      841 taacaaaagc aggagataac catgttaaaa gtggaaatgc tatccaccgg ggatgaagtg
      901 ttacacgggc aaatcgttga cactaacgct gcctggctgg ccgatttttt ctttcatcag
      961 gggttgccat tatctcgccg caatacggtg ggggataacc ttgatgactt agtcaccatt
     1021 cttcgcgaac gtagtcagca cgccgatgtg ctgatcgtta acggcgggct gggaccgacc
     1081 agcgatgatt taagcgcact cgccgctgcg acagcaaaag gtgaaggcct ggtgctgcat
     1141 gaagcctggc tcaaagagat ggaacgctat ttccacgaac gtggacgagt aatggcaccg
     1201 agcaaccgta aacaagcgga gctgcctgcc agtgctgaat ttatcaataa cccggtaggc
     1261 accgcctgtg gttttgccgt gcagcttaat cgttgcctga tgttctttac tcccggcgta
     1321 ccgtcagaat ttaaggtgat ggtcgagcac gaaatcctgc cgcgtctgcg cgagcgtttt
     1381 tctttaccac agccgccggt ttgtctgcgt ttaactacct ttggtcgttc ggaaagcgat
     1441 ctggcacaaa gcctggacac tctacaactg ccgccgggcg taacaatggg ctatcgctcc
     1501 tcaatgccga tcatcgaact gaaactcacc ggaccggcaa gcgagcaaca ggcgatggaa
     1561 aaactgtggc tggacgttaa acgtgttgcc ggacagagcg tgattttcga aggcaccgaa
     1621 ggactgcccg cgcagatcag tcgcgaattg caaaaccgcc agttcagcct gacgttgagc
     1681 gagcaattca ccggtggttt attggctttg caactttctc gcgcaggtgc tccactgctg
     1741 gcgtgtgaag tggttccttc acaggaagaa accctggcgc aaactgcgca ctggattaca
     1801 gaacggcgag ccaaccattt tgccgggctg gcactggctg tttcgggttt cgagaacgag
     1861 catctcaact ttgcgctagc cacgccagat ggcactttag ctctgcgtgt gcgtttcagc
     1921 actacacgct atagcctggc tatccgtcag gaagtgtgcg caatgatggc actgaatatg
     1981 ctacgccgtt ggttaaacgg ccaggacatt gccagtgagc atggctggat tgaggttgtt
     2041 gagtccatga ccttatccgt ctgattccgc cactctgtgc cggtttactg caccggcaca
     2101 ttccccctcc ggcaaaactt gtccaacctg ttgtactcaa taagaaaaaa tccgtgcgct
     2161 gattcacaaa tttgtgcgta atttctccag tccgttggac gaggagcggg atttgatgcg
     2221 cttcacaatt ttactccccc tgagcaacga gactgattcc agtatctaag actggagtcc
     2281 agtatgttgg aatcaagcaa agttcccgcc ctgacacggg ctattgatat tctcaatttg
     2341 attgcccgca ttggcccctg taatgctgcg acgatcattg acacattggg gatccctaaa
     2401 agcacggcct atttgctgct taatgaactc aggcgtcagc gttttcttag ccttgatcac
     2461 caggaaaatt tttgtttgtg gaccaagctg gtggagcttt ccggtcatgc gttaagcaag
     2521 atggatctcc gggaactggc tcgaccgcgt ttgacgcaac tgatggatac caccgggctg
     2581 ttatgtcacc tggggattat tgataacggc agcgcttatt acattttgaa agtggagtca
     2641 tcagccacta tcagcgtgcg ttcacatgaa ggaaaaagcc tttcgcttta tcgctccgga
     2701 ataggtaaat gcctgcttgc ctggcaacct gcaactgtac agcaaagtat tatcgaaggg
     2761 ctggtctggg agcaggcgac acccacaacg attactcatc cacagcaact tcacgaagaa
     2821 ctggcgcgta ttcgccgtca gggctggagc tatgacaacg gcgaagatta cgccgatgtc
     2881 cgttgtgtcg ctgcgccggt gtttaacgcc aataacgagc ttaccgctgc gatctcggtg
     2941 gtcggtaccc gcttacaaat caatgaagaa taccgtgatt atctggcggg caaggccatt
     3001 gcctgcgcca gggatatttc acgtctgttg ggatggaaaa gtccctttga cttacaagcc
     3061 tcataacaat ggagaacatc atgaccctac caaaaattaa acaggttcgc gcctggttta
     3121 ctggcggtgc gacagcagaa aaaggcgctg gcggcggtga ttatcacgac cagggggcga
     3181 atcactggat agacgatcat attgccaccc cgatgagtaa ataccgcgat tacgagcagt
     3241 tacgccagtc atttggcatt aacgttcttg gcacgttggt tgttgaagtc gaagcagaaa
     3301 acggccagac cggattcgct gtttcgacag ccggtgaaat gggctgtttt atcgtcgaaa
     3361 aacaccttaa ccgtttcatt gagggtaaat gtgtcagtga tatcaaactg atccatgatc
     3421 aaatgctcaa tgccaccctg tattactccg gctctggtgg cctggtgatg aacacgattt
     3481 cctgtgtcga tctggctctg tgggatctgt tcggcaaagt ggtcgggctt ccggtttata
     3541 aacttttagg cggcgctgtt cgtgatgaga ttcagttcta cgccacaggt gcgcgtccgg
     3601 atctggcaaa agagatgggc tttatcggtg gcaaaatgcc gacgcactgg gggccacatg
     3661 atggcgatgc ggggatccgc aaagatgccg ctatggtcgc ggatatgcgt gaaaaatgcg
     3721 gtgaggattt ctggttaatg ctcgactgct ggatgagtca ggacgtgaac tatgcgacca
     3781 aactggccca cgcttgcgcg ccctataacc tgaaatggag agtgtctgcc gccacagcag
     3841 tatgaaggtt atcgcgaact gaaacgcaac gcgccagtcg gaatgatggt caccagcggt
     3901 gagcaccacg gcacactgca atcttttcgt acgctttcag aaaccggtat cgacattatg
     3961 cagccggatg ttggctggtg cggtggctta accacgctgg tggaaattgc cgcaatcgcc
     4021 aaatccaggg ggcaactggt ggtgccgcac ggttcgtctg tttactccca ccatgcggtg
     4081 atcaccttca ctaatacgcc attcagcgaa ttcctgatga ctagtccgga ttgttcaacg
     4141 atgcgtccgc agtttgaccc gattctgctc aatgagccgg ttccggtgaa tggtcgtatt
     4201 cataaatcag tgcttgataa acccggtttt ggcgtcgaac tcaatcgtga ctgcaatttg
     4261 aaacgcccct acagccacta atcactccgg gcgttgctgt gacaacggta acgcccacat
     4321 attctaagga gctttttatg agcaccgctt tgcttgacgc cgtggtgaag aaaaaccgtg
     4381 tgcgtttaat tccgtttatg ttggcgctgt atgtgctggc gtttctcgac cgttcgaata
     4441 tcggttttgc caaacagacc taccagattg ataccgggtt gagtaatgaa gcttatgcgc
     4501 tgggagcagg cattttcttt gtggtatatg cgtttctggg tgttccggcg aatcttttga
     4561 tgcgcaaact gggggccaga acctggattg gtacgacaac actgctgtgg ggatttcttt
     4621 cggcagccat ggcatgggcc gatactgaag cgaaatttct gatagttcgc actctgcttg
     4681 gtgctgcgga ggccggattt ttccctggta tgatttatct cacctcgcaa tggtttccgc
     4741 agcgtaatcg cgccagcatt atggggctgt tctatatggg cgcaccgctg gcgttaacac
     4801 tgggatcacc gctttccggc gcgctgctgg agatgcatgg atttatgggg ca
//
LOCUS       DAZKIP010000216         4834 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZKIP010000216 DAZKIP010000000
VERSION     DAZKIP010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.218"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..597
                     /locus_tag="ACHOUK_004633"
     CDS             <1..597
                     /locus_tag="ACHOUK_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFP9899925.1"
                     /translation="LIDHDELLSTLSYDSETGIFKWLKTNSVVRVKGSIAGGVSGGYI
                     CISINNVLYKAHRLAWFYVYKKWPPKFIDHVNGNRLDNRISNLRLATEEQNARNIVGN
                     RLNTSGAIGVSWYKPTGRWKSYVGYKNKTISLGYFDSKEDAAFIAALARKKLYGTYAS
                     KALNCEHELLSQFNNDEDKLAEYLKEKYKRTRKRVKKR"
     gene            1061..1393
                     /locus_tag="ACHOUK_004634"
     CDS             1061..1393
                     /locus_tag="ACHOUK_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899926.1"
                     /translation="MDESRKQFEEYVAKKLRLPFEMITEARNGDRYFAFSSMDIRHSL
                     NDWWALWQASRAAIELDIDWPESNDDLWKDGEEGAYAMGYEDGRDKTVIAVMKAIRAA
                     GIKEKNFR"
     gene            1390..1800
                     /locus_tag="ACHOUK_004635"
     CDS             1390..1800
                     /locus_tag="ACHOUK_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFP9899927.1"
                     /translation="MKQTIFLRTKQQQQAAINAILATPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCGKWLKPEQWKVLLISGHAVATKQEADVLPGLEGEYVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
     gene            1797..1988
                     /locus_tag="ACHOUK_004636"
     CDS             1797..1988
                     /locus_tag="ACHOUK_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001254209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFP9899928.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKPKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARNTRGLT"
     gene            2254..2586
                     /locus_tag="ACHOUK_004637"
                     /pseudo
     CDS             2254..2586
                     /locus_tag="ACHOUK_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309226.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
     gene            2857..3123
                     /locus_tag="ACHOUK_004638"
                     /pseudo
     CDS             2857..3123
                     /locus_tag="ACHOUK_004638"
                     /inference="COORDINATES: protein motif:HMM:NF022018.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
     gene            3220..3525
                     /locus_tag="ACHOUK_004639"
                     /pseudo
     CDS             3220..3525
                     /locus_tag="ACHOUK_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309584.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="P22AR C-terminal domain-containing protein"
     gene            3600..4322
                     /locus_tag="ACHOUK_004640"
     CDS             3600..4322
                     /locus_tag="ACHOUK_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor protein"
                     /protein_id="HFP9899929.1"
                     /translation="MNELINSNVIKMTSIEISELTGKRHDNVKRTIETLAKNGVIRHP
                     QIEDCGRINGLGLNQSFRVYVFEGEQGKRDSIIVVAQLSPEFTARLVDRWRELEGATA
                     KIPQTFSEALRLAADLEDQKAELEKQLALAAPKVEFADRVGEASGILIGNFAKVVGIG
                     PNKRFAWMRDHKILIASGARRNVPMQEYMDRGYFTVKETAVNTNHGIQISFTTKITGR
                     GQQWLTRKLLDNGMLKVTGEAA"
     gene            4322..>4834
                     /locus_tag="ACHOUK_004641"
     CDS             4322..>4834
                     /locus_tag="ACHOUK_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFP9899930.1"
                     /translation="MAKPARRKCKICKEWFHPAFSNQWWCSPEHGTKLALERRNKERE
                     KAEKTAEKKRRREEQKQKDKIKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCISC
                     GTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELINRIGQE
                     AVDEIESNHNR"
BASE COUNT         1533 a         1018 c         1156 g         1127 t
ORIGIN      
        1 ctaattgatc acgatgagct tctgtcaaca ttatcatatg actcagaaac aggaatattt
       61 aaatggctaa aaacaaattc agtagtaaga gtaaaaggta gtattgctgg aggtgttagt
      121 ggtggttata tatgcattag tataaataat gttttgtata aggcgcatag acttgcttgg
      181 ttctatgtat acaaaaaatg gcctcctaag tttattgatc atgtaaatgg gaacagactt
      241 gataatagga tttcaaacct aagactggca acagaagagc agaatgcaag aaacattgta
      301 gggaatagat taaacacatc cggtgcaatt ggagtgtctt ggtataagcc aactggcagg
      361 tggaagtctt atgttggtta taaaaataag acaatatcgt tagggtattt cgatagcaaa
      421 gaagatgcag cattcatagc agcactagca agaaagaaac tatatggaac ttatgcgagt
      481 aaagcactta attgcgagca tgagctttta tctcaattta ataatgatga ggataaactt
      541 gcggaatatc ttaaggaaaa atataaaagg actcgaaagc gtgttaaaaa aagataaagg
      601 cctgctggta atcgcaggcc tttttatttg ggggagaggg aagtcatgaa tctggacaag
      661 caagatgcac aaactattag ctcatacata agggcatcaa gaccagatta caaaggtccg
      721 gtattcgtag atttatctcg ccttgaatag atttacatgt ggaaagcaag gttacttacg
      781 cattttgtta ttcgaacgat gactagcaac attacaaaac caatgtaact ggaagaggtg
      841 aatatgagca cactcgcaga ccttattcat gccgatatgg ctgaagatgg agcaaggcgt
      901 aataggtact ggaaatcatc gagccttcca gtttgtgaaa gattcaatca caggccaaaa
      961 ccaaaacgta gccgacgaga caaagtgttg aaaaaactca tgcaaattaa catggctggt
     1021 tttgtcagat tcgtgagtga aacgactaac ggtgattgat atggacgaat caagaaagca
     1081 gtttgaggaa tacgttgcca aaaaattgag attaccattc gagatgataa ccgaggcaag
     1141 aaatggtgat aggtacttcg cattttcaag catggatatt cgtcactcct taaatgattg
     1201 gtgggcttta tggcaggcat cgcgagcagc tattgaactg gatatcgact ggcccgaatc
     1261 gaatgacgac ctttggaaag atggtgaaga aggtgcttat gcgatgggtt atgaggatgg
     1321 gcgtgacaaa acggtaattg cagtaatgaa agctatcaga gccgctggaa ttaaagagaa
     1381 gaattttcga tgaagcaaac aatcttcctc cgaactaagc aacaacagca agctgcaata
     1441 aatgccatcc tcgcaacacc actcgataaa gacaagccag tcaccatccg cattactgac
     1501 tacaagcgca accttgacca gaacgcaaaa tttcacgcga tgctggcgga tatcgctcgt
     1561 caggttcaat ggtgcggcaa atggttaaaa ccagaacaat ggaaggtttt gttgatcagc
     1621 ggtcatgcag tggcaacaaa gcaggaagct gatgttttgc ccgggcttga aggcgaatac
     1681 gtcaacattc gcgaaagcag cgcgcagatg agtgtgaagc gtatggcaag tctgattgag
     1741 tacacgacag catgggctat tggtcagggt gtcagattta ccgacaggag gtacgaatga
     1801 gacgacagcg acgaagtatc accgacataa tctgtgaaaa ctgcaaatac cttccaacga
     1861 aacgctccag aaataaaccc aagccaatcc caaaagaatc tgacgtaaaa accttcaact
     1921 acacggctca cctgtgggat atccggtggc taagacatcg tgcgaggaat acaagggggt
     1981 tgacgtgatg atttatccgg ggctatattc ctcacacgcc agcaaaatct ggcgtcggga
     2041 ttggcgtcct ggatagagac cgcgacagat acacgccgcg agcgtgtttt ttattgtcgt
     2101 atgcacgcgc acatctgaat tatggtgggc tgtgtggggg cggagagatc cgcgccggtc
     2161 ggtttcccgg ttacgccaac cctgcacagt tcaccaccag acgattggcg tcgtcggtgg
     2221 tgagttatta agaaaccact cgagggcgtc attatgacaa ctcaaatctc tgttgaaact
     2281 ctctccccga tcacccataa ccagattcct gttattacca ccgaactttt ggcgcagttg
     2341 tacggtgctg aagttaagaa cattcaaaat aactacgcca gaaatgctga gcgctttatt
     2401 gaaggaaagc attttttcaa agtggctggc gatgccctga aaaatttgcg ggttacttta
     2461 agtaactcac aaaatttgca accatcttta agaggtttgc aaatttcccc caaaactcgc
     2521 tccctcatcc tctggacaga acgaggcgca gcccgtcacg ccaaaatgct cgaaaccgat
     2581 caggcttgac gttataggtt gttacttgta gatttatctt cgtgaggcgt cagaacctct
     2641 ctaaagcgga caaacccaac cccgatagtg ttggattttt tatgcctgtc attttatgga
     2701 cgcattgcgc ggtcataccc cgaacaatgt cgggagggcg acgaatacaa cacccgaaag
     2761 gggaatgagt ccgcggttct ttaggccgtt ctgaacctcc cgacaccaca atcagaagtg
     2821 gtaattcaga aaaagctaaa ggaggtcgtt atgaccatgc aactcgccgt agaatccctc
     2881 gtcgttgtta cttactcgaa cattcccgta ataacaacgg agttattggc aacactatac
     2941 aaaacagaac aaaagcatat acggcaaaat ttcaaacgta acgaatgtcg ttttattgca
     3001 ggaaaacact tctttaaagt aagtggtagt gaactcgatg atttgagaac ctctcaaaga
     3061 ggtttgcaaa tttcccccaa aactcgctcc ctcatcctct ggacagaacg aggcgcagcc
     3121 cgtcacgcca aaatgctaga aaccgatcag gcgtgggaag tgttcgaaaa actggaagac
     3181 tgctatttca gccagacttc gaaaagcgca ggtcatcacg agaagaaaac cagcgagctt
     3241 tctgcaaaag atgcaaacag tctggtatgg ttgtgggatt atgccaaccg ctcacaggca
     3301 ttgttccgcg aactgtatcc ggcgctaaaa caaattcaat cgaactattc cggcagatgc
     3361 tacgactacg gccatgagtt ttcgtatgtt atcggaatgg cgagagatgt tttaatcaat
     3421 cacacacgag atgttgatat taatgatcca gacggaccaa cgaatctttc cgcatgggta
     3481 agacttaaga acaaagaatt acctcctgca ctgcatcgct actgacaatt gacaacttaa
     3541 caaacccagc ttcggctggg ttttttattg gtgaattttc aatatgagag gacatgacaa
     3601 tgaacgagct gataaatagc aacgtcatca aaatgacgag cattgaaatc tctgagctta
     3661 caggtaagcg tcatgacaat gtgaaacgta ctatcgaaac gctggctaaa aatggtgtta
     3721 tccggcatcc tcaaattgag gattgtggaa gaatcaatgg gttaggctta aatcaaagtt
     3781 ttcgtgtgta tgtattcgaa ggcgaacaag gtaagcgcga tagcatcatt gttgtcgctc
     3841 agttgtcgcc ggaattcacc gctcgccttg ttgaccgctg gcgagaactc gaaggggcaa
     3901 ccgcgaaaat accacaaacc ttttctgagg cattgcgcct tgcggccgac cttgaagacc
     3961 agaaggctga actggagaaa cagcttgctc tcgcagcacc taaagttgag tttgccgatc
     4021 gagttggcga ggccagcgga attttgattg gaaactttgc aaaggttgtt ggtattggtc
     4081 caaacaaacg gtttgcgtgg atgcgcgatc acaaaatcct tattgcttca ggtgcccggc
     4141 gcaatgtgcc aatgcaggaa tatatggatc gcggctattt cacagtgaaa gaaacagcgg
     4201 tcaatacaaa tcacggaata cagatatcgt tcaccacaaa aatcaccggg cgtggtcaac
     4261 agtggctgac aagaaagctg ctagataacg gaatgcttaa agtaacaggg gaggctgctt
     4321 aatggctaaa ccagcgcgaa ggaaatgcaa aatctgtaag gaatggtttc acccggcatt
     4381 ctcaaatcag tggtggtgca gcccggaaca cggaactaaa ttagcactcg aacgacgaaa
     4441 taaagaacgc gaaaaggcgg aaaaaacagc agagaagaaa cgacgacgag aggagcagaa
     4501 acagaaagat aaaattaaga ttcgaaaact cgccttaaag ccccgcagtt actggattaa
     4561 acaagcccaa caagccgtaa acgccttcat cagagaaaga gaccgcgact taccatgtat
     4621 ctcgtgcgga acgctcacgt ctgctcagtg ggatgccgga cattaccgga caactgctgc
     4681 ggcacctcaa ctccgatttg atgaacgcaa tattcacaag caatgcgtgg tgtgcaacca
     4741 gcacaaaagc ggaaatctcg ttccgtatcg cgtcgaactg attaatcgca tcgggcagga
     4801 agcagtagac gaaatcgaat caaaccataa ccgc
//
LOCUS       DAZKIP010000217         4828 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZKIP010000217 DAZKIP010000000
VERSION     DAZKIP010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4828
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.219"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            22..1356
                     /gene="garP"
                     /locus_tag="ACHOUK_004642"
     CDS             22..1356
                     /gene="garP"
                     /locus_tag="ACHOUK_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFP9899931.1"
                     /translation="MILDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            1372..2142
                     /gene="garL"
                     /locus_tag="ACHOUK_004643"
     CDS             1372..2142
                     /gene="garL"
                     /locus_tag="ACHOUK_004643"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFP9899932.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            2172..3062
                     /gene="garR"
                     /locus_tag="ACHOUK_004644"
     CDS             2172..3062
                     /gene="garR"
                     /locus_tag="ACHOUK_004644"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFP9899933.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            3159..4304
                     /gene="garK"
                     /locus_tag="ACHOUK_004645"
     CDS             3159..4304
                     /gene="garK"
                     /locus_tag="ACHOUK_004645"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFP9899934.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            4337..4713
                     /gene="rnpB"
                     /locus_tag="ACHOUK_004646"
     ncRNA           4337..4713
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHOUK_004646"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         1125 a         1155 c         1391 g         1157 t
ORIGIN      
        1 taagtacccg aggtaaataa aatgattctg gacaccgttg acgaaaaaaa gaaaggcgtg
       61 catacccgct atttaatatt actgattatt tttattgtta ccgccgttaa ctacgccgat
      121 cgtgcaacgc tgtctattgc tggtaccgaa gtggcaaaag agttgcagtt aagtgcggtt
      181 tcgatgggtt acatcttttc tgcttttggc tgggcctact tgctgatgca aatccccggc
      241 ggctggctgc ttgataagtt tggctcgaaa aaagtttaca cctacagcct ctttttctgg
      301 tcgctattca ccttcctgca aggctttgtt gatatgttcc cgctggcctg ggcagggatc
      361 tccatgttct ttatgcgctt tatgctcggc ttctcggaag cgccatcatt cccggcgaac
      421 gcccgtattg tcgccgcctg gttcccgacg aaagaacgtg gtactgcctc cgccatcttt
      481 aactcggcgc aatatttctc gctggcgctc ttttcgcctc tgcttggctg gctgactttc
      541 gcctggggct gggagcacgt ctttaccgtt atgggggtga ttggttttgt gctgacggcg
      601 ctgtggatca aattgattca taacccgaca gatcacccac gtatgtctgc ggaagagctg
      661 aagtttatct ctgaaaatgg cgcggtggtc gatatggacc acaaaaagcc gggcagtgcg
      721 gcagcaagcg gacccaaact gcattacatc aagcaattgc tctctaaccg catgatgttg
      781 ggcgtatttt tcggacaata ttttatcaac accatcacct ggttcttcct cacctggttc
      841 ccgatttatc tggtgcagga aaaaggtatg tcgattctga aagtgggtct ggtcgcctcg
      901 attccagcac tgtgtggttt tgcgggcggc gtgctgggag gtgtcttctc ggattatctg
      961 atcaaacgcg gtttatccct gaccctggca cgtaagctac cgattgtgct gggaatgttg
     1021 ctggcttcca ccatcatctt atgtaactac accaacaaca ccacgctggt ggtcatgctg
     1081 atggcgctgg ctttctttgg taaaggattt ggtgcgctgg gctggccggt gatttctgac
     1141 accgcgccta aagagattgt tggcctctgc ggcggcgtct ttaacgtctt tggcaatgtt
     1201 gcctccattg tcactccact ggtgattggc tacctggtaa gtgaactgca ctccttcaat
     1261 gcagcactgg ttttcgtggg atgttcagcg ctaatggcga tggtctgcta cctcttcgta
     1321 gttggcgaca ttaaacgtat ggaattgcag aaataagcaa aggtataagc gatgaataac
     1381 gatgttttcc cgaataaatt caaagccgca ctggctgcga aacaggtaca aattggttgc
     1441 tggtcagcac tctctaaccc gattagcact gaagttcttg gtttggctgg gtttgactgg
     1501 ctggtgctgg atggcgaaca tgcgccaaac gatatttcaa cgtttattcc gcagttaatg
     1561 gccctgaaag gcagcgccag cgcgccagta gtgcgagtgc cgaccaacga gccggtaatt
     1621 attaagcgtc ttctggatat cggtttctat aacttcctga ttccttttgt agaaacaaaa
     1681 gaggaagcag agcaggcggt ggcatcgacg cgttatccgc cagaaggcat tcgcggcgtc
     1741 tccgtttctc accgcgccaa tatgtttggc accgtggcgg attatttcgc tcagtcgaac
     1801 aagaacatca ctattctggt ccagatagaa agtcagcagg gcgtagataa cgtcgatgct
     1861 attgccgcta ccgaaggcgt agacggcatc ttcgtcggcc ccagcgatct ggccgcggca
     1921 ttaggccatc tcggcaatgc atcacacccg gatgtacaaa aagcaattca gcacattttt
     1981 aaccgtgcca gcgcgcacgg caaacccagc ggtatcctcg cgccggtcga agccgatgcg
     2041 cgtcgttatc tggaatgggg cgcgacgttt gtggctgtcg gcagcgatct cggcgtcttc
     2101 cgctctgcca ctcagaaact ggctgatacc tttaaaaaat aaccaccacc gcaacaagag
     2161 agatgattga tatgactatg aaagttggtt ttattggcct ggggattatg ggtaaaccaa
     2221 tgagtaaaaa ccttctgaaa gcaggttact cgctggtggt tgctgaccgt aacccagaag
     2281 ctattgctga cgtgattgct gcaggtgcag aaacagcttc tacggctaaa gcgatcgctg
     2341 aacagtgcga cgtcatcata accatgctgc caaactcccc tcatgtgaaa gaggtggcgc
     2401 tgggtgagaa tggcattatt gaaggcgcga agccaggtac ggtattgatc gatatgagtt
     2461 ctatcgcacc gctggcaagc cgtgaaatca gcgaagcgct gaaagcgaaa ggcattgata
     2521 tgctggatgc tccggtgagc ggcggtgaac cgaaagccat cgacggtacg ctgtcagtga
     2581 tggtaggcgg cgacaaggct attttcgaca aatactatga tttgatgaaa gcgatggcgg
     2641 gttccgtggt gcataccggg gaaatcggtg caggtaacgt caccaaactg gcaaatcagg
     2701 tcattgtggc gctgaatatt gccgcgatgt cagaagcgtt aacgctggca actaaagcgg
     2761 gcgttaaccc ggacctggtt tatcaggcaa ttcgcggtgg actggcgggc agtaccgtgc
     2821 tggatgccaa agcgccgatg gtgatggacc gcaacttcaa gccgggcttc cgtattgatc
     2881 tgcatattaa ggatctggcg aatgcgctgg atacttctca cggcgtcggc gcacaactgc
     2941 cgctcacagc tgcggttatg gagatgatgc aggcactgcg tgcagatggt ttaggaacgg
     3001 cggatcatag cgccctggcg tgctactacg aaaaactggc gaaagtcgaa gttactcgtt
     3061 aatgacgtcg tgcccggatg gcgtattgca atccgggcct ggaatccagg ccgaataaga
     3121 gaaacgccct ccggcgtcat gtggtaacag gcataggtat gaaaatcgta atcgccccag
     3181 actcttataa agaaagttta tctgccagcg aggttgcgca ggcgatagaa aaaggatttc
     3241 gggaaatttt tcctgatgca cagtacgttt ctgttcctgt tgccgacggt ggcgaaggaa
     3301 cggtggaagc gatgattgca gccacccagg gttccgaacg tcacgcctgg gttacagggc
     3361 cgctgggcga gaaagtgaat gccagttggg ggatctccgg cgatggcaaa accgcgttta
     3421 ttgaaatggc ggcggccagt gggctggagc tggtacctgc ggaaaaacgc gatccactcg
     3481 tgaccacttc acgcggcaca ggcgagttaa tcctgcaggc gctggagagc ggcgcgacaa
     3541 acattattat cggcattggc ggcagcgcta caaatgatgg cggcgcaggc atggtacagg
     3601 cgctgggggc gaaattatgc gacgccaacg gcaatgaaat tggttttggc ggcggtagtc
     3661 ttaatactct gaatgatatt gatatttccg gcctcgatcc gcgcttaaaa gattgcgtca
     3721 ttcgcgtcgc ttgtgatgtc accaatccgc tggtgggcga taacggcgca tcgcgcatct
     3781 ttggcccaca aaagggagcc agtgaagcga tgattgttga gctggacaat aacctctctc
     3841 actatgccga ggtcattaaa aaagcgctgc atgttgatgt gaaagatgtc cccggcgcag
     3901 gagctgcggg tggtatgggc gcggcgctaa tggcgtttct tggtgcggaa ctgaaaagtg
     3961 gtattgaaat cgtcactacg gcgctgaatc tggaggaaca tattcacgat tgtacgctgg
     4021 tgatcaccgg tgaagggcgt attgacagcc agagtattca cggcaaggta ccgattggtg
     4081 tcgcaaacgt ggcgaagaag taccataaac cggtgattgg cattgcgggt agcctgaccg
     4141 atgatgttgg cgttgtacat cagcatggca ttgatgcggt cttcagcgta ttgaccagca
     4201 taggtacgtt ggacgaagca ttccgcgggg cttatgacaa tatctgccgt gcttcacgta
     4261 atatcgccgc gacactggcg attggaatgc gcaacgcggg gtgacaaggg cgcgcaaacc
     4321 ctctatactg cgcgccgaag ctgaccagac agtcgccgct tcgtcgtcgt cctcttcggg
     4381 ggagacgggc ggaggggagg aaagtccggg ctccataggg cagggtgcca ggtaacgcct
     4441 gggggggaaa cccacgacca gtgcaacaga gagcaaaccg ccgatggccc gcgcaagcgg
     4501 gatcaggtaa gggtgaaagg gtgcggtaag agcgcaccgc gcggctggta acagtccgtg
     4561 gcacggtaaa ctccacccgg agcaaggcca aataggggtt cataaggtac ggcccgtact
     4621 gaacccgggt aggctgcttg agccagtgag cgattgctgg cctagatgaa tgactgtcca
     4681 cgacagaacc cggcttatcg gtcagtttca cctgatttac gtaaaaaccc gcttcggcgg
     4741 gtttttgctt ttggtgcaca gggtaagatg aataaatatc tacggtatta ataatagacc
     4801 ttgtaagaag ttattaagtc tatattgc
//
LOCUS       DAZKIP010000218         4785 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZKIP010000218 DAZKIP010000000
VERSION     DAZKIP010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.220"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            164..730
                     /gene="slp"
                     /locus_tag="ACHOUK_004647"
     CDS             164..730
                     /gene="slp"
                     /locus_tag="ACHOUK_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFP9899935.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            886..1416
                     /gene="dctR"
                     /locus_tag="ACHOUK_004648"
     CDS             886..1416
                     /gene="dctR"
                     /locus_tag="ACHOUK_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFP9899936.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(1477..2505)
                     /gene="chuS"
                     /locus_tag="ACHOUK_004649"
     CDS             complement(1477..2505)
                     /gene="chuS"
                     /locus_tag="ACHOUK_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hematinate-forming heme oxygenase ChuS"
                     /protein_id="HFP9899937.1"
                     /translation="MNHYTRWLELKEQNPGKYARDIAGLMNIREAELAFARVTHDAWR
                     MHGDIREILAALESVGETKCICRNEYAVHEQVGTFTNQHLNGHAGLILNPRALDLRLF
                     LNQWASVFHIKENTARGERQSIQFFDHQGDALLKVYATDNTDMAAWSELLARFITDEN
                     TPLELKAVDAPVVQTRADATVVEQEWRAMTDVHQFFTLLKRHNLTRQQAFNLVADDLA
                     CKVSNSALAQILESAQQDGNEIMVFVGNRGCVQIFTGVVEKVVPMKGWLNIFNPTFTL
                     HLLEESIAEAWVTRKPTSDGYVTSLELFAHDGTQIAQLYGQRTEGEQEQAQWRKQIAS
                     LIPEGVAA"
     gene            complement(2554..4536)
                     /gene="chuA"
                     /locus_tag="ACHOUK_004650"
                     /gene_synonym="shuA"
     CDS             complement(2554..4536)
                     /gene="chuA"
                     /locus_tag="ACHOUK_004650"
                     /gene_synonym="shuA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent heme/hemoglobin receptor
                     ChuA/ShuA"
                     /protein_id="HFP9899938.1"
                     /translation="MSRPQFTSLRLSLLALAVSATLPTFAFATETMTVTATGNARSSF
                     EAPMMVSVIDTSAPENQTATSATDLLRHVPGITLDGTGRTNGQDVNMRGYDHRGVLVL
                     VDGVRQGTDTGHLNGTFLDPALIKRVEIVRGPSALLYGSGALGGVISYDTVDAKDLLQ
                     EGQSSGFRVFGTGGTGDHSLGLGASAFGRTENLDGIVAWSSRDRGDLRQSNGETAPND
                     ESINNMLAKGTWQIDSAQSLSGLVRYYNNDAREPKNPQTVEASESSNPMVDRSTIQRD
                     AQLSYKLAPQGNDWLNADAKIYWSEVRINAQNTGSSGEYREQITKGARLENRSTLFAD
                     SFASHLLTYGGEYYRQEQHPGGATTGFPQAKIDFSSGWLQDEITLRDLPITLLGGTRY
                     DSYRGSSDGYKDVDADKWSSRAGMTINPTNWLMLFGSYAQAFRAPTMGEMYNDSKHFS
                     IGRFYTNYWVPNPNLRPETNETQEYGFGLRFDDLMLSNDALEFKASYFDTKAKDYIST
                     TVDFAAATTMSYNVPNAKIWGWDVMTKYTTDLFSLDVAYNRTRGKDTDTGEYISSINP
                     DTVTSTLNIPIAHSGFSVGWVGTFADRSTHISSSYSKQPGYGVNDFYVSYQGQQALKG
                     MTTTLVLGNAFDKEYWSPQGIPQDGRNGKIFVSYQW"
BASE COUNT         1278 a         1169 c         1082 g         1256 t
ORIGIN      
        1 ggatactcat gccacgcccc aaaataacgg aattgaacta aacagaaggg atataaacat
       61 cagacaggtt tacgttacta tcaggcatat cgcctcagaa tcagatgaaa actataaaga
      121 aatatctatt atggttttaa tatttgttga taaggatagt aacatgaaca tgacaaaagg
      181 tgcactcata ctcagccttt catttttgct tgcagcatgt agttcaattc cgcaaaatat
      241 caaaggaaat aaccaacctg atattcaaaa aagttttgtt gctgttcata accagccggg
      301 gttatatgtt ggtcaacaag cgcgctttgg tgggaaggtt atcaacgtta tcaatggcaa
      361 aacggatacg ttgttagaaa tcgctgtatt accgttggat agctatgcga agcctgatat
      421 tgaagccaac tatcagggcc gactgctcgc cagacaaagc ggcttccttg atccagtgaa
      481 ctatcgtaat cactttgtta ccatcctcgg caccattcag ggtgaacaac ctggctttat
      541 caataaagtc ccgtataact tcctggaagt gaatatgcag ggcatccagg tgtggcattt
      601 gagagaagtg gttaatacca cctataacct gtgggattac ggctatggtg cattctggcc
      661 ggaaccgggc tggggtgcgc cttactacac caatgcggtg agtcaggtaa cacctgagct
      721 ggtcaaataa caccaccgaa agatgcagat gctttctcag catctgcatc atgcattaca
      781 tcaaattaat acacagtaag ctaactatta ttattataag ccctgtcctg ttaattacct
      841 ttggcaaact gattataaag ttaatgtccg caccaggagt cggttatgtt tcttataatt
      901 accagggata cgatgttctt caccgcgatg aaaaacattc tgagtaaagg taatgtcgtt
      961 catatacaga acgaagaaga gatcgacgta atgttgcatc agaatgcctt cgtcattatt
     1021 gatacattaa tgaataatgt atttcattct aattttctca ctcaaattga acgattaaaa
     1081 cctgtccatg tcattatttt ctcccccttt aatattaaac gctgcctggg gaaagtgccg
     1141 gtgacctttg ttccgcggac tatcactatc attgattttg tcgcactcat caatggcagt
     1201 tactgctctg tgcctgaagc gaatgtgtca ctttcgcgca agcaacatca ggttctgagc
     1261 tgcattgcga atcaaatgac aacggaagat attctggaga aactgaaaat atcgctaaaa
     1321 acgttctact gccataaaca caatatcatg atgatcctca atcttaagcg gatcaatgag
     1381 ctggtacgcc atcagcatat tgattatctg gtgtgaacga ttgatcaata taaagaggcc
     1441 cagcaataaa tagccagacc tcccgttaat tatacgttat gcagcaacgc cttccggtat
     1501 cagcgaagca atttgcttac gccattgcgc ttgctcctgt tcgccttctg tacgttgacc
     1561 ataaagttgc gctatctgcg taccatcatg ggcaaacaat tccagactgg ttacgtagcc
     1621 atcgctggtc ggtttacggg taacccaggc ttcagcaatg ctctcttcta atagatgaag
     1681 agtaaacgtc gggttgaaaa tattcagcca acctttcatt ggcaccactt tttctaccac
     1741 accggtgaaa atctgtacgc agccacggtt gccaacaaac accatgattt cattaccatc
     1801 ctgctgtgca gattcaagaa tttgcgccaa cgcactgttg gatactttgc aggccaaatc
     1861 gtctgccacc agattgaacg cctgttggcg cgtcaggttg tggcgcttga gcaacgtaaa
     1921 aaactgatga acgtcggtca tcgcccgcca ctcttgctcg accacagtgg catcggctcg
     1981 cgtttgaaca actggcgcat caacggcttt taactcaagc ggcgtattct catcggtgat
     2041 aaaccgtgcc agaagctcac tccatgccgc catatcggta ttgtcggtgg cataaacttt
     2101 tagtaatgca tcgccctgat gatcaaagaa ctgaatactc tggcgttcgc cacgagccgt
     2161 gttttctttg atgtggaaaa cactggccca ttgattgaga aacagacgta aatccagcgc
     2221 gcgcggattg aggatcaatc cggcatgtcc gttcaaatgc tggtttgtga acgtaccaac
     2281 ttgctcatgg actgcatatt cattacgaca aatacatttg gtttcgccaa cactttcgag
     2341 cgccgccaga atttcacgga tatcgccgtg catccgccac gcatcgtgcg tgacgcgtgc
     2401 aaatgccagt tctgcttctc taatattcat taaccctgcg atgtcacgcg cgtactttcc
     2461 gggattttgt tcttttaact caagccagcg tgtgtagtgg ttcattgtct ctcttccttc
     2521 cagataaatg ccccgaaata tcggggcaga tgattaccat tgataactca cgaaaatttt
     2581 tccgttacga ccatcctgtg ggatgccttg cggcgaccag tactctttgt cgaaagcgtt
     2641 acccaacacc aaagtagtgg tcataccttt gagcgcctgt tgtccttgat aactgacgta
     2701 gaaatcattc acgccatagc ctggttgttt gctgtaactg ctgctgatat gtgttgagcg
     2761 atcggcaaac gtaccaaccc acccaacaga gaagccactg tgagcgatcg gaatattcag
     2821 agtgctggta acagtatccg ggttaatgct ggagatgtat tcgccggtat cggtgtcttt
     2881 gccgcgggta cggttatagg ccacatcaag gctaaacaga tcagtggtat atttcgtcat
     2941 cacatcccag ccccagattt tggcgttcgg gacgttatac gacatagtcg tcgccgccgc
     3001 gaaatcgacg gtcgtggaga tgtaatcctt cgctttggta tcaaagtagc tggctttaaa
     3061 ttccagagca tcattggaca acatcaggtc atcaaaacgc agcccaaaac cgtactcctg
     3121 agtttcgtta gtttccggac gtaagttcgg gtttggcacc caatagttgg tatagaagcg
     3181 accaatcgag aagtgcttag aatcgttata catttcgccc atcgtcgggg cgcggaatgc
     3241 ctgggcatat gagccaaata acatcagcca gttagtcgga ttgatagtca tccccgcacg
     3301 agatgaccat ttgtcggcat caacatcttt gtaaccgtca ctgctaccgc gataactgtc
     3361 atagcgggtt ccgccaagca gggtaatcgg cagatcgcgt aaggtgatct catcctgtag
     3421 ccagccggag ctaaaatcga tttttgcttg cgggaagccc gtcgtcgcgc cgcccggatg
     3481 ttgttcctga cgataatact caccgccata tgtcagtaag tgagaagcga aactgtcggc
     3541 aaagagagtg gaacggttct ccagcctggc tccttttgtt atctgttcac gatactcgcc
     3601 ggaactcccc gtgttttgcg cattaatacg gacttccgac caataaattt ttgcatctgc
     3661 atttaaccag tcgttgccct gcggggcgag tttataagaa agctgcgcat cgcgttgaat
     3721 tgttgaacga tcaaccatcg ggttgctgct ttcagaagcc tcaacggtct gcggattttt
     3781 tggttcacgc gcgtcgttgt tgtagtaacg cactaaaccg ctcagagact gggctgaatc
     3841 aatttgccag gtccctttcg ccagcatgtt attaatggac tcgtcattcg gcgcggtttc
     3901 accattgctc tggcgtaaat caccccgatc gcgactggac caggccacaa taccatccag
     3961 attttcagtt cgcccaaacg cgctcgcgcc taatcccagg ctatggtccc ccgtgccgcc
     4021 agtaccaaag acacgaaaac cactgctttg tccttcctgc aataaatctt ttgcatcgac
     4081 cgtatcgtag gagatcactc cacccagcgc gccactgcca tacagtaatg ctgaaggtcc
     4141 acgaacaatc tcaacacgct tgatcagcgc cggatcgaga aaagtgccat tcaggtgtcc
     4201 ggtatccgtt ccctgacgaa caccatcgac aagaaccagc acgccgcgat gatcatagcc
     4261 acgcatattt acatcctgac cgttggttcg tccggtacca tccagagtaa ttccaggaac
     4321 atgacgcagc agatcggtgg ctgaagtagc cgtttgattt tcaggagcgg aagtgtcgat
     4381 gacgctgacc atcataggcg cttcgaagga actacgggca ttccccgttg ccgtaacggt
     4441 catggtttca gtagcaaaag caaacgttgg caaggtggca gaaacagcta aggccaataa
     4501 actcaaacgc aacgaggtaa attgcggacg tgacatagcg attctccatg aggaaaataa
     4561 aaaatgctgg ctgcctgggt taataccggg gtggctggcg aaaaataaat ttatttaaaa
     4621 ttcagagcga taatattcgc cgggtgagtt tacaagcgtt tatatcccat gaaattgcct
     4681 gataactcgg caattacgca aaagcacgcc ctctttgtca ggcaaaacga tatgccccat
     4741 caggagtgaa gagagctgtg cgttatccct tgcaaatgct atggt
//
LOCUS       DAZKIP010000219         4736 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZKIP010000219 DAZKIP010000000
VERSION     DAZKIP010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.221"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(84..776)
                     /locus_tag="ACHOUK_004651"
     CDS             complement(84..776)
                     /locus_tag="ACHOUK_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001337214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFP9899939.1"
                     /translation="MKKETLADRLNLAMEQSGMSQGALAKASGVAQPTIWRLTSGNAR
                     GSTKIVEIANALGVRTEWLSSGIGPMRNDGQQSGKPAVNHSKYFKIDVLDIEVSAGPG
                     VINREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITV
                     KSFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKV
                     IGSMPQTYRKHG"
     gene            887..1109
                     /locus_tag="ACHOUK_004652"
                     /pseudo
     CDS             887..1109
                     /locus_tag="ACHOUK_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201115.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
     gene            1140..1679
                     /locus_tag="ACHOUK_004653"
     CDS             1140..1679
                     /locus_tag="ACHOUK_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HFP9899940.1"
                     /translation="MQPLTYQQTSGFSPTAVINRSQIKQVPGHEKIRDAVRAWSAVDN
                     QDVVATLIVNEYREQGGGTIDFPDDVSRTRQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGYLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEISEGIVSMFRV
                     DPELAGPLMAMVTTMLGAI"
     gene            1676..2695
                     /locus_tag="ACHOUK_004654"
     CDS             1676..2695
                     /locus_tag="ACHOUK_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFP9899941.1"
                     /translation="MTGSEMAKAGLLEQNRLSGANRNTLIAGGIMANTAEIFNFPVPD
                     AAQKEPRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRGIFIQSGRNIGINNVVSEWSTLPESGKKNKVYLK
                     EVNLPESGKKSLPKSGKGTYPNQVNTKDKLTKDNIKPYSSENSGESSDLPENDLPVVK
                     ADAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHR
                     DMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTASKPKLDLTNTDWI
                     YGVDL"
     gene            2692..3393
                     /locus_tag="ACHOUK_004655"
     CDS             2692..3393
                     /locus_tag="ACHOUK_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFP9899942.1"
                     /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNEVNEIRRQWVLAFRENGITSMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVIAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELTCMTARINRGEAIPEPVKQLPVMGGRPLNRVQALAK
                     IAEIKAKFGLKGASV"
     gene            3390..3674
                     /locus_tag="ACHOUK_004656"
     CDS             3390..3674
                     /locus_tag="ACHOUK_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFP9899943.1"
                     /translation="MTGKEAIIHYLGTHNSFCAPDVAALTGATVTSINQAAAKMARAG
                     LLVIEGKVWRTVYYRFATKEEREGKVSTNMIFKECRQSAAMKRVLLVYRT"
     gene            3851..4099
                     /locus_tag="ACHOUK_004657"
     CDS             3851..4099
                     /locus_tag="ACHOUK_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899944.1"
                     /translation="MHELFVLVLSTCASLSNMSGCSVEVVNVNTTKEPVNVFYSRKDC
                     EESMKNIMLNHALYHEVSGREPYMAKCEQVFVSKSLMK"
     gene            4143..4424
                     /locus_tag="ACHOUK_004658"
     CDS             4143..4424
                     /locus_tag="ACHOUK_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899945.1"
                     /translation="MEDYLVFGLGYEGDIKNDEAGLDKISVVTKSVVRSTNSNEPVVY
                     QVTEFKEFNVVRHQAFDSEYYNIAFDVLPSRVRIDAAIRKYHPKKSSSA"
     gene            4515..4616
                     /locus_tag="ACHOUK_004659"
     CDS             4515..4616
                     /locus_tag="ACHOUK_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899946.1"
                     /translation="MRTYNPNSLLPSLMQKCTCGSLHPTFDLCGGEA"
     gene            4613..>4736
                     /locus_tag="ACHOUK_004660"
     CDS             4613..>4736
                     /locus_tag="ACHOUK_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899947.1"
                     /translation="MNLPQDGIKLHRGNFTAIGQQIQPYLENGKCFRMVLKPWRE"
BASE COUNT         1344 a         1078 c         1159 g         1155 t
ORIGIN      
        1 atccattcta taaattatga ggatgtcagc atgtctcacc aaaacatcat ctggttatct
       61 tgatgaaatc tgtcattaat aatctaccca tgttttctat atgtctgtgg catgctccca
      121 ataactttcc caaatataaa tacccgattc atttcgtctt tttcgatcgg gtcccacggt
      181 gagtagctct tgttatcaga gataaccagc agcttatcct tcatcatttg caggcgcttt
      241 acatgggctg tgtcgtcgta cagaaacgca tagataccat caccgtcgaa agatttaaca
      301 gtgatatcaa cgaacagaag atcacctggt tcgatcgttc ctgacatgct gtcaccgcgt
      361 acgttaatga tgcggatatt ttctgccttc ctgccatcga acatgtgacg agcatcgtca
      421 aacgagtact caaccgagcg taggacttct acaaactcac ggttgatgac acccggccca
      481 gcactgactt ctatatcaag aacgtcaatc ttgaagtatt tggaatggtt gacagcaggc
      541 ttccctgatt gttgaccgtc atttctcatc gggcctatgc ctgatgagag ccactctgtt
      601 cgaacaccca atgcattagc tatttcaaca atttttgttg agccgcgtgc gttgccgctt
      661 gtcagtctcc agattgtggg ttgagctacg ccagacgcct ttgcaagagc gccttgagac
      721 attccagatt gttccatcgc taggtttaag cgatcagcaa gagtttcttt tttcataagt
      781 tttaatttat acgcttgcgt attgatggtc aaaacacgtt ttgctattga ttggattaat
      841 acgcattgct attatccatt cattgtaata ccaataggaa ttgataatga caaatcaaac
      901 cattcaactc gcaatcagta ttacaggtag tcaaaaacga ctggcagatc tatgcggtgt
      961 agcccactgt ttggcgttgg ctacacggtg gcggaattga tgcccgctat gtaatgaaaa
     1021 ttgtctcagc cactggtgga aagattaaac cagcagatat tcgtcccgac cttgcaccat
     1081 tgtttaacgc gagtaattct gccgcctaaa ctgcggcgtt aactgataag gcaatgatta
     1141 tgcaaccact tacataccaa cagactagcg gatttagccc gactgcggtg ataaatcgtt
     1201 ctcaaataaa acaggtgcca ggccacgaaa aaatccgtga tgccgtccgc gcctggtcgg
     1261 ctgtagataa tcaggatgtc gttgccacac tcattgtgaa tgagtatcgg gagcagggcg
     1321 gcggcaccat cgatttccct gatgatgtca gccgtacacg ccagaagctg ttccgcttcc
     1381 tcgataacaa attcgattct gaaaaatacc gaaataacgt gcgtgaactg accccggcaa
     1441 ttctggcggt actaccgctg gaatatcgcg gttacctggt tgagcaggat agcttcatgg
     1501 ctaggttggc tgaaatggaa aaggaactca gtgaggcaaa acaggctgtc attctcaacg
     1561 caccacgcca ccagaaactg aaggaaatta gtgaaggtat tgtgtcgatg tttcgtgtgg
     1621 acccagagct ggctggtcca ttgatggcga tggttactac catgctgggg gcgatatgac
     1681 aggttcagaa atggcgaaag ccggtctgct ggaacagaac cgactttcag gtgcaaatcg
     1741 taacacactc attgcgggag gaattatggc aaacactgct gagatattca attttccagt
     1801 gccggatgcg gcacaaaagg agccgcgcgt ggcagatctc gatgatggtt atacgcgcat
     1861 tgcaaatgag ttgctggaag ctgtaatgct ggccggatta acacagcacc agcttctggt
     1921 cttcctggct gtcatgcgca aaacatatgg ctttaataaa aaactggact gggtgagcaa
     1981 cgagcaactt tccgagttga ccgggatatt gccgcacaag tgttctgctg caaaaagtgt
     2041 tctggtaaag cgtgggattt ttattcagag cgggcggaat atcggcatta ataatgtggt
     2101 cagtgaatgg tcaacattac ccgaatcagg taagaaaaat aaagtttacc tgaaagaggt
     2161 aaatttacct gaatcaggta agaaaagttt acccaaatca ggtaaaggca cttacccgaa
     2221 tcaggtaaac acaaaagaca aactaacaaa agacaatata aaaccttatt cgtccgagaa
     2281 ttctggcgaa tcctctgacc tgccagaaaa cgaccttcct gtggtgaaag cggatgctgc
     2341 gattcagagc ggcagcaagt gggggacagc agaagacctg accgccgcag agtggatgtt
     2401 tgacatggtg aagaccatcg cgccatcagc cagaaaaccg aattttgcag ggtgggctaa
     2461 cgatatccgc ctgatgcgtg aacgtgacgg acgtaaccac cgcgacatgt gcgtgctgtt
     2521 ccgctgggca tgccaggaca acttctggtc cggtaacgtg ctgagcccgg ccaaactccg
     2581 cgataagtgg acccaactcg aaatcaaccg taacaagcaa caggcaggcg tgacagccag
     2641 caaaccaaaa ctcgacctga caaacacaga ctggatttac ggggtggatc tatgaaaaac
     2701 atcgccgcac agatggttaa ctttgaccgt gagcagatgc gtcggatcgc caacaacatg
     2761 ccggaacagt acgacgaaaa gccgcaggta cagcaggtag cgcagatcat caacggtgtg
     2821 ttcagccagt tactggcaac tttcccggcg agcctggcta accgtgacca gaacgaagtg
     2881 aacgaaatcc gccgccagtg ggttctggct ttccgggaaa acgggatcac ctcgatggaa
     2941 caggttaacg caggaatgcg cgtagcccgt cggcagaatc gaccatttct tccatcaccc
     3001 gggcagtttg ttgcatggtg ccgggaagaa gcatccgtta tcgccggact gccaaacgtc
     3061 agcgagctgg ttgatatggt ttacgagtat tgccggaagc gaggcctgta tccggatgca
     3121 gagtcttatc cgtggaaatc gaacgcgcac tactggctgg ttaccaacct gtaccagaac
     3181 atgcgggcca atgcgctgac tgacgcggaa ttacgacgca aggctgccga tgaactgacc
     3241 tgtatgacag cgcgaattaa ccgtggtgag gcgatacctg aaccagtaaa acaacttcct
     3301 gtcatgggcg gcagacctct aaatcgtgtt caggcgctgg cgaagatcgc agaaattaaa
     3361 gctaagttcg gactgaaagg agcaagtgta tgacgggcaa agaggcaatt attcattacc
     3421 tggggacgca taatagcttc tgtgcgccgg acgttgccgc gctaacaggc gcaacagtaa
     3481 ccagcataaa tcaggccgca gctaaaatgg cacgggcagg tcttctggtt atcgaaggta
     3541 aggtctggcg aacggtgtat taccggtttg ctaccaagga agaacgggaa ggaaaggtga
     3601 gcacgaatat gatttttaag gagtgtcgcc agagtgccgc gatgaaacgg gtattgttgg
     3661 tatacagaac ttagcatcga aggcctattg ttttcgacag gaggccatcg aaatgacact
     3721 atggtaccgt ctttacccac cgccttctgc taagtttagg aatacaagat gttgctgccg
     3781 ttaaaaaata gtcgtaattt ctattagaat ttgggcaata taaccagcac atagataagg
     3841 gagtcgtttt atgcacgaac tatttgtgct ggttttgagc acctgtgcta gcctcagcaa
     3901 tatgtcaggt tgttcagtgg aagtagtgaa tgtcaacacc acaaaagagc cggtaaacgt
     3961 cttttatagc aggaaagact gcgaagaaag catgaaaaac attatgctaa atcatgctct
     4021 ataccatgaa gtatcaggca gagagccata tatggcaaag tgcgaacaag tatttgtctc
     4081 aaaaagttta atgaaataat tggccaaaaa cgacatataa caaactctta taggagctta
     4141 acgtggaaga ttacttagtt tttggtttag gttatgaagg cgatataaag aacgatgaag
     4201 cagggcttga taagataagc gtggtaacga aatcggtagt gcgttctacc aattccaatg
     4261 aaccagtggt ttaccaagtg actgagttta aagaatttaa tgttgtgaga catcaagcgt
     4321 ttgatagtga atactacaac atagcattcg atgtcttacc atcccgcgtc cgtattgatg
     4381 ctgcaatccg taaatatcac cccaagaaat cctcctcagc ttagtaagct ttgattttcc
     4441 attatcaacc agcaataata atgtcctcgg agcctgaaca actccggtga cttctgcgct
     4501 aaacggggac gtttatgcgc acatacaatc caaactctct tctcccttca ctgatgcaga
     4561 aatgcacctg tggttctttg catccaacgt ttgacctctg cggaggtgaa gcgtgaacct
     4621 cccacaagac ggtatcaaat tgcatcgcgg taacttcact gctatcggcc agcagatcca
     4681 gccttatctg gagaacggaa aatgctttcg catggtgctt aaaccgtggc gtgaga
//
LOCUS       DAZKIP010000220         4714 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZKIP010000220 DAZKIP010000000
VERSION     DAZKIP010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.222"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..1905)
                     /locus_tag="ACHOUK_004661"
     CDS             complement(<1..1905)
                     /locus_tag="ACHOUK_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFP9899948.1"
                     /translation="MSQPAGDLVIDLSLDAARFDEQMARVRRHFSSLEADARKTASTV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIVTQLAGGQNPFLIMLQQGGQISD
                     SFGGPLSLLTLLKEELLGIRDASESSEESLSDTANALAENARNAGELGRFMSVARVAA
                     GGGVAVLAALAAAAWQAEQADRALLRSLILTGGAAATTTAELWKMAGVISDEAGGGIR
                     QAAENLARLAESGKYTAGQLRIMGETSQRWLQTVGDDAGKVEKAFEGIAADPVKALAS
                     LNQQYNFLSVSQLRHIDELERTKGKQVAVTEAMSLFADVMNARLEQLDKAATPVEKIW
                     DDVKTWTSDAWAWIGDHTLGALSLITDVVAGTVEQVKLLLVQGDLALAEFIQSAWETT
                     KNVPGVGALFGELAEENRVFIEKTKRDELALRKSIAERDARIRQGEMGYINRSRATGV
                     SKGPGQQEAVSRLAEELTGKKHTSPKTRSAGEREEEQAREALLALEAELRTLEKHSGA
                     NEKISRQRRDLWKAESQYAVLKEAATKRQLSEQEKSLLAHKDETLEYKRQLAELGDKV
                     EYQKRLNELAQQAVRFEEQQSAKQAAISAKARGLTDRQAQRESEAQRLRDVYGDNPAA
                     LAKATSALKNT"
     gene            complement(1886..2299)
                     /locus_tag="ACHOUK_004662"
     CDS             complement(1886..2299)
                     /locus_tag="ACHOUK_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFP9899949.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRRFYCTHYFQDTQLDAHFSGLMY
                     AVLSLFFGDPDMHPADFSLLAPACEEEQTEMPDEEEMLMQKATGVAGGVRFGGDGGRD
                     ISPSADVVDVSEDDVALMMASAGISGGVRYVPASG"
     gene            complement(2326..2748)
                     /locus_tag="ACHOUK_004663"
     CDS             complement(2326..2748)
                     /locus_tag="ACHOUK_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFP9899950.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRFDYIKFVSDAEQQETTKHD
                     VVHINQRYLETASLLVAMSLWHSHSLKGTLASPETEMQQIRREVMLGWPADALNQATN
                     RVLYLSGMLDNRHDADPEQTGKAEATESVTSKKHSKAS"
     gene            complement(2762..3514)
                     /locus_tag="ACHOUK_004664"
     CDS             complement(2762..3514)
                     /locus_tag="ACHOUK_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9899951.1"
                     /translation="MATPNPLEPVKGAGTTLWVYNGKGDAYANPLSDDDWQRLAKVKD
                     LTPGEMTAEPYDDNYLDDEDADWTATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFES
                     GDVRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSEITP
                     ATAIKVTPTSGTVAKGKTTTLTVSFEPESATDKTFRAVSADPSKATISVKDMTITVNG
                     VATGKVQIPVVSGNGQFAAVAEVTVTEAGAAG"
     gene            complement(3522..3917)
                     /locus_tag="ACHOUK_004665"
     CDS             complement(3522..3917)
                     /locus_tag="ACHOUK_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFP9899952.1"
                     /translation="MKHTDIRAAVLDALELHEHGATLFDGRPVVFDEEDFPAVAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAVTAIPALADLITTM
                     VTQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            complement(3914..4447)
                     /locus_tag="ACHOUK_004666"
     CDS             complement(3914..4447)
                     /locus_tag="ACHOUK_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9899953.1"
                     /translation="MAIKGLDQAIENLSRVRKNAIPAASAMAINRVATTAINQSSSQV
                     ARETKVRRKLVKERSRLKRATVRNPNARIIVKRGDLPVIKLGIRMLGRRPDSILKAGQ
                     HRYQRAFIQRLKNGRWHVMQRVAGKNRYPIDVVKIPMAAPLKQAFDENIDRIRRERLP
                     GELAYALKQQLRIAIKR"
     gene            complement(4462..>4714)
                     /locus_tag="ACHOUK_004667"
     CDS             complement(4462..>4714)
                     /locus_tag="ACHOUK_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFP9899954.1"
                     /translation="SGAVIRGVFDDPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRG
                     DTLTINGEMFWVDRVSPDDGGSCYLWLNRGQPPAVNRRR"
BASE COUNT          955 a         1494 c         1104 g         1161 t
ORIGIN      
        1 ggtgttcttc agtgccgatg tggccttcgc cagcgcagcc ggattatcac cgtacacgtc
       61 ccgaagacgc tgcgcttcag actcccgctg cgcctgacgg tcagtgagac cgcgggcttt
      121 tgcgctgatg gcggcctgct tcgcgctctg ctgctcttca aaccgcaccg cctgctgtgc
      181 cagctcattc aggcgtttct ggtattcaac cttgtcgccc agctcagcca gctggcgttt
      241 gtactccagc gtctcgtctt tatgcgccag cagggatttt tcctgctcag ataactgtcg
      301 tttcgtggca gcctctttca ggaccgcata ctgactttcc gccttccata aatcacggcg
      361 ctgccggctg attttctcat tcgcaccgct gtgtttttcc agcgtcctga gctcagcttc
      421 aagggcaagc agagcctctc ttgcctgctc ctcttccctc tccccggcag agcgcgtttt
      481 cggtgatgta tgctttttac ctgtcagctc ttcagccaga cggctgacgg cttcctgctg
      541 ccccggacct ttgctgacgc ctgttgcacg cgagcggttg atgtacccca tttccccctg
      601 gcgtatacgc gcatcccgtt ccgcaatgga ttttctcagc gccagttcat cgcgttttgt
      661 tttctcaata aatacgcggt tctcttctgc cagttcacca aacaacgcac caacgccggg
      721 cacattcttt gtcgtttccc aggctgactg aataaattca gccagcgcca gatccccctg
      781 cacaagcagc agcttcactt gttcaacggt tccggccacc acgtcagtga tcagactgag
      841 tgcccccagt gtatgatcac ctatccatgc ccatgcgtca gaagtccagg ttttaacatc
      901 gtcccagatt ttttccaccg gcgtggccgc tttatcaagt tgctccagac gtgcattcat
      961 gacatccgca aacagggaca tcgcctccgt caccgcaacc tgtttacctt tcgtgcgctc
     1021 aagctcatca atatggcgta actgggaaac gctcaggaag ttatactgct gattcaggga
     1081 ggccagcgcc ttcaccggat ctgctgcaat cccttcaaag gctttttcca ccttcccggc
     1141 atcgtccccc accgtctgca gccatctctg agaggtttcc cccatgatcc gtagctgccc
     1201 ggcggtatat ttcccgcttt ctgccagacg ggccagattt tctgccgcct gtctgatacc
     1261 accaccggct tcatcgctga tcaccccggc cattttccac aattctgccg ttgtggtggc
     1321 agccgcccct ccggtcagga tcagtgaacg caataaggcc cggtcagcct gctctgcctg
     1381 ccaggcggcg gcagcaagcg cggccagtac ggcaaccccg ccacctgccg ccacacgggc
     1441 caccgacata aatcgtccca gctcaccggc attccgggca ttttcagcca gtgcatttgc
     1501 cgtatctgac agcgactcct ctgatgattc agaggcatcc ctgatcccga gaagttcctc
     1561 cttcagcagg gtaagcaggc tgagcggtcc accgaatgaa tcgctgatct gccccccctg
     1621 ctgcagcatg ataaggaagg gattctgacc accggcaagc tgagtgacaa tatccgtgaa
     1681 ctgtgcgggc agtgtgcgca tggcagcctt atactgtccg actgatatcc cggctttttg
     1741 tgccgccagc gcctgtcggc tcagcccctg ttcaacagta ctggcggttt ttctggcatc
     1801 cgcctccaga ctggaaaaat gacggcgtac ccgggccatc tgttcatcaa accgggccgc
     1861 atccagactc aaatcaataa ccagatcacc cgctggctgg gacatatctc acacctccgg
     1921 aaatccccgc tgaagccatc attaatgcaa catcatcctc gctgacatcc accacatccg
     1981 cagaaggtga aatatcgcgc cctccgtccc ctccgaaccg gacgcctccg gcaactcctg
     2041 tcgctttctg catcagcatt tcttcctcgt ccggcatctc cgtctgctct tcctcacacg
     2101 ctggagcaag cagactgaaa tccgccggat gcatatccgg atcgccaaaa aacaggctga
     2161 gtacggcgta catcagcccg gaaaaatgag cgtccagttg ggtatcctga aaataatgcg
     2221 tgcagtaaaa acgtcgccag tcggcatatt cggtggatgt catcccggca agcatggcgc
     2281 gccagtcggg tctccccatc tctcgcgcca gtttcaggac aaagttcagc tcgccttcga
     2341 atgctttttt gatgttaccg actcagtcgc ttctgcttta ccggtttgtt caggatcggc
     2401 atcgtgccgg ttatccagca tacctgaaag ataaagcacc cggttcgttg cctgattcag
     2461 tgcatcagca ggccatccca gcatcacttc acggcggatc tgctgcatct ctgtctccgg
     2521 agaggccaga gtgcctttga gggaatggga atgccatagc gacatcgcca caagcaggga
     2581 tgccgtttcc agatatcgct ggttaatgtg cacgacatca tgcttcgttg tctcctgttg
     2641 ttctgcgtct gaaacaaact ttatataatc aaaccgctgc agcgcagaca attcggaaag
     2701 cgtgacggac acaccgttat attcaaattg ttctgttttc agaaacatgt attacctccg
     2761 tttaccctgc agcgcccgct tcagtaacgg tgacttcagc cactgcggcg aactgaccat
     2821 ttccgctcac cacagggatc tgcaccttac ctgtcgccac gccgtttacc gtaattgtca
     2881 tatctttcac actaatggtg gctttcgacg gatcggcgga aaccgctctg aacgtcttgt
     2941 cggttgcact ttccggctca aaagaaacag tcagggtggt tgttttccct tttgccaccg
     3001 taccggatgt cggtgtcacc ttaattgcag tggccggcgt aatttcgctg cgttcttccg
     3061 ccacggaagg tttgcccacg ttagtgactt tcaccgtgcg ggtgatcact tctttcgccg
     3121 tcacggcctt accgatactg ctgacccagc cacggaacac atccaccgtg ccatttggga
     3181 aacggatttt ataggcccgc acatccccgc tttcaaacca gcctataagc cctttctgac
     3241 cttcttctcc cggtttccag gccagcgtaa aactggtatc tcctgcagac ttctgcccct
     3301 gcccggtcgc ggtccagtcc gcgtcttcat catccaggta gttatcatcg tagggttctg
     3361 ccgtcatctc gcccggcgtc agatccttca ccttagccag tcgctgccag tcatcgtctg
     3421 acaacgggtt tgcataagca tcacccttgc cgttgtaaac ccacagagtg gtaccggcac
     3481 cttttaccgg ctcaagggga tttggtgttg ccatatcgtc ctcacatctc gtatgtaatg
     3541 gaataagtca gatccgcaga actccataac gccatatcgt catcacgacg atactcatag
     3601 ccctgcgtaa ccatcgtcgt aatcaggtct gccagtgccg ggatcgcagt caccgccgga
     3661 taaatccggc tttccatcca ctgatcaagc tccgaatccg gtacctgtgc cggtaaaaac
     3721 acctcaatat gcagtgtggc ccgccaggta tctgcatcca gctcttcacc ggtatactct
     3781 gcatccgtca gataaaccgc gacggccgga aaatcctctt cgtcaaaaac aacggggcga
     3841 ccatcaaaca gcgtcgcccc gtgttcatgc agctccagtg catccagcac tgccgcacgg
     3901 atatcagtat gtttcatcgt tttatcgcaa tcctaagttg ttgtttcagc gcgtatgcca
     3961 gttctccggg cagacgttca cgccggatac ggtcaatatt ctcatcaaac gcctgtttca
     4021 gtggggccgc catcgggatt ttcaccacat caatggggta acggtttttc ccggccacac
     4081 gctgcatgac atgccagcga ccatttttta atcgctggat aaatgcccgc tgataccgat
     4141 gctgaccagc tttaagtatg ctgtccggac gacggcccag catcctgatc cccagcttaa
     4201 tcacagggag atcaccgcgt ttaacgataa ttctggcatt cggatttctg accgtggccc
     4261 gtttcagtct ggaccgttcc tttaccagtt tccggcgtac ctttgtctcc cgggcaacct
     4321 gtgatgaaga ctgattaatc gccgttgtgg ccacgcggtt aatggccatt gctgaagccg
     4381 ccggaatggc gtttttacga acccggctca gattttcaat cgcctgatca agccctttta
     4441 tcgccataat tcaccctgcg tttatcgtcg ccggttaact gccggtggtt gcccacggtt
     4501 gagccagaga taacaactcc ctccgtcatc cggagaaaca cggtccaccc agaacatctc
     4561 gccgttaatg gtcagcgtgt caccacgccg cagttgccgc acatcatcag tccggacaaa
     4621 cagggacggg ctggagcctt caacgcgcac gccctgtccg gcatagctga tattttcagg
     4681 gtcatcaaaa acaccacgta ttactgcgcc ggac
//
LOCUS       DAZKIP010000221         4713 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZKIP010000221 DAZKIP010000000
VERSION     DAZKIP010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4713
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.223"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..327
                     /gene="cesT"
                     /locus_tag="ACHOUK_004668"
     CDS             <1..327
                     /gene="cesT"
                     /locus_tag="ACHOUK_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesT"
                     /protein_id="HFP9899955.1"
                     /translation="MMIYGVCGKFPTDNSNFALEILNANLWFAENGGPYLCYEAGAQS
                     LLLALRFPLDDATPEKLENEIEVVVKSMENLYLVLHNQGITLENEHMKIEEISSSDNK
                     HYYAGR"
     gene            387..3191
                     /gene="eae"
                     /locus_tag="ACHOUK_004669"
     CDS             387..3191
                     /gene="eae"
                     /locus_tag="ACHOUK_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin type gamma"
                     /protein_id="HFP9899956.1"
                     /translation="MITHGCYTRTRHKHKLKKTLIMLSAGLGLFFYVNQNSFANGENY
                     FKLGSDSKLLTHDSYQNRLFYTLKTGETVADLSKSQDINLSTIWSLNKHLYSSESEMM
                     KAAPGQQIILPLKKLPFEYSALPLLGSAPLVAAGGVAGHTNKLTKMSPDVTKSNMTDD
                     KALNYAAQQAASLGSQLQSRSLNGDYAKDTALGIAGNQASSQLQAWLQHYGTAEVNLQ
                     SGNNFDGSSLDFLLPFYDSEKMLAFGQVGARYIDSRFTANLGAGQRFFLPANMLGYNV
                     FIDQDFSGDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDIRF
                     NGYLPSYPALGAKLIYEQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVTMGIDYR
                     HGTGNENDLLYSMQFRYQFDKSWSQQIEPQYVNELRTLSGSRYDLVQRNNNIILEYKK
                     QDILSLNIPHDINGTEHSTQKIQLIVKSKYGLDRIVWDDSALRSQGGQIQHSGSQSAQ
                     DYQAILPAYVQGGSNIYKVTARAYDRNGNSSNNVQLTITVLSNGQVVDQVGVTDFTAD
                     KTSAKADNADTITYTATVKKNGVAQANVPVSFNIVSGTATLGANSAKTDANGKATVTL
                     KSSTPGQVVVSAKTAEMTSALNASAVIFFDQTKASITEIKADKTTAVANGKDAIKYTV
                     KVMKNGQPVNNQSVTFSTNFGMFNGKSQTQATTGNDGRATITLTSSSAGKATVSATVS
                     DGAEVKATEVTFFDELKIDNKVDIIGNNVRGELPNIWLQYGQFKLKASGGDGTYSWYS
                     ENTSIATVDASGKVTLNGKGSVVIKATSGDKQTVSYTIKAPSYMIKVDKQAYYADAMS
                     ICKNLLPSTQTVLSDIYDSWGAANKYSHYSSMNSITAWIKQTSSEQRSGVSSTYNLIT
                     QNPLPGVNVNTPNVYAVCVE"
     gene            complement(3455..4675)
                     /gene="escD"
                     /locus_tag="ACHOUK_004670"
     CDS             complement(3455..4675)
                     /gene="escD"
                     /locus_tag="ACHOUK_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscD"
                     /protein_id="HFP9899957.1"
                     /translation="MLSSYKIKLLNGAMRNRELQLPMGNLTLGTEDNDIVYFPLEQGL
                     NQFLLDIREEGVFLLSPVEFWIDGQPTPYEADKPLPVGKVIDIAGCCFIIGDIDHSLP
                     LSDVPERFSAKSRRKKRLILASVIGATFALSGAIGSYVLLSPKAEPPTFTRADVYQQL
                     KENKLHAITLVWHGKNIALYGRCESTTDLTPFFNYLKEKNIFYYNNIICNNQIISAIN
                     DVLTEYGYKDIIITKGNKPGFFLLSGYIPPSPKWSEVENLLLNTPGVAGWEIHNNSNN
                     KINELASEFKKNKLINYVNIFKKNDVIIVAGEVSQQNESKILAIINAMNKNSNAKILF
                     QNIQPYISADIFPGKILRISGTMKNPTIALDNGTSLGIGSILKGGYVIDAIDPKDGIN
                     ISRPDEYIHIPLSY"
BASE COUNT         1393 a          909 c         1020 g         1391 t
ORIGIN      
        1 atgatgattt atggtgtctg tgggaaattc ccgacagata actctaactt cgctcttgag
       61 attttgaatg caaacttatg gtttgcagag aatggtgggc catatctgtg ctatgaggct
      121 ggagcacaat cgctgttgtt agcgttacgt ttccctctcg atgatgctac ccctgaaaaa
      181 ctcgagaatg aaatagaagt cgttgttaag tcaatggaaa acctgtattt ggtattacat
      241 aatcagggaa taacattaga aaacgaacat atgaaaatag aggaaatcag ttcaagcgac
      301 aataaacatt attacgccgg aagataaaat ccgatctatt aatataattt atttctcatt
      361 ctaactcatt gtggtggagc cataacatga ttactcatgg ttgttatacc cggacccggc
      421 acaagcataa gctaaaaaaa acattgatta tgcttagtgc tggtttagga ttgttttttt
      481 atgttaatca gaattcattt gcaaatggtg aaaattattt taaattgggt tcggattcaa
      541 aactgttaac tcatgatagc tatcagaatc gcctttttta tacgttgaaa actggtgaaa
      601 ctgttgccga tctttctaaa tcgcaagata ttaatttatc gacgatttgg tcgttgaata
      661 agcatttata cagttctgaa agcgaaatga tgaaggccgc gcctggtcag cagatcattt
      721 tgccactcaa aaaacttccc tttgaataca gtgcactacc acttttaggt tcggcacctc
      781 ttgttgctgc aggtggtgtt gctggtcaca cgaataaact gactaaaatg tccccggacg
      841 tgaccaaaag caacatgacc gatgacaagg cattaaatta tgcggcacaa caggcggcga
      901 gtctcggtag ccagcttcag tcgcgatctc tgaacggcga ttacgcgaaa gataccgctc
      961 ttggtatcgc tggtaaccag gcttcgtcac agttgcaggc ctggttacaa cattatggaa
     1021 cggcagaggt taatctgcag agtggtaata actttgacgg tagttcactg gacttcttat
     1081 taccgttcta tgattccgaa aaaatgctgg catttggtca ggtcggagcg cgttacattg
     1141 actcccgctt tacggcaaat ttaggtgcgg gtcagcgttt tttccttcct gcaaacatgt
     1201 tgggctataa cgtcttcatt gatcaggatt tttctggtga taatacccgt ttaggtattg
     1261 gtggcgaata ctggcgagac tatttcaaaa gtagcgttaa cggctatttc cgcatgagcg
     1321 gctggcatga gtcatacaat aagaaagact atgatgagcg cccagcaaat ggcttcgata
     1381 tccgttttaa tggctatcta ccgtcatatc cggcattagg cgccaagctg atatatgagc
     1441 agtattatgg tgataatgtt gctttgttta attctgataa gctgcagtcg aatcctggtg
     1501 cggcgaccgt tggtgtaaac tatactccga ttcctctggt gacgatgggg atcgattacc
     1561 gtcatggtac gggtaatgaa aatgatctcc tttactcaat gcagttccgt tatcagtttg
     1621 ataaatcgtg gtctcagcaa attgaaccac agtatgttaa cgagttaaga acattatcag
     1681 gcagccgtta cgatctggtt cagcgtaata acaatattat tctggagtac aagaagcagg
     1741 atattctttc tctgaatatt ccgcatgata ttaatggtac tgaacacagt acgcagaaga
     1801 ttcagttgat cgttaagagc aaatacggtc tggatcgtat cgtctgggat gatagtgcat
     1861 tacgcagtca gggcggtcag attcagcata gcggaagcca aagcgcacaa gactaccagg
     1921 ctattttgcc tgcttatgtg caaggtggca gcaatattta taaagtgacg gctcgcgcct
     1981 atgaccgtaa tggcaatagc tctaacaatg tacagcttac tattaccgtt ctgtcgaatg
     2041 gtcaagttgt cgaccaggtt ggggtaacgg actttacggc ggataagact tcggctaaag
     2101 cggataacgc cgataccatt acttataccg cgacggtgaa aaagaatggg gtagctcagg
     2161 ctaatgtccc tgtttcattt aatattgttt caggaactgc aactcttggg gcaaatagtg
     2221 ccaaaacgga tgctaacggt aaggcaaccg taacgttgaa gtcgagtacg ccaggacagg
     2281 tcgtcgtgtc tgctaaaacc gcggagatga cttcagcact taatgccagt gcggttatat
     2341 tttttgatca aaccaaggcc agcattactg agattaaggc tgataagaca actgcagtag
     2401 caaatggtaa ggatgctatt aaatatactg taaaagttat gaaaaacggt cagccagtta
     2461 ataatcaatc cgttacattc tcaacaaact ttgggatgtt caacggtaag tctcaaacgc
     2521 aagcaaccac gggaaatgat ggtcgtgcga cgataacact aacttccagt tccgccggta
     2581 aagcgactgt tagtgcgaca gtcagtgatg gggctgaggt taaagcgact gaggtcactt
     2641 tttttgatga actgaaaatt gacaacaagg ttgatattat tggtaacaat gtcagaggcg
     2701 agttgcctaa tatttggctg caatatggtc agtttaaact gaaagcaagc ggtggtgatg
     2761 gtacatattc atggtattca gaaaatacca gtatcgcgac tgtcgatgca tcagggaaag
     2821 tcactttgaa tggtaaaggc agtgtcgtaa ttaaagccac atctggtgat aagcaaacag
     2881 taagttacac tataaaagca ccgtcgtata tgataaaagt ggataagcaa gcctattatg
     2941 ctgatgctat gtccatttgc aaaaatttat taccatccac acagacggta ttgtcagata
     3001 tttatgactc atggggggct gcaaataaat atagccatta tagttctatg aactcaataa
     3061 ctgcttggat taaacagaca tctagtgagc agcgttctgg agtatcaagc acttataacc
     3121 taataacaca aaaccctctt cctggggtta atgttaatac tccaaatgtc tatgcggttt
     3181 gtgtagaata attccataac caccccggct aaaatatgta ttgttttagt cggggcataa
     3241 ttatttcttc ttaagaaata acctcttata atcaaatcta ctactggtct ttttatctgc
     3301 ttaataggtc tctttcaaag agacacattc acgttttctc atgtaggttg atccaaccac
     3361 gctgatacca aagctgaatc acatcaagca acaactatgc tcacaacatc cacacaataa
     3421 aaaaccctcc gaagaggggg aagaggataa gaaattaata cgacagtgga atatgtatat
     3481 attcgtctgg gcgggagatg ttgataccat ctttaggatc aatcgcgtct atgacatagc
     3541 ctcctttcag aatgctacca atccccaggc tggtgccatt atcaagagca attgttggat
     3601 tcttcatcgt accgctgatt ctgagtattt ttccggggaa aatatctgct gaaatgtacg
     3661 gctgaatgtt ctgaaagagt atcttggcgt tactattttt gttcattgca tttataatgg
     3721 caaggatctt actctcattc tgttgagaaa cttcccccgc aacaatgatg acatcattct
     3781 ttttgaaaat attgacatag tttattagtt tattcttttt aaactctgat gccagttcat
     3841 tgattttatt gttactgttg ttgtgtattt cccatcctgc aactcctggc gtattcagta
     3901 acagattttc aacctcgctc catttgggtg aagggggaat gtatcccgat aacagaaaga
     3961 atccaggctt attacctttt gtaataatga tatctttgta accgtattcc gttaagacat
     4021 cattaattgc ggatatgatt tggttattgc aaatgatatt attgtaataa aaaatatttt
     4081 tttcttttag gtaattaaaa aatggagtta aatccgtggt tgattcgcaa cgcccataaa
     4141 gcgcgatatt tttcccatgc cataccagag tgattgcatg aagtttgttt tcttttagct
     4201 gctgatatac atcagctctg gtaaacgttg ggggttcagc ttttggggaa agcaaaacgt
     4261 aactgccgat ggcaccagac aacgcaaaag tagcaccaat cacgctcgcc aggataaggc
     4321 gttttttgcg tcgacttttg gcagagaacc tttctggcac atcagacaat ggcaaggaat
     4381 ggtcaatatc accaatgatg aagcaacatc ccgctatatc aataaccttt ccgacaggta
     4441 atggtttatc tgcttcatag ggcgtgggtt gcccatcaat ccaaaattca accggagata
     4501 aaaggaaaac accttcctca cgtatatcaa gtaaaaactg attgaggcct tgttcaaggg
     4561 gaaagtaaac aatatcgtta tcttccgtac ctagcgttag gtttcccatc ggaagttgta
     4621 attcccgatt acgcatcgca ccattgagaa gttttatttt atatgaggat aacattttta
     4681 aattttattc atcctggtgg ttgaacaatg aga
//
LOCUS       DAZKIP010000222         4512 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZKIP010000222 DAZKIP010000000
VERSION     DAZKIP010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.224"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..129
                     /locus_tag="ACHOUK_004671"
     CDS             <1..129
                     /locus_tag="ACHOUK_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HFP9899958.1"
                     /translation="SHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            472..1002
                     /gene="yodB"
                     /locus_tag="ACHOUK_004672"
     CDS             472..1002
                     /gene="yodB"
                     /locus_tag="ACHOUK_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFP9899959.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIVAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
     gene            1814..2587
                     /locus_tag="ACHOUK_004673"
     CDS             1814..2587
                     /locus_tag="ACHOUK_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899960.1"
                     /translation="MDITEFPSGVIEHLGWYVYRLIDPRDGSTFYVGKGKGNRVFAHM
                     RGEVAAADDDDLLSNKLKQIREIRLAGLEVIHVIHRHGMTDEKTAYEVEAALIDAYPG
                     LTNIMNGAGSNEFGAAHVKELIATYQPETITFHHKALMISVNRSAKDSELYDAVRFSW
                     RINVSRASKAEVILATVRGIVRGVFIADKWLKSTREHFPTMKYWDEDPDFEATQSSRY
                     GFEGREAPPEIANLYLGKKIPDELRKKGAMSPVRYSPNF"
     gene            complement(2645..3199)
                     /gene="pinE"
                     /locus_tag="ACHOUK_004674"
     CDS             complement(2645..3199)
                     /gene="pinE"
                     /locus_tag="ACHOUK_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
                     /protein_id="HFP9899961.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALNCAGCELIFEDKISGTKSERPG
                     LKKLLRTLSAGDTLVVWKLDRLGRSMRHLVILVEELRERGVNFRSLTDAIDTSTPMGR
                     FFFHVMGALAEMERELIVERTKAGLEAARAQGRIGGRRPKLTPEQWAQAGRLIAAGIP
                     RQKVAIIYDVGVSTLYKKFPAGDK"
     gene            3262..3723
                     /locus_tag="ACHOUK_004675"
                     /pseudo
     CDS             3262..3723
                     /locus_tag="ACHOUK_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308307.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            3723..4316
                     /locus_tag="ACHOUK_004676"
     CDS             3723..4316
                     /locus_tag="ACHOUK_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP9899962.1"
                     /translation="MKPVFDENGLATVPGDMRCFYYDAETSEYTGWSDEYINTGVSMP
                     ACSTGIDPGENIPGRVAVFTGKGWSHEEDHRNETVYSIENGAAVTVDYIGAIKDGYVT
                     ISPLTPYDKWDGEKWVTDTEAQHSAAVDAAEAQRQSLIDAAMASISLIQLKLQAGRKL
                     TQAETTRLNAVLDYIDAVTATDTSTAPDVIWPELPEA"
     gene            complement(4288..>4512)
                     /locus_tag="ACHOUK_004677"
     CDS             complement(4288..>4512)
                     /locus_tag="ACHOUK_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP9899963.1"
                     /translation="LIEITESERQLLINQANEYMNSKQWPGKAAIGRLKGDELTQYNL
                     WLDYLDALELVDTSGAPDIEWPTPPAVQAR"
BASE COUNT         1273 a         1002 c         1001 g         1236 t
ORIGIN      
        1 tctcattttc acatttttat gggtaatgat tcacagcaat ctttattaaa tgaaatggaa
       61 aactggccaa cgtattatcc atatcagttg agtagcgaag aagtggtcga ggaaatgatg
      121 tctcattgag tattctcatg ataacgcctc gatgccgctt tagtatgtca tcataactgc
      181 cactggtcca tccacaaacg ccactgaacg caagctagct acagacacgc tcatcactat
      241 gacgtgtctg tatattaata agctaacccg cattgagtta accaataacg gattccatac
      301 acaatacggc caacagagaa agatacatgt gctcacgcca ttgcttatat tggctggtta
      361 caatgtgcac tatcaatttt ttaaataaca aattcttaaa accacatctg tataaggaat
      421 ttttaaggtt cgtgggatag cttgactgtg aaaattacag gagctacaaa aatgaaccga
      481 ttctcaaaaa ctcaaattta tttacattgg ataacgctgc ttttcgttgc aataacctat
      541 gccgcgatgg aactccgtgg ctggtttcct aaaggtagta gtacttatct gctgatgcga
      601 gaaacacatt acaatgcggg tatattcgtt tgggtgttaa tgttttcacg cctgattata
      661 aaacaccgtt atagtgatcc ttctattgtg ccaccgccac cagcctggca aatgaaagcg
      721 gcttcgctaa tgcacatcat gctttatata accttccttg cgcttcctct gctggggatt
      781 gctttgatgg cttacagtgg aaaatcgtgg agtttccttg gtttcaatgt gtctcccttt
      841 gttaccccaa acagcgaaat taaagcactg ataaaaaata ttcacgaaac ctgggcaaat
      901 ataggctact ttttaatcgt agctcacgct ggcgcagcac tctttcatca ctacattcag
      961 aaagataata ctctgttacg aatgatgcct cgccgcaaat aagtcgatcc tctgacaaag
     1021 gaaatgtgaa ccatggacat caacgcatca taaatgttta cgcaagtgca taccgcactc
     1081 tttccactat caacaaggag gagcagaaaa tgtgtaaatc gtacaatggt aagacttatc
     1141 aaaaatacag gttaattttg gtatttaaac cttttttact tgttagtatg atacaaaggc
     1201 tttcaaaaaa gctgggaaat tcaacaactc ttggaaatta tggactttgt cccgcggctt
     1261 taatctgacg gccgcgttcc ttttttgact caattcttga ccccttctct acgcaacttc
     1321 agttcccacc accaactttg cggcagtttc ataggatgaa tgtctaaaag aataatggtg
     1381 agcgataaga aaacgactga ataactgcac attttcgctc gaaagcttcc tgtcagatcc
     1441 atagcgaatc aagtgctgaa tgtcacagta tcgaacagaa aacagtgacg atctaaccct
     1501 tcaagaatat tctacgattg ttctgtttag gaaaagcaag acgggaagtc gggagataag
     1561 tcattgataa agtggcggag agagggggat ttgaaccccc ggtagagttg cccctactcc
     1621 ggttttcgag accgacccga tcatcaaacg aaacataaaa ttagctcaca ttatgaggaa
     1681 aggtatcttt ttgcgctatg taaattcaaa gggttagcct cattttcccg atggctttct
     1741 caacactact agttgtgagc ccttgcaatg ttcattaata tacgtctcac aaataattca
     1801 cagatattgc aaaatggata ttactgagtt tccttctgga gtaattgaac accttggctg
     1861 gtatgtatac cgattgattg atcctaggga cggaagcacc ttctatgtag ggaaaggcaa
     1921 aggtaaccgc gtatttgccc atatgcgcgg tgaagtggca gcggctgatg atgacgactt
     1981 actgagcaac aagctaaagc aaatcagaga aataagatta gcgggacttg aagttatcca
     2041 tgtcatccat cgacatggaa tgactgatga aaagacggcg tacgaagttg aagcagcact
     2101 tattgatgcc taccctgggt taacgaatat catgaatggt gctggcagca atgaattcgg
     2161 cgccgcgcat gtcaaagagt tgatagcaac atatcaaccc gaaaccataa catttcatca
     2221 taaagcatta atgatttccg ttaacagaag tgcaaaagat tcagagcttt atgatgcggt
     2281 tcgatttagc tggcgcatta atgtctcgcg cgccagtaaa gcagaagtca ttcttgctac
     2341 tgtaaggggg atcgttcgag gagttttcat tgctgataaa tggctaaaat caacgcgtga
     2401 acattttcct acgatgaaat actgggacga agaccctgac tttgaggcaa cacaaagctc
     2461 tcgctatggt tttgaaggtc gagaagcccc acctgaaata gcaaaccttt atcttgggaa
     2521 aaaaatacca gatgaattaa gaaaaaaagg agctatgtct ccagttcgtt actcacctaa
     2581 tttttgaatc ttcaagtggt aagcataaac cgcagcacga tcttgcatac gacgtgctac
     2641 ggtttcattt atctccggcc ggaaacttct tatacagtgt cgatacacca acatcataga
     2701 tgatcgctac cttctggcga ggaattcctg ctgcaattaa tcgtccggcc tgcgcccatt
     2761 gttctggtgt aagtttggga cgacgtccac caattcgtcc ctgtgcgcga gcagcttcca
     2821 gtccagcttt tgttcgttca acaatcagtt ctcgttccat ttcagccagg gcccccatca
     2881 catgaaagaa aaagcgcccc attggggtac tggtatcaat agcatccgtc aggctgcgaa
     2941 aattaacgcc acgttcgcgc aactcctcaa ccagaatgac cagatgccgc atactgcgcc
     3001 ccagccgatc cagtttccag acaaccagag tgtcacctgc cgataatgtc ctgagcagtt
     3061 ttttcagtcc cggcctttcg gactttgtac cgcttatctt gtcttcaaaa atcagctcgc
     3121 atcctgcaca gttcagcgca ttacgttgta gatctgtgtt ctggtcattt gttgacacac
     3181 gtacatagcc aataagcatg gtagatctcc ctgacaaaag caggaatgat gccatttgct
     3241 cgttatttct gcattttcat aaacgttggt ttgggagaag gtgctccagc tattggcgtt
     3301 ccgttcttct ggccgtccgc tgcaatgcca aatactgtaa tcgacagctg gtcctgtatg
     3361 gtgtttttga agttcaacgg cgcgaaattc tctgccactg attatcctgt gctggcgaaa
     3421 gtgtttcctt cgctggtatt acctgaagcc cgcggtgatt tcattcgtat ctgggatgac
     3481 gggcgaggtg cagacggtgg tcgcgaatta ttaagctggc aggcagctac aaacttttct
     3541 cagtttgccg ggaatatagg cgaaggtgcg ggacacgcaa ttaactttca tgatggcatc
     3601 gccggaaatc agccaggatt ttcacgattt aatttcacca gtaactctgt gggtgatggt
     3661 gtgaattttg ttgcagtcag accgcgaaat attgcattta actttctggt gagggctaaa
     3721 taatgaaacc tgtttttgat gaaaatgggc tggctacagt gccgggcgat atgcgttgtt
     3781 tttattatga tgctgaaaca tctgagtata cgggctggtc tgatgaatat attaatactg
     3841 gcgtaagtat gcccgcctgt tccactggta ttgaccctgg cgaaaacatt ccgggaagag
     3901 tggcagtatt tacaggtaag ggatggagcc atgaagaaga ccatcgcaat gagactgttt
     3961 actcaatcga aaatggcgca gctgttacag tggattatat cggtgccatc aaagacggtt
     4021 atgtcacgat ttcaccgtta acgccatacg ataaatggga tggtgagaaa tgggtgacag
     4081 acactgaggc acaacacagt gccgcagtag acgcggcaga agcacagcgc caatcactga
     4141 ttgatgcagc aatggcttcc attagtctga ttcagctgaa gttacaggcc ggacggaaac
     4201 tgactcaggc agaaacaacc cgacttaacg ccgtgctgga ttacattgac gcggtgacgg
     4261 caacagatac cagcaccgcg ccggatgtca tctggcctga actgccggag gcgtaggcca
     4321 ttcaatatct ggcgcaccgg aagtatcgac cagctccagt gcgtccagat aatccagcca
     4381 caaattatat tgcgtcagtt cgtcaccttt cagacgacca atagccgctt taccgggcca
     4441 ttgcttactg ttcatgtatt cgttggcctg gttaattagt agctgtcttt ctgattcagt
     4501 aatttcaata ag
//
LOCUS       DAZKIP010000223         4353 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZKIP010000223 DAZKIP010000000
VERSION     DAZKIP010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.225"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(87..2420)
                     /locus_tag="ACHOUK_004678"
     CDS             complement(87..2420)
                     /locus_tag="ACHOUK_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HFP9899964.1"
                     /translation="MSGMKVSQAEKAARGHWSRILPALGVNVLKNRHQPCPVCAGKDR
                     FRFDDQEGRGTWFCNQCGAGDGLALVSKVLDVGISEAADRINGIIGNLLPVSQGMLES
                     GSPEKEDGRKAAAVLAARLFDKSRQTTGNAYLTSKGFPALPCRELTAMHKVGGVAFRA
                     GDLVVPLYADGELVNLQLINANGGKCFLKGGQVKNAFYLVEGTAKAAKRLWIAEGYAT
                     ALTINYLTGDAVMVAFSSVNFLSLASIACSEYPTHQIIIAADRDLNGAGQTRGAAVTG
                     ACNCTMALPPVFGDWNDAFTQNGEEATRHAIHEVIKPAVASPFDTMSEAEFTALSVSE
                     KAQRVVDHYKNSLAVDPNGQLLSRYEAGAWKVIYYADFARDVAALFQRLDAPFSSAKI
                     ASLVETLKLIVPQQQNPARQLIGFRNGVLDTRTGLFSPHDKKHWLRTLCEVDYTQPVD
                     GESLETHAPAFWRWLDRAAGFNPEKRDIILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     ILAEIATMLAGEDNATSATIEMLESPRERAALIGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDPKYQNAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVILHFPDQIAPEERD
                     TQLKEKIASELAVIVRQLMQRFSDPMSARTLLQSQQNSDEALTIKRDADSAFDFCGYL
                     EVLPDTTGMFMGNANIVPRQPRTYLYHAYLVYMEANGYKNTLSLTMFGKGLPLMLKEY
                     GLQYEKRRTNQGMQTNLALREESNADWLPKCDEFAAK"
     gene            complement(2434..2757)
                     /locus_tag="ACHOUK_004679"
     CDS             complement(2434..2757)
                     /locus_tag="ACHOUK_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 family protein"
                     /protein_id="HFP9899965.1"
                     /translation="MICCESLLALRAALYRRAVACAWLALSNHQERYSGLTLAELEDA
                     IARELEGFYLRQHGQQRGLEIACALLSDLMESGPLKACPVLSLLGMTVMDELCSRHLN
                     KPALH"
     gene            complement(2757..2978)
                     /locus_tag="ACHOUK_004680"
     CDS             complement(2757..2978)
                     /locus_tag="ACHOUK_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899966.1"
                     /translation="MNQFEISYDDVVRLKHLRNVGEYVTGMAALQDCYEKPAGAQCEQ
                     LVSLIYLMTEQLDGVVQRCHDDLMNAEVA"
     gene            complement(2975..3532)
                     /locus_tag="ACHOUK_004681"
     CDS             complement(2975..3532)
                     /locus_tag="ACHOUK_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFP9899967.1"
                     /translation="MMIPAYIKYLYSGLLTVVISRYSFSAVAKSAAGIGVPYNLLATI
                     DAPCVFFYVVAQAQPFSGLWCLCLHHGSIEIMVVRAGQPSGWPVSNKAGYANPVRAAT
                     SEIGVSGGSNNRYLLEAAIMATILTPSHPQYVFVFAAIRRADTHPRICMLRTVSCDER
                     SARRLLVRDYVLSLSARLPAGEVTL"
     gene            complement(3529..3789)
                     /locus_tag="ACHOUK_004682"
     CDS             complement(3529..3789)
                     /locus_tag="ACHOUK_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9899968.1"
                     /translation="MRNISVSAPAPAAPLFPVSNHHERFLRLPEVMHLCGLSRSTVYD
                     LISRDAFPKQIPLGGKNVAWAQSEVSAWMADRISARGRGCDA"
BASE COUNT         1105 a         1159 c         1049 g         1040 t
ORIGIN      
        1 catataactt ttaaatttta tcgatatata ttttgagata atttcctgta gcggatttaa
       61 gaccggcaca gccggtctta ggtcagttat ttcgctgcaa actcatcgca ttttggcaac
      121 cagtcagcat tgctttcctc tcttagggcc agattagtct gcattccttg attggtccgt
      181 cgtttctcat actgcagccc atattccttg agcattaacg gtagcccctt gccaaacatg
      241 gtcagactga gcgtattttt atagccgtta gcctccatgt agaccagata ggcatggtag
      301 aggtaagttc gaggctgacg tggaacaata ttagcgttcc ccataaacat gcccgtggtg
      361 tcaggtagga cctcaaggta gccgcaaaaa tcaaaagctg aatcagcatc acgtttgatg
      421 gtgagcgctt catcggagtt ctgctgtgac tgaagcaatg tcctggcact cattgggtcg
      481 ctgaaacgct gcataagctg gcgaacaatc accgctagct cactggcaat tttctctttg
      541 agctgggtat cgcgttcctc cggggctatc tggtcgggga aatgcaggat cacccttcgg
      601 cgtgaaactc caccactacg atcagtgaag cgcatcggat tattgttcac agccaggatg
      661 accgccggga tgtgggttga ataggcgttc tgatatttgg gatcaacgga taccgcatcg
      721 ccgccagtga tggcttttag tccggccccg tcaccgctcc acttttcctg gtcgggaagt
      781 cgaatcagtg aaaaacctat taacgcagct cgttctcttg gcgactcaag catttcaatg
      841 gttgccgagg tcgcgttatc ttcacccgcc agcatggttg ctatttcagc aagaatactc
      901 tttccacttc cgccagggcc agtgacctcc agaaacagct gccagtcata acggttagcc
      961 agcaccataa acaatgcagc cagaataatg tcccgttttt caggattaaa acctgcggca
     1021 cgatccagcc agcgccagaa tgccggggca tgggtttcca gtgactcacc gtcaacgggc
     1081 tgcgtgtaat ccacctcgca cagcgtacgt aaccagtgct tcttatcgtg cgggctgaac
     1141 aatcctgtcc gggtatcgag cacaccgttg cgaaatccga taagttgccg cgccggattc
     1201 tgctgttgcg gaacgatcag tttgagggtt tccacgagag acgcaatttt cgcggatgaa
     1261 aaaggtgcgt cgaggcgctg aaacagcgca gcgacatcac gggcaaaatc ggcgtaatag
     1321 ataactttcc aggcccccgc ctcatagcgt gaaaggagct gcccgttcgg gtctactgcc
     1381 agtgaatttt tatagtgatc cactaccctc tgcgcttttt cgctgacgct cagcgcggta
     1441 aattcagctt cgctcattgt gtcgaagggg ctggcaacag ctggttttat tacttcatga
     1501 attgcatgcc gggtggcttc ttcgccgttt tgcgtgaatg catcgttcca gtcaccaaac
     1561 acaggcggga gcgccattgt gcaattgcag gccccggtaa cagctgcgcc ccttgtttgc
     1621 cccgcaccgt tgagatcgcg gtcagcagca ataattatct ggtgcgttgg gtactcactg
     1681 caggcaatgc tcgccaggga aaggaaattg acggacgaaa aggccaccat gacagcatcg
     1741 ccagtcagat agttgatagt aagtgcggtg gcatatcctt ccgctatcca gagccgtttg
     1801 gctgctttgg cagtaccttc aaccaggtaa aaggcattct taacctgacc gcctttaagg
     1861 aagcatttgc ccccattagc gttgattaac tgcagattta ccagctctcc atctgcatac
     1921 aatggaacga caagatctcc cgcgcgaaat gccacaccac cgactttatg catagcggtt
     1981 aattcccggc aaggcagtgc aggaaaccct ttactcgtca gataggcatt gccagtggtc
     2041 tggcgggact tatcaaacaa acgggcagcc agcactgctg cagcttttct cccgtcctct
     2101 ttttcaggag aaccagattc aagcattccc tgagatactg gcagcaggtt tccgataatg
     2161 ccgtttattc tgtctgccgc ttcactaatg cctacatcca gtactttact tacaagcgcc
     2221 aggccatcac ctgccccgca ctggttacag aaccacgttc cccgcccttc ctggtcatca
     2281 aatcgaaagc ggtctttccc ggcacagacc gggcagggct ggtgccgatt tttcagtaca
     2341 tttacgccca gcgcaggtaa aattcttgac cagtgacccc gagctgcttt ctcagcctgg
     2401 ctaactttca ttcctgacat tgtgcggccc tccttagtgc agcgctggtt tgttgaggtg
     2461 acgggaacaa agttcatcca tgaccgtcat tccgagcagt gagagaaccg gacaggcttt
     2521 aagcggcccc gactccatca gatccgaaag caacgcgcag gcaatttcca gccctctttg
     2581 ctgcccatgc tggcgcagat agaacccttc cagctcgcgg gctatggcat cttcaagttc
     2641 agcgagcgtc aggccggaat aacgttcctg atgattgctt agagccagcc aggcacaggc
     2701 aacggcacgg cgatacagag cggcacgaag tgccagaaga gattcacagc atatcatcag
     2761 gccacctccg cattcatcag gtcgtcatgg cagcgttgca ccactccatc cagttgctct
     2821 gtcatcagat aaatgaggga aaccagttgt tcgcattggg cacctgctgg cttttcataa
     2881 cagtcctgca gggctgccat gccagttaca tactcaccca cattgcgtaa atgtttcagc
     2941 cttacaacat cgtcgtagga aatctcaaac tggttcatag cgtcacctcc ccggcaggca
     3001 gccgtgcaga aagggagagt acataatctc ggactagcaa acggcgcgcg gaacgctcat
     3061 cgcaggagac agtgcgaagc atacagatgc gggggtgagt gtcagcacgg cggattgcgg
     3121 caaacacaaa gacatattgc gggtgtgacg gggtgaggat tgtagccatg atggcagcct
     3181 ccaataagta gcggttattg ctaccaccgg aaacgccaat ttcactggtg gcagcccgaa
     3241 cggggttggc gtaaccggcc ttattggaaa ccggccagcc cgaaggctgc cccgcccgga
     3301 ctaccattat ctcgatagaa ccatggtgta agcataaaca ccacagccct gaaaatggtt
     3361 gtgcctgagc tacgacgtaa aaaaagacgc aaggggcgtc aattgtcgcc aataagttat
     3421 acggaacgcc aattccggct gccgattttg cgacagcgga aaaactatac ctggaaatga
     3481 cgacagtaag caagccagaa taaaggtatt taatgtatgc aggtatcatc atgcatcaca
     3541 tccccgccca cgggcgctga tgcggtccgc catccatgcg ctgacctcag actgtgccca
     3601 ggcgacattt ttaccgccaa gcgggatctg cttcggaaac gcatcgcggc tgatcaggtc
     3661 gtaaacggtc gaacgggaca acccgcataa gtgcatcacc tcaggcaggc gtaaaaatcg
     3721 ctcatgatgg ttcgatacag gaaataatgg cgcagcagga gctggcgcag acacagaaat
     3781 attacgcatt tatctaccct ctttaatttc cacaacagtc cggatgaatt tatccgggtt
     3841 caggtagttc cttattatgt gtatataaac cacctgcgca cgcatttttt atttagcgtc
     3901 acatattgat tttatggaat ttaaagcccg attaaaccat ataaaatgac ataataaata
     3961 ccatgcctgc ggactataaa tacaggagca ttaataggaa ctaccattca cccagggttt
     4021 actatcctat gggtcattgc acctgatagt gctaacccca ccgccaatac agcaaggcag
     4081 tacaacttct acacgaggcc ttgcattgca ggccccaatt tccgggcata tttttgaatc
     4141 gcatccaact tataaacatt gggcctgcat aaaaaccttg atattaaata acatcacctt
     4201 aaatcgtaaa atatatccga atgaatccat aaagaaccgg acagatctag ttttataaaa
     4261 atcagtatga aatgcaacac catggccaga ggcgaaaatg ttattccccg gcaaaaacat
     4321 gcagtctatt tatgtgcctg ttgaatactc ctg
//
LOCUS       DAZKIP010000224         4217 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZKIP010000224 DAZKIP010000000
VERSION     DAZKIP010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.226"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..209)
                     /locus_tag="ACHOUK_004683"
     CDS             complement(<1..209)
                     /locus_tag="ACHOUK_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFP9899969.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="ACHOUK_004684"
     tRNA            complement(324..399)
                     /locus_tag="ACHOUK_004684"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="ACHOUK_004685"
     tRNA            complement(406..480)
                     /locus_tag="ACHOUK_004685"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="ACHOUK_004686"
     tRNA            complement(597..681)
                     /locus_tag="ACHOUK_004686"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="ACHOUK_004687"
     tRNA            complement(690..765)
                     /locus_tag="ACHOUK_004687"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="ACHOUK_004688"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="ACHOUK_004688"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFP9899970.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHOUK_004689"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHOUK_004689"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFP9899971.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVTVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHOUK_004690"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHOUK_004690"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFP9899972.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFDAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLSQFPTAPNYPQADGSVKLAAGWLIDQCQLKG
                     MQMGGVAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1083 a         1047 c          944 g         1143 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctggaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagcgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc gacacgatga agaaacagcc gaatggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctgggagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctctg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg tagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcactat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaagtc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttctgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagaca tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagctatta agactgccat gacgttgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgcga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgcg gtagaagagg tcagactacg caaataattt gcaggggagc taatgctccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagcaataa agcgccttgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccaa cttttcccag
     2281 cgcgaaagat aaggtgccaa tccttcttgt tcgaagagtt ccagcgccgc gcgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcgagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacgacgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccggtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agcttgcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gaccccggcg accacggcca gcctgctggt attctgcaac gcaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggtactgat tcgtggagtc aatcactggc
     2821 agcacagtta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 ataggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcctaacgt ttcacccaac
     3001 tgctcacctg agtgaaattc gccattcgct aacagcgcga ttaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc aaccccaccc atttgcatcc cttttagctg gcactgatcg ataagccaac
     3301 ctgctgccag ttttactgaa ccatccgcct gggggtaatt tggtgctgtt ggaaattgtg
     3361 acagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcatcaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcgg tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgccct tctgcggttg catgctgcca ggcattgagt agttgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagtt atgacgacag ggagagtttg tagaaacgca aaaaggccca
     4201 gtcttccgac cgggcct
//
LOCUS       DAZKIP010000225         4208 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZKIP010000225 DAZKIP010000000
VERSION     DAZKIP010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.227"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..158
                     /locus_tag="ACHOUK_004691"
     CDS             <1..158
                     /locus_tag="ACHOUK_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9899973.1"
                     /translation="KSGLSSKGSKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFL
                     IEVKNER"
     gene            148..477
                     /locus_tag="ACHOUK_004692"
     CDS             148..477
                     /locus_tag="ACHOUK_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9899974.1"
                     /translation="MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAK
                     VKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSKWERNEIKPSGPALRILNTLAIK
                     GPEVFAK"
     gene            complement(547..1875)
                     /gene="dgcI"
                     /locus_tag="ACHOUK_004693"
     CDS             complement(547..1875)
                     /gene="dgcI"
                     /locus_tag="ACHOUK_004693"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFP9899975.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(1883..>4208)
                     /gene="pdeI"
                     /locus_tag="ACHOUK_004694"
     CDS             complement(1883..>4208)
                     /gene="pdeI"
                     /locus_tag="ACHOUK_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFP9899976.1"
                     /translation="KIRLIILFLLAALSFIGLFFIINYQLVSERAVKRADSRFELIQK
                     NVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLF
                     SRRANDKIVVYHQEQVNGPLVDESGQVIFADFNPSKRPWSVASDDSNNSWNPAYNCFD
                     RPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLFLKQGHEII
                     AKNQLAREKLIIYNSEGDYNIIDSVDTEYIEKTSAVPNNALFEIYFYYPGGNLLNASD
                     KLFYLPFAFIIIVLLVVYLMTTRVFRRQFSEMTELINTLAFLPDSTDQIEALKIREGD
                     AKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQYLAVGIIK
                     LCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCRENVQTFTR
                     KIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDHMFSEFIFC
                     DDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCRWVSAERGI
                     ISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVSPWQLNEP
                     HFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGLRISIDDFGTG
                     LSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVESEDVA
                     RELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
BASE COUNT         1261 a          961 c          824 g         1162 t
ORIGIN      
        1 ccaagagtgg tctaagcagt aaaggaagca aagaaatcga agatgatgaa ctggcagcct
       61 acaaaaaaat ggcaaatgca tttctggcct ttagcaataa gcaaatagag gatttgatcg
      121 aaactggctt cttgattgag gtgaaaaatg agcgataaat tacaaatgat gctggaagat
      181 gcaaaggaac tgaatgcact gggcctgatg tctgatagtg aggttgatgc cattgccgtc
      241 atggtcaaag cccgtgaagt cagcgcccgt atagccaaag tcaaatcaat gacgggtgaa
      301 gagatcaaag ccatccgcac ccgatacggc atgtcacaat ccgtgctggc acatacaatg
      361 ggaatgtcaa aagaaagtgt ctcgaaatgg gaacgtaatg aaataaaacc gagcggcccg
      421 gcactacgta ttttgaatac attggcaata aaagggccgg aagtttttgc aaagtaaaat
      481 gtttgcaaat gatttaccac attcattagt accccttgat tcaggggtac taacactatt
      541 ctaattctac tgccagctaa tctgagccct gccgtttttc ttactcttat acagccgctc
      601 atccgccagc attttcaagc gttcgacggt agtacattca tttgaatgcg ctacaccgat
      661 actggtgctt aaattaatgc ttcccacctg gaaatgaacg atttctttta gaatcgatag
      721 taactcttcc agcgtgccgc cagtataaat caccgcaaat tcgtcaccgc cgaagcggta
      781 gactttacct ttatctaccg ggatctgtga tttaaggatt ttcaccactt tcatcaacac
      841 ttcatcgccc agcaaatgac cgaaggtgtc gttaatattt ttgaatttat cgacgtcaaa
      901 caggcacaaa taaccgcctt tttccggcag cgcttttaaa tcttcgtcaa aggattttct
      961 acccattgct ttggtcaggt cgtccaggaa cgccgagtta tacatactgt agagtttttt
     1021 gacatcggca gccgaaaaga aaagcccgcc atccagtacg cgttcaaaat ggttattaat
     1081 aatggcttct ttctccacaa taccacgatt aatcactctg cgtaccagat agaggcaact
     1141 ggcacccgat atcactgcca ccaatgcggc aaagaaaata atcaatgtac agacccgcaa
     1201 ataaacatct tttttatacg caaatacgta caatccggtc ggcaaatagg cgaagtgctc
     1261 catcgcttcc gaaacggtga agatggcgtt tttagcatta tattcatcac cggtcaccgg
     1321 tttaccgttc ttaaacagag agaacatcag gttctcgctg tccatcgcca gcgattttga
     1381 cagtaaagaa aaacgctcca gaccaagcaa aaatatcaga ctacgattgt gagttaccgg
     1441 atagaggtaa tagacgaagg ttttaccgtc aaaagagata aaccctgtat ttttgacatg
     1501 tcgatacttc acaacctgca taatatctgc acaataattt ttctcgaact cctttacctg
     1561 aatacattcg tcactggaaa tcagataagg accgacatcg tacaaataag agggtgaaac
     1621 cgagtcggcg taagctgtgc gtaagccaac caacagctgt tcctccgtgg gatttagcgt
     1681 gttgataaga tcaattgcca cgctacgccg atattcagca atatttgcca ccgcaaaagc
     1741 agtatcaata acggactgtt tcaggctata cacccgttcc tttaccattt gagtgtagat
     1801 cccgcaggca actgctgaaa tcatgataaa aatcagcaga ctgactgtaa gtacgaactt
     1861 attgattctg gacatacgta aactactctt ttactaattt tccactttta tcccaggcgg
     1921 agaatggcat gggtttctga tacagatacc cctgagcctg aacacatcct aatttgatta
     1981 attcgcgcgc aacgtcttcg ctctctacgc cttccgcaat caccgggatc cggttatagc
     2041 gtgccaggtc gaaaataatg cgcacaattt ttcccgcagt accgaaaata tcgccggtaa
     2101 attgcgagtc cacctttatg ctgtctggat taatttcata aaaacgtttc aggtttgaca
     2161 aaccggtgcc aaagtcatca atcgatatcc gtaacccttg tttacgcagt tgttcaatat
     2221 tgagataaaa atgttcatta attcgctcga tcacggtttc agtgatctca acacagaggc
     2281 tgttcgcctt caggccattt tctttcatga tggtggtaaa acgctcatga aagtgcggtt
     2341 cgtttaactg ccagggcgaa acattaatat gcagtaagaa atcatccttc agcgatgcac
     2401 gctgactaaa atgtctgaat tcacccattg ccgttttaat aatctgataa cccagctcat
     2461 tgataaaccc gatatcttca gcaatggtaa tgaacttcag cggtgaaatg atcccccgct
     2521 ccgcagacac ccaacggcac aacgcctccg cgcccagaat ggcgcgggtg ttaatatcaa
     2581 cgatcggttg atagacgagg aatagttcgc caatttccat tgcatggcga atattacgcg
     2641 caatccagat attctcttct atttcgttga gtttcgcgtc atcacagaaa ataaactcgg
     2701 agaacatatg gtcacggata ctggaaatcg ccagtttcag tttttctgcg taactccagg
     2761 cgttttcccc ctgcaaaggt tcacaaatgg cagacttatg gatgcgcaga ttgcggtagc
     2821 caaagctgct gtcaaaatcg tttaccatcg ctattttacg ggtaaatgtc tgtacatttt
     2881 cccgacacag aagtaaatag agatcggcat tgaatgtcac gatatcgcag cattgtgcat
     2941 atttctcagc aattcgctga cacaatttcc tgacgatttt attgcgttca tcaacaccaa
     3001 acacagcttc cacggcttcc agaccacaca gtttgatgat ccccacagcc agatactgat
     3061 tattgttgga ctcaataatc gccatatttt taataaaacc actttcctga tcgtaagaga
     3121 tcagtgagag caatttattt gaccgttcaa tttcggcatc tttcatttcc gcgatcgaat
     3181 ttttgatgct gataatctct ttcgcatcgc cttcgcgaat tttcagagcc tcgatctgat
     3241 ccgttgagtc aggcaaaaac gccagcgtat taatcagctc tgtcatttca gaaaattgcc
     3301 gacggaacac acgagtggtc attaaataaa ccaccagcaa tacgataata atgaacgcaa
     3361 acggcagata aaaaagttta tctgatgcgt tcaataaatt accgcccgga taataaaaat
     3421 agatttcgaa taatgcgtta tttggcaccg ctgatgtttt ttcgatatat tcagtatcga
     3481 cagaatcaat aatattataa tcaccttcgc tattataaat aatcagtttt tcacgagcga
     3541 gttgattctt ggcaatgatc tcatggcctt gtttcaaaaa tagaacttca tcattagcgc
     3601 tgatttgatc aagatactcg ttcagatatc gccagtttaa atcgacatga attttatcca
     3661 ccgctaacaa atcgtgatct ttgccgttga tgcgtagcgt aaaagagata cattttttac
     3721 ccggacgatc aaagcaattg taggccggat tccagctgtt gttagagtca tctgaagcca
     3781 ccgaccacgg tcgtttcgat gggttaaaat cggcaaaaat aacctgccct gactcgtcga
     3841 caagcggtcc atttacttgt tcctgatgat aaacaacgat tttatcgttc gccctccgcg
     3901 aaaagagata atatttatta tcatcaagta ccagtcccac cgagtctaaa aatggcatca
     3961 tttccatttt caggatcaca gcgcgttgaa tctcctctgt attttttaat aaatacagtg
     4021 agtcctttaa tgtcagggcc gaacgttcaa tatctttaaa gaaatagcca acgtttttct
     4081 gaataagttc aaagcggcta tcggcacgtt ttaccgcgcg ttccgatacc agttgatagt
     4141 taatgatgaa aaaaaggcca ataaatgaca gtgctgccaa taaaaataaa attatcagcc
     4201 ttatcttt
//
LOCUS       DAZKIP010000226         4197 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZKIP010000226 DAZKIP010000000
VERSION     DAZKIP010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.228"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..223
                     /locus_tag="ACHOUK_004695"
     CDS             <1..223
                     /locus_tag="ACHOUK_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFP9899977.1"
                     /translation="NLSSPEGAVRLVTGSQQASIEIALDRVPANVSKIAITVVIDGED
                     TISSGCTLTDKMAIEKVQLTYLDLVVARW"
     gene            369..519
                     /locus_tag="ACHOUK_004696"
                     /pseudo
     CDS             369..519
                     /locus_tag="ACHOUK_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(737..2827)
                     /gene="iha"
                     /locus_tag="ACHOUK_004697"
     CDS             complement(737..2827)
                     /gene="iha"
                     /locus_tag="ACHOUK_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFP9899978.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQVRGSTQQRQGSSVTSL
                     SDTAATRIPYPTESQNYNLGARLDWKASEQDVLWFDMDTTRQRYDNRDGQLGSLTGGY
                     DRTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKRDKWGLAGQPRE
                     LKESNLILNSLLLTPLGESHLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDE
                     WHLTDALALTAGSRYEHHEQFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLH
                     KGISGVSGQGKTNLLGNPDLKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSY
                     SINDNTNSYVNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSY
                     TPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAW
                     TVVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYV
                     IPERNYWMSLNYQF"
     gene            2766..2948
                     /locus_tag="ACHOUK_004698"
     CDS             2766..2948
                     /locus_tag="ACHOUK_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899979.1"
                     /translation="MLLAENPRQGMTTEARVVIRILLISDHVNRQDNTQIQTIMRIIY
                     ICLLQKIKIIDKTKYK"
     gene            3607..4107
                     /locus_tag="ACHOUK_004699"
                     /pseudo
     CDS             3607..4107
                     /locus_tag="ACHOUK_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535762.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
BASE COUNT         1081 a         1031 c          935 g         1150 t
ORIGIN      
        1 taatttgtct tctcctgagg gggcagtcag gctcgttacc ggttcgcagc aggccagcat
       61 tgagatagca ctggatcgtg ttccggcgaa cgtcagtaaa attgcgatca ccgttgtcat
      121 tgatggtgaa gataccatca gttcaggctg tacgcttact gacaaaatgg cgattgagaa
      181 ggtgcaattg acttatttgg atttggtcgt ggcgaggtgg tgaccctgca ttctgctaac
      241 cgggttatga gtcaggagtc agcgatagac aaagggactt atctgaccgg gcccctggaa
      301 aaatggcggg agagaatgat accttcctgc tgacgagacc ggactttgag atgacgagcg
      361 atagtgcggc agtgatgtac agcctgatcg gtagctgcaa aacttaacgg aatcgagccg
      421 gaaacgtggc tacgccacgt gatcagtgta atcaacacct ggcctgccaa ccgcatgaaa
      481 gagttgttgc cctggaacgt cactctctct gtaaactaat ttttacccca cgtccttcac
      541 ggtgcgctta cactctggac aagtatgtaa atgataagtt aaaaataatc cgtgcatttc
      601 ctgtgacgat attagttgtt ggtaacgaaa tctcattagc ggatcggaaa tgcacttaac
      661 ctaatacgtt gccactgttc cgccaggagt tgcagtggca acgtattcta ccgtcagtga
      721 tagcgttttg ttattatcag aactgatagt tcagcgacat ccagtaattt cgctcaggta
      781 tcacatatcc tgttgttgat gatcccgtct ggaagtaatc accggcatac agcgtactct
      841 taccggcact gtacaggctc acgtcactgt aatccttgtt gagcaggtta ttcaccgcag
      901 cattcagcgt cagggcatcc gtcatcttcc acgacagacc tgcatccacc accgtccagg
      961 ctttcaggta ttctcctttc tcatcataca ctttcttctg tacagcgctc agtgacgaat
     1021 aattctgggt gaaacgtggt gttttcccgc gataacgggc acccagccat gatgccacct
     1081 cttcggtgat ctgccagttc agtttcgcat tcaccatgtg ttcaggggta taactcagcg
     1141 gcgcaccttt gttatcacca tcacgttgtt cacttcgggt ccaggtgtaa ttcagtgaca
     1201 gcgtgacatc ctctgaccac agcggcaatg tgccggcaaa ttccacaccg tgcaaccggg
     1261 cctttccgct gtttacatag ctattggtgt tatcatttat ggaataagag acaatcttgt
     1321 tggagaagtc agtcataaaa cctgtgacat tggcattcag accggcgggg ttatcgtaat
     1381 acaccccagc ctcataactg acgctctctt ccggcttcag gtcggggtta ccaagtagat
     1441 ttgtttttcc ctgcccggac acaccactaa tccctttatg tagctgcccc attctgggtg
     1501 ccttatatcc cgtggtcaca ccgcctttca gcgtccaggc atctgccaca tcccagacca
     1561 gatatgcacg cggactgaag tgtcccccga attgctcatg atgttcatag cggctgcccg
     1621 cagtcagcgc aagtgcatcc gtgagatgcc actcatcctc agcaaatacc gaccagcttt
     1681 tctgccggaa agtttcacct gtgctggcaa ggacaactcc gtctttcatg gacgagctct
     1741 gaaactcgcc ccccaccgta accagatgag attctcccag aggggtaagc agtaatgaat
     1801 tcaggataag gttcgattcc ttaagctccc gcggctgacc ggcaagcccc catttgtcgc
     1861 gcttcagtac actgcgtaca agctcacgac ctttattttc tgtctcgttc cagttcagat
     1921 acgatttcca tgttccgaag gtgaaagtat gatcatagcc agctgaaatt ttgtttcgct
     1981 catagcgcag ggtccggtca tatccccccg tcagactccc cagttgccca tcccggttat
     2041 cataacgctg ccgggtggta tccatatcaa accagagcac atcctgctcc gacgccttcc
     2101 agtcaagacg tgcaccaaga ttataattct gtgactccgt gggataagga atacgcgtgg
     2161 ctgctgtatc gctcagtgat gtgaccgatg aaccctgacg ctgttgtgtg ctaccgcgta
     2221 cctgcaggct gacagaatca tccacaaggg gaccactgct ccagaaatta aactggctgc
     2281 tgttacccca tttgttgctt tcctgcagat tcagccctgc attgacggaa gagagccatt
     2341 tgtctgcatt ctttctggta atgatattca ccacaccgcc catcgcatca gagccataca
     2401 gtgtggacat cggccccctg ataacctcaa tacgctcaat ggcggccaga gggggcatga
     2461 acccggtatt catggcagaa aaaccgttgg gagtcacgtc actgcttccg ccctgacgaa
     2521 caccatcaat cagtatcagc gtgtaactgg ctggcattcc tcggatgctg atttccagcc
     2581 ctccggtttt acccgtacca ctttcaacat ccacaccctc tactgatctc agagcctccg
     2641 ccagatcgtg gtactggctg gactgcaatt cctcctggct aatcacagaa acactggcgg
     2701 ctgcgttagt cagctttttc tcatagccgg atgccgagac aatcatcaca tcctctgcag
     2761 cagctatgct gctggctgaa aatccgagac agggaatgac tacggaagcc agagtggtta
     2821 ttcgcatatt actaatctcc gatcatgtta accggcaaga caatacacaa atacaaacaa
     2881 taatgagaat aatttatatt tgccttttac aaaaaataaa gattattgac aaaaccaaat
     2941 ataaataacg actgcctcgg catgccagtt aacagctatc cgtatggcgt gttcggtttt
     3001 gcgaataaaa aatggttact gtagaagcgg atttccacca catgtcaacc atgaatggtc
     3061 agtgatgcga gacgactggg atgatttggt cgatgacttc ttccggcgaa tactgataga
     3121 aacattgaaa tattgtaaga gaaagcgttc aaaattctgt ggcacgtgaa aaattctaca
     3181 gcgtcatcac cttattcagt cgaccttcaa aataaacgtt taacagagac aatcaccatt
     3241 tggattgtcc gctgaggagg cgaacaattt cctagacagc tttatcatgt cagcccacat
     3301 tgttggcgct ggcaatacta aggacaaacg atggggaagt agctttttag cgttattttc
     3361 gggagtaaaa aacgtggtat ctttccgtat gtcagttgta tcttgctggt attcgtagtc
     3421 gtgataaggc atacaacgat atttatcttg acgttagtga agaataagtt gttttgtgat
     3481 ctatgttaat ctgggattac aatccgggct tgatacatcg catcacgact tttattctct
     3541 tcagaataga atatgttact ccccgcttta caggctcagg atttgaatta caaattatac
     3601 ttttaagtga acaataacca agggtatttc ttatttcctg aaaccaggct tggcccattt
     3661 cgggatgagt ttgaaaaaac tcttgacgca tacgatagtg atgaaataaa cgaaacaaat
     3721 tacgtcaaca aactcaggcg tcttgcctga catgctcctg acatcattga tattcacgct
     3781 catctggcat atgcatttgc gctctttatg ctgcaattct ggcgaatgtt catttacagc
     3841 gtcatcagga tgccgttata ctcattgata aaatactaac atataaccct gagaataatc
     3901 agggaacacg ctggttactg ggggcggaat tgttacgtgc tggcaagcat ggcagggctc
     3961 tgtacgtatt gacaggacat gtcaatgaat ttccccccta ctggtacgag cttgctcttt
     4021 tacattttat gaatgaggca tatattaatg ctgcaacggc atttcgatat ggatttttaa
     4081 caagtatata ttgttgaaat gctttgttag aatctttatc cattccctag ggctgtatag
     4141 tatgattttt caggtatttc tgaatactgc caaatattat tgcaaaatat tctctaa
//
LOCUS       DAZKIP010000227         4134 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZKIP010000227 DAZKIP010000000
VERSION     DAZKIP010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.229"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..79)
                     /locus_tag="ACHOUK_004700"
     CDS             complement(<1..79)
                     /locus_tag="ACHOUK_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFP9899980.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTS"
     gene            complement(91..489)
                     /locus_tag="ACHOUK_004701"
     CDS             complement(91..489)
                     /locus_tag="ACHOUK_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFP9899981.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVGSAQPDTVILDTSELVTVVALVKLHTDALHVTRDE
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            complement(531..1556)
                     /locus_tag="ACHOUK_004702"
     CDS             complement(531..1556)
                     /locus_tag="ACHOUK_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFP9899982.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVREKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            complement(1612..1944)
                     /locus_tag="ACHOUK_004703"
     CDS             complement(1612..1944)
                     /locus_tag="ACHOUK_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFP9899983.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTSS
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            complement(1954..3273)
                     /locus_tag="ACHOUK_004704"
     CDS             complement(1954..3273)
                     /locus_tag="ACHOUK_004704"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFP9899984.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPGDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVPAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            complement(3254..>4134)
                     /locus_tag="ACHOUK_004705"
     CDS             complement(3254..>4134)
                     /locus_tag="ACHOUK_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9899985.1"
                     /translation="DGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKAMYAATIESE
                     LDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLMPGDSLNLQT
                     AQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANESWAYFMGRRK
                     FVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGSGRMAIDGLK
                     EVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPPAWAAAAFES
                     GLRQSTEEEKSDSRAA"
BASE COUNT          814 a         1292 c         1125 g          903 t
ORIGIN      
        1 ctgacgttcc catgtacccg cgtatcgttt catcggcgcg ggcaatggca gcatcgaaca
       61 ggttatcgaa atcagccaca gcgcctcccg ttattgcatt ctggccaggc cgcgctctgt
      121 catttcggct gccacaccgg cagagacacg aaacgccgtt cccggcagca caaatgccac
      181 aggttcatcc cgcgtgacgt gaagtgcatc agtatgcagc ttcaccagtg ccacgaccgt
      241 gaccagttca gacgtatcca gaatcacggt atccggctgc gctgatccca cctcattttc
      301 atgtccggtc agcacatttt cccggctgag aggggtgtcc tgaccggcag tttcatccgt
      361 gtcatcaagc tcctcttcca gctctgccac acggagcgcc agttcttctt tcgtccccgt
      421 caggctgaca tcacggttca gttgttcacc cagcgagcgg agacgggcaa tcagttcatc
      481 tttcgtcatg gactcctcca cagaaaaaca atggccccga agggccatga ttacgccagt
      541 tgtacggaca cgaactcatc agggtcagcc agcagcatca gcggtgctga ctgaatcatg
      601 gtgaactcac gcgccggatc gccggtggtc acccagtttt tcgggtaacg ggcagaggcg
      661 ttaatgcctt cgcgctgtgc gtccgcatcc tgaatgcagc cataggtgcg cagaccgcgt
      721 gcctgagtgt tccccagcac catcgtgttg tccggcagga agttcttttt gacgccgttt
      781 tccacgtact gtccggaata cacgacgatg gccacatcgc catacattcc cttataagac
      841 accgctttgc ccaggtcttt taccgctgtc tccagttcgg aatgagagcc gcgacgggta
      901 tccagcttct ccctgacggc tttgaaggaa cggaacagcg cccagccttt cgggtcaaac
      961 acgatgatat tcaccacgcc gctggcgttc agcgcgtagg cttcgatatc gtcggtcggg
     1021 tcatacgtgg acttgtcacg cttgctccac tccgtgccgc cggactgcgt gatgttgttc
     1081 gccgcactgc ggcccatatc cacctcaacc ggatcgaagg cttcaccggt catggtgtat
     1141 ttgcccttga gcacggcaga aactgcctgc atctcttcga cctgagcaat ggccagctct
     1201 tcgtcacgca tgttctgcat gatgatgcga cggcggcggt aagccgggtc cgccagattc
     1261 tgcggatctt catccggcag gcgacgcagg gtcatctgcg gattcacttc atgcttcggc
     1321 ttgacatatc ccggcgtaaa ttcagaggtg gagccgccac gggaacggat aacctcacca
     1381 gaaacaatcg gcgaaacgta cagcgccatg tttaccagtc ccggaatttg tgagagatag
     1441 actttctccg tggtgaaggg atagctctca cggaaaaaga gacgcagaaa cagcggatca
     1501 aacttaaatt tctgctcatt tgccgccagc agttgggcgg ttgtgtacat cgacataaaa
     1561 aaatcccgta aaaaaagccg cacaggcggc ctttagtgat gaagggtcag gttaaacgat
     1621 gctgattgcc gttccggcaa acgcggtccg ttttttcgtc tcgtcgctgg cagcctccgg
     1681 ccagagcaca tcctcataac ggaacgtgcc ggacttgtag aacgtcagcg tggtgctggt
     1741 ctggtcagca gcaaccgcaa gaatgccaac ggcagcaccg tcggtggtgc catcccacgc
     1801 aaccagctta cggctggagg tgtccagcat cagcggggtc attgcaggcg ctttcgcact
     1861 caatccgccg ggcgcggttg cggtatgagc cgggtcactg ttgcccagcg gctggtaatg
     1921 ggtaaaggtt tctttgctcg tcataaacat cccttacact ggtgtgttca gcaaatcgtt
     1981 aacggcatca gatgccgggt tacctgcagc cagcggtgcc ggtgccccct gcatcagacg
     2041 atccagcgca gtatcactgc gcgcctgtgc actctgtggt gctgcggcca gaatgcggcg
     2101 ggccgtttcc acggtcatac cgggggtttc tgccagcacg cgggcctgtt cttcgcgtcc
     2161 gtgagcctcc tcacagttga ggatccccat aatgcggctg ttttctgccg caaccgcagc
     2221 ggtgatctgc gcgttcacgt ccggctgcgc cgcgctggcg ttttcgccct ccgtcgctgg
     2281 caccacgcca gtaacgtcag cctgcgaagc agtggctgaa acagttgttg attgagtctc
     2341 tttggtcatt cgccctcctg agagacggga tttacgtgca tccagtgcat cacgcataac
     2401 ggtgatcgca tcggtgctgt tgacaagttc atcagccagt ccggcatcaa tggcctcctg
     2461 accgctgtac actgcagcct cggtatccag cacagcctgc acggacaggc cggtatatgc
     2521 cgacaccttc tgcgcaaaca tccggcgggt tgcatccatc cgggactgca gtgtttcccg
     2581 gacatcaccc ggtagatggc tgtaggggtt gccatccacc ttatggctgc cgctgtaaat
     2641 cagcgtgatt tccacgccct gtttctccag ggcagcaccg taattactgt gagccatcat
     2701 gacgccgatg gagcctgtcc gggcggtctg cgtgaccaga cgccgggagg cggcgctggc
     2761 aagcagctga cctgcactgc agttcatgtc gttggccagc gcccataccg gttttatgtc
     2821 tcgcacacgg gcgatgatgt cagcacagtc aaatgctccc gccaccatcc cgcccggtgt
     2881 gtccatatcg agcagaatgc cgtccaccat cggatcgctg gcagcctgtt gcagacgggc
     2941 gataatgccg ttgtaaccgg tcattcccga atacggctgc agcgcccgcg tccggctgac
     3001 cagcgtaccg gacaccggca gcacggcgat gccgttcatg acctgataac tccgggcctg
     3061 tcgtggtccg tcatcatcac cggataacgc cagcgccgcg ggtgcctctc cggcagtcag
     3121 gctgtcgccg gatactgcat ccgtcaggcg gctgatccca agctggcctg caagcgcaca
     3181 aaagaaaacc cgcgcatagg cgggttcaag catcagcggc tcattaaaag ccatgctggc
     3241 aatatgcggg agattacgca gctctgctgt cactcttctc ctcctctgtt gattgtcgca
     3301 gcccggattc aaatgccgca gccgcccagg cgggcggttt aagaccagct gcgcggcgct
     3361 ccatcgtttc acggacctgc tgggcaaaaa tttcctgata gtcgtcaccg cgtttcgcgc
     3421 actctttctc gtaggtgctc agtccggctt ctatcagcat caccgcttcc tgaacttctt
     3481 tcagaccatc gatggccata cgaccggagc ctatccagtc gcagttcccc caggcactgc
     3541 gggcttcctg aaagctgaag cgcgcttttg aaggtaacgt caccacgcgg cgaacgatgg
     3601 cctcttccag ccagcacaga aacatctggc tcgcctgacg ggatgcgacg aattttcgcc
     3661 gccccataaa gtacgcccac gactcgttcg cactggcccg tgccgtggag tagctcatct
     3721 gggcgtaatt ccgggaaagc tgctcatacg agacacccag cccggcagcg atataccgca
     3781 gcagtgactg ctcaaacacg gagtagccgt tatccgtgtc ctgagccgtc tgcaggttca
     3841 gtgagtcccc cggcatcagg tgcggcactt ttgcgcctcc cagacggacc ggtgctgcgg
     3901 cgtaatacgc ggcaatttca ccaatccagc cggtcagcct ttcccgctgc tcctgactgt
     3961 tcgcgcccag aataaaatcc atcgctgact gcgtatccag ctcactctca atggtggcgg
     4021 catacatcgc cttcacaatg gcgctctgca gctgcgtgtt ctgcagcgtg tcgagcatct
     4081 tcatctgctc catcacgctg taaaacacat ttgcaccgcg ggtctgcccg tcct
//
LOCUS       DAZKIP010000228         4095 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZKIP010000228 DAZKIP010000000
VERSION     DAZKIP010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4095
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.230"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            200..391
                     /locus_tag="ACHOUK_004706"
     CDS             200..391
                     /locus_tag="ACHOUK_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP9899986.1"
                     /translation="MSEDVPLPKVNQRYKDDHGALVTLTSVEETRVVFMQDGYPHPCM
                     RPMYNFLGKFKPEPREETE"
     gene            complement(444..677)
                     /locus_tag="ACHOUK_004707"
     CDS             complement(444..677)
                     /locus_tag="ACHOUK_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899987.1"
                     /translation="MARSDYDIINLSLEHELNEWLAERGYAGLVDNRNRLAEVVTRKL
                     QDSFYINVSRDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(773..1396)
                     /locus_tag="ACHOUK_004708"
     CDS             complement(773..1396)
                     /locus_tag="ACHOUK_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP9899988.1"
                     /translation="MRTRIDYLADKYCFTERNESPRLRRQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVVGNENTSLPYQQVAREVKAPRERLKYALEAGLLVTALD
                     GLFRFGSQRIAADVLRLRKAGMPVVTTTVEVHDNLTGTTRKVPAYHL"
     gene            complement(1485..1994)
                     /locus_tag="ACHOUK_004709"
     CDS             complement(1485..1994)
                     /locus_tag="ACHOUK_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9899989.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGIYGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWSVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSFM
                     SQVIATIIR"
     gene            2452..2911
                     /locus_tag="ACHOUK_004710"
                     /pseudo
     CDS             2452..2911
                     /locus_tag="ACHOUK_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3149..3846
                     /locus_tag="ACHOUK_004711"
     CDS             join(3149..3398,3398..3846)
                     /locus_tag="ACHOUK_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1F family transposase"
                     /protein_id="HFP9899990.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSPCRKTWQLQFT
                     YTASQPGKHQKIIDMAMNGVGCRASARIMGVGLNTVLRHFKKLRPQSVTSRIQPGSDV
                     IVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEV
                     VVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELH
                     DKVIGHYLNIKHYQ"
     gene            3863..4095
                     /locus_tag="ACHOUK_004712"
                     /pseudo
     CDS             3863..4095
                     /locus_tag="ACHOUK_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390635.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
BASE COUNT         1176 a          967 c          888 g         1064 t
ORIGIN      
        1 atgtttgacg attcaacagc atttaagtga gtttcaggat atgggaagga catgaaagga
       61 ggagcgcaat aaaatacaca ggaagaaggg gaaaatgttt cgtaaaaaca gaaaattaac
      121 agcgcacatc aggaaacgca gtctcctgat tcagtgctac cacatgaaaa tttagttaac
      181 tgttaccgta ctaaaattta tgtcagaaga tgttccatta ccgaaagtca atcagcgata
      241 caaagacgat catggtgcgc ttgtcaccct gaccagtgtt gaggaaaccc gggtggtgtt
      301 tatgcaggac ggctatccgc atccctgcat gcgaccgatg tacaactttc tgggaaaatt
      361 caaacccgaa ccccgggaag aaacagaata aaacgaaatc cctgccggca atcacactac
      421 ctgatagcag cataatccac ttattaattc tcatccccgg agacaagccc agaaaaccac
      481 tccgggtgtt cactgtatgc cgtgttcagc gcatccctgg aaacgtttat ataaaagctg
      541 tcctgcaatt tgcgggtaac aacttctgcc aggcgattcc ggttatccac cagtccggca
      601 taacctctct ctgccagcca ctcattcagt tcatgttcca gagacagatt gataatatca
      661 taatcggacc ttgccattcc cgtttcctta gcacatgttt tcaggcacgg ggataaagta
      721 taaagaatgt catactgctg ctgccagagc aatattcccc ctgatggttg tatcagagat
      781 ggtatgccgg tactttgcgg gttgttcccg tcaggttatc gtgcacctcc acggtcgtgg
      841 tcaccaccgg cattccggcc tttctcagcc tcagaacatc agccgcaata cgctgactgc
      901 caaacctgaa caggccgtcc agtgcagtca ccagtaaacc cgcctccagt gcatacttca
      961 gccgttcacg gggcgctttc acttcccggg caacctgctg atatggcaat gatgtgtttt
     1021 cattcccaac cacgcgacga atacggtgag acagatgata ccggtatgta tccggcacac
     1081 cagaaaggct ggccttcagg ctgtacacgc agccaaaccg tttatcgtta aacaccacat
     1141 ttttctggct gatgccccat tcttcacgca gcgcggcaat caattgcggt gtacgggtaa
     1201 gcagcaagcg aaaaggcagt tcaaaactgg tgacgtaatc cacattcagc agtgcaatac
     1261 gcaaccgttc ttctggcccg gcctctgtct gccggcactc ctccagaaca tcctgccact
     1321 gccggcgaag acggggggat tcgttccgtt ctgtaaaaca gtatttatcc gccagatagt
     1381 caattcgtgt acgcatactg aaaggaattc cgtataaaga ttcagcagac aaaactttat
     1441 cagccagtaa aaattaacgg aagcattgat attcctcccg acaatcaccg gatgattgtt
     1501 gcaataacct gcgacataaa ggactgaaca gccgttttta acgctacgta ttgccctgat
     1561 gaaccaggcc gggccacccg aaaacatctt tccacccggc attgtccgcc agcacttatc
     1621 accgaccagg tctgttgcag taaatccacc agttgcgcac attcttcatc agccatgacg
     1681 ccgactcgcg gatggcaaat actcgtctgg ctgagcagca acagacatcg tatctgtgct
     1741 ccttcatgtt gttcccgcaa ccagcgtaat accaccggcg ctgatgggct gaccgtgtga
     1801 ttatgctccg taatacgttc gtgtacccgt cggtgaaagg aactacaaaa tgtctgaatc
     1861 tgttgaccat agattccttc tgccgaatag agcgtgaagt gaaatgccaa cagatttggc
     1921 cagtggtcca ccgcctgctg aacaaaacgc cggatttccc ccggctctga aattaaggct
     1981 tcggttattt gcacgaaatg ttccttttca acccctgttg aattttgtca agccggtgca
     2041 gacacagtaa agcgcgcatc aacacctgaa aggtacgata caaacacaag ttacaaacaa
     2101 taagaacgga aggacaatta atcagtgagg aataataact aaattaacca aaagaatgtt
     2161 actgacaata ctaccaatac ttaaatagca atatattaat taatgtaaga acagatgccc
     2221 ggtaggacat atacacaata catccacaaa cacactcttt tcattaaatc aatcacctga
     2281 ttaaaccatg aaagtgtgat gtaagtcaaa attactgata ctatcaattg ataatgatta
     2341 tctctatcat tatcgtgaaa ttcgagaatg aaaggagaag tagtcattat aagcctttta
     2401 taaatcgtca tcatcatagt aaagtagaac actttttata tgagttacta tatgaaaata
     2461 tactggacaa gaaaacgtat tcctgaactt tcagcacttc caccttcatt aagaaagaaa
     2521 aattttactg acgcatataa tgcagcatct tctcatatcg aatactggat aggtgctggc
     2581 gtctcattta tctcgatgat gattttatcc agagtatatg actttctttt acccgcccaa
     2641 gatactttcc cgggtgatat catcagaagt ttatgtgttg tgtgtccatc catattaatc
     2701 tggtttcagt tttcggtata tgtcatgagg aaatattaca gacatattct ggtgcgagga
     2761 aaagaaaccg aaaccatttc agaaatggtt gatacgtgag gctgatacac gagaatatga
     2821 actatggcgt ccggtccgtc gctttttctc cattgttttc ctcattgttc tattaggttg
     2881 cattcattca ctgataacaa ctataaaata aaagttatgg ctgttctgat acaggaaaat
     2941 aaacgttcat tctatggtta cctcttcctg ttatatagaa gaattttaaa cgacaagtaa
     3001 gggaatataa atgaaaataa caaaaaataa aaatgcaact ataaaaaaca gtcatcagtc
     3061 ggctgctttt caatgtttac cttatcattt tctggtaatg ctgccaactt actgatttag
     3121 tgtatgatgg tgattttaag gtgcttgcgt ggcttccatt tccatcagat gtccttcctg
     3181 ctccgctact gaaggcgtgg tgcgtaacgg caaaagcact gccggacatc agcgctatct
     3241 ctgctctcct tgccgtaaaa catggcaact acagttcact tacaccgcct ctcagcccgg
     3301 taagcaccag aaaatcattg atatggccat gaatggcgtc ggatgtcgcg ccagtgcacg
     3361 cattatgggc gttggcctca acacggtttt acgtcactta aaaaactcag gccgcagtcg
     3421 gtaacctcgc gcatacaacc gggcagtgat gtgattgtct gcgctgaaat ggacgaacag
     3481 tggggctacg tcggtgctaa atcacgtcag cgctggctgt tttacgcgta tgacaggata
     3541 cggaggacgg ttgtggcgca cgtcttcggt gaacgcactc tggccacact ggagcgtctt
     3601 ctgagcctgc tgtcggcctt tgaggtcgtg gtatggatga cggatggctg gccgctgtat
     3661 gaatcacgcc tgaagggaaa gctgcacgtt atcagcaagc gttacactca gcgcattgag
     3721 cgacataatc tgaatctgag acaacatctg gcaaggctgg gacggaagtc actgtcgttc
     3781 tcaaaatcgg tggagctgca tgacaaggtc atcgggcatt atctgaacat aaaacactat
     3841 cagtaagttg gagtcattac ctgagaataa tacctcctgg gtaagtgagc agatactttc
     3901 agcgcctccg aaagatgtaa atattcgact tggagtcggt tctttagaag gtacaaccgt
     3961 ctcacatgtt cagcgattgc atcattcgtt aatcgcagct ggtttggaaa gtaacctcac
     4021 tgtctatacc ggtggtcacg attatgcctg gtggcgcgga gcaattattg atgaattagc
     4081 aaattataat tgcag
//
LOCUS       DAZKIP010000229         4090 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZKIP010000229 DAZKIP010000000
VERSION     DAZKIP010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4090
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.231"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..1114)
                     /locus_tag="ACHOUK_004713"
     CDS             complement(<1..1114)
                     /locus_tag="ACHOUK_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HFP9899991.1"
                     /translation="MATWQQGINSGGFLAGIGGQNSNAPKASDVSEALAYIRQNNEME
                     RSGRNNIGLQALQGLGSVAQTSQAAKQQEADAAFQKEYAAAIQSGDRQQVRDLMTKYP
                     GQLEKIQSGMKWADEDQRNSIGTLAAGARLAASSPEAMQSWLQNNAKELARVGVDPNN
                     VAQMYQQNPSGFGEFVYHLGMAALGPIDYFNVQDKMAGREIDRGRLAETIRSNQAGDF
                     EFKSKPSYLVLSFAEISSVVFILMSVYMVAKSQNSNGLQYDALFIPSMAFSILVFSFN
                     GGIISKIISNKVMILLGDASFSFYLVHTIVISTLSKFFNVSGLGAISVIKFIVMALFA
                     SLFISIMMYLFFEKPINNKLRKWWSGFKNDVSTTRVE"
     gene            complement(1124..1804)
                     /locus_tag="ACHOUK_004714"
     CDS             complement(1124..1804)
                     /locus_tag="ACHOUK_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFP9899992.1"
                     /translation="MLVLSESFKNKLLPMNGYMKGGSDSGSKAQARATEKGIELQREM
                     WQTNMQNLAPFTPLAQQYVSQLQNLSSLQGQGQALNQYYNSQQYKDLAGQARYQSLAA
                     AEATGGLGSTATGNQLAAIAPTLGQNWLSGQMNNYNNLANIGLGALTGQANAGQNYAN
                     NVSQLYQQQAAASAANANKPSGLQSFATGAIGGAASGAMIGSAVPVIGTGIGALAGGV
                     IGGLGSLF"
     gene            complement(1791..2258)
                     /locus_tag="ACHOUK_004715"
     CDS             complement(1791..2258)
                     /locus_tag="ACHOUK_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HFP9899993.1"
                     /translation="MEIKLIDNPVKLAEFLNNPVNTGNIVDSGDKYYIKPDAVYLGIY
                     EGLVLAGVHEVRNFWHSVVECHAVYDPGFRGEYALQGHRLFCKWLLENSPFLNSITMV
                     PDTTKYGRAIIRLLGATRVGHLDDAYMSNGKPVGITLYQLPRSKYEELLNVST"
     gene            complement(2258..2794)
                     /locus_tag="ACHOUK_004716"
                     /pseudo
     CDS             complement(2258..2794)
                     /locus_tag="ACHOUK_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail needle knob protein"
     gene            2858..3505
                     /locus_tag="ACHOUK_004717"
     CDS             2858..3505
                     /locus_tag="ACHOUK_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFP9899994.1"
                     /translation="MVNERSINGQAGKIVSKWVTLANNAIPSNVPPQWDLDLKGQQYS
                     EPTKCIGKQIDYMAKPETKLNDFNREASKSLYDWSDANVWGYSNGWARHEIKKETLSI
                     TGFHAVRRILKAVQASRKDNYKAKRQIQRTLKRQDINRSPITPFQAPLISKHDWDLSV
                     IAVTKEAETERRELLSSQIINSNKVQTHPLLRMLRRARVRASRAKRESGGYDHYY"
BASE COUNT         1186 a          938 c          909 g         1057 t
ORIGIN      
        1 tttcaactcg agtggttgaa acatcattct taaatccact ccaccatttt cttagcttat
       61 tgtttattgg cttttcaaaa aacaaataca tcattattga tataaataat gaagcaaaca
      121 gagccattac tataaattta attacactta tagcaccaag accagaaaca ttaaagaact
      181 tgctcaatgt actgattact atagtatgga ccaagtaaaa tgaaaaagaa gcatcaccta
      241 aaagtatcat aactttattt gatattattt ttgaaataat accaccatta aacgaaaaca
      301 ctaatattga aaaggccatt gatggtataa aaagcgcatc atactgtaat ccattgctat
      361 tttgactttt tgccaccata tagacagaca ttaggataaa gactacagat gatatttcag
      421 caaatgatag aactaaatat gacggctttg acttaaactc aaaatctccg gcctgattgc
      481 tgcggattgt ctctgccagc ctgcctcggt caatctcacg accagccatc ttgtcctgaa
      541 cattgaagta gtcaatcgga ccgagagcag ccatcccaag gtgataaaca aactcaccaa
      601 accctgaagg attctgctga tacatctgag caacgttgtt agggtcaaca ccgacgcgcg
      661 ccagttcctt ggcgttgttt tgcagccatg attgcattgc ttctggagac gaggccgcaa
      721 ggcgtgcgcc agccgctaag gtgccgatag aattgcgctg gtcttcgtct gcccacttca
      781 taccagactg aatcttctct aattgaccag gatatttggt catcagatct cgaacctgct
      841 gtcgatcgcc ggactggatg gctgccgcat attctttttg gaatgcagca tccgcttcct
      901 gttgctttgc ggcttgagat gtttgagcga cactaccaag tccctgcaac gcctgaaggc
      961 cgatgttatt gcgacctgaa cgctccattt cgttgttctg gcgaatatag gccaacgcct
     1021 cacttacatc acttgccttt ggcgcatttg agttttgccc accgatacca gcaagaaaac
     1081 cgcctgagtt gattccttgt tgccaagtag ccatattccc accttaaaac aatgatccaa
     1141 gaccaccgat aacaccgcca gcaagagcac caataccagt cccaataaca ggaactgcac
     1201 taccaatcat tgcacctgat gcggccccac caatggcacc tgtagcaaaa ctctgtaggc
     1261 ctgaaggctt attcgcattt gctgccgatg ctgccgcctg ctgttgatac aattggctga
     1321 cattgttagc gtagttctgt ccggcgtttg cctgacctgt aagagcacca aggccgatat
     1381 ttgccagatt gttgtagttg ttcatctgac ctgacagcca gttttgaccg agtgtaggtg
     1441 cgattgctgc taactggttt cctgttgctg tagagcctaa tccacccgtt gcctctgctg
     1501 ctgccagact ctgatagcgc gcctgccctg caaggtcttt atactgctga gagttgtaat
     1561 actggttaag cgcctgacct tgcccctgaa gagaggaaag attctgcaac tgtgatacgt
     1621 actgctgagc gagtggcgtg aacggtgcaa ggttttgcat gttcgtctgc cacatttcac
     1681 gctgcagttc gatgcccttt tcagttgcgc gtgcctgggc tttagatccg gagtcgctgc
     1741 cgcctttcat atacccattc atgggaagca atttattctt gaagctttcg ctaagtacta
     1801 acatttaata gctcctcata tttcgaacga ggtaattgat agagggtgat tcctaccggt
     1861 tttccgttac tcatgtacgc atcatcaagg tgaccaacac gggtagcgcc aagcaaacgg
     1921 ataattgccc gtccgtattt ggtggtgtca ggaaccatgg tgatgctgtt aaggaatggt
     1981 gagttttcga gaagccattt gcagaataat cgatgccctt gtagtgcata ttcgccacgg
     2041 aatccggggt catacaccgc atggcattca acaacgctat gccagaagtt acgcacttca
     2101 tgaactccag ccagcactaa tccttcgtag atgccgagat ataccgcatc aggcttgatg
     2161 tagtatttat ctccactgtc tacgatattc cccgtgttta ccgggttgtt aaggaattct
     2221 gcaagtttca ccgggttatc gatgagcttt atttccatca ctgctccgca atgattttga
     2281 tggttgtggc agtaaacgcc gcgccattcg actgaatggt taacgtgctg ccatttgtgg
     2341 caagaaagcc gtctttatcc acgctgaaga acgtagctaa caggatgttg tcggttgttg
     2401 ttgccgagtt gcgactgctt actagcgtgt caggaacaga accggagaat gttagctgca
     2461 ttgacctgtt ggcggttccg ctgggccacg tcccgacaat cgacagcttg aagaacaagg
     2521 ttttgttctc gttgaacaca accatcttgt tgttaacggt gtcgaagaat ggtgccaacg
     2581 tcccggatga cggcgtaagc gttttcagca ggctaacaag gttggtcggc gctgtcggga
     2641 tggttactga tacgccagag taaacaacct ctgacttctt gcgcgtagtg gcatactcca
     2701 gagcattaat gcgcgtttca tggtctgaaa cctgcgattc cagcgactga actctggtat
     2761 caagcgatgc aatatcgctt tcattcaagg caatcagtgg gtttacgtca tggagtgggc
     2821 tcaacaccag tcagagccgc atgtacacgc aataatcatg gtgaacgaaa gaagcattaa
     2881 tgggcaagct ggcaaaattg tcagcaaatg ggtaacactg gcaaacaatg caatcccatc
     2941 aaatgttcct ccccagtggg atctagattt gaaaggtcag caatattcag agcctacaaa
     3001 gtgtataggt aaacagattg attacatggc aaagcccgaa actaagttaa atgactttaa
     3061 tagagaggcg agcaagagcc tatatgattg gtcagatgcc aacgtttggg gttattcgaa
     3121 tggctgggcg cggcatgaga taaaaaaaga aacactttct atcactggct ttcatgctgt
     3181 aagacgcata ctaaaagccg ttcaggcatc aaggaaagac aactacaaag ctaagcgaca
     3241 gatacagcgt acattgaaac gacaagacat aaatcgatca ccaatcaccc cgttccaagc
     3301 accgttaatt agcaaacacg attgggattt atcagtgata gcggtcacca aagaggcaga
     3361 gacagagcgc agggaattac tatcctcaca gataataaac tcgaacaaag tacaaaccca
     3421 cccactgtta aggatgttaa gaagagcacg agtgagagca tccagagcca aaagagaaag
     3481 cggaggttat gaccattact actagtagtg acagatcgtg acaaggcggt gcaaaaaacg
     3541 accaataata agagagatat gcctgccaaa ccgccaaaag cgcggagcgc ggcaaacgcc
     3601 gcgagaggcg caagaaggcg aacctatcca ttgaaacggt cagaagcgca tcgcgctcga
     3661 cgaccgaagt ccccactaac accgttaacg aaagatcaaa gtcttagaaa aaaatgacgt
     3721 ttctttgatg gacttccatc cgtaaaaatc aaattttgag aaaaagtact tatagaaatt
     3781 aatcgctcta taaggataag ttgacgttag tttaaagtga taaaacatta aagaaaaaac
     3841 gtaaaatcaa atacataaaa taagtcacca agggatcatt ttcagaaaac aacgtcaacg
     3901 taacgtcaac tctgtaagaa cttttttggt cactttttgg gttgaatgta cgtaaaaatc
     3961 cccagcagtg aaatttgagc gcgtaagcac aattctgagg ggctttttgt ctgtaggtta
     4021 ggcagtgtaa ctaacaatac aagaagttag ctctgactaa ctatttaatt aacttttcaa
     4081 tctaattgcg
//
LOCUS       DAZKIP010000230         4083 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZKIP010000230 DAZKIP010000000
VERSION     DAZKIP010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.232"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            6..524
                     /locus_tag="ACHOUK_004718"
     CDS             6..524
                     /locus_tag="ACHOUK_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP9899995.1"
                     /translation="MADNHTLSAGYAQSKVQDFKNIKGVNLQYRYEWDSPVSVVGSFS
                     YMKGDWADSHRDEADDFYRHQADIKYYSFLAGPAYRLNDYISFYGLVGISHTKAKGDY
                     EWRNSVGADESDGYLSESVSKKSTDFAYAAGVIINPWGNMSVNVGYEGTKADIYGKHS
                     VNGFTVGVGYRF"
     gene            660..1451
                     /locus_tag="ACHOUK_004719"
                     /pseudo
     CDS             660..1451
                     /locus_tag="ACHOUK_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308008.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
     gene            complement(1456..2826)
                     /locus_tag="ACHOUK_004720"
                     /pseudo
     CDS             complement(1456..2826)
                     /locus_tag="ACHOUK_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(2846..3193)
                     /gene="tnpB"
                     /locus_tag="ACHOUK_004721"
     CDS             complement(2846..3193)
                     /gene="tnpB"
                     /locus_tag="ACHOUK_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9899996.1"
                     /translation="MITLPTGTRIWIIAGITDMRCGFNGLASKVQNTLKDAPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFAKRLERGRFVWPVTREGKVHLTPAQLSMLLEGIAW
                     QHPKRTERPGIRI"
     gene            complement(3190..3864)
                     /gene="tnpA"
                     /locus_tag="ACHOUK_004722"
     CDS             complement(3190..3864)
                     /gene="tnpA"
                     /locus_tag="ACHOUK_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFP9899997.1"
                     /translation="MNSQTKKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFIASGIAWPLPDSVSLAQLDAILYANRKKELTEPQISEGTWRKERRTSYSRE
                     FKIRLVKQALQPGAVVARIAREHGINDNLLLKWKSQYEDGLLSDDDIQECMPVPVALT
                     DTPEPTRPVTNPFWRNKPDECPESDPGNVPRCELHLKSGVVKLFDPLTPEMLRALIRE
                     MKGGTR"
BASE COUNT          917 a          981 c         1105 g         1080 t
ORIGIN      
        1 caagactggc tgataatcat acgctatcgg ctggttatgc tcagagtaag gtacaggact
       61 ttaagaatat taaaggagtg aatctgcaat atcgatatga gtgggattct ccggtcagtg
      121 tcgtcgggag tttttcgtat atgaaaggtg attgggcgga cagtcatcgc gatgaagctg
      181 acgattttta cagacatcag gctgatatta aatattattc atttctcgca ggcccagctt
      241 atcgtctgaa tgactatatc agtttttatg gtttggtggg gatatctcat actaaagcga
      301 agggagatta tgagtggaga aacagtgtgg gtgctgatga gtctgatgga tatttaagtg
      361 agtctgtatc aaaaaaatcc actgattttg cttatgcagc tggtgttatt attaatccgt
      421 ggggcaatat gtctgtaaat gttgggtatg agggaactaa agcagatata tatggaaaac
      481 attcggtcaa tggatttact gttggagttg gttatcgttt ttaatcatta gacgggactg
      541 gataattgca cttgcgtagt gaagtttact aaatgtttgt attttaatga agtattcgac
      601 atattccctt cagacggaat atgtcgacgg ttatggtttt actggagcat gagtatgtta
      661 tggcgaaact gataaatata tcagcgtcca ctgaggagag aatgacgcca ggggagagga
      721 gggtggcttc acgactggag agctttctga atgatgattg ctttgtctgg tacgatatcc
      781 cggtgggacg gaaaaaccga catccggatt ttgtcattat tgacccggat aacgggctgg
      841 tatttctgga ggtcaaggac tggactgtat ccacattacg taaagcaaat caggagcagg
      901 ttacactgga aaccgacggt cttctgaaat cagaaataaa tccgctggta caggttcgtc
      961 gttatgcctg tgatacggtt aatgccttac ctgctgaccc gtgcttacgg cagaatgacg
     1021 gtcagtataa ggggcggctg aacctggcct gggcgtatgg tgtggtgttt acccgcatta
     1081 cccgtcagca actgaaggca ctgacaggga ataatgagaa tgcggtggaa aagatttttc
     1141 cttcagcaca gacaatctgt caggatgaaa tgacgcagtc tgttttacct gaggtattcc
     1201 ggcaaaaaat tgccgggatg tttaccacgg ggttcagaac ccgggtaaca ccccggatga
     1261 gggatattct gcgcgcccat ctgtttcctg aagtgaccgt gaaacagaac tcacaaatca
     1321 aaattatgga tattcagcag gagattctgg ccagaaatat tggtgacggg catcgtgtca
     1381 ttcatggtgt ggccggttcc ggaaaaacca tgattctttt gttccgttgc ctttacctgg
     1441 cagaaaccac gtccggtagt tacgccgccc cagtgcaact acccggaggg cgttttcaca
     1501 caggttattg tcgatctcca cccagccatt gcggcagtat tcgttcagag cgtcccattg
     1561 cttcagcaga taggtgaacg ctttcgctgt atccgagtgg cgcgacagcg tgttcatctg
     1621 cccctggagc cactcataca acgactgcat tagcggtacc gttctggctt ttctgaccgc
     1681 cagtcgctct tctgccggac tgccgcggat ctcagcctcg atagcgtaca gttccccgat
     1741 acgacggagc gcttctcccg ttactgtcgt tggatggcgg acatgtacat cgtggatctt
     1801 gcgtcgggca tgtgccatac aagccgcttc ggttgcctga ccgctttcgt aaagagcatt
     1861 gtaacccgca tatgcatcgg cctgcaggat acctctgtag tccgccagat gttgctgtgg
     1921 gtggatgcct ttgcggtcgg gagagtatga gaaccacacc gctgccggca tggtcgaacc
     1981 ggcgttgcga tcgtcacgaa catagaccca cagccgtccg gtacgggttt taccctgacc
     2041 cggctccagt acatttaccg gcgtgtcatc ggcatgcact ttacccggca tcagcacata
     2101 gtgcttcagt tcatcataca gcgggcgaag ctgctctccc atgatgtcaa cccagcgccc
     2161 catcgtattg cggtgcagct ccacgccctg gcgggcatag atttccgact gacggtacag
     2221 cggcagatgc tcggcgaact tagccatgat tatgcgggcc agcagagccg gactggcgta
     2281 actgcgctcg atgggttttg gtggctgcgg agcctgaact atacagtcgc accggctgca
     2341 ggcccgtttt gggcgaaccg tttcgattac cctgaacgcg gtgttgatga tatccagttg
     2401 ttcagagatg ctttctccca gcggtttcag tttaccgctg cagacggggc attcggtttc
     2461 tgccggggag ataacctgcc tgtcacgggg aagtgttgcc ggaagtgctt tgcggacggg
     2521 agagtctgat gttttcggcg ctgtctctcc ggccattgag gtgagttgca actgcgcctc
     2581 accaagcctg ttctggagct cggttatacg cgtttctgcc cgtgcgatct tcttttctat
     2641 cttctcgcgg cttttctcgc tgctgcgacc gaacaacatt ctctgcagtt tagcgaccag
     2701 cgctctgagt gagctgatct cgcgggcata gccggttatt tcaccagaca gacggacgat
     2761 aacagcctgc tgtgcgatca gcagggcctt cagttgctcg atgttgtcgg ggagtgtgtt
     2821 gttcattccc ctgttttatc acgggttata tccggatgcc aggccgttct gtccgtttgg
     2881 gatgttgcca tgcgatcccc tccagtagca tggataactg agctggcgtc aggtgcactt
     2941 tcccttcccg ggttaccggc cagacgaagc ggccccgttc caggcgtttg gcgaacaggc
     3001 ataacccgtc acgatcggcc cacagtattt tcaccatttt gccactgcgg ccccggaaga
     3061 cgaagatatg cccggagaac ggggcatctt tcagcgtgtt ctgcaccttc gaagccaggc
     3121 cgttgaagcc acaacgcata tctgtgatgc cagcgatgat ccagattctg gtaccggttg
     3181 gcagcgttat catcgggtac ctcctttcat ttcgcggatt agcgcccgta acatttccgg
     3241 agtgagaggg tcaaacagtt ttaccacacc tgatttaaga tgcagctcgc accgtgggac
     3301 gtttccggga tcactctcag ggcactcatc aggcttgtta cgccagaagg gatttgtaac
     3361 tggtctggtc ggctctggcg tatcagtcag agccaccggg acaggcatgc attcctgtat
     3421 gtcatcatcg ctcagtaagc cgtcctcgta ctggcttttc cattttaaca gcaggttatc
     3481 attgataccg tgctctctgg cgatccgggc aacaacagca ccgggctgta atgcctgttt
     3541 taccagcctg attttaaatt cacgactgta ggaggttcgt cgttcttttc gccatgtgcc
     3601 ttcgctgatt tgaggctctg ttaattcctt ctttctgttg gcataaagga tggcgtcaag
     3661 ctgagctaat gaaactgaat cgggcaatgg ccatgcgata ccggatgcaa taaatcgctg
     3721 aaaaagcgta tgtattgtgg aatgactgag acctagacgc tgagcgatgg cccggatggt
     3781 cagtttatct tcaaatctta aacgcagagc atcaggcaaa taagaacgga agcagggaat
     3841 atcttttttt gtctgggaat tcatcgttcg tgtccatcta tatagatggg cgcgattgtt
     3901 gccagacagg acaattttca caagacgtcg cagatggggc gcttacacaa caatggcgcg
     3961 agggaaagct gctattacct tcttcagaga gcccccagct acttcctgtg actctcgatg
     4021 cagctgccga acagccagaa tcgctcgcag aggacccgga aaccctcagt atcagctgtt
     4081 gtc
//
LOCUS       DAZKIP010000231         4020 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZKIP010000231 DAZKIP010000000
VERSION     DAZKIP010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.233"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            167..883
                     /gene="nanC"
                     /locus_tag="ACHOUK_004723"
     CDS             167..883
                     /gene="nanC"
                     /locus_tag="ACHOUK_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFP9899998.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            903..2009
                     /gene="nanM"
                     /locus_tag="ACHOUK_004724"
     CDS             903..2009
                     /gene="nanM"
                     /locus_tag="ACHOUK_004724"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFP9899999.1"
                     /translation="MNKTITALAILMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLETQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLISHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAVDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNRLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2074..3054
                     /gene="nanS"
                     /locus_tag="ACHOUK_004725"
     CDS             2074..3054
                     /gene="nanS"
                     /locus_tag="ACHOUK_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFP9900000.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQSYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALVKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEAIYGNYQNNILANIIFVDFQQQGARGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSSAARRGIISDRFVEAILQFWRER"
     gene            3177..3872
                     /locus_tag="ACHOUK_004726"
                     /pseudo
     CDS             3177..3872
                     /locus_tag="ACHOUK_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT         1134 a          916 c          957 g         1013 t
ORIGIN      
        1 tgtggaaaga gatttttcac aggagacatt atacaaaaaa tataaaatac agctaccggt
       61 tgccaaagac actataagcc tggcaaaaaa atattacaca acataaatgc taattgttta
      121 tgcgggcttt gtattgcttt ctgtatccta caaatgagtg aaatttatga aaaaggctaa
      181 aatactttct ggcgtattat tactgtgctt ttcgtcacca ttaatttctc aggctgcgac
      241 actggacgta cgtggtggat atcgtagtgg aagccacgcc tatgagactc gactcaaagt
      301 cagtgaggga tggcaaaatg gatggtgggc aagcatggaa agtaatacct ggaataccat
      361 tcatgataat aaaaaggaaa atgccgcact caatgatgtt caggttgaag ttaattacgc
      421 gattaaactt gatgatcaat ggacggtgcg cccgggaatg ttaacgcatt ttagcagcaa
      481 cggcacacgc tacggaccct acgtaaaact gtcctgggac gcgacaaaag atcttaattt
      541 tggcattcgc tatcgttacg actggaaagc ttaccgacaa caagacttat ccggtgatat
      601 gtctcgtgat aacgttcatc gttgggatgg atatgtcact taccatatta atagtgattt
      661 caccttcgca tggcaaacga cgctatacag caaacagaac gattatcgct atgcaaacca
      721 taagaaatgg gcgacggaaa atgcatttgt tctacaatac catatgacgc ccgatattac
      781 gccatacata gaatatgact acctggaccg tcagggtgtt tacaacggca gagataattt
      841 atcggaaaac agttatcgca ttggtgtgtc atttaaactg tagtagacag gagacagtca
      901 caatgaataa aacaataacg gcgcttgcta tcctaatggc ttcatttgcc gcaaacgcgt
      961 ctgtattacc ggaaactcct gtaccattta aaagtggtac cggagcaatt gataacgaca
     1021 ctgtctacat tggtttaggt agcgcaggta cggcatggta caagctggag acacaggcca
     1081 aagataaaaa atggacagcg ttagctgcat tccctggtgg accaagagat caagcaacct
     1141 cggcatttat tgatggcaat ctgtatgtgt ttggcggcat tggcaaaaac agcgagggct
     1201 tgactcaggt atttaatgac gtacacaaat acaaccccaa aaccaatagc tgggttaaat
     1261 tgatatcgca cgcgccgatg ggcatggcgg gccatgtgac ttttgtacac aacggcaagg
     1321 cttatgttac tggcggtgtt aaccagaata tcttcaatgg ctattttgaa gatctcaacg
     1381 aagctggaaa agattcaacc gctgtagata aaatcaatgc acactatttt gacaaaaaag
     1441 cagaagatta tttctttaat aagtttctgt tgtcttttga tccctctaca cagcaatgga
     1501 gttacgctgg cgaatctccc tggtacggga cggctggtgc ggcggttgtg aataaaggtg
     1561 ataaaacctg gcttattaat ggcgaagcca aacccggatt gcgaacggat gccgtatttg
     1621 aacttgattt caccggtaat aatttaaaat ggaataggct tgctcccgtc tcatcaccag
     1681 atggcgtcgc tggcggtttt gcggggataa gcaatgattc tcttatattt gccggagggg
     1741 ccggattcaa aggttcacga gaaaattacc aaaacggtaa gaactatgcg catgaaggcc
     1801 tgaaaaaatc atatagcact gatattcatc tttggcataa cgggaaatgg gataaatcgg
     1861 gtgaattatc gcaaggtcgg gcctacggag tatcattgcc ctggaataat agtctattga
     1921 ttattggcgg tgaaactgca ggcggcaaag cggtgacgga ttcagttttg atctctgtga
     1981 aggataataa agtcacagta caaaactaac gcttcagggc cccggtaaag gggccctgct
     2041 atcaatttgt catttcaatt acgcgaaatt tatatgaacg caataatatc gcctgattat
     2101 tactatgttc ttactgttgc tggtcagtct aatgccatgg cgtatggcga aggactgcca
     2161 ttaccggaca gggaagatgc gcctcatccc agaattaaac aattagcgag atttgcgcat
     2221 acgcatcccg gaggcccgtc atgtcacttt aacgacatta ttccactgac tcactgccca
     2281 cacgatgttc aggatatgca gagttatcac catcctctgg caacgaatca tcaaacacag
     2341 tacggcaccg ttggccaggc actgcatatt gcacggaaat tactgccctt cattcctgat
     2401 aatgcaggga ttctcatcgt tccgtgttgc cgtggcggat cggcttttac cgcaggcagc
     2461 gaggggacat attcagaacg gcacggagcc agccatgatg cttgtcgttg gggaacggat
     2521 actccgctat accaggattt agtcagcaga acgcgagccg cactggtaaa aaatccgcag
     2581 aacaaattcc tcggcgtatg ctggatgcaa ggcgaatttg acttaatgac cagtgactac
     2641 gcgtcacatc ctcaacactt taatcatatg gttgaagcct ttcgtaggga tctaaaacaa
     2701 taccattctc agcttaataa tattactgac gcgccgtggt tttgcggcga taccacctgg
     2761 tactggaaag aaaatttccc tcatgcgtat gaagctattt atggcaatta tcaaaataat
     2821 attttagcta atattatttt cgtcgacttc cagcaacaag gtgcaagagg gctgacgaac
     2881 gcgcctgatg aagatccgga cgatttaagc acgggatatt acggttcagc gtaccggtca
     2941 ccggagaact ggacgacggc actgcgaagc agtcatttca gctcagcagc ccgtcggggg
     3001 attatttctg acaggtttgt agaagcaatt ttgcagtttt ggcgcgaaag gtgagcgttc
     3061 attaatttga tttattcgca tccatcagcg cttttaactc ctccggcgtg cggcactata
     3121 acgacttcca caacaaggag ccattttctt ataccacttt cagtgaccat ccgcttatgc
     3181 caccaccctc tacgcaactg tttgcagagc accttcccac cgagtggata cagcacttcc
     3241 tgacgctttc tgctcatgcg accgttcgtc gtcttaacct gagcgtggat ggcgaagcgg
     3301 ggatgaacct gctggcgccc gcagcgctgt cacccaggcg cgtcagcgcg tgggggccgc
     3361 cccggtggaa tggctcttcc gccagaccac acagacatgg ggcgcggaac gttacctgaa
     3421 ggatgactgg cacggcctgc aactttttgc cattgatggc gcacagttca ggacacctga
     3481 tgaacccgag ctgcgtgaat attatggctc tgccaacaca tccactgagc ggcagagcgc
     3541 ctaccccgtc atgcgtctgg tggcgttaat gaatctggga atcacattct actgaatgcc
     3601 atgaccgcgc ctaccggcgg agcgatacgg cgtgaggcaa caaaagcggc gtcagagatc
     3661 agctttaagt tcgcgttcca gtttatcgtg acagaaatga tagtgctggg aaacacggcg
     3721 tcaccgggga ctatcccgaa acgactggag catctgcggg ggctctggaa gtggtgttca
     3781 taacaaaacg cccccgacca tcaagggcga gggcggttaa gatatcaaaa acccgttatc
     3841 ctgtgaaaca cagtgccgcc ccgcttaagt gaacggcatt gtgtttctga gggaccttaa
     3901 ttttccctga ttctctgacg ttccttacca ttgatatttc cttcattcct caccagagaa
     3961 ataaaaaatc actatactca tcaaaatatg agattacctt tattcaggtg atcaccttac
//
LOCUS       DAZKIP010000232         4007 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZKIP010000232 DAZKIP010000000
VERSION     DAZKIP010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.234"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..185
                     /locus_tag="ACHOUK_004727"
     CDS             <1..185
                     /locus_tag="ACHOUK_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9900001.1"
                     /translation="TPDEQKEPVAAIAPSLSTSTQTRVSASSCKVEFRHGNMTLENPS
                     PELLTMLIRELSGRGR"
     gene            182..532
                     /gene="tnpB"
                     /locus_tag="ACHOUK_004728"
     CDS             182..532
                     /gene="tnpB"
                     /locus_tag="ACHOUK_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311895.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9900002.1"
                     /translation="MISLPSGTRIWLVAGVTDMCKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            563..2148
                     /locus_tag="ACHOUK_004729"
                     /pseudo
     CDS             563..2148
                     /locus_tag="ACHOUK_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            2177..2320
                     /locus_tag="ACHOUK_004730"
                     /pseudo
     CDS             2177..2320
                     /locus_tag="ACHOUK_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2431..2738
                     /locus_tag="ACHOUK_004731"
                     /pseudo
     CDS             2431..2738
                     /locus_tag="ACHOUK_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612262.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2735..3970)
                     /gene="pitB"
                     /locus_tag="ACHOUK_004732"
                     /pseudo
     CDS             complement(2735..3970)
                     /gene="pitB"
                     /locus_tag="ACHOUK_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
BASE COUNT         1053 a         1039 c         1071 g          844 t
ORIGIN      
        1 taacgccgga tgagcagaaa gaacctgtgg cggccattgc gccgtcttta tccacttcca
       61 ctcagaccag agtcagtgcc agttcctgca aggtggaatt ccgtcacggt aacatgacgc
      121 tggaaaatcc atcgccagag ctgctcacga tgttaatccg cgaactgagc gggaggggaa
      181 gatgatttca ctcccatcag gtacccgtat ctggctcgtt gccggcgtta ccgatatgtg
      241 taaatccttc aacggactgg gagaacaggt acaacatgtg ctgaatgata atcccttctc
      301 cggtcacctg tttatcttcc gtggccgacg gggggacacc gttaaaattc tttgggctga
      361 tgctgatggt ctgtgcctgt tcaccaaacg cctggaggaa ggccagttta tctggcctgc
      421 ggtgcgtgac ggtaaggtat ccattacccg ctcgcaactg gcaatgctcc tcgataagct
      481 ggactggcgt cagccaaaaa catcccgcct taacgccctg acaatgttgt aaaaaacacc
      541 tggccgcatt ataaaaacgg ccatgagtca gaaatacctc attcgcattg cagaactgga
      601 aagccagctc cgtcagaaag accagcaact gagtctggtt gaagagacgg aggccttcct
      661 gcactggcac gtgccgaaga aaagatcgaa gaagatgaac gggaaataga acatctgcgg
      721 gctcagatag aaaaactgcg ccggatgctg ttcggtaccc gttctgaaaa actgcgtcgt
      781 gaagttgaac aggctgaggc cctgctgaaa caacgcgaac aggacagcga tcgttacagt
      841 gggcgggaag acgatccaca ggttccccgc cagttgcgac agtcgcgcca tcgtcgtccg
      901 ttaccggcac accttccccg tgaaatacag cgtctggaat ctgaagaaag ctgttgcccg
      961 gagtgtggcg gtgagctgga ttatctgggg gaagtcagtg cagaacaact ggaactggtg
     1021 agcagcgccc tgaaagtgat ccgcacagaa cgggtaaaaa aagcgtgtac aaaatgtgac
     1081 tgcatcgttg aagcaccggc gccgtcccgc ccgatagagc gtggtatcgc gggccccgga
     1141 ttacttgccc gcgtgttaac gggaaaatac tgcgaacacc tgccactgta tcgtcagagt
     1201 gaaatctttg cccgacaggg tgtcgaactg agccgtgcat tactctccaa ctgggttgac
     1261 gcgtgctgcc agttaatgac gccgctgaat gatgccctgt accgttatgt gatgaatacc
     1321 cgcaagcttc acactgacga cacaccggta aaggtactgg caccgggcct gaaaaagacg
     1381 aaaacagggc gcatctggac gtatgtccgg gatgatcgca atgcgggttc gtcatctcct
     1441 ccggcggtct ggttcgcgta ctcaccgaac cggcagggga aacacccgga gcaacacctc
     1501 cgccccttcc ggggtatcct gcaggcggat gcgttcacag gttatgacag gctgttcagt
     1561 gcagaacgtg aaggtggtgc gctgacagaa gttgcgtgcc gggcccatgc ccggagaaaa
     1621 atccacgatg tatacatcag cagcaaaagt gcgacggcag aagaagccct gaagcgaatc
     1681 agtgaactgt acgccatcga ggatgaaata cggggattac cagagtcaga gcgtcttgca
     1741 gtcagacagc agcgaagcaa agcgttactg acgtcgctgc atgaatggat gatggagaag
     1801 aatggcacgc tgtcgaaaaa atccagactg ggcgaagcgt tcagctatgt actgaatcag
     1861 tgggacgccc tctgttatta cagtgatgac ggactggcgg agacggacaa taacacagcg
     1921 gaaagagcgc ttcgtgcagt ctgtcttgga aagaaaaatt acgtgttctt cggtagcgat
     1981 cacggcggcg agcgtggtgc actgctatac gggctgatcg gcacctgccg tctgaacggt
     2041 atcgatccgg aagcgtatct gcgccatatt ctgagcgtac tgccggaatg gccctccaac
     2101 cgggttgatg aactcctgcc atggaacgta gtactcacca ataaataagc gtcaatacgg
     2161 tgctccgttg acgcttacct tctttcattc gctgaaggtg gaatgtatcc acggggaacg
     2221 ctttagcagc cgggaaataa tgcgggaaac ggtgtttaat tatatcgagt gtgattacaa
     2281 tcgctggcgt cgtcacagtg cctgtggcgg tctcagcccg gactgtcttg cactgcaaat
     2341 attcagaaat gagtctatgc agttcaccag taaaatcagc catcagagag ggaatgtagt
     2401 ggcacagtaa atttggccac ctgattaaag gtgatatcct caccacaaca caaaacaggt
     2461 gacttaatga acaagaaaac caaacgtact ttcacccctg aattcaggct ggaatgtgca
     2521 cagctaattg ttgatgaggg ctactcatat cgacaagcca gtgaagcgat gaatgtcggt
     2581 tctaccacgc ttgagagttg ggtgcgccag ctcaggcgag agcgtcaggg gattgcgccc
     2641 tctgccacac ctattactcc agaccagcaa cgtatccgcg aactggaaaa gcaggttcgc
     2701 cgcctggagg aacacaatac gatattaaaa aaggcttctc accgatggtc atcgcaacac
     2761 gacgccagcc aatcatggtg ccaatgccga gcgccagtgc tactgccatg atgatccaca
     2821 ccggagcgta ctcaatggtg cttaacatat cgctgcgcag ttttttcagc aggttctggt
     2881 cttctttact gacgcctggc agtttcgcca gcttcgcgga agtatcagag atgcacagca
     2941 taatgcggcg cagctggctg cgctgactca cgcttaacgg ctcgtaactt tccatattgc
     3001 ctggcagcat cgttttaacg cgcgcaatcg catcaaaggt atttcccgga tgacagtgaa
     3061 actccgtcac ttgcgcgcca tcagtagatg ccgcaggcaa tggaggttcc atcgcaatca
     3121 acttctgcgg cagttcagga tgctgttgca ggtagtgttc gaagttggta acggcatcgc
     3181 gggtacgggt aatttcatag ccggacgcgt tcatattgac gacgaagcca gcaggggcaa
     3241 tccccaccag taccagcatc accaggccga tccctttttg tccgtcgttc gcgccgtgcg
     3301 aaaacgccac gcccgcagcg gaaacaatca gcgcaatacg cgtccagaat ggcggcttac
     3361 gtttgccttt tttctttttg cgatcttccg gaatgcggtg aatacggtca cgctttttcg
     3421 ttccgctcca gtagcgtcgc agcaggaata tcaggcctcc cgcaatgacc aggccgacga
     3481 taggggaaac aatcagcgag gagaaaattt tggtcacttc acgcaggttt aacgcatcca
     3541 tcaccgatga gccggttaac agcgcgttgg ttaaaccgat gccgataatc gcgccaatca
     3601 aggtatgcga actggaggcc ggtaaaccaa agaaccacgt tcccaggttc cagataatcg
     3661 ccgccagcag catggaaaag accatcgcca ggccgtgggt tgaccccata ttcagcaaca
     3721 aatcggttgg caacatatgg acaatggcat aggcaacgct aagtccgccc aataacacgc
     3781 caaaaaagtt aaaaaatgcc gccatcacca cggcaagctg tggctgcatg gcacgagtat
     3841 aaataacggt tgccaccgca ttcgccgtgt catgaaaacc attgattgct tcgtagaaca
     3901 acacaaatgc cagagcaagt aataacaaaa gccctgtgta tatatcaagg ccaacaaata
     3961 aatttagcat atattagatt acgccatttt gaatttacga acggacg
//
LOCUS       DAZKIP010000233         3939 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZKIP010000233 DAZKIP010000000
VERSION     DAZKIP010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.235"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(39..227)
                     /locus_tag="ACHOUK_004733"
                     /pseudo
     CDS             complement(39..227)
                     /locus_tag="ACHOUK_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            1407..3554
                     /locus_tag="ACHOUK_004734"
     CDS             1407..3554
                     /locus_tag="ACHOUK_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFP9900003.1"
                     /translation="MLYNIPCRIYILSTLSLCISGIVSTATATSSETKISNEETLVVT
                     TNRSASNLWESPATIQVIDQQTLQNSTNASIADNLQDIPGVEITDNSLAGRKQIRIRG
                     EASSRVLILIDGQEVTYQRAGDNYGVGLLIDESALERVEVVKGPYSVLYGSQAIGGIV
                     NFITKKGGDKLASGVVKAVYNSATAGWEESIAVQGSIGGFDYRINGSYSDQGNRDTPD
                     GRLPNTNYRNNSQGVWLGYNSENHRFGLSLDRYRLATQTYYEDPDGSYEAFSVKIPKL
                     EREKVGVFYDTDVDGDYLKKIHFDAYEQTIQRQFANEVKTTQPVPSPMIQALTVHNKT
                     DTHDKQYTQAVTLQSHFSLPANNELVTGAQYKQDRVSQRSGGMTSSKSLTGFINKETR
                     TRSYYESEQSTVSLFAQNDWQFADHWTWTMGVRQYWLSSKLTRGDGVSYTAGIISDTS
                     LARESASDHEMVTSTSLRYSGFDNLELRAAFAQGYVFPTLSQLFMQTSAGGSVTYGNP
                     DLKAEHSNNFELGARYNGNQWLIDSAVYYSEAKDYIASLICDGSIVCNGNTNSSRSSY
                     YYYDNIDRAKTWGLEISAEYNGWVFSPYISGNLIRRQYETSTLKTTNTGEPAINGRIG
                     LKHTLVMGQANIISDVFIRAASSAKDDSNGTETNVPGWATLNFAVNTEFGNEDQYRIY
                     LALNNLTDKRYRTAHETIPAAGFNAAIGFVWNF"
BASE COUNT         1169 a          859 c          817 g         1094 t
ORIGIN      
        1 aaccagcaaa cgggcattgt gttctgaaat ccaggctatt gattcaacgt cagcgacaga
       61 tctgcgctgt aaaagaactt gtaaaacaac gtaatagaat tatccggaat ggtggcgact
      121 atgcactaga acaccacaat ttcgctctct cggtaaggca ctgtcacagc attcaacaga
      181 atgtgacttg ccagatttat tagcaccacc agatgtttaa ccgggtaacc acgacccagt
      241 cggggatata ttccatcttc tcgctgacat catcaagaat aaaaaggttt attacactca
      301 gagcaacgtt cttcagcagg cgggataatt cttcaacatt tagaagatgt gttattaaag
      361 gcatacaaac tggacgtatt gtattttctt ttggtggttg atctaaggta gcagtttatc
      421 ctgatgcgct gagatttctg taatatctac gtcaacattc tcctcgaata gaaaatgcag
      481 ccccggcaag taattcacat tttttaacaa aacgttgtta gcgtgccatt ttcagaatct
      541 ctccaagaaa gcgaattctt ttctcaaatt ctgcattctc agacacagct ttctactcca
      601 tggtaatggc ccaaatacga agttgctcag gatcattagt ggtgcttaga agttaaataa
      661 ccatgcggag taccactcgt catcacgtat ttatatatca tgaagctatt gttgtgtttt
      721 gtacatcagt agatatattc tgatatactc cttttgctag acataacctt tcacctgctt
      781 gcaaagcttc tgtgttctga cattgccaaa ttgttgcaat tctgtatcca gccttctttc
      841 agtcatagct tcgggccgcg ataagactca ctgatctgac cctgattcct cttgcagact
      901 ttatagacca attaaaatgc agtttctgca ggtcaacgtc tgaccatcat tgtcatcact
      961 ctggccatta gagtaacctt ctgcattcat ccttttgtaa aaagtttata ttagtatcag
     1021 caattaaccg gacctgatac tgatatgagt cttaccgcat atacggtcaa tttcagcaat
     1081 taattacatt atccacgcca aagtatttgt catcacaatg atggtacctt ctttcagaca
     1141 ccattttttc aactccgttt tccacggacc gcactcttat gtcaagagtg cggtccgtgg
     1201 atacaaccag agaccgactg acacgagtca gaggaaacga cggatatgtt cagtcgtaaa
     1261 atatctatca aaaaacatga ttaaggtcaa aaatgtttga tatttacaat ttatgaagat
     1321 gacaataatt atagatatat gagaacataa atgaaaataa ttatcattac agcaatcatt
     1381 tgcactttgt attaatgagg gatgaaatgt tatataatat accttgtcga atttatatcc
     1441 tttccactct gtcattatgc atttctggga tagtttctac tgcaaccgca acttcttcag
     1501 aaacaaaaat cagcaacgaa gagacgctcg tcgtgaccac gaatcgttcg gcaagcaacc
     1561 tttgggaaag cccggcgact atacaggtta ttgaccaaca aacattgcag aactccacca
     1621 atgcctccat agccgataat ttgcaggaca tccccggagt agagataaca gacaactcct
     1681 tggcaggccg taaacaaatc cgcattcgtg gcgaagcatc ctcccgtgtt ttaattctca
     1741 ttgatggtca ggaggtaact tatcagcgcg ccggagataa ttatggtgtg ggactgttga
     1801 tagatgagtc tgcgctggag cgtgttgagg tagtgaaagg tccatattcc gtactgtacg
     1861 gttcacaggc aattggcggt attgttaact tcatcaccaa aaagggaggt gacaaacttg
     1921 catctggagt tgtgaaagct gtttataatt ccgcaacagc aggctgggaa gaatcaatcg
     1981 cggtccaggg gagcatcggt ggatttgatt atcgcatcaa cggtagttat tctgatcagg
     2041 gcaatcgtga tacgccggat ggacgtctgc cgaataccaa ctatcgtaac aatagtcagg
     2101 gtgtatggtt gggttataac tccgaaaacc atcgttttgg cctctcgctt gatcgctaca
     2161 gactcgcgac acaaacttac tatgaggatc cagacggaag ttatgaggca tttagtgtca
     2221 aaatacctaa acttgaacga gagaaagttg gggtattcta tgacacagac gtggacggtg
     2281 actatctaaa aaaaattcat ttcgacgcgt atgagcagac catccagcgc caatttgcca
     2341 acgaagtaaa aacgacacag cctgttccca gtccgatgat tcaggctctg accgttcata
     2401 acaagactga cacccatgat aagcaataca ctcaggcggt cacattgcag agtcactttt
     2461 cgctgcctgc taataatgaa cttgttaccg gtgcacagta caaacaagac agggtcagcc
     2521 aaaggtccgg tggcatgacc tcaagcaaat ctctgaccgg cttcattaat aaggaaacac
     2581 gaactcgctc ctattatgag tcagagcaaa gtacagtctc actattcgca caaaatgact
     2641 ggcaattcgc cgatcactgg acatggacaa tgggagttcg ccaatactgg ctttcttcaa
     2701 agttgacgcg tggtgacgga gtatcatata ccgcaggcat tataagcgat acctctcttg
     2761 ccagagagtc tgcgagtgat cacgaaatgg taacatctac aagcctgcgc tattcaggtt
     2821 tcgataacct ggagttacgc gctgcgttcg cgcaaggcta cgtatttccc acactctccc
     2881 agctttttat gcagacatct gcgggcggca gtgtcacata cggaaatcct gatcttaagg
     2941 ctgaacactc caataacttt gaattaggtg cacgatataa tggtaatcag tggctgattg
     3001 acagcgcagt ttactactca gaagctaaag attatattgc aagtctgatc tgtgatggca
     3061 gtatagtttg caatggtaac accaactcct cccgtagtag ctactattat tatgacaata
     3121 ttgatcgggc aaaaacatgg ggactggaaa taagcgcgga atataatggc tgggttttct
     3181 cgccatatat cagtggcaat ttaattcgtc ggcaatatga aacttcaaca ttaaaaacaa
     3241 ctaatacagg tgaaccagcg ataaacggac gtatagggct gaaacatact cttgtgatgg
     3301 gtcaggccaa cataatctct gatgttttta ttcgtgctgc ctctagtgca aaagatgaca
     3361 gtaacggtac cgaaacaaat gttccgggct gggccactct caactttgca gtaaatacag
     3421 aattcggtaa cgaggatcag taccggattt acctggcact caataacctg acagacaaac
     3481 gctaccgtac agcacatgaa actattcctg cagcaggttt taatgcagct ataggttttg
     3541 tatggaattt ctgatgacaa aaacaatcgt tatggaattc tttgtcctga tctgcccgct
     3601 ttgcgggcag gctaacgaac gtattgtgtt tgttgaaggt ttttcgacgt tgttttccat
     3661 ctgtgggtga atatttattc ttcccgccag catgactcta tactcattgc tacgacaatg
     3721 acttataaag ctggttactg aacctatacc tttgtaattc aaaagtgata tcagttcaca
     3781 acgcaatact atttttagat tatttactct cacccagata ctgaatcaat ggataatacc
     3841 ttttgattta tcccgacgcg gaagaaggtg gtgaaccgcc ccggaaatcc tggagactaa
     3901 actccctgag aaagaggtaa acaggatgac taaaaatac
//
LOCUS       DAZKIP010000234         3933 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZKIP010000234 DAZKIP010000000
VERSION     DAZKIP010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3933
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.236"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..1529)
                     /locus_tag="ACHOUK_004735"
     CDS             complement(<1..1529)
                     /locus_tag="ACHOUK_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HFP9900004.1"
                     /translation="MNRNCYRIIFNKARGMLMVVADIARSGRAGTSLSSRTGYPHRQR
                     ICRVTPLAFSLWLASGMVHSVSAAGIVADHGAPGHQQPTITQTASGIPQVNIQTPSAG
                     GVSHNTYSQFDVGNQGVILNNAHNNVQTQLGGMVAGNPWLARGEARIILNEVNSRNPS
                     QLNGFVEVAGKKAQVVIANPAGISCDGCGFINANRATLTTGQPQMKNGSLTGFSVERG
                     EIQITGKGMDASRTDYTDIIARSVKINAGIWAQDLKVTTGRNNVDIAHGQTEKKAADA
                     SSQPQVALDVSSLGGMYAGKIRLVGTETGVGVRNAGHIGAQAGEVTLTADGRIENSGS
                     ISAKTDVHLATTRELHNSGSVYAGQDTQIQSDGVFTHTGSVASRRNTRIQTARLIGGE
                     RSLLAAGVKDDGRLAAAGNLTVSTTGELAAHGQVLSGGNMQLKGQGLDLSNSRIQGQH
                     TELNATSGNLSTQNAQLSAGTLSARTAGQFSNNGGTINADTLQISAQSLSNRKGSLIQ
                     TG"
     gene            complement(1545..2072)
                     /locus_tag="ACHOUK_004736"
     CDS             complement(1545..2072)
                     /locus_tag="ACHOUK_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HFP9900005.1"
                     /translation="MRNGKYDVLSPLYSGEPVNEAEVLGAAVWLWMHSPLHRDAPLHT
                     LPDLLLPVIKHRQYVVATEQGRPVFFMSQAWLSPEAEARYLTQPAILMPQSDWNSGDR
                     MWVCDWVAPFGHTPDMSRLVRQDIFPRLCWRSLYHRGQQTGLRVMNFRGAQVSREEAR
                     QWRVQHPLAVDVPER"
     gene            complement(2082..3161)
                     /locus_tag="ACHOUK_004737"
     CDS             complement(2082..3161)
                     /locus_tag="ACHOUK_004737"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFP9900006.1"
                     /translation="MEIVPGEQPGESDILITRKQDKYWRLGFSFDDSGTRSTGRYLGG
                     ITFSLDNPFSLSDLLYVSATHNFPHYGGKGSKNYTAHYSVPFGYWQFSVTASDYDYHQ
                     TVAGAVEDYQYSGESQNLGMQLSRVLHRNGTQKTVLTYDVQARRSRNYINDTEIQVQR
                     RQTAAWRLGLQHRHYISQAILDAGVSWQRGTRWFGAQPAPEEIFGEATALSKILQLNA
                     SFNLPFTLAGQPFSYSFRYQRQLSNTPLTPQEQFAIGNRWTVRGFDGERTLNASNGWY
                     IRNDLAWRTPLPEQALYLGMDYGEVGGAGSEYLVGRHLAGGVVGLRGQLFSTDYDVFI
                     GRPFSKPAGFMTSDVTAGFNLFRGF"
     gene            complement(3159..3734)
                     /locus_tag="ACHOUK_004738"
                     /pseudo
     CDS             complement(3159..3734)
                     /locus_tag="ACHOUK_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
BASE COUNT          902 a         1197 c          960 g          874 t
ORIGIN      
        1 cccgtctgaa tcagactgcc tttacgattt gacaggcttt gtgccgatat ctgcagtgtg
       61 tcggcattta tcgtaccgcc attattgctg aactgcccgg cagtccgggc ggaaagcgta
      121 ccggcactca gttgcgcatt ctgtgtagac agatttccac tggttgcatt cagctcagta
      181 tgttgtccct gtatccggct gttgcttaaa tccagcccct gtcccttcag ttgcatgttc
      241 ccaccggaca gaacctgtcc gtgtgccgcc agttcgccgg tggtactgac agtcaggttg
      301 cccgctgccg ccagacgtcc gtcatctttc accccggcgg ccagcagact ccgctcaccg
      361 ccgatcagac gcgctgtctg aatccgggtg ttgcgacgtg atgccacaga gcctgtatgg
      421 gtgaatacac catcactctg tatctgcgta tcctgccccg cataaacaga accactgtta
      481 tgcagctccc gggttgtcgc cagatgcaca tccgttttcg cactgatact gccactgttt
      541 tcaattcgtc cgtcagccgt cagggtaacc tcccctgcct gtgcaccaat atgaccggcg
      601 ttacgaacac ccacccccgt ttccgtaccg acaaggcgaa tttttccggc atacattcca
      661 ccaaggctgg acacatccag cgccacctgt ggctgcgaag aggcatcagc ggcttttttc
      721 tcagtctgcc cgtgtgcaat atccacattg ttacgtccgg tggtcacttt caggtcctgc
      781 gcccagatac cggcattaat cttcacagac cgggcaatga tatcggtata atccgtgcgc
      841 gaggcatcca tccctttgcc cgtaatctga atctcaccac gttcaacact gaatccggtc
      901 agactgccgt ttttcatctg cggctgcccc gtggtcagcg tggcccggtt cgcattaata
      961 aaaccgcacc cgtcgcaact gatgccggcc gggttcgcaa tcaccacctg cgcctttttc
     1021 ccggcaacct caacaaaacc gttcaactgg ctgggattgc gactgttcac ttcattaaga
     1081 atgattctgg cctctcccct ggccagccac gggttcccgg ccaccatgcc acccaactga
     1141 gtctgcacat tgttatgggc attattcaga atgacgccct ggttaccgac gtcgaactgg
     1201 ctgtatgtat tatgtgaaac cccgccggca gaaggcgtct gaatattcac ctgcggaatg
     1261 ccgctggccg tctgcgtaat cgtgggctgc tgatgccccg gtgcgccatg gtctgcgaca
     1321 atgcctgcag cactgacaga atgcaccatc cccgacgcca gccacaaaga gaatgcaagc
     1381 ggcgtgacac ggcagatacg ttgcctgtgt ggatatcctg tgcgggagga cagtgatgta
     1441 ccggcgcgac cggaacgggc aatatctgcc accaccatca gcattccccg ggctttgtta
     1501 aaaataatgc ggtaacaatt tctgttcatt ctgataaacc tgacttatct ctccggcaca
     1561 tccacagcaa gcgggtgctg cacacgccac tggcgcgcct cttcacgact gacctgtgca
     1621 ccccggaaat tcatcacccg caacccggtc tgctgcccac ggtggtacag cgaacgccag
     1681 cacagacggg gaaaaatatc ctgccggaca agacggctca tatccggagt gtgtccaaaa
     1741 ggggcaaccc agtcacagac ccacatccgg tcgccgctgt tccagtccga ctgaggcatg
     1801 agaattgccg gctgcgtaag ataacgcgcc tctgcctccg ggctgagcca ggcctggctc
     1861 atgaaaaaaa ccggtcgtcc ctgctctgtt gccaccacat actgtcggtg cttaatcacc
     1921 ggcagcagca ggtccggcag ggtatgcaat ggcgcatcac gatgcagggg ggagtgcatc
     1981 cacagccaga cggccgcccc cagaacttcc gcctcattga ccggctcacc ggaatacagg
     2041 ggactcagaa cgtcatattt cccgttacgc atgaaatttt ctcagaaacc ccggaacaga
     2101 ttaaaaccgg ctgtcacatc actcgtcata aaacccgccg gttttgaaaa cggacgaccg
     2161 atgaaaacgt cataatccgt actgaacagt tgccccctca gccccacaac accaccggcc
     2221 agatgacgtc ccaccaggta ttcactgccg gctcccccca cctcgccgta atccattccc
     2281 agatataacg cctgctccgg cagtggcgta cgccaggcca ggtcgttgcg gatataccag
     2341 ccgttactgg cattcagcgt tcgctccccg tcaaaaccac gaaccgtcca gcggttaccg
     2401 atggcaaact gttcctgtgg agtcagcggg gtattgctca gctggcgctg ataccggaag
     2461 gaataactga agggctgccc tgcaagggta aagggcagat taaaggatgc attcaactgc
     2521 aggatcttac tgagcgccgt tgcctcaccg aagatttcct ccggtgccgg ctgtgcacca
     2581 aaccagcggg ttccccgttg ccagctgacg ccggcatcca gtatggcctg cgaaatataa
     2641 tgacggtgct gcagtccaag tcgccatgcc gccgtctgcc ggcgctggac ctggatttcc
     2701 gtatcattaa tgtagttacg cgacctgcgg gcctgaacat cgtaagtcag caccgttttc
     2761 tgcgtcccgt tacggtgcag tacccggctc agctgcatgc ccagattctg actctcgcca
     2821 ctgtactggt aatcctcaac cgcaccagct accgtctgat ggtaatcgta atcactggcg
     2881 gtgacggaaa actgccagta gccgaaaggc actgagtaat gggccgtgta gtttttactg
     2941 cccttacccc cgtaatgtgg aaaattatgg gtggcagaaa cgtacagcag gtcgctcagg
     3001 gaaaaaggat tatccagaga aaatgttata ccaccgagat aacgccctgt gctgcgggta
     3061 cctgaatcgt caaacgaaaa ccccagtcgc cagtatttgt cctgtttgcg ggtaataaga
     3121 atgtcacttt cccctggctg ctcaccgggt acaatttcca tatcagcctg cacacccggc
     3181 agtcgttgca tattctccag cccctgttca atgtcccgta aatccagcag agagccttcg
     3241 tgtgccggaa atgaggaata caactgaata tagtcatcac tgcccggtgt cagacgcaca
     3301 tgtcgtacaa caccgggaat aataaccagc cggaggatac cgctttttaa atcctgcgaa
     3361 ggtgccagaa cccgggtggt gacataacca tgatcaacca gacggttctg caatgtactc
     3421 atcagcagat taattccttt cgcccccagg caatgcccga ccgccccgtt ggcgattttt
     3481 tgtaaaggca gccagtgtgg taacgcatca gcccccttca gttccgtctg tttaatctga
     3541 aaacagggcg tttcaacagg aaaattaatc ttatgggcaa aagagccagg tgcagaaaga
     3601 cgaacatcag gggctgaagg cgtcagttgt tgctcaaggg cttcctgacg ctgctgctga
     3661 atgataaatg tatcctctcc actacgccgg atatccgcgg cctgaagaga taaaggcgat
     3721 aataaaacag acatcagtgt cagtgacagc aaggacggac agcgccggaa taagctttgt
     3781 tcctcagccc tctcgttcct gcaacataat tttccagtca aacgaaaaac cttttattca
     3841 tgttatacca acaatcacca gcctcctgac gatacctccc accatgtttt gcgatggcac
     3901 gctaccgccc ttccggtatt tacccgcatc aac
//
LOCUS       DAZKIP010000235         3885 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZKIP010000235 DAZKIP010000000
VERSION     DAZKIP010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.237"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..792
                     /locus_tag="ACHOUK_004739"
     CDS             <1..792
                     /locus_tag="ACHOUK_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFP9900007.1"
                     /translation="HLRKLTSHEPVPGIMGKSGAGKSSLCNALFQGEVTPVSDVHAGT
                     REVQRFRLNGHGHSMVITDLPGVGESRDRDAEYETLYRDILPELDLVLWLIKADDRAL
                     SVDEYFWRHILHRGHQQVLFVVTQADKTEPCHEWDMAGIQPSPAQAQNIREKTDAVFR
                     LFRPVHPVVAVSARTGWELDTLVSALMTALPDHAASPLMTRLQDELCTESVRGQAREQ
                     FTGAVDRIFDTVESVCIASVARTVLRAVRDTVVSVARAVWNWIFF"
     gene            1165..>3885
                     /gene="cah"
                     /locus_tag="ACHOUK_004740"
     CDS             1165..>3885
                     /gene="cah"
                     /locus_tag="ACHOUK_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding autotransporter Cah"
                     /protein_id="HFP9900008.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRTGVAVALSLAA
                     VTSVPALAADTVVQAGETVNGGTLANHDNQIVFGSTNGMTISTGLELGPDSEENTGGQ
                     WIQNGGIAGNTTVTTNGRQVVLEGGTASDTVIRDGGGQSLNGLAVNTTLNNRGEQWVH
                     EGGVATGTIINRDGYQSVKSGGLATGTIINTGAEGGPDSENVSSGQMVGGTAESTTIN
                     KNGRQVIWSSGVARDTLIYAGGDQTVHGHALNTTLNGGYQYVHKDGLALNTVINEGGW
                     QVVKAGGAVGNTTINQNGELRVHAGGEATAVTQNTGGALVTSTAATVTGTNRLGHFSV
                     GNGMADNVVLENGGRLDVLEGHSAQNTLVDDGGTLAVSAGGKATDVTMTSGGALIADS
                     GATVEGTNASGKFSIDGISGQASGLLLENGGSFTVNAGGQAGNTTVGHRGTLTLAAGG
                     SLSGRTQLSKGASMVLNGDVVSTGDIVNAGEIHFDNQTTQDAVLSRAVAKGDSPVTFH
                     KLTTTNLTGQGGTINMRVRLDGSNTSDQLVINGGQATGKTWLAFTNVGNSNLGVATTG
                     QGIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYA
                     SMLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGS
                     YGFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLN
                     LVHTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQ
                     LQYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSA
                     KHSVRELPVNWWVQPSAIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDL"
BASE COUNT          891 a         1027 c         1176 g          791 t
ORIGIN      
        1 catctgcgca aactcaccag tcacgaaccc gtgcctggca ttatgggtaa aagcggggcc
       61 ggtaaatcct cactctgtaa tgcgctgttt cagggggagg tcaccccggt cagtgatgtt
      121 cacgccggca cccgggaagt gcagcgcttc cgtctgaatg gccatggtca cagcatggtt
      181 atcactgacc tgcccggggt gggcgagagc cgggacaggg atgcagagta tgagaccctg
      241 taccgtgaca ttctgcctga actggacctg gtgctgtggc tgattaaagc cgatgaccgg
      301 gccctgtctg tggatgagta tttctggcga cacatcctgc accggggaca tcagcaggtg
      361 ctgtttgtgg tgacgcaggc cgacaaaacg gagccctgcc atgaatggga tatggccggc
      421 attcagcctt ctcccgcaca ggcacagaac attcgcgaaa aaacagatgc ggtattccgt
      481 ctgttccggc ccgtacatcc ggttgttgcc gtatcggccc gcaccggctg ggagctggat
      541 acgctggtca gtgcgctcat gacagcgctt cccgaccatg ccgccagtcc cctgatgacc
      601 cgcctgcagg acgagctgtg cacggaatct gtccgggggc aggcccgtga acagtttacc
      661 ggtgcggtgg accggatatt tgacacggtg gagagcgtct gtattgcctc tgttgcacgc
      721 acggttctgc gtgccgtccg tgacacggtg gtctctgttg cccgcgctgt atggaactgg
      781 attttcttct gaacagtcta ccaggggaaa tgcctgatta cattgcgtta accaactgaa
      841 aacatgatac aaacacctca tgcccctcct gtttctcttg cgtgactgct ctgctgttaa
      901 tagaataaaa cgatcgataa aacaggtatc gatcgttact atcgatcgtt tatagtggtt
      961 aatggcctgc gaaaaggcag ctgaatctct tcatcatgca gaacggaatt gcacacaacg
     1021 gactgatact tctctggcgg atgagagagg ggaagcattt atgtccggga aaaaccactt
     1081 tatctgacca tgctgttttg tacctgccgg tatccacatt tgtgggtacc ggctttttta
     1141 ttcaccctct gtaaggaaaa gctgatgaaa cgacatctga acaccagcta caggctggta
     1201 tggaatcaca ttacgggcac cctggtggtg gcctccgaac tggcccgctc acggggaaaa
     1261 cgcaccggtg tggcggttgc actgtctctt gcggctgtca cgtcagttcc ggcactggct
     1321 gctgacacgg ttgtacaggc aggagaaacc gtgaacggcg gaacactggc aaatcacgac
     1381 aaccagattg tcttcggttc gaccaacgga atgaccatca gtaccgggct ggaactgggg
     1441 ccggacagtg aagaaaacac cggtgggcaa tggatacaga atggcgggat agccggaaat
     1501 accactgtca ccacaaatgg tcgtcaggtc gtgctggagg ggggaacagc cagtgatacg
     1561 gttattcgtg acggcggggg acagagcctg aacgggctgg cggtgaacac cacgctgaat
     1621 aacagaggcg agcagtgggt tcatgagggc ggggttgcca ccggtacaat tatcaaccgc
     1681 gacggttacc agagcgttaa aagtggcggg ctggcaacag gaaccatcat caacaccggc
     1741 gcagaaggcg gcccggattc tgaaaatgta tccagcggtc agatggtcgg gggaacagca
     1801 gaatccacca ccatcaacaa aaatgggcgg caggttatct ggtcttcagg ggtggcccgg
     1861 gataccctca tttacgcagg tggtgaccag acggtacacg ggcatgcgct gaataccaca
     1921 ctgaatggcg gttaccagta tgtgcacaag gacggacttg cgctgaacac ggtaattaac
     1981 gaggggggct ggcaggttgt taaggcaggt ggtgctgtcg gtaacaccac cataaatcag
     2041 aacggtgaac tgagggtaca tgccggcggg gaagccactg cagtcaccca gaacacgggc
     2101 ggtgcactgg ttaccagtac tgctgcaact gtcaccggca caaaccgcct ggggcatttc
     2161 tccgtgggaa atggcatggc agataacgtc gttctggaaa atggcggtcg cctggatgta
     2221 ctggaaggcc attcagcgca gaataccctg gtggatgacg gcggtaccct ggcagtgtct
     2281 gccggtggta aggcaacaga tgtcaccatg acatccggtg gtgccctgat agcagacagt
     2341 ggtgccactg ttgaggggac caatgccagc ggtaagttca gtattgatgg catatccggt
     2401 caggccagcg gcctgctact ggaaaatggc ggcagcttta cggttaatgc cgggggacag
     2461 gcaggcaaca ccactgtcgg acatcgtgga acactgacgc tggctgccgg gggaagtctg
     2521 agtggcagaa cacagctcag taaaggcgcc agtatggtac tgaatggtga tgtggtcagt
     2581 accggcgata ttgttaacgc aggagagatt cactttgata atcagacgac acaggatgcc
     2641 gtgctgagcc gtgctgttgc aaaaggcgac tccccggtaa cgttccataa actgaccacc
     2701 accaacctca ctggtcaggg cggcaccatc aatatgcgtg ttcgccttga tggcagtaat
     2761 acctcagacc agctggtgat taatggtggt caggcaaccg gcaaaacctg gcttgcgttc
     2821 acaaatgtcg gaaacagtaa cctcggggtg gcaaccaccg gacagggtat ccgggttgtg
     2881 gatgcacaga atggtgccac cacagaagaa ggtgcgtttg ccctgagccg cccgcttcag
     2941 gccggcgcct ttaactacac cctgaaccgt gacagcgatg aagactggta cctgcgcagt
     3001 gaaaatgctt atcgtgctga agtccccctg tatgcctcca tgctgacaca ggcaatggac
     3061 tatgaccgga ttctggctgg ctcccgcagc catcagaccg gtgtaaacgg tgaaaataac
     3121 agcgtccgtc tcagcattca gggcggtcat ctcgggcacg ataacaacgg cggtattgcc
     3181 cgtggggcca cgccggaaag cagcggcagc tatggcttcg tccgtctgga gggtgacctg
     3241 ctcagaacag aggttgccgg tatgtctctg acgacagggg tgtatggtgc tgcaggccat
     3301 tcttccgttg atgttaagga tgatgacggc tcccgcgccg gcacggtccg ggatgatgcc
     3361 ggcagtctgg gcggatacct gaatctggta cacacatcct ccggcctgtg ggctgacatt
     3421 gtggcacagg gaacccgcca cagcatgaaa gcatcatcgg acaataacga cttccgcgcc
     3481 cggggctggg gctggctggg ctcactggaa accggtctgc ccttcagtat cactgacaat
     3541 ctgatgctgg agccacaact gcagtacacc tggcagggac tctccctgga tgacggccag
     3601 gataacgccg gttatgtgaa gttcgggcat ggcagtgcac aacatgtgcg tgccggtttc
     3661 cgtctgggca gccacaacga tatgaccttt ggtgaaggca cctcatcccg tgacaccctg
     3721 cgcgacagtg caaaacacag tgtgcgtgaa ctgccggtga actggtgggt acagccttct
     3781 gctatccgca ccttcagctc ccggggtgac atgagcatgg ggacagccgc agccggcagt
     3841 aacatgacgt tctcaccgtc ccggaatggc acgtcactgg acctg
//
LOCUS       DAZKIP010000236         3870 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZKIP010000236 DAZKIP010000000
VERSION     DAZKIP010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.238"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..1435
                     /locus_tag="ACHOUK_004741"
     CDS             <1..1435
                     /locus_tag="ACHOUK_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFP9900009.1"
                     /translation="FKILLGRLTSWSMLYLKQHLRRNVSTRSSFSAETYRDEFDRFIK
                     RFPIIGSSTHSIINSIGKGALLDYVIIDEASQQDIVPGILGLGCARNVIVVGDRKQLP
                     HVPVLLPNSPSPPAEYYNCEKYSLLDSVCMLFRNMVPVTLLKEHYRCHPKIIQFCNKQ
                     FYDNALIPLTVDSGEASLSLVITAKGNHTRNFSNLRELESLEGHYWDEESSRGYIAPY
                     NAQVNLAEKVLPADFVKSTVHKFQGRECDEIVFSTVLDKKRSSQHSRNIAFVDNPELV
                     NVAVSRARNKFTLVTGNDVFERHAGHIAALIRYIKYYADDGEIFESPVISAFDLLYSE
                     YDKSLERLNSRLNSNDSHFKSEQIVACLLRDILSQDSYRSMMFHSQIALNQLVLLERG
                     DFTHREQLFMRNRASCDFVVYYKVGKTPLGVIEVDGGYHLTSVQAERDELKNSILKKC
                     GLPLLRLRTIDSDIEGKLGAFLSGLTG"
     gene            1936..3168
                     /locus_tag="ACHOUK_004742"
     CDS             1936..3168
                     /locus_tag="ACHOUK_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFP9900010.1"
                     /translation="MSDTLLTEKILTGENVLRAAIARIEWIFETFPSVCLSFSGGKDS
                     TVLFHLVAEVARRRKRHFSVLFIDWEAQYRCTIEHIQKMREMYHDVTETFYWVALPLT
                     TVNGVSQFQPEWICWEPGVTWVRQPPEEAITDMTYFPFFRYAMTFEEFVPAFSSWFAG
                     NRCGVAVLTGVRADESLNRFMGLVSQRKLRYADDKPWTTASPEGFYYTMYPLYDWKAR
                     DIWIYNARACAIYNPLYDLMYRADVPLRNMRVCEPFGPEQRKGLWLYHVLEPETWARM
                     CERVSGAASGALYANESGAYFALRKRISKPAHHTWRSYAMFLLDVMPERTAEHYRNKI
                     AVYLRWYQTRGFPDDIPDEQENDLGSRDIPSWRRICKTLIKNDFWCRPLSFSPNKPRH
                     YERYLQRMKERRKEWGIL"
     gene            3153..3791
                     /locus_tag="ACHOUK_004743"
     CDS             3153..3791
                     /locus_tag="ACHOUK_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HFP9900011.1"
                     /translation="MGDSVTPEIIGNMIRQYFSQERPEEETIQALNHLRRVLHEVSPF
                     AHEPVDCVLWVKADEVVANDYNPNVMAPGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQVLGREAGTGKRLKGWLPVVCINPERKGQASRIAATIRHNRARGKHQITSMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSPAWTVR"
BASE COUNT         1029 a          776 c         1009 g         1056 t
ORIGIN      
        1 ttttaaaatt ttactggggc gcctgacatc atggtctatg ctttatttaa agcaacatct
       61 gcgaagaaat gtttcaacaa gaagttcatt ctcggcagag acatacaggg atgaatttga
      121 ccgttttata aagcgttttc cgattattgg tagcagtacg cattcgatta ttaactctat
      181 cggcaagggg gcccttcttg attatgttat tattgatgaa gcctcgcagc aggatattgt
      241 tccgggcatt ctgggactgg gatgtgcccg taatgtaatc gttgtagggg atcgtaagca
      301 acttccacat gttcctgtat tactcccgaa cagcccatct ccaccggcag aatactataa
      361 ttgtgagaaa tatagtctac ttgattcggt ttgtatgttg ttcaggaaca tggtgccagt
      421 aacccttctt aaagagcatt atcgttgtca tcctaaaatc attcaatttt gtaataagca
      481 gttttatgat aatgctctga taccgttaac ggttgattcc ggtgaagcct cattatctct
      541 ggtcatcacg gcaaaaggaa accatacccg taatttttca aatctcaggg agcttgaatc
      601 tctggaaggt cattactggg atgaagaaag tagcagggga tacattgccc catataatgc
      661 acaggttaat ctggctgaaa aagtgctgcc tgctgatttt gtgaaatcga cggtgcataa
      721 atttcaggga cgggagtgcg atgaaattgt gttttccact gtgctggata agaagcgcag
      781 cagccagcac agtcggaaca tcgcgtttgt tgataatcct gaactggtga atgtcgctgt
      841 ttcaagggcc cgaaataaat ttacgctggt gactggtaat gatgtatttg aacgccacgc
      901 tggacatatt gcggcgctga ttcgttacat caaatactac gctgatgacg gagagatttt
      961 cgagagcccg gtcatatcag catttgattt gctgtacagt gagtatgaca aatcactgga
     1021 acgactgaat agtagattga attcaaatga ttcacacttt aagtcagaac agattgtggc
     1081 ctgcctgttg cgggatatcc tgtcgcagga tagttatcgg agtatgatgt ttcattcaca
     1141 aatagcgctg aatcagttgg tgttgctgga gcggggggat tttacacacc gtgaacagtt
     1201 attcatgcgt aaccgggcca gttgtgactt tgttgtttac tataaggtcg ggaagactcc
     1261 attaggggtt attgaagtgg atggaggtta tcatctgacc agtgttcagg ctgaacgtga
     1321 tgaacttaaa aacagtatac tgaagaaatg cggcctgcca ttgctaagat tacgtactat
     1381 agacagtgat atagaaggga agttgggagc gtttctgtca ggcctgacag gctaaaatct
     1441 gaataaaaaa tattgcagcg ggaggtctca aacctgattt ggtcatgtgc cacacacaat
     1501 tgtacgaacc ccatggacac aggcttcttt gttttggtta catcaacgta cgtattcctg
     1561 atacatataa aaataagtgc tattgctcaa agctaacagg gaatgtttct gttgaataat
     1621 tccttagtga ataaacttta tcagggggta attctattac agtgaatact tcctgcaaaa
     1681 tgatatttga attgttcacc atctgcatca tattttctta tttacaagtt gaaaggatag
     1741 tatatgttga aaatgcatgg attttccagg tgtgttacca gtaagaagca ggtggataaa
     1801 ggatgtgata acaaatccaa caaccaggtt aatatctgaa gatttttgtt gttattattt
     1861 atccccaaag cggtgagtta ttaatgaagt ttgcctgaat gtataaacag aattattcga
     1921 cataaagcca ggaaaatgag tgatacgctt ttaaccgaaa aaatccttac cggagaaaat
     1981 gttttgcgtg ctgcaatcgc ccgcattgag tggatttttg aaacatttcc gtcggtatgt
     2041 ctgtcttttt caggtggaaa ggattccacc gtgcttttcc atcttgtggc agaggtggcc
     2101 cgcaggagaa aacgtcattt ctctgttctg ttcattgact gggaggccca gtatcggtgc
     2161 accattgaac atattcagaa gatgcgggaa atgtaccatg atgtgacgga aaccttttac
     2221 tgggtggcac ttcccctgac cacggtaaac ggcgtctcgc agtttcagcc ggagtggata
     2281 tgctgggagc caggagtgac ctgggttcgc cagccaccgg aagaggccat tacggatatg
     2341 acatattttc cctttttccg gtatgccatg acgtttgaag aatttgttcc ggcattttct
     2401 tcctggtttg ccggtaaccg gtgtggagtg gcagtgctga ccggtgttcg tgcggatgaa
     2461 tccctcaatc gctttatggg gctggtgtct cagcgcaaac tgagatatgc cgatgataag
     2521 ccctggacca cggcatcgcc tgaagggttt tattacacca tgtatccgtt gtatgactgg
     2581 aaagcccgcg atatctggat atataacgcc agagcttgtg ccatttacaa tcctctgtat
     2641 gacctgatgt accgtgccga cgtgccgtta cgcaacatgc gtgtctgtga accttttggt
     2701 cctgaacaac gtaaggggct gtggctttac catgttctgg agccggaaac ctgggccagg
     2761 atgtgtgagc gggtgtcagg cgctgccagc ggggcgcttt atgccaatga aagcggtgcc
     2821 tattttgccc tgcgtaaacg tatatcaaaa ccagcccatc atacctggcg cagctatgcg
     2881 atgttcctgc tggatgtgat gccggaaaga acggcagaac attaccgtaa taaaattgct
     2941 gtctacctgc gctggtatca gacgcggggc ttcccggatg acatcccgga tgaacaggag
     3001 aatgacctgg ggagccggga tattccgtcc tggcggcgta tctgtaagac acttataaag
     3061 aatgatttct ggtgccggcc cctctccttc agtccgaaca aaccccggca ctatgaacgt
     3121 tatctgcagc gtatgaaaga aaggaggaag gaatggggga ttctgtgaca ccggagataa
     3181 ttggcaacat gattcgtcag tacttcagcc aggaacgacc agaagaagag accatacagg
     3241 cccttaatca cctgcgcaga gtgcttcatg aagtcagtcc ttttgcacat gagccagtgg
     3301 actgtgtgct gtgggtaaaa gcagatgagg tggtggccaa tgattacaat cctaatgtca
     3361 tggcgccggg tgagaagaaa ctgctgaagc agtcactgga gaaagatggt ttcacacaac
     3421 cggtggtggt gtctgaggaa aagaaccatt acctggtggt ggatggtttt catcgccagg
     3481 tgctgggcag ggaagcaggt acgggaaaac gtctgaaggg ctggctgccg gtggtctgta
     3541 ttaatccgga gagaaaggga caggcctcac gtattgctgc gaccatccga cataaccggg
     3601 ccaggggcaa acatcagata acctcaatgt ctgatattgt cagggacctg tcgcgcctgg
     3661 gctggacgaa tgaacgcatt ggtacggagc tgggtatgga ccaggatgaa gttctcagat
     3721 tgaaacagat tagcgggctg acggagctgt ttcaggaaga ggatttcagc ccggcatgga
     3781 cggttcgctg agaccgtcag atatgtatat tatttcagtg agttaatgtt ttgagcagga
     3841 aaaagagtac cccggaagtt gtggaagaca
//
LOCUS       DAZKIP010000237         3867 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZKIP010000237 DAZKIP010000000
VERSION     DAZKIP010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.239"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            6..149
                     /locus_tag="ACHOUK_004744"
     CDS             6..149
                     /locus_tag="ACHOUK_004744"
                     /inference="COORDINATES: protein motif:HMM:NF019939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900012.1"
                     /translation="MTSVANDINKIISSVSGFDSGDERLASLYNLSLHQNNTHSTTLT
                     TTV"
     gene            199..2319
                     /gene="ehxB"
                     /locus_tag="ACHOUK_004745"
                     /gene_synonym="hlyB"
     CDS             199..2319
                     /gene="ehxB"
                     /locus_tag="ACHOUK_004745"
                     /gene_synonym="hlyB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter
                     permease/ATPase EhxB"
                     /protein_id="HFP9900013.1"
                     /translation="MMSKCSSHNSLYALILLAQYHNITVNAETIRHQYNTHTQDFGVT
                     EWLLAAKSIGLKAKYVEKHFSRLSIISLPALIWRDDGKHYILSRITKDSSRYLVYDPE
                     QHQSLTFSRDEFEKLYQGKVILVTSRATVVGELAKFDFSWFIPSVVKYRRILLEVLTV
                     SAFIQFLALITPLFFQVVMDKVLVHRGFSTLNIITIAFIIVILFEVILTGARTYIFSH
                     TTSRIDVELGAKLFRHLLALPVSYFENRRVGETVARVRELEQIRNFLTGQALTSVLDL
                     FFSVIFFCVMWYYSPQLTLVILLSLPCYVIWSLFISPLLRRRLDDKFLRNAENQAFLV
                     ETVTAINTIKSMAVSPQMIATWDKQLAGYVASSFRVNLVAMTGQQGIQLIQKSVMVIS
                     LWMGAHLVISGEISIGQLIAFNMLAGQVIAPVIRLAHLWQDFQQVGISVERLGDVLNT
                     PVEKKSGRNILPEIQGDIEFKNVRFRYSSDGNVILNNINLYISKGDVIGIVGRSGSGK
                     STLTKLLQRFYIPETGQILIDGHDLSLADPEWLRRQIGVVLQENILLNRSIIDNITLA
                     SPAVSMEQAIEAARLAGAHDFIRELKEGYNTIVGEQGVGLSGGQRQRIAIARALVTNP
                     RILIFDEATSALDYESENIIMKNMSRICKNRTVIIIAHRLSTVKNANRIIVMDNGFIS
                     EDGTHKELISKKDSLYAYLYQLQA"
     gene            2323..3762
                     /gene="ehxD"
                     /locus_tag="ACHOUK_004746"
     CDS             2323..3762
                     /gene="ehxD"
                     /locus_tag="ACHOUK_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter subunit
                     EhxD"
                     /protein_id="HFP9900014.1"
                     /translation="MRFYMKGLWDLVCRYKTVFSDVWKIRHTLDAPVREKDEYAFLPA
                     HLELIETPVSRRSHFVVWSILLFVIISLLLSVLGKVEVVSVANGKFTHSGRSKEIKPI
                     ENAIVEKIMVKDGSFVKKNDPLVELTVPGVESDILKSEASLLYEKTEQYRYAILSESI
                     QRNELPEIRITDFPGGEDNAGGEHFQRVSSLIKEQFMTWQNRKNQKQLTLNKKIVERD
                     AALARVSLYEHQVSQEGRKLNDFKYLLNKKAVSQHSVMEQENSYIQAKNEHAVWLAQV
                     SQLEKEIELVREELALETNIFRSEIIEKHRKSTDNIVLLEHELEKNRQRKASSFIKAP
                     VSGTVQELNIHTEGGVVTTAETLMIIVPDNDILEVTASVLNKDIGFIQPGQEVVIKVD
                     AYPYTRHGYLTGKVKNITADSVSVPDTGLVFNVIISVDRNDIQGERKKIPVTAGMTVM
                     AEIKTGVRSVISYLLSPLKETINESLRER"
BASE COUNT         1240 a          604 c          808 g         1215 t
ORIGIN      
        1 ttaatatcac cagtgttgcc aatgatatta ataagattat ttcttcagtt tcagggttcg
       61 attcaggtga tgaacgatta gcatctttat ataatttatc cttacatcaa aacaacacac
      121 actcaacaac tttaacgaca actgtctgag actaaaagag gaggtgatgg ccattccagg
      181 aggaatggtc atcgttatat gatgagtaaa tgtagttctc ataatagtct gtatgcactg
      241 atattgcttg cacaatatca taatataact gtcaatgctg aaactataag gcatcagtat
      301 aatacccaca cacaagattt tggggtgact gaatggttac tggcagcgaa atctattggc
      361 ttaaaagcaa aatatgtaga aaaacatttt tccagattgt caataatttc tttacctgcg
      421 ttgatatggc gggatgacgg taagcattat atattgtctc gtattactaa agattcatca
      481 cgctatcttg tttatgatcc agaacaacat cagtcactaa cttttagtcg ggatgagttt
      541 gaaaaactgt atcagggaaa agtcattctg gttacgtcaa gagcaacagt agtcggagag
      601 ttagctaaat ttgatttttc ttggtttatc ccctctgttg tgaaatacag gaggatttta
      661 cttgaggtgt taactgtttc tgcttttatt cagtttcttg cgttaataac acctcttttt
      721 tttcaggttg taatggataa ggttttagtt caccgggggt tttcaacgtt aaatattatc
      781 acaatagcat ttattatagt gatacttttt gaagtgatat taaccggagc cagaacttat
      841 attttctctc atactacaag tcgtattgac gtcgaactgg gtgctaagtt attcagacat
      901 ttgcttgcat tgcctgtttc atattttgaa aatcgcaggg tcggagagac cgttgccaga
      961 gtaagggaac tggagcaaat tcgtaatttt ttaaccggac aagcgttgac atcagttctt
     1021 gatctatttt tttctgtaat atttttttgt gtcatgtggt attacagccc tcaattaaca
     1081 ctggttatat tattgtcact accttgttat gttatatggt cattgtttat atcaccctta
     1141 ttacgtcgac gtcttgatga taagtttctc aggaatgcag aaaatcaagc ttttcttgtc
     1201 gaaacggtaa cagcaataaa tacaatcaaa tccatggcag tatcaccaca gatgattgct
     1261 acatgggata aacaactggc cgggtatgtt gcttccagtt tcagggtaaa tctggttgca
     1321 atgacagggc agcaggggat acagctgata cagaaaagtg taatggtaat tagtctatgg
     1381 atgggagcac atcttgtcat atcgggagag ataagcatcg ggcagttaat cgcgtttaat
     1441 atgcttgcag gtcaggtcat tgctcctgtt atcagactgg ctcatctttg gcaggatttt
     1501 cagcaggttg gtatttctgt tgagcgcctt ggtgatgtat taaatacacc ggtagagaaa
     1561 aagtcaggca gaaatatact gccggaaatt cagggggata tcgaatttaa aaatgtcaga
     1621 tttcggtatt cttctgacgg taatgttatt ttgaataata ttaatttata catatcaaag
     1681 ggggatgtta tcggtatagt tggccgttct ggttcaggaa aaagtacatt aacaaaactg
     1741 cttcagcgct tttatatacc agagaccgga cagattttaa ttgatgggca tgatttatca
     1801 cttgcagatc cagaatggtt acgacgccag attggtgttg tattgcagga aaatatacta
     1861 ttaaatcgta gtattatcga taatattaca ttagcttctc cggctgtatc tatggaacag
     1921 gctattgagg cagccagact tgcaggtgcc catgatttta ttagagaact aaaagaaggg
     1981 tacaatacta ttgttggaga acagggcgtt ggtctttcag gagggcagcg ccaacggatc
     2041 gcaatagccc gggctcttgt tacaaatcct cgaattctta tttttgatga agcaaccagt
     2101 gctcttgatt atgagtcgga aaatataata atgaaaaata tgtcaagaat atgtaagaac
     2161 agaaccgtaa ttattattgc acacaggttg tcaactgtaa aaaatgcaaa cagaataatt
     2221 gttatggata atggctttat ttctgaagat ggcacacata aagagcttat ctccaaaaaa
     2281 gacagtttat atgcatattt atatcagttg caggcataac gtatgcgctt ttacatgaaa
     2341 ggtttatggg atttggtttg ccgttataag acggtttttt ctgatgtctg gaaaattcgt
     2401 cacacacttg atgctcctgt gagagaaaaa gatgagtatg catttcttcc tgctcacctt
     2461 gaactcattg aaacaccagt atccagacgt tctcattttg ttgtatggag tattttatta
     2521 tttgtaatta tatctcttct cttatctgtt cttgggaaag ttgaagtggt ttcagtagca
     2581 aatggtaagt ttactcatag tggaagaagc aaagaaataa aaccgataga gaatgcgatt
     2641 gtagaaaaaa taatggtaaa agatggctct tttgtaaaaa aaaatgatcc attagttgaa
     2701 ttgacggtgc ctggtgttga atctgatatt ttaaaatcag aagcatcttt gttgtatgaa
     2761 aaaacagaac aatatcgcta cgcaattctt tctgaatcaa tacaacggaa tgagcttcct
     2821 gaaataagga taacagattt tcctggcgga gaagataatg cgggaggtga acattttcag
     2881 agggttagct cattaataaa agagcaattc atgacatggc agaacagaaa aaatcaaaag
     2941 cagctcacat taaataaaaa aatagttgaa cgggatgcag cgcttgctcg tgttagcctg
     3001 tatgagcatc aggtatcaca ggaaggaaga aaactcaatg attttaagta tttgttgaat
     3061 aaaaaagctg tttctcaaca ttcagttatg gagcaggaaa atagctatat tcaggcaaaa
     3121 aatgaacatg cagtctggct tgcacaggtt tctcaacttg aaaaagaaat agaacttgtg
     3181 cgggaagaac tggcactgga gacgaatatc ttcagaagtg aaattatcga gaagcacaga
     3241 aaatcaacag ataacattgt gttgctggag catgagcttg aaaaaaacag gcagagaaaa
     3301 gcatcatctt ttattaaagc tcctgtgagt ggtactgttc aggagttaaa tatacataca
     3361 gaaggtggtg ttgtcacaac agcagaaacg ctgatgatta ttgtccctga taatgatatt
     3421 ctcgaagtaa cagcatctgt acttaacaag gatatcggtt ttatacaacc tggacaggag
     3481 gtcgttatta aagtagatgc atatccatat acacgtcatg gttaccttac agggaaagta
     3541 aaaaatataa ctgcagattc tgtttctgtt ccggatacag ggcttgtatt taacgtgatt
     3601 atatctgttg atcggaatga tatacaggga gaaagaaaaa aaatccctgt tacagctgga
     3661 atgactgtta tggcagaaat aaaaaccggg gtgcgtagtg ttatcagcta tcttcttagt
     3721 ccattaaagg aaactattaa tgaaagttta cgtgaacgtt agtatataaa taacatattg
     3781 gctttttata tgatatagat ttctgaaata atcttgtaaa gattatctat gttatttaaa
     3841 caaactatga tacagttttt atcttta
//
LOCUS       DAZKIP010000238         3851 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZKIP010000238 DAZKIP010000000
VERSION     DAZKIP010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.240"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..510)
                     /locus_tag="ACHOUK_004747"
     CDS             complement(<1..510)
                     /locus_tag="ACHOUK_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFP9900015.1"
                     /translation="MTFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVAVTGNEGKAVAVSATRAPEYYFVVVLAGQSNGMSYGEGLPLPETY
                     DRPEPRIKQLARRSTVTPGGAACRYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKK"
     gene            754..1077
                     /locus_tag="ACHOUK_004748"
     CDS             754..1077
                     /locus_tag="ACHOUK_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFP9900016.1"
                     /translation="MNWIVIDTVIQPACGISFSAIWCNIKLILWYQSDTFLPPGCIFT
                     PTRTGVILNNKELPVTIYNVAPFNKNVWNLIKNSQECPANTGNITDKCFNHRCILEIC
                     PYGRK"
     gene            complement(1374..1643)
                     /locus_tag="ACHOUK_004749"
     CDS             complement(1374..1643)
                     /locus_tag="ACHOUK_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2c subunit B"
                     /protein_id="HFP9900017.1"
                     /translation="MKKMFMAVLFALVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
     gene            complement(1655..2614)
                     /locus_tag="ACHOUK_004750"
     CDS             complement(1655..2614)
                     /locus_tag="ACHOUK_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HFP9900018.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     DSQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            complement(2707..2783)
                     /locus_tag="ACHOUK_004751"
     tRNA            complement(2707..2783)
                     /locus_tag="ACHOUK_004751"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2747..2749),aa:Arg,seq:tct)
     gene            complement(2797..2873)
                     /locus_tag="ACHOUK_004752"
     tRNA            complement(2797..2873)
                     /locus_tag="ACHOUK_004752"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2837..2839),aa:Arg,seq:tcg)
     gene            complement(2875..2950)
                     /locus_tag="ACHOUK_004753"
     tRNA            complement(2875..2950)
                     /locus_tag="ACHOUK_004753"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2915..2917),aa:Ile,seq:cat)
     gene            complement(3264..3752)
                     /locus_tag="ACHOUK_004754"
     CDS             complement(3264..3752)
                     /locus_tag="ACHOUK_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFP9900019.1"
                     /translation="MGIRELNLTKEQHEWLNGWLELWGAWVYSGRLEKRMSSVIAKFM
                     ESVEPGRVMTRPMCNDDDGMLISQVVDSVMYIDKKAFGILLSYYAHGSSKHAIASYYH
                     SVARPRKMLCRGGGRIQKPSLATCRREVDEILNASLFMIYPILDSAFKNRKRVEKIKH
                     VA"
     gene            complement(3743..>3851)
                     /locus_tag="ACHOUK_004755"
     CDS             complement(3743..>3851)
                     /locus_tag="ACHOUK_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900020.1"
                     /translation="AVKPLKFANQMYIDTGAVFCGNLTLIQVQGEGAWA"
BASE COUNT         1071 a          976 c          802 g         1002 t
ORIGIN      
        1 ttttttggcg atatgcagcc cctgccccac ggttccgtac tgcccctttg acaggtccgc
       61 tttcggatgg ttaagacggc tcatgtcctg cacatcatgc agacaatggt ccgccggaat
      121 gatgtcgtta tatctgcatg ctgcaccacc cggtgtcacc gtactgcgac gcgccagttg
      181 cttaatacgc ggctccggac ggtcatatgt ctccggcagc ggaagacctt caccatacga
      241 catgccgttt gactgccctg ccagaaccac aacaaagtaa tactccgggg ctctggtggc
      301 actgacagcc accgccttac cctcattacc ggtcaccgcc actggtgtgg tgacatcacc
      361 ttccgccgca atggcctgca tcagggtgta aggcgtgatg gccaccggac tgccaaatgg
      421 ctgccagccc tccttcagtt tttgtgtcag tcgttcagca aggtctgacg gcgatgccgc
      481 cctgaccaca tcgtagtgtt taaatgtcat aacctctcct gccgccagca aagtaattat
      541 ccggaacaca caatcatgta acacataaaa aacacgctat aaaaaaacca tgtctcttat
      601 ataacaaaaa aacgccgaca atttaaaatg ccctcttatt ttaaacaaag aataaacgac
      661 accagaaaaa caactcagcc tgacagattg ctttcaaaac atcacctata atttatggca
      721 tcatttaaaa ccttaagaaa ataaggaaag caaatgaact ggatagtgat tgacacggta
      781 atccagccag catgtggcat atcattttca gccatatggt gtaacataaa attaatatta
      841 tggtatcagt cagatacctt cctccctccg ggctgtattt ttacaccaac ccggacaggc
      901 gtaatactca ataataaaga gctccccgtc accatttaca acgtggcccc atttaataaa
      961 aacgtctgga atttaattaa aaacagccag gagtgccctg caaacacagg aaatatcaca
     1021 gataagtgtt ttaatcatcg ctgcattctg gaaatatgcc catacgggcg aaaataacag
     1081 aatcattcag ataaaaagcc cctccggaga ggggctgaca ctgcgtatct gtatcatcat
     1141 gtacatggtg ccgggtgcct cccggtgagt tcagtccggt gtccctgaac ccgcgtatct
     1201 cgctccaggt tgtcgtcaga gatgacacct tatacaccag tcgcccctcc gcacaggggg
     1261 attcaccatg cgaatttttt taacgaatgc tcagtctgac aggcaactgt caactgactg
     1321 aattgtgaca cagattacac ttgttaccca cataccacga atcaggttat gcctcagtca
     1381 ttattaaact gcacttcagc aaatccggag cctgattcac aggtactgga tttgattgtg
     1441 acagtcattc ctgtcaactg agcactttgc agtaacggtt gcagattcca gcgactggtc
     1501 cagtactctt ttccggccac ttttactgtg aatgtatcat tctcattata cttggaaaac
     1561 tcaattttac ctttagcgca atccgccgcc attgcattaa cagaaactaa tgcaaataaa
     1621 accgccataa acatcttctt catacttaac tcctttattt acccgttgta tataaaaact
     1681 gtgactttct gttcagaaac gctgcagctg tattactttc ccataatgta ttgtttattt
     1741 ttataacggg cctgtcgcca gttatctgac attctggttg actatcttca ttcacggcgc
     1801 gaacagaacg cgccccctga tgatgacaat tcagtataac ggccacagtg cccagtatcg
     1861 ccgatatatt attaaaggat attctcccca ctctgacacc atcctctccc cgatactccg
     1921 gaagcacatt gctgattcgc ccccagttca gagtgaggtc cacgtctccc ggcgtcatcg
     1981 tatacacagg agcagtttca gacagtgcct gacgaaattc tctctgtatc tgcctgaagc
     2041 gtaaggcttc tgctgtgaca gtgacaaaac gcagaactgc tctggatgca tctctggtca
     2101 ttgtattacc actgaactcc attaacgcca gatatgatga aaccagtgag tgacgactga
     2161 tttgcattcc ggaacgttcc agcgctgcga cacgttgcag agtggtataa ctgctgtccg
     2221 ttgtcatgga aaccgttgtc acaccgggca ctgatatatg tgtaaaatct gaaaaacggt
     2281 agaaagtatt tgttgccgta ttaacgaacc cggccacata taaattattt tgctcaataa
     2341 tcagacgaag atggtcaaaa cgcgcctgat agacatcaag ccctcgtata tccacagcaa
     2401 aataactgcc cggtggggtg tggttaataa cagacaccga tgtggtcccc tgagatatat
     2461 gttcaagagg ggtcgatatc tctgtccgta tactatttaa cgaagataca taactttgtt
     2521 gagtcgaaaa gtctatcgta aattcccggg aataggatac cgaagaaaag cccagtaaca
     2581 ggcacagtac ccatttaaat aatatacact tcatatacaa ggtgttcctt ttggctgaag
     2641 taatcagcac cagacccggc gcagatataa aaaaggcccg caaaagcgag ccagggaaaa
     2701 taagtgtggc gcgttgtact ggattcgaac cagtgaccga ttgcttagaa ggcaattgct
     2761 ctgtcctgct gagctaacaa cgcagaatac cgataatgga ccaccaccgg ggactcgaac
     2821 ctcgcgcaac cagcttcgaa ggctgacgct ctatcccgat gagctaatgg cggttggtgg
     2881 cccttgctgg atttgaacca gcgacctggc gattatgagt cgctcgctct caccactgag
     2941 ctaaagggcc gagcgtagga taataacgtt acgaaatcaa tgttgcaaga gttcacaaat
     3001 tcactgattc aaaattattc ttgtaaaaaa tcttttaacg tcaggcacaa agagcctgta
     3061 catagatttg tgtaattgcc tgattttgat atgttcaatc cagcatcaaa tgaaggttaa
     3121 tttatggacg aaaaacagtt acaggctcta atgaccttta tacgattgac ccaaataaaa
     3181 aaagccaccg ttgcaactta agagtcacta acggcagctt atgcgaatag tgttgctcat
     3241 ttgctcaacg atgtcaacac gttctatgct acatgcttaa ttttctctac acgtttccgg
     3301 tttttaaacg cactatccag aatcgggtaa atcataaaca acgaggcatt gaggatttcg
     3361 tcaacttccc gtcggcaggt tgctagcgat ggtttttgaa tgcgcccgcc gccccggcat
     3421 aacatcttgc gaggtcttgc gacgctatga tagtaagatg caatggcgtg cttggaagag
     3481 ccgtgggcgt agtagctgag gaggatgcca aaggctttct tgtcaatgta catgacggaa
     3541 tcgacgacct gagaaatcaa cattccatca tcatcattac acattggcct tgtcataact
     3601 cttcccggct ccacgctctc catgaattta gctattacgc tgctcatgcg cttttccaga
     3661 cgacctgaat aaacccatgc gccccacagt tcaagccagc cattcagcca ctcatgctgt
     3721 tctttggtga ggtttagttc tcttatgccc acgcgccttc tccctgtacc tgaatcaatg
     3781 tgagatttcc gcagaacact gcgccggtat cgatatacat ctggttggca aatttgagtg
     3841 gtttcactgc t
//
LOCUS       DAZKIP010000239         3837 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZKIP010000239 DAZKIP010000000
VERSION     DAZKIP010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.241"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..142
                     /locus_tag="ACHOUK_004756"
     CDS             <1..142
                     /locus_tag="ACHOUK_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFP9900021.1"
                     /translation="GKNETITTPRLSFRFLNVSPTVERQLQRIIFSLEREAREKADKV
                     RD"
     gene            complement(144..755)
                     /gene="emtA"
                     /locus_tag="ACHOUK_004757"
     CDS             complement(144..755)
                     /gene="emtA"
                     /locus_tag="ACHOUK_004757"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFP9900022.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            855..1769
                     /gene="ldcA"
                     /locus_tag="ACHOUK_004758"
     CDS             855..1769
                     /gene="ldcA"
                     /locus_tag="ACHOUK_004758"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFP9900023.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQYDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNTFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILTNTREGTQLTISGHPVLKM"
     gene            1865..3601
                     /gene="cvrA"
                     /locus_tag="ACHOUK_004759"
     CDS             1865..3601
                     /gene="cvrA"
                     /locus_tag="ACHOUK_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFP9900024.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
BASE COUNT          812 a          962 c         1020 g         1043 t
ORIGIN      
        1 tggcaagaat gaaaccatca ccactccccg tctgagcttc cgttttctta acgtcagccc
       61 gacggtggag cggcaattac agcggattat tttctctctc gagcgggaag cccgggagaa
      121 agcggacaaa gtacgcgact gatttacatc gcgtccagtg cctgctcaag tttgtagata
      181 tagcgcggag cctgcggcgc aggatgattt cgcgctacgt gatcgaggaa ctcgtcagca
      241 tccagatcgt tgattttgct gattgccttt ttccgatctg acgagaaagt ccgtagcagc
      301 gcacctgccc cgttagcata tgacaccacc agcgcatatt gcagtacctt cggatcttca
      361 atgcctgcca gcgggccggt ttccagaata ttcaggtaag ccgcccccat agaaatatta
      421 cgctccggat ttttcagctc gctggtcgtc ggctcaccac tccagcccat acgccgataa
      481 acatcacgtc cggaggttga agcttttaac tgcatcaaac caatggcatt cgatttactc
      541 accgcgttcg gattaccacc cgattcgata gcgataatcg ccgtgatcaa ttgtggatcg
      601 acgccccagg ctgcaccggc tttctggctt attggcatcc actgcatcgc acgttgcacc
      661 ggaactttcg cgttccacgg cgggttcgta tagtcatgct ttgatgaaca acccgctaat
      721 aacacaatca aaaaggcaaa ccatctcaat ttcacttctt ctatccttat agcctggaat
      781 cgccgttgag gtgacaacgg cacacctaag cggcatgata tacatttcgt atctgttgtc
      841 agcaaggaat tgccatgtct ctgtttcact taattgcccc atcgggttac tgcattaaac
      901 agcacgccgc gctgcgtggt atccagcgcc ttaccgacgc ggggcatcaa gtcaataacg
      961 tcgaggttat tgcccgtcgt tgtgagcgtt ttgcgggtac ggaaacggag cgtctggaag
     1021 atctcaattc ccttgccaga ctgactaccc ccaacaccat cgtgctggct gtacgcggcg
     1081 gttacggtgc cagtcgttta ctggcagata ttgactggca ggcactggtg gctcgccaac
     1141 aatatgaccc gttgctcata tgcgggcata gcgattttac cgccattcag tgcggtcttc
     1201 tggcgcaagg caatgtcatc acctttagcg gccccatgct ggtagcaaac tttggcgcgg
     1261 atgaactgaa cacctttacc gagcaccatt tctggctggc gttacgcaat gaaaccttta
     1321 ccattgaatg gcaaggggaa ggcccgacat gtcgggccga aggcacgttg tggggaggca
     1381 atcttgcgat gctgatttca ctgatcggta caccgtggat gccaaaaatt gagaacggta
     1441 ttctggtgct ggaagatatt aacgagcacc ctttccgcgt cgaacgtatg ctgttgcagc
     1501 tctatcatgc gggaattttg ccacgtcaga aggcgattat tctcggtagc tttagcggca
     1561 gtacgcctaa tgattatgac gcaggttaca acctggagtc agtgtacgca tttttgcgtt
     1621 cccgcctgtc gattccactc attaccggcc tcgattttgg tcatgaacaa cgcacggtta
     1681 cgctgccgtt gggcgcacat gccattctta ctaacacccg ggaaggcact cagttgacga
     1741 ttagcggtca tcctgttctt aaaatgtaaa aaaaatcacg cttcagggct aagtaaaacg
     1801 ctgtctctgc cgctaatatg gtagggttta tataagagac agcgtaatca ggagtaaccg
     1861 accgttggat gccacaacaa taattagcct tttcatctta ggatccatcc ttgttaccag
     1921 cagtatatta cttagttcat tttcttctcg tcttggcatt cctattctgg ttatttttct
     1981 ggcgatcggc atgctggcgg gagtcgatgg cgtcggcggt atcccatttg ataattaccc
     2041 cttcgcctac atggtaagta acctggcgct ggcgattatt ttgctcgatg gcgggatgcg
     2101 cactcaggcc agttcctttc gtgtggcgtt aggaccggcg ctgtcgctgg cgacgctggg
     2161 cgtacttatc acctctggtt taaccggcat gatggcggcg tggctgttta atcttgattt
     2221 gattgaaggc ttattaatcg gcgctatcgt cggctccacc gatgctgcag cggtcttttc
     2281 tctgctgggt ggtaaggggc ttaacgaacg tgttggctcg acgctggaaa ttgaatccgg
     2341 cagtaatgat ccaatggcgg tctttctgac gattaccctg attgcgatga tccagcagca
     2401 tgaaagcagt gtcagttgga tgttcgtggt cgatattctg cagcaatttg gcctcgggat
     2461 tgtcatcggt cttggcggtg gttatttact gctgcaaatg attaatcgca tcgccctgcc
     2521 cgccggttta tatccattgc tggcattaag cggcggtatt ttaatttttg ctttaaccac
     2581 ggcgctggaa ggtagcggta ttctggctgt ttatctgtgc ggttttctgc tgggtaatcg
     2641 gccgattcgc aaccgctacg gcatcctgca aaatttcgac ggcctcgcct ggctggcgca
     2701 aatcgccatg ttcctggtgc tggggctgtt ggttaaccca agcgatctgc tgcctattgc
     2761 cattccggcg ctcattttgt ccgcatggat gattttcttc gcccgtcctc tttcggtatt
     2821 tgccggattg ctaccattcc gtggcttcaa tctgcgtgag cgcgtgttta tcagctgggt
     2881 gggattacgc ggcgcggtgc cgattattct ggccgtgttc ccgatgatgg cggggctgga
     2941 gaatgcacgt ctgttcttta atgtcgcctt ctttgtggtt ctggtttcac tgctcttgca
     3001 gggaacatca ctctcgtggg cggcgaaaaa agccaaagtg gtggttccgc ccgtcggacg
     3061 tccggtgtca cgcgttggcc tggatattca tccggaaaat ccgtgggagc agtttgttta
     3121 tcaattgagt gccgataaat ggtgcgtggg cgcggcactg cgcgatttgc atatgccaaa
     3181 agagacgcgt attgcggcac tgtttcgtga taaccagttg cttcatccta ccggcagcac
     3241 ccgactgcgc gaaggcgatg tgttgtgtgt aattggtcgg gaacgcgatc tcccggcgct
     3301 cggtaaactg ttcagccagt cgccgccggt cgcgctggat caacgcttct ttggtgactt
     3361 cattctcgaa gccagcgcca aatatgcaga tgtggcgctg atatatggcc tggaagacgg
     3421 gcgagaatat cgtgataagc aacaaacgct gggtgaaatt gtccagcagt tgttaggcgc
     3481 ggcaccggtt gtcggtgacc aggtagagtt tgccgggatg atctggacgg tggccgagaa
     3541 agaagataat gaagtgttga agattggcgt tcgagtggct gaagaggaag ctgaatctta
     3601 aatttaatga agcatctatc gccggttacg atgctttgct taacgcagcc tgagcctgta
     3661 tataaacttg tcatttcctg attttgatat gcacattcca acatcagaag aaggtttgaa
     3721 ccgccccggt tttcctggag agtgttttat ctgtgaactc aggctgccag atcatcgttt
     3781 ctgatggaag cataataagc tttttctgct tctgccggag ggatatgacc cagcctt
//
LOCUS       DAZKIP010000240         3797 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZKIP010000240 DAZKIP010000000
VERSION     DAZKIP010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.242"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            36..398
                     /gene="rusA"
                     /locus_tag="ACHOUK_004760"
     CDS             36..398
                     /gene="rusA"
                     /locus_tag="ACHOUK_004760"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFP9900025.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHVSAEGQAYRDNVARIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            395..535
                     /locus_tag="ACHOUK_004761"
     CDS             395..535
                     /locus_tag="ACHOUK_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFP9900026.1"
                     /translation="MMFEFNMAELLRHRWGRLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            621..1055
                     /locus_tag="ACHOUK_004762"
     CDS             621..1055
                     /locus_tag="ACHOUK_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFP9900027.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLIPEKVKSRPQ
                     CCDDDAMVICGCIARLYRNNRDLHDLLVDYYVLGETFMALARKHGCSDTCIGKRLHKA
                     EGIVEGMLMMLGVRLEMDRYVERELPGGRTSVFYQRKNSLRS"
     gene            1562..2509
                     /gene="stxA1a"
                     /locus_tag="ACHOUK_004763"
     CDS             1562..2509
                     /gene="stxA1a"
                     /locus_tag="ACHOUK_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
                     /protein_id="HFP9900028.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGTGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMAS
                     DEFPSMCPADGRVRGITHNKILWDSSTLGAILMRRTISS"
     gene            2519..2788
                     /gene="stxB1a"
                     /locus_tag="ACHOUK_004764"
     CDS             2519..2788
                     /gene="stxB1a"
                     /locus_tag="ACHOUK_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HFP9900029.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
     gene            3288..>3797
                     /locus_tag="ACHOUK_004765"
     CDS             3288..>3797
                     /locus_tag="ACHOUK_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFP9900030.1"
                     /translation="MTFKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVLVKPSDGEGTVISATRDTEYYFVVVLAGQSNSMAYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKK"
BASE COUNT          983 a          765 c          995 g         1054 t
ORIGIN      
        1 caggttatct ggctgaaaga gggggtaatc aaggcgtgaa tacctacagc atcacattac
       61 cctggcctcc gagcaataat cgctattacc gccataatcg cgggcgcacg cacgtcagcg
      121 cagaggggca ggcataccgc gataacgtcg cccgaatcat taaaaacgca atgctggata
      181 tcggcctggc tatgcctgtg aaaatccgca ttgagtgcca catgccggat cgccgtcgcc
      241 gtgacctgga taatctgcaa aaagccgctt ttgacgcact cactaaagca ggtttctggc
      301 tggatgatgc tcaggtcgtt gattaccgcg ttgtgaagat gcctgttacc aaaggtggga
      361 ggctggaact gaccatcacc gaaatgggga atgaatgatg tttgagttta atatggcaga
      421 acttcttcgc caccgctggg ggcgtctgcg cttatatcgt ttccccggtt ctgttttgac
      481 cgattaccga atactgaaga attacgccaa aaccctgaca ggagcaggag tatgaagtca
      541 gagataacaa tcaactaata ctgttttgtt gatttttgct tgtaattggc gttctggtct
      601 gatttttgtg gagtaagttg atgcgtgata ttcagatggt tcttgagcgt tggggagcgt
      661 gggcggctaa taatcatgaa gatgtgacct ggtcgtccat tgccgccggt tttaagggac
      721 tgatccccga aaaagtaaaa tcacgcccgc agtgctgtga cgatgatgcg atggtgatat
      781 gcgggtgtat agcccgcctt taccggaaca atcgcgatct gcatgacttg ctggttgatt
      841 attacgtgtt gggggagacg ttcatggcgc tggcacggaa acatgggtgc tctgacacct
      901 gtataggtaa acgccttcac aaagcggagg ggattgttga aggcatgctg atgatgctgg
      961 gagtgaggct tgagatggat cggtatgttg agcgtgaatt gccgggaggg agaacctctg
     1021 tattttatca gcgaaaaaat agtttacgat cgtaaaaatc tgcatatcat gataagagtg
     1081 gttacattgc cacgctgctt aacccgccga tgcgcgggtt tttttgtacc cagaatcctg
     1141 tgagctatac ggaaagtaca cagaaaggaa ggtgcgacca caattaataa caaaatctta
     1201 aaaattgcac atggcactat tagttttcta aatattgtgt attttttgta ttgcaggatg
     1261 accctgtaac gaagtttgcg taacagcatt ttgctctacg agtttgccag cctcccccag
     1321 tggctggctt ttttatgtcc gtaacatcct gtgtatcaat aaatgttgtt atctacgtac
     1381 gtcaagtagt cgcatgagat ctgaccagat atgttaaggt tgcagctctc tttgaatatg
     1441 attatcattt tcattacgtt attgttacgt ttatccggtg cgccgtaaaa cgccgtcctt
     1501 cagggcgtgg aggatgtcaa gaatatagtt atcgtatggt gctcaaggag tattgtgtaa
     1561 tatgaaaata attattttta gagtgctaac ttttttcttt gttatctttt cagttaatgt
     1621 ggtggcgaag gaatttacct tagacttctc gactgcaaag acgtatgtag attcgctgaa
     1681 tgtcattcgc tctgcaatag gtactccatt acagactatt tcatcaggag gtacgtcttt
     1741 actgatgatt gatagtggca caggggataa tttgtttgca gttgatgtca gagggataga
     1801 tccagaggaa gggcggttta ataatctacg gcttattgtt gaacgaaata atttatatgt
     1861 gacaggattt gttaacagga caaataatgt tttttatcgc tttgctgatt tttcacatgt
     1921 tacctttcca ggtacaacag cggttacatt gtctggtgac agtagctata ccacgttaca
     1981 gcgtgttgca gggatcagtc gtacggggat gcagataaat cgccattcgt tgactacttc
     2041 ttatctggat ttaatgtcgc atagtggaac ctcactgacg cagtctgtgg caagagcgat
     2101 gttacggttt gttactgtga cagctgaagc tttacgtttt cggcaaatac agaggggatt
     2161 tcgtacaaca ctggatgatc tcagtgggcg ttcttatgta atgactgctg aagatgttga
     2221 tcttacattg aactggggaa ggttgagtag cgtcctgcct gactatcatg gacaagactc
     2281 tgttcgtgta ggaagaattt cttttggaag cattaatgca attctgggaa gcgtggcatt
     2341 aatactgaat tgtcatcatc atgcatcgcg agttgccaga atggcatctg atgagtttcc
     2401 ttctatgtgt ccggcagatg gaagagtccg tgggattacg cacaataaaa tattgtggga
     2461 ttcatccact ctgggggcaa ttctgatgcg cagaactatt agcagttgag ggggtaaaat
     2521 gaaaaaaaca ttattaatag ctgcatcgct ttcatttttt tcagcaagtg cgctggcgac
     2581 gcctgattgt gtaactggaa aggtggagta tacaaaatat aatgatgacg atacctttac
     2641 agttaaagtg ggtgataaag aattatttac caacagatgg aatcttcagt ctcttcttct
     2701 cagtgcgcaa attacgggga tgactgtaac cattaaaact aatgcctgtc ataatggagg
     2761 gggattcagc gaagttattt ttcgttgact cagaatagct cagtgaaaat agcaggcgga
     2821 gattcataaa tgttaaatac atctcaattc agtcagttgt tgccggtctg ataatagatg
     2881 tgttagaaaa tttctgcatg gtgaatcccc ctgtgcggag gggcgactgg tgaacggtat
     2941 gatctctttg atgatcgtaa gcgagaatac gcgggtttgg tggcaccagg ccgaactcac
     3001 cgggaggcac ccggcatcat gctgtataca gagattaggc atatatccag gctcctcatc
     3061 gcaggagcct ttttacatgc aaaaaaaagc ccgagtgggt tcgggcaaca gcatgagata
     3121 cttgcattgt catttttatc gtgcggattt taaccaggat tcataaggct gcgcaactgc
     3181 gcggcctttt tcgtatttcg ggctgtagtc cccgtgtgtc attcaggctt ccggactaca
     3241 gcccactcca tatctgattt aatacactat cccggccggg aggaataatg acatttaaac
     3301 attatgatgt tgtcagggcg gcgtcgccgt cagaccttgc ggaaaagctg acacacaaac
     3361 tgaaagaggg ctggcagccg tttggtagtc cggtggccat cacgccttat actctgatgc
     3421 aggccattgc ggcggaaggt gatgtcacca cacctgtgtt ggtgaagccg tcggatggag
     3481 aaggcacagt aatcagcgcc accagagaca cggagtatta ctttgttgtg gttctggcgg
     3541 ggcagtcaaa cagcatggca tatggtgaag gccttccgct gccggagaca tatgaccgtc
     3601 cggacccgcg tattaagcag ctggcgcgcc gcagtacggt gacaccgggc ggtgcagcat
     3661 gcaaatataa cgacatcatt ccggcggacc attgtctgca tgatgtgcag gacatgagcc
     3721 gccttaacca tccgaaagcg gacctgtcaa aggggcagta cggaaccgtg gggcaagggc
     3781 tgcatatcgc caaaaaa
//
LOCUS       DAZKIP010000241         3775 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZKIP010000241 DAZKIP010000000
VERSION     DAZKIP010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.243"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(29..1066)
                     /locus_tag="ACHOUK_004766"
     CDS             complement(29..1066)
                     /locus_tag="ACHOUK_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFP9900031.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTKCHGESLRGHIDVTTEDLASALDVTEDVIDSILHAMQGRVLDGDLISGWEKRQV
                     MKEDNGNVSQTAKSPAERKRAQRERERLREQNTDCHDESRRVTHMSRQVTTDTDTDKE
                     LNPTHNARMRESAPASESNGAPLQTAEPEYPDGLSEPIGKFPMTGVWRPSLDFRQRAA
                     LWGMALPEPEFTPAELAAFRDYWMAEGKVFTQVQWEQKFARHVQHVRAQVKPVSKGVS
                     HAAPGGTASRAVQEIRAAREQWERENGFISNGNGLEAVGTYGGGVFEPLDPEERGCAV
                     EALDCSDWRDD"
     gene            complement(1135..1560)
                     /locus_tag="ACHOUK_004767"
     CDS             complement(1135..1560)
                     /locus_tag="ACHOUK_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFP9900032.1"
                     /translation="MKIKHEHIRMAMNAWAYPDGEKVPAAEIARTYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDAVEKMQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVAAAIAWGTLTNSGGQPGNAVVVH"
     gene            complement(1544..1867)
                     /locus_tag="ACHOUK_004768"
     CDS             complement(1544..1867)
                     /locus_tag="ACHOUK_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9900033.1"
                     /translation="MKKDNYSFKRACAVVGGQSAMARLLGVSPPSVNQWIKGVRQLPA
                     ERCPAIERATKGGVLCEELRPDVDWTYLRRSSCYSQNMSMKQPNDENDHTRSIKRQMI
                     HENQT"
     gene            1992..2468
                     /gene="racR"
                     /locus_tag="ACHOUK_004769"
                     /gene_synonym="rarC"
     CDS             1992..2468
                     /gene="racR"
                     /locus_tag="ACHOUK_004769"
                     /gene_synonym="rarC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor RacR"
                     /protein_id="HFP9900034.1"
                     /translation="MLSGKDLGRAIEQAINKKIASGAVKSKAEIARHFKVQPPSIHDW
                     IKKGSISKDKLPELWRFFSDVVGPEHWGLNEYPIPTPSTSDTKSELLDINSLYQAASD
                     EKRAIVAFLLSGNATEPSWVDHDVRAYIAAMEMKVANYLKNQESKRKSQNITKTGT"
     gene            2784..2936
                     /locus_tag="ACHOUK_004770"
     CDS             2784..2936
                     /locus_tag="ACHOUK_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFP9900035.1"
                     /translation="MDTLDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTESGARRW
                     LARNSD"
     gene            complement(3051..3566)
                     /locus_tag="ACHOUK_004771"
     CDS             complement(3051..3566)
                     /locus_tag="ACHOUK_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900036.1"
                     /translation="MIAELTAAMTAIRETAQIAKLMNEAKTQAEVNAAIGELNSKLAS
                     IQRECVSLVELVGTYQEINASLKAKIAEFENFEAQTEGYILSQLESGTFVYSKEVTVN
                     GGSIIMHLCPKCFGQKIVSILQPFPVREYEFFHKSRCLYCENQFLMNKNPDYVSPPSI
                     EELARKLNGNL"
     gene            3690..3775
                     /locus_tag="ACHOUK_004772"
                     /pseudo
     CDS             3690..3775
                     /locus_tag="ACHOUK_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          975 a          997 c          828 g          975 t
ORIGIN      
        1 ctgacggcag acctgccgga tttgctgttc agtcatcgcg ccaatctgag caatccagag
       61 cttcgacggc gcagccccgt tcttctgggt ccagcggttc gaatacacct cccccataag
      121 ttcccacagc ttccaggccg tttccgttgc tgataaatcc gttttcacgt tcccactgtt
      181 cacgtgctgc ccgaatttcc tgaactgccc gtgatgcggt gccacctggt gctgcatggc
      241 ttaccccctt gctgactggt tttacctgtg ccctgacgtg ctgcacgtga cgggcaaatt
      301 tctgctccca ctgaacctgc gtgaaaacct tcccctccgc catccagtaa tcccggaatg
      361 cggcaagctc agcaggtgta aactcaggct caggcagagc cataccccac aatgcagccc
      421 gctgtcgaaa atccagcgac ggccgccaga caccagtcat cggaaatttc ccgatcggtt
      481 cgctcaggcc atccgggtat tcaggttctg ctgtctgcaa cggcgcgcca ttcgactcac
      541 tggctggagc actctcgcgc atgcgcgcgt tatgtgtggg gtttaattct ttatctgtat
      601 ctgtatctgt cgtgacttgt cgtgacatat gcgtgacgcg tcgtgactca tcgtgacaat
      661 cagtgttctg ctcccgcagt ctttcccgct cccgctgcgc tctcttgcgc tctgccgggg
      721 attttgcggt ttgcgaaaca ttaccgttat cctccttcat cacctggcgt ttttcccatc
      781 cggaaataag atcgccatcc agaactcgcc cctgcattgc atgcaaaatt gaatcaatca
      841 cgtcttccgt cacatcaagc gcacttgcta aatcttccgt cgtgacatca atgtgaccac
      901 gtagtgactc gccgtgacat ttcgtgacat ttcgtgacgc actcaccaga aggtggatat
      961 acaccgccat cactgttgcg attggctgtc ctgagaccct ggcaatggtt cgccatttgg
     1021 gatcatttgg catgtcatgc cacaatctga gccaggcatt agccatactc acctcatctg
     1081 ataccgaact ttaccctcga acatccggaa gaaatccggc atgaatattg ttggtcaatg
     1141 cacgacaaca gcattaccag gctgaccccc actgttagtc agggtgcccc aggcgatcgc
     1201 cgctgcgaca aaatcatcca catctttcac cagccgatcc ctccgttcga cgatctcacg
     1261 gtaatattca gagctgtgac tgcgcatacg ggccaccagc agaggcggca ttgccttttc
     1321 gatcgccggt aacagagcct gcattttttc aacagcatca ggggtgtctt tatccagcca
     1381 acggaaaatt ttctgggtat tacgagccag ggcttccgga tggctgtcgt cgtacagttc
     1441 cgggaacgtc atccccagtt cgaaataagt ccgggctatt tcagctgcag gaactttctc
     1501 accatcagga tatgcccagg cattcatcgc catgcggatg tgctcatgtt tgattttcat
     1561 gaatcatttg cctcttgatg cttcgggtat gatcgttttc gtcatttggt tgcttcatcg
     1621 acatattctg cgaataacat gacgagcgtc gtaagtatgt ccaatcaaca tcaggacgaa
     1681 gttcttcaca caggacacca ccttttgttg ctcgttcaat cgcaggacat ctctcagcag
     1741 gcaactgacg tacacctttg atccattgat ttacgcttgg aggagataca cctaaaagcc
     1801 tagccattgc tgattgccca ccgacaacag cacaagctcg tttgaatgaa tagttatctt
     1861 ttttcatcga atgaactcca aaaaacacac aataatatta ggcttagcct aatgcaattg
     1921 tcaataggct atgcctaacg cttcgagagt aggtattgcc taatgcgatg cgcataggag
     1981 actattaagc aatgcttagt ggtaaagact taggccgagc gatagagcag gccattaaca
     2041 aaaaaattgc atcaggagcc gtcaaatcaa aggcggaaat cgcacgtcat ttcaaagtcc
     2101 aaccaccatc aatccatgac tggattaaga aaggttcgat aagtaaagac aaacttccag
     2161 aactatggcg tttcttttct gatgtggttg gtccagagca ttgggggctt aacgaatacc
     2221 ccataccaac cccatccact tcagatacaa aaagtgaact tttagacata aacagccttt
     2281 atcaagccgc ctctgatgaa aaaagagcaa ttgtggcttt cctcttatct ggaaatgcta
     2341 cggagcctag ttgggttgat catgacgttc gtgcctacat tgccgcaatg gaaatgaagg
     2401 tagctaacta tctgaaaaat caagaatcaa aacggaaaag ccagaacatc accaagacag
     2461 gaacttaaac ttatatggtc cgacgggaaa ttcctagttc ccgttagtta actcctacta
     2521 cctctcccac aaaccatcac ctattaggtc gcacccaaat cattaggcgt agcctattga
     2581 caagcaatta ggcatttcct atagttttcc cataccaacc catcccgtcc cacacaatac
     2641 agggcaatac ctagagttac ccggcagtgg tcagggatta agtagccagc ccgaggcgta
     2701 agaacatgac ggcagggttc aactttaact atgcagcagg tttttgttcc gctaccccgg
     2761 cgttaagggg atatgaggtc agcatggata ctcttgatct tggcaacaac gaatctctgg
     2821 tgtgcggcgt gtttcccaac caggacggca cgttcaccgc gatgacgtat accaaaagca
     2881 aaacgtttaa aaccgaatct ggcgcgcgtc gctggttagc aagaaactct gactaatgaa
     2941 atctggtaat taaggaatcc tccacggggg gagagtggtg cacacgcgcc ggacacaagc
     3001 aagcatccgg catgctcttt aacaatctgg atatccataa cagtaaaaat ctacagattg
     3061 ccgttcagtt ttctggccaa ctcttcaata gatggaggtg atacgtaatc cggattttta
     3121 ttcataagaa actgattttc acagtacagg cacctacttt tatgaaaaaa ttcatattct
     3181 ctaaccggga atggctgaag tatcgatact atcttttgtc caaaacattt tggacaaaga
     3241 tgcatgatta tgctgccgcc gttcacggtt acctccttcg agtacacaaa agttcccgac
     3301 tcaagttggc taaggatata accttccgtc tgagcctcaa agttttcaaa ttctgcaatt
     3361 ttagctttga gagaagcatt tatttcttga taagtgccca ccagctcaac gagagacacg
     3421 cattcgcgct gaatagatgc aagctttgag ttcagttcac caatagccgc atttacttca
     3481 gcttgagttt ttgcctcgtt cattagtttt gcaatctggg cggtttcacg aatagccgtc
     3541 attgctgccg ttaattcagc gatcacattc aatactctta ttgttgttgg ggatatccag
     3601 attaacaaga tccttgttgt tggggaataa cggggaccac ctcgcctgac gtggtaaaaa
     3661 gcaggcacac aacacgaaag cacacggcga gggacaccat taaatggtgg cttaagcaat
     3721 cacgcgaagc gcaatctgcc attatgaccg atgaaatccc gttagatgat gcact
//
LOCUS       DAZKIP010000242         3709 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZKIP010000242 DAZKIP010000000
VERSION     DAZKIP010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.244"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..901)
                     /locus_tag="ACHOUK_004773"
     CDS             complement(<1..901)
                     /locus_tag="ACHOUK_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFP9900037.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTRTPAPVGIFGPGWKAPSDI
                     RLQLRDDALVLNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWG
                     ALPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDVLPAPLPPYRVLTGMADRFGR
                     TLTYRREAAGDLAGEITGVTDGAGREFRLVLTTQAQRAEEARKQHTASLSSPDTPRPL
                     SDSAFPDTLPGTEYGPDRGIRLSA"
     gene            complement(968..3076)
                     /locus_tag="ACHOUK_004774"
     CDS             complement(968..3076)
                     /locus_tag="ACHOUK_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFP9900038.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSIDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     RGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKNYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAETIGNNQMIAVTNNQIQTVGVNQIETVGSNQIINVGSVQVETIGLVRA
                     LTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKTKKDD
                     TGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAA
                     SKSPPKTPDEKQDTPDMREY"
     gene            complement(3522..>3709)
                     /locus_tag="ACHOUK_004775"
     CDS             complement(3522..>3709)
                     /locus_tag="ACHOUK_004775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900039.1"
                     /translation="IGGAGENTILGTMNQVLLPLKQDHPGTRWWCSALEMVPSGELSL
                     FSMLFAYDIHQVNLINS"
BASE COUNT          806 a         1096 c          943 g          864 t
ORIGIN      
        1 ccgccgaaag gcggatacct ctgtcgggac cgtattcggt accgggcagt gtgtcgggga
       61 acgctgagtc tgaaagaggg cggggggtgt caggggaaga taaagaagcg gtgtgctgtt
      121 tacgggcctc ttccgcccgc tgcgcctgcg tggtcagcac cagacggaac tcccgcccgg
      181 caccgtccgt cacgccggtg atttccccgg ccaggtcacc ggcggcctca cgcctgtacg
      241 tcagcgtccg cccgaagcgg tccgccatcc cggtaagcac ccggtacggc ggcagcggcg
      301 ctggcagtac gtcctcagca cccggcaccc gctctgacca ccccagtatc caccacggcc
      361 cctgtgcgct gttggtcgcc aggtaaagat gcgggcttaa ccggatatcc ggcggcagcg
      421 ccccccacag ccgcgccagc gtgtggccgt ccggctgcgc tgccttacca ccgcgcacca
      481 gccacattga ctcgctgcgg ctgtacaccg cctcccccgg cagcagcggc tcaaagtgaa
      541 tgctccgccc gccgttgtca ttgagtacca gtgcatcatc gcgtagctgt aagcggatat
      601 cagaaggcgc tttccagccg gggccaaaaa tccccaccgg cgcaggcgtc cgggtccggt
      661 agctgctgta ggtgcgggag agaatgaacg gcagcgggcc gggcagcgca aggtccgtct
      721 cgccgggcag caccttcgcc cccagcagcg gatttaccgg gttgcccgaa gtcatcccgc
      781 ccgggcacac cgagcacgcc acgccagttg gcgcgccaat tcttacacct gccgaaccct
      841 ggacaatggg accgccatac tgagtcatat ctccctgacg cgccgctggt tttccgctca
      901 tgattcaatt ccttcttctg tttattttta atgcttgttt gttactgaat accgaacagg
      961 tacgggatca gtactctctc atatccggcg tatcctgctt ttcatcaggg gtcttcggtg
     1021 gggatttcga ggctgcacag ttccagttaa tcaacagtga gtcaccatta acctgctcct
     1081 taccctgcaa atgaattttt gtgccgttga gaaaaatttg tccgtccttc gtcagcacca
     1141 gccttgcctt accacagcag agctccagat gctcaccggc ggagtaaatc gcggtctgcc
     1201 cggtatcatc ctttttcgtt ttaccaacgg tgaaggtaac gttatttccg actttgacgt
     1261 cataacccag cccgaccctc aacgatttat gcaaaccgat ctgcgaggac tgcatcagag
     1321 ccaccgaagt gttcataatg ccacctaccg tcgtctggta cgccacgccc acggtcagcg
     1381 cacgaacaag tccaatcgtt tccacctgaa cagaacccac gttaatgatc tggttactgc
     1441 ccaccgtctc tatctggtta acgcccaccg tctgtatctg attgttggta accgcaatca
     1501 tctgattgtt gccaatggtc tcagcatggt tgtttatcac atccgtggtg cggttattca
     1561 gcacctcggt gttcatgttc ttctgcgcgt ggatgtagac ctgttctttc cctgtcgcat
     1621 cgtcaaactt cagttcatta aacccgctgc ccttatagtt tttcgaacga atggtcatct
     1681 gcgtcttcgt ccccggcagg ctgccgggtg tgcggttttc ctggtggtag gtacgcccca
     1741 taataatcgg ctggtccgga tcgccgttga ggaagtccac aatcacctcc tgacccacac
     1801 gcggtatcgc caggttgcca aagccggtgc ctgcccacgc ctgtgccaca cggatccagc
     1861 atgaactgtc ctggtttgac gggttatagc ggtcccagtt aaatttcacc cgcacgcgac
     1921 catgttcgtc gcagaagatt tcctcgcctg ccggtcccgt cacgacggcg ctctgcgggc
     1981 catccaccag cggtttcagc aacggctgtg gtcgccatgt ccggtctgcc ggtattaccg
     2041 caaagtggtt attcagcgtg gtacctgaac ccctgcgtcc cggcaccgcc tgtggctgct
     2101 cgccgtgcag ttcacttgcc accacctgcc attcccggtt caggttcgcc tgcggatgcc
     2161 ccgtcagcac aattcgccgt cccggccata tttccggaga acggcttgtt ccgcgcgcca
     2221 cttctgcgtt gttgcgccag ccatccatct gccagcgggc aaagttctgc ccgtgggcac
     2281 ccttgaaacg tccggggtag tcatacactt catactgtgt gcgctggtaa tcctggtact
     2341 ggccttcctg atcaaaacgc cctgcccagc cgggtcgttt aaaggtgtag tccttggtca
     2401 ccacggaaga agggcggatt tgtgcgctgt agcggaactg gctgatgcat agggtgctca
     2461 cctcggtacg ggtgttcggg ttccagggga tctcaaagga ctccggcaga taacgcacgg
     2521 tgtcgcacag gaccaggctc tggtcgatac ttttttgcgc gtgctcctca taaaagaaga
     2581 tgccttcctc cgccgccatc cggcacagga aatcgtaatc agtttcgccg tactggacac
     2641 aaaactcacg ggaaggatgc ggctcgctga acagcggact ccactcggtc acaccgtttt
     2701 ctttcaggat cgtagcgagt atgctttcga tgtcctcatt ctggaagata cggaagttct
     2761 ggcgcagccc tgtgcgccac agcggcgggc acaccttcat gctgtacagc atctggtttt
     2821 tgtcgttctc ccccagttca aaccaggtca ccacaccttt cacccggcgc tgtacgtcat
     2881 cgccctgcca taccgtcagg taggccattt tgtccagcac ctgcgcaaat tcaagggaaa
     2941 gaaactgctg actgaccaga gaaaggtcga gggaaaaaag tgaagagagt gactggttca
     3001 gatgaaagga gaccaccgca aacgcgtccg gtggcaggcc gtccacttcc agcgtgaaac
     3061 gtaatccggt tgacatagca acctccgtga aaatgaataa taatccgcac atattgcatg
     3121 tacaacatgt cgggcggatg aaataagtga gtcgacatct ggcgttaccg gggaatatca
     3181 gaaaaatgtc atggttatgt gatgatttag tctgggattt tacttcagag aaacgggtgt
     3241 ctgtttagtc aacaaaataa tcacaaacgc aacggatggg ttctcgacgt tattcacgac
     3301 aggctcgtac attacagcga gtaataaaaa acctgatatg caaagtcaaa ccaatattca
     3361 taatgtcttt ttattcacaa caataaataa tttaataaca atcattgaaa tgaatgatga
     3421 tttgtataag gaatatacgc taccgcccca ccctgataac atgatctgca cattgccatc
     3481 ttaacgtcgt taaacactgg cttaatagtg gatatttcaa attaggaatt tattaaattc
     3541 acctgatgaa tatcataagc aaatagcata gaaaataatg acaactcccc cgaggggacc
     3601 atttccaaag cactgcacca ccacctggtg cccggatgat cttgcttcag tggtaagagt
     3661 acctgattca ttgttcccaa aatggtgttt tcccctgccc ctccaataa
//
LOCUS       DAZKIP010000243         3708 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZKIP010000243 DAZKIP010000000
VERSION     DAZKIP010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.245"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(118..1275)
                     /gene="intS"
                     /locus_tag="ACHOUK_004776"
     CDS             complement(118..1275)
                     /gene="intS"
                     /locus_tag="ACHOUK_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFP9900040.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKRQVWSAAYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLAEAMKGYRKKNFPFLPADQIPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMQWKNVDFENRIITIEASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKSICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDGKVE"
     gene            complement(1437..1511)
                     /locus_tag="ACHOUK_004777"
     tRNA            complement(1437..1511)
                     /locus_tag="ACHOUK_004777"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1476..1478),aa:Arg,seq:cct)
     gene            complement(1587..2519)
                     /gene="yfdC"
                     /locus_tag="ACHOUK_004778"
     CDS             complement(1587..2519)
                     /gene="yfdC"
                     /locus_tag="ACHOUK_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFP9900041.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            2813..3568
                     /gene="mlaA"
                     /locus_tag="ACHOUK_004779"
     CDS             2813..3568
                     /gene="mlaA"
                     /locus_tag="ACHOUK_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFP9900042.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT          889 a          902 c          873 g         1044 t
ORIGIN      
        1 catataataa ttgcattttg ttattgttga tggtttcttg gtgtaagaaa tcctgagaag
       61 tgaaaagctt tgatatatgt gcaaggcttt tcttttttat aagaatcgtt gtaataacta
      121 ctccaccttc ccatcaagcc agtccgccca ccactgcatc atttctctgc gcttatcgag
      181 atactgagca tggttgtaaa tcccacgcac agatccgccg ttggcatgtg ctagttgcac
      241 ttcaatagcg tcagcaggcc attcgtgctc gttcataatc gtgctgaatt catgcctgaa
      301 tccgtgaccg ctttccagac cttcatagcc gatttgtttg atcacaagca gtacagcgtt
      361 ctcacagatt gacttcttct tatcgttgcg cccggcaaaa acaaactctg atactggttt
      421 agtgatggag cttagcgtag tgagaagttc aacaacctgg tccgacatcg gaaccacatg
      481 aatcttgcgt cccttcatca cactggcctc gatggtgata atcctgtttt caaaatcgac
      541 gttcttccat tgcatggaac gaagctcttt cgttcttagt gctgtgtagc gtaaaacttt
      601 ggtcgcaatg agagatacga tacttcctga aaatgttgca agtgctttgt tgaatgccgg
      661 gatctggtcg gcaggtaaaa acgggaagtt cttcttgcgg tatcccttca tggcttcagc
      721 aaggtcaggt gccgggttat atttagccct tccggtgaca atagcgtaac ggaaaacctc
      781 gccgcatctt ctgcgtgctt tgttggctcg ctccattgcc ccgcgatctt caaatctgcg
      841 gattacttcc agcagttgca tcggctcaat atcctgaatt tcaaggccgc cgatgatagg
      901 taaaatgtcg tcatcaaaca tttttgcaag ttcagtcgca taggcggctg accagacttg
      961 cctcttatgc tcgtaccatt ccttgtaaat cgcactaaag gaattgttgt tagacgaagc
     1021 ctttttcgcc tttaccggat cgatgccaac cgagatgtct ttcctcgcag tccatgcttt
     1081 atcccttgcc tcttgcaaag tcataagcgg atattttccg acggtcagga ttttctcctt
     1141 accgtcaatc ttgtagcgaa gctgccatac ctttttccct gatacaggga cataaaggta
     1201 caggccatta ccatcgagta ggcggtatgg tttttctttc ggctttgctg cttcaatctg
     1261 cttaacggtg agcatgggta aaaatccggt gggtaaaatt attttatcca ctttttaccc
     1321 gtcatggcgt gcggctgtca acgatctgac gcgaaccatg acgaactgtg aatctacgga
     1381 aggcttgata ttcaggggat tttgcggact ggtacggatg gaggcgaact gataaatggt
     1441 gtcccctgca ggaatcgaac ctgcaattag cccttaggag gggctcgtta tatccattta
     1501 actaagagga caatgcggca tgagtatacc cgctaatgga ctgcggggta agtacgctgc
     1561 cgctcgattg cttaaaccct cgccatttat gccgggtttt tatcattttt cttaatgttt
     1621 tccgcacgtt ctgctttttg gcgtgcttct gctttgcgct tgttgctcat gtcgttacgg
     1681 atctgtgcat gactcattaa cgcgaagata aaggtgccgc cgcagatgtt ccccgctaaa
     1741 gtaggtagtg cgaagggcca gatgaaatcg ctccagtgca gcgtaccgtt aaacaccaga
     1801 tagaggattt caacagaacc gacaacgatg tgagtggtgt cacccagggc aataagccag
     1861 gtcatcaata taatcaccac aatctttgcc gcacccgctg caggaaacat ccataccata
     1921 gtggcgatca gccagccgga aatgatcgcg ttggcaaaca tctcgctggg ggtgttcttc
     1981 atcacatcca tgccgatttt gacaaatgca tcgcgagttt cttcattgaa gattggcata
     2041 tattcaaatg cccacgccgc aatacctgtc ccgagaatat tacccagcag cacgacgccc
     2101 cataaccgca taagtaagcc aacgttgctc attgtcggtt tttgcatgac gggtagtacc
     2161 gcagtcacgg tgttttcggt aaataattgc tggcgggcca taatgacgat aataaaacca
     2221 aaggtataac cgagattctc cagcaagaag ctgcccggca caccttccag ttcgacatga
     2281 aatatccctt ttgccagtaa tgaagcgccc atcgacagac ccgccgcaat ggctgaccac
     2341 agtagtgcca ttgcgtcgcg ttccagctct ttttcaccat cctggcggat atgctcatga
     2401 attgccatcg cccgggaggg gagtcgatct tcatctattt ctattttttt gccacgctct
     2461 ttttcttcgc tctcaacttc aatttcgtcg ctgtgttgat caattttgtc gttgtccatg
     2521 gtctcttctc ttgaattagc acgtatagct aaagcgtagc ggcttttttg ctcgcaactg
     2581 gcgggagtta ctttgaaaat gtagaaaagg ccgcgtttgc ctttttctgt ttctatagaa
     2641 tcaagtagcc tacagggcgg cgattaccag gctatgatca aatcagcaaa tcagggcgtc
     2701 tggacatcag ttgacgtgct gttacaatcg cccacaccta aacaggcgga tacggtatcg
     2761 ttccgtcatg gatggcaaac tgcataagcc ataaaaaaac agggagacat ttatgaagct
     2821 tcgcctgtcg gcgcttgctc tgggaactac gcttctggtg gggtgtgcga gttccggtac
     2881 agatcagcaa gggcgttctg acccgttaga agggttcaac cgcaccatgt acaactttaa
     2941 cttcaatgta ttagacccgt atattgttcg accggtcgct gtcgcctggc gtgattatgt
     3001 tccgcaaccg gcgcgtaacg gtttgagcaa ttttaccggc aaccttgaag aacctgcggt
     3061 gatggttaac tacttcttgc agggcgaccc ttatcagggg atggtccact ttacccgctt
     3121 tttcctgaac accattttgg ggatgggcgg ctttattgat gttgcaggga tggcgaaccc
     3181 gaaactgcaa cggactgaac ctcaccgctt cggtagtacg cttggtcatt atggcgtggg
     3241 ttatggcccg tacgttcaat taccgttcta cggtagcttc acgctgcgtg atgacggtgg
     3301 tgatatggcg gatggtcttt acccggttct ttcctggctg acctggccga tgtctgtggg
     3361 taaatggacg cttgaaggta tcgaaacccg cgcacagctg ctggattccg acggtctgct
     3421 gcgtcagtcg tccgatcctt atattatggt gcgcgaagcg tacttccagc gtcatgattt
     3481 catcgctaat ggcggcgaac tcaaaccgca ggaaaatccg aacgcgcaag cgattcagga
     3541 tgatttaaaa gatatcgatt ctgaataaga aacaaataaa aaaggtgagt cgcaaaactc
     3601 accttttttg ttgttatccc tcaattacca caagtaaaag gagataaacc gacaatgacc
     3661 cggacgtaat tatgtaaaaa tactgatgtt cgtcactgac tatttccg
//
LOCUS       DAZKIP010000244         3643 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZKIP010000244 DAZKIP010000000
VERSION     DAZKIP010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.246"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            435..1178
                     /locus_tag="ACHOUK_004780"
     CDS             435..1178
                     /locus_tag="ACHOUK_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP9900043.1"
                     /translation="MATTCSVILILESFDVYFGKESVFLERGSSVLVDSSSRDFFLTY
                     PERVIVADFGAEFISRYLKANNLRDISDCREYPSYLKINFADFSLIKGLISWANHCAE
                     YIEIFDESIAFTCLSAFSSEKQFGVFLFGCLKSTGAKVKTIIHTDLSAPWRLKDISSR
                     LYLSESLLKRKLKEEGVSFSKIILDERMQMAEYLLSTRCYPISKVAKVCGYASVSYFT
                     YVFRRYFGVSPSQYSQRSSESKILTHQGI"
     gene            complement(1220..1654)
                     /locus_tag="ACHOUK_004781"
                     /pseudo
     CDS             complement(1220..1654)
                     /locus_tag="ACHOUK_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612066.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            2141..3322
                     /locus_tag="ACHOUK_004782"
     CDS             2141..3322
                     /locus_tag="ACHOUK_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9900044.1"
                     /translation="MPLNDMQIRRAKPEAKAYTFGDGLGLSLLIEPNGSKSWRFRYRY
                     AGKPKMISLGVYPTITLADARSRRDEARKLVAEGKNPSEVRKEQKLAMQTESENAFEK
                     IAREWHQLKSAKWSAGYASDIMEAFKNDIFPYVGTRPVGEIKPLELLNVLRKIEKRGA
                     LEKMRKVRQRCSEVFRYAIATGRAEYNPAADLSSALEVHQSNHFPFLKADEIPDFLRA
                     LEGYSGSKLVQIATKLLMITGVRTIELRAALWQEFDLDNAIWEIPAERMKMRRPHLVP
                     LSSQAVDLLNELKIMTGNYRYVFPGRNDPNRPMSEASINQAIKRIGYGGKVTGHGFRH
                     TLSTILHEQGFESAWIEIQLAHVDKNSIRGTYNHAQYFSGRKSMMDWYSNLIFERLKR
                     S"
     gene            3326..>3643
                     /locus_tag="ACHOUK_004783"
     CDS             3326..>3643
                     /locus_tag="ACHOUK_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900045.1"
                     /translation="MSQPLSEILTWDDEQWEVFVHDWLIVCKSDDYPWSERLGGAGDK
                     GRDVVGYKSDPNVEGYSWDNYQCKLYKKSLGFSDVVVEFGKLIYFTLNGDYPIPQKYF
                     FVAP"
BASE COUNT          990 a          680 c          843 g         1130 t
ORIGIN      
        1 ttttgagcga aaatctgcag ttattcagtc gttttcttat cggtcaccat tattctttta
       61 gacattgatc ctacaaagct gccgcaaagt tggtggtggg aactgaagtt gcgtagagaa
      121 ggggtcaata cccggaggca aacatgggct ggcaaaagtg tagcggtatt aggcgcagct
      181 atttagccta gttatgtttt atgaaaactt gatatcatat aagtgtctta cttattggct
      241 gtaaataagt ttttcctaag gaattgtttc ttgagtatca tttgtaactg taacggaatt
      301 tataatcctt tgctttattg ttacggtatt ttttatcaca ccctattttt agtggttttt
      361 tatactgaag tttggcaaag tgaactttat atacatatac ttcatcctgg tttcagttaa
      421 attgggggga tgatatggca actacatgtt cagttatatt gattttggag tcctttgatg
      481 tttatttcgg aaaagagagt gtgtttctgg agagaggttc atctgtactt gtcgactcta
      541 gctctagaga ttttttcctg acatatcctg aaagagtgat agtggcggat tttggcgctg
      601 agtttattag tcgctatttg aaagctaata acttaaggga tatttctgat tgtagggaat
      661 atccatctta tttaaaaata aactttgctg acttcagttt aattaaagga ttaattagtt
      721 gggctaatca ctgtgctgaa tacatagaaa tttttgatga gtctattgct tttacatgtc
      781 tctctgcatt ttcttctgaa aaacaatttg gagtatttct gtttggatgt ttgaaaagca
      841 caggggctaa agttaaaacg attattcata cggatttatc tgcaccatgg cgtcttaagg
      901 atatatcatc aagattatat ctcagcgaaa gtttactaaa gaggaaattg aaagaagagg
      961 gggtatcatt cagtaagatc atacttgatg agaggatgca aatggctgaa tatttactca
     1021 gcactcgttg ttatcctatt agtaaagtag ctaaggtctg tggttatgcc agtgtctcat
     1081 actttactta tgtatttaga cgttattttg gtgtttctcc aagtcaatac tctcagagga
     1141 gttcagaaag taaaattctt actcaccagg gaatctgatc attgttcttg cccccttatt
     1201 tccagacagg gggggtatct taagttaacg ttacccgctg acgtcgatat tctcgcggag
     1261 agcgataacc caacgcactg tgcggatggt tttcattgta atgttcgatc gccactgcaa
     1321 gattatgcaa tgccgttctt acattcggtt tcggcatgaa cgcgatgtag tcttccttca
     1381 tcgttttcac gaacctttct gcaattccat tactctgagg actgctgatt gccgttgtgc
     1441 agggctccag attcaactct ctggcgaact gccgtgtttc atgcgctcta tacgctgaac
     1501 cgttatctgt cagccactgg atggactgtt ccggtacttt atcgccaaag cgtttttcta
     1561 tcgctcctaa catgacatcc tgcactgtcg ctttatcgta acctcccgtg cttgcggccc
     1621 agtctatggc ttcacgatcg caacagtcca gggcgattca tctgggccaa tccttgctca
     1681 taacaggcat tcacttcagt catggcagaa aggtatgcat gctggagaag tcgtgaaaga
     1741 aaagaagact gctgcgccgt ttgtcgtcac gtttatcttc attggctatg caagtcgtaa
     1801 tacaaggtgg gacaaaactg agacacataa ggcctcgcaa tggcttgcaa ggctttacat
     1861 gttttgatgt ggtgggacgt gtgagcgcag tgttgatggg gtaatgcttt gaattagaag
     1921 cggattctta taattcgtaa tgcgaaggtc gtaggttcga ctcctattat cggcaccatc
     1981 tcaacttcct caaacgtccg tattagtcca taaaatctct gatttataac attttttatt
     2041 cttttaagtc catagtagtc cgtaaccatc cagtagaatc cggtactgaa tgtgtatagg
     2101 attgtgtata tgttcctgtt cggtctggat tcctatacac atgcctttaa acgatatgca
     2161 gattcgccgc gctaagcctg aagctaaagc ctatacattt ggagatgggc tagggttgtc
     2221 attacttata gaacctaatg gaagcaagag ttggcggttc cgctatcgct atgccggcaa
     2281 acccaaaatg atctcgcttg gtgtttaccc aacgatcacc cttgccgatg ctcgttcccg
     2341 tcgtgatgaa gctcgaaaac ttgtggcaga aggaaagaac cctagtgagg ttcgaaaaga
     2401 gcaaaagcta gctatgcaaa cagagtcaga gaacgccttc gaaaagatag ccagagagtg
     2461 gcatcaactt aaatctgcta aatggtcggc gggatatgca tcagacatca tggaagcgtt
     2521 taagaacgac atttttcctt atgtcggaac aaggcctgtg ggagagatta aaccgctaga
     2581 gctgctgaac gttctgcgta aaattgagaa acgtggtgcg ttggagaaaa tgcgcaaagt
     2641 gcggcagcgt tgctccgaag tgtttcgcta cgcaattgca acgggtaggg cggagtacaa
     2701 tcctgcggct gatctctcca gcgctctcga agtacaccaa tccaatcatt tcccattcct
     2761 aaaagctgat gagatacctg attttctacg tgccttagag ggttactccg ggagtaagct
     2821 tgtccagata gccacgaaat tactgatgat tacgggtgtg agaaccatcg aattacgcgc
     2881 ggcattatgg caagaatttg atctggataa cgctatttgg gaaattcctg ctgaaaggat
     2941 gaaaatgcgt aggccacatc ttgtgccctt atcatctcaa gcggtagatt tactcaatga
     3001 actcaagatc atgacaggga actatcgtta tgtttttcca gggcggaacg atccgaatag
     3061 gccaatgagc gaagcgagta taaatcaagc cattaagcgt attgggtatg gaggaaaagt
     3121 cactggacat ggttttcgtc ataccctttc tacaatcctg catgagcaag gttttgagag
     3181 tgcttggatt gaaatccagt tggctcatgt agataaaaat tctattaggg ggacttataa
     3241 ccatgctcaa tattttagtg gaaggaagtc tatgatggac tggtacagta atttgatatt
     3301 tgaaagacta aaaaggagtt aatttgtgtc tcaaccactt tcggaaatat tgacatggga
     3361 tgatgaacaa tgggaggtat ttgtccatga ttggcttatt gtctgtaaat cagatgatta
     3421 cccgtggagc gaacgtttgg gaggagctgg agataaaggt agagacgttg ttggatataa
     3481 atcggatcct aacgtagaag gttattcttg ggataattat caatgcaaac tgtacaaaaa
     3541 aagtttaggg ttctctgatg ttgtagttga gtttggaaaa cttatctatt ttactctgaa
     3601 tggtgattat cccatccctc agaagtactt ttttgtggca ccc
//
LOCUS       DAZKIP010000245         3642 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZKIP010000245 DAZKIP010000000
VERSION     DAZKIP010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.247"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..845)
                     /locus_tag="ACHOUK_004784"
     CDS             complement(<1..845)
                     /locus_tag="ACHOUK_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFP9900046.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPYGGPVAITPYTLM
                     QAVAIEGDPQVGPSSKPDWFYVVVLAGQSNGMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGESCTYNDIIPADHCLHDVQDMSTLNHPKADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGAEGTFSADTGASQDSARWGVGKPLYQDLIARTKAAL
                     QKNPKNVLLAVCWMQGEFDMSAATHAQQPALFTAMLTQFRADLSVFNAQCHGGSAADV
                     PWICG"
     gene            1114..1230
                     /locus_tag="ACHOUK_004785"
                     /pseudo
     CDS             1114..1230
                     /locus_tag="ACHOUK_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1335..1411)
                     /locus_tag="ACHOUK_004786"
     tRNA            complement(1335..1411)
                     /locus_tag="ACHOUK_004786"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1375..1377),aa:Arg,seq:tct)
     gene            complement(1425..1501)
                     /locus_tag="ACHOUK_004787"
     tRNA            complement(1425..1501)
                     /locus_tag="ACHOUK_004787"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1465..1467),aa:Arg,seq:tcg)
     gene            complement(1509..1584)
                     /locus_tag="ACHOUK_004788"
     tRNA            complement(1509..1584)
                     /locus_tag="ACHOUK_004788"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1549..1551),aa:Ile,seq:cat)
     gene            complement(1613..2326)
                     /locus_tag="ACHOUK_004789"
     CDS             complement(1613..2326)
                     /locus_tag="ACHOUK_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9900047.1"
                     /translation="MAVVCSVIMVCSPINIFLEKDTLSLKPGSVVLATKCIRELFLMH
                     YGKVKIVDISESVVSQYLESQHKLTRTRLTDIPLYLLLEPNNPALAAALITSQGFSGE
                     VTDMFLMMACLSLFETDERMSLFLSGCLSSISAKVRAIIQTDISASWTLGAIALQLHM
                     SESLLKTKLKNEGGMFSRLLLEERMRVAVNMLCSRYGYGQAVAEKCGYSSRSYFISVF
                     HRYYGFPPDRYVSRQGLDY"
     gene            complement(2462..2659)
                     /locus_tag="ACHOUK_004790"
     CDS             complement(2462..2659)
                     /locus_tag="ACHOUK_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFP9900048.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQCGCYRRIQS"
     gene            complement(2884..3438)
                     /locus_tag="ACHOUK_004791"
     CDS             complement(2884..3438)
                     /locus_tag="ACHOUK_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFP9900049.1"
                     /translation="MIYPEITGKSGEHLRLKTLESVWIQGKLRMWGRWSYIGDGKTGN
                     MFNQLLTSKKLTKTAINEALRRMKKAGLDKPELEAFLRDMINGNQKSWLAHCTDSEAL
                     IIDRVIGEVLAGYPGLLNVLSQRYVGRGMTKRKMAELLNDAHPEWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
     gene            complement(3501..>3642)
                     /locus_tag="ACHOUK_004792"
     CDS             complement(3501..>3642)
                     /locus_tag="ACHOUK_004792"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP9900050.1"
                     /translation="IKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIV
                     RG"
BASE COUNT          935 a          997 c          798 g          912 t
ORIGIN      
        1 tcaccacaaa tccacggcac atctgcagca ctgccaccat ggcactgcgc gttaaacacg
       61 gagaggtcag cacgaaactg tgtcagcatg gctgtaaaca gcgcaggttg ctgtgcgtgg
      121 gtggcggcgc tcatgtcaaa ctctccctgc atccagcaca ccgccagcaa cacatttttc
      181 gggttcttct gtaatgcagc tttagtgcgc gcaatcaggt cctgatataa cggtttaccc
      241 acaccccagc gtgccgaatc ctggctggcc cccgtgtccg cactgaatgt cccctcagcg
      301 ccctgggtga atgcagaacc accacgacag catggtacca gcaggatccc cgcgttattc
      361 gggatatacg gaagcagttt tttggcaata tgtaagccct ggccgacaca gccgtactgc
      421 cctttgctca ggtctgcctt cggatgattc agcgtactca tatcctgcac atcatgcagg
      481 cagtggtcgg ccggaatgat gtcgttatac gtacaactct ctccacccgg agttaccgtg
      541 ctgcggcgcg ccagctgttt aatgcgcgga tccggagcat cgtaagaatc cggtaacgga
      601 agcccttcac cgtaggccat gccgttggac tgtccggcaa gcacaaccac gtagaaccag
      661 tccggcttag atgaagggcc gacctgtgga tctccttcaa tagccaccgc ctgcatcagt
      721 gtgtacggcg taatggcaac cggtccgccg tatggctgcc agccctcttt cagtttgtgt
      781 gtcagctttt ccgcaaggtc tgacggcgac gccgccctga caacatcata gtgtttaaat
      841 gccatgaatc ctcccggccg ggataatatt gtgagtaaaa tgaggagcgg gctgaagtcc
      901 ggaagttaca ggacaatggc agaagagaga caacagcccg caatacgaaa aaggccgcgc
      961 tattgcgcag agtgattact gtcggatatt attcgccagc tgaaatatta cttcacgttt
     1021 tgttgtttat tccttgccgc ccgcgtctcc ctgcgcgggc tttttttgtc cataagaaag
     1081 cccctccgga gaggggctgg agagtggcgc tatgtgccat tgcatggtgc cgggtgcctc
     1141 ccggtgaatt cagtaccagc acctgaatcc gcgattatcc catataccta ctcgctgatt
     1201 gcccctccgc acagggggat tcaccatgcc agtttctttt aacaaactcc ccgcaaacca
     1261 gacaacagtc aaccgcctga attgtgagac atttaaaaaa aaggcccgca aaagcgagcc
     1321 agggaaaata agtgtggcgc gttgtactgg attcgaacca gtgaccgatt gcttagaagg
     1381 caattgctct gtccggctga gctaacaacg caggatacag ataatggacc gccttcgggg
     1441 acccgaactc cgcgcaacca gcttcgaaag ctggcgctct ttcctgatga gctaatggcg
     1501 gtatgtgatg gtggcccttg ctggatttga accagcgacc tggcgattat gagtcgctcg
     1561 ctctcaccac tgagctaaag ggctgggtca aaaaataata atcagatgaa aatcaataat
     1621 caagcccttg cctggataca tatctgtctg gcgggaagcc ataatagcgg tgaaatacag
     1681 aaataaagta ggaccggctt gaataaccac atttttctgc tacagcctgt ccatatccat
     1741 accgggaaca taacatattt acagcaacac gcatccgctc ttccagcaac aagcgactga
     1801 acatgccccc ttcatttttc agttttgtct ttaacaaact ctcactcata tgcaactgta
     1861 gagcaatcgc accaagcgtc cagcttgctg atatatctgt ctgaattatc gccctgactt
     1921 tggcacttat gctggataaa catccactta aaaacaatga catccgttca tctgtttcaa
     1981 acagagacag gcaggccatc ataagaaaca tatccgtgac ctctccggaa aatccctggc
     2041 tggtaattaa agccgcagcc aacgcaggat tgttgggttc cagcaacagg taaagcggaa
     2101 tgtcagtcag acgagtcctc gtcagcttat gctgactttc cagatattga cttacgacgg
     2161 attcgcttat atcgacaatt ttaactttgc cataatgcat aaggaaaagc tccctgatgc
     2221 atttggtggc cagaacgact gagcctggct taagtgacaa cgtatccttt tcaagaaaaa
     2281 tattaattgg ggagcaaacc atgataactg aacagacaac agccattata attttacttt
     2341 cattagcaat tggttagctc aattatagcc ccaaaaggta aattatcatc aacacataag
     2401 caaaggactg acaggtgccg ccccccccac cagtcgccca tttaccacaa ataaaaagcc
     2461 ttcaggactg aatgcgtctg taacaaccgc actgatagtc tgccagaccc gccataacca
     2521 gctgggtcag tattaactgg cagcgttcgc gtgaaaggta agtattctgc gcaatctccc
     2581 cgactgtcgc cggttcggta acgcttaatt cattaaacac cactctggcg gtttctgtca
     2641 tatcctgctg ttttagcatg tctttttccc ttttccggtt aacgtgacat accaataact
     2701 cttgtcgaaa aagccagcaa gttgaaagac cggtattagc aaccaccagc gcgtttaacg
     2761 ccccgtgccg tttttcagtc ataaaaaaac ccgcaaaaag cgggctcttt caaatgtcca
     2821 tgtctgctat tcgcctcgcg gtacagcttt gcgaagctta ccggaattga agcagttttt
     2881 acgtcaaaaa gcaataactt ttttctctat accaaaagcc ataaccattg gtttgtacaa
     2941 aataaattct gccaccttta gccaatgctc aatgcgtctt tcacaggttc ttaaactcca
     3001 ttccggatgt gcatcattca gcaattcagc cattttgcgc ttagtcatcc cccgccccac
     3061 ataacgctga ctcagaacat tgagcagccc gggataacct gccagtactt caccaataac
     3121 cctatcgatt attaacgcct ctgaatcggt acaatgtgcc agccagcttt tttgattgcc
     3181 gttgatcata tcccgcaaaa aagcctcaag ttcaggtttg tccagacccg cttttttcat
     3241 cctccggagc gcctcgttaa ttgccgtttt tgtcagcttt ttagaggtca gtaattggtt
     3301 gaacatattt cccgtcttac cgtcgccaat atacgaccaa cgcccccaca tacgcagttt
     3361 cccctggatc cagacacttt ccagcgtttt caggcgtaaa tgctcaccgc ttttgcctgt
     3421 aatttccggg tatatcatat ttatgctcac tcactttcaa ttttgtaaat cttcacgccc
     3481 agccgcccac cagaaacgag ctagccgcgc acaatattga tttcatcaaa ctgctcgtcg
     3541 tctatgagaa gtccggcatg cgtcagcgca tccagtggtg ctttcaggat attgtccagg
     3601 tcacgacggc gcttatccgg tggctctgca ataatcttta tc
//
LOCUS       DAZKIP010000246         3589 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZKIP010000246 DAZKIP010000000
VERSION     DAZKIP010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.248"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..155)
                     /gene="escO"
                     /locus_tag="ACHOUK_004793"
     CDS             complement(<1..155)
                     /gene="escO"
                     /locus_tag="ACHOUK_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase activity
                     positive regulator EscO"
                     /protein_id="HFP9900051.1"
                     /translation="MAKINSQIKEVDGKLDDCEQSIKESIASKQAYCASLVNLDKVSL
                     YKYQIKN"
     gene            complement(215..1555)
                     /gene="escN"
                     /locus_tag="ACHOUK_004794"
     CDS             complement(215..1555)
                     /gene="escN"
                     /locus_tag="ACHOUK_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase EscN"
                     /protein_id="HFP9900052.1"
                     /translation="MISEHDSVLEKYPRIQKVLNSTVPALSLNSSTRYEGKIINIGGT
                     IIKARLPKARIGAFYKIEPSQRLAEVIAIDEDEVFLLPFEHVSGMYCGQWLSYQGDEF
                     KIRVGDALLGRLVDGIGRPMESNIAAPYLPFERSLYAEPPDPLLRQVIDQPFILGVRA
                     IDGLLTCGIGQRIGIFAGSGVGKSTLLGMICNGASADIIVLALIGERGREVNEFLALL
                     PQSTLSKCVLVVTTSDRPALERMKAAFTATTIAEYFRDQGKNVLLMMDSVTRYARAAR
                     DVGLASGEPDVRGGFPPSVFSSLPKLLERAGPAPKGSITAIYTVLLESDNVNDPIGDE
                     VRSILDGHIVLTRELAEENHFPAIDIGLSASRVMHNVVTSEHLRAAAECKKLIATYKN
                     VELLIRIGEYTMGQDPEADKAIKNRKLIQNFIQQSTKDISSYEKTIESLFKVVA"
     gene            complement(1539..3566)
                     /gene="escV"
                     /locus_tag="ACHOUK_004795"
     CDS             complement(1539..3566)
                     /gene="escV"
                     /locus_tag="ACHOUK_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscV"
                     /protein_id="HFP9900053.1"
                     /translation="MNKLLNIFKKAESYHDLILALFFFMAVMMMIIPLPTVVVDIIIA
                     INISTALLLLMLSIYIKNPLELTSFPTILLITTLMRLSLSVSTTRLILLHHDAGDIIY
                     SFGNFVVGGNIVVGLVIFTIITIVQFMVITKGAERVAEVSARFSLDGMPGKQMSIDGD
                     MRAGVIDPLEAKVLRSRVQKESQFYGSMDGAMKFVKGDAIAGIIIVLVNLFGGVLIGM
                     WQFDMPFSAALSLFSVLSVGDALVAQIPALIISVTAGVVVTRVPGESEKEENLAGDIV
                     QQVSVNSRPFLISAALMLVMAIIPGFPALVFLFLAVCLLGIAWKLQKKRTFGTGNNKD
                     AMGADLSNSQNISPGAEPLILNLSSNIYSSDITQQIEVMRWNFFEESGIPLPKIIVNP
                     VKNNDSAIEFLLYQESIYKDTLIDDTVYFEAGHAEISFEFVQEKLSTNSIVYKTNKTN
                     QQLAHLTGMDVYATTNDKITFLLKKLVLSNAKEFIGVQETRYLMDIMERKYNELVKEL
                     QRQLGLSKIVDILQRLVEENVSIRDLRTIFETLIFWSTKEKDVVILCEYVRIALRRHI
                     LGRYSVSGTLLNVWLIGSDIENELRESIRQTSSGSYLNISPERTEQIIGFLKNIMNPT
                     GNGVILTALDIRRYVKKMIEGSFPSVPVLSFQEVGNNIELKVLGTVNDFRA"
BASE COUNT         1090 a          843 c          663 g          993 t
ORIGIN      
        1 ttgtttttaa tctgatattt atacaacgac actttatcca aattcaccag cgatgcacaa
       61 tatgcctgct tagaggcaat cgactcttta atggattgct cgcagtcatc gagtttacca
      121 tccacctctt ttatctgaga gttaatctta gccattgatt ctctcaatcg gttcgctctg
      181 cttttacgaa tagataaaat tctgtccaac atactcaggc aaccactttg aataggcttt
      241 caatcgtttt ttcgtaacta ctgatatctt tggtgctttg ttgtataaag ttctgaatta
      301 atttcctatt ttttattgcc ttatctgctt caggatcttg ccccatcgtg tactcaccaa
      361 tacgaataag cagctcaaca tttttataag ttgcaataag ctttttgcat tctgccgccg
      421 cacgcaaatg ctccgatgta acaacgttat gcataacacg actggcactt aaaccaatat
      481 caattgcagg gaaatggttt tcctctgcaa gttctcgggt aagtacgatg tgcccatcaa
      541 gaatagagcg gacttcatcg ccaataggat cattaacatt atcgctttct aacaacaccg
      601 tataaatcgc ggttattgaa ccttttggcg caggccccgc tcgctctaat aatttgggta
      661 acgaagaaaa aacactcgga gggaatcccc cccttacatc aggttctccc gatgcaagcc
      721 caacatcacg tgcggctcgg gcataacgag ttacagaatc catcattaat aatacatttt
      781 taccttgatc gcggaagtac tctgcaatag tcgtcgccgt aaatgcggct ttcatccttt
      841 ccagcgcggg gcggtctgac gttgtgacga ccagtacaca tttagaaagc gtggattgag
      901 gtaagagcgc gaggaattca tttacttcgc gaccacgttc gccgataaga gcaaggacaa
      961 taatatctgc ggatgcgcca ttacaaatca tccccaaaag cgtactttta ccaacgcctg
     1021 aaccagcaaa aataccgata cgctgcccaa taccgcaagt tagtagccca tcaattgccc
     1081 tgacgccaag tataaatggc tgatcaataa cctgccttaa taaaggatct ggtggttcag
     1141 catacaagct gcgttcaaac ggaagatagg gggcagcaat attactctcc attggtctgc
     1201 ctatgccatc aacaagccgc ccaagtagtg cgtctccaac acgaatcttg aactcatctc
     1261 cctgataact taaccactga ccacagtaca tgccggaaac atgctcaaaa ggcaacaaga
     1321 aaacctcatc ttcatcaata gcgatcacct cggctaaacg ttgactgggt tctatcttat
     1381 aaaaggcgcc aatacgcgct tttggtaaac gcgccttaat aatcgtcccg ccgatattga
     1441 taatcttgcc ttcatatctg gtagacgaat ttaatgataa tgccggcaca gtgctattga
     1501 gcactttctg aatacgtggg tatttttcca atacagaatc atgctctgaa atcatttacc
     1561 gttcctaata ctttaagttc gatattattc ccaacctcct gaaaagagag cacggggact
     1621 gacgggaacg aaccttcaat cattttcttc acatagcgcc tgatatctaa agcggtcaga
     1681 atgacgccgt tccccgttgg attcatgata ttttttaaga agccaattat ctgctcagtt
     1741 cgctccggtg agatattcag atacgaacct gatgacgttt gtctgatcga ctctcgtagc
     1801 tcattttcaa tatcagagcc aataagccaa acgttcagaa gtgtaccgct aacgctatag
     1861 cgacctaaaa tatgccgacg cagggcaata cgaacgtatt cgcacaagat aaccacatcc
     1921 ttttcttttg ttgaccaaaa aataagcgtc tcaaagatag ttctcaggtc tctaattgag
     1981 acattttcct ctacgagacg ttgtaggatg tcaacaattt tgctcaaacc aagctggcgc
     2041 tgcagctctt tcacaagctc gttatatttt ctctccatga tgtccatcaa ataacgcgtt
     2101 tcttgtacgc cgatgaactc tttggcatta gacaatacaa gtttctttag caaaaacgtt
     2161 atcttatcat ttgttgttgc ataaacatcc atacctgtaa ggtgagcgag ctgttgatta
     2221 gtcttattcg tcttatatac gatagagtta gtcgaaagct tttcctggac aaattcgaat
     2281 gatatctctg catgcccagc ctcaaaatag acagtatcat ctataagagt atctttgtat
     2341 attgactctt gatagagcaa aaattctatt gcgctatcat tatttttaac cggattaaca
     2401 ataatcttag gcaatggaat tccactttcc tcaaagaaat tccaacgcat gacctcaatt
     2461 tgctgtgtaa tgtctgaact ataaatatta ctacttaagt ttaaaatcaa tggctcagcg
     2521 cccggtgaga tgttttggct attagacaaa tcagctccca tagcatcctt attattgcca
     2581 gttccaaatg ttcttttctt ctgtaatttc caggctatcc ccaacaggca aaccgccaga
     2641 aataagaaga ccaatgcagg aaagcccgga ataatcgcca ttacgagcat tagtgcggca
     2701 ctgattaaaa aagggcgact attaacagaa acctgttgaa caatatcacc tgcaaggttt
     2761 tcttcttttt cgctttcacc gggcacacga gtaacaacca cgcctgcggt gacagaaata
     2821 ataagcgcag ggatctgggc aaccaaagca tcaccgacag acaatacaga aaacaggcta
     2881 agtgccgcac taaatggcat gtcaaattgc cacataccaa tgagcacgcc accaaaaagg
     2941 ttaactaaaa caataatgat accagcaata gcgtcccctt ttacaaactt catcgccccg
     3001 tccattgaac cataaaactg gctttccttt tgcactcggg agcgaagaac ttttgcctca
     3061 agcgggtcaa taacgcctgc gcgcatatca ccatcaatac tcatttgctt tcctggcata
     3121 ccatcaaggg aaaaacgagc actcacttca gcaacacgct cggccccttt tgtaataacc
     3181 ataaattgaa caatggtgat aatagtaaaa ataaccaggc caacaacaat attaccgcca
     3241 acaacaaagt taccgaagga gtaaataatg tcacccgcat cgtgatgcag cagaataagt
     3301 ctggttgtac taacactgag cgacaggcgc atcaacgtcg taatcaacag gattgtgggg
     3361 aaagaagtta gttcaagagg attttttata tagattgaga gcattaataa aagtaaagct
     3421 gtcgaaatat taatcgcgat aatgatatcc accacaactg ttggtagtgg aataatcatc
     3481 atcattacag ccataaagaa gaagagagcc agaataagat cgtgatatga ctctgctttt
     3541 ttaaatatat ttaagagttt attcatgatg tcatcctgcg aacgcgctc
//
LOCUS       DAZKIP010000247         3446 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZKIP010000247 DAZKIP010000000
VERSION     DAZKIP010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.249"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..594
                     /locus_tag="ACHOUK_004796"
     CDS             <1..594
                     /locus_tag="ACHOUK_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900054.1"
                     /translation="GGNPPVFSLTSDGKLTAKNADISGSVNANSGTLNNVTVNENCTI
                     KGMLEATQVRGDFVKAVSKSFPKQAGTWGNTETPNGTVTVTISDDHNFDRQIIIPPII
                     FNGIAYSDPGSGNNPGGTRYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGRG
                     SVTLEFKVFHKGNQRAGNITDCTVIVTKKAASGISIR"
     gene            665..1264
                     /locus_tag="ACHOUK_004797"
     CDS             665..1264
                     /locus_tag="ACHOUK_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP9900055.1"
                     /translation="MRKVCAAILSAAICLAVSGVPAWASEHQSTLSAGYLHASTDAPG
                     SDDLNGINVKYRYEFTDTLGLITSFSYANAEDEQKTHYSDTRWHEDYVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYFRVTDNKRKTHDVLTGSDDARYSNTSLAW
                     GAGVQFNPTESVAVDVAYEGSGSGDWRTDGFIVGVGYKF"
     gene            1324..2640
                     /locus_tag="ACHOUK_004798"
     CDS             1324..2640
                     /locus_tag="ACHOUK_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFP9900056.1"
                     /translation="MAAVQISGVLKDGAGKPIQNCTIQLKARRNSTTVVVNTVASENP
                     DEAGRYSMDVEYGQYSVTLLVEGFPPSHAGTISVYEDSQPGTLNDFLGAMTEDDARPE
                     ALRRFEQMVEEAARHAEEAKKNAGEAETSARNAGISASKAEASAANADTSAEDASESA
                     RQAAESAASAKKSEEASSSSASEAAQKASESLQSATDAELSKKTAESAAGNAARDATT
                     ATEKARESAESAQSAEQSRIAAEDAVNRIPTVVGPPGPKGEPGPAGPQGPKGDKGERG
                     DTGPAGATGERGPGGDTGPAGPQGPKGDRGERGETGLTGSTGPQGPKGDTGATGPAGP
                     QGPKGETGAAGPVGATGPQGAKGDPGETQIRFRLGPMRIIETNSYGWFPGTDGALITG
                     LTFLDPKDATQVQGMFQHLQVRFGDGPWQDVKGLDEVGSDTGRTGE"
     gene            2642..2911
                     /locus_tag="ACHOUK_004799"
     CDS             2642..2911
                     /locus_tag="ACHOUK_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFP9900057.1"
                     /translation="MNILRKLMQSLCGCGKHDDCENGRSLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            2913..3020
                     /locus_tag="ACHOUK_004800"
                     /pseudo
     CDS             2913..3020
                     /locus_tag="ACHOUK_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309586.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3088..>3446
                     /gene="nleB2"
                     /locus_tag="ACHOUK_004801"
     CDS             3088..>3446
                     /gene="nleB2"
                     /locus_tag="ACHOUK_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB2"
                     /protein_id="HFP9900058.1"
                     /translation="MLSPIRTTFHNSVNIVQSSPCQTVSFAGKEYELKVIDEKTPILF
                     QWFEPNPERYKKDEVPIVNTKQHPYLDNVTNAARIESDRMIGIFVDGDFSVNQKTAFS
                     KLERDFENVMIIYREDV"
BASE COUNT          952 a          731 c         1040 g          723 t
ORIGIN      
        1 ggtggaaatc caccggtatt ttccctgaca tcagacggaa agctgaccgc taaaaatgcg
       61 gatatcagtg gcagtgtgaa tgcgaactcc gggacgctca acaacgtcac ggtaaatgaa
      121 aactgtacga ttaagggcat gctggaggcg actcaggtca gaggtgactt cgttaaagct
      181 gtatccaaat catttccgaa acaggctggt acgtggggta acacggaaac accaaacggg
      241 acggttacag tcaccatcag cgatgatcat aactttgacc gtcaaatcat tattccgccc
      301 attatcttta acggaatagc gtatagcgat ccgggaagtg gtaataaccc gggaggtaca
      361 agatacacgg gttatggttt tgaagttcgc aaaaacggtg tattaatcgc atccagagaa
      421 actaaagggg ccattcccgg tagctacagt gcggttattg atatgccgag tggcagggga
      481 agcgtcactc tggagtttaa ggttttccat aaaggcaatc agcgggcagg taatatcacc
      541 gactgtacgg tgattgtgac caaaaaagcg gcttccggca tcagtatccg ttgaaatatt
      601 tataacccta ataacgggcg ccagaaatgg cgcctttttt attgcagaaa atcgggaggt
      661 aattatgcgt aaagtttgtg cagcaatttt gtccgcagcc atttgtctgg ccgtatccgg
      721 tgtgcctgca tgggcgtctg agcatcagtc cacgctgagc gcaggatatc ttcatgccag
      781 tacggacgct cccggcagcg atgatctgaa cgggattaac gtgaaatacc gttatgagtt
      841 tacggacaca ctggggctga ttacgtcctt cagttatgcc aacgccgaag atgagcaaaa
      901 aacgcattac agcgataccc gctggcatga ggattacgtg cgtaaccgct ggttcagcgt
      961 gatggcaggg ccgtctgtgc gtgtgaatga atggttcagt gcgtatgcga tggcgggtgt
     1021 ggcttacagc cgtgtgtcga ctttttccgg ggattatttc cgcgtaactg acaacaagag
     1081 gaaaacgcac gacgtgctga ccggaagtga tgacgctcgc tacagcaaca catctctggc
     1141 gtggggggct ggcgtgcagt ttaacccgac cgaatcagtg gccgttgatg tcgcttatga
     1201 aggctccggc agcggtgact ggcgcactga cggtttcatc gtgggtgtcg gttataaatt
     1261 ttgattagta acacagcgtt atgacagccc gccggttcag gcgggctttt ttgtggagtg
     1321 ggtatggcag cagtacaaat atcaggcgtg ctgaaagatg gtgcgggaaa accaatacag
     1381 aactgcacca ttcaactgaa agccagacgt aacagcacca cggtggtggt gaacacggtg
     1441 gcctctgaaa atccggatga ggcagggcgt tacagcatgg acgtcgagta tggtcagtac
     1501 agcgtcactc tgttggtgga gggattcccg ccatcacatg ccgggaccat ctccgtgtat
     1561 gaagattctc aaccgggtac gctgaatgat tttctcggtg ccatgacgga ggatgatgcc
     1621 cgtccggagg cactgcgtcg ttttgaacag atggtggaag aagcagcgcg tcacgcagag
     1681 gaggcgaaga agaatgccgg agaagcagag acgtccgcga ggaatgccgg catatcagcc
     1741 agtaaggcgg aagcgagcgc cgcaaatgct gatacttcag cagaggatgc atcggagtca
     1801 gcccggcagg cggcagaaag tgcagcctct gcaaagaagt cagaggaagc gtcctcgtcc
     1861 tcagcctctg aggccgctca aaaagccagt gagtcattac aaagtgcaac agatgccgag
     1921 ttgtcaaaaa agacggcaga aagtgcagcc ggtaatgcag ccagggatgc aacgaccgca
     1981 acagaaaaag cccgggaatc agcagaaagc gcacagtcag cggaacaaag cagaatagcg
     2041 gcggaagacg ccgtaaacag aattcccacc gtggtggggc ctcccggacc aaagggggaa
     2101 ccgggtcccg cgggtcctca ggggccgaag ggagataaag gagagcgtgg agacaccggt
     2161 ccggcagggg caaccggtga acggggaccg ggaggagata caggtccggc aggtccgcag
     2221 gggccgaaag gcgacagggg agagcgggga gagaccggtc tgacaggaag tacaggtcca
     2281 caggggccaa agggagatac cggggcaaca ggtccggcag gaccgcaggg accgaaaggg
     2341 gaaacaggtg cggctggccc ggtgggggct accggacctc agggggcgaa gggcgacccg
     2401 ggggagacac aaatacggtt ccgtctgggg ccgatgagaa ttattgagac aaacagctat
     2461 ggctggttcc cgggtacaga tggtgcgctc atcaccggac tgacctttct tgaccccaaa
     2521 gatgccacac aggttcaggg gatgtttcag catttgcagg tcagatttgg tgacgggcca
     2581 tggcaggatg ttaagggact ggatgaagtg ggcagtgata caggcagaac tggagaatga
     2641 catgaatatt ttgagaaagc ttatgcagag tctgtgcggt tgcggaaagc atgatgactg
     2701 tgaaaacgga cggtcactta cagcacaatt gcgactggga ccggcagaca ttctggagtc
     2761 agatgagaat ggcattattc cggagcagga cagggtaatc acacaggtgg tgatactgga
     2821 tgcagataaa aagcagatac agtgtgtggt aagaccgctg caaatcctgc gtgctgacgg
     2881 gacgtgggaa aatattggcg ggatgaagta acccgacagc ttcacaaaac cggagtccgg
     2941 ctccggtttt tgttgtcatg tccggtggat gtttgttagg aaagcaaaga tggcaaaact
     3001 gctggaggtt ttgtggttga gtatgccaat ataattaata gattaaagag ttagttgtga
     3061 agaaaatatg gataaacagg acgacgaatg ctttcaccga taaggacaac tttccataac
     3121 tcagtaaata tagtgcagag ttcaccctgt caaacggttt cttttgcagg aaaggaatat
     3181 gagttaaagg tcattgatga aaaaacgcct attctttttc agtggtttga acctaatcct
     3241 gaacgatata agaaagatga ggttccaata gttaatacta agcagcatcc ctatttagat
     3301 aatgtcacaa atgcggcaag gatagagagt gatcgtatga taggtatttt tgttgatggc
     3361 gatttttcag tcaaccaaaa gactgctttt tcaaaattgg aacgagattt tgaaaatgta
     3421 atgataatct atcgggaaga tgttga
//
LOCUS       DAZKIP010000248         3415 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZKIP010000248 DAZKIP010000000
VERSION     DAZKIP010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.250"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..71
                     /locus_tag="ACHOUK_004802"
     CDS             <1..71
                     /locus_tag="ACHOUK_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900059.1"
                     /translation="HGSQVDLIFRFLDHAFATGVLG"
     gene            84..443
                     /locus_tag="ACHOUK_004803"
     CDS             84..443
                     /locus_tag="ACHOUK_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP9900060.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVVLIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYKIESE"
     gene            452..982
                     /locus_tag="ACHOUK_004804"
     CDS             452..982
                     /locus_tag="ACHOUK_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFP9900061.1"
                     /translation="MIYPEITGKSGEHLRLNTLEAVWIQGKLRMWGRWSYIGGGKSGN
                     MFNRLLVSKKLTKTAVNEVLRSMKKSGLEKPELEAFFRDMTRGKQKSWLSHCTDTEAL
                     IIDRVISEVLGEYPGLINILRQRYEGRGMSKRKMAECLNRTHPEWCFSTCEKRIAGWL
                     AVAEHMLYVPMHDSFR"
     gene            1224..1421
                     /locus_tag="ACHOUK_004805"
     CDS             1224..1421
                     /locus_tag="ACHOUK_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFP9900062.1"
                     /translation="MLKQQDMTETARVVFNELSVTDPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            1572..2630
                     /locus_tag="ACHOUK_004806"
     CDS             1572..2630
                     /locus_tag="ACHOUK_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFP9900063.1"
                     /translation="MLNTVKISSCELINADCLEFMRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWAGDEDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDTEIMMRERFNVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRTLKQHVMA
                     PLIAYFRDARAVLGITAKQIADATGKKNMVSHWFSAGQWQLPNESDYLKLQALFARVA
                     EEKHQRGELEKPHHQLVDTYASLNRQYAELQSEYKHLRRYFGVTVQVPYTDVWTYKPV
                     QYYPGKHPCEKPAEMLQQIISASSRSGDLVADFFMGSGSTVKAAMALGRRATGVELET
                     ERFEQTVREVQDLIIRNG"
     gene            2671..2746
                     /locus_tag="ACHOUK_004807"
     tRNA            2671..2746
                     /locus_tag="ACHOUK_004807"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2704..2706,aa:Ile,seq:cat)
     gene            2827..2903
                     /locus_tag="ACHOUK_004808"
     tRNA            2827..2903
                     /locus_tag="ACHOUK_004808"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2861..2863,aa:Arg,seq:tct)
BASE COUNT          869 a          741 c          965 g          840 t
ORIGIN      
        1 agcatggttc ccaggttgat ttaatttttc gttttcttga tcacgccttt gcgactggcg
       61 tgctcgggta aaagaggtga ctgatgctca tagatttggt tttaccttac ccgccgacgg
      121 tgaacactta ctggcgacgc cgtggcagca catattttgt atcaaaagcc ggggagcgtt
      181 atcgccgggc agtggtgctt attgttcgcc agcagcgact gaaattaagc ctgtccggac
      241 ggctggcaat aaaaattatt gcagagccac cggataagcg tcgtcgtgac ctggacaata
      301 ttctgaaagc accgctggat gcgctgacgc atgcgggagt gttaatggac gatgagcagt
      361 ttgatgaaat caatattgta cgtggtcagc cagtatctgg tggacggctg ggtgtgaaga
      421 tttacaaaat tgagagtgag tgagcgtaaa tatgatatat ccggaaatta caggcaaaag
      481 cggcgaacat ttacgcctga acacgctgga agcagtctgg atccagggga aattacggat
      541 gtgggggcgg tggtcgtata tcggtggggg taaatccgga aatatgttta accggttact
      601 ggtttcgaaa aagctgacga aaacagcagt taatgaggtt ttacgcagca tgaagaaatc
      661 cgggctggaa aaaccggaac ttgaggcatt ttttcgggat atgaccagag ggaagcagaa
      721 gagctggttg tcacattgta cagacacaga ggcgttgatt attgatcgcg ttatcagtga
      781 ggtgcttggg gaatatcccg ggctaatcaa tattctccgg caaaggtacg aaggacgggg
      841 aatgagtaag agaaaaatgg cagaatgttt aaatcgtact cacccggaat ggtgtttcag
      901 cacatgtgag aaacgtattg caggttggtt agccgtggct gaacacatgc tttatgtacc
      961 tatgcacgat tcatttcgat aaaaaaagct tgctttttta cgcagaaaca gcttgaattc
     1021 ctgtaagctt cgcaaagctg tatcgcgagg cgaaatgcaa gttttttcgc acaaggaagc
     1081 caccggaagg tggttttttt gtgtccgtaa tatacagcag cgcaataaat tcgctggtgg
     1141 ttattaatac cgttctttca ggttgctggc tttttcgaca agagttattg gtgtgtcacg
     1201 ttaaccggaa aagggaaaaa gacatgctga aacagcagga tatgacagaa accgccagag
     1261 tggtgtttaa tgaattaagc gttaccgacc cggcgacagt cggggagatt gcgcagaata
     1321 cttacctttc acgcgaacgc tgccagttaa tactgaccca gctggttatg gcgggtctgg
     1381 cagactatca gtttggttgt tacagacgcc ttcagtcctg aaggcttttt tatttgtggt
     1441 aaatgggcgg ctggtgggtg taaggggcac ccaccagcca tctgctcatg cgttgggttc
     1501 acaagcaaac ctcaggccca ctgctttgcg caaaagcaga atgagcctat cagagacagg
     1561 cttaatgatc catgcttaat actgtaaaaa tatccagttg tgagttaatc aacgccgact
     1621 gcctggaatt tatgcggtcg ttacccgaaa attctgttga cctgatagtc acggacccgc
     1681 cgtacttcaa agtgaaaccc gagggctggg ataaccagtg ggcgggtgat gaagattacc
     1741 tgaagtggct ggaccagtgt cttgcgcagt tctggcgggt gctgaaacct gccggaagtc
     1801 tttacctgtt ctgtggccat cgtctggcat ctgacaccga aatcatgatg cgtgagcggt
     1861 ttaacgtgct gaaccatatc atctgggcaa agccgtccgg acgctggaac gggtgcaaca
     1921 aggaaagcct gcgggcgtat ttccccgcca cagagcgcat tctgttcgca gagcattatc
     1981 aggggccgta tcgtccgaaa gatgccgggt atgaggcgaa gggtaggaca ctgaaacagc
     2041 atgtgatggc cccgctgatt gcttactttc gtgatgcgcg cgctgtcctg gggataacgg
     2101 caaaacagat tgcagatgcc acaggaaaga aaaacatggt gtcgcactgg ttcagtgccg
     2161 gtcagtggca gctgccgaac gaaagcgatt atctgaaatt acaggcactg tttgcccggg
     2221 tggcagaaga gaagcatcag cggggtgaac tggaaaagcc ccaccaccag ctggtggata
     2281 cgtatgcctc tctgaaccga cagtatgcgg agctgcagag tgaatataag catctgcggc
     2341 ggtatttcgg tgtgacggtg caggtgccgt acaccgatgt gtggacgtat aaaccggtgc
     2401 agtactatcc agggaaacat ccgtgcgaaa aaccggcaga aatgttgcag cagataatca
     2461 gcgcaagcag tcgttcggga gacctggttg cagatttctt catggggtcg gggtcgacag
     2521 tgaaagcagc gatggcgctg ggacgtcgtg caactggcgt tgaactggag actgaacgtt
     2581 ttgagcagac ggtgcgggaa gtacaggatt taatcattcg taacggatga gattgcggag
     2641 ttaatcatgc gtcgttatta ttcagcaatc ggccctttag ctcagcggtg agagcgagcg
     2701 actcataatc gccaggtcgc tggttcaaat ccagcaaggg ccaccaaccg ccactagctc
     2761 atcaggaaag aacgtcaccc tgtgcgagat tcggagtccc cggtggcggt ccattatcgg
     2821 tattctgcgt tgttagctca gccggacaga gcaattgcct tctaagcaat cggtcactgg
     2881 ttcgaatcca gtacaacgca ccacaccaca cttatctgcc ctgactctct tttgcgggct
     2941 ttttattaca ggaaagacac cggacagtga aatgttaaat gcctcacaat tcaggcagtt
     3001 gactgttgcc tgacatgctg agcgtttgtt aaaaaaatcc tgcatgatga atccccctga
     3061 gcggcggggc ataatgacag atgtttggtt gcgtattgta taggcaagtt gcggattctg
     3121 tctggtcatt gcagaattca ccgggaggca cccggcatca tgctgtatac agagattagg
     3181 catatatcca ggcttctcat cgcaggagcc tttttacatg caaaaaaaag cccgagtggg
     3241 ttcgggcaac agcatgagat acttgcattg tcatttttat cgtgtggatt ttaaccaggg
     3301 tttataaggc tgcgcaactg cgcggccttt ttcgttttgc gggctgcggt tctcctcttt
     3361 tgattctcct tgtggccgga ccgtggcccg caactgttga ggaaaatccc ggaaa
//
LOCUS       DAZKIP010000249         3368 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZKIP010000249 DAZKIP010000000
VERSION     DAZKIP010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.251"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            64..642
                     /gene="espA"
                     /locus_tag="ACHOUK_004809"
     CDS             64..642
                     /gene="espA"
                     /locus_tag="ACHOUK_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     filament protein EspA"
                     /protein_id="HFP9900064.1"
                     /translation="MDTSNATSVVNVSASSSTSTIYDLGNMSKDEVVKLFEELGVFQA
                     AILMFSYMYQAQSNLSIAKFADMNEASKASTTAQKMANLVDAKIADVQSSTDKNAKAK
                     LPQDVIDYINDPRNDISVTGIRDLSGDLSAGDLQTVKAAISAKANNLTTVVNNSQLEI
                     QQMSNTLNLLTSARSDVQSLQYRTISAISLGK"
     gene            655..1779
                     /gene="espD"
                     /locus_tag="ACHOUK_004810"
     CDS             655..1779
                     /gene="espD"
                     /locus_tag="ACHOUK_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     pore-forming subunit EspD"
                     /protein_id="HFP9900065.1"
                     /translation="MLNVNNDTLSVTSGVNTASGTSGITQSETGLSLDLQLVKSMNSS
                     AGWTESSPLPTPPAGHSLVTPSAAEDVLSKLFGGISGEVTSRTEEAEPQRTSYPYLSQ
                     VNTVDPQQMMMMVTLLSLDTSAQKVSSLKNSNEIYMDGQTKALENKTQEYKKQLEEQQ
                     KAEEKSQKSKIVGQVFGWLGVALTAVAAVFNPALWAVVAIGATAMALQTAVDVMGENA
                     PQGLKTAAQVFGGISMAASILTAGVGGVSSLLSKFGNVANKIGSSVVKVVEKAAEALV
                     KNVFAKISTVAEGVTNGIRSAGTTALNNEAAQLQMLSQLAAFAVQNLTRQSESLGESA
                     KLELDKAASELQNQASYLQSVSQLMSDSARVNSRIVSGRI"
     gene            1800..2738
                     /gene="espB"
                     /locus_tag="ACHOUK_004811"
     CDS             1800..2738
                     /gene="espB"
                     /locus_tag="ACHOUK_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     pore-forming subunit EspB"
                     /protein_id="HFP9900066.1"
                     /translation="MNTIDNTQVTMVNSASESTTGASSAVAASALSIDSSLLTDGKVD
                     ICKLMLEIQKLLGKMVTLLQDYQQKQLAQSYQIQQAVFESQNKAIEEKKAAATAALVG
                     GIISSALGILGSFAAMNNAAKGAGEIAEKASSASSKAAGAASEVANKALVKATESVAD
                     VAEEASSAMQKAMATTTKAASRASGVADDVAKASDFAEDLADAAEKTSRINKLLNSVD
                     KLTNTTAFVAVTSLAEGTKTLPTTISESVKSTHEVNEQRAKSLENFQQGNLELYKQDV
                     RRTQDDITTRLRDITSAVRDLLEVQNRMGQSGRLAG"
     gene            2745..3152
                     /gene="cesD2"
                     /locus_tag="ACHOUK_004812"
     CDS             2745..3152
                     /gene="cesD2"
                     /locus_tag="ACHOUK_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LcrR family type III secretion system chaperone
                     CesD2"
                     /protein_id="HFP9900067.1"
                     /translation="MVDTFNDEVFNYYLEQKGYTIQKEFLCGSAFFIGWRIETPFFSL
                     AYRLDEQELILCSFEARNQTGLNGPVLSLTHLLEELYHHFSGIKKISAMKSKIGSDSE
                     RQKREELFNYFIRKGAVQQETEDGIWFVMNVNS"
     gene            3188..>3368
                     /gene="escF"
                     /locus_tag="ACHOUK_004813"
     CDS             3188..>3368
                     /gene="escF"
                     /locus_tag="ACHOUK_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE needle filament
                     protein EscF"
                     /protein_id="HFP9900068.1"
                     /translation="MNLSEITQQMGEVGKTLSDSVPELLNSTDLVNDPEKMLELQFAV
                     QQYSAYVNVESGMLKT"
BASE COUNT          990 a          640 c          824 g          914 t
ORIGIN      
        1 tttgatatta attactcaat aatttttttg ttttcctgag aaaaattatc aagaggtata
       61 tagatggata catcaaatgc aacatccgtt gttaatgtga gtgcgagttc ttcgacatcg
      121 acgatctatg acttaggtaa tatgtcgaag gatgaggtgg ttaagctatt tgaggaactc
      181 ggtgtttttc aggctgcgat tctcatgttt tcttatatgt atcaggcaca aagtaatctg
      241 tcgattgcaa agtttgctga tatgaatgag gcatctaaag cgtcaaccac ggcacaaaag
      301 atggctaatc ttgtggatgc caaaattgct gatgttcaga gtagcactga taagaatgcg
      361 aaagccaaac ttcctcaaga cgtgattgac tatataaacg atccacgtaa tgacataagt
      421 gtaactggta ttcgtgatct tagtggtgat ttaagcgctg gtgatctgca aacagtgaag
      481 gcggctattt cagctaaagc gaataacctg acaacggtag tgaataatag ccagctcgaa
      541 attcagcaaa tgtcgaatac attaaatctc ttaacgagtg cacgttctga tgtgcaatct
      601 ctacaatata gaactatttc agcaatatcc cttggtaaat aaccggagat aactatgctt
      661 aacgtaaata acgataccct gtctgtaacg tctggggtta ataccgcctc gggtacttct
      721 ggtattactc aatctgaaac gggtttatcg ctggatttac aactggttaa atccatgaac
      781 tcgtcagcag gctggacaga aagtagccct ttaccgacgc cgccggcagg tcactcatta
      841 gtgacgccct ctgctgctga ggatgtcctt agtaaattgt ttggtggtat tagtggtgag
      901 gttacaagtc gcactgagga ggcagagcca cagcgcacaa gctatcccta tctctctcag
      961 gtgaataccg ttgaccctca gcaaatgatg atgatggtca ctctgttatc cctggatact
     1021 tccgcgcaga aagtctcgag tctgaaaaac tctaacgaga tttatatgga tgggcaaact
     1081 aaagcgctgg agaataaaac gcaggagtat aaaaaacagc tcgaagaaca acagaaagcc
     1141 gaagagaaat cacaaaaaag taaaattgtt ggccaggtct ttggttggtt gggcgtcgca
     1201 ttaacagccg ttgccgctgt ttttaaccca gcactctggg ctgttgttgc cattggtgca
     1261 acagcaatgg cactgcaaac ggcagtcgat gtaatggggg aaaatgcccc tcagggatta
     1321 aagactgcag cacaggtctt tggcggaata tctatggccg caagcattct gacagccggc
     1381 gttggcgggg tgtcttcact gttatctaaa tttggtaatg ttgctaacaa aattggctca
     1441 agcgttgtaa aagtcgttga gaaggcggca gaagcgctgg ttaaaaacgt ttttgcaaaa
     1501 atttcgacag tggctgaggg cgttacgaac ggtattcgtt ctgccgggac aactgcgttg
     1561 aataatgagg ctgcgcaact ccaaatgttg tctcagttag ctgctttcgc ggtgcaaaac
     1621 ttaactcgac agagtgaaag cttaggtgag agtgcgaagc tcgagctgga taaagcggca
     1681 agcgagttac aaaatcaggc gagctattta caaagtgttt ctcaactgat gtccgattca
     1741 gcacgggtaa atagtcgtat tgttagtggc cgaatttaat tattaaagag aattttatta
     1801 tgaatactat tgataatact caagtaacga tggttaattc cgcttcggag agtacgaccg
     1861 gcgcttccag tgcagttgcc gcatctgctt tatcaattga ttcatctctg cttactgatg
     1921 gtaaggttga tatttgtaag ctgatgctgg aaattcaaaa actcctcggc aagatggtga
     1981 ctctattgca ggattaccaa caaaaacaat tggcgcaaag ctatcagatt cagcaggccg
     2041 tttttgagag ccagaataaa gctattgagg aaaaaaaagc cgcggcaacc gctgctttgg
     2101 ttggcgggat tatttcatca gcattgggga tcttaggttc ttttgcagca atgaacaacg
     2161 cggctaaagg ggctggtgag attgctgaaa aagcaagctc tgcatcttca aaggctgctg
     2221 gtgcggcttc tgaggttgca aataaagctc tggtcaaggc tacggaaagt gttgctgatg
     2281 tcgcagagga ggcatccagt gcgatgcaga aagcgatggc cacaacaacg aaagcagcca
     2341 gccgtgcatc tggcgttgca gatgatgttg cgaaagcctc tgactttgct gaagatcttg
     2401 cagacgccgc cgagaagaca agcagaatca ataagttgtt gaattccgta gataaactga
     2461 ccaataccac agcatttgtt gccgtgacca gtcttgctga aggtacgaaa acgttgccaa
     2521 caacaatatc tgagtccgtc aaatcgactc atgaggttaa tgaacaacgt gcgaagtcgc
     2581 tggaaaactt ccagcagggg aatctggagc tgtataaaca agacgttcgc agaacgcagg
     2641 atgatatcac gactcgtctg cgtgatataa cgtccgctgt ccgcgatctc cttgaggtcc
     2701 agaatcgtat ggggcaatcg ggtcgcttag ctgggtaatt gatcatggtc gatacgttta
     2761 atgatgaagt gtttaattac tatcttgaac aaaaagggta cacaatacag aaggagtttc
     2821 tttgtggcag tgcctttttt atcggatggc ggattgagac accttttttt tcattagcgt
     2881 acagactgga tgaacaagaa ctgattttgt gctcttttga agcacgtaac caaacagggc
     2941 ttaacggccc tgttttatcg ctgactcatt tgctcgaaga gttgtatcac catttttcgg
     3001 gtattaagaa aatcagcgcg atgaaatcta agattggttc agattcagaa cgtcaaaagc
     3061 gcgaagagtt gtttaattac ttcatcagaa agggtgccgt gcagcaagaa acagaagacg
     3121 gaatttggtt cgtaatgaac gtaaatagtt aaaagttttt attttattaa cttctgaggg
     3181 aaatttaatg aatttatctg aaattactca acaaatgggt gaagtaggta aaacgctgag
     3241 cgattctgtg ccagagttac ttaatagcac cgatttggtt aatgaccctg aaaaaatgtt
     3301 agagttgcag tttgcggttc agcaatattc tgcttatgtt aacgtagaaa gtggaatgtt
     3361 gaaaacga
//
LOCUS       DAZKIP010000250         3301 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZKIP010000250 DAZKIP010000000
VERSION     DAZKIP010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.252"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            224..1156
                     /gene="rfaD"
                     /locus_tag="ACHOUK_004814"
     CDS             224..1156
                     /gene="rfaD"
                     /locus_tag="ACHOUK_004814"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFP9900069.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LNIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            1166..2212
                     /gene="rfaF"
                     /locus_tag="ACHOUK_004815"
     CDS             1166..2212
                     /gene="rfaF"
                     /locus_tag="ACHOUK_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFP9900070.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLKELNALLLQEEA"
     gene            2216..3208
                     /gene="rfaC"
                     /locus_tag="ACHOUK_004816"
     CDS             2216..3208
                     /gene="rfaC"
                     /locus_tag="ACHOUK_004816"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFP9900071.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPEIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGENQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            3205..>3301
                     /locus_tag="ACHOUK_004817"
     CDS             3205..>3301
                     /locus_tag="ACHOUK_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900072.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFL"
BASE COUNT          832 a          807 c          865 g          797 t
ORIGIN      
        1 tgcttccgtt atttatcata catcggaata ttgatactaa agcactattc acatgcaaaa
       61 ccaacatccg ccatgaagga ctagctaaaa cccaaactag tttgttgcaa ttagcatcct
      121 tgcacctcta tgtaaagggc tgaagggatt cggatgtgat ggtatgatta cagacattcg
      181 tgtctgagat tgtctctgac tccataattc gaaggttaca gttatgatca tcgttaccgg
      241 cggcgcgggc tttatcggca gcaacatcgt taaagccctg aatgataaag gcatcaccga
      301 tattctggtg gtggacaacc tgaaagacgg caccaagttt gtgaacctgg tggatctgaa
      361 tatcgcagac tatatggata aggaagactt cctgatccag attatggctg gcgaagagtt
      421 cggcgatgtc gaagcgattt tccacgaagg cgcgtgctct tccaccaccg agtgggacgg
      481 caagtatatg atggataaca actatcaata ctccaaggag ctgctgcact actgcctgga
      541 gcgtgaaatc ccgttcctgt acgcttcttc cgcagccacc tacggcggac gcacctccga
      601 ctttattgaa tcccgcgagt acgaaaaacc gttgaacgtc tacggttact caaaattcct
      661 gtttgatgaa tatgttcgcc aaatcctgcc ggaagcgaac tcgcagattg ttggcttccg
      721 ctatttcaac gtttatggac cgcgtgaagg ccataaaggc agcatggcga gcgtcgcttt
      781 ccatctcaac acccagctta ataacggtga atcaccgaag ctgttcgaag gtagcgagaa
      841 ctttaaacgc gacttcgtct atgttggcga tgtggcagat gtaaacctgt ggttcctgga
      901 aaatggcgtt tccggcatct tcaacctcgg caccggtcgt gcggaatcct tccaggctgt
      961 agctgatgct acgctggctt atcacaagaa aggccagatc gaatacattc cgttcccgga
     1021 taagctgaaa ggccgctacc aggcgttcac tcaggcagat ctgacaaatc tgcgcgcggc
     1081 gggttacgac aaaccgttca aaaccgttgc tgaaggtgta acggaataca tggcctggct
     1141 gaatcgcgac gcataagagc tctgcatgaa aatactggtg atcggcccgt cttgggttgg
     1201 cgacatgatg atgtcgcaaa gtctctatcg cacgctccag gcgcgctatc cccaggcgat
     1261 aatcgatgtg atggcaccgg catggtgccg tccattatta tcgcggatgc cggaagttaa
     1321 cgaagctatt cctatgcctc tcggtcacgg agcgctggaa atcggcgaac gccgcaaact
     1381 gggtcatagt ctgcgtgaaa agcgctacga ccgcgcctac gtcttaccta actccttcaa
     1441 atccgcatta gtgcctttct tcgcgggtat tcctcatcgc accggctggc gcggcgagat
     1501 gcgctacggt ttactcaacg atgtacgcgt gctcgataaa gaagcctggc cgctaatggt
     1561 ggaacgctat gtcgcgctgg cctatgacaa aggcattatg cgcacagcac aagatctgcc
     1621 gcagccattg ttatggccgc agttgcaggt gagcgaaggt gaaaaatcat atacctgtaa
     1681 tcaattttcg ctttcatcag aacgtccgat gattggtttt tgcccgggtg cggagtttgg
     1741 tccggcaaaa cgctggccac actaccacta tgcggagctg gcaaagcagc tgattgatga
     1801 aggttatcag gtggttctgt ttggctctgc gaaagatcat gaagcaggca atgagattct
     1861 tgccgctttg aataccgagc agcaggcatg gtgtcggaac ctggcggggg aaacacagct
     1921 tgatcaagcg gttatcctga ttgcagcctg taaagccatt gtcactaacg attctggcct
     1981 gatgcacgtt gcggcggcgc tcaatcgtcc gctggttgcc ctgtatggtc cgagtagccc
     2041 ggacttcaca ccgccgctat cccataaagc gcgcgtgatc cgcctgatta ccggctatca
     2101 caaagtgcgt aaaggtgacg ctgcggaggg ttatcaccag agcttgatcg acattactcc
     2161 ccagcgcgta ctgaaagaac tcaacgcgct attgttacaa gaggaagcct gacggatgcg
     2221 ggttttgatc gttaaaacat cgtcgatggg cgatgttctc catacgttgc ccgcactcac
     2281 tgatgcccag caggcaatcc cagaaattaa gtttgactgg gtggtggaag aagggttcgc
     2341 acagattcct tcctggcacg ctgccgttga gcgagttatt cctgtggcaa tacgtcgctg
     2401 gcgtaaagcc tggttctcgg cccctataaa agctgaacgc aaagcgtttc gtgaagcgct
     2461 acaagcagag aactatgacg cagttatcga cgctcagggg ctggtaaaaa gcgcggcgct
     2521 ggttacgcgt ctggcacatg gcgtaaagca tggcatggac tggcaaaccg ctcgcgaacc
     2581 tttagccagc ctgttttaca atcgtaagca tcatattgca aaacagcagc acgccgtaga
     2641 acgcacccgc gaactgtttg ccaaaagttt gggctatagc aaaccgcaaa cccagggcga
     2701 ttatgctatc gcacagcatt ttctgacgaa cctgcctaca gatgctggcg aatatgccgt
     2761 atttcttcat gcgacgaccc gtgatgataa acactggccg gaagaacact ggcgagaatt
     2821 gattggttta ctggctgatt caggaatacg gattaaactt ccgtggggcg cgccgcatga
     2881 ggaagaacgg gcgaaacgac tggcggaagg atttgcttat gttgaagtat tgccgaagat
     2941 gagtctggaa ggcgttgccc gcgtgctggc cggggctaaa tttgtagtgt cggtggatac
     3001 ggggttaagc catttaacgg cggcactgga tagacccaat atcacggttt atggaccaac
     3061 cgatccggga ttaattggtg ggtatgggga gaatcaagta gagtgtcgtt ctacaagtat
     3121 gtctcttgca gatttgccag ctcaaaccgt ttttcaaaat ttaaatcttg aaataataac
     3181 caataagttg acatcggaga taagatgacc tcaacattat ttttctctct cgagaaaaaa
     3241 aactggatag cgtactggaa cagagctctc gtattcttat tcattaccac ctattttttg
     3301 g
//
LOCUS       DAZKIP010000251         3221 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZKIP010000251 DAZKIP010000000
VERSION     DAZKIP010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.253"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            346..552
                     /locus_tag="ACHOUK_004818"
     CDS             346..552
                     /locus_tag="ACHOUK_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP9900073.1"
                     /translation="MEKITTGVSYTTSAVGTEYWLLQLLDKVSPSQWVAIGVLGSLLF
                     GLLTYLTNLYFKIREDRRKVARGE"
     gene            complement(808..1080)
                     /locus_tag="ACHOUK_004819"
     CDS             complement(808..1080)
                     /locus_tag="ACHOUK_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900074.1"
                     /translation="MTENVKSELLLLMADNNEATSSILADPYGKISHKTLDIITTTLT
                     PLMLQRLKHNINAWVNEELSPPCLWDSRYACQQKMRIFNLLSPKLR"
     gene            1240..1773
                     /gene="rrrQ"
                     /locus_tag="ACHOUK_004820"
     CDS             1240..1773
                     /gene="rrrQ"
                     /locus_tag="ACHOUK_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP9900075.1"
                     /translation="MNEKIKYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWDIDR"
     gene            1994..2107
                     /locus_tag="ACHOUK_004821"
     CDS             1994..2107
                     /locus_tag="ACHOUK_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900076.1"
                     /translation="MKGIEVETPASLDLTRAAAFAIRIVAIAVLVWAIRWW"
     gene            2329..2514
                     /locus_tag="ACHOUK_004822"
     CDS             2329..2514
                     /locus_tag="ACHOUK_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFP9900077.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWIMQPA
                     PDWQTPLNGIISSSENG"
     gene            3040..>3221
                     /locus_tag="ACHOUK_004823"
     CDS             3040..>3221
                     /locus_tag="ACHOUK_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP9900078.1"
                     /translation="MLHDHLAECLEKKGLYRRAAERWAKVMVQLSDDQKRKVAAQKRA
                     ECLRKARRTPVSPVNLT"
BASE COUNT          857 a          613 c          865 g          886 t
ORIGIN      
        1 gaacagaacc cgcagaacaa caaccagtta cagatgcttt attaaggaaa aaaacagcca
       61 gcactgactt tcggtggaga ggtgctggct cagaaggata gttggatttc acatgatact
      121 tatgcctggc ggtatatttt ctgacagaca gtgacgggtg ttgtcaagat attgtgtcat
      181 ttataacctg aatcagggga ggccggaatg ttatctggca tttttagcag agcctgaatg
      241 ccataatcac ggctcccgga gttggccgtc agtgggtgac actggcggct tttttgtttt
      301 tctttacttt cattttctgt cggcggtgac ggagacatac atcagatgga aaaaatcaca
      361 acaggtgtgt catacaccac gtcagcggtg gggacggaat actggttact gcagctgctg
      421 gacaaagtct ctccgtccca gtgggtggca ataggtgtgc tgggaagtct gctgtttggc
      481 ctgctgacgt atctgactaa cctgtatttc aaaatcagag aggaccgtcg taaggtggcg
      541 cggggagagt agtcgatgaa taaacaatac gaactggttg taaaatgaat atttctaact
      601 gaaaaaacgt tccatgaggt aagaaaaggt cacaggcaat caataacagg acgtgatgaa
      661 agacccttgc atttgtgcgc tttctcttta gatagcagca gatactgaaa atctgagttg
      721 tcggggagtc agggatacag ctgtgcaaga gttggtcatt gtgattccat tgaaatcctg
      781 tatgccatga agggcaggat tttatggcta cctgagcttt ggtgatagta agttgaaaat
      841 tcgcattttt tgctgacatg cgtaacgaga atcccataag cagggaggac ttaattcttc
      901 attaacccat gcgttgatat tatgtttcag ccgttgaagc atcagcggtg ttaatgttgt
      961 ggtaataata tccagcgttt tatgtgagat cttaccgtaa gggtctgcaa gaatgctgct
     1021 tgttgcttcg ttattatctg ccatcagaag aagtaactct gatttaacgt tttctgtcat
     1081 tagttgtaaa aatcttctgc gcaaactttc tttactgttc atttatatgg cttcatttgt
     1141 tgtaatctgc tgcgtctcaa gggatatgtt tatgagagcg accatgagtg ttggattata
     1201 tacctaacat atcaagggat tagaaatcga taaatcccca tgaacgaaaa aataaaatac
     1261 ggcctgtcgg ctgccgttct ggcgctgatt ggtgcaggtg cttctgcgcc tgaaatcctc
     1321 gaccagtttc tggatgaaaa ggaaggtaac cacaccacgg cataccgtga tggtgcgggg
     1381 atctggacca tctgccgtgg tgccattctg gtggatggta agcctgttat tcctggcatg
     1441 aagctgtcaa aggaaaaatg cgaccgggtt aatgccatcg aacgtgacaa ggcgctggca
     1501 tgggtggaga aaaacatccg ggtgccgctg accgaacccc agaaagcggg gatcgcgtca
     1561 ttctgtccgt acaacattgg ccccggtaag tgcttcccgt cgacgtttta taaacgaatt
     1621 aatgcaggcg atcgaaaagg tgcctgtgag gcgattcgct ggtggattaa ggacggtggc
     1681 agagactgcc gtatccgttc aaataattgc tacggtcagg tctcacggcg tgaccaggag
     1741 agcgcgctgg cgtgctggga catcgacaga tagcagaata ttttcctgaa aaatgacgtt
     1801 ggccaacgcg ggtggataac acgaaatcct gaaaactggt aaaacctaag tgaataaaag
     1861 taaaaacccc gtttgttggc agcaagcggg gttttgtgtt ttctgacctt gagtaaggca
     1921 agggagaaat tatgggtagg gaggtacttt ccctgtgagg aagtataaaa gattctttct
     1981 gaggttgtcc attatgaaag gcattgaagt ggagacgcca gccagtctgg atttaacaag
     2041 agcggcagct tttgccattc gtattgtggc cattgctgtt ctggtctggg caatccgttg
     2101 gtggtgatat gaaccgtgtt ctgtgtgtgg ttatcattgt cctgctggtg gcctgtggtg
     2161 cgcttagtct ggggctgaat cattaccgtg atcacgccat catctacaaa gagcagcgcg
     2221 ataaaaaagc cagtgagctg gagctggcga acgcgacaat tactgatatg cagatacgcc
     2281 agcgtgatgt cgctgcactt gatgccagat actcgaggga attagccgat gcgagagctg
     2341 aaaatgaaac tctgcgtgct gatgttgccg ctggtcgtaa gcgcctgcgg atcaacgcca
     2401 cctgctccgg taccgtgcgt gaagccaccg gcacctccgg cgtggataat gcaaccggcc
     2461 cccgactggc agacaccgct gaacgggatt atttcatcct cagagaacgg ctgatggcaa
     2521 tgcagaagca actggaagga gcacaggaat atatccgtac ccagtgtata ccgtgatgtt
     2581 ttgttatgag ggtgttactg gtaacgttaa ggtaatttaa caaagagtca gttccggact
     2641 ttatagtgtg ctcagttcat ggccaaaaac gatttctgtg ataaatattt tgaatattat
     2701 ttacaggtaa atggagtggg gcacatggat agaaatatta caatagagta tgaagtatat
     2761 gcccgtattg tatgggcaga gaaggcaaaa acatggtaat tccgtgtgtt gccatgatac
     2821 ctgattggca gaattgttgt ttggttttga gtatatagtc agcgtctttt gttcggtaat
     2881 tgctctttca attaaaatgc cagatatgat ttgcttttct ttgttgttta gtttttttgt
     2941 atattatttt tattgttttt atataattag ttttttattg ttgtcttatt aaggacggta
     3001 aattcaggat ggcagtctgt agataaacgg aggttactta tgctacatga tcacctggca
     3061 gaatgtctgg agaaaaaagg actgtaccgg agagcagctg aacgatgggc aaaagtgatg
     3121 gtacagctaa gtgatgacca gaaaagaaaa gtggcggcac agaaacgagc agagtgtttg
     3181 cgtaaggcgc gccggactcc ggtttcaccg gtgaacctga c
//
LOCUS       DAZKIP010000252         3220 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZKIP010000252 DAZKIP010000000
VERSION     DAZKIP010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.254"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..454
                     /gene="ehxC"
                     /locus_tag="ACHOUK_004824"
                     /gene_synonym="hlyC"
     CDS             <1..454
                     /gene="ehxC"
                     /locus_tag="ACHOUK_004824"
                     /gene_synonym="hlyC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enterohemolysin-activating
                     lysine-acyltransferase EhxC"
                     /protein_id="HFP9900079.1"
                     /translation="HKKWPLSVFAINVIPAIQTNQFALLIKDELPVAFCSWASLDLEC
                     EVKYINDVTSLYAKDWMSGERKWFIDWIAPFGHNMELYKYMRKKYPYELFRAIRLDES
                     SKTGKIAEFHGGGIDKKLASKIFRQYHHELMSEVKNRQDFNFNIEKEN"
     gene            456..>3220
                     /gene="ehxA"
                     /locus_tag="ACHOUK_004825"
     CDS             456..>3220
                     /gene="ehxA"
                     /locus_tag="ACHOUK_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin EhxA"
                     /protein_id="HFP9900080.1"
                     /translation="MTVNKIKNIFNNATLTTKSAFNTASSSVRSAGKKLILLIPDNYE
                     AQGVGINELVKAADELGIEIHRTERDDTAIANQFFGAAEKVVGLTERGVAIFAPQLDK
                     LLQKYQKVGSKIGGTAENVGNNLGKAGTVLSALQNFTGIALSGMALDELLRKQREGED
                     ISQNDIAKSSIELINQLVDTVSSINSTVDSFSEQLNQLGSFLSSKPRLSSVGGKLQNL
                     PDLGPLGDGLDVVSGILSAVSASFILGNSDAHTGTKAAAGIELTTQVLGNVGKAVSQY
                     ILAQRMAQGLSTTAASAGLITSAVMLAISPLSFLAAADKFERAKQLESYSERFKKLNY
                     EGDALLAAFHKETGAIDAALTTINTVLSSVSAGVSAASSASLIGAPISMLVSALTGTI
                     SGILEASKQAMFEHVAEKFAARINEWEKEHGKNYFENGYDARHAAFLEDSLSLLADFS
                     RQHAVERAVAITQQHWDEKIGELAGITRNADRSQSGKAYINYLENGGLLEAQPKEFTQ
                     QVFDPQKGTIDLSTGNVSSVLTFITPTFTPGEEVRERKQSGKYEYMTSLIVNGKDTWS
                     VKGIKNHKGVYDYSKLIQFVEKNNKHYQARIISELGDKDDVVYSGAGSSEVFAGEGYD
                     TVSYNKTDVGKLTIDATGASKPGEYIVSKNMYGDVKVLQEVVKEQEVSVGKRTEKIQY
                     RDFEFRTGGIPYDVIDNLHSVEELIGGKHDDEFKGGKFNDIFHGADGNDYIEGNYGND
                     RLYGDDGDDYISGGQGDDQLFGGSGNDKLSGGDGNNYLTGGSGNDELQAHGAYNILSG
                     GTGDDKLYGGGGIDLLDGGEGNDYLNGGFGNDIYVYGQNYGHHTIADEGGKGDRLHLS
                     DISFDDIAFKRVGNDLIMNKAINGVLSFNESNDVNGITFKNWFAKDASGADNHLVEVI
                     TDKDGRE"
BASE COUNT         1064 a          484 c          773 g          899 t
ORIGIN      
        1 gcataaaaaa tggccattat ctgtttttgc tattaatgtc atacctgcta ttcagacaaa
       61 tcaatttgct ctcttaataa aggatgagct tcctgtagct ttttgtagct gggccagttt
      121 agatctggaa tgtgaggtaa aatatataaa tgatgtaaca tctttgtatg caaaagactg
      181 gatgtcggga gaaagaaagt ggtttattga ctggatagct ccatttggac acaacatgga
      241 gttatataaa tacatgagga aaaagtatcc ttatgagtta ttcagagcaa taaggttaga
      301 tgagagcagt aaaacaggaa aaatagctga atttcatgga ggggggatag ataaaaaact
      361 ggcatcaaaa atattcaggc aataccatca tgaacttatg tctgaagtaa aaaacagaca
      421 agattttaat tttaatattg agaaagaaaa ctaatatgac agtaaataaa ataaagaaca
      481 ttttcaataa tgcgacattg actacaaaat cagcatttaa tacagcatca tcaagcgtac
      541 gttccgctgg aaaaaaactc atattattaa tacctgataa ttatgaagct cagggcgtgg
      601 gtattaatga gttggtcaaa gctgctgatg agcttggaat agaaatacac cgtactgaac
      661 gagatgatac agcgattgca aaccagtttt ttggtgcagc agaaaaagtt gtaggattaa
      721 ctgaacgtgg tgttgcaata ttcgcaccac aacttgacaa acttctgcag aagtatcaga
      781 aagttgggag taaaatagga ggaaccgctg aaaatgtagg taataatctg ggaaaagccg
      841 gaacagttct ctcagcacta cagaatttta cggggattgc tttatcaggc atggctcttg
      901 atgaattgct gagaaaacaa cgggaaggag aggatataag tcagaatgat attgccaaaa
      961 gtagtattga acttattaat cagcttgtag atacagtatc aagtataaac agtaccgttg
     1021 attcattttc tgagcagctt aaccagcttg gctcattttt atccagtaaa cctcgattaa
     1081 gttctgttgg tgggaaatta caaaatttac cagacctggg ccccctgggg gatgggctgg
     1141 atgttgtctc cggaattctt tctgctgtat cagcaagctt tattctggga aacagtgacg
     1201 cacatacagg aacaaaagct gcagcgggta tcgaactgac aactcaggtt cttggaaatg
     1261 ttggtaaagc tgtttcgcaa tatattctgg ctcagagaat ggcacagggg ttatcgacaa
     1321 cagctgcaag tgcgggtctg atcacatcgg ctgttatgct ggctatcagt cctctttctt
     1381 tcctggctgc tgcagataaa tttgagcgag ctaagcagct tgaatcatat tctgaacgat
     1441 ttaaaaaatt gaattatgaa ggggatgctt tactcgcagc ctttcataaa gaaaccggag
     1501 ctatagatgc agccctgaca acaataaata ctgtcctgag ttctgtatct gcgggagtta
     1561 gtgcagcctc cagtgcatcc ctcatagggg ccccgataag catgctggtg agtgcattaa
     1621 ccggtacgat atctggcatt ctggaagcat caaaacaggc tatgtttgag cacgttgcag
     1681 agaaattcgc tgctcggatc aatgaatggg aaaaggagca tggcaaaaat tattttgaga
     1741 atggatatga cgcaagacat gctgcgtttt tagaagactc tctgtctttg cttgctgatt
     1801 tttctcgtca gcatgcagta gaaagagcag tcgcaataac ccagcaacat tgggatgaga
     1861 agatcggtga acttgcaggc ataacccgta atgctgatcg cagtcagagt ggtaaggcat
     1921 atattaatta tctggaaaat ggagggcttt tagaggctca accgaaggag tttacacaac
     1981 aagtgtttga tcctcaaaaa gggaccatag acctttcaac aggtaatgta tcaagtgttt
     2041 tgacatttat aacaccaaca tttaccccag gagaagaagt tagagaaaga aaacagagtg
     2101 gtaaatatga atatatgaca tctcttattg taaatggtaa ggatacatgg tctgtaaaag
     2161 gcataaaaaa tcataaaggt gtatatgatt attcaaaatt gattcagttt gttgaaaaga
     2221 ataacaaaca ctatcaggcg agaataattt ctgagctcgg agataaagac gatgtggttt
     2281 attctggagc aggctcatca gaagtatttg ctggtgaagg ttatgatacc gtatcttata
     2341 ataagacgga tgttggtaaa ctaacaattg atgcaacagg agcatcaaaa cctggtgagt
     2401 atatagtttc aaaaaatatg tatggtgacg tgaaggtatt gcaggaagtc gttaaggaac
     2461 aggaggtgtc agtagggaag cgaacagaga aaatacaata tcgtgatttt gaattcagaa
     2521 ccggtggaat tccttatgat gtaatagata atcttcattc tgttgaagag ctcattggcg
     2581 gaaaacatga tgatgaattc aaaggcggta agtttaatga tatattccat ggcgcagatg
     2641 ggaacgatta tatcgaaggt aattatggta atgatcgact atacggcgat gatggggatg
     2701 attatatatc cggaggacag ggagacgacc agttatttgg tggtagtgga aacgataaat
     2761 tgagtggagg ggatggtaat aattatctga caggaggaag cggtaatgat gagcttcagg
     2821 cacacggagc ttataatatt ctgtcaggtg gtactggtga tgataaactt tatggtggtg
     2881 gtggtattga tcttctggat ggaggggaag gtaatgacta tctgaatggt ggttttggta
     2941 atgatattta tgtttatggg caaaactatg gtcatcatac aattgcagat gaaggaggta
     3001 aaggagatcg tttgcactta tctgatatta gctttgatga tatcgcattt aagagagttg
     3061 gaaatgatct tatcatgaat aaagccatta atggtgtact ttcatttaat gagtcaaatg
     3121 atgtcaatgg gataacattt aaaaactggt ttgcgaaaga tgcctcagga gcagataatc
     3181 atcttgttga ggttataaca gataaagatg gtcgagagat
//
LOCUS       DAZKIP010000253         3162 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZKIP010000253 DAZKIP010000000
VERSION     DAZKIP010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.255"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(5..631)
                     /gene="dsbA"
                     /locus_tag="ACHOUK_004826"
     CDS             complement(5..631)
                     /gene="dsbA"
                     /locus_tag="ACHOUK_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFP9900081.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(648..1634)
                     /gene="srkA"
                     /locus_tag="ACHOUK_004827"
     CDS             complement(648..1634)
                     /gene="srkA"
                     /locus_tag="ACHOUK_004827"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFP9900082.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(1711..1980)
                     /locus_tag="ACHOUK_004828"
     CDS             complement(1711..1980)
                     /locus_tag="ACHOUK_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFP9900083.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            2050..2634
                     /gene="mobA"
                     /locus_tag="ACHOUK_004829"
     CDS             2050..2634
                     /gene="mobA"
                     /locus_tag="ACHOUK_004829"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFP9900084.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIAMVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            2616..3143
                     /gene="mobB"
                     /locus_tag="ACHOUK_004830"
     CDS             2616..3143
                     /gene="mobB"
                     /locus_tag="ACHOUK_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFP9900085.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQNQDG"
BASE COUNT          827 a          768 c          763 g          804 t
ORIGIN      
        1 tgaattattt tttctcggac agatatttca ctgtatcagc atactgctga acaaaaacat
       61 ccatattgct ggtatccata ccctgcggat tcagctgata tttaccgtta acaaacatcg
      121 ccggaacacc acgcaattgc acgtcagctg cagctttttc ctgctgagcg accagagatt
      181 tcaccacgaa gctgttccac gccgcgtcgt actcttcacc tttaatacct gcgttgataa
      241 atacatcgcg gatatcagat gctgaacgaa tggtctgggt tttctgtacg ccttcaaaca
      301 gcggaacagt cactttgtct tccacgccca gcgccatcgc cacagcccat gcctgagtca
      361 gatctttgcc caggtcacca cccatgaagt tgacgtggta tttagtcatc ttcacgcctt
      421 ccggcagttt tttcttcaca ttatcagaaa tatgcagaac ttcttcaaac tgatagcagt
      481 gcgggcagaa gaaagagaaa aactccagca cttgcggcgc gccagctacc ggtttttcca
      541 gggtagtgta ctgtttacca tcttcatact gcgccgccga tgcgctaaac gctaaaacta
      601 aaccagccag cgccagccaa atctttttca tgatctactc tctccgatta atacataggt
      661 gttaattgca aagggggttc ttgtagaact tttgcctgtt ctataaaagt cgccgtctgt
      721 cgtagccagt aatcttcccc ggttaaccac gggaaatttt tcgggaacgc gggatcagcc
      781 caacgccgca ttagccaggc aagataataa accaaacgca tggcgcgtaa aggttcaatc
      841 agtccgattt cagcggtgtc gaactcgcta aattcttcat aagcttcaat aatagtttcc
      901 agttgcatcc gctgctcggc tttatcgcca ttgagcaaca tccacaaatc ctgaatagct
      961 ggaccattac gtgcatcatc cagatcaaca aacattggac catcgcgcca gagaatattc
     1021 ccggcgtggc agtctccatg tagccgcaga acggtgaaat cttcccgcca gtgtgctgta
     1081 acggcggcga tcagctcatc tgtcgctttc aggaatgccg ctttcaaccc ggaaggtatc
     1141 agtgtagcgt cctcaaacag cttgcgtggc tcaatgagat attcattcag accgatggtc
     1201 gggcgatgga taaaaagctg tttgcgcccc gtctggtgca tacgccctaa ataacgccca
     1261 accgcttcca tctgatcgat attatcagct tcgaactggc gaccaccgac gcttggaaaa
     1321 acagcgaaat aaaatccctg atgattcaat aaagtctgac cgttaaaagc cacaggtgct
     1381 gcaaccggaa cttcatcatt taccagctgc aacgcaaatt gatgttcttc gaggatttga
     1441 tcggctgtcc aacgttcagg acgataaaat ttgacgacaa aacgtcgacg atcttcgtcc
     1501 tgaaattgat agacacggtt ttcatagctg ttaagcgggg taagaccgga atccacccgg
     1561 atcccttgct caaacagagc gtccatgatg gtatccgggt gtagtgtctg gaaagtaaaa
     1621 gcgctgttgt tcatcccatc atccggaaaa cattacgaat gatgaaggat atcatttcac
     1681 gatgattttg gtggcgccgc ttacaagctt ttactcttta attacgccgc gagcgcgtaa
     1741 cagcgccgtt ttgaagtctt cttcataatc tttctgcaaa cccgggatca ccgcatcttt
     1801 cgcggaatca cgcattttca ggtgatagat cagaatgtca tccgtcaaat ccgccagttc
     1861 gccgtcaaaa cctgactctt tcgccagttt ctgcaaaaat tgcaacaggt taaggtctgg
     1921 ctctttttgc caggctggct ggaggagttc aataacttca ttcagacgtt tacatttcat
     1981 agtaatgctc cttactcttg agacagacac gttagcaggg tcaatcccac aataaaagag
     2041 gcgatatcgg tgaatctgat gacgacgata acaggcgttg tgctggcagg cggtaaagcc
     2101 agacgaatgg gcggcgtaga taaaggattg ctggacttaa atggcaaacc gctgtggcaa
     2161 catgtcgctg acgcgcttat gacgcagctc tctcacgtcg tggttaatgc taatcgtcat
     2221 caggaaatct atcaggcagg cggtctgaaa gtgattgaag attcactggc ggattaccca
     2281 ggccctctgg cagggatgct ttcagtaatg cagcaggaag ctggtgagtg gtttttgttt
     2341 tgtccgtgcg atacgcctta cattccccat gatttagccg cccggcttaa tcatcagcgc
     2401 aaagatgcgc ctgtcgtgtg ggtccatgac ggtgaacgcg atcacccgac tattgctatg
     2461 gtaaaccgcg ctattgagcc tttattacta gaatatctgc aagcaggaga acgccgggta
     2521 atggcattta tgcgtctggc tggcggtcat gcggttgatt tcagcgatca taaagatgca
     2581 tttgttaacg tgaatacgcc agaggagctt gcccgatggc aggaaaaacg atgataccgt
     2641 tactcgcctt tgccgcgtgg agtggtaccg gaaaaactac gcttttgaaa aaactgatcc
     2701 cggcattatg cgccagaggg atccgtccag ggctgattaa gcatacgcac catgatatgg
     2761 atgttgataa gccaggcaaa gatagctatg agctgcgcaa ggctggcgcg gcacaaacca
     2821 tcgttgccag ccagcaacga tgggccttga tgacggaaac accagacgaa gaagagctgg
     2881 atctacagtt tctcgcaagt cgaatggata cctcaaagct ggatttgatt ctggtcgaag
     2941 ggtttaagca tgaagagatc gcaaagattg tgctgtttcg cgatggagcc ggacatcgac
     3001 ctgaagaatt agtgatagac aggcatgtta ttgctgtagc cagtgatgtg ccgcttaatc
     3061 ttgatgtcgc attactggat attaatgatg ttgaggggct ggctgatttt gtggtggagt
     3121 ggatgcaaaa tcaggacgga taagcaagag taggctggag ca
//
LOCUS       DAZKIP010000254         3061 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZKIP010000254 DAZKIP010000000
VERSION     DAZKIP010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.256"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..1286
                     /gene="sctN"
                     /locus_tag="ACHOUK_004831"
     CDS             <1..1286
                     /gene="sctN"
                     /locus_tag="ACHOUK_004831"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system ATPase SctN"
                     /protein_id="HFP9900086.1"
                     /translation="LKLLNKYSYLHSINGSLIEAELDDVSVGEVCEIYASRQANERIA
                     RAQVVGFRNGKTLLNLIGSSVGLTRTAVLKPTGEQLTIQISDAFLGSVLNASGQIMER
                     FVPDTPGDRGNLRLIDELPPSYQERRVINTPLETRIRVIDGVLTCGIGQRVGIFASAG
                     CGKTVLMHMLVNNTEADVFVIGLIGERGREVTECAESLKKSVNAAKCVLVYATSDFSS
                     VDRCNAALMATTVAEYFRDRGKRVVLFIDSMTRYARALRDMKLAAGEPPARRGYPASV
                     FDSLPRLLERPGPTLKGSITEFYTVLLEGEDESDPLGDEIRSILDGHIYLSRKLAGQG
                     HYPAIDVLKSVSRVFGQVTDEKHRDNAARVRKNLTTLEDLQVFIDLGEYRAGQNAEND
                     FAMNARPKLTNWLKQSVNEKMPMSETLKELERIVK"
     gene            1276..1734
                     /locus_tag="ACHOUK_004832"
     CDS             1276..1734
                     /locus_tag="ACHOUK_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HFP9900087.1"
                     /translation="MLSKVNRLIRRTAQSLAACEASLQKLNAEKEKLAEKERLYDMQL
                     KNLQSLLDMKELLGEVVFRQDIFYSLRKVTVIQQQIAEINLEKQKIAERRKILNKEIV
                     QQQAQRKHWWLKGEKYDRLKKRIKKQLLNQMLYQDELEQEEKYNGRSQEN"
     gene            1712..1945
                     /locus_tag="ACHOUK_004833"
     CDS             1712..1945
                     /locus_tag="ACHOUK_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900088.1"
                     /translation="MDEVKKIEHCKILNTDTPDNSASDLDSFLKKNKKNKEMSEIVAG
                     TILQLLRQTQDNQFSTLGKKNGHMKSIDNKSSL"
     gene            2018..2587
                     /locus_tag="ACHOUK_004834"
     CDS             2018..2587
                     /locus_tag="ACHOUK_004834"
                     /inference="COORDINATES: protein motif:HMM:NF014559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     determinant, SpaN/EivJ family"
                     /protein_id="HFP9900089.1"
                     /translation="MTSRDQGINSELSNRTIQFKEKIHNGIHTEYITDQKHSNNKDRE
                     KKYRDGDKINGPQAHSLDITNERRFADNRTMFTQHIEKQRNVNTLNQNDINNSANNAN
                     VRENELTYQFQRWGQNHTVRILESSEGIRLKPSDTLVSDRLHEAQHNDVTAQRWVLTE
                     QDERQGQRHQPHEEQENEGKFENDQKDES"
     gene            2547..>3061
                     /locus_tag="ACHOUK_004835"
     CDS             2547..>3061
                     /locus_tag="ACHOUK_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
                     /protein_id="HFP9900090.1"
                     /translation="MKANSKTIRRMKVNVRSEESKSKHSTFETTFQNWKENGEDVALL
                     MPEFSAKWLPIAEESGSWSGWVLLREIFPLISAELAGMALMPETERLIGEWLSLSSSP
                     LNLKYPELKYNRLCVGKVFDGVLSPAQPLIRIWTGELNLWLDKVTVCQYENAPTLDKK
                     SLYWPIHFVIG"
BASE COUNT         1045 a          558 c          692 g          766 t
ORIGIN      
        1 aattaaaatt attaaataaa tacagctact tacatagcat aaacggctct ctgattgaag
       61 cagaacttga cgacgtttct gtgggtgaag tatgcgaaat ttatgcgagc aggcaagcaa
      121 atgaacgtat agccagggct caggtcgttg gctttcgcaa tgggaagaca ttattaaacc
      181 ttataggcag ctctgtgggg cttacacgta ctgcagtttt gaaaccaacg ggagagcagt
      241 taaccataca gatcagtgat gcctttcttg gttcggtttt gaatgcctct ggtcaaatta
      301 tggaaaggtt tgtcccggac actccaggcg ataggggaaa tttacgcctg attgatgaac
      361 tgccaccgtc gtatcaggaa cgtcgagtta ttaatacacc acttgagaca cggataagag
      421 tcattgatgg cgtactgaca tgtggcattg gtcaacgtgt tgggattttc gcctcggctg
      481 gctgtggcaa aacggtttta atgcatatgc tcgtaaacaa tactgaagct gatgtgtttg
      541 ttattggctt gattggcgaa cgtggtaggg aagttacgga atgcgcggaa tcgctcaaaa
      601 aatcagtaaa tgcagcgaaa tgcgtattgg tctatgccac ctctgatttt tcgtcagtag
      661 accggtgtaa tgccgccctg atggcgacaa ccgtggcaga gtattttcga gatcgaggaa
      721 agagagttgt actttttatc gactcaatga cgcggtatgc acgagcacta cgtgatatga
      781 aactggcagc gggtgaacca cctgccagac gtggttatcc tgcttctgtt tttgacagtt
      841 tgcctcgctt actggagagg ccgggaccaa cactaaaagg aagtattacc gagttttata
      901 ccgtgttact ggaaggggaa gatgaatcag acccactggg tgacgaaatt cgctcaatcc
      961 ttgatggcca tatctatcta agtcgcaaac tcgcgggtca aggtcactat ccggcaatag
     1021 atgttctaaa aagtgtgagt cgagtatttg gtcaggtcac tgatgaaaaa cacagagata
     1081 atgctgcacg ggtaaggaag aacctcacaa ctttggaaga tctacaggtg tttattgact
     1141 taggggaata tcgcgcaggg caaaatgctg aaaatgattt tgctatgaat gcccggccta
     1201 aactcacaaa ttggttgaaa cagtcagtta atgaaaaaat gccaatgagt gaaacgctaa
     1261 aagagcttga acgaattgtt aagtaaagtt aatcgactta tcagaagaac agcgcagagc
     1321 ctggctgcat gtgaagcatc attacaaaag ttaaatgcag aaaaagaaaa gttagccgaa
     1381 aaggaacgcc tgtatgatat gcaacttaaa aacctgcagt cactgttaga tatgaaggag
     1441 ttactcggcg aagttgtatt tcgccaggat atattttact cgttacgtaa agttacagtc
     1501 attcagcagc agatcgctga gataaatctt gaaaagcaaa aaatagccga gcggcgaaaa
     1561 atacttaaca aggaaatagt tcaacagcaa gcacaaagaa aacactggtg gctaaaagga
     1621 gaaaaatacg accgactaaa aaagcgcatc aaaaaacaac ttttaaatca gatgctttat
     1681 caagacgagc ttgaacagga ggaaaaatat aatggacgaa gtcaagaaaa ttgaacattg
     1741 taaaatttta aacacagaca cacccgataa ttctgcttcg gatttagaca gtttcttaaa
     1801 gaaaaacaaa aaaaataaag aaatgagtga gattgtcgct ggaaccattc tccagttatt
     1861 acgtcagact caggataacc aattttctac gttggggaaa aagaatgggc acatgaagtc
     1921 tattgacaac aaatcgtctc tctaggttga caggaatcca tctctttctt tgcaaacaaa
     1981 aggagccgag caattttcta caaagaaact tctcaatatg acttcacgag atcagggtat
     2041 taattctgaa ctatctaatc gaacgataca atttaaggaa aaaatccata atggtatcca
     2101 tacagaatac atcacagatc aaaaacacag taataataaa gatcgtgaaa aaaaataccg
     2161 cgatggagac aaaataaatg gaccgcaggc ccattcatta gacattacta acgaaagaag
     2221 gtttgctgat aacagaacca tgtttactca acatatcgaa aagcaaagga atgttaatac
     2281 gctcaatcag aatgatataa ataattctgc taataatgcc aatgtgcgcg aaaatgaatt
     2341 gacatatcag tttcagcgtt ggggacaaaa tcatacggtc cggattctgg aaagtagtga
     2401 agggatacga ctaaaaccat cagacacact tgtctccgac agactccatg aagcccaaca
     2461 caatgatgtt acagcacaac gttgggtatt gactgagcaa gatgaacgac aaggacaacg
     2521 acatcagcct catgaggagc aagaaaatga aggcaaattc gaaaacgatc agaaggatga
     2581 aagttaatgt tcggtctgag gaaagtaaat cgaaacactc tacgtttgaa accacttttc
     2641 aaaattggaa agaaaatggc gaagatgttg cattattaat gcccgaattt agtgctaaat
     2701 ggctaccgat agcagaagag agtggcagct ggtctggatg ggtgttgctt cgagaaatat
     2761 ttcccttaat ttctgccgag ctcgctggga tggctttaat gccagaaacg gaaaggttaa
     2821 taggggaatg gctcagttta tccagttccc cgttaaatct aaagtatcct gaactaaaat
     2881 ataatcgttt gtgcgtaggt aaagtatttg atggagtatt gagtccagct caaccactga
     2941 taagaatatg gacaggggaa ttgaatcttt ggttagataa agtcacagtc tgccaatacg
     3001 aaaacgctcc aactttagac aaaaaatcat tatattggcc cattcatttt gtaattggat
     3061 t
//
LOCUS       DAZKIP010000255         2989 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZKIP010000255 DAZKIP010000000
VERSION     DAZKIP010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.257"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..219
                     /locus_tag="ACHOUK_004836"
     CDS             <1..219
                     /locus_tag="ACHOUK_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP9900091.1"
                     /translation="ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDR
                     CSKCMRGCEMRGMVANFGGFLSINKLSQ"
     gene            230..973
                     /locus_tag="ACHOUK_004837"
     CDS             230..973
                     /locus_tag="ACHOUK_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP9900092.1"
                     /translation="MTQTESAILVHARRCAPAESCGFVIGTPEGERYQPCVNISAEPE
                     AYFRIAPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALSWWLVCRGEIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIDMPDFEREDDWWRNGQNLYLDNMAVT
                     GFYRVPLSSAQAGDILLCCFGASVANHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSAWRHRHWHVSAFTGIYNDLAAASACM"
     gene            970..1551
                     /locus_tag="ACHOUK_004838"
     CDS             970..1551
                     /locus_tag="ACHOUK_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP9900093.1"
                     /translation="MNTAAEAIRALSLQVPGFRRQMNEGWYQIRIRGEDTAPEAVYAR
                     LHEPLGEGAVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMALWGSALAAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKAKTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGDGKVVVIGRQA"
     gene            complement(1742..2269)
                     /gene="sodC"
                     /locus_tag="ACHOUK_004839"
     CDS             complement(1742..2269)
                     /gene="sodC"
                     /locus_tag="ACHOUK_004839"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFP9900094.1"
                     /translation="MKCKIIAAIAMLTAASCGYAAEQEVPMNLVSADGKEVSIGKITI
                     QETPYGLLFTPALHSLSEGIHGFHVHEKGNCAPALKDGKPVAALSAGGHFDPKNTGKH
                     LGPWSPDGHLGDLPALFVTHDGKANYPVLAPRLNSLKEIKGRSLMLHAGGDNHHDHPE
                     PLGGGGARMACGIIQ"
     gene            2403..>2989
                     /locus_tag="ACHOUK_004840"
     CDS             2403..>2989
                     /locus_tag="ACHOUK_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFP9900095.1"
                     /translation="MGKGGGRAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLLETTSKGDRNPSSVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPPRPFNIRMVRETADSTSDQLQN"
BASE COUNT          658 a          759 c          915 g          657 t
ORIGIN      
        1 gcgctgtttc ccggtcgcat catgctggcg aacacctgta tgtgggatta ccggggcgat
       61 gaatgcgggt ataacggtcc ggcagtggcg gatgagttcg acaaccccac cacggatatc
      121 cggaaggaca gatgcagtaa atgcatgcgc gggtgtgaga tgcgcggcat ggtggctaat
      181 tttggcggtt tcctttccat caataaactt tcgcagtaaa tcctgtttta tgacacagac
      241 tgaatcagcg attctggtgc atgcccggcg gtgtgcgcct gcggagtcgt gcggcttcgt
      301 gataggcacc ccggagggcg aacggtacca gccctgcgtg aatatctccg cagagccgga
      361 ggcgtatttt cgtattgcgc cggaagactg gctgcaggca gagatgcagg gggagattgt
      421 ggcactggtc cacagccacc ccggtggtct gccctggctg agcgaggcgg accggcggct
      481 gcagataaaa agtgcactgt cctggtggct ggtctgccgg ggggaaattc ataaattccg
      541 ctgtgtgcca catctgacag gacggcgctt tgagcacggg gtgacggact gttacacgct
      601 gttccgggat gcctaccatc tggcgggaat tgatatgccg gattttgagc gtgaggatga
      661 ctggtggcgt aacggtcaga acctttacct ggacaatatg gcggtcaccg gcttttaccg
      721 ggtgcccctg tcctctgcac aggcgggcga tatcctgctg tgctgctttg gcgcatcggt
      781 ggccaatcat gccgccattt actgcggcaa cggtgaactg cttcaccatc tgcctgaaca
      841 actgagtaaa cgggagaggt attccgaaaa atggcaacga cgaacgcatt cagcctggcg
      901 tcaccgccac tggcacgtat ctgccttcac ggggatttac aacgatttag ccgccgcctc
      961 agcctgtatg tgaacacggc agcggaagcc atccgggcgc tgtcgttaca ggtgccggga
     1021 ttccgccgtc agatgaacga aggctggtac cagatacgta ttcgcggtga ggacacggca
     1081 ccggaggcgg tgtacgcccg tcttcacgaa cctctgggtg agggggcggt catccatatt
     1141 gtgccgcgac tggccggagc cggaaagggc ggactgcaga ttgtgctggg ggcggcggcc
     1201 atcgtggggt cgttcttcac tgccggggca tcaatggcgt tatggggttc agccctggca
     1261 gccggtggtt tttctgccac cacgatgctg ttttcacttg gagccagcat gattctgggc
     1321 ggtgtggccc agatgctggc cccgaaggca aaaacaccgg attaccgcgc aacggataac
     1381 ggcagacaga acacgtactt ttcctcgctg gataacatga ttgcccaggg gaacccgatg
     1441 ccggtgcctt acggtgaaat gctggttggc tcccggcgaa tctcccagga catcagtacc
     1501 cgtgatgaag gcggtgacgg gaaggtggtg gttatcgggc ggcaggcata aaagcgaaaa
     1561 aatcccgcag tgctcacgga caggaactgc gggagcgtta cgaagattga gtgtaaggaa
     1621 ttattcttat gtcacgacaa aaacattaac tcagagaggg aggatgtgcc gttcttttca
     1681 gggagaaagg atttatcgtc ctgaggaata aaggtaaggg gcccgcccct tacctgactg
     1741 attattgaat gatgccgcag gccattctcg caccaccacc gcccaggggc tccggatggt
     1801 catgatggtt atcaccgcca gcatgaagca tgagagaacg ccctttaatc tcttttaatg
     1861 agttcagtct cggggccagg accgggtagt tcgcttttcc gtcatgcgtc acgaacagcg
     1921 cagggaggtc gcccaggtgt ccatccggag accaggggcc aagatgtttg ccggtgtttt
     1981 tcgggtcaaa gtgaccgcca gccgataatg ctgcgaccgg ttttccgtct ttcagtgccg
     2041 gggcgcaatt tcctttttcg tgcacatgaa aaccatgaat gccttcagac agagagtgaa
     2101 gggctggtgt gaacagcaga ccgtaggggg tctcctgaat ggttattttt ccaatgctga
     2161 cttcttttcc gtcagcactg acaaggttca ttgggacttc ctgttctgct gcgtatccgc
     2221 atgatgctgc tgtcagcatg gcaatggcag caatgatttt acatttcata aaaccctcat
     2281 taattccgtt aacagactga gcttgctggt tacagggtaa caaacagcgt tctgatgata
     2341 tcgcgcaata gctgtgcaat atcctatcac tgcgattaat aataccaatt gagaggaaca
     2401 ttatgggtaa aggtggcggc agggcgcaca cgccggttga ggcaaaggac aatcttaagt
     2461 ccacgcagat gatgagcgtg attgatgcca ttggtgaagg gccgattgaa ggtccggtga
     2521 aggggctgca gagtatcctg gtgaacaaaa ccccgctgac ggacacggac ggtaatcctg
     2581 tgatacatgg tgtgacagcg gtctggcgcg ccggggagca ggagcagaca ccacctgaag
     2641 gctttgagtc ttccggagct gaaaccgcac tgggcgtgga agtgacgaag gcaaagccgg
     2701 tgacgcgcac cattacatcc gcgaacattg accgcctgcg ggtcaccttc ggggtgcagt
     2761 cactgttgga gaccacctca aagggcgacc gtaatccctc ttctgtccga ctgctgattc
     2821 agttgcagcg taacggtaac tgggtgacgg aaaaggatgt caccattaac ggcaagacca
     2881 cctcgcagtt tctggcgtcg gtgattctgg ataatctgcc tccccgtcct tttaacatcc
     2941 ggatggtccg ggagacggcg gacagcacct cggaccagct gcagaataa
//
LOCUS       DAZKIP010000256         2876 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZKIP010000256 DAZKIP010000000
VERSION     DAZKIP010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.258"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            556..762
                     /locus_tag="ACHOUK_004841"
     CDS             556..762
                     /locus_tag="ACHOUK_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HFP9900096.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKHKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNGLNR"
     gene            838..1134
                     /locus_tag="ACHOUK_004842"
     CDS             838..1134
                     /locus_tag="ACHOUK_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFP9900097.1"
                     /translation="MNAYYIQDCLEAQSWARYYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            1140..1925
                     /gene="bet"
                     /locus_tag="ACHOUK_004843"
     CDS             1140..1925
                     /gene="bet"
                     /locus_tag="ACHOUK_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFP9900098.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            1922..2599
                     /locus_tag="ACHOUK_004844"
     CDS             1922..2599
                     /locus_tag="ACHOUK_004844"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFP9900099.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEIHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMASFDEMVPEFIEKMDEALAEIG
                     FVYGEQW"
     gene            2599..2781
                     /locus_tag="ACHOUK_004845"
     CDS             2599..2781
                     /locus_tag="ACHOUK_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFP9900100.1"
                     /translation="MKHPHDNIRVGTITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKRGAVCTKHLLLN"
     gene            2754..>2876
                     /locus_tag="ACHOUK_004846"
     CDS             2754..>2876
                     /locus_tag="ACHOUK_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFP9900101.1"
                     /translation="MHKASPVELRTSIGMAHSLAQIGVRFVPIPVETDEEFHTLA"
BASE COUNT          787 a          712 c          708 g          669 t
ORIGIN      
        1 aataagtcat ttatgtatca tttagcaact gactaaaaaa atacacataa gaactcagac
       61 taccaatatt cgattaaatt tgctgccaac ctaccgagtt caccacaacc cgatgcccct
      121 ctgtcacgag gcacattaaa gctgtacaaa tttcagccac accaataagt cttcatactc
      181 ttttttataa aaattcattt aattatcaat cagaacaagt atatcaaaca ttatcaatac
      241 taattgctat tgaccacacc aatagccatt gctattgtca tgctattgca tcgatgttgg
      301 tatcacaaaa tttataactt caccgttgcg atgaccgctt agatccgcag cttgaatttc
      361 agcaggctcc ggggagtgcg aggggtgaag cggacgcgtg aacgtcggtg tgaccagctg
      421 aaatcaactc aacacttcat acctcagtcg cttcaacgag gcgacttagt tatgacaacc
      481 ggcggccatc caccgcctga atacgcgcag aagtctctat atgttcagca gcccagctta
      541 cgggcaggag tttttatggt tcatcaacat tacggaacgc agaccgttaa tcgaggtgcg
      601 gtcatgccag gaatgctggt caaacacaaa gatggtacct ggactgcatc agctaattta
      661 cgcggacggc tttatctgca tcgcggcatc gagcgcactt atacccgtga tttgctcgtg
      721 gaagtttttc tcgacggacg cggtaacggc ctgaatcgct aatccccttt cctgttttcc
      781 taatcagcct ggcatttcgc gggcgatatt ttcacagcca ttttcaggag ttcagccatg
      841 aacgcttatt acattcagga ttgtcttgag gctcagagct gggcgcgtta ctaccagcag
      901 atcgcccgtg aagagaaaga ggcagaactg gcagacgaca tggaaaaagg cctgccccag
      961 cacctgtttg aatcgctatg catcgatcat ttgcaacgcc acggggccag caaaaaagcc
     1021 attacccgtg cgtttgatga cgatgttgag tttcaggagc gcatggcaga acacatccgg
     1081 tacatggttg aaaccattgc tcaccatcag gttgatattg attcagaggt ataaaacgga
     1141 tgagtacagc actcgcaacg ctggctggga agctggctga acgtgtcggc atggattctg
     1201 tcgacccaca ggaactgatc accactcttc gccagacggc atttaaaggc gatgccagcg
     1261 atgcgcagtt catcgcattg ttgatcgtcg ccaaccagta cggccttaat ccgtggacga
     1321 aagaaattta cgccttccct gataagcaga acggcatcgt tccggtggtg ggcgttgatg
     1381 gctggtcccg catcatcaac gaaaaccagc agtttgatgg catggacttt gagcaggaca
     1441 atgaatcctg cacatgccgg atttaccgca aggaccgtaa tcatccgatc tgcgttaccg
     1501 agtggatgga tgaatgccgc cgcgaaccat tcaaaacccg cgaaggcaga gaaatcacgg
     1561 ggccgtggca gtcgcatccc aaacggatgt tacgtcataa agccatgatt cagtgtgccc
     1621 gtctcgcctt cggatttgct ggtatctatg acaaggatga agccgagcgc attgtcgaaa
     1681 ataccgcata cactgcagaa cgtcagccgg aacgcgacat cactccggtt aacgatgaaa
     1741 ccatgcagga aattaacact ctgctgatcg ccctggataa aacatgggat gacgacttat
     1801 tgccgctctg ttcccagata tttcgccgcg acattcgtgc atcgtcagaa ctgacacagg
     1861 ccgaagcagt aaaagctctt ggattcctga aacaaaaagc cactgaacag aaggtggcag
     1921 catgacaccg gacattatcc tgcagcgtac cgggatcgac gtgagagctg tcgaacaggg
     1981 agatgatgcg tggcacaaat tacggctcgg cgtcatcaca gcttcagaaa ttcacaacgt
     2041 aatagcaaaa ccccgatcag gaaagaagtg gcctgacatg aaaatgtcct acttccacac
     2101 cctgctggct gaggtttgca ccggtgtggc tccggaagtt aacgctaagg ctctggcctg
     2161 gggaaaacag tacgagaacg acgccagaac cctctttgag ttcacttccg gcgtgaatgt
     2221 tactgaatcc ccgatcatct atcgcgacga aagtatgcgc accgcctgct ctcccgatgg
     2281 tttatgcagt gacggcaacg gccttgagct gaaatgcccg tttacctccc gggatttcat
     2341 gaagttccgg ctcggtggtt tcgaggccat aaaatcggct tacatggccc aggtgcagta
     2401 cagcatgtgg gtgacacgaa aagatgcctg gtactttgcc aactatgacc cgcgcatgaa
     2461 gcgtgaaggc ctgcattatg tcgtgattga gcggaatgaa aagtacatgg cgagttttga
     2521 cgagatggtg ccggagttca tcgaaaaaat ggacgaggca ctggctgaaa ttggttttgt
     2581 atatggggag caatggtaat gaagcatcct cacgataata tccgggtagg cacgatcact
     2641 ttcgtctact ccgttacaaa gcgaggctgg gtatttcccg gcctttctgt tatccgaaat
     2701 ccactgaaag cacagcggct ggctgaagag ataaataata aacgaggggc tgtatgcaca
     2761 aagcatctcc tgttgaatta agaacgagta tcgggatggc acatagcctc gctcaaattg
     2821 gagtcaggtt tgtgccaata ccagtagaaa cagacgaaga atttcatacg ttagcc
//
LOCUS       DAZKIP010000257         2854 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZKIP010000257 DAZKIP010000000
VERSION     DAZKIP010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.259"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..203)
                     /locus_tag="ACHOUK_004847"
     CDS             complement(<1..203)
                     /locus_tag="ACHOUK_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFP9900102.1"
                     /translation="MTVKISGVLKDGTGKPVQNCTIVLKARRTSSTVVVNTVASENPD
                     EAGRYSMDVEYGQYSVILLVEGFP"
     gene            complement(355..954)
                     /locus_tag="ACHOUK_004848"
     CDS             complement(355..954)
                     /locus_tag="ACHOUK_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP9900103.1"
                     /translation="MRKLCAVILSAVVWQVAAATPASAAEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFMDALGLITSFSYANAEDEQKTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFYGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVTIDLAYEGSGSGDWRSDAFIVGIGYRF"
     gene            complement(1022..>2854)
                     /locus_tag="ACHOUK_004849"
     CDS             complement(1022..>2854)
                     /locus_tag="ACHOUK_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFP9900104.1"
                     /translation="TLPDGVETYGVWGLSLPSLRRRLFRCVSVRENTDGTFAITAVQH
                     VPEKEAIVDNGARFEPQSGSLNSVIPPAVQHLTVEVSAADGQYLAQAKWDTPRVVKGV
                     RFSLRLTSGKGTDARLVTTAITADTEHRFSGLPLGEYTLTVRAINSYGQQGEPATTTF
                     RIAAPAAPSRIELTPGYFQITATPHLAVYDPTVQFEFWFSEKRIADIRQVETAARYLG
                     SALYWIAASINIKPGHDYYFYIRSVNTVGKSAFVEAVGRASDDAEGYLDFFKGEIGKT
                     HLAQELWTQIDNGQLAPDLAEIRTSITNVSNEITQTVNKKLENQSAAIQQIQKVQVDT
                     NNNLNSMWAVKLQQMQDGRLYIAGIGAGIENTPAGMQSQVLLAADRIAMINPANGNTK
                     PMFVGQGDQIFMNEVFLKYLTAPTITSGGNPPAFSLTPDGRLTAKNADISGNVNANSG
                     TLNNVTINENCRVLGKLSANQIEGDLVKTVGKAFPRDSRAPERWPSGTITVRVYDDQP
                     FDRQIVIPAVAFSGAKHEKEHTDIYSSCRLIVRKNGAEIYNRTALDNTLIYSGVIDMP
                     AGHGHMTLEFSVSAWLVNNWYPTASISDLLVVVMKKATAGITIS"
BASE COUNT          645 a          836 c          686 g          687 t
ORIGIN      
        1 ggaaatccct ccaccaacag aatgacgctg tactgaccgt actcaacatc catgctgtaa
       61 cgaccggctt catccggatt ttcagaggcc accgtgttca ccaccaccgt gctgctggtt
      121 cgtctggcct tcagcacaat ggtgcagttc tgtactggtt ttcctgtgcc atctttaagc
      181 acgccagaaa tttttactgt catacttttc caccaataaa aaaagcccgc agcagtgacg
      241 ccacgggctt caggacagtg taactttacg tttcctcaaa cgcagttcac cccataaggt
      301 ggatgaacct gcgtatcata acaatattta cagaagataa atcggcgtct gttgtcagaa
      361 acggtatccg ataccaacaa taaatgcatc cgatcgccag tcgccactac cggaaccttc
      421 ataagcaagg tcaatggtca cggattcggt cgggttaaac tgcacgccag ccccccacgc
      481 cagagacgtg ttgctgtggc gaccgtcatc acttccggtc agcacatcgt gcgttttccc
      541 cttgttgtca gttacgcgga gataatcccc gtagaaagtc gacacacggc tgtaagccac
      601 acccgccatc gcatacgcgc tgaaccattc attcacgcgt acagacggcc ccgccatcac
      661 gctgaaccag cggttacgca cggaatcttc atgccagcgg gtatcgctgt agcgcgtttt
      721 ttgctcatcc tcagcattgg cataactgaa ggacgtaatc agccccagcg cgtccataaa
      781 ctcataacgg tatttcacgt taatcccgtt cagatcatca ctaccgggaa cgttcgtcga
      841 ggcatggaga taccccgcgc tcagcgtgga ctgatgttct gctgcactcg ctggcgtagc
      901 agcggcgacc tgccagacta ctgcggacaa aataacagca cataatttac gcataattac
      961 ctctcgcttt tctgcaataa aaaaggcgcc atttctggcg cccgtatctg ggttataaaa
     1021 ttcagctaat cgtgatgcct gcagtggctt tcttcatcac cacaaccagc aaatcgctga
     1081 tacttgctgt gggataccag ttatttacca gccatgctga caccgaaaac tccagtgtca
     1141 tgtgaccgtg accggcaggc atatcaataa caccactgta aatcagcgta ttatccagcg
     1201 cggtacggtt ataaatttca gcaccgtttt tccgcactat cagacggcat gaggagtaaa
     1261 tatcagtatg ctctttctca tgtttagcgc cgctgaatgc caccgccgga ataacaatct
     1321 gccggtcaaa cggctgatcg tcataaaccc tgacggtaat ggttcctgat ggccaccgct
     1381 ccggtgcacg ggagtcccgg gggaaagctt tgcccactgt tttaacgaga tcgccttcaa
     1441 tctggttcgc ggacaatttt cccagaaccc gacagttctc gttaatcgtg acgttgttga
     1501 gcgtcccgga gttcgcattc acgttaccgc tgatatcggc atttttcgcc gtcagccgcc
     1561 cgtccggtgt cagggaaaat gccggaggat taccgccgct ggtaatggtg ggagccgtca
     1621 gatatttcag gaacacttca ttcataaata tctgatcgcc ctgaccaaca aacatcggct
     1681 ttgtgttgcc attcgcagga ttaatcatcg caatcctgtc tgccgccagc agcacctgac
     1741 tctgcattcc tgctggcgta ttctcaatac cggcaccgat acccgcaata taaaggcgtc
     1801 cgtcctgcat ctgctgcagc ttcactgccc acatgctgtt caggttatta tttgtatcaa
     1861 cctgaacttt ctgtatctgc tggattgccg cactctgatt ttccagtttt ttattgacgg
     1921 tctgcgtgat ttcattgctg acattcgtaa tggacgtcct gatttcagcc aggtccggcg
     1981 caagctgacc gttatcaatc tgcgtccaca gctcctgggc cagatgtgtt ttcccgattt
     2041 ctcctttgaa aaaatccagg taaccttccg catcatcgct cgcccgaccg acagcctcca
     2101 cgaatgccga tttgccaaca gtattcacac tgcggatata aaaataataa tcatggcccg
     2161 gtttgatatt gatactggca gctatccagt acagcgccga gccaagatag cgggctgcgg
     2221 tttcaacctg cctgatatcc gcaatccgct tttccgagaa ccagaactca aactgtaccg
     2281 tcgggtcata aacggcaaga tgcggcgtgg cggttatctg aaaatagccc ggcgtcagct
     2341 caatccgcga cggtgctgcc ggtgcggcaa tccggaaggt ggtggtggca ggttcaccct
     2401 gctggccata gctgtttatc gcccgcaccg tcagggtgta ttccccgagc ggcaggccgc
     2461 tgaaacggtg ctccgtgtct gcggtgatgg cggtggtcac cagtctggca tccgttccct
     2521 taccactggt caggcgcaga ctgaagcgca cgcccttcac cacccgcggc gtgtcccatt
     2581 tagcctgcgc cagatactgg ccgtcagctg cactcacctc caccgtcagg tgctgtactg
     2641 ccggtgggat gacgctgttc agggaacctg actgcggctc aaagcgggca ccgttatcca
     2701 cgatggcttc tttttccggt acgtgctgca ccgccgtgat ggcaaaggtg ccgtccgtgt
     2761 tttcccggac ggagacacag cggaacaggc gacggcgcag tgacggcagg gagagtcccc
     2821 acaccccgta tgtctccaca ccatcaggca gggt
//
LOCUS       DAZKIP010000258         2813 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZKIP010000258 DAZKIP010000000
VERSION     DAZKIP010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.260"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            31..1431
                     /locus_tag="ACHOUK_004850"
     CDS             31..1431
                     /locus_tag="ACHOUK_004850"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFP9900105.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKEGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
     gene            1802..2626
                     /gene="gadX"
                     /locus_tag="ACHOUK_004851"
     CDS             1802..2626
                     /gene="gadX"
                     /locus_tag="ACHOUK_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFP9900106.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKVSSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDDHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDCSAEGIRL"
BASE COUNT          744 a          636 c          665 g          768 t
ORIGIN      
        1 ttgccttcaa ataaatttaa ggagttcgaa atggaccaga agctgttaac ggatttccgc
       61 tcagaactac tcgattcacg ttttggcgca aaggccattt ctactatcgc ggagtcaaaa
      121 cgatttccgc tgcacgaaat gcgcgatgat gtcgcattcc agattatcaa tgatgaatta
      181 tatcttgatg gcaacgctcg tcagaacctg gccactttct gccagacctg ggacgacgaa
      241 aacgtccata aattgatgga tttgtcgatc aataaaaact ggatcgacaa agaagaatat
      301 ccgcaatccg cagccatcga cctgcgttgc gtaaacatgg ttgccgatct gtggcatgcg
      361 cctgcgccga aaaatggtca ggccgttggc accaacacca ttggttcttc cgaggcttgt
      421 atgcttggcg ggatggcgat gaaatggcgt tggcgcaagc gtatggaagc tgcaggcaaa
      481 ccaacggata aaccaaacct ggtgtgcggt ccggtgcaaa tctgctggca taaattcgct
      541 cgctactggg atgtggagct gcgcgagatc cctatgcgcc ccggtcagtt gtttatggac
      601 ccgaaacgca tgattgaagc ctgcgacgaa aacaccatcg gcgtggtgcc gactttcggc
      661 gtgacctaca ccggtaacta tgagttcccg caaccgctgc acgatgcact ggataaattc
      721 caggccgaca ccggtatcga catcgacatg cacatcgacg ctgccagcgg tggcttcctg
      781 gcaccgttcg tcgccccgga tatcgtctgg gacttccgcc tgccgcgtgt gaaatcgatc
      841 agtgcttcag gccataaatt cggtctggct ccgctgggct gcggctgggt gatctggcgt
      901 gatgaagaag cgctgccgca ggaactggtg ttcaacgttg actacctcgg tggtcagatt
      961 ggtactttcg ccatcaactt ctcccgcccg gcgggtcagg tgattgcgca gtattatgaa
     1021 ttcctgcgcc tcggtcgtga aggctatacc aaagtacaga acgcctctta ccaggttgcc
     1081 gcttatctgg cggatgaaat cgccaaactg gggccgtatg agttcatctg tacgggtcgt
     1141 ccggacgaag gcatcccggc ggtttgcttc aaactgaaag agggtgaaga tccgggatac
     1201 accctgtacg acctctctga acgtctgcgt ctgcgcggct ggcaggttcc ggccttcact
     1261 ctcggcggtg aagccaccga catcgtggtg atgcgcatta tgtgtcgtcg cggcttcgaa
     1321 atggactttg ctgaactgtt gctggaagac tacaaagcct ccctgaaata tctcagcgat
     1381 cacccgaaac tgcagggtat tgcccagcag aacagcttta aacacacctg ataacataac
     1441 gttgtaaaaa ccgaatgccc agcctttaaa aaaaacagct gggcattcgg ttgcttatta
     1501 atcgcaataa tatattggct gtctattcat cgtgttgata agatatatgt tatatgaatg
     1561 tttattaaat gtagcgatta cctttaatgt cacgacaagg gattatttgc ttactattaa
     1621 tttccctgcg ttcttaaaca cgtttttgta ttataaatcc agtcatcctg cccgtagaac
     1681 atcacacatt atcatcctgt tctcccgctt cgtttaaatt tatttatcaa tcaatttgac
     1741 ttaagagggc ggcgtgctac attaataaac agtaatatgt ttatgtaata ttaagtcaac
     1801 tatgcaatca ttacatggga attgtctaat tgcgtacgca agacataaat atattctcac
     1861 catggttaat ggtgaatatc gctattttaa tggcggtgac ctggtttttg cggatgcaag
     1921 ccaaattcga gtagataagt gtgttgaaaa ttttgtatta gtatcaaggg atacgctttc
     1981 attatttctc ccgatgctca aggaggaggc attaaatctt catgcacata aaaaagtttc
     2041 ttcattactc gttcatcact gtagcagaga tattcctgtt tttcaggaag ttgcgcaact
     2101 atcgcagaat aagaatcttc gctatgcaga aatgctacgt aaaagagcat taatctttgc
     2161 gttgttatct gtttttcttg aggatgatca ctttataccg ctgcttctga acgttttaca
     2221 accgaacatg cgaacacgag tttgtacggt tatcaataat aatatcgctc atgagtggac
     2281 actagcccga atcgccagcg agctgttgat gagtccaagt ctgttaaaga aaaaattacg
     2341 cgaagaagag acatcatatt cacagttgct tactgagtgt agaatgcaac gtgctttgca
     2401 acttattgtt atacatggtt tttcaattaa gcgagtcgca gtgtcctgtg gatatcacag
     2461 cgtgtcgtat ttcatttacg tctttcgaaa ttattatggg atgacgccca cagagtatca
     2521 ggagcgatcg gcgcagggat tgccgaaccg tgactcggcg gcaagtattg ttgcgcaagg
     2581 gaatttttac ggcactgact gttctgcgga aggaataaga ttatagagtt ttactcagac
     2641 ataaaaaaaa cccggcatag gggaccggga agaggatagt ctgccgtctc cagactaata
     2701 aaccgttata acactccctg ttggcacggg aaactttgtg ctctcagtaa gttaaatata
     2761 acttttactg gaaataagat cagccatttt tttataaaca taagctatac gct
//
LOCUS       DAZKIP010000259         2801 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZKIP010000259 DAZKIP010000000
VERSION     DAZKIP010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.261"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..570
                     /locus_tag="ACHOUK_004852"
     CDS             <1..570
                     /locus_tag="ACHOUK_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP9900107.1"
                     /translation="KTGAEAIRALATQLPVFRQKLSDGWYQVRITGRDVSTSGLTAQL
                     HETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGMT
                     GILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLYG
                     EMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            631..>2801
                     /locus_tag="ACHOUK_004853"
     CDS             631..>2801
                     /locus_tag="ACHOUK_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFP9900108.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQKVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGASFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAILRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDLSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTP"
BASE COUNT          604 a          738 c          921 g          538 t
ORIGIN      
        1 aaaacggggg ctgaagccat ccgcgcactg gccacacagc tcccggtgtt tcgtcagaag
       61 ctgagcgacg gctggtatca ggtacggatt accgggcggg acgtcagcac gtccgggtta
      121 acggcgcagt tacatgagac tctgcctgat ggcgctgtga ttcatattgt tcccagagtc
      181 gccggggcca agtcaggtgg cgtattccag attgtcctgg gggctgccgc cattgccgga
      241 tcattcttta ccgccggagc cacccttgca gcatgggggg cagccattgg ggccggtggt
      301 atgaccggca tcctgttttc tctcggtgcc agtatggtgc tcggtggtgt ggcgcagatg
      361 ctggcaccga aagccagaac tccccgtaca cagacaacgg ataacggcaa acagaacacc
      421 tatttctcct cactggataa catggttgcc cagggcaatg ttctgccggt tctgtacggt
      481 gaaatgcgcg tggggtcacg cgtggtttct caagagatca gcacggcaga cgaaggggac
      541 ggtggtcagg ttgtggtgat tggtcgctga tgcaaaatgt tttatgtgaa accgcctccg
      601 ggcggtttta tcgtttatgg agcatgacga atgggtaaag gcagcagtaa ggggcatacc
      661 ccgcgcgaag cgaaggacaa cctgaagtcc acgcagctgc tgagtgtgat cgatgccatc
      721 agcgaagggc cggttgaagg tccggtggat ggattaaaaa gcgtgctgct gaacagtacg
      781 ccggtgctgg acagtgaggg gaataccaat atatccggcg tcacggtggt gttccgggcc
      841 ggtgagcagg agcagacacc gccggaggga tttgaatcct ccggctccga gacggtgctc
      901 ggtacagaag tgaaatatga cacgccgatc acccggacca tcacgtcggc aaacattgac
      961 cgtctgcgtt ttactttcgg cgtgcaggca ctggtggaaa ccacctcaaa gggggacagg
     1021 aatccatcgg aagtccgcct gctggttcag atacaacgta acggtggctg ggtgacggaa
     1081 aaagacatca ccattaaggg taaaaccact tcacagtatc tggcctcggt ggtggtggat
     1141 aacctgccgc cgcgcccgtt taatatccgg atgcgcagga tgacgccgga cagcaccaca
     1201 gaccagctgc agaacaaaac gctctggtcg tcatacaccg aaattatcga tgtgaaacag
     1261 tgctacccga acacggcact ggtcggcgtg caggtggact cggagcagtt cggcagccag
     1321 aaggtgagcc gtaattatca tctgcgcggg cgtattctgc aggtgccgtc gaattataac
     1381 ccgcagacgc ggcaatacag cggtatctgg gacggaacgt ttaaaccggc atacagcaac
     1441 aacatggcct ggtgtctgtg ggatatgctg acccatccgc gctacggcat ggggaaacgt
     1501 cttggtgcgg cggatgtgga taaatgggcg ctgtatgtca tcggccagta ctgcgaccag
     1561 tcagtgccgg acggctttgg cggcacggag ccgcgcatca cctgtaatgc ctacctgacc
     1621 acacagcgta aggcgtggga tgttctcagc gatttctgct cggcgatgcg ctgtatgccg
     1681 gtatggaacg ggcagacgct gacgttcgtg caggaccgac cgtcggataa ggtgtggacc
     1741 tataaccgca gtaatgtggt gatgccggat gatggcgcgt cgttccgcta cagcttcagc
     1801 gccctcaagg accgccataa tgccgttgag gtgaactgga ttgacccgga taacggctgg
     1861 gagacggcga cagagcttgt ggaggacacg caggccattc tccgttacgg tcgtaacgtc
     1921 acgaagatgg atgcctttgg ctgtaccagc cgggggcagg cacaccgcgc cgggctgtgg
     1981 ctgattaaaa cggagctgct ggagacgcag accgtggatt tcagcgtggg cgcagaaggg
     2041 cttcgccatg taccgggcga tgtcattgaa atctgtgatg atgactatgc ggggatcagc
     2101 atcggtgggc gtgtgctggc ggtgaacagc cagacccgga cgctgacgct cgaccgtgaa
     2161 atcacgctgc catcctccgg taccacgctg ataagcctgg ttgacggaag tggcaatccg
     2221 gtcagcgtgg aggtccagtc cgtcaccgac ggcgtgaagg taaaagtgag ccgtgttcct
     2281 gacggcgttg ccggatacag cgtatggggg ctgaagttgc cgacgttgcg ccagcgcctg
     2341 ttccgctgcg tgagtatccg tgagaacgac gacggcacgt atgccatcac cgccgtgcag
     2401 catgtacccg aaaaagaagc catcgtggat aacggggcgc actttgacgg cgacctgagc
     2461 ggcacggtga atggcgtcac gccgcccgcg gtgcagcacc tgactgccga agtcaccgca
     2521 gacagcgggg aatatcaggt gctggcgcgc tgggacacgc cgaaggtggt gaagggggtg
     2581 agcttcctgc ttcgcctgac cgtggcagcg gacgatggca gtgagcggct ggtcagtacg
     2641 gccaggacga cggaaaccac ataccgcttc acgcaactgg cgctggggaa ctacaggctg
     2701 actgtccggg cggtaaatgc gtggggacag cagggcgatc cggcatcggt atcgttccgg
     2761 attgccgcac cggcagcgcc gtctcggatt gagctgacac c
//
LOCUS       DAZKIP010000260         2797 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZKIP010000260 DAZKIP010000000
VERSION     DAZKIP010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.262"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            82..2361
                     /gene="ydeP"
                     /locus_tag="ACHOUK_004854"
     CDS             82..2361
                     /gene="ydeP"
                     /locus_tag="ACHOUK_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFP9900109.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAQNTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSVEFLARLGERYGFTPPHVPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQATLPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2609..>2797
                     /gene="safA"
                     /locus_tag="ACHOUK_004855"
     CDS             2609..>2797
                     /gene="safA"
                     /locus_tag="ACHOUK_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFP9900110.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVILLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIVNSL"
BASE COUNT          745 a          647 c          683 g          722 t
ORIGIN      
        1 gttggcgata atccctttgt atgtactgtg catcatcgct attacaaatc ctaataattc
       61 atttccacac aggataagta gatgaagaaa aaaattgaat cctaccaggg tgctgccggt
      121 ggttggggtg ctgttaaatc cgtagcgaat gcagtacgta agcagatgga tatacgccag
      181 gatgttattg ccatgtttga catgaataag ccagagggct ttgactgtcc gggttgtgca
      241 tggccagatc ctaagcacag tgcgtcattc gacatttgtg aaaacggcgc aaaagcaatc
      301 gcctgggaag tcacggataa gcaggtaaat gcctctttct ttgctcagaa tacggttcaa
      361 tcattactta cctggggaga tcacgagctt gaggctgcgg gacgtctcac tcagcctttg
      421 aaatatgatg ctgtcagcga ctgttacaag ccattaagct ggcaacaagc tttcgacgaa
      481 attggcgcac gccttcaaag ctatagtgat cccaatcagg ttgaattcta tacttcgggc
      541 cgcacttcca atgaagctgc ctttctttat cagctttttg cccgtgaata cgggagcaat
      601 aactttcccg actgctccaa catgtgccat gaaccgacaa gcgtgggttt ggcagcgagt
      661 atcggtgtag gtaaagggac cgtgttgctg gaagactttg agaagtgcga tttagtcatt
      721 tgcattgggc ataaccctgg tacaaaccac cctcgcatgc tgacttcgtt gcgcgcttta
      781 gtgaaacggg gagcgaaaat gatcgccatc aatcctctac aggaacgtgg cctggagcga
      841 tttaccgcac cgcaaaaccc gtttgaaatg ctgacgaact ctgagactca gttggccagt
      901 gcctactata acgtgcgcat tggtggtgat atggcgttgc tcaaggggat gatgcgcctg
      961 ttaattgagc gcgatgatgc tgcaagcgcc gcaggtcggc cctcattgct ggatgacgaa
     1021 tttattcaaa cgcataccgt cggctttgac gagctacgcc gtgacgtgct caattccgag
     1081 tggaaagata tcgaacgtat ttctggacta agtcagacac aaatcgccga actggctgat
     1141 gcatatgccg ctgccgaacg aaccattatc tgttacggaa tggggatcac tcagcacgaa
     1201 catggtaccc agaatgtaca gcaactggtc aatctgctgt tgatgaaagg taacattggc
     1261 aagcctggtg cgggtatctg cccactacgt ggccactcta atgtacaggg cgaccgaacc
     1321 gtcggtatca ccgagaaacc gtctgtagag tttctggctc gcctgggtga gcgctatggc
     1381 ttcaccccac ctcatgtacc tggacatgct gcaattgcca gcatgcaagc aatatgtacg
     1441 gggcaggctc gagcattgat ctgcatgggg ggcaactttg cactggcaat gccagatcgg
     1501 gaagcgagcg ctgtaccgtt aacgcaatta gatttggcgg tacacgtagc cactaagctt
     1561 aaccgctctc atctgctgac cgcacggcat agctatattc tgccggttct gggacgtagc
     1621 gagattgaca tgcaaaaaag cggtgcgcag gcggtaaccg ttgaggattc aatgtcgatg
     1681 attcatgcct cgcgtggcgt gttaaaaccc gccggtgtaa tgctgaaatc agagtgtgct
     1741 gtggtcgcgg gaatcgcgca ggcaacacta ccccagagcg tggtagcctg ggagtatctg
     1801 gtggaagatt atgatcgcat tcgcaatgac attgaagctg tgctgccaga gttcgccgac
     1861 tataaccaac gcatccgtca tcccggtggt tttcacctga taaatgcagc tgctgaaagg
     1921 cgctggatga cgccgtcagg taaggctaat ttcattacca gcaaagggct gttagaagat
     1981 ccctcttcag cgtttaacag taagctggtc atggcgacag tacgcagcca cgatcagtac
     2041 aacacgacga tttatggtat ggatgatcgc tatcgagggg tattcggtca acgagatgtg
     2101 gtctttatga gtgctaaaca agctaaaatt tgccgtgtaa aaaacggcga aagagttaat
     2161 cttattgcgc ttacgccaga cggtaagcgc agctcacgcc gcatggatag attaaaagtg
     2221 gtcatttacc ctatggctga ccgctcactg gtgacctatt ttccagaatc gaatcacatg
     2281 ctaacacttg ataaccacga tccattaagt ggcattcctg gctataaaag tattccggtt
     2341 gaattagaac catcaaatta atgcctcttc tcatttcttc tgctgtcatc cgcacagcag
     2401 aagaattcct cattgattat tatcgcgcaa tttgctcaca tggattaaat taaactacat
     2461 actataagat ataaacttct gcctacagct gtaagaaact ccgctcagta ctgaagcacc
     2521 agtcctattt cctcttttct caagcctgtt atattaagca tactgattaa cgatttttaa
     2581 cgttatccgc taaataaaca tatttgaaat gcatgcgacc acagtgaaaa acaaaatcac
     2641 gcaaagagac aactataaag aaatcatgtc tgtaattgtg gttatcttat tactgaccct
     2701 tacgttgata gccatttttt cggcaattga tcagctgggt atttcagaaa tgggtcgcat
     2761 tgcaagagat cttacacatt tcattgtcaa tagtttg
//
LOCUS       DAZKIP010000261         2777 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZKIP010000261 DAZKIP010000000
VERSION     DAZKIP010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.263"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..546
                     /locus_tag="ACHOUK_004856"
     CDS             <1..546
                     /locus_tag="ACHOUK_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP9900111.1"
                     /translation="KTGAEAIRALAMQIPAFRQKLSDGWYLVRIAGRDTGENELSARL
                     NEPLANGAVIHIVPRLAGAKSGGVFQAVLGAALIAVAWWNPVGWLGAAAVSGMYAAGA
                     SMILGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRV
                     VSQEISTADEGDGGQVVVIGR"
     gene            607..>2777
                     /locus_tag="ACHOUK_004857"
     CDS             607..>2777
                     /locus_tag="ACHOUK_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFP9900112.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALRETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYDPEKRTYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWTDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEGIVDNGAHFDGDQSSTVNGVTPPAVQHL
                     TAEVSADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSQIELTP"
BASE COUNT          603 a          714 c          933 g          527 t
ORIGIN      
        1 aaaacggggg ccgaagccat ccgggcgctg gccatgcaga tcccggcgtt tcgtcagaag
       61 ctgagcgacg gctggtatct ggtacggatt gccgggcgtg atacaggtga aaatgaatta
      121 tctgcccgtc ttaatgagcc gctggcaaat ggtgccgtga tccacatcgt gccgcgcctg
      181 gcgggtgcta aaagtggcgg tgtgtttcag gcagtgctgg gtgcggcgct gattgcggtg
      241 gcatggtgga accctgtggg ctggctgggg gccgcggctg tatcgggtat gtatgcagca
      301 ggggccagta tgatcctggg tggtgtggcg cagatgctgg caccgaaagc cagaactccc
      361 cgtacacaga caacggataa cggcaaacaa aacacctatt tctcctcact ggataacatg
      421 gttgcccagg gcaatgttct gcccgttctg tacggtgaaa tgcgcgtggg atcacgtgtg
      481 gtttctcagg agatcagcac ggcagacgaa ggggacggtg gtcaggttgt ggtgattggt
      541 cgctgatgca aaatgtttta tgtgaaaccg cctccgggcg gttttgtcgt ttatggagca
      601 tgacgaatgg gtaaaggaag cagtaagggg cataccccgc gcgaagcgaa ggacaacctg
      661 aagtccacgc agctgctgag tgtgatcgat gccatcagcg aaggaccgat tgaaggtccg
      721 gtggatggat taaaaagcgt gctgctgaac agtacgccgg tgctggacag tgaaggtaat
      781 accaacatct ccggtgtcac ggtggtgttc cgggccggtg agcaggagca gacaccgccg
      841 gagggatttg aatcctccgg ttctgagacg gtgctgggta cggaagtgaa atacgacacg
      901 ccgatcaccc gcaccatcac gtcggcaaac atcgatcgtc tgcgctttac tttcggtgtg
      961 caggcactgc gggaaaccac ctcaaagggg gaccggaatc cgtcggaagt ccgcctgctg
     1021 gttcagatac agcgtaatgg tggctgggtg acggaaaaag acatcaccat taagggcaaa
     1081 accacgtcgc agtatctggc ctcggtggtg gtggataacc tgccgccgcg cccgtttaat
     1141 atccggatgc gcaggatgac gccggacagc accacagacc agctgcagaa caaaacgctc
     1201 tggtcgtcat acaccgaaat catcgatgtg aaacagtgct acccgaacac ggcactggtt
     1261 ggcgtgcagg tggactcgga gcagtttggc agtcagcagg tgagccgtaa ttatcatctt
     1321 cgcgggcgca ttctgcaggt gccgtcaaac tatgatccgg aaaaacgcac ttacagcggc
     1381 atctgggacg gaacgttaaa accggcatac agcaacaaca tggcctggtg tctgtgggat
     1441 atgctgaccc acccgcgcta cggcatgggg aaacgtcttg gtgcggcgga tgtggataaa
     1501 tgggcgctgt atgtcatcgg ccagtactgc gaccagtcag taccggacgg ctttggcggc
     1561 acggagccgc gcatcacctg taatgcgtac ctgaccacgc agcgcaaggc gtgggatgtg
     1621 ctcagtgatt tctgctcggc gatgcgctgt atgccggtat ggaacgggca gacgctgacg
     1681 ttcgtgcagg accggccgtc ggataaggtg tggacctata accgcagtaa tgtggtgatg
     1741 ccggatgatg gcgcgccgtt ccgctacagc tttagcgccc tgaaagaccg ccataatgcc
     1801 gttgaggtga actggactga cccggacaac ggctgggaga cggcgacaga gcttgtggag
     1861 gacacgcagg ccattgcccg ttacggtcgt aacgtcacga agatggatgc ctttggctgt
     1921 accagtcggg ggcaggcaca ccgtgccggg ctgtggctga ttaaaacgga actgctggaa
     1981 acgcagaccg tggacttcag cgtgggcgca gaagggcttc gccatgtacc gggcgatgtc
     2041 attgaaatct gtgatgatga ctatgcgggg atcagcatcg gtgggcgtgt gctggcggtg
     2101 aacagccaga cccggacgct gacgctcgac cgtgaaatca cgctgccatc ctccggcacc
     2161 acgctgataa gcctggttga cggaagtggc aatccggtca gcgtggaggt tcagtccgtc
     2221 accgacggcg tgaaggtaaa agtgagccgg gttcctgacg gtgttgctga atacagcgtg
     2281 tgggggctga agctgccgac gctgcgccag cgcctgttcc gctgcgtgag tatccgtgag
     2341 aacgacgacg gcacgtatgc catcactgcc gtgcagcatg tgccggagaa agagggcatc
     2401 gtggataacg gggcgcactt tgacggtgac cagagcagca cggtgaatgg tgtcacgccg
     2461 ccagcggtgc agcacctgac cgccgaagtc tccgcagaca gcggggaata tcaggtgctg
     2521 gcgcgatggg acacgccgaa ggtggtgaag ggtgtgagct tcctgcttcg cctgaccgtg
     2581 gcagcggatg acggcagtga gcggctggtc agcacggccc ggacgacgga aaccacatac
     2641 cgcttcacgc agctggcgct ggggaactac aggctgacag tccgggcggt aaatgcgtgg
     2701 ggacagcagg gcgatccggc gtcggtatcg ttccggattg ccgcaccggc agcgccgtca
     2761 cagattgagc tgacacc
//
LOCUS       DAZKIP010000262         2764 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZKIP010000262 DAZKIP010000000
VERSION     DAZKIP010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.264"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            117..1070
                     /gene="ompT"
                     /locus_tag="ACHOUK_004858"
     CDS             117..1070
                     /gene="ompT"
                     /locus_tag="ACHOUK_004858"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFP9900113.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETLSFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVSVNAGYYVTPNAKVYVEGTWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            1257..2741
                     /locus_tag="ACHOUK_004859"
     CDS             1257..2741
                     /locus_tag="ACHOUK_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9900114.1"
                     /translation="MRKIITHFKVVLTLLLPVTVSAQQIQWQSCMASQFNHWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDASYEKKSQVKLALTKLPAKSKHKGSILIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSTPTINCRQSDTETQENITEKQQVLNKI
                     NACIHNTGAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYN
                     VRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITQ
                     FHELLSKLHHKPLLDSKGENISSDELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDASGVILCVDQGDEQLTPEERKFRKEALANAFPAINFDNGRSD
                     SPDFCELWPIHSDLNKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVHHLITPQKIENIYCPGNSEAEIQ"
BASE COUNT          898 a          585 c          554 g          727 t
ORIGIN      
        1 aacaataatt caaacttaaa caaaccataa acagttaaac aatatacaat tgactcatta
       61 agttagatat aaaaaataca tattcaatca ttaaaacgat tgaatggaga ccttttatgc
      121 gggcgaaact tctgggaata gtcctgacaa cccctattgc gatcagctct tttgcttcta
      181 ccgagacttt atcgtttact cctgacaaca taaatgcgga cattagtctt ggaactctga
      241 gcggaaaaac aaaagagcgt gtttatctag ccgaagaagg aggccgaaaa gtcagtcaac
      301 tcgactggaa attcaataac gctgcaatta ttaaaggtgc aattaattgg gatttgatgc
      361 cccagatatc tatcggggct gctggctgga caactctcgg cagccgaggt ggcaatatgg
      421 tcgatcagga ctggatggat tccagtaacc ccggaacctg gacggatgaa agtagacacc
      481 ctgatacaca actcaattat gccaacgaat ttgatctgaa tatcaaaggc tggctcctca
      541 acgaacccaa ttaccgcctg ggactcatgg ccggatatca ggaaagccgt tatagcttta
      601 cagccagagg tggttcctat atctacagtt ctgaggaggg attcagagat gatatcggct
      661 ccttcccgaa tggagaaaga gcaatcggct acaaacaacg ttttaaaatg ccctacattg
      721 gcttgactgg aagttatcgt tatgaagatt ttgagctagg tggcacattt aaatacagcg
      781 gctgggtgga agcatctgat aacgatgagc actatgaccc aggaaaaaga atcacttatc
      841 gcagtaaagt caaagaccaa aattactatt ctgtttcagt caatgcaggt tattacgtaa
      901 cacctaacgc aaaagtttat gttgaaggca catggaatcg ggttacgaat aaaaaaggta
      961 atacttcact ttatgatcac aatgataaca cttcagacta cagcaaaaat ggtgcaggca
     1021 tagaaaacta taacttcatc actactgctg gtcttaagta caccttttaa cgaagttaac
     1081 cagattttct ccccggtggt attgtataac cccggggaat ttgtttacag ttacaagtag
     1141 acaatatcca ctaattttgt aagcatttaa cctatgtcat aacgtgtaat ctcttaccgc
     1201 aacacaagac ctcaatatat tcaaatttta tctttattcc gatattcaga gacacaatgc
     1261 gaaaaataat tactcatttc aaagttgttt taacgttact tctaccagta accgtatctg
     1321 cccagcagat acagtggcaa tcctgtatgg ccagtcaatt caaccactgg tttggtgagg
     1381 aaaaaccgtc tcctgactta ctatgtggtt atttgtctgt tccattaaaa tatacagaca
     1441 caggcggaga tgcttcttat gaaaaaaaat cacaagtcaa actagcgttg acaaaattgc
     1501 cggcaaaaag caagcataaa ggaagtatcc tgataataag tggtggtccc gggttaccag
     1561 gcataaatcc ttatattaac tttgactggc cagtcacaaa tcttcgtgag tcatgggata
     1621 ttattggatt tgatcctcga ggcgtcgggc agtccactcc gacaataaac tgccggcaat
     1681 cagatacaga gactcaggaa aacataaccg aaaagcaaca agtattaaat aaaattaatg
     1741 cctgtatcca taataccgga gccgaagtca ttcgccatat cggctctaac gaggctgtat
     1801 acgatattga tcgtattagg caagccttgg gggataaaca actgacagcc gtggcgtatt
     1861 cgtatggaac tcaaattgca gccttatatg cagaacgttt tccctacaac gtaagatcta
     1921 tcgttcttga tggagtcgtc gatatcgatg acctggagga caacttcaca tggcaactca
     1981 aacaggcaca gagttatcag gaaacgtttg atcgctttgc atcctggtgt gcgcgtacaa
     2041 aaagttgccc gctttcttca gacagagata aggcaataac tcagttccat gagctattat
     2101 caaaattaca tcacaaacct ttattagaca gtaagggaga aaatatatct tcagatgaac
     2161 tcatatcatt aacaacagac cttctgctat ggcgttcatc atggccaacc cttgcaactg
     2221 ccatacgcca gttctctcag gggattgtca gtaatgaaat tgaaactgcg ctcagtgctc
     2281 cgatagcctc agaagagtca agtgatgctt cgggggtaat cctttgtgta gatcaggggg
     2341 atgagcaatt aacaccagaa gagcgaaaat tccgaaaaga ggctcttgcg aatgccttcc
     2401 cggctattaa ctttgacaat ggacgttccg attcacctga tttttgtgaa ttatggccaa
     2461 tacatagcga cctgaacaaa actcgcctga aaaatactgt tctgccctct ggtttactgt
     2521 ttgtagcaca caaatacgac ccaacaacgc cctggattaa tgcccgtaag atggcagaga
     2581 aattttccag cccgttacta acaataaatg gtgatgggca tacattagct ctcaccggag
     2641 ttaatttatg tgtagataaa gcagttgtac atcacctgat cactccacaa aaaatagaaa
     2701 atatatactg cccaggaaat tctgaagcag aaatacaata atttcaaaat attccgctat
     2761 ttgc
//
LOCUS       DAZKIP010000263         2746 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZKIP010000263 DAZKIP010000000
VERSION     DAZKIP010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.266"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(423..599)
                     /locus_tag="ACHOUK_004860"
     CDS             complement(423..599)
                     /locus_tag="ACHOUK_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900115.1"
                     /translation="MVNKTANVAAHAVVNAALAVVQGNNARASAAGAATGDVVGMIAT
                     EMCGISPGTVTCKR"
     gene            774..977
                     /locus_tag="ACHOUK_004861"
     CDS             774..977
                     /locus_tag="ACHOUK_004861"
                     /inference="COORDINATES: protein motif:HMM:NF026057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
                     /protein_id="HFP9900116.1"
                     /translation="MVLSDCYSLANEQSGHARLGDPRRTRRLVSLTSSLAQHAGLSIV
                     KSSHFTAQVEGAYRLIRNPSVSP"
     gene            complement(909..1382)
                     /locus_tag="ACHOUK_004862"
     CDS             complement(909..1382)
                     /locus_tag="ACHOUK_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001502749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900117.1"
                     /translation="MQVMPDIRFPVTDSDYLFLYLPQPFGHALRSLLPAVTFFFFTGT
                     FFSLVLNTVGVRGTTPALLFDKIDWQGAVGGVHQDRVSQQPVRLRVSGGIPQVIKCRT
                     VTEVNGGGIFQREEQRMCLTCPCSGSKSGFGNGLRGHRRVTYQAVRTFNLSSEVG"
     gene            1302..2042
                     /locus_tag="ACHOUK_004863"
     CDS             1302..2042
                     /locus_tag="ACHOUK_004863"
                     /inference="COORDINATES: protein motif:HMM:NF033590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFP9900118.1"
                     /translation="MAERLREIQKQVITVCDREADIWHYLHYKVSRGKPHAECDAKRR
                     AGSPPGPDAHQLQRSQHKKSRQQRPGALAHVCLLRGAGEDGACWYPLTSEKVASAADA
                     RRIVSHYEQRWLIEEYHKAWKSGGTCVESLRMQTRDNLERMVVIAVRVLGLRQGGISE
                     ETQNDSCEKILTPTEWKLLWVKLEGKQLPAQTPTLKWACLKLAKLGRWHDSKRTSSPG
                     WVVMWDGWFRHQDMAEGYLVMKSLDQEI"
     gene            2276..2683
                     /locus_tag="ACHOUK_004864"
                     /pseudo
     CDS             2276..2683
                     /locus_tag="ACHOUK_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152817.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
BASE COUNT          726 a          682 c          648 g          690 t
ORIGIN      
        1 acaaaaaacc aagcatggta ataaaccgaa ctccccacaa acttctgttg cctcagatac
       61 tcccccccct ttcctcaggc acaaatgttg ttccataacg aaccagccct gttcctttaa
      121 actctgcatc aatggcataa tgtatactca ccagactgag tttgctcctg catcattatt
      181 tcgatacgtt aatgcattgt tcattgcatc cggcattccc catcgaatat ttactttgtt
      241 atatcagtca gcccataacc aggtaaattt ttaaggctta ctgactgaat attcaaaatg
      301 tgttacatgt tatcaaacct ttaaagaata acctgctctt cttcattggc cggatttagc
      361 tcaatcttgt cagcaaaact aatataaagc cgttcataaa gagaaggatg tagcttcgtt
      421 atttatcttt tacatgtaac agtgccaggg gatattccgc acatctctgt ggctatcatc
      481 cccaccacgt ctccagtggc agcacctgcg gcactggcac gggcgttatt tccctgtacc
      541 accgccagtg cagcattcac cacggcatga gctgccacat ttgcagtctt attcaccaca
      601 cctgtaacct gtctcttata cacaaatccc cacccgggga tttgtgatgt ctgtcagtct
      661 gctttagggg gactctttcc atccggtagt ttccttcctc cccggcagcg attttttgaa
      721 ctttttcttc acccgttgat cttctcttat atttcatttt cttcgggtct gttatggtac
      781 tttctgattg ttattcactg gctaacgagc agtctggaca tgcccgtctc ggtgatccgc
      841 gtcgtacacg acgccttgtg tcactgacct cttctctcgc tcagcatgca ggtctttcta
      901 ttgtgaaatc atcccacttc actgctcagg ttgaaggtgc gtaccgcctg atacgtaacc
      961 cgtcggtgtc cccgtaagcc attgccgaag ccggatttac tgccactgca agggcatgtg
     1021 aggcacatcc gctgctcctc gcgctggaag ataccaccac cattaacttc ggtcacagta
     1081 cggcatttga tgacctgggg aataccacca ctaacccgaa gacgcacggg ctgctggctc
     1141 actctgtcct gatgtacgcc cccgacagcg ccctgccagt cgatcttatc gaacagcagt
     1201 gctggagtcg tgccacggac acctacggtg ttaagcacca gcgaaaagaa cgtccctgtg
     1261 aagaaaaaga aagttaccgc tggcagcagg cttcggagcg catggccgaa cggctgaggg
     1321 agatacagaa acaggtaatc actgtctgtg accgggaagc ggatatctgg cattacctgc
     1381 attataaagt cagccgcggg aagccacacg ctgaatgtga tgcaaaaagg cgggcgggca
     1441 gcccgccagg cccggatgct catcagttac agcgaagtca gcataaaaaa tcccgacaac
     1501 agcggccagg cgctctcgct cacgtatgtc tgttgcgggg agcaggagag gacggagcct
     1561 gctggtatcc gctgacatca gaaaaagtgg cgagtgccgc cgatgcgcga cgtatcgtca
     1621 gccattacga gcaacgctgg ctgatagagg aataccacaa agcgtggaaa agtggtggta
     1681 catgtgtgga atcgctgcga atgcagaccc gggataacct tgagcgcatg gtggttatcg
     1741 cggtgcgggt gctgggcctg cggcagggag gtatcagtga agaaacacaa aatgacagct
     1801 gtgagaaaat actgacgccg acagagtgga aactactgtg ggttaagctg gaaggaaagc
     1861 agcttccggc gcaaacaccg accctgaaat gggcctgttt gaaactggct aaactggggc
     1921 gatggcatga cagcaaacgc acaagcagcc ccggttgggt agttatgtgg gatggctggt
     1981 tcagacatca ggatatggct gagggttacc tggtgatgaa gtctcttgat caggagatct
     2041 gatcaagaga caggtcaaag atgggggaca acgtctggtg ctgtataaca gcacaggcct
     2101 cacactcttg acgcccgtgg aatatattag agaacaaaaa tttcaatatg tggtggacgc
     2161 atacagagga ttgacagcat tcccatacta attgttaaga ttcaaattga agtatatcta
     2221 tctgtcattt tgcaaatgat atgcggtagc tttacctaac tatcatgaac atttcgaaaa
     2281 atcatccaca aagccccgaa gaataaagca ctatcgcccg ataaagacct gtaagctcag
     2341 tggcatggat actattgagc tcagaatggc gagctgtacc gttatttcat taccatgctc
     2401 gacgaacaca gcaactactc tctggccgta ctatcgctta aaagtgatat catcaggcat
     2461 ttcttctgct gtgtagccca gttgttcctt gttggtatca accagatcat cactgatgat
     2521 gggcaaagag ttctcgggaa aattcgacaa aacgctacag gaagccaccg tcgggttcgt
     2581 atggacttat ctctatacgc cgaaaataaa tgccataagt gagcatttta accgaacact
     2641 gagggaacaa tttattgagt ttaatgaaga attgctcttt gaatagctga atatgttcaa
     2701 tctgaaactg gctgagtatc tggtactgta caacagtaaa attctt
//
LOCUS       DAZKIP010000264         2697 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZKIP010000264 DAZKIP010000000
VERSION     DAZKIP010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.267"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..493
                     /locus_tag="ACHOUK_004865"
     CDS             <1..493
                     /locus_tag="ACHOUK_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFP9900119.1"
                     /translation="QPKKTTLRDKAMVNYAFDYLSSPGSLPFTTAATELSAIHGHSTS
                     QYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRAQSKLGWIYLKGLGVKPDTRKA
                     ILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQKWLKLAAKQHYKNAERLLAGL
                     PAH"
     gene            complement(503..1930)
                     /locus_tag="ACHOUK_004866"
     CDS             complement(503..1930)
                     /locus_tag="ACHOUK_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFP9900120.1"
                     /translation="MKNCWKILNIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMNEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQIILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWHEYPSTQADALMLQWCRQHRVDYYPLVVMMIEARELVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGCKPRISLTRDTEHTLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAENRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGIIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(1927..2634)
                     /locus_tag="ACHOUK_004867"
     CDS             complement(1927..2634)
                     /locus_tag="ACHOUK_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFP9900121.1"
                     /translation="MDMESQKILFALSTPMEIRNECCLPSHSSPKMYLGTRFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDNLPLLREFLARKEYDDSGNDMFYQLFASDDAYYATLPWQP
                     LADYPTCPETLKDMSDL"
BASE COUNT          759 a          643 c          600 g          695 t
ORIGIN      
        1 ccaacccaag aaaacgacgt tgcgtgataa agccatggtg aattatgcct ttgattattt
       61 aagctcaccg ggcagtcttc cattcaccac ggcagccacg gagctttccg cgattcatgg
      121 tcactcaacg tcgcaatatc gccttggaga attttatctt catggtagcg acggtaaacc
      181 actggattat acacaggcga gatactggta tgagcaatca gcggaacagg aaaatccacg
      241 cgcgcaaagt aaactggggt ggatctacct caaaggtctg ggggtcaaac ccgacacccg
      301 taaagcaatt ctctggtata aggaagcagc tgaacaaggg tatgctcatg ctcaatatac
      361 tttaggtttg atctacagaa atggctcagg tattaatgtt aaccattatg aatctcaaaa
      421 atggttaaaa ctggccgcca aacaacatta caaaaatgcg gaaagattac ttgccgggct
      481 tcccgcacat taacgtcgac tgtcacccga acagatggag tattttccct atcaaaccag
      541 caaagataaa aatatagaaa ataattttaa tgatgcccag acagcctggc ccaccatcgt
      601 caacgggcat tggctgcggt tgcggctccg gttcaacagt ttgcttctcg gtaaaattcg
      661 ccgcctgaat taataatcga cgaataatta atgcttccag tggacaacga ttttctgcat
      721 caagacggct tacgatcggc attaatgatt cttctgtagg ttggctggcc tgcactaatt
      781 gtttggcaac tagatataat ggccataagg tatgttctgt atcacgcgtg agtgagatcc
      841 gcggcttaca tcctttgtgc aaaaccatct cgaccagtga acgccccaga gcagagagtt
      901 tttcatcgct gagtatttcg tataaatgaa aacgcgtacg ggcgctgtcg ccggggacat
      961 aaagtagcgg ttttccctgg tcgttaacca gttcacgagc ttcaatcatc atcaccacta
     1021 atgggtaata atcgactcga tgctgacggc accattgcaa cattaaagca tccgcctgag
     1081 tggagggata ctcgtgccag tagtcacaaa gttctgccag caggctttcg ccttctccac
     1141 aatagacgcg ttgcgcgtat tcataataac gcgcatcttc attatcaggt tcagcttccc
     1201 gccacgaccg ggcgtaatcc aggagttcgg ggattcccag gcgacctgca ctataccagc
     1261 ggagtaaatt tctgtgcagt tttgcatcat cggggataat cactgcgcga tgttgcgcta
     1321 aatacaatgt cagccattct ggatgcgatg accaaatccg ggcgaacatt tggtaaaaat
     1381 cgatggtgtc attttgcacg gcaacgggca gatgcagaat gatctggaag ttaaaaacat
     1441 ttcccttcgc aatggcatat aaaaaggatt ccagttcttc ttcgtctatt tccccgacgt
     1501 tttcctcctg caaccaccgc aatctttctg ctaataacac cacgcactcg aaggataaat
     1561 gggcagtgtt catggctatt gtgcacagcg accagcgtaa ttcatcaatc tcattcatcg
     1621 agcaataatt taattgctga atgaatcgct gccaggcgga gggcagaaaa cgttcactat
     1681 cagaggctag taacgcacga aaggcgagca gaatttcatg ttctgctacc tcatattctt
     1741 ctggttgcca aacagattta gcaggcgact gcgcaatccg gagcgcttcc tcatacgcct
     1801 gacgaagttg tttaaaaccc tgcggatcgg tttctggatg aaaggacggt aacagcgcca
     1861 gataagcgcg gcggataata tcgacatcag tcgtttcctc tatgttgagg atcttccagc
     1921 aatttttcat aggtcgctca tatccttaag cgtttccggg catgtggggt agtcagccag
     1981 tggttgccag ggtaaggtcg cgtaatatgc atcgtcactg gcaaataatt gataaaacat
     2041 atcattgccg gaatcgtcgt attctttacg cgctaagaat tcacgtaata aaggcagatt
     2101 atcatcttcg ggagactgcc aatattgccg accaaaggta taaccggcaa agtattgccg
     2161 ccagttacta taataacgta gagcccgtag atgaatacgc gactggatcc atagactttc
     2221 ttcttcactc aaccagttat taagtacgcc cattcgactt aaaaaaccca tccggacata
     2281 atcccatgct ttaattcctc cctcaccaca gcattcggct gtgctggcga caaattgcgc
     2341 ataagcctga ccttgctcat ttaattcagc aagatagtca cgccattcac atggagaata
     2401 acgaaaccag cggtgataaa accctgccaa ccgcgcggca tgaccgttat cgatcattcg
     2461 gtgaattgtg cgcagcaggt catcacggtc atcaattccc caggaggaag aaaggtcaaa
     2521 aaagcgtgta cctaaataca ttttgggtga tgaatgcgaa ggtaaacagc attcattgcg
     2581 tatttccatc ggagtggaaa gtgcaaacaa tattttttgc gactccatgt ccatatgatt
     2641 tatccttaaa tattcattga atgaatatta gccgcattgc gacgctcgtt tatttat
//
LOCUS       DAZKIP010000265         2674 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZKIP010000265 DAZKIP010000000
VERSION     DAZKIP010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.268"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            20..238
                     /locus_tag="ACHOUK_004868"
     CDS             20..238
                     /locus_tag="ACHOUK_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFP9900122.1"
                     /translation="MKHYLEKNYPRKSRTTEFLFFILFIVLMIPISPLLLVWIIGRTF
                     EPVIELYTDVTWESFSALHNKINPYKEN"
     gene            240..797
                     /locus_tag="ACHOUK_004869"
     CDS             240..797
                     /locus_tag="ACHOUK_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFP9900123.1"
                     /translation="MSTITRERAEIKSYITGFLSDSAHDNKSSDSLLANVFRIALASL
                     EAEPIAMVVPDEMDLLTCHLDGVTKTYADGWNACRVAMLQAGNFRENKNSSTNNFREI
                     SETSTRSPITLDGWISCTERMPEKSQNVLISMNIDSEAGPLIYSARYLGGTFRRGGIA
                     VSPGNDLRQATHWMSLPEPPQEVNQ"
     gene            1088..1243
                     /locus_tag="ACHOUK_004870"
     CDS             1088..1243
                     /locus_tag="ACHOUK_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP9900124.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESEK"
     gene            1363..1707
                     /locus_tag="ACHOUK_004871"
     CDS             1363..1707
                     /locus_tag="ACHOUK_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiW/YdeI family stress tolerance OB fold
                     protein"
                     /protein_id="HFP9900125.1"
                     /translation="MKKVLIAALISGVSFGAFAQQGGFQGPEAERSTVAQAKELKDDA
                     WVILEGSIVKKVGDERYEFRDNSGTIVTDIDDSVWAGQNVSPKDKVRIEGEIDKDLSS
                     VEVDVKALKLLK"
     gene            1829..2101
                     /locus_tag="ACHOUK_004872"
     CDS             1829..2101
                     /locus_tag="ACHOUK_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900126.1"
                     /translation="MTQHIKSHNSEADPEIKQGRRFRAPQYGWFHYLFCTIDEADMLQ
                     EAYLRRGVRVERSLNADRLTWTVSVYLPVRAHLPRTHACYRQRVWR"
     gene            2103..>2674
                     /locus_tag="ACHOUK_004873"
     CDS             2103..>2674
                     /locus_tag="ACHOUK_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP9900127.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESLPVFHRGRVLVEPEPKNMRA
                     LPSGAVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHSYGAGAMVLCWHCDNQLRDQTSESLEQLTQQNLTAWMIDVIRHVMNGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRRSLGLP"
BASE COUNT          695 a          608 c          726 g          645 t
ORIGIN      
        1 cccgaaaggg cggtaagaaa tgaaacatta tttagaaaaa aattacccac gaaagagcag
       61 aacaacagag tttctgtttt tcattctgtt tatagtgttg atgataccga tatccccgct
      121 attactggtc tggataattg gaaggacatt tgaaccagtt attgagctat ataccgatgt
      181 gacatgggaa tcattcagcg cactgcacaa taaaattaat ccgtataagg aaaactgata
      241 tgagcactat taccagagaa cgcgcggaga ttaaatcata catcacaggc ttcctgagcg
      301 actcggcgca cgataacaag tcttcagaca gcctgctggc taatgtgttt cgtatcgcgc
      361 tggcatcact ggaagcagag ccgatagcaa tggtagtgcc tgatgaaatg gatttgctta
      421 cctgccatct cgacggtgta actaaaacat atgctgatgg ctggaacgcc tgccgcgtcg
      481 ccatgcttca ggccggaaac tttcgggaaa ataagaattc gtcaaccaac aattttcggg
      541 aaatctcgga aacgtcaacc agatctccga taactctgga tggctggata agctgtactg
      601 agcgaatgcc tgaaaagagc cagaacgtgc ttatttcgat gaatatcgat agcgaggctg
      661 ggccattaat atattccgca cgctatctcg gaggcacgtt ccggcgcgga ggtatagcag
      721 ttagtccggg taatgatctt aggcaagcaa cccactggat gtcgctacca gaaccgccgc
      781 aggaggtgaa tcaatgacct ggcctgaagc attcacaacg gtaggaattg cgatggcggt
      841 ggcgctggtg gtgtattcga tttgccgctg gggataaaaa cggtttgcgg gaaaaggata
      901 gttaagtaga attgctgcgg gtgcttgagg ctatctgcct cgggcatgaa caccaacggc
      961 agatagagaa aagccccagt taacattacg cgtcctgcaa gacgcttaac attaatctga
     1021 ggctcaatcc atgctgaaca catgtaggtt agcctcttac gtgccgaaag gcaaggagaa
     1081 gcaggctatg aagcagcaaa aggcgatgtt aatcgccctg atcgtcatct gtttaaccgt
     1141 catagtgacg gcactggtaa cgaggaaaga cctctgcgag gtgcgaatcc gaaccggtca
     1201 gacggaggtc gctgtcttcg tagactacga atctgagaag taagagacca ggcgggggag
     1261 taatctcccg ccacctctga tgtgtcaggc atcctcaacg cacccgcgct ttaccatact
     1321 gaaaatgctg tttgaatgtt catctctgaa agaggactat gaatgaaaaa ggtattgatt
     1381 gcagcactta tttccggtgt gtcttttggc gcttttgcac agcagggtgg tttccagggg
     1441 ccagaagcag agcgttcaac agtagcgcag gcaaaagaac tgaaggatga tgcatgggtt
     1501 atccttgaag ggagcatcgt taaaaaagtg ggtgatgaac gttatgagtt tcgtgacaat
     1561 agcgggacaa ttgtcacgga tattgatgac agcgtatggg ccgggcagaa tgtttctccg
     1621 aaagacaaag taagaattga gggtgaaatt gataaagacc tgagcagtgt tgaagttgat
     1681 gtaaaggcac tgaaattatt aaagtaaccg cccctgcttg ttaagcccgt cttactgacg
     1741 ggttttctgt ttgtacattc cggcgtattg ccttacaatt cgcgcagtca gcctgaacaa
     1801 ctgacacctg ctgtcaccgg agaatccgat gacacaacac ataaaatccc acaattctga
     1861 agccgacccg gaaattaagc aggggaggcg ttttcgtgcg cctcagtatg gctggtttca
     1921 ctatctgttc tgtacgatcg atgaggcaga tatgcttcaa gaggcgtatc tgcgtcgcgg
     1981 tgtccgtgtg gagcggagtc tgaacgctga tcgtctgacc tggaccgttt ctgtatatct
     2041 tcctgttcgt gcacatctgc cacggacaca tgcctgctac cgtcagcgcg tctggaggta
     2101 atgtgcgggt attacttcga cctgttctgg ttccggaact cgggctggtg gtccttaagc
     2161 ccggtcgtga atcattgcca gtttttcatc gcggcagggt gctggtggag ccggaaccga
     2221 aaaacatgcg ggcgctgcca tctggagcgg ttcctgctgt tcgccagccg ctggcggaag
     2281 ataaatcact gctgccattt ttcagcgatg agcgggtgat tcgtgcagct ggcggcgctg
     2341 gtgcactgtc tgactggtta ttacgtcacg tgaaatcctg ccagtggcca cacggcgatt
     2401 atcatcacag cgaaaccgtt attcacagtt acggtgctgg cgcaatggtg ttgtgctggc
     2461 actgcgacaa ccagctgcgc gaccagacct ccgaatcact tgagcaactt actcaacaaa
     2521 atctgacagc ctggatgatt gacgtcatac gccatgtaat gaatggcacg caggagcggg
     2581 aattatcgct ggctgaatta tcctggtggg cagtctgcaa tcaggtggtg gacgcattac
     2641 ctgaggcagt atcgcgtcgc tctctgggat tacc
//
LOCUS       DAZKIP010000266         2625 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZKIP010000266 DAZKIP010000000
VERSION     DAZKIP010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.269"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..338
                     /locus_tag="ACHOUK_004874"
     CDS             <1..338
                     /locus_tag="ACHOUK_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900128.1"
                     /translation="GMFGKGTRAVMHAIFTICFAEFLSRKGNPFIGFVVLDSPLVTHF
                     DKDRGGSLSDVNSVSLSDSFYHALIKRDYNFQIVILENKGPTFQIKINDANKIHNLNK
                     NGSSGFYPV"
     gene            complement(748..1020)
                     /locus_tag="ACHOUK_004875"
     CDS             complement(748..1020)
                     /locus_tag="ACHOUK_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFP9900129.1"
                     /translation="MALKCPECGTTAHARTSAYEAPSVKRSWYQCQNLECSYTFTALE
                     SVDTIIMKPRRNEQESEKAQMPEKQQQTLNRYGSASKLSSRQQIPV"
     gene            complement(1026..1577)
                     /locus_tag="ACHOUK_004876"
     CDS             complement(1026..1577)
                     /locus_tag="ACHOUK_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage polarity suppression protein"
                     /protein_id="HFP9900130.1"
                     /translation="MMARVTPDQALISFRNARILWAGHSESRKAVEQQIESLLTAMEK
                     PADYARQLELQREHLDVLKWQINCAARECIYSQHQLMEACTEDALSNFMQANGAALTS
                     ALAPFLKGRGGVDVASRILRNALVRQLAITPPEIAGDYRMILDESGVMPDPMMVRDCQ
                     SSYTPAQQLRFQQRLDYINGMQE"
     gene            complement(1574..2326)
                     /locus_tag="ACHOUK_004877"
     CDS             complement(1574..2326)
                     /locus_tag="ACHOUK_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation initiation protein"
                     /protein_id="HFP9900131.1"
                     /translation="MTIPEQNNNPIVEISIPVSTQQALEKVNATKSAWLEARRQQKAA
                     ADNIATIRQRRAEMEATTNALNEEWRTLFRESQGVVSKEMKKLRTEIALGRETLEDFD
                     ELLAAQESENALLPQEAAELAGKYIHAHDTLVGIRAKQIWEDFMQSHGKALIQTLSLL
                     KSTMGREASAVVGVVHSVNDPDTLLKDFIHKHITRPALTNDAMPEQDPVFKLAGVAPD
                     YAARLDFSNKLSPAAMHKMKVRQERAEKEKAV"
BASE COUNT          632 a          611 c          608 g          774 t
ORIGIN      
        1 gaggaatgtt tggtaaaggt acccgtgcag taatgcatgc tatatttacc atttgctttg
       61 cggaattttt atcaagaaaa gggaatccat tcattggatt tgttgtgctt gatagtccat
      121 tagtgacgca ctttgataaa gatagagggg gttctctatc ggatgttaat agcgtcagtc
      181 tcagtgattc attttatcac gcattaatca aaagggacta taatttccag attgtaattc
      241 ttgagaataa aggtccgacg ttccaaataa aaattaatga tgcaaataaa atccataact
      301 taaataagaa tggaagctct ggtttttatc ctgtttaata cattaaaaat ataactgcta
      361 attggggtgc tcgaaattat atttcgtttc gagcgctcag ttcatttcta aataatttgc
      421 aaaattattt tgggtacttt cgcctctaca cttccttcgg gtggttccac aataatggat
      481 ctcaccggcg tttgatattt tataatctaa ccccttagcg cgcgcagccc cccacgcctg
      541 ctcgcttcgc ttaacagact ggttttcatg cattccgcaa atcgtctcag aagccaccac
      601 acaagggctt tcgcgtcaaa aatggtgcat gagaatcatg cgttttcatg cgctatagac
      661 atgcactcat gcgctctcag gccagccagg gaaaaggcgt aaaaaatccc agtactggac
      721 cgggattacg tgggcttttt ttgctaatca gacaggaatt tgctgacggc ttgacagctt
      781 tgacgcggag ccatagcgat tgagagtttg ctgttgtttt tccggcattt gtgctttctc
      841 tgattcctgt tcattgcgcc ggggcttcat gattatcgta tccacacttt ccagtgcggt
      901 aaatgtgtag gaacattcaa gattctggca ctgataccat gagcgtttaa ccgatggtgc
      961 ttcataggcg gaggttctgg cgtgtgccgt cgtaccgcat tcgggacatt taagtgccat
     1021 taatattact cctgcatgcc gttaatgtaa tccagacgtt gctgaaagcg cagttgttgt
     1081 gcaggggtgt aagagctctg gcaatcacga accatcatgg ggtcaggcat cacaccggat
     1141 tcatccagaa tcatacgata atccccggca atttcaggcg gtgttattgc cagctggcga
     1201 accagagcat tgcgtaatat acgggacgca acgtcaaccc ctccgcgccc cttcaggaac
     1261 ggagccagtg cagatgtcag ggctgctcca ttcgcctgca taaaattgct gagagcgtct
     1321 tctgtgcagg cctccatcag ttgatgctgc gagtaaatgc attcgcgcgc agcacagtta
     1381 atttgccatt tcagcacatc cagatgttcc cgctgtaatt cgagctgtcg agcataatct
     1441 gctggttttt ccatggcagt gaggagtgat tctatctgtt gttcaacggc ttttcggctc
     1501 tcactatgtc cagcccacag aatacgggca tttctaaaac tgataagcgc ttggtctgga
     1561 gtgactcggg ccatcatact gctttctcct tctcagcacg ttcctgacga accttcattt
     1621 tatgcatagc tgctggagag agtttattac tgaaatccag acgggccgca taatccgggg
     1681 caactcccgc cagtttaaac acggggtcct gttcgggcat cgcatcgtta gtcagagccg
     1741 gtctggtgat atgtttatga ataaaatctt tcagcagtgt gtccgggtca ttaactgaat
     1801 gcaccacacc gacaactgcg ctggcttccc tgcccattgt tgatttgagc aggcttagtg
     1861 tctggattag tgcttttcca tgcgactgca taaaatcttc ccagatttgt tttgcgcgaa
     1921 tacccacaag ggtgtcgtgc gcgtggatat actttccggc taattccgca gcctcctgcg
     1981 gcaaaagtgc attttcgctt tcctgagccg ccagcagctc atcaaaatcc tcaagcgttt
     2041 ctcgtcccag cgcaatttcc gtgcgcagtt ttttcatttc ctttgagact acgccctgac
     2101 tttcacgaaa tagcgtgcgc cactcctcat tcagggcgtt cgtcgtggct tccatttcag
     2161 cgcggcgctg gcggattgtc gcaatattat ccgctgccgc tttttgctga cgacgtgctt
     2221 caagccatgc agatttggtg gcattcactt tctcaagcgc ctgttgcgtc gaaacaggga
     2281 tagaaatttc aacgatcggg ttattgttct gttctgggat tgtcatgtca gcacctagtg
     2341 gtttgtcgtg gtgtcaattg tgctgtgatg catacaaagc tgccattgaa cgccattgtg
     2401 cgagcgacaa gacaaagact gaaccgcccc ggacctcttt tggctagcca gaaaaggcct
     2461 cgtttaacta aggctgtttc agacttacct taactattca ctactattca cttaggtaaa
     2521 aaaaataggt aatacagaag gttataaggt gaagggttgg catggcagta ttcatcgact
     2581 gttcataact gttcacatgg gaataaaatt atttatggta ttttt
//
LOCUS       DAZKIP010000267         2605 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZKIP010000267 DAZKIP010000000
VERSION     DAZKIP010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.270"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            365..508
                     /locus_tag="ACHOUK_004878"
     CDS             365..508
                     /locus_tag="ACHOUK_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFP9900132.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            521..730
                     /gene="cspE"
                     /locus_tag="ACHOUK_004879"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             521..730
                     /gene="cspE"
                     /locus_tag="ACHOUK_004879"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFP9900133.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            896..1705
                     /gene="rlmA"
                     /locus_tag="ACHOUK_004880"
     CDS             896..1705
                     /gene="rlmA"
                     /locus_tag="ACHOUK_004880"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFP9900134.1"
                     /translation="MSFSCPLCHQPLTREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLNEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQKSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQILAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1702..2268)
                     /gene="mntP"
                     /locus_tag="ACHOUK_004881"
     CDS             complement(1702..2268)
                     /gene="mntP"
                     /locus_tag="ACHOUK_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFP9900135.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(2265..2387)
                     /locus_tag="ACHOUK_004882"
     CDS             complement(2265..2387)
                     /locus_tag="ACHOUK_004882"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900136.1"
                     /translation="MHINRYACMNAKTHRGLLFMYACLLGAMMCFMDTPVRTLS"
BASE COUNT          699 a          604 c          623 g          679 t
ORIGIN      
        1 ttctctttgg ggtgattgag agccgttaac tgcctgtttt atgagaaaaa gtgatataac
       61 tttttattca ttgcatagca gaaaatgtga tattgcacgc actatgtaat aacttctccc
      121 actgacctgg aacaactgaa cttattgaac tatgttagaa actacgtcag tttaagtatc
      181 tgcggaactg acaaggttaa gcacaatgat atatcggcgc gtattccgtt gcataagtgt
      241 gcaaaaaaag tggaagacgt atcgagattt gtgcgtctga tcgagacatg tttaaaaatg
      301 gcttgccata attaacgttg tatgtgataa cagatttcgg gttaaacgag gtacagttct
      361 gtttatgtgt ggcattttca gtaaagaagt cctgagtaaa cacgttgacg ttgaataccg
      421 cttctctgcc gagccttata ttggtgcctc atgcagtaat gtgtcagttt tatctatgtt
      481 atgcctgcgg gcgaagaaaa caatctaagg aatttttcaa atggcaaaga ttaaaggtca
      541 ggttaagtgg ttcaacgagt ctaaaggttt tggcttcatt actcctgctg atggcagcaa
      601 agacgtgttc gtacacttct ccgctatcca gggtaatggc ttcaaaactc tggctgaagg
      661 tcagaacgtt gagttcgaaa ttcaggacgg ccagaaaggt ccggcagctg ttaacgtaac
      721 agctatctga tcgaatccac tgatctgaag tgtgaatacg cttcaatctc gctataaagc
      781 ctcgtcgaat gcgaggcttt ttactatgct ttatcttcgc tcctggcgtt cggatatttg
      841 cccgccgcgt gattcgcgtt acacttgcgg cctttagtat cctgccggag ttgtcatgtc
      901 gttttcctgt ccactttgcc atcagcctct tacgcgtgaa aaaaacagct atatctgtcc
      961 ccaacgacat cagtttgata tggcgaaaga agggtatgtc aatctgctgc ccgttcagca
     1021 taaacggtct cgtgatccgg gcgacagcgc ggaaatgatg caagcacgcc gcgcattctt
     1081 agatgccgga cattatcagc cgctgcgtga tgcaattgtc gcccaactgc gggaacggct
     1141 taatgaaaag gccacggcgg tgctggatat tggctgtggt gaagggtatt acacacacgc
     1201 atttgccgat gcgttgcccg aaatcaccac gtttggtctg gatgtttcga aggtagcgat
     1261 aaaagcggcg gcgaaacgct atccgcaggt cactctttgt gtcgcttcca gccaccgttt
     1321 gccgttttcc gataccagta tggacgccat aatacgtatt tacgcgccgt gtaaagcaga
     1381 agaattagca cgagtagtga agcccggcgg ctgggtcatt actgccacgc cgggaccgcg
     1441 acatttgatg gagctgaagg ggctgattta caatgaagta catcttcatg cacctcatgc
     1501 agaacaactg gaaggtttta cattacagaa gagtgatgag ttgtgttatc cgatgcgtct
     1561 tcgcggtgat gaagccgtcg cattattgca gatgacgccg tttgcctggc gtgcgaagcc
     1621 agaagtctgg caaatactgg cagcaaaaga agtgttcgac tgccagacgg actttaatat
     1681 tcacctctgg cagcgttctt attaaccgtg gaagtgcgtc cagaggatct ggacgccgat
     1741 gccgatcagc accagcccgc cgagaatttc cgcttttttc ccaataattg agccgataaa
     1801 gcgaccaacc atcatcccta atgtcgacat aatcaaggtt gcacaaccaa tggccaatgc
     1861 ggtcgcgata atgttgacct gaaggaaagc aagaccaaca cccacagcca tggcatccag
     1921 gctggtggca atcgcggtgg ttaccagtag ccagaaaccg tgccgacggc gcggctcttc
     1981 atcttcatca tctgcgccac gaaaaccctc aataatcatt cgcccgccga ggaatatcag
     2041 cagcacaaac gcaatccagt gattccattc aaggacaaac cggctggcta acatgcccat
     2101 tccccagccg atcagcggcg tcagggtttc gacggcacca aaaataaggc cggttcgcaa
     2161 tgcttcagaa aatttcggtt tatggagggt ggcaccttta ccgattgatg cagcaaatgc
     2221 atccatcgac ataccaaacg caagaagaac agtagcagtg atattcatga caacgtcctg
     2281 accggggtat ccataaaaca catcatcgcc cccagcaaac atgcatacat gaacaacaag
     2341 ccgcgatggg ttttcgcgtt catgcaggca tagcgattga tgtgcatatg gtctcgcctg
     2401 actgaaagtc ttcagtccgt acgtgccacg ttttgcaacg agtatgttga cacgtacatt
     2461 tccggaatct ggaaattggc tactccccaa atgagggcgc aaccttaaca tattttgaga
     2521 atataaaaca actgttggaa tatattattt tattgctttg ttgataacga ttttcattta
     2581 gatttatata tcaatgaaac gttat
//
LOCUS       DAZKIP010000268         2549 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZKIP010000268 DAZKIP010000000
VERSION     DAZKIP010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.271"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(125..199)
                     /locus_tag="ACHOUK_004883"
                     /pseudo
     CDS             complement(125..199)
                     /locus_tag="ACHOUK_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(196..621)
                     /locus_tag="ACHOUK_004884"
     CDS             complement(196..621)
                     /locus_tag="ACHOUK_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9900137.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGASVARIAREHDIND
                     NLLFKWLRLWQNEGRISRRLQVTTSSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQ
                     TSVSAGSCKVEFRHGNMTLENPSPELLTLLIRELTGRGR"
     gene            708..923
                     /locus_tag="ACHOUK_004885"
                     /pseudo
     CDS             708..923
                     /locus_tag="ACHOUK_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(966..1305)
                     /locus_tag="ACHOUK_004886"
                     /pseudo
     CDS             complement(966..1305)
                     /locus_tag="ACHOUK_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347735.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(1621..2265)
                     /locus_tag="ACHOUK_004887"
     CDS             complement(1621..2265)
                     /locus_tag="ACHOUK_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900138.1"
                     /translation="MMKQGMANSISMTWQLLCDEHGFTREHYNQLKKFSPETLREIIA
                     EIASCHPSTSVLLRNKWLTPPEDILEQITREYERRIQNCPPFRSEKEAESWLNELSFA
                     VIAPLYRVAPEQTEQVESFVLQLIAEQERVWELILTNDGYSWHCALYDILFHLVLRNM
                     EDQPEHQKQRITKIFYEPDARLVAEEIKFRIQSLYDDEQKRALSELVNDFTSKE"
     gene            complement(2286..>2549)
                     /locus_tag="ACHOUK_004888"
     CDS             complement(2286..>2549)
                     /locus_tag="ACHOUK_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP9900139.1"
                     /translation="AQKLEAAGCWRRASDRWLFVMGNVECTEAQREWLLLRRNYCLAQ
                     ISSPPLPETLDISEVAKAADATLRRMGIATPSGEVFRKGTPVC"
BASE COUNT          651 a          635 c          553 g          710 t
ORIGIN      
        1 ggaaacatat aataattttc atcaaaatag tatttatgta ctaactaagc tggatttgaa
       61 cctctatttc tgggtatttg ttatcactta atccattact gctcctcctt cagcatatgt
      121 cgtatccgtt gaaggattta cgcatatcgg taacgtcggc aacgagccag atgcgggtac
      181 ctgacgggag tgagatcatc gtcccctccc ggtcagttca cggatcaaca gcgtgagcag
      241 ctctggcgat ggattttcca gcgtcatgtt accgtgacgg aactccacct tgcaggagcc
      301 ggcactgaca ctggtctgag tggaagtgga taaagacggc gcaatggccg ccaccggttc
      361 tttctgctca tccggcgtta tttctacagg taataattca acgccagtgt cagaagaggt
      421 cgttacctga agacgccgtg atatacgccc ttcgttctgc cagagcctga gccatttgaa
      481 caggaggtta tcattgatat cgtgttcccg ggcaatacgg gcaacagagg ctcctggttg
      541 tgatgccagt tccaccatac gaagtttgaa ttcattcgaa tagtttttac gaggttcttt
      601 tcgccagtcc tgtaattcca tacttagatg tccgtctata tcagatgggc gtctaagtta
      661 ccaattttcg tctgatggct acatacggcg gtcagtttac gcttactgac aacgcgatgg
      721 cggagagcat caatggtctt tacaaagcgg aggtaataca ccgtaagagc tggaaaaacc
      781 gtgcagaagt ggaactggcc acactcacgt gggtggactg gtataacaat cgacgattgc
      841 tggaaaggct gggccatact cctccggcag aagcagaaaa agcttattat gcttccatcg
      901 gaaacgatga tctggcagcc tgagttcaca gataaaacac tctccaggaa acccggggcg
      961 gttcaaacct gattctggaa aaggactttg tggattgttg ctattgccag tgagattgaa
     1021 tgaagtcgtt ttaactcgct ttgaatacgt cttgccccca gatttcgctg agaacgtaac
     1081 tccagaatca gagcgacttc gcaagtgccg gttttcgttg atggtgaacg cttggggcga
     1141 cggctgtggc tttccagacc agcgatgccc tgagccagat atctgcgcca ccattttacg
     1201 caatgtgggc cgagaaatgc cacagcgacg gcatacaaat cccgcatcac cggacgtttc
     1261 atagagtttt acccactgta gccgctgctg aatttccctg ttcataattt ggcattagtt
     1321 cagctcaatc ccgacttaaa aagtccctac aggatgttcc ggacctgagg cacagttcat
     1381 atccttatcc ataaaaaatt tagctatgtc tatagcatta atcttgacaa gacgacgtta
     1441 gggcctggcg atgaatcatt tcccctgcca gatatccttt ggggtgcacc agaactaccg
     1501 ttttatatct atataactga aacgtccctc gccatccttc atattttgat gtagtaacac
     1561 cattcacttt catctacccc ccgttttatt aacatatagt aaaaatcacc tcgccgcatt
     1621 ttattctttt gaagtaaaat catttaccag ttcacttaaa gctctctttt gttcatcatc
     1681 atacagagat tgaatccgga atttaatctc ttctgcaacc agtcttgcat cgggctcata
     1741 aaatattttt gttattcgct gtttctgatg ttcaggctgg tcttccatat ttcgtaaaac
     1801 cagatgaaat aatatatcat aaagtgcgca atgccatgaa taaccatcat tggttaaaat
     1861 aagctcccaa acccgctcct gctcggcaat aagctgcaga acaaaggact caacctgttc
     1921 tgtttgttcg ggggcaacac ggtatagcgg agcaatcaca gcaaaagata gttcatttaa
     1981 ccaggattcc gcttccttct cgctcctgaa cggtggacag ttttgtattc ttcgttcgta
     2041 ttcacgggta atctgctcca gaatatcctc cggcggtgtc agccacttat tcctgagcaa
     2101 tacactggtt gatggatgac agctggctat ttcagcaata atttcacgta atgtctccgg
     2161 gctgaacttc ttcagctgat tgtagtgctc ccgggtaaaa ccatgttcat cgcataaaag
     2221 ttgccatgtc attgatatac tgtttgccat tccttgcttc atcattaata cctgttacct
     2281 gaatatcaac agaccggtgt accttttctg aatacctccc cggacggagt ggcaatcccc
     2341 attcgcctca gcgtggcatc tgcagctttc gccacctcgc tgatatccag cgtttcaggt
     2401 agcggcgggg acgatatctg cgccagacaa taattccggc gcaacaacaa ccactcccgt
     2461 tgtgcttctg tacattcaac atttcccata acaaaaagcc agcggtcaga agctctgcgc
     2521 cagcaaccag cagcctccag tttctgcgc
//
LOCUS       DAZKIP010000269         2545 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZKIP010000269 DAZKIP010000000
VERSION     DAZKIP010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.272"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(201..458)
                     /locus_tag="ACHOUK_004889"
     CDS             complement(201..458)
                     /locus_tag="ACHOUK_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900140.1"
                     /translation="MSDRVIECASRAGRDFSEFMKGEKGMMEALASVDEFGEQLRLNG
                     CVNHHFVSYMMRNSIMQAFMDMAKAERKEERRRKRAEAKAK"
     gene            complement(455..952)
                     /locus_tag="ACHOUK_004890"
     CDS             complement(455..952)
                     /locus_tag="ACHOUK_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFP9900141.1"
                     /translation="MKSLTLFNQPIRIGEDGMICLTDMWKASGKSESESPYHYLRNKQ
                     TKEFLAELEKNHESVVFTERGAHGGTYGGKFVAYDYAAWLNPGFKYAAYKVLDDYFTG
                     ELHHRNSLSAQLNMKCHEFDQKKDMASFCGQGLAAWRYTKPGLIAEINSLANQLQISI
                     PGLPG"
     gene            complement(1155..1592)
                     /locus_tag="ACHOUK_004891"
     CDS             complement(1155..1592)
                     /locus_tag="ACHOUK_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFP9900142.1"
                     /translation="MSRVTAIIYVLVICLIVCLSWAVNHYRDNAIAYKEQRDKATSII
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVSATCAKSTTGASSM
                     GDGESPGLTADAELNYYRLRGGIDKITAQVNYLQEYIRTQCLK"
     gene            complement(1589..2086)
                     /gene="rrrD"
                     /locus_tag="ACHOUK_004892"
     CDS             complement(1589..2086)
                     /gene="rrrD"
                     /locus_tag="ACHOUK_004892"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFP9900143.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMPGKTYTEAECKALLNKDLATVARQINPYIKIDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(2086..2301)
                     /locus_tag="ACHOUK_004893"
     CDS             complement(2086..2301)
                     /locus_tag="ACHOUK_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP9900144.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            complement(2378..>2545)
                     /locus_tag="ACHOUK_004894"
     CDS             complement(2378..>2545)
                     /locus_tag="ACHOUK_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFP9900145.1"
                     /translation="ARLDAEVDAILDELLGVQAEPPTEAGDTTAESGEVQPESPVADA
                     TEPQPESVMML"
BASE COUNT          585 a          681 c          596 g          683 t
ORIGIN      
        1 ttttgattat ccccgcacac tcgcgcagaa ggaattcccc gtcgggctac ggtcatggtt
       61 aatgcggaaa tacagcgacg atacagcgca tgatgtgtca ggcttgaata cctttatccg
      121 ttaaaaggga tatcagttaa gttatcccgt gtagggtata agccattatc aagcccacca
      181 gtagatgggc tttgtaatgg ctacttcgct tttgcttccg ctcgcttacg ccggcgctct
      241 tctttcctct cggcttttgc catgtccatg aatgcctgca tgatcgagtt ccgcatcatg
      301 tagctaacaa agtgatgatt gacacagccg ttgaggcgca gctgctcgcc aaactcatcc
      361 accgaggcca atgcttccat catacccttc tcgcctttca tgaactctga gaagtcgcgc
      421 cccgctctgg aggcgcattc aatgacacga tcactcatcc cggaagcccg gggatcgaaa
      481 tctgcaactg gttagccagg gagttaattt cagcgatcaa accaggtttc gtgtagcgcc
      541 atgccgcgag gccttgtcca cagaagctcg ccatgtcttt tttctgatca aactcatgac
      601 atttcatgtt gagctgcgca cttaagctgt tccgatgatg aagctctccg gtgaagtaat
      661 catcgaggac tttataggct gcatatttaa atccggggtt tagccatgct gcgtaatcat
      721 aagcaacgaa ctttccgcca taagttccac cgtgcgcacc gcgttccgta aaaaccacag
      781 attcgtggtt tttctccagc tcagccaaga actctttggt ctgcttgttt cgcaggtagt
      841 ggtacggaga ttcagattca cttttaccac tggctttcca catatcagtg aggcagatca
      901 tgccatcttc accgatacgg attggttgat tgaagagggt taatgatttc atagcgtgta
      961 cctactcttt gaaatgaacc tttgccgcac aggaaaccag cccaccgagg ctcgccagca
     1021 ctaactggta tcctcaaagg cccattccaa aggggcaggt tcggtgtaaa aaacatgcgt
     1081 tgcggtacgc atttattgca aaaagccccg catcgcgagg ctcattaaat ggactttgtg
     1141 atttgcaaaa aaaattattt caggcattgc gtcctgatgt actcctgcag gtagttaacc
     1201 tgcgcggtta tcttgtcgat tccacctcgg agacggtaat aattgagttc agcatctgct
     1261 gtaagtcctg ggctttctcc atcgcccatg ctgctggctc cggtcgttga ctttgcacag
     1321 gtggcggaga cttgcaggcg cttacgccca gcagaaacat cagcacggag actttcgata
     1381 gtcgcattag catcagcaag ctcctttgtg tatctggcgt caagttctgc tacatcacgt
     1441 tgccgcttct gcatatcagc gatgatggat gtggctttat cgcgctgctc tttgtaggcg
     1501 atggcgttat cacggtaatg attaacagcc catgaaaggc agacgatgag gcagatgacc
     1561 agaacgtaga taatcgcggt tactctgctc attgttgccc ccacaaacag acttcacgct
     1621 caatctcacg gcgagtcatc agccctttcc attgcttacc gccagcgtat gtccagcgcc
     1681 gtagctgatc acatgcgcct ttgatatcgc cctggtttat tttgcgaaga agcgtcgatg
     1741 ttctgaaatt gccagcgccc acgttgtaaa cgaacgagta aagagcgccg cgcgttgttt
     1801 ccggtatatc gattttgatg tacgggttaa tttgtctggc gaccgtggca aggtctttat
     1861 tcaggagggc tttgcattct gcttcggtat acgttttacc gggcatgatg tcttttccgg
     1921 tgtgtccgtg acatacagtc catacaccaa caatatcttt atatggtatg tagctgacac
     1981 cttccagacc atcgttacca cttgggccag taattaacac tgatgctata gcaattgctc
     2041 cgccaccaat agcagcagca acggcttttc gtaatgatgg aggcattatt cacctctcgc
     2101 agccttgcgc ttatcttctt taatcttgaa ataaaggttt gtcaggtacg tcagcaggcc
     2161 aaacagcaga ctccccagca cacctattgc cacccactgg gacggagaga ctttgtccag
     2221 cagctgcagt aaccagtatc ccgtccccac cgctgacgtg gtgtatgaca cacctgttgt
     2281 gattttttcc atctggtaca taccccgtct cccgttatcc ggaagctcac aacatgaaaa
     2341 aggccagcag ctgtttactg atggccctga ctccccgtta cagcatcatg accgattcgg
     2401 gttgaggttc agtcgcatcg gcgaccggtg attcaggctg aacttcaccg ctctctgcgg
     2461 tggtatctcc cgcttcagtc ggtggctctg cctgtacacc aagcagctca tccagaatgg
     2521 catcaacctc tgcatcaaga cgcgc
//
LOCUS       DAZKIP010000270         2534 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZKIP010000270 DAZKIP010000000
VERSION     DAZKIP010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.273"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            373..1350
                     /locus_tag="ACHOUK_004895"
                     /pseudo
     CDS             373..1350
                     /locus_tag="ACHOUK_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041206064.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            1870..>2534
                     /locus_tag="ACHOUK_004896"
     CDS             1870..>2534
                     /locus_tag="ACHOUK_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytotoxin"
                     /protein_id="HFP9900146.1"
                     /translation="MRLPEKVLFPPVTSGLSGQEKQKKPKSITGFQENYQRNIRPIKT
                     ASEARLRFFDKMVSKENSLEDVVSLGEMIQKEIYGHEQRTFSPVHHTGNWKSSLLHNA
                     LLGLANVYNGLRETEYPNTFNRDGIKSTNSFRDNLLTKTRTPRDNFEEGIKHPEHATI
                     PYDNDNESNKLLKAGKIAGNNNELLMEIKKESQSDHQIPLSDKFLKRKKRSPVAEDKV
                     QNS"
BASE COUNT          820 a          532 c          544 g          638 t
ORIGIN      
        1 ctcagccact tgtgtagggg ggctaaaatt aattctattt cattatatga gaacaccaat
       61 aagaataata atatttgatc acaactgaag atgcttttta caatgaagaa ttattgttaa
      121 aaaacgcttt gttgaatcaa ccgaactgtt gctgagttgc aggatcacat cacgtatttt
      181 cctgaaaacg caggctgctt agtagcaaag cagaagttca aaatcaccaa ctaaatagct
      241 gcgccgaata gtagatcaat gaaagggaac tcagcccgga ttgtgcgatc tgatcaatcg
      301 ccaaaaatcc ccccctaacc ggactgagcg atgccgatca tagcaccaat tccccgcgac
      361 gaatgacgcc tgatgcaaaa agcaatctat aaaactcacg ataaaaatta tgcccgcagg
      421 ctgaccgcca tgctgatgct gcaccggagc gctcgcgtca gtgacgttgc cagaacgctc
      481 tgctgcgccc gttcctccgt caggcgctgg attaactggt tcacgctgtc gagtgttgaa
      541 ggtctgaaat cattacccgc aggacgttcc cgccgctggc cgtttgagca tatctgcaca
      601 ttgtttcgtg aactgataaa acactctccc ggatattttg gctatcagcg ttcacgctgg
      661 agtacagaac tgctggcaat aaaaatcaac gagataaccg agtgccagtt acatgccgga
      721 accgttcgcc gctggttgcc attagtcggg cttgtgtggc gcagggctgc gccaaccctg
      781 cgtacccgta acccgcataa agcagcaatt cacaaagcac tggacgaatg tagtgcggag
      841 catccggtct tttatgaaga tgaagtgtat atctatctta atcccaaaac cggtgcggac
      901 tggcaacttc gcagacagca aaaacatgtg gtaacaccgg ggcagaatga aaaatactat
      961 ctggctggtg ctttacatag tggaacgggc aaagtcagct atgtgggtgg caacagcaaa
     1021 agctcagcct tatttatcag tctgttgaaa catctgaaat tgacataccg acgggataaa
     1081 cctatcacgc tcatagtgga caactacatc atccataaaa gtcgggaaac acagcgctgg
     1141 ctgaaagaaa atccgaagtt cagagtgatt tatcagccag tttactcgcc gtgggtgaat
     1201 catgttgagc ggttatagca ggcgcttcac gatacgataa cgcgcaatca tcagtgccgt
     1261 tcaatgtggt aactgcttaa aaatgttcat tattttatgg aaaccgtgag cccattcccc
     1321 ggtggtaaat atgggctgtt aaaagtgtag cggtattcgg cgcagctatt taggatggtc
     1381 aggtcgcaga agttctgtgc cactacagtg cgtgtactaa acaaaatgac gagggcacga
     1441 atgtcataat acgtgcgtat tacctgacag tagctaccaa ctgagggggc ttattccaaa
     1501 cctgatttat tcaacaaagc tggacttatc cttactctga attattttcc agacatatgc
     1561 atcatgtact ggctcgcttg gttcaggaaa tacaggagca aatctacagc tattagattt
     1621 attcctattt gtatgcaaag tcgcattgca taatgcggag ctatgttaca gatacgcctg
     1681 acgtgcgtag taaaattata aagcaacata tatatacaag gagaaaccga gaaaaataaa
     1741 tgagtcgtat gttgggtagc atcgagataa atattttttt tatcacatta gcaccttggt
     1801 taagggtgtt aataataata ttctctcact tattaaaaca taaataattt tttaagcgga
     1861 aatgtaatta tgagactgcc agagaaagtt ctttttcctc ctgtcactag tggcctgtca
     1921 gggcaggaaa aacaaaaaaa accgaagagc attaccggat ttcaggaaaa ttatcaacgc
     1981 aatatcaggc caatcaaaac agcatcagaa gcccgactac gcttctttga taaaatggtt
     2041 tcgaaagaaa actctctgga agatgttgtt tctttaggtg aaatgattca gaaggaaatt
     2101 tatgggcatg aacaaagaac attttcacca gttcatcata caggtaactg gaaatcatca
     2161 ttgttacaca acgcgctcct tggtctggca aatgtttata atggcttacg ggaaacagaa
     2221 taccctaaca ctttcaacag agatggtata aaaagtacta actcttttag agataactta
     2281 ttgacaaaaa caagaactcc cagagataat tttgaggaag gaataaaaca tcctgaacat
     2341 gcaacaatac catatgacaa cgacaatgaa agtaataaat tgctaaaagc aggaaagata
     2401 gctggtaaca ataacgagct gttgatggaa ataaaaaagg aatcccaaag cgaccatcaa
     2461 atccccctgt cagataagtt cctgaaaagg aaaaaacgat ctcctgtagc tgaagataaa
     2521 gttcaaaact cgtt
//
LOCUS       DAZKIP010000271         2432 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZKIP010000271 DAZKIP010000000
VERSION     DAZKIP010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.274"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..2236
                     /locus_tag="ACHOUK_004897"
     rRNA            <1..2236
                     /locus_tag="ACHOUK_004897"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            2331..>2432
                     /gene="rrf"
                     /locus_tag="ACHOUK_004898"
     rRNA            2331..>2432
                     /gene="rrf"
                     /locus_tag="ACHOUK_004898"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          622 a          544 c          766 g          500 t
ORIGIN      
        1 agacccgaaa cccggtgatc tagccatggg caggttgaag gttgggtaac actaactgga
       61 ggaccgaacc gactaatgtt gaaaaattag cggatgactt gtggctgggg gtgaaaggcc
      121 aatcaaaccg ggagatagct ggttctcccc gaaagctatt taggtagcgc ctcgtgaatt
      181 catctccggg ggtagagcac tgtttcggca agggggtcat cccgacttac caacccgatg
      241 caaactgcga ataccggaga atgttatcac gggagacaca cggcgggtgc taacgtccgt
      301 cgtgaagagg gaaacaaccc agaccgccag ctaaggtccc aaagtcatgg ttaagtggga
      361 aacgatgtgg gaaggcccag acagccagga tgttggctta gaagcagcca tcatttaaag
      421 aaagcgtaat agctcactgg tcgagtcggc ctgcgcggaa gatgtaacgg ggctaaacca
      481 tgcaccgaag ctgcggcagc gacactgtgt gttgttgggt aggggagcgt tctgtaagcc
      541 tgtgaaggtg tgctgtgagg catgctggag gtatcagaag tgcgaatgct gacataagta
      601 acgataaagc gggtgaaaag cccgctcgcc ggaagaccaa gggttcctgt ccaacgttaa
      661 tcggggcagg gtgagtcgac ccctaaggcg aggccgaaag gcgtagtcga tgggaaacag
      721 gttaatattc ctgtacttgg tgttactgcg aaggggggac ggagaaggct atgttggccg
      781 ggcgacggtt gtcccggttt aagcgtgtag gctggttttc caggcaaatc cggaaaatca
      841 aggctgaggc gtgatgacga ggcactacgg tgctgaagca acaaatgccc tgcttccagg
      901 aaaagcctct aagcatcagg taacatcaaa tcgtacccca aaccgacaca ggtggtcagg
      961 tagagaatac caaggcgctt gagagaactc gggtgaagga actaggcaaa atggtgccgt
     1021 aacttcggga gaaggcacgc tgatatgtag gtgaagtccc tcgcggatgg agctgaaatc
     1081 agtcgaagat accagctggc tgcaactgtt tattaaaaac acagcactgt gcaaacacga
     1141 aagtggacgt atacggtgtg acgcctgccc ggtgccggaa ggttaattga tggggtcagc
     1201 gcaagcgaag ctcttgatcg aagccccggt aaacggcggc cgtaactata acggtcctaa
     1261 ggtagcgaaa ttccttgtcg ggtaagttcc gacctgcacg aatggcgtaa tgatggccag
     1321 gctgtctcca cccgagactc agtgaaattg aactcgctgt gaagatgcag tgtacccgcg
     1381 gcaagacgga aagaccccgt gaacctttac tatagcttga cactgaacat tgagccttga
     1441 tgtgtaggat aggtgggagg ctttgaagtg tggacgccag tctgcatgga gccgaccttg
     1501 aaataccacc ctttaatgtt tgatgttcta acgtggaccc gtgatccggg ttgcggacag
     1561 tgtctggtgg gtagtttgac tggggcggtc tcctcctaaa gagtaacgga ggagcacgaa
     1621 ggttggctaa tcctggtcgg acatcaggag gttagtgcaa tggcataagc cagcttgact
     1681 gcgagcgtga cggcgcgagc aggtgcgaaa gcaggtcata gtgatccggt ggttctgaat
     1741 ggaagggcca tcgctcaacg gataaaaggt actccgggga taacaggctg ataccgccca
     1801 agagttcata tcgacggcgg tgtttggcac ctcgatgtcg gctcatcaca tcctggggct
     1861 gaagtaggtc ccaagggtat ggctgttcgc catttaaagt ggtacgcgag ctgggtttag
     1921 aacgtcgtga gacagttcgg tccctatctg ccgtgggcgc tggagaactg aggggggctg
     1981 ctcctagtac gagaggaccg gagtggacgc atcactggtg ttcgggttgt catgccaatg
     2041 gcactgcccg gtagctaaat gcggaagaga taagtgctga aagcatctaa gcacgaaact
     2101 tgccccgaga tgagttctcc ctgacccttt aagggtcctg aaggaacgtt gaagacgacg
     2161 acgttgatag gccgggtgtg taagcgcagc gatgcgttga gctaaccggt actaatgaac
     2221 cgtgaggctt aaccttacaa cgccgaaggt gttttggcgg atgagagaag attttcggcc
     2281 tgatacagat taaatcagaa cgcagaagcg gtctgataaa acagaatttg cctggcggcc
     2341 ttagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg
     2401 atggtagtgt ggggtctccc catgcgagag ta
//
LOCUS       DAZKIP010000272         2422 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZKIP010000272 DAZKIP010000000
VERSION     DAZKIP010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.275"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..1210
                     /gene="escC"
                     /locus_tag="ACHOUK_004899"
     CDS             <1..1210
                     /gene="escC"
                     /locus_tag="ACHOUK_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE outer membrane
                     ring protein EscC"
                     /protein_id="HFP9900147.1"
                     /translation="ITPTYLDIDSLLKYLSDTISVNKNSCNVRKITTFNSIEVRGVPE
                     CIKYITSLSESLDKEAQSKAKNKDVVKVFKLNYASATDITYKYRDQNVVVPGVVSILK
                     TMASNGSLPSTGKGAVERSGNLFDNSVTISADPRLNAVVVKDREITMDIYQQLISELD
                     IEQRQIEISVSIIDVDANDLQQLGVNWSGTLNAGQGTIAFNSSTAQANISSSVISNAS
                     NFMIRVNALQQNSKAKILSQPSIITLNNMQAILDKNVTFYTKVSGEKVASLESITSGT
                     LLRVTPRILDDSSNSLTGKRRERVRLLLDIQDGNQSTNQSNAQDASSTLPEVQNSEMT
                     TEATLSAGESLLLGGFIQDKESSSKDGIPLLSDIPVIGSLFSSTVKQKHSVVRLFLIK
                     ATPIKSASSE"
     gene            1224..1679
                     /gene="cesD"
                     /locus_tag="ACHOUK_004900"
     CDS             1224..1679
                     /gene="cesD"
                     /locus_tag="ACHOUK_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone CesD"
                     /protein_id="HFP9900148.1"
                     /translation="MSRKFSSLEDIYDFYQDGGTLASLTNLTQQDLNDLHSYAYTAYQ
                     SGDVITARNLFHLLTYLEHWNYDYTLSLGLCHQRLSNHEDAQLCFARCATLVMQDPRA
                     SYYSGISYLLVGNKKMAKKAFKACLMWCNEKEKYTTYKENIKKLLGNTE"
     gene            complement(2063..>2422)
                     /gene="grlA"
                     /locus_tag="ACHOUK_004901"
     CDS             complement(2063..>2422)
                     /gene="grlA"
                     /locus_tag="ACHOUK_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE transcriptional
                     regulator GrlA"
                     /protein_id="HFP9900149.1"
                     /translation="YDGEPLYILVSLWCKLQEKWISRNDIAEAFGINLRRASFIITYI
                     SRRKEKISFRVRYVSYGNLHYKRLEIFIYDVNLEAVPIESPGTTGPKRKTYRVGNGIV
                     GQSNIWNEMIMRRKKES"
BASE COUNT          802 a          471 c          435 g          714 t
ORIGIN      
        1 aattacacca acatatctgg atattgactc actgctaaaa tatctgagtg atacaatctc
       61 agtaaacaaa aactcttgca atgtgagaaa aattacgaca tttaactcga ttgaagttcg
      121 gggtgttcct gaatgtataa aatatattac atctttatca gaaagtcttg ataaagaggc
      181 acagtctaaa gccaaaaata aagatgttgt taaggtcttt aaactgaact atgcgtcagc
      241 gacagatata acatacaaat accgtgacca aaatgttgtc gtcccaggtg ttgtctccat
      301 tctgaagaca atggcaagta atggttcact tcccagtacc ggtaaaggtg ccgtcgagcg
      361 aagcggtaac ctgtttgata acagtgtaac gatttccgct gatccacgtc ttaatgcagt
      421 tgttgtaaaa gaccgcgaaa taacgatgga tatctatcag caattaattt ctgagcttga
      481 tattgaacaa aggcaaattg aaatttctgt ctcaattatt gatgttgatg caaacgatct
      541 tcagcagctc ggcgtaaact ggtccggtac gttaaatgca ggacaaggta cgattgcctt
      601 taatagctct accgcacagg ctaatatcag ctcttctgtg ataagtaatg ccagtaattt
      661 tatgattagg gtgaatgcgt tacaacagaa ctcgaaagct aaaatactct ctcagccttc
      721 aattataacc ttaaataata tgcaggcgat tcttgataaa aacgtaacgt tttatacaaa
      781 agtctccgga gaaaaggtgg cgtctctgga aagtattaca agcggaacat tgctgagagt
      841 gacaccacgt atacttgatg acagctcgaa tagccttacc ggaaaacgtc gggaacgagt
      901 tcgtttgtta cttgatattc aggatggcaa tcaatcgact aaccaaagca atgctcagga
      961 tgccagcagc accttacctg aagtacaaaa ttcagaaatg acaacagaag cgacattaag
     1021 tgcaggagaa agtttattac ttggcggatt tatccaagat aaagaaagtt caagtaaaga
     1081 tggtataccg ctgttaagcg acattcctgt tattggtagt ttattctcca gtacggtaaa
     1141 acaaaaacat agtgttgttc gactcttttt aattaaagca accccgataa aatctgcatc
     1201 tagcgaataa taaatgaggc tttatgagca ggaaatttag ctctctagag gatatttatg
     1261 atttctacca ggatggtggc acattagcgt cattaacaaa tctgacacaa caagatctca
     1321 atgaccttca ttcttatgcc tatacagcat atcaatctgg tgatgttata accgcaagaa
     1381 atctattcca tttgctcaca tatctggaac actggaatta tgactacacc ttatctctgg
     1441 gcttatgtca tcagcgttta tcaaatcatg aagatgcaca actgtgtttc gcacgctgtg
     1501 caactttagt tatgcaagat cccagggcat cttattattc tggaattagc tacttactcg
     1561 tcggaaataa gaaaatggcc aagaaagcct ttaaggcttg tttaatgtgg tgtaatgaaa
     1621 aagaaaaata cactacatat aaagaaaata ttaaaaaatt gttaggtaat acagagtaaa
     1681 aactaccatc agctatacca atccccaggc tgactgggga ttttctatcg gaaataataa
     1741 agctgctata gtgaagtgct cccaaagttg agcaccgacc actggtagat ttatctacag
     1801 gtgtggggtt attccattaa ccaggagttt atgccctgaa ctatcttctg ctgtatttaa
     1861 tatcatttcc gtttatgaaa caatcagtat atcttaactt agcattagaa atctgatctt
     1921 tccaatcatc tatagctcag agtatatttg tcaggaaatg accttcaaac tttagccgag
     1981 aaaaaggctt accctggaaa acaaaaccct taaatatagc tttattttta ttcttctata
     2041 aaatatactc aaaaaattac gtctaactct cctttttccg cctcatgatc atttcgttcc
     2101 agatattaga ctgtcccaca ataccattac caactcggta ggtttttctt tttggtccgg
     2161 ttgttccagg actttctatc ggaaccgcct caaggttaac atcataaatg aaaatctcaa
     2221 ggcgcttata atgcaaatta ccataactaa catatctgac acgaaatgaa attttttctt
     2281 ttcttctcga tatataagtt ataataaatg atgctctcct caggtttata ccgaatgctt
     2341 cggcaatatc attgcgagaa atccatttct cctgcaattt acaccaaaga gaaaccaaga
     2401 tatacagagg ttcaccatcg ta
//
LOCUS       DAZKIP010000273         2405 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZKIP010000273 DAZKIP010000000
VERSION     DAZKIP010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.276"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..1833
                     /locus_tag="ACHOUK_004902"
     CDS             <1..1833
                     /locus_tag="ACHOUK_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFP9900150.1"
                     /translation="TLPDGVETYGVWGLSLPSLRRRLFRCVSVRENTDGTFAITAVQH
                     VPEKEAIVDNGARFEPQSGTLNSVIPPAVQHLTVEVSAADGQYLAQVKWDTPRVVKGV
                     RFSLRLTSGSGEGSRLVTTAITADTEHRSSGLPPGEYTLTVRAINSYGQQGEPATTTF
                     RINAPAVPATIELTPGYFQITAVPRLAVYDPTVQFEFWFSETKIADTSQVETSARYLG
                     TGSQWSVSGPHIKPGKDFWFYVRSVNLVGKSAFVEASGRASNDAEGYLGLFREKIGKL
                     HLAQGLWELIDNSQLADEMAEMKTSITETRNEITQTVSKTLEDQSAIIQQIQRVQKDT
                     NDDLAALYMLKVQKTKNGIPYVAGIGAGIEDTDGQPLSNILLLADRIAMINPEDGNTT
                     PLFVAQGNQLFMNDVFLKRLFAVSITSSANPPTFSLTPEGRLTARNADISGNVNANSG
                     TLNNVTINENCRVLGKLSANQIEGDLVKTVGKAFPRDSRAPERWPSGTITVRVYDDQP
                     FDRQIVIPAVAFSGAKHEREHTDIYSSCRLIVRKNGAEIYNRTALDNTLIYSGVIDMP
                     AGHGHMTLEFSVSAWLVNNWYPTASISDLLVVVMKKATAGISIS"
     gene            1901..>2405
                     /locus_tag="ACHOUK_004903"
     CDS             1901..>2405
                     /locus_tag="ACHOUK_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP9900151.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHVSTNVPG
                     SDELNGINVKYRYEFTDTLGMVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNP"
BASE COUNT          566 a          604 c          720 g          515 t
ORIGIN      
        1 accctgcctg atggtgtgga gacatacggt gtatggggac tatccctgcc gtcactgcgc
       61 cgtcgcctgt tccgctgtgt ctccgtccgg gaaaacacgg acggcacctt tgccatcacg
      121 gcggtgcagc acgtaccgga aaaagaagcc atcgtggata acggtgcccg ctttgagccg
      181 cagtcaggca ccctgaacag cgtcatccct ccggcagtgc agcacctgac ggtggaggtg
      241 agcgcagctg acggccagta tctggcgcag gtgaaatggg acacgccgcg ggtggtgaag
      301 ggcgtgcgct tcagtctgcg cctgaccagc ggaagcggag aaggcagccg tctggtgacc
      361 accgccatca ctgcggatac agagcatcgt tccagtggtc tgccgccggg ggaatacacc
      421 ctgacggtca gggcgattaa cagttatggc cagcaggggg aaccggccac caccacgttc
      481 aggattaatg cacctgcggt acccgccacg attgagctga caccgggcta ttttcagata
      541 acagcggtcc cgcgtcttgc ggtgtatgac ccgacggtac agtttgagtt ctggttttcg
      601 gagacaaaaa tcgcagatac atctcaggtg gaaacctctg cccgttatct ggggaccggc
      661 agtcagtgga gtgtatccgg cccgcacatt aagcccggga aggatttctg gttttacgtg
      721 cgcagcgtca acctggtggg gaaatctgct tttgtggaag ccagtggccg ggccagcaat
      781 gatgcagaag ggtatctggg gctgtttcgg gaaaaaatag gaaaactgca tctggctcag
      841 gggctgtggg agctgataga caacagccag cttgcggatg agatggcgga gatgaagacc
      901 tccatcaccg aaacccgcaa tgaaatcaca cagacggtca gtaaaacgct ggaggaccag
      961 agcgccatca tacagcagat acagcgcgtg cagaaggaca caaatgatga ccttgctgca
     1021 ctttacatgc tgaaggtaca gaaaacaaaa aatggcatac cctatgttgc cggtattgga
     1081 gcggggattg aggatactga tggccagccc ctgagcaaca tactgctgct ggctgaccgt
     1141 attgcgatga ttaacccgga ggacggcaac accacgccgt tatttgtggc gcaggggaat
     1201 cagttgttca tgaacgatgt gttcctgaaa cgactgtttg cggtgagtat cacgtcatcc
     1261 gccaatcccc cgacgttttc cctgacgccg gagggcaggc tgaccgcaag aaatgctgat
     1321 atcagcggta acgtgaatgc gaattccggg acgctcaaca acgtcacgat taacgagaac
     1381 tgtcgggttc tgggaaaact gtccgcgaac cagattgaag gcgatctcgt taaaacagtg
     1441 ggcaaagctt tcccccggga ttcccgtgca ccggagcggt ggccatcagg gaccattacc
     1501 gtcagggttt atgacgatca gccgtttgac cggcagattg ttattccggc ggtggcattc
     1561 agcggcgcta aacatgagag agagcatact gatatttact cctcatgccg tctgatagtg
     1621 cggaaaaacg gtgctgaaat ttataaccgt accgcgctgg ataatacgct gatttacagt
     1681 ggcgttattg atatgcctgc cggtcacggt cacatgacgc tggagttttc ggtgtcagca
     1741 tggctggtga ataactggta tcccacagca agtatcagcg atttgctggt tgtggtgatg
     1801 aagaaagcca ccgcaggcat cagtatcagc tgaattttat aacccatata cgggcgccag
     1861 aaatggcgcc ttttttattg cagaaaagcg agaggtaatt atgcgtaaac tttatgccgc
     1921 cattttgtcc gcagccattt gtctggccgt atccggtgcg cctgcatggg cgtctgaaca
     1981 tcagtcaacg ctgagcgcgg ggtatcttca tgtctcgacg aacgttcctg gcagcgatga
     2041 actgaacggg attaacgtga aataccgtta tgagtttacg gacacactgg ggatggtgac
     2101 gtcgttcagc tatgcaggag acaagaatcg ccagctgacc cgttacagcg atacccgctg
     2161 gcatgaagat tccgttcgta accgctggtt cagcgtaatg gcggggccgt ctgtgcgcgt
     2221 gaatgaatgg ttcagcgcgt atgcgatggc gggtgtggct tacagccgtg tgtcgacttt
     2281 ctccggggat tatctccgcg taactgacaa caaggggaaa acgcatgatg tgctgaccgg
     2341 aagtgatgac ggtcgccaca gcaacacgtc tctggcgtgg ggagctggcg tgcagtttaa
     2401 cccga
//
LOCUS       DAZKIP010000274         2330 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZKIP010000274 DAZKIP010000000
VERSION     DAZKIP010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.278"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..99
                     /locus_tag="ACHOUK_004904"
     CDS             <1..99
                     /locus_tag="ACHOUK_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900152.1"
                     /translation="LKEFNICPEKVAEYLSEFSGKHFLDVYNYYSN"
     gene            281..466
                     /locus_tag="ACHOUK_004905"
     CDS             281..466
                     /locus_tag="ACHOUK_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFP9900153.1"
                     /translation="MELLLSLRIRSLIFLQKLIGRNNLTQRTRIKRLARRIIYFSRTV
                     VRNKKVIGTFIKKHIFY"
     gene            707..1696
                     /gene="nleB"
                     /locus_tag="ACHOUK_004906"
     CDS             707..1696
                     /gene="nleB"
                     /locus_tag="ACHOUK_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB"
                     /protein_id="HFP9900154.1"
                     /translation="MLSSLNVLQSSFRGKTALSNSTLLQKVSFAGKEYPLEPIDEKTP
                     ILFQWFEARPERYEKGEVPILNTKEHPYLSNIINAAKIENERIIGVLVDGNFTYEQKK
                     EFLSLENEYQNIKIIYRADVDFSMYDKKLSDIYLENIHKQESYPASERDNYLLGLLRE
                     ELKNIPEGKDSLIESYAEKREHTWFDFFRNLAMLKAGSLFTETGKTGCHNISPCSGCI
                     YLDADMIITDKLGVLYAPDGIAVHVDCNDEIKSLENGAIVVNRSNHPALLAGLDIMKS
                     KVDAHPYYDGLGKGIKRHFNYSSLHDYNAFCDFIEFKHENIIPNTSMYTCSSW"
     gene            1745..>2330
                     /gene="nleE"
                     /locus_tag="ACHOUK_004907"
     CDS             1745..>2330
                     /gene="nleE"
                     /locus_tag="ACHOUK_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase NleE"
                     /protein_id="HFP9900155.1"
                     /translation="MINPVTNTQGVSPINTKYAEHVVKNIYPEIKHDYFNESPNIYDK
                     KYISGITRGVAELKQEEFVNEKARRFSYMKTMYSVCPEAFEPISRNEASTPEGSWLTV
                     ISGKRPMGQFSVDSLYNPDLHALCELPDICCKIFPKENNDFLYIVVVYRNDSPLGEQR
                     ANRFIELYNIKRDIMQELNYELPELKAVKSEMIIA"
BASE COUNT          809 a          369 c          446 g          706 t
ORIGIN      
        1 ttaaaagaat tcaatatatg cccagaaaag gtagcagaat acctaagcga gttcagcgga
       61 aaacatttcc tcgatgtata taattattat tccaattgaa aaaaatataa agtatctatc
      121 acggcactct cagttgagta tcacaggcac atgtatttta gccccctgag tctacagaca
      181 accaatatta cttaaataag tgataatctt aatgctgttt tttagaacag atgattgata
      241 tttttgggcc ggagaaacgc agacaggctt tgttgaataa atcgagcttt tgctgagttt
      301 aaggatcaga tcactcattt tcctgcaaaa gctcattggg cgaaacaatc tgactcagag
      361 aacccggatt aaacgtctgg cccgcagaat catctatttc tcacgcactg tagtacgtaa
      421 taaaaaagtc atcggtactt ttatcaaaaa acatatattc tattaattat tgccatcacc
      481 tttttcttat tgacaacatt actctgctga ttgttaaaat ctaacctgta ttcagggtta
      541 cgattctata caatagaaca gggaggggaa aggatgtcat cgttaatgag actttttgtt
      601 aatgtcaaca atgctatgaa tttattttag aaaaacaatt aagctgacgt agtgttccgt
      661 ctaaatgacg gggcgatatt aacatgatta gaacaagagg aattttatgt tatcttcatt
      721 aaatgtcctt caatccagct tcagaggaaa gacagcttta tcaaatagta cacttctcca
      781 gaaagtttct tttgctggaa aagaatatcc tctggaacct attgatgaaa aaacccctat
      841 tctttttcag tggtttgaag caaggccaga gcgatacgaa aaaggagaag taccaatatt
      901 gaataccaaa gaacatccgt atttgagcaa tattataaat gctgcaaaaa tagaaaatga
      961 gcgtataatc ggtgtgctgg tagatggaaa ttttacttat gaacaaaaaa aggaatttct
     1021 cagtcttgaa aatgaatatc aaaatataaa aataatctac cgagcagatg tggatttcag
     1081 catgtatgat aaaaaactat ctgatattta ccttgaaaat atccataaac aagaatcata
     1141 ccctgccagt gagagggata attatctgtt aggcttatta agagaagagt taaaaaatat
     1201 cccagaaggt aaggactctt tgattgagtc atatgcagaa aaaagagaac atacttggtt
     1261 tgattttttc aggaatttgg ccatgttgaa ggctggaagt ttgtttacag agacgggaaa
     1321 aactggatgc cataacatat cgccctgtag cggatgtata tatcttgatg ccgacatgat
     1381 tattaccgat aaattaggag tcctgtatgc tcctgatggt atcgctgtgc atgtagattg
     1441 taatgatgag ataaaaagtc ttgaaaatgg tgcgatagtt gtcaatcgta gtaatcatcc
     1501 agcattactt gcaggcctcg atattatgaa gagtaaagtt gacgctcatc catattatga
     1561 tggtctagga aagggtatca agcggcattt taactattca tcgttacacg attataatgc
     1621 tttttgtgat tttattgaat ttaagcatga aaatattata ccgaatacca gtatgtatac
     1681 ctgcagttca tggtaagtct tccggttgtg gttgaaaaat atcgttcttt aggtatagat
     1741 tcagatgatt aatcctgtta ctaatactca gggcgtgtcc cctataaata ctaaatatgc
     1801 tgaacatgtg gtgaaaaata tttacccgga aattaaacat gattacttta atgaatcacc
     1861 caatatatat gataagaagt atatatccgg tataaccaga ggagtagctg aactaaaaca
     1921 ggaagaattt gttaacgaga aagccagacg gttttcttat atgaagacta tgtattctgt
     1981 atgtccagaa gcgtttgaac ctatttccag aaatgaagcc agtacaccgg aaggaagctg
     2041 gctaacagtt atatccggaa aacgcccaat ggggcagttt tctgtagata gtttatacaa
     2101 tcctgattta catgcattat gtgagcttcc ggacatttgt tgtaagatct tccctaaaga
     2161 aaataatgat tttttataca tagttgttgt gtacagaaat gacagccctc taggagaaca
     2221 acgggcaaat agatttatag aattatataa tataaaaaga gatatcatgc aggaattaaa
     2281 ttatgagtta ccagagttaa aggcagtaaa atctgaaatg attatcgcac
//
LOCUS       DAZKIP010000275         2253 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZKIP010000275 DAZKIP010000000
VERSION     DAZKIP010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.279"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            95..>2253
                     /locus_tag="ACHOUK_004908"
     CDS             95..>2253
                     /locus_tag="ACHOUK_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFP9900156.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFLVCEDKPGIPRPALDSWDAEYMQENRWDEGAASFVPVETESDPM
                     NVAFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCKPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RVHRQHTENSAEKIPPVTAVIRREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KNEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEDEGTNQSATDTLSSPVPETDAVETAIPDNEKTECKVEVEPSVEREGPFYF
                     LFTDKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTGLPQNANTAQNSEQ
                     PEPVKVTADEVKKIMQAANISQPDANQLLAASRGEFVAGISDPNDPKWVKGIETRDSV
                     NQNQQETEQNDQKAEQNSPNTQQNEPETKQPEPVAQQEPEKVCTACGQTGGGNCPDCG
                     AVMGDATYQETFDEEYQVEVQEDDPEEMEGAEHPHKENTDGNQHHDSDNETGETADHS
                     IKVNGHQEITSTSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTID
                     LETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDAL"
BASE COUNT          700 a          543 c          564 g          446 t
ORIGIN      
        1 aaaacagttc cgtaataaac atccgatttc attcttatat gccagcaatg gcagggattt
       61 gttcaccctt aaatctgtaa tgaggtaaaa caaaatgagt aaagtcttta tttgcgccgc
      121 cattccggac gaacaggcaa taaaggaaga aggtgcagtc gctgtagcca ctgccattga
      181 agccggtgat gaacgtcgcg cccgcgcaaa atttcactgg caattcctgg agcattatcc
      241 ggctgctcag gactgcgctt ataaatttct tgtttgcgag gataaacccg gtataccccg
      301 ccctgccctc gattcctggg atgctgaata tatgcaggaa aaccgctggg atgagggggc
      361 tgcttccttt gtcccggttg agactgaatc cgatccgatg aacgtcgctt ttgacaagct
      421 ggcccctgaa gtacagaacg ctgtcatggt taagttcgac acatgtgaaa acatcaccgt
      481 tgatatggtg attagcgcgc aggaattgtt gcaggaagac atggcaacat tcgacggaca
      541 tatcgttgaa gcgttgatga aaatgccaga agttaacgcc atgtatccgg agcttaagct
      601 gcatgccatc gggtgggtta agcataaatg taagcctggt gccaaatggc ccgaaattca
      661 ggcagaaatg cgcatctgga aaaaacgtcg cgaaggtgaa cgcaaggaaa ccggaaaata
      721 cacgtctgtt gttgatctcg cccgcgccag agtccaccga cagcacactg aaaactcagc
      781 agaaaaaatc ccccctgtca ctgcagtcat tcgtcgcgaa tataagcaga catggaaaac
      841 actggatgac gaactggcct acgctctctg gcctggtgat gtggatgccg gaaacattga
      901 cggcagcatc catcgctggg caaaaaatga agttatcgac aacgaccgcg aagactggaa
      961 gcgtatctcg gcatcaatgc gcaaacagcc tgatgccctt cgctacgacc gccagactat
     1021 ttttggcctt gtccgtgaac gtccgatcga cattcacaaa gaccctgtgg cactgaacaa
     1081 atacattact gaatacctga ctacaaaggg cgtgtttgaa gatgaaggaa caaatcagag
     1141 cgcaactgac actctctcgt cgccagtacc agaaactgat gcagtggaaa cggcaattcc
     1201 ggacaacgaa aaaaccgaat gcaaagtgga agtcgaacca tctgtagagc gtgaggggcc
     1261 gttctacttc ctcttcaccg acaaggatgg cgaaaaatac ggtcgcgcaa acaaactttc
     1321 tggtctggat aaggcgctgg ctgccggggc tactgaaatc acaaaagaag aatattttgc
     1381 ccgaaaaaat ggcacataca caggcttacc gcaaaatgca aataccgcac aaaattctga
     1441 acaaccagaa ccggtaaaag ttaccgctga cgaagtaaag aaaattatgc aggcagccaa
     1501 tatcagccag cctgacgcca atcagttgct cgccgcatca cgtggtgaat ttgttgcagg
     1561 gattagcgac ccgaatgatc cgaaatgggt gaaggggatt gaaacccgcg attctgtgaa
     1621 ccagaaccag caagaaacgg aacagaacga ccagaaagcg gaacaaaaca gcccaaatac
     1681 gcaacaaaac gagccagaaa cgaaacaacc tgagccagta gcgcaacagg aaccggaaaa
     1741 agtctgcacc gcctgcggtc agaccggcgg cggcaactgc cctgattgtg gcgcggtgat
     1801 gggcgacgca acataccagg aaacattcga tgaagagtat caggttgaag ttcaggaaga
     1861 tgatccggag gaaatggaag gcgctgaaca tccacacaag gagaacactg acggcaatca
     1921 gcatcacgat agcgataatg aaactggcga gacggcagat cactcaatta aggtgaacgg
     1981 tcatcaagaa atcacatcca ccagcaggac gtgtgaccat ctaatgatcg accttgaaac
     2041 catgggaaaa aatcctgatg ccccgatcat ctcaataggt gcaatatttt tcgatccgca
     2101 aaccggagat atgggaccgg aatttagtaa gactatcgat ctggaaactg ctggcggggt
     2161 cattgatcgg gacaccatta aatggtggct taagcaatca cgcgaagcgc aatctgccat
     2221 tatgaccgat gaaatcccgt tagatgatgc act
//
LOCUS       DAZKIP010000276         2247 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZKIP010000276 DAZKIP010000000
VERSION     DAZKIP010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.280"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>2247
                     /locus_tag="ACHOUK_004909"
     CDS             <1..>2247
                     /locus_tag="ACHOUK_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3491 domain-containing protein"
                     /protein_id="HFP9900157.1"
                     /translation="KYIKLPLVQTGSLLRDIYLTTPEKLSTSGGSLNIMGHLVPVSFI
                     YDIGGVINGNRISESTDVKNKIRSLKINGDILQHYINTHYLSEEQTQKIKDIVDFLGI
                     QDNTIKVKLESDIKPISEIQQPLHSILSRQKEHVKNLLSGLLDEFSNKLRKQGLSLKT
                     NVLSVNNFKESKINSDTVEVTVTDLQGRLYRVDIDTRVIGLTFKEGINSLSEALEHMN
                     IDAIMSVIGLVQYARMIKMNDNISAIDHAGAVSDIKNIVDKFLGGILTLTNNRVYNPG
                     GVSGASLEGFISSGLEVCASRMGGTAGRYLSNVAKVIKLPLLDIGINIWSLYDSSLNH
                     AKATTQIEYISTAIDVSFSSINTALSIGAIAYPPLAIAIVPITIFSHEVKNYAVYVNQ
                     INERHKLWLEAEKYLDNGSAKVLSINKATGIIDLSNNQVLGNIYLDMRENPPILHGEK
                     SYNSGKNIGSHPDMTDREIMESRAYNFACTKLSDAGEPDIFGWGDKEICNSMELSESQ
                     LANGYSNRQWPSQIPVIPEGIYNTVYLGYGETLRANTEVTLSSTGYFYEIARAYTDDE
                     LSEPLLTVCNQHSHVIGGKEPLTIIIPAIEHGMLGSNLHMIERFKNYNFSISGGKGGI
                     KLMVGGIGDYNIECTPGVRNIISFEQLSRDFNLDLDLSDGRKQNFNFHHPSGFYSGKV
                     MSITQKGINAVVGTKLGYDKIRGNNLDNTFSLGSGGGVIYSGGGSNTYFVPATLQDNL
                     HIYISEKS"
BASE COUNT          776 a          330 c          448 g          693 t
ORIGIN      
        1 gaagtatatt aagctccccc ttgttcagac tggttctttg ttaagagaca tatatttaac
       61 aaccccagaa aaattatcca caagcggggg ttcattaaat attatggggc atctcgtgcc
      121 tgtcagtttt atttatgata tcggaggggt tattaatggt aacagaataa gtgaaagtac
      181 agatgtgaaa aataaaatta ggagcttaaa aataaatggg gatattttac aacattatat
      241 aaatacgcat tatttgtcag aggagcaaac tcagaagata aaagatattg tggatttttt
      301 aggtattcag gataacacaa taaaagttaa gcttgaaagt gatattaaac ctataagtga
      361 aatccaacag ccccttcatt ccattctttc acggcagaaa gaacacgtaa agaatttact
      421 atctggattg cttgatgagt tttcaaataa gttaagaaag caaggtcttt cacttaaaac
      481 aaatgttctt tcagttaata acttcaaaga atcaaagata aatagtgata cagtggaggt
      541 tacagtaaca gatctgcaag gaagacttta tcgtgttgat atagatacaa gagttattgg
      601 cttaactttt aaagagggga taaatagttt atccgaagca ctcgaacaca tgaatatcga
      661 tgcgattatg tctgttattg gcttggttca atatgcacga atgataaaaa tgaatgacaa
      721 tattagtgca atagatcatg caggagcagt atcagatata aaaaatattg tagacaaatt
      781 tttaggcgga attttgactc ttactaataa tcgcgtatat aatcctggcg gtgtatctgg
      841 tgcctcgctt gaaggattta tatcttctgg gttggaggtg tgtgcttcaa gaatgggtgg
      901 caccgcaggg cgttatctta gcaatgtggc aaaagtaatt aaattacctt tgctagatat
      961 aggcataaat atttggtcat tatatgattc ttcattaaat catgctaaag ccaccacaca
     1021 aattgaatat atatcaacag caatagatgt atctttttca tctataaata cagctttgtc
     1081 aataggcgca atcgcatatc ctcctttggc aatagctata gttccgataa ctattttttc
     1141 tcatgaagta aaaaactatg ctgtttatgt gaatcaaatt aatgagcgcc ataagttatg
     1201 gctcgaagct gaaaagtatc tggacaatgg aagtgcaaag gttctaagca ttaacaaagc
     1261 cacaggcata atcgatttat caaataatca ggtgttaggt aatatttatt tagatatgag
     1321 agaaaatcct ccaatattac acggtgagaa atcatataat agcggaaaga atattggtag
     1381 tcatcctgat atgactgaca gagagattat ggagtctaga gcatacaatt ttgcatgcac
     1441 aaaactctca gatgcaggtg aacccgatat tttcggttgg ggagataagg aaatatgtaa
     1501 ttcaatggaa ttatcagaat cacaattagc taatgggtat tctaaccgcc aatggccttc
     1561 tcagattcca gttattccag aaggaattta taatacagta tatcttggat atggagaaac
     1621 attgcgagct aatactgaag ttacattgag tagcactggt tacttttacg agattgcaag
     1681 ggcatatact gatgatgaac tatctgagcc attattaaca gtatgtaacc aacatagtca
     1741 tgttattggt ggaaaagaac cattaacaat tattattccc gctattgaac atggtatgct
     1801 tggctctaat ctacatatga ttgaacgttt taaaaactat aatttctcga tatcaggagg
     1861 gaagggaggg ataaaattaa tggttggtgg tattggtgat tacaacatag aatgtacccc
     1921 cggtgttaga aatattatat cgtttgaaca attatcacgt gacttcaatc tggatttgga
     1981 tttatcagat gggaggaaac aaaattttaa ttttcatcat ccctcaggtt tttattcagg
     2041 aaaagttatg agcataacgc aaaagggtat aaatgcggtt gttggtacta aactgggata
     2101 tgataagatt cgtggtaata atcttgataa taccttttct cttgggagtg gaggaggtgt
     2161 tatatattct ggaggaggga gcaataccta tttcgttccc gccacactac aggataattt
     2221 acacatatat atatcagaaa aatcaaa
//
LOCUS       DAZKIP010000277         2175 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZKIP010000277 DAZKIP010000000
VERSION     DAZKIP010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.281"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..1035
                     /gene="rfaL"
                     /locus_tag="ACHOUK_004910"
     CDS             <1..1035
                     /gene="rfaL"
                     /locus_tag="ACHOUK_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HFP9900158.1"
                     /translation="KHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPIILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVAIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(1071..>2175)
                     /locus_tag="ACHOUK_004911"
     CDS             complement(1071..>2175)
                     /locus_tag="ACHOUK_004911"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HFP9900159.1"
                     /translation="SIPPRGAAAVETWMYQVAQRTNFPNRIVCIKNPGYSNYTFVNDN
                     CSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESVIVVHNSMKLYTQI
                     RKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLANADIEIVPNGIDLE
                     TYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKLAAAHKNLKLVVIG
                     DYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPLADLVVIPSQFQEP
                     FCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSISSDILKTLANPEL
                     TAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
BASE COUNT          663 a          406 c          382 g          724 t
ORIGIN      
        1 aaacactatc tctcactatt taaaacattt ctttttggta gtgttgccat attaactatt
       61 gctgcattgc tgtcacttct tcaatcccct gatgcaggtg ctagcatgaa ggaaattttc
      121 aaagctatta ttgagaatac tttactatgc acaatagcaa taccgatcat attgagagac
      181 gagaaaagag aagatgtcga aaaaatcgtt ttcttctcat ttattagtgc gttgggctta
      241 cgctgttttt ctgaattgat tacctattat aaggactatc aacaagggat aatgccattc
      301 gcagattata gacaccgtag catttctgac tcgatggtct ttttattccc tgcattgtta
      361 aatctctggc ttatcaaatc agcaaaatac cgcatttctt ttgtggttct aagcgttatt
      421 tttatttttc tgatattagg aactttatcc agaggggcct ggctttccgt gttagtcatt
      481 ggattaatat ggattctgat gtttaaacaa tggaagttac tattagtagg agtaatggtt
      541 gccatcattg cattgtcggt tattttcaca cataaggaga tgaccgcaaa gctaacgtat
      601 aaacttcaac aaactaatag ttcttatcgc tatgcaaatg gtactcaagg cagcgcactc
      661 gatctaatat tagaaaatcc tgttattggt tatggttacg gtaacgttgc atataaagat
      721 gtctataata aacgtgtcat tgattatcca gaatggacct ttagacaatc aatagggcca
      781 cataattttg cgctattcat ctggtttggc actggtttat tagggctggt aagtcttatg
      841 atgctatact gtgcaatatt gaaagagtgt ataaaaaatg gcgtcaagaa taaatatcgc
      901 tcaccatata atgcatatta tataatctta ctatctttta taggttattt tgttatccgt
      961 ggaaacgtag aacaaattga accaaattta ttaggcgttt acgccggctt attattagcg
     1021 atgaaaaaca agtaaaaaat aaaaaaggct gcataatgcg gcctttttat ttattcaaac
     1081 caattatgaa taacctcttc gaatctttga gttacgccat cccaactgta gtgatcaaac
     1141 acaaagtcct gaccttgctt tgcaactgct gttaattcag gattcgctaa cgtttttaaa
     1201 atatcgctgc ttatggagtc tgcagtcata ggttctttta aatggaatcc cgtagtattt
     1261 tctttgacaa attccgtcat ccccccacgc gtactcacta aaacaggctt accagcaccc
     1321 atagcttcaa ttgccaccat gcaaaatggt tcctggaact gggaaggaat gacgactaaa
     1381 tctgccagtg ggtaataaca atgcatcttt tcaggcggta tgcttcctaa cataatgcaa
     1441 cgatccttca aacgttttgc aatttccctg acattgcgtt gatatgcacc tttgtcactt
     1501 ttactcattt ctgtataatc accaatcaca actaatttta aatttttgtg agcagcggcc
     1561 aatttttcga atgattgcat caacagcaat atgcctttgt cagggacgat acgcccagca
     1621 taaaaaataa ttttctcttc aggggagata ttaagttcgc ttctagtgat cggctgaaaa
     1681 ttagattgat aggtttctaa atcaattcca tttggtacaa tctcaatatc tgcatttgct
     1741 aaataagatt ggtaatactt tttcaagtat aaactaggca ctatcatttt aacattttgc
     1801 tcaagcaatt ttggttcaaa tgcattatgc atatgtataa ccactcttgc ctgaggagct
     1861 cgcttacgaa tttgagtgta tagcttcatg ctattatgaa ctacaataac actttctttc
     1921 gttataggaa aatcatgtgc aatattcaat attctctgtg aatatggaag aggatcaagt
     1981 cttgtccatt tttgaaataa ccgcttatat atgcgactga atccaaccct atgaatacta
     2041 caattatcat taacaaaggt gtaatttgag tatcctggat ttttgataca aacaatacga
     2101 tttggaaaat tggtacgctg agcaacctga tacatccacg tttcaacagc agccgctccg
     2161 cgaggaggaa ttgaa
//
LOCUS       DAZKIP010000278         2175 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZKIP010000278 DAZKIP010000000
VERSION     DAZKIP010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.282"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..320)
                     /locus_tag="ACHOUK_004912"
     CDS             complement(<1..320)
                     /locus_tag="ACHOUK_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HFP9900160.1"
                     /translation="MANKTEKPTQKKLQDASKKGQILKSRDLTVSVIMLVGTLYLGYV
                     FDVHHIMSILEYILDHNAKPDIWDYFKAMGIGWLKTIIPFLLVCMFTTILVSWFQSKM
                     QLAT"
     gene            complement(329..1096)
                     /gene="sctT"
                     /locus_tag="ACHOUK_004913"
                     /pseudo
     CDS             complement(329..1096)
                     /gene="sctT"
                     /locus_tag="ACHOUK_004913"
                     /inference="COORDINATES: protein motif:HMM:NF013478.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
     gene            complement(1098..1358)
                     /locus_tag="ACHOUK_004914"
     CDS             complement(1098..1358)
                     /locus_tag="ACHOUK_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HFP9900161.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(1369..2034)
                     /locus_tag="ACHOUK_004915"
     CDS             complement(1369..2034)
                     /locus_tag="ACHOUK_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HFP9900162.1"
                     /translation="MSNSISLIAILSLFTLLPFIIASGTCFIKFSIVFVIVRNALGLQ
                     QVPSNMTLNGVALLLSMFVMMPVGKEIYYNSQNENLSFNNVASVVNFVETGMSGYKSY
                     LIKYSEPELVSFFEKIQKVNSSEDNEEIIDDDNISIFSLLPAYALSEIKSAFIIGFYI
                     YLPFVVVDLVISSVLLTLGMMMMSPVTISTPIKLILFVAMDGWTMLSKGLILQYFDLS
                     INP"
     gene            complement(2024..>2175)
                     /locus_tag="ACHOUK_004916"
     CDS             complement(2024..>2175)
                     /locus_tag="ACHOUK_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFP9900163.1"
                     /translation="RIISLLPESEKNIEIRVNGALTGYGELVEVDDKLGVEIHSWLSG
                     HNNVK"
BASE COUNT          754 a          454 c          348 g          619 t
ORIGIN      
        1 tcagttgcta attgcatttt actttgaaac caggagacaa gtattgttgt gaacatgcaa
       61 accagcaaaa aaggaatgat cgttttcaac caaccaatcc ccatagcttt aaaatagtcc
      121 caaatatccg gtttagcgtt atgatcaagg atatattcaa gaatcgacat aatgtgatgc
      181 acatcaaaaa catatccgag atataaagta cccacaagca ttattacaga aacggttaag
      241 tctctacttt ttagaatttg ccctttttta gaagcatcct gcagtttctt ttgtgttggc
      301 ttttcagttt tatttgccat gaaacactct atttctcaat gtatagttgc aattgttctg
      361 ggaaaaagga taacggcatc acacgttcag ccagtattgg gccaaaataa ataattaata
      421 ttaaaaatgc cagcgcactt ttgacagtca gtgaaatggc aaaagcattt agttgtgaag
      481 catagcgtgc cagcaatccc aggacagcct caccacccag cataacggca attacggggc
      541 ttgcgtaaac gatagtatga gtcattatat tattaatata tgaaaataaa ggctctaatt
      601 taggaaaatt aaaacttcct gaaggccata aattataact ttgctaaaga gtttccagaa
      661 taaagttcaa tccaccgtta gtaagataaa ccgctgcaga gaaaaggtta aataatcttg
      721 ctaattccga ggtatcgaca cctgtagccg gatccaaagt actacttaac gttgcaccgc
      781 gttggttatc gataaagcta cctatagcaa ggaaaatcca aaatggagat gctaataagc
      841 aagcaatgaa cagccctatg ataatttctc ttgcagctaa caaagcgata tcgatttcat
      901 ttaaggtcga taattcatag tggtaatgct gaactaatgc acatgatacc actattatta
      961 ttggatgacg gactactgtt ggtatattcc cactggcgaa aaatggtaac aaatagaaag
     1021 ttggtgccaa tcggcaaaaa ccgagcgctg tagcagccag tagactatgc aactgataga
     1081 gtatcacctc ccccatgcta tcctctcgca aatgctaaat tcagcatttc aataccaaaa
     1141 gaatataatt tttcaccata ccatccgctc attaaaaaaa agcaaatact tacgcacaat
     1201 aatttcacgc caaatggcaa cgtctgttct tgcaactgcg ttaccgtttg aaaaagccct
     1261 accaataaac caacaaatgt cgctactgca ataggtcctg cagacataac taaaatcaga
     1321 tacagcgccc tattccctgc aaaaacaatg tcatccatat ttttttcctc agggattaat
     1381 ggataggtca aaatattgta atatgagtcc ttttgataac attgtccaac cgtccatcgc
     1441 gacaaacaga attaatttta tcggtgtaga tatggttacc ggactcatca tcatcatacc
     1501 taacgttaac agaacgctgg atataaccag atccactaca acaaagggta aatagatata
     1561 aaaacctata ataaatgcac tttttatctc acttaacgca tatgccggga gtaacgagaa
     1621 aattgaaata ttatcatcat cgattatctc ttcattgtct tcagaactat tcaccttctg
     1681 aatcttttca aaaaagctaa ccaactccgg ttcagaatat tttatcaaat atgatttata
     1741 accactcatg cctgtctcaa caaaattaac aaccgacgct acattattga atgaaagatt
     1801 ctcattctga ctgttataat atatttcctt cccaaccggc atcattacaa acattgacaa
     1861 tagcaaagca accccattaa gtgtcatatt tgatggcact tgctgaagtc ccaatgcgtt
     1921 tcggacaata acaaaaacaa tcgagaattt aataaaacat gtcccggatg ctattataaa
     1981 cggcaatagt gtaaataatg atagtatcgc tatcagcgaa atgctatttg acattattat
     2041 gccctgataa ccaagaatga atctctacgc ccaatttatc atccacttca acaagttcgc
     2101 catagccagt tagcgcgcca tttacacgta tctctatatt tttctcagat tcaggaagca
     2161 gagatattat tcttt
//
LOCUS       DAZKIP010000279         2160 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZKIP010000279 DAZKIP010000000
VERSION     DAZKIP010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.283"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..260)
                     /locus_tag="ACHOUK_004917"
     CDS             complement(<1..260)
                     /locus_tag="ACHOUK_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscC/YscC/HrcC family type III secretion system
                     outer membrane ring protein"
                     /protein_id="HFP9900164.1"
                     /translation="MKKISFFIFTALFCCSAQAAPSSLEKRLGKSEYFIITKSSPVRA
                     ILNDFAANYSIPVFISSSVNDDFSGEIKNEKPVKVLEKLSKL"
     gene            complement(260..715)
                     /gene="sepD"
                     /locus_tag="ACHOUK_004918"
     CDS             complement(260..715)
                     /gene="sepD"
                     /locus_tag="ACHOUK_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE switch protein
                     SepD"
                     /protein_id="HFP9900165.1"
                     /translation="MNNNNGIAKNDCDWLTALDFVKDVNGSPTHLTFYIYQKNAFLHD
                     FGNYWVLYIELSGDFRQVPTDTFIRLCNILAVSNEYKQMGIFLSNKKWYLCQIFHKDN
                     NHRANMSKAIMQHTLASLLDKQFDKLEQLSSSDTMMPPTHLFSDIGRIV"
     gene            complement(721..1293)
                     /gene="escJ"
                     /locus_tag="ACHOUK_004919"
     CDS             complement(721..1293)
                     /gene="escJ"
                     /locus_tag="ACHOUK_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscJ"
                     /protein_id="HFP9900166.1"
                     /translation="MKKHIKNLFLLAAICLTVACKEQLYTGLTEKEANQMQALLLSND
                     VNVSKEMDKSGNMTLSVEKEDFVRAITILNNNGFPKKKFADIEVIFPPSQLVASPSQE
                     NAKINYLKEQDIERLLSKIPGVIDCSVSLNVNNNESQPSSAAVLVISSPEVNLAPSVI
                     QIKNLVKNSVDDLKLENISVVIKSSSGQDG"
     gene            complement(1296..1673)
                     /gene="sctI"
                     /locus_tag="ACHOUK_004920"
     CDS             complement(1296..1673)
                     /gene="sctI"
                     /locus_tag="ACHOUK_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HFP9900167.1"
                     /translation="MNNINQSENINIQLNKAPQTNFVDEHTSLASAPSAAGAAQFLDQ
                     LLPKTAGVSSPEQVLIEEIKKRHLATMNSDLSFDALSAGGLSPEDVLTLQKNVLNANV
                     NVDVVSKLASLLSTSVTKLVSMQ"
     gene            complement(1757..2056)
                     /gene="espZ"
                     /locus_tag="ACHOUK_004921"
                     /gene_synonym="sepZ"
     CDS             complement(1757..2056)
                     /gene="espZ"
                     /locus_tag="ACHOUK_004921"
                     /gene_synonym="sepZ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE cytoprotective
                     effector EspZ"
                     /protein_id="HFP9900168.1"
                     /translation="MEAANLSPSGAVMPLATSLSGNNSVDEKTGVIKPENGTNRTVRV
                     IAGLALTTTALAALGTGIAAACSETSSTEYLALGITSGVLGTLTAVGGALAMKYA"
BASE COUNT          605 a          415 c          415 g          725 t
ORIGIN      
        1 tatagtttgg acagtttttc cagtactttt acaggttttt cattttttat ttcgccagag
       61 aaatcatcat tcacggaact actgatgaaa acaggaatgc tgtagttagc agcaaagtca
      121 ttaaggattg cacgtactgg agaactttta gtaataataa aatattcgct cttgcctaat
      181 cttttttcca gagaggacgg ggcagcttgt gcactgcagc aaaatagtgc tgtaaaaata
      241 aaaaaactta tttttttcat tacacaattc gtcctatatc agaaaacaag tgtgttggcg
      301 gcatcattgt atccgatgaa gataattgct ctagtttatc aaattgctta tcgagcaaag
      361 aggcgagagt atgttgcatt attgcttttg acatgtttgc gcgatgatta ttatctttat
      421 gaaaaatttg gcaaagatac cacttcttat tagacaggaa aatacccatt tgtttatatt
      481 cgttactaac ggccaggata ttgcagagtc tgataaatgt atctgttgga acctgacgaa
      541 aatcaccact aagctcaata tatagaaccc agtagtttcc aaaatcgtgc aagaaagcat
      601 ttttttggta gatataaaaa gtgagatgcg ttggtgaacc gttgacatcc tttacaaaat
      661 caagggccgt tagccagtca caatcattct ttgctatgcc attattattg ttcatacata
      721 ttacccgtcc tgtcctgagg atgacttgat aacaacacta atattttcta atttgagatc
      781 gtcaacacta ttcttaacta ggttttttat ctgaatgacc gatggtgcta agttaacctc
      841 tggcgagctg atgactaaaa cggctgctga ggagggttga ctttcgttat tgttaacatt
      901 taatgaaacg ctgcaatcga taacaccagg tattttactt agcaaccttt cgatgtcttg
      961 ttctttgaga tagttgattt ttgcattttc ttgtgatggc gatgccacta actggctagg
     1021 tgggaaaata acctcgatat ctgcaaattt ctttttcggg aagccgttat tatttaaaat
     1081 cgttattgcg cgaacaaaat cctctttttc cacactgagt gtcatatttc cacttttgtc
     1141 catttctttg gaaacattga catcattgga tagcaacagt gcttgcatct gattagcttc
     1201 tttttctgtt aatcctgtat acaattgctc tttacatgcc actgtcaaac aaatagcagc
     1261 caataaaaaa aggtttttaa tgtgtttttt catttttatt gcatcgaaac taattttgta
     1321 accgatgttg aaaggaggct tgctaactta gatacgacat caacgttgac gtttgcgttg
     1381 agcacattct tttgtaaggt gagcacgtct tctggcgaga gcccacctgc agatagagca
     1441 tcgaaactga gatcgctgtt cattgttgca agatgtctct ttttaatttc ttcaatcaac
     1501 acttgttctg gagaagacac tcccgctgtt ttagggagta attgatcaag aaactgagcc
     1561 gcacctgccg cagagggggc tgaggctaac gaggtatgct catcaacgaa atttgtttgg
     1621 ggcgctttat ttaattgaat attaatgttt tccgattgat taatgttgtt catatttact
     1681 gttagcctct ttttatgaaa gaggcaaggg cataatacat ccattacctc tatcttttat
     1741 ttatgcgatg ggccggttag gcatatttca tcgctaatgc accgccaacc gcagtaagag
     1801 tacctagtac gccagaagta atacccaggg ctaagtattc tgtgctgctc gtctccgagc
     1861 atgccgctgc aatacctgta cctagagctg ccagagccgt agtggtaagt gctaatccgg
     1921 ctataactct aacggtgcga tttgttccat tttctggttt aatcactcct gtcttctcat
     1981 ccactgagtt atttccactg agtgaggtcg ccagcggcat tactgcacca gaaggactta
     2041 aatttgctgc ttccattgat ctttctcctt ttgtctaatt tagacattta cctggttata
     2101 aataaccaaa ttatcccata ggcaactatt atattattta aatagttgca atgtacataa
//
LOCUS       DAZKIP010000280         2144 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZKIP010000280 DAZKIP010000000
VERSION     DAZKIP010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.284"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..1488)
                     /locus_tag="ACHOUK_004922"
     CDS             complement(<1..1488)
                     /locus_tag="ACHOUK_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFP9900169.1"
                     /translation="MNISNSQIDILRRDVRAGLRALFRPEPQTAVEWADASYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLSLAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEYYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYG"
     gene            complement(1463..2008)
                     /locus_tag="ACHOUK_004923"
     CDS             complement(1463..2008)
                     /locus_tag="ACHOUK_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFP9900170.1"
                     /translation="MKVNKKKLAEIFNVDPRTIERWQSQGLPCVSGGGKGVESVFDTA
                     TAIQWYAQREADIENEKLRKEVEDYRAASEADLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSD"
BASE COUNT          494 a          613 c          515 g          522 t
ORIGIN      
        1 tccgtagacg gatgcccctt taatggggat cacccggaac agcccatgtt ttttcgagcg
       61 ttcatacaca atggtcgggt caatcccgcc agtatcccag cagatacggg ataccgacat
      121 ttctgcacca ttccggcggg tataggtttt attgatggcc tcatccacac gcagcagcgt
      181 ctgttcatcg tcgtggcggc ccataataat ctgccggtca atcagccagc tttcctcacc
      241 cggcccccat ccccatacgc gcatttcgta gcggtccagc tgggagtcga taccggcggt
      301 caggtaagcc acacgatcag gaacgggcgc tgaataatac tctttccgct ctgccatcac
      361 ttcagcatcc ggacgttcgc cgattttcgc ttcccacgtc tcaccgagcg tggtgttcac
      421 gaaggtttta cgttttcccg tatccccttt cgtcttcatc cagtctttga caatctgcac
      481 ccaggtggtg aacgggctgt acgccgtcca gatgtgaaag gtcacactgt caggcggttc
      541 aatctcttca ccggatgacg aaaaccagag aatgccatca cgggtccaga tcccggtctt
      601 ttcgcagata taacgggcat cagtaaagtc cagctcctgc tggcggatga cgcaggcatt
      661 atgctcgcag agataaaaca cgctggaggg gtcatccggc gtccatttga ggccaaacgg
      721 cgtctctttg tcgccaaatt taagatactg ctcctccccg cagtgcgggc aggcaacatg
      781 aaaacgcata aaatgcgggg attcactggc tgcacgctca atctggcagg tgcctctcac
      841 tttgggcgtg gagccacgga tggactttgg ccagaccgag ccttcaatac gcttgtcacc
      901 caggaacgtc ggagagcctt cctgttcaat atcctcatca aaggcagcaa gttcatcata
      961 acccgccaca tcaaccgact tttcacggta gttttttgcc gctttaccgc ccaggcacca
     1021 gaagccacga ccattggtga aacgcttcat agtgagcgtg ttatcccggt gctttttgcc
     1081 ataccacggg gccagagaca gcagcgacgg aatatcgcgg atggtaggct cgacgtgggt
     1141 tttcataaag ttctcggcat caccatccgt cggcaaccag ataagggtgt tacgctgctt
     1201 atgctctatg aagtaggcat aaacacccag cagcattttt gaataaccaa cacgggcaga
     1261 cttcaccaca ttcacctcgc ggatgtagtc gctgcccatc gcattcatga tagcccgctg
     1321 aaagggtagt gtttcccagc gcccttcctg gtatgcggat tctttcggga gatagtaact
     1381 ggcatccgcc cattcaacgg cagtctgtgg ctccggcctg aacagggctc gcagcccggc
     1441 gcgtacatca cgccgcagaa tatcaatctg actgttcgat atattcactc agcaaccccg
     1501 gtatcagttc atccagcgcg gctgctttgt tcatggcttt gataatatcc cgtttcagga
     1561 aatcaacatg tcggttttcc agttccggaa aacgccgctg tactgagaga gggatcccgt
     1621 caagaatact ggcaatttca cctgcgatcc gtgacagcac gaaagtacag aatgcggttt
     1681 ccaccacttc tgcggagtct ctggcattct tcagctcctg ggcgtcagcc tgcgcacgcg
     1741 taagtcgatg gcgttcgtac tcaatagtcc caggctggag atctgcctcg ctggcagccc
     1801 tgtaatcctc aacctcttta cggagttttt cattttcgat atcagcttcc ctctgcgcat
     1861 accactgaat tgccgtggcg gtatcaaata cagattcaac gcccttacca cctccggaga
     1921 cgcaagggag cccctgagac tgccagcgtt caatcgttcg cggatccacg ttgaaaattt
     1981 cggcaagttt ctttttatta accttcatga aacagtctca caacaaatac agggtccgac
     2041 atgaaagtgc ccgaaaatga ctttttcagg cgttttcatg tcggaccttt acggattcga
     2101 tattagaaaa aacaaatagt tatgttcgag aagtaccgac atga
//
LOCUS       DAZKIP010000281         2132 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZKIP010000281 DAZKIP010000000
VERSION     DAZKIP010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.285"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..203)
                     /locus_tag="ACHOUK_004924"
     CDS             complement(<1..203)
                     /locus_tag="ACHOUK_004924"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900171.1"
                     /translation="MCRKYAHVVDALQGNFPYATDPAGNRLPDPELHPDSTLTAWPDN
                     RIAEDAHYVYRYDEYGRLAEKTDR"
     gene            complement(361..1011)
                     /locus_tag="ACHOUK_004925"
     CDS             complement(361..1011)
                     /locus_tag="ACHOUK_004925"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFP9900172.1"
                     /translation="MTQAAFLGETEVVSNLIKLGVDLDARGDLGRTALNEAVSNGYFD
                     IVKLLVESGADLTIKDEFGKTALELAEVHQNYKIVEWLSHYRDHNPIPDFSVLINIYG
                     ERYFGGEIIDTDARTELGEGVLHIAVRKRRQLDVRCFIQHGADINAKANNGFDFTPLH
                     YSIGIGDFDMMKLLLKLGADIYLESEMGYSPMDLAILMGDKRIIFYLYGYISHVSE"
     gene            complement(1218..>2132)
                     /locus_tag="ACHOUK_004926"
     CDS             complement(1218..>2132)
                     /locus_tag="ACHOUK_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP9900173.1"
                     /translation="RLERELRQGSVSEESQQWLAQCGLTAEQMAAQLEAEYIPERKLH
                     LYHCDHRGLPQALISPEGETAWQGEYDEWGNLLGETSAQHLQQPYRLPGQQYDEESGL
                     YYNRHRYYDPLQGRYITQDPIDIKGGWNLYSYALNPVSWIDPLGLTQCDSEGCNNDIL
                     FTGGSGPDNKILNELGPRDGIDGLGSQNMKMYSGLLGGDILKPGILGGLTLGSVTQRP
                     SRTAEEEVQAQIEYLAYKKRCEQKPDSNLSRCAAAIFQKSRKEDCLRMRQEWDDKWWP
                     GKHADEIKNVKASLKNTLIDVKKKMHAN"
BASE COUNT          563 a          569 c          360 g          640 t
ORIGIN      
        1 cggtccgtct tctccgccag cctgccgtat tcatcgtagc ggtagacata gtgcgcatct
       61 tccgcgatgc ggttatccgg ccatgccgtg agggtgctgt ccgggtgcag ttccgggtcc
      121 ggcagccggt ttcctgccgg gtctgttgca tacgggaaat tcccttgcag ggcgtccaca
      181 acatgtgcat atttcctgca catgctgtgg ttttgtcata caaggagcaa caatactagt
      241 taatgattca taaaaaattt taacggggtg atttttcatg cgattatcgt cctaattaac
      301 gccacctcac ttacagatta tttcattcgt tacaatcttt tgattgcatt acacccaggc
      361 ttattcagaa acatgagaaa tataaccata taaataaaaa ataattctct tatcccccat
      421 caaaatagcc aaatccatag gggagtatcc catctcactc tccaaataaa tatcagcacc
      481 taatttcaac aaaagcttca tcatatcaaa atcccctatc cctatcgaat aatgcaaagg
      541 agtgaagtca aagccattat ttgccttagc atttatatcg gcaccatgtt gaataaaaca
      601 tctgacatct aattgtcttc ttttcctaac agctatatga agaaccccct cgccaagttc
      661 tgtccttgca tctgtatcta taatctctcc accaaaatat cgctcaccgt agatattaat
      721 aagtacagaa aaatcgggta tgggattatg atctctataa tgtgatagcc attcaactat
      781 tttataattc tggtgtacct cagccaattc taatgccgtt ttcccaaatt catcctttat
      841 agtaagatcg gctcctgatt ctactaaaag cttaacaata tcaaaatacc cattacttac
      901 ggcctcatta agtgcggtac gccccaaatc cccacgagca tccagatcca cacccaattt
      961 aattaaatta cttacaacct cagtctcacc taaaaatgca gcctgagtta taggataata
     1021 atttaatttt gacttaaggt ggtaattttt cggcttaaaa aaaaatcagg caattcatca
     1081 ctatagcctc ccccccattg gcttcctcca taaatcggca gcatattaaa gataagaaaa
     1141 tatattatag cattttcacc aatcaatggg cataattaac atcattatat tttaaggata
     1201 cctatctcgg aatgccacta gttggcatgc atcttttttt taacatcaat aagtgtgttt
     1261 ttcaacgatg ctttcacatt ttttatttca tccgcatgtt tgccaggcca ccatttatca
     1321 tcccattctt gtctcattct taaacaatct tcttttctgc tcttttgaaa gatcgcggca
     1381 gcacaacgac ttaaattact atcaggtttt tgctcacaac gcttcttata tgctaaatat
     1441 tctatttgag cttgcacctc ctcctcagcc gttcgcgatg gtcgttgtgt cacactacca
     1501 agcgtcaaac cgcccagaat ccccggtttt aaaatatcgc cccctaatag cccactatac
     1561 attttcatgt tttgtgaacc caaaccgtca atgccatctc tcggacctaa ttcattaagt
     1621 attttattat cgggaccact tcctcctgta aataatatat cattattaca cccttcggaa
     1681 tcgcactgcg tcaatcctaa tgggtcaatc caacttaccg gattaagcgc ataagaatat
     1741 aaattccatc ctccttttat atctattgga tcctgggtga tatacctccc ctgtagcggg
     1801 tcatagtacc ggtgacggtt gtaatacagc cccgactcct catcatactg ctgtcccggc
     1861 agacggtacg gctgttgcag gtgctgcgcg ctggtttcgc ccagcaggtt tccccactcg
     1921 tcatactccc cctgccacgc cgtttcccct tccgggctga tgagcgcctg cggcagtccc
     1981 cggtggtcgc agtggtaaag atgaagtttc cgctccggga tatattccgc ctccagctgc
     2041 gcggccatct gttccgccgt cagcccgcac tgcgcaagcc actgctggct ttcttcactc
     2101 acgctgccct gccgcagttc ccgctccagc ct
//
LOCUS       DAZKIP010000282         2129 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZKIP010000282 DAZKIP010000000
VERSION     DAZKIP010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.286"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..260)
                     /locus_tag="ACHOUK_004927"
     CDS             complement(<1..260)
                     /locus_tag="ACHOUK_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFP9900174.1"
                     /translation="MNNCIFFSISASAVTARQFQRELDLADKAILWALISASTKEGRK
                     ACSLSYFACKAAEAELGLAYMAANDNKEFLTSLSNIMRYKIDA"
     gene            complement(895..1356)
                     /gene="yibG"
                     /locus_tag="ACHOUK_004928"
     CDS             complement(895..1356)
                     /gene="yibG"
                     /locus_tag="ACHOUK_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFP9900175.1"
                     /translation="MKACLLLFFYFSFICQLHGADVKIKQNESMMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYYCFTKNNIYKQLRYLEKSASQGNVTAQFNYGVFLSD
                     TNPTLSEYYNLNRAIYWMEFAVNNGNIDAKSKLQELKKLKRMDRRKNKENP"
     gene            complement(1368..>2129)
                     /locus_tag="ACHOUK_004929"
     CDS             complement(1368..>2129)
                     /locus_tag="ACHOUK_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFP9900176.1"
                     /translation="LISAEGATEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEES
                     GLYYNRHRYYDPLHGRYITQDPIGLKGGWNFYQYPLNPVINVDPQGLVDINLYPESDL
                     IHSVADEINIPGVFTIGGHGTPTSIESATRSIMTAKDIAYLIKFDGNYKDGMTVWLFS
                     CNTGKGQNSFASQLAKELHTNVIGPDTLWTWWGRGTNGKLKMDTVLTAPTNLNSNKDL
                     MAITTKDLGNWITYGPSGHPISNMQGTPEKPSDIR"
BASE COUNT          600 a          464 c          368 g          697 t
ORIGIN      
        1 gcatctattt tgtatctcat tatattagat aaggatgtga gaaattcctt gttatcattc
       61 gcagccatat atgccagtcc gagttcagcc tctgcggcct tacaggcaaa atagcttagt
      121 gaacacgctt ttcgtccctc tttagtactg gctgatatta gtgcccataa tattgctttg
      181 tctgccaaat ctaactctct ctgaaattgt cttgcagtaa ctgcactcgc agaaatagag
      241 aaaaaaatac aattgttaat aaccttgcca tattttttag tccgtaactg ttaatgtgct
      301 atctggctct gaaaatatta actcatttaa tttttgcatg tcattagata aaccagttat
      361 acaaccctcg gaacagaatc gaggtcctaa agacggacca aaagcgttta aacaactatg
      421 tattgaaaat ccattgcgat tataagttcc tgtattaact gacggtacta atcgtatgcc
      481 gtcaggtttt ctattgcttg ctcccgggtc attaacattc caactccata ggccttgaga
      541 tattggccat ctatttataa atgtgtacaa cggggattat ttttacattg catattatta
      601 ccagaagcga ctggaatact aagcacatca taaaaattat catcacatgg tatatctgaa
      661 acacacgaaa gcatacctaa agttatgctg tataaacagg tggctaatcc caaggggatc
      721 gattccagaa atcgggttca acggatactg ataaaaattc cacctacctt ccagcccaat
      781 cggagcctga gtgttatacc gcccctgctg cggttaatgt taccgataat agttatagta
      841 cagccctgat tcatcatcat actactgctg cggcagtctg atgaactatt gcttttacgg
      901 attctcctta ttcttccttc tatccattcg ctttagcttt ttgagctctt gaagtttgct
      961 ctttgcatca atattaccgt tatttacagc gaattccatc caataaatag ctctattcaa
     1021 attataatat tctgataatg ttggatttgt atctgataaa aagaccccat aattaaactg
     1081 cgctgtaaca ttcccctgag atgctgattt ttctaagtat cgcaattgtt tgtaaatgtt
     1141 atttttagta aagcagtaat attgatatag tcgaaatgat gcctctgaat caccatgttg
     1201 tgatttatac ttcagtttca tcaacttttc ttcacttaaa tcataggtca ttgctgtaga
     1261 ccccatcata ctttcgtttt gttttatttt cacatcagca ccatgcaatt gacaaataaa
     1321 agagaaataa aaaaatagta acaagcatgc tttcatacat ctacaaccta tcttatatca
     1381 ctgggttttt ctggcgtacc ttgcatatta gaaatggggt gcccagatgg cccatatgtt
     1441 atccaattac caaggtcttt tgttgttata gccattagat ccttatttga attaaggttc
     1501 gttggtgctg ttagcactgt atccattttt aacttaccat tagttcctcg cccccaccac
     1561 gtccatagcg tgtcaggtcc tattacattt gtatgtaact ctttagctaa ttggctagca
     1621 aatgaatttt gtcctttacc tgtattacaa gaaaataacc aaactgtcat cccatcttta
     1681 taattcccat caaatttaat tagatatgct atatctttag ctgtcatgat actgcgcgtt
     1741 gcggattcaa tagatgtggg ggtaccatgc cccccgattg tgaaaacgcc tgggatatta
     1801 atctcatcag ctacagaatg gataagatca ctttcggggt ataaatttat atcaaccaaa
     1861 ccttgcggat ctacatttat gaccggattc aacggatact gataaaaatt ccatcccccc
     1921 ttcagtccaa tcggatcctg agtgatatat cgcccgtgca gcgggtcata atagcggtgg
     1981 cggttgtaat acaggccgga ctcctcatca tactgctgcc ccggcaggcg gataagctgc
     2041 tgcagctgat gcgggttctc ttcattcagc aggttgcccc attcatcgta ttctgcgcac
     2101 cattctgttg ccccttccgc actgataag
//
LOCUS       DAZKIP010000283         2124 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZKIP010000283 DAZKIP010000000
VERSION     DAZKIP010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.287"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            45..320
                     /locus_tag="ACHOUK_004930"
     CDS             45..320
                     /locus_tag="ACHOUK_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspO"
                     /protein_id="HFP9900177.1"
                     /translation="MPFSIKNRFSSSQVHYPEISGPIKDKPASKNCILTSTTCNVDSY
                     TVYQKKACSFDMRPPGAGERTPKLKLSVTEMTWLSKTIETEIHNTKE"
     gene            complement(400..513)
                     /locus_tag="ACHOUK_004931"
                     /pseudo
     CDS             complement(400..513)
                     /locus_tag="ACHOUK_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308873.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="non-LEE encoded effector protein NleB"
     gene            complement(548..766)
                     /locus_tag="ACHOUK_004932"
                     /pseudo
     CDS             complement(548..766)
                     /locus_tag="ACHOUK_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            858..2086
                     /locus_tag="ACHOUK_004933"
                     /pseudo
     CDS             858..2086
                     /locus_tag="ACHOUK_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
BASE COUNT          610 a          499 c          539 g          476 t
ORIGIN      
        1 cggaatagca taaaaacact tttcatggag caaaggagaa aacaatgcca ttttcaatca
       61 aaaacagatt ttcaagttca caagtacatt acccggaaat atccggtccc ataaaagaca
      121 agccagcgtc aaagaactgc atacttacat caacaacatg taatgtagat agctatacag
      181 tgtaccaaaa aaaagcctgt agttttgaca tgcgcccacc cggcgcagga gaaagaaccc
      241 caaaactaaa actctcagtt actgagatga catggctatc taaaactata gaaacagaga
      301 tacacaacac aaaagaatag caaccactta ccagagaaca caaaaagcca cagacccgaa
      361 acacctgact gcaagcaacc acctccacag gaggtggttt taccatgaac ttcctgtgta
      421 ctgacttgtg ttcataataa tatttttgtg tttaaactca ataaagtcac aaaaatgatt
      481 gtaatcatgc aatgtagtaa aattaaaata tttgaccctg tacacgattc tgtgtaaatg
      541 ccttttctca gaagtgaccg tccaggcggt caccgaactc gataataaag cggctcattg
      601 ccatacgcca gtcccgcagt ggcatcgtcc atttctgtga agccgcctgg attgccagcc
      661 acacgacctt tttcactgag tcatccgtcg ggaacacctt gcgttttttg atggcatgcc
      721 ggattacgct gttcagcgac tcgatggcgt tggttgtgta gatgactaga ctggccccct
      781 gaatctccag acaaccagta tcacttaaat aagtgatagt cttaatacta gtttttagac
      841 tagtcattgg agaacagatg attgatgtct tagggccgga gaaacgcaga cggcgtacca
      901 cacaggaaaa gatcgcaatt gttcagcaga gctttgaacc ggggatgacg gtctccctcg
      961 ttgcccggca acatggtgta gcagccagcc agttatttct ctggcgtaag caatactagg
     1021 aaggaagtct tactgctgtc gccgccggag aacaggttgt tcctgcctct gaacttgctg
     1081 ccgccatgaa gcagattaaa gaactccagc gcctgctcgg caagaaaacg atggaaaatg
     1141 aactcctcaa agaagccgtt gaatatggac gggcaaaaaa gtggatagcg cacgcgccct
     1201 tattgcccgg ggatggggag taagcttagt cagccgttgt ctccgggtgt cgcgtgcgca
     1261 gttgtacgtc attctcagac gaaccgatga ctggatggat ggccgccgca gtcgtcacac
     1321 tgatgatacg gatgtgcttc tccgtataca ccatgttatc ggagagctgc ccacgtatgg
     1381 ttatcgtcgg gtatgggcgc tgcttcgcag acaggcagaa cttgatggta tgcctgcgat
     1441 caatgccaaa cgtgtttacc ggatcatgcg ccagaatgcg ctgttgcttg agcgaaaacc
     1501 tactgtaccg ccatcgaaac gggcacatac aggcagagtg gccgtgaaag aaagcaatca
     1561 gcgatggtgc tctgacgggt tcgagttctg ctgtgataac ggagagagac tgcgtgtcac
     1621 gttcgcgctg gactgctgtg atcgtgaggc actgcactgg gcggtgacta ccggcggctt
     1681 caacagtgaa acagtacagg acgtcatgct gggagcggtg gaacgccgct tcggcaacga
     1741 tcttccgtcg tctccagtgg agtggctgac ggataatggt tcatgctacc gggctaatga
     1801 aacacgccag ttcgcccgga tgttgggact tgaaccgaag aacacggcgg tgcggagtcc
     1861 ggagagtaac ggaatagcag agaacttcgt gaaaacgata aagcgtgact acatcagtat
     1921 catgcccaaa ccagacgggt taacggcagc aaagaacctt gcagaggcgt tcgagcatta
     1981 taacgaatgg catccgcata gtgcgctggg ttatcgctcg ccacgggaat atctgcggca
     2041 gcgggcttgt aatgggttaa gtgataacag atgtctggaa atataggggc aaatccagat
     2101 ggatttatcg taataaagac aaca
//
LOCUS       DAZKIP010000284         2050 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZKIP010000284 DAZKIP010000000
VERSION     DAZKIP010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.288"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(54..980)
                     /locus_tag="ACHOUK_004934"
     CDS             complement(54..980)
                     /locus_tag="ACHOUK_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9900178.1"
                     /translation="MMKIEPSILPSLAWFALIVRAGSFSRAASEMGITLAALSQNLKS
                     LEERLNTKLIYRTTRNISLTEEGQRLYEVLVSALGQIDDALKDVGDTQLEPTGLLRIN
                     SSRVAARMLVEPHIGEFLTRYPKTKIELIMDDGLSNIIADGCDVGIRLEQGLDEHMTA
                     VPVSPLIKLVTVASPDYLKEHGIPETPQELSNHNCLRLRHKSSGALSAWEFSKVVGGN
                     EEFEIEVSGKYISNDDESMIRMALNGTGIIQHLDFAIAEHINAGKLQPILEDWAVSFP
                     GFYIYVSSRVRMPSKVRAFIDFMVEKRVKLEN"
     gene            1137..>2050
                     /locus_tag="ACHOUK_004935"
     CDS             1137..>2050
                     /locus_tag="ACHOUK_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9900179.1"
                     /translation="MKTVSIKHTYWDIAADIYFPDDFNSEKKYPAIISAHPIGSCKEQ
                     TSGSVYGAALAKAGFIVIAFDASFQGSSGGEPRYLEDPTMRVKDFSIVVDYLTSLPYV
                     DAGRIGVLGICGGGGYAINAAMTERRIKAIGTVTGANYGRLMREGFTAFNPIGALEAM
                     AQQRTDEANGAKLRVDDLLPSSPDAALEAGLTEIDLFEATEYYRSPRGYAPNGVNRSL
                     FSHQTVAVGWDAFHLAEVLLTQPIMVVVGDRVGAFGAYRDGCEIIGRAASKHKELVVV
                     EGYSHYDLYDKPEPVKQALDKLIPFYKT"
BASE COUNT          602 a          467 c          435 g          546 t
ORIGIN      
        1 cgttattcct cttaccgata ttcgcccaga gcaaccatca attacacata tcatcaattt
       61 tcgagtttta ctcttttctc caccataaaa tcaataaaag cacgaacttt agagggcatt
      121 ctcacccgtg atgaaacata aatataaaac ccaggaaatg aaacagccca atcctcaaga
      181 atcggctgta acttgcccgc attgatatgt tcagcgattg caaaatccag atgttgaatg
      241 atacccgttc cattcagcgc cattcgtatc atgctttcgt catcattaga aatatactta
      301 cctgaaacct ctatttcgaa ctcttcatta ccccccacaa ccttagaaaa ctcccatgca
      361 gagagtgcac cgctactttt atgccgcaac cgaaggcaat tatggttact cagttcctga
      421 ggcgtttcag gaataccgtg ttcttttaaa taatctggcg atgccacagt aaccagttta
      481 ataagcggag aaacaggaac ggcagtcata tgctcatcca gtccttgctc aagacgtatg
      541 cccacatcac agccgtccgc aataatgtta gataaaccgt catccataat gagttctatc
      601 tttgttttgg ggtaacgagt aagaaactcg ccaatatgag gctcaacaag catccgtgct
      661 gcaacacggg aggaatttat tctcaacagg cctgtaggtt caagttgggt atcaccaaca
      721 tctttcaaag catcatcaat ttgccccaaa gccgacacca gcacttcata cagacgctgt
      781 ccttcctcag taagcgagat attgcgggtc gtacggtata tcagcttggt gttaagacgt
      841 tcctcaagag attttaaatt ctgggacaat gcggccagcg taatacccat ttcagaagcg
      901 gctctggaaa aactgccggc acgaacaatc agcgcaaacc aggcaagaga aggcaaaatt
      961 gaaggctcta ttttcatcat tgtaaacctt aacttaacac tgtgatagat attagaccat
     1021 tgatgataac caatacaagt catactatta cgccgttctc gatatgaaca caaatgccag
     1081 ttgcggataa tagaaatgat atttaaatct gtttaggcaa atccccggag cttgaaatga
     1141 aaactgtttc gataaaacac acttactggg atatagctgc tgatatctat ttccctgacg
     1201 attttaatag tgaaaaaaag tatcctgcca ttatcagcgc ccatccaatt ggaagctgca
     1261 aagaacagac atcgggtagt gtgtatggtg ccgccctggc aaaagctggc tttatcgtca
     1321 ttgcttttga tgctagtttt cagggaagca gcggtgggga accgcgttat ctggaagatc
     1381 caacaatgcg agtaaaagac ttcagcattg tcgttgatta ccttaccagc ttaccttacg
     1441 ttgatgccgg gcgtattggt gtactgggta tttgtggtgg aggtggatat gccattaatg
     1501 ccgcaatgac agaacgccga attaaagcta ttggtactgt cacgggtgca aactacgggc
     1561 gtcttatgcg tgagggattt acagcgttta atcctatcgg agcacttgaa gcaatggcgc
     1621 aacaaagaac agacgaagct aacggcgcaa aacttcgcgt agatgattta ctcccctctt
     1681 ctccggatgc agctcttgaa gcgggactaa cagaaataga cctttttgaa gccacagaat
     1741 attatcgttc gccacgaggc tatgcaccta atggggtaaa tcgttcgcta ttctcacatc
     1801 agactgttgc cgtgggttgg gatgcatttc atcttgcaga agtgctgtta actcaaccaa
     1861 taatggttgt ggtgggcgat agagtgggcg catttggtgc ctaccgtgac ggctgcgaaa
     1921 ttattggccg cgctgcttca aaacataaag agctggtcgt ggttgaaggt tattcacatt
     1981 acgacctgta tgataagcca gaacctgtga aacaggcact cgataaatta attccttttt
     2041 ataaaacaca
//
LOCUS       DAZKIP010000285         2034 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZKIP010000285 DAZKIP010000000
VERSION     DAZKIP010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.289"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            83..430
                     /gene="tnpB"
                     /locus_tag="ACHOUK_004936"
     CDS             83..430
                     /gene="tnpB"
                     /locus_tag="ACHOUK_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9900180.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            480..2018
                     /locus_tag="ACHOUK_004937"
     CDS             480..2018
                     /locus_tag="ACHOUK_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc8 family transposase"
                     /protein_id="HFP9900181.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYRQSEIYGRQGVELRRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKGIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLSRHSELAKAFAYALNQWPALTYYANDGWVEIDNNIAENALRAVSLGRKN
                     FLFFGSDHGGERGALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSELLPWRIA
                     LPAE"
BASE COUNT          453 a          565 c          613 g          403 t
ORIGIN      
        1 taacgttccg gcacgggacg ctccgcttca atggcaatgt cagcgaaaag ctcctgactc
       61 tgctgataca ggaactgaag cgatgatccc gttaccttcc gggaccaaaa tttggctggt
      121 tgccggtatc accgatatga gaaatggctt caacggcctg gctgcgaaag tacaaacggc
      181 gctgaaagac gatcccatgt ccggccatgt tttcattttc cggggccgca gcggcagtca
      241 ggttaaactg ctgtggtcca ccggtgacgg actgtgcctc ctgaccaaac ggctggagcg
      301 tgggcgcttc gcctggccgt cagcccgtga tggcaaagtg ttccttacgc aggcgcagct
      361 ggcgatgctg ctggaaggta tcgactggcg acagcctaag cggctgctga cctccctgac
      421 catgctgtaa atctctttat cctggttgtc acagaataag cccggtaaaa tacgggctta
      481 tgaacgacat ctcttctgac gacatcttcc tgctgaaaca gcgcctggcc gaacaggaag
      541 cgctgatcca cgccctgcag gaaaagctga gcaaccggga gcgcgaaata gaccatctgc
      601 aggcgcagct ggataaactc cgccggatga acttcggcag tcgttccgaa aaagtctccc
      661 gccgtatcgc acaaatggaa gccgatctga accggcttca gaaagagagc gatacgctga
      721 ctggtagggt gtatgacccg gcagtacagc gtccgttgcg tcagacccgc acccgtaagc
      781 cgttccctga atcactaccc cgtgacgaaa agcgactgtt gcctgcggcg ccgtgctgcc
      841 cgaactgcgg cggttcactg agctatctgg gcgaggatac cgccgaacag ctggagttga
      901 tgcgtagcgc cttccgggtt atccggacgg tacgggaaaa acatgcctgt actcagtgcg
      961 atgccatcgt gcaggcacct gcaccttcgc ggcccatcga gcggggtatc gccggaccgg
     1021 ggctgctggc ccgcgtgctg acctcgaagt atgcagagca caccccgctg tatcgccagt
     1081 cagaaatata cggccggcaa ggtgtggagc tgaggcgttc actgctgtcg ggctgggtgg
     1141 atgcatgctg ccggctgctg tctccgctgg aagaggcgct tcatggctat gtcatgactg
     1201 acggcaaact ccatgccgat gataccccgg tccaggtact gctgccgggt aataagaaga
     1261 cgaagaccgg gcggttgtgg gcgtatgttc gtgatgaccg caatgcaggg tcagcgttgg
     1321 cacctgcagt gtggttcgct tacagcccgg acagaaaagg catccatccg cagactcatc
     1381 ttgcctgctt cagcggtgtg ctgcaagcgg atgcgtacgc cgggttcaac gagctgtatc
     1441 gcaatggtgg gataacggaa gctgcctgct gggctcatgc ccgccgaaag atccacgatg
     1501 tgcacgtccg catcccgtca gcactgacgg aagaagccct ggagcagatc ggtcagttgt
     1561 acgccataga ggcggatata aggggaatgc cggcagagca gcggcttgct gaacgtcagc
     1621 gaaaaacgaa accgttgttg aaatccctgg aaagctggtt gcgtgaaaag atgaagaccc
     1681 tgtcgcgaca ctcagagttg gcgaaggcgt tcgcgtacgc acttaaccag tggccggcac
     1741 tgacgtacta tgcgaacgat ggctgggtgg aaatcgacaa caacatcgct gaaaatgccc
     1801 tgcgggcggt cagtctgggt cgtaaaaact tcctgttctt cggctctgat catggtggtg
     1861 agcggggagc gctactgtac agcctgatcg ggacgtgcaa actgaatgac gtggatccag
     1921 aaagctacct tcgccatgtg cttggcgtca tagcagactg gccggtcaac cgggtcagcg
     1981 aactgcttcc gtggcgcata gcactgccag ctgaataaca catccccgtc aata
//
LOCUS       DAZKIP010000286         2024 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZKIP010000286 DAZKIP010000000
VERSION     DAZKIP010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.290"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..384
                     /locus_tag="ACHOUK_004938"
     CDS             <1..384
                     /locus_tag="ACHOUK_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFP9900182.1"
                     /translation="LAEFVALISESGANPFGLTVDAVMEEYRRWRNESWRYDGSDKYP
                     WPQPVLYHICLEMRDRGIERQMTEGELKRLAERQLTKWAKQVGNGMSIPPIRRQLASP
                     KCPQGPTPIELLKQEYERRKAAGFV"
     gene            418..1134
                     /locus_tag="ACHOUK_004939"
     CDS             418..1134
                     /locus_tag="ACHOUK_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFP9900183.1"
                     /translation="METVSDALKALKRASSHVVAARLGISREEAVNELWKLKRRGEAD
                     NKGAIWWLTQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGCLIRVNQDGKFRYCIPGVDLPAEPKAASVAETEGKALPQPAGVALPVGEAE
                     TQEEIKTESVAVTVQSQPSFTRKHPDGLILPSLHVANRELRRAKGQVQKWERVCAALR
                     ELNKCRDILRDITATREQQR"
     gene            1167..1448
                     /locus_tag="ACHOUK_004940"
     CDS             1167..1448
                     /locus_tag="ACHOUK_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP9900184.1"
                     /translation="MILRQCAGTMKVKSVGALIGRTEAAVRTKARELGISMMLRGDFH
                     PSAKYSQRDIELARQLHQRGMQRREIARKLGMPLRIVNNYVYFDRRVSA"
     gene            1445..1672
                     /locus_tag="ACHOUK_004941"
     CDS             1445..1672
                     /locus_tag="ACHOUK_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900185.1"
                     /translation="MKILYQDYGPVGQVVISSTVMEFRKHNRVVDAVLLTCPGISASR
                     AGVFIMKTKLYGSKAWIKKAYRVALQEVNSE"
     gene            1665..1976
                     /locus_tag="ACHOUK_004942"
     CDS             1665..1976
                     /locus_tag="ACHOUK_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900186.1"
                     /translation="MSEIKEMPVVRDGYGYWTHPEYEKFCDGREYISTEEFNAWMEEN
                     NLQYVLCFRDEGCADLDACDADISAWEPERPEGDGWFIGSIHDTEDGPVCVWLRNKAE
                     A"
BASE COUNT          550 a          404 c          635 g          435 t
ORIGIN      
        1 ctggctgagt ttgtggcgtt gatttcggag agcggggcaa atccatttgg cctgacggtg
       61 gatgccgtga tggaggagta ccgtcgctgg aggaatgaat cctggcggta cgacgggagt
      121 gataaatacc cgtggccaca gccggtgctg taccacattt gcctcgagat gcgtgacaga
      181 gggattgagc ggcagatgac ggaaggtgag ttaaaacgac ttgcagaacg gcaactgacg
      241 aaatgggcaa agcaagttgg taacgggatg agtattcccc cgatccggag acaactcgca
      301 tcgccgaagt gcccgcaagg gccaacgcca attgaattac tgaaacagga atacgagcgc
      361 cggaaggcgg ctggttttgt ttgaatctga gaaacgattt tgtcggagga aattttaatg
      421 gaaaccgtat ctgacgcact gaaagcactg aaaagagcct cttcacatgt ggtggcagct
      481 cgccttggaa ttagtcgtga agaggctgtc aacgaactgt ggaaactgaa acgccgtgga
      541 gaagcggata acaagggggc gatatggtgg ctgactcagg ctggtgaaag tgaaccggtg
      601 tcaccggtac cgaaagtgac ggcgcaaatg ctgacagagg cgattgaaca acatggccca
      661 caaacggcgg atgaactggc attgatgttc ggaattacct cccgccgggc gaattcatcg
      721 ctggcaatgg caatcagcaa agggtgtctg attcgcgtga atcaggatgg taaatttcgt
      781 tactgcatac cgggcgttga tttaccggca gagccgaaag cagcatccgt agctgaaacg
      841 gagggtaaag cccttcctca gccagctggt gttgcgttac cagtcgggga agcggaaaca
      901 caggaagaaa taaaaacgga aagtgtggcg gtcacagtgc agtcacagcc gtcgttcacc
      961 agaaagcatc cggatggtct gattttacca tcgctgcatg tggctaaccg cgagctgcgc
     1021 cgggcaaaag gtcaggttca gaagtgggag cgagtctgcg ccgcgctgcg ggagctgaac
     1081 aagtgccggg atattctccg ggatattacc gccaccagag aacagcagcg gtgagtgggt
     1141 ggaagacgtg gtgccgggcg gaaatcatga tactccggca gtgtgcggga acgatgaagg
     1201 taaaaagcgt tggcgcactt atcggacgaa ctgaagcggc agtgagaacg aaggcacggg
     1261 agctgggcat cagcatgatg ttacgtggtg attttcaccc gtcggcaaaa tattctcagc
     1321 gtgatattga gctggcgcgg caactgcatc agagaggcat gcaaagaagg gaaattgcca
     1381 gaaaattagg catgccgctg cgcatagtga ataactacgt ttatttcgac aggagggtgt
     1441 ctgcgtgaaa atcctgtatc aggattacgg cccggtgggg caggtggtta tcagcagtac
     1501 tgtaatggag tttcggaagc ataaccgtgt ggtggatgct gtgctgttaa cctgtccggg
     1561 gatatcggcg agtcgtgcag gtgtgtttat tatgaagacg aaattatatg gcagtaaggc
     1621 gtggataaag aaggcgtatc gtgtagcgtt gcaggaggtt aacagtgagt gaaattaaag
     1681 aaatgccggt agttcgtgac ggatatggct actggacaca tcctgaatat gaaaaattct
     1741 gtgatggtcg ggaatatatt tcaacggaag agtttaacgc ctggatggag gaaaataatc
     1801 ttcaatacgt cctctgcttc agagatgaag gatgtgctga ccttgatgcg tgtgatgctg
     1861 atatttctgc atgggaaccg gaacgaccag agggcgatgg ctggtttatt ggttccattc
     1921 atgatacgga agatggcccg gtttgtgtct ggttgcgaaa taaggctgaa gcataaaggc
     1981 gataaaccaa ctaacaacta aatactgaag atttaaatca gaaa
//
LOCUS       DAZKIP010000287         2000 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZKIP010000287 DAZKIP010000000
VERSION     DAZKIP010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.291"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            85..1227
                     /locus_tag="ACHOUK_004943"
     CDS             85..1227
                     /locus_tag="ACHOUK_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFP9900187.1"
                     /translation="MTNKHPSLFSPFMLTEKIKLRNRIVMAPMTTWSANPDGTISEQE
                     LEFYKRRSQNVGLVITGCTYVTPSGIGFTHEFAAYDDRFINSLEKLAAAAKSGGAPAI
                     LQIFHAGNKAIPELVPNNDVISASASSVKSGDFMKRVVQSREMTENEIQETIRAFGDV
                     TKRAIKAGFDGIELHGAHGFLLQNFFSPLFNQRNDRWGGDLEGRMRFPLAVLQEVKNV
                     VYEYATKPFAIGYRISPEESATGGLRIEDTYKLLDRLISSGISYIHTSLVSINDSYPV
                     ESPNGPRTIELILNHIAGRVPVIAAGKIRTPSQAQEAISTGLPLVAIGKGLVINPEWV
                     TLAESGRGHEIQTTLNPQLVPELTIPDKLWDQIQASKGTGWFPLMD"
     gene            1701..1801
                     /gene="ykgR"
                     /locus_tag="ACHOUK_004944"
                     /pseudo
     CDS             1701..1801
                     /gene="ykgR"
                     /locus_tag="ACHOUK_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YkgR"
BASE COUNT          621 a          431 c          407 g          541 t
ORIGIN      
        1 ccagaactct tggttccaaa gctggcaaca actaaccagt taaaacatac gcatcaaaat
       61 cagataaaca tttgaggtat tgttatgaca aataaacacc catcattgtt ttctcctttt
      121 atgctgacag aaaaaataaa gctacgtaat cgtatcgtta tggctcctat gacgacctgg
      181 tcagcgaatc ctgatggaac catctctgag caagaactcg aattttataa acgtcgctca
      241 caaaatgttg gtttagtaat aacaggatgt acctatgtca cgccaagcgg cattgggttt
      301 actcatgaat ttgccgcgta tgacgaccgt ttcattaaca gccttgaaaa gttagcagcg
      361 gcagccaaaa gtggcggtgc tccggctatt ttgcagattt tccacgccgg taataaagcc
      421 atcccggagc ttgtaccaaa taacgatgtt attagtgcca gcgcatcaag cgtaaaatct
      481 ggtgacttta tgaagagagt ggtacaaagc agggaaatga ctgaaaacga aattcaggaa
      541 accattagag cattcggaga tgtcacaaaa agggcgatta aagcaggttt tgatggcatt
      601 gaacttcatg gtgcgcacgg attccttcta cagaattttt tctcccctct atttaaccag
      661 cgtaatgacc gatggggagg agatctcgaa ggacgtatgc gcttcccgct ggccgtatta
      721 caggaagtaa aaaatgtagt ctatgaatat gcgacaaagc cctttgccat cggttacaga
      781 atatctcctg aagaatctgc aacagggggg ctgcggatag aggatactta taaactcctt
      841 gatcggctca tttcatccgg tatttcgtat atacatacct cgctggtcag tattaacgat
      901 agctaccctg ttgagtcgcc aaatggcccc cgaactattg aattgatttt aaatcatatt
      961 gcaggcagag tgccggtaat cgctgcaggt aaaatacgca caccgtctca ggctcaagaa
     1021 gcaatttcca ctggcctgcc acttgttgcg atcggtaaag ggctggtgat aaatccagaa
     1081 tgggtgactt tagctgaatc aggtagaggc catgaaattc aaacaaccct taatccacaa
     1141 cttgttcctg aattaaccat tccggataaa ctatgggatc aaattcaggc atcgaaagga
     1201 actggctggt ttccgttaat ggattaaata tctattactc tcccgagagt aaaactatca
     1261 aatcaatgag taaccgatac agcctcacaa attctttcag ataattaacc cgtcactgcg
     1321 gcacgttctc tcataaggcg tgcctgcagt gtaatctagt atgtatccgg catagttgac
     1381 ggtttaatag agctcaattc ctcgcccagg ttaccaacaa cgatatcttg tataacaccc
     1441 caggagatac gacattatga tgagatgcga tatgctcctc agcgccaatc aacgccagga
     1501 ctgggccagt cttgagatca gactcattcc cggaactgac ttcaggcgac atatatcaat
     1561 gacgttgggg caataaaaga tgcctgatgt aatacatcta ttcaaatttg tgatacatgt
     1621 caaaaaattg gttgaccaaa ctcgttattt atataagggc acttacgaag tgcgctcttt
     1681 tttaaagcga ggaagtacca atgaaagaga ataaagtaca acaaatcagt cataaactga
     1741 ttaatatcgt tgttttgtcg caattgtaga atacgcctat ttatttctcc atttctatta
     1801 ataacggcaa taaactgttc acttcagtga tatttaaaat atgcatcctc tccctttttg
     1861 taagtaatta ttatatccgt gggagaggaa tacacgctgt caggtaatca atcatactgc
     1921 gataaatcat cggccagtaa agtggagata acctccattc tcgaaaaatc catactctca
     1981 gcgaaactat catcaatcac
//
LOCUS       DAZKIP010000288         1964 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZKIP010000288 DAZKIP010000000
VERSION     DAZKIP010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.292"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(191..1090)
                     /locus_tag="ACHOUK_004945"
     CDS             complement(191..1090)
                     /locus_tag="ACHOUK_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9900188.1"
                     /translation="MKIDLNLLPIFIAVAEESNFSKAAARLGVTRSAVSQGIRRLEDA
                     FGTMLVMRTTRSVNLTEAGERLHKSLSLPLSGIEAAFEEMVSDHVPRGLLRIAVTSIA
                     EEFLSGPLIASFAAANPAVTLDIVVTDEEFDIVAAGYDAGVRLGEVIEKDMIAVPLTG
                     RQREMVVASPSYLAANSTPVHPRELVNHKCIGWRQSPEVAPYRWPFEENGRAFDLAIE
                     PQITTNDLRLMLRLALAGGGITIATQETFRPYIESGKLVSLLDDFLPQFPGFYLYFPQ
                     RRNIAPKLRALIDHVKEWRQQLA"
     gene            1189..1911
                     /locus_tag="ACHOUK_004946"
     CDS             1189..1911
                     /locus_tag="ACHOUK_004946"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9900189.1"
                     /translation="MDKVILITGASSGIGEGIARELGMTGAKVLLGARRVERIEAIAT
                     EICRAGGIAKARELDVTDRQSMADFVQAALDSWGRVDVLINNAGVMPLSPLAAGKQDE
                     WALTIDVNIKGVLWGIGAVLPVMEAQGSGQIINLGSIGALSVVPTGAVYCASKFAVRA
                     ISDGLRQESSKIRVTCVNPGVVESELASTITHVETMKAMDAYRSVALQPADIARAVRH
                     IIESPESVDTTEITIRPTASAN"
BASE COUNT          529 a          457 c          473 g          505 t
ORIGIN      
        1 tattttaatt tatgtattaa aatcattgtg ttggtggcga ttattggttc ctattatcgc
       61 accatttaaa tcaagtagtt accccatatt taaatacacc acgtttcctc ctgtgccgca
      121 tttgtgccat tgtaaccctg gcaattaatc aaaatactgt tctgacatca ggcaatgcag
      181 gtgcagacat ttaagccaat tgctgccgcc attccttaac gtggtcaatc agagcgcgga
      241 gctttggtgc aatattgcga cgctgtggga aatacagata gaagcccgga aattgtggaa
      301 gaaagtcatc aagcagagat acaagcttac cgctttcaat atacggcctg aaagtttcct
      361 gagtggcaat tgttattcct ccgccggcaa gagccagcct caacatcaga cgcagatcat
      421 tagtcgtaat ctgcggttca atcgcaaggt cgaaagctct cccgttttct tcaaatggcc
      481 agcgataagg cgcaacctcc ggggactgac gccagccgat acacttatga ttcacaagtt
      541 cacgcggatg aacaggtgtg ctgttggctg cgagatagga gggagaggca acgaccattt
      601 cacgctgcct gccggtaagg ggcacagcaa tcatgtcttt ttcaatgacc tctccgagcc
      661 ttacgccagc atcgtaacct gcggccacga tatcgaattc ctcatccgtg acaacaatat
      721 caagcgtaac ggctggattg gctgctgcaa acgaagcgat tagcggacct gaaagaaatt
      781 cttcagctat tgacgtcacc gcgattctga gaagtccgcg tggcacatgg tcagaaacca
      841 tctcttcgaa cgctgcctca ataccggata aaggtaatga cagcgattta tgcagccgtt
      901 ctcccgcttc tgtcaaatta actgagcgcg tggtccgcat tacaagcatt gtcccgaatg
      961 catcttcaag gcgtcttatt ccctgactga ccgctgaacg ggtaacgccc agccgtgcgg
     1021 cggctttact aaaattactc tcttctgcta ccgcgataaa aatgggtaaa agattaagat
     1081 cgattttcat tgtttaatgc cgctaaccac tgagtacatt gaaagcagga tactggaacc
     1141 aatcataaag ttctattctc tctccgaaca catttgaagg agaacgtaat ggataaggtc
     1201 atattaatta ccggcgcatc aagtggtatt ggggaaggta ttgccagaga gcttggaatg
     1261 acaggtgcaa aggttttact gggagcacgc agagttgagc gtatcgaagc cattgcaacc
     1321 gaaatctgcc gcgcaggagg aattgctaaa gcgcgggagt tggatgtcac agaccgacag
     1381 tccatggccg atttcgtgca agcagcgctg gatagctggg ggcgagttga tgttcttatc
     1441 aataatgcgg gcgttatgcc gctttcaccg cttgcagcag gaaaacaaga tgaatgggcg
     1501 ctcactattg acgtgaatat caaaggtgta ctgtggggaa ttggcgctgt acttccggtg
     1561 atggaagcac agggttccgg tcagataatc aatcttggtt ctattggtgc cctttctgtt
     1621 gtgcccacag gcgcagtcta ttgtgcatca aaatttgcag tacgggctat ttctgatggc
     1681 ttacggcagg aaagctcgaa aatccgtgtg acctgcgtaa atcctggcgt tgtcgaaagt
     1741 gaactggctt caaccattac ccatgtggaa accatgaagg caatggacgc ctaccgttct
     1801 gttgcacttc aacccgcgga tattgcccgt gctgtaaggc atattattga atctcccgaa
     1861 agcgttgata caaccgaaat taccatcaga cccactgcat ctgcaaactg aagaataact
     1921 tttacattca cgttttatta aataggtcag aaggaatatt tata
//
LOCUS       DAZKIP010000289         1926 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZKIP010000289 DAZKIP010000000
VERSION     DAZKIP010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.293"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..625
                     /locus_tag="ACHOUK_004947"
     CDS             <1..625
                     /locus_tag="ACHOUK_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFP9900190.1"
                     /translation="GSVLPENELEALRHIARSNQNDTITIGGRNMRLHYIQFMDGFSV
                     EPILGGLWDHLGAREAHHLADRLTRQLNGGNTFLQAYSLYLEQRQAAPLVQESVIKTL
                     LDRINSNAFPVSLQDFSCTEEHLNCPITLHIPETGVFVRNARNSEICALYDQEALTEL
                     ILRNALHPLSRDPFAPEMIISKDKCHFNITKQCFYALPIYPLQQNSI"
     gene            704..1762
                     /gene="espW"
                     /locus_tag="ACHOUK_004948"
     CDS             704..1762
                     /gene="espW"
                     /locus_tag="ACHOUK_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspW"
                     /protein_id="HFP9900191.1"
                     /translation="MPKISSVVSSCYHLFSEHQQLSNETTMTNPVSRRIVHKEYGISL
                     KSVPVWLATAKTPLALLNGRHTRSHSFIIAGTPGMGSRSGAQYYAINSDDKRSRIDID
                     SLFLKKLNNVRNQNKFPIDVKETVIKLQGQKFTCIEDFYKRYNETRLKANTNIQQEQI
                     ADEVKSLTYLIPSEKKEMWIYKNNGKDNAKPNLGERDVRMFENISSDDTDKITGRKFS
                     ELGEYLYSGNVIKLSQLSIRYLPNISSISLIETKQSLLLHRLYSDEVLQRNGTLIPTP
                     LHEEKSIPADNIKTMLNNIPTYKMLPPFTETQGNCSSGAATFLRKSGAEEKDILACSP
                     RNYGLHHNIKTWDPLVRN"
BASE COUNT          714 a          366 c          332 g          514 t
ORIGIN      
        1 tggaagtgta ttgccggaaa atgagctgga ggctttacga catatagcaa gaagcaacca
       61 gaatgacact attactatag gaggccgtaa tatgaggctt cattatatcc agtttatgga
      121 tggttttagt gttgagccta ttcttggtgg actttgggac catcttgggg caagagaggc
      181 tcatcatctc gcagaccgcc tgacaagaca acttaacgga ggtaacactt ttctgcaggc
      241 atacagctta tatctggagc agaggcaagc tgccccgctt gtgcaggaga gcgtcataaa
      301 aacactacta gatcgaataa attcgaatgc atttcccgtt agtttacaag acttttcctg
      361 tactgaggaa catcttaatt gtccgataac gctacatatt cctgagacag gtgtttttgt
      421 cagaaatgct cgaaattcag aaatatgtgc attatatgat caggaagcat tgactgaact
      481 tatcctgcgt aacgctctcc accccctcag ccgtgacccc tttgctccag aaatgattat
      541 aagcaaagac aagtgtcatt ttaatataac aaaacaatgt ttttacgcat tacccatata
      601 cccacttcaa caaaacagta tttaaaaata aaagcacaat ataagaagca tattttcaat
      661 aagttaatca ccatataaca aacatatagc gaggagacta tttatgccca aaatatcatc
      721 agttgtatca tcatgttacc atctgttcag tgaacatcaa caactttcaa atgaaacaac
      781 aatgacgaac cccgtctcca gaagaattgt tcataaagaa tatggtatat ctttaaaatc
      841 cgttcctgta tggttggcta cagctaaaac tccccttgct ctactcaatg gcagacatac
      901 aagaagtcac tcatttatta ttgcagggac cccaggaatg ggaagcagga gcggagccca
      961 atactatgcc ataaacagtg atgataaacg ctcccgaata gacattgact ctttattttt
     1021 aaaaaagtta aataatgtgc gaaatcaaaa taaatttcca atcgatgtaa aagaaacggt
     1081 tataaaacta caagggcaaa aattcacatg cattgaagat ttttataaaa ggtataatga
     1141 aaccaggtta aaggccaata ccaatatcca acaagaacaa attgcagatg aagtaaaatc
     1201 acttacatat ttgattcctt cagaaaaaaa agagatgtgg atatataaaa acaatggaaa
     1261 agataatgca aaaccaaact taggagagcg agacgtaaga atgttcgaaa atattagttc
     1321 tgatgataca gataagataa caggaaggaa attttcagag ttaggtgagt atctttattc
     1381 agggaatgta ataaaactca gtcagttatc aattcgttac ttacccaata tcagctcaat
     1441 ctcattaata gagacaaaac agagtttgtt gctacatcga ttgtattcag atgaagtact
     1501 tcagagaaat ggaacgctta tcccgacacc actacatgaa gaaaaatcaa ttccagctga
     1561 caatataaaa acaatgctca acaacatacc gacttacaaa atgttaccgc cattcacaga
     1621 aacacaaggt aattgttctt ctggcgcagc cacgttttta cgcaaatcag gcgccgaaga
     1681 aaaagatatt cttgcatgta gcccccgaaa ttatgggctg catcataaca taaaaacatg
     1741 ggaccccttg gttagaaatt aaggatccaa atattatata tttcatataa agcaagaaaa
     1801 aaatattgtc tgaataattg ttcagacaat atatccttac atggcactat tataataaac
     1861 tattaatata aaaacacacc aacaaaaaaa attaagcatc acttgcaaca aaggattctt
     1921 tttttg
//
LOCUS       DAZKIP010000290         1904 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZKIP010000290 DAZKIP010000000
VERSION     DAZKIP010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.294"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            218..>1904
                     /locus_tag="ACHOUK_004949"
     CDS             218..>1904
                     /locus_tag="ACHOUK_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HFP9900192.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVDEPDVLSDSKNENGLL
                     FDKRKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQEKLATPAGQQVAQSLQQTGDTRELL
                     DILADDVWLKNEYHGEDEIFTGLASLNDLVRLAAAMGTEFPFDEYAEVQKLPVIDVAF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEMMMRDQLQKASAVLAVMDYTQMNSKADE
                     EVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGALPVDEAWVDDFIREAFGPMVEEDDWKDSTKVNKKAEKL
                     WNISLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENVSEYLSLRHQGLQQSIQSL
                     QAHITSLLADIQEIEECQNQVTGDVRMAMEDINTKTGELLTKVCASLEEELNDYFRSG
                     KRKEQQMLEEENSAQPRERNAFAFFHDIFGTGNQHDRMRDFDPDSPEIKFSDRR"
BASE COUNT          537 a          451 c          520 g          396 t
ORIGIN      
        1 tccgggaaaa tatcaccctg ggcgttcagg ccggttatgc ccacagcgtc agcggcagca
       61 gcgctgaagg ctataacggt caggctacgc tgaatatgac tttctgatta tacggtatcc
      121 cggaaatacg atttcaggaa aattcatgca gtaatgggaa aggacgctca ttgctgttca
      181 ccgcagttct gaattattga atttataaag gtgaatgatg cacgaaaaga acatcgccct
      241 gctttgtgat gaagccgacc ggcttttgca actgaatatc aatctgctcc ggcaaatggt
      301 tgatgagccg gatgtgttat ctgacagtaa gaacgaaaac gggctgcttt ttgataaacg
      361 gaaagcgctg aaaagaattg aggagctgga gggcgaacaa atcaaaaccg cccgcaggga
      421 gatggtgctg gctgtggtcg gcacgatgaa ggcgggcaaa tcaaccacca tcaacgccat
      481 tgtggggcag gaaattctgc ccaaccgtaa ccgccccatg acctcggtac cgacattgat
      541 ccgccacgtt cccggaaaaa cggaaccggt cctccatctg gaacatattc agcccgtccg
      601 caatttatta atcacactgc aggaaaaact cgccaccccg gcagggcagc aagtcgcaca
      661 gtccctgcag caaaccgggg atacccgcga actactggat attctggcgg atgatgtctg
      721 gcttaaaaat gaataccacg gggaagacga aatctttacc ggactggcat cgttaaatga
      781 cctggttcgt cttgctgcgg caatggggac tgaatttcct tttgacgaat acgcagaagt
      841 gcagaaacta ccggtgatcg acgtggcgtt cagccacctg gtgggaatgg atgcatgtca
      901 gggaacactc acactgctgg atacccccgg ccctaatgaa gccggacaac cacagatgga
      961 aatgatgatg cgggatcaac tacagaaagc ctctgcggtg ctggcggtga tggattacac
     1021 ccagatgaac tcgaaagccg atgaggaggt tcgtaaagag cttaatgcca ttgctgacgt
     1081 ctcagccggt cgtctgtttg tgctggtcaa taaattcgat gaaaaagatc gcaatggtga
     1141 tggtgcggat gccgtacgcc agaaggtgcc agcaatgctg aacagcgatg tgctgcccgc
     1201 ctcccgcgtt tatcccggct catcacgtca ggcgtacctg gctaaccgtg cgcttcatga
     1261 gttacgaaaa aacggtgcgc ttccggttga tgaagcctgg gtcgatgatt ttatcaggga
     1321 agctttcgga cctatggtgg aagaggacga ctggaaagac agcactaagg taaataaaaa
     1381 ggcagaaaaa ctctggaata tatctctgat cgatcaactg ataacggaag tcatacagag
     1441 ctcccattcc agagcggcgg cactggcggt tgactctgcc gccgcaaaac tgatgcagaa
     1501 tgcagaaaat gtcagcgaat atctgtcatt acgtcatcag gggctgcagc aaagcattca
     1561 gtcactacag gcgcatatta ccagcctgct ggcggatatc caggagattg aggaatgtca
     1621 gaatcaggtg accggtgatg tcagaatggc catggaggac atcaatacca aaaccgggga
     1681 attactgacg aaggtctgtg cctcactgga ggaggagctg aacgactatt tcagaagcgg
     1741 taaacgcaaa gaacagcaaa tgctggagga agaaaattca gcgcagcccc gggagagaaa
     1801 cgcattcgct ttctttcacg atattttcgg aaccggaaat cagcatgaca ggatgcggga
     1861 ctttgatccg gacagccccg aaataaaatt cagtgatcgc aggg
//
LOCUS       DAZKIP010000291         1900 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZKIP010000291 DAZKIP010000000
VERSION     DAZKIP010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.295"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            143..1819
                     /gene="tir"
                     /locus_tag="ACHOUK_004950"
     CDS             143..1819
                     /gene="tir"
                     /locus_tag="ACHOUK_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocated
                     intimin receptor Tir"
                     /protein_id="HFP9900193.1"
                     /translation="MPIGNLGHNPNVNNSIPPAPPLPSQTDGAGGRGQLINSTGPLGS
                     RALFTPVRNSMADSGDNRASDVPGLPVNPMRLAASEITLNDGFEVLHDHGPLDTLNRQ
                     IGSSVFRVETQEDGKHIAVGQRNGVETSVVLSDQEYARLQSIDPEGKDKFVFTGGRGG
                     AGHAMVTVASDITEARQRILELLEPKGTGESKGAGESKGVGELRESNSGAENTTETQT
                     STSTSSLRSDPKLWLALGTVATGLIGLAATGIVQALALTPEPDSPTTTDPDAAASATE
                     TATRDQLTKEAFQNPDNQKVNIDELGNAIPSGVLKDDVVANIEEQAKAAGEEAKQQAI
                     ENNAQAQKKYDEQQAKRQEELKVSSGAGYGLSGALILGGGIGVAVTAALHRKNQPVEQ
                     TTTTTTTTTTTSARTVENKPANNTPAQGNVDTPGSEDTMESRRSSMASTSSTFFDTSS
                     IGTVQNPYADVKTSLHDSQVPTSNSNTSVQNMGNTDSVVYSTIQHPPRDTTDNGARLL
                     GNPSAGIQSTYARLALSGGLRHDMGGLTGGSNSAVNTSNNPPAPGSHRFV"
BASE COUNT          537 a          401 c          492 g          470 t
ORIGIN      
        1 tgcatcaaaa atcagattgt gatttatttg gtcgatgtca tgttcagccg tttatcgact
       61 acgtgctgta taccatacat tattatgtaa tagcaaaata aaggattgtt atctatttac
      121 ataaaataaa aaggagatat ttatgcctat tggtaatctt ggtcataatc ccaatgtgaa
      181 taattcaatt cctcctgcac ctccattacc ttcacaaacc gacggtgcag gggggcgtgg
      241 tcagctcatt aactctacgg ggccgttggg atctcgtgcg ctatttacgc ctgtaaggaa
      301 ttctatggct gattctggcg acaatcgtgc cagtgatgtt cctggacttc ctgtaaatcc
      361 gatgcgcctg gcggcgtctg agataacact gaatgatgga tttgaagttc ttcatgatca
      421 tggtccgctc gatactctta acaggcagat tggctcttcg gtatttcgag ttgaaactca
      481 ggaagatggt aaacatattg ctgtcggtca gaggaatggt gttgagacct ctgttgtttt
      541 aagtgatcaa gagtacgctc gcttgcagtc cattgatcct gaaggtaaag acaaatttgt
      601 atttactgga ggccgtggtg gtgctgggca tgctatggtc accgttgctt cagatatcac
      661 ggaagcccgc caaaggatac tggagctgtt agagcccaaa gggaccgggg agtccaaagg
      721 tgctggggag tcaaaaggcg ttggggagtt gagggagtca aatagcggtg cggaaaacac
      781 cacagaaact cagacctcaa cctcaacttc cagccttcgt tcagatccta aactttggtt
      841 ggcgttgggg actgttgcta caggtctgat agggttggcg gcgacgggta ttgtacaggc
      901 gcttgcattg acgccggagc cggatagccc aaccacgacc gaccctgatg cagctgcaag
      961 tgcaactgaa actgcgacaa gagatcagtt aacgaaagaa gcgttccaga acccagataa
     1021 tcaaaaagtt aatatcgatg agctcggaaa tgcgattccg tcaggggtat tgaaagatga
     1081 tgttgttgcg aatatagaag agcaggctaa agcagcaggc gaagaggcca aacagcaagc
     1141 cattgaaaat aatgctcagg cgcaaaaaaa atatgatgaa caacaagcta aacgccagga
     1201 ggagctgaaa gtttcatcgg gggctggcta cggtcttagt ggcgcattga ttcttggtgg
     1261 gggaattggt gttgccgtca ccgctgcgct tcatcgaaaa aatcagccgg tagaacaaac
     1321 aacaacaaca actactacaa ctacaactac aagcgcacgt acggtagaga ataagcctgc
     1381 aaataataca cctgcacagg gcaatgtaga tacccctggg tcagaagata ccatggagag
     1441 cagacgtagc tcgatggcta gcacctcgtc gactttcttt gacacttcca gcatagggac
     1501 cgtgcagaat ccgtatgctg atgttaaaac atcgctgcat gattcgcagg tgccgacttc
     1561 taattctaat acgtctgttc agaatatggg gaatacagat tctgttgtat atagcaccat
     1621 tcaacatcct ccccgggata ctactgataa cggcgcacgg ttattaggaa atccaagtgc
     1681 ggggattcaa agcacttatg cgcgtctggc gctaagtggt ggattacgcc atgacatggg
     1741 aggattaacg ggggggagta atagcgctgt gaatacttcg aataacccac cagcgccggg
     1801 atcccatcgt ttcgtctaaa tatatccata atcattttat ttagagggag ggagggggga
     1861 agtctaacta acgtcaattt ttcagggaat aacattgctg
//
LOCUS       DAZKIP010000292         1875 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZKIP010000292 DAZKIP010000000
VERSION     DAZKIP010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.296"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            1176..1778
                     /gene="fimB"
                     /locus_tag="ACHOUK_004951"
     CDS             1176..1778
                     /gene="fimB"
                     /locus_tag="ACHOUK_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFP9900194.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          564 a          328 c          393 g          590 t
ORIGIN      
        1 gtcataaaat agagtgtctg ttgatctcat tttcatattc ctttcaggct gaagtgcgtt
       61 ttcatcaccc gatgagaaag aactgaagtg atgtttgcca tagattgcaa gatgccctgt
      121 ctcattgtct atcgtggtgc acctttatac ctattatacc agatcgaaaa tcacgcaatc
      181 catacaacaa aaccagattt gcaattcgtg tcacaaaata tgtcgatctt tttctaagaa
      241 gaagatgcca tgtgaagcca gacgaacact tgcggtggtc ttcaaaaact aaagatcttc
      301 gtttaactat ttgttttata aataatttat ttagagtcta aacaagggaa gctttgcaag
      361 ctaactcagt gagcttggtg taaatcagtg tttacccgcc atcaggctga gcataattct
      421 catcatgaaa tatgtttcct ggtttgtggc ttgtaactgg tcacttctga agtcgatctg
      481 gagaggctta ttgatgttgg tgttttcagg atgatgtttc acttagtttg tttgccgtat
      541 cgcccggcga atggctgtga ttgaggaagg tttaagtcgt agtgaccaaa gctatattta
      601 ccaacgaatg tagatgaaaa aatcatctcc tgcgttcccc catatctcca ggataaaaag
      661 gaatgtaaca atctcatggc gtaagctgac gaatcagcca ggaataatcg ctagggacct
      721 aagaattagc ttgataatag ccactaagaa attactgtgt tccatgaaat agccattttg
      781 tggcaaatgg agttgactaa taatgtcata tgtgagacgg ctagttgaac gaatattaaa
      841 ttttgctgaa ttttttatgt tgattttact tgttacagaa catatcacat gatatataga
      901 taagattagt tgcattaatg atgagggtta ttattagatt cgtatccgat tgataaatat
      961 ataaaggtac atagcatgca agagcatagc gtttgtatgg caacgttatt ataattaact
     1021 gttgctactc catttaagtt cactcagaag aactggtcca cttacgttag ttattaagca
     1081 aacgttcgct tttataaaca taatcaggat aaaaatgttg gattattgct aacctagcac
     1141 agctagtgcg cgtctgtaat tataagggaa aaacgatgaa gaataaggct gataacaaaa
     1201 aaaggaactt cctgacccat agtgaaatcg aatcactcct taaagcagca aataccgggc
     1261 ctcatgcagc acgtaattat tgtctgactt tgctttgttt tattcatggt ttccgggcga
     1321 gtgaaatttg tcgattgagg atttcggata ttgatcttaa ggcaaagtgt atatatatcc
     1381 atcgattaaa aaaaggcttt tcaacaacgc acccgctatt gaacaaagaa gttcaggctt
     1441 taaaaaactg gttgagtatc cgtacttctt atccgcatgc tgagagcgag tgggtatttt
     1501 tatcgcgtaa ggggaatccg ctttctcggc aacaatttta ccatattatc tcgacttccg
     1561 gtggtaatgc cgggttgtca ctggagattc atccgcacat gttacgccat tcgtgtggtt
     1621 ttgctttggc gaatatggga atagatacgc gacttatcca ggattatctt gggcatcgca
     1681 atattcgtca tactgtctgg tatactgcca gcaatgcagg gcgtttttac ggcatctggg
     1741 atagagccag aggacgacag cgtcacgctg ttttatagag atagttgaga taccagggat
     1801 ggtgttaaaa actacattat tcttacgata tatcaaaaat gattaaccat ttattaattg
     1861 atgttattgt aagta
//
LOCUS       DAZKIP010000293         1874 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZKIP010000293 DAZKIP010000000
VERSION     DAZKIP010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.297"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..897
                     /locus_tag="ACHOUK_004952"
     CDS             <1..897
                     /locus_tag="ACHOUK_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP9900195.1"
                     /translation="RLERELRQGSVSEESQQWLAQCGLTAEQMAAQLEAEYIPERKLH
                     LYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGETSAQHLQQSLRLPGQQYDEESGL
                     YYNRNRYYDPLQGRYITQDPIGLEGGWNLYQYPLNPIEHIDPLGLALDLNYYSPSDPI
                     YKGSLNVREFPTGFTVGGHGSPTSMSDDRIKKGSDLTIKQLASDIRANPKYHEGMPVV
                     LFSCETGKGKNSFAQKLANELDATVIAPDEIIWIWPDGNYAIMGQTARITIGGKDNGV
                     FELVPDEKQPGDFHKFTPTGSK"
     gene            966..1517
                     /locus_tag="ACHOUK_004953"
     CDS             966..1517
                     /locus_tag="ACHOUK_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFP9900196.1"
                     /translation="MDNSKHSDEAEKLLAELSARKGEGEGEGEGEGEGEGEGEGEPKS
                     TVSVFYLQPEEVNTLSHQAKRGDGEAGFRLFLYYKLSNYDEEKSSQWLKIAANNGHAT
                     AQYRLYVELQEQGETKEANEWLQKIKLAASNNDSSAIWFLVNCYKNQGNKKEALQWAY
                     KLKASKQPLDADRLIEELTKEQK"
BASE COUNT          609 a          337 c          485 g          443 t
ORIGIN      
        1 aggctggagc gggagctgcg gcagggcagc gtgagtgaag aaagccagca gtggcttgcg
       61 cagtgcgggc tgacggcgga gcagatggcc gcgcagctgg aggcggaata catcccggag
      121 aggaaacttc atctttacca ctgcgaccac cggggactgc cgctggcgct catcagcccg
      181 gaaggggaaa cggcgtggca gggggagtat gacgagtggg gaaacctgct gggcgaaacc
      241 agcgcgcagc accttcaaca gtcactccgt ctgccggggc agcagtatga tgaggagtcg
      301 gggctgtact acaaccgcaa ccggtactat gatccgttgc aggggagata tatcacccag
      361 gacccgatag ggctggaggg gggatggaac ctgtatcagt acccactcaa tcctattgaa
      421 catatagatc cgttggggtt agcacttgat ttgaattatt attctccgtc agatcctata
      481 tataaaggtt ctcttaatgt aagggaattt ccaacgggtt ttacagttgg aggtcatggt
      541 tcgcctacat ctatgagtga tgacagaatc aaaaaaggaa gtgatctgac aataaaacaa
      601 ttagctagcg acataagagc gaatcctaaa tatcatgaag gtatgcctgt agtcttgttt
      661 tcctgcgaaa caggaaaagg caaaaattca tttgcacaga aactagctaa cgaacttgat
      721 gctacagtga ttgctcctga tgaaataata tggatttggc cggatggaaa ctatgctatt
      781 atggggcaaa cagctaggat aacaataggc ggtaaggaca acggggtgtt tgaattggtg
      841 ccagacgaga aacaaccagg ggactttcat aagtttacac ctacaggaag caaatagtaa
      901 tgaaagtatt tacttttttg ttgattatca tggtatgtct ttataacttt ggagtgaggg
      961 ctgctatgga taactcaaaa cattctgatg aggcggaaaa attattggcg gagctttctg
     1021 ctagaaaagg tgaaggtgaa ggtgaaggtg aaggtgaagg tgaaggtgaa ggtgaaggtg
     1081 aaggtgaacc aaaatcaaca gtaagcgtct tttatttgca gccagaagaa gtaaatactt
     1141 tatcacacca ggcaaaacga ggcgatggcg aggcgggttt taggttgttc ttgtactaca
     1201 agttgtccaa ttatgatgaa gaaaaaagta gtcagtggtt aaaaatagcg gctaataatg
     1261 gtcatgcgac agctcagtat cggttatatg tagagttaca agaacaaggt gaaaccaagg
     1321 aagctaatga atggttacaa aaaattaaat tggctgccag taataatgac agttcagcaa
     1381 tatggttctt ggtgaattgt tataaaaacc agggaaataa aaaagaagca ttgcaatggg
     1441 catataaact caaagcgagt aaacagccgc ttgatgcaga tcgactaatt gaagaattaa
     1501 cgaaagaaca aaagtagctt taaacaggta acagaaaaat ggagagtaga aaaactttac
     1561 cagtcgctac aacacctaca cacaaaatta cgtttacgac gactgcaaca agctcatcaa
     1621 agcgcaagtt gaaaccaaaa ctggcatctt cgtcgccggt tatcattacg atgtacttgg
     1681 cagatgcatc aaaagtcgca agagaaaaga tgaaaatata gctttttgta tcattaagta
     1741 tgtggccagc acatgactcg gcatagatct tatgactaag attctacacc ccaaacctaa
     1801 gtatgggcca aagaagccgt ttggttcatt ttttatatct ccagcatttt agtggatgga
     1861 gcttatgaaa ataa
//
LOCUS       DAZKIP010000294         1861 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZKIP010000294 DAZKIP010000000
VERSION     DAZKIP010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.298"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..330
                     /locus_tag="ACHOUK_004954"
     CDS             <1..330
                     /locus_tag="ACHOUK_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein O"
                     /protein_id="HFP9900197.1"
                     /translation="TAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRN
                     HRDMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGMIASKPKLDLTNTD
                     WIYGVDL"
     gene            327..1028
                     /locus_tag="ACHOUK_004955"
     CDS             327..1028
                     /locus_tag="ACHOUK_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFP9900198.1"
                     /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVIAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELTCMTARINRGETIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            1025..1327
                     /locus_tag="ACHOUK_004956"
     CDS             1025..1327
                     /locus_tag="ACHOUK_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFP9900199.1"
                     /translation="MTGKEAIIHYLGTHKSFCAQDVAAVTGATVTSINQAAAKMARAG
                     ILVVDGKVWRTVYYRFATREEREGKVSTNLIFKECRQSAAMKRVLRVYKRTSMGTQ"
     gene            1395..1727
                     /gene="emrE"
                     /locus_tag="ACHOUK_004957"
     CDS             1395..1727
                     /gene="emrE"
                     /locus_tag="ACHOUK_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFP9900200.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAVIGMMLICAGVLVIN
                     LLSRSTPH"
     gene            1792..>1861
                     /locus_tag="ACHOUK_004958"
     CDS             1792..>1861
                     /locus_tag="ACHOUK_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900201.1"
                     /translation="MFLVFVYSFVPSRLLRKQIAIRL"
BASE COUNT          501 a          406 c          498 g          456 t
ORIGIN      
        1 acagcagaag acctgaccgc cgcagagtgg atgtttgaca tggtgaagac catcgcgcca
       61 tcagccagaa aaccgaattt tgcagggtgg gctaacgata tccgcctgat gcgtgaacgt
      121 gacggacgta accaccgcga catgtgcgtg ctgttccgct gggcatgcca ggacaacttc
      181 tggtccggta acgtgctaag tccggccaaa ctccgcgaca agtggaccca gctcgaaatc
      241 aaccgtaaca agcaacaggc aggcatgata gccagcaaac caaaactcga cctgacaaac
      301 actgactgga tttacggggt ggatttatga aaaacatcgc cgcacagatg gttaactttg
      361 accgtgagca gatgcgtcgg atcgccaaca acatgccgga acagtacgac gaaaagccgc
      421 aggtacagca ggtagcgcag atcatcaacg gtgtgttcag ccagttactg gcaactttcc
      481 cggcgagtct ggctaaccgt gaccagaacg aactgaacga aatccgccgc cagtgggttc
      541 tggctttccg ggaaaacggg atcaccacaa tggaacaggt taacgcagga atgcgcgtag
      601 cccgtcggca gaatcgacca tttctgccat cacccgggca gtttgttgca tggtgccggg
      661 aagaagcatc cgttatcgcc ggactgccaa acgtcagcga gctggttgat atggtttacg
      721 agtattgccg gaagcgtggc ctgtatccgg atgcagagtc ttatccgtgg aaatcgaacg
      781 cgcattactg gttggttacc aacttgtacc agaacatgcg ggccaatgcg ctgactgacg
      841 cggaattacg gcgcaaggct gccgatgaac tgacctgtat gacagcgcga attaaccgtg
      901 gtgagacgat acctgaacca gtaaaacaac ttcctgttat gggcggtaga cctctaaatc
      961 gtgcacaggc tctggcgaag atcgcagaaa ttaaagctaa gttcggactg aaaggagcaa
     1021 gtgtatgacg ggcaaagagg caattattca ttacctgggg acgcataaga gcttctgtgc
     1081 acaggacgtt gccgcggtaa caggtgcaac cgtaaccagc ataaatcagg ctgcggctaa
     1141 aatggcgcgg gcaggaatcc tggtcgttga tggtaaggtc tggcgaacag tgtattaccg
     1201 gttcgctacc agagaagaac gggaaggaaa ggtgagcacg aatctgattt ttaaggagtg
     1261 tcgccagagt gccgcgatga aacgggtatt gagggtatat aaaagaacat caatgggtac
     1321 acaatgatga aacaggtgag ttgagttcaa actgtagtac aattctctcc agtttgaaca
     1381 ggaaagaata tgttatgaac ccttatattt atcttggtgg tgcaatactt gcagaggtca
     1441 ttggtacaac cttaatgaag ttttcagaag gttttacacg gttatggcca tctgttggta
     1501 caattatttg ttattgtgca tcattctggt tattagctca gacgctggct tatattccta
     1561 cagggattgc ttatgctatc tggtcaggag tcggtattgt cctgattagc ttactatcat
     1621 gggggttttt cggccaacgg ctggacctgc cagccgttat aggcatgatg ttgatttgtg
     1681 ccggtgtgtt ggttattaat ttattgtcac gaagcacacc acattaaaaa taatttcttt
     1741 taaaagactg caatatggcg gttcatatct ttacatgggc cgcttttgtt aatgttttta
     1801 gtttttgtgt attcttttgt gccttcaaga ttattgcgta agcaaattgc aatacgatta
     1861 t
//
LOCUS       DAZKIP010000295         1854 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZKIP010000295 DAZKIP010000000
VERSION     DAZKIP010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.299"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..458)
                     /locus_tag="ACHOUK_004959"
     CDS             complement(<1..458)
                     /locus_tag="ACHOUK_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP9900202.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLREIDLTVQGGERYFFCNELNE
                     KGEAVTWQGRQYQVYPIDGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSELTAMTASFVLA"
     gene            complement(458..787)
                     /locus_tag="ACHOUK_004960"
     CDS             complement(458..787)
                     /locus_tag="ACHOUK_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9900203.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWTPPYAWRQIKVTCAAWSSRVRMLRVEFSA
                     EFKQVVN"
     gene            complement(784..1854)
                     /locus_tag="ACHOUK_004961"
                     /pseudo
     CDS             complement(784..1854)
                     /locus_tag="ACHOUK_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
BASE COUNT          358 a          679 c          422 g          395 t
ORIGIN      
        1 gccagcacaa acgaggctgt catggccgtc agctctgaca tctgctccac cacccagcgg
       61 tcgctcagct cctgctccgg gtccgcttcc ggattgcccg ccacaaaatt caccgcatcc
      121 agaaaacggg catacacccg gcggcggacc accgtggccc ccaccaggct ctgcaggtcc
      181 tccgccatcc cggtgaccag accaaacaga ttggacaccg tcagcgacgg tctggcactg
      241 ctgcccttcc cgttcatctc aaaaccgctg ccgtcaatcg ggtatacctg atattgccgc
      301 ccctgccagg tgaccgcctc ccctttttca ttcagctcat tacagaaaaa ataccgctca
      361 ccgccctgca ccgtcaggtc aatttccctg agcaccaccc gcggtgactg ctctgattta
      421 accgactcgt tcagactttc ttcgtgaata tcctgcatca gttcaccacc tgcttaaact
      481 cggcactgaa ttcaacccgc agcatgcgaa cccgtgatga ccaggcggca caggtcacct
      541 ttatctgccg ccaggcataa ggcggtgtcc acagaaacgc cttccagcca ccgtgctctg
      601 ccaggaatgc ctccagatgt cgggcctcct cccgggtcac ggaaagcgtc acacggtatg
      661 ttttcaggtc agcattcagc cccgccgcca tacgctgcga atacccgtca ccaaaacgca
      721 cttcacgcac cgatggctgc gagttcacct ccatatccgg cttcactttc cagcgaaatg
      781 ttttcatcgc ctgcctccgg aaaagacgcc gccatcacgc atctgcgcct gaatctcatc
      841 ctgcgccccc ttgcgggcca tgtcatacac cgccttcatc agctgcggcc ccgcctgtcc
      901 gttggagccg tcgttctgaa tcaccacgtg attgttctga ttaaaattaa tgccttccgt
      961 ccgccgcatc tgcgccggac ttccggcacc acccacataa ccaccttccg catagccccg
     1021 catcaggcgg tacaggttgc cgacgccaat ccggctggtt gcttccttcg tgaagacaaa
     1081 ctccccgcga tgaacaattc ccgcaggttc atatttaccc cccgtccccg taaatccccc
     1141 ggtcgcgaaa tggaagttcg ccgccgcagc ctgaatggcc gtccccgtgg aggcagacgc
     1201 accaccaccg aaagcaccgc caatggcgct gccgatactc ccgactatcc ccaccatcgc
     1261 ctgcttcaga aaaatctctg tcagcatgga cagcaccgaa cgggtgaatc cccgccagtc
     1321 tgcctctgca ccggtcagca tcgccgccat attctgtgca ataccgtcaa aggtctgcgt
     1381 ggcagcactt ttaacctgcg aaaaactgtc cgtcgcactt tccgcccact cgccccagcc
     1441 ggacttcagc ccggccatcc agcttccacg aagctgctcc tccgcagacc aggtgttctt
     1501 cagtgcagat gtggccttcg ccagcgcatc cggattatca ccgtacacgt cacgaaggcg
     1561 ctgctcttcc gactcccgct gcgcctgacg gtcggtgagg cccgcgcctg ggcactgatt
     1621 gccgcctgct tcgcgccctg ctgctgctca aaccgcgcag cctgctgtgc cagctcattc
     1681 agccgtttct ggtgttcaac tttgtctccc agctcagcca gctggcgttt gtactccagc
     1741 gtctcgtctt tatgcgccag cagggatttt tcctgctcag ataactgccg tttcgtggca
     1801 gcctctttca ggaccgcata ctgattttcc gctttccata aatcgcgacg ctgc
//
LOCUS       DAZKIP010000296         1852 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZKIP010000296 DAZKIP010000000
VERSION     DAZKIP010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.300"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..630)
                     /locus_tag="ACHOUK_004962"
     CDS             complement(<1..630)
                     /locus_tag="ACHOUK_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP9900204.1"
                     /translation="MVVVCSVIMVCSPINIFLEKDTLSLKPGSVVLATKCIRALFLMH
                     YGKVKIFDINHSIVSQYLEIQHKLTRTHLTDVPLYLSLEPNNPALAEALITSQRFSGD
                     TTDMFLMMACLSLFESDERILLFLSGCLSSISAKVRAIIQTDISASWTLGAIALRLHM
                     SESLLKIKLKNEGHMFSRLLLEERMRVAVNMLCSRHGYGQAVAEKCGYSS"
     gene            complement(768..964)
                     /locus_tag="ACHOUK_004963"
                     /pseudo
     CDS             complement(768..964)
                     /locus_tag="ACHOUK_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
     gene            complement(1251..>1852)
                     /locus_tag="ACHOUK_004964"
     CDS             complement(1251..>1852)
                     /locus_tag="ACHOUK_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFP9900205.1"
                     /translation="SAIALFFHLWPGFHNPVVLNSVTVGPQSTPYTMYFNFDKALVPF
                     LLVLCTSSLFKKEVKSEVSLWKWGALSLSVPLILFLAVFFGGLKPEIHFPEWLPEFIL
                     ANLFFVSLAEESLFRGYIQSRLSEVTSPLVALIVAALLFGFYHYSGGALLVLFATLSG
                     VVYGLSWMWSGRLWVATLFHFGLNLCHLLFFTYPFLKHN"
BASE COUNT          626 a          437 c          332 g          457 t
ORIGIN      
        1 gcttgaataa ccgcattttt ctgctacagc ctgtccatat ccatgccggg aacataacat
       61 attgacagca acacgcatcc gctcttccag caacaagcga ctgaacatgt gcccttcatt
      121 tttcagtttt atctttaaca aactctcact catatgcagg cgtaacgcaa tcgcaccaag
      181 cgtccagctt gctgatatat ctgtctgaat tatcgccctg actttggcac ttatactgga
      241 taaacatcca cttaaaaata ataatatccg ttcatctgat tcaaacagcg acaggcatgc
      301 catcataaga aacatatccg tggtatctcc ggaaaatctc tggctggtaa ttaaagcctc
      361 agccaacgca gggttgttgg gttccagtga cagataaagc ggaacgtcag tcagatgagt
      421 tcttgtcagc ttatgctgaa tttccagata ttgacttact atggaatggt ttatatcgaa
      481 aattttaact ttgccataat gcataaggaa aagcgccctg atgcatttgg tggccagaac
      541 gactgagccg ggcttaagtg acaacgtatc cttttcaaga aaaatattaa ttggggagca
      601 aaccatgata actgaacaga caacaaccat tataatttta ctttcattag caattggtta
      661 gttcaattat agccccaaaa ggtaaattat catcaacaca taagcaaagg actgacaggt
      721 gccgctaaca cccaccagcc gcccatttac cacaaataaa aaagccttca ggactgaagg
      781 cgtctgtaac aaccgaactg atagtctgcc agacccgcat aaccagctgg gtcagtatta
      841 actggcagcg ctcgcgtgaa aggtacgtat tctgcgcaat ctccccgacc gtcgccggtt
      901 cggtgacgct taattcatca aacacaactc tggcggtttc tgtcatatcc tgctgtttca
      961 gcatgtcttt ttaccctttc cggttaacgt gacacaccaa taactcttgt cgaaaaagcc
     1021 agcaagctga aagacctgta ttaataacta ccagcacatt taacgcactg cgctactttg
     1081 cgggcacaaa aaacccgctc agaggcgggg tcaagctatg cggcgaaata accactctta
     1141 acagcatacc tgatttttta cgtacgtaaa tgctttgccg tgcatatttt tcatgcaaat
     1201 gtcacgtcct gctatttttc agtcttataa atttaaaacc atagaaaaaa tcaattatgt
     1261 tttaaaaatg gataggtaaa gaataacaag tgacacagat tcaaaccaaa atggaaaagg
     1321 gtggcaaccc acaaacgccc actccacatc catgacaatc catacacaac accagataac
     1381 gtggcaaata atacaagtaa agcaccacct gaatagtgat aaaaaccaaa caacaaagcc
     1441 gccacaatta atgcaaccaa tggagacgtt acttctgata gccgtgattg aatataccct
     1501 ctaaataatg attcctctgc cagagacaca aaaaacaaat tagccaatat aaactctggc
     1561 aaccactcag gaaaatgaat ctctggcttt aatccaccaa aaaaaacagc caaaaacagg
     1621 ataagaggaa cagagagcga cagagccccc cacttccaca aagacacttc tgattttact
     1681 tcttttttaa acaaagaaga tgtacacagg actaacaaaa atggcaccag cgctttatca
     1741 aaattaaaat acattgtata gggagtactt tgagggccaa cagtaacaga atttagcact
     1801 acaggattgt gaaaccctgg ccatagatgg aaaaataatg ctatggctga ta
//
LOCUS       DAZKIP010000297         1840 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZKIP010000297 DAZKIP010000000
VERSION     DAZKIP010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.301"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..181)
                     /locus_tag="ACHOUK_004965"
     CDS             complement(<1..181)
                     /locus_tag="ACHOUK_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900206.1"
                     /translation="MWIVLVLSLSTLSWHKVVAFSLLTVSVVLAVLNDIIDWSVLFFV
                     ATIVFFIILKFNWKYN"
     gene            complement(333..704)
                     /locus_tag="ACHOUK_004966"
     CDS             complement(333..704)
                     /locus_tag="ACHOUK_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFP9900207.1"
                     /translation="MARDIQMVLERWGAWAANNHEDVTWPSIAAGFKGLIPTKVKSRP
                     KCSDDDAMIICGCMARLNKNNQYLHDLLVDYYVGGMTFMALARKHRCSDGLIGKRLYK
                     AEGIIEGMLMALNVRLDMDMR"
     gene            complement(694..1065)
                     /locus_tag="ACHOUK_004967"
     CDS             complement(694..1065)
                     /locus_tag="ACHOUK_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP9900208.1"
                     /translation="MRIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAEVLIDDEQFDEINIVRGQ
                     PVPGGRLGVKIYEIRGGNDGA"
     gene            complement(1078..>1840)
                     /locus_tag="ACHOUK_004968"
     CDS             complement(1078..>1840)
                     /locus_tag="ACHOUK_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP9900209.1"
                     /translation="HGDYHHSETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAQQN
                     LAAWMIDVIRHAMNGIQERELSLAELSWWAVCNQVVDALPEAVSRRSLGLPAEKIRSV
                     YRESDIIPGEQTATSILKQRTKNIALPPHTHQQQNPPQEKTVVSIAVDPESPKSFMKR
                     PKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHH
                     NELHADPLAFEEKHGSQVDLIFRFLDHAFATGVLG"
BASE COUNT          506 a          488 c          370 g          476 t
ORIGIN      
        1 cgttatattt ccagttgaac ttcaaaataa taaaaaaaac gattgtagca acaaaaaata
       61 acaccgacca atcaataata tcattaagca cagccaggac aacagacacc gtcaacaatg
      121 aaaaagccac taccttatgc caactgagag ttgacagtga cagtactaac acaatccaca
      181 taagcactcc ttttatttaa tgatgaagat tgattatcaa tattttcaat tcaccaggca
      241 acattttatc taccttccac aatacatgac catcaaaaaa taaacatgtt aattataaac
      301 acagaaaaca taaccctcat cactatatat ccctaccgca tatccatatc taaccggaca
      361 ttcagagcca taagcattcc ttcaataata ccttccgctt tataaagcct tttaccaata
      421 agcccatcag aacatctatg cttacgtgca agagccataa atgtcattcc acctacgtaa
      481 taatccacca acaaatcgtg caaatactga ttattcttgt ttaatcgtgc catacaacca
      541 caaattatca tggcgtcatc atcagaacac ttaggacgtg atttcacttt agtcgggatt
      601 aatcctttaa aaccagcagc tatcgagggc catgttacat cttcatgatt atttgctgcc
      661 catgcccccc atcgctcaag aaccatctgg atatcacgcg ccatcgttac cacctctgat
      721 ttcgtaaatc ttcacgccca gccgtccacc tggcacaggc tgaccgcgca caatattgat
      781 ttcatcaaac tgctcgtcat caatgagcac ttccgcatgc gtcagcgcat ccagcggtgc
      841 tttcagaatg ttgtccaggt cacggcggcg cttatccggt ggttctgcaa taatttttat
      901 tgccagccgt ccggacaggc ttaatttcag ccgctgctgg cgaacaataa gcgccacagc
      961 ccggcgataa cgctcaccgg cttttgatac aaaatatgtg ctgccacgac gtcgccagta
     1021 ggtgttcacc gtcggcgggt aaggcaaaac aaactctata cgcatcagta acctctttta
     1081 cccgagcacg ccggttgcaa aagcgtgatc aagaaaacga aaaattaaat caacctggga
     1141 accatgcttt tcttcgaacg ccagcggatc cgcatgaagc tcgttgtgat gctcccgaca
     1201 cagcggtagc gtgaaaatat cgtgggattt tgttcccatt ccaccctgac catgaccaat
     1261 cagatgatga ggatcgtccg ctggcttacc acaacacgca cacggctgtg tctttaccca
     1321 gcgtgtgtat ttctcattta cccagcggcg acgtttaggt cgtttcatga aggatttcgg
     1381 agactccgga tcaacggcaa tgctgaccac cgtcttttcc tgtggtgggt tctgttgctg
     1441 gtgggtgtga ggcggtagcg caatattttt tgtgcgctgc ttcagtatgc tggtggcggt
     1501 ctgttctccc ggtatgatgt cgctttcacg gtatacggag cggatttttt ccgccggtaa
     1561 tcccagagaa cgacgcgata ctgcctcagg taatgcgtcc accacctgat tgcagactgc
     1621 ccaccaggat aattcagcca gcgataattc ccgttcctgt atgccattca ttgcgtggcg
     1681 gatgacgtca atcatccagg cggccagatt ctgttgagca agttgctcaa gtgattcgga
     1741 tgtctggtcg cgcagctggt tgtcgcagtg ccagcacaac accatcgcgc cggtaccgta
     1801 acgatgtatg acagtttcgc tgtgatgata atcaccatga
//
LOCUS       DAZKIP010000298         1825 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZKIP010000298 DAZKIP010000000
VERSION     DAZKIP010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.302"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            345..539
                     /locus_tag="ACHOUK_004969"
     CDS             345..539
                     /locus_tag="ACHOUK_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900210.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            882..1010
                     /locus_tag="ACHOUK_004970"
     CDS             882..1010
                     /locus_tag="ACHOUK_004970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900211.1"
                     /translation="MGADVTEFSVQGKSGTCRQFSDLFNREIISYSLSERSVMEHR"
     gene            complement(1320..1655)
                     /locus_tag="ACHOUK_004971"
     CDS             complement(1320..1655)
                     /locus_tag="ACHOUK_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900212.1"
                     /translation="MVISTVGYEKRTGSASSASIIIQKELQPDNAIIQGKAISSVGRN
                     VEISTGKRGTDINTRRIPASNCLSARNAELKHNHTGARILWSYAFSIVTTNRNKNLAG
                     ANIKRCDTV"
BASE COUNT          567 a          403 c          375 g          480 t
ORIGIN      
        1 ccagaccacc aggctgatag ctcacagttt gttctccgat tcaataaaac ggataagaac
       61 catcagcgcg gtagcgtaag gcactacagc gcattgcttt acgagtaata acaatggatt
      121 tatcaccaga agacgcaaag tttttacgag aactgcaaca ccgtatgctc agagaagcca
      181 ttatcgaaca gcaaacgggc atgagcgcca tagacccatt gcgatacaaa cgggcctcat
      241 ctcctttagt tgacgacaat tccaatgcgg ataaaacacg tccaccacac tgattacatt
      301 tttcattagc acagtggtgt gatgcagcat acgaggaaaa ccggatgaat gagaacaaaa
      361 taaaaaggct ggagcaactg ttacaggcac gccaacggga gtttgccact aaatggggac
      421 gtgccgcgct gccacatgaa cgcctaacct attttccact tgaagacctc attaaaaata
      481 atggcaaaaa aatcaggtct gataagaaac acttcttaga atgcaatgaa gacaaataac
      541 aacaatctgc atattgccac caaccacaga tgtggcatcg ttatcagcgc acttgatttt
      601 ctttatatcc actgacgagt ttgccggtca ttctggtatc atgcagatat ttacggcgta
      661 caagcacact agcatgacat tgtaataagg cctgagccta acgctttaat gtgtgtcata
      721 cactcagtgg cgtaatcgtt gacaatatgg tgcatgtaat cgtaagcgtc cagcgaaatc
      781 catctggtca taatctgaag catccgacaa agtggtgtcc accaaataag tgatctgcgc
      841 ccaatatcct gagccggaac tttgacaaag gccaacgaaa aatgggagcg gatgttacag
      901 agttctcggt acaggggaaa agtggtacct gtcgccagtt ctccgatctg tttaaccggg
      961 aaattatctc ctacagcttg tcagaaaggt cggtgatgga gcatcgttaa tacgatgcta
     1021 tacgatgcat tcacagtgcc cggccagagg atgccccgtc gctgcatatg gatcagggtt
     1081 ggcaatatcg aattgcaggc tataggcaaa gttaaagccc catggagtag cacaaaatat
     1141 gccgcacaaa ggaaacggtc tgaataacgc agtgatgaag aacttcttca gcacactgct
     1201 aaaacgcata gtgatcgagc gctgattctg gcgaacaact gaactaatac atcagaatct
     1261 gcattatgtt aaataaatat aaaaagatgg tttaaatacc ccgttacttt tgacttacac
     1321 tatacggtat cgcatcgttt aatattcgca ccggccagat ttttatttct attagttgtc
     1381 acaatactga atgcgtacga ccacagtatt ctggctcctg tgtggttatg ctttaattct
     1441 gcgttccggg cagataagca gttgcttgca ggaatccttc ttgtgttaat gtcagttccc
     1501 cttttaccag tgctgatttc cacattccgt ccaacagagc ttatagcctt tccctggatt
     1561 atagcattgt ccggctgaag ttctttttga ataataatag aagcactgct ggcagatcca
     1621 gtccgttttt cataacccac tgtactgata accataatct aatcagtaga aattgagtcg
     1681 aaaataagca ctactccata caggataatt agaggtcagt ttgattattc acaattcatc
     1741 atcagcattt tctatttctg acgaaatcaa tatgaaaata accatatatg ataattatta
     1801 taataacggc tttaattgga ataca
//
LOCUS       DAZKIP010000299         1798 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZKIP010000299 DAZKIP010000000
VERSION     DAZKIP010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.303"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..482
                     /locus_tag="ACHOUK_004972"
     CDS             <1..482
                     /locus_tag="ACHOUK_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP9900213.1"
                     /translation="DTAPEAVYARLHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAI
                     VGSFFTAGASMALWGSALAAGGFSATTMLFSLGASMILGGVAQMLAPKAKTPDYRATD
                     NGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGGGTVVVVGRQG"
     gene            825..>1798
                     /locus_tag="ACHOUK_004973"
     CDS             825..>1798
                     /locus_tag="ACHOUK_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9900214.1"
                     /translation="MGKGGGKAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETGLGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLLETTSKGDRNHSSVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPERPFNIRMVRETADSTSDQLQNKTLWSSYTEIIDVKQCYPNTAIV
                     GLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYAIAQYCDQTVPDGFGGTEPRMT"
BASE COUNT          432 a          443 c          564 g          359 t
ORIGIN      
        1 atgacacggc accggaggcg gtgtacgccc gtcttcacga acagctgggt gagggaacgg
       61 tcatccatat tgtgccgcga ctggccgggg ccggaaaggg tggactgcag attgtgctgg
      121 gggcggcagc catcgtgggc tctttcttca ctgccggggc atcaatggcg ttatggggtt
      181 cagccctggc agccggtggt ttttctgcca ccacgatgct gttttcactt ggagccagca
      241 tgattctggg cggtgtggcc cagatgctgg ccccgaaggc aaaaacaccg gattaccgcg
      301 caacggataa cggcagacag aacacgtact tttcctcgct ggataacatg attgcccagg
      361 ggaacccgat gccggtgcct tacggggaaa tgctggttgg ctcccgccgt atatcccagg
      421 acatcagcac ccgtgatgaa ggcgggggcg gaacggtcgt ggttgtcggg cgacagggat
      481 aaaacataaa aaaatcccgc agtgatcgcg gagctgcggg gacagacaaa tgaagatcaa
      541 tgttaaggag ttgtttttgt tactcgggca aaaaaacact aacgcagcga aattataagc
      601 gccacagtca gtgtgtgaaa atgtgaagat attcagaatt tttatgccat taccggtttt
      661 aaccaacagg attatcggtg ggcatgaaag aaaaccccgg tatctgctga taccggggtt
      721 tctctttagc atggcagaaa tgtgtttcat gcttttcggg cgaaggatat ccgacttctg
      781 tacggaatgg caagtggcgg ttaatttatt caggggaagg ctgtatggga aaaggtggcg
      841 gtaaggcaca cacgcctcgt gaggcgaagg ataatctcaa atccacgcag atgatgagtg
      901 tgattgatgc gattggtgag ggaccgatag aaggtccggt gaagggactg cagagtattc
      961 tggtgaacaa aaccccactg acggacacgg acggcaatcc cgtgatacac ggtgtgaccg
     1021 cggtctggcg tgccggggag caggagcaga caccaccgga aggctttgag tcctccggag
     1081 ctgaaaccgg actgggcgtg gaagtgacga aggcaaaacc ggtgacgcgc accattacgt
     1141 ccgcgaacat tgaccgcctg cgggtcacct tcggggtgca gtcactgttg gagaccacct
     1201 caaagggcga ccgtaatcac tcttctgtcc gactgctgat tcagttgcag cgtaacggta
     1261 actgggtgac ggaaaaggat gtcaccatta acggcaagac cacctcgcag tttctggcgt
     1321 cggtgattct ggataatctg cctgagcggc cctttaacat ccggatggtc cgggagacag
     1381 cggacagcac ctcggaccag ctgcagaata agacgctctg gtcgtcatac accgaaatca
     1441 tcgatgtgaa acagtgctac ccgaacacgg cgattgtggg gctgcaggtg gatgcggagc
     1501 agtttggcgg tcagcagatg acggtgaact accatatccg cggtcgcatc atccaggtac
     1561 cgtcaaacta tgacccggaa aaacgcacgt acagcggcat ctgggacggc agcctgaaac
     1621 cggcatacag caacaaccct gcctggtgcc tgtgggacat gctgacccac ccgcgctacg
     1681 gaatgggaaa acgcctgggg gcggcggatg tggacaagtg ggcgctgtat gccattgcgc
     1741 agtactgcga ccagacggtc ccggatggtt tcgggggcac agagccgcgg atgacttt
//
LOCUS       DAZKIP010000300         1792 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZKIP010000300 DAZKIP010000000
VERSION     DAZKIP010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.304"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(42..>1792)
                     /locus_tag="ACHOUK_004974"
     CDS             complement(42..>1792)
                     /locus_tag="ACHOUK_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP9900215.1"
                     /translation="HRETARSFGGAGSTAGYEQVTAYTLTGQLQSRHLNLPQLDCDYD
                     WNDNGQLIRISGPQESREYRYSDTGRLTGVHTTAANLDIDIPYATDPAGNRLPDPELH
                     PDSTLTAWPDNRIAEDAHYVYRYDEYGRLTEKTDRIPEGVIRMHDERTHHYHYDNQHR
                     LVFYTRIQYGEPLVESRYLYDPLGRRTGKRVWRRERDLTGWMSLSRKPEVTWYGWDGD
                     RLTTVQTGTTRIQTVYRPGSFTPLIRIETENGEREKAQRRSLAEKLQQEGSEDGHGVV
                     FPAELVRMLDRLEEEIRADRVSSESRAWLAQCGLTVEQLEKQVEPEYTPARTLHLYHC
                     DHRGLPLALISEDGNTAWSAEYDEWGNQLNEENPHHLHQPYRLPGQQHDEESGLYYNR
                     HRYYDPLQGRYITPDPIGLRGGWNMYQYPLNPIQVIDPMGLDAIENMTSGGLIYAVSG
                     VPGLIVANSITNSAYQFGYDMDAIVGGAHNGAADAMRYCYLMCRMTKTFGSTIADVIG
                     KNHEAAGDRQGQPAKERIMDLKNNTVGIACGDFSAKCSDACIEKYNIGQLFGLDGIKA
                     DNPIKAKQGSSDASNY"
BASE COUNT          332 a          560 c          442 g          458 t
ORIGIN      
        1 caaataaaca aagggtaatt aaaaaaatga caagtttgcc tctaataatt tgaagcatct
       61 gaactccctt gctttgcttt tattggatta tctgctttta taccatctaa cccgaagagt
      121 tgcccaatgt tatatttttc aatacatgca tcgctacatt tggcagaaaa atcgccacaa
      181 gcaataccga cagtgttatt tttaagatcc atgattcttt ctttagctgg ctgaccttgt
      241 ctatccccag ccgcctcatg atttttacct atcacgtcag ctattgttga tccaaatgtc
      301 ttagtcattc gacacatcaa gtaacaatat ctcattgcat cggcggcccc attatgagct
      361 ccgccaacaa tagcatccat atcataaccg aactggtaag cactgttagt aatgctgttt
      421 gcaacaatca atccaggtac accagatacg gcataaatta gtccacctga tgtcatattc
      481 tcaatcgcat ctaaccccat tgggtctatc acttgtatgg gattcaacgg atactgatac
      541 atattccatc cacctctcaa cccaatcggg tccggggtga tataccgccc ctgcaacgga
      601 tcgtagtacc ggtgacggtt atagtacagc cccgactcct catcatgctg ctggcccggc
      661 agacggtacg gctggtgcag gtgatgcggg ttctcctcat taagctggtt gccccattca
      721 tcatattccg cgctccacgc cgtattgccg tcttcgctga taagcgccag cggcagtccc
      781 cggtggtcac agtggtacag atgcagcgtg cgcgccggcg tgtattccgg ctccacctgt
      841 ttttccagtt gctccaccgt cagtccgcac tgcgcaagcc acgcccggct ttcactgctc
      901 acgcggtctg cccggatttc ttcctccagc ctgtccagca tcctcaccag ttctgccggg
      961 aacaccacac cgtgaccgtc ctcactccct tcctgctgga gtttctccgc caggctgcgg
     1021 cgctgcgctt tctcccgctc gccgttctcc gtttcgatgc ggatgagcgg tgtgaagctc
     1081 cccggccggt ataccgtctg gatacgtgtg gtgccggtct gtaccgtcgt cagcctgtcg
     1141 ccgtcccacc cgtaccaggt cacctccggt ttacgcgaca gcgacatcca ccccgtcagg
     1201 tcacgctccc gccgccacac ccgtttcccc gtccggcggc ccagcgggtc atagaggtag
     1261 cggctctcga ccagcggctc gccgtattgt atccgcgtgt agaacaccag gcggtgctgg
     1321 ttgtcgtaat ggtagtggtg ggtccgctcg tcgtgcatcc ggataacccc ttccggaatg
     1381 cggtccgtct tctccgtcag cctgccgtat tcatcgtagc ggtagacata gtgcgcatct
     1441 tccgcgatgc ggttatccgg ccacgccgtg agcgtgctgt ccggatgcag ttccgggtcc
     1501 ggcagccggt ttcctgccgg gtccgttgca tacgggatat cgatatccag gttcgctgcc
     1561 gtggtgtgga cgcccgtcag ccttcccgtg tcgctgtaac ggtactcccg gctctcctgc
     1621 gggccgctga tgcggattag ctgtccgttg tcgttccagt cgtagtcaca gtcaagctgc
     1681 gggaggttca ggtgcctgct ctgtagctgc cctgtgaggg tgtacgccgt gacctgttcg
     1741 taccccgctg tactgcccgc cccgccgaag ctgcgggccg tctcacggtg ca
//
LOCUS       DAZKIP010000301         1778 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZKIP010000301 DAZKIP010000000
VERSION     DAZKIP010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.305"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..295
                     /locus_tag="ACHOUK_004975"
     CDS             <1..295
                     /locus_tag="ACHOUK_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900216.1"
                     /translation="IKDAADEIKGSMIFNDVDGKQKATCTIRVLILKTFHDGRYRFVI
                     ESVIYDLINNYSGFILTGRLFWQGEGFGHELFPVTNKYNAWRWKNKKIIDISW"
     gene            464..>1778
                     /locus_tag="ACHOUK_004976"
     CDS             464..>1778
                     /locus_tag="ACHOUK_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase toxin
                     domain-containing protein"
                     /protein_id="HFP9900217.1"
                     /translation="MTASGTGLGYGEGDESYGYDSCGYLKAQSAGGHRISEETDQYAG
                     GHRLKQAGNTQYDYDAAGRMVSRTRHRDGYRPETERFRWDSRDQLTGYCSAQGEQWEY
                     RHDASGRRTEKRCDRKKIRFTYLWDGDSIAEIREYRDDELYSVRHLVFNGFELISQQC
                     SRVRQPHPSVAPQWVTRTNHAVNDLTGRPLMLFNSEGKTVWRPGQTSLWGLALSLPAD
                     TDYPDPRGERDAEADPGLLYAGQWQDAESGLCYNRFRYYEPETGMYLVSDPLGLQGGE
                     QTYRYVPNPCGWVDPLGLAASSKISSLMDYIGDGRRVSGHTGFLDGVRLSRSQINNIA
                     KEMEKLGIKVIRKADKYLPPNARAAFDYGLRNIYLRKNATLYEVYHEVIHAKQFAKIG
                     REAYEALGRLSREEHVLNEILKSKNLFNEAEIAHAIKYVEGLREKF"
BASE COUNT          504 a          369 c          525 g          380 t
ORIGIN      
        1 aataaaagat gctgctgatg aaataaaggg tagtatgatt tttaatgatg tagatggtaa
       61 acaaaaagca acttgcacta tcagggtgtt aatactaaaa acatttcacg atggtcgtta
      121 tagatttgta attgaatctg tgatttatga tttaattaat aattacagtg gatttatttt
      181 aacggggaga ttgttttggc aaggggaagg ttttggccat gaattattcc ccgtgacaaa
      241 caaatataat gcttggagat ggaaaaataa aaaaataata gatatttctt ggtaaggaaa
      301 atattccccg aaagaggcca catggggaat actgctcccc tcactgatac gggagtaccg
      361 ttacgacagc gcgggtaacg tatcaggcgt caccagccgg gaagattacg gacgggaaac
      421 acagcgggag taccggcttg accggaacgg ccaggtcacg gcggtgacag cctcaggcac
      481 cgggctgggc tatggcgaag gcgatgagtc ctatggctat gacagctgtg gctacctgaa
      541 ggcgcagtct gcgggcgggc accggataag tgaagagact gaccagtatg ccggaggcca
      601 ccggctgaaa caggccggaa acacgcagta tgactatgac gccgcaggcc ggatggtcag
      661 ccgcaccaga caccgtgacg gctatcgccc ggaaacagag cggttccggt gggacagccg
      721 ggaccagctg accgggtatt gcagcgcaca gggtgagcag tgggaatacc gccacgacgc
      781 cagcggcaga cgaacagaga aacgctgcga ccggaagaaa atccgcttca cgtacctgtg
      841 ggacggcgac agtattgcgg aaatccggga ataccgcgat gatgaactgt acagcgtacg
      901 gcacctggtg tttaacggct ttgagctgat aagccagcag tgcagccggg tacggcagcc
      961 gcacccgtcc gtggccccgc agtgggtgac acgaacgaat catgcggtga acgacctgac
     1021 gggccgtccg ctgatgctct ttaacagtga aggtaaaacc gtctggcggc cgggacagac
     1081 cagcctgtgg gggctggcac tcagcctgcc cgcagacaca gactacccgg acccgcgcgg
     1141 ggagcgggac gcggaagccg accccggcct gctgtatgca ggacagtggc aggatgcaga
     1201 atcggggttg tgctataacc ggttccgata ttacgagccg gaaaccggga tgtacctggt
     1261 gagtgatcca ctggggttgc agggagggga gcagacttac cggtatgtgc cgaatccttg
     1321 tgggtgggtt gatccgctgg gattggctgc aagttctaaa atcagcagtt tgatggacta
     1381 tattggcgat ggtcgtcgtg ttagtgggca tacgggtttc ctggatgggg ttcgtttatc
     1441 acgtagtcaa ataaacaata ttgctaaaga aatggagaag ctaggaatta aagtaataag
     1501 gaaagcagat aaatatttgc caccaaatgc tagggcagct tttgattatg gtcttcgcaa
     1561 tatttatctt aggaaaaatg ctaccttata tgaggtgtat catgaagtga ttcatgctaa
     1621 gcaatttgcg aaaattggac gagaagcata cgaagcacta ggacgtttat ctagggagga
     1681 acacgttcta aatgaaatat taaaaagtaa aaatttattc aatgaagcgg aaatagctca
     1741 tgccataaaa tatgttgagg gattgagaga aaaattca
//
LOCUS       DAZKIP010000302         1754 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZKIP010000302 DAZKIP010000000
VERSION     DAZKIP010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.306"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(181..1464)
                     /gene="gltA"
                     /locus_tag="ACHOUK_004977"
     CDS             complement(181..1464)
                     /gene="gltA"
                     /locus_tag="ACHOUK_004977"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFP9900218.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDVKH"
     gene            complement(1670..1753)
                     /locus_tag="ACHOUK_004978"
     CDS             complement(1670..1753)
                     /locus_tag="ACHOUK_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900219.1"
                     /translation="MSPKINLCNSVEVLTNSFGTVISGRQV"
BASE COUNT          468 a          396 c          434 g          456 t
ORIGIN      
        1 aacggtggtg gtgaatgaaa aattaggatt ttatttagtt atcggatatg tattcttgta
       61 acgtgtaatt atgtatttgt gtccgattac gtttttttgt cgatagacat aactaacgaa
      121 caaaaaaaag cccgccagat attcaggcgg gctaaacgat atatggttca aatcagataa
      181 ttaatgttta acatctgatt taaagtctcg tttttcatag ccggtgtaca actggcgcgg
      241 acgggcgatc ttcatgccgt cactgtgcat ttcgctccag tgagcgatcc agccaacggt
      301 acgggccata gcgaagatga cggtgaacat ggaagacgga atacccatcg ctttcaggat
      361 gataccagag tagaaatcga cgttcgggta cagtttcttc tcgataaagt acgggtcgtt
      421 cagcgcgatg ttttccagct ccatagccac ttccagcagg tcatctttgg tgcccagctc
      481 tttcagaact tcatggcagg tttcacgcat tacggtggcg cgcgggtcgt aatttttgta
      541 cacgcggtga ccgaagccca tcaggcggaa agaatcattt ttatctttcg cacgacgaac
      601 aaattccgga atgtgtttaa cggagctaat ttcttccagc attttcagcg ccgcttcgtt
      661 agcaccaccg tgtgcaggcc cccacagtga agcaatacct gctgcgatac aggcaaacgg
      721 gttcgcaccc gaagagccag cggtacgcac ggtggaggta gaggcgttct gttcatggtc
      781 agcgtgcagg atcagaatac ggtccatagc acgttccaga atcggattaa cttcatacgg
      841 ttcgcacggc gtggagaaca tcatattcag gaagttaccg gcataggaga gatcgttgcg
      901 cggataaaca aatggctgac caatggaata cttgtaacac atcgctgcca tggtcggcat
      961 tttcgacagc aggcggaacg cggcaatttc acgatgacga ggattgttaa catccagcga
     1021 gtcgtgatag aacgccgcca gcgcgccggt aataccacac atgactgcca ttggatgtga
     1081 gtcgcgacgg aaagcgtgga acagacgggt aatctgctcg tggatcatgg tatgacgggt
     1141 caccgtagtt ttaaattcgt catactgttc ctgagtcggt ttttcaccat tcagcaggat
     1201 gtaacaaact tccaggtagt tagaatcggt cgccagctga tcgatcggga aaccgcggtg
     1261 cagcaaaata ccttcatcac catcaataaa agtaatttta gattcgcagg atgcggttga
     1321 agtgaagcca gggtcaaagg tgaacacacc ttttgaaccg agagtacgga tatcaataac
     1381 atcttgaccc agcgtgcctt tcagcacatc cagttcaaca gctgtgtccc cgttgagggt
     1441 gagttttgct tttgtatcag ccatttaagg tctccttagc gccttattgc gtaagactgc
     1501 cggaacttaa atttgccttc gcacatcaac ctggctttac ccgtttgtta tttggctcgc
     1561 cgctctgtga aagaggggaa aacctgggta cagagctctg ggcgcttgca ggtaaaggat
     1621 ccattgatga cgaataaatg gcgaatcaag tacttagcaa tccgaattat taaacttgtc
     1681 taccactaat aactgtcccg aatgaattgg tcaatacttc cacactgtta cataagttaa
     1741 tcttaggtga aata
//
LOCUS       DAZKIP010000303         1729 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZKIP010000303 DAZKIP010000000
VERSION     DAZKIP010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.307"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..269)
                     /gene="gadE"
                     /locus_tag="ACHOUK_004979"
     CDS             complement(<1..269)
                     /gene="gadE"
                     /locus_tag="ACHOUK_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFP9900220.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFV"
     gene            complement(1068..1640)
                     /gene="hdeD"
                     /locus_tag="ACHOUK_004980"
     CDS             complement(1068..1640)
                     /gene="hdeD"
                     /locus_tag="ACHOUK_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFP9900221.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
BASE COUNT          579 a          365 c          307 g          478 t
ORIGIN      
        1 ctaacaaaat aaataggcgc tttagctttt agtttactga tgtgataagg tgccaaaaca
       61 ataattctct cggcatctaa tttctccaga aatttaatcg cttcttcatc aaggatatga
      121 ttgtgatagg tatcaatgat aaccttgaag ggtttattgc ctactttttt gatctctgac
      181 agggaattga tttttatcat ttcgtgatta tctttcaact gccaaaagcc ctgtaaaaga
      241 aaagaatctt tcgtcatgag aaaaatcata acttgctcct tagccgttat cgtttcgaac
      301 atgtcatcct tttcattatt tacatccttg tccgaatcgt tgttcaatat agtaaacgcc
      361 tataagaact ttgcccttcg tgaactaata cgtctattaa cactaatcaa tacttcagat
      421 ttatccatca cgcactccat atattgatcc taagtaagat tcctggttgt tatcagcttg
      481 taaatagtaa acaaaaagtt aacgtttgcg gattactgcc caagaataag ttatcaataa
      541 ccagtttgtg atctctgaag aatattacta aagttaaaaa gtaatccgat ttaaatatcg
      601 agtctccttg tttcgactta agctggcaat tggattgcca gctttctttt aacgcattga
      661 aattacctga gtaaataacg cattcatgtc ttcaggtttt tatatagcgt ctggagacat
      721 ggatatttat gcaatgtaat caacgccagt tgcaaaaaaa tgaacccgga ttacttattt
      781 aaccgcccat agcatcaacg cttacggaac tctttgttca ctttcccaca gcacttttaa
      841 aacattaaac ctgactacgg aaaatatcag ccatgataat gtttgaaatg gctaatttgc
      901 catggagtga aaaaaattag atgaaattca gtaggttgaa ataatcacta gcaggtaatt
      961 atttcaatga tagtgcgcaa ttgatctaca acactgcgta gcggagagag tattaatcgg
     1021 atcatagtca catcaagtga ctatgatccg ggtgacaacc gggataatta ttgctgctta
     1081 acgaacaaac tggcgaagct gaacaggctg gcggcgctaa atatcagttc aattcccacc
     1141 agtgtggaaa ccagcgttac agacaccatc ggcgttgcac caaggaatat ccaggcaatg
     1201 acgatatcca gcacaccaat aacgagctgt agccagctgc ctttcattga acgctgacga
     1261 taccaactca tcaggcgaat aacccctgca acacagaaca aaccggcaat aaatgccgca
     1321 atggcaaaaa tgcccagctc cggtgcgcgg atgaagaaat agccgatcaa taaataggcg
     1381 actgcgacga ggaaaccgga taataccggc cagaaattat gactgcggtt gctgaataat
     1441 ccaacaataa gcgcaatgcc cgaacagatt aataatgcac ctactactgt gcttaaaata
     1501 tcgccagaaa cgaacgggaa actgatacac agcaacccga cgataaacag cagcacggca
     1561 ataaactgga ttgctctgcg atgctttttc agcatctcca gatcaaactt caaaattgtt
     1621 gccttatcta tatataacat agaaccaccc tataaaatta agaagaaaat cccctgctat
     1681 caatctatgc caaaaacgcg tctaagaatg cagtcgattt aataaaaat
//
LOCUS       DAZKIP010000304         1722 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZKIP010000304 DAZKIP010000000
VERSION     DAZKIP010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.308"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..372
                     /gene="espX1"
                     /locus_tag="ACHOUK_004981"
     CDS             <1..372
                     /gene="espX1"
                     /locus_tag="ACHOUK_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HFP9900222.1"
                     /translation="NNLFIKKSNIFGTSFTNTMIKNLRCEDIMPGRWTTQLVNKHLGY
                     RYTGVFKTLASIDDKPSRFEILIPLVQTLVRDNVKLNNDVYKELKKFMHDYDKTSPEM
                     RKYLQSINESMFLMKKISHQD"
     gene            674..1207
                     /locus_tag="ACHOUK_004982"
     CDS             674..1207
                     /locus_tag="ACHOUK_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9900223.1"
                     /translation="MKKYIIPFVATTLMFSATTFAKDVDQGTLTIRGEVVDTTCYFVN
                     GDSTTDIVVEDVVTSAMNQLINNEIYTLKTGSTSHDLEIQCDGNTAPSIEVLASEFDA
                     NNFTLNKGTAENIGFAIFLDDGTLTRMRPGLKTPLKDNGSGQYFLNLNAQYARTSGNP
                     VTKGSVESTVTIKISAD"
     gene            1257..>1722
                     /locus_tag="ACHOUK_004983"
     CDS             1257..>1722
                     /locus_tag="ACHOUK_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP9900224.1"
                     /translation="MRIITALLMSFFILPVHAGVVIYGTRIIYPEKNREVLVQLMNQS
                     KNASLIQAWIDDGNTTIAPEKIQVTFILTPPVSRVAGGSGQQLKIRKMPNNLPHNKES
                     LFYLNVLDIPPNNPQNAGKNKIKLALQNRIKLLWRPSGIAPVDKKSLSQLNIK"
BASE COUNT          624 a          326 c          295 g          477 t
ORIGIN      
        1 aacaacctct tcattaaaaa aagcaacata tttggaacat cgttcactaa taccatgata
       61 aaaaatttgc gctgtgaaga tattatgcct ggccgatgga ccactcaact tgtcaataag
      121 cacttaggtt acagatacac aggagtattc aaaaccctgg caagtattga tgacaaaccg
      181 tcccgtttcg aaattttgat accattggta caaactttag ttcgcgacaa tgtaaagcta
      241 aataatgatg tgtataaaga actgaaaaag tttatgcacg attacgacaa aacatcccca
      301 gaaatgagaa aataccttca gagcatcaac gagtctatgt tccttatgaa aaaaatatct
      361 catcaagatt aaaacacccc aaaaaagaag taatttcaga atgaaattac ctgtgataat
      421 tattaattaa ctgcagagaa ataaaaaata atctgaagca tgctctgaaa catcctactc
      481 tgtcactcta atgatggcac cttgctcttc ttataaagaa gggaataatt aagaatatta
      541 aattttcaaa ctttcatcaa cttcacatca agaaattaaa aaatttaaac attaatctaa
      601 ataataacaa cctgatcaca taatcgggtt tgaaaacgag tgaccttcca taccagttaa
      661 ttaaggatga tttatgaaaa agtatattat tcccttcgtt gcgacaacgt tgatgtttag
      721 cgcaacaacc ttcgccaagg atgtcgatca aggaacacta accattagag gagaagttgt
      781 tgatacaacg tgttactttg ttaatggtga ttcgacaaca gatatcgttg tcgaagatgt
      841 tgtaaccagt gcaatgaatc aacttatcaa taatgaaatc tatactttaa aaaccggttc
      901 aacctctcat gacttagaaa ttcagtgtga cggaaataca gccccaagta tcgaagtact
      961 agcatcagag tttgatgcta ataactttac gctgaataaa ggaactgctg agaacattgg
     1021 ctttgccatt ttcctcgatg atgggacttt gaccagaatg agaccagggt taaaaacacc
     1081 gctgaaagat aatggctctg gtcaatattt tctcaattta aatgcacaat atgcccgcac
     1141 aagtggcaat cctgttacaa aaggctccgt agagtcaacc gtaacgatta aaatcagtgc
     1201 agattaacgt tttttgccgg tagctctgct accggtatta tttacggagt gattacgtga
     1261 gaataataac agcattactg atgtcatttt ttattttacc tgttcatgcc ggtgtggtca
     1321 tctatggtac gcgcattatt tatccggaaa aaaacagaga agttctcgta cagttaatga
     1381 atcaaagtaa aaatgcctct ttgattcagg catggatcga tgatgggaac accactatcg
     1441 cgccagaaaa aattcaggtc acatttattc tcacaccgcc agtaagtcgt gtagcaggtg
     1501 gttctggcca acaacttaaa ataagaaaaa tgccaaacaa tttgcctcac aataaagaaa
     1561 gtttatttta tctaaatgtt ctggatatcc ctccaaacaa tccacaaaat gcagggaaaa
     1621 acaaaattaa acttgcattg caaaaccgga taaagttact ttggcgtcca agcggaatag
     1681 ctccggtaga taaaaaaagc ctttctcaat taaatatcaa aa
//
LOCUS       DAZKIP010000305         1717 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZKIP010000305 DAZKIP010000000
VERSION     DAZKIP010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.309"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..590)
                     /gene="nleH1-2"
                     /locus_tag="ACHOUK_004984"
     CDS             complement(<1..590)
                     /gene="nleH1-2"
                     /locus_tag="ACHOUK_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector kinase
                     NleH1-2"
                     /protein_id="HFP9900225.1"
                     /translation="MLSPSSINLGCSWNSLTRNLTSPDNRVLSSVRDAAVHSDSGTQV
                     TVGNRTYRVVVTDNKFCVTRESHSGCFTNLLHRLGWPKGEISRKIEAMLNTSPVSTTI
                     ERGSVHSNRPDLPPVDYAQPELPPADYTQSELPRVSNNKSPVPGNVIGKGGNAVVYED
                     MEDTTKVLKMFTISQSHEEVTSEVRCFNQYYGSGSA"
     gene            complement(697..768)
                     /locus_tag="ACHOUK_004985"
                     /pseudo
     CDS             complement(697..768)
                     /locus_tag="ACHOUK_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
     gene            762..1717
                     /locus_tag="ACHOUK_004986"
                     /pseudo
     CDS             762..1717
                     /locus_tag="ACHOUK_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          512 a          423 c          365 g          417 t
ORIGIN      
        1 tctgcactcc cggaaccata atactgattg aaacaacgaa cttcgcttgt cacctcttca
       61 tggctttgag atatagtaaa catcttcaac acttttgttg tatcttccat atcttcatac
      121 acgacagcat taccaccttt accaataaca ttacctggca cgggtgattt attgttgcta
      181 accctcggca actctgattg agtataatcc gctggaggta actccggctg cgcataatcc
      241 actggaggta aatcaggtct gttcgaatga acagagcctc tttctatagt cgtgctcact
      301 ggcgatgtat tcagcatagc ctcaattttt ctgctaatct ctcccttagg ccatcccaac
      361 ctgtgcaaca gattagtaaa acaaccacta tgactttctc ttgtaacgca aaacttatta
      421 tcagtgacca caacacgata tgttctgttg ccaaccgtta cttgcgtccc gctatcagag
      481 tgaacagcag catcccttac agaggataaa acacgattat caggcgaagt caggtttctg
      541 gttaaagaat tccatgaaca tcccaaattt atagaagagg gcgataacat acatttcaac
      601 cttcaaaata aacctatcta attattccta acagacatcc cccatacatg acaacaaaaa
      661 ccggagccgg actccggttt ttgtgaagct atcgggttac ttcatttcgc caatattttc
      721 ccacttcccg tcagcacgca ggatttgcag cggtcttact acacactgac ccgaggacga
      781 aaaaagagtt cgggctggga agagacagaa gagtagcaat ttcagaagca ttcacaccaa
      841 cattgagctt ttcagcgaaa gtgggcacga atcattgctg gacagggtta aaggcactga
      901 ctcaatcact cttcatacgt ggcttgatcg atatgaaaca atcctcagct agagggggag
      961 caagccgaaa acattactcg actacgccag caaaatcagg gcaattcgaa gaaaattgcc
     1021 ggacaaaccg ctcactgaca tatcaacgaa agaggtggca gcaatgctaa acacctacgt
     1081 cgcagaaggt aaagcggttt ccgcaagagt aatcaggtca acccttgttg acgtttttcg
     1141 aggggcaata gccgaggggc atgtggcaac gaatccagta acaacaaccc gtgcagcaaa
     1201 gtcagaagta aggcgctcaa ggctgacagc taatgagtat gtcgctattt accatgctgc
     1261 tgagcacctc cccatctggc tgaggctgtc aatggattta gctgtcgtta cagggcagag
     1321 agtgggcgat ttgtgcagaa tgaagtggtc agacataaac gatggtcatc ttcacattgg
     1381 acagagtaaa acaggagcca aaattgccat tccgctggct ctaaccattg acgcactcga
     1441 catctcactg gttgatacac tacagaaatg cagggaggcc agcagcagtg aaacaataat
     1501 cgcatcaacc tatcacgaac cactttctcc agccacagta tcacggtatt taacaaaggc
     1561 gcgaaatgca tccgggatct cgtttgatgg agacccaccg acatttcatg aactacgtag
     1621 tctgtccgcg aggctatatc ggaaccagat tggctacaag tttgcacaac gtcttcttgg
     1681 acataaatct gattcaatgg cggcgcatta tagggac
//
LOCUS       DAZKIP010000306         1710 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZKIP010000306 DAZKIP010000000
VERSION     DAZKIP010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.310"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(215..721)
                     /gene="espH"
                     /locus_tag="ACHOUK_004987"
     CDS             complement(215..721)
                     /gene="espH"
                     /locus_tag="ACHOUK_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspH"
                     /protein_id="HFP9900226.1"
                     /translation="MSLSGAVFKTFLTSEHASWNRFNRRLHIPNEDIVDEIQLKARMQ
                     QRHHRVYPEIGDSTIVSFRGKDYAVHFIKDGPKDDYVYKVQRITPENGCFSTLFSVFS
                     GGVTKALERKLNERHITPLSSTWFPRTPLEGILAERGLSSLLRRVQSTERLDNRAIAT
                     RASSYSVL"
     gene            complement(752..1642)
                     /gene="escQ"
                     /locus_tag="ACHOUK_004988"
     CDS             complement(752..1642)
                     /gene="escQ"
                     /locus_tag="ACHOUK_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ring protein EscQ"
                     /protein_id="HFP9900227.1"
                     /translation="MKINDFYLPLLPVIGTGRLYITSKGHACHAYFREVSGHGIRFTL
                     TYSGYEGRFWISEEQFIQWCQELFPYSESRLIPEDTIKLMILWVMQTALPEGDVSVDD
                     VQFTMLNKDIYPVIENNNGENRLNVIILETTVQSLQYLINDNWQLVPHSNALFFDGYI
                     VPGWTDYPVTELRVGDSLRLYHVDDSQERRCWIVINTPLATVNLSDNNLSVTDVLAAD
                     LLSALSNETVMNRIYCAIGTVHIDIHMLRNVKKDDIINSDGYHLFGGCQLIRNNTTIA
                     YGSIVKINEDFYFTVSIVCD"
     gene            complement(1632..>1710)
                     /locus_tag="ACHOUK_004989"
     CDS             complement(1632..>1710)
                     /locus_tag="ACHOUK_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1106 domain-containing protein"
                     /protein_id="HFP9900228.1"
                     /translation="KDYVFDKLKEIFPDEAIEFTIEYEN"
BASE COUNT          535 a          365 c          287 g          523 t
ORIGIN      
        1 cagatttttt accttgataa tttctgcgct ggatgggatc atccattccg tttaacatac
       61 tcttcatcac agggacctct gatttctggc cgctaacgtc tctgaagatt cagcctgtat
      121 tatctaaaag caatgtaata tcagttaaaa agcggaaaaa ctccagaatt ataactattc
      181 tggagttaag tcaggaagaa aactcaaatg ctaatcataa tacgctataa gaggaagctc
      241 ttgttgctat agccctatta tcaagacgtt cggtagactg tacacgacgt aacaaagaag
      301 ataatcctct ttcagctaag atcccctcca ggggagtacg aggaaaccag gttgaagaaa
      361 gtggagttat atgtctctca tttaattttc tttccaaagc cttagtcaca ccgccagaaa
      421 acacgctaaa tagcgttgag aaacatccat tttccggtgt tattctttgt actttgtaca
      481 cataatcatc ttttgggcca tctttaataa agtgaacagc atagtctttg cctctaaaag
      541 agacaatggt ggaatcccct atttctggat atacccgatg atggcgttgt tgcattcggg
      601 ctttaagctg aatttcatct acgatatctt cattgggtat atgaagcctt ctgttgaacc
      661 tgttccatga cgcatgttca cttgtcagaa atgtcttgaa taccgctcct gataacgaca
      721 tgatacatct ccctatataa cgtattcctg attaatcaca tactatgcta acagtaaaat
      781 aaaaatcttc gttgattttt acaatactcc cgtatgctat cgtcgtatta ttgcgtatta
      841 actgacaacc accaaaaaga tggtatccat ctgaattaat aatgtcatct tttttaacat
      901 tacgcaacat gtgtatatca atatggactg taccgatcgc acaataaatg cgattcatga
      961 cagtctcatt gctaagagca gataacagat ccgcagccag aacatccgtg acagataaat
     1021 tattatcact cagatttact gttgctaaag gggtattaat aacaatccag caccttctct
     1081 cttgactatc atcaacatga tagagtctca gactatcacc aactctcaac tctgtaactg
     1141 gataatcagt ccacccaggt acgatatagc catcaaaaaa aagagcatta gaatgtggaa
     1201 ctaattgcca attatcatta atcagatatt gtaaactttg taccgttgtt tcgagtataa
     1261 ttacattaag tctgttttct ccattattat tttctattac cggataaata tctttattta
     1321 gcatcgtaaa ttgaacatca tcaactgaca catcaccttc cggtaaggca gtctgcatta
     1381 cccataagat catgagtttt atcgtatctt ccggtatcag ccggctttcg ctatacggga
     1441 ataactcttg gcaccactga atgaactgtt cttctgatat ccagaaacgc ccctcatagc
     1501 ccgagtatgt caacgtgaaa cgaataccat gaccgctgac ctcacgaaaa tatgcatggc
     1561 aagcatgacc tttggacgta atatagaggc gaccggttcc gataactggc aacaacggca
     1621 aataaaaatc gttaattttc atattcaatt gtgaactcaa tggcttcatc aggaaagatt
     1681 tcttttaact tatcgaaaac atagtctttt
//
LOCUS       DAZKIP010000307         1706 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZKIP010000307 DAZKIP010000000
VERSION     DAZKIP010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.311"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..637
                     /locus_tag="ACHOUK_004990"
     CDS             <1..637
                     /locus_tag="ACHOUK_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900229.1"
                     /translation="GKHSGKKSYERWVQGAKNRTVLFHYKNLITDLNQAHSCRASLGA
                     YCPFHGEILRKLDQRNEVQRVKLSIDVPFLEGLTPSELYKIRTDYESSFNAFRSVLRE
                     AAYDIEAESDPYKRQLINQHFVERIWDEGLTDIEQKIQTYKRKSNKELFLNIAPSLIG
                     FIGAPNPVSLATGAFTILKEVFSITSHSSEIKSHPSYFLLMTSKGKNKFYD"
     gene            complement(774..1292)
                     /locus_tag="ACHOUK_004991"
     CDS             complement(774..1292)
                     /locus_tag="ACHOUK_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900230.1"
                     /translation="MTILEEMEAEAWHILTTIYTYQRLMEGLTNSSTRYELANQLVAL
                     NALLEMLVIRLARLADKRKDVRSVSMFLKRGSYSASPEAVKLAAEKFLALTEPVLKIR
                     YEQIAHMKPGTLSSFEPRELPNEALRATEALIDLIDIARGHPQSYTYRVGSQEPAINL
                     RASVETSELVKI"
BASE COUNT          515 a          354 c          350 g          487 t
ORIGIN      
        1 gggaaagcat agcggaaaaa aaagttatga acggtgggtg cagggagcaa aaaatcgtac
       61 agttctcttt cattataaaa accttattac tgatcttaac caagcgcatt catgcagagc
      121 gtccctgggc gcatattgcc catttcatgg agaaattctt cgtaaactgg atcaaaggaa
      181 tgaagttcag agggttaaac tctctattga cgttccattt ctagaggggc ttactccaag
      241 tgaactctac aaaattagaa ctgactacga atcgagtttt aatgccttcc gctctgtgct
      301 acgtgaagct gcgtatgaca tagaagcaga aagtgaccca tataagaggc agctgataaa
      361 tcagcatttt gtcgagcgca tatgggatga aggattgacc gatattgagc aaaaaataca
      421 gacctataag cggaaaagta ataaagaact ttttcttaac attgcgccat cattgatagg
      481 ttttattggg gcaccaaatc cagtgtcgtt ggctacagga gcctttacga ttctaaaaga
      541 ggtttttagt ataacaagcc actcttcgga aataaagagt cacccttcat atttccttct
      601 catgacgtca aaaggcaaaa ataaatttta tgactgaagc agtaggaaat attgtgaaaa
      661 tatattcact taagcaaaaa ggttgagggg ctgccggatc tgttattaac ggggagctaa
      721 ttactttgcc gagtaaagca actcggcaaa gtagcataga cttgagtgaa ttattagatt
      781 ttaactagtt cgctagtctc tactgaagct ctcaaattaa tggcaggctc ctgtgagcca
      841 actcggtatg tgtaagactg agggtgacct ctggcaatgt caatgagatc gattagagcc
      901 tcagttgctc tcagagcttc atttggaagt tctcgaggct cgaagctact gagtgtgccg
      961 ggcttcatat gtgcaatctg ctcataccta atctttaata ctggttctgt gagtgcaagg
     1021 aacttttcag ctgccaattt cacagcctct ggtgaagcag aatacgagcc gcgtttcaaa
     1081 aacattgata cagatctcac atctttcctt ttatccgcaa gacgcgctaa acggatcacc
     1141 aacatttcca acaatgcatt gagggctacc aactgattgg caagctcata tcttgtactg
     1201 ctattagtaa ggccttccat caaccgctga tacgtataga tggttgttag gatatgccat
     1261 gcttctgctt ccatttcttc aagaatcgtc ataaatactc cacaatggtc tgatagttat
     1321 catgcagata tctaaacaac caatttaaac taaatttata attcaacacg tagcacatga
     1381 tactgaatca ctacgtataa attagatact ttaggatttt atttggacaa ccccgcccca
     1441 gaagacaatt ctaccttagt gtatataagg accgcttatg acatagagcg gactgtcaga
     1501 tgaggtttag ttctgtacta atgagccgat acaaattagc tgcatatacc tgcattccaa
     1561 ataatttcag acagatatta tcgttgtgaa tcccccgcca cctttccggt gcgcggggtt
     1621 ttgtcttttt tcaccgggaa tacatgtatg aatccgtctg atgccattga ggcaattgaa
     1681 aaaccgctct cctccctgcc ttactc
//
LOCUS       DAZKIP010000308         1693 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZKIP010000308 DAZKIP010000000
VERSION     DAZKIP010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.312"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(240..395)
                     /gene="hokD"
                     /locus_tag="ACHOUK_004992"
     CDS             complement(240..395)
                     /gene="hokD"
                     /locus_tag="ACHOUK_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFP9900231.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVF
                     TAYEPEE"
     gene            1000..>1693
                     /locus_tag="ACHOUK_004993"
     CDS             1000..>1693
                     /locus_tag="ACHOUK_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9900232.1"
                     /translation="MTKHMDIHCQTRIFNSINHDTKYKICIFTPKNIKKNNATLYILD
                     GNSANNYISDILPVIDALPNPPVLVTLGYESWNNLSIHRRAYDYTPDGENAIVDNSKP
                     AWIYFTGGGSQSFRELLLTQIMPWVSTIAPNSSRIGIWGHSLGAIFVLDCLKNNSCFN
                     YYYISAPSLLWQNERIIKIIKDDISESKHTKSICLLNGNLSLDYSASLYPEAIKAESV
                     LRNILTEKYSNFS"
BASE COUNT          504 a          356 c          343 g          490 t
ORIGIN      
        1 aaaacaccgt ccgcgcctgc tggcaaagac ggcagaatca tcagaattgt ataatttggt
       61 atcgtgcgcc atcggttgtc tctgctggcg cagcaggtgc cagttgttca ggctggcgtg
      121 cgaattgtaa accagaatgc caggaaaaaa caaaacccgc cgaagcgggt taagtgcggg
      181 tgcgttgagg atgcctgatt catcagaggt ggcgagggat ttctcccccg ccaggtctct
      241 tactcctcag gttcgtaagc tgtgaagaca gcgacctccg tctggccggt tcggactcgt
      301 acctcgcaga ggtctttcct cgttaccagt gccgtcacta tgacggttaa acagatgacg
      361 atcagggcga ttaacatcgc cttttgctgc ttcatagcct gcttctcctt gcctttcggc
      421 acgtaagagg ctaacctaga tttgccgttc atagattgag cctcagatta atgttaggcg
      481 tcttgcagga cgcgtaatgt taactggggc ttttctctat ctgccgttgg tgttcatgcc
      541 cgaggcagat agcctcaagc acccgcagct attctactta acttcccatt acctcgccaa
      601 tatgaaatcg gtcagaaaga cagacgtccc ataaggacaa caacggaaaa agagattttg
      661 cttgatgcta tttacctctg caccatgaag ttgcgccaaa atcccagtgg tgataacagg
      721 aatttggttg ttggtgattg gggaaagaag tacgttagac tgtgggttag ttcattgaca
      781 tgatagttac ctgccttcaa tttcctaagc aagcgatgta aacgataatc aatatcaaat
      841 gtattgacag gtaaaataat aacgatgtac ctttgctgat aacatatatc agtcttaaat
      901 cataatggag gtagataaaa taactaaaca cacacaacaa attttttatc agtgacatag
      961 catatcttaa gcatccttta atgctcatgg ggagaacgca tgactaaaca tatggacatt
     1021 cattgtcaaa cacgcatatt taactcaatt aaccatgaca ccaaatataa aatatgtatt
     1081 tttactccaa aaaacattaa aaaaaataat gcaactcttt atatacttga tgggaatagt
     1141 gccaacaatt acatttctga tattctgcct gttattgatg cactacccaa tccaccagtg
     1201 ttagttacac ttggttatga atcttggaat aaccttagca ttcatcgccg tgcttacgat
     1261 tatactcctg atggtgaaaa tgctattgtt gataactcta aaccagcatg gatctacttt
     1321 actggtggag gaagccagtc tttccgagaa ttattactaa ctcagatcat gccatgggtt
     1381 agtactattg ctccgaacag ttccagaatc ggtatatggg ggcactcgct aggtgctatt
     1441 tttgtgcttg attgcttaaa aaataattca tgctttaact attattatat atctgcacca
     1501 tcacttctat ggcagaatga aagaatcatc aagataatta aagatgatat atcagaatca
     1561 aagcacacta aaagtatatg cttgcttaac ggaaatctta gccttgatta ctccgcatct
     1621 ttatatcctg aagccatcaa agcggaatct gttttgagaa atattttgac agaaaaatac
     1681 agtaattttt cta
//
LOCUS       DAZKIP010000309         1691 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZKIP010000309 DAZKIP010000000
VERSION     DAZKIP010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.313"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            113..1213
                     /gene="ompC"
                     /locus_tag="ACHOUK_004994"
     CDS             113..1213
                     /gene="ompC"
                     /locus_tag="ACHOUK_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFP9900233.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRDNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGVATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGIDLGRYGDQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            1376..>1691
                     /locus_tag="ACHOUK_004995"
     CDS             1376..>1691
                     /locus_tag="ACHOUK_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001359769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFP9900234.1"
                     /translation="MIMTNINTACVKNNASYQFNNALPNKETISSNFCERLEQWGNKS
                     LNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDFT
                     DNA"
BASE COUNT          505 a          340 c          348 g          498 t
ORIGIN      
        1 tcattgtttt taatcaccat tctaatttaa aaacagtatg tcatttataa atggcgccgt
       61 ttaaaattcg tttaaaaaat acgtttaacg ataattaaaa ggattctgtt aaatgaaatt
      121 aaaaatagtt gcggtggttg taactggttt gttagctgcg aacgtagcac acgctgccga
      181 agtctataac aaggatggta ataaactcga cctttatggc aaggttaccg ctctacgtta
      241 ttttactgat gataagcgtg acgatggtga taaaacttat gcccgtctcg gctttaaagg
      301 agaaacgcaa atcaatgatc aaatgattgg ttttggtcac tgggaatatg attttaaagg
      361 ctataacgat gaagccaacg gctcgcgcga caacaagacc cgtctggcct atgctggttt
      421 aaaaattagt gaatttggct ctctggacta tggccgtaac tacggtgtcg gctatgacat
      481 tggttcatgg actgatatgt tgccagaatt tggtggcgat acctggagtc agaaagatgt
      541 cttcatgaca taccgtacca ccggtgtagc aacctatcgc aactacgatt tctttggctt
      601 aattgaaggg ctgaactttg ccgcgcaata tcaaggcaaa aatgaacgta ctgacaacag
      661 tcatctttat ggtgctgact acacgcgtgc caacggtgac ggtttcggta tctcctcaac
      721 ttatgtttat gatggctttg gtatcggagc ggtgtatacc aaatccgatc ggacaaatgc
      781 gcaggaaaga gccgctgcta atcctctcaa tgcctccggt aagaatgcag aactgtgggc
      841 tacaggtata aaatatgatg ccaacaacat ctactttgca gctaattacg ctgaaacatt
      901 aaacatgacc acctatggcg atggttatat ttctaacaaa gcacaaagtt ttgaagtggt
      961 ggcgcaatat caattcgact tcggcttgcg cccctcactc gcttacctga aatcgaaagg
     1021 catagatctg ggccgctacg gcgatcagga catgattgag tatatcgacg ttggtgcgac
     1081 gtatttcttc aacaaaaata tgtcgaccta tgttgattat aaaatcaacc tgattgatga
     1141 aagcgacttt acccgtgccg tagatattcg caccgataac atcgtcgcta cgggcattac
     1201 ctatcagttc taacatcctc tcctgaccag cccttctctg ggctggtttt tgatttaagt
     1261 gctgctgtat gactccttaa gtaaaaatga gcaatttttg atatttctgg tcattcacct
     1321 caattaatat tattccatac ccccggggac atcttactat aatatttatt gatgagtgat
     1381 tatgaccaat attaatacag cttgtgtaaa aaataatgcc agttatcaat ttaataacgc
     1441 attacctaac aaagaaacca tctccagcaa tttttgtgaa cgactagaac aatggggtaa
     1501 taagtcgctt aataatggtg aagaaagagc aattgccgtg gagcgaatta aagaagctta
     1561 caattcgaac atggcatctc ttgatttgtc atatcttgat ttgagtgagt taccgcctat
     1621 cccctcgaca gttaatacgt taaatctgga aaacaattgt ctaacttgtc ttgactttac
     1681 tgacaatgcc a
//
LOCUS       DAZKIP010000310         1614 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZKIP010000310 DAZKIP010000000
VERSION     DAZKIP010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.314"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..258
                     /locus_tag="ACHOUK_004996"
     CDS             <1..258
                     /locus_tag="ACHOUK_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP9900235.1"
                     /translation="ASFVLATPTETDGALFPGRIMLANTCMWDYRGDECGYHGPAVAD
                     EFDNPTTDIRKDRCSKCMRGCEMRGMVANFGGFLSINKLSQ"
     gene            complement(275..529)
                     /locus_tag="ACHOUK_004997"
     CDS             complement(275..529)
                     /locus_tag="ACHOUK_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFP9900236.1"
                     /translation="MTKVRDIAPYSVRMPDSLKRDLTIRASKNGRSLNSEIVMILQAA
                     IDEEKSPRSIEGFAQQESEKFREALLKTLSSMYGEDKKPT"
     gene            639..749
                     /locus_tag="ACHOUK_004998"
     CDS             639..749
                     /locus_tag="ACHOUK_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFP9900237.1"
                     /translation="MEKEVSRILVRIPQSLKDAITGKAKEECRSFNSEVM"
     gene            1052..>1614
                     /locus_tag="ACHOUK_004999"
     CDS             1052..>1614
                     /locus_tag="ACHOUK_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFP9900238.1"
                     /translation="MKSIAKAQNDFTIFKFGDSEIRVINKCGEPWFVAKDVCDALALT
                     NSRKALTALDDDEKGVTLSYTLGGEQNLSIVSESGMYTLVLRCRDAVNKGSVPHKFRK
                     WVTAEVLPSIRKHGEYVKGKKTTVEERTPLRDAVNMLVGKKGLRNDDAYNMVHQRFGI
                     DSIDELSIEQIPLAVEYIHRVVLEGEF"
BASE COUNT          461 a          313 c          404 g          436 t
ORIGIN      
        1 gcctcgtttg tgctggcaac accgacggag acggacggag cgctgtttcc cggtcgcatc
       61 atgctggcga acacctgtat gtgggattac cggggagatg aatgcggtta tcacggtcct
      121 gcggtggcgg atgagttcga caaccccacc acggatatcc gtaaggacag atgcagcaag
      181 tgcatgcgcg ggtgtgagat gcgcggcatg gtggctaatt ttggcggttt cctttccatt
      241 aataaacttt cgcagtaatg gattatgccc accatcaggt gggtttttta tcttcgccgt
      301 acatgctgct gagagtctta agaagagcct ccctaaattt ttcagattct tgttgagcaa
      361 aaccttctat tgatcttggt gatttttctt catcaatagc agcttgcaag atcataacta
      421 tttctgaatt taatgagcgt ccgtttttcg atgctcttat ggtcaggtcg cgcttgaggc
      481 tatctggcat tcttacgcta tatggggcta tgtctctaac cttcgtcata cttacaccgt
      541 gataatcaca ttgatatcac agtgtattca aaaaaacttt gacttgatat agtcacagtg
      601 atacgtttgt gattcataat aactcacgcg gtggcgatat ggaaaaagaa gttagcagaa
      661 ttttggtgag aatacctcag tcgctaaaag atgcgattac aggaaaggcc aaagaagaat
      721 gccggtcgtt taactcggaa gtgatgtgat tgctggacag cctgaagaga gaggggataa
      781 cggtatgagt aaagaatgtt gttttttgcg gcattagcga atcagacgct gatcaaacat
      841 acatttactc taaagaaaca ggtcggatgc tgtgtagtga ctgcgtgttt gacatcataa
      901 gatacaagta tcttggatgt tctgccagca ttagcaatat aggtgaagtg tacgaaggga
      961 aagatataac tgatagagca gaaagttgaa gccccaactg ctgtaacagt cagggcttcg
     1021 ttatcaacaa atcggcttag gaaatattga catgaaaagt atagcaaagg cacaaaacga
     1081 tttcactatc ttcaaattcg gcgacagtga aatccgcgtc atcaacaagt gcggtgagcc
     1141 gtggtttgta gctaaagatg tttgtgatgc tttagctttg actaactcac gcaaggcgct
     1201 tactgcactt gatgacgatg aaaagggagt aactttaagt tacacccttg gtggtgagca
     1261 gaatctaagc attgtgagcg aatcaggtat gtatacattg gttctgcgct gccgcgatgc
     1321 agtcaataaa ggttcagtcc cgcacaaatt ccgcaagtgg gtaacagcag aagttctgcc
     1381 ttcaattcgc aaacatggcg agtatgtaaa aggaaagaaa accactgttg aggaaagaac
     1441 accgctacgc gatgcagtaa acatgctggt aggaaagaaa ggacttcgca atgacgatgc
     1501 atacaatatg gttcatcagc gttttggtat tgacagcatt gatgagcttt caattgaaca
     1561 aatcccgctt gccgtagagt acatccacag ggtagtgctt gaaggtgaat tcat
//
LOCUS       DAZKIP010000311         1562 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZKIP010000311 DAZKIP010000000
VERSION     DAZKIP010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.315"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(366..>1562)
                     /gene="nleL"
                     /locus_tag="ACHOUK_005000"
     CDS             complement(366..>1562)
                     /gene="nleL"
                     /locus_tag="ACHOUK_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector E3 ubiquitin
                     ligase NleL"
                     /protein_id="HFP9900239.1"
                     /translation="ELIKKISLDELAAFRPYVKMSLADSFSIHPYLNNANIQQWLEPI
                     CDDFFDTIMSWFNNSIMMYMENGSLLQAGMYFERHPGAMVSYNSSFIQIVMNGSRRDG
                     MQERFRELYEVYLKNEKVYPVTQQSDFGLCDGSGKPDWDDDSDLAYNWVLLSSQDDGM
                     AMMCSLSHMVDMLSPNTSTNWMSFFLYKDGEVQNTFGYSLSNLFSESFPIFSIPYHKA
                     FSQNFVSGILDILISDNELKERFIEALNSNKSDYKMIADDQQRKLACVWNPFLDGWEL
                     NAQHVDMIMGSHVLKDMPLRKQAEILFCLGGVFCKYSSSDMFGTEYDSPEILRRYANG
                     LIEQAYKTDPQVFGSVYYYNDILDRLQGRNNVFTCTAVLTDMLTEHAKESFPEIFSLY
                     YPVAWR"
BASE COUNT          566 a          336 c          211 g          449 t
ORIGIN      
        1 acaaaatcaa ttatcacatt caaaaaaata ctgacaacaa agatactctt cagacaaagt
       61 tgtcatatgt aaattttccc ttaaaacgtt gctcaagaaa aatataaaaa tcatatgccc
      121 taatcaacat cctcaagcac tacgatatat gcagtatcct attcatatat taccaccata
      181 tcagacaaat cgaatcacaa cgagttctat cgtactcttt catcttcgac actgtaaatt
      241 ttaagaacat tcggctcatg ccactaaacc aagacataat aacaacggag caataaatgt
      301 tcccactgaa catgtgcaaa cctaaaaaga cagtgttaca ataatattca tccatggtct
      361 ctaaatcaac gccacgcaac aggataatac aatgaaaata tttcaggaaa agattctttt
      421 gcatgctccg ttagcatatc agtcagcaca gcggtacaag taaaaacatt atttcttcct
      481 tgtagcctgt ctaaaatatc attgtaataa taaactgagc caaatacctg aggatctgtt
      541 ttataagctt gttcaatcaa tccatttgca tatctccgta gaatctcagg agaatcatac
      601 tctgtaccaa acatatcact cgatgagtat ttacagaaaa ccccccctaa acaaaataat
      661 atttcagcct gttttcttag tggcatatct ttcaatacat ggctccccat aatcatatct
      721 acatgctgag cgttcagttc ccaaccatca agaaagggat tccagacaca ggcaagtttc
      781 ctttgctgat catcagcaat cattttataa tctgatttat tggaattaag tgcctcaata
      841 aatctctctt tgagttcatt atcagaaatg agtatatcca gaataccaga aacgaaattc
      901 tgggaaaaag ctttatgata aggaatactg aaaattggaa atgattcaga aaaaagattg
      961 ctcaatgaat acccaaatgt attttgaact tctccatcct tatataaaaa aaaggacatc
     1021 caattagttg atgtattagg agataacata tcaaccatat gactcaaaga acacatcatt
     1081 gccataccat catcctgtga tgataacaaa acccagttat aagccaaatc ggaatcatca
     1141 tcccagtcag gcttcccaga gccatcgcac aatccaaaat cactctgctg tgtgacagga
     1201 taaacttttt cattttttaa atatacttca tagagttccc taaatcgttc ctgcattcca
     1261 tcacgccgtg aaccattcat tacaatttgt ataaaggaac tattataaga taccatcgca
     1321 cctggatgtc gctcaaaata catccctgcc tgcaataaac taccattctc catatacatc
     1381 attattgaat tattaaacca agacattata gtatcaaaaa agtcatcaca tataggctcg
     1441 agccattgct gtatatttgc gttgttcaaa taaggatgaa tactaaatga atcagccaaa
     1501 gacatcttaa catagggccg gaacgccgct aattcgtcaa gagatatttt tttgataagt
     1561 tc
//
LOCUS       DAZKIP010000312         1554 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZKIP010000312 DAZKIP010000000
VERSION     DAZKIP010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.316"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..258
                     /locus_tag="ACHOUK_005001"
     CDS             <1..258
                     /locus_tag="ACHOUK_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP9900240.1"
                     /translation="ASFVLATPTETDGALFPGRIMLANTCMWDYRGDECGYNGPAVAD
                     EFDNPTTDIRKDRCSKCMRGCELRRNVGNFGGFLSINKLSQ"
     gene            complement(275..595)
                     /locus_tag="ACHOUK_005002"
     CDS             complement(275..595)
                     /locus_tag="ACHOUK_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFP9900241.1"
                     /translation="MAEKQVKDYDKFNLRFPDGMRDAIAERAKRNGRSMNSEIVQILE
                     DALNAENTLGEIADKINSVSVPLNVDALVQLQAQVIAMQKEIQEKFREQNEKLRELLN
                     KKPT"
     gene            703..876
                     /locus_tag="ACHOUK_005003"
     CDS             703..876
                     /locus_tag="ACHOUK_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFP9900242.1"
                     /translation="MQDTLFTERKNIKLNLRLPSRLNEDLRRLAEMDCISLNSAIVRL
                     LAKGVREEVANGR"
     gene            947..>1554
                     /locus_tag="ACHOUK_005004"
     CDS             947..>1554
                     /locus_tag="ACHOUK_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001428824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFP9900243.1"
                     /translation="MTSLAIADRTINVPFHGTNLFLVGINNEPYVPMKPVVEGMGMVW
                     AAQFVKLKQRFVKGISEIEIPSAGGKQLMTCLAFRKFAAWLSSIQPNKVRPEIRDKVI
                     QYQEECDDVLYEYWTKGHVINPRKAKKALPGKITTEQQEAIKQLVMSRGQSLPKEKQA
                     KAMITMWSSLKSHFGCSYKEISEEQFTEALSLAARVPLEGEL"
BASE COUNT          408 a          344 c          372 g          430 t
ORIGIN      
        1 gcctcgtttg tgctggccac accgaccgag acggacgggg cgctgtttcc cggtcgcatc
       61 atgctggcga acacctgtat gtgggattac cggggagatg aatgcgggta taacggtcct
      121 gcggtggcgg atgagttcga caaccccacc acggatatcc gtaaggacag atgcagcaag
      181 tgcatgcgcg ggtgtgaact gcgcaggaat gtcggcaatt ttggcggttt cctttccatt
      241 aataaacttt cgcagtaatg gattatgccc accgtcaggt gggtttttta tttagtagtt
      301 ctctcaactt ttcgttctgc tctctgaact tttcctgtat ttctttttgc atggcgataa
      361 cctgggcttg aagttgaact agcgcatcaa catttagcgg aaccgagacg ctgttaattt
      421 tatctgctat ttccccgagt gtattttctg cattcaaggc atcttccagt atctgaacaa
      481 tctctgagtt catagagcgc ccgtttcgtt tggctcgttc agctatagca tctcgcattc
      541 cgtcaggaaa acggaggttg aacttgtcgt agtcttttac ttgtttttcg gccattgcaa
      601 atctctcaaa aaaaatcatg gtgccatatt gccatacgat ttcaatggtg gcattatggc
      661 cttcatggcg tcactttggc accaaataaa ggagattaga taatgcaaga tacacttttc
      721 actgagcgca aaaatatcaa actcaacctt cgccttccat cacggctgaa tgaagacctt
      781 cgccgcctgg cggaaatgga ctgtatatct ctgaactctg caattgttcg tttgctggca
      841 aaaggtgtta gggaagaggt ggcgaatggt cgctaaaaac agcgaagcct caatggctgc
      901 aaccattgag gcttctaaat taccagttaa ccacgagaaa actgatatga ctagtttagc
      961 aattgcagat cgcacaatca atgttccatt ccacggaaca aatctctttt tggttggaat
     1021 taacaatgag ccttatgttc ctatgaagcc tgttgttgaa ggtatgggga tggtttgggc
     1081 tgctcaattt gttaagttaa aacagaggtt tgtcaaaggt atttcggaaa tcgaaatacc
     1141 ttctgctggc ggtaaacagt taatgacatg tcttgccttt cgtaaatttg cggcttggct
     1201 ttcaagcatt caaccaaaca aagtccgccc tgaaatccgc gacaaggtaa tccagtatca
     1261 ggaagagtgt gacgatgtgc tctacgagta ctggactaaa ggccatgtga ttaacccgcg
     1321 caaagctaaa aaggcattgc cggggaaaat caccactgaa cagcaggaag ccattaaaca
     1381 actcgtcatg agtcgcggtc agtctctgcc aaaagaaaaa caggcgaagg cgatgatcac
     1441 catgtggtcg tcactgaaat ctcattttgg gtgttcatac aaagaaatca gcgaggagca
     1501 gtttaccgaa gctctgtcac ttgctgctcg cgttccgctt gaaggagagt taat
//
LOCUS       DAZKIP010000313         1541 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZKIP010000313 DAZKIP010000000
VERSION     DAZKIP010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.317"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(1..1129)
                     /locus_tag="ACHOUK_005005"
                     /pseudo
     CDS             complement(1..1129)
                     /locus_tag="ACHOUK_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
     gene            complement(1370..>1541)
                     /locus_tag="ACHOUK_005006"
     CDS             complement(1370..>1541)
                     /locus_tag="ACHOUK_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFP9900244.1"
                     /translation="QSPSLQKSPPTFLKPFVAGVCAALLAVSVAIPGWQFLTQPSPEE
                     QHFTWGNGCKKQ"
BASE COUNT          433 a          352 c          298 g          458 t
ORIGIN      
        1 atcaatggtt tgactttcag tatcatgaag tttaacaaca aatcaattct gtttctgaat
       61 ccaatccgcc tgtcagaaag gtaatgaata taagccatca ggtcggttgt tttttgtgag
      121 gaaaatcggt atttgtgaga aaaaagctcc tccggtcgaa gattctgact ctgccattgc
      181 tgcaggtatt tatactcaat attcagctca ttaatttctg cttttaacga agcgacctgg
      241 ttaatggcat tcaggtgttt tttcacttgt tgaagtacat catcaatcgc atttgcagac
      301 ggtgagtcag gtaagacttc ctcagaacgc gaaatcgacg ttgaaaaaaa ctgttgcctg
      361 ttctccgtac tccccagtcg tgccatcaca tatcccagtt gctgtttgtc catcttttca
      421 taaacattac tgacagctga gttattattg gacaaaattg ccactgtttt attctgaatt
      481 aatatattag caacaatatt gagtatagtc tgtgttttac ctgttccagg tggtccttca
      541 ataactgaaa tctgtgagga aaatgcccgt tcgacagcca gcaactgact ttcattaaga
      601 ccaaacggaa aaatcaaacc ccatggcgaa tcacgttttt actctgtcct gaacagtatg
      661 catgcaaggc ggtatccgga tgaggaacta ttttctttat ctgcctaagg atattctcat
      721 cgatatttct gttattacca cgggcataaa aaatacgttc attagccacc ctacacaagt
      781 aattaaacac ggcatgctct gtaaatgctg aacactgttg aaagtcaagg ccggtcgttt
      841 tcatgatgta gggtcgttca ccctcgcggt aataaacagc agcatattta tcaccatata
      901 taataaccag ttcagcccgg gatgttacag cgttcccttt cctttcgagg aaaacatttc
      961 gcaaatttac agttggcgtt atctgacata cagaaagcgg acgagtatat tttctcccgc
     1021 tgcgaaaatg acaggtcagc ataacctctc tgtcgtcatt tccagcccac agcgtccagt
     1081 cggtaatttt atgagtctta ttttcaccgt caacacaaat cgtgaccata ttcttactca
     1141 gaaattatca tttaacttac ggaaaattgt taaggcactc ccgatattag ctccggagga
     1201 ttttccacaa ctccgggact gttaacatat ttctgcctgc catatataca ttgaatttag
     1261 tcataacagt caataccaga attacaggaa tgcgatgccg atacgctggt tattgaatac
     1321 tggtagcccc tgagcactaa ttcatcacga tactctgtat ttactgtttt cactgctttt
     1381 tacaaccatt cccccaggta aaatgctgct cctccggtga tggctgtgtc agaaactgcc
     1441 atcccggaat agccacgctg accgccagca atgccgcaca gacaccagcc acaaacggtt
     1501 tcagaaacgt gggcggggat ttctgaaggg agggtgactg c
//
LOCUS       DAZKIP010000314         1538 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZKIP010000314 DAZKIP010000000
VERSION     DAZKIP010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.318"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            32..301
                     /locus_tag="ACHOUK_005007"
     CDS             32..301
                     /locus_tag="ACHOUK_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFP9900245.1"
                     /translation="MNILKKIMQRLCGCGKHDDCEHGQSLTVQLRLGPADILESDENG
                     IIPEQVRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            303..380
                     /locus_tag="ACHOUK_005008"
                     /pseudo
     CDS             303..380
                     /locus_tag="ACHOUK_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309586.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
     gene            413..985
                     /locus_tag="ACHOUK_005009"
     CDS             413..985
                     /locus_tag="ACHOUK_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFP9900246.1"
                     /translation="MPGLISYVSSASFVNEMMELRQQVMEGQIGGFLLGGERVRVSYM
                     PDTGRFLAESEGQGRVYAELLNIAFNDGVNVLRNRILSALPGMGGRNSLQEKISECAF
                     TVDIEKLQCPGDALQCPITLEQPEKGVFVKNSDGSDVCTLFDAAAFSRLTGEDLPHPL
                     TREPITASIIVKHEECIYDDTRGNFVIKGN"
     gene            1058..>1538
                     /locus_tag="ACHOUK_005010"
     CDS             1058..>1538
                     /locus_tag="ACHOUK_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFP9900247.1"
                     /translation="MPVTTLSIPSISQLSPAGVQSLQDAARLESGIRISIGSGQYSVH
                     YVQLLDGFSVEPVRGGLLDRLLGREHRMDRRAVALERQLNGGVDFLSSVNNYFQSVMA
                     EHRENKTGNKILMEKINSCVFGTDSNHFSCPESFLTCPITLDKPENGVFMRNSQGAEI
                     "
BASE COUNT          454 a          255 c          405 g          424 t
ORIGIN      
        1 ggcagtgata caggcagaac aggagaatga catgaacata ttaaaaaaaa ttatgcagcg
       61 tctgtgcggt tgcggaaagc atgatgactg tgaacacggg cagtcgctta cagtacaact
      121 gcgactgggg ccggcagaca tcctggagtc agatgagaat ggcattatcc cggagcaggt
      181 cagggtaatc acgcaggtgg tgatactgga tgcggataaa aagcagatac agtgtgtggt
      241 aagaccgctg caaatcctgc gtgctgacgg gacgtgggaa aatattggcg ggatgaagta
      301 acccgacagc ttcacaaaac cggagtccgg ctccggtttt tgttgtcatg tccggtggat
      361 gtttgttaat gaagacttga gggaatattt atccgtatga aggagcatgg taatgcctgg
      421 attaatatca tatgtgtcat cggcttcatt cgtgaatgag atgatggagc tgcgtcagca
      481 ggtaatggag gggcagattg gtggatttct cctgggaggg gagagggtta gagtttctta
      541 tatgccagat acaggccgtt ttttagcaga aagtgaaggg cagggacggg tttatgcaga
      601 attattgaat attgctttta atgatggagt taatgtgctc agaaacagga tattaagtgc
      661 gctccctgga atgggagggc gaaactcttt gcaggaaaaa atatcggagt gtgcctttac
      721 tgtcgatatt gaaaaacttc aatgtcctgg tgatgcgctt caatgtccaa ttacactgga
      781 gcagcctgaa aaaggtgttt ttgtgaagaa ttcagatggt tcagatgtat gtactttatt
      841 tgatgccgct gcattttctc gtttgactgg tgaagactta ccccacccac tgacccggga
      901 accaataacg gcatcaataa ttgtaaaaca tgaagaatgc atttatgacg ataccagagg
      961 aaacttcgtt ataaagggta attgaaatga acattaccct ttattttatt taatgaaaca
     1021 tcctgcaaac tgatatgaat tactgaatga ggtttttatg cctgttacca ccttaagtat
     1081 cccaagtata tctcaattat ctcctgcagg agtacagtct ttgcaggatg ctgccagact
     1141 tgaaagtgga ataagaatat ccattggtag tggccaatat tctgttcact atgtccaact
     1201 actggatgga ttttcagttg aaccggtgag aggaggctta ctggataggc tattggggcg
     1261 tgagcatcga atggatagaa gggctgtggc tctggaaagg caattaaatg gaggtgtcga
     1321 ttttttaagt agtgttaata actattttca gagtgtcatg gcagaacaca gagaaaataa
     1381 aacaggtaat aaaatattaa tggaaaaaat aaattcttgt gtatttggaa cggattctaa
     1441 tcacttttct tgcccggagt catttttgac atgcccgata acgctggaca aacctgagaa
     1501 tggagtgttc atgagaaact cacaaggtgc tgagatat
//
LOCUS       DAZKIP010000315         1534 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZKIP010000315 DAZKIP010000000
VERSION     DAZKIP010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.319"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..587
                     /locus_tag="ACHOUK_005011"
     CDS             <1..587
                     /locus_tag="ACHOUK_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HFP9900248.1"
                     /translation="LLILYCLGSMIIVLIFDFIAEYFLFMKDMKMDKQEVKREYKEQE
                     GNPEIKSKRRERHQEILSEQLKSDVSNSRLMIANPTHIAIGIYFKPHLSPIPLISVRE
                     TNEVALAVRKYAKEIGIPIITDKKLARKIYATHRRYDYVSFENIDEILRLLLWLEDVE
                     NAGQPVPDEQLSSEDKYIEGEDTKSENNDNNLKN"
     gene            complement(648..914)
                     /locus_tag="ACHOUK_005012"
     CDS             complement(648..914)
                     /locus_tag="ACHOUK_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HFP9900249.1"
                     /translation="MIITIALISLLSACTRENSLKENQNKRSGESQAIVDARQFFSEH
                     PEYISSAELEEKLYREFAQVTTVPAYKEMSMYQLLVISQDRLTH"
     gene            complement(944..1444)
                     /locus_tag="ACHOUK_005013"
     CDS             complement(944..1444)
                     /locus_tag="ACHOUK_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9900250.1"
                     /translation="MQVFSSDVYFTVGTNALLASQKEYYSDLVALVDLGHSFVVIDEH
                     QHRNLKPNTEPVNILLSNNFIWINKNITLSDLTHFLVSNLHTQNVYSTQEPLTHDEID
                     ILRLCVSYSLKQIAIIKGIDYKTVSYHKIRALNQLNIKGTVELFIALCEWDKHYFKLQ
                     SCVRES"
BASE COUNT          516 a          241 c          275 g          502 t
ORIGIN      
        1 tccttctcat actgtattgt ctcggcagta tgataattgt cttaattttt gattttatcg
       61 ctgaatattt tttatttatg aaagatatga aaatggataa acaagaagtt aaaagagaat
      121 acaaggaaca agaaggaaat cctgaaatta agtctaaacg cagagagcgc catcaggaaa
      181 ttctttctga gcaattgaaa tctgatgtca gtaatagccg tttgatgatt gccaacccta
      241 ctcacattgc aatagggata tactttaagc cacatctgtc acctattcca ttgatttctg
      301 taagagaaac taatgaggta gcattagctg taaggaaata tgcaaaggaa atcgggatac
      361 caattattac agataaaaaa ttagcacgaa aaatttatgc tacccatcgt cgctacgatt
      421 atgttagctt cgaaaatata gatgaaatat tacgtcttct gctgtggctt gaagatgtgg
      481 agaatgctgg acaacctgtt ccagatgaac agctctcttc agaagataaa tatattgagg
      541 gtgaagacac aaaaagcgag aataatgaca ataatttaaa aaattaaaca tgtatcgtag
      601 tcaaaaattt ataagaatct acacgcaaaa aacgtgtaga tttttattta atgagttaaa
      661 cgatcttgcg aaatgacaag caattgatac atactcatct ctttataagc tggaactgta
      721 gtaacctgtg caaattcgcg atatagcttt tcctccagtt ctgctgatga tatatattcc
      781 gggtgttcgc taaagaactg tcttgcatct actatcgctt gggattcgcc acttctttta
      841 ttttgattct cctttaatga attttctctg gtgcatgcag agagcaacga tatcagcgcg
      901 atggttatta tgattttact attatatcat tactaatgac atatcaactt tctcttacgc
      961 aagattggag tttaaaataa tgtttatccc attcacagag tgctataaac aactcaacag
     1021 taccttttat gttaagttga ttgagggctc ttattttgtg atatgatact gttttataat
     1081 caattccttt aataatggca atttgcttta atgaatatga aacacataac cgcaggatat
     1141 ctatctcatc atgggtaagt ggttcttgtg tggaatacac attttgtgta tgaagatttg
     1201 aaaccaggaa atgcgttaaa tcagataatg taatattctt attgatccag atgaaattgt
     1261 tactcaaaag gatgtttaca ggttctgtat ttggtttgag atttcgatgt tgatgctcat
     1321 caataacaac gaacgaatgt ccaagatcaa ccaacgcaac taaatcgctg taatattcct
     1381 tttgggatgc taataatgca ttagtgccta cagtgaagta gacatcactg gaaaagactt
     1441 gcatatgcca acctgtactc taaaatcatt tagggtaaat attatgtgta aaatttaaaa
     1501 taagagtaat tgttatattt gcttaacgtt gagg
//
LOCUS       DAZKIP010000316         1526 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZKIP010000316 DAZKIP010000000
VERSION     DAZKIP010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.320"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..368)
                     /locus_tag="ACHOUK_005014"
     CDS             complement(<1..368)
                     /locus_tag="ACHOUK_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP9900251.1"
                     /translation="MQDIPQETHHETTRLTQSAQAVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSSARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGN"
     gene            complement(378..707)
                     /locus_tag="ACHOUK_005015"
     CDS             complement(378..707)
                     /locus_tag="ACHOUK_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9900252.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKCEHPSLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            complement(707..>1526)
                     /locus_tag="ACHOUK_005016"
     CDS             complement(707..>1526)
                     /locus_tag="ACHOUK_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9900253.1"
                     /translation="QVQLSLRQKYQQQLEQLRRDSEQKGTYNTDDYRKAEQALTESLN
                     RQLNENRRYWQQLEVVQGNWKNGVLRAFQDFTVDADNTAETAEQVFSSAFSNMGNGLA
                     TFVTTGKLNFKSFTSSVLSDMAKILAQATMMKSIKGIGSVLGFDLSSLSLNANGGIYQ
                     SADLSRYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADIGGSGMA
                     MFSPQYNIEINNDGTNGQIGPAALKAVYDLGKKAAADFMQQQARDGGRLSGAYR"
BASE COUNT          339 a          476 c          330 g          381 t
ORIGIN      
        1 ctgtttccgt tgacgaagtt caccgcatcc agaaaacggg cgtaaacctt acgccggacc
       61 accgttccgc cgaccagact ctgcaggtct tccgccatcc ccgtgaccat gccgtgcagg
      121 ttagaaaccg tcagtgtcgg acgggcagaa ctgcccttgc cgttcagttc aaatcccgtc
      181 ccctgaatgg ggtatgcctg atactgccgc ccctgccagg taaccggctc acctttttcg
      241 ttctgctcat tacagaaaaa ataacgttca ccaccgacct ctgtcagatc gatttcccag
      301 agcaccgcct gggctgactg agtgaggcgt gtcgtctcat gatgtgtttc ctgtggaata
      361 tcctgcatca gagcctccta tgccacgacc tgttcaaaat ctgccgttat ggttacccac
      421 agcgccccca cgcttgccga ccatttacga caaaccaccc tgatcggttt ccagtcataa
      481 ggtggcgtcc actgaaatgc acggacgcca ccgtgccgtt ccagaaaggc ttttaaagat
      541 gggtgttcac atttacgaac acgtatcgtc acgctgtaag tcgacaactg gttattcagt
      601 cccgccgcac gacgctgttc ataaccatcg cccagcttca ctgtcaccac tttcggctct
      661 gataccacat tcatatccgg gcgcactttc cagtgaaacg tctccattac cgatatgctc
      721 cacttaaccg accaccatca cgggcctgct gttgcataaa gtccgctgcc gcttttttcc
      781 cgaggtcata aaccgccttc agggcagccg gacctatctg cccgttcgtg ccatcgttat
      841 tgatctcgat gttgtactgc ggggaaaaca tcgccatacc tgaaccccca atatccgcca
      901 caacccccag cttaccgtca gcaccacgac gcaatggcag aatggcttca ggtcccgctt
      961 cccccatcac acccgcgcct tttgcaaaag caaaaaacgt cggacggtta accaccgtgc
     1021 cactgtaacg actcaaatca gcagactgat aaatcccccc attggcattc agggaaaggc
     1081 tgctgagatc aaatcccagt acactgccaa tcccttttat cgatttcatc atggttgcct
     1141 gcgccaggat tttcgccata tctgacagca cagaagaggt gaaggatttg aaattgagtt
     1201 tgccggtagt gacaaaagtt gccaggccat ttcccatgtt gctgaaggct gacgagaaca
     1261 cctgttctgc tgtttctgcc gtattatctg catccacggt aaaatcctga aatgcacgca
     1321 ggactccgtt tttccagtta ccctgtacaa cttcaagctg ttgccagtaa cggcgattct
     1381 cattcagttg tcggttcagg ctctccgtca gcgcctgctc ggcctttctg tagtcatccg
     1441 tgttatatgt ccctttctgc tcactatccc gcctcaactg ctccagctgt tgctggtatt
     1501 tctggcgaag actcagttgt acctga
//
LOCUS       DAZKIP010000317         1517 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZKIP010000317 DAZKIP010000000
VERSION     DAZKIP010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.321"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..103
                     /locus_tag="ACHOUK_005017"
     CDS             <1..103
                     /locus_tag="ACHOUK_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900254.1"
                     /translation="AVKPLKFANQMYIDTGAVFCGNLTLIQVQGEGA"
     gene            100..723
                     /locus_tag="ACHOUK_005018"
     CDS             100..723
                     /locus_tag="ACHOUK_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFP9900255.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAVFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGIVAEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAITR"
     gene            976..>1517
                     /locus_tag="ACHOUK_005019"
     CDS             976..>1517
                     /locus_tag="ACHOUK_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900256.1"
                     /translation="MLSSCIGVVMKDGALLRSSSLFIAYMGCLGWGSAYFYGWGTSFY
                     YGFPWWIVGAGVDDVARSLFFAVIVIAIFLIGWGIGVVFFFAVKRKHSMQELNVFRLY
                     FAVELLFVPAIIEFSILRQKIQVPLLLLSAAIALAVTISIRSYGRFLSVSCFYDKPFI
                     KKHFFEIVMIAFVAYFWLFS"
BASE COUNT          387 a          292 c          378 g          460 t
ORIGIN      
        1 agcagtgaaa ccactcaaat ttgccaacca gatgtatatc gatactggcg cagtgttctg
       61 cggaaacctc acattgattc aggtacaggg agaaggcgca tgagactcga aagcgtagct
      121 aaatttcatt cgccaaaaag cccgatgatg agcgactcac cacgggccac ggcttctgac
      181 tctctttccg gtactgatgt gatggctgct atggggatgg cgcaatcaca agccggattc
      241 ggaatggctg tattctgtgg taagcacgaa ctcagccaga acgacaaaca aaaggctatc
      301 aactatctga tgcaatttgc acacaaggta tcggggaaat accgtggcgt ggcaaagctt
      361 gaaggaaata ctaaggcaaa ggtactgcaa gtgctcgcaa cattcgctta tgcggattat
      421 tgccgtagtg ccgctacgcc gggcgcaaga tgcagagatt gccacggtac aggccgtgcg
      481 gttgatatag ccaaaacaga gcagtggggg atagttgctg agaaagagtg cggaagatgt
      541 aaaggcgtcg gttattcaag aatgccagca agcgccgcat atcgcgctgt gacgatgcta
      601 atcccaaacc ttacccaacc cacctggtca cgcactgtta agccgctgta tgacgctctg
      661 gttgtgcaat gccacaagga agagtcaatc gcagacaaca ttttgaatgc gatcacacgt
      721 tagcgccatg attgccacgg atggcaacat attaacggca taatattgac tttttgaata
      781 actttgggga aacttgacac caataatggg cgttttttac atgtcattga tgagtctcaa
      841 taacctgccg ccgagtagtt tttatgctct gaattgtatt tgtgtagtaa acatgctgac
      901 tgcaatgtaa tagagttttt ttagcctgta acctcttgac ggcattgaat tgcttttgtt
      961 atgagttgta agccaatgtt atcatcttgt attggggtgg ttatgaagga tggtgcgctg
     1021 ctcaggagtt cttcactttt tattgcctac atgggatgcc ttggatgggg gagtgcttat
     1081 ttctatggat ggggtacttc tttttactac ggcttcccat ggtggattgt aggtgcaggt
     1141 gttgatgatg ttgccagaag tttatttttt gcagttatcg tcattgctat atttcttatc
     1201 ggttggggta ttggtgttgt attctttttc gcagtgaaaa gaaaacattc tatgcaagag
     1261 ctaaatgtat ttcgccttta ttttgctgtg gaattattgt ttgtgccggc aattattgag
     1321 ttttctatat tgagacagaa gattcaggta cctcttttgc tactgtcagc agcgattgcg
     1381 ctggcggtca caatttcgat aagatcttat gggcgatttt tatcggtatc atgcttctat
     1441 gataagccat ttataaaaaa acattttttt gagattgtga tgattgcttt tgtggcatat
     1501 ttctggcttt tttcatt
//
LOCUS       DAZKIP010000318         1448 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZKIP010000318 DAZKIP010000000
VERSION     DAZKIP010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.322"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            168..>1448
                     /gene="espK"
                     /locus_tag="ACHOUK_005020"
     CDS             168..>1448
                     /gene="espK"
                     /locus_tag="ACHOUK_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspK"
                     /protein_id="HFP9900257.1"
                     /translation="MLPTSQLRPTGTFCSYSAETSADIKSEITPIQIEEARASGRLYI
                     KDCDIEYLPQLPNEITSVTIENCNNLTTLTGLPVNTQNLSVINCEKLQITDMPSTVKN
                     LHIELTDSPFIHFISEGIECLTVCHCYISGVPESVRYLEIKGSATDSIKNVPNGLSSL
                     SINSYNPENQARIDNLISPSLKTLSLTGCSNIILPEKLPESVTSVTIHAEQKTTWNIG
                     VEGMPDGLDLDLQNVLLSPDVVKAKNITFQGNALDVALHFREGDIVYGLSSPREKLVN
                     SIKLVNDFSKKDIITQNTLTNAVWDPRTPRKYKQDPLIKRALNEHERGIKFKQHLKNH
                     NNYNVTMADLSVYNRDKLWAKTSKAGLEFQTLTRNKTVIFCADELVNSLKLIANKSEG
                     YGQSITASELRWIYRNKDNNQIMKNIKFYLHGKEI"
BASE COUNT          508 a          305 c          267 g          368 t
ORIGIN      
        1 ggagaaagta ttcgcaaaag ttaaaatcaa cagtctgttg gttttgagat ataactctct
       61 tttctaatat tgacaccttg gctttgctgg ttgttaaaat tagcttgttg attagtagta
      121 tggatagaac ttactctgtt atttatttct aaaaatggaa tttatcaatg cttcctacat
      181 cgcaattacg accgaccggg acattctgct cctattccgc tgaaacatca gcagacatca
      241 aaagcgaaat cacaccaatt cagatagaag aagcgcgggc cagtggtcgt ttatatatca
      301 aagattgtga tattgagtat ctgccacagt taccaaacga aataacatca gttacaatcg
      361 aaaactgcaa caacctgaca acccttacag gattgccggt taatacacaa aacctctccg
      421 tcattaactg tgaaaaatta caaatcacag acatgccatc aaccgtaaaa aatctacata
      481 ttgaattaac tgattcacca tttatacatt tcatatctga aggcatcgag tgcctgacgg
      541 tttgccactg ctatatatct ggagtgccag agagtgtccg ctaccttgaa ataaaaggta
      601 gcgccacaga cagcataaaa aatgttccaa acgggttatc atctctcagc atcaatagct
      661 ataacccgga gaatcaggcc agaattgaca acctgatatc accgtcactg aagacgctat
      721 cgctgactgg atgtagcaat attatactgc cggagaaact tccggagagt gtgacatcgg
      781 taaccattca tgcggagcag aaaaccacgt ggaacatcgg tgttgaaggg atgcctgatg
      841 ggctggatct tgatttacaa aatgtactac tctctccaga tgtagttaaa gcaaaaaaca
      901 tcacctttca gggcaacgct ctggatgtgg ccttacactt tcgcgaggga gacattgtct
      961 atggactatc ttcacccaga gaaaaacttg tcaacagcat taaactagtt aacgactttt
     1021 ccaaaaaaga tattataact cagaatacgt taacaaacgc agtatgggac cccagaacac
     1081 ctcgcaaata taagcaagat ccacttatca aaagagcatt aaatgaacac gaaagaggaa
     1141 taaaatttaa acaacactta aagaatcaca ataattataa tgttaccatg gccgaccttt
     1201 ccgtatacaa tcgcgacaaa ttatgggcaa aaacaagcaa ggccggccta gagtttcaga
     1261 cattaacacg caataaaacg gttatttttt gtgcggatga gcttgtcaac tcactcaaac
     1321 tcatagctaa caagtcagag ggctatggcc agagtattac cgccagcgaa ttacgatgga
     1381 tttaccgtaa taaagacaac aaccaaataa tgaaaaacat aaaattttat ctacatggca
     1441 aagagata
//
LOCUS       DAZKIP010000319         1430 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZKIP010000319 DAZKIP010000000
VERSION     DAZKIP010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.323"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            165..428
                     /locus_tag="ACHOUK_005021"
     CDS             165..428
                     /locus_tag="ACHOUK_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFP9900258.1"
                     /translation="MKPNIHPEYRTVVFHDTSIDEYFRIGSTIKTDRVIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVDAKKGG"
     gene            730..870
                     /locus_tag="ACHOUK_005022"
     CDS             730..870
                     /locus_tag="ACHOUK_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP9900259.1"
                     /translation="MPQKTIIVGMLCLTMLLTVWVLHASPCEFRVSFMWSEIAAFLQC
                     KP"
BASE COUNT          386 a          297 c          351 g          396 t
ORIGIN      
        1 cggctcgctt cttatttaat ggattttttc gggaagtcca gtttataatt atataacata
       61 ttgtaatact gaaagagagt gaggttataa gaacagacag ttgaatctga ccgtctgaat
      121 atgtaccttt atgcggaatt ttcacttaaa gggggaaaac tgttgtgaaa ccgaatatcc
      181 atccggaata ccgtacagta gtatttcacg ataccagtat tgatgagtac ttcaggatcg
      241 gttcgacgat caaaactgac cgggtaattg aactggatgg tgtaacttac ccatatgtta
      301 caattgacgt ttcttctaag tcgcatccgt tttatacagg taaactgaga actgtcgctt
      361 cagaaggtaa tgttgcacgc tttacccagc gttttgggcg ttttgttgat gcgaaaaaag
      421 gtggctgatt ttgccgccag ggtgcgtttg acaatttaaa aacgcaccag acagagagat
      481 cctgcacctg aatataccgt tagcccgtcc caccggggcg tgtgcagatg atctcgtcac
      541 cggaagattg cggacctctc tgaaaaccag tacattacgc gcgggtgcct ttcggctgac
      601 ggctggaagg attacctgaa ggccggatgt ggaaaggccc cgagtcaact taaacgttaa
      661 cccgaggccc tgaccatcta ccttcagcaa gtggaaggtt agcgcctctc tgtaaaagga
      721 gcaagtgcta tgccgcaaaa aacgatcatt gtgggcatgt tatgcctgac catgttgctg
      781 accgtctggg tgttacacgc atcaccgtgt gaattcaggg taagcttcat gtggtcggaa
      841 attgcggcgt tcttgcagtg taaaccgtaa gaaaccgcag cggggacaac agtccccgct
      901 atccggttgc caaaggttcg gtcgaaagtc gcacccttgc acacaaatca caaagaaaaa
      961 acaaaagtcc gcagtgtaaa aactgtgggc ttttttattc gcatcccgaa agtgtggtaa
     1021 cagcgtgggg gcaccaaaaa aagccccata aaatggggct gaagattctg taaggaattt
     1081 cacagcaatg agattcctgc tttctccaag gatatacgtg tcttcaggct gaatcaatgg
     1141 gggcgtggct ctgaatccac ctgcgattaa aacttgcaca atgccgggag ttgagttaga
     1201 aaagtacagg gcgcgatatc cggcttattt tgttgctgtg ttttgcctcg tgctggatgt
     1261 cgcgtccacc gtttttctga tgattcatat gaaatggtac tttattgatt tactgccatt
     1321 ctgatttgcc tttttatgtt cattattgcg gttgtcactt ccagtttctg gtgatgtttt
     1381 gataaaagtg aaagaataaa acgataattg cagaaggtat tgctaaaact
//
LOCUS       DAZKIP010000320         1400 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZKIP010000320 DAZKIP010000000
VERSION     DAZKIP010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.324"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            476..746
                     /locus_tag="ACHOUK_005023"
                     /pseudo
     CDS             476..746
                     /locus_tag="ACHOUK_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991605.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            812..1009
                     /locus_tag="ACHOUK_005024"
     CDS             812..1009
                     /locus_tag="ACHOUK_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9900260.1"
                     /translation="MLTTTSHDSVFLRADNSLIDMNYITSFTGMTDKWFYKLISEGHF
                     PKPIKLGRSSRWYKSEVEQWK"
BASE COUNT          346 a          330 c          336 g          388 t
ORIGIN      
        1 tttgcctgat ttaagccgcc gtgggtattt gtccaagaat gcttcaagct ccaatcgttt
       61 tggcctggtg aatggagatg ggttaagcaa agactctcgt tgctggtgta ctttttcgcg
      121 ctggcgctca agcatggata atgcgattgc ctgtagttct tcgttagtca ttgtctgcac
      181 ctgggaagca atttaccgga gtacctcaca ctacggcaaa aatgttttat tgttgcaact
      241 tgcgtaatta ttcaggtcag tttggaacgc actgtataaa cataaataac ctgatactgt
      301 tgtgtgtttt caaagaaaac acaggtattt ttcacataaa ccttcccctt ttcatatccg
      361 accacttaat caaaaaacgg ttttatactt gttgccccca ctggggtcat cttgggtgac
      421 ttttaacgtc aggtgacgca ttgtcacttc agacctgcta cctatacgcc ggaatataat
      481 ggtcagagag gcatatttcg acgcccctaa tctggggcgt ttgattcagg atgtctggaa
      541 caatccgggc tttgtgtcac cgggcgcatc aaagctgtac cacccgggat gtggcgctgg
      601 atcgtcattt accggactgc gaagacatac ttaaagaggt gatatgggcg ttcagtgatt
      661 ttgtccgcga taatcgtggt gtgtatgatc cggaagcccg ttaccccgcc ggtaatccct
      721 ggtatcccgt aacaggccag ttctgaaagc acagctcagt agatttcccg cattcgcaca
      781 atcttcataa atctcacagg acattctgac catgttgact acaacaagcc acgacagcgt
      841 atttctgcgt gccgataatt ccctgatcga catgaactat atcaccagtt tcaccggtat
      901 gaccgacaaa tggttttaca agttgatcag tgaaggccat ttccctaaac ccatcaaact
      961 ggggcgcagc agccgctggt acaaaagtga agtggagcag tggaagtgaa gtagagcagt
     1021 ggatgtagca acgaatcgag gaatcctggg gggcagcagc atgaaacgtg ttgtaatgcc
     1081 ggtacgctgg caatgtgcaa aatgccagcg ctggtattgt ggaacccggc cctgcccttg
     1141 gtgctggcga tattcccgtt catcttcctg ctgatacctt ccgttcagca gcgggttgtt
     1201 atcccttcct gactgattcg tcattccatt cttattgatt agaactggca ttacaccggt
     1261 gccggcgtgc tttcctgcgt gtctgcgccg gttcagtaaa ttagaaaaga aacatttgtc
     1321 agcgccagtg atataaggcg taacggctaa caatcttggc gttaatacat tccgagcaaa
     1381 taaccgaagc gttactttta
//
LOCUS       DAZKIP010000321         1383 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZKIP010000321 DAZKIP010000000
VERSION     DAZKIP010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.325"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..501)
                     /locus_tag="ACHOUK_005025"
     CDS             complement(<1..501)
                     /locus_tag="ACHOUK_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900261.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPTGNNLEALAPGIEKLKQ
                     TSIEMVTLLNTLQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNAQIIQRSD
                     NTCELTINEPLKNQEISTGNININCPLKDIYNEFRRLNVIFSCGTGDIVDLSSLDLRN
                     VDLDYYD"
     gene            complement(523..1062)
                     /gene="yhaB"
                     /locus_tag="ACHOUK_005026"
     CDS             complement(523..1062)
                     /gene="yhaB"
                     /locus_tag="ACHOUK_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
                     /protein_id="HFP9900262.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEKCTCSAVNKKELECIKETISKYCAKFTRKEAILTPPAYFNKTSIT
                     SDCWQILFFSPDHFNNDFY"
     gene            complement(1318..>1383)
                     /locus_tag="ACHOUK_005027"
     CDS             complement(1318..>1383)
                     /locus_tag="ACHOUK_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HFP9900263.1"
                     /translation="LINVNFFSSDRTSFCECNRFP"
BASE COUNT          430 a          243 c          251 g          459 t
ORIGIN      
        1 atcataataa tctaaatcga cattacgtaa gtccagagag gatagatcaa cgatatctcc
       61 agtcccacaa ctaaaaatta cgtttaacct cctgaattca ttgtaaatat cttttaatgg
      121 gcaattaata ttgatattac ctgtggatat ttcctggttt tttaacggtt cattaatggt
      181 aagttcacaa gtattgtccg atcgttgaat aatttgggcg ttataaccta aaaaatccag
      241 acgaagagac gagtcattat aaagaattac attttgtaag tacgctaatt ctttttgaaa
      301 atcaccagtg ataatgcatt ttccactagg ttgtaacgtg ttaagtaaag tgaccatttc
      361 aatagaggtc tgttttagtt tttcaatacc tggtgctagt gcttcgaggt tatttcctgt
      421 tgggctttca ggtaggtcat tcatttttcg acggactaaa tcactactaa tatcattacc
      481 aattgatgaa acagggaaca ttattttatc ctttataaat aatcaataaa aatcattatt
      541 aaaatgatcg ggtgaaaaaa agagaatttg ccaacaatct gaagtaatac tggttttatt
      601 aaagtacgca gggggtgtta agatggcttc ttttcgggtg aattttgcac aatacttaga
      661 aatcgtctcc ttaatacatt ccagctcttt tttattgacg gctgagcaag tacatttttc
      721 gccaaaaggt gcaaaagtgc ctacatctaa tgtttgtatg atataatcgt catgttctcc
      781 tgtagaaata tctatgatga atggactcat attttccggt tggattcgga tcatgacaga
      841 aggaacatcg ccttgataga aattcaatgc agtaattgca gataatactc tccgcagttc
      901 attacttata ctgtaaattt tattaacgtc attggtggag acaatttctg caaactttct
      961 ttttaccgtc caataatcaa tatttctatt gtgattgtta accactgcgt gcattggtgg
     1021 gattgaaggg tgaaaaatat gtgcaattgg gaagcctttc attgatgcct gccttgtcat
     1081 catgacaaac aataatagat gcttattttc ctcgcagggt tctttcgcac tatcaaaaaa
     1141 cgctcattta cccggcgtta cagtaaacga agcaacattc tgtggcctac gccgatcaaa
     1201 agctctgtga aaatttccct tgttgtcttt gtctgacctt atcgcggcga tcagcaatta
     1261 ttcgttttgt gatcctcaac acaaaatgct attcgtcgcc tatgaaggaa gaagaattta
     1321 tggaaaacga ttgcattcac aaaatgacgt cctgtcagaa gagaaaaagt tgacgttaat
     1381 caa
//
LOCUS       DAZKIP010000322         1382 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZKIP010000322 DAZKIP010000000
VERSION     DAZKIP010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.326"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            29..1354
                     /gene="nleA"
                     /locus_tag="ACHOUK_005028"
     CDS             29..1354
                     /gene="nleA"
                     /locus_tag="ACHOUK_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleA"
                     /protein_id="HFP9900264.1"
                     /translation="MNIQPTIQSGITSQNNQHHQTEQIPSTQIPQSELPLGCQAGFVV
                     NIPDDIQQHAPECGETTALLSLIKDKGLLSGLDEYIAPHLEEGSIGKKTLDMFGLFNV
                     TQMALEIPSSVSGISGKYGVQLNIVKPDIHPTSGNYFLQIFPLHDEIGFNFKDLPGPL
                     KNALSNSNISTTAVSTIASTGTSATTSTVTTEPKDPIPWFGLTAQVVRNHGVELPIVK
                     TENGWKLVGETPLTPDGPKANYTEEWVIRPGEADFKYGASPLQATLGLEFGAHFKWDL
                     DNPNTKYAVLTNAAANALGALGGFAVSRFASTDPMLSPHIGAMVGQAAGHAIQYNTPG
                     LKPDTILWWAGATLGAADLNKAEFEVARFTDYPRIWWHAREGAIFPNKADIEHATGAD
                     IRAMEEGIPVGQRHPNPEDVVIDIESNGLPHHNPSNHVDIFDIIQETRV"
BASE COUNT          449 a          283 c          284 g          366 t
ORIGIN      
        1 ggatataaac atgtaataag gatttattat gaacattcaa ccgaccatac aatctggaat
       61 cacctcacaa aacaatcaac atcatcaaac agaacaaata ccctctacac aaataccgca
      121 atccgaatta cctctaggat gccaagctgg atttgttgtt aatattccag atgatataca
      181 gcaacatgca ccggaatgcg gtgaaacaac agctctactg agcttgataa aagataaagg
      241 tctgctctca gggctagacg aatatatagc tcctcacctt gaagaaggat ccataggaaa
      301 aaaaacattg gatatgtttg gtttattcaa tgttacccaa atggcattag agatacctag
      361 ttccgtttca ggcatctctg gtaaatatgg tgtccagcta aacattgtaa aaccagatat
      421 tcatcctaca tcaggtaatt attttttaca gatattccct ctgcatgatg aaataggttt
      481 taattttaaa gaccttcctg gcccgttaaa aaatgcatta agcaacagta atatatcaac
      541 cactgcagtg tcgactattg catcgactgg aacatcagcc actacttcga cggtaaccac
      601 cgagccaaaa gacccaatac catggtttgg attaacagct caagtggttc gtaatcatgg
      661 tgtagaactt cctatagtca aaactgaaaa tggatggaag cttgttggag aaacaccact
      721 tactcctgat gggccgaaag caaattacac ggaggagtgg gttatcagac cgggagaagc
      781 agattttaaa tatggtgcat ctccattaca ggcaactcta gggctggagt ttggcgcaca
      841 tttcaagtgg gatttagata accctaatac taaatatgcc gttcttacca atgctgccgc
      901 aaatgcgctt ggtgctttag ggggatttgc agtatccaga tttgctagta cagatccaat
      961 gttaagtcct catatcggtg caatggttgg gcaagcagca gggcatgcca tacagtataa
     1021 tacccctgga ttaaagccag acactatttt atggtgggct ggtgcgacac tgggggctgc
     1081 cgatttaaac aaggccgagt ttgaagtagc tagattcact gactatcctc gtatatggtg
     1141 gcacgcaaga gaaggagcta ttttccccaa taaagcagat attgaacatg ccacaggtgc
     1201 tgatatacgc gcaatggaag aaggtatccc tgttggacag cggcatccaa atccagagga
     1261 tgtggtaatc gatatcgaaa gcaatggctt accacatcat aatccatcaa atcatgttga
     1321 tatctttgat ataatccaag aaacaagagt ctaagcttta acttgtttat tttaaaataa
     1381 ta
//
LOCUS       DAZKIP010000323         1331 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZKIP010000323 DAZKIP010000000
VERSION     DAZKIP010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.327"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(34..603)
                     /gene="ybfC"
                     /locus_tag="ACHOUK_005029"
     CDS             complement(34..603)
                     /gene="ybfC"
                     /locus_tag="ACHOUK_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfC"
                     /protein_id="HFP9900265.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFP
                     VLNNKAMWRWYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGT
                     LQELINTAEKTAFLGDRFRSYIRAGIYQKKSSDPAQLLAVLDNSIMVKYFKDEKPTYA
                     RMTAHLPNKDESYECLTKIQHELLRSEEK"
     gene            complement(600..>1331)
                     /locus_tag="ACHOUK_005030"
     CDS             complement(600..>1331)
                     /locus_tag="ACHOUK_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP9900266.1"
                     /translation="LISKEGATEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEES
                     GLYYNRHRYYDPLQGRYITQDPIGLKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGG
                     YGARPNKPPTPDPSKFPDIAKQLRLPYPIDQASSAPNVFKTFFRALSPYDYTLYCRKW
                     VKPNLTCTPQDDPQYPGMDTKTASDYLPQTNWPTTQLPPGYTCAEPYLFPDINKPDGP
                     ATAGIDDLGEILAKMKQRTSRGIRK"
BASE COUNT          335 a          299 c          258 g          439 t
ORIGIN      
        1 cgaatggtta atttattttc tcaggaagat gttttatttt tcttcactac gaagcagttc
       61 atgctgtatt tttgttaaac attcgtaaga ctcatctttg ttcgggagat gtgctgtcat
      121 tcgagcataa gttggcttct catctttaaa atatttaacc atgattgaat tatcaaggac
      181 tgccaacaac tgagctggat cactgctctt tttttgatag atacccgctc ttatataact
      241 tctaaaccga tcgccgagaa acgccgtttt ttctgctgta tttatcaact cctgcaacgt
      301 tcctttgatg gcatgatttt cattaaggcg tagtgacccg acacgaatta agagtctcgc
      361 cccgcttggt gtgaatttat tatttttaca tgtacccagt tctgtttgcc aataatactc
      421 accgatgtcc tcattctgat accatctcca catggcttta ttatttaaga caggaaacgc
      481 ggaaaaaata gtacccttag cgaaaatagt aaaacaatca tgatctttag cttgtatttc
      541 gcaatcagct ataacaccaa aactaacaaa tatcatcagc aaaaaaaaca aaactctttt
      601 cattttctta ttcctctcga tgttctctgt ttcattttag ctaaaatttc acccaaatca
      661 tctatccctg ctgttgctgg cccatcgggt ttattaatgt cagggaataa atagggttct
      721 gcacaagtat atcctggtgg taattgagtt gttggccaat ttgtctgtgg caggtaatca
      781 cttgctgtct ttgtatccat ccctggatac tggggatcat cctgtggcgt acaagtcaga
      841 tttggtttta cccacttcct gcaatacagt gtgtagtcgt aagggcttaa tgctctgaag
      901 aatgttttga aaacattagg cgcactactg gcctggtcaa taggatatgg cagtcttaat
      961 tgtttcgcta tgtctggaaa tttacttgga tcgggcgtag gcggtttgtt gggtctcgct
     1021 ccatatccgc cattatttaa gtgtccatat tttgatgcca gtcccattga atctatatac
     1081 tgaaccggat tcaacggata ctgataaaaa ttccatcccc ccttcagtcc aatcggatcc
     1141 tgagtgatat acctcccctg cagcgggtca taatagcggt ggcggttgta atacaggccg
     1201 gactcctcat catactgctg ccccggcagg cggataagct gctgcagctg atgcgggttc
     1261 tcttcattca gcaggttgcc ccactcatcg tattctgcgc accattctgt tgccccttcc
     1321 ttactgataa g
//
LOCUS       DAZKIP010000324         1324 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZKIP010000324 DAZKIP010000000
VERSION     DAZKIP010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.328"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            297..458
                     /locus_tag="ACHOUK_005031"
                     /pseudo
     CDS             297..458
                     /locus_tag="ACHOUK_005031"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            442..507
                     /locus_tag="ACHOUK_005032"
     CDS             442..507
                     /locus_tag="ACHOUK_005032"
                     /inference="COORDINATES: protein motif:HMM:NF033646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAST1 family heat-stable enterotoxin"
                     /protein_id="HFP9900267.1"
                     /translation="MWCATACASCHGRNTKPSLAT"
     gene            623..880
                     /locus_tag="ACHOUK_005033"
     CDS             623..880
                     /locus_tag="ACHOUK_005033"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9900268.1"
                     /translation="MATFFAYPADIRKVIYTTNAIESLNSVIRHAIRKRKVFPTDESV
                     KKVVWLAIQAASQKWTMPLRDWRMAMSRFIIEFGDRLDGHF"
     gene            complement(927..>1324)
                     /gene="nleF"
                     /locus_tag="ACHOUK_005034"
     CDS             complement(927..>1324)
                     /gene="nleF"
                     /locus_tag="ACHOUK_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector caspase inhibitor NleF"
                     /protein_id="HFP9900269.1"
                     /translation="NNELEEIFQELSAFVSGLMDKRNSVFEVRNENTDEVVGALRAGM
                     TIEDRDSYIRDLFFLHSLKVKIEESRQGKEDSKCKVYNLLCPHHSSELYGDLRAMKCL
                     VEGCSDDFNPFDIIRVPDLTYNKGSLQCG"
BASE COUNT          389 a          314 c          267 g          354 t
ORIGIN      
        1 aaaaacaact ttttatcttt ttgtttcgct caaattagta taaaaagccg aacaacaaac
       61 aataaaaaac cattaacatc aatagattat gatggcttca atctaaataa taggctatat
      121 aatactgcaa gacacaacac atgctgtcac tatgagtcaa ctatttagat gatattagtg
      181 acctgtttca gagcattagc gcaaggcgat tctttgtctt cttgcactaa ttttttatca
      241 taaaaatgtt cctagcactg ggcatcaata tcgcaggtca gaaagagctc ctggggatgc
      301 ggctggccga aaatgaaggg gcgaatttct ggttcaatgt gctgactgaa ctgaaaaacc
      361 gcggtctgaa cgatatcctc atcgcctgtg tgtatggcct gaaagaattc ccggaggccc
      421 gcatccagtt atgcatcgtg catatggtgc gcaacagcat gcgcttcgtg tcatggaagg
      481 aatacaaagc cgtcactcgc gacctgaaag cgattagcct cccacagaag aggcaggcca
      541 gcaggcactg gaagcgtttg ctgcggcctg ggactgccgc tatccgcaga taagccggtg
      601 ctagctgtca aactggacta acttggcgac gtttttcgct tatccggcag atatccgcaa
      661 agtgatctac acaacgaacg ccatcgagtc gctgaacagt gtgatccggc atgccatcag
      721 gaaacgcaag gtgttcccga cggacgagtc agtaaaaaaa gtggtgtggc tggcaatcca
      781 ggccgcgtca cagaaatgga caatgccgtt aagggactgg cgaatggcaa tgagccgctt
      841 tattatcgag tttggtgacc gtcttgacgg tcacttttaa gaaaaggcat ttacacagaa
      901 tcctaaacag gctcacaggc ctctaatcat ccacattgta aagatccttt gttgtaagta
      961 agatctggta ccctaataat atcaaaagga ttaaaatcat cactgcatcc ttccacgagg
     1021 catttcattg ctcgtagatc accatatagc tctgaagagt gatgcggaca tagcagatta
     1081 taaactttac atttcgaatc ttctttgcct tgtctacttt cctcaatttt tactttcaat
     1141 gaatgcagaa aaaaaagatc cctgatataa ctatccctgt cctctatcgt cattcccgcc
     1201 ctcagcgctc cgacaacctc atcagtattt tcatttctca cctcaaatac actatttctc
     1261 ttatccatca gcccacttac aaaagcactt agctcttgaa aaatttcctc aagctcatta
     1321 ttat
//
LOCUS       DAZKIP010000325         1320 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZKIP010000325 DAZKIP010000000
VERSION     DAZKIP010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.329"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..361
                     /locus_tag="ACHOUK_005035"
     CDS             <1..361
                     /locus_tag="ACHOUK_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP9900270.1"
                     /translation="DGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            632..1201
                     /locus_tag="ACHOUK_005036"
     CDS             632..1201
                     /locus_tag="ACHOUK_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFP9900271.1"
                     /translation="MNMMAVPFHGNSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKQRNMVSMPLRKLAGWLQTINPNKVKPEIRDKVIRYQEEC
                     DDVLYEYWTKGFVVNPRKMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKAAHVS
                     KIRTLVNEANMLIDFVLADTGKGKITKAD"
     gene            1201..>1320
                     /locus_tag="ACHOUK_005037"
     CDS             1201..>1320
                     /locus_tag="ACHOUK_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900272.1"
                     /translation="MGWLMISDKLITLVKSLCVLVGISFLVMLVAIFFSTAWRV"
BASE COUNT          362 a          278 c          355 g          325 t
ORIGIN      
        1 ggatggtaag cctgttattc ctggcatgaa gctgtcgaag gaaaaatgcg accgggttaa
       61 cgccattgag cgtgataagg cgctggcatg ggtggagaaa aacatcagag tgccgctgac
      121 cgaaccccag aaagcgggga tcgcgtcatt ctgtccgtac aacattggcc ccggtaagtg
      181 tttcccgtcg acgttttaca gacggattaa tgcaggagat cgaaaaggtg cctgcgaagc
      241 gattcgctgg tggattaagg acggtggcag ggactgccgt attcgctcaa ataactgtta
      301 cggtcaggta tcccgtcgtg accaggagag cgcgctggcg tgctggggaa tcgacagata
      361 agcagaatat tttgctgaaa aatgaggttt gcttacatgg acggataaca cgaaatcctg
      421 caaattggca aaatgtaagt gaataaagtc aaaacagttg tttaacactc aggcaccgta
      481 atgatgcctt tgtcatttct gcgcatctca cgcgcatctc acaacacaga acctttcagg
      541 atgacccttg aggataccgg tttggctgtc ggtgcctttc tgtgggctgg attcctgtga
      601 gacaaggttc atcactaaaa ggaaataacc gatgaatatg atggccgtgc cgtttcacgg
      661 caactctctt tatgtagtta accataatgg cgaaccatac gttcccatga aacctgtcgt
      721 tgcggggatg gggctggcct ggcaatcaca gttggctaag ttaagacagc gttttgcgtc
      781 aactataacg gaaatcgtta tggttgctga ggatgggaaa caacgcaata tggtgtccat
      841 gccacttcga aaacttgccg gctggctaca aaccattaat cccaacaaag taaaacccga
      901 aatccgcgat aaggtcatcc ggtatcagga agagtgcgac gatgttcttt acgagtactg
      961 gacgaagggt tttgtcgtta atccccgtaa aatgagcgtg atggaagaac tcaaccaggc
     1021 ttgtgctgac atgaaacggg ataaaaacat tgccagtgtg tttgctaccg ggctgaatga
     1081 gtggaaacag gttaaagccg cgcatgtatc aaaaatccgt acgctggtaa atgaagcgaa
     1141 tatgctgatt gattttgtcc tggctgatac aggcaaaggg aaaataacaa aggcggattg
     1201 atggggtggc taatgatatc agataaactc ataacgctgg tgaagagcct ctgtgtactt
     1261 gtcggcattt catttttagt catgctggtt gccattttct tttccaccgc ctggcgagtc
//
LOCUS       DAZKIP010000326         1300 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZKIP010000326 DAZKIP010000000
VERSION     DAZKIP010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.330"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..>1300)
                     /gene="espX6"
                     /locus_tag="ACHOUK_005038"
     CDS             complement(<1..>1300)
                     /gene="espX6"
                     /locus_tag="ACHOUK_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector pentapeptide repeat protein EspX6"
                     /protein_id="HFP9900273.1"
                     /translation="CNVKTEQLQQFIKIMEQKAQLPIYIDKNNLKESIFSVLHNDPQQ
                     VDKDQHLPCEKFLKHACKSSNSFEVKLDATHQYQHLNNFMISFDPVENQLTIRDNNNK
                     TETFSFTNLQWENLLQYYKENHQQPNIAGSRNLTDNIDKIKNTISTSEIIECASPEIR
                     SSVLNDLYSIANFLPDNNLTPNESWKRFCETCERFYVAQKSITGDKSERLTRKLSISD
                     AGITMTFKIGDVVINTISTAIPEDATGQRCIEGLNLAEMDLTDIDLSKMALRNVNFNG
                     SILRNAKFSGTICEGVDFTDCDLRNAEFENASLENNDFRKVRHLTYVNFKNANLRNSN
                     FNGKVLTGVTFTGSDLSNAYLEHIDFTTVILYETSKIPGIPGTPQIPGTPKVILTGAI
                     LNYSDLSGKDLSEYNLTGILCMYTNFSNANLTNCKISNANF"
BASE COUNT          360 a          222 c          255 g          463 t
ORIGIN      
        1 aaaagtttgc attagagatt ttacaatttg ttaaattagc gtttgaaaag ttggtataca
       61 tgcagagaat accagtaaga ttatattctg aaagatcttt tcccgataga tcggaataat
      121 ttagtattgc gccagtaaga attactttag gtgttcccgg tatttgaggt gttccaggta
      181 ttccaggtat tttagatgtt tcatatagaa tcacggttgt gaaatctatg tgttcaagat
      241 acgcgttact aaggtcactt ccagtaaagg ttacgccagt gagaactttt ccgttgaagt
      301 tactgtttcg tagatttgcg tttttgaaat ttacataagt caagtggcga actttacgaa
      361 aatcattatt ttctaatgag gcattttcga attctgcatt acgcagatca caatcggtaa
      421 aatccacgcc ttcacagatc gtaccggaga acttggcatt tctaagaatg ctgccattaa
      481 aattgacatt ccttagcgcc attttcgaca agtctatgtc ggttaaatcc atctctgcta
      541 aattcaaccc ttcgatacac cgttgacccg ttgcatcttc aggaatagca gtgctaatag
      601 tattgatgac aacatcacct atcttgaagg tcattgtaat tcctgcatca gagatagaga
      661 gttttcgcgt aagacgttca ctcttatctc cagtgatact cttctgagca acgtaaaagc
      721 gctcgcatgt ctcacaaaat cttttccagc tctcatttgg ggtcagatta ttgtccggga
      781 ggaaattagc aatgctataa agatcgttca ggacgctact tcttatttca ggagaggcgc
      841 attcaataat ttcagaggtg gatattgtat ttttaatttt atctatatta tccgtgagat
      901 ttcgtgatcc tgctatattt ggctgctggt ggttttcttt gtagtattgc agcaaatttt
      961 cccattgtaa gtttgtaaac gagaaagttt cagtcttgtt attgttatcc cgtatggtta
     1021 attgattttc tactgggtca aaagaaatca tgaagttatt caggtgttga tattgatgag
     1081 tggcatctaa tttcacttca aatgaatttg aacttttgca ggcatgtttt aaaaactttt
     1141 cacagggaag gtgttgatct ttatctactt gttgtgggtc attgtgcaaa acagagaaaa
     1201 tactctcttt caaattgttc ttgtcaatat agattggtag ttgcgctttt tgttccatta
     1261 ttttaataaa ctgctgcagt tgctcggttt taacattgca
//
LOCUS       DAZKIP010000327         1289 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZKIP010000327 DAZKIP010000000
VERSION     DAZKIP010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.331"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..1182
                     /locus_tag="ACHOUK_005039"
     CDS             <1..1182
                     /locus_tag="ACHOUK_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP9900274.1"
                     /translation="RRMAKRVWRRERDLTGWMSLSRKPEVTWYGWDGDRLTTVQTDTT
                     RIQTVYEPGSFTPLIRVETENGEREKAQRRSLAETLQQEGSENGHGVVFPAELVRLLD
                     RLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEPEYTPARKVHFYHCDHRGLPLALI
                     SEDGNTAWRGEYDEWGNQLNEENPYYLHQPYRLPGQQHDEESGLYYNRNRYYDPLQGR
                     YITQDPIGLAGGWNLYNYPLNPIIRMDPLGLYNLYQLLYDVWHDDSYGTSSIDITGSG
                     DLISLGGHAGLGVAFAKKKGEMLSDICIYATACGHAGIGGGINAAITYSETKSLPTSG
                     VSNSVGVTVGGGVGGHFAYTYVVDVDNPESSTESVGIGAGVDASVMTLACRTWQECWV
                     N"
BASE COUNT          330 a          258 c          404 g          297 t
ORIGIN      
        1 cggcgaatgg caaaacgggt ctggcggcgg gagcgtgacc tgacggggtg gatgtcgctg
       61 tcgcgtaaac cggaggtgac gtggtatggc tgggacggag acaggctgac gacggtgcag
      121 actgacacca cacgtatcca gacggtatac gagccgggaa gcttcacgcc gctcatccgg
      181 gtcgagacag agaacggcga gcgggaaaaa gcgcagcggc gcagcctggc agagacgctc
      241 cagcaggaag ggagtgagaa cggccacggc gtggtgttcc cggctgaact ggtgcggctg
      301 ctggacaggc tggaggaaga aatccgggca gaccgcgtga gcagtgaaag ccgggcgtgg
      361 cttgcgcagt gcgggctgac ggtggagcaa ctggccagac aggtggagcc ggaatacaca
      421 ccggcgcgaa aagttcattt ttaccactgc gaccaccggg gcctgccgct ggcgctcatc
      481 agcgaagacg gcaatacggc gtggcgcggg gagtatgatg aatggggcaa ccagcttaat
      541 gaagagaacc cgtattacct gcaccagcca taccgtctgc cggggcagca gcatgatgag
      601 gaatcagggc tgtactataa ccggaaccgg tactatgacc cgctacaggg gaggtatatt
      661 acacaagacc ccattgggct ggcgggggga tggaatctgt ataattaccc actgaatccg
      721 ataataagga tggatccttt gggtttgtat aatttatatc aattattata tgatgtttgg
      781 catgatgatt catatggaac atcatcaatt gatattactg gcagtggaga tctaatatca
      841 ttaggtggtc atgcaggact tggcgttgcg tttgctaaaa agaaaggtga aatgttatct
      901 gatatttgta tttatgctac agcatgcgga catgcaggaa ttggtggtgg gataaatgcg
      961 gctatcacat attctgagac caagtcttta cctacatcgg gagtcagcaa ttcagtaggt
     1021 gtaacggttg gcggcggagt tggggggcat tttgcgtata cttatgtagt ggatgttgat
     1081 aatccagaat catcgacaga atctgttggt atcggtgcag gtgttgacgc ttcagttatg
     1141 actctggctt gtagaacgtg gcaagaatgc tgggtcaatt aacaggagga aatatgaaaa
     1201 aatatatatt tttagtttta tttttattgt atggttgttc tccagtggac atagagccag
     1261 ctagacaaga agttaatatc tttcgtggt
//
LOCUS       DAZKIP010000328         1277 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZKIP010000328 DAZKIP010000000
VERSION     DAZKIP010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.332"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            18..758
                     /locus_tag="ACHOUK_005040"
                     /pseudo
     CDS             18..758
                     /locus_tag="ACHOUK_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415922.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS30 family transposase"
     gene            809..1111
                     /gene="bamE"
                     /locus_tag="ACHOUK_005041"
     CDS             809..1111
                     /gene="bamE"
                     /locus_tag="ACHOUK_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFP9900275.1"
                     /translation="MKNETQESVKTKIVKGKTTKQDVLASFGEPDSRSLIDGEEQWSY
                     TMYNSQSKATSFIPVVGLLAGGADSQTKSLTVSFKGEKVSTYIFNAGTSNVKTGIF"
     gene            complement(1187..>1277)
                     /locus_tag="ACHOUK_005042"
     CDS             complement(1187..>1277)
                     /locus_tag="ACHOUK_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900276.1"
                     /translation="SSLKENTETVEMNSKVWGRLKKIITFFKK"
BASE COUNT          404 a          278 c          287 g          308 t
ORIGIN      
        1 ttgcggaggt tttttgaatg agacgaacat ttacagcaga ggaaaaagcc tctgtttttg
       61 aactatggaa gaacggaaca ggcttcagtg aaatagcgaa tatcctgggt tcaaaacccg
      121 gaacgatctt cactatgtta agggatactg gcggcataaa accccatgag cgtaagcggg
      181 ctgtagctca cctgacactg tctgagcgcg aggagatacg agctggtttg tcagccaaaa
      241 tgagcattcg tgcgatagct actgcgctga atcgcagtcc ttcgacgatc tcacgtgaag
      301 ttcagcgtaa tcggggcaga cgctattaca aagctgttga tgctaataac cgagccaaca
      361 gaatggcgaa aaggccaaaa ccgtgcttac tggatcaaaa tttaccattg cgaaagcttg
      421 ttctggaaaa gctggagatg aaatggtctc cagagcaaat atcaggatgg ttaaggcgaa
      481 caaaaccacg tcaaaaaacg ctgcgaatat cacctgagac aatttataaa acgctgtact
      541 ttcgtagccg tgaagcgcta caccacctga atatacagca tctgcgacgg tcgcatagcc
      601 ttcgccatgg caggcgtcat acccgcaaag gcgaaagagg tacgattaac atagtgaacg
      661 gaacaccaat tcacgaacgt tcccgaaata tcgataacag acgctctcta gggcattggg
      721 agggcgattt agtctcaggt acaaaaaact ctcatataga ttcgtttttc tgaccttggc
      781 aggttgttct tcagtaggca accagtcatt gaaaaatgag acgcaggaaa gtgtgaaaac
      841 caaaattgtt aaaggcaaaa ctacaaaaca ggacgtgtta gcatcgttcg gtgaacctga
      901 cagccgttct ttgatcgatg gtgaagaaca atggtcatac actatgtata acagccagtc
      961 caaagcaacc tctttcatcc ccgttgtggg actgcttgca ggtggcgcag actcacaaac
     1021 taaatctctg acagtttctt tcaaaggcga aaaagtcagc acatacatct ttaatgctgg
     1081 aacaagcaac gtgaagactg gcatttttta gataaaagac aaaaccagca tcaagggaaa
     1141 atttagcccg cagttgatac ctacgggctt tttacagaaa cataaatcac tttttaaaga
     1201 aagtgattat ctttttcagc ctcccccaaa ctttactgtt catttctaca gtctcagtat
     1261 tttcctttaa agaggat
//
LOCUS       DAZKIP010000329         1268 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZKIP010000329 DAZKIP010000000
VERSION     DAZKIP010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.333"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>1268
                     /locus_tag="ACHOUK_005043"
     CDS             <1..>1268
                     /locus_tag="ACHOUK_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFP9900277.1"
                     /translation="DGVETYGVWGLSLPSLRRRLFRCVSIRENTDGTFAITAVQHVPE
                     KEAIVDNGASFEPQSGTLNSVIPPAVQHLTVEVSAADGQYLAQAKWDTPKVVKGVSFM
                     LRLTVAADDGSERLVSTARTTETTYRFTQLAPGNYRLTVRAVNAWGQQGDPASVSFRI
                     AAPAAPSQIELTPGYFQITAVPKLAVYDPTVQFEFWFSEAKIADTSQVETSARYLGTG
                     SQWSVSGPHIKPGKDFWFYVRSVNLVGKSAFVEVSGQPSNDGEGYLEFFREKIGKLHL
                     AQGLWELIDNSQLADEMAEMKTSITETRNEITQTVSKTLEDQSATIQQIQRVQKDTND
                     DLAALYMLKVQKTKDGIPYVAGIGAGIEDTDGQPLSNILLLADRIAMINPESGNSTPL
                     FVAQGNQLFMNDVFLKRLFAVSITSSGNPPA"
BASE COUNT          302 a          322 c          403 g          241 t
ORIGIN      
        1 gatggtgtgg agacatacgg tgtatgggga ctctccctgc cgtcactgcg tcgtcgcctg
       61 ttccgctgtg tctccatccg ggaaaacacg gacggcacct ttgccatcac ggcggtgcag
      121 cacgtaccgg aaaaagaagc catcgtggat aacggggcca gctttgagcc gcagtcaggc
      181 accctgaaca gcgttattcc accggcagtg cagcacctga cggtggaggt gagcgcggct
      241 gacggtcagt atctggcaca ggcgaaatgg gacacgccga aggtggtgaa gggcgtgagc
      301 tttatgcttc gcctgaccgt ggccgcggat gacggcagtg agcggctggt cagcacggcc
      361 cggacgacgg aaaccacata ccgcttcacg caactggcgc cggggaacta caggctgaca
      421 gtccgggcgg taaatgcgtg ggggcagcag ggcgatccgg cgtcggtatc gttccggatt
      481 gccgcaccgg cagcgccgtc acagattgag ctgacaccgg gctattttca gataacggcg
      541 gtcccgaaac tggctgtata tgacccgacg gtacagtttg aattctggtt ttcggaggca
      601 aaaatcgcag acacatctca ggtggaaacc tctgcccgtt atctggggac cggcagtcag
      661 tggagtgtat ccggcccgca cattaagccc gggaaggatt tctggtttta cgtgcgcagc
      721 gtcaacctgg tggggaaatc tgcgtttgtg gaagtcagcg ggcagcccag caatgatggt
      781 gaagggtatc tggaattttt ccgggaaaaa ataggaaaac tgcatctggc tcaggggttg
      841 tgggaactga tagataacag ccagcttgcg gatgagatgg cggagatgaa gaccagcatc
      901 acggaaaccc gcaatgaaat cacacagacg gtcagtaaaa cgctggagga ccagagcgcc
      961 accatacagc agatacagcg cgtgcagaag gacacaaatg atgacctggc tgcgctgtac
     1021 atgctgaagg ttcaaaaaac gaaagacggc attccctatg tggccgggat tggtgcaggg
     1081 attgaggata ctgatggcca gccactgagc aacatactgc tgctggctga ccgtatcgcg
     1141 atgataaatc cggagagcgg caacagcacg ccgttatttg tggcgcaggg gaatcagctg
     1201 ttcatgaacg acgtgttcct gaaacgactg tttgcggtga gcatcacatc atccggcaat
     1261 cctccggc
//
LOCUS       DAZKIP010000330         1267 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZKIP010000330 DAZKIP010000000
VERSION     DAZKIP010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.334"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            1..1267
                     /locus_tag="ACHOUK_005044"
                     /pseudo
     CDS             1..1267
                     /locus_tag="ACHOUK_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
BASE COUNT          307 a          327 c          397 g          236 t
ORIGIN      
        1 gatggcgtgg agacatacgg tgtgtggggg ctctccctgc cgtcactgcg tcgtcgcctg
       61 ttccgctgtg tctccatccg ggaaaacacg gacggcacct ttgccatcac ggcagtgcag
      121 cacgtaccgg aaaaagaagc catcgtggat aacggggcgc actttgacgg cgaccagagc
      181 ggcaccctga acagcgtcat ccctccggca gtgcagacct gacggtggag gtgagtgcag
      241 ctgacagcca gtatctggcg caggcgaaat gggacacgcc gcgggtggtg aagggcgtgc
      301 gcttcagtct gcgcctgacc agtggaagcg gtcaggacag ccgtctggtg accaccgcca
      361 tcactgcgga tacagagcat cgtttcagtg gtctgccgct cggggaatac accctgacag
      421 tcagggcaat taacagttat ggccagcagg gggaaccggc catcaccacc ttccggatta
      481 acgcgccagc aaaacccgcc accattgaac tgacgccggg gtattttcag ataacggcgg
      541 taccggtgct ggcggtgtat gacccgacgg tgcagtttga gttctggttt tcggaaaaac
      601 gcatcacgaa cacggcacag gtggaaaaat ctgcccgtta tctggggagc ggcagtcagt
      661 ggactgtcca gggaagccgg attaagccgg ggacggattt ctggttttac gtgcgcagcg
      721 tcaacctggt ggggaaatct gcgtttgtgg aagtcagcgg gcagcccagc aatgatggtg
      781 aagggtatct ggaatttttc cgggaaaaaa taggaaaact gcatctggct caggggctat
      841 gggagctgat agacaacagc cagcttgcgg atgagatggc ggagatgaag accaccatca
      901 cggaaacccg caatgaaatc acacagacgg tcaacaaaac gctggagaac cagagcgcca
      961 ctatacagca gatacagcgc gtgcagaagg acacaaatga tgacctggct gcgctgtaca
     1021 tgctgaaggt tcaaaaaacg aaagacggca ttccctatgt ggccgggatt ggtgcaggga
     1081 ttgaggatac tgatggccag ccactgagca acatactgct gctggctgac cgtatcgcga
     1141 tgataaatcc ggagagcggc aacagcacgc cgttatttgt ggcgcagggg aatcagctgt
     1201 tcatgaacga cgtgttcctg aagcgactgt ttgcggtgag tatcacctcg tccggcaatc
     1261 ccccgac
//
LOCUS       DAZKIP010000331         1263 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZKIP010000331 DAZKIP010000000
VERSION     DAZKIP010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.335"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            151..1110
                     /locus_tag="ACHOUK_005045"
     CDS             151..1110
                     /locus_tag="ACHOUK_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HFP9900278.1"
                     /translation="MKKSVIAGVFIALSFTTCSAIANSLALSLANDDAGKFQPILNDI
                     YGNKHENRDDYSQGLFLGYSHDISDSSQLSLHIAQDIYSPSGSNKRHNTAVTGDRAFS
                     AYTHTGIEWNSLANDWIRYRLGTDIGVVGPDAGGQKVQNKAHEIIGAEKYHAWDDQIE
                     NRYGYTVKGMLSMTPSMDILGANVGLYPEVSAVTGNLFQYVAYGATIAIGNDKTFNSD
                     NGFGLLAPRGLMHMSDTSGFKYKIFAGMERRDVNRNYTLEGKTIQTKQTTVSLNKTVD
                     EYQVGATIGYAPVAFTLAFNKVTSEFKTGDDYSFINGAITFFF"
BASE COUNT          457 a          237 c          230 g          339 t
ORIGIN      
        1 acattaaaag ataataaaaa ccgcaataaa aaacgaataa atcaattgtc tcacgcaatt
       61 ataaaacatc atattgatta cgcaccttgc attacaaact catgtatgta aaatacgcgc
      121 accattcaaa aaaaaggaag acaataacat atgaaaaaaa gtgtcatcgc tggcgtcttt
      181 attgctctgt catttaccac gtgttcagct atcgcgaaca gccttgcatt atcattagca
      241 aatgatgatg cagggaagtt tcaaccaata cttaatgata tttatggcaa taaacatgaa
      301 aacagagatg attactcaca aggcttattt ctgggatata gccacgatat ctcagactcg
      361 agccaattat ctctccatat tgcgcaagat atttactctc catcaggcag taataaaaga
      421 cacaacacag ctgtaactgg agacagagct tttagtgcat acactcacac tggtattgaa
      481 tggaactccc ttgcgaatga ctggattcgc tatcgattag gtactgacat aggtgttgtt
      541 ggccccgacg caggcggtca gaaagtacaa aataaagctc atgagattat tggggcagaa
      601 aaatatcatg catgggatga tcaaatagag aatcgctacg gttatactgt aaaagggatg
      661 ctatccatga caccaagtat ggatatttta ggtgctaatg ttggattata ccctgaagtt
      721 tctgctgtta ctggaaactt atttcaatat gtagcatatg gcgcaaccat tgccattggt
      781 aatgataaaa ccttcaattc ggataatggc tttggtctgc tggctccccg tggtttaatg
      841 catatgtccg atacaagcgg attcaaatac aagatttttg caggtatgga aagacgagat
      901 gtcaatcgca actatactct cgaaggaaaa acaatacaga cgaaacaaac aacagtatcg
      961 ctaaacaaaa ctgttgatga atatcaagtt ggcgcaacaa ttgggtatgc acctgtagcc
     1021 ttcacactag catttaataa agtaacatca gaattcaaga caggggatga ctattcattt
     1081 ataaatggag caatcacctt ctttttttaa ctgaattgaa ttcaatcaaa ataacataag
     1141 tccaacaaaa acataaagtg cgaaatgaat gccagctcca tttatttcgc actataaaag
     1201 attaaaagtt gcaataaaat aataaaatga ctcagttacg aaaaccaata aactgtggcc
     1261 agt
//
LOCUS       DAZKIP010000332         1252 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZKIP010000332 DAZKIP010000000
VERSION     DAZKIP010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.336"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            44..1189
                     /gene="espG"
                     /locus_tag="ACHOUK_005046"
     CDS             44..1189
                     /gene="espG"
                     /locus_tag="ACHOUK_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspG"
                     /protein_id="HFP9900279.1"
                     /translation="MINGLNNDSASLVLDAAMKVNSGFKKSWDEMSCAEKLFKVLSFG
                     LWNPTYSRSERQSFQELLTVLEPVYPLPNELGRVSARFSDGSSLRISVTNSELVEAEI
                     RTANNEKITVLLESNEQNRLLQSLPIDRHMPYIQVHRALSEMDLTDTTSMRNLLGFTS
                     KLSTTLIPHNAQTDPLSGPTPFSSIFMDTCRGLGNAKLSLNGVDIPANAQKLLRDALG
                     LKDTHSSPTRNVIDHGISRHDAEQIARESSGSDKQKAEVVEFLCHPEAATAICSAFYQ
                     SFNVPALTLTHERISKASEYNAERSLDTPNACINISISQSSDGNIYVTSHTGVLIMAP
                     EDRPNEMGMLTNRTSYEVPQGVKCIIDEMVSALQPRYAASETYLQNT"
BASE COUNT          408 a          263 c          235 g          346 t
ORIGIN      
        1 tgttgccaaa ttgttcatta caaaccagat aggagaatct ctcatgataa atggacttaa
       61 taatgactcc gcatctttag ttttagatgc tgcaatgaaa gttaattctg ggtttaaaaa
      121 aagctgggat gagatgtcat gcgctgaaaa gttatttaaa gtacttagtt ttggtttatg
      181 gaatccaacg tacagtcgta gtgaaagaca atcatttcaa gagttgttaa ccgttttaga
      241 gcctgtatat ccacttccca atgaattagg cagagtatct gctcgttttt cagatggttc
      301 atccttaaga atttccgtca ctaacagcga acttgttgaa gccgagattc gcacagcaaa
      361 taatgaaaag attactgtgc tcctggagtc aaacgaacaa aataggttat tacaatcttt
      421 acccatcgat cgccacatgc catacattca ggttcatcgt gccttatctg agatggacct
      481 gactgatact acctcaatgc gcaatctact tggttttacg tcaaaactat caacaacctt
      541 gattcctcat aatgctcaaa cagatccgct ttccgggcct acaccattca gctctatctt
      601 tatggataca tgtcgaggac tcggcaatgc aaagctttca ctcaatggtg ttgatatacc
      661 tgcaaatgca caaaaattgc ttcgcgatgc actaggactt aaagacacac attcatcacc
      721 aacccggaat gttatagatc atggtatttc tcgccatgat gcagagcaaa tagcaagaga
      781 aagcagcggc agtgataaac agaaagctga agttgtggaa tttttatgcc atccagaagc
      841 agcaacggcc atatgctcgg ctttctatca atctttcaat gtgccagcct taacgttgac
      901 acatgaaagg atctctaaag ccagtgaata caatgcggaa agatcattag atacacctaa
      961 cgcttgcatt aacatcagta tctctcaatc atcagatgga aacatttatg ttaccagcca
     1021 tactggggtt ctgataatgg cgccagaaga ccgccccaac gagatgggca tgttgacgaa
     1081 caggacttct tatgaagtgc cgcaaggtgt gaaatgtata atcgatgaaa tggtaagtgc
     1141 gctacaacca aggtatgccg catctgaaac gtacttacaa aacacttaaa ataaaacttt
     1201 attaatcaaa actaataata gaaaccacac ccataattat taacaattta tt
//
LOCUS       DAZKIP010000333         1238 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZKIP010000333 DAZKIP010000000
VERSION     DAZKIP010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.337"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(218..385)
                     /locus_tag="ACHOUK_005047"
     CDS             complement(218..385)
                     /locus_tag="ACHOUK_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFP9900280.1"
                     /translation="MTVKLRLAVAALLLFLVVMVDFTSRIMSVLADGVLVCGIVVLLW
                     PVIKRNSLHNA"
     gene            complement(382..813)
                     /locus_tag="ACHOUK_005048"
     CDS             complement(382..813)
                     /locus_tag="ACHOUK_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance protein"
                     /protein_id="HFP9900281.1"
                     /translation="MFGIFKKKTRRAATEIKKFEKRDLAQVVINAAYLVACADGECEA
                     SEKAKIEQVLRNQPALSAFTSEINAISATIIGQLDTNFKTGRRAALREIEDVKHDTRE
                     AEDVLDVAVAIAEADGEIEPEERKVLEEIAGVLGLRLENHL"
     gene            complement(983..1059)
                     /locus_tag="ACHOUK_005049"
     tRNA            complement(983..1059)
                     /locus_tag="ACHOUK_005049"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1023..1025),aa:Arg,seq:tct)
     gene            complement(1160..1235)
                     /locus_tag="ACHOUK_005050"
     tRNA            complement(1160..1235)
                     /locus_tag="ACHOUK_005050"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1200..1202),aa:Ile,seq:cat)
BASE COUNT          302 a          363 c          294 g          279 t
ORIGIN      
        1 cgcgccatgc gcagccggaa ataaagggat aacgatgata gtttgagaaa aacagaaaca
       61 acacttttgt ggcaaagcat ggtgccgggt gcctcccggt gaattcagta tcagcacctg
      121 aatccgcgat tatcccatat acctggttgc tgatcgcccc tccgcacagg gggattcacc
      181 atgcagaagt gtttttaata aacagcaaac aaaaaaatca agcattatgc aggctgtttc
      241 tttttatcac cggccacagc aataccacaa tgccgcagac cagcacccca tccgccagca
      301 ccgacatgat tctgctggtg aaatccacca tcaccaccag aaacagcagg agtgcagcca
      361 cagccaggcg cagttttacc gtcacaggtg attctccaga cgaagaccca gaacaccggc
      421 aatctcttcc agcaccttgc gctcttccgg ctcaatttcg ccgtctgcct ccgcaatggc
      481 caccgccaca tccagcacat cttccgcttc acgcgtatcg tgtttcacat cctcaatctc
      541 acgtaacgcc gcacgacgac cagttttaaa gttcgtatcc agctgaccga taatggttgc
      601 gctaatcgca ttaatttctg acgtaaacgc ggacagcgca ggctgattac gcagtacctg
      661 ttcgatcttc gctttctcgg atgcctcaca ttcaccatct gcacaggcca ccaggtatgc
      721 ggcgttaatc accacctgtg ccagatcgcg tttttcaaac tttttaattt ccgttgccgc
      781 tctgcgggtt ttctttttga agattccaaa catcgtgacg ttcctttggg tgggtgagcc
      841 aacgcccggg agcgatctgc ccacagagaa agtcacactg accactccgt aagctcaccc
      901 ccgaaaggct ctgtggttgg tatgcgccgg gcgtggcgca gatacaaaaa aggcccgccg
      961 aagcgagcct ggaaaataag tgtggcgcgt tgtagtggag tcgaaccact gaccgattgc
     1021 ttagaaggca attgctctgt ccggctgagc taacaacgca gaataccgat aatggaccgc
     1081 catcggggac ccggccccgc accaacaacc ctgttatggt gtcgtctgct cttcctgata
     1141 agctaatggc ggtttgtgat ggtggccctt gctggatttg aaccagcgac ctggcgatta
     1201 tgagtcgctc gctctcacca ctgagctaaa gggccgag
//
LOCUS       DAZKIP010000334         1235 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZKIP010000334 DAZKIP010000000
VERSION     DAZKIP010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.338"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            112..351
                     /locus_tag="ACHOUK_005051"
     CDS             112..351
                     /locus_tag="ACHOUK_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
                     /protein_id="HFP9900282.1"
                     /translation="MADWNGYIMDISKQFDQGVDDLNQQVEKALEDLATNPSDPKFLA
                     EYQSALAEYTLYRNAQSNVVKAYKDLDSAIIQNFR"
     gene            371..703
                     /gene="sctI"
                     /locus_tag="ACHOUK_005052"
     CDS             371..703
                     /gene="sctI"
                     /locus_tag="ACHOUK_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HFP9900283.1"
                     /translation="MSVSNMPPIDRAEQSTAHEIQQAKVIDLNDRVLNLDNPDDKMIS
                     AFANYAVQTENWQQNALQALTSDKKGLTPEKLLVLQDHVLNYNVEVSLVGTLARKIVA
                     AVETLTRS"
     gene            700..>1235
                     /locus_tag="ACHOUK_005053"
     CDS             700..>1235
                     /locus_tag="ACHOUK_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HFP9900284.1"
                     /translation="MKYISLLLFILLLCGCKQQELLNHLDQQQANDVLAVLQRHNINA
                     EKKDQGKTGFSIYVEPTDFASAVDWLKIYNLPGKPDIQISQMFPADALVSSPRAEKAR
                     LYSAIEQRLEQSLKIMDGIVSSRVHVSYDVDTGDSGKTALPIHISVLAVYEKDINPEI
                     KINDIKRFIVNSFASVQY"
BASE COUNT          414 a          212 c          252 g          357 t
ORIGIN      
        1 tgaatttgaa aaatcaggac acaaataata gttcatttag ctacggagat aaccgattcg
       61 agaagtctca aggtagcaag tggacatttc aggaataatt gaggaggact tatggcagat
      121 tggaatggtt atattatgga catcagcaaa caatttgatc agggcgttga tgatctgaac
      181 cagcaagttg aaaaagcgtt ggaggattta gcaaccaatc cttccgaccc gaaattcctt
      241 gccgaatatc aaagtgcatt agctgagtat acattatatc gaaatgcgca atctaacgtt
      301 gtaaaagcgt ataaagatct tgattcggca atcatacaaa acttccgcta aactggataa
      361 ggattttatt atgtcagtaa gcaatatgcc acctattgat agagctgagc aatcaactgc
      421 tcatgaaata cagcaagcga aagtgattga tttaaatgac cgggttctca atcttgacaa
      481 cccggatgat aaaatgatct ccgcttttgc taactatgct gtacaaactg aaaattggca
      541 gcaaaatgcg ttacaagcat taacgtctga taaaaaagga ttaactcctg aaaaattatt
      601 agtcttgcag gatcatgtgc ttaattataa tgtcgaggtt tcattggtgg gaacattagc
      661 aagaaaaatt gtcgctgcag ttgagacgtt gacccgctca tgaaatatat atcattatta
      721 ttgtttatcc tgctgttgtg cggctgcaag cagcaggaat tactgaacca tcttgatcaa
      781 cagcaagcta atgatgtcct tgctgtttta caaagacata atattaatgc tgaaaagaag
      841 gatcaaggca aaactggatt ctcaatctat gttgaaccaa cggattttgc ttctgcggtg
      901 gattggctaa aaatctacaa tctcccgggt aagcctgata ttcaaatatc gcagatgttc
      961 cctgcagatg cactcgtttc atctccacga gcggaaaaag caaggctata ttctgctatt
     1021 gagcagcggt tagagcaatc tctcaaaatt atggatggta ttgtttcttc gagagtacat
     1081 gttagttacg atgttgatac tggcgatagt ggtaaaactg ctttaccaat tcatatttct
     1141 gtacttgcag tatatgaaaa agatatcaat ccagaaataa aaataaatga cataaagaga
     1201 tttattgtta atagttttgc aagcgtacaa tatga
//
LOCUS       DAZKIP010000335         1227 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZKIP010000335 DAZKIP010000000
VERSION     DAZKIP010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.339"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..441
                     /locus_tag="ACHOUK_005054"
     CDS             <1..441
                     /locus_tag="ACHOUK_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP9900285.1"
                     /translation="DEKEGNHTTAYRDGAGIWTICRGAILVDGKPVVPGMKLSKEKCD
                     QVNAIERDKALEWVERNIKVPLTEPQKAGIASFCPYNIGPGKCFPSTFYRRINAGDRR
                     GACEAIRWWIKDGGRDCRIRSNNCYGQVSRRDQESALACWGIDR"
     gene            597..779
                     /locus_tag="ACHOUK_005055"
     CDS             597..779
                     /locus_tag="ACHOUK_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900286.1"
                     /translation="MGKYKRILLRLTMKNGLELKAPVTDDVSRALAFAIKWVAVGIAV
                     SPMLYGLAKLVIALKS"
     gene            792..923
                     /locus_tag="ACHOUK_005056"
     CDS             792..923
                     /locus_tag="ACHOUK_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900287.1"
                     /translation="MSDKLITLAKILCVIVGISFSLMLVAICFSTAWRVLTLSWLVG"
     gene            931..1167
                     /locus_tag="ACHOUK_005057"
                     /pseudo
     CDS             931..1167
                     /locus_tag="ACHOUK_005057"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
     gene            1151..>1227
                     /locus_tag="ACHOUK_005058"
     CDS             1151..>1227
                     /locus_tag="ACHOUK_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900288.1"
                     /translation="MRELKMKLCVLMLPLVVSACASTPTV"
BASE COUNT          306 a          258 c          364 g          299 t
ORIGIN      
        1 gacgaaaaag aaggtaacca caccacggca taccgtgatg gtgcgggtat ctggaccatc
       61 tgccgcggtg ccatcctggt ggatggtaaa cctgtcgttc cgggcatgaa gttgtcgaag
      121 gaaaaatgcg accaggttaa cgccattgaa cgtgataagg cgctggagtg ggtggagcgc
      181 aatattaaag taccgctgac cgaaccccag aaagcgggga tcgcgtcatt ctgtccgtac
      241 aacattggtc ccggtaagtg tttcccgtcg acgttttaca gacgaattaa tgcaggtgat
      301 cgcaggggag cgtgtgaggc gattcgctgg tggattaagg acggtggcag agactgccgt
      361 attcgctcaa acaactgcta cggtcaggtt tcacggcgtg accaggagag cgcgctggcg
      421 tgctggggaa ttgacagata agcagaatat tttgctgaaa aatgcggttt gctcacactg
      481 gcggataaca cgaaatcctg cgaactggca aaaactaagt gaataaaagt aaaaccccgt
      541 ttgttggccg caaacggggt tttgtgtttc tgaccttgag taaggcaagg gagaacatgg
      601 gaaagtataa acgaattctg ttgagattga ctatgaaaaa cggccttgaa ctgaaagcgc
      661 ctgtaactga tgacgtcagc agagcgctgg cttttgctat taagtgggtg gcggtcggta
      721 ttgctgtgtc tccgatgctg tatgggctgg caaaactggt cattgcgttg aaatcgtgaa
      781 gaggattaag catgtcagac aagctcataa cgctggcgaa gatcctctgt gtaatcgtcg
      841 gcatttcatt ttcactaatg ctggttgcta tttgcttttc cactgcctgg cgcgtcttaa
      901 ctttgtcatg gctggtgggg tgagggggat atgaaccgtg ttctgtgtgt ggtgattatt
      961 gtcctgctgg tagcctgtgg tgtgcttagt ctggggctga atcattaccg cgataacgcc
     1021 attacctaca aagcgcaacg cgataaaaaa gccagtgagc tgaagctggc gaacgtgaca
     1081 attactgata tgcaggtacg ccagcgtgat gtcgctgcac ttgatgccag atactcgagg
     1141 gaattagccg atgcgagagc tgaaaatgaa actctgcgtg ctgatgttgc cgctggtcgt
     1201 aagcgcctgc gcatcaacgc caactgt
//
LOCUS       DAZKIP010000336         1215 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZKIP010000336 DAZKIP010000000
VERSION     DAZKIP010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.340"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(54..1178)
                     /locus_tag="ACHOUK_005059"
     CDS             complement(54..1178)
                     /locus_tag="ACHOUK_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HFP9900289.1"
                     /translation="MRKRILFIGPPLYGLLYPLISLAQAFRVIGHDVVISSAGKFANK
                     AAEAGLVVFDAVPGLDSEAGYRHQEELRKKSNIIGHFSFFSDEMADNLIDFAGKWRPD
                     LIVYPPLDPAGPLVAAKYRIPSVMLAVGFAHTSAHIQMLNRSLSNAYRRHGVSGPLCD
                     LAWIDVAPPSMSILKNAGEPVISMRYIPYNGGAVKETWWDRDSDRKRLLISLGTVKPM
                     VDGLELISWVMDSANEVDADIILQLAINARTGLRKLPSNVRLVDWIPMGVFLNGADGF
                     IHHGGAGNTLTALYSGIPQIVFGEGADRSVNAEIVAKRGCGIIPDKHGLTSDLVNRLL
                     YDDSLRFCSDQVAAEMAEQPSPAEIAEVLMRKLKNNGKQL"
BASE COUNT          366 a          296 c          216 g          337 t
ORIGIN      
        1 tgaaggacat tatattactc ataacatttc tcgttgaaaa tttcagcaat acgctacaat
       61 tgtttcccgt tgttttttaa ttttctcatc aaaacctctg caatctctgc aggactgggt
      121 tgttcagcca tttcagcggc tacctgatct gaacagaagc gtagtgaatc atcataaaga
      181 aggcgattta ccaaatcact ggtcagtcca tgcttgtccg gaataatccc acacccacgc
      241 ttcgcaacaa tttctgcatt aacagagcga tctgcacctt cgccaaacac aatctgtggt
      301 attccactat acagcgctgt cagggtatta cctgcgccac cgtgatgaat aaatccatca
      361 gctccattaa ggaatacacc cataggtatc cagtcaacca gacgtacatt tgatggtagt
      421 tttcgtaaac cagtacgagc atttattgca agttgcaaaa tgatatcagc atcaacttca
      481 tttgcagaat ccataaccca tgaaatcagc tccagaccat caaccattgg ttttacagtg
      541 ccaaggctga taagtaaacg tttccgatca gaatccctgt cccaccatgt ttcctttaca
      601 gcacctccgt tataaggaat atatctcatt gagataaccg gttctccagc atttttaaga
      661 atgctcatac ttgggggagc aacatcaatc catgctaaat cacatagtgg accgctgact
      721 ccatgccgcc tgtaagcatt gcttaaagaa cggtttaaca tctgaatatg ggcagatgta
      781 tgcgcgaatc caacagccag catcactgaa ggaattctat atttagcagc aaccaatggg
      841 cctgccggat caagaggggg atagactatt aaatctggcc tccattttcc tgcaaaatcg
      901 atgaggttat ctgccatttc atcgctaaaa aaagagaaat gaccaataat attacttttt
      961 ttcctcaact cttcctgatg gcgatatcca gcctctgaat ctaaacctgg aactgcatca
     1021 aaaacaacca gtccagcttc tgctgcttta ttcgcgaatt tgccagcgct actaattact
     1081 acatcatgtc caattacacg aaaggcctga gccagagaaa tcaatgggta taacaaaccg
     1141 tacagcggtg ggccaataaa aagaatacgt tttcgcatta aaaaagtccg tcttctgctt
     1201 acctgtttat ttttc
//
LOCUS       DAZKIP010000337         1206 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZKIP010000337 DAZKIP010000000
VERSION     DAZKIP010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.341"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(88..267)
                     /locus_tag="ACHOUK_005060"
     CDS             complement(88..267)
                     /locus_tag="ACHOUK_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HFP9900290.1"
                     /translation="MINKLLLAYLIGLVVTSTLIFIFSEEKVTYRLFAAIITGLTWPL
                     SLIPSIISLMIRKSD"
     gene            complement(334..>1206)
                     /locus_tag="ACHOUK_005061"
     CDS             complement(334..>1206)
                     /locus_tag="ACHOUK_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900291.1"
                     /translation="ALLIAYLDKANFYVMEDSGAWEEDARLNTSSVALVTSGLERLSN
                     LLSKKDSVFVSDLLREAKVNELDETLSTTRLNHLIDKGYERITLQLDLGGESPGYLEK
                     DKHYREADAALLNVIYPANLSKINTRRKEQVLKIVKKLAGPYGIKRYEKDNYQSANFW
                     FNDIKTDTDQNSHAKREKSFIPSTEAEWFFDSWYAKSAAIVYKESRKEEYLNDSVQFM
                     NRSLAQITGENMIGANGRSVPEMALPESYNYIHKSGTLHEAPSPIIPLNWSKASMTLM
                     LKEMSNLINDEGIK"
BASE COUNT          320 a          249 c          209 g          428 t
ORIGIN      
        1 cacactgcga gtgttttgtt actccccgca tatattttat tctcttgttc gattctattt
       61 ttcttaatcg atcatataat tttcttgtta gtcggattta cgaatcatga gacttataat
      121 ggaaggaatg agactaagcg gccaggttaa acctgtaatt attgcagcaa acaaccgata
      181 tgttactttt tcttccgaga agataaatat taaagtcgat gtcacaacca gacctatcag
      241 gtaagctaac agtaatttat tgatcacttt tacctcccct ttcgatttct ctttgagcgg
      301 tccttttaat ccaggaccgc ctcattatga aaattattta attccctcat cattgataag
      361 gttggacatc tctttcaaca tgagagtcat tgaagctttc gaccaattta gcggaatgat
      421 agggcttggc gcttcatgca aagttcccga cttgtggata tagttatagc tttcgggaag
      481 tgccatttct ggtacgctcc tgccatttgc accaatcata ttctcgcctg ttatttgggc
      541 caatgatcta ttcataaatt gtactgagtc attcaaatac tcttcttttc ttgattcttt
      601 ataaacaatt gctgctgatt ttgcatacca tgagtcaaaa aaccattctg cttctgttga
      661 tggaatgaag ctcttttctc tttttgcatg cgaattttga tcggtatcgg ttttaatatc
      721 attgaaccaa aaatttgccg actgataatt atccttctcg tatcttttta taccatacgg
      781 acctgctagt tttttaacta tttttagaac ttgctctttt cttctggtgt ttatctttga
      841 aagatttgca ggataaataa cattaagtaa agccgcatcg gcttctctgt aatgtttatc
      901 tttttccaga taacctgggg attctccccc taaatcaagc tgtaacgtta ttctttcata
      961 acctttatct atgaggtggt tcaacctcgt tgttgaaaga gtttcatcca gttcattgac
     1021 tttagcttcg cgtagtaaat cagagacaaa aacagagtct ttcttcgaga gtaggtttga
     1081 caaacgttcc agtccagagg tcaccagtgc aacggaagat gtgtttaatc gggcatcttc
     1141 ctcccacgct ccggaatctt ccataacata gaaattggct ttatccagat aagcaatcag
     1201 cagcgc
//
LOCUS       DAZKIP010000338         1201 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZKIP010000338 DAZKIP010000000
VERSION     DAZKIP010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.342"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..1159
                     /gene="ygeH"
                     /locus_tag="ACHOUK_005062"
     CDS             <1..1159
                     /gene="ygeH"
                     /locus_tag="ACHOUK_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HFP9900292.1"
                     /translation="CIYSLRCIFEKIGYDRCIETIYRKGYRFSGQVFKTKINEDNTSD
                     YSIAIFPFTTSLKTLDPLILNQELVQIISNKKIDGLYTYPMAATNFCNDHISQNSFLR
                     RFNPDYFVTGRINQNNAVNTLYIELTDAKNLFLIASNHLPVDELHNTSQFIIENILQT
                     VHNPQRSVRLTKQDQGYKNHYLSDEMLAGKKELYEFTPESIYRAMTIFDGLQNKSDIQ
                     TLKTECYCLLAECHMSLALHGKSELELAAQKALELLDYVSDITTVDGKILAIMGLITG
                     LSGQAKVSHILFEQAKIHSTDIASLYYYRALVNFHNEKIEEARICIDKSLQLEPRRRK
                     AVVIKECVDMYVPNPLKKNMKLYYKETESGSHRVIIDNILKLKQLTRICMR"
BASE COUNT          439 a          211 c          197 g          354 t
ORIGIN      
        1 atgtatctat tctttgcgct gcatttttga aaaaattggc tatgatcgtt gcatagaaac
       61 aatctaccgg aaaggttatc gtttcagtgg gcaggttttc aaaactaaaa taaatgaaga
      121 taacacttca gactattcta tcgctatatt ccctttcact acttcattga aaacactgga
      181 tccattaata cttaatcagg aattagtaca aatcatttca aataaaaaaa tcgatggcct
      241 ctatacctat ccgatggctg cgacaaattt ttgtaatgat cacatatcgc aaaattcatt
      301 tttgagaaga ttcaatccag attatttcgt tacaggaaga ataaaccaga ataatgcagt
      361 gaacacttta tacattgagt tgaccgacgc taaaaacctt ttcctcatcg ccagtaatca
      421 tctacctgtt gatgaactac ataatacatc acaatttatt atagaaaata tccttcaaac
      481 agtacataat ccacaacgat ctgtaagatt aactaagcag gaccaaggat ataagaatca
      541 ttatttatca gatgaaatgt tagccggaaa gaaagaactt tacgagttca cccctgaaag
      601 catttacagg gccatgacta tatttgatgg attacaaaat aaaagtgata tacagacgct
      661 aaaaacagaa tgttattgcc ttctagcgga atgccatatg tctttggcac ttcatggaaa
      721 aagtgaactt gaacttgctg ctcaaaaagc attagagctt ttagattatg tatcagacat
      781 aaccactgtc gatggaaaaa ttttagctat tatgggactg ataactggtc tgtctggaca
      841 agcaaaagta tctcatatct tatttgaaca ggctaagata cactcaactg atatagcctc
      901 tctctactac tatagggcac ttgtcaactt tcataatgaa aaaattgaag aggcaaggat
      961 ttgtatagac aaatcactac aactcgaacc cagaagacga aaagcagttg tgataaaaga
     1021 atgtgtagat atgtatgtgc ctaacccgct caaaaaaaac atgaaactct actataaaga
     1081 aactgagagt ggaagccatc gagttataat tgacaacatt ttgaaattaa agcagctgac
     1141 gagaatttgt atgcgataaa tttttattga cacaatttta ttatttagat gtatgcaact
     1201 t
//
LOCUS       DAZKIP010000339         1199 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZKIP010000339 DAZKIP010000000
VERSION     DAZKIP010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.343"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..372
                     /locus_tag="ACHOUK_005063"
     CDS             <1..372
                     /locus_tag="ACHOUK_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP9900293.1"
                     /translation="QIVLGAAAIVGSFFTAGASMALWGSALAAGGFSATTMLFSLGAS
                     MILGGVAQMLAPKAKVPEYKSTDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRI
                     SQDISTRDEGGDGKVVVIGRG"
     gene            613..>1199
                     /locus_tag="ACHOUK_005064"
     CDS             613..>1199
                     /locus_tag="ACHOUK_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9900294.1"
                     /translation="MGKGGGKAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLLETTSKGDRNHSSVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPPRPFNIRMVRETADSTTDQLQN"
BASE COUNT          292 a          284 c          384 g          239 t
ORIGIN      
        1 cagattgtgc tgggggcggc ggccatcgtg gggtcgttct tcactgccgg ggcatcaatg
       61 gcgttatggg gttcagccct ggcagccggt ggtttttctg ccaccacgat gctgttttca
      121 ctgggggcca gcatgatact gggtggtgtg gctcagatgc tggccccgaa ggctaaagta
      181 ccggagtaca aaagcacgga taacggcaga cagaacacgt acttttcctc gctggataac
      241 atgattgcac aggggaaccc gatgccggtg ccttacggtg aaatgctggt tggctcacgg
      301 cgaatctccc aggacatcag tacccgtgat gaaggcggag acgggaaagt ggtggttatc
      361 gggcggggat gaaaataaaa aaatcccgca gagttagcgg agctgcggga gagaacgatg
      421 aagattaacg ttatggagtt atttttcagg catcaaaaaa gtaatgcagc gtcattattg
      481 cggctacagg caattgccgg aaatgtgaag agtttcagaa attttattcc gtcatgacac
      541 aggcaccctc cggggtgcct gttgttttct ggcataaaca gattcagaca tcagacagga
      601 gagggggaca gagtgggtaa agggggcggc aaggcgcaca cgccggttga ggcaaaggac
      661 aatcttaagt ccacgcagat gatgagcgtg attgatgcga ttggtgaagg gccgattgaa
      721 ggtccggtga aggggctgca gagtatcctg gtgaacaaaa ccccgctgac ggacacggac
      781 ggtaatcctg tgatacatgg tgtgacagcg gtctggcgcg ccggggagca ggagcagaca
      841 ccacctgaag gctttgagtc ctccggagct gaaaccgcac tgggcgtgga agtgacgaag
      901 gcaaagccgg tgacgcgcac cattacgtcc gcgaacattg accgcctgcg ggtcaccttc
      961 ggggtgcagt cactgttgga gaccacctca aagggcgacc gtaatcactc ttctgtccga
     1021 ctgctgattc agttgcagcg taacggtaac tgggtgacgg aaaaggatgt caccattaac
     1081 ggcaagacca cctcgcagtt cctggcgtcg gtgattctgg ataatctgcc gccccggccc
     1141 tttaacatcc ggatggtcag ggagacggcg gacagcacca cggaccagct gcagaacag
//
LOCUS       DAZKIP010000340         1190 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZKIP010000340 DAZKIP010000000
VERSION     DAZKIP010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.344"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            1..1190
                     /locus_tag="ACHOUK_005065"
                     /pseudo
     CDS             1..1190
                     /locus_tag="ACHOUK_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
BASE COUNT          424 a          243 c          221 g          302 t
ORIGIN      
        1 attatatcaa cttttcacgc agaaaaagtc attaatgatc tgttaaaaaa cgattataaa
       61 atttcccttt ctccagatac aacttaccga gagctgaata aagctgcaca gcgtagcatt
      121 acagcgccag acaggatagg agaagggaaa atatgggttt atcaacgaga tacaatggtt
      181 gaaagaggtg ataacagcgg tgttcatcag tatggtccag ctgaacattt cactcacatt
      241 atatctgaca aaccgtcccc aaaagataag tatgttgcat atgctattaa cattcctgac
      301 tatgagctag cagccgatgt atataatatt aacgtgacgt caccttccgg acagcaagaa
      361 acatttaaaa tattaatcaa tccagaacat ctacggcaaa cacttgagcg taaatccctt
      421 actgctgttc agaaatcaca atgtgaaatc atcaccccca aaaaacctgg cgaagcgatt
      481 cttcatgctt ttaatgccac ctaccagcaa atcagggaaa atatgcctga gtttgcacgt
      541 tgccattatg ggtatataca aatccctcca gtgacaactt tccgcgccga cggaccagaa
      601 actcccgaag aagaaaaagg ttactggttt cacgcttatc aacccgaaga tctttgtacc
      661 atccataatc caatgggaga tttgcaggat tttatcgcat tggttaaaga tgctaaaaaa
      721 tttggtatcg atatcattcc tgattatacc tttaacttta tgggaatcgg gggtagtggt
      781 aaaaatgacc tggattatcc ctctgctgat atacgagcga agatcagtaa agatatagaa
      841 ggtggtatcc ctggctattg gcaaggtcag gttttgattc catttactat agatccagta
      901 acaaaagaac gtaaacaaat ccatccagaa gatatacatc tcacagcaaa agacttcgaa
      961 gcaagtaaag ataatatttc taaggatgaa tgggaaaacc tccatgcatt aaaagaaaag
     1021 cgtttaaatg gaatgcctaa aacccccccc aaaagtgact aggttattat gttgcaaaat
     1081 caatacgttc gtgaaatgcg aaaatatggc gtacgaggtt tacgttacga tgcagcaaaa
     1141 cactcaaaac atgaacaaat agaaagatca ataaccccac cgcttaaaaa
//
LOCUS       DAZKIP010000341         1186 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZKIP010000341 DAZKIP010000000
VERSION     DAZKIP010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.345"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..470)
                     /locus_tag="ACHOUK_005066"
     CDS             complement(<1..470)
                     /locus_tag="ACHOUK_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFP9900295.1"
                     /translation="MPLTSDIRSHSFNLGVEVVRARIVANGRGDITVGGETVSIVYDS
                     TNGRFSSSGGNGGLLSELLLLGFNSGPRALGERMLSMLSDSGEAQSQESIQNKISQCK
                     FSVCPERLQCPLEAIQCPITLEQPEKGIFVKNSDGSDVCTLFDAAAFSRLVGEG"
     gene            complement(584..853)
                     /locus_tag="ACHOUK_005067"
     CDS             complement(584..853)
                     /locus_tag="ACHOUK_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFP9900296.1"
                     /translation="MNILKKLMQRLCGCGKHDDREHGELLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            complement(855..>1186)
                     /locus_tag="ACHOUK_005068"
     CDS             complement(855..>1186)
                     /locus_tag="ACHOUK_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFP9900297.1"
                     /translation="GPAGPQGPKGETGATGPVGATGPQGPKGDPGETQIRFRLGPGNI
                     IETNSNGWFPDTDGALITGLTFLAPKDATRVQVFFQHLQVRFGDGPWQDVKGLDEVGS
                     DTGRTGE"
BASE COUNT          304 a          351 c          230 g          301 t
ORIGIN      
        1 aagccttcac caaccaaacg agaaaatgca gcggcatcaa ataaagtaca tacatctgaa
       61 ccatctgaat tcttcacaaa aatacctttt tcaggctgct ccagtgtaat tggacactga
      121 atagcctcaa gcgggcactg aagtctctct ggacaaacag aaaacttaca ttgagatatt
      181 ttgttctgaa tactctcttg cgattgtgct tcacctgagt ccgaaagcat acttagcatt
      241 ctctcaccaa gggctcgagg accactatta aatcccaaaa gcaataactc agaaagcaat
      301 ccgccattac cgccactgga tgaaaagcgc ccattagtag aatcatacac aatactgaca
      361 gtttcaccac cgactgtaat atctccgcgc ccattggcta caattcgggc acgaacaacc
      421 tccaccccaa gattaaatga atgtgatcta atatctgagg ttaatggcat aacaaagctc
      481 cctattttaa tttgaactcc agacttaaat agctgtaaca aacatccccc atacgtgaca
      541 acaaaaaacc ggagccggac tccggttttt gtgaagctgt cggctatttc atcccgccaa
      601 tattttccca cgtcccgtca gcacgcagga tttgcagcgg tcttaccaca cactgtatct
      661 gctttttatc cgcatccagt atcaccacct gcgtgattac cctgtcctgc tccgggataa
      721 taccattctc atctgactcc aggatgtctg ccggccccag tcgcagctgt gctgtaagta
      781 actccccgtg ttcacggtca tcatgctttc cgcaaccaca cagacgctgc ataagttttt
      841 ttagtatgtt catgtcattc tcctgttctg cctgtatcac tgcccacttc atccagcccc
      901 ttaacatcct gccacggccc gtcaccaaac ctgacctgca aatgctgaaa aaaaacctga
      961 acccgtgtgg catctttggg ggcaagaaag gtcagtccgg tgatgagtgc gccatctgta
     1021 tccgggaacc agccattgct gtttgtctca ataatgtttc ccggccccag acgaaaacgg
     1081 atttgtgtct cccccgggtc gcccttcggt ccctgaggtc cggttgcccc caccgggcca
     1141 gtcgcacctg tttctccttt cggtccctgt gggcctgccg ggcctg
//
LOCUS       DAZKIP010000342         1174 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZKIP010000342 DAZKIP010000000
VERSION     DAZKIP010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.346"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            548..838
                     /locus_tag="ACHOUK_005069"
     CDS             548..838
                     /locus_tag="ACHOUK_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFP9900298.1"
                     /translation="MNLSASMNDAGDGFELLMSGNMQDFNICCRRDNHYPEHKLIPLS
                     ELFDVNYGLNLELNKLEKDSSGINFVSRTSKNNGVSARVKLKDGISPCQQLC"
     gene            862..1113
                     /locus_tag="ACHOUK_005070"
     CDS             862..1113
                     /locus_tag="ACHOUK_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFP9900299.1"
                     /translation="MESFVQDSPFYSGRDLYWLRPKVELTLEEKLYYCSCIRRNRHKY
                     SYGRQANRTLKNLLVPSLDSVPAWVYGVTGKIISELSER"
BASE COUNT          325 a          212 c          282 g          355 t
ORIGIN      
        1 ttgaaatagt atgtttctca tcgaaagcaa gtttgtcaat ataaatttgt aacagaaatg
       61 actttccggg gataaacaaa actgttcatg gcaatggctt tctctgtcgc caacatggat
      121 acattgttcc ggataaagca ctgatgtcat tgactgacgc agactatcag gccatctgat
      181 gctttctttt ccttcaccgg tattgatgat atgccttatg aagggggact tattcctgta
      241 acccaatcac aggcactgtg attactggtt tatgattgat gctgatttga gcagatatgc
      301 acagcagttg tggattcagg ggatattttc tgccagatat tcataccaga ttgatgacct
      361 ctttgctcag atgctcttcg ttctgagcca gccaggattt tgtggccaca tacattgttc
      421 gccgatgggg aatcgagaca ggcgattggc tgtgatgcga ttttgccgct aactgaggat
      481 gagtccgccc ctcccgggga attcctgtaa gttaagccgt acctgttgat tagcactaaa
      541 ctgggtaatg aatttatctg ccagtatgaa tgatgccggg gacggttttg aactcctgat
      601 gtcgggtaat atgcaggatt ttaatatttg ttgtcgtcgg gataatcatt atcctgaaca
      661 taaactgata cctttatcag agttatttga cgttaattat ggactgaatc ttgagctgaa
      721 taaactggag aaagattctt caggaattaa ttttgtttca agaacgtcca aaaataatgg
      781 cgtctccgcc agggttaaat taaaagatgg tattagtcca tgccagcagc tgtgttaact
      841 gtagcagcag gaggttctgt tatggaatcg tttgtacagg attcaccatt ttatagtgga
      901 agagatcttt attggttacg tcctaaagtt gaactgactc tggaagagaa attgtactac
      961 tgctcttgta taagaagaaa cagacacaaa tacagctacg ggcgtcaggc aaaccgaacg
     1021 ctgaagaatt tactggtacc gtcattagat tctgttccgg catgggtata tggtgtgaca
     1081 gggaaaataa tatctgaatt gtctgaacgt tgagactaag agggccggag gccaggtgaa
     1141 tggccaccgg gatatttata tcagagtata ttgg
//
LOCUS       DAZKIP010000343         1172 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZKIP010000343 DAZKIP010000000
VERSION     DAZKIP010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.347"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            32..>1172
                     /locus_tag="ACHOUK_005071"
     CDS             32..>1172
                     /locus_tag="ACHOUK_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9900300.1"
                     /translation="MKKAIAYMRFSSPGQMSGDSLNRQRRLIAEWLKVNSDYYLDTIT
                     YEDLGLSAFKGKHAQSGAFSEFLDAIEHGYILPGTTLLVESLDRLSREKVGEAIERLK
                     LILNHGIDVITLCDNTVYNIDSLNEPYSLIKAILIAQRANEESEIKSSRVKLSWKKKR
                     QDALESGTIMTASCPRWLSLDDKRTAFVPDPDRVKTIELIFKLRMERRSLNAIAKYLN
                     DHAVKNFSGKESAWGPSVIEKLLANKALIGICVPSYRARGKGISEIAGYYPRVISDDL
                     FYAVQEIRLAPFGISNSSKNPMLINLLRTVMKCEACGNTMIVHAVSGSLHGYYVCPMR
                     RLHRCDRPSIKRDLVDYNIINELLFNCSKIQPVENKKDANETLELK"
BASE COUNT          401 a          173 c          257 g          341 t
ORIGIN      
        1 tttataaaag cataagttag caggtgtcac catgaaaaaa gccatagcat atatgcgatt
       61 ttcatcacca ggtcagatgt ctggcgactc attaaaccga cagagaagac ttattgctga
      121 atggttaaag gtaaatagtg attattatct tgataccata acatatgaag atttaggatt
      181 aagtgcattc aaaggaaagc atgcacaatc aggagctttt tcggaatttt tagatgctat
      241 agagcatggt tatatattgc caggaactac attgttagtt gaaagtctgg acagactttc
      301 aagagaaaaa gtcggtgaag cgattgaacg tctgaaattg attttgaatc acggtattga
      361 tgttataact ctttgcgaca atacagtcta taatattgac tctttgaatg agccatattc
      421 attaataaaa gccatactta tagcacaaag ggcaaatgaa gaaagcgaga taaagtcaag
      481 tcgggttaaa ttatcatgga agaaaaaacg gcaggatgca ctggaatcag gtacgattat
      541 gacggcgtct tgtccgagat ggctctcctt agatgacaaa agaacggctt ttgttccaga
      601 ccccgacagg gtgaaaacta ttgagctaat ttttaaactc aggatggaaa ggcgctcatt
      661 gaatgcaata gccaagtatt taaatgatca tgctgtaaag aatttctcag gaaaagaaag
      721 tgcatggggg ccttctgtaa ttgaaaaatt attagcgaat aaagctctga taggtatatg
      781 cgtaccttca tatcgtgcaa gagggaaagg gataagtgaa atcgctggct attatcccag
      841 agtcatatca gatgatttgt tttacgctgt acaggaaatt cggttggcac cttttggtat
      901 tagcaatagt agcaagaatc ctatgctaat aaatctactt cgaacagtta tgaagtgtga
      961 ggcttgtggt aataccatga ttgttcatgc ggtatctgga agtttgcatg gctattatgt
     1021 ttgtccgatg agaagattac atcgatgtga caggccatca ataaagagag atttggttga
     1081 ttataatatc attaatgaat tgctttttaa ttgtagcaaa attcaaccag ttgaaaacaa
     1141 gaaagatgct aatgaaactt tagagttaaa aa
//
LOCUS       DAZKIP010000344         1153 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZKIP010000344 DAZKIP010000000
VERSION     DAZKIP010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.348"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>1153
                     /locus_tag="ACHOUK_005072"
     CDS             <1..>1153
                     /locus_tag="ACHOUK_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP9900301.1"
                     /translation="RRTGKRVWRRGRDLTGWMSLSRKPEMTWYGWDGDRLTTVQTDTT
                     RIQTVYEPGSFAPLIRIETDNGEREKAQRRSLAEKLQQEGSEDGHGVVFPAELVRLLD
                     RLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEPEYTPARKVHLYHCDHRGLPLALI
                     SEDGNTAWSGEYDEWGNQLNEENPHHLHQPYRLPGQQYDKESGLYYNRNRYYDPLQGR
                     YITQDPIGLEGGWSLYAYPLNPVNGIDPLGLSPADVALIRRKDQLNHQRAWDILSDTY
                     EDMKRLNLGGTDQFFHCMAFCRVSKLNDAGVSRSAKGLGYEKEIRDYGLNLFGMYGRK
                     VKLSHSEMIEDNKKDLAVNDHGLTCPSTTDCSDRCSDYINPEHKETIKAL"
BASE COUNT          315 a          243 c          372 g          223 t
ORIGIN      
        1 cgccggacgg ggaaacgggt gtggcgtcgc gggcgtgacc tgacgggatg gatgtcgctg
       61 tcgcggaaac cggagatgac gtggtacggg tgggacggcg accggctgac gacggtacag
      121 accgacacca cgcgtatcca gacggtatac gagccgggaa gcttcgcgcc gctcatccgc
      181 atcgaaacag acaacggtga gcgggagaaa gcgcagcgcc gcagcctggc ggagaagctg
      241 cagcaggaag ggagcgagga cgggcacggt gtggtatttc cggctgaact ggtgcggctg
      301 ctggacagac tggaggaaga aatccgggca gaccgcgtga gcagtgaaag ccgggcgtgg
      361 cttgcgcagt gcgggctgac ggtggagcaa ctggccagac aggtggagcc ggaatacaca
      421 ccggcgagaa aagttcatct ttaccactgc gaccaccggg gcctgccgct ggcgctcatc
      481 agcgaagacg gcaatacggc gtggagcggg gagtatgatg aatggggcaa ccagctgaat
      541 gaggagaacc cgcatcacct gcaccagccg taccggctgc cggggcagca gtatgataag
      601 gagtcggggc tgtactacaa ccggaaccgg tactacgatc cgttgcaggg gcggtatatc
      661 actcaggacc cgatagggct ggagggggga tggagtctgt atgcgtatcc gctgaatccg
      721 gtgaatggta ttgatccatt agggttaagt cccgcagatg tagcgctaat aagaagaaaa
      781 gatcaactaa accatcaaag agcatgggat atattatctg atacttatga agatatgaag
      841 agattaaatt taggtgggac tgatcaattt ttccattgta tggcattttg tcgagtgtct
      901 aaattaaatg acgctggtgt tagccgatcg gcgaaagggc tgggttatga aaaagagatt
      961 agagattacg ggttaaatct gttcggtatg tacggcagaa aagtaaagct atcccattct
     1021 gaaatgattg aagataataa aaaagacttg gctgtaaatg accatgggtt gacatgtcca
     1081 tcaacaacag attgctcaga tagatgtagt gattatatta atccagagca taaagaaacg
     1141 ataaaggctt tac
//
LOCUS       DAZKIP010000345         1127 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZKIP010000345 DAZKIP010000000
VERSION     DAZKIP010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.349"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            199..>1127
                     /locus_tag="ACHOUK_005073"
     CDS             199..>1127
                     /locus_tag="ACHOUK_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900302.1"
                     /translation="MSPDNIGSAVNRFSGWPSNDSLKTDKESMTSPVTVQVIKMIAAI
                     LDPHRIVVGIDTLYTTQRETFTSESQQLIESALQTLHVMNLNNLETNRDEESHYLAFE
                     YNVDTSDRITVSAYNEKKPGCYGQCLVIQDDETDLFNLIKIKIFEARHNYVAQLMSDP
                     EFMFKFSYSAQQNRLEPHLLVPAYFPLKTNEPVTQEDVLLLYRFFKMNDDFNKLTSDE
                     YMSILTPLMKCERSVHDNNRYVTGKDTLLLDYPPSGNQIHFHVFPDESATLVLYFSNN
                     DIECFVFERDIPSQYRFFKMFTNLALVIDQLTEA"
BASE COUNT          351 a          229 c          210 g          337 t
ORIGIN      
        1 cccgctatgt tattgtttca ttagtaataa tcacttaaat gtatcgatga aacaggtatg
       61 acttaccggg agtgattgtt tctgagtgaa cagaggccac tttcgcctga taaatgaaaa
      121 atgagcgatt tttgcaagtt agtttttcta tagccgagta cgctcagcgc ctcgcttgat
      181 tacaatgagg ttgctgctat gagtcctgat aatatcggtt cggctgtcaa tcgcttctca
      241 ggttggccat ctaatgattc tctaaaaaca gataaagaat caatgacatc acccgtgact
      301 gtgcaggtaa taaagatgat tgccgccatc cttgacccac atcggatagt cgttggaatt
      361 gatacacttt atacaacgca gcgcgaaaca tttacctcag agagtcaaca gctcattgaa
      421 tcagcattgc aaacacttca tgtgatgaat ttaaataatc tggaaacaaa tcgcgatgaa
      481 gaaagtcact atctggcttt tgaatataac gttgacacct ctgacagaat aaccgtttct
      541 gcttataatg aaaaaaaacc tggctgctat ggccaatgtc ttgtaataca ggatgacgaa
      601 acagacttat ttaacctgat aaaaattaaa attttcgaag ccaggcataa ttatgtcgct
      661 caattgatga gcgatccgga atttatgttc aaattcagtt atagcgctca acaaaataga
      721 ttagaacctc atcttctggt tcccgcttat ttccctctaa aaacaaacga accggttacg
      781 caagaggatg tactgttact ttatcgtttc tttaaaatga atgatgattt taataaatta
      841 acatcagatg aatacatgag tatcctgaca cctctgatga agtgtgagcg tagcgtccac
      901 gacaacaaca ggtatgtgac gggcaaagat acccttcttc tggactatcc tccttctggt
      961 aaccagatac atttccatgt tttccctgac gaatcagcaa cactagtcct ttatttttca
     1021 aacaatgaca ttgaatgttt cgtctttgaa agagatattc cgagccagta tcgctttttc
     1081 aaaatgttta ccaatctggc actggttata gatcagttaa ccgaagc
//
LOCUS       DAZKIP010000346         1045 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZKIP010000346 DAZKIP010000000
VERSION     DAZKIP010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.350"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            4..>1045
                     /locus_tag="ACHOUK_005074"
     CDS             4..>1045
                     /locus_tag="ACHOUK_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFP9900303.1"
                     /translation="MVDNVTVSRVCIQSPSFVPDLDGEKNKSQLFVDDIVAYLKSPSV
                     YSLEKEGPLNHFVNHCSEVELGFYSDGAYSILVSRSKQQPEGMILTVSDADAINIVHI
                     SVSPVLIKFLDDIFTCLHTYPDDESFTKEQIKANSKYDIVDYNCLLHFTGKPKSLIEC
                     RHFALQYCIDSMNEHTGKVPLKAYYSSPEDIQKHIPFELEQQFNNLQKNPPPGTCVVA
                     SDKFGEALSVFFHRMEKEKLTHMTAIVQSQTHAMAVRLRIKKTPAGETEYVVSFYDPN
                     ATNTAVRYKANNCDSFGSLQSFINIQQAKQKWVITDICSECVGITPYLPREQAHLLSG
                     IENELQPPLSPPA"
BASE COUNT          345 a          191 c          191 g          318 t
ORIGIN      
        1 aaaatggttg ataacgtaac tgttagcaga gtatgtatac aatctccttc tttcgtgcct
       61 gatttggatg gagaaaaaaa taaatctcaa ttattcgttg acgatatagt tgcgtatctt
      121 aaaagtcctt cagtttattc acttgaaaaa gaggggccgt taaatcattt tgttaatcat
      181 tgttctgaag ttgagttagg tttctatagc gatggtgcat attcaattct tgtctccaga
      241 tctaagcaac aacctgaagg tatgatttta accgtaagcg atgcagacgc aatcaatata
      301 gtacatattt ccgtatctcc agtgcttata aaattcctgg atgatatttt tacttgcctt
      361 catacgtacc ctgatgatga gagttttaca aaagagcaga taaaagctaa tagcaaatat
      421 gatattgtag attataattg cctgttgcat tttactggaa aaccaaaaag tttaatagaa
      481 tgtagacatt ttgctctgca atactgtata gattcaatga atgagcatac agggaaagtt
      541 ccattaaagg cttactattc atctccggaa gatatacaaa aacatattcc tttcgagctt
      601 gagcagcaat ttaacaatct acaaaaaaat ccaccacccg gtacatgcgt cgttgccagt
      661 gataagtttg gggaggcatt atctgtcttt tttcacagaa tggaaaaaga gaagttaacg
      721 catatgacgg caatcgttca atctcaaaca cacgctatgg ccgtccgctt gaggatcaaa
      781 aaaacgcctg ctggtgaaac agaatatgtt gtatcctttt atgatcctaa tgcaaccaat
      841 actgcagtac gctataaagc aaacaactgt gattcttttg ggtcattgca atcgtttata
      901 aatattcaac aggcaaaaca aaaatgggta ataacagata tttgctccga gtgtgtagga
      961 ataacccctt atctccctcg ggaacaagcc catttattaa gtggtattga aaatgagttg
     1021 caacctccat tatcaccacc agcat
//
LOCUS       DAZKIP010000347         1045 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZKIP010000347 DAZKIP010000000
VERSION     DAZKIP010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.351"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..842
                     /gene="yeeO"
                     /locus_tag="ACHOUK_005075"
     CDS             <1..842
                     /gene="yeeO"
                     /locus_tag="ACHOUK_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFP9900304.1"
                     /translation="SWPGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSY
                     FKPLNFSIIWEVMGIGIPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALI
                     NLPGSALGSASTIITGRRLGVGQIAQAEIQLRHVFWLTTLGLTAIAWLTAPFAGVMAS
                     FYTQDPQVKHVVVILIWLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRV
                     VVGYVLGIMLGWGVVGVWMGMFADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEKKPA
                     VSE"
     gene            943..1018
                     /locus_tag="ACHOUK_005076"
     tRNA            943..1018
                     /locus_tag="ACHOUK_005076"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:976..978,aa:Asn,seq:gtt)
BASE COUNT          212 a          206 c          301 g          326 t
ORIGIN      
        1 tctcctggcc gggactggga tttgtcgggg ctgggttggg tttaaccatt tctcgttata
       61 ttggcgcagt tgcaattttg tgggtgctgg cgattggttt taatcctgcg ctaaggattt
      121 cgttaaagag ctattttaaa ccgctgaatt ttagcattat ctgggaagtc atggggattg
      181 gtattccagc gagtgtcgaa tcagtgttat ttaccagtgg tcggttatta acccaaatgt
      241 tcgttgccgg aatggggacc agtgttattg ccggaaattt tatcgcgttt tcaattgcgg
      301 ctcttatcaa cttacccgga agtgcgctcg gttctgcttc tacgatcatt acaggccgaa
      361 ggttgggggt agggcagata gcgcaagcag agattcagtt gcggcatgtg ttttggctga
      421 ccactcttgg attaacggcc atcgcctggc taacggctcc ctttgccggg gttatggcat
      481 cgttttacac ccaggatcca caggttaaac atgtcgttgt gattctgatt tggctaaatg
      541 ctttatttat gcctatttgg tccgcttcat gggtgctacc cgctggattt aagggggctc
      601 gtgacgcccg ttacgctatg tgggtttcta tgttgagcat gtggggttgt cgggttgtcg
      661 tcggttatgt gctgggcatc atgcttggct ggggtgtggt tggtgtctgg atgggaatgt
      721 ttgccgactg ggctgtgcgg gccgtgctgt tttactggcg aatggttact ggacgttggc
      781 tgtggaaata ccctcgaccc gaaccgcaaa agtgtgaaaa aaagccagct gtgtcggaat
      841 aaacgacaaa atgcagatta tttcagcaaa cgatttcaaa tttaaaaaac aggctttgac
      901 attgtgggtg ggcatcgcta atattcgcct cgttctcacg attcctctgt agttcagtcg
      961 gtagaacggc ggactgttaa tccgtatgtc actggttcga gtccagtcag aggagccaaa
     1021 ttttagtttt gaggcatctt tatga
//
LOCUS       DAZKIP010000348         1039 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZKIP010000348 DAZKIP010000000
VERSION     DAZKIP010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.352"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..356)
                     /locus_tag="ACHOUK_005077"
     CDS             complement(<1..356)
                     /locus_tag="ACHOUK_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP9900305.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTAQGGERYFFCNELNE
                     KGEAVTWQGRQYQAYPIDGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFV"
     gene            complement(356..685)
                     /locus_tag="ACHOUK_005078"
     CDS             complement(356..685)
                     /locus_tag="ACHOUK_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9900306.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            complement(682..>1039)
                     /locus_tag="ACHOUK_005079"
     CDS             complement(682..>1039)
                     /locus_tag="ACHOUK_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9900307.1"
                     /translation="GGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYA
                     EGGYVGGAGSPAQMRRAEGINFNQNNHVVIQNDGTNGQAGPQLMKAVYDMARKGAQDE
                     LRLQLRDGGMLSGSGR"
BASE COUNT          214 a          388 c          218 g          219 t
ORIGIN      
        1 acaaaattca ccgcatccag aaaacgggca tacacccggc ggcggaccac cgtggccccc
       61 accaggctct gcaggtcctc cgccatcccg gtgaccagac caaacagatt cgacaccgtc
      121 agcgacggtc tggcactgct gcccttcccg ttcatctcaa agccactgcc gtcaatcggg
      181 tatgcctgat attgccgccc ctgccaggta accgcctccc ctttttcatt cagctcattg
      241 cagaaaaaat accgctcacc gccctgcgcc gtcaggtcga tttcccagag taccacccgc
      301 ggtgactgct ctgatttaac cgactcgttc agactttctt cgtgaatatc ctgcatcagt
      361 tcaccacctg cttaaactcc gcgctgaact caacgcgcaa catcccgacc cgcgcagacc
      421 acccggcaca ggtcaccttt atctgccggt atgcataggg tggcttccac aaaaatgcct
      481 tccagcctcc gtgctctgcc aggaacgctt ccagatgccg ggcctcctcc cgggtcacgg
      541 aaagcgtcac acggtatgtt ttcaggtcag cattcagccc tgccgccata cgctgtgagt
      601 acccgtcacc aaaacgcact tcacgcaccg atggctgcga gttcacctcc atatccggct
      661 tcactttcca gcgaaaggtt ttcatcgccc gctccccgat aacataccgc catcacgcaa
      721 ctgcagccgg agctcatcct gcgccccctt gcgggccatg tcatacaccg ccttcatcag
      781 ctgcggcccc gcctgtccgt tggtgccgtc gttctgaatc accacgtgat tgttctgatt
      841 aaaattaata ccttccgccc gccgcatctg cgccggactt ccggcaccac ccacataacc
      901 accttccgca tagccgcgca tcagacggta aagattcccc acacctatcc ggctggttgc
      961 ctctttcgtg aaaacaaact ccccgcggtg aactatcccc gcaggctcat atttgccgcc
     1021 cgtccccgta aatcctccg
//
LOCUS       DAZKIP010000349         1035 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZKIP010000349 DAZKIP010000000
VERSION     DAZKIP010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.353"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..730
                     /locus_tag="ACHOUK_005080"
     CDS             <1..730
                     /locus_tag="ACHOUK_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4225 domain-containing protein"
                     /protein_id="HFP9900308.1"
                     /translation="INEKARLINELKMESETARKEYQLFRQGNYDKYITTDIFEEHGL
                     IKYVNLSFDIVASVGQVVGGVGALKFGKVVHSNRIKGIGVTLVAHGANNFYESLSPLF
                     FNEYDSGPIRELYRVIAKKMGGDINSADYAYSIVDFSITAYGGYSGMKIVPKYNRLIR
                     PSLGNRPGTGRLFHYTSVDFKNKFSLKPTPLKIIQISSTVKKFKVTFYDENTNLRIKH
                     GLNNHMPHQLLMNISGTTTGAGIQ"
BASE COUNT          391 a          159 c          174 g          311 t
ORIGIN      
        1 catcaatgaa aaagcacgac ttatcaatga attaaaaatg gagtcagaaa cagcaagaaa
       61 agaatatcaa cttttcagac aaggaaatta tgataaatat atcactactg atattttcga
      121 agaacatggt ttgataaaat atgtgaactt aagtttcgat attgttgcat ctgtaggaca
      181 ggtcgtaggc ggggttggcg cgttgaaatt tggtaaggta gtgcattcaa accgaataaa
      241 aggtataggc gtcacattag ttgctcatgg ggcgaataat ttttatgaaa gcttatcacc
      301 tttatttttt aacgagtatg attcaggtcc aatacgtgaa ctctacagag taatagcaaa
      361 aaaaatggga ggtgatatta attctgctga ttatgcatac agcatcgtgg acttttcgat
      421 tactgcatat ggtggatatt caggcatgaa aatcgtacct aagtataata gattgattcg
      481 tccttcatta ggaaatagac ctggtacagg acgattattt cattacacat cagttgattt
      541 taaaaataaa ttctcattaa aacctacccc tttaaaaatc atacaaatta gttctactgt
      601 aaaaaaattc aaggttacat tttatgatga aaatacaaat ttgaggataa aacatggcct
      661 caataatcat atgccacatc aattattaat gaatatatca ggaactacaa ctggtgcagg
      721 aatacaataa cagaataact taattttaag ataaaacatt ttgaggcata attggtaaga
      781 gaatttagta tccagttttc tgtgtatgat aagaatagcg aacatggcaa tactaacgat
      841 tgaagacaga aaaaaacttt tcaacatatt atcctgaaaa aatgagaata caagcaatat
      901 cccattaact atacttacca acccaaaccc caaagagcaa taacccacca ttgaaataat
      961 agcatcaatg aaattttgtt ctacgagaga gacttcatgc ttattagcaa gtttcttcat
     1021 ttctttgtat cttga
//
LOCUS       DAZKIP010000350         1035 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZKIP010000350 DAZKIP010000000
VERSION     DAZKIP010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.354"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..721)
                     /locus_tag="ACHOUK_005081"
     CDS             complement(<1..721)
                     /locus_tag="ACHOUK_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP9900309.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGICNDL"
     gene            complement(727..>1035)
                     /locus_tag="ACHOUK_005082"
     CDS             complement(727..>1035)
                     /locus_tag="ACHOUK_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP9900310.1"
                     /translation="VISRWRIEQCSELSAVSASFVLSTPTETDGAVFPGRIMLANTCT
                     WTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNVGNFGGFLSINKLSQ
                     "
BASE COUNT          230 a          329 c          271 g          205 t
ORIGIN      
        1 ccaaatcgtt gcaaatcccc gtaaaggcag atgcgcgcca tgcccggtga cgccagaggg
       61 agtgtgtgcg tcgctgccat ttgtcggtat acctctctcg tttgctcagt tgttcaggaa
      121 tatggtgcag cagctcgcca tcaccacagt aaatggcggc atgattcggc accgatgaac
      181 caaaacagca cagcagcaca tcgcccggtt gtgctgatga caacggcacc tgatacagcc
      241 ctgtggcctc cagattatcc agatagagat tctgaccgtg acgccaccag tcatcctcgc
      301 gatgaaaatc cggcatctca atccccgcca gatgataagc atcccggaac agcgtgtaac
      361 agtccgtcac cccgtgctca aagcgccgcc cggtgagatg cggcacacag cggaacttat
      421 gaatcgtccc ccggcagacc agccaccacg gcaaatcact ctgcacctgc agccgccggt
      481 cggcctcact cagccagggc agaccaccgg ggtggctgtg gaccagcgcc acaatctcac
      541 cctgcatttc tgcctgcagc cagtcttccg gcgacatacg gaaatacgcc tccggctcac
      601 cggagatatt cacgcagggg aaatatcttt ccccctccgg cgtgcttacc acgaagccgc
      661 acgactccgc tggcgcacat cgccgggcgt gcgccagaat cgctgattct gtctgtgtca
      721 tgggatttac tgcgaaagtt tgttaatgga aaggaagccg ccaaagttgc cgacgttatt
      781 gcggaactta caaccgctca ggcatttgct gcatttatcc ttcgtgatat cggacgttgg
      841 ctggtcatat tcatccgcga ccgccggacc gtgataaccg cactcatcgc cgcgataggt
      901 ccaggtgcag gtgttggcca gcatgatacg tcccggaaaa acagcgccgt ccgtttccgt
      961 cggcgtggac agtacaaaag aggcactcac cgcgctcagt tcgctgcact gctcaatgcg
     1021 ccagcggctg atcac
//
LOCUS       DAZKIP010000351         1029 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZKIP010000351 DAZKIP010000000
VERSION     DAZKIP010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.355"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(1..138)
                     /locus_tag="ACHOUK_005083"
                     /pseudo
     CDS             complement(1..138)
                     /locus_tag="ACHOUK_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            288..956
                     /locus_tag="ACHOUK_005084"
     CDS             288..956
                     /locus_tag="ACHOUK_005084"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9900311.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
BASE COUNT          302 a          228 c          200 g          299 t
ORIGIN      
        1 agctactgat gtatcaacac gattcaacaa tacccgatat ttattccatg cctccagcaa
       61 cgatctttct tcctccgttg cgatttccag atctacagca tcctgcagtg gcgcaatata
      121 ctcactgaat tcctggatgt agaactgtgt ggtgacggtc ttccagccat tcggctcctg
      181 ctgtatcgca gcataccagg ctatttcaat atcgctatgc tgcggcagca tttaacccct
      241 tgtaattcat cgccataatt gatttaattc acaaataaaa ctataacatg gtgaaattaa
      301 tgaaaaaaaa cacagatgat ggggctaaaa tttacacacc acttacccta aagctttatg
      361 actggtgggt tttgggagta tcaaatcgtc ttgcatgggg atgtcctaca aaggaacacc
      421 ttcttccaca ctttctggaa catttaggta acaaccatct ggatattggt gttggaactg
      481 ggttttacct tactcacgta cctgagagta gtctgatatc tttaatggat ttgaacgaag
      541 ctagcctgaa cgcggcatct acaagggctg gggaatcaaa aattaaacat aaaattagcc
      601 atgatgtttt tgaaccttat cccgcggcgt tacatggtca atttgattcc atttccatgt
      661 tttaccttct tcactgcctg cctggaaata tatctacaaa aagctgtgta atacgcaatg
      721 cggcgcaggc cttaactgac gatggaactc tatacggagc cacaattctt ggcgatggag
      781 ttgtgcacaa tagcttcggt caaaaactga tgcgcattta caatcagaaa ggcatctttt
      841 caaacacaaa agattccgaa gaaggcttaa cacatatact ctcagagcat ttcgagaatg
      901 ttaaaaccaa ggttcaaggt actgtagtaa tgttttccgc ttcagggaaa aaatagcatc
      961 caaccgcagc acgttcttgc atacgccgtg tctgcggttt aatttttact ctcaaatatt
     1021 gtagttaag
//
LOCUS       DAZKIP010000352         1009 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZKIP010000352 DAZKIP010000000
VERSION     DAZKIP010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.356"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            253..328
                     /locus_tag="ACHOUK_005085"
     tRNA            253..328
                     /locus_tag="ACHOUK_005085"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:286..288,aa:Ile,seq:cat)
BASE COUNT          309 a          176 c          211 g          313 t
ORIGIN      
        1 ataaacttat attattagat acaaaatcat tacaaagtat cattgaatcg atgataacta
       61 tagattgtgc taattattaa catttgcttg ttgactgcga gtatcgactt tgcggaatgg
      121 aggtggtgtc gggttttatg tcgcatgtat tgacgaccca ggatggatga gaacgcataa
      181 aaatctgtga tttacatcat aagatcatca tgtttattgc gtgggttata agtattgtta
      241 ttgcatactt aaggcccttt agctcagtgg ttagagcagg cgactcataa tcgtccggtc
      301 gctggttcaa gtccagcaag ggccaccaaa tcatcattca tgtatcagga agagttaagg
      361 gtacaagggt tactcgatgg cggtctatat ttcacgatta acgccatttc aaggaagaag
      421 tgcggtgggt ggtttcgaat gagggctggt gttttggtac gtggcacgcc actgcaatgt
      481 atttatctat cagtactcat tcaaaagtat ttggaaaaaa tgcctgctat gccaagcggg
      541 caaattgctt acatgtggaa ttaagtaatt aacgataaag ccagcgtatt atcatttatt
      601 atacccattc atttaatcag gttaattact caaatcactg aactattctc tgttcgctca
      661 ctgatgataa gggagtacca caaccaatgt atgtagaaca atgccgtaga gtaaggatga
      721 aatgtctctg ccacgcctta aacatctact actgactaga acacccgccg caggtagatg
      781 agttttgtga gcgttacagg agcgtggcta tgctcattaa tcaacgctat ttagatataa
      841 atttctaaca ctcgaaaaac gattttcact aataaattat aacgggtggt ttttatcata
      901 cagcgaaata accttttgtt tattaaattt aacattcctg tgctaattta attaaaagtt
      961 ccatgcgtag caagtgaagc agttaatgtc agcaattgtc tcaaatgag
//
LOCUS       DAZKIP010000353         1009 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZKIP010000353 DAZKIP010000000
VERSION     DAZKIP010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.357"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>1009
                     /locus_tag="ACHOUK_005086"
     CDS             <1..>1009
                     /locus_tag="ACHOUK_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900312.1"
                     /translation="SYALIDNASLTAVERTLGDILVKNPDTINGDLVAFENLIQAILF
                     YDTLICVDNYKKEYRDKRIAKFDFIKFVSESDFQLSELDQLAQVESRQITSEIRGGEF
                     VDDDFRQLVEMLKLNMICTWDLRSSVYYLTMKMLGQPGTPEYAKYSELSASIFNELSD
                     ASDTKGYWSTDVKLVSSSGHEYTEREFELEREKSTRGKGGMTRSLEMFIASLNWLAYK
                     SIYYSVIAKHFKADSFIHPIRHAYQLHWMKKTGAFGHDYTARLVQSLSDKISTARSEI
                     VDHGRTSTISLDLPIFSAWLANETGNVSQVINSALELRTHDHFRTCRDVIREIHVTYD
                     ES"
BASE COUNT          319 a          159 c          208 g          323 t
ORIGIN      
        1 gtcttatgcg ttgattgata atgcttcgtt aacagcagtc gaaagaacac tgggtgatat
       61 tcttgttaag aatccagata ctattaacgg agatttagtc gcttttgaaa atcttattca
      121 agcgatactt ttttacgata ccctgatctg tgtagataat tataaaaaag aatatagaga
      181 taaacgcatt gctaaatttg actttattaa atttgtatct gaaagtgatt ttcaactcag
      241 tgaacttgat cagttagcac aagttgaatc tcggcaaatt acttccgaaa ttagaggtgg
      301 ggagtttgtt gacgatgatt ttaggcaact tgtagagatg cttaagctga atatgatttg
      361 cacatgggat cttcggtcta gcgtgtatta cttaacaatg aaaatgcttg gtcagcccgg
      421 tactcctgaa tatgcgaaat atagtgaact aagtgcatcg atttttaatg aattatcaga
      481 tgcttctgat acgaaagggt actggtcaac cgatgtgaag ctagtgagtt catctggaca
      541 tgaatatact gagcgtgaat ttgaactaga aagggaaaaa tctactcgcg gtaaaggagg
      601 tatgactcgt tcacttgaaa tgtttatcgc atcgctaaat tggttggcat ataaatctat
      661 ttattacagc gtcattgcta agcattttaa agcagactca tttattcatc caataaggca
      721 tgcatatcaa ttgcattgga tgaaaaaaac aggtgctttt ggtcatgatt atactgcaag
      781 gttggtgcaa tcgttgtctg ataaaatcag tactgcaaga tctgaaattg tagatcatgg
      841 acgcacatct actatttcat tagatttacc aatattttct gcatggctag caaacgaaac
      901 aggaaatgtc tctcaagtta tcaattcagc tttagagctt agaacacatg atcattttcg
      961 tacgtgtcga gatgtaatcc gagagattca tgtgacctac gatgaatcg
//
LOCUS       DAZKIP010000354         1004 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZKIP010000354 DAZKIP010000000
VERSION     DAZKIP010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.358"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>1004
                     /locus_tag="ACHOUK_005087"
     CDS             <1..>1004
                     /locus_tag="ACHOUK_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFP9900313.1"
                     /translation="SLPAAPLVRYTYTEAGELLAVYDRSNTQVRAFTYDAQHPGRMVG
                     HRYAGRPEMRYRYDDAGRVVEQLNPAGLSYHYQYEQDRITVTDSLNRREVLHTEGGAG
                     LKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTEYGLNVVSGDITDITTPDGRET
                     KFYYNDGNQLTAVVSPDGLESRRAYDEPGRLVSETSRSGETVRYRYDDAYSELPATTT
                     DATGSTRQMTWSRYGQLLAFTDCSGYQTRYEYDRFGQMTAVHREEGISLYRRYDNRGR
                     LISVKDAQGHETRYEYNAAGDLTAVITPDGNRSETQYDAWGKAVSTTQGGLTRSMEYD
                     L"
BASE COUNT          229 a          281 c          353 g          141 t
ORIGIN      
        1 agcctgcccg ctgcgccact ggtgcggtac acgtatacgg aagccggtga actgctggcg
       61 gtatatgacc gcagcaatac gcaggtgcgc gctttcacgt atgacgcgca gcacccgggc
      121 cggatggtgg ggcaccgtta tgcgggaagg ccggagatgc gctaccgcta cgacgatgcg
      181 gggcgggtgg tggagcaact gaacccggca ggcctgagtt accactacca gtatgagcag
      241 gaccgcatca ccgtcacgga cagcctgaac cggcgtgagg tgctgcatac agaaggcggg
      301 gccgggctga agcgggtggt gaaaaaagaa ctggcggacg gcagcgtcac gcacagcggg
      361 tatgacgcgg caggaaggct aacggcgcag acggacgcgg cgggacggcg gacagagtat
      421 ggtctgaatg tggtatccgg cgatatcacg gacatcacca caccggacgg gcgggagacg
      481 aaattttact ataacgacgg gaaccagctg acggcggtgg tgtccccgga cgggctggag
      541 agccgccggg catatgatga accgggcagg ctggtatcgg agacatcgcg cagcggggag
      601 acagtacgct accgctacga tgatgcgtac agtgagttac cggcgacgac aacagatgcg
      661 acgggcagca cccggcagat gacctggagc cgctacggtc agttgctggc gttcaccgac
      721 tgctcgggct accagacccg ctatgaatac gaccgcttcg gccagatgac ggcggtccac
      781 cgcgaggaag gcatcagcct ttaccgccgc tatgacaacc gtggccggtt aatctcggtg
      841 aaagacgcac agggccatga aacgcggtat gagtacaacg ccgcaggcga cctgactgcc
      901 gttatcaccc cggacggcaa ccggagcgag acacagtacg atgcgtgggg aaaggcggtc
      961 agcaccacgc agggcgggct gacgcgcagt atggaatacg acct
//
LOCUS       DAZKIP010000355         1004 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZKIP010000355 DAZKIP010000000
VERSION     DAZKIP010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.359"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>1004
                     /locus_tag="ACHOUK_005088"
     CDS             <1..>1004
                     /locus_tag="ACHOUK_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFP9900314.1"
                     /translation="SLPGAPLARYTYTEAGELLAVYDRSNTQVRAFTYDAQHPGRMVA
                     HRYAGRPEMRYRYDDTGRVVEQLNPAGLSYRYQYEQDRITVTDSLNRREVLHTEGGAG
                     LKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTEYGLNVVSGDITDITTPDGRET
                     KFYYNDGNQLTAVVSPDGLESRRAYDEPGRLVSETSRCGDVIRYAYDNPHSELPATTT
                     DATGSTRQMTWSRYGQLLAFTDCSGYQTRYEYDRFGQMTAVHREEGISRYRRYDNRGR
                     LTSVKDAQGHETRYEYNAAGDLTAVITPDGNRSETQYDAWGKAVSTTQGGLTRSMEYD
                     L"
BASE COUNT          225 a          279 c          352 g          148 t
ORIGIN      
        1 agcctgcccg gtgcgccact ggcgcggtac acgtatacgg aagccggtga actgctggcg
       61 gtatatgacc gcagcaatac gcaggtgcgc gctttcacgt atgacgcgca gcatccgggc
      121 cggatggtgg cgcaccgtta cgcgggaagg ccggagatgc gctaccgcta cgacgatacc
      181 gggcgggtgg tggagcagct gaacccggca ggcctgagtt accgctacca gtatgagcag
      241 gaccgcatca ccgtcacgga cagcctgaac cggcgtgagg tgctgcatac agaaggcggg
      301 gccgggctga agcgggtggt gaaaaaagaa ctggcggacg gcagcgtcac gcacagcggg
      361 tatgacgcgg caggaaggct cacggcgcag acggacgcgg cgggacggcg gacagagtac
      421 ggtctgaatg tggtgtccgg cgatatcacg gacatcacca caccggacgg gcgggagacg
      481 aaattttact ataacgacgg gaaccagctg acggcggtgg tgtccccgga cgggctggag
      541 agccgccggg catatgatga accgggcagg ctggtatcgg agacatcgcg ctgtggggac
      601 gtcatccggt atgcttatga taatccgcac agtgaattac cggccacgac aacagatgcg
      661 acgggcagca cccggcagat gacctggagc cgctacgggc agttgctggc gttcaccgac
      721 tgctcgggct accagacccg ttatgaatac gaccgctttg gtcagatgac ggcggtccac
      781 cgtgaggaag gtatcagccg ttaccgccgc tatgacaacc gtggccggtt aacctcggtg
      841 aaagacgcac agggccatga aacgcggtat gagtacaacg ccgcaggcga cctgactgcc
      901 gttatcactc cggacggcaa ccggagcgag acacagtacg atgcgtgggg aaaagcggtc
      961 agcaccacgc agggcgggct gacgcgcagt atggagtatg acct
//
LOCUS       DAZKIP010000356         1003 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZKIP010000356 DAZKIP010000000
VERSION     DAZKIP010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.360"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            94..465
                     /gene="ler"
                     /locus_tag="ACHOUK_005089"
     CDS             94..465
                     /gene="ler"
                     /locus_tag="ACHOUK_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE master regulator
                     Ler"
                     /protein_id="HFP9900315.1"
                     /translation="MNMENNSHTTSPYIQLIEQIAVLQQEAKRLREQEVQSVIESIQK
                     QITYYNITLQELGYTNVPDDGLARRNSSKGVYYRNEEGQTWSGVGRQPRWLKEALLNG
                     MKKEDFLVKDTEEEIIPLKNI"
     gene            480..698
                     /gene="escE"
                     /locus_tag="ACHOUK_005090"
     CDS             480..698
                     /gene="escE"
                     /locus_tag="ACHOUK_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YscE family type III secretion system
                     co-chaperone EscE"
                     /protein_id="HFP9900316.1"
                     /translation="MITITELEDEIIKNKEAANVFIEKINDKKNEIHEKMKHPLDKVT
                     YNEAKELLIACDAAIRTIEIMRIRINNK"
     gene            711..>1003
                     /gene="cesAB"
                     /locus_tag="ACHOUK_005091"
     CDS             711..>1003
                     /gene="cesAB"
                     /locus_tag="ACHOUK_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesAB"
                     /protein_id="HFP9900317.1"
                     /translation="MSQTRNKELLDKKIRSEIEAIKKIIAEFDVVKESVNELSEKAKT
                     DPQAAEKLNKLIEGYTYGEERKLYDSALSKIEKLIETLSPARSKSQSTMNQRN"
BASE COUNT          415 a          147 c          207 g          234 t
ORIGIN      
        1 ctcacataat ttatatcatt tgattaattg ttggtccttc ctgataaggt cgctaatagc
       61 ttaaaatatt aaagcatgcg gagattattt attatgaata tggaaaataa ttcacataca
      121 acaagtccat acattcagct tatagagcaa attgcagttc tacagcagga agcaaagcga
      181 ctgcgagagc aggaagttca aagtgtaatt gagtcgattc agaagcagat tacttattac
      241 aatataacct tacaagagct gggatatact aatgtgcctg atgatggact cgctcgccgg
      301 aactcatcga aaggtgttta ctaccgcaat gaagaagggc agacctggtc gggcgtaggc
      361 cgacagccac gctggcttaa agaagcactg ttgaatggaa tgaagaaaga agattttctt
      421 gtgaaggaca ctgaagaaga aataataccg ctgaaaaata tttaacatga aataattaaa
      481 tgataacgat aactgagctg gaagatgaaa taataaaaaa taaagaagcc gcaaatgttt
      541 ttattgaaaa aataaacgac aaaaagaacg aaatccatga aaaaatgaaa caccctttgg
      601 ataaagttac ctacaatgaa gcaaaggaac tgctcattgc gtgtgatgcg gcaattagga
      661 ctatagagat aatgcgaatc aggattaata ataaatagag gatgagtatt gtgagccaaa
      721 caagaaataa agaactatta gataaaaaaa taagatcaga aattgaggcg ataaaaaaaa
      781 taatcgccga atttgatgtc gtaaaagaaa gcgttaatga gttaagtgaa aaagcaaaaa
      841 cagatcctca ggcggcagaa aaattaaaca aattaattga ggggtacaca tatggagaag
      901 agcgaaaatt atatgattct gccctaagta agattgaaaa actaatagag acactgagtc
      961 cagcaagatc taaaagccaa tcaacaatga atcaacggaa tag
//
LOCUS       DAZKIP010000357         1001 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZKIP010000357 DAZKIP010000000
VERSION     DAZKIP010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.361"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..272
                     /gene="escR"
                     /locus_tag="ACHOUK_005092"
     CDS             <1..272
                     /gene="escR"
                     /locus_tag="ACHOUK_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscR"
                     /protein_id="HFP9900318.1"
                     /translation="KLGNETILKKDSLFILLPAFTMGQLEAAFKIGFLLYLPFIAIDL
                     IISNILLALGMMMVSPVTISIPFKILLFILVGGWQKLFEFLLVVN"
     gene            272..541
                     /gene="escS"
                     /locus_tag="ACHOUK_005093"
     CDS             272..541
                     /gene="escS"
                     /locus_tag="ACHOUK_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscS"
                     /protein_id="HFP9900319.1"
                     /translation="MDTGYFVQLCVQTFWIIFILSLPTVIAASVIGIIISLVQAITQL
                     QDQTLPFLLKIIAVFATLALTYHWMGTTIINFSSIIFEMIPKVNG"
     gene            541..>1001
                     /gene="escT"
                     /locus_tag="ACHOUK_005094"
     CDS             541..>1001
                     /gene="escT"
                     /locus_tag="ACHOUK_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscT"
                     /protein_id="HFP9900320.1"
                     /translation="MNEIMTVIVSSFYCILRPLGMFIILPIFSTGVLLSNFIRNSIMI
                     AFTLPIIVENYTFSEKLPSGIFQLTGIALKEISIGFFIGLSFTILFWAIDAAGQIIDT
                     LRGSTISSIFNPSISDSSSITGVILYQFISVIFVIHGGIQSILDKLYLSYE"
BASE COUNT          277 a          174 c          186 g          364 t
ORIGIN      
        1 aaaaattggg taatgaaact atcttaaaaa aagactctct atttatactg ttaccggctt
       61 tcacgatggg gcagcttgaa gctgcattca agataggttt tttgctctat ttacccttta
      121 ttgcgataga tttgatcatt tccaatatct tattggcctt gggtatgatg atggtatcgc
      181 cagtaacaat ttcgattcct tttaagatct tattattcat tcttgttgga ggctggcaaa
      241 aattattcga atttctgttg gtggtgaatt gatggatact ggatattttg ttcaattatg
      301 tgtgcaaacg ttctggataa tatttatcct ctcattgcct acagtcatag cggcctctgt
      361 tatcggtatt attattagtc tggtccaggc tataacgcag ttacaggatc aaacattgcc
      421 ttttttgcta aaaataatag cagtgtttgc tacgcttgcc ctgacttatc actggatggg
      481 aacaacaatc atcaacttct cgtcgataat atttgaaatg attccgaagg tgaacggcta
      541 atgaatgaga taatgacggt catagtatca tcattttatt gtatactcag gccgctgggg
      601 atgtttatta tcctaccaat attttcaaca ggtgttttac tcagtaactt tattcgcaac
      661 tcaattatga ttgcgtttac gcttccgatc atagtagaga actatacatt ttcggaaaaa
      721 ttgccatctg gaatctttca gttgacagga atagcgctaa aagaaataag tattggtttt
      781 ttcattgggt tatcatttac tattcttttt tgggcaatag atgcggctgg acagattatt
      841 gatactctaa gaggctcaac aatatcttca atttttaacc cgtccataag tgattcatct
      901 tctatcactg gcgttatctt gtaccaattt atctctgtga tctttgttat tcatggtggg
      961 atacaaagca tcctagataa gctatattta tcctacgaga t
//
LOCUS       DAZKIP010000358         1000 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZKIP010000358 DAZKIP010000000
VERSION     DAZKIP010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.362"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>1000
                     /locus_tag="ACHOUK_005095"
     CDS             <1..>1000
                     /locus_tag="ACHOUK_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFP9900321.1"
                     /translation="AGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDKPLYK
                     DLIGRTKAALKKNPKNVLFAVVWMQGEFDFGGTPANHAAQFGALVDKFRADLADMAGQ
                     CVGGSADGVPWICGDTTYFWKQKNEATYQTVYGSYKNKTEKNIHFVPFMTDENGVNVP
                     TNKPEEDPDIPGIGYYGSKWRDSSATWTSQDRASHFSAWARRGIISDRLATAILRHAG
                     RVALNAGASSTVSEVRPSSPSGAEATGITTLLSYLASESEGSLKVQGWSASGGRAEVV
                     SDAEGTGGKAVKLTKEAGKSSWVLEYAAGNGAALLQKGGQIRCRFKVSGALAANQYV"
BASE COUNT          240 a          237 c          332 g          191 t
ORIGIN      
        1 atgcgggcat tctgctggtt ccgtgctgtc gtggtggttc agcgttcacc accggagctg
       61 atggcacata cagtgacgcg agtggtgctt cggagaattc aacccgctgg ggggtggaca
      121 agccgctgta taaggacctt atcggtcgaa caaaagcagc actgaagaag aacccgaaaa
      181 atgtgctgtt tgccgtggtg tggatgcagg gggaatttga ttttggcggt acgccggcaa
      241 atcacgcagc acagtttggt gcgctggttg ataaattccg tgcagacctg gcggatatgg
      301 caggtcagtg cgtcggtggc tctgctgacg gtgttccctg gatatgcggg gacacgacgt
      361 atttctggaa gcagaagaac gaagccacct accagacggt gtacggcagc tacaaaaaca
      421 aaacggaaaa aaatatccat ttcgtaccgt tcatgacgga tgagaacggg gtgaatgtgc
      481 cgacgaacaa accggaagaa gacccggaca ttccgggtat cggatattac ggttcgaaat
      541 ggcgtgacag ctcagccacc tggacgtcac aggacagggc gagccatttc agcgcctggg
      601 cacgccgtgg gattatttcc gaccgtctgg caacggcgat tttgcgccat gcgggaagag
      661 tggcgctaaa cgcgggggca tcatcgacag tatcagaggt gcgcccgtca tcgccttccg
      721 gtgcagaagc cacaggcatc acaacactgc tctcttacct tgccagcgag tcagagggaa
      781 gcctgaaagt acagggatgg tcagccagtg gcggcagggc agaagtggtc agcgatgcgg
      841 agggaaccgg aggtaaggca gtgaagctga ccaaggaagc cggtaaaagc agctgggtgc
      901 tggagtacgc cgcgggcaac ggtgcggctc tgttacagaa aggggggcag attcgctgcc
      961 gctttaaggt ttcgggagcg ctggctgcga accagtatgt
//
LOCUS       DAZKIP010000359          983 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZKIP010000359 DAZKIP010000000
VERSION     DAZKIP010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.363"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(112..801)
                     /locus_tag="ACHOUK_005096"
     CDS             complement(112..801)
                     /locus_tag="ACHOUK_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFP9900322.1"
                     /translation="MNTQYLQYVREQLMAATADLNGATKGQLEAWQEHAQFDTGTYKR
                     KKPRILDVVTGKMITLDNTPTSGKQSYAKGSSIALVSPVEFSTSSWRRAVLSLDEHQK
                     AWLLWCYSESVRWGHQVTITQWAWSEFKDLLSNRKIAGKTLDRLKTLIWLAAQDVKSE
                     LAGREAYEYQTLASLVGVTTKNWSETFTERWVAMKHIFLQLDSDALLLVTRTRSKQKA
                     AFLQQNIAKLD"
     gene            complement(816..938)
                     /locus_tag="ACHOUK_005097"
     CDS             complement(816..938)
                     /locus_tag="ACHOUK_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000971054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFP9900323.1"
                     /translation="MMSESYISELLRCRWGLLCLCRFPDSVLNDYRMLKNYAKI"
BASE COUNT          261 a          251 c          190 g          281 t
ORIGIN      
        1 gcagccagca tgtttaccgt acaagtacaa ctcagggcat aaaaaaaccc actcggcagc
       61 gggtttatac attttttaca acataccaaa tttgcatgaa gcatatggct tttaatccag
      121 ttttgcaata ttttgctgta aaaatgctgc cttttgtttt gaacgtgttc tcgtcacaag
      181 caataaagca tcactatcaa gctgtagaaa aatgtgcttc attgcaaccc agcgttcagt
      241 aaatgtctcg gaccagtttt ttgttgtcac tcccaccaat gatgccagtg tctggtattc
      301 ataggcctca cgccctgcaa gttcgctctt cacatcctgt gcagccagcc agattaacgt
      361 cttcaggcga tccagtgtct tacctgcaat ttttctgtta cttaacaaat ctttaaactc
      421 gctccatgcc cattgcgtta tggtgacctg atgcccccat cgaacgcttt cgctgtaaca
      481 ccaaagcaac catgctttct gatgttcatc gagagacaaa accgcgcggc gccatgaaga
      541 ggttgagaat tcaaccgggc tgaccaaagc aatggatgaa ccttttgcgt acgactgctt
      601 accggaagtc ggcgtattat ccagcgtaat catcttgcca gttaccacat ccagaatgcg
      661 cggcttcttt cgtttgtatg taccagtatc aaattgtgca tgctcctgcc aggcttcgag
      721 ctggcctttc gttgctccgt tcaagtcagc agtagctgcc ataagttgct cacgaacata
      781 ctgtaaatat tgggtattca tgcagtaaat cctttctata ttttggcata attcttcaac
      841 attcggtaat cgttcaaaac cgaatcgggg aaacgacata agcacaggag cccccagcga
      901 cagcgaagga gttctgatat ataagactca gacatcattc attccccggt tctccaatat
      961 ctgtttcact catcatccat aac
//
LOCUS       DAZKIP010000360          975 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZKIP010000360 DAZKIP010000000
VERSION     DAZKIP010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.364"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(419..>975)
                     /locus_tag="ACHOUK_005098"
     CDS             complement(419..>975)
                     /locus_tag="ACHOUK_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4765 family protein"
                     /protein_id="HFP9900324.1"
                     /translation="DKPYSQLREGDIGYDEDLLDIYDDSPVLEDTLPALSSEIVPTPE
                     HSIKQIYSKIKEGHIELSDSDIILCRGTTGIQAENIVEYKTAGGLPDSNPNVKAPDEY
                     MAQQQVRIGRILPEYTSDLGVADRFSREHYLIVVKVKAKYITRGSVTESGWVIDKTAP
                     VEPLAIIDRTFGMKENISMVNASK"
BASE COUNT          288 a          212 c          174 g          301 t
ORIGIN      
        1 taccgtccaa aatactccgg ttccacatat aaatattctt aatgatagca aaaaatatat
       61 tttcgatata atcaatgtta tgattaaaga gtatttcatc agggcaggta aaaacagagt
      121 aaatcagcaa aagaagctga tcttcagcga tactgacact aactgacggt ttaagcggtc
      181 gtatgaagca gcagctttcc gacggactgc catgcggatc gtttaccttt tgggctattc
      241 cgcccgtcat caagcggctc acgagtactg agtttatcag gatgatattg ctgacaatgg
      301 taattcgttg accgatgtgt acttcactac atatcggtca acaccgtact gccgtgcaac
      361 atctccaaga tgtagcatcg tttttaccag aggaggcagg acatagattg taaaaaaact
      421 atttcgatgc atttaccatt gagatatttt ccttcatacc aaaagttcta tcaattatcg
      481 caagtggttc aacaggtgcg gtcttatcta taacccaacc actctctgta acacttcctc
      541 gtgtgatata ttttgccttt actttaacaa ctattagata atgctcacga ctaaaccgat
      601 cagcaacgcc aagatccgat gtgtattcag gcaatattct tccaatacgt acctgctgtt
      661 gtgccatata ttcatctggt gcttttacat ttggatttga atcaggaagc cctccagcag
      721 ttttatattc aacgatattt tcagcttgaa taccggttgt gccgcgacac aatatgatgt
      781 ctgaatcgga cagttctatg tgcccctcct taattttcga ataaatttgt ttaatactat
      841 gttctggagt tggtactatt tcagaagata aagcaggcaa cgtgtcttca agcaccggag
      901 aatcatcgta tatatccaac aaatcctcat catacccaat atctccttct cttaactgag
      961 aatacggttt atcta
//
LOCUS       DAZKIP010000361          970 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZKIP010000361 DAZKIP010000000
VERSION     DAZKIP010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..970
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.365"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            222..>970
                     /locus_tag="ACHOUK_005099"
     CDS             222..>970
                     /locus_tag="ACHOUK_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HFP9900325.1"
                     /translation="MSALLSPLSLQAADVRRSGDEAFIIQQQRQEALEQQLTPSAPDV
                     RLSAPGSFAHKINFPVETPCFQIKQTELKGADALPHWLPLQKIANGAVGHCLGAKGIN
                     LLMSTLQNRLVDHGYVTPRVLAPSQDLKSGILRLVIIPGVVRHVRLTPDSDDYIQLYS
                     SFPAHEGSLLDLRDIEQGLDLGNSRIQGQHTELNATSGNLSTQNAQLSADTLSARTAG
                     QFSSNGGTINADTLQISAQSLSNRKGSLIQTG"
BASE COUNT          228 a          229 c          257 g          256 t
ORIGIN      
        1 cgttgacgga cctcggggga aaaacgtgta tttttagtca tcctgtttac ctctttctca
       61 gggagtttag tctccaggat tcccggggcg gttcatggtg attgttggta tgacatgaat
      121 aaaaggtttt tcgtttgact ggaaaattat gttgcaggaa cgagaggact gaggaacaaa
      181 gcttattccg gcgctgtccg tccttgctgt cactgacact gatgtctgct ttattatcgc
      241 ctttatctct tcaggcagcg gatgtccggc gtagcggaga tgaagcattt atcattcagc
      301 agcagcgtca ggaagccctt gagcaacaac tgacgccttc agcccctgat gttcgccttt
      361 ctgcacctgg ctcttttgcc cataagatta attttcctgt tgaaacgccc tgttttcaga
      421 ttaaacagac ggaactgaag ggggctgatg cgttaccaca ctggctgcct ttacaaaaaa
      481 tcgccaacgg ggcggtcggg cattgcctgg gggcgaaagg aattaatctg ctgatgagta
      541 cattgcagaa ccgtctggtc gatcatggtt atgtcacccc ccgtgttctg gcaccttcgc
      601 aggatttaaa aagcggtatc ctccggctgg ttattattcc cggtgttgtg cgacatgtgc
      661 gtctgacacc ggacagtgat gactatattc agttgtattc ctcattcccg gcacacgaag
      721 gttctctgct ggatttacgg gacattgagc aggggctgga tttaggtaac agccggatac
      781 agggacaaca tactgagctg aatgcaacca gtggaaatct gtctacacag aatgcgcaac
      841 tgagtgccga tacgctttcc gcccggactg ccgggcagtt cagcagtaat ggcggtacga
      901 taaatgccga cacactgcag atatcggcac aaagcctgtc aaatcgtaaa ggcagtctga
      961 ttcagacggg
//
LOCUS       DAZKIP010000362          946 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZKIP010000362 DAZKIP010000000
VERSION     DAZKIP010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..946
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.366"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            21..830
                     /gene="yqeI"
                     /locus_tag="ACHOUK_005100"
     CDS             21..830
                     /gene="yqeI"
                     /locus_tag="ACHOUK_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9900326.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSYLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQHEVAGCLTLSYREVNRG"
     gene            827..>946
                     /locus_tag="ACHOUK_005101"
     CDS             827..>946
                     /locus_tag="ACHOUK_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900327.1"
                     /translation="MIIKKNLLFILVFISGFILFTVYSYTAEKMIYNETCTANW"
BASE COUNT          310 a          178 c          180 g          278 t
ORIGIN      
        1 tatttagtgt ttattgacgt atgtactgga ttattaacga taatatcgag ttctggcctg
       61 agcaccgaaa attaatatcg gtacataacg ccgatcttaa cgtcgttctg acaacgccag
      121 ccagtcgttg tttatcactg ctacttgaag cttttcctga tgtggttgca caacaagatt
      181 ttttcaccag agtctgggaa gaagaaggta tgcgtgtgcc tactaacacg ttatatcaga
      241 acatatccat tatcagacgc ggatttcgcg ctgttggtga tactacccac tcgcttattg
      301 caaccgtgcc gagaagagga ttcaagatcc ataatgacat taacatacaa aatcatgtaa
      361 taaactcatc aacagacgca catacacaca atgccccacc tgccataaaa gttaatgcgg
      421 gatacaaaga gagcattggt ggcgcaaaga atttcaataa caaaatcctc aaacatataa
      481 aaagttatct aattatgttg agcgcatttg tcataggcgc atattctgca tattggctat
      541 ggaataataa tcaaccgaag ccatttttca aggattacaa gactgttgcg gaaattaatg
      601 gctgccattt taatgtaaca gaagatacaa ttgatgggtt gaaagagttc gataaatata
      661 aaacacggat actggattcg gggattaact gcaaaaaata tccgtggtta tacttccctc
      721 ttgcaaaatc ctcaccgggg atgattgtta tggcatgcaa taaaaactat aaccaacatg
      781 aagtggcagg ctgtttaacc ctgtcttacc gtgaggttaa tcgtggttga ttataaaaaa
      841 aaacttatta tttattttag tttttatcag tgggtttatt ctcttcactg tctattctta
      901 cactgcagaa aaaatgatat ataacgaaac atgcactgca aattgg
//
LOCUS       DAZKIP010000363          916 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZKIP010000363 DAZKIP010000000
VERSION     DAZKIP010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.367"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..865)
                     /locus_tag="ACHOUK_005102"
     CDS             complement(<1..865)
                     /locus_tag="ACHOUK_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFP9900328.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEGW
                     EDIEDFGETHLDFLRQYGDFENGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSDD
                     KDVIAIDGKTLRHSYDKSRRRGAIHVISAFSTMHSLVIGQIKTDEKSNEITAPPELLN
                     ILDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNP
                     EHDSYAISEKSHGREEIRLHIVCEVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKE
                     PKMTVRYYISSA"
BASE COUNT          247 a          204 c          161 g          304 t
ORIGIN      
        1 cagcagaact gatataatat ctgaccgtca tttttggctc tttcttttgt tctgctatta
       61 tggaccgaaa ggagactgcc acgcataatt tcttcagccc tttccattca aacgtgaaat
      121 caataagttc atcagggact tcgcaaacaa tatgaagacg gatttcttct ctgccgtgac
      181 tcttttcact aattgcgtaa ctgtcatgct ctggattatt taattctttc agcggaaatt
      241 tttcctcaaa ggctttattt agccgcccct ggtttccttt taccgcgaat aaataatcac
      301 ctccctgttt ttgtatcttc tctgcaatat ctttctggca ccccatcgca tcagttgtga
      361 tgatttttcc tttaatatcc agtatgttaa gaagttcagg gggagctgta atctcattag
      421 atttctcatc cgtcttgatc tgtccgatga ccagactgtg cattgttgag aacgcactaa
      481 tgacatgaat cgctcccctg cggcgactct tgtcataaga gtgccggagc gtttttccat
      541 caattgcaat gacgtcttta tcatctgaag aatggcagtc acgcatccag ttaataaagc
      601 actcatgaaa ttttgcagga ctgatacagg atacaactct ggcaatggta tcgtggacag
      661 gaataccatt ttcaaaatca ccatattgcc tcaaaaaatc gagatgcgtt tcaccaaaat
      721 cctctatatc ttcccaacct tctgcaccag aaatgacggc acaaatagtc aacagtagaa
      781 tatctgacaa tttatgttct actttccagg cttgtctgta atcggggata atagaaatat
      841 gttccatcaa ttttttaagt tccattttgt tctccttatt tatgcaagga gtatttgatc
      901 atatgtaagc tgtgaa
//
LOCUS       DAZKIP010000364          909 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZKIP010000364 DAZKIP010000000
VERSION     DAZKIP010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..909
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.368"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..>909)
                     /locus_tag="ACHOUK_005103"
     CDS             complement(<1..>909)
                     /locus_tag="ACHOUK_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900329.1"
                     /translation="KTSITDREKNIYPVICDSPDHFTSDIYRHPFRIVLGNKTLYPSE
                     ELVKFISTSKEYLSNMDVINNVIVPQKTTKKNKLSIVSLNSNIKNDIVLSGVMTGTSK
                     IFHLNNSGDLLLTTSKTHGGGVVVIFKDFINNWWKYNLTLITVPIDNKLSDNRINITP
                     MGIKIQETVSGNDRLFFYPTPLKNGCFILHNPLYSNSFPSLYSHELFDLIEAYRNPTY
                     SYLQNNIINRYIHTVSEYAGKDGIANMSLSIYAYSTCRFWSKESVAVGDTIKLSNYEH
                     IEFSFNSFSKDYFREQNSDIYKIFFK"
BASE COUNT          302 a          140 c          128 g          339 t
ORIGIN      
        1 attttaaaaa atattttata tatatcacta ttttgttcac gaaagtaatc cttactaaat
       61 gaattaaagc taaactcaat atgctcataa ttagacaatt ttattgtatc tcctacagcc
      121 acactttctt ttgaccagaa acgacatgtc gagtatgcgt atatagataa tgacatattg
      181 gcaataccgt cttttccagc atactcagaa acagtatgta tatatctatt gataatattg
      241 ttctgtaaat acgaatacgt tggatttcta tatgcttcaa tcaaatcaaa taattcatgt
      301 gaatataaag aagggaatga gttagagtaa agtggattat gcaagataaa acatccattt
      361 tttaatggag tagggtaaaa aaacaacctg tcatttccac tgactgtttc ctgtatcttt
      421 atccccattg gggttatatt tattctattg tctgacaatt tattatcaat aggaactgtt
      481 attaaagtaa gattatattt ccaccagtta tttataaaat ctttaaaaat aaccacaacc
      541 ccaccaccat gagtttttga ggttgttaac aataaatcac ctgaattatt tagatgaaaa
      601 atctttgatg ttccggtcat tactccggac aatacaatat catttttgat attgctattt
      661 aaactaacta tggataattt gtttttcttt gttgtttttt gaggaacaat aacattgtta
      721 atcacatcca tattgcttaa atattctttt gaggtgctta tgaattttac aagttcttct
      781 gatggataca atgtcttatt cccgagaact attctaaatg gatgcctata tatgtcgctt
      841 gtgaagtgat caggtgaatc acaaatcaca ggatatatat tcttttctct gtcagtgata
      901 cttgtttta
//
LOCUS       DAZKIP010000365          909 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZKIP010000365 DAZKIP010000000
VERSION     DAZKIP010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..909
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.369"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>909
                     /locus_tag="ACHOUK_005104"
     CDS             <1..>909
                     /locus_tag="ACHOUK_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFP9900330.1"
                     /translation="AGQSNAMAYGEGLPLPDSYDAPDPRIKQLARRSTVTPGGAACRY
                     NDIIPADHCLHDVQDMSTLNHPKADLSKGQYGCVGQGLHIAKKLLPYIPNNAGILLVP
                     CCRGGSAFTQGAEGTFSADAGASQDSARWGVGKPLYQDLIARTKAALQKNPKNVLLAV
                     CWMQGEFDMSAATHAQQPALFTAMLAQFRADLSVFNAQCHGGSAADVPWICGDTTYYW
                     KNTYGTQYNTIYGAYKNRESEGVYFVPFMTDGNGVNTATNAPAEDPDIPASGYYGAAS
                     RTNGNQVSSNRPTHFSSWARRSIIPDR"
BASE COUNT          211 a          236 c          273 g          189 t
ORIGIN      
        1 gccgggcagt ccaatgccat ggcttacggt gaagggcttc cgctgccgga ttcatacgat
       61 gctccggatc cgcgcattaa acagctggcg cgccgcagta cagttacgcc gggtggggct
      121 gcctgcagat ataacgatat tattccggcc gaccactgcc tgcatgatgt gcaggatatg
      181 agtacgctga atcatccgaa ggcagacctg agcaaagggc agtacggctg tgtcggccag
      241 ggcttacata ttgccaaaaa actgcttccg tatatcccga ataacgcggg gatcctgctg
      301 gtaccatgct gtcgtggtgg ttcggcattc acccagggcg cggaggggac attcagtgcg
      361 gacgcggggg ccagccagga ttcggcgcgc tggggtgtgg gtaaaccgtt atatcaggac
      421 ctgattgcgc gcactaaagc tgcattacag aagaacccga aaaatgtgtt gctggcggtg
      481 tgctggatgc agggagagtt tgacatgagc gccgccaccc acgcacagca acctgcgctg
      541 tttacagcca tgctggcaca gtttcgtgct gacctctccg tgtttaacgc gcagtgccat
      601 ggtggcagtg ctgcagatgt gccgtggatt tgtggtgaca cgacgtatta ctggaaaaat
      661 acctacggca cccagtacaa caccatttac ggggcgtaca aaaacaggga gagtgagggc
      721 gtttattttg tgcccttcat gacagacggt aacggcgtca ataccgccac taacgcgccg
      781 gcagaagatc cggatattcc ggcatcagga tattacggtg cggcatcgag aacgaatgga
      841 aaccaggtat catcaaaccg cccgacacat ttcagttcat gggcgcgcag gagcattatt
      901 ccggatcgt
//
LOCUS       DAZKIP010000366          900 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZKIP010000366 DAZKIP010000000
VERSION     DAZKIP010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.370"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..327
                     /locus_tag="ACHOUK_005105"
     CDS             <1..327
                     /locus_tag="ACHOUK_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP9900331.1"
                     /translation="GASMALWGSALAAGGFSATTMLFSLGASMILGGVAQMLAPKAKT
                     PDYRATDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGDGKVV
                     VIGRQA"
     gene            587..>900
                     /locus_tag="ACHOUK_005106"
     CDS             587..>900
                     /locus_tag="ACHOUK_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900332.1"
                     /translation="MGKGGGRAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTR
                     TIT"
BASE COUNT          232 a          211 c          290 g          167 t
ORIGIN      
        1 ggggcatcaa tggcgttatg gggttcagcc ctggcagccg gtggtttttc tgccaccacg
       61 atgctgtttt cactgggggc cagcatgata ctgggcggtg tggcccagat gctggcaccg
      121 aaggcaaaaa caccggatta ccgcgcaacg gataacggca gacagaacac gtacttttcc
      181 tcactggaca acatgattgc tcaggggaac ccgatgccgg tgccttacgg tgaaatgctg
      241 gttggctcac ggcgaatctc ccaggacatc agtacccgtg atgaaggcgg tgacgggaag
      301 gtggtggtta tcgggcggca ggcataaaag cgaaaaaatc ccgcagtgac cgaaggctgc
      361 gggaacagaa aatgaagatt aaccacaggg agttttgttt ttattggccc gaaaaaactg
      421 taacgcccgg gaatgatatc tgccacgggg gcgtacagaa aatgtgaaga aattcagaaa
      481 ttttattccg tcatgacaca ggcaccctcc ggggtgcctg tcgtttttgg ggcataaaca
      541 gattcagaca tcagacatca gacatcagac aggagagggg gaccgagtgg gtaaaggtgg
      601 cggcagggca cacacgccgg ttgaggcaaa ggacaatctt aagtccacgc agatgatgag
      661 cgtgattgat gccattggtg aagggccgat tgaaggtccg gtgaaggggc tgcagagtat
      721 cctggtgaac aaaaccccgc tgacggacac ggacggtaat cctgtgatac atggtgtgac
      781 cgcggtctgg cgcgccgggg agcaggagca gacaccacct gaaggctttg agtcctccgg
      841 ggcggaaacc gcactgggcg tggaagtgac gaaggcaaag ccggtgacgc gcaccattac
//
LOCUS       DAZKIP010000367          898 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZKIP010000367 DAZKIP010000000
VERSION     DAZKIP010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..898
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.371"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            45..845
                     /locus_tag="ACHOUK_005107"
     CDS             45..845
                     /locus_tag="ACHOUK_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900333.1"
                     /translation="MNSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVARAENMNWMQALRFVAAKGHQKAIIV
                     YQDMLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     SRVIFPPAGITKTSNKELSNDHVTEAQLFNLKQKTYVSMVKDLVSRFQ"
BASE COUNT          285 a          176 c          206 g          231 t
ORIGIN      
        1 gctaaatatt ctaatcatta tgacaggcga gggagtgtcc aattatgaat tcaaaaaagc
       61 tttgttgcat atgtgtgtta ttctcgctgc ttgcaggatg tgcctctgaa tcttctattg
      121 atgaaaagaa gaaaaaagct caagtcacac aaagtaatat taataaaaac actccccagc
      181 aactgacaga caaagattta ttcggtaatg aaaccactct ggccgtatcc gaagaggata
      241 ttcaagcagc gcttgatgga gatgagttcc gcgttcccct taattctcct gtaatacttg
      301 ttcaatccgg caaccgcgca ccggaaacca ttatgcagga ggagatgcga aaatattata
      361 ctgtttccac attctctggt atcccggaca ggcaaaagcc tctgacttgt aacaaaaaca
      421 aagataaaaa tgagaacgag gatgttgccc gtgctgagaa tatgaactgg atgcaggcac
      481 tgcgttttgt ggctgcaaaa ggacatcaga aagcgattat tgtttaccag gatatgttgc
      541 agacaggaaa atatgactct gcgctgaaat caacagtatg gtccgactat aaaaatgaaa
      601 aactcactga cgctatctcc ctgcgctacc tggtacgttt cacgctggtg gatgtggcaa
      661 caggtgagtg ggctacctgg tcgccggtga attatgaatc gagagtaatt tttccgccgg
      721 ctggaataac aaaaacgagc aataaagaac tatcaaatga ccatgttacg gaagcgcaac
      781 tgtttaattt gaaacaaaaa acatatgtat cgatggtgaa agacttggtg agtcgattcc
      841 agtaatcgtt attgttatgc tgccgcctgt agtttagggt ggcagcaaac aggagttg
//
LOCUS       DAZKIP010000368          885 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZKIP010000368 DAZKIP010000000
VERSION     DAZKIP010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.372"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..463
                     /locus_tag="ACHOUK_005108"
     CDS             <1..463
                     /locus_tag="ACHOUK_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP9900334.1"
                     /translation="LLRTHQLRHLLNTFAQINGMDEFSIARWSGRKLISQNVSYDHRS
                     HLQMSKAIREKKLSVCVNEHRIKDIPVVDLNEFDSLSSGAVLVSKHGYCKHSYAFKPC
                     DNYPIKNSGLDNETISNIHDKILKRTLYDKNDGNINADKWYEFHKKIKKGE"
     gene            464..862
                     /locus_tag="ACHOUK_005109"
     CDS             464..862
                     /locus_tag="ACHOUK_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900335.1"
                     /translation="MAKHINGYHEKKIVDVINTWSIDEKLTWNALCDRLVRVIGKRPS
                     RQSLSSHVRIAESFNVKKTTIKSGVIHTVKPANLKVASQRIKRLEAENEALKALNERY
                     LEQFKVWLYNAHLKQITIEELNNPLPAKYL"
BASE COUNT          339 a          130 c          162 g          254 t
ORIGIN      
        1 tttgctaaga acacatcaac taaggcatct tctcaacaca tttgctcaaa taaatggtat
       61 ggatgaattt agtattgctc gctggtctgg acgtaagctt atttctcaaa atgtttctta
      121 tgaccacaga tcgcatcttc aaatgtctaa agcaataaga gaaaaaaagt tatcagtatg
      181 tgttaatgag cacagaataa aggatattcc agtagtggat cttaatgagt ttgactcact
      241 tagtagtggt gcagtacttg tatcaaaaca tggctactgc aagcactcat atgcgtttaa
      301 gccgtgtgat aattatccaa ttaagaactc tggtttagat aacgaaacga tttcaaatat
      361 ccacgataaa attttaaaaa gaacactgta tgataaaaat gatgggaaca taaatgctga
      421 taaatggtat gaattccata aaaaaataaa aaaaggagaa taaatggcta agcatataaa
      481 tggttatcat gagaagaaga ttgtagatgt tattaacaca tggtctatag atgaaaaact
      541 aacctggaat gctttgtgcg ataggttagt cagggttatt gggaaaagac catcaagaca
      601 atccttaagt agtcatgtaa ggattgcgga aagcttcaat gtgaaaaaaa ccacaatcaa
      661 atctggtgta atacacactg taaaacctgc taatttaaaa gtggcttctc aacgaattaa
      721 aagacttgaa gcagaaaatg aagctttgaa ggcattaaat gaacgctatt tagagcagtt
      781 caaagtatgg ctttataatg ctcaccttaa acaaataacg attgaagaac ttaataatcc
      841 gttacctgca aaataccttt aataaaattg ataggtttta gctat
//
LOCUS       DAZKIP010000369          851 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZKIP010000369 DAZKIP010000000
VERSION     DAZKIP010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.373"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            96..>851
                     /locus_tag="ACHOUK_005110"
     CDS             96..>851
                     /locus_tag="ACHOUK_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900336.1"
                     /translation="MSIDFTPGMINTYHGDIYNRTTDTDNVKTPDTPMWPCDNREEQQ
                     PINSTFSGEGYNPEQYDLARQHLPQIYACRTNTKYTDADYSKVVAQLVSLITNIESIS
                     STPLTRQAQEILNQINNIRYEKNKSAECRIIVVANPKPDKAIITKISVEEGIPVRFSV
                     QTMFSDTNFIAEQRADLPTNIKDIQSLYQKMTKLYIEHSENKNRMKVFAGTNFIDFNM
                     TGQNLSGFVLTLSRFYFEDLLNINFTDANLGDTI"
BASE COUNT          315 a          167 c          142 g          227 t
ORIGIN      
        1 catgatattt attgtgcgta gaagaaaatc cattcctgct ttaattatcc aatttaaaaa
       61 tcttagcata aaacagataa tgaataagga atgttatgtc aattgacttt accccaggta
      121 tgataaatac atatcacggc gatatttata accgcacaac agataccgac aatgttaaaa
      181 caccagacac accgatgtgg ccttgtgata atcgggaaga acaacaaccc attaattcca
      241 cattctctgg agaaggatac aaccctgaac aatatgattt agcgcggcaa cacctcccac
      301 aaatttatgc gtgccgcacg aacaccaaat atacagatgc agactactca aaagttgtgg
      361 ctcaacttgt aagtcttatt actaacattg aatcgataag ttcgacacca cttacgcggc
      421 aggcacagga aatacttaat caaattaata acattagata cgaaaagaac aaaagtgctg
      481 aatgtcgaat cattgttgtc gctaatccta aacccgacaa ggcaatcata actaaaatta
      541 gcgtagaaga gggaatacca gtaagattca gtgtccaaac catgttttca gatactaatt
      601 ttattgctga acaacgagct gacttaccga ctaatataaa agatattcaa agcctgtatc
      661 aaaaaatgac gaagttatat attgagcaca gtgaaaataa aaacaggatg aaagtctttg
      721 ccggaaccaa ctttattgat ttcaatatga caggacagaa cctgtccggg ttcgttctaa
      781 ccttgtcaag gttttatttc gaagatctac ttaatataaa tttcactgac gcaaatttag
      841 gtgacactat t
//
LOCUS       DAZKIP010000370          850 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZKIP010000370 DAZKIP010000000
VERSION     DAZKIP010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..850
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.374"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..143)
                     /locus_tag="ACHOUK_005111"
     CDS             complement(<1..143)
                     /locus_tag="ACHOUK_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFP9900337.1"
                     /translation="MFPATKGALMRIYRRKCKCCNEWFIPKYQNQYWCNEICGTKIAL
                     ERR"
     gene            complement(91..657)
                     /locus_tag="ACHOUK_005112"
     CDS             complement(91..657)
                     /locus_tag="ACHOUK_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFP9900338.1"
                     /translation="MAKPARRRCNRKREDLTVKRIFELLSFDKSTGVFRWKVPTQGRI
                     ALNSVAGAFDSNGYSMIMIDGRRYKTHVLVFYITHNRWPAGQIDHVNGIRTDNRPENL
                     RECLPIENSRNIRIRKNSKSGCRGVTWHKRQKKWNVRLGFHGKSKHFGCFDDLELAVL
                     VAEEARDKYYGDFSGNERSTYANLSKEM"
BASE COUNT          243 a          200 c          135 g          272 t
ORIGIN      
        1 cttcgtcgtt cgagtgctat cttggttcca caaatctcat tacaccaata ttgattttga
       61 tattttggta taaaccattc attgcaacat ttacatttcc ttcgatagat tcgcataagt
      121 gctcctttcg ttgccggaaa aatcaccgta atacttatct cgggcttctt cagcaactag
      181 taccgctaac tccagatcat caaagcatcc gaagtgttta ctcttgccat ggaatcctag
      241 cctaacattc cattttttct gtcgtttgtg ccaagtaacc cctctgcaac ctgatttgct
      301 attctttcgg atccttatat ttcttgaatt ttctattggc aggcattctc ttaaattttc
      361 tggcctattg tcggtcctaa ttccattaac gtggtcaatt tgaccagcag gccaacgatt
      421 atgagttatg taaaaaacta agacgtgagt tttatatcta cgcccatcta tcatgatcat
      481 tgaataaccg ttggaatcaa aagctccagc aacactattt aatgctatcc ttccctgagt
      541 gggaactttc catctaaata ccccggtaga tttatcgaaa cttagtaact caaatatcct
      601 tttaacagtt aaatcttctc ttttacggtt acatcgtctt cgcgctggtt tagccatcgc
      661 cttcttcctc cgtaatggtt ttctgaattt ggccacctga acagagctca ccaaagctat
      721 ggatgtcggt atttccacaa taccaagatg gcgaaaataa ctgcatgata agcctcaggg
      781 aaaagggaag acactacccc cgataattca gaaacaaatc gaaatacatg aactaaagaa
      841 aaaatcacaa
//
LOCUS       DAZKIP010000371          845 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZKIP010000371 DAZKIP010000000
VERSION     DAZKIP010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.375"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            56..151
                     /locus_tag="ACHOUK_005113"
     CDS             56..151
                     /locus_tag="ACHOUK_005113"
                     /inference="COORDINATES: protein motif:HMM:NF026967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTF2 fold immunity protein"
                     /protein_id="HFP9900339.1"
                     /translation="MEGKQPKNSGGNFTMLIAKKDGQVLNVIHTK"
     gene            786..>845
                     /locus_tag="ACHOUK_005114"
     CDS             786..>845
                     /locus_tag="ACHOUK_005114"
                     /inference="COORDINATES: protein motif:HMM:NF040040.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900340.1"
                     /translation="MSRIAYGTTEWNFYDNEDLE"
BASE COUNT          292 a          142 c          177 g          234 t
ORIGIN      
        1 aaaagcagaa tttcagaaac catatagtat tacagatgac aataattgtt ggaaaatcga
       61 gggaaaacaa ccgaagaatt caggaggaaa ttttacaatg ctaatagcga aaaaagatgg
      121 gcaagtgttg aatgtaatac acacaaaata atccactgta cagtcgattc aaatatttta
      181 ccaaggtgtg tttcgtaaca attttgtgta caatatgact ctacatatcg acgaaaaaac
      241 cacttgattt gacaggaata cattcaaaaa atttcttttt tcatttcagg agcgattttc
      301 atctgttact tgaagtatct gtgagattaa gtgactcccc ctaacgtcgt actaaatagt
      361 aattactggc cataccggga gcagtattcg gcgctcccca catgcagcag aataaccgca
      421 tcagcgaaga tgtattttta ctatcacaat gtgtacggac ggtttatgga aaagaatgaa
      481 cattagatac gcgatgcggc ggcaggttac acggttatca ctacgatagc caatactggc
      541 tggtgcatta ccgccgtgaa caataggacg ccgtactgct ggaaatttct tacctttata
      601 actccccgcg ccgccgtatc gggaaacggg aagcaatcaa gatgaggctg atataatgtg
      661 cggaatgcct gagtattttt agaatagggg ggtaatatgt ttgatagaat atatcttggt
      721 gatcgagcaa taaaaaaatt gaatttgatt tatggagtaa acaaattaga attcaggtta
      781 atttaatctc aaggatagcc tatggtacaa cagaatggaa tttttatgat aatgaagact
      841 tagaa
//
LOCUS       DAZKIP010000372          831 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZKIP010000372 DAZKIP010000000
VERSION     DAZKIP010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.376"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>831
                     /locus_tag="ACHOUK_005115"
     CDS             <1..>831
                     /locus_tag="ACHOUK_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HFP9900341.1"
                     /translation="YHEKAVMNYMEYLVTCQLDEEQMSSLLYERDDLSAIDFSLLMKT
                     IKAFSFGGDLQTLASKPSSTISSIPSKRRILININHDFPNNGNLFNDFLFNHQQDEQL
                     AMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWLNKGCSTYQQLYNEFH
                     ALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVEGAWPNK
                     LQPGKYKNMGSNSVNIIINNTRKIIPPGKAFMLRGGTLNINIPGRSALLLGKTGEPLN
                     "
BASE COUNT          274 a          158 c          167 g          232 t
ORIGIN      
        1 aatatcatga aaaagccgtt atgaattaca tggaatatct ggtaacttgc cagttggatg
       61 aagaacaaat gtcatcgctg ctttatgaaa gagatgattt aagcgccatt gatttttcat
      121 tgctcatgaa gacgataaaa gctttttcat ttggtggcga tctccaaacc cttgcatcaa
      181 aaccgagttc cacaatctca agcatcccat caaaaagacg gatattgatt aacattaacc
      241 acgattttcc taacaatggc aatcttttca atgactttct atttaaccat caacaagatg
      301 aacaattagc aatggcatat atggccgctc tcccgttcag caggccttta gtttactggg
      361 atggccaagt attaaaatca acgactgaaa ttaaaaatta tgatgggtcg acgcgtgtcg
      421 gcggtgaggc gtggcttaat aaaggttgct ctacctatca gcagctctac aatgaattcc
      481 acgcattata tatagataaa gcaggaatat ggagcgcatt tgagggtgta tttgcaacta
      541 aaaacgttct ggcctttagt cgtggggatt cggtgaacat taatcactct cctcatgatg
      601 gactagttat aataaataaa ggaaacgaag aagttgaagg tgcctggcct aacaaattgc
      661 aacctggaaa atataaaaac atggggagta atagcgttaa cattattatt aataataccc
      721 gaaaaattat cccccctggt aaagcattta tgcttagagg cggaactcta aatatcaata
      781 ttcctgggcg tagcgctctt cttttaggga aaacaggaga accgctgaac t
//
LOCUS       DAZKIP010000373          825 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZKIP010000373 DAZKIP010000000
VERSION     DAZKIP010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.377"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>825
                     /locus_tag="ACHOUK_005116"
     CDS             <1..>825
                     /locus_tag="ACHOUK_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1203 family transposase"
                     /protein_id="HFP9900342.1"
                     /translation="MPLLDKLREQYGVGPVCSELHIAPSTYYHCQQQRHHPDKRSARA
                     QHDDWLKREIQRVYDENHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGK
                     KVRTTISRKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYIV
                     GWRVSSSMETTFVLDALEQALWARRPSGTIHHSDKGSQYVSLAYTERLKEAGLLASTG
                     STGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLGRLGHT"
     misc_feature    <1..72
                     /locus_tag="ACHOUK_005116"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
BASE COUNT          200 a          193 c          256 g          176 t
ORIGIN      
        1 atgccactgc tggataagct gcgtgagcag tacggggtcg gaccggtatg cagcgaactg
       61 catattgccc cgtcaacgta ttaccattgt cagcaacagc gacatcatcc ggataaacgc
      121 agtgcccgtg cgcagcacga cgactggctg aagagagaga tacagcgcgt atacgatgaa
      181 aatcatcagg tgtacggtgt gcgtaaagtc tggcgtcagt tgttacggga aggaatcagg
      241 gtggccagat gtacagtggc acgtctcatg gcggttatgg gacttgccgg tgttctccgg
      301 ggtaaaaagg tccgtacgac catcagccgg aaagccgttg ccgcaggcga ccgcgtaaac
      361 cgtcagttcg tggcagaacg acctgaccag ctgtgggtgg ctgattttac ttacgtcagc
      421 acatggcagg gcttcgtcta tgtggcgttt atcattgatg tgtttgccgg atacatcgtg
      481 gggtggcggg tctcatcgtc tatggaaacg acattcgtgc tggatgcgct ggagcaggcg
      541 ttgtgggccc gtcgtccgtc tggcaccatc catcacagcg ataaaggctc tcagtatgtg
      601 tcactggcct atacggagcg actaaaagaa gccggattac tggcatcaac agggagtaca
      661 ggcgactcgt atgacaacgc gatggctgag agcatcaatg gtctttacaa agcggaggta
      721 atacaccgta agagctggaa aaaccgtgca gaagtggaac tggccacact aacgtgggtg
      781 gactggtata acaatcgacg attgctggga aggctgggcc atact
//
LOCUS       DAZKIP010000374          823 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZKIP010000374 DAZKIP010000000
VERSION     DAZKIP010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.378"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..548)
                     /gene="fimE"
                     /locus_tag="ACHOUK_005117"
     CDS             complement(<1..548)
                     /gene="fimE"
                     /locus_tag="ACHOUK_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313299.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFP9900343.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTLERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGL"
BASE COUNT          270 a          186 c          163 g          204 t
ORIGIN      
        1 cataatccgg caaaacgagc agcattactg gcggtataac gtacagtatg gcgaatattt
       61 cgatgcccga gataatcctg aattaaacga gtatctgcac cacgctccgc cagttcataa
      121 ccgcaagcat gccttaacat atgaggatga gtctgcgtta cggttccagc ttcaataccg
      181 gcatcacgaa taatgcgata ggcctgctgg cgagaaagcc gactcccgcg gcgagaaata
      241 aatatggcgt cggtccggtc agcgcctttc cagttagcac gttctagggt ccagcgttcc
      301 acagcttcac gctcatcaaa acgtaacggg tgaacggtag aaaatccgtt cttcagtcgg
      361 cgaatattta ttcggccttc attaaggtca agatcctgat aatgcagatc aagcagttca
      421 ctaatacgca tcccatgccg atatgccaac agaataagac aataatctct ggctcccgtt
      481 gccccgtaac aaaccgcctg catcatggcc tgaacttctt taccggtaag ataacgacgt
      541 ttactcacaa tagtagtact cctaactgag atatatttga atgaatacct ataggaaacc
      601 tcaatcggtc aaaattagcc caatagataa aaagataacc acacacgaag tgatgtggct
      661 atcagtcaat tacattaaat atcatacaaa aataaaatat cactgatgtg ataacatcta
      721 ttttcattga tttgattcaa tataacaata gattgcagtg gattaacttc cagttgctta
      781 aaaaataaag gaaaaagtgc agaaacagga gagtttaagt gat
//
LOCUS       DAZKIP010000375          823 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZKIP010000375 DAZKIP010000000
VERSION     DAZKIP010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.379"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..384
                     /locus_tag="ACHOUK_005118"
     CDS             <1..384
                     /locus_tag="ACHOUK_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFP9900344.1"
                     /translation="SSLPQGVALTGDGGNNLLAAFYIQTDAKDLNVMYHNAKVATNNL
                     KLGTFGAFDNEWHTLAFRFAGNNSLQVTPVIDGQDGTPFTLTQSPVSAFAADKLHVTD
                     ITRNATYPVLIDSIAVEVNNADAAA"
     gene            521..700
                     /locus_tag="ACHOUK_005119"
     CDS             521..700
                     /locus_tag="ACHOUK_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFP9900345.1"
                     /translation="MTFLNQLMLYFCTVVCVLYLLSGGYRAMRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPLI"
     gene            741..>823
                     /locus_tag="ACHOUK_005120"
     CDS             741..>823
                     /locus_tag="ACHOUK_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFP9900346.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALK"
BASE COUNT          224 a          197 c          217 g          185 t
ORIGIN      
        1 tcttcactgc cacagggcgt tgccctgacc ggagacgggg ggaataacct gctggcagcg
       61 ttctacatcc agacagatgc aaaagacctg aatgtgatgt accacaatgc gaaagtggcg
      121 acaaacaacc tgaaactggg aacctttggc gcatttgata acgaatggca tacgctggct
      181 ttccgctttg ccgggaataa cagccttcag gtgacgccgg ttattgatgg tcaggatggc
      241 acaccgttca cgctgacgca gtcaccggtc agtgcatttg cggcggataa actgcatgtg
      301 acagacatta ccaggaatgc gacttacccg gtgctgattg acagcattgc ggtggaagtg
      361 aacaacgcgg atgccgcggc atgataaaaa aagccgccag ttgcctgaga cctctggcgg
      421 tgtgagattc atggagaatc aaggaaagat actaccactt tcgtcatcgg catttttaaa
      481 tgaaaactgt ttacttagtc aaccataacg gtaagaaatt atgacatttc tgaaccagtt
      541 aatgctgtac ttctgtacgg tggtctgtgt gctgtatctc ctttcgggtg ggtacagggc
      601 catgcgtgac ttctggcgca gacagattga caaaagggcc gctgagaaaa tcagcgccag
      661 tcagtcagcc ggaagcaaac ccgaagagcc gctcatttag cggcaacttt cttaatcaca
      721 tctttcgacg agaaaatccc atgtcagaaa ttacatccct ggtcactgct gaagcagtga
      781 aggaagtcct gcgctctgaa gaagtccgga gcgcactgaa aca
//
LOCUS       DAZKIP010000376          823 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZKIP010000376 DAZKIP010000000
VERSION     DAZKIP010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.380"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..384
                     /locus_tag="ACHOUK_005121"
     CDS             <1..384
                     /locus_tag="ACHOUK_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFP9900347.1"
                     /translation="SSLPQGVALTGDGGNNLLAAFYIQTDAKDLNVMYHNAKVATNNL
                     KLGSFGAFDNEWHTLAFRFAGNNSLQVTPVIDGQDGTPFTLTQSPVSAFAADKLHVTD
                     ITRGATYPVLIDSIAVEVNSTDTAA"
     gene            521..700
                     /locus_tag="ACHOUK_005122"
     CDS             521..700
                     /locus_tag="ACHOUK_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFP9900348.1"
                     /translation="MTFLNQLMLYFCTVVCVLYLLSGGYRAVRDCWRRQIDKRAAEKI
                     SASQSAGSKPEEPLI"
     gene            627..>823
                     /locus_tag="ACHOUK_005123"
     CDS             627..>823
                     /locus_tag="ACHOUK_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFP9900349.1"
                     /translation="MTKGPQRKSAPVSQPEANPKSRSFSGNFLNHIFRRENPMSEITS
                     LVTAEAVKEVLRSEEVRSALK"
BASE COUNT          232 a          191 c          221 g          179 t
ORIGIN      
        1 tcttcactgc cacagggcgt tgccctgacc ggagacgggg ggaataacct gctggcagcg
       61 ttctacatcc agacagatgc aaaagacctg aatgtgatgt accacaatgc gaaagtggcg
      121 acaaacaacc tgaaactggg aagctttggc gcatttgata acgaatggca tacgctggct
      181 ttccgctttg ccgggaataa cagccttcag gtgacgccgg ttattgatgg tcaggatggc
      241 acaccgttca cgctgacgca gtcaccggtc agtgcctttg cggcggataa actgcatgtg
      301 acagacatta ccagaggtgc gacttacccg gtactgatag acagcattgc ggtggaagtg
      361 aacagcacag acactgcggc atgataaaaa aaaccgccag cgacaggaat ggacgctggc
      421 ggtggtgata cctatggaga aaaaataaag gaacgatact ttcgtactct ggttttttaa
      481 tgaaaacagt tcttattgtc aacaataacg gaaagaaatt atgacatttc tgaaccagtt
      541 aatgctgtac ttctgtacgg tggtctgtgt gctgtatctc ctttcgggtg ggtacagggc
      601 agtgcgcgat tgctggcgca ggcagattga caaaagggcc gcagagaaaa tcagcgccag
      661 tcagtcagcc ggaagcaaac ccgaagagcc gctcatttag cggcaacttt cttaatcaca
      721 tctttcgacg agaaaatccc atgtcagaaa ttacatccct ggtcactgct gaagcagtga
      781 aggaagtcct gcgctctgaa gaagtccgga gcgcactgaa aca
//
LOCUS       DAZKIP010000377          806 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZKIP010000377 DAZKIP010000000
VERSION     DAZKIP010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.381"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..509)
                     /locus_tag="ACHOUK_005124"
     CDS             complement(<1..509)
                     /locus_tag="ACHOUK_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP9900350.1"
                     /translation="MLYSNILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPEDY
                     FRMSPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALSWWLVCRGEIHKFR
                     CVPHLTGRRFEHGVTDCYTLFRDAYHLAGIDMPDFEREDDWWRNGQNLYLDNMAVTGF
                     YRVPLSSAQA"
     gene            complement(563..>806)
                     /locus_tag="ACHOUK_005125"
     CDS             complement(563..>806)
                     /locus_tag="ACHOUK_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="P22AR C-terminal domain-containing protein"
                     /protein_id="HFP9900351.1"
                     /translation="ANRSQALFRELYPALKQIQSNYSGRCYDYGHEFSYVIGMARDVL
                     INHTRDVDINEPDGPTNLSAWMRLKNKELPPSPHHY"
BASE COUNT          186 a          232 c          201 g          187 t
ORIGIN      
        1 gcctgtgcag aggacagggg cacccggtaa aagccggtga ccgccatatt gtccaggtaa
       61 aggttctgac cgttgcgcca ccagtcatcc tcacgctcaa aatccggcat atcaattccc
      121 gccagatggt aggcatcccg gaacagcgtg taacagtccg tcaccccgtg ctcaaagcgc
      181 cgtcctgtca gatgtggcac acagcggaat ttatgaattt ccccccggca gaccagccac
      241 caggacagtg cactttttat ctgcagccgc cggtcggcct cgctcagcca gggcagacca
      301 ccggggtggc tgtggaccag tgccacaatc tccccctgca tttctgcccg cagccagtcc
      361 tccggcgaca tccggaaata atcttccggc tcaccggaga tattcacgca gggaaaatat
      421 ctttcccctt ccggcgttct caccacgaag ccgcacgact ccgctggtgc acatcgccgg
      481 gcgtgcgcca atatattgct atagagcatg agaactcctg ataaaaaccc agccgaagct
      541 gggtcatttc gttggcaatc tgttagtagt gatgcggtga aggaggtaat tctttattct
      601 taagtctcat ccatgcggaa agattcgttg gtccgtctgg ctcattgata tcaacatctc
      661 gtgtgtgatt aattaaaacg tctctcgcca ttccaataac atacgagaac tcatgaccgt
      721 agtcgtagca tctgccggaa tagttcgatt gaatttgttt tagcgccgga tacagttcgc
      781 ggaataatgc ctgtgagcgg ttggca
//
LOCUS       DAZKIP010000378          784 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZKIP010000378 DAZKIP010000000
VERSION     DAZKIP010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.382"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..>784)
                     /locus_tag="ACHOUK_005126"
     CDS             complement(<1..>784)
                     /locus_tag="ACHOUK_005126"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:1900727 - osmoregulated periplasmic glucan
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFP9900352.1"
                     /translation="QFDKTMMQNIFETNAGEMTSYFNMSVVLWFLFTGILPCGLLLLV
                     NIRYPETWIKGITYRLISMFASLLIIFAIAFFFYKDYASVGRNNSSLNKEIIPTNYIY
                     SGFKYVRDFFVSPGEFRQTGTDASRTINEKQKPVIMFLVVGETARSQNYALNGYSRGT
                     NDFTKKYNELISFHNVQSCGTSTAISVPCMFSDMKRKEFNSRKAVNSENVLDILYRTG
                     VNLLWIENDGGCKGVCKRIPTINIEPSNSDNTLCKKNSCYDEV"
BASE COUNT          257 a          156 c          117 g          254 t
ORIGIN      
        1 aacctcatca taacatgagt ttttcttgca taatgtatta tctgaatttg atggctcaat
       61 attaattgta gggattcgct tacatacacc tttacatccc ccgtcatttt caatccagag
      121 taaatttacc cccgttctgt acagaatatc cagtacattt tccgaattta ccgctttgcg
      181 ggaattgaac tcttttcgct tcatatctga aaacatgcat ggaacagata tggctgtgga
      241 tgtgccacac gactgcacat tatgaaagga tataagttca ttatatttct ttgtaaaatc
      301 attggtacca cgagaatatc cattcagagc atagttttga cttcttgctg tctccccgac
      361 aactaaaaac ataattaccg gcttttgttt ttcatttatt gtgcgggatg catccgttcc
      421 agtctgacga aactcgccag gagaaacaaa aaaatccctt acatatttaa acccactgta
      481 gatataatta gtaggtatta tttctttatt taatgaagag ttgtttctgc caactgatgc
      541 ataatcctta taaaagaaaa acgcaatcgc aaatataatc aagagagatg caaacattga
      601 tattaaacga tatgtaatac cctttatcca tgtctcagga taacggatat ttactaacaa
      661 caatagtcca catggaagta tacccgtaaa taaaaaccac agtacaacag acatattgaa
      721 atatgatgtc atttcacctg catttgtttc aaaaatattc tgcatcattg ttttatcaaa
      781 ctgc
//
LOCUS       DAZKIP010000379          779 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZKIP010000379 DAZKIP010000000
VERSION     DAZKIP010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.383"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..646
                     /gene="ydeO"
                     /locus_tag="ACHOUK_005127"
     CDS             <1..646
                     /gene="ydeO"
                     /locus_tag="ACHOUK_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFP9900353.1"
                     /translation="HFEPYERHFKEIGFNENTIKKYLQCTNIQTVTMPVPAKFLRASN
                     VPTGLLNEMIAYLNSEERNHHNFSELLLFSCLSIFATCKGFITLLTNGVLSVSGKVRN
                     IVNMKLAHPWKLKDICDCLYISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGS
                     VNKIAEQCGYASTSYFIYAFRKHFGNSPKRVSKEYRCQRHTGMNTGNTMNALAI"
BASE COUNT          248 a          150 c          151 g          230 t
ORIGIN      
        1 gcattttgaa ccttatgagc ggcattttaa agaaatcgga tttaatgaaa ataccattaa
       61 aaaatatcta caatgcacta acatccagac agtgacgatg cctgttcctg cgaagttttt
      121 acgtgcttcg aatgtaccga ctggattgct taatgaaatg attgcttatc tcaactcgga
      181 agaacgcaat catcataatt tttcagaact tttgcttttt tcttgcctgt ctatttttgc
      241 cacatgcaaa ggtttcatta cactattaac taacggtgtg ctatccgttt cggggaaagt
      301 gagaaatatt gtcaacatga agctggcgca cccatggaag ctgaaagata tttgtgactg
      361 cctgtacatc agtgaaagcc tgttgaagaa aaaactaaag caagagcaaa cgacattctc
      421 acagattctt ttagatgcca gaatgcagca cgcaaaaaat ttgatacgcg tagaaggttc
      481 agtcaataaa attgccgaac aatgtggtta tgccagtaca tcttatttta tttatgcgtt
      541 ccgcaaacat ttcggcaaca gtccaaagag agtttctaag gagtaccgtt gtcaaaggca
      601 cacgggtatg aatacgggca acacgatgaa tgctttagct atttgattat ttgctaacga
      661 gtagtcaacc acgcacccta cgtaaaaatt aaaaatgggg cagtcattcc ctgccccgcg
      721 ttgtttttag gcgatatatt tattgaaata aataagtgac attcatcaca tatttatgc
//
LOCUS       DAZKIP010000380          765 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZKIP010000380 DAZKIP010000000
VERSION     DAZKIP010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.384"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            30..746
                     /locus_tag="ACHOUK_005128"
     CDS             30..746
                     /locus_tag="ACHOUK_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9900354.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGISEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          255 a          132 c          164 g          214 t
ORIGIN      
        1 gtaaataaat tttcgaggag atcattcctg tgggacgtaa atgggccaat attgttgcta
       61 aaaaaacggc taaagacggt gcaacgtcta aaatttatgc aaaattcggt gtagaaatct
      121 atgctgccgc taaacaaggt gaacccgatc cagaattaaa cacatcttta aaattcgtta
      181 ttgaacgtgc aaagcaggca caagttccaa agcacgttat tgataaagca attgataaag
      241 ccaaaggcgg cggagatgaa acattcgtgc agggccgtta tgaaggcttt ggtcctaatg
      301 gctcaatgat tatcgccgag acattgactt caaatgttaa ccgtacgatt gctaacgttc
      361 gcacaatttt caataaaaaa ggcggcaata tcggagcggc aggttctgtc agctatatgt
      421 ttgacaatac tggtgtgatt gtatttaaag gatcagaccc tgaccatatt tttgaaattt
      481 tacttgaagc tgaagttgat gttcgtgatg tgactgaaga agaaggtaac attgttattt
      541 ataccgaacc tactgacctt cataaaggaa tcgcggctct aaaagcagct ggaatcagtg
      601 agttctcaac aacagaatta gaaatgattg ctcaatctga agttgagctt tcaccagaag
      661 atttagaaat ctttgaaggg cttgttgatg cccttgaaga tgacgacgat gtacaaaaag
      721 tttatcataa cgtcgcaaat ctctaattat attttaaaga aatct
//
LOCUS       DAZKIP010000381          751 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZKIP010000381 DAZKIP010000000
VERSION     DAZKIP010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.385"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..616
                     /locus_tag="ACHOUK_005129"
     CDS             <1..616
                     /locus_tag="ACHOUK_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFP9900355.1"
                     /translation="LRVELEAAKSKLNEQREYYEGVIADGSKRIAELEKQCAEWERKA
                     LSNFEECAAMAERIEEMQTKSAPDSFGIIGENIRTQDNRITSDPMFCVYQKREIAVDA
                     DYDHDRIVWVDEDGNEANKRHSRRLELLHENFREPPEKWRRVAVKDIDEFVTCCFTEQ
                     GCKDYLAVNGHNLRLPFIYVKSGFRNAEYIGIRNWLAGIRIKGE"
     gene            618..>751
                     /locus_tag="ACHOUK_005130"
     CDS             618..>751
                     /locus_tag="ACHOUK_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900356.1"
                     /translation="MALTHRELCQIAYKFLKRNGFKVCFHDRFIAVTSTGEQPDAMGF
                     "
BASE COUNT          229 a          150 c          191 g          181 t
ORIGIN      
        1 gctgcgtgtt gagcttgaag cagcaaaatc aaaactcaac gagcagcgtg aatattacga
       61 gggagtaatc gcggatggaa gtaagcgcat agcagaactg gaaaaacaat gcgccgaatg
      121 ggagcgaaaa gcattaagca actttgaaga gtgtgctgcg atggctgaac gtatcgaaga
      181 gatgcagaca aaatctgcac cagattcgtt tggcatcatc ggtgaaaata ttcgaacaca
      241 ggacaatcga ataacgtcag atcccatgtt ttgtgtgtat caaaagcgcg aaatcgctgt
      301 tgatgctgat tatgaccatg accggattgt ctgggttgac gaagatggca atgaagccaa
      361 taaacgccat agtcgtcgtc tcgagctact tcatgaaaac tttcgagagc caccagaaaa
      421 atggcggcgc gttgctgtga aagatattga tgaattcgtt acctgctgtt tcaccgaaca
      481 gggttgtaaa gactacctgg cagtcaatgg tcacaatctt cgcttgccat ttatatatgt
      541 aaaaagcggt ttcaggaacg ctgaatatat cggcataaga aactggcttg ctggcattcg
      601 catcaaagga gagtgatatg gcgttaacac accgcgaact ctgtcagatt gcgtacaagt
      661 tccttaagcg caacggtttc aaggtttgtt ttcatgaccg ctttatagct gtaaccagta
      721 ccggagaaca gccagatgct atgggattca g
//
LOCUS       DAZKIP010000382          749 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZKIP010000382 DAZKIP010000000
VERSION     DAZKIP010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.386"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>749
                     /locus_tag="ACHOUK_005131"
     CDS             <1..>749
                     /locus_tag="ACHOUK_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900357.1"
                     /translation="FGVLAKNQNTRDIRNIKNFSSHQHSLGDMFDELINIIDKEQVLS
                     TEQRKVIFRRYEDLYVKLMHYSVFTDKTHQIIKQKYFNDIVPMILALDIRNTYHPDNE
                     MAFYYHIHSFLTQIPDNEDDIYHAARTYLRNYVKLCLSGYTPANAHFKDIFDGVYEFI
                     RNIRKNSTPGKTKLIATINTCKETCKHLLYLSNEDKEKIISDLDKVQVACYYLTILLA
                     FERRTSLTSTLATLYKMLISEREVSEYECQL"
BASE COUNT          281 a          131 c          116 g          221 t
ORIGIN      
        1 tttggtgttc tggctaaaaa ccagaatacc cgtgacatcc gaaatattaa aaatttttcc
       61 tcacatcaac attcactggg cgacatgttt gatgaattaa taaacattat tgataaagaa
      121 caagtattga gtacagaaca acgaaaagtt atatttagga gatatgaaga tctctatgtt
      181 aagctaatgc actattctgt ttttacagac aaaacacatc aaataataaa acaaaaatat
      241 tttaatgaca ttgtaccaat gattctcgca ctcgacatca ggaacacata tcacccggat
      301 aatgagatgg cattttacta tcatattcat tcttttctca ctcagatacc ggataatgag
      361 gatgatatat atcatgctgc aaggacatat ctgcgaaatt acgttaagtt atgtttatcc
      421 ggatacacac cagcgaatgc gcatttcaaa gatatctttg atggcgtata tgaattcatt
      481 cgtaatattc gcaaaaacag tacaccagga aaaacaaaac ttatcgcaac tatcaacaca
      541 tgcaaagaaa cctgtaaaca tctgctttat ttaagtaatg aagacaagga aaaaataatt
      601 tctgacttag ataaagttca ggttgcatgt tattatctca ctatattact ggctttcgaa
      661 agacgaactt cattaacaag caccctggca actttatata aaatgctgat aagcgaaaga
      721 gaagtttcag aatatgaatg ccagttatt
//
LOCUS       DAZKIP010000383          743 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZKIP010000383 DAZKIP010000000
VERSION     DAZKIP010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.387"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            61..743
                     /locus_tag="ACHOUK_005132"
                     /pseudo
     CDS             61..743
                     /locus_tag="ACHOUK_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551287.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
BASE COUNT          207 a          144 c          189 g          203 t
ORIGIN      
        1 ttttggataa cggcacttat tgatatattc atgaagatta taatcacaag ggaatacata
       61 atgaaaagaa aagttctggc aatgctggtc ccggcgttat tagttgctgg cgcagcaaat
      121 gcggctgaaa tttataataa agatggcaat aaactggatt tgtacggaaa agtagcgggc
      181 ctgcactact tctctgatga tgctagcagc gatggcgaca tgtcatatgc ccgtatcggt
      241 ttcaaaggtg aaactcagat cgctgaccaa ttcactggtt atggtcagtg ggaatttaac
      301 attggcgcaa acggtcctga aagcgacaag ggtaataccg caacgcgtct ggcatttgca
      361 ggtttcggct ttggtcagaa tggtactttc gactatggtc gtaactacgg tgtcgtatat
      421 gacgtagaag catggaccga tatgctgcca gaatttggtg gagataccta tgctggcgct
      481 gacaacttca tgaacggtcg tgctaacagc gtagcaacct atcgtaacaa tggtttcttt
      541 ggtcaagttg atggtctgaa ctttgcactc cagtatcagg gtaacaacga gaaaagcgga
      601 ttatttgatc aagaaggttc aggtaacggt aatggacgta aacttgctaa agagaacggc
      661 gacggttcag tatgtccact tcctatgact ttgactttgg tttaagtctg ggcgctgctt
      721 actctaactc tgaccgtacc gac
//
LOCUS       DAZKIP010000384          741 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZKIP010000384 DAZKIP010000000
VERSION     DAZKIP010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.388"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..726
                     /gene="escU"
                     /locus_tag="ACHOUK_005133"
     CDS             <1..726
                     /gene="escU"
                     /locus_tag="ACHOUK_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     switch protein EscU"
                     /protein_id="HFP9900358.1"
                     /translation="IGFIFAVEKIKPSAQKISVKNNLKNIFSVKSIFELLKSVFKLVI
                     IVLIFYFMGHSYANEFANFTGLNAYQALVVVAFFVFLLWKGVLFGYLLFSVFDFWFQK
                     HEGLKKMKMSKDEVKREAKDTDGNPEIKGERRRLHSEIQSGSLANNIKKSTVIVKNPT
                     HIAICLYYKLGETPLPLVIETGKDAKALQIIKLAELYDIPVIEDIPLARTLYKNIHKG
                     QYITEDFFEPVAQLIRIAIDLDY"
BASE COUNT          245 a          118 c          140 g          238 t
ORIGIN      
        1 ataggattca tctttgcggt tgaaaaaata aaaccatcgg ctcagaagat tagtgtaaaa
       61 aataacctga aaaatatttt ttctgtaaag agcatttttg agctacttaa atcagtattt
      121 aagttagtga taattgttct cattttttat tttatggggc attcatatgc aaatgagttt
      181 gctaatttca caggactgaa cgcatatcaa gctcttgtcg ttgttgcctt ttttgttttt
      241 cttttatgga aaggcgtgct attcggatat ctactctttt cagtatttga tttctggttc
      301 cagaagcatg agggactgaa gaaaatgaaa atgagtaaag atgaggtgaa acgagaagcc
      361 aaggatactg atggtaaccc tgaaattaaa ggggagcgcc gtcgccttca ttccgagata
      421 caaagtggaa gtttggctaa taacatcaaa aaatcaaccg ttattgttaa aaacccgact
      481 cacattgcga tttgcctata ctataaactt ggggagactc cattaccttt agttattgaa
      541 acaggaaaag atgccaaagc tctacagatc attaaactgg ctgaactcta tgatattcca
      601 gtgattgaag atattccttt agcaagaact ctctataaga atatacataa aggacaatat
      661 ataacagaag acttttttga acctgtggca caattgattc gtattgcgat agaccttgat
      721 tattaatcga taatttgctc a
//
LOCUS       DAZKIP010000385          736 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZKIP010000385 DAZKIP010000000
VERSION     DAZKIP010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.390"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>736
                     /locus_tag="ACHOUK_005134"
     CDS             <1..>736
                     /locus_tag="ACHOUK_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YopT-type cysteine protease domain-containing
                     protein"
                     /protein_id="HFP9900359.1"
                     /translation="PSITTIIKKTEYYGHQLTNAMTVASVVKPYDFSNLGAINSIDKS
                     VSDVPALHTIVEQAKYNLLSWNDFYNTHASIWDTIARQHKSTNIEFHPQSLLFDRDSK
                     GKCLGLSLLYLDTGGYGGGYQKLRHNIDTASTLYQTKYNDNLKLSNRDDFFLRKTQRI
                     ITMSNELGNNRLKNAQLEVLELKDPILTEGILYQRRISSLLITTEYHSLALQQISSFW
                     RVTDPNFGHCDFHSLAQALTFIKNITS"
BASE COUNT          267 a          128 c          119 g          222 t
ORIGIN      
        1 cccagcataa caacaataat aaaaaaaaca gaatattatg ggcatcagtt aacaaatgct
       61 atgactgttg cttcagtggt taaaccatat gactttagca atcttggtgc cataaatagt
      121 atagataaat ccgtttctga tgtgcctgca ttgcatacaa ttgttgaaca agcaaaatat
      181 aatttacttt catggaatga tttttataac actcatgcgt caatatggga tacaatagca
      241 agacagcata aaagtacaaa tatcgaattt cacccacaaa gtcttctttt tgatagagat
      301 tcgaaaggaa agtgccttgg tttatctctt ctgtatcttg atacaggggg gtacggaggc
      361 ggatatcaaa aactgaggca caatattgac actgccagca cattatatca aacaaaatac
      421 aatgacaatc taaaattatc taaccgtgat gatttctttt taagaaaaac acagcgtatc
      481 attacaatgt ccaatgagtt aggtaataac aggttaaaaa atgcacagtt agaggttctg
      541 gaattgaaag accctatact aactgaaggc atattatacc agcgtcgtat atcatctctt
      601 ttaataacaa cagaatatca ttcacttgca ttacaacaaa tatcttcttt ttggagagta
      661 actgatccta attttggtca ttgtgacttt cattccttag cacaggcttt aacgtttata
      721 aaaaatataa cgagca
//
LOCUS       DAZKIP010000386          730 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZKIP010000386 DAZKIP010000000
VERSION     DAZKIP010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.391"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
BASE COUNT          241 a          152 c          140 g          197 t
ORIGIN      
        1 actttaataa ttgcatttcc atttagttaa gcgtttctct ttataagata gatctcattg
       61 cataaacaaa taaaacagaa taaaaaccaa gctgaaccgt ttcactatac tctacttaat
      121 tcgagttaca ggaaggcagc aagcgagtga atccccagga gcttacataa gtaagtgatt
      181 ggggtaagcg aacgcagtcg cagcacatgc aacttgaagt atgacgagta tatatatcta
      241 ttagctgttt cccggacata agctacagcc agcactaacc tggaacgggt gcattataaa
      301 aaggaacaag ggacaaatag ctgaacagta accgcgttag tctcttcccg cataaccgta
      361 ccattcgtat taagaagtat tgcccatatc cattcggcga aagaggatct gccatacatc
      421 aagtccgttt tacttggcat cagatcatcg ccgtcattaa ctctgttaaa gtcggacaaa
      481 cgattagcaa gatctgtgat tcaaaagtgc ttagcgctaa ggtgggactg gttacagatt
      541 cattaaccct gttctttgtc gcacctatcg atcggtgtaa tgccacaaca ggtttatgtt
      601 aatcagaaca atgcgcccct ctataaagag aattctttga taaggcaact tgaaaaggga
      661 aagctaactg atatcaatgt accactttag taatcatcac attatataca ggccaaaata
      721 agtttcctga
//
LOCUS       DAZKIP010000387          723 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZKIP010000387 DAZKIP010000000
VERSION     DAZKIP010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.392"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..255
                     /locus_tag="ACHOUK_005135"
     CDS             <1..255
                     /locus_tag="ACHOUK_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFP9900360.1"
                     /translation="LGENGLYLHGTPYNVAPAEQTHVPLITWMSPGFVSSKKIDLNCL
                     SEHALNRTVSHDNIFSSLLGLWNVNTSVYNKEDDIISSCR"
     gene            complement(348..707)
                     /locus_tag="ACHOUK_005136"
     CDS             complement(348..707)
                     /locus_tag="ACHOUK_005136"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFP9900361.1"
                     /translation="MANTTTGVTRIIKAAGYSWKGLCAAWRYEAAFRQECILVLSALI
                     ISFWLDVDSVTRVLLIGSAMMVIIVEIINSAIEAVVDRIGSEHHELSGRAKDMGSAAV
                     LITILVAIIIWGVLLLS"
BASE COUNT          235 a          144 c          125 g          219 t
ORIGIN      
        1 ctgggagaaa atggattata cttacatgga acaccatata atgttgctcc agctgagcaa
       61 acgcatgtgc ctcttattac ctggatgtct ccggggttcg tttcctctaa aaaaattgat
      121 ttgaattgtt tgtcagaaca tgctctaaac agaacagtaa gtcatgataa tatattttca
      181 tcgttactgg ggttatggaa tgtaaatact tcagtttata ataaagaaga tgatattata
      241 tcctcctgca gatagatact gacatttttc tgaggataag aggtgaatac ctcttatcct
      301 taagtaaata ttaaccaggt aactgtgaat aatttagatg ttgggcgtta tgaaagtaat
      361 aaaactcccc atataatgat agcaactaaa atcgtaatca acactgcggc tgaacccata
      421 tcttttgctc tgcctgataa ttcgtgatgt tctgaaccaa ttctgtcaac aactgcttca
      481 attgcactgt taattatttc aacaataatt accatcattg ccgaaccaat gagtaatact
      541 cgagtaacag agtcaacatc cagccaaaag gatattatca gtgctgacag aaccagaatg
      601 cactcctgac gaaaggctgc ttcatagcgc catgccgcac ataagccttt ccatgaataa
      661 cccgctgctt taataattct ggtaacacca gtggtcgtat tagccataaa tacctcttat
      721 tta
//
LOCUS       DAZKIP010000388          711 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZKIP010000388 DAZKIP010000000
VERSION     DAZKIP010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.393"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            280..587
                     /locus_tag="ACHOUK_005137"
                     /pseudo
     CDS             280..587
                     /locus_tag="ACHOUK_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          231 a          156 c          143 g          181 t
ORIGIN      
        1 aacaaaaccc cttttaatcc cttatatatc aggctttctt tgtgtacagg ggtcaaccga
       61 ctcaacctgt atcaagttgc ttttgaggat actttagggg gtattatttc ctcttgaaat
      121 attagcaccc cataatggga aaaaggtaat acataacaat ggtttttaac agtatctttg
      181 ttatacaagg gggcattgag gatatcagga aaaatctcaa aatcggtacg gacaggaaac
      241 tacgtgggca gcaaaaacgt atggtaacac cgggaaatgc aacgcccacc agtcgggcta
      301 atccaccact ccaatcgtgg ttcgcagtac tgcacacacg attaccgggc tatacaggag
      361 cggtttggtc tgaaaacatc catgccgcgt aaaggtaacc gttacgacaa cacctcaatg
      421 aaaaacttat agtaaacgct gaagaatgaa actatcgttt taataaccgg gatgaagcca
      481 tctaagtaat acggaaatac attgagattt tctacaacca tcagcgccgc cactcccgtc
      541 tggggaatat ctccctggta gccttcagga aaaatataac cagataactg cgtaaaaaaa
      601 gaacaaatgg tggtgtccgc tattgccagt atacctgagt ttactgcctt tcagattatc
      661 acaatcaata gcgaacagac ttttaagctc tgtgcgcttt taacaaaacg t
//
LOCUS       DAZKIP010000389          706 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZKIP010000389 DAZKIP010000000
VERSION     DAZKIP010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.394"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..81
                     /locus_tag="ACHOUK_005138"
     CDS             <1..81
                     /locus_tag="ACHOUK_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900362.1"
                     /translation="YPLMKVISAQQAISALKYMVDGEVYF"
     gene            488..586
                     /locus_tag="ACHOUK_005139"
     CDS             488..586
                     /locus_tag="ACHOUK_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004141699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900363.1"
                     /translation="MRPQSELLTLSQMQKCTCDVLHPAFDLCGGEA"
     gene            583..>706
                     /locus_tag="ACHOUK_005140"
     CDS             583..>706
                     /locus_tag="ACHOUK_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900364.1"
                     /translation="MNLPQDGIKLHRGNFTAVGQQIQTYLEDGKCFRMVLKPWRE"
BASE COUNT          208 a          155 c          148 g          195 t
ORIGIN      
        1 tatcctttaa tgaaagtaat atccgcccag caggcgataa gtgctctcaa atatatggtt
       61 gatggtgagg tttatttttg agtaataatc actttttcaa ccgtgctatg gtatgaaagt
      121 aaagtaacta ctatgatatt aactatcgag caagaatatc cccaggcact caacagggcg
      181 caggtcaacg tgaatcggtc atgacacgac accgattttg cggaatcaat gttgcttatg
      241 aatacaacat cccgcttagt acatgttcaa cacatcagca aattttgaac tgggtgtggc
      301 acttaactga aaaaacatgg atgacacaaa atgttactcg tcgcttcatt gaagtagctt
      361 gtggatatca caagctagac tatcgccaat gatattcaat ttttgatttt ttagtatcaa
      421 ccttccataa taatgtcacc ggagcttgaa caactccggt gacttctgcg catttaaggg
      481 gacttaaatg cgaccacaat ctgaactcct taccttgtca cagatgcaga aatgcacctg
      541 cgatgtcttg catccagcgt ttgatctctg cggaggtgaa gcgtgaacct cccacaagat
      601 ggcatcaaat tacatcgcgg taacttcacc gctgtcggtc agcagatcca gacttatctg
      661 gaagacggca aatgctttcg catggtgctt aaaccgtggc gcgaga
//
LOCUS       DAZKIP010000390          702 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZKIP010000390 DAZKIP010000000
VERSION     DAZKIP010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.395"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..310
                     /gene="ybcN"
                     /locus_tag="ACHOUK_005141"
     CDS             <1..310
                     /gene="ybcN"
                     /locus_tag="ACHOUK_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HFP9900365.1"
                     /translation="LSHMWYSEISEYLISRGKSFATSAWVKDALKHTYLGYETKDLVD
                     VVTGEITTIQSLRHTSDLGTGEMYVFLCKVEAWAMNIGCHLTIPQSCEFQLLRDKQEA
                     "
     gene            310..480
                     /locus_tag="ACHOUK_005142"
     CDS             310..480
                     /locus_tag="ACHOUK_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFP9900366.1"
                     /translation="MATPLIRVMNGHIYKVPNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            473..>702
                     /locus_tag="ACHOUK_005143"
     CDS             473..>702
                     /locus_tag="ACHOUK_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFP9900367.1"
                     /translation="MADLRKAARSRECQVRIPGVCNGNPETSVLAHIRLTGLCGTGTK
                     PPDLIATIACSACHDEIDRRTHFVDAAYAKECA"
BASE COUNT          194 a          180 c          174 g          154 t
ORIGIN      
        1 actcagccac atgtggtaca gcgaaatcag tgaatacctc atcagcaggg ggaaatcgtt
       61 cgctacttca gcatgggtaa aagatgctct caaacacaca tacctcggtt atgaaaccaa
      121 ggacctggtt gatgtcgtaa ccggcgaaat cactactatc cagtcgttac gccatacctc
      181 cgatcttggt accggagaga tgtatgtctt cctgtgtaag gttgaagcct gggcgatgaa
      241 tattggctgc cacctgacta ttccacagag ctgcgagttc cagctgctgc gcgacaagca
      301 ggaggcgtaa tggctacacc gcttattcgt gtcatgaacg gacacatcta caaagtacca
      361 aatcgtcgta agcgtaaacc tgagctgaag ccatccgaaa taccaacact gctcggatat
      421 accgccagcc tggttgataa aaaatggttg cgactggcag caaggaggaa tcatggctga
      481 tttgagaaaa gcagcgcgta gtcgggaatg ccaagtaaga atccctggcg tatgtaatgg
      541 caaccctgaa acgtctgtac tggcacatat ccggctgact ggattgtgcg gcaccggtac
      601 caaaccgcca gacttgattg ccaccattgc atgttctgcc tgccacgacg aaatcgaccg
      661 ccgcacacat tttgtcgatg ctgcatatgc aaaagaatgc gc
//
LOCUS       DAZKIP010000391          691 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZKIP010000391 DAZKIP010000000
VERSION     DAZKIP010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.396"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..479
                     /locus_tag="ACHOUK_005144"
     CDS             <1..479
                     /locus_tag="ACHOUK_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFP9900368.1"
                     /translation="DNFPEVSEANILSTFEQLHQNKDEVFERGVINVFRGLSWNYKTN
                     CPCKFGSKIIVNNLVRWDRWGFHLITGHQVDRLADLERMLHLFSGKPIPDNRENITLR
                     LDDHIQSVQGKEDYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPDMLAV"
BASE COUNT          210 a          152 c          171 g          158 t
ORIGIN      
        1 acgataattt tccggaagtc agtgaagcga acattctgag cacatttgaa caactgcacc
       61 agaacaaaga tgaagtgttt gagcgagggg tgatcaacgt cttcagggga ttgagctgga
      121 attacaaaac taattgcccc tgtaaatttg gcagtaaaat tatcgtcaac aatctggtga
      181 gatgggaccg gtggggattt catcttatca ccgggcacca ggtagatcga cttgccgacc
      241 tggaaagaat gctgcacctg ttcagcggca aaccgatccc cgacaaccgg gaaaacatca
      301 cccttcgtct ggatgaccac atccagtctg ttcagggtaa agaggactat gaagatgaga
      361 tgttcagcat cagatacttt aagaagggct ccgcacacat cacgttcagg aaaccagaac
      421 tggttgacag actgaatgat attatcgcaa aacactatcc tgacatgctg gcagtatagc
      481 tttgccagta tgctcaaaga tcggaaattg tccgataaga tatgatcgcg gggcatataa
      541 accggtttca gatggcatac ccgatggcat actgtccgga aggataaata aaaaagcgca
      601 tgttaaacaa taacatgcgc ttataatctg gcggtgaggg ggggattcga acccccgata
      661 cgttgccgta tacacacttt ccaggcgtgc t
//
LOCUS       DAZKIP010000392          681 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZKIP010000392 DAZKIP010000000
VERSION     DAZKIP010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.397"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(25..636)
                     /gene="map"
                     /locus_tag="ACHOUK_005145"
     CDS             complement(25..636)
                     /gene="map"
                     /locus_tag="ACHOUK_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector Map (Rho
                     guanine exchange factor)"
                     /protein_id="HFP9900369.1"
                     /translation="MFSPMTMAGRSLVQATAQTLKPAVTRAAMQAGTGATGMRFMPVQ
                     SNFVINHGKLTNQLLQAVAKQTRNGDTQQWFQQEQTTYISRTVNRTLDDYCRSNNSVI
                     SKETKGHIFRAVENALQQPLDMNGAQSSIGHFLQSNKYFNQKVDEQCGKRVDPITRFN
                     TQTKMIEQVSQEIFERNFSGFKVSEIKAITQNAILEHVQDTRL"
BASE COUNT          173 a          154 c          130 g          224 t
ORIGIN      
        1 aatttatacc tgacaaggtt atccctacaa tcgggtatcc tgtacatgct ctaaaatcgc
       61 attctgagta atcgctttta tctcactgac tttgaagccg ctaaagttgc gttcaaatat
      121 ttcttgtgag acttgttcta tcatcttcgt ctgggtgtta aaacgtgtta ttggatccac
      181 tcttttgccg cactgctcat caactttctg attaaagtat ttattggact gcaagaagtg
      241 accaattgat gattgtgccc cgttcatatc aagaggttgc tgtaacgcat tttcaacagc
      301 tctaaaaatg tgacctttag tctccttact aatgaccgaa ttattactcc tgcaatagtc
      361 atccagagtt ctatttactg ttctggatat ataagtcgtc tgctcctgct ggaaccattg
      421 ttgggtatcg ccattacgag tttgtttggc tacagcctgc aggagttggt tagtcagttt
      481 gccatgatta atcacaaagt tcgactgcac gggcatgaac ctcatacccg tggctcccgt
      541 acccgcttgc attgcagctc tggtaaccgc aggtttaagc gtctgtgcag tcgcctgaac
      601 taacgatctg cctgccattg tcattggact aaacatacca taaaccttta taaattatat
      661 ttcgacagta aaaaaattgc t
//
LOCUS       DAZKIP010000393          678 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZKIP010000393 DAZKIP010000000
VERSION     DAZKIP010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.398"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            1..186
                     /locus_tag="ACHOUK_005146"
     CDS             1..186
                     /locus_tag="ACHOUK_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP9900370.1"
                     /translation="MNTAAEAIRALSLQMPGFRRQMNEGWYQTRIPTASQNRSPAPVF
                     VVMSGEYLLDKKEEIIQ"
     gene            199..288
                     /locus_tag="ACHOUK_005147"
     CDS             199..288
                     /locus_tag="ACHOUK_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900371.1"
                     /translation="MPINLTSYLWVQGQKVVPAVVFSNFTLVD"
     gene            308..>678
                     /locus_tag="ACHOUK_005148"
     CDS             308..>678
                     /locus_tag="ACHOUK_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="secreted by salmonella pathogenicity island 1;
                     involved in survival of Salmonella in host cells;
                     interferes with host cell ubiquination pathway; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HFP9900372.1"
                     /translation="MLPTTNISVNSGVISFESPVDSPSNEDVEVALEKWCAEGEFSEN
                     RHEVASKILDVISTNGETLSISEPITTLPDLLPGSLKELVLNGCTELKSINCLPPNLS
                     SLSMVGCSSLEVINCSIPENV"
BASE COUNT          206 a          125 c          155 g          192 t
ORIGIN      
        1 gtgaacacgg cagcggaagc catccgtgcc ctgtcgctgc agatgccggg attccgccgt
       61 cagatgaacg aaggctggta ccagacacgt attccgacag cttcacaaaa ccggagtccg
      121 gctccggttt ttgttgtcat gtccggggaa tatttgttag ataaaaaaga ggagataatt
      181 caatagggag ttaaattaat gccgataaat ctgacatctt atttgtgggt acagggacag
      241 aaagttgtcc cggcagttgt gttttctaat tttactttag tggactaagt aaaaaggagt
      301 gagataaatg ctgcccacta caaatatctc tgtaaattct ggagtaatat cttttgaaag
      361 tcctgtagat tcaccatcta acgaggatgt tgaagttgcc ctcgaaaagt ggtgtgctga
      421 gggagaattt agcgaaaatc gtcatgaggt tgcatcaaaa atacttgatg ttataagtac
      481 taatggagag actttatcaa tcagtgagcc aataacaaca ttaccagact tgcttccagg
      541 ttctctgaaa gaactggttt tgaatggatg tacagagctt aaatcaataa actgcttacc
      601 ccccaactta tcttcattaa gtatggttgg atgctcatca ttagaggtta taaattgcag
      661 catacctgaa aatgtcat
//
LOCUS       DAZKIP010000394          659 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZKIP010000394 DAZKIP010000000
VERSION     DAZKIP010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.399"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            14..>659
                     /gene="wzx"
                     /locus_tag="ACHOUK_005149"
     CDS             14..>659
                     /gene="wzx"
                     /locus_tag="ACHOUK_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O157 family O-antigen flippase"
                     /protein_id="HFP9900373.1"
                     /translation="MIMNKIKKILKFCTLKKYDTSSALGREQERYRIISLSVISSLIS
                     KILSLLSLILTVSLTLPYLGQERFGVWMTITSLGAALTFLDLGIGNALTNRIAHSFAC
                     GKNLKMSRQISGGLTLLAGLSFVITAICYITSGMIDWQLVIKGINENVYAELQHSIKV
                     FVIIFGLGIYSNGVQKVYMGIQKAYISNIVNAIFILLSIITLVISSKLHAGLPVL"
BASE COUNT          223 a           90 c          119 g          227 t
ORIGIN      
        1 aatagttcac catatgataa tgaataaaat caaaaaaata cttaaatttt gcactttaaa
       61 aaaatatgat acatcaagtg ctttaggtag agaacaggaa aggtacagga ttatatcctt
      121 gtctgttatt tcaagtttga ttagtaaaat actctcacta ctttctctta tattaactgt
      181 aagtttaact ttaccttatt taggacaaga gagatttggt gtatggatga ctattaccag
      241 tcttggtgct gctctgacat ttttggactt aggtatagga aatgcattaa caaacaggat
      301 cgcacattca tttgcgtgtg gcaaaaattt aaagatgagt cggcaaatta gtggtgggct
      361 cactttgctg gctggattat cgtttgtcat aactgcaata tgctatatta cttctggcat
      421 gattgattgg caactagtaa taaaaggtat aaacgagaat gtgtatgcag agttacaaca
      481 ctcaattaaa gtctttgtaa tcatatttgg acttggaatt tattcaaatg gtgtgcaaaa
      541 agtttatatg ggaatacaaa aagcctatat aagtaatatt gttaatgcca tatttatatt
      601 gttatctatt attactctag taatatcgtc gaaactacat gcgggactac cagttttaa
//
LOCUS       DAZKIP010000395          659 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZKIP010000395 DAZKIP010000000
VERSION     DAZKIP010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.400"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..>659)
                     /gene="sepL"
                     /locus_tag="ACHOUK_005150"
     CDS             complement(<1..>659)
                     /gene="sepL"
                     /locus_tag="ACHOUK_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE gatekeeper SepL"
                     /protein_id="HFP9900374.1"
                     /translation="SETIEGLSLGYRKGSARKEEEGSTIEKLLNDMQELLTLTDSDKI
                     KELSLKNSGLLEQHDPTLAMFGNMPKGEIVALISSLLQSKFVKIELKKKYARLLLDLL
                     GEDDWELALLSWLGVGELNQEGIQKIKKLYEKAKDEDSENGASLLDWFMEIKDLPERE
                     KHLKVIIRALSFDLSYMSSFEDKVKTSSIISDLCRVIIFLSLDNYADIISISIKKDKD
                     I"
BASE COUNT          195 a          153 c           99 g          212 t
ORIGIN      
        1 atgatatctt tatctttctt aatagagatc gaaataatat ctgcatagtt atcaagtgat
       61 aaaaaaatga ttaccctgca taaatcacta ataattgaag atgtctttac tttgtcttca
      121 aaagaagaca tataagagag atcgaacgac agcgccctaa taatgacttt taagtgcttc
      181 tcacgctcgg gaagatcctt aatctccata aaccagtcaa gtaaagaggc gccattttca
      241 gaatcctcat ccttagcctt ttcataaagc ttcttgatct tctggatacc ttcctgattt
      301 aactcaccca cccctaacca ggaaagcaga gccagctccc aatcatcttc gcctaataaa
      361 tctaataata acctggcata tttctttttt aattcaatct taacaaactt agattgcaat
      421 aaagaagata ttagcgcaac aatttccccc tttggcatgt tgccaaacat cgccaaagta
      481 ggatcgtgtt gttccagcag cccgctgttt tttaatgata gttcttttat tttatcacta
      541 tcggtaagag taagcagctc ttgcatatca ttgagcagct tctcgattgt cgagccttcc
      601 tcttctttcc ttgcgctacc tttgcgatat cccaggctta gcccttcaat cgtttcaga
//
LOCUS       DAZKIP010000396          634 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZKIP010000396 DAZKIP010000000
VERSION     DAZKIP010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.401"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            3..581
                     /locus_tag="ACHOUK_005151"
     CDS             3..581
                     /locus_tag="ACHOUK_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFP9900375.1"
                     /translation="MIGFLGHYPDLYEVVGGYKDLKKAIAECNKSAAQIFILGEFAGG
                     MMGAELVKWVKSHKIDAHIITFVAKMPYIDSIKLLEAGAKGCVWKTSHPAKLNRAIDS
                     ISNGYTYFDSVHMDCEKISSRYSSDNQLTNRESEILQLIADGKTNKEIANFLQLSRKT
                     VETHRLNIMKKLDVHSGIELIKTALRMGVCTI"
BASE COUNT          217 a           99 c          134 g          184 t
ORIGIN      
        1 ggataattgg attcctcgga cattatcccg atttatatga ggttgttggg ggctataaag
       61 atctgaagaa agctatagcg gagtgtaaca aatctgcagc ccaaatattt attttaggag
      121 aatttgcagg tggaatgatg ggggccgaac tcgtaaaatg ggttaaatcg cataaaatag
      181 atgctcatat tattacattt gtagcaaaaa tgccgtatat tgattcaata aaattgcttg
      241 aagcaggtgc gaaaggatgc gtatggaaaa ccagtcaccc ggcgaaactt aatcgcgcta
      301 ttgattcgat tagtaacggc tacacttatt ttgatagtgt acatatggat tgtgagaaaa
      361 tatcttccag atattcctct gataatcaac ttacaaatcg tgagtctgaa atattacaac
      421 tcatagctga tggaaaaaca aacaaagaaa tcgctaattt cttgcagtta agcaggaaaa
      481 cagtcgaaac tcatagactc aatatcatga agaaattaga tgttcacagt ggtatcgagt
      541 tgatcaaaac agctttacgt atgggtgtgt gcactatata aaaaaaagag gctttaggct
      601 cattttgcta aagccttacg attgtctagt tcat
//
LOCUS       DAZKIP010000397          624 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZKIP010000397 DAZKIP010000000
VERSION     DAZKIP010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.402"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            262..>624
                     /gene="tccP"
                     /locus_tag="ACHOUK_005152"
                     /gene_synonym="espF(U)"
     CDS             262..>624
                     /gene="tccP"
                     /locus_tag="ACHOUK_005152"
                     /gene_synonym="espF(U)"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tir-cytoskeleton coupling protein TccP"
                     /protein_id="HFP9900376.1"
                     /translation="MINNVSSLFPTVNRNITAVYKKSSFSVSPQKITLNPVKISSPFS
                     PSSSSISATTLFRAPNAHSASFHRQSTAESSLHQQLPNVRQRLIQHLAEHGIKPARSM
                     AEHIPPAPNWPAPPPPVQN"
BASE COUNT          195 a          149 c          105 g          175 t
ORIGIN      
        1 gtagattttt ggcattttgg gaagatcagc gggacataag agacatacaa cacaggagga
       61 atggctgttt tttaatagtg ctttgaattc cgcatgaaac ctttcttagc agtcgaaaag
      121 caaaacatac gttttctttt taacaataat tgatgagtat tgcatcgagt gtcatttata
      181 catcgccgca tttatttgcc actataaaaa ggctgattac atatctatcg gagaactaaa
      241 attacttata aggtaatctg catgattaac aatgtttctt cactttttcc aaccgtcaac
      301 cgcaatatta cagctgtata taaaaaaagc agcttctctg tatcaccaca gaaaatcaca
      361 ttaaatcctg taaaaatcag ctcacctttt tcaccaagca gtagctccat cagcgcaaca
      421 actctctttc gagccccaaa cgcccattcg gcatcatttc atcgacagtc tactgctgaa
      481 agttcgttac atcaacaact tcctaatgtg aggcagcgcc tgatacaaca tcttgcagag
      541 catggcatta aacctgcccg gagtatggct gaacatattc ctccggcacc taactggcct
      601 gcgccaccac cgccagtaca aaat
//
LOCUS       DAZKIP010000398          624 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZKIP010000398 DAZKIP010000000
VERSION     DAZKIP010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.403"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..589)
                     /locus_tag="ACHOUK_005153"
     CDS             complement(<1..589)
                     /locus_tag="ACHOUK_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HFP9900377.1"
                     /translation="MDTPDEKERITFPQVSRRSFLQATSALITLPFISSTAKAQSPDA
                     SPEVTAPVADKVVPTCSTFDCGGKCDIRAHMRDGVVTQITTLPDNELDPQMPIMRACV
                     RGRGYRKFVYHPDRLKYPMKRVGKRGEGKFERISWDEATTLIADNLKRITQQYGPASR
                     YVHVGTAVSGGTFSGDAMARRLLNLTGGYLEYYHSV"
BASE COUNT          172 a          156 c          141 g          155 t
ORIGIN      
        1 taactgagtg ataatattca agataaccac cggttaaatt taacagccga cgcgccatcg
       61 catcgccaga aaatgttcca ccagaaacag ccgtcccgac atggacataa cgtgaagctg
      121 ggccatattg ttgcgtgatc cttttcaggt tatcggcaat taaggtcgtc gcctcatccc
      181 atgagatacg ctcaaattta ccctcgcctc gtttacctac ccgcttcatc ggatatttca
      241 aacggtctgg atgataaaca aatttacgat agccgcgacc acgaacacag gcgcgcataa
      301 tcggcatttg tggatccaat tcgttatcgg gaagcgtggt aatttgcgtc accacgccgt
      361 cgcgcatatg cgcccgaata tcgcacttac caccgcagtc aaatgtgcta caggtgggca
      421 cgactttatc agctactgga gctgtaactt ctggcgacgc atctggggat tgcgcctttg
      481 cggtagaaga aataaaaggc aaagtgataa gcgcggaagt cgcttgtaaa aaactacgtc
      541 gtgatacttg cggaaatgta atgcgttcct tttcgtctgg cgtatccatc ttgcgttccc
      601 gaaaatgaaa aaataaatat tcac
//
LOCUS       DAZKIP010000399          619 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZKIP010000399 DAZKIP010000000
VERSION     DAZKIP010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.404"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>619
                     /locus_tag="ACHOUK_005154"
     CDS             <1..>619
                     /locus_tag="ACHOUK_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFP9900378.1"
                     /translation="LDRLVQQRGFDGRTQRYHYDLTGKLTQSEDEGLVTLWHYDESDR
                     LTHRTVNGEPAEQWQYDEHGWLTEISHLSEGHQVAVHYGYDDKGRLAGERQTVHNPET
                     GELLWQHETEHAYNEQGLANRVTPDSLPRVEWLTYGSGYLAGMKLGGTPLVEFTRDRL
                     HRETVRSFGNNAYELTSTYTPAGHLQSQRLNSQVYDRDYDWNDNG"
BASE COUNT          147 a          177 c          206 g           89 t
ORIGIN      
        1 cgcttgaccg gctggtacag cagcgcggct ttgacgggcg gacgcaacgt taccactatg
       61 acctgaccgg aaaactcacg cagagtgaag atgaggggct tgtcaccctc tggcactacg
      121 acgaatcgga ccgcctcact caccgcacgg tgaacggcga accggcagag cagtggcagt
      181 acgacgagca cggctggctg acagaaatca gccacctgag cgaaggccat caggtggcgg
      241 tgcattacgg ttatgatgat aagggccgcc tggccgggga gcgccagacg gtgcataacc
      301 cggagacggg ggaactgctg tggcagcatg agacagagca cgcatacaac gaacagggtc
      361 tggcaaaccg cgtcacgccg gacagcctgc cgcgggtgga gtggctgacc tacggcagcg
      421 gttatcttgc ggggatgaag ctgggcggga cgccgctggt ggagttcacg cgcgacaggc
      481 tgcaccgcga gacggtgcgc agcttcggca ataacgcata cgaactgacc agcacataca
      541 ctcccgcagg ccatttacag agccagcgcc tgaacagcca ggtgtatgac cgtgactacg
      601 actggaatga caatggcga
//
LOCUS       DAZKIP010000400          616 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZKIP010000400 DAZKIP010000000
VERSION     DAZKIP010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.405"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>616
                     /locus_tag="ACHOUK_005155"
     CDS             <1..>616
                     /locus_tag="ACHOUK_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020240481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-three component system protein"
                     /protein_id="HFP9900379.1"
                     /translation="EIRVTLSDYSRIIIIAHSMGGVISKNLILKKVEHEEQSNIIGFI
                     SLAVPHFGAKLANITSMVSSNAQLVDLGLLSEATDTLNRRWINCSKKLPITRYVYGSH
                     DTIVDKKSALPMDSERNNSIAVNEGHSSICKPENSSSTVFVAVKQFIQQINLEAPKKI
                     CVERFSDEKQYDNEYFVLKMIVADIHQDIAMHAKEYYYNAELARN"
BASE COUNT          212 a           92 c          128 g          184 t
ORIGIN      
        1 tgagattagg gtaacgctaa gtgattactc tcgaattata ataatcgccc atagtatggg
       61 gggggtaata tcaaaaaatc taattctaaa aaaagttgag catgaggaac aatctaatat
      121 aattggattc atttcgttag cagtccctca ctttggtgct aaattggcga atataacttc
      181 aatggtatct tcaaatgctc agttagtaga tcttggattg ttaagtgaag cgaccgatac
      241 gttgaatagg cgctggataa attgtagcaa aaaacttcct atcactagat atgtttatgg
      301 ttctcatgac actatagttg ataaaaaaag cgcattaccg atggatagtg aacgaaataa
      361 ctcaattgca gttaatgaag ggcatagctc aatttgcaag cccgaaaaca gctcgtctac
      421 agtgtttgtt gcggtaaagc aatttattca gcagataaat ttagaagcac ccaagaagat
      481 atgtgttgag cgtttttctg atgagaaaca atatgataat gagtatttcg tcttaaaaat
      541 gattgtcgca gatatacatc aggatatcgc tatgcatgca aaggaatatt attataatgc
      601 tgagcttgcg agaaat
//
LOCUS       DAZKIP010000401          612 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZKIP010000401 DAZKIP010000000
VERSION     DAZKIP010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.406"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            52..>612
                     /locus_tag="ACHOUK_005156"
     CDS             52..>612
                     /locus_tag="ACHOUK_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFP9900380.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASLAQGVEASLRWLTEMTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGA"
BASE COUNT          145 a          155 c          174 g          138 t
ORIGIN      
        1 tgagctcatt actgacagta aggcgctgag ggaagagctg gagacaatgc tgtgacttca
       61 ccatttattc aacaaattgc tgataaccgg gtatgtcagg tactcacctg tcttcctgaa
      121 aaatttgtgg ttgatttcgc aaacggaatc gatgttgcac aggagcatat ccgcacggcc
      181 ggagagcgca cgttcttccg gcgtttaaaa gaggggctga ccggcgaagg cgctgcacgg
      241 cagaatgcca tcaatgcgtc gcttgcacag ggcgttgagg cgtccctgcg ctggctgacg
      301 gagatgacaa cgtcacttgc caccacaaac tacgcgatta cccgggtaaa cgacagggtc
      361 agttcactgg tcagtgatac agccaggctg gcgcattatt ccgcagatac gcgggagcag
      421 ttactcaccc tggccgatca ggttcaccat aaactgaatc atcttgaaga aaaactccac
      481 cgtgttgacc aggttcagcg ggcgcagtta catcttgagc agatattctc atggtggagc
      541 gccgggcgat atgcgtcatt ttcccctgcc ggacgttgtt atgtggcact tgaagagctt
      601 cgctggggcg cg
//
LOCUS       DAZKIP010000402          608 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZKIP010000402 DAZKIP010000000
VERSION     DAZKIP010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.407"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..71
                     /locus_tag="ACHOUK_005157"
     CDS             <1..71
                     /locus_tag="ACHOUK_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFP9900381.1"
                     /translation="RAIKAEYQQKLKKLRNSRSEVA"
     gene            68..>608
                     /locus_tag="ACHOUK_005158"
     CDS             68..>608
                     /locus_tag="ACHOUK_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFP9900382.1"
                     /translation="MNIYERIDGSKYRNIWVVGDLHGCYTNLMKKLETIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NLDYDKEILAKALAHKADELPLIIELVSKGKKYVICHADYPCDKYEFGKPVDHQQVIW
                     NRERISNSQDGIVKEIKGAD"
BASE COUNT          201 a          119 c          153 g          135 t
ORIGIN      
        1 gcagggccat caaggcggag tatcaacaga aacttaaaaa actgcgaaac agcagaagtg
       61 aggttgcatg aatatctacg aaagaattga tggcagcaaa taccgaaata tttgggtagt
      121 tggcgatctg cacggatgct acacgaacct gatgaaaaaa ctggagacga taggattcga
      181 caccaaaaaa gacctgctta tctcggtggg cgatttggtt gatcgcggta cagagaacgt
      241 agaatgcctg gaattaatca cattcccctg gttcagagct gtacgtggaa accatgagca
      301 aatgatgatt gatggcttat cagagcgtgg aaacgtcaat cactggctgc ttaatggcgg
      361 tggctggttc tttaatctcg attacgacaa agaaattctg gctaaagctc ttgcccataa
      421 agcagatgaa cttccgttaa tcatcgaact ggtgagtaaa ggaaaaaaat atgtcatctg
      481 ccacgccgat tatccttgtg ataaatacga gtttggaaag ccagttgatc atcagcaggt
      541 aatctggaac cgcgaacgaa tcagcaactc acaagacggg atcgtgaaag aaatcaaagg
      601 cgcggaca
//
LOCUS       DAZKIP010000403          603 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZKIP010000403 DAZKIP010000000
VERSION     DAZKIP010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.408"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>603
                     /locus_tag="ACHOUK_005159"
     CDS             <1..>603
                     /locus_tag="ACHOUK_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFP9900383.1"
                     /translation="TDLIPEGVIRTDDERTHRYHYDSQHRLVHYTRTQYEEPLVESRY
                     LYDPLGRRVAKRVWRRERDLTGWMSLSRKPQVTWYGWDGDRLTTIQNDRTRIQTIYQP
                     GSFTPLIRVETATGELAKTQRRSLADALQQSGGEDGGSVVFPPVLVQMLDRLESEILA
                     DRVSEESRRWLASCGLTVAQMQSQMDPVYTPARKIHLYHC"
BASE COUNT          136 a          176 c          199 g           92 t
ORIGIN      
        1 aaccgacctc atcccggaag gggttatccg cacggatgat gagcggactc accggtacca
       61 ttacgacagt cagcaccggc tggtgcacta cacgcggaca caatatgaag agccgctggt
      121 cgaaagccgc tatctttacg acccgctggg ccgcagggtg gcaaaacggg tgtggcgacg
      181 tgaacgggac ctgacgggct ggatgtcgct gtcacggaaa ccgcaagtga cctggtacgg
      241 ctgggacggc gaccggctga ccacaataca gaacgacaga acccgcatcc agacgattta
      301 tcagccgggg agcttcacgc cactcatcag ggttgaaacc gccaccggtg agctggcgaa
      361 aacgcagcgc cgcagcctgg cggatgcgct tcagcagtcc ggcggcgaag acggtggcag
      421 tgtggtgttc ccgccggtgc tggtgcagat gctcgaccgg ctggaaagtg aaatcctggc
      481 tgaccgggtg agtgaggaaa gccgccgctg gctggcatcg tgcggcctga ctgtggcgca
      541 gatgcaaagc cagatggacc cggtatacac gccggcgcga aaaatccacc tgtaccactg
      601 cga
//
LOCUS       DAZKIP010000404          593 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZKIP010000404 DAZKIP010000000
VERSION     DAZKIP010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.409"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            37..222
                     /locus_tag="ACHOUK_005160"
     CDS             37..222
                     /locus_tag="ACHOUK_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFP9900384.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWIMQPA
                     PDWQTPLNGIISSSENG"
BASE COUNT          146 a          146 c          163 g          138 t
ORIGIN      
        1 ctgcgctcga tgcaaaatac tcgagggagt tagccgatgc gagagctgaa aatgaaactc
       61 tgcgtgctga tgttgccgct ggtcgtaagc gcctgcggat caacgccacc tgctccggta
      121 ccgtgcgtga agccaccggc acctccggcg tggataatgc aaccggcccc cgactggcag
      181 acaccgctga acgggattat ttcatcctca gagaacggtt gatgacaatg cagaagcagc
      241 tggaaggggc acaggactat atccgcactc agtgcctgaa ctaagttttg ctgatgcgcc
      301 gtatcgtcgc cgtattcctg cattaacaga gaccgcagcc cgacagggag actcctctgc
      361 gagagtgtgc ggggataatc aaaaacgata cacaccgggg tttaccgcgt aaacggagcg
      421 cggcgttctc ccctcatggt cgcccgtccg gtgcgatggt ggaagaaact ggaatctgtt
      481 caataaaaaa actgccgtgt tggagtcaca gcagtaatgt actgattggg tagaagatta
      541 ttattgttat gctttatttt tattctatat ggctgattat ttcaattcgg aat
//
LOCUS       DAZKIP010000405          592 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZKIP010000405 DAZKIP010000000
VERSION     DAZKIP010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.410"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..347
                     /locus_tag="ACHOUK_005161"
     CDS             <1..347
                     /locus_tag="ACHOUK_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HFP9900385.1"
                     /translation="YRERFAERGFFYKVPDVLRDYLSAIPLEINEKARYKPGIANYHN
                     IITCGFSTLLPYIRQQPLAMQQRFNLLFPDFVDHILSPLPLASTLLERITFYAKKNRD
                     ELDKISCKWCCD"
     gene            289..546
                     /locus_tag="ACHOUK_005162"
                     /pseudo
     CDS             289..546
                     /locus_tag="ACHOUK_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842356.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          217 a           90 c          106 g          179 t
ORIGIN      
        1 tttatcgaga aaggtttgca gaacgcgggt ttttttataa ggttcctgat gtactaaggg
       61 attatttatc agctataccg ctggaaataa atgaaaaggc acgatacaaa cccggcatcg
      121 ctaactatca caatatcatc acatgtggtt tttcaacgtt gttaccctat ataaggcaac
      181 aaccattggc aatgcagcaa cgatttaatt tattatttcc tgattttgtt gaccacatac
      241 tatcgccgtt accacttgca tcaacacttt tagagagaat tacattttat gcgaaaaaaa
      301 atagagatga gcttgataaa atctcctgca aatggtgttg tgattaaaag aaaaatttct
      361 gatggattga aagaaatagt ttctcttaaa gaaaaaatcc ttcttgaaac caccgcaaaa
      421 attcagagta ttgaggaaaa gagagaagaa aaatttattc aaggttatta tgatggctat
      481 acaaaaggca ttattgatgt gatggataat tttataccct tgataagctt agctatgctc
      541 agaacttgaa aaaaaagaat aaacatgata aacgatctga agagtatatt gt
//
LOCUS       DAZKIP010000406          590 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZKIP010000406 DAZKIP010000000
VERSION     DAZKIP010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.411"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            123..347
                     /locus_tag="ACHOUK_005163"
                     /pseudo
     CDS             123..347
                     /locus_tag="ACHOUK_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(340..>590)
                     /locus_tag="ACHOUK_005164"
     CDS             complement(340..>590)
                     /locus_tag="ACHOUK_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9900386.1"
                     /translation="QFFRLLNRRYEKASIILTSNKGFADWGEMFGEHVLATAILDRLL
                     HHSTTLNIKGESYRFNIPDRLVVHLSLHARPELRAHNT"
BASE COUNT          145 a          149 c          148 g          148 t
ORIGIN      
        1 gcatataatc tttatcactc ttaaggttat tgtcccctca aaaaagtagc gctgatttcc
       61 tgtatgcgga atttgctgac taatcttacc cagcatcagc agacgttcca aaggccacga
      121 catacttcgc gaaacgcctg atggatgaac tgcgtgccca gggttaccat tttaaagtaa
      181 aaaccgtgac ggaaagcctg cgccgtcacg gactgagggc aaaagcttcc tggaatttca
      241 gtccggtctg ttaccgcgca cacagccagc ctgtgtcaga aaatctgctg gagcaggatt
      301 tttacgccag tggcccgaac cagaagtggg cgggagacat cacgtattgt gtgcccggag
      361 ttcagggcga gcatggaggc tcaaatgaac cacgagtctg tctggaatat tgaaccggta
      421 actctctcct ttgatattca gcgtggttga gtgatgtagc agccggtcca ggatcgctgt
      481 tgccagcaca tgctctccga acatttctcc ccagtctgcg aaccctttgt ttgacgtcag
      541 tatgatgctc gctttttcat atcgacggtt cagtagccgg aagaactgaa
//
LOCUS       DAZKIP010000407          578 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZKIP010000407 DAZKIP010000000
VERSION     DAZKIP010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.412"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            61..578
                     /locus_tag="ACHOUK_005165"
                     /pseudo
     CDS             61..578
                     /locus_tag="ACHOUK_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131642.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
BASE COUNT          163 a           99 c          136 g          180 t
ORIGIN      
        1 ggcagatgtt tgttaaagct atttaagtct ggagtttaaa ttaaaatagg gagttttata
       61 atgccgttaa attcggagat tagatcaagc tcatttttaa tggattgaat gtccttcgag
      121 ctcaagtagc atctagcggt cgaggggagt ttacattagg taatgagact gtcagcattg
      181 tatttaatga aaccgatggg cgttttctat ccagcggcag tagtggggga ttgcttactg
      241 agttattcct ttatgggttt aataacggcc ctgaagctct tcgcgatagg atgctcagta
      301 tgctttcgga ctcaggtgaa gcacaatcgc aagagagtat tcaggacaaa atatctcaat
      361 gtaagtttcc tgttagttca ggaaatttcc agtgcccgcc agagtctatt cagtgtccaa
      421 ttacactaga gagacccgaa gaaggagtgt ttgtcaaaaa ttcagatagt tcggcagtat
      481 gctgcttatt tgattttgat gcattttctc gtttagctag tgaaggctca tatcatccac
      541 tgacccgaga accaataacg gcatcaatga ttataagt
//
LOCUS       DAZKIP010000408          561 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZKIP010000408 DAZKIP010000000
VERSION     DAZKIP010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.413"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(397..>561)
                     /locus_tag="ACHOUK_005166"
     CDS             complement(397..>561)
                     /locus_tag="ACHOUK_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFP9900387.1"
                     /translation="FYKLCLLAVVILLLVMVMMDFTSRIMLVLTDGALVCGIVVLLWP
                     VIKRNSLHNA"
BASE COUNT          174 a          144 c          114 g          129 t
ORIGIN      
        1 gccgggatag tgtattaaat cagatatgga gtgggctgta gtccggaagc ctgaatgaca
       61 cacggggact acagcccaag aaatgaaaaa aggccacgca gttgcgcagc ctgataaacc
      121 ctggttaaaa tccacacgat aacaacacaa caatatcagt atctcatgct attgcccgaa
      181 cccattcggg cattttttac ccataaaaaa tgcccctccg gagaggggca tgtttgcatg
      241 cacattcttt ttcttgcatg gtgccgggtg cctcccggtg aattcagtat cagcacctga
      301 atccgcgatt atcacatata cctggttgct gattgcccct ccgcacaggg ggattcacca
      361 tgcagtagta tttttaataa acagtaaaca aaaaaatcaa gcattatgca ggctgtttct
      421 ttttatcacc ggccacagca ataccacaat gccgcagacc agagcgccat cagtcagtac
      481 caacattatc ctgctggtga aatccatcat caccatcact aaaagcagga tcacaacagc
      541 aagcagacac agtttataaa a
//
LOCUS       DAZKIP010000409          555 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZKIP010000409 DAZKIP010000000
VERSION     DAZKIP010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.414"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..220
                     /gene="escG"
                     /locus_tag="ACHOUK_005167"
     CDS             <1..220
                     /gene="escG"
                     /locus_tag="ACHOUK_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE needle protein
                     cochaperone EscG"
                     /protein_id="HFP9900388.1"
                     /translation="HKLPKYAEAILNVFPQIIPDKKDIAHLEFIILFGLNRKNDAVKA
                     LEDCMDDETSQLLYSLVHENGSGWVRGF"
     gene            305..>555
                     /locus_tag="ACHOUK_005168"
     CDS             305..>555
                     /locus_tag="ACHOUK_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspF"
                     /protein_id="HFP9900389.1"
                     /translation="MLNGISNAASTLGRQLVGIASRVSSAGGTGFSVAPQAVRLTPVK
                     VHSPFSPGSSNVNARTIFNVSSQVTSFTPSRPAPPPPTSG"
BASE COUNT          154 a          102 c          128 g          171 t
ORIGIN      
        1 ccataagctt cccaaatatg cggaagctat cctgaatgta ttcccacaaa ttatacctga
       61 taaaaaagat atcgcacatt tagaatttat tatcttattt ggattaaata gaaaaaatga
      121 tgcggtaaag gctttggagg actgtatgga tgatgagacg agtcagttat tgtacagttt
      181 ggtccatgag aatggtagtg gctgggtacg aggattttaa tttatattga tttaaaaatc
      241 attttattat tcgttttgat atatatgaga gttagccaag cttagatata aagaggcata
      301 aattatgctt aatggaatta gtaacgctgc ttctacacta gggcggcagc ttgtaggtat
      361 cgcaagtcga gtgagctctg cggggggaac tggattttct gtagcccctc aggccgtgcg
      421 tcttactccg gtgaaagttc attccccttt ttctccaggc tcgtcgaatg ttaatgcgag
      481 aacgattttt aatgtgagca gccaggtgac ttcatttact ccctctcgtc cggcaccgcc
      541 gccaccgaca agtgg
//
LOCUS       DAZKIP010000410          555 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZKIP010000410 DAZKIP010000000
VERSION     DAZKIP010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.415"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(254..328)
                     /locus_tag="ACHOUK_005169"
                     /pseudo
     CDS             complement(254..328)
                     /locus_tag="ACHOUK_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312679.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          183 a           95 c          113 g          164 t
ORIGIN      
        1 tgatgtagtt caaaatatca ggacttggct aaaagaaaat caaaatatgg tgaaagctat
       61 taagaaaatt gatactgaat aacatggcga ataaataaac ggatcaagtc gttcctatga
      121 caatgaggtt atacgaaaag acgcttactc atcagaaatc tggtatgctt taattaaacc
      181 agtgtcgctg atgtgtaagc gttgacgttg ggagacgatt aaatggttat accactccta
      241 cgtcttttcc atacggcgta taagagtgca acatgttgag ttttccgagt gaatggtgca
      301 ttttactaac cgagccattt gaatcaacca acgcaataaa aaagactgga ataataagcc
      361 tgtatttatt atttatcgca atcgcacttt acggttttat ccaactgaaa agagactgct
      421 tccagcggtt gtggattatc cctgggcgat agtcatagtc tggtgtcagg attcacaaat
      481 gacctgtttc cttacatgga tataaacaca acatagagtg cggtagttaa tctgaataat
      541 ctaatttcgg ctatt
//
LOCUS       DAZKIP010000411          550 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZKIP010000411 DAZKIP010000000
VERSION     DAZKIP010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.416"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..>550)
                     /locus_tag="ACHOUK_005170"
     CDS             complement(<1..>550)
                     /locus_tag="ACHOUK_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9900390.1"
                     /translation="SFRQKDADLQSQYQTGDISEDFYRQALAQNAQYLSERLKDQAVF
                     YAESDVQRADWQKGLQEGFSNWVDNASDYASQAAQLATEGISGMVNNITEMLNGNKVE
                     WRSWASSVLQEISKVLMNAAIVNGIKTAANGMSGAGGFLGSIGDWLGGAVANAKGGVY
                     TSANLSAYSNSIVDTPTYFAFA"
BASE COUNT          109 a          175 c          124 g          142 t
ORIGIN      
        1 tttgcaaacg caaagtacgt gggcgtgtcc acaatgctgt tgctgtacgc gctcaggttt
       61 gccgaggtat acacgccgcc ttttgcattg gccaccgctc cgcccagcca gtcaccaatg
      121 ctgccgagaa atcctcccgc accggacata ccgtttgccg ccgtcttaat tccgttgaca
      181 atcgcggcat tcataagaac ttttgatatt tcctgcagca ctgatgaggc ccagctgcgc
      241 cattccactt tatttccgtt cagcatctcc gtgatgttat tcaccatccc tgagataccc
      301 tccgtcgcca gctgtgctgc ctgtgaggcg taatcggacg cattatccac ccagttactg
      361 aatccctcct gcagcccttt ctgccagtcc gcacgctgca catccgattc ggcataaaag
      421 actgcctggt ccttaaggcg ttcgctcaga tactgcgcgt tctgtgccag agcctgtctg
      481 taaaaatcct cactgatatc cccggtctga tactgagact gaaggtccgc atccttctgg
      541 cggaagctgt
//
LOCUS       DAZKIP010000412          531 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZKIP010000412 DAZKIP010000000
VERSION     DAZKIP010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.417"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..310)
                     /locus_tag="ACHOUK_005171"
     CDS             complement(<1..310)
                     /locus_tag="ACHOUK_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFP9900391.1"
                     /translation="MSEINYQALREKAEKATKGSYIVGHTSVNQHGNLTGVFVCQKWK
                     GEPGGVIAECHVNCLVETDVQAYANAEFIAAFNPNVALALLDERERNQQYIKRRDQEN
                     E"
     gene            complement(307..528)
                     /locus_tag="ACHOUK_005172"
     CDS             complement(307..528)
                     /locus_tag="ACHOUK_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFP9900392.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNYQTVSATHCCECGDPIDE
                     RRRLAVPGCRTCASCQEDLELISKQRGSK"
BASE COUNT          120 a          141 c          103 g          167 t
ORIGIN      
        1 cctcgttctc ctggtcgcgg cgtttgatgt attgctggtt tctttcccgt tcatccagaa
       61 gcgccagcgc aacatttgga ttaaaggcag caataaattc agcgtttgca taagcctgaa
      121 catctgtttc aaccaggcag ttaacatgac attccgcaat cacaccaccg ggttctcctt
      181 tccatttttg gcaaacaaaa actcctgtta aattgccgtg ctggttaaca gatgtatgcc
      241 ctacgatgta gcttccttta gttgctttct ctgccttttc acgcagtgcc tgataattaa
      301 tttcgctcac ttcgaacctc tctgtttact gataagctcc agatcctcct ggcaacttgc
      361 acaagtccga caacccggaa ctgccaggcg tcttcgttca tctatcggat cgccacactc
      421 acaacaatga gttgcggata cagtctggta gttcaggcga cgcattttta ttgctgtatt
      481 gcgctgtaat tcttcaattt ctgatgctga atcaatgatg tctgccatct t
//
LOCUS       DAZKIP010000413          531 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZKIP010000413 DAZKIP010000000
VERSION     DAZKIP010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.418"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..310)
                     /locus_tag="ACHOUK_005173"
     CDS             complement(<1..310)
                     /locus_tag="ACHOUK_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFP9900393.1"
                     /translation="MSEINYQALREKAEKATKGSYIVGHTSVNQHGNLTGVFVCQKWK
                     GEPGGVIAECHVNCLIESDAQAYANAEFIAEANPATVLELLDERERNQQYIKRRDQEN
                     E"
     gene            complement(307..528)
                     /locus_tag="ACHOUK_005174"
     CDS             complement(307..528)
                     /locus_tag="ACHOUK_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFP9900394.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNYQTVSATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEDLELISKQRGSK"
BASE COUNT          112 a          142 c          105 g          172 t
ORIGIN      
        1 cctcgttctc ctggtcgcgg cgtttgatgt attgctggtt tctttcccgt tcatccagca
       61 gttccagcac ggtagccggg ttagcctctg ctatgaattc agcgtttgca taagcctgag
      121 catctgattc aatcaggcag ttaacatgac attccgcaat cacgccaccg ggttctcctt
      181 tccatttttg gcaaacaaaa actcctgtta aattgccgtg ctggttaaca gatgtatgcc
      241 ctacgatgta gcttccttta gttgcttttt ctgccttttc acgcagtgcc tgataattaa
      301 tttcgctcac ttcgaacctc tctgtttact gataagctcc agatcctcct ggcaacttgc
      361 acaagtccga caaccctgaa ctgccaggcg tcttcgttca tctatcggat cgccacactc
      421 acaacaatga gttgcggata cagtctggta gttcaggcga cgcattttta ttgctgtatt
      481 gcgctgtaat tcttcaattt ctgatgctga atcaatgata tctgccatct t
//
LOCUS       DAZKIP010000414          526 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZKIP010000414 DAZKIP010000000
VERSION     DAZKIP010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.419"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>526
                     /gene="rfbE"
                     /locus_tag="ACHOUK_005175"
                     /gene_synonym="perA"
     CDS             <1..>526
                     /gene="rfbE"
                     /locus_tag="ACHOUK_005175"
                     /gene_synonym="perA"
                     /EC_number="2.6.1.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-perosamine synthase RfbE/PerA"
                     /protein_id="HFP9900395.1"
                     /translation="DISTFSFFGNKTITTGEGGMVVTNDKTLYDRCLHFKGQGLAVHR
                     QYWHDVIGYNYRMTNICAAIGLAQLEQADDFISRKREIADIYKKNINSLVQVHKESKD
                     VFHTYWMVSILTRTAEEREELRNHLADKLIETRPVFYPVHTMPMYSEKYQKHPIAEDL
                     GWRGINLPSFPSLS"
BASE COUNT          179 a           92 c          104 g          151 t
ORIGIN      
        1 gagatatttc tacttttagc ttttttggaa ataaaactat tactacaggt gaaggtggaa
       61 tggttgtcac gaatgacaaa acactttatg accgttgttt acattttaaa ggccaaggat
      121 tagctgtaca taggcaatat tggcatgacg ttataggcta caattatagg atgacaaata
      181 tctgcgctgc tataggatta gcccagttag aacaagctga tgattttata tcacgaaaac
      241 gtgaaattgc tgatatttat aaaaaaaata tcaacagtct tgtacaagtc cacaaggaaa
      301 gtaaagatgt ttttcacact tattggatgg tctcaattct aactaggacc gcagaggaaa
      361 gagaggaatt aaggaatcac cttgcagata aactcatcga aacaaggcca gttttttacc
      421 ctgtccacac gatgccaatg tactcggaaa aatatcaaaa gcaccctata gctgaggatc
      481 ttggttggcg tggaattaat ttacctagtt tccccagcct atcgaa
//
LOCUS       DAZKIP010000415          525 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZKIP010000415 DAZKIP010000000
VERSION     DAZKIP010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.420"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..>525)
                     /locus_tag="ACHOUK_005176"
     CDS             complement(<1..>525)
                     /locus_tag="ACHOUK_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900396.1"
                     /translation="LHFYDYSRRYAINENIAKDAVTNNIALMSTFYDSDNTSGEVLNN
                     DFTEESFETALDVEHTIVLGFADDNFETKPVHYQDLLTRFSAFQDTVFNLFPEMHSSH
                     YNDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMVREDASNLINLKTLLNDGCF
                     IKYSHFNDVALIKQ"
BASE COUNT          175 a          101 c           77 g          172 t
ORIGIN      
        1 tttgctttat taatgccacg tcattaaaat gactatattt aataaagcaa ccatcattga
       61 gtagtgtttt aagattgatt aaattacttg catcttcacg taccatcgtg gcaactggaa
      121 caggcagaga catgaaaact tcttcattca ccatcaagtg aatcatgcat tgcgttccct
      181 ttgtcatatc cacacttacg ctacaaatat cgttgtaatg cgaagagtgc atttcaggaa
      241 acagattaaa cacggtatct tgaaaggcac taaatcgtgt taataaatcc tgataatgaa
      301 caggttttgt ttcaaaatta tcatcagcaa atccaagcac tattgtatgc tctacatcta
      361 acgcagtttc aaatgactct tctgtaaaat cattatttaa aacctcacct gacgtattat
      421 cactgtcata aaaagtgctc atcaatgcga tattgtttgt tacagcgtct tttgcaatat
      481 tttcattaat tgcatatcgc ctgctataat cataaaaatg aagaa
//
LOCUS       DAZKIP010000416          519 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZKIP010000416 DAZKIP010000000
VERSION     DAZKIP010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.421"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            278..>519
                     /locus_tag="ACHOUK_005177"
     CDS             278..>519
                     /locus_tag="ACHOUK_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900397.1"
                     /translation="MATQTEVARHLSLTDRQLRRLQKLPGAPISNKRGQLDLDAWRDF
                     YISYLRRSKNDVPDGDSEDDYEEKLLIARWELTAEQA"
BASE COUNT          141 a           98 c          146 g          134 t
ORIGIN      
        1 gatggcgtag atgcacgttg tgacatgtag atgataatta ttatcatttt gcgggtcctt
       61 tccggcgatc cgacaggtta cggggcggcg acctcgcggg ttttcgctat ttatgaaaat
      121 tttccgggat ccatgtccgg tttctcttca agttaactat atgaaaaata taaaaacagg
      181 tcttctgtga accggacatg aacaaaaaac agacatgtaa accggacatg accggttttg
      241 ttgtgattgt gaggtgagag tttttgcgag gtgaggagtg gctacgcaga ctgaagttgc
      301 caggcattta agtctgaccg atcgccagct tcgcagattg cagaaattgc cgggtgcccc
      361 gatatcgaat aagcgagggc aactggatct ggatgcctgg cgcgattttt acatatcgta
      421 tctgaggaga agtaaaaacg atgtgcctga tggcgatagc gaagacgact atgaggagaa
      481 attgcttatt gccagatggg aactgacagc agaacaggc
//
LOCUS       DAZKIP010000417          511 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZKIP010000417 DAZKIP010000000
VERSION     DAZKIP010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.422"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            26..451
                     /gene="yqeK"
                     /locus_tag="ACHOUK_005178"
     CDS             26..451
                     /gene="yqeK"
                     /locus_tag="ACHOUK_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeK"
                     /protein_id="HFP9900398.1"
                     /translation="MDIEFSQIHEMVYMHDIVNSDSKKKPRIPLKKFLNAEKVLTQTT
                     SWALNSRFVNVNSVNKVNVKSKVKNSYISRSVNDEFSLTDDEINSFKETLVLSSIDSL
                     SKMVLNNPLSVLFTSTVRRNNNRAKMNVEFDSWICTRCC"
BASE COUNT          179 a           71 c           95 g          166 t
ORIGIN      
        1 tcacggtaaa aatgtgaggt atataatgga cattgagttt tcgcagattc atgaaatggt
       61 ctatatgcat gatattgtaa attcggactc gaaaaagaaa ccaaggattc cgttaaaaaa
      121 attccttaat gctgagaagg ttctcacgca aacaacatca tgggcattaa attcaagatt
      181 tgtgaatgtt aattctgtca ataaagtcaa cgtaaagagt aaagttaaaa acagttatat
      241 ctcacgatct gtcaatgatg agtttagttt aacagatgat gaaatcaatt cttttaaaga
      301 aacgttagtc ctttcctcta ttgattcact gagtaaaatg gttcttaata atcctctttc
      361 tgtattgttt acatcgactg tacgaaggaa taataatcga gctaagatga atgtcgagtt
      421 cgactcatgg atttgcacca gatgttgttg atacatgata ttttacattg taaggagatg
      481 tctaaaatga aagatgttga tcaaaacttt g
//
LOCUS       DAZKIP010000418          510 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZKIP010000418 DAZKIP010000000
VERSION     DAZKIP010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.423"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>510
                     /locus_tag="ACHOUK_005179"
     CDS             <1..>510
                     /locus_tag="ACHOUK_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900399.1"
                     /translation="QSGLWGKISQWSGINNQGREDYRKRDFAPDSPEIKFSDRREALE
                     LMTQIESTVTSLHREAEAQFRPELEKIVSGIETGFRGTALYATENIAGRINARLEDEG
                     FTVKISFPAVSQLQTRLAVKINLSALMEERTETVTRRRRQSGVWGTVCRWFGTSDLGW
                     ENYDEDVSR"
BASE COUNT          134 a          124 c          153 g           99 t
ORIGIN      
        1 ctcagtccgg attatggggg aaaatttctc aatggtccgg tatcaacaac cagggccggg
       61 aggattacag gaaacgagat tttgccccgg acagccccga aataaaattc agtgatcgca
      121 gggaagccct tgaactgatg acgcaaatcg aatcgaccgt gaccagcctg caccgtgagg
      181 ctgaagcaca gttccggcct gagctggaga aaatcgtcag cgggattgaa acaggttttc
      241 gtggcacggc cctgtacgcc acagaaaaca ttgccggtcg catcaatgcc cgcctggagg
      301 atgagggctt taccgtaaaa atcagttttc cggcagtcag ccagttacag acccggctcg
      361 cggtaaaaat aaatctgagt gcgcttatgg aggaaagaac ggagaccgtc acccgtcgcc
      421 gtcggcagag tggcgtatgg ggaaccgttt gtcgatggtt tggcaccagt gacctgggct
      481 gggaaaacta tgacgaggat gtgagtcgca
//
LOCUS       DAZKIP010000419          504 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZKIP010000419 DAZKIP010000000
VERSION     DAZKIP010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.424"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..302
                     /locus_tag="ACHOUK_005180"
     CDS             <1..302
                     /locus_tag="ACHOUK_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFP9900400.1"
                     /translation="LLDILRHKALTQMAQESGGSATVRLNTLDWLGGQGREQADNEWH
                     DAINWLGDWCSEEQHPVIWSTTQAAEHLPVRMPRLCSAERLSESMVDEIFQKGAA"
     gene            299..>504
                     /locus_tag="ACHOUK_005181"
     CDS             299..>504
                     /locus_tag="ACHOUK_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFP9900401.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGASIG
                     AFNGAIIAASTDLSEAAVRLEALW"
BASE COUNT          114 a          116 c          170 g          104 t
ORIGIN      
        1 agctactgga tattctcaga cataaagcat taacgcagat ggcacaggag agtggcggta
       61 gtgcaaccgt gcgtctgaac actctggact ggcttggtgg tcagggccgg gagcaggcag
      121 ataacgagtg gcatgacgcg attaactggc tgggagactg gtgcagtgaa gagcagcatc
      181 cggtgatctg gtcaaccacg caggctgcag aacacttacc ggttcgcatg cctcgcctct
      241 gctctgcaga acgcctctct gaaagtatgg tcgatgaaat atttcagaag ggggctgcat
      301 gagtacagaa atgaaaacgg ggctggtgct gtccggtggc ggggcggtgg gcgcttatca
      361 ggcgggagtg gttaaggcac tggcagagtg tggtacacag atcagcatgg tttcaggggc
      421 cagcattggc gcattcaatg gtgccattat cgcggcctct accgatctgt cagaagctgc
      481 cgtacgcctg gaggcgctct ggga
//
LOCUS       DAZKIP010000420          503 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZKIP010000420 DAZKIP010000000
VERSION     DAZKIP010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.425"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..89
                     /locus_tag="ACHOUK_005182"
     CDS             <1..89
                     /locus_tag="ACHOUK_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFP9900402.1"
                     /translation="SFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(114..>503)
                     /locus_tag="ACHOUK_005183"
     CDS             complement(114..>503)
                     /locus_tag="ACHOUK_005183"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900403.1"
                     /translation="KMFKSSIRNIFYPSKLERLSRFQFIIYTLTSWSILITLNDINLA
                     LSFILVINNTYVNINIGAKRIRDTGCKAIYFVLISLGLTLFSIISAAIEGVRCESLSV
                     QLNVAWILFTTIMLSAIPSAKMSLSAH"
BASE COUNT          158 a           90 c          100 g          155 t
ORIGIN      
        1 ggagtttcgg ctacccaggc gcgattatct ttatgatcct cgcgggcctg gcaccgtatc
       61 tgtactttaa gcgcaagaac tggctgtaac tatacgatgt cgctgtgagt aagttagtgt
      121 gctgacagcg acattttagc agagggaatc gcagataaca taattgttgt gaataatatc
      181 catgccacgt tgagttggac gcttaatgac tcacatctca ccccttcaat tgctgcagag
      241 ataatagaaa aaagtgtcaa acccaatgaa atcaatacaa agtagattgc cttacatcca
      301 gtatctctta ttctttttgc tccaatattg atatttacat aggtattatt aataaccaga
      361 ataaaactca aagccaggtt gatatcattc aaagttatta atattgacca tgatgttaat
      421 gtatagatta tgaactgaaa gcgtgataaa cgctcaagct tggatgggta gaatatattt
      481 ctgatggatg atttgaacat ttt
//
LOCUS       DAZKIP010000421          497 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZKIP010000421 DAZKIP010000000
VERSION     DAZKIP010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.426"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..197)
                     /locus_tag="ACHOUK_005184"
     CDS             complement(<1..197)
                     /locus_tag="ACHOUK_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFP9900404.1"
                     /translation="MAVLRTLPGRIKTLNTRRVNVLKGEQRRVSGSARVSLKRRIWLR
                     DAGQCCLCGRVVDLCDSELDHR"
     gene            239..466
                     /locus_tag="ACHOUK_005185"
     CDS             239..466
                     /locus_tag="ACHOUK_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFP9900405.1"
                     /translation="MSSKNRTRRTTTRNIRFPNQMIEQINIALDQKGSENFSAWVIES
                     CRRELAADIKYARQLTIKKNDTQYALRWLFI"
BASE COUNT          153 a          129 c          104 g          111 t
ORIGIN      
        1 cggtgatcga gttcactgtc acaaaggtca accacacgcc cacagagaca gcactgtccg
       61 gcatccctga gccagatacg acgcttgagg gaaacccggg cactgccact gacacgacgc
      121 tgttcaccct tcaggacatt cacccgccgg gtgttcagag ttttgattct gcccggtaac
      181 gtacgaagca cagccatgta aaatcctcgc catataactt gtcaccagag gaaagaaaat
      241 gtcatcgaaa aaccggaccc gcagaacaac aacccgcaac atccgatttc caaaccagat
      301 gattgaacaa attaacatcg ctcttgacca gaaaggttca gaaaattttt ctgcgtgggt
      361 cattgaatct tgccgccggg agctggcagc agacataaaa tatgcccgtc agttgactat
      421 aaaaaagaat gatacacagt atgctctgcg atggctgttc atataactat ttctttatat
      481 tgctgaattt ataaaaa
//
LOCUS       DAZKIP010000422          496 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZKIP010000422 DAZKIP010000000
VERSION     DAZKIP010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.427"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
BASE COUNT          141 a           97 c           94 g          164 t
ORIGIN      
        1 gtcctgaaaa aatacttttt attgaaaatg gcatttttat cctcacatta tgtctttctc
       61 ttatagtgca atttaatgta tttagaaaca ttaaactatg aatctaaaaa ggctacgcag
      121 agtactgtta cactttaaca aatcatattt accactctgg aaatgggatg gctggttcat
      181 aaaataatga gtctttttca acggttgtta cattggtgta ctgatgatta tgccttgtcg
      241 ctaagagcta ccataaccgt acagcgtgat tcatccatgg cgaataaatc gactgataaa
      301 tccccctgag tttcagctct tgctgttgga ccttgcccgt ttcgctatgg aacgcgtacc
      361 agctagagaa tattattcgt tctcctccaa tgttacgcaa gttttgtaaa cggtaaccaa
      421 tcgtgattag tcctgcatgt aacggacatc aagctgtctg gttgattttt attgccagta
      481 gttcgagaga gcagac
//
LOCUS       DAZKIP010000423          488 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZKIP010000423 DAZKIP010000000
VERSION     DAZKIP010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.428"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            71..488
                     /locus_tag="ACHOUK_005186"
                     /pseudo
     CDS             71..488
                     /locus_tag="ACHOUK_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
BASE COUNT          161 a           73 c           97 g          157 t
ORIGIN      
        1 tttggtcatg aaatttccgt cttgttacac tactctaaac tagagcttat ggaattagcg
       61 ggtggtattc atggaaaata atgataaatt cttatcacaa gacttattgg aatcttatgc
      121 cattcgtttg ttgagcggac catagaatgg atgcgagtat gaaatactta atgggcgtct
      181 acttgttatc attggtaatg atgtttcgtt aggaagaagt gatgcttttt ctgagttacc
      241 agaaaataca atagtcgttc cttatggcga gcttacaggt agttttgaga ttataatcac
      301 taccgaccct gatctggttg taacaatcag agaattaaca gcacaagaac ctgaagatag
      361 aacgttaaca ttgaatcagc aaatagaagt tttaggtctc aaatttgctg taaaagaaaa
      421 aatgaagttt ggcagtattc attgccaggc attattgaaa atagcattat ttctacaaaa
      481 caacattt
//
LOCUS       DAZKIP010000424          486 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZKIP010000424 DAZKIP010000000
VERSION     DAZKIP010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.429"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>486
                     /locus_tag="ACHOUK_005187"
     CDS             <1..>486
                     /locus_tag="ACHOUK_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HFP9900406.1"
                     /translation="EILRYITVQRISEVIQRLIQERISVRNMRLVMEALALWSPREKD
                     IITLVEHVRGALGRYICHKFSYSGEIKAIVISPEIEDRIRDGVRPTAGGTFLNLDASE
                     AEMILDNFKLALSGINIPIKDIILLGSVDIRRFIKKLIESSYRDLEVLSYGELTENVP
                     VN"
BASE COUNT          144 a           87 c          109 g          146 t
ORIGIN      
        1 gaaatattaa gatatataac cgtgcagcgc atttcagagg taattcagcg tctgatccag
       61 gagcgaattt cagtgcgcaa tatgcggtta gttatggagg ccttagcatt atggagccca
      121 cgagagaaag atataatcac actcgtcgag catgtacgag gtgcattagg tcgctacatt
      181 tgccataagt tttcatattc tggagaaatt aaagcaattg tgatttcgcc tgaaatagag
      241 gacagaatca gagatggtgt caggcctacc gctggcggta cttttcttaa tctcgatgca
      301 tccgaagctg agatgattct ggataatttt aaactggcac tttctggaat caatattcct
      361 attaaagaca ttattttact gggatctgtg gatattcgtc gttttatcaa aaaacttatt
      421 gaatctagtt atcgagacct cgaagtactt tcgtacgggg aactaacgga aaatgttccc
      481 gttaat
//
LOCUS       DAZKIP010000425          480 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZKIP010000425 DAZKIP010000000
VERSION     DAZKIP010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.430"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>480
                     /locus_tag="ACHOUK_005188"
     CDS             <1..>480
                     /locus_tag="ACHOUK_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HFP9900407.1"
                     /translation="SDKIALSINDKKYNVNSKDIENILDGKGDLFKKRTLWEFVRDLF
                     PGSHIKEVKGLIYEFVTKVDNKAEVFDKIKSLAKKEQQWRFSTKTDFTTNENNEVIVS
                     RSFNLYTGATSNDNEKKQVSSERLTLDNYLDDLHFDNSPLVRLTFDNYSVKLATLIKN
                     "
BASE COUNT          173 a           65 c           88 g          154 t
ORIGIN      
        1 tctgataaaa ttgctttaag tattaatgat aagaaatata atgtaaactc taaggatatt
       61 gaaaatattc ttgatggcaa aggggattta tttaaaaaac gtacactgtg ggagtttgtt
      121 cgcgatcttt ttcctggctc tcatattaaa gaagtcaaag gcttaattta cgaatttgtc
      181 acgaaagttg ataataaagc cgaggtgttc gataagatta aatctctggc aaaaaaagaa
      241 caacaatggc gatttagtac taagacagac tttaccacaa atgaaaataa tgaagttatt
      301 gtgagtagat cctttaatct ctacacgggg gctacttcga atgataacga aaaaaaacag
      361 gtttcttcag agagattaac gcttgataat tatctcgatg atttacattt tgataattct
      421 cccttggtga gattaacgtt tgataattat tctgtaaaac ttgcgacgct gattaaaaat
//
LOCUS       DAZKIP010000426          462 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZKIP010000426 DAZKIP010000000
VERSION     DAZKIP010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.431"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..228
                     /locus_tag="ACHOUK_005189"
     CDS             <1..228
                     /locus_tag="ACHOUK_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9900408.1"
                     /translation="PLTRAADGSLGVRAVGSMNGSAGLVYSPVYHIAIQNDGANGQIG
                     PEAAGSLVQLIDQRVQAVMLSMRRDGGMLSG"
     gene            221..>462
                     /locus_tag="ACHOUK_005190"
     CDS             221..>462
                     /locus_tag="ACHOUK_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9900409.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVVRVRFGDGYEQRRPTGLNPQL
                     KTFQAVFRVTDESTRRWLDEFLSWHGGYRAFLWRPP"
BASE COUNT           85 a          112 c          169 g           96 t
ORIGIN      
        1 cccctgaccc gggcggcgga tggctcgctg ggcgtacgcg ccgtgggcag tatgaacggc
       61 agtgctggtc tggtgtattc cccggtctac cacatcgcca ttcagaatga cggggctaac
      121 ggacagatag ggccggaggc ggcaggcagt cttgtgcagc tgattgacca gcgggtgcag
      181 gcggtgatgc tgtccatgcg acgtgacgga ggaatgctga gtggctgaga taaaaacgct
      241 gcatctggtc ccgcgtgaag ggatgcaggt gagtgagaag ccgtcggtgg tgagggtgcg
      301 gtttggtgac ggttatgaac agcgccgccc cacagggctg aatcctcaac tgaagacgtt
      361 tcaggcggtg ttccgggtga cggatgagtc aacccggcgc tggctggatg aatttttatc
      421 ctggcatggt ggttaccgtg cctttttgtg gcgaccgccg aa
//
LOCUS       DAZKIP010000427          457 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZKIP010000427 DAZKIP010000000
VERSION     DAZKIP010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.432"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..348
                     /locus_tag="ACHOUK_005191"
     CDS             <1..348
                     /locus_tag="ACHOUK_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900410.1"
                     /translation="SRADLLADRKKRFRKNPSLGMGDWRFFISEPGIISIEDLPPGWG
                     LLHVVNGRVRKVHGWPKGNCCWGNPDDKPFTGNKQVECDYMLSALRRMELRGHLNEIY
                     DGVIVNKKEGNAA"
     gene            345..>457
                     /locus_tag="ACHOUK_005192"
     CDS             345..>457
                     /locus_tag="ACHOUK_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valyl-tRNA synthetase"
                     /protein_id="HFP9900411.1"
                     /translation="MTTITDKELIKEIKERIGSLDVRDNIERRAYEIALASL"
BASE COUNT          136 a           77 c          123 g          121 t
ORIGIN      
        1 tctcgtgctg acttgttggc agatagaaaa aagcgttttc gtaaaaatcc gtctcttgga
       61 atgggcgact ggcgattctt tattagtgag ccgggaatta tttcaattga ggatttacca
      121 cctggctggg gattacttca cgttgttaac ggaagagtac ggaaagtaca tgggtggccc
      181 aagggtaatt gctgttgggg taatcctgac gataagccat ttactggaaa taagcaggtt
      241 gaatgcgatt acatgttgtc tgcattaagg cgcatggagt tgagagggca ccttaatgaa
      301 atatatgacg gtgtaattgt taataagaaa gaaggaaacg cggcatgacc actattaccg
      361 acaaagaact gattaaagaa atcaaagagc gcataggcag cttggacgtt cgagacaata
      421 ttgagcgccg tgcttatgaa attgcactgg catcgct
//
LOCUS       DAZKIP010000428          454 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZKIP010000428 DAZKIP010000000
VERSION     DAZKIP010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.433"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>454
                     /gene="espR3"
                     /locus_tag="ACHOUK_005193"
     CDS             <1..>454
                     /gene="espR3"
                     /locus_tag="ACHOUK_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector leucine-rich repeat protein EspR3"
                     /protein_id="HFP9900412.1"
                     /translation="KTGSITIEKSQGEGTLPIRHKLEFISTNIAELLDKLTKITDARL
                     CKGFSDWASSVKEGASNDLKENVDRALVRMFKCVKLHSNELNLSSLSLGSVPPLPEWI
                     EMLSLVYNELDSIQVPESCKELELDFNNLTEFPQVPDGITLISVNNNLI"
BASE COUNT          145 a           81 c           99 g          129 t
ORIGIN      
        1 aagacgggat ctatcacgat agaaaaaagt cagggggagg ggactttacc cattcggcat
       61 aaattagagt ttatatcgac caatattgca gagttgctgg acaagttaac caaaattaca
      121 gatgccaggc tttgcaaagg tttcagtgac tgggctagtt cagtcaaaga aggcgcatcg
      181 aatgacttga aagaaaatgt ggatagagca ttggtgagaa tgtttaaatg tgttaagctt
      241 cacagtaatg aacttaactt atcaagcctt tctttgggtt ctgtgccgcc tcttcctgag
      301 tggattgaaa tgcttagcct tgtttataat gaacttgatt caatacaggt gcccgaatcg
      361 tgcaaagaat tagaactcga tttcaataac cttacagaat ttccacaagt acctgatgga
      421 attaccctga tctccgtaaa taataacctg atat
//
LOCUS       DAZKIP010000429          450 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZKIP010000429 DAZKIP010000000
VERSION     DAZKIP010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.434"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            158..373
                     /locus_tag="ACHOUK_005194"
     CDS             158..373
                     /locus_tag="ACHOUK_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP9900413.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            378..>450
                     /locus_tag="ACHOUK_005195"
     CDS             378..>450
                     /locus_tag="ACHOUK_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
                     /protein_id="HFP9900414.1"
                     /translation="MKKKYELVVKGINNYPDKITVTVA"
BASE COUNT          123 a           83 c          135 g          109 t
ORIGIN      
        1 catcataaaa agtgagccag tcaaatggaa ggtatcgtta aactcaccgg tagtgtcagt
       61 gggtcgtctg agacgcctgc atgagttatc agagccatca gtagttaact ggtggctttt
      121 ttattgttgt cagcttccgg ataacgggag acggggtatg taccagatgg aaaaaatcac
      181 aacgggtgtg tcatacacca cgtcagcggt ggggacggga tactggttac tgcagctgct
      241 ggacaaagtc tctccgtccc agtgggtggc gataggcgtg ctggggagtc tgctgtttgg
      301 gctgctgaca tatctgacga acctgtattt caagattaaa gaagaccggc gtaaggcggc
      361 gcggggagag taaagtgatg aagaaaaaat acgaactggt tgttaaaggg ataaataatt
      421 acccggataa gattactgtt actgtggcac
//
LOCUS       DAZKIP010000430          442 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZKIP010000430 DAZKIP010000000
VERSION     DAZKIP010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.435"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..>442)
                     /locus_tag="ACHOUK_005196"
     CDS             complement(<1..>442)
                     /locus_tag="ACHOUK_005196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP9900415.1"
                     /translation="STPEGEWYIPCVNISAEPEAYFRIAPEDWLRAEMQGEIVALVHS
                     HPGGLPWLSEADRRLQIKSALPWWLVCRGDIHKFRCVPHLTGRRFEHGVTDCYTLFRD
                     AYHLAGTDMPDFEREDDWWRNGQNLYLDNMEATGFYRISLPSAQP"
BASE COUNT           99 a          150 c          108 g           85 t
ORIGIN      
        1 ggctgtgcgg aaggcaggga aatcctgtaa aaaccagtcg cctccatatt gtccaggtac
       61 aggttctgac cattgcgcca ccagtcatcc tcacgctcaa aatccggcat atcagtcccc
      121 gccagatgat aagcatcccg gaacagcgtg taacagtccg tcaccccgtg ctcaaagcgc
      181 cgtcctgtca gatgtggcac acagcggaat ttgtgaatgt caccccggca gaccagccac
      241 cagggcagtg cgctttttat ctgcagccgc cggtcggcct cgctcagcca gggcagccca
      301 ccgggatgac tgtggaccag tgccacaatc tccccctgca tctctgcccg cagccagtct
      361 tccggtgcga tacgaaaata cgcctccggc tctgcagaaa tattcacaca agggatatac
      421 cactccccct ccggcgtgct ta
//
LOCUS       DAZKIP010000431          441 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZKIP010000431 DAZKIP010000000
VERSION     DAZKIP010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.436"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..304
                     /locus_tag="ACHOUK_005197"
     CDS             <1..304
                     /locus_tag="ACHOUK_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HFP9900416.1"
                     /translation="SSGGGFMEILLVKRQNMKIKIYQEKGHQLPHIHIDYGKQAHAAS
                     YAIQSGDRIEGDLPKKYDSDVSSWLGRNRDKILEIWNALQAGAPYEPLIAELTGGV"
BASE COUNT          153 a           77 c           98 g          113 t
ORIGIN      
        1 ctctagtggc ggcggattca tggaaattct cttggtgaag agacagaaca tgaagataaa
       61 aatatatcaa gaaaaaggac atcaacttcc ccatattcat attgactatg gaaaacaagc
      121 gcatgctgcc agttatgcca tacaatcagg agaccgcata gagggagatt tacctaagaa
      181 atatgatagt gatgtttcga gttggttggg gagaaatcgt gataaaattt tagaaatctg
      241 gaatgcacta caagctggcg caccttatga accactaatc gcagaattga ctggcggagt
      301 gtgactaact aaataatttt cccctaaaaa aattcgatgt aggaaaatat atataagtaa
      361 gaatcatgag tgtccgctcc tcgctcaagg cggactgtta gacttgattg tatgctgtca
      421 gagaaaaccg tcagatcaat t
//
LOCUS       DAZKIP010000432          420 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZKIP010000432 DAZKIP010000000
VERSION     DAZKIP010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.437"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>420
                     /locus_tag="ACHOUK_005198"
     CDS             <1..>420
                     /locus_tag="ACHOUK_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900417.1"
                     /translation="LVKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMKF
                     VMNNPEGERADSPKVIIEISLSTITTMGTRQGHTAIIFPQPDGSTNRYERKSFERKDE
                     SSLHLITNKVLACYQREANKEIARLLNNHQKLNNLQK"
BASE COUNT          146 a           72 c           84 g          118 t
ORIGIN      
        1 ttttggtaaa aatatttacc ctgggttatg tggattttag caaacgctcc aacgaagcgg
       61 gaagaaatat gatggctcat attgagtcct catcttatat caaaaataat gatggcagtg
      121 agataatgaa gtttgttatg aataatcctg aaggggaacg agcggattca cccaaggtga
      181 ttatagaaat ttcactttcc actattacta ctatggggac tcgtcaagga catacagcca
      241 ttatatttcc acaacctgat ggttcgacta accgttatga aagaaagtcc tttgaaagaa
      301 aagatgagag ttcattacac ctgattacta acaaggttct ggcgtgttac caacgcgaag
      361 ctaacaagga aatagctcgt ctattaaata atcatcagaa gttaaataat ctacagaagt
//
LOCUS       DAZKIP010000433          419 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZKIP010000433 DAZKIP010000000
VERSION     DAZKIP010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.438"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(64..270)
                     /locus_tag="ACHOUK_005199"
     CDS             complement(64..270)
                     /locus_tag="ACHOUK_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFP9900418.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
BASE COUNT          112 a          102 c           83 g          122 t
ORIGIN      
        1 tcctgataca aaaaaccgcc agcgtttccg ctggcggttt tcaatgtatt caaaactcag
       61 caatcagtaa aaatcaccgg ttgccttttc ggcctgatcc atccaaaccg gtttatcgct
      121 ggttttcgac cagacgcgat gcaggtagct gtaaaagcgg gcgcggtctt tccagaaaag
      181 catgaaaggc agcgccagaa cgcccgctgc aaccgcataa gtacggcgta aaaagataag
      241 ccatgcagga tattctctgt agagttccat acttttctcc cccttttgta tctacccggt
      301 gaatagcacc ggaaaaatga atttgtttat ctgatgaaaa tagtaccgcc ttttgtgtaa
      361 ttttactact catccgacca cttatttttg cttattgatg gtttatttac attcatcct
//
LOCUS       DAZKIP010000434          418 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZKIP010000434 DAZKIP010000000
VERSION     DAZKIP010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.439"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>418
                     /locus_tag="ACHOUK_005200"
     CDS             <1..>418
                     /locus_tag="ACHOUK_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9900419.1"
                     /translation="SFFRLSHRPSWRYLGIGEEEARAFSREVEAAWKEFAEDDCCCID
                     VERKRTFTMMIREGVAMHAFNGELFVQATWDTRPSRLFRTQFRMVSPKRISNPNNTSD
                     SRNCRAGVQINDSGAALGYYVSEDGYPGWMPQKWTW"
BASE COUNT           90 a          109 c          131 g           88 t
ORIGIN      
        1 ggtctttttt ccggctcagt catcgcccaa gctggcgcta tctgggcatc ggggaggaag
       61 aagcccgtgc cttttcccgc gaggttgaag cggcatggaa agagtttgcc gaagatgact
      121 gttgctgcat tgacgttgag cgaaaacgca cgtttaccat gatgattcgg gaaggtgtgg
      181 ccatgcacgc ctttaacggt gaactgttcg ttcaggccac ctgggatacc cgtccctcgc
      241 gactgttccg gacacagttc cggatggtca gcccgaagcg catcagcaac ccgaacaata
      301 ccagcgacag ccggaactgc cgtgccggtg tgcagattaa tgacagcggt gcggcgctgg
      361 gatattacgt cagcgaggac gggtatcctg gctggatgcc gcagaaatgg acatggat
//
LOCUS       DAZKIP010000435          418 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZKIP010000435 DAZKIP010000000
VERSION     DAZKIP010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.440"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(278..>418)
                     /locus_tag="ACHOUK_005201"
     CDS             complement(278..>418)
                     /locus_tag="ACHOUK_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HFP9900420.1"
                     /translation="YRARETDGSASQTFDSGSQGQIQGKITGKDHRCAVGTKMQNGTF
                     CM"
BASE COUNT          108 a          108 c           77 g          125 t
ORIGIN      
        1 ttatgtaagg agtatttgat catgtataag caataaaaaa cagcttcagg taatgaggaa
       61 tatctcaatt tttaaacata aaatgcgaat tatttagtac aaaaagtgaa cttttgatga
      121 tctttcccta atctcacata ggatcagatc tcgtctgatc gttccccctg cgctcactac
      181 catggctctt taccacagca gccggggttg tttgggaccg acttccgaca gcagatcccg
      241 gtgggcctgc caccatctct tcacaagctt atgcttatta catgcaaaac gtcccgtttt
      301 gcatctttgt gcctacggca caccgatgat ctttccctgt gatctttccc tgaatctgcc
      361 cctgagaacc tgaatcaaac gtctgactcg cagaaccatc tgtttctcgc gctctgta
//
LOCUS       DAZKIP010000436          417 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZKIP010000436 DAZKIP010000000
VERSION     DAZKIP010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.441"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..>417)
                     /locus_tag="ACHOUK_005202"
     CDS             complement(<1..>417)
                     /locus_tag="ACHOUK_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900421.1"
                     /translation="TGYDSSLEGHSRLPDIKSVIAIAEIFSKRDEQLSSRDIFTTSVL
                     ALLMCAPSRISEILALPADCEITECDGKGIQRYGLRFFSAKGYEGNIKWIPTLMIPVA
                     KKAISRLKELSSQARLLAAEIQKNYSNSTKGTLKEN"
BASE COUNT          127 a           88 c           61 g          141 t
ORIGIN      
        1 atattttctt taagggttcc cttcgttgaa ttagagtaat tcttttgaat ttcagcagcc
       61 aataacctcg cttgacttga taattctttt aatctgctaa tagccttttt agctacaggt
      121 atcattaaag ttggaatcca ttttatattg ccttcatacc cttttgctga aaaaaatctt
      181 aaaccgtatc tttgaatgcc ctttccatca cattctgtga tttcacaatc cgctggcaaa
      241 gctaaaattt ccgatatcct actcggtgca cacataagta aagcaagcac agatgtagta
      301 aatatatctc ttgacgataa ttgttcatcc cgctttgaaa aaatctcagc aatcgcaata
      361 actgatttga tatcaggcaa tctagaatgg ccttccagtg aagaatcata accagtc
//
LOCUS       DAZKIP010000437          412 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZKIP010000437 DAZKIP010000000
VERSION     DAZKIP010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.442"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            15..412
                     /gene="tccP2"
                     /locus_tag="ACHOUK_005203"
                     /pseudo
     CDS             15..412
                     /gene="tccP2"
                     /locus_tag="ACHOUK_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_107686092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tir-cytoskeleton coupling protein TccP2"
BASE COUNT          125 a          115 c           68 g          104 t
ORIGIN      
        1 ataaggttat ctatatgata aatagcatta attctttttt tccggccttc ctcgcagtat
       61 atcaagtgct atacgcagca gtaccttcac tgtctcgcag cacaaaagca cacctaacac
      121 ggtaaaaacc agctcacctt tttctcccag caacagtcct gccagtgcaa caactatttt
      181 taaggtaaaa aattcttata cagaatcagg acttcaacgt cccacatctt atactcagtc
      241 aagtatagaa aaaaacgctt tacatcggcc tctacctgat gtagctcagc gtctggtgca
      301 gcatcttgca gagcatggca ttcaaccagc ccggagtatg gctgaacata ttcctccggc
      361 acctaactgg cctgcgccac caccgccagt acaaaatgaa caatcaagac ct
//
LOCUS       DAZKIP010000438          396 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZKIP010000438 DAZKIP010000000
VERSION     DAZKIP010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.443"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..165
                     /locus_tag="ACHOUK_005204"
     CDS             <1..165
                     /locus_tag="ACHOUK_005204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900422.1"
                     /translation="EEQAEWLIQCYRRRGCEVKKALSLDYRHWIISVRLPYSERPPRP
                     SRTFQQRIWR"
     gene            167..>396
                     /locus_tag="ACHOUK_005205"
     CDS             167..>396
                     /locus_tag="ACHOUK_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900423.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMSAFHNGRILVEPEPKSMRA
                     LPSGVVPAVHQPLAEDKSLLPFFSDERVIRAAG"
BASE COUNT           89 a          111 c          110 g           86 t
ORIGIN      
        1 gaagaacagg ccgaatggct cattcagtgt taccgcaggc gcggatgcga ggttaaaaaa
       61 gcccttagcc tcgactaccg tcactggata atctccgtca ggctccctta ctccgaacgg
      121 ccaccgcgtc cgtcccgcac attccagcaa cggatctgga ggtaatgtgc gggtattact
      181 tcgacctgtt ctggtaccgg aactcggtct ggttatcgtt aagccaggcc gtgaatcaat
      241 gtcagcattc cataacggca gaatactggt ggagccggaa ccaaaaagca tgcgagctct
      301 gccgtccggg gttgtacctg ccgttcacca gccgctggcg gaagataaat cactactgcc
      361 atttttcagc gatgagcggg tgatccgtgc tgcggg
//
LOCUS       DAZKIP010000439          382 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZKIP010000439 DAZKIP010000000
VERSION     DAZKIP010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.444"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            49..>382
                     /locus_tag="ACHOUK_005206"
     rRNA            49..>382
                     /locus_tag="ACHOUK_005206"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          112 a           79 c          116 g           75 t
ORIGIN      
        1 attttcgcaa cacgatgatg aatcgaaaga aacatcttcg ggttgtgagg ttaagcgact
       61 aagcgtacac ggtggatgcc ctggcagtca gaggcgatga aggacgtgct aatctgcgat
      121 aagcgtcggt aaggtgatat gaaccgttat aaccggcgat ttccgaatgg ggaaacccag
      181 tgtgattcgt cacactatca ttaactgaat ccataggtta atgaggcgaa ccgggggaac
      241 tgaaacatct aagtaccccg aggaaaagaa atcaaccgag attcccccag tagcggcgag
      301 cgaacgggga ggagcccaga gcctgaatca gtgtgtgtgt tagtggaagc gtctggaaag
      361 gcgcgcgata cagggtgaca gc
//
LOCUS       DAZKIP010000440          371 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZKIP010000440 DAZKIP010000000
VERSION     DAZKIP010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.445"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>371
                     /locus_tag="ACHOUK_005207"
     CDS             <1..>371
                     /locus_tag="ACHOUK_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFP9900424.1"
                     /translation="ALRNSVYLNGCNSLNESQCQFLAYDVSQGRACLSKAELTADLIW
                     LSANRTGEESAEELNYSGCDLSGLSLVGLNLSSVNFSGAVLDDTDLRMSDLSQAVLEN
                     CSFKNSILNECNFCYANLSNC"
BASE COUNT          102 a           58 c           82 g          129 t
ORIGIN      
        1 aggcactcag aaattccgta tatttaaatg gctgtaattc attaaatgaa tcgcaatgtc
       61 aattccttgc atatgatgtc agtcaaggcc gtgcctgcct gagcaaagct gagcttactg
      121 ctgacttaat ttggttgtca gctaaccgaa cgggtgaaga gtctgctgaa gaattgaatt
      181 actctggatg tgacttgtca ggtctaagtc ttgtagggct gaatttatca tcagtaaatt
      241 tttctggagc agtgcttgat gatacagatc tcaggatgag tgatttgtct caggctgtat
      301 tggaaaactg ttcttttaaa aactcgattt tgaatgaatg taatttttgt tatgctaatt
      361 tatctaattg t
//
LOCUS       DAZKIP010000441          368 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZKIP010000441 DAZKIP010000000
VERSION     DAZKIP010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.446"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>368
                     /locus_tag="ACHOUK_005208"
     CDS             <1..>368
                     /locus_tag="ACHOUK_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP9900425.1"
                     /translation="DWLKKEIQRVYDENHQVYGVRKVWRQLLREGIRVARCTVARLMA
                     VMGLAGVLRGKKVRTTVSRKAVAACDRVNRQFVAERPDQLWVADFTWVSTWQGFVYVA
                     FIIDVFAGYIVGWQVSSSMET"
BASE COUNT           84 a           82 c          115 g           87 t
ORIGIN      
        1 gactggctga agaaagagat acagcgcgta tacgatgaaa atcaccaggt atacggtgtg
       61 cgtaaagtct ggcgtcagtt gttacgggaa ggtatcagag tggccagatg cactgtggca
      121 cgtctcatgg cagttatggg acttgccggt gttctccggg gtaaaaaggt ccgcactacc
      181 gtcagccgga aagccgttgc cgcatgcgac cgcgtaaacc gtcagttcgt ggcagaacgt
      241 ccagaccagt tgtgggtggc tgattttacc tgggtaagca catggcaggg cttcgtttat
      301 gtggcgttca tcattgatgt gttcgccgga tacatcgtgg gatggcaggt ctcatcatcc
      361 atggaaac
//
LOCUS       DAZKIP010000442          367 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZKIP010000442 DAZKIP010000000
VERSION     DAZKIP010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.447"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..111
                     /locus_tag="ACHOUK_005209"
     CDS             <1..111
                     /locus_tag="ACHOUK_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP9900426.1"
                     /translation="RLHSELGMGFEERRVFRRYKGTGEQNTSGNARSKKC"
     gene            complement(193..>367)
                     /locus_tag="ACHOUK_005210"
     CDS             complement(193..>367)
                     /locus_tag="ACHOUK_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFP9900427.1"
                     /translation="PNQMIEQINIALDQKGSGNFSAWVIEACRRRLINEKHAQFVPNK
                     DKHDQSTCSDRFT"
BASE COUNT          108 a           61 c           85 g          113 t
ORIGIN      
        1 agactacatt ctgagttggg aatgggattt gaagagcggc gggtattccg acgatataaa
       61 gggacaggag aacagaatac gtccggaaac gcgcggtcaa aaaaatgcta aaaaatatct
      121 gagagcgtta ttgcctgtta ccataagaaa aagcgacttt agtggtcgct ttttgtgtca
      181 tatataagtc gtttaagtaa acctgtctga acaggtgctc tggtcgtgtt tgtctttgtt
      241 gggtacaaat tgagcatgtt tttcattaat taatcttctt ctgcaggctt caataaccca
      301 cgctgaaaaa ttacctgaac ctttctgatc aagagcgatg ttaatttgtt caatcatttg
      361 gttagga
//
LOCUS       DAZKIP010000443          366 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZKIP010000443 DAZKIP010000000
VERSION     DAZKIP010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.448"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            63..>366
                     /gene="rfbE"
                     /locus_tag="ACHOUK_005211"
                     /gene_synonym="perA"
     CDS             63..>366
                     /gene="rfbE"
                     /locus_tag="ACHOUK_005211"
                     /gene_synonym="perA"
                     /EC_number="2.6.1.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-perosamine synthase RfbE/PerA"
                     /protein_id="HFP9900428.1"
                     /translation="MKYIPVYQPSLTGKEKEYVNECLDSTWISSKGNYIQKFENKFAE
                     QNHVQYATTVSNGTVALHLALLALGISEGDEVIVPTLTYIASVNAIKYTGATPIFVD"
BASE COUNT          141 a           52 c           68 g          105 t
ORIGIN      
        1 atatggtata aatgtagttt taaaaaacat atcgatagac agttaaatat aagaggatga
       61 aaatgaaata tataccagtt taccaaccgt cattgacagg aaaagaaaaa gaatatgtaa
      121 atgaatgtct ggactcaacg tggatttcat caaaaggaaa ctatattcag aagtttgaaa
      181 ataaatttgc ggaacaaaac catgtgcaat atgcaactac tgtaagtaat ggaacggttg
      241 ctcttcattt agctttgtta gcgttaggta tatcggaagg agatgaagtt attgttccaa
      301 cactgacata tatagcatca gttaatgcta taaaatacac aggagccacc cccattttcg
      361 ttgatt
//
LOCUS       DAZKIP010000444          365 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZKIP010000444 DAZKIP010000000
VERSION     DAZKIP010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.449"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>365
                     /locus_tag="ACHOUK_005212"
     CDS             <1..>365
                     /locus_tag="ACHOUK_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900429.1"
                     /translation="SMITPFLTQRDFYIKEISKNEKVREQLVKDLKVRNQQEELLEKY
                     QVLRKDIEALTERLEALKKNAPSMEGILQGLGDRFSQYLKGINIKNRTGIKISESYYI
                     PVIREKEYFKITSGGLRTIS"
BASE COUNT          142 a           44 c           81 g           98 t
ORIGIN      
        1 tcaatgatta caccattctt gactcagaga gatttttata ttaaagagat ctcaaaaaat
       61 gagaaggtta gggagcagtt agtaaaagat ttgaaggtta gaaaccagca agaggaactg
      121 cttgaaaagt atcaggtact gagaaaagat attgaagcat taactgaacg cttggaggcg
      181 ttaaagaaaa acgcacctag catggagggc attttacaag gtttgggtga tagatttagt
      241 cagtatttga agggtattaa tatcaaaaac agaacgggga taaaaatctc tgaaagctat
      301 tacattcctg ttataagaga aaaagaatat tttaaaataa catccggagg tttgagaact
      361 atttc
//
LOCUS       DAZKIP010000445          361 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZKIP010000445 DAZKIP010000000
VERSION     DAZKIP010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.450"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..>361)
                     /locus_tag="ACHOUK_005213"
     CDS             complement(<1..>361)
                     /locus_tag="ACHOUK_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900430.1"
                     /translation="PPYGTRSYKDTFQLIFYKNNIRYYKPAIYYGVAQSNIRLHEDSD
                     KHCITFLARDKKTQKPFTLEIDVSDWNKSQKDVFWKEYEKQRKEADRYYKGLKDKRNA
                     KKYLTVFFFGMPDENDK"
BASE COUNT          109 a           70 c           53 g          129 t
ORIGIN      
        1 aacttatcat tttcatccgg catcccaaaa aagaaaaccg tcaagtattt tttagcattc
       61 cttttatctt taagcccctt ataataccta tcagcttcct tcctttgctt ttcatattcc
      121 ttccaaaaca catccttctg acttttattc cagtcagaaa catcaatttc aagcgtaaat
      181 ggtttttggg ttttcttatc ccttgcgagg aaagtaatac aatgtttatc tgaatcttca
      241 tgcagacgaa tattagactg ggctacacca taataaatag caggtttgta ataacgtatg
      301 ttgtttttgt aaaaaataag ttgaaatgta tctttataag atcgcgtgcc atatggaggg
      361 a
//
LOCUS       DAZKIP010000446          355 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZKIP010000446 DAZKIP010000000
VERSION     DAZKIP010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.451"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..147
                     /locus_tag="ACHOUK_005214"
     CDS             <1..147
                     /locus_tag="ACHOUK_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFP9900431.1"
                     /translation="GNEETNLWTLCTECHRQKSAREAAGGMPDPTLPEVSGGHGRADD
                     IIGL"
BASE COUNT           83 a           87 c          103 g           82 t
ORIGIN      
        1 ggtaatgagg agacgaatct ctggacgctc tgtaccgaat gccatcgaca aaagtctgct
       61 cgtgaagcgg cgggtggtat gccggacccg acgctgccgg aggtgtccgg aggtcatggc
      121 agggcagacg atatcatcgg actgtgaccc gccccggggg ggggatcatc cggcgaaaaa
      181 aacgatcgct ccggacaccg cgccccctct cacgcagaga aaaaattccc gtttcagggc
      241 agttaacatg ttaactggct gtccgggcat ttttgcggtt tttatcttta ttattcagtt
      301 tgttgtgcgg aaaaaatgtt aactggcttt ttcagcaaat gttaaccagg cagca
//
LOCUS       DAZKIP010000447          348 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZKIP010000447 DAZKIP010000000
VERSION     DAZKIP010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.452"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..201
                     /locus_tag="ACHOUK_005215"
     CDS             <1..201
                     /locus_tag="ACHOUK_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900432.1"
                     /translation="LFNPDTVEAYAKRAEISPKPLFSAPPALVVPDKLPREYRNGWPL
                     AYSDYAEGWNDCREAMLQGDKS"
     gene            198..>348
                     /locus_tag="ACHOUK_005216"
     CDS             198..>348
                     /locus_tag="ACHOUK_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900433.1"
                     /translation="MINRIKLEHILEYARQQRHIGQHCKIPPGDMVEIMEIAMRKAGN
                     SPVTPD"
BASE COUNT           97 a           83 c           89 g           79 t
ORIGIN      
        1 ctgtttaacc ctgacacagt tgaagcatac gcaaaacgtg cggaaatatc acctaaacca
       61 ctattctccg cgccgccagc gctggtagtg cctgataagt tgccgcgtga atacagaaac
      121 ggttggcctc ttgcgtatag tgattatgct gaaggctgga acgactgccg cgaagccatg
      181 cttcagggag ataaatcatg attaatcgta tcaagctgga gcacatcctc gaatatgcca
      241 ggcagcagag gcatattggt cagcattgta aaattccacc aggagatatg gttgaaatca
      301 tggagattgc catgcgcaag gctggcaact ctccggtaac tccggatg
//
LOCUS       DAZKIP010000448          339 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZKIP010000448 DAZKIP010000000
VERSION     DAZKIP010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.453"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>339
                     /locus_tag="ACHOUK_005217"
     CDS             <1..>339
                     /locus_tag="ACHOUK_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFP9900434.1"
                     /translation="VFHTRIQHGEPQVESRYLYDPLGRRTGKRVWRRERDLTGWMSLS
                     RKPEETWYGWDGDRLTTVQTQQTRIQTVYQPGSFTPLLRIETENGEQAKARHRSLAEV
                     LQEDTGVTLPA"
BASE COUNT           79 a           89 c          126 g           45 t
ORIGIN      
        1 ggtgttccac acgcggatac agcacggcga accacaggtg gagagccggt acctctatga
       61 cccgctgggc cgccggacgg gaaaacgggt gtggcggcgg gagcgtgacc tgacggggtg
      121 gatgtcgctg tcgcgtaaac cggaggagac ctggtacggg tgggacggtg acaggctgac
      181 cactgtacag acccaacaga caagaatcca gacggtatac cagccgggaa gcttcacgcc
      241 gctcctgaga atcgaaacag agaatggtga acaggcgaag gcgcggcacc gtagcctggc
      301 ggaggtgttg caggaggaca cgggtgtgac gctaccggc
//
LOCUS       DAZKIP010000449          337 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZKIP010000449 DAZKIP010000000
VERSION     DAZKIP010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.454"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..75
                     /locus_tag="ACHOUK_005218"
     CDS             <1..75
                     /locus_tag="ACHOUK_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
                     /protein_id="HFP9900435.1"
                     /translation="DVLITYRGKLPGIITGSLKTPPLA"
     gene            126..>337
                     /locus_tag="ACHOUK_005219"
     CDS             126..>337
                     /locus_tag="ACHOUK_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP9900436.1"
                     /translation="MNAKIRYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLS"
BASE COUNT           90 a           80 c           92 g           75 t
ORIGIN      
        1 gatgtgttga taacctacag aggaaaacta ccgggaataa ttacgggttc tctgaagact
       61 ccaccgctgg cctgaagact taacatatcc agggatttga aatcgataaa ccctgataaa
      121 tatccatgaa cgcaaaaatc agatacggcc tgtcggctgc cgttctggcg ctgattggtg
      181 caggggcgtc tgcgcctgaa atcctcgacc agtttctgga tgaaaaggaa ggtaaccaca
      241 ccacagcata ccgtgatggt gcgggtatct ggaccatctg ccgtggagcc acccgggtgg
      301 atggtaagcc tgtgattcct ggcatgaagc tgtcgaa
//
LOCUS       DAZKIP010000450          337 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZKIP010000450 DAZKIP010000000
VERSION     DAZKIP010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.455"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..>337)
                     /locus_tag="ACHOUK_005220"
     CDS             complement(<1..>337)
                     /locus_tag="ACHOUK_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP9900437.1"
                     /translation="SKAIEMLRDPDWVSKITHLDQDVLNVLLNGKVKFISEKYNTRYS
                     INYELKDKVDNPVNDDTVFIHYVGPTKPWHEWADYPVSRSFLIAKAASPWSKEDLLKP
                     VNSNQYRYCA"
BASE COUNT           99 a           71 c           55 g          112 t
ORIGIN      
        1 tgcacaatac cgatactgat tgctatttac aggtttaagt aaatcttctt tactccacgg
       61 agaagctgct ttggcgatca aaaaactacg tgacaccgga tagtcagccc actcatgcca
      121 aggttttgta ggtcccacat agtgtataaa cacggtgtca tcattgactg gattatcaac
      181 tttgtctttt aattcatagt taatactata tcgggtatta tatttctccg aaataaactt
      241 cactttacca ttcaataata cattcagtac atcttgatca aggtgggtga ttttacttac
      301 ccaatctggg tcacgcaaca tttcaatagc ttttgac
//
LOCUS       DAZKIP010000451          333 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZKIP010000451 DAZKIP010000000
VERSION     DAZKIP010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.456"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>333
                     /locus_tag="ACHOUK_005221"
     CDS             <1..>333
                     /locus_tag="ACHOUK_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFP9900438.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAK"
BASE COUNT           74 a           85 c          103 g           71 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaa
//
LOCUS       DAZKIP010000452          312 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZKIP010000452 DAZKIP010000000
VERSION     DAZKIP010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.458"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            74..289
                     /locus_tag="ACHOUK_005222"
     CDS             74..289
                     /locus_tag="ACHOUK_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFP9900439.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
BASE COUNT           94 a           73 c           73 g           72 t
ORIGIN      
        1 caacagaatg catttttctg gcagcaggct tcatattctg tgtgcttatg cttgccgaca
       61 tgggacttgt tcaatgacac ctcagcagga aaacgccctt cgcagcattg cccgtcaggc
      121 taattctgaa atcaaaaaag ccagacagca gtttccggat aaaaacgtcg atgacatttg
      181 ccgtagcgta ctgaagaagc accgcgaaac ggtaacgcta atgggattca caccgactca
      241 tttaagtctg gcgatcggca tgttaaacgg cgtctttaag gagcgatgaa catgaaaagc
      301 aaaatcatca gg
//
LOCUS       DAZKIP010000453          296 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZKIP010000453 DAZKIP010000000
VERSION     DAZKIP010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.459"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..150)
                     /gene="cesF"
                     /locus_tag="ACHOUK_005223"
     CDS             complement(<1..150)
                     /gene="cesF"
                     /locus_tag="ACHOUK_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesF"
                     /protein_id="HFP9900440.1"
                     /translation="MNEKFRTDLAHTFGIALEEQTDVLSFHDNDGHEWILECASQSEI
                     LFFYCY"
BASE COUNT           99 a           53 c           55 g           89 t
ORIGIN      
        1 ataacaataa aaaaacagaa tttcagattg agatgcacac tccagtatcc attcatggcc
       61 atcgttatcg tggaaagaga gcacgtcagt ttgctcttca agagctatcc caaacgtatg
      121 tgcaaggtct gtgcgaaatt tctcattcat attgtagtcc tgccattctt ttcatgttga
      181 tatactcaaa cgtaattaat ccgagagttc tgatacaaat aataaggcgt agatataaga
      241 ttacgggaat agtgttgcat attcatgata gcatatgata aatgtagtgc aactac
//
LOCUS       DAZKIP010000454          287 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZKIP010000454 DAZKIP010000000
VERSION     DAZKIP010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.460"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
BASE COUNT          105 a           39 c           57 g           86 t
ORIGIN      
        1 cgcatattag caaaaaaaat attattttag gtatttctac agtacatttg acacgatgat
       61 taacctctat gtagctaggt ttttgtaaag aaactgtgcg tcactgtatc caggactgtt
      121 atgtgccaaa taaggaagtt gaagttacca gctcttactc taaagctata acgtatagaa
      181 aaaatgcatt tgagacgtgt aaaatcattt atttaatcgc aatttatgta atggcataat
      241 caggtcggtg aaagagggga gaagaatgga taaaagagct aaaaatc
//
LOCUS       DAZKIP010000455          286 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZKIP010000455 DAZKIP010000000
VERSION     DAZKIP010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.denovo.461"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>286
                     /locus_tag="ACHOUK_005224"
     CDS             <1..>286
                     /locus_tag="ACHOUK_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9900441.1"
                     /translation="GQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNP
                     AWCLWDMLTHPRYGMGKRLGAADVDKWALYAIAQYCDQMVPDGFGGTEPRM"
BASE COUNT           66 a           81 c           94 g           45 t
ORIGIN      
        1 ggccagcaga tgacggtgaa ctaccatatc cgaggtcgca tcatccaggt gccgtcaaac
       61 tatgacccgg aaaaacgcac gtacagcggc atctgggacg gcagcctgaa accggcatac
      121 agcaacaacc cggcctggtg cctgtgggac atgctgactc acccgcgcta cggcatggga
      181 aaacgcctgg gggcggcgga tgtggacaag tgggcgctgt atgccattgc gcagtactgc
      241 gaccagatgg tccctgatgg tttcgggggc acagagccgc ggatga
//
LOCUS       DAZKIP010000456        16919 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.guided.093, whole genome shotgun sequence.
ACCESSION   DAZKIP010000456 DAZKIP010000000
VERSION     DAZKIP010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16919
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.guided.093"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(184..1161)
                     /gene="epmA"
                     /locus_tag="ACHOUK_005225"
     CDS             complement(184..1161)
                     /gene="epmA"
                     /locus_tag="ACHOUK_005225"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFP9900442.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1486..3294
                     /gene="frdA"
                     /locus_tag="ACHOUK_005226"
     CDS             1486..3294
                     /gene="frdA"
                     /locus_tag="ACHOUK_005226"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFP9900443.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTKRDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3287..4021
                     /gene="frdB"
                     /locus_tag="ACHOUK_005227"
     CDS             3287..4021
                     /gene="frdB"
                     /locus_tag="ACHOUK_005227"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFP9900444.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4032..4427
                     /gene="frdC"
                     /locus_tag="ACHOUK_005228"
     CDS             4032..4427
                     /gene="frdC"
                     /locus_tag="ACHOUK_005228"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFP9900445.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4438..4797
                     /gene="frdD"
                     /locus_tag="ACHOUK_005229"
     CDS             4438..4797
                     /gene="frdD"
                     /locus_tag="ACHOUK_005229"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFP9900446.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            4860..5993
                     /locus_tag="ACHOUK_005230"
     CDS             4860..5993
                     /locus_tag="ACHOUK_005230"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFP9900447.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIVNGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            6081..6614
                     /gene="blc"
                     /locus_tag="ACHOUK_005231"
     CDS             6081..6614
                     /gene="blc"
                     /locus_tag="ACHOUK_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFP9900448.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6611..6928)
                     /gene="sugE"
                     /locus_tag="ACHOUK_005232"
     CDS             complement(6611..6928)
                     /gene="sugE"
                     /locus_tag="ACHOUK_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFP9900449.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7104..7250)
                     /gene="ecnB"
                     /locus_tag="ACHOUK_005233"
     CDS             complement(7104..7250)
                     /gene="ecnB"
                     /locus_tag="ACHOUK_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFP9900450.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7361..7486)
                     /gene="ecnA"
                     /locus_tag="ACHOUK_005234"
     CDS             complement(7361..7486)
                     /gene="ecnA"
                     /locus_tag="ACHOUK_005234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFP9900451.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7538..8104)
                     /gene="efp"
                     /locus_tag="ACHOUK_005235"
     CDS             complement(7538..8104)
                     /gene="efp"
                     /locus_tag="ACHOUK_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFP9900452.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8146..9174
                     /gene="epmB"
                     /locus_tag="ACHOUK_005236"
     CDS             8146..9174
                     /gene="epmB"
                     /locus_tag="ACHOUK_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFP9900453.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RNAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRAGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            9409..10278
                     /locus_tag="ACHOUK_005237"
     CDS             9409..10278
                     /locus_tag="ACHOUK_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFP9900454.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSP
                     LDFLPLYDVDCQQNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLVGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(10470..10823)
                     /locus_tag="ACHOUK_005238"
     CDS             complement(10470..10823)
                     /locus_tag="ACHOUK_005238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFP9900455.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(10961..12607)
                     /gene="groL"
                     /locus_tag="ACHOUK_005239"
     CDS             complement(10961..12607)
                     /gene="groL"
                     /locus_tag="ACHOUK_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFP9900456.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(12651..12944)
                     /locus_tag="ACHOUK_005240"
     CDS             complement(12651..12944)
                     /locus_tag="ACHOUK_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFP9900457.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            13220..14476
                     /gene="yjeH"
                     /locus_tag="ACHOUK_005241"
     CDS             13220..14476
                     /gene="yjeH"
                     /locus_tag="ACHOUK_005241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFP9900458.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPILIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(14492..14968)
                     /locus_tag="ACHOUK_005242"
     CDS             complement(14492..14968)
                     /locus_tag="ACHOUK_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFP9900459.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            15305..16741
                     /gene="aspA"
                     /locus_tag="ACHOUK_005243"
     CDS             15305..16741
                     /gene="aspA"
                     /locus_tag="ACHOUK_005243"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFP9900460.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
BASE COUNT         4241 a         4323 c         4221 g         4134 t
ORIGIN      
        1 cgcccactga actgcactgc ccaacattga gcgggcagcg taaaaaccaa tacagaattg
       61 atacctggat gatatcaaat atccagtctt aaattgtggc ctttagcgga aaaatgaaaa
      121 catatgctac agaatggcgc ttatcacgcc attcttcgct gttaattcag taatttttca
      181 gaattatgcc cggtcaacgc taaaggcgat gacttcagcc agtgtctccg cgcccagcgc
      241 caacatcacc agacgatcaa cacctaatgc cacgccggaa cagtcaggca taccgacttt
      301 caaggcttca atcagattct gatcaatggg gtgctgcggc aaaccgcgcg ccgcgcgctt
      361 acggttatct tgttcaaagc gttgttgctg ctcacgggca tccgtcaatt catggaaacc
      421 attcgccagc tcaatacctt tataataaac ctcaaagcgt tcagcgaccc gatgatcttc
      481 ggtactgatt tgcgccagtg atgcctggct ggctggaaag tggtacacaa aggtcggttt
      541 ttctttacca atatttggtt ctacgccaaa ggtaaacagc aattgtagca acgtgtcgcg
      601 gtcttcttcg gtatcagcaa cattgctcaa atccagtttc gctgccactt cccgcagttg
      661 cgttttgtcg gcagagagcg ggtcaatttc cagataacgc aagaaagctt gttgataaga
      721 aaggctttct gctgccgggc agtccagcac ctgttgtaag agatcgtcca cctcgttcat
      781 caaccggtac atatcatagt gcggtcgata ccactcaagc atagtgaact cagggttgtg
      841 ataacgcccc atctcttcat tacggaaact gcggcacagc tggaataccg gcccgcaacc
      901 ggcaaccagc aggcgtttca tatggtattc cgggctggtc attaaccaga gattcatccc
      961 ctgcgaatgc ccggggccaa cgaaacgtgt ctcaaacggg accaaatgaa tatcggttac
     1021 cgtcgcctgg ctcatacaag gcgtctccac ctccagcact ccacgatcgg caaagaaacg
     1081 acggatctcc gccataatcg ccgcgcgttt taataagtta ggaatggatg cgctcggctg
     1141 ccaggatgcc gtttcgctca tagttaaatc tccagttttt gacaagggca cgaagtctac
     1201 tcgcaacgcg acggcgagac aaattttacg caggaataaa acaacggtgg gcagtgacta
     1261 aaaaaagcac gatctgatgg tttagtaatt aaattaatca tcttcagtga taatttagcc
     1321 ctcttgcgca ctaaaaaaat cgatctcgtc aaatttcaga cttatccatc agactatact
     1381 gttgtaccta taaaggagca gtggaatagc gttcgcagac cgtaactttc aggcacttac
     1441 cctgaagtac ggggctgtgg gataaaaaca atctggagga atgtcgtgca aacctttcaa
     1501 gccgatcttg ccattgtagg cgccggtggc gcgggattac gtgctgcaat tgctgccgcg
     1561 caggcaaatc caaatgcaaa aatcgcacta atctcaaaag tatacccgat gcgtagccat
     1621 accgttgctg cagaaggggg ctccgccgct gtcgcgcagg atcatgacag cttcgaatat
     1681 cactttcacg atacagtagc gggtggcgac tggttgtgtg agcaggatgt cgtggattat
     1741 ttcgtccacc actgcccaac cgaaatgacc caactggaac tgtgggggtg cccatggagc
     1801 cgtcgcccgg atggtagcgt caacgtacgt cgcttcggcg gcatgaaaat cgagcgtacc
     1861 tggttcgccg ccgataagac cggcttccat atgctgcaca cgctgttcca gacctctctg
     1921 caattcccgc agatccagcg ttttgacgaa catttcgtgc tggatattct ggttgatgat
     1981 ggtcatgttc gcggcctggt agcaatgaac atgatggaag gcacgctggt gcagatccgt
     2041 gctaacgcgg tcgttatggc tactggcggt gcgggtcgcg tttatcgtta caacaccaac
     2101 ggcggcatcg ttaccggtga cggtatgggt atggcgctaa gccacggcgt tccgctgcgt
     2161 gacatggaat tcgttcagta tcatccaacc ggtctgccag gttccggtat cctgatgacc
     2221 gaaggttgcc gcggtgaagg cggtattctg gtcaacaaaa atggctaccg ttatctgcaa
     2281 gattacggca tgggcccgga aactccgctg ggcgagccga aaaacaaata tatggaactg
     2341 ggtccacgcg acaaagtttc tcaggccttc tggcacgaat ggcgtaaagg caacaccatc
     2401 tccacgccgc gtggtgatgt ggtttatctc gacctgcgtc acctcggcga gaaaaaactg
     2461 catgaacgtc tgccgttcat ctgcgaactg gcgaaagcgt acgttggcgt cgatccggtt
     2521 aaagaaccga ttccggtacg tccgaccgca cactacacca tgggcggtat cgaaaccgat
     2581 cagaactgtg aaacccgcat taaaggtctg ttcgccgtgg gtgaatgttc ctctgttggt
     2641 ctgcacggtg caaaccgtct gggctccaac tccctggcgg aactggtggt cttcggccgt
     2701 ctggccggtg aacaagcgac agagcgtgca gcaactgccg gtaatggcaa cgaagcggca
     2761 attgaagcgc aggcagctgg cgttgaacaa cgtctgaaag atctggttaa ccaggatggc
     2821 ggcgaaaact gggcgaagat ccgcgacgaa atgggcctgg ctatggaaga aggttgcggt
     2881 atctaccgta cgccggaact gatgcagaaa accatcgaca agctggcaga gctgcaggaa
     2941 cgcttcaagc gcgtgcgcat caccgacact tccagcgtgt tcaacaccga cctgctctac
     3001 accattgaac tgggccacgg tctgaacgtt gctgaatgta tggcgcactc cgcaatggca
     3061 cgtaaagagt cccgcggcgc acaccagcgt ctggacgaag gttgcaccaa gcgtgacgac
     3121 gtcaacttcc tcaaacacac cctcgccttc cgcgatgctg atggcacgac tcgcctggag
     3181 tacagcgacg tgaagattac tacgctaccg ccagctaaac gcgtttacgg tggcgaagcg
     3241 gatgcagccg ataaggcgga agcagccaat aagaaggaga aggcgaatgg ctgagatgaa
     3301 aaacctgaaa attgaggtgg tgcgctataa cccggaagtc gataccgcac cgcatagcgc
     3361 attctatgaa gtgccttatg acgcaactac ctcattactg gatgcgctgg gctacatcaa
     3421 agacaacctg gcaccggacc tgagctaccg ctggtcctgc cgtatggcga tttgtggttc
     3481 ctgcggcatg atggttaaca acgtgccaaa actggcatgt aaaaccttcc tgcgtgatta
     3541 caccgacggt atgaaggttg aagcgttagc taacttcccg attgaacgcg atctggtggt
     3601 cgatatgacc cacttcatcg aaagtctgga agcgatcaaa ccgtacatca tcggcaactc
     3661 ccgcaccgcg gatcagggta ctaacatcca gaccccggcg cagatggcga agtatcacca
     3721 gttctccggt tgcatcaact gtggtctgtg ctacgccgcg tgcccgcagt ttggcctgaa
     3781 cccagagttc atcggtccgg ctgccattac gctggcgcat cgttataacg aagatagccg
     3841 cgaccacggt aagaaggagc gtatggcgca gttgaacagc cagaacggcg tatggagctg
     3901 tactttcgtg ggctactgct ccgaagtctg cccgaaacac gtcgatccgg ctgcggccat
     3961 tcaacagggc aaagtagaaa gttcgaaaga ctttcttatc gcgaccctga aaccacgcta
     4021 aggagtgcaa catgacgact aaacgtaaac cgtatgtacg gccaatgacg tccacctggt
     4081 ggaaaaaatt gccgttttat cgcttttaca tgctgcgcga aggcacggcg gttccggctg
     4141 tgtggttcag cattgaactg attttcgggc tgtttgccct gaaaaatggc ccggaagcct
     4201 gggcgggatt cgtcgacttt ttacaaaacc cggttattgt gatcattaac ctgatcactc
     4261 tggcggcagc tctgctgcac accaaaacct ggtttgaact ggcaccgaaa gcggccaata
     4321 tcattgtaaa agacgaaaaa atgggaccag agccaattat caaaagtctc tgggcggtaa
     4381 ctgtggttgc caccatcgta atcctgtttg ttgccctgta ctggtaagga gcctgagatg
     4441 attaatccaa atccaaagcg ttctgacgaa ccggtattct ggggcctctt cggggccggt
     4501 ggtatgtgga gcgcaattat tgcgccggtg atgatcctgc tggtgggtat tctgctgccg
     4561 ctggggctgt ttccgggtga tgcactgagc tacgagcgcg ttctggcgtt cgcgcagagc
     4621 ttcattggtc gcgtattcct gttcctgatg atcgttctgc cgctgtggtg tggtttacac
     4681 cgtatgcacc acgcgatgca cgatctgaaa atccacgtac ctgcgggcaa atgggttttc
     4741 tacggtctgg ctgctatcct gacagttgtc acgctgattg gtgtcgttac aatctaacgc
     4801 atcgccaatg taaatccggc ccgcctatgg cgggccgttt tgtatggaaa ccagacccta
     4861 tgttcaaaac gacgctctgc gccttattaa ttaccgcctc ttgctccaca tttgctgccc
     4921 ctcaacaaat caacgatatt gtgcatcgca caattacccc gcttatagag caacaaaaga
     4981 tccccggtat ggcggtggcg gtaatttatc agggtaaacc ttattacttt acctgggggt
     5041 atgcggacat cgccaaaaag cagcccgtca cacagcaaac gttgtttgag ttaggttcgg
     5101 tcagcaaaac atttacgggc gtgcttggtg gcgacgctat tgctcgaggg gaaatcaagt
     5161 taagcgatcc cgcaacaaaa tactggcctg aacttaccgc taaacagtgg aatgggatca
     5221 cactattaca tctcgcaacc tacactgctg gcggcctgcc attgcaggtg ccggatgaag
     5281 tgaaatcctc aagcgacttg ctgcgcttct atcaaaactg gcagcctgca tgggctccag
     5341 gaacacaacg tctgtatgcc aactccagta tcggtttgtt cggcgcactg gctgtgaagc
     5401 cgtctggttt gagttttgag caggcgatgc aaactcgtgt cttccagcca ctcaaactca
     5461 accatacgtg gattaatgta ccgcccgcag aagaaaagaa ttacgcctgg ggatatcgcg
     5521 aaggcaaggc agttcatgtt tcgccagggg cgttagatgc tgaaacttat ggtgtgaagt
     5581 cgaccattga agatatggcc tgctgggtac gaagcaatat gaatccccgt gatatcaacg
     5641 acaaaacact tcagcaaggg atacaactgg cacaatctcg ctactggcaa accggcgata
     5701 tgtatcaggg tctgggctgg gaaatgctgg actggccggt aaatcctgac atcatcgtta
     5761 acggcagtga caataaaatt gcactggcag cacgccccgt aaaagcgatt acgcctccaa
     5821 ctcctgcagt acgagcatca tgggtgcata aaacgggggc gaccggcgga tttggtagct
     5881 atgtcgcgtt tattccagaa aaagagctgg gtatcgtgat gctggctaac aaaaactatc
     5941 ccaatccagc gagagtcgcc gccgcctggc agattctcaa cgctctacag taaaatttca
     6001 tcgggtccga attttcggac cttttctgcg cttttccttg ctgtcatcta cacttagaaa
     6061 aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg acagctgcat
     6121 ttctggtcgt tgcctgcagt tctcctacgc cgccgcgtgg cgtgaccgta gtgaataatt
     6181 tcgacgctaa acgctatctc ggtacctggt atgagattgc ccgttttgat caccgctttg
     6241 aacgtggact ggaaaaagtc accgcaacat acagcctgcg tgatgacggc ggcctgaatg
     6301 tcattaataa aggctataac cctgacagag gaatgtggca gcagagtgaa gggaaagcgt
     6361 actttaccgg cgcaccaact cgcgctgcgc tgaaagtgtc attctttggt cctttctatg
     6421 gcggttataa cgttattgca ctcgatcggg aataccgcca tgcgctggtt tgcggaccgg
     6481 accgcgacta cctgtggata ctctcccgca cgccaaccat ttctgacgaa gtgaaacagg
     6541 agatgctggc agtcgcgacc cgggaagggt ttgatgtcag taagtttatt tgggtacagc
     6601 agcccggtag ttagtgagtg ctgagtttca gaccaataat ccccaatacg attaacgcca
     6661 gactcgccag acgcatcggg ttagcggact caccgagcag cacaatgccg gtgatggccg
     6721 cgccgactgc gccaataccc gtccacacgg cataagccgt ccctactggt aacgatttca
     6781 tcgcccaggc aagtagcgcc atactgacaa tcatcgccgt cacggtaata acactcggcg
     6841 tcaaacgact aaagccgtgg gtatatttca ggcctacggc ccataccact tccagcagac
     6901 cagcaataac taagataatc caggacatat caggctccgg agaattgggg ccgtccccgg
     6961 tgaaaagacg cgtttgcagg tcgtcctgca aagctgatgg ttgaacggat attttgccag
     7021 gtggcggggg aatttcaact attacctttg tgactatcgc agaaaaaaca aacggcacga
     7081 cacagctgtc gtaccgttat tgcttattgc tgcgctttcg ttgctgcacc agaaatcgcg
     7141 ttaccgccat cagaaatgtc ttcaccaacg ccacgcgtgg tgttgcaggc agttaatact
     7201 gttgaaagca ccagaacaga aaagatcgct gcaattgtct tcttcaccat aacgtcttcc
     7261 ttttaggtaa tgttatttat gtttgcctat agcaaattaa taatagacaa catcgccaaa
     7321 aatgatggaa atctgatgat ttttcggaag agaagaaaaa ttagctggca gcgcgggaga
     7381 tggagttgcc gagatgtttg atgtcttcgc cgaaaccgcg agcggtgtta cagcccgtga
     7441 gcagcgtgct ggcaagcaaa acaagaacga taaggcgttt catcatctct gacctgaata
     7501 agtgatggtg cagcctgcag gccgcaccac aaccgcatta cttcacgcga gagacgtatt
     7561 caccagagcg ggtatccact ttgatgactt cgccgatttg tacgaacagc ggaactttaa
     7621 ccacagcgcc agtagacagg gtagccggtt tgccgccagt acctgcggta tcacctttca
     7681 ggcccggatc ggtatcaacg atttccagtt caacgaagtt cggcggagta acggagatcg
     7741 gctgaccatt ccacagagtt acgatacact ctgcctgatc cagcagccat ttagcgttgt
     7801 cgccaattgc ttttgcgtca gcagacagct gctcgaaagt ttcgttgttc atgaagtgcc
     7861 agaactcacc gtcgttgtac aggtaagtca ggttcatatc gacaacatca gcgccttcag
     7921 cggaatcagt agatttgaag gttttttcta cgcgagtacc ggtcagcaga cgacgcagtt
     7981 taacgcgagc aaatgcctgg cctttacccg gttttacgaa ttcactcgct tcaaccgcgt
     8041 aaggttcgcc gtctaacatg attttaagac cagcacgaaa atcgttgcta tagtacgttg
     8101 ccataaggcc ctctgaaatt tgttaattgg tagctaagcc acaaaatggc gcatattgta
     8161 accctaaata ccccatccag agaagattgg ttaacgcaac ttgccgatgt tgtgaccgat
     8221 cctgatgaac ttctgcgtct tttgaatata gacgcggacg aaaaactgtt ggccggacgc
     8281 aacgccaaaa agctgtttgc cctgcgtgtg ccccgctcat ttatcgatcg catggagaaa
     8341 ggcaatccgg acgatccgct tttgcgtcag gtacttacct cgcaagatga gtttgtcgtc
     8401 gcgcccggat tctccaccga cccgctggaa gaacagcaca gcgtagtgcc tggtttgttg
     8461 cataaatacc acaaccgggc gcttttgctg gtcaaaggcg gctgcgcggt aaattgccgc
     8521 tattgcttcc gtcgccactt cccctatgcc gaaaatcagg gcaacaagcg taactggcaa
     8581 actgcacttg agtatgttgc tgcgcatccg gaactggacg agatgatttt ctccggcggc
     8641 gatccgctga tggcgaaaga tcacgagctg gactggttgc tcacacaact ggaagccatc
     8701 ccgcatatca aacgtctgcg gattcacagt cgtctgccga ttgtgatccc ggcacgcatc
     8761 acagatgcgc tggttgaacg cttttcacat tctacgctgc aaatcttgct ggtgaatcac
     8821 atcaaccatg ccaatgagat agatgaaaca ttccgtcagg cgatggctaa gttgcgccgg
     8881 gcgggcgtta ctttgctgaa ccagagcgtt ctgttacgtg gtgtgaacga taacgcacaa
     8941 acgctggcaa acctgagtaa tgcgttgttc gatgcaggcg tgatgccgta ttacctgcat
     9001 gtgctcgata aagtacaggg cgcggcgcat tttatggtga gtgatgacga agcacggcag
     9061 attatgcgtg agttgctgac actggtgtcg ggatatctgg tgccgaaact ggcgcgagaa
     9121 attggcggcg aacccagcaa aacgccgctg gatctccagc tacgccagca gtaactgatt
     9181 aaacagggaa gtttaatctt tgcccgttac ataaccttta attattcata tacattgctg
     9241 ttgttactct ttatataacc aacaccaata attaaccttt cttttctgtt tatatagagt
     9301 gagtacggct atttatgtta ttttaacaac tattactacc aagcccattt atctggctta
     9361 taaattaaaa ttaattattt aattgagaaa tggttaggga gaacctacat ggccataagc
     9421 atcaagggtg ttaataccgg tgttattcgc aagagtaata atttcattgc actggcgctg
     9481 aaaattaaag aacctcgcaa taaagaatcg ttgttcttta tgtctgtcat ggaactgcgc
     9541 gacctgctga ttgcgctgga aagtcgtctg catcaaaagc ataaactgga tgctgctgca
     9601 cgccttcagt atgagcaagc tcgcgataaa gtcattaaga aaatggcaga aaacatccca
     9661 gaaattctgg ttgatgagct taaaaatgcc gatattaatc gtcgggttaa cacgctggaa
     9721 cttaccgata atcaaggcga aaatcttacc ttcgtattaa cgctacatga cggtagcaaa
     9781 tgtgagttag tcgtcaatga gttgcagatt gaaatgctgg cgcgagcaat cattcatgca
     9841 attaataatg cggaaatgcg tgaactggca ttacgtatta cctcaccgtt agatttcctg
     9901 ccgttatatg acgttgactg ccagcaaaat ggcaatctgg aatatgacac ctattcccaa
     9961 ccggaatgga aacataactt gttcgaccat tatctggcgg tgctttaccg ctttaaagat
    10021 gaaagtggca aagaacaatt tagcggcgca gtggtgaaaa cgcgtgaagc aacgccgggt
    10081 aaagaaatag aggcgatcac tcgccgaatg cttgatttca gcccacgact gaaaaaactc
    10141 gtgggtgtgc catgtcaggt ctatgttcgc accgtagcgg cgaataacgc ccaaccgcta
    10201 acccaggatc aatgcttacg cgcgctgcat cacttacgtg ttcaatccac cagcaaaact
    10261 gcaccacagg cgaaataatc tcatctttgc taatgcgagg ctggagccat ccagcctgaa
    10321 atgactgata acgtgatcgt tggtcatgcc ggatgcggcg taaacgcctt atccggccta
    10381 caaattctca agcattcaat cagttgcaat gccttatgta ggcctgatag cgaagcgcat
    10441 ctggcaatta tacgtttgtc accatcacat cagttcgggc acttatatac ctggccgata
    10501 atctggctat ccgtcgggac aaaactggac aacattccct gcgacgggct actgatgcca
    10561 taaatcacgt taccgcccat tgcagccgcc tggttgcgca gatcgtttgc tgcgccgcgc
    10621 atagaaccgc cctcttctcc gtgttgcccg gaaagccagt tgctttgctt acctgtcgca
    10681 gtaccaatca gctggcactc tgcgcctggc tgctcgtcca caatgcgtac actctgaccg
    10741 gcagcactca attcgttgct ggagctacaa cccgccatca gtagcgcggc accgacaatc
    10801 cctgctaagt attttacgtg catgttattc cccataatca atgagctgga cgccactcgc
    10861 gtcgtccgtg tctgaatctt atactagaaa gatgaccaaa agaaaaaccc ccagacattt
    10921 ctgcccgggg gtttgtttat ttctgcgagg tgcagggcaa ttacatcatg ccgcccatgc
    10981 cacccatgcc acccatgccg ccagcagcgc ctaagtcagc tgcatcgttt ttcggcaggt
    11041 cggtaaccat gcattcggtg gtgatcatca ggccagccac agaagccgcg tactgcagag
    11101 cagaacgggt tactttggtt gggtccagga tacccatgtc gatcatgttg ccgtattctt
    11161 cggttgctgc gttgtaaccg tagttgccgt cgccgccttt aacggtgtta gcaacaacag
    11221 acggttcttc gccgcagttc aggacgatct gacgcagcgg agcttccatt gcacgcagtg
    11281 caactttgat acccacgttc tggtcttcgt tctgaccacg caggtcagcc agtttagacg
    11341 ctacgcggat cagcgcaaca ccaccaccag caaccacgcc ttcttctacc gcagcacggg
    11401 tcgcgtgcag ggcgtcttca acgcgtgctt ttttctcttt catttcaact tcggtagcag
    11461 cacctacttt gataactgca acgccgcctg ccagtttcgc tacgcgctcc tgcagttttt
    11521 cacggtcgta gtcagaagtt gcttcttcaa tctgctgacg gatctgagca acacggccct
    11581 ggattgcagc ttcttcgccc acgccatcga tgatggtggt ggtgtctttg ttgatcacaa
    11641 cgcgtttagc ctgacccagg tcttccaggg ttgctttttc cagctccata ccgatctctt
    11701 cagagattac ggtaccgcca gtcagggttg cgatatcctg cagcatagct ttacgacgat
    11761 cgccgaagcc cggagcttta actgcagcaa ctttcacgat gccacgcatg gtgttaacaa
    11821 ccagagttgc cagcgcttcg ccttctacat cttcagcgat gatcagcagc ggtttgcctg
    11881 ctttggcaac ggcttccaga accggcagca tttcgcggat gttggagatt ttcttgtcag
    11941 ccagcaggat gaacgggctt tccagttcta ctgcgccagt ttccggcttg ttgatgaagt
    12001 aaggagacag gtagccacgg tcgaactgca taccttcaac cacgtccagt tcgtcctgca
    12061 gaccggtacc gtcttcaacg gtgataacgc cttctttacc gactttgtcc atcgcttcag
    12121 cgatcagttt acctacggtt tcgtcggagt tagcggagat agtaccaacc tgagcaatcg
    12181 ctttagagtc agagcacggt acggacagcg ctttcagttc ttcaactgca gcggtaacag
    12241 ctttgtcgat accacgtttc aggtccatcg ggttcatgcc cgcagcaaca gctttcagac
    12301 cttcagtgat gatagcctga gccagtacgg ttgcagtggt ggtaccgtcg cctgcagcgt
    12361 cgttcgcttt agaggcaact tctttcacca tctgcgcacc catgttttcg aacttgtctt
    12421 ccagttcgat ttcacgagca acggaaacac catctttggt gatggtcggt gcaccgaaag
    12481 atttatccag aactacgtta cggcctttcg gaccgagggt aactttcact gcatctgcca
    12541 gtacgtttac gccgcgcagc attttcacac gagcgtcgtt accgaatttt acgtctttag
    12601 ctgccattat ctttattcct taaattcgta tgttcagtgt cgtgcgagga ttacgcttca
    12661 acaattgcca gaatgtcgct ttcggacatg atcaacactt cttcattgtc gatcttctcg
    12721 gatttcacac cgtagccatc gttgaaaata acgatgtcgc caactttcac atccagcggc
    12781 ttcacttcgc cattttcaag gatacggcca ttgccgacag ccagcacttc gccgcgggtg
    12841 gatttagccg ctgcagagcc ggtcagaacg atgccgccag cagatttagt ttcaacttct
    12901 ttacgcttga cgatcacgcg atcatgcaat ggacgaatat tcattgataa ctctcctttg
    12961 agaaagtccg tatctgttat gggtgacgcc ggagcttacg tggtttcccg gctggtgacc
    13021 agagaaatgg ggatgaggct tcgccccttc aagggggaaa aagaaaaaaa attctgatca
    13081 gcacaaaatc gggtgaaaac cctgattcac ctcacatttc atcgcaattt cttcatcgcc
    13141 gtcataagcg aatctgattg tgctaccatc gaaaatctac gcagttgccc aaaatttggg
    13201 cgcaatcgga catcaaccca tgagtggact caaacaagaa ctggggctgg cccagggcat
    13261 tggcctgcta tcgacgtcat tattaggcac tggcgtgttt gccgttcctg cgttagctgc
    13321 actggtagcg ggcaataaca gcctgtgggc gtggcccatt ttgattatct tagtgttccc
    13381 gattgcgatt gtgtttgcga ttctgggtcg ccactatccc agcgcaggcg gcgtcgcgca
    13441 cttcgtcggt atggcgtttg gttcgcggct tgagcgagtc accggctggc tgtttttatc
    13501 ggtcattccc gtgggtttgc ctgccgcgct acaaattgct gccggattcg gccaggcaat
    13561 gtttggctgg catagctggc aactgttgtt ggcagaactc ggtacgctgg cgttggtgtg
    13621 gtatatcggt actcgcggtg ccagttccag tgcaaatcta caaacagtta ttgccggact
    13681 tatcgtcgca ctgattgtcg ctatctggtg ggcgggcgat atcaaacctg cgagtatccc
    13741 cttccccgcg ccaggaaata tcgaacttac cgggttattt gctgcgttat cagtgatgtt
    13801 ctggtgtttt gtcggtctgg aagcatttgc ccatcttgcc tcggaattta aaaatccaga
    13861 acgtgatttt cctcgtgctt tgatgattgg tctgctgctg gcaggattag tctactgggg
    13921 ctgtacggta gtcgtcttac acttcgacgc ctatggtgaa caaatggccg cggcagcatc
    13981 gcttccaaaa attgtagtac aactgttcgg tgtaggagcg ttatggattg cctgcgtaat
    14041 tggctatctg gcctgctttg ccagtctcaa catttatata cagagcttcg cccgcctggt
    14101 ctggtcgcag gcgcaacata atcctgacca ctacctggca cgcctctctt ctcgccatat
    14161 cccgaataat gccctcaatg cggtgctcgg ctgctgcgtg gtgagcacgt tggtgattca
    14221 tgctttagag atcaatctgg acgctcttat tatttatgcc aatggcatct ttattatgat
    14281 ttatctgtta tgcatgctgg caggctgtaa attattgcaa ggtcgttatc gactactggc
    14341 ggtggttggc gggctgttat gcgttctgtt actggcaatg gtcggctgga aaagtctcta
    14401 tgcgctgatc atgctggcgg ggttatggct gtttctgcca aaacgaaaaa cgccggaaaa
    14461 tggcataacc acataatccg gcgtttcgac attaatcctg gcgatcgtct ttatgatcaa
    14521 ggcggtcgcg gtcatcatcc tttcgctggt actcaccatc aaaagtatta ccgccaccgg
    14581 tcccggcgct aaaaccgccg cctggcatgc gagaaaagcg caaatgcggc atcaacttca
    14641 ctgtcagatg cttttgcacc ggcggcaata aaagcagaag accgaggaag tcggtgaaaa
    14701 agcccggtaa taaaagcagc aaaccggcaa tgatcagcga aacactttta atcatctccg
    14761 ccgctgggtt ttcacccgcc gccatttttt gctgcatcag cacgaaattc ttaaagccct
    14821 ggttacgtac cagtgacata ccgataactg acgtaaatat aaccagcacg agagtcagca
    14881 atacccccaa tacatgggca acctgaataa agattgaaat ctcaatatag acataaagga
    14941 aaatggcaat aaaaggtaac cagcgcaaag gtttctcctg taatagcagc cggttaaacc
    15001 cggctacctg aatgggttgc gaatcgcgtt cagcttatat tgtggtcatt agcaaaattt
    15061 caagatattt gcgcaactat ttttggtagt aatcccaaag cggtgatcta tttcacaaat
    15121 taataattaa ggggtaaaaa ccgacactta aagtgatcca gattacggta gaaatcctca
    15181 agcagcatat gatctcggat attcggtcga tgcaggggat aatcgtcggt cgaaaaacat
    15241 tcgaaaccac atatattctg tgtgtttaaa gcaaatcatt ggcagcttga aaaagaaggt
    15301 tcacatgtca aacaacattc gtatcgaaga agatctgttg ggtaccaggg aagttccagc
    15361 tgatgcctac tatggtgttc acactctgag agcgattgaa aacttctata tcagcaacaa
    15421 caaaatcagt gatattcctg aatttgttcg cggtatggta atggttaaaa aagccgcagc
    15481 tatggcaaac aaagagctgc aaaccattcc taaaagtgta gcgaatgcca tcattgccgc
    15541 atgtgatgaa gtcctgaaca acggaaaatg catggatcag ttcccggtag acgtctacca
    15601 gggcggcgca ggtacttccg taaacatgaa caccaacgaa gtgctggcca atatcggtct
    15661 ggaactgatg ggtcaccaga aaggtgaata tcagtacctg aacccgaacg accatgttaa
    15721 caaatgtcag tccactaacg acgcctaccc gaccggtttc cgtatcgcag tttactcttc
    15781 cctgattaag ctggtagatg cgattaacca attgcgtgaa ggctttgaac gtaaagcggt
    15841 cgaattccag gacatcctga aaatgggtcg tacccagctg caggacgcag taccgatgac
    15901 cctcggtcag gaattccgcg ctttcagcat cctgctgaaa gaagaagtga aaaacatcca
    15961 acgtaccgct gaactgctgc tggaagttaa ccttggcgca acagcaatcg gtactggtct
    16021 gaacacgccg aaagagtact ctccgctggc agtgaaaaaa ctggctgaag ttactggctt
    16081 cccatgcgta ccggctgaag acctgatcga agcgacctct gactgcggcg cttatgttat
    16141 ggttcacgga gcgctgaaac gcctggctgt gaagatgtcc aaaatctgta acgacctgcg
    16201 cttgctctct tctggtccac gtgccggcct gaacgagatc aacctgccgg aactgcaggc
    16261 gggctcttcc atcatgccag ctaaagtaaa cccggttgtt ccggaagtgg ttaaccaggt
    16321 atgcttcaaa gtcatcggta acgacaccac tgttaccatg gcagcagaag caggtcagct
    16381 gcagttgaac gttatggagc cggtcattgg ccaggctatg ttcgaatccg ttcacattct
    16441 gaccaacgct tgctacaacc tgctggaaaa atgcattaac ggcatcactg ctaacaaaga
    16501 agtgtgcgaa ggttacgttt acaactctat cggtatcgtt acttacctga acccgttcat
    16561 cggtcaccac aacggtgaca tcgtgggtaa aatctgtgcc gaaaccggta agagtgtacg
    16621 tgaagtcgtt ctggaacgcg gtctgttgac tgaagcggaa cttgacgata ttttctccgt
    16681 acagaatctg atgcacccgg cttacaaagc aaaacgctat actgatgaaa gcgaacagta
    16741 atcgtacagg gtagtacaaa taaaaaaggc acgtcagatg acgtgccttt tttcttgtga
    16801 gcagtaactt aaaaataaca atctaatatc aacttgttaa aaaacaagga aggctaatat
    16861 gctagttgta gaactcatca tagttttgct ggcgatcttc ttgggcgcca gattggggg
//
LOCUS       DAZKIP010000457         8784 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.guided.160, whole genome shotgun sequence.
ACCESSION   DAZKIP010000457 DAZKIP010000000
VERSION     DAZKIP010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8784
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.guided.160"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            99..302
                     /locus_tag="ACHOUK_005244"
                     /pseudo
     CDS             99..302
                     /locus_tag="ACHOUK_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            301..822
                     /gene="prfH"
                     /locus_tag="ACHOUK_005245"
                     /pseudo
     CDS             301..822
                     /gene="prfH"
                     /locus_tag="ACHOUK_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(879..2336)
                     /gene="pepD"
                     /locus_tag="ACHOUK_005246"
     CDS             complement(879..2336)
                     /gene="pepD"
                     /locus_tag="ACHOUK_005246"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFP9900461.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2597..3055
                     /gene="gpt"
                     /locus_tag="ACHOUK_005247"
     CDS             2597..3055
                     /gene="gpt"
                     /locus_tag="ACHOUK_005247"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFP9900462.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3147..4391
                     /gene="frsA"
                     /locus_tag="ACHOUK_005248"
     CDS             3147..4391
                     /gene="frsA"
                     /locus_tag="ACHOUK_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFP9900463.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSCLLHQRVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            4449..4850
                     /gene="crl"
                     /locus_tag="ACHOUK_005249"
     CDS             4449..4850
                     /gene="crl"
                     /locus_tag="ACHOUK_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFP9900464.1"
                     /translation="MTLPSGHPKSRLIKKFTVLGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(4889..5944)
                     /gene="phoE"
                     /locus_tag="ACHOUK_005250"
     CDS             complement(4889..5944)
                     /gene="phoE"
                     /locus_tag="ACHOUK_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFP9900465.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            6232..7335
                     /gene="proB"
                     /locus_tag="ACHOUK_005251"
     CDS             6232..7335
                     /gene="proB"
                     /locus_tag="ACHOUK_005251"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFP9900466.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7347..8600
                     /gene="proA"
                     /locus_tag="ACHOUK_005252"
     CDS             7347..8600
                     /gene="proA"
                     /locus_tag="ACHOUK_005252"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFP9900467.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVVVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIAYSFLPALSKQ
                     MAESGVTLHADASALAQLQTGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
BASE COUNT         2125 a         2188 c         2314 g         2157 t
ORIGIN      
        1 tgaatcccca ggagcttaca taagtaagtg actggggtga acgaacgcag tcgcagtaca
       61 tgcaacttga agtatgacga gtataaatgg aatggtacat gggcaaatat attcgtccct
      121 tatccgatgc ggtatttacc atcgcatctg atgacctgtg gatcgagagt ttagcgatcc
      181 aacaattaca caccacggca aatttaccca acatgcagcg cgtagtgggg atgccagatt
      241 tacaccccgg acgcggctac ccgattggcg cagcgttctt ctctgttggt cgtttttacc
      301 cgacaagacg tcgcggtaac ggtgctggaa acagaaacgg gccgctactc tgacacgctg
      361 cgttcggcgc tgatttctct ggatggcgac aacgcatggg cgttaagcga aagttggtgc
      421 ggcactattc agtggatttg tctgagtccg tatcggcctc atcatgggcg caaaaactgg
      481 tttctgggca ttgggcgttt taccgctgat gagcaggaac aatcggatgc aatccgttat
      541 gagacgctgc gtccgtcggg gccgggcggt caacatgtca ataaaaccga ctcggcggta
      601 cgcgccacgc atctggcaac cgggattagc gtgaaggttc agtcagaacg cagccagcat
      661 gctaacaaac ggctggcgcg attgctgatt gcctggaagc tggagcagca gcaacaggaa
      721 aatagcgcag tgctgaaatc gcagcggcga atgttccatc accagattga acgtggcaac
      781 ccgcgacgaa cgttcacagg gatggctttt atcgaaggat aatgaaggat gaaactgccg
      841 gaaggcgatt aaacgccatc cggcagcaaa tcaaataatt acttcgccgg aatttctttc
      901 agcagttcag tcagcagtgt ccagtaatga cctacgcttt cgatgtgaac ttgctcatcc
      961 ggagagtgtg gaccggtgat tgttggcccg atagaaacca tgtccatttc cggatacggt
     1021 tttttgaaca gaccacattc caggcccgcg tggataatct ggatgttcgg cgtcttgttg
     1081 aacaggcgct gataggtttc acgtaccaga tgcatcaccg gagaattagc gtccggctgc
     1141 cagccaggat atgcgccttt cgcttcggtt ttcgcgccag ccagtttacc cagcgaatcc
     1201 agcatgctca ccacgtagtc tttaccgctg tcgatcagag aacggatcag acagtgaatt
     1261 tctacgttat tgtcagtcat ggtcaccaca ccgacgttca gggaggtttc gaccacacct
     1321 tttgccacgt cggagttgcg gatcacaccg ttcggggtgg cgttcagcag acgaataaag
     1381 gtatcgcgag atttcgcaat cagggcagct ttatcgttcg ctacagagtc cagcaacaag
     1441 gccagatttt tctctttctc tgccagctcg tttttcagga tctcctgata ggtattcacc
     1501 agagatttca gggcgtcgac tttatcagct gcgacagcaa tggtcgcaaa ggcttcacgc
     1561 gggatggcgt tacgcagtgt gccgccgttg aaatcaataa gacgcaggtc cagttcttct
     1621 gcatgacccg ccaggaagcg caccagcagt ttgttggcat tacccaggcc aacgtggatt
     1681 tccccgccgg agtgaccgcc tttcagacct tttaaggtta acttgaaggt ttcaaaacca
     1741 gctggaaccg cttcacgatc taaatgcagg ttggaggtga agtcgatacc ccccgcgcaa
     1801 cccatgtaga tttcaccttc ttcttcggag tcggtgttaa tcagaatatc agcctgcaac
     1861 cagttgctct gtaagccgaa cgcaccgtcc ataccggctt cttcggtcat ggtcagcagc
     1921 acttccagcg ggccgtgaac cacgttttcg tcagccagaa ccgccagtgc agaggccata
     1981 ccaatgccgt tatccgcacc cagcgtggtg ccgcgcgctt taacccattc gccatcaata
     2041 tatggctgga taggatcttt cgtgaagtca tgcacggtgt cgttattttt ctgcggcacc
     2101 atatcgaggt gggcctgcaa gacgaccggt ttacgatttt ccatacctgc ggtagcaggt
     2161 ttacgaatca ggatattacc tacctgatcg cgttcgacat ggaaaccttt ctcttttgcc
     2221 caaccaacaa tgtattcagc gagttgctct tcatgatagg acgggtgagg aatagaacag
     2281 attttggcaa aaatatccca cagcggctgt ggagataatt gagacagttc agacacgtta
     2341 agtctccttg tcgatcaccc gcaaaacagt attgcaggtc acagggttag cagaaaatgt
     2401 tgtcaacaca agacaggctt gcgagatatg tttgagaata ccactttatc ccgcgtcagg
     2461 gagaggcagt gcgtaaaaag acgcggactc atgtgaaata ctggttttta gtgcgccaga
     2521 tctctataat ctcgcgcaac ctattttccc ctcgaacact ttttaagccg tagataaaca
     2581 ggctgggaca cttcacatga gcgaaaaata catcgtcacc tgggacatgt tgcagatcca
     2641 tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca ttattgccgt
     2701 aagccgtggc ggtctggtac cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt
     2761 cgataccgtt tgtatttcca gctacgatca cgacaaccag cgcgagctta aagtgctgaa
     2821 acgcgcagaa ggcgatggcg aaggcttcat cgttattgat gacctggtgg ataccggtgg
     2881 tactgcggtt gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tctttgcaaa
     2941 accggctggt cgtccgctgg ttgatgatta tgttgttgat atcccgcaag atacctggat
     3001 cgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt
     3061 tcaacgcctg gcactgccgg gcgttgttct ttttaacttc aggcgagtta caatagtttc
     3121 cagtaagtat tctggaggct gcatccatga cacaggcaaa cctgagcgaa accctgttca
     3181 aaccccgctt taaacatcct gaaacctcga cgctagtccg ccgctttaat cacggcgcac
     3241 aaccgcctgt gcagtcggcc cttgatggta aaaccatccc tcactggtat cgcatgatta
     3301 accgtctgat gtggatctgg cgcggcattg acccacgcga aatcctcgac gtccaggcac
     3361 gtattgtgat gagcgatgcc gaacgtaccg acgatgattt atacgatacg gtgattggct
     3421 accgtggcgg caactggatt tatgagtggg ccacccaggc gatggtgtgg caacaaaaag
     3481 cctgtgcgga agaagatccg caactcagtg gtcgtcactg gctgcatgcg gctacgttgt
     3541 acaacattgc cgcctatcct catctgaaag gagatgacct ggccgagcaa gcgcaggctt
     3601 tgtcaaaccg cgcctatgaa gaggccgctc agcgtctacc gggcacgatg cgacagatgg
     3661 agtttaccgt acccggcggt gcgcccatca ccggcttttt gcatatgccg aaaggcgatg
     3721 gcccgttccc gacggtatta atgtgtggtg gtctggatgc gatgcagacg gactattaca
     3781 gcctgtatga acgttatttt gcgccgcgcg gcattgcgat gctgactatt gatatgccgt
     3841 cggtgggctt ttcttcaaaa tggaagctca cccaggactc ctgcctgttg catcagcgcg
     3901 tcttaaaggc tctgcctaac gtaccgtggg tggatcacac tcgcgtcgcg gcctttggtt
     3961 tccgtttcgg cgctaacgtt gccgtgcgtc tggcatatct tgaatcgccg cgtctgaaag
     4021 cggttgcctg tcttggtccg gtagttcata ccctgttgag tgattttaag tgccagcaac
     4081 aggtgccgga aatgtatctt gacgttctgg cgagtcgttt ggggatgcat gatgcttccg
     4141 acgaagcgtt gcgcgtggag ctgaatcgct attcattaaa agtgcaagga ttgctgggac
     4201 gtcgatgccc aacgccaatg ttatcaggct actggaagaa cgatccgttc agcccggaag
     4261 aggactcacg cttaatcacc tcatcatctg ctgacggtaa attattagag atcccattta
     4321 acccggtgta tcggaatttt gacaaaggtc ttcaggaaat caccgactgg atcgaaaaac
     4381 gcttgtgtta aaaatttgct aaattttgcc aatttggtaa aacagttgca tcacaacagg
     4441 agatagcaat gacgttaccg agtggacacc cgaagagcag attgatcaaa aaatttaccg
     4501 tactaggccc gtatattcgt gaaggtaagt gcgaagataa tcgattcttt ttcgattgtc
     4561 tggctgtatg cgtcaacgtg aaaccggcac cggaagtgcg tgagttctgg ggctggtgga
     4621 tggagcttga agcgcaggaa tcccgtttta cctacagtta ccagtttggt ctgttcgata
     4681 aagcaggcga ctggaagagt gttccggtaa aagatactga agtggttgaa cgactggagc
     4741 acaccctgcg tgagtttcac gagaagctgc gtgaactgct gacgacgctg aatctgaagt
     4801 tggaaccggc ggatgatttt cgtgacgagc cggtgaagtt aacggcgtga gtgaaatgtg
     4861 ccggatgcat cacatccggc aataatcatt aaaactgata ggtcatgcca accgcgacaa
     4921 tatcatcatt attaatattc aatttgttat cgctatccag ttggttgatt ttataatcaa
     4981 caaacgctga catatttttg ttgaaataat atgtagcccc gacatcgata taattgacca
     5041 gatcttcatc accgatacct tcaatatctt tccctttcga taagacataa cccagcgatg
     5101 gacgcagacc aaagtcaaac tggtattgag cgaccgcttc aaagttctgt gtcttattgg
     5161 caaagccgcc agttattggc gtcattttgc gtgtttcaga ataaaaagtt gccagataaa
     5221 tattattggc atcgtatttc agacctgtag cccaagcttc tgcacgcttg cctgtgccac
     5281 ggctttgcag gttctgctcg ttggtgcgat ctgagttggt ataggcccca ctaatggcga
     5341 aatcgctgcc gccaaagtca tatgtcaatg acgtgccgaa gccatcgccg ttttgctttt
     5401 taacgtcgcg gttttcgttt ttcccttgat attgcagggt taagttcagg ccatcgataa
     5461 cgccgaagaa gtcggtgttc cgatacgtcg ccagaccgct ggcgcgtttg gtcataaagt
     5521 tgtcggtctg cgccgaggag tcgccaccaa attccgggaa catatcggtc caggcttcca
     5581 cgtcatacaa cgcgcccagg ttacggccat agtcgaaaga acccaaatct ttatacttca
     5641 atccggcaaa agcgagacgc gttttttgct gtgcagtatc actctccgct ttatttccgg
     5701 caaactccgc ttcccaacga ccataaccag tcagttgatc gttaatttgt gtttcgcctt
     5761 taaaaccaaa acggatataa ctctggtcgc catctttact gtcgttatca ctcatataat
     5821 gcatggcttt aactttgcca tagacatcca gtttattacc gtctttgtta tatatttctg
     5881 cggcctgtac ggatgcagat gccacaatgc ccatcaccac taatgccaga gtgctctttt
     5941 tcattttcat tcctgatttt aattaacgcg cgaatattct gcgggagagc cccgttgaaa
     6001 acaggaaagt ttttaacctg agattgttaa agatatatta cagattaatg atattcttaa
     6061 aatgtggtaa tttattaaat ctgtaataaa agcgtaaaca actgccgcta ggcttgctga
     6121 tcccgcgcaa caaaacgcca tgctttgctc gcagatggtt ggcaaccgac gacagtcctg
     6181 ctaaaacgtt cgtttgatat catttttcct aaaattgaat ggcagagaat catgagtgac
     6241 agccagacgc tggtggtaaa actcggcacc agtgtgctaa caggcggatc gcgccgcctg
     6301 aaccgtgccc atatcgttga acttgttcgc cagtgcgcgc agttacatgc cgccgggcat
     6361 cggattgtta ttgtgacgtc gggcgcgatc gccgccggac gtgagcacct gggttacccg
     6421 gaactgccag cgactatcgc ctcgaaacaa ctgctggcgg cggtagggca gagtcgactg
     6481 attcaactgt gggaacagct gttttcgatt tatggcattc acgtcgggca aatgctgctg
     6541 actcgtgctg atatggaaga ccgtgaacgc ttcctgaacg cccgcgacac cctgcgtgcg
     6601 ttgctcgata acaatatcgt tccggtaatc aatgagaacg atgctgtcgc tacggcagag
     6661 attaaagtcg gcgataacga caacctttct gcactggcgg cgattctggc gggtgccgat
     6721 aaactgttgt tactgaccga tcaaaaaggt ttgtataccg ctgacccgcg cagcaatccg
     6781 caggcagaac tgattaaaga tgtttacggc attgatgacg cactgcgcgc gattgctggt
     6841 gacagcgttt caggcctcgg aactggcggc atgagtacca aattgcaggc cgctgacgtg
     6901 gcttgccgtg cgggtatcga caccattatt gccgcgggca gcaagccggg cgttattggt
     6961 gatgtgatgg aaggcatttc cgtcggtacg ctgttccatg cccaggcgac tccgcttgaa
     7021 aaccgtaaac gctggatttt cggtgcgccg cctgcgggtg aaatcacggt agatgaaggg
     7081 gcaaccgccg ccattcttga acgcggcagc tccctgttgc cgaaaggcat taaaagcgtg
     7141 actggcaact tctcgcgtgg tgaagtcatc cgcatttgta acctcgaagg tcgcgatatc
     7201 gcccacggcg tcagtcgtta caacagcgat gcattacgcc gtattgccgg acaccactcg
     7261 caagaaattg atgcaatact gggatatgaa tacggcccgg ttgccgttca ccgtgatgac
     7321 atgatcaccc gttaaggagc aggctgatgc tggaacaaat gggcattgcc gcgaagcaag
     7381 cctcgtataa attagcgcaa ctctccagcc gcgaaaaaaa tcgcgtgctg gaaaaaatcg
     7441 ccgatgaact ggaagcacaa agcgaaatca tcctcaacgc taacgcccag gatgttgctg
     7501 acgcgcgagc caatggcctt agcgaagcga tgcttgaccg tctggcactg acgcccgcac
     7561 ggctgaaagg cattgccgac gatgtacgtc aggtgtgcaa cctcgccgat ccagtggggc
     7621 aggtaatcga tggcggcgta ctggacagcg gcctgcgtct tgagcgtcgt cgcgtaccgc
     7681 tgggggttat tggcgtgatt tatgaagcgc gcccgaacgt gacggttgat gtcgcttcgc
     7741 tgtgcctgaa aaccggtaac gcggtgatcc tgcgtggtgg caaagaaacc tgtcgcacta
     7801 acgcggcaac ggtggtggtg attcaggacg ccctgaaatc ctgtggctta ccggcgggtg
     7861 ccgtgcaggc gattgataat cctgaccgtg cgctggtcag tgaaatgctg cgtatggata
     7921 aatacatcga catgctgatc ccgcgcggcg gggctggttt gcataaactg tgccgcgagc
     7981 agtcgacgat cccggtgatc acaggtggta taggcgtatg ccatatttat gttgatgaaa
     8041 gtgcagagat tgctgaagcc ctgaaagtaa tcgtcaatgc gaaaactcag cgtccgagca
     8101 catgtaatac ggtagaaacg ttgctggtga ataaaaacat cgcctatagc ttcctgcccg
     8161 cattaagcaa acaaatggcg gaaagcggcg tgacgttaca cgcagatgca tctgcgctgg
     8221 cgcagttgca gacaggccct gcgaaggtgg tggcggttaa agccgaagag tatgacgatg
     8281 agtttctgtc attagatttg aacgtcaaaa tcgtcagcga tcttgacgat gccatcgccc
     8341 atattcgtga acacggcacg caacactccg atgcgatcct gacccgcgat atgcgcaacg
     8401 cccagcgttt tgttaacgaa gtggattcgt ccgctgttta cgttaacgcc tctacgcgtt
     8461 ttaccgacgg cggccagttt ggactgggtg cggaagtggc ggtaagcaca caaaaactcc
     8521 acgcgcgtgg cccaatgggg ctggaagcac tgaccactta caagtggatc ggcattggtg
     8581 attacaccat tcgtgcgtaa ataaaaccgg gtgatgcaaa agtagccgtt tgattcacaa
     8641 ggccattgac gcatcgcccg gttagtttta accttgtcca ccgtgattca cgttcgtgaa
     8701 catgtccttt cagggccgat atagctcagt tggtagagca gcgcattcgt aatgcgaagg
     8761 tcgtaggttc gactcctatt atcg
//
LOCUS       DAZKIP010000458         2748 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.guided.265, whole genome shotgun sequence.
ACCESSION   DAZKIP010000458 DAZKIP010000000
VERSION     DAZKIP010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.guided.265"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..110
                     /locus_tag="ACHOUK_005253"
     CDS             <1..110
                     /locus_tag="ACHOUK_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFP9900468.1"
                     /translation="GFWLDDAQVVDYRVVKMPVTKGGRLELTITEMGNE"
     gene            107..247
                     /locus_tag="ACHOUK_005254"
     CDS             107..247
                     /locus_tag="ACHOUK_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFP9900469.1"
                     /translation="MMFEFNMAELLRHRWGRLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            333..767
                     /locus_tag="ACHOUK_005255"
     CDS             333..767
                     /locus_tag="ACHOUK_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFP9900470.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLIPEKVKSRPQ
                     CCDDDAMVICGCIARLYRNNRDLHDLLVDYYVLGETFMALARKHGCSDTCIGKRLHKA
                     EGIVEGMLMMLGVRLEMDRYVERELPGGRTSVFYQRKNSLRS"
     gene            1274..2221
                     /gene="stxA1a"
                     /locus_tag="ACHOUK_005256"
     CDS             1274..2221
                     /gene="stxA1a"
                     /locus_tag="ACHOUK_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
                     /protein_id="HFP9900471.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGTGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMAS
                     DEFPSMCPADGRVRGITHNKILWDSSTLGAILMRRTISS"
     gene            2231..2500
                     /gene="stxB1a"
                     /locus_tag="ACHOUK_005257"
     CDS             2231..2500
                     /gene="stxB1a"
                     /locus_tag="ACHOUK_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HFP9900472.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
BASE COUNT          720 a          492 c          706 g          830 t
ORIGIN      
        1 caggtttctg gctggatgat gctcaggtcg ttgattaccg cgttgtgaag atgcctgtta
       61 ccaaaggtgg gaggctggaa ctgaccatca ccgaaatggg gaatgaatga tgtttgagtt
      121 taatatggca gaacttcttc gccaccgctg ggggcgtctg cgcttatatc gtttccccgg
      181 ttctgttttg accgattacc gaatactgaa gaattacgcc aaaaccctga caggagcagg
      241 agtatgaagt cagagataac aatcaactaa tactgttttg ttgatttttg cttgtaattg
      301 gcgttctggt ctgatttttg tggagtaagt tgatgcgtga tattcagatg gttcttgagc
      361 gttggggagc gtgggcggct aataatcatg aagatgtgac ctggtcgtcc attgccgccg
      421 gttttaaggg actgatcccc gaaaaagtaa aatcacgccc gcagtgctgt gacgatgatg
      481 cgatggtgat atgcgggtgt atagcccgcc tttaccggaa caatcgcgat ctgcatgact
      541 tgctggttga ttattacgtg ttgggggaga cgttcatggc gctggcacgg aaacatgggt
      601 gctctgacac ctgtataggt aaacgccttc acaaagcgga ggggattgtt gaaggcatgc
      661 tgatgatgct gggagtgagg cttgagatgg atcggtatgt tgagcgtgaa ttgccgggag
      721 ggagaacctc tgtattttat cagcgaaaaa atagtttacg atcgtaaaaa tctgcatatc
      781 atgataagag tggttacatt gccacgctgc ttaacccgcc gatgcgcggg tttttttgta
      841 cccagaatcc tgtgagctat acggaaagta cacagaaagg aaggtgcgac cacaattaat
      901 aacaaaatct taaaaattgc acatggcact attagttttc taaatattgt gtattttttg
      961 tattgcagga tgaccctgta acgaagtttg cgtaacagca ttttgctcta cgagtttgcc
     1021 agcctccccc agtggctggc ttttttatgt ccgtaacatc ctgtgtatca ataaatgttg
     1081 ttatctacgt acgtcaagta gtcgcatgag atctgaccag atatgttaag gttgcagctc
     1141 tctttgaata tgattatcat tttcattacg ttattgttac gtttatccgg tgcgccgtaa
     1201 aacgccgtcc ttcagggcgt ggaggatgtc aagaatatag ttatcgtatg gtgctcaagg
     1261 agtattgtgt aatatgaaaa taattatttt tagagtgcta acttttttct ttgttatctt
     1321 ttcagttaat gtggtggcga aggaatttac cttagacttc tcgactgcaa agacgtatgt
     1381 agattcgctg aatgtcattc gctctgcaat aggtactcca ttacagacta tttcatcagg
     1441 aggtacgtct ttactgatga ttgatagtgg cacaggggat aatttgtttg cagttgatgt
     1501 cagagggata gatccagagg aagggcggtt taataatcta cggcttattg ttgaacgaaa
     1561 taatttatat gtgacaggat ttgttaacag gacaaataat gttttttatc gctttgctga
     1621 tttttcacat gttacctttc caggtacaac agcggttaca ttgtctggtg acagtagcta
     1681 taccacgtta cagcgtgttg cagggatcag tcgtacgggg atgcagataa atcgccattc
     1741 gttgactact tcttatctgg atttaatgtc gcatagtgga acctcactga cgcagtctgt
     1801 ggcaagagcg atgttacggt ttgttactgt gacagctgaa gctttacgtt ttcggcaaat
     1861 acagagggga tttcgtacaa cactggatga tctcagtggg cgttcttatg taatgactgc
     1921 tgaagatgtt gatcttacat tgaactgggg aaggttgagt agcgtcctgc ctgactatca
     1981 tggacaagac tctgttcgtg taggaagaat ttcttttgga agcattaatg caattctggg
     2041 aagcgtggca ttaatactga attgtcatca tcatgcatcg cgagttgcca gaatggcatc
     2101 tgatgagttt ccttctatgt gtccggcaga tggaagagtc cgtgggatta cgcacaataa
     2161 aatattgtgg gattcatcca ctctgggggc aattctgatg cgcagaacta ttagcagttg
     2221 agggggtaaa atgaaaaaaa cattattaat agctgcatcg ctttcatttt tttcagcaag
     2281 tgcgctggcg acgcctgatt gtgtaactgg aaaggtggag tatacaaaat ataatgatga
     2341 cgataccttt acagttaaag tgggtgataa agaattattt accaacagat ggaatcttca
     2401 gtctcttctt ctcagtgcgc aaattacggg gatgactgta accattaaaa ctaatgcctg
     2461 tcataatgga gggggattca gcgaagttat ttttcgttga ctcagaatag ctcagtgaaa
     2521 atagcaggcg gagattcata aatgttaaat acatctcaat tcagtcagtt gttgccggtc
     2581 tgataataga tgtgttagaa aatttctgca tggtgaatcc ccctgtgcgg aggggcgact
     2641 ggtgaacggt atgatctctt tgatgatcgt aagcgagaat acgcgggttt ggtggcacca
     2701 ggccgaactc accgggaggc acccggcatc atgctgtata cagagatt
//
LOCUS       DAZKIP010000459         2364 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H71338
            SAMN43267428-rid23075163.guided.277, whole genome shotgun sequence.
ACCESSION   DAZKIP010000459 DAZKIP010000000
VERSION     DAZKIP010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267428
            Sequence Read Archive: SRR30307196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350514.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:42:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 94
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 100 of 229
            Pseudo Genes (incomplete)         :: 124 of 229
            Pseudo Genes (internal stop)      :: 56 of 229
            Pseudo Genes (multiple problems)  :: 42 of 229
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267428-rid23075163.guided.277"
                     /serotype="O157:H 7"
                     /isolate="2103H71338"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            95..1054
                     /locus_tag="ACHOUK_005258"
     CDS             95..1054
                     /locus_tag="ACHOUK_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HFP9900473.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     DSQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            1066..1335
                     /locus_tag="ACHOUK_005259"
     CDS             1066..1335
                     /locus_tag="ACHOUK_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2c subunit B"
                     /protein_id="HFP9900474.1"
                     /translation="MKKMFMAVLFALVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
     gene            complement(1632..1955)
                     /locus_tag="ACHOUK_005260"
     CDS             complement(1632..1955)
                     /locus_tag="ACHOUK_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFP9900475.1"
                     /translation="MNWIVIDTVIQPACGISFSAIWCNIKLILWYQSDTFLPPGCIFT
                     PTRTGVILNNKELPVTIYNVAPFNKNVWNLIKNSQECPANTGNITDKCFNHRCILEIC
                     PYGRK"
     gene            2199..>2364
                     /locus_tag="ACHOUK_005261"
     CDS             2199..>2364
                     /locus_tag="ACHOUK_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFP9900476.1"
                     /translation="MTFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTT"
BASE COUNT          614 a          467 c          581 g          702 t
ORIGIN      
        1 cacacttatt ttccctggct cgcttttgcg ggcctttttt atatctgcgc cgggtctggt
       61 gctgattact tcagccaaaa ggaacacctt gtatatgaag tgtatattat ttaaatgggt
      121 actgtgcctg ttactgggct tttcttcggt atcctattcc cgggaattta cgatagactt
      181 ttcgactcaa caaagttatg tatcttcgtt aaatagtata cggacagaga tatcgacccc
      241 tcttgaacat atatctcagg ggaccacatc ggtgtctgtt attaaccaca ccccaccggg
      301 cagttatttt gctgtggata tacgagggct tgatgtctat caggcgcgtt ttgaccatct
      361 tcgtctgatt attgagcaaa ataatttata tgtggccggg ttcgttaata cggcaacaaa
      421 tactttctac cgtttttcag attttacaca tatatcagtg cccggtgtga caacggtttc
      481 catgacaacg gacagcagtt ataccactct gcaacgtgtc gcagcgctgg aacgttccgg
      541 aatgcaaatc agtcgtcact cactggtttc atcatatctg gcgttaatgg agttcagtgg
      601 taatacaatg accagagatg catccagagc agttctgcgt tttgtcactg tcacagcaga
      661 agccttacgc ttcaggcaga tacagagaga atttcgtcag gcactgtctg aaactgctcc
      721 tgtgtatacg atgacgccgg gagacgtgga cctcactctg aactgggggc gaatcagcaa
      781 tgtgcttccg gagtatcggg gagaggatgg tgtcagagtg gggagaatat cctttaataa
      841 tatatcggcg atactgggca ctgtggccgt tatactgaat tgtcatcatc agggggcgcg
      901 ttctgttcgc gccgtgaatg aagatagtca accagaatgt cagataactg gcgacaggcc
      961 cgttataaaa ataaacaata cattatggga aagtaataca gctgcagcgt ttctgaacag
     1021 aaagtcacag tttttatata caacgggtaa ataaaggagt taagtatgaa gaagatgttt
     1081 atggcggttt tatttgcatt agtttctgtt aatgcaatgg cggcggattg cgctaaaggt
     1141 aaaattgagt tttccaagta taatgagaat gatacattca cagtaaaagt ggccggaaaa
     1201 gagtactgga ccagtcgctg gaatctgcaa ccgttactgc aaagtgctca gttgacagga
     1261 atgactgtca caatcaaatc cagtacctgt gaatcaggct ccggatttgc tgaagtgcag
     1321 tttaataatg actgaggcat aacctgattc gtggtatgtg ggtaacaagt gtaatctgtg
     1381 tcacaattca gtcagttgac agttgcctgt cagactgagc attcgttaaa aaaattcgca
     1441 tggtgaatcc ccctgtgcgg aggggcgact ggtgtataag gtgtcatctc tgacgacaac
     1501 ctggagcgag atacgcgggt tcagggacac cggactgaac tcaccgggag gcacccggca
     1561 ccatgtacat gatgatacag atacgcagtg tcagcccctc tccggagggg ctttttatct
     1621 gaatgattct gttattttcg cccgtatggg catatttcca gaatgcagcg atgattaaaa
     1681 cacttatctg tgatatttcc tgtgtttgca gggcactcct ggctgttttt aattaaattc
     1741 cagacgtttt tattaaatgg ggccacgttg taaatggtga cggggagctc tttattattg
     1801 agtattacgc ctgtccgggt tggtgtaaaa atacagcccg gagggaggaa ggtatctgac
     1861 tgataccata atattaattt tatgttacac catatggctg aaaatgatat gccacatgct
     1921 ggctggatta ccgtgtcaat cactatccag ttcatttgct ttccttattt tcttaaggtt
     1981 ttaaatgatg ccataaatta taggtgatgt tttgaaagca atctgtcagg ctgagttgtt
     2041 tttctggtgt cgtttattct ttgtttaaaa taagagggca ttttaaattg tcggcgtttt
     2101 tttgttatat aagagacatg gtttttttat agcgtgtttt ttatgtgtta catgattgtg
     2161 tgttccggat aattactttg ctggcggcag gagaggttat gacatttaaa cactacgatg
     2221 tggtcagggc ggcatcgccg tcagaccttg ctgaacgact gacacaaaaa ctgaaggagg
     2281 gctggcagcc atttggcagt ccggtggcca tcacgcctta caccctgatg caggccattg
     2341 cggcggaagg tgatgtcacc acac
//
